LOCUS       DAOSCV010000001        17359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_2265_length_17359_cov_3.632975, whole genome shotgun sequence.
ACCESSION   DAOSCV010000001 DAOSCV010000000
VERSION     DAOSCV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 17359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17359
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_17359_cov_3.632975"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLY56_00005"
     CDS             complement(<1..705)
                     /locus_tag="PLY56_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ19896.1"
                     /translation="MGSPRFRVLVADDEEMVRESVCDVLRANGYEVCASVASGEAAVE
                     CASRLHPDVVLMDIRMPGMGGLDAARLIRGLPQPVPVVFLTALGDREMIERAADSGGF
                     GYLVKPIHGHQIGPMLHTAVHRFQELIQACGTKPSEAAVQPTLDRLAERARSAGLEGA
                     VEEFLASVCSQLEGVGTAIAITEPGALVPVVPACWGNVPPADAPAWTAESDEMPDITR
                     APILDADAHPMGTLFLF"
     gene            1555..2268
                     /locus_tag="PLY56_00010"
     CDS             1555..2268
                     /locus_tag="PLY56_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ19897.1"
                     /translation="MITIVLADDHAIVRDGLVPLLEAQPEFSVVAAVENGREALDAIG
                     RFQPDIAIIDILMPEVGGIDVVRQACEISPNTRAVILSAYSEERYLLAAFRAGARAFV
                     LKDARPRDLIAAITSVVAGQRYLSRPLAEAVLDAYLDQVEQSGACDPYEQLTTREREV
                     LHWTVEGKTAPQIASLLEISVRTVETHRANVMRKLGTRSKSDLVRYVVERAERQADPR
                     IRGGARRDPARHTTAANRR"
     gene            2393..4828
                     /locus_tag="PLY56_00015"
     CDS             2393..4828
                     /locus_tag="PLY56_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ19898.1"
                     /translation="MRVLLVENCDDDAILIHELLTKAPGGPYDIDWVERLSAAQDHFR
                     AAACDVVLLSLCLPDSQGLATVERMLQAAPEAPIVVITTLEDETVALSAVQRGAQDYL
                     VKDQLTPSFLGRVLRYAIERKEAERALRESEERFRAITENASDIVAVLSPEGNCLFIS
                     ASVENLLGCSAHAVQGTNVLQWVHPHDQDRTRSALQAVANLSRSAQPVTLRVRGASGS
                     WHWIEAKARNLLQHPAVRGIVANARDITEHHLAQEAINAQLSRLDLLNELTRAIADRQ
                     DLARMLPILLAHLEDSMALDGSAIYIYDGQADSLSLAASSGLLEGDPHQGDHGPPSRL
                     STEGLRSCLTRQSTYVRDYAESPETIPLLANAGLRSGVALPLHAGGMLYGLLVTARCQ
                     AHGFTSDEIEFLRRLGEHIALAIRQDTLLWDLHSAYKELQRTQQSALEQERLRILGQM
                     ASGIAHDINNALMPITTYAELLAMRVPDLDEHAQRYLKSILMAADDIKKTVGRMRELY
                     RKRDGRDQVRSIHIARLMRDVVDLTRPRWHDLPQQQGVTIAVKIETDGAPTYVTAVES
                     ELREALANLIGNAVDAMPIGGSITLRARPWSRSLLAIEVCDTGYGMDAETLRQCLEPF
                     FTAKGQSGTGLGLSMVQGIVQGHGGTIEIESQPGKGTTVRLLLPMDGPAAARRELPSS
                     GCCQSRPLRVLCVDDDDRVRAALMDMLEADGHIVVGAPGGEAGIQQFRHALLHGMPFD
                     IVLTDLGMPHIGGRMVALAIKAESPETPVVLLTGWGTFMEPGVFPEGIDAILGKPPEL
                     ASIRKVFAKLTQR"
     gene            complement(4922..5593)
                     /locus_tag="PLY56_00020"
     CDS             complement(4922..5593)
                     /locus_tag="PLY56_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ19899.1"
                     /translation="MKWRRDRQSGFTLIELLVVIAIIAILAAILFPVFAQARENARKS
                     NCQSNLKQIGTGILMYAQDYDEVLPPSYLYLDSAKTQLAWWEDLLQPYVKNYNVMACP
                     SDSDGVYANLRPAGTANPLKFSYAANGHGGGSGIRPMGGAGTSCSLSQIQTVSNLILI
                     GETRSTSTGGGKEWWSVDHADAYAADGVGLIDRRHSSGSNWLFADGHVKFLKKTEREM
                     WNPTK"
     gene            complement(5885..9184)
                     /locus_tag="PLY56_00025"
     CDS             complement(5885..9184)
                     /locus_tag="PLY56_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ19900.1"
                     /translation="MGAEAKPPTDWSDWERYRAGVQHPASSIKQADLERAQENVKRYP
                     WAQAYVDRLRASADTLVPRITPEYLVRMLERTTPGCVGPCPACRAKGLPWHPNGQWSW
                     NEQTPDQLTCNICKTVFPNEEFPEDIVLQSKWDPEQKFTFVGQGPPFRCFGYNARPSL
                     TGIIRARKVSQITSHLRTLAHAYALTQDARYARAARAILLRMAGVLPRYLVRAGYAYG
                     EYADCDPHVAARQISNLPTDELVVPPNKPDRKLHTGYWSASRLGTSGMDGGLICTITE
                     AYDLTCMAKEGDTPIFSDEDRVRIERDVLLEGAYLAACDPAINNKSVGNRAGAAMVGM
                     CVGHPGLVHFGLDGFRRTVEEWFLPDGGTSESAAYAMMTMGGVRNFALMFRGYSDPPG
                     YRGPDGTRLDNFDACRDTRYGDCWQGLVWTLQGDLFHPPSADSYRTTRINASFAELLA
                     LGYPTPEHIALLKELSGDEAPGSPQAAIFYREPGLEARSVPPLSLPDIVFPFLSQGNL
                     RTGENGRKSLALLNASDWGGHHHMDSLDLYYWKDGHELLSDLGYLWDHPDSYQTRRTF
                     AHNLVVVDRKDQKTRDRGGSFHLFGCTPRVKVMEASSRAYDAASTYRRTCIQVDHGDA
                     GSYLVDLFRVEGGTERDYVFHGPGGDYQVTGLALAPDGQVEREVRFALRFPLARVGEL
                     YVADVEIRRVMPGEQEGPNLIAPILGEVGSQPAGWGYYSGDGTGDWGIAPSDEAGGRC
                     MRVRALKPHENGRMNVALLAGKSDGYRGEGALSGSMGATYKARFRLRGNVPQVRVQAV
                     YWPNDPKSPDDRSYVNIINAPVDEGWQHYEVTFTLPTSVLPLTDVQRAEGGNPWQITW
                     SLEGDYTFAALAPGAPGETVRLGNGWGQRDHRNRDRGAVLPYVVRSSRGPKPVTFVTL
                     FAGAPKGKLPVRGIRVLPLPADTPSDAIALAVETALGTDIVLSQLQPRVLRVTTPLGE
                     LVTDGLAAAVLADGQAPVTTALFGGTRLSLGAASVTIARGRYAGRVAGTGSARGEAWF
                     VLDGELPKEGVRPGETLFIEADGMRRAYPLRRVAVVDGETRVYTKTEGTGFEARPGES
                     WEILPSASWGR"
     gene            9630..10097
                     /locus_tag="PLY56_00030"
     CDS             9630..10097
                     /locus_tag="PLY56_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOJ19901.1"
                     /translation="MQYTEGQIGRVFAIRLEHGDPMPQALEDFAAEKGVRSGLVILVG
                     GVEDGSRLVVGPEDGESLPPVPQVGTLKGVHEAAGVGTLFPDESGKPVLHLHAACGRG
                     EKTVTGCIRAGIVTWHVLEVLLVELTGLDAVRVADPVTKFQLLRCGEGSREQA"
     gene            10150..11760
                     /locus_tag="PLY56_00035"
     CDS             10150..11760
                     /locus_tag="PLY56_00035"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOJ19902.1"
                     /translation="MLDLVFVGGEVIDGTGRPGIRADLGVRDGRIAAIGDLSGVECRE
                     RVDARGKVVCPGFIDMHTHSDLTLLVNPRAESMVHQGVTTLVVGNCGMGVAPVRPELR
                     GPLRQAFAFLTGDVEWTWESFASYLDCLAAARPSVNVGALVSHGAVRAHVMSFDEREA
                     LPEERSAIAAEIRQAMAEGALGVSTGLIYNPSMFADTEELVAVSRAAAEGNGVYTTHM
                     RHEGVQLMEAVEEALEVARRSGARTEICHLKASGKAAWGRAAEAAERIRQAREEGNDI
                     AFDLYPYTAGSTMLSTLLPQWALRGTTDEIVARLRDPAAIARLLHELRHGGSGYHSGL
                     GAPPYEWERVLITSVGSEMNRGFEGKEMPEVARMMGATPEEAFLRLLVDDHLQVNMVI
                     HSQCEEDMRGLLKHPLALVITDGLAFAPYGPLGRGRPHPRSYGTFPRFLGRYVRDEGL
                     HSLEEGIRRVTSGPAARMGITDRGILREGLAADIVVFDPAAIRDTATYTEPHQYPEGI
                     DLVVVNGEIVVRDGEHTGAGPGQVLRKK"
     gene            complement(11826..12461)
                     /locus_tag="PLY56_00040"
     CDS             complement(11826..12461)
                     /locus_tag="PLY56_00040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ19903.1"
                     /translation="MGHLLLQPGARILFQGDSITDAGRSRENDALLGSGYANLIAAWL
                     SARYPGRELTFLNRGVSGNRVYDLEERWTRDCIDLHPDWVSILIGINDTWRRYDSGKL
                     SPIEEFEACYRRLLDRVKAETSARVILLEPFVLPTPPDRVAWREDLDPRIAAVRRVAA
                     DYGTLYVPLDGLFAAAAAQRPAAFWAADGVHPTQAGHALIAQAWIRAVDEG"
     gene            complement(12585..14240)
                     /locus_tag="PLY56_00045"
     CDS             complement(12585..14240)
                     /locus_tag="PLY56_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ19904.1"
                     /translation="MAKIIIDYVIPDRIVREEMGNRKMPLPPMSISLLRAMTPPSLRG
                     YDLIARCHDEHTMGPYDVRAQQPDIVAVSALTTAARRAYAILTEARETLSWQSRRVRS
                     LIGGIHATALPLEAIHYADGVVRGETPPVLLEAALEWCIEQIEEEGSERRVFEHAPNA
                     RMETLERRPIPDRSWYDPKRYLLPNTLQTSVGCPFDCSFCSVTYEYGMKQRHTDYEDL
                     EKEIAQLPRRLTAIIDDNFLPNPRGEHAKRVCQMLRHHRIPWVTELTALTLFRNWREL
                     VPLFSRSCCVGIYMGIESITAKLAKSTSLQNYIDLIRCIHDHGMGVLGAFVFGVHDDE
                     GPGVFEETVEWARKAKLDYAQFSINTPEPGARDYEHAVRNNLITDWNWEHYDAEHPVR
                     RFANITQEQMYQGLRNAYKWFYGLSSLKERMLGQFSDFLKGSSMCEDSSEFWWKRLKT
                     MALHTSVGLYLRGTANGYSRRGNVEAYQAGIDPEPNPLVLEQFGPGSPEGQYDARARM
                     RELRGELSRRTAQILKGRFPLTITGLQGQGPAISPSPSALPSE"
     gene            complement(14682..16007)
                     /locus_tag="PLY56_00050"
     CDS             complement(14682..16007)
                     /locus_tag="PLY56_00050"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ19905.1"
                     /translation="MWRRSSGSSAGDLPALWETAMTGQVTEFARALRDAGVRVSLAEA
                     LDACRALEHLSLERRADLRLALRCALIKDPADFPTFDLLFEKYFRCHWPRRKQRESRP
                     RTFRHEGAAPRPGGSQPGTRPAPPRPEPERKRDGKQPLNETDARKGERAPDQREERSE
                     EERWLAEWLADLEEGMGEARREGLRELRQRRSDLRRLDLTRRLEPEDARQVAEAVEKL
                     ARKLASRESLRRRRARRGKVDLKATLARSARTGGVPFHIERRRRTVAKTKLLLLCDVS
                     GSVWRVAAFLLRLIHHLQNQFARTHSLVFVDRPVDVSELLARYPFEDALARIRDIPDL
                     NLNAYSDFGNTFYELLDDYAPLLDRDTVLLVLGDARTNQFDPMEWALADLRQRIRRVV
                     WLNPEPPERWNTGDSVIRAYAPFCGAVLPCATLEDLEEAARLLLSDTRA"
     gene            complement(15973..16881)
                     /locus_tag="PLY56_00055"
     CDS             complement(15973..16881)
                     /locus_tag="PLY56_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ19906.1"
                     /translation="MSESQFTSIEEVIRRFEEQEYICPRDVAVTVYLAGQMGKPVLTE
                     GPAGVGKTELAKVWAQATGRELIRLQCYEGLDESKALYEWEYTKQLLYTQMLKEKIGE
                     ILAGAATLGEAVARLRTHESSFFSRDFLVPRPLLRAILADQPAVLLIDEVDRADQEFE
                     AFLLEVLSDFQVSVPELGTLTARHRPAVILTSNATRQLSEALRRRCLYLYMDYPSHEQ
                     EVKIVTRKVPGLDARLAEQAVQFVQKVRALPLRKAPSIGETLDWALVLVLLNARSLSR
                     QVLLDSLNALLKDREDVEKVLRELGR"
     gene            17133..>17359
                     /locus_tag="PLY56_00060"
     CDS             17133..>17359
                     /locus_tag="PLY56_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19907.1"
                     /translation="MVDARLCLLALLVLPAALPATAAPGTITLVDAGRSDYRIVVPAN
                     AIPSEQHAASELQSFLKQISGAELPIVTDAEP"
BASE COUNT         3015 a         5709 c         5695 g         2940 t
ORIGIN      
        1 aaagaggaaa agcgttccca ttgggtgggc atcggcatcg aggatgggcg cccttgtgat
       61 atcgggcatc tcatcactct ccgcggtcca tgcaggcgcg tccgctggcg ggacgttgcc
      121 ccagcatgcc ggcaccacgg gcaccagcgc gcccggctcg gtgatcgcga tggcggtgcc
      181 aaccccctca agctgggaac acacgcttgc gagaaattcc tccacagcgc cttccaggcc
      241 agccgaacgc gcacgctcgg ccaggcgatc cagcgtgggt tgaaccgcgg cttcggaagg
      301 cttggtgcca cacgcctgta tcagctcctg gaagcggtga acagcggtgt gcagcatcgg
      361 tccaatctga tggccgtgga tcggcttcac cagatagccg aacccgccgg aatcggccgc
      421 gcgctctatc atctctcggt cgccgagcgc cgtcaggaag accacaggca ccggctgtgg
      481 caggccgcga atcaggcgcg cggcgtcgag gcctcccata cccggcatgc gaatatccat
      541 gagcaccacg tcaggatgca gccgggacgc gcactccact gccgcctcgc cagaggccac
      601 gctggcacac acttcatagc cattggcgcg aagcacgtca cagaccgact cacgcaccat
      661 ctcctcatcg tcagctacca acacccgaaa acgtgggcta cccatccccc tctatcctct
      721 ccggcgcgcg ggccaacagg ctgttgccgt tccgctctcc tccatggccc ctggcgcagc
      781 gggcaccaag ggcacaggtc accccaaggg atcggtaccc tggagggagc ctccccaccc
      841 atccttcaac cggcatctag aggcccggtg ttctcacgca ctggggaaca ctctcccatc
      901 cggccggatg gttcgcgccc gctcaccgtt gtttgaaagg aactggcttt cgcctttacc
      961 gccattcgac gcaaccggta cgcagccctg ccatcagatt ccttcctact tcaaggcgct
     1021 ggcccgcagc ccatcttggc tagcgtgcat ccgggttaca gagacgcttc gcgaggagag
     1081 ccggaggcgg gggcgtggta gccttcaagg aatcgtgcca ggattccatc cagggctgca
     1141 tgagcagact gctcctttcc actacgccgg aggaggagta tcccccggca tcctaagcgt
     1201 acgtacgtac gattgcggat tgacaggcag gcgcccggca tgtaagatac atagtatcgg
     1261 caagggatgt ctgctagccg gccgttaggt gatggcagtt tcgagcggtg gcgacagact
     1321 gggccgaaag tggcccatca acttgcagtg ccccacgcaa gctctcgtcg gcccagccat
     1381 cacccccgtg agatcaatta cccggaaaaa ggaaggcagg aaaggccagg agatggagcc
     1441 cgtccacggt tatcagccga gcgcgcgatt gggagaggtc tcagggcttc atccaacgtg
     1501 cgcgttgtga gccctggtgt gcaggatgct gcgcctgggt acgtccaggg acgtatgatc
     1561 accatcgttc ttgcagatga tcatgccatc gtccgagatg gtctggtgcc gcttctagag
     1621 gctcagccag agttctcagt ggttgcggcg gtggagaatg gccgcgaggc tctggatgcc
     1681 atcgggcgtt ttcaaccaga tattgccatc atcgatatcc tcatgcccga ggtgggagga
     1741 attgatgtgg tgcggcaagc gtgcgagatc tccccaaaca cccgggcggt gatcctttcc
     1801 gcttactccg aagagcgata ccttctcgcg gcgttccgag cgggtgctcg ggcgttcgtg
     1861 cttaaggacg cgaggccacg cgacctgatc gcagcgatca cctcggttgt cgccggacaa
     1921 cgctatctca gccgtccgtt ggcagaggcg gttctcgatg cctatctcga tcaggtagag
     1981 cagagcggag cctgcgatcc ctacgagcag ctcaccacgc gcgagcggga ggtgctgcac
     2041 tggacggtcg aagggaagac agcgccccag attgcctcac ttctggagat cagcgtccgc
     2101 acggtggaga cacaccgcgc gaatgtgatg cgcaaactgg gaacacgcag caagagcgat
     2161 ctggtgcgct atgtggtcga gcgagccgag aggcaggctg atccacggat tcggggtggc
     2221 gcaaggcgag accccgcgcg gcacaccacc gcagccaatc gtcggtagga tgcacagacc
     2281 cgccaaagaa attgattaca gacctatggt gagctcccgc aaatccggca gccgcgggct
     2341 cttcccactg ccggatcgct cgccttgagg tttccgagat aaggagaccc cgatgcgggt
     2401 actgctggta gagaattgcg acgacgacgc tatcctgatc catgaattgc taaccaaagc
     2461 ccccgggggg ccgtacgata tcgattgggt cgagcgcctc tctgccgcgc aggaccactt
     2521 tcgggcggct gcttgtgacg tcgtactgct cagtctctgc ctcccagata gccaaggcct
     2581 cgcgactgta gagcgcatgc tacaggccgc acccgaggca cccatcgtcg tgatcaccac
     2641 cctcgaggat gaaacggtgg cgctttcggc ggtgcaacgc ggcgcgcaag actacctggt
     2701 gaaggaccag ctcaccccaa gcttcctagg gcgggtgctt cggtatgcca tcgaacgcaa
     2761 agaggccgag cgcgccctac gcgagagcga ggagcgcttt cgcgcgatca cggaaaacgc
     2821 atccgatatc gtggcggtgc tcagtcccga gggcaactgc ctcttcatca gcgcctctgt
     2881 cgagaatctg ctcggttgct ctgcgcacgc ggttcaggga acaaatgtgt tacagtgggt
     2941 gcacccacac gatcaggatc gcacccgttc ggctctccag gcggtcgcga atctctcccg
     3001 atccgcgcag cccgtaactc tgcgcgttcg aggcgcgagt ggctcctggc attggatcga
     3061 ggcgaaagca cgcaacctgc tgcagcatcc cgcggtacgc ggcatcgtgg ccaacgcgcg
     3121 tgatatcacc gagcaccacc ttgcccagga agcgatcaac gcccagctct ctcgtctaga
     3181 tctgctaaat gaactcaccc gtgcgattgc cgaccgtcag gatctggcgc gcatgttacc
     3241 catcctcctg gcccacctgg aggatagcat ggcgcttgat ggctctgcca tctacatcta
     3301 cgacgggcag gcagactccc tttcgctcgc ggcctccagc gggctgctgg aaggcgatcc
     3361 ccatcaaggc gaccatggcc caccctcgag actgtcaaca gaaggtctcc gctcttgtct
     3421 cacccgccaa tcgacttacg tgcgcgacta tgccgagtct ccagagacga tccccttgct
     3481 ggccaacgcc ggcctgcgca gcggcgtggc cctcccgctc catgcaggcg gcatgctcta
     3541 cggtctcctc gtgacggcaa ggtgccaggc ccatggcttt accagcgacg agattgaatt
     3601 cctgcgccgc cttggggagc acatcgccct ggccatccgc caggataccc tgctgtggga
     3661 cctccattct gcctacaagg agctgcagcg cacccagcag agcgccctcg agcaagagcg
     3721 cctgcgcatc ctgggccaga tggcaagcgg aatcgcccac gacatcaaca acgccctgat
     3781 gccgatcacc acgtacgccg agctcctggc gatgcgagtg cccgatctcg atgagcatgc
     3841 gcaaagatac ctgaagtcca tcctcatggc cgccgatgat atcaagaaga cggttggtcg
     3901 catgcgcgag ctgtaccgca agcgtgatgg gcgcgatcag gtgcgctcca tccacatcgc
     3961 gcgcctgatg cgtgacgtgg ttgacctgac tcggcctcgc tggcacgacc ttccgcaaca
     4021 gcagggcgtc accattgccg tgaagataga gaccgatggc gcgcctacct acgtcacggc
     4081 cgtggagagc gagctgcggg aggccctcgc caacctgatc ggaaacgccg tcgatgcaat
     4141 gcccatcggc ggctccatca ccctgcgggc cagaccgtgg tcgcggtcgc tactggccat
     4201 cgaagtatgc gacaccgggt atggcatgga cgcggagacg ctgcgccagt gtctggagcc
     4261 cttcttcacc gccaagggcc agagcggcac cgggctgggc ctgagcatgg ttcagggcat
     4321 cgtccagggc catggaggaa ccatagagat cgaaagccag ccgggcaagg gcacgacggt
     4381 gcgcttgctt ctcccaatgg acgggccagc ggctgccagg cgggagctcc cctcttctgg
     4441 ctgctgccag tcgcgaccac tacgcgtcct ctgcgtcgat gatgacgacc gcgtgcgtgc
     4501 cgccctcatg gatatgctgg aggcggatgg gcacatagtc gtgggagccc cggggggcga
     4561 ggcggggata cagcagtttc gccacgcgct acttcacggc atgccgttcg atatcgtcct
     4621 caccgaccta ggaatgccac acattggcgg gcgcatggtc gccctcgcca tcaaggccga
     4681 gagcccggag acgcctgtcg tgttgctcac cggctggggt actttcatgg agcccggggt
     4741 gtttcccgag gggatcgatg ccatcctggg gaagccgccg gagctagcgt cgatccgcaa
     4801 ggtgttcgcg aaactcaccc agcggtaatc catcctctcg ctcgccgcgc ggggctcgta
     4861 cgtagctcag gcgggcgctt gggcccgcct ggcacaacgt ccgatcgatt ggtgaaagcg
     4921 cctacttcgt ggggttccac atctcgcgct cggtcttctt caggaacttc acgtgaccat
     4981 cggcaaagag ccaattcgag ccgctcgagt gcctgcggtc gatcaggccc actccgtcgg
     5041 ctgcgtaggc atccgcgtga tccacgctcc accactcctt gccgcctccg gtcgaggtgc
     5101 tccgcgtctc tccaatgagg atcaggttcg aaaccgtctg aatctgcgag agcgagcagg
     5161 atgtgccagc gccgcccatc ggccggatgc cgctaccgcc gccgtgcccg ttcgcggcgt
     5221 aggagaactt caaggggttg gccgttcccg ccgggcgcag gttggcatag actccgtccg
     5281 agtcgctggg acaagccatc acgttgtagt tcttaacgta gggctgaagg agatcctccc
     5341 accaagcgag ctgagtcttc gccgagtcca ggtagaggta ggatgggggc aacacctcat
     5401 cgtagtcctg ggcatacatc aggatgccgg tgccaatctg cttcaggttg gactggcaat
     5461 tcgacttccg ggcgttctcg cgcgcctgcg cgaacaccgg gaacaagatg gccgccagta
     5521 tagcaatgat cgctataacg acaagcagct caatgagcgt gaaaccggat tgccggtccc
     5581 ttcgccactt catgttcttt ctatcctctc atacaggtga tgatgcacaa cgcgtgctgg
     5641 ccatgtctag agttcgacgc ccccggagca tccccttctt gggagcagtc ttgggatgca
     5701 cgggggaggc cactctgctc tcgcgcacgt gcgcgccgcg ctcccccgat tcatcccaac
     5761 ggctggtaaa gcctgcgctc ctcgacggcc gaaagccgcc gaagcgcgga gtccggccgg
     5821 ctcccggtca caagggacac aagatgggag aaccatcagg cggacgtccc gtcacttccc
     5881 ccattcaccg cccccaggag gcgctgggca ggatctccca gctctcgccc gggcgggcct
     5941 caaaaccggt cccctccgtc ttcgtataga cgcgtgtctc gccgtccacg acagccacac
     6001 gccggagggg gtaggcccgg cgcatcccat ccgcctcgat aaagagcgtc tcgccagggc
     6061 gcacgccctc cttgggaagc tcgccgtcca ggacgaacca tgcctctccc cgcgcgctgc
     6121 ctgtccccgc gacccgacct gcgtagcggc cgcgggcgat ggtcacgctt gccgcgccga
     6181 gcgaaagccg ggttcccccg aagagcgcgg tcgtgacagg cgcctgccca tccgccagaa
     6241 ccgcagctgc taggccatcc gtcaccagct cgccgagcgg cgtggtcacg cgaagtacgc
     6301 gcggctggag ctgcgagagc acgatatcag ttccaagcgc cgtctccacc gcgagggcaa
     6361 tggcgtccga gggcgtatct gccgggaggg gcagcacgcg aatgccacgc accgggagct
     6421 tccctttcgg agcgccggcg aagagcgtca cgaacgtgac cggcttcggg ccgcgcgagg
     6481 agcgcacgac atagggaagc acggcgccac ggtcgcggtt gcggtggtcg cgctggcccc
     6541 aaccgttgcc caggcgcacg gtttccccag gcgcgcccgg cgcgagcgcc gcgaaagtgt
     6601 aatccccctc cagcgaccag gtgatctgcc aggggtttcc tccctcggcg cgctgcacat
     6661 cggtcaacgg gagcacgctg gttggtaggg tgaaggtgac ctcataatgc tgccagccct
     6721 catcaaccgg ggcgttgatg atgttcacgt agctgcggtc gtcaggcgat ttcgggtcat
     6781 tgggccaata gaccgcctga actctcacct gagggacgtt gccgcgcagg cggaagcgcg
     6841 ccttataggt ggcgcccatg gagcccgata gcgccccttc cccccgatag ccatccgact
     6901 tgccagcaag gagggcgacg ttcatccggc cattctcgtg cggcttgagc gcgcgcacgc
     6961 gcatgcaccg cccgcccgcc tcgtccgagg gcgcgatgcc ccaatcgccc gttccatctc
     7021 ccgagtagta gccccatccg gccggctggc ttcccacctc gcccaggatc ggtgcgatca
     7081 ggttagggcc ttcctgttcg ccaggcatca cccggcggat ctccacatcg gccacgtaca
     7141 gctcgcccac gcgcgccagc gggaagcgca gggcaaagcg cacctcgcgc tccacctggc
     7201 catccggcgc cagcgcgagg cccgtcacct gatagtcacc gcccggtcca tggaagacgt
     7261 aatcgcgctc ggtgccaccc tcgacgcgga agagatccac caggtaggac cccgcatccc
     7321 cgtgatccac ctggatgcac gtgcgccggt aggtggatgc agcatcatag gcccgagagg
     7381 aggcttccat taccttcacg cgcggcgtac agccgaagag atgaaagctg ccaccgcggt
     7441 cgcgcgtctt ctgatccttc cgatcgacca ccaccaggtt atgcgcgaag gtgcgccgcg
     7501 tctggtagga atcggggtga tcccacaggt agcccaggtc cgagagaagc tcgtgcccgt
     7561 ccttccagta gtagagatcc aggctgtcca tgtggtggtg cccgccccaa tcactggcgt
     7621 tgaggagggc caggctcttg cggccattct cgccggtgcg caggttgccc tgggaaagga
     7681 aggggaagac gatatcgggc aaggagagcg gcggaacgga acgtgcttcc aggccgggct
     7741 cgcggtagaa gatcgctgcc tgcggcgacc caggcgcttc atcaccgctc agctccttca
     7801 ggagggcaat gtgttcgggc gtcggatagc cgagggcgag cagctcggcg aaagatgcat
     7861 tgatccgggt tgtccggtaa gaatcagcgg aaggaggatg aaagagatcg ccctgcagcg
     7921 tccagaccag gccctgccag cagtcgccat agcgcgtatc gcggcaggca tcgaagttgt
     7981 cgaggcgggt gccatcgggg ccgcggtagc cgggcggatc ggagtagccg cggaacatca
     8041 gcgcgaagtt gcgaacgccg cccatcgtca tcatcgcgta ggcggccgat tcgcttgtgc
     8101 ctccatcggg gaggaaccac tcctccacag tccggcggaa gccatccagg ccgaaatgga
     8161 ccaggcccgg gtggccaacg cacatcccca ccatcgccgc gccggcgcga ttgcccaccg
     8221 acttgttgtt gatcgctgga tcgcacgcgg ccagatatgc cccttccagc aacacgtcgc
     8281 gctcgatgcg cacccggtcc tcgtccgaga agatcggagt gtcgccctct ttcgccatgc
     8341 aggtgaggtc atacgcttcc gtgatggtgc agatcaggcc gccatccatc ccggaagtgc
     8401 cgagccggct ggcagaccag tagccggtgt gcagcttccg gtctggcttg ttcgggggca
     8461 ccaccagctc atcggttggc agattgctga tctgccgggc ggccacatgg ggatcgcagt
     8521 cggcatattc gccgtaggcg tatccggcgc gcaccaagta gcgcggaagc acccccgcca
     8581 ttcgaaggag gatggcgcgt gccgcccggg catagcgcgc atcctgcgtc agcgcgtagg
     8641 catgggcaag cgtgcgcagg tgggaagtga tctggcttac tttccgggcg cgaatgatgc
     8701 cggtgagact ggggcgcgcg ttgtaaccga agcaccggaa cggcggtccc tggccaacaa
     8761 aggtgaactt ctgctcgggg tcccacttgc tttgcaagac gatatcctcg gggaactcct
     8821 cgttcgggaa gaccgtcttg cagatgttgc aggtgagctg gtccggggtc tgctcattcc
     8881 aactccactg gccattggga tgccagggaa ggcccttcgc ccggcaggca ggacaggggc
     8941 ctacacaccc cggggtagtg cgctcgagca tgcggacgag atactctggg gtgattcgtg
     9001 gcaccagggt atcggcgctc gcgcgcagcc tatccacgta ggcttgtgcc caaggatatc
     9061 gtttcacatt ctcctgcgcg cgctccaggt ctgcctgctt gatcgagctt gccgggtgct
     9121 gcactcccgc gcgatagcgc tcccaatccg accaatccgt cgggggtttc gcttcagcgc
     9181 ccatagcgcc tcctccagac agcgccatac agatgagtgc tgcagccgct gccagtcgca
     9241 gtgtggtagc catactcacg ttcgccctcc gaggtcggcg ctcgtgccgc cccctcttgc
     9301 ctgccagtgt agcatggcgg gcgggagacg tcaagagaag cgtagggagc ctcaccgttc
     9361 cgggctcgga agcgcatcgt cgccctggcc gcgcatccta ccccccgact acccgcccca
     9421 tccctagcct cggaccgcac gccggcccca gggaaagcga gcgaccgcgt gccggtcgcg
     9481 gtgaatcctg aagggggccg gcggtccggc ttctccgcgg ggaggttgcg tgctcctgct
     9541 catcgaacag gtgagtggtt tgccgggcga ttctttcacc ggcgcgcgac ggcgtcatag
     9601 ccacggttcg cgtgtttgcg aggggacaga tgcagtatac agaagggcag attggacgcg
     9661 tctttgccat tcgtctggag catggagatc cgatgccgca ggcgctggag gatttcgccg
     9721 cggagaaggg cgtgcgatcc gggctggtga tcctggtcgg cggcgtcgag gatggcagcc
     9781 ggctggtagt cggaccagaa gacggagaga gcctgccgcc cgtgccccag gtaggcacgc
     9841 tgaagggggt acacgaggcg gctggcgtgg gcacgctttt cccggatgag agtggcaagc
     9901 cggtgctcca cctgcacgcg gcgtgcggcc ggggcgagaa gacggtcacc gggtgcattc
     9961 gcgccggcat cgtcacctgg cacgttctgg aggtgctcct ggtagagctc actgggctgg
    10021 atgccgtgcg tgttgccgac cccgtgacca agttccagct gttgcgctgc ggcgaagggt
    10081 ctcgcgagca ggcgtaagag cgcgcatccc tgatgcggtg acgcgtctcg tttgggaaga
    10141 aggcgatcga tgctcgattt ggtgtttgtt ggcggggaag tgatcgatgg cacggggcga
    10201 ccgggtatcc gggctgatct cggcgtgcgc gacggccgca tcgccgctat cggcgacctg
    10261 agcggcgtgg agtgccgcga gcgcgtggat gcccggggaa aggtggtttg cccaggattc
    10321 atcgatatgc acacccactc cgatctcacg cttctggtta acccgcgcgc ggaaagcatg
    10381 gtgcaccagg gcgtgacgac gctcgtggtg ggcaactgcg gcatgggggt ggcacccgta
    10441 cgcccggagc tgcgcgggcc gctgcgccag gcgttcgcct tcctcaccgg cgacgtggag
    10501 tggacctggg agagcttcgc cagctacctt gattgcctcg ccgccgcccg gccctcggta
    10561 aacgtcggcg ccctggttag ccacggtgcc gtgcgcgccc acgtgatgag ctttgatgag
    10621 cgagaggccc tcccggagga acggagcgcc atcgccgcgg agatccggca ggcaatggcc
    10681 gagggcgccc tgggcgtctc caccgggctc atctacaacc cgagcatgtt cgccgatacc
    10741 gaggagctgg tcgccgtatc gcgcgcggcg gccgagggga atggcgtcta caccacgcac
    10801 atgcgccatg agggcgtgca gctcatggaa gcggtggagg aggcgctgga ggttgcccgg
    10861 cggagcggcg cgcgcaccga gatctgccac ctgaaggcat cggggaaggc cgcttggggc
    10921 cgggccgccg aggcagcgga gcggattcgc caggcgcgtg aggaggggaa cgacatcgcc
    10981 ttcgacctct acccgtacac cgcggggagc acgatgctct cgacgctctt gccccagtgg
    11041 gcgctccgcg gcaccaccga tgagatcgtc gcccgcctcc gcgacccggc ggcgatcgcc
    11101 cgcctcttgc acgagctgcg ccacgggggc tcgggctacc acagcggcct cggcgcgccg
    11161 ccttacgagt gggagcgggt gctgatcacc tccgtcggaa gcgagatgaa ccgtggcttc
    11221 gagggcaagg agatgccgga agtcgcccgg atgatgggtg ccacgcccga ggaggcgttc
    11281 ctgcgtctcc tggtggatga ccacctccag gtgaacatgg taatccactc gcagtgcgag
    11341 gaggatatgc gcgggctcct caaacacccg ctcgctttgg tgatcacgga cggactggcg
    11401 ttcgcgccgt atggccccct ggggcgcggc cggccacacc cgcggagtta cggcaccttc
    11461 ccgcgattcc tcgggcgcta cgtgcgcgat gagggcctcc actcgctcga agaggggatc
    11521 cggcgtgtca cctcgggccc ggccgcgcgg atggggatca ccgatcgcgg tatcctccgc
    11581 gagggcctgg ctgccgatat cgtcgttttc gatcccgccg cgattcgcga cacggcgacc
    11641 tacaccgagc cgcaccagta ccccgagggg atcgacctgg tggtggtcaa cggcgagatc
    11701 gtggtgcgcg atggcgagca caccggggct ggccccggcc aggtgctccg caagaagtga
    11761 gcgcgagggg ccggcggcag ccagaaggcc ggcccctctt cacacagcct acaagccggg
    11821 ggtggctacc cctcatccac cgcgcggatc cacgcctgag cgatcagggc atggccggcc
    11881 tgggttggat gcacgccgtc ggctgcccag aaggcggccg ggcgctgcgc cgccgccgcg
    11941 gcaaagaggc catccagcgg cacgtagagc gtcccgtaat ccgcggcgac gcggcgaacc
    12001 gcggcgatgc gcgggtcgag atcctcgcgc caggcaacgc ggtcgggcgg ggttggaagc
    12061 acaaatggct ccagcaagat gacgcgggcg gaggtctctg ccttcacgcg gtcgaggaga
    12121 cgcctgtagc acgcctcgaa ctcctcgatc ggcgagagct tgccgctatc gtagcgccgc
    12181 caggtgtcgt tgatcccgat caggatggag acccaatccg ggtgcaggtc gatgcagtcg
    12241 cgcgtccagc gctcttccag gtcgtagacg cggttgccgc tgacgccacg gttcaggaag
    12301 gtgagctcgc gcccgggata gcgggcggag agccacgcgg cgatcaggtt ggcatagccg
    12361 gatccgagaa gcgcgtcgtt ctcgcggctg cgcccggcat ccgtgatgct atcgccctgg
    12421 aagaggatgc gggcgcccgg ctgaagcaga agatggccca tctcatactc cctgtcattc
    12481 cccctgggtt cgaatcgccc cagggcgggc gggatgatcc cgcccgctgg ggtattcgat
    12541 gtggagccgt ggtcccctcc cccagattcc cccgcacgga cagctcactc gctcgggagg
    12601 gccgacgggc tgggcgaaat cgccgggccc tggccctgga ggccggtgat ggtgagcggg
    12661 aagcgcccct tcagaatctg agccgtgcgc cgcgacagct cgccgcgaag ctccctcatc
    12721 cgggcacggg cgtcgtactg cccctccggg cttccggggc caaactgttc cagcaccagg
    12781 gggttcggct ccgggtcgat gcccgcctga tacgcctcca cgttgccgcg ccggctgtag
    12841 ccgttggcgg tgccgcgcag gtagaggcca acgctggtat gaagcgccat tgttttcagg
    12901 cgcttccacc aaaactcgct gctatcctcg cacatcgagc tccccttgag gaaatcgcta
    12961 aactgcccca gcatccgctc cttcaaagaa gagagcccgt aaaaccactt gtaggcgttg
    13021 cgcaggccct gatacatctg ctcctgagtg atattcgcga accgccggac ggggtgctcg
    13081 gcatcatagt gctcccagtt ccagtcggtg atcaggttgt tgcgcaccgc gtgctcatag
    13141 tcgcgcgcgc ccggctccgg ggtgttgatc gagaactggg cgtagtcgag tttcgccttc
    13201 cttgcccact ccaccgtctc ctcgaacacc ccgggcccct cgtcgtcgtg aacgccaaag
    13261 acgaaggcgc caagaacacc catcccatgg tcatggatgc agcggatcag atcgatgtag
    13321 ttctgaagcg aggtggactt cgccagcttg gcggtgatcg actcgatccc catgtagatc
    13381 ccgacgcagc aggatcggga gaagaggggc accaactcgc gccaattgcg gaaaagggtg
    13441 agcgcggtca actcggtcac ccaagggatg cggtggtggc ggagcatctg gcagacgcgc
    13501 ttcgcgtgct ctccccgggg gttcggcagg aagttatcgt cgataatcgc tgtgagccgg
    13561 cgtggaagct gggcaatctc cttctccagg tcttcatagt ccgtatgtcg ctgcttcatg
    13621 ccatattcat aggtcacgga gcagaaactg cagtcgaagg ggcagcccac ggaggtctgc
    13681 agcgtgttgg ggagcaggta gcgtttggga tcgtaccagg agcggtcggg gatcgggcgg
    13741 cgctccagcg tctccatgcg cgcgttgggg gcatgctcaa agacgcgccg ctccgagccc
    13801 tcctcttcaa tttgctcgat gcaccactcg agggcggcct cgagcagcac cgggggcgtc
    13861 tcgccacgca ccacaccatc ggcgtaatgg atcgcctcca ggggaagcgc cgtggcgtgg
    13921 atcccgccga tgagcgagcg gacacgccgg gattgccagc tcagcgtctc gcgggcttcc
    13981 gtcaggatcg cgtaggcgcg ccgcgcggcc gtagtgagcg ccgagaccgc gacgatatcg
    14041 ggctgttgcg cgcgcacgtc gtagggcccc atcgtgtgct catcatgaca gcgtgcgatc
    14101 aagtcgtaac cgcgcagaga gggcggggtc atcgcgcgca ggagggagat actcatgggg
    14161 ggaagcggca tcttgcgatt ccccatctcc tcacggacga tacgatccgg tatgacgtaa
    14221 tcgatgatga tcttcgccac cgcaccactc ctcaaaatat gacaaacaac aagcaccccc
    14281 aatggaacga aagggaagtg gagaggggtg ccgcttcggg ggtgcccaca cccgctgcca
    14341 tcccccgggg gtgctactgc caggtgttga ttccagtata ggcccgcgtt cgtgagcgtg
    14401 tcaagcaaca gccattcaga tgggggattt cgagataagg agatggatgg agagggccgc
    14461 cagcgggcgc gggctcgaag ctccgcgctg cttcccggca aagccctttc ggggctcccc
    14521 ctcccagccg ccaccatccc cggttgccca cgcgtgcagc ctggcccagc ccggcagggc
    14581 ttcgctcaca ggcagcacgg tgatttatcg ccgcgcgccg ggttgcgcgc caccttcgat
    14641 tcggattcct tatctcggaa tcccagccat ccagatggga atcacgcccg ggtgtcactc
    14701 aggaggaggc gggcggcctc ttccagatcc tccagggtgg cgcaagggag gaccgcgccg
    14761 cagaaggggg cgtaggcgcg gatcaccgag tctcccgtat tccagcgctc cgggggctcc
    14821 gggttgagcc ataccacgcg ccggatccgc tggcggaggt cggcgagggc ccactccatc
    14881 ggatcgaact ggttcgtgcg cgcgtcgccc agcaccagga ggacggtgtc gcggtccaac
    14941 agcggcgcgt aatcatccag gagctcgtag aaggtgttgc cgaagtcgct gtaggcgttc
    15001 aggttgagat ccgggatgtc gcggatccgg gcgagggcgt cttcgaacgg gtagcgcgcc
    15061 aggagctcgc tcacatccac cgggcggtcg acgaagacga gcgaatgggt gcgcgcgaac
    15121 tggttttgca ggtggtgaat caggcgcagg agaaaggcgg ccacgcgcca gaccgacccg
    15181 gagacgtcac acaggaggag gagtttcgtc ttcgccaccg tcctgcgccg gcgctcgatg
    15241 tggaagggca ccccacccgt gcgtgcgctg cgcgccaggg tcgccttcag gtccaccttc
    15301 cctcggcgtg cccggcgccg gcgcagcgac tcgcgcgagg cgagttttcg cgccagcttc
    15361 tcgaccgctt cggccacctg ccgcgcatcc tcaggctcca gccggcgggt gagatccagg
    15421 cggcgcaggt cgctccggcg ctgccggagc tcgcgaagcc cctcccggcg cgcttccccc
    15481 atcccctcct cgagatcggc cagccattcc gccagccagc gctcctcttc cgatcgctcc
    15541 tcccgctggt ctggcgcgcg ctcccccttc cgggcatcgg tctcgttgag aggctgcttc
    15601 ccatccctct ttcgctccgg ctcgggcctg ggcggggcgg gcctcgtgcc gggctgactc
    15661 cctcccgggc gcggcgccgc gccttcgtgg cggaacgtgc gcgggcgcga ctcgcgctgc
    15721 ttccgccgtg gccagtggca ccggaagtac ttctcgaaaa ggaggtcgaa ggtggggaag
    15781 tccgcgggat ccttgatcag ggcgcagcgg agcgccaagc ggaggtcggc tcgccgctcg
    15841 agcgagagat gctccagcgc ccggcaggcg tcgagtgcct ccgcgagcga gacacgtacg
    15901 ccggcgtcgc ggagagcgcg cgcgaactcg gtcacctggc cggtcatcgc cgtctcccag
    15961 agcgccggga ggtcaccggc cgagctcccg gaggaccttc tccacatcct cccggtcttt
    16021 caggagcgcg ttgagcgagt cgagcagcac ctggcgcgaa agcgagcggg cattcaggag
    16081 gaccaggacg agcgcccaat cgagcgtttc gccgatgctc ggggctttgc gcagcggcag
    16141 ggcacgcact ttctgcacga actgcaccgc ctgctcggcc agccgggcat ccagcccggg
    16201 taccttgcgc gtgacgatct tcacctcctg ctcgtgggag ggatagtcca tatagaggta
    16261 aagacagcgg cggcgcaacg cctcggagag ttggcgcgtg gcgttgctag tgaggatcac
    16321 ggcgggccgg tgccgcgcgg tgagcgtgcc caactccggc acgctcacct ggaaatcgct
    16381 cagcacctcc aggaggaacg cctcgaactc ctggtcggcg cggtccacct cgtcgatgag
    16441 gaggacggcg ggctggtcgg cgaggatcgc gcggaggagg gggcggggca ccaggaagtc
    16501 gcgcgagaaa aagctgctct cgtgcgtgcg gagtcgggcc accgcctcac ccagcgtcgc
    16561 cgcgccggcc aggatctcgc cgatcttctc cttgagcatc tgggtgtaga gaagctgctt
    16621 ggtatactcc cattcgtaga gcgccttgct ctcatccagg ccctcgtagc actggagccg
    16681 gatcagctcg cgcccggtcg cctgcgccca cactttggcg agctcggtct tgccgacgcc
    16741 ggccggaccc tcggtcagga ccggcttgcc catctgcccg gccaggtaga cggtaacggc
    16801 tacgtcgcgc gggcagatgt actcctgctc ctcgaaccgc cgaatgacct cctcgatgct
    16861 ggtgaactgg ctctcagaca tctttcgggg aatccccgcc ctctccaaag atcggatcgc
    16921 ggccagccgc gatccctccg cgcccggctc ccgctaagga gtttcgggag gccgcccccg
    16981 gttcctgtcg tcgcgagcgg cgtcgcgccc acgggaggag atcccgggag ggcgcgtaac
    17041 tactgacaaa acgagcggcg gccgcgtccg cgagggcacg cggcgctgaa cggcactggg
    17101 ctcgggggat atccccggag ggaggagtca ccatggttga tgcacgtctc tgtctgctcg
    17161 cgctgttggt tctcccggcc gcgctgccgg ccacagcggc gcccggcacg atcacgctcg
    17221 tggacgcggg acgctcggat taccgcatcg tcgtgccggc gaacgccatt ccctcggagc
    17281 agcacgcggc ttcggagctg caatcgttcc tcaagcagat ctccggggcc gagctgccga
    17341 tcgtgaccga cgccgagcc
//
LOCUS       DAOSCV010000002        17099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_2311_length_17099_cov_3.325452, whole genome shotgun sequence.
ACCESSION   DAOSCV010000002 DAOSCV010000000
VERSION     DAOSCV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2311_length_17099_cov_3.325452"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLY56_00065"
     CDS             complement(<1..988)
                     /locus_tag="PLY56_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19908.1"
                     /translation="MTMVRDGVSIGPFLLGSLVLHTFGFAAVAHLQGPPRKPPSARVI
                     EVAVIQPEKAPAPKPAPAKTTLASDKPPRPSRSALPRPASPARRVSKTPIATRTSPRP
                     KPSAPAARKQDAVSETVSRAAEPSPVPAGPRAVQMARRALPEAEFPEWRPRPNPPGSG
                     TPAGPGSNRQEEAPGGGMAGPRRLSRARPEVSFPGDPLGETQALPGGTGRAAREPLPL
                     GLPIGRRDAAGEPEPGLVSRQVGGGAPTPGSGDDPGGGSRAARAPETVTFRFGAGGMR
                     LPKATPRIGGGGGTASVLATGPSTREADEVPVSPRPGVGPGQGGGAGAGIGGG"
     gene            complement(1031..1468)
                     /locus_tag="PLY56_00070"
     CDS             complement(1031..1468)
                     /locus_tag="PLY56_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ19909.1"
                     /translation="MRVPNNDGKMMAEINIIPLVDISLVLLIIFMVTTAFVKEAGLNM
                     KLPKAATTEAAPEKQQDISIAIAGDQKLYFDGRQTTPNQLRARLRMRASKSTEDRVII
                     KGDQHVPYGKIMMVLDEVKLAGFTHIALATEPIVEAPPADERR"
     gene            complement(1455..2126)
                     /locus_tag="PLY56_00075"
     CDS             complement(1455..2126)
                     /locus_tag="PLY56_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ19910.1"
                     /translation="MTHKEGCLKTMFNLGVLSEGGPLVLVYMLPLVVYSILSVAVILE
                     RTYTLRRLRRIEEEEYPRLREGVRNGTRDEVLQDLEANPAPVAPVLRAGAERSEFGAE
                     QVKEGIAQALALQTAGFSRYLGILATVGSTAPFIGLFGTVLGILRAFNKIAQQGFGGP
                     SVVAGGIAEALIATAFGLGVAIPAVIAYNIFVGKVNDLTLLVGSHASDLTPYIMQRET
                     IDAGA"
     gene            complement(2116..4974)
                     /locus_tag="PLY56_00080"
     CDS             complement(2116..4974)
                     /locus_tag="PLY56_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ19911.1"
                     /translation="MLLFRVFNRQPGTPLLPSRPSVVAPSARGGTARRVWALAAVLAA
                     LSLLPALGAVDPKQQLDFAKGLYERKLYSLAVPEFEKFLKSHPKHAGAATASYLLAHC
                     YRGSGDWVKAAAAYEAALRTYPKDPQADGALFSLGQCYLRLEQLDKAAATFTRQIETS
                     KDPEALAQARYWLGEAEYQRRRYPQAEAAYRSLVQKHPTHKHVPYAYLSIGACAAERG
                     DPQAAIAALRVILERYPQFEAIDESRVRLGDALRALGRHREAREAYAGVSTRAPALRL
                     AAMLGSAYAALDAKDYRAVLAATAECATLSETASRERAETDILAAGARYGLKEYPAAL
                     EAYERVAESRHADLAEEALYWTGNTLRAAGRPDEAIARYTALLTRFPKGAFAARARLR
                     IGDCHADAGRPGDAAAAYRAVLEAHPNTDTAREAQAAIADLLKQAGPGGEETSQLLRE
                     VGKLPPGSIAADAQIQLAQKEFAAERYAEAAALAERVLAGKPSPEAAEQALYLLGAAC
                     LQTRDAAGAVKAYTQLLEQHPKGKLVEQALLELAWAHLDLKQYPEAARAARRLTDRNP
                     QGPIRVPAFLTLAEAATMAGQYAEAEEAANKALASNPGPTETVSALYALAWASHSQKD
                     YRTAAQRWQRALEKARALPESPMVPRIAYYLGYALTQNKAYREAIPALELAAEKGPFA
                     ERARYDLAWNYAELKEHDRAAAEFERLAADYPEGRYAAEALFLAGEQYFARKEYQAAV
                     QRYQRAEARKPEGELGYQIAMRLGAALYYTPDYPGAAAAFARAAGLAPGAKERAEASF
                     WAGDSHAKAEQWREARPCFERALQSDPDGPLAAQVRLGLVRCLLATSEAAAAADLADE
                     GLKAAQGDAAAELQLRKGEALQAQGKHADAATEFLKVAVLYPQSEWAPKAQYQAGQAY
                     ENAKEKTRAIAVYRALIEQYPQSQAAEQARARLAALDP"
     gene            complement(5323..6495)
                     /locus_tag="PLY56_00085"
     CDS             complement(5323..6495)
                     /locus_tag="PLY56_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOJ19912.1"
                     /translation="MRIRRNRLYRDTRATQKVLWAMLAMGCLLLAAPASPAAAAAPEE
                     YLRAYLVSKQQLGNAANVVEVRGLIQGRMVSAQTVTWILRAESDDAPGYQITSPVDYP
                     VFIVGSRVRVIGRAAAADASVLELIAMVDESIAAAWEKAHAPKPQPRPDSTQTVPSPA
                     QNTTFPSQPSPATQPPGETAEPPQPSPAPAASPVSAPPPPRWKEPATASRPPASKTRP
                     NQQTQAMTSRGWSGYLDAYKRAIRYFNPRLSDAQQEAIARSILAYSAHFGIDARLVVA
                     VIAVESGFKPTATSHKGAMGLGQLMPGTAAGLGVRDAYDPVENIYGAVKLLRSHLKQH
                     ADKPFWEQLKLALASYNAGAGAVRKYGGVPPYRETQRYVEKVARLYAHLCGQSPAK"
     gene            complement(6595..6930)
                     /locus_tag="PLY56_00090"
     CDS             complement(6595..6930)
                     /locus_tag="PLY56_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HOJ19913.1"
                     /translation="MRLSQVIDELGLRALTPVQDREVTGGYVSDLLSDVLANGREANI
                     WITIQAHRNVAAVASLKDFTAVILAGNREPQDDLLEVASQEGVCLLSTPMTSYEIAGR
                     LYALGVPPS"
     gene            complement(6931..7875)
                     /locus_tag="PLY56_00095"
     CDS             complement(6931..7875)
                     /locus_tag="PLY56_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ19914.1"
                     /translation="MSVINHTNGFSVAHELIYTLKVRDAMSRDVVTVTPDNSLREVQA
                     ILRERRISGTPVLEDGRLVGIVSLEDIISALDHGHIEERVGDWMTTNVVTIQDNMPLH
                     RAVEYFRRYRFGRIPVLDAGGCLCGILTQADIVVRLMEELNHIAEEAARREIALLQRA
                     GTPITQGQEVTMEFPVNAGDFDNAGIASARIKAALKERGIDIKVARRAAIATYEAETN
                     IIIHSIGGAITARIGAESITIQAVDWGPGIEDVEMALRPGFSTASELVRVMGFGAGIG
                     LPNIQKCADRFHIESKPGSGTRLEVEIDLHPLVSEGGE"
     gene            complement(7872..8207)
                     /locus_tag="PLY56_00100"
     CDS             complement(7872..8207)
                     /locus_tag="PLY56_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19915.1"
                     /translation="MLLREVAQALEAEEVVRAEDPDIEVDSAAAADLLSDILATGKEQ
                     FVILTGLVSPQVIRTAQVVGALAVFLVRGKHPTQEIIGLAQAHGIPLYTTSLPLFESC
                     VRLAALAKP"
     gene            complement(8540..8923)
                     /locus_tag="PLY56_00105"
     CDS             complement(8540..8923)
                     /locus_tag="PLY56_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ19916.1"
                     /translation="MIVELRDETGVAVIEPLGRLDARTAGTFRQRLATLVEEGKSWVV
                     ADLSRVDFVDSVGLAALVSGLKMARSSAGDLRLAGLQGPVRVIFEITRLHRAFDIYDT
                     LPEAVHSFLHATEESPPIPRRVLPS"
     gene            complement(8985..9332)
                     /locus_tag="PLY56_00110"
     CDS             complement(8985..9332)
                     /locus_tag="PLY56_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ19917.1"
                     /translation="MRPVQLLRFSPHGAVGKLAERLGVSRLPALYNVMRGDISLVGIK
                     PMTEEEAASVSEDWLRHVGQAPAGFTGLWRIQGGDDLPLEETLAVDAYYAASKSLWND
                     LRILLWAATRRKR"
     gene            complement(9410..10570)
                     /locus_tag="PLY56_00115"
     CDS             complement(9410..10570)
                     /locus_tag="PLY56_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOJ19918.1"
                     /translation="MKAMILAAGVGSRLSPITGDTPKPMLPVANKPVMEHTIEMLVRA
                     GITEIKANLHHLPHLVEQHFGNGRRWGANLSYSLESTLLGTAGAVKRVQRFFDDTFVV
                     LVGDSLWDIDVAAVVAYHKACNAAVTVVVKPMPQEMVGRFGVARVDDDGRILQFQEKP
                     RPEEACGNLVSTGFYVLEPSVLDLIPQAEFFDFARDLFPKLLERGLPFYAYCYEGPWS
                     DIGAFSEYYQVHQEILSGSYSRLHLPAREVAPGIRLGHHTRIHPHANIQPPVMIGDYC
                     RIARGAVVGPNTVVGPHSVVDEEARVAHSVVGPTTASVGSWSWRGASLMGGGSCDLIA
                     APAPTSKTTCSCRISGDGRGGRSGRERRTSGSARSRSSVACRSFLGWASSRG"
     gene            complement(10567..11715)
                     /locus_tag="PLY56_00120"
     CDS             complement(10567..11715)
                     /locus_tag="PLY56_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOJ19919.1"
                     /translation="MRRLTVVLGVPVDEVTQAEALDRIEEFVARGGRVHQVATVNSDF
                     LARALVDPELLHILRHVDLATADGMPLVWASRVLGGSIPERVTGADLVPALAERFAAS
                     GRSLFLLGARPEVARAAAKRLCQRFPGLRISGVYSPPMADLESMDHATILRRVNAVRP
                     DALLVAFGSPKQEKWIYRHRHSLEVPVAIGIGASLDFIAGSARRAPVWMQHAGAEWLW
                     RLGNEPRRLWRRYAGDFRHLAPGLMRYWLLTRQTRSPRPPEEGADEIRAIGPRAIRVA
                     GRFGADQRGAFEALALQTLAGEASEPMQSAGATSPHGRPGHVYVDLTACTYIDSAGLG
                     CLASLAHEARARGYEFRVYPGSPAIRRLLGLGGLDQHLGTADDVEGVG"
     gene            complement(11720..12457)
                     /locus_tag="PLY56_00125"
     CDS             complement(11720..12457)
                     /locus_tag="PLY56_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HOJ19920.1"
                     /translation="MVERLGPEEGDERILRYYEARNVRLWGLRRRLVLRTIFFTWGLS
                     QASRSVLKRAFDLIVGLVAVIITLPIMLLTALAIKLDSPGPVLFVQTRVGRWGETFPL
                     LKFRSMYVDAEARRAELEALNEASGPVFKIRNDPRVTRVGRIIRKLSIDELPQLFNVL
                     AGHMSLVGPRPPLPSEVAQYTPEHRRRLDVKPGITCIWQVSGRSNVDFERWVMMDVEY
                     IEQESLWLDIRLLLRTIPAVLFGRGAY"
     gene            complement(12667..13521)
                     /locus_tag="PLY56_00130"
     CDS             complement(12667..13521)
                     /locus_tag="PLY56_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOJ19921.1"
                     /translation="MLRQLRIILDMIKFEHTVFALPFALMSAFLAAGGVPSGRALFWI
                     LVAMVGARSSAMSFNRLVDQEFDRRNPRTASRALPAGLLTRGQVWVFCLASAALFILA
                     AGMLNRLALILSPVALAIVWGYSYTKRFTSLSHLALGLGLGVAPVGAWIAIRGRIELV
                     PLLLCAAVTLWTAGFDIIYACQDTEFDRKEGLHSLPARLGERNALLLSILLHVGVAII
                     LFLLPTLVPLGPLYHAGAVAAVLLLAYEHTLVRPGDLSRVNAAFFTTNGFVSIGLMVF
                     TIADVLAR"
     gene            complement(13525..14124)
                     /locus_tag="PLY56_00135"
     CDS             complement(13525..14124)
                     /locus_tag="PLY56_00135"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOJ19922.1"
                     /translation="MSVSGSELLVAITGASGSVYGLRLIERAAPLVRRISVVVSRAGA
                     RVIAHEMGTPFDLEQPSLRAFLGRDPDNVRFHHPDDLFAPCASGSSAPDAMVVVPCTM
                     GTAARIAAGIAEDLIGRAADVVLKEHRQLILVPRETPLNLIHLRNLTTLAEAGALILP
                     ACPGFYHHPRSIEELVDFVVGRILDHLGLPNPQARWGEE"
     gene            complement(14216..14851)
                     /locus_tag="PLY56_00140"
     CDS             complement(14216..14851)
                     /locus_tag="PLY56_00140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ19923.1"
                     /translation="MRRSLPLLLAALVVVLTALPLLAAKAKAKPSGTLYANIVTNQGT
                     IVVQLFEKEAPKTVANFTGLAMGTKAFTDPATGKQVKRPFYNGLLFHRVIPGFMIQGG
                     CPYSRDPSRAGTGGPGYQFEDEFVSSLRHSKPGRLSMANAGPNTNGSQFFITVAPTPH
                     LDDRHTIFGQVVKGLDVAIKISQTPRSSNDRPLKPMVIKEIKVYRANSPKL"
     gene            15013..16074
                     /locus_tag="PLY56_00145"
     CDS             15013..16074
                     /locus_tag="PLY56_00145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ19924.1"
                     /translation="MMIRVGVAGLRRGLSLLNTFAMHAGVEVVAACDTDAALLERAGK
                     EMGIANLYQEYEDLVRHDLDCIVIGTPMPQHAAQAIAALEAGRHVFSEVPAVASLAEA
                     EALVRAARKARGKYMLAENCCYWAFIQSWRQSVSEGAIGKVIYAEAEYIHDCRGLMRD
                     ATGNPTWRTRLPPIQYCTHSLGPLLLITGDRCLTATGMHTGSNVAPDLGVIDMEVGLF
                     KGETGAVYKILCGFSLVREPSFHYYTLYGTHGCLERARGEDKTLAYFDSVPHLRGMIS
                     YPLSISHPRAPAGATVGGHGTCEYYMVTDFLEAIREDRNPPIDIDFAMDMTLPGICAH
                     LSAEQGGQPVPVPNSRDWV"
     gene            16174..16872
                     /locus_tag="PLY56_00150"
     CDS             16174..16872
                     /locus_tag="PLY56_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOJ19925.1"
                     /translation="MIRVRLAEERGQDVRDWLESRHTFSFGRYFDPQQMGFRQLRVIN
                     EDRIAGGGGFPTHEHADMEILTYVVEGALEHRDSLGTHSVIHADEVQRMTAGTGVQHS
                     EFNASATEPVHLLQIWITPERAGLAPGYEQRAFPKEEKRGRLRLVASRQGRDGSLSLH
                     QDVDLYACLLDAGGQVSHPLRSGRHAWAQVIEGKVSLNGQALGAGDGATVSDEAEVVL
                     SAAAPAHLLLFDLA"
BASE COUNT         2810 a         5655 c         5814 g         2820 t
ORIGIN      
        1 ggccgccgcc gatgccggcg cccgcgccgc ctccctggcc agggccaacc cctggccgcg
       61 gcgatacagg gacctcatcc gcttcgcgcg tagacggccc ggtcgccagg acacttgcgg
      121 tgcccccgcc gccaccgatg cgcggggtgg ccttgggcag gcgcatcccg cccgccccga
      181 agcggaaggt gaccgtttca ggcgctcgtg ctgcacggct cccaccgccg ggatcgtcgc
      241 ctgatccggg cgtgggcgca cccccgccaa cctggcgcga taccaatccg ggttcaggct
      301 ctcccgcggc atcgcgccgg ccgatgggga gaccgagtgg caggggctca cgcgcggcac
      361 ggcccgtgcc gccgggcagc gcctgcgtct caccgagcgg gtcgcctggg aaggagacct
      421 ccgggcgcgc gcgcgatagc cgacgcgggc cggccatgcc tcctccggga gcctcctcct
      481 ggcggttact tcccgggccc gccggcgtgc cggatccggg cgggttcggg cgcggtctcc
      541 actctgggaa ctccgcttcc gggagagcac gacgcgccat ctggacggcg cgggggcccg
      601 caggcacggg cgatggttct gcagcgcgcg agaccgtctc agaaaccgcg tcctgcttgc
      661 gcgcggctgg cgcggacggc ttgggacgtg gcgacgtgcg ggtggcaatc ggtgttttcg
      721 acacgcgccg cgcgggagac gctggacgtg gcagtgccga gcggctgggg cgcggcggtt
      781 tgtcggaagc cagagtcgtt ttggcaggcg ctggctttgg agccggcgcc ttctctggct
      841 ggatgacggc tacctcgatc acgcgtgcgc tcggtggctt acggggcggt ccttgcaggt
      901 gagcgaccgc cgcgaacccg aatgtgtgta gcaccagcga tccgagtagg aagggaccga
      961 tggagacgcc atctcggacc atggtcattc actccccgcg gggtgcccac gatgctggca
     1021 accggcagct tcacctccgc tcgtcagccg gaggcgcctc cacaatcggc tcggttgcca
     1081 gggcgatgtg ggtaaaaccg gccagtttca cctcgtcgag caccatcatg atcttcccgt
     1141 agggcacatg ctgatcgccc ttgatgatca cccgatcctc cgtgctcttg gaggcgcgca
     1201 tgcgcaggcg ggcgcgaagc tggttgggcg tggtctggcg cccatcgaag tagagcttct
     1261 gatcgcccgc aatcgctatg ctgatatcct gctgcttttc cggggcggct tccgtggtag
     1321 ctgccttggg tagcttcatg ttcagtcccg cctccttgac aaaggcggtg gtgaccatga
     1381 agatgatcag gagtacgagg gagatatcta cgagcgggat gatgttgatc tccgccatca
     1441 tcttcccatc gttgttaggc acccgcatcg atcgtctccc gctgcatgat gtaaggggtc
     1501 aggtcggagg catgcgagcc gactaagagg gtcaggtcgt tcaccttgcc gacgaagatg
     1561 ttgtaggcga tcaccgcagg gatggcaacg cccaatccga acgcggtcgc gatgagcgct
     1621 tcggcgatgc ctcctgccac gaccgagggg ccgccgaagc cttgctgggc gatcttgttg
     1681 aacgctcgca gaatgccaag caccgtaccg aacagcccga tgaagggcgc ggtgcttccg
     1741 acagtggcca ggatgcccag gtaacggctg aagcccgctg tttgcaaggc gagggcctgc
     1801 gcgatcccct ccttcacttg ttcggcgccg aactcgcttc gctccgcccc cgcgcgcaga
     1861 actggcgcca cgggcgcggg gtttgcctcc aggtcctgca acacctcatc gcgcgtgccg
     1921 ttgcgcacgc cctcacgaag ccggggatac tcctcctctt cgatgcggcg cagccggcgc
     1981 agcgtgtagg tgcgctccag gatcaccgcc accgaaagga tcgagtagac cacgagcggc
     2041 aacatgtaga cgaggaccag tggcccgccc tcgctgagga cacccaggtt gaacatcgtc
     2101 ttgaggcaac cttccttatg ggtcaagcgc cgccaggcgg gcacgtgctt gttctgccgc
     2161 ctggctctgc gggtattgct cgatcagggc gcggtaaacc gctatcgccc gcgtcttctc
     2221 cttggcgttc tcatacgcct gtccggcctg atactgtgcc ttcggggccc actcgctctg
     2281 cgggtagagc acggcgacct tcaggaactc ggtggccgcg tcggcgtgtt tcccctgcgc
     2341 ctggagtgct tcccccttgc gcagttgcag ctcggcggcg gcatcgcctt gcgccgcttt
     2401 caggccctca tcagcgaggt cagcggccgc ggccgcctcg ctggttgcca gcaggcagcg
     2461 caccaggccc agcctcacct gcgccgcaag cggtccgtcg ggatcggact gcagggcgcg
     2521 ctcgaagcaa ggacgcgcct cgcgccattg ctccgccttc gcgtgagaat cgcccgccca
     2581 gaaagaggcc tccgcccgct ccttcgcgcc cggggccagg ccggcggccc gggcgaacgc
     2641 ggcggccgct cccggatagt cgggcgtgta gtagagcgcc gcgccgaggc gcatggcgat
     2701 ctgatagccc aactcgccct ccggcttccg cgcctccgcg cgctggtagc gttgcacggc
     2761 ggcctgatac tccttccggg cgaagtactg ctcgcccgcc aggaagagcg cctctgcggc
     2821 atagcggcct tccgggtagt cggcagccag gcgctcgaac tccgcggcgg cgcgatcatg
     2881 ctctttcagc tccgcgtagt tccaggcgag atcataccgc gcccgctcgg cgaaaggccc
     2941 cttctcagcg gcgagctcca gagcggggat cgcctcccgg taggccttgt tttgcgtcag
     3001 cgcatagccc aggtagtagg cgatccgggg aaccatcggc gattccggca gcgcccgcgc
     3061 cttttccaac gccctctgcc agcgctgcgc ggccgttcgg taatccttct gggaatgcga
     3121 cgcccaggcg agggcgtaga gcgccgaaac cgtttccgtc ggccccgggt tagacgccag
     3181 agccttgttc gcggcctcct ccgcctcggc gtactggccg gccatcgtcg cggcctcggc
     3241 cagggtcagg aatgctggca cccgaatcgg cccttgggga ttgcgatccg taagacggcg
     3301 cgccgcccgc gccgcctccg ggtactgctt cagatcgagg tgggcccacg ccagctccag
     3361 gagtgcctgc tccaccagct tccccttggg atgctgctcg aggagctggg tgtacgcctt
     3421 gacggcgccg gccgcgtcgc gcgtctgaag gcatgccgct ccaagcagat aaagcgcctg
     3481 ctcggctgcc tcaggcgacg gctttccggc gagcacgcgc tcagcgagcg ccgccgcctc
     3541 tgcatagcgc tctgcggcaa actccttttg ggccagctgg atctgggcat cggccgcgat
     3601 gctgccgggc ggcagttttc cgacctcgcg caggagctgg ctcgtctcct cgccaccggg
     3661 cccggcctgc ttcagcaggt cagcgatggc ggcctgggct tcgcgcgcgg tgtcggtgtt
     3721 ggggtgcgcc tccaacacgg cgcggtaggc ggcagcggcg tcgcctgggc ggccggcatc
     3781 ggcgtggcaa tccccgatgc gcagccgggc gcgcgcggcg aaggcgccct tcgggaagcg
     3841 cgtcaggagc gccgtgtagc gcgcgatcgc ttcgtccggc ctgccggcgg cgcgcagcgt
     3901 gttcccggtc cagtagagcg cttcctcggc cagatcggcg tgcctactct cagccacgcg
     3961 ctcgtaggcc tccagcgccg cggggtactc cttcaggcca tagcgcgcgc cggcggccag
     4021 gatgtcggtc tccgcgcgct cgcggctcgc cgtctccgag agggtggcgc actccgcggt
     4081 ggcggccagc actgcgcgat aatcctttgc gtccagcgcg gcgtaggcgc ttccgagcat
     4141 cgcggccagg cggagggccg gggcgcgcgt gctcacgccg gcatacgcct ctcgcgcctc
     4201 gcggtggcgg cccagggcgc gcaaggcgtc gcccaggcgc acgcgcgact cgtcgatcgc
     4261 ttcgaattgc gggtagcgct ccaggatcac gcggagcgcg gcgatggccg cctggggatc
     4321 accacgttcc gccgcgcaag cgccgatcga gaggtaggcg tagggaacgt gcttgtgcgt
     4381 tgggtgcttc tgcaccaggg agcgataggc cgcctccgcc tgcgggtagc gcctgcgctg
     4441 gtactccgcc tcgcccagcc agtagcgggc ctgcgccagg gcttccgggt ccttgctcgt
     4501 ctcgatctgg cgggtgaaag tagctgccgc tttgtcaagc tgctccagcc ggaggtagca
     4561 ctgacccagg gagaagaggg cgccatccgc ctgcggatct ttcggatagg tgcgaagtgc
     4621 ggcctcgtag gcagcggctg ctttgaccca gtcgccactc ccccggtagc aatgtgccag
     4681 cagataggac gccgtggcgg cgcccgcgtg cttcggatgg ctcttgagga acttctcgaa
     4741 ctcgggtacg gcgagcgagt agagcttgcg ctcgtagaga cctttggcga aatcgagttg
     4801 ctgcttgggg tcgaccgccc cgagcgctgg caagagggag agcgccgcga gcacggcggc
     4861 cagagcccaa acacgccgtg ctgttccccc gcgggccgat ggggccacca ccgatgggcg
     4921 cgagggcagc aatggcgtgc ccggctgcct gttgaagacg cggaacaaaa gcatccaggc
     4981 gtgccgctgc ctccgagagg tgttgcccgg gagtgtggcg cactccccga agcgcctcgc
     5041 agagcgaccg cgcccctgcg gcttatccgc gacacgaagt atcatgcccg tccccactcc
     5101 cgttggcgag ttcgcctcac ttcccgccgg gaaccggatg caatgagtcc cgctggtcat
     5161 gcgagcccat ggttccaagg agaaccggcc gaacccaggg cgcgtcgctc ccaccgcagc
     5221 agaggcggcc aggctggccc tggtgggcga acacgcgcgt cgcttgttta gactcggcca
     5281 gccagggttt gtttcagcgg cgcgcctggg ggcgggatga gcttacttgg caggagactg
     5341 gccacagaga tgggcataga gccgggctac cttctccaca tagcgctgtg tttcgcggta
     5401 ggggggcacg cctccatact tgcgaacggc gcctgcgccg gcgttgtagc tcgccagggc
     5461 cagcttgagc tgctcccaga agggcttgtc ggcgtgctgt ttcaggtggc tgcgaaggag
     5521 cttgacagcg ccgtagatgt tctccaccgg atcgtaagcg tcgcgcacgc ccaggccggc
     5581 cgccgtgccg ggcatgagct ggcccaggcc catcgcgccc ttatgcgatg tcgcggtcgg
     5641 tttgaacccg gactcgacgg cgatcacggc gacgacgagg cgcgcgtcaa tcccgaagtg
     5701 cgcgctgtag gcgaggatac tccgcgcgat ggcctcttgt tgcgcgtccg agagccgcgg
     5761 gttgaagtag cggatggcac gcttgtaggc gtccaggtag ccggaccatc cccggctggt
     5821 catcgcctgc gtctgctgat tcgggcgcgt cttggaagcg ggaggccgcg aggccgtggc
     5881 gggctccttc cagcgtggtg gcggtggcgc tgagacaggg gatgccgccg gcgcaggaga
     5941 gggctgcggt ggctcggcag tctcacctgg cggttgggtg gcaggtgatg gctgcgaggg
     6001 gaatgtggtg ttctgggcgg gagagggaac ggtctgggtt gaatcggggc gtggctgggg
     6061 cttcggggcg tgcgccttct cccatgcggc agcgatactc tcatcgacca tggcgatcag
     6121 ctcgaggacg gaagcatcgg ctgctgccgc gcgcccgatc acgcgcaccc ggctgccaac
     6181 gataaacacc gggtagtcga cgggcgaggt gatctggtat ccgggtgcat cgtcggactc
     6241 ggcgcgaaga atccaggtaa ccgtctgcgc cgataccatt ctcccctgga tgagtccgcg
     6301 cacctcgacg acgttcgcgg cgttgccaag ctgctgcttt gaaacaagat aggcgcgcag
     6361 atactcctcg ggcgcggcgg ccgccgccgg gctagcgggc gcggcaagca ggaggcagcc
     6421 catcgccagc atcgcccaga gcactttctg ggtggcgcgc gtgtctcgat agagcctgtt
     6481 ccggcgtatg cgcatcgtta ctccctctgt cagacaccgc acgtgccatt tggcgtgcgc
     6541 ctccattata gcatacccgg cctcatgtgg ctataatccc tggcccggcc cccgctaact
     6601 gggaggtacc cccagggcgt agagacggcc tgcgatctcg tacgaggtca tcggcgtgct
     6661 caggagacag acgccctcct gagacgccac ttccagaaga tcatcctgcg gctcgcgatt
     6721 gccggcgagg atgaccgccg tgaagtcctt gagactggcg accgcggcga cgttccgatg
     6781 cgcctggatg gtaatccaga tgttcgcttc gcggccgttc gcgagcacat cactgagcag
     6841 gtccgagacg taaccgccgg tgacctcacg atcctggacc ggggtgaggg cacgcagtcc
     6901 gagttcatcg attacctggc tgaggcgcat ttactcccct ccctccgaca cgagcgggtg
     6961 caggtcgatc tccacttcca gacgcgtgcc ggaaccgggt ttcgattcga tgtggaaccg
     7021 gtccgcacac ttctggatgt tgggaagacc tatgcccgcg ccgaagccca tcacgcggac
     7081 caactcgctc gccgtcgaga aaccggggcg tagcgccatc tccacatcct caatcccggg
     7141 gccccaatcg accgcctgga tggtgatgct ctcggcgcca atccgggcgg tgatggcgcc
     7201 gccgatggag tggatgatga tattggtctc ggcttcatac gtggcgatgg ccgcgcggcg
     7261 agctaccttg atatcgatgc cgcgctcctt gagagccgcc ttgatccggg ccgaggcaat
     7321 ccccgcatta tcgaagtcgc ctgcgttcac ggggaactcc atggtcacct cctggccctg
     7381 ggtgatcgga gtgccggcgc gttggagcag cgcgatctcc cgccgggcgg cctcttcggc
     7441 gatgtggttc agctcctcca tcaggcgcac cacaatatcc gcctgggtca gaatcccgca
     7501 cagacatcct cccgcgtcga gcacggggat ccgaccgaaa cgatagcgcc ggaagtactc
     7561 caccgcccgg tgcaacggca tgttgtcctg gatggtgacg acgttggtcg tcatccaatc
     7621 tccgacgcgc tcctcgatat gcccgtgatc gagggcagag atgatatctt ccaggctaac
     7681 gatccccacg agacgcccat cttccagcac gggcgtgcca gagattcggc gctcgcgcaa
     7741 gatggcctgc acctcgcgca agctgttgtc gggggtgacg gtgaccacgt cgcgcgacat
     7801 ggcgtcacgc accttgagcg tgtaaatgag ctcatgggca acggaaaagc catttgtgtg
     7861 gttgatgacg ctcatggctt cgccagcgcc gcgaggcgca cgcacgactc gaagaggggc
     7921 agcgaggtcg tgtagagagg gatgccgtgg gcttgcgcca acccgatgat ctcctgagtg
     7981 gggtgcttgc cacgcaccag gaagactgcc agcgccccca ccacctgcgc ggtgcggatc
     8041 acttgggggc tcaccaggcc ggtgaggatg acgaactgct ccttgcccgt ggcgaggata
     8101 tcgctcagga ggtctgccgc tgcggcgcta tccacttcaa tgtcgggatc ttccgcgcgc
     8161 accacctctt ctgcctcaag tgcctgcgcg acttcgcgta gcaacatagt ctacctccga
     8221 acatggccgc actattatag cacaagatcg gcgcgggcgg aacgggcagg tgcgtgatgc
     8281 tacggaacag tgaaggtgga tgaaagggag accgtcagga tccgcgcgga tgcgcgagag
     8341 agcgcggcgc ggggacggtg ctcggcacgg caacaccggg cgttcccgtc ccgcactccg
     8401 gcttgcgacc caaacgcgag cagcagtggc gagggaatgg gtggagaaca cttcgaagcg
     8461 gggctgttgg cgtgctcaat ctttctggtt gccaacagcc cgcccacccg gccaggctgc
     8521 tccgggccga atggatcgct taggaaggaa gaacccgccg gggaataggg ggcgattctt
     8581 ccgtcgcgtg caagaaggag tgaaccgctt ccggtagggt gtcgtagatg tcgaatgcgc
     8641 ggtgcagtcg ggtgatctcg aagatgacac ggaccgggcc ttgaagcccg gcgaggcgca
     8701 ggtcgcccgc gctggagcgc gccatcttga gccccgatac gagggcggcc aggccaaccg
     8761 agtcgacaaa atcgacccgg ctgagatcgg cgaccaccca gcttttccct tcctctacga
     8821 gagtggccag tcgctgccgg aaggtgcccg cggtgcgcgc atccaggcgg cccagcggct
     8881 caatcacggc tactcctgtc tcgtcacgca actccacgat catccagtca ccccttcccg
     8941 agacggcaca cggcatcggt ggcgcgcgtt ggccccaggt atcatcatct cttgcgccgt
     9001 gttgccgccc agagtagtat tctcaggtca ttccataggc tcttcgatgc ggcgtagtag
     9061 gcatctaccg cgagcgtctc ctccaggggc aggtcatcgc ccccctggat tcgccatagg
     9121 ccggtgaagc ctgccggagc ctggcccacg tgccggagcc aatcctccga cacgctggct
     9181 gcctcctcct ccgtcatcgg tttgatgccg accagactga tgtctccgcg catcacgtta
     9241 tagagggctg gaagccgaga gactcccaac cgctccgcga gctttcccac tgccccgtga
     9301 gggctaaacc ggagaagctg gacggggcgc atgcgtgggg cgccggacgg caatctctgg
     9361 ccgggaaccg gcgcgaggcg ccatgttcgt tcgatggtgg gttggcgagt caaccgcgcg
     9421 acgacgccca gcccaaaaaa gatcggcagg ccaccgatga gcgcgagcgt gctgatcccg
     9481 aggtccgccg ctcgcgacca gatctgcccc cccggccgtc cccggatatg cgacaggagc
     9541 acgtcgtctt cgatgtaggc gctggtgccg caatcaaatc gcacgaccca ccgcccataa
     9601 gcgaggcacc tctccagctc caggagccga ccgatgccgt cgtcggcccc accacactgt
     9661 gcgccacgcg cgcctcctcg tcgacaacgc tgtgcggacc gaccacagtg ttggggccga
     9721 cgacggcacc gcgggcaatg cggcagtagt ctccgatcat caccgggggc tggatgttcg
     9781 cgtggggatg gatccgggtg tgatgcccca gccggatccc aggcgccacc tcgcgcgctg
     9841 gcaggtgcag gcgcgagtag gagccggaca ggatctcctg gtgaacctgg taatactcgc
     9901 tgaacgcccc gatatcgctc cacgggccct cgtagcagta tgcgtagaag ggaagcccgc
     9961 gttccagcag ctttgggaaa aggtcgcgcg cgaagtcaaa gaactcggct tgcgggatga
    10021 gatcgagcac ggagggctcc aggacgtaaa acccggtgct cacgagattc ccgcacgcct
    10081 cctcgggccg gggcttctct tgaaactgca agatacggcc gtcgtcatcc acccgagcca
    10141 ccccgaagcg gcccaccatc tcctggggca tcggcttgac gaccaccgtc accgcggcgt
    10201 tgcacgcctt gtgataggcc accacggcgg ccacgtcgat gtcccaaagg ctgtcgccca
    10261 caaggaccac gaaagtgtcg tcgaaaaacc gctgcacgcg cttcacggcg ccggcggtgc
    10321 cgaggagggt gctctcgagt gagtagctca gattggctcc ccatcgcctg ccgttgccga
    10381 agtgctgctc taccagatgg ggcaggtggt ggaggttcgc cttgatctcg gtgatgccgg
    10441 cgcgcaccag catctcgatc gtatgctcca tcaccggctt gtttgcgacg ggaagcatcg
    10501 gcttgggcgt gtcgcccgtg atcgggctca gacgcgagcc gacccccgcc gcgaggatca
    10561 ttgccttcat cctaccccct ccacgtcatc ggcggtgccc agatgctgat ccaggccccc
    10621 cagccccaac aaccggcgga ttgcaggaga cccagggtac acccgaaact cgtaaccgcg
    10681 tgcccgcgcc tcgtgcgcca gggaggccag gcaccccagg ccggcgctat caatgtaggt
    10741 gcaggctgtc agatccacgt aaacgtggcc cgggcggcca tgcggcgatg tcgcgcccgc
    10801 ggattgcatc ggttctgaag cctctcccgc gagtgtctgg agcgccagtg cctcgaatgc
    10861 ccctcgctga tccgcgccaa accgtccggc aacgcggatg gcgcgcgggc caatggctcg
    10921 aatctcatca gcgccctctt cgggcgggcg tggggagcgc gtctgccgcg tcagaagcca
    10981 gtagcgcatc agcccgggcg ccaggtggcg aaagtcgccg gcgtagcgcc gccatagccg
    11041 ccttggctcg ttgcccagac gccacagcca ctcggcgccg gcatgttgca tccagaccgg
    11101 cgcgcggcgc gcgctcccgg cgatgaagtc gaggctcgcg ccgatcccga tcgccacggg
    11161 tacctccagc gaatgccggt gccggtagat ccacttctcc tgcttggggc tcccgaaggc
    11221 gaccaggagc gcgtcggggc gtaccgcgtt gacgcgccgg aggatcgtgg cgtgatccat
    11281 ggactccagg tcggccatcg gtgggctgta gacgccgctg atgcgaagcc ccggaaaccg
    11341 ctggcagagg cgcttggcgg cggcccgcgc cacctcgggc cgggcgccca gcaggaagag
    11401 cgagcgcccg ctggccgcga agcgctccgc gagcgcgggc accaggtcgg cccccgtcac
    11461 gcgctcgggg atcgatccgc cgagcactcg ggatgcccac acgagcggca ttccatccgc
    11521 cgtcgccaga tcgacgtggc gcaagatgtg gagcagctcc ggatcgacca gggcgcgggc
    11581 cagaaagtcg ctattcaccg ttgcgacctg atgcacccgg ccgccgcgcg cgacgaactc
    11641 ttcgatccga tccagcgctt ctgcctgggt cacttcatcg acgggcaccc ccaggaccac
    11701 ggtcaggcgg cgcatcgcct cagtaagctc ctctcccaaa cagcacggct ggaattgtgc
    11761 gcaacaggag acggatatcg agccacagcg attcctgctc gatatactcc acgtccatca
    11821 tcacccagcg ctcgaaatcg acattgctgc gcccgctcac ctgccagatg cacgtgatgc
    11881 ccggcttcac atcgaggcgc cgtcgatgct ccggggtgta ctgcgccacc tcggagggga
    11941 gcggcggtcg tggcccgacc agactcatat ggcccgcgag gacgttgaac agctggggca
    12001 gctcgtcgat gctcagcttc cggatgatcc gtccgacgcg ggtgacgcga gggtcattcc
    12061 gaatcttgaa aaccgggccg cttgcctcgt tgagcgcctc cagttcggcg cgtcgtgcct
    12121 ccgcgtccac gtacatcgag cggaacttga ggagcgggaa ggtctccccc caacgcccca
    12181 cgcgcgtctg aacaaacaag accggccccg gggagtccag cttgatcgcc agcgcggtga
    12241 gcagcatgat ggggagagtg atgatcacgg ccaccaggcc cacgatcaga tcgaaagcgc
    12301 gcttcaggac actccggctg gcttgcgaga ggccccacgt gaagaagatg gtccgcagta
    12361 cgagccgccg gcgcaggccc cagaggcgca cattgcgcgc ctcatagtag cgcaagatac
    12421 gctcgtcgcc ctcctctggc cccagccgtt ccaccatcgc tgaccccctt ccacacctgg
    12481 cggcggcgtg cgcccacgga ccgcagtgag tgggtgcacg ctacgccgtc gcgctcagag
    12541 agtgcacgac cggtgccaga ggaaaaccgc gaatcgcggg ccgcgggggc gtgaatcagg
    12601 agccgggtgt ccgatctttc gggcggagag cgggggatgg gctggggcgc gggtgcccgc
    12661 cacccctcag cgtgcgagaa cgtcggcgat ggtgaacacc atcagaccga tgctgacaaa
    12721 gccgttcgtg gtgaagaagg cggcgttgac gcgcgacagg tcgccggggc ggacgagggt
    12781 gtgctcgtag gcgaggagga gcaccgccgc gacggcgccg gcgtggtaga gcgggcccag
    12841 agggacgagc gtggggagaa ggaacaggat gatggcaacc ccgacgtgga gcaggataga
    12901 gaggagcagc gcgttgcgct cgcccagccg ggccggcaga gagtgaagcc cctctttgcg
    12961 gtcaaactcc gtatcctggc aggcatagat gatgtcgaag ccggcggtcc agagggtgac
    13021 cgcggcgcac aggaggagcg gcaccagctc gatccgcccg cggatggcaa tccatgcgcc
    13081 caccggagcc acacccaggc ccagcccgag ggcgaggtgc gagagtgacg tgaaccgctt
    13141 ggtgtaggaa taaccccaga cgatcgccag cgccaccggc gagaggatga gcgccagccg
    13201 gttcagcatt ccggctgcaa ggatgaaaag cgccgcggag gccaggcaga acacccacac
    13261 ctggccccgg gtgagcaggc ccgccgggag ggcgcgtgac gcggtgcgcg ggttgcgccg
    13321 gtcgaactcc tggtcgacca ggcggttgaa gctcatggcg ctactgcgcg cgccgaccat
    13381 ggcgacgagg atccagaaga gcgcgcgtcc ggaaggcact ccgcctgcgg cgaggaaggc
    13441 gctcatcagg gcgaagggga gcgcgaagac ggtatgctca aacttgatca tgtctagaat
    13501 gatccggagc tggcgcagca tcgattactc ctctccccag cgcgcctgcg ggttgggcaa
    13561 gcccaggtgg tccagaatcc gcccgacgac gaaatcgacc aactcctcga tgctccgcgg
    13621 gtgatggtag aagccggggc acgctgggag aatcagagcg cccgcctcgg cgagcgtggt
    13681 gaggttgcgg aggtggatca gattgagcgg cgtctcgcgg ggcaccagga tcagctgtcg
    13741 gtgctccttc aggacgacat cggccgcgcg cccgatcagg tcctcggcta tgccggcggc
    13801 gatgcgcgcc gccgtgccca tggtgcaggg aacgacgacc atcgcatcgg gcgcgctgga
    13861 accgctggcg cacggggcga agaggtcatc cggatggtgg aagcggacgt tatccgggtc
    13921 gcggccgagg aaggcgcgga gcgacggctg ctcgagatcg aagggcgtgc ccatctcgtg
    13981 ggcaatcacg cgcgcgcccg cgcgggagac gacgacggag atgcgacgga ccagcggggc
    14041 ggcgcgctcg ataaggcgca ggccgtaaac gctgccgctt gccccggtga tcgccaccag
    14101 gagctccgaa cctgaaactg acacgcttcc tcccatgatg cgccgcaacg cccgccgcgc
    14161 gggcgggcga acccactacg tgaccgcccg cggggcgcct cgataccgga tggccctaca
    14221 gcttcgggct gttggcgcga tagaccttga tctccttgat caccatcggc ttcagcggcc
    14281 ggtcgttcga gctgcgcggc gtctggctga tcttgatggc aacatccaat cccttgacca
    14341 cctggccgaa gatggtgtgc cggtcgtcca ggtgcggggt gggcgccacg gtgatgaaga
    14401 actggctgcc gttggtattc ggcccggcgt tggccatcga gagccgcccc ggcttgctgt
    14461 ggcggaggct gctcacgaac tcatcctcaa actggtagcc ggggccgccc gtgccggcgc
    14521 ggctgggatc ccggctgtag gggcagccac cctggatcat gaagcccggg ataacacgat
    14581 ggaacaggag cccgttgtag aagggccgct tcacctgctt gccggtggcc gggtccgtga
    14641 aagccttcgt gcccatggcc agaccggtga agttggcgac ggtcttgggg gcttcctttt
    14701 caaagagctg gacaacaatg gtgccctggt tggtcacaat attcgcgtag agggttcccg
    14761 acggcttggc cttcgccttt gccgcgagga gggggagcgc cgtaagcacc acgacaaggg
    14821 ctgccagaag cagcggcagc gatctgcgca tgagtgactc tctcctttcg catgccgatt
    14881 gggtcgggtt gggccggacg ccaaggcgcc tggctgccgg cattatacca ggaagcctcc
    14941 tcgccgggca agtgagtgac cgtccggcag gaaccggaag gggcgcgcgg aacttcccca
    15001 aggaggaatg atatgatgat acgtgtgggt gtggccggcc tgcgccgggg cctcagtctg
    15061 ctcaatacct tcgcgatgca cgccggggtg gaggtagtcg ccgcgtgcga taccgacgcc
    15121 gcgctcctgg agcgcgccgg caaggagatg gggatcgcca atctctacca ggagtatgag
    15181 gacctggtga ggcacgacct cgattgcatc gtcatcggca cgccgatgcc gcagcacgcg
    15241 gcgcaggcga tcgcggccct ggaagcaggg cggcatgtct tcagcgaggt tccggcggtg
    15301 gcctcgctcg cggaggcgga ggcgctggtg cgcgccgccc gcaaggcgcg cggcaagtac
    15361 atgctcgccg agaattgctg ctactgggcg ttcatccagt cgtggcgtca gagcgtctct
    15421 gaaggcgcca tcggcaaggt gatctacgcc gaggcggagt acatccacga ctgccgcggc
    15481 ctgatgcgcg acgccacggg aaaccccacg tggcgcaccc gcctcccacc gatccagtac
    15541 tgcacgcaca gtctgggccc gcttctcttg atcacgggcg accgttgcct caccgctacc
    15601 gggatgcaca ccgggagcaa cgtcgctccc gatctgggcg tcatcgatat ggaagtgggc
    15661 ctcttcaagg gggagacggg cgcggtctac aagatcctgt gcggcttctc gttggtgcgc
    15721 gagccatcct tccactatta caccctctac ggcacgcatg gctgtctgga gcgcgctcgt
    15781 ggcgaggaca agacgctcgc ctacttcgac tcggtgcccc acctgcgcgg catgatctct
    15841 tacccgctct cgatcagcca cccgcgcgcc ccagcgggcg ctacggtcgg cggccacggc
    15901 acgtgcgagt actacatggt gaccgatttc ctggaggcga tccgcgagga tcgaaacccg
    15961 ccgatcgata tcgacttcgc catggatatg acgctgccgg gcatctgcgc gcatctctcg
    16021 gcggagcagg gtggccagcc tgtgcccgtg ccgaactcgc gcgattgggt ctaagctgct
    16081 acgacgtgtg ctctgaagcc ggaaacgggg tagaagcggt ttggggatgc ttcggccctg
    16141 gaacggatcg gatcgtcccc caaggaggtg gagatgatcc gggtgcgtct ggcagaggag
    16201 cgagggcagg acgtgcgcga ctggctggag agccggcaca cgttctcgtt cgggcgctat
    16261 ttcgacccgc aacagatggg attccggcag cttcgcgtga tcaacgagga tcgcatcgcg
    16321 ggcgggggcg ggtttcccac ccacgagcac gccgatatgg agatcctcac ctatgtcgtg
    16381 gaaggggcgc tggagcaccg cgactcactg ggcacgcatt ccgttatcca cgcggatgag
    16441 gtgcagcgca tgaccgccgg tacgggcgtc cagcacagcg agttcaacgc ctccgcgacc
    16501 gagccggtgc acctgctcca gatctggatc actccggagc gtgccggact tgcgcccggc
    16561 tatgagcagc gggcgttccc caaagaggag aagcgtggcc gtctgcgcct ggtggcttca
    16621 cgccagggtc gcgacggctc gctctccctc caccaggatg tcgatctcta cgcgtgcctg
    16681 ctcgatgcgg gcggccaggt gtcccacccc ctccggagcg gccggcacgc ctgggcacag
    16741 gtgatcgagg ggaaggtctc gctgaacggg caggcgttgg gcgccggaga tggagctacg
    16801 gtgagtgatg aggcggaggt ggtcctctct gccgccgcgc cggcgcacct gctcctcttc
    16861 gatctggcct gagccagcgc ccgttgcccc catgcggcgc ttttcgctca ccgcccgata
    16921 ctgcccggcg tgctgccggc cccgctatcg gccctccccg cgcgcgggga tggcgcatcg
    16981 acgaaatctc cccaccagaa agcaggatgg ctctgccctg gcactcttct tgcacccgcg
    17041 ggctccggat gcgataggct cggccctatc gtgggaggag cttccatggc agagattgc
//
LOCUS       DAOSCV010000003        14496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_2824_length_14496_cov_3.232117, whole genome shotgun sequence.
ACCESSION   DAOSCV010000003 DAOSCV010000000
VERSION     DAOSCV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 14496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14496
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_14496_cov_3.232117"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLY56_00155"
     CDS             complement(<1..1520)
                     /locus_tag="PLY56_00155"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ19926.1"
                     /translation="MADDLSIARAAVLRPVTEVAACLGLRPDELELYGEHKAKIKPEA
                     LARRADAPMGKLLLVTAMTPTPAGEGKTTLAIGLAQGLARIGQRACVALREPSLGPVF
                     GVKGGATGGGYAQVLPMEEINLHFTGDIHAVTTAHNLLAALADNALHQGTGPDLDPRR
                     IAFPRVLDLCDRALRHVVLGLGGPADGVPRQGGFEITAASEVMAILALASGLQDLRER
                     LGRIVIGYTTAGTPVTAAEIEATGAMLAVLRDALKPNLVQTIEGGPAFIHAGPFGNVG
                     PGVSSLMATRLGLQTADYCVTEAGFGSELGAEKFFDIKCRCGGLTPNAALVSATIRAL
                     KYQAGKPLAALAEPDVDAVGAGLANLEKHVENVQAFGVPVGVALNRFTTDTREEIDAV
                     FEAMARRDVPVALADVWARGGEGGRDIAKMMVDLAEQPSGYRPLYDLEEPVRAKIEKI
                     VTRMYGADGVEYSREAEKRLLEIEAHGYGNLAICMAKTQNSLSDQAALRGRPTGFKV"
     gene            complement(1546..2340)
                     /locus_tag="PLY56_00160"
     CDS             complement(1546..2340)
                     /locus_tag="PLY56_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HOJ19927.1"
                     /translation="MLIIGERLNSTRKSVGEAVNARDTQFVREEAVRQAEAGAHFLDC
                     NAAVVGVEAEPEALVWLVETCQEATGLPCAIDSPNSAALEAALKVHKGQAVINSITAE
                     REKFARILPLVTEHQAKVVALCMGDEGIPQTAQARIQAAEFLMENLTAAGVMPDQIYL
                     DPLVYPVSTNSHAGLDVLETIRHIHATHPGVHTICGLSNVSYGLPARKNLNRAFLVLC
                     MGAGLDAVIADPLDEALMALVYASEALLDRDEYCANYLSLARQGKL"
     gene            complement(2709..3353)
                     /locus_tag="PLY56_00165"
     CDS             complement(2709..3353)
                     /locus_tag="PLY56_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ19928.1"
                     /translation="MPSLLENLAQYVIDGNAQKAKETTDEALAQNVPVADIINKGLVA
                     GMQVVGQKFKCNEFYIPEVLIAARAMKQSMEKLRPLIAESGIQPIARVAIGTVRGDLH
                     DIGKNLVGMMLEGAGFEVIDLGTDVSPEKFVEAVREHKAEAIGLSALLTTTMPAMKET
                     IAALEEAGLRDKVAVLVGGAPLTQAYADEIGADGYAPDAASAVDTAKQLLRITA"
     gene            complement(3744..4397)
                     /locus_tag="PLY56_00170"
     CDS             complement(3744..4397)
                     /locus_tag="PLY56_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ19929.1"
                     /translation="MASESVQDYLKTIYKLSRDQESVSTSAIAARLDVAPASVTKMIK
                     RLADEGYVRHIPYQGVVLTKAGEQVALRTIRHHRLLELYLTQALGYPWDLVDAEAERL
                     EHVISEEMESRIDAVLGYPKVDPHGHAIPCREGKLAVEKCSSLAETEPGMRVRVRQVD
                     DASPEVLRHLGQLGLYPGAVVTVHERLPFGGHLRIEVQGREAHLGVEPAGHVFVAPE"
     gene            5033..5659
                     /locus_tag="PLY56_00175"
     CDS             5033..5659
                     /locus_tag="PLY56_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ19930.1"
                     /translation="MTGVSTIEALPAADERSQYAGDVALVQRAQAGDLEAFETLFDRY
                     HGRIYNIVYGMVSNPEDAADLAQEAFVKAYRAIGGLRDGQAFFAWICRIAVNLCRNFR
                     RGRPSTPHLSLEEGYCRDGESTPLDVADSSYEPARLVEIQATAEVVRRAIDTLSPDHR
                     EVVVMHHLQGLPVDHIAQVVGVPVGTVKSRLSRGRDSLRRALHGVLDG"
     gene            5777..5874
                     /locus_tag="PLY56_00180"
     tRNA            5777..5874
                     /locus_tag="PLY56_00180"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5811..5813,aa:Sec,seq:tca)
     gene            6122..6967
                     /locus_tag="PLY56_00185"
     CDS             6122..6967
                     /locus_tag="PLY56_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ19931.1"
                     /translation="MPREVQQAQQRRSRRIATALIAVAVVIAGLLVARSISGPQARFR
                     NVPPSEQHNHAGETTVSGIPSAVTMPPAAPARPDAGEAPPALAEPSSLARSFPTQTPG
                     EPPVLAQQPALQNPVLAELQGSILELESAPVSERDTYRWNVAAGDCYWLYARELALSS
                     PSAPREHEGERALRRLREDRFATSFPSSQEHLRRAELAYRRALELASTPEHRARVHQR
                     LGDILCARGKYAQAWEHAQRAQSLIGDDPRTAFLRARCLLGMKQPREKRASSRKQSTG
                     GHDNG"
     gene            6954..8678
                     /gene="murJ"
                     /locus_tag="PLY56_00190"
     CDS             6954..8678
                     /gene="murJ"
                     /locus_tag="PLY56_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ19932.1"
                     /translation="MTMGDSRGETLRDRLTKATLLITLVGLISRVLGYLRDAVIAYKF
                     GLEGEPSAYYAAIHVPDILNYLVAGGTLSSTFVPLFKEYWQRQRQSAAWAFFAVVASL
                     ILVLIVTLVILFEIFAEPLVHFMYGPHKGIKGLSPEQLELAVRITRIVLPAQIFFVIG
                     GLINGVIFSIRGDDKWYVAMPTFGALIYNVGIIAGGLMLANSHGIVGFAWGGLVGAFV
                     GPFLLVALAAQRAGAQFRFSLNLRHPGIRRYILLTLPLMVAVSYSFVDQIIASILGAR
                     LPDGSIAALNNAYRLMLVPVGIIGMAAATAAVGTLAGLAAENDVQGFRQQIREAMGRV
                     GVMVFPIIAVMIVLAPALVRILYQHGQFDADDTARVASLLRWYALGIYAWSVNYVLAR
                     GMYSLQDTITPAVLGTLTTLLIIPLSILLMRPMGAEGLALATTLGISLQSLAIFLALR
                     KKLRGLHAPAIGRSLKRMMVSAGLTALVCWGVQQLLHAIPTLPATGDTAQAALARLSL
                     LAPASGSIAGALLEVGIVAGIGIPLFAVIAVRLGEEEAAYLWGKIFSKATRLLRRSGS
                     SRHPAGRQ"
     gene            8812..9801
                     /locus_tag="PLY56_00195"
     CDS             8812..9801
                     /locus_tag="PLY56_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ19933.1"
                     /translation="MAVLKLALIGAGGMGMSLAKAAKTREDVLFVGVADPVAEQASKA
                     TAELGGEAYSSYADLLARNDLDAVLVATPNAAHCEVVLAATAAGKHAFCEKPMALNIA
                     DCDRMIAAAQNAGVKLMVGQVLRLIPGFAKARELVESGEFGKPLAIAIERSSFWKPRA
                     WRREYRPTGGVLFEINAHEIDYMRAILGDAASVYACISTETCPDSDIPGINYVLVRFK
                     EGGIGMLNSNSLIPQGQYQVAITLEKGSIRCDWSSVTYQFRDREAQAVPEEEMKAMLP
                     GVQREIDSFVEWVLYGKEPLVTALDGRSAVEIAQAALQSGATGQPVSLPLQSS"
     gene            10003..10078
                     /locus_tag="PLY56_00200"
     tRNA            10003..10078
                     /locus_tag="PLY56_00200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10036..10038,aa:Arg,seq:tct)
     gene            10171..10902
                     /locus_tag="PLY56_00205"
     CDS             10171..10902
                     /locus_tag="PLY56_00205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ19934.1"
                     /translation="MALILIAGILVVAAGCRRQTPPKKGKTPPAPKTVDAPRAPLPPT
                     PPGMAVSGFWATWGRPLHRCLEEHRARSGRQCHGALSIVIEKSKRRMVVRCDGEPVKG
                     YFIGLGRNPKGAKERRGDGRTPAGHYYLWRKNRQSRFYLSLGVSYPNRQDADRGLALG
                     IIDQKTHARICRALQRGSPPPQDTRLGGDIYIHGGGVGKIVRMDGKRFVHVRDWTEGC
                     VALRNSDMEELMELLPVGTPIEIRE"
     gene            11165..11476
                     /locus_tag="PLY56_00210"
     CDS             11165..11476
                     /locus_tag="PLY56_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19935.1"
                     /translation="MPALSQVVEKSLCRACSGRLVVSVAEGERLQECPQDEERQAECL
                     ARLMYRLACLDEALRYYASGKVAGTPELRDIIDFWAKEAMGPEADPNRLCFRDILLRQ
                     R"
     gene            complement(11563..13185)
                     /locus_tag="PLY56_00215"
     CDS             complement(11563..13185)
                     /locus_tag="PLY56_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOJ19936.1"
                     /translation="MPASLDLRFDRFYSYQELTRALEQLAAFAPDRVRRHSIGRSPEG
                     RELWMLTVTDFTRGDPDRKPAYLIHGNLHAQELSGTTAALYVAHYLVTDDTPELQRLL
                     GEVVFYIVPRINPDGAEYALTTGGAIRSRLVPVPARNAIRPADVNGDGLILRMRIRCE
                     DGDQRPDRQDPRLMVPREPHDDEGPFYRVFTEGFIEDWDGGEPREASYHKDFNRNWGA
                     NWRPEHQQYGAGDFPFSEPEMRAVAEFVAAHPNIFGALGFHTGCNGVLRPPASRPDEE
                     LNDRDLAVLRELGRKGEQATGFKLRAVTQYRLDESRPNRLQGHFTDWAYEHLGIYPFE
                     IELGMLYQSAGITTDQYFASKSDEERREFDRAILRWHDAHPEAGAFVDWQPFEHPQLG
                     RVEIGGLRMNAFANPDPRELPGIAEGCTRFILEHAGRYPHLRIVGVRVDAVGSDIFRI
                     RATVMNTGGFPTYIAETGRDVRANRSPMARLELGDGMEMLSREAGVELGRLGARSGKR
                     DLEWFVKGRGTARLVASAVKAGTAAVEIPIGG"
     gene            13431..13522
                     /locus_tag="PLY56_00220"
     tRNA            13431..13522
                     /locus_tag="PLY56_00220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13467..13469,aa:Ser,seq:gga)
     gene            13572..13839
                     /gene="ffs"
                     /locus_tag="PLY56_00225"
     ncRNA           13572..13839
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLY56_00225"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            14074..>14496
                     /gene="dnaX"
                     /locus_tag="PLY56_00230"
     CDS             14074..>14496
                     /gene="dnaX"
                     /locus_tag="PLY56_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ19937.1"
                     /translation="MSTYISLYRKYRPQTFADVVGQAHITRTLQNALEGGRVAHAYLF
                     CGPRGTGKTTTARLLAKALNCSRGPTRDPCNECESCRRIAAGTALDVIEIDAASNRGI
                     DDIRELRDKVKYAPAEARHKVYVVDECHMLTTEAFNALL"
BASE COUNT         2435 a         4859 c         4670 g         2532 t
ORIGIN      
        1 accttgaacc cggtcgggcg cccgcgcagc gccgcctgat ccgagaggga gttctgcgtc
       61 tttgccatgc agatggcgag gttgccgtag ccgtgcgcct caatctccag caggcgcttc
      121 tccgcttcac gcgagtactc cacgccgtcg gcaccataca tgcgcgtcac gatcttctcg
      181 atcttggcgc gcacgggctc ctccaggtcg tagagaggac ggtagccgga cggctgctcg
      241 gcgagatcca ccatcatctt ggcaatgtcg cgtcctccct cgccgcccct ggcccacaca
      301 tcggcgaggg cgacgggcac gtctcggcgc gccatcgcct cgaacaccgc gtcgatctcc
      361 tcgcgcgtat cagtggtgaa gcgattgagg gccacgccca cgggaacgcc gaaggcctgc
      421 acgttctcta cgtgtttttc caggttggcc aagcccgccc ccaccgcgtc cacatccggc
      481 tccgccagcg cggcaagcgg cttccccgcc tggtacttta gggcacggat ggtggcgctg
      541 accagcgccg cgttcggcgt caacccaccg cagcggcact tgatatcgaa gaacttctcc
      601 gcgcccaact cggatccaaa gcccgcctcg gtgacacagt aatccgcggt ctgcaagccg
      661 aggcgcgtcg ccatcaggct gctcaccccg gggcccacgt tgccgaaggg gccggcatgg
      721 atgaacgccg gcccgccctc aatcgtctgc accaggttcg gcttgagcgc atcgcggagg
      781 acggcgagca tggcacccgt cgcctcgatc tcagcggcgg tgaccggcgt gccggcggtg
      841 gtgtagccga taacgatccg ccccaggcgc tcgcgcagat cctgcaagcc gctggccagg
      901 gccaggatcg ccatcacctc cgaggccgcc gtgatctcga agcccccctg gcgaggcacg
      961 ccgtcggcag gaccgcccag tccaagcacg acatgacgca gggcgcgatc acacaggtcg
     1021 agcacacgcg ggaacgcgat gcgtcggggg tccagatcgg gccccgtgcc ctggtgcaag
     1081 gcgttatcag ccagggcggc gagaaggttg tgcgccgtcg tcacggcatg gatatcgccg
     1141 gtgaaatgga ggttgatctc ctccatcggg agcacctggg catagccccc tcccgtggcg
     1201 ccgcccttca cgccaaaaac cggcccgagc gagggctcgc gcagggcgac acacgcgcgc
     1261 tgcccaatgc gcgccaggcc ttgggccagg ccaatcgcga gagtggtctt tccctccccg
     1321 gcgggcgtcg gcgtcatggc agtgacgaga agcagcttgc ccattggcgc gtcggcgcgg
     1381 cgcgccagcg cctccggttt gatcttcgcc ttgtgctcgc cgtagagctc cagctcgtcc
     1441 gggcgcaagc ccaggcatgc agccacctcg gtcaccggcc gcagcacggc cgcccgggcg
     1501 atggaaagat catcggccat cgtgttcctc cgatgtcgcg tgtgactaaa gctttccctg
     1561 gcgcgccagc gaaaggtagt tggcgcaata ctcatcccga tccaggagcg cttcgctcgc
     1621 gtagacaagc gccatcagcg cctcatcaag cgggtcggca atcaccgcat ccagccccgc
     1681 gcccatgcag agcaccagaa acgcgcggtt gaggttcttg cgcgccggca gcccatagga
     1741 gacgttgctg agaccgcaga tcgtgtggac gcctgggtgc gtcgcgtgga tatggcggat
     1801 cgtctccaag acatccagtc ctgcgtggct gttggtgctc accgggtaga cgagcgggtc
     1861 gagatagatc tggtcaggca tcactcctgc cgccgtgagg ttctccatca ggaactcggc
     1921 cgcctgaatc cgcgcctggg ccgtctgggg gatgccctca tcccccatgc agagcgccac
     1981 caccttcgcc tggtgctccg tcacgagcgg cagaatgcgc gcgaatttct cgcgctcggc
     2041 agtgatggag ttgatgaccg cctggccctt gtgaactttc agcgcggcct ccagcgccgc
     2101 cgagttcggc gagtcgatgg cacagggcaa cccggtagcc tcctggcacg tctccaccag
     2161 ccatacgaga gcctccggct cggcttcgac gcccacgacc gcagcgttgc agtcgaggaa
     2221 atgcgcgccg gcttccgcct gccgcaccgc ttcctcgcgg acgaactgcg tatcgcgtgc
     2281 gttcaccgcc tcgccgacac tcttgcgggt gctattcaac cgctcaccga tgatcagcat
     2341 tgacttacct cccggggcgc accgccgtgg cacatcggga tgaggaggcg cgaccagcgc
     2401 gcgcgaatcc tctgcggggt agatgagggg atagggagag cggtgcccta tcgcggcctc
     2461 tctggagccg gcgccgtcag catgcgcgac cgccggccct ttgaaagcag aggcggatca
     2521 ggattcgctc tcccaaaaag aaagggcgag gcccgggcga acacacgccc tcagatctcg
     2581 cgggcgcccc atcatgaact gctaaaggcc acgccctcgt ctcccgcgtg gaggcggcgg
     2641 cgcgttcagg gacgccgaga tagggaacgt tcttgctcgt ccgaaacgcc tcgccccaaa
     2701 aagcgtgctc aggcggtgat gcggagcagc tgcttggccg tatcgacggc gctcgcggca
     2761 tccggcgcat agccatcggc accgatctcg tcggcatacg cctgagtgag cggcgcacca
     2821 cccaccaaca cggcgacctt atcgcgcagg ccggcttcct caagcgcggc gatcgtctcc
     2881 ttcatcgccg gcatcgtggt ggtcagcaac gccgaaagcc caatcgcttc cgccttgtgc
     2941 tctcgcaccg cctcgacgaa cttctccggg ctgacatccg tgcccaggtc gatcacctcg
     3001 aagcccgcgc cctcgagcat catgcccacc agattcttgc cgatatcatg gagatcgccg
     3061 cggaccgtgc cgatggcaac ccgggcgatg ggctggatac cggactcggc gatgagcggg
     3121 cgcagcttct ccatcgactg cttcatcgcg cgtgcagcaa tgaggacttc ggggatgtag
     3181 aactcgttac acttgaactt ctggcctacc acctgcatac cggcgaccag acccttgttg
     3241 atgatgtcgg ccacgggcac attctgcgcc agcgcctcgt cagtcgtctc cttggccttc
     3301 tgcgcgttgc cgtcgatgac atactgcgcc aggttctcca gcagggacgg catagtgtct
     3361 cctcctcgtt tgcttcgtta ctggccgggc ccgcataccc agcgcagtgc ttcccacgcc
     3421 gccgtttcca gcggttccca ccgataacac agcgcatccg cgagggacac gccccgttcg
     3481 cggggaatcc tgcgcgagtt tggccccccg agcgaactcc tggccggccg cagccggact
     3541 tcccaccgca ttttattccc ggcgtccggg cgcctttcct gctggcggcc gttggtattc
     3601 tcgtgcgatt cgctggtatt ctggtgtgca atcggccatc cacctcgccg ggtccggacg
     3661 cccgtggcag gcagaagcgg cgggtgtccc cacgcgtctc aacgggggag agcgacgctc
     3721 gcgcgagaca gcccggggag aagttactcc ggggccacga agacgtgtcc ggcgggttct
     3781 actcccaggt gcgcctcgcg cccctgcacc tcgatgcgca ggtgcccacc gaagggaagg
     3841 cgctcatgta ccgtcaccac agcgcccggg tagagaccca actgtccgag gtggcgcagc
     3901 acctccgggc tggcatcatc gacctgacga acgcgcacgc gcattcccgg ctcggtctcg
     3961 gcaagcgagg agcacttctc cactgccagc tttccctccc ggcacggaat ggcatggcca
     4021 tgtggatcca ccttcgggta gcccaacacc gcgtcgatgc gcgattccat ctcctcggag
     4081 atcacatgct cgaggcgctc ggcctccgca tcgaccaaat cccacgggta tccaagcgcc
     4141 tgggtcaaat agagctccag gaggcgatgg tgccgaatgg tgcgcagcgc cacctgctcg
     4201 cccgccttgg tgagcacgac accctgatag gggatgtgcc gcacgtaacc ctcgtccgcg
     4261 agacgcttta tcatctttgt taccgatgcc ggcgccacgt cgaggcgcgc ggcgatcgcc
     4321 gaagtagata cgctctcttg gtcacggctc agcttgtaaa tggtctttag gtaatcctgt
     4381 acggactcac ttgccatagt gctctcgcgg actctccgat ctacaggcca gccccgctag
     4441 taggcgcggg gctgctctca acgttcccat catacgaccg aagttggcat catgtcaaga
     4501 atctggtgac gctacgcggt cgcaccacac ccatcgcgcg ttcttcgacc ccttattctc
     4561 cgatgagggc cgcgcggttg ctccccctcc ccggtgtcgg cccgggatcg gaaggtaggg
     4621 agaccacaag gcctgcactt cctcatcagt ccatcaaccc cagcattgag cccctggcgc
     4681 gccctttttc catagggcct gaagcgttgc ggcggctatc agagcttgct cgcccgccga
     4741 gggcgcgata cgggcatacc ctgagcgtaa tacaggtttt cccgtatagc gatctgcggc
     4801 cgggcgtgat agaatggcgg taacacgcag gcttggcgga acctgacaga agttcggagt
     4861 gtcgccagat ctgacgtggc agatggttcc tgagaagatg aggccgggac gtgctccagg
     4921 gagcggcagg cctccgcatc tgacaaggcg caggtgacgg gaatcaggac ccccggtgtt
     4981 ggcctcacgt tccgtgcgca ccgcgggcag ccagatctga gaaggagggg gcatgacagg
     5041 ggtttcgacg atcgaggcat tgccggcagc agacgaacga tcccagtacg cgggagacgt
     5101 tgcgttggtg cagcgcgcac aggcaggaga tttggaggcg ttcgagacgc ttttcgaccg
     5161 ctaccatggc cgaatctaca acatcgtcta tggcatggtc tccaatcccg aggatgccgc
     5221 cgacctggcg caggaagcct tcgtcaaggc ctaccgggcg atcgggggcc ttcgtgacgg
     5281 ccaggcgttc ttcgcatgga tctgccggat cgcagtcaac ctgtgccgca atttccggcg
     5341 tggccgccca agcacgccgc atctctccct ggaagaaggc tactgccgcg atggcgagtc
     5401 gacccctttg gatgtggctg atagctccta cgaaccggcc cggctcgtcg agatccaggc
     5461 gaccgccgag gtggtccgca gagcgattga cacgctctca cccgaccatc gggaggtggt
     5521 cgtgatgcat caccttcagg gcctgcccgt ggatcacatc gcgcaggtgg tgggcgtgcc
     5581 ggtaggcacg gtgaaatcac gcctctcccg ggggcgtgat agccttcgcc gcgcgcttca
     5641 cggcgtcctg gacggctgag ccttcgtctg gcactccgcc ttagctcagg tctcttgacc
     5701 cgcgccgcgt ctcgtggtag gatggtcatg gaagcgcacg cgtgtctggc tgggcgcgcg
     5761 gcgccacttc caggatgagg gcgggtctct tctggtgaag ggcctgggct tcaaaccctg
     5821 cgcggggcag cgagagctgt cctgggtgag ttcgattctc acacgccctc gccagttcct
     5881 ccgataggga tgctgggtcg ggtgacgcgc ctaaaacacc gccatcaccc gccacgcact
     5941 ctcccatcgc cgctgctgcc ctcccccgga cacgccgcga tggggttgct tgggaaggcg
     6001 cgctatgaat gccgattggc cggccatggc cgaaggcccg cgttgcgaac gctgcggaca
     6061 ggtgcatctt tctctgccac agacaggccg cttcggctgc gggcgctgcg gttggccgtt
     6121 ggtgccccgc gaggtgcagc aggcacagca gagacgctct cgccggatcg cgaccgcact
     6181 catcgcggta gcggtcgtta tcgcaggcct gcttgtcgcg cgctccatct cgggcccaca
     6241 ggcgcggttc cgcaatgtgc ccccctccga gcaacacaac cacgcgggag aaacaaccgt
     6301 ctccggcatt ccatcggctg tcacgatgcc gccagccgcg ccggcccggc cggatgcggg
     6361 cgaggcgccc ccggcgctcg cggagccttc atcgctcgcg cgctcgttcc caacccagac
     6421 gccgggagag ccgccggtcc tcgcgcaaca accggccctg caaaacccgg tgctggcaga
     6481 gctgcaaggc tcgatccttg aattggagag cgctcctgtg agtgagcgcg atacctaccg
     6541 gtggaatgtg gccgcgggag attgctattg gctctatgct cgcgaattgg cgctctcctc
     6601 cccttcggcc ccccgcgagc acgagggaga gcgcgccctg cgccggctgc gcgaggaccg
     6661 cttcgcaacc agctttccgt cctcgcaaga gcacctgcgg cgcgcggagc tcgcttatag
     6721 gcgcgccctg gaactggcct cgacaccgga acaccgcgcc cgcgtgcacc agcgtctggg
     6781 tgacatcctc tgcgcgcggg gcaagtatgc acaggcatgg gaacacgcgc agagagcgca
     6841 gagcctgatt ggtgatgatc caaggaccgc cttcctgcgc gcccgctgcc tgctggggat
     6901 gaagcaaccc cgcgagaaac gcgcctcttc aagaaagcag agcactggtg ggcatgacaa
     6961 tgggtgattc caggggcgag acgcttcgtg accgcctcac caaggcaacg ctgctgatca
     7021 ccctcgtcgg cctgatcagc cgcgtgttgg gctacctgcg tgacgcggtc atcgcctaca
     7081 agttcggcct ggagggcgag cctagcgcct actatgcggc catccacgtg ccggatattc
     7141 tcaactacct cgtggccgga ggcaccctca gcagtacctt cgtcccgctt ttcaaggagt
     7201 actggcagcg ccagcgccaa tcggcggcct gggccttttt cgccgtcgtt gccagcctga
     7261 tcctggtact catcgtcacc ctggtaatcc tcttcgagat cttcgcggag ccgctcgttc
     7321 atttcatgta cggcccgcac aaggggatca agggcctcag cccggagcag ctggaactgg
     7381 cggtgcggat cacccgcatc gtgctgcccg cgcagatctt ctttgtcatc ggcgggctga
     7441 tcaacggcgt gatcttcagc atccgcggcg acgacaaatg gtacgtcgcg atgcccacgt
     7501 tcggggcgct gatctacaac gtcggcatca tcgcgggcgg gctgatgctg gcgaactcgc
     7561 acgggatcgt cggcttcgcc tggggcggcc tggtcggcgc gtttgtcgga cccttcctgc
     7621 tggtggccct ggcggcccag cgcgcgggcg cgcagttccg gttcagcctg aacctgcgcc
     7681 accccgggat tcggcgctac atcctcctca cgctgccgct gatggtggcc gtctcctatt
     7741 ccttcgtcga tcagatcatc gcgagcatcc ttggggcacg cctgccagac ggatcgatcg
     7801 cggcgctgaa caacgcctac cggctgatgc tcgttccagt gggcatcatc ggcatggccg
     7861 ccgccaccgc ggcagtcggc acgctcgccg gtctcgcagc cgagaacgac gtgcaaggtt
     7921 tccgccagca gatccgcgag gcgatgggcc gcgtcggggt gatggtcttc ccgatcatcg
     7981 ccgtcatgat cgtgctagca ccggcactgg tgcgcatcct ctaccagcat ggccagttcg
     8041 acgcggacga taccgcgcga gtggcctctt tgctgcgctg gtacgctctc ggcatctacg
     8101 cctggtcggt gaactatgtt ctcgcccggg ggatgtactc gttgcaggat acgatcacgc
     8161 cggcggtgct gggtaccctg acgacgttgc tgatcatccc gctaagtatc ctgctgatgc
     8221 gcccgatggg cgcggagggg ctggcgctgg caacgaccct ggggatctcg ctgcagagtc
     8281 tggcgatctt tctcgccctg cgcaagaagc ttcggggcct gcacgcgccc gcgatcgggc
     8341 gctcgctgaa gcggatgatg gtatcggctg gactcacagc cctggtgtgc tggggcgtcc
     8401 agcagctgct gcatgctatt ccaactctgc cggctacggg cgacacggcg caagccgcgc
     8461 tggcccgcct ctccctcctt gctccggcat ccggaagcat cgcgggcgcc ctgctcgagg
     8521 tcggcattgt ggcggggatc gggataccgc tcttcgcggt aatcgccgtg cggttgggcg
     8581 aagaggaagc cgcctaccta tggggaaaga ttttcagtaa ggcgacgcgg ctcctgagac
     8641 gttcaggttc ttcgaggcac ccggcaggcc gacagtgaag gccgtcctct cgcggaatga
     8701 cagaagggcg ggacgacggc tctccggatc cgggcataca gcagcggccg gcgctgcccc
     8761 acactcgcgt gcccattcga tccaagcagc gcatggctgg aggagcatca aatggcagta
     8821 ctgaaactgg ctctcatcgg ggccggcggc atgggcatgt cgctggcaaa ggcggcaaag
     8881 acgcgtgaag atgtgctctt cgtgggtgtc gcggatccgg tggccgagca ggcctcgaag
     8941 gcgacggcgg agctgggcgg ggaggcgtac tcctcctatg ccgatctgct cgcgcgcaac
     9001 gatctcgatg ctgttctggt ggcaacgccg aacgccgccc actgcgaggt ggtgcttgcc
     9061 gccaccgccg ctggcaagca cgccttctgc gagaagccga tggcactgaa catcgccgat
     9121 tgcgaccgga tgatcgccgc cgcacagaac gcaggcgtga aactgatggt cggtcaggtg
     9181 ttgcgcctca tccccggctt tgccaaggcg cgagaactgg tcgagtcggg agagtttggc
     9241 aagccgctgg ccatcgccat cgagcgctcc tccttctgga agccgcgggc atggcgccgg
     9301 gagtatcggc cgacgggcgg cgtcctcttc gagatcaacg cccacgaaat cgactacatg
     9361 cgcgccatcc tgggagacgc agccagcgtc tacgcgtgta tctccacgga gacgtgtcct
     9421 gactcggata ttcctgggat caactacgtg ctggtgcgct tcaaagaggg cggcatcggt
     9481 atgctcaact ccaactcgct cattccccag ggccaatacc aggtggcgat cacgcttgaa
     9541 aaggggagca ttcggtgcga ttggagcagc gtcacctacc agttccgtga ccgcgaagct
     9601 caggcggtgc cggaggagga gatgaaggcg atgctcccgg gtgtccagcg cgagatcgac
     9661 tccttcgtcg agtgggtcct ctacggcaag gagccgctgg tcacggcact ggacggccgc
     9721 agcgccgtcg agatcgctca ggcggcgctc caatcgggcg ccaccggcca gccggtctcg
     9781 ctcccgctgc aatcgtcctg atcgcagctt gcgcatcact gtttgggtgg gggcgcgagc
     9841 cagttggccc gcgcgcgtgg gtatctctac ccccgcgcca tgacggcttg cgctccccct
     9901 ccccgcgcgt ccgagagtgg attcggcgtg tgggatctct tgccagcaga ccttccctct
     9961 gctataatga ggatgtcggc gtctatgatc gccggcaagc gtgcccccgt agctcagtgg
    10021 agagagcaac tgccttctaa gcagtgggtc gcccgttcga gtcgggccgg gggcgccact
    10081 tttcctccag ggctcgcttc ctcacgcccc aacagggaac cagcgccgca tcacgcacac
    10141 tcttgaaagc gtatgcgctc gaaagttcgt gtggcactca tcctcatcgc cggcatactg
    10201 gttgttgccg ccgggtgccg gcgccagacg ccgcccaaga agggcaagac cccgccagct
    10261 cccaagacgg tggatgcccc gcgggcgccc ctgcccccca ctccgccggg catggccgta
    10321 tcggggttct gggccacctg gggccgtccc ttgcaccgct gcctggagga gcaccgcgcg
    10381 cgcagcggca ggcagtgtca cggcgctctc tccatcgtca tcgaaaagag caagcgccgg
    10441 atggtcgtcc ggtgcgatgg cgagccggtg aagggctatt tcatcggact gggccgaaac
    10501 cccaaagggg ccaaggagcg ccgcggcgat ggacgcacgc ccgcgggcca ctactacctg
    10561 tggcgcaaga atcggcaatc gcgcttctat ctctcgcttg gtgtctctta ccccaaccgg
    10621 caggatgccg accgtggcct ggccctcggg attatcgacc agaagacgca cgcccgcatc
    10681 tgccgggcgc tccagcgcgg cagcccgccc ccccaggata cccgcctcgg cggcgacatc
    10741 tacatccacg gcggcggcgt cggcaagatc gtccgcatgg acgggaagcg cttcgtgcac
    10801 gtgcgcgact ggaccgaggg gtgcgtggcg ctccgcaaca gcgatatgga ggagctgatg
    10861 gagctcttgc ccgtcggcac ccctatcgag attcgggagt gaggatgcgc ccgcaattcg
    10921 aaggagggcg ccgcagcccg ccctctcccg cacctgcgac cggtgccctt cgagctgggg
    10981 ggccttcagg gaaagcgctg acggaaacgc cgtccttctc ccgatagacg cgggcaaaga
    11041 gcggggttac aatgaggcgt aagccgaggg cttgcatccg tccgcgcgaa cactccgcgc
    11101 cggatgcccg cgcgtggcgg tttcggctgg cagcaagggc gccgaaaagg aaagggaaga
    11161 gaagatgcca gcattgtccc aagtggtgga aaagagcctg tgtcgggcct gctccggacg
    11221 tctcgtcgtc tccgtggcgg agggcgagcg gctgcaagag tgcccgcagg atgaggagcg
    11281 ccaggcggag tgcctggcac ggcttatgta ccgcctggcc tgcctcgacg aggcgctgcg
    11341 ctactatgca agcggcaaag ttgccggcac ccctgaactg cgtgacatca tcgacttctg
    11401 ggcaaaggaa gcgatggggc cggaagccga ccccaatcgc ctctgcttcc gcgatattct
    11461 cctccgccag cggtaggagc tcgtctcaaa ccgccacaga ggacgcagct ccgatttctg
    11521 ccgagacgat ctgcgccctc tgtggcgggg caacgctccc tctcagccgc cgatgggaat
    11581 ctcgacggcg gcagtgccgg ccttgacagc gctcgccacc agccgggcgg tgccacggcc
    11641 cttcacgaac cactccaggt cgcgcttgcc ggaccgcgcg ccgaggcgac ccagctccac
    11701 tcccgcttcc cgcgagagca tctccatccc atcgcccaac tccaaccggg ccataggcga
    11761 ccgattggcg cgcacatccc gccccgtttc agcgatgtag gtggggaagc cgcccgtgtt
    11821 catcacggtg gcccggattc ggaagatatc gctgcccacg gcatccacgc gaacgcccac
    11881 gatccgcagg tggggatagc gtcctgcgtg ctccaggatg aagcgcgtgc atccctcggc
    11941 gatgcccggc aactcgcgcg ggtccgggtt ggcgaaggca ttcatgcgca gcccgccgat
    12001 ctccacccgg cccaactgag ggtgctcgaa gggctgccag tcgacgaagg cgccggcctc
    12061 ggggtgggca tcgtgccagc gaaggatcgc ccggtcgaac tcgcggcgct cttcatcgct
    12121 cttgctggcg aagtactggt ccgtcgtaat gccggcggac tgatagagca tgcccaattc
    12181 gatctcgaag gggtagatgc cgaggtgctc gtaggcccaa tcggtgaagt ggccctgaag
    12241 ccggttcggc cggctctcat cgaggcggta ctgagtcacg gcgcgcagct tgaacccggt
    12301 tgcctgctcg cccttgcggc ccagctcgcg caacaccgcc aggtcgcggt cgttgagctc
    12361 ctcatccgga cgggaggccg ggggccggag gacgccgttg cacccggtgt ggaagccgag
    12421 cgcgccaaag atgttggggt gcgcggcaac aaactccgca acggcccgca tctccggctc
    12481 agagaagggg aagtcgccgg cgccatactg ctggtgctcc gggcgccagt tggcacccca
    12541 gttgcggttg aaatctttgt ggtagctcgc ctcgcgcggc tcgccgccat cccaatcctc
    12601 gatgaatccc tcagtgaaga cgcggtagaa cggcccctcg tcgtcgtgcg gctcccgggg
    12661 caccatcagg cgcgggtcct gccgatccgg gcgctggtca ccatcctcgc agcggatgcg
    12721 cattcgcagg atgaggccat cgccgttcac atctgcgggt cggatcgcgt tgcgcgccgg
    12781 cactggcacg agccggctgc ggatcgcgcc ccccgtggtg agcgcatact cggcgccatc
    12841 ggggttgatg cgcggcacga tgtagaacac cacttcgccc agaagccgct gcagctcggg
    12901 cgtgtcgtcg gtgaccaggt agtgggccac gtagagcgcc gccgtcgtac cggagagctc
    12961 ctgggcatgg aggttgccgt ggatcaggta ggcgggcttg cgatcgggat cgccccgggt
    13021 gaaatcggtc accgtcagca tccacagctc gcgtccttcg ggcgagcgac caatcgagtg
    13081 gcgccggacg cgatcgggcg cgaaagccgc cagctgctcc agggctcgcg tgagctcctg
    13141 gtagctatag aagcgatcga agcgaaggtc gagagatgcc ggcatccttt gtctccttgc
    13201 aaagccgtga tgggtgctgc tccatgaaag gtgcccaggc gcgcttccag gaggcttccc
    13261 tgcccgtcca gcaccccatg gaggcagcct tccgcactct gcgttcgggt gcccgcaggg
    13321 gctctcctgc ccaggccccc aatatgaagc gccgcgcggg acgcccgaaa tgcccatcga
    13381 ttgattgaca tcgtcatgct ctccctgtta taatagggct acaccaatac ggagagatcg
    13441 cctagtctgg tttagggcgc cgcattggag gtgcggtgtg ggggcaaccc caccgtgggt
    13501 tcgaatccca ctctctccgc caaaaaaagc ccggcttgcg cgaagagggc gtgaagccag
    13561 gggccagttg gtcgtgctag acggggaact agcggtgccc tgtaacccgc aatccgctat
    13621 agcggggtcg aactcccggc ctgagggtcc ggcctgtggg gtctgacccg tgtaagcggt
    13681 gtggatgact gggtctcgcg caacattccg gtatcgaatc ccgtcaggtc cggaaggaag
    13741 cagcggtaag gtatcagggg tgtgtgccgc gaggaagcct ggtcggagct ggctgctcgg
    13801 gtaacgcctg gaggccgcga ttcgagggcg ggtgcacgat cactttttgg ggccaagagc
    13861 attctcgatc tcgtcttttg gcttgcgccg ccgggatgat cggggggctc ttggccctac
    13921 ctcttttcgg ggacgccgac gacaaggagg atgtactcgg gaggagtgga gtacgcgctt
    13981 gccaggcagg tgtctctctc ccactccccc acgccgtgat tccagcacgc tgctgccagc
    14041 cgttgggttt ccgagacatc agggtggtcc gggatgtcca cgtacatttc gctctatcgc
    14101 aagtaccgac cgcagacgtt cgccgacgtg gtggggcagg cacacatcac gcgcaccctg
    14161 cagaacgccc tcgaaggtgg ccgcgtcgcg cacgcctacc tcttctgcgg cccccgcggc
    14221 accggcaaga cgaccaccgc tcgcctcctg gctaaggccc tcaactgcag ccgcggtccc
    14281 acgcgcgatc cctgtaacga gtgtgaatcg tgccggagga tcgccgcagg caccgcgctc
    14341 gatgtgatcg agatcgacgc ggcatccaat cgcggcatcg atgacatccg ggaactgcgc
    14401 gacaaggtga aatacgcccc ggcggaagcg cggcacaagg tctatgtcgt cgacgagtgc
    14461 cacatgctca ccacggaggc gttcaacgcg ctgctg
//
LOCUS       DAOSCV010000004        13942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_2967_length_13942_cov_3.446749, whole genome shotgun sequence.
ACCESSION   DAOSCV010000004 DAOSCV010000000
VERSION     DAOSCV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13942
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2967_length_13942_cov_3.446749"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="PLY56_00235"
     CDS             <1..1775
                     /locus_tag="PLY56_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19938.1"
                     /translation="MLRVFAQEHLPALGDERSMIRASEEVDAFCRFAVLALQNAQRQS
                     IRSRQNGTRALIAALYEIDNRRVLLRHLLRGIVSDAACEEHLIASGLLAPVQLELVCD
                     ARQAIQELPVEPGQLDDLLRAAFVESHAGAGADEEQRRRVWALCDLAASLFGSQEEPG
                     PASALLFDLLAPLTPVLFAREHSHPILSADVFDVFVETLRGRSEAILHYLSQRPADSA
                     LSDAVFLALILRSMEVRRSSRIPLWDALTSALRSGEKLGGQRADDLIHLSDAAEAERL
                     FERLRSGDLSSDGRLITRLCDDVAKELGIDFRAASLEELAYARDDILAILHRNLTRLC
                     ERLQPDRDLLLRLAAGTLSEEEKQSLLKSAATPRKSGGRHARRPRHASSPQEAPSDLE
                     ALRARLTENARRYAEYEHLATALAPYAEDTTASLYDALFAALAGRPAEVVRPPLSVLT
                     PLVQHAPSGEEATSEKAAPAPEQADADEEPERKPVGPVDEFLREFDHLGSDALAADLV
                     EATLVLFGNDERVRETLSRNRKQVLDAILAREFPGVMKSTRMQIVVCAEPRGLRLHDI
                     SDLCKMVSEGGVSPTEVRNLLQSM"
     gene            2045..4882
                     /locus_tag="PLY56_00240"
     CDS             2045..4882
                     /locus_tag="PLY56_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOJ19939.1"
                     /translation="MLLGLLAVSALILGAAPLAAAPAAVVGDLRATLTPFDGVTPGAG
                     LRVTYRGLPLITRSSINVVSPDWKKTHLTLGRSRAKVVASDLPGGKRLALSAANQAFE
                     LEYAIDILEGNRLILDLRGVLHADEPAIIEYSAGYLSAVLLAGQPYRAVVAEGERSGL
                     LPYTGSGTDPRSALVLSPFRRLRVDSRVGTITVEEERAHQGLDLFDARKVPSPWLQAS
                     PTLWLGYLEVPLEKGKPFHSRVTLTIDPVEMAERPDRSSPSVKHLPVRDVPDARLSSD
                     PDGRLIPTPRQVRPGVGSFALDEQTRIVVGEDAASRRAARELQERVRERFGLRLRTAP
                     VSLWREEGNVIVVGTRTSPAVRKWLARAGLREVTERDGYALRVTPDYVVIAGADAPGT
                     LYGAFTLLQTIHPDTAAPARIAATTIRDWPALATRGVHLLTDHWSTFLHRDLIRDVLA
                     PHKVNTIVLECEYVKWESHPELHASWGMPKEEVRALLALARDYYIEVIPLVQTLGHAN
                     WIFHNNQHLDLAEDPKAKYAYCPSNPKTYDLVFDIFEEALELFGNPRYFHIGHDEFHH
                     RGEFAQHEPCKSKGVDQLFIEDTRKLHNFLAERGARTLMWGDMLLGPGEGGDATNAKS
                     VAEARRRREQLPKEILITDWHYAVADDYPSIRILREAGFEVWAATWYEPENVRGFARA
                     AAAHGSGLIQTTWTGYYGNMTALRREFPQFRAYLLAAGYAWNPESPALAEATEAAAQR
                     FLASWPAFSRAPGKEPGFVVDLAPLGNMPWCDTPSRDGWLGYGPDHDLSALEGGVRRL
                     GHSLFHLPGNSRAVALHGPLLPMRYPNSVTIPLGRRASELLFLMATGWEVAEGNPVGK
                     IVVRYADGTQMPSTLLYGAHLRAAHDPRTAVYAPVAWQGRTTGGEPLYLRVYTLRNPF
                     PEREITEVTLVADDTAASPVLFALSGVAPER"
     gene            5113..5814
                     /locus_tag="PLY56_00245"
     CDS             5113..5814
                     /locus_tag="PLY56_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ19940.1"
                     /translation="MTDSVLDIEVVRENSSCPVIRLEGELDASTAPELKSTVLALVTE
                     GFRDIVIDMERLYLLDSLALGVLMSACQRVPGKLTIQNPTPPVRRVLDAGGVSRLIEI
                     RDAAGEQQQLLNFGITIEDTAEGIPVVYLRGELDAYSAAHFRGALISLTQEGHRFVVL
                     HLGALDFIDSVGLGSMVSIYTRLKKAGGCFYIAAPSEQISKVLHITGLDALFPLYPSD
                     AEAMAAAVRVAAGSA"
     gene            5830..7518
                     /locus_tag="PLY56_00250"
     CDS             5830..7518
                     /locus_tag="PLY56_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOJ19941.1"
                     /translation="MDASAQARILVADDSPLILDMVRQVLEAAGHTVLTAPDGLAAIN
                     MVLRERPDLIISDVEMPEMTGLQLCRLLKGDSRTRGIPFLILSAHKEQYEQFWGRESG
                     ADDYLPKPFGPGALLERVQALLARFPRSSETGKAIELPITTRIEALERVNASLERRLF
                     EQTIIHGISAIAATASGERDTARAVLERLSRLVELSAGAFAFAKGGTSYVLVRGPVHP
                     RTLQQLHAAIVSRLQLSGSELAAPVLVAGAEYISERHPNPEPAIAVQVLQAAGHPVGF
                     LAVARTSGRDFDEPERELLELVAGQAALVLENARLVEAERAHAQALAEQNEELRRLNR
                     TITDLIATVTHDLRTPLSSVIGYLELALEENGIPEETAEFIRIARRNGERILQMINDL
                     LDLSRLEAGRTPIAPSPVSLAPVLREVVTLLRPDLEAKRQVYTEEIPADLPALMADPD
                     RLHQVLLNLLGNASKYTPEGGRIHASARVEGDRVRIAIQDTGMGLSKEDLRQVFGKFF
                     RAQRPEMTGVTGTGLGLAIVKTVVELHGGEVFAESRLNEGSTFGCLLPIARAPR"
     gene            complement(7572..12404)
                     /locus_tag="PLY56_00255"
     CDS             complement(7572..12404)
                     /locus_tag="PLY56_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HOJ19942.1"
                     /translation="MAEPAPDGEAAPTENPAARLERWKSRLLDLTLRNRLLNFKETKK
                     SLPILCPDLAALEDALAEGERFSILPLPLELQEGQPRAAAIHRWRTGKDLNIELLREE
                     LKAHRLRTHHDEAEMKRRLLEIYREARTSLEENGANTLYLALGFLKWRETPGADRTYF
                     APLLLLPLEIARRSIQEGFSITRGDDDPLVNTTLLELLARSFDIKVPGLDPLPQDEKG
                     LDVPAILHRFREAVLHIDGWEVVEVACIGHFSFTKFLMWRDLEVRADDLQQNQIVSHL
                     INTPTEPFPYEGEFPNPERLDETHSPLETFCPLSSDSSQLAAVYAAAEEKSFVLHGPP
                     GTGKSQTITNLIAHALAMGKSVLFVSEKAAALNVVHERLKKCGLGPFCLALHSNKSHK
                     VQVIRQLYETMECFAARTEDEWRREAARLAELRRELNDYAGALHRVRETGESAFQGMS
                     RLIGLRDAPRVDLSWQRDRRLDRETRDQMREIARQLEGAARGSAHPSNNVWGPVECDE
                     WSHAWRSDVEAAIQALQAASGELQRAAEAAAPLLHLSSSGWSEEDLENTFSVAKALLE
                     SPEPPAALVVEPDWDALNESVNSWLEHGRRRDALREQLYARYTEGILQTDLDSLWQRL
                     ASAEASWALPRALGRWQVRRALTRVAQSGQPVAAESLKGDLLAARSLRDEENAVAEAG
                     DRARVLLGRFWREGEADWEEIARVRDWTASLRSLATRIAGTDLERAATLRQEWARLVE
                     EGREQIRSDGPIGRRLQDFLQAARQFAVARQRLNTVLHLNTMAAWGARSDPDLLKTVD
                     ARLNDWLEHLDQLRDWCHWQSVRGQAVTAGLQGVVTAYEQGQIAADQISKAFERSATQ
                     WWVEGVIDSEPTLRGFHRQDFERKIRDFAAIDERYTALTRSVIQARLAAQVPRPGDRM
                     SSNSEVGILMHQNRRQRGHMPIRTLFQKIPNLLPRLAPCLLMSPLSVAQYLDPAHPPF
                     DLVVFDEASQIPVWDAVGAIARGKEAIIVGDPKQLPPTNFFNRAEDDETSDESVVEDL
                     ESILETCIAAQLPELRLRWHYRSRHESLIAFSNYHYYSNDLLTFPSPHTDTALSLRPV
                     SGHYDRSKARTNRAEADAVVAEIIARLTSPEHCHASIGVVTFSMAQQTLIEDLLEDAR
                     RQHPEIEPFFNGGDGVSEPVFVKNLENVQGDERDVILFSVCYGPDAQGRVSMNFGPLN
                     RDGGERRLNVAITRARQEIVVFSTLRADQIDLSRTRAQGVADLKCFLDYAARGPRALS
                     ERREVDPGAECESPFEQQVCNALRERGYMVHPQVGCSRYRIDLGVVDPEHPGRYLLGI
                     ECDGATYHSAHTARDRDRLREAVLRDLGWRLHRVWSTDWWERPAEELARIEAAIEDAK
                     KQRRQPAPPPPPIIPAAPLVAPPAAPKSEPRPAPTAPKPEPRRAPEIPVYVPYQVDQE
                     LGDLNAFLDSRSTQEILERLEAVIEAEGPVSLDLAARRVAACWGIRRVGHRVEERIKK
                     LAPRARCRRTPHGEKAFLWPEGLDPAEYTGFRAAGEDPDAQRGISDIPPEEIANAALH
                     VLKGQVSMPADDLIQQTGRALGFQRVGRNVREGIRAGIDLLCERGDACEEDGSVTLSK
                     "
     gene            complement(12582..13541)
                     /locus_tag="PLY56_00260"
     CDS             complement(12582..13541)
                     /locus_tag="PLY56_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19943.1"
                     /translation="MQARDSQGVPPQLPAGDWGTGPAPAVTVELETTRVVNYAMQQND
                     VPVVTRLRIANAGDASLRDLHVRITTDPGFSAPWELHLAELRPGEARDLGVVDLPLSH
                     SFLAQQTERVAGALQVEVAQGERSLACHHQRLDVLAYDEWSGSQAPPEMLASFVTPNH
                     PAVEAVLADAAALLGEWTGDTSLSGYQSGDRSRVLLTVGAIYAALQGRGIRYSEPPAS
                     FEDAGQKIRLPDRILEHRLGTCLDLAVLSAACLEQAGIHPLVVIIAGHAFPGAWLVDD
                     CFADCAMDDAVHLRKRVDVGELCVFEATALTRSPACPVERALE"
     gene            complement(13739..>13942)
                     /locus_tag="PLY56_00265"
     CDS             complement(13739..>13942)
                     /locus_tag="PLY56_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HOJ19944.1"
                     /translation="EVHREVLHHIADVAEGLGHRVAGLIPSPITGTEGNIEFLIHLIP
                     GQGPRPDIDQVVDQAHARANHPS"
BASE COUNT         2283 a         4670 c         4532 g         2457 t
ORIGIN      
        1 agatgctgcg cgtctttgcc caggagcatc tgcctgccct gggtgacgag cgctcgatga
       61 ttcgggcaag cgaggaggtg gatgccttct gtcgcttcgc cgtgctagcc ttgcagaacg
      121 cccagcggca gagcatccgc agccggcaga acggcacccg cgctttgatt gccgcgcttt
      181 atgagatcga caatcggcga gtgcttctcc gacatctcct ccgtgggatt gtcagtgatg
      241 ccgcatgtga ggagcacctg atagcgagcg ggctgctcgc gccggtccag ttggagcttg
      301 tgtgcgatgc gcggcaggcc attcaagagc tgccggtgga gccggggcaa ctggacgatc
      361 tccttcgtgc cgcgttcgtt gagtctcacg ccggtgccgg ggctgacgag gagcagcgtc
      421 gccgcgtgtg ggcgttgtgc gacctggccg cctcgctttt cggcagccag gaggagcccg
      481 ggcccgcttc ggcgctcctg ttcgatctcc tcgcgccgct cacacccgtg ctgttcgcgc
      541 gcgagcactc gcatccgatc ctctcggcgg atgtcttcga tgtctttgtc gaaaccctcc
      601 gcggtcggag cgaagcgatc ctccactacc tcagccagcg gcccgctgat tccgcgctct
      661 cggacgcggt cttcctcgcc ttgatcttgc ggagcatgga ggtccgccgg tcgtcgcgga
      721 ttccgctgtg ggatgccctc acgagcgcct tgcgtagcgg cgagaagcta ggcggccagc
      781 gcgccgacga cctgatccac ctgagcgacg ccgccgaggc cgagcgcctc ttcgagcgtc
      841 tgcgatctgg cgatctgtca tccgatgggc gtctgatcac ccggctctgc gacgatgtgg
      901 cgaaggagct ggggatcgac ttccgcgcgg cttccctgga ggagctggcg tacgcgcgtg
      961 acgacatcct ggccatcctg caccgcaatc tgactcgcct ctgcgagcgg cttcagcccg
     1021 accgtgacct gctccttcgc ctcgcagccg gcacgctctc cgaggaggag aagcagtcgc
     1081 tgctgaaatc ggctgccact ccgcgaaaga gcggcggccg gcacgcccgc cggccacggc
     1141 acgccagtag cccccaagag gctccatcag acctggaggc actccgcgcc cggctcaccg
     1201 agaacgcccg ccgctacgcg gagtatgagc accttgccac cgcattggcg ccctacgcgg
     1261 aggataccac cgcgtctctc tacgatgccc tgtttgcggc cctcgccggg cgcccggccg
     1321 aagtggtgcg tcctcccctc tcggtgctga ctccgctcgt gcaacacgcg ccctcaggcg
     1381 aagaagcgac gagcgagaag gcagcccctg ctccagagca ggccgatgcc gacgaggagc
     1441 ccgaacgcaa gcctgttgga ccggtggatg agttcctgcg agagttcgat catctgggca
     1501 gcgatgccct tgccgccgac ctcgtggagg ccacgctcgt gctctttggg aatgacgagc
     1561 gcgtgcgcga gacgctttcg cgcaaccgaa agcaggtcct cgatgccatc ctggctcgcg
     1621 agtttcccgg cgtgatgaaa agcacccgga tgcagatcgt ggtctgcgcc gagccgcggg
     1681 gtctccgtct ccacgacatc agcgacctgt gcaagatggt gagtgagggg ggcgtcagtc
     1741 caaccgaggt gcgaaacctc ctccaatcga tgtgacactc gcacggacgc ttgctcggag
     1801 cgccgtctac catcggtagc tcgatgccgc gggtggagac gcgacccgag ggggggtgtt
     1861 gagcgtgccg ccggggagcc aatgcccagg atcgtgcgct ctccctctgg ctcactgttc
     1921 tcgcgtccag agctgtcacg ctcaggaatg ccggtttgac ctggctttcc cgcgatgata
     1981 taatcgctct atggtcgcat tccgaagttc tctcaccagc gcgagcatca ctcgcgcgag
     2041 cgccttgctt ctcggtctgc tggccgtttc ggcactgatc ctgggggcag cgccgttggc
     2101 tgccgcgccc gcggctgttg tgggcgatct gcgcgccacg ctcaccccgt ttgatggggt
     2161 cacgcccggc gccggtctgc gcgtcaccta tcgcggcctg cctttgatca cccggtcgtc
     2221 catcaacgtg gtctccccgg actggaagaa gacgcacctc actctggggc gttcgcgcgc
     2281 gaaggtggtg gcatcggacc tacctggggg gaagcgactg gcgctctctg ccgccaacca
     2341 ggcgttcgag ctcgagtacg ccatcgacat cctggaggga aaccgcctca tcctcgacct
     2401 gcggggcgtt cttcacgcgg atgagccggc catcatcgag tactcggcag gctatctctc
     2461 cgcggtgcta ctcgccggac agccgtatcg cgcggttgtc gccgaaggcg agcgctcggg
     2521 gctacttccc tacaccggat ccggcacgga cccccgttcg gcgttggtgc tctccccctt
     2581 ccgccgcctg cgcgtcgact cgcgcgttgg caccatcaca gtggaagagg agcgcgccca
     2641 tcaggggctg gacctctttg atgcgcgcaa agtgccctcg ccctggctcc aggcgagccc
     2701 gaccctctgg ctcggctatc tggaggtacc cctggagaag ggcaagccgt ttcactcgcg
     2761 cgtgaccttg acgatcgacc cggtggagat ggcggagcgg ccggatcgct ccagtccgtc
     2821 ggtgaagcac ctgcccgtgc gcgatgttcc ggacgctcgg ctctcttccg atccagatgg
     2881 ccgcctgatc cccactccgc gccaggtgcg ccccggagtc ggaagcttcg ccctcgatga
     2941 gcagacgcgc atcgtcgtcg gcgaggatgc cgccagccgt cgggcggcgc gcgagttgca
     3001 ggagcgtgtg agagagcgct tcggcctgcg cctgcgcacg gcgccggtct cactctggcg
     3061 cgaagagggc aacgtcatcg tcgtcggaac gcgaacctcg ccggcggtgc gcaagtggct
     3121 cgcccgggcc ggcctgcgcg aggtaaccga gcgtgacggg tacgcgctgc gcgtaacgcc
     3181 ggactacgtg gtgatcgctg gcgccgatgc gccgggcacg ctctatggtg ccttcaccct
     3241 gctccagaca atccacccgg atacggcggc cccggctcgc atcgccgcaa ccactatccg
     3301 cgactggccc gccctggcga cacgcggggt ccacctcctc actgatcact ggtctacctt
     3361 cctgcatcgc gacctgattc gcgacgtgct cgcgccacat aaagtgaaca ccatcgtgct
     3421 cgaatgcgag tacgtgaagt gggagagcca cccggagcta cacgcctcgt ggggcatgcc
     3481 aaaggaggaa gtgcgcgcgc tgctcgccct ggcgcgcgac tactacatcg aggtgatccc
     3541 gctggtgcag acgctggggc atgccaactg gatcttccac aacaaccagc acctggatct
     3601 ggcagaggac cccaaggcga agtacgccta ctgcccctcg aacccgaaga cgtacgactt
     3661 agtgttcgac atctttgaag aggcgttgga gctgttcgga aacccgcgct acttccacat
     3721 cgggcatgat gagttccatc accgtggcga gtttgcgcag cacgaaccgt gcaagagcaa
     3781 aggggtggat cagctcttca tcgaggatac ccgaaagctg cacaacttcc tggcggagcg
     3841 cggcgcgcgc acgctgatgt ggggcgacat gctcctcggg ccgggcgaag gcggagacgc
     3901 gacgaacgcg aagagcgtgg ccgaggcccg gcggcggcgc gagcaactcc ccaaggagat
     3961 cctcatcacc gactggcact acgccgttgc cgatgactat ccgagtatcc ggatcctgcg
     4021 tgaggccggc tttgaggtct gggccgccac ctggtacgag ccggagaacg tgcgcggttt
     4081 cgcgcgcgcc gcggcagcgc acggctcggg cctgatccag acgacgtgga cgggctatta
     4141 cgggaacatg accgccctcc ggcgggagtt cccccagttt cgggcctacc tgctggcagc
     4201 gggctacgcg tggaaccccg agtcacccgc gctcgcggag gccacggaag cggccgcgca
     4261 acgcttccta gcctcgtggc cggctttctc ccgagcgccc gggaaagagc ccggcttcgt
     4321 ggtagacctg gcgcccctgg gcaatatgcc ctggtgtgat acgccctcgc gcgacggatg
     4381 gcttggttac ggtcccgatc atgacctctc cgcgctggaa gggggagtcc gacgcctggg
     4441 gcactccctc ttccacctgc cggggaatag ccgggccgtt gcgctgcatg gccccctcct
     4501 gccgatgcgc tatcccaact ctgtcaccat tccgcttggc cggcgcgcgt cggagcttct
     4561 tttcctgatg gccaccggat gggaggtcgc cgagggaaat ccggtcggga agatcgtcgt
     4621 gcgctacgcg gatggcacac agatgccctc caccttgctc tacggggcac atcttcgcgc
     4681 cgcgcatgat ccgcgaaccg cggtctatgc gccggtggcg tggcaaggcc gcaccacggg
     4741 aggcgagcca ctctacttgc gtgtctacac actgcgaaac ccgttcccgg agagggagat
     4801 caccgaggtg accctggttg ccgacgacac cgcggcatca ccggtgcttt tcgcgctgag
     4861 cggcgtggcc ccggaacgct gagcacgagt ccgcaggggc actccttccc cccggtggag
     4921 tgcccccggg gatgcatcgc gcaaggatct ccggcgctcg atggccaata cagttttgga
     4981 tgagtcttgc gagggtagca tatctatggc gggatcgcag cacgcctgcc cctctcaata
     5041 cactcgcgct gcatcctgtc agctggatct gcttcctcgg caacaggaat cgcgtggggg
     5101 aagggcaagc gtatgacgga ttccgttcta gatattgagg tagtacggga gaactcctct
     5161 tgtccggtca tccggcttga gggcgagttg gatgcctcga cagctcccga gctcaagagc
     5221 accgtgctgg ccctcgtcac agagggattc cgcgacatcg tcatcgatat ggagcgcctc
     5281 tacctgctgg atagcctggc gctcggggtg ctgatgagcg cgtgccagcg cgtgcctggc
     5341 aagctgacga tccagaaccc gactccaccg gtgaggcgcg tcctcgatgc gggcggcgtg
     5401 tcgcggctca ttgagatacg ggatgcagcg ggagagcaac agcagctcct gaactttggg
     5461 atcacgattg aagatactgc cgaaggtatc cccgtggtct atttgcgcgg cgagctagac
     5521 gcctacagcg ccgcccactt tcgcggggca ttgattagct tgactcaaga gggccaccgt
     5581 ttcgtggtgc tccacctcgg agcgctcgac ttcatcgata gcgttggttt gggaagcatg
     5641 gttagcatct atacacgcct gaagaaagcg ggcggctgct tctacatcgc tgcgcccagc
     5701 gagcagatct ccaaagttct ccacattact ggcctcgatg ccctcttccc gctctatccc
     5761 agcgacgcgg aggcaatggc ggccgcggta cgagtcgcgg ccggctctgc atagcgccgg
     5821 agcgacgcaa tggatgcatc agcgcaggcg cgcatcctgg tggcggatga tagtcccctg
     5881 atactggaca tggtccgcca ggtcttagag gccgccgggc acaccgtcct gaccgcgccc
     5941 gatggcctcg ccgccatcaa tatggtcctc cgtgagcgcc cggatctgat catctccgac
     6001 gtggagatgc cggagatgac cgggctgcag ctgtgccgtc tactcaaggg agattcgcgc
     6061 acccgcggca ttcccttcct gatcctgagc gcgcacaagg agcagtacga gcagttctgg
     6121 ggccgcgaga gcggcgccga cgattacctg cccaagccat ttgggccggg ggcgcttctg
     6181 gagcgggttc aggcgcttct ggcgcgcttc ccacgctcct cggagactgg gaaggcgata
     6241 gagcttccga tcacgacgcg gatcgaggcg ctggagaggg tgaacgcctc tctggagcgc
     6301 cggctcttcg agcagacgat tatccatggc atcagcgcca tcgccgcaac cgcgagcggt
     6361 gagcgtgaca cggcgcgcgc ggtgctcgag cggctttccc gactggtgga actaagcgcc
     6421 ggcgccttcg ccttcgcgaa agggggtacc tcctacgtcc tcgtccgcgg cccggtgcac
     6481 ccgcggacgc ttcagcagct tcacgcggcg atcgtaagcc gtctccagct ttctggtagc
     6541 gagctggcgg cgcccgtcct cgtggccggc gccgagtaca tctcggagcg gcatcccaac
     6601 cccgaaccag ctatcgcagt gcaggtgctt caggccgcgg gtcaccccgt gggcttcctg
     6661 gccgtggccc gcacctccgg gcgcgacttc gacgagccag agcgagagct cctcgaactt
     6721 gtcgccggtc aggccgcgct ggtcctggag aacgcgcgcc tagtggaggc cgagcgcgcc
     6781 cacgcacagg cgcttgccga acagaacgag gagctacgcc ggctcaaccg caccatcacc
     6841 gatctgatcg cgaccgtgac gcacgacctg cgcaccccgc tcagctcggt gattggctac
     6901 ctggaactcg cccttgagga gaacggtatc ccggaagaga ccgccgagtt catccggatc
     6961 gcacgccgga atggcgagcg gattttgcag atgatcaacg acctccttga ccttagccgg
     7021 ctggaggccg ggcgaacccc cattgccccc agcccggtgt cgctggcgcc cgttctgaga
     7081 gaggtggtca cgctcctccg ccccgatctg gaggccaagc gccaggtcta caccgaggag
     7141 atccccgcgg atctgccggc gctgatggcc gaccccgacc ggctccacca ggtgctcctc
     7201 aacctgctcg gcaacgcgtc gaagtacacc cccgaggggg ggcggattca cgccagcgcg
     7261 cgggtggagg gcgaccgcgt gcggatcgcc atccaggata cgggcatggg cctctccaag
     7321 gaggatctgc gccaggtctt cggcaagttc ttccgcgccc aacgccccga aatgaccggg
     7381 gtcacgggca ccgggctggg tctcgccatc gtgaagacgg tcgtcgagct tcacggcggc
     7441 gaggtgtttg cagagagccg gctgaacgag gggagcacct tcggctgcct cctccccatc
     7501 gcccgcgcgc cccgctgagc cagccgccct gctatcagcc agcgccgcgg catcccagag
     7561 gcagcacccg ctcatttgga gagcgtgacc gacccatcct cctcgcaggc atctccacgc
     7621 tcgcagagga gatcgattcc ggctcggata ccctctctga catttcggcc cacgcgctgg
     7681 aaccccagcg cccggcccgt ttgctggatc aggtcgtcag ccggcatgct cacctgcccc
     7741 ttcaggacgt gcaaggccgc gttggcaatc tcctcgggcg ggatgtcact gatgcctcgc
     7801 tgcgcgtcgg gatcttctcc ggcagcccgg aagccggtgt actccgccgg gtccagaccc
     7861 tcgggccaca ggaacgcctt ctctccatgg ggagtacgcc ggcatctcgc gcggggggcc
     7921 agtttcttga tgcgttcttc gacgcgatga cccacgcgcc ggatacccca gcacgcggcc
     7981 acccgccttg ccgcgagatc caggctgacc ggcccctcgg cttcgatgac cgcttcgagc
     8041 cgctccaaga tctcctgggt ggagcgagag tcgaggaatg cgtttagatc tcccagctcc
     8101 tgatccactt gatacgggac gtagacgggg atctccggcg cgcgccgtgg ctccggcttt
     8161 ggcgccgtcg gcgcaggacg tggctccgac tttggcgccg ccggcggggc gacaagcggc
     8221 gccgcaggaa tgatcggcgg cgggggaggc gcaggttgtc tcctctgttt ctttgcgtcc
     8281 tcgatggccg cctcgatgcg cgccagctcc tcagccggcc tctcccacca gtccgtggac
     8341 cagacccggt gcaggcgcca gcctaggtcg cggaggactg cctcacgcag ccggtcgcgg
     8401 tcgcgagcgg tatgcgcact gtggtaggtg gcgccatcgc actcgatacc gagaagatag
     8461 cggcccgggt gttccggatc caccaccccg aggtcgatcc ggtagcggga acacccaacc
     8521 tgcgggtgta ccatgtagcc acgctctcgc agcgcattgc agacctgctg ctcgaaaggc
     8581 gactcgcact ccgctccggg gtccacctca cgtctctcag acagcgcccg cgggccacgt
     8641 gccgcatagt ccaggaagca cttcaggtcc gctacgccct gggcgcgcgt gcgcgagagg
     8701 tcgatctggt ccgcacgcag agtggagaag accacgatct cctggcgggc acgggtaatc
     8761 gccacgttca gccggcgctc gccgccatcg cggttcagcg ggccgaagtt catggaaacg
     8821 cggccctggg cgtccggccc gtagcagacg gagaagagga tcacatcgcg ctcgtcgccc
     8881 tgcacgttct ccaggttctt cacgaacacc ggctcggaca cgccatcgcc accattgaag
     8941 aacggctcga tctccgggtg ctgccggcgc gcatcctcca ggaggtcctc gatcagtgtc
     9001 tgctgcgcca tgctgaaggt gaccacgccg atgctggcgt ggcaatgctc ggggctggtc
     9061 aaccgggcga tgatttccgc caccaccgcg tcggcctccg cgcggttggt gcgcgccttc
     9121 gaacggtcgt agtggcccga cactggccgc agcgagaggg cggtatcggt gtgtggcgat
     9181 gggaacgtca gcagatcatt gctatagtag tggtagttgc taaacgcaat caagctctcg
     9241 tgccggctgc ggtagtgcca gcgcaggcgc aactccggga gctgggcagc gatgcaggtt
     9301 tcgaggatgc tctccagatc ctccactacg ctctcgtcgg atgtctcatc atcctccgcg
     9361 cggttgaaga aattagtcgg cggcagctgc ttgggatcgc cgacgatgat tgcctctttc
     9421 ccgcgcgcga ttgcccccac cgcatcccag accgggatct gcgaggcttc gtcgaagacc
     9481 accaggtcga acggtgggtg cgcgggatcc aggtactgcg ccacggagag cgggctcatc
     9541 aggaggcagg gcgccaggcg gggcaagagg tttgggatct tctgaaagag cgttcggatg
     9601 ggcatatggc cacgttgccg tcgattctga tgcatcaaga tgcccacctc cgagttgctg
     9661 ctcatccggt ctcccggccg cggcacctgc gccgcaagtc gcgcctggat gacggagcga
     9721 gtgagcgccg tatagcgctc gtcgatggcg gcgaagtctc ggatcttgcg ctcgaaatcc
     9781 tgtcggtgaa agccgcgcag tgtcggctcg gagtcgatca caccctcgac ccaccattgc
     9841 gtcgcgctcc gctcgaatgc cttggagatc tgatccgcgg cgatctgccc ctgctcgtac
     9901 gcggtgacca cgccctgaag ccccgcggtc actgcttggc ctcgcaccga ctgccagtgg
     9961 caccaatctc gcagttgatc cagatgctcc agccagtcat tcagccgagc atcgacggtc
    10021 ttcaggaggt cgggatcgga gcgtgcgccc caggcagcca tcgtattgag gtggagtacg
    10081 gtattcagcc gctggcgcgc cacagcaaat tgtctcgcgg cctggagaaa atcctggagg
    10141 cgcctgccga tagggccatc cgaccggatc tgttctcgcc cttcctcaac caaccgggcc
    10201 cattcttgcc gcaaggtggc cgcacgctcc aggtctgtac cagcgatccg agtggccagg
    10261 gagcgcaggg acgcggtcca atcacgcacc cgggcgatct cttcccagtc tgcctcgcct
    10321 tcccgccaga accgtcccag aaggacgcgg gcgcggtcgc ccgcctcagc gacggcgttc
    10381 tcctcgtcgc gcagggaacg agcggccagc agatccccct tcaggctctc tgccgctacg
    10441 ggctggccag attgggccac gcgcgtcaag gcgcgacgca cctgccaccg gcccaacgcg
    10501 cgcggaagcg cccacgatgc ttccgcgctg gcaagccgct gccacaggga gtccagatca
    10561 gtctgcagga tgccctcggt gtagcgagcg tatagctgct cgcgcagggc gtcacgccgg
    10621 cggccatgct ccaaccagga gttcaccgac tcgttcaagg catcccagtc gggctccacc
    10681 accagcgccg ccggtggctc tggcgactcc aacagcgcct tggcgacgga gaaggtgttc
    10741 tccagatcct cctcgctcca gccgctgcta ctcaggtgta gcaggggggc tgccgcctcg
    10801 gcggcgcgct gcagttcgcc actagcggcc tgtagcgcct ggatggcggc ttccacatcg
    10861 ctgcgccagg cgtggctcca ctcatcgcac tcgaccggcc cccatacgtt attggatgga
    10921 tgagcagagc cccgcgctgc cccctccaat tggcgggcga tctctcgcat ttgatcgcgc
    10981 gtctcgcggt cgagtctccg gtcgcgttgc cagctcagat ccacccgtgg ggcatcgcgc
    11041 agcccgatca gccgcgacat cccctgaaag gcgctctctc cagtctcacg gacccggtga
    11101 agcgctcctg catagtcgtt cagctcgcgg cgcaactccg ccaggcgcgc cgcctcacgc
    11161 cgccactcgt cctcggtgcg tgccgcgaaa cactccattg tctcatagag ctgccgaatc
    11221 acctgcacct tgtggctctt gttggagtgc aacgccaggc agaaggggcc caatccgcac
    11281 ttcttcaacc gctcatgcac cacgttgagc gccgcggcct tctcggagac gaagagcacg
    11341 ctcttcccca tcgcgagcgc gtgcgcgatc aggttggtga tcgtctgcga cttgcccgtt
    11401 ccgggaggcc cgtgcagcac aaagctcttc tcctccgcgg cggcgtacac ggcggcaagc
    11461 tgcgacgagt cggagctgag cgggcaaaac gtctccagag gcgagtgtgt ctcatccagg
    11521 cgctccgggt tgggaaactc gccctcgtat ggaaagggct cggtgggcgt gttgatcagg
    11581 tgggagacga tctggttctg ctgcaggtca tccgcacgca cctcgaggtc acgccacatc
    11641 agaaacttgg tgaaggagaa gtggccaatg caggcaacct cgacaacttc ccaaccgtcg
    11701 atgtgcagga cggcctcccg gaagcggtgc aggatggccg gcacgtcgag gcccttctcg
    11761 tcctgtggta gcgggtcgag acccgggact ttgatgtcga agcttcgcgc gaggagttcc
    11821 aggagcgtgg tgttcacgag cggatcatca tcgccccgcg tgattgaaaa gccctcctgt
    11881 atcgaccggc gggctatctc caaggggagc aagagaagcg gcgcgaagta tgtccggtcg
    11941 gctccgggtg tctctctcca cttgagaaat ccgagcgcca ggtagagcgt gttcgctcca
    12001 ttctcctcca ggctggttcg cgcttctcgg tagatctcca ggaggcgccg tttcatctcg
    12061 gcttcgtcat ggtgcgtgcg caggcgatgc gccttgagct cctcgcgcag cagctcgatg
    12121 ttcagatcct ttcccgttcg ccaacggtga atggccgccg ctcgcggctg gccttcctgc
    12181 aactccagcg gcaagggcag gatgctgaag cgttctcctt ccgccagggc atcttccaag
    12241 gcggccaggt cggggcagag gatcgggagc gacttcttcg tctccttgaa gttgaggagg
    12301 cggttgcgca gggtcagatc gaggagccgg ctcttccagc gctcgagccg ggctgctgga
    12361 ttctcggtcg gagctgcctc gccgtcggga gctggctcgg ccactccggg gagcgccgca
    12421 ctcggtggcc cagagagggt gggggcctcc ggctgttggg gctctgcgcc ctgggtctcg
    12481 gtggaaacac gcaagggcaa ggggcggata tggctctttc gggtgcgaag aatgtcgatg
    12541 gcgtagatga aggcgttcgg gttgtcgagg tgccgccttc cctattccag ggcccgttcc
    12601 acggggcagg cgggggaacg cgtgagtgcg gttgcctcga agacgcacag ctcccccaca
    12661 tccacccgct ttcgcagatg cacggcgtcg tccatggcgc aatccgcgaa gcagtcgtct
    12721 accagccacg cccccggaaa ggcatggccg gcgatgatga cgaccagcgg gtggattccc
    12781 gcctgctcga ggcaggcggc gctcaagact gccagatcga ggcaggtgcc caaccggtgc
    12841 tccaggatgc ggtctggtag ccggatcttc tggcccgcat cctcgaagct cgcgggcggt
    12901 tcgctgtatc ggatcccacg cccctgcagg gcggcataaa tggcccctac cgtgagcagc
    12961 actcggctgc gatcaccgct ctggtagccc gagagagagg tatcgcccgt ccattctccc
    13021 aggagcgccg cggcatcggc cagcacggct tcaacggccg ggtggttggg cgtcacaaag
    13081 gatgcaagca tctccggcgg cgcttgggag ccactccatt cgtcatacgc cagcacatcc
    13141 aggcgctggt gatggcaagc gagggatcgt tctccctggg cgacctcgac ctggagggca
    13201 ccggcgacgc gctcggtctg ttgggctaga aaggagtgcg agagtggcag atccaccacg
    13261 cccagatcgc gggcttcccc aggcctcagt tcggcaagat gcaactccca aggcgccgaa
    13321 aagccggggt cggtggtgat gcgcacgtgc aggtcccgaa ggcttgcatc cccggcattg
    13381 gcgatccgca gccgagtgac caccggcaca tcgttctgct gcattgcata gttgacaact
    13441 cgggtggttt cgagctcaac agtcacggca ggggcgggac cggtccccca gtcgccagcc
    13501 ggcagctgag ggggcacgcc ctggctatct cgagcctgca tacgctcttg tctcccaacc
    13561 gcatcaaagt ccccgccggg cacgcaggcc gggcggaagg ggcgctcgcg cgcggccatc
    13621 ctcaaggcga aagggaaaac ggtcgtgcct gtgggaagac cgcggaaagc cccggttgcc
    13681 tatggtcgtt cgccaggcac acgcgcgatc ccttctcagg ggcactccgc taccgggatc
    13741 aggaggggtg gttggcccgg gcatgggcct ggtccaccac ctggtcgata tccgggcgtg
    13801 ggccctggcc gggaatgagg tggatcagaa actcgatatt gccctcggtg ccggtgatcg
    13861 gcgaggggat gagcccggcc acgcggtgac cgagaccctc ggccacgtcg gcgatgtggt
    13921 ggagcacctc tcggtggacc tc
//
LOCUS       DAOSCV010000005        13643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3051_length_13643_cov_3.649838, whole genome shotgun sequence.
ACCESSION   DAOSCV010000005 DAOSCV010000000
VERSION     DAOSCV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_13643_cov_3.649838"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2563
                     /locus_tag="PLY56_00270"
     CDS             <1..2563
                     /locus_tag="PLY56_00270"
                     /inference="COORDINATES: protein motif:HMM:NF020083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19945.1"
                     /translation="KLPAAVAEGEGIRDALRERLGIIPEVQSNDDGILLRFPDADADF
                     PVDAVVRMSPAEARERLLREIPHSAVFGARFRQNAARALLLPGARPGKRTPFWLQRLR
                     ARELLEAVLRFPEFPILIETFRDCLEDVMDLPHLEEVLRGIQEGRISVVSYSALAPSP
                     IAQGLLAQFISIFMYEGDAPRAEAQLNQLAVNRALLSDVLKDVALDQFLRPEAIDTMV
                     RQLQRIEPGYRARTAEELAVLLEHLGDLTPVEIEARSHSDPGPWVKALAASGRIVSLE
                     VPTAAGSETRWVCAEYASEYARAFALPASPPADEEAVNEARRAILTRALESWGPVTLE
                     AIRTRYAFPSEWLAEALEQLAHEGNLVRGRFTRAAPPGEQFIARRNLEEVHRRTLSLL
                     RREVRPVPLPVYAEFLARWQHAHPATRLRGREGLLQVLQQLRGLPLAAQIWERDVLPA
                     RVDGFSPRDLEALFEEGEVVWVASAQADPRRARVAFFARGEGRVCLPSIEESEELGTE
                     ARAIYDFLKSEGTAVAAEIQSLLGLTAQSVERGLTELATRGLVTCDSLAALRYLLEAP
                     QGSITAAAPERRWQSSLESQLPRRTLGATSWRSRRPSRADYQAAKRRVAERLRQAEPL
                     ALAGRWGLINRLSILGRDLSPDERAARQARLLLQRWGVLSRAIVDAEEGPWEWEGIAA
                     HLRLMEMRGEVRRGLFVDGMQGVQYALPDAVERLRECRDGAPEPDSLVVLNACDPALA
                     YGRAAQEEPISPHRDPLAFPRIPTTWVVQRRGHPLLVAEAGGKEITLLQGADEEDARA
                     ALIILAEHLLRSARRITVHTWNGEPVLESDGKPLLEAAGFYHDYRAMVRESSIGR"
     gene            complement(2570..2986)
                     /locus_tag="PLY56_00275"
     CDS             complement(2570..2986)
                     /locus_tag="PLY56_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ19946.1"
                     /translation="MRTSLRGGVQGGPHNSFVRRVLVVDDNRDAAETLAGLLKLWGHE
                     ATVAHDGPSAIDMARRYRPDTILLDIGLPGMDGYEVARRLRAEGSAKRAFLVAITGYG
                     GDSVLQQSEEAGFDCHLIKPVDPDTLRDLLARVNCD"
     gene            complement(3442..4284)
                     /locus_tag="PLY56_00280"
     CDS             complement(3442..4284)
                     /locus_tag="PLY56_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOJ19947.1"
                     /translation="MQHEGVVTLEDLLDQIHRHRGINLRSCGREVVERRIHGRMRQMR
                     IESYESYLRLLEGSSDEVDYLLSRITLQASAFFRNPAVFSLLRERVIPKLAAVDRQVR
                     VWSAGCGYGEEAYTLAMLFAEAEPWGERAPPEIYGTDIDARALEMAEAGRYSKEATAP
                     LSAAQLTTYFSPEPDRQTVVYRVVDRVRAMVRFALHDLTTAREAPGGGLFDLVCCRNV
                     LIFYTGELHDRAQDLVCRSVAPRGYLCLGPTEWVGAPFASEFDVVSRMARLFQRCASG
                     ARSA"
     gene            4605..5204
                     /gene="nadD"
                     /locus_tag="PLY56_00285"
     CDS             4605..5204
                     /gene="nadD"
                     /locus_tag="PLY56_00285"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOJ19948.1"
                     /translation="MPRIAIMGGTFDPVHTGHLIIAEEARTRFQLDEVVFVPAAIPPH
                     KHRADISPAEDRYAMVLLATASNPHFSVSRVEMERSGPSYTLDTVLEFRKRYGSQAAL
                     YWLTGLDAVLELLTWHEPEAIIRHCRFIAATRPGYDPAMLAERLPQPFVAAIDLLTGP
                     GVEISSSDIRRRIQEGVSARYLTPEPVLDYIRKRRLYAG"
     gene            5251..6507
                     /locus_tag="PLY56_00290"
     CDS             5251..6507
                     /locus_tag="PLY56_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ19949.1"
                     /translation="MSAPIPPRRRRRRWGRLVLAVLFLLAIGTGVFAGMCAYQVISGL
                     DGDGSRGGIQTLTETPFDNKQKVFILVLGVDEKNRLLKTDGRRSDTMILLAADLEQKR
                     LAALSLPRDTRVQIPGRSGYDKLNAAHFYGGVPLVRQTVTQMLGIPIDRFVKTDVAGF
                     RAMVDLIGPIEIDVEKRMDYDDNWGNLHIHLKPGRQLLDGEKAMGYVRFRHDSTGDLG
                     RMKRQQKFMRAVVRQLFTMGNIARLPQIIEKGMEYIETDLTARELLVLANLAREVTMS
                     QITTATLPGTPRYIRQVSYYVPDEERTVALIQKLFFSQAQPAYATVEVLNGNGLPGIA
                     REAARQVSKGGFHVSRTGNADNYDYERTEVRADEEHLADARRVASLLGVGETAVVGVP
                     EEPAESREAPRKEPRIVVVLGKDYRP"
     gene            6568..>6875
                     /gene="rsfS"
                     /locus_tag="PLY56_00295"
     CDS             6568..>6875
                     /gene="rsfS"
                     /locus_tag="PLY56_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ19950.1"
                     /translation="MTSEEKARLAATAAQELKANEVVVLDVQGQTQLADFFVICSGTS
                     DVHIRAIADKIIEKLRENGIEKPRREGVQAARWVLLDYEDVVVHIFSAEECEYYNLES
                     "
     assembly_gap    6876..6885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7140..>7383
                     /locus_tag="PLY56_00300"
     CDS             7140..>7383
                     /locus_tag="PLY56_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ19951.1"
                     /translation="MGTDERAERVDRLLTTAHVQRMRDNLEEARRACEEAVALDPECA
                     AAHEMLGDLAAAREELEAAMKHYREAFRLDGKPATEE"
     assembly_gap    7384..7393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7394..7782
                     /locus_tag="PLY56_00305"
     CDS             <7394..7782
                     /locus_tag="PLY56_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19952.1"
                     /translation="LALRIDERKRGELSTGTRSPSGSRKRLNPSLACALSMLFPGLGQ
                     LYNGEYRKAAGFALLWVLLLPNLYAVFSALLSPLSGHGAKPVSGMTWLLALVQIGVVV
                     VALTDASLSAVAINRGKTQGKSGWEI"
     gene            complement(7913..8248)
                     /locus_tag="PLY56_00310"
     CDS             complement(7913..8248)
                     /locus_tag="PLY56_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19953.1"
                     /translation="MDVTRSVNALRMISRGLKVIAWIVGIAFAAAAIKLAFSVSFIPA
                     DLPTSVEAMLPGNAFLAGVLLLVLGAANWLVLLGLAEGINLVIAIEENTRAAAAAKEA
                     AAPANAGVA"
     gene            8725..10296
                     /locus_tag="PLY56_00315"
     CDS             8725..10296
                     /locus_tag="PLY56_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ19954.1"
                     /translation="MKVTKDQIARDLRALGLENGDVVLMHSALSALGEVEGGADAVID
                     AILEVIGKEGTLAMPTMSSGVFRRESTPSNVGRITEVFRQRPGVLRSLHPTHSVAAIG
                     PQASFLLKDHELSPTAVGPETPFGRLVELRGKVLLLGVDQDRNTLLHGAEEAVNAPYL
                     SEHYATYIDDDGKEVTLRLERYPGPHRDFIGLEPRFRRAGWLRVGKVGRATARLMPAR
                     EMFEETVAALREDPAAVLCDNPACDDCQMQRGKIKAARLQQEEFTLAAVLDDPAAKLA
                     DVARSLRAQGIRHVEIGDTLGDYLLRRRLTEIQAFGDNLRDEGLVVSAFGAGLGRVTP
                     GEDQEFHYRRFEKSLEVLPRLGTQRLRMAALLAGDDRQSATPGVVALLQAAARAASER
                     NAVLVVENEPGTFCDTARHTEEILTAAGSPAVRVAFNPAHFAAVGDKPFLRVYYKGRI
                     KRWMDQLYICDGLWDGTPTLPGRGNGEVKELISILRCRGFRGFFTLRPTTRGRFDPER
                     LREEAERFWFLLDHC"
     gene            10331..11425
                     /gene="rodA"
                     /locus_tag="PLY56_00320"
     CDS             10331..11425
                     /gene="rodA"
                     /locus_tag="PLY56_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOJ19955.1"
                     /translation="MIRRERRVDLWLPACTLGLSGLGLVAIYSATRVDGSDHCWRQAL
                     WLLLGTGFAAIVSRVDYRSMVRLSRLLYVVNLGLLAAVLLTGSSAMGAQRWIALPGGF
                     KLQPSEIAKLVVIITLSTYCTSHRHSLRDFWGLCKSFLFILPPMALIMKQPDLGTSLV
                     LIAVWFGILWVAGARALHLAIIALSGVVAFVGLWHVGLIKDYQKQRLISFLDPSADPR
                     GSGWHTIQSKIAIGSGGLTGKGLLHGSQSQGGFIPEQHTDFINTVIGEELGFIGSSFL
                     LLLFFCLIYRGLRVAWDCADPNGRLIAVGIVTMFTFHVFVNIGMTVHIMPVTGVPLPF
                     LSYGGSSMILNLACIAVLINIERHREDLRF"
     gene            11437..12732
                     /locus_tag="PLY56_00325"
     CDS             11437..12732
                     /locus_tag="PLY56_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ19956.1"
                     /translation="MLQTRPTQGDTSWFVRDRFGLFIHWGIYALPARHEWIKQREQIP
                     DEVYDVYFRHFDPDLYDPNAWAQAAADAGMKYFVVTTKHHDGFCLWDSALTDYKAPNT
                     PYGKDVLRPMVDAFRARGLRVGFYHSLIDWHHPEFVIDDLHSMRNHPDRAKLNEKRDQ
                     SKYVAYLHGQVRELLTQFGQIDIMWFDFSYPAGGNRPETWVGKGRDDWQSEKLLKMVR
                     ELQPHIILNDRLDLPEGWDMKTPEQYQPREWLRVEGQPVVWEACQTFSGSWGYHRDEA
                     SWKSVDQLVQMLVDTVSKGGNLLLNVGPTGRGEFDERALSRLAGIGEWMKRHSRSIYG
                     CTQAPDEFKAPQDCRLTYNPEKKRLYVHVFAWPFKHLHLDGFAGKVEYAQLLNDASEV
                     KMTVPPHQTPGAEGRNTLTLELPVQKPNVTVPVIELFLK"
     gene            12972..>13643
                     /locus_tag="PLY56_00330"
     CDS             12972..>13643
                     /locus_tag="PLY56_00330"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ19957.1"
                     /translation="MARSRLYLFCLALLATAIPGRHLAAADAPELVVIEDFETGTSRW
                     YAVQGEIPEGVKAFVTLTPTVEAKRGEGAGRLRFAAGPNTWGHVQMGIVPAEWTAIGP
                     DRVRLWVKGDGSGEALNLMFGNYERKPPLSFRYRIPLDFTGWRQVTIPLRGFVPEGLP
                     GALREMVLIQLNASGTTRPINILVDEIELLPAERTEPAGTRQDLDLLARGGWDTPAPE
                     GAVPVD"
BASE COUNT         2363 a         4598 c         4318 g         2344 t
ORIGIN      
        1 caagctgccg gccgccgttg ccgagggcga ggggattcgc gacgccttgc gcgagcgcct
       61 gggcatcatc ccggaagtcc agagcaacga cgatggcatc ctcctgcgct tccccgatgc
      121 ggacgccgat ttcccggtgg acgcggtggt gcgcatgtcg cccgcggagg cgcgcgagcg
      181 gctattgcgc gagatccccc actccgccgt ctttggggcg cggttcaggc agaacgccgc
      241 ccgggcgctg ctcctgccgg gggcccggcc gggcaagcgc acccccttct ggctgcagcg
      301 cctgcgcgcc cgagagctcc tggaggccgt cctgcgcttc ccggagttcc ccatcctgat
      361 cgagaccttc cgcgactgcc tggaggacgt gatggacctc ccccacttgg aggaggtgct
      421 ccggggcatc caggaaggcc ggatctcggt ggtgagctac tcggcccttg cgccctcgcc
      481 gatcgcccag ggcctgctcg cccagttcat aagcattttc atgtatgagg gagacgcgcc
      541 ccgggcggaa gcacagctca accaactcgc cgtgaaccgc gctctgctga gcgacgtgct
      601 gaaggacgtg gccctcgacc agttcctgcg ccccgaagcg atcgatacca tggtgcgcca
      661 gctccagcgg atcgagccgg gttaccgcgc gcgcaccgcc gaggagctgg cggttctcct
      721 agagcacctg ggcgatctga cccccgtcga gatagaagcg cgctcccact ccgaccctgg
      781 cccgtgggtg aaagcgcttg ccgccagcgg gcgaatcgtc tcgctggaag tgccaaccgc
      841 cgccgggagc gaaacgcggt gggtgtgcgc ggagtacgcg agcgagtacg cgcgcgcgtt
      901 cgcgctgccc gcgtcccccc cggcggacga ggaggcggtg aacgaggcgc gccgagcgat
      961 tctcacccgc gccctagaga gctggggccc tgtcaccctg gaggcgatcc ggacgcgcta
     1021 cgccttccca tctgaatggc tggccgaggc actggaacag ctggcgcacg agggcaacct
     1081 cgtccgcggc cggttcacgc gcgccgctcc ccctggcgag cagttcatcg cgcgccgcaa
     1141 cctggaggag gtccaccgcc ggacgctttc tctcctgcgc cgcgaagttc gcccggtccc
     1201 gcttcccgtt tacgcggagt tcctggcgcg ctggcaacac gcgcacccgg ccacccggct
     1261 ccgagggcgc gaggggctgc tccaggtgct ccagcaactc cgtggcctgc ctctggcggc
     1321 tcagatctgg gagcgcgacg tcctccccgc gcgcgtggac ggcttctcgc cacgggatct
     1381 ggaggcactg ttcgaagagg gagaggtcgt ctgggtggca tcggcccagg cggatccccg
     1441 gcgcgcgcgc gtcgccttct tcgcccgcgg tgaaggccgc gtctgcctcc cctccatcga
     1501 ggagagtgaa gagcttggga cggaagcccg tgcgatctac gacttcctga agtccgaggg
     1561 aactgcggtc gcggcagaga tccagtcgct cctggggctc acggcacagt cggtcgaacg
     1621 cgggctcacc gagctggcca cccgcgggct ggtcacctgc gattccctcg ccgcgctccg
     1681 ttatctcctg gaggcgccgc aggggagcat caccgccgca gcgccggaga ggcggtggca
     1741 gagctcactc gagtctcaac tccctcgccg aaccctgggc gctacttcct ggcgctcgcg
     1801 ccggccctcg cgcgccgact accaggcagc gaaacggcgc gtcgccgaga ggctgcgtca
     1861 ggcggagcct ctcgccctcg ccgggcgctg gggcctgatc aaccggttga gcatcctcgg
     1921 gcgcgacctc tcgccggacg agcgcgcggc gcgacaggca cgcctgctcc tgcagcggtg
     1981 gggcgtcctt agccgggcca tcgtggatgc ggaggaaggc ccctgggagt gggaggggat
     2041 cgccgcgcac ctgcgcctga tggagatgcg aggcgaggtg cgtcgcgggc ttttcgtgga
     2101 cgggatgcag ggagttcaat acgccctacc ggacgcggtc gagcgcctgc gcgagtgccg
     2161 tgacggcgct cccgaaccgg actccctggt ggtgttgaac gcatgcgacc cagcgcttgc
     2221 ctatggccgg gcagcgcaag aggagcccat ctcgccccat cgcgatcctc tggcgttccc
     2281 gcgaatcccg acaacctggg tggtgcaacg gcgggggcac ccgctcctgg tcgcggaagc
     2341 ggggggcaag gagatcaccc tccttcaggg agcggacgag gaggacgcgc gtgcagccct
     2401 catcatcctg gcggagcacc tgctgcgctc cgcacgtcgg atcacggtgc acacctggaa
     2461 cggcgaaccg gtgctagaga gcgatggtaa gcctctattg gaagcagccg ggttctacca
     2521 cgattaccgt gccatggtcc gcgagtcctc gatagggcga tgaagcgcgt cagtcacaat
     2581 tgacgcgcgc aagaagatct cgcaaggtgt ctggatccac gggtttgatc aggtgacaat
     2641 cgaagccagc ctcctcggat tgctggagga cggaatctcc gccgtagcct gtaatggcaa
     2701 ccagaaaggc cctcttcgca ctcccctcag cacgcaggcg ccgggccact tcatagccat
     2761 ccatgcccgg aaggccgata tcgaggagaa tggtatcggg ccggtagcgc cgggccatat
     2821 caatcgccga cggcccatca tgggcgaccg tagcctcatg accccacagc ttcagcaggc
     2881 cagcgagtgt ctctgcagca tcgcggttgt catccaccac caggacacgt cgcacgaagc
     2941 tgttgtgagg gccgccttgc actccaccac gcaacgaggt tcgcatcggg tccacctctc
     3001 cctgcggggt atccaccggc aggcgaatgc caccaatcct gtcctggccg gggccaccat
     3061 tactgctcta tgcctcggct ctcacagcac tccattcttc gctacgccac cgccgcgtct
     3121 atgctcgaca cgcaaagcag agccgtgcgc gccctcataa aacgagacgg aaaggcagca
     3181 gcatctcgct gcgcggctcc actcgggctc ccgcgcatgg ggcacgttcg tcccacgcgc
     3241 acgtatgcca cgatggagat gcccccggtc gggggtatgt ctctcttcgt cagacggttc
     3301 ctgaacacct cctcataaat tcaccaaacc ctcacggatt caccaaacaa cgtagaaagc
     3361 tcaattccat atccaatccg catcaggatc agggccccgg gccccgagct gtgcaagacc
     3421 gtccgccccc tatggcttca cctacgcaga acgcgcccca ctggcgcacc gctggaagag
     3481 ccgggccatc cggctcacca cgtcgaactc cgaagcgaag ggagccccca cccactcggt
     3541 tgggcccaaa cagagatagc cccttggcgc cacgctgcgg caaaccagat cctgtgctcg
     3601 atcgtgtagc tcacccgtat agaagatcaa gacgttgcgg cagcaaacca aatcgaaaag
     3661 ccctcctccc ggtgcctctc gagcagttgt caggtcatgg agagcaaacc ggaccatcgc
     3721 ccggactcgg tccaccaccc ggtagaccac cgtctggcga tccggctccg gagagaagta
     3781 ggtcgtgagc tgagcggcgc tgagcggcgc ggtcgcctcc ttggagtagc gcccggcctc
     3841 cgccatctcc agagccctgg catcaatatc ggtaccatag atctctgggg gcgcccgctc
     3901 cccccatggc tccgcctccg cgaagagcat cgccagggtg taggcctctt cgccataccc
     3961 acatccggcg ctccagacac gaacctgcct atccacggcg gccaacttgg gaatcacccg
     4021 ctcccgaagc aacgagaaca ccgcggggtt gcggaaaaaa gcactcgcct ggagggtaat
     4081 ccgcgatagg aggtaatcca cctcatcgct gctcccctcg agcaagcgca ggtaactctc
     4141 gtaactctcg atcctcatct gccgcatgcg cccgtgtatc cgacgctcaa ccacctcgcg
     4201 cccgcatgac cggagattga tgccccgatg ccggtggatc tggtccagca gatcctctag
     4261 agtgaccacg ccctcatgtt gcattattcc ctcacgcttg cctgcactca ggctgctgtc
     4321 tagaatacac cagggctcgg tactctccct gcccctgctc ttcacgcatt ccctgacagg
     4381 catgccccca ggacagtctc ttccggggcc aagttccctc ggatgaagga cacagacgca
     4441 gatggcggag tgttctcacc cccaacggcc cctatagacg cggggggatt ccgtccgcgc
     4501 gatagacgcg gcgccggtgt ctacgcccgg gcagttttat tgacaacccc ctggcgggcc
     4561 ggtataatag acttacgtga tgccgcggag ggcttgcgct tatcgtgccg agaatcgcga
     4621 tcatgggggg cactttcgat ccggtgcaca cagggcacct gatcatcgct gaggaggcgc
     4681 gcacccgctt ccagctggat gaggtcgtct ttgtaccagc cgcgattccg ccacacaagc
     4741 atcgtgccga tatctctcct gccgaggatc gctatgcgat ggtgctcctc gccaccgcga
     4801 gcaatccgca cttctctgta tcgcgcgtcg agatggagcg ctctggccca tcctacacgc
     4861 tcgatacggt actcgagttc cgcaagcgct atggttccca ggccgcgctc tactggctga
     4921 ccgggctgga tgccgtcctg gagctcctca cctggcatga gccggaggcg atcatccgcc
     4981 actgtcgctt catcgcggcg acgcgccccg gatatgaccc ggcgatgcta gcagagcgcc
     5041 tgccccaacc attcgttgcc gcgattgatc tgttgacggg tcccggcgta gagatctcgt
     5101 cgagcgatat ccgccgccgt attcaggagg gcgtgtcggc gcgctatctc acgcccgagc
     5161 ctgtcctgga ctatatccgg aagagacgct tgtatgcagg atgacctgcc gcccagaaga
     5221 ataccgcccc gacaacccgt gaggccggcc gtgtccgctc ccatcccgcc acgccggcgg
     5281 cgccggcggt ggggccgcct ggtgctggcc gtcctctttc tcctggcgat tggcacgggc
     5341 gtcttcgccg gcatgtgtgc ctatcaggtg atctcaggcc tggacggcga tggctccagg
     5401 ggaggcatcc agacacttac ggagacgcct ttcgacaata agcagaaggt cttcatcctg
     5461 gtgctcggcg tggatgaaaa gaaccggctg cttaagacag acgggcgccg gtccgatacg
     5521 atgatcctgc tcgcggccga tctggagcag aagcgcctcg ccgcgctgtc gctcccgcgc
     5581 gatacccggg tgcaaatccc cggtcgcagc ggctatgaca agctaaacgc cgcgcacttc
     5641 tatgggggcg ttccgctggt gcgtcagacg gtcacccaga tgctcggaat cccgattgac
     5701 cgcttcgtga agacggacgt ggccggcttt cgggcgatgg tcgatctcat cggtccgatt
     5761 gagatcgacg tcgagaagcg catggattac gacgacaact gggggaatct gcacatccac
     5821 ctcaagcccg gccggcaact gctcgatggc gagaaggcga tgggttatgt gcgcttccgc
     5881 cacgattcga ccggcgacct ggggcggatg aagcgccagc agaagttcat gcgcgccgtc
     5941 gtgcgccagc tcttcaccat gggcaacatc gcgcgcctcc cgcagatcat tgaaaagggg
     6001 atggagtata tcgagacgga tctcaccgcg cgcgaactcc tggtgctcgc caacctggcg
     6061 cgcgaagtca ccatgtcgca gatcaccacg gcgacgttgc cgggaacacc gcgctacatc
     6121 cgtcaggtca gctactatgt gccggatgag gagcgcacgg tggccctcat ccaaaagctc
     6181 ttcttcagcc aagcgcagcc cgcatatgcc accgtggagg tactcaatgg caatgggctc
     6241 cctggcatcg cgcgtgaagc cgctcgtcag gtgagcaaag gagggttcca cgtttcgcgg
     6301 accggtaacg cggacaacta tgactacgag aggaccgaag tgcgagctga cgaggagcac
     6361 ctggcggatg cccgtcgcgt ggcctctctc ctcggtgtcg gagagaccgc cgtggtgggt
     6421 gtgccggaag agccggccga atcgcgtgag gccccccgca aggagccgcg catcgtcgtc
     6481 gtgctcggca aggattaccg gccataatgt gtccgcggcc tgcggccccc gtcgcctggg
     6541 gcgcgcgcgg ccctggaggg ttccgatttg acatctgaag agaaagcaag gctggcagcc
     6601 acagccgctc aagagctcaa agccaacgaa gtcgtcgttc tggacgtgca gggccagacg
     6661 cagctcgccg atttcttcgt catctgcagc ggcaccagcg acgttcacat ccgggcgatc
     6721 gccgacaaga ttatcgagaa gctccgggag aatggcatcg agaagccgcg gcgcgaggga
     6781 gtgcaagcgg cgcgctgggt gctcctcgat tacgaggatg tggtggtgca catcttctcc
     6841 gccgaggagt gcgagtacta caacctggag tcgttnnnnn nnnnncggcc agcacgagcc
     6901 cgccatcgcc tgagcgccca agaaggcgcg cgcgtcgcag cgcaacacgg gtgtagtgcg
     6961 gttctgatta cgcgttcggt cgggcggctc gcgttgtggc ccggcttcca ttcgcgggcc
     7021 atttcgcgca ggcggggcac cgcgtatcgc cggcccgccg aacgccctcg cacatgcact
     7081 cgcgttctct gatcccacct cgtagcagga tctcgtcgtg aagcaaggaa tagcagctta
     7141 tgggcactga tgagagagcg gagcgagtgg atcggttgtt gaccacggct cacgtgcagc
     7201 gcatgcgaga caacctggag gaggcgcgcc gtgcctgcga ggaggcggtt gccctcgatc
     7261 cggagtgcgc cgcggctcac gagatgttgg gggatctcgc ggcggcgcgc gaagagctcg
     7321 aagccgcgat gaagcactac cgtgaagcgt ttcgcctcga tggcaagccg gctactgagg
     7381 agannnnnnn nnntcctcgc cctgcggatc gatgagagga agcgcgggga gctgagcacc
     7441 ggaacgcgct ctcccagtgg ctcgcggaag cgcttgaatc cttcgctcgc ctgcgcgttg
     7501 agcatgctct tccccggcct ggggcaactc tacaacggcg agtaccggaa ggcggctggc
     7561 ttcgcgcttc tctgggtgct cctccttccg aacctctacg cggtcttttc cgcgctcctc
     7621 agcccgctgt cgggccacgg cgccaaaccg gtctctggga tgacgtggtt gctcgcgctg
     7681 gtgcagatcg gagtcgtagt cgtcgccttg accgatgcct cgctctccgc ggtggccatc
     7741 aaccgcggaa agacacaggg caagagcggc tgggagatct gacgaggacg agcggagacg
     7801 ggcgatacgt gctcccgccc ggcctctcca cgcccaatcc cccgctgagc agcctgcacg
     7861 ccggggatcg ctggaagaca ccgggcggaa gcggaaagag tcagcagcgt ttctaggcaa
     7921 cgccggcatt cgcgggcgct gcggcttctt tcgcggctgc ggcagcgcgc gtgttctcct
     7981 cgatggcgat cacgaggttg atcccctcgg ccagcccgag cagaacgagc cagttcgcgg
     8041 cacccagcac caggagcaac acgcccgcca agaacgcgtt ccctggcagc atggcctcga
     8101 cactcgtcgg caggtccgcc gggatgaacg agaccgagaa ggccagcttg atcgccgcgg
     8161 cggcgaaggc gatcccgacg atccaggcga tcaccttcag gcccctggag atcatccgca
     8221 acgcattcac agaacgagtc acgtccattc gaatcctctc tttcctaaca gagccctgtc
     8281 ccctatggca tcagggcggg atgcagactg cgaagcggca gactgcgtcc aggcccggcg
     8341 atccaggaga gacggcggtg cgattctcgc cggacgcagc ggaccggcgc taccgtagtg
     8401 gcgccgctcc atcctcgatg tttggcaagc gttcttctcg cgctccagtt cgccgaaaaa
     8461 ccggccactc cttcaaccgc tggagaaggc cgctctcggc ggtacgccac gcttgagacg
     8521 ttccttcgcc agcttgcccg gtgttccttc ccgtatcagc tggctactta cccggtgata
     8581 agcaggcgtg cgtgggtaac aggtatggag gtgcgacgtt tttgcctcac atacgcgggt
     8641 ggtcgcgtag attcctgctg caggatgtgg tgttctctcc accgaaaaga gagaaaccac
     8701 actcgctcat cggggggctt catcatgaag gtgaccaaag accaaatcgc ccgcgatctg
     8761 cgcgcgctcg gattggaaaa cggcgatgtt gtcttgatgc atagcgcgct ctccgcgctt
     8821 ggcgaagtcg aaggcggcgc cgacgccgtg atcgacgcga tcctcgaggt gatcgggaag
     8881 gagggcacgc tcgctatgcc cacgatgtcc tccggcgtct tccgccgcga gagcacgccc
     8941 tccaacgtcg gccggatcac cgaagtcttc cggcagcgcc caggggtgtt gcgcagcctg
     9001 caccccacgc actccgtggc cgccatcggg ccgcaagcct ccttcctcct caaggatcat
     9061 gaactctcgc ccacggccgt gggccccgag acacccttcg gacgcctggt ggaactgcgt
     9121 ggcaaggtgc ttctgctggg cgttgaccaa gaccgcaaca ccctgctcca cggcgccgag
     9181 gaggcggtga acgcgcccta cctctcggaa cattacgcca cctacatcga tgacgacggc
     9241 aaggaggtga ccctccgcct ggaacgctac cccgggccgc accgcgactt tatcggcctc
     9301 gagccccgct tccgccgggc aggctggctg cgggttggca aggtgggccg ggccacggca
     9361 cggttgatgc ccgcacgcga gatgttcgaa gagacggtcg ccgccttgcg cgaggacccc
     9421 gcagcggtcc tctgcgacaa ccccgcgtgc gatgattgcc agatgcagcg aggtaagatc
     9481 aaggcggccc gcctacaaca agaggagttc acgctcgccg ccgtcctgga tgacccggca
     9541 gccaagctcg cggacgttgc gcggagcctg cgcgcccagg ggatccgaca cgtggagatc
     9601 ggcgataccc tgggcgacta cctcttgcga cgtcggctca ccgagatcca agcctttggt
     9661 gacaatctgc gcgatgaggg gctggtggtc tcagcatttg gcgccggcct gggccgcgtg
     9721 actcccggtg aagaccagga gttccattac cggcgctttg aaaagagttt ggaggtgctc
     9781 ccccgcctcg gaacgcagcg cctgcgcatg gccgccctcc tggctggcga cgaccggcaa
     9841 tcggccacgc ccggggtggt ggcgctgttg caggccgccg ctcgcgcggc gagcgagcgc
     9901 aacgcggttc tggtggtgga gaacgagccg ggcacgttct gcgacacagc gcgccatacc
     9961 gaggagatcc tgacggctgc gggctctccc gccgtgcgcg tggccttcaa ccccgcccat
    10021 ttcgcggccg tgggtgacaa gccgttcctg cgcgtctact acaagggccg catcaagcgc
    10081 tggatggacc aactctacat ctgcgacggc ctgtgggacg gcacgcccac gctccctgga
    10141 cgcggaaatg gcgaggtgaa ggaactgatc tccatcctgc gctgtcgtgg ttttaggggc
    10201 ttcttcacgc tccgcccgac gacgcgaggg cgcttcgatc cggaacgcct gcgggaagaa
    10261 gcagagcgct tttggttctt gctagaccat tgttgatgag ctgctccagg ggaagtcggt
    10321 taagttgggg atgatacggc gtgagcggcg cgtggatctg tggcttcctg catgcacact
    10381 cggcctctcc gggctggggc tcgtcgccat ctatagcgcc acgcgcgtgg atggcagcga
    10441 ccactgctgg aggcaggccc tgtggttgct cctgggcacc ggtttcgcgg ccatcgtctc
    10501 ccgggtggac taccggagca tggtgcgcct cagtcgattg ctctacgtcg tgaatctggg
    10561 gttgctggca gccgtgctgc tcaccggctc ttcggccatg ggcgcccaac gctggatcgc
    10621 cctgcctggc gggttcaagc tccagccctc cgagattgcc aagctcgtgg tgatcatcac
    10681 gctctccacc tactgcacga gccaccggca ctcgctacgc gacttctggg ggctttgcaa
    10741 gagcttcctc ttcatcctgc ccccgatggc gctgatcatg aagcagccgg acctgggcac
    10801 gtcgctcgtt ctgatcgccg tgtggttcgg gattctctgg gtggcgggcg cgcgcgcgct
    10861 gcacctggcg atcatcgccc tctctggcgt ggtcgccttt gtcggcctgt ggcacgtcgg
    10921 gctcatcaaa gactaccaga agcagcggct gatctcgttc ctggacccct ccgccgatcc
    10981 acgcggctcc gggtggcata cgatccagtc gaagatcgcc atcggatccg gggggctgac
    11041 cggcaagggc ctgctgcatg gctcgcagag ccagggtggc ttcatcccag agcagcatac
    11101 ggacttcatc aacaccgtga tcggcgagga gttgggcttc atcggctctt cgtttctcct
    11161 gctcctcttc ttctgcctga tctaccgtgg attgcgggtg gcgtgggatt gcgccgatcc
    11221 gaatggccgc ctgattgccg ttgggatcgt cacgatgttc accttccacg tcttcgtgaa
    11281 cattggcatg actgtccata tcatgcctgt caccggcgtg ccgctcccct tcctctccta
    11341 tggggggagc agcatgatcc tgaacctcgc gtgcatcgcg gtcctgatca acatcgagcg
    11401 gcaccgtgag gatctgcgtt tctgaggagg caccacatgc tacagacaag accgacccag
    11461 ggtgatacga gttggtttgt cagagatcgc ttcggtctat tcatccattg gggcatctat
    11521 gccctccccg cgcgccacga gtggatcaag cagcgcgagc agatcccgga tgaggtctac
    11581 gacgtctact tccgccactt cgaccccgac ctgtacgatc cgaatgcctg ggcgcaagcc
    11641 gcggcggatg ccggcatgaa gtacttcgtc gtcaccacca agcaccatga cggcttctgc
    11701 ctctgggatt cggcgctcac cgactacaag gcgccgaaca cgccctatgg gaaagacgtc
    11761 cttcgcccga tggtggacgc gttccgcgcc cggggcctgc gcgtcggctt ctaccactcg
    11821 ctcatcgact ggcaccatcc ggagttcgtg atcgacgacc tccattcgat gcgcaaccac
    11881 cccgaccgcg ccaagctgaa cgagaagcgc gatcaatcga agtacgtcgc ctacctccat
    11941 ggccaggtgc gcgagctcct cacccagttt ggccagatcg acatcatgtg gttcgatttc
    12001 tcctatcccg ccggcggcaa ccgtccggag acgtgggtgg gcaagggccg cgatgactgg
    12061 cagagcgaga agctgctgaa gatggtccgg gagctgcaac cccatatcat cctcaatgac
    12121 cgcctcgatc tccccgaagg ctgggatatg aagacgcccg agcagtatca gccgcgcgag
    12181 tggctgcggg tggagggcca gccggtggtc tgggaggcgt gccagacctt ctccggctcc
    12241 tggggctacc accgcgacga ggcctcctgg aagagcgtgg atcagctcgt ccagatgctc
    12301 gtcgataccg tcagcaaggg cggcaacctg ctgctcaacg tcggccccac cgggcgcggc
    12361 gagttcgacg agcgcgcgct ctcgcgcctg gccggcatcg gcgagtggat gaagcggcac
    12421 agccgctcga tctacgggtg cacgcaggcg ccggacgagt tcaaggcgcc ccaggattgc
    12481 cggctcacct acaacccgga aaagaagcgg ctctacgtcc acgtcttcgc ttggccgttc
    12541 aagcacctcc acctcgacgg cttcgccggc aaggtggagt acgcccagct cctcaacgac
    12601 gcctccgagg tgaagatgac cgtcccgccg caccagacac cgggcgccga gggccggaac
    12661 acgctgacgc tggagctccc ggtacagaag ccgaacgtca ctgtgccggt gatcgagctg
    12721 ttcctgaagt agggggctgc ccacttccat gcgcggcgcg ggaccatagg tcccgcgccg
    12781 cgcgcattct ccgcgccttc tcccaccggg aggatcctcg gcgaagccca tcgaaacacc
    12841 atcggaaaac ggacccccat cgaccgcgag gggaagccca acgctatcgg cgaggtgata
    12901 ccctggcaac acctcgcccc gcgggcgcaa ggtgcgtttc gccaggtaga ccgccgctcc
    12961 gggagggact catggcacgc tcgcgcttgt atctcttctg cctggcgctg ctggcaaccg
    13021 ccatcccggg caggcacctg gcggcagccg atgcgccgga actggtggtg atcgaggatt
    13081 tcgagaccgg cacgagccgc tggtacgccg ttcagggcga aatcccggaa ggcgtgaagg
    13141 cgttcgtcac gctcacgcct accgtggaag caaaacgggg cgagggcgcc ggccggctcc
    13201 gcttcgctgc cgggcccaac acctgggggc acgtgcagat gggcatcgtt cccgccgaat
    13261 ggacggccat cggcccggac cgggttcgcc tctgggtgaa gggcgatggc tccggcgagg
    13321 cgctcaacct gatgttcggg aactacgagc gcaagccacc cctctccttt cgctaccgga
    13381 tccctctcga cttcaccggc tggcgccagg tgacgatccc cttgcgcggc ttcgttcccg
    13441 agggcctgcc aggcgccctg cgcgagatgg tcctcatcca gctcaacgcc tccggcacca
    13501 cgcgccccat caacatcctc gtcgacgaga tcgagcttct gcccgccgag agaaccgaac
    13561 ccgccggcac ccgccaggat ctcgacctgc tggcccgcgg cggatgggac actcccgcgc
    13621 ccgagggcgc cgtgcccgta gac
//
LOCUS       DAOSCV010000006        13580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3072_length_13580_cov_3.382181, whole genome shotgun sequence.
ACCESSION   DAOSCV010000006 DAOSCV010000000
VERSION     DAOSCV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3072_length_13580_cov_3.382181"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..150
                     /gene="sucC"
                     /locus_tag="PLY56_00335"
                     /pseudo
     CDS             1..150
                     /gene="sucC"
                     /locus_tag="PLY56_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386312.1"
                     /note="catalyzes the interconversion of succinyl-CoA and
                     succinate; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit beta"
     gene            507..986
                     /locus_tag="PLY56_00340"
     CDS             507..986
                     /locus_tag="PLY56_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ19958.1"
                     /translation="MQFKNLKASELDGRVELPGLNEWIESLNGTEAALLIAAPAGTGK
                     TAAVGAIARKLERDVMQCDLLQVFEYPDSREAFRDLLTIIENLRSIVFQAEGIGRLGD
                     RFQEAGDASVLDLMRDWLADKKERLRSRGVVLVATARSLNGLPEDLLAQFDKVFSAP"
     gene            1358..1720
                     /locus_tag="PLY56_00345"
     CDS             1358..1720
                     /locus_tag="PLY56_00345"
                     /inference="COORDINATES: protein motif:HMM:NF036463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5335 family protein"
                     /protein_id="HOJ19959.1"
                     /translation="MAREIPRSEWTEFLTDFTVSNEGRIARVEVDGRCLGDAEGEDAF
                     VLTGVEAGVQAEDEDTIVVALADIEGEQQGRVTHRIEDVTTLRVREHDGMVDGLEIET
                     REGARAVVHLGEEMPATA"
     gene            complement(1934..2581)
                     /locus_tag="PLY56_00350"
     CDS             complement(1934..2581)
                     /locus_tag="PLY56_00350"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ19960.1"
                     /translation="MPENRLLVDKEHLPVYYEATQLLTQNHVPHLLGGGLMVSLYGRG
                     RDTKDIDFYIHPRDKNRAMSVLNAAGFYTEETEKAWLLKAEKAGAPVDLIVHSSGVAD
                     LDEDALQHARTIMLGGYPFRGFGPEDMLLRKIYSWQEGRPDWWDAVSIVAGVGPEMDW
                     PYFVRRVPAHNPGRALSFLLFSHAHFAGEQVPWSAIAELGTPFFCEGVPLPRGRE"
     gene            2745..3413
                     /locus_tag="PLY56_00355"
     CDS             2745..3413
                     /locus_tag="PLY56_00355"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOJ19961.1"
                     /translation="MELPILPLHTVLFPQMVLRLLVTEPPHLEMVQRCMDGDRTLGIV
                     LARPGKTLDDIELCRVGTSAYIADLTEQGDGRTLLLVFGQRRFRLLDYRDGEYFVTGN
                     VEWLPETGEDASVGVSVVTELLQCYLADILKPLGLAHPFPGEVEIPQEATELSYVVAM
                     LLPLALSEKQRLLELPGPAARLEAEVPLLRQQIEADRQGGRWRSIARPLGKQDAEWMA
                     QWRN"
     gene            complement(3424..4860)
                     /locus_tag="PLY56_00360"
     CDS             complement(3424..4860)
                     /locus_tag="PLY56_00360"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ19962.1"
                     /translation="MERISGDWQFLFAQDGGYVRLPIWGHIPLSRAARRVIEHPDFVR
                     LQQVKQLLFVHYAFPGATHTRLEHCIGTYHLARQLLLSLLANPYNNDPETGEPRIAPD
                     EGRVFLAAALLHDIGHYPCAHLLDHLPLRDSAGQPLFLDHEERAREYLDPERPGGLAT
                     VLREEWGISDPRSVANTIAPVDASGEDNLPARLLSGIVDPDKMDYLMRDADACGVPYG
                     HIDTDRLIESLVLDVGPGGRRIAITEKGVAPLESLIFSKYMMFRHIYWHHTARIASAM
                     FGRLLQDGVAAGVIRPEWFYSLSDDALLCHLASPAVAGAFPAGELAEMLQRRELYKRA
                     VTLYPEPGVGEPEFRLGQEQIAHLVQLHRDPTRRRAVEVAVCELLARRTGIPLRGHEV
                     LLDIPEPESVFDVEDFNTLRVVVSPQRDRSKRYLVPFNDYGFSQMKSEFAQSFERYSR
                     KVRVVCRPDLRTAVLDAWESAWKVILAA"
     gene            complement(5263..8655)
                     /locus_tag="PLY56_00365"
     CDS             complement(5263..8655)
                     /locus_tag="PLY56_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19963.1"
                     /translation="MALSHSYLYLIPLLLVIATGAWGAAADLPDPYRRSDQPTIGPAP
                     VPVMEQVFWVEAPFDPGLEVKITLPEGVQLLDRTKPGRGRTRTRFYLRANRGIEKGSI
                     TLAPANGPTFSLPLTVRTYRQDIEEQIRVVPGIDPTARKRGRSYYTDRKVALARQNLE
                     RYPKLREELVRPSRYDGKSDEEIFASLPSWNVPRECYSNWPCPKCGEAIYRVNAFYPW
                     RNNTATPFKAQCPVCKQLFPTNDYARGDFTSGDYPDDGWGWDPGTGKRDDYAGWVAYY
                     NHHAVWQNFGGEVLRMAQRALLFGDRAAAHKVGVILARVAYVYPGMNMRWQQVDSHYL
                     RPGRLLLDGNWERNEILVPLVRAYDAVFDLLGEDQALADFLHTKDPAIRTPVDVRALI
                     DTYVVQLFGWDWMRRELSGGNMGAREEDMAQFAVCADMGAVSDRWIEELFTHAYNSGT
                     NMGGFDDETLVNTTTREGPVWISALGYAVDYLNSKSDMAEILSEVNSPRWKARCNLYD
                     ETLYPKLRAEFDTWLDFVVAGRFGPSYGDSGAGNTAAYPKGIPAAVRTTYERAYRRWP
                     TDRLARALFRLGPRDPGLFEEDIWPQVAAHAKKAGPEPPLESRVLDGVGFVFLESRAH
                     AAQEEERAGIALRYGYGRGHHHQDNLNIEMFAKGLSVAPELGYPCWAHPMGDTAHVAH
                     HNTGMIDRSGQYRSAISRGDLELFAGAPEASFADVSAAPDGFPNRVYRRAVCLADAPG
                     GNVYLLDILRLAGGTTRTYCFHGPGHDDFQSSLTFGEKGTVYKVSGITRGLANNMVEP
                     QAAAFDGDVWADWKCQEKPVRLRITHLGRPGRRYITARCAKPDIPPIRYLFAEEEGAD
                     GASEFIALWQPYEGEPFIQKVERLPVEGARPGGEFAPVALRVTLAGGQVDTFFYSWNP
                     DALLRAGGLEFRGSFGYWSEKEGVLRALHLVNGERLRRGNEGVSEIPPAFRSRVIRAD
                     LRKSEITLEHPIPRDLVRPGQLIYLRGGNHRSAYHLEEASDDGKQARLDLNSILFRSK
                     LMSVSPDRRCLITETAPPVEAASGFKPGYYNGTVATGEDLKARYRVVSVEKESILLDR
                     PVKDEDFPDVDGDGRRMVSLYEFGEGDEVVVYRSVFIRKGQP"
     gene            9005..9745
                     /locus_tag="PLY56_00370"
     CDS             9005..9745
                     /locus_tag="PLY56_00370"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HOJ19964.1"
                     /translation="MAEEQRLEWEALPTEELVEELEYVGLRLPLGLVEEILRRGKDAL
                     EPLGRRVADASLWESDGDERWAPLHALHLLGAIGEPAAVGFILDALRINPEPDEIIEN
                     TPTVLGHLGPEVIPELSRFILDESVDGLMRGVACDGLVSIAVLHPEHRDEVARFLRRF
                     LEEAAQHDREAVTAAILSLVELRDKESRPAIAAAFRSGHVDTDLVTLEDVHEAFQQPE
                     RVRDDWHFTGDPLEFFQPERLEKMRSKE"
     gene            9770..10666
                     /gene="xerC"
                     /locus_tag="PLY56_00375"
     CDS             9770..10666
                     /gene="xerC"
                     /locus_tag="PLY56_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOJ19965.1"
                     /translation="MDDLVEQFLGHLASHRRLSQMTVKSYAEDLAHFLRFLESPDVAC
                     EEWGAVDVQVIRRYLGALQDQEYRKTTIARRLSCLRSFFRYLVRRRVITADPMAGLMA
                     PRLERRLPKVANRELLDALLEAPDETTPAGLRDRAILELLYATGVRGSELEAIDVSDV
                     EFGRREIRVLGKGSKERVVIFGKAAQEALAEYLSRGRPHLLKENRDEPALFLNRFGKR
                     LSVRSVRDILEKYLMQVAAELHLSPHSLRHSFATHLLDGGADLRAVQELLGHSSLGTT
                     QVYTHVSNQRLQAAFRRAHPRA"
     gene            10795..11328
                     /gene="hslV"
                     /locus_tag="PLY56_00380"
     CDS             10795..11328
                     /gene="hslV"
                     /locus_tag="PLY56_00380"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009442561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOJ19966.1"
                     /translation="MKVHHTTIVCVRRDGKTAIAGDGQVTFENTIMKQGSRKVQRLAG
                     GKVLAGYAGAAADALTLMERLEAKLDQYAANLERAGFELVKEWRTDRVLRRLEALLVV
                     ADERGMFVISGGGDMIRPDGDVIAVGSGGGYALAAARALMKHTSLSAEEIAREALGIA
                     ASICVYTNENITVELLG"
     gene            11398..12819
                     /gene="hslU"
                     /locus_tag="PLY56_00385"
     CDS             11398..12819
                     /gene="hslU"
                     /locus_tag="PLY56_00385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOJ19967.1"
                     /translation="MRQLTPREIVEELNKYIVGQDKAKRAVAIALRNRYRRRLLPEAL
                     REEVIPKNILMIGPTGVGKTEIARRLARLANAPFVKVEATKFTEVGYVGRDVDSMVRD
                     LTQVAYRMVQQERIEDVREEAERLAIERIVDLLEPLPRSRQEGSASDFLMEAARQFMG
                     ARAGGTTRPAPVQEPSASPNLEARRAELRQKIIAGELDGDRIELEVEETHAPFMQVFS
                     QQGLEEMGMDLQGMLGNIMPRRTKKRNVSIAEAREILQKEEAEKLIDNDEVVRVAIER
                     TEQDGIIFVDELDKIAGREKGAGPDVSREGVQRDILPIIEGATVVTKYGPVRTDHILF
                     IAAGAFHMSKPSDLIPELQGRLPIRVELESLGEEEFVRILSEPENALTKQYAAMLETE
                     GVHVEFTEEGIRTIASIAVQVNSRTENIGARRLHTVMEHLFEELSFLAPDIQPATVRI
                     TEQYVRERLEGIVKNEDLSRYIL"
     gene            complement(13062..>13580)
                     /locus_tag="PLY56_00390"
     CDS             complement(13062..>13580)
                     /locus_tag="PLY56_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ19968.1"
                     /translation="IPPVGNKIDWEAELAVVIGRRGKAIAEADALSYVVGYTVINDVS
                     ERALKIRERSKSEEWDKFFDWLNGKWFDTFAPMGPWIVTADEIPNPQRLQVTLTVNGA
                     EMQNGNTGQMIFPVAELIHYISQFITLEPGDVIATGTPAGVGSGRGIFLKPGDRVRTT
                     IERIGSIENVVE"
BASE COUNT         2392 a         4249 c         4611 g         2328 t
ORIGIN      
        1 ggcggtgcga ccaaggagcg cgtgacggcg gccttcaaga tcatcctgtc cgaccccaac
       61 gtcgaaggca tcctggtcaa catctttggc ggcatcatgc gctgcgacgt gatcgccgaa
      121 ggtgtggtgg ccgccgcgcg ggaagtggcg tggtctcctg ccgatgagaa ggccgccatg
      181 ctccacacgc aggaccagcg ccagctgcat gccgttcgcg gccaggtcgc ggatgccatc
      241 gaggcgctcc tgaagcggct cccggcgcgt tgacatcccc cccagttcat gttatgatgc
      301 cgctagcggt ggaagcatgg attcaccgag ggcttctggt tcgccagagg ctggcgaccc
      361 tgtgcccgcg ccagaaagcc ggtcgagggt gtctgcaaat cggtttatcc aggtgcggac
      421 caggcacgat gggttgctga gccgggacgg atgacaagag cgacgaccag attcgtccac
      481 tgtcaccttt ccataaggag tgatggatgc agtttaagaa cctgaaggcg tccgagctgg
      541 atgggcgggt ggagctgccc ggcctgaatg agtggatcga gagcctgaac ggcacggaag
      601 ccgcgcttct gatcgccgcc cctgcgggca cgggcaagac ggctgccgtg ggcgccattg
      661 cccgcaagct ggagcgcgac gtcatgcagt gcgacctcct gcaggtcttc gagtatcccg
      721 acagccgcga ggcgttccga gacctcctca cgatcatcga aaacctgcgc tcgatcgtgt
      781 ttcaggcgga ggggattggc cggctgggag accgcttcca ggaagctggc gatgcctcgg
      841 tcctggatct gatgcgcgac tggctggctg ataagaaaga gcgtctccgc tcgcgcggcg
      901 tcgtcttggt cgccaccgcc cggagcctga acggcctgcc cgaggatctg ctcgcgcagt
      961 tcgacaaggt cttttctgct ccctgagtct acggggaaac cggatgcgcc agtagtccga
     1021 ggcagcgcgc tctctagcag gccgctgcct cggacgcggc aagccaatac ccgaatggca
     1081 cccccggtgc cgcctccccc gagaaggcct gcgccaccgg gtggcgttct tctgtcgggc
     1141 ctccccagaa acatctctct tcatccccgc gtgacggctg gtcctctgga tcagccggtt
     1201 gtttgcccct ccggatgtaa aatcgctcca aaacggcctg cttcttgctt cgtgcccgct
     1261 cgtagttttg cagcggaaag ccgcggggaa tgatggcggc agcgcgcgcc gccgcggcga
     1321 cgggcgaccg atgtgagcgt aagtgaggag ggtttctatg gccagggaga tcccgcggag
     1381 cgagtggacg gagttcctga cagatttcac cgtgagtaat gagggccgca tagcaagagt
     1441 ggaggtcgat ggccggtgcc tgggagacgc cgagggggaa gatgcctttg ttctcaccgg
     1501 cgtggaggcc ggggtgcagg ccgaggatga ggatacgatt gtcgtggcgc tggcggatat
     1561 cgagggcgag cagcagggcc gcgtcaccca ccgcatcgag gatgtcacca cgctgcgcgt
     1621 tcgcgagcac gatggcatgg tggatggcct cgagatcgag acccgcgagg gggcgcgggc
     1681 tgtcgtccat ctcggcgagg agatgccggc gaccgcgtga gatgcacctc caaagcggca
     1741 gccgcggcgg ctggcatagt tctaccggct gggcacgagg cggacagtgt gaatcgtacc
     1801 gcgaccgccg ggcgggatgg gatgccagag tcggacgagc gcgctgaggg ggcgtagtct
     1861 tgccacgtgt ctgtttgaag cgggtgcgct gccgggaaat ggatgggtga tccggggccg
     1921 gggggatagg cgcctactcc cggcctcggg ggagtggcac gccctcgcag aagaagggcg
     1981 tgcccagctc cgcgatggcc gaccagggca cctgctcgcc cgcgaaatgg gcatgggaga
     2041 agagcagaaa actgagcgcc ctgcccgggt tgtgcgcggg cacccggcgc acgaagtagg
     2101 gccagtccat ctcaggaccc actccggcga cgatggagac cgcgtcccac cagtcgggcc
     2161 ggccctcctg ccaggagtag atcttccgca ggagcatatc ctccggaccg aatccgcgaa
     2221 acgggtagcc accaagcatg atggtgcgcg catgctgaag cgcatcctca tcgagatcgg
     2281 caacgccgct ggagtggacg atcagatcga ccggagcgcc tgccttctcg gccttgagga
     2341 gccacgcctt ctccgtctcc tcggtgtaga agccggccgc gtttaagacc gacatcgccc
     2401 ggttcttgtc acgcgggtgg atgtagaagt cgatatcctt ggtatcgcgc ccccgaccgt
     2461 agaggctgac catcaggccc ccgcccagga ggtggggcac gtggttctgc gtcaggagct
     2521 gtgtcgcctc gtagtagacc ggcaggtgct ccttgtccac gagtagccgg ttctctggca
     2581 tgccctcacc ccccttttcc acgcatcaac gcggatgctc gctgatgcaa ccatacccga
     2641 cactctcctc tcccatgcgc aactacagga ggaccgtcgg gcgggggcga acagaagtga
     2701 cagtcagggc gagacgacac atcagggata ggaggatcaa ggcgatggag ctgccgatat
     2761 tgcccttgca taccgtgctc ttcccccaga tggtcctgcg cctgctggtg acggagccgc
     2821 cccatctcga gatggtgcag cgctgcatgg atggcgacag gacgctgggg atcgtgttgg
     2881 cgcgaccggg aaagacgctg gacgatatcg agctctgccg cgtggggacg agtgcctaca
     2941 tcgccgatct gactgaacag ggagatggac gcacgctcct cctcgtcttc gggcagcgcc
     3001 gtttccgcct gctcgattac cgcgatggcg agtacttcgt cacggggaac gtcgaatggc
     3061 ttccggagac aggggaagac gccagcgtgg gggtgtcggt cgtgacagag cttctgcaat
     3121 gctatctcgc cgatatcctc aagccactcg gcctggccca cccctttccg ggtgaggtcg
     3181 agattccgca agaggccacg gagctctcgt acgtggtcgc catgctcctg ccgctggctc
     3241 tctccgaaaa acaacgcctt ctggagctgc ctggacccgc tgcccggttg gaggcggagg
     3301 tgcccctcct tcgccagcag atcgaggcgg accggcaggg cggccgctgg cggagcatcg
     3361 cccgtccgct gggcaagcaa gacgccgagt ggatggcgca gtggcgaaac tgagcgcccc
     3421 ggatcaggcc gccagaatca ctttccaggc gctctcccag gcgtctagca cagccgtgcg
     3481 caggtccggt cggcagacca cgcgcacctt gcggctgtat cgctcaaatg actgggcaaa
     3541 ctcggatttc atctgcgaga acccatagtc gttgaacggc accaggtagc gcttgcttcg
     3601 atcgcgttgg ggcgagacga cgacgcggag tgtgttgaaa tcctccacgt cgaagacgct
     3661 ctcgggctcg gggatgtcga ggagcacctc gtggccccgc agcggaatgc cggtgcgtcg
     3721 ggcgagaagc tcgcacaccg ctacctccac agcgcgccgc cgcgtgggat cgcggtggag
     3781 ctgcaccaga tgcgcaatct gctcctgccc cagacgaaac tctggctcgc caactcccgg
     3841 ttccgggtaa agggtcaccg cgcgcttgta cagttcgcgc cgctggagca tctcggccag
     3901 ttcgccagcg gggaaggcgc ccgcgacggc gggcgaggcc aggtggcaga ggagggcgtc
     3961 gtcagagagc gagtagaacc actcggggcg gatcacgcct gcggcgacgc catcctggag
     4021 cagccggcca aacatggcgc tggcgatccg ggccgtgtgg tgccagtaga tgtgccggaa
     4081 catcatgtat ttcgagaaga tcaggctctc cagcggggcc acacccttct cggtgatggc
     4141 gatccgccgg ccacccggac ccacgtccag gaccagcgat tcgatgaggc ggtcggtatc
     4201 gatgtgcccg tagggaacgc cgcaggcatc ggcatcgcgc atcaggtaat ccatcttgtc
     4261 gggatcgacg atgccgctga gcaggcgcgc cggaaggttg tcctccccgc tcgcgtcaac
     4321 cggcgcgatc gtgttcgcga cggatctcgg atccgagatc ccccactcct cgcgaagcac
     4381 cgtcgccagg ccgcccggtc gctctggatc caggtattcg cgggcgcgtt cttcatggtc
     4441 caggaaaagc ggctggccgg cgctgtcacg cagcggcagg tgatccagga gatgggcgca
     4501 ggggtagtgg ccgatatcat gaaggagtgc cgccgccagg aaaacgcgcc cttcatccgg
     4561 cgcgatccga ggctccccgg tctccggatc gttgttgtaa gggttggcca ggaggctgag
     4621 caacaactgg cgcgccagat ggtaggtgcc gatgcagtgc tccaatcgcg tgtgcgtagc
     4681 gccgggaaag gcgtagtgca cgaagaggag ttgcttcacc tgctgcaggc ggacgaaatc
     4741 cggatgctcg atcacccggc gtgccgccct tgaaagggga atatgccccc agatcggcag
     4801 ccgaacatag ccgccatctt gcgcgaaaag gaattgccag tcgccgctga ttctctccaa
     4861 gtgtccctcc agggatggcc acgctcgtcg tccaccatcc taccttatta tagcacaggc
     4921 ctgcctgcca tggcgcgcct cgctctctct cccggctggg attccgagat aagggatccg
     4981 aagcatcgaa ggcggcgcag aacccggcgc gcggaggtta accaccgcgc gtgggctggc
     5041 gatacgcccg gatatctcaa aacggcggtg gctgggaagc ggagaccaga aggggctttg
     5101 cctggaagca gcgcggagct tcgagctccg cgcgcgtggg tgttgcgcaa cccggcgcac
     5161 ggggattgat caccgcgcgt gctggcagcc ttctccatgc atgtccgccc tttatctcgg
     5221 aatcctagct cgcccgcagc gaggcgcggg ttggcacgcc ggctacggct gccccttacg
     5281 aatgaatacg gagcgataga ccaccacctc gtcaccctcc ccaaactcgt agagggagac
     5341 catgcgccgg ccatcaccat ccacatccgg gaagtcctca tccttcaccg ggcggtccag
     5401 aaggatgctc tctttctcca cactcaccac gcggtagcgt gccttcaaat cctcgccggt
     5461 cgctaccgtt ccattgtaat agcccggctt gaagccgctc gccgcttcaa ccggcggcgc
     5521 ggtctccgtg atcaggcatc gccggtcggg cgacaccgac atcagcttcg agcggaagag
     5581 gatgctgttc agatcgaggc gcgcttgctt gccatcgtcg ctcgcctcct ccaggtggta
     5641 ggcgctccgg tgattgcctc cccggaggta gatgagctgg cctggccgga cgaggtcgcg
     5701 cgggatggga tgctcaagcg tgatctcgct ctttcgtaga tcggcccgga tgacgcgcga
     5761 gcggaacgcg ggcggaatct cggaaacccc ctcgttcccc cggcgcagcc gctctccgtt
     5821 caccaggtga agggcgcgca agacgccctc cttctccgac cagtagccga agctaccccg
     5881 gaactccagc ccgccggcgc gcagaagcgc gtccggattc caggagtaga agaaagtgtc
     5941 cacctggccg ccggccagtg tcacccgaag tgcaacgggc gcgaactccc cacccgggcg
     6001 ggcgccctcc accgggaggc gctctacctt ctggatgaac ggctcgccct cgtagggctg
     6061 ccagagcgcg ataaactcgc tggcgccatc ggctccctcc tcctcagcaa agaggtagcg
     6121 gatcggcggg atatccggct tcgcgcagcg cgccgtgatg tagcgccgcc ccggccgtcc
     6181 gagatgggtg atccgcaggc gcactggttt ctcctggcac ttccaatcgg cccatacatc
     6241 gccatcgaag gctgctgcct gtggctcgac catgttgttg gccagaccgc gggtgatgcc
     6301 gctgactttg tagacggtac ccttctcacc gaaggtgagg ctcgattgaa agtcatcatg
     6361 gccggggccg tggaagcaat aggtgcgcgt cgttccgccg gccaggcgca ggatatcgag
     6421 gaggtagaca ttcccgccgg gggcatccgc caggcagacg gcccgccggt agacgcgatt
     6481 tgggaagccg tcgggcgccg cgctgacgtc cgcgaaggag gcctccgggg ctcccgcgaa
     6541 cagctcgaga tcaccacggg agatggccga ccggtactgc cccgaccggt caatcattcc
     6601 ggtgttatgg tgggcgacgt gagcggtatc acccatcggg tgggcccagc aaggataccc
     6661 cagctcgggg gcgacgctca accccttggc aaacatctcg atgttgaggt tgtcctggtg
     6721 gtgatgcccg cgtccatagc catagcgtag agcgattccg gcgcgctcct cctcctgcgc
     6781 cgcgtgggcg cgcgattcca ggaagacgaa acccacgccg tccaggacac ggctttcgag
     6841 gggtggctcc ggcccggcct ttttcgcatg agcagcgacc tgcggccaga tatcctcctc
     6901 gaaaagcccc ggatcgcgcg ggcccaggcg gaagagagcg cgcgccaggc gatccgtggg
     6961 ccaacgccgg taagcgcgct cataggtcgt gcgcacggcc gccgggatcc ccttcggata
     7021 cgccgccgtg ttgcccgcgc cactatcgcc atagctggga ccgaagcgac cggcgacgac
     7081 gaaatcgagc caggtgtcga actcggcgcg cagcttgggg tagagcgtct cgtcatacag
     7141 gttgcaccgg gctttccagc gcggagagtt cacttccgac agaatctccg ccatgtctga
     7201 tttcgagttc aggtaatcga cggcatagcc cagggccgag atccagaccg ggccctcgcg
     7261 cgtggtcgtg ttcacaagcg tctcgtcgtc gaagccgccc atgttggtgc cgctgttgta
     7321 ggcatgcgtg aacagctcct cgatccagcg gtcacttacc gctcccatat cagcgcagac
     7381 ggcgaactgt gccatgtctt cctcgcgcgc gcccatgttc ccgccggaga gctcccggcg
     7441 catccaatcc cagccgaaca gctgcaccac gtaggtgtcg atcagcgcgc gcacatccac
     7501 tggcgtgcgg atcgccgggt ccttcgtgtg gagaaagtca gcgagcgcct gatcctcgcc
     7561 aaggaggtca aagaccgcgt cataggcgcg cacgagtggc accaggatct cattgcgctc
     7621 ccagttccca tcgagaagca gacgccctgg gcgcagatag tgggagtcca cctgctgcca
     7681 gcgcatgttc atcccgggat agacataggc gacgcgcgcc aggatcaccc ccaccttgtg
     7741 cgcggcagcg cggtcgccga agaggagggc gcgttgcgcc atgcggagca cctctccccc
     7801 gaagttctgc cagacggcgt ggtggttgta gtaggccacc caccccgcgt aatcgtcgcg
     7861 cttgccggtg cccggatccc atccccagcc atcatctggg taatcgccgc tggtgaagtc
     7921 gccacgggca taatcgttgg tggggaaaag ctgcttgcat acggggcact gtgccttgaa
     7981 gggggttgcc gtgttgttcc gccagggata gaaggcgttc acgcggtaga tcgcttcacc
     8041 gcacttgggg cagggccagt tgctatagca ctcccgcggc acgttccatg aggggaggct
     8101 ggcgaagatc tcctcgtcgc tcttcccgtc atagcgggag gggcggacca gctcctcgcg
     8161 cagctttggg taacgctcca ggttctgcct agccagggcc accttccggt cggtgtagta
     8221 ggaccggcct cgcttgcgcg ccgtgggatc gatcccgggt accacgcgaa tctgctcctc
     8281 gatatcttga cggtaggtgc gcaccgtcaa cgggagcgag aaggttggcc cgttggccgg
     8341 agcgagcgtg atcgagccct tctcgatgcc ccggttggcc cggaggtaga agcgcgtgcg
     8401 cgttctcccg cgtcctggct tcgtccggtc gaggagctgg acgccctccg ggagggtgat
     8461 cttgacttcg agccccgggt cgaacggggc ctctacccag aaaacctgct ccatcaccgg
     8521 caccggcgca ggcccaatgg tgggctgatc cgaccggcga tagggatcgg ggaggtccgc
     8581 ggcggccccc caggcgcccg tcgcgatgac gagcaggagg ggaatcaggt ataagtacga
     8641 gtgagagaga gccatctatc acctctgccg aggcggcgag agacaggctg ctcccttctc
     8701 ccgcgtccgg caccgtgtaa tccaccggca gggccggctg atcccctggt tcgatgcgga
     8761 tcctcacgcc tcctcgttgc ccccgcccgg gaggatcgag gggcagagga cacgcggcac
     8821 ggatgccagg gcgcgacttg ctggcgcatg gccggaagcc cggggggcgc gcatgggcac
     8881 gtctgccggg gtgacgcttg acgctcccgc cccaggtggt tacaatgtac ccaacaatgg
     8941 gctggccaca tggctggcgc attccactga tttgcccctg ccgcggggcg ccagggaggg
     9001 cggaatggct gaggagcagc gcctggaatg ggaggcgctt cccaccgagg agttggtgga
     9061 agagctggag tacgtgggtc tgagattgcc cttagggttg gtcgaggaga tcctccgccg
     9121 cggcaaggac gccttggagc cactgggacg ccgcgtggcc gatgccagcc tgtgggagag
     9181 cgacggcgat gagcggtggg caccgctgca cgcgctccac ctgctggggg ccatcggcga
     9241 gcccgcagcg gtcggcttca tcctcgatgc cctgcgcatc aaccccgagc cagatgagat
     9301 catcgagaac acgcccacag tactcggtca cctcgggccc gaggtgattc ccgagctctc
     9361 gcgcttcatc ctggacgagt cggtggatgg gctgatgcgc ggcgttgcct gcgacggtct
     9421 agtctccatc gcggtgttgc acccggaaca tcgcgacgag gtggcccgct tcctccgccg
     9481 cttcttggaa gaggccgcac agcacgaccg cgaggctgtg acggcagcga tcctcagcct
     9541 ggttgagttg cgcgataagg agtcgcggcc cgccatcgcg gcagcgttcc gatccgggca
     9601 tgtggatacg gacctggtca ccctcgagga tgtgcatgag gcgttccagc agccggagcg
     9661 tgtccgtgac gactggcact tcaccggtga tccgctggag ttcttccagc cggagcggct
     9721 tgagaagatg cggagcaagg agtgagccgg gcaacggggc acgagcacaa tggatgacct
     9781 ggtcgagcag ttcctcggac atcttgcctc tcatcggcgc ctctcgcaga tgacggtgaa
     9841 gtcgtatgcc gaggacctgg cgcatttcct gcggtttctg gaatcgcccg atgtggcgtg
     9901 cgaggagtgg ggggcggtgg atgtgcaggt catccggcgc tatctgggcg cgctccagga
     9961 tcaggagtat cgcaagacga ccatcgcgcg ccggctctcc tgcctgcgga gctttttccg
    10021 gtacctggtc cggcgccggg tgattaccgc ggacccgatg gcgggtctca tggcgccgcg
    10081 gctggagcgt cgacttccta aggtggcgaa ccgggagttg ctggatgcgc tccttgaggc
    10141 gccggacgag acaacgcccg ccgggctgcg cgaccgggcg attctggagc tgctctatgc
    10201 caccggggtg cgcggcagcg agctggaagc gatcgacgtg agcgatgtgg agttcgggcg
    10261 ccgggagatc cgggtgttgg ggaaggggag caaggagcgc gtcgtgatct tcgggaaggc
    10321 ggcccaggaa gcgctggcgg agtatctcag ccgcggccgg ccccaccttt tgaaggagaa
    10381 ccgagacgag ccggcgctct tcctcaaccg cttcggcaag cggttgagcg tgcgctcggt
    10441 gcgcgacatc ctggagaagt acctgatgca ggtggctgcg gagcttcacc tgagccccca
    10501 ctccctgcgc cactccttcg cgacgcacct cctggatgga ggcgcagacc tgcgcgcggt
    10561 gcaagagctt ctgggacaca gcagcctggg gaccactcag gtgtacacgc acgtgagcaa
    10621 ccagcgcttg caggccgcgt tccggcgagc gcacccgcga gcgtagcgcg gcgtcgacac
    10681 caaagggatc ttctggcacc agtgcgttta acggcgcccc cggcgatggg agcacgcgct
    10741 atcggtttgc cagggggctt tatgcggtgc gtgccacggg tataaggaga acgaatgaag
    10801 gtgcaccata ccacgatcgt ctgcgtgcgg cgcgacggca agaccgcaat cgccggcgat
    10861 gggcaggtga cgttcgagaa taccattatg aagcagggga gccggaaggt gcagcgcctg
    10921 gccggcggga aggtgctggc tgggtatgcc ggcgccgctg cggatgccct aacgctgatg
    10981 gagcgcctcg aggccaagct ggatcaatat gcggcgaacc tggagcgcgc cggtttcgag
    11041 ctggtcaagg agtggcggac cgaccgcgtg ctgcgccggc tggaggcgct ccttgtcgtg
    11101 gccgatgagc gcggcatgtt tgtgatctcc gggggcggcg atatgatccg accggatggc
    11161 gacgtgatcg ccgtcggctc tggaggcgga tatgcgctcg cggcggcgcg cgccctgatg
    11221 aagcatacga gcctgagcgc ggaggagatt gcccgcgagg cgctcgggat cgcggcatcc
    11281 atctgcgtct acacgaacga gaacatcact gtcgagcttc ttggatagca ggcgccgttc
    11341 ggcgcttcgc tcgcccgaag ccgcggcggc ttgctggcgc cgccaagggg ggatagcatg
    11401 aggcagttga ccccacggga gattgtggag gagctgaaca aatacattgt gggccaggat
    11461 aaggcaaagc gcgcggtggc gatcgcgttg cgcaatcgtt accggcggcg cctgcttccg
    11521 gaggcgcttc gcgaagaggt gattccgaag aatatcctga tgatcgggcc gaccggcgtg
    11581 ggcaagacgg agattgcccg ccgcctcgcc cggctcgcga acgccccctt cgtgaaagtg
    11641 gaggcgacca agttcaccga agtgggatat gtgggccgcg atgtcgactc catggtgcgc
    11701 gacctgaccc aagtggcgta tcgcatggtg cagcaagagc ggattgagga tgtgcgtgag
    11761 gaggccgagc gcctggctat cgagcggatt gttgacctcc tggagcccct gccccggtcg
    11821 cgccaggagg ggagcgcctc cgatttcctc atggaggcgg cacggcagtt tatgggggcg
    11881 cgcgcagggg ggaccacgcg gccagcgcct gtacaagaac cctccgcgtc tcccaacctc
    11941 gaggcccggc gcgcggagct gcgccaaaag atcattgccg gcgagctgga tggcgatcgg
    12001 attgagctgg aagtggagga gacgcacgcc cccttcatgc aggtcttctc gcagcaaggc
    12061 ctggaagaga tggggatgga cctccaggga atgctgggga atatcatgcc ccggcgcacc
    12121 aagaagcgca acgtctccat tgccgaggcg cgcgagatcc tccagaagga agaggcggag
    12181 aagctcattg ataacgatga ggtggtgcgc gtggcgatcg agcgcaccga gcaagacggc
    12241 atcatcttcg tcgatgagct ggataagatc gccgggcgtg agaagggcgc cggcccggat
    12301 gtttcacgcg agggcgttca gcgcgatatc ctcccgatca tcgagggtgc gaccgttgtg
    12361 accaagtatg gacccgtacg cacggatcac atcctcttca tcgcggcagg cgcgttccac
    12421 atgtccaagc cgtctgatct gatcccggag ctgcagggcc ggcttccgat ccgcgtagag
    12481 ctggaatcgc tgggagaaga ggagttcgtt cgcatcctga gcgagccgga gaacgcgctc
    12541 accaagcagt acgcggcgat gctcgagaca gaaggcgtgc atgtcgagtt caccgaggag
    12601 ggcatccgca ccattgcctc catcgccgtt caggtgaact cgcgcacgga gaacatcggc
    12661 gcccggcggt tgcacacggt gatggagcat ctcttcgagg agctctcttt cctggcgccg
    12721 gatatccagc cggctacggt gcgcatcacg gagcagtacg tgcgcgaacg cttggaaggc
    12781 atcgtcaaga acgaggatct gagccggtac atcctctaag cgctcgcacg gcgaaagccc
    12841 gtggggcttt ggccctggcg gggccatatg ggctgcttag agagctctga tggggcgaac
    12901 ccgaccgcga agaccgggct cgcgaaacgc gaaagaacgg atggaccgcc ctcggtagac
    12961 gactggcgtc ttcctccctg ggtcacgaat ctcgtgaagc gggggagggg ccccgagctg
    13021 ggattgctcg ggctcgcacc gcccctcgcc cgcgcctccg atcactccac cacgttctcg
    13081 atggagccaa tccgctcgat ggtggtgcgc acgcggtctc ccggcttgag gaagatgccg
    13141 cggccgctgc cgacgcccgc cggcgtgccc gtggcgatca cgtcgcccgg ctccagggtg
    13201 atgaactgcg agatgtagtg gatcagctcg gcgacgggga agatcatctg ccccgtgttg
    13261 ccgttctgca tctccgcgcc gttcactgtg agcgtgacct gcagccgctg gggattgggg
    13321 atctcatcgg cggtgacgat ccaggggccc attggcgcga aggtgtcgaa ccacttcccg
    13381 ttcagccagt cgaagaactt gtcccactcc tcggatttgg agcgctcgcg gatcttgagc
    13441 gcgcgctcgg agacatcgtt gatcacggtg tagcccacca catagctcag agcatccgct
    13501 tcggcaatcg ccttgcctcg ccggccaatg acgactgcca gctcggcctc ccaatcgatc
    13561 ttattgccta ccggaggtat
//
LOCUS       DAOSCV010000007        13554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3078_length_13554_cov_3.388103, whole genome shotgun sequence.
ACCESSION   DAOSCV010000007 DAOSCV010000000
VERSION     DAOSCV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3078_length_13554_cov_3.388103"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PLY56_00395"
     CDS             complement(<1..778)
                     /locus_tag="PLY56_00395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOJ19969.1"
                     /translation="MSNRGDLETMAREEYDLLVIGGGILGCGVARDAAMRGLRVALVE
                     KEDLGYGTTSRSTRLIHGGLRYLELFDFALVHEGLHERQHLLQIAPHLVRGIPFLTPS
                     YVGDRWGPLFLRAGMVLYDLLSLGKSLPSHRMLTPAQVRELEPGIRSEGLRGGALYYD
                     AQVAYPERLCVENALCAQKHGAQIANYAEARQLLRDGQRVCGALVEDRLTGATHPIRA
                     RLTVNAAGPWADEVARLASPEARPVLRRTKGIHLLVPSFTR"
     gene            1111..1596
                     /locus_tag="PLY56_00400"
     CDS             1111..1596
                     /locus_tag="PLY56_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19970.1"
                     /translation="MLRRWWFVALVPALLYAAPAQAQGGWGWGSRVAPETEKEKAYVE
                     DARKLRSELWQKQRELWDLQTQEKPSASALKAKETEVNTLRRKLSDLNYKNRELRWQM
                     MDRARQNAARSWMGRGVRGMGPGAGMHPRGMGGRGAWIRRPGSWGGCAAGVCPWKAPA
                     R"
     gene            2314..4473
                     /locus_tag="PLY56_00405"
     CDS             2314..4473
                     /locus_tag="PLY56_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ19971.1"
                     /translation="MNRSSDRSEEARAGALHAYGILDTPAEEVFDGLAMLATQVCATP
                     MALLGFIDRGRVWIKAKVGLDVVELPRGGFCARAMEQDGPLIIADAAMEPDLASDPFL
                     NGQVKARFCAAVPLRDRQGYTIGLLTVFDRVPRRLRQDQAQGLQALGHQAMALLDARC
                     ELSKRARVAHAPGDPKPASHSSRGTLPDARFRKAFDASPELMVISALSDGRYIEVNET
                     FLRVTGYRREEVIGRTASEVNFLVDPWERSQIRQLLRQRGAAHNMEARFRLKSGEERV
                     GLLSATTIHLGYERYVLYVVNDITERERAEEEQKRADARMQRAQKLESLGILAGGIGH
                     DFNNILMGILGNASLALEQLPDDAPARYHIREIEKSALRAADLTAQMLAYAGEGETAR
                     EPVNLSKLAEEMGGLLHSAVSKKATLRCTYAANLPVVLADPAQMRQVLMNLVTNASEA
                     LGDDEGAIRVSTGILQADRVYLAHAYPDPELPAGTYVYLEVSDTGVGMDAQTRARIFE
                     PFYSTKGPGRGLGLAACLGIVRGHGGGILVDSRPGHGTTIRLLLPCAKEQPPVETSVR
                     PSTLQAAAPTVAPRPSAAQREVSGKIADTWRGSGTVLVVDDEDVVRAVARMMLEFVGY
                     TVLVASDGVDAVEVFRQHADEISVVLLDMSMPRMNGEQTFDAIRAIRPDARVILSSGY
                     QERETIERFSGKGLAAFLPKPYQFQALLRKIREVTQC"
     gene            complement(4611..5132)
                     /locus_tag="PLY56_00410"
     CDS             complement(4611..5132)
                     /locus_tag="PLY56_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19972.1"
                     /translation="MLKHVAAITSVLGLALLASPASAGRYDYYRESGAGTEWTKKVRF
                     VAAAGVNSSGIINFLTFRDGSSESGWSWQLAGSYQLEQPYFVEAGLMGWTIGRENQES
                     AKKFGVAASAGVQLHATEIGAGFVPSGARIFVRHFFDGDTDGKGTMGQAELVFPTTSG
                     AHSYLGLSLGYGF"
     gene            complement(5369..6640)
                     /locus_tag="PLY56_00415"
     CDS             complement(5369..6640)
                     /locus_tag="PLY56_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19973.1"
                     /translation="MRNSFSWIKALLWAGILTIASTPASAQGGRVHRLLPAHFMLGLA
                     NGSSELEWLRNARVPIEMRYQYLSGGVNTGQGWTTWRPKARYAADYVRDSRAVGAIPV
                     FTYYNMLHSRPARGSSESEKVYRNLNNPETMRAYYEDFKLLMQRASAAGDRAVVHVEP
                     DLFGYLQRDYADGGRRSAREVSAAVASTGMQELRGLPNTGQGFGRALLRLRNRYGRGV
                     LLAIHTSDWASGTDIGSSQNPRVDARGIGVRTGRFLRGFGEGWNLVFVSPSDRDAGWM
                     DRYRPGGTHWWDPANRAFPNFERYRQWVDGVSTGADLRVVLWQVPIGNRRYRTMNNTE
                     GHFQDNRAEYFLESRNHIADYVRAGAIAILFGAGAAGTTHYDDRQRDGITNPPPINGN
                     TLISRYTDDDGGYLRLAITRYYRVGSVVVPR"
     gene            complement(6680..9955)
                     /locus_tag="PLY56_00420"
     CDS             complement(6680..9955)
                     /locus_tag="PLY56_00420"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ19974.1"
                     /translation="MILARALILMLLAASCVAPAMAEVVPFPDADMESPGRDAWETYG
                     TPVSIEKVRQAHSGQWALRVQTDNKETMGGGFEGVSRTLGELRQGDRVRVSFWLRPLA
                     GREVWIGMGRTSFEQIWRPFDTDWMQVVCDFRARADGTHSIWITQQGAANDFLLDDFR
                     VERIPRLALGQAPASQRVAIGDSAGRVWLCRETGALCGIENLATGETVAPLGERQPLF
                     SLSVIDKERQTETIGFDRATLQQFAAAPDGSRATLSYAVRGLPIRVTLQVMRSAPGEI
                     AFSAEVENRSDRSILEVTYPLVYSVRPAADPKALTLVHPYLCGQIQRDALRSAGCDSV
                     YPGRAVMGWFDLYGEKGGLSLSTRDTTRTGTRLTALPAAGDTFDMSLSQEVVIRPNTT
                     WTGPRHVLLIHAGDWHIAADRYREWARQWLGTPDVPRWIQEADGWVLMGVQSGVPFWR
                     IPDVYRSAEWMGVDYLHVQGQAVDNMWFDADGKRHGHAITFLYPSPRFGTPEQLRQAV
                     RDIHSRGGHVMFYFLYERWTPSHSVSDDLGTAPRSAIPPEYPIPGLDFYEKNALVERP
                     GARPPKEHPFMVHRMMCLGSPGWQEWMRHWAIDVYAKRFEADGFYWDVMGRNGPFRCF
                     NEAHGHQGENRWAAGSAAVLERTVREGRAINPDYSCAIEGCQDALAPWVGFHLMSGAT
                     QTPEVFRYTFPELCLVDGFSNHYWKWTQVEKARRVFLCGERFDLHGYHAQVRKLVWLR
                     KRLRPFIDWPARFVDTIGVATSDPAVQARRFVRDDGGNRAVLITLLNEGLKQDATITV
                     DLAPMRAITTAHLFTLDGRIRPLQSTPTGQGKVSFTAPAEPVAGVLFVDTVDPALRVV
                     PVLEQIQVPDRDGLELTLFYPAGRKEKCDVRLRAPAGVTLRAVDSPRPEESPAVERRA
                     WEPEGGLASLQGWEKIHADLQWPGGKTSVWSLLCPPLVNGDMERLRPDGHLAYWPASP
                     SVEEPAQGKHCLKLEDQGQGDTGFVSTLTPIKPGTRYRLSARIKRDTAGSDTRVAMIE
                     YEESSKFHVSASVGAGGKANEWQEFTTEFTSHPSPRSSAVYLYRGNSKAAWFDQIELV
                     ELK"
     gene            10410..11036
                     /locus_tag="PLY56_00425"
     CDS             10410..11036
                     /locus_tag="PLY56_00425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ19975.1"
                     /translation="MTRIYLIRHGEVLWNREHPAYCGHTDLELNERGREQAECVAQRL
                     AGAELSAIYCSDLRRARDTAAAIAARHGLEPVADPALREVNYGEWEGVSEEDVRAGWP
                     DHYAAWKRDAEKVRIPGGESFGELRDRLVPALTAIAERHAGENVAVVAHKSSNRVFLC
                     AVLGLSPSLYRRIGQENAAVNLISYEGCRWRVDLVNDTCHLRLPGNSG"
     gene            complement(10993..12549)
                     /locus_tag="PLY56_00430"
     CDS             complement(10993..12549)
                     /locus_tag="PLY56_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ19976.1"
                     /translation="MLKPSNHPAVNREDRASLEWALIAGLIVVAAALRFWRIGVESLW
                     LDEAASWSFARLPVAELLTRNADPGNPPLYYLALGQWLALFGDSEIALRSFSAVAGVL
                     SVPVIYLLGRELFGRRAGALAALLLAFSPIHLFYSQEARQYTPAMFLGLVSAWLFMRA
                     VKTDRWAHWLGAAFFAALAFYIHYSAMLIPAFQGAYALVAARRWRAVGAAALFGLLCL
                     PGVVLYLAPTFLLRDGYQFWQGHVTPGALARMGNKLFGKPTSASDPPEIQAACALLLG
                     IAAVYPLWLAGRSAKREDTDGGRPLLFSLLYLLVPMVLFIGLALMKPVWQERYLLVAL
                     PGYLLVLAWVLTRQQGARRVLLATILVLLHAGGMKAATREWQKEEWREAVRMMQTAAR
                     SARNPDTEVFLTMPFLHLPFDYYSRGNPPRTGYPRGRAPLTPADTAELDALARRAAQR
                     GTIFVIHGVAGDGDPDLLLVRRLAAIKGTGTFRRFVGMRVFAFGEGAPALGGIPVTPS
                     SLAAGDDRYR"
     gene            complement(12658..13017)
                     /gene="acpS"
                     /locus_tag="PLY56_00435"
     CDS             complement(12658..13017)
                     /gene="acpS"
                     /locus_tag="PLY56_00435"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOJ19977.1"
                     /translation="MIDGLGTDIIEIARIEAAMNRSPAFSKRLFTEAEVSYCSARGRP
                     AQHFAGRFAAKEAVIKALGRAVPWREIEILNDERGKPVCTLHGKAREIAAGRRVLVSI
                     SHCETYSTAVAIALLEA"
     gene            complement(13029..>13554)
                     /locus_tag="PLY56_00440"
     CDS             complement(13029..>13554)
                     /locus_tag="PLY56_00440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF013626.2"
                     /note="catalyzes the formation of glutamate 5-phosphate
                     from glutamate in proline biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ19978.1"
                     /translation="ARVAHLAGGPGSAVGSGGMVTKLQAARIAARCGATTVITHGRRD
                     GVICALLEGERLGTVFPPSEHRLRARKRWIAFGARPRGAIIINEGARRSLVEEGHSLL
                     PAGIVAVEGRFQAGDLVRIEDEEGRELARGLTNYASQDLAAVRGLHTDEVANYLGACD
                     FEEAVHRDNLVVNV"
BASE COUNT         2339 a         4323 c         4659 g         2233 t
ORIGIN      
        1 gccgtgtgaa cgaggggacc aggaggtgga tccccttcgt gcgccggagg acgggccggg
       61 cctctgggga agcgaggcgc gccacctcat cggcccaggg gccggcggcg ttcactgtga
      121 gccgggcgcg gatgggatgg gttgcgcccg tcagccggtc ttccacgagg gctccgcaaa
      181 cgcgctggcc gtcgcgcaac agctggcgcg cttccgcata gttggcaatc tgggccccgt
      241 gtttttgggc gcagagcgcg ttctccacgc acagccgctc tggataggcg acctgggcat
      301 cgtagtaaag cgcgccgccg cgcaggccct cgctccggat tcccggctcc agctcgcgca
      361 cctgcgcggg agtgagcatc cggtgcgagg gaaggctttt ccccagcgag aggaggtcgt
      421 aaagcaccat gccggcgcgg aggaagagcg gaccccagcg gtcgccgaca taactcgggg
      481 tgaggaaggg gatgccgcgg accaggtggg gcgcgatctg gaggaggtgc tgacgctcat
      541 gcagcccctc atgcaccagg gcgaagtcga acagctccag gtagcggagc cccccgtgga
      601 tcagccgcgt cgatcttgat gtcgtgccat agcccaggtc ttccttttcg acgagggcca
      661 cacggagccc gcgcatcgcg gcgtcgcgtg ccacgccaca cccgaggatg ccgcctccga
      721 tcacgaggag gtcatactct tcgcgcgcca tcgtctccag gtcacctctg ttgctcaccg
      781 atgccaccct ccatctcgcc tctttctatc aggttccgta tcccgggttg ttttcctgga
      841 atcctcgctt accagctcgg gaacgcattt gccggtggct cgtctggtta gtagaggccg
      901 ctgcactggc attgggatga tgccatgcgc cgcttccggc agcggccaca gatgtgttga
      961 atgatcggga cctcccgatt caggtccaag gcaaaacacc aagaagtcgc agccccattt
     1021 gcactcgtcg cgcggcatcc gacgctgcgc ggtcgcgggt tggggaagga gacggcttct
     1081 gcacgctgct tctgaggaag gagagaggac atgcttcgac gatggtggtt cgtcgcgctg
     1141 gttccagcgc tgttgtatgc cgcgccggcg caggcgcaag ggggttgggg atgggggagc
     1201 cgcgtcgccc cggagacgga gaaggagaag gcctacgtgg aggatgcgcg gaagctgcgc
     1261 tccgagctct ggcagaagca gcgcgagctg tgggatctgc agactcagga gaagccgtcc
     1321 gcttctgccc tgaaggcgaa ggagaccgag gtgaacacgc tgcgccgcaa gctctcggac
     1381 ctcaactaca agaaccgcga gctgcgctgg cagatgatgg atcgcgcgcg tcagaacgcg
     1441 gcccggtcgt ggatggggcg gggtgtccgc ggaatggggc cgggcgctgg tatgcacccc
     1501 cggggcatgg gcggtcgtgg cgcctggatc cgccgcccag gcagctgggg tggatgtgcc
     1561 gccggtgtct gcccctggaa ggcgcctgcg cgctagcaaa gggctcagcc gatagtggac
     1621 aggcaggatt gcaggcggtc ctgcctgtcc gaagatcaaa tcgggaacac cccagggccg
     1681 tgctctctgc tgtctcctag gaagagcacg gtcctggggc ttcggcttgc gccggccaca
     1741 cgagaggggc ggcaaaaggg tgtggcgggg ggagcagagc ccgtctcgtt gcgaatgcac
     1801 tttgcctctc tcccggaggg atagtccggg caagggagga tgccggtggc ccgcccgacg
     1861 aaacctcatg ctcacacttc cgaaactctc attaggtaat tataggtaat tataggtgac
     1921 ttccctattg acccaaaggg gcgtgtcccg gtatactagc ctccatacct accaggagaa
     1981 gagcagccgg tcgtagagct ccggggggca gcactggcca ctggcatgcc agacgcgggc
     2041 ggcccttcac catacgagga tgctcctgca gaagggccag cctatccatg ccgagaggga
     2101 tgaggcagcc gccgttctta agagggtgcc cttatcgccg ggccgggcat gaaacgacgg
     2161 atggggatgt ctctcgcgcg tctgtctcgc actctactgg aaggtggaaa gatcaactcc
     2221 aacagggaat cgcctaccgg ctctgtgacc gccacggagt atcttccagg gattgggcac
     2281 ggcgtgttcc cggagagtag gaattccgag cggatgaaca gatcgtccga ccgcagtgaa
     2341 gaggcgcggg ctggcgccct gcatgcctac ggcatccttg atacgcctgc cgaggaggtg
     2401 tttgatggcc tggcgatgct ggccacccag gtgtgtgcca ctccgatggc ccttctcggg
     2461 ttcattgacc gcggccgtgt atggatcaag gcgaaggtcg gtttggatgt tgtcgaactg
     2521 ccgcggggcg gtttttgcgc ccgcgcgatg gagcaggacg gcccactgat catcgccgac
     2581 gcggccatgg aaccggatct ggcgtcggat ccgtttctga atgggcaggt gaaggcgcgt
     2641 ttctgtgctg cggtgcccct cagggatagg cagggctata cgatcggtct gctcacggtg
     2701 ttcgaccgcg tgccgcgccg gctgcgccag gatcaggcgc agggcctgca ggcactgggc
     2761 catcaggcga tggccctcct ggatgcgcgc tgtgagctct ccaaaagagc ccgagtggcg
     2821 cacgcaccag gcgatcccaa accggcaagc cactcatcgc gcggcacgct cccggacgcg
     2881 cggtttcgca aggctttcga cgccagcccc gagctgatgg tcatcagtgc gctgagcgac
     2941 gggcgctaca tcgaggtgaa cgagactttc ttgcgcgtga cgggctaccg gcgcgaggag
     3001 gtgatcgggc gcaccgcgtc tgaagtcaac ttcctggtcg acccctggga gcgctcgcag
     3061 atccggcagc ttttgcgcca gcgtggggcg gctcacaaca tggaggcgcg cttccgcctg
     3121 aaatcaggcg aggagcgcgt cgggcttctc tcggccacga cgatccacct gggctatgag
     3181 cgctatgtgc tctacgtagt caatgatatc acggagcgag agcgggccga ggaagagcag
     3241 aagcgcgcgg atgcgcggat gcagcgcgcg cagaagctgg agagcctggg cattctggcc
     3301 ggtggcatcg gacacgactt caacaacatc ctgatgggga ttctgggcaa cgccagcctg
     3361 gcgctggagc agcttcccga cgatgcgccc gcgcggtatc acatccgcga gatcgagaaa
     3421 tccgcgttgc gcgcagccga cctgacggcg cagatgctcg cctacgccgg cgagggcgag
     3481 acggctcgcg agccggtaaa tctctcgaaa ctggcagagg agatgggagg ccttctccat
     3541 tcggcggtgt cgaagaaggc gacgctccgc tgtacctacg ccgccaatct ccccgttgtc
     3601 ctagccgatc ccgctcagat gcgccaggtg ctgatgaatc tggtgaccaa cgcttccgag
     3661 gcgcttggcg acgacgaggg tgccatccgc gtgagcaccg gtatcctgca ggccgaccga
     3721 gtctatctgg cccatgccta cccggatccc gagctccccg ccggcaccta cgtctatctc
     3781 gaagtatccg ataccggggt gggcatggac gcgcagacgc gggcacgcat cttcgagccg
     3841 ttctactcca cgaagggccc tgggcgcggc ctgggattgg ccgcgtgcct tggcattgtc
     3901 cgcgggcatg gcggcggcat tcttgtcgac agccggcccg ggcatggcac cacaatccgt
     3961 ctcctcctgc cgtgcgctaa ggagcagcct cccgttgaga caagcgtgcg gccctccacc
     4021 ctgcaggcgg ccgccccgac agtcgcgccc cggccgtccg cagcgcagcg agaggtgtcg
     4081 ggcaagatcg cggacacctg gcgcgggagc ggcacggtgc tggtcgtgga tgacgaggat
     4141 gtagtgcgcg ccgttgcccg gatgatgctc gagttcgtcg gctacaccgt tcttgtggcg
     4201 agcgacggcg tggatgccgt ggaggtgttc cgccagcacg cggacgagat ctccgtcgtg
     4261 ctgctcgaca tgtcgatgcc gcgcatgaat ggcgagcaga cattcgatgc catccgggcc
     4321 atccgccccg atgcccgtgt gatcctctca agcgggtatc aggagcgaga gacaatcgag
     4381 cgtttctctg gcaaaggcct ggcggcgttt ctcccgaagc cctaccagtt ccaggcgctc
     4441 ctgcgcaaga tccgcgaagt cacgcagtgc tgagcggctt gtggaggtga gatccgctcg
     4501 ggagagcgcc cgcggccatc catcacgggc gctccacgag aggggaatga ggggcgcccc
     4561 cgagcccgtg tgaggagacc ccgggagcac gccccaaatg aggcggtttc ttagaaaccg
     4621 tagcccaggc tcaggcccag gtagctgtgc gctccggatg tggtggggaa gacgagctcg
     4681 gcctggccca tagttccctt gccatcggta tcgccatcga agaagtggcg gacaaagatc
     4741 cgggcgccgc tcgggacgaa cccagcgcct atctctgttg catgaagctg cacgcctgcg
     4801 gaagccgcca cgccgaactt cttcgcggac tcctggttct cgcggccgat ggtccagccc
     4861 atcagcccgg cctcgacgaa gtaaggctgc tcgagttgat acgagcccgc gagctgccag
     4921 ctccatccgc tctcggacga gccatcgcgg aacgtgagga agttgatgat gccgctcgag
     4981 ttcacccccg cggcggcaac gaagcgcacc ttctttgtcc actcggttcc cgcgccggat
     5041 tcccggtagt agtcgtacct tccggcgctg gcgggggagg ccaggagtgc gaggccgagg
     5101 acggacgtga tggcggcgac gtgcttgagc atagcagggc tttcctttcg tatctcctca
     5161 gtatgttggc tcttgcaaag aacgcgcttc atccagtttc gacggtactg cagtgccgaa
     5221 tgttcccggc aaccgcaggc gcaaccgggc cggctggggg tcctcccacg tttgggccgc
     5281 atagcgcagc gtgcccaagc gagacctcgc tctggctggc ggtcgggatc cctggccggg
     5341 cattgccgac ttaactatcg gcatttgctc agcgtggcac aaccacggag cccactcgat
     5401 agtaccgggt gatggcaagg cgcaggtagc caccgtcatc atcggtatag cgggagatca
     5461 gggtgttgcc attgatgggc ggcgggttgg tgatgccgtc gcgctgccta tcgtcgtagt
     5521 gggttgtgcc ggcggccccg gcaccgaaga ggatggcgat ggcgcccgcg cgcacatagt
     5581 cggcgatgtg attgcggctc tccaggaagt actccgcccg gttatcctgg aaatggccct
     5641 cggtgttgtt catcgtgcgg tagcgacgat tcccgatggg cacctgccat aggacgacgc
     5701 gcagatccgc gcccgtggaa accccatcca cccactgccg gtaacgctcg aagttgggga
     5761 aagcgcggtt ggccgggtcc caccagtggg tgccccccgg gcgatagcgg tccatccagc
     5821 ctgcatcgcg gtcggatggg ctgacaaaga cgaggttcca cccctcgccg aatccgcgca
     5881 ggaaacggcc agtgcgcacg ccgatcccgc gtgcatcgac gcgagggttc tggctgctac
     5941 cgatgtcggt gcccgaagcc caatcggagg tgtggatggc caggagaaca ccccggccgt
     6001 aacggttgcg caagcgcaag agtgcccgcc cgaagccttg ccccgtgttt ggcaggccgc
     6061 gcagttcctg catgccggtg gaggcgaccg ccgccgagac ctcccgggcg ctgcgccggc
     6121 caccatcggc gtaatccctt tgaaggtagc cgaagaggtc cggctcgaca tgcaccacgg
     6181 cccggtcgcc ggcggcactg gcacgctgca tgaggagctt gaagtcctcg taataggcgc
     6241 gcatcgtctc cggattgttc aggttgcgat agaccttctc gctctcgcta ctcccgcgtg
     6301 cggggcgaga gtggagcatg ttatagtacg taaagaccgg gatagctccc accgcccggc
     6361 tatcacggac gtagtccgcg gcatagcggg ctttcggccg ccaggtcgtc cagccctggc
     6421 cggtgttcac cccgcccgag agatactgat agcgcatctc aatcggcacg cgcgcgtttc
     6481 gcaaccactc cagctcgctg gatccgtttg ccaggcccaa catgaagtgc gctggcaaga
     6541 ggcgatgtac tcgcccgccc tgcgccgacg ccggcgtcga tgcgatggtc aggatccccg
     6601 cccacaggag cgccttgatc cacgagaagc tgttccgcat gcctgggcgc ggtgcatccg
     6661 tggtgccact tcaagccgct cacttgagtt cgacaagctc tatctggtca aaccacgccg
     6721 ccttgctgtt gccgcggtag aggtaaacgg cagaggagcg cgggctcgga tgggaggtga
     6781 actcggtggt gaactcctgc cactcgttcg ccttgcctcc cgcgccgacc gacgcgctga
     6841 cgtggaactt cgagctctcc tcatactcga tcatcgcgac gcgcgtgtcg gatccggcgg
     6901 tgtcgcgctt gatgcgcgcg ctcagtcggt agcgcgtccc cggcttgatc ggagtgaggg
     6961 tgctcacgaa gccggtgtcg ccctggccct ggtcctccag cttcaggcag tgcttccctt
     7021 gtgccggctc ttctacgctg ggcgacgccg gccagtaggc gaggtggccg tcgggacgca
     7081 ggcgctccat gtcgccgttg acgagcgggg ggcagaggag cgaccagacg ctcgtctttc
     7141 caccgggcca ctggagatcg gcgtgaatct tctcccaccc ctggagagag gccaggccac
     7201 cctcgggctc ccaggcgcgg cgctcgacgg cgggcgattc ctcggggcgc ggggaatcta
     7261 ccgctctcag ggtcacgccg gccggcgctc ggaggcgcac gtcgcacttc tccttgcgcc
     7321 cggccggata gaagagggtg agctctagcc cgtcgcggtc aggcacctgg atctgctcga
     7381 ggaccggcac cacgcgcagc gcgggatcga cggtgtccac gaagagtacc ccggccacag
     7441 gctcggcagg cgctgtgaag gagactttcc cctggcccgt cggcgtgctc tgcagcgggc
     7501 ggatccttcc atccagcgtg aagaggtggg ccgtggtgat cgcgcgcatg ggggcaagat
     7561 ccaccgtgat ggtggcatcc tgcttcagac cctcgttcag gagagtgatg agcacggcgc
     7621 ggttgccgcc atcatcgcgc acgaaacgcc tggcttgaac ggcggggtcg ctcgtggcga
     7681 cgccgatcgt atccacgaag cgcgccggcc agtcgatgaa gggtcgcagg cgcttgcgca
     7741 gccagacgag tttgcgcacc tgcgcgtggt agccatgcag gtcaaaccgc tcgccgcaga
     7801 ggaagacgcg ccgggccttc tcgacctgcg tccatttcca gtaatggttg gagaaaccgt
     7861 ccaccaggca aagctccggg aaggtgtagc ggaacacctc cggggtttgc gtggcgccgg
     7921 acataaggtg gaagccaacc cagggggcta gcgcgtcctg acacccctcg atggcgcagg
     7981 agtaatcggg gttgatcgcc cggccctcgc ggacggtgcg ctccagcacc gcggcggagc
     8041 cggcggccca gcggttctcg ccttgatgcc cgtgcgcctc gttgaagcac cggaatggcc
     8101 cgttacggcc catgacgtcc cagtagaaac catcggcctc gaaacgcttg gcatagacat
     8161 cgatcgccca atggcgcatc cactcctgcc agcccggcga ccccaagcac atcatccggt
     8221 gcaccatgaa ggggtgctct ttcgggggcc tggcgccggg gcgctcgacg agggcgtttt
     8281 tctcgtagaa atcgaggcct gggatcggat actccggagg aatcgcggag cgcggcgcgg
     8341 tgcccaggtc gtcggagacg gagtggctgg gggtccaacg ctcgtacagg aagtagaaca
     8401 tcacgtgccc cccgcgggag tggatgtcgc gcaccgcctg gcgcagctgt tccggtgtgc
     8461 cgaagcgcgg gctggggtag aggaaggtga tcgcgtgtcc gtgacgcttt ccatcggcat
     8521 caaaccacat attgtcgacg gcctggccct ggacgtgcag gtaatccacg cccatccact
     8581 ccgcggagcg atagacatcc gggattcgcc agaaggggac tccgctttgg acgcccatca
     8641 ggacccagcc atcggcttcc tggatccagc gcggcacgtc cggggttcca agccactggc
     8701 gtgcccactc gcgatatcgg tcggcggcga tgtgccaatc gcccgcgtgt atcagcagca
     8761 catgtctggg gcctgtccag gtggtgttcg gtcggatcac cacctcctgg gagaggctca
     8821 tatcgaaggt gtcgcccgcg gcgggcagtg cggtgaggcg cgtgccggtg cgcgtcgtgt
     8881 cgcgggtgga gagcgagagg ccgcccttct cgccgtagag atcgaaccag cccatcaccg
     8941 cgcgtcctgg gtagacggag tcgcatcccg ccgagcgcag cgcatcgcgc tggatctggc
     9001 cacacaggta ggggtggacc agcgtgagcg ccttcgggtc ggccgccggg cgcacggagt
     9061 aaacgagggg ataggtgacc tccaggatgg agcggtcgga ccgattctca acctcggcgg
     9121 agaaggcgat ctccccgggt gcggagcgca tcacctgaag ggtgacccga atggggaggc
     9181 cgcgcacagc gtacgagagc gtggcccggc ttccatcggg cgccgcagcg aactgctgca
     9241 gcgtggctcg gtcgaagccg atggtctcgg tttgcctctc cttgtcgatc accgatagcg
     9301 agaagagcgg ctggcgttct ccgaggggcg ccacagtctc tcccgtggcc aggttctcga
     9361 tgccgcacag cgcgcctgtc tcgcggcaaa gccagacgcg gccggcggag tcgccgatgg
     9421 cgacgcgctg gctcgccggg gcctggccca gggccaggcg ggggatgcgc tccacgcgaa
     9481 agtcatccag caggaagtcg ttcgcggcgc cctgttgggt gatccagatg ctatgcgtgc
     9541 catcagcgcg cgctcggaag tcgcaaacaa cctgcatcca gtccgtgtcg aacgggcgcc
     9601 agatctgctc gaaggaggtg cggcccatgc cgatccagac ctcgcgtcca gcgagggggc
     9661 gcagccagaa cgagacgcgc acgcgatctc cctgcctgag ctcgccaagg gtgcgagaga
     9721 ctccctcgaa gccgccgccc atcgtctcct tgttatcggt ttgcacgcgc agcgcccact
     9781 gccccgagtg cgcctggcgc accttttcaa tggaaacggg cgtgccatag gtttcccagg
     9841 catcgcgccc cgggctctcc atatcggcgt ctgggaaggg gacgacctcg gccatcgccg
     9901 gcgcaacgca gctggctgcg aggagcatga ggatcaaagc acgagcgagg atcatcaaag
     9961 ccatcctttc gcttgggcag gcgtgtgccg ctgcccgctg tcgcctctgc tattagccgg
    10021 aatctggaga atctcctgcg ggagcacgcg tgctcgtgcc gcggcggcag ccaaaggcat
    10081 ttgagtgcct ggcggcgaat cctcctaccg gagccgggat tccgaaatac cgacggtggc
    10141 gcggaacctg gcgcgcggcg ccaaagcacc gtgctgcttg cgagcgaagc tctgccgagc
    10201 tggaccaggc aatactcccg ggtgttacag aatggcggtg gctggggggg agctgaaggg
    10261 ctgtgctgag aagcagcgcg gagcttcgag ctccgcgcgc gggcggaagg acgcgggatc
    10321 ccgaccgccg ggttcgaaaa ccactacaga gagcaggcaa ctgcctgcgc ccgtgttacc
    10381 ttggaatcgc ggctaccgga gggaactcat tgacacgcat ctacctgata cggcatggcg
    10441 aagtgttgtg gaaccgggaa cacccggcct actgcgggca tactgacctg gagctgaatg
    10501 aacgcgggcg cgaacaggca gagtgcgtcg ctcagcggct ggccggcgcg gagctatcgg
    10561 cgatctattg cagtgacctg cggcgcgcgc gcgacaccgc ggccgcgatt gcggcccggc
    10621 acgggctgga gccggtggcc gatcccgccc tgcgcgaggt gaattatggg gagtgggaag
    10681 gcgtctccga ggaagatgtg cgcgccgggt ggccggacca ctatgcagcg tggaagcgcg
    10741 acgccgagaa ggtgcgcatc ccgggtgggg agagctttgg cgagctgcgc gaccgcctgg
    10801 tgccggcgct gaccgcgata gccgagcgcc acgcgggcga gaatgtcgcc gttgtggcgc
    10861 acaagtcgag caaccgcgtc ttcctctgcg ccgtgcttgg tctttccccc tcgctctacc
    10921 ggcgcatcgg ccaggagaac gcggcggtca acctcatctc ctacgaagga tgccggtggc
    10981 gcgtggatct agtcaacgat acctgtcatc tccggctgcc agggaactcg gggtaacggg
    11041 gattcctccc agcgccgggg caccttcacc gaaggcaaag acacgcatcc ccacgaaccg
    11101 ccggaaggtg ccggtgccct tgatcgcggc aagcctccgc accagcagca ggtcggggtc
    11161 gccatctccc gcgacgccgt gaatgacgaa gatggtgccg cgttgggcgg cgcggcgcgc
    11221 gagggcatcc agctccgccg tgtcggccgg cgtcagcggg gcccggccgc gggggtaccc
    11281 cgttcggggc gggttgccgc ggctatagta atcaaacggc aggtggagga acggcatcgt
    11341 cagaaagacc tcggtgtccg ggttgcgcgc cgagcgcgcc gccgtctgca tcatgcgcac
    11401 cgcttcgcgc cactcctctt tctgccattc ccgtgtcgcc gccttcatcc cgccagcgtg
    11461 tagcagcacc aggatcgtcg cgaggaggac gcgccgggcc ccttgctggc gggtgagaac
    11521 ccacgccagt accaggaggt agccgggcag cgccaccagc agataccgct cttgccagac
    11581 tggcttcatg agggccaggc cgataaagag taccatcggc accagcagat agaggagcga
    11641 gaagaggagc gggcgtccgc catccgtatc ctccctcttt gccgagcgtc ctgccagcca
    11701 cagggggtag accgcggcga tccccaggag cagggcgcac gccgcctgga tctcgggcgg
    11761 gtcgctcgcg ctggtgggct tgccaaagag cttgttcccc atacgcgcca aggcgccagg
    11821 agtcacatgg ccttgccaaa actggtaacc atcgcgcaag aggaaggtcg gcgccaggta
    11881 caacacaacg cccggcaggc agaggaggcc aaagagcgcc gctgcgccga cggcgcgcca
    11941 tcgccttgcg gcgaccaggg cataggcgcc ttgaaacgcg ggaatgagca tcgccgagta
    12001 gtggatgtag aaggccaggg cggcaaagaa cgccgcacct agccaatgcg cccagcggtc
    12061 cgtcttgacg gcgcgcatga agagccaggc cgagactagg ccaaggaaca tcgcgggggt
    12121 atactgccgc gcctcctgcg agtagaagag gtggatgggg gagaacgcga gcaggagagc
    12181 ggcgagcgcg ccggctctcc tgccgaacag ctctcgtccc aagaggtaga tcaccggtac
    12241 tgagaggacc ccggccactg cggagaagct gcgaagggcg atctcagagt cgccaaaaag
    12301 tgccagccac tggcccagcg ccaggtagta gagcggtggg ttccccggat ccgcgttgcg
    12361 cgtcagaagc tccgcgaccg gaagccgggc aaacgaccaa ctcgctgcct cgtccagcca
    12421 caggctctcg acgccaatcc gccagaatcg cagtgccgcg gctaccacga tcagccctgc
    12481 gatgagcgcc cactccaggg acgctcgatc ctctcggttc accgccggat ggttcgatgg
    12541 cttcaacatc ctgtgatgcc tcctgaacga gggcagggca ggatgcgtgc caaggagggc
    12601 gcgatggggg agtagtctcg cgctcgaagt gcccggagat caggggcatg cgggagctta
    12661 cgcctcaagc agggcgatag ccacggcggt ggagtaggtc tcgcagtgcg agatgctgac
    12721 cagcacgcgc cggccggcgg cgatctcgcg cgctttgcca tggagggtgc agaccggctt
    12781 gccacgctcg tcgttcagga tctcgatctc gcgccagggg acggcgcgcc cgagtgcctt
    12841 gatcaccgcc tctttggcgg caaagcggcc ggcgaagtgc tgcgccggcc ggccacgcgc
    12901 gctgcaatag gagacctccg cctcggtgaa gaggcgcttt gagaaggcag ggctgcggtt
    12961 catggccgct tcaatgcgcg cgatctcaat gatgtcggta ccgagtccat cgatcatggg
    13021 ctggagcctc acacgttcac cacgaggtta tcgcggtgga cagcctcctc gaagtcacac
    13081 gcgccgaggt agttggccac ctcgtcggtg tgcaggccgc gtacggcggc gagatcctgg
    13141 gaagcgtagt tggtgaggcc gcgcgccagc tcccgcccct cttcatcttc gatgcggacc
    13201 agatcgcccg cctggaagcg cccctccacc gcgacgatcc cggcggggag gaggctatgc
    13261 ccctcttcca cgagcgagcg acgagcgccc tcgttgatga tgatggcgcc gcgaggcctg
    13321 gccccgaagg cgatccaccg cttgcgcgcg cgcaggcggt gctcgctggg cggaaagacg
    13381 gtgccgaggc gctctccctc cagaagggcg cagatgacgc cgtcgcgccg gccatgcgtg
    13441 atcactgtgg tcgccccgca gcgcgcggcg atgcgcgcgg cctgcagctt tgtgaccatc
    13501 ccgcccgagc cgactgcgct cccggggccc cccgccaggt gtgccacgcg ggcg
//
LOCUS       DAOSCV010000008        13334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3143_length_13334_cov_3.599669, whole genome shotgun sequence.
ACCESSION   DAOSCV010000008 DAOSCV010000000
VERSION     DAOSCV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_13334_cov_3.599669"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PLY56_00445"
     CDS             complement(<1..725)
                     /locus_tag="PLY56_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19979.1"
                     /translation="MPFELEPVPPQPPESEWAFVDELRVTPYHQRVIWRRREPQPGEA
                     EFRDGVRLDLRFSDPGGLLETAFADFRRFLQLGGIREDGPYTLTVHHCAVPCREAFVV
                     RVERDGCFLGAGDTEGIRRGLVWLEDEMLRREGPFLPLGEFTRRPVIRTRVARCFYGP
                     INRPPKSRDELADNVDYYPEEYLNRLAHEGVNALWFTLHFFRTVPSRIIPEYGQNAAP
                     RLAKLRWMVEKCARYGIRIYPFC"
     gene            985..2247
                     /locus_tag="PLY56_00450"
     CDS             985..2247
                     /locus_tag="PLY56_00450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ19980.1"
                     /translation="MLQFDRFGFSPNLMKALAEAGYKTPTPIQAQAIPLALQGDDILG
                     CAQTGTGKTAAFALPILHRFSETGRKPRALILAPTRELASQIEASFAAYGKHLPTRVC
                     ALYGGVPLKGQIARLRKGIDILVATPGRLMDHVERHTVDLSGIEVLVLDEADRMLDMG
                     FEPQVRAIAGALPANRQTMLFSATVPSAIESLVRRYLRTPKRVEVERSGTTVDTIDQR
                     VHLVAPEQKTTLLARVLQDEKPTSTLIFTRTKRGADRLTQTLRSQGFKAEAMHGDLAQ
                     NRREQIIRSFRSQHIHVLVATDVAARGLDIPHISHVINYDVPEEAEAYVHRIGRTARA
                     QRTGVAITLASARDQAALASIERLVGQSIPRHTLPGFDYQHDTSHITSQAARGGRGGG
                     RNASRADAVLAFLERRYGRAARATRAPA"
     gene            complement(2262..4286)
                     /locus_tag="PLY56_00455"
     CDS             complement(2262..4286)
                     /locus_tag="PLY56_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ19981.1"
                     /translation="MIRHSLALLGALLAAMAAPVAFAAPRENLLPNPDFEAEDAAGWE
                     KRTPDDADRALSIVAEAAHSGKRGARITNRKATISRWRQGADGKIKVPEGATIRLSGW
                     IRTDLGPEGYASLRLYCMSDRGEITAQRDSRQVIGKSDWTRVAAGMKVPEGTQYVMAY
                     LELQNAVGTADYDDLSLEVLFVPEPVQVRRDLILLTDAPEEDATVRNLETLYPRRITR
                     SAPGAQADWESAQGAIVYLRDPAKAPDLAAVERFAASGKPVVMDLGVYARARGLELKE
                     TTGEDEPTLRIVEEDPLTRGFRAGDTIPWYGGARGAYTQRTLATPGRARVLAESSRGG
                     ALLVVEKVGQGSVLATDLITLPEPVWSRPGSFNKYLFLGNLLGKSARYGRYFRERLTY
                     EGFVAAMRALAAENPALRFKEEGPAYGDYRLYSLNLGDPSRPAFLVYGVAHGSEWEPG
                     YGLLELARLLASPEGKGLFDTSRYSLKIVPIINPSGYDLFIRHNANKVDLNRNAIDHW
                     ETYQGRDSNKDGVYGPGDSDWKGTASCSEPETRTLRRICEELKPYATLDFHGNAGGRG
                     NNRLILLPRYARPENEELAYLVAEEFNQAFRDRYVLHEASRPGVQQYEIEAVQWSPPR
                     PTLTAMATRDRLGFVCEVPAGYRSTYGTVFQTDVVIETALAFFRVYQKQP"
     gene            4673..5578
                     /locus_tag="PLY56_00460"
     CDS             4673..5578
                     /locus_tag="PLY56_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HOJ19982.1"
                     /translation="MEIPEILEELSRYTGKLPREAIREAVARQEEITPELIRVIDEAR
                     ETLARGETLEESTALTFALYLLAQFRETRAYPAIAELLSIPGDAIADTLEEVLTEDMG
                     RILASVSGGDTSHIERLIENPNVNEYVRSAGLKALVTLVMTGMKERDEVISYFRSLFH
                     GRLEREGGFVWAKLVVVSTDLWPGELMDEIRQAYEEALVERFVISLKEVERQLSQGKE
                     AALELTCRIGNYTLIDDTEAELGSWYCYNEAPPPKPPVAAWKPPSPAQAPATPGMPVR
                     KGRKIGRNEPCPCGSGKKYKRCCGA"
     gene            6058..7467
                     /locus_tag="PLY56_00465"
     CDS             6058..7467
                     /locus_tag="PLY56_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19983.1"
                     /translation="MTSLRRSLLALFWCCLFVAMAAKRPACASLALPEPAVAVVATIG
                     SQGGGQDLVPQNAEVVLHERGLDVTQWRGIVMGAAPTTTSLRETLAAALPPDERRLVF
                     HPRVGRHTVPEEMPVLLEAVRVLCRGEKDERAARAVHQALCWIVGNQLDEALWQLAEA
                     RSGHGQWRLEPPRPPRPGDREDPCKVHLALLFERPPADGEEGSQLGPFHLIFHGPEFD
                     TIARGMAEQRAALGREEFTRRVVQATRKQQPPFLDDARASTATRLALRGVPIAEAAKR
                     IAAEASLPLEIASVPAQPLVTVVSEGATAGDLLLALARIGRLTLDHVDEGYLLTPPRT
                     TAERLWSALPLPLWGLARATPTERQIAREATIRELWPLLPGATLGTLRSLPEAARARV
                     ARLVEIGFAAAFRMQMADLPDATGTAHLALYESETTGNFMLGTPGASETIGGAAYLQL
                     KTELTGLPWIAMPAKGEGK"
     gene            7464..8978
                     /locus_tag="PLY56_00470"
     CDS             7464..8978
                     /locus_tag="PLY56_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19984.1"
                     /translation="MTRLLAALGALVLSAVAAGAAPTGPPYRVDLTGRTLEEVAVTLH
                     RDFQIKTAIARPAPTRGKKTVQADTLWEMVDAILEEYGVDGFLFGDLLAIGEKPALQA
                     PSTTPDPGEAGRTVRLLLVELWERVKPEALPDGIECVVIPAPLGEREIAAVRDYYRAE
                     VARGEIPARSSPKLASEEKSWLRVVDGMLVVETESAHPKPARRPLGPVPPEAAGEPSA
                     WQNLLSHLGSGQPFAKTRLAATVNLEGKGTLGQLVAQIARAVVADIAVDPTLAELPVA
                     VTAPGVSAAEALDLLAVATGAQWGPPHDRIRYTMDPLPSRGLRARERWAMLKGSRMGS
                     PSWARHVWPHLTPKQRAALQRGEKVWIPSIGKSARRWIDFTVAVYAGGGLSSAHSAMK
                     RAETGSMHATFAAYPDGLVLGRVHSSVSGSTTSLCWPMTDKKLERTGTPDDFGPTIGG
                     GAERFGSYDNSYFTDRYSLFHSHAVRSRGYGGRVLRGSWGPPPPWSEKEGEIKR"
     gene            8975..9523
                     /locus_tag="PLY56_00475"
     CDS             8975..9523
                     /locus_tag="PLY56_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19985.1"
                     /translation="MKYLTSLFLGCLLGLGTPSNAVAEVTAEGWAEHRAVPAGIYYPG
                     DTVEAWAYANGRVHIQAFGRNDTWNYWNCVGAYASDGIGEVPGPYPYQALANGQGPVD
                     LVCDIPDIWLDMTTLDWEHLGTHQSVALSEVHVSVRVNGDGPADVDYPLRKEETTYEV
                     RAIEPPGGDEELPVVVGPDEIS"
     gene            complement(9641..10531)
                     /locus_tag="PLY56_00480"
     CDS             complement(9641..10531)
                     /locus_tag="PLY56_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ19986.1"
                     /translation="MFIDIHVHTRYFRGPERLVGGTYATPEELIEMLKPRGVRKAVIL
                     PGVNPECAFIIQSMGEVLAIVEKYPDFFIPFMNIDPRQVRNSPDADLGYLMRYYQERG
                     CKGIGEICASLPFDHPLMENLFRHAEACGLPVLFHIAPKQGGYYGIVDSLGLPGLEGA
                     LRKFPKLTFIGHSQPFWAEISGDLTEDQRNGYPKGKVAEGGAVVRLFREYPNLWGDLS
                     PAAGSGFNAISRDPEFGYAFMEEFQDRLLFGTDICDPRNKLTLIDYLNTAVAEGRLSR
                     EAYEKLGWKNAERLLGLPSA"
     gene            10748..11857
                     /locus_tag="PLY56_00485"
     CDS             10748..11857
                     /locus_tag="PLY56_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19987.1"
                     /translation="MKKVLLMLALLTATATAFAAENQAFWGVFAETRVQKMAGMPALP
                     PLPPGVDAGAMAQIPGMAAVLGMGAPQRTLSVRLWSPGIAPQGATATITPPAGLKQGD
                     RLVLDLYRPKPGETGPGEEAGTEESSFTIKRYWGSSEKVKPGQPEVIRWDTLSPELKA
                     AARREAAEAASKESYFYKPDWTTAYWPTGKQPGRIANDASLPGKYTLTTSYTGSVSID
                     VPQGVDFLAPIELSSPDLTKLPSLDGAITFRWKPVPGILGYHARIIGMQQEKKTLILW
                     SSSEVQDEPSGGWDYLEMAEVAKLVKSTAMMEPTRQEMTVPAGIFKECDVVYFTMTGY
                     GPGAALAEGQPLPRVQTKTTLNIMLGGKAMGQMGE"
     gene            12255..13292
                     /locus_tag="PLY56_00490"
     CDS             12255..13292
                     /locus_tag="PLY56_00490"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ19988.1"
                     /translation="MSQFTVAVVGVGAVGEEMLRVLAQRNFPAREIRALARSARTITV
                     DGRSYDVQETTPEAFEGVDIALFAGTEGESGAAVTFAAEAVRRGAIVIDNGADFRMKP
                     EVPLVVPEVNADAIEKHQGIISNPNCSTIQMVVALKPLHDAGKLKRVIVSTYQAVSGT
                     GRAAIRELEAQVKAWAGGEELPAPSAYKYPIAFNLIPQIGSFRPDYYTSEEWKMIAET
                     HKILGDDSIAISATTIRVPVFNSHSEAVTVETEEKITREEALRLLAAAPGIVVVDDPS
                     VAGEGGPAYPMPIETTGKDLTYVGRVREDPFVPNTLHMWVVADNIRKGAATNAVQIAE
                     ALVERGLVGKK"
BASE COUNT         2325 a         4440 c         4372 g         2197 t
ORIGIN      
        1 atgcagaagg ggtagatccg gatgccatag cgcgcgcact tctccaccat ccaccgcagc
       61 ttcgccaggc gcggcgccgc gttttgcccg tattccggga tgatccgcga gggcaccgtc
      121 cggaagaagt ggagtgtgaa ccagagcgcg ttcaccccct cgtgcgccag ccggttcaga
      181 tactcctccg ggtagtaatc cacgttatcg gccagctcgt ctctcgattt gggagggcgg
      241 ttgatcggcc cgtagaagca gcgcgcgacg cgcgtccgga ttaccggcct tcgggtgaac
      301 tcgcccaggg gaaggaatgg accctcgcgc cggagcatct cgtcttccag ccagaccagc
      361 ccgcgccgga tcccctcggt gtcgcccgcc cccaggaagc agccgtcgcg ctccacgcgg
      421 accacgaacg cctcgcggca tggcacggcg cagtggtgca cggtaagggt gtagggtcca
      481 tcttcgcgga tcccgcccag ctggaggaag cggcggaaat cggcgaaagc cgtctcgagc
      541 aagccgcctg ggtccgagaa gcggagatcc aggcggacgc catcgcgaaa ctccgcctca
      601 cccggctggg gctcgcgccg gcgccagatc acccgctggt ggtagggagt gacgcgcaac
      661 tcatcgacaa acgcccactc ggactccggc ggctgcgggg gaacgggttc gagttcaaac
      721 ggcatgggtc ggctctcctc acgcggccag cctccgcgct ccggacgcga caccccggcc
      781 atccagacgg cgaggtgcgc ccggatcacg gcttgcagaa taccgcgatc tctgttatac
      841 tgttccgcag aacagtcgcg attcccctca ttggtccggc gcgagtccca tcctgtgcct
      901 ctcgcttacc ccgcggcgcc tgttccattg cgttgcgcgt gtcggtgggc gggggactgc
      961 acacgccggt actaaagagg tgatatgctt cagttcgatc ggttcgggtt ctccccgaac
     1021 ctgatgaaag ccctggcgga agcgggctac aagacgccca ctccgatcca ggcgcaggcg
     1081 attcccctgg cacttcaggg ggatgatatt ctcggatgtg cccagacggg cacgggcaag
     1141 acagccgcgt tcgccctccc tatccttcac cgcttctccg aaacaggcag aaagccacgc
     1201 gcgctcatcc tggcgccaac gcgcgagctc gcttcccaga tcgaagccag ctttgcggcc
     1261 tacgggaagc acctgcccac gcgggtgtgc gcgctctatg gaggcgtgcc gctcaagggg
     1321 cagatcgccc gcctgcgcaa aggcatcgat atcctcgttg ccactcccgg ccgcctcatg
     1381 gaccacgtcg agcggcacac cgtcgatctc tccggcatcg aggtgttggt gctggatgag
     1441 gccgaccgca tgctcgacat ggggtttgag ccccaggtgc gcgccatcgc cggcgccctg
     1501 cccgccaacc ggcagaccat gctcttctcc gcgaccgtac cctcggccat cgaaagcctg
     1561 gtacgccgct atctgcgcac gcccaagagg gtcgaagtgg agcgcagcgg caccaccgtc
     1621 gacaccatcg accagcgcgt gcacctggtc gcgccagagc agaaaacgac gttgctggcg
     1681 cgtgttctcc aggatgagaa accgacaagc acgctcatct tcacgcgcac caagcggggc
     1741 gccgaccggc tcactcaaac cctccgctcg caaggcttca aggccgaggc gatgcatggt
     1801 gacctggcgc agaaccggcg cgagcaaatc atccgttcgt tccgctccca gcacatccac
     1861 gtactggtag cgacggacgt ggccgcgcgc gggctggata tcccgcacat cagccatgtg
     1921 atcaactacg acgtgcccga ggaggccgag gcatacgtgc atcgcatcgg gcgcaccgct
     1981 cgcgcgcagc gtaccggagt cgcgatcact ttggccagcg ccagagacca ggcggcactg
     2041 gccagcatcg agcgcctcgt tggccagagc atcccgcggc acaccctccc gggcttcgat
     2101 taccagcacg atacctcgca catcaccagc caagcggcgc ggggcggtcg cggtgggggg
     2161 cgcaacgcca gccgcgccga tgcggtgctc gccttcctgg agcgccgcta cggccgcgcg
     2221 gcgcgcgcca cgcgtgcacc cgcatgagcg aggcgatctg actacggctg tttctggtaa
     2281 acccggaaaa aggcaagcgc ggtctcgatc acgacatcgg tctggaaaac ggtgccgtag
     2341 gtgctgcgat agccagcagg cacctcgcag acgaagccga ggcggtcgcg tgtcgccatc
     2401 gcggtgagtg tgggccgggg ggggctccac tgcactgcct caatctcgta ctgctgcacg
     2461 ccggggcggc ttgcctcgtg aagcacgtag cggtcgcgga acgcctggtt gaactcctcc
     2521 gccaccaggt acgcgagctc ctcgttctcc gggcgcgcgt accgggggag caggatcagc
     2581 cggttgttgc cccggccccc ggcgttgcca tgaaaatcga gcgtcgcgta gggcttcagc
     2641 tcctcgcaga tccggcgcag ggtgcgcgtc tccggctcag agcacgacgc cgtccctttc
     2701 cagtccgagt cgccggggcc gtacaccccg tccttgttgc tatctcgtcc ctggtaggtc
     2761 tcccagtggt cgatggcgtt gcggttcagg tcgaccttgt ttgcgttgtg gcggatgaag
     2821 aggtcatacc cggaggggtt gatgatcggc acgatcttca gcgagtagcg gctggtatcg
     2881 aagaggccct tcccctcggg cgaggcgagc agccgggcga gctccaggag gccatacccg
     2941 ggctcccact cgctcccgtg cgcgacgcca tacaccagga aggcgggccg gctcgggtcg
     3001 cccaggttga gcgaatagag ccggtaatcg ccatacgccg ggccctcttc cttgaagcgg
     3061 agcgccggat tctcggcggc gagggcgcgc atggcggcga caaacccctc gtaggtcagg
     3121 cgctcgcgga agtaacgccc gtagcgcgcg ctcttgccca acagattacc gaggaagagg
     3181 tacttgttga agctgccagg ccggctccaa accggctccg ggagcgtgat caggtcggtg
     3241 gcgagcacgc tcccctgccc caccttctcc acgacgagga gggcgccgcc gcgcgacgat
     3301 tcggccagga cgcgcgcccg gccaggcgta gcgagcgtgc gctgggtgta ggcgcctctc
     3361 gcgccgccat accaggggat cgtgtcgccc gcgcggaagc cccgggtgag cgggtcctct
     3421 tccacaatgc gcagggtcgg ctcgtcctcg ccggttgtct ctttcagctc caggccgcgc
     3481 gcccgcgcgt aaacgccgag gtccatgacc accggcttgc cgctcgcggc gaagcgctcc
     3541 acggccgcca ggtccggggc cttcgccggg tcgcgcaagt agacgatggc accctgggcg
     3601 ctctcccaat ccgcctgggc tcctggcgcg ctgcgcgtga tgcggcgcgg gtagagcgtc
     3661 tccaggttcc gcacggtggc gtcctcctcg ggtgcatcgg taaggaggat caggtcgcgc
     3721 cgcacctgca ccggctcggg cacgaacagg acttccagcg agaggtcatc gtagtcggcg
     3781 gtgcccacgg cgttttgcag ctccaggtag gccatcacat attgggtgcc ctcgggcacc
     3841 ttcatcccgg cggcgacgcg cgtccaatcg gatttgccga tgacctgccg ggaatcgcgc
     3901 tgcgcggtga tttcgccacg atcgctcatg cagtagaggc gcaggctggc gtagccctcg
     3961 ggccccaggt cggtgcggat ccagccgctg aggcggatgg tggcgccttc cggcactttg
     4021 atcttgccat cggcgccctg gcgccagcgg ctgatcgtgg ccttccggtt cgtgatccgc
     4081 gcgcctcgct tgccggagtg ggccgcttcc gcgacgatgc tgagtgcgcg gtcggcgtca
     4141 tccggggttc gcttttccca tccggcggcg tcttccgcct cgaaatccgg gttcgggagg
     4201 aggttctccc ggggcgccgc gaaagcaacc ggcgccgcca tcgccgcgag aagagctcca
     4261 agaagcgcga gagagtgacg gatcatgacg ctcacctgcg cgtcggcccg tgcggaccgg
     4321 cgccccttcc aagcgattgc cgttttcccc acagttctgc gcgcgcccgc tttctcctcg
     4381 gtaggcgtgc cggatgggcg aggaggagcc aggccaatgg cgaaccacgg gcgtatggtg
     4441 ttcaacaacc gggcgtatga gcaccgctcg cctggtaagg cggtatctca tggaggttct
     4501 gcgatgaggt acttcctgat cttgtgtctg ctcgtttctg ccggaggagt cggcgcgcag
     4561 accaaagtcg agtacgagag tgagcaatcg aatggacgct cgaatagcct ccctacgagt
     4621 ggcgaggtag catcgccctg gcccagggcc gtagaacgaa aggcgaaggc agatggagat
     4681 tcctgagatt ctggaggaac tgtcgcgata tacgggcaag ctgccccggg aagcgatccg
     4741 ggaggcggtg gcccgccagg aggagatcac gccggaactg atccgggtga tcgacgaagc
     4801 gcgggaaacc ctcgccaggg gcgagaccct agaggaatcg actgccctca cattcgctct
     4861 ctacctcctc gctcagttcc gtgagacgcg cgcctacccg gcgattgcgg aactcctctc
     4921 catccccggc gatgcgatcg cggacacgct ggaagaagtg ctgacggagg acatggggcg
     4981 catcctggcc tccgtttccg gcggcgacac gagccacatc gagcgcctga ttgagaaccc
     5041 gaacgtcaac gagtatgtac gtagtgccgg cctcaaggcg cttgtcactc tggtcatgac
     5101 cggcatgaag gagcgggacg aggtcatctc ctacttccgg agcctgttcc acgggcgcct
     5161 ggaaagagag gggggctttg tctgggccaa actcgttgtc gtctccaccg atctctggcc
     5221 aggcgagctg atggatgaga ttcgccaggc gtacgaggag gctctggtcg aacgctttgt
     5281 tatcagcctg aaggaggtcg agaggcagct ttcccaaggc aaggaagccg cgctggagct
     5341 cacctgcagg attggcaact acacgctgat cgacgatacc gaggccgagt tggggtcgtg
     5401 gtactgctac aatgaggctc cgcctccgaa gccaccggtt gcggcctgga aaccaccctc
     5461 accggcacag gcacccgcga cacctggcat gccggtgcga aagggtagga agatcgggcg
     5521 caacgagcca tgcccctgtg gcagcggcaa gaagtacaag aggtgttgcg gcgcctagcc
     5581 acgccagcgg atcccacccc cgtccggccc ggcggggctt ccactctcgg atagccgcgg
     5641 ccggcggggg ctggaaccga aagcccaaag ggctttttca gataggagcg cgaagcttca
     5701 tctacgcccc cttattgtcc cgcgctggtg cataccgggc gcgcccgcac tctccccatt
     5761 cgcgcccatc cccggcatca acccggaccc ggatagagat ggcaaaaaga ggtctggtgc
     5821 ctcgtctatt aggataggag cttcccccgc ggcctcaccc tgggcgcggg aagcaaccct
     5881 ggcgaggaat cgctgagatt ccgagataag ggaaccgaag cctcggtggt agcgcgcggc
     5941 cctcttcatc cgcggtatcg aaggggacgc gccggcggga gaacacaggc cactatatga
     6001 acgtaccctc atctcggaat cccaggggat tcggatgtgt atcaggaggg catgccaatg
     6061 acttctctac ggcgctcact cctcgcgctc ttctggtgtt gcctctttgt cgcgatggca
     6121 gcgaagagac cagcatgcgc gagcctcgcg cttcccgagc ccgcggtcgc cgtcgtggcc
     6181 accatcggct cgcagggcgg cgggcaagac ctggtgcctc agaacgccga ggtggtgctg
     6241 cacgaaagag gcctggacgt gacccaatgg cggggcatcg tcatgggagc cgccccgacg
     6301 accaccagcc tgcgcgagac actcgccgcg gcgctcccgc cggacgagcg ccggctggtg
     6361 ttccacccgc gggtgggccg ccacacagtc cctgaagaga tgcccgtgct gttggaggcc
     6421 gtgcgcgtcc tctgccgggg cgagaaggac gagcgcgccg cgcgagcggt ccaccaggcg
     6481 ctctgctgga tcgtgggcaa ccagctggac gaagccctgt ggcaacttgc ggaggcgcgc
     6541 tccgggcatg gccagtggcg tctcgaacct ccgaggcccc ccaggcccgg cgatagagag
     6601 gacccgtgca aggtccatct ggctctcctg ttcgagcgcc ctccggcaga tggagaggag
     6661 gggagccagc ttgggccgtt ccatctcatc ttccacggtc ccgaattcga tacgatcgcc
     6721 cggggaatgg cagagcagcg cgccgcgctg gggcgcgagg agttcacgcg ccgggtggtg
     6781 caggctacgc ggaagcagca gccgccgttt ctcgatgacg cgcgagccag cacagccaca
     6841 cggctggcct tgcgaggcgt tccaatcgcg gaagcggcga agcggattgc cgcggaagcg
     6901 agcctgccac tggagattgc gtccgttcca gcacagcccc tggtcacggt ggtcagcgag
     6961 ggcgcaaccg cgggagacct gctgctggcg ctggcgcgca tcggccggct caccctggat
     7021 cacgtggacg agggctacct tctgacgcca ccgcgcacga cagccgagcg gctctggagc
     7081 gcactcccct tgccgctctg ggggctggcg cgcgccactc ccactgaacg ccagatagcg
     7141 cgtgaagcca ccattcgcga gctctggcct ctcctccccg gcgcaacgtt gggcacgctc
     7201 cgatcgctgc cagaagctgc gcgtgcccgc gtggctcgcc tcgtggagat cgggtttgcc
     7261 gccgcgtttc gcatgcagat ggccgacctg cccgatgcca cgggcacggc gcatctcgcg
     7321 ctctatgaga gtgagaccac ggggaatttc atgctgggca cgcctggggc ctcggagacc
     7381 atcggcggtg ccgcctatct ccagctcaag accgagttga caggcctgcc ctggattgcc
     7441 atgccagcga agggagaagg gaaatgacca gacttctggc ggctctggga gcgctcgttc
     7501 tctctgccgt ggcggcaggc gccgcgccca ccgggccccc ctaccgcgtg gacctcaccg
     7561 ggcgcaccct cgaagaggtc gccgtcactc ttcaccgcga cttccagatc aaaacggcca
     7621 ttgcgcgccc ggccccaacc cgaggcaaga agactgtgca ggccgacaca ctctgggaga
     7681 tggtggatgc catcctggag gagtatggcg tcgacggctt ccttttcggc gacctgctcg
     7741 ccatcggcga gaagcccgcg cttcaggcgc cctccaccac gcccgaccca ggagaggctg
     7801 gtcgcacagt ccgccttctc ctcgtcgagc tttgggagcg ggtgaagccg gaggcgcttc
     7861 ccgatggcat cgaatgcgtc gtcatcccag cgccgcttgg cgagcgagag atcgcagcgg
     7921 ttcgcgacta ctaccgcgct gaagtggcac gcggcgagat acccgcccgc tcctctccga
     7981 agttggcgag cgaggagaaa agctggctcc gtgtcgtgga tgggatgctg gtcgtggaga
     8041 cggagagcgc gcatcccaag cccgcccggc ggccgctggg ccccgtcccg cctgaagccg
     8101 cgggggagcc ctccgcctgg cagaacctgc tctcccacct gggaagcggc cagcctttcg
     8161 ccaagacacg cctggcagca accgtcaatc tggaagggaa gggcacgctg ggccagctgg
     8221 tcgcccaaat cgcgcgcgcc gtggtcgctg atatcgcggt ggaccccacc ctcgcggaac
     8281 ttccggtggc ggtgacggct cccggcgtct cggcggccga ggcgctggac ctcctcgctg
     8341 ttgccaccgg ggcgcagtgg ggcccccctc atgaccggat tcgctacacc atggatcctc
     8401 tcccatcgcg cggcctccgc gcccgagagc gatgggcaat gctgaagggc agccggatgg
     8461 gaagcccatc ctgggcgagg cacgtctggc cgcacctcac acccaagcag cgtgctgccc
     8521 tgcagcgggg cgagaaggta tggatcccct ccatcggaaa gagcgcgcgc cggtggatcg
     8581 atttcaccgt cgcggtctat gccggcggtg gacttagctc cgctcacagt gctatgaaac
     8641 gagcggaaac gggctccatg cacgcgacct tcgcggccta tcccgacggc ctggtcctcg
     8701 gccgggtcca ttccagcgtt tccggctcaa ccacatcgtt gtgctggcca atgacagaca
     8761 agaaactcga gagaactggc acgcccgacg atttcggacc caccatagga ggcggagccg
     8821 agcgttttgg ctcgtacgac aactcctatt tcacggatcg ctactccctg ttccatagcc
     8881 acgcggtgcg ctctcgcggc tatggcggga gagtgcttcg cggctcctgg ggcccgccac
     8941 ccccatggag cgaaaaggaa ggagagatca agcgatgaag tatctcacat ccctctttct
     9001 aggctgcctg cttggcctgg gcaccccatc caacgcggtt gcggaagtga cggccgaggg
     9061 atgggccgaa catcgcgccg tgcccgccgg gatctactac ccgggtgata ccgtcgaagc
     9121 ctgggcctat gcgaacggca gagtccacat ccaggccttc gggcgaaacg acacctggaa
     9181 ctactggaac tgcgtgggcg cttacgcgag tgacggcatc ggcgaggtgc caggccccta
     9241 cccgtaccaa gccctggcga atggccaggg tccggtggat ctcgtctgtg acattccaga
     9301 catctggtta gacatgacga ccctggactg ggagcatctc ggcacgcatc aatcggtcgc
     9361 actcagcgag gtgcatgtct ccgtccgggt caacggcgac ggcccggcgg acgtcgacta
     9421 tcccctccgc aaagaggaga ccacgtacga ggtgcgggcc atcgagccgc ctggcggcga
     9481 tgaggagctc cccgtcgtcg tcggtcctga cgagatctcc taacactccc atagccactg
     9541 cttgtggggc tctcccccct cagctggtgg ctactggtgt aaagctctgg cagggagttg
     9601 cgatgggccg gccgtttcgc tccggccggc ccgatttccc tcacgcggaa ggaagcccga
     9661 gcaggcgctc ggcgttcttc cacccgagct tctcatacgc ttcccggctc aggcgcccct
     9721 cggcgacggc ggtgttcaga tagtcgatga gcgtcagctt gttccgcggg tcgcagatat
     9781 cggtgccgaa gaggagccgg tcctggaact cctccataaa ggcgtagccg aactccgggt
     9841 cgcgcgagat cgcgttgaac ccgctcccgg cggcgggcga gaggtcgccc cacaagttgg
     9901 ggtattcgcg gaagagccgg acgacggcgc cgccctcggc caccttgccc ttgggatagc
     9961 cgttgcgctg gtcctccgtc aggtcgccac tgatctcggc ccagaatggc tgcgagtggc
    10021 cgatgaaagt gagcttcggg aacttgcgca gggcgccctc caatccgggc aggcctaggc
    10081 tgtccacgat gccgtagtag ccgccttgct tcggcgcgat gtggaagagg accggcagcc
    10141 cgcacgcctc ggcgtggcgg aagaggttct ccatcagcgg gtggtcgaac gggagactgg
    10201 cgcagatctc tccgatcccc ttgcacccgc gctcctggta gtagcgcatc aggtagccga
    10261 ggtcggcgtc gggcgagttg cgcacctggc gcgggtcgat attcatgaag gggatgaaga
    10321 agtcggggta cttctcgaca atcgccagaa cctcgcccat gctctggata atgaaggcgc
    10381 actcggggtt cacacctggc aggatgaccg ctttgcgcac tccgcgcggc ttcagcatct
    10441 cgatcagctc ttctggggtg gcataggtgc cccccacgag gcgctctgga cctcggaagt
    10501 agcgcgtgtg aacgtgaatg tcgatgaaca tcagggagcc ctccttggcg ttcacttcgc
    10561 gccgccaggc ggctttcctc cctggcgccc ggcccatctc agcgccagat ctcctctgcc
    10621 ggtgccgggc gccccagcca cggagggctt gacggatggg cgccagacgc gcgataatgc
    10681 tcccaggcgt ccggctcccg gtgagttggg ggaagggcca ccgggcgtcg cttcggaagg
    10741 agatcgcatg aagaaggttc tgctcatgct cgcactcctg accgccacgg ctaccgcctt
    10801 cgcggcggag aatcaggcgt tctggggtgt tttcgccgag acgcgtgttc agaagatggc
    10861 tgggatgcct gcgctcccgc cgctgccgcc cggcgtggac gctggcgcca tggcccagat
    10921 ccctgggatg gcagccgtgc ttgggatggg cgccccccaa cgcaccctca gcgttcgcct
    10981 ctggtcgccc ggaatcgccc ctcaaggcgc cacggccacc atcaccccgc ctgccgggct
    11041 caagcagggc gaccggctcg tccttgatct ctaccgcccg aagccgggcg aaacggggcc
    11101 cggcgaggaa gccggcaccg aggagtctag cttcaccatc aagcgctact ggggctcctc
    11161 ggagaaggtg aagcccggcc agcccgaggt gatccgttgg gatacgctga gcccggagct
    11221 gaaagcagcg gcacggcgcg aggcagccga ggcagcgagc aaggagagct atttctacaa
    11281 gccggattgg acaaccgcct actggcccac tgggaagcag cccggcagga tcgcgaacga
    11341 tgcctcgctc cccgggaaat acacccttac gaccagctac acgggaagcg tctccatcga
    11401 tgtgccgcag ggggtcgatt tcctcgcgcc catcgagctc tccagcccgg atctcacgaa
    11461 gctgccgtcg ctggacggtg cgatcacctt ccgctggaag ccggtgccgg gcatcctcgg
    11521 ctaccacgcg cgcatcatcg gaatgcagca ggagaagaag actctcatcc tctggtcctc
    11581 ttccgaagtg caggacgagc ccagcggcgg atgggattac ctggagatgg cggaggtggc
    11641 gaagctcgtg aagagcaccg ccatgatgga gcccacgcgc caggagatga ccgtgcccgc
    11701 cgggatcttc aaggagtgcg acgtagtgta cttcaccatg accggctacg gccctggcgc
    11761 cgccctcgcc gaaggccagc cactcccgcg cgtgcagacg aagaccacgc tcaacatcat
    11821 gctcggtggg aaagccatgg gccaaatggg cgagtaggcc ccgcaaggtg ttggcaacgc
    11881 ggggcgaagc ccaatacgga ctcgccccgc gccccgcaac tgtttccccc actcactgca
    11941 gcgccgcgcg aaaggcctac ttccccgccc cgaggagggc gcccccccgg ggggcgcttg
    12001 tcgacgctct catttccgtc tgccgcgacg attccgggcc ggcctctcgc gccgaaagag
    12061 ccccggagga tgccgctcgt cagatcgcga tccagcgggt taagtctggt cgccacgggg
    12121 tatcggtgcc aaatgggaga tccacccgga ttcgccttga catgagccgg ttgcgattgt
    12181 tatactggcc ttactggagc acgcatcagg ctccagagat cgattgcatc atgtagagac
    12241 agaggagtgc gaaattgagt cagtttaccg ttgccgtcgt tggagtgggc gcggtgggag
    12301 aggagatgct gcgcgtcctc gcacagcgta acttccccgc ccgcgagatc cgagcgctcg
    12361 cccgctctgc tcgtaccatt accgtagatg ggcgttccta tgacgtccaa gagacgaccc
    12421 cagaggcctt cgagggagtg gacatcgctc tcttcgccgg caccgagggg gaatcgggtg
    12481 cagccgtgac cttcgcggcc gaggccgtgc gccgcggcgc catcgtcatc gataacggcg
    12541 ccgacttccg gatgaagccg gaagtccccc tggtggtgcc cgaagtcaac gcggatgcca
    12601 tcgagaaaca ccaggggatc atctcgaatc cgaactgttc caccattcag atggtggtcg
    12661 cactcaaacc tctgcatgat gcgggcaaac tcaagcgcgt gatcgtctcc acctaccagg
    12721 cggtttctgg cactggccgg gccgccattc gggagctcga agcacaggtc aaggcgtggg
    12781 caggcggcga ggagttgccg gccccctcag cctacaagta tccgattgcg ttcaacctga
    12841 tcccgcaaat cggcagcttc cgccccgatt actacacctc ggaagagtgg aagatgatcg
    12901 ccgagacgca taagatcttg ggcgatgata gcatcgcgat cagcgccacc accatccggg
    12961 tgcctgtctt caacagccac tcggaggcgg tgaccgtcga gaccgaggag aagatcactc
    13021 gcgaggaggc cctccggctc ctcgccgccg cgcccgggat tgtcgtggtg gatgacccct
    13081 cggtggcagg cgaaggcggc cccgcttacc cgatgcccat cgagacaaca ggcaaggatc
    13141 tgacctacgt cggacgtgtg cgcgaggatc cctttgttcc gaacacgctc catatgtggg
    13201 tcgtggcgga taacatccgc aagggagccg caaccaacgc cgtgcagatc gccgaggcac
    13261 tcgtcgagcg tggcctggtc ggcaagaagt aagagaactg gcaacgaacc gctgcgcgct
    13321 cgcgcggcga tgtg
//
LOCUS       DAOSCV010000009        13318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3148_length_13318_cov_3.158184, whole genome shotgun sequence.
ACCESSION   DAOSCV010000009 DAOSCV010000000
VERSION     DAOSCV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3148_length_13318_cov_3.158184"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLY56_00495"
     CDS             complement(<1..280)
                     /locus_tag="PLY56_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ19989.1"
                     /translation="MAKQSYFQSRYLAHKYWGRRPWYVVRGLIQENTNEGDLVLDSFC
                     GSGTTVIEALATGRRAIGVDLNPMACFITRVTAAYADVEAFSDAFERLR"
     gene            complement(311..2179)
                     /locus_tag="PLY56_00500"
     CDS             complement(311..2179)
                     /locus_tag="PLY56_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ19990.1"
                     /translation="MSSPGGQDGPVSGFGESAHASGPGKEAKARRLGQVFTPDPVADL
                     MASLCIDDPDAEVFDPCIGEGALLLAAGRRLAALGNAEWHRQVWGLEVDPEALAVGRR
                     RVAEAAGVEPEAIRVTRGDFLDLPPPSLGMPEGRLLDLELGGPAGESALCDVALMNPP
                     YTRQERLALETKARGGALSRRAGLHAHFWMHLARFVRPGGWLGAITPSTWLSAEYGRE
                     LRAFLLKHFRIHFVIGFERDVFPDATVEACVTILEKRAGCTDAGAPPPVRFLSLSAGA
                     EWLAAASEAAGRECDGEGPGYRLRVVPQAELVAEEGWNRWFLHPSTARAVRAIRSASA
                     GMVPLEAAARIRRGLTTGNNRVFFLDAAQAARQGIEERYLRRVVRSPREIIGLDTESV
                     AQPGLVLFPPGPDEGGSRAAEYLREQGVVTSAYLPTTPPEPAPLLFGYAVRARKAFFR
                     NSAGLLASDNFFEVIPRRPEETSVLFALLNSVVAALHLEAHGRGQGRGLLKIQRYELA
                     GLQLPDPARMDPVARRELEALGEELRQHPEEEGIRAALDYLVARLLGLDAVELRRAER
                     ELASARRVKSLSRGAVVKGREVEWTASPARPGQAERDAFHPAGEAIWGSGALRGAR"
     gene            2355..2675
                     /locus_tag="PLY56_00505"
     CDS             2355..2675
                     /locus_tag="PLY56_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19991.1"
                     /translation="MEWLALLITIILLAIVAFIVVGAAVKILWALLIGAIVGLIANGI
                     VSLIQPSGGPRGCLETALAGVGGSLVATIFFGREGLIPAVLGAILVVIAWKAVTFSRH
                     MESR"
     gene            complement(2740..3999)
                     /locus_tag="PLY56_00510"
     CDS             complement(2740..3999)
                     /locus_tag="PLY56_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ19992.1"
                     /translation="MSDAVARRLVLRASRLIDGTGAAPTDDPVLVVADGRVEAVCQGE
                     LPPALRDGAVFLDLPGQTLLPGLIDSHVHLVFDAGPDHAAVRRRLAEEDDQDLLLRAL
                     HNAQTLLRAGITTARDCGDRGFVTLKVRRAIEDGLFSGPRLLCSGPPITTTAGHLHYC
                     GLHADSLDDVRRAVRGVVERGADYVKIVATGGMMTPGSNPRRCQFSPEELKVAASEAH
                     RLGKRVAAHLLGVEGIQPCLQAGIDTFEHCSWMARDEAEEFDFRPELAREIARSGITC
                     GHTMVGLYRDLLPDREAPEAVQVEQLEQLRALQERFARMRELGIRMMLSSDAGVAGTR
                     FDRFVDGLEVAVVAMGMSPLAAIEASTRVPAEALGLGDEIGTLVPGKRADIVAVKGDA
                     SADITALKRVSLVMRDGRVLHSASTAA"
     gene            complement(4102..4722)
                     /gene="lexA"
                     /locus_tag="PLY56_00515"
     CDS             complement(4102..4722)
                     /gene="lexA"
                     /locus_tag="PLY56_00515"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ19993.1"
                     /translation="MSRQGETRARVFEFLCGFTRQHHRSPTVREIGRALGLRSSETVH
                     RHLVELEREGKIRRISTPNSFVIEILDAEELWLAQPASVAVPLVGRVAAGAPLLAAEH
                     REAVYALPKEWVGEDPTFMLRVRGESMIEAGIFDGDYVIARKEETARDGDMVVALVDG
                     EEATVKYFFREPDRYRLEPANQAMAPIYSHDVRILGKVIWVVRRVG"
     gene            5035..6702
                     /locus_tag="PLY56_00520"
     CDS             5035..6702
                     /locus_tag="PLY56_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ19994.1"
                     /translation="MTGLLRIFALAAALTGAASTLHAAPARPAQAPTGPRVVRTVPAN
                     GEKAADPGITTLEITFSEPMQANKYYLGPITGRELPWEGMPPVVLSPDGRTFSVPISL
                     KPNRKYGVAINAPGHMEFRSRAGKIAQPMKLFFNTAEKASGQGAPRAAAAPAPAVPER
                     FHISTPADGSEVRDRVVVTVPASSVPEGAFVSFFVDNVFVVGLAPVKGSDAVRFEWNT
                     KEPLPGRGTVESRIPKDGPHTIRAELHIGQQKQTSQVRVTLRNTIHPAEAAEGFLLRY
                     QFTPGQELEYYHQFQSQVKAPGSQQPQRHRGEIFSRILVDEKLDNGQFVLLGRFRKAL
                     VDGTEYTGSQLADKWNMSRSVRLELDPHGMMQVVTKKDHPLVEAVSQLIPLPANRVKL
                     NEPWTSSLVLPEVLKGKTAVAPAKHVAERVVWEGGQETVVIRSDYQIAGIKNPMASKH
                     LILATGTTSLPSGGELTVRGRRYTFFAYKLGRVMRVTDQKTIRLVLTQAPNTGMGMPG
                     EGMPGMEPMGMPGMPGMPGMPGRPQRQIYNIEAEEVLEFTPPTETEE"
     gene            6718..7527
                     /gene="tatC"
                     /locus_tag="PLY56_00525"
     CDS             6718..7527
                     /gene="tatC"
                     /locus_tag="PLY56_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOJ19995.1"
                     /translation="MITGLPSLWRPKQQRPEPESFDREMELTEHLAELRTRIIRCIIY
                     GTIGMAVAWFYYPSLYALLIRPIETIRSQAEWSFIFTSFLEPFFVRLQVTAVAGIILA
                     FPLISLEAWGFIAPALTRTERRAVFFVAPLCVFLFVAGVGSTLFVLPAAVRWFLGFLP
                     DETRLMQKVNDYVLFLAKSCLGFGVVFQLPVVLLFLGKIGIVNSSFLIRYWRQAIVLA
                     AGASAMITPSADAFTMMAMTAPLLVLYALSIVLVRFVERAEAKAAALMDEE"
     gene            7531..9807
                     /locus_tag="PLY56_00530"
     CDS             7531..9807
                     /locus_tag="PLY56_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ19996.1"
                     /translation="MSIRRFLAGLRASPEARGRLVCHRRIDARPARYQELAQPLPPRL
                     QEYLARQGIAQLYTHQAQAITAARAGNNVLSITATASGKTLTYNLPVIETLLSDPRSR
                     ALYLFPTKALAQDQLGKLRDLHLYPLLKPGTYDGDTPAHLRARYKRELNVVLTNPDML
                     HVGILPYHGTWATFFRNLKYIVIDEIHAYRGVFGSHVANVLRRLRRICRLYGSDPQFL
                     CCSATVANPMELMQRLLGDGREVAIIDDDGSPRGARHFLFWNPPPLGDGGRVSTNLEA
                     TQLLASLVRDGVRTILFTRARKTAELILRYARFALQDTAPELAARITSYRAGYSPEQR
                     REIERALFDGELLGVTSTNALELGIDIGNLHACIIAGYPGSIASTWQQAGRCGRGHDE
                     SLVILVALDNPLDQYLMRHPEYFFGSPPEHAIVDPGNPHILREHLLCAAYEHPLQPDP
                     DERIFGVSFRALAGELARDGKVRRAGRALRGAGSQHPARQVNIRSVFSDPYRVVLEET
                     GEILGTVDAEHALETLHPGAVYLHLGESYRVSTLDVASRKALVSPASGDHYTQPRTSV
                     DVFFERAVAQRPLGTTTLCHGPVEVVSQVVGYRRKKLFSEDVLAEVDLELPPQRFRTH
                     GVWFTLPPALTERLVAEEYDLTGSIHAVEHATIGIVPLHVLCDRMDIGGVSHAHHPDT
                     GAPTICIHDAIPGGVGIAEKAYTLLEEILRATLRAIEECPCTSGCPSCIQSPKCGNNN
                     QPLDKAGAEMLLHHLIGE"
     gene            complement(9850..11019)
                     /locus_tag="PLY56_00535"
     CDS             complement(9850..11019)
                     /locus_tag="PLY56_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ19997.1"
                     /translation="MRILEVEYLGPPAMGGVELLVEALHRKFTGAGHEVEIWCSDLAG
                     FDGSRLAPGHAEVNGMRVRRFPTRRVRPWIFDPHHLVWCGLRQALIEAAGQGAVLHVH
                     SFPSDQVLKSLSAMAAGGRVVITPHHDTESLRRYLRLWRAQVICRRLVRMARRHPRVS
                     LTVHTPAARDLWVNEIGWPEAQVRVIPNGVDLEEFDRVTPAEIDAATRYWPGCDLRVL
                     FVGRLARSKGVDTLLRALARVPGVGLLVIGPDAGAREELDRLRHELNLEERAVFATMP
                     RRELCAAFRACDLFVLPSRFGENFGIAAVEAMAARKPILVSDRGGLPSLAESGRNGLV
                     FTAESVESLADALARLAASEELRRQLGEAGRAAVEARYTWDHVARAYLDLFAAVK"
     gene            complement(11260..11622)
                     /locus_tag="PLY56_00540"
     CDS             complement(11260..11622)
                     /locus_tag="PLY56_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19998.1"
                     /translation="MSKQYIEGADFSLERLTDSVPQDGRYYLLKDSQIIAVFDSPEEA
                     QAYYKRLCLSYWTRMLGSDDLTLRLQAARGLLRRDRTHRPALETLAAYGDSKERSYAA
                     ESLRRLERQQATATPAEA"
     gene            complement(12025..12300)
                     /locus_tag="PLY56_00545"
     CDS             complement(12025..12300)
                     /locus_tag="PLY56_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ19999.1"
                     /translation="MSRRLPRHMRSKESRRQRSGAQAPRGEQTAPESPARRPAAPSTR
                     SGRQTRGGVATARRSRSAAGVTAILVAILLVAVALLYMWRFINLGGP"
     gene            12482..12655
                     /locus_tag="PLY56_00550"
     CDS             12482..12655
                     /locus_tag="PLY56_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20000.1"
                     /translation="MPGGAYKPQARRLPGLWPTSSRYGHVLQEMDDTAARRLDNLFNT
                     KQDETEQDEEGFY"
     gene            complement(12740..12826)
                     /locus_tag="PLY56_00555"
     tRNA            complement(12740..12826)
                     /locus_tag="PLY56_00555"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12790..12792),aa:Leu,seq:taa)
     gene            complement(13118..>13318)
                     /locus_tag="PLY56_00560"
     CDS             complement(13118..>13318)
                     /locus_tag="PLY56_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20001.1"
                     /translation="VVIADDDSNYRTLYCPQCSALSDWNKMPDDRHSGMVNCAFADGH
                     AKSMKKSVLYNNGNHSPYFNGK"
BASE COUNT         2289 a         4604 c         4204 g         2221 t
ORIGIN      
        1 cgcgcaggcg ctcgaaggca tccgagaaag cctcgacatc ggcgtaggcg gccgtcaccc
       61 gggtgatgaa gcaggccatc gggttcaagt cgacgccaat ggcccggcgg cccgtggcga
      121 gcgcctcgat cacggtggtg ccgctgccgc agaaggagtc caggacgaga tcgccctcgt
      181 tggtgttctc ctggatcagc ccgcgcacca cgtaccacgg ccggcggccc cagtatttgt
      241 gcgccagata ccgactctgg aagtaagatt gcttcgccac agccccctcc gggccaagga
      301 atcatacgcg ctaccgggcc ccacgcagcg caccacttcc ccagatagcc tccccagcgg
      361 gatggaacgc atccctctca gcctgacctg ggcgtgcagg agaggcggtc cactccacct
      421 cacgaccctt cactacagcg ccgcgggaca gcgatttcac ccgccgcgcc gaggccagct
      481 cgcgctctgc ccggcgcagc tccaccgcgt ccagccccag caggcgcgcc accaggtaat
      541 ccaacgcggc gcggatgccc tcctcctcgg gatgctgacg cagctcctcg cccagcgcct
      601 ccaactctcg ccgggccacg ggatccatcc gcgctgggtc gggcagctgg agaccggcca
      661 gctcatagcg ctgtatcttc agcagcccgc gtccctgccc gcgcccatgc gcctccagat
      721 gcagcgccgc caccaccgag ttgagcagcg cgaagagcac cgacgtctcc tctgggcggc
      781 gcgggatcac ctcgaagaag ttgtcgctcg ccagcaagcc cgccgagttg cggaagaacg
      841 ctttgcgcgc gcgcaccgcg tagccaaaga gaagcggcgc cggctccggc ggcgttgttg
      901 gcaggtaagc cgatgtcacg acgccctgct cgcgcagata ctccgccgcg cgcgatcccc
      961 cctcgtccgg cccgggagga aaaagcacga gccccggctg cgcgacgctc tccgtgtcca
     1021 gcccgatgat ttcgcgcggg gagcgcacca cccgcctcag atagcgctcc tcaattccct
     1081 ggcgcgcagc ctgagccgca tcgagaaaga agacacgatt attcccggtc gtgagccccc
     1141 gtcgtatgcg cgccgctgct tcgagcggca ccatccccgc cgaggccgac cggatcgccc
     1201 gcacggcgcg cgcggtggac ggatgaagga accagcggtt ccacccctcc tccgcgacaa
     1261 gctctgcctg gggtaccacc cggagccggt aaccgggccc ctccccatcg cactcgcgcc
     1321 cggctgcctc gctcgcagcg gccagccatt cggcgcccgc ggaaagcgag aggaaacgca
     1381 ccggcggcgg agcacccgca tccgtgcatc cggcgcgctt ctccaagatg gtaacgcacg
     1441 cctcgaccgt ggcatccggg aagacgtcgc gctcgaaccc gatcacgaag tggatgcgga
     1501 agtgcttcag cagaaacgcc cgcaactcgc gaccatactc tgccgagagc caggtggacg
     1561 gagtgattgc tcccagccat ccccccgggc gcacgaagcg cgccaggtgc atccaaaaat
     1621 gggcgtgcag ccctgcgcgc cggctgagcg cgccgcctct cgccttcgtc tcgagagcga
     1681 gcctctcctg gcgggtgtag gggggattca tcagcgccac atcacacagc gccgattctc
     1741 ccgccggccc cccgagctcc agatcgagga gacgcccctc cggcattccc agagatgggg
     1801 gtggcagatc caggaagtct ccccgggtga cgcgaatcgc ctccggctcc acgcccgccg
     1861 cctcggccac gcgccgacgg ccaaccgcca gcgcctcggg atcgacctcc agcccccaca
     1921 cctgccggtg ccactccgcg ttccccaacg ctgcgagccg ccgccccgca gcgagaagca
     1981 gggccccctc cccgatgcag gggtcgaaaa cctccgcgtc cggatcgtca atgcagagcg
     2041 aggccatcag atcggccacg ggatcgggcg tgaagacctg gcccagccgg cgtgccttcg
     2101 cctccttgcc gggccccgag gcatgggcgc tctctccgaa accagagacg gggccatctt
     2161 ggccgcctgg gctgctcatg ggctcttgac ctttgcacga cgaggctgcg tgaggatagc
     2221 gcgtgtgtcc cattatagca tgggaagcgg gcggtttgca agcacttgtt tgcccccacc
     2281 gcgggccctc gtcacgtgaa gcaggtggtt agcacgccag agtaagggga aagcagaggg
     2341 atggaggaaa cgccatggag tggctggcgc tgctgatcac cattatcttg ctggctatcg
     2401 tcgcgttcat cgtcgtgggc gctgcggtca agatcctgtg ggcactcctt atcggcgcca
     2461 tcgtgggtct gatcgccaac gggatcgtga gcctgatcca gccttcggga gggccgcgcg
     2521 gctgtctgga gaccgctctg gctggcgtcg gcggcagcct ggtcgccacg atcttctttg
     2581 ggcgcgaagg tctgatcccg gcggtgctcg gggctatcct ggtggtgatc gcgtggaagg
     2641 cggtcacctt cagccggcac atggagagca ggtaacgtca ccggcgccgg atctactctc
     2701 ggggccaggc acgccggggg ctggctcacc cggggctggt caagctgctg tgctggcgct
     2761 gtggaggact cgcccgtcgc gcatcacgag cgagacgcgc tttagggcgg taatatcggc
     2821 cgaagcatcg cctttcacgg ccacaatatc cgcgcgcttc ccaggcacca gcgtgccgat
     2881 ctcatcgccc aggccgagcg cctctgccgg cacgcgcgtg ctcgcctcaa tggccgctag
     2941 gggtgacatg cccatcgcga cgacggccac ttccaggcca tcgacgaaac ggtcgaagcg
     3001 agtcccggcc acgccagcat cgctcgaaag catcatcctg atcccaagct cgcgcatccg
     3061 cgcgaagcgc tcttgcaggg cgcgcagctg ttcgagttgc tctacctgca ccgcctcggg
     3121 tgcctcccgg tcgggcagga ggtcgcggta gaggcccacc atcgtgtgcc cgcaggtgat
     3181 gccgctccgg gcaatctcgc gcgccagttc cgggcggaag tcgaactctt ccgcctcatc
     3241 gcgcgccatc cagctacagt gctcgaaggt gtcgatgccg gcctgcaggc agggctgaat
     3301 cccctccacg ccaagcaggt gcgccgcgac ccgcttgcca agccggtgcg cctcggaggc
     3361 ggccaccttc agctcttcgg gggagaactg gcagcggcgg gggttgctcc cgggagtcat
     3421 catgcccccg gtcgcgacga tcttcacgta atcggcgccc cgctcgacga cgccccgcac
     3481 agcccggcgc acgtcatcga gggagtcggc gtgcagcccg cagtagtgca ggtggccggc
     3541 cgtcgtggtg atcggcggtc cgctacagag gaggcgcggg ccggagaaga gcccatcctc
     3601 gatggcgcgc cgcaccttga gagtgacgaa accccggtcg ccacagtcac gcgccgtggt
     3661 gataccggcg cgtagcaacg tctgggcatt atggagcgcg cgtagaagga gatcctggtc
     3721 gtcctcctcg gccaaccggc ggcgaaccgc agcgtggtcg ggtcctgcgt caaagacgag
     3781 gtggacgtgc gagtcgatca gcccgggcaa gagtgtctgg cccggcaggt caaggaagac
     3841 ggcgccatcc cgaagcgcgg gcggcagttc gccctggcag actgcctcga ctcgtccatc
     3901 cgccaccacc aatacgggat catccgtggg cgccgcgccc gtgccatcga tcaggcgcga
     3961 ggcgcgcagc accaaccgcc gcgcaacggc atcgctcatc agactgcccc caaaatcaag
     4021 agtgcggccc cgctcgggga gccaggacca tgcccaacgc ggtgattcgg cgcctatcca
     4081 ggccgctcct gcacggaccg cctaccccac gcgccgcacc acccagatca ccttgcccag
     4141 gatgcgcaca tcgtgcgagt agatcggcgc catcgcttgg ttggccggct cgaggcgata
     4201 gcggtcgggc tcgcgaaaga agtacttgac ggtggcctcc tcgccatcga ccagggccac
     4261 gaccatgtcg ccatcgcgcg ccgtttcctc cttgcgagcg atgacatagt cgccatcgaa
     4321 gatacctgcc tcaatcatgc tctcgccgcg cacgcggagc atgaacgtcg gatcctcgcc
     4381 cacccactcc ttcgggagag cataaaccgc ttctcgatgt tccgcggcca ggaggggggc
     4441 gcctgcggcg actcgcccaa cgaggggcac ggctacgctt gccggttggg ccagccacag
     4501 ctcctcggca tccaggatct caatcacgaa ggagttggga gtagaaatcc ggcggatctt
     4561 cccctcacgc tccagctcca ccagatggcg gtggaccgtc tcggaggagc gcaggcctaa
     4621 ggcacggcca atctcgcgca ctgtcggcga gcgatggtgt tgccgcgtaa agccgcagag
     4681 aaactcaaac acccgcgccc tcgtctcgcc ttgcctgctc aactcgccat cccttcctgc
     4741 tcctgccaac cggcccaaca caaacgatgt ttgacagcct ggcgggatct ccttcgccag
     4801 agagcgaggg gcaaacagac ggcgcgacga ataaagattc atggctttcg ccacgtcgcg
     4861 ggatgcgggc ggaaagtggg gtccgcgccc ggagctctgt tctctcggaa atctcacgcc
     4921 aactggggca tgcgttgact tctcagcggc gctatgttat catagtgccg ccatcatcat
     4981 gttgtacggc tgtagcaatc ttcgcccccg agatgggagg ccgaggaggt cgccatgaca
     5041 ggactccttc gcatattcgc tcttgcggcg gcactcaccg gggccgcatc tactctccat
     5101 gctgcgcctg cccgccccgc ccaggctccg actggcccgc gggttgttcg cactgtgcca
     5161 gccaatggcg agaaggccgc ggacccgggg atcacaacgc tcgagatcac cttcagcgag
     5221 ccgatgcagg cgaacaagta ctatctcggg ccgattaccg ggcgagagct cccttgggaa
     5281 gggatgccac cggtggtgct ctctccggac ggccgcacct tctcggtgcc gatctccctc
     5341 aagccgaacc gaaagtacgg ggtggcgatc aatgccccgg ggcatatgga gtttcggagc
     5401 cgcgccggga agatcgcgca gcccatgaag ctgttcttca acaccgcgga gaaggcttcc
     5461 gggcaggggg cgcctcgcgc cgcggcggcg ccggcacctg cggttccaga gcgcttccac
     5521 atctccaccc ctgccgacgg cagcgaggtg cgtgatcgcg tggtcgtcac ggttcccgca
     5581 tcctccgtgc ccgagggcgc gtttgtcagc ttcttcgtgg ataacgtctt tgtggtcggc
     5641 cttgcgcccg tcaaggggtc ggatgccgtc cgctttgagt ggaacacgaa ggagccgctg
     5701 ccagggcgag gcaccgtgga gtcgcgcatc cccaaagatg gcccccacac catccgcgcg
     5761 gaactacata tcggtcagca gaagcagacc tcccaggtga gggttaccct ccggaacacg
     5821 atccacccag ccgaggcagc cgaaggcttc ctgctccgct accagttcac gcccgggcag
     5881 gagctagagt actatcacca gttccagagc caggtgaagg cccccggctc gcaacagccg
     5941 cagcggcacc gcggcgagat cttctcacgc atccttgtgg atgagaagct tgataacggc
     6001 cagttcgtgt tgcttggtcg gttccgcaag gcactggtgg acggcaccga gtacaccggg
     6061 tcgcagctcg ccgacaaatg gaacatgagc cgttctgtgc gcctcgagct cgacccgcat
     6121 gggatgatgc aggtcgtcac caagaaggat cacccgctgg tggaggcggt cagccagctc
     6181 attccgctgc ccgccaatcg cgtgaagctg aacgagccct ggacgtcgtc gctcgttctc
     6241 cccgaggtgc tcaaggggaa gacggccgtt gcgcctgcca agcacgtcgc cgagcgcgtc
     6301 gtgtgggaag gcggccagga gacggtcgtc atccgatcgg attaccagat cgccggcatc
     6361 aagaacccga tggcctcgaa gcatctcatc ctggcgaccg gaacgacctc gctgccgtct
     6421 ggcggcgagc tcacggtgcg tggccggcgg tacaccttct ttgcctacaa gctcgggcgg
     6481 gtaatgcgcg tcaccgatca gaagacgatt cgcctcgtgc tcacgcaggc cccaaacaca
     6541 gggatgggga tgccgggcga aggcatgccg gggatggagc ccatgggcat gccgggaatg
     6601 cccgggatgc ccgggatgcc gggccggccc cagcgccaga tctacaacat tgaagctgaa
     6661 gaggtgctcg agttcactcc gcccactgag acggaagagt aggcttagaa gcctctgatg
     6721 atcaccggtt tgccaagtct gtggcgcccc aagcaacagc gcccggagcc cgagagcttc
     6781 gaccgcgaga tggagctgac ggagcacctc gcggagcttc ggacccggat cattcggtgc
     6841 atcatctacg gcacgattgg gatggcggtg gcgtggttct attacccgag cctctacgcg
     6901 ctgctcatcc ggccgattga gacgatccgc tcgcaagcgg agtggagctt catcttcacc
     6961 agcttcctgg agccgttctt cgtccgcctg caggtgacgg ccgtcgccgg aatcatcctc
     7021 gcattcccgc tgatctcgct ggaggcgtgg ggcttcatcg cgccggcgct cacgcgtacg
     7081 gaaaggcgcg ccgtcttctt cgtcgcgcct ctctgcgtct tcctcttcgt agcgggtgtc
     7141 ggctcgaccc tctttgtttt gcccgctgcc gtgcgctggt tcctcggctt cctgccggac
     7201 gagacgcgcc tgatgcagaa ggtgaacgac tatgtgctct ttctcgccaa gagctgcctc
     7261 ggcttcggcg tcgtcttcca gcttccggtc gttctcctct tcctgggcaa gattggcatc
     7321 gtcaattcgt cttttctgat ccgctactgg cgccaggcca tcgtcctcgc ggcaggcgcc
     7381 tccgcgatga tcacgccctc cgccgatgcc ttcaccatga tggccatgac cgccccgttg
     7441 ctggtcctgt acgctctgag catcgtcctg gtgcgcttcg tggagcgagc tgaggcgaaa
     7501 gctgccgcgc tgatggatga ggagtgatgc gtgagcatcc ggcgcttcct ggccggcctg
     7561 cgcgcatccc ctgaggcccg tgggcgtctg gtctgccacc ggcgcatcga cgcgcggccc
     7621 gcgcgctacc aggagctcgc gcagccgttg ccgccgcgcc ttcaggaata cctggctcgc
     7681 cagggcattg cccagctcta cacgcatcag gcgcaggcga tcactgcggc gcgcgcaggc
     7741 aataacgtcc tctcgatcac cgcaaccgcg agcggcaaga cgctcaccta taacctcccg
     7801 gtcatcgaga cgctcctcag cgaccctcgg tcgcgcgcgc tctacctctt ccccacgaaa
     7861 gcactggcgc aagaccaatt ggggaaactg cgcgatctcc acctctaccc gctgttgaag
     7921 cctggcacct atgacggtga cacgcccgcg cacctccgtg cccggtataa gcgcgagctg
     7981 aacgtggtgc tcaccaaccc ggatatgctc cacgtgggca ttctccctta ccacggcacg
     8041 tgggctacct tcttccgcaa cttgaagtac atcgtcatcg acgagattca cgcctaccgc
     8101 ggggtgttcg gctcgcacgt ggccaatgtt ctccgccggc tgcgccgaat ctgccgcctg
     8161 tatggcagcg acccgcaatt cctctgctgc tcggccaccg tcgccaaccc gatggagctg
     8221 atgcaacgcc tgctgggcga tggccgcgag gtggcgatta tcgacgatga cggctcgcct
     8281 cggggggcca ggcacttcct tttctggaac ccgcctcccc tgggcgacgg aggccgggtg
     8341 agcaccaacc tcgaggctac gcagctcctg gcctcgctgg tgcgcgacgg cgtgcgcacc
     8401 atcctcttca cgcgtgcccg gaaaacggcg gagctgatct tgcggtatgc acgcttcgct
     8461 ctgcaagaca cggccccgga actcgcggcc cggatcacct cctaccgcgc cggctatagc
     8521 ccggagcagc gccgcgaaat cgagcgcgcg cttttcgacg gggagctcct cggggtcacc
     8581 tccaccaacg cgctggagct ggggatcgat atcggcaacc tccacgcgtg catcatcgcg
     8641 ggctatccgg ggagcatcgc cagcacgtgg caacaggcag ggcgctgcgg gcgcgggcac
     8701 gacgagtcgc tggttatcct ggtggccctc gataatcccc tcgatcaata cctgatgcgc
     8761 cacccggagt atttctttgg cagcccgccg gagcatgcca tcgtggatcc ggggaatccc
     8821 cacatcctgc gcgagcacct cctctgcgcc gcctacgagc atccgctgca acccgacccg
     8881 gacgagcgca tctttggggt gtcgttccgc gcgcttgcag gcgagttggc gcgcgatggc
     8941 aaagtgcgcc gagccgggcg tgccctccgg ggggccggat cgcaacaccc tgcccgccag
     9001 gtcaacatcc gttccgtttt cagcgatccc tatcgcgtag tccttgagga gacgggtgag
     9061 atcctcggca ccgtggatgc cgagcacgcc ctggagacgc tgcacccggg cgccgtctac
     9121 cttcacttgg gcgaatcgta tcgcgtgagc acgctcgatg tggcgagtcg caaggcgctg
     9181 gtctccccgg cgagtggaga ccactacacg caaccgcgga cgagcgtgga tgtcttcttt
     9241 gagcgcgcgg tggcacagcg tcccctgggc acgacgaccc tctgccacgg cccggtggag
     9301 gtggtctctc aagtggtggg ctaccggcgc aagaagctgt tcagcgagga cgtcctcgct
     9361 gaagtcgatc tcgagttgcc cccgcaacgt ttccgcactc atggagtctg gttcacgttg
     9421 ccgccggctc tcaccgagcg cctggttgct gaggagtatg acctgacggg aagcattcac
     9481 gcggtggaac acgccaccat cgggatcgtg cccctgcacg tcctatgcga ccggatggat
     9541 atcggcggcg tgtcgcacgc gcaccacccg gatacgggag ccccgacgat ctgcatccac
     9601 gatgccatcc cggggggcgt ggggattgct gagaaggcct acacgcttct agaggagatc
     9661 ctccgcgcca cgctacgcgc gatcgaggag tgcccttgca ccagcgggtg cccatcctgc
     9721 attcaatcgc ccaagtgcgg taacaacaac caacccctcg ataaggccgg ggccgagatg
     9781 ctccttcacc acctgattgg agagtgatcc ccagcctgtc ggggtctcct tacgagaatc
     9841 gggcgagcgt cactttaccg cggcgaagag gtcaagatac gcccgcgcga cgtgatccca
     9901 ggtgtagcgt gcctctacgg ctgccctgcc ggcctctccg agctggcgcc gcaactcctc
     9961 ggacgcggcc agacgcgcga gggcatcggc cagggattcc accgactcgg ccgtgaaaac
    10021 cagcccgttg cgccccgatt ccgcgagcga gggcaacccg ccgcggtcgc tcaccaggat
    10081 cggcttgcgg gcggccatcg cctcgacggc cgcgatcccg aagttctctc caaaccgact
    10141 gggcaagacg aagaggtcgc acgcgcggaa cgcggcgcag agctcccggc gcggcatcgt
    10201 ggcgaacacg gcgcgctcct ccaggttcag ctcgtgtcgc aatcgatcca actcttcacg
    10261 cgcgcccgcg tctgggccga tcaccagtaa cccaacgccc ggcacccgcg cgagggcgcg
    10321 caggagggtg tctacccctt tggaacgcgc caggcgcccg acaaagagca cgcggaggtc
    10381 gcacccgggc cagtagcgag tggcagcatc gatctcggcc ggcgtcaccc ggtcgaactc
    10441 ctccaggtct acgccgttag ggatgaccct tacctgggcc tccggccagc cgatctcgtt
    10501 cacccatagg tcacgcgcgg caggagtatg cacggtgagg ctcacccggg gatggcgacg
    10561 ggccattcgg acgaggcggc ggcagatcac ctgcgctcgc cagagccgca ggtagcggcg
    10621 cagcgactcc gtgtcatgat gtggcgtgat taccacccgc ccgcccgcgg ccatcgcgct
    10681 caggctcttc aggacctgat cggagggaaa ggagtggaca tggagcaccg cgccctgccc
    10741 ggcggcttca atcagggcct ggcgaaggcc acaccagacc aggtggtggg ggtcgaagat
    10801 ccacgggcgc acccggcgcg tggggaagcg acgaacgcgc atcccgttca cctcggcgtg
    10861 gcctggcgct aggcgcgagc cgtcgaagcc agccaggtca ctgcaccaga tctcgacctc
    10921 gtggccggcg cccgtgaact ttcggtggag cgcctccacc aggagctcga ccccgcccat
    10981 cgccggcggg cccaggtact ccacctccaa gattctcact cgctgcagcc ctttcagagg
    11041 cgcggagaca accggcaacg atggtatcgc aatcgagcga ggccggcccc gttcgacgca
    11101 cacgggcgag ccatcgagcg cggcctggcg gaccccagag gaaacgcgcg ctgcttctgc
    11161 atcgccactc tcctgccgat gcagaagcag cgcttcaggc gcctcgccat ttcaggaatc
    11221 gcccgcctca gaggagtgga accgcatcct ccgggggaat caggcctccg ccggggtggc
    11281 ggttgcctgc tggcgctcca aacgtctcaa gctttcggcc gcgtagctcc tctccttcga
    11341 atcgccatag gcggccagtg tctccagcgc gggacgatgg gtacggtcgc ggcggagaag
    11401 cccacgagct gcctgcaagc gcagggtcag gtcatcactt cccagcatgc gggtccagta
    11461 ggagaggcac aatcgcttat agtaggcctg cgcctcttcc ggcgaatcga acaccgcgat
    11521 gatctgggag tctttcagga ggtagtagcg gccatcttgt ggcacgctat cggtgagccg
    11581 ctccaaggag aagtcggctc cctcgatgta ctgtttgctc actgctgcgc tcctttcaca
    11641 ggctcgcaag aaggagagct taccacacgt aggctcgcgg cgaggcaacc ggtttagtca
    11701 tggggcccga ttatagccgt cacgggaaga gaagggatag actgcctggc gtacagcatt
    11761 gcgtttcgca tgtgcgtcgc tccgcattcc agggcggcta tacagaaact gacagaaggg
    11821 atacggtcgc ccgctaccat tataccagag cccgcccagt ttgtaaagcc cgatgacgag
    11881 cgcgtgagca gcagcaccct gggtacgaag cagccggcaa gaggtgggta gaacgagcgt
    11941 gaggccgcgc tctactcttc aatcccgttt gtttcgccat cgagcccgtt tcgggaagac
    12001 ggcgcgcggc ttggaagggc gccgtcaggg gccgcccagg ttgatgaaac gccacatgta
    12061 aagtagggcc acggccacca ggagtatcgc taccaggatc gcggtgacgc cggccgccga
    12121 gcgtgaccgc cgggccgtcg ccaccccacc ccgggtctgc cggcccgacc gggtcgaagg
    12181 cgccgccggc cgcctcgccg gcgattcggg ggccgtctgc tcgccacgag gtgcctgcgc
    12241 accgctgcgc tggcgccggc tctccttgct ccgcatgtga cggggcaggc gtctagacat
    12301 cggctctcac ccttcctatc tgccgcatcc gcgtcgttcc ttcgggcgcc gccgctcgcc
    12361 cacgtcccca gggcaccttc cgatgggatg ccgatccttg aggcgatcct tcccttccct
    12421 actgcacttc aatcctagtt cttgctgcgc agcacaccct ggcccccaag cgcgggatgt
    12481 cttgcctgga ggggcgtaca agccacaggc ccggcgattg ccgggcctgt ggccaacctc
    12541 gagccgctac gggcacgtgc tacaggagat ggacgacacc gctgctcggc gcctcgacaa
    12601 cctcttcaac acaaagcaag atgagacaga gcaagatgag gagggcttct attagatggt
    12661 tattagatcg cggcatgaaa cggacaatcc gccggaatcg tggagatgcc ggaaacccgc
    12721 atcattgcta ggtttggcct ggtggggaga gcggggctcg aacccgcatg gcctctcggc
    12781 cgggagattt taagtctcct gcgtctaccg gttccgccat ccccccgcca accggattat
    12841 agcatcgtca cgactgacgc gtcaatggcg agagagagac gcgccagctg gctcaaggct
    12901 tatggtgatg cctcccgtcg gctgcgagga gccctcctgc cgtgccgcgt ggctctctcc
    12961 agatggcgga ttcgcacgca tggggtgccg ccggtcgcca cggcgagggc agcaccccag
    13021 atcccagagc gtggtcccac tcgcacgcca tccggtgctc cctacggcgc ccttctggct
    13081 cgatcgccgg gagcgggagg ctcactcgct aggtgggcta cttcccgttg aagtacgggc
    13141 tgtggttgcc gttgttgtag agcaccgact tcttcatgct cttcgcgtgc ccatccgcga
    13201 aggcacagtt gaccatcccg ctgtgccggt cgtccggcat cttgttccag tctgacaagg
    13261 cgctgcactg cgggcagtag agcgtgcggt agttgctgtc gtcatcggcg atgacgac
//
LOCUS       DAOSCV010000010        13246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3171_length_13246_cov_3.732772, whole genome shotgun sequence.
ACCESSION   DAOSCV010000010 DAOSCV010000000
VERSION     DAOSCV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 13246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13246
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3171_length_13246_cov_3.732772"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..367)
                     /locus_tag="PLY56_00565"
     CDS             complement(107..367)
                     /locus_tag="PLY56_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ20002.1"
                     /translation="MSRSVFTEAYIRFRRLLIEARRSARLTQAQLAERLGRPQSFVSK
                     YETGERRLDVVEFLELAQVLGIDPVELLREVQKAIPPSDRPT"
     gene            835..1374
                     /locus_tag="PLY56_00570"
     CDS             835..1374
                     /locus_tag="PLY56_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20003.1"
                     /translation="MGLWRYMPLIPLAFLALVPTARWLSAKLWKAISVPAVNVTLTGL
                     RWSAGQGYLQLDVHLSSDEAMRPEWRDYVAIIFAAHSVPSRPLDSYVSALAGGGTLEV
                     KAFFACRGRAPDGLSALIRLPDGRSATLPVANLQYVVGKCDACKGSGGTCKFCGGSGR
                     VDVAVMQEVGSPTDRYQLG"
     gene            1687..2325
                     /locus_tag="PLY56_00575"
     CDS             1687..2325
                     /locus_tag="PLY56_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20004.1"
                     /translation="MPVLIVVGFFIATKWRDGRASWTGQAQQPSEMPAASVEMRVHIV
                     WTGQGQGWAEGTMTNESAVALHNLGMEVARPEVVERNGFVVGVKDNWKSGTVCEIVPR
                     SIPPGGTARVAVHGSSMAEVSNLRVFYVEPKVYRYSDQQSIPYEEKHEVECTQEIRPG
                     EGVRETNAATPGGTPGNGSPSLQNPDGTFRNVPLHGYGIQKDPQHNPGGFGR"
     gene            2928..3905
                     /locus_tag="PLY56_00580"
     CDS             2928..3905
                     /locus_tag="PLY56_00580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ20005.1"
                     /translation="MKKAHGLVEMTIRSLVAEGALDAASALALRAQSARGLWGLPPAY
                     VPSPSRRSAPNRPPTREEFDITAQVVSSFLPVPATHPGLTAMVGELASRASIPTPRLW
                     ILRDEVPNACAVGFVPEEAHLMVTAGLLNSGIPERQLFAVLSHEVAHITLGHTAENTA
                     LHAKALDIQMSAALTGGAVAGAGGGLGATVASVSALSDDDCVGLLGLVVGLGIAAVGG
                     AVGAGISAQGAAAAKATLTRGLHHMEFAADARGAELSGDPQALADALAYLDRSQDAGR
                     WSGRAVGHAAHAFIVAPGVAWSLADASTHPPCSRRIAALALLRMAREAR"
     gene            4184..4486
                     /locus_tag="PLY56_00585"
     CDS             4184..4486
                     /locus_tag="PLY56_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOJ20006.1"
                     /translation="MPHKAGKDWLTTLLLCLVTGFLGGHRFYTGHITTGIIQALTMGA
                     CGLWWLADLATILAGRFRDSTGNRLVGSHYPAVTRWVIGTGVVCMILVILTMASID"
     gene            complement(4884..5567)
                     /locus_tag="PLY56_00590"
     CDS             complement(4884..5567)
                     /locus_tag="PLY56_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20007.1"
                     /translation="MGPYCQFCHTRCFVQLPEDTPEGIREAYGPATLIATCHPGQFHE
                     RERVGYCYTDILRRTGAAPKTCPVCERAMQAQGNLCLDCSGDDEGDLPEWPLWADAVV
                     EAMNSGLMECWPGGLLFGYVEAEHQVVVRPTPFHCDGDQVAYPSFFLSIEHLVALFDA
                     PPAITMCTREDDVSVEGEIGGEHVWVQIRKEPLDSDEPVGRLHPDGGFEEYGDGDDED
                     PPSPGDDRA"
     gene            complement(5608..6579)
                     /locus_tag="PLY56_00595"
     CDS             complement(5608..6579)
                     /locus_tag="PLY56_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ20008.1"
                     /translation="MRKAQLGSAKTQRGTGPAKSKTLSVSDLEREAQAWFLDGEIRQL
                     SRNTLALRRVLLDRFLRFLRENGHTHVDTTAVRAFLVSITKQGLRPKSVKNYYVDLSC
                     LLRFLQTEGVVTTNPFDAISRPRVPVDQIQPFTVDQVRALLTAAKRSRNPRRDEALLL
                     LMLDTAARASEVCGLRMSDLDLANRRCQVVGKGNKRRTLPFSAQTARALLRYLSQQER
                     DPEDAVFLCERGERTGCALSRYGLLDMVARLSRSAGLQGVRPSPHTLRHTAAIFYLRG
                     GGNVFALKELLGHTELRMVNRYLSLSQADVEAQHRQFSPVEQLRKSR"
     gene            complement(6635..6721)
                     /locus_tag="PLY56_00600"
     tRNA            complement(6635..6721)
                     /locus_tag="PLY56_00600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6686..6688),aa:Leu,seq:gag)
     gene            complement(6761..7597)
                     /locus_tag="PLY56_00605"
     CDS             complement(6761..7597)
                     /locus_tag="PLY56_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ20009.1"
                     /translation="MMGATVMRLGLSRSVEELAGDPGAPLVQWREWGISVIELRRVDE
                     ETPPEEVTRAARRIWSAGLALAIHARLPRMATGATLGKLFPGLSGVLPELRERGEEVA
                     VPIHAYAPRAESESRLAAQTREAVRRAVDAAEREEVPLRFAVELNRAKGPADPSVTYE
                     GVLALVEGLPAGICWDFGHGCSNARRGLLAATPPAAFVAAVTHTHIHDIGPSGETHWP
                     LTQGVVPVDAYVAMLASAGYGGILNLELSEERYAGQVDPAEGLRASIERLRECVKRAN
                     RC"
     gene            complement(7594..8529)
                     /locus_tag="PLY56_00610"
     CDS             complement(7594..8529)
                     /locus_tag="PLY56_00610"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015184525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ20010.1"
                     /translation="MNLRERYRGALLGLAAGDALGTTVEFRAPGSFPPVAEITGGGPF
                     RLKPGQWTDDTSMALCLAESLVERSGHDPIDQLERYVRWWRQGHLSSTGRCFNIGRGT
                     KGALRRFEQMRVAYCGSTDSRASGNGSIMRLAPVPLAYARDPRQAVILSGDSSRTTHA
                     SPECVSAARYLGGLIVGALLGATKEELLSPYYAPVPGLWDEFPLAPEVAEVASGSFHR
                     REPPEIRGSGYVVATLEAALWAFARTDNFRDGALKVVNLGEDADTTGAVYGQLAGIFY
                     GESGIPAEWRSCLSMRETIESLADRLYALAGWQEK"
     gene            complement(8587..10422)
                     /gene="typA"
                     /locus_tag="PLY56_00615"
     CDS             complement(8587..10422)
                     /gene="typA"
                     /locus_tag="PLY56_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ20011.1"
                     /translation="MTFTSAPLRDDIRNLAIVAHVDHGKTTLVDAMLRQSGLFRDNEQ
                     IPERVMDNIDLERERGITILAKNTAITYRGYKINIVDTPGHADFGGEVERALSLVDGI
                     LLLVDAAEGPLPQTRYVLSKALEAGLTPMVVINKIDRQDARPREVLDEVYELFLDLDA
                     TDEQIEFPVLYTVGRDGTAGESPEEMTGDLRPLFETLVRTIPPPRADPAAPLQVLVST
                     LDYNDYVGRLAIGRVVSGTIRVGDPVAVCKEDGSIDRTKITQLYSFEGLKRLAVDEVA
                     AGDIIALAGIEGIAIGDTVADPDEPTPLPRIRVDEPTISMLFGVNTSPFAGREGKYVT
                     SRTLRERLMKEALWNVSIRVEDTESPDSFKVSGRGELQLAILIEMMRREGYELQVSQP
                     EVITREVDGKTHEPFEHVFIDVPDAYVGIVTESLGRRKGQMTKMVNHGHGRVRMEFLV
                     PSRGLIGFRSQFLTDTRGTGLLNTLFSHYGPWQGPLPRRTNGVLVADRQGVTAAYALY
                     NLQERGQLFWGPGVDVYEGMVVGENSRPGDMNVNPCKERKASNIRSSTDQEAIRLVPP
                     RAVGLDQALEFINADELVEVTPKSVRIRKRVLSAHTRHRLEGKSE"
     gene            11330..12518
                     /locus_tag="PLY56_00620"
                     /pseudo
     CDS             11330..12518
                     /locus_tag="PLY56_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
     gene            complement(12777..>13246)
                     /locus_tag="PLY56_00625"
     CDS             complement(12777..>13246)
                     /locus_tag="PLY56_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20012.1"
                     /translation="TNIKELMEFLLRRPRPVQAAIRERLPELDRLVQDQYERDTYGRV
                     VTGPNAQPLLSAREPEPEPAPEGATAAPPAPVLLGYESPEEAYADQQRRAYLSEMRRV
                     VDQSPDDVAARLAYAAALEEAGHPDRAREEYRRVLSLSPDHAEARAALNRLGG"
BASE COUNT         2290 a         4315 c         4318 g         2323 t
ORIGIN      
        1 cagttcagca gtgtcaggta gtgtccggcc cttgggtcgt ggcgtcgtat cctatattgg
       61 gatattccga agcacgaccg aggtaatagg cggatggcgg aaatggctac gttgggcggt
      121 cggaaggtgg aatggccttc tgcacctcac gaagcagttc tacagggtcg atgccgagaa
      181 cctgggccaa ctccaggaac tccaccacgt ccagacgccg ttcgcccgtt tcgtatttgc
      241 tgacgaagga ctgtgggcga cccagacgct cggcaagctg tgcctgcgtc aatcgggcgc
      301 ttcttcgagc ctcgatcagc aggcgtcgaa accggatgta tgcctcggtg aatacagagc
      361 ggctcatggt actccaggat ggacaccaac gagcgaaatc ccggaagggg ataaaaccca
      421 aaaggggatt gccggggctt gacgaggcgg ctcccaagta gtgccgggga tcgcggggta
      481 gcggctgttg cccggactgc aggtacacga acatcgagca aggaggcacg aacagatatg
      541 tcgtcgtcgc acgagtgtag cgcgtgcggg gcagggaatt gcgcgcgcag tgcgtcctgc
      601 tcccactgcg gcaacccgtt gaatgcatcg ggaggccgga tcctgctcaa ggacaccggc
      661 aacagaaaca gcgagcctgc gccaccgcca ctggcgacgg cagtgatctc gggtggggag
      721 cgcggggccg cgtggttggt ttgggtccac tcccttctgg cttccctggc agaactggat
      781 ttcgaggagt gctgccgcat tttcgctgtc ctcctgattg tagggttttg cggcatgggc
      841 ctttggcgtt acatgcctct cattccgctc gccttcctag cgcttgtgcc tactgcacga
      901 tggctatcgg cgaagctgtg gaaagccatc agtgtaccgg cagtgaacgt gacgctcacc
      961 ggacttcggt ggtcggcagg acagggctac ctgcagctcg atgttcacct ctcctcggat
     1021 gaggcgatga ggccggagtg gcgcgactac gtggcgatca tcttcgctgc ccacagcgtg
     1081 ccatcgcgcc ccctcgactc atacgtgagt gcgctggccg gtggcggtac actggaggtc
     1141 aaagccttct ttgcctgccg agggagagcg ccggatggtc tctcggcgct catccgcctc
     1201 cccgatgggc ggagcgcgac actccctgtt gccaacctgc agtacgtagt gggcaagtgc
     1261 gacgcctgca aagggagtgg cgggacgtgc aagttctgcg gcggctccgg tcgggttgat
     1321 gtcgctgtga tgcaggaggt gggtagtccg acggaccgct accaactcgg atgaggcggt
     1381 gtcccaggcc gcagcacggt gcggcccgtg cgtctcgcaa ccgttcggtg tgactctgat
     1441 gcctgtcgcg gaggcatctg gatgaaggag aagaacgtgt ctcggatggt caagtgcgaa
     1501 aacggggaat gcggtgctcc caatcctgca ggccggacag cttgcttccg ctgtggtcac
     1561 ccgttaccgg ctgcaccggg ggagcctgaa ctggcaggtc agttcctacc cggcagggct
     1621 ttggtcacag gcactctccc cccgcaaggc cgctggcacc gtgctcgcct cactctcgtc
     1681 tgcgcgatgc ctgtgctcat tgtcgtcggc ttcttcatcg ccaccaagtg gcgtgacggg
     1741 cgcgcgtcct ggacgggaca ggcacagcag ccgagtgaga tgccagcggc ttcagtggaa
     1801 atgcgtgtcc atatcgtctg gaccggccaa ggccagggct gggcagaggg caccatgacc
     1861 aatgagtctg ccgtggccct acacaacctg ggaatggaag tggcaagacc ggaggtcgtc
     1921 gagaggaacg gcttcgttgt cggtgtcaag gataactgga agtcagggac cgtctgcgag
     1981 atagtcccga gatcgatccc gccgggggga acagcgcggg ttgcagttca cggctcttcc
     2041 atggcggagg tcagcaacct tcgcgtgttc tacgtggagc cgaaggtcta tcgctacagc
     2101 gatcagcaga gcatcccgta tgaggagaag cacgaagtcg aatgcacgca ggagatcagg
     2161 ccgggagagg gtgtgcgtga gaccaacgcg gccacaccag ggggcacccc cggcaacggc
     2221 tcgccgtcct tgcagaaccc agatggcaca ttcagaaacg tgcccctgca cggctatggc
     2281 atacaaaagg acccgcagca caaccccggc gggttcggta gatgaagtca ccactaattg
     2341 aggggatctg gtgcttaagc ccacgttcgc tgagaggacg gtcgcatatg tcggcttccg
     2401 gggtctgacg ccagagcgct ctgcgtgccc tgttcgcgtc atcctgtccc cgacgcgatt
     2461 cgagagagga gggaatcgtg cccattcggt ttgactgccc cgcctgtggc aagatgatca
     2521 tcgcacccga ctcagcatcg ggccttacgg ggccgtgccc gttctgcaga gcgacgatca
     2581 aagcaccgtc gtgtgcacgc ctgccgctgc cagaaggtca agcgccagga agagcaacat
     2641 cggctccgca ttgcactgag tccgtaggcc gcgccgttca ggctccgaca cgcacgggtc
     2701 ccgtttgcca tatctgcggt gggggcacga ttgccggggt gtgctcgtgc tgctctcgca
     2761 tcgtttgccg cgagtgtgct acacgcgagc ggaaggatct cgtttgcagc ccctgccgca
     2821 cgttgcggga tggtacaacc ggcagcaacc ccgttgcggc actccgtgcc atcgaggcgt
     2881 tgggaccaga cgtagcgcgg caccgaccgc aacgtgcgac gcgggaagtg aagaaggccc
     2941 atggcctggt ggagatgacc atccgctccc tcgttgccga aggcgcactg gatgccgcgt
     3001 ctgcgcttgc gctacgtgcc cagagtgctc gcggtctgtg gggtctgccg cctgcctatg
     3061 tgccatcgcc tagccgccgg tcagcaccga accgcccgcc tacccgcgag gagttcgaca
     3121 tcacagcaca agtcgtctct tcgttcctgc cggtgccagc cactcatccc ggactcacgg
     3181 cgatggttgg cgaactggca agccgagcga gcattccgac acctcggcta tggatcctga
     3241 gggacgaggt gccgaacgcc tgcgccgtcg ggttcgtacc ggaagaggcg cacctgatgg
     3301 tcacggcggg tcttctcaac tccgggatcc cggagcgtca gttgttcgcc gtcctctccc
     3361 atgaagttgc ccacatcacg ttgggccaca ccgctgagaa caccgccctg cacgcgaagg
     3421 cgctggacat ccagatgagt gccgcgctta ccgggggtgc tgtagcgggt gcgggcgggg
     3481 gcctcggtgc aacggtggcg tctgtaagcg ccctcagcga cgacgattgc gtgggcttgc
     3541 tcgggttggt ggtcggactg ggcatcgctg cggtcggtgg cgcggtaggg gctggcatca
     3601 gcgcccaagg cgcagcggcg gcgaaagcta cgctgaccag ggggctgcac catatggagt
     3661 tcgccgcaga cgcgagaggg gcagagctat ccggtgatcc gcaagccctc gctgacgccc
     3721 tggcctacct cgaccgttcg caggacgcgg gtcgttggtc gggccgcgcg gtagggcacg
     3781 ccgcccacgc cttcattgtc gcgccggggg ttgcgtggtc gctggcggat gccagcaccc
     3841 atccgccgtg ttcgcggcgg attgcggcac tggcgcttct gcggatggca agagaggccc
     3901 ggtgagggga aggacacagg cgatgccgat caagttcgac tgcccctggt gcagccatac
     3961 tctcacagcg cccgactcgg cggcgggggt taccgccccc tgccccaagt gcggcgtgca
     4021 gattaccgca ccggcagatg gcgaacagcc agcgggcgcg ggcaaggcca gcgctgttgg
     4081 ccccgctctc ggatcgccga cggcgggaag gacccaatcc gctcccctga ccagtccccc
     4141 gctaccatcg tcggcaccgg cccaggtatc tccctatgac cccatgccgc acaaggcagg
     4201 caaggattgg ctgaccacac tgctcctctg cctcgtcacc gggttcctgg gaggccaccg
     4261 gttctacacc ggccacatca caacggggat catccaggcc ctcacgatgg gtgcgtgcgg
     4321 cttgtggtgg ctggcggacc tcgcaacaat actcgctggc cgcttccgcg attccaccgg
     4381 caatcggctc gtcgggtcgc actaccccgc ggtgacacgg tgggtcatcg gtaccggggt
     4441 cgtctgcatg attctggtaa tcctaaccat ggcctccatc gactgaaggt aatcatctgc
     4501 tgccaagatg agagcgggga ccgacgaacg tcctactcgc cggtccccgc atcccaagag
     4561 agcgaagtcc gtaccaggcc acgagtgctg tggcccgctc gacaagggca gcggcgtccc
     4621 ccagggcgac gctcatccgc gtacctccca ccaagtccgc ctccgcgcct tcggaggtgc
     4681 gacgcagaat ggcggggcct acggtcgcca gattcctgat ggctgaccgt gtaacgctgc
     4741 tccaatgcgt ggtgttgctg cgcccgtggc ggctcctcgg cagggggttc cgccgctgcc
     4801 tcgatggccc tactccatca ccaccgtcgg tggcgcgcct tcgcgtttgg gattgccggt
     4861 ggggcgaggt tgtcgccact aggtcaggca cggtcatccc ccggtgatgg tgggtcctca
     4921 tcatcgccat ctccgtactc ctcgaatccg ccgtcaggat gcagccgccc gaccggctcg
     4981 tcagaatcga gcggctcctt gcggatctgg acccagacgt gctctccccc gatctcacct
     5041 tcgacgctca cgtcgtcctc ccgcgtgcac atcgtgatgg cgggaggcgc gtcgaagagg
     5101 gccaccaggt gctcgatgga gaggaagaac gacgggtatg ccacctggtc accgtcacag
     5161 tggaagggcg tgggccggac cactacctga tgctcggcct caacgtaacc gaacagcagg
     5221 ccacccggcc aacactccat cagcccgctg ttcatcgcct cgacgaccgc atcggcccac
     5281 aggggccact cgggcagatc gccttcgtca tccccggagc agtcgaggca caggttcccc
     5341 tgcgcctgca tcgcccgctc gcagacaggg caggtcttgg gtgcagcccc ggtcctgcgc
     5401 aggatgtccg tgtagcagta cccgactcgc tctcgctcgt ggaactgccc aggatggcag
     5461 gttgcgatca gcgtggcagg accgtaggct tcccgaatcc cctcgggggt gtcctcgggt
     5521 aactgaacga agcagcgcgt atggcagaac tgacagtacg gacccatctc tctcctctcg
     5581 ttggtaactc ggaagcggtt cccgccgtca tcggctcttg cgcaactgct caacgggcga
     5641 gaactggcgg tgttgggcct ccacatcggc ctgcgacagc gacaggtagc ggttgaccat
     5701 gcgcagttcc gtatggccga gtagctcctt gagcgcgaaa acgttgccac caccccgcag
     5761 gtagaagatc gcggctgtgt gtctgagcgt atgcggggat ggtctcacgc cctgtagtcc
     5821 tgcggatcgg ctcaacctag cgaccatatc gagcaggccg tagcggctca atgcacagcc
     5881 cgtccgctcc cctcgctcac agaggaagac cgcgtcctcc gggtcccgct cctgctgtga
     5941 aaggtagcgg aggagtgccc gtgccgtctg cgccgagaag ggaagggtgc ggcgcttgtt
     6001 ccccttgccc acgacctgac aacgccgatt cgccaggtcg aggtcggaca tgcgcagacc
     6061 acatacttca ctggcccgcg ctgcggtgtc cagcatcaga agtagaagcg cttcgtcgcg
     6121 cctggggttc cgagaacgct tggcggcagt gagtagtgcc ctgacctgat ccaccgtgaa
     6181 cggctgaatc tgatccacgg gcactcgcgg gcggctgata gcgtcgaacg ggttggtggt
     6241 aactacaccc tcggtctgga ggaaccgtaa caggcaggag aggtccacat agtagttctt
     6301 gaccgatttg ggtcgcaggc cctgcttcgt gatggagacg aggaacgccc gcacggcggt
     6361 ggtgtccacg tgggtgtggc cgttctcgcg caagaagcgc aggaagcggt cgagcagtac
     6421 gcgacgaagc gcaagcgtat tcctcgacag ttggcgaatc tcgccgtcga ggaaccaagc
     6481 ctgcgcttct ctctcaagat ccgagacact gagtgtcttc gacttcgcgg gtccggttcc
     6541 gcgctgcgtt ttggcggaac ctagctgtgc ttttcgcatt tcgccctctc ttccgagagc
     6601 ggcgagaacg ctggaacccg cgtcgttatt agtctggtgc ggaaggcggg acttgaaccc
     6661 gcatgaggtt aaccctcact aggccctcaa cctagcgcgt ctgccattcc gccacttccg
     6721 cgtggcaaca cccagattat atcactgcct ggccgctctg tcaacagcga tttgcgcgtt
     6781 tcacgcattc ccgcaggcgt tcgatgctgg cgcgcagccc ctcggcggga tccacctggc
     6841 ccgcgtagcg ctcttcgctc agctccaggt tcaggatgcc tccatatccg gccgaggcga
     6901 gcattgccac gtaggcatcc accggcacca cgccctgcgt cagcggccag tgggtctccc
     6961 cactaggacc aatatcatgg atgtgcgtat gcgttacggc ggcgacaaac gccgccggcg
     7021 gagttgcggc gagcagaccg cggcgcgcgt tgctgcagcc gtggccgaag tcccagcaga
     7081 tgccggcggg aagcccctcc accagcgcca gaacgccctc ataggtgacg ctcggatctg
     7141 ccggaccctt cgcgcggttc agttccaccg cgaagcgcag cggcacttcc tcgcgctccg
     7201 ccgcgtctac ggcccggcgc accgcctctc gcgtctgcgc tgccaggcgt gattcgctct
     7261 ccgcacgcgg cgcgtaggcg tgaatcggga cggccacctc ttccccccgc tcgcgcagct
     7321 ccgggagcac gccggacaag cccgggaaaa gcttcccgag ggtcgcaccc gtggccatgc
     7381 gcggcagccg ggcgtggatg gccagggcca gcccggcact ccagatgcgc ctggcggcac
     7441 gcgtgacctc ctccggaggc gtctcctcat ccacgcgccg cagctcgatg acggagatgc
     7501 cccactcgcg ccactgcacg agcggagccc ccggatcgcc cgccagctcc tccacggaac
     7561 gcgagagccc caggcgcatc acggtggccc ccatcatttc tcctgccatc ccgccagcgc
     7621 atagagccgg tcggccagcg actcaatcgt ctcgcgcatg gagaggcaag agcgccactc
     7681 ggcagggatg ccggactcgc cgtagaagat gcccgcgagc tggccgtaca cggcgccggt
     7741 tgtgtcggcg tcctcgccca ggttcaccac cttgagcgcg ccatcgcgga agttatccgt
     7801 ccgcgcgaag gcccacagcg ccgcttccag ggtggcgacg acgtagccgc tcccgcgaat
     7861 ctccgggggc tcacgccggt ggaaggagcc gctggccacc tcggcgacct cgggggccag
     7921 ggggaactcg tcccatagcc ccggcaccgg cgcgtagtat ggagagagga gctcctcctt
     7981 ggtcgcgccc agcaacgcgc cgacgatgag cccacccagg tagcgggcgg cgctgacgca
     8041 ctccggggat gcgtgggtcg tgcgcgagct gtcgccactc aggatgaccg cctgtcgcgg
     8101 gtcgcgcgca taagccaggg gcacgggcgc caggcgcatg atcgagccgt tcccggaagc
     8161 gcgggagtcg gtggaaccac agtaggcgac gcgcatctgc tcaaagcggc gcagcgcccc
     8221 cttcgttccg cgaccaatgt tgaagcacct gccggtgctg ctcaggtggc cttggcgcca
     8281 ccagcgcacg tagcgctcga gctggtctat cgggtcgtgc ccggaccgct cgaccaggct
     8341 ctccgccagg caaagcgcca tcgatgtgtc atccgtccac tgccccggct tcaagcggaa
     8401 cggtccgccg ccggtgatct ctgccaccgg cgggaaactg cccggcgcac ggaactccac
     8461 ggtggtgccc agcgcatcgc cggccgccag gcccagcagc gcgccccggt atcgctcgcg
     8521 caggttcatg ggtcctccca actggcgtca tggagtcttg gggggcccca tcactccatt
     8581 cgcccgctac tccgacttgc cttcgagacg atgccgcgtg tgcgcggaga gcacccgctt
     8641 gcggatgcgc acgctcttgg gcgtcacctc caccagctcg tcggcgttga tgaactccag
     8701 cgcctggtcc aggccaacgg cgcgtggcgg caccaggcga atcgcctcct ggtcggtgct
     8761 cgaccggatg ttcgacgcct tgcgctcctt gcacgggttc acgttcatgt cgcccggccg
     8821 ggagttctcg ccgacgacca tcccttcata gacatctacg cccgggcccc agaagagctg
     8881 gccacgctcc tgaaggttat agagggcata cgcagcggtc acgccctgtc ggtctgccac
     8941 gaggacaccg ttggtgcgcc gaggcagcgg tccctgccac gggccataat gggagaagag
     9001 cgtgttcagc aggcccgtgc ccctcgtatc tgtcaggaac tgcgagcgga acccgatcag
     9061 cccgcgcgag ggcaccagga actccatgcg cacgcgcccg tgtccgtgat tgaccatctt
     9121 tgtcatctgg cccttgcgcc gtcccaggga ttcggtgacg atgcccacgt acgcatccgg
     9181 gacatcgata aagacgtgct cgaacggctc gtgcgtcttg ccatccactt cgcgcgtgat
     9241 cacctcaggc tgcgagacct gcagctcgta gccctcgcgg cgcatcatct cgatcagaat
     9301 ggccagttgc agctcgccac gcccggaaac tttgaacgag tcgggcgatt cggtatcctc
     9361 cacccggatg gagacgttcc agagcgcctc cttcatcagg cgctcgcgca gggtgcgcga
     9421 agtgacgtat tttccctcgc gtccggcgaa gggggatgtg ttcaccccga agagcatcga
     9481 gatcgtcggc tcatccaccc gaatgcgcgg caagggggtg ggctcatccg gatcggccac
     9541 ggtatcgccg atggcaatcc cctcgatgcc ggcaagcgca atgatatcgc ctgcggctac
     9601 ctcgtccacg gccaggcgtt tcaggccttc aaaggagtag agttgcgtga tcttggtgcg
     9661 atcgatgctc ccatcttcct tgcataccgc cacaggatcg cccacgcgga tcgtgccgga
     9721 aacgacgcgc ccaatggcga gccggcccac atagtcgttg taatccaggg tggagaccag
     9781 cacctgcagc ggcgccgcgg ggtcggcgcg tggcggcggg atcgtccgca ccagcgtctc
     9841 gaaaagcggg cgcaggtcac cggtcatctc ctctggcgat tcgcctgccg tgccatctcg
     9901 ccccacggtg tagagcaccg ggaactcaat ctgctcgtcg gtggcgtcaa ggtccaagaa
     9961 aagctcgtac acttcatcga gcacctcccg ggggcgcgca tcttgccggt cgatcttgtt
    10021 gatcaccacc atcggggtga ggccggcctc cagcgccttg ctcagcacgt agcgcgtttg
    10081 gggcaaggga ccctcggcgg catccaccag cagcaggatg ccatccacca ggctgagcgc
    10141 gcgctccacc tcgccgccga agtcggcgtg cccgggcgta tccacaatgt tgatcttgta
    10201 gccccgatag gtgatcgcgg tgttctttgc caggatcgtg atcccgcgct cgcgctccag
    10261 gtcaatgttg tccatgacgc gctcgggaat ctgctcgttg tctcgaaaga gcccactttg
    10321 ccggagcatc gcgtcgacta gcgtcgtctt cccatggtct acatgcgcga cgatcgccag
    10381 gttgcggatg tcatcccgga gcggggccga tgtgaacgtc aagtacgcta ctcctttcac
    10441 gaagcgcatc gcatgcggag gcatgcctga agcgtcttcg cgttcccgaa tttccaacga
    10501 aaatcgtacc acaggcgggc gtgcgcgtca acaagaaaat ctcttgccgc ggctgtggag
    10561 cacgcccttg gaagagcacg cgtacccaat acccccgcgg catgggcgtt gcccgccggc
    10621 gccaagctcc gcgggcaacg cccctccacg atgcatagat gcctcgactt gcccggcatg
    10681 acaggggccg gcgcagtcat agagccgcgg acgagatgtg ctgttagcgg gcaccggtat
    10741 tacggatgtc ctctcgtgcc atttctcgcg gcgatagagc gcgggtatat gggcaaatat
    10801 tgacaaatac gggcactggg ggtataatcg tgtgtagagg aagcggtgac tacaagagga
    10861 tgctcagcgg caccgcacgc ggcgacctgg gcaaccgcgg acacaagcgg gcgtggcttg
    10921 cagccatcgc tccacgatag gggaggtatg cgccgtggat gagtattgga ccatccggca
    10981 ggcggcagaa gcgcttcagg tgcatgagcg caccgtcagg cgctggatcg ccgcgggccg
    11041 cctggccacg gttcgcgtgg cgcggacaat ccgtgtcccg cggcctgcgg tcgagcggct
    11101 ggcattagag tcgctgaggg caccgaggca ggatgccccg ggggggctgc aagcgcagga
    11161 gccctggggc gcagaacagg agcctcaatc tccagaagtg gccaccatgc cgggagagga
    11221 tctggagcac ggatctggcg ccgggccggt gcagatagat tcaactcagg ccggagagag
    11281 cggggcagga gagtgcagac cgggcgatag cgggaagagg gcaacgaaga tgtcagaaac
    11341 gaaggctgat gtagtcatac agcgagggct aaaggatgtg attgccggcg agtcatccat
    11401 ctgcttcatc gatgggcaga agggccggct gatgtattgc ggttttgacg tggcagatct
    11461 tgcgacctac tccacctttg aggaggtggc ctacttgctg tggtatgggc ctgcccggca
    11521 agatcgagtt tgaggccttc ctcgatagct tcacgggaag cgtcgagctg ccggtggaga
    11581 ccgtcatgat cttgcgcatg ttccccaggg cggcaacgcc tatggaggtg ttgcgcaccg
    11641 cggtctcctc gctggggcac tgggatcccg acagcggctg cacccggctc gacgccgttc
    11701 tgcgcaaggc gatccgcctc accgcacgca tcccgttgct cgtcaccgcg caccagcgcc
    11761 tgcgctttgg ggccgagccg atccacccgc tgccaggcaa gagcatcgcc tacaacttcc
    11821 tctatacgct ccatggcacc gagccggatc ccacctgggt tcgcgccttt gatgtggcgc
    11881 tcattctcca tgccgaccac gaatggaacg catccacctt cgccgcgcgt gtcatcgcgt
    11941 ccaccatggc ggatatgtac tccggcgtga ccggcgccat cggcgccctc aagggacctc
    12001 tccacggcgg cgcgaatgag caggtgatga agatgctcga cgaaatcggc gacccgtcca
    12061 aggcggaggc gtgggtgaag ggcgcgctcg cgaagaaggt gaagatcccc gggttcggcc
    12121 atcgcgtgta ccgcacggag gatcctcggg cgactatcct gcggcgctac gccgaggcgc
    12181 tcgccaagga agcgggcgag gaacgcctct acgagacggc tcgcgaggtc gagcgcgtcg
    12241 ttcgcgagaa caccaaggtt tatccgaatg tcgacttcta ttccggtatc tgctaccggg
    12301 tgatgaagat cgaaccggaa ctcttcacgc cgatctttgc gatcagccgt gtggttggct
    12361 ggacggcgca tatcctggag cagtggggca acaaccgcat catccgcccg cgcgcccact
    12421 acaccggcgc cgatgacctg gagtacatcc ccctcgacca gcgcggcaag acctgggccc
    12481 acgaggaaga cgatcaggat cgggagccgg atgtgtagag ccggcatccg ccatcaatcg
    12541 gatgccgtgg accggcgcgg gtcggtgctc ccgcgccggt ccctctctcc acgatgaggg
    12601 gagaggacgg aactctgaac cggcgagcgc gcgcacagtt tcggctgact ggcacaaagc
    12661 gtcggcccct gccggttccg agcttctcta ggcgcgcgcg gcggattcac cggcggctgc
    12721 cggcgcgcga ggcaccgtgc tcttccggtg ggatggcgtg tgattgcggt gttccgctac
    12781 cctcccaggc ggttcagggc ggcccgcgcc tcggcgtgat ccggcgagag cgacagcacc
    12841 cgccggtact cctctcgcgc gcgatccgga tgcccggcct cctccagggc ggccgcgtag
    12901 gcaaggcgcg cggcaacatc atccggactt tgatccacca cccggcgcat ctcgctcagg
    12961 taggcccggc gctgctggtc ggcgtacgcc tcctcgggtg actcgtagcc aagaagaacc
    13021 ggcgcgggcg gcgccgctgt cgctccctcc ggagcgggtt ccggctcggg ctcgcgtgcc
    13081 gatagaagtg gctgggcgtt cggaccggtc accacgcggc cgtaggtatc gcgctcgtac
    13141 tggtcctgga cgagtcggtc caactccggc agccgctctc gaatcgcggc ctgcacgggg
    13201 cgcgggcgcc tgaggagaaa ctccatcagc tccttgatgt tcgtcg
//
LOCUS       DAOSCV010000011        12852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3288_length_12852_cov_3.468000, whole genome shotgun sequence.
ACCESSION   DAOSCV010000011 DAOSCV010000000
VERSION     DAOSCV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 12852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12852
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_12852_cov_3.468000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLY56_00630"
     CDS             <1..275
                     /locus_tag="PLY56_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ20013.1"
                     /translation="VRIEVEATGGALLILADQFYPGWRATVDGEPAPLLAANYVLRAV
                     PVPAGRHTVTMTYRPATFLFGLYLSLAAASLLLAVATATALQTRAR"
     gene            450..1871
                     /locus_tag="PLY56_00635"
     CDS             450..1871
                     /locus_tag="PLY56_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20014.1"
                     /translation="MRYRLARNRRSKLRCAGLLLTLLLLGARAERVEAWGPHAEITKA
                     ALAVLPERARWEELLGEENLTALIAYSWLPDQQGEDLGSFYADDYLLIPAHPRYPPHG
                     MPGVRATYPAYFRRALQALRTETPVNACRQLGPLIHLVEDSGAPPHARPNTPHHSELE
                     NWIRTDAITIAGYRPRLLGRTDDEALAALQRRMDQLIAFAAERAERALPLVSAPKPNR
                     AQVEPILLEAALECARVVADLLHTLFTLGLAPQPEGASLSGTVTAAEIPLRNDHGARI
                     VLLGTEFGTLAVTAEEMPAAGWRGAYHLRNLPEGTYRVLAYRTGSRPQVSDPITLVAG
                     KTAHLDFTLPSTEPAGNLVENPDVTLSYLQEGAPDRWRRVQVGGKRVWQSAPARVKSG
                     VSYRLGAALKDPAARVHFRFLLRVMKREWNATLVSLPPGETAPAEKRFAPNILHSAVQ
                     VLVETDKPLAEAIDRVWVVPEVE"
     gene            2051..5056
                     /locus_tag="PLY56_00640"
     CDS             2051..5056
                     /locus_tag="PLY56_00640"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOJ20015.1"
                     /translation="MRPTCRSLSEAASVTRVVMLVRALMAVALGTALGLPPVNGAAKR
                     EETAPPMKAATAVRLEGSLTLDGKLDEPAWEKAPVHTGFEMPLSTANRQPIPEELQTS
                     FRVLYDDTALYFGVRCNEPRPEELVVHAARKHDAAMWSDDDIEIFLDPVGDRTEFYQF
                     AINSEGTQVDIYCQESGNIHRAEWSSLWQAAVHRGSDFWSVEVVLPFAVFHHRPAKMW
                     SDTWVFSLSRTRSVAPRYYSQFSPAQGYHDVKNFGTLEGIRVNKEAYNLYAESPQFRL
                     EPSEGGYAVLASLMVENRGDQAFEGSLGMEILAAGAQGSRVPLKLKPGQKARVEVPGG
                     RVAEQGKWPVAFRAESAGGTGLLAARFDTWLTYTPVRIKVTRPNYRNTIYATQQVDAI
                     QGYLELGIPLANVKGSIARVTLFSSLLQPRTCEVKIETARVPFELPAAGIPEGRHLLR
                     AEILRPASRDKGGEGQPEVIAAAEVPLRKLPPAPAVEARIDDQGNLLIDGSPVFIRGW
                     YGSTRYIVSAASFPQAQLPHSTNYLMGASEYEQMNMGLYTLCGITREIDEAKAKLDQP
                     IDDALKARLRPIIASVRHRRNVIGYYISDEPECRGLSPVFLKSLYEFLAEEDPYRFCK
                     IVSRAPVEYLETCDVICPHPYMNPQIAEDGGRVFGAPLRTIHNNITDACAANDGSKAV
                     WCMPQTFSYGGLRGQHPDFKESRWFALTALACGAKGLVPFIFNSYWNHRPNRIAMDYI
                     FEEVAFLAPAWLARDSATEVTADSPSVDVIAKYYKPEGAARGHFFIVAANQSLDPVQV
                     SFTVPLMGKQKSARVLVLRENRVIPVRDGKFTDELEGLGARIYTTLEVLPDFRFLDEI
                     EDEIATAIERPALAGNILAEGAVKWTIGDSEGTFSCDSDLADAVSSAAGWIPVYGDRS
                     QCVIRFEKPVVFSRVEFCSPTIASADLDIWQDNTWKTIHQWKDEYGRRLEYKGKRVRT
                     DRIRIRPTAARQGFGTSALYEITELGLYR"
     gene            5182..6921
                     /locus_tag="PLY56_00645"
     CDS             5182..6921
                     /locus_tag="PLY56_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20016.1"
                     /translation="MTTIRASARRPGLASALVLFVACALLAFLASPPALAGGLNDFFK
                     KGSKESQSSSKSSESASGKNRPESGTAREKKSPFSDSDTDIWGKPGKRETGTPGKSGG
                     LLDLFKKSGKPQPAPHGSQPDGSLPPVSYERVQPGSGGLIDLFRKPGRPQPAPSTSTS
                     PPPAVVIYHYYDPWFYPYDRFFYRYYGPYIIREREYYFGGWPYHYERPDPQEEAEESA
                     NEALRNIERGWRESRYALIARHVDDDESIAVFHDGDYSHAMTAREFRALTLQAFEDVQ
                     TVRFRFFETRMAGTTQCMADGEHVFIGPDGTERRVELQYMLRKKGRRWYIHSIDLRQE
                     RLLSSLRAPEQVLPLLSLASFKPAVDLDEETTEARAYTGLEEELLGPPAIPAPSGPRV
                     TLASITSQVATDEATPYPAAIVVASAAPVAVRRPVAESAIRITCESKPVRMAAVSGAR
                     NPVKVATVTCVVVQAAPGSTLVPGSRLTCDVNVRSTKDGLAWSVLHRGETRTLEAGTI
                     PWAQLQPARTGHLRVVVDRVATPGTAATPGEQRHTLYRKSDWDYQVALVASPKPASSQ
                     TARTELLVAPKTR"
     gene            7389..7724
                     /locus_tag="PLY56_00650"
     CDS             7389..7724
                     /locus_tag="PLY56_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOJ20017.1"
                     /translation="MIIADDVRVARSWWNRFCGLMLVPALAAGKGLLIERCRSIHTHF
                     MRFPIDALFLDRDGVVVAAVERMRPWRIGRYYPRACAVLELPAGTIASTETRPGDRVC
                     FEDGGGRVV"
     gene            7794..8150
                     /locus_tag="PLY56_00655"
     CDS             7794..8150
                     /locus_tag="PLY56_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ20018.1"
                     /translation="MSQVDLLINVTREAGGIPVIRLEGEVDIYTCPQLKETILRLIDD
                     GEYHIIIDMEKVPYIDSAGLGVLVGARRRVGEHDGSISIVNPNPSVYKVLEITRLTKI
                     FEVYQDKEGALTALGR"
     gene            8259..9365
                     /locus_tag="PLY56_00660"
     CDS             8259..9365
                     /locus_tag="PLY56_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ20019.1"
                     /translation="MRLSHLITLCNRIAPETLAEEWDHVGLQCGDPEAEVSKLLLCLD
                     LTDAVLAEARALDAECILSHHPLLFRPVFSLREDRYPARLLAGLIRSGIAAYAMHTNL
                     DSTRPGTSDALAEALGIVETEPLVPAARPAETAHYKLAVFVPAADADRVRKALGDAGA
                     GALGNYSHCSFSARGTGSFRPLEGAHPAIGSIGTQEYVPEEKVEVIVPKGILPRVLDA
                     MLAAHPYEEVAYDLLPLVPPPTGAGLGRFGRMETPCTVEELAERARACLPTSRVALAG
                     DPGRRVERVAVCGGAGGDLVDAAAAAGADLLLTGDVKHHQAHHALERGLAVIDAGHYG
                     TERPVLDLLARLLRENAGDALEVHLSRVCTDPFT"
     gene            9636..>12852
                     /gene="secA"
                     /locus_tag="PLY56_00665"
     CDS             9636..>12852
                     /gene="secA"
                     /locus_tag="PLY56_00665"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ20020.1"
                     /translation="MSILTHITKLMFDGNEREVRRLQGIVDQVNALEPELLALSDDEI
                     RARGPMFRERIQESLAKRLTPEEREQQGPEYVAALQDVLTDMLPEVFATVREASRRTL
                     GEGRPPTDPRAMRHFDVQIMGGVVLHQGRIAEMKTGEGKTLVATLPVCLNALSGDGVH
                     VATVNDYLAKRDARWNGPIYHMLGLSVAVIQSVPHESAYLYDPEYVSDDPTMHQLRPI
                     TRREAYLADITYGTHAEFGFDYLRDNLEFDEQRLRQRPLNFAIVDEVDSILVDEARTP
                     LIISGFAEESTELYTRVDRVVRSLTAERDYTVDEKQRTATLTDEGVARVERGLGIQNI
                     ADDMRVLGIVNASLKAHYVFRKDIDYVVYQHKDESGRRGEQEIVIVDENTGRLMFGRR
                     YSEGLHQAIEAKENVRVQHESQTIATITYQNYFRMYRKLAGMTGTAKTEEGEFRKIYN
                     LDVVQIPTNRPMIRIDHPDVVYKTEEAKFRGCVADILQCFCRKQPVLVGTRTIEVSER
                     LADRLSADNLQLMAMIWLLRDRVYGDKALSKEEKAEKHAFLNRKILYSADRARLTPEE
                     RRERFESVRDLWPMAKELGVSTDVLSEENVDRLAAILEVQNKRRLQMALTYGIPHSVL
                     NAKYHEKEAQIIAEAGRAGAVTIATNMAGRGVDIILGGTPDPNADGSGRLLPTDIRDM
                     TALAKKLHAARDPLTRYLQGRLSAETRQLMEIDLYEGAMAASEKLEAALVEELNRVIL
                     GPSIYDPQRFQHLMLSDEAREMVAHDGPLSPEQTAWLNRRLLESAFPREIARAYSPAE
                     AALVREVGGLHILGTERHEARRIDNQLRGRAGRQGDPGSSRFYLSLEDELWRLFGDRG
                     HALLGSWPEEEPVEAKLLTKAIARAQKKVEERNFGIREHTLKYDDVMNEQRRVIYEQR
                     RRILLGGRVWNGVHYPPVDLRANIMESAQELIVDAVNTHCPPEVAPNEWDIPGLYRSL
                     HDIFEVSRFLHESDLYGKEPNELIELLVQTAERVYAEREQVFTPEIVRELERNIFLHV
                     VNEKWVAHLDAMDYLREGIHLRAYAQVDPLVAYTKEAYEMWQ"
BASE COUNT         2251 a         4110 c         4265 g         2226 t
ORIGIN      
        1 gggtgcgcat cgaggtggag gcgaccggcg gcgcgctcct gatcctcgcc gatcagttct
       61 atccggggtg gcgggccacc gtggatggag aacccgcgcc tctcctcgcg gccaactacg
      121 tcctccgcgc tgttcccgtg cccgccggcc ggcacaccgt cacgatgacc taccggccgg
      181 ccaccttcct cttcggcctc tacctctccc tcgccgccgc cagtctcttg ctcgcggtcg
      241 cgaccgcgac cgccctccag acccgcgctc gttgagcccg cggtgacctc gggtgccggg
      301 ggaggcggca ggagtgcgcg ggctgatggg gaacaaggga ccatgccgcg gccattgccc
      361 ggatttcgcg gctggccata gtcccggcga cagaccgggt ggcgacatgt gatgatccgg
      421 tgcatgtggc aaggcaagga cacccggaga tgcgataccg tttggcccga aaccgccgca
      481 gcaagctgag gtgcgctggc ttgcttctca ccctcctcct gcttggtgct cgcgccgaaa
      541 gggtggaggc gtggggcccc catgcggaga tcaccaaggc ggcgcttgcc gtgctcccag
      601 agagggcgcg ctgggaggag ctgctcggcg aggagaacct gacggcgctc atcgcctatt
      661 cctggctgcc ggatcagcag ggcgaggacc tggggagctt ctacgctgac gactacctgc
      721 tcatccctgc ccatccacgc tatccccctc atggaatgcc cggagtgcgc gccacctacc
      781 cggcctactt ccgccgggcg ctgcaggccc tgcgcaccga gacaccggtg aatgcctgcc
      841 gccagctcgg cccgctgatt cacctggtcg aggactcggg cgcaccgccc cacgcgaggc
      901 ccaacactcc gcatcatagc gagctggaga actggatccg gaccgacgcg atcacgatcg
      961 ccggataccg cccgcgcttg ctggggcgca ccgatgacga ggcgctggcg gcgctgcagc
     1021 ggcgcatgga ccaattgatc gccttcgccg cggagagagc cgagcgggcg cttcccctcg
     1081 tatccgctcc caagccgaac cgcgctcagg tggagccgat tcttctggag gcggccctgg
     1141 aatgcgcgcg cgtggtggcc gacctgctcc acaccctctt tacgttgggc ctggcgccgc
     1201 agcccgaggg cgccagcctg tcgggcaccg tcaccgccgc cgagatccct ctgcgcaatg
     1261 accatggggc gcgcatcgtg ctcctgggca cggagttcgg cacactcgcg gtgacggcgg
     1321 aggagatgcc ggcggccgga tggcgtggag cctaccatct gcgcaacctg cctgagggca
     1381 cttaccgcgt gcttgcctac cgtacgggct cgcggcccca ggtatccgat cccatcacct
     1441 tggtcgcggg caagacggcc cacctcgatt ttacgctgcc ctctacggaa ccggcgggca
     1501 atctcgttga aaaccccgat gtgaccctct cctatctgca ggagggagcg cctgatcgct
     1561 ggcggcgtgt ccaggtgggc gggaagcgcg tctggcaaag cgcgccagcc agggtgaagt
     1621 cgggcgtctc ctatcgcctg ggggcggcgc tgaaggaccc ggcggcccgc gtccacttcc
     1681 gctttcttct cagggtgatg aaacgggagt ggaacgccac tctcgtttcc ctgcctcctg
     1741 gcgagaccgc tcccgcggag aagcgtttcg ctcccaacat ccttcactcg gcggtgcagg
     1801 tcctcgttga gaccgacaag ccgctcgccg aggcgatcga ccgcgtatgg gtcgttcctg
     1861 aagtcgagta gagtggaagg ccggaacacc ggcgcgaaac gcgcgagagc cgggcgaaac
     1921 tccatccggt agggactccc atcttcgcgg gaggacaggg gcgccgtggt gaccgggccc
     1981 tctggcaggg aacggaaagc ctggacgcga agagtacgct accgatcaca gccctcgaaa
     2041 ggagcctcca atgcgaccaa cctgtcgatc cctatccgaa gcagcctcgg tcacccgggt
     2101 ggttatgctc gtgcgcgccc tcatggcggt cgctctgggg acggcccttg gcctgccgcc
     2161 ggtgaatggc gctgccaagc gggaggagac ggcgccgccg atgaaggcgg cgacggcggt
     2221 ccgtctggag ggcagcctaa cgctagatgg gaagctggac gagcctgcct gggagaaagc
     2281 accggtgcac accggttttg agatgcctct cagcacagcc aaccgccagc cgatccccga
     2341 ggagctacaa acctccttcc gcgtgctgta tgatgacacc gcgctctact tcggggttcg
     2401 ctgcaacgag ccccggcccg aggagctggt ggtgcatgcc gcgcggaagc acgacgcggc
     2461 gatgtggtcg gatgacgata ttgagatctt cctcgatccg gtgggtgacc gcacggagtt
     2521 ctaccagttc gccatcaact ccgagggcac gcaggtcgat atctactgcc aggagtccgg
     2581 caacatccat cgcgccgagt ggtcctcgct ttggcaggcc gcggttcatc ggggcagcga
     2641 tttctggtcg gtggaggtgg tcctcccgtt cgcggtcttc catcatcgtc cggcaaagat
     2701 gtggtccgac acctgggtct tctccctctc gcggacacgc tccgtggccc ctcgctacta
     2761 ctcgcagttt agcccggcac aaggctacca cgacgtgaag aactttggca cgctcgaagg
     2821 gatccgggtg aacaaggagg cgtataacct ctacgcggag tcgccgcagt tccgtttgga
     2881 gccgtcggaa gggggctatg ccgttctcgc gtcgctgatg gtggagaacc ggggtgacca
     2941 ggcgttcgag gggtcgctgg gcatggagat cctggccgcg ggcgcgcagg gaagtcgggt
     3001 gccgctgaag ctgaagcccg gccagaaggc gcgcgtggag gtgcccgggg ggagagtggc
     3061 ggagcagggg aagtggcccg tcgcgtttcg cgccgagagc gcgggcggca ccggcctcct
     3121 cgcggcgcga ttcgatacgt ggctgaccta cacgccggtc cggatcaagg tgacgcgccc
     3181 gaactatcgc aacacgatct atgccacgca gcaggtggat gcgatccagg gctacctgga
     3241 gctggggatt ccactggcga acgtcaaggg atcgatagcc cgagttacgc tcttctccag
     3301 cctgctccag ccgcgcacgt gcgaggtgaa gattgagacg gcgcgcgtgc cgttcgagct
     3361 tccggcagcc ggcatccccg aggggcgaca tctcctgcgt gccgagatcc tccggccggc
     3421 gagccgggac aagggtggcg aggggcagcc cgaggtgatc gcagcggcgg aggtcccctt
     3481 gcgcaagctc ccacccgcgc ccgccgtcga ggcgcgcatc gatgaccagg ggaacttgct
     3541 tatcgatggc agccctgtct tcatccgggg ctggtacggc agcacgcgct acatcgtgag
     3601 cgccgcgtcc ttcccgcagg cccaactccc acactcgacc aactacctga tgggagccag
     3661 cgagtacgag cagatgaata tgggcctcta cacgctgtgc gggatcactc gggagatcga
     3721 cgaggcgaag gcaaagctgg atcagccgat tgatgacgcg ttgaaggcgc gcctgcgccc
     3781 catcatcgcc agcgtgcgcc atcgccgtaa cgtgatcggc tactacatct cggacgagcc
     3841 ggagtgcagg gggctctcgc cggtcttcct gaagagcctt tatgagttcc tcgccgagga
     3901 agacccctac cgcttctgca agattgtctc gcgtgcgccg gtggagtatc tggagacgtg
     3961 cgacgtcatc tgcccgcacc cctacatgaa cccgcagatc gcggaggatg gcgggcgcgt
     4021 tttcggtgcc ccgcttcgca ccattcacaa caacatcacg gacgcctgtg ccgccaacga
     4081 tgggagtaaa gcggtctggt gcatgcccca gaccttctcc tatggcggcc tgcgcgggca
     4141 gcaccccgat ttcaaggagt cgcgctggtt tgccctgacg gcactggcct gcggcgcgaa
     4201 gggattggtg cccttcatct tcaacagcta ctggaaccac cggccgaatc gcatcgcgat
     4261 ggactacatc ttcgaggagg tggctttcct ggcgccggcg tggctcgcgc gcgactccgc
     4321 gactgaagtg acggcggact ccccgagcgt ggatgtcatc gccaagtact acaagccaga
     4381 gggcgctgcg cgcgggcact tcttcatcgt ggccgcgaac caaagcctcg atccggttca
     4441 ggtgagcttc accgtgccac tcatgggtaa gcagaagtcc gctcgcgtgc tggtattgcg
     4501 cgagaaccgg gtgatccccg tgcgcgacgg caagttcacg gatgagctgg agggccttgg
     4561 cgcgcgaatc tacaccacgc tggaggtgct tccagacttc cgattcctgg atgagatcga
     4621 ggatgagatt gctacggcga tagagcgccc ggcgctcgcc gggaacatcc tcgccgaggg
     4681 cgcggtgaag tggaccatcg gcgattcgga aggaaccttc agttgcgata gcgatctggc
     4741 ggatgcagtt tccagcgccg ccgggtggat cccggtctat ggagaccgct cgcagtgcgt
     4801 tatccgcttc gaaaagccgg tcgtcttctc tcgtgtcgag ttttgctcgc ccaccatcgc
     4861 cagcgccgat ctcgatatct ggcaagacaa cacctggaag acgatccacc agtggaagga
     4921 tgagtacggt cgccgtctgg agtacaaggg gaagcgcgtg aggaccgacc gcattcgcat
     4981 tcggccgacc gccgcgcgtc agggcttcgg aacgagcgcc ctctacgaga tcaccgagtt
     5041 gggcctctac cggtgagctg cccgggcccg gcccgattga accctggggc tttctcctgc
     5101 gtccgtatag gtaggcatgg ggtgtgcagc gcgttgacgg gtgccctcga tcctgatagg
     5161 atagggcgga ggagcaggac gatgacgaca atacgagctt ccgcaaggcg tccgggtctc
     5221 gcctcagcgc tggtgctctt cgttgcgtgc gcgctgctgg ccttcctcgc gtctcccccc
     5281 gcgctggcgg gtggcctcaa cgacttcttc aagaagggta gcaaggagtc gcagagtagc
     5341 agcaagtcgt cggagagcgc gtccggcaag aaccgccccg agtcgggcac cgcgcgggag
     5401 aaaaagagcc ccttttccga tagtgatacc gacatctggg ggaagccggg gaagcgggag
     5461 acaggcacgc cggggaagag tggcgggctc ctcgatcttt tcaagaagag cgggaaaccg
     5521 cagccggcgc cgcatggcag ccagccggac ggatcactgc ctccggtgag ctatgagcgc
     5581 gtgcaacccg gatcgggtgg gctgatcgat ctctttcgaa agccggggcg cccgcagccg
     5641 gcaccgagca cgtctacttc gcccccgccc gcggtggtga tctaccacta ctacgacccg
     5701 tggttctacc cctacgatcg cttcttctac cgctactatg gcccctacat catccgcgag
     5761 cgggagtact actttggggg atggccgtat cactacgagc gacccgatcc ccaggaagag
     5821 gcggaggaga gcgccaacga agccctgcgc aacatcgagc ggggctggcg ggagagccgc
     5881 tacgccctga tcgcgcgcca tgtggatgat gacgagagca tcgctgtctt ccatgatggc
     5941 gactatagcc atgcgatgac ggctcgcgag ttccgcgcgc tcacgctgca ggcgtttgag
     6001 gatgtgcaga ccgttcgctt ccgctttttc gaaacgcgga tggccggcac cacccagtgc
     6061 atggccgatg gggagcacgt cttcatcggc ccggacggca ctgagcgccg tgtcgagctt
     6121 cagtacatgc tccgcaagaa ggggcgacgg tggtacatcc actctatcga tttgcgccag
     6181 gagcggcttc tctcctcgtt gcgtgctcca gagcaggtgt tgcccttgct ttcgctcgcg
     6241 tctttcaagc cggcggtgga tctggatgaa gagacgaccg aggcgcgcgc ctacactggc
     6301 ctggaagagg agctgctggg gccgccggcg attcccgcac cctccgggcc gcgggtgacc
     6361 ctggcaagca tcaccagcca agtagctacc gatgaagcga cgccctatcc ggctgccatc
     6421 gtggtcgcct ccgccgcgcc ggtcgctgtg cgccggccgg tcgccgagag cgcgatccgc
     6481 atcacgtgcg agagcaagcc ggtccgaatg gcagctgtat ctggcgctcg aaacccggtg
     6541 aaggtcgcga ccgtgacgtg cgtggtggtc caggccgcgc cggggagcac cctcgttccc
     6601 ggctcgcgcc tgacgtgtga tgtgaacgtt cgctccacga aggatggtct cgcctggagc
     6661 gtgcttcacc ggggagagac gcgcactctc gaggcgggca ccattccgtg ggcgcagctc
     6721 cagcctgccc ggacaggcca cctgcgcgtg gtggtcgacc gtgtggccac gcccggtact
     6781 gcggctaccc caggcgagca gcggcacact ctctacagga agagcgattg ggactaccag
     6841 gtggccctcg tcgcctcccc gaagccggct tcaagccaaa ccgcgcgcac cgaactcctc
     6901 gtcgccccga agacgcgcta gccctcttgt tacgacctcg ccatgcgggg gacgcctgca
     6961 acgccagtgc ttcctccttc cgggttcgcg caccaggcag acattcaggt ttgaccgggg
     7021 gcgcgatggc gcataatgta gtgggggaag gaacacaaac cgcaagagct ggatcccgga
     7081 gagcatggag gggccgtgag ggcacgaggg agcgggcgaa ggcgatcacg gcggatggtc
     7141 ggctggcctg cgccggttcg cccggcgcga cgccatgagg agctggtgtt ggtgagccac
     7201 gccaccttca tcatcggcac gacaacggcg ctgctggcag gcctcttgct cggattggtc
     7261 tggggtgccc gcctgaatca ggaacggcgt agcgtgcgcg cccggcctgg catcgcggca
     7321 gctcccttga cgagggatgt gcagccgggt ataatctgga catgctcgca aggaactgca
     7381 cgcgggacgt gatcatcgcc gatgacgttc gcgtcgcgcg ctcctggtgg aaccgttttt
     7441 gcggcctgat gctggtgccg gcgctcgctg ccgggaaggg cctcttgatc gagcgctgcc
     7501 gttcgatcca cacccacttc atgcgctttc ccatcgatgc cctcttcctc gaccgtgacg
     7561 gcgttgtggt ggctgcagtg gagaggatgc gcccatggcg catcggccgc tactaccccc
     7621 gagcgtgcgc ggtgctggag ttgcccgcgg gcacgattgc ttcgacagaa accaggccag
     7681 gcgaccgcgt ctgcttcgag gatggaggcg ggcgtgtggt ttagaagcgc ctcggagcgg
     7741 gtataccgga aacggtgcgg cgaaggcatg ccgaacactc gaggagggtt ccgatgagcc
     7801 aagtcgacct gctgatcaac gtgacccggg aggcgggagg gatccctgtg atccggttgg
     7861 agggcgaggt ggatatctac acctgtcctc aactcaagga gaccatcctg cgcctgatcg
     7921 atgacgggga ataccacatc atcatcgaca tggagaaagt cccctacatc gatagtgccg
     7981 gattgggggt gttggtggga gcgcgccggc gcgtcggaga gcacgacggc tcaatcagta
     8041 tcgtcaatcc gaatccctcc gtgtataagg ttctggagat cacgcgcctg accaagattt
     8101 tcgaagtgta ccaagacaag gagggggcgt tgacagccct cggcaggtaa tcctcccaag
     8161 cgatggcggt gagcggcggc ggcaagggcg gcaaacccaa gcctctatcc ccttgtgcca
     8221 tgccggcggc cgctcactat gcccccacag aaggaaccat gcgcctttct cacctcatca
     8281 ctctctgcaa tcggatcgct cccgagacgt tggccgagga gtgggatcat gtgggcctgc
     8341 agtgcggtga tccggaggcg gaggtctcca agctgctcct ttgcctcgat ctcaccgatg
     8401 cggttctcgc cgaagcgcgc gccctggacg cggaatgtat cctctcgcac cacccgcttc
     8461 tttttcggcc ggttttctcg ctccgcgagg atcgctatcc ggcgcgcctc ctggccggcc
     8521 tgatccgctc gggcatcgcc gcctacgcca tgcacacgaa cctcgattca acgcgcccgg
     8581 gcaccagcga tgccctcgcc gaagctctgg ggatcgtcga aacggagcca ctcgtgcccg
     8641 cggctcgccc cgccgaaacc gcgcactaca agctggccgt ctttgtgccc gccgccgatg
     8701 ccgaccgcgt tcggaaggcc ctaggtgacg ccggcgcggg tgctttgggc aactactcgc
     8761 attgcagctt cagcgcgcgc gggaccgggt cgttccgccc gctggaggga gctcatcccg
     8821 ccatcgggag tatcggcacg caggagtatg tgccggagga gaaggtcgaa gtgatcgtcc
     8881 cgaaagggat cctcccccgc gtgctcgacg cgatgctggc ggcgcaccct tatgaagagg
     8941 tggcctacga tctccttccg ctggtgccgc cacctaccgg cgccggcctg ggccgcttcg
     9001 gtcggatgga aacgccttgc accgtcgagg agctggccga acgcgcccgg gcgtgcctgc
     9061 ccacgtctcg cgtggccctc gcaggagacc ccggccgccg cgtggagcga gtcgccgtgt
     9121 gcggcggcgc tggtggcgac ctggtggatg ccgccgcagc cgcgggcgcg gatcttctct
     9181 taaccggcga tgtgaaacac caccaggcgc atcatgccct cgaacggggt ctcgcggtga
     9241 tcgatgccgg ccattacggc accgagcgcc cggtgctgga tctcctggcg cgcctcctcc
     9301 gagagaacgc cggcgacgcg ctggaagtac atctttcgcg ggtctgcacc gatcccttca
     9361 cctgagcgac ccaggctggc accatggatg tcatcgtccg cgctgcccgt gggccggggg
     9421 ggcatatcgc gtgggcgtaa catccggcgc tgcgtgtgtc ttgcggattg tcggatgcgc
     9481 ctgttggccc gtgccggttg agcggcgcgc gatgcccctg tctccccgcc ggatagggca
     9541 tctgcgagcc ctcgaacacc gcgcgttctc gtttgactca acgatccccc tatggtatca
     9601 taaccgtcgg tattatcacc catgaggacc aactgatgag cattcttact catattacca
     9661 aattgatgtt tgatgggaac gaacgcgagg tgcgtcgtct ccagggcatc gtcgatcagg
     9721 tgaacgcgct cgaacctgaa ttgcttgcgc tgtcagacga tgagattcgc gcgcgcggac
     9781 cgatgttccg ggagcgcatc caggagtcgc tggcaaagcg tttgacgccc gaggagagag
     9841 agcagcaggg gccggagtat gtggcggccc tccaagatgt tttgactgac atgctccccg
     9901 aggtgtttgc tacggtgcgg gaagcctcgc gccgcaccct tggagagggg cgcccgccga
     9961 ccgatccacg cgctatgcgt cattttgacg tccagatcat gggtggcgtg gtcctccatc
    10021 aggggcgtat tgcggagatg aagacgggtg aggggaagac gcttgtcgcc accctcccgg
    10081 tctgcctgaa tgccctgtct ggagacggcg tccacgttgc cacggtgaac gactacctgg
    10141 caaagcgcga cgcgcgttgg aacggcccga tctaccacat gctgggtctt agcgttgccg
    10201 tgattcagtc ggtgccgcac gaatccgcct acctctacga tccggagtac gtctcggacg
    10261 atccgacgat gcaccagttg cgccccatca cgcggcgcga agcctacctg gcggatatta
    10321 cctatggcac gcatgccgag ttcgggtttg actatctgcg cgacaatctg gagtttgacg
    10381 agcagcgcct gcgtcagcgc ccactcaact tcgcgattgt ggacgaggtg gactcgatcc
    10441 tggtggacga ggcgcgcacg ccgctgatca tctctggctt cgctgaggag tcgaccgagc
    10501 tctacacgcg cgtggaccgg gtggtgcgca gcctgaccgc ggagcgcgac tacaccgtgg
    10561 acgagaagca gcgcacggca accctcaccg atgagggcgt ggcgcgggtg gagcgtggtc
    10621 tcggaatcca gaacatcgcg gatgacatgc gcgtcctggg catcgtgaac gcatccctga
    10681 aggcgcacta tgtcttccgc aaggatattg actacgtcgt ctatcagcat aaggatgagt
    10741 ccggacgccg cggcgagcaa gagatcgtca tcgttgatga gaataccgga cggttgatgt
    10801 tcggtcgccg ctactccgag ggcctgcacc aggccatcga ggccaaggag aacgtgcgcg
    10861 tccagcacga gagccagacg atcgccacga tcacctacca gaactacttc cgtatgtacc
    10921 gcaagctggc cggtatgacc ggtacggcga agacggaaga gggcgagttc cggaagatct
    10981 acaacctcga cgtggtccag atcccgacca accgcccgat gatccgcatc gatcaccccg
    11041 atgtggtcta caagacggaa gaggcgaagt tccggggctg cgtggccgat atcctgcagt
    11101 gcttctgccg caagcagccg gtgctcgtcg gaacgcgcac catcgaggtc tcggagcgcc
    11161 tggccgaccg cctgtcggcg gacaatctcc agctcatggc gatgatctgg ctcctgcgcg
    11221 atcgcgtcta cggcgacaag gcgctgtcca aggaagagaa agccgaaaag catgccttcc
    11281 tcaaccggaa gatcctctac tccgcggacc gcgcgcgtct cactccggaa gagcggcgcg
    11341 agcgcttcga gtcggtgcgc gatctctggc cgatggcgaa ggagcttggc gtgtcgaccg
    11401 acgttctctc agaggagaac gtggaccggc tcgcggcgat cctagaggtc cagaacaagc
    11461 gacgcctgca gatggcgctg acctacggca ttccgcactc ggtcttgaac gcgaagtacc
    11521 acgagaagga ggcgcagatc atcgccgagg caggccgggc cggcgccgtg accatcgcga
    11581 ccaacatggc cgggcgcggt gtggatatca tcctgggcgg cacgccggac ccgaacgccg
    11641 atggctctgg ccggctcctg ccgacggata tccgggatat gacggcgctt gccaagaagc
    11701 tgcacgcggc ccgcgacccg ctgacgcgct atcttcaggg tcggctgagc gcggagacgc
    11761 ggcagctgat ggagatcgac ctctacgaag gcgcgatggc ggcctcggag aagctggagg
    11821 cagcgctggt ggaggagctc aatcgggtga tcctggggcc ttccatctat gaccctcagc
    11881 gcttccagca tctgatgctc tcggatgagg cacgcgaaat ggtggcgcac gacgggccgc
    11941 tatcgcccga gcagacggcc tggctgaacc gccggctcct ggaaagcgcc ttcccgcggg
    12001 agattgcgcg ggcctactcg cctgcggagg ctgctctcgt ccgcgaggta ggcggcctgc
    12061 acatcctggg taccgagcgc catgaagcgc ggcgcatcga taaccagctc cgcggtcgcg
    12121 cgggtcgcca gggagacccc gggtcgagcc gcttctatct ctcgctcgaa gatgagctgt
    12181 ggcgcctctt cggcgaccgt gggcatgccc tgcttggatc gtggccggag gaagagccgg
    12241 tcgaggcgaa gttgctgacc aaggcgattg cgcgcgcgca gaagaaagtg gaggagcgca
    12301 acttcggaat ccgcgagcac accctgaagt acgacgatgt gatgaacgag cagcgccgcg
    12361 ttatctacga gcagcgccgg cgcatcctgc tcggcggccg cgtttggaac ggcgttcact
    12421 acccgccggt cgatttgcgc gccaacatca tggagtcggc acaagagctg atcgtggatg
    12481 cagtcaacac gcactgcccg ccggaagtgg ctcccaacga gtgggatatc ccggggctct
    12541 accgatcgct ccacgatatc ttcgaggtgt cgcggttcct gcatgagagt gatctctacg
    12601 ggaaggagcc gaacgagctg attgagctgc tcgtgcagac ggccgagcgc gtctatgccg
    12661 agcgcgagca ggtcttcacg cccgagatcg tgcgcgagct ggagcgcaac atcttcttgc
    12721 acgtagtcaa cgagaagtgg gtggcgcacc tcgacgcaat ggattacctg cgtgagggta
    12781 ttcacctgcg cgcctatgcc caggtggatc cgctggtcgc ctacaccaag gaagcctacg
    12841 agatgtggca gg
//
LOCUS       DAOSCV010000012        12543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3386_length_12543_cov_3.687060, whole genome shotgun sequence.
ACCESSION   DAOSCV010000012 DAOSCV010000000
VERSION     DAOSCV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12543
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3386_length_12543_cov_3.687060"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2312)
                     /locus_tag="PLY56_00670"
     CDS             complement(<1..2312)
                     /locus_tag="PLY56_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOJ20021.1"
                     /translation="MSDLLESGGGISAPPEEKGAVGKRVAKATGAIVFGQMAAKILGA
                     VEKPLMALRFGTGAAADAFGAAVGLVLSLTMFVEQLLQHAFMPTFILVRTRDGEKESW
                     RLASTIVCLIVLAFATLGVVTFLFAPQVVGFATKFRNPQATQITIHLLRIMVPALIIL
                     PVSSLTYVILNSYKIFGLPAAADVAFKSGTVVALIFLAAKMGIPALALGVVLGTVVKL
                     GLHLLGLRAPLRFFRPSVDLGNPSVRRVGVLAVPLLLGSVLSFFRPLWDNWLASGLAA
                     GGAVAALRYGRAVVDTPAQIFPLALRRALFPFMSEMAARNERDRMTEMVVRSLRVVAV
                     VFIPMTLGLILLRYPIVETIYQWGKFDKESTALVVPVVTFLALGLLAMGAEMILLEPY
                     FAMSDTVTPTKIAVVTFFVHIAFTGAAVLWLGWGVGGIALGYTVARTTKVAILAWGLR
                     GRLSPVPVKPLLDFSARLTGAVLGMTAVVWITMAYPVLPREKLLIERQQLKDKSRLVA
                     AMADPNDARAPLSAHLLAGMPEESRRDLAEFYASIDPPRELKEALAADLNRILQAGPL
                     YDDARFADASLEEELWDAITANPTGDELVRVNRRLLDETYANQLPPMRDGAGPLYRPE
                     DLDHRARFIATLKQPEDPVSRYIRDSLSPETLRLAEAYPAVFEPSRELRNAIVAQLNQ
                     VLQGECIYDEELFAGIHLSSETRKLMARQPRGTTLLWLNRLLLEEAYRDEIAPPSKWV
                     DLCRLSVPSAVGAFVFVLLCMLLRVEEATYL"
     gene            complement(2309..3589)
                     /locus_tag="PLY56_00675"
     CDS             complement(2309..3589)
                     /locus_tag="PLY56_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ20022.1"
                     /translation="MDELVYIVGEFPSLSETFILREMLDLVDRGVPLRIYALKRPRSG
                     RRHADCARLMERVTYRPRRGSIQWLAGHAAMALARPRGYTRLLARALREAGSSRYQLR
                     ERLVAFDAAVGLARAPFASRIRHLHGHFAGVPATTARYVAELLGGGQKSPAPRYSFTA
                     HAQDIYVGGQKDRAGLEANLRGAAFVVTCTGYNREHLIREHPGVPAEKIHRVYHGIDT
                     RRFAPAKERGTGPAQILAVGRLLPKKGLHHLVAACGALRERGVAFQLIVAGDGPERDR
                     LERLAAGHGIAGNTTFLGEVVQEELVAVYAQADLFALPCVVTPEGDRDGLPNVILEAM
                     ASALPVVSTRVSAIPEAVEDGVTGLLVPPGDVDALAAALEKLIRDAGLRQRLGEAGRA
                     RVERDFDIWNSPLAELFARECDAVAHAIAGKETR"
     gene            complement(3582..4712)
                     /locus_tag="PLY56_00680"
     CDS             complement(3582..4712)
                     /locus_tag="PLY56_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOJ20023.1"
                     /translation="MRIGIDGLLLWGHYSGVERSIARMVTTLAQLDQTNRYTIHVPRD
                     CEAEELAAAGVEVRRAPFRGAQKLTRILWQQGVFPGVLACEGVDLLFAPGYVLPLRWR
                     GPSVLFVYDIIAITRPDLASRANALHYGALLPRSVRRATRVAVPTEHVRRQVIERCGA
                     RPEQVAVVPLGVSPHFRRVEDPARQEEVRRRYRLPERFVLTLGNLEPKKNLPRVLEAY
                     EILRREGIRHGLVIAGQPAWGARVLRENLARCAWRADIVLPGYVADADLPVLYSMADA
                     LVFPSLTEGFGLPPLEAMACGTPVVVADVPPFDETLGDAAARVAPEDAWAIATGIRQV
                     LEDPLLAGRLREAGRERAALFTWERCASLLAKLFDSAVTAHG"
     gene            complement(4781..6049)
                     /locus_tag="PLY56_00685"
     CDS             complement(4781..6049)
                     /locus_tag="PLY56_00685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ20024.1"
                     /translation="MGKGLGARAGREAPFSPDASPTAGRGHDAVGPSRAPSILLVNHV
                     SLVSGAERSLLGLARRLPDEGFSIEAALPPGGPLCDELAALGIPVYPVRLARLKRRTS
                     PLRHLHAGIRLGASAHSLARLARRREIAIVHANSTTAALAALIAARRARLPGIWHVRD
                     LVPLGGLGRWLGRLATRVIAISSAVADQVASWGVARERIVTLPNGIDPEAFRPDEDAR
                     MRLRSELGIPPEAFVVAAVGQIVPWKSHHVLLDAVGRLRRAPEGVEIRCLIAGADLFG
                     DHPDYEALLRARAMQPDLRGAVTFLGHRTDVTAVYNACDAFALPSRREPFGRALVEAM
                     ACGKPCIACNEAGPAEIVADGETGLLVPPGDAGALAVAIDRLRRDAALAACLGEAGRA
                     RALAEYTEATTAARTASLYRELLGMEEEGA"
     gene            complement(6054..7820)
                     /locus_tag="PLY56_00690"
     CDS             complement(6054..7820)
                     /locus_tag="PLY56_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ20025.1"
                     /translation="MPEEAVILAGEGPGLFGVLHRPAGKPTLALVFCPPFAEEKKGAH
                     RLLVETARALAGQGVAVLRFDYRGTGDSDGSFTDFTLADAEEDIRRALAFVRAQYPGT
                     PAGLLGVRLGAALAARVAESWPSEAGRAHCLMLWEPVTNGRRYAQMNLRQKRIREMLT
                     AKEGAEATGQAQPEAPKPKSTAPAAEGYDFDGYWVTPELHAQMEALVFPGEIRQPPSH
                     VLGISVNAAGRVSRELEAVLAPYATAGAGVDTRAIVAEPFWSLQDPVPVPDLVQATVA
                     WISDLLAPDCSGEATASATPATATRELSLPASQERPVRFQCNGRWVHGVLSLPKDPAR
                     PTTGLVTLHGWSGTRIGPHALFVSLARRAAEAGIACLRFDFRGRGDSEGEIAEATRAT
                     MIEDAVAAARFLREEGCERVILVGLCAGSQVAIGAATQGADPDGLILWSAPVSEQAEE
                     ERAVAAKRRHFLREYARKLFRPQTWRKLIGGKLQFGKIARVLSGKAGIAATDDRAVDL
                     QAAARLKEFRGPIFFVYGTHDPIAPVALPYYQRILASGQAEVSVTQIEGANHSFYGTA
                     WREEVLEQTLAWLARHFPSGGG"
     gene            complement(7823..8089)
                     /locus_tag="PLY56_00695"
     CDS             complement(7823..8089)
                     /locus_tag="PLY56_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ20026.1"
                     /translation="MAERQSIESQIKELMVERLFLKVSPEEIGDEEVLMDAYGLDSVN
                     LFELVAGLEEVYDITFEDDDFDLELFKNVASIAAYVRRKLGQEG"
     gene            complement(8104..8937)
                     /locus_tag="PLY56_00700"
     CDS             complement(8104..8937)
                     /locus_tag="PLY56_00700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ20027.1"
                     /translation="MAEASAVVLSYNKREYTRRCLQSLVQTDYPGLELVVVDQGSTDG
                     TREWLETFVPEAAGRGIPVRVVQNDHNVGACTGRNQGIAAASGRLLAFLDNDVVVEDS
                     GWLRALAGRLDSEAGAGLITPKLLYPVPPHLIQYAGCAVTPTGRIVYVGRGEARDDPR
                     FNEERELQCTISACIVVKREVFDRVGTFDEAFNPVQYEDLDLSYRAREAGYRVIYYPK
                     VEAFHYENVTTDGSPDVNFKYVTIKNGMLFKRRWRHMFSVEGGPEESEAVWKPIERPR
                     I"
     gene            complement(9119..10087)
                     /locus_tag="PLY56_00705"
     CDS             complement(9119..10087)
                     /locus_tag="PLY56_00705"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOJ20028.1"
                     /translation="MYFTEALREALAEEMRRDPNVFLMGEDMGVYGGCFGVTRGLLEE
                     FGPDRVRDTPISEASIEGLAVGAAILGARPVVEVMFMDFVTLAMDQLVNHAAKFHYMY
                     GEQARVPLVLRCPAGAGRAYGATHSQSLEAWFMHVPGLRVVAPSTAADAKGLLKSAIR
                     DDNPVIFLENKLLYQSREPVPEHNYLIPLGHARCVREGTDVTIVAYSRMAREAEKAAA
                     ALAEEGISAEVIDLRTLVPLDIVTVVNSVVKTGRVILAEEGTLTGGVGAELGMRIFES
                     AWDSLDAPIRRVAMPDIPIPFAPELEAAVLPSAVDIVLAARELVDI"
     gene            complement(10138..11082)
                     /locus_tag="PLY56_00710"
     CDS             complement(10138..11082)
                     /locus_tag="PLY56_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOJ20029.1"
                     /translation="MEPRECYRRMWIIRLFEQKVDQLFGEGELRGTSHLCVGQEASAV
                     GACAALRPDDYATSTHRGHGHFLAKGGDPRRLMAELFGKETGYSRGRGGSQHMACYAI
                     GFLGSNGITGGGIPIATGAALAVQRRGGDQVVVCFFGDGASNQGVFHESLNMASLWKL
                     PVIYLCENNLYAMSTPVQKAISVPNVADRAAAYNIPGVVVDGNDFFAVCAAVDEAAAR
                     ARRGEGPTLIECKTYRMVGHSKSDRRVYRTREEEAEWQARDPIPRFRDALLAAGHLTP
                     EEAERIPDEAAREIEESVEFAKRSPVLPIEAALEGVYA"
     gene            11560..>12543
                     /locus_tag="PLY56_00715"
     CDS             11560..>12543
                     /locus_tag="PLY56_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOJ20030.1"
                     /translation="MSLSLRRALLGRPIATAHAAQQRLPKWIALPVFASDAISSTAYA
                     TEEILIALLLAGTAALSASLPIAAAIVLLLAIVTFSYQQTMLAYPGGGGAYVVARENL
                     GFGPATVAGAALLIGYVVTVSVSVAAGVHAITSAFAALRPYSVQIGVLFIALLALANL
                     RGLREAGLLFALPSYAFMFAMFLTIAVGVARLAMGAISPLEYPAEVRTETVSVTGLLW
                     VFYLLRAYASGCTALTGIEAIANGVPAFRPPEGVNAAKTMAMMGGILVVIFLGITYLA
                     HALQVVPDVGGLEHGSGETVLSKIAELVWGGKRGFYYFVQATTMLILVLAAN"
BASE COUNT         1986 a         4491 c         4088 g         1978 t
ORIGIN      
        1 agataggtcg cctcctccac gcgcaggagc atgcacagga gcacaaagac gaacgcgccc
       61 accgcgctgg gcaccgagag ccggcagaga tccacccact tgctcggggg tgcgatctca
      121 tcgcgatacg cctcctccag caggaggcga ttcagccaca ggagcgtggt gccccgtggc
      181 tggcgcgcca tcagcttccg cgtttccgac gaaaggtgga tccccgcgaa gagctcctcg
      241 tcatagatgc actcgccctg gagcacctgg ttcagctggg cgacaatcgc gttacgcagc
      301 tcgcgcgagg gctcgaaaac ggccggatac gcctctgcca ggcgcagtgt ctccggcgac
      361 agcgagtcgc gaatgtagcg cgataccgga tcctccggct gcttgagcgt cgcgatgaag
      421 cgcgcgcggt ggtctaggtc ctccgggcgg tagagggggc ccgccccatc gcgcatcggc
      481 gggagttgat tggcgtaggt ctcatccagc aagcgccggt tgacgcgtac caactcatcg
      541 cccgtggggt tcgccgtgat cgcgtcccac agctcttctt ccaggctggc atcggcgaag
      601 cgcgcgtcgt cgtagagggg gccggcctgc aagatacggt tcaggtcggc ggcaagcgcc
      661 tctttcagct cgcgcggggg gtcaatggag gcgtagaact ccgccagatc gcgccgcgac
      721 tcttccggca tccccgcgag aaggtgcgct gaaagagggg cacgcgcgtc gttcggatcg
      781 gccatcgccg ccaccaggcg cgacttgtct ttcagctgct gccgttcgat gagcagcttc
      841 tcgcgcggga gcaccgggta ggccatcgtg atccagacga ccgccgtcat ccccagcacc
      901 gccccggtca gacgcgcgct gaaatcgagc aatggtttga ccggcaccgg ggagagccga
      961 ccgcgcaagc cccacgcgag aatcgccacc ttcgttgtgc gcgccacggt gtagcccagg
     1021 gcgatgccgc ccacacccca gccaagccac agcacggccg cccccgtgaa ggcaatgtgg
     1081 acaaagaagg tgacgacggc gatcttcgtc ggcgtcaccg tgtccgacat ggcgaagtag
     1141 ggctccagga ggatcatctc ggcgcccatc gccaggagcc ccagcgccag gaaagtgacc
     1201 accggcacca ccagcgccgt cgattccttg tcaaacttgc cccactggta gatcgtctcg
     1261 acgatcggat accggagcag gatcagcccg agcgtcatcg ggatgaagac gacggcgacg
     1321 acgcggagcg agcggaccac catctccgtc atgcggtcgc gctcgttgcg cgcggccatc
     1381 tcgctcatga aggggaagag cgcccgacgg agcgccagtg ggaagatctg agccggtgta
     1441 tcgaccacgg cgcgcccgta gcggagcgcc gcgaccgcgc cccccgcggc caggcccgac
     1501 gcgagccagt tgtcccacaa cggccggaag aaggagagca cgcttcccaa aaggagcggc
     1561 acggccagga cgcccacccg gcgcacactc gggttgccca ggtcgacgct aggccggaag
     1621 aagcggaggg gagcgcgcag gccgagcagg tgcagcccca gcttgaccac cgtgccgagg
     1681 accacgccca gcgccagcgc cggaatcccc atcttcgcgg cgaggaagat gagcgccacc
     1741 accgtgccgc tcttgaacgc cacgtcggcg gcggccggca gcccgaagat tttgtaggag
     1801 ttgaggatga cgtaggtgag cgaggagacc ggcaggatga tcagcgccgg caccatgatc
     1861 cgcaagaggt gaatcgtgat ctgagtggcc tgcgggttcc ggaacttcgt cgcgaagccc
     1921 accacctgcg gcgcgaagag gaacgtcacc acgcccaggg tggcgaatgc gaggacgatc
     1981 aggcagacga tggtgctggc cagacgccaa ctctccttct cgccgtcgcg cgtgcgcacc
     2041 aggatgaagg tgggcatgaa ggcgtgttgt aggagctgct ccacgaacat ggtgaggctg
     2101 agcaccagcc cgaccgctgc gccgaacgca tctgccgccg cacccgtgcc gaagcgcagc
     2161 gccatcaacg gcttctccac cgcgcccagg atcttcgcgg ccatctgccc gaagacgatg
     2221 gcgcccgtgg ccttggccac acgcttgcca accgctccct tctcctcggg cggggccgat
     2281 atccctcctc cactctccaa cagatcgctc accgcgtctc ctttcccgcg atagcatgcg
     2341 cgaccgcgtc acactcccga gcaaacagct ctgcgagggg gctgttccaa atgtcgaaat
     2401 cacgctccac gcgcgcccgt ccggcctctc ccaggcgctg gcgcagcccg gcgtcacgaa
     2461 tcagcttctc cagagcggct gccagcgcgt ccacgtcgcc cggtggcacc agcaggcccg
     2521 tcactccatc ttccaccgct tccggaatgg ccgagacgcg cgtgctcacc accggaagcg
     2581 cggaggccat tgcctccagg atcacgtttg gcagcccgtc gcggtctccc tccggggtga
     2641 ccacgcacgg aagcgcgaaa agatcggcct gggcgtaaac cgcgaccagc tcttcctgga
     2701 ccacttctcc caagaaagtc gtgtttcccg cgatcccgtg cccggcggcc aggcgctcca
     2761 accggtctcg ctcgggtcca tccccggcaa caatcagctg gaacgccacg ccgcgctcgc
     2821 gcagcgctcc acatgcggct accagatggt gcagcccctt cttcgggagc agccggccca
     2881 cggcgaggat ctgcgccggc ccggtgccgc gctccttcgc gggggcgaag cgccgcgtat
     2941 cgatcccgtg gtacacccga tggatctttt ccgccggcac ccccggatgc tcccggatca
     3001 ggtgctcgcg gttgtagccg gtacacgtca ccacgaacgc cgctccgcgc agattcgctt
     3061 cgagcccggc gcggtccttt tgcccaccca cgtagatatc ctgggcgtgc gccgtgaagg
     3121 agtagcgggg cgcggggctc ttctgccccc ctcccagcag ctccgccacg tagcgggccg
     3181 ttgtcgcggg cacgcccgcg aaatggccat gcaggtggcg tatccggctg gcaaagggcg
     3241 cgcgtgccag ccccaccgcc gcgtcaaacg ccaccaggcg ctcgcgcagc tggtagcgcg
     3301 agcttccggc ctcgcgcagg gcgcgcgcca ggaggcgcgt gtagcctctc ggacgggcga
     3361 gcgccatcgc cgcgtgccca gccagccatt ggatgctgcc gcgccggggc cggtaggtca
     3421 cgcgctccat gagccgggcg caatcggcgt gccttcgccc tgaccggggc cgtttcagcg
     3481 cgtagatccg caggggcacg ccgcggtcca ccaggtcgag catctccctc agaatgaacg
     3541 tctccgagag cgacgggaac tcgccaacga tgtacaccag ctcatccatg agctgtcacc
     3601 gccgaatcga acagctttgc caggagcgac gcgcaccgct cccaggtgaa cagggcggcc
     3661 cgctcccggc ctgcctctct caggcgcccg gccaggagag ggtcttccag cacctgccgg
     3721 atgccggtgg cgattgccca ggcatcctcc ggggccacgc gcgcagctgc gtcgcccagc
     3781 gtttcgtcaa acggcggcac gtcagccacg acgacgggcg tgccgcacgc catcgcctcc
     3841 agcggtggca gcccgaaccc ctctgtcagc gaggggaaga cgagcgcgtc cgccatcgag
     3901 tagagcaccg gcaggtccgc gtctgcgaca tagcccggga gcacgatatc ggcacgccag
     3961 gcgcagcgcg ccaggttttc gcggagcacg cgcgcccccc aggcgggttg gcccgcgatc
     4021 accaagccgt ggcggattcc ctcgcgcctc aggatctcat acgcttccag cacccgcggt
     4081 aggttctttt tcggctccag attccccagc gtcagcacga agcgctctgg cagccggtag
     4141 cgccggcgga cttcctcctg cctggccggg tcctcgacgc gccggaagtg cggcgagacg
     4201 cccaggggca ccacggctac ttgctcgggc ctcgcgccgc accgctcgat cacctggcgg
     4261 cggacgtgct ccgtgggcac ggcgacgcgc gtcgcccggc gcacgcttcg cggcaatagc
     4321 gcgccatagt ggagcgcgtt ggcccggctc gcgagatcgg ggcgcgtgat ggcgatgatg
     4381 tcatacacga aaagcacgct cggcccccgc cagcgcagcg gcagcacgta gccgggcgcg
     4441 aagaggagat cgacgccctc gcacgcgagc acgccgggaa agaccccctg ctgccacaga
     4501 atgcgcgtca gcttttgcgc cccccggaag ggcgcccgcc tcacctccac tcccgcggcg
     4561 gccagctctt ccgcctcgca atcgcgcggc acgtggatcg tgtaccggtt cgtctggtcg
     4621 agctgggcca gcgtggtcac catccgcgcg atggagcgct ctacgcctga atagtgcccc
     4681 cagaggagca ggccatcaat cccaattcgc attgctattg atccgcggtg atgcccgcgg
     4741 cccccggaac tcaccccgaa tgggtgagct tcccgggtga ttacgccccc tcttcctcca
     4801 tgcccaggag ctcccggtaa agcgatgccg tgcgcgccgc cgtagtggcc tcggtgtatt
     4861 cggccagcgc gcgcgcccgg ccggcttcgc ccaggcacgc tgcgagcgcc gcgtctcgcc
     4921 gcagccggtc aatcgccacg gcgagcgcgc ccgcgtcccc cggaggcacc agaagccccg
     4981 tctctccatc cgctacgatc tccgccgggc cggcctcgtt gcacgcgatg cacggctttc
     5041 cgcatgccat cgcctcgacg agcgcgcgcc cgaacggctc tcgccgcgag gggagtgcga
     5101 aggcgtcgca cgcgttgtag accgcggtca cgtcggtccg gtgccccaga aaggtgacgg
     5161 cgccgcgcag gtcgggctgc atcgcccggg cgcgcaggag cgcctcgtaa tcgggatggt
     5221 cgccgaagag gtccgcgccg gcgatgaggc agcgaatctc cacgccctcc ggcgcgcgtc
     5281 gcagtcggcc cacggcgtcg aggaggacgt ggtggctctt ccacgggacg atctgcccga
     5341 cagcggcgac tacgaacgcc tccgggggga tgccgagttc ggatcgcagg cgcattctcg
     5401 cgtcctcgtc cgggcggaac gcctccgggt cgatcccgtt gggaagggtc acgatccgct
     5461 ctcgcgcgac gccccagctt gcaacctggt cggccacggc gctggagatg gcgatcaccc
     5521 gggttgccag ccggcccagc caccggccca ggccgccgag gggcaccagg tcgcgcacgt
     5581 gccagatccc gggcaggcgc gcgcgccggg cggcgatcag ggccgcgagc gccgcggtgg
     5641 tgctgttggc atgaacgatc gcgatctcgc ggcgccgtgc cagccgggcc agggagtgcg
     5701 cgctcgcccc caggcgaatc cccgcgtgca ggtggcggag cggcgatgtc cggcgtttca
     5761 gccgggccag gcgcaccggg taaaccggga tcccaagcgc cgcgagctca tcgcagagcg
     5821 ggccgcctgg cggcaacgcc gcttcaattg agaaaccctc gtccggtagc cgccgcgcca
     5881 ggcccagcag gctccgctcc gccccgctca ccagcgagac gtggttcacc aggaggatgg
     5941 agggggcgcg ggagggaccg acggcgtcgt ggccgcgccc ggccgtggga gaggcgtccg
     6001 gcgagaaagg ggcctcgcgt cctgctcttg ctcccaggcc ctttcccatg cgtctacccg
     6061 ccccccgaag ggaagtgcct ggccagccag gcgagcgtct gctccagcac ctcttcgcgc
     6121 caggcggtgc cgtagaagga gtggttggcg ccctcgatct gcgtgacgga gacctctgcc
     6181 tgcccggacg ccagaatccg ctggtagtag gggagggcga ccggcgcgat cggatcgtgc
     6241 gtgccgtaga cgaagaagat cggcccgcgg aactccttga gccgggcggc cgcctggagg
     6301 tccaccgcgc ggtcatcagt ggcggcgatc ccggccttcc cggagaggac gcgcgcgatc
     6361 tttccaaact gcagcttgcc cccgatcagc ttgcgccacg tctgcggccg gaacagcttg
     6421 cgcgcgtact cgcgcaggaa atgccggcgc ttggccgcga cggcgcgctc ctcttccgcc
     6481 tgctccgaga ccggcgccga ccagaggatg agcccgtccg gatcggcgcc ctgggtcgcg
     6541 gcgccgatgg cgacctgcga gccggcgcac agccccacca ggatcacgcg ctcgcatccc
     6601 tcctcgcgca ggaagcgcgc cgcagccacc gcgtcctcga tcatcgtggc gcgagtggcc
     6661 tcggcgatct cgccctcgct gtcgccacgc ccgcggaaat cgaagcggag gcaggcgatc
     6721 cctgcttcgg ccgcgcgccg cgcgagcgac acgaacagcg cgtgcggtcc gatgcgcgtg
     6781 ccggaccagc cgtggagcgt caccaggccc gtcgtcgggc gcgcggggtc cttcggcagg
     6841 ctcaacacgc catgtaccca ccgcccgttg cactggaagc gaaccggtcg ctcctggctc
     6901 gcgggcagcg acagctcgcg cgtggcagtg gccggggtgg cagacgccgt ggcctcgcca
     6961 ctgcaatccg gggcgagcaa atctgagatc cacgcgaccg ttgcctgcac cagatcgggc
     7021 acgggcaccg ggtcttgcag gctccagaac ggctcggcga cgatggcacg cgtgtcgact
     7081 ccggctccgg cggtggcgta gggtgccagg accgcctcca gctcgcgcga gacgcggccg
     7141 gcggcgttca cgctgattcc gagcacgtgc gacggcggct ggcggatctc gcccgggaag
     7201 acgagcgcct ccatctgcgc gtgcagctcg ggcgttaccc agtagccgtc gaaatcatag
     7261 ccctccgcgg caggagcggt ggattttggt ttgggcgctt ccggttgcgc ctggccagtg
     7321 gcctccgcgc cctctttcgc cgtcagcatc tcgcggatgc gcttctggcg caggttcatc
     7381 tgggcatagc gccgcccgtt cgtcaccggc tcccacagca tcaggcagtg tgcgcgcccc
     7441 gcctcggacg gccacgattc cgcgacgcgc gcagccagcg ccgcccccag gcgcacgccc
     7501 agaagcccgg caggcgttcc gggatactgc gcgcgcacga aggcgagggc cctccggata
     7561 tcctcctccg cgtcggcgag cgtgaagtcg gtgaacgagc catcgctatc gccggtgccg
     7621 cggtagtcga agcggagcac ggccaccccc tggccggcca gcgcgcgcgc cgtctcgacg
     7681 aggaggcggt gcgccccctt cttctcttcc gcgaatggcg ggcagaagac gagcgcgagc
     7741 gtaggcttcc ccgccggtcg gtggagtacc ccgaaaaggc caggaccctc gcctgccagt
     7801 atgaccgcct cttcaggcac gcctatccct cttggcccag cttgcggcgg acgtacgccg
     7861 cgatcgaggc cacgttcttg aacagctcca ggtcgaagtc gtcatcctcg aaggtgatgt
     7921 cgtacacttc ctccagcccg gccaccaact cgaagaggtt caccgagtcc aggccatagg
     7981 catccatcaa cacctcttcg tcgccgatct cctccggcga caccttcagg aagagccgtt
     8041 cgaccatcag ctccttgatc tgcgattcga tgctctgtcg ttccgccata tcctcccgct
     8101 tttctagatc cgcggccgct caatcggctt ccacaccgct tcgctctcct cgggcccgcc
     8161 ttcgacggag aacatgtgcc gccagcgccg cttgaagagc atgccgttct tgatcgtcac
     8221 gtatttgaag ttcacatcgg gcgagccatc tgtggtcaca ttctcgtaat ggaacgcctc
     8281 caccttcgga taatagatca cgcgataccc ggcctcgcgc gcccggtagg agaggtcgag
     8341 atcctcgtac tgaaccgggt tgaacgcctc gtcgaacgtt cccacgcggt cgaacacctc
     8401 gcgcttcacc acgatgcagg cgctgatggt gcattgcagc tcgcgctcct cgttgaagcg
     8461 cgggtcatcg cgcgcctcgc cccggcccac gtagacgatc ctgcccgtgg gcgtcaccgc
     8521 gcatccggca tactggatca ggtgcggcgg caccggatag agaagcttcg gcgtgatcag
     8581 gcccgcgccc gcctcggagt ccaggcggcc ggcgagggcg cgcagccacc ccgaatcctc
     8641 gaccaccacg tcattatcaa ggaacgctag gagccgcccc gaggcggcag cgattccctg
     8701 gttgcgcccg gtacacgcgc cgacgttgtg gtcattctgc accacgcgca ccgggatccc
     8761 gcgcccggcg gcttctggga caaacgtctc cagccattcg cgcgtgccgt cggtggagcc
     8821 ctgatcgacg accaccagct ccaggcccgg atagtcggtc tgcaccaggc tttgcaggca
     8881 ccgtcgggta tactcccgct tgttgtagct cagcacgacg gcagatgctt cagccatgac
     8941 agcgctccgg gggcgagcgg ccccggcccc cggcctcctc tcacatttcg ttccgcggtc
     9001 ctatcgtctc gcgcgcgtca ccccgccgcg ggtgcgccct gaaaaccgga tgtgttgggc
     9061 ttgcagggga ggccccgtcg gccctgctgc ctgggcgcca ggctatccgc cggagggact
     9121 agatgtctac cagctcccga gccgccaaga cgatatcgac tgccgacggg agaaccgcgg
     9181 cctccagctc cggggcgaag gggatcggta tgtccggcat ggcgacgcgg cggatcggtg
     9241 cgtccaggga atcccaggcg ctctcgaaga tccgcatccc gagttcagcg cccacgccac
     9301 cggtgagggt tccttcctcc gccaggatca cgcgccctgt cttgacgacc gagttgacga
     9361 ccgtcacaat gtccagcggc accagcgtgc gcaggtcgat cacctcggcg gagattccct
     9421 cctcagcgag cgctgccgcc gctttttccg cctcgcgcgc catgcgcgag taggcgacga
     9481 tcgtcacgtc ggttccctcg cggacacacc gggcatgtcc cagagggatc aggtagttgt
     9541 gctcgggcac cggctcgcgc gactggtaga gcagcttgtt ctcgaggaag atcacggggt
     9601 tgtcatcgcg gatggcgctc ttgagcaggc ccttcgcgtc ggcggctgtg gaaggcgcca
     9661 ccaccctcag cccaggcacg tgcatgaacc acgcctccag gctttgcgag tgagttgccc
     9721 cgtaggcgcg gcccgcgccc gccggacagc ggaggaccag gggcacgcgc gcctgctcgc
     9781 catacatata gtggaacttc gccgcgtggt ttacgagctg gtccatggcg agggtgacga
     9841 aatccatgaa catcacttcg accacgggac gggcgccgag gatcgccgcg cccaccgcca
     9901 ggccctcgat cgacgcctcg gagatcggcg tgtcgcgcac ccggtccgga ccaaactctt
     9961 ccagcaggcc gcgggtcacc ccgaagcacc cgccgtagac gcccatatct tcccccatga
    10021 ggaagacgtt gggatcacgg cgcatctcct cggcgagcgc ctcacgcagc gcctcggtga
    10081 agtacatctc tcgcgcgggc gaagtggccg ccacgccctc gggcaccgtt gtctccttca
    10141 cgcgtacacc ccctccagag ccgcttcgat gggtagcacc gggctgcgct tcgcgaactc
    10201 cacgctctcc tcaatctctc gcgcggcctc gtcggggatc cgctctgcct cctccggggt
    10261 gagatggcca gcggccagca gggcatcgcg gaaccggggg atcgggtcgc gtgcctgcca
    10321 ttcggcctcc tcctcgcgag ttcgatacac ccgccggtcg ctcttcgagt gcccgaccat
    10381 ccggtaggtt ttgcattcga tgagcgtcgg gccctcgccc cggcgcgcgc gcgccgcggc
    10441 ctcgtctacc gccgcgcaca ccgcgaagaa atcgttgcca tccaccacga ctccggggat
    10501 gttgtaggcc gccgcgcgat cggccacgtt cggcaccgag atcgccttct gcaccggcgt
    10561 ggacatggcg taaaggttgt tctcgcacag gtagatgacg gggagcttcc agagcgaggc
    10621 catgttcagc gattcatgga agacgccttg gttagaggca ccatcgccga agaagcagac
    10681 gaccacctgg tcgcccccgc ggcgctggac ggcgagcgcg gcgccggtgg cgataggaat
    10741 cccaccgccg gtgatgccgt tcgagccgag gaagccgatg gcgtagcacg ccatgtgctg
    10801 gctacccccc cgaccgcgcg aatagccggt ctccttgccg aacagctccg ccatcagccg
    10861 gcgcggatcg ccccccttgg ccaggaagtg cccatgcccg cggtgcgtgc tcgtcgcgta
    10921 atcatccggg cgcagagccg cgcacgcgcc gacggcggag gcctcctggc caacgcagag
    10981 gtgcgatgta cctcgcagct cgccctcgcc gaacaactga tctacttttt gctcgaagag
    11041 acggatgatc cacatccgcc ggtagcattc gcgtggttcc atggcttctc gttagacccc
    11101 cgttcggcgg cttaagtttc gagagagcgg caccccaggg gggcccggca gagcggggag
    11161 ggggacagag caccgccaca tcggcattct cccacccgcg tgcatctcct tcatccgcaa
    11221 ttccacagat gaaggagacg ctcgcggatg ggccggcggg gagtccgggg gcggtgcctc
    11281 cggcgacggc tggcgcttgg cccggcgcac tcaccctaga gggagacggc accatccgcg
    11341 tccgccccct tccgtggtat cggactccgt cttgcggcag ttcccccttt gtgctatact
    11401 ctgccaatgt cagatggcga gcctccttgt ggatgcatgc catgagcgct cacctatcga
    11461 ttgcgtgagc tgacctgtgg tctccgagac gcccgtaaca tgctattgcg ggcgcttacc
    11521 tgctgcccgg ccagctcctg atgatggaga aaccggaaga tgtccctatc gcttcggcgt
    11581 gcgcttttgg gccgcccgat tgccacggca cacgcggcgc agcagcggct cccaaaatgg
    11641 atcgcgctcc ctgtctttgc ctcggatgcc atctcttcga ccgcctacgc cacggaagag
    11701 atcctgatcg cgttgctcct cgccggcacg gcggcgctct cggcgtcgct gccgattgcc
    11761 gccgcgattg tccttctcct ggcgattgtg accttctcct accagcagac gatgcttgcc
    11821 tacccggggg gtggcggcgc ctacgtcgtt gctcgcgaga acctgggctt tggccccgcc
    11881 acggtagccg gcgccgcgct cctcatcggc tacgtcgtca ccgtctcggt cagcgtggcc
    11941 gcgggcgtgc acgccatcac ctcggcgttc gccgcgctcc ggccctattc ggtgcagatc
    12001 ggcgtgcttt tcatcgcctt gcttgctctg gcgaacctgc gcgggctgcg cgaggccggg
    12061 ctgcttttcg cgctcccctc ctatgccttc atgtttgcca tgttcctcac catcgcggtg
    12121 ggcgtggccc ggctggcgat gggtgcgatc tccccactgg agtatcccgc cgaagtgagg
    12181 accgagactg tctcggtgac cggcctcctc tgggtgttct acttgttgcg cgcgtatgct
    12241 tccgggtgca cggcgctcac cggaatcgag gccatcgcca atggagtgcc ggcattccgc
    12301 ccgccggagg gagtcaacgc cgcgaagacg atggcgatga tgggcggaat cctggtggtc
    12361 atcttcctgg gcatcaccta cctggcccac gccctgcagg tcgttcccga tgtaggcggg
    12421 ctggagcacg ggagcggcga gacggtgctc agcaagattg ccgagttggt gtgggggggc
    12481 aagcgaggct tctactactt cgtgcaggcg accacgatgc tcatcctggt cctggctgcc
    12541 aac
//
LOCUS       DAOSCV010000013        12118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3531_length_12118_cov_3.837105, whole genome shotgun sequence.
ACCESSION   DAOSCV010000013 DAOSCV010000000
VERSION     DAOSCV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 12118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12118
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3531_length_12118_cov_3.837105"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..610)
                     /locus_tag="PLY56_00720"
     CDS             complement(20..610)
                     /locus_tag="PLY56_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20031.1"
                     /translation="MMVLRRWQELYARAESASKTFFSEHFGDLCARCAEAAETAADPS
                     ICCCRRTNFVPEILTDPLLGELADESLGHSLYALSGPHNRRGCAALGPAGCLLPFGRP
                     DPCHAYICDALYDSLDAILPLDLLCRIRDALEVYSRIREAPRRDDSEFQGCVAQVAEL
                     EALFSLASEQIWAQSAAFALRKEEALREMLPPAPIG"
     gene            complement(607..816)
                     /locus_tag="PLY56_00725"
     CDS             complement(607..816)
                     /locus_tag="PLY56_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20032.1"
                     /translation="MQLREEELELATRRSVIARVLRTRFDAGAIAEEAYRCLLGRIEG
                     ARTREALDAVLAEVEKASAGAPGSG"
     gene            complement(849..3350)
                     /locus_tag="PLY56_00730"
     CDS             complement(849..3350)
                     /locus_tag="PLY56_00730"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOJ20033.1"
                     /translation="MILLVLLAGLGPAHAAETVPVPVTLVDLREADASTKLALACLQG
                     IVNRGAERPVLYCVHTAQDRAWFDQVYAARGFKPEEKTPAEAIARFADRIKGEVLYDP
                     AQPETINLATALAGLEDVLPVTAAARDASRKLVEDLTRFKDRREACHWAIESLLPRCN
                     RAKLAVLKPEQVGLRDYLVAEKILPVGLNPGVKEEAELLRTLLGKLEPGALLFWGERI
                     GSPVETRALFARDPGDAMPALARATAMRLVPGADVANLSFHARVPAYAPLRQYKRLVE
                     HHLASYVTFLFSGNGNLDFGLGRMSTLWDDAARGYIPLGWELHPLLAELAPALLSYYY
                     VDAWWAGVDQFVMAPSGASYLPPADPKAFEAFVAETNEAASRADLTAVSVIDVSASDH
                     DRPWARLALQPAVRGFLAAGTSLGPTLSLGKPLIGETVHVTDVEKATEAILAAAQKQR
                     FVLAVVDAEKVTPTDVVQIAARLGRGIRVVTPHYFLDLLAERVFLDTVGPTMALAKLT
                     KVTFSPREPGPNDPITVKATISGGVIDARVKYNINGSPDFTEPLAKVGDDYVATIPPT
                     LRGGKLTFKVRITDGLFRTSWSGECALDIKATDSDEDGLSDYHEELCGLDPKNKDTDG
                     DGLFDANDSAPLVPFPRPKDVEGPIITAIVTAPRTPPAAGKPMALRALVFDPTGVKEV
                     TVKVAAGERDLGRGEMKQIGSTQVYEYPLPTLVAGQTVTFTIGAVNGAGKASLVTESM
                     PVTVGIRITSPPANTLIQKPTPIRIQTAGQVALVRVLINGFEVARLSKPPYEYVWNPA
                     QYDAATHEVMVEAFDGEGMLLDADAVYLRVPPKRR"
     gene            3626..4726
                     /locus_tag="PLY56_00735"
     CDS             3626..4726
                     /locus_tag="PLY56_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ20034.1"
                     /translation="MTSQVLFASAHVTRLDRDATLPAKFERLLEKADLAPRFGGKRVA
                     IKMHVGSGIGFYTIHPLFVRQVVDAVKKAGGSPFLTDGSFSTDAAVARGYTPEVVGAR
                     VVGAGGEHDRYYYTRKTELEGLPEVEICGNIADADALLVLSHGKGHGHTGFGGAIKNI
                     GMGCVSYRTRGAIHGLMSGHFAWEEERCTHCEQCVHGCPTGAVRFNEQGQLKISEHHC
                     RYCMHCTRSCPTEALSMEQPGEKFRLFQAGMAAAVKEALAFFDPANVYYITLLMNITP
                     LCDCWGFSTPPLVPDIGILASDDIVAIEQASLDLIDAEKLIPGTLPDQLCPPGEEGHL
                     FQRIHGKDPYEQVCQCAAAGLGNSDYALISVE"
     gene            complement(4836..5891)
                     /locus_tag="PLY56_00740"
     CDS             complement(4836..5891)
                     /locus_tag="PLY56_00740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20035.1"
                     /translation="MRTLVAIAILLAGATALHAAEAPPVLVYPCERVAAPPVVDGRLD
                     DAAWEKAALVSGFTYHDRRELVPVQTAFRVVYDAQALYVGVRCEEPLWQKMVPGAPGM
                     RDTHGAVFGGEAIELFIDPKHNHSDYYQIAANSDATLFDGKGTDSAWNSRAKVATRIS
                     EGGWSLEIALPWTDFEVRPEPGLVIGFNVCRDRNIAPDKLWSTWAPIAANFHDPLRFG
                     HLVLSATPEMIGRLGDEFRKGGLAGPIRVFAAEGFSGTTYLALAARSLETLEATLKEL
                     DSLRQQEKSPAVAAALAKRIAAAREMTAPIAVKVKGNQAFDAAEWVRADSQMQQLRLE
                     LEKAVWNARLEALLSGI"
     gene            complement(5985..6323)
                     /locus_tag="PLY56_00745"
     CDS             complement(5985..6323)
                     /locus_tag="PLY56_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009053036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOJ20036.1"
                     /translation="MKKVECIIRPQRLEEVKAALTELGVVGMTVLDVRGCGKQKGVTA
                     MYRGMEYTMTLLPKVKIEMVLHDDEVEDVVETLLQVARTGELGDGKIFISTLDDAIRI
                     RTGERGDAAI"
     gene            6557..7312
                     /locus_tag="PLY56_00750"
     CDS             6557..7312
                     /locus_tag="PLY56_00750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOJ20037.1"
                     /translation="MEKEFIAHLAIQAGEILKSRAGTAAVRAKNGTDIVTDADEASER
                     FIREEILRRYPDHRVLGEEYGSNEAEAEWEWVIDPLDGTVNFARGNSYFSVSIALAHQ
                     GKAQIGAVYRPFTGELFLAERGGGATLNGHPIHVSPIPRVEESIVILDWSRQETRQES
                     LARMERILLASHKIRTHGSAALDLCSVAAGQLEAFVHPRLAPWDFAAGQLIAEEAGGR
                     VTTLEGAERTLAAGSVLATNGKIHAEALALLQD"
     gene            complement(7360..9135)
                     /locus_tag="PLY56_00755"
     CDS             complement(7360..9135)
                     /locus_tag="PLY56_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ20038.1"
                     /translation="MPQQSLIGEFLRTADRHRNRVALAAKVDGEYRPSTYAETAERVR
                     RLGAHLIRMGVQPGDRVALFSENRPEWAISDLGTLAAGAVLVPLYATLPAPQVEYILR
                     DSGARVLIVTGQKRLEIALSVRANLPDLEQIIVIGAPAPEGTHAFETLVEEDAPEHRA
                     ESERRIAALGEADLATIVYTSGTTGEPKGAMLTHGNLLSNLSAMRPILRLTPNDIFLS
                     FLPLSHIFERSCGSYLPLTTGAAIYYAESLFTVAKNLEEVRPTIMMSVPRLYENMRQR
                     ILEAASKAKPAQRALVHWALRVGHERTRCRLERRFAGPGLRLRHAVAERLVLRKLRQR
                     MGGRIRYFISGGAPLSREACEFFFDLGVVLLQGYGLTETSPVVAVNRPEDPRPYGIGL
                     ALENLEVKIADDGELLVRGPNVMQGYWRKPEETQAAIDAEGWFHTGDIARMDPDGHLH
                     ITDRKKNLIVLSTGKKVAPQPIEGRLSTSPFIGDVLLLGDRHSTIGALVVPCFDALRE
                     QDGLAGLENAELAASPVVRQLIRAEIDRLSDGLAEFERIKRFAVLDREFSLEAGEITP
                     TLKPRRDVIMKHFAAQVQSIFAREA"
     gene            complement(9334..10983)
                     /locus_tag="PLY56_00760"
     CDS             complement(9334..10983)
                     /locus_tag="PLY56_00760"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20039.1"
                     /translation="MGRRCLFGTWVVLLWAGSLAWGADRPALRELSKLPTLTFDVGVS
                     FSFSRGRGLRLWWDFPDYPAEIAALEKSLKGDAWDAERVHRIGELAREMGDDARASKA
                     FERAVALYRERLKAQPGSGLLMASLGQALGDLGKIEEAEKTLREAARVAPKEWRCWML
                     LAQLLENQALAILTGPAAGGSAADEMLARAFAGVSPAHLPQPAELERAERLLDEARDT
                     HDRAVALAMETTQGNGPGAAKAHAERGGSRGFTQALLRGVLAQMRGQKVDPRQKMLSP
                     DSLADFRRAADLDPGSVELVALAGLCEVFGFALAQGHPWEAIWPALPEESKARVRRAM
                     ERLERLGQVKDARVAAAALDALGMVAYVASEEKRAEESLRRAVKRDPSRARTWEMLAM
                     ILGAGGRYEELVTWCKERLAYRDTVRTRLLLAKAYEKLGQLALAEEQVRAATALAPND
                     FYVSLGYAALLMRRSEAPKVLAEANGHLIRAEQACAAGCTLQQWVELVLTRAIHLGLS
                     GEEEKARGLLTECLNRDPGNKEVRQALAALGDGQKRPEERP"
     gene            11316..>12118
                     /locus_tag="PLY56_00765"
     CDS             11316..>12118
                     /locus_tag="PLY56_00765"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOJ20040.1"
                     /translation="MEIPLRYGLNPHQRQARLVFDGEGTPLRVLNGAPSYINILDALG
                     AWQLARELKQVAGVPGAASFKHVSPAGAAIARPLSPEFRASQMLPDEELSPVAQAYAR
                     ARGGDRMCSFGDFAAVSDVVDVSLASLLRREVSDGIIAPGYEPEALEILKQKRGGNYP
                     ILEIDPDYEPPAVEHRQVFGFELYQERNAASINPAALGTVVTEKKELPADVALTLAVA
                     TIALKYTQSNSVCVAYDGQVIGMGAGQQSRVHCTRLACGKADKWFCQQHP"
BASE COUNT         2053 a         4162 c         3909 g         1994 t
ORIGIN      
        1 cgcgcgcggg ctggcgctcc tatccgattg gcgcgggcgg gagcatctcg cgcagcgctt
       61 cctcttttcg gagcgcgaag gcggcactct gcgcccagat ctgctcggaa gcgagcgaga
      121 agagcgcctc gagctcggca acctgcgcca cgcacccctg gaactcggag tcatcacgcc
      181 ggggcgcctc gcgaatgcgc gagtacacct ccagcgcgtc tcggatccgg cagagaaggt
      241 cgaggggcag gatcgcatcc agggaatcgt agagggcatc gcagatgtag gcgtggcatg
      301 gatccgggcg gccaaatggg aggaggcagc ccgccggccc gagtgccgcg cacccgcgcc
      361 tattgtgagg gccagaaaga gcgtagaggc tatggcccaa gctctcgtcc gccagctcgc
      421 caagaagcgg gtcggtcagt atctccggca cgaagttggt gcgcctgcag caacagatgg
      481 agggatccgc ggcggtctcc gcggcctcgg cgcagcgcgc gcagaggtca ccaaagtgct
      541 ccgagaaaaa cgtcttcgat gcgctctccg ctcgcgcgta cagctcctgc caccgccgaa
      601 gtaccatcat ccactcccgg gcgcgcctgc cgatgccttc tccacctccg cgagcacggc
      661 gtcgagcgcc tcgcgcgtcc gtgcgccctc gatccgcccg agtaggcacc gatacgcctc
      721 ctccgcaatc gcgcccgcgt caaaccgggt gcgcagcacc cgggcaatca cgctccggcg
      781 agtcgccagc tccagctcct cctcgcgcag ctgcatgcgc tcctcccatc ctcgccgaag
      841 ccgtcacctc acctgcgctt tgggggcacg cgcagataga ccgcatcggc atccagaagc
      901 atgccctccc cgtcgaatgc ctccaccatc acctcatgcg tggcggcatc gtactgcgcc
      961 gggttccaca catactcgta cggcggcttc gagagacgtg ccacttcaaa gccgttgatg
     1021 agcacgcgca ccagcgccac ctggcccgcc gtctggatcc gaatcggcgt cggcttctgg
     1081 atcagggtgt ttgccggtgg cgaggtgatg cggatcccca ccgtgacggg catgctctcg
     1141 gtcaccagac tcgctttgcc ggcaccgttg accgccccga tggtgaaggt gaccgtctga
     1201 ccggcgacca gtgttggaag tgggtactca tacacctgag tgctgccgat ctgcttcatc
     1261 tcgccccgcc cgaggtcgcg ctcccccgcg gcaaccttca ctgtgacctc tttcacgccg
     1321 gtcgggtcga acaccaacgc gcgtagtgcc atcggctttc ccgcggcggg cggcgtgcgt
     1381 ggcgctgtca cgatagccgt gatgatcggg ccttcgacat ccttcggccg tgggaacggc
     1441 accaggggcg ccgagtcgtt ggcatcgaag aggccgtcgc catctgtgtc cttgttcttg
     1501 ggatcgaggc cgcacagctc ctcatggtag tcgctgaggc cgtcctcatc gctatccgtc
     1561 gctttgatat cgagcgcgca ctctcccgac cagctggtgc ggaagaggcc atcggtgatg
     1621 cgcaccttga aggtgagctt cccaccgcgc agggtaggcg ggatcgtcgc cacgtagtcg
     1681 tcgcccacct tcgccagcgg ctcggtgaaa tcgggcgagc cgttgatgtt gtacttcacg
     1741 cgggcatcga ttacgccgcc gctaatcgtc gccttgacgg tgatcggatc gttcggcccc
     1801 ggctcgcgtg gggaaaaagt gaccttcgtc agcttcgcca gcgccatcgt tggcccaacc
     1861 gtgtccagga agacgcgctc ggccaggaga tccaggaagt aatgcggggt cacgacccgg
     1921 atgccgcgcc ccaggcgcgc cgcgatttgg accacatcgg tgggcgtgac cttctcggca
     1981 tcgacgacgg ccaggacaaa ccgctgcttc tgggctgccg cgagaatcgc ctccgtcgct
     2041 ttctcgacgt cggtgacgtg caccgtctca ccgatcaggg gcttgccaag agagagcgtc
     2101 ggccccagcg aggtgccagc tgccaggaag ccgcgcaccg ccggctggag agccaggcgt
     2161 gcccacggcc tgtcgtggtc ggacgcggag acatcgatca cggagaccgc ggtgaggtcc
     2221 gcgcggctcg ccgcctcgtt cgtctcggcc acgaacgcct caaacgcctt cggatcagcc
     2281 ggaggcaggt agctggcgcc gctgggagcc atcacgaact ggtccacgcc ggcccaccag
     2341 gcatccacat agtaatagga gaggagcgcc ggggccaact cggccaggag cgggtgcagc
     2401 tcccagccga gagggatgta gccacgcgcg gcatcatccc acagagtgct catccgcccc
     2461 aggccaaaat cgaggttgcc gttgcccgag aagaggaagg tgacgtagct ggcgaggtgg
     2521 tgctccacca gccgcttgta ctggcgcagc ggggcgtagg cgggaacgcg cgcgtggaac
     2581 gagaggttgg ccacatccgc gccaggcacg aggcgcatcg ccgtggcgcg cgccagagcc
     2641 ggcatcgcgt cacctgggtc gcgggcgaat agcgcgcgcg tctccaccgg cgaaccgatc
     2701 cgctctcccc aaaagaggag cgcccctggt tccagcttcc ccaagagcgt gcgcaggagc
     2761 tccgcctctt ccttcacccc agggttcagg cccacgggca gaatcttctc ggcgaccagg
     2821 tagtcgcgca ggcccacctg ctctggcttg agcacggcga gcttcgcccg gttgcaccgg
     2881 gggagcaggc tctcaatggc ccagtgacac gcctcgcggc gatccttgaa acgcgtcagg
     2941 tcttccacca gcttgcggct cgcatcacgc gccgcggccg tgacggggag gacgtcctcc
     3001 aggccggcga gagccgtcgc caggttgatc gtctccggct gggccggatc gtagaggacc
     3061 tcacccttga tccgatccgc gaagcgggca atcgcctccg cgggcgtctt ctcctcgggc
     3121 ttgaagccgc gggcggcgta gacctggtcg aaccaggcgc ggtcctgcgc ggtgtgaacg
     3181 cagtacagca ccggtcgctc ggcgcctcgg ttgacgatcc cctgaaggca agcgagcgcg
     3241 agcttcgtgg aggcatcggc ttctcgcaag tctaccaggg taaccggcac cggcacagtc
     3301 tcggcggcgt gcgctgggcc gagaccggcc aggagaacga gcaagatcat cggtagaatg
     3361 ctcaggattc tcatgagttc cgcccagatg gcaagagcgc tccgggcttc ccgccgcgag
     3421 cgcgactgcc cgtggcgtgc cgggatcttg cccggctcca ttatactcgg gggtgggatg
     3481 ccggtcaagg cgcactccgt gggcgcccgc gccacatggc gcgcgggcgg gattcgggtg
     3541 catggcccgc gaatcaaatt cctgatatac gccctgcggg cctcgcccga ggcggccgtg
     3601 agttgtcctg gaggttggag gagcgatgac gagtcaggtt ctttttgcct cggcgcatgt
     3661 gacgcgcctg gatcgagatg cgacgcttcc cgcgaagttc gagcggttgc tggagaaagc
     3721 agatctggcg ccacgctttg gtggcaagcg cgtggcgatc aagatgcacg ttgggagtgg
     3781 gatcgggttc tacaccatcc acccgctctt cgtgcgccag gtggtcgacg cggtgaaaaa
     3841 agcgggaggg tctcccttcc tgacggatgg ctctttctca accgatgccg ccgtggcccg
     3901 gggctacacc cccgaggtag tcggcgcgcg cgtcgtgggc gccggcggtg agcatgaccg
     3961 ctactactac acgcgcaaga cggagctgga ggggctgccc gaggtcgaga tctgtggcaa
     4021 tatcgccgac gcggacgccc tgctcgtcct gagccacggc aaggggcatg ggcacaccgg
     4081 cttcgggggc gccatcaaga atatcggaat ggggtgcgtc agctaccgga cgcgcggtgc
     4141 catccacggg ctcatgtctg gccacttcgc ctgggaggag gagcggtgta cccattgtga
     4201 gcagtgcgtc catggctgtc ccacgggggc ggttcgcttc aatgagcaag gccagctgaa
     4261 gatctcggag catcactgcc gctattgcat gcactgcacg cgctcctgcc ccacggaagc
     4321 cctgagcatg gagcagcccg gggagaagtt ccgcctcttc caggccggca tggccgctgc
     4381 agtcaaggag gccctcgcct tcttcgatcc ggcgaatgtc tactacatca ccctgctgat
     4441 gaacatcacg ccgctttgcg attgctgggg cttctccaca cccccgctcg tgccggatat
     4501 cgggatcctc gcgagcgacg acattgtggc gattgagcag gcgtcgctgg acctcatcga
     4561 tgcggagaag ctgatcccgg gcacgctgcc ggaccagctc tgcccgcccg gcgaggaggg
     4621 ccacctcttc cagcgcatcc acggaaagga cccctacgag caggtgtgcc agtgcgccgc
     4681 cgcgggtctg ggcaacagcg actacgcgct catctcggtg gagtgaggta tccgatccgg
     4741 ccacacctgc gcgaggctgc ccgggtgtct gcccggcggc ctcgctccac tcgctcgggg
     4801 tatctggatc tattgtggat caagcggggc gcgcgtcaga tcccgctcag gagggcctcc
     4861 aggcgcgcgt tccacaccgc cttctccagc tcgagacgca gctgctgcat ctgcgagtcg
     4921 gcgcgaaccc attcggcggc atcgaacgcc tgattcccct tcaccttgac ggcaataggc
     4981 gccgtcatct cgcgtgcggc ggcaatccgc ttcgcgagcg cggcggcaac agccggcgac
     5041 ttctcctgct ggcgcagcga atcgagctcc ttcagcgtgg cctcgagcgt ttccaacgag
     5101 cgcgcggcca gcgccaggta agtggttcca gagaacccct cggcggcaaa cacccggatc
     5161 ggacccgcga ggcctccctt tcggaactca tcgcccaggc ggccgatcat ctctggggta
     5221 gccgagagca cgaggtgccc gaaacgaagt gggtcgtgga agttggccgc gattggcgcc
     5281 caggtactcc aaagcttatc gggagcgatg tttcggtcgc gacacacatt gaagccgatg
     5341 acgagaccgg gctccgggcg cacctcgaag tccgtccaag ggagcgcgat ctccaggctc
     5401 cacccgcctt ccgagatgcg cgtcgccacc ttcgcgcggc tattccaggc agaatcggtg
     5461 cccttgccat cgaagagggt ggcgtcgctg ttggccgcaa tctggtagta atctgagtgg
     5521 ttatgcttcg gatcgatgaa gagctcaatc gcctcgccgc caaagacggc gccgtgggtg
     5581 tcgcgcattc caggcgcgcc cgggaccatc ttctgccaga gcggctcctc gcagcgaacg
     5641 cccacgtaaa gcgcctgggc atcgtagacg acgcggaagg cggtttgcac cggcaccagc
     5701 tcgcgccggt cgtgataggt aaacccgctg acgagcgcgg ccttttccca cgccgcgtca
     5761 tcgagccgac cgtctactac gggaggtgcg gccacacgct cgcagggata gacgagaaca
     5821 ggaggcgcct cggccgcatg gagagcggta gcgccggcca gaagtatggc aatggcgacc
     5881 agggttcgca tcggtgtcac cttcccttcg cgcgcgccgg cggcgccatg atcgcgacac
     5941 cctgaccggc acccggagcc ggggccaggc tccacgggat tggatcagat ggcggcgtcg
     6001 ccgcgctcgc ccgtacgaat gcgaatggcg tcatcgagag tgctgatgaa gatcttgccg
     6061 tcgcccaact caccggtgcg cgccacctgc agcaacgtct cgacgacgtc ctccacctcg
     6121 tcatcgtgaa ggaccatctc gatcttcacc ttgggcagca gggtcatcgt gtactccatc
     6181 ccgcggtaca tcgccgtgac gcccttctgc ttgccgcagc cgcgcacatc cagcaccgtc
     6241 atgccgacga cacccagctc ggtaagcgcc gccttcacct cttccagccg ctgaggacgg
     6301 atgatgcact cgactttttt catacccctc tctccctcgc ccattatagc acgcaggtag
     6361 gaggggcgca accacgtttg acacggcgca ctggcccgga tataatgcga acaggcggtt
     6421 gccacgggcg acgcgctcct cggtgaagtg cgcccacagt tccggcagac cgccccgaag
     6481 cacccgctct cgccgatctg gccccaccgc tcgaggaagc agcgcgcgag agctcatcag
     6541 aggaatcgag aagagcatgg agaaggagtt tatcgctcac ctggccatcc aggccgggga
     6601 gatcttgaag agccgcgcgg gcacggctgc cgtccgcgcg aagaatggca ccgatatcgt
     6661 gacggatgcc gatgaagcct cggagcgctt catccgcgaa gagatcctcc gccgctatcc
     6721 ggatcatcgg gtgcttggcg aggagtatgg aagcaacgag gcagaggccg aatgggagtg
     6781 ggtgatcgac cccctggatg gcacggtgaa cttcgcgcgc ggcaactcct acttctcggt
     6841 gagcatcgcg ctggcgcatc agggcaaggc gcagattggc gcggtctacc ggcctttcac
     6901 cggcgagctg ttcctcgccg aacggggcgg cggcgccacc ctcaacggcc acccgatcca
     6961 cgtctccccc atcccgcgcg tggaagagag catcgtcatc ctcgactgga gccgccagga
     7021 aacgcgccag gagagcctgg cgcgcatgga gcgaatcctg ctggcctccc acaagatccg
     7081 cacgcacggc tcggcagcgc tggatctctg tagcgttgcg gccggccagc tggaggcgtt
     7141 cgtccacccc cgcctggcgc cctgggactt cgccgccggg caactcatcg ccgaggaggc
     7201 cggcgggcgc gtgacgaccc tggaaggggc agagcgcacc ctcgccgccg gaagcgtgct
     7261 ggccaccaac gggaagatcc acgcggaggc tctcgccttg ctgcaagact gagcggcaac
     7321 gccgcggcgc cgcctggccg gaggccgtgc ggcgccggtt caggcctcgc gggcaaagat
     7381 gctctgcacc tgagccgcaa agtgcttcat gatcacatcc cggcgtggct tgagggtggg
     7441 cgtaatctca ccggcttcga gggagaactc tcggtcgagc acggcgaatc gcttgatccg
     7501 ctcgaactcg gcgaggccgt cgctcaggcg gtcgatctca gcgcggatca gctggcgcac
     7561 caccgggctc gcggcgagct ccgcattctc caaccccgcc aggccatctt gctcgcgcag
     7621 ggcatcgaaa cagggcacca ccagggcgcc gatggtgctg tgccgatccc caaggaggag
     7681 cacgtcccca atgaacgggc tggtgctcaa tcggccctca atgggctggg gcgcaacctt
     7741 cttgccggta gagaggacga tcaggttctt cttgcggtcg gtgatgtgga ggtggccatc
     7801 cggatccatc cgggcgatat cgcccgtgtg gaaccacccc tcggcatcga tagccgcctg
     7861 ggtctcttcc ggctttctcc agtatccctg catcacattg ggcccgcgca ccagcaactc
     7921 gccatcgtcc gcgatcttca cttcgaggtt ctcgagcgcc agaccgatcc cataggggcg
     7981 cgggtcctcg gggcggttca cggccaccac gggagaggtc tccgtcagcc cgtagccctg
     8041 aaggagcacc acccccaggt caaagaagaa ctcgcacgcc tcgcgggaga ggggagcgcc
     8101 tccggagatg aagtagcgaa tccggcctcc catgcgctgg cgcagcttgc gcagcacgag
     8161 gcgctccgcg accgcatgcc gcaggcgcag gcccggcccg gcgaaacgcc gctccaaccg
     8221 gcagcgcgtg cgttcgtgtc cgacgcgcaa cgcccagtgc accagcgcgc gctgggccgg
     8281 cttcgctttc gacgccgcct cgaggatgcg ttgccgcatg ttctcataga gacggggcac
     8341 gctcatcatg atcgtggggc gcacctcttc caggttcttg gcgaccgtga agaggctctc
     8401 cgcatagtag atcgcggcgc cggtggtcag aggcagatag ctgccgcaag agcgctcgaa
     8461 gatgtgcgag agcggaagga acgataggaa aatgtcattc ggagtgaggc ggaggatcgg
     8521 gcgcatggcg ctgaggttcg aaaggaggtt gccgtgggtg agcatcgccc ctttcggctc
     8581 gcccgtcgtt ccggaggtgt agacaatcgt cgccagatct gcctcaccca gggccgcgat
     8641 gcggcgctcg ctctcggcgc ggtgctcagg ggcatcctcc tccactagcg tctcgaacgc
     8701 atgcgttccc tcaggagctg gtgcgccaat gacgatgatc tgctccaggt cgggaaggtt
     8761 ggcgcgcacg gaaagagcga tctccaaccg cttctgcccc gtgacgatga ggacgcgcgc
     8821 tccggagtcg cgcagaatgt actccacctg gggcgccggc aacgtcgcgt agaggggcac
     8881 gagcacggcg ccggctgcga gggtgcccag gtcagagatc gcccattcag ggcggttctc
     8941 gctgaagagt gccacccggt caccaggctg cactcccatc ctaatgaggt gggcgcctag
     9001 gcgtcggacg cgctctgcgg tctcggcgta ggtactgggg cggtactctc catcgacctt
     9061 ggcagccagg gccacgcggt tgcgatggcg atccgccgtc cggaggaatt ccccgatcag
     9121 gctctgttgc ggcatcgttg tgatcctcta tagttcagat gattcgatag cgaaggcata
     9181 ggggatgcaa gaggcactag ggcgcatggt gtgatactct cctcgcggta gattcgcaat
     9241 cgatggggcc agatcctgcc aggaatctat ttgtcgcacg agacgcacag ccagggcccc
     9301 atctcaccac ccgggaacag ttcctatccc gcgtcagggg cgttcttcgg ggcgtttctg
     9361 gccatccccg agcgcagcga gcgcctggcg cacttccttg ttgcctggat cgcggttgag
     9421 gcactctgtc agcaagccgc gcgccttctc ttcctcgccg gagaggccaa gatggatcgc
     9481 gcgggtgagc actagctcga cccactgctg cagcgtgcat cccgcggcgc acgcctgttc
     9541 cgcgcgaatg aggtgtccat tcgcttcagc caggaccttg ggagcctcgc tccggcgcat
     9601 gaggagggcg gcatatccga gcgagacgta gaaatcattc ggggcgagcg ccgtagccgc
     9661 gcgcacctgc tcctcggcca gcgccagctg gcccagcttc tcatatgcct tggcgaggag
     9721 gagccgggtg cgcaccgtat cgcggtatgc cagacgctct ttgcaccacg tcaccagctc
     9781 ttcataccgc ccaccggcgc ccaggatcat cgcgagcatc tcccaggtgc gcgcgcgcga
     9841 agggtctcgc ttcacggcgc gccgcaggct ctcctcggcg cgcttctcct cgctcgcgac
     9901 gtaagcgacc attcccaggg cgtccaacgc cgccgctgct acgcgcgcgt ccttcacctg
     9961 ccccaggcgt tcaagacgct ccatcgcccg gcgcacccgt gccttcgact cctcggggag
    10021 ggccggccag atcgcctccc agggatgccc ctgggccagc gcaaacccga agacttcgca
    10081 cagccccgcc agcgccacca gctccacgct gcccgggtcg agatcggcag cacgccggaa
    10141 gtcggcaagt gaatcgggag agagcatctt ctggcgggga tccaccttct ggcctcgcat
    10201 ctgcgccaga acgcctcgga gcagcgcctg ggtgaacccc cgagagccgc cgcgctccgc
    10261 gtgggccttg gcagcccccg gtccattccc ctgggtagtc tccatcgcga gggcgacagc
    10321 ccggtcatgg gtatcgcgcg cctcatcaag aagacgctcc gcgcgctcca gctcggccgg
    10381 ctgggggaga tgagcaggcg aaacgccggc aaacgcgcgc gccagcatct catccgccgc
    10441 gcttcctccc gcagccggac ccgtcaggat agcgagtgcc tggttctcca gaagctgcgc
    10501 caggagcatc cagcagcgcc actcctttgg ggcaacgcgc gccgcctccc gcagcgtctt
    10561 ctctgcttcc tcgatctttc ccagatcgcc cagcgcctgg ccaagcgagg ccatcagcag
    10621 accgctcccc ggttgcgcct tcagccgctc gcgatagagg gccacggcgc gctcgaacgc
    10681 cttgctggcg cgcgcgtcgt cgcccatctc gcgcgcgagt tcgccgatgc ggtgcacgcg
    10741 ctcggcatcc caggcatcgc ccttcagcga tttctccagc gcagcaatct ccgccgggta
    10801 atcggggaag tcccaccaca gccgcagccc gcgcccccgg gagaacgaga aggagactcc
    10861 tacgtcaaag gtgagcgtcg gcagcttcga cagctcgcgc aacgcgggcc ggtccgcccc
    10921 ccaggcgagc gagccagccc acaggaggac cacccaggtc ccgaacagac agcgtcttcc
    10981 cacttgatcc cctctcccag cggcccggcc gcctcccatc agcattcgcg tgccaccctt
    11041 gaggcccggc aactcacctc gccgcggccc atttcccggg cgatggcgcg cacctcgctg
    11101 cgatgtaggc catgcgccaa aacagattcc ctcttctctc atccacctgg tgaattcctc
    11161 tcgcgcctgc aagccgcgcc cccgcgacac gcatccccag gccccttccc aggccaccga
    11221 ccgcgtccgg aacgaggcgc aggggcggta ggaagcggcg ctcgtggcgg cgaaaccagg
    11281 agtatcgata caccacgcgc ttgaggagag agactttgga gatcccactt cgctacggct
    11341 tgaaccccca ccagcgccag gcgcggctcg tcttcgatgg agaaggcacg ccgctgcgcg
    11401 tgctcaacgg cgcgccgagc tatatcaaca tcctcgatgc cctcggggca tggcaactcg
    11461 cccgggagct aaagcaggtc gccggcgttc caggcgccgc gtccttcaag catgtcagcc
    11521 ctgccggcgc cgccatcgcc cggccgctct cgccggagtt tcgcgcttcc cagatgctcc
    11581 cggatgagga gctctcgccc gtggcacagg cctacgcgcg cgcccgcggc ggcgaccgca
    11641 tgtgctcttt cggcgatttt gccgcggtga gcgacgtggt tgacgtctcc ctggcctcgc
    11701 tcctgcgccg cgaggtctcc gatggcatca tcgctcccgg ctacgaaccg gaggccctcg
    11761 agatcctcaa gcagaagcgt ggaggcaact atccgatcct ggagatcgat cccgattacg
    11821 agccgcctgc cgttgagcac cgccaggtgt ttggctttga gctgtatcag gagcggaacg
    11881 ccgcctccat caatccggcg gcgctcggca cggtcgtcac cgagaagaag gagctgcctg
    11941 ccgatgtggc gctcaccctt gccgtggcca ccatcgcgct caaatacacc caatccaact
    12001 cggtatgcgt ggcgtatgac ggccaggtga tcggcatggg cgccgggcag caatcgcgcg
    12061 tccactgcac ccgcctggcg tgcgggaagg cggacaagtg gttctgccag cagcaccc
//
LOCUS       DAOSCV010000014        11848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3635_length_11848_cov_3.000254, whole genome shotgun sequence.
ACCESSION   DAOSCV010000014 DAOSCV010000000
VERSION     DAOSCV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3635_length_11848_cov_3.000254"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PLY56_00770"
     CDS             complement(<1..813)
                     /locus_tag="PLY56_00770"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOJ20041.1"
                     /translation="MNAIEVHATSAMVIGPGQAVAAGIETLQSGGNAVDAAVATALAA
                     GVVAPAQCGVAGFGGALITYLAAQKRVACLEFGAMSPAGVTPGWLLAAGEDAFPMGAR
                     AVMVPGTAAGLTRAVAAYGSRPLAQLVAPAVRLAREGFPASPGYVADLLAHRERIERF
                     PHTAEWLLPDGQAPRLGSLITNEALARLLERLAAEGLDSLYRGEAAADLVAHVQASGG
                     VLTLDDLADYTPRETEPLSLSFGAYTIHTSPLPTGGAVLAQAIKTLDGLDLAA"
     gene            1059..1817
                     /gene="rsmG"
                     /locus_tag="PLY56_00775"
     CDS             1059..1817
                     /gene="rsmG"
                     /locus_tag="PLY56_00775"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOJ20042.1"
                     /translation="MAEREEQGAVVLPDRPAEELIREGAESLGIPVGPGELGQYAAYV
                     RELLDWNQRMNLTAVTDAREIAVKHILDSLTCFLAVSAGKGVRVLDIGTGAGFPGMVL
                     KIHAPETPLTLLDSTRKKLDFLEHLAKALGLSGVETLHARAEDAGRDRRYRERFDLVT
                     ARAVAELRVLLELCLPFVRVGGLFLAMKGPRGDEEVGPAKRALSLLGGEIERVIPFSL
                     PFGAGERRLILVRKRRPTPAAYPRKAGTPSRAPL"
     gene            complement(1831..3120)
                     /locus_tag="PLY56_00780"
     CDS             complement(1831..3120)
                     /locus_tag="PLY56_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ20043.1"
                     /translation="MHDADESLATSVVEAPAELDEQNKRDYRFNMVACIIDDGGYAGG
                     ISLLSMTTILPLFVRQLTDSAFLVGLIPAIYWAGIFLPQLFVARLVSHFPRLRPWVMS
                     IAFFERIFLVLLAPLTLWLWQKPGALLVAFFLMWTGYALSTGLNFATYLGLVAKIIPA
                     NRRGLLYGLSGALGGVLGVAGAKLAEYLLQDGGMPYGFAWCFGIGSAVLAVTVVPLGF
                     VREPVGKPPEERPHAGPRQIVRLLRGDPAFARFVWGCIAYAFAGAAPAFYTVHAIDHL
                     GATAADVARFTAVLTATTVVTAPAWGWLADRTGNRRVVLGSAILAGMGLLVALLAPSL
                     GWFYLVFALNAAASPGMGFAVANIILEYGPGAKAPAYVAVHSSCTMPASALAPLLAGG
                     LAQTAGYHAVFVAGAILAGLAILGYWRAPEPRERALG"
     gene            complement(3305..4186)
                     /locus_tag="PLY56_00785"
     CDS             complement(3305..4186)
                     /locus_tag="PLY56_00785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ20044.1"
                     /translation="MEISIRDATLVAAGFRTVGEGLDFLGLSSVELAVDRDHQVSAIR
                     PGENYRRMPIETDDDVEELRRHLEDTGVTVSAFLLSNDFGRADLDREIAWVTRTVEAA
                     GALGIPVVRIDTVMHGEHDRPVDERQRRFVEVVTRILNATAEMEVCLGIENHGAQGND
                     PAFHSGLFAEVGHPRFGLTLDTGNFYWSGMPLTRVYEVIAQFAPYARHTHVKNIGYPP
                     SERERERELGWRYADFVCPLEEGDIDLERVHSILRDAGYDAALCIEDESLGRFAPTER
                     REVLRRDVAYLQRILGA"
     gene            complement(4346..5305)
                     /locus_tag="PLY56_00790"
     CDS             complement(4346..5305)
                     /locus_tag="PLY56_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ20045.1"
                     /translation="MPLVDLPLAQLHEYKGRNPRPADMDEYWDRALAEMRALDPRVEL
                     EPYPLKTHHAECFHLWFTGVGGARVHAKYLRPKNAPEPHPAVLLFHGYSGNSGDWADK
                     LAYVSQGYSVAALDVRGQGGLSEDPGGVKGNTLRGHIIRGLDDAPEKLFFRQVYLDCA
                     QLARIVMSMPEVDAKRVGATGGSQGGGLTLACAALEPGIKRAAPVFPFLCDYKRVWEM
                     DLAVAAYEELKTYFRLFDPLHEREEAVFTRLGYIDNQHLAHRIRAEVMMFVGLMDTVC
                     PPSTQFAAYNKIEAPKSVVIYPDYGHEGLPGANDRVFDFMAGL"
     gene            complement(5433..9086)
                     /locus_tag="PLY56_00795"
     CDS             complement(5433..9086)
                     /locus_tag="PLY56_00795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ20046.1"
                     /translation="MSGADFVHLHNHTEFSLLDGACRVKELVERVAELGMPAVALTDH
                     GVMYGAVEFYDACKKAGVKPILGCEVYMAPRGRTDRDPVLDREQYHLVLLAENLTGYK
                     NLMKLVSIASLEGYYYKPRVDREILAEYSEGLIAASACLGGELPSHILAGNMQKAREL
                     AGWMAEVFPGRYYLELQDHGIPEQRTVNEAILQLARELNLPLIVTNDLHYLRKEDAQA
                     HDVLLCIQTNTTVDDEKRMRFHAPEFYLKDVEEMARLFPDHPEAMRNTVEIAERCNVE
                     LTFQQVLPHYEVPPGYTYESYLEKLCRDNIPRRYREFTPEMEARLRFELDVIQSKGFS
                     AYFLIVNDFVHFARSRGILAQARGSAAGCMVTYLLGISSIDPIENDLMFERFLRIDGK
                     KMPDIDVDFEDLRRDEVLQYVVEKYGADCVAQIITFGTLGPKAAVRDAARALQVPRSD
                     VDRICKLIPTMPSSPPLRHFLETIPELRSEYESNPEIRSLFDTAQGIEGLSRHASTHA
                     AGVVISKEPLTNIVPLQRTGDSPIPTTQFDFRIVEEVGLLKMDFLGLSYLTVVSKALH
                     LIEETTGKKLTLDQIPIDDERTYQMLGNGDAMGVFQVESSGMRQLLRDLKPRTFKDVA
                     PLIALYRPGPLQSGMVQDFIARRHGRAKVEYPHPMLEPVLKDTYGILLYQEQVMKIAM
                     VMGGFSAVQAEALMKAMSKKKQDVMDKNRPLFIEGAEARGVPKKTAEHIYELMANFAK
                     YGFNKSHSAAYALLMYQTAYLKCNYPVQYLAALMTAFMENKDKVVTYVDECRHFGIPV
                     LPPDINKSQADFSVEPFEPEEGASDGEGPRYAVRFGLAAIKNVGRDEIEQIVAERANG
                     PFRSLHEFCTRIGGSGALNRATIECLIRAGAFDSTGARRAQLIAALDQAIALGQRARK
                     EREVGQFSMLDLLAGDAGQPEESPEESLPDVPEFSREELLAAERDLLGFYLTDHPVME
                     YRPALQRMRVLTTQEVAEREDKEVVRVGGIISRIRRLNTRKGDPMMVIGLEDWLGTVE
                     VIIWPSVLQEFAPHLQLEKVVVIEGKVSSKDSGKEENGEDEAPSRGQVEIIAESVRPV
                     RKPAEGGEAGLHAPANGNGHANGYRNGTNGISPTHPGGEPDTIVPDEPETPQGDPVII
                     QVPRAHMRARFLHELRSAVQAYPGDRRLLLQLEVAPGDRYTIECQGFGIQPAPELVQR
                     VHELLGPRCMLGI"
     gene            9631..10029
                     /locus_tag="PLY56_00800"
     CDS             9631..10029
                     /locus_tag="PLY56_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ20047.1"
                     /translation="MDLGIDQGEFTNRPERCAEVGVEDLFAVRVSGNAVVECQGEMDI
                     CAEAPLREALARAMHDHPTQVQVDLSQVRYIDSACIAVLLRASDTLRQQGGSLVLTAA
                     SPVVCRALSLLGLDQRLRPGGIGEPPRGSS"
     gene            10322..11344
                     /locus_tag="PLY56_00805"
     CDS             10322..11344
                     /locus_tag="PLY56_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ20048.1"
                     /translation="MMQSVLTNLLEDEWQKADPETWAKRRAAIHAAFTELLGEGVVAP
                     PEDRDLQWHGEERIDGLRIRKLSFLAEPDDRIPAYLVMPEELSAPAPAVLCLHGTTVD
                     AKEACLGRGSHPGGSKGTAVDLARRGFITLAPDHFNAGERLKPGEKPYDSGPLYARHP
                     GWSDMGKAIYDHRLCVDLLQTLPEVDGDRIGCIGHSLGGYCTLFLAAMDERIRAGVSS
                     CGVTVWAADPNRLNWSRDQPGRYVHFPKLREYWNSGRQAPVEFHEIMALVAPRAFLNI
                     SAVGNDVCFPVFAPFAELYVQVESVYKLLGAEGKFAAHFHSCGHSFGPSARALAYAWL
                     AEQLEL"
     gene            complement(11435..>11848)
                     /locus_tag="PLY56_00810"
     CDS             complement(11435..>11848)
                     /locus_tag="PLY56_00810"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HOJ20049.1"
                     /translation="ADFSAVLLQEDLNAQLHERSSLIRGLKLSITPEGARLRGSADLP
                     GVKLPVTPDFLLEGTLKIDGEGRLILDASKIRVVGVEVPEFAAKLLASQVNPLVDLSS
                     ARLPVYLRTVEPDHGELRLTGRARVRTGSYADLDS"
BASE COUNT         1983 a         3856 c         3986 g         2023 t
ORIGIN      
        1 cgccgcgagg tcgagcccgt cgagcgtctt gatggcctgg gccaacaccg ctcccccggt
       61 gggcagcggc gaggtatgga tcgtgtaggc cccgaaggag agcgagagcg gctccgtctc
      121 tcggggcgtg tagtcggcca ggtcgtcgag ggtcaatacg ccaccgctcg cctgcacgtg
      181 cgcgacgagg tccgccgcgg cctctccccg gtagagcgag tccaagccct cggcggcgag
      241 gcgttccagg aggcgggcga gcgcctcatt ggtgatcagg cttcccagtc gcggtgcctg
      301 gccatccgga aggagccatt cggccgtgtg cgggaaccgt tcgatcctct cacggtgggc
      361 gaggaggtcg gccacatacc cgggagaggc cgggaagccc tcgcgcgcca ggcgcaccgc
      421 gggcgcgacc agctgcgcca gtggacgcga gccgtacgcg gcgactgcgc gggtcaagcc
      481 ggccgcggtt cctggcacca tcaccgcgcg cgcgcccatc ggaaaggcgt cctctccagc
      541 ggcgagcagc cagccgggcg tgacacccgc gggcgacatg gcgccaaact ccaggcaggc
      601 gacccgtttt tgagcggcga ggtaggtgat cagcgcgccg ccgaagccgg ccacgccgca
      661 ttgcgcgggc gcaaccaccc ccgccgccag cgccgtcgcc accgcggcat ccacggcgtt
      721 gccccccgat tgaagcgtct caatcccggc ggcgacggcc tggcctggcc cgatcaccat
      781 cgccgatgtg gcatgtacct cgatcgcgtt catgcctccc ctggagcata cttacccttt
      841 cgcctggacc aacccacatg caggcggcct cccgtggcgc ccggacgcaa tccaccgaga
      901 acacacttcc ggcgcccggc gcacgccagg aacggcggcg acggcgccga acctcccagt
      961 ggcgcgagat cgcatcgcgg gtgataccag cgcgcctggg taggttgcgc gtttcaaccc
     1021 gagggcactt cacaggcccg ggggaagagg gagcggcttt ggcagagaga gaagagcagg
     1081 gagcggtggt actgccggat cgtccggcgg aggagttgat ccgcgagggc gcggagagtt
     1141 tgggcatccc ggtggggccc ggagagttgg ggcagtacgc cgcgtatgtg cgcgagctcc
     1201 tcgattggaa ccagcgcatg aacctgacgg cggtgaccga cgcgcgcgag atcgctgtca
     1261 agcatatcct cgattccctc acctgcttcc tcgctgtctc ggctggcaag ggtgtgcgcg
     1321 tgctcgatat cggcacaggc gccggcttcc ccggaatggt gctgaagata cacgccccgg
     1381 agacgccgct caccctcctc gatagcacgc gcaagaagct cgacttcctg gagcacttgg
     1441 cgaaggcgct gggtctctcc ggcgtggaga cgctgcacgc ccgcgccgag gatgccgggc
     1501 gcgaccggcg ctaccgcgag cggtttgacc tggtgacggc gcgcgccgtg gctgagttgc
     1561 gcgtgctcct ggagctgtgc ctcccctttg tgcgcgtcgg cggcctcttc ctcgccatga
     1621 aaggaccccg cggagatgag gaagtggggc ccgcgaagcg cgctctctcc ttgctgggcg
     1681 gggagatcga gcgcgtgatt cccttctcgc tccccttcgg ggccggcgag cgtcgcctca
     1741 tcctggtgcg caagcgccgg ccaacgcccg cagcctatcc gcgcaaggcc ggcacgccct
     1801 cgcgcgcgcc actctagcgt gcgcctgggg ttatcccagc gcgcgctctc gtggctcggg
     1861 ggcgcgccaa tagccgagga tggccagtcc tgccaggatg gcgccggcaa cgaagaccgc
     1921 gtggtagcca gccgtctgcg ccaggccgcc tgcgaggagc ggggcaagcg cgcttgccgg
     1981 catcgtgcag gacgaatgga cagccacgta ggcgggtgct ttcgcgcccg ggccatactc
     2041 caggatgata ttcgctacgg caaatcccat cccaggcgag gcggcggcgt ttagcgcgaa
     2101 aaccaggtag aaccacccta gcgagggcgc gagcagcgcg acgagcaggc ccatcccggc
     2161 gaggatcgcg gagcccagaa cgacccggcg gttgcccgtg cggtcggcga gccagcccca
     2221 ggcaggcgcg gtcaccaccg tagtggcggt gagcaccgcc gtaaaccggg ccacatcggc
     2281 ggcggtggct cccaggtggt cgatggcgtg gacggtgtag aaggcaggcg cggccccggc
     2341 gaaggcgtag gcgatgcacc cccacacaaa gcgcgcgaag gcgggatcgc cgcgcagcag
     2401 gcggacgatc tggcgtggac cggcgtgtgg gcgctcctcg ggcggtttcc cgactggctc
     2461 gcggacaaag ccaagcggca ccacggtcac ggcgaggacc gccgacccga tgccgaagca
     2521 ccaggcgaag ccgtagggca tcccgccgtc ctggagcaga tactcggcca gcttcgcgcc
     2581 cgccaccccc agcacgccgc ccagggcgcc gctgaggcca tagaggagcc cgcgccgatt
     2641 cgccgggatg atcttggcga cgagcccgag gtaggtggca aagttgagcc ccgtgctcag
     2701 ggcgtacccc gtccacatca ggaaaaacgc caccagaagc gcgccgggct tctgccacag
     2761 ccagagcgtg agcggtgcca gcagcaccag aaagatgcgc tcgaagaagg cgatagacat
     2821 cacccagggc cggaggcgcg ggaagtggga gacgagacgc gccacgaaga gctgcggcag
     2881 gaagattccc gcccaataga tcgccggaat caaccccacc aggaaggccg aatcggtgag
     2941 ctggcggaca aacagcggga ggatggtcgt catggagagg agactgatcc ctcctgcata
     3001 accaccatcg tcgatgatgc acgcgaccat gttgaagcgg tagtcgcgct tgttctgttc
     3061 gtctagctct gccggggctt ctaccaccga tgttgcgagt gactcgtctg cgtcatgcac
     3121 aagataggat tcgccgcggt cgtgcctctc cctgccccga gtggagtgaa cggcttcttc
     3181 ccctggcgcg ccagggcgct atgggatcac cgtgcgcacg cgaatagcct tgggggccgc
     3241 cacaatgccg tttcgtgccg cttgcgttcc gctgcggccg ctcccggata cctcggaggt
     3301 ggcgctatgc tccgaggatg cgctggagat acgcgacatc gcggcggagc acttcgcgcc
     3361 gctccgtggg ggcgaagcgc ccgagcgact cgtcctcgat gcagagcgcg gcatcgtaac
     3421 ccgcatccct gaggatgctg tgtacccgct cgaggtcgat atcgccctcc tccagggggc
     3481 agacgaaatc ggcataacgc cagccgagct cgcgttcgcg ctcacgttcc gagggcggat
     3541 agccgatatt cttgacgtga gtgtgccggg catacggcgc gaactgcgcg atcacctcat
     3601 acactcgggt gagcggcatt ccggaccagt agaaattgcc cgtatccagg gtcaacccaa
     3661 agcgcgggtg gcccacctcg gcgaacaggc cggaatggaa ggccgggtcg ttcccctgag
     3721 cgccgtggtt ttcgatgccc aggcagactt ccatctccgc ggtcgcgttg aggatgcgcg
     3781 tcaccacctc aacgaaacgc cgctggcgct catccaccgg acggtcgtgc tcgccatgca
     3841 tcaccgtgtc gatgcgcacc acagggatgc ccaaggcgcc cgctgcctcc acggtgcgcg
     3901 tcacccaggc gatctcgcgg tctagatcgg cccggccaaa gtcgttcgat aggaggaagg
     3961 cggagaccgt cacacccgta tcttccagat gccggcgcag ttcctccacg tcgtcatcgg
     4021 tctcgatcgg catgcggcgg tagttctctc ccgggcggat ggcagaaacc tggtgatccc
     4081 ggtcgacagc aagctccacg gacgaaaggc ccaggaaatc gagaccctca ccgaccgtgc
     4141 ggaaaccagc cgccaccagc gtggcgtcgc gaatggaaat ctccatggcg tgcctccctg
     4201 ttcacactcc cacgccgtca gcatgtggat ccttggcaga gcccgagcgt gccctacagt
     4261 ggactatacc cggatccggc gctcgataac cctgccttga gggcacgccc tcgcgctacc
     4321 cgcccctaag ctgccatcct catcgctaca gcccggccat gaagtcgaaa acgcggtcgt
     4381 tcgcgccggg caggccctcg tggccatagt cgggatagat caccacgctc ttgggcgcct
     4441 caatcttgtt gtaggcggcg aactgcgtcg agggcgggca gacggtgtcc atgaggccca
     4501 cgaacatcat cacctcagcg cggatgcggt gcgccaggtg ctggttatcg atgtagccga
     4561 ggcgggtgaa gaccgcctct tcgcgctcgt ggagcggatc gaagagccgg aagtaggtct
     4621 tcagctcctc gtaggcggcg acggcgagat ccatctccca gacgcgcttg tagtcgcaga
     4681 ggaaggggaa gacgggagcg gcgcgcttga tcccgggttc cagcgcggcg caggcgagcg
     4741 tgaggccacc gccctgcgag ccgccggtgg cgccgacacg cttcgcgtcc acctccggca
     4801 tggacatgac gatgcgggca agttgggcgc agtccaggta cacctgccga aagaagagct
     4861 tctccggggc gtcgtccagg cctcggatga tgtgcccgcg cagcgtattc cccttgacgc
     4921 ccccgggatc ctccgaaagc ccgccttggc cccgcacgtc gagcgcggcc acggagtagc
     4981 cctgcgagac gtaggcgagc ttatcggccc agtcgccgct gtttccggag tagccgtgga
     5041 agagaagcac ggccgggtgc ggctccggag cgttcttcgg gcgaagatac ttggcatgga
     5101 cccgcgcgcc gcctaccccg gtgaaccaga ggtggaagca ctcagcgtga tgcgtcttca
     5161 gcgggtaggg ctccagctcc acgcgggggt cgagcgctcg catctctgcc agggccctat
     5221 cccaatactc gtccatatcg gccggccgcg ggttacggcc cttatactcg tgcagttgtg
     5281 ccaatggcag atctacgagt ggcatttcgt ctctcccctg ggtgatttgt tgcttcgccg
     5341 aggcccgtgg ccctggcgcc acgggctcac acgcatattt cgcgtttggc gggcgatacc
     5401 ctgctgatca ggcgcgctgg cctcgccggt ctttagatgc ccagcatgca tcgtggaccg
     5461 aggagctcgt gaacgcgctg gaccagctcc ggagcgggct ggatgccgaa cccctgacac
     5521 tcgatggtgt agcgatctcc cggggccacc tccagctgga gcaggagacg ccggtcgcct
     5581 gggtaagcct gcaccgcgct ccgaagctcg tgcaggaagc gcgcgcgcat gtgtgcccgg
     5641 ggcacctgga tgatcaccgg gtcaccctgt ggcgtttccg gttcatccgg cacgatggtg
     5701 tctggctcgc cgcctggatg ggtaggggag atgccgttgg tgccgttgcg atagccgttg
     5761 gcgtggccgt tgccattcgc gggggcatgg agcccggcct cgccaccctc cgccggcttg
     5821 cgcacgggcc ggacgctctc cgcgatgatc tccacctggc cgcgagaggg cgcttcatct
     5881 tcgccgttct cctccttgcc cgagtccttg ctgctcacct ttccctcgat gacgacgacc
     5941 ttctccagtt gcaggtgggg agcgaactcc tggagcacgc tcggccagat gatcacctcg
     6001 acggtgccaa gccaatcctc taggccgatc accatcatcg gatcgccctt gcgtgtgttg
     6061 aggcggcgga tgcgcgagat gattccgcca acgcgcacga cctctttatc ctcgcgctcg
     6121 gctacttcct gggtggtgag cacccgcatc cgctggagcg cggggcgata ctccatcacc
     6181 gggtggtcgg tgaggtagaa cccgagcaga tcccgttcgg ctgcgagaag ctcctcccgc
     6241 gagaactcgg gcacatccgg cagcgattcc tcgggggatt cctcgggctg gcctgcatcg
     6301 ccggccagca agtccagcat agagaactgg cctacctctc gctccttgcg tgcgcgctgc
     6361 cccagggcga ttgcctggtc aagcgcggcg atgagctggg cgcggcgggc gccggtgcta
     6421 tcgaacgcgc ccgcgcggat caggcattca atcgtggccc ggttgagcgc cccggagcct
     6481 ccaatgcgcg tgcagaactc gtgcagcgag cggaaggggc cgttggcgcg ctcggcgacg
     6541 atctgctcga tctcgtctcg gcccacattc ttgatggcgg ccagcccgaa gcgcacggcg
     6601 tagcgcggtc cctcgccatc actcgccccc tcttccggct cgaacggctc aaccgagaaa
     6661 tcggcctggc tcttgttgat gtccggcggg agcaccggaa tcccgaagtg ccggcactca
     6721 tctacgtagg tgacgacttt gtccttgttt tccatgaacg ccgtcatcag ggcggcgagg
     6781 tactgtaccg ggtagttgca cttcagataa gccgtctgat acatgaggag ggcgtaggct
     6841 gccgagtggc tcttgttgaa gccgtacttg gcaaagttcg ccatcaactc atagatatgc
     6901 tcggctgtct tcttgggcac cccccgggct tccgcaccct cgatgaagag ggggcggttc
     6961 ttatccatca catcctgctt cttcttgctc atcgccttca tgagggcctc ggcctgaacg
     7021 gcgctgaagc ctcccatcac catggcgatc ttcatcacct gttcctgata gagcaggatg
     7081 ccgtaggtgt ccttgaggac cggctcgagc atggggtggg gatactccac cttggcacgg
     7141 ccatgccggc gcgcgatgaa atcctggacc atgccacttt gcagcgggcc cgggcggtag
     7201 agcgcgatga ggggggcgac atccttgaag gtacgcggct tcaggtcgcg cagaagctgg
     7261 cgcattccgg agctttccac ctggaagacg cccatggcgt cgccgttccc cagcatctgg
     7321 taggtgcgct cgtcatcgat ggggatctgg tccagcgtga gcttcttgcc cgtcgtctcc
     7381 tcgatcagat ggagggcctt gctgacaacg gtgaggtagg aaaggccgag gaagtccatc
     7441 ttcaggagcc ccacctcttc cactatccgg aagtcgaact gggtggtggg gatcggcgag
     7501 tcgccggtgc gctggagggg cacgatgttg gtgagcggct ccttggagat gacgacgccc
     7561 gcggcatggg tggatgcgtg ccggctcaac ccctcgatgc cttgcgccgt gtcgaagagg
     7621 ctgcggatct ctgggttgga ctcgtactcg ctgcgcagtt ctggaatcgt ctccaggaag
     7681 tggcgcagtg gaggcgagct gggcatggtg gggatcagct tgcagatgcg atctacgtcc
     7741 gagcggggca cctggagggc gcgagcggcg tcgcgcacgg cggctttggg gcctagcgtt
     7801 ccgaaggtga tgatctgggc cacgcaatcg gcgccatact tctccaccac atactgcaac
     7861 acctcatcgc gacggagatc ctcgaagtcc acatcgatat ccggcatctt cttgccatcg
     7921 atgcggagga agcgctcgaa catcaggtca ttctcaatcg ggtcgatgct cgagatgccg
     7981 aggaggtagg tcaccatgca gccggcggca gagcctctcg cctgggcgag gattccccgg
     8041 ctgcgcgcga agtggacgaa gtcgttcacg atgaggaagt aggcggagaa gcccttcgac
     8101 tggataacgt ccaactcaaa gcggaggcgg gcttccatct ccggcgtgaa ctcgcgatag
     8161 cggcgcggaa tgttgtcgcg gcacagcttc tccaggtagc tctcgtaggt gtagccgggg
     8221 ggcacctcgt aatgcgggag cacctgctgg aaggtgagct cgacgttgca gcgctcggcg
     8281 atctcaacgg tgttgcgcat cgcctcgggg tgatcgggaa agaggcgggc catctcttcc
     8341 acatccttca ggtagaactc cggggcgtgg aagcgcatcc gcttttcatc atcaacggtc
     8401 gtgttcgtct ggatgcacag gagaacatcg tgcgcctgcg cgtcttcctt gcgcaagtag
     8461 tggagatcgt tggtgacgat gagggggagg ttcagctcgc gcgcgagctg caggatggcc
     8521 tcgttgaccg tgcgctgctc ggggatgccg tgatcctgga gctccagata gtagcggccc
     8581 gggaagacct ccgccatcca tccggccagt tcgcgcgcct tctgcatgtt gccggcgagg
     8641 atgtgcgaag gaagctcgcc gcccaggcag gcactcgccg cgatgagccc ctcgctatac
     8701 tccgcgagga tctcgcggtc caccctcggc ttgtagtagt acccctcgag cgaggcgatg
     8761 ctcaccagct tcatcaggtt cttgtagccg gtgaggttct ccgccaggag aaccaggtgg
     8821 tactgctccc ggtcgagcac cggatcgcgg tcagtgcgcc cgcgcggggc catatacacc
     8881 tcacacccga ggatcggttt cacccccgcc ttcttgcagg cgtcatagaa ctccaccgcg
     8941 ccgtacatca cgccgtgatc ggtgagcgcc accgcgggca tgccgagctc tgccacgcgc
     9001 tccacgagct ctttgacgcg gcaggctcca tccaagagcg agaactcggt gtggttatgc
     9061 aggtgaacaa agtcagcgcc cgacatcaaa gccgatcctc tctgagcgtt tcaaatggac
     9121 ccaccgaggc atcgccgcga atacggggcc gtcaaggggg caggggccca cgcctgcgat
     9181 gcctgggccg cgatgccacc gcgctggacc cctatgttgg ggggcgccct ggcagcgtgc
     9241 cctatatgtg gcgattataa cactcgggac accggcagac aagccgacag aatgttctgg
     9301 tggcgttcag cctggatgcg agggagacga ttcggcacgt gatttgctgt agggaagggg
     9361 agccttcacg gcgcggcaag ggggaaggcg tcgaaggtcg tgggggctcg cgttccggcc
     9421 ggccagggag ccggcctcca gatagagtgc ggctccggtg gggggctccg caaaaggagg
     9481 cattccgacc gtgcgatggg ttagcccatt accggagccg cgcatctacc tgttacccag
     9541 gaatcccctg ggccaaacgc gcgtccccgg gtcatgcgcg aggcagcaac ccggcctcgt
     9601 gcgtgcgctg gaatggatgg ttagcccggg gtggatctgg gcatagatca gggcgagttc
     9661 accaatcgcc cggagaggtg cgcggaggta ggcgtggagg atctctttgc ggtcagggtg
     9721 agtggcaacg cagtggtgga gtgtcagggc gagatggata tctgcgcgga ggcgcccctg
     9781 cgggaggcgc tcgcgcgagc aatgcacgat catccgacgc aggtacaggt ggacctctcg
     9841 caggtgcgct acatcgactc tgcgtgtatc gccgtgctcc tgcgtgccag cgataccctg
     9901 cgccagcagg gcgggagcct ggtgctgacc gccgcaagcc ccgtggtatg ccgcgctctc
     9961 tccctcctcg gcctggatca gcggcttcgg cccggcggca tcggcgaacc gccccgcggc
    10021 tcctcatagc tgggcacaag tcgcaggcct ttcgtagcct ccttcttccc cccgcgcacg
    10081 acgcggaatc gcccttctcc ccgcgagagc ggccgcagtg aatcgctccc cacggccggt
    10141 ctgcgccgcc catctccctc catctttgca gatgcggtgg ccagcgcgga agcaccgtgg
    10201 cagcctcggg ctgctgcaga gcaggtggga tgctcacgct gccttatgcc cctcgccagg
    10261 acgcaggaga gcgccctccc ggcggcgaag cttggccgca gtgaggaggg aatagagccg
    10321 catgatgcag agcgtgttga cgaacctgct ggaggatgaa tggcagaagg ccgatccgga
    10381 gacgtgggcc aagcgccggg ccgcgatcca tgccgcgttt acggagctgt tgggtgaggg
    10441 cgtggtggcg cccccggagg atcgtgacct gcaatggcat ggcgaagagc gcatcgatgg
    10501 gttgcgcatc cggaagctga gcttcctcgc cgagccggat gaccgcatcc ccgcctatct
    10561 ggtgatgccg gaggagcttt cggcgcccgc gcctgccgtc ctttgcctgc atggcaccac
    10621 ggtggatgcc aaggaggcgt gcctcggacg agggagtcat ccaggcggct ccaagggcac
    10681 agcggttgac ctggcccggc gtggcttcat caccctggcg ccggaccact tcaacgcggg
    10741 cgaacgcctg aagcccggcg agaagcccta cgatagcggg cctctctacg cgcgccatcc
    10801 cggttggagc gacatgggga aggcgatcta cgaccaccgt ctctgtgtcg atctgcttca
    10861 gacgctgccg gaggtggatg gtgaccgcat cggctgcatc ggccactcgc tcggcggcta
    10921 ctgcaccctt ttcctcgctg ctatggacga gcgcatccgt gcgggcgtct caagctgcgg
    10981 cgtgacggtg tgggccgccg atccgaaccg cctgaactgg tcgcgtgacc agcccgggcg
    11041 gtatgtccac ttcccgaagc ttcgcgagta ttggaactcg ggccggcagg cgcctgtgga
    11101 gttccacgag atcatggccc ttgttgcccc ccgcgccttt ctcaacatca gcgcggtcgg
    11161 gaacgatgtc tgtttccccg tcttcgcgcc ttttgctgag ctctacgtgc aggtggagag
    11221 cgtctacaag ctcctcggtg ccgaggggaa gtttgccgct cacttccact cttgcggcca
    11281 ctctttcggc ccgtcggcgc gcgctctcgc ctacgcctgg ctggccgaac agctggagct
    11341 gtagacgccg atctcggacc gggtttcagg agcgccctcc tgaaacccgg gtcgctcccc
    11401 ggtcactgag agggcgtccc tggggtgtgc cctctcagga atcgaggtcg gcatagctgc
    11461 cggtgcgcac gcgagcgcgc ccggtcaggc ggagctctcc gtggtccggc tccaccgtgc
    11521 ggaggtagac cggcaggcgc gcgctgctga gatcgaccag gggattcacc tgggatgcca
    11581 acagcttggc ggcgaactcc ggaacctcga cgcccaccac gcgaatctta ctcgcgtcga
    11641 gaatcaggcg cccctcgcca tcaatcttca gcgtgccctc caggagaaaa tcgggggtga
    11701 ccggcaactt cacgccggga agatcggcgc tcccgcggag gcgcgcgccc tccggagtga
    11761 tgctgagctt caggccgcgt atcagggagg atcgctcgtg aagctgcgca ttcagatcct
    11821 cctggaggag gacggcggag aagtcggc
//
LOCUS       DAOSCV010000015        11838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3638_length_11838_cov_3.261733, whole genome shotgun sequence.
ACCESSION   DAOSCV010000015 DAOSCV010000000
VERSION     DAOSCV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11838
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3638_length_11838_cov_3.261733"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PLY56_00815"
     CDS             complement(<1..519)
                     /locus_tag="PLY56_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ20050.1"
                     /translation="MERKEYSMMDVAPTVSAVLGLPVPAQAKGKPIAEMAQDLAGVPR
                     VAVLAPDALGEFAWSLWKQEMPFLRSLHARRSILLRSVMPSITPVNFATMVTGTDLEG
                     HGVRSRELPFACETLFDVVRRAGGKSAGIGLDNYTGTMLLARHADIAGNAGRGTDDDV
                     ADLILRIAAEQQP"
     gene            complement(640..1305)
                     /locus_tag="PLY56_00820"
     CDS             complement(640..1305)
                     /locus_tag="PLY56_00820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ20051.1"
                     /translation="MKNATRASILKAVAILLYAAVVLPFLRSGVPGSYDRYLVYNYII
                     LFFVPLLLITAVFRDQPEEYAVGPGDWRAALRFFVLLYVPTLIGLAIAARWPAFQAYY
                     PINKMAGYGRQEFLFWEVAYNGFYMFSWEFFFRGFLLFGLRPALGNGSLHLQAIVFGV
                     MHWGKPMPEFFASFLTGYVLGIVALRSRTFLPTFALHAACAASFDFLVLAWAGRLGPL
                     LGL"
     gene            complement(1316..1780)
                     /locus_tag="PLY56_00825"
     CDS             complement(1316..1780)
                     /locus_tag="PLY56_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20052.1"
                     /translation="MKQVVWLLEDSLGPIDPALRALPEAPAVYIYDEDDLRAHPTSFK
                     RLFFIYESLVETFAGRSAPCEIRRGNLVREMIDFCRKYGAEEIHVTRSPSPAYRLWTE
                     QLRHTLVVVEYAPETLVKWSGRPPRRFLELWEQAYPQLLPEEKPGAGEDEES"
     gene            complement(1777..2544)
                     /locus_tag="PLY56_00830"
     CDS             complement(1777..2544)
                     /locus_tag="PLY56_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HOJ20053.1"
                     /translation="MIIPHEPKEREALRQHAAEALKGLYQGEAVASPWVGGRQAGLRR
                     LEALDIDRYNREHNGAHQEATSGLSPYIRHGLITLREARDSVVSRFGAAASRNFVIQL
                     LWRVYWYLRARQRGVASLVDAAAGATEIPVPQVASSPLCIDEALRCLRATGFLAYHPR
                     LWVASYFLHWLGIPWRQGYRLFQEHLIDADPIVNALSWRWVLGEETGRPYFFTRAQVE
                     RCTGGQLCARCTIYRCPFEGTPEAVEERARQPRRLFR"
     gene            3222..4406
                     /gene="ugpC"
                     /locus_tag="PLY56_00835"
     CDS             3222..4406
                     /gene="ugpC"
                     /locus_tag="PLY56_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOJ20054.1"
                     /translation="MAGVTLRNVTKRFKNVVAVNNVNLEIRDKEFLVLVGPSGCGKTT
                     ALRMVAGLEEITEGEILIGDRVVNDVSPKDRDIAMVFQNYALYPHMSVFDNMAFGLRL
                     RMLPGTIGRLTRRAETRAINDQIRKRVETVAEMLGLTQLLDRKPKQLSGGQRQRVALG
                     RAIVREPKVFLMDEPLSNLDAKLRVQTRAELIKLHRQLGITSIYVTHDQVEAMTMGER
                     IAVMRDGVVQQVDTPLKLFNEPANLFVAGFIGSPAMNFVPSLLEGDAGGVYVNAGSFR
                     VRLPDCMVKSSSKIDAYIGKEVIFGVRPQHIYDKYKSLVPATADNMIRCNVDVSEPMG
                     SVVTLYLTSGQHFMVAEVDAETRAVDGAPVDLVLDMRKTHLFDKETERAIFDEGTVRD
                     AD"
     gene            4598..4909
                     /locus_tag="PLY56_00840"
     CDS             4598..4909
                     /locus_tag="PLY56_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020735304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOJ20055.1"
                     /translation="MAKRIFVGGLSYNTTSDGLREAFEAIGEVTEAAIVTDRETGRSR
                     GFGFVEMATDELADAAIAQLNGTNLDGRTLTVNEARERAPRGGGGRGGYGGGYGGGGR
                     W"
     gene            complement(4981..5982)
                     /locus_tag="PLY56_00845"
     CDS             complement(4981..5982)
                     /locus_tag="PLY56_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ20056.1"
                     /translation="MMSMPAGNERATPLPFDLVREELRQVDELLLQETWSAVSIVRDI
                     SHHILGAGGKRLRPALLLTTARAAGYQGDWAIRLALCMELLHTATLMHDDVLDHSDWR
                     RGRVTAHRRWGQSASILTGDAMFARAMRILVDYGSLPILNAVTSAVIRVCEGEVMEIA
                     LHGDLDIAPETYLRVIDAKTAALISVCCQTGGMLGQTTPAQIEALSTFGFHLGMAFQM
                     VDDLLDFTAKRERWGRPAGEDLKEGRITLPVIYTLQRATPADRERLRTILNTPSAAVE
                     HMDEVLSLLHRYGGFEAARADAIAHTRAARESLLALPASAARDALAALADFVVARDQ"
     gene            6188..6676
                     /gene="greA"
                     /locus_tag="PLY56_00850"
     CDS             6188..6676
                     /gene="greA"
                     /locus_tag="PLY56_00850"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ20057.1"
                     /translation="MAVEREKEIVLTPEGFRRYEEELNRLTTVTRQEVRARLRDTKPT
                     SDEGEDAAYDSAKIELAFVEGRIQELREILTRARILAPEDIRTDEAHLGSRVRVTNLS
                     TGRELTLKLVSPLEAAPERGQISTESPVGEAILGRRPGDTVDVRTPSGTVQYRIEEIR
                     AE"
     gene            6682..8184
                     /gene="lysS"
                     /locus_tag="PLY56_00855"
     CDS             6682..8184
                     /gene="lysS"
                     /locus_tag="PLY56_00855"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ20058.1"
                     /translation="MDQQVSNVDDLFEQRLAKLQALRDRGIDPYRPERFDRTHLAAEI
                     LENFDALAESAEPVAVCGRVVSMRVMGKAAFLHLLDVSGRIQVYLKLDLIGAEQFALL
                     EFIDIGDFLGVHGITFRTRTGEITIQAERFQILSKALRPLPIGKQKGDEQWYGLQDVE
                     QRYRQRYLDLIVNPWARKPLLQRVQIVREVRRFLEDRGFLEVETPVLQPLAGGAAARP
                     FLTHHNALDTDLHLRISLELYLKRLVIGNLERVFELSRVFRNEGISPRHNPEFTLLEV
                     YQAYTDLEGMMELTEQLISHVVTSVHGGPRFTFAGQEIDVTAPWRRVSMMELIREYAG
                     ADREEFTTLESARAVGRRLGLPMDEEYTIGGIIDKVFEKACEPRLVQPTFVTDFPLEL
                     SPLAKKRADDPTLTRRFEAFIGGQEVVNAFSELNDPLDQRERFEAQVAMAAAGDEEAH
                     PYDEDFIRALEYGMPPTGGLGIGIDRLAALVTDQPSLRDVIFFPQLRPER"
     gene            complement(8225..9187)
                     /locus_tag="PLY56_00860"
     CDS             complement(8225..9187)
                     /locus_tag="PLY56_00860"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ20059.1"
                     /translation="MNTDEERTIISSTVAQPPAGEATIVFAQDMADRTQMSVSQECPV
                     CHTTNPPGEVYCAECGFLLSGPPPESVEEASAPVACLVASDGREFALNPGRNSIGRAN
                     ADVLLTDPSISRTHAAITLEEGRCLVEDLGSTNGTRVNGENLVPHQPQALRDGDEIAF
                     GGLSLRVALPEGYAAAPATAAEEASPLAAEPSPARFIDEESGVEYPIPTGTLTIGRRS
                     ANDIAFPTDPYVSGRHAQVSFDGETLTLTDIGSTNGTVLNDDRLAPNTPVPLQDGDLV
                     QMGQQRLRVRLAPIPEEEGEVVDEAAEPLAADYPPPGDETPGLW"
     gene            complement(9386..10786)
                     /locus_tag="PLY56_00865"
     CDS             complement(9386..10786)
                     /locus_tag="PLY56_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ20060.1"
                     /translation="METTRMLSPGDDDRTQAITPGIDQTQVVSDPNRTQYAPPPQAGD
                     ADRTHYAAPPAQGVQLHCLVENAYAPAVRNTRSHLLVQVQAPETAYGRRLPINLCLCI
                     DRSGSMEGEPLDYVKRACAHVVDLLDPNDVLSVVTFAEEIEVVMPARRVVNRELIKQH
                     ILRIDTGNTTNLYDGILVAGRQVASVQSPAYLNRILVLTDGEPTTGIKDFQSIVGQVA
                     ELKSRGITVTALGFGPDYNEELMAGIARRSGGNYYFISRPELIPEVFRKELETLMTVV
                     AKNLRLRLNLTRWTQMRQVYAGQPAFGPRTVDLSLVDLERGALITALAEVELQRRPAG
                     VYRVAQAVLSYDDVTTGRTQQLTADAVVEFVTDESRVPAGINPVVLREVEVAQASRHL
                     ERTVMGMRTQALSTGAAIAELERTQALLAAQGRIDQAQEVTQAIAAIRQGDTSTEAEK
                     TLMGTILDLEQGKRKA"
     gene            11329..>11838
                     /locus_tag="PLY56_00870"
     CDS             11329..>11838
                     /locus_tag="PLY56_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ20061.1"
                     /translation="MIDFQNVSVAYSNGVQALSDIDLHFEKGEFVFLVGPTGTGKSTL
                     LKLIYRGVLPNKGRVTVMGEDVTTLPPSRVPHLRRRLGVVFQDFQLLPQKTVYENVAY
                     ALHAIGVPYREIRRRVCTAVDLVGLGRRADAYPSQLSGGEQQRCCIARAIVNNPPILL
                     ADEPTGNLDS"
BASE COUNT         2060 a         3722 c         3961 g         2095 t
ORIGIN      
        1 gggctgctgc tcggcggcga tgcggaggat gaggtcggcc acgtcgtcat ccgtgccgcg
       61 gcccgcgttg cccgcgatat cagcgtgccg cgccaggagc atcgtgccgg tatagttgtc
      121 caggccgatg ccggcgctct tgccgcccgc gcgccgaacc acgtcaaaga gcgtctcgca
      181 cgcgaagggc agctcgcgac tgcgcacgcc atgcccctcc aggtcagtgc cggtgaccat
      241 cgtggcgaag ttcaccgggg tgatggacgg catcacggag cggaggagga tgcttcgccg
      301 cgcgtggagc gagcggagga acggcatctc ttgcttccaa aggctccagg cgaactcgcc
      361 cagcgcgtcc ggcgccaaaa cggccacgcg gggcaccccg gccaggtcct gagccatttc
      421 ggcaatcggc ttcccctttg cctgcgcggg cactggaagc cccaaaaccg cgctcaccgt
      481 cggcgccacg tccatcatcg agtactcttt gcgttccatt gaccattctc ccatccaccc
      541 cgggcgaatc gcgctcgggc acgccacaat ttggcacacg ggttcgccgc tgggcgcgcg
      601 atcccctctt ccaaatcgag ggcatgagag cttgctaggt cacaatccga gcagggggcc
      661 gagcctgcca gcccaggcga ggacgaggaa gtcgaaggag gcggcgcagg ccgcgtgcag
      721 ggcgaaggtt ggaaggaagg tgcgcgagcg cagagccacg atcccgagga cataccccgt
      781 gaggaacgag gcgaagaact ccggcatcgg cttcccccag tgcatgacgc caaagacgat
      841 tgcctggagg tgcaacgagc cgttgcccag cgccggacgg aggccgaaga ggaggaaccc
      901 gcggaagaaa aactcccacg agaacatgta gaagccgttg taggccactt cccagaaaag
      961 gaactcctgc ctgccgtagc cggccatctt gttgatcggg tagtaggcct ggaacgcggg
     1021 ccagcgcgcg gcaatcgcca ggccaatcag cgtgggcacg tagaggagca cgaaaaaccg
     1081 gagcgccgcc ctccagtcgc ccgggcccac ggcgtactcc tccggctggt cgcgaaacac
     1141 cgctgtaatc aggagcagcg gcacgaagaa aaggatgatg tagttgtaga ccaggtagcg
     1201 gtcataggag ccgggcaccc cggatcgcag gaagggcagg acgaccgctg cgtagaggag
     1261 gatggcaact gccttgagga tgctggcacg agtggcgttt ttcatcggat cgttctcagc
     1321 tctcctcgtc ctcgcccgcc ccgggcttct cctccggcaa cagctgcgga tacgcctgct
     1381 cccaaagctc gaggaaccgg cgcggcggcc ggcccgacca cttcaccagc gtctccggag
     1441 catactcgac cacgacgagg gtgtgccgga gctgctcggt ccagagccgg taggctggcg
     1501 acggcgagcg cgtgacgtgg atctcctcgg cgccgtactt gcggcagaag tcgatcatct
     1561 cgcgcaccag gttcccacgg cggatctcgc acggcgcgct ccgtccggcg aacgtctcga
     1621 ccagcgattc gtagatgaag aagagacgct tgaacgaggt ggggtgcgcg cgcaggtcat
     1681 cctcgtcata gatatagacc gctggcgcct cgggcagcgc cctcagcgcg ggatcgatcg
     1741 gcccgagcga gtcttcgagc agccacacga cctgtttcac cggaacagcc tccttggctg
     1801 gcgtgcgcgc tcctccaccg cctccggcgt tccttcgaac gggcaccggt agatggtgca
     1861 ccgcgcgcag agctggccgc cggtgcatcg ctccacctgg gcccgggtga agaaataggg
     1921 tcggcccgtc tcctcgccga ggacccagcg ccaggagagc gcgttcacaa tcgggtcggc
     1981 atcgatcagg tgttcctgga aaaggcgata gccctggcgc caggggatcc ccagccagtg
     2041 gaggaagtag gaggccaccc aaagccgcgg gtggtaggcg aggaacccgg tggcgcgcaa
     2101 gcaacgcagt gcctcgtcga tgcagagcgg gctgctcgcg acttgcggca ccgggatctc
     2161 ggtcgcgccc gcggcggcat cgaccaggct ggccacgccc cgctggcgcg cgcgcaggta
     2221 ccagtagaca cgccagagga gctggatcac gaagttgcgt gacgccgcgg cgccgaagcg
     2281 gctgaccacg ctatcgcgcg cctcgcgtag agtgatgagc ccgtgacgga tgtagggcga
     2341 gagcccggac gtcgcctcct ggtgagcgcc attgtgctcc cggttgtagc ggtcaatatc
     2401 cagcgcttcc aggcggcgca agcccgcttg gcgtccgcct acccaggggc ttgccacggc
     2461 ctctccctgg taaagacctt ttaacgcctc ggcggcgtgt tgccgcagcg cctctctctc
     2521 cttcggctcg tgtggaatga tcatcgccag cggctctcag tatgccacac accctcccct
     2581 ttgtcaacat ccgggcgaat ggcgtgtgcg cgaggcggcg agggcgcctc ggacgcatct
     2641 gcggcaggca gcgcagggta ctcccgaacg ttccggttca gcgttgtggc atccgtgacg
     2701 agcggctcgc gctgggcaat gaaggcgccc tgccttttcg cggtggggat tccgagatgg
     2761 gggttgccag ctatccccgg ctaagccacc aaagtggggt ccggcgtccg gcacggcgcc
     2821 gctccagccc ggtgccatcc ggaggtggcg atggctggaa ccgggcatcg gaggcgggtc
     2881 attccaccgc gccgaggctc gctctccggc ctgggcacca cccgtgctgg attccctctc
     2941 tcggaaaccc tgcttcgtgc ctgcgtttcg acgccttgac aaaagctgca ggcgttcata
     3001 taatacatcg gagcaagcgt ggcgacatat gccaccgctc ctaccgcgca tcgccactgg
     3061 cagattcgca ccgcgggcaa ggaaaggccc cacgtccttt catccttgag aggttcctgc
     3121 gtttgcggcc gcttggggtg cgcgagatat ggatgtctag agcgatttgc ggtctgccgt
     3181 ggcgggatga ccgcgcggcg cctttgagga ggctatcact gatggcagga gttaccctgc
     3241 gcaatgttac caagcggttc aagaacgtcg tagccgtcaa caatgtgaac ctcgagatcc
     3301 gggacaagga gttcctggtg ctcgtgggtc cttctggatg cggcaagacg acggccctgc
     3361 gcatggttgc gggtctcgag gagatcaccg aaggagagat cctgattggc gacagggtag
     3421 tgaacgacgt ctctcccaag gaccgcgaca tcgccatggt gttccagaac tacgcgctct
     3481 acccccacat gagcgttttc gataatatgg cattcggact gcgcctgcgc atgctgcccg
     3541 gcacgattgg gcggctcacc cggcgggccg aaacgcgcgc tatcaacgat cagatccgca
     3601 agcgcgtgga gacggtggcg gagatgctgg ggttgactca gctgttggac cgcaagccga
     3661 agcagctttc gggtggccag cgccagcgcg tggccctggg ccgggccatt gtccgagagc
     3721 ccaaagtctt cctgatggat gaacccctct ccaacctcga tgccaagttg cgcgtgcaga
     3781 cgcgtgccga gctgatcaag ctccatcgcc agctcggcat caccagcatc tacgtcacgc
     3841 acgaccaggt tgaggcgatg acgatgggcg agcgcatcgc cgtgatgagg gatggtgtgg
     3901 tccagcaggt ggacacgccg ttgaaactgt ttaacgagcc cgcgaacctc ttcgtcgctg
     3961 ggttcatcgg tagcccggcg atgaacttcg tcccgtcgct cctcgaagga gacgccgggg
     4021 gggtctacgt caacgcgggc agcttccggg tacggcttcc cgattgcatg gtgaagtcga
     4081 gctcgaagat cgacgcctac atcggcaaag aggtgatctt cggcgtgcgc ccgcagcaca
     4141 tctatgataa gtataagagc ctggtgccgg ccacggccga caacatgatc cgctgcaacg
     4201 tggatgtctc ggagccgatg ggctcggtcg tcacgctcta cctcaccagt gggcaacact
     4261 tcatggtggc ggaggtggac gcggagaccc gggccgtgga tggcgcaccg gtcgatctgg
     4321 tcctcgacat gaggaagacg cacctgtttg acaaggagac agagcgcgcc atcttcgacg
     4381 aggggaccgt gcgcgacgcc gactaatccg caggaagtcc agaagcttgc ctttctgggc
     4441 atgatgcgat ataattcgcg tgttcggggc cgctggccac cctcaggcgg cctacgagaa
     4501 gcgtgtagtc cgttttggtg cggcgatggt tcttccgacc cgagtagcgc cttctggaga
     4561 cgcacgtgca actaatgagg gggagaggag ccatcccatg gcaaaacgga tctttgtggg
     4621 cggtttgtcc tacaacacca cttctgacgg actgcgtgaa gcgttcgagg cgatcggcga
     4681 ggtgaccgaa gcggcgatcg tcactgatcg cgagacgggc cgcagccgcg gcttcggctt
     4741 cgtcgagatg gccacggacg aactggcgga tgccgccatt gcgcagctca atggtacgaa
     4801 ccttgatggc cgcaccctga ccgtgaatga ggcccgcgag cgcgccccgc gcggcggcgg
     4861 aggccgtggc ggttacggtg gtggctacgg cggcggaggc cgctggtagg ccgatcgatc
     4921 cacgccaact gagatcatag cacggggctg ccacccgatt gggtggcagc cccgtttgac
     4981 tcattggtca cgtgcgacaa caaaatcggc aagcgcggca agtgcatcgc gagcggcgga
     5041 tgccggcaag gccaacaggc tctcgcgcgc ggcgcgggtg tgcgcaatgg catcggcgcg
     5101 cgccgcctcg aagcctccgt agcggtgcaa gaggctcaac acctcgtcca tgtgctcgac
     5161 agcagccgac ggggtgttta gaatggtgcg caggcgctcg cggtccgcgg gagtggcgcg
     5221 ctgcagcgtg tagatgaccg gcagggtgat gcggccttcc ttcagatcct cgccagctgg
     5281 tcggccccaa cgctcgcgtt tcgcggtgaa gtcgagcagg tcatctacca tctggaaagc
     5341 catgccgagg tggaagccaa aggtgctgag cgcctctatc tgggccggag tggtttgccc
     5401 gagcatccca ccggtctggc agcagacgga gatcagcgca gccgtcttgg cgtcgatgac
     5461 gcgcaggtag gtttccgggg cgatatccag gtcgccatgg agggcaatct ccatgacttc
     5521 gccttcgcag acccgaatca cggcgctggt gaccgcgttc aagataggca ggctgccgta
     5581 gtcgacgagg atgcgcatcg cgcgcgcgaa catggcatcg cccgtgagga tcgacgcgct
     5641 ctgcccccat cgccggtgcg cggtgacgcg cccccgacgc cagtcagagt ggtcgagcac
     5701 gtcgtcatgc atcagcgtgg cggtatggag cagctccatg cagagggcaa ggcggatggc
     5761 ccaatccccc tggtagcccg cggcacgtgc ggtagtcaac aacaacgccg ggcggagtcg
     5821 cttccccccg gcgcccaaaa tgtgatgcga gatatcacga acgatcgata ccgccgacca
     5881 cgtttcctgg agcaagagtt catctacctg acggagctcc tcacgcacca ggtcaaaggg
     5941 aagtggcgtc gctcgctcgt tccccgcagg catgctcatc attcgctgaa acctgtgggt
     6001 ccttttagtt tagcgtgagt ataccatagg ggcgggggct agtcaagagg agttgttttt
     6061 gcggcggatg tggcgttgac agcggacagc cttcatgata taatgacgcc ggtcaggagc
     6121 actgatcgcg ccgtttgagc gtggtcagtg cttgtcgttt atgtaccgca cgttggggga
     6181 tcgcgccgtg gcagtagaaa gagagaagga gatcgttctg actccggagg ggtttcggcg
     6241 ctacgaggag gagctaaacc gcctgacgac cgtgacccgc caggaggttc gtgctcgcct
     6301 tcgtgatacc aaaccgacga gcgacgaggg cgaggatgcc gcctatgaca gtgctaagat
     6361 tgaactggcg tttgtcgagg gccgaatcca ggagctgcgc gagatcctga cacgcgcccg
     6421 catcctggcg ccagaggata tccgcaccga tgaagcacat ctgggctccc gcgttcgcgt
     6481 gaccaacctc tctacgggac gtgagctcac cctgaaactg gtcagcccct tggaagccgc
     6541 gccggaacga ggccagatct cgaccgagtc gcctgttggc gaggcgatcc tgggccgacg
     6601 ccccggcgac acagtggatg tgcgcacgcc ctccggaacc gtccagtacc gtatcgagga
     6661 gattcgtgct gagtaggaat aatggaccag caagtatcta acgttgatga cctctttgaa
     6721 cagcggcttg ccaagctgca agcgttgcgc gaccggggca ttgatcccta tcgcccggag
     6781 cgcttcgatc gcacccatct ggcagccgag atcttagaga actttgatgc ccttgcggag
     6841 agtgccgagc ccgtggcggt ctgcggccgc gtggtctcca tgcgcgtcat ggggaaggca
     6901 gccttcctcc atctgctcga tgtgtccggg cgcattcagg tctacctgaa gctagatctc
     6961 atcggcgccg agcagttcgc gctcttggag tttatcgaca ttggcgattt tctcggggtg
     7021 catggcatta ccttccgcac gcgcaccggc gagatcacca ttcaggcgga gcggttccag
     7081 atcctctcca aggcgcttcg cccgctcccc atcggcaagc agaagggcga cgagcagtgg
     7141 tacggccttc aggatgtgga gcagcgctat cggcagcgct atctcgacct gatcgtcaac
     7201 ccgtgggcac gcaagccatt gcttcagcgc gtgcagattg ttcgcgaagt gcgccgcttc
     7261 ctggaggacc gcggcttcct ggaggtggag accccggtgc tccagcctct tgccggcggc
     7321 gcggcggctc ggcccttcct gacgcatcat aacgccctgg ataccgacct ccacctccgc
     7381 atctcgctcg agctctacct caagcgcctg gtcatcggca acctggagag agtcttcgag
     7441 ctgagccgcg tcttccgaaa cgagggaatc tccccgcgtc acaatccgga gttcaccctc
     7501 ctggaagtct atcaggccta caccgacctg gaaggcatga tggagctgac cgagcagctc
     7561 atcagccacg tggtgaccag cgttcacggc ggcccacggt tcacgtttgc cggccaggag
     7621 atcgatgtga ccgcgccatg gcggcgggtg agcatgatgg agctgatccg ggagtacgcg
     7681 ggcgcggacc gcgaggagtt cacgaccctc gagtccgcgc gggctgttgg gcgccggctt
     7741 ggcctgccga tggatgagga gtacaccatc ggtggcatca tcgacaaggt gttcgagaag
     7801 gcgtgcgagc cccgtctggt gcagcctacg ttcgtcaccg atttcccgct ggagctctcg
     7861 ccactcgcca agaagcgcgc cgatgatccc acgttgacgc ggcggttcga ggcgttcatc
     7921 ggcgggcagg aagtggtcaa cgccttctct gagctgaatg accccctcga ccagcgcgag
     7981 cgcttcgagg cacaggtggc gatggccgcg gccggcgacg aggaagccca tccctacgac
     8041 gaggatttca tccgcgctct cgagtatggc atgcctccca ccggcggcct cggcatcggg
     8101 atcgatcggc tggccgcgct ggtgacggac cagccctcgc tccgcgatgt gatcttcttc
     8161 ccgcagctcc gcccggagcg atagccgcat ccgggcgggc gcgtccccac cacggggggc
     8221 cttactacca gagcccgggt gtctcgtctc ctggcggcgg gtaatctgcc gccaggggtt
     8281 cggcggcttc gtctacgacc tcaccctctt cctccgggat gggcgccagc cggacgcgga
     8341 ggcgctgctg gcccatctgc accaggtcgc catcttgcag gggcaccggc gtgttgggtg
     8401 ccaggcggtc gtcattgagc accgtgccgt tggtgctgcc gatatcggtg agcgtgagtg
     8461 tctcgccgtc gaagctcacc tgcgcgtgcc ggccagagac gtagggatcg gtggggaagg
     8521 cgatgtcgtt ggcggagcgc cggcctatcg tcagggtgcc ggtcgggatg ggatactcga
     8581 cgccgctctc ctcatcgatg aaccgcgccg ggctgggctc ggctgcaagg ggtgatgctt
     8641 cctcggcggc ggtggctggc gcagcagcat acccttccgg gagcgccacg cgcaggctta
     8701 gtccgccgaa ggcgatctcg tcaccgtcgc gaagcgcctg cggttggtgg ggcaccagat
     8761 tctcgccgtt gacacgcgtg ccgttggtgc tcccgagatc ctccacgaga cagcggccct
     8821 cctccagagt gatcgccgcg tgcgtccggg agatggaagg gtcggtgagg aggacgtcgg
     8881 cgttggcgcg gccgatgctg ttgcgtccgg ggttgagggc gaactcgcgg ccatcgctcg
     8941 ccaccaggca ggccaccggc gcggaggcct cctccacgct ctctggaggc gggccgctca
     9001 ggaggaagcc gcactcggcg cagtagacct cgccgggcgg gttcgtggtg tggcagaccg
     9061 ggcactcctg cgagacggac atctgggtgc gatccgccat atcctgcgcg aagacgatgg
     9121 tggcctcgcc ggcgggcggt tgtgccaccg tgctgctgat tatggtgcgc tcttcgtctg
     9181 tgttcatggt ctcccgttcc cgctaggcca aggtggcgac agggtgaagg gggcgacggc
     9241 ctccacggtt cgagagccgg ttgcggagca gctcgaccag aaaaccggtc gcgcctgcga
     9301 atccggaccg ctccgtcacc tccctgctcg catcacgcca cgaaacgatc acgtagcctc
     9361 aaagggcggg ctcgcggcga gcgtgttacg ctttgcgctt tccctgctcc aggtcgagga
     9421 tggttcccat cagcgtcttc tcggcctccg tgctggtatc gccctggcgg atggcggcaa
     9481 tcgcctgggt gacctcctga gcctggtcga tccgcccctg ggcagcgagc agggcttgcg
     9541 tgcgctccag ctccgcgatg gcggcgccag tggagagggc ctgcgtgcgc attcccatca
     9601 ccgtgcgttc caggtggcgc gacgcctggg cgacctccac ctcccgcaac accaccgggt
     9661 tgatgcctgc gggcacgcgg ctctcgtcgg tgacgaactc cactacagcg tcggccgtca
     9721 gttgctgggt gcgcccagtg gtcacgtcat cgtagctgag cacagcctgc gccacgcgat
     9781 agacacccgc cggacggcgc tgcagctcta cttccgcgag cgccgtgatc agcgcgcctc
     9841 gctccaggtc caccaggctc agatcgacgg tgcgtggtcc gaaagcgggc tggccggcat
     9901 acacctggcg catctgggtc cagcgcgtca ggttgagccg gaggcgcaga ttcttggcga
     9961 ccacggtcat cagcgtttcg agctctttgc ggaacacctc cgggatcagc tcgggccgcg
    10021 agatgaagta gtagttgccg ccgctgcgcc gggcgatgcc cgccatcagc tcctcattgt
    10081 agtcggggcc aaagccaagg gcggtgaccg ttatcccacg gcttttcagc tcggccacct
    10141 ggcccacaat cgattggaaa tccttgatgc cggtggttgg ctcgccgtcg gtaagcacca
    10201 ggatgcggtt gagataggcg ggcgattgga cggacgctac ctgccgtccg gccaccagga
    10261 tcccatcata gagattggta gtgttgcccg tatcgattcg caggatgtgc tgtttgatga
    10321 gctcccggtt caccacgcgg cgcgcgggca tcaccacctc gatctcctcg gcaaaagtga
    10381 ccacagagag gacatcgttg ggatcgagaa ggtccaccac atgggcgcaa gcgcgcttga
    10441 cgtagtccag cggttcaccc tccatcgagc ccgagcggtc aatgcagagg cagaggttga
    10501 tgggcagacg ccggccgtag gcggtctcgg gcgcctgaac ctgaacgagg aggtgcgagc
    10561 gcgtgttgcg cacggcgggg gcgtaagcat tctctacgag gcagtgaagc tgcacgccct
    10621 gggctggtgg tgccgcgtag tgcgtgcggt ccgcatcgcc cgcctgtggc ggcggcgcgt
    10681 actgcgtgcg gttgggatcg gacacaacct gggtctggtc gatgcccggt gtgatggcct
    10741 gggtgcggtc gtcatcgccg ggcgagagca tgcgggtggt ctccatcggt cgtttccccc
    10801 ctctccggca ttatacgaaa ccgctcctga gatgtcaaac aagcaaccgg cttggccggc
    10861 gcgcttggga ttccgagaca agtgaggtcc gggcgattgc ctgcgttctc ccgcccgcgc
    10921 ccttcctccg ataccacgga ggaagggcgc ggagatggat gggccgggta cgcgcagcgc
    10981 gcgatccatt cgggctcctc tgcgaccgcc cacggtagca ccccttccct ggggccggta
    11041 tctggccggc gggcagggaa ggggccgggg gttaggaaag ccgtggatgg gccgctggct
    11101 cgcattgaca accggaggcg gcaccgatat aatgtgggta ctgggtttat tctcaggatc
    11161 tgtcagccat cataccgtgg agggggcaac ccccgcgcga gcgtctccgg gaagaaggtc
    11221 tggagtaatc gcgtccggct accagtccgc tagtcatggc agcgtgtctc tcactctctc
    11281 atgcctgcga ggcgaagtgt ggccgagtgc gcgtgggggt ggaccgccat gatcgatttc
    11341 cagaacgtat ccgtcgccta ctccaacgga gtgcaggcgc tgagcgacat tgacctgcac
    11401 tttgagaagg gggagttcgt cttcctggtg gggccgactg gcaccggcaa gtcgaccctc
    11461 ctgaagctga tctatcgggg agttctgccg aacaagggcc gcgtcaccgt tatgggcgag
    11521 gacgtgacga ccctgcctcc cagccgggtg ccccacctgc gccgccgcct gggcgttgtt
    11581 tttcaggact tccagctgct gcctcagaag acggtttacg aaaacgtcgc ctatgccctg
    11641 cacgcgattg gagttcccta tcgcgagatt cgccgccgtg tctgcaccgc cgtggacttg
    11701 gtcggcctgg gccgcagggc agatgcctat ccctcgcagc tttccggggg ggagcagcag
    11761 cggtgctgca tcgcccgtgc gattgtcaac aacccgccca tcttgctggc agatgagccg
    11821 accggaaatc tggattct
//
LOCUS       DAOSCV010000016        11823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3643_length_11823_cov_3.508923, whole genome shotgun sequence.
ACCESSION   DAOSCV010000016 DAOSCV010000000
VERSION     DAOSCV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_11823_cov_3.508923"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1846)
                     /locus_tag="PLY56_00875"
     CDS             complement(110..1846)
                     /locus_tag="PLY56_00875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20062.1"
                     /translation="MIGDLHELPKSWIGEGEVSLPLGKPAELGLSLFLTHPRAFSAVC
                     RHLSAPPALLENDRRVRESFDVVPRQWVKLHYRDGALSGYSLYFHVDPGIQHPVTTLR
                     TFLRRYGHADVSWVEPMLQPALERPETTWGLVLKDAGSRPRPRLSAVIQRGILPDLLE
                     RMVSTGFLTPDQARAYLAWNERAAAEETAYFSFDPGVRGASALDLTRPEASCLPEAWD
                     RLCLDPTGKTLPRYLKCRIRPGSDEPEWSAYLPVAALLDLHATSPNAVTEGILTTFAR
                     NSREPFPSNLEEYREEVRDYYDSQCPAILTALGPTYQAGLVRSKKEEPSAADFQVPEE
                     LRTLAQRQASKAEDARESNRELARRAGIQPGQCILDAGCGAGGPAIDICTAYPGVTVE
                     GITLSPVQAEAGQRLVQEAGLSERVRIIAGDYHALPYEDRSFDVALFLESSGYAYDRD
                     RLFAETFRVLRPGGRLYIKDVFRKEGELSWRERQELQEKRWIYGYRPATLIEVMDAIR
                     NAGFTDLGSASLDEIMSTEHLFEGMLVKRVEIPLLPQFGAPILTDFGRYHFHEFERLP
                     VVFAEVWATKPE"
     gene            complement(1877..2737)
                     /locus_tag="PLY56_00880"
     CDS             complement(1877..2737)
                     /locus_tag="PLY56_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20063.1"
                     /translation="MQLRISSAADLVAALMAPDMGTRMAVLRAIQKDPERALAFGKYE
                     GQDVIDVLIHLGYQEHRYTYWKMLLDTLALYRDSRVTFFFKKLITLAERPEILGVAAR
                     YLSGEPAETVYSHLSALLHGETQEARLRAVATVLASAAAPLLTSEEQVRVGLFREEGA
                     PPPCDEAHIESWLAELEGERADRARALLEAQGEPAFLALKSRWNELSEENREWILRWG
                     ARAHPVDTVDLLTEALRSGDPRRVCTALECVPQLGPAGALFAPMISRLREHPEESIRV
                     AAERAATGEG"
     gene            complement(2802..3953)
                     /locus_tag="PLY56_00885"
     CDS             complement(2802..3953)
                     /locus_tag="PLY56_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20064.1"
                     /translation="MATISRTIIGTDDTGYDLCKGFALFPKLGTLRAGLTVGDRYSPI
                     ISGIRINAVGMYFDLSAGVSFFAGICGPAYAFKFALQGNVDNNLCELTVTEDTTAAGI
                     VWGVRVVFGINIGAYLLKMRWVWDGWNSRLETYYERAFGTQQSIPIDVMDIVIPIIRQ
                     IIKKVCSTDIAIDRANMAATAAGNLWGIYDERRNTFVKNNGSMRVDPRFMVPIDIVSL
                     HPKMKAVMTALATIGGHFRTGPEFGFALPTFIKLNKATVDGAIYGNPTASGSLVRMTK
                     TGGSAAPANPRNITLEMMHEPGFDLRVGWFFSIGVFYVLSFGKDISIPILELLGIRIS
                     LGKYYNSISNTVGATSTGRCIEMGAAGHEGEFEPIEVVFEPAEDPALVG"
     gene            complement(3965..5323)
                     /locus_tag="PLY56_00890"
     CDS             complement(3965..5323)
                     /locus_tag="PLY56_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20065.1"
                     /translation="MANRRDFLRLLGAGLGGTLLSSCGSSSGTAKVGPGGGSGTTPLP
                     NALHFTPVLDVANGRVTRGGRNLDLSDLTPGAVMMNDQNQIIFYGTGSGGNLGIHQLT
                     MDYGRGRAPSVADSQTLLRQGDTVAERSVDLIYPGDTNSSGSFGVIIGSDGQTPALIM
                     RNGPMGAFERILEAMMDLPGGQGFFGGSIDTFSLGANDDILLVARYSQTDQPASAQGA
                     FLMPGASVDNSQILTSSGQPAPQSDGVIDSIGLVDLGEGGAYVLMVNTSPANPARRPA
                     RAPGESRCNTAVIYGHVRQAFSRSRLLTASPVLGASRAASTGESYLAARVGKDDSAMR
                     VVHVTEEHLVMYFRDQVIAETGGLSPSGHVIRSITPGVVNGPAGLCYYQLFTDEGLEL
                     CVSNGVEHRTLLSRGDKIDGKVVNTFIFGFHPEQVDSQGRCVIQVEFTDGTMSIVVAF
                     PA"
     gene            complement(5615..6778)
                     /locus_tag="PLY56_00895"
     CDS             complement(5615..6778)
                     /locus_tag="PLY56_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ20066.1"
                     /translation="MSARQLRIRNAQDLIEGLTSPEVSVRLAVLGATGRNPQAALAYG
                     AHEGRDVIDTLIEQATLAQGTSLWDAFAGVLSAFDDPRAEAFFREVLAEAEEPKRLIL
                     AEAYLSQRDVAPLRDLLSTMLLQSQSAPRARTAARLLTSCGPHSPEESIRMALVLELG
                     EWTPPALDQTTAPHWISELQGPFDREARDLLETQGESAFQLLIGHWDAFSEENKEWLL
                     RWGSRLSSPEVEVLLRRALDSGQDTLMLVALECLAERGGPSEDLAEALHRLVQHPNPA
                     IRLAAIRAGAQGIDWREMLAREADRGIRRECISRLAQEEGARALDDLVQLLENEEWDL
                     RAAATSALIGLGSAVIPAMRRLVEHPAEGVRVAAVRVLVELGDDLWLEEKLIT"
     gene            complement(6775..7569)
                     /locus_tag="PLY56_00900"
     CDS             complement(6775..7569)
                     /locus_tag="PLY56_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ20067.1"
                     /translation="MADLVEHVALTVNGETVSLRDALKRALWRGELGFLQDARDAVLI
                     RQAAEERGITVSDDELQEAAAAFQDEHELYEAAAVEAWLESRSLTLDDWGAHLEQELL
                     ARKLQEALTEGKVEQYFAENILSFESATISQILVDDVDVAEELRAQVVEDGLDFYALA
                     RKYSLDEETRPASGYVGAVTRRSVDAGMSAAIFGAKPGEVVGPVQTERGWHVIKVESR
                     KTPLLDEETAAHIRNLLFQEWLAEQRDKARWSIPLLREIAGEESAA"
     gene            complement(8042..8203)
                     /locus_tag="PLY56_00905"
     CDS             complement(8042..8203)
                     /locus_tag="PLY56_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20068.1"
                     /translation="MAKVQPTDRPKPQKELSPDEAKQVQGGASNAKEMLKRLNKQAGD
                     ILTGDKENK"
     gene            complement(8429..9502)
                     /locus_tag="PLY56_00910"
     CDS             complement(8429..9502)
                     /locus_tag="PLY56_00910"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOJ20069.1"
                     /translation="MQRWRERSRCSWVMGMRRAARLWVPLLLALAPAGSLRADELVFG
                     EQERLKGRVVKMADGQLLLRSEAAGEVRVPWASITDLHTMEPLAVQTRDGRRLVGVLE
                     GMQEGKLRLATDEGMQLVEASLVTWIGPEEETPSAPPAPPKVQWTGNVELGLGLLAGN
                     SRRQDYHFGTEIRRRTRVDRWHLTASAHHGESQGNTDISRAALGLTYSRNLWGRHYYS
                     TGAFLNHDDMKRLRLGGYYFLSKGYRLIDRAPNRWDLEIGGTYVNDNFRYQQRDSFNG
                     LVRSITEVRVFGGSTLKAVWMIWPSLDAIRHVRGTATVSLDVPIARKASLRIGIAEEY
                     DSRPPANTQPHDFQSTMSLVYRL"
     gene            9970..>11823
                     /locus_tag="PLY56_00915"
     CDS             9970..>11823
                     /locus_tag="PLY56_00915"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOJ20070.1"
                     /translation="MSEHAELNQWVIEMARMCQPDKIVWIDGSEEEKERLTQEAFKTG
                     ELIPLNQEKLPGCVYHRTAVNDVARTEDLTYICTRLRDDAGPTNNWMSPEEGYRRAGE
                     IFRGAMRGRTMYVIPFAMGPVGSPFTKYGVELTDSIYVVLNMRIMTRIGKPVLKQLGT
                     AGEFTRCLHSKADLDIERRLILHFPEDNTIWSVGSGYGGNVLLGKKCLALRLASHMGK
                     REGWLAEHMLIMGVEEPNGRIEYVAAAFPSACGKTNLAMLVPPEGLKSKGYRIWTVGD
                     DIAWMRIDTDGRLWAINPERGFFGVAPGTNSRTNPNMMKTIEKNTIYTNVLLGPDGTV
                     WWEDGEGDPPAEGLDWQGRPWKPGMVDENGKPILGAHPNSRFTAPLSQCPVTSFRTEH
                     HHGVPISAIIFGGRRARLAPLVYEAFDWEHGVYVGATMASERTAAQFGKQGEVRRDPM
                     AMLPFCGYHMGDYFRHWLEMGRRMSDPPRIFHVNWFRQDENGHYLWPGYGENLRVIEW
                     ILARCRGEADALETPIGFVPTTDSLDMTGLDIPRETMRKLFAVSRPEWMEEHAQVTEF
                     FEKFGSRLPRELREQHDALARRLEAPISFLKPGTEIRPLAAELNEAIEEQNP"
BASE COUNT         2149 a         3935 c         3708 g         2031 t
ORIGIN      
        1 gcacacgggg atggccgtcg cgggcgcggt tcggtggttg ccctcctcgc cgcgtctcct
       61 tccaggcatc ctcactgccc cagggtcccg ggcatccgca tggccaatct tactcgggct
      121 ttgtcgccca cacctccgcg aagaccacgg gaagacgctc gaactcgtgg aagtggtacc
      181 tcccgaagtc ggtcaggatc ggcgcaccaa actggggaag gagcgggatc tccactcgct
      241 tcaccagcat tccctcaaag aggtgctctg tgctcattat ctcatccaga ctggcgctgc
      301 cgagatcggt gaagccggcg ttacggatcg cgtccattac ctcgatgagc gtggcagggc
      361 ggtagccgta aatccagcgc ttctcctgca actcctggcg ctcgcgccat gagagctcgc
      421 cttccttccg aaagacgtcc ttgatgtaga gccgcccgcc cggccggagg acgcgaaacg
      481 tctccgcgaa aagccggtcg cggtcgtagg catagccact cgactcaagg aacagcgcca
      541 catcaaagga gcgatcctca taagggagcg cgtggtaatc cccagcgatg atccgcaccc
      601 gctcgctcag gccggcttcc tgaaccaggc gctggcccgc ctctgcctgc acgggcgaga
      661 gggtaatccc ctcgaccgtc accccggggt acgcggtgca gatatcgatg gcaggcccac
      721 cggcgccaca accggcgtcc agaatgcact gccccggctg gatgccggca cgtcgcgcca
      781 gttcccggtt ggactcacgc gcatcctccg ccttgctcgc ctgccgctgc gccagggtcc
      841 gtagctcttc gggcacctgg aaatcggccg ctgatggctc ctctttcttg gagcgaacca
      901 gcccggcctg ataggtgggc cccagcgccg tcagaatcgc cgggcactgc gagtcgtagt
      961 agtcgcgcac ctcctcgcga tactcctcga gattcgatgg gaaaggctcg cggctattcc
     1021 tcgcaaacgt ggtgagaatg ccctcggtca ccgcgttcgg tgaggtggca tggagatcga
     1081 gcagcgcggc gacaggaaga taggcggacc actccggctc gtccgaaccg gggcggatcc
     1141 ggcacttcag gtagcgcggg agggtcttcc cggttggatc aaggcagagt cgatcccaag
     1201 cctccgggag gcaggacgcc tccggacggg tcaggtcgag ggcactggcg ccccggaccc
     1261 cgggatcgaa gctaaagtag gctgtctctt cggcggccgc tcgctcgttc cacgccagat
     1321 aggcacgcgc ctgatccggc gtcaggaacc cggtcgagac catgcgctcc aggaggtcgg
     1381 gcaggatgcc tcgttggatc acggcggaga ggcgagggcg cgggcggctc ccagcgtcct
     1441 tgagcaccag gccccaggtc gtctcggggc gttccagcgc tggttggagc atcggctcca
     1501 cccagctcac gtccgcgtga ccataacggc gcaggaaggt gcgcagagtc gtcaccgggt
     1561 gctggatccc cggatccacg tggaagtaga gcgagtagcc tgagagcgcc ccgtcacgat
     1621 agtgaagctt cacccactgc cgcgggacga catcgaagct ctcacgcacg cggcggtcgt
     1681 tctccagaag cgccgggggg gcactcagat gccggcacac ggcggagaag gcgcgcgggt
     1741 gcgtcaggaa gaggctgagg cccagctccg ccggcttccc cagcggaagg ctcacctcgc
     1801 cctccccaat ccacgatttc ggcagctcgt gcagatcccc gatcatcccc tggccattcc
     1861 aatcacgaag ctgagatcat ccctcgccgg tggcggctcg ctcggccgcc acacggatcg
     1921 attcctccgg atgctcgcgg aggcgcgaaa tcatgggcgc gaaaagtgcg cccgccggcc
     1981 ccaattgggg cacgcactcc agcgcggtgc acactcgcct cggatcaccc gagcggagtg
     2041 cttccgtcag aagatcaacc gtatccaccg ggtgggcgcg cgccccccag cggaggatcc
     2101 actcccggtt ttcctcggaa agctcgttcc agcgcgattt gagcgccagg aacgccggct
     2161 cgccttgcgc ttcgagcaag gcacgcgctc gatcggcgcg ctccccctcg agctccgcga
     2221 gccacgattc gatatgcgcc tcatcgcacg gcggcggagc gccctcctcg cggaagaggc
     2281 cgacccgcac ctgttcttca gacgtcagca ggggcgccgc agcgctggca agaacggtgg
     2341 ccaccgcgcg aaggcgtgct tcctgggtct cgccatggag cagcgccgag aggtgcgagt
     2401 acaccgtctc ggctggctcg ccggagagat agcgcgcggc cacccccagg atctccgggc
     2461 gctcggccag ggtgatcagc ttcttgaaga agaaggtcac gcgtgagtcc cggtagagcg
     2521 ccagggtatc gaggagcatc ttccagtagg tgtaacggtg ctcctgatag cccagatgga
     2581 tcaggacgtc gatcacatcc tggccctcgt atttgccaaa ggcaagcgcg cgctcaggat
     2641 ccttctggat ggcccgcagc actgccatgc gcgtgcccat gtccggggcc atcagcgccg
     2701 ccaccagatc tgccgcactg ctgatgcgca actgcatagc gattaccgtg gcgtatccgg
     2761 gagcaggccg cctgcgggcg ccctactccc ggatggacta tctacccgac gagcgcgggg
     2821 tcttccgcgg gctcgaacac cacctcgatc ggctcgaact cgccctcgtg gccggcagcc
     2881 cccatctcga tgcaccgccc ggtggacgtg gcacccacgg tgttggagat ggagttgtag
     2941 tacttgccga gactgatccg aatgccgagg agctcgagga tcgggatgct gatatccttt
     3001 ccgaaggaga gcacgtagaa caccccgatg gagaagaacc acccaacgcg caggtcgaac
     3061 ccgggctcgt gcatcatctc gagagtgata ttccgcgggt tcgcaggagc cgcgctgcct
     3121 ccggtcttgg tcatgcgcac gaggctgccg ctcgcggtcg gattgccata gatggcgcca
     3181 tccaccgtcg ccttgttcag cttgatgaag gtcggcagcg cgaagccgaa ctccggaccg
     3241 gtgcggaagt ggccgccgat ggtggcaagc gccgtcatca ccgccttcat cttcggatgc
     3301 aacgagacga tgtcgatcgg caccatgaac cgcgggtcaa cccgcatcga tccgttattc
     3361 ttgacgaacg tattccggcg ctcgtcgtag atcccccaga ggttgcccgc cgcggtcgcg
     3421 gccatattgg cgcggtcgat ggcgatatcg gtgctgcaga ccttcttgat gatctgccgg
     3481 atgatgggga tcacgatatc catgacatcg atcgggatgc tctgctgcgt gccgaaagcg
     3541 cgctcgtagt aggtctcgag gcgggagttc catccatccc aaacccagcg catcttgagg
     3601 aggtaagcgc cgatgttgat accgaaaacg acgcgcacgc cccagacgat gccggcggcc
     3661 gtggtatctt cggtcaccgt cagctcgcac aggttgttgt cgacattccc ctgcaacgcg
     3721 aacttgaagg cgtaggccgg gccacagatt cctgcgaaga acgagacacc cgccgagaga
     3781 tcgaagtaca tcccgaccgc gttgatgcgg atgccactga taattgggct gtagcggtct
     3841 cccacagtga ggcccgcccg tagggtgccg agcttcggga agagcgcgaa gcccttgcac
     3901 agatcataac cggtgtcgtc ggtcccgatg atcgtcctgc taatcgttgc cattcgttac
     3961 ctcctcacgc cgggaaagcg acgacgatag acatcgtgcc gtcggtaaac tcaacctgga
     4021 tgacgcagcg gccctggcta tccacctgct ccggatggaa gccaaagata aaggtattga
     4081 ccaccttgcc atcgatcttg tcgcctcgcg agagcaacgt ccggtgctcc acaccgttgg
     4141 agacacacag ctccaacccc tcgtcggtga agagctggta gtagcacaga ccggccggac
     4201 cattgaccac gccgggggta atgctcctga tgacgtgccc gctgggcgat aggccacccg
     4261 tctccgcaat cacctgatcg cggaagtaca tcacgaggtg ctcctcggtg acgtggacga
     4321 cgcgcatcgc ggagtcgtcc ttgccgacgc gcgctgcgag atagctctcg cccgtcgacg
     4381 cggcgcgcga agcaccgagg accggcgaag cggtgagcag acgcgaccga gagaacgcct
     4441 ggcgcacgtg gccatagatg accgccgtgt tgcagcggct ctcgccaggc gcgcgcgcag
     4501 gccgccgcgc gggattggcc ggactggtgt tcaccatgag gacgtatgct ccgccctcgc
     4561 ccaggtccac caacccgatg gaatcgatca ccccgtccga ctgcggagcc ggctggccag
     4621 agctggtgag gatctggctg ttgtcgaccg acgcaccagg catcaggaag gcgccctgcg
     4681 cggacgccgg ctggtccgtc tggctatagc gagcaaccag gagaatgtcg tcgttggcgc
     4741 ccaggctgaa ggtatcaatc gagccgccaa agaagccctg gccgcccggc agatccatca
     4801 tcgcttccag gatccgctcg aaggcgccca tggggccatt gcgcatgatg agcgccggcg
     4861 tctggccatc cgagccgatg atgaccccaa agctaccgct ggagttcgta tcgcccggat
     4921 agatcagatc gaccgaccgc tcggcgaccg tatctccctg gcggaggagc gtctggctat
     4981 cggccacgga gggcgcgcgc ccccgcccgt aatccatggt taactggtgg atgccaaggt
     5041 tgccacccga gcccgtgccg tagaagatga tttggttctg gtcgttcatc atcacggcgc
     5101 ctggcgtcag gtcggagaga tccaggttcc ggcctccccg cgtcacccgg ccattggcca
     5161 catccagtac aggcgtgaag tggagggcgt tcggcagcgg cgtggttcca ctgcccccac
     5221 cggggccgac cttcgccgtg ccactcgaac tcccgcagga ggagaggagg gtacctccca
     5281 gtcctgcgcc gagcagcctc agaaaatcac gtcgatttgc catgcgaacc tccgaattct
     5341 cggcccatca cggagcccgc cggccccgaa tgccgctact gcaactacca agctctctct
     5401 tcatatggaa acggtcctga cccatcctct ggaagaaagc cgcgctctct ggtgcccatc
     5461 gctctttcca ccgtcccgcg cgcgggcgag ccaaaagggc gggcgcgatc ctatcctctc
     5521 cttcatttgg atcgccggga agcgtgacgt actctcgcag gatgggccag cccgtgcgtc
     5581 aggacaaggc cccctggcct gggccggaga gtgttcacgt aatcagcttc tcttcgagcc
     5641 acaggtcgtc gccgagctca acgaggacgc gaacggcggc cacgcgcacg ccctccgccg
     5701 ggtgttccac caaccggcgc atcgccggga taacggccga ccccagtcca atcagcgcgc
     5761 tggtcgccgc cgcgcgcagg tcccactcct cgttctccag gagctggacg agatcgtcca
     5821 aggcacgagc gccctcttcc tgggcgagcc gggagatgca ctcgcggcga atgccgcggt
     5881 cggcctctcg ggccagcatc tcgcgccaat cgatcccctg ggcgccggcg cggatcgccg
     5941 ccaggcgaat tgcgggattc ggatgctgaa cgaggcggtg gagggcctcc gcgagatcct
     6001 cggacgggcc cccacgctcc gccaggcact ccagggcgac gagcatcagc gtatcctgtc
     6061 cggagtcgag ggcacggcgc aagagcacct ctacctcggg cgaggagaga cggctgcccc
     6121 agcgcagcaa ccactctttg ttctcctccg agaacgcatc ccaatgcccg atgagaagct
     6181 ggaaggcact ctctccctgg gtttccagca agtcccgagc ttcccggtcg aacgggcctt
     6241 gcagctccga aatccagtga ggagcggtcg tttggtcgag ggcgggcgga gtccactcgc
     6301 ccagttcgag cacgagcgcc atgcgaatgc tctcttcggg agagtgtggc ccgcacgatg
     6361 tgagcaaccg cgcagccgtt cgggcacgcg gcgccgactg gctctgcaac aacatggtgg
     6421 agaggaggtc tcgcaaaggg gcaacatccc tttgcgagag gtaggcttcg gccaggataa
     6481 ggcgcttggg ttcttccgcc tcggccagca cctcccggaa aaacgcctcg gcgcgcgggt
     6541 catcgaaggc gctcagcacg ccggcgaagg catcccacag cgaagtgccc tgcgcgagcg
     6601 ttgcctgctc gatgagcgtg tcgatgacat cgcgcccctc gtgcgcgcca taggcgaggg
     6661 ccgcctgggg attgcgcccg gttgctccca gcactgccag gcgtacggaa acctctggcg
     6721 aggtcaggcc ctcgatcaga tcctgggcat ttcggatgcg gagctgcctg gcactcatgc
     6781 cgcgctctcc tctcctgcga tttcgcggag gagagggatg ctccaacgcg ctttgtctcg
     6841 ctgctcggcc agccactcct ggaagaggag gttacggatg tgcgccgcag tctcctcgtc
     6901 cagcaaaggc gtcttccggc tttccacctt gatcacgtgc catccccgct ctgtctggac
     6961 tggacccacg acctctcctg gcttggcgcc aaagatcgcc gcgctcatcc cggcatccac
     7021 tgaccggcgc gtcacggcac cgacatagcc gcttgcaggt cgcgtttcct catcgagcga
     7081 gtacttgcgc gccagcgcgt agaagtcaag cccatcttct accacctgag cgcgcagttc
     7141 ctccgccacg tccacgtcat ccacgaggat ctgagaaatc gtcgcgctct cgaaggagag
     7201 gatattctcc gcgaaatact gctccacttt tccttcggtg agcgcctctt gcagcttgcg
     7261 cgccagcaac tcctgctcta ggtgcgcgcc ccagtcatcg agggtgagcg aacggctttc
     7321 cagccatgcc tcgaccgccg cagcctcgta caactcgtgc tcatcctgga atgcggcggc
     7381 tgcctcctgc aactcgtcgt cagaaacggt gatcccccgc tcctccgccg cctggcggat
     7441 gagtacagcg tctctggcgt cctgcaagaa cccaagttct ccgcgccaca gcgccctctt
     7501 tagcgcatct cgaagggaca ccgtctcccc attcactgtc aatgcgacgt gctcgacaag
     7561 gtctgccatc tgctcctctt ctacgaaccc ggacgggaat gaagtcgcct atgccctgcc
     7621 acccccagcc gtcgggtagc ttggtcagcg tatccgttca gcgcgggcac cggccatcct
     7681 ccccgctccc gtgagacgac cgttcgagta gcgacagcac ttgaagggga actgacaaaa
     7741 acctcgactt gaaggggaac tggcaaaaaa acctcacttg aaggggaact ggcaaaaacc
     7801 tcgacaggag taccgatggt gaaggcggcc tcctgccgag gtcgtgcgag caatacagcg
     7861 ccgcatggcc aaaactgcag atccctgggg aagacctttc agcgcgacct ctccgctcgc
     7921 caatgcggag aaaaaccgct ccctattaca ggcgatggtg tggcaacgcg gccatcagcc
     7981 gtgccgttta cgtgcgtggc atccccagac ggccaccgaa acggcgtgcg gatcgcgaag
     8041 gctacttgtt ctccttgtcg ccagtcagga tatcgccggc ctgcttgttg aggcgcttca
     8101 gcatctcttt ggcgttgctg gcgccaccct gaacctgctt ggcctcgtcc gggctcagct
     8161 ccttctgcgg cttgggtcgg tcggtcggtt gcactttcgc cattttctcc tcctcaggtc
     8221 aactggtggg cctcatgcca cgacgcttcc gattccgcct ggatccagta cagaatagag
     8281 cgcctgatgg cctgccctct gacggctgcg gactcgcgcc cgcctcactc cgccgtacgt
     8341 ctgaagaccc tttttctatt atcggcggta cactccgtca cttaagggca cgcgagcaaa
     8401 tcccgcgtgc attcctttca cgccaccatc agagacggta gacgagcgac atcgtcgatt
     8461 ggaaatcgtg tggctgcgtg tttgccggcg ggcgcgagtc atactcctcg gcgatcccga
     8521 tccggaggct cgccttccgg gcgattggca cgtcgagcga aaccgtggca gtgcctcgca
     8581 cgtggcgaat cgcatccagg ctgggccaga tcatccacac cgccttcagc gtggagccgc
     8641 caaacacgcg cacctcggtg atcgagcgca ccaggccgtt gaagctgtcg cgctgctggt
     8701 agcggaagtt gtcgttgacg taagttccgc cgatctccag gtcccagcga ttgggagccc
     8761 ggtctatcag gcggtagcct ttcgagagga aatagtagcc tccgaggcgg aggcgcttca
     8821 tgtcatcgtg attgaggaaa gcacccgtcg agtagtagtg ccgcccccac aggttgcggc
     8881 tgtaggtgag gccgagggcc gcgcgcgaga tgtcggtgtt cccctgcgac tccccatgat
     8941 gggcgctcgc agtgaggtgc cagcggtcca cacgcgtacg ccggcggatc tccgtgccga
     9001 agtgataatc ctggcggcgg ctgttgcccg ccagcaaacc gagcccgagc tcgacgttcc
     9061 cggtccactg caccttgggc ggcgccggcg gggcagaggg cgtctcctct tccggaccga
     9121 tccaggtcac cagcgaggct tccacgagct gcatgccctc gtcggtggca agacgcagct
     9181 ttccctcctg catcccctcc aacacgccaa cgagccgacg gccatcgcgc gtttgcaccg
     9241 ccagcggctc catcgtgtgg agatcagtga tggaggccca aggcacccgg acctcgccgg
     9301 cggcctcgga acgcaggaga agctggccgt cggccatctt gaccacgcgg cccttcaggc
     9361 gctcctgctc cccgaacacg agttcatcgg cgcgaagcga gcccgcgggc gccagagcca
     9421 gcaaaagcgg gacccacagc cgcgccgcgc ggcgcatccc catcacccag gagcatcgac
     9481 tccgctcccg ccatcgctgc atacaaccct ccgccagtgc ccgccatgcc accccccggt
     9541 caagctcccg gcgagcgcca ctccgggcca catccacgct ccctcttccc ggcaccggcc
     9601 gcgccgcaaa gcgattcggt ttcgccagca ggcaagcgga accacgccac ctcgcgcacc
     9661 gttcttctta ttggaggcgg ccgcctcatc cccagcgggg ccggcgggtc cgcgcgggtg
     9721 ccgtgaagac ggcgcatgac cggccgtgaa aagagcgaga ctatccgctt tctccctctg
     9781 tagttcgcgg cgctagcgca ggcacctgcc tgagattcag gcgtcgatca attggatctt
     9841 atttgaagga agatgcaggc gggcgttgaa aagagaatgt tggcgtacct gggtacgccc
     9901 gcataaagca gtctgttgat tgttggagta gaggcccccc gtgggggtgg gtacggaaag
     9961 gaacgctgga tgagcgagca cgctgaactg aaccaatggg taattgaaat ggcccggatg
    10021 tgccagcccg ataagattgt ctggattgac ggttctgaag aggagaaaga gcgcctaacc
    10081 caggaagcgt tcaaaaccgg cgagctcatc cctttgaacc aggagaagtt gcccggctgc
    10141 gtctaccacc gcaccgcagt gaacgacgtg gcgcgcacgg aggatctgac ctatatctgt
    10201 acccggctga gggacgacgc gggtccgacc aacaactgga tgtcgccgga agaaggctac
    10261 cggcgcgccg gcgagatctt ccgcggcgcc atgcggggcc ggacgatgta cgtcatcccc
    10321 ttcgcgatgg ggcccgtcgg ctcgcccttc accaaatatg gcgtggagct gaccgacagc
    10381 atctatgtcg tcctgaacat gcggatcatg accaggatcg ggaagccggt gctgaagcaa
    10441 ctcggcacgg caggcgagtt cacgcgctgc ctgcacagca aggcggatct cgacatcgag
    10501 cgccggctca tcctccactt cccggaggac aacacgatct ggagcgtggg ctcgggctat
    10561 gggggcaacg tgctcctcgg caagaagtgc ctggcgctgc gcctggcgag ccacatgggg
    10621 aagcgcgagg gttggcttgc cgagcacatg ctgatcatgg gcgttgagga gccaaacggc
    10681 cggatcgagt acgtggccgc cgccttcccg agcgcctgcg gcaagacgaa tctcgccatg
    10741 cttgtgccgc ccgaggggtt gaagtcgaag ggctaccgca tctggaccgt cggcgacgat
    10801 atcgcctgga tgcgcatcga tacggacggc cggctctggg cgatcaaccc ggagcggggc
    10861 ttcttcgggg tggcgccggg aaccaactcg cgcaccaacc ccaatatgat gaagacgatc
    10921 gagaagaaca cgatctacac caacgtgctt ctgggtcctg acggcacggt gtggtgggaa
    10981 gacggcgagg gagatccccc ggcagagggc ctggactggc agggccggcc gtggaagccc
    11041 ggcatggtcg atgagaacgg caagccgatc ctgggcgccc accccaacag ccgcttcacc
    11101 gcgcctctgt cgcagtgccc ggtcacgtcc ttccgtacgg agcaccatca tggcgtgccg
    11161 atctcggcga tcatcttcgg aggacgccgg gcccgcctgg cgcccctggt gtacgaggcg
    11221 tttgattggg agcacggcgt ctacgtgggc gcgacgatgg cttcggagcg cacggccgct
    11281 cagttcggga agcaaggcga ggtgcgccgc gacccgatgg cgatgcttcc cttctgtggc
    11341 tatcacatgg gtgactactt ccgccactgg ctggagatgg gtcggcgcat gtcggatccg
    11401 ccgcgcatct tccacgtcaa ctggttccgc caggatgaga acggccatta cctctggccc
    11461 ggttatggcg agaacctgcg cgtgatcgag tggatcctgg cgcggtgccg cggcgaggcg
    11521 gatgccctcg agactccgat tggcttcgtg cccaccaccg atagcctcga tatgaccggc
    11581 ctcgatatcc cgcgcgagac gatgaggaag ctcttcgccg tgagccggcc cgaatggatg
    11641 gaagagcacg cgcaggttac cgagttcttc gagaagttcg gctcgcggct gccgcgcgag
    11701 ctgcgcgagc aacacgatgc gctggccaga cggctggagg cgcccatttc gttcttgaag
    11761 ccgggcaccg agatccggcc cctggccgca gagctcaacg aggcgatcga ggagcagaac
    11821 ccg
//
LOCUS       DAOSCV010000017        11720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3682_length_11720_cov_4.097042, whole genome shotgun sequence.
ACCESSION   DAOSCV010000017 DAOSCV010000000
VERSION     DAOSCV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11720
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_11720_cov_4.097042"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLY56_00920"
     CDS             complement(<1..260)
                     /locus_tag="PLY56_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20071.1"
                     /translation="METRREQWGFTLIELLVVIAIIAILAAILFPVFARARENARKST
                     CQSNLKQLGMAQLAYIQDYDEMTPIHRCGDSGHTYLVCTFEA"
     gene            506..919
                     /locus_tag="PLY56_00925"
     CDS             506..919
                     /locus_tag="PLY56_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20072.1"
                     /translation="MVRILFLPILLVSVSIANAAVKPGENILINGSFDSEDQDGLAFW
                     EKSGVNVSSSRTGGPGKSGVLVFSNPEGKTGVRSTCRQHGQRLAHWVNTFLKARWSTR
                     PLEAGRNARLPKEWMMMGRWGTARTERPGSRGTER"
     gene            924..3458
                     /locus_tag="PLY56_00930"
     CDS             924..3458
                     /locus_tag="PLY56_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ20073.1"
                     /translation="MVRILFLPILLVSVSIANAAVKPGENILINGSFDSEDQDGLAFW
                     EKSGVNVSSSRTGGPGKSGVLVFSNPEGKTGVRSTCRQHGQRLAAGETYKMSATIKTR
                     GFKSPHCGIIIHDNGWYKEYGIQSFPENTNGWQHVEQTFELGKSSNDVYGAAIFAINY
                     VGEIQIAQVKLEAVSAGALAKSSPSRILTEQTRPRLVAMQPLLNAIPLSNPKLTFHFF
                     GKTERALGEYDFVSTIDGNKVRKQRLAPKRNTLDLSGLAAGDHRLDVAIVERASQTEV
                     FRISHVITLKEFPKVDTSAHRRLNNLVVEVLGKPLKATAAPQRFEFSTVRDGWVFIKV
                     DAAKADPGLNVRVGTNDTVVTATTPRLEAFRELPRGNHSLTVSGAASGGKIVVRSIAE
                     VFNYPACANSQVPQNGKYDWEFHRRHVFPAVTTLNGGNVPEANRPEVRRMGLKWLANV
                     GTTNPKDAADLVARMSQSPGMTEPYYDGFTADEQFFSSPAIVDYTEAVRLLKNPENRL
                     IYTWIVGKPALPGIHTDFISASLNASKGRGRLLFEIYCHSRPNEQDATEYLNDRLIDT
                     MKRLEEYYPNARAGTGMILGNFNQIPIISLDVNPEVDYKYYLDMQLHIIATHPLFKDL
                     ATAGYWGSYYDDEELYRWSMRLLRHYCVEGNTEMLSKRYGYRYSPGHLSNCDFTDGLK
                     GWTAEPAEAIQTGSFSGYGKGSQGRWGAQAGTGDTFCVFKRGERPATLTQTATGLTPG
                     KVYTLQFVTADYKDMVARKVAPKKHRLRAILGDGAELIPEKAYVFVDQRNSGKKANDG
                     WARINLHHVRFRATAPSVKITFTDDEAEPGTETALNYIMLKPYYED"
     gene            3959..5032
                     /locus_tag="PLY56_00935"
     CDS             3959..5032
                     /locus_tag="PLY56_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20074.1"
                     /translation="MERIRLGVIGCGGMSRVHLATAGGKSPHTSEALRPFAERVEVYA
                     LADVDLARAEAHRQAYGGEYVTDDPERLFSDPNVDAVLITTWHDTHAAYSLRALESGK
                     HVLIEKPMAMTERECDDIVAAVDRTKLKYMVAFRCRFGRGALDVKREIPHPKNVIAHA
                     RVAGIWPETSWAQDPVKGGGQILSQGCHIVDLMFFLAGSEPESVYATGGVFHHSRPDV
                     IDTINASIRFKNGSVGAFLGGDGGTANLMLGHPLPCGCPFWVMAVDQGRSGLTIDHGH
                     DARFESRVPEAEWKPPYDQKIYSQELGQERASGLPEILPTFARCILRDEEPPATAVDG
                     ARTTRFILKCFESARTGRVIRFE"
     gene            5300..5626
                     /locus_tag="PLY56_00940"
     CDS             5300..5626
                     /locus_tag="PLY56_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20075.1"
                     /translation="MDDERWDDLVDMIDERFGIVKNEKTNVPRRDGSPEWHDAVIFEK
                     GGRRMKLERISRPRVLDVKTYYARRRSGNVREEVTYSETERTHTVRLYEWNGDWVEVD
                     LTAISR"
     gene            5721..5951
                     /locus_tag="PLY56_00945"
     CDS             5721..5951
                     /locus_tag="PLY56_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ20076.1"
                     /translation="MGDERAELADQIERAIANKLRMWAIHVEVTDETVVLQGGVRTSQ
                     EYHQAMEVAHELAGKRGVIAEIIVRQEPTTHE"
     gene            complement(6035..7163)
                     /gene="prfB"
                     /locus_tag="PLY56_00950"
     CDS             complement(join(6035..7093,7095..7163))
                     /gene="prfB"
                     /locus_tag="PLY56_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143597621.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOJ20077.1"
                     /translation="MLDELKKEISELTGRLDEMGDRLDFAGKESAIALKEEQASAPDF
                     WNDPEAAQQKMQELNRLKETVEPWYELRSRLGDLRDLVELAQEEGDDSVAESIQQEIL
                     SIRKRFDELEFQALLSGPHDASNAILEINAGAGGTESCDWAAMLLRMYVRWAEHRGYQ
                     TEITDENPGEVAGIKSATVVISGLYAYGYLKSERGVHRLVRISPFDSNKRRHTSFASV
                     NVLPEIADTSEIVIQPDEIEVETYRSSGAGGQNVQKVETAIRIRHKPTGIVVTCQNER
                     SQLKNKDMAMKILRARLYERQEQERLAKLAEIRGEMRAIEWGSQIRSYVFQPYQMVKD
                     LRTDAETSNIQAVMDGEIDLFIEAYLKRCIPAEETAGTQSP"
     gene            complement(7377..8630)
                     /locus_tag="PLY56_00955"
     CDS             complement(7377..8630)
                     /locus_tag="PLY56_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007087280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20078.1"
                     /translation="MTVAIVGAGHRSVAYARYALDHPDRMKVVAIADPWELRRRTVGD
                     QHAIPEEMRFASYEELAARPPVADAVINGTMDQLHYASAMPLLKAGYHMLLEKPIAQR
                     EEEVRELIATAQKYNRTVMICHVLRYAPFYVKVKELLQSGAIGEIISLHTAENVSYHH
                     MAVAYIRGRWRRRESNPILLAKCSHDLDLLAWYLSGVPAVRVSSFGSLKQFRPENAPP
                     GSAKRCLDGCQIEATCPYSARANYITQGLWGAYAWEPIEHLMPATEEQKLESLRTDNP
                     FGRCVWHCDNDVVDHQSVLVEFANGVTASHDLFCATARPSRTLHIIGSKGELEGDMEA
                     GRLIIRRPNPVRGGEYTEEVVEINVKGDGHGGGDTRLIADFVSVLRGESTSLSTTRIE
                     DSLTGHLIAFAADRAMLTHQVVEIA"
     gene            8937..11399
                     /gene="secD"
                     /locus_tag="PLY56_00960"
     CDS             8937..11399
                     /gene="secD"
                     /locus_tag="PLY56_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOJ20079.1"
                     /translation="MKHRHYFLAILALVIASAWVIFGGQYPFKLGLDLKGGMRVILRA
                     EPPAGEKLTPGLLNDAKNVIQRRVDNTGVAEPLVQTKGGNQIIVELPDIPEEKKQEAL
                     DQFQKLAMLRFVAIPDKYEVVHGEPTTFLDKVTRQQVPSSQVLQEGEVIVTGKELLPG
                     KARGNLSVNGQTEVELRFNAEGRKKFADYTMRNVKKYFGIFLDDEPISVPIVEEPIPT
                     GDGVIRGSFSLEEAQDLANLLNAGALPVPLRIEQVTDVGPTLGKESVDKSLVAGLIGL
                     ALVALFMIGFYRLPGIVAVVALLFYAAFSAAIFKLIPVTMTLPGIAGFILSIGMAVDA
                     NILIFERLKEELNAGKTLRAAVDAGFVRAFSSIFDSNMCSVITCAILWNFGTGPIRGF
                     ALTLAIGVIVSMFTAITCTRTMLYMLVPTSLSHKPQWWGLGYTWMHPPEGTVWRIVER
                     WKLYFGISGALILTSIIFMGLMIGKVGSPVLPGIDFTGGSLMQLKYEAQVPTADQVRQ
                     ALAPSGREESDVTISAGEKRVVIRGKHFASDATQAAAEREQIEKALTEATGKFKVEAF
                     DNVGPTMGKDLTSQALKAILVASVLIVLYLWFRFTSLDFGLVAITTLLHDAFVILGFF
                     AVTGYFLHWEVDSLFVTAVLTIIGYSVHDTIVVFDRIRENQRLKARGEPFELVADRSV
                     TQTVVRSINTSLTVVFVLAALVLFGGATIREFNTAMLVGIIAGAYSTIFFASPLLTVA
                     RTYGERRKQASSRRVEEKPMVAAGGAAPRPKPLPKPQPKPIEAAVSSEDVSGPAAPAA
                     GGSVTKVAPKPQRNIKKKKRRF"
     gene            11531..>11720
                     /locus_tag="PLY56_00965"
     CDS             11531..>11720
                     /locus_tag="PLY56_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20080.1"
                     /translation="MIEPRVEDQVPATASAAVGAAPPMDPAVLAGLERLLERVSQYNP
                     QVDRDLVRAAVELAHAAHR"
BASE COUNT         2114 a         3666 c         3812 g         2128 t
ORIGIN      
        1 attgcctcga aggtgcagac caggtaggtg tgccccgaat ccccacagcg atggatgggc
       61 gtcatctcgt cgtagtcctg gatgtaggcc agctgggcca tgccgagctg cttcaggttg
      121 gactgacagg tgctcttgcg cgcgttctct cgcgcccggg caaagaccgg gaagaggatc
      181 gccgccagga tggcgataat cgcgattacc acgagcaact cgatgagggt aaaaccccac
      241 tgttcccgtc gcgtttccat gtgtgttacc tcccaagagt gctgccggca gcattccttg
      301 gactgcttag gcacgcgccg cccagtcgcc ccatgatagc acttgaacgc tcactgggtc
      361 aacaccttcc tcaaggcgcg gtggtccacg cggcccctcg aggcaggaag gaatgcgagg
      421 ctgccgaagg agtggatgat gatgggccgg tggggcactg cccgaacaga gcggcccggt
      481 tcgcgcggaa cggaaaggta gggacatggt gaggatactc tttcttccga tcctgttggt
      541 ctcggtaagc atcgccaacg cggcggtgaa gcccggcgag aacatcctga tcaacggatc
      601 gttcgacagt gaggatcagg atggtctcgc attctgggaa aagagtgggg tcaacgtgag
      661 ctccagccgg accggtgggc ccggcaagtc cggcgtcctg gtgtttagca acccggaagg
      721 caagacggga gtccgctcca cctgccgcca gcacggccag cggctcgcac actgggtcaa
      781 caccttcctc aaggcgcggt ggtccacgcg gcccctcgag gcaggaagga atgcgaggct
      841 gccgaaggag tggatgatga tgggccggtg gggcactgcc cgaacagagc ggcccggttc
      901 gcgcggaacg gaaaggtagg gacatggtga ggatactctt tcttccgatc ctgttggtct
      961 cggtaagcat cgccaacgcg gcggtgaagc ccggcgagaa catcctgatc aacggatcgt
     1021 tcgacagtga ggatcaggat ggtctcgcat tctgggaaaa gagtggggtc aacgtgagct
     1081 ccagccggac cggtgggccc ggcaagtccg gcgtcctggt gtttagcaac ccggaaggca
     1141 agacgggagt ccgctccacc tgccgccagc acggccagcg gctcgcagcc ggcgagacgt
     1201 acaagatgag cgccaccatc aagacgcggg gcttcaagag cccccattgt ggcatcatca
     1261 tccacgataa cggctggtat aaggagtacg gcatccagtc gttccctgag aacaccaacg
     1321 gctggcagca cgtggagcag accttcgagc tagggaagag cagcaacgac gtctacggag
     1381 ccgccatctt cgccatcaac tacgttggtg agatccagat cgcccaggtc aagctggaag
     1441 ccgtcagcgc gggggccctc gccaagagtt cgccgtcgcg gattctcacc gagcagacgc
     1501 ggccccggct cgtggcgatg cagccgctgc tcaacgcgat tcccctttcc aaccccaagc
     1561 tcaccttcca tttcttcggc aagaccgaac gtgccctcgg cgagtatgac ttcgtcagca
     1621 cgattgacgg caacaaggtc cgcaagcagc gtctcgcccc caagcggaat accctggatc
     1681 tgtcgggtct ggcggcgggc gatcaccgtc tggacgtcgc catcgttgag cgtgcgtcgc
     1741 agaccgaagt gttccgcatc tcgcacgtga tcacgctgaa ggaattcccc aaggtggaca
     1801 cctcggcgca ccggcgactg aacaacctcg tggtcgaagt gctgggaaag ccgctgaagg
     1861 caacggctgc cccgcagcgc ttcgagttca gtactgtccg cgatggctgg gtcttcatca
     1921 aggtggacgc cgcgaaggcg gatccggggc tgaacgtccg cgtgggtacg aatgacaccg
     1981 tcgtgacggc cactacccct cgccttgagg cgtttcgaga gctgccgcgc gggaaccaca
     2041 gcctcaccgt ctccggcgcc gcctcgggcg ggaagatcgt ggtgcgctcc atcgcggagg
     2101 tgttcaacta cccggcctgc gccaacagcc aggttccgca gaatgggaag tacgactggg
     2161 agttccaccg ccggcacgtc ttcccggctg tgacgacgct caacggcggc aacgttcctg
     2221 aggcgaaccg cccggaggtg cgccgcatgg ggctcaagtg gctggcgaac gtcggcacaa
     2281 ccaacccgaa ggatgccgct gatctcgtcg cgcggatgtc ccaaagcccg ggtatgaccg
     2341 agccgtatta cgacggcttc accgcagacg agcagttctt ctctagcccg gccatcgtgg
     2401 attacaccga ggccgtgcgg ctcttgaaga acccggagaa ccggctgatc tatacctgga
     2461 tcgtcgggaa gcccgcgctg cctggtatcc ataccgactt catcagcgcg tcgctcaatg
     2521 cctcgaaggg tcgtggccgt cttctcttcg agatctactg ccacagccgc cccaacgagc
     2581 aggatgccac cgagtacctc aacgaccggc tcatcgacac gatgaagcgg ctggaggagt
     2641 actacccgaa cgcccgcgcc ggcacgggga tgatcctggg caacttcaac caaatcccca
     2701 tcatctccct cgatgtgaac ccggaggtgg actacaagta ctacctggat atgcagctcc
     2761 acatcatcgc cacccacccg ctcttcaagg acctggcgac ggcaggctac tgggggagct
     2821 actacgacga cgaggagctg tatcgctggt ccatgcgcct gctgcgccac tactgtgtcg
     2881 agggcaacac ggagatgctc tcgaagcgct acggctatcg ctactccccc gggcacctca
     2941 gcaactgcga cttcacggac gggctcaagg ggtggacggc ggaaccggcg gaggccatcc
     3001 agaccggtag cttttccggc tacgggaagg gcagccaggg gcgctggggt gcccaagctg
     3061 gaacgggcga caccttctgc gtcttcaagc gcggcgagcg tccggccacg ctcacccaga
     3121 cggccaccgg cctgactccg ggcaaggtct acaccctcca gttcgtcacc gccgactaca
     3181 aggatatggt cgcgcgcaag gtggccccca agaaacaccg cctgcgcgct atcctcggcg
     3241 acggcgcgga gctcattccg gagaaggcct acgtcttcgt tgaccagcgc aactccggaa
     3301 agaaggccaa cgacggatgg gctcggatca acctgcacca cgtccggttc cgcgcgacgg
     3361 cgccttccgt caagatcacg ttcacggacg atgaagctga gccgggaacg gagaccgcgc
     3421 tcaactacat catgctcaaa ccgtactacg aggactagaa gtaccccggt gctcacgggc
     3481 acgccaggtg cccggagcac cgaccatcac gaggttcggg gttgcagggg gctctcatcc
     3541 cctgcagccc cgggcccctc tccatgctgc accgaacgcc gcgcaccagg ccccctcgat
     3601 tgagccgcgc cccgactcca aagggatcgc acaacacagg tcgttgcgtc tgtacttctt
     3661 catccgcttc cgcccccatc atccgcggca tcgacggtat cgccgccggc gcgctcggcg
     3721 tggccaatct cctgttagcg tcggtgggag cttctgtgcc cagcgcggga acgtccggca
     3781 gctgggtcac gcgcgcgcgg tttactgggc gtcctggcct catttcgact gcgtctggct
     3841 gccgtgcggc actctctcgg gcactttcga tccgggcgtg caggaaacgc tctggccagg
     3901 gcgaatcgga cgggaaggct ggcccggtgc catgcgccgg cgcccggagg agcaggagat
     3961 ggagagaatc aggcttgggg ttatcgggtg tgggggcatg agccgcgttc acctcgcgac
     4021 ggcagggggc aagtcaccgc atacgtccga ggcgttgcgg cccttcgcgg aacgcgtgga
     4081 ggtatacgcg ctggcggatg tcgacctggc gcgtgccgag gcgcaccgcc aggcctatgg
     4141 tggcgagtat gtgacggatg atccggagcg cctctttagc gacccgaacg tggatgccgt
     4201 tctgatcacc acctggcatg acacgcacgc ggcatactct ctgcgcgcgc tggagtcggg
     4261 aaagcacgtc ctcatcgaga agccgatggc gatgacggag cgggagtgtg acgatatcgt
     4321 ggccgccgta gatcggacga aactgaagta catggtggcc ttccgctgcc gcttcgggcg
     4381 gggcgccctc gatgtgaagc gagagatccc gcatccgaag aacgtgatcg cccacgcccg
     4441 tgtcgcgggg atctggccag agacgagctg ggcgcaggac ccggtcaaag gcggcggcca
     4501 gatcctctct caggggtgcc atatcgtcga tctgatgttt ttcctggctg gctccgagcc
     4561 ggaatccgtg tatgccacgg ggggtgtgtt tcaccactcc cgcccggatg tgatcgatac
     4621 gatcaacgcc tcgatccgct tcaagaacgg gagcgtgggc gctttcctcg gtggcgacgg
     4681 cggcaccgcc aacctgatgc tgggccaccc cctcccttgc ggttgcccct tctgggtgat
     4741 ggccgtcgat caggggcgca gcgggctcac gatcgaccac gggcatgacg cgcgcttcga
     4801 gtcgcgcgtg cccgaggcgg agtggaagcc tccctatgac cagaagatct actcgcagga
     4861 gctggggcag gagcgtgcca gcggcctgcc ggagatcctc cccacgttcg cacgctgcat
     4921 cctgcgcgat gaggagcctc ccgccacggc ggtcgatggg gcgcgcacga cgcgcttcat
     4981 cctgaagtgc ttcgagtcgg cccgcacggg gagagtgatc cggtttgagt gaaccacgcc
     5041 tctctcggaa gctcacatct cttggccggg cttccgacga gagggccacg cgggtgaacg
     5101 cttcgatccc gaaggggtcg catcacgcca tttgggggta tgcgaccccg ttcgggggcg
     5161 ccgctaccgc cacgcggtgg cttccctggg ccggcgtgga ccaggggcag ggcgctggat
     5221 cgtcgtttga ctccgctcct gcccggtgct ataatggctc cgaaaagggt agcagtcaag
     5281 attagtgggg aggcatggga tggatgacga gcgttgggac gatctcgtcg atatgataga
     5341 cgagcgtttt ggcattgtga agaacgagaa gacgaacgtc ccccgaaggg atggctcgcc
     5401 cgaatggcat gatgccgtca tcttcgagaa gggcggacgg cgcatgaagc tggagcgcat
     5461 cagccggccg cgcgtgttgg atgtgaagac ctattatgcc cgccgccgtt ctggcaatgt
     5521 gcgcgaagag gtcacctact cggagaccga gcgcacgcac accgttcgcc tctatgagtg
     5581 gaacggcgat tgggtcgagg tggatctgac ggcgatctcg cgctgaacat cgcctggctt
     5641 gaggtcggca gcgagggggt agactgcccg tgccggaggc ggatcgcgga gcggaacgtc
     5701 ctggcggagg ggagtcggaa atgggtgacg agagagccga gcttgccgac cagatcgagc
     5761 gcgccattgc caacaaattg cggatgtggg cgatccacgt cgaggtgacg gatgagacgg
     5821 ttgtgctcca gggcggcgtg cgcacctctc aggagtacca ccaggcgatg gaggttgccc
     5881 acgaactggc gggcaagcgc ggggtaatcg ctgagatcat cgttcgccag gagccaacca
     5941 cgcacgaata gcccgttctt ctgcgaaagg cgtgttctgg taaagattcc ggcccgagac
     6001 ggcgtgtctc gggccggtcg ctataggcgg gcggctacgg gctctgggtg cctgctgtct
     6061 cttccgcggg aatacaccgc ttcaagtacg cctcgataaa caggtcgatc tcgccatcca
     6121 tgacggcctg gatgttgctc gtctctgcat cggtgcggag atctttgacc atctgatagg
     6181 gctggaacac gtaggagcga atctggctgc cccactcaat ggcacgcatc tccccgcgta
     6241 tctccgccag cttggccagg cgctcctgct cctggcgctc gtagaggcgc gcccgcagga
     6301 tcttcatcgc catgtccttg ttcttgagct ggctccgctc gttctggcac gtgaccacaa
     6361 tcccggtggg cttgtggcgg atgcggattg ccgtctccac cttctgcacg ttctgaccgc
     6421 cggcgccgct ggagcggtag gtctccacct cgatctcatc gggttggatg acgatctcgc
     6481 tggtatcagc gatctccggg agaacattca ccgaggcgaa tgaggtgtgc cggcgcttat
     6541 tggaatcgaa aggcgagatg cgcaccaggc ggtggacccc gcgctcgctc ttcaagtagc
     6601 cgtaggcata aagaccggag atgaccaccg tggcgctctt gatcccggcc acctcgcccg
     6661 ggttctcatc ggtgatctcg gtctgatagc cgcggtgctc cgcccagcgc acgtacatgc
     6721 gcaggagcat cgccgcccaa tcgcacgatt ccgtgcctcc cgcgccagcg ttgatctcca
     6781 agatcgcgtt gctggcatcg tgcgggccac tcagcagcgc ctggaactcc agctcgtcga
     6841 accgcttccg gatggagagg atctcctgct ggatgctctc ggcaacgctg tcatcgccct
     6901 cctcctgagc cagctccacg aggtcgcgca agtcgcccaa tcgcgagcgc agctcgtacc
     6961 agggctccac agtctccttc aggcggttca gctcctgcat cttctgctgt gccgcctccg
     7021 ggtcgttcca gaaatcaggg gccgatgcct gctcctcttt cagtgcaatc gccgactctt
     7081 tgccggcgaa gtcaaaggcg atcccccatt tcgtcgagcc gcccggtgag ctcgctaatc
     7141 tctttcttca gctcgtccag caactagact cacccccttt tgctgcccat ccggtggatg
     7201 ggcgcgctgc ctgcccggcc gggcaggtat cgaccaattg catgaccaga acacgaaccc
     7261 ggacggcgat gccgcccggg tcgtgatgtg ggcacacctg tatgataccc tattgtaccc
     7321 catctgcggc gctggcgcaa gtgccgtgcc gggtggggcc tgcggcgagg cgctctctac
     7381 gcgatctcca ccacctggtg ggtgagcatc gcgcggtccg ccgcgaaggc gatgaggtgt
     7441 ccggtgagcg agtcctcgat gcgggtcgtg ctcagcgagg tcgactcccc gcgcaggacg
     7501 ctgacaaagt cggcgatcag gcgcgtatcc ccgccgccgt ggccatcgcc cttgacattg
     7561 atctcgacga cctcctcggt gtactcgccg ccgcgcaccg ggttgggccg gcggatgatc
     7621 agccggcctg cctccatatc gccctccagc tcgcccttcg agccgatgat atgcagcgtt
     7681 cggcttggcc ttgcggtggc gcagaagaga tcgtgcgacg ccgtcacccc gttcgcgaac
     7741 tccaccagaa ccgattggtg gtccaccacg tcgttatcgc aatgccacac gcagcgtccg
     7801 aacgggttgt cggttctcag cgactccagc ttctgctcct cggttgctgg catcaggtgc
     7861 tcgatgggct cccaggcata ggccccccac agcccctgcg tgatgtagtt ggcgcgggcg
     7921 gagtaagggc aagtcgcctc aatctggcac ccatccaggc agcgcttggc cgatcctggg
     7981 ggcgcgttct ccgggcggaa ctgcttcagc gacccgaagc tcgaaacgcg cacggcgggc
     8041 acgccggaaa gataccaggc gagcaggtcc aggtcgtggc tgcacttggc gaggagtatt
     8101 gggttggact cgcgccggcg ccagcgcccg cggatgtagg ccacggccat atggtgatag
     8161 ctgacattct ccgccgtgtg cagcgagatg atttccccga tggcgccgct ctggagcagc
     8221 tccttcacct tcacatagaa gggggcgtag cggaggacat ggcagatcat caccgtgcgg
     8281 ttgtatttct gcgcggtcgc gatcagctcg cgcacctcct cctcgcgctg ggcaatcggc
     8341 ttctccagga gcatgtggta gcccgccttc aagaggggca tcgccgaggc gtagtggagt
     8401 tggtccatcg tgccgttaat cacggcatcg gcgacaggag ggcgcgcggc cagctcctcg
     8461 taggaggcaa agcgcatctc ttccgggatg gcgtgctggt cgccgaccgt gcgccggcgc
     8521 agctcccagg gatccgcgat ggccaccact ttcatccgat ccgggtggtc cagggcgtag
     8581 cgcgcatacg ccacggagcg gtggcccgct cccacaatcg caactgtcac gggtcgatct
     8641 atcacgtttg cagcctttca gtacggtact acctcaatac aggccattac aggcccgggg
     8701 tgcctgaagg gatccgggac gaaaacgtcg ccgatccacc ccttcccgat agggacgttt
     8761 cacgctcgtg ggcctccttc ctgccgggtt ctgcccgcgc cgagagggcg gatctaggcc
     8821 cttgcggcgg cgggatttgc cttgacaatg gggggagccg tcagtataat gtctaggacc
     8881 gatatcctgc ccttgtgtag tttttgtcgt ggcggggagg cggaaggaga cctcttttga
     8941 agcataggca ctattttctc gccattttgg cccttgtgat cgcttcggcg tgggtgatat
     9001 ttggcggcca gtaccccttc aagctgggtc tggatctgaa gggtggcatg cgggtcattc
     9061 tccgtgctga gccgcccgcc ggggagaagt tgacccccgg attgctaaat gatgccaaga
     9121 acgtcatcca gcgacgcgtc gacaacacgg gtgtcgcaga gccgctggtg cagaccaagg
     9181 gcggcaacca gatcattgtt gagctcccgg atattccgga agagaagaag caagaggcgc
     9241 tggaccagtt ccagaagctg gccatgctgc gctttgtcgc tattccggat aagtatgaag
     9301 tcgttcacgg cgagccgacc accttcctgg ataaggtgac ccgccagcag gtgccctcct
     9361 ctcaggtgct tcaggagggt gaggtcatcg tcacggggaa ggagttgctc ccgggcaagg
     9421 cgcgcggcaa cctgagcgtg aacggccaga ccgaagtaga gttgcgcttc aacgccgagg
     9481 ggcgaaagaa gttcgccgac tacacgatgc gcaacgtgaa gaagtacttc ggcatcttcc
     9541 tggacgacga gccgatctcc gtgccgatcg tcgaagagcc gatcccgacg ggcgacggcg
     9601 tcatccgcgg cagcttctcc ctggaagagg cgcaggacct ggccaacctg ttgaacgcgg
     9661 gcgccctccc ggtgccgctg cgcatcgagc aggtgaccga tgtcggcccc acgctgggta
     9721 aggagagcgt cgataagtcg ctcgtggctg gcttgatcgg cctggcgttg gtcgccctct
     9781 tcatgatcgg cttctaccgc ctgccaggca tcgtggcggt ggtggcactc ctcttctacg
     9841 cggcgttctc ggcagcgatc ttcaagctga tcccggtgac gatgacgctg ccaggcatcg
     9901 cgggcttcat cctgtcgatc ggtatggcgg ttgatgccaa catcctgatc ttcgagcgcc
     9961 tgaaggagga gctcaacgcc ggcaagacgt tgcgcgcggc ggtggacgcg ggcttcgtgc
    10021 gagcgttctc gtcgatcttc gactcgaaca tgtgctcggt gatcacctgt gccatcctct
    10081 ggaacttcgg cacgggcccg atccgtggct tcgccctgac gctggcgatc ggtgtcatcg
    10141 tgagcatgtt caccgccatc acgtgcacgc gcaccatgct ctacatgctg gtgccgacca
    10201 gcctgagcca caagccgcag tggtggggcc ttggctacac gtggatgcat ccccccgagg
    10261 gcaccgtctg gcggatcgtg gagcgctgga agctctactt cgggatcagt ggggcactga
    10321 tcttgacgag catcatcttc atgggcttga tgatcggcaa ggtcggctcc ccggttctcc
    10381 ctgggatcga cttcaccggc ggcagcttga tgcagctcaa gtatgaggcg caagtcccga
    10441 ctgccgatca ggtgcgccag gcgctcgccc cgtccgggcg cgaggaatcg gatgtcacga
    10501 tcagtgccgg ggagaagcgc gtcgtgatcc gtggtaagca tttcgcctcg gatgcgactc
    10561 aggccgcggc agagcgtgag cagatcgaga aggcgctgac cgaagcgacc ggcaagttca
    10621 aggtagaggc gttcgacaac gtgggcccga cgatgggtaa ggatctgacg agccaggcgc
    10681 tcaaggcgat cctggtcgcc tcggtcctga tcgtcctcta cctgtggttc cggtttacga
    10741 gcctggactt cgggctggtg gcgatcacca ccctgctcca tgatgccttc gtcattctgg
    10801 gcttcttcgc cgtcacgggc tacttcctcc actgggaagt ggatagcctc ttcgtgaccg
    10861 cggtgctgac gattatcggt tactcggtcc acgacaccat tgttgtcttc gaccgcatcc
    10921 gagagaatca gcgcctgaag gcgcgcggcg agccgttcga actggtggcc gaccggagcg
    10981 tgacgcagac ggtggtccgc tcgatcaaca cctcgttgac ggtggtcttc gtgctggctg
    11041 ccctggtcct ctttggtggg gccacgatcc gagagttcaa tacggccatg ctcgtaggca
    11101 tcatcgcggg agcctactcg acgatcttct tcgcctcgcc gctgctgacg gtggcgcgca
    11161 cttacggcga acggagaaag caggcgagct ctcgccgggt ggaagagaag ccgatggttg
    11221 ccgccggtgg tgcggcaccg cggcccaagc cactgccgaa gccgcagccc aagccaatcg
    11281 aggccgcggt ctcgagcgag gatgtctccg ggcccgccgc accggcggct ggcgggagcg
    11341 tgaccaaggt ggccccgaag ccacagcgga acattaagaa gaagaaacgc cgcttctaac
    11401 cagggagcga gcggttggtg tggaagagcc tgcggcctgg aacggcccca ggctcttcct
    11461 gtacctagcc gaagtgaggt gcgagcaata gatgggcgcg gatgcgccaa gatatggaaa
    11521 catgacccag gtgattgagc cgagagtaga ggaccaggta ccggccacgg catcagcagc
    11581 ggtgggggcc gcccccccga tggacccggc ggtcctcgcc ggcctggaac ggctgctgga
    11641 gagggtgagc cagtacaacc cgcaggtcga ccgcgacctg gtgcgcgcgg cggtggagct
    11701 ggcgcatgcg gcgcatcggg
//
LOCUS       DAOSCV010000018        11087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3925_length_11087_cov_3.314993, whole genome shotgun sequence.
ACCESSION   DAOSCV010000018 DAOSCV010000000
VERSION     DAOSCV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3925_length_11087_cov_3.314993"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PLY56_00970"
     CDS             <1..1317
                     /locus_tag="PLY56_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOJ20081.1"
                     /translation="TDHVGVSRKIDEEAERDRLKQLAHQVKPQGCGLIVRTEAEGRGL
                     DELKNDAEFLVKLWQTIQERAKSATAPAVIHTELSLLFRTVRDSFVSDVSKFIVDSPS
                     AYEKILELVEMLSPRLKSRVELYRKRDPIFHHFGIDQEIERLLRPRIWLKSGGYLVID
                     ESEALTTIDVNTGKFIGSTSLADTILRTNLEAASEVARQLRLRDIGGIIIIDFIDMDT
                     KKDKDAVMAALKNELTRDRARTKVAHISPLGLVEMTRKRTGETIIDQMTSHCPYCAGR
                     GKILSPETVSMRCERDLGKMAAEVDDEAFMITMHPDVAYMFIGAGGDNIQRLEETIHR
                     AIYVRSDDSIHREHYRIVPGDMQEMDRQLQRIKPRQIVELPIVKHPLTQAPRAAAWAD
                     GYLIDLENAAPFIGERAKVRVRKMNRSFAIGEVVLPAKVVDKSEPI"
     gene            1456..2808
                     /locus_tag="PLY56_00975"
     CDS             1456..2808
                     /locus_tag="PLY56_00975"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOJ20082.1"
                     /translation="MPVPVSLNPEYLAPEVTVDEILAEQANVRLWHDKLHDGSGPGAE
                     FRGWLEPDQIVSPDELDRLIATANELRETCDCLVVVGIGGSYLGARAAIEALASPSAP
                     PVLYAGQNLSASYHARLLEALKGKEFALNVISKSGTTTEPAVAFRILREHLEARVGKE
                     AAAKRIVATTDARKGALRALATQQGYRTFVVPDDVGGRFSVLSAVGLLPIAFAGIDVR
                     AMVQGAAACAEACRCTDLMRNPAYLYAVARNRLYRKGFAVEILSSFEPRLHTLAEWWK
                     QLYGESEGKQQMGLFPASADFTTDLHSLGQYIQQGRRILIETFLEIAGGEPEVRVPSD
                     AQDGDGLNFLAGKTLAEINREAYRATALAHREGGVPNQTITLERLDAFSLGALFYFFE
                     KACALSGYLLGVNPFDQPGVEAYKKHMFALLGKPGFEAQTRELRERLETGAASPEIRF
                     "
     gene            complement(2912..5476)
                     /locus_tag="PLY56_00980"
     CDS             complement(2912..5476)
                     /locus_tag="PLY56_00980"
                     /inference="COORDINATES: protein motif:HMM:NF036826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20083.1"
                     /translation="MWCFSLILLLVTAGAASAQGWEENFDGPALDPRWTWRVPVEGPT
                     YSLTERPGWLRVRIPQKENGFNHWNEPGQIDEAPQLRTRVPEGDWELEARVQLQEFDP
                     GSNFHAGLMVGTSDAELLAWGIFYGPGLPGGQQAPEAWLEVTGKSGYMKVGGEARDLV
                     LRLTKVGRTYLAGLRRGEGDWVDGGTYTLLRPPQFLGIIGKTFLNGPGITFDVDYIRL
                     IPRAERELPPLEATVRVDGRAERPALSPFRASYTLECLGRSAGGGPWAEMLANRKFAG
                     PAGETGVVDGWTPVGAGAGVTFAPDNRIFYAPVQSQRITRAQAGEACGIAQSGLVLRE
                     GIGLMGRLVAREEGEIGPVSITLLAGEEVLAERQIRLPDARWHTEEFTFPKPGRDVAG
                     ATLRITFEGAGTLWIGAVSLMPADSEGGWRKDVIEAARALHPAMICWPGSRLAAGYHW
                     EDGIGPRDQRPARWNRAGGAWETNDVGTHEFLEFCRRVGAEPCLVVNAGESAAREAAR
                     WVEYCNGLAESAQGRIRAANGRTEPFGVRTWTLAGNGTGPGGLSLAHGFHDAVRAVEF
                     AQAMRGVDSNIRVAGLSVESRGRDAWSRSQLAAAAPELDYLSVRYSRPLEPADDPLMA
                     YSAAVAGAAQLEAQLQRLAATASASAPAERPVRLALDEWSLTVEKPGAAPEQSFAAGL
                     FACGVLNALTRLGDRVDRAHLGLWTPSGGALQVTPARVDATPVYLALKLHAGRSGVSG
                     VPVAVEGGRVTLPGVGEAPAIDAAASLSKDGRELYLSLVNRHPFQAATVKIEPQGLRL
                     REGGEWAALVSEEIDSQNAPDKPDAVRIETRPLKPGEHREITLPAHSAAVLTYRVR"
     gene            complement(5767..6756)
                     /locus_tag="PLY56_00985"
     CDS             complement(5767..6756)
                     /locus_tag="PLY56_00985"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003521247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOJ20084.1"
                     /translation="MMKVKGKTAMAQMQAMEPISKVVIVGAGRVGSTTAYTMMTRGTA
                     AEIVLIDADHERAIGEAMDISHGLPFGGTVRVEAGDYSDCAGADIVIISAGVAQKPGE
                     TRIDLVRNNIEVLKEIIPKITAHNQDCILMMVTNPVDILTYAMWKISGFPPNRCLGSG
                     TSLDTARFRYSLGKYFEVAPRSVHAYIIGEHGDTEVPVWSLANIAGMRLTDFCRATKL
                     CHDDRDLRVIFENVRDAAYHIIQRKGATHYAIGAAVDAIVGMILHDQHTVVTVSSLME
                     GQYGVHDICLSLPTVIGRAGVVRVLELKLSDAEVEGFRKSAEALKEVARSVGL"
     gene            complement(7005..9314)
                     /locus_tag="PLY56_00990"
     CDS             complement(7005..9314)
                     /locus_tag="PLY56_00990"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ20085.1"
                     /translation="MGASAILSGARVWARRQPVAAAALFLLFLLFLQFPGVLLLRQVP
                     FVSEMVAGSDLLDFNYPVRRILGDALRRGTLPHWTDAMNTGFPIHGEGQGGFLYPPNL
                     LLGWLPLVASINLSLVLTYLGAGLLMFLYARELEQSRVAALFAGMVFAFSGFFFGHIR
                     HLNLIAAAQWLPLLLLLCERYARTRRPAYLLALAPVQALQHLAGHPQTAYYSILFTGV
                     YLILRVLQREVSVDATPSSPGRLALRSLRAAALAAAGFVLAAGLGAGLAGVQLLPTWE
                     LAQLAHRSAKDSLEKATYYTLPWQHLVTFVSPFFFGDPGRNTYSFFAGGQAVLFWENC
                     GYVGLLPLVLALYATGALWRRDRRVALLAGAAVVSLLLAMGKQTPLFPFLWRHLPGMG
                     EFRIPARFLLVTDLALAALAGYGFDLARSRLATWRLRLAPALVGLCFALTAAELLSHA
                     SRQYALVDARKWLGPAPVMAAIGADRDQCRVYTWHEVVPWQDALEHSRGWQRDPEAFY
                     PLRNTLLPNFGVLQGMPHYSAYTGFFFTDKGRLDAQINRLLAGQSPDAIRLGLRLLGL
                     YNAGYITAPDDLSPGGARLIQGFPAAGRREASVRLWRNPYLLPRAYLAPTAEAVDGLR
                     GFLERFARPDHDPRQTVLLDAREAVEGASARATGRAPGQVRWVSSEAERIVLEVESPG
                     GGWLVLSDYNYPGWRARVDGELTPIYRANVIARAVRVPPGRHEVVFTYEPLSWEQGIL
                     VSSLSLAVLLGLIGLCLRRRRYHAMKGTS"
     gene            9524..10429
                     /gene="murQ"
                     /locus_tag="PLY56_00995"
     CDS             9524..10429
                     /gene="murQ"
                     /locus_tag="PLY56_00995"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOJ20086.1"
                     /translation="MRRFEELATEGINPEHARLDELPTLELVTAMNQEDRSVADAVAA
                     VLPEVARAVDLVVSALGNGGRLFYVGAGTSGRLGALDAAECPPTFSVSRNVVQAIIAG
                     GPEALLHSVEAVEDSEEGGAEQITLASVSNLDIVCGLSACGSTPFVLGAMRAARERGA
                     RTIGIACNRPSEMEPLADVMITPLTGPEILSGSTRLKAGTAQKMILNMLSTAAMVRLG
                     KVYGNLMVDVHPVSKKLADRAQRIIRVATGVSEEEARALFEESGGRPKVAIVMQKRHC
                     TPKRAEELLARNKGFVKAALADQRP"
     gene            10426..>11087
                     /locus_tag="PLY56_01000"
     CDS             10426..>11087
                     /locus_tag="PLY56_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20087.1"
                     /translation="MNAWPRMAGGAPRLSRRVLTGILLGLAVLAVPAAPTPTDGPVIR
                     VGLGQRATAVRLSSRAGLQVQGPDGGLLLEVRRAVTLQPVTLQPVAPHAGTRQPVARE
                     AGARQPGASNADARQPGAPDAATPESPAHGTPETAPPETNEAPIVPDRPAGPLLSATD
                     TSDGEAPALAIPFSTLRIVPCEEGAPLDLGKRRYRGTIEVRVDARGRLTVERRSRRAN
                     GKA"
BASE COUNT         1902 a         3818 c         3713 g         1654 t
ORIGIN      
        1 accgaccacg tcggcgtgtc gcgcaagatc gatgaagaag ccgagcgcga ccgtctcaag
       61 cagcttgctc accaggtgaa gccgcagggg tgtggcctca tcgtgcggac tgaggcagaa
      121 ggccgcggtc tggacgagct gaagaacgat gcagagttcc tggtgaagct gtggcagacg
      181 attcaggagc gggccaagag cgccactgct ccggcggtga tccacaccga gctcagcctc
      241 ctcttccgca cagtgcgcga ctcgttcgtc tccgatgtga gcaagttcat cgtcgactcg
      301 cccagcgcgt acgagaagat cctcgagctc gtcgagatgc tctcgccgcg gctcaaaagc
      361 cgcgtcgagc tctatcgcaa gcgcgacccc attttccatc atttcggcat cgaccaggag
      421 atcgagcgcc tgcttcgccc gcgcatctgg ctgaagtcgg gcggctacct ggtgatcgat
      481 gagagcgagg cgctcaccac catcgacgtg aacaccggaa agttcatcgg cagcaccagc
      541 ctcgcagaca caatcctgcg caccaacctg gaggcggcaa gcgaagtcgc acgccagctg
      601 cgtttgcgtg acatcggcgg catcatcatc atcgacttta tcgacatgga cacgaagaag
      661 gataaagatg ccgtgatggc cgcgctcaag aacgagctca cccgcgaccg cgcgcgcacc
      721 aaagtggcgc acatcagccc gctcggcctg gttgagatga cccgcaagcg caccggggag
      781 acgatcatcg atcagatgac ctcgcactgc ccctactgtg ccggccgcgg gaagatcctc
      841 tcgccagaga ccgtgagcat gcgctgtgag cgcgatctgg gcaagatggc agctgaagtc
      901 gatgacgagg cgttcatgat cacgatgcac cccgatgttg cctatatgtt catcggggcc
      961 ggcggcgaca acatccagcg cctggaggag acgatccacc gcgccatcta tgtgcgctcg
     1021 gatgattcga tccaccgcga gcactaccgc atcgtgccgg gcgatatgca ggagatggac
     1081 cgccagctgc aacgcatcaa gccccgccaa attgtggaat tgccgatcgt gaagcacccg
     1141 ctcacccagg caccgcgcgc ggccgcgtgg gcagacggtt acctcatcga cctggagaac
     1201 gcggctccct tcatcgggga gcgcgccaag gtacgcgtcc gcaagatgaa ccgctccttc
     1261 gccattggcg aagtcgtcct ccccgcgaag gtggtggata agtcggaacc gatctaaacg
     1321 gaatcgcatt tgaggtgtcg atacgctcgg gtgggggctc ccccatccgg gcgttccggc
     1381 aggcgaaacg cccgccggaa tggatcttct accggggtgg gggtctcccc cgccccggtc
     1441 tctggaggga taaccatgcc cgttccggtt tccctgaacc cggagtatct tgctccggag
     1501 gtgaccgttg acgagatcct cgccgagcag gcgaacgtcc gcctctggca cgacaagctg
     1561 catgacggca gcgggccggg cgcggagttc cgcggctggc tcgagcccga ccagatcgtc
     1621 tcgccggacg agctagaccg cctgatcgcc acggcgaatg agctgcgcga gacgtgcgac
     1681 tgcctggtcg tggtcggcat tggcggctcg tatctgggcg ctcgcgccgc tatcgaggcg
     1741 ctggcttccc cttcggcgcc ccccgtgctg tatgctggtc agaatctgtc ggccagttat
     1801 catgcacgcc tgctggaggc gctgaagggc aaagagttcg cgctgaacgt catctccaag
     1861 tcgggaacaa ccaccgaacc ggctgttgcc ttccggattc tgcgcgagca cctcgaagcg
     1921 cgcgtgggca aggaggccgc cgccaagcga attgtggcga ccaccgacgc gcgcaagggc
     1981 gcactccgcg cgctggcaac ccagcaaggc taccgcacct tcgtggtgcc cgatgatgtc
     2041 ggtgggcggt tctcggtcct ctccgcggtc ggccttctcc cgatcgcctt cgcgggcatc
     2101 gacgtgcgcg cgatggtgca gggcgcggcg gcatgcgcgg aggcatgccg gtgcaccgat
     2161 ttgatgcgca atcccgccta tctctacgcc gtcgcacgca accggctcta tcggaagggc
     2221 tttgccgtag agattctctc gtccttcgag ccgcgcctcc ataccctcgc ggagtggtgg
     2281 aagcagctgt atggcgagag cgagggcaag cagcagatgg gcctcttccc cgcctcggca
     2341 gactttacca ccgacctcca ctcactgggc cagtatatcc agcaggggcg ccggatcctg
     2401 atcgagacgt tcctggagat cgcagggggc gagccggagg tgcgcgtgcc gagcgacgcg
     2461 caggatggcg atgggctcaa cttcctcgct gggaagacgc tggcggagat caacagggag
     2521 gcctatcgcg cgacggcact tgcccaccgc gaaggcggcg tgcccaacca gacgatcacg
     2581 ctggagcggc tggatgcatt ctcgctgggc gcgctcttct acttcttcga gaaggcgtgc
     2641 gcgctgagcg gctacctcct cggcgtcaat ccctttgatc agccgggcgt ggaggcgtac
     2701 aagaagcaca tgttcgcgct cctcggcaag cccggtttcg aggcgcagac gcgcgagcta
     2761 cgcgagcgcc tggagaccgg cgccgcctcg cccgagatcc gattctaata acccgcgcgg
     2821 gcagccctcc cgctgttccg gtgggagggc cgccccataa ccttccatcc ctggcaacgc
     2881 gatagccaaa gaccctctcg acgcgccggg gtcaacgcac gcgatatgtg aggacggccg
     2941 cggaatgcgc cggcagcgtg atctcccggt gctctcctgg cttcagcggg cgcgtttcga
     3001 tgcgcacggc gtccggcttg tctggcgcgt tctggctgtc gatctcctcg ctcacgagcg
     3061 cggcccactc gccaccttcc ctcagccgca acccctgcgg ctcgatcttc acggtggccg
     3121 cctggaacgg gtggcgattg accagcgaga ggtagagctc ccgcccatcc ttgctgaggg
     3181 acgcggccgc gtcgatcgcg ggcgcctcgc ccacgccggg cagcgtcacc cggccgccct
     3241 cgacggccac cggcacgccc gagacgccgc tgcgccccgc gtgcagcttg agcgccaggt
     3301 agacgggggt ggcatcaacc cgcgccggcg tcacctggag ggcgccgccg gacggggtcc
     3361 acagccccag gtgagcccgg tcaacacggt cacccaggcg ggtcagtgcg ttgagcacac
     3421 cacacgcaaa tagcccggcg gcgaagctct gctcgggggc cgccccgggc ttctccacgg
     3481 tgaggctcca ttcatcgagg gcaaggcgaa ccggcctctc cgcgggcgcg ctggcggatg
     3541 ccgtggccgc caggcgctgc aactgcgctt ccaactgcgc cgcccctgcc accgcggccg
     3601 aataggccat cagcgggtcg tcggcgggct ccagcggccg ggaatagcgt accgaaaggt
     3661 aatccaactc gggggcggca gccgcaagct gagagcggct ccaggcgtcg cgtcccctgc
     3721 tctcaacgct caggccggcc acgcggatat tcgagtccac accgcgcatc gcctgcgcaa
     3781 actcgaccgc tcgcacggca tcgtggaagc cgtgcgccag gcttagcccg ccggggccag
     3841 ttccgttgcc ggcgagcgtc caggtgcgca cgccgaacgg ctcggtgcgc ccgttggccg
     3901 cgcgaatccg gccctgcgcc gactcggcga ggccgttgca atactccacc cagcgagctg
     3961 cctcgcgcgc ggcactttcg ccggcattca ccaccaggca gggctcggca cctactcgcc
     4021 ggcagaactc caggaactcg tgcgtgccaa catcgttcgt ctcccacgcg cctcccgcgc
     4081 ggttccagcg tgcggggcgc tggtcgcgcg gcccgatgcc atcctcccag tggtagcccg
     4141 cggccaggcg gctgcccggc cagcagatca tcgccgggtg gagggcgcgc gccgcctcaa
     4201 tcacatcttt gcgccagccc ccctcgctat ccgcgggcat cagcgacacg gcgccgatcc
     4261 agagcgtgcc tgcgccttcg aaggtgatcc ggagcgtcgc gccggccacg tcgcggcccg
     4321 gcttcgggaa ggtgaactcc tccgtgtgcc atcgggcgtc gggcagccgg atctggcgct
     4381 ccgccagcac ttcctcgccc gcgaggaggg tgattgagac cggaccgatc tccccttcct
     4441 cacgcgccac cagacggccc atcaggccta ttccctcgcg caataccagc ccgctctggg
     4501 cgatgccgca cgcctcgccc gcctgcgcgc gcgtgatccg ctgcgactgc accggcgcat
     4561 agaagatgcg attatccggc gcgaaggtga ctcccgcgcc ggctcccacc ggggtccacc
     4621 cgtccacgac gccggtctct cccgccgggc ccgcaaactt ccggttggcg agcatctcgg
     4681 cccacggccc gccgccagcg ctgcggccca ggcactccag cgtgtagctc gcccggaacg
     4741 ggctcagcgc cgggcgttcc gcgcggccat caacccgcac agtcgcctcc aggggcggca
     4801 gctcgcgctc cgcccgcggg atcaggcgga tatagtccac gtcgaaggtg atccccggcc
     4861 cattgaggaa ggtctttccg atgattccca ggaattgcgg gggccgaagg agcgtatacg
     4921 tgcccccgtc cacccagtct ccctcgccac ggcggaggcc tgccaggtag gtgcgtccca
     4981 ccttcgtgag gcggaggacc aggtcgcgcg cctcgcctcc caccttcatg tagccgctct
     5041 tccccgtcac ctccagccac gcctcgggcg cctgctgccc gccgggcagg cccgggccat
     5101 agaagatccc ccatgccagg agctcggcgt cactggtgcc caccatcagg ccggcgtgga
     5161 agttgcttcc cggatcgaac tcctgcagct gcacgcgcgc ctctagctcc caatcgcctt
     5221 caggcacgcg ggttctcagt tggggggctt cgtcgatttg ccccggctcg ttccagtggt
     5281 tgaagccgtt ctccttctgg ggaatgcgca cgcgtagcca gcccgggcgt tccgtgagtg
     5341 agtaggtggg gccctcgacc ggcacgcgcc aggtccagcg ggggtctagc gccgggccat
     5401 cgaagttctc ttcccatccc tgggcactgg cggcacccgc ggtcacgagc agcaggatca
     5461 agctgaagca ccacatggcg gtctccttcc cgggagggcc tcggaggcgc tctaccccac
     5521 cccgtgaggc ggccatgcct ccggcccatc gagaatgcaa cggcggcgcg gctggcatct
     5581 gggaagagac cgggctcccg ggttctgccg ggccggcgag gcgggcatct cccacgagag
     5641 ctgcctccga tatcaccgag tgcccccgcg cgcgaggcaa tccgcggcgg aaacatcggg
     5701 cgccagccac tctgggagat cggactcccg gggcgccgga tggtttcagg gcggtgaccg
     5761 atggcgtcag agaccgacgc tgcgcgcgac ctccttcagc gcctcggcgc tcttgcggaa
     5821 gccctccacc tcggcatcgc tcagcttcag ctccagtacg cgcaccaccc cggcgcgccc
     5881 gatcaccgtg gggaggctga ggcagatatc gtgaacgccg tactggccct ccatgaggct
     5941 cgacaccgtg acgaccgtgt gctggtcgtg caagatcatt ccgacgatcg cgtccaccgc
     6001 ggcgccgatg gcgtagtgcg tagcgccctt gcgctggatg atgtggtaag cggcgtcgcg
     6061 cacgttctcg aagatgacgc ggaggtcgcg gtcatcatga cagagcttgg tggcccggca
     6121 gaagtcggtc aggcgcatcc cggcgatgtt agccaggctc cagaccggca cctcggtatc
     6181 gccgtgctcg ccgatgatgt aagcgtgaac gctgcgcggg gccacctcga agtacttgcc
     6241 gaggctgtag cggaagcgcg ccgtgtccag cgaggtgccg gagccgaggc accggttggg
     6301 cgggaacccg gaaatcttcc acatggcgta ggtgaggata tcgaccgggt tggtcaccat
     6361 catcaggatg caatcctggt tgtgcgcggt gatcttcggg atgatctcct tgaggacctc
     6421 gatgttgttg cgcaccaggt cgatgcgcgt ttcgcccggc ttttgcgcca cgccggcgct
     6481 gatgatcacg atatcggcgc ccgcgcagtc ggagtagtcg ccggcttcga cgcgcacggt
     6541 gcccccgaag ggaagcccgt gagagatatc catcgcctca ccgatggcgc gctcgtggtc
     6601 ggcatcgatc agcacgatct ccgccgcggt gcccctcgtc atcatcgtgt aggcggtcgt
     6661 cgaaccgacg cggcccgcgc ccacgatcac cactttcgag ataggctcca tcgcctgcat
     6721 ctgtgccata gccgtcttcc ccttcacttt catcatagga caggcgttcc cggacgtcaa
     6781 cagccaggca caggaacgcc cgcccgcggg gctatcccta tccgcagatg gacgcagata
     6841 accagaatag ggtaactgtc gatgagggcg ttgacctccc ccacgccgcc ggcgacggca
     6901 gggctggccc attcccgccc tcttcagtgt cctccgcgag ccccaagcgg tagctcccct
     6961 tccctggggc cagcgcgggg ccagcggtag gggaggggta ggggttagga cgtccccttc
     7021 attgcgtggt atcgacgccg gcgcaggcaa aggccaatca gccccaggag caccgcgagt
     7081 gagagggagg ataccaggat cccctgctcc cacgagagcg gctcgtaggt gaagacgacc
     7141 tcgtgccgac cgggcgggac gcgcactgcg cgcgcaatca cgttcgcccg gtagatgggg
     7201 gtcaactcgc catccacgcg cgcgcgccac cccggatagt tgtagtcgct caggaccagc
     7261 cagccgccgc cggggctctc cacctccagg acaatccgct ccgcctcaga ggagacccag
     7321 cgcacctggc cgggcgcccg acccgtcgcc cgagccgatg cgccctcgac agcctctcgc
     7381 gcatcgagga gcaccgtctg tcggggatcg tggtccggcc gggcaaagcg ctcgaggaag
     7441 ccccgcaaac cgtctaccgc ctcggcagtg ggcgccaggt aggcgcgcgg caagaggtag
     7501 gggttgcgcc acagccggac ggaggcttcc cggcggccag ccgccgggaa gccctggatg
     7561 aggcgcgcgc cgcccggcga gagatcgtcc ggcgcggtga tgtagccggc gttgtaaagg
     7621 ccgagcaggc gcagccccag ccggatggca tccggcgact gcccggcgag aagacggttg
     7681 atctgggcat ccagccggcc cttatcggtg aagaagaagc cggtgtaggc agagtaatgc
     7741 ggcatcccct gaagcacgcc gaagttagga aggagcgtgt tcctcagcgg gtagaacgcc
     7801 tcgggatcgc gctgccaccc ccgcgagtgc tccagggcat cctgccaggg caccacctca
     7861 tgccaggtat agacgcggca ctggtcgcgg tcggcgccaa tcgctgccat aacgggcgcc
     7921 ggacccagcc atttgcgcgc gtccaccagc gcatactgtc gggaagcgtg agagagaagc
     7981 tctgccgcgg tgagagcgaa gcagaggccc acgagcgcgg gcgccagccg gaggcgccag
     8041 gtggcgaggc gactccgcgc cagatcgaag ccgtagccgg caagtgccgc cagcgcgaga
     8101 tcggtgacga gcaggaagcg cgccgggatg cgaaactcac ccatgccggg gaggtggcgc
     8161 cagaggaagg ggaagagcgg tgtctgcttc cccatagcga ggaggagcga cactacggcg
     8221 gctccagcaa ggagggccac gcgccggtcg cgccgccaga gcgcgccggt ggcatagagc
     8281 gccaggacca gggggagaag gcccacatag ccgcagttct cccagaagag aacggcctgg
     8341 ccgcccgcga agaaggagta ggtgttgcgc cccgggtcgc caaagaagaa gggcgacacg
     8401 aaggtgacca ggtgctgcca gggaagcgtg tagtaagtcg ccttctccag agaatccttg
     8461 gcagagcggt gcgcgagctg cgccagctcc caggtgggca gcaactgcac gcccgccagg
     8521 cccgcgccaa gcccagccgc cagcacgaac ccggcggcag cgagggccgc cgcgcggagg
     8581 gatcgcaggg ccagacgccc cggggaggat ggcgtcgcat ccactgaaac ctcgcgctga
     8641 agcacccgaa ggatcaggta gacgccggtg aagaggatgc tatagtaggc cgtctgcggg
     8701 tgtccggcca gatgctggag cgcctgtacc ggcgccagcg ccagcaagta ggcggggcgg
     8761 cgcgttcgcg cgtagcgctc gcacaggagc aggagcaacg gcagccactg ggcggcggcg
     8821 atcaggttca ggtgccggat gtgcccgaag aagaaccccg aaaacgcgaa aaccatcccg
     8881 gcgaagagcg ctgccacgcg gctttgctcc agctcgcgtg cgtacaggaa catcagcagg
     8941 cccgcgccaa ggtaggtgag caccagcgac aggttgatcg acgcgacgag cggaagccag
     9001 cccaggagca ggttgggcgg gtagaggaag cccccctggc cctcaccgtg gatcggaaag
     9061 ccggtgttca tcgcgtccgt ccagtggggg agggtgccac ggcgcagggc atcgcccagg
     9121 atccggcgca cggggtagtt gaaatcgagg aggtcgctgc ccgcgaccat ctcgctgacg
     9181 aacggcacct ggcgcaggag caacactcct ggaaactgca ggaaaagaag gaagagaagg
     9241 aaaagggctg ccgctgccac cggctgccgg cgcgcccaca cccgcgcgcc cgacagaatg
     9301 gcagatgcgc ccacggcaat cttggggaac aggggagcct cctttcgaca cccggaccag
     9361 ggcggggcag cctgtgccga gagccgcttc gccgcgtcgc ctctctggag gcgcgcacct
     9421 aacataccac ccgcccgagg gaatgtcaac cgattcttgt cctgcccaaa cgcttatgat
     9481 agaatggatt caatcgccgt ttggcacggg gaaggaggag cgcatgcgac ggttcgagga
     9541 actggccacc gaaggaatca acccggaaca cgcgcggctg gatgaactac ccacgctgga
     9601 gctggtcacg gcgatgaatc aggaggaccg cagcgtcgcc gacgcggttg ccgcggttct
     9661 tccggaggtc gctcgcgccg ttgatcttgt ggtgagcgcc ctgggaaacg gcggacggct
     9721 cttctacgta ggcgcgggca ccagcgggcg gctgggcgcg ctggatgccg cggagtgccc
     9781 tcccaccttc agtgtcagcc gcaacgtggt gcaggcgatc atcgccggag ggccggaggc
     9841 actgctccat tcggtggaag ccgtggagga ctccgaggag gggggtgcgg agcagatcac
     9901 cctggcttcg gtctccaacc tggatatcgt ctgcgggctc agcgcctgcg gcagcacccc
     9961 cttcgtcctt ggcgcaatgc gggcagcgcg cgagcgcggg gcgcgcacca tcgggatcgc
    10021 gtgcaaccgc ccttcggaga tggagcccct cgccgacgtg atgatcacgc cgctgaccgg
    10081 ccccgagatc ctctccggct ccacccggct caaggcgggc actgcccaaa agatgatcct
    10141 caatatgctc tctaccgccg ccatggttcg cctgggcaag gtgtatggta acctgatggt
    10201 cgatgtccac ccggtgagca agaagctggc ggaccgggcg cagcggatca tccgcgttgc
    10261 cacgggcgtc tccgaggagg aggcgcgcgc gctctttgag gaatcgggtg gccggcccaa
    10321 agtggcgatc gtaatgcaga agcgccactg caccccgaag cgagccgagg agctactggc
    10381 gcgcaataag gggtttgtca aggcggccct cgcggaccaa cgcccgtgaa cgcctggccc
    10441 cggatggcgg gaggtgcccc gcggctctcc cgccgggttc tgaccgggat actcctgggc
    10501 ctggccgtgc tggccgttcc cgccgcaccg acgcccaccg atggtcccgt catccgcgtg
    10561 ggcctcggcc agcgcgcgac ggcggtgcgc ctctcctctc gtgccgggct ccaggtgcag
    10621 ggccccgatg gcggcctcct cctggaggtc cgccgcgcgg tcaccctgca gccggtcacc
    10681 ctgcagcccg tggctccaca cgcgggcact cgccagccgg tcgctcgaga ggctggcgcg
    10741 cgccagccag gcgcgtcgaa tgccgatgcg cgccagccag gcgcgccgga cgcggctacg
    10801 ccagagtcac cggcgcacgg caccccggaa accgcgccgc cagagaccaa tgaggcgccc
    10861 atcgtgccag atcgcccggc cggccccctg ctctccgcaa ccgataccag cgatggcgag
    10921 gcgcccgcgc tggccatccc cttctccacc ctccgcatcg ttccatgcga ggaaggcgcc
    10981 cccctggacc taggcaagag gcggtatcgg ggcacgattg aggtgcgcgt ggacgcccgc
    11041 gggcgcctga cggtcgaacg tcgcagccgg cgggccaacg ggaaggc
//
LOCUS       DAOSCV010000019        10994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_3960_length_10994_cov_4.768352, whole genome shotgun sequence.
ACCESSION   DAOSCV010000019 DAOSCV010000000
VERSION     DAOSCV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_10994_cov_4.768352"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3378)
                     /locus_tag="PLY56_01005"
     CDS             complement(<1..3378)
                     /locus_tag="PLY56_01005"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ20088.1"
                     /translation="MSRQWIIGFAGILAALCLLAPGALADTTPQEVEEDQSLETKFVT
                     PHKAWARPYAGGRVRALFIVNGGNYSGSWFSIDTRLREPIELLQRFDMTGDAILTGSS
                     GRPDTFLGGTLGYQRAERLLSKPYDLYVFANLPMERFPAKLQYLVMEQVAKGAGLLCC
                     GAPASEFMVAKRQIAPLPDALRSGLPALDGKKPEEVIHAYQLGKGRGVWLKYDAWALT
                     PRQAFSYRRLAEYDYRMLLVGRAALWAVGREAPVSLSFGEGNGTVQSVSLASNDAKPL
                     AVKVALELRRAEDGQTWALGEVPVTLEPGKWAQVPVRLPKARAGDYFIDAIARGSRGI
                     VGCGAHAMTVSTPAGVERVELEKSFVERGESIGGKVVLRGTPPAGSTLRLCFRDSYHR
                     ILDQRDLRTEPGQTEYAFQYRAGGPDTILMRAQALLLASNGDEIEMKEASFTVPKRRR
                     GEFTFLQWDAPNDVPGYYAWRRLQEAGQNVCLIGSFSESKPHELLQACDASLVPYSTR
                     ILDPKDEFGYMQPTCWNHEPAVTEHVQKIVNNQLKRREHGVYVYSLGDEGVTLGCCVH
                     PSCIQAYRRYLAREYGTIDKLNASWGTTYKSFEEVDLLDHRDNMEQAALEKGLYARWY
                     DRQAFARYNLMQFSGRFVETYKRLDPHALTGFEGTGGFGDDYDTILGTNTFYSPYPGI
                     GDDILRSAAPRDLIRANWMGYSKTGDALSDAAWRMVMKEMNSVWFWMWTGIGSWRGYL
                     NPALDFWPATADLTEEMRPVREGLGDLLMRSEVTHSGIGIFYSVPSALSGRVENSREF
                     NGPENAHLTWTRLIYENGHDFRYLTSGMLKQGSLDKGGFKVLILPMAQAIAPEEAAAI
                     RRFVQAGGTIIADVRPGIYDGHCKPVLPGALDDLFGIQRTGRGKGVRGDIALKGRLGE
                     QAIDVTLRDTIRDPEVEPAGAEVMARAEDGSPLLLVNRAGAGRAILLNFQMSVANPEA
                     PEAMEARRLAKALLEVGGARAAITFSDPKGEPMPFTETRVWRNGDALVFGLWRQMQCA
                     WFGPKTGTIAGKPVPARVTLPEPRHVYDLRAGKYLGKVDRIDTRLRWGRASFYLALPY
                     PIRGVDLSVSQTAPAPGTTLFATIRLNIPAGSQERHAV"
     gene            complement(3462..4535)
                     /locus_tag="PLY56_01010"
     CDS             complement(3462..4535)
                     /locus_tag="PLY56_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20089.1"
                     /translation="MLRLVLTLLIVSLAASAHAAGNLAETTLVLPCRPAPAPITLDGT
                     LDPAEWAGAIEVSGFRVSGSPLLAPEQVTLRALYDKTSLYLGVRCLESQMAKVKAAVR
                     THDGPFWEDDAIEFFIDASHNHQNYWQFAATAGGARYENRDGDSAWNADWKLAVSREQ
                     DAWVVEAVVPFRELGLEPPAPGSLWGFNLCRERQAGGRLELYNWADVKRVFQSPGLFG
                     HLYFAPAGWQADAASVRDAARKAEGRETLIFVEGGYWRVRGRRAPEQRSYQMLLDLRA
                     RTTTGQMTELRALYRDRPGLKMREEFTRLDSRLAEVEALARTDKTVDALEWSRAAAFL
                     DSLQERVDAIYWHARLAILNERL"
     gene            4718..5773
                     /locus_tag="PLY56_01015"
     CDS             4718..5773
                     /locus_tag="PLY56_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20090.1"
                     /translation="MEKVRIAVIGCGGISAAHINGILAEPRAELVYCVDIDEEKARAR
                     AEKCGAKVATDYRSILDEVDAVDICTPPHLHAEMTVAAAEAGKHVLCEKIMAIDLDQA
                     EAMIEATDKAGVVFMIAFVLRYRREFMLLNEICRSGRLGQIHQAYAQTSMMLGIPPQK
                     WRQSVETFPMGCFLSHGCHYVDQLIWNVGEITEAASFGNAFTFHEWIPGGDDTSVAIF
                     RHKNGAVSAYCESWATPYPMTRLRIDVYGKEGSARVEYLPDARRVVEVCDKSGWNRVW
                     EFDPNKKELMDVFGGAKDMHGQISHFVECILDKKQPTTHGREGIKAMQVILAATEAEK
                     TGRIVNVQEFLAARARR"
     gene            5836..7080
                     /locus_tag="PLY56_01020"
     CDS             5836..7080
                     /locus_tag="PLY56_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20091.1"
                     /translation="MYPVHAEKVVITEWVAEDPRAWERARRLAAAFGSPEAPVVSAEE
                     LAALARERAWREDAGVRTGAARTHPVPDVVLNAFTFDPAVLAARNEAYPELRVWGLTG
                     GGAWSYRDSSVSRRTQGCVCQSAWEIHGAYGCLHACAYCHVGRVLNIMTNLESLAEHL
                     EAFMAEHPELKLYKFDNQTDTICFEPEYGASRVMVELFARQRDRFLMLYTKSANVEHL
                     LELDHRGQTLISWSLSARTGASLIEKGTPSMEARIAAMRACQDAGYGVRARLSPICPV
                     ADWRREGTELIEALFASVKPDLLTLDVLGWMNARQMMAAMDLSLFERRFRDFVLEKAK
                     ETPGPHRKHLFPHAWRAEILRHYVSEIRRVSPTTPVSICMETVEMWDELGADLGMTPD
                     HYACCCGPTSVPGHPLLRAQAA"
     gene            7765..8295
                     /locus_tag="PLY56_01025"
     CDS             7765..8295
                     /locus_tag="PLY56_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOJ20092.1"
                     /translation="MEGEVLVLNRDYEPLNICNLKRASVLVYLGKAEVLHVDGKTLTT
                     VNGSSTAPSVLRLRHQVRRPRPRLRLSRRSIFARDNYTCQYCGETGKEMTIDHVVPKR
                     LGGGDAWENLVACCRRCNGKKGDKTLQQAGMKLLREPRKPGFIPFISLSKFMQGARHD
                     VWRQYLPVYPELEGRD"
     gene            9202..10914
                     /locus_tag="PLY56_01030"
     CDS             9202..10914
                     /locus_tag="PLY56_01030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOJ20093.1"
                     /translation="MEQIYASVRKEEDPVRFIEAVFSILETRVDITERDLARIPKQGP
                     AIAVANHPFGGIEGLALIWLLRLVRPDVKVMANFLLGRIPQLRDSFILVDPFGRPESS
                     RANIRGLREAIRWVEEGGMLAIFPSGTVSHLHLRSLQVTDPKWSGTIARLFRHTKASA
                     LPVYFAGCNGPLFQMLGLAHPLLRTVMLPREMVNKRGRVIEARVGDPIPSRKLCAYTD
                     DEMVDLLRDRTYLLGMRARSQATPARRSIRRPKVAQLEPLVEAEPAEVLAEEVRSLPE
                     EQILVESKEHLVCYARANQIPHLLREIGRLREYTFRGAGEGTGKALDIDAFDEYYLHL
                     FVWNREKQEIMGAYRLGRTDEILPRHGLGGLYTQTLFRYHRELFNLMGPALEMGRSFV
                     RPEYQRSYAGLLLLWRGIGEYVCRHPDCKTLFGPVSISNGYQVISRQLIVAYLRQVEQ
                     MHPWSRFVRPRNPFQRAKPGLRRRALTYSALEDAADIDGISEVISHIEEDQKGVPILL
                     KNYLKLGACPLGFNVDPDFSDVLDVLVLVDLSKTDPKLLERYMGKDGAAGFLEYHGRR
                     GAAA"
BASE COUNT         1887 a         3575 c         3654 g         1878 t
ORIGIN      
        1 gacagcgtgt cgctcctggg atccagccgg gatgtttagg cggatggtag cgaagagcgt
       61 cgtaccggga gccggggcgg tctgagagac cgagagatcc actccgcgga tgggataggg
      121 gagagccagg tagaagctcg cccggcccca gcgcaggcgc gtgtcgatgc ggtcgacctt
      181 ccccaggtat ttgcccgcgc gcagatcata gacgtgacgc ggttccggga gagtgacgcg
      241 tgccggcacc ggcttgcccg cgattgtgcc ggtcttcggc ccaaaccagg cgcattgcat
      301 ctgacgccag agtccgaaca ccagggcatc gccgttgcgc cacacgcggg tctccgtgaa
      361 gggcatgggc tcgcccttcg ggtccgagaa ggtgatggcg gcccgggcgc ctcccacctc
      421 gaggagcgcc ttcgccagcc ggcgcgcctc catcgcttcc ggagcttccg gatttgccac
      481 cgacatctgg aagttgagaa gaatagcgcg cccggcgccc gcgcgattca cgaggagcaa
      541 ggggctgcca tcctcggcgc gcgccatcac ctcggcgccg gccggctcca cttccgggtc
      601 gcgaatcgta tcgcgcagcg tgacgtcgat tgcctgctcg cccaggcggc ccttcagcgc
      661 gatatcgccg cggacgccct tgccgcgccc ggtgcgctgg atcccgaaga ggtcatccag
      721 cgcgcccggg agtaccggct tgcagtgacc atcgtagatg ccggggcgca cgtcggcgat
      781 gatcgtgccg ccggcctgga cgaaccgccg gatcgccgcg gcctcctccg gggcaatcgc
      841 ctgcgccatc gggaggatga gcactttgaa cccgccctta tcgagcgagc cctgcttcaa
      901 catgccgctg gtgagatagc ggaagtcatg cccgttctcg tagatcaggc gcgtccatgt
      961 caggtgggca ttctccgggc cgttgaactc gcggctattc tcgacgcgcc ccgagagcgc
     1021 tgagggaacg gagtagaaga tcccgatccc gctgtgcgtc acttccgagc gcatcaggag
     1081 gtcgccgagg ccctcgcgca ccgggcgcat ctcctcggtg agatccgccg tagccggcca
     1141 gaagtcgagc gcgggattga gatagccgcg ccagctgccg atgccggtcc acatccagaa
     1201 ccagacgctg ttcatctcct tcatcaccat gcgccaggcg gcatcggaga gggcgtcgcc
     1261 ggtcttgctg tagcccatcc agttggcacg gatcaggtcg cgcggcgcgg cggagcgcag
     1321 aatatcgtcg ccgattccgg gataggggga gtagaaggtg ttcgtgccga ggatggtatc
     1381 gtagtcgtca ccgaagccgc ccgtgccctc gaaaccggtg agcgcatggg gatcgaggcg
     1441 cttgtaggtc tccacgaagc gcccggagaa ctgcatcagg ttgtagcgcg cgaacgcctg
     1501 tcggtcgtac caccgcgcgt acaggccctt ctccagcgcg gcctgctcca tgttgtcacg
     1561 atggtcgagg aggtccactt cctcgaagga cttgtaggtg gtgccccacg aggcgttcag
     1621 cttgtcgata gtgccatact cgcgggcgag gtagcggcgg taggcctgga tgcaggaggg
     1681 gtggacgcag cacccgaggg tcacgccctc atcgcccagg gagtagacgt aaacgccgtg
     1741 ctcgcgccgc ttcagctggt tgttcacgat cttctgcacg tgctccgtca ccgccggctc
     1801 gtggttccag caggtgggct gcatgtaccc gaactcgtcc ttcgggtcga ggatgcgcgt
     1861 gctgtaggga acgagcgaag catcgcacgc ctggaggagc tcatggggtt tggactcgga
     1921 gaagctcccg atcaggcaga cgttctgccc ggcctcctga agccggcgcc aggcatagta
     1981 gcccggcacg tcgttggggg cgtcccactg caggaaggtg aattcgcctc gccgccgctt
     2041 gggaacggtg aaggatgcct ctttcatctc gatctcatcg ccgttgctgg cgagaagcag
     2101 cgcctgcgca cgcatcagga tcgtatcggg cccgcccgcg cggtactgga atgcgtactc
     2161 cgtctggccc ggctcggtgc gcagatcacg ctgatcgagg atgcggtggt aagagtcgcg
     2221 gaagcaaagc cgcagggtgc ttcccgcggg tggcgtgccg cgcaggacca ctttgccacc
     2281 gatgctctcg ccacgctcga cgaagctctt ctccagctct acccgctcga ctccagccgg
     2341 cgtggagacc gtcatcgcgt gcgcaccaca ccctacgatt ccacggctgc cgcgcgcgat
     2401 cgcgtcgatg aagtagtcgc cggcgcgcgc cttgggcagg cgcacgggca cctgcgccca
     2461 cttgcccggc tcaagcgtta ccggaacttc acccagcgcc catgtctggc cgtcttccgc
     2521 ccggcgcagt tcgagagcga ccttcacggc cagtggcttc gcgtcgttcg acgcgagcga
     2581 gacgctctga accgttccgt tcccttcacc gaagctgagc gatacggggg cttcgcggcc
     2641 gactgcccag agggctgcgc gcccgacgag gagcatccga tagtcgtatt cggccaggcg
     2701 gcgatagctg aacgcctggc gcggagtgag cgcccaggcg tcgtacttca gccagacgcc
     2761 acggcccttg cccagctgat aggcatggat cacctcctcg ggcttcttcc catcgagggc
     2821 gggaaggccg gaacggaggg catccggaag gggagcgatc tggcgtttgg cgaccatgaa
     2881 ctcgctggcg ggcgctccac agcagagaag gcccgcgccc ttcgccacct gctccatcac
     2941 caggtattgg agctttgccg ggaacctctc catcggcagg ttggcgaaaa cgtacaggtc
     3001 ataaggcttt gagagcaggc gctcagcgcg ctgatagccg agcgtgccgc caaggaaggt
     3061 gtctggtctc ccggagctgc cggtgagaat ggcgtcgccc gtcatgtcga agcgctgcag
     3121 cagctcgatc ggctcgcgca ggcgcgtgtc tattgagaac cacgaaccgg agtagttgcc
     3181 gccgttgacg atgaagagcg cgcggacccg gccaccggca tagggccgcg cccacgcctt
     3241 gtgcggcgtc acgaacttcg tctccagcga ctggtcctcc tccacctctt ggggggtggt
     3301 atcggccagc gcgcccggcg cgagcaggca cagcgcggcg aggatgcctg caaacccgat
     3361 aatccactgt cggctcatgg cgaaacctcc aactttgatg ggacgaggcg tggggcgaac
     3421 gactggggcc aaaccgctct tccggcccgt gccctcattc cttagagtct ctcgttgagt
     3481 atggccagtc gcgcatgcca gtagatggca tcgacccgct cctgcaggct atccaggaag
     3541 gcggcagcgc gcgaccactc caaggcatcc accgtcttgt cggtgcgcgc cagcgcctcc
     3601 acttcggcga gacgggagtc caggcgcgtg aactcctcgc gcatcttcag cccaggccgg
     3661 tcgcgataga gcgcgcgcag ctcggtcatc tggcccgtcg tcgtgcgggc gcggagatcc
     3721 aggagcatct ggtaggaccg ctgctcgggc gcccgacgcc cgcggacccg ccagtagcct
     3781 ccctcgacaa agatgagggt ttctcggcct tccgccttcc gggcggcgtc gcgcacagaa
     3841 gcggcatctg cctgccatcc ggcgggtgcg aaatagagat gcccgaagag ccccgggctc
     3901 tggaagacgc gcttcacatc agcccagtta tacagctcca gccggccgcc agcctgccgc
     3961 tcgcgacaga ggttgaatcc ccagaggctt ccaggggcag gcggttcgag gcccagctcg
     4021 cggaagggca cgaccgcctc caccacccag gcgtcctgct cgcggctcac cgcaagcttc
     4081 cagtcggcgt tccaggcgct atccccatcg cgattctcgt agcgggcgcc gccagccgtc
     4141 gcggcgaact gccagtaatt ctggtggtta tgactcgcat cgatgaaaaa ctcgatggcg
     4201 tcatcctccc agaacggacc gtcatgggtg cgcaccgccg ccttcacctt cgccatctgc
     4261 gattcgaggc agcgcacccc gagatacagg ctcgtcttgt cgtagagggc acgcaacgtc
     4321 acctgttctg gcgcgaggag cggggagccg ctgacacgga agccggagac ctcgattgcc
     4381 cccgcccatt cggcaggatc cagcgtgcca tcgagagtga tcggggccgg cgcaggccgg
     4441 cacggaagca cgagtgttgt ctcggccagg ttgccggcag catgggcact ggcagcgagc
     4501 gagacaatca gaagggtgag taccaggcgc agcataagcg acctcccttg tgttgcgtcc
     4561 acattcaatc tccaccggag gagttccttc gtgcccttgc cggctttgct cgcttttctc
     4621 ggaggcagag cgggctcgcg caacaagaga ccaggaacca cgggggcgcg cgaggaatag
     4681 cgacccaacc ctattccccg gaagggcagg aagtatcatg gagaaggttc gcatcgcggt
     4741 cattggctgc ggcgggatca gcgccgcgca catcaacggc atcctggcgg agccgcgcgc
     4801 cgagctcgtc tattgcgtcg atatcgacga ggagaaggcg cgggcgcgcg ccgagaagtg
     4861 cggcgccaaa gtcgccaccg actaccggag cattctggac gaggtggacg cggtggatat
     4921 ctgcacgccg ccgcacctgc acgcggagat gacggtagcc gccgcggagg cgggcaagca
     4981 tgtcctctgc gagaagatca tggccattga ccttgaccag gcggaggcga tgattgaggc
     5041 cacggacaag gccggcgtcg tcttcatgat agccttcgtc ctccgctacc ggcgcgagtt
     5101 catgctcctg aacgagatct gccgcagcgg gcgcctcggg cagatccacc aggcctacgc
     5161 gcagacctcg atgatgctcg gcatcccgcc ccagaagtgg cgccagagcg tggagacctt
     5221 cccgatggga tgcttcctct cccatggctg ccactacgta gaccagctca tctggaacgt
     5281 gggcgagatc acggaagcgg cctccttcgg caacgccttc accttccacg agtggatccc
     5341 cggcggcgac gacacctccg tcgctatctt ccggcacaag aacggcgcgg tgagcgccta
     5401 ctgcgagagc tgggccaccc catacccgat gacgcgcctc cggatcgacg tctatggcaa
     5461 ggagggttcc gcgcgcgtgg agtacctgcc ggatgcccgg cgggtcgtcg aggtgtgcga
     5521 taagtcggga tggaaccgcg tctgggaatt cgatccgaac aagaaggagc tgatggacgt
     5581 cttcggcggc gccaaggata tgcacgggca gatctcccat ttcgtcgaat gcatcctgga
     5641 caagaagcag cctacgacgc acgggcgcga ggggatcaag gcgatgcagg tgatcctggc
     5701 cgcgaccgaa gcggagaaga cgggccggat cgtgaacgtg caggagttcc tggcggcgcg
     5761 ggcgcgccgg taacggcagg gcgtgacggc cgtaggccta tccgcgcggg cgcaaggcag
     5821 caaagggggc tcggaatgta cccagttcat gcggaaaaag tggtcatcac cgagtgggtc
     5881 gcggaggatc cgcgggcgtg ggagcgcgcg cggcgcttgg cggccgcgtt tggatctccg
     5941 gaggcgccgg tcgtctccgc ggaggagctg gccgcgctcg cgcgagagcg ggcctggcgc
     6001 gaagatgccg gcgtccgcac cggagccgcg cggacgcacc ccgtgccgga tgtggtgttg
     6061 aacgcgttca ccttcgaccc ggcggtgctc gcggcgcgca acgaggccta cccggagctg
     6121 cgggtctggg gcctgaccgg cggtggcgcc tggagctacc gcgacagctc ggtttcgcgg
     6181 cgcacgcagg ggtgcgtctg ccagtcagcc tgggagatcc acggcgcgta cgggtgcctc
     6241 cacgcgtgcg cctactgcca cgtcggccgt gtgctcaaca tcatgaccaa cctggagagt
     6301 ctggccgagc acctcgaggc gttcatggcg gagcaccccg agctgaagct gtacaagttc
     6361 gataaccaga cagacacgat ctgcttcgag ccggagtatg gcgcctcgcg ggtgatggtc
     6421 gagctgttcg cgcgccagcg cgaccgcttc ctgatgctct acaccaagag cgccaacgtg
     6481 gagcacctgc tggagctcga ccatcgcggg cagacgctca tcagctggtc gctttcggcg
     6541 cgaaccggcg ccagcctgat cgagaaaggc acgccctcga tggaagcgcg catcgccgcg
     6601 atgcgcgcct gtcaggatgc cggctatggg gtgcgcgccc ggctctcgcc catctgcccg
     6661 gtggccgact ggcggcgcga gggcaccgag ctgatcgagg cgctcttcgc ctcggtgaag
     6721 cccgacctgc tgaccctgga cgttctgggc tggatgaacg cccgccagat gatggcggcg
     6781 atggatctct cgctcttcga gcgccgcttc cgcgacttcg tcctagagaa ggcaaaggaa
     6841 actccagggc cccaccgcaa gcacctcttc ccccacgcgt ggcgcgccga gatcctgcgc
     6901 cactatgtga gcgagatccg gcgcgtcagc ccaaccacgc cggtctccat ctgcatggag
     6961 acggtggaga tgtgggacga actgggggcc gacctgggga tgaccccgga tcattacgcc
     7021 tgttgctgcg gcccgacgag cgttcccggc cacccgctgc tgcgcgccca agctgcttga
     7081 gaagcacgga agggtctctc gccgccatct ggcggggtgc tatcggtgca atggaccgac
     7141 gtatccggct gcgctctggg taaaacgaaa ggggagatat tgtgctgtgg catcgtcact
     7201 cgcgcctaag attccgacat aaggaatccg aagcctggga ggcattgcgc aacccgtcgc
     7261 gcggcgataa atcaccgcgc tgcttgcgag cgaagccctg ccgggctggg ttggggatac
     7321 tcccagatgt ctcaaaacgg cggtggctgg gaggagaagc cccgaggggg ctttgccgga
     7381 aagcagcgcg gagcttcgag ctccacgcgt gcgggcggcc ttctccttat ctcggaatcc
     7441 cagcacacgc gcgttgacat ttgccgcgcc tggctgctat aatattttcg gttgaatggg
     7501 ccggacgatc tggcccgaat gcctgggaaa gatgcgcact gtaatgcaga gacgagccat
     7561 gactattcat actcaacacc acggcggcgg tgatcgccgg ggggcggctc tgcgcgtgcg
     7621 tcgattagag gatcacttcc caccaccggg gttccggata gcccggatac gaatgcgcac
     7681 ctagagaagc ccgctcctcc aatacggacg tagcgggctt cttgcgttat tgcgtggaac
     7741 gtcgtgagag gagcgtcaac gaggatggaa ggggaggtac tggttttgaa tagggactac
     7801 gagccgctga atatctgcaa tctgaagcgg gcatcggtcc tggtgtacct cggaaaggcg
     7861 gaggtgttgc acgtggatgg caagacgctg accacggtca acggcagcag caccgctcca
     7921 tccgtcctcc ggctccgcca tcaggtgcgg cgacctcgcc caaggctacg cctctctcgg
     7981 cgcagtatct tcgcacggga taattacacg tgccagtact gcggcgagac cggcaaagag
     8041 atgacgatcg atcatgtcgt gccgaagcgg ttgggcgggg gcgacgcctg ggagaatctg
     8101 gtggcctgct gccgtcgctg caacggcaag aagggcgaca agacgctgca gcaggccggc
     8161 atgaagctcc tgcgtgagcc gcgcaagccg gggttcatcc cctttatcag cctatcaaag
     8221 ttcatgcaag gcgcacggca cgatgtctgg cgtcagtacc tgcccgtcta tccagaactg
     8281 gaggggcgtg actgataggg tgcctaaccg ctggggcgcg cgggcaatgc cggcgcgcct
     8341 gtgctcgccc cggtggtgtg ggtggcccgt gcaggtcgct gatgcccggg cgcgggcgtt
     8401 tgcgcccggg ccgcggcgcg tagggcatgc gatcccttcg ggagtgcggc gacacccggc
     8461 gtgccgagct ggaagctccg cgctgctttc cggcaaaacc ccttgcgggt ctccccctgc
     8521 cagcccgcgc cagcaccggt tgcccgcgtg cagcccagcc tagcctagcc cgacagggct
     8581 tcgtccacaa gcagcgcggt gatttaccac cgcgcgatcg gggtggggcg gttgttcacg
     8641 cctgcgcgtt ccccacgacc gccgcgcggt cgcctccttc cctggggccg gtgggggtcg
     8701 acgcccggat gcatcgcccc ccagatgaag cggtaccctt ccctaccatc tgcgcccatc
     8761 tgtggtcatc tgcggataag acgatctttc cccccggggc gcgacgaaaa agccgccgcg
     8821 ggaggcgcca atcgggtccc aatcggcctg gaatatggta tactgtgacg taatgcactt
     8881 tcccggcgat ggccgggatc tcttttggcc tgttacggga agcatccggg gctttggacc
     8941 gagccggccc atgtcgcgcg gggagcgtat tgcttctggt ttgtgtgaag gtgctcgccg
     9001 cgatcctccg tttccggcct cccacgggcg atccgcatcc gggtcggtcc gcgggtcatg
     9061 atcttggggt gcgacatgca gacgcatagt gaccaatcag agcggatctt taagcttgcc
     9121 ctccggtgca agaacccagt cggccagcgg cttttctccc acctggcccc ccccttggag
     9181 agcattgtcg ctctgagggc gatggaacag atctatgcca gcgtcagaaa agaggaggat
     9241 cccgttcgtt tcatcgaggc ggttttctcc atcctggaga cgcgggttga tatcaccgaa
     9301 agggatctgg cacgcattcc caagcaaggg ccggcgattg ccgtggccaa ccaccccttc
     9361 ggcggcatcg agggcctggc gctgatctgg ctgctgcgcc tggtgcgccc cgatgtgaag
     9421 gtgatggcca acttcctcct cgggcggatc ccgcagttgc gcgactcctt catcctggtg
     9481 gatccctttg ggcgtcctga gtcttcgcgc gccaacatcc gtggcctgcg cgaggcgatc
     9541 cgctgggtgg aagagggagg catgctcgcg atcttccctt caggcacggt gtcgcatctc
     9601 cacctgcgca gcctgcaggt gaccgatccg aagtggagcg gcaccatcgc gcgcctcttc
     9661 cgccatacca aggcgtccgc gcttcccgtc tacttcgccg ggtgcaacgg cccgctcttt
     9721 cagatgctgg gtctggcgca cccgctgttg cgcacggtga tgctcccgcg cgagatggtg
     9781 aacaagcgcg ggcgcgtgat cgaagcgcgc gtaggcgacc ccatcccctc acgcaagctc
     9841 tgcgcctaca ccgatgacga gatggtggat ctcctgcgcg accggaccta cctcctgggg
     9901 atgcgtgcgc gctcgcaggc gaccccggcg cgccgttcga tacgccggcc gaaggtggcc
     9961 cagctggagc cgctggtcga ggcggagccc gccgaggtgc tggcggagga agtccgctca
    10021 ctgcccgagg agcagatcct cgtggagagc aaagagcacc tcgtctgcta tgcccgggcc
    10081 aaccagatcc cgcacctgtt gcgcgagatc gggcgcctgc gcgagtacac cttccgcggc
    10141 gccggcgagg gtaccggcaa ggcgctggat atcgacgcct ttgacgagta ttacctccac
    10201 ctcttcgtct ggaaccgcga aaagcaggag atcatgggcg cctaccgtct ggggcgaacc
    10261 gatgagatcc tcccgcgcca tggtcttggg gggctgtaca cccagacgct cttccgctac
    10321 catcgggagc tgttcaacct gatgggtccg gcgctagaga tgggccgctc cttcgtccgg
    10381 ccggagtacc agcgctccta tgccgggctg ctcctcctct ggagaggcat cggcgagtac
    10441 gtctgccggc accccgactg taagacgctc ttcgggccgg tgagcatctc gaacggttac
    10501 caggtgatct cgcggcagtt gatcgtcgcc tacctccggc aggtggagca gatgcacccg
    10561 tggtcacgct tcgtgcgccc gcgcaacccc ttccagcgcg ccaagcctgg cctgcgccgg
    10621 cgcgcgctca cctattctgc cctcgaagac gccgcggata tcgacggcat ctcggaggtg
    10681 atctcgcaca tcgaggaaga ccagaagggc gtgccgatcc tgctgaagaa ctacctgaag
    10741 ctcggcgcct gcccgctggg cttcaacgtc gaccccgact tcagcgacgt cctcgacgtg
    10801 ctcgtcctgg tcgatctctc gaagaccgac ccgaagctcc tcgagcgcta catgggcaag
    10861 gatggcgccg cgggcttcct ggaataccac ggccggcgag gcgcggcggc atagcccgaa
    10921 gtattcgcca gaatacttca agagaagtct cttgtctggt gacacaccgt tgccgctaca
    10981 agcgcccgga ggca
//
LOCUS       DAOSCV010000020        10637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4115_length_10637_cov_3.585901, whole genome shotgun sequence.
ACCESSION   DAOSCV010000020 DAOSCV010000000
VERSION     DAOSCV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10637
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4115_length_10637_cov_3.585901"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLY56_01035"
     CDS             complement(<1..1153)
                     /locus_tag="PLY56_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ20094.1"
                     /translation="MAELARVDVANGVYWVEAPRADLYVLCGAPADSVKHLMKRGLIA
                     PREANGVHFETGPNAILLSDVLVQNGSFSNLAEFPVLHMLYRQGMLLPNHPNNTGAKP
                     LLIGSREQVRSQLEYIYRGNYGLVSEEELMACGISAAEARRMMQLKLAFAFGRIRPSH
                     ELVDTVVVGSDPVELRNGVFLRRRALNVFEFLYRGESLTVDLNLQPYQQYEAPYPLGH
                     YRVDRGYFSIVHSGEGDGWDPNRPAMASVLIFQGKPYLIDAGPNILHSIKSLGIGITE
                     VEGIFQTHAHDDHFCGLPTLMRSDRRIRYFATPMVRASVEKKLAALLGMDESHFQDFF
                     EPHDLKQEAWNEVDGLEVRPCHSPHPVETTVFVFRALGPSGYATYAHLAD"
     gene            1649..2272
                     /locus_tag="PLY56_01040"
     CDS             1649..2272
                     /locus_tag="PLY56_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ20095.1"
                     /translation="MKLGSQKRGRLTFQEEVEARSGINPARCYQCGKCTAGCPMTFAM
                     DLDPARVMRLVQLGQRDQVLQSHTIWLCASCETCTTRCPQEVDTARVMDALRILAREA
                     GFTQAEKGIAYANQGLLSSVRGRGRVHETMMVMLQNLRTLHPFRDAEKGPTMMLKGKI
                     SPFAHRSGRADQVRRLFENVERIEAQRAASAQKSAPEEHAPAAGGNE"
     gene            2269..3156
                     /locus_tag="PLY56_01045"
     CDS             2269..3156
                     /locus_tag="PLY56_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOJ20096.1"
                     /translation="MKYAYFPGCSLQGMAAEFDRSARAVCDQLGITLEEIPDWVCCGA
                     TPAHATSHLLSVALPAVTLAAVEQATQSGGPATMVAACASCYNRLRIASHEVKEDAAL
                     RADVEEAAQVKLAGTVEVRHLLDILRNDLGTEAIRNSVTRPLSGLKVACYYGCLLLRP
                     PKVVAFDDPNHPQIMEDLLTAAGAECVEWAYKTECCGASLSLARPDTVCKLVNDILRE
                     ARDAGADLIATACPLCQTNLDVRQLDVAKAYGTRYNIPAIYFTQLLGIALGLPEKSLG
                     LNRLIISPKPVLQKLQAAA"
     gene            3237..5231
                     /locus_tag="PLY56_01050"
     CDS             3237..5231
                     /locus_tag="PLY56_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOJ20097.1"
                     /translation="MARIGVFVCHCGENIARTVDCAKVAEFASRLPGVVHAVDYRYMC
                     SDPGQRLIQEAIREKNLTGVVVASCSPHMHEVTFRRAVQRAGLNPFLCEMANIREQCS
                     WVHQDREEATEKACELVHLMVEKVKWNQPLEPIRVPMTRKALVIGGGIAGIQAALDIA
                     NGGREVILVEKGPSIGGHMAQLSETFPTLDCSQCIMTPRMVEVKNHPRITLYTYSEVE
                     SVDGYIGNFEVKIRKKARSVDENLCTGCGECQTKCPQGKSKTTQIPNEFDMGLSRRPA
                     IFVPFPQAVPNVPVIDRAHCRYFEDGKCGVCKKVCPAGAVDFEQEDQVITETVGAIIV
                     ATGYDVMPASHYGEYGGGRYADVIDGLQFERLLSASGPTGGEPRRPSDGKVPETVVFV
                     ACAGSRDPAHGVGYCSKICCMYTAKHAMLYKHKVHHGQAYVFYMDIRAGGKNYDEFVR
                     RAIEEEGAVYLRGRVSRIYEKDGKLIVKGADTLSGSPVEIEADLVVLATAVQPRDGAE
                     ALAQMLKVPYDGYCFLSEAHPKLRPVETNSSGVYLAGACQAPKDIPETVAQASAAAGK
                     VLGLFSVEEMEREPTVSRINERTCIGCFDCERVCPFGAIERKEIRDRQGNLVKLVSHV
                     NPGVCAGCGACVTTCRSGSADLAGFSDEQVMAQIAALGVG"
     gene            5269..6633
                     /locus_tag="PLY56_01055"
     CDS             5269..6633
                     /locus_tag="PLY56_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ20098.1"
                     /translation="MMGDTFEPRIAAFVCNWCTYTGADLAGTSRLPYASNVRIIRLPC
                     TGRISPLFILKAFEQGADGVIVSGCHPGDCHYTAGNYHARRKYTVFRELLHFLGIEPE
                     RITFSWVSAAEGKKWADLVNEVTATVRRLGPRPAAWGEPIIGADGSVAVPALKPPAPI
                     RYEAPSEKFAAQTEALREKARELLESGRVEVVIGYGEGTLPGSVRPVFIKRPEDVERL
                     IWNEHCHHNLSVYAAREIAAHPGKVALVVKGCDELSIVGLIQENRFAAEDVVLIGMGC
                     HGMADAYGAVQIRCAACTVRTPSFASEVIGEATPAPAGTNPAAEVVAQLDALAPAERW
                     NFWQTQIQHCLKCYACRAACPLCSCDRCIVEKTQPRWIPPQADGPGNLSWSAVRAWHL
                     AGRCVGCGACMEACPAGIPLYLLNLKIAQVVKEAFDYEPGRDPEAAPPLTTYREDDKQ
                     EFIR"
     gene            6630..7691
                     /locus_tag="PLY56_01060"
     CDS             6630..7691
                     /locus_tag="PLY56_01060"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ20099.1"
                     /translation="MSRKALKAQSLRPLLEAVQSDAARVVAPVEEAGKRLFRPVETVE
                     AITFEEENTAWSFKEFLFPRTETLFHYRLKSAGVELAEPALSEGETVIFGARPCDAAS
                     VALLDAVFAPGGRKMPDGRVEPDDPFYPRRRAQTTIIGVSCAEPGPACFCTAVGLSPT
                     GTLGSDVLLTRVGEEYLVEASTEKGERFLEQYAQFLTETEATKSEVTAAAEAKIRRTD
                     PVRIDLDAPLPELFKSARLEEMARACLGCGACAFICPTCHCFDIADEGNAVSGSRCKF
                     WDSCGQACFTMHTSGHNPRPNQPSRYRNRLLHKLRYIPERFGVHGCVGCGRCALTCPV
                     NMDIYEVARAVEDERETRL"
     gene            7698..8537
                     /locus_tag="PLY56_01065"
     CDS             7698..8537
                     /locus_tag="PLY56_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOJ20100.1"
                     /translation="MANIYLPYLMTIAEVRDETPDVKSFRLEFQDEAARESFRFKAGQ
                     FALYSVFGQGECVFCIASPDTRPGYVECSFSYRWGGKVTTALSKLGVGDTVGFRGPYG
                     NWFPIDRMKGKNLVFIGGGIGMAPIRSIFETCLDQRAEFGDILLLNGARSVADMVYKP
                     QMEEWIARPDITCVKTVDPGGETPDWDGKVGLIPKVLEEVAPSSKDALALVCGPPILI
                     KFALQSLEKLGFTKEQVYTTLENRMKCGIGQCGRCNIGGCYVCKDGPVFSAAELDKLP
                     PEF"
     gene            complement(8770..>10637)
                     /locus_tag="PLY56_01070"
     CDS             complement(8770..>10637)
                     /locus_tag="PLY56_01070"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ20101.1"
                     /translation="PGAPKVHTETEIDFSNITAACDSLEAQGYPSTFSYLMFGIHEYL
                     YAPKWLVEKYRDEPDFFKVSWDGLKGGSRGNEHSLNWFHPAVREYIRDYLTRYATFCK
                     NEPRILFHEVAQEAYPDFTTSKGRRETGYGPHAVQAFRAYLKARYPSIEALNRAWGTK
                     YAGFDSIEPPPDAYAQRGREATPLVAEFEAFRDDAYIDYLKLIYDSLKAGDPNKPVVA
                     RHSGLLSAINGARIFETCDVLSYHRGAPQMQLMNLYLNSLNRYHRRGLGYMEDFWGLQ
                     DPRADDEVVQRRSLERHIAEECIWGRSLQMKWYSYTTGAYIFTYNGNWMNPRYDVTTM
                     RYCAPALAVAKQKMEKLDWALTHSEIPPQRVLVLQPSASMRNERPEQGVFAEILGIHR
                     LLYGNGILYELLPEEYFLNGRARLAEFDVVILPKARYLAADLQQALAKYVRDGGLLIA
                     CGEPGTHDELARPSGTLVRAVREASQQADWAKVEAAWQAAGPAAPETPMAIARCGQGE
                     WVACASMAGVAVENGGKALLERITRRTKREAWPVAGRLIVVPRVTEDGGRTLFLLNPD
                     LDAPVEEQVRVARAANRAVDLTVDGGYPIALKRDGEGVSFTVRLGPGESTAVWLE"
BASE COUNT         1846 a         3485 c         3516 g         1790 t
ORIGIN      
        1 tatcggccag atgggcatac gtggcataac cgcttggccc caatgcccgg aagacgaaaa
       61 ccgtggtctc tacggggtgg ggcgaatggc acgggcgcac ctccaggcca tccacctcgt
      121 tccatgcttc ctgcttcagg tcatgcggct cgaagaaatc ctgaaaatgg ctctcatcca
      181 tgccgaggag ggcggcaagc ttcttctcca ccgaagcgcg caccatcggc gtggcgaagt
      241 agcggatgcg ccggtcggag cgcatcagcg tgggaagccc gcagaagtgg tcgtcatggg
      301 cgtgcgtctg gaagatcccc tcgacctccg tgatcccgat gcccaggctc ttgatgctgt
      361 ggagaatgtt ggggcccgcg tctatcaggt agggtttccc ctggaagatg aggacgctcg
      421 ccatcgccgg ccggttcgga tcccacccgt cgccctcccc cgagtggacg atggagaagt
      481 agccgcggtc aacacggtag tggcccagag ggtatggggc ctcatactgc tgatagggct
      541 ggaggttcag atccacggtg agcgactcac cgcgatagag aaactcgaag acgttgaggg
      601 cgcgccgacg caggaagaca ccattccgga gctccaccgg gtcacttccc accaccacgg
      661 tgtcaaccag ctcgtgcgac gggcggatgc gcccgaaagc gaaggccagc ttcagctgca
      721 tcatccgccg cgcctccgcc gccgagatcc cgcaggccat cagctcctct tcggagacca
      781 gaccgtagtt gccccggtag atgtactcca gctgcgaccg cacctgctcg cgcgacccga
      841 tcaagagtgg cttcgcgccg gtgttgttcg gatggttggg gagcaacatc ccctggcgat
      901 agagcatatg gagcaccggg aactctgcca ggttggagaa gctgccattc tgcaccaaca
      961 catcggaaag gaggatggca ttcggccccg tctcgaaatg gacaccgttc gcctcacgcg
     1021 gagcgatcag cccccgcttc atcaggtgtt tcacgctatc cgctggcgcc ccacacaaca
     1081 cgtagaggtc tgccctgggt gcctctaccc aatacactcc attcgcgaca tcaaccctcg
     1141 ccagctcggc catacagccg atccccctct caggcagccg taatctttcc cgttttcgcc
     1201 ctccaagccc ttactccttt caccgtcccc ggcccttgac gagccagcgc cctctctgct
     1261 atagtgtgag cggacgacgt ggcagagatt gtccccgccg ccccatcccg cgcgccgcca
     1321 cgccgcgtga atagccggcg gatgcatccc gcagcccgcg cgctcccgct tccccgcgga
     1381 agtcggagac ccgcggaagg aagcgcttgg tcacagacca ccgccgcgtg cgagtacgct
     1441 ttggaaccac atccataacg acacggtcct ggagtatgcc cgcagcgcgg gtttcagatg
     1501 gggctcgttg ccgtggctcg ccatgcgtag cgcgtgcgag cgaccctggc gcgcacccgg
     1561 ccagaatgcg taacacgtag cccgccgtcg ggtcccgatg ccggctccgg ttcgccggtt
     1621 tcggattacg ctctcaaagg ttggcaagat gaagctaggt tctcagaaga gaggccgcct
     1681 caccttccag gaggaggtgg aggcacgttc cgggatcaac cctgcgcgct gctatcagtg
     1741 cgggaagtgt actgcggggt gcccgatgac ctttgccatg gatctggacc cggcgcgcgt
     1801 gatgcgcctg gtgcaactgg gccagcgcga ccaggtgttg cagagccaca ccatctggct
     1861 ctgcgcctcg tgcgaaacgt gcaccacgcg ctgcccgcaa gaggtcgata cggcgcgcgt
     1921 catggatgcc ctccgcatcc tggcccgcga ggccggcttc acccaggcgg agaaggggat
     1981 cgcctatgcc aaccagggtc tcctcagcag tgtccgcggg cgcgggcgtg ttcacgagac
     2041 gatgatggtg atgctccaga acctgcgcac cctccatccc ttccgcgatg cggaaaaagg
     2101 ccccaccatg atgttgaagg ggaagatctc cccattcgcc catcgctccg gccgcgccga
     2161 tcaggtgcgc cggctttttg agaacgtgga gcggatcgag gcgcagcgcg ccgccagcgc
     2221 gcaaaagtcg gcgcccgagg agcacgcccc cgcggcagga ggcaacgaat gaagtacgcc
     2281 tacttccccg gatgctcgct gcagggcatg gccgccgagt ttgatcgctc cgcgcgcgcg
     2341 gtgtgcgacc aacttgggat caccctggag gagatcccag attgggtttg ctgtggcgcc
     2401 acgcctgcgc acgccaccag ccacctcctc tccgtggcac tgccggcagt aaccctagcg
     2461 gccgtcgaac aagccacgca atcgggcggc ccggcaacga tggtggctgc gtgcgcctct
     2521 tgctacaacc gcttgcgcat cgccagccat gaggtgaagg aagacgccgc gctccgcgct
     2581 gatgtggaag aggcggccca ggtgaagctc gccggcacgg tggaggtgcg ccacctgctc
     2641 gatatcctgc gcaacgacct gggtaccgag gcgatccgca actcagtcac tcgccccctg
     2701 tccggattga aggtggcgtg ctactatggc tgcctgctgc tccgcccgcc caaggtggtc
     2761 gccttcgatg acccgaacca tccgcagatc atggaggacc tcctcacagc ggcgggcgca
     2821 gagtgcgtgg agtgggccta caagacggag tgttgcggcg ccagcctcag tctcgcgcgc
     2881 ccggatacgg tgtgcaagct ggtcaacgac atcctgcgcg aggcgcgcga tgccggcgca
     2941 gatctgattg ccacggcctg tcccctgtgc cagacgaacc tggacgtgcg tcagttggat
     3001 gtggccaagg cgtacggcac gcgctacaac atcccggcca tctacttcac gcagcttctc
     3061 ggcatcgccc ttggcctgcc ggagaagtcg cttgggctaa accggctgat catcagcccg
     3121 aagcccgtcc tgcagaagct gcaggcggcg gcatagccgg cgggcaacgc gcgaccggca
     3181 tttgagagcc gcgttcacgc ggctctcgaa acgcgggaaa aggaaacgag ttctcgatgg
     3241 ctcgcatcgg tgtctttgtc tgccattgtg gtgagaatat cgcgcgcacg gtggattgcg
     3301 cgaaggtggc ggagttcgcc tcgcggctcc ccggggtcgt gcatgccgtg gactaccgct
     3361 acatgtgctc cgatccgggc cagcggctga tccaggaggc gatccgggag aagaacctca
     3421 ccggcgtagt cgtcgcgtcg tgcagcccgc acatgcacga ggttaccttc cggcgggccg
     3481 tgcaacgcgc cgggctcaat cccttcctct gcgagatggc caacatccgc gagcagtgct
     3541 cctgggtgca tcaggaccgc gaggaggcca cggagaaggc gtgtgaactg gtccacctca
     3601 tggtggagaa ggtgaagtgg aaccagcccc tggaaccgat tcgggtgcca atgacccgca
     3661 aggcgcttgt gatcggtggc ggcatcgccg ggatccaggc cgcgctcgat atcgccaacg
     3721 gcgggcgcga ggtgatcctg gtcgagaaag gcccctccat cggcgggcac atggcgcagc
     3781 tctcggagac cttccccacc ctcgactgct cgcaatgtat catgacgccg cgcatggtgg
     3841 aggtgaagaa ccacccgcgg atcacgctct acacctactc cgaggtggag tcagtcgatg
     3901 gctacatcgg caacttcgag gtgaagatcc gcaagaaggc gcgctcggtg gacgagaacc
     3961 tgtgcaccgg ctgcggcgag tgccagacga agtgccccca gggcaagtcg aaaaccaccc
     4021 agatccccaa cgagtttgac atgggcctca gccggcggcc agccatcttt gtgccgttcc
     4081 cgcaggcggt gccgaacgtg ccggtcatcg accgcgcgca ctgccgctat ttcgaggatg
     4141 gcaagtgcgg cgtctgcaag aaggtctgcc ccgcgggcgc cgtggacttc gagcaggagg
     4201 atcaggtcat cacagagacc gtgggcgcca tcatcgtggc tacaggctat gacgtgatgc
     4261 cggcttccca ctacggcgag tatggcggcg gccgctacgc cgacgtgatc gacggcctcc
     4321 agttcgagcg cctgcttagc gcctcgggcc cgaccggcgg cgagccgcgc cgcccctccg
     4381 acggcaaggt gccggagaca gtcgtcttcg tcgcctgcgc cggctcgcgc gacccggcgc
     4441 atggggtggg ctactgctcg aagatctgct gcatgtatac ggccaagcac gcgatgctct
     4501 ataagcacaa ggtgcatcac ggccaggcct acgtcttcta catggatatt cgcgcgggcg
     4561 gcaaaaatta cgacgagttc gtgcgccgcg ccatcgagga ggagggtgcc gtctacctcc
     4621 gcggccgcgt ctcgcgcatc tatgagaagg acggcaagct gattgtcaag ggcgcggata
     4681 cgctcagcgg ctctcccgtc gagatcgagg ccgacctggt ggtgctcgcg accgcggtgc
     4741 agccgcgcga tggcgccgag gcgctggcgc agatgctgaa ggtgccctac gacggctatt
     4801 gcttcctcag cgaggcgcac ccgaaactgc gcccggtaga gaccaactct tccggcgtct
     4861 atctggccgg cgcgtgccag gcgccgaagg atattccgga gacggtggcc caggcgagtg
     4921 ccgccgcagg caaggtcctc ggcctcttct ccgtagaaga gatggagcgc gagccgaccg
     4981 tttcgaggat caacgagcgc acctgcatcg ggtgtttcga ttgcgagcgc gtctgcccgt
     5041 tcggcgccat cgagcgcaag gagattcgcg accgccaggg caatctggtg aagctcgtct
     5101 cgcacgtcaa cccgggcgtc tgcgccggtt gcggggcgtg cgttaccacc tgccgcagcg
     5161 gcagcgccga tctggccggc ttcagcgacg agcaggtcat ggcccaaatc gcggctttgg
     5221 gagtcggttg agtggcggca gccagaaggc aaaggtgcat aaaggtacat gatgggtgat
     5281 acgtttgaac cgaggatagc ggcgttcgtg tgcaactggt gcacctacac cggggcagac
     5341 ctggcaggca ccagccggct gccctacgca tccaatgtgc ggattatccg gctcccgtgc
     5401 accgggcgca tcagcccgct cttcatcctg aaagcgttcg agcagggcgc cgacggcgtg
     5461 atcgtctccg ggtgccaccc gggcgattgc cactataccg ccggcaacta ccatgcccgg
     5521 cgcaagtaca ccgtcttccg cgagctgctc cacttcctcg gcattgagcc agagcggatc
     5581 accttctcgt gggtctccgc cgccgagggg aagaagtggg cggatctcgt gaatgaggtg
     5641 acggcgaccg tgcgccggct cgggccgcgc ccggcggcgt ggggcgagcc gatcatcggc
     5701 gcggatggtt cggtcgccgt gcccgcgctg aagccgcccg cgccgattcg ctatgaggcg
     5761 ccgtcagaga agttcgcggc gcaaaccgag gcgctgcgcg agaaggcgcg cgagctgctg
     5821 gaatccggcc gggtggaagt ggtgatcggc tatggcgagg gcacgctgcc cggcagcgtc
     5881 cgccccgtct ttatcaagcg cccagaggat gtggagcgcc tgatctggaa cgagcactgc
     5941 caccacaatc tgagcgtcta tgccgcccgc gagatcgctg cgcaccccgg caaggtcgcg
     6001 ctcgtggtca agggatgcga cgagctgagc atcgtcggcc tgatccagga gaaccggttt
     6061 gccgccgagg acgtggtcct aatcgggatg gggtgccacg gcatggccga cgcctacggc
     6121 gcggtgcaga tccggtgcgc cgcatgcacg gtgcgcacgc catccttcgc cagcgaggtg
     6181 atcggcgagg cgaccccggc acctgccggg accaatcccg cggcagaagt ggtggcccag
     6241 ctcgacgcgc tcgcgcctgc cgagcgctgg aatttctggc agacgcagat ccagcactgc
     6301 ctgaaatgct atgcctgccg cgcggcctgt ccgctctgct cgtgcgaccg gtgtatcgtg
     6361 gagaagactc aaccccgttg gattccaccg caggcggatg ggccgggtaa cctgtcgtgg
     6421 agcgccgtgc gcgcctggca cctcgcgggc cgatgcgtag gatgcggcgc gtgcatggag
     6481 gcgtgccccg cgggcatccc gctctacctc ctcaacctga agatagcaca ggtggtcaaa
     6541 gaggcgtttg actacgagcc gggacgcgat cctgaggcgg cgccgccgct caccacctat
     6601 cgcgaggacg acaaacagga gttcatccgt tgagccgaaa agcactcaag gcacaatcat
     6661 tgcggccctt gctcgaggcc gtccaaagcg acgccgctcg cgtggtggcg ccggttgagg
     6721 aggccgggaa gcgcctcttc cgtccagtcg agacggtcga ggcgatcacc ttcgaggagg
     6781 aaaacaccgc ctggtccttc aaggagttcc tcttcccgcg gacagagacg ctcttccact
     6841 atcgtctgaa gagcgcgggc gttgagctgg ccgagccggc gctgtccgag ggagagacgg
     6901 tcatcttcgg ggcgcgcccg tgcgacgcgg cgagcgtggc gctgctcgat gccgtcttcg
     6961 cccccggcgg gcggaagatg cccgacggcc gggtggagcc agatgacccg ttctacccgc
     7021 ggcggcgcgc gcagacgacg atcatcggtg tctcgtgtgc cgagccgggc ccggcgtgtt
     7081 tctgcaccgc ggttggtctc tcccccaccg gcaccctggg aagcgacgtg ctcctcacgc
     7141 gtgtgggcga ggagtacctg gtggaagcgt ccacggagaa gggcgagcgc ttcctggagc
     7201 agtacgccca gttcctgacg gagaccgagg caacgaagtc ggaagtgacc gctgccgccg
     7261 aggcgaagat ccggcgcacc gatccagtgc gcatcgatct agacgcgccg ttgccggagc
     7321 tgttcaagag cgcccgcctg gaggagatgg cgcgtgcctg cctggggtgc ggggcgtgcg
     7381 cgttcatctg ccccacctgc cactgcttcg acatcgccga tgagggcaac gcggtgagcg
     7441 gcagccgctg caagttctgg gactcctgcg ggcaggcgtg cttcaccatg catacctccg
     7501 ggcacaaccc gcgcccgaac cagccctcgc gctaccgcaa tcgcctgctc cataagctgc
     7561 gctacatccc ggagcggttc ggcgtgcatg ggtgcgttgg ctgcgggcgt tgtgccctca
     7621 cctgcccagt gaacatggat atctacgaag tggcaagggc tgtcgaggac gagcgcgaaa
     7681 cgcgcctgtg agcaatcatg gctaacatct atctccctta cttgatgacg attgccgagg
     7741 tccgcgacga gaccccggat gtaaagagct tccgcctgga gtttcaggat gaggcggcgc
     7801 gcgagtcgtt ccggttcaag gcggggcagt tcgcgctcta ctccgtcttt ggacagggtg
     7861 agtgcgtctt ctgcattgcc agtccggata cgcgccccgg ctacgtggag tgcagcttca
     7921 gctaccggtg gggcggcaag gtgaccacgg cgctgagcaa gctgggcgtg ggtgacacgg
     7981 tcggcttccg cggcccctat ggcaactggt tccccatcga ccggatgaag gggaagaacc
     8041 tcgtcttcat cggtggcggc atcggcatgg cgcccatccg ctccatcttc gagacatgcc
     8101 tcgaccagcg cgccgagttc ggcgatatcc tgctcctcaa cggcgcgcgc agcgtcgccg
     8161 acatggttta caagccgcag atggaggaat ggatcgcccg tccggacatc acctgcgtca
     8221 agacggtgga cccgggaggc gagacgccgg attgggatgg gaaggtcggg ctgatcccca
     8281 aggtgctcga ggaggtggcc ccgtccagca aggatgccct cgccctcgtc tgcgggccgc
     8341 cgatcctgat caagttcgcg ctgcaatcgc tggagaagct cgggttcacc aaggagcagg
     8401 tttacaccac ccttgagaac cgaatgaagt gcgggatcgg gcagtgcggc cggtgcaaca
     8461 tcggcgggtg ctacgtctgc aaggacggtc ctgtcttcag cgccgccgag ctcgacaagc
     8521 tcccacccga gttttgagat agcgccagaa ggagcgcgga gatggaagct ccgcgctcct
     8581 gcccggcaaa gccccttcgg ggctcatcct cccaacccac tgccaccccc ggttagccgc
     8641 gggttcaggc tagcccagcc cgggcgaagg ttaaccaccg tgcgccgggt tgcgcgccgg
     8701 cattgatgct ttggaggcca gttcgggaag gggcgagggt cgcccggaac gggccctcgc
     8761 cccctctctc tattccagcc agaccgcggt gctctcgccc gggcccaggc gcaccgtgaa
     8821 ggagactccc tcgccgtcac gcttcaaggc gatcggatag ccaccgtcga ccgtcaaatc
     8881 gacggcgcgg tttgccgccc gggcgacgcg gacctgctcc tcaaccgggg catcgaggtc
     8941 cggattgagc aggaagagcg tccgcccgcc gtcttccgtc acacggggca ccacgatcag
     9001 ccggccggcc accggccacg cctcgcgctt cgtccggcgg gtgatccgct ccaggagcgc
     9061 cttcccaccg ttctccacgg cgacgcccgc catcgacgcg caggccaccc actcaccctg
     9121 gccgcagcgg gcgatggcca tcggtgtttc cggagccgcg gggccggcag cctgccaggc
     9181 cgcttccact ttcgcccaat cggcctgctg agatgcctcg cgcacggcac ggacgagggt
     9241 gcccgagggc cgggccagtt catcgtgagt gcccggctcg ccacaggcga tgaggaggcc
     9301 accatcccgg acgtatttcg ccagggcctg ctggaggtcg gcggcgagat agcgcgcttt
     9361 cgggagaatc accacgtcga actccgccag gcgcgcccgg ccattcagga agtactcctc
     9421 cggaagcagc tcgtagagga ttccgttccc gtagaggagg cggtggatac ctagaatctc
     9481 ggcgaagacg ccctgctccg ggcgctcgtt gcgcatgctg gccgaaggct ggagcaccag
     9541 gacgcgctgc gggggaatct cgctgtgcgt gagcgcccag tcgagcttct ccatcttctg
     9601 cttggccacg gcgagcgctg gcgcgcagta gcgcatcgtg gtcacgtcgt agcgcgggtt
     9661 catccagttg ccgttgtagg tgaagatata ggcgcccgta gtgtaggagt accacttcat
     9721 ctggagagag cgcccccaga tgcactcctc ggcgatgtgc cgctccaggc tgcggcgctg
     9781 gaccacctcg tcatcggcgc gcgggtcctg cagcccccag aagtcctcca tgtagcccag
     9841 cccgcgccgg tgataccggt tgagcgaatt gaggtagagg ttcatcagct gcatctgggg
     9901 cgcgccgcga tggtacgaaa ggacgtcgca cgtctcgaag atccgcgccc cgttgatcgc
     9961 gctgagcagc ccgctatgcc gggccaccac cggcttgttg ggatcgcccg ccttgagcga
    10021 gtcgtagatc agcttcaggt agtcgatgta ggcgtcgtcg cggaacgcct cgaactcagc
    10081 caccaggggc gtagcctccc gcccgcgctg cgcgtaggca tcgggcggcg gctcgatgga
    10141 gtcgaacccg gcgtactttg tcccccacgc ccggttgagt gcctcgatgc tcggatagcg
    10201 cgctttcaga taggcgcgga acgcctgcac cgcgtggggg ccatagccgg tttcgcgccg
    10261 gcccttgctg gtggtgaagt cgggataggc ctcctgcgcc acctcatgga agaggatgcg
    10321 cggctcgttc ttgcagaagg tggcataccg cgtcaggtag tcgcggatat actcgcggac
    10381 cgccgggtgg aaccagttga gcgagtgctc gttgccccgg ctgccaccct tgagaccatc
    10441 ccacgagacc ttgaagaagt ccggctcgtc gcgatacttc tccaccagcc acttcggggc
    10501 gtagaggtac tcgtggatgc cgaacatcag atagctgaag gtgctggggt agccctgtgc
    10561 ctcgagcgaa tcgcacgcgg cggtgatgtt gctaaagtcg atctccgtct cggtgtgcac
    10621 cttgggcgcg cccgggg
//
LOCUS       DAOSCV010000021        10385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4243_length_10385_cov_3.747435, whole genome shotgun sequence.
ACCESSION   DAOSCV010000021 DAOSCV010000000
VERSION     DAOSCV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_10385_cov_3.747435"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="PLY56_01075"
     CDS             <1..127
                     /locus_tag="PLY56_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ20102.1"
                     /translation="AMKIPVKMLFPLVFLVLPALFVVILGPAVIQMLGSLGAGGR"
     gene            129..1073
                     /locus_tag="PLY56_01080"
     CDS             129..1073
                     /locus_tag="PLY56_01080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ20103.1"
                     /translation="MDHTGEPLIVLRGRRVLVADDEPESIDLVSTILVSEDYQVLSTT
                     HGDEVLHQARLHQPDLILMDLEMPGMDGLSVAQALRHDERTQHIPVIFVTASAALENK
                     LRALREGANDYITKPFHREELLARVNVALRLKALQDALRERNRLLAELATHDELTRLH
                     NRRYLEQRLSEEVPRAKRYRLPLSCLMLDLDHFKRINDTYGHPAGDAVLRQVADILRT
                     SVRSVDVVGRYGGEEFLIILPQTGAEGARVLAERIRQRVEQELFDIGGQTIHCTTSIG
                     VAAASDGDVPDAGHLIAEADRALYHAKVTGRNRVSSAP"
     gene            complement(1174..2478)
                     /locus_tag="PLY56_01085"
     CDS             complement(1174..2478)
                     /locus_tag="PLY56_01085"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOJ20104.1"
                     /translation="MHAILEVDRIRCGYTAAPVLEEVSFSLAPGELVAVIGPNGSGKS
                     TLLRAVSRTLRPQSGEVRVEGREIYSATPQWVARRIAVVPQETRVDFAFTVREIVLMG
                     RLPHLGRFATESATDFAIAREALQRTGVLKLEDRSILGISGGERQRVMIARALAQQTP
                     ILLLDEPTAHLDINYQIEIMRLVNTLRADGARAILVVLHDLNLAAQFCDRLLILHRGR
                     IFADGPPERVLTRENVHLAYGADVLVRRHPQTGRPYVVAEAGRGLPQFAEAEERPLVH
                     VICGAGTGEAVFESLLERGWRVTAGVVNTGDSDQVAAKRLNVRYVDEAPFTAITEDTH
                     HAHLRFIEDADAVLLTPFPMGPANLRNLDAAHHALSLGKPVWLLDAAGARARDFSGGA
                     AGDRYEALLTAGACPIASIAELPVPAASNSASGDRSGTQPNG"
     gene            complement(2723..3415)
                     /locus_tag="PLY56_01090"
                     /pseudo
     CDS             complement(2723..3415)
                     /locus_tag="PLY56_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(3360..4556)
                     /locus_tag="PLY56_01095"
     CDS             complement(3360..4556)
                     /locus_tag="PLY56_01095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOJ20105.1"
                     /translation="MTAREVEVVREVLFTERAVPPPPPITAQSAILVDADTGKVLYER
                     HAHERRPMASTTKIMTALLALEHARMNDTVVVSEHASKTPYTGLYLKPGEKLTVGDLL
                     WALLLRSANDACVAIGEHIAGSEEKFVEMMNARALEMGLKNTHFKNPHGLHAEDHYST
                     AYDLAQMTRVAMQHPLFAQIVARKRKRIDRSIEKQDVVVVNKNRLLFRWDAVDGVKTG
                     YTRQAGHCLVASASQDGWRLISVVLKSGNSWGDSRTLLEHGFRQYERVLVARKGTPLL
                     QVRVVNGLQDSVQAMLVKDLSVVVPRGTKVDVRTEVTPGMARAPLWRGDSLGIVVARQ
                     GETELGRSLLLAADPVEPAPKPLLSRFLFFGLAGFTGLLALSVVVRHGRKAAQSTGRR
                     RRRLAP"
     gene            complement(4674..5171)
                     /gene="scpB"
                     /locus_tag="PLY56_01100"
     CDS             complement(4674..5171)
                     /gene="scpB"
                     /locus_tag="PLY56_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ20106.1"
                     /translation="MEDRELSEVLEALLFASGEPVSLDRLIDATEEPRERVARVLEEM
                     RAQPRGGLLVAEVAGGWMLCTRPEYAPFITRLLRVQPQRLSRAALETLAIIAYRQPIT
                     GPEIEAIRGVSIDGVLGTLLARGLVCEAGRKQAPGRPILYKTTEEFLRYLGIRDLAEL
                     PPLEE"
     gene            complement(5182..5919)
                     /locus_tag="PLY56_01105"
     CDS             complement(5182..5919)
                     /locus_tag="PLY56_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ20107.1"
                     /translation="MVELDAFSGPLDLLLHLIQKQEIDIYDIPIARITDQYLAYLQLL
                     EALDIEVAGEFIVMAATLMEIKSRMLLPPAPKQEDEEEGPDPRAELVQMLEEYRRYKE
                     AAEQFKKRSEVWGRVFPRGSELPAEIASLVPEAGFANLSVPALLEALQQVLLEAETEH
                     GARTVQREKVTVRMKIAELWHRLSVHPEGVTFRSLFDRPFTRVDVIATFLAVLELLRL
                     GRIVVCQAHAFAELHIQKVQAAADATA"
     gene            complement(6125..7648)
                     /locus_tag="PLY56_01110"
     CDS             complement(6125..7648)
                     /locus_tag="PLY56_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ20108.1"
                     /translation="MSEFSARYGERVGLERRKQFGQFFTPEPIARWMVRWVLAGGAGR
                     VYDPAFGLGAFHQAAGEIAEGVAFEGSEVDPVILGFWRARGDENGAVVRQEDYLRAWG
                     RQWPAIVCNPPYRRFQKFLGREEVFAAFEEHLGVKLSGYTNIASAFLLKSISELCDGG
                     RLAYILPLEFLNTGYGAVVKRQMLARGQLHAIIRLDCEKAAFPDVVTSVGILLFEKRA
                     PRFPTRFYVVSRLDALETVLDTPPVNSIPPEELRPGEKWLRHFEAGTLPPRSPHLVPL
                     STYGTFSRGIATGANAFFVLRPSQVTALGLAPEEVVPCITRSVQIRDPLFTESEFRRL
                     VEEDAPVYLLRAGDPPSAAAACYIRRGEAEGYHRRYLTRFRDPWYRLERRRPAPILFG
                     AFSRDGYKVIRNHSQALNLTCYHGFQPAGQGARYVERLFLYLLSRVGRETVALNVRQY
                     GAALGKYEPNDLNQALVPSMEWLDRLDEEQAAVEIEHARHGYLSSEMEALFETLIDP"
     gene            complement(7677..8723)
                     /locus_tag="PLY56_01115"
     CDS             complement(7677..8723)
                     /locus_tag="PLY56_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20109.1"
                     /translation="MQAPIGCAVIGYGPMHNFGWAHSAWIDATPELRLVAVCDCDPAR
                     TAAAKDAFPHIRTYNDVAQVWEDREIQLVSIVTPHYTHCPLALQAFDAGKHVVVEKAM
                     CLNVAEATAMVEASQRAGRMFAVHHNRRHDGNYRRIKEIVSSGAIGEVFHIELTAGSY
                     SEFRHGWYSEKEKSGGAFYFWGPHAVDWVLDLVPQRITGVNGFFHKLVWHNISNEDQV
                     RAIIRFEGGAVADVTWSHIAAIGKPLWRILGTKGAILDTGAGGNVGYEKQIHGPSGGS
                     LTLVTVGEDGRKEEKVPYLESDWEQYWQDVADHLLRGGPVPVPGTVGRRVIGVFEAAE
                     RSSRTGQTEQVPYE"
     gene            complement(8843..9802)
                     /locus_tag="PLY56_01120"
     CDS             complement(8843..9802)
                     /locus_tag="PLY56_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HOJ20110.1"
                     /translation="MREISYAAAIGEALREEMARDESVFVIGEDIGPVRQAEDLFAAF
                     RERRVWQTPISEAGFTGLAAGAAASGLRPVVEIMYCDFVGVAMDQICNQAAKMHLMSG
                     GQVKVPMVIKTPAGCGTREGGHHSGSLEAWFIHSPGIKVVMPATAYDAKGLLKSAIRD
                     DGLVLFIQHRLLHPLTQQVPEGEWIVPLGKADVKRRGQEITVVSYSYALTKVLAAAEE
                     LAGSIDVEVVDPRTLTPLDVETILESVAKTGRLLVVHEAPAQGGAGAEIVRQVVERGF
                     DLLKAPPRVLGGKHVPMPYSPPLEDACIPQVADIIRTIREMVE"
     gene            complement(9845..>10385)
                     /locus_tag="PLY56_01125"
     CDS             complement(9845..>10385)
                     /locus_tag="PLY56_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOJ20111.1"
                     /translation="VSVVFFGDGGANQGTLLESLNLAALWKLPVLFVCENNLWANQTP
                     VALSLPVEDVAPRAAAFGIPGEIVDGQDVLAVYHLAGEAVSRARSGGGPTFIEAKTFR
                     FEGHCGVSRGHENPEQCAEWRKRDPILLFEQRLASEGTVTPEEQRAIRTAVLAEIDEA
                     EAFATSSPFPDPEELSAFL"
BASE COUNT         1821 a         3598 c         3217 g         1749 t
ORIGIN      
        1 ggccatgaag atccccgtga agatgctttt cccactcgtc tttctcgtcc tgccggcgct
       61 cttcgtggtg attcttggcc ccgcggtgat ccagatgctg ggcagtctgg gagcaggagg
      121 tcgctgagat ggaccacaca ggcgaacccc tcatcgtact tcgaggacgg cgcgtgttgg
      181 tagctgatga cgagcctgag agtatcgacc tggtgagcac catcctggtg agcgaagact
      241 accaggtcct ctcgacaacc cacggcgacg aggtactcca ccaggcccgc cttcaccaac
      301 ctgatctcat cctgatggat ctggagatgc ccgggatgga cggtctcagc gtggcgcagg
      361 cactccggca cgacgagcgc acccagcata ttccggtgat cttcgtgacc gcctcggccg
      421 ccctggagaa caagctccgt gcgctgcgcg agggcgccaa tgactacatc acaaagccgt
      481 ttcaccgtga ggagctactc gcccgcgtca acgtggccct gcgtctcaag gcgttacagg
      541 atgcgcttcg agagcgcaac cggctcctcg ccgagctggc tacccacgat gagctcaccc
      601 gactgcataa ccgccgctac ctcgagcaac gcctctccga ggaggtgccc cgggcaaagc
      661 ggtatcgcct gcccctctcc tgcctgatgc tcgatctcga tcacttcaag cggatcaatg
      721 acacatatgg ccatccagcg ggcgatgcgg tcctccgcca ggtcgccgac atcttgcgca
      781 cctccgtgcg ctcggtggac gtggtgggcc gttatggcgg cgaagagttc ttgatcatcc
      841 ttccacagac aggtgccgag ggcgcccgtg tgctggccga gcggattcgc cagcgcgtcg
      901 agcaggagct cttcgacatc ggcgggcaaa ctatccactg cacgaccagc atcggtgtgg
      961 ccgcggcgtc agatggggat gtgccggatg ccggccacct gatcgccgaa gccgaccgcg
     1021 ccctctacca tgccaaggtg acgggtcgca atcgggtctc ctcagcgcct tgaatccggt
     1081 ccatccggca tccaggcgtg tcaggcgatg gaacggacgg atagcagatg ggggagcagc
     1141 ctctccgcca cgcgtctatc tccggccttt cactcaccca ttcggctggg ttccagagcg
     1201 gtcaccggat gcgctattgc tggccgcagg caccggcagc tccgcgatcg atgcgatggg
     1261 gcacgcgccg gcagtgagga gtgcctcata gcgatctcct gcggcgcctc cgctgaagtc
     1321 gcgggcccga gcgcccgctg catccagaag ccacaccggc tttcccagcg agagggcgtg
     1381 atgcgccgcg tccaggttgc gcaggttcgc cgggcccatc gggaagggcg tcaggagcac
     1441 cgcgtccgcg tcctcgatga agcgcaggtg agcgtggtgc gtgtcctcgg tgatcgccgt
     1501 aaagggcgcc tcgtccacgt agcgcacgtt caggcgcttc gcggccacct gatcgctatc
     1561 gcccgtgttg acgacgcccg cggtcacgcg ccatccgcgc tccaggaggc tctcaaagac
     1621 ggcctcgccc gtgcccgcgc cgcagatcac atggaccagc ggcctctcct cggcttcggc
     1681 aaactgcggc aggccgcggc cggcctccgc gaccacgtag ggccggccgg tctgcgggtg
     1741 ccgccgcacc agaacatccg cgccataggc cagatgcaca ttctcgcgcg tgagcaccct
     1801 ctccgggggg ccatccgcga agatccggcc ccgatgaagg atgagaagcc ggtcgcagaa
     1861 ctgagcggcc aggttgaggt catgaagcac gacaaggatc gcgcgcgcgc catcggcacg
     1921 cagagtgttc accaggcgca tgatctcgat ctggtagttg atatcgaggt gcgctgtcgg
     1981 ctcgtcgagg aggaggatgg gtgtctgttg cgccaacgcg cgggcgatca tcacccgctg
     2041 gcgctcgccg ccgctgatcc ccagaataga gcgatcctcc agcttcagca cgccggtacg
     2101 ctggagcgcc tcgcgggcaa tcgcgaaatc ggtggcgctt tccgtcgcga agcggccaag
     2161 gtgggggagc cggcccatga gcacgatctc gcgcacggtg aacgcaaaat cgacacgcgt
     2221 ctcctggggc acgacggcaa tccgccgcgc cacccactgg ggcgtcgccg agtagatctc
     2281 gcggccttcc actcggacct cgcccgattg cgggcgcagg gtacggctca ccgcgcgcag
     2341 cagcgtcgat ttcccggagc cgttcggacc aatgacggcc accaattcgc cgggcgcgag
     2401 agagaaagaa acctcctcca acaccggagc cgcagtgtag ccacagcgaa tccggtctac
     2461 ctcaaggatt gcgtgcatcg cgtggcctac cgctcatccg gccatcccgg cgtggcgcga
     2521 gcctgccccc tgccccatca ccgctctgtc cactcccgtc tcgctctttg ggagcagcgg
     2581 agtggcttgc gaggtttttg gccggagcac gcgccgcgcg tctctgtgga gctttcccgg
     2641 ggcgggccgc agcgcctcct tgcgggcgcg gcatggccgc ctgcggagtt acttcgcctt
     2701 tggccatgcg catcagcgca tgcacttcct tctgggtgag atagcgccac gcgccgagcg
     2761 gcagcttgcg cacgctgacc ggtccgatgg cgacgcgccg cagatagcgc accggatgcc
     2821 caaccgcatc gagcatccgg cgcacctggc gcttgcggcc ctcatgcagc gtgatctcca
     2881 acacagcgcg atcctcgccc gcgtagagta gccgcacctg agcgggcgcg gtcagtccat
     2941 cctccaatcg gaccccggtg cgcagtgctt ccaacgcctt ttcgtcggga acgccctgaa
     3001 cgtgcgcctg atagatcttc ggcaccccat gccggggatg ggtaagcaac tgcgtgagct
     3061 ccccatcatt cgtgacgatc agcgcgcctt cggtatcctt gtccagccgg cccactggat
     3121 gcacgggcac gtcaagatcg cgcagcagat ccatcaccgt gcgcccggca tgaggatcgg
     3181 cgcgggtggt gacgtagcct ccgggcttat tgaggagcac gtacgtcttg cgccgcggga
     3241 tcgtcaccgg gcgacccgcg acgctgatcg cgtgtttctc cgggtcaacc ttcacgccca
     3301 tctcggtgac ggtcttcccg tcgacggcca cttggcctgc ggcgatcagc cgctccgctt
     3361 cacggcgcga ggcgacgccg gcggcggcca gtactttgtg cagcctttct tccatggcgt
     3421 accactactg agagggcgag aagaccggtg aacccggcca atccaaagaa caggaagcga
     3481 gagagaaggg gctttggcgc cggctccacc ggatcggcgg caaggagcag actgcgtccc
     3541 agctcggtct cgccctgacg ggcgaccaca atgccgaggg aatcgccgcg ccagagcggg
     3601 gcgcgcgcca tgcccggggt cacctccgtg cgcacatcaa ccttcgtgcc acgcggcacc
     3661 accactgaaa ggtctttcac caacatcgcc tgaacggaat cttgaagccc gttcacgacg
     3721 cgcacctgta acaggggcgt gcccttccgg gctaccagga cgcgctcgta ctgccggaag
     3781 ccgtgctcga gcagcgtgcg cgagtcgccc cacgagtttc cactcttcaa cacaacagag
     3841 atcagccgcc atccatcctg ggaagcggac gcaaccaggc aatgcccggc ctggcgcgtg
     3901 tagcccgtct tcacgccatc cacggcatcc cagcggaaga gcaggcggtt cttattcacc
     3961 accaccacat cctgcttttc gatagagcgg tcgatccgct ttcgcttgcg cgccacgatc
     4021 tgtgcgaaga gcgggtgctg catcgccacg cgcgtcatct gagcgagatc atatgccgtc
     4081 gagtaatgat cctcggcgtg gaggccatgg gggttcttga agtgcgtgtt cttcaggccc
     4141 atctccagcg cccgggcgtt catcatctcg acgaacttct cctcgcttcc cgcaatgtgc
     4201 tcgccgatgg cgacgcacgc atcattcgcg gaacgcagga ggagcgccca gaggaggtca
     4261 cccaccgtca gtttctcgcc cggcttgagg tagagcccgg tgtagggcgt cttcgaggca
     4321 tgctcgctca ccaccaccgt gtcgttcatc cgggcgtgct cgagcgcgag gagggcggtc
     4381 atgatcttcg tcgtcgatgc cataggccgg cgctcatggg catgccgctc atagaggacc
     4441 ttcccggtat ccgcatcgac gaggatggcg ctctgggcgg tgatcggcgg cggaggaggc
     4501 accgcgcgct cggtaaacag gacttctcgc accacctcga cctcgcgtgc tgtcatgcgc
     4561 ctttcggcgc gcgggcgcgc aacaagaagc gccggagcgg cggctaacag cagcagccag
     4621 ggccagagcc ggcgcctacg acgtcttggg gcgaacaacc tgtctcttgc catctattct
     4681 tccaagggtg gcagctcggc cagatcccgt atgccgagat accgcaaaaa ctcctccgtt
     4741 gtcttgtaga ggatcgggcg accgggagcc tgtttgcgcc ctgcttcgca gaccaaccct
     4801 cgggccaata acgttcccaa cacgccgtcg attgaaaccc cgcggatcgc ttctatctcc
     4861 ggacccgtga ttggctggcg gtaggcaata atggccagcg tctccaatgc cgcccgcgag
     4921 aggcgctgag gctgcactcg cagaagtcgg gtgatgaagg gcgcatactc cggacgcgtg
     4981 caaagcatcc agcctccggc gacttcggcc accaggagcc cgccccgcgg ctgggcgcgc
     5041 atctcctcca gcacgcgagc cacgcgctcc ctcggctcct ccgtcgcatc tatcagccgg
     5101 tccagtgaca ccggttcgcc cgaggcaaag agaagggctt ccagcacctc actcagctct
     5161 ctatcttcca tattcactca ctcaagcggt cgcgtcggca gccgcctgga ccttttgaat
     5221 gtgcagctcc gcgaaggcgt gcgcctggca gacgacgatt cgcccaaggc ggagcagctc
     5281 cagcacggcc aggaaggtcg cgatgacatc cacccgggtg aaggggcgat cgaagagcga
     5341 ccggaaggtg accccctccg gatgcacgct gagacggtgc cacagctcgg cgatcttcat
     5401 gcgcaccgtc accttctcac gctgaacggt gcgcgcgccg tgctcggtct ccgcctccag
     5461 gagcacctgc tgcaacgctt cgagcagcgc cggcacggag aggttggcaa accctgcctc
     5521 ggggaccaga ctcgcaatct ccgccggcag ctcgctcccg cgcgggaaga cgcgccccca
     5581 cacctccgag cgcttcttga actgctcggc ggcctcctta tagcgccggt actcctcgag
     5641 catctgcacc aactcggcgc gagggtcggg accttcctcc tcatcctcct gcttgggcgc
     5701 cggcggcagt aacatgcgtg acttgatctc catcaaagtc gccgccatga cgatgaattc
     5761 gccggcaacc tcaatgtcga gggcttccag aagctgcaga taggcgaggt actggtcggt
     5821 gatgcgggcg atcgggatgt cgtagatatc aatttcctgc ttctgaatca ggtgcagaag
     5881 cagatccagg gggcccgaaa acgcgtccaa ctcaaccaca taagccaaag ttccgcctcg
     5941 ctccgccgat tggcgcccca agaaaagaga ggctcacctg cccgctgcag gaaagccctg
     6001 gaggtcccct acctccttgt ccattatacc gaagcgggcc gtagcctgtc aaacaaagcg
     6061 gcatgcccat cggggttccg agaggaggcc ggaccagtgt tctttcctgc cctcgggccg
     6121 gcagtcaggg atcgatcagc gtctcgaaga gggcttccat ttcggaggag aggtagccgt
     6181 gccgcgcatg ctcgatctcc acggccgcct gctcctcatc gagccgatcc agccactcca
     6241 ttgagggcac cagcgcctgg ttgaggtcgt tcggctcgta ttttcccaga gcggccccgt
     6301 actggcgcac gttgagcgcc acggtctccc tccccacccg cgataagagg tagaggaaga
     6361 gccgctccac gtaacgcgct ccctgcccgg caggctggaa gccatgataa caggtcaggt
     6421 tcagcgcctg ggagtggttg cggatcactt tgtagccatc gcgggagaag gcgccgaaga
     6481 ggatcggcgc cggccggcgc ctctccaggc gataccaggg atcgcggaaa cgcgtcaggt
     6541 agcgcctatg gtaaccctct gcctccccgc gccggatgta gcacgccgcc gccgcagacg
     6601 ggggatcgcc cgcgcgcagg aggtaaaccg gggcatcctc ctcaaccagg cggcgaaact
     6661 cgctctcggt gaagagtgga tcgcggatct gcacgctgcg cgtgatgcat ggcacgacct
     6721 cctcgggagc cagtcccagt gccgtgacct gcgagggccg aaggacaaag aaggcgttcg
     6781 cccccgtggc gatcccgcgg ctaaacgtgc cgtaggtgga gaggggcacc aggtgcggcg
     6841 agcgcggtgg gagcgttcct gcttcaaagt ggcgcagcca tttctcgccg ggccgcagct
     6901 cttcgggcgg gatgctgttc accggaggcg tatccagcac cgtctccaag gcatccagcc
     6961 gggaaaccac atagaatcgc gtagggaaac gcggcgcgcg cttctcgaag aggaggattc
     7021 ccacgctggt gaccacgtcc gggaacgccg ccttctcgca atccagccgg atgatggcgt
     7081 gcagctgccc acgcgccagc atttgccgct tgaccacggc gccatacccg gtgttcagga
     7141 actccagcgg gaggatgtag gccagtcttc ccccgtcgca cagctccgag atcgacttga
     7201 gcaggaacgc cgaggcgatg ttggtgtagc cggagagctt cacccccagg tgttcctcga
     7261 acgccgcgaa cacctcctcg cgcccgagga acttctgaaa ccgcctgtag ggcggattgc
     7321 agacgatggc cggccactgt cggccccacg cgcgcaggta gtcctcttgg cgaacgaccg
     7381 cgccattctc atcccctcgc gcacgccaga atccgaggat caccggatcg acctcggagc
     7441 cctcgaaggc aacgccctcg gctatctctc ccgccgcctg gtggaacgcg cccaacccga
     7501 acgcaggatc gtacacccgc cccgctccgc ccgccagaac ccatcgcacc atccatcgcg
     7561 cgatgggctc cggggtgaag aactgcccaa actgcttcct gcgctccagg cctacgcgct
     7621 cgccataccg ggcggagaat tcagacatta cgggggtctt ccgtggcgtt tcgggcctac
     7681 tcgtacggca cctgctctgt ctggccggtc cgggaggagc gttcggccgc ctcgaagacg
     7741 ccgatcaccc ggcggccaac agtgcccggc acaggaacgg ggccaccgcg caggaggtgg
     7801 tccgccacgt cctgccaata ctgctcccag tcggactcca gatagggcac cttctcctct
     7861 ttgcgcccat cctcgcccac ggtcaccaga gtgaggctac ccccggaggg accatgtatc
     7921 tgcttctcat agcccacatt gccgccggcg cccgtatcga ggatcgcccc cttcgtgccg
     7981 aggatccgcc acagcggctt gccaatcgcg gcgatgtgcg accacgtcac gtcggccacg
     8041 gcgccgcctt cgaagcggat aatcgcgcgc acctggtcct cgttcgagat gttgtgccag
     8101 acgagcttgt ggaagaaacc gttgacgcct gtgatccgtt gcggcaccag gtcgagcacc
     8161 cagtccaccg cgtgcggccc ccagaagtag aacgcgccgc cggacttctc cttctccgag
     8221 taccacccgt ggcgaaactc tgagtagctc cccgccgtga gctcgatatg aaacacctcg
     8281 ccaatcgcgc cgctgctcac gatctccttg atgcgccggt agttgccatc atggcggcgg
     8341 ttgtggtgga cggcgaacat gcgcccggca cgttgtgagg cctcaaccat cgcggtcgcc
     8401 tcggcgacat tgaggcacat cgccttctcc accaccacgt gcttgcccgc atcgaacgcc
     8461 tgcaacgcga ggggacaatg cgtgtagtgg ggcgtgacga tagagacgag ctggatctcg
     8521 cggtcttccc acacctgcgc cacgtcgtta taggtgcgaa tatgcggaaa ggcatccttc
     8581 gccgccgccg tacgtgccgg gtcgcagtca cacacggcca ccaggcgcaa ctccggcgtg
     8641 gcatcgatcc acgccgagtg cgcccatccg aagttgtgca tcggcccata gccgatgacc
     8701 gcgcagccga taggggcttg catctctttc ctccactcgc aacagggccc gatcccgggc
     8761 gtgcgctgcc aattccccgc gctcgccagg cagcgagaag ccatgccttg ccaggaagag
     8821 cgcgctagct tcgcctgggg cgttactcga ccatctcgcg gatcgtccga atgatatcag
     8881 cgacctgcgg aatgcaggcg tcctccagcg gcgggctgta gggcatcggc acgtgcttgc
     8941 ccccgagcac cctcgggggg gctttcaaga gatcgaagcc tctctcgacc acctgccgga
     9001 cgatctccgc gccggcgccg ccctgggccg gcgcctcgtg gacgaccagt agccggccgg
     9061 tcttcgccac cgactccagg atcgtctcca catcgagggg tgtcagcgtc cgcgggtcca
     9121 ccacttcgac atcgatgctc cccgcgagct cttcggccgc ggcgagcact ttggtgagcg
     9181 cgtaggagta ggagacgacg gtgatctcct ggccgcgccg cttgacatcc gctttaccca
     9241 gcggcacgat ccactcccct tccggcacct gctgagtcag gggatggagc aggcggtgct
     9301 ggatgaacag caccaggccg tcgtcgcgga tcgccgactt cagcaagccc ttggcatcgt
     9361 aggcggtggc gggcatcacc accttgatcc cgggcgaatg gatgaaccac gcctccaggc
     9421 tgccactgtg gtgcccgccc tcgcgcgtgc cgcatccggc aggcgtcttg atcaccatcg
     9481 gcaccttcac ctgtccgccg gacatcaggt gcatcttcgc cgcctggttg caaatctgat
     9541 ccatggcgac gccgacgaag tcgcagtaca tgatctcgac gaccgggcgc aggccggaag
     9601 ccgccgcacc cgccgccagg ccggtgaatc ccgcctccga gatcggcgtc tgccacaccc
     9661 ggcgctcgcg aaacgccgcg aagaggtctt ccgcctgccg caccgggccg atatcctcgc
     9721 cgatcacgaa gacgctctcg tcacgggcca tctcttcccg gagggcttcg ccaatcgccg
     9781 ccgcgtaact gatctctcgc atcatcgctc tccccgcgcg ccagaagcgc cacagaacgc
     9841 cggatcagag aaaagccgag agctcctcgg gatccggaaa cgggctgctc gtggcgaacg
     9901 cctccgcctc gtcaatctcc gccagcaccg ccgtccggat cgcgcgctgc tcttctgggg
     9961 tcacggtacc ttcgctggcc aggcgctgct cgaagaggag tatcgggtcg cgcttgcgcc
    10021 actcggcgca ctgctccggg ttttcatgcc cgcgtgagac gccgcagtgg ccctcaaacc
    10081 ggaacgtctt cgcctcgatg aaggtggggc cgccgccgga gcgcgcccgg ctcaccgcct
    10141 cgccggcgag gtggtagacc gcgagaacat cctggccatc gacgatctcg ccggggatgc
    10201 caaacgccgc cgcgcgcggc gccacatcct ccaccggaag cgagagcgcg accggcgtct
    10261 ggttggccca caggttgttc tcgcagacga agaggaccgg cagcttccag agcgcggcga
    10321 ggttgagcga ctccaggagc gtgccttgat tggcgccgcc atcgccgaag aagacgaccg
    10381 agacg
//
LOCUS       DAOSCV010000022        10329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4268_length_10329_cov_3.970119, whole genome shotgun sequence.
ACCESSION   DAOSCV010000022 DAOSCV010000000
VERSION     DAOSCV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4268_length_10329_cov_3.970119"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..2574)
                     /locus_tag="PLY56_01130"
     CDS             complement(370..2574)
                     /locus_tag="PLY56_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ20112.1"
                     /translation="MRKPPERRRLRVVIAVALLTIGTARFLPLLAGQVLITSLTPLCD
                     QWHVNMPFAFLLAEALREGRLPVWTDRIGGGYPLLALGEAGVLYPPHLLLYRLLPPVT
                     AYNVNVILAVLTAAACQYWLCRDLGRSRAASLLGGLAYAWSGFFVLHVATLHSAQAAA
                     WIPLLLLCVRRGVRAATPASWIGAGVVLAIQITTGYPQIVYYSALAAAFLAIWELGWG
                     EDRGAPGGPAGKVVNGVGGALVVLAVALGLSAAHWLPGVELTRQSGRSGGISFERATE
                     FSFPPANLLTFVAPYVHGNPATGGEMGFSERGHFWDNGYVGLIPLFLALYAAAYGLRR
                     KGPIRFFVFLLLFSLLIVLGRHTPFFRVVWHVLPGMSYFRFPSRFMVIACLSLALLAA
                     YGLDQLRARLLAAPGRDSRRRWAVLAGMLFAISFVDLWIYAARFHRFADAAAWLTLPK
                     SARLLHTVASPSARYYTFGYAGVQLEATRRARGWKGDLGPYLEQKEVLAPNTGAFYGL
                     SCFNFFGGLDTRRGTATYALLCDDRNTLGRTSTILESFPRTLALLGVTHVVTPYLLAI
                     PGLREEMAVKTGNSKQPVRVYAVEDARPQIWIPDQARSVAGGREAFRTLLEARWSAIE
                     GAPEFRNFSGEVLAWEAGPDWARARIRMRGTGVVIHNQRHYPGWRVLLDGEPAPLLRA
                     NYLMQGVAVPEGEHTLEFRFDSPSFRAGATISLLSMALLAAGCILTRLRGKR"
     gene            2705..4750
                     /locus_tag="PLY56_01135"
     CDS             2705..4750
                     /locus_tag="PLY56_01135"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOJ20113.1"
                     /translation="MPGRYVPWAGLLFLCAFAFYIATLAPGLMPGDTGDMVIAAASLR
                     EAHPPGYPLYAMIGKLFVALPIGDIPYRANLMAAFLGGLSVALVFLALLLLTRRPLAS
                     LAGALLFAFNYLFWTYFTLAETFCLNNFTLALTAFLALLWRERHRQEPGGRASRRLLV
                     LLAFVAGVSMGNHHTMVLTYPGLFLFVAVVDRSVLRPARLAALAGWFAFGLALVYAYL
                     PIAALVHHDHHWEDSSTLSGLLRLFLRAKYGTFQLAPGDGKPVTPPEQAASYFGALAT
                     QYLWPGFVLALVGLAALWKRDRAALLHSGGGFLFGVLFILTANIDPRPTAYDAATLER
                     FYQLPNFFFLLWTGAGLGVAERWLEGICADISSRAPIGKAIPAILLLALPAALLAVNY
                     GKANLRGNLLYPRFMTNIFDSLPKNAVFFVWSDTVGMGADYLTDIEKRRPDVAVIKVG
                     ILGAEPYRSTVRRKYPHLRWPDLAPDAPFSMGRFIQHNIDRYRIFLDGLPMKLTFPVI
                     RYGLIYEVRPQGSVPSAAEVLDLNEELWKRFDLRQVDTSLYPANSMCYSVVVFYYLYH
                     RFTMASECNYFGRYDAALRHLEACSQMDPKGYVARSAPWQRQKAVAHIGLNQLDQAVT
                     HLDLARKEDPSDAETYRLLSLAYQKKGDQAQADYYLDEYEIRRRAEEQEPRKEGQTP"
     gene            complement(4751..5599)
                     /gene="panC"
                     /locus_tag="PLY56_01140"
     CDS             complement(4751..5599)
                     /gene="panC"
                     /locus_tag="PLY56_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOJ20114.1"
                     /translation="MSVTERIEELRSLLAPARREGRSIGFVPTMGYLHAGHLTLMQRA
                     REENDVVVASIFVNPLQFGPKEDYQRYPRDLARDAELAREAGVDFIFAPSVEEMYPQE
                     MLAQVDVARITDRLEGAARPGHFRGVATVVAKLFNIVQPDRAYFGQKDYQQLLVIRRM
                     VRDLSYPIEVVAVPTVREEDGLAMSSRNSYLNPQERQAATVLIRALRLGRERIESGER
                     NAQRVRTAMQELIAQEPLARLEYLSIADPETLREQETITAPAFLSLAVFIGKTRLIDN
                     LLIETL"
     gene            complement(5577..6446)
                     /gene="panB"
                     /locus_tag="PLY56_01145"
     CDS             complement(5577..6446)
                     /gene="panB"
                     /locus_tag="PLY56_01145"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOJ20115.1"
                     /translation="MSATEQRPRVTVPRVVEMKARGEKVVMVTAYDTPTARLADEAGV
                     DLILVGDSLAMVALGYESTLPVTMEEMLHHTRAVARGARRALVVGDLPFLSYQASADD
                     AILNAGRMLKEGGARAVKLEGGEAVAPLVHRMVSSGIPVVGHVGLMPQSVHQMGGFRV
                     QGKTAEAARQVLRDAVALQDAGAFAVVLEAVPAPLAEAITARLSIPTIGIGAGAGCDG
                     QVQVVTDLLGLSERLPRHARPFLDLSRLIREAFEAYAAEVRAGSFPGPENATTMSDEE
                     LRKALADERDGTD"
     gene            complement(6468..7592)
                     /locus_tag="PLY56_01150"
     CDS             complement(6468..7592)
                     /locus_tag="PLY56_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ20116.1"
                     /translation="MPSERTHYEILGVGPGATSEEIRQRYRLLAKRYHPDVASASDAS
                     AAHRTFVEITAAYEVLIDRRKREAYDRELALRARRQSRDAGQASGARASAQAPPPRSG
                     PRRATPQPPPPRPEPPPDLGQMLHTARMDFAAGRISAAQAACHEILRHDRQNAGAHAL
                     LGDICRVQGRFEEALRRYRRAVELSQAARKGQSAERYTWGARALALARSMPPLALGGW
                     LLVLGLYLLPVVYPGSPVAEGGWMSGWTDNLLVGMIGSALLVGFLATSMRLVERPEGE
                     LRAPVWRRGRRSLSLGAVLLPASILCYPVVVLVALLLGVAGRRLPRPMIHLLGISCIL
                     LLLFAVAGPAGGETLLLGGNIIFPCMVLGCLAGGHFRDAW"
     gene            7794..8579
                     /locus_tag="PLY56_01155"
     CDS             7794..8579
                     /locus_tag="PLY56_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ20117.1"
                     /translation="MQSTRAIWVAVGVMAVALAVGTPAHAQVTLNGRQTNFAARVFTI
                     GNFLMVPLNETLTSLGAGPAVWDPDRGRARFTFQGSEVLIRAGSGYAIVDGQAVVMGV
                     PAVVRQNRLFIPLSFMREQFGVSVDVPAAVAGSEARARVLGFRGGPQTPNPGAIAIGG
                     TPILELSTTGDADTLNARAILVTERMTTGLQRAMQLDNGGFNPNRVTLRMVDGTPVIF
                     IADVPVISVTEEDAAAHQSTPRRLAFTWLRQIRSRLNSIFPAS"
     gene            complement(8699..>10329)
                     /locus_tag="PLY56_01160"
     CDS             complement(8699..>10329)
                     /locus_tag="PLY56_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HOJ20118.1"
                     /translation="PAALVAALPVSSTPLTWSLTCLLSEQALQRVLGARPAEAWGNGP
                     LLSRQEGRPHLEVSALQWLAWEQWGLAPEATARLLGCREAGLPADALPACSGWQLMRR
                     RWRRLGFSIACRQAASRGKPRLLLAARRLADLERRSLQSLSDLDLLDASVANAETALG
                     YLPEVIRAICRSASALFRLEETLARRMGPDYSPALLGEVLAREGSALGVRAAELLRRL
                     ARQAAEESQAREALEQIASSGDSDTGASEAWLARFAGTRAEPLLRRFLQRFGHHSLVD
                     MELSEPRWSEDCRYPLRVAASYLTADLPAMQGARPRARVAWAQAKRHLSLWEQYRCRR
                     LIGSLLHGVHLQEAAHALLARQVALERRLCLEAGSRLANRQALEAAEDVFLLNASELR
                     AFLLGEDPKGDMRAVVARRRRELSSEQGAGARFAEDQAGSASLAGLGISGGRATGRAR
                     ILRSPAEGWNLQPGEIIVASRIGMAWAPLLQRAGGVILEGGGLLSQAGLLARECGVPA
                     VSEISDAITRIAEGTRVVVDGTRGKVEILGRSGA"
BASE COUNT         1782 a         3700 c         3264 g         1583 t
ORIGIN      
        1 gccaagccgg cgcaggcagg cgcgctggat cgccttccgc atggtcgccc ccgcccagtg
       61 cgaggtgatc gcgccggcgg ctagcatcta ccccgccgcg aacccgggcg agcgccgctt
      121 cgggcgcccc gcacgcctca ccgtggaatg agcccgtcca gaccctggga ttccgacata
      181 gggaatccga agcctgggag gcagggctgg ggcggcctat actcccgggg ccacagaacg
      241 gcggggggcg gaggtgaagc cccgaaaggg ctttgccgga aagcagcgcg aggcttcgag
      301 ctccgcgcgt gcaggcggcc ttctctttat gtcggaatcc caggcccgga ccggcaacga
      361 tttatcgcct caccgctttc cccgcaaacg tgtgaggata cagccggcgg ccaacagcgc
      421 catcgagagt aggctgatcg tggcacccgc gcggaaggag ggcgaatcga aacgaaactc
      481 gagggtgtgc tcgccctcgg gcacggcaac gccctgcatc aggtaatttg cccgcagcag
      541 cggcgccggc tcgccgtcga gaagtaccct ccaccccggg tagtgccgct gattgtggat
      601 caccacgccc gtcccgcgca ttcggatgcg cgcccgcgcc cagtccgggc ctgcttccca
      661 ggcgagaacc tcgccgctga agttgcgaaa ctccggcgca ccctcgatag cactccagcg
      721 cgcctctagc agcgtgcgaa acgcctcgcg cccgccggca acgcttcgcg cctgatccgg
      781 gatccagatc tgcggccgtg cgtcctccac ggcatagacc cggaccggct gcttcgagtt
      841 gcctgtcttc acggccatct cctcgcgcag ccccggaatc gcaagcaggt agggcgtcac
      901 cacgtgggtg acgcccagca gcgccagggt acgcgggaag ctctccagga tcgtggaggt
      961 ccggcccagc gtgttccggt catcgcagag gagcgcgtag gtcgcggtgc cgcggcgcgt
     1021 gtcgaggccg ccgaagaagt tgaagcagct caggccgtag aaggcgccgg tgttgggcgc
     1081 caggacctcc ttctgctcca ggtagggccc caggtcgcct ttccagccgc gcgcgcgccg
     1141 cgtcgcttcc agctgcacgc cggcatagcc gaaggtgtag tagcgggccg atggggacgc
     1201 gaccgtatgg aggagacgcg cgctcttcgg gagcgtcagc caggcggcag catcagcgaa
     1261 gcggtggaac cgcgccgcgt agatccacag gtcaacgaag ctgatggcaa agagcattcc
     1321 cgccagcacc gcccaccgtc tccgggaatc ccggcccggc gccgccagca ggcgcgcgcg
     1381 cagttgatcc aggccatacg cggccagaag cgccagggag aggcaggcaa tcaccatgaa
     1441 ccgacttggg aagcggaaat agctcatccc gggaagcacg tgccacacaa cccggaagaa
     1501 aggcgtgtgc cggcccagca cgatgagcag cgagaagagc agcaagaaga cgaagaagcg
     1561 gatggggccc ttccgtcgta atccataagc ggccgcgtag agcgcgagga acaggggaat
     1621 cagcccgacg tagccgttgt cccagaaatg cccgcgctcc gaaaacccca tctcgccccc
     1681 ggtggccgga ttcccatgga cgtaaggagc cacgaatgtg agcagattcg cgggcgggaa
     1741 gctgaactct gtggcccttt caaaagagat gcccccggag cgccccgatt gccgcgtcaa
     1801 ctccacgcca ggcagccagt gcgccgccga gagccccagc gccaccgcca gcacgaccag
     1861 ggcaccgcca accccgttga ccaccttccc ggccggcccg ccgggggccc cccggtcctc
     1921 gccccacccc agctcccaga tcgccagaaa cgcggcggcg agcgcagagt agtagacgat
     1981 ttgcgggtag cctgtggtga tctggatggc cagcaccacc ccggcaccga tccagctcgc
     2041 gggcgtcgcc gcgcgcacac cccgccggac gcacagcagc agcaggggga tccacgccgc
     2101 cgcctgcgca ctgtgcagcg tcgccacgtg caacacgaag aagccggacc aggcgtaggc
     2161 gagccccccc agcaacgagg ccgcgcgcga gcgccccagg tcgcggcaga gccaatactg
     2221 gcacgcggca gcggtgagca cggcgaggat gacgttgacg ttgtaggcgg tcacgggcgg
     2281 cagcagccgg tagagaagga ggtggggcgg gtagagaacc cccgcctcgc cgagcgccag
     2341 cagcggatag ccgccgccga tgcggtcggt ccagacgggg agccggccct cgcgcagtgc
     2401 ctcggccagg agaaaggcaa acggcatatt gacatgccac tggtcgcaga ggggagtgag
     2461 cgatgtgatc agcacctgcc ctgcgagaag cggcaggaag cgcgcggtgc caattgtcaa
     2521 tagggccacc gcaatcacca cgcgaaggcg ccgccgttcg gggggctttc tcaccccctg
     2581 atcctcatca gttgttccgt aagcaggcat atgctataat cggcgtatgc agaccgcgaa
     2641 ggatgccgtc acacagccgc caagtcttcc gcagaccctt cccgagggcg cacgacagca
     2701 gagcttgcca gggcgctacg tcccctgggc agggctgctc tttctctgcg ccttcgcctt
     2761 ctatatcgcc accctggccc ctgggctcat gcccggcgac accggcgata tggtcatcgc
     2821 cgccgcatcc ctgcgcgagg cgcatccccc gggctaccct ctctatgcca tgattggcaa
     2881 gctgtttgtc gccttgccca tcggcgatat cccctatcgc gccaacctga tggccgcctt
     2941 cctcggcggt ctctccgtcg cgcttgtctt cctcgccctc ctccttctca cgcggcgccc
     3001 cctggcatcc cttgccggcg cgctgctctt tgccttcaac tacctcttct ggacctactt
     3061 caccctggcg gagaccttct gcctcaacaa cttcaccctg gcgctcaccg ccttcctcgc
     3121 cctcctgtgg cgagagcgcc accgccagga gcccggcggg agagcatccc ggcgtctcct
     3181 ggtcctcctc gccttcgtcg cgggggtctc gatgggcaac caccacacga tggtgctcac
     3241 ctatcctggg ttgttcctct ttgtcgccgt ggtcgaccgg agcgttctgc gccctgcccg
     3301 gctggcggcg ctggcgggat ggtttgcctt cggcctggcg cttgtctatg cctacctgcc
     3361 catcgcggcg ctggtgcacc acgaccacca ctgggaagac tcgagcacgc tctcgggctt
     3421 gctgcggctt ttcctccgcg ccaagtatgg caccttccag ctggcgcctg gtgacggcaa
     3481 gccggtcacg ccacccgagc aggcggccag ttacttcggc gcgctggcca cacagtacct
     3541 ctggcctggg tttgtgcttg ccctcgtcgg cctggccgcg ctgtggaagc gagaccgcgc
     3601 tgccctgctc cactcgggcg ggggattcct cttcggcgtc ctcttcattc tgaccgccaa
     3661 catcgatccc cggcccaccg cttacgacgc ggcaacgctc gagcgcttct accagcttcc
     3721 gaatttcttc ttcctcctgt ggaccggcgc cggactgggt gtcgccgagc gctggctgga
     3781 gggcatctgc gcggatatct cttcccgcgc ccccattggc aaagccatcc cggccatcct
     3841 gctcctcgcg ctaccggccg ccctccttgc ggtcaactac ggcaaggcga atcttcgcgg
     3901 caacttgctc tacccgcgct tcatgaccaa catcttcgat tccctgccga aaaacgccgt
     3961 gttctttgtc tggagcgata ccgtgggcat gggcgccgac tacctcacag atattgagaa
     4021 gcgccggcca gacgtggcgg tgatcaaggt gggcatcctg ggcgcggagc cgtaccgatc
     4081 cacggtacgc agaaagtatc cccaccttcg ctggcccgat ctcgcccccg acgcgccctt
     4141 tagcatgggt cgcttcatcc agcacaacat tgaccgctac cgcatcttcc tggatggcct
     4201 cccgatgaag ctcaccttcc cggtcatccg ctacgggctg atctacgagg tccgccccca
     4261 aggcagcgtg ccaagcgcag ccgaggtgct ggatttgaac gaagagctct ggaagcggtt
     4321 tgatctgcgc caggtggata cgagcctcta ccccgccaac tcgatgtgct actccgtcgt
     4381 ggtcttctac tacctctatc accgcttcac tatggcgagt gagtgcaact acttcgggcg
     4441 gtatgatgcc gcgctgcgcc acctggaagc ctgctctcag atggacccga aaggctacgt
     4501 ggcgcgctcc gccccctggc agcggcaaaa ggcggtcgcc cacatcggtc tcaaccagct
     4561 ggatcaggcc gtgacgcacc tcgatctcgc ccgcaaggag gacccatcgg acgccgagac
     4621 ctaccggctc ctctcactgg cctaccaaaa gaagggcgat caggcacaag ccgactatta
     4681 cctcgacgag tatgagatcc ggcgccgcgc cgaggagcaa gagccccgca aggagggcca
     4741 gacgccgtga tcacaacgtc tcgatcagca gattgtcgat cagccgggtc ttgccaatga
     4801 agacggccag ggagagaaac gccggcgcgg tgatggtctc ctgctcgcga agcgtctcag
     4861 ggtcggcaat gctcaggtac tccaggcggg ctagcggctc ctgggcaatc agctcttgca
     4921 tcgcggtacg cacacgctgc gcgttgcgct cgccgctctc gatccgctcc cggcctagcc
     4981 gcagggcgcg gatgagaacc gtcgccgcct ggcgctcttg cgggttgaga taggagttgc
     5041 ggctgctcat cgccaggccg tcctcttcgc gcaccgtggg cacggccaca acctcaatcg
     5101 gatagctgag gtcgcgaacc atgcgccgga tcacgaggag ctgctggtaa tctttctgcc
     5161 cgaagtaagc gcggtccggc tgcacaatgt tgaagagctt ggcgacgaca gtggccacgc
     5221 cccggaagtg cccggggcgc gcggctccct ccaggcgatc ggtgatccgc gctacgtcga
     5281 cctgggccaa catctcctgc gggtacatct cctcgaccga cggggcaaag atgaaatcaa
     5341 cgcccgcttc cctggcaagt tccgcatcgc gcgccagatc gcgaggataa cgctggtagt
     5401 cctccttcgg tccgaactgc agcggattca cgaagatgct cgccacgacg acgtcgttct
     5461 cctcgcgcgc gcgctgcatc aaggtgaggt gcccggcgtg caggtagccc atcgtgggca
     5521 caaagccgat gctccggccc tcacgccgtg ccggagccag gagtgacctc agctcctcaa
     5581 tccgttccgt cacgctcatc tgccaatgcc tttcgcaact cctcatcgct catggtggtg
     5641 gcattctcag ggcccgggaa actccccgcg cgcacctcgg cggcgtacgc ctcaaacgcc
     5701 tcgcggatca gtctgctcag atccaggaac ggacgcgcgt gcctgggcaa ccgctcggaa
     5761 agccccagga gatccgtcac cacctgtacc tgcccatcac acccggcgcc cgcgccaatc
     5821 ccaatcgtcg ggatggagag acgggcggtg atcgcctcgg ccaggggagc cggcacggct
     5881 tccagaacca ccgcgaaagc acccgcgtcc tggagcgcca ccgcatcgcg gagcacctgg
     5941 cgcgctgcct ccgccgtctt cccctgcacc ctgaagccgc ccatctgatg caccgattgc
     6001 ggcatcaaac ccacgtgccc gacgacaggt atcccggaac tgaccatccg gtggaccagg
     6061 ggcgcgaccg cctctccccc ctccagcttg acggcgcgcg ctcccccctc tttcagcatc
     6121 cggccagcat tcaagatcgc gtcgtccgcg ctcgcctggt acgagagaaa cgggagatcc
     6181 cccaccacca acgcgcgtcg tgctccacgg gcaaccgccc gggtgtgatg cagcatctcc
     6241 tccatcgtca ccggtagcgt gctctcatag cccagggcca ccatcgccag ggagtcgcct
     6301 accaggatca ggtcaacacc ggcctcgtcc gcgagccgcg ccgtcggagt gtcgtaggcg
     6361 gtcaccatca ccaccttctc accccgcgcc ttcatctcca ccacgcgggg caccgtcaca
     6421 cgcggtcgct gttccgtcgc gctcatcaaa actacgattc ccttcagcta ccaggcatcc
     6481 cggaagtgcc cgccagccag gcaccccagg accatgcacg ggaagatgat gttccctcct
     6541 agcagcagcg tctctcctcc ggccggtcca gccacggcaa agaggagaag caagatacac
     6601 gatatcccca acagatgtat catgggccga ggaagccttc ttcctgcaac cccaaggagg
     6661 agagccacca gcaccaccac gggatagcag aggatgctgg ccggcaggag caccgctccc
     6721 agagaaagcg accgccggcc tcgtcgccac accggcgcgc gcagctcgcc ctccgggcgc
     6781 tccacgaggc gcatcgaggt cgccaggaaa ccaaccagaa gcgccgagcc gatcatcccg
     6841 accaggaggt tgtcggtcca cccgctcatc catccgccct cggccacggg cgagccgggg
     6901 tagaccacag gaagcaggta gagccccagc accagaagcc atcccccgag ggcgagcggg
     6961 ggcatggagc gcgccagggc gagcgcccgt gctccccagg tgtagcgctc cgcgctttgg
     7021 cccttccgcg cggcctggct cagctctact gcccggcggt accggcgaag tgcctcctcg
     7081 aaacgcccct gcacccggca gatgtcaccg agcaaggcat gcgcgcccgc gttctggcgg
     7141 tcgtggcgca ggatctcgtg acacgcggcc tgcgccgcgc tgatgcgccc cgccgcgaaa
     7201 tccatgcgcg ccgtgtggag catctgcccc aggtctggag gcggctctgg acgagggggc
     7261 ggtggttgcg gcgtagcacg gcgcgggccg gagcgtggcg gcggcgcctg agcggaggcg
     7321 cgcgcgccag aagcctggcc cgcatcccga gactgccgac gggctcgcag cgccaattcg
     7381 cgatcatacg cctcgcgctt ccgccggtcg atcaggacct cgtaagcggc cgttatctcc
     7441 acgaaggtcc ggtgcgcggc gctggcatca gaggcactgg cgacgtccgg gtgataccgc
     7501 ttcgccagga gtcggtagcg ctgccggatc tcctctgaag tcgcgcctgg ccccactcca
     7561 aggatctcgt aatgggtgcg ctcagatggc atcccggcct ccacctccca agaggccaac
     7621 tcattatagc acggcagaac cgttcacgaa aggggatcgc gcgggcggtc ctcacgacca
     7681 tacgcctgcc cgccgcgttt gcccagagcg gcacccgggt aaatgccgtc cggaccttgc
     7741 gggatgccga gaccccggct cacccggcga gagtctgaaa aggggtgaca cgaatgcaaa
     7801 gcacgagagc gatatgggtg gccgtcgggg tgatggccgt ggcgctggct gtcggcacgc
     7861 cggcgcatgc gcaggtcacc ctcaacggcc ggcagaccaa cttcgcggcc agagtgttta
     7921 ccataggcaa cttcctgatg gtgccgctga atgagacgtt gacatcgctg ggagctggac
     7981 ccgcggtttg ggatccggat cgaggccgag ctcgcttcac cttccagggc tccgaggtgc
     8041 tcatccgcgc cgggagcggc tatgccattg tcgatggcca ggccgtggtg atgggcgtgc
     8101 ccgctgtcgt ccgccagaac cggcttttca tcccgctcag cttcatgcgc gagcagttcg
     8161 gcgtgtcggt ggatgtgccc gcggcggttg caggatctga ggcacgtgcg cgcgtgctgg
     8221 ggttccgcgg tggcccgcaa accccgaacc cgggcgccat cgccatcggc ggcacgccga
     8281 tcctcgaatt gagcacgaca ggggatgccg atacgctgaa tgcacgcgcg atcctggtca
     8341 ccgagcgcat gacaaccggc ctccagcggg cgatgcagct cgacaacggc ggcttcaacc
     8401 ccaaccgggt cactctgcgc atggtcgatg gaacgccggt catcttcatc gcggatgttc
     8461 cggtcatcag cgtgacagag gaggacgcgg cggcgcatca gagcacgccc aggcggcttg
     8521 cctttacctg gttgcgccag attcgcagcc gcctcaactc gatcttcccc gcctcttgag
     8581 gcgggcacag cctgaaagac agcactgagc gacaggcgca ggcgtcagga tgccggcgat
     8641 agcatcctga cgcccgcgcg ctccggccgg ctcgcatccc atctcgctgg tcgggggttc
     8701 aggcaccgga ccgccccagg atctcgacct tgccccgggt gccatccacc acgacgcgcg
     8761 tgccctcggc gatgcgcgtg atcgcatccg agatttcgga gaccgcggga acaccgcact
     8821 cacgggccag gagcccggct tgcgagagca gcccccctcc ctccagaatc acgccccccg
     8881 cccgctggag gaggggcgcc cacgccattc cgatgcgcga cgccacgatg atctctcccg
     8941 gctgcagatt ccagccctcc gccgggctgc gcaggatgcg cgcgcgtccc gtcgcccgtc
     9001 caccggatat ccccaggccg gcgagcgagg cactgcctgc ctggtcctcg gcgaagcggg
     9061 ccccagcgcc ctgttcggac gagagctcac gccggcgcct ggccaccacg gcgcgcatgt
     9121 ctcccttcgg atcttcaccc aacaggaagg cgcgcaattc ggaagcattc aggaggaaga
     9181 catcttcggc ggcctcgagg gcctggcggt tcgcgaggcg cgatccggcc tccaggcaga
     9241 ggcggcgttc cagggctact tgccgggcca gaagagcgtg cgcggcctcc tgcaggtgca
     9301 cgccatgcag gagggagccg atcagccgcc ggcaccgata ctgctcccac aggctcaggt
     9361 gtcgcttcgc ctgggcccag gccacgcggg cgcgcgggcg cgctccctgc atggccggca
     9421 ggtctgccgt caggtaactc gccgcgaccc gcagcgggta acggcaatcc tccgaccaac
     9481 gcggctccga gagctccata tcgacgagcg aatgatgccc gaagcgttgg agaaaccgcc
     9541 ggagaagcgg ctccgcgcgt gtgccggcaa accgtgcaag ccacgcctcc gaggctcccg
     9601 tgtcgctatc gccggacgat gcgatctgct cgagggcctc gcgcgcctga gactcttcgg
     9661 ccgcctgcct ggccagccgg cgcaacagct cggcggcgcg caccccgagg gcactcccct
     9721 cgcgggcaag cacctctccc aggagcgcag gcgagtagtc cggccccatc cgccgggcga
     9781 gtgtctcctc caggcgaaag agtgcggatg ccgaccgaca gatcgcccgg atcacctcgg
     9841 gcaggtagcc gagggccgtc tcggcgttgg caacactggc atccagcagg tccagatcgc
     9901 taaggctctg cagggagcgg cgctccagat ctgccaaccg gcgtgccgcg agaaggagac
     9961 gcggcttccc cctgctcgcg gcctgccggc aggcgattga gaagccgagc cggcgccacc
    10021 gccggcgcat cagctgccac ccgctgcagg ccggcagtgc gtccgctggc aacccggcct
    10081 cgcggcaacc caggaggcgc gccgtcgctt ccggggcaag tccccactgc tcccatgcga
    10141 gccattgcag tgccgagacc tccagatgcg gccggccctc ctggcggctg aggaggggcc
    10201 cgttacccca tgcctccgcc ggccgggcgc ctaacacgcg ctgcagggcc tgctccgaga
    10261 ggaggcaggt gagcgaccag gtgagcggag tggatgagac cggtagcgcc gcgacaagag
    10321 cggcaggcg
//
LOCUS       DAOSCV010000023        10178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4331_length_10178_cov_3.279364, whole genome shotgun sequence.
ACCESSION   DAOSCV010000023 DAOSCV010000000
VERSION     DAOSCV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4331_length_10178_cov_3.279364"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1884
                     /locus_tag="PLY56_01165"
     CDS             <1..1884
                     /locus_tag="PLY56_01165"
                     /inference="COORDINATES: protein motif:HMM:NF027224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BNR-4 repeat-containing protein"
                     /protein_id="HOJ20119.1"
                     /translation="AKGTALHTFDLAKPAADQSFSVPAEAGARDGLWHLDLKAMDVRI
                     RVSNVTHWTFDAAAYFDPAPLRWMLLPYNEARYLKPGQSAELQYTLRNRTDRPTAFRV
                     EATGRDGLACRVIEPASPVALEKGEARAIRVAVRVSATVKPGSVLEGVVSATASHDAE
                     VAQSAGIEVRIGDSPVSRPLKLPVVHERYRHENALFGYAPDYVPNEVYFDRQNRPVIR
                     QRTESAYLTNALTLLEGERWVERSFVPALQKAYPGYRGIYFGGGFLGAKVAFDGENGA
                     YTIVSVMTADRKRQSVLLYTPDGGRTYTTHELPGNQFDIEQFTGHNALSIPPPVLAYR
                     QTASRPEPFASVNDLLLYLPKKVNGKLVLGEPVLVSNRCLGSCQHSGGPASTATRDGK
                     THIVWGEVTDEKVPGVPTYVATYDHATGKLSEKVLLAYAPPINDVHNVPAICMDSQGY
                     LHVVAGAHGEPFQYLRSLRPNDSSTWTKPVEVLSAGYITDKTDADGSGRQTYVSLVCG
                     PDDTLHIAFRQWRRGVDPYHGGEIYAALSVQSKPKDGPWGPARPLVVPPVPGYSIYYH
                     KLTIDRRGWLYLSYNHWTSDESYQNEFPERHHHRAVLGSKDGGKSWKLAETRDFLEGI
                     ARR"
     gene            complement(1943..2016)
                     /locus_tag="PLY56_01170"
     tRNA            complement(1943..2016)
                     /locus_tag="PLY56_01170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1982..1984),aa:Gln,seq:ctg)
     gene            complement(2210..3196)
                     /locus_tag="PLY56_01175"
     CDS             complement(2210..3196)
                     /locus_tag="PLY56_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20120.1"
                     /translation="MLKVGIIGCGGIAHAHADGWLAAGDLAKVTAVCDVDAGNRAKMA
                     EKVGGAEGFADWSEMLAKADLDAVDICLPHHLHKAAIVAAADAGKHVLCEKPLCITLA
                     EAAEITAAKERNGITIACAHNQIFTPCVQRARQLLDEGVFGRVLSVKTADCFRISRDK
                     ETWGWRASLATAGGGELIDTGYHPSYLLLYLAGSRPKQVTAMIQNFDQPLLEGEDSAN
                     VLVRFENGAMGQIFTSWSWEWPGGFWKFHVIGEKGQMWGSGQQFFRKMLGQDVEQEEL
                     PPMNGFHAETRHFAECLATGARPVQSEEDGIDVLRVILGAYEASREGRVVEL"
     gene            3524..4663
                     /locus_tag="PLY56_01180"
     CDS             3524..4663
                     /locus_tag="PLY56_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ20121.1"
                     /translation="MIRNERGSTFGYFLAALLGALLGGMLVVTVLNKEQKQTEAAPPL
                     PINSTASLPRSTDTGTVTEAVKRIGPAVVSIDTRVVRPRSDIPEFFREFFGDESAPMP
                     QEGKGSGVIIDARRGYVLTNAHVIKDAREIKVTLPDKRQFTGKVVGQDVQSDLAVVRI
                     PANNLPEAKLSKAAPPPIGSWVVAIGNPFGFANTVTVGVVSATERDISPQGGPTLQRL
                     IQTDAAINPGNSGGPLVNLDGEVVGINTAILSVAQGIGFAISSQHAHEVAQQLIERGK
                     VVRPWIGVSYMPVTQEFKEYFDLPTTEGVVVRDVIEGSPAARAGVRRGDLIVEIDRKQ
                     IKEADDLKNEILKRKVGDTVSLLIYRGKESKVIKLRTAEMPQNLR"
     gene            4660..5784
                     /locus_tag="PLY56_01185"
     CDS             4660..5784
                     /locus_tag="PLY56_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20122.1"
                     /translation="MIRAAKRGARGSAGRPASRQIDQACQAYVRHAPSRLGALVALLV
                     FAAGALGAPARAQSKEESVAQCVAALRARPEDDEAKRLLRRAFTEVYPSFLPADILTF
                     LPLPHQVCEDRATESAQPQRTFFTTGLAFPDASRRQDPEKRQAFNRVLHAYVQEEGGE
                     WRLAFRVLYDAHQEGAEELAPRAMRELLRVRAVTLAHIGDPRRALSAPVDLWLLGNGD
                     GGAEQSGPNLYLHGTRNTRAPLELLRQIAHELGHLIFPAIGPLDEPETWGNGFLGEVL
                     ILRWLARAPAPKESAVPAEALAAYVRQEVEPLARAFAVAGWAGFAGEERSAGSLRLLL
                     GMVLHLEDTQGTKALSELMRSLNGTTVPHWIDAARPLLTP"
     gene            complement(5804..6811)
                     /locus_tag="PLY56_01190"
     CDS             complement(5804..6811)
                     /locus_tag="PLY56_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HOJ20123.1"
                     /translation="MEVGKLPAELLARLLSRHPVTDPRVLVGPAPGEDAAAIDMGDRV
                     LVAKTDPITFATDAIGWYAVNVNANDIATMGAEPRWFLATVLLPEGAEERLAEEIFEQ
                     IATACEELGIALVGGHTEITYGLSRPVIAGCLLGEAERERLLPSRGARPGDILVLTKG
                     IAVEGTAILAREARQDLKRAGVASDLIDRAAEFLHRPGISVVRDARLAMNTGGVHAMH
                     DPTEGGLITGLREIATAAGTGIRVDAGAIPVLPETRVICDALKLDPLGLIASGALLAA
                     VAPDRAPMLLDAYHNAGIVAAAIGELTHPNDGMWLEREGERLPLPLFDRDEIARYFSP
                     Q"
     gene            complement(6821..9136)
                     /locus_tag="PLY56_01195"
     CDS             complement(6821..9136)
                     /locus_tag="PLY56_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ20124.1"
                     /translation="MSRTIRLEYGKALLEATCLVETGAPGSGEIQTWEAWEFAPPETR
                     EGTLACLGKGRFTTETAEKGDLRLTRETFRPEDGPARLAVRLLLTNLGPDDLEVATLS
                     PLVVGEEGARLGAAPAGEWVFLRQPRHKNDMPAAFRLGDETPGVWDGVRGTPETGGVP
                     RGAGETGFPTRFVGSEMMLLRAPEAALFLGFFPLDRQLVQGVVGLTPDRRRLAGIRLD
                     CHCDGQLLAPGKTLVSQWVLIDLDPDAFAAIERYTELLNDVHPGAREREKARKRPPTV
                     WCSWYYYGDGCAQWEAEANLDALRKRPLPIDVIQIDECWDQEWGDWYPNIRWPDLPGF
                     ARAIREAGYQPGIWTCPFLADIRSTVRYHHPEWLLHRRNGEPVIFPMGTQRSFVLDPT
                     HPEVLVFLEELYHRLTHEWGFTYHKLDFTRAIGEPDVLFYDRTKNRAEAYRAAFEAIR
                     RGIGPDAYLNVCGGFYGPLVGLADAQRTGSDVRSEWPEPPPGQEGEGYGPFTIKQNTL
                     RFWMNRLWDNDPDALMVRRRAEAYRGEVLSLGRMNDDEALTSALNQYLGGGLVCFTEN
                     LAEVDDDRLLLLRHCAPSIGEAAIPRDALRGVRFPALFDTRVTPAAPGLSSWHTLSIV
                     NWHSEARSYRVALDEEILGHFATEHPAFMASAFRGEWARVVRPGEVLEIGPIRAHACE
                     VVKIQPLPSDGPCLVWTDGHFSMGGTEVIAWEPYAGGVRLQVKWLWPTPLTLRVAPAP
                     GKRFASARADGLAEIRLEGPLASRWLDLPYA"
     gene            9408..>10178
                     /locus_tag="PLY56_01200"
     CDS             9408..>10178
                     /locus_tag="PLY56_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ20125.1"
                     /translation="MMRETTDFDVVVIGGGHAGCEAALAAARLGCRTLVLTLDLERIG
                     HTPCNCSIGGPAKAHVVREIDALGGEMALAADAATTHRRLLNTSKGPAVQALRVQVDK
                     GLYSRRMRAALEAQPRLTVVCDRATDLLAEDGQIAGVRTASGTTYTAKAVVITTGTFL
                     RGKCHIGERSWDAGRAGEPPAMELSESLRALGFTLVRFKTGTTARVDKKSIDLERTIV
                     QPSEPESPPFSYLTPHEPREELLPCWLTFTTEETAAIIR"
BASE COUNT         1739 a         3425 c         3418 g         1596 t
ORIGIN      
        1 gcgaagggca ccgcactcca caccttcgac ctggcgaagc cagccgcgga ccagagcttc
       61 tccgtgcccg ccgaggcggg cgcgcgcgac gggctgtggc acctcgatct gaaggcgatg
      121 gacgtgagga tccgcgtctc caacgtgacg cattggacct tcgacgccgc cgcctatttc
      181 gacccggccc ccctgcgctg gatgctcctc ccgtacaacg aggcgcgcta tctgaagccg
      241 ggccagagcg ccgagctcca gtacacgctg cgcaaccgca cggaccggcc cacagcgttc
      301 cgggtggagg ccacgggccg cgacggcctg gcctgccgcg tgatcgagcc ggcctcgcct
      361 gtcgcgctgg agaaaggcga ggcacgcgcg attcgcgtcg ccgttcgggt gtcggccacg
      421 gtgaagcctg gctccgtgct cgaaggagtt gtgagcgcca cggcctcgca cgacgcggag
      481 gtggcgcaga gcgccgggat cgaagtgcgc attggcgatt cgcccgtctc gcgcccgctg
      541 aagctgccgg tcgtccatga acgctaccgg catgagaacg cgctgttcgg ctacgcgccc
      601 gactacgtgc cgaacgaggt ctatttcgac cggcagaacc gaccggtgat ccgccagcgc
      661 acggagagtg cctacctgac gaacgcgctc accctgctgg agggggagcg atgggtggag
      721 cgctccttcg tgccggcgct gcagaaagcc tacccgggct accgaggaat ctacttcggc
      781 gggggcttcc tgggcgcgaa ggtcgctttt gatggcgaga acggcgccta cacgattgtg
      841 agcgtgatga ccgccgaccg caagcgccag agcgtgcttc tctacacgcc ggacgggggg
      901 cgcacctata cgacgcacga gctgccgggg aaccagttcg atatcgagca gttcacgggg
      961 cacaacgccc tctccatccc cccgccggtg cttgcctatc ggcaaacggc ctcgcggccc
     1021 gaaccgttcg ccagcgtgaa cgacctgctc ctctacctgc ccaagaaggt gaacgggaag
     1081 ctcgttctgg gcgagccggt gctcgtctca aaccggtgcc tcggctcgtg ccagcactcg
     1141 ggcgggccgg cctcgacggc gacgcgcgac ggcaagacgc acatcgtttg gggtgaagtg
     1201 accgatgaga aggtgccagg ggtgcccacc tacgtcgcca cgtatgatca cgccacgggg
     1261 aagctgagcg agaaggtcct cctcgcctac gcgcccccga tcaacgacgt gcataacgtg
     1321 ccggcgatct gcatggatag ccagggctac ctgcacgtgg tggccggcgc gcacggcgaa
     1381 ccattccagt atctccggtc gctgcggccc aacgactcca gcacctggac caaaccggtg
     1441 gaggtccttt ccgctggcta catcaccgac aagacagatg ccgatggatc cggccggcag
     1501 acctatgtct cgctggtgtg cggcccggac gatacactgc acatcgcctt ccggcaatgg
     1561 cggcgggggg tggatcccta ccatggcggg gagatctacg cggcgctctc ggtgcagagc
     1621 aagccgaagg atggaccgtg gggtcccgcc cggccgctcg ttgtcccgcc cgtgcccggc
     1681 tattcgattt actaccacaa gctgacgatt gaccggcgcg gctggctcta cctgtcgtac
     1741 aaccactgga cgagcgacga gagctaccag aacgagtttc cggagcggca tcaccaccgc
     1801 gcggtgctcg gctcgaagga tggcgggaag agctggaagc tggcggagac gcgagacttc
     1861 ctggagggaa tcgcgcgccg gtagatcagg cacgctcgca gatatgacaa agggccgcgc
     1921 tctaaagagc gcggcccttt cgtggctgcc cgggatggat tcgaaccacc gtcgactgat
     1981 ccagagtcag ttgtcctgcc actagacgac cgggcagtat cccatcctgt cgtgcctatt
     2041 ataccaccgg cacgcacacg tgtcaacacc ctgttcgcgc aatcgcaccc gacccgagag
     2101 gcgcgggcca accgaccggt aaggtttcac gcatgcgagg caaccggcgc gggggttcgt
     2161 gcccgcgccg gtctccgtct ccgcgaaacg aggcggttgc ccccggaggc tacagctcta
     2221 cgacgcggcc ctcgcggctc gcctcgtagg cacccaggat aacgcgcaga acgtcgatcc
     2281 catcctcctc gctctgcaca gggcgcgcgc cagtggcgag acattcggcg aagtggcgcg
     2341 tctcggcatg gaagccgttc attgggggca actcttcctg ctccacatcc tggcccagca
     2401 tcttccggaa aaactgctgc ccggagcccc acatctgacc cttctcgccg atgacgtgga
     2461 acttccagaa gccgcccggc cactcccacg accaggaagt gaagatctgg cccatcgcgc
     2521 cattctcaaa gcgcaccagg acgttggcgg aatcctcgcc ctcgagcagg ggctgatcga
     2581 agttctggat catcgctgtc acctgcttcg gacggcttcc cgcgaggtag aggagcaggt
     2641 aggaggggtg gtagccggtg tcgatcagct ctccgccgcc cgccgtcgcg agggaggcgc
     2701 gccagcccca cgtctccttg tctcgggaga tgcggaagca atcggccgtc ttcaccgaga
     2761 ggacgcgccc gaagacgccc tcatccagca gctgccgggc gcgctggacg cacggcgtga
     2821 agatctgatt gtgggcgcag gcgatggtga tcccgttccg ttccttcgcc gcggtgatct
     2881 cggcggcctc cgccagggtg atgcagagcg gcttctcgca caggacgtgc ttgcctgcat
     2941 ccgcggcagc cacgatagcc gccttgtgaa gatgatgcgg caggcagata tccacggcgt
     3001 cgaggtccgc ttttgccagc atctcgctcc aatcagcgaa tccctcggca ccgcccacct
     3061 tctccgccat cttcgcccgg tttccagcgt ccacgtcgca caccgcggtt accttcgcga
     3121 gatcgcctgc tgccagccat ccatctgcgt gcgcgtgagc aatcccgcca cacccaatga
     3181 tcccaacctt cagcatggct cttccctctc tctccgagcg agtgcccgtg cacccgccgt
     3241 cacatttcgg tgcgcccaag atggctcctg ctgttgccgg cgcagaacag cgcccgcgcc
     3301 caccaggccc tcgcccgggc gctgtggcgg cgggagcagg acacgctgtt ggcctcatcg
     3361 aacagggatc gcgggggtgt cgcgggtcga acgcccatcc cgggccggcg atggaaccct
     3421 ggagccatgg agagcgtctg gataccagag accggcgcgg gcattttggc acctcatcgg
     3481 ccatgctgtt tgcgacgccc ggcctaacgc ggagggactc ttagtgatac ggaacgaacg
     3541 cggctccact ttcggctatt ttctggcggc cctgctcggt gcgctcctgg gcggcatgct
     3601 ggtggtgaca gtgctcaaca aggagcaaaa gcagaccgaa gcggccccgc cactgccgat
     3661 caactcgacg gcatcgctgc ctcgctccac cgataccggc accgtcactg aagcggtaaa
     3721 gcgcattggc ccggcggtgg tcagtatcga cacgcgtgta gttcgccctc gctcggatat
     3781 tccagagttc ttccgcgagt ttttcggcga cgagagcgcg ccaatgccgc aggaggggaa
     3841 aggctcggga gtcatcatcg acgcgcgccg tggctacgtg ctgaccaacg cgcacgtcat
     3901 caaggacgca agggagatca aggtcacgct gcccgataag cgacagttca ccggcaaagt
     3961 ggtggggcaa gatgtgcaat cggacctcgc cgtggtccgc atcccagcca acaatctgcc
     4021 agaggcgaag ctctccaagg cggctcctcc gcccatcggt tcgtgggtgg tggcgattgg
     4081 caaccccttc ggcttcgcga acaccgtgac ggtgggcgtc gtcagcgcga ccgagcgcga
     4141 tatctcgccc cagggcgggc cgacgctgca gcggctgatt cagacggacg cggcgatcaa
     4201 cccgggcaat tcgggcgggc cgctagtcaa cctcgacggc gaggtggtgg ggatcaacac
     4261 ggcgatcctc tcggtggcgc agggaattgg gtttgccatc tcgtcgcaac acgcccacga
     4321 ggtggcccag cagctcatcg aacgtggcaa agtggtccgg ccctggattg gcgtctccta
     4381 catgccggtc acccaggagt tcaaagagta ctttgatctc cccaccacgg agggtgtggt
     4441 ggtccgcgac gtgatcgagg gcagcccggc ggccagagcc ggggttcgcc gcggagacct
     4501 gattgtcgag atcgaccgga agcagatcaa ggaggcggat gatctgaaga acgagatcct
     4561 gaagcggaag gtgggcgata ccgtcagcct actcatctac cgcggcaagg aatcgaaggt
     4621 catcaaactg cgcacggccg agatgccgca gaacttgcgg tgatacgcgc ggcaaagcgg
     4681 ggtgcgcgcg gcagcgctgg ccgtccggca tcccgccaga tagatcaggc atgccaggcg
     4741 tacgtccggc atgctccttc ccggctcggg gcactcgtcg ccctcctcgt tttcgcggcg
     4801 ggtgccctcg gggctccggc gcgcgctcag agcaaagagg agagcgtcgc gcagtgcgtt
     4861 gcggcgctgc gcgcccgccc ggaggatgac gaggcgaaac ggctcctgcg tcgcgccttc
     4921 accgaggtgt accccagctt tctcccggcc gatatcctga cgttcctccc gctcccgcac
     4981 caggtgtgcg aggatcgcgc caccgaatcc gcgcagccac agcgcacctt tttcactacg
     5041 ggcctcgcat tcccagacgc cagccggcgc caggacccgg aaaagcggca ggcattcaac
     5101 cgggtgctcc atgcctatgt gcaagaggag ggcggagagt ggcggcttgc ctttcgggtg
     5161 ctctacgatg cgcaccagga gggcgccgag gagctcgcgc cccgggcgat gcgtgagcta
     5221 ctgcgggtgc gcgccgtgac gctggcccac atcggcgacc cgcggcgtgc cctgtcggcg
     5281 ccggtagacc tgtggctgct cgggaacggc gatggcggcg cggagcagtc cggcccgaac
     5341 ctctacctgc acggcacgcg caacacgcgc gcgccactag agctactccg ccagattgcc
     5401 cacgaactgg ggcacctgat ctttccggcc atcgggcctc tggacgaacc cgagacgtgg
     5461 ggcaacggct tcctgggtga ggtcctcatc ctgcgctggc ttgcacgggc accggcgccc
     5521 aaagagagcg cggtgccggc ggaagcgctg gcggcttacg ttcggcagga ggtggagccg
     5581 ctggcgcgcg cgttcgcggt ggcgggatgg gccggcttcg cgggcgagga gagatcggca
     5641 gggagcctgc gcctgctcct gggcatggtg ctgcatctgg aagacacgca gggcacgaag
     5701 gcgctctccg aactgatgcg ctcgctcaac ggcactaccg tgccccattg gatcgacgcg
     5761 gcacggcccc tgctcacccc ctagcccgaa cagcaggtat cgcctactgt ggcgagaagt
     5821 agcgggcgat ctcgtcccga tcgaagagag gcagcggcaa ccgctctccc tctcgttcca
     5881 gccacatgcc gtcattcgga tgggtcagct caccgatggc ggcagccacg atgccggcgt
     5941 tgtgataggc atccaggagc atgggcgcgc ggtcgggggc gaccgccgcg aggagcgccc
     6001 cggaggcgat caggccgagg gggtctagct tcagagcgtc gcagataacc cgcgtctccg
     6061 ggagcaccgg gatggcgccg gcatcgacgc ggataccggt gccggcggcg gtggcgatct
     6121 cgcgcagtcc ggtgatcagg ccgccctctg tggggtcgtg catggcatga accccgccgg
     6181 tgttcatcgc caggcgcgcg tcacgcacga cgctgatccc gggccggtgc aggaactccg
     6241 ccgctcgatc tataagatcc gaggccacgc cggcgcgctt gagatcctgc ctcgcctcgc
     6301 gcgcgagaat ggcggtcccc tcgacagcga tccccttggt caacacgaga atatcgccgg
     6361 gccgggcgcc ccgagaggga aggaggcgct cgcgctccgc ctcgcccagg aggcagccgg
     6421 cgatcaccgg gcgcgacagc ccgtaagtga tctcggtatg cccgcccacc agcgcgatcc
     6481 ccagctcttc acacgcggta gcgatctgct cgaagatctc ctccgccagg cgctcctcgg
     6541 cgccttccgg caaaagcacg gtcgccagga accagcgcgg ctcggcgccc atcgtggcga
     6601 tgtcgttggc gttcacgttc accgcgtacc agccgatggc gtcggtcgcg aaggtgatcg
     6661 ggtcggtctt cgccaccagg acacggtcgc ccatgtcaat cgcggcggca tcctcgccgg
     6721 gcgccgggcc gacaagcacg cgcgggtcgg tcaccgggtg gcgcgacagc aggcgggcga
     6781 gtagctccgc gggcaactta cctacttcca tccaacactc ctatgcataa ggcagatcga
     6841 gccaacgcga agccaggggg ccctccaacc gaatctccgc caggccatcg gcgcgcgcgc
     6901 tggcgaagcg cttccctggg gccggcgcca ccctcagtgt gagcggtgtg ggccagagcc
     6961 atttcacctg cagccgcacc cctccggcat acggctccca tgcgatgacc tcggtgccac
     7021 ccatcgagaa gtggccatcc gtccagacca ggcaggggcc atctgacggg agaggctgga
     7081 tcttcaccac ctcgcaggcg tgcgcccgga ttggcccgat ctccagcacc tcgccgggcc
     7141 gtaccacgcg cgcccactcg ccgcggaagg cgctcgccat aaaagcggga tgctccgtgg
     7201 cgaagtggcc caggatctcc tcgtcgagcg ccacccggta ggagcgggct tcggagtgcc
     7261 agttgacgat gctgagcgtg tgccaggagg agagcccggg cgcggcggga gtgacgcgcg
     7321 tgtcgaagag cgccggaaag cggacgcccc gcagggcatc acgcgggatc gccgcctcgc
     7381 cgatggacgg cgcgcaatgg cgcaggagaa gcagccggtc gtcgtcgacc tccgccagat
     7441 tctcggtgaa gcagaccagg ccgccgccca agtattggtt cagggcgctc gtgagcgcct
     7501 cgtcatcgtt catccgtccg agcgagagga cctcaccgcg atacgcctcc gcccggcgcc
     7561 ggaccatcag cgcatccgga tcgttatccc acagccggtt catccagaag cggagggtgt
     7621 tctgcttgat ggtgaagggg ccatacccct ccccttcctg gcctggcggg ggttcgggcc
     7681 actcgctccg cacgtccgag ccggtgcgct gggcatcggc caggccaacg agcgggccgt
     7741 agaaaccgcc gcagacgttg agataggcgt ctgggccgat cccgcggcgg atcgcctcga
     7801 aggcggcgcg gtaggcctcc gcccggttct tcgtgcggtc gtagaagagg acgtctggct
     7861 ccccgatggc gcgagtaaaa tcgagcttgt ggtaagtgaa gccccactcg tgcgtgaggc
     7921 gatggtagag ctcctccagg aagacgagca cttccggatg ggtgggatcg agaacaaagc
     7981 tgcgctgcgt gcccatcggg aagatgacgg gctcgccatt ccgccggtgg agcagccact
     8041 ccgggtggtg atagcggacg gtggagcgga tatcggccag gaaggggcac gtccagatcc
     8101 ccggttgata gccggcctcc cgaatggccc gggcgaagcc ggggagatcg ggccagcgga
     8161 tgttggggta ccaatcgccc cactcctggt cccagcactc gtcaatctgg atgacatcga
     8221 tcgggagcgg acgcttccgg agcgcgtcga ggttggcctc ggcctcccac tgcgcgcagc
     8281 catcaccgta gtagtaccag ctgcaccaga ccgtaggcgg tcttttgcgc gccttttccc
     8341 gctcgcgcgc gcccggatga acgtcgttca ggagctccgt gtaacgctca atcgccgcga
     8401 aggcgtcggg atcgaggtca atgagcaccc actggctgac gagcgtcttg ccgggagcga
     8461 ggagctggcc gtcgcagtgg caatcaaggc ggattccagc caggcgccgg cggtcgggag
     8521 tgagccccac cacgccctga acaagctgcc ggtcgagcgg gaagaagccg agaaagaggg
     8581 ccgcttcggg ggcgcggagg agcatcatct cggagccgac gaagcgggtg ggaaagccgg
     8641 tttcccccgc gccgcgcggc actcccccgg tctctggtgt gccgcgtacg ccgtcccaca
     8701 ccccgggtgt ttcatcgccg aggcggaagg ccgctggcat atcgttctta tgccgcggct
     8761 ggcggaggaa gacccactcg cctgcgggcg cggcgcccag acgggcgcct tcctcgccca
     8821 cgaccagcgg tgagagcgtg gccacctcca ggtcatccgg ccccaggttg gtgaggagaa
     8881 ggcgcaccgc caggcgcgcc ggaccatcct cgggtcggaa cgtctcgcgc gtgaggcgaa
     8941 gatcaccctt ctccgcggtc tccgtggtaa agcggccctt gccgaggcac gcgagcgtgc
     9001 cttcgcgcgt ctcgggcggc gcaaactccc aggcttccca ggtttgaatc tccccgctcc
     9061 caggcgcgcc tgtctctacc aggcaagtgg cctccaggag ggccttcccg tactccagtc
     9121 ggatcgttct gctcacctcg cgccctttca catcgcgcca gcggcatggc cctcaccacc
     9181 agggcgtcga cggaagcggt ggggaatcga agcagccttc tgccccgggg gagggattcc
     9241 gcgggcgcat cggtctatag gtgtcagttc gcggcggaac attctgtacc cttgttcgga
     9301 aaagccctcc gccacggaag ggcacgccct gccccgggtt gtacctgaat cgttgcgaga
     9361 aaagcgggcc tttcgccggg cggcgggcgt acgctataat gggatcgatg atgcgagaga
     9421 caaccgattt tgacgtggtg gtaattggtg gcgggcacgc cgggtgcgag gcggcgctgg
     9481 cggcggcacg attgggttgc cggacgctgg tgctcactct cgatctggag cgaatcgggc
     9541 acacgccgtg caactgctcc atcggggggc cggccaaggc gcacgtggtc cgcgagatcg
     9601 acgccctggg cggcgagatg gcgctcgccg ccgatgccgc gacgacgcac cgacgcctgc
     9661 tgaacacgag caagggcccc gcggtccagg cgcttcgcgt gcaggtcgat aaggggctct
     9721 actcgcggcg gatgcgcgcc gcgctggagg cgcagcccag gctgacggtc gtttgcgacc
     9781 gggcgaccga tctcctcgcc gaagatggcc agatcgcggg cgtgcgcacc gcttccggca
     9841 ccacctacac ggcgaaggcg gtggtcatca cgacgggcac cttcctgcgc gggaaatgcc
     9901 acatcggcga gcggagctgg gacgccggac gggcaggtga gccgccggcg atggagctct
     9961 cggagtcgct gcgcgccctc gggtttacgc tggtgcgctt caagacaggc accacggcgc
    10021 gggtagataa gaagagcatc gatctggagc ggaccatcgt ccagccgtcc gagccggaat
    10081 cgcccccgtt ctcctacctg acgccgcacg agccacgcga ggaactgttg ccgtgctggc
    10141 tcaccttcac cacggaggag accgccgcga tcatccgg
//
LOCUS       DAOSCV010000024        10141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4353_length_10141_cov_3.931787, whole genome shotgun sequence.
ACCESSION   DAOSCV010000024 DAOSCV010000000
VERSION     DAOSCV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10141
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_10141_cov_3.931787"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLY56_01205"
     CDS             <1..702
                     /locus_tag="PLY56_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20126.1"
                     /translation="NEPDGCVPRRAAMPRNGRRNLPPEFHVGVPVVGPEGEVGTITRV
                     VVDPDTSAVIDIILRLRNGSESREVVLPADRVLSSDAHETRVDLTREDARRLPDLEEV
                     RLRKPEEGWEGLPGDTPDVVYFWMPRSAVEAFVPPSIVPEPNVEGTRNVPEGSVVVSA
                     DLDVVCGDEVIGHVDGVVTDAEGDHATHLIVERGLLRKDRHLVPVDRVREVTDSRVRL
                     ECDARGLAAYPRYQE"
     gene            706..1875
                     /locus_tag="PLY56_01210"
     CDS             706..1875
                     /locus_tag="PLY56_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOJ20127.1"
                     /translation="MPRDITPLTGLRENLILQEEKILQAWRALVPPEQTLLAESLADS
                     VRDLLLLMYECLSGIGTEAGTDTRHLAYQIAQQVLEEHDYQTLINVFRDALQEAVMDY
                     TALTSGDAARNLIAFFTIVTDAYWLAYSDGLRKAIRHQYRERLSNELRVAKRIQQRLL
                     PKVIPQIPGWEIAGRLVPALEVGGDYWSVKHYANDGVITCKLADVTGHGIGAAILVAA
                     VKFISGGFYRGAPSASWVMERTNHVLVVETPSDIMVTMVYTWIRPATGQITLVNAGHG
                     PVFACRDGQVRTLAPTGPALGLLESRYSEVELQFRPGDILFFCSDGIIEARSRDPAKA
                     REMFGTERLHEVVCGFSHLPAAEIADKVVQTAIDFSGTPNDDMSLVVIKRVSDDA"
     gene            2013..2972
                     /locus_tag="PLY56_01215"
     CDS             2013..2972
                     /locus_tag="PLY56_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20128.1"
                     /translation="MRKIRVGLIGAGGMANSVHYPSLAEFPDVEMAALCDLDEQKLQA
                     TAAKFGIERTYTDYRKMLDEVDPEAVYVLMPPHHLFDLAINILNQKRHLFIEKPPGVT
                     REQTRNMALTAERNGCLTMVGFNRRFIPLIREVRKRVDEHGPMLQCVATFYKNSVGAG
                     PYYGGAIDILTCDAVHAVDMLRYMGGEVAAVAGDVRALHATYDNSFNALVRFENGCTG
                     VLLTNWVVGKRVHTFEMHAKGFSAFIDGDTQATLYSGNNDPGEVLDSRAVAGSDAHFK
                     YYGFYGENRHFIDCLQENRLPETHLGDAVKTMELVERIYRSQI"
     gene            3226..4926
                     /locus_tag="PLY56_01220"
     CDS             3226..4926
                     /locus_tag="PLY56_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20129.1"
                     /translation="MSDNPQRPCDPARYRALSLHREPMTEQSGKFPFPSRAELRRVAL
                     AAAALIAFTCLPYLWGWLLRPADFMGHVYNADDASVHLAWMRQAAEGRWLFTDRFTSE
                     EQQPQFFHLFYLVLGKLAGLFGDSYRVFISFYHLARVVGGWLFLMAAYLLAAYIFPDA
                     RTRWLGFWTVGLSSGLGFWLELLLGKADRSCDYGPGLLMPEAITFLSLYLFPLFAVSI
                     LVLVGLYLLVLRAFDTGRVAPAIGAGVLGLVLGNIHSYDLLPVYAVLAVYVITLGVQR
                     RALPRREILLAALVVAISVPAPAYQYLVFRSNPVFQQKALTPTLSPSVMNYALTYGLV
                     LVAACVGAWHLRRRPHHRGNFLITWFIVTLAMAYLPVSFQRKMIEGAHIPLALLAASG
                     WLALTSRVRLPGAAATLLWIVLVIPSNLGVMADSIRRLGDNNAEGVAYLLPPYYLDSD
                     QRAVLEWLRENARPEDVVLSSPLDGCYMPPYCGVTTYVSHWAETINVQRKLADLRGFL
                     ASYTADEWRIQFLRENRIRYFYHGPAEAAMGGFDPSGASYLRLVCQSGPVALYEVVLP
                     "
     gene            4923..7292
                     /locus_tag="PLY56_01225"
     CDS             4923..7292
                     /locus_tag="PLY56_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ20130.1"
                     /translation="MRRPSLRDAGAYACFLLLACVLLWPVVLRGEALLPLQYAASFYP
                     WKQAGEGLPQPGERLNGEPLPWNALLADSVLQYLPWRHFARESLHAGYLPLWNPHQFC
                     GTPFVANYQSALFYPFNLLFWVLPVVHAFGVSAILHLFLAGAFVYLYLRGRGVDRGPA
                     LLGGITFELSGFVVAWLELPTAVNVAVWLPLAFHLYERSRAGAGALRGLATTIPLAMI
                     LLAGHPQFAFYALLGFALYALVHPPAGRESAQTGLIPRAGGWLGGLLVCAGVPLALAG
                     LIAAAQVLPMLELSRLSHRAGGSLLSLDASGYRNQALPLWSAVVLALPDFFGNPMRGD
                     FWGPANRAEFVGYVGVLPLLLAALMAVSLLVPSIRRPWLAALPPGGARHVAFFLGLAL
                     VAVAGSFRTPIGDVLYQIVPGLGRLGSPGRMLYLFTVALAMLAGFGAHALMRIPTRGK
                     PVALAAAVVGVGGMSVWLLASLMAAALGAGDVLGATAGAAFHFCALLVLAGGVLAGSG
                     RLPRPTAGALLLLVLVGDLLANAWGQNPTAPAALAYPPNPVVTVLKQDPEARFLAITP
                     RWSLGEYPITVLPPNTATAVGLFDVNGYDSLYGIAYKSFLNRLAGKETSPAANGNMIL
                     FESAEPEVLRALGCRYVVTLGPLGEEHFELLASGVVNVYRLRGACPRAFLEGGPGQAT
                     LSTMAPTRVMMSLDARGPGQVVLLESYFPGWRAWVDGQPAPVELAREVFRAVPVPAGA
                     RRLELHYAPMSFRLGLFLTLLGVGFLTGAGIARASAKKKVLSPVERAPQ"
     gene            complement(7322..7678)
                     /locus_tag="PLY56_01230"
     CDS             complement(7322..7678)
                     /locus_tag="PLY56_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20131.1"
                     /translation="MGNLRIGGSTREEQVDGVTHINIAGIRYLPLAPSTAVREAVREY
                     SDMRLAPWCETLSYMAAYPSVVFCRSDWERVIRAASGAATDNRLSARTRMTFAHIRDV
                     LSRALPGRLRDSAATL"
     gene            8255..>10141
                     /locus_tag="PLY56_01235"
     CDS             8255..>10141
                     /locus_tag="PLY56_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ20132.1"
                     /translation="MRKARGAWSLWSRGAARVLLGLIVAWGLVAPRVAAADPDPGAGE
                     LRGVWIHTYSPYDWDRVMRQLRDTGFNAVFVRVGRGGNVLYPSSLLPMDAWAEKAGGD
                     ELKRAIQAAHRYGLDFHAWRVCYHMGSAPKEYRARMAAEDRLARDPDGKQALYANPAD
                     PRNNELEYAAVMEVVRKYDIDGIHLDYIRYPSDPHYNFDYGPRSRAEFEKATGQKVAQ
                     WPEDVISGPLKRAYEEFERECVNSLMERVYRGVKREKPRVQVSAAVWRNHRRYRAVIK
                     QDWPLWIERGWMDFVVPMDYTADDEVYAATVKEQVAMASGKMPVVIGIGAWLLKDPAH
                     LVRQVELARQAGASGHVLFAYNARGIDAMLDALAKGPHRLPAKPAYRAPRICFELPDA
                     IARKDAPHAVEAERPFSLAAAWKAGRVDRSRGVQLTLETPGGRCLAQVGQLSPRGELR
                     AEPLAPAGLFQVVARGVLGGMEVVTRGPLLEGRPAAEIEALRAQENPPHVPDGALPRV
                     AVYEGGMGAAGILAALEQSGQVRAFSLHRLRPDHLAAADAVILPQLRDVADLTRDAEE
                     ALRAWVASGGTLLLTHDAVGFRWHPAPFPEIGVGAALARRQPLLVTTRGADAWSLEYA
                     YSDHV"
BASE COUNT         1549 a         3201 c         3462 g         1929 t
ORIGIN      
        1 aacgagccgg atgggtgcgt gccgaggaga gctgcgatgc cacggaatgg gagacgcaat
       61 ctgccgccgg agttccacgt cggggttccc gtggtggggc cggagggtga agtaggcacc
      121 atcacccggg tggtggtcga tcccgatacg agcgcggtca tcgatatcat cctccgcttg
      181 cgcaacggga gcgaatcgcg cgaggtggtc ttgcccgcgg atcgcgtgct ctcttcggat
      241 gcccacgaga cgcgcgtcga tcttacccgc gaggatgcgc gccgcctgcc ggacctggaa
      301 gaggtgcgct tgcggaagcc cgaggaaggc tgggaggggc ttccgggcga tacaccggat
      361 gtcgtctatt tctggatgcc tcggagcgcc gtggaggcgt tcgttccccc gagcatcgtg
      421 ccggagccga acgtggaggg gacgcgaaac gtgcccgagg ggagcgtggt cgtctctgct
      481 gacctggatg ttgtgtgcgg cgacgaggtg atcgggcacg tcgatggggt cgtcacggat
      541 gccgagggcg atcacgccac gcatctgatc gtggagcgag gtcttctgag gaaggaccgc
      601 catctcgtgc ctgtagaccg cgtccgcgag gtgacggatt cgagggtgcg gctggagtgc
      661 gatgcacgcg ggctggccgc ctatccgcgc taccaggagt gaaccatgcc gcgcgacatc
      721 acgccactga ccggcctgcg cgagaatctg atccttcagg aggagaagat tctccaggcg
      781 tggagggccc tggtgccgcc ggagcagacg ctcctcgccg aatcgctcgc cgattcggtc
      841 agggatctgc ttctcctgat gtatgagtgc ctctccggca taggaacgga ggcagggacc
      901 gacacccggc accttgccta ccagatcgcc cagcaagtgc tggaagagca cgactatcag
      961 acgctgatca acgtgttccg cgacgcgctc caggaggcgg tgatggatta caccgctctc
     1021 accagtggag acgcggcgcg caacctcatt gccttcttca cgatagtcac cgatgcctac
     1081 tggctggcct attcggatgg gctgcgcaag gcgattcgcc atcaatatcg ggagcggctg
     1141 agcaatgagc tgcgcgtggc caagcggatt caacagcgcc tcctgccgaa ggtgatcccg
     1201 cagattcctg gctgggagat tgccggccgg cttgtgcccg cgctggaggt cggcggcgac
     1261 tactggagcg tgaagcacta tgcgaacgat ggcgtcatca cctgcaagct ggcagatgtg
     1321 accggccacg ggattggcgc cgccatcctt gttgccgccg tgaagttcat ctcgggcggc
     1381 ttctaccgag gcgcgccgtc ggcctcgtgg gtgatggagc ggaccaacca cgtcctggtg
     1441 gtagagaccc ccagtgacat catggtcacc atggtctata cgtggattcg ccccgctacc
     1501 gggcagatca ccctggtgaa tgctggccac ggtccggttt tcgcatgccg cgatggccag
     1561 gtgcgaaccc tggcgcccac cggccccgcg ctcggtttgc tcgagtcgcg gtattcggag
     1621 gtagagctcc agtttcggcc tggcgatatc ctcttcttct gctcggatgg catcatcgag
     1681 gcgcgaagcc gtgatccggc aaaggcgcgc gagatgttcg gtacggagcg gttgcatgaa
     1741 gttgtctgcg gcttctcgca cctccccgcc gctgagatcg ccgataaggt ggtgcagact
     1801 gccatcgact tttccggaac tcccaacgac gacatgtcgc tcgttgtgat caagcgcgtg
     1861 tccgacgatg catgagaagg cacgcgcccc cttcgcgcgc cctctcctgc tcagccaagg
     1921 agttgcgctc tccagaggcg aaagggatcg cgtcggtaac cgcgcgaccg cgacagaccg
     1981 cgaagtcgca gagccggaag gagacgacgg cagtgagaaa gatacgtgtt ggcctgatcg
     2041 gagccggcgg gatggcgaac agtgttcact atccatccct cgccgagttc ccggatgtgg
     2101 aaatggccgc cctgtgtgac ctggacgagc agaagctgca ggcaacggcg gcgaagtttg
     2161 gcatcgagcg cacgtacacg gattaccgca agatgctgga tgaggtggat ccggaggcgg
     2221 tttacgtgct catgcctccg catcaccttt tcgacctcgc gatcaatatc ctgaaccaga
     2281 agcgtcacct cttcatcgag aagccgcccg gcgtgacgcg cgagcagacg cgcaacatgg
     2341 cgctgacggc cgagcgcaac ggctgcctca ccatggtcgg gttcaaccgg cgcttcatac
     2401 cgctcatccg cgaggtgcgc aagcgtgtgg atgagcacgg gccgatgctg cagtgcgtgg
     2461 ccactttcta taagaacagt gttggcgcgg ggccctacta tggtggcgcc atcgatattc
     2521 tcacctgcga cgccgtccat gccgtggata tgctccgcta catgggtggc gaggtggcgg
     2581 ccgtcgcggg cgatgtgcgc gcgttgcacg ccacttacga caactccttc aacgctctcg
     2641 tgcgctttga gaacggatgc accggtgtgc tcctgacgaa ctgggtggtg gggaagcgcg
     2701 tccatacgtt cgagatgcac gccaaaggct tctcggcgtt catcgatggc gatacccagg
     2761 cgacgctcta ttccgggaac aacgatccgg gcgaagtcct cgactcgcgc gcggtggccg
     2821 ggagcgatgc ccacttcaaa tactacggct tctacggcga gaatcgccac ttcatcgatt
     2881 gcctccagga gaaccgcctg cccgagacgc accttggcga cgccgtgaag acgatggaac
     2941 ttgtggagcg gatctaccgg tcccagatct aaggcgcggc cattggcccg ccgctgatgg
     3001 cactaggcgg aatgcccgac gagcgggcaa cagcttatcc cgctctgcat cagccgctgt
     3061 ttcgtcgggc attctgccag ccggccattc cgtgggcact ccccccaggg caagccgcgt
     3121 gagacgggcc gatgccgccg cggacgcgga ttggtgggga tggcgcgcct ttcagggcgt
     3181 tgcccgcgga agatctggag tgaggaaccg caccgtgttc acccgatgtc agacaaccca
     3241 caacgaccgt gcgacccggc gcgctaccgc gcgctgtcgc ttcatcgcga gccgatgacc
     3301 gagcaatccg ggaagttccc gttcccctcg agggccgagc tgcggcgggt cgccctcgcg
     3361 gccgccgctc tgatcgcgtt cacctgcctc ccgtatctct gggggtggct gctccggcct
     3421 gcggatttca tggggcatgt ctataatgcc gacgatgcca gcgttcacct cgcctggatg
     3481 cgccaagccg ccgagggccg ctggctcttt accgaccgct tcacctcgga agagcagcag
     3541 ccgcagttct tccacctctt ctatctggtg ctgggaaagc tggccgggct ttttggcgac
     3601 tcctaccggg tgttcatcag cttctatcac ctggcgcggg ttgttggtgg gtggctgttt
     3661 ctcatggcgg cctaccttct ggctgcctac atctttccgg atgccaggac gcgctggctc
     3721 gggttttgga cggtcggtct ctcatcgggg cttggcttct ggctggagct gcttcttggc
     3781 aaggccgacc gctcgtgcga ctacggcccc ggcctgctga tgccagaggc gatcactttt
     3841 ctcagtctct acctcttccc gctcttcgcg gtgagcatcc tggtgctggt cggtctctac
     3901 ttgctggtgc tgcgcgcctt cgataccggg cgggtggcgc cggcgattgg ggcgggtgtc
     3961 ctcggcctgg tgctggggaa catccattcc tacgatctgt tgcccgtcta cgccgtgctc
     4021 gcggtctacg tgatcaccct gggcgttcag cgccgcgcgt tgccgcgccg ggagatcctc
     4081 ctggcggcgc tcgtcgtggc gatctcggtg ccggcgccgg cgtatcagta tctcgtcttt
     4141 cgatcaaacc cggtcttcca gcaaaaggcg ctgacgccga cgctctcgcc cagcgtgatg
     4201 aattacgcgc tcacgtacgg tctggtgctc gtcgcggcgt gtgttggcgc ctggcatctg
     4261 agacgccggc cccaccaccg cggcaatttc ctgatcacct ggttcatcgt cacgctggcg
     4321 atggcgtatc tgcctgtgtc gttccagcgc aagatgatcg agggcgcgca cattccgctg
     4381 gcgctcctgg cggcctcggg gtggctcgct ctgacgtcgc gggtgcgcct gccgggcgct
     4441 gcggccaccc tcctgtggat tgtgcttgtc atcccatcga atctgggggt gatggccgac
     4501 tccattcgca ggctcggaga caacaacgcg gagggcgtgg cctacctgct gccgccttac
     4561 tacctggact cggaccagcg agccgtgctg gagtggctgc gcgagaatgc ccggcccgag
     4621 gatgtggtcc tgagctcacc gttggatgga tgctatatgc ctccttactg cggcgtgacg
     4681 acctacgtca gccattgggc cgagacgatc aacgtgcagc ggaagctggc ggacctgcgt
     4741 ggcttcctgg ccagctacac cgcggacgag tggcgcatcc agttcttgcg cgagaaccgg
     4801 atccgctatt tctaccacgg accggctgag gcggcgatgg gtggattcga tccctcgggg
     4861 gcgagctacc tgcgcctggt gtgccagagc gggccagtgg cgctgtatga ggtcgtcctg
     4921 ccatgagacg accgtcgctc cgcgatgccg gcgcgtatgc gtgcttcttg ctgctggcgt
     4981 gcgtgctgtt gtggccggtg gtgctgcgcg gcgaggcgct gctcccgttg cagtacgccg
     5041 cgagtttcta cccctggaag caggcgggag agggactacc gcagccgggt gaacgcctga
     5101 acggggaacc gttgccgtgg aacgcgctcc tggccgacag cgtgcttcag tacctgccgt
     5161 ggcggcattt cgcgcgcgag agcctgcacg ctggctacct tcccctgtgg aacccgcacc
     5221 agttctgcgg cacgcccttc gtggccaact accagtcggc gctcttctac ccgttcaacc
     5281 tcctgttttg ggtgctgcct gtggtccacg cctttggcgt gtcggcgatc ctgcacctct
     5341 tcctggccgg cgccttcgtc tatctctatc tacgggggcg cggcgtggac cgcggcccgg
     5401 ccctcctcgg cgggatcacc ttcgagctga gcggcttcgt ggtggcgtgg ctggagctcc
     5461 cgaccgccgt gaacgtcgcc gtctggctgc cgctcgcgtt ccacctctat gaacggagcc
     5521 gggccggagc aggcgctctg cggggcctgg caaccacgat tcccctggcg atgatcctcc
     5581 tggccggcca cccgcagttc gccttctatg cgctgctcgg ctttgctctc tacgcgctcg
     5641 tccatccgcc tgccggccgt gaatccgccc agaccggcct cattccgcgc gcgggcgggt
     5701 ggctgggagg gttgttggtg tgcgcaggcg tgccgcttgc cctggcgggc ctgattgctg
     5761 cggcccaggt gcttccgatg ctcgagctct ctcgcttgag ccaccgcgct ggcggttcgc
     5821 tgctctcctt ggacgcatcg ggctatcgga accaggccct ccctctctgg tccgccgtgg
     5881 tgctggcgtt gcccgacttt tttggcaacc cgatgcgcgg cgatttctgg ggtccggcaa
     5941 accgtgcgga gttcgtgggc tatgtcgggg tgctcccgct tcttctcgcg gcgctcatgg
     6001 cggtgagcct cctggtaccc tcgatccggc gtccgtggct ggcggcgctg cctcccggcg
     6061 gcgcgcggca tgtggccttc tttctggggt tggccctcgt ggccgtggcc ggatcgtttc
     6121 ggactccgat tggtgacgtg ctctatcaga tcgtgccggg cctcggacgc cttggctcgc
     6181 ccgggcggat gctctacctc ttcactgtgg ccctggcgat gctggcgggc tttggtgcac
     6241 acgccctgat gcggatcccg acgcgcggga agccggtggc gctggcggca gcggttgttg
     6301 gtgtgggcgg catgagcgtg tggctgcttg cctccttgat ggcggcggcg ctgggcgcgg
     6361 gggatgtgct cggcgcaact gctggcgctg ccttccactt ctgcgcgctg ctggtgctgg
     6421 ctggcggagt actggccgga agtggacgcc tcccccggcc aacggcaggg gcgctgctcc
     6481 tcctggtgct ggtgggcgac ctcctggcga acgcctgggg gcagaacccc acggcgccgg
     6541 ccgcgctggc ctacccaccc aacccggtgg tgacagtgct gaagcaggat ccggaggcgc
     6601 gtttcctcgc catcacgcca cgctggagcc tcggagagta tccgatcacc gtgctgccac
     6661 ccaacaccgc cacagccgtg ggcctattcg atgtgaacgg ctacgactcg ctctacggca
     6721 tcgcctacaa gagcttcttg aaccgcctgg ccggcaagga gacgagcccg gcggccaatg
     6781 ggaacatgat cctcttcgag agcgcggagc cggaggtgct gcgcgcgctt ggctgccgct
     6841 acgtcgtcac tttgggcccg ttgggagagg agcacttcga actgctggct tccggcgtgg
     6901 tgaacgtcta ccggcttcgc ggtgcgtgcc cgcgcgcgtt cctcgagggc gggccaggcc
     6961 aggcgacgct ttcgacaatg gcgcccacgc gggtgatgat gagcctcgat gcacggggcc
     7021 caggccaggt ggtgcttctg gagagctatt tcccgggatg gcgcgcgtgg gtggatggcc
     7081 agccggcgcc cgtggagctg gcgcgcgagg tgttccgcgc ggtgccggtg ccggccggcg
     7141 cccgtcgcct ggaactccac tacgcgccga tgagtttccg cctcggcctc ttccttactc
     7201 tgttgggggt gggcttcctc accggcgccg gcatcgcgcg tgcgagcgcg aaaaaaaagg
     7261 tgctctctcc agtggagcga gcaccacaat aaaaaagatg gatgatactg atgaggcttg
     7321 gttacagagt ggccgctgaa tcgcgcaggc gcccaggaag cgcacgggaa agaacgtcac
     7381 ggatatgcgc gaaagtcatg cgtgtccttg cggagaggcg gttgtcggtg gcggcaccac
     7441 tcgccgcgcg gataaccctc tcccagtcgc tgcggcagaa aaccaccgag gggtacgcgg
     7501 ccatgtagct gagcgtctcg caccagggcg ccaggcgcat gtcactgtac tcacgaaccg
     7561 cctcgcgcac cgcggtggag ggcgccaacg gaaggtaacg gatcccggcg atgttgatat
     7621 gtgtcacgcc gtccacctgc tcctcccgag tactgccgcc gatcctcaga ttgcccatag
     7681 gctgtctcac ctcccttcag gcggtctggc ttccctacta ccaccgtatg tattatactg
     7741 gcaccgcatt aaaacaagat tagaaaagga ttagaaacct ctaatcttgt agggggagcc
     7801 tgtcagggct ccggatcaga cgcgggtgac cgctgcccgc acggatgagg gtgcgcgccc
     7861 ccggggccat cgcaagctgt gacccgcccc gatccgtccg gcgctgcccg gggccggcgg
     7921 gaaccccaac cctcggtctc cacgcattcg cgatagcctc catcattccg tccgcgctcg
     7981 tccccttcat acgcgagggt gttacacccg aggtgtcaat gccgttgcta gggtgccagg
     8041 ggagcaggca gtacggccca ggccaaggag accccgggca tcgcgagcgg tgtgatatca
     8101 gtggggggga cctggcttcg gcgcttgcac aggaacgaac caggacgccg ttgtttgcgt
     8161 ctttagagag ctagccacct ggtggtacgg aagcggatct cgttgcatgg cgatgcctgg
     8221 agaggatgtg gatgtgaggg gaactggaaa gagggtgaga aaagcgcggg gagcatggtc
     8281 gctgtggtcg cggggtgcag cccgggtgtt gctgggtctc atcgtcgcgt ggggcctcgt
     8341 ggcgccgcgc gtcgccgcgg ccgatccgga tccgggggcg ggcgagctcc ggggcgtctg
     8401 gattcatacc tatagcccat atgattggga ccgcgtgatg cgccagctgc gcgatacggg
     8461 cttcaacgcc gtcttcgtgc gcgtggggcg cggggggaac gtgctttatc ccagttcgct
     8521 cctgccgatg gacgcctggg ccgagaaggc aggaggcgat gagctgaaac gcgccattca
     8581 ggcggcccat cgctacgggc tcgacttcca cgcctggcgg gtctgctatc atatgggctc
     8641 ggcgccgaag gagtatcggg cacggatggc cgccgaggac cgcctcgcgc gcgatcccga
     8701 cggaaaacag gcgctctacg cgaaccccgc agacccacgc aacaacgagc ttgagtatgc
     8761 cgcggtgatg gaagtcgtgc gtaagtacga cattgatggg atccacctgg actatatccg
     8821 ctacccgagc gacccgcatt acaacttcga ctatgggccg cgttcgcgcg ccgagttcga
     8881 gaaggcgact ggccagaagg tggcgcagtg gccggaggat gtgattagcg ggccgttgaa
     8941 gcgcgcctat gaggagtttg agcgggagtg cgtcaactcg ctcatggagc gggtttaccg
     9001 aggtgtgaag agagagaagc ctcgggttca ggtctccgcc gcggtgtggc gcaaccaccg
     9061 ccgctatcgc gccgtgatca agcaggactg gcccctctgg atcgagcgcg gctggatgga
     9121 ttttgtggtt cctatggact acaccgcgga cgacgaggtg tatgccgcca ccgtgaagga
     9181 gcaggtggcg atggcctctg ggaagatgcc ggtcgtcatc ggcattggcg cgtggctcct
     9241 gaaagatccg gcgcacctgg tccgacaggt ggagctggcc cgtcaggccg gagcgagcgg
     9301 gcacgtgctt ttcgcctaca atgcgcgcgg gattgatgcg atgctggatg cgctcgccaa
     9361 agggccccat cgcttgcctg cgaagcccgc gtaccgcgcg ccacgaatct gcttcgagct
     9421 tccagacgcc attgcgcgca aggatgcccc ccatgccgtc gaggccgagc gtcccttctc
     9481 acttgccgcg gcatggaaag cgggcagggt ggatcgctcc cgcggagtcc agctcaccct
     9541 ggagacgccg ggtggccggt gcctggcgca ggtgggccag ctctccccgc gcggcgagtt
     9601 gcgggcggag ccactggccc cggcgggcct cttccaggtt gtggcgcgcg gggtgctggg
     9661 cggcatggag gtcgtcacgc gtgggccgct cctggagggc cggccggcag cagagattga
     9721 ggcgctgcgt gcgcaggaga acccgcctca cgtgcccgat ggcgccctcc cgcgtgtggc
     9781 ggtgtacgag ggtggcatgg gggcggccgg tatcctcgcg gcactggagc agagcgggca
     9841 ggtgcgggcg ttctccctcc atcgtctgcg tccagaccat ctggccgcgg cggatgcggt
     9901 gattctgccc caacttcgcg acgtggccga cctgacgcgg gatgcagagg aagccctgcg
     9961 tgcctgggtg gcctctgggg gcacgctgct cctgactcac gatgccgtcg gatttcgctg
    10021 gcacccggcg cccttccccg agatcggcgt aggtgccgcg cttgcccggc gccaaccgct
    10081 gctcgtgaca acgcgggggg ccgatgcctg gtcgctggag tatgcctata gcgaccacgt
    10141 g
//
LOCUS       DAOSCV010000025        10129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4357_length_10129_cov_3.523824, whole genome shotgun sequence.
ACCESSION   DAOSCV010000025 DAOSCV010000000
VERSION     DAOSCV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4357_length_10129_cov_3.523824"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2146)
                     /locus_tag="PLY56_01240"
     CDS             complement(<1..2146)
                     /locus_tag="PLY56_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20133.1"
                     /translation="MRHYGILLLVLLGSAYLMAGLPARAQAAPVKPSFLEEECPEEFT
                     LLGQVRGLSGKKSATLFFNYTDAAHTLAVQLSRNQAVVVRRDGGAGQRLASVALPVGR
                     QDSLEFAVKRRRWRVTLICNDRVVAQCYDDSLAGGRAGWQADPGVTMDLRLQATEPVQ
                     FADDFTRGPGEVGQWEILAGTGQVAEVAIARHGKDVGRYSSNAFSWTASARPIAMSVA
                     GYWFWDNYAVDVSVKPEGRGAVGIAAYLKDADNFLLFRWTAATEGQPGSGAREILRVE
                     GGKATLLASRPGGFQPRQWYRLRFTACEGVFTASIDGEEVCRVRDAAFGHGRVGLYAR
                     DTDRVVFDDVRVVATDAFSDSFDAHEPGKWQNLGMAWKADTRGRARVSGAGEGITITG
                     RPDWSGYAFGADVTPGKAEAVGLVFAYRGPGEYALFRVPASGKGRAVLLRVQGGKPVV
                     VAESPAAVVAPRRSRLKLELRDGQATGFIGNRPVVAGVDPAFGEGKAGFYARGSAGVR
                     FEKASVWFSVPEGDEPRIEAQFAREQTMAGWASASSSWSRDPGGSGLFWHTGFFPGDA
                     SVSWDLPGGNAPLDLMLALNADGSRAETGYQAVLSVNAQGAARLELRRGGQSVAAQSG
                     RWEPGSPLRVWRSGSLIAAALGDALVAQYQDPQPLTGGRIGLRCSREVNFASVYLSTL
                     HVLDCTFSGPPTEWWAPRGDWDVVQRWPCDERW"
     gene            2505..4271
                     /gene="argS"
                     /locus_tag="PLY56_01245"
     CDS             2505..4271
                     /gene="argS"
                     /locus_tag="PLY56_01245"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ20134.1"
                     /translation="MKSIPERIEAIVRQALIHLLGPDGAEVDPLVRQTQDPKFGDYQS
                     NVAMGLAKRLGKKPRDVAAELVAAMDVGELSEPPSIAGPGFINFRIRPSFLAAELEAI
                     QADPRLGMPQVADPQRIIVDFSSPNLAKEMHVGHLRSTIIGDAIARLLEFEGHDVLRL
                     NHVGDWGTQFGMLIQHVRETQPDVLEHPERFHIDDLESFYREAKARFDADSEFADAAR
                     RAVVDLQSGDPVAHRLWEVFCTESLRHAHEIYERLGIRLVDRGESFYNPMLPQVVAEF
                     QERGLAVEAEGAVCVFLPGFTNREGGPLPLIIRKSDGGYNYETTDLAALRHRVDVEKA
                     QRVIYITDIRQRQHFEMCFAAARAIGWVPDDVQLDHVGFGMVLGPDRRPFKTREGGTV
                     KLKDLLDEAVSRAAAVIDAEDERRSEMSPEEKAEIARVVGLGAVKYADLSHNLTSDYV
                     FDWDTMLAMDGNTAPYMLYAYARVRSIGRRAGVEFDSLPADLPLVLEHESEIALAKEL
                     LRFSHVLQQVSADLRPHLLTDYLYGLSRAFSTFYDRDRGVRVIDAEPESVRLSRLRLC
                     DLTARTLRLGLHLLGIEVLEQM"
     gene            complement(4396..5499)
                     /locus_tag="PLY56_01250"
     CDS             complement(4396..5499)
                     /locus_tag="PLY56_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOJ20135.1"
                     /translation="MMNKQVMPTIVGAVILALLGGYVYFFERGKAPEEGGARPLSRVL
                     GYKADRVWKLQIDRENDPIWIVRQKDRRGQMWRMEKPLKALADGEEATRLITELSDTT
                     VERVLTENTSDLKPFGLDQPAWEITLATDDGKTHTLLVGGKDPGGSLLYLKPKERSDI
                     LVVSSYSLDGLQMKVADELRDKSVIGFKKEEVQRFELTAEGQTIRVERAGADAWKITA
                     PVVAKARKEKVNSILMTFERLKGTKIVADSPKDLKPYGLDKPSATVAVWVKGEKEPRV
                     ALLGSKDSSGDLYAKGAHNPAVVTVYSYVLSDAQSKVEDIKEPDPEPEKKEKKPANEP
                     GNAPAEKPAANEPAANGPTIDKPIPKKEAPAKE"
     gene            complement(5653..7212)
                     /locus_tag="PLY56_01255"
     CDS             complement(5653..7212)
                     /locus_tag="PLY56_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOJ20136.1"
                     /translation="MSSRIHHTELSQRDHLGIALGWVSLALLAVGLIGYLGNNTLVLW
                     VKIVLGLGVVAGAGYLAIRGKAAKAAIFNPSTGAGINTTALTLIVLAILVMVNYIAGR
                     HKYQVDLTRQHLYTLSPQTKEVVKNLKDEVQITAIYQLAGRRGAEGQRARDLLKQYKD
                     ISPKIKFEMLDPVAEPAKAMAKGVQTMDVIWVESGGRKETVYTVGEQEISSAILKVTR
                     PEKKKVYFLFGHGERDIESYERDGLSDAKSLLEKLNYETSKLILQTVKEIPADCSVLV
                     VAGPKNGLPANEQKMLAKYLENGGKMLLMLDPKSPVPTELVSPWGVEVGNNIVFDPRL
                     GPGQALVEKFEFHQITKDLQIALFPLARSVSPATTSPDGVTVLSLAKTPSMAWAETKL
                     EGPYQQDGDDLPGPVSLAVVAQKEQFADSPNQADDKKKSTRLIVVGNSTFATNAAFQA
                     VNLSDAEFFLNSVNWLAEEEALVNIPPKEPERNTITLMDSTSRWIFLTTVLIVPFGVL
                     LSGVFIWWRRR"
     gene            complement(7238..7951)
                     /locus_tag="PLY56_01260"
     CDS             complement(7238..7951)
                     /locus_tag="PLY56_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ20137.1"
                     /translation="MKNAIVIMRRELHSYFTSTIAYSVAAFFLLICGYLFFATVKVTQ
                     EATLLYLFHNAAVTLLLISPAMTVRLVAEERRSGSIELLMTAPVTDTQVIIGKYLGAL
                     VFYASMLLLTLQYPIILLRLGSPDGGPMLTGYIGLFLLGAAFLAIGLVASTLTKNQIV
                     AAVGAFSVMLILWVISWVGSTGASSTWSEVLRQLSLLDRFGNFTKGLIDSRDVIFFLS
                     IIGFCLFLSVRALGAVKSR"
     gene            complement(7948..8895)
                     /locus_tag="PLY56_01265"
     CDS             complement(7948..8895)
                     /locus_tag="PLY56_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ20138.1"
                     /translation="MIQVENLTKYYGEYAAIENVSFAVEKGEILGFLGPNAAGKTTTM
                     RILTGFLQPTSGKAAVAGFDVVSESLEARKRIGYLPENVPLYDDMTPRSYLQYAARLK
                     GVESRQVNKRVEEVMEATRITDHAEVLISKLSKGYRQRVGLAQALIHNPDVLILDEPT
                     VGLDPNQIVEIRQLIKGLGGDRTIILSTHILPEVEMLCGRIVIIHEGKVAAMDTPDNL
                     ALRLRRAQQIGVHVRGPEREVQAKLAALPGVLRVQVVRAGDSVVELLVDGEEKHDVRE
                     ALASAIVSNGWGLRHMAEVGMSLEEIFRQLTSEEKGVAA"
     gene            complement(9441..10028)
                     /locus_tag="PLY56_01270"
     CDS             complement(9441..10028)
                     /locus_tag="PLY56_01270"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ20139.1"
                     /translation="MPDSSLRAAKAELRARMTALRDALPAAWRAEADHAITQRLLSGP
                     EWTGVRVVFAYVSFRSEVGTRAILTAALESGRALLVPRVDRERREMQACRIHSLDELV
                     PGSWGILEPAEATPMEPAQIDLIVMPGLAFDRQGRRLGYGAGYYDRFLHGVRPDCIRL
                     GLAYEAQLLPAVPAGTSDERVSRVITEAGLYHCQG"
     gene            complement(10031..>10129)
                     /locus_tag="PLY56_01275"
     CDS             complement(10031..>10129)
                     /locus_tag="PLY56_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HOJ20140.1"
                     /translation="VSGKVKDDLQAIIQLVREKDFNLPLQFTNYRG"
BASE COUNT         1759 a         3390 c         3215 g         1765 t
ORIGIN      
        1 accagcgctc atcgcatggc cagcgctgga ccacgtccca atcgccccgg ggagcccacc
       61 actccgtggg cggcccgctg aaggtgcagt cgagcacgtg tagggttgaa agatagacgg
      121 acgcgaagtt gacctcccgg ctgcaccgga ggccgattcg tccgcctgtc aggggctgcg
      181 ggtcctgata ctgcgccaca agcgcgtcgc ccagggccgc tgcgatcagg cttcccgagc
      241 gccacacgcg cagcgggctg cccggctccc accggcccga ttgcgccgcc acgctctgcc
      301 caccgcgccg cagctccagg cgcgcggcgc cctgcgcgtt cacgctcagg acggcctgat
      361 agcccgtctc agccctggag ccatccgcgt tcagggcgag catcaggtcg agtggcgcgt
      421 tcccgccagg caggtcccag gaaaccgagg catcgcccgg aaagaacccg gtgtgccaga
      481 aaagccccga tccgcccggg tcgcggctcc aggaggagga ggcgctcgcc catcccgcca
      541 tcgtctgctc gcgcgcgaac tgcgcctcga tgcgaggctc gtcgccctcg ggcacggaga
      601 accacacgct cgccttctcg aagcgcaccc ccgctgaacc acgcgcgtag aagccagcct
      661 ttccctctcc aaacgcggga tcgacgccgg ccaccaccgg ccggttgccg atgaacccgg
      721 tcgcctggcc atcgcgcagc tccagcttca agcggctccg gcgcggcgca acgacggccg
      781 ccggcgactc ggccaccacc accggcttgc ctccctgcac gcgaagcagc accgccctgc
      841 ccttcccgct ggcgggcacc cggaagagcg cgtactctcc tgggccccgg tacgcgaaga
      901 ccagcccgac cgcttctgcc ttcccggggg tcacatccgc gccgaaggca tagccgctcc
      961 aatccggccg gcccgtgatc gtgatcccct cgcccgcgcc ggagacgcgc gcgcgcccgc
     1021 gcgtgtctgc cttccaggcc atccccagat tctgccactt gcccggctca tgggcatcga
     1081 atgaatccga gaaggcgtcg gtggcaacca cgcgcacgtc gtcaaagacg acgcgatcgg
     1141 tatctcgggc gtagaggcca acccggccgt ggccaaaggc ggcgtcacga actcggcaca
     1201 cctcctcacc atcgatcgac gcggtgaaga cgccctcgca cgcggtgaac cgcagccggt
     1261 accactgcct gggctggaag ccgcccgggc gcgatgccag gagcgtcgcc ttcccgccct
     1321 cgacacggag gatctcgcgc gcgccggaac cgggctggcc ctccgtggcc gccgtccagc
     1381 ggaagaggag gaagttatcg gcatctttca ggtaggccgc gatccccacg gcgccacggc
     1441 cctccggctt cacggagaca tccaccgcgt agttgtccca aaaccagtag ccggccaccg
     1501 acatggcgat ggggcgcgcg ctcgctgtcc aggagaaggc gttgctgctg tagcggccca
     1561 catccttccc gtgcctggcg atcgccacct cggccacctg gcccgtgccc gcgagaatct
     1621 cccattggcc gacctcgccc gggccgcgcg tgaagtcgtc cgcgaattgg accggttctg
     1681 tcgcttgcag gcgcagatcc atcgtgactc ctggatccgc ctgccagccc gcgcgccctc
     1741 cggcgagcga atcatcgtag cactgggcga ccacgcggtc gttacagatc agcgtgaccc
     1801 gccagcgccg gcgtttcacg gcgaactcca aggaatcttg cctgcccacc gggagggcca
     1861 ccgaggccag gcgctgcccg gcgccaccat cgcggcgcac gacgaccgcc tggtttcgag
     1921 agagctgcac cgcgagggta tgtgctgcat cggtatagtt gaagaagagc gtggcgctct
     1981 tcttgccgct cagaccgcgc acctgcccca ggagggtgaa ctcctcgggg cactcctctt
     2041 ccaggaaact cggcttcacc ggagcggctt gcgcgcgcgc cggcaacccc gccatcaggt
     2101 acgcggaccc cagcaagacg agcaagagta tcccgtagtg acgcatggaa tcccggccgc
     2161 tggcggagat ccagagttgc cgggcgcaga agaaggcccc tcgcgccaaa tggcggctcg
     2221 cataggatag acagttcacg attgcatccc caatgccggt cggtgggcgc cggtcgcgct
     2281 cgcgtgtccg gccaccctgc ctcctgcgcg cgttcgcatg cgcacgctca ctatagccac
     2341 aaacccgata ggacaaacgg tgctgcggca atcgcctgga acagcacgcg ctatgatctt
     2401 accccttccc ggaatggaac tcctgccagg atgcaggctg cgagcggctg gataggacgc
     2461 gccgaaccgg ccacgaccgc ctcaaaatgt gctatactag aggcatgaag agtattcccg
     2521 aacgcattga agcgattgtc aggcaagccc tgatccacct tctcggcccc gacggcgctg
     2581 aggtagaccc gcttgtccgc cagacccagg acccgaagtt cggagactac cagtcgaatg
     2641 tggcgatggg cctagcgaag cgccttggca agaagccgcg tgacgtcgcc gctgagctcg
     2701 tcgcggcgat ggatgtcggc gagctcagcg agccgccctc gattgccggc cccggtttca
     2761 tcaacttccg catccggccc tctttcctgg ccgcggagct ggaggcgatt caggccgacc
     2821 cgcgcctggg gatgccacag gttgcggatc cacagcggat catcgtcgac ttctccagcc
     2881 cgaacctggc gaaggagatg cacgtcgggc acctgcgctc cacgatcatc ggcgacgcga
     2941 tcgcgcgcct cctggagttc gagggccacg atgtcctgcg cctcaaccac gtcggtgatt
     3001 ggggtaccca gtttggcatg ctgatccagc acgtgcgtga gactcagccg gacgttttgg
     3061 agcaccccga gcgtttccac attgatgacc tggagagctt ctatcgcgag gccaaggcac
     3121 ggtttgatgc cgattcggag tttgccgatg cggcccgccg cgccgtggtc gacctccagt
     3181 cgggcgatcc ggtggcccac cggctatggg aggtcttctg caccgagtcg ctgcgccatg
     3241 cccacgagat ttacgagcgc ctcggcatcc ggctggtgga tcgcggcgag agcttctaca
     3301 acccgatgct gccacaggtg gtggccgagt tccaggagcg cggtctggcg gtggaggccg
     3361 aaggcgccgt gtgcgtcttc ctccccgggt tcaccaaccg ggagggtggc ccgctgccgc
     3421 tcatcatccg gaagtcggat ggagggtaca attacgagac caccgacctc gcggcgctgc
     3481 gccaccgcgt cgatgtggag aaggcccagc gggtgatcta tatcaccgat atccgccagc
     3541 gccagcattt cgaaatgtgc ttcgcggcgg cgcgcgccat cggctgggtg cccgacgacg
     3601 tgcagctcga ccacgtcggc tttggaatgg ttctcggccc cgaccgacgc cccttcaaga
     3661 cgcgtgaggg gggcacggtg aagctgaagg acctcctgga cgaggccgtc tcgcgcgccg
     3721 cggcggtgat cgacgccgag gatgagcgcc ggagcgagat gagccccgag gagaaggcgg
     3781 agatcgcgcg agtcgtgggc ctgggagcgg tgaagtatgc cgacctgagc cacaatctga
     3841 cgagcgatta cgtcttcgac tgggacacaa tgctggcgat ggacggcaac acggcgccct
     3901 acatgttgta cgcctatgcc cgagtgcgga gtatcggccg gcgtgccggc gtggagttcg
     3961 attcgctccc ggcggacctt cccctggtgc tggagcatga atcggagatc gccctggcca
     4021 aggagctcct ccgcttctcg cacgtgctac agcaggtttc cgccgatttg cgcccgcatc
     4081 tcctgacgga ttacctgtat ggcctgagcc gggcgttcag caccttctac gaccgggatc
     4141 gcggcgtgcg cgtcatcgac gcggagccgg agagcgttcg cctcagccgg ctgcgcctgt
     4201 gcgacctgac ggcgcgcacc ctccggctgg gcctgcatct cctcggcatc gaggtgctgg
     4261 agcagatgta gcggcagcca accagggaga acgtgtttcc ctcggcagtt ccgccaccct
     4321 gtcgaaaaag aagccggccc ggactcgtgg gcgtgagtcc gggccggcat gtatttcctg
     4381 cgatccaatc gggcgctact ccttggcagg cgcctccttc ttggggatcg gcttatcgat
     4441 ggtcgggccg tttgccgcgg gctcgttcgc ggcaggcttc tccgcgggag cgttccccgg
     4501 ctcgttggcc ggctttttct ccttcttctc cggctccgga tccggctctt tgatatcctc
     4561 gaccttgctc tgggcgtcac tcaggacata ggaatagacc gtgacaaccg ccgggttgtg
     4621 cgcccccttg gcatagaggt cgccgctgct gtccttgctg cccagcaacg ccacgcgcgg
     4681 ctccttctcg cctttcaccc agaccgccac ggtagccgac ggcttgtcga gaccgtacgg
     4741 cttcagatcc ttggggctat ccgcgacgat cttagtgccc ttcaggcgct cgaacgtcat
     4801 caggatggag ttgaccttct ccttcctcgc cttggcaacc acgggggctg tgatcttcca
     4861 ggcatcggcg cccgcgcgct cgacacgaat cgtttgaccc tcggcggtca attcgaagcg
     4921 ctgcacctcc tccttcttga agccaatgac cgacttatcg cgcagctcat cggccacctt
     4981 catctgaagc ccatccagcg agtaggagga gacgaccagg atatcactcc gctccttggg
     5041 cttcaggtag aggagcgagc cgccgggatc cttgcccccg accaggaggg tatgcgtctt
     5101 cccgtcgtcg gtggcgaggg tgatctccca ggcgggctga tcgaggccga acggcttgag
     5161 atcgctggtg ttttcggtca acacgcgctc gaccgtagta tcggagagct cggtgatcaa
     5221 ccgcgtcgcc tcctcgccat cggccagggc tttgagcggc ttctccatcc gccacatctg
     5281 gccgcggcga tccttctggc gcacgatcca gatcggatcg ttctcacggt cgatctggag
     5341 cttccagacg cggtcggctt tgtagccgag cactcgtgac agtgggcgcg cgccgccctc
     5401 ttcgggtgcc tttccacgct cgaagaagta gacatagccg ccgaggagcg caagaatgac
     5461 cgcgcccacg atcgtcggca tgacttgctt attcatcatc tccctacctt ggaatgtgga
     5521 tcacgggttg gcgtgaggga gtggcaaggc aggagatggg ccggcggcct gggagagcgg
     5581 cagccatgcc gcctcccaag agtcaccgcg ccctccccca tctcctactc catcacgacc
     5641 ggtgctcccg gcttatcgcc ggcgccacca gatgaagacg ccgctgagga gcacgccaaa
     5701 cggaacgatc agcacagtgg tcaggaagat ccagcgcgac gtggagtcca tgagcgtgat
     5761 ggtgttgcgc tcgggctcct tcggcgggat gttgaccagg gcttcctctt cggccagcca
     5821 gttgacactg ttcaagaaga actccgcatc gctcaggttc acggcctgga acgccgcgtt
     5881 ggtcgcgaac gtcgagttcc cgacgacgat gagccgcgtg ctcttcttct tgtcgtccgc
     5941 ctggttgggc gagtcggcga actgctcttt ctgagccacc accgccagcg agaccgggcc
     6001 gggcagatcg tccccgtcct gctggtatgg cccttccagc ttggtctcgg cccaggccat
     6061 gctcggcgtc ttcgcgagcg agaggacggt gaccccatcc ggggatgtcg tggccgggct
     6121 gaccgagcgt gccagcggga agagcgcgat ctgcagatcc ttggtgatct ggtggaactc
     6181 gaacttctcc accagcgcct ggccagggcc caagcgcggg tcgaacacga tgttgttgcc
     6241 aacctcgacg ccccagggcg agaccagctc cgtaggaacc ggcgacttcg ggtcgagcat
     6301 caggagcatc ttgccgccgt tctcaaggta cttggcaagc atcttctgct cgtttgccgg
     6361 caggccattc tttggcccgg caaccaccaa cacggagcaa tcggcgggga tctccttcac
     6421 cgtctggagg atcagcttgc tcgtctcgta gttcaacttc tccagaaggc tcttcgcgtc
     6481 ggagaggccg tcgcgctcgt aagactcgat atcgcgctcg ccgtggccga agaggaagta
     6541 gaccttcttc ttctccgggc gggtcacctt gaggatggcg ctggagatct cctgctcgcc
     6601 gacggtgtag accgtctcct tgcgcccgcc ggactccacc cagatcacat ccatcgtttg
     6661 gacgcccttg gccatcgcct tggcgggttc ggccacggga tcgagcatct cgaacttgat
     6721 cttcgggctg atatccttgt actgcttcag gaggtcgcgg gcgcgctggc cttcggcgcc
     6781 acgccggcca gcgagctggt agatcgcggt gatctgcacc tcatccttca ggttcttgac
     6841 gacctctttc gtctgcggcg acagcgtgta gaggtgctgg cgcgtcaggt cgacctggta
     6901 cttgtgccgg ccggcaatat agttgaccat caccaggatc gccagaacga tgagggtcag
     6961 tgccgtcgtg ttgatgccag cgcccgtgct ggggttgaag atggccgcct tcgcggcctt
     7021 cccccggatg gcgagatagc ccgcaccggc caccactccg aggccaagca caatcttgac
     7081 ccagaggacc agggtgttgt tcccgaggta gccgatcagg ccgaccgcca gcagcgccag
     7141 gctcacccag ccgagcgcaa tccccagatg gtcgcgctgg ctcagttcgg tatggtgaat
     7201 gcgagaagac atcgcttcgc tttcctctct cctaacgcta tcgagacttc acggcgccga
     7261 gggcgcgaac cgataggaac aggcagaagc cgatgatcga caggaagaag atcacatctc
     7321 gcgaatcgat caggcctttc gtgaagttcc cgaagcggtc cagcagcgac aactggcgca
     7381 gaacctcgga ccacgtggag gaggcgccgg tgctgcccac ccaggagatc acccagagga
     7441 tgagcatcac ggagaaggcg cccaccgccg ccacgatttg gttcttcgtc agcgtggagg
     7501 ccacgagacc aatggccagg aacgcggcgc caagcaggaa gaggccaata tagccggtca
     7561 gcatcgggcc accatccggc gagcctagcc gcagcaggat gatcgggtat tgaagggtga
     7621 ggaggagcat cgaggcgtag aagaccagag ccccgaggta cttgccgatg atcacctgcg
     7681 tatctgtcac gggcgcggtc atcagaagct caatcgagcc gctccgccgc tcctcggcca
     7741 ccaggcgcac cgtcatggcc ggcgagatga gcagcagcgt caccgccgca ttatggaaaa
     7801 gatagaggag cgtggcttcc tgggtcacct tcacagtggc gaagaagaga tacccgcaga
     7861 tcaacaggaa gaacgcggcc acgctgtagg caatggtgga agtgaagtac gagtgcagct
     7921 ctcgccgcat gatcacaatc gcgttcttca cgctgcaact cctttctcct cggacgtgag
     7981 ctgccggaag atctcttcca ggctcatgcc gacctcggcc atatggcgca agccccaacc
     8041 gttgctgact atcgccgatg ccagagcctc gcgcacgtca tgcttctcct cgccatcgac
     8101 gagcagctcc acgaccgagt cgccggcgcg caccacctgc acacgcagca cgcccggcag
     8161 agccgccagt ttggcctgaa cttcacgctc tggcccgcgg acgtggactc cgatctgttg
     8221 cgcgcggcgg aggcgcaggg cgagattgtc tggcgtatcc atcgccgcca ccttgccctc
     8281 atggatgatg acgatgcgtc cgcacagcat ctccacctcg ggcaggatgt gggtgctcag
     8341 gatgatcgtg cgatctcccc caaggccctt gatgagctgg cggatctcta cgatctggtt
     8401 tgggtcgagg ccgacagtgg gttcgtcgag gataagcaca tcagggttgt ggatcagcgc
     8461 ctgggccagg ccgacacgct ggcggtagcc cttggagagc ttggagatga gcacctcggc
     8521 gtgatccgtg atgcgcgtgg cctccatcac ctcttcgacg cgcttgttca cctggcgcga
     8581 ttcgacgccc ttcaggcgtg ccgcgtactg caggtaactg cggggcgtca tatcgtcata
     8641 gaggggcacg ttctcgggca gatacccgat gcgcttgcgc gcttcaagcg actctgagac
     8701 cacgtcgaag ccggccacgg cggccttgcc gctggttggc tgcagaaagc cggtgaggat
     8761 ccgcatggtg gtggtcttcc cggcggcgtt ggggccgagg aagcccagga tctcaccctt
     8821 ctctacggcg aaggatacat tctctatggc cgcgtactcg ccgtagtact tggtcaagtt
     8881 ttctacctgg atcaagagag ctccctcctg aactgcggtg tgcgggcgcg ctatcgaaag
     8941 cccccctgct taccagcgag agggggcgcg cggtttcacc ctcacgaatc cgctgaacgc
     9001 aggggacaat cggcgccagc gcgattcgcg gcaaagctgc ctcccgcttt gagactgtcg
     9061 gtgcgcacag tgcccgccgt agcggggaac ccgagagacg aggcagacgt ggggaagtat
     9121 cctacccgcg ggcttgcgat aacccagacg tctctgcctt cagatcccgg tgatgcaagc
     9181 tgtcacgtag gcagaacaac cgtacttttc acaatcttat agcatacggg ctttgccgat
     9241 gtcaagcgca cgggcagggc agctggatgc ctctggctgc cgggggaata gcgccacaat
     9301 ggtccggttg ttcgcacgca tacatagacc cgctacgctc cacggcggtt cccacgcgga
     9361 atcgcacgcg caatcaggcg cggggcccgc gctcggaggt cgctctggcc ggggtgtggg
     9421 gagagagcgc gggtgcccgg ttacccttgg cagtggtaga ggccggcctc ggtgatgaca
     9481 cggctgacgc gctcgtcaga agtgccagca ggcaccgcag gcagaagctg cgcctcgtag
     9541 gcaagaccaa gacggatgca gtcggggcgc accccgtgaa ggaagcggtc atagtagccg
     9601 gcgccgtagc ccaggcgccg cccctggcgg tcgaacgcga ggccgggcat cacgatgagg
     9661 tcgatctgcg cgggctccat cggcgtcgcc tcggccggct ccaggatccc ccagctgcca
     9721 gggaccagct cgtccagcga gtggatccgg catgcctgca tttcccgccg ctcccgatcc
     9781 acgcgaggaa ccaggagcgc gcgcccgctt tccagcgccg ctgtgaggat cgcccgggtg
     9841 ccaacctcac tgcggaagga gacgtaggcg aaaaccacgc gcacgccggt ccattcgggc
     9901 cccgagagaa gccgctgggt gatcgcgtgg tccgcctccg cgcgccatgc agccgggagg
     9961 gcatcgcgga gggcggtcat tcgggcgcgc agctcggctt tggcagcgcg caggcttgaa
    10021 tcaggcatcc ctatccccgg taattggtga actggagggg gagattgaag tccttctccc
    10081 gcacgagctg gatgatcgcc tgcaggtcat ccttcacctt gccactgac
//
LOCUS       DAOSCV010000026        10122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4361_length_10122_cov_3.838681, whole genome shotgun sequence.
ACCESSION   DAOSCV010000026 DAOSCV010000000
VERSION     DAOSCV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4361_length_10122_cov_3.838681"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLY56_01280"
     CDS             complement(<1..478)
                     /locus_tag="PLY56_01280"
                     /inference="COORDINATES: protein motif:HMM:NF027806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20141.1"
                     /translation="MPQGSCKRALPWALLLGATASGLATWTLRQRRERARAPRIDPER
                     LREAMPEREAKAVAYTQIRYATFFLDEALGLGLLLAMQRHERPIRLREWAERHAKSPG
                     LRDALFTSSLLALSSAITLPFSFVSGHLVERAFGLSRQSALSWMADAAKSLAVSLGI"
     gene            778..2118
                     /gene="clpX"
                     /locus_tag="PLY56_01285"
     CDS             778..2118
                     /gene="clpX"
                     /locus_tag="PLY56_01285"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ20142.1"
                     /translation="MPELISCLICGRKFTSPEPLGKMVPGLGYVCAMDWPGFAAGFEV
                     GRTGMLQGDAGLEDPAVAARDRNLQKLERLLQFDVTPREIAAYLEEYVIGQAHAKRAT
                     AVAVYNHHRSRQIRDVEVSKSNMLFIGPTGVGKTEVGRTLARFLDVPFTIADATNFTP
                     AGYVGDDVENMLMGLLMSADGDEQWAAHGVVFIDEIDKTRRTSAGNPSITRDIGGECV
                     QQAMLKILEGTEARLPFFGGRKHPQGKNITINTKDILFIGSGAFEGLPEIVAKLMRQG
                     QTNLGFRAAPRSSAKEADYRVLLEAPYHILQDALLEYGFIPEFVGRFPVIVPFEALER
                     EEYRAIMTRTRHSILRQQIALFALDGVHLEFTEDAIDAIITQAVQMKSGARALRSLVE
                     RAVSKARFELPERGRERVRCIITAESVESGNYRVEPLTAGHEGQDETLQTAGGE"
     gene            complement(2197..2838)
                     /locus_tag="PLY56_01290"
     CDS             complement(2197..2838)
                     /locus_tag="PLY56_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20143.1"
                     /translation="MRQRKEGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCQ
                     SNLKQIGNGLLMYMQDYDETTCGELVGDYWWTDVIQPYTKNTKIQICPSNRWRAGAFR
                     GCGCFASTITYSEQGRTTKAWWSGYGLAGEMRTPRTAAEVVDPSGTLWAGDARCYFIG
                     NRGWEADYGPGDSKGNHSTADCHSDGNNFLFADGHVKWLAKSSPGMYTLADND"
     gene            3264..5597
                     /locus_tag="PLY56_01295"
     CDS             3264..5597
                     /locus_tag="PLY56_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20144.1"
                     /translation="MSSGVRLPTEGEIEEPPHEPLMENPPEAIRALMAQYLDPGETPE
                     VIVAADMTPDGRYGEAWMMVTEKRIVIINPNHGVPEGWHARLADLERIEKQDFVGSSS
                     LRLVGGGGVCELRFSLTNAEKFADVHEIIEALLHQSDEGEGGNGRRPPQILNRETLRK
                     RAEARAREKGICPKCGRTLPRWSRVCPVCLQTKQLLLRLVPFVRPYWLLIVLNLALML
                     FTTSLALAPPYLTKVLLDDVLPRRELRGLFIVLAALLAISVTNAIVTAMRRYLATWLE
                     QRVVLRLRMDLYEHLHRLSLGFYDRQGSGHVIQRIMSDTTTLQTFMATGLEELTEHVM
                     TILVIAVIMLRMDPGLALITLGPVPLIVWGNHWFAHKVHRLYRRAWRQSAKLNSILTD
                     TLPGMRVVKAFGQEGREVNRFVVTNRELQATNLRAARLGRTFYPLMGFLSTLGTMSIW
                     AYGGYLVIQTGELTAGVLIAFIQYLGRFYQPMHQLSHMNDRIQRVAASAERVFELIDT
                     KPDVADADDAIEMPRIEGHVCFEHVSFSYEMGKRVLEDICLEVQPGEMIGLVGHSGAG
                     KSTLINLICRFYDVGEGAIYIDGHDLRRVRLKSLRDQIGVVLQEPYLFRGTIAENIAY
                     GHPHATLEEIVAAAIAANAHEFICQQPDGYDTMVGERGAGLSGGERQRISIARAILRN
                     PRILILDEATASVDTQTEAKIREAIERLVQGRTTFAIAHRLSTLRNANRLVVLEKGRI
                     VEVGTHAELFAKEGGAFRKLWDMQVDMQKQRAEMMAV"
     gene            5599..6117
                     /locus_tag="PLY56_01300"
     CDS             5599..6117
                     /locus_tag="PLY56_01300"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOJ20145.1"
                     /translation="MDIAGTKSILKILDPQAIRLWRDAVGDLHLELRTEEGETVHHER
                     VRPLRAFPLTAPDTYITFFSERNDYLGVLESLDDVDERTEELLRDEMERRYFLPQIIQ
                     IHYLRIHAGIISWRVETDRGPRRFDVRDRDDIRFIPPRRMVIKDVDGNRFEIQDYMEL
                     DDRSLTLLEQLL"
     gene            6594..7973
                     /locus_tag="PLY56_01305"
     CDS             6594..7973
                     /locus_tag="PLY56_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20146.1"
                     /translation="MTQAELIQTVLQVTQPLEHPRGNRLPLYLWSTMGPGTTDDAETE
                     KIIRSLDARGIALLASWNPRNRDATLAEGLRIGAIQKRLGLPAHINANACLYSFFNGD
                     ERTAHVTESGEPFFDSSFGERYKMGCPFALEHRVPAMREQVEFFVRAYKERDIEIAFA
                     FADWEIDGPIEWNGAWDASKRCARCRKEIPRIDDFHSFQTTLRKIRADLQRRVFAEPV
                     KQAFPKALVGNYAVYPNNGKRYWYDYFELEPGEGIPYEMDQKAKVRPWFADEFRLSGY
                     SFAMPVVYTWYRIFHWYDFENTDYRWFYNMLQVGSNAGEHTPAEVPIITFVHWHTTAL
                     PKDAEPVKQFSEEKYQELLWHLLLRGHDTFFSWCPRAETAVEVRLLHQVWAAALEYRD
                     FLDRGTPVTFHVPPKPGPVVSGLRLGNRVLVRRTEFDDTPGPVSLKIDGKTLAVPRAP
                     GRCQVLTLR"
     gene            complement(8201..8566)
                     /locus_tag="PLY56_01310"
     CDS             complement(8201..8566)
                     /locus_tag="PLY56_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20147.1"
                     /translation="MFVCPSDSSPTMSTPWGNKQLSYCANMCFEQFNLLQMAQLTQPA
                     QYAFAWDSESYYSYWYPESDTSLEATSYRPWLSSAKRHMDGSNMLFLDGHSKWITDGK
                     MKQGILDKSLSFNPRIHNW"
     gene            complement(8651..8875)
                     /locus_tag="PLY56_01315"
     CDS             complement(8651..8875)
                     /locus_tag="PLY56_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20148.1"
                     /translation="MTPPSARKGFTLIELLVVIAIIAILAAILFPVFARARENARKTT
                     CLSNLKQIGSGLLMYVQDYDESLPTGCSYW"
     gene            9235..9828
                     /locus_tag="PLY56_01320"
     CDS             9235..9828
                     /locus_tag="PLY56_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20149.1"
                     /translation="MDLYIFASIGIFGSLLIVLSFVLGEIGHIGGGVDLDHDVAINGG
                     VDHDLPASDAPEADVSAGHDAPGPLSVRVLSIFLSAFGLVGAASRLSGLHTSTSAALG
                     VAAGTVLGWAAWRLMAFLWSQGGSSEVRVRDLEGRMGEVTVAIPAVGPGQVACVVDDV
                     RVYQIARSLAGEEIPIGSRVRITEATPEGVLVERLGE"
     gene            9869..>10122
                     /locus_tag="PLY56_01325"
     CDS             9869..>10122
                     /locus_tag="PLY56_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20150.1"
                     /translation="MLSMLGVPVWIAVPVILVLLFLLFAWMIASFYIRVPPNKVAVIY
                     GRGHGKRADGSRKGSRMLLGGGGIKWPVVEQVAYLELNVMT"
BASE COUNT         1780 a         3094 c         3340 g         1908 t
ORIGIN      
        1 cgatccccag cgagaccgcc aggctcttgg cggcatccgc catccacgag agcgcgcttt
       61 gccgcgacag accaaaggca cgttccacca ggtgcccgct cacgaaggag aaggggaggg
      121 tgatcgcgct cgagagtgcc agcaggctgc tggtgaagag ggcatcgcgc agcccagggc
      181 tcttcgcatg gcgttctgcc cactcgcgca aacggatcgg acgttcgtgg cgctgcatcg
      241 ccaggagcag gcccaggccg agcgcctcat ccagaaagaa agtggcgtag cgtatttgcg
      301 tgtaggcgac cgctttcgcc tcccgctccg gcatcgcttc gcgaaggcgc tccgggtcaa
      361 ttcggggagc gcgcgcccgc tcccggcgct ggcgcagcgt ccacgtggcg aggccgctcg
      421 ccgtagctcc cagcagaagc gcccacggca gagcccgctt gcaggatccc tgtggcatgg
      481 cgccctcctc cccgccgttc ccagcatgat ccaggccacg tccctatacc acggatcgcc
      541 gcgacatcct tttgaacgct cgcgtatgcc acgtggtttc gcgattccgg gggggccgga
      601 cgcgccgcat tctgcatgaa aaccgcgcga ttcggatgca tcacctcttg ccccgcgcgc
      661 caccgcgccg gcccccatct gatggagatt cgccctgttc gagcgttccc aacggtagga
      721 ctctctctct agcagaacga accgggttgt gatatattag tgtagttgga ggttatagtg
      781 ccagaactaa tctcgtgtct gatttgtggg agaaaattca cctcgccgga gccgctgggc
      841 aagatggtgc ccggcttggg atatgtgtgt gccatggact ggccgggctt cgcggccggg
      901 tttgaggtgg ggcgcacagg gatgctgcag ggcgatgccg gcctggaaga tcccgcggtg
      961 gcggcgcgtg accggaactt gcagaagctg gagcgcctgc tccagttcga tgtcaccccg
     1021 cgcgagatcg cggcgtacct ggaggagtat gttatcggcc aggcgcatgc caagcgggcg
     1081 acggctgtgg cggtctacaa ccatcatcgc tcgcgtcaga ttcgggacgt cgaggtgagc
     1141 aagagcaaca tgctcttcat cggcccaacc ggagtgggga aaacggaggt ggggcgcacg
     1201 ctcgctcgct tcctcgatgt accctttact atcgcggatg caacgaactt cactccggcc
     1261 ggctatgtcg gtgacgacgt cgagaatatg ctgatgggcc tgctgatgag cgccgacggc
     1321 gacgagcaat gggccgccca cggcgtcgtc tttattgacg agattgacaa gacgcggcgc
     1381 acgagcgcgg gcaacccatc aatcacgcgc gatattggcg gcgagtgcgt gcagcaggcg
     1441 atgctgaaaa tcctggaggg caccgaggcg cggcttccct tcttcggggg gcgcaagcat
     1501 ccccagggga agaacatcac gatcaacacg aaggatatcc ttttcatcgg cagcggcgcc
     1561 ttcgagggcc tgcctgagat cgtggcgaaa ctgatgcgcc agggccagac gaacctcggc
     1621 ttccgtgcgg ctccgcgcag cagtgccaag gaagccgact accgcgtgct tctggaagcg
     1681 ccctaccaca tcttgcagga cgccctcctc gaatacgggt ttatcccgga gttcgtcggg
     1741 cgcttcccgg tgatcgtgcc gttcgaggcg ttggagcgcg aggagtatcg cgcgatcatg
     1801 acgcgcacgc gtcactccat cctgcgccag cagatcgcgc tcttcgccct cgatggggtg
     1861 catctggagt tcaccgagga cgccatcgat gccatcatta cccaggcggt ccagatgaag
     1921 agtggcgcgc gcgccctgcg cagtctcgtg gagcgggcgg tttcgaaggc gcgcttcgag
     1981 cttccggagc ggggccgcga gcgggtccgc tgcatcatca ctgccgagtc ggtggagagt
     2041 ggcaattacc gggtggagcc actcaccgcc ggacatgagg gtcaggacga gaccctgcag
     2101 acggccggcg gcgagtaggt cctcccgcga tgtggggatg atctaagggt agccgtttgc
     2161 gcctgccgcg gcaggcgcaa acggcgactg cgcggatcaa tcgttatcag ccagcgtgta
     2221 catccccggc gaggacttgg ccagccactt tacgtggcca tcagcgaaga ggaagttgtt
     2281 cccatccgaa tggcagtcgg ccgtgctgtg gttacccttg gaatcgccgg gtccgtagtc
     2341 agcctcccag cctctgttgc cgatgaagta gcagcgggca tcccccgccc acagggtgcc
     2401 agagggatcg accacctcgg cggctgtccg aggggtgcgc atctctccgg ccaggccata
     2461 tcccgaccac cacgccttcg tcgttcgccc ttgctcggag taggtgatcg tcgaggcaaa
     2521 gcagccgcac ccgcgaaagg cgccggcgcg ccagcggttg cttgggcaga tctgaatctt
     2581 ggtgttcttc gtgtagggtt ggattacgtc tgtccaccag tagtcgccga ccagctcacc
     2641 acaggtcgtc tcgtcatagt cctgcatgta catcagcaat ccgtttccga tctgtttcag
     2701 attgctctgg caggtggact tgcgcgcgtt ttctcgggcg cgcgcgaaga ccgggaagag
     2761 aatcgctgcc aggatggcga taatcgctat caccaccaac agctcgatga gcgtgaaccc
     2821 ttctttgcgc tgtcgcatgt gacgcctccc tccgtgggca tggccgcaca ctccgcattg
     2881 tgcaatggac gagacgagat gccggccgcg ttcattctag caggccggcc gagtggaagt
     2941 caagcgtgtg ttgaggtgga tgaagctgcc gggcctcccc gtgcggcgag ggccgtgcga
     3001 cggctggatg cctggagcag gggaccgggc gacgatgaag aaccatgagt acgatgcgga
     3061 gcgccgcggg cttggcaggc gcttcttgtg ggtatgatga tgaacagggg ctgttgggta
     3121 tggcctgagt gggagcgagt gatggtcagg cgctccttgg aacggctacc gatggggcgt
     3181 gggttgaccc aggcctttgc caggcgcatt gattgggttc cggccaagtc cttgcccttt
     3241 cggcgagaga aaggccagac ggcatgagta gcggcgttcg cctcccaacg gagggggaga
     3301 tagaagagcc accccatgag ccgttgatgg agaacccgcc cgaggctatc cgggcgctga
     3361 tggcgcaata cctcgatccg ggtgagacgc ctgaagtgat agtggccgcc gatatgaccc
     3421 ctgatgggcg ctacggcgag gcgtggatga tggtgaccga gaagcgcatc gtcatcatca
     3481 acccgaatca cggggttccg gaagggtggc atgctcgcct cgcggatctc gagcgcatcg
     3541 agaagcagga cttcgtgggg agcagctcgc tccgcctggt gggagggggt ggcgtctgcg
     3601 aactgcgctt ctccctgacc aacgcggaga agttcgccga tgtacacgag atcatcgagg
     3661 cactccttca tcagtctgac gagggtgagg gtgggaatgg tcggcgcccc ccgcagatcc
     3721 tgaatcgcga gacgcttcgc aaacgcgcgg aagcacgggc gagggagaag ggaatctgcc
     3781 ccaagtgtgg gcgcacgctc ccgcgctgga gtcgagtctg ccctgtctgc ctgcagacta
     3841 agcagctcct gttgcgtctg gtgcccttcg tccgtcccta ttggctcctc attgtgctga
     3901 acctcgcgct aatgctcttc accacgagct tggccctggc gcccccgtac ctcacgaagg
     3961 tgctgctgga tgatgttctg ccacgtcggg agctgcgcgg gcttttcatc gtgctggcgg
     4021 cgctgctcgc gatcagcgtg accaatgcga tcgtcaccgc catgcgtcgc tacctcgcca
     4081 cctggctgga gcagcgcgtg gtgctccgcc tgcgcatgga tctctacgaa cacctgcacc
     4141 gactctcctt gggcttctac gaccggcagg gcagcgggca cgtcatccag cggatcatgt
     4201 ccgataccac caccctgcag accttcatgg cgaccgggtt ggaggagttg acggagcacg
     4261 tgatgacgat cctcgtcatc gccgtgatca tgcttcggat ggacccaggg ctggcgttga
     4321 tcaccctcgg cccggtgccg ctgatcgtgt ggggcaacca ctggttcgcg cataaagtgc
     4381 accgcctcta ccgccgcgcc tggcggcaat cggcgaagct caactccatt ctcacggata
     4441 cgctccccgg catgcgcgtt gtcaaggcgt tcggccagga ggggcgggag gtgaatcgct
     4501 ttgtcgtcac gaatcgcgag ttgcaggcga cgaacctgcg cgccgcacgc ctcggccgca
     4561 ccttctaccc cctgatgggc ttcctctcca ccctgggcac catgagcatc tgggcctatg
     4621 ggggctacct ggtgatccag acgggcgagc tcaccgcggg cgtactcatc gccttcattc
     4681 agtacctcgg gcgcttctat cagccgatgc accagctcag ccacatgaat gaccgcatcc
     4741 agcgcgtggc ggcctctgcc gagcgcgtct tcgagctgat cgataccaag cccgacgtcg
     4801 ccgatgcaga cgatgctatc gagatgcccc ggattgaggg acatgtgtgc ttcgagcatg
     4861 tctccttctc ctacgagatg ggcaagaggg ttctggagga tatctgcctg gaggtgcagc
     4921 cgggcgagat gatcggtctc gtcggccaca gcggcgccgg caagtctacg ctgatcaacc
     4981 tgatctgccg cttctacgac gtgggcgagg gggccatcta catcgatggg cacgatctcc
     5041 ggcgcgtgcg cctgaagagc ctccgcgatc agatcggcgt ggtgctacag gagccctacc
     5101 tcttccgggg caccatcgcc gagaatatcg cctacggtca tccccacgcc actctggagg
     5161 agatcgtggc cgccgccatt gccgcgaatg cccacgagtt tatctgccag cagccggatg
     5221 gctacgacac catggtcggc gagcgcggcg cgggcctctc tggtggcgag cgccagcgca
     5281 tctccatcgc ccgcgcgatc ctgcgaaacc cgcgcattct gatcctcgat gaggcgaccg
     5341 cctccgtcga tacccagacc gaggcgaaga tccgtgaagc gattgaacgc ctggttcagg
     5401 ggcggaccac cttcgccatt gcccaccgcc tctccaccct gcgcaacgcg aaccgcttgg
     5461 tcgttctgga gaaagggcgg atcgtcgaag taggaacgca tgcggagctg ttcgccaaag
     5521 aggggggcgc gttccgaaag ctgtgggaca tgcaggtcga tatgcaaaag cagcgcgcgg
     5581 agatgatggc ggtctagaat ggatatcgcg ggcaccaagt cgattctcaa gatcctggat
     5641 ccacaggcca tacgtctgtg gcgcgacgcc gtgggggatc ttcacctcga gctgcgtacg
     5701 gaggaggggg agacggtcca ccacgagcgc gtgcgcccgc tacgtgcctt cccgttgacc
     5761 gcccccgata cctacatcac cttctttagc gagcggaacg actacctggg cgtgctggag
     5821 agcctggatg acgtggacga gcgcaccgag gagttgctgc gcgatgagat ggagcggcgc
     5881 tacttcctcc cgcagatcat ccagattcac tacctgcgga ttcatgccgg catcatctcg
     5941 tggcgggtgg agacagaccg cgggccgcgc cgcttcgatg tgcgtgaccg cgacgacatt
     6001 cgcttcattc cgccgcgtcg gatggtgatc aaagacgtgg atggcaaccg ctttgagatt
     6061 caggactata tggagctgga tgaccgcagc ctcacgctcc ttgagcagct cctgtagccc
     6121 ctgcttcgtg ccttccgcgc cgttcccacc taacagggtt ttgttctgga cggtcgcgca
     6181 cctccttgaa gctggtggcc ttgcgttcga gcgacgcggt gacgccggcc acgcgcgcgg
     6241 gtttcagaga gggtacagcc gcacggcgtc gaatccaggc cgtgcagggg tgaggctggg
     6301 cctccatgcc cgagggaagc gggccctgga tgggagcagt gccactgggg cctgggcagg
     6361 ccggcagact caccacgagg agcggcttcg cggcagaagc acgccctcga tgcgagagag
     6421 tattaaggga aaggaggggt cgtccctggc gggcgacagg cgtcttcccc caccgcggat
     6481 gcgtgtgggc ctcggacgtc actaatccta tgagattcct actggttctg gtcgtgggcg
     6541 ccgccctggc catcacgatg gcagggggcg cgaacgctca ggaaggcagg cgcatgacac
     6601 aggcagagct gattcagacg gtcctccagg tgacgcagcc actggagcat ccgaggggca
     6661 accgactccc cctctatctg tggtcgacca tgggcccggg cacgacggac gatgccgaga
     6721 ccgagaagat catccgcagc ctggatgctc ggggcatcgc gctcctcgcc tcgtggaacc
     6781 cccggaatcg ggatgccact ttggcggagg gactgcgcat cggggcgatt cagaagcggc
     6841 tggggctccc ggcacacatc aatgccaacg cctgcctcta ctccttcttc aacggcgacg
     6901 agcggacggc gcatgtgacg gagagcgggg agccgttctt cgattcctcc ttcggcgagc
     6961 gctacaagat gggctgtcct ttcgcccttg agcaccgcgt gccggccatg cgcgagcagg
     7021 tggagttctt tgtgcgcgcc tacaaggagc gtgacatcga gatcgccttt gccttcgccg
     7081 attgggagat cgacggcccc atcgagtgga atggcgcctg ggatgcttcg aagcggtgcg
     7141 cgcgctgccg caaggagatc ccgcggatcg acgatttcca ctccttccag acgacgcttc
     7201 ggaagatacg cgcggatttg cagcggcgcg tctttgccga gccggtgaag caggcgttcc
     7261 ccaaagcgct cgtgggcaac tacgccgtct atccaaacaa cggcaagcgc tattggtacg
     7321 attacttcga gctggagccc ggcgagggca tcccctacga gatggaccag aaggcgaagg
     7381 tccgcccgtg gtttgccgat gagttccggc tttcgggcta ctccttcgct atgccggtcg
     7441 tctacacctg gtaccgcatc ttccactggt acgacttcga gaacaccgac tatcgctggt
     7501 tctacaacat gctccaggtc ggcagcaacg ccggcgagca cacgcccgcg gaagtgccga
     7561 tcatcacgtt cgtccactgg cacactaccg ctcttccgaa ggatgccgag ccggtgaagc
     7621 agttcagcga ggagaagtat caggagctac tctggcacct gctgctccga gggcacgaca
     7681 ccttcttctc ctggtgccct cgcgcggaga cggccgtgga agtgcgcctg ctccatcagg
     7741 tatgggctgc ggcgctggag tatcgcgact tcctggatcg gggcaccccg gtcaccttcc
     7801 acgtgccgcc gaaacccggt cccgtcgtct ccgggctgcg cctgggcaac cgggtgcttg
     7861 tgcgccggac ggagtttgac gacacgcccg gcccggtctc gctgaagatc gatgggaaga
     7921 cgctcgcggt gccccgcgcg cccggccggt gccaggtcct caccttgcgg tgagcatgcg
     7981 gttctagaat agcttgaggg atgcctgcgc gggcatccct cgggtgtttt gccgccccgc
     8041 acgataggtt tcttgtgggt gcggggccat cagaccgccg accttgcccc catccggatg
     8101 gggagcggag atgcctgccc cggattaggg caagcctctt ccgcgggctc ctggcggagc
     8161 gaggcgcggc gcggccacca agcttccgga taggttcgtg ctaccagtta tggatgcggg
     8221 ggttgaagga gagcgacttg tccaggatcc cctgcttcat cttaccgtcg gtgatccact
     8281 tgctatgccc atccaggaag agcatgttcg agccgtccat gtgccgtttg gcggaggaga
     8341 gccacgggcg gtaggaagtg gcttccagac tcgtatcgct ctcgggatac cagtaagagt
     8401 agtaactctc cgagtcccaa gcgaaagcgt actgcgccgg ctgggtgagc tgcgccattt
     8461 gcaggaggtt gaactgctca aagcacatgt tggcgcaata gctcagctgc ttgttgcccc
     8521 agggtgtact catcgtcggg gagctgtcgc tcgggcagac aaacatctgc gtgttcttgg
     8581 agtagggctg gagaagatcg ctccagatct gcggatcggt tcgtgagccg gagccgccgc
     8641 cgcctggcgc ctaccagtag ctacagccag taggcaggct ctcgtcatag tcctggacat
     8701 acattagcag cccggaacca atctgcttga ggttgctgag gcacgtagtc ttgcgcgcat
     8761 tttcccgtgc ccgcgcgaac acggggaaga ggatcgccgc aagaatggcg ataatggcga
     8821 taaccacgag cagctcgata agcgtgaagc ctttgcgtgc ggatggggga gtcatggtcg
     8881 tctcctttcc tgctgttcga ctagcggtag tttagcacag ctcctcccgc cctgtcaacg
     8941 ccgtggggaa gagagccggt caccggcatc tcccgagagg tcctcatacg ccgttcgatt
     9001 ctgccgttga cgctgggggg gatgggtgag tataatgaca gtgtcaccag accggacggg
     9061 catgcatgcc cgagggtctg ctgcccctcg cgcgatcccg cctcgagggc cggatcgtgg
     9121 cgatgggagt gcagggcggc cacggccagt ctgaagttca gatcatgaga tggccggggt
     9181 gtaacgctgc cgtgtatatg cgagtggatg aggcgcccga agggaggaac cgctatggat
     9241 ctctacatct tcgcgagtat cggcatcttc ggctcccttc tcatcgtgct ctccttcgtg
     9301 ctgggcgaga ttggccacat cggtggcggc gtggatctcg accacgatgt ggcgatcaat
     9361 gggggtgttg atcacgatct gccggctagc gacgctccag aagccgatgt cagcgccggc
     9421 cacgatgccc ccggtccgct cagcgtgcgg gtgctctcta tcttcctcag cgccttcggg
     9481 ctggtaggcg ctgccagtcg gctgagtggt ctgcacacaa gcaccagcgc cgccctgggc
     9541 gtggccgcgg gcaccgtgct cggctgggct gcctggcggc tcatggcgtt cctctggtcg
     9601 cagggcggca gcagcgaggt ccgcgttcgc gacctggagg gccgcatggg agaggtgacg
     9661 gtggcaatcc ccgcagtcgg acctggacaa gtggcgtgcg ttgtggatga cgttcgagtt
     9721 taccagatcg ctcgatctct agcgggggag gagatcccca tcgggtcgcg cgtgcgcatc
     9781 acggaggcta cccccgaagg ggtgctcgtg gagcgtttgg gcgagtagcg tccatccgat
     9841 aagaacgagt agcagaggag gctataccat gctgagcatg cttggggtac ccgtctggat
     9901 cgccgtgccg gtgatcttgg ttctgctgtt cctgctgttc gcgtggatga tcgccagctt
     9961 ctacatcagg gtaccaccca acaaggtggc tgtgatctat ggtcgtgggc atgggaagcg
    10021 cgcggatggc tcgcgcaagg gatcgcgaat gctcctgggt ggaggcggga tcaagtggcc
    10081 tgtggtcgag caggtcgcgt atctcgaact caacgtgatg ac
//
LOCUS       DAOSCV010000027        10088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4378_length_10088_cov_3.886973, whole genome shotgun sequence.
ACCESSION   DAOSCV010000027 DAOSCV010000000
VERSION     DAOSCV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4378_length_10088_cov_3.886973"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="uppS"
                     /locus_tag="PLY56_01330"
     CDS             <1..588
                     /gene="uppS"
                     /locus_tag="PLY56_01330"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOJ20151.1"
                     /translation="EAALELGIEVLTLYTFSVENWRRPQEEVEALMLLIEHLIHREIE
                     ALHAGGVRLGVLGRIHELPPSLQEELRRDMEMTAGNKALLLNLAINYGGRAEIVDAAR
                     ALARRVQAGTLDPDDIEEDVISNALYTAGLPDPDLLIRTAGELRVSNYLLWQIAYTEM
                     WVTDVPWPAFSRRLLLEAIADYQRRQRKFGMVLDS"
     gene            585..1754
                     /locus_tag="PLY56_01335"
     CDS             585..1754
                     /locus_tag="PLY56_01335"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ20152.1"
                     /translation="MSRLAVLGSTGSIGTQTLAVAEEFADRLEVTALAARRDSDALFE
                     QVRRFRPRVCCLVDVEAADRLAGRLRAEGIGTEVLSGPDGLVALATASDVDTVVVSVA
                     GNAALQASLAAIGARKRLAIATKEVLVSAGHLVMREAARTGAEVLPVDSEHSAIFQCL
                     QGEKRAALRRILLTASGGPFLDYTREALETVTVADALAHPTWRMGSKITVDSATLMNK
                     GLEVIEAQWLFGADLSQVEVVVHRQSIVHSLVEFADRSVIAQLGYPDMRLPIQYALFY
                     PERLANNLRPLDLASAGALTFESPDTERFPCLALAYEAARAGGSVPCALNAANEVAVE
                     LFLQGRIGFTDIAALNATVMRRHARIADPDLEQILAVDRWAREEAARVAEEGLDQ"
     gene            1754..2809
                     /gene="rseP"
                     /locus_tag="PLY56_01340"
     CDS             1754..2809
                     /gene="rseP"
                     /locus_tag="PLY56_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOJ20153.1"
                     /translation="MSLAGLETLLAFIIVLGLAVLVHELGHFLAAKAFGVRVLEFAFG
                     FPPKLFTLFRRNGTEYNVCALPIGGYVRLAGTEPGEDAGPDGFNSKPPWQRILVYFAG
                     PLMNFILAGVLFVGLGMTVGVPTKGKEVLISAVVPGEPAALAGFREGDRVVAIDDERI
                     TELDTMLKKVKASPNRPILVTVERDGRQMQIEVVPAVKEGGIGQIGVALSDVTYEKTG
                     VIGAIAHGTEQTWRWTRDTVKGIAQVFTDSEARKSVGGPVAIARLAGMAFRDGAITFL
                     TFLAVISVNLGVLNLLPLLVVDGGQIAFLALEWVRGRRLQPSLQVSIQVVGMVIILLF
                     VALLTVRDITNWVGGKF"
     gene            3184..4113
                     /locus_tag="PLY56_01345"
     CDS             3184..4113
                     /locus_tag="PLY56_01345"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ20154.1"
                     /translation="MKATFLPHFLCVAAIAVNLAARPAAAAERVLFDGQPPAPAGITL
                     GGWGSGSATYTGEEQYEGGGVLRIKVRDFYQGARLDFREPIDLTPVLRQANAYLSLTV
                     KLGGSAQAAPAVAPDMGMLAPGMDPGAMPGMAAPQAPVAKVTKLRIVLQGETGSFELY
                     PMHDIEPTDTGWVTVGIPLESLKERIGEGTFRVKRLVLTANAPDTLSVARIQAINDDT
                     EITADATPSQFNEIPVGQPVRFTAEARAGVTPLKLCWDFDASDGVQEEAVGASVEHTF
                     SKPGRYTVTFTAKPADQADKQPRVTTMEIQVVD"
     gene            complement(4392..5372)
                     /locus_tag="PLY56_01350"
     CDS             complement(4392..5372)
                     /locus_tag="PLY56_01350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ20155.1"
                     /translation="MGATPYLSIVIPVYNEEENVRRLQEQVTAAVDPLGIEYEVIYVD
                     DGSRDRSFEILRELASGNPRMRLIRFRRNFGQTPAMAAGFDAARGEVIVPLDADLQND
                     PVDIPRLLAKLEEGYDVVSGWRQKRQDKGLTRRLPSMIANALIARSTGVRIHDYGCTL
                     KAYRASVLKDVRLYGEMHRFIPAWAALEGARVTEMPVTHHPRTAGKSKYGLSRTFRVI
                     LDLMVVRFLGGYGTKPIHLFGGMGFTLCGAGILTGLITLYQKFFEGIKAHRNPLLMLA
                     VFLFLLGVQAIMLGLLAELGIRTYHESQGKPIYRIAEMVNFEKAPAPVDS"
     gene            5675..6970
                     /locus_tag="PLY56_01355"
     CDS             5675..6970
                     /locus_tag="PLY56_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HOJ20156.1"
                     /translation="MATEIIEARGHIIDSLVLPRILDEILNHGGNFELLDVDIGKQRM
                     DPSYARIRVTAPDQKQLKEILAAVRDHGAQVVGEGGITLAQAPADGVFPEGFYVTTSY
                     PTQVRYGEEWIPVERQRMDCGIRFDPERKRAETAKFCQVRAGDWFVTGSTGVRVNPPG
                     RGRERSIFEFMSSEVSSEKPKHAIIRELAQEMREARAAGKKTLLVAGPAVVHTGAVPS
                     VVGLIEAGVIDLLFAGNALAVHDIENALFGTSLGVDLEHALAAERGHEHHIRAINRIR
                     DAGGIRAAVESGILRSGIMHACIRHGVEFVLAGSIRDDGPLPEVITDVIEAQQRMMAL
                     TEGVQIALMAATALHAIATGNILPATTRIVYVDINPGVVTKLADRGSFQTVGLVTDVG
                     LFFGDLLEQVRGPEPAVAARPRMRRSMVAAGNGGRRSRR"
     gene            6967..7761
                     /locus_tag="PLY56_01360"
     CDS             6967..7761
                     /locus_tag="PLY56_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HOJ20157.1"
                     /translation="MTRVLMCPPDYFGVEYEINPWMHRDEPPVLARAGEQWRALRQVL
                     VESAGATVLEITPHPGLPDFVFTANAGLAIGDTFVASRFRFAQRQKEEAYFHQWFAEH
                     GYRVITLPGDLYFEGEGDAFPVGGNLFAGYHFRSDIRSHHAVADLFELRALSLHLRDP
                     RFYHLDTCFCPLDERTLAYYPPAFDAYACQVIEAHFPARIVVSEEEALRFACNAVVIG
                     THVVLNGGCPRFEQDLREYGFQPHSVDVNEFLKAGGAAKCMTLLLR"
     gene            8002..9276
                     /locus_tag="PLY56_01365"
     CDS             8002..9276
                     /locus_tag="PLY56_01365"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOJ20158.1"
                     /translation="MDPETSLAQGMLADAVDIFECCILTWDFLPVVLPAYLLAGAIAA
                     FVPVTKVLRYLGYQAKRSVAYGTAVCSGIVISMCSCNIVPLALSIFRNGAGIGPAFAL
                     LFAGPALNLVTLVWTYQVFGGLFGAWRLFGTAFVALVVGGVMSLLFQREERKRQAEFA
                     ESGGPATLELELPELEKRYPYRSWALMAGLMVILILGAKGLPWALRIPVLLASAGALV
                     WMLNAWFEPDEVKLWLRETWGFLKLTMPILIPAILLIAVAARHVPLEWFTQRADGTTP
                     FFYLGDNSLRSTTLASIFASLMYFPILTEVPFVKAFLKQGMGVAPSLALLMGGPGVST
                     PGALLLARALGWKKMLAYMALQITGTVGVAYAFGRIHGDYQCPCLTGAEQHVNLEWVT
                     AASGIFAVFLLATVFLMWRRSKNSTTGRAEVP"
     gene            9297..>10088
                     /gene="modA"
                     /locus_tag="PLY56_01370"
     CDS             9297..>10088
                     /gene="modA"
                     /locus_tag="PLY56_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ20159.1"
                     /translation="MRRWIVIALLLGAVMPAQAQPQQKGTLHLFIPCGMILPFNHAKA
                     DFEARTGMKLKITYDNAVALMRRIRDKGERPDIFIAPGELELRQLAAEGYVDPASIVT
                     FGTFKMVLVVPKRNRAGVKSFADLAKPQVRRVAIADPELNSVGHYAREALKGLKLWDK
                     VEGKVLTHWHALEAVHYVCNGRVDAGVYYATCPLESAPEKVGNATYRILAEVPRNLYP
                     PVKVQGGVLKEAKNQAAAQQFLAFLKEPATQRKLSQFGIPNYSELH"
BASE COUNT         1712 a         3112 c         3406 g         1858 t
ORIGIN      
        1 gaggccgcgc tggagctggg gatcgaagtc ctgacgctct ataccttctc ggtagagaac
       61 tggcgccgcc cgcaggaaga ggtggaggcg ctgatgctcc tcatcgagca cctgatccac
      121 cgcgagatcg aggcgcttca cgccggcggc gtgcgactcg gagtgctggg ccgcatccac
      181 gagctgcccc cctctctcca ggaggagctg cgccgcgata tggagatgac cgcggggaat
      241 aaggcgctgc ttctcaacct ggctatcaac tacgggggcc gcgccgagat tgtagacgcg
      301 gcccgggcgc tggcgcggcg cgtgcaagct ggcacgttgg accccgatga catcgaagag
      361 gatgtgatca gcaacgcgct ctacacggcg gggctgccgg atcccgatct gctcatccgc
      421 acggcgggtg agctgcgcgt tagcaactac ctcctctggc agattgccta tacggagatg
      481 tgggtcaccg atgtgccctg gcctgccttc tcgcgccggc tgctcctgga agccatcgcc
      541 gactatcagc ggcgccagcg caagttcggc atggtgctcg actcatgagc cggctcgcag
      601 ttctgggatc gacgggatcc atcggcaccc agacgctcgc ggtggccgag gagttcgcgg
      661 accggctgga agtgaccgcc ctggcggctc gccgggatag cgacgcgctt ttcgagcagg
      721 tgcgccgctt ccgtccgcgg gtgtgttgcc tggtggacgt ggaagcggcc gaccggctgg
      781 caggccgcct gcgcgccgaa gggattggca cggaggtgct ctccgggccc gacggattgg
      841 tggccctcgc cacggcgtcc gatgtggata cggtggtggt gagcgtggcg ggaaacgccg
      901 cccttcaagc atcgctcgcc gccatcggcg cccgaaagcg cctggccatc gcgacaaagg
      961 aggtgttggt ctcggcgggg catctcgtga tgcgcgaagc ggcacgcacg ggcgctgagg
     1021 tgctcccggt ggatagcgag cactcggcga tcttccaatg cctgcagggc gagaagcgtg
     1081 cggcgctgcg ccggatcctc ctgacggctt ccggggggcc gttcctggat tacacccggg
     1141 aagcgctcga gacggtcaca gtcgccgatg cgctggcgca tccaacctgg cgcatgggga
     1201 gcaagatcac cgtggactcc gcgacgttga tgaataaggg tctggaggtg atcgaagcgc
     1261 agtggctgtt cggggcggac ctctcccagg tggaggtggt tgtccaccgg cagtccattg
     1321 tgcattcgct tgtggagttt gcagaccggt cggtgatcgc gcagcttggc tatcccgaca
     1381 tgcgcctgcc catacaatat gcgttattct atcctgaacg attggcgaat aacttgcgtc
     1441 cgctcgatct agcgagtgcg ggcgctttga cctttgagag tccggatacg gagcgtttcc
     1501 cgtgtctggc attggcatac gaggctgccc gcgccggagg aagcgtgccc tgcgcgctga
     1561 acgccgccaa cgaggtggcc gtagaactct tcctccaagg ccgtattggt tttactgaca
     1621 tcgccgcgct gaacgcaacg gtgatgcggc ggcatgcccg aattgccgac ccggatctgg
     1681 aacagatcct ggcggtggac cggtgggcac gcgaagaggc ggcacgggtg gctgaggaag
     1741 gcttggatca ataatgagct tggctgggct ggaaacgctg cttgccttta ttatcgtatt
     1801 gggtctggcc gtgctggtgc acgagctggg tcacttcctt gcggccaaag cgtttggagt
     1861 gcgggtgctg gagttcgcgt ttgggttccc gcccaagctc ttcaccctat tcaggcgaaa
     1921 tggcaccgag tacaacgtct gcgctctccc catcggcggc tacgtgcgcc tggcgggcac
     1981 cgagccgggc gaggatgccg gtccggatgg cttcaacagt aagccaccct ggcagcgtat
     2041 cctggtctac tttgccgggc ctttgatgaa cttcatcctg gccggggtgc tcttcgtcgg
     2101 cctggggatg acggtgggcg tgccgaccaa aggcaaagag gtgctcatct ccgccgttgt
     2161 gcccggggag ccggcggcgc ttgcgggttt ccgcgagggc gaccgtgtcg tggccattga
     2221 cgacgagcgg atcaccgagc tcgataccat gctgaagaag gtcaaggcca gccccaatcg
     2281 gccaatcctc gtcaccgtgg agcgggacgg gcgccagatg cagattgagg tggtgcccgc
     2341 ggtcaaagag ggcggcattg gccagatcgg cgtcgcgctc tccgatgtca cctacgagaa
     2401 gacgggcgtg atcggcgcga ttgcccacgg aaccgagcag acgtggcggt ggacgcgcga
     2461 cacggtgaag gggatcgctc aggtcttcac cgatagcgag gcacgaaaga gcgtgggcgg
     2521 tcccgttgcc atcgcgcgcc tggcgggaat ggcgtttcgc gacggggcga tcaccttcct
     2581 cacgttcctg gcggtcatca gcgtgaacct gggcgtgctc aacctcctgc cgttgctggt
     2641 ggtggatgga ggtcagatcg ctttccttgc cctggagtgg gtgcgcgggc gacggttgca
     2701 gccgtcgctg caggtctcca ttcaggtcgt gggaatggtg atcatcctgc tcttcgtggc
     2761 gcttttgacc gtgcgcgata tcaccaactg ggtcgggggc aagttctgag caggcggcca
     2821 cgcgagggct ttaggcaagg tggagaggcc gtgtagacgg cgcctcatct ggcccgccag
     2881 ggatgccgga gcatcggatc gcctcctcgc gcgcgctcgg agcttcagat gccaacggca
     2941 aatcgctgag cagcggatca agccgccctg agtgaccgat caggcaggcc gaaccgcgct
     3001 gccggtctgg accatcccga gggggcgtct ggcgctgggg gatccgccta gcgggttcta
     3061 ccaatgcccg gcagtaggtg gaagtgccga tcctatttgg ccggtcgcgg cgggatgtgc
     3121 ttttgtgggg gcttggggct atgctggacc cgagcccgcg agcggcaggg agaggaatcg
     3181 atcatgaaag ccacgttctt gccacacttc ctttgcgtgg cagccatcgc tgtcaacctg
     3241 gcggcgcgcc cggcggcggc tgccgagcga gttctctttg acggtcagcc ccccgcgccc
     3301 gcgggcatca cccttggcgg ctggggaagc gggtcggcta cctacaccgg cgaggagcaa
     3361 tacgaggggg gcggcgtact gaggatcaag gtgcgcgatt tctaccaggg cgcccggctc
     3421 gatttccgcg agccaattga cctcaccccc gtcctgcgcc aggcaaacgc ctatctttcc
     3481 ctgacggtga agcttggtgg cagcgcgcag gccgcgccgg cggttgcgcc agacatgggc
     3541 atgttggccc ctggcatgga tcccggtgcg atgccgggca tggccgcacc tcaggcccca
     3601 gttgcgaagg tgacgaagct gcggatcgtt ctccagggcg agacaggctc tttcgagctc
     3661 tatcccatgc acgatatcga gccgacggat accggctggg tgacggttgg cattccgctc
     3721 gaatcgctga aggagcggat cggcgagggc acgttccggg tgaaacgtct ggtgctcacc
     3781 gcgaatgcgc ccgataccct cagcgtagcc cggatccagg cgatcaacga cgacacggag
     3841 atcaccgccg acgcgacccc gtcgcagttc aacgagatcc ccgtgggcca gccggtccgc
     3901 ttcaccgccg aagcccgcgc aggcgtcacc ccgctcaaac tgtgctggga ctttgatgcg
     3961 agcgatggcg tgcaggaaga ggcggttggc gcgagcgtcg agcacacgtt cagcaagcct
     4021 gggcgatata cggtgacctt caccgccaag cccgccgatc aggccgataa gcaacctcgg
     4081 gtcacgacca tggagatcca ggtggtggac tagccccctc gggcggctcc accggcatag
     4141 ccgagtgagc accggtccct tgcgcgccgc gtgggaatag atcaccgcgc tgcttgtacg
     4201 cgaagccctg ctgggttagg ctacactccc gggtgccttt gaacgacagg cctgggagag
     4261 ggagcccgaa gggcttagcc gggaagcagc gcggagctta agctccgcgt gcgcgggtgg
     4321 ccctctccat ccgtatccgc cccttcatcc gtggtgtcag aggtagcgcg cctggaggcc
     4381 cgctcctgct ttcaggaatc gacgggggcg ggtgccttct cgaagttgac catctccgcg
     4441 atccggtaga tcggcttccc ctgagactcg tggtaggtgc ggatgccgag ttccgccagc
     4501 aggcccagca taattgcctg cactcccagg agaaagagga agacggccag catcagcagc
     4561 ggattccggt gtgccttgat cccctcgaag aacttctggt agagggtgat caacccggtg
     4621 aggatgcccg ccccgcagag cgtgaacccc atcccgccaa agaggtggat cggcttcgtg
     4681 ccatagccgc cgaggaagcg caccaccatc agatccagga ttacccggaa ggtgcgcgat
     4741 agcccatact tgctcttccc tgcggttcgc gggtggtggg tcaccggcat ctccgtcact
     4801 cgcgcgccct ccagcgccgc ccacgccgga atgaagcggt gcatctcgcc gtagaggcgg
     4861 acatccttga gcacggacgc gcgatacgct ttgagcgtgc atccatagtc atggatgcgc
     4921 acgccggtgg agcgggcgat gagggcgttc gcgatcatcg aggggaggcg gcgggtgagg
     4981 cccttatcct gccgcttctg ccgccatcca ctgacgacat cgtagccttc ttccagcttg
     5041 gccaggagac gagggatatc aaccggatca ttctgcagat cggcatcgag cggcacgatc
     5101 acttcgccac gggccgcatc aaacccggcc gccatcgccg gggtttgccc gaagttgcgc
     5161 cggaagcgta tcaggcgcat gcgcgggttg ccggaggcga gctctcgcag aatctcgaag
     5221 gagcggtctc ggctgccatc atcgacgtag atgacctcat actcaatccc gagagggtcg
     5281 accgccgccg tcacctgctc ttgcaggcga cgaacattct cctcctcgtt gtagaccggt
     5341 atgacgattg ataggtaggg cgtggccccc atgcgtctcc cttcagcgcg cagatgccgt
     5401 agagcaggca aattgctcct accctccgat tcgccggaaa gatctggata ccttccccac
     5461 tatggtggct gcgccggcgg agcagcgagc gcgcatccgc gaggagaacg gccaaccgaa
     5521 gtttcagcac cgagggtgca ggggtaagct agtgcgagcg gttctgaatc tgccggtata
     5581 ggggtgccga tgccggtcga gcggcggcgc cttgcccgcc gtctgcctgg tgattgcggg
     5641 ggcgcggcgg ccagcaaagc aggggagagc agagatggcg acagagatca ttgaggcccg
     5701 cgggcatatc attgattccc tggtcttgcc ccgcatcctg gatgagatcc tcaaccacgg
     5761 gggcaacttc gagctactcg atgtcgatat cggcaagcag cggatggatc ccagctacgc
     5821 gcgaatccgg gtgaccgcgc cggaccagaa gcagctgaag gagatcctcg ctgccgttcg
     5881 ggatcacggc gcgcaggttg tgggagaggg tgggatcacg ctggctcagg cacccgccga
     5941 cggcgtcttt cccgaaggct tctacgtcac caccagctat ccgacgcagg tgcgctacgg
     6001 ggaagagtgg ataccagtgg agcgccagcg gatggattgc gggatccggt tcgacccgga
     6061 aaggaagcgc gcggagacgg ccaagttctg ccaggtgcgc gcgggcgatt ggttcgtcac
     6121 gggaagcacg ggggtgcgcg tgaacccgcc cggccgggga cgcgagcgga gcatctttga
     6181 gttcatgtcc agcgaggtgt cgtccgagaa gccgaagcat gcgatcatcc gcgagctggc
     6241 gcaggagatg cgtgaagcgc gcgctgccgg gaagaagaca ctcctagtcg cggggcccgc
     6301 agtggtgcat accggggcgg ttccttcggt ggtcggcttg atcgaggccg gggtgatcga
     6361 tctcctcttc gcgggcaacg cgctcgcggt gcacgatatc gagaatgctc tctttggcac
     6421 gtcgctgggc gtggatctgg agcacgcgct cgcggcggag cgcgggcacg agcatcacat
     6481 acgcgccatc aaccggatcc gtgacgcggg aggcatccgc gcggcggtag aatccgggat
     6541 cttgcgcagt ggcatcatgc acgcctgcat ccgccacgga gtcgagtttg tcctggcggg
     6601 ctccatccgc gacgatggtc cgcttccgga agtgatcacc gacgtgatcg aggcacagca
     6661 gcggatgatg gcgctcaccg agggagtgca gatcgcgctc atggctgcaa ccgccctcca
     6721 tgccatcgcc accgggaata tcctccccgc caccacgcgc atcgtctatg tggatatcaa
     6781 cccgggcgtg gtgacgaaac tggccgaccg tggcagcttc cagacagttg gtctggtgac
     6841 ggatgtgggg ctcttcttcg gcgatttgct ggaacaagtg agagggcccg agccggctgt
     6901 ggctgctcgc ccgcgaatgc gtcggagcat ggtggctgcg ggtaacggtg gaaggcgatc
     6961 gaggcgatga cccgggtctt gatgtgcccc ccggattatt tcggggtgga gtatgagatc
     7021 aatccgtgga tgcaccgcga cgagccgccg gtgctggcgc gcgcgggtga gcaatggcgc
     7081 gcgctgaggc aggtgctcgt cgagagtgcc ggagcaacgg ttctggagat cacgccgcat
     7141 ccgggcctac cggattttgt ctttaccgcg aatgccggtc tggccatcgg cgataccttc
     7201 gttgccagcc gcttccgctt tgcccagcgc cagaaggaag aggcttactt ccaccagtgg
     7261 ttcgccgagc atggctaccg agtcatcacg ctgcccggag atctctactt cgagggtgaa
     7321 ggcgacgcct tcccggtggg gggcaacctc ttcgccggct accatttccg gtcggatatc
     7381 cgctctcacc acgccgtggc cgatcttttc gagttgcgcg cgctctccct ccacctgcgt
     7441 gacccgcgct tctaccatct ggacacctgt ttctgccctc ttgatgagcg gactctcgcc
     7501 tactaccccc cggccttcga tgcctatgcc tgccaggtga tcgaggcgca ctttccagcc
     7561 cggatcgtcg tgagcgaaga ggaagccctc cgcttcgcgt gcaacgcggt cgtgatcggc
     7621 acgcacgttg ttctgaacgg ggggtgccct cgcttcgagc aagacctccg ggagtatggc
     7681 ttccagcccc actcggtgga tgtgaatgag ttcctgaaag ccggcggcgc ggcaaagtgc
     7741 atgacgctcc tcctgcggta agagcgcgga cctggagaat cggaccggct gcccggagtg
     7801 acgtacgggc gcagtgaacc cggtgggagc gggcccggga ccataccgac cgccagaaac
     7861 acggaacggg gattgactcc gccgtgtctt ctatggtaac gtactctcag gcagcggtaa
     7921 acgagcaggc aggggttccc cttactctcc agcggttgga cgcggccttg cctgcgcagg
     7981 cctcacggaa cggacagttt gatggaccct gagacgagcc tggcacaggg gatgctggca
     8041 gacgctgtag acatcttcga gtgctgcatc ttgacgtggg atttccttcc cgtcgtcctc
     8101 cccgcctacc tgctggctgg cgctatcgcg gcattcgtgc ccgtgaccaa agttctgcgc
     8161 tacctgggct atcaggcgaa gcgctcggtc gcctacggca cggcggtctg ctcggggatc
     8221 gtgatctcga tgtgctcgtg caacatcgtg cccctggcgc tcagcatctt ccgcaacgga
     8281 gcggggatcg gccccgcgtt cgcgctcctc ttcgccggac ccgcgctcaa cctggtcacg
     8341 ctcgtctgga cttaccaggt cttcggggga ctctttgggg catggcgcct ctttggcacc
     8401 gccttcgtag ctctggtcgt cggcggggtg atgtcgcttc ttttccagcg cgaggaacgc
     8461 aagcgccagg cggagtttgc cgagagcggg ggccccgcga cactggagct ggagctgccg
     8521 gagctggaga agcgctatcc ataccgaagc tgggcgctga tggccgggct gatggtcatc
     8581 ctcatcctgg gggccaaggg gcttccgtgg gccctccgga tcccggtgct cctcgccagc
     8641 gcgggcgcgc tcgtgtggat gctgaacgcc tggttcgagc ccgatgaggt gaagctgtgg
     8701 ctgagggaga cgtggggctt ccttaagttg acgatgccca ttctgatccc ggccatcctc
     8761 ctcatcgccg tcgcggcgcg ccatgtgccc ctggagtggt tcacgcagcg tgccgatggc
     8821 accacgccct tcttctatct gggagataac tccctgcgct ccaccacgct cgcctcgatc
     8881 tttgcctcgt tgatgtactt cccgatcttg acggaggtac cttttgtgaa ggcgttcctg
     8941 aagcagggga tgggagtggc gccctcgctg gcgctcttga tgggtggtcc gggtgtgagc
     9001 acccccggcg ccctcctcct ggcacgcgcc ctcggctgga aaaagatgct tgcctatatg
     9061 gcgctgcaga tcaccggcac cgtgggcgtg gcttacgcct ttggccggat tcacggcgac
     9121 taccagtgcc cgtgcctgac tggcgctgag cagcatgtca acctggagtg ggtgaccgcc
     9181 gcatccggca tcttcgcggt gtttctcctc gccaccgtct tcctcatgtg gcggcgcagc
     9241 aagaactcga ccacgggacg agcagaggtc ccctagaaga gataggagcg gaagagatgc
     9301 gtcgttggat cgtaatcgcg ctgctcttgg gagcagtgat gccggcccag gcacagccgc
     9361 aacagaaagg caccctgcat cttttcattc cgtgtggcat gatcctgccg ttcaaccacg
     9421 ccaaggcgga tttcgaggcg cgcaccggga tgaagctcaa gatcacctac gataacgcgg
     9481 tggcgctgat gcggcgtatc cgcgacaagg gcgagcgccc ggatatcttc atcgccccag
     9541 gcgagctgga gctccggcaa ctcgccgccg agggctacgt tgacccggcc tccatcgtca
     9601 ccttcggaac cttcaagatg gtgctggtgg tgcccaagcg caaccgcgcc ggcgtcaaga
     9661 gcttcgcgga tctggccaag ccccaggtgc gccgtgtcgc catcgccgat cccgagttga
     9721 actcagtcgg ccactatgcc cgcgaggcgc taaagggcct gaaactgtgg gacaaggtgg
     9781 agggcaaggt gctgacgcac tggcatgcgc tggaggcggt ccattatgtc tgcaacggcc
     9841 gcgtcgatgc tggcgtctac tatgcgacct gcccgctgga gagtgccccg gaaaaggtgg
     9901 ggaatgctac ctaccgaatc ctggcggagg tgccccgaaa tctctacccg ccggtgaaag
     9961 tccagggcgg cgtgctcaag gaggcgaaga accaggccgc ggcccagcag ttcctcgcct
    10021 tcctgaagga gccggcgact cagcgcaagc tctcgcagtt cggcattccc aactatagcg
    10081 agcttcat
//
LOCUS       DAOSCV010000028         9970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4449_length_9970_cov_3.124962, whole genome shotgun sequence.
ACCESSION   DAOSCV010000028 DAOSCV010000000
VERSION     DAOSCV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4449_length_9970_cov_3.124962"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="PLY56_01375"
     CDS             complement(<1..780)
                     /locus_tag="PLY56_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013605760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOJ20160.1"
                     /translation="MGTPKAKTRQSAAAPAPSPEPPATGANALSELGEKIFLDRYALK
                     DASKKTLAVGDTVVVCTDVKTGQREIGTVTEIQDGSVMVRLRDGTLQQRAIEQIDKPL
                     ETRPEEMMARVARGIAAVEGEHAAEWEEKFRWLLDDWKFVPAGRILAAAGTDQALTYY
                     NCYVIPSPRDSRQGIVKTLANMMEIMSRGGGVGINLSSLRPRHAYVRGVNGRSSGSVS
                     WGALYSFVTGLIEQGGSRRGALMLILNVWHPDVLDFITAKRE"
     gene            complement(960..1460)
                     /gene="nrdR"
                     /locus_tag="PLY56_01380"
     CDS             complement(960..1460)
                     /gene="nrdR"
                     /locus_tag="PLY56_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOJ20161.1"
                     /translation="MKCPYCGGSQDKVIDSREIRDGEATKRRRQCNECGKRFTTYEEI
                     EERRLMVVKKDGRREPYDRGKILRGLQTACHKRNVDADVLMRITEEIDREIINRSDRE
                     VASVEIGEMVMARLRELDHVAYVRYASVYRQFRDVTQFRELVDVLGHEEREQSQQRQP
                     RLPYER"
     gene            complement(1595..2716)
                     /gene="queG"
                     /locus_tag="PLY56_01385"
     CDS             complement(1595..2716)
                     /gene="queG"
                     /locus_tag="PLY56_01385"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOJ20162.1"
                     /translation="MDESAGLSQRLKEHARALGFDHVGIAPARPGPHAHHLDGWLACG
                     YAARMAYLARDPERRKDPRFVLPGAQSVIVCALAYAARDSSTLPADRPRIADFAHGRD
                     YHLVVGEKLRALEAFLHAEAPEAVQSRAYVDTGPVLEREAAFLAGIGRYGKNTTLIHP
                     TLGSRFFIGVVITTALLEHDQALAGDPCGSCTRCLKACPTGALRAPYELDARRCLSYL
                     TIEHRGVLPQEARPALGNRVFGCDACQEACPWNARLPQQPPSPPVDNAPSLLDLLHLP
                     NEEFSRRFGQTPVKRAKWSGLRRNAAIALGNTRDPAAIPALTRALADSDPLLRAHAAW
                     ALGRIGTPEAVAALRAALATETDPAVIAEIQAAQRLAAQ"
     gene            3039..4226
                     /locus_tag="PLY56_01390"
     CDS             3039..4226
                     /locus_tag="PLY56_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20163.1"
                     /translation="MRAGGSASDAAKDESAADAAPPIEPSLLRALQQILDFGRDALHV
                     RAAILVLKLGEGDYSPHFLSSAPEGEAGEWVDAIRAGQGGLDTVLAEGRPVRLHHTAR
                     RSGGGPADRWLRVTSLLGIPMGDGGLFFVEKEDGHHFTDADERLALFLAEQAAAVVEC
                     AHLLAETRSRCDETVRAERLSAISTLASVIGHDLRNPLSVINNAIFFLSSKIESDDAR
                     VNRHLEIVRREIRQATRIIEELLDFSRDRAPQPAPAPLRVLLRDALASRRIDGQIRVE
                     EELPADLPLLYVDVVQVRQALAHLLANAIEAMPEGGTLRVRAFACDAGVAVQIEDTGV
                     GIPSEHRDRLFEPLFTTKPRGAGLGLALVQRVVAAHHGTIRIESEDGQGTTVTLLLPA
                     AER"
     gene            4527..5333
                     /gene="tpiA"
                     /locus_tag="PLY56_01395"
     CDS             4527..5333
                     /gene="tpiA"
                     /locus_tag="PLY56_01395"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ20164.1"
                     /translation="MKRIPIVAGNWKMHKTIAEARALVDGLKPGVAGLTGVEVVVCPP
                     YTALAAVREAIEGSAIALGAQDVFWKEKGAYTSQVAPSMLVDAGCRYVIIGHSENRGR
                     FGVPEPEMTEALLRWFGETDESVNVKTKAALAAGLIPIVCVGETLDERRAGKTDEVIA
                     GQVERGLAGLTAEQVGGMVIAYEPVWAIGTGETCDTPEANRVCGMIRSVVERVFGADA
                     AQAVRIQYGGSVKPGNAKELFSQEHIDGGLVGGAALEAESFTQIVRAALS"
     gene            complement(5581..5919)
                     /locus_tag="PLY56_01400"
     CDS             complement(5581..5919)
                     /locus_tag="PLY56_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20165.1"
                     /translation="MPLAEEPAQQDERASCASWMIAWVLGPVITWVIDVVQALNAGVP
                     SRGLFGSVEAASMGLLSAGGGYFAVYLGIRSLLEGPRLILEQLSGAAVRISPMWTGLL
                     ALLVIFYLDL"
     gene            complement(6058..7173)
                     /locus_tag="PLY56_01405"
     CDS             complement(6058..7173)
                     /locus_tag="PLY56_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HOJ20166.1"
                     /translation="MKRLLFALLLITSSGALGTSFARADALSEIAALPAVSVETIGRS
                     VEGRPLHLLVVAEPEAIERGAPSVLIICGQHGNEPIASQAALRVVRNLCSESKAAIRR
                     RIVTLIVPIANPDGMAVRRRTNAAGVDLNRDWQERTQPETRAIFDVFLQWRPTLIIDA
                     HEWTADDLYTCHTIETPLLRQERAVPTWSVPLDQWISTTLRACADQGTYVQRTYWGAA
                     NDPRLAHRFFTTAWNTPAILYETMPAGTASNATRITAATTFLSQMLAAVAASAPAPSP
                     VPPQSPVPAPDSSMWPIHFALASGLLFLLLFTHGQGGRGMMERTTTRPLSVPRRSTGP
                     IIPRCRRERPLASARTRHFPRRAHAPQDPRFSSLSAN"
     gene            complement(7175..7537)
                     /locus_tag="PLY56_01410"
     CDS             complement(7175..7537)
                     /locus_tag="PLY56_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOJ20167.1"
                     /translation="MTDVIQPVFRRIEPSEAAILHRDTYAGTSPSVYTDRRVAPPPGN
                     GPDLSEVQQAVDCLNEAVKTLEIGARFRILDEPRQVIVEMYDTATGDVIREIPPRRLL
                     RMYADMMHLIGLSLDRKA"
     gene            complement(7804..8967)
                     /locus_tag="PLY56_01415"
     CDS             complement(7804..8967)
                     /locus_tag="PLY56_01415"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ20168.1"
                     /translation="MRICILGAGYAGLTLARELDRWLGPNTPHEILLVDRSCHHQLIT
                     RLHEVAAATIPPDAAQIPFSRALRGSRVHFHQATVTAIDPSTKRVHCDGHEFGFDILV
                     VALGGEAAESGLPGIAENALPLRNCDQAVRLARHVERQVVRARRASDAAIREAHLTAV
                     IIGGGCTGVELAGELHDRLRDLVALHELSESARVLLIESCDQLLPGFPLNAAYRAQEI
                     LAQLGVEVRLLSPVAAVRPGQVTLASGERIAAGTIAWAGGTRAPRLLAESGLPTDHHG
                     RACVDAYLRWSERSDLFAIGDVCHILDHDAGAPVSATAQHAIQQARHLAYSLYRLLQG
                     RSIVRYRPAPAPEILSLGRHHAIAVLGSVVLAGREARLLKHLSYRRYEASVRA"
     gene            9328..>9970
                     /locus_tag="PLY56_01420"
     CDS             9328..>9970
                     /locus_tag="PLY56_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ20169.1"
                     /translation="MAAVVPVTVSAIVLVARGHVPLIGVVDALLVGLMVGAGMALSLV
                     NGLLRSITRLRISTRRIALDSGGDAPPEKGGIDDLDQLAAWLSAVSRHVRRSLREARE
                     REQETEAVFARMADGILLVDGDLRIRRLNPAAAEMLALTPGRALGYTVIEATMLHSLD
                     GLFREAMLAGATRMRRLETIQPRRRLLHVLVTPLEGGVRGSGAVAVLQDLTEFD"
BASE COUNT         1624 a         3147 c         3517 g         1682 t
ORIGIN      
        1 ttcgcgcttg gcggtgatga agtccagcac gtccgggtgc cagacgttga ggatgagcat
       61 cagcgcgccg cgacgcgagc ccccttgctc gatcagcccg gtgacgaagg agtagagcgc
      121 gccccatgag accgacccgg aagagcggcc gttgacgccg cgcacgtagg cgtgccgggg
      181 gcggagactg gaaaggttga tccccacgcc gccgccgcgc gacatgatct ccatcatgtt
      241 ggcgagcgtc ttcacgatcc cctggcggga gtcgcgcggg ctgggaatca cgtagcagtt
      301 gtagtaggtc agcgcctggt ccgtgccggc tgccgccagg atgcgcccgg cgggcacgaa
      361 tttccaatcg tccagcagcc agcgaaactt ctcttcccac tcggcggcgt gctcgccctc
      421 cacggccgcg atcccgcgcg ccacacgggc catcatctcc tcagggcgcg tctcgagcgg
      481 cttgtcgatc tgctcgatgg cgcgctgctg gagcgtgccg tcgcgcaagc gcaccatcac
      541 ggagccgtcc tggatctcgg tgacggtgcc gatctcgcgc tgccccgttt tcacgtcggt
      601 gcagacgacc acggtatcgc ccacggcaag agttttcttg cttgcgtctt tcaaggcata
      661 ccggtcgaga aagatcttct cacccaactc gctcagcgca ttcgccccgg tcgcaggcgg
      721 ctcgggtgag ggggcaggcg cggccgccga ttgccgcgtc ttggctttgg gggttcccat
      781 gggttgctcc ttcattacag cttctcttat cccaggagac ggcggagtca gcggtagctt
      841 cgcgcgcatg gcgggggcgt cgcgagccaa tgcaatgacg tctcctcagg cagagcgcgg
      901 ctcgtgccgc gcgcgctcgt actcctatgt cgtttccacg ccggtcgatg ccggcggaac
      961 tatctctcgt aggggaggcg gggttggcgc tgctgcgact gctctctctc ctcgtggccg
     1021 agaacgtcta ccaactctcg gaactgggtg acgtcgcgaa actgacgata aacggaggca
     1081 tagcgcacat aggcgacgtg gtccagctct cgcagccgcg ccatcaccat ctcgccaatc
     1141 tcgacgctgg cgacctcgcg atccgaccgg ttgatgatct cccggtcgat ctcctcggtg
     1201 atccgcatga gcacgtcggc atcgacgttg cgcttgtggc aggccgtctg gagaccccgc
     1261 aggatcttcc cgcgatcata cggctcccga cggccgtcct tcttgacgac catcaagcgc
     1321 cgctcctcga tctcctcgta ggtggtgaaa cgcttgccgc attcgttgca ctgccgccgg
     1381 cgtttcgttg cctccccgtc tcggatctcg cgggagtcaa tcactttgtc ctgtgaacct
     1441 ccgcagtatg ggcacttcaa cgatgccatc cccctacaag atgtggtatg gtgacccact
     1501 atagcacacc agatatgcgc ctgtcaagga gttcagcggc gattgtgagt gtcaaagagg
     1561 ggcaggaaga gcgacgggct tccgggggcc ggcttcactg cgccgcgagg cgttgtgccg
     1621 cctggatctc tgcgataacg gcgggatccg tctccgtcgc cagggcggcg cgcagagccg
     1681 cgaccgcctc tggggtgcct atccggccga gcgcccaggc ggcgtgcgcg cgcaggaggg
     1741 ggtcggagtc ggcgagggca cgcgtcagcg ctgggatggc ggctggatcg cgcgtgttgc
     1801 cgagggcgat ggcggcgttg cggcgcaggc cgctccattt cgcgcgcttc accggcgttt
     1861 ggccaaagcg gcgcgagaac tcctcgttgg ggagatgcaa caggtcgaga agagaagggg
     1921 cattgtccac gggtggcgaa gggggctgtt ggggcaggcg ggcattccag gggcaggctt
     1981 cctgacaggc gtcgcacccg aagacgcgat tgcctagcgc cggcctagct tcctgtggca
     2041 gcacaccccg gtgctcgatt gtcaggtagg agaggcagcg gcgcgcgtcc agctcatagg
     2101 gggcgcggag ggcgcccgtg gggcacgcct tcaggcagcg cgtgcagctg ccacacgggt
     2161 caccggctag cgcctggtca tgctccagga gcgccgtggt gatcaccacc ccgatgaaga
     2221 agcgcgagcc gagcgtcggg tggatgaggg tggtgttctt gccgtagcgt ccgatgcccg
     2281 ccagaaaggc ggcctctcgc tcgagcaccg gaccggtgtc cacataggcg cgactctgga
     2341 ccgcctcggg cgcctcggcg tggagaaacg cctcgagggc gcgcagcttc tcgccgacaa
     2401 ccaggtgata gtcgcgtccg tgggcgaagt cggcgatgcg cgggcggtct gcggggagcg
     2461 tggacgaatc gcgagcggcg taggcgaggg cgcagacaat caccgattgg gcgcccggga
     2521 gcacgaaacg cgggtccttg cgccgctccg gatcacgcgc cagataggcc atccgggcgg
     2581 catagccgca cgcgagccag ccatccaggt ggtgagcgtg ggggcccggg cgggccggcg
     2641 cgatccccac atgatcaaac ccaagcgcgc gcgcatgttc cttgaggcgc tgtgagaggc
     2701 ctgccgattc atccatcacc ccacgattct accacgacgc tggcgaaatg ggaacccgcc
     2761 ctcgggcgcg tctggccgcc aggggcgcgt gagccaacat catgtttggt cgctggagca
     2821 agcgggtata cctaaagtta acaagcgggt gttcgacagc cgagggcggg cggtgctgcc
     2881 caggcaagcg cgggaccacc catccgggag tatccatgac gatccccaat gccagcaatc
     2941 gcgatggagt gctcgaccaa gagcccggcg tggagtcgag cgcggaccca tgcaaaatgt
     3001 tcggagacct tccgcccctg gaaaggagcc gcccgtcgat gcgcgccggc ggtagtgcga
     3061 gcgacgcggc gaaggatgag tccgccgctg acgcggcccc tccaattgag ccctctctgc
     3121 tgcgggcgct ccagcagatc ctcgacttcg gccgcgacgc gttgcatgtg cgcgcggcca
     3181 ttctggtact gaagctggga gagggagact actccccgca ctttctgtca tccgcgcccg
     3241 agggggaggc gggggaatgg gtggatgcaa tccgcgccgg gcagggcggc ctggacacgg
     3301 tcctggcaga gggccggccc gtgcgcctcc accacacggc gaggcgttcc gggggcggtc
     3361 cggcggatcg atggctgcgt gtgacttcgc tcctgggtat cccgatgggc gacggaggcc
     3421 tcttcttcgt ggagaaggag gacgggcacc acttcacgga tgcagacgag cgcctggcgc
     3481 tcttcctggc ggagcaggca gccgccgtgg tggagtgcgc ccatctgctt gcggagacgc
     3541 gctcgcgctg cgacgagacg gttcgcgcag agcgcctcag cgccatctcc accctcgcgt
     3601 cggtgatcgg gcacgaccta cgcaatccgc tctcggtgat caacaacgcc atcttcttcc
     3661 tcagctcgaa gattgagagc gacgacgcgc gggtgaaccg ccacctggag atcgtccggc
     3721 gtgagattcg ccaggcaacc cggattatcg aggaactcct cgatttctcg cgagaccgcg
     3781 cgccccaacc tgcgccggcg ccgctgcgcg tgctcctccg cgacgcgctc gcatcccgcc
     3841 ggatcgacgg gcagatccgg gtggaagagg agcttccggc ggatttgccc ctcctctacg
     3901 tggatgtggt gcaggtgcgc caggcgctcg cccaccttct ggccaatgcc atcgaggcga
     3961 tgccggaggg cggcacgctc cgggtgcgcg cattcgcttg cgacgccggt gtggctgtcc
     4021 agatcgagga taccggggtc ggcatcccgt ctgagcatcg agaccgtctc tttgagccgc
     4081 ttttcaccac caagcctcgc ggggctggcc tggggctggc cctggtccag cgcgtggtgg
     4141 cagcacacca cggcaccatc cgcatcgaga gcgaggatgg gcagggcacg accgttaccc
     4201 tcctcctgcc ggctgcggag agatgagctc cggcatggcg tgcggacgag taggcgccgg
     4261 cctgaagagc cgtgcgctat tgagtctgtg cgcgacgccc gggggcgatc cccgccgctt
     4321 ccgagcgcaa gttgacagcc gttagggcca gttgctatac tagggcgagg cggcatggtg
     4381 ctgcctcgct tgtggcaagc ggcgccccgc cgagcggtct gaggggcggg gacgtatcgt
     4441 tcctcctatg aaggttcacc ccatcgcccg aaagcggcgg aagcgtcgcc agccgacggc
     4501 tgattcccga gttggaggct ctcctgatga agcgtattcc gatcgttgcg ggcaactgga
     4561 agatgcacaa gacgattgcc gaggcccgtg ccctggtcga tggtctgaag ccgggtgttg
     4621 ctggtctgac cggcgtagag gtcgtggtct gcccgccata cacggcgttg gctgcggttc
     4681 gcgaggcgat tgagggcagc gcgattgccc tgggtgccca ggatgtcttc tggaaggaga
     4741 agggcgccta cacgagccag gtggcgccga gcatgctggt ggatgcgggc tgtcgctacg
     4801 tgatcatcgg ccactccgag aaccgcggtc gctttggcgt gcccgagccg gagatgaccg
     4861 aggccctcct tcggtggttc ggcgagacgg atgagagcgt caacgtgaag acgaaggcgg
     4921 cgctggcggc cgggctgatc ccgatcgtgt gcgtgggcga gacgctcgat gagcgccgcg
     4981 ctggcaagac cgacgaggtg atcgcggggc aggtggagcg cggtctcgcc ggcctcaccg
     5041 cggagcaggt gggcggcatg gtgatcgcct acgagccggt gtgggcgatt ggcacgggcg
     5101 agacgtgcga cacgccggaa gccaaccggg tgtgcggcat gattcgcagc gtcgtcgagc
     5161 gcgttttcgg cgccgacgcc gcgcaggcgg tgcgcattca gtacggcggc agcgtgaagc
     5221 cggggaacgc gaaggagctc ttctcgcaag agcatatcga tggcggtctg gtgggcggtg
     5281 ccgcgctcga ggccgagagc ttcacccaga tcgtgcgcgc ggccctctcc taagcagagt
     5341 ggccgacgga aagacgcctc gcgctcggtg gtggattccg ctgagaggtg acaccccgtc
     5401 aggcgcgtgg attatcagac gctcggagca cacccctccg ggcgtctgat cccggccccg
     5461 ccttcagggc ggtttagggg gattgccttc agttggaggt agccaccagg atgcgcccgg
     5521 ctctcggggg cctagccggg cttgtggccg atggggcgcg gagtgcccgc atgggcgggc
     5581 tcagaggtcc aaatagaaga tgacgagcag cgcgagcagg cccgtccaca tcggcgagat
     5641 acgcacggcc gcgccgctga gctgttccag aatgaggcga ggtccctcca gcaacgagcg
     5701 gatccccagg tacacggcga agtaaccccc ccccgcgctc aagaggccca tcgatgccgc
     5761 ttccaccgac ccaaacagtc cacgcgacgg tactccggcg ttcagggcct gcacaacatc
     5821 tatcacccag gtgatcaccg gtcccagcac ccaggcgatc atccaactcg cgcacgaggc
     5881 gcgctcatct tgctgcgcgg gctcctcggc aagtggcatt ctcctccctc cgcgttgctc
     5941 acgccgtgcg cgcccggccc gctgggcgtg aaagggaacg gagccaggtc ggcgacgatc
     6001 cccccggcac ctattacccg gtagtgcgga tgggtaacgt gcggacggag ttgttcttca
     6061 atttgccgac aggctggaga agcgcgggtc ttgcggcgcg tgggcgcggc gcgggaagtg
     6121 gcgggtgcgc gcggaggcga ggggacgttc ccgtcggcag cgggggatga tcggcccggt
     6181 ggagcgacgc ggaacgctga gcgggcgcgt cgtcgtgcgc tccatcatcc ccctgcctcc
     6241 ctgaccatgc gtgaagagaa ggagaaacag gagccctgaa gcgagggcaa agtggatggg
     6301 ccacatcgat gagtctggcg ccggaacagg actctgtggc ggaacggggg atggcgccgg
     6361 cgccgatgcg gccacggcgg cgagcatctg agacaggaat gtcgtggccg cggtgatgcg
     6421 ggttgcgttg gatgcagtgc ccgcgggcat cgtctcgtac aggatggcgg gcgtgttcca
     6481 ggcggttgtg aagaagcggt gcgcgagacg cgggtcgttg gccgctcccc agtacgttcg
     6541 ctgcacatag gttccctgat cggcgcacgc tctcagcgtc gtggagatcc actggtccag
     6601 gggaacgctc caggtgggta cagcgcgttc ttgcctgaga agcggcgttt cgatggtgtg
     6661 gcatgtgtag agatcatccg cggtccattc gtgcgcatcg atgatcaggg tggggcgcca
     6721 ttggagaaat acgtcgaaga tggcgcgtgt ctccggttgc gtgcgctctt gccaatcgcg
     6781 gttgagatcg acgccggcgg cattggtgcg ccggcggacc gccatgccat cggggttggc
     6841 aatgggaacg atcagggtga cgatgcgccg cctgatcgcc gccttgctct cgctacatag
     6901 gttgcggacc acgcgcagag cagcttgaga ggcaatgggc tcattgccat gctggccgca
     6961 gatgatcagc acgctgggcg cgccgcgctc tatcgcctct ggttccgcca cgaccaacag
     7021 gtgaagcggc cgcccttcga cactgcgccc aattgtctcc accgagacgg caggcagcgc
     7081 cgcaatctcg gagagcgcgt ccgccctggc gaagctagtc cccagggcgc cggacgaagt
     7141 gatgagcagc agtgcgaaga gtagacgctt cacctcatgc ctttcggtcg agagaaagtc
     7201 cgatcaggtg catcatatcc gcgtacatcc gaagcaggcg acgcggcggg atctcgcgga
     7261 tcacgtcacc ggtggcggta tcgtacatct cgacgattac ctggcgcggc tcatccagga
     7321 tccgaaagcg tgcaccgatc tcaagcgtct tcacggcctc attcaggcag tcgacagcct
     7381 gctgtacctc ggagagatcc gggccgtttc ccggcggcgg ggcaactctc ctgtcagtgt
     7441 acacgctggg cgaggtgcca gcgtaggtgt ctcggtgcag gatggcagcc tcggaaggct
     7501 cgatcctgcg gaacacgggc tgaatgacat cggtcattct tctcaccccc ctcgtccatc
     7561 gaacacacgc gcgaacgggc gcattgcgct atcgctcgct ctcatgggga tcaggcgtct
     7621 cgccaaccgg cggagtgccc tcgccccgat gaacccgcat ctgtagccgc cgcccggatg
     7681 tggtccgctg agtgatggat ccccggaggc gtaccacaag cagggatctt cgcagtgtat
     7741 ctgttcggca ttcgcgtggg ggagcttaag cgcgtcgcga tgtggttcgg ctggcgcgat
     7801 tcctcaggcc ctgaccgatg cctcgtagcg ccgataactg aggtgtttca gaagccgggc
     7861 ctctctccct gccagcacga cggagccaag caccgcgatg gcatggtggc ggcccaggga
     7921 gaggatctcc ggggcgggcg cggggcgata gcgaacgatc gagcgccctt gaagaagccg
     7981 gtagaggctg taggcgaggt gcctggcctg ttggatggcg tgctgtgctg tggcagatac
     8041 cggcgcgcct gcgtcgtggt cgaggatgtg gcatacgtcg ccgatggcga agagatccga
     8101 gcgctcggac cagcgcaggt aggcatccac acaggcccgg ccgtggtgat ccgtcggcag
     8161 cccgctctcg gccaggagac ggggggcgcg ggtgcccccg gcccatgcga tcgttcccgc
     8221 cgcgatccgc tctcctgatg cgagcgtgac ctgacccgga cgaacggcag cgacgggaga
     8281 gagcaggcgc acttcgacgc ccagttgggc cagtatctcc tgcgcccggt aggcggcgtt
     8341 gagggggaag ccgggcagga gctggtcgca tgactcgatg agcaggacgc gagcgctttc
     8401 agagagctca tgcagggcca ccaggtcgcg caggcggtcg tggagttcgc cggccagttc
     8461 gaccccggtg cagccacctc caatgatcac cgccgtcagg tgcgcctccc gaatcgccgc
     8521 gtcggacgcg cgtcgggcac ggactacctg ccgctccaca tgccgcgcca ggcgtaccgc
     8581 ctgatcgcag ttgcgcagcg gcagcgcatt ctcggcaatc ccgggaagcc cgctctccgc
     8641 cgcctctcct cccagcgcca cgaccaggat gtcgaagccg aactcgtgcc cgtcacaatg
     8701 aacgcgcttt gtggacggat cgatggcggt gacggtcgcc tgatggaagt gaacccggct
     8761 gccccgcagc gcgcgagaga aggggatctg cgccgcgtcc ggcggaatag tcgccgccgc
     8821 gacctcgtgt aaccgggtga tgagctgatg gtggcacgag cggtcgacca gcagaatctc
     8881 atggggtgtg ttggggccca gccagcggtc gagctcccga gccagggtga gaccggcata
     8941 gccggcacca agaatgcaga ttcgcatggc tactccacca accggatcac cttctgccag
     9001 ccgcttgtct gggttccagt atagcacccc ggtggggagc ggtcaaggcc gacaacacca
     9061 cgaatgaagg cgacggacgc ggatgggaat gtgcgcgccc cgccggatcc gttgagcgcc
     9121 ctcctcgccg tcggaacgtg ccttcgccgc gcatgaccca gggccttcct gggtatgagc
     9181 atcgcctatc tctgtgaccc gaaggagact tgcgggtggc cactcggatg ggctatactg
     9241 ggaggcagat tgcagcggag gaacgttcgg ccgggagctt ggggaagggt ggtggcccga
     9301 cagatggggt ttcgagcgag agtgctgatg gcagcggtgg tgccggtgac ggtctccgcg
     9361 atcgtgctgg tggcgcgcgg gcatgtgccg ttgatcggcg tggtcgatgc gctccttgtc
     9421 ggcctgatgg tgggcgcggg catggcgctc tcgctggtga atgggctgct ccgatccatc
     9481 acccgcctgc gtatctccac acgccggatc gccctggaca gcgggggaga tgcgcccccg
     9541 gaaaaggggg gcatcgatga cctggatcag ctggcggcgt ggctgtcggc tgtctcccgt
     9601 cacgttcggc gctcgctacg cgaggcacga gagcgcgagc aagagacgga ggcggtcttc
     9661 gcgcgcatgg cggatggcat cctcctggtg gatggcgatc tgcgcatccg ccgcctcaac
     9721 ccggccgctg cggagatgct ggcgctcact ccgggccgtg cgctgggata caccgtgatc
     9781 gaggcgacca tgcttcactc gctggatggt ctgtttcgcg aggcgatgct tgccggggcg
     9841 acccggatgc ggcgcctgga gacgatccag ccccgccggc gcctgctcca tgtgctggtc
     9901 acgcccctcg aaggtggcgt gcggggatcc ggcgccgtcg cggtgcttca ggacctcacc
     9961 gagtttgacc
//
LOCUS       DAOSCV010000029         9756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4568_length_9756_cov_3.638388, whole genome shotgun sequence.
ACCESSION   DAOSCV010000029 DAOSCV010000000
VERSION     DAOSCV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_9756_cov_3.638388"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLY56_01425"
     CDS             <1..264
                     /locus_tag="PLY56_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ20170.1"
                     /translation="TTVPKGKHTIMLCLGTACDVKGAERILQRLKKELGVELGQTTSD
                     HLFTLSAARCVGACGLAPVAMIGDDVYGKLTPDKMIRTLKKYR"
     gene            268..804
                     /locus_tag="PLY56_01430"
     CDS             268..804
                     /locus_tag="PLY56_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20171.1"
                     /translation="MDEIALHVLDIGMNAVAAGARHVVVTIVEDVEKDRLEIRVVDDG
                     KGMDPEQVRDVLERFSSDKAQRKRPIALGLALLRQTAEKCGGGLRITSTPGRGTQVDA
                     WMRHRDYDRPPLGNLADTLFALCLGAPEVDVQCTHQKNGTVFHFDSAQVRQALGPGVS
                     LQSPEGVRAVRRALGSRE"
     gene            801..1169
                     /locus_tag="PLY56_01435"
     CDS             801..1169
                     /locus_tag="PLY56_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ20172.1"
                     /translation="MKLSDLQQWRDRAVRDVSMRGERKEWRIVVSMGTSGIAAGARDV
                     MRALMDEIERQELENVEVALTGSLGLDDFEPVLRVEHQDESVFYGRLTPEAARSIISE
                     HVCQGRKVERFVIGLPKERE"
     gene            1170..2966
                     /gene="nuoF"
                     /locus_tag="PLY56_01440"
     CDS             1170..2966
                     /gene="nuoF"
                     /locus_tag="PLY56_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOJ20173.1"
                     /translation="MPAYRSHVLLCAGGACISCGCLAVRDALEQELQRQGLSDEIRVV
                     QTGCVGSCDLGPVMIVYPDEVFYQKVRPADVPKLVEEHFLKGRIYEPLLVKSPETDNL
                     IATQREFAFFNKQFKIVLENCGIIDPEKIDEYVARGGYQALGMVLTERSPQWVIEEVK
                     KSGLRGRGGAGFPTGIKWELMAKSPGDEKYIVCNADEGDPGAFMDRSVLEGDPHRVLE
                     AMAIAGYAVGARKGFIYCRAEYPLAIKRTQLAINQAREAGFLGKNLFETDFSFDIEIR
                     VGAGAFVCGEETALLASIEGKRGIPRPRPPFPAVSGLWGKPTLISNVETYACICPIIM
                     RGGEWYASIGTEKSKGTKVFALAGKIQNTGLVEVPMGTTLREIVYDIGGGVPNGKKFK
                     AAQTGGPSGGCIPAEHIDVSMDYESLAKMGSIVGSGGLIVMDEDSCMVDVAKFFMDFC
                     MDESCGKCPPCRVGTRQIYGILDKISKGQAVVEDLTLLEELCMMVKETSLCGLGQTAP
                     NPVLSTLRYFRDEYLAHIVDKRCPAKVCPDLVRYTIDEEVCTGCSLCAKNCPENTIFK
                     VDGEKKYRIDPVRCIRCGVCYDVCRFNAVIKE"
     gene            3062..5320
                     /locus_tag="PLY56_01445"
     CDS             3062..5320
                     /locus_tag="PLY56_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ20174.1"
                     /translation="MSDITMTIDGREVRAKQGQTVLEVCREHGIYLPTLCHWPGLSNV
                     GACRLCVVEVEGTNKLGTSCTMPAADGLKIRTQSEALTARRKMTLELLFSERNHFCPV
                     CPESGACELQDLGYRFGIDSIRYPYLFPALSLDVSHDYIAMEPSRCVLCQRCVRICSE
                     RVGVSTLDFRERGIQQMISPDMGLPLGESSCVSCGACMQVCPTGAIFAKKTAYLERAA
                     TAGGPQSAMRPVASTCAGCSLGCRTLVQVSNDRVVRIDADMEAGDAGILCRKGRFMQM
                     EFAGERITQPLVREGDTLKPTDWDTALATVADRLGKIVRERGASAVAGIASPALTNEV
                     LYLFQKVMRAGVGTGNIDAIDGRDLRVSRAATEGLLGPDVRAKVEVRLADLDEADLFV
                     VIGADPTRTHPVAAAAIRRGVYHRRAKLIVINPRKTGLTDLADLAVQPKRGTDGTLLN
                     GVMRALVEAKGGAPAALTAMLMQYTPEAVAAETGVSTDELQQLAEAYTAARHPVLIYG
                     RGITKQNDPRLIATMQALTLVAGHATATSFPILGLRRSASSTGAFDLGVDPTRLPGRL
                     RFTPAEANRFASAWGKPVPEPTSFNANEFDPKTIGALYLLIGDDTLPQEEEMAERLAE
                     LEFVVVQATYRTPLTEVADVVLPAPTWAEQSGTFTGIDGQVRPLRQAVTPPEGVRMPW
                     DVLCSLGNALGLKGSYANVAEVAHEIGRLVPEYAEALATGERAVHFRDASFDVRRSEL
                     RAPDYLAESGGR"
     gene            5323..5862
                     /locus_tag="PLY56_01450"
     CDS             5323..5862
                     /locus_tag="PLY56_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOJ20175.1"
                     /translation="MSDKPKIATVWLGGCSGCHMSLLDLDEGLLDLAKAATITASPIT
                     DIKEPPEVDVCLVEGAVCNTDNLEVLRKCRERAKILVAFGDCACFGNIPAMRNFISLE
                     ETLQRGYVDTESTASGEVPRGEELPALLEKVQPISYYVKVDASLPGCPPSAEAILFAV
                     KELLSGRLPKPEGELLRYD"
     gene            6018..7409
                     /locus_tag="PLY56_01455"
     CDS             6018..7409
                     /locus_tag="PLY56_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ20176.1"
                     /translation="MSRTVTIEPVTRIEGHAKVTVRLGDDGKVQQARMHVVEFRGFEK
                     FCEGRHFTEMPTITERICGICPVSHHLASAKAGDAILGVTIPRTAYMLRELMHMGQIL
                     QSHALSFFHLSAPDLLLGFDADPAVRNVVGLIQQQPEIALKGIKLRKFGQEIIARVAG
                     RKIHPTFCVPGGVNQRLAASDREAMLAGIDEAIAGCQDAISLIKRYYADHADEVARFG
                     NFPSLYAGLVTEDGSLEHYDGRMRFVDADGKVLENGIDPSNYLDYIAETTEEWSYLKF
                     PYFKRVGYPEGAYRVGPLGRLNVADSARTPLANEELNSFKALGDGRPVQGTLYYHYAR
                     MIEALYAVERARQLLEDPEIMGRDIVATSNSLQPRGVGVIEAPRGTLFHDYHVDETGR
                     LEKVNLIVSTGQNNVAMNEAVHPLAGMLNRVEAAIRAYDPCLSCSTHAIGQMPIVVEL
                     IGPDGEQLDRATR"
     gene            7591..8106
                     /locus_tag="PLY56_01460"
     CDS             7591..8106
                     /locus_tag="PLY56_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOJ20177.1"
                     /translation="MLIVGCGNASRRDDGVGLYVIRGLARAFGLPLDEGADEVSVACE
                     VETPDGPVRLELRFEQQLDIVLGEELKDVDLFAVIDAHTGVYPEALRRVEAEPGYTSS
                     LTSHHLTADTLAGLSQALHGRAPRTFIFSVLGHDFNFGEDLTPQTQAAADEAVRQLRD
                     LVQEAARAARA"
     gene            8188..8922
                     /locus_tag="PLY56_01465"
     CDS             8188..8922
                     /locus_tag="PLY56_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ20178.1"
                     /translation="MALLLVTFAVGLLAGGIASVAGFGIGSLLTPLLSVPLGTRLAVA
                     AVSIPHAAGTAVRLWRLHDQVNRRVLWSFGLASAAGGLAGAIGNAVIATGFLTLVLAG
                     LLVLAGSMELTGLSSRMRFAGTAAWIAGGLSGAFGGLVGNQGGIRTAALLGFDLQRDA
                     FVATATAIALMVDLVRVPVYLATQGTAIAAQWPVVTAATAGVLIGTHFGTRVLQRIPE
                     RLFRTLVSLLILALGIALGVRGIRML"
     gene            complement(8940..>9756)
                     /locus_tag="PLY56_01470"
     CDS             complement(8940..>9756)
                     /locus_tag="PLY56_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOJ20179.1"
                     /translation="TDLAPPTERLPHGALYPAAGERRARVAFLTGCVAEALLHRTNRR
                     TVQLLRACGFEVVIPKGQTCCGALHAHAGELEMARRLAKQNIAAFEASGAEFYVSNAG
                     GCGAQLLEYPHLLRDDPQWAERAGRFAARVRDVAELIATQGALPALRRVNERVTYQDS
                     CHLANVQKVKTEPRDLLRRVPGLELVEMTESDRCCGSAGSYSLLHFDVSMRILDEKME
                     QLRRTGATVVTTGNPGCLLQLRLGIHRAGLAGQVRAMHTVDLLAEACEKLPKL"
BASE COUNT         1617 a         3079 c         3409 g         1651 t
ORIGIN      
        1 acgacggtgc ccaagggcaa gcacacgatc atgctctgtt tgggcacagc gtgcgacgtg
       61 aagggcgcgg agcgcatctt gcagcgcctc aagaaggaat tgggcgtgga gttgggtcaa
      121 accacttcgg accacctctt caccctgtcg gcagcgcggt gcgtcggcgc gtgcggtctg
      181 gccccggtag cgatgattgg cgacgacgtc tacggcaagc tgacgccaga caagatgatc
      241 cgcaccctca agaagtaccg ctaggggatg gacgagatcg ccctgcacgt gctagacatc
      301 ggcatgaatg ccgtcgcggc gggggcccgc cacgtggtgg tcaccatcgt cgaggatgtc
      361 gagaaggacc gactggagat ccgcgtcgtc gatgacggca aggggatgga tccagagcag
      421 gtgcgcgacg tgctagagcg cttttccagc gacaaggcgc agaggaagcg ccccatcgca
      481 ctcggcctgg cgctcctgcg tcagaccgcc gaaaagtgtg gcgggggctt gcggatcacc
      541 tcgacgccgg gtcgcggcac ccaagtggat gcctggatgc ggcatcgcga ctatgatcgg
      601 ccaccgcttg gaaacctggc cgacacgctc tttgcgctgt gccttggtgc cccggaggtg
      661 gatgtgcagt gcacccacca aaagaacggc acggtatttc acttcgacag cgcgcaggtg
      721 cgccaggcac tgggtccagg ggtgtccctt cagagtccgg aaggcgttcg cgccgtgcgt
      781 cgggcgctcg ggagcagaga atgaagctat cggatttgca gcaatggcga gaccgcgcgg
      841 tgcgtgacgt gagcatgcgc ggagaacgca aggaatggcg catcgtggtg agcatgggca
      901 ccagcgggat cgcagctggc gcgcgtgatg tgatgcgggc gctcatggat gagatcgagc
      961 gccaggagtt ggagaacgtc gaggtggcgc tcaccggcag cctgggcctg gatgatttcg
     1021 agcccgtgct gcgggtcgag caccaggacg agagcgtgtt ctacgggcgt ctgacaccgg
     1081 aggcggcacg gagcatcatc agcgagcatg tctgccaggg ccgcaaggta gagcgcttcg
     1141 tcatcggttt gccgaaggag agggagtaaa tgcccgccta tcgttcacat gtgctgctgt
     1201 gcgccggagg cgcctgcatc tcgtgcggat gtctcgccgt gcgcgatgcc ctggagcagg
     1261 aactgcagcg gcagggcctg tcggatgaga tccgcgtcgt ccagacgggg tgtgttggct
     1321 cctgcgacct cggtccggtg atgatcgtct atcctgacga ggttttctac caaaaggtgc
     1381 gcccggccga tgttccgaag ctcgtcgagg agcatttcct gaaggggcgg atctacgagc
     1441 ccctgttggt gaagtcgcct gaaaccgata acctgatcgc tacgcagcgc gagttcgcct
     1501 tcttcaacaa gcagttcaag atcgtgctgg agaactgcgg gatcatcgac ccggagaaga
     1561 tcgacgagta tgtggcccgc ggcggctacc aggcgctcgg catggtcctc acggagcgct
     1621 ctccccagtg ggtgatcgag gaggtcaaga agagcggcct gcgcgggagg ggcggcgccg
     1681 gcttccccac cgggatcaag tgggagctga tggccaagtc gcccggtgac gagaagtaca
     1741 tcgtctgcaa cgccgacgag ggcgacccgg gcgcgtttat ggaccgcagc gtgctcgagg
     1801 gcgacccgca ccgcgtgctc gaggcgatgg cgattgccgg atacgccgtt ggcgcgcgca
     1861 agggcttcat ctactgccgc gccgagtacc cgctggcgat caagcgcacc cagctcgcga
     1921 tcaaccaggc gcgcgaggcc ggcttcctcg ggaagaacct cttcgagacg gatttctcct
     1981 tcgatatcga gatccgcgtg ggggccggcg ccttcgtgtg tggcgaggag acggcgctgc
     2041 tcgcgtcgat tgagggcaag cgcggtatcc cgcggccccg cccgcccttc cccgctgtct
     2101 ccggcctgtg gggcaagccg acgctcatca gcaacgtcga gacctacgcc tgtatctgcc
     2161 cgatcatcat gcgcggcggc gagtggtacg ccagcatcgg cacggagaag agcaagggga
     2221 ccaaggtctt tgctctggct ggcaagatcc agaacaccgg cctggtggag gtgccgatgg
     2281 gcaccacctt gcgcgagatt gtttacgaca ttggcggtgg cgtgcccaac ggcaagaagt
     2341 tcaaggcggc ccagacggga ggcccctccg gcgggtgtat cccggccgag catatcgatg
     2401 tctcgatgga ctatgagagc ctggccaaga tgggctccat cgtcggctcg ggcggcctga
     2461 tcgtcatgga cgaagactcg tgcatggtgg acgtggccaa gttcttcatg gacttctgca
     2521 tggacgagtc gtgcgggaag tgcccgccct gccgcgttgg cacgcgccag atctacggca
     2581 ttctcgacaa aatctctaag ggccaggccg tggtggaaga cctcacgctc ctcgaagagc
     2641 tgtgcatgat ggtcaaggag acctcactct gcggcttggg acagacggcg cccaacccgg
     2701 tgctctctac gctgcgctac ttccgcgacg agtacctcgc gcacatcgtg gacaagcgct
     2761 gcccggccaa ggtctgcccg gatctggtgc gctacaccat cgatgaggag gtctgcaccg
     2821 ggtgtagcct gtgcgccaag aactgtccgg aaaacaccat cttcaaggta gatggcgaga
     2881 agaaataccg catcgacccg gtccggtgca tccgatgtgg cgtctgctat gacgtgtgcc
     2941 ggttcaacgc ggtgatcaag gagtagaggt ggcagatggt gggcgacggg ttgcggcaag
     3001 cgggcgcgct cccgcgccgc aagcacgagt ctgcaatctg cagtggcatt gagaggatgc
     3061 gatgagcgac atcacaatga ccatcgatgg ccgcgaagta cgggcgaagc agggccagac
     3121 ggtgctcgag gtgtgccgag agcacggcat ctatctgccc acgctctgcc actggccggg
     3181 gctgtcgaac gtgggcgcgt gccgcctctg cgtggtggaa gtggaaggga ccaacaagct
     3241 gggcacctcc tgcaccatgc cggcggcgga cggcctgaaa atccgcacac aatctgaggc
     3301 gctgactgcg cgccggaaga tgaccctgga gctgctcttc tccgagcgga accacttctg
     3361 cccggtctgt ccggagagcg gcgcctgcga gcttcaggat ctgggctacc gcttcggtat
     3421 cgactccatc cggtatccct atctcttccc cgcgttgtcg ctggatgtct cgcacgatta
     3481 catcgccatg gagcccagcc gctgcgtcct ctgccagcgc tgcgtgcgca tctgcagcga
     3541 gcgggtgggc gtgagcacgc tcgatttccg cgagcgtggc atccagcaga tgatctcgcc
     3601 ggatatgggc ctgcccctgg gcgaatcgag ctgcgtctct tgcggcgctt gcatgcaggt
     3661 gtgccccacg ggcgccatct tcgccaagaa gaccgcctat ctggagcgcg cggcgaccgc
     3721 gggaggaccc cagagcgcga tgcgccccgt cgcgagcacc tgtgccgggt gcagccttgg
     3781 ctgccggacg ctcgtgcagg tctccaacga ccgcgtggtg cgtatcgatg ccgacatgga
     3841 agcaggcgac gccgggatcc tctgccgcaa ggggcgattc atgcagatgg agttcgcggg
     3901 cgagcggatc acccagccgc tggtgcgcga aggcgatacg ctgaagccga ccgattggga
     3961 cacggcgctt gccaccgtgg cagaccgcct gggcaagatc gtccgcgagc ggggcgccag
     4021 cgcggtggcc ggcatcgcct ctcccgcgct cacgaatgag gtgctctacc tcttccagaa
     4081 ggtgatgcgc gcgggagtgg gcactgggaa catcgatgcc attgatggcc gcgatctgcg
     4141 cgtcagccgg gccgcgaccg agggcctgtt gggcccggac gtgcgcgcca aagtggaagt
     4201 gcgcctggca gatctcgacg aggcggatct cttcgtggtc atcggtgcgg acccgacgag
     4261 aacgcacccg gtggcggccg ccgcgatacg ccgcggcgtc tatcaccgcc gggcgaagct
     4321 gatcgtgatc aacccgcgca agaccggcct gaccgatctg gcggatctgg ctgtgcagcc
     4381 aaagcggggc accgacggca cgctcctcaa cggcgtgatg cgcgccctgg tcgaggcgaa
     4441 ggggggagcg cctgccgcgc tcacggcgat gctcatgcag tacacccccg aggcggttgc
     4501 tgccgaaacg ggcgtcagca ccgacgagct ccagcaactg gccgaggcct acacggcggc
     4561 gcggcatccc gtgctcatct atggacgcgg gatcacgaag cagaatgacc cgcgcttgat
     4621 tgcgactatg caggcgctga cgctcgtcgc cgggcatgcc acggccacgt ccttcccgat
     4681 cctcgggctg cgccggtcgg ccagcagcac gggcgcgttc gatctgggag tggacccgac
     4741 ccgcctgcct ggccggctcc gctttacgcc ggccgaggcg aatcgctttg cttcggcgtg
     4801 ggggaagccg gtgccggagc ccacgagctt caacgccaat gagttcgacc cgaagacgat
     4861 tggtgcgctt tacctcctca tcggcgatga taccctgccg caagaagagg agatggcgga
     4921 gcggctggcg gagctggagt tcgtggtagt gcaggccacc tatcgaacgc cgctcaccga
     4981 ggtggcggac gtggtgctgc ccgcgccaac ctgggcggag caatcgggca ccttcaccgg
     5041 gatcgatggc caggtgcgcc cgctgcgcca ggcggtgacg ccgcccgaag gcgtgcgcat
     5101 gccgtgggat gtgctctgct ctctggggaa cgcgctgggc cttaagggct cttatgccaa
     5161 cgtggctgaa gtggcgcacg agattggccg gctggtgccg gagtatgccg aggccctggc
     5221 caccggggag cgagcggtcc acttccgcga cgcatccttc gatgtgcgcc ggtcagagct
     5281 gcgcgcgcca gactaccttg ctgagtcggg aggtcgttaa gcatgagtga caagcccaag
     5341 attgcgacgg tctggctggg cggatgctct gggtgccaca tgtcgctgct ggatctggac
     5401 gagggtctgc tcgatctggc caaggcggcg acgatcaccg ccagtccgat caccgatatc
     5461 aaggagccgc ccgaggtgga tgtctgcctg gttgaggggg cggtgtgtaa caccgacaac
     5521 ctggaagtgc tccgcaagtg tcgcgagcgc gcgaagatcc tggtggcgtt cggcgattgc
     5581 gcctgcttcg gcaacatccc ggcgatgcgc aacttcatct cgctggagga gacgctccag
     5641 cgcggctacg tggatacgga gagcacggcg tccggcgagg tgccccgcgg cgaagagctg
     5701 cccgcgctgc tggagaaggt tcagccgatc agctactatg tgaaggtgga tgccagcctg
     5761 cccggctgcc cgccctcggc agaggcgatc ctcttcgcgg tgaaggagct gctttcgggc
     5821 cgtctgccga agcccgaagg ggaactgctg cgctacgatt gagatgtgag atgtgggatg
     5881 cgggagggat gctgccctga gcgagttgtg aggggatagc cagggggcca ccgcgcgcgg
     5941 agaggatccc cggtggtgcg tccagccatc tcgcgtcacg cgtctcccct cctgcatccc
     6001 agatcacgga gagggatatg tctaggactg ttacgataga gccggtaacc cgcattgagg
     6061 ggcatgccaa agtgacggtg cgcctcggcg atgacggcaa ggtgcagcag gcgcgcatgc
     6121 acgtggtgga gttccgcggc ttcgagaagt tctgcgaggg gcgccacttc actgagatgc
     6181 cgacgatcac cgagcgcatc tgcggcatct gccccgtcag ccaccacctc gcctccgcca
     6241 aggcaggcga tgcgattctc ggtgtgacga tcccgcggac cgcctacatg ctgcgcgagc
     6301 tgatgcacat ggggcagatc ctgcagtcgc acgccctcag cttcttccac ctgtcggcgc
     6361 cggacctgct gctcggcttt gatgccgacc cagcggtgcg caacgtggtc ggcctcatcc
     6421 agcagcagcc ggagattgcc ctcaagggga tcaagctccg caagttcggg caggagatca
     6481 tcgcccgcgt cgccgggcgc aagatccacc cgaccttctg tgtgccgggt ggcgtgaacc
     6541 agcgcctggc cgcgtccgac cgcgaggcga tgctcgccgg gatcgacgag gcgattgccg
     6601 gctgtcagga tgcgatcagc ctgatcaagc gctactatgc cgatcacgcc gacgaggtgg
     6661 cacgcttcgg gaacttcccg tcgctgtatg ccggcctcgt caccgaggat ggcagcctgg
     6721 agcattatga cggccggatg cgcttcgtcg atgctgatgg caaggtgctg gagaacggga
     6781 tcgacccgag caattacctg gattacatcg ccgagacgac ggaggagtgg tcctacctga
     6841 agttcccgta cttcaagcgg gtgggctatc ccgagggcgc ctaccgcgtc gggccgctgg
     6901 gccgcctgaa cgtggcagac agcgcgcgca cgccgctggc gaacgaggag ctgaacagct
     6961 tcaaggcgct cggcgatggc cggccggtgc agggaacgct gtactaccac tacgcacgca
     7021 tgatcgaggc gctgtatgct gtggagcgtg cccgccagct gctggaggac ccggagatca
     7081 tgggccgcga catcgtggcg acatccaaca gcctgcagcc gcgaggcgtg ggcgtcatcg
     7141 aggctccgcg cggcacgctc ttccacgact accacgtgga cgagacggga cgcctggaga
     7201 aggtgaacct gatcgtctcc accgggcaga acaacgtggc gatgaacgaa gccgtccacc
     7261 cactcgcggg gatgctcaac cgcgtggaag ccgctatccg cgcctatgat ccatgcctct
     7321 cctgttccac gcacgccatc ggccagatgc cgatcgtcgt ggagctcatt ggcccggatg
     7381 gcgagcagct cgaccgcgcg acacggtagc cagcgcgatc tgctgcgaaa cgcgtcggag
     7441 agaccctcga atgggccggc agcgggagct cccgctgccg gcccctcttg ctttgcgccg
     7501 cggcccccgg ggggaggggc tttctctccc tggatgagag ccgggccagc ggcgggcctg
     7561 gattggggtt gcgagacgat ggtgccacgg gtgttgattg ttggttgcgg gaatgccagc
     7621 cggcgtgatg acggcgtggg cctttatgtg atccgagggc ttgcccgcgc gtttggcctg
     7681 cccctcgacg agggtgcgga cgaggtgagc gtcgcgtgcg aggtggagac gccggatggc
     7741 ccggtccgcc tggagttgcg cttcgagcag cagctcgaca tcgtgttggg cgaggagctg
     7801 aaagacgtag acctcttcgc cgtcatcgac gcgcacaccg gcgtctatcc ggaagcgctg
     7861 cgccgcgtgg aggcggagcc gggctacacc tcctcgctga cgtcgcacca cctcacggcg
     7921 gatactctgg ccggcctttc ccaggcgctg cacgggcgcg cgccccgtac cttcatcttc
     7981 tccgttttgg ggcacgattt caactttggc gaagacctca cgccgcagac gcaggctgcc
     8041 gccgatgaag cggtccggca gctccgcgac ctggtgcaag aggccgcgcg cgccgcgcgc
     8101 gcttgattca tagggcccga ggctcttctc agctagagtt tgcgccggcc cggggcgggg
     8161 tagagtatcc cggagagggg gccggggatg gccttgctgc tcgtcacgtt cgcggtaggg
     8221 ctgctcgcgg gaggaatcgc ttcggtggcg ggcttcggta tcggcagcct gctgacgccg
     8281 ctcctcagtg tgccgctcgg cacgcgtctg gccgttgccg ctgtttccat cccgcacgcc
     8341 gccggcacgg cggtgcgcct ctggcgcctg cacgatcagg tgaaccgccg ggtgctctgg
     8401 agcttcgggc ttgcgagcgc ggcgggcggt ctggccggtg ccatcgggaa cgcggtgatc
     8461 gccaccggct tcctcaccct cgtgcttgcc gggctactgg ttctcgccgg ttcgatggag
     8521 ctcaccggcc tcagcagccg catgcgcttc gcggggacgg ctgcctggat cgcaggcggg
     8581 ctctctggcg ctttcggcgg cctggtcggg aatcagggcg ggattcgcac cgcggctctc
     8641 ctcgggttcg acctccagcg cgacgccttc gtcgccacgg ccacggcaat cgccttgatg
     8701 gtggatctcg tccgcgtgcc ggtctacctg gccacgcaag gcaccgccat cgcggcacag
     8761 tggccggtgg taaccgcggc caccgccggc gtcctcatcg ggactcactt tggcacgcgc
     8821 gtcctccagc gcattcctga gcgcctcttc cgcacgctcg tctccctcct catcctggca
     8881 ttgggaatcg ccctgggcgt gcgggggatt cggatgctat gatacccggc gagtgagcct
     8941 cagagctttg gcagcttctc gcacgcttcg gcgagcaggt ccaccgtgtg catggcgcgc
     9001 acctggccag ccaggcccgc gcggtggatc ccgaggcgaa gctggagcag gcacccgggg
     9061 ttgcccgtgg tgacgaccgt ggcgccggta cgccggagct gctccatctt ctcgtcgagg
     9121 atccgcatcg agacgtcgaa gtggagcagc gagtacgagc ccgcggagcc gcagcagcgg
     9181 tcgctctccg tcatctccac cagctccagc ccgggcaccc ggcgcaagag gtcgcgcggc
     9241 tctgttttca ccttctggac gttcgccagg tggcaggagt cctggtaggt gacgcgttca
     9301 ttcacgcgcc gcagggcggg cagcgcgccc tgggtggcga tcagctccgc gacgtcgcgc
     9361 acccgggcgg caaagcgccc ggcgcgttcg gcccactgcg ggtcgtctcg caggaggtgc
     9421 gggtattcga ggagctgcgc tccgcaaccg cccgcgttgc tcacgtagaa ctccgcgccg
     9481 gaggcctcga aggcggcgat gttctgcttc gccagcctgc gcgccatttc cagttcgccg
     9541 gcgtgggcat gcagcgcgcc gcagcacgtc tgccccttgg ggatgaccac ctcgaacccg
     9601 cacgcgcgga gaagctgcac cgtgcgccgg ttggtccggt ggagcagcgc ctcggcaacg
     9661 caccccgtga ggaacgccac tcgtgcgcgc cgctcgccgg cagccgggta gagggcgcca
     9721 tgaggcaggc gctcggtggg aggggcaaga tcggtg
//
LOCUS       DAOSCV010000030         9445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4767_length_9445_cov_3.119169, whole genome shotgun sequence.
ACCESSION   DAOSCV010000030 DAOSCV010000000
VERSION     DAOSCV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4767_length_9445_cov_3.119169"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLY56_01475"
     CDS             <1..352
                     /locus_tag="PLY56_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20180.1"
                     /translation="TYDTLIQTLDPDLLDPECVDAARKVQDAMKGVRTGPFPHETRAE
                     IYRHFIREVRRWNKDVLLYVSTETREMWDELKDELGQDPRRYICACGSAAVPGKKRAL
                     SRAFRYSTYHPTPL"
     gene            741..1889
                     /locus_tag="PLY56_01480"
     CDS             741..1889
                     /locus_tag="PLY56_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HOJ20181.1"
                     /translation="MYDLIIVGGGPAGSSAGRTAGRLGLRALLLEKEVFPREKPCGGA
                     LSARALASLDFLLPPALQAQALTGARVCYRGRKVEAHLSAPYAFLVSRSDFDHFLLTE
                     AEKAGIEVHQGEKVLGLCETDGSISVRTPVGVYRGRCALIAEGAQGHLKGRARRRETR
                     AEQGFAIVTEIPVTQPQAGIAEIHFGMSRWGYGWIFPHAGYLSVGVGGLLPAMPRIRE
                     RLQVFLRKNGLPETSRCRGHVIPMGGIRRNLVNGRILLCGDAAGFADPLSGEGIAYAI
                     RSGQLAAEAVHRALSERIEALEEYEAACERAFGGELRDALFLARLVHRFPAAFHRLFS
                     SHPRVVAAYLDVVAGIRSYRSFLAWALPRMPAYLALREICRPTPAKKI"
     gene            2165..3610
                     /locus_tag="PLY56_01485"
     CDS             2165..3610
                     /locus_tag="PLY56_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ20182.1"
                     /translation="MKRKPNLLILGIDSLRADHMSCYGYPRLTTPHMDRFATEGVLFE
                     NTFSPHIPTTPGYACMLTGMDCFNTQIVALRHKGGLRPEVRTLPEILKENGYNTATVQ
                     YPFRGFDKVLEYSGWGGDPGGKMPKAENLNKVAIPELDRMADAPEPFFLFLRHLDPHT
                     PYLPPAPYERIFYHGNEYDPENKSMEPVMAFKPFCDYFASWLPGPVTDKDYVIAQYDG
                     AVAYMDAAIQSIFTALEAKGILDDTVVVITADHGETLYEHECWFDHHGTYDNVLHVPL
                     IIRYPEKVPAGLRLSGYNQNWDIVPTILELLDIQVSDYQFDGRSLMDMVRGETPHHDT
                     EFYFTECTWMRKHGWRTPEWKLILALEPDFHFKPPVELYNLLDDPEENNNLAEKEPEV
                     VEFLKARMEAHIAQREKATGLPNPIHHQGDWHGHEGIGAFKSSQQAYDTLHIGNPGEA
                     AKLQARLGGRRRLPGSALPRWRWRGRTSGPA"
     gene            complement(3579..4457)
                     /locus_tag="PLY56_01490"
                     /pseudo
     CDS             complement(3579..4457)
                     /locus_tag="PLY56_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            complement(4721..6988)
                     /locus_tag="PLY56_01495"
     CDS             complement(4721..6988)
                     /locus_tag="PLY56_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20183.1"
                     /translation="MKIFDSMPFAKELLAPTTSESFRRPGAQARPGEVILDGSWSVAV
                     ESDAPLARTAGEHLTRFLADSLGVALGTSGKPIRLRIDSSLGENPETHLVRVGATEIE
                     AVGAGPEGVLRAVFRLEARMRERGGPFLATGEERRAPLFRNRIHRSCFSTFYIEELTG
                     YDGPPLDARWFSPGMAYPGYAEEDAGPEMFYHDNLLMRLAEQGFNGIWVRAALSLVAK
                     VWPFPEFGADSDRILERLNRLCERAARCGLKVFLYWQEPMGMRDDHPFWKAHPQVRGS
                     NSPYKPMVHLCTSTPEVQEYLREGTRYVFEKVPGLSGVLQISASEFPSHCYSHLRRPE
                     DPKELEKQVAEGKLCPRCAPYLPQEIIAQILTLVRDGAKAANPNAEVIAWNWSWSHYE
                     PDPQRGVLERLPADVIVMGDYERGEPAEACGFQYTNDEYSIKVVGPSQRFRGVAQFQK
                     EHGRPVYAKLQLGTTHENPSIPYLPVPQKIAQKYYTLKETGVSGMMTCWNFGNMPSLA
                     TEVAGEFSWDPQPATIEDGLRRIAARHFGEEVADEVVAGWAIISRAHDDFPSSIPVMY
                     QGPISRGPAFPLVFDRVNRKFPNSWLLDKEIEGDLLNWVAPFGAEKVVECFRSEVAKA
                     APGVAILEKALEKTTGADRERLARDLGVIRFHLIQLESSANVVEFLLARNAMYDATDP
                     EEKRRHLDRMEEICRAEWENASAALPLVDADPRLGWHGEAYGYMINRPLIEQKLAGLK
                     VILEERIPQERAKLG"
     gene            complement(7180..7929)
                     /locus_tag="PLY56_01500"
     CDS             complement(7180..7929)
                     /locus_tag="PLY56_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20184.1"
                     /translation="MAKSLHGVQVLVGVSRSQLRWLFAAIWAANVGLLASNFLFAAGW
                     APHPSVVNQLNMDEETAFGAWYPSALLILLALSAALHYLMDRHAKHEPPSASAGWLHL
                     AGIALIISADEVCGVHEYADRYYSNEVSTQFLGIGVSWTLLFLPLILLSAMALARLAI
                     HSLKRTPVARTLALAGLCCWLGAVGLEMAQWAFLDGEPLPVIGAYEPALEEGLELVGT
                     TLLLHAVFVSMSSWTAHWRQVLLGAPPSRAS"
     gene            complement(8242..8619)
                     /locus_tag="PLY56_01505"
     CDS             complement(8242..8619)
                     /locus_tag="PLY56_01505"
                     /inference="COORDINATES: protein motif:HMM:NF018767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOJ20185.1"
                     /translation="MSTGNGHRVWIFPDGDLPPAGEPDLPLEGHESLVVLNTGDEDAH
                     ILADVYFPDKEPETGIKLLAPARRVTCFRLDKPIGEQGFRVPFGQYALRLTSNVPIVA
                     QIGRADVRQPNLAYYTTMGFPGD"
     gene            complement(8668..>9445)
                     /locus_tag="PLY56_01510"
     CDS             complement(8668..>9445)
                     /locus_tag="PLY56_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ20186.1"
                     /translation="LGKSPWLQSVHFTGDAAMQHPEIVKQVAAAGHEVGAHSLYHETV
                     GDPIFEIPGVKPLLPEEVPLRVRRATEAVARALGEQPVSWRCPRLWGSTAVVNALEEL
                     GYIADASYPLYYYREQLVPYHPSREDWTKKGDSKVLQIPNFADMTIESKDPYGRDRDQ
                     WPLWRTESAASLMTHVDNYVGYVRERGLPAVLCFYMHPWEFWPMASEYHFGEGTVVPD
                     PFIVKNCGDYALEQLGVLIDLLKERGAEFTTAKGLAATWK"
BASE COUNT         1645 a         3148 c         3123 g         1529 t
ORIGIN      
        1 cacctacgac acgcttatcc agacgctcga tccggatcta ctcgaccccg agtgcgtgga
       61 cgccgcgcgc aaggtccaag acgcgatgaa gggcgtgcgc accggaccct tcccgcacga
      121 gacgcgcgcc gagatctacc gccacttcat ccgcgaagtg cgccgctgga acaaggacgt
      181 gctcctctac gtaagcaccg agacgcgcga gatgtgggac gagctgaagg acgagctagg
      241 ccaggatccg cggcggtata tctgcgcgtg cggctccgcc gcggtgccag ggaagaagcg
      301 ggcgctctcg cgtgccttcc gctactccac ctaccaccca acgcccctgt aaggataggg
      361 ccggcgcggg gggcatcccg ccggggcacc tcgcgccggc agccgcgcat ctgccgcgcg
      421 cgacctaacc gtctgcccgg ctccatctcg gtgtttggga acctgcccca cggggaatga
      481 ggccctgtgg ctccaccgct ctttgctcgc gggggcgtat ctccggaggg ccgggcgtgc
      541 ccgcgtcgcg ggttcacccc tttgctccca atggatccag ccggctgcct gtggctgcgt
      601 agagctcctg cgcaaggggc ggagaacctt ccccgcaagg gcacagagga gcgccatcac
      661 ctacacttca tccaggcgct ctgaggccgc cgtcgtgagg cccacagacg ggctttgctc
      721 accactgggg gggcagcggc atgtacgacc tgatcatcgt gggagggggg cccgcgggct
      781 cctcggcggg ccggaccgcc ggacgcctcg gcctgcgcgc actgctgctg gagaaggagg
      841 tcttcccgcg ggagaagccc tgcggcggag ccctctcggc ccgggccctc gcttccttgg
      901 atttcctgct cccgcccgca ctgcaagccc aggcgttgac tggcgcgcgt gtctgctacc
      961 gcggtcgcaa ggtggaggcg cacctttcgg cgccctatgc cttcctggtc tcgcggagtg
     1021 acttcgacca cttcctcttg accgaggcgg agaaggcagg catcgaggtc caccaggggg
     1081 agaaggtgtt gggcctctgc gagacggacg gcagtatctc cgtgcgcact ccggtgggcg
     1141 tctatagggg ccgttgcgcg ctcatcgccg agggcgccca gggacatctg aaggggcggg
     1201 cgcgccggcg cgaaacgcga gcggagcagg gattcgccat cgtcacggag attccggtga
     1261 cgcagccgca agccggcatc gcggagatcc actttgggat gtcgcgctgg ggctacgggt
     1321 ggatcttccc ccacgccggt tacctctcgg tgggcgtcgg cgggctgctg ccggccatgc
     1381 cgcggattcg ggagaggctg caggtgtttc tgcgcaagaa cggcttgccc gaaacgtctc
     1441 ggtgccgggg acacgtgatc ccgatgggtg ggatccgtcg aaatctcgtc aacggccgca
     1501 tcctcctctg tggcgacgcc gccgggttcg ccgaccccct cagcggcgag ggaattgcct
     1561 acgcaatccg gtccggtcag ctggccgccg aggcggtgca tcgtgccctt tccgagcgga
     1621 tcgaggcact cgaggagtac gaagccgcgt gcgagcgcgc gttcggcggt gagttgcggg
     1681 acgcgctctt cctggcccgc ctcgttcacc gtttcccggc agcattccac cgcctcttta
     1741 gcagccaccc ccgggtggtg gctgcctacc tggatgtggt ggccggcatc cgaagctatc
     1801 ggagcttcct cgcgtgggcg ctgccgcgga tgcccgcata tctcgcgctc cgtgagatct
     1861 gccgcccaac cccggccaag aagatctgaa cctacccgag agcgcgagcc acaggcgggc
     1921 ccgcgcgcgc atccccccgg ctcccgtcag gagaagtgat tgccacatca ccagcaggtg
     1981 ttcttccgcc cgggtgcgaa tctcatggcg atacggttgg gtttctcgga tcccgcgacc
     2041 ggcttcggcc ccgaagggcc gaacaacgag gccgagcgcc cgcgaagcgc gtcgggacgc
     2101 tcctctcttc gcgttcgccc gtgccgcgaa ggccggtacc gcgcacggag gaaagaggat
     2161 tgccatgaaa cgaaagccga acctgctgat cctgggaatt gactcgctgc gcgcggacca
     2221 catgagctgc tacggctatc ccaggctgac gactccccat atggaccgct ttgccacgga
     2281 gggcgtgctg ttcgagaaca ccttcagccc gcatatcccc accacgcccg gctacgcgtg
     2341 catgctcacg ggcatggatt gcttcaacac gcagatcgtg gccctgcgcc acaagggcgg
     2401 cctgcgcccc gaagtgcgca cgctccccga gatcctgaag gagaacggct acaacaccgc
     2461 cacggtgcaa taccccttcc ggggctttga taaggtgctg gaatactccg ggtggggtgg
     2521 cgaccccggc ggcaagatgc cgaaggcgga gaacctgaac aaggtcgcca ttccggaact
     2581 ggaccgcatg gcggacgccc ccgagccgtt cttccttttc ctgcgccacc tcgacccgca
     2641 cacgccgtac ctcccgccgg caccctacga gcgcatcttc tatcacggga acgagtatga
     2701 cccggagaac aagtcgatgg agccggtgat ggcgttcaag ccattctgcg attacttcgc
     2761 gagctggctg cccggtccgg tcaccgacaa ggattacgtc atcgcccagt acgacggcgc
     2821 cgtggcctac atggacgcgg cgatccagtc gatcttcact gcgctggaag ccaaagggat
     2881 cctggacgac accgtggtcg tgatcaccgc cgaccacggc gagacgctct acgagcacga
     2941 gtgctggttc gaccaccacg gcacctacga caacgtcctc catgtgccgc tgatcatccg
     3001 ctacccggag aaggtgcctg ctggcctccg cctgtccggc tacaaccaga actgggatat
     3061 cgtcccgacc atcctggagc tcctcgacat ccaggtgagc gattaccagt ttgacggccg
     3121 gagcctgatg gatatggtcc gcggcgagac gccccaccac gacaccgagt tctacttcac
     3181 ggaatgcacc tggatgcgca agcatggctg gcgcacgccg gagtggaagc tgatcctggc
     3241 cctggagccg gacttccact tcaagccacc ggtggagctc tacaacctcc tggacgaccc
     3301 cgaggagaac aacaacctgg cggagaagga gccggaagtc gtggagttcc tcaaggcgcg
     3361 catggaggcg catatcgccc aacgcgagaa ggcaacgggc ctccccaacc cgatccatca
     3421 ccagggcgac tggcacgggc atgagggcat cggcgcgttc aagagctcgc agcaggccta
     3481 cgacacgctg cacatcggca atccgggtga ggcggccaag ctgcaggctc gcctcggtgg
     3541 aaggcgccgg ctcccaggtt ctgccctgcc acggtggaga tggcgaggcc gtacgtcagg
     3601 gccggcatga aggcgatctg atcgatgcgc gagcccgcgc cgtaggccgc gacggcagcc
     3661 ggcccgaacc ggttgacgat gctgatcaca atgacggccc ccagcgatac gagcacctgt
     3721 tgtgccgccg aaggcagccc gacccgcacc agggtcaaga cggtatccga gtccagattg
     3781 gggcagcgcc agtttggggt gacgagcgtc ggcttccatt ccaggtaaaa cagcagcgcc
     3841 gccagcgtga tcccctgggc aatcacgttg gaccacgccg cgccattgag gccaagccgc
     3901 gggaagccga gcaggccgaa gatcagcagg ggatcgagcg ccgtgttcag caggacggag
     3961 gccgtctgaa aatagagcgg ccgtctggag tcgcccaccc cctgcatcat cgagcgcgtc
     4021 aggaagaggc catagctgaa cggcagcgcc agcaggtaaa ctcggatgta gccaaccgcc
     4081 agcggcagca cctcgggcgg cgtcctcatg gcgcgcaggg ctcgaggagc gaggagctcc
     4141 ccgatcagaa gcaggatgag gctgaggagg ccaatcagcg ccagggagct atcgaccacc
     4201 ttgcgcagtt cgccgcggcg atgcgcgccg aagtactggg cgatcaggac gttggtggcc
     4261 agcgtcagtc cggcgccaag ggcgatgaga gcaaagacta tcgggaagct gaccgtgatg
     4321 gcggcgagcg ccaccgtgcc caagaaccgc cctacccaga tcgcgtcgat gaagctgtag
     4381 gccgtttgca gcaggctgcc cgcgagaatc ggaagcgcga agaggaggag gtgcctcgga
     4441 gtgctcccgg tcgtcatgtc gcgcccatac tggcgcgcat ccgtgcttgc ccggaatcca
     4501 ggcggttgtt ccctcacaag ggtcccctct cgttctgtgg gcagaggccg aatggcaacg
     4561 ccatcccggc catcacgccg cgcggaacca gagggagttt acccaccgcg cgccgaagcg
     4621 caacacgctc ctcgatttca cccggcgccc gtgcgcgccg ggcagaccat gcgattgccg
     4681 gggagcgccc tcgcgcgcgg ggcgctcccc agctgcttac ttacccgagc ttggcccgct
     4741 cctgggggat gcgttcttcc aggatcacct tcaggccggc gagcttctgc tcgatgagcg
     4801 gacggttgat catgtagccg tacgcctcgc cgtgccagcc cagccgcgga tcggcatcga
     4861 ccagcgggag cgcggcgctg gcgttctccc attccgcccg gcagatctct tccatccggt
     4921 cgaggtggcg gcgcttctct tccggatcgg tcgcgtcgta catagcgttg cgcgcgagga
     4981 ggaactccac cacgttcgcg gagctctcca gctggatcag gtggaaccgg atcacgccga
     5041 ggtcacgggc gagacgctcg cggtcggcgc cggtcgtctt ctccagggcc ttttccagga
     5101 tggcgacgcc cggagctgcc ttagcgacct ccgagcggaa gcactccacc accttctccg
     5161 cgccgaaggg ggcaacccag ttgaggagat caccctcgat ctccttatcg agcagccacg
     5221 aattggggaa cttacggttg acgcggtcga agacaagggg gaaggcaggg ccacggctga
     5281 tcggcccctg atacatcacc ggaatgctgc tcgggaagtc gtcgtgcgcg cgcgaaatga
     5341 tcgcccatcc ggcgacgacc tcgtcggcca cctcctcacc gaagtggcgc gcggcgatgc
     5401 ggcgcagccc atcctcgatg gtggccggct ggggatccca cgagaactcg ccagccacct
     5461 cggtggccag ggagggcatg ttcccgaagt tccagcaggt catcatcccg gagacacccg
     5521 tctccttcag ggtgtagtac ttctgcgcga tcttctgggg caccggcagg taagggatgc
     5581 tcgggttttc gtgcgtggtg cccagttgca gcttcgcata gacgggccgg ccgtgctctt
     5641 tctggaattg ggccaccccg cggaagcgct gtgaaggccc aaccaccttg atgctgtact
     5701 cgtcgttggt gtattggaag ccacacgcct ctgccggctc gccgcgctcg tagtcgccca
     5761 tcacgatcac atcggccggc aggcgctcca acacgccgcg ctgcgggtcc ggctcgtagt
     5821 ggctccagct ccagttccag gcgatcacct cggcgttcgg gttggccgct ttcgcaccgt
     5881 cgcgcaccag ggtgaggatc tgcgcgatga tctcctgcgg caggtagggc gcgcaacgcg
     5941 ggcagagctt cccttcggca acctgcttct ccagctcctt cgggtcttcg gggcggcgca
     6001 ggtgcgagta gcagtggctc gggaactccg acgcggagat ctgcaggacg cccgacaagc
     6061 ccgggacctt ctcaaagacg taacgcgttc cttcgcgcag atactcctgc acctcgggcg
     6121 tgctggtgca gaggtggacc atcggcttgt agggcgagtt ggagccgcgc acctgcgggt
     6181 gcgccttcca gaagggatgg tcatcgcgca tccccatcgg ctcttgccag tagaggaaga
     6241 ccttgaggcc acagcgcgcg gcacgctcgc agaggcggtt caggcgctcc aggatgcggt
     6301 cggagtccgc gccaaactct gggaacggcc acaccttggc gacgaggctg agcgcggcgc
     6361 gcacccagat gccattgaag ccttgctctg ccaggcgcat caggaggttg tcgtggtaga
     6421 acatctccgg gccggcatcc tcctcggcat agccgggata ggccatgccg ggcgagaacc
     6481 agcgcgcgtc cagcggcggg ccatcatatc cggtcagctc ctcgatgtag aaggtagaga
     6541 agcaggagcg gtggatgcga ttccggaaga gaggcgcccg gcgctcctcg cccgtagcga
     6601 ggaatgggcc gccgcgctcg cgcatccgcg cctccagccg gaaaaccgcg cggaggacgc
     6661 cctccggccc ggcgccaacc gcctcgatct ccgtggcgcc cacgcgcacc aggtgcgttt
     6721 ccgggttctc gccgaggctg gaatcgatgc gcagccggat cggcttcccg gacgtgccca
     6781 acgcgacgcc cagcgaatcc gccaggaagc gcgtcaggtg ctcgccggcg gtccgggcca
     6841 gcggggcgtc gctctccacg gcaaccgacc agctgccatc cagtatcacc tctcccgggc
     6901 gcgcctgcgc ccccggacgc cggaacgact cgctggtcgt cggcgccagc agctccttcg
     6961 caaaaggcat gctatcgaag atcttcaagg ctcatttcct cctggaacgc gccggccagc
     7021 gaagggaccg gcgacgcggc gggccacccg cagtttcttt cccctcggcc aggccagccg
     7081 gcttgcctgg cgctaatagg atgtaccgga ctccttcgct atgcaccgcc cgctcccttc
     7141 cgaggcccgg taccccggag ctctttgggg atggcgctgc tacgaagccc gagatggagg
     7201 cgcgccaaga agcacctggc gccagtgcgc ggtccaggaa gacatggata cgaacacagc
     7261 atgcaggagc agtgtcgttc ccacgagctc cagcccctct tcgagagcgg gctcgtaggc
     7321 accgatcact ggaaggggct cgccatccag gaaggcccac tgtgccatct cgagccccac
     7381 cgctccgagc cagcaacaga ggcccgccag tgccagcgtg cgcgcaaccg gagtccgctt
     7441 cagggaatgg atggccaggc gcgccagggc catcgcgctg aggaggatga gtggcagaaa
     7501 caggagggtc cagctcaccc ctatccccag aaactgcgtg ctcacctcgt tgctgtaata
     7561 ccggtcggca tattcgtgga cgccacagac ctcatcggcg gagataataa gggcaatgcc
     7621 agcgagatgg agccacccgg cggaagcaga gggtggctcg tgcttcgcat ggcggtccat
     7681 caggtagtgc aaggcggcgc tcagcgccag tagaatcagg agggcgctcg gataccaggc
     7741 tccgaacgct gtctcttcgt ccatgttgag ctggttgacc acggaagggt gcggtgccca
     7801 tccagccgcg aacaggaagt tgctggcgag aagaccgaca ttcgccgccc agatcgccgc
     7861 gaagagccag cgtaactgcg aacgcgagac gccaacgagc acctgcactc catgaagtga
     7921 cttcgccaca ctcatctccc tacgtccacg ggagcggtgg cttgcatcgg agatgccgtt
     7981 ctgatagaga gttgacggtt gagggaactc cgcaggcctc tattctggcc ggcggccttg
     8041 atgtgccggt ggggccaaac cgtgccagcc ggagcgatgg gcgaggtatg cagggggagg
     8101 tgatgcccgg caagaagact tgcctctccc gggcataccc caggggcggg aagggctggg
     8161 ttggagctcg cgcggcgccg gtgccgctct tctccagcgc gccacccgcc cggggggtag
     8221 aaacgaccgg ctggccgggc tttaatcgcc ggggaagccc atggtcgtgt agtaggccag
     8281 gttcggctgg cgcacgtcgg cgcggccgat ctgggccacg atcggcacgt tgctcgtgag
     8341 gcgcagcgcg tactgtccga acggcacccg gaagccctgc tcgccaatcg gcttgtcgag
     8401 tcggaagcag gtgacgcgtc gggcaggcgc cagcaacttg atgccggtct ccggctcctt
     8461 atcggggaag taaacatccg ccaggatgtg ggcgtcctca tcaccggtgt tgagaacgac
     8521 cagcgactca tgcccctcca gggggaggtc cggctcgcca gccggcggca ggtcgccatc
     8581 cgggaagatc caaacgcggt gtccgttgcc tgtgctcatc ctcttctcct tggattccag
     8641 atccgcgcgg ggaggcctgg ccgcacccta cttccaggtc gcggccaggc ccttcgccgt
     8701 cgtgaactcc gcgccgcgct ccttcagcag gtcgatcagg acgccgagtt gttccagggc
     8761 atagtcgccg cagttcttga cgatgaaggg atcgggcacg acggttccct cgccgaagtg
     8821 gtattcggag gccatcggcc agaactccca ggggtgcatg tagaagcaga ggaccgcggg
     8881 caatcctcgc tcgcggacgt agcccacgta gttgtcgacg tgcgtcatca ggctggcggc
     8941 actctccgtg cgccagaggg gccactggtc gcggtcacgg ccgtaaggat ccttgctctc
     9001 gatggtcatg tccgcgaagt tggggatctg aagcaccttc gagtcgccct ttttcgtcca
     9061 atcctcgcgg cttgggtggt agggcaccag ctgctcgcgg tagtagtaga gcgggtagga
     9121 tgcatcggcg atgtagccca gctcctccag ggcattgacg acggcggtgc ttccccacag
     9181 gcgagggcag cgccaggaga caggctgctc gcccagcgca cgggcgaccg cttcggtggc
     9241 gcggcgcact cgcaggggca cttcctccgg gagcagcggc ttcactccag ggatctcgaa
     9301 gatgggatcg ccgacggtct catggtagag cgaatgcgcg cccacttcgt gacccgccgc
     9361 ggccacctgt ttcacgatct ccgggtgctg catcgcggca tcgccggtga agtggaccga
     9421 ttgcagccag ggcgacttgc ccagc
//
LOCUS       DAOSCV010000031         9428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4776_length_9428_cov_3.682599, whole genome shotgun sequence.
ACCESSION   DAOSCV010000031 DAOSCV010000000
VERSION     DAOSCV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_9428_cov_3.682599"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..783
                     /locus_tag="PLY56_01515"
     CDS             91..783
                     /locus_tag="PLY56_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20187.1"
                     /translation="MKGNGFTLIELLVVIAIIAILAAMLLPVLERARENARQGVCLSN
                     LKQIGTGVMMYVQDFDEYMPFSWQISNDQNGRIWPDAILPYIKNTGVFNCPSFSTKVG
                     LDSVGKFLRTQMPYAANTCYYGGRAGSASGPVARHPMSKPMAEIRVPADTVFATDFSG
                     VFEAAGEFDVATGYTQPATSTTYQSIFRHSDMCNVVFCDGHVKTLSRGALLQTHAVSG
                     VDVKYLFTIEED"
     gene            complement(1215..1880)
                     /locus_tag="PLY56_01520"
                     /pseudo
     CDS             complement(1215..1880)
                     /locus_tag="PLY56_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
     gene            2345..3037
                     /gene="tmk"
                     /locus_tag="PLY56_01525"
     CDS             2345..3037
                     /gene="tmk"
                     /locus_tag="PLY56_01525"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ20188.1"
                     /translation="MLRPNDYEGKLIVVEGIDGSGKSTQIDLLNKWLQSQGRAVYFSE
                     WNSSDLVKSTTRLAKKRRAFTPTTFSLLQATDFADRWENHIQPLLKAGAIVLADRYAF
                     TAFARDVARGVDRQWVRNLYSFAPRPDIALYFRVPLHIAVDRILSSRARIKYYEAGMD
                     LGLSDNVVTSFRLFQQRILDEYDKMVDEFGLTVIDGTLPIHEQQLLVRSITHRVLLGG
                     QEQLAPGNVGGE"
     gene            3040..3726
                     /locus_tag="PLY56_01530"
     CDS             3040..3726
                     /locus_tag="PLY56_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOJ20189.1"
                     /translation="MREATFYGAGFPYAPVPQLEGRLIVLEGTDGVGRSTQIQLLKQW
                     LEEEGYGVSNTGLARSPLTQPGLDAAKAGHTMGRFTMSLFYATDFADRLENQILPALR
                     AGFYVLSDRYFYSIIARDVVRGADPEWARKVYGFALKPDIVFYLKVDVPTLVQRMLQG
                     RGFNYWESGMDIRCAHNLYDSFCRYQALVIEQFDQMADEFGFITIDATRSAREVFEDL
                     RAHIRKLETP"
     gene            3847..4851
                     /locus_tag="PLY56_01535"
     CDS             3847..4851
                     /locus_tag="PLY56_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOJ20190.1"
                     /translation="MSQERFYPDLTIALLAPRAMAKRVFAPRHYDRLESMATLVDACF
                     ERAADERVDQLAKADIIVSTWGMPRVDEAFLERTPRLRALFYAAGSVKGFVTDALFER
                     GITLSSAAPANAIPVAEYTVAAIILSNKRFWALMRHRRGEAGLPEEVPGNYRHTVGVI
                     AASMVGREVIRLLRGYDLEVLLYDPWVGEEEARRLGVTKVELPELMERSDVVSLHAPN
                     LPSLRHMINGPLLSRMKDGATFINTARGALVDEEALLAELKSGRIWAVLDVTDPEPPV
                     EGSPLYTLPNVIYTPHIAGSMHQECHRMADFALDELDRYLRGEPLRNAIQREALARLA
                     "
     gene            4848..5747
                     /locus_tag="PLY56_01540"
     CDS             4848..5747
                     /locus_tag="PLY56_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20191.1"
                     /translation="MRTDATRRVLRALLTLLTFGTGLAPIGPAHAWSTDGHMVLTEAA
                     VLATPASLPSFFRSDGKAIARDSRDPDFSKHRSLPALRNGEGPEHYLDLELLKGRSLP
                     ATRYGFIHQCARAKLDPDRVGLLPYALAEATERLALAFAESRQRPEDTHVQAKCRYLA
                     GQVAHYAQDLCQPLHTTVHHDGKVRPDGSSPRTGIHSRIDDLITALRVEPSALAARQQ
                     VAPVESLMPAILSELKRSNALVTRVYMLEPKLRVKGARGLAPEVSDFAQERARASVRF
                     TASLYATAWKLSGTLQLPKAAAE"
     gene            6143..8002
                     /locus_tag="PLY56_01545"
     CDS             6143..8002
                     /locus_tag="PLY56_01545"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HOJ20192.1"
                     /translation="MIQTDKLFVHGTLATPRHLRVLTGFEPPRRPALLHDFRRVPTPH
                     ALPHILPEPGATTEGYLIEGIDAEVLARLDRYEENGSLYERRPVVVRAGGEWIQTHAY
                     VGSPTAIGVHPCRDFEPEDRVEYHLLAEMESTLARRFGSAESDIRLGRELLGDAIAEL
                     TRKHWLDPRWSPRALGRALQHAPLPSLEWLREDAHARRFASAYLRLIARIVILNQVED
                     RIRSEFRQALRPQASCYPRTESLLAALAFLNARAEALEAALAGAGLRVYQADYDYVDT
                     VRQAILLADALYDRSLAAEIIARMEINRRPGGTPLGAELEFSPLGARAIRSRRGADPQ
                     LDGLYYFHDFDLARRLWKLGGYRDDHTGVTFDAGRTRGFLEIAIGRLQAEGDLSQPAT
                     TDLWILSELIRETVEFIGIPPHSLHLSLQAEEGRPFTRLPGPEFLFCLLLLGGDLGLD
                     TVGILREKRIHGGEIRTRRGELAFSRWNAHRAVEGGQPPVPVIEFAFPRLAAGRHYEP
                     LLFALKGLQRALNPLPLVGPNGPLDRRYYRQMEQALLQWAAAPTPVDEGIITEFIDVV
                     ATGLRMEREAGHGHSPGECADGLIRVEAALHERNEEIRRISSQGLACAPTQGA"
     gene            complement(8077..9012)
                     /locus_tag="PLY56_01550"
     CDS             complement(8077..9012)
                     /locus_tag="PLY56_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HOJ20193.1"
                     /translation="MANEMGGHQPYPLGEGDRFRMLREGTYRREELDLQRMEDLEHWT
                     IAEGEPDIRGWDILTQEGDHVGKVHDLIASPKTQHVYFILAQIGGLWGMGGRHVLVPL
                     DRIHLNRENHQVRIDAPRDRLENAIEWRAGEEIDYAGAYRYWMEEGMPGRAETHEMAG
                     MRGGEVPAGETRVPLREEEMVATRQVHTGEIEVEKHVVSEQRTIEVPVSHTEVEVERR
                     PVTGEARYHTEGEITEGEVRIPVREEEVHIEKRPVVKEEIIVRQHPETEMERRTETIR
                     REVAEVHKEGDIDVETKGVDVETESNIDRSRRRTG"
BASE COUNT         1610 a         3175 c         2890 g         1753 t
ORIGIN      
        1 gccttcccgg cagccaaagg atataaaggc attcccaact atatggactt tgtaaacccg
       61 gagttttccg gtatctgacc agggggaacc atgaaaggga atggttttac tttgattgag
      121 cttctggtgg tgattgccat tattgctatc ctggcggcca tgcttttgcc ggtgctggaa
      181 agagccagag aaaatgcgcg gcagggtgtc tgcctgagca atctgaagca gattggcacg
      241 ggcgtgatga tgtacgtgca ggattttgac gagtacatgc ccttctcctg gcagatcagc
      301 aacgaccaga acgggcggat ctggccagac gcgatcctgc cctacatcaa gaacacgggc
      361 gtcttcaact gcccctcgtt ctccacgaaa gtaggcctcg attcggtggg aaagttcctg
      421 cgcactcaga tgccctacgc ggcgaacacc tgctactatg gcgggcgcgc gggtagcgcc
      481 agcgggccgg tcgccagaca cccgatgtcg aagccgatgg cggagatacg cgtgcccgcg
      541 gataccgtct ttgcgaccga cttttctggc gtcttcgaag ccgctggcga gttcgacgtg
      601 gccaccgggt acacccagcc tgccaccagc acaacctatc agtcgatctt ccggcactcg
      661 gacatgtgca acgtggtgtt ctgcgatggc cacgtgaaga cgctgagccg tggcgccttg
      721 cttcagacgc acgcggtcag tggcgtggac gtcaagtacc tcttcaccat tgaggaagac
      781 tagcggggtc ttctgcgtgg tgcggggccg ttttgcgcgc ctccgtggcg cgcaaaacgg
      841 cccttctctt gcggaagtta cgaggcgctc cagaagaggt ggacgcggtg tccggccggg
      901 tcgggctcct tgaggaagac cgacttcact ccgcccttgc atgtgggctc ttcgagggcg
      961 attcccttct cctggagaac ccggaccgct tcctcgaagt tggccacgcg aatcgccaca
     1021 tgcgcccgcg gctcctttgg ctccttgaac acctcgatcc gcccggggcc cgggccagag
     1081 acgaagatgg aggagttgcc caccttctcg gagaagccaa acgtcttcgc gtaccaggca
     1141 gccacctcgg cgccgccacc cgcctgcgtg ggatagaggc ccacgtgctc acacccgagg
     1201 aagaggggca cgccccgcgc cttcttcacc caccacaggc actgcgccac tttggccgtg
     1261 agggcttccc agtcacccgc ggcgacgatc tccttcgtga tcagcttggt gccgatcccc
     1321 aggcacgcgg tgccggcctt gatccattgg gtgatgctct cctcggtcgc gtccacgccg
     1381 cccgtcggca tgatccgcgt ccaggggcac gggccgagga tcgccttgac gaatgacggc
     1441 ccgccaactt ccttgccggg gaagagctta acaatctcgc accctgcctc ttccgcggcg
     1501 ctgatctcgc tggcgctgcc gcacccgggc gagtaggcga ccttgcgccg gttacaaacc
     1561 ttcgccacct ccgggttgaa aaccggcccc acgacgaagt tagccccgga attgatataa
     1621 agcgccgccg ttcccgggtc gacgacggaa cccactccca ggatcacctc cgggcgctcc
     1681 ttggcaaagc gggccaccag ctcactgaag acgcggtagg caaggtcgcc tcggttggtg
     1741 aactccacca cgcgagcgcc gccatcggcg caggcagaaa tcaccttcgc cgccgtctcg
     1801 acattcccgt cgtagaaaac cggtaccagc ccggtgtcta gcattgtgtt gagtacttcg
     1861 agtcgttgaa aacgtgccat tggtaacccc ctgtcgcctc attgttagcc acgcgcacgc
     1921 ccccctcctg cctctgtcct gcagcaagcg cgggctttcc tggcccctgg ctgttctgcg
     1981 cgcagctagg gccccgcgct gctttccagc aagcgccctt cgggccaccg catccctgcc
     2041 acatcgccaa cccgagttgc ccaggagcgc agagatcacc agtccgacag ggcttggctc
     2101 acaagcagcg cggagattta cctccgcatg gtggctgctc cccctgccag atacggctac
     2161 acaaagcatt cgcgcctatg gtataatctc tgagagggag atggggtcac accgatctgt
     2221 cggcaagagc acgaccaccc ggcctgtgag caccgccagg ggcggtccgg tgccggtggc
     2281 tttcggaccc gcccgaactt gaggttgctc ctgggcctct gcccggctcg cgaaagggga
     2341 gagcgtgctg cgtcccaacg actacgaggg caagctgatc gtcgttgaag gcatcgatgg
     2401 cagcggcaaa tccacgcaga tcgacctgct caacaagtgg ttgcagtctc aggggcgggc
     2461 ggtctacttc agcgagtgga actcatccga tctggtcaag agcaccacgc ggttggcgaa
     2521 gaagcggcgt gcgttcacgc cgacgacgtt cagtctgctt caggccacgg acttcgccga
     2581 ccgctgggaa aaccacatcc agccgctgct gaaggcaggc gccatcgttc tggccgaccg
     2641 atacgcgttc actgccttcg ctcgcgatgt cgcgcgcggg gtcgaccggc aatgggtacg
     2701 caacctttac tcctttgccc cccgaccgga tattgcgctc tacttccggg tgcctctcca
     2761 tatcgccgtg gaccgcatcc tctcatcacg cgcgcggatc aaatactacg aggctgggat
     2821 ggacctgggt ctctccgata acgtggtcac tagcttccgc ctcttccagc aacgcatcct
     2881 ggatgagtac gataagatgg tggacgaatt cgggctcacg gtgattgatg gcacgctccc
     2941 gatccacgag cagcaactgc tggtgcgttc gatcacgcat cgggtgctcc ttggcggcca
     3001 ggagcagctc gcgcccggga atgtgggagg ggagtgacga tgagggaagc caccttctac
     3061 ggagccgggt ttccctatgc cccggtgccc caactcgaag gaaggctcat cgtgttggag
     3121 ggcacggatg gcgtcgggcg ctcgacgcaa atccagctct tgaagcagtg gctggaggaa
     3181 gagggctacg gcgtctccaa caccggtctg gcgcgctcgc ctctcactca accaggcctc
     3241 gacgcggcga aggcaggcca caccatgggc cggttcacga tgagcctctt ctatgccacc
     3301 gacttcgccg accgcctgga gaaccagatt ctccccgcgc tgcgcgccgg gttctacgtg
     3361 ctttccgatc gctacttcta ctctatcatc gcgcgcgatg tggtgcgcgg tgccgatccc
     3421 gagtgggcac gcaaggtcta cggctttgcc ctcaagccgg atatcgtttt ctatttgaag
     3481 gtggatgtcc ccacgctcgt acagcgcatg ctgcagggcc gcggcttcaa ctactgggaa
     3541 tccggcatgg atatccgctg cgcgcataac ctgtacgaca gcttctgccg ctaccaggcc
     3601 ctcgtcatcg aacagttcga tcagatggcg gatgagttcg gcttcatcac gatcgacgcg
     3661 acccggtcgg cgcgcgaggt gttcgaggac cttcgcgcgc acatccgcaa gctggagaca
     3721 ccgtaggcgc gccatggaga tcaccgcccc ctctcttcca acgccgcctg cccgagcctt
     3781 tcgcccaaat ggccggcgcg ggcgcagtct cgacgcatcc ctccaacggt gtgaaggagc
     3841 aggaagttgt cccaggagag gttctatcca gatctgacga tcgcgctgct ggcaccccgc
     3901 gcgatggcaa agcgggtctt tgcgccccgg cattatgacc gcttggagtc aatggctacg
     3961 cttgtggacg cgtgcttcga gcgcgctgcg gatgagcgcg tggaccaact ggcgaaggcc
     4021 gacatcatcg tgagcacctg gggcatgccc cgcgtggacg aggccttcct ggagcggact
     4081 cccaggttgc gcgcgctctt ctacgccgcg ggctcggtga aggggtttgt cactgacgcg
     4141 cttttcgagc gagggatcac cctgagctcg gctgcgccgg cgaacgcaat cccggtagcc
     4201 gagtataccg tggctgccat catcctatcg aacaagcgct tctgggcgct gatgcgccat
     4261 cggcgcggcg aggccggcct tccggaggag gtgcccggaa actaccggca caccgtgggc
     4321 gtgattgcgg ccagcatggt gggccgcgag gtgatccgcc tgcttcgtgg ctatgatctg
     4381 gaggtgctcc tctatgatcc ctgggtggga gaagaggaag cccgccgtct cggggtcacc
     4441 aaggtggagc tgccggagct gatggagcgc tccgatgtgg tctcgctgca cgcccccaat
     4501 ttaccgagct tgcgccacat gatcaatggg ccgctcctgt ctcggatgaa ggatggcgcc
     4561 accttcatca acacggcgcg aggtgccctc gtggatgaag aggccctcct cgcggagctg
     4621 aagtcgggcc ggatctgggc ggttctggat gtgactgacc cggagcctcc ggttgagggc
     4681 agcccactct acacgctccc gaatgtgatt tacacgccgc atatcgcggg atcgatgcac
     4741 caagagtgcc atcgaatggc agatttcgcc ctggacgagc tggatcgcta cctgcgggga
     4801 gagcccctgc gaaacgccat ccagcgggag gcactggcaa gattggcatg agaaccgatg
     4861 cgacacggcg cgtgctccgc gccctgctca cccttttgac ctttgggacc ggcctggccc
     4921 cgatagggcc cgcgcacgcc tggtccaccg acggccatat ggttctgact gaagccgcgg
     4981 tgctggcgac tccggcctcg ctcccatcct tctttcgatc cgatgggaaa gcgatcgccc
     5041 gcgactcgcg cgaccccgat ttctccaagc atcggtcatt gcccgcgctg cgcaacggcg
     5101 agggccctga gcactacctt gatctggagc tcctgaaggg ccgctcgctg ccggcgacgc
     5161 gctatggctt catccatcaa tgcgcccgtg ccaaactcga tcccgaccgg gtgggtctcc
     5221 tgccctatgc cctcgcagaa gcaacggagc gcctcgccct cgctttcgcg gagagccgcc
     5281 agcgcccgga ggatacacac gtgcaggcca agtgccgata ccttgccggg caggtggctc
     5341 actacgccca ggatctctgc cagccgctgc atacgacggt ccaccacgat ggcaaggtgc
     5401 gcccggatgg cagttcgccg cgcaccggca tccactcgcg catcgatgac ctcattacgg
     5461 cgctccgcgt tgagccaagc gccctggctg cgcgccaaca ggtcgcgccc gtggaatcgc
     5521 tcatgccggc catcctgtcg gagctgaagc ggagcaatgc gctggttacg cgtgtctata
     5581 tgctggagcc gaagttgcgc gtgaaaggag cgcgcggttt ggcgccggaa gtctctgact
     5641 ttgcacagga gcgcgcgcgg gcgagcgtcc gcttcaccgc ctcgctctat gccaccgcat
     5701 ggaagctttc gggcacgctc cagctgccaa aagcggccgc ggagtagtgt ccagggcgaa
     5761 gatatccgcg tgattgagag caagagagag cggcaggatt ccgccacggg gagcgcgaaa
     5821 aaaacgagcg cggcatcctg ccgctgagga ctggctcgag taggacaaca aacagacacc
     5881 aggcactcta ccacttttca ggactccatt ccaccacgac tccactccgc tctgctacga
     5941 atgagaacat gacgagccgc atgagcccac atacccgctg ccctgttccc ccagcgaccg
     6001 gcaagggtca acacgcgcgt gctaccgcgc tgcctattcc cccagaatgc ctgtcttcgc
     6061 aggagcacgc gggtcatacc cgcactcacc gggctcccac cgacggatgt cggatggagc
     6121 atcggtaata cccggaggct gtgtgattca gaccgataag ctcttcgtcc atggaacgct
     6181 ggcaacgcca agacacctgc gcgtgctcac gggatttgaa ccgccccgac ggccggcgct
     6241 cctgcatgat ttccggcgag tgcccacgcc acacgccctg ccccacattc tgccagagcc
     6301 gggcgccacg accgaaggct acctgatcga gggaatcgat gccgaagtgt tggcgcgtct
     6361 cgatcggtat gaggagaatg gctcgctcta cgagcgccgc ccggttgtgg tgcgtgccgg
     6421 cggcgagtgg atccagacgc acgcctatgt gggaagcccc accgccatcg gggtacatcc
     6481 gtgccgcgat ttcgaaccgg aggatcgcgt ggagtaccac ctcctggccg agatggaaag
     6541 cacgctcgcc cgccgcttcg ggagcgcgga gagcgacatc cgccttggcc gggagctgtt
     6601 gggagatgcg atcgccgaac tcacccgaaa gcactggctc gacccgcggt ggtcgccgcg
     6661 ggccctcggg cgcgcgctgc aacacgcccc cctcccgagc ctggagtggc ttcgagagga
     6721 tgcgcacgcg cgccgcttcg ccagcgccta cctgcgcctc atcgcgcgca tcgtcatcct
     6781 caaccaggtg gaggaccgga ttcgctcgga gttccggcag gcgctccggc cgcaagcctc
     6841 ctgctatccg cgcaccgaat cgttgctggc ggcgctcgcc ttcctcaatg cgcgtgcgga
     6901 agccctcgag gcggcactcg cgggggccgg cctccgcgtc taccaggccg actatgatta
     6961 tgtcgacacc gtgcgccagg cgatcctcct ggccgacgcg ctctatgatc gaagccttgc
     7021 ggcggagatc atcgcgcgca tggagatcaa ccgtcgccct gggggcacgc ccctcggcgc
     7081 ggagctggag ttcagccccc tgggagcgcg cgcgattcgc tcgcggcgcg gcgccgaccc
     7141 gcagctcgat gggctctact acttccacga tttcgacctg gcgcgccgcc tctggaagct
     7201 gggtgggtat cgcgatgacc acaccggcgt caccttcgat gccgggcgaa cacgcggttt
     7261 cctggagatc gccatcggtc ggctgcaggc ggaaggcgat ctctcgcagc ctgccaccac
     7321 tgacctctgg attctctcgg agctgatccg agagacggtg gagttcatcg gcatcccacc
     7381 gcacagcctg catctctctc tgcaggccga ggaaggccgg cctttcaccc gtctcccggg
     7441 tccggagttc ctcttctgcc tcctcctctt ggggggtgac ctgggtctcg acaccgtcgg
     7501 catccttcgc gagaagcgga tccacggggg agagattcgc acgcgtcgtg gagagctggc
     7561 gttctcgcgg tggaacgccc accgcgccgt ggagggcggc cagccacccg tgccggtgat
     7621 agagttcgcc ttcccgcgcc tggcggcggg gcgccactac gagccgctcc tgttcgcact
     7681 gaaaggactc cagcgcgcgc tgaaccccct ccctctggta ggccccaatg gccccctgga
     7741 tcggcgttat taccgacaga tggagcaggc cctccttcag tgggcagcgg cgccaacgcc
     7801 ggtcgatgag gggatcatca cggagttcat cgacgttgtg gccacgggcc tacgcatgga
     7861 gcgcgaagcc ggccatggcc attcgccggg cgagtgcgcc gatggcctta ttcgcgtgga
     7921 ggccgcgctg catgagcgca acgaggagat ccgccggatc tcctcgcagg ggcttgcctg
     7981 cgcccccact cagggggcgt aggctttcgc gcgttcacga ggcaggccac ccacgtgacc
     8041 tgccccgtga acgccatctg ttccgttcgg agcgtgtcag cctgttcttc gcctgcttct
     8101 gtcgatgttg ctctcggttt cgacatccac gcccttggtt tcgacgtcga tgtcgccctc
     8161 cttatgcacc tcggcaacct cgcggcggat cgtctcggta cgccgctcca tctctgtctc
     8221 tggatgctgg cgaacgatga tctcctcctt gacgacgggg cgcttctcga tatgcacttc
     8281 ctcttcgcgg accgggatgc gcacctcgcc ctcggtgatc tcgccttctg tgtggtagcg
     8341 cgcctctccc gtcaccggcc ggcgctctac ctctacctcg gtatgcgaca caggcacctc
     8401 aatggtgcgc tgctcggaga caacgtgctt ctcgacctcg atctcgccgg tatggacctg
     8461 gcgggttgcg accatctcct cctcgcggag gggtacgcgg gtctcgcccg cggggacctc
     8521 gccgccgcgc atgccggcca tctcatgcgt ttcggcacgc ccgggcatgc cctcttccat
     8581 ccagtagcgg tacgccccgg cgtagtcgat ctcctcgcca gcgcgccact cgatcgcgtt
     8641 ttccaatcga tcgcgcgggg cgtcgattct cacctgatgg ttttcgcggt tgaggtggat
     8701 gcggtccaac ggcaccaaca cgtgccggcc gcccatgccc caaaggccac cgatctgggc
     8761 aaggatgaaa tagacatgtt gggtcttcgg actggcgatc aggtcgtgaa ccttgccaac
     8821 gtgatcgccc tcctgcgtga ggatatccca tccccgaatg tcgggctcac cctcggcgat
     8881 ggtccagtgc tccaggtctt ccatccgctg caggtccaac tcctcccggc ggtaggtgcc
     8941 ctcccggagc atgcggaacc ggtctccctc accgagcgga tagggctgat gcccacccat
     9001 ctcgtttgcc atctctcatt tccctcccgg cgcaatggcg ccgcgcctca cgtggcgtcc
     9061 caagcgctct gggctccttt gccgctggga ctgccagtct gatcactatc gctctctgcc
     9121 atgctacggt acccgcggcg gcgcagccac aaaccacgcg ccggcgcgac cattcgcgcg
     9181 acacgctctg cttgacaagc acgccttttc caggtaatct agccgtgctg ctgtcaatcg
     9241 ataccagccg aggaggagac tgccatgttc aaccgtctgg tcgcctttgc cgtcctctgc
     9301 gccgtggcga cgctgcctgg cgcccggctc ttcacggtgg atgccagcga accactcgtt
     9361 tttgaagcgg aggcatacac cacgccggcc gatgcctggg agaagaaccg cctcaccgag
     9421 ccgaaatg
//
LOCUS       DAOSCV010000032         9387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4811_length_9387_cov_3.502357, whole genome shotgun sequence.
ACCESSION   DAOSCV010000032 DAOSCV010000000
VERSION     DAOSCV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4811_length_9387_cov_3.502357"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="ftsA"
                     /locus_tag="PLY56_01555"
     CDS             complement(<1..876)
                     /gene="ftsA"
                     /locus_tag="PLY56_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ20194.1"
                     /translation="MAKSEIIVGLDVGTTKVCAVVGEVTKNGVELSGHGISPCQGLQK
                     GIVVDIDATVLAIEEAIEMAQQATGFQIRSVAVGITGQHIQSFNSRGSIEIERFRDIT
                     AEDVEQAKEAAQRIDIPAGREIIHSIPRAFRIDGHEGVRRPIGMTGTHLEVEMHIVTA
                     ETSFLQNLIKCVHQADLSIIPGGIILEPLATAEAVLSAEERELGVALVDIGGGTSDVA
                     LFRGGGVFHTSVIPVGGNHVTNDLAVGLRVARNEAERIKLEHGTALDEGIEELEAITV
                     TPLGQPADETVRIPKR"
     gene            complement(938..1297)
                     /locus_tag="PLY56_01560"
     CDS             complement(938..1297)
                     /locus_tag="PLY56_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HOJ20195.1"
                     /translation="MWLAPACGLIGGVLIALALPWHVPIQYANYLSVALLAGLDTILG
                     GYRAGLEERFDDHVFVTGFFINMLAATALVWLGDLIGLRELYLAAVVALGVRMFTNLG
                     IVRRLLVARMRVFRETP"
     gene            complement(1297..2103)
                     /locus_tag="PLY56_01565"
     CDS             complement(1297..2103)
                     /locus_tag="PLY56_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOJ20196.1"
                     /translation="MAVGDLHKIEVHRKHSRHWLWYVTAISFVTGALLAACLRTQRQW
                     RADLGIGVWRPEIIAEHLKASREEAKGYKAELAATRGRVTELENTLAQGGRDKELFNQ
                     ELQKYKMLMGLVELEGPGIIMRLDDSTLRSATSDPEEIESLLLHDYDLQRVVNELRVA
                     GAEAISLNGQRIVERTAIRCVGPVAHVNNVPIGTPYEITAIGDPQVLRSGLTIRGGIV
                     DWLKTLGFPVELKAMPKVRVPAYTGPIELRFAKPVSASKQEADTAKGGKQ"
     gene            complement(2075..2980)
                     /locus_tag="PLY56_01570"
     CDS             complement(2075..2980)
                     /locus_tag="PLY56_01570"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ20197.1"
                     /translation="MRRPTHPRRYPRHPLDEDLRVSRRRLGRPRRNRRRRRIPLLVWL
                     LAILTVEVVAVGLKDPRFNLARIEVQGVEVLTEEQVVRRSGLRPGKNLFQIRLKEARQ
                     RLLEVPAVADVTLHRRLPNRVLVRVHERQAMACVAAKNALFTIDATGIAFRKDAKPPR
                     GLPLVTGLETKPLQLGGRIDAERARELREFVEAAAEYPQRGVPRISIDQNGNLCFNSA
                     ELGYQVRLGPAVQLKEKLALLAALERSLPDIRDRCQYIDLSCLEAPAWKPKKVALATN
                     DHAGESRPVAPSGETLHGRGGFAQD"
     gene            complement(2992..3936)
                     /locus_tag="PLY56_01575"
     CDS             complement(2992..3936)
                     /locus_tag="PLY56_01575"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOJ20198.1"
                     /translation="MSDGRRLRVAVLMGGMSAEREVSLKTGAKVVQALDPTRYEVFTV
                     DPRPRDEGCEWLETLARAQVDVAFIALHGRFGEDGTIQGLLELLGIPYTGSGVLSSAL
                     AIHKARSKQIYRSAGIPTPESVELDSSMDPVALREWMDTFPLPAVVKPSREGSTIGIT
                     IVRSRDQMAPAVELAFRHDPSVLIERFVSGVEITGAVLGNDHPQALPLIEIVPRSGFY
                     DYEMKYTPGATEEIVPARISPEQTEQAQNLAIQAHLALGCRGVSRTDMIVAPDGIWVL
                     ETNTIPGMTETSLLPRAAASVGISFPSLVERIIAHALE"
     gene            complement(3975..5018)
                     /locus_tag="PLY56_01580"
     CDS             complement(3975..5018)
                     /locus_tag="PLY56_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HOJ20199.1"
                     /translation="MFQNKRLLIYAPDEFGRGHSKTAEGVLRYGRNPVVGIVDASRTG
                     TCADVIGIRADVPFYPDIASALAAGPEALLLGVAPRGGRLPASWRNDIAFALRSGLDI
                     ISGLHEVLAADPEFRALAEEHGRQIWDVREPPADLPVGNALVCGLSEQVVLTVGSDCA
                     MGKMTASLEIEKSARKRGRNPIFIPTGQTGIMIAGFGISIDRVIGDFMAGATEKLILE
                     HGPGHDLILVEGQGSLLHPGYSGVTLGLLHGAMPTEMILCHEPSRKFIRDSTLPIPPL
                     PEVIALYEAMTLPLRKAKVVGIALNCFDLDEEETRRQIEKTQELTGLPTTDVVKFGAD
                     CLAEAIDKARERR"
     gene            complement(5140..5754)
                     /locus_tag="PLY56_01585"
     CDS             complement(5140..5754)
                     /locus_tag="PLY56_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOJ20200.1"
                     /translation="MLHRGQPYLRDVILAQPGRYVLLSALAAIALARHLGIAEQSVRE
                     ALATFEGARRRLEMRGEVGGVTVVEDFAHHPTQARTSLETASVRFPDRRLWCVYDLHT
                     FSSRNRRYLPDYAQAFGQAHGVVLPAMHHPEQIPEAQRMSIPELTEAIRQTQPNVAYI
                     PNRPEIVTYLADRAQPGDVVLFMSSGGLEDLIEGLLETLRQRAG"
     gene            complement(5685..6545)
                     /locus_tag="PLY56_01590"
     CDS             complement(5685..6545)
                     /locus_tag="PLY56_01590"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOJ20201.1"
                     /translation="MKRVHIVSICGVAMSALAKILKDRGYSVTGSDEDVHPPVTDLLT
                     EYGIPYVEGHRAENLGDPLPDLVVVGNHAALDNPEVVAAREAGLPILSLPQMLHELLA
                     DYEPVVVTGTHGKTSTCSLLAWMLERAGLDPSFMVGGIPRNLGVNTKLGSGKLFLLEG
                     DEYLSAKFDRTPKFLYYRPRIGIVMNVALDHINFFSGQEEYIDAFKRFAALVPADGLL
                     LAGIDSPNVASVAAAATSRSKPLALPATPSGRRPTFAWSRNGAFSTCCTAGNPTCVMS
                     SWHSRVATYS"
     gene            complement(6542..7654)
                     /gene="murG"
                     /locus_tag="PLY56_01595"
     CDS             complement(6542..7654)
                     /gene="murG"
                     /locus_tag="PLY56_01595"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ20202.1"
                     /translation="MKIFLTGGGTGGHVCPAIAIAEALKQAGHEVLFVGTRGGLEARA
                     VPAAGYPIVHLPSRPVSRKLSPDALLSLAVTGAGVVAAARLLARERPNAIASTGGYAG
                     AALGFAAAILRVPALLFEPNAIPGRTTLLLSRWARRIAVAYAECGPRFGAQAEARVVL
                     TGTPVRPDIGGGDPVAAREAFGLDPNRPTLLVVGGSAGARSINNAVIAAAPALLDAGA
                     QILHQTGRANIEGVKAATAHLAGRGYVPVDYLENMGTAYAAADLILCRSGASTIAEVT
                     TCGLPAVMVPYPYAVGDHQTYNARALADAGAGRIIPDRELTPEGLADAVLFYLNNREA
                     CQRASAASRSLARPDAAASIVRLLEEMGGTMIQSQL"
     gene            complement(7590..8789)
                     /gene="ftsW"
                     /locus_tag="PLY56_01600"
     CDS             complement(7590..8789)
                     /gene="ftsW"
                     /locus_tag="PLY56_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOJ20203.1"
                     /translation="MVSERQSLDTSLLVATAILVACGILFIYDASFVRASQTHYTNFD
                     PEFYLKRQFVWACVGTVALITAIAVPYWKLDRLAVAGMAITIGLLILVLFAGHESHGS
                     RRWFILGPLRIQPAEFAKIAVVLYLAQWMSVRKQKIRSLTEGFLPLAGLLGLLGVLVA
                     IEPDLGTALVIVCTGTVMLFVGGARKRHLLMLGLVGVLAVVVLIWMEPYRWDRVLDWL
                     NPERDVQGSGYQVVQSLIALGSGGLVGVGLGQGRQKYFYLPAEHTDYILGTIGEELGF
                     LGTMGLLFLFLYVAVRGFTIAHRTKDPFGSLIASGITTMITGQALLNIGVVTKTLPAT
                     GVPLPFISYGGSSLVLTMVAMGLLANISQYPSASPGTARGNKGSNEDLLDRRWDRRAR
                     LSSHRHR"
     gene            complement(8812..>9387)
                     /gene="mraY"
                     /locus_tag="PLY56_01605"
     CDS             complement(8812..>9387)
                     /gene="mraY"
                     /locus_tag="PLY56_01605"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ20204.1"
                     /translation="KPLARLLSAPLAGVWPGAFGAAYVVLDVLFLLGLANAVNFSDGL
                     DGLAGGLTVIAALTAALLLPPGLQNTLGVFLFALAGGCLGFLWFNCNPARVFMGDTGS
                     LALGGALGAVAVLTRTELLFFIVGAVYWAELFSVILQVISFKTTGRRIFRMSPIHHHF
                     ELVGWKEGQIVTRFWLAGAATGALALLLRHF"
BASE COUNT         1725 a         3119 c         2945 g         1598 t
ORIGIN      
        1 gcgttttgga atgcgaacgg tttcatcggc gggttggccc aagggcgtca ccgtaatcgc
       61 ctccagctcc tcgattccct cgtctaacgc cgtcccatgc tccagcttga tgcgctccgc
      121 ctcgttgcgc gccacccgca ggcctaccgc gaggtcgttc gtcacgtggt ttccgcccac
      181 agggatcacg ctggtatgga acacccctcc tccccggaag agggccacat cactcgtacc
      241 acccccgata tcgaccagag ccaccccaag ctcccgctcc tcggcgctca gcaccgcctc
      301 ggcggtggcc aggggctcca ggatgatccc tcccggaatg attgagaggt ccgcctggtg
      361 gacgcacttg atcaggttct gcaggaagct ggtctccgcc gtgacaatgt gcatctccac
      421 ttccaggtgg gttccggtca tcccaatcgg gcgacgaacg ccctcgtgcc catcaattcg
      481 gaacgcccgg gggatgctgt ggatgatctc gcgtccggcc ggaatatcga tgcgctgtgc
      541 cgcttccttc gcctgttcga catcttccgc ggtgatatcg cggaagcgct cgatctctat
      601 gcttcctctc gagttgaacg actggatgtg ctgcccggtg atcccaacgg cgacggagcg
      661 aatctggaag ccggtcgcct gctgggccat ctcgatcgct tcctcgatcg cgagaacggt
      721 ggcgtcgata tcgaccacga ttcccttttg caggccctgg catggcgaaa tgccgtggcc
      781 ggaaagctcc accccattct tggtaacctc gcctacgacg gcacagactt tagtagtgcc
      841 cacatcgagg ccgactataa tctctgattt ggccaaggta tcccccaaag gccgtctgct
      901 tgaagccggg caggggctgg aaccgggcta cgggcgctca gggcgtctct cgaaacacgc
      961 gcatccgggc caccagtagc cggcgcacga tgcccaagtt agtaaacatg cgcacaccca
     1021 gggccaccac cgcggcgaga tatagctcgc gcaggccgat caagtctccc aaccagacca
     1081 gggctgtggc ggcgagcata ttgataaaga accccgttac gaaaacgtgg tcgtcaaagc
     1141 gttcttctaa tcccgcgcgg tagccgccca ggatcgtgtc caggccggca agcagcgcca
     1201 ccgagagata gttcgcatat tgaataggca catgccaggg aagcgccagt gcgatgagca
     1261 caccaccaat gagcccgcac gcgggcgcga gccagatcac tgcttccctc cctttgccgt
     1321 atctgcttct tgtttggatg cggacacggg cttcgcgaag cgtagctcga tggggccggt
     1381 ataagccggc acgcgcacct tcggcatcgc tttcaactcg accgggaagc ccagcgtctt
     1441 caaccagtct acaatcccac cccggatggt caacccactg cgcagcacct gggggtcgcc
     1501 aatcgccgtg atctcgtagg gtgtgccgat aggcacgttg ttgacgtgag ccactgggcc
     1561 cacgcaccgg atcgccgtgc gctccacgat gcgctgccca ttcagcgaga tcgcctccgc
     1621 gcccgcgaca cgcagctcgt tcacaacgcg ctgcaggtcg taatcatgca gaaggaggct
     1681 ctcgatctcc tcgggatcgg aggttgccga gcgcagggtg ctgtcatcca ggcgcatgat
     1741 gatgcccgga ccctccagtt ccaccaggcc catcaacatc ttgtacttct gaagttcctg
     1801 gttgaacagc tctttatcgc gtccgccctg cgccagagtg ttttcaagct cagtgacgcg
     1861 cccgcgcgta gccgcgagct ctgccttgta gccctttgcc tcctcccggg aggccttcag
     1921 atgctcggcg atgatctccg gacgccagac gccgatcccg agatccgcgc gccactggcg
     1981 ctgcgtgcgg aggcacgcgg ctaacagcgc gcctgtgaca aaggaaatgg ccgtaacata
     2041 ccaaagccaa tgtcgcgaat gtttacgatg cacttcaatc ttgtgcaaat cccccacggc
     2101 catggagagt ttcccccgat ggagcgacgg gtcgcgactc gcccgcatga tcgttggtcg
     2161 ccagggcaac cttcttgggc ttccatgcgg gcgcttcgag acaactcagg tcaatgtact
     2221 ggcatctatc gcggatatcg ggcaggctgc gctctagcgc ggcaaggaga gcaagtttct
     2281 cttttagttg cacggcaggg ccgagccgaa cctgatagcc tagttccgcg ctattaaaac
     2341 atagattgcc gttttggtca atagaaatgc ggggcacgcc acgctgtggg tattcggccg
     2401 cagcttccac gaactcgcgc agttcacgcg ccctctcggc gtcgatgcgg cctcccagtt
     2461 ggagcggttt ggtctccagg ccggtcacca acggcaggcc gcgcggcggt ttcgcatcct
     2521 ttcggaaggc gattcccgtc gcatcaatgg tgaagagggc attcttcgct gccacgcacg
     2581 ccatcgcctg gcgctcgtgt acgcggacaa ggacgcggtt cggcagccgg cggtgcagcg
     2641 tgacatcggc tacggcgggc acctctagca ggcgctggcg ggcttccttc aggcggattt
     2701 ggaagaggtt cttgcccggg cgcagcccag agcggcgcac cacctgctcc tcggtgagca
     2761 cctcgacgcc ctggacctca atccgcgcca ggttgaaccg gggatccttc aggccaacgg
     2821 ctaccacctc caccgtgaga atggcgagaa gccagacgag gagggggatg cgccgccgac
     2881 gtctgtttcg gcgcggccgg cccagacggc ggcgcgagac gcgcaggtcc tcatcgagcg
     2941 gatgtcgtgg gtagcgacgc gggtgtgtcg gtctcctcat gctccccctc ctcattcaag
     3001 cgcatgcgcg atgatccgct ccaccaggct cgggaatgag ataccaacgc ttgcggctgc
     3061 ccgaggcaac aggctcgtct cggtcattcc cgggattgtg tttgtctcca ggacccagat
     3121 cccatctggg gccacgatca tgtcggtgcg cgatacgcca cggcagccaa gggcgaggtg
     3181 tgcctgtatc gccaggttct gtgcctgttc tgtctgctcc ggcgagatgc gcgccggcac
     3241 gatctcctcg gtcgctcccg gcgtatactt catctcatag tcatagaagc cactgcgcgg
     3301 cacgatctcg atcagcggca gcgcctgcgg atggtcgttc ccaagcaccg cgccggtgat
     3361 ctcgacgccg gagacgaagc gctcaatcag cacgctcggg tcatgccgga acgccagttc
     3421 caccgcgggc gccatctgat cgcgagagcg cacaatcgtg atcccgatcg tcgagccctc
     3481 gcggcttggc ttcaccaccg cggggagggg aaaggtatcc atccactcgc gcaaggccac
     3541 cggatccatc gaggaatcga gctccacgga ttcgggcgtt gggatccccg cgctccggta
     3601 gatctgcttg ctgcgcgcct tgtggatcgc cagagcactg ctaagcacgc ccgacccggt
     3661 gtaggggatg cccagcagct cgagcaagcc ctggatcgtg ccatcttcac cgaaacgccc
     3721 gtggagcgcg atgaaggcta catccacctg cgcacgcgcc agagtctcca gccactcgca
     3781 tccctcgtcg cgtggccgcg gatccaccgt gaagacttcg tagcgcgtcg gatcaagtgc
     3841 ctggacaacc ttggccccgg ttttcagaga cacttcacgc tcagccgaca tccctcccat
     3901 cagcacagcc acgcgcaacc gccgtccatc agacatcctc acgcctccct cacccatacc
     3961 cccacgttct cctgtcagcg tctttctcgg gccttatcga ttgcctcggc caggcagtcg
     4021 gcgccgaact tcaccacgtc cgtcgttggc aggccggtca gctcctgcgt tttctctatc
     4081 tgccggcgag tctcttcctc atccaggtcg aagcagttga gtgcgatccc gaccaccttc
     4141 gctttccgca aggggagcgt catcgcctcg tagagggcga tcacctctgg caagggtgga
     4201 atgggaagcg tcgagtcgcg gatgaacttg cgcgacggct cgtggcaaag gatcatctcg
     4261 gtcggcatgg cgccgtgcag cagccccagc gtaacgccgg agtagccggg atggagcagt
     4321 gagccctgcc cctcgaccag gatcaggtca tggcccggac catgctccag gatcagcttc
     4381 tcggtggcgc ctgccatgaa gtcgccgatg acgcggtcga tggagatacc gaagccggcg
     4441 atcatgatcc ccgtttgacc ggtggggatg aagatcgggt tccgtccgcg cttgcgcgcg
     4501 ctcttctcga tctccagcga ggccgtcatc ttgcccatgg cgcaatcgct gccgacagtg
     4561 agcaccactt gctcggagag gccacacaca agcgcgtttc cgacgggaag atccgcgggt
     4621 ggctcgcgca catcccaaat ctgccggccg tgttcttccg cgagcgcccg aaactccgga
     4681 tcggcggcga ggacctcgtg cagcccgcta atgatatcaa ggccgctgcg gagtgcgaac
     4741 gcgatatcgt ttcgccacga ggcgggaagg cggccgccgc gcggcgcgac gccgaggagc
     4801 agcgcttctg gccccgccgc gagcgccgag gcgatatcgg ggtagaaggg aacatccgct
     4861 cgaatgccga tcacatcggc gcatgtgccc gtgcggctcg catcgacgat gcccaccacc
     4921 ggattccgcc cgtagcgaag cacgccctcc gcggtcttcg agtgccctcg cccgaactcg
     4981 tcgggcgcat agatcaggag tcgcttattc tgaaacatgc tcttcctccg atggggcagg
     5041 gggctctctc ccactgccct cagtcatcgg cgacccgtgc tccgtaacgg atcgccgaac
     5101 accgcgttgc cggtgcgctc cgggccgttg tcggcgtcgc tacccggccc gctgccgcag
     5161 tgtttccaat aggccctcga tcaggtcctc caggccgccg gaggacatga agaggacgac
     5221 atcgcccggt tgagcgcgat ccgcgagata cgtgacgatc tccgggcggt tcgggatgta
     5281 ggcgacgttg ggttgcgttt gccggatcgc ctcggtcagt tccgggatcg acatgcgctg
     5341 cgcttccggg atctgctccg ggtgatgcat cgccggcaga accaccccat gtgcctgtcc
     5401 aaatgcctgc gcgtagtccg gcaggtagcg ccggttccgg ctgctgaaag tgtgcaggtc
     5461 gtagacgcac cacagccgcc ggtccgggaa gcgaacagag gcggtctcca atgacgtgcg
     5521 cgcctgcgtc ggatgatggg cgaaatcctc gacgacggtc actccaccca cctcgccgcg
     5581 catctccagg cgccggcgtg ccccctcaaa ggttgccagc gcctcgcgca cgctctgttc
     5641 agcgatgccg aggtgccgcg ccagagcgat agccgccagg gcgctcagga gtacgtagcg
     5701 acccggctgt gccaggatga catcacgcag gtagggttgc ccgcggtgca gcacgtcgaa
     5761 aacgctccgt tccggctcca ggcgaacgtt ggccgcctgc cactcggcgt cgccggcaaa
     5821 gccaaaggtt tggaccggct ggtcgccgcc gcggcgaccg atgctacgtt gggcgagtcg
     5881 atcccggcga ggagcaggcc atccgccggc acgagcgcgg caaagcgctt gaacgcgtcg
     5941 atgtactctt cctgaccgct aaagaagttg atgtgatcca gcgccacgtt catcacgatc
     6001 ccgatgcgcg ggcggtagta gagaaacttc ggcgtgcggt cgaacttggc gctcaggtac
     6061 tcgtcgcctt caagcaggaa gagcttgccg gagccgagct tagtgttcac ccctaggtta
     6121 cgcgggatgc cgcccaccat gaagctggga tccaggccag ctcgctccag catccaggcg
     6181 aggagggagc aggtggatgt cttgccgtgc gtacccgtga cgacgaccgg ctcgtaatcg
     6241 gccagaagct cgtgcagcat ctgggggagc gagaggatcg gcaggcccgc ctcgcgcgcg
     6301 gccacaacct ccggattgtc cagggcggcg tggttgccta ccacgaccag gtcgggcagc
     6361 gggtctccca agttctctgc ccggtggccc tcgacgtagg gaatgccata ctcggtgagg
     6421 aggtccgtga cagggggatg cacatcctcg tccgagccgg tgacggagta gccgcggtcc
     6481 ttcagtattt tggcgagggc gctcatcgcg accccgcaga tggagacgat atgcactcgt
     6541 ttcatagctg cgattgtatc atagtaccgc ccatctcctc cagcagacgg acgatagagg
     6601 cggcggcgtc gggccgggca agcgagcggc tggcggctga ggcgcgctgg cacgcttccc
     6661 ggttgttgag atagaagagg acggcgtccg cgaggccctc cggggtcaac tccctgtcgg
     6721 ggatgatccg gccggcgccg gcatcggcca gggcgcgcgc gttgtaagtc tggtgatcgc
     6781 ccacggcata ggggtagggc accatcactg ccggcagccc gcaggtggtc acctccgcga
     6841 tggtagacgc gcccgagcgg cagaggatca gatccgcggc ggcataggcg gtccccatat
     6901 tctccaggta atcgaccggc acgtaaccgc ggccggcgag gtgtgcggtg gcagccttca
     6961 ctccctcgat gttggcgcgc cctgtctggt gcaggatctg cgcgccggca tccagcagcg
     7021 ccggtgccgc cgcgatgacg gcgttgttga tcgagcgcgc gcctgcgctg cccccgacga
     7081 caaggagcgt aggccggttg ggatccagcc cgaaggcctc ccgagcggcg acgggatctc
     7141 cccccccgat atccgggcgc accggcgtgc cggtgaggac gacgcgcgcc tcggcctggg
     7201 ccccaaagcg cgggccgcac tcggcgtagg caacggcgat ccgacgcgcc caccgtgaga
     7261 gcaggagggt ggtgcgcccc ggaatcgcgt ttggctcgaa gaggagcgcc ggcacgcgga
     7321 ggatggcggc agcgaagcca agggccgcgc cggcgtagcc gccggtgctc gcaatcgcgt
     7381 tcggacgctc gcgcgcaagg agacgcgcgg cggcgactac ccccgccccg gtaacggcca
     7441 gcgagagcag tgcatccggc gagagcttgc gactaacggg ccggctgggc agatgtacga
     7501 tggggtagcc ggcggccggc acagcgcgcg cctcgagccc gccgcgcgtg cccacaaaaa
     7561 gaacctcgtg cccggcctgt tttagcgcct cagcgatggc gatggctgga cagacgtgcc
     7621 cgccggtccc accgccggtc aagaagatct tcattcgaac ccttattccc tctcgcagta
     7681 cccgggctcg cgctcggata ctgcgagata ttggccaata ggcccatcgc aaccatggtc
     7741 aagacaagcg aggaaccgcc ataggagatg aaggggagcg gcactcctgt cgcgggcagc
     7801 gtcttggtga cgacaccaat attcaggagc gcctgccccg tgatcatcgt cgtgataccg
     7861 ctcgcgatca ggcttccaaa gggatccttg gtccggtgcg cgatcgtgaa cccgcgcacc
     7921 gcgacgtaga ggaagaggaa gagcagcccc atcgtaccca ggaaaccaag ctcttctcca
     7981 atggttccaa ggatgtagtc cgtatgctcc gcggggaggt agaagtactt ctgccttccc
     8041 tggcccaggc caactccaac caggccgccc gagccaagag cgatcagcga ttgaaccacc
     8101 tggtacccgc tgccctgcac gtcgcgctct gggttgagcc agtcgaggac gcggtcccac
     8161 cggtagggtt ccatccagat cagcacgacg acagccagca ctccgacaag cccaagcatc
     8221 agcaagtggc gtttgcgcgc gccccccacg aagagcatta ctgtaccggt gcagacgatc
     8281 accagcgccg tgcccaggtc gggctcgatg gcgaccagga ctcccagcaa acccaggagc
     8341 cccgccaacg gcaggaagcc ctcagtgagg ctccgtatct tctgcttgcg cacggacatc
     8401 cactgcgcca ggtagaggac aaccgcgatc ttcgcgaact ccgcgggctg gatccgcagc
     8461 ggccccagga tgaaccagcg ccgtgagccg tgggactcgt gccccgcgaa gagcaccagg
     8521 atcaggagcc caatggtgat cgccatgccc gctaccgcca ggcggtcgag cttccaatag
     8581 ggcacggcaa tcgctgtgat gagcgccacg gtacccacgc aggcccagac gaactgccgt
     8641 ttcaggtaga actccgggtc gaagttggtg tagtgggtct ggctcgcgcg cacgaagctg
     8701 gcatcataga tgaagaggat gccacaggcg accaggatcg ccgttgccac cagcaaactc
     8761 gtatcgagag actgtctctc ggaaaccatt cacaacctcc ggtgccacgg ctcagaagtg
     8821 gcgaagcagc agcgccagcg cgccggtagc cgcgccggcc agccagaagc gcgtcacaat
     8881 ctggccctct ttccatccaa ccaactcgaa atggtggtgg atgggtgaca tccggaagat
     8941 ccgccgtccg gtggtcttga aggagatcac ctgaaggatc accgagaaga gctctgccca
     9001 gtagacggcg ccgacgatga agaagagcag ctccgttcgt gtcaacaccg cgacggcgcc
     9061 cagcgcgccc cccagagcaa gcgacccggt atctcccatg aaaacccgcg ccggattgca
     9121 gttgaaccac aggaagccga ggcagccgcc cgcgagggcg aagagaaaga cgcccagtgt
     9181 gttttgcagc cccgggggga ggagcagcgc cgccgtcagc gccgcgatca cggtcagccc
     9241 gccggccaat ccatccaggc cgtccgagaa gttcacggcg ttggccaggc cgagcaggaa
     9301 gagcacatcc aggacaacat aggccgcccc gaaggcaccg ggccacaccc ccgccagagg
     9361 cgcggagagc aggcgagcca gaggctt
//
LOCUS       DAOSCV010000033         9344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4831_length_9344_cov_3.063408, whole genome shotgun sequence.
ACCESSION   DAOSCV010000033 DAOSCV010000000
VERSION     DAOSCV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9344
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4831_length_9344_cov_3.063408"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..953
                     /locus_tag="PLY56_01610"
     CDS             264..953
                     /locus_tag="PLY56_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ20205.1"
                     /translation="MRLTPTDRDRLLIFTAAELARCIPGPEAQLRVIYHGVSESWFHV
                     EKGAQPHERPYLLYVGNVKPHKNLRRLLNAFELVRDRIPHDLLIVGQREGFITPDEAV
                     GAKALELGNRVHFTGHVDNATLQQCYAHADALVFPSLYEGFGLPPLEAMACGCPAAVS
                     RAASLPEVCGDAALYFDPHNESDIAEKIERIVSDVELRSELVARGKERAHSFRWQDTA
                     ASVWNVIGWRA"
     gene            1030..2499
                     /gene="wbaP"
                     /locus_tag="PLY56_01615"
     CDS             1030..2499
                     /gene="wbaP"
                     /locus_tag="PLY56_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HOJ20206.1"
                     /translation="MEEVTASQLIRIDEAEAQIAEAAAAARPRCTAVILALADLISLL
                     AAWGISVSLAHLLHTGPTPDFYWALLPAAVIFLAVYAAFGLYPTVGHGPAEELKRTSL
                     ATTWAYTISATAAFLTRTEAPCPAGVIIAAWVLSLALVPICRSVARALYARQRWWGHP
                     VVIFGAGRTGALIIRALRDCPDLGLNPIAVVDDDPARWGRLDGTPVVGPLEKAVTLAK
                     AHSIRYAIVAMPGVARERLLSIIEQYGKPFRHVLVVPDLYGIASLWVVGRDLGGMLTL
                     EVRRRRLMPIHKAIKRALDIVLVLLSSVVVLPIFALIALAVRLDSPGPILYGSIRIGR
                     NGRRFTAWKFRSMVTNADEVIQEYLRQNPKEREEYERVHKLRQDPRVTRVGRFLRRTS
                     LDELPQLWNVLLGDMSLVGPRPYAEYDFRDDNLEEFDLYYQVLPGLTGLWQVSGRAET
                     TFAERIAFDNYYVRNWSLWLDLYILARTVWVVLFGRGAY"
     gene            2548..3723
                     /locus_tag="PLY56_01620"
     CDS             2548..3723
                     /locus_tag="PLY56_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ20207.1"
                     /translation="MRVAIVHDWLTTYAGAEKTLEQMLAVFPSATLYSLLDFMPPDQR
                     RFLRDRPVKTTFIQRMPLARRNHRVYIPLMPLAIESLDLSGYDVVLSSSYAVAKGVLT
                     GPDQLHVSYCYSPMRYAWDLQHQYLGESGLRSGVRSLLARILLHYIRMWDVRTAAGVD
                     HFVAISDFIARRIRKVYRREAAVIYPPVDVSARKVRTEKEDFYVTVSRLVPYKKVPLI
                     IEAFSRMPEKKLVVIGDGPELGKARACAGPNVQVLGRQPYEAVNDAIAKARAFVFAAE
                     EDFGIAPVEAQACGTPVIAYGKGGSTETVVPGKTGLFFSEQTVEAICDAVQRFEACRS
                     DFDPAQIRANAERFSDDRFREELGRFVHSAWAAFQAGSRGSAVHEDSLVPTGRDVMA"
     gene            3803..4864
                     /locus_tag="PLY56_01625"
     CDS             3803..4864
                     /locus_tag="PLY56_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ20208.1"
                     /translation="MKILITGGAGFIGCNAARHFMQRGHEVVVFDNLSRKGASANLEW
                     LRSQGRFAFIAGDVRDSRQVMDAVGGCDVVLHLAAQVAVTRSIVDPREDFETNALGTL
                     NVLEAARLATHPPIVLFSSTNKVYGDMANTPIVEESDHYRLADLPFGVSEEYPLNFLS
                     PYGCSKGAADQYTRDYARIYGLRTVVLRQSCIYGTRQLGVEDQGWVAWFLAATLLDRP
                     ITLYGDGKQVRDLLFVDDLVELYERVIERIDCVSGEVFNVGGGPANQLSLLQCLEQIR
                     KVTGKEPAISFAPWRPGDQRVFVADVRRASDMVGWQPKTGVAAGIAALYEWASQNRDL
                     LASVLSEPSPGRPGSMGRN"
     gene            4868..5584
                     /locus_tag="PLY56_01630"
     CDS             4868..5584
                     /locus_tag="PLY56_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOJ20209.1"
                     /translation="MTGKLRAAILAASIIQGTAACAAPAASQATAPPPISLSGSSGLV
                     RIPSADVLPYGDYSLGVNFLEAKYRSYWDSDTMLHFASVAVFPQVEVTARITNIDGRL
                     GVQYYDLLPGQPFGGWNFDRAFGVHVQLLKQSARVPVSLAVGDQDVTGNTAFGARYFV
                     ASHRQPRYGIHLGTGNGFLGKLFAGVDYQPHPKVRIAIDHDGNRANLGCTIQIQRLLL
                     MPTLSGFRSLGGGIAYWQRM"
     gene            5614..6264
                     /locus_tag="PLY56_01635"
     CDS             5614..6264
                     /locus_tag="PLY56_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ20210.1"
                     /translation="MGARPPGLLDARNRAVKRVIDYLLGLPAFLLSLPVIAFFALWIK
                     ATGHGSAFYSQEREGIDGTRIRVWKLRTMYPDAEATLRRYLDENPEADAEWQRYCKLR
                     NDPRVLPGLGSFLRRTSLDELPQLWNVLRGDMSLVGPRPYPDYHLDRFHPEFRALRRS
                     VLPGITGLWQVTTRSEGGLDVQQALDTRYILGWSIWLDLVILLRTIPCVLSRRGAW"
     gene            6687..7175
                     /locus_tag="PLY56_01640"
     CDS             6687..7175
                     /locus_tag="PLY56_01640"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ20211.1"
                     /translation="MLERLRVGGRYWLPPLLWTVLVLAFSTASFSGEQTGGILERVLG
                     LLWPAGLDPWQLEWVHLGIRKLAHFLEYAGLAFLWSLGFSRAGGYPGQAAFQMALLAS
                     ALAAVIDEAHQAVCPERTGALADVLLDCMGAVSGGAIYRVWERRHAKCLTSDSLNSSS
                     SW"
     gene            7133..7402
                     /gene="tatA"
                     /locus_tag="PLY56_01645"
     CDS             7133..7402
                     /gene="tatA"
                     /locus_tag="PLY56_01645"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ20212.1"
                     /translation="MPNIGFPELVVLLVIVLLLFGPKKLPDFAKSMGQAIREFRRASQ
                     EVVDDIRKAADETPAASESGRNAAYAQTAASEVERPAAEPSQKQL"
     gene            7399..8517
                     /locus_tag="PLY56_01650"
     CDS             7399..8517
                     /locus_tag="PLY56_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ20213.1"
                     /translation="MNRPLEIRPLTLLALLAAFYLGGLFGPARRQTEDRVPPAPAPLS
                     TAQAVAAPSAVVRNDAIADAAEKAGPAVVRIDTLTVQRDPWEELFGPDPFFGPRGRVS
                     SGTGSGVLISPEGYILTNEHVISGASQITVWLADGRQLKGEVVGGDSPSDLAVVKVPP
                     KDLPDPPRISTAPLRPGQWAIAIGNPFGLEHTVTLGVISATKRPMQIEDRTYDNLIQT
                     DCAINPGNSGGPLIDINGDVIGINTAILANGQGLGFANPIGFAKSVADELIRYGKVKR
                     PWPGLYVRTITPGLAARLGRRDTRGALVIGIIRNSPAHVAGLRRGDIILKVDGRDVTN
                     GDELMLDLKKRRIGDQVKLLIERDGYTGTTEMEIGEAP"
     gene            complement(8620..9339)
                     /gene="rplA"
                     /locus_tag="PLY56_01655"
     CDS             complement(8620..9339)
                     /gene="rplA"
                     /locus_tag="PLY56_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ20214.1"
                     /translation="MLGRSWSALQRYQESAAQVDKSRIYDPVEAIEIVKKIANAKFDE
                     TVDLAVKLGVDPRHGDQMVRGVTPLPHGSGKKRRVAVFARGENAQAAEEAGADAVGAE
                     DLVAKIQAGADKEFDVFLATPDVMGIVGRLGRVLTQRMPNPKAGTVTPDIARAVREVK
                     TGGRVEFKVEKTGIVHMPIGKASYTVDQLRENFSAAIGAILKARPAAAKGHYLQQIVI
                     SSTMGPGLRIDTAAAARIADK"
BASE COUNT         1546 a         3021 c         3032 g         1745 t
ORIGIN      
        1 aggccggccc gcgccggcgg tggcatcgat acacaggtcc ttgaacacga cgtcgggcat
       61 gccgtgaagc tagtgcagcc tggtgtgttc gtcgctcggc gaattgctgc cggaccgatg
      121 gcgtgtgaga tggcaccatt cgttccccat ggggcgcctt cgtgccgtat gtggcatcaa
      181 agtcgaccca tgggggtcgg atggtgccgt gtgtcgtggc ggtcgtcgcc gcggcccggt
      241 ctgctgccga atagcttggg tgcatgcgac tcacgccgac ggaccgcgac cggctcctca
      301 tcttcaccgc cgcggagctg gcgcgttgca tccccgggcc cgaagcgcag ttgcgcgtca
      361 tttaccatgg cgtctctgag agctggttcc atgtggagaa gggcgcgcag ccccacgaac
      421 gcccctacct gctctacgtg ggcaacgtca agccgcataa gaacctcaga cgcctgctga
      481 acgccttcga gttggtgcga gaccggatcc cgcacgacct gctgatcgtg ggccagcgcg
      541 agggattcat cacccccgat gaggcggttg gagccaaggc gttggagctg gggaaccgcg
      601 tgcactttac gggtcatgtg gataacgcca cgctgcagca gtgctatgcc cacgcggatg
      661 cgctggtctt cccgtcgctg tatgagggct tcggattgcc gcccctggag gcgatggcct
      721 gcggctgccc cgctgccgtc tcgcgtgccg cgtcgctccc cgaggtatgc ggtgacgcag
      781 cgctctactt cgatccgcat aacgagtcgg atatcgccga gaagatcgag cggatcgtga
      841 gcgatgtgga actgcggtca gagctggtgg cgcggggaaa agagcgcgcg cactccttcc
      901 gctggcagga caccgcggca tcggtatgga acgtgatagg gtggcgggcg tagcctgagc
      961 acccgttcaa tgggcccccc ggttggcaac ggtccatcca caggggctga atgccgcgag
     1021 tgttcgccga tggaagaagt aaccgcaagc cagctgatcc gcattgacga agccgaggcg
     1081 cagatcgccg aggcggcagc cgcagcgcgc ccacggtgca cagcggtgat actggcactg
     1141 gcggatctca tctcgctgct tgctgcatgg ggcatcagtg ttagccttgc ccacttgctc
     1201 cacacggggc ccactccgga cttctactgg gcgctcctgc ccgcagccgt gatcttcctc
     1261 gccgtgtacg cggcattcgg actctatccc accgtcggtc acgggccggc cgaggagcta
     1321 aagcgcacct ctctggcaac cacgtgggct tatacgatct ccgccacggc tgccttcctg
     1381 acgcgcacgg aagcgccatg cccggcagga gtgatcatcg cggcctgggt gctctcgctt
     1441 gccctggtgc cgatctgccg ttcagtggcg cgggcgctct acgcgcgcca gcggtggtgg
     1501 ggtcatccgg tggtgatctt cggcgcagga cgcacgggtg ccctgatcat ccgcgcgcta
     1561 cgagactgtc ctgacttggg tctcaaccca atcgccgtcg tcgatgatga tcccgcgcgc
     1621 tggggccgcc tggacggaac ccccgtggtt ggcccgctgg agaaggcggt cacactggca
     1681 aaggcgcact cgatacgcta cgccatcgtc gcgatgcctg gcgtagcgcg cgaacgcctg
     1741 ttgagtatca tcgagcagta cgggaagccg ttccgccacg ttctcgtggt gcccgatctc
     1801 tacgggattg ccagcctttg ggtggtgggc cgcgacctgg gaggaatgct cacgctcgaa
     1861 gttcgccggc ggcgcctgat gccgatccac aaggcgatca agcgggcgct ggatatcgtt
     1921 ctggtgcttc tctccagcgt cgtcgtcttg ccgatcttcg ccctcatcgc cctggcagtt
     1981 cgcctcgatt ccccggggcc cattctctac ggaagcattc gcatcggccg gaatggaagg
     2041 cgcttcaccg cctggaagtt tcgatcgatg gtcaccaacg ccgatgaggt gattcaggag
     2101 tatctgaggc agaaccccaa ggagcgagag gaatatgagc gcgttcacaa gctccgccag
     2161 gatccccgcg tcacccgagt cggccggttt cttcgacgaa ccagcctgga tgagctgccg
     2221 caactgtgga acgtgctgct tggcgatatg agcctggtcg ggccgcggcc ctacgccgag
     2281 tatgacttcc gcgacgacaa cctggaggag tttgacctct attaccaggt cctgccgggc
     2341 ttgaccgggc tctggcaggt gtcggggcgg gcggagacga ccttcgccga acgcatcgcc
     2401 ttcgacaatt attacgtgcg caactggtct ctctggctcg atctctacat ccttgcccga
     2461 acggtttggg ttgtcctctt tggacgcggc gcctactgag ccgggcgtcg tgagtgctga
     2521 tggagtgttg gaaggaaggc tgcgaccatg cgtgtcgcta ttgtccacga ctggctcacc
     2581 acctacgcag gcgctgagaa gaccctcgag cagatgctcg ccgtcttccc atcggctacc
     2641 ctctacagct tgctggattt tatgcccccg gatcagcgcc gtttcctgcg cgaccggccg
     2701 gtgaagacga cgttcatcca gcgcatgccg ctggcgcggc gcaaccaccg cgtctacatc
     2761 ccgttgatgc ccctggcgat cgaatcgctg gatctctcgg gatatgatgt agtcctctcc
     2821 agttcctacg ccgtcgccaa gggcgtactg acgggtcctg accaactcca cgtgagctac
     2881 tgctactcgc cgatgcgcta cgcgtgggac cttcagcacc agtacctggg tgaatcgggc
     2941 ctgcgctctg gcgtccgctc gctgctcgcg cgcatcctcc tccactacat ccggatgtgg
     3001 gacgtgcgca ctgccgccgg tgtcgaccat ttcgtggcga tttccgactt catcgcgcgg
     3061 cgaatacgga aggtgtaccg gcgcgaggca gccgtgatct accctccagt ggacgtttcc
     3121 gctcgcaagg tgcgcacgga gaaggaggat ttctacgtca cggtatcgcg ccttgtgccc
     3181 tacaagaagg tgccgctgat catagaggca ttttcccgca tgccggagaa aaaactggtg
     3241 gttatcggcg atggccccga gttggggaaa gcgcgggcgt gcgccggccc gaacgtgcag
     3301 gtcctcggcc ggcagccgta tgaggcggtg aacgacgcaa tcgctaaggc acgcgccttc
     3361 gtctttgccg cggaggagga cttcggtatc gcgccggtgg aggcgcaagc ctgcggcacc
     3421 ccggtgatcg cctacggcaa gggcgggtct accgagacag tggtgcctgg gaagaccggg
     3481 ctcttcttct cggagcagac ggttgaagcg atctgcgacg ccgtacagcg gtttgaggcg
     3541 tgccggtcgg attttgatcc ggcgcagatc cgcgcgaacg cggagcgctt tagcgacgac
     3601 cggttccggg aggagctcgg caggttcgtc cattccgcct gggcggcttt ccaggccgga
     3661 agccgtggct ccgcggtgca cgaagactcg ctcgtgccta cagggcgtga tgtcatggcc
     3721 tgacgcttgc cgtccgcttc accccccgca ggacgattgg gcacccggca caagggtgca
     3781 gtgataggat gcaggagtcg ttgtgaagat actgatcacg ggaggcgcgg gcttcatcgg
     3841 ttgcaacgcc gcccggcact tcatgcagcg cgggcatgag gtcgtcgttt tcgacaacct
     3901 gtcgcgcaaa ggcgcgagcg ccaacctgga gtggctgcgc tcccaaggcc gattcgcttt
     3961 catcgcgggc gacgtgcgcg atagccgcca ggtaatggac gcggttggag gctgcgacgt
     4021 cgtcctgcac cttgccgcgc aggtggcggt cacacggtcg attgtagacc cgcgcgagga
     4081 cttcgagaca aacgcgctcg gcacgctgaa cgtgctggaa gccgcacggc tcgccacgca
     4141 tccgcccatc gtcctcttct cctctaccaa caaagtgtat ggcgatatgg cgaacacccc
     4201 catcgtggag gagagcgacc attaccggct ggccgacctg cctttcggcg tctctgagga
     4261 gtatcccctc aacttcctct cgccctacgg gtgctctaag ggagctgcag accagtatac
     4321 gagggactat gcgcgcatct acggcctgcg aactgtggtg ctgcgccagt cgtgtatcta
     4381 tggcacccgc cagctggggg tggaggatca gggctgggtg gcgtggttcc tggccgccac
     4441 gctccttgac cggccgatca ccctctatgg cgatgggaag caggtgcgcg atctcctttt
     4501 cgtggatgac ctggtggagc tgtacgagcg cgtgatcgag cggatcgact gcgtttcggg
     4561 cgaggtgttc aacgtggggg gcgggccggc caaccagctc tcgctgctcc agtgcctgga
     4621 acagatccga aaggtgacgg gcaaggaacc cgccatctcc ttcgcgccgt ggcgtccggg
     4681 cgaccaacgc gtcttcgtcg ccgatgtgcg gagggcatca gacatggtcg gctggcagcc
     4741 gaaaaccggc gtggcggccg gcatcgccgc gctctacgag tgggcaagcc agaatcgcga
     4801 cctgttggcc agcgttctat cggagccctc accgggaaga cccggcagca tgggaaggaa
     4861 ttgaggaatg acaggcaagc tgagagccgc catcctggcg gcgagcatca tccagggcac
     4921 ggcggcgtgc gcggcgccgg cggcttccca agcgaccgcc ccgccgccca tcagcctctc
     4981 gggatcctcc gggctggtcc gcatcccatc cgcggatgtg ctcccctacg gggactacag
     5041 cttgggcgtg aacttcctgg aggcgaagta ccgttcgtac tgggactccg acaccatgct
     5101 ccacttcgca tccgtcgccg tcttcccgca ggtggaggtc accgcccgga tcaccaatat
     5161 tgacggccgg ttgggcgttc agtattacga cctgttaccc ggccagccct tcggaggatg
     5221 gaacttcgac cgggcgtttg gcgtccacgt gcagctcctg aagcaatcag cgcgcgtgcc
     5281 cgtatcgctg gccgtcggtg atcaggatgt gacaggcaac acggcgttcg gggcacgtta
     5341 cttcgtcgcc agccaccggc agccgcgata tggcatccat ctcggcaccg gcaatgggtt
     5401 cttgggaaag ttgttcgcgg gggttgatta ccagcctcat cctaaggtgc ggatcgccat
     5461 tgaccacgat ggcaaccggg ccaacctcgg gtgcacgatt cagatccagc gtctcctgct
     5521 gatgccgacg ctatcaggct tccgctcgct cggtggaggg attgcgtact ggcaacggat
     5581 gtaaggaacg atcagccact cggaggtcgc ggcatggggg cacgtccacc aggcctgctc
     5641 gacgcgcgca atcgcgcggt aaagcgagta atcgactacc tgctcgggct tcccgcgttc
     5701 ctcctgagtc tgcctgtcat cgcattcttc gccctctgga tcaaagccac cggccatggc
     5761 tctgccttct attcgcagga gcgcgaaggc atcgacggca ctcgaatccg cgtctggaaa
     5821 ctgcggacga tgtatcccga cgcggaggca acactgcgcc gctatctgga cgagaatccg
     5881 gaggcagatg ccgaatggca gcgctactgc aagctgagga acgatccgcg cgtgcttccg
     5941 gggctcggct ctttcctccg gcgaactagc ctcgatgagc taccccagct ctggaacgtc
     6001 ctccgaggcg atatgagcct ggtgggcccc cgaccctacc ccgactacca cctggatcgc
     6061 ttccatcccg agtttcgcgc gcttcggcgt agcgtgctgc ccggaatcac cgggctctgg
     6121 caggtgacta cgcggagcga gggcggcctg gatgtccagc aggcgctcga cacgcgctac
     6181 atcctcggct ggtcgatctg gttggatctc gtgattctcc tgcgcacgat cccgtgcgtg
     6241 ctcagccggc gaggcgcctg gtaggcgctg ccccaaggcg gcggcacgag agcatggagc
     6301 cccacgctgg gtgtgccgct cgggcgcgga cgtctgtgcc gcgccacagt tgctacgggc
     6361 gagcgttccg tgttcgccct ggttccggga cggggcgaac acggaatctt ccccctgccg
     6421 gtgccatggc cgcgttattc ttcagagacc atcacccccg cgcctctggg aacccacatc
     6481 tccccatccg tgtctgtccc catcatccgt ggtatcaccg acggcgccgg gcggcgggta
     6541 gggaatgggg ccgggcgcag gcgtccggct tcgggcccca gaccatgtcc ctctcgcgcg
     6601 ccttgacttg gcatcgccgc ggagcgtata atacccacaa caaacgagtt tgcacgccac
     6661 catcaggggc caaggggttt cagcacttgc tagagaggct gcgcgtgggc ggacgctact
     6721 ggttacctcc gctcttatgg acggtcctgg ttcttgcctt ttccaccgcg tccttctctg
     6781 gcgagcagac cgggggaatc ctggagcgcg tgctgggcct cctatggccg gccgggctcg
     6841 acccatggca gctggagtgg gttcacctcg gcattcgcaa gctggcccac tttctggagt
     6901 atgccggcct cgcgtttctg tggtcactcg ggttttcccg cgccggcggc tatccggggc
     6961 aggctgcttt ccagatggcg ttgcttgcaa gcgccttggc ggcggtgatc gacgaggcgc
     7021 atcaggcggt gtgtccggag cgcaccggcg ccctggcaga tgtgctattg gattgcatgg
     7081 gcgcggtgag cggcggggct atctatcgcg tttgggaaag gaggcacgcc aagtgcctaa
     7141 catcggattc cctgaactcg tcgtcctcct ggtaatcgtc ctcctgctct tcggtccgaa
     7201 gaagctcccg gatttcgcga aatccatggg gcaggcgatt cgcgagttcc gcagggcatc
     7261 tcaggaggta gtggacgata ttcggaaggc ggctgacgag acgccagcgg cctccgaaag
     7321 tggccggaac gcggcttacg cccagacagc ggcatccgag gtcgagcgtc ccgcggcgga
     7381 gccgagccag aagcagctat gaacaggccg ctggagatcc gcccgctgac gctgctggcc
     7441 ctcctcgccg cgttctatct gggcggcctc tttgggccgg ctcgccggca aacagaagac
     7501 cgggtgcccc ctgccccggc acccctctcc acggcacagg cggttgccgc gccctcggcg
     7561 gtggtccgca acgacgccat cgctgacgct gcagagaagg caggcccggc cgtggtgcgc
     7621 atcgataccc tgaccgtgca gcgggatccc tgggaggagc tgttcggtcc cgacccgttc
     7681 tttggcccac ggggtcgtgt gagcagcggc acgggctctg gggtcctcat ctcgcccgag
     7741 ggctacatcc tgaccaacga gcatgttatc tccggggcga gccagatcac cgtgtggctg
     7801 gcggacgggc gccagctcaa gggagaagtg gtcggtggcg attcgccctc ggatctggct
     7861 gttgtgaagg ttccgcccaa ggacctaccg gatcctccac gtatctccac ggcgccgctt
     7921 cgccctggcc aatgggccat cgccatcggg aatccttttg gcctggaaca caccgtgacc
     7981 ttgggggtga tcagtgccac gaagcgcccg atgcagattg aggatcgcac ctacgacaac
     8041 ctgatccaga ccgactgcgc catcaacccc ggaaactctg gcgggccgtt gattgatatc
     8101 aacggcgatg tgatcgggat caacacggcg attctggcca atgggcaagg ccttggcttc
     8161 gccaacccaa tcggctttgc caagagcgtc gccgatgagc tgatccggta tggcaaggtg
     8221 aaacgcccct ggcccggcct ctatgtgcgc acgatcacgc ccggcttggc ggcgcgcctt
     8281 ggccggcgcg acacgcgcgg tgcgctcgtc atcggcatca ttcgcaacag tccggcgcac
     8341 gtcgctggcc tccgccgtgg cgatatcatc ctgaaagtgg atggccgcga cgtcaccaac
     8401 ggcgacgagc tgatgctgga cctgaagaag cgccggatcg gcgaccaggt gaagctgctc
     8461 atcgagcgcg atggctacac gggcacgacg gagatggaga tcggcgaggc accctgacgc
     8521 gcggctcgat cgggcgtgac gcctcgcccc gtacgcaatc aggccccgcg ccatccgagg
     8581 aaggcgcggg gcctgactct ttttacggca agaataccgt tacttatcgg cgatgcgcgc
     8641 ggcggcagcc gtatcgatcc gcagccccgg tcccatggtg gacgagatca cgatctgctg
     8701 caggtagtgc cccttggcag cagcgggtcg cgccttgaga atggcgccaa tcgctgcgga
     8761 gaagttctcg cggagctgat cgacggtgta gctcgccttg ccaatcggca tgtggacgat
     8821 acccgtcttc tccaccttga actcgacacg cccgcccgtc ttcacctcac gcacggcgcg
     8881 cgcgatatcc ggggtgacgg tgcccgcctt cggatttggc atgcgctgcg tgagcacgcg
     8941 gcccagacgc cccacgatgc ccatcacatc cggggtggcc aggaagacat cgaactcctt
     9001 gtccgcgccg gcctggatct tggccaccag gtcctcggca ccaacggcat cggcgccggc
     9061 ttcctccgcg gcctgcgcgt tctcgccccg cgcaaagacg gccacgcggc gcttcttgcc
     9121 cgagccatgc ggcagcgggg taacgccacg caccatctgg tcgccatgcc gggggtccac
     9181 gcccagcttc accgccagat cgacggtctc atcgaacttg gcattggcaa tcttcttgac
     9241 gatttcgatc gcctcgaccg gatcgtaaat acggctctta tcgacctggg ccgccgactc
     9301 ctggtagcgc tgcagcgccg accacgagcg gccgagcagg agcc
//
LOCUS       DAOSCV010000034         9330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4842_length_9330_cov_3.366253, whole genome shotgun sequence.
ACCESSION   DAOSCV010000034 DAOSCV010000000
VERSION     DAOSCV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4842_length_9330_cov_3.366253"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1558
                     /locus_tag="PLY56_01660"
     CDS             176..1558
                     /locus_tag="PLY56_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20215.1"
                     /translation="MITPTVGFIVYGVHKDGLKDPMGTPFIDDALIASSKKALKEAGI
                     NLVEWPVIIATKEEAKAALAAMKKDDRVDCVVLFSGTWVWAAHLIAAVRDFAMTGKGV
                     LVWTHPGSQGWRPVGGLVMHGALMEVGIPHKFVYGDANDPAEIAKLASFARASHMKQW
                     LNMSTIGTFGGRGMGQTCGVADPSQWMRMFGCDIDSRDTTELIRTANAITSEQITALE
                     PRIQELFGNVPERNVVNERSIRLYLAIKEIVEKEKWDFYTIQSFPGLGDDYTASCFAQ
                     SMMLEDGVGTSTLGDFNTALTVLLLTKLSKEPVYYGDLQHIDKRNNEIKIIGDGACPP
                     SLAGKLGPAGFAEHGIPTEGEAGGLSVKLVCKVGEGVLARLGRVDGEFVMAITRCSIF
                     EPPADQLEKRQLECGIPFWPHGFVTAHCDIEALLQSWTNEYACLGYGTHLYDDLVEFC
                     EMTGITPIAL"
     gene            1686..2819
                     /locus_tag="PLY56_01665"
     CDS             1686..2819
                     /locus_tag="PLY56_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20216.1"
                     /translation="MIGFGNRIGSDFDNVSQRLVYNYIATQPDFRPVPSKIASEHAQR
                     QFYDFIAGVFTALYHNPERIGMTMDPDETEDRPRPDRVYEDESRKYLAARKLRRQNEE
                     KIEGLFDGMQRLAELGTVGRGALVVRRGELGIQRRALETLVENLTRLGLRCSMDDQAL
                     CLCSDAYPEMWDAWKLLATVGAAEAAAYDARWRQEVGDEARLPKWQVTTHRRYLSQIK
                     NRIFKRALFDPAYDFSVDVLCRLCDDPAGYRQFADELLAQGFVKRAGWDGGLDVTNFI
                     DLVFTKTYVGPGGEPLKLQFRTRFESRYYRQILYTFVEDKVAVKTLLREFGALPETLQ
                     DVVYGFTGECRACGFCNQTNRKLQYNNVPAVYRGRPVRLCYYF"
     gene            3008..4075
                     /locus_tag="PLY56_01670"
     CDS             3008..4075
                     /locus_tag="PLY56_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOJ20217.1"
                     /translation="MRIGNRHYTRAEVLERVGSIRQLGGVRRCILDDGRSKGVTAFEV
                     DTGAGFRFTVLPDRGLDISSASFRGTNLVHLTQNGEAHPAFYEPQGLGWLRTFFAGLL
                     TTCGLTYLGPPGQDGEEILGLHGRATTTPARQVCDLSRWEGDEYVLEVTGIIEESHLF
                     GDKIRLTRTISTRMGSRSLTIRDVAENFGYRTAPFHILYHVNAGFPLLDACSYLVLSP
                     CESEACDAHSAAGMEQMRIFTAPIPGFEEENFLHRMYPGPDGWATAAMVNPELEGGLA
                     LYVRFDARALPLLNEWKMMGQGDYVVGVEPCNAPCENRAILREQGRLPFLEPGETREL
                     NLEIGVLQGAEEIERLVKQAE"
     gene            4115..5227
                     /locus_tag="PLY56_01675"
     CDS             4115..5227
                     /locus_tag="PLY56_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20218.1"
                     /translation="MARVKPDLLLKDYKPVRLLKRPDNTPERAKFPVIDAHNHLFGDP
                     SPEEMLAVMDAVGVRTFVNVTGNVGMPLVDNCYTIERREFDSFLRDYVEPHPGRFAAL
                     TMSEFARWGDFTLPLRPGPGRQEFVDRAIEGLEADVAKGALGLKVTKELGLQFRDESG
                     AMVAVDDERLFPIWRRAGELGLPVLIHVSDPRGFFLPIDERNEHYLNLLEFPGWSFYG
                     SHFSKDELLEQRNRVIAAHPKTTFFLPHVANDPEDLDSIAALLDRYPNVVIDFSARID
                     ELGRQPYSARDFLVYYQDRVLFGSDMPLSTEMYRCYFRFLETRDECFDYMDYIGRWGH
                     SRWGIYGLYLPDEVLRKIYYENAIRLWPALEAAIAE"
     gene            5306..6358
                     /locus_tag="PLY56_01680"
     CDS             5306..6358
                     /locus_tag="PLY56_01680"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ20219.1"
                     /translation="MPLVTDYHQVKEVYQEATELGVALPVFCAEDRETLEAILASALE
                     MGKAIGVEDLPIIPAWTVRYHGRPQATLITACGDPLLGVRLHLSDLDVLMSDGSPYAK
                     LRVMPHIDHGIPWLDEDVLLGLVDRVASVMCDASEKPFAENIRITAEYVERVRGRVVV
                     EGAVDEIYAADGAGGMKNEPTTVEQAVKFLRETGVDILVPNVGTEHRMTGDHVVYRSD
                     RAREISAAVGRILCIHGTTSARPEDLPKLPGDGFIKVNIYTTLAVRGGQALARHVLRN
                     LGNILPEEELRELVEAGVLGERVLAPDYGENRMPLKPRLDRVANASRRDAWFAAVRDR
                     CKEFLGIFGYQRYAGR"
     gene            complement(6432..7550)
                     /locus_tag="PLY56_01685"
     CDS             complement(6432..7550)
                     /locus_tag="PLY56_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ20220.1"
                     /translation="MIYRNLGKTGIQVSVIGFGGMRFFNKSEEEAIATVHRAFERGIN
                     FFETGSYGNGKSEEMLGRALWQVARRDQVILANKARASERPTAASVRAELEASLKRYQ
                     TDYFDIFNFWGTNTPEMHRHILEHGALEAALQAREEGLIRAIGLTTHARPEWIRDFVD
                     AYPWECIILKEHMLYSRNQEVIDYLGSKGIGVVVMTPLAGGVVATPSEEIRAEVAREG
                     LTPAQLGLRTLIANPNISSAISGMTSPSEVDENVEAARTDGPLTPAEQRLVDWIRQKT
                     QALGERFCTGCSYCMPCPQEVNIPGIFRLWNLMRGYGNAEYSKLEYQKIREQRHWADF
                     PGRSVEHCVECGTCEEKCPERLSIIDDLKRAHAELTAQ"
     gene            complement(7752..8327)
                     /locus_tag="PLY56_01690"
     CDS             complement(7752..8327)
                     /locus_tag="PLY56_01690"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOJ20221.1"
                     /translation="MQSRPVVATEQPSEQAAWAERNTEPSRWYALYTRSNYEARVASV
                     LSAQSVETYVPTCPAWSRRSRVPRLADTPIFPGYVFIHSPLTKEVWLTVKKTTGVVRI
                     VGCGNEPTPIPDHEIESLRTVLAVQPVVVGHPRLRAGQHVRVVRGPLAGVYGTLVHVA
                     RNRHRLVITVELLNRSVSTSIDASLVEPCDT"
     gene            9020..>9330
                     /locus_tag="PLY56_01695"
     CDS             9020..>9330
                     /locus_tag="PLY56_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20222.1"
                     /translation="MRGTGEMMGVCMPKAQAVEPQKLLETTGASAEEIQADAEAMRIV
                     EAYRRARDEYHEARAHLTVVEANGGRLAVWRARNRCRAALRHFSSMGEQLTAMTQTHP
                     A"
BASE COUNT         1627 a         2854 c         3138 g         1711 t
ORIGIN      
        1 gcggggttgc tgccgcgccg tgatgccggc gtggtttact ttcccatact tcggaagccc
       61 gggagcgctc tggtctccgc cggcagcaag gagtagtggc tgtttccgcc ccgatctatc
      121 caggtgacgg agccctgggg aacaccgggc ttcctggagt agcaaggagt agctcatgat
      181 caccccgaca gttggtttca tcgtctatgg cgttcataag gatggcctga aggacccgat
      241 gggcacgccc ttcatcgatg acgcgctgat cgcgagttcg aagaaggcgc tgaaagaggc
      301 tgggatcaat ctggtcgagt ggccggtgat catcgccacg aaggaagagg cgaaggccgc
      361 cctggccgcg atgaagaagg atgaccgcgt cgactgcgtg gtcctcttct cgggcacgtg
      421 ggtctgggcc gcgcacctga tcgccgcggt gcgcgacttc gccatgaccg ggaagggagt
      481 gctcgtctgg acgcatcccg gctcgcaggg ctggcgcccc gtgggcggcc tggtgatgca
      541 cggcgcattg atggaagtgg gcatccctca caagttcgtt tacggcgatg ccaacgaccc
      601 ggccgagatc gccaagctcg cgagcttcgc gcgcgcctcg cacatgaagc agtggctcaa
      661 tatgtccacc atcggcacct tcggtggccg gggcatgggc cagacgtgtg gcgttgccga
      721 tccctcgcag tggatgcgca tgttcggttg cgacatcgac tcgcgcgaca ccacggagct
      781 gatccgcacc gcgaacgcga tcacctcgga gcagatcacg gccctcgagc cgcggatcca
      841 ggagctcttc gggaacgtgc cggagcgcaa cgtcgtcaac gagcgctcga tccgcctcta
      901 cctggccatc aaggagatcg tcgagaaaga gaagtgggat ttctacacca ttcagtcttt
      961 ccccggcctg ggcgatgact acaccgcgtc ttgcttcgcg cagagcatga tgctcgagga
     1021 tggcgttggc acctcgacgc tgggcgactt caacaccgcg ctcaccgttc tgctcctcac
     1081 caagttgagc aaggagccgg tctattacgg tgacctgcag catatcgata agcggaacaa
     1141 cgagatcaag atcatcggtg atggcgcctg cccgccgtcg ctcgcgggca agctgggccc
     1201 ggccggcttc gcggagcatg gcattcccac cgagggtgag gcgggcggtc tctcggtgaa
     1261 gctcgtctgc aaagtgggcg agggcgttct ggcccggctc gggcgcgtgg acggcgagtt
     1321 cgtgatggcg atcacccgct gctccatctt cgagccaccg gcggatcagc tggagaagcg
     1381 ccagctggag tgcgggattc cgttctggcc tcatggtttc gttacggccc actgcgacat
     1441 cgaggcgctc ctgcagagct ggacgaatga gtacgcctgc ctcggctatg gcacccacct
     1501 gtatgacgac ctggtggagt tctgcgagat gaccggcatc acgccgatcg cgctctgagg
     1561 ctggctttcg tcgccacttc cgtggcgttt ggttccgcgc cggccccgaa gggccggcgc
     1621 ggctgcccct ttccgtcggg ataggcagga cgcctcgctt cgcggggcag aaggggaacg
     1681 agccgatgat cgggttcggg aaccgtatcg gtagcgactt cgacaacgtg tcgcagcgcc
     1741 tcgtctacaa ctacatcgct acccagcctg atttccggcc cgttccctcg aagatcgcga
     1801 gcgagcacgc gcagcgccag ttctacgact tcatcgccgg cgtcttcacc gcgctctatc
     1861 acaacccgga gcggatcggg atgacgatgg atccggatga gacggaggac cgcccccgac
     1921 ccgatagggt ctacgaggac gagagccgca agtacctggc ggcgcgcaag ctccggcgcc
     1981 agaacgagga gaagatcgag ggcctgttcg atggcatgca gcgcctggcc gagctgggaa
     2041 cggtgggccg cggggcgctg gtggtccggc gcggcgagct cgggattcaa aggcgcgctc
     2101 tcgagacgct ggtggagaac ctgacccgcc tgggcctgcg ttgctcgatg gacgaccagg
     2161 ccctttgcct gtgcagcgac gcctatcccg agatgtggga tgcctggaag ctgctcgcca
     2221 ccgtgggcgc ggcggaggct gccgcttatg atgcccggtg gcgccaggaa gtgggcgacg
     2281 aagcccggct cccgaagtgg caggtcacca cgcaccggcg ctacctcagc cagatcaaga
     2341 accgcatctt caagcgcgcg ctgttcgacc cggcgtacga cttctcggtg gatgtgctct
     2401 gccgcctctg cgacgatccg gcgggctatc ggcagtttgc ggacgagctc cttgctcagg
     2461 ggttcgtgaa gcgcgccggc tgggatggcg gcctggacgt gacgaacttc atcgacctgg
     2521 tcttcaccaa gacctacgtc gggccagggg gcgagccgct caagctccag ttccgcaccc
     2581 ggttcgagtc gcgctactat cgccaaatcc tctacacctt tgtcgaggac aaggttgcgg
     2641 tcaagacgct gttgcgagag tttggagctc ttccggaaac gttgcaggat gtggtttacg
     2701 gcttcaccgg cgaatgccgg gcgtgtgggt tctgcaacca gacaaaccgg aagctccaat
     2761 acaacaacgt gccagcggtc taccgtgggc ggccagtccg gctgtgctat tacttctaag
     2821 cggctcggca cgcgcgagct aacgcgcgag cggttggacg aggcccgggc gctctacgcc
     2881 tggatggatg ggcgttacga ggctgcgccc cccgcggcgg cggcataggc ggaggagcgc
     2941 ctggcgccaa gttcgcgcgg cgaggtccgg gagcagcttc ggatatctgt gttgataggg
     3001 agtgcgcatg agaatcggca atcggcacta cacgcgcgcc gaggtgctgg agcgcgtggg
     3061 cagcatccgg cagctggggg gcgtgcgccg gtgcatcctg gatgacggcc gctcgaaggg
     3121 cgtgaccgcg tttgaggtgg atacgggcgc cggcttccgt ttcaccgtct tgcccgaccg
     3181 gggcctcgat atctcatcgg cctccttccg gggcacgaac ctggtccacc tgacgcagaa
     3241 cggcgaggcg caccccgcgt tctacgagcc acaaggcttg ggatggcttc gtaccttctt
     3301 cgccggcctg cttaccacct gcgggcttac ctacctgggc ccccccgggc aggatggtga
     3361 ggagatcctc gggctgcatg gccgggctac aacgacgcct gcccgccagg tgtgcgacct
     3421 ctcgcgctgg gagggcgacg aatacgtgct ggaggtgacc ggcatcatcg aggagagtca
     3481 cctcttcggt gataagatcc gactgacgcg cacgatctcc acccggatgg gcagccggtc
     3541 gctcacgata cgcgacgtgg cggagaactt cgggtatcgc actgcgccgt tccacatcct
     3601 ctaccacgtg aacgccggtt tcccgcttct ggatgcctgt agctatctcg tgctcagtcc
     3661 gtgcgagagc gaggcgtgcg acgcgcactc cgctgccggc atggagcaga tgcgcatctt
     3721 caccgcgccc atccctgggt tcgaggaaga gaacttcctc caccggatgt acccggggcc
     3781 cgatggctgg gccactgcgg cgatggtcaa cccggagctc gaaggcggcc tggcgcttta
     3841 cgtgcgcttt gatgcgcgcg cgctcccgct gctcaacgag tggaagatga tggggcaggg
     3901 cgactacgtg gttggcgtcg agccgtgcaa cgccccgtgc gaaaaccgcg cgatcctgcg
     3961 cgagcaggga cgccttccgt tcctggagcc gggtgagacg cgcgagctga acctggagat
     4021 aggcgtcctg cagggcgccg aggagattga gcgacttgtg aagcaggcgg agtgaagcga
     4081 gagccgtttc ccgcctgacg catcctggag agccatggca cgcgtgaaac ctgacctgtt
     4141 gcttaaagat tacaagccgg tgcgcctgct gaagcgcccc gacaacaccc cggagcgcgc
     4201 gaagttcccg gtgatcgacg cgcacaacca cctcttcggt gacccgtcgc cggaggagat
     4261 gctggcggtc atggatgccg tcggcgtgcg cacgttcgtg aacgtcaccg gcaacgttgg
     4321 catgccgttg gttgacaact gctacaccat cgagcgccgc gagttcgact cgttcctgcg
     4381 cgactacgtg gagccgcatc cggggcggtt tgccgcgctc acgatgtcgg agttcgccag
     4441 gtggggtgac ttcacgcttc cgcttcggcc cggaccagga agacaggagt tcgtcgaccg
     4501 tgcgatcgag ggtctggaag ccgatgtggc gaagggtgcc ctggggctga aggtgacgaa
     4561 ggagctgggc cttcagttcc gcgacgagag tggtgccatg gtcgcggtgg atgacgagcg
     4621 cctcttcccg atctggcgac gcgccggcga gttaggcctg ccggtgctta tccacgtctc
     4681 ggatcctcgg ggcttcttcc tcccgatcga cgagcgcaac gagcactatc tcaatctgct
     4741 cgagttcccg gggtggagct tctacggctc tcacttctcg aaagatgagc tcctggagca
     4801 gcgcaaccgc gtgatcgcgg cgcacccgaa gacgacgttc ttcctcccgc atgtggccaa
     4861 cgacccagag gatctggatt ccatcgcggc gcttctcgac cgctacccga acgtcgtcat
     4921 cgatttctcg gcgcgtatcg acgagctcgg ccggcagccg tacagcgcgc gcgactttct
     4981 cgtctactac caggatcgcg tcctcttcgg cagcgacatg ccgctctcca ccgagatgta
     5041 tcggtgctac ttccgcttcc tggagacgcg cgacgagtgc tttgattaca tggactacat
     5101 cggccgctgg ggccatagcc gctggggcat ctatggcctc tacctgccgg acgaggtcct
     5161 ccgcaagatc tattatgaga acgccatccg cctctggccg gcgctggagg ccgcgatagc
     5221 ggaatgacga gagggctggg ggatgggaac gcccgggagg gcgtttgcct ccagtgcccc
     5281 gccatccaga ggaagggagc caggggtgcc gctagtcact gattaccatc aggtgaaaga
     5341 ggtctaccag gaagccaccg agttgggggt ggcgctgccg gtcttctgtg ccgaagaccg
     5401 cgagacgctg gaggcgatcc tcgcgtcggc gctggagatg gggaaggcga ttggcgtcga
     5461 ggacctcccg atcatcccgg cgtggaccgt gcgctatcac ggacggcccc aggctacgct
     5521 catcaccgca tgcggcgatc cgctcttggg tgtgcgcctc cacctctcgg atctcgacgt
     5581 gctcatgagc gacgggagtc cctatgccaa gctgcgcgtg atgccgcaca ttgatcatgg
     5641 gattccctgg ctggatgagg atgtgctgct cgggctggtg gatcgagttg cctcggtgat
     5701 gtgtgacgcg agcgagaaac cgttcgcgga gaatatccgg atcacggcgg agtacgtgga
     5761 gcgggttcgc ggcagggtcg tcgttgaggg cgcagtggat gagatctatg ctgccgacgg
     5821 tgccggcggg atgaagaatg agccgacgac cgtcgagcag gcggtgaagt tcctgcgcga
     5881 gaccggcgtg gatatcctgg tacccaacgt gggcacggag caccgcatga ccggcgatca
     5941 cgtcgtctac cgatccgacc gggcgcgcga gatcagcgcc gccgtcgggc gcatcctctg
     6001 catccatggc accacttcgg cgcggccaga ggatctcccg aagctgcccg gcgacggctt
     6061 catcaaggtg aacatctaca cgacgctcgc cgttcgcgga gggcaggcgc tcgcccggca
     6121 cgtgctgcgg aacctgggca acattctgcc cgaggaggag ctgcgcgagc tggtcgaggc
     6181 cggcgtcctc ggcgagcggg tgctggcgcc cgattacggc gagaaccgga tgccgctcaa
     6241 gccacgcctg gatcgcgtgg cgaacgcatc ccgccgcgat gcctggtttg ccgccgtgcg
     6301 cgaccggtgc aaggagtttc tggggatctt cggctaccag cggtatgccg ggcggtgatt
     6361 actcgccccg gggaaccaag agaagccggg cacgtctgag acgcgcccgg cttgctcaat
     6421 ctgccaacgg actattgcgc cgtcaactca gcatgcgcgc gcttcaggtc gtcgatgatt
     6481 gagaggcgct ccgggcattt ctcctcgcag gtgccgcatt ccacacagtg ctccacggag
     6541 cggccgggga aatcagccca gtggcgctgt tcccggattt tctgatattc cagcttgctg
     6601 tattcagcgt tgccgtagcc gcgcattagg ttccagagcc ggaagatgcc ggggatgttc
     6661 acctcttgag gacacggcat gcaatagctg cacccggtgc agaagcgctc gcccagagcc
     6721 tgggttttct gccggatcca gtcgacgagg cgctgttcag ccggggtgag cgggccgtca
     6781 gttcgggcgg cttcgacgtt ctcatccacc tcgctggggg aggtcatccc gctgatcgcg
     6841 ctggagatgt tggggttggc gatcagcgtg cgcaggccga gttgagcggg agtcaggccc
     6901 tcgcgggcga cttcggcgcg gatctcctcg ctgggcgtcg cgactacgcc gcctgcgagg
     6961 ggggtcatga ccaccactcc gatccccttg ctccccaggt aatcgatcac ctcctggttg
     7021 cggctgtaga gcatgtgctc cttgaggatg atgcactccc aggggtaggc atccacaaag
     7081 tcacggatcc actccgggcg tgcgtgcgtg gtgagcccga tggcgcggat caacccctcc
     7141 tcacgggcct ggagcgccgc ttcgagtgcg ccgtgctcca ggatgtgccg gtgcatctcc
     7201 ggcgtgttgg tgccccaaaa gttgaagata tcgaagtagt ccgtctgata ccgcttgagc
     7261 gatgcctcta gctccgcgcg gaccgaggcg gcggtgggcc tctccgaagc acgggcctta
     7321 ttggcaagga tcacctggtc gcgccgcgcc acctgccata gcgcccggcc cagcatctcc
     7381 tcactcttgc cgttgccgta gctccccgtt tcgaagaagt taatccccct ctcaaaggcg
     7441 cggtgaaccg tcgcaatcgc ttcctcctcg ctcttgttga agaagcgcat cccgccgaag
     7501 ccgatgacgg agacctggat gccagtcttg cccagattac gatagatcat acctcggtta
     7561 tccccctttc gggcgccggc ggagcacggc gcggttgtct tgaagccggg tggcaagcgc
     7621 ggtgtggatg gggatcgccg ccttccctga gccatagttc gtcgcgggct gtcggatctc
     7681 ccaggggcgc gcgctggcgc tgtcggagcc cgctacggcg agcctcgaca gcaccgatgg
     7741 cggcaggtcg attaggtatc gcaaggctcg acgagacttg catcgatgga tgtgctcacc
     7801 gagcggttga gaagctccac ggtgattact aggcgatgcc ggtttcgcgc gacgtgaacg
     7861 agggtgccat agacgccggc gagtggcccg cgtacgacac gaacatgctg accggcacgc
     7921 agccgtgggt ggcctaccac gaccggctgc accgcaagga cggttcgtag cgactcaatc
     7981 tcgtgatcgg ggataggcgt tggctcgttt ccacagccca caatgcgcac tacccccgtc
     8041 gtcttcttga cggtgagcca cacctccttc gtgagggggc tatgaatgaa gacatatccc
     8101 gggaagatgg gcgtatcggc gagacggggt actcggctgc gccggctcca ggccgggcag
     8161 gtggggacat aggtctccac cgactgtgct gatagcacgc ttgcgacacg tgcctcgtaa
     8221 ttactgcggg tgtagagtgc gtaccatcgg gagggctcag tattgcgttc agcccaggcc
     8281 gcttgttctg acggctgctc cgtggcaacg actggccgtg actgcattac gattacctcc
     8341 agcatctagg gtctgatgca ccgcagaatg caattcgcat agccggacag acgcacgctc
     8401 gctggtccgc atctatcgcg cctgcctaca ctcagcagcc actactctcc ccgtgcttgc
     8461 gggctaccgg ttggataggc atgtagcagc actcggcagt gcgatagtag atgcgcgaaa
     8521 caggcaccac aggtggcggc cagcagagta gctcccgact ccagtctggt tgcgacaccg
     8581 gaatcttgcg atcgactgcc accatggggt gcgcaggaaa acccggaact gaagtgacca
     8641 tggggagaac tcgtgcgccc ttgagtgccg aaagggatgg cccctttggt ccagacactt
     8701 caccaccaag tggtggcttt tattatacac catccatcgt gttgtgtaaa ccgtaggata
     8761 agtccctatc aggaggcccg gcgttggcta gggaaggtga ggtatggcct gcgccagtgc
     8821 gccatgtcgg ggcgtaggac ggggggggga acggttatgg cacgcacgtc gtctcttact
     8881 tagagggctc accgtcctga gtgtgtgcga tggcaagagc ggcggggctg ccgagacagg
     8941 ggcttaagtg ttcgggccag agtgccgaaa gaggaagatg aggccgtagc gtggccggat
     9001 ccagacagga tcgcttccca tgagagggac aggtgagatg atgggtgtct gcatgccgaa
     9061 ggcgcaagcg gtagagccgc agaagttgtt ggagacaacc ggagcatctg cggaggagat
     9121 ccaggcggat gcggaggcga tgcgaatcgt tgaggcgtat cggcgagcac gcgatgagta
     9181 ccatgaagcg cgcgcgcatc tgacggtcgt ggaagctaac ggcggccgtc tggcagtctg
     9241 gagggcgcgc aaccgctgcc gcgcggcgct gcgccacttc tccagcatgg gagagcagct
     9301 aacggcgatg acccagactc accctgcgtt
//
LOCUS       DAOSCV010000035         9330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4843_length_9330_cov_2.982426, whole genome shotgun sequence.
ACCESSION   DAOSCV010000035 DAOSCV010000000
VERSION     DAOSCV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9330
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4843_length_9330_cov_2.982426"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLY56_01700"
     CDS             <1..384
                     /locus_tag="PLY56_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ20223.1"
                     /translation="LHAFPVKRGSGDRAALRRAEQLLSQGELVVIFPEGRESETGELI
                     AFQPGAALLSLRAGVPVIPICLKGTDGVMPYGTVIPRRSPRPTVVRIGPPVCLDDLRD
                     HPDRRQALMEGAERIRAAVAALADG"
     gene            414..776
                     /locus_tag="PLY56_01705"
     CDS             414..776
                     /locus_tag="PLY56_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOJ20224.1"
                     /translation="MEIHEMQAEVDRWISQFEEGYWHPLSQLARLTEEVGELAREINH
                     RFGEKPKKPEEAEANLALEIADVLFILVALANSQGIDLERAFREMMEKYRARDSERWT
                     PRSTPTPEPPESSEPTPQ"
     gene            complement(763..1887)
                     /locus_tag="PLY56_01710"
     CDS             complement(763..1887)
                     /locus_tag="PLY56_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20225.1"
                     /translation="MGSAYTPGLKVSPFITIQKTRRLPLKGQVLVAPGDHVEPSTPVA
                     RTELPGNAQMVRAAQMLGIEPREIWRALLKQAGDSVQKGELLAETRSFFGLFRATVTA
                     PISGTIESVSDTTGNITIREAPIPVEVPAYVEGTIVEVLPGEGVVVEARGAFIQGIFG
                     VGGEREGKLRVVVSGPDQPLDEAQVPAECAGEILIGGSLVTGAALRAAAKAGAAGIVA
                     GGIIDQELMEFLGHDIGVAITGHENIHLTLMVTEGFGEIRMAERTFELLRSLDGRRAS
                     INGATQIRAGVIRPEIIVPRTQEAQAGMTTAEDESALEIGTRIRVIREPYFGRLGRVA
                     TLPPEPQQIPTGASVRVLGADLDGGEHVVIPRANVEIIVE"
     gene            complement(2327..4990)
                     /locus_tag="PLY56_01715"
     CDS             complement(2327..4990)
                     /locus_tag="PLY56_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ20226.1"
                     /translation="MKLSAVHLIHHTHMDIGYTDLPGEVLEQHLAHLDRALVLCQGNA
                     DLPEDRQFRWTCESALLVQEYLRCRPRAHRERLLHALRAGWVELQACLTQPLTELACA
                     EELIRCIGYAAELARQEGFPLEAATIDDIGGYAGRLPTLLQGFGIRYLIAGVGGFQVH
                     LPWADLPYLFYLESKDGARVLVWNLGQDRQVRPQELTAIRAVYGLASIHLIPPYREEL
                     ARGRREGVPTARERFAQLVTRLENDGYPFDEVMLQYANDNGGPDEGLVDLIARINATG
                     ETPPIRLTTPCDFLRKMEARYGESIPVLRGVLTDPWVMRANPTPSPLKKYRQAQRTLE
                     AAEAYAAFEREPSPDPGEPARVEEVHANLQLYSDHTYGLSAWGWENSLDPEASTRAAA
                     YDRHRESWACKRVYAEAARQGAAFLERAARQRLGATVHSTEPFVLVWNPATVPLSEPV
                     ELYLWRGGPELAGLTDAETGEAVPCQRIGNRRYLLRAPTVPAFGYRRLIPRFGTLPDS
                     GSSEPRLELENDYLRLRIDSEIGAVTSIYDKRSGRERLDQASGIGFGELLYHRYEAVA
                     FGTGKAGMGREWPRVAIPERPAGWRIGLWGPVAQSVVAHGVIEGPSGAIRMTREVILY
                     AHAPRIDLRIRVDKPETPAKECCYVALPFAGGNAEFRFEQAIGWVQPAEDLLPGAMQD
                     AFYCPSWIDLASETGGITVTSPDAPVFQFGRIRTGEWLETLPFTAGSSHLYGWLYHNL
                     LDTDCALWQELLDTFTYSIHFRPGGGLDSIAARRAAAALQQPLFAEMRAPNPLGDDRR
                     PARGFIRITPESVRLLSLRRLGPGMVRLRLEETEGRAQRARVSFQAGVRTAWVEDLLG
                     QRLGSLPVSGDEIELDLAPYALATLGIETGG"
     gene            5308..5553
                     /locus_tag="PLY56_01720"
     CDS             5308..5553
                     /locus_tag="PLY56_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HOJ20227.1"
                     /translation="MIDLFRSEDDHRIGQISEEALEFLIAHLEEEDSEDTDYYIDRDT
                     LEFLRDQNPPQGLVDILESALDGRDGIEVYYQAQEVP"
     gene            complement(5567..5980)
                     /locus_tag="PLY56_01725"
     CDS             complement(5567..5980)
                     /locus_tag="PLY56_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20228.1"
                     /translation="MGDPVRILVADDEQNLCRILVARLTRDGYLAEAVHDGVQALDRI
                     RRNSFDLLFLDLRMPALPGLDLLRRVREEHHPVVIVVMTAYDTPETVHAVLASGADAY
                     ITKPFDLDAVAESIPEWLSQRRTASRAALPDTGEE"
     gene            6245..9037
                     /locus_tag="PLY56_01730"
     CDS             6245..9037
                     /locus_tag="PLY56_01730"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOJ20229.1"
                     /translation="MALVLGAVFFGSELIRLYTDWLWFLDVGYRDVFVKTTVIKVGLM
                     VAAALLFFALIYANLLVAERLTPRPALYLDDQRQFRLTLTPFGRQGLSIILLIVAAAI
                     SLTVGLAAAKGWYDYLFFRNPVPFNQADPIFHRDVGFYVFQLPFVRFVYRWLMVGLGL
                     SLFAVAGLYWFDKGLDFVNGYVRIATHARVHLSALLAAILLTKAWGYWLARYELLFTP
                     HRLFFGAGFVDVHIRLVVLNVLVVLAVVAGLVMLANIRLRGVRLPMIIVGAWIAVSFV
                     GGIVLPSVVQKVRVTPNELSAEEKFISNSIEATRNAFGLSKIQRRSFPAAGALKMADI
                     RDNHETMRSIRLWDYEPLLDAYRQIQTIRAYYTFRGADVDRYQFPDGVRQVAISVREL
                     DVKRLGGAESSWINRHLIYTHGYGLCMSPVDRVAEGGLPELIIKDFPPQAPEALQLTR
                     PQVYFGELTNDYAIVNTTEEEFDYPSGDENMTTRYESDRGVRIAGLFRRLMFALRFGD
                     QNILLSTHLNADSRILYSRNIQERARRIAPFLRLDSDPYPVIHQGRIVWIIDAYTTSP
                     WYPYSQRIQPDPPRWDTVLNYMRNSVKITVDAYDGTVTLYVADESDPIITSLQKAFPG
                     LFRPLSEMPEALHAHLRYPKDLFNFQASIYAGYHMTDPRVFYNREDMWQVPRLQRDTT
                     DDLGLPIKSPPMAPYYMVMRLPDGDGAEYILMLPFTPTGDKQNMISWMAARCDPEHLG
                     ELVVYNFPKQKLVYGPAQIDALIDQHPRISEKLTLWSQSGSEVIRAPVMVIPIEDSIL
                     YVEPLYLRARNSRIPELKQVIVAYGSQVEMTDTLEESLRRIFGGDSTHSVAAAASTPP
                     ASRPTPAASPAPAGRPVDTPSPGISEEMRRLIDAASAQFERAQDAMRKGDWTTYGNEM
                     KQLQQSLERLRQVSSQ"
BASE COUNT         1600 a         3113 c         2960 g         1657 t
ORIGIN      
        1 ctccacgcgt ttcccgtcaa gcgtggctcc ggggaccgcg ccgcgctccg gcgcgcggag
       61 cagcttctaa gccagggcga gctggtcgtc atcttcccgg agggccgcga gagtgaaacg
      121 ggcgaactca tcgcgtttca gccaggcgcc gcgctgctgt ctctgcgcgc gggggttccg
      181 gtgattccga tctgcctgaa gggcaccgat ggcgtgatgc cctacggcac cgtcatcccc
      241 cggcgatccc cgcggccgac ggtcgttcgc atcggccccc cggtctgtct ggacgactta
      301 cgcgatcacc cggatcggag gcaggcgttg atggagggtg ccgagcgcat ccgcgccgcc
      361 gtggctgcgc tcgccgatgg ctagctgaca ttccagctga atagagaggc cctatggaga
      421 tacacgagat gcaggcggag gttgaccggt ggatcagcca gtttgaggaa ggctactggc
      481 atcccctctc gcaactggca cggctcaccg aggaggtggg cgagcttgcg cgcgagatca
      541 accaccgctt tggcgaaaag ccgaagaagc ccgaagaggc ggaggcaaac ctggcgctcg
      601 agatcgccga tgtcctcttc atcctggtcg ccctcgccaa ctcccagggg atcgatctag
      661 agcgtgcctt ccgtgaaatg atggagaagt accgggctcg tgatagcgag cgctggacgc
      721 cgcgctccac ccccaccccg gagccgccgg agagctcgga gcctactcca caatgatctc
      781 gacgttggca cgtgggatga caacgtgctc ccctccgtcg agatccgctc ccagaacgcg
      841 gacgctggca cccgtcggaa tctgctgcgg ctccggcggc agcgtagcca cgcgtccgag
      901 ccggccgaaa tacggctcgc ggatgacgcg gatgcgcgtg cctatctcca gggcactctc
      961 atcctcggcg gtcgtcatgc ctgcctgcgc ctcctgcgtg cgtggcacga tgatctctgg
     1021 acggatgacg cccgcgcgga tctgggttgc cccgttgatc gacgcgcgcc ggccatccag
     1081 tgaccgcaac agctcgaagg tgcgctcggc catgcgaatc tcgccgaacc cctccgtcac
     1141 catcagcgtg aggtggatat tctcatgccc ggtgattgcg acaccgatgt cgtgcccgag
     1201 gaactccatc aactcctggt cgatgatgcc tccggccacg atccccgcgg cgccggcctt
     1261 ggctgccgcg cgcagcgccg ctccagtgac cagagagccc ccgatcagga tctcgcccgc
     1321 gcactccgcg ggcacctgcg cctcatccag cggttgatcc ggaccggaga caaccacgcg
     1381 caacttcccc tcgcgctcgc cgccaactcc gaagatcccc tggatgaacg ccccgcgcgc
     1441 ctcgacgacc acgccctccc cgggaagcac ctccacgatg gtgccctcca cataggccgg
     1501 cacctccacg ggaatcggcg cctctcggat cgtgatgttg ccagtggtgt ccgacacaga
     1561 ctcgatagtg cccgagatgg gagcggtgac cgtggcgcga aacagtccga agaaagagcg
     1621 cgtctccgcg agcaactcgc ccttctgcac ggaatcgcca gcctgcttca agagggcgcg
     1681 ccagatctca cgcggctcga tgcccagcat ctgcgcggcg cgcaccatct gggcgtttcc
     1741 ggggagctcg gtgcgcgcga ccggcgtgct tggctccacg tggtcgcccg gggccaccaa
     1801 cacctggccc ttcaacggca gtctccgcgt cttctggatc gtaataaagg ggctcacctt
     1861 aagcccgggg gtgtatgcac ttcccattca cgcctcttcc actacccaca atgccgccaa
     1921 ccacaacaac aggctctggg atcactctct cagcgcagcg cccctattat agcacaatgc
     1981 ttcgcccttc gtcatccagg ccttgggatc cctgagattc cgacataggg agaaggccgc
     2041 cctcacgtgc ggtgatttat cgccgcgccg ggttgcgccc tgcctccgag gcttcggatt
     2101 ccttatgtcg gaatctcagt gggatcccgt aacgggacac cagttcccgg cacagagact
     2161 ccagtactcg acggccgggt ctctccggcc cccgttgccg tgaccccacg ggaagcctcc
     2221 cgcgtgattc ggccacaatc ccgggtgggg cggacatccc agggtgttcg caccctgcct
     2281 atccgtgtcc gtccccctca tccgcggtat cgacgcaggc cgggcgctat cctcccgtct
     2341 cgatgccgag ggttgccaga gcgtaaggcg ccaggtcgag ctcgatctcg tctccggaaa
     2401 cggggagcga gccgaggcgc tgcccgagga gatcctccac ccaggcggtg cgaacccctg
     2461 cctggaagga gacacgggcg cgctgggccc ggccctctgt ctcctccagc cgcaaccgca
     2521 ccatacccgg tcccaaccgg cgcagggaga ggagccgcac cgattccggc gtgatccgga
     2581 taaaaccacg ggccggccgg cggtcgtcgc ccagtggatt cggagcgcgc atctccgcga
     2641 agagcggctg ctggagcgcg gcggcggccc ggcgtgccgc gatgctatcc aggcccccgc
     2701 ccggacggaa gtggatcgag taggtgaagg tgtccaggag ctcctgccac aaagcgcagt
     2761 cggtatcgag caggttgtgg tagagccaac catagaggtg actgctcccg gcggtgaagg
     2821 ggagcgtctc gagccactcg ccggtgcgga tacgcccgaa ctggaagacg ggggcgtcgg
     2881 gcgaggtgac ggtgatgccg ccggtctcgc tggccagatc aatccacgac gggcagtaga
     2941 aggcatcctg catcgctccc ggaaggagat cctccgcggg ttgcacccag ccaatcgcct
     3001 gctcgaaacg gaactcggcg ttcccgccgg caaaggggag ggccacatag cagcactcct
     3061 tcgccggcgt ctccggctta tccacccgga tacgtagatc gattcgcgga gcgtgcgcgt
     3121 agaggatcac ctcgcgcgtc atccgaatcg ccccgctcgg tccctcgatc acgccgtgcg
     3181 cgaccacgct ctgcgccaca ggtccccaca ggccaatgcg ccagcccgcc gggcgctccg
     3241 ggatggcgac ccgcggccac tcccggccca tccccgcctt tcccgtgcca aaggcaaccg
     3301 cttcataccg gtgatagaga agctcgccga agccgatgcc gctcgcctga tccagacgct
     3361 ctcgcccgct ccgcttgtcg tagatcgacg tcacggctcc aatctcggag tcgatgcgga
     3421 ggcgcaggta gtcgttctca agctccaggc gcggctcgct gctcccggaa tcgggaagcg
     3481 tgccgaagcg ggggatcagg cgccggtagc caaacgcggg cacggtgggc gcgcgcagga
     3541 gatagcgccg gttgccgata cgctggcagg ggactgcctc gcccgtctcg gcatcggtca
     3601 ggcccgcgag ttccgggccg cctcgccata gataaagctc caccggctcg gaaagcggca
     3661 ccgtggcggg gttccacacc aggacgaacg gctctgtgct gtgtactgtc gcgcccaggc
     3721 gctggcgcgc ggcgcgttcc agaaatgcgg cgccctggcg tgcggcctcg gcatagaccc
     3781 gcttgcacgc ccacgactcg cggtggcggt cataggccgc ggcgcgggtg gaagcttctg
     3841 ggtcgagcga gttctcccat ccccacgcgc tcagaccgta ggtgtggtcg gagtagagct
     3901 gcaggttggc atggacttct tccacgcgtg ccggctcccc cggatcgggc gatggctcgc
     3961 gctcgaacgc ggcataggcc tcggcggcct ccagagtgcg ctgtgcctgg cgatacttct
     4021 tcagtgggct cggggttgga ttggcgcgca tcacccaggg atccgtcagg acaccgcgca
     4081 ggaccgggat gctctcgcca taccgagctt ccatcttccg gaggaaatca cacggtgtcg
     4141 tgagccggat gggcggagtc tcgcccgtgg cgttgatccg cgcgatcaga tcgacgaggc
     4201 cctcatcggg cccgccgtta tcgttcgcgt actgaagcat gacttcatcg aacggatagc
     4261 cgtcgttttc caggcgcgtc acaagctggg caaaccgctc gcgggcagtc ggcacgcctt
     4321 cgcgcctgcc tcgcgccagc tcctcgcgat agggcgggat caggtggata gaggccaggc
     4381 catacacggc gcggatcgcg gtcagttcct gcgggcgcac ctgccggtcc tgccccagat
     4441 tccagacgag gacccgggcg ccatccttgc tctcgagata gaagagatac ggcagatcgg
     4501 cccacggtag gtgcacctgg aagccgccca ccccggcaat taggtagcgg atgccaaacc
     4561 cttgcaggag ggtgggtagc cggcctgcgt agcctccgat gtcgtcgatg gtggccgctt
     4621 cgagcggaaa gccctcctgg cgcgccagct ccgcggcgta tccgatgcag cggatcaact
     4681 cctcggcgca tgccagctcc gtgagcggct gagtcaggca ggcctggagc tccacccagc
     4741 cggcgcgcag ggcgtgcagc aaccgctcgc ggtgcgcgcg tggccggcag cgtaggtact
     4801 cctgcaccag aagcgccgac tcgcacgtcc agcggaactg gcggtcctcg ggaaggtccg
     4861 cgttgccctg gcagagaacg agggcccgat ccagatgtgc gaggtgctgc tccagcactt
     4921 cgcccggcag atcggtgtag ccgatatcca tgtgcgtgtg gtggatgagg tgaaccgcgc
     4981 tcaatttcat gcgagaggag ttcccgcgcg gatttcgaat ctccttcccc acagtctgag
     5041 attccgggaa aggaatccga agcctcggag gcagcgcgca acccggcgcg cggcgataga
     5101 tcaccgcgct atttgcaagc gaagccctgc tgggctggat tggggatact cccagatgtc
     5161 tcaaaacggc ggtggctggg aggagaagcc cgaaggggct ttgccagaaa gtagcgcgga
     5221 gcctcggctc cgcgcgtgcg ggcggccatc tcctttttcg gaatcccaga gccacggcag
     5281 tcgcaatctc gtcgagaggg gaacgccatg atcgacctgt ttcgctctga ggatgatcac
     5341 cggatcggcc agatcagcga ggaagccctc gagtttctta tcgcccacct ggaggaggaa
     5401 gacagcgagg acacggatta ctacatcgac cgcgacaccc tggagttctt gagggaccag
     5461 aacccgcccc aggggctggt cgatatccta gaaagcgcct tggatggtcg cgacgggatc
     5521 gaggtctatt accaggcgca ggaagtgccc tgaatgcggg gcgaggctac tcttcgcccg
     5581 tgtctggtag agccgctctg gatgccgtgc gccgctgaga tagccattcg gggatggatt
     5641 cggccaccgc gtccagatcg aacggcttgg tgatgtaggc atcggcgccg ctggccaaga
     5701 cggcatgcac cgtctcgggg gtgtcgtagg cggtcatgac gacgatcacc accgggtggt
     5761 gctcctcgcg aacgcgcctg agaagatcga gccccgggag cgcgggcatg cgcaggtcaa
     5821 ggaagaggag atcgaagctg tttcgccgga tgcggtccag tgcctggact ccatcatgca
     5881 ccgcctctgc aagataccca tcgcgagtca gccgagccac caggatacgg cataggttct
     5941 gctcatcgtc tgccaccaga atccgcaccg ggtcacccac ccgtctctct tcccctctct
     6001 tgccctcgca ccggggcgag cctcccggtg gctccgtgat ctctcactgc gcgttctctc
     6061 ggaacgcgag cgggcatccg accggcctgc gaattagaaa ccgcaagatc ggtgccatcg
     6121 ctcttgggat ctggagattg acgccccacc ccacggaggg tataatgggg ccaccatgaa
     6181 cagtaagtcg tatctagggg attcgtggag agaagggttc cgccgcgtcc tttggatcgc
     6241 gggcatggcg ctggtgctcg gggccgtgtt cttcggatcc gagttgattc gcctgtacac
     6301 cgactggctg tggttcctgg atgtgggcta tcgcgacgtg ttcgtgaaga ccaccgtgat
     6361 caaggtcggg ctcatggtcg ccgcagccct gctctttttc gcgctgatct acgcgaacct
     6421 tctggtggcc gagcgtctca ccccgcgccc ggcgctctat ctggatgacc aacgccagtt
     6481 tcggttgacg ctcacaccgt tcggccggca gggactttcc atcatcctct tgatcgttgc
     6541 ggctgctatc agcctcaccg tcggcctggc agcggcgaag gggtggtatg actacctctt
     6601 cttccgcaac ccggtgccgt tcaatcaggc ggatccaatc ttccaccgcg acgtcggctt
     6661 ctacgtcttc cagcttccct ttgtgcgttt tgtctatcgc tggctcatgg tggggctggg
     6721 cctcagcctc ttcgccgtcg cggggctcta ctggttcgat aagggactgg atttcgtcaa
     6781 tggctacgtg cgaattgcca cgcacgcgcg cgttcatctc tcggcgctgc tggccgcgat
     6841 cctcctgacc aaggcctggg gctattggct ggcacgctat gagctgctgt ttactcccca
     6901 ccggcttttc ttcggcgctg gcttcgtgga tgtgcatatc cgcctggtgg tgctcaacgt
     6961 cctggtcgtg ctggctgtag tggccggcct ggtgatgctg gccaatatcc gcttgcgtgg
     7021 cgtgcgtctc ccaatgataa tcgtgggggc gtggatcgcc gtctcgttcg tgggcggcat
     7081 cgtcctgcca agcgtggtgc agaaggtgcg cgtcacgccg aacgagctga gcgccgaaga
     7141 gaagttcatc agcaacagca tcgaggcgac gcgcaatgcc tttggactct ccaagatcca
     7201 gcggcgcagc ttccccgcgg ccggcgccct gaagatggcg gatatccgcg acaaccacga
     7261 gacgatgcgc agcatccgcc tgtgggacta cgagccgctt ctcgatgcct accgtcagat
     7321 tcagacgatc cgtgcctact acaccttccg tggcgccgat gtggatcggt atcagtttcc
     7381 agatggcgtg cgccaggtgg ctatctcggt gcgcgagctg gacgtgaagc ggttgggggg
     7441 cgcggagagc agctggatca accggcacct catctatacg cacggctacg gcctctgcat
     7501 gagccccgtc gaccgcgtgg ccgagggcgg cctgccggag ctcattatta aggatttccc
     7561 gccccaggcg cctgaagccc tccagctgac gcggccgcag gtctacttcg gagagttgac
     7621 caacgattac gccatcgtca acacgaccga ggaggagttt gactacccgt ccggcgatga
     7681 gaacatgacc acgcgctacg agtccgaccg tggcgtccgg atcgccgggc tcttccggcg
     7741 cctcatgttc gccctgcgct tcggcgatca gaacatcctg ctcagcaccc atctgaacgc
     7801 tgatagtcgg atcctgtaca gccgcaatat tcaggagcgt gctcggcgca tcgcgccctt
     7861 cctgcggctg gatagcgacc cgtatccagt catccaccag ggccgcatcg tctggatcat
     7921 cgatgcctac accaccagcc cgtggtatcc ttactcccag cgcatccagc ccgacccgcc
     7981 gcggtgggat acggtgctca actacatgcg caactcggtg aagatcaccg tcgatgccta
     8041 cgatggcacc gtcacgctct acgtggccga cgagagcgat ccgatcatca ccagcctgca
     8101 gaaggcattc cccggattgt tccggccgct gagcgagatg cccgaggcgt tgcacgcgca
     8161 cctgcgctat ccgaaggatc tctttaactt ccaggcgtcg atctacgccg gctaccacat
     8221 gactgacccg cgcgtcttct ataaccggga ggatatgtgg caggtgccgc ggttgcagcg
     8281 cgatacgacc gatgacctgg gcctgcccat caagagccct cccatggcgc cgtactacat
     8341 ggtcatgagg ctcccggatg gcgatggggc cgagtacatc ctgatgctgc cgttcacgcc
     8401 gaccggtgac aagcagaaca tgatctcgtg gatggcggca cggtgcgatc ccgagcatct
     8461 cggcgagctg gtggtgtata acttccccaa gcagaagctg gtctatggtc ccgcgcagat
     8521 cgacgcgttg atcgaccagc acccgcggat ctccgaaaaa ctgacgctgt ggagccagag
     8581 cggctcggag gtcatccgcg cgcccgttat ggtcattccg atcgaggact cgatcctcta
     8641 cgtggagccg ctctacctgc gcgcgaggaa tagccgcatc ccggagctga agcaagtgat
     8701 cgtggcctac gggagccagg tggagatgac cgataccctg gaggagagcc tccggcggat
     8761 cttcgggggc gattccacgc acagcgtcgc ggccgccgca agcacacctc ctgcgagccg
     8821 gccaacgccc gctgcgtcgc cggcgcccgc gggccggcct gtggacacgc cctcgccggg
     8881 catctccgaa gagatgcggc gcttgatcga tgctgcctcg gcgcaattcg agcgcgccca
     8941 ggatgccatg cgcaagggcg attggaccac ctatggcaat gagatgaagc agctccagca
     9001 atcgcttgag cggctgcgcc aggtctcctc gcagtagttc tctggggtcg ggggtgggaa
     9061 aaggcccgcc cccgacccac ctgccggcca ctgccggtga cggctcccgc gaccccctgt
     9121 ttcatgaagt gtgctcggca cactctcttt tcgccccctc gctgatccgg cgcgtatcgc
     9181 acctcttccc tggcgtctca cacgggcgct gaggcccttc gtgatgccca acaggggcac
     9241 ccccactctg tttgacccct caccccaccg ggaaatctgc gcggtagcga atgcggcagt
     9301 gaggggggca gatgatacgc aggtgcatgg
//
LOCUS       DAOSCV010000036         9263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4889_length_9263_cov_3.135317, whole genome shotgun sequence.
ACCESSION   DAOSCV010000036 DAOSCV010000000
VERSION     DAOSCV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_9263_cov_3.135317"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1740)
                     /locus_tag="PLY56_01735"
     CDS             complement(<1..1740)
                     /locus_tag="PLY56_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20230.1"
                     /translation="MFMALTASSGSAAPESKILLVASSPENYVARGLNALGVAHERAS
                     ASDYRNRSPFDYDILIWGFDEDRAALATDPGAIRAFVEGGGIFLGMRCSNEDTWLPSP
                     MKRDKAYQFGKILQPDHPIFNTPHRMDQKAMLDVHSGSIYRAFYDLGEGWTPLVSTGA
                     EQNWDQVEARSKGEHYGIVELALGKGRILLVQMIPEYHWFSDAKGDRSSAGARFFENL
                     IQYALSKSSRQAASRPPRKRPDRFYADLSEALALPRRGDGVPVNDGKWRFTSAGPYSM
                     KTDRRGVITLTHADEPSKAGNFAQVTRTIPIVASDAPVTLRWYESDTYCGGREIVLGG
                     AKHGQTALENYKREMRFAQVLVNGEKVWEEDVLGRNPQPARTRFRTADITEIVRKAGG
                     RCEVTLRVEDRQGSGEHPFAIDVFFATVDVITDLRRASAAALLKGNGFTPGNEGSLVL
                     RAGSGAAQTTHAGSAGRFAIAFSVRDEHTGRSRVRVMAGRQEVARWTLTADDHRVYWA
                     VTPPVRLAPGTPIRIEATAVGDETVEIREVAVIPERLLAKPIVAAAPPADGGAGAKRV
                     RFQVTVPETAGIAR"
     gene            2099..4765
                     /locus_tag="PLY56_01740"
     CDS             2099..4765
                     /locus_tag="PLY56_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20231.1"
                     /translation="MKYACLILALITGAAHAAEVPVIPEEIAIPPGETRAFEFGTLPQ
                     ADTTILLEVQARLDAKAFSGSMFFLRVRLNGKEVKAAKSRTALRLVNKPLVSPVAHDL
                     PAAWYGTGGWRVVYGPDFEGARQQRFYEGDPYTLVLDVTDLTNPAAENRLEITNTANP
                     NSIRYAGTEGNLVISKLTVRTQPGKSPTMTGSDAVAPVINNGQPGAGPAAYQGELLPG
                     GGFALTVGGRRWEFASDFSYPNAGLNRLTAAAAPDTTGQPGWKVDARPGKAGGTVVAS
                     GPDYRVRRTVRFTARKVEVEDAITNAHADAPLGMLVRHQTSLEALENPVVRLAGNPDP
                     AVDDYYSPSNPSVYVAMADYALGMLCEDDVFRNQARLYCTTDPPAAGIRTEMLRLGPG
                     ETYTLRWSIYPVASRDYYDFINLVREDWGSNFTVLGAWTFFSPDAILAMPLEEIREQY
                     RRQGIRYACSYGGWVDAKHDRKRIGFGTGVLDDYWADFRGRLKAAAARIREACPEVKV
                     LVYYDSQRDTSEGGHERFKDSWLTNTKGEQLSTEWSGQYSLTYSVVATVQNSYGKAML
                     TVADRYLDEIGADGLYWDEMECVGYGAPLLTYNILDGHSCILDPKSYTIQREVGITTL
                     LGEGHRLAVIDRVRKRGGFLMGNGPACTRAILATGVQRMVEIQHNDYWFYEGLLGTPL
                     GYASGRTDFGNVTRALRFGFLLVGTRHNYPHEISRYLFPFTPIELHHGYLLGKERIIT
                     LHPGKYGWPGERCLVQVRHFNREGMLTETDYPTTVEGESRTAIQPAEEEVVVLERLPV
                     TLDPRGGKAEVSRVRYSAEGLALSVTAPRGATLRIATGAFEIRPGARYSVRIGSQPAR
                     TVTPEKGILTVVIPAGAATPVTVSPAGAPVPG"
     gene            complement(5015..5497)
                     /locus_tag="PLY56_01745"
     CDS             complement(5015..5497)
                     /locus_tag="PLY56_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20232.1"
                     /translation="MARFKRGDRVVVTPRQVTAADRKSGLYYAHFAGLHGTVDQVYEE
                     EGEVCVEVDQDSLPESFERRHLGIQEYVQQRWLNGISEHERRNLPEEQRSLRLKYTIM
                     VAPDDLVPEGQAKKSRPAEKAAPGSGEGTKPATPRLQETDLTAAEEAYFEEIRRRANQ
                     "
     gene            6079..7776
                     /locus_tag="PLY56_01750"
     CDS             6079..7776
                     /locus_tag="PLY56_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20233.1"
                     /translation="MTIRIVLTLALALAVTLPAAAQQGASDQNTGTMPSATTTMSADT
                     FRQGAFVSYQAATADRPGSVTLRFNWQGMDILGTYEVQPGQSWMVNGRQVNLSTVAKN
                     TMVTIPQDARLTHLTFGGTVGVGTITAMNPTQRTLTVRLPSGETRMIRHAENLLVVRN
                     GQIMSVTQLAPGQTVLFEVPPDGNQVVILREVGTGTIATVTDVTDDSVTLRFAQAGQQ
                     MTRTYPLDEGIIVFHQGRMVDVEDVEDRLATNPQVWIFGPIDNPHLVVSQPTMGFETA
                     VVTIEETEVATVPTTPEMPVAVQAMAPPFNEGTFVSYSPATDTMPALATFRFNWQGTP
                     LLGTFMVREEDRFLSDGQLVNLADLEEGAMVTVPQGARLANLTFGGNVGIGTISSVDR
                     SRNTVSVRLPNGETRTLRYSPEAIFLENGQPASIAELDTGQTVLFEVVPQRNVVGIFT
                     PVGEGMQATVTQITDEGITVRYVMNGQMVTRTLTSDEVAFVNRGQAVSDLAGAYRVGQ
                     TVWIYGPADNPQLVAGGVNMGFETAVVTQPSPAVAGVREEPAAAPAQPARPVIRGRW"
     gene            7887..8609
                     /locus_tag="PLY56_01755"
     CDS             7887..8609
                     /locus_tag="PLY56_01755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ20234.1"
                     /translation="MAIRPLVAVGGIGVVLALCLASAAAREPSRPPGEATASRARIVI
                     NIPSRTLTLFLDERVYKTYPVGVGRPQWPTPLGHWRILEKVINPVWGYPLDFDPDGRR
                     GVIPPGPRNPLGTRWMGFTPEEHGIHGTNQPKSIGKVISHGCVRMHIPDSEELFDLVE
                     VGTPVEVRHDTVLVKIDRARGYVLASSYPDVYRKGGSTRAMVEEALKQQRVQARLSDE
                     RLRKLRSRRDGVYRVVGWLIYP"
     gene            complement(8649..>9263)
                     /locus_tag="PLY56_01760"
     CDS             complement(8649..>9263)
                     /locus_tag="PLY56_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20235.1"
                     /translation="RVEPTFEDEPVSAPPIPSSAESDLALAAADAARPSGSTPPAEAA
                     SDAPSTPKVDLAAEVAEAEKPAESPAPAETALDAPPTLVADLAPKAAEAERPAESRTA
                     DEATPARVENAGGSAAISAREAAVLFAHDAIHDRAVAGERVVCLAVGDVREVELEGGR
                     AHIIVPLELSHRSEGRLPQTRAATLHVLLEGGPTDWRIASHSWE"
BASE COUNT         1577 a         3047 c         3093 g         1546 t
ORIGIN      
        1 tcgcgcgatg ccggccgtct ccggcacggt cacctggaag cgcactcgct tggcgcccgc
       61 gccgccatcc gccgggggag ccgccgccac gataggcttt gccagcagac gctccgggat
      121 cactgctacc tcgcgaatct caaccgtctc atcgccgacg gcggttgcct caatccgaat
      181 cggagtgccg ggcgccagcc tcacgggcgg cgtcaccgcc cagtacacgc ggtggtcatc
      241 cgccgtgagc gtccatcggg ccacctcttg ccggccggcc atgacccgca cccgcgaccg
      301 cccggtatgc tcgtcgcgca ccgagaaggc aatggcaaac cggcccgcgc tgccggcgtg
      361 cgtggtctgc gcggccccgg agccggcgcg caggaccagg ctgccctcgt tgcccggcgt
      421 aaagccgttc cctttcagga gagccgccgc cgaggcgcgc cgcaggtcgg tgatcacatc
      481 gacggtggcg aagaagacat cgatcgcgaa gggatgctcg cccgatccct ggcgatcctc
      541 gacgcggagc gtcacctcgc agcgaccgcc cgccttgcgc acgatctcgg tgatatcggc
      601 cgttcggaag cgcgtccgcg ccggctgggg attgcgcccc agcacatcct cttcccacac
      661 cttctcgccg ttgaccaaca cctgggcgaa gcgcatctcg cgcttgtagt tctccagcgc
      721 cgtctgtcca tgctttgcgc caccgagaac gatctctctt ccgccgcagt aggtgtcgga
      781 ctcgtaccag cgcagggtga cgggagcgtc gctggcgact atcgggatcg tgcgcgtcac
      841 ctgggcgaag ttgcctgcct tgctcggctc gtccgcgtga gtgagggtaa tcacaccacg
      901 ccggtctgtc ttcatcgagt aggggccggc cgaggtgaag cgccacttgc cgtcgtttac
      961 gggaacgcca tcgccccggc gcgggagcgc cagcgcctcg gagagatcgg catagaagcg
     1021 atccggccgc ttccggggcg ggcgcgaggc ggcctggcga gagctctttg acagggcgta
     1081 ttgaatgagg ttctcaaaga agcgcgcgcc ggccgaagag cgatccccct tcgcgtcgga
     1141 gaaccagtgg tattccggga tcatctgcac caggaggatc cgacccttgc cgagggcgag
     1201 ttctacgatg ccgtaatgct ctcccttgga ccgcgcctct acctggtccc agttctgctc
     1261 tgctcccgtg ctcaccaggg gagtccagcc ttctcccaga tcgtagaagg cgcggtagat
     1321 gctgcccgag tgcacatcca gcatcgcctt ctgatccatg cggtgcggtg tgttgaaaat
     1381 cgggtgatcc ggctggagga tcttgccaaa ctggtaggct ttatcgcgct tcatcgggga
     1441 tggaagccag gtgtcttcgt tgctacagcg catccccagg aagatgcccc ctccctcaac
     1501 gaacgcgcgg atcgctcccg gatcggtcgc gagcgcagcg cggtcctcat caaagcccca
     1561 aatgaggatg tcgtaatcga agggcgaacg gttgcggtaa tcgctcgcgc tcgcgcgctc
     1621 gtgggcgacg ccaagggcgt tcaaaccccg agcgacgtag ttttcgggcg agctcgccac
     1681 caggagtatc ttcgactccg gagcggcgct gccactgctc gcggtcagtg ccatgaacat
     1741 cgccatcatc ccccacacgg cgaggcgcca agcatgccgc cgtgcgctca gtcgaagcgg
     1801 tgaacaagac ttcatggtgc gcatgaacat acccttgtcc tcgattgatc ccgcggctgg
     1861 gcccgccccc gagggtgccc gccgtacctt accgccttac ttccacagca cggcgggcat
     1921 tcctgcgcca ggcgcgcgga gggtgcgccc cgagagatcg cctccatgcc gccggcggca
     1981 ccgcggataa ccggggaaga ggagacgccc cctcgcgagc gaagaagggg ctagtgagtg
     2041 cgccactggt ggcttgcgcc tcacatctca tcgattgtca atcgcgcgga ggagcttgat
     2101 gaagtacgca tgtctgatcc tggccctgat caccggggcc gcgcacgccg cggaggtacc
     2161 ggtgatcccc gaggagatcg ccatcccgcc cggcgaaaca cgcgccttcg agtttggcac
     2221 tttgccgcag gcggatacga cgattctcct ggaggtgcag gcgcgcctgg atgccaaggc
     2281 gttctctggc tccatgttct tcctccgtgt gcgcctcaac ggcaaagagg tgaaagcggc
     2341 gaaaagccgg acggcgcttc gcctggtgaa taagccactc gtctcgccgg ttgcccacga
     2401 tcttcccgcg gcgtggtacg gcaccggcgg gtggcgcgtc gtctacggcc ccgatttcga
     2461 gggcgcgcgc cagcagcgat tctatgaggg agacccctat acgctcgttc tggacgtgac
     2521 cgatctcacc aatccggctg cggagaaccg gctggagatc accaacacgg ccaatccgaa
     2581 ctcgatccgg tatgccggaa ccgaggggaa tctcgtcatc agcaagctca cggtgcgcac
     2641 gcagccggga aagagtccca ccatgaccgg ttctgacgcg gttgcccccg tgatcaacaa
     2701 cgggcagcca ggcgccggac cggcggccta ccagggcgag ttgcttcccg gaggtggctt
     2761 tgccctcacc gtgggtggcc ggcgctggga gtttgcgagc gacttctcct atccgaatgc
     2821 cggcctcaac cggctcacgg ctgccgccgc cccggatacc acgggccaac ccggctggaa
     2881 ggtagacgcg cgccccggca aagcgggcgg caccgtcgtc gcgtctgggc ctgactaccg
     2941 cgtgcggcgc acggtccgct tcaccgcgcg caaggtggag gtggaagacg cgatcaccaa
     3001 cgcgcacgcg gacgcccccc tggggatgct ggtgcgccac cagacgagcc tggaagcact
     3061 tgagaacccc gtggtgcgcc tagccggcaa ccccgacccg gcggtggatg actactactc
     3121 gccgtccaac cccagcgtct acgtggcgat ggcggactac gctctcggca tgctgtgcga
     3181 ggatgacgtc ttccgcaacc aggcccgcct ctactgcacc acggatcccc ctgccgcggg
     3241 catccgcacg gagatgctcc gcctgggccc gggagagact tataccctgc gctggtcgat
     3301 ctatcccgtg gcctcgcgcg actattacga cttcatcaac ctagtccgcg aggactgggg
     3361 ctcgaacttc acggtgctgg gcgcctggac cttcttcagt ccggatgcga tcctggcgat
     3421 gccgctggag gagatccgcg agcagtatcg ccgccaggga atccgctacg cctgctctta
     3481 cggcgggtgg gtagatgcca agcatgatcg caagcgcatt ggcttcggca caggcgtact
     3541 cgacgactac tgggccgatt tccgcggccg gctgaaggca gcggcggcgc gcatccgtga
     3601 ggcctgcccc gaggtgaagg tcctcgtcta ctacgatagc cagcgcgata cttccgaagg
     3661 cgggcacgag cgtttcaagg atagctggct cacgaacacc aagggcgagc agctctccac
     3721 cgagtggagt ggccaatact cgctcaccta cagcgtagtc gcgacggtgc aaaacagcta
     3781 cggcaaggcg atgctcaccg tggcggatcg ctatctggat gagattggcg cggacggcct
     3841 ttactgggac gagatggagt gcgtgggcta tggcgcaccg ctccttacct acaacatcct
     3901 tgatggccac tcgtgcattc tcgatccgaa gagctacacg atccagcggg aggtcggcat
     3961 cactacgctg ctgggcgagg gacaccgcct cgccgtgatt gatcgcgttc gcaagcgcgg
     4021 cggcttcttg atgggcaacg gcccggcgtg cacgcgggct atccttgcga cgggcgtgca
     4081 gcgcatggtc gagatccagc acaacgatta ctggttctat gaggggcttc taggtacgcc
     4141 cctgggctat gccagcggcc gcaccgattt cggaaacgtc acgcgcgcgc tccgctttgg
     4201 gtttcttctc gtcggcacgc gccacaacta cccgcatgaa atctcgcgct acctcttccc
     4261 gttcacgccc atcgagctgc accatggcta cttgctgggc aaggaacgaa tcatcacgct
     4321 gcatccgggg aagtatggct ggcctggaga gcgctgcctg gtgcaggtgc gccacttcaa
     4381 ccgcgaggga atgctgaccg agaccgacta ccccacgacg gtagaggggg aatcgcgcac
     4441 ggctatccag cccgcggagg aggaagtggt tgtcctggaa cgcctgccag tgacgctcga
     4501 cccgcgcggt ggaaaggcgg aggtctcgcg ggtgcgctac tccgcggagg gcctggcgct
     4561 gagcgtgact gccccgcgcg gggccacgct ccgaatcgcc accggggcgt ttgagatccg
     4621 cccgggggcg cggtattccg ttcgcatcgg ctcgcagccg gcgcgtaccg tcacgcccga
     4681 gaaggggatc ctcacggtcg tcatcccggc cggcgcggcg acgcccgtga ccgtctcgcc
     4741 cgccggggcg cctgtgccag gataggcatc gagccccagg cgcatcacac ggatctaaag
     4801 ctccgcgctg cttgtgagcg aagccctgcc gggccaggtt aggtgcggac tgggaggggg
     4861 agcctcgaaa ggggctttgc tgagaagcag cgcggagcta gaagctccgc gccccgggtg
     4921 ctgggctgcc ggcatcctcc ccatccgtgt ccgccccctt catccgtggt accgccgccg
     4981 gcgagcacag gagagcgggg gccagggaga ggggctactg gttggctcgg cgccggatct
     5041 cttcgaaata cgcctcttca gcggcggtga ggtccgtctc ctgcaggcgg ggggtagctg
     5101 gcttcgtgcc ctcgccgctc cccggcgccg ccttttcggc cggacgtgac ttcttcgcct
     5161 ggccctccgg aaccagatcg tcgggcgcga ccatgatggt gtacttcagg cggagagagc
     5221 gctgctcttc cgggagattg cggcgctcgt gctcggagat gccattcagc cagcgctgct
     5281 gcacatactc ctggatgccc agatgccggc gctcgaagct ttcaggaagc gagtcctgat
     5341 ccacctcgac gcacacctct ccctcttcct cgtacacctg atccaccgtg ccgtgcagac
     5401 ccgcgaagtg ggcgtagtag agcccagact tccggtcggc tgcggtcacc tgtcgcggag
     5461 tcacgacgac gcgatcacca cgcttgaaac gggccaagct gcttgctcct ttcctggtga
     5521 gacgggacct atcgcggtta cagatccgat gcggccgctt tgaccaaggg gcattgttcc
     5581 cctcgaccag ggtgttggga agcttcccaa ggggatgcct gtgccggcgc gatacgaaat
     5641 ccaaagagcg cgctggatcc tcgttctacc acgcactcta ttcggggcag acgggggcct
     5701 ctcctgccgc ggggagtggc aagatagcca gagacctgag ctgaggatcc gtgggggatc
     5761 gcgcgtgcgc gctgacggcc gcgtttagcc cgagggaggg ggtggtacat gccctcccgg
     5821 atgggcgcag taggccatcc cggctccagt attgggcgga gggttaggca tacagaacac
     5881 cgcaaggctg tcggagcgct acccgaaaga acgggctgag ggggctcctc catcggcgag
     5941 gcgagcggca gcgcggagtg gatggatgca tctgacccac cggcccagta aggagccgca
     6001 accagatgga gatccggcgc gtgggatgcc agggggagat agcctcgccc gcgcgtcagg
     6061 caggaggaga cagaggagat gacaatccgg atagtgctca ctctggcgct cgcccttgcg
     6121 gtgaccctcc cggcagcggc gcagcagggt gcgagcgatc agaataccgg aacgatgccg
     6181 tcagcgacca cgacgatgtc ggcggataca ttccgccagg gcgccttcgt gagctaccag
     6241 gcggcgactg cagatcgccc aggcagcgtg acactccgct tcaactggca agggatggat
     6301 atccttggca cctacgaggt gcagccgggg cagagctgga tggtgaacgg ccgccaggtg
     6361 aatctcagca ccgtcgccaa aaacacgatg gtgaccatcc cgcaggacgc gcgcctgacc
     6421 cacctcacgt tcggcggcac cgtgggcgtt ggcaccatca cggcgatgaa ccccacgcag
     6481 cgcacgctca ccgtgcgtct gccaagcggt gagacgcgca tgatccgcca tgccgagaac
     6541 ctgcttgtgg tgcgcaacgg ccaaatcatg tcggtgacgc agctcgcgcc aggccagact
     6601 gtcctctttg aggttccacc ggatggaaac caggtggtca tcctgcgcga ggtaggcacc
     6661 ggcacgattg ctacggtgac cgatgtcacg gatgattctg tgacgctgcg ctttgcccaa
     6721 gccggccagc agatgacccg cacctacccg ctcgatgagg ggattatcgt cttccaccag
     6781 ggtcgaatgg tggacgtgga agatgtggag gatcggctcg ccaccaatcc gcaggtgtgg
     6841 atcttcggcc ccatcgataa cccgcacctc gtcgtttcgc aacccaccat gggcttcgag
     6901 acggccgtcg tgacgatcga ggagaccgag gtagccacgg ttccgacgac gcccgagatg
     6961 ccggtggccg tccaggcgat ggcgccaccc ttcaacgagg gtaccttcgt ctcctattcg
     7021 cccgcgaccg acaccatgcc ggcgctggcg accttccgct tcaattggca gggcacgccc
     7081 ctgctgggca ccttcatggt ccgcgaggag gaccgattcc tgtccgatgg ccagctggtg
     7141 aaccttgccg accttgagga aggcgcgatg gtgacggtgc cgcaaggcgc gcgtctggcg
     7201 aacctgacgt tcggcgggaa tgtgggcatc ggcacgatca gctcggtgga tcgcagccgc
     7261 aacaccgtct cggtgcgcct gccgaacggt gaaacgcgca ccctgcgcta ctctccggaa
     7321 gcgatcttcc tggagaacgg gcagccggca agcattgccg agctggacac cgggcagaca
     7381 gtcctcttcg aggtggtgcc ccaacgcaac gtggtcggga tcttcacgcc ggttggcgag
     7441 ggaatgcagg ccacggtgac gcagatcacc gatgagggga tcaccgtgcg ctacgtgatg
     7501 aacgggcaga tggtgacccg gaccctcacc agcgacgagg tcgccttcgt gaaccggggc
     7561 caggcggtgt ccgatctggc gggcgcctac cgcgtgggcc agacggtctg gatctacggt
     7621 ccggccgaca acccgcaact ggtggccggc ggggtgaata tgggcttcga gacggcggtg
     7681 gtgacgcagc cgtcaccggc ggtggctggg gtgcgtgagg agccggcggc agcgcccgcg
     7741 caacccgcga ggccggttat ccgcggccgc tggtagaccg cgccaagctc gcttgcgggg
     7801 gtgcgctcca tccagggcgc gcccccgcgc gtttggggag cccggagcca agccgggcaa
     7861 gcaggatgga gaggagcctt ctgaagatgg caatccggcc actcgtcgca gtgggaggga
     7921 tcggggtggt gctcgcgctc tgcctggcat ccgccgcggc gcgagagccc tcgcgccctc
     7981 cgggcgaagc caccgcctcg cgggcgcgga tcgtcatcaa catcccctcg cgcacgctga
     8041 ccctcttcct ggacgagcgc gtttacaaaa cgtaccccgt cggagtgggc cggccccagt
     8101 ggccgacgcc gctcggccac tggcggatcc tggagaaggt gatcaatccg gtgtggggct
     8161 atccgctcga ttttgatccg gatgggcgcc ggggcgtgat cccaccggga ccacgcaacc
     8221 cgctgggcac acgttggatg ggcttcaccc ccgaggagca tggcatccac ggcaccaacc
     8281 agccgaaaag catcggaaag gtgatctcgc acggatgcgt gcggatgcat atcccggata
     8341 gcgaggagtt gttcgacctg gtggaagtgg gcacgccggt ggaagtgcgc cacgacaccg
     8401 ttctggtgaa gattgaccgg gcgcgcggct acgtgctggc ttccagttat cccgatgtct
     8461 accggaaggg cggttcgacg cgcgcgatgg tggaagaggc cttgaagcag cagcgtgttc
     8521 aggccaggct ctcggacgag cgcctgcgca agctccggag ccgccgcgat ggggtgtacc
     8581 gggtcgtcgg ctggctcatc tatccatagc gattccgggc gcgtgggtgg gctttgccgc
     8641 gctgagtctc actcccacga gtggctcgca atccgccaat ctgtcgggcc accctcgagg
     8701 agaacgtgca gcgtggccgc cctggtttgg ggcaggcgac cctcgctgcg gtgcgagagc
     8761 tcaagcggca cgatgatgtg ggcacgcccc ccttcaagct ccacttcacg gacatcgcca
     8821 acggccaggc acaccacacg ctcgccggcg acggcgcggt cgtggatggc atcgtgagca
     8881 aagagaaccg ctgcttcccg cgccgaaatc gccgctgatc cgcccgcgtt ctccacccgc
     8941 gcgggagtcg cctcgtcggc ggtgcggctt tcggccggcc tctccgcctc agctgccttg
     9001 ggtgcgaggt ccgccaccag agtaggggga gcgtcaagag cagtctcggc gggagcgggg
     9061 ctttcggccg gcttctccgc ttcagcgacc tcggcagcca ggtccacctt gggagtggaa
     9121 ggggcatcgg aagcggcctc agcgggaggg gtgctccccg acggtctcgc cgcatcggcg
     9181 gccgcgagtg cgagatccga ttccgcgctg gaagggatgg gcggtgccga gacgggctca
     9241 tcctcgaagg ttggttccac ccg
//
LOCUS       DAOSCV010000037         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_4982_length_9121_cov_3.818553, whole genome shotgun sequence.
ACCESSION   DAOSCV010000037 DAOSCV010000000
VERSION     DAOSCV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_9121_cov_3.818553"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..>1476)
                     /locus_tag="PLY56_01765"
     CDS             complement(275..>1476)
                     /locus_tag="PLY56_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOJ20236.1"
                     /translation="MEPPKPKKEFIEARGVYLTGWSAGGKQKMQKIVELLKATGMNAV
                     VIDVKDSDGAITFKMDIPWAKQVEEKRGQIKEINHYGYAQRCADIDWVMNLLKENNIY
                     PIARIAVFADDILPRVRPDMAAKNASGGIWENRKKEAWLDPYNKEGWDYNAAVAIEAM
                     KKGFKEIQWDYVRFPTDGKLGNLRFPAKTDTPPNEVIAQFLAYTRKKLEPYGAIVSAD
                     IFGLTGLVSDDMGIGQLIHQIAENVDYICPMVYPSHYHKGEYGIPNPDAAPYDIVYVS
                     LRDAQRRLAGTGCKIRPWLQNFSLPDRGRYGGSHYGPPQIAAQIKATFDRGIPEFLMW
                     DPKNRFNGLDAAIASLKQKGIKPRPTPPDDLKPPAKAPANAPAQPAGNAPAEAPGQPA
                     SNAPARR"
     assembly_gap    1477..1486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2057..3115
                     /locus_tag="PLY56_01770"
     CDS             2057..3115
                     /locus_tag="PLY56_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ20237.1"
                     /translation="MASRRSILSSILGISLAALLLGAGCQRQSSTTAPGPEPGQKPAQ
                     QSAAPAPAPPVSDAAPNELGRVPVLEYHVIGPKEGRWARTPEAFRKDLETLYAHGFRA
                     VSLRDYVTGNINLPRGASPVVFTFDDSTEGQFRYLEKDGQLVIDPDCAVGILEEFNRK
                     HPDFGFKATFYVLPESAFGQPQYRKQKLQYLAEKGLEIGNHTWSHTALKRLSNEKVQE
                     ELARPQQAIGEVVPNFRFFSLALPLGISPKEKALASKGAWNGITYEHHAVLLVGSVPA
                     PSPYDKDFDPRAIQRVQATDWPEVKPFQLSAQIKTLVETENRRYVSDGDPNTIAVPAA
                     LKDELAPERTQGKEVKIY"
     gene            complement(3203..4321)
                     /locus_tag="PLY56_01775"
     CDS             complement(3203..4321)
                     /locus_tag="PLY56_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ20238.1"
                     /translation="MIYRMLGRTGLRVSQLGFGCMRLPMKGGMTEGTVDRELAVPMLH
                     RAFEAGVNYVDTAVGYCNSDSQRAVGDALKGWRDRIIVSTKNPDYGTDEAQWWRHLEE
                     SLERLQTDYIDIYNHHGISWERYEEHVLPRVSRWMVKAKEQGLIRHICCSFHDDNEAL
                     KRLVDTGYPEVITVQYNLLDRSLEEGIAYAHEKGVGIVVMGPVGGGRLGAPSEVLSTM
                     IPGINRVPELALRFVLANPHVSVALSGMSTLQQVEENVATASSGATLSEAELAAIDEQ
                     MARLSKMADLYCSGCRYCLPCPNEVNIPVLFELYNMGRVYGLWDVARARYAKVGKIPW
                     LPGKTADACVECGECEEKCPQRIAIPAQLAEAHQALTA"
     gene            4659..5645
                     /locus_tag="PLY56_01780"
     CDS             4659..5645
                     /locus_tag="PLY56_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20239.1"
                     /translation="MQVDGRWLKHTLDFALSGGRLNLEDATALLEIPLGTEESRAIAD
                     AAHALALRQSRQGRIWTAIGVDYCPCPQNCSFCSFGSDWGIVQDSHEWTEEQVVDAAA
                     RFAAEGASWITIRTTQHYPLERVCSLARAMRERIPNCPTLVANVGEMSLEQAEMLYDA
                     GYSVAYHVFRLREGTDTGIDAEERLRSLENIRRSRLGLAFLAEPIGPEHTAAEIAREM
                     VRARDLGAVIMGAMARVPVPGTPKAHLGQIPEEKLARIIAVSRLVGGEVAQEICVHPP
                     SDAAVAAGASAVVVETGAVPRDSEEIRKEWRGLTMAQAKQLLGRHYPSVAGA"
     gene            5661..6665
                     /locus_tag="PLY56_01785"
     CDS             5661..6665
                     /locus_tag="PLY56_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ20240.1"
                     /translation="MDKARWLGVLVAIALVAGLALLTRREARKTTQEVQRVSVGTWKT
                     AQTIAPFGYQRFLEGRYEVEVLPFTNPGDQKTALLAGSLRMCGTTLVHAITSASRGEP
                     VVVVAALCDKCSALVVHKDSEIHDPADLRGKKIGYVPSTMHDLLLRETLTRAGLDPLR
                     DVKLVRIDFFDMGQALSTGGIEAFLSGEPFPTMAKVEGYGRILSYPYFDDSIGTLNAG
                     MLVTRNEIEEDPELVQDLVTAHALATEYYNAYPEEWLRAASEFGTPLEILKNAAENIA
                     LTWKMDEKYLQAARNLGKQMLALGMITQEPDYEQLFDLRFQEKAAQAVAARSGEWRSQ
                     "
     gene            6650..7465
                     /locus_tag="PLY56_01790"
     CDS             6650..7465
                     /locus_tag="PLY56_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ20241.1"
                     /translation="MAQPIAIERPEAAAPRRLDWLPFILPGVLLSAWALLSHTGHVPG
                     YVLPSPGRLVLSALDFVLGKWHLDAYSGTFLQHAAASLQRVALGFALAALAGVLCGLL
                     TGCSPHARRLLDPSVHAVRAVPGIGWLPIAMVWFGVGTRTAVFLIALAAFFPIYVNAA
                     LGARTVRPLWLRAARMLGASQRDLFLTVTLPAGLPATAEGLRLGLGVSWAYLVLGELT
                     GVPDGLGAVMMDARMNGQVEVILTSMLAIAGMGRLSDLLLVALFRRVLPLHPR"
     gene            7479..8300
                     /locus_tag="PLY56_01795"
     CDS             7479..8300
                     /locus_tag="PLY56_01795"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014602921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20242.1"
                     /translation="MDSAFLQSCHIVVHQAGKYYPGHGGGEGLWAVRDLTLCARPGEF
                     AAVVGPSGCGKSTLLSLLAGFEQATEGKVMLEGRPVSGPGPDRGVVFQEDALFPWLTV
                     LGNVCYGLHRRGVSRPEARDEAMRFLHLVGLEEFADYYPSQLSGGMRQRVALARVLAN
                     RPRALLMDEPFGALDALTRMEMQQLLLDVWQKLNVTVLFVTHDVEEAIFLADRVYVMT
                     ARPGRVLRRVEVGLPRPRTPDTYTDPALTRLRHEVLDLLLEARNPSVLSDAIYPY"
     gene            8363..>9121
                     /locus_tag="PLY56_01800"
     CDS             8363..>9121
                     /locus_tag="PLY56_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20243.1"
                     /translation="MQKRLSVCLLLVLALSAGPSWAQVTAPWQPWTWPKKLQLGPVSF
                     YAAQRVSLIDDEKANSTYFRNYDLLLYTDWRMSEKEQLRVYALNNPVHKSHPQVKSPW
                     WLIYAYYERDLGSGLLRVGKVPFPFNYQNGFPEWHSQVRRVTRVWDYGMTWSSKPKDG
                     WQWDIGWVTDGTTTSSDIDRMNEPAFVGRIRAGVGERLEAGVSGMYAPKRTIAGTEAD
                     LSRFGAHLRWLPSSRLELRGEYVDFRTLSQGSVIR"
BASE COUNT         1533 a         2895 c         2991 g         1692 t
ORIGIN      
        1 gcggccagta tggcgggcat ggagcgaggg gccaggcagc ggtgaactcc tcgcatcggg
       61 caggagtgct tcctgagtag tcgcatgggt ggatccctcc tttcgaattg gatgattcag
      121 gcgagcgcct ttgttcctgg ggagaggggc caaaggggtc cggcactccc gagggtgggg
      181 tgccgaaccc ctggcgaatc tgcgaactgc agtacggggc gaagcgcgcc cggcggccag
      241 gtgaagtggc cgataggggc gcccggtcgc cgccctaccg gcgggcaggc gcgttgctcg
      301 ccggttgccc gggcgcctcg gccggcgcgt tgccggccgg ctgcgcgggc gcgttggccg
      361 gcgccttcgc cggtggcttc agatcgtccg gaggcgttgg ccgcggcttg atgcccttct
      421 gtttcaggct cgcgatggcc gcatccaggc cgttgaagcg gttcttcgga tcccacatca
      481 gaaactccgg gatcccccgg tcgaaggtgg ccttgatctg cgcggcgatc tggggtgggc
      541 catagtgaga cccaccatag cggccgcggt ccggcaggct aaagttctgg agccacggcc
      601 ggatcttgca cccggtccct gccagtcgcc tctgcgcgtc gcgcaggctg acatagacga
      661 tgtcataggg cgcggcgtcg gggttgggaa tcccgtactc gcccttgtga tagtgcgacg
      721 ggtagaccat cgggcagatg tagtccacgt tctcagcaat ctggtggatc agctggccga
      781 tgcccatgtc gtcgctcacc aggccggtca gcccaaagat atcagcggag acgatggcgc
      841 catagggctc cagcttcttg cgcgtgtagg ccaggaactg ggcgatcacc tcgttcggcg
      901 gggtatccgt cttggcaggg aagcggaggt tgcccaactt cccatccgtt gggaaccgaa
      961 cataatccca ctggatctct ttgaagccct tcttcatcgc ctcgatggcg acggccgcgt
     1021 tgtagtccca accctccttg ttgtagggat cgagccaggc ctccttcttg cggttctccc
     1081 agatgccgcc gctggcgttc ttagcggcca tatccgggcg cacgcgcggc aggatatcat
     1141 cggcgaagac ggcaatgcgg gcgatggggt agatgttgtt ctccttcagg aggttcatca
     1201 cccagtcgat gtcggcgcag cgctgcgcat agccgtagtg gttgatctcc ttgatctggc
     1261 cgcgcttttc ctcgacctgc ttcgcccacg ggatatccat cttgaaggtg atggcgccgt
     1321 ccgaatcctt cacgtcgatc acgacggcgt tcatgccggt ggctttgagc agctccacga
     1381 tcttttgcat cttctgcttg ccgccggcac tccacccggt gaggtagacg cctcgcgcct
     1441 cgatgaactc cttcttcggt ttgggcggct ccatcgnnnn nnnnnnatcg actgctgctg
     1501 tgaggccggg ccattggccg gcggcgcccc cggccccgtg ctgctgcggc agccagcgac
     1561 ggctacggcg gccaggacgg cggtcaggat gacgtactgc acgttcttca acttggcctc
     1621 ctcggaacga aactgagagc gaaccgatgc cggaccacac gggcccaaca acagacaaac
     1681 cggcaggaat tgtgccatgc cggtggaact agacagcaac ctatttttct gctgtcctgc
     1741 gagcgatcct gcgtgcgcac tatgcaggca gctgtcgtgc ccgaaaagga tgcacgcaaa
     1801 ctattatagc ataaacaatt tggcctgtgt agtgcgttcg ctgagtggga gggatgggtt
     1861 ggcaggtgat cagccagagg gggtgcgcag atgccatatc gccgtggcgc gccgcctctt
     1921 gtgttgtaca caccgattat tggggaaatg tccctggtta tcgctggatc gcaggttcac
     1981 tgctgcctct cgcgctggag tgccccggct actcgttcca gctggtggga ggcagacgtt
     2041 ctcggagagg aaacgaatgg catcacgtcg cagcatccta tcctcgatct tgggcatctc
     2101 cttggccgcg ctcctgttgg gggccggctg ccagcggcag agttcgacta ccgcgcctgg
     2161 gcccgaacca gggcagaagc cggcacaaca gagtgccgcg ccggcgccag cacccccggt
     2221 gagcgacgcg gcgcccaacg aattggggcg cgtgccggtg ctcgaatacc acgtcatcgg
     2281 gcccaaagaa ggccgctggg cgcgcacccc ggaggcgttt cgcaaagacc tggaaacgct
     2341 ctacgcgcac ggctttcgcg ccgtctcgct gcgcgactac gtgaccggca atatcaacct
     2401 gccccgcggc gccagcccgg tggtcttcac cttcgatgac tccacggaag gccagtttcg
     2461 ctacctggag aaggatggcc agctcgtcat cgatcccgac tgcgccgtcg ggattctaga
     2521 agagttcaac cgcaagcatc ctgacttcgg tttcaaggcc accttctacg tgctccccga
     2581 gtctgcgttc ggccagccgc aataccgcaa gcagaagctg cagtacctgg ccgagaaggg
     2641 cctcgagatt ggcaaccaca cctggagtca caccgctctg aagcggcttt ctaacgagaa
     2701 ggtgcaggaa gagctggcac ggccgcagca ggcgattggc gaggtggtgc ctaacttccg
     2761 cttcttctcg ctggcgctcc cgctaggcat ctcgccgaag gagaaggcgc ttgcgagcaa
     2821 gggcgcctgg aatggcatca cctacgagca tcacgccgtg ctcctggtcg gctccgtgcc
     2881 ggcaccctcg ccctatgaca aggacttcga tccgcgcgcc atccagcgcg tgcaggcgac
     2941 agactggccg gaggtcaagc cattccagct cagcgcgcag atcaagacgc tggtggagac
     3001 ggaaaaccgc cgctatgtga gcgacggcga cccgaatacg attgccgtgc cggcggctct
     3061 gaaggatgag ctggcgcctg agaggacgca gggcaaggaa gtgaagatct actgagcgga
     3121 aagctacccc ggctcccgga tggatgcgga ggggcctcga gcgcccctcc gcgcccttcc
     3181 gggaagcgcg agagaatcgc gctcaagctg tgagcgcctg gtgtgcttca gcgagttgcg
     3241 cggggatggc gatccgctgc gggcatttct cctcgcactc gccacactcc acgcatgcgt
     3301 ccgcggtttt gcccggtagc cacggaatct tccccacctt cgcgtagcgc gcgcgggcga
     3361 catcccacag gccatacacc cgccccatgt tgtagagttc gaagagaacc ggaatattca
     3421 cctcgttggg gcaggggagg cagtagcggc acccggagca gtagagatcc gccatcttac
     3481 tcagccgggc catctgctcg tcgatggcgg ctagctccgc ctcggagagc gtggcgccgc
     3541 tggacgcagt ggcgacgttc tcttccacct gctgcagtgt gctcatcccc gagagggcca
     3601 cactgacgtg ggggtttgcc agaacaaagc gcaaggcaag ctctggcacg cggttgatgc
     3661 ccgggatcat cgtgctgagc acctcgctcg gggcccccag gcgcccgcct cccacgggcc
     3721 ccatcaccac gatgccgaca cccttttcgt gcgcgtaggc gatcccctct tccaggctgc
     3781 ggtccagcag gttgtactgc accgtgatca cctcgggata gccagtgtct accaggcgct
     3841 tgagcgcctc gttgtcgtcg tggaaggagc agcagatgtg ccggatcagg ccctgttcct
     3901 tcgctttcac catccaccgg ctcactcggg ggagaacgtg ctcctcatag cgctcccagg
     3961 agatgccgtg gtggttgtag atatcgatat aatccgtctg caggcgctct aggctctcct
     4021 cgaggtggcg ccaccactgc gcctcatcgg tgccgtagtc cgggtttttc gtcgaaacga
     4081 tgatccggtc gcgccatccc ttgagcgcgt cgcctacggc gcgctggcta tcgctgttgc
     4141 aatagccgac ggcggtatcc acgtagttga cgcccgcctc gaatgcccga tgaagcatcg
     4201 gcacggcaag ctcgcggtca actgtcccct cggtcatccc gcccttcatc ggcaggcgca
     4261 tacatccgaa gcccagctgc gatactctca agccggttcg gcctagcatc cgatagatca
     4321 aagcaatccc tcccggagat aggtttcggc acctgttggg ctcgactcct ggcggctccg
     4381 cctgaagagg cccggtgggg cgaccggatc cttgatccgc aaatgacgcg cctatgggcc
     4441 acgcggggct gccgccgcaa gggccgggca tggccggtag cacatccccc agagaaccag
     4501 gtgctgcccc cagacacggg gaattgacag atgcacggtg tcgtgatacg atatgacacg
     4561 ctttgccatg cctcgatctg ggttgccaag aacgttccaa gcaaggcaga gagcctctgg
     4621 tctgtgatgc aagcaaagcg atccgaaggg acaccgggat gcaagtggat ggaagatggc
     4681 tgaagcacac cctcgacttt gcgttgagcg gtggccgcct gaacctcgaa gacgcgaccg
     4741 cgctgctgga gatccccctc ggcaccgagg agtcccgtgc catagcggat gctgcccacg
     4801 cgctggcact tcggcaatcg cgccaggggc gtatctggac ggcgattggc gtggactact
     4861 gcccctgccc gcaaaactgc agcttctgct cctttggatc cgactggggg atcgtccagg
     4921 actcgcatga gtggaccgag gagcaggtcg tggatgccgc ggcgcgcttt gccgcggagg
     4981 gcgcgtcctg gatcaccatt cgaaccaccc agcactaccc cctggagcgc gtgtgcagcc
     5041 tggcgagggc gatgcgcgag cgcattccga actgccccac gctggtggcg aacgtcggcg
     5101 agatgtcgtt ggagcaggcg gagatgctct acgacgccgg gtacagcgtg gcctatcatg
     5161 tctttcggtt gcgcgagggc accgatacgg gcatcgacgc tgaagagcgc ctgcggagcc
     5221 tggagaacat ccgccggtcg cggctaggtc tggcgttcct agccgagccg attggtccgg
     5281 agcacacggc ggccgagatt gcccgcgaga tggtgcgcgc gcgcgacctg ggcgctgtga
     5341 tcatgggcgc gatggcgcgc gtgccggttc ctggcacccc caaggcgcac ctcgggcaga
     5401 tcccagagga gaaattggcg cgcatcatcg cggtcagccg cctggtcggt ggcgaagtcg
     5461 cccaggagat ctgcgtccac ccgccctcgg atgctgccgt tgccgcgggc gccagcgccg
     5521 ttgtggtcga aacgggtgcc gtgcctcgcg acagtgagga gatccgcaag gagtggcgcg
     5581 ggctcactat ggcccaggcg aaacagctgc tcggtcggca ctatccctct gtggccggcg
     5641 cctgacacga ggaggttcca atggataagg cccgctggct gggtgttctc gtggcaatcg
     5701 cgctcgttgc cggcctcgcg ctgctgaccc gccgggaagc tcgaaaaacg acccaagagg
     5761 tgcagcgcgt cagcgtgggc acctggaaga cggcgcagac gatcgcgcct tttggctacc
     5821 agcgtttcct ggaaggccgg tatgaggtgg aggtgctccc cttcaccaat ccaggagacc
     5881 agaagaccgc gttgttggcc gggtcgctgc ggatgtgcgg gaccaccctg gtacacgcga
     5941 tcacctcggc gtcgcgcggc gagccggtcg tcgtcgtggc cgcgctgtgc gacaagtgca
     6001 gcgccctggt ggtccataag gactccgaga tccacgatcc agccgacctg cgtggcaaga
     6061 agatcgggta tgtgccctcg acgatgcatg acctcctgtt gcgcgagacg ctcacgcgcg
     6121 ccggcctcga cccgctgcgc gacgtgaaac tggtgcggat cgacttcttc gatatgggcc
     6181 aggcgctctc gaccgggggg atcgaagcct tcctcagtgg cgagccgttc ccgacgatgg
     6241 cgaaggtgga gggttacggc cggatcctca gctatcccta cttcgacgac tctatcggta
     6301 cgctcaacgc cggcatgctc gtcacgcgaa acgagattga ggaggatccg gagctggtcc
     6361 aggacctggt gacggcgcac gcgctcgcca cggagtatta caatgcatac cccgaggagt
     6421 ggctccgcgc cgcgtctgag ttcggtaccc cactggagat cctgaagaat gccgcggaga
     6481 acatcgcgct tacctggaag atggatgaga agtaccttca ggccgcgcgc aacctcggga
     6541 agcagatgct ggcgctgggg atgatcaccc aggagccgga ctatgagcag cttttcgacc
     6601 tgcgcttcca ggagaaagcc gcccaggcgg tggccgcccg gagtggagaa tggcgcagcc
     6661 aatagccatc gagagaccgg aagcggcggc accgcgccgc ctcgactggc tgccgttcat
     6721 ccttcctggg gtgctcctct cggcgtgggc actcctctcc catacaggcc atgtgcctgg
     6781 atatgtgctc ccttcgccgg gccggttggt gctcagcgcc ctcgacttcg tgcttgggaa
     6841 gtggcacctc gacgcgtata gcggcacctt cctgcagcac gcggcagcca gtctccagcg
     6901 cgtcgcgctc gggttcgctc tggcggcgct cgccggcgtc ctctgcggtc tgctgactgg
     6961 gtgcagcccg catgcccggc gcctgctgga cccctcggtc cacgctgtgc gcgccgtgcc
     7021 gggtatcggg tggctaccga ttgcgatggt ctggttcggt gtgggcacgc gtacggcggt
     7081 cttcctgatc gcgctggctg ccttctttcc gatctatgtg aacgccgccc tgggagcgcg
     7141 caccgtccgg cccctctggc tacgagccgc gaggatgctt ggggcatcgc agcgcgacct
     7201 cttcctgacg gtcacgctgc ccgccgggct tccggcaacg gcggagggac tgaggctggg
     7261 gttgggcgtc tcctgggcct acctggtcct aggcgagttg acgggcgtgc cggatggcct
     7321 tggcgccgta atgatggatg cccgcatgaa tgggcaggtg gaggtgattc tgacctcaat
     7381 gctcgcgatt gcgggcatgg gccggctgag tgatctcctt ctcgttgccc tcttccggcg
     7441 ggtgctgccg ctgcacccgc gctagagggg gaacaccgat ggactcagcg tttctacaga
     7501 gctgccatat tgtggtacac caagcgggca agtattaccc gggccatgga gggggcgagg
     7561 gcctctgggc tgtgcgcgac ctgacgctct gcgcgcgtcc tggcgagttt gccgcggtcg
     7621 ttggccccag cgggtgcggc aaatccacgc ttctctcgct gctcgcgggc ttcgagcagg
     7681 ctaccgaggg aaaggtgatg ctggaaggcc ggccggtttc cggtccgggc cccgaccgcg
     7741 gggtcgtctt ccaggaagat gcgctctttc cctggctgac cgtcctcgga aacgtctgct
     7801 acgggctgca tcggcggggc gtctcccgcc cggaggcgcg cgatgaggcg atgcgcttcc
     7861 tccacctggt cggcctggaa gagttcgccg attactaccc gtcgcagctc tcgggtggga
     7921 tgcgccagcg cgttgccctg gcgcgggtac tcgccaaccg gccgcgcgct ctcctgatgg
     7981 atgagccgtt tggcgctctc gatgcgctca cccgcatgga gatgcagcag ctcctgctgg
     8041 atgtgtggca gaagctcaac gtcaccgtcc tcttcgtcac gcatgatgtc gaagaggcga
     8101 tctttttggc tgaccgggtc tacgtcatga cggcgcgccc tgggcgcgtg ctccggcgtg
     8161 tcgaggtggg gttgccccga ccgcgcaccc cggataccta caccgacccg gcgctcaccc
     8221 ggctccggca cgaggtactc gacttgctcc tcgaagcgcg taatccttcc gttctgtcgg
     8281 atgccatcta cccctactga ggttccactg gaatcgcgcc cttatcgggc aaatggacaa
     8341 aacagccaat cggagacaaa ctatgcaaaa acgactcagt gtctgcctgc tcctcgtgct
     8401 ggcgctcagc gccggcccct cctgggcaca ggtgaccgcc ccgtggcagc cctggacctg
     8461 gcccaagaag ttgcagctcg gcccggtcag cttctatgcc gcgcagcgcg tcagcctgat
     8521 cgacgatgag aaagcgaact ccacctactt tcggaactac gacctgttgc tctataccga
     8581 ttggaggatg agcgagaagg agcaactccg ggtttatgct ctcaacaacc cggtgcacaa
     8641 gagccatccc caggtgaagt cgccgtggtg gctgatctat gcctactacg agcgcgacct
     8701 gggcagcggc ttgctgcgag tggggaaggt gcccttcccg ttcaactatc agaacggctt
     8761 cccggagtgg cacagccagg ttcgccgggt gacgcgagtt tgggattacg gcatgacttg
     8821 gtcgagcaaa ccaaaggacg ggtggcaatg ggatatcggg tgggtcaccg atggaacgac
     8881 cacctcgtcg gatatcgacc ggatgaatga gccggcgttt gtaggccgga tccgggccgg
     8941 cgtcggcgag cgcctggaag ccggtgtctc ggggatgtac gccccgaagc gcaccatcgc
     9001 cggcacggaa gcggatcttt cgcgctttgg cgcgcacctg cgctggctgc cctccagccg
     9061 cctggagcta cgcggcgaat atgtggactt ccgcaccttg agccagggca gtgtgatccg
     9121 c
//
LOCUS       DAOSCV010000038         8868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5154_length_8868_cov_3.521729, whole genome shotgun sequence.
ACCESSION   DAOSCV010000038 DAOSCV010000000
VERSION     DAOSCV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5154_length_8868_cov_3.521729"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLY56_01805"
     CDS             complement(<1..809)
                     /locus_tag="PLY56_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ20244.1"
                     /translation="MAETAKFLAIDLGAESGRGVVGLFDGSKLRLEEVHRFPNGPVRV
                     LDSLHWDALRLFSEMKAALAKCVQEHGSDLQSVGVDTWGVDFGLLGKGDVLLGNPYHY
                     RDKRTDGMLEEAFRRVPREEIFERTGIQFMKLNTLYQLLAMRVEGSPLLDMAERLLFM
                     PDLFHFFLTGNKVTEFSIATTSQMYDPRARGWATGMLQAMEIPTGILGEVVPPGTEVG
                     TLLPSIGAETGGAGVPVITPAAHDTGSAVAAVPARGADWCYISSGTWSLMG"
     gene            complement(1357..2541)
                     /locus_tag="PLY56_01810"
     CDS             complement(1357..2541)
                     /locus_tag="PLY56_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ20245.1"
                     /translation="MRIIHNVTVLPVSGPAIREGAVALEAGRIVAVGRAPGGDFDSLG
                     LGRAAASATLIDGEGGVLTPGLIDAHTHLGVSEEAIGPAGDDVNETTSPVTPDLRAID
                     GINPEETALPRALRAGVTAVCVLPGSANVIGGSGAVIRTHGTVVDRMLLRENAGMKAA
                     FGENPKRFYGSKDKAPATRMAIAALYREWLTKARDYDARKARAREKGEPVDTDVKLEA
                     LLPVVRGEIPLRAHAHRADDIATAIRIAREFGLRLVIEHCTEGHKVVDLLLEHGIPAV
                     VGPSMGARGKVELREKGFETAGVLARAGVLVALTTDHGVMHIEHLQVAAALAVKAGMT
                     EEQALAAVTLSPARILGLEERLGSIDRGKEADLVLWSGDPLDVRSYPRRVWVAGEEVV
                     TA"
     gene            complement(2599..3024)
                     /locus_tag="PLY56_01815"
     CDS             complement(2599..3024)
                     /locus_tag="PLY56_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20246.1"
                     /translation="MWYTNGTTTYTTYGNYQPLVYPYVKNKEVFFCPSSNNHNNDRSL
                     RFAYDYAMNSRIGTVTPPRSMSQIDSPAEIFLCADSNYEWIDRAARIDARHTAGANLV
                     FCDGHVKWMRGSAIAASPQLCWPDFSKDTWLVSGPPPEK"
     gene            complement(3087..3239)
                     /locus_tag="PLY56_01820"
                     /pseudo
     CDS             complement(3087..3239)
                     /locus_tag="PLY56_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            3553..7458
                     /locus_tag="PLY56_01825"
     CDS             3553..7458
                     /locus_tag="PLY56_01825"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOJ20247.1"
                     /translation="MIQAAPVCAIVAGLLLLAGIIPAGAAPEGNLLRNPSVEELEAGQ
                     ERPAAWVANHFNTGGTWAVGTDGGHDGGRYLILRSATETQRACWRQKVALPEGAKAVA
                     FGGHYRTRGVAESGQGGASLRVLFHRDMAKWDEIGLKQAFFPPAAEWTEAQGLFFVPE
                     GTRAVVLEAFHWLTPGETHWDSLWLRVATEEDLKNVPFEPGTEIDREPVPGRNKPYSP
                     ADGETVRLNPPPFLWLPSGREVTYRLQIARNDRFEGRDVISLEGLEWCAEMLTRPLEP
                     GIWYWRYGVDRPGLPTAWSRARRFEVARDASPWVYPGRDAFTVPTARPRLFVHAGRLA
                     ELRERARSGDLKSIADALVRSIQRHIGEPLVPEPKHLTGTGQTRSAQYLEVFVTTRPP
                     MDAMERAALAYLLTGDRACGDEAKRRILHFFSWNPKGSTSVSHNDEPAMWIMMRGVRA
                     YDWTYDLFTPEEQEKIEAVMRVRAADFYEHLRRRPFENNPYESHSGRIIGFLGEAALA
                     FLPEWPEARKWLDYVTKIYWGVYPAWGKDDGGWNEGPGYWSAYMTFALHFVVALREAT
                     GIDLSQRPFFRSTPYYLLYLTPPYSNMSPFGDGTQFRPSQPAGLMYQFSSLLRDPAIR
                     WYSDALGRGASSDILGIVLKDDSLKGMPPVHLPQARHFEGVGLVCLHTQLINGEENVL
                     FAMRSSPYGAVSHGHNDQNTFVLEAFGEPLAIASGYYPRYGSPHHDQWTRQTKAKCGI
                     TFDGGQGQDRGWHAKGAITAFVHGEGFDFVSGDATPAYGGRLTRAVREVIHVRPGIIV
                     IRDDLASAEPRRYEYWLHALDEMKVDAAARTVTTTRPKATLRTRFLHPEGLVFTQDNK
                     YEPAPEGRPDRTSYSSTWYVTATTREPAQATEFLSVLLPAKAGQEANLPTTRHLESKT
                     VRGVELRFPDGSRTVAGFALPGVTGTVSLEEISTDGRTFAVAYDANGQPRSWLLSGGR
                     TLRDGQRVLAQAAEPVTLTAAVTEDGGRIDAAGPGGEATLWWPKPPRALTRDAETVRF
                     SQARQGAIRLSLNPREDRVEVWAREPVPPGEAKLLLETNGQVVELTGRRFGRGRAAVS
                     GRVNVDPGAYRFDVPKGVDVTAGVADAQGRFWLRGDEMVMLSGAALPDRVKLVAALKA
                     ARLSAQPQSRLPQGITYEAEQNWRVTGGEVEVSRGGHANVSGEANLWSWNTPGSTISW
                     EIEVKEAGTYEIWFVGATEGGLLAEFAVDGGEALGLRFEPTGGWGRTAAGEWRAFQVR
                     AASGTPARFKLDAGRHRLALTNRTGVGLNLDRILLMPVR"
     gene            7790..>8868
                     /locus_tag="PLY56_01830"
     CDS             7790..>8868
                     /locus_tag="PLY56_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ20248.1"
                     /translation="MTCPRYSFPWRLATGLVAALLLLVAAPSAWSAEEEQNLVTNPGF
                     EEGNTGWNLPQTYTVVDDVAHSGKRSLRVVNTDPDRYLLASQPLKLKPGMMYRFSAWV
                     RAQGVQGNDTGATICVEWYGEKGFIGGTYPGGIKGDRDWYRIEAVTPPIPEAATRCQV
                     TLYLRKKMTGTAWFDDVSVVAVYPPALDAALVFPNYRGILWPDSDKQQVVLRGQVGAY
                     LKGGLRPEQMTLAGSLLDGEKVIDERRIRQVGAGMHEVTLNARGLRPGNYIARMELLA
                     PDGKPLERKEFAVRKLAPGTPRPKVYIDEHNRTIVDGKPFFPLGWYFGPGPGNEGFQE
                     HIDRVAESPFNTIMCYGINYGGQEKV"
BASE COUNT         1514 a         2946 c         2941 g         1467 t
ORIGIN      
        1 atccccatca gcgaccacgt gcccgatgag atgtagcacc aatcggcgcc gcgcgcgggc
       61 accgcggcga ccgccgagcc ggtgtcgtgc gcagcggggg taatcaccgg cacgccggcg
      121 ccacccgtct ccgcgccgat ggagggaagc agcgtaccga cctctgtgcc cggcggcacc
      181 acctcgccca ggattcccgt cggaatctcc atcgcctgca gcattcccgt ggcccacccg
      241 cgcgcacgag gatcatacat ctgcgaagtg gtggcgatgc tgaactcggt caccttgttg
      301 ccggtgagga agaagtggaa aaggtccggc ataaagagga ggcgctcggc catgtcgagg
      361 agcggagagc cctcaacccg catcgctaga agctggtaga gggtattcag cttcatgaac
      421 tgaatgccgg tgcgctcgaa gatctcctcg cgcggcacgc gccggaacgc ctcttccaac
      481 atgccgtcgg tgcgcttgtc gcggtagtgg taggggttcc ccaggagcac gtcacccttc
      541 cccaggaggc cgaagtcgac gccccaggtg tcgacgccca ccgactggag gtcggaaccg
      601 tgctcctgca cgcacttggc gagtgctgcc ttcatctccg agaagaggcg cagcgcgtcc
      661 caatggaggc tatcgagcac gcggaccgga ccattgggaa agcggtgaac ctcctccagg
      721 cgcagtttgc taccatcaaa gaggccgaca accccgcgcc cgctctcggc gcccagatca
      781 atcgccagaa acttcgctgt ctctgccatc gcacgttccc ccccgtggta tccgcgtggc
      841 gccgtccccg cctggagact acgccctcgc tctgcatgcg aacacccgat cacttcttcc
      901 gggaatggcg cttctcctgc cgagaggtgg ggacgcgggc gccaacgccc gggccagatg
      961 taaggggagc caccgcgcgg cggtcgaggg catcgcgcgg cgataaatca ccgcgctgct
     1021 tgtgagcgaa gccctgccgg gctcggctgg gcaacacttc ccggcacctt agaacggcag
     1081 gggatcggag agggagcccc gaagaggcct tgccaaaaag cagcgcggag cttcgagctc
     1141 cgcgcgccag gatatgcata gccccacacc cgcaacgcgg cgataaatca ccgcgctgct
     1201 tgtgagcgaa gccctgccgg gctcggctgg gcaacacttc ccggcacctt agaacggcag
     1261 gggatgggag ggggagcccc gaaggggctt tgccaaaaag cagcgcggag cttcgagctc
     1321 cgcgcgccag gatacatatc gcccgtgctg ggtcggtcag gcagtgacga cctcttcgcc
     1381 ggccacccaa acacgccgtg gataggagcg cacgtcgagg ggatcgccag accacaggac
     1441 taggtcggcc tccttgccgc ggtcgatgct ccccaggcgc tcttccaggc ccaggatgcg
     1501 cgccggggaa agcgtgaccg ccgcgagcgc ctgctcctcg gtcatgcccg ccttcaccgc
     1561 cagcgccgcg gctacctgga ggtgctcgat gtgcatcacg ccgtgatccg tggtcagcgc
     1621 caccagaacg ccggcgcgtg ccagcacgcc cgctgtctcg aagcccttct cgcgcagctc
     1681 caccttgccg cgcgcgccca tactcgggcc aaccaccgcc gggatgccat gctccaggag
     1741 gagatcgacc accttgtgcc cctcggtgca gtgctcgatc accaagcgca ggccaaactc
     1801 gcgcgcgatc cggatcgctg tcgcgatatc atcggcgcga tgggcgtgcg cgcgcagcgg
     1861 gatctcgccg cggacgaccg ggagcagcgc ctccagcttc acatcggtat cgaccggttc
     1921 gcctttttcg cgcgcccggg ctttccgggc gtcgtagtcg cgcgcttttg tcagccactc
     1981 gcgatagagc gccgcaatcg ccatgcgcgt cgccggcgcc ttatccttac tgccgtagaa
     2041 cctcttcggg ttctcgccaa aggcagcctt cattccggcg ttctcgcgca agagcatgcg
     2101 gtcgaccacg gtgccatgcg tacggatcac cgcgccactg cctccgatga cgttcgcgct
     2161 gccggggaga acgcaaacag cagtgacgcc ggcgcgcaac gcccggggga gcgccgtctc
     2221 ctccgggttg atcccgtcaa tcgcgcgcag gtcaggcgtt acggggctgg tcgtctcgtt
     2281 gacatcgtcg ccggcgggcc caatggcctc ttcgctcact cccaggtgcg tgtgcgcatc
     2341 gatcagcccc ggagtgagca ctcccccttc gccatcaatg agcgtggcgc tggcggcggc
     2401 ccggcccagc ccgagggagt cgaagtcgcc cccgggcgcc cggccaacgg ccacgattct
     2461 ccctgcctcg agggcaaccg cgccctcgcg aatcgccggt ccagaaaccg gcagcaccgt
     2521 gacgttgtgg ataatccgca ttaaacgccc tccctcggcc cgcgcccttc tgccgagatc
     2581 ccggcagcta atctgcggct acttctccgg cggcgggccg ctgaccagcc acgtatcctt
     2641 cgagaagtcc ggccagcaga gttgcggact ggccgcgatg gcagagccgc gcatccattt
     2701 cacgtgcccg tcacagaaga ccaggtttgc gcctgccgtg tgccgggcat cgatccgcgc
     2761 cgcgcggtca atccattcgt agttagagtc ggcacacagg aagatctcgg caggcgaatc
     2821 gatttgagac atgctcctgg gtggcgtcac tgtgccgatc cgggagttca tcgcgtagtc
     2881 gtaggcaaag cgcagcgatc gatcattgtt gtggttgttg gacgaagggc agaagaaaac
     2941 ctccttgttc ttgacgtacg ggtagacgag tggctggtag ttgccatagg tggtataggt
     3001 cgtcgtgccg ttggtatacc acaccggtcc atcgaccccg ttgcgctcgg tggggaacgt
     3061 ctcgtcatag tcacgtacat catcagcccg gtgccgatct gctttagatt cgactggcag
     3121 gatgattttc tcgcgttctc gcgcgcccgc gcgaagacgg gaaacaggat ggccgccagg
     3181 atggcaataa tggcgatgac gacgagcaac tcgatgagcg tgaagccttg tttcctcatg
     3241 gtacgtactc cttctgccgg gcaccctgcg tctccggcac cgaacgcccc ggggcagccc
     3301 ggctccatcg ccttttctac gccgggctgg tgctctcctc cctaagttag ttgaatgaac
     3361 tagttaagga agataatgct ctgccagctc tcgtcggttc acctgtcgag cacgctggac
     3421 gccccctccc aggcggagag gtggtggcga agtatgctcc ggcgcaggag ccggccctta
     3481 gatgtctaat ccagtggcaa gcgagagcgc gagggcaccc cggtttcgcg gtctcgaagg
     3541 agggcgtgac tcatgatcca agccgcacct gtttgcgcaa tagtggcggg cctgcttctc
     3601 ttggcgggga tcatcccggc gggagcagcc cccgagggaa acctcctgcg aaacccgagc
     3661 gttgaggagc tcgaggccgg gcaagagcgc ccggcggcat gggttgccaa ccacttcaac
     3721 acgggcggca cctgggccgt gggcactgat ggaggccatg acggggggcg ctacttgatc
     3781 ctccgctcag ctaccgagac gcagcgcgcc tgctggcggc agaaagtcgc tttgcccgaa
     3841 ggcgcgaaag cggtcgcgtt cggtgggcac taccgcacac gcggggtagc cgaatccggg
     3901 cagggcgggg catccctccg cgttctcttc caccgcgaca tggcgaaatg ggatgagatc
     3961 gggttgaagc aggcgttctt cccgccggcc gcggagtgga ccgaggcaca gggtctcttc
     4021 ttcgtgccgg agggcacgcg cgccgttgtc ctcgaggcat tccactggct tacccccggg
     4081 gagacgcact gggatagcct ctggcttcgc gtcgctacgg aagaggatct caagaacgtg
     4141 ccattcgagc cgggcaccga gatcgaccgc gagccggtgc cggggcgcaa caagccatac
     4201 agccccgccg atggcgagac ggtgcgtctc aacccgccgc ctttcctctg gctcccctcc
     4261 ggacgcgagg tgacctaccg cctgcagatc gcgcgcaacg accggttcga gggccgcgac
     4321 gtgatctccc tggagggact ggagtggtgc gccgagatgc tcacgcgccc gctggagccc
     4381 ggcatctggt actggcgcta tggcgtggac cggcctgggc tgcccacggc gtggagccgg
     4441 gcgcggcgct tcgaggtggc ccgggatgcc tctccctggg tctatcccgg gcgcgatgcc
     4501 ttcaccgtgc ccaccgcccg cccacgtctc ttcgtgcatg ccgggcggct ggcggagttg
     4561 cgcgagcgcg ccagaagcgg cgacctgaag agcatcgccg acgcactagt tcgctccatc
     4621 cagcggcaca tcggcgagcc gctagttccc gagccgaagc acctcaccgg aaccggccag
     4681 acgcgcagcg cccaatacct ggaagtgttc gtcaccaccc gacccccgat ggacgcaatg
     4741 gagcgcgccg cgctggctta cctgctcacg ggcgaccgcg cctgtggcga cgaggcgaag
     4801 cgccgcattc tgcatttctt ctcatggaac cccaagggga gcaccagcgt ctcgcacaac
     4861 gacgagccgg cgatgtggat catgatgcgg ggcgtgcgcg cctacgactg gacctacgat
     4921 ctcttcacgc ccgaggagca ggagaagatc gaggcggtga tgcgtgtgcg cgctgccgac
     4981 ttctacgagc acctgcgccg gcgccccttc gagaacaacc cctacgaaag ccactccggg
     5041 cggatcatcg gcttccttgg cgaagctgcg cttgcgttcc tgcccgagtg gcccgaggcc
     5101 cggaagtggc tcgattacgt cacgaagatc tattggggcg tctatcccgc atgggggaag
     5161 gatgacggcg gctggaacga gggcccgggc tactggtccg cctacatgac cttcgcgctc
     5221 cactttgtcg tcgctttgcg cgaggcgacc gggatcgatc tctcgcagcg gccgttcttc
     5281 cgctccacac cctactacct gctctacctg actccaccct acagcaacat gtcgcccttt
     5341 ggcgatggca cccagttccg gcccagccag cctgccggac tgatgtacca gttcagctcg
     5401 ctcctgcgcg accccgccat ccgctggtac tcggatgcgc ttggccgggg cgccagttcg
     5461 gacatcctgg gcatcgtgct caaggacgat tcgctgaagg ggatgccccc agttcacctg
     5521 ccgcaggcgc ggcatttcga gggcgtcggg ctcgtctgtc tccatacgca gctgatcaac
     5581 ggcgaagaga acgtgctgtt cgccatgcgg tcgtcgcctt atggcgccgt gtcgcacggg
     5641 cataacgatc agaacacctt cgtcctcgaa gcgttcggag agccgctggc gatcgcgagt
     5701 ggctactacc cgcgctacgg ctcgccgcac cacgaccagt ggacgcgcca gacgaaagcc
     5761 aagtgcggga tcacgttcga tggcggccag ggccaggatc gcgggtggca cgcgaagggt
     5821 gcgatcaccg cgttcgtgca tggggaaggc tttgatttcg tctcgggcga tgccactccc
     5881 gcctacggcg gccggcttac ccgagccgtg cgcgaggtga tccacgtgcg tcccggcatc
     5941 atcgtgatcc gcgacgacct ggcgagcgcc gaaccccgcc gctacgagta ctggctgcac
     6001 gccctcgatg agatgaaggt agacgcggcg gcgcgcaccg tcaccacgac ccgcccgaag
     6061 gccacgctgc gcacgcgctt cctgcaccca gaagggctcg tcttcaccca agacaacaag
     6121 tatgagcccg cgccggaagg ccgcccggat cgcacctcct actcgagcac ctggtatgtc
     6181 accgcgacca cgcgagagcc cgcgcaggcg acggagtttc tgagcgtgct cctgccggcg
     6241 aaggcgggcc aggaggccaa cctgcctacc acgcgccacc tggaatcgaa gacggtgcgg
     6301 ggcgtggaac tccgcttccc cgacggctcg cgcacggtcg ccggcttcgc gctcccaggc
     6361 gtgaccggca cggtgagcct ggaggagatc tccaccgacg ggcggacctt cgcggtggcc
     6421 tacgacgcga acggtcagcc ccgctcctgg ctgctttccg gcggacgaac gctgcgcgac
     6481 gggcaaaggg tgctggcgca ggctgcggag ccggtgacgc tcacagcggc ggtcacggaa
     6541 gacggcgggc ggatcgatgc ggccgggcca ggcggcgaag cgacgctctg gtggccgaaa
     6601 ccgcctcggg cgcttacccg cgatgccgag acggtccggt tctcccaggc gcggcaagga
     6661 gcgatccggc tctcgctgaa cccgcgtgaa gaccgggtgg aggtgtgggc acgcgagccg
     6721 gtcccgcccg gtgaggcaaa actcctcctg gaaaccaatg gtcaggtggt cgagctcacg
     6781 ggcaggcggt ttggacgcgg gcgcgccgcc gtgagcggcc gggtgaatgt cgacccgggc
     6841 gcttaccggt tcgacgttcc gaagggcgtg gatgtaaccg cgggcgtggc agacgcgcag
     6901 gggcgcttct ggctccgcgg cgatgagatg gtgatgcttt ccggcgcggc gctgccggac
     6961 cgggtgaagc tcgtcgccgc gctcaaggcc gcgcggctgt ccgctcagcc gcagtctcgc
     7021 ctaccccagg gaatcaccta cgaggcggaa cagaactggc gcgtgactgg cggggaggtg
     7081 gaagtgagcc ggggcgggca cgcgaacgtc tccggcgagg ccaacctctg gtcgtggaac
     7141 acgcccggga gcacgatctc gtgggagatc gaggtgaagg aggcaggaac ctacgagatc
     7201 tggttcgtgg gcgcgacgga aggcggcctg ctcgcggagt tcgccgtgga tggcggcgag
     7261 gcgctggggc tgcgcttcga gccgacgggc ggctggggcc gcaccgccgc cggagaatgg
     7321 cgcgcgttcc aggtgcgtgc cgcgtccggc actccggccc ggttcaagct ggacgctggc
     7381 cggcaccgcc tggcgctcac caaccggacc ggcgtggggc tgaacctcga ccggattctt
     7441 ctgatgccgg tccgatagcc ccaagtattc accagaatac ttcaaaaaga agtctcttgc
     7501 ctggtggcac gtcgttgccg cctcaagcac ccggaagcag agtgttttcg gagctgtgcc
     7561 cacgaacctg cgccgcgcct ggcagagaga cttcttttca ttcttctgat tgaatcatgc
     7621 gggatagttc ccggcaggcc gcgccgggga gggtttgcgc cctccccggc caatctggtt
     7681 gcgtgtccgg gggtgccagg agtgctctgg caaggtggag aatgatagag acaacgtggg
     7741 cacgccgccg cgcgggtcca caggcgttct ttccagcagg aggtttgaga tgacctgtcc
     7801 gcgatactct ttcccatggc gcctggcgac cggattggtc gcggcgttgc ttctgctcgt
     7861 cgcagcaccg tcggcctggt ccgcggagga ggagcagaat ctcgtcacca atccgggttt
     7921 tgaggagggc aacaccggct ggaacttgcc ccagacctac accgtggtcg acgacgtggc
     7981 ccattccggc aagcgcagcc tgcgcgttgt gaacaccgat ccggatcgtt acctgctggc
     8041 aagccagccc ttgaagctca agccggggat gatgtaccgg ttttccgcgt gggtgcgcgc
     8101 ccagggcgtt caggggaatg acaccggcgc gacgatctgc gtggaatggt atggcgagaa
     8161 gggcttcatc gggggcacct accccggcgg gatcaagggc gaccgtgact ggtaccgcat
     8221 cgaggcggtc actcccccga tccccgaggc ggccacgcgc tgccaggtca cactctatct
     8281 ccggaagaag atgactggca cggcatggtt cgatgacgtc agcgtggtag ctgtctaccc
     8341 gcctgctctg gacgcggcgc ttgtcttccc gaactaccgg ggtatcctgt ggcccgattc
     8401 cgacaagcag caggtagtgt tgcgcgggca ggtgggcgcc tatctgaagg gcggcctccg
     8461 ccccgagcag atgacgctcg ccggctcgct gctggatggg gagaaggtga tcgacgagcg
     8521 gcggattcgg caggtcggtg ccgggatgca tgaggtaact ctgaacgcgc gcgggcttcg
     8581 ccccggcaac tacatcgcgc gcatggagct tctcgcgccc gatggaaagc cactagagcg
     8641 caaagagttc gcggtccgga agctcgcgcc tggcacgccc cggcccaagg tctatatcga
     8701 cgagcacaac cggacaattg tagacggcaa gccgttcttc ccgctcggct ggtacttcgg
     8761 gccgggcccc ggcaacgaag gcttccagga gcacatcgat cgcgtcgccg agagcccgtt
     8821 caacacgatc atgtgctacg ggatcaacta tggcggccaa gagaaggt
//
LOCUS       DAOSCV010000039         8818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5190_length_8818_cov_3.674084, whole genome shotgun sequence.
ACCESSION   DAOSCV010000039 DAOSCV010000000
VERSION     DAOSCV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5190_length_8818_cov_3.674084"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>882
                     /locus_tag="PLY56_01835"
     CDS             <1..>882
                     /locus_tag="PLY56_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20249.1"
                     /translation="QGIEKLDLSGTYALTSRLSAQTAYQRTEEAADGQTARVKTLLSN
                     ALTADITPLSRLTLRRQEEEVSAPGDSESTKITERVQLDQRIGKMGTAQVHHEVMVDG
                     SGSEEQTTQTTGMKLDARVTNSTRVVAQRLETRAEDQEPAIQTQVALSAGISGGKIKF
                     EGEYLEHERPGLAAEEIQGARVEATPLGGVKIGGGVRQLQTGEQVQLQKHAQIEVRAP
                     AGILLAGRLQEQTDGTGNMLGLVRAVDATMKPLQGIEMSGTYKEREERDQRGPFTRSL
                     RIRLAPVKFLQLTGGLI"
     assembly_gap    883..892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <893..1260
                     /locus_tag="PLY56_01840"
     CDS             <893..1260
                     /locus_tag="PLY56_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20250.1"
                     /translation="EQGVVLQERRRTMGLRSELGWLTFTGDLAQRYDGSARPLAEELR
                     LGMTLNLSAYDRLFTTYKVAEETGATALTTVHYGIGYSRRVANALDLSLESELLERLD
                     ENRTEEDEVRARASLNMRF"
     gene            complement(1308..2966)
                     /locus_tag="PLY56_01845"
     CDS             complement(1308..2966)
                     /locus_tag="PLY56_01845"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOJ20251.1"
                     /translation="MNLPDIWGPGQLLAFSGIDGHTDWAKPFVLHTGARPGGLLVRLP
                     ATIAVGFEGMPPLRFQAILGDAITAESPGGAYRAVFLDHHTLAGELPEGTRMTVDGRS
                     AGEDALSVDAGPMKLVAATRGRRWALLLSDASATDVAERLTQALGADLTPVFEQREAF
                     VRRVRIPEGLSPERERLLRKAVSVMKVNTEAPCGVIRRRWTTPDRWPHRHMWLWDSAF
                     HSMGIAYVDMDLAKDAVLAMLEQVHEDGLLPHMVPAGGTPSTITQPPVLAWATLRILR
                     LSGDVEWAAECLPYLHRYLEWMRLNRDKNGNGIPEWHIAGDPLCRSGESGLDNSPRFD
                     RAVLLDAVDFASFLSHDLECLGEIAARVGNQALQAECEAHARRIAEAVNALLWSDDEG
                     FYLDRDFEGRLSEVKAVTGFLPLLAGIPNAGQAEALCQHLSNPRTFGAPLPVPSVSLD
                     SGSYCKDMWRGPTWMNLNYAILCGLLRAGFHEEAAQLRERSLAAIQRIYETDGCLYEY
                     YDSLDVTHPSRLDRKQRLISGKGIAPISDYHWTAALTAAMIFEG"
     gene            3309..5201
                     /locus_tag="PLY56_01850"
     CDS             3309..5201
                     /locus_tag="PLY56_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20252.1"
                     /translation="MILVLIGLLVRHTEIITGRYVSSGVPPVLAFAGLLLFLGARPLL
                     RRIHPRLCPSRSQALLVYMMLTIGVWLSGAYGVRAFLPHLVALQYWSQSDANLAPYPG
                     ELPEWYAPRDAEAARAYFEGSRDGQVPWGVWLPVLLRWIPFMLALFGGAFCLMLLMRR
                     QWLQSERLTFPLLYLPLSLASEGGSRFTGDGKPLLRSPILWAGISIAILFNALNIAHA
                     LIPTVPAPKFSYSLAPHFPNRPWTPLRSITLFYMLETIGFGYFVPLEISFSAWFLYLA
                     EKAFAVAGTAAGYDKPGYPFIQDQCAGAYLACGLMLIWGARRHLGAMWRRAWMHAGEE
                     RVAWVGLGVCSVVVVGWAVAAGLALEVAIPYFLVLGCFVLVYARIRAETGVPLEFIYP
                     YGLPKEMVLNALSVPGITGVGGVRSMVIFSSLAWLARHHMTETLAAYQIDSLKLNDQE
                     RIGRRKLAVALGVAFAIGLGAAVWAHLDGYYQIGSNAASGGMGEYRATVARQEYQQMA
                     SRITAPPLRDWTRITANASGFGIATALAAVRARVPGFPLHPLGFLLATAYGDHTTCFF
                     PLFVAWLAKLIILKAGGLKLYRRGIPFFLGLIIGHFTLAGILWPLLSMLISPEASRSY
                     HIFFGG"
     gene            complement(5203..7050)
                     /locus_tag="PLY56_01855"
     CDS             complement(5203..7050)
                     /locus_tag="PLY56_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ20253.1"
                     /translation="MDRFAQENPNLSPGRPTALPHRRSWGLLWAILVAALLAWRPAQG
                     APAIYAPYLTDTPPRILEVLEKKTEEGVEITRLKFLNRELPDGEKVIIYGILARPATP
                     GRYPGVLVCHGGGGYADGVAPAVKAWAKRGYVSFCQDQPGICNMRQASSSGPWSAKKW
                     GLFTVEPDPTCNILFDGVVAALNGLALLRSQPDVDRSRIGVTGGSWGGYMTTMVSGLA
                     GDRVRAAFSIYGCGFYDEGSHWSHTIGGLPEETRKRWVEHLDAGRQAKGIRANYFVAA
                     AANDWFFWPHIVMRTLNEIPAPKNLVFSPNDSHCILVPGGTSGPPAFDHQGHRTYMEI
                     LWMDYHLKGAGEPFPVCQPIGAPRRKGDAIEVRFQVTGPKPVREASVWVAPAEMPWRL
                     KWWRKVEVQPSGDGLYTALLPVEETEYGLNWFGLATDERYISTSTPMQEVQPAALGFK
                     PEERRDVRFAEDFEGPEAAKRWRRQYWPEEKYKNGRALVCAEAARTGKRGLKITQQFA
                     LRGDGIRGVALRASGTRGLAFWVRSPGGTGFDVQLMGEMPDGKRIYWQSSQPDPGKEW
                     KRIEIPWSEFKLVDEETAPFEPLSPGLGQIRFVTPENAEIHIDDLEAIR"
     gene            7441..8037
                     /gene="infC"
                     /locus_tag="PLY56_01860"
     CDS             7441..8037
                     /gene="infC"
                     /locus_tag="PLY56_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOJ20254.1"
                     /translation="MTAIREGLGIDRDRELRVNHRIRAREVRLIDEEGEQRGIVALRY
                     ALELARDKGLDLIEVAPHAVPPVCKIMDYGKFKYEQSKRESEARKKARASELKTVRME
                     PQIDDHDFQFKLRSIQKFLKEGDKVKISVMFRGRSITHPEFGKRLLEQVVQQCAEIAA
                     VERPPAFEGRMMTLILSPRQQPQGGAAQPQPAQAQPRA"
     gene            8143..8340
                     /gene="rpmI"
                     /locus_tag="PLY56_01865"
     CDS             8143..8340
                     /gene="rpmI"
                     /locus_tag="PLY56_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOJ20255.1"
                     /translation="MPKLKTRKGVAKRFQVTGSGKIMRRKAFKGHLMLHKSPGQRRRL
                     GQEAEVTGGFAKEIRRNLPYA"
     gene            8431..8814
                     /gene="rplT"
                     /locus_tag="PLY56_01870"
     CDS             8431..8814
                     /gene="rplT"
                     /locus_tag="PLY56_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ20256.1"
                     /translation="MARVKRGVMTRKRHNQVLKLAKGYRGAKHRLFRQAHEAVMHAGQ
                     YAYRDRRQRKRDLRRLWITRISAACKLNGITYNRFIEGLTKAGVEVDRKILADLAVTD
                     ASAFTEMVGLARQHATLPGAQAAAA"
BASE COUNT         1491 a         2810 c         2965 g         1542 t
ORIGIN      
        1 agcagggtat cgagaagctc gatctctccg gcacctacgc gctcacgagc cgcctctccg
       61 cgcaaacggc gtaccagcgc accgaagagg ccgccgatgg ccagacggcg cgcgtgaaaa
      121 cccttctctc caatgcgttg accgccgata tcactcctct ctctcgtctg acgctgcgcc
      181 gccaggagga ggaagtgagc gcgcccggcg attcggagag cacaaagatc accgagcgcg
      241 tgcagctaga ccagcggatc gggaagatgg gcaccgccca ggtgcaccac gaggtgatgg
      301 tcgatggcag cggcagcgag gagcagacga cccagacgac gggcatgaag ctggacgcgc
      361 gcgtgacgaa cagcacccgc gtggtcgcgc agcggctgga gacgcgtgcc gaggatcagg
      421 agccggccat acagacgcag gtggccctga gcgccgggat ctccgggggc aagatcaagt
      481 tcgagggcga gtatttggag cacgagcgcc ctggcctggc cgccgaggag atccagggcg
      541 cccgagtgga agcaactccg ctcgggggcg tgaagatcgg cggcggtgtg cgccaattgc
      601 agaccggtga gcaggtccag ctccagaagc acgcgcagat tgaggtgcgc gcgccggcag
      661 gcatcctgtt ggcaggccgc cttcaggagc aaacggacgg cacagggaac atgcttggcc
      721 tggtgcgggc ggtggatgcc accatgaagc cgctccaggg catcgagatg tctggcactt
      781 acaaggagcg cgaggagcgc gaccagcgcg gtccattcac gcgcagtctc cgcatccggc
      841 tggcccctgt gaagttcctc cagttgaccg gcggtctcat ccnnnnnnnn nnaagagcag
      901 ggagtggtgc tccaggagcg gcggcgcacg atgggcctgc gctccgagct gggctggctc
      961 accttcaccg gcgatctggc acagcgctac gatggatcgg cccgcccatt ggccgaggag
     1021 ctgcggctgg gcatgacact gaacctatcg gcgtatgacc gccttttcac tacctacaag
     1081 gtggcggagg agacgggtgc cacggcgctc acgacggtcc actacgggat tggctactca
     1141 cgccgggttg ccaacgcgct ggatctctcg ctggagagcg agttgctgga gcgcttggac
     1201 gagaaccgca cggaggaaga cgaagtgcgc gcccgcgcat ccctcaacat gcgcttctaa
     1261 aggcccctgg gtcacgcccg cggcggggct ccattcgtgc ccgcggtcta tccctcgaag
     1321 atcatcgccg cggtcagcgc cgccgtccag tggtagtcgc tgatcggggc gatccccttc
     1381 ccgcttatca ggcgctgctt gcggtcgagt cggctggggt gcgtcacgtc gagcgagtcg
     1441 tagtactcat agaggcagcc atccgtctcg tagatccgct gaatcgccgc gagtgaccgc
     1501 tcgcgcaact gagcggcctc ctcgtggaag ccggcgcgga gcagcccgca gaggatcgcg
     1561 tagttcaggt tcatccaggt aggcccgcgc cacatatcct tgcagtagct gccggaatcg
     1621 agagagacgg agggcaccgg gagtggcgcc ccgaaggtgc gcgggttgct caggtgctgg
     1681 cacagcgcct cggcctgccc cgcgttcggg atacccgcca ggagcggcag gaagccggtg
     1741 accgccttca cctcgctcag gcggccctcg aagtcgcggt cgaggtagaa gccctcgtca
     1801 tccgaccaga ggagcgcatt gaccgcctcg gcgatgcggc gggcgtgtgc ttcgcactcc
     1861 gcctgcaacg cctggttccc cactcgtgcg gcgatctcgc ccaggcattc caagtcgtgg
     1921 ctcaggaagc tggcaaaatc cacggcatcg aggagcacgg cgcggtcaaa ccggggcgag
     1981 ttatccaggc ccgattcgcc ggatcggcag agcgggtcgc ccgcgatgtg ccattcgggg
     2041 atcccattcc cgttcttgtc gcggttcagg cgcatccact ccagatagcg atgcaggtag
     2101 ggcaggcact cggcggccca ctccacgtct ccggagaggc ggaggatgcg cagagtggcc
     2161 caggcgagaa cgggcggctg ggtaatggta gacggagtac ctcccgccgg caccatatgg
     2221 gggagcaagc catcttcgtg cacctgctcc agcatcgcca gaacggcgtc tttcgccaga
     2281 tccatgtcca cataggcgat gcccatcgag tggaaggcgg aatcccacag ccacatgtgc
     2341 cggtgcggcc agcggtcggg cgttgtccac cgtcggcgga tcacgccgca gggggcttcg
     2401 gtgttcacct tcatcaccga gaccgccttg cgcaagagcc gctcgcgctc gggcgagagc
     2461 ccctcgggga tccgaacccg gcgcacgaac gcctctcgct gctcaaacac tggcgtcaga
     2521 tccgcgccga gtgcttgcgt caggcgctcc gcgacatccg tggcgctggc atcggatagg
     2581 agcagcgccc agcgccggcc tcgcgtggcg gcgacgagct tcatcgggcc ggcatccacg
     2641 ctcagcgcat cctcgccggc cgaacgtccg tccacggtca tccgcgtgcc ctcgggcagc
     2701 tctccggcga gggtgtgatg gtcgaggaag acggcgcggt aggctccacc gggcgactca
     2761 gcggtgatcg cgtcgcccag gatcgcctgg aagcggagcg ggggcattcc ctcgaagccg
     2821 actgcgatgg tcgcgggcag gcggaccagc agacctccag ggcgcgcccc ggtatgcagc
     2881 acaaacggct ttgcccagtc ggtatgtccg tctattcccg agaaggcgag gagttggccc
     2941 gggccccaga tatccggcag attcatcgct tccccctgtt tcgaccggcg agaggccgcc
     3001 ggctcgccgg gggctcctcg cgcgcgcgat ttgcagagta gatacgactg tgtcggcctg
     3061 gcatcctgcc aggggatcga gtgcagcaac acccgggtat ttgcggcact agcgcgagcc
     3121 ttctcatccc gcaggatccc gggcgggaga tgacgaaccg gtcaacactc cacaacaatt
     3181 ggaaagtagc cacggttatg ccagatcacg agaagcactc ccccaacagt cttggggctt
     3241 ccgctggaga atcgcagtca cagtcactga catggaaggg cgccctcgta tctatcatcg
     3301 ctggcgccgt gatcctcgtc ttgatcggcc tgttggtgcg gcacacggag attatcaccg
     3361 ggcgctacgt gagctccggg gtacctccgg tgctggcctt cgcggggctg cttctcttcc
     3421 tgggagcacg cccgctcctg cggcggatcc acccgcgcct gtgccccagt cggtcgcagg
     3481 cgttgctcgt ctacatgatg ctgaccatcg gggtgtggct ttcgggcgca tatggcgtac
     3541 gcgcattcct gccccacctg gtggcgctgc agtactggag ccagtcggat gcgaatctgg
     3601 cgccctatcc tggggagttg ccggagtggt acgcgccgcg cgatgccgag gcggcacgag
     3661 cctacttcga gggctcgcgc gacggccagg tgccgtgggg cgtgtggctc cccgtgctcc
     3721 tacgctggat tcccttcatg ctcgcgctct tcgggggcgc gttctgtctc atgttgctga
     3781 tgcgccggca gtggttgcag tcggagcgtc tcacctttcc gcttctctac ctgccgctct
     3841 cgctggcctc ggaggggggc tcgcgcttta cgggagatgg gaagccgctg ctgcgtagcc
     3901 cgattctgtg ggcagggatc agcatcgcga tcctcttcaa cgcgctcaac atcgcgcacg
     3961 cgctcattcc gacggtgccg gcgccgaagt tctcctactc gctcgcgccc cacttcccta
     4021 accggccctg gactccgctg cggagtatca ccctcttcta catgctggag acgattggct
     4081 tcggctattt cgtgccgcta gagatcagct tctcggcgtg gtttctctac ctggcagaga
     4141 aggcgtttgc cgtggcgggc acggctgctg gatatgacaa gccaggctat cccttcatcc
     4201 aggatcagtg cgccggcgcc tacctggcct gcgggctgat gctcatctgg ggtgcccggc
     4261 ggcacctggg ggcgatgtgg cggcgagcct ggatgcacgc tggagaggag cgcgttgcct
     4321 gggtcgggtt gggcgtctgc agcgtggtgg tcgtcgggtg ggcggtggcc gccggcctgg
     4381 ccctggaggt cgcgatcccc tatttcctgg tgctgggctg tttcgtcctg gtctacgcgc
     4441 ggattcgcgc cgagacaggc gtgccgctgg agttcatcta cccctatggg ctgcccaagg
     4501 agatggtgtt gaacgcgctc tcggtgcccg gcatcactgg cgtggggggc gtgcggagca
     4561 tggtgatctt ctctagcctg gcgtggctgg cgcgccacca catgaccgag acgctggcgg
     4621 cctaccagat tgacagcctc aagctgaatg accaggagcg gatcggccgg cgaaagctcg
     4681 cggtagctct gggggttgcc tttgccattg gccttggcgc ggcggtctgg gcgcacctgg
     4741 atggctacta ccagatcggg agcaacgctg cctccggtgg gatgggggag taccgggcga
     4801 cagttgcccg ccaagagtac cagcagatgg ccagccggat cacggcgccg ccgctacgcg
     4861 attggacgcg cattaccgct aacgccagtg gctttgggat cgccacggcg ctggccgccg
     4921 tgcgggcgcg ggtgccaggc ttcccgctcc acccgctcgg ctttctgttg gccacggcct
     4981 acggcgacca cacaacctgc ttcttcccac tcttcgtggc ctggctggcg aagctgatca
     5041 tcctgaaggc aggcggtctc aagctctacc ggcgcggcat cccgttcttc ctcgggctga
     5101 tcatcggcca cttcaccctc gccgggatct tgtggccgct tctcagcatg ctgatctcac
     5161 cggaggcgag ccgctcctac catatcttct tcggaggctg agctaccgga tcgcctccag
     5221 gtcgtcgatg tggatctcgg cgttctcggg agtgacgaag cggatctgac ccaggccggg
     5281 cgagagcggc tcgaacgggg cggtttcttc gtccacgagc ttgaactcgc tccaggggat
     5341 ctcaatccgc ttccactcct tgccggggtc gggctggctc gattgccagt agatccgctt
     5401 gccgtccggc atctcgccca tgagctggac atcgaagccg gtgccgccgg gcgagcgcac
     5461 ccagaaggcg agcccgcgcg tgccactcgc gcgcagcgcg acgccgcgaa tgccatcgcc
     5521 gcgcagagca aactgctggg tgatcttcag gcctcgcttg cccgtgcgcg cggcctccgc
     5581 gcagacgagc gcccgaccat tcttatactt ctcctccggc cagtactgcc ggcgccaccg
     5641 ctttgccgct tccgggccct cgaagtcctc cgcgaagcgg acgtcgcgcc gctcttccgg
     5701 cttgaagcca agtgctgccg gctggacctc ctgcatcggc gtagatgtgc tgatgtagcg
     5761 ctcgtcggtt gccagtccga accaattcag gccatattct gtctcttcga cggggagtag
     5821 ggccgtgtag aggccgtcac cagagggctg aacctccacc ttgcgccacc acttcagacg
     5881 ccagggcatc tcggctgggg ctacccaaac gctcgcctct cgcaccggct tggggccggt
     5941 cacctggaag cgcacctcga tggcgtctcc cttgcggcgg ggcgccccga tgggctggca
     6001 gacggggaac ggctcgccgg cccccttcag gtggtaatcc atccagagga tctccatgta
     6061 ggtgcggtga ccctggtggt cgaacgcggg agggccggag gtgccgcccg gcaccaggat
     6121 gcagtggctg tcgttgggcg agaaaaccag gttcttaggc gccgggatct cgttgagcgt
     6181 gcgcatgacg atgtgtggcc agaagaacca gtcgttggct gcggcggcga caaagtagtt
     6241 tgcgcgaatc cccttcgcct gccggccggc atcgaggtgc tccacccacc gcttccgcgt
     6301 ctcctctggc aaccctccga tcgtgtggct ccagtgcgag ccctcatcgt agaaaccgca
     6361 accataaatg gagaacgcgg cgcgcacccg gtcgcccgcc aggccgctga ccatggtggt
     6421 catgtagccg ccccagctcc caccggtgac gccgatgcgc gaccgatcca cgtccggctg
     6481 cgagcgcagg agcgccaggc cgttgagcgc cgcgaccacg ccatcaaaca ggatgttgca
     6541 cgtgggatcc ggctccacgg tgaagaggcc ccactttttc gccgaccacg gccccgagga
     6601 tgaggcctgg cgcatgttgc agatgcccgg ctggtcctgg cagaaggaga cgtagcccct
     6661 cttggcccag gccttcaccg cgggcgcgac gccgtcggcg tagccgccgc cgccatggca
     6721 gaccagaacc cccggatacc ggccgggcgt cgcggggcgt gccaggatgc cgtagatgat
     6781 caccttctcc ccatcgggca actcccggtt gaggaacttc aggcgggtga tctccacgcc
     6841 ttcctccgtc ttcttctcca gcacttccag gatgcgtggc ggggtatccg tcaggtaggg
     6901 cgcgtagatc gcgggcgcgc cctgtgccgg gcgccatgcg agcagtgccg caaccagaat
     6961 cgcccacaac aggccccagc tccggcgatg gggcagggcg gtcgggcggc caggggagag
     7021 gttcggattc tcctgcgcaa atcggtccat cttcatttcc gtcttccatc tctgtgacgc
     7081 cgagacgcca ccgggcccga gcgcggggtc tggatgcgcc cgcgcgattg gcccgaagtt
     7141 ccgcatcggc tcgtgctcca gccggtggcc tacttcggcg ttccagcgcc gattacctgt
     7201 agaagttgtg ggggatctgg cggaggggcg ggaacccttt cgcccccgag ggcatcctga
     7261 acaatgtctg tctatggcgg gtgtgcatgg aatgccttgg taaaaaacca cgaattcagg
     7321 cgcggcacac gttgacaagc ccccgtccct gtggtatact ccgcgtatct atagcgcagg
     7381 cgagagcggt cggccgtctg agcgatccgt acgcccgcag gagaagagaa ctcgagagcg
     7441 atcaccgcga ttagggaggg actgggcatc gacagagaca gagaactacg ggtaaaccat
     7501 cggatccgcg cccgtgaggt gcgcctgatc gacgaagagg gggagcagag gggcatcgtc
     7561 gcccttcgct acgctctaga gctggcgcgc gataagggcc tggatctgat tgaggtggct
     7621 ccgcatgccg taccgcctgt ctgcaagatc atggactatg gcaagttcaa gtatgagcag
     7681 agcaagcggg agagcgaggc gcggaagaag gcacgggcgt ccgagctgaa gacggtgcgc
     7741 atggagccgc agatcgatga tcacgacttt cagttcaaac tgcggagcat ccagaagttc
     7801 ttgaaggaag gcgataaagt caagatctcg gtgatgttcc gtgggaggtc gatcacgcac
     7861 cccgaattcg gcaagcgctt gcttgagcag gtcgtgcaac agtgcgcgga gatcgccgcc
     7921 gtagagaggc cgcctgcctt cgaggggcga atgatgacgt tgatcctcag tcccaggcag
     7981 cagccgcaag gaggggcggc gcagccccag ccagcgcaag cacagccgcg agcgtgagtt
     8041 gctgcatgta gatttagggt aatcggactg ttccgcgggg ggtctgcagg tgctgtgaga
     8101 ccggccgacg ggacagttcg tgtctatgag aaggggatgg ccatgcccaa gttgaagacg
     8161 cgcaaagggg tggcgaagcg cttccaggtg accggttcgg gcaagatcat gcgccgcaag
     8221 gcgttcaagg gacacctgat gcttcacaag tcgcccggac agcgccgccg cctcggccag
     8281 gaagccgagg taactggcgg atttgctaaa gagattcggc gcaatctgcc gtacgcttag
     8341 aacctgtcca ttgtggttgt cctcgggcgg gcttcgggtc gatgccgagg ggtattccag
     8401 acaggctctt gaccatttgg agaggtaacg atggcacgag tgaagcgcgg ggttatgacc
     8461 cgcaaacgcc ataatcaggt cttgaagctg gcaaagggtt accggggagc caagcatcgg
     8521 ctgttccgcc aggcccatga ggccgtgatg catgctgggc agtatgccta ccgcgaccgg
     8581 cggcagcgca agcgggatct ccgacggctt tggatcactc gcatcagcgc cgcgtgcaag
     8641 ttgaacggca tcacgtacaa ccgctttatc gagggcttga cgaaggccgg ggttgaagtg
     8701 gaccgcaaga tccttgccga cctggcggtg accgatgcca gcgccttcac cgagatggtg
     8761 gggctggcgc gccagcatgc cacgcttccg ggcgctcagg ccgcagctgc ctagtttg
//
LOCUS       DAOSCV010000040         8777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5236_length_8777_cov_3.213827, whole genome shotgun sequence.
ACCESSION   DAOSCV010000040 DAOSCV010000000
VERSION     DAOSCV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_8777_cov_3.213827"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PLY56_01875"
     CDS             complement(<1..1417)
                     /locus_tag="PLY56_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HOJ20257.1"
                     /translation="MPVSEPVTHVFGIRHHGPGSARSVLKALEALQPDIILVEGPPDA
                     EEVLPLLTHAEMEPPVALLLYVPEEPSRSVSYPFALFSPEWQALRYGLTRGIPTRFID
                     LPQAHQLAMREDAGAAGSEAVEDESDPVAPPEPAEVVRQDPLGWLAAAAGYSDGERWW
                     EHMVEQRLDGDDLFAGICEAMAALRAEMPREEDPLEERREAYMRRCIRQARKEGYERL
                     AVVCGAWHAPALAEMPPASHDAKLLTGLPKVKVSATWIPWTYGRLARSSGYGAGIESP
                     GWYHHLWSDSDRVAIRWMTRVASLMRAHDLDASAAHVIEAVRLAESLAALRDRPLPGL
                     DELNEAVRAVFCFDSDLPMRLIEEQLIVGERLGKVPEVTPQVPLQQDLARLQKRLSLP
                     PEATQKQLDLDLREPLGLARSHLLHRLALLGIPWGQPERVTGKQSTFHEIWTLQWHPE
                     FAVALIEAGVWGNTVAAAAAAK"
     gene            complement(1512..2591)
                     /locus_tag="PLY56_01880"
     CDS             complement(1512..2591)
                     /locus_tag="PLY56_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ20258.1"
                     /translation="MTNILRQHAEQQYAEELEALAEADSRPRPPNWRLSPWAVVTYLL
                     GGKLENGFAVTPKYIGNRRLIEIAVATLATDRALLLLGVPGTAKTWVSEHIAAAIAGD
                     STLLIQGTAGTSEEQIRYGWDYARLLSEGPSRRALVPSPLMRAMQQGKIARVEELTRI
                     PADVQDTLITILSEKTLPIPELGEEEQAVKGFNVIATANNRDRGVNELSSALKRRFNT
                     VVLPVPESADEEVEIVQRRVESLGRALELPAPPPGVQEIRRIVTIFRELRNGVTADGK
                     TKVKSPSGTLSTAEAISVVSNGMALAAHFGDGTLRAADVAAGLIGAVVKDPVQDRVVW
                     QEYLETVVKERDGWKDLYRACRDVS"
     gene            complement(2591..4147)
                     /locus_tag="PLY56_01885"
     CDS             complement(2591..4147)
                     /locus_tag="PLY56_01885"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HOJ20259.1"
                     /translation="MGEQGASSHTAWNELVAAALIGGEREAVRLPDEGPLGEVLSRLE
                     CPSREQQLLGAAAILSLYRRAGARPAVETAPPPEPSPAESLSRCPSSVGRYLAEILSG
                     TRAELLSEWLDAAAAAGMRAPEELLPALLDLGAAREGIREKLLPVLGERGRWLARHNP
                     DWAYALGPEEAIDWETAPRVARLALLKRLRATDPARARELLQSTWEQDPPNERAAFIE
                     HLNAGLSMEDEPFLEAALDDKRKEVRTQAAELLAKLPQSRLSARMLERVRPLLSFTPG
                     QPSRVLAFMPGKKPSLQVTLPEACDRAMARDGIEAKPPPGMGKKAFWLMQMIAVIPPA
                     TWCEMWGTPPQVLIEAARETEHHRELLEGWARAAARHRDREWAEALLSTSIPSLPEGV
                     AAELVDSLPPERREAALLPHVQASGNRIPLVPLLAHCQHAWSEMFTRAVIALVREHVR
                     GPGYPSDWRIHDALKEFALYAAPALADEATRTIGGPPGSMQLRSVEQFLDLLHFRRQM
                     LAAFGDKETR"
     gene            complement(4217..5536)
                     /locus_tag="PLY56_01890"
     CDS             complement(4217..5536)
                     /locus_tag="PLY56_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HOJ20260.1"
                     /translation="MSLTWTTEQVLALAPDAASAKAGRELATPRKWVSLGRDRQSAWG
                     LCQGSGKSPYQTQIDLGEPAFRCSCPSRKFPCKHSLGLFLLLAAQPEAFPEQTPPAWV
                     TEWLEARAKRAEQRAAKSTRKEEPADPAAQERRAARRESRVAAGLQELDLWLCDLVRS
                     GLASAQGKPPAFWEGIAARLVDAQAPGLARLVRDLSSLPASGTGWQERLLERLSLIYL
                     LIEGYRRIDTLPAETQADLRSLIGWTQSQETLLAEPGLSDHWAILGQRIEQEEHLRVQ
                     RTWLQGTESGRPAMILHFAHGTAPLDTSLVPGTCFNGEVVFFPGAYPLRALIKSREGA
                     AEPLQAMGPGQTIASAAEARAAALARNPWISRFPVLLETVVPTYREGTWVLRDTEGSL
                     LPLHPRFDRGWQLLALSGGYPLALFGEWEEDHFLPLSAWADSVFLGL"
     gene            5700..>8777
                     /locus_tag="PLY56_01895"
     CDS             5700..>8777
                     /locus_tag="PLY56_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ20261.1"
                     /translation="MALVVSLIALLMWIALPAGHAQAIPENLVSRARVSASSQASPAD
                     GKYGVLRLADGNRATHWASANKALPQWIRIEWDQAQEFDTISLQIYHTQARNLYAGWK
                     RFEVELSDGTKRAYTVQPDQDVVNARLEKPCRAKWVQITLLEVYEERTYVGVDEVGIY
                     LDPERRIREPKPIARALPREAIRVLGGRPHPSVYVTAADVARARRNAEQTEWGRQTKQ
                     EILAAADKWLERTEEEWLRFLPPPGACYAYGFTGCPICRSSWGTWGGARCSWDRPGTV
                     QCTQGHILPDAKHPDDGSGYKGPDGRVHYFVGSWNAWVTEQWLNAINRLGHAYALTGD
                     ERYAERAAFFLDALASIYAESTSGSWDYPSSPPSGRFARPWYQVARTLVPFVEGYDLI
                     YTSKALDKPSLRPRLEKGFPKGPTLQQRVVGTPDARGKSWDGMTRRDNIDVNLMQDGA
                     YYCYSHSFSGMLHNGHADYMRGALAVGVLLGIPTYVYNAVESPYSIYAMLANNCDRDG
                     RYYETALGYAIHCRDLYLTFTEPLRHWSDERYPKGVNLFANDRFRAFYELPDLTMDIA
                     GHALNYGDCSPDHAFVFSSDDKFSQTDYAFAERLYAGCTGAERERFARLVRYLAGGDV
                     ERARAAASNRIWLLYHADPVPGNEKPSLPEDLHRKVFGSWFLGQKGLAILRDGSGAEA
                     QGALVRFGPSLNHGHLDDLGLIYYAKGWQCTYEIGYGLGSTHTQVGWARQTASHCLVT
                     VDEARQSGGSGGSLHLFARLPGIKIVEADSPLSYAANGVTQYRRTVALIGEGKDQYLV
                     DLFRVRGGRQHDYILGSQGQEFEVSGVELGPEEKGSLAGLEHAWGEKLGNDGDIIGMP
                     NKPYWNPPPGNGYGFFYGMRRGRAAGPWWVDWALGGPNQARFRAHVLPEASAEAIVAR
                     APGLYPRNRNASYLLLRRTGENLQSCFATVLEPYALPMPSGMADYTRLLRSVTANQGA
                     VVPMHSLAALLLRGTAPGDYVECALSVPTAGEYEVQARLYRYSSYGAVRLSVDG"
BASE COUNT         1355 a         2969 c         2991 g         1462 t
ORIGIN      
        1 ccttcgcggc agcggcagcg gccacggtgt tgccccagac gcccgcctcg atgagcgcca
       61 cggcaaactc cggatgccac tggagggtcc agatctcgtg gaacgtgctc tgctttccgg
      121 tgacgcgctc gggctggccc caggggatgc cgaggagggc gagccggtgg agcagatggc
      181 tgcgtgccag cccgaggggc tcgcgcaggt cgaggtcgag ctgcttctgg gtggcctcgg
      241 ggggcaggct caggcgtttt tgcagccgtg ccaggtcctg ttggaggggc acctggggcg
      301 tgacctccgg caccttgccc agcctttcgc cgacgatgag ctgctcctcg atcaggcgca
      361 tcgggaggtc gctatcgaag cagaagacgg ctcgcaccgc ctcgttcagc tcatccaggc
      421 cgggcaaagg gcggtcgcgc aaagccgcga gcgactcggc caggcgcacc gcctcgatga
      481 cgtgcgcggc agaagcatcc agatcgtgcg cgcgcatcag gctggccacg cgcgtcatcc
      541 agcgaatggc gacgcggtcg gaatcgctcc agaggtggtg ataccacccg ggcgattcga
      601 tcccggcccc gtagccgctg gaacgggcga gccggccgta ggtccagggg atccaggtcg
      661 cgctcacctt caccttgggg aggccggtga gcagcttcgc gtcgtgggag gccggtggca
      721 tctccgcgag cgccggcgcg tgccaggcgc cgcacaccac ggcgaggcgc tcgtaacctt
      781 ccttccgtgc ctggcggatg caccggcgca tgtaggcttc ccgccgctcc tccagcgggt
      841 cctcctctcg cggcatctct gcgcgcagcg ccgccatcgc ctcgcagatg ccggcaaaga
      901 ggtcatcgcc atccaggcgc tgctccacca tgtgttccca ccagcgctcg ccgtcactat
      961 agcctgccgc cgctgccagc cagcccaacg gatcctggcg caccacctcc gcgggctcgg
     1021 gcggggctac cggatcgctc tcatcctcga ccgcttcgga tccggcagcc cccgcatcct
     1081 cccgcatcgc gagctggtgc gcctgcggca ggtcgataaa gcgcgtggga atgccgcgtg
     1141 tgaggccata ccggagcgcc tgccactcgg gcgagaagag ggcaaaggga tacgatacgg
     1201 agcggctggg ctcctccggc acgtagagga gaagtgccac cggcggctcc atctcggcgt
     1261 gggtcaggag cggcagcacc tcctcggcat ccggtggccc ctcgaccagg atgatatccg
     1321 gctgcagtgc ctccagcgcc ttcaagacgc tccgcgccga cccggggccg tggtggcgta
     1381 tgccgaacac gtgggtgact ggttcggaca ctggcatctc ctccatcaag cgtcattcgt
     1441 aacgcgctgc tgaagtgggc ccggccgtgc ggcgccccgc tgggagcgcc gcgcgcgggt
     1501 ggcgcccggc atcacgagac atcccgacac gcccgataga gatccttcca gccatcgcgc
     1561 tccttgacga ccgtctccag atactcctgc cagacgacgc gatcctggac cgggtccttg
     1621 acgacggcgc cgatcaggcc ggcggccacg tcggcagcac ggagggtgcc gtcgccgaag
     1681 tgcgccgcga gcgccatacc gttgctgacc acggagatcg cctcggcggt gctcagcgtg
     1741 ccgctgggcg atttcacctt cgtcttgcca tcggcggtga cgccgttccg cagctcccgg
     1801 aagatggtga cgatccggcg gatctcctgc acgcccggag gcggcgcggg cagctcgagg
     1861 gcgcggccca ggctctcgac gcggcgctgg acgatctcca cctcttcatc ggcgctctcc
     1921 ggcaccggga ggaccacggt gttgaagcgg cgcttcaggg cgctggagag ctcattgacg
     1981 ccccggtcgc ggttgttcgc cgtggcgatg acgttgaacc ccttcaccgc ctgctcctcc
     2041 tcgcccagct ccgggatcgg gagtgtcttc tccgagagga tggtgatcag cgtgtcttgc
     2101 acgtcggcgg ggatgcgcgt caactcctcg acacgggcga tcttcccctg ctgcatggca
     2161 cgcatcaacg ggcttggcac aagcgcccgg cgcgagggcc cctccgagag aagccgggcg
     2221 tagtcccagc cataccggat ctgctcctcg ctcgtgcccg ccgtgccctg aatgagcagc
     2281 gtcgaatcgc ccgcgatggc cgcggcgatg tgctcggaga cccacgtctt cgcggtgcct
     2341 ggcacgccca gcaagagaag tgcccgatcc gtcgccaggg tggcgacggc gatctcgatc
     2401 aggcggcggt tgccgatgta cttcggcgtc accgcgaagc cgttctccag ctttcccccg
     2461 agcaggtagg tgaccaccgc ccagggcgat agccgccagt tcgggggacg cgggcgggag
     2521 tctgcttcgg ccagcgcttc cagctcctcg gcgtactgtt gctccgcgtg ctgtcgcagg
     2581 atgtttgtca tcatctggtc tccttatcac cgaaggccgc cagcatctga cggcggaagt
     2641 ggagcaggtc caggaactgc tctacagagc gcagctgcat cgaccctggc ggcccgccga
     2701 ttgtccgtgt cgcctcatcc gccagcgcgg gcgccgcata gagggcgaac tccttcagcg
     2761 cgtcatggat gcgccagtca gagggatagc ccggcccgcg aacgtgttct cgcacgaggg
     2821 cgatcaccgc gcgggtgaac atctcgctcc aggcgtgctg gcaatgcgcg agcagcggca
     2881 ccagcgggat ccggttccca gaggcctgga cgtggggaag gagcgcggct tcccggcgct
     2941 cgggcggcag cgaatccacc agctccgccg ccaccccctc gggcaagctg gggatcgacg
     3001 tggagagaag cgcctcggcc cattcccgat cgcggtgccg cgcggcggcg cgcgcccagc
     3061 cctccagcag ctctcgatga tgctcggtct ctctcgcggc ctcgatcaat acctgtgggg
     3121 gcgtgcccca catctcgcac caggtggccg gagggattac ggcgatcatc tgcatcagcc
     3181 agaacgcctt tttccccatg ccggggggcg gcttcgcctc gatcccgtcg cgcgccatcg
     3241 cccggtcgca cgcctcgggg agggtgactt gcagcgaagg cttcttgccc ggcatgaacg
     3301 ccaggacgcg cgagggctgg cccggcgtga agctaagcag cgggcgcacc cgctccagca
     3361 tgcgcgcgct gaggcgggat tggggcagct tggcgagcag ctcggcggcc tgagtgcgca
     3421 cctccttgcg cttgtcatcc aatgccgcct ccaggaacgg ctcatcctcc atgctcaggc
     3481 cggcattgag gtgctctatg aacgccgcgc gctcgttggg tgggtcttgc tcccaggtgg
     3541 attgaagcag ctcccgcgcc cgtgccgggt cggtggcgcg aagtcgcttg aggagagcca
     3601 ggcgggccac tcgcggtgcc gtttcccaat cgatcgcctc ctcggggccg agggcatagg
     3661 cccagtccgg gttgtgccgg gccagccatc ttccccgctc tcccaggacg ggcaggagct
     3721 tctcgcgaat gccctcacgc gcggcgccca ggtccaggag cgccggaagg agctcctcgg
     3781 gcgcgcgcat cccggcggcc gcggcggcat ccagccactc cgacaggagc tccgcgcgcg
     3841 ttcccgagag gatctccgcc aggtagcggc cgacggatga ggggcagcgc gagagtgact
     3901 ccgcaggaga gggctccggc gggggggctg tctctacggc ggggcgcgcg ccggcgcgcc
     3961 ggtagagcga gaggatcgcc gccgcaccca gaagctgctg ctcgcgtgac gggcattcca
     4021 gccggctcag cacctcaccc agcgggccct cgtccggcag ccggacggcc tcccgctcgc
     4081 cgccgatgag agctgcggcg accaactcgt tccaggcggt gtgagatgac gctccctgtt
     4141 cgcccatgct cctcctcaga taccaccttc aagcgaactt cctcgccggt tccgcccttg
     4201 ttcgggcgtg tgcacctcat agtcccagaa acacgctatc ggcccatgca ctgaggggaa
     4261 ggaagtggtc ctcctcccac tctccaaaga gggcgagcgg atacccgccg ctcagggcga
     4321 ggagctgcca gcctcggtcg aaccgcggat ggagtgggag caggctcccc tccgtgtcgc
     4381 gcagcaccca ggtcccctcc cggtacgtcg ggacgaccgt ctctaggagc accgggaagc
     4441 gcgagatcca cgggtttcgt gccagcgccg cggcgcgcgc ctccgccgcg ctggcgattg
     4501 tctggccggg gcccatcgct tgaaggggtt cggctgcgcc ctcgcggctc ttgatcaggg
     4561 cgcgcagcgg gtaggcgcct gggaagaaga cgacctcgcc gttgaagcag gtgcctggca
     4621 ccaggctggt atcgagcggg gcggtgccgt gcgcgaagtg gaggatcatc gccggccggc
     4681 cgctctccgt tccctgcagc caggtgcgct gaacgcggag atgctcctcc tgctcaatac
     4741 gctgacccag gatggcccag tggtcgctca ggcctggctc ggccagcagc gtctcctggc
     4801 tctgcgtcca tccgatcagc gagcgcagat ccgcctgtgt ctcggccggg agcgtgtcga
     4861 tccgccggta gccttcgatc aagaggtaga tgagggacag tcgctccagg aggcgctcct
     4921 gccagccggt gccactcgcg gggagtgacg acaggtcacg cacgagccgc gccagcccgg
     4981 gggcttgcgc atcgacgagg cgtgcggcga tcccctccca gaaggcaggg ggttttccct
     5041 gggcggaggc aagcccggag cgcaccaggt cacacagcca gaggtcgagt tcttgaaggc
     5101 cggcggcgac tcgcgattcc ctgcgggcgg cgcggcgctc ctgcgcggcg ggatcggcgg
     5161 gctcctcctt gcgggtggat ttcgcggcac gctgctcggc gcgcttggcg cgggcttcca
     5221 gccactccgt tacccacgcg ggcggggtct gctcggggaa agcctcgggt tgggcggcca
     5281 gaagaagaaa gagaccgagt gagtgcttgc aggggaactt gcggctcggg cagctacagc
     5341 ggaatgcagg ctctcccaga tcgatctgcg tctggtaggg ggacttgccg ctcccctggc
     5401 ataggcccca ggcagactgc ctgtcacgcc cgaggctcac ccatttgcgg ggcgtcgcca
     5461 gctctctgcc cgccttggcg gatgcggcat cgggcgccag ggcaagcacc tgttccgtcg
     5521 tccacgtcag agacaccgct gagtctccac ccatgcctcc cagcattgca ttcgcgctcg
     5581 ccgtgatcgc tccttctggg gagagagagg attctcattg cgcgctgggg aattgggcgg
     5641 caagcaggcg ctctctgcgt ctctcgttcc tgacccagtg gaaggaaagg gtcactgaaa
     5701 tggcgctcgt cgtctctctc attgctttgt tgatgtggat tgcacttccg gctggccacg
     5761 cgcaggcgat cccggagaac ctggtgagca gggcgcgggt ctccgcgtcg tcgcaggcgt
     5821 cgcccgcgga cggcaagtac ggtgtgttgc gcctggccga cgggaaccgc gcgacgcatt
     5881 gggcgagcgc gaacaaggcg ctcccgcaat ggatccggat cgagtgggat caggcgcagg
     5941 agttcgacac gatctctctc cagatctacc acacgcaagc ccggaatctc tacgcgggct
     6001 ggaagcgttt cgaggtggag ctctcggatg gcaccaagcg tgcctacacc gtgcagcccg
     6061 accaggatgt cgtgaacgcg cgcctggaga agccgtgccg ggcaaagtgg gtccagatca
     6121 cgcttctgga ggtgtacgag gagcgcacct atgtgggggt ggatgaagtg ggcatttacc
     6181 tcgatcctga gcgccgcatc cgagagccga agccgatcgc gcgcgcgcta ccaagggaag
     6241 cgatccgcgt cctgggcggt cggccgcatc cctcggtgta tgtgaccgcc gcggacgtgg
     6301 cacgcgcgcg ccgaaacgcg gagcagacgg agtggggccg tcagacaaag caggagatcc
     6361 tggcggctgc cgataagtgg ctggagcgca ctgaggagga atggctccgc ttcctgccgc
     6421 cgcccggcgc ctgctatgcc tacggcttca ccggctgtcc catctgccgc tcgtcctggg
     6481 gcacgtgggg cggcgcgcgc tgctcgtggg accggcccgg caccgtccag tgcactcagg
     6541 ggcatatcct gccggatgcg aagcaccccg atgacggctc tggctacaag ggccccgatg
     6601 gccgcgtcca ttacttcgtt ggctcatgga atgcgtgggt caccgagcag tggctgaacg
     6661 cgatcaaccg gctgggccac gcctatgcgc tgaccggcga cgagcggtat gccgaacgcg
     6721 ccgccttctt cctcgatgcc ctggcgagca tctacgcgga atcgaccagc gggtcgtggg
     6781 actacccgag tagccccccc agcgggcgct tcgcccggcc atggtaccag gtggcgcgca
     6841 ccctggtccc cttcgtcgag ggttatgacc tgatctatac gagcaaggcg ctcgataagc
     6901 catcgctgcg cccgaggctg gagaagggct tcccgaaagg accgacgctc cagcagcgcg
     6961 tggtgggcac cccggacgcc cgcggcaagt cgtgggatgg gatgacccgg cgcgacaaca
     7021 tcgacgtgaa cctgatgcag gatggcgcct actactgcta ttcgcactct ttcagtggga
     7081 tgctgcacaa cgggcacgcg gattatatgc gcggagcgct cgccgtgggc gtgctccttg
     7141 gcattcccac ctatgtctat aacgcggtcg agagccctta ctctatctat gccatgctcg
     7201 ccaacaactg tgaccgagat ggccgctact acgagaccgc gctcggatat gcgatccact
     7261 gtcgcgatct ctacctcacc ttcaccgagc cgctgcgcca ttggagcgac gagcgctatc
     7321 ccaagggtgt gaacctcttt gccaacgacc gcttccgcgc gttctacgag ctgcccgacc
     7381 tgacgatgga tattgccggc cacgccctca actacgggga ttgcagcccg gaccacgcct
     7441 tcgtcttttc atccgacgac aagttctctc agaccgacta tgcctttgcc gaacgcctct
     7501 atgccgggtg caccggcgcc gagcgcgagc ggtttgcccg gctcgtgcgc tatctcgccg
     7561 gcggtgatgt ggagcgtgcc cgcgccgcgg cgtcgaatcg catctggctc ctctaccatg
     7621 ccgacccggt gcccggcaac gaaaagccct cccttccgga ggacctccac cgcaaggttt
     7681 tcggctcctg gttcctcggt cagaaaggcc tcgccatcct ccgggacggc tccggggcag
     7741 aggctcaggg cgcgctcgtc cgtttcgggc cgtcgcttaa ccacgggcat ctggatgacc
     7801 tggggctgat ctactatgcc aaaggctggc aatgcaccta cgagatcggc tatggcctgg
     7861 gaagcacgca cacccaggtg ggctgggcga ggcagaccgc cagccactgc ctggtgaccg
     7921 tcgatgaagc tcgccaatcg gggggatccg gcggtagcct ccacctcttc gcgcgtcttc
     7981 cggggatcaa gattgtggag gctgactccc ccctgagcta tgccgcgaat ggggtcactc
     8041 agtatcggcg caccgtggcg ctgattggcg aggggaaaga tcagtacctg gtggatctct
     8101 tccgggtgcg tggggggcgc cagcatgatt acattctggg ctcgcagggc caggagttcg
     8161 aggtctccgg cgtagagttg ggcccggaag agaagggatc gctggccggc cttgagcacg
     8221 cctggggaga gaagctgggc aatgatggcg acatcatcgg catgccaaac aagccctact
     8281 ggaacccgcc gccggggaac ggctatggct tcttctatgg gatgcgccgg ggccgggccg
     8341 cgggtccgtg gtgggttgat tgggccctgg gagggccgaa ccaggcgcgc ttccgggcgc
     8401 acgtgcttcc tgaggcgtcc gcagaggcga ttgtcgccag ggcgcccggt ctctacccgc
     8461 gcaaccgcaa cgcgagctac ctgctcctgc ggcgcacggg agagaacctg cagagctgtt
     8521 tcgccacggt gctcgagccc tatgccctcc ccatgccctc gggcatggcc gattacaccc
     8581 ggctccttcg tagcgtgacg gccaatcagg gagcggttgt gccgatgcac tccctggccg
     8641 cgttactcct gcgcggcacc gccccggggg attacgtcga gtgcgcgctc tcggtgccta
     8701 cggcgggcga gtatgaggtg caggcccggc tctatcgcta ttcctcgtat ggcgccgtgc
     8761 gcctgagcgt ggatggg
//
LOCUS       DAOSCV010000041         8745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5261_length_8745_cov_3.859494, whole genome shotgun sequence.
ACCESSION   DAOSCV010000041 DAOSCV010000000
VERSION     DAOSCV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8745
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5261_length_8745_cov_3.859494"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..2085
                     /locus_tag="PLY56_01900"
     CDS             184..2085
                     /locus_tag="PLY56_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOJ20262.1"
                     /translation="MSAPPHSCDAFLARFLDAASALAEALPDKGGDPERLREEAADRL
                     LAGFFLRFARAASCGLQPELARLAPCAGDTPESSAAGAAESLLDALLNGYRLQLTEPR
                     PDQDALAGGALAPDILAAAHALTRAGRQDAGVFYTPVGEVRLMCREALRARMAHALGV
                     ADDDPELLGILYGQGSPDLPAERAALLFRELARLRVCDPAAGCGALLVGMAHELSRLL
                     TSLGRLVLPEDRNRLLEEGLPDPLSPTSIRRHIAASTLFGLDLDAGALNVTRQRLFLF
                     TLAAGGEQVPDVRLLEALRAHLQEGDGLEPPGQTSGSQGHDPLPTKIAGSAPTRSPWL
                     DRLPEIFAPPGGGFDIVLSNPPYVRQERLDARRKSDAPRACQALLPTEFRFDGKSDLY
                     AYFFHLGLGLLREGGALCVIASNSWLDVGFGQALRRLLLRHSRILSITESARKGTFTD
                     ARIHPAILLARRCIPAADEPEPVRFIRMHHALGEPRSLDEMTLLAGTRRFLSTPAFRV
                     VPVPQAVLAAEAVADRMGVNWGGRYLRAPDLFFEMLEACGTRLCRLDALASVRFGIKT
                     GANDFFYLESIPGARLLPPARPMTSGCFATAWGRSGSWRGASSGERCDPRASCGHRSS
                     CRKMRPRSC"
     gene            2109..2696
                     /locus_tag="PLY56_01905"
     CDS             2109..2696
                     /locus_tag="PLY56_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20263.1"
                     /translation="MAGTRAADYVREGESRWGIHRRPSVRGRARWYSLPLIAPPPVIH
                     PLIAFERACAAINPRGILCDANLVGIYPHEGVDPALLAACLVSSLGVMMRELVGIANL
                     GQGALKTNPAYLARMLVPDLRAASSRELAALGSAFQAARQAAFPSLLDGEPDPVWRAL
                     DDAVLALLGPGAPDGARLRAAACELVSARLAKARA"
     gene            complement(2699..3100)
                     /locus_tag="PLY56_01910"
     CDS             complement(2699..3100)
                     /locus_tag="PLY56_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ20264.1"
                     /translation="MAETVPVALPDPLLTVSMLPGYCNGRLTTVSLAGELDRSNEREA
                     RSAIERALGQGSVTLIIDLGRLAFMDSSGVSLLLHAYHALMKRHGKLVLVAPPGPVRR
                     VLEMVRLSPMVPIYTTLAQAVAETSAESPDA"
     gene            complement(3779..4567)
                     /locus_tag="PLY56_01915"
     CDS             complement(3779..4567)
                     /locus_tag="PLY56_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20265.1"
                     /translation="MRSRLICRTAARQAPREHSGFTLIELLTVIAIIAILAAILLPVF
                     GKARAMARASNCLSNMRQLAQAVTMYARDYGDRLPSAFDGPLGYKKASGSGGWMYYEP
                     DSSVEILGRKFVPKWGSIWDYSGQKEAIYVCPEDELGLQSGLSYAINWQLCNQMVGSG
                     TIGSEGILYRGVKLARVKDPATVFLFVEEGNVQESGGQDVIFDSTDDGYFNGTPASWQ
                     NNPPTVRHQRGFNIVFLDGHAERLQHGSEEADKILYHTPLTGSN"
     gene            complement(4712..8533)
                     /locus_tag="PLY56_01920"
     CDS             complement(4712..8533)
                     /locus_tag="PLY56_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20266.1"
                     /translation="MHRRWRVTAALYGLALLLGVGLPVLAAQQAETAMVIESEHLRYA
                     VGNDANIVSFVDRATGTDYRASGAAACARVCKAGREYPARAASWNAGRLTLGFGDAGV
                     TAVLKVTASKHAITLEVDSVEGEGIEWLLFADIPLSLKGQPEEAFAACALALNLKTRV
                     PDLPRPASRLRAFCYPRTGLSGAAVALVAAPPDRLRAALQEVVRAAPELPKSPIGGPW
                     ALDSPDNRGSYLFNFTDLTEETVDDWIRLAKTLGITQIDFHGGASFRFGDCQPNPRLY
                     PRGVASLKAVIDRLHAAGIKAGLHTYAMFIDKRCPWVTPVPHPDLGKDATFTLAADIP
                     ADATAVPVVESTEQMSAITGFGVRNSVTLQIGDELITYTGVRKEAPFQFTGCKRGAYG
                     TRVSAHPKGAKVHHLRECFGLFVPDGDSRLFEEVAARTAQVYNDAGFDMIYLDALDGG
                     DAVAGREWAWHYEAKFTWEVWKRLKRPAIMEMSTFHHHLWYVRSRGGAWDHPNRSHKK
                     FIDIHVASNQAFNRMFLPAHLGWWAFKTWTGASGDPTYPDDIEYLCAKALGTDTGFSI
                     MGVDPNTIRKPGALPRLSAIVRRYEMLRHAGYFNEAVKEKLRVPSDEYRLVQARDGEW
                     GFLPVHYDRHKVQGLDGWSNVWKVNNRFAEQPLKLRIQGLLSAGPYDAPGNPTLATFS
                     APDEFDGPRAQSGVSLMLQPSREQVKAGGISGYLTATSTLSTPMRAWACARRVFQPPL
                     DLSGHQGLGVWVHGDGKGEVINLQLTSPPHLSRAFADHYIDVNFTGWRYFELIEPEGA
                     RHADYTWPYGGIYHIYRETVRPSSIETLSVYVNNLPPNEKMTTYLSPVRALPLVKAKL
                     RNPAVSVNGKMLTFPVEIESGQVLEFFSPAECRLYGPSGELIREVTPVGEIPALKPGE
                     NEIRFTCESPEGVNPRAYVWTITEGELVCGANPPEKVEWRYLDREEEEPVTLRASGAA
                     PPAWEVTSRAYPGPAQLEAEITVDPLASPEELYRAPDAISLESFETLDAFAETPNNRY
                     LQYVVSGPLKGVSAPPGVTHQLELSKEVVKEGAQSVRYTALSPTPGGWSARGKRFAKP
                     VDLSRCTDIGFLIHGDGAGETFYIQLRDTSGAHFDMKTVVDFTGWKYLQFPLAGAGLD
                     LSKIEYLILYYNNLPAGRRVTCYIDDIRGLRDSSVLRHPWLSVEGRRVRFPVTLSPGE
                     RLEVAGRACRVYAADGSLREEVKLKEALPRLKPGPNRVAFDADAGKTHAYQVRVQLAK
                     VYPQAADRAGQ"
BASE COUNT         1366 a         3041 c         2843 g         1495 t
ORIGIN      
        1 ccggtggcgc aagagcccgc caccgcagca gcaatcggca ggcaagccaa ccacgccatc
       61 gccagcaaag gcgccggctg ccggcgtgcc ctccggcacc tctccgaacc caccagccac
      121 cggctctccc atgccggtag ccccggcggc ttctcatgcc cggaaggacg cgggaccctc
      181 gcgatgagcg caccgcccca ctcctgcgat gcgtttctgg cccgcttcct ggatgccgcg
      241 tctgcgctgg ccgaggccct gcctgacaag gggggggacc cggagcgact tcgggaagag
      301 gctgccgaca ggctccttgc cggattcttc ctccgcttcg cgcgcgcggc atcctgcggg
      361 cttcaaccgg agctggcccg gttggcaccg tgcgccggcg ataccccgga atcgtccgca
      421 gcgggggccg cggagtctct cctcgatgcc ctcctgaacg ggtaccgcct ccaacttacg
      481 gagccgaggc ccgaccagga cgcgctcgcg ggtggggcac tcgcgccgga catcctggcg
      541 gcggcgcacg cactcacgcg cgccggccgg caggatgctg gggtcttcta cactcccgta
      601 ggcgaggtgc gcctgatgtg ccgtgaggcg ttgcgcgccc gcatggccca cgcgcttggc
      661 gtcgccgacg acgacccgga gctgctcggc attctctacg gccaaggatc ccccgatctt
      721 ccggcggaga gggccgcgct tctcttccgc gagctcgcgc gcctgcgggt ctgcgacccg
      781 gcagccgggt gtggcgcgct tctcgtcggg atggcgcatg agctctcgcg ccttctcacc
      841 tcactcggcc gtctcgtcct gccggaggac cgcaatcgct tgctggagga gggcttgccc
      901 gatcccctct cgccgacatc gatccggcgc catatcgccg cgtccacgct cttcggtctc
      961 gacctggatg cgggcgcgct gaatgttacc cgacagcggc ttttcctctt cactctcgcg
     1021 gccggggggg agcaggtgcc agatgtccgg ctcctggaag ccctgcgcgc gcatctccag
     1081 gagggagacg ggctggagcc ccctggccag acgagtggct cccaggggca cgatccgctg
     1141 cccacgaaga tcgcgggatc cgcacccacg cgcagcccgt ggctcgaccg cctcccggag
     1201 atcttcgcgc cgcccggggg cggtttcgat atcgttctga gcaacccgcc ctacgtgcgc
     1261 caagagcgtc tcgatgccag gcgcaagagt gacgcgcccc gagcctgcca ggcactccta
     1321 cccacggagt tccgctttga tgggaagagc gacctctatg cctacttctt tcacctgggg
     1381 ctcggcctct tgcgtgaggg gggcgcgctc tgcgtcatcg cgtctaactc ctggctcgat
     1441 gtcggcttcg gccaggcgct acgccgcctg ctcctgcgcc acagccgtat cctctcgatc
     1501 acggagagcg cccgaaaggg caccttcacc gatgcccgca tccacccggc gattctcctg
     1561 gcacgccggt gcatcccggc ggcggacgaa cctgagccgg tccggttcat ccgcatgcat
     1621 cacgccctgg gggagcctcg ctcgttggat gagatgaccc tcctggctgg cacccgccgg
     1681 ttcctctcga cgcccgcctt ccgggtggtt ccggtgccgc aagccgtgct cgcggcggaa
     1741 gccgtggcag acaggatggg agtgaattgg ggagggcgct acctccgcgc cccggacctc
     1801 ttcttcgaga tgctggaggc gtgcggcacg cgcctctgcc gtctggatgc ccttgcctcc
     1861 gttcgcttcg ggatcaagac gggcgctaac gacttcttct acctggagag cattccgggg
     1921 gcgcgccttc tcccgccggc gcgtccgatg acctcggggt gtttcgcaac ggcatggggg
     1981 aggagcggtt cctggagagg cgcttcctcc ggcgagcgct gcgatccccg cgcgagctgc
     2041 ggacaccgga gctcctgccg gaagatgcgg cctcgttcct gctgacctgc ccggaagagc
     2101 cggaggcgtt ggcgggtacc cgggccgccg attacgtgcg ggagggggag agccggtggg
     2161 gtatccaccg ccggcccagc gtccgcggtc gcgcgcggtg gtattcgctt cccttgattg
     2221 ccccaccgcc tgtgatccac cccttgatcg cgttcgagcg cgcctgtgcc gccatcaacc
     2281 cgaggggtat cctatgcgat gccaatctgg tggggatcta tccgcatgaa ggcgtggatc
     2341 ccgcccttct ggccgcgtgt ctggtcagca gcctgggggt aatgatgcgc gagctcgtgg
     2401 ggattgccaa cctgggacag ggcgcgctga agacaaatcc tgcctacctg gcacgcatgc
     2461 ttgtgccgga cctccgcgcg gcatcctcgc gggaactcgc ggcactcggc agcgccttcc
     2521 aggcagcccg ccaggctgcc ttcccttcgc tgctcgacgg cgagcccgat ccagtctggc
     2581 gcgctctcga cgacgcggtc ttggcgctcc tcggtccggg cgcgcccgat ggcgcccggc
     2641 ttcgcgcggc ggcttgcgag ctggtgagcg cgcgcctggc gaaagcacgc gcataggatc
     2701 aggcgtcggg gctttccgcg ctggtctcgg ccactgcctg cgcgagcgtg gtgtagatcg
     2761 gcaccatcgg cgagaggcgt accatctcca gaacacggcg cacgggcccg ggtggcgcga
     2821 cgaggaccag cttcccgtgg cgtttcatca gggcgtggta ggcgtgcaag agcaggctaa
     2881 caccactgct atccatgaag gcaagccggc ccagatcaat gatcagagtg acgctaccct
     2941 gtccaagggc acgttctatg gccgaacggg cttccctctc attgctgcgg tccagctcac
     3001 ctgccaggga gacggtggtg agacgcccat tacaatatcc tggcaacatc gagacggtta
     3061 gcagtggatc gggtaacgca accggcaccg tttcggccat ggaacccccc ctcctcgagt
     3121 ggcatctccg ccggagtgtg gttgtcccgc cagcgagtga ggcatccgtc atcgtgactg
     3181 gctccgctct cgcggaccct acccaccacc tcggatagca cgtggcggtc tcggcgccgc
     3241 atccgcatcc gcaaccgcgc acgctcatcc atgccactcc gcgctcctcc aagaatctga
     3301 accccggggc caaatccgcc agtccgggcc gcggcgaggc ggggcagaga tgcccgccag
     3361 gcgcggagcg tccgacaccg gcggatgctc ccggggcagc aagatggaac ggacaaccgc
     3421 gctgattcta cccctgcggt gcaggcccta aacacgctgg gccgcggcga gaaaggtgcg
     3481 cgatccggac aacgccgcgc ctgtgagccc tggagtcttg cctgcgagct gatgctcctg
     3541 gtatccacca agagggtggc gccactctcc agagagcaag cggcaccgcg cgcaattgac
     3601 gcgcgcggtg ccgcgttgga aacgtctccc aggccggtgt agagtgcccg ggtcaagcta
     3661 ccccatggga cggcggagtg ctgccgctca gtggcgctcc acttctacgc gccgtttcgg
     3721 ggggccggct gtccggcccc tgatgcaagc cggtgccccc ctcttacact cgaacccgct
     3781 agttcgaccc ggtgagcggc gtgtgataga ggatcttatc tgcttcctct gagccgtgct
     3841 gcaagcgctc cgcgtggccg tccaggaaaa cgatgttgaa acctcgctga tggcggaccg
     3901 tcggcgggtt gttctgccaa ctcgcgggcg tgccgttgaa gtagccgtcg tccgtgctat
     3961 cgaagatcac gtcctggccg ccggattcct gaacgttgcc ctcctcgaca aagaggaaga
     4021 ccgtggcggg atccttcacg cgcgccagct tcacaccccg ataaaggatt ccttcgctcc
     4081 caatggtgcc cgagccgacc atctggttac acaactgcca gttgatcgcg tagctcaacc
     4141 cggattgcag accgagctcg tcctctgggc agacataaat cgcctctttc tgcccggagt
     4201 agtcccaaat cgaaccccac ttcggcacga acttgcgccc aaggatctcc accgagctgt
     4261 ccggctcgta gtacatccaa ccgccactgc ccgaggcctt cttatacccc aaggggccat
     4321 cgaacgcgct gggcaagcgg tcgccatagt cgcgcgcata catcgtcacg gcctgggcca
     4381 gctgacgcat gttactcaag cagttggagg cgcgcgccat cgcccttgcc ttgccgaaga
     4441 caggcagcag aatcgcggcg aggatcgcga ttatcgcaat caccgtcaat agctcgatca
     4501 gcgtaaatcc gctgtgttcc ctgggcgcct gccgcgccgc cgttctgcag ataagtcgtg
     4561 atctcatcat agcactcctc tggtggtgcc tgttgccagc ggcagtcact ggaacccgct
     4621 tgttctccac actgcccttt tagactcctg cgtctcgcca aaagtttcgc agctctgcac
     4681 ccgggtgccc aggtttgcat ccccccccac gctactgacc ggcccgatcc gccgcctgcg
     4741 ggtagacctt cgccagttgg acgcgtacct gataggcatg ggtcttcccc gcatccgcat
     4801 cgaaggccac gcggttcggg cccggtttca gccggggcag cgcctccttc agcttaacct
     4861 cctcccggag cgagccatcg gcggcgtaga cacgacacgc tcgacctgcc acctccaggc
     4921 gctcgccggg cgagagcgtc accgggaaac gaactcgccg gccctccacg gagagccagg
     4981 gatgacgcag tacggacgag tcgcgcagcc cccggatatc atcgatgtag caggtgaccc
     5041 gcctgcccgc cgggaggttg ttgtagtaga ggataagata ctcgatcttc gagagatcca
     5101 gcccggcgcc cgcgagcgga aactgcagat acttccaccc ggtgaagtcg accaccgtct
     5161 tcatatcgaa gtgcgcgcca gaggtgtcgc gcagctggat gtagaacgtc tcgccggcgc
     5221 catcgccgtg gatcaggaag ccgatatcgg tgcaacgcga gaggtcgacc ggtttcgcga
     5281 aacgctttcc gcgcgccgac cacccgcccg gcgtcgggct tagcgccgtg tagcgcacgc
     5341 tctgcgcccc ttctttcacc acttccttcg agagctcgag ctggtgtgtc acaccgggcg
     5401 gcgcggagac ccccttcagc gggccgctca ccacgtactg caggtagcgg ttgtttggcg
     5461 tttccgcgaa ggcatccagc gtctcaaagc tctcgagcga gatggcatcg ggcgcgcgat
     5521 agagctcctc gggcgaagcg aggggatcca ccgtgatctc cgcctccagc tgcgccggcc
     5581 ccggataggc cctgctggtc acctcccacg ccgggggcgc cgcgcccgaa gcgcgcagcg
     5641 tcaccggctc ttcttcctcg cggtccaggt agcgccactc caccttctcg ggcgggttgg
     5701 cgccgcacac cagctcgccc tcggtgatcg tccacacgta cgcccgcggg ttcacgccct
     5761 ccggcgactc gcacgtgaag cgaatctcgt tctctcccgg cttgagggcg gggatctccc
     5821 caacgggcgt cacctcacgg atcagctcgc cggacggccc gtacaacctg cactccgcgg
     5881 gcgagaagaa ctccagcacc tgcccgctct cgatctccac tgggaaggtg agcatcttgc
     5941 cgttcacgct caccgcgggg ttgcgcagct ttgccttcac aagcgggagc gcccggaccg
     6001 gcgagagata ggttgtcatc ttctcgttcg gcgggaggtt gttgacgtag accgatagcg
     6061 tctcgatgct ggacgggcgc accgtttcgc gatagatatg gtagatcccg ccatagggcc
     6121 acgtgtaatc ggcgtggcgc gcgccttccg gctcgatcag ctcgaagtag cgccatccgg
     6181 tgaagttcac atcgatgtag tggtcggcga aggcgcgtga gagatgcggc gggctggtga
     6241 gttgcaggtt gatcacctcg cccttcccat cgccatgcac ccaaacgccc aggccctgat
     6301 gccccgagag atcgagcggc ggctggaaga cgcgccgcgc gcacgcccag gcgcgcatgg
     6361 gcgtggagag cgtgctcgtt gcggtgaggt acccgctgat cccgcccgcc ttcacctgct
     6421 ctcgggaggg ttggagcatc agggaaaccc cggattgtgc gcgcgggcca tcgaactcgt
     6481 ccggcgcgct gaaagtcgcg agcgtcggat tgcccggcgc gtcgtacggt ccggcggaga
     6541 ggagcccctg gattcgcagc ttcagcggct gctccgcgaa gcggttgttc accttccaga
     6601 cgttgctcca tccatccagg ccctgcacct tgtgccggtc gtagtgcacc ggcaggaagc
     6661 cccactcgcc gtcccgggcc tggaccaggc gatactcgtc gctcggcaca cgcagcttct
     6721 ccttcaccgc ctcgttgaag tagccggcat ggcgcagcat ctcatagcgg cggacaatcg
     6781 ccgagagccg gggcagcgcc cccggcttgc ggatggtgtt cgggtccacg cccatgatcg
     6841 agaagccggt gtcggtgcca agcgccttgg cgcagaggta ttcgatgtca tccggatagg
     6901 tggggtcgcc gctggcgcct gtccacgtct tgaacgccca ccacccgaga tgggccggga
     6961 ggaacatgcg gttgaaggcc tggttcgagg cgacgtggat atcgatgaac ttcttgtgac
     7021 tgcggttcgg gtgatcccac gcgccgccgc gagagcgcac gtaccacagg tggtgatgga
     7081 acgtgctcat ctccatgatc gccgggcgtt tcagccgctt ccaaacctcc caggtgaact
     7141 tcgcctcgta gtgccatgcc cactcgcgcc cggcgacggc gtcgccccca tccagggcgt
     7201 cgaggtagat catgtcgaac ccggcgtcgt tgtacacctg cgcggtgcgc gcggccacct
     7261 cctcgaagag ccgcgagtcg ccatccggca cgaacagccc aaagcactcg cgcaggtgat
     7321 gcaccttcgc gcccttgggg tgcgccgaca cacgcgtgcc atacgccccg cgcttacacc
     7381 cggtgaactg gaacggcgcc tcctttctga caccggtgta ggtgatcagc tcgtcaccaa
     7441 tctggagcgt gacgctgttg cgcactccga agccggtgat cgccgacatc tgctcggtgc
     7501 tttcgaccac gggaacggcg gtggcatcgg cggggatgtc cgcggcgaga gtgaaggtgg
     7561 catccttgcc gagatccgga tggggcacgg gcgtcaccca cgggcagcgt ttgtcgatga
     7621 acatcgcgta ggtgtggagc ccggccttga tacccgcggc gtggaggcgg tcgatcaccg
     7681 ccttcaggct ggcgacgccg cgcgggtaga gccgtggatt gggctggcag tcgccgaagc
     7741 ggaaggacgc gccgccgtgg aagtcgatct gagtgatccc cagggttttc gccaggcgga
     7801 tccagtcatc caccgtctcc tcggtgaggt cggtgaagtt gaagaggtag gagccccggt
     7861 tgtccgggct gtcgagcgcc cacggcccgc cgataggcga cttggggagc tcgggcgcgg
     7921 cgcggaccac ctcctgaagc gccgcgcgga gccggtcggg cggcgccgcg accagggcga
     7981 cagcagcccc ggaaagcccg gtgcgcgggt agcagaaggc gcgcagccgg ctggcgggac
     8041 ggggcaggtc cggcacgcgc gtcttcaagt tcagcgccag ggcgcacgcc gcgaacgcct
     8101 cttctggctg ccccttcagc gataagggaa tatcggcgaa gaggagccac tcgatcccct
     8161 cgccttccac ggagtcgacc tcgagggtga tcgcgtgctt cgaggcggtc accttgagaa
     8221 cggcggtcac gcccgcgtct ccgaagccga gggtgagccg gccggcgttc cacgacgcgg
     8281 cgcgcgcggg atactcccgg ccggccttgc acacgcgcgc gcaggccgcg gcgccgctcg
     8341 cgcggtagtc tgtgccggtc gcccggtcga cgaagctcac gatgttcgcg tcgttgccga
     8401 cggcgtagcg caggtgctcg ctctcgatca ccatggccgt ttccgcttgc tgggccgcca
     8461 ggactggcaa gccaacgcca aggagcaacg ccagcccgta gagggctgcg gtcacacgcc
     8521 atctccggtg catcgcgctc ccccttcaca ccgcgaaccc accggccgac gcgagcccgg
     8581 ccagtggatt acgccgcctg ggcgccggcg ctccacgtgc acggcaccag gcagtcctgg
     8641 tcgctgtcag tgccgtgcga cccgccggtc accttgccat cggggccgag aacatcgtgc
     8701 tggccgtggt cggccaggag gatcactccg tattgaagcg ccgtg
//
LOCUS       DAOSCV010000042         8610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5359_length_8610_cov_3.647575, whole genome shotgun sequence.
ACCESSION   DAOSCV010000042 DAOSCV010000000
VERSION     DAOSCV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_8610_cov_3.647575"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLY56_01925"
     CDS             <1..401
                     /locus_tag="PLY56_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOJ20267.1"
                     /translation="EYEAAAGSYSIKRAMLDKAKKDALIMHPLPRVDELDYRIDRDRR
                     AAYFRQAGNGVPIRMALSALLLGAEDPGPGTHPPETHATPVNTPPGLVCPNERCVTRN
                     EPYLTPRFVSVAGHEGALQCAYCDREVQQP"
     gene            complement(444..1142)
                     /locus_tag="PLY56_01930"
     CDS             complement(444..1142)
                     /locus_tag="PLY56_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOJ20268.1"
                     /translation="MEQLLSSMTRAVEGSPGIALGAAALWGVLSVLLSPCHLAGIPLI
                     VGFIGNQATMSARRAFALSLAFAGGILITIAGIGAITASLGRMAGDVGAAGNYVVAAL
                     FFVVGLHLMDVIPLSIPAVGPGKIRHRGILAALLLGLVFGIALGPCTFAYMAPVLAVT
                     FKASATQPLYGASLLIAYGAGHCAVIVAAGASTEVVQRYLHWSRESRGTALLKKSCGF
                     LVLLGGLYLLHTAR"
     gene            1416..1553
                     /locus_tag="PLY56_01935"
     CDS             1416..1553
                     /locus_tag="PLY56_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20269.1"
                     /translation="MTVKSGDPVLLEQLEECRQRRFDRNGRKMYYPEQWGDADFEPLL
                     K"
     gene            1589..1828
                     /locus_tag="PLY56_01940"
     CDS             1589..1828
                     /locus_tag="PLY56_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20270.1"
                     /translation="MTSDELLMQEDVKNLMEQYGFSREQAERAVQTNLRAGSDVGIKV
                     DGKFIPVYSPITAVRDEVLKEAVRLQREQYGVAAI"
     gene            complement(2166..2594)
                     /locus_tag="PLY56_01945"
     CDS             complement(2166..2594)
                     /locus_tag="PLY56_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOJ20271.1"
                     /translation="MHPWRGHAGMMHKMAWLHRWEHLDLSDAQKEQLRQILFNTKKEM
                     IRRWAEVRVAKLELKQMLMEDAPDRMLVEQAVRNIGQKKTEMGLLMANAKLDALAVLT
                     PEQRRELHEISPHTECPEYGHKGGLAGALGEEDEEEMGEA"
     gene            complement(2946..4025)
                     /gene="rsgA"
                     /locus_tag="PLY56_01950"
     CDS             complement(2946..4025)
                     /gene="rsgA"
                     /locus_tag="PLY56_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017719954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ20272.1"
                     /translation="MRLDALGWSGFYAGHFAAQAGEDHVPGRVIAEHKGQYRVQTEAG
                     EMAAILPGKVLHAATRRDELPVVGDWVILQILPEEPPKALVHGILPRRSWLSRKEAGT
                     KPGEQPVAANIDTLFLAVGLDDNYRLRRIERYLAVAWQSGADPVVLLTKADLCSQLEE
                     RLAEARAVAGIAPIHAVSALTGEGLDALAQYLVPRRTLALLGSSGVGKSTLINHLLGE
                     EVQSTQPVRAGDSKGRHTTTSRQIFLLPSGALEVDTPGIRELQIGDAWEGVAEAFPEI
                     EELAFGCRYADCQHEREPGCRVRAALSDGTLDPARFENYRRMRRELAYQESTRDRKAE
                     RERKQQAKKLCKMIARLPKKGESGR"
     gene            complement(4133..4684)
                     /locus_tag="PLY56_01955"
     CDS             complement(4133..4684)
                     /locus_tag="PLY56_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ20273.1"
                     /translation="MNSLFERASAGDAHATRMVVDSLRPRLAKMAAYYARRTGEEADD
                     LLQEAWLGLLEALPGLDVRIGSPEQYLIHQARWRLLDAVRRASTRRFASLDDSALEIE
                     AREESPAGYACVAEFSRRLKPVQRQVLSLLVDGFTWREAGGTLGCTSANIAYHVRQIR
                     RCYEEWLGEAGPDAPCISQPKRR"
     gene            complement(4986..6029)
                     /gene="add"
                     /locus_tag="PLY56_01960"
     CDS             complement(4986..6029)
                     /gene="add"
                     /locus_tag="PLY56_01960"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOJ20274.1"
                     /translation="MNRVAQSVSPFLQQLPKVELHLHLEGAIPPETLLGVFRRHGNTE
                     MACLEDVLRLYQHRDFYDFLAHFRTILEWLRDADDLEPITYDVLSNLARQNVRYAEVI
                     FSPGTLLHFHGLDPDRVLDAIQRGYARARSEHAIEMALLLDLTRNMGLEYGWRMARFA
                     AGAQDRGVIGITLGGNERDFPAAPYAEIYQWARDQGLRVTAHAGEAAGPESVWDCIRL
                     LGVERIGHGVRMEDDPRLAEYVVEHGIAIECCPWSNVLTGAVESLEAHPVRRWFERGV
                     RLSINSDDPTLFGTDITRELARLQAAHGFSDGEILHIMAQAVQMSFLPEGGKARLRRL
                     LAEFAVQNGFQTL"
     gene            complement(6026..6694)
                     /locus_tag="PLY56_01965"
     CDS             complement(6026..6694)
                     /locus_tag="PLY56_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ20275.1"
                     /translation="MKEEFHEERRAVARCMRRVYRLRLTSATGGNISCRAGADRFAIT
                     PSGIDKARIRAREVGLIDLEGRNLTPELKPSCEWLMHLRIYQRYPSVGAIVHAHPVTL
                     SAFSCAETPIDIGLLSETYALLDPPVVAPYALMGTEELAEIVAECAGRSSAILLRNHA
                     VVTTGANLMQAFSRLEVLEEAARVTLITRQLEGVRGLSPEQRAELDRFMGRDVRPAKT
                     DELP"
     gene            6969..8396
                     /locus_tag="PLY56_01970"
     CDS             6969..8396
                     /locus_tag="PLY56_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HOJ20276.1"
                     /translation="MERPDVRIERDTLGEKEVPADAYYGVQTARAMENFPVSGHRAHP
                     ALIAALGTIKRAAAEVNADLGLLDRELAEAISEAAQEVADHVWDDQFVVDVFQAGAGT
                     STHMNANEVIANRANELLGSTKGSYRPVHPNDHVNMGQSTNDVIPTAIRLAALAVLPD
                     LLRALESLEEALRMKAEEFDGVLKAGRTHLQDAVPVRLGQEFGAYATAIAHDRERLVT
                     AGESLRRIGIGGTATGTGLNTHPEYHSRMVARLSQLTGESLRSFGDLFAAMQSMADPL
                     EASAATRTLAVTLTRIANDIRLMTSGPTTGLDEIRLPALQPGSSIMPGKVNPVMAEML
                     NMVAFHVIGSDLAVALAAQAGQLELNVMMPLIAYELLYSQSYLARAVQLFADRAVRGI
                     AANEAKCRSWMEGSLGLATALNPYIGYHAAAEVAKEALLTGKSVPEIVRERHLLPPEE
                     LEKVLSPAAMTEPNLPGGGTSHDTA"
BASE COUNT         1513 a         2909 c         2764 g         1424 t
ORIGIN      
        1 ccgagtatga agcggctgcc ggcagctact ccatcaagag ggcgatgctc gacaaagcga
       61 aaaaggacgc cctcatcatg cacccgctcc cccgggtgga tgagctggat taccgcatcg
      121 accgcgaccg ccgggcggcc tatttccgcc aggcaggcaa cggcgtgccg atccgcatgg
      181 cgctctccgc gctactcctt ggcgccgagg atcctggtcc cggcacgcat cccccggaga
      241 cgcacgccac tccggtgaac actccgccgg gcctggtctg ccccaacgaa cgctgcgtca
      301 cccggaacga gccctacctc accccgcggt ttgtcagcgt tgccgggcac gagggggcgc
      361 tccaatgcgc ctactgcgat cgcgaggtgc agcagccgta gtgccggcgg caacggacag
      421 gacgatccac ctccgctcgg ctcctaccgc gcggtgtgga gcaggtagag cccgcccagc
      481 agcacgagga agccacagct cttcttgagg agcgccgttc cgcgggattc gcggctccaa
      541 tggaggtagc gctgcacgac ttcggtggac gcgccggcgg ccacgatgac ggcgcagtgg
      601 ccggccccat aggcgatgag caggctggcc ccgtagagcg gctgcgtggc gctcgccttg
      661 aacgtcaccg cgagcacggg cgccatgtag gcgaaggtgc aaggccccag ggcaatccca
      721 aaaaccagcc cgaggagcag ggcggccaga attccccggt ggcgtatctt accggggcca
      781 acggctggga tggagagggg gatcacatcc atcaggtgca ggcccaccac gaagaaaagc
      841 gcggccacca cgtagttgcc ggcggcgccc acgtcgccgg ccatgcgtcc cagtgaggcg
      901 gtgatcgcgc caattccggc gatggtgatc aggattcccc ccgcgaaggc cagggagagt
      961 gcgaatgcgc gccgcgcgct catcgtcgcc tggttcccga tgaagcccac gatcagcggg
     1021 atgccggcca gatggcacgg gctgagtaga acgctcaaca ccccccagag ggcagcggca
     1081 ccgagcgcga tgcccggcga tccctccacg gcgcgggtca tcgaactgag gagctgttcc
     1141 atgcttcaga agccctccac ccatctcatc gcggagcaca ccgggcgctc cgcgacgccc
     1201 tactccttta gaccccgctg aaggcaattg cgcgcccaga agtttcttga tctccgcagg
     1261 gaagagcacg cgcccggatg acttcgcttt gccatccact gccagggcca gtgtcgccat
     1321 cccccccgag cttcaccata tcatagcata cggatagagc tgcctgggcg ctgtggcggc
     1381 actctgacat cttcgcccgg gcgtatgccc agtatgtgac cgtgaagagc ggcgaccccg
     1441 tgctcctgga gcagctggag gagtgccggc agaggcgttt tgacagaaac ggccggaaga
     1501 tgtattatcc cgaacaatgg ggtgatgccg acttcgagcc gctgctgaag tagttcgatg
     1561 acgagtttgc ccggagggag gggatcctgt gacaagcgac gagctgctga tgcaggagga
     1621 cgtgaagaac ctgatggagc agtatgggtt ctcgcgcgag caggcagagc gcgccgtgca
     1681 gacgaacctg cgtgccggga gcgacgtggg catcaaggtg gacgggaagt tcatcccggt
     1741 gtactcgccg atcacagcgg tgcgcgatga agtgctgaag gaagcggtga ggctgcagcg
     1801 cgagcaatat ggtgtggcag caatatagag catcctgctc tggcaggatg aacatagagg
     1861 gcagggcgaa caagggggtt cgcccctaca ggcggggcag tgcatggggc ctggcggtgg
     1921 aagccgctgg gccttgtttg ttgggggctg gtccaatcgg gccggcccct tatcgtttgg
     1981 ggcggctttc gggctgccgc gggagccggc ggcaggatct cacctgtccg ccatcatcgc
     2041 gtgggtgaga cgctgcggcg tagtagctcg taggacggca tcagcgccgg cggaacgtcg
     2101 gattgatgaa gtccggtcgc gcgcaaaacg cacgcgaccg gagaaaagcg gcaatcagga
     2161 gagggctacg cctcacccat ctcctcttca tcctcctcgc caagggcacc cgccagacca
     2221 cctttgtgcc catactccgg gcactcggtg tgcggcgaga tctcatggag ctcgcgacgc
     2281 tgttcagggg tgagcactgc gagggcatcc agcttggcgt ttgccatcag caggcccatc
     2341 tccgtcttct tttgcccgat attgcgaacc gcctgctcca cgagcatgcg atccggcgcg
     2401 tcctccatca gcatctgctt tagctcgagt ttggccacgc gaacctccgc ccaccggcgg
     2461 atcatctcct tcttcgtgtt gaataggatc tgacggagct gctctttctg ggcatcgctc
     2521 aggtccagat gctcccagcg gtggagccag gccatcttgt gcatcatgcc cgcgtgcccc
     2581 ctccatggat gcatgtgtcc gcacattcgg ccacccatca tcgccctcct tgccaccttt
     2641 ccgaacccta cgcgcatgcg aatggaggcg tccgcagatc ccagtatgga gccgttgatg
     2701 tgtgccctct tcctgatgcg tcacgcggat ggttccgccg ccacagcata cgctctttgc
     2761 caggtgggtc ccggatcggg cttacccggg cggttcctat gccaaacctg agcgtcttcc
     2821 aggcgatccc cagggagggt cccgccgtgc tttttccgcc cgggcgccca accgcgatag
     2881 cctgagcccg gcgcgcgcgc cgtgatcccg gtcccggggc aggaaagcta agaggaacgc
     2941 tcgtctcagc ggcccgattc cccctttttt ggcagccgcg cgatcatctt gcacagcttc
     3001 ttggcctgct gcttgcgctc tcgctccgcc ttccggtcgc gcgtcgactc ctggtatgcc
     3061 agctcccgac gcattcgccg gtagttctca aaccgcgccg gatccagcgt gccatcactc
     3121 agtgccgctc ggacccggca tcctggctcg cgctcgtgct ggcaatcggc atagcgacac
     3181 ccgaacgcca actcctcaat ctctgggaac gcctccgcga ccccctccca tgcgtccccg
     3241 atctgcagct cccgtatacc tggagtatcc acctcgagcg cgcccgaagg gaggagaaag
     3301 atctggcggg aggtcgtcgt atgccggccc ttgctatcgc cggcacgcac cggctgcgtt
     3361 gactgtacct cctctcccaa gaggtggttg atcagcgtcg atttccccac gccagatgac
     3421 ccaagcagcg ccaaggtgcg ccgcgggacg aggtattggg caagcgcatc caggccctcg
     3481 cccgtgagcg cgctcaccgc gtggatcggg gcgatgccag ccaccgcccg tgcctcggca
     3541 agccgctcct cgagctgcga acagagatcc gccttcgtga gcagcaccac tggatcggcg
     3601 ccgctctgcc aggcgaccgc caggtagcgc tcgatgcggc gcaggcggta gttgtcatcc
     3661 aggccaacgg ccaggaaaag cgtgtcgata ttcgcggcca ccggctgctc gccaggcttg
     3721 gtcccagcct ccttccgcga cagccagctc cgacgcggca gaatgccgtg taccagcgcc
     3781 tttggcggct cttcaggaag tatctgaagg atgacccagt cgcccacgac cggcagctcg
     3841 tctcgccgcg tggcggcatg cagcaccttg ccgggcagga tagccgccat ctccccggcc
     3901 tccgtttgca cccggtactg gcccttatgc tcggcgatca ctcgtccggg cacatgatcc
     3961 tcgcccgcct gcgccgcaaa atgcccggcg tagaacccgc tccatcctag cgcatccaac
     4021 cgcatctctc ttcccccctc tctcccctga aatcaccgcg cggtggcacc gtcgtgccaa
     4081 ccgcccgccg aagcgtcctc tcgatgacaa catactccat gcccgcccct ctctatcgtc
     4141 tcttaggctg cgaaatgcac ggagcgtccg ggccggcctc tcccagccat tcctcatagc
     4201 accggcggat ctggcgcacg tgataggcga tgttcgctga tgtgcatccc agggtgccgc
     4261 ccgcctcgcg ccaggtgaaa ccatccacca acaggctgag cacctgccgt tggaccggct
     4321 tcaatcgccg ggaaaactcc gccacgcagg catagccggc cggcgactcc tcacgggctt
     4381 cgatctcaag ggcggaatcg tcgagcgagg cgaagcggcg cgtgctcgcg cgtcggacgg
     4441 catcgagcag gcgccagcga gcctgatgga tcaggtactg ctccgggctg ccgatgcgca
     4501 cgtccaggcc gggcagcgcc tccagcaagc cgagccacgc ctcttgcagg aggtcatcgg
     4561 cctcttcgcc ggttcgacgg gcgtagtagg cagccatctt cgcgagccgc gggcgcagag
     4621 aatcgaccac catgcgggtg gcgtgcgcgt cccccgcgct cgcgcgctca aacagggaat
     4681 tcattgttgg tctctcctcg aaagcggtat cagaatgcag gcgcgcgcgc ccacctaccg
     4741 gtagcgctcc ggaagagacc cttgcccagg gagggcgaaa ggggagccca tgccttcagc
     4801 aggggctagg ccggaaggct accggatcgg gaggcgcttc gcgcgcggcg cacgatttga
     4861 ttgggtttgg tggtcaccct cattcgtacc tctcctttcc ggttatgtcg ggagcaccgg
     4921 cgtgccgcta tcggcacggc cccgaaccac tcccatattc cttatcgccg caacggctcc
     4981 tcttgctaaa gagtctgaaa tccattttga acggcgaact cggccagcag gcgccgcagg
     5041 cgggccttcc ctccctcggg caggaagctc atctgcaccg cctgcgccat gatgtggagg
     5101 atctcgccgt cgctgaagcc gtgggcggcc tgcagccgcg ccagctcacg cgtgatgtcg
     5161 gtgccaaaca gcgtcggatc gtcggagttg atcgagagcc tcacgccgcg ctcgaaccac
     5221 cggcgcaccg ggtgggcctc cagcgactcg accgcgccgg tgagcacgtt gctccagggg
     5281 caacactcga tggcgatgcc gtgctcgacg acgtactcgg ccagccgggg gtcgtcctcc
     5341 atgcgcacgc catgcccgat gcgctcgact cccagaagcc ggatgcaatc ccacacactc
     5401 tccggccccg cagcctcgcc cgcgtgcgcc gtcacacgca ggccctggtc gcgcgcccac
     5461 tgatagatct ccgcgtaggg cgcggcgggg aagtcacgct cgttgccgcc cagggtgatg
     5521 ccgatcacgc ctcgatcctg cgcgccggcg gcaaaccgcg ccatccgcca gccatactcc
     5581 aaccccatgt ttcgggtcag gtccaggagg agcgccatct caatcgcgtg ctcgctccgg
     5641 gcgcgcgcat agccgcgctg gatcgcatcc agaacccgat ccgggtccag accgtgaaag
     5701 tggagcagcg tgccgggaga gaagatcact tcggcatagc ggacgttctg ccgcgccagg
     5761 ttggagagga cgtcgtaggt aatcggctcc aggtcatcgg catcgcgcag ccactccagg
     5821 atcgtgcgga agtgcgccag gaagtcgtag aagtcgcggt gctggtagag gcgcaacacg
     5881 tcctccaggc aggccatctc ggtattgcca tgccggcgga acacgccaag gagcgtttcg
     5941 ggcggaatgg cgccctccag atggaggtgc agctccacct tgggcagctg ctggaggaag
     6001 ggactcacgc tctgggcaac acgattcatg gaagctcgtc cgtcttcgcc gggcggacgt
     6061 cgcgccccat gaaacggtcc aactcggcgc gctgctctgg cgagagccca cggaccccct
     6121 cgagctggcg ggtgatcagc gtcacccggg ccgcctcttc cagtacctcc agccggctga
     6181 acgcctgcat cagattcgcc cccgtggtga ccaccgcgtg gttgcgcagg aggatcgcgg
     6241 acgagcgccc ggcgcactcc gcgacgatct ccgccaactc ctcggtaccc atcagggcgt
     6301 agggagcgac caccggcgga tcgagaaggg cgtacgtctc cgagaggagc ccgatatcga
     6361 tcggggtctc ggcgcaggag aaggcgctga gggtaacggg gtgggcgtgg acgatggcgc
     6421 caaccgacgg gtaacgctgg taaatccgca ggtgcatcag ccactcgcag ctgggcttca
     6481 gctcgggggt gaggttccgg ccttcgaggt cgatgagccc cacttcacgc gcccggatgc
     6541 gcgccttatc gatgccgctg ggagtgatag caaaccggtc cgcgcccgcg cgacacgaga
     6601 tatttccgcc cgtcgcgctg gtcagccgca agcggtaaac gcgccgcatg caacgggcga
     6661 ccgctcgccg ctcctcatga aactcctctt tcatctgatt gcctctcgct gccgtagcta
     6721 ggcgaatgct cgcccgactc tactctctct ttggcgtttt cgccccatct tcccttgcgg
     6781 cgccgattcc cataggcgcg cgcggatcag gccctttgga gcgaggtacc ctgtgggaga
     6841 ggattcgacg cctctgggga aggttccgac ggcgactcgc accccggctc aggatggagt
     6901 ggcacctttt ctgcgccggg cccggcagga gccgagggct ccgccatgca gtgcgggagg
     6961 gagactggat ggagaggccg gatgtgcgga tcgagcgaga tacgctgggc gagaaagagg
     7021 tgcccgcgga tgcctattac ggggtgcaga cagcgcgcgc gatggagaat ttccccgtca
     7081 gcggccaccg cgctcacccg gcgctcatcg ccgccctcgg cacgatcaag cgtgccgcgg
     7141 ctgaagtaaa cgccgatctt ggattgctgg atcgcgagct ggccgaagcg atcagtgagg
     7201 cggcacagga ggtcgccgat cacgtctggg acgatcagtt tgtcgtagac gtgttccaag
     7261 ccggggccgg cacctcgacg cacatgaacg ccaacgaggt gatcgccaac cgcgccaacg
     7321 agcttctggg cagtaccaag gggagctacc ggcccgtcca ccccaacgac cacgtgaaca
     7381 tggggcagtc gaccaacgac gtgatcccga cggcgatccg gctggcggcg ctcgcagtgc
     7441 ttccggatct cctccgcgcc ctcgaatcgc tggaggaggc gctgcgcatg aaggccgaag
     7501 agttcgacgg cgtgctcaag gccgggcgca ctcacctgca agatgccgtt cccgtccgcc
     7561 tcggccagga gttcggcgcc tatgccaccg ccatcgccca cgatcgcgag cgcctcgtca
     7621 cggcgggaga gagcctccgc cgcatcggga ttgggggcac cgccacagga actggcctca
     7681 acacccatcc ggagtaccac tcgcgcatgg tggcccggct ctcacaactg acgggcgagt
     7741 cgctgcgctc ctttggcgat cttttcgcgg cgatgcagag catggcggat ccgctggagg
     7801 cctcggcggc gacacgcacg ctggcggtca cgctgacgcg catcgccaac gacatccgcc
     7861 tgatgacttc agggccgacg acggggctgg atgagatccg gctgccagcg ctgcagcccg
     7921 gttcctcgat catgccgggc aaggtcaacc ctgtcatggc agagatgttg aacatggtgg
     7981 cgttccacgt gatcggcagc gacctggcgg tagcactcgc ggcccaggca ggccagctag
     8041 agctgaatgt gatgatgccg ctcatcgctt acgagctgct ctactcgcaa agctacctgg
     8101 cgcgagcggt gcagcttttt gccgaccggg ccgtgcgcgg gatcgccgcc aacgaggcaa
     8161 agtgccggag ctggatggag ggaagtctcg ggctggcgac ggcgctgaac ccctacattg
     8221 ggtaccacgc ggccgcggag gtcgccaaag aggctctgct caccggcaag agcgttccag
     8281 agatcgtacg cgagcgccac ctgctcccac ccgaggagct ggagaaggtg ctctcgccag
     8341 cagcgatgac cgagcccaat ctccctgggg gtggaaccag ccacgacacg gcatagttca
     8401 tgcacgcttg tctggcacac ccacggcgtt ccccgctcgc ggaacaggga tgcgccgaaa
     8461 aaagggggaa agaacaatga agctaacgca tatcgtgtgg gcctgcctgg tgctggccct
     8521 cgcgggatgt gggggagggg gctcggacgc cggcacttca ccgccgacgc acgcccccga
     8581 ggtagtgcaa ccggtcgagg tgccgcagac
//
LOCUS       DAOSCV010000043         8590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5373_length_8590_cov_3.265612, whole genome shotgun sequence.
ACCESSION   DAOSCV010000043 DAOSCV010000000
VERSION     DAOSCV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_8590_cov_3.265612"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="pyrR"
                     /locus_tag="PLY56_01975"
     CDS             complement(<1..403)
                     /gene="pyrR"
                     /locus_tag="PLY56_01975"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOJ20277.1"
                     /translation="MDEQDIRRALTRIAHEIIEKNRGAERLALVGIRTKGAPLAARLA
                     TLIQRFEGVPVPVGELDVAAHRDDDRRAATDTSDIQFEVTGRIIVLVDEVFYTGRTVR
                     AALDALMDRGRPDAVQLAVLVDRGHRELPIRP"
     gene            complement(839..1444)
                     /locus_tag="PLY56_01980"
     CDS             complement(839..1444)
                     /locus_tag="PLY56_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20278.1"
                     /translation="MMARKGFTLIELLVVIAIIAILAAILFPVFARARENSKRTSCLS
                     NLKQLAQGALMYAQDYDERLPVNSTQGNPLLPVVQALWPYVKNRTIFYCPSIQVLSVA
                     NPLLNNTDDNWNAGNIGYYFWSSWGFHEHTGPFLDRHPPRQLTLSHDPDLWMWSDVFG
                     AFYWQQNIPFSHNMPKWSFTNVAFMDGHVKSVSGRPVEIFK"
     gene            complement(1726..3708)
                     /locus_tag="PLY56_01985"
     CDS             complement(1726..3708)
                     /locus_tag="PLY56_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ20279.1"
                     /translation="MVQRKRVGEATEWDLIGEGAGEMFHRFCHAWSARISPENPLPAT
                     PEEWPAIRDARRARLREAMGLSLAEPCPLRPEVVAVEDRGDYTLERVTFHSRPNLVMT
                     THLYVPKEAPKPAPAILSVHGHWSQGKLAPRVQERNIGMAKLGFVVLCVDAIGSGERA
                     YPGSEYHGRQLGAQALPTGLTLPGLQVWDNMRALDYLASRPEVDPSRIACTGASGGCN
                     QTLYLAALDERVAAAAPVCCIGSLEGYLNGPHCVCELVPGIKAIGEIGDVLALIAPRP
                     LILINGIHDTGAGFQIDDMRAQFPKLKQLYELLGAGEKVTKCEIDLGHGYFPAMREVM
                     YAWFRRWLMGIQADLCPEPEVQPAEPEQICCFPGGKMPAEYDTVPSLAFAEAQRLAQS
                     YPQPEEIARNSGVRASLRARLTEVLALPEESQPAIETTRSFPGDREGVVITTEPGIRV
                     PMVIAQAKGATSCVLWLDTAGKASILSEGLPSVDGNQAIAIPDLRGLGETHWERGYTV
                     GVWDYQYFQNGTILGRPMLGMWVWDTLQCARVLRERFDSVSVAGKGPLAIVALLAAAL
                     DEGITSARCEEILASYIYPEGFSREHAMSLFVPSLLKVADLPQIAGLVAPRRLQIASA
                     VDGGARPLSPAELSSSFDLTRQVYQHAGAEGALVLE"
     gene            complement(3776..4504)
                     /locus_tag="PLY56_01990"
     CDS             complement(3776..4504)
                     /locus_tag="PLY56_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOJ20280.1"
                     /translation="MMQRYLLHIMTLLLLAPAARADDRGILAHWTMDEVSDGIVRDAT
                     GRGHDATFQAAGTAKPVLEPGIIGKALRLREGEQAFLSVANFADLMPPKGLTAMAWIK
                     PSARNKTYEILCSKGDKAGQPPWPGWRLRFHWTRAQFQYGTADGKEPRVESASWLVPA
                     GFWSHIAVVHDGTWMRLYINGVEKAAAPAEGEIMPSKRGLIIGNYIGRKNAYAFDGLL
                     DDVKVFGRALTAEEVFTEAVRGME"
     gene            complement(4604..7528)
                     /locus_tag="PLY56_01995"
     CDS             complement(4604..7528)
                     /locus_tag="PLY56_01995"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20281.1"
                     /translation="MRVAMIKRFLYLLLAMVPVAIWAGEAPKPPKVVATRAAVAPKID
                     GRLDDAAWQAATWYGGFTLLDQVTVQANPQTRFKVLFDDSRLYFGIECAEPAIKDLHA
                     TVREHDGPVHRDDCVEIMLDPTGERVEYYHFTINPLGTRYDAELRQGGHVRSKEWNCD
                     WVAQAHIGEQSWQVEAAIPFVELGLTGKSTGAWALNVTRERQAGQTQLSSFAPTTGGF
                     HQPALYASLELPGATLSRYFWKMERPFEASVAREEGRLLYRAKLHLMNETGRLLPIEL
                     VPTLVQAGKSLAGPPVQDMLDAGQGREYSFKAPLPGEGNQTLVLTVRDRRDPNRVYCV
                     RSLPVTLDYVPAEIIVTRPAYRNSIYATEKIDAVEATLRLALSPEEMAGARALVSLVP
                     EGQAGGKPVATTRVDKLEAQTRLRLPIGALAEGSYLLQVAVQERSGKQICVATAPIRK
                     LPPAPNGHEWRLDEQMVLRHNGEPYLPFGWFSIPPAEMAKSDCPYTAMQTYNTQYQSV
                     EQARAFLDEVAAAKTYVTLYPYPSSKMMNAWGEPLSDDDAKALAARIDALKDHPGILA
                     WYMADEPELRPALPERTRRLYQVVADADPYHPCIMLNDTIAGIHKYADGGDILMPDPY
                     PCFLKGGYAAQPIEKVSAFMKAAREASGGRKAIWITPQGFNYGDYGRAGQRGPNLTEL
                     RNMTYQAVVYGATGFLWYTFSHAGNYPDIGLGMPFLAREVQRLKAFVLSPDVPDAVRA
                     NAPKPEHIHVSLRRAGGQSVLFVVNTATETQSVELTLPQEMARELHVVSEGRTVAARD
                     GRLRETFTPYAVHIYATSAELAGQERLADLQRAIDAANAARKKPGNLAFEDSGVTVSY
                     SSESKYGCTPERVVDGVTTNMLWQDGTPNQFPDWIALTWPAAQRIGRVVTYSPTLADF
                     EVQVPDGEGWRTVATVRNATTDAIEATFAPVETKVIRLQITRLRQGETLSRVWEIEAY
                     EK"
     gene            complement(7562..8434)
                     /locus_tag="PLY56_02000"
     CDS             complement(7562..8434)
                     /locus_tag="PLY56_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20282.1"
                     /translation="MFIDIHAHAYKKIGPKQDGRIHFVTPEQLLERYEELKIEKACLL
                     PLIGPEVYLPESNEEILEIAERYPDRFIPFCNVDPRALSNSPDAPLGDVLRYYRDQGC
                     KGIGELMPNLPFLDPRVQNLFKHAQDVGFPVIFDISTRIGGTYGLYDDQGLPQLETTL
                     QRFPRLIILGHGPAFWAEIGRLETPADRGSYPRYPIHEEGVVPKLFRRYPNLYGDLSA
                     GSGYNALARDPDYAVRFLNEFQDRLLFGTDICRADQPAPQVEFLLGLKASGKLSEEVF
                     RKIARENAIRLLGL"
BASE COUNT         1421 a         2746 c         2899 g         1524 t
ORIGIN      
        1 ccggacggat cggcaactcg cggtgcccgc ggtcgaccag gacggccagc tggacggcat
       61 cgggccggcc gcggtccatg agggcatcga gggcggcacg gaccgtgcgc ccggtgtaga
      121 acacctcgtc gactagaacg atgatacgcc cggtgacctc gaactggata tcggaagtgt
      181 cggtggcggc tcggcggtcg tcatcccggt gggcggcgac gtcgagttca ccgacgggaa
      241 cgggaacgcc ttcgaaccgc tggatcagcg tggcgaggcg ggcggcgagg ggggcgcctt
      301 tggtgcgtat gcctacgagg gcaaggcgct ctgcgccgcg gttcttctct ataatctcgt
      361 gcgcgatccg ggtgagcgcc cgtcgaatat cctgctcatc catcacgcgc gtgccatgcg
      421 ttggtgtcac gcagcctcct cagatccctg cacggggacc cggtacaaga aaaggagccc
      481 cctcgccttc agggcgtatg gaggctccca gatatgagtg cgcgctatcc gaccttgccg
      541 gcctcacggg accgtctcaa aggcgatgga cgtctccagc cacttgcgtg cggcccgaaa
      601 gatatcggcg aaattctagc acgatccttt tccgtgtgtc aagcagaatc tgcgccaggc
      661 gtcaggttga gattctgaga aaaggccgct ccgggtgcgt gtgacgcgca acccgacttg
      721 tctcggaatc ccaggacccc tcccctcctt cggtgctttc cacaggaagc cacgcgcctc
      781 tggcgggaga agcatctgca ttcccaccct atgatcttca cccgcacgcg agcgcgtcct
      841 acttgaagat ctccacgggc cggccgctca ccgatttcac gtggccgtcc atgaaggcga
      901 cgttggtgaa gctccacttc ggcatgttgt gcgagaaggg gatgttctgc tgccagtaga
      961 aggcgccgaa gacatcgctc cacatccaga gatccgggtc gtgcgacaga gtgagctgtc
     1021 gcgggggatg ccgatccagg aacggccccg tgtgctcgtg gaagccccag ctggaccaga
     1081 agtagtagcc gatgttaccc gcgttccagt tgtcgtcggt gttgttgagc agcggattgg
     1141 cgaccgagag cacctggatt gatgggcagt agaagatcgt cctgttcttc acgtagggcc
     1201 aaagcgcctg gacgaccggc aataacgggt tgccctgcgt cgagttgacg gggagccgct
     1261 catcgtaatc ctgggcatac atcagggccc cctgggccag ctgcttcagg ttgctcaggc
     1321 agctggtgcg ctttgagttc tcgcgcgccc gcgcgaagac aggaaagagg atcgctgcca
     1381 ggatggcgat gatcgcaata acaacgagca attcaattag agtgaaacct tttcgtgcca
     1441 tcattcgcat cgcctttcgt gcggggttca cgagtgtgtg gaccgaaagg cacctcctgt
     1501 tcctatcgtc aggctctatt ctaccatcat cgcccgcctg ccgccgtgaa gagggaatta
     1561 agcggttgtt aaatgagctt taaggcagcc gggcggagtt ctccggcacg ggcgtgggga
     1621 tgctgggggc tggcaggcgg gtgtggttca cgcgcacagc ggggcagcgc gtgcccccaa
     1681 ttcgggcgca ggcgctgccc cgcaaggctt gttatcccaa aggggctact ccaggacgag
     1741 tgccccttcg gcgccggcgt gctgatagac ctggcgcgtg aggtcaaagc tgctggagag
     1801 ctcggctggc gagagcgggc gggcgccgcc atctacagcg ctcgcgatct gcagccggcg
     1861 cggggcgacc aggccggcga tctggggcag atccgccact ttcagcaggc ttggtacgaa
     1921 gaggctcatg gcatgctcgc gcgagaagcc ttcgggatag atgtagctgg cgaggatctc
     1981 ctcgcagcgc gccgacgtga tcccctcatc cagggcagcg gcgaggaggg ccacaatcgc
     2041 cagggggccc ttccctgcta cggagacgct atcgaaacgc tcgcgcagga cgcgcgcgca
     2101 ctgcagggta tcccagaccc acattccgag catcggccgg ccaaggatgg tgccgttctg
     2161 gaagtactgg taatcccaca cgccgacggt gtacccgcgc tcccagtggg tctcgcccag
     2221 gccgcgcagg tcaggaatcg cgatcgcctg gttgccatcg actgagggca ggccctctga
     2281 gagaatgctg gctttgcccg cggtatccag ccagaggacg caggatgtgg cccccttcgc
     2341 ctgcgcgatc accatcggca cgcgaatccc cggttccgtg gtgattacca cgccttcgcg
     2401 atccccgggg aaggagcggg ttgtctcgat ggcgggctgg ctctcctcgg gaagagccag
     2461 aacctctgtc aggcgcgcgc gcaggctcgc gcggactccg gagttgcggg cgatctcttc
     2521 aggctgggga taactctggg cgaggcgctg ggcctcggcg aaggcgagcg aggggacggt
     2581 gtcgtactcc gccggcatct tcccgccggg gaagcagcag atctgctctg gctcggcagg
     2641 ctggacctcg ggctcggggc aaaggtctgc ctggattccc atcagccagc gccggaacca
     2701 ggcgtacatc acctcgcgca tggcagggaa gtagccgtgc cccaggtcga tctcgcactt
     2761 cgttaccttc tctccggcgc ccagaagctc gtagagctgc ttcagcttgg ggaactgcgc
     2821 gcgcatgtcg tcgatctgga agccggcgcc ggtgtcgtgg atgccgttga tcaggatgag
     2881 cggccgcggc gcgatcagcg cgaggacgtc accgatctcg ccaatcgcct tgatccccgg
     2941 caccagctcg cagacgcagt gggggccgtt gaggtagccc tccaggctgc cgatgcagca
     3001 gacgggcgcc gcggccgcga cgcgctcgtc gagcgccgcg agatagagcg tctggttgca
     3061 ccctcccgag gcgccggtgc aggcgatgcg cgagggatcc acctcgggcc gtgaggccag
     3121 gtagtcgagc gcgcgcatgt tatcccacac ctggagaccg gggagcgtga gccccgtggg
     3181 aagcgcctgc gcgcccagct gccgcccgtg atactccgag ccgggatagg cgcgctcgcc
     3241 cgagccgatg gcatcgacac aaaggacgac gaacccgagc ttggccatgc cgatgttgcg
     3301 ctcctgcacc ctgggcgcca gtttcccctg ggaccaatgc ccgtgaaccg agaggatcgc
     3361 gggtgccggc tttggcgcct ccttcggaac gtagaggtgc gtggtcatca ccagattagg
     3421 gcgcgagtgg aaggtgaccc gctccagggt gtagtcgccc cggtcctcta cggccacgac
     3481 ctccgggcgc aggggacacg gctccgccag gctcaggccc atcgcctcgc gcagccgggc
     3541 acgccgggcg tctcgaatcg ccggccactc ctccggggtg gctggcagcg ggttctccgg
     3601 cgagatccgc gccgaccacg cgtggcagaa ccggtggaac atctcaccgg cgccctctcc
     3661 tatcaggtcc cactcggtgg cctcccctac tctcttgcgt tgcaccattg atcccttctc
     3721 ccaatcccga tccatcgcgc cggccggcga gcggtcaggc gctatcgcgg ccggctcatt
     3781 ccatgccgcg gaccgcctcg gtgaaaacct cctcggcggt gagggcacgc ccaaacacct
     3841 tcacgtcgtc cagcaggcca tcgaaggcat aggcgttctt gcgcccgatg tagttgccaa
     3901 tgattaggcc ccgtttggat ggcatgatct cgccttctgc gggcgccgcc gccttctcca
     3961 cgccgttgat gtagaggcgc atccatgtgc cgtcatggac tacggcgatg tggctccaga
     4021 agcccgcggg taccaaccac gacgccgact caacccgggg ctccttgcca tcggcggtgc
     4081 catactgaaa ctgggcgcgc gtccagtgga agcgaagccg ccacccgggc cacgggggct
     4141 ggccagcctt atcccccttg gaacagagga tttcgtaggt cttgttgcgc gccgagggct
     4201 tgatccacgc catggcggtg aggcccttcg ggggcatcag atcggcaaag ttcgccacgc
     4261 tcaggaacgc ctgttcaccc tcgcggagac ggagcgcctt gccgatgatc ccgggctcca
     4321 gcaccggctt ggcggtgccg gcagcttgaa aagtggcgtc gtgcccccgg cccgtggcgt
     4381 ctcgcacgat gccgtcgctc acctcgtcca tggtccagtg ggcgaggatg ccgcggtcgt
     4441 ccgcgcgagc ggcgggcgcc aggagaagaa gcgtcatgat gtggagcaga tagcgttgca
     4501 tcatcttccc tttcggcgtc acctggggtg cgaggcgggg gagcggccgc ggagtgccgg
     4561 tccgccggtg tcgctcggtc gcccctcccc cacattcagg cgctcacttc tcgtaggcct
     4621 cgatctccca cacccggctg agcgtctctc cctggcgcag gcgagtgatc tgcagccgga
     4681 tcactttcgt ctccacggga gcgaaggtcg cctcgatggc atccgtggta gcgttccgga
     4741 cggtggcgac ggtgcgccag ccctcgccat ccggcacctg gacctcgaaa tcggcgagcg
     4801 tgggcgagta ggtcaccacg cgcccgatcc tctgcgccgc cggccaggtg agggcaatcc
     4861 agtccgggaa ctgattcgga gtgccatcct gccacagcat gttggtcgtg acgccgtcca
     4921 cgacgcgctc cggcgtgcaa ccgtacttcg attccgagga gtagctgacg gtcacaccgg
     4981 agtcctcgaa ggcgagattc cccggcttct tgcgcgcggc attcgcggca tcgattgcac
     5041 gctggaggtc ggccaggcgc tcctgcccgg cgagttccgc agaggtggcg tagatatgaa
     5101 cggcataggg cgtgaacgtc tcgcgcagtc ggccatcacg cgcggcgacc gtgcgcccct
     5161 cggaaactac gtgaagctcc ctagccatct cctggggcag cgtcagctcc acggattggg
     5221 tttccgtggc ggtgttgacg acaaagagga cgctctgccc tcccgcgcgc cgcagcgaga
     5281 cgtggatgtg ctccggcttg ggcgcgttcg cgcggaccgc atcgggcacg tccggcgaga
     5341 ggacaaacgc cttcaggcgc tgcacctcgc gagccaggaa gggcatgccg aggccgatat
     5401 ccgggtagtt gccggcgtgg ctgaaggtgt accagaggaa gccagtggcg ccatacacca
     5461 ccgcctggta ggtcatgttg cgtagctccg tcaggttggg gccgcgctgg ccggcgcgcc
     5521 cgtagtcacc atagttgaag ccctgggggg tgatccagat cgccttgcgc ccgccggatg
     5581 cctcccgggc ggccttcatg aaggcgctca ccttctcgat cggttgcgcg gcgtatccgc
     5641 ccttgaggaa gcagggatag gggtcgggca tgagaatgtc gccgccgtcc gcgtacttat
     5701 ggatcccggc gatggtgtcg ttcagcatga tgcaggggtg gtagggatcc gcatcggcga
     5761 ccacctgata gagccggcgt gtgcgctccg ggagcgccgg acgcagctcc ggctcgtccg
     5821 ccatgtacca ggccaggatg ccgggatggt ccttgagggc atcaatgcgc gccgcgagtg
     5881 ccttggcgtc gtcatcgctc aagggctcgc cccaggcgtt catcatcttg gacgagggat
     5941 agggatagag ggtgacgtag gttttcgccg cggccacctc atccaggaac gcgcgcgcct
     6001 gctccaccga ctggtattgg gtgttgtagg tctgcatcgc cgtatagggg cagtcggact
     6061 tggccatctc tgccggggga atggagaacc acccaaaggg gagatacggc tcgccgttat
     6121 ggcgcagcac catctgctcg tcgaggcgcc actcgtgccc gttgggcgcc ggcggaagct
     6181 tgcgaatcgg cgctgtggcg acgcagatct gctttcctga gcgctcctgc acggccacct
     6241 ggagcaagta gctcccctct gccagcgcgc caataggaag gcgcagccgc gtctgtgcct
     6301 ccagcttgtc gactctcgtt gtggcgaccg gcttgccgcc cgcctggccc tcgggaacga
     6361 gcgagacgag ggcgcgtgcg ccggccatct cctcgggcga gagcgctagg cggagggtgg
     6421 cctcaacggc gtcgatcttc tcggtagcgt agatcgagtt gcggtaggcc ggccgggtga
     6481 cgatgatctc cgccgggacg taatcgagcg tgaccgggag ggagcggacg cagtagacac
     6541 ggtttggatc gcgccggtcg cgcaccgtta gaaccagcgt ctggttcccc tctcccggga
     6601 gcggggcctt gaagctgtac tcgcgaccct gcccggcgtc cagcatgtcc tgcacgggcg
     6661 ggccagccag gctcttccct gcctgcacga gcgtcggcac gagctcgata ggaagcaggc
     6721 gtccggtttc attcatcagg tgtagcttcg cccggtagag caaccggccc tcctcgcggg
     6781 ccacggaggc ttcgaagggc cgctccatct tccagaaata gcggctgagg gtagcgcccg
     6841 gcagctcgag ggaggcgtag agcgccggct ggtggaagcc gccggtggtc ggtgcgaagc
     6901 tggagagttg agtctggcct gcctggcgct cgcgtgtgac gttgagtgcc caggcaccgg
     6961 tgctcttccc ggtgaggccc agctccacaa acggaatggc cgcttctacc tgccacgact
     7021 gctcgccgat atgcgcctgc gccacccagt cgcagttcca ctccttgctg cgcacatgcc
     7081 cgccctggcg aagttcggcg tcgtagcgcg tgcccagagg gttgatggtg aagtgataat
     7141 actcgacccg ctcccccgtg ggatcgagca tgatctcgac gcagtcatcg cgatggacgg
     7201 ggccatcgtg ttcgcggacg gtggcatgca gatccttgat cgccggctcg gcgcattcga
     7261 tgccgaagta gaggcgcgag tcgtcgaaga gcaccttgaa gcgcgtctgc gggttcgcct
     7321 ggaccgtcac ctgatcgagt agggtgaagc cgccatacca ggtggcggcc tgccaagcgg
     7381 cgtcgtcgag ccggccgtcg atcttcgggg cgacggcggc gcgtgtcgcg acgactttcg
     7441 gcggtttcgg ggcttcaccg gcccagatcg ccacgggtac catggcgaga agcagataga
     7501 ggaaccgctt gatcattgct actctcactc cctatgcttc ctgctcgccg cgcggcgatg
     7561 ctcagagccc gagcaaccgg atcgcgttct ctcgcgcgat cttgcggaac acctcctcgc
     7621 tgagcttgcc ggatgccttc aggccgagca ggaactctac ctggggcgcg ggctggtccg
     7681 cccggcagat atcggtgccg aagaggagcc gatcctggaa ctcgttcagg aagcggaccg
     7741 cgtagtcggg atcgcgcgcc agggcgttgt agccgctgcc ggcggagagg tctccataca
     7801 gattcgggta gcggcggaac agcttcggga cgacgccctc ctcgtggatc ggatagcgcg
     7861 ggtaggaacc ccggtcggca ggggtctcca ggcggccgat ctcggcccag aacgccggtc
     7921 cgtgtccgag gataatcagg cgcgggaagc gctgcagcgt agtctccagc tgcggcagac
     7981 cctgatcatc atagaggccg taagtaccgc cgatgcgcgt ggagatatcg aagatgacgg
     8041 ggaaacccac gtcttgggcg tgcttgaaga gattttgaac gcgcgggtcg aggaagggaa
     8101 ggttgggcat cagctcgcca atccccttgc acccctggtc gcggtagtag cgcaggacat
     8161 cgcccagggg cgcgtctggt gagttggaga gggcgcgcgg gtccacgttg cagaagggaa
     8221 tgaagcgatc cgggtagcgc tcggcgatct ccaggatctc ctcgttcgac tccggcaggt
     8281 agacctctgg cccgatcagt ggaagtaggc aggccttctc aatcttcagc tcttcatacc
     8341 gctccaggag ctgctccggg gtgacgaagt ggatccgtcc gtcctgcttc ggcccgatct
     8401 tcttgtaggc atgcgcgtga atgtcgatga acatggtccc cctcctcccc ggtctggttc
     8461 gcacgcccga ggaacaactc ctgccctgga tgcactgcga aaccgcgccc ttggggaatc
     8521 gagcggattg ccggcagact cacctccctt cccacgggcc tgtcagacgg ctgtcagata
     8581 ccctctcgcc
//
LOCUS       DAOSCV010000044         8530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5416_length_8530_cov_3.121180, whole genome shotgun sequence.
ACCESSION   DAOSCV010000044 DAOSCV010000000
VERSION     DAOSCV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8530
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_8530_cov_3.121180"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /locus_tag="PLY56_02005"
     CDS             <1..1592
                     /locus_tag="PLY56_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20283.1"
                     /translation="KTATETLDTIEKRGYINAREVDQIEASLIMAAHAAPNLRELGRS
                     KEQGLPYVSYVPRAMSNTRILPQEPIVPARMAREIEMAACPGEYESATFILQAVAPVR
                     SLTLEATDIQGPRGRIPKDAVDLYVVKCWYQAGTEIGFTQQRLLAPELLLKDDALVRV
                     DHEKKENFIRSTDAAGKSMYLPCSGPTSDHLANVRPKDADTLQPVDIEMGTARQFWIT
                     LKVPEGTPAGEYEGAIRIRAQGAPASEMRLKLRVYPFNLEPCPLQHAIYYRGVLRPDG
                     QGSISSEAKSEEQYEAEMRDLLAHGVLYPTIYERYDETLLRRALEIRKKAGLPTGPLY
                     VLGLGTGSATDPKALAAHQAKLRQYMDVAREFGYTDVYFYGTDEAKGERLTIQRAAWK
                     SVQDAGAKTFVACYLGTFEAMRGLLNLAVLAGRPNPEEATKYHGVASRIFCYAYPQVG
                     NEEPETYRRNFGLVLWKAGYDGAMDYAYQHSFGHVWNDFDHATYRDHCFTYPTVNGVV
                     GTIQWEGYREAVDDVRYIATL"
     gene            1882..2952
                     /locus_tag="PLY56_02010"
     CDS             1882..2952
                     /locus_tag="PLY56_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20284.1"
                     /translation="MRKWRVGLVGIGRGSGYGRLFAEEPRCEIVACCDASEEALARFQ
                     RELELPDSQCFTRYDDFLAAGPEIVFLGTPIPVHADQTVAAMEAGCHVLSEVTAASTL
                     ADCQRIIDAVHRTGRTYMMAENCIYWHFVSQWKEWVQSGRLGTIVHAECEYLHPIPSL
                     IFDRETGKARWRTQRAPLHYCSHSLGPILEITGDRITRAFGLGNSRHVLPEGGIGAID
                     MQVALFETASGMTIKLLRTSVLPRRPNIHFYSLHGTKGYVETDRGGLKGPGRLYVEGE
                     MESDQAIETPLSDPSLPESAHAGGHGTAEYILIQDFLAALERGTKPRIDEIRAMEMTA
                     PGIVAHESAMRGGAWLEVPSFA"
     gene            complement(3050..3481)
                     /gene="fucU"
                     /locus_tag="PLY56_02015"
     CDS             complement(3050..3481)
                     /gene="fucU"
                     /locus_tag="PLY56_02015"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOJ20285.1"
                     /translation="MLKGISPLLSPELLDALYRMGHGDEIVLADAHFPGETVGKRVVR
                     ADGLRIADLLDAILPLFELDSYVDAPVVMMAAVPGDTLDPAVEAAYRAAIDRHAPGTP
                     AIARIDRFAFYERAKAAYAVVMTGETAKYGNILLKKGVTPC"
     gene            3770..3955
                     /locus_tag="PLY56_02020"
     CDS             3770..3955
                     /locus_tag="PLY56_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HOJ20286.1"
                     /translation="MEKKEKLHEKMVEAGKKLGEARKRELGHEGYEELGRKGGEAGGA
                     RVRHLVREGKKAERQGE"
     gene            complement(4094..5452)
                     /locus_tag="PLY56_02025"
     CDS             complement(4094..5452)
                     /locus_tag="PLY56_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20287.1"
                     /translation="MYQIRPRRLFAMDWAMADERNIRRMERMVRGMGRDPSEVRVLAA
                     EELPDVIRESGWIGEVRQGAYDMVRDPDFIFTAFRWVSDAERTEIMRSDLFRRCVEAH
                     RTYGDCKQWFTGGRILAMLGAAPIHHYEKRPEWDPKLVCWSLYDIHSAWGCVHRCAYC
                     QRGSVYVINLNLEEFVERVDQLLEQNPWQKTIRYDVEQDVLAIEPEYGACELLVNHFA
                     RLDDRYLILFSKSANVDHLLSLDHRGHTIILWTLCSRTVSRRYEARTGSMEQRIEAAA
                     KCSEAGYPVRFKCKPVIPIRGWREETTEMLERLYARVKPESISMEMVFFDSVAEMDAT
                     LGLENLDPEFVEAAREAEARYGDQWRHDLEGPRPFTFEVKEKVYRHLITESKRLSPET
                     PVTLCAETQRMWDALAPLIGYREYDYVCNCGPLCTPGLRRIESVSGPDAERIAERAAA
                     AA"
     gene            complement(5518..7017)
                     /locus_tag="PLY56_02030"
     CDS             complement(5518..7017)
                     /locus_tag="PLY56_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ20288.1"
                     /translation="MSQTQQVVLIMTDTQRFDMLGCYGNPDMRTPCLDRLAAGGVRFD
                     RAYTCQPVCGPARSALFTGTFPHSNGMWGNSMALGDNVKTIGQRLRDQGVHTAYIGKW
                     HLDGGDYFGVGRCPDGWDPEYWYDMRCYLEELTPEERVRSRQTESCEDPELTAEFTFG
                     HRCSNRAVDFLRRFHESPFFLVVSYDEPHEPYLCPKEYVDLYRDYEFPKSRNIWDTLD
                     GKPEHQRVWAGARLKADKDALRIRPAHYLGCNTFIDQEIGRVLDAIDEYAPGALVIYT
                     SDHGVFLDSHSLAGKGPAMYDEITRVPLLVRWPGVIPEGTTCPHPASHIDLVPTILDA
                     FGFAIPRILEGKSMMPALRDPGQRVNDVIFCEFGRYEIDHDGFGGFQPVRCAFDGRHK
                     LVINLMTSDELYDLERDPEEMENRINAHEYAALRDALHDRLLDWMNRTRDPFRGYYWE
                     RRPWRTDAREATWAYTGYTRQRENEEYEPRQLDYDTGLEMVEAHRIKKL"
     gene            7286..>8530
                     /locus_tag="PLY56_02035"
     CDS             7286..>8530
                     /locus_tag="PLY56_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ20289.1"
                     /translation="MTPINHAGARWLTKIRLPLAAAAPLLSAILWGVLFIAVHPSRQE
                     FPLHDDWLYARWLFDLARGEGINYRGLTVMPLLGQWLWALPFVRTFGDSLVTLRLSTI
                     LLSWIGMVALMDLLRQAGVPRGRAVLATACLAANPYFFLLSGTFMSDVPALSFSLVAL
                     ALSGRAIRGERFAWMAAAALAATLATITRQNALVVPLVMAAFLWQHPRLRARPAWNAA
                     VLLPAALGGVTALWFASRPDTLRLVPAVPNWDRAAILLFAGIHTLALASAPLLLLAPA
                     AASARRFYLALGLFLAVAFAYVLDAQAFPYIGNTIPCFVGMGDVPGSRPVLINEDVQT
                     LITVLGCIAAALLIATSAGYIRSNWWRQPLLLFTMLHALVLLFSPFLYDRYLIPLLPG
                     AFLAAAAGSGAERARPWPALITL"
BASE COUNT         1422 a         2835 c         2773 g         1500 t
ORIGIN      
        1 tgaaaaccgc caccgagacg ctggacacca tcgaaaagcg cggctacatc aacgcgcgcg
       61 aggtagacca gatcgaggcg agcctgatca tggcggcgca cgcggcgccg aacctgcgcg
      121 agctcggtcg gagcaaagaa caggggcttc cctacgtctc ctatgtgccg cgggcgatga
      181 gcaacacgcg catcttgccc caggagccaa tcgtgccggc gcggatggcg cgcgagatcg
      241 agatggctgc ctgccccggc gagtacgagt ccgccacctt catcctccag gctgtggcgc
      301 ctgtccgcag cctcacgctt gaggcgaccg acatccaggg gccgcgcggg cggatcccca
      361 aggacgcggt cgatctctac gtcgtgaagt gctggtatca ggcggggact gagattggct
      421 tcacccagca acgcctcctg gccccggagc tcctcctgaa ggatgacgcg ctggtgcgcg
      481 tcgatcacga gaagaaagaa aacttcatcc gctccaccga cgccgccgga aagagcatgt
      541 acctcccgtg cagcggaccc acgagcgacc acctggcaaa cgtgcgtccc aaggacgctg
      601 atactctcca gccggttgat atcgagatgg gcacggcccg gcagttctgg atcacgctga
      661 aggtgcccga gggcactccc gcgggggagt acgagggcgc gattcgcatc cgcgcgcagg
      721 gagcccccgc ttctgagatg cgcctgaagc tgcgcgtcta tcccttcaac ctggagccct
      781 gcccgctgca acacgcgatc tactatcggg gcgtcctccg gcccgacggc cagggctcga
      841 ttagctcaga ggcgaagtcc gaggagcagt atgaggcgga gatgcgcgac ctcctcgcgc
      901 acggtgtcct ctacccgacg atctacgagc ggtatgatga gacgctcctg cgccgggcgt
      961 tagaaatccg caagaaagcg gggcttccta ccggcccgct ctacgtgctc gggctcggca
     1021 cgggcagcgc cacggacccg aaggctctgg cggcgcatca ggcgaagctg cgccagtaca
     1081 tggacgtcgc tcgcgagttc ggttacaccg atgtttactt ctatggcacc gacgaggcaa
     1141 aaggcgagcg gctgaccatc cagcgcgctg cctggaagag cgtccaggat gccggcgcga
     1201 agacctttgt cgcgtgctat ctgggcacct tcgaggcgat gagagggctc ctgaacctgg
     1261 cggtgctcgc cgggcgcccg aatccagagg aggccacgaa gtaccatggc gtcgcgagcc
     1321 ggatcttctg ctacgcctat ccccaggtgg ggaatgagga gccggagacg taccggcgca
     1381 acttcggcct cgtcctctgg aaggcgggct acgacggcgc catggactac gcctaccagc
     1441 actcgttcgg gcacgtctgg aacgacttcg accacgccac ctaccgcgac cactgcttca
     1501 cctaccccac ggtgaacggt gtcgtgggta ccatccagtg ggagggctac cgtgaggcgg
     1561 tggatgacgt gcgctacatc gcgacgctgt aaaaggcgat cgagcgcgcg cgtgcccggg
     1621 aggcgaccgc cgcgctggcc gcggaggccg cccggtggct ggaaggggtc gacccgcaag
     1681 gcgacctgga cgcgatccgc cagcaggccg cggagtggat cacgcggctc tcggctcagc
     1741 ggtgataggt gagcggtgcg agccaggcag gggccgacgt ccgggggggc gaactctcac
     1801 tccgctggcc tgaaggatcg cggaggcctc gcctccccag aactcggcca ggcatactgg
     1861 gcacgtgtga ggagcaacag gatgagaaag tggcgagtcg ggttggtggg catcggtcgg
     1921 ggctccgggt atggacgcct cttcgcggag gaaccgcggt gcgagatcgt ggcgtgttgc
     1981 gacgcctccg aggaggcgct ggcgcgtttc cagcgcgagc tggagctacc ggacagccag
     2041 tgcttcacgc gctacgatga cttcctcgct gccggcccgg agatcgtctt ccttggcacc
     2101 cccatcccgg tgcacgccga tcagacggtg gcggcgatgg aggcgggctg ccacgtgctg
     2161 agcgaggtca ctgccgcatc caccctggcc gattgccagc gcattatcga tgcggtccat
     2221 cggaccgggc gcacctacat gatggcggag aactgcatct actggcactt tgtctcccag
     2281 tggaaggagt gggtgcagtc cgggcggctg ggcaccatcg tgcacgccga atgcgagtac
     2341 ctccatccga tcccctcgct catcttcgac cgggagacgg gcaaggcgcg gtggcggacg
     2401 cagcgcgccc cgctccacta ctgctcgcac tcgctcggcc cgatcctgga gatcaccggc
     2461 gaccggatca cccgcgcctt cggcctgggg aacagccggc acgttctccc cgagggcggc
     2521 atcggcgcga tcgatatgca ggtcgcgctt ttcgagacag cgagcggcat gacgatcaag
     2581 ctcctccgca cctccgtcct gccccgccgg cccaacatcc acttctactc gctccacggc
     2641 acgaaaggct acgtcgagac ggaccgcggc gggctgaagg gccccggccg gctctacgtc
     2701 gaaggcgaga tggagagcga ccaggcgatc gagacgcccc tttccgatcc gtcgcttccc
     2761 gaatcggcgc acgccggcgg ccacggaacc gccgaataca tcctgatcca ggacttcctg
     2821 gcggcgctgg agcgcgggac gaagccgcgc atcgatgaga tccgggcgat ggagatgacg
     2881 gcgcccggga tcgtggccca cgagtcggcc atgcgcggcg gtgcctggct ggaggtgccc
     2941 agcttcgcct gagcggcccg gctggcagtg acgcagagat aaagagcgcc ctcctgctcg
     3001 cggcgagagg cccaggcggg agggcgctgg tgatacgaaa cccggggctt tagcagggcg
     3061 tgacgccttt cttcaggaga atattgccgt acttggctgt ttcaccggtc atcaccaccg
     3121 cgtaggccgc tttcgcgcgc tcgtagaagg cgaagcggtc gatccgggcg attgccggcg
     3181 tgccgggcgc atgccggtca atggcggcgc gatacgccgc ctcgacggcg ggatccaggg
     3241 tatcgcccgg caccgcggcc atcatcacca cgggcgcgtc cacgtagcta tccagttcaa
     3301 agaggggcag gatcgcgtcg agcaggtcgg caatccgcag gccatcggca cgaacaacgc
     3361 gcttgccgac tgtctcgccc ggaaaatgcg cgtcggccag gacaatctca tctccatgcc
     3421 ccatgcgata gagggcatcc aacagctcgg gactcaatag cggcgagatc cctttcagca
     3481 tctctccgaa ctcctctcag ggtctgcgcg gctccctggc tccaagccag cggcgcgatg
     3541 agggatctgg gagcgcgcct tcttgcctgg agcgggcgta tgccgctgcg ggcaaccgaa
     3601 tctcggttcc gcgccgcact ccccttcccc tgcagaggca cgtgtcacaa ccgggcgttc
     3661 cacgcgggcc cgtggtttcg ttcgccagga tgtcgcgccc gggttggaag agcctcgttc
     3721 gtggtttggg ccgggttggc accggctatg agctatgagg gggatgacga tggagaagaa
     3781 ggagaaactt cacgagaaga tggtggaggc cgggaagaag ctcggcgaag cccgcaagcg
     3841 ggagttaggc cacgagggct acgaagagct gggccgcaaa ggtggcgagg cgggaggggc
     3901 tcgcgtgcgc cacctggttc gcgagggcaa gaaggcagag cgccagggcg aatagccagg
     3961 aacgaaggaa gcaagagatt cgcatcgagc tgcgcgaagg cggagcgtgg ctgccaccgg
     4021 gagaagcccg gccgggcttc tcccggtgtt tcgtaagagg cgtgaaggca cgcggcgcgc
     4081 catccaggag tgatcacgca gcggccgcgg cccgctccgc gatccgctct gcatccgggc
     4141 cggaaacgct ctcgatccgc ctcaggcccg gcgtgcagag ggggccgcag ttgcacacgt
     4201 aatcatactc ccgatagccg atcagtggcg ccagggcgtc ccacatccgc tgcgtctctg
     4261 cgcagagcgt caccggcgtc tccgggctca accgcttcga ctcggtgatc aggtggcggt
     4321 agaccttttc tttaacctcg aaggtgaagg gccgtggccc ttccagatcg tggcgccact
     4381 ggtcgccgta gcgcgcctcc gcctcgcgcg ccgcctcgac aaactcggga tccaggttct
     4441 ccaggccgag cgtggcatcc atctcagcga cgctgtcgaa gaacaccatc tccatgctga
     4501 tgctctccgg cttcacgcgc gcgtagaggc gctccagcat ctcggtcgtc tcctcgcgcc
     4561 agccccggat cgggatcacc ggcttgcatt tgaaccggac gggatagccg gcctcgctgc
     4621 atttcgcggc ggcctcgatc cgctgctcca tcgagccggt ccgcgcctcg tagcgccggc
     4681 tgaccgtgcg cgagcagagc gtccagagta tgatcgtgtg cccgcgatga tccagcgaaa
     4741 ggaggtggtc cacgttggcg ctcttggaga agaggatgag gtagcggtcg tccaggcggg
     4801 cgaagtggtt caccaggagc tcgcatgcgc cgtactccgg ctcgatagcg agcacgtcct
     4861 gctccacgtc gtagcggatc gtcttctgcc aggggttttg ctccaggagc tggtcgacac
     4921 gctcgacaaa ctcctccagg ttgaggttga tcacgtagac cgagccgcgc tggcagtagg
     4981 cgcaccggtg aacgcacccc caggcgctat ggatgtcgta caggctccag cagaccagtt
     5041 tgggatccca ctcggggcgc ttctcgtagt ggtggatcgg cgcggcgccc agcatcgcca
     5101 ggatccgccc accggtgaac cactgcttgc agtcgccata tgtccggtgg gcttccacgc
     5161 accggcggaa gagatcgcta cgcatgatct ccgtgcgttc ggcatcactc acccagcgaa
     5221 aggcggtgaa gatgaagtcg gggtcgcgca ccatgtcgta agcgccctgg cgcacttccc
     5281 cgatccagcc gctctcgcgg atcacgtcgg gaagctcctc agctgcgagc accctcacct
     5341 cggagggatc gcggcccata ccccggacca tgcgttccat ccggcggatg ttccgctcat
     5401 ccgccatcgc ccaatccatc gcgaagagcc tgcgtggcct gatctggtac atcccgaatc
     5461 ctcctcacgg cgggcgcggt ctcggcaccg ctggaatcca cccgcgctcc cggcgcgcta
     5521 cagcttcttt atccggtgag cctcgaccat ctccagcccg gtatcgtagt ccagttggcg
     5581 gggctcatac tcctcgttct cccgctggcg cgtgtagccg gtataggccc aggtggcctc
     5641 gcgcgcgtcg gtgcgccagg gccggcgctc ccaatagtaa ccgcggaagg gatcccgagt
     5701 ccggttcatc cagtcgagga gacgatcgtg cagcgcgtcg cggagcgccg catattcgtg
     5761 ggcgttgatg cggttttcca tctcctccgg gtccctttcc aggtcataca gctcatccga
     5821 ggtcatgagg ttgatcacca gcttatgccg gccgtcgaag gcgcagcgca ccggttgaaa
     5881 gcccccgaaa ccatcgtggt cgatctcata ccggccgaac tcacagaaga tgacgtcgtt
     5941 gacccgttgc ccggggtcgc gcagcgccgg catcatgctc ttcccctcga ggatgcgcgg
     6001 gatcgcgaag ccgaaagcgt ccaggatggt aggcacgaga tcgatatgcg acgccggatg
     6061 cgggcacgta gtgccctccg ggatgacgcc aggccagcgc acgagcagcg gcacgcgcgt
     6121 gatctcatcg tacatcgccg ggcctttgcc ggcgaggctg tgcgaatcga ggaaaacgcc
     6181 gtggtccgag gtgtagatca ccagggcgcc cggcgcgtac tcatcgatgg catccagcac
     6241 ccggccaatc tcctgatcga tgaaggtgtt gcagcccagg taatgcgccg ggcggatgcg
     6301 caatgcatcc ttatccgctt tcagcctcgc gccagcccac acgcgctgat gctccggctt
     6361 tccatccagg gtgtcccaga tgttccggct cttggggaac tcgtagtccc gatagagatc
     6421 gacgtactcc tttgggcaga ggtatggctc gtgcggctcg tcataggaga cgaccaggaa
     6481 gaagggcgac tcgtggaagc ggcgcaggaa atccacagcc cggttcgagc aacggtgccc
     6541 gaaagtgaac tcggccgtga gctcgggatc ctcgcagctt tccgtctgcc gcgagcgtac
     6601 ccgctcctcc ggggtcagct cctccagata gcagcgcatg tcgtaccagt actctggatc
     6661 ccagccatcg gggcagcggc cgacgccgaa gtagtcgcct ccatccagat gccacttccc
     6721 gatgtaggcg gtgtgcactc cctggtcgcg taggcgttgg ccaatcgtct tcacattgtc
     6781 gcccaaggcc atgctgttgc cccacatgcc gttggagtgc ggaaaggtgc ccgtgaagag
     6841 cgcggagcgc gccgggccgc acaccggttg gcaggtatag gcccggtcga agcggacgcc
     6901 cccggccgcc agccggtcga ggcagggcgt tctcatgtcg ggattgccgt agcatcccag
     6961 catatcgaaa cgctgggtgt ccgtcatgat cagtaccact tgctgtgtct ggctcatgga
     7021 agctcctcga aagccttggg ctcgcaccgg tattacacgt gcgattcccc ccgctggccc
     7081 cggtctcctc cctgccctgc cgcttcagtg accatccgta agagagagcg gttgcgcatg
     7141 acgggccggc ggggctgggc cgcaccgagg ccacacggcg cctgctcctg gcggcaggaa
     7201 tcgcggctcg ctccgggaac ttccggatag gcccacgccg tggaagggcg aacgcgatga
     7261 gcaacgagac agtgcagatc gacctatgac gcccatcaac cacgccggcg cccgctggct
     7321 taccaagatc cgcttgcccc tggcagccgc ggcccctctc ctctcggcga tcctctgggg
     7381 agtgctcttt atcgccgtgc atccctctcg ccaggagttc ccgcttcacg atgactggct
     7441 ctatgcccgt tggctcttcg atctggctcg gggtgagggc atcaactatc gcggtctgac
     7501 cgtgatgccg cttctgggcc aatggctgtg ggcgctccct tttgtgcgca catttggcga
     7561 ttcgctggtc accctccgcc tctccacgat tctcctctcc tggatcggga tggtggctct
     7621 gatggatctc ctgaggcagg cgggggtgcc cagggggcgg gcggtgcttg ccactgcttg
     7681 cctggcagcg aatccctact ttttcctgct gagcggcact ttcatgtccg atgtaccggc
     7741 gctctccttc tctctcgttg cccttgccct ctctggaagg gcgatacgcg gagaacggtt
     7801 tgcctggatg gccgccgccg ctcttgccgc gacactcgcc accatcaccc gccagaacgc
     7861 gctggtcgtg cccctagtga tggcggcctt cctctggcaa cacccgcggt tgcgtgcgcg
     7921 gccggcatgg aatgccgccg tgcttcttcc cgcggcattg ggaggagtga ccgccctttg
     7981 gttcgcgagc cgcccggata cgctgcgcct tgttcctgcg gtgccgaact gggatcgtgc
     8041 cgcgattctc cttttcgccg gcatccacac cctggctttg gcgtccgctc ctctgcttct
     8101 tctcgcgccc gcggctgcct ccgcccggcg cttctatctg gcgctcgggc tcttccttgc
     8161 ggttgcgttc gcctatgttc tggacgcgca ggcctttcct tatattggca acacgatccc
     8221 ttgcttcgtc gggatgggag acgtgcctgg ctcacggccc gtgctgatca acgaggatgt
     8281 tcagaccctt atcacggtgc tcggttgcat cgccgccgcg ctcttgatcg ccacgagcgc
     8341 cggctacatc cgcagcaatt ggtggcgcca gccgctactt ctcttcacca tgctccacgc
     8401 gctggtgctt ctcttctcgc cgtttctcta cgaccgctac ctcatcccgt tgcttccagg
     8461 ggcattcctg gccgcggcgg ccggatccgg ggccgagcgc gcgcggccgt ggccggcgct
     8521 catcacgctc
//
LOCUS       DAOSCV010000045         8473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5455_length_8473_cov_3.444642, whole genome shotgun sequence.
ACCESSION   DAOSCV010000045 DAOSCV010000000
VERSION     DAOSCV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5455_length_8473_cov_3.444642"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="PLY56_02040"
     CDS             <1..1326
                     /locus_tag="PLY56_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ20290.1"
                     /translation="PQPRVLTLQDSVAIALERNRSFQIAQDQLAIAQARVAEAIAPTR
                     IQALAQASITRNDKASTITLPGAEGGAQSVEISPLYSRNAQLVVSKQLDISGALRTSR
                     SIAELGVLAAQIELQRTREQTVLEVRNAFYQVLRAQAALEVAEANLASLEEHLRQAQA
                     FYREGVKAQFDVLRAETQVANARQSVIAARNAVDLAKAALNNTMGIDVTTPVVLQTEE
                     EVTPVVPQFSESVALAYENRPEVQQAMVAIEVAQRGVSLARTGNRPTLALSWNANATA
                     DGGAFQSRAFQWNAVAAVSMPIFEGGLTRARVRQAQGEVETAIVTEDQVRQAVALDVQ
                     QAILSMSDAVERMQSAAKNVDQAREALRLAKLRYQEGISTTLEVTDAQAALTQAENNL
                     VNARYDYLQARARYLRAIGSTFTQAEALPVTGAGQQGTTPRESQAAVKP"
     gene            1355..2299
                     /locus_tag="PLY56_02045"
     CDS             1355..2299
                     /locus_tag="PLY56_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20291.1"
                     /translation="MIADPRKPERSPTDIVLRANGLCKRYGKRWVVDGVSLEVRRGDV
                     FGFLGPNGAGKSTTMRMLTGLVRPDAGTVELLGHDLHRRPTAALARVGAIVEAPALYP
                     HLSADRNLEVFSLLSGGASPERRAEVLRLVGLEGRGHDRVKSYSHGMKQRLAIAQALL
                     PHPELILLDEPTNGLDPGGMVEVRQLLRHLASQEGITLFLSSHLLSEVEQVCNRVAVI
                     DQGHVLVQGEVRELLREQTFLQITAEPVELARQILETLDYVRQVIVQEGTLRIDAPLD
                     SAAAINAALVHGGCRVSALVPGRQSLEEFYLGLLEKRS"
     gene            2296..3123
                     /locus_tag="PLY56_02050"
     CDS             2296..3123
                     /locus_tag="PLY56_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ20292.1"
                     /translation="MTHLLRVEILKLSRRPRTYLGYAGILLLLVPMLLGLLYGQPDRH
                     LRHEIGPEFHVVGTFVNALFMARRMMEPCLWFFLPLFIAMVAGDLVAGEAAEGTLRAL
                     LVRPVRRVPVLLAKFTVAVLHATALTYFLGLSALGLGTLFFGWGDLILIEHGIYVLGS
                     GEALARLAAAYTAAIPGMVVVAALALFLSVLVDNSNGAIIATMMLLFVSFTLGGLPYF
                     EAIQPYLFTTHMGVWSSLFEDPIPWSEIARSMAWLAAYCIAFVGAAALVFSRKDILS"
     assembly_gap    3217..3226
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3401..5116
                     /locus_tag="PLY56_02055"
     CDS             3401..5116
                     /locus_tag="PLY56_02055"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20293.1"
                     /translation="MATAKRLPLETKATAKTPVIGVFATGDPRIDQASRDRAVNIVEM
                     AANVIAGGVKLPDGTSVPVVYSPVLVDGEREADIVARQFQAEGVDILVCVPDTWAFPQ
                     LGLISLVSQFPEETPLNLTCGNSGPRPGVVLTHAANGAFSQYGRLVHINVGTWPDTGQ
                     NPQMTPATAKSLIDWVYAAVTRQAIRGRRVAIFGHDSMGMETALAHVIPTRQTFGLEI
                     TRLDMKLLADMLQKKAYDAKELEALRAWLFEHVGPRIEIRDDADRERLDQGLAMYLIT
                     RDLLKDLNAVGGGFMNQLEWGSDRRGIPLPVADVMESLFNSSFDHNGPKPPLPFATEA
                     DTQALLTMLAFTYLGGGTPPLFMDFRKVWEPWEINQLADSLGMGDVEPDALWRRKGLV
                     DGNNSGSASFDWAGKPGDSVKELLLRVSMPQADPDYFPGGGNSVTFITPGGIEGIAGR
                     LGYNTLSGLFSLIWDEAVTVDLPEKLAHAVANTSNPTWPHTWVVPKYATMGEYKQFAP
                     ANHFHMNWGLNPARLEYWMDLANVLSATPWQDRPSYIEGVDRPTPLLYIQNGGEATAK
                     MRRKR"
     gene            complement(5166..5909)
                     /locus_tag="PLY56_02060"
     CDS             complement(5166..5909)
                     /locus_tag="PLY56_02060"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOJ20294.1"
                     /translation="MDEIRERYQRVLEEYLTLDTLLRNVENTRFRVCIFGSARIRPTD
                     ETYRLVLNLARQLAYEGIDIVTGGGPGLMEAANRGVHEARTGVSTSYGVTIELPTLAE
                     MANKHLDVKSSHKRFSLRLDEFMRLTHAVIVASGGIGTLLELAYVWQLLQVGLIEERP
                     VVLLGKRLWGGLIDWMNREMVRCGFVSPEDMELVQVVDSPEEALEIVREAHQRFLDAL
                     AQRSEAGAPGEMPPAGEAHPPLPSGAPPA"
     gene            complement(5902..7095)
                     /locus_tag="PLY56_02065"
     CDS             complement(5902..7095)
                     /locus_tag="PLY56_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ20295.1"
                     /translation="MKPGALRVRLQIHYLLLFAPFAIATTYQNLHFKRCGFTESEIGT
                     LNALAALLAVLSPPLWGMVTDRAGDKRIPLALLFLASGATYPLFLLADSLGSALLIQA
                     VFNFSFAPCIPLTDAIALELLPRAGGDYGRIRLWGSLGFIVTLLVSGLFLGGGPLAPK
                     GGGDLVATFVAFGIVRVLGAAWVLSLPRGEAPQAVATYRGDLGALFRDRGFLLFLAAT
                     FLCNTALRSYYVFLSIYLDGLGVSDSMKGVFWSLGVAAEVVFMVFAWRLVQRMGVCGM
                     LMLGMAGGALRLILFSLFLPVGAVALAQGLHALAFGATHIASLTFINASVPERFRASG
                     QTLHAAIVGGLGGVLGSRLAGDLAQAWGIPAAFRACGLLAGVAALLVVLLPERQTGTG
                     EEKSG"
     gene            complement(7206..>8473)
                     /locus_tag="PLY56_02070"
     CDS             complement(7206..>8473)
                     /locus_tag="PLY56_02070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ20296.1"
                     /translation="EKLAIEGGTPVTKDPFPAWPWFDEETIQAAMEPLRNGKVNYWTG
                     PLGMQFEQKFAEWCGARFGISTSNGTSALHTALAGLGIGPGDEVIVPSYTFIASSFSV
                     CQAGAIPVFADVEKESHTLDPASVEAAISDRTRAILTVHLYGIMSNMGPILEIAKKHN
                     LFVIEDCAQAHGAEYKGKRAGTIGDIGAFSFCQSKHFTTGGEGGCVVTDDEDVAWLCR
                     SFRDHGYDVAQRLKLLELEAKLPYIHNMVGFNYRMTEMQSAIGLVQLSKMDNWNLKNR
                     RRNGEMLTRLLQSCEEIETLPLDTPERRNSYWLYPISMRMDRLTCDIKQFTAALGAEG
                     IPAGPVLWPQCYQEKAYQERRGFGRLNYPFGDPSARPEAVQYEKVFCPNAAYLEKNTF
                     FVPVHPVYEEKHMEQIAAAILKVAKAYAK"
BASE COUNT         1499 a         2918 c         2638 g         1408 t
ORIGIN      
        1 ccacagcccc gcgtcctcac gctgcaggat agtgtggcga tcgccctgga gcgcaaccgc
       61 tcgttccaga tcgcccagga ccagctcgcc atcgcccagg cacgcgtcgc cgaggcaatc
      121 gctcccacgc gcatccaggc cctggcccag gcgtcgatca cccgcaacga caaggccagc
      181 acgatcaccc tccctggggc tgagggcggg gcgcaatcgg tcgagatctc cccgctctac
      241 agccggaacg cgcagctggt ggtcagcaag cagctcgaca tatccggcgc gctccgcacg
      301 tcgcgtagca tcgccgagct gggcgttctc gccgcccaga tcgagctaca gcgcacgcgc
      361 gagcaaacgg ttctggaagt tcgcaacgcc ttctaccagg ttttgcgcgc gcaggccgcc
      421 ctggaggtcg ccgaggcaaa cctggcctcg ttggaggagc accttcgtca ggcacaggcg
      481 ttctaccgcg aaggtgtgaa ggcgcagttc gacgtcctcc gcgccgagac gcaggtcgcc
      541 aacgcgcgcc aaagcgtcat cgccgctcgc aacgccgtgg atctcgccaa ggcggcgctc
      601 aacaacacaa tggggatcga cgtcaccacg ccggtcgtcc tccagaccga ggaggaggtc
      661 acgcccgtcg tcccgcaatt ctccgagagt gtggcgctcg cgtatgagaa ccggcccgag
      721 gtgcagcagg ccatggtcgc catcgaggtg gcgcagcgcg gcgtctcgct cgcccgcacc
      781 ggcaaccggc ccacgctcgc cctcagctgg aacgccaacg caaccgccga cggcggcgcg
      841 ttccaatcga gggcgttcca gtggaacgct gtggccgccg tctcgatgcc gatcttcgag
      901 ggcgggctta cccgcgctcg cgtgcgccag gcccagggcg aggtagagac cgccatcgtc
      961 accgaagacc aggtccgcca ggccgtcgcc ctggatgtgc agcaagcgat cctctccatg
     1021 tcagacgcgg tagagcgcat gcagtccgcg gccaagaacg tcgatcaggc ccgcgaggca
     1081 ctgcgcctcg ccaagctccg ctaccaggag ggcatctcca ccacgctcga ggtgaccgac
     1141 gcccaggccg cgctcaccca ggccgagaac aacctggtga acgcgcgcta cgattacctc
     1201 caggcgagag cgcgctacct gcgcgccatc ggctcgactt tcacgcaagc cgaggcgctt
     1261 cccgtgactg gcgccggcca gcaaggcacc acgccgcgag agtcgcaagc cgcggtgaag
     1321 ccgtaagacc tcttgtgata ggaggagagc gggcgtgata gccgatccac gcaagcccga
     1381 gcgttcgccg acagatatcg tcttgcgcgc gaatgggctg tgcaagcgct atggcaagcg
     1441 ctgggtggtc gatggcgtca gcctcgaggt gcgccggggc gatgtcttcg ggttcctcgg
     1501 gcccaacggc gccgggaaga gcacgacgat gcgtatgctc accggcctcg tgcgccccga
     1561 tgccggcacg gtcgagttgc tcgggcatga cctgcatcgc cggcctaccg ccgcgctcgc
     1621 gcgcgtgggc gccatcgttg aggcgcccgc tctctacccg cacctcagcg cagaccgaaa
     1681 cctggaggtc ttctcgttgc tctcaggagg cgcgtcgccg gagcggcgcg ccgaagtcct
     1741 ccgcctggtg ggcctggagg gccggggaca cgaccgggtg aagagctatt cgcacgggat
     1801 gaagcagcgt ctggctatcg cccaggcgct ccttccccac cccgaactca tcctgctgga
     1861 tgaaccgacg aacggcctcg atcccggagg gatggttgaa gtgcgccagc tgctccggca
     1921 tctcgccagc caggagggaa tcaccctctt cctttccagc cacctgctct ctgaagtaga
     1981 gcaggtgtgc aatcgtgtcg cggtgatcga tcaggggcac gttctggtgc aaggagaggt
     2041 gagggagttg cttcgggagc aaacgttcct ccagatcacc gccgagccgg tggagctggc
     2101 tcgccaaatc ctggaaaccc tggactacgt acgccaggtg atcgtgcagg agggcaccct
     2161 gcgcatcgac gcgccgctcg attccgccgc cgcgatcaac gccgcgcttg tgcacggtgg
     2221 atgccgtgtg agcgcgctcg tacccggccg gcaatcgctc gaggagttct acctcggcct
     2281 cctggagaaa cgctcatgac ccacctcctg cgtgtggaga tcctgaagct gtcgcgccgg
     2341 ccgcgcacct atctcggcta cgccggcatc ctgctcctgc tggtgccgat gctcctgggg
     2401 ctcctctacg gccagcccga tcgccacctg cgccatgaaa tcggccccga gttccatgtc
     2461 gtcggcacgt tcgtcaacgc gctcttcatg gcccggcgca tgatggagcc gtgcctttgg
     2521 ttcttcctcc ccctcttcat cgccatggtg gcgggcgacc tggtggccgg cgaagccgcc
     2581 gagggcactc tccgcgcgct gcttgtgcgc ccggtgcgcc gcgtgccggt ccttctggcc
     2641 aagttcactg tcgccgtgct ccatgccacc gcgctcacct acttcctggg cctttccgca
     2701 ttgggcctgg gcaccctctt cttcggttgg ggcgacctca tcctcatcga gcacggcatc
     2761 tacgtcctgg gatccggcga ggcgcttgcg cgactggcgg ccgcctatac cgccgccatt
     2821 cctggcatgg tggttgtggc cgcgctcgca ctcttcctct cagtgcttgt cgacaactcc
     2881 aacggcgcga tcatcgccac gatgatgctc ctcttcgtct cgttcaccct cggcggtctc
     2941 ccctatttcg aggcgattca accctacctc ttcaccacgc acatgggcgt ctggagctcg
     3001 ctcttcgaag acccgatccc ctggtccgaa atcgcccgat ccatggcgtg gcttgccgca
     3061 tactgcatcg ctttcgtggg tgcggccgcg ctcgtcttct cgcgcaagga tatcctctcg
     3121 taggcgctct catccaggcg ggtgctctgg aagcgggtgc tctggaagga gccccatcgc
     3181 caggcgccga agttgcatcg taccagacct cacacannnn nnnnnngccc ggcggggctt
     3241 ccgagggcag gatgcgcgcc ggggaagagc ggcggcatcc agcccgctgg cgtccccgga
     3301 ccggccttca ccgccatcct gcccggccat tccggccggg tcgcacggtc gcttcaccgg
     3361 cgggctccgc cgcctgcgaa ccgagaagag gagtgtgcac gtggctactg caaaacggct
     3421 tccactggaa acgaaagcaa cggcgaagac gccggtcatt ggcgtctttg cgacgggaga
     3481 tccccgcatt gaccaggcat cgcgcgaccg cgcggtaaac atcgtcgaga tggccgcgaa
     3541 cgtcatcgcc ggcggcgtga agctacccga cggcacctcg gtgccggtcg tctactcccc
     3601 cgttcttgtg gatggcgagc gcgaggccga tattgttgcg cgccagttcc aggccgaggg
     3661 cgtcgatatc ctcgtctgcg tgcccgacac gtgggccttc ccgcagctgg gcctcatctc
     3721 actggtcagc cagttcccgg aagagacgcc cctcaacctg acgtgcggga atagcggccc
     3781 aaggccgggt gtggttctga cgcacgcggc gaacggcgcg ttctcgcagt atggccggct
     3841 cgttcacatc aacgtgggca cctggcccga taccggccag aacccccaga tgacccccgc
     3901 gaccgcgaag agcctgatcg attgggtcta tgccgccgtc acgcggcagg cgatccgcgg
     3961 ccggcgcgtg gccattttcg gccacgactc catgggcatg gagaccgccc tcgcccatgt
     4021 catcccgacg cggcagacct tcggcctgga gattacccgc ctcgatatga agctcctggc
     4081 cgatatgctt cagaagaagg cgtacgacgc gaaggagctg gaagccctgc gcgcctggct
     4141 gtttgagcac gtcggcccgc ggattgagat acgcgacgat gcagaccgcg agcgcctgga
     4201 tcagggcctc gcgatgtacc tgatcactcg cgacctgctc aaggacctca acgccgttgg
     4261 tggcgggttc atgaaccagc tggagtgggg ttccgaccgg cgtggcatcc cgcttccggt
     4321 ggccgatgtc atggagtcgc tcttcaactc cagcttcgac cacaacggcc ccaagccgcc
     4381 gctcccgttc gccacggaag ccgataccca ggctcttctc accatgctcg ccttcaccta
     4441 cctcggtggg gggaccccac ccctctttat ggacttccgc aaggtgtggg agccgtggga
     4501 gatcaaccag ctcgcagata gcctgggcat gggcgatgtg gagccggacg cgctctggcg
     4561 ccgcaagggc ctggtggatg gcaacaactc cggcagcgcc tccttcgatt gggccggcaa
     4621 gccgggcgac agcgtgaagg agctcctctt gcgcgtctcg atgccgcagg ccgatcccga
     4681 ttacttcccg ggcgggggca attcggtcac cttcatcacc ccgggcggga tcgaggggat
     4741 cgccggccgc cttggctaca atacgctcag cggcctcttc tccctcatct gggacgaggc
     4801 ggtcacggtg gacctacccg agaagctcgc tcacgccgtg gcaaacacct cgaaccccac
     4861 ctggccccat acctgggtgg tgcccaagta cgccacgatg ggcgagtaca agcagttcgc
     4921 gccagccaat cacttccata tgaactgggg tctcaacccg gcgcgcctgg agtactggat
     4981 ggacctggcg aacgtgctct ccgcgacccc gtggcaagac cgcccctcct atatcgaggg
     5041 ggtggaccgg cccacgccgc tgctctacat ccagaacggt ggcgaagcga cggcgaagat
     5101 gcggcgaaag cggtagctcc tgtctgtggt gggcagggct cgcaggtggt cctgcccacc
     5161 atgcatcaag ctggcggcgc tccggacggc aggggcggat gtgcctctcc agccggcggc
     5221 atctcgccgg gtgctcctgc ttccgatctc tgggccagcg catccagaaa ccgctggtga
     5281 gcttcacgaa cgatctcgag cgcctcttcc ggtgagtcca ccacctgcac cagttccata
     5341 tcctcggggc tcacgaagcc gcagcgcacc atctcccggt tcatccagtc gatgagtccg
     5401 ccccataggc gtttccctag cagcaccacc gggcgctcct caatcaagcc tacctggagc
     5461 agctgccaca cgtaggccaa ctccaacaag gtgccgattc cgcccgaggc gacgatgacg
     5521 gcgtgagtga ggcgcatgaa ctcatccagt cgcagggaga agcgcttgtg ggagctcttc
     5581 acatccaggt gcttgttggc catctcggcc agggtgggaa gctcgatggt gacgccatac
     5641 gaggtactca cgccggtgcg cgcctcatgt accccgcggt tggcagcctc catcagcccg
     5701 gggccgccgc ccgtcacgat atcgatcccc tcgtaagcca gctgtctggc caggttgaga
     5761 accaggcggt aggtttcatc ggtgggcctg atccgggcag acccgaagat gcacacgcga
     5821 aagcgggtgt tctccacgtt gcgcagcagc gtatccagcg tcaggtactc ctcgagcacc
     5881 cgttggtatc gctcccggat ctcatccact cttctcttct cccgtcccgg tctggcgctc
     5941 cggcagcagc acgacgagga gcgccgccac gcccgcgagc agcccgcacg cgcgaaacgc
     6001 ggccgggatg ccccacgcct gagccagatc cccggccagg cgagagccca gcaccccgcc
     6061 caggccgccg acgatcgcgg cgtgcagcgt ctggccgctg gcgcggaaac gctctggcac
     6121 gctcgcattg atgaacgtca gcgacgcgat gtgcgtggcg ccaaacgcca gtgcatgcaa
     6181 gccctgggcc agagccacgg ccccaaccgg gaggaaaagt gagaagagaa tcaggcgcag
     6241 cgccccgccc gccatcccga gcatcagcat gccgcacacg cccatcctct gaacaagccg
     6301 ccacgcgaac accatgaaga ccacttctgc cgccacccca aggctccaga agacgccctt
     6361 catgctgtca ctcacgccga gcccatccag gtagatactc aggaagacgt agtaggagcg
     6421 tagcgccgtg ttgcaaagga acgttgccgc caagaaaagg agaaagcccc ggtcacgaaa
     6481 cagcgcgccg aggtcgcctc ggtaggtcgc cactgcctgg ggcgcctctc cccgcggaag
     6541 cgagagcacc cacgccgcgc ctagaacccg cacaatccca aacgccacaa atgtggcgac
     6601 gagatctccc ccaccctttg gcgccagcgg cccgcctccg aggaaaaggc cagacaccag
     6661 caacgtgaca atgaaaccga gcgagcccca cagccggatc cggccgtagt ctccacccgc
     6721 gcgcggcagc agctccaggg caatcgcatc cgtgagcggg atgcacggcg caaacgagaa
     6781 gttgaagacg gcctggatga gcagcgcgct gccgagggag tctgcaagca ggaacagggg
     6841 ataagttgcc ccggaggcaa gaaagaggag cgcgagaggg atccgcttgt cacctgcccg
     6901 gtcggtcacc atgccccaca ggggcggtga gagcacggcg aggagcgccg ctagggcgtt
     6961 cagggtgccg atctcggact cggtgaagcc acaccgcttg aaatggaggt tctgatacgt
     7021 ggtcgcgatt gcgaaggggg caaaaaggag aagatagtgg atctgaagcc gcacccgaag
     7081 cgcgcccggc ttcaacgttg ccttggcgat cgtcgcatcc cctccgccgc cgaacaacga
     7141 gcggcgtggg cagggcagac gccaaacccg ccggctcctg ccttgcccac gccgtcgttc
     7201 ttacgctact tcgcgtacgc cttggcgacc ttcaggatcg ccgcagcgat ctgctccata
     7261 tgcttctcct cgtacaccgg gtgaaccggg acgaagaagg tgttcttctc caggtaggcc
     7321 gcgttcgggc agaagacctt ctcgtactga actgcctcgg ggcgcgccga cgggtcaccg
     7381 aacgggtagt tcagccggcc aaagccgcgc cgctcctgat acgccttctc ctggtagcac
     7441 tgcggccaga gcaccgggcc ggcaggaatg ccctctgcac ccagcgccgc cgtgaactgc
     7501 ttgatgtcgc aggtgaggcg gtccatccgc atcgagatcg gatagagcca gtaggagttc
     7561 cggcgctccg gggtatccaa cgggagggtt tcgatctcct cgcacgactg caggaggcgg
     7621 gtcaacatct cgccgtttct gcggcggttc ttgaggttcc agttatccat cttggacaac
     7681 tgcaccaacc cgatggccga ctgcatctcg gtcatgcggt agttgaagcc gaccatgttg
     7741 tgaatgtatg gcagcttcgc ctcgagctcc aggagcttca ggcgctgggc tacgtcatag
     7801 ccgtggtcgc ggaacgagcg gcagagccac gccacatctt catcgtcggt gaccacgcag
     7861 ccaccctcgc caccggtcgt gaagtgcttg ctctggcaga acgagaaggc gccaatgtcg
     7921 ccgatggtgc cggcgcgctt ccccttatac tccgcgccgt gcgcctgggc gcagtcctca
     7981 atgacgaaga ggttgtgctt cttcgcgatc tcgaggatcg ggcccatgtt cgacatgatg
     8041 ccatagagat ggaccgtcag gatcgcgcgc gtgcgatcgc tgatcgcggc ctcaaccgaa
     8101 gccgggtcca gcgtgtggct ctccttctcc acgtcggcga agacgggaat cgcgcccgcc
     8161 tggcagaccg agaaggagga agcaatgaac gtataggaag gaacgatcac ctcgtcgcca
     8221 gggccgatcc cgaggccggc gagtgccgtg tgcaatgcgc tggttccgtt gctcgtcgaa
     8281 atgccgaacc gggcgccaca ccactcggcg aacttctgct cgaactgcat cccgagaggt
     8341 ccggtccagt agttcacctt gccattccga agcggctcca tcgccgcctg gatcgtctcc
     8401 tcatcgaacc agggccatgc aggaaacgga tctttagtga cgggtgtacc accctcaatc
     8461 gcgagtttct ccg
//
LOCUS       DAOSCV010000046         8450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5474_length_8450_cov_3.630375, whole genome shotgun sequence.
ACCESSION   DAOSCV010000046 DAOSCV010000000
VERSION     DAOSCV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5474_length_8450_cov_3.630375"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLY56_02075"
     CDS             complement(<1..201)
                     /locus_tag="PLY56_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20297.1"
                     /translation="MHIPRILTRTWLAALMAAPTMVTGLSTAHASPSGLNNIPTADVA
                     PNGVPVVQLYTTLAEGSSSHATL"
     gene            complement(275..1378)
                     /locus_tag="PLY56_02080"
     CDS             complement(275..1378)
                     /locus_tag="PLY56_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ20298.1"
                     /translation="MSDTRMILVGGFLGAGKTTLLTRAAQRLASQGKLVAVVANDQAA
                     NLVDTGTLRSAGLYVGEVAGGCFCCRFDQFLDECGKLARESKAEVILAEPVGSCTDIS
                     ATILQPMKDLLTEKYRLAPFTVLTDPYRLREVFSEGVSSNFPDSVTYIFRKQVEEADL
                     IAINKIDLLTEAEIAELKAMVAKHAPGTPILTLSALTGEGVDAWLDCVLSETSAGTRI
                     TEVDYDTYADGEAVLGWLNATAQLHAAGQPDWDGFCLSLMEQMQGEFRARSAEVAHVK
                     LLLATTGGRITVNLTATGGKPSLNGSAERTVQDALLVINARVRMEPDELQAVVERSVQ
                     AACDGQVEGRITSIASFKPGRPQPTHRYAAVVS"
     gene            complement(1425..2255)
                     /locus_tag="PLY56_02085"
     CDS             complement(1425..2255)
                     /locus_tag="PLY56_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HOJ20299.1"
                     /translation="MKRSLGQECAGEVLGTFILVFFGVGSVHAAVLTGAQSGLWQVAI
                     VWGVAISLAIYATGAVSGAHMNPAMTIAFAAWRGFPWRKVLPYVLSQLVGAIAGAAVL
                     YGLFHNVTAHFEAAKQLVRGAAGSELSAMTYGEYFPNPALVASGAMAVESVTHLQAML
                     AEAIGTAFLAFFVFAVVDDRNPGRPNGTLFALFIGLTVSIVISIVAPLTQAGLNPARD
                     FGPRLFSFFAGWGEIAIPGPRGGFFTVYILAPIVGALVGSALYERVVRPALPPAEANA
                     "
     gene            2961..3608
                     /locus_tag="PLY56_02090"
     CDS             2961..3608
                     /locus_tag="PLY56_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20300.1"
                     /translation="MEKAPPLTEVRADPLAAEPLTAGHPAPSDQSTASPLTASSPGTP
                     KSSSSVAARPPAPGGLRLPTTAGIPPPSITGAAPRRIPAEAPRTPPVTSPGSLGFHEE
                     PAGERNPLLVMINCEDDTMVVILSGQKRYRRVLMPKATIHLQIEPGEYQVRVHATKSS
                     PNSGRGVFREYHRYTARWVSVPTHQARPLRLGELSPESREAASEETPVIPVPEEP"
     gene            3959..4990
                     /locus_tag="PLY56_02095"
     CDS             3959..4990
                     /locus_tag="PLY56_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ20301.1"
                     /translation="MIIAVLALLWATATEAGEFSPMAYYSLNPHDDIVIGGTAALRGE
                     RTLAMASLGYSWAQGEPRGRLRYIHDHGLGSRLGYGVQFFHEVQARSDLVPLFQTGYY
                     EGHTGVGLTGFWRMGKGKLGTLSLIQEKVWALEERGTLLPVGEDRIHAVRLGFQQDSR
                     DDRMNPRSGGRVEASATQAIRVFGGTRSYFSSRLSWSRYEPLGPEGSLGWRLHGALTS
                     GTLPVQRALWLAGEGVRGYDFLSDRGNVAGVANLEMRWPLLRDGERSVGFLGAIRQLQ
                     VVTFADVGALQTAESGWVMRGGIGIGLRAPVVALGRVPMVLRLDAAQGLTRDGKLNSY
                     VLLTAPELF"
     gene            complement(5014..5274)
                     /locus_tag="PLY56_02100"
     CDS             complement(5014..5274)
                     /locus_tag="PLY56_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOJ20302.1"
                     /translation="MSILAWIIVGLIAGLIARALVPGEEPGGLLADLLVGILGAVVGG
                     WIFNAIGIGGVTGFNLWSILVATIGAALLLFIYHAVTGRRTA"
     gene            5358..6209
                     /locus_tag="PLY56_02105"
     CDS             5358..6209
                     /locus_tag="PLY56_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ20303.1"
                     /translation="MRVEIWGARGSIAAPGELTARYGGNTACVSVRFDSGEVLILDAG
                     TGIRRLGNVLARQQPVTAHLFLSHCHWDHIQGFPFFKPAYLASTRLRIYSHHMPRQQL
                     RKTLTDQMVGAYFPLEFSGLSASIEFVKMDEHSLRVGPAEITAVETNHPGGGVGFRIA
                     AEGRVFVYLTDNELADGNHERFIDFCRGADLLIHDAQYLPEELESHQGWGHSSYLEVL
                     DLARAAGVREVVLFHHDPDRTDAEIDAIVHACHEEMRQRGDRFECFAASEGMVLTILT
                     AGEPDRA"
     gene            6206..7339
                     /locus_tag="PLY56_02110"
     CDS             6206..7339
                     /locus_tag="PLY56_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HOJ20304.1"
                     /translation="MSRPVERQPPAAPRRPDRRWVVVGWALLLVAAGLQRWAPAPIVE
                     RLYSRGLYPWIAALLSLVNEWAGFSLAEAGAIILPPALLFALVWSAVRARRMGVRWGR
                     WLAGTTGRGIAAAGWILFAFSLLWGLNYRRVPLSERLGIPNGGTAEELAATARWLAGE
                     LARHAPPTKHRDRPAGHPYPWEELAAKVEDAYEALPVAGEGFPRRLGPAKPVRASGIL
                     SRLGLSGIFIPFTGEPNVNRLLPHPSLLAAVAHEKAHQRGITHESEASFAGFLALYQS
                     SDPYLRYCGLLEATGSVLGALARTDPEAFAEVARAIPATARADWARSREFWARYRGPA
                     QRVSRRVNDLYLRVNRVPGGVQSYGAVVDLLVGYYLAKASQEP"
     gene            complement(7551..>8450)
                     /locus_tag="PLY56_02115"
     CDS             complement(7551..>8450)
                     /locus_tag="PLY56_02115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ20305.1"
                     /translation="LEGAQFGWLRGLSPELDAGKLREVRAHADELGLYTHVSVPTCNP
                     HLVQGSIDDHLAEMARQIELAAQCGWHELHSSLGNGPERYEHPVRWPQHLADSADLIR
                     RLGPVLRAHQSRINLETHGDTTTFELVRLIEDVGPDIAGICLDTANVLCHAEDPVMAA
                     RRAAPYTHLTHTKDAIIYFSETGYTRQGRAPGQGCLDWEATLPILGEFSPDLPLSIED
                     HKWLFPAHIFDRHWLALHPDLTPHELAQVVKLAAECEKRITRGELEQPEEYEAIPYRD
                     QMMERLRFGRDYLLGVLERRGLR"
BASE COUNT         1444 a         2798 c         2797 g         1411 t
ORIGIN      
        1 cagggtggcg tgggaggagc tgccttcggc cagcgtcgtg tagagctgca cgacgggcac
       61 gccgttagga gccacatcgg cagtcgggat gttgttcagg cccgatggcg aggcatgggc
      121 ggtggagagc cctgtcacca tcgtgggggc ggccatcagg gcagccagcc acgttctggt
      181 gaggatacgg gggatgtgca ttcgggtgtc tccaagggaa cagcaggcgt ggtgggtaac
      241 cgatctccca ccacgccttg agctgggcta ccggttagga aacgactgcg gcatagcgat
      301 gcgtcggctg cggtcggcca ggcttgaagc tggcaatgct cgtaatccgg ccttccacct
      361 gcccatcgca cgcggcctga acgctgcgct ccaccacggc ctgcaactca tcgggctcca
      421 tgcggacgcg cgcgttgatc accaggaggg catcctgaac cgttcgctcc gcgcttccgt
      481 tgaggcttgg cttgccgccc gtcgccgtga ggttgacggt gatccggccg ccggtggtgg
      541 ccaggagcag cttcacgtgc gccacttccg ccgagcgcgc gcggaactcg ccctgcatct
      601 gttccatcag cgacaggcag aagccatccc aatcgggctg gccggcggcg tgcaactgcg
      661 ccgtcgcatt gagccagccg aggacggcct cgccatcggc ataggtgtcg tagtccacct
      721 cggtgatgcg agtgccggcg gaggtctcgc tcagcacgca gtcgagccac gcatccacgc
      781 cctcgccggt gagggcagac agggtaagga tgggagtgcc aggcgcatgc ttggcgacca
      841 tcgccttgag ctcggcgatc tccgcttcgg tgaggaggtc gatcttgttg atggcgataa
      901 gatcggcctc ttcgacctgc ttgcggaaga tgtaggtcac gctgtccggg aagttggacg
      961 acacgccttc gctgaaaact tcgcgcaggc gatagggatc ggtcaggacg gtaaagggcg
     1021 ccaggcggta cttctcagtg aggaggtcct tcatcggctg gaggatggtg gcggagatgt
     1081 cagtgcagct tcccacgggt tcggccagga tgacctctgc cttcgactcc cgggccagct
     1141 tgccgcactc gtctagaaac tggtcgaagc gacagcagaa gcagccgcca gcgacctccc
     1201 caacgtacag gccggccgaa cgcagggtgc cggtatccac caggttcgcg gcctggtcgt
     1261 ttgccaccac agccaccagc ttgccctggc tggcaaggcg ctgggctgcc cgagtgagga
     1321 gcgtggtctt gcccgctcca agaaacccac ccaccaggat catccgtgtg tcgctcattc
     1381 tcttactcct ttcaggatca gcagatacgc acaggagcag gagcctacgc gttcgcctcg
     1441 gccggaggga gcgccgggcg gaccacccgc tcgtagagag cactgccgac gagtgcgccc
     1501 acgatgggag ccaggatgta gacggtgaag aagcctcccc tggggccggg gatggcaatc
     1561 tcgccccaac ccgcgaagaa ggagaagagg cgcggcccga agtcacgcgc cgggttgagg
     1621 ccggcctggg tcaggggcgc cacgatagag atcacgatag agaccgtcag gccgatgaaa
     1681 agcgcgaaga gcgtgccatt gggccggccg gggttgcggt catccacaac ggcaaagacg
     1741 aagaaggcga ggaatgcggt gccaatcgcc tcggccagca tcgcctgaag gtgcgtgacc
     1801 gattccaccg ccatggcgcc actggcgacc aaggcggggt ttgggaagta ctcgccgtag
     1861 gtcatggcgg aaagctcgct gccagcagca ccgcgcacca gctgcttcgc ggcctcgaag
     1921 tgcgcggtca cattgtggaa caggccgtag agtacggcag cgccggcgat ggcccccacg
     1981 agttgcgaca acacgtaggg aagcaccttg cgccacggga agccgcgcca ggcggcgaac
     2041 gcgatggtca tggctgggtt catatgggcg ccgctcaccg cgccggtggc gtagatggcg
     2101 agagagatag ccacccccca aacgatcgcg acctgccaca gaccggattg ggcgccggtg
     2161 agcacggcgg cgtgaaccga accgacgcca aagaaaacga ggatgaacgt gccgaggact
     2221 tcgcccgcac attcctgacc gagactgcgc ttcatggggt ggatcctcct gtttcgttga
     2281 tctcgcggtg atacccattc gttgcgccga tgcgacaaca gctccagtct tgcacggctt
     2341 cctgctactc gtcgggctgg cagcggtaaa gtggcatgtc tggcgtgccc tcatcttata
     2401 ggttattcca tcccgaggtc aacgctctcc ggggggtgaa ggcattaagc gtctgttaaa
     2461 cccgcgttaa gaggcgcact cacggggccg ttgtaccagt gattcaaaaa gggttgaaac
     2521 gtcgagccca tcatgccgga ccagcgcacg ccatcagatg atcgtgccgc cggatcggct
     2581 gtgccagacc aacacttcaa aaactctttg atagttttac ccgggggaga cggaggcagg
     2641 aaaaggcccg ggagtcaggc aatccgggtc tgcccccttc ctccgtggta tcgatggcgg
     2701 caagagcacg ttgacacttc cccccctcga ccctataatg ggacgtgaaa ggcacggaac
     2761 tccaatgcga gcggtgtccc tgaaggtggt tggaggggtg gcggcggtga tcgtcgtggg
     2821 actgggcatt tcgctggtca tccgtgacca gaacgatgca cgcggggggg cttgtctcgc
     2881 cccggtctcg gcccgcgcgc tcggagcagg cgctggtact ccagcctgac cgggtgccgg
     2941 gtcacgtggc gcggctaccg gtggagaaag ctccaccgct caccgaagtg cgcgcggatc
     3001 cactcgccgc cgagccgctc acggccggcc atccagctcc ttccgatcag tccaccgcgt
     3061 ctccgctcac cgcgtcgtct ccaggcactc ccaagagctc ctcttccgtc gccgcgcgcc
     3121 ctcccgcgcc gggcgggcta cgcctcccca ccacggccgg aatccccccg ccatccatca
     3181 ccggcgccgc cccgcgccgc atccctgccg aggctccgcg cacgcctccg gtcacatccc
     3241 ccggctcgct cggattccac gaggagccgg caggagagcg caacccgctg cttgtgatga
     3301 tcaactgtga ggatgacacg atggtggtga tcctatccgg gcagaagcgc taccggcgcg
     3361 tgctgatgcc caaggcaacc atccacctcc agatagaacc aggcgagtat caagttcgag
     3421 tgcacgcgac gaaaagctcg cctaactccg gacgcggcgt ctttcgcgaa taccatcgct
     3481 acaccgcacg ctgggtgagc gtacccacgc atcaggctcg cccgcttcgc cttggtgaac
     3541 tgagcccgga gtcgcgggag gcggccagcg aggagactcc cgtgatcccg gtgcccgaag
     3601 agccctagcg cctgccctat agcgcctcgc tcctcaagtt tggccagttc ctgccgataa
     3661 ttatggcggc agggtctccc tttgccgatg cctggaaaga gccagacggt agggcactct
     3721 tcttcatggc tcctctcttc caggaaccag atggaacgga gtctatcggg cgtgatgcgc
     3781 cggcgggcac ggctgctcac cgcgcatggc ggagcgcctt gcccgagatc aggaccggat
     3841 ggaataaacc ccgagaggag gacgggagct gcagaagcct ctcccttgcc ggcgccgcgc
     3901 acgaagcccg cggggcggac aagaggtctc tgcatcccga atctgcgtga gaccaatatt
     3961 gatcatcgcg gtactggcgc tgttgtgggc caccgccacc gaagcaggcg agttctcgcc
     4021 gatggcctac tacagcctca atccgcacga cgatatcgtg atcggaggca ccgccgcgct
     4081 gcggggcgag cgcacgttgg ccatggcgag tctgggctac tcgtgggcac agggcgaacc
     4141 tcgcggccgc ctgcgctaca tccacgacca cggtctgggc agccggcttg gctacggcgt
     4201 acagttcttc cacgaggtgc aggcacgctc agatctcgtg cccctctttc agacgggcta
     4261 ctacgagggc cacacgggcg taggcctcac cgggttctgg cggatgggca aaggaaagct
     4321 gggcaccctc tcgctcatcc aggagaaggt atgggccctg gaggagcgtg gcacgctgct
     4381 tcccgtcggc gaagaccgga tccacgccgt gcgtctgggc ttccagcaag actcgcgtga
     4441 tgaccggatg aatccgcgct cgggcggacg ggtcgaggca tcggcaacgc aggcgatacg
     4501 cgtctttggc ggcacgcgca gctacttctc atcccggctc tcctggtcgc gatacgagcc
     4561 cttggggcca gagggctcac ttggatggcg cctgcatggg gcgctgactt caggcacact
     4621 ccctgtgcag cgagcgctct ggctggccgg cgagggcgtg cgcggctacg actttctgag
     4681 cgataggggc aatgtcgccg gcgtcgcgaa cctggagatg cgctggccac tccttcgtga
     4741 tggggagagg agcgtgggct ttctcggcgc gatccggcaa cttcaggtcg tgactttcgc
     4801 ggatgtgggc gctctccaga ccgccgaatc cggatgggtg atgcgcggag gaatcggcat
     4861 tggcctgcgc gctcccgtgg tcgcgctggg ccgtgtgccg atggtcctcc gcctggatgc
     4921 cgcccagggg ctcacccggg acggcaagtt gaacagctac gttctactga cggcgccgga
     4981 actcttctga gccggggcat aaccatgcgt ttgtcaggca gtcctccggc cggttacggc
     5041 atggtagatg aagagcagca gggccgcgcc gatggtggcg accagaatgc tccacaggtt
     5101 gaagccagtg acgccgccga ttccaatcgc gttgaagatc cacccaccga cgacagcgcc
     5161 gaggataccc acgagcagat cggccagcag gccgccgggc tcctcgccgg gaaccagggc
     5221 gcgcgcgatg agaccggcga tcagtcccac gatgatccat gcaagtatcg acatcgccgt
     5281 tacctcccgc cccactcttc ccgcgaggcc agcggcccaa accgagtgag ggcaacgcgc
     5341 gcgatggggg agcagctatg agagttgaga tctggggcgc ccgcggctcg atcgcggcgc
     5401 caggagagct caccgcccgc tacggcggca ataccgcctg cgtttccgtt cgcttcgaca
     5461 gcggcgaggt gctcatcctg gatgccggca ccggcatccg ccggctcggc aacgtgctcg
     5521 cgcgccagca gcctgtcacc gcgcacctct tcctcagcca ctgtcattgg gatcacatcc
     5581 agggctttcc ctttttcaag cccgcctacc tggccagcac ccgcctgcga atctacagcc
     5641 atcacatgcc gcgccagcaa ctccgcaaga cgctcaccga tcagatggtg ggcgcctact
     5701 tccccttgga gttctccggg ctcagcgcct cgattgagtt cgtcaagatg gacgagcact
     5761 cgctccgcgt tggcccggcg gagatcaccg cggtcgagac caaccacccg ggcgggggcg
     5821 tcggctttcg gatcgccgcc gagggccggg tctttgtcta cctgacagat aacgagctcg
     5881 ccgacggcaa ccacgagcga ttcatcgact tctgccgcgg ggccgacctc ctgatccacg
     5941 acgcccagta cctgccggag gagctggaat cgcaccaggg gtggggccat tcctcctatc
     6001 tggaggtgct cgatctcgcc cgcgccgccg gagtccgcga ggtcgtcctc ttccatcacg
     6061 acccggaccg caccgacgcc gagatcgacg cgatcgtcca cgcctgccac gaggagatgc
     6121 gccagcgggg cgaccgcttc gagtgctttg ccgcttcgga gggaatggtc ctgaccatcc
     6181 tcaccgctgg ggagccagat cgcgcatgag ccggcccgtg gagcgccagc cgcccgccgc
     6241 gccgaggcgc ccggatcgcc gctgggtcgt tgtcggatgg gcgctccttc tggtggccgc
     6301 cgggttacaa cggtgggcgc ctgcgcccat cgtggaacgc ctctattcgc ggggcctcta
     6361 tccctggatc gcggcgctcc tatcgctcgt gaatgagtgg gccgggttct ccctggccga
     6421 ggccggcgcc atcatcctcc cccctgccct cctcttcgcc ctcgtgtgga gcgcagtgcg
     6481 cgccagaagg atgggggtga ggtgggggcg gtggctcgcg ggcacgacgg ggcgcgggat
     6541 cgccgcggct ggctggatcc tcttcgcctt ctcgctgctg tggggcctca actatcgccg
     6601 ggtgccgctc tcggagcggc tgggaatccc taacggtggc actgccgagg agctggcggc
     6661 caccgcgcgc tggctcgcgg gagagctcgc ccggcacgcg cccccgacga aacaccgcga
     6721 ccgccctgcg ggccacccct acccgtggga ggagctggcg gctaaggtgg aggacgccta
     6781 cgaagctctc ccggtggccg gcgaaggctt cccgcggcgc ctcggacccg ccaagccggt
     6841 ccgtgcctcc ggcatcctct cgcgcctggg cctctcggga atcttcatcc ccttcacggg
     6901 cgagccaaac gtcaaccggc tgctgcccca ccccagtctc ctcgcggccg tggcccatga
     6961 gaaggcgcac cagcgaggca tcacccacga gagcgaggca agcttcgccg gcttcctggc
     7021 gctctatcag tcttcagacc cctacctgcg gtattgcggc ctcctggaag ccaccgggag
     7081 cgtgctggga gctttggcgc gcacagaccc cgaggcgttc gccgaggtgg cccgcgcgat
     7141 tcccgccacc gcgcgggccg attgggcacg cagccgcgag ttctgggccc ggtatcgcgg
     7201 gccggcccag cgcgtgtcgc gccgcgtcaa cgacctctac ctgcgagtca accgcgtgcc
     7261 aggcggagtg cagagttacg gagcagtggt cgatctcctg gtgggctact acctggcaaa
     7321 agcaagccag gaaccatagc ccgaagtatt cgtcagaata cttcaagaga agactcttgc
     7381 ctggtggcac accgttgccg ccgcaagcgt ccggaagcag tgcccacgaa tccgcgccac
     7441 gcctggcaga gagacttctt ttcattattc tgacaaataa tgcgggatag cgccgcgcca
     7501 gcaagcggtg gctcctgcac aagttgcccg tgggggctcc actccgagtg ctatcgcaag
     7561 ccccggcgct ccagcacgcc cagaaggtag tcgcgcccga agcgcaggcg ctccatcatc
     7621 tggtcgcgat aggggattgc ttcatactcc tcgggctgct ccagctcgcc cctggtgatc
     7681 cgcttctcgc attccgccgc gagcttcacc acctgcgcca gctcgtgcgg ggtgagatcg
     7741 ggatggagcg cgagccagtg ccggtcgaag atgtgggcgg ggaagagcca cttgtgatcc
     7801 tcgatggaga gcggcaggtc gggtgagaac tcgccgagga tcggcagggt cgcctcccaa
     7861 tcgaggcagc cttggccggg agcgcgccct tgccgggtgt aacccgtctc ggagaagtag
     7921 atgatcgcgt ctttcgtgtg ggtgaggtgc gtgtagggtg ccgcacgccg ggcagccatc
     7981 accgggtcct cggcatggca gaggacgttc gccgtatcca ggcagatgcc ggcgatatcc
     8041 gggcccacat cctcgatgag gcggaccagc tcgaacgtgg tcgtatcccc gtgagtctcc
     8101 aagttgatcc ggctctggtg cgcgcggagc accggcccga gccgtcggat cagatccgcg
     8161 gaatcggcga ggtgctgggg ccagcgcacg gggtgctcgt aacgctcggg gccgttccca
     8221 agcgaggagt ggagctcgtg ccacccgcac tgggcggcca gctcgatctg ccgggccatc
     8281 tccgccaggt ggtcgtcgat gctcccctgc accaggtgcg ggttgcacgt gggcaccgag
     8341 acgtgcgtgt agagaccaag ctcatccgcg tgggcgcgta cttcccgcag cttccccgcg
     8401 tcgagctccg ggctgaggcc gcgtagccag ccaaactgcg cgccctccag
//
LOCUS       DAOSCV010000047         8448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5476_length_8448_cov_3.146789, whole genome shotgun sequence.
ACCESSION   DAOSCV010000047 DAOSCV010000000
VERSION     DAOSCV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5476_length_8448_cov_3.146789"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLY56_02120"
     CDS             complement(<1..439)
                     /locus_tag="PLY56_02120"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ20306.1"
                     /translation="MLTTEAWIRQLREEITSLREEGADVDAAERQLDAIAAGNLDPSH
                     LPALQASLQTSVTRSDFPYAEPVALEEIQMLRPEGPRSLPVWLPPDLLFDRIYGGWLG
                     RAAGCLLGKPVEGWKKEEIEETLRFCNAWPLSDYFPPVPDNDRG"
     gene            827..1957
                     /locus_tag="PLY56_02125"
     CDS             827..1957
                     /locus_tag="PLY56_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20307.1"
                     /translation="MKRNRKIRIGVVGGRFGATFQWHEHPGCIVQAVCEGREDRREEL
                     RRVYRCDKAYSTLDELLQDKEVDAVALFTAAPDHARHSVACLNAGKHVMSAVPAATTL
                     EDAELLAETVRKTGLTYMMAETSYYHQVVISARKFYEEGKFGQIFYTEAEYHHAGMEP
                     LTVNPDGSKTWRHGLPPMLYPTHCTGYLVGVTRERLVSVMCLGWDSGDPFYKPNQYDN
                     PFQCQTALFKTDRGNAFRVCVFWYGAHRGTERGQWYGEKMSLFGPHPNGLGPVIVRAS
                     QETERDDAGFIRQSPTVEAYDQPKWWQTEMLPEPLRHDSGHDGSHTFLTHEFIDALQN
                     DRAPAIDIREALAMTVPGIIAHASALAGGKQMEIPTFFDRCV"
     gene            complement(1977..3446)
                     /locus_tag="PLY56_02130"
     CDS             complement(1977..3446)
                     /locus_tag="PLY56_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002618642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ20308.1"
                     /translation="MADSAHASGDKFGRDLTTGSIPRLLLAFSIPMLAGNVIQTAYSI
                     VNAIWVGKFLGKRELAAITVSFPAFFVLVAVAGGLTMASGILVAQCAGARDWRRLKDV
                     VQTSTSLAILISLLLLPLSELLTPAIMKAMQAAPDVYPMAVSYMRIFLLTLPFNIGIF
                     LIVSMLRGVGDSKTPLYFQTSALTLTAILDPILMFGWLGFPRMGLNGTAVASVVMQGL
                     CLVATFVYLHIKSHVVAPDWRRLSIDWPMAGLLVKIGFPSAAHQSLVSLSMLFVTGFV
                     NAFGENATAAFGAAMRIDQLSFLPSMTFSMAVTSMVGQNIGARRLDRVPHVFRWGMVL
                     SGAMSILATLIVLAVPQSILRIFLNDSEVIRLGVSYLRIVGSGYIFMTVIFISNGVLN
                     GAGHTLITTGSALFSLWLLRVPLASYLSNRLQHVDGVWYAILISSAATAVLGMVLYYA
                     GIWKRPVGAHHLAAGSTAAGRDGEKPDATPAQELAEERD"
     gene            complement(3813..4421)
                     /locus_tag="PLY56_02135"
     CDS             complement(3813..4421)
                     /locus_tag="PLY56_02135"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOJ20309.1"
                     /translation="MPKHTLPPLPYPYNALEPHIDEQTLHFHHDMHHKAYVDGLNKAE
                     EELAKAREAGDFSLVKHWSREAAFHGSGHILHTLFWENLGPNGGGEPQGALADAIKAN
                     FGSFDSFKAQMIAATTQVEASGWGVLAYNPMFDSLVILTAEKHQDLTQWGVVPLLVID
                     AWEHAYYLKYQNRRADFVKAVFNVINWDAVAQRYAAALRLKG"
     gene            complement(4811..5902)
                     /locus_tag="PLY56_02140"
                     /pseudo
     CDS             complement(4811..5902)
                     /locus_tag="PLY56_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
     gene            complement(5943..7253)
                     /locus_tag="PLY56_02145"
     CDS             complement(5943..7253)
                     /locus_tag="PLY56_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOJ20310.1"
                     /translation="MTEWNEHGAVVECVAPGSLAERAGVRPGDRITAINGEAFADLID
                     YRFLISDTVARLDLVRDGQPRSVQIHKEVDQGLGITFTQGVFDGIRRCRNSCVFCFLH
                     QMPRGMRKSLYIPDDDYRLSVTQGNYVPLTNVTEEDLRRILRLHLSPLFISVHATDPG
                     ARARLLRCAGNAEVLPLMRRLADAGIEMHCQIVLCPSFNDGEVLDQTLADLAALHPEV
                     LSIAVVPVGLTRYRERLTALEPVTPEGARETLARLERWRERFLAEHGTRLVFAADEFY
                     ILAGEPLPPRDAYEEFPQTEDGIGLSRLFLDELEQLRGRPAKPVPQPERVFLVTGMLA
                     HPMVEALAGELSRITGHRVEALAVANRFFGERITVTGLLTGGDILAAIRAAGPANRVY
                     VPDVLLNAGRFLDDMTLSQMAEALGVPVEAVAPSPLALARALAA"
     gene            7458..>8448
                     /locus_tag="PLY56_02150"
     CDS             7458..>8448
                     /locus_tag="PLY56_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ20311.1"
                     /translation="MKCNASLEELSAYADGELSPAEMSAIEAHLGVCDRCRKELASLC
                     GVSQAFRLAGDLEPGLSVRRSVARRLAMTEEAPEFTCARMQMSISAMLDGELSEEDAL
                     ILQAHLSTCSACASEVASLEWVSDSVRALPEVELPFGLERRIRTAIAREEQGVFSLGW
                     MRRLFQPRMAPGMATALAGATLLVGFWLGRLYPQAPGTAPVAVAPPAKPQVATRMVAP
                     NTTIRVTEQPAPLRAKPQRRPAAPVQRAPEPRRVAVRTVPAPKPALARHEEARPAPAH
                     QSAHRPESAPSPTAVATTSPLPIVRPLPEAPPPVVELPTPPAPARVSVEIAMAW"
BASE COUNT         1499 a         2756 c         2830 g         1363 t
ORIGIN      
        1 ggccgcggtc attatccggc actgggggga agtaatccga aagcggccaa gcgttgcaga
       61 aacgcagggt ctcctcgatc tcctccttct tccagccctc caccggcttg cccaggaggc
      121 accctgcggc ccgaccgagc catcccccgt agatgcggtc gaaaagaagg tccggcggga
      181 gccagacggg caggctacgc gggccctcgg ggcggagcat ctggatctcc tccagggcga
      241 ccggctcggc ataggggaag tcggaccggg tcacagaagt ttgcaagctg gcctgcagtg
      301 ccgggaggtg cgacgggtcg aggttgccgg cagcaatggc atcgagctgg cgctcggcgg
      361 cgtccacgtc ggcgccttcc tcgcgcaggc tggtgatctc ctcacgcagt tgcctgatcc
      421 acgcctcagt cgtcagcatc gcgcgtctcc ttcatccgac agcgtgcgcg gcggagaggc
      481 cgccgggctt ctggatctgg aagagggcgc tctgcgcgcg cggccacctg gcgagcgaga
      541 ctggcccgaa gccagcctgt ctcccgcggg ccggcgaaaa cgagcgccgg ggccgccatg
      601 gacggcgttg gcaacgccac gcatcgcatt gcctcaatca gacgcccctg aagcaattcg
      661 ctcgccgggt ggggatccct tctcgctgcg aggcggtgga gaggacgagc ccgccaggag
      721 tggaaatact ctcagtatca gacagatctt ccccgaggaa gaggtgaggg cgccctcatg
      781 cccgtcgcca tcgtgttcta cggggatctg ggcgagggaa taggcgatga agcgcaaccg
      841 gaagatacga atcggggtgg tagggggccg ttttggcgcg accttccagt ggcacgagca
      901 tcccgggtgc atcgtgcagg cggtgtgcga ggggagagag gaccgccgtg aagaactgcg
      961 gcgcgtttat cggtgcgaca aggcttactc cacgctggac gagctgctcc aggacaaaga
     1021 ggtggacgct gtagccctct tcacggctgc cccggaccac gcgcgccact cggtggcgtg
     1081 cctcaacgcg gggaagcacg tcatgagcgc cgtgccggcg gcgaccaccc tcgaggacgc
     1141 ggagcttctc gccgagacgg tgcgcaagac ggggctcacc tacatgatgg cggagacgag
     1201 ctactaccac caggtggtca tctccgcgcg caagttctac gaggagggaa agttcgggca
     1261 gatcttctac accgaggcgg agtatcacca tgccggcatg gagccgctca cggtcaaccc
     1321 agacggctcg aagacatggc gtcatggcct gccgccgatg ctctatccta cgcactgcac
     1381 cggctatcta gtcggggtca cgcgcgagcg cctcgtctca gtgatgtgcc tgggatggga
     1441 cagcggcgat ccgttctaca agcccaacca gtatgacaat ccgttccagt gccagacggc
     1501 gctcttcaag acggatcgtg ggaatgcctt tcgcgtgtgc gtcttctggt atggcgccca
     1561 ccgtggcacc gagcgcgggc agtggtacgg tgagaagatg agcctgttcg gcccgcatcc
     1621 caacggcctt gggccggtca ttgtgcgtgc gagccaggag acggagcgcg atgacgccgg
     1681 cttcattcgc cagtcaccga ccgtcgaggc gtacgaccag ccaaagtggt ggcagacgga
     1741 gatgctcccg gagccgctgc gccatgatag cgggcacgac ggatcgcaca ccttcctgac
     1801 gcacgagttc attgatgctc tgcagaacga ccgcgcgccc gccattgata ttcgcgaggc
     1861 gctggcgatg accgtgccgg ggatcatcgc ccatgcctca gcgctggcgg gcggaaagca
     1921 gatggagatt ccgacgttct tcgaccgctg cgtataaccg aaaccgcgga ggagcgtcag
     1981 tcgcgctcct ccgccagctc ctgagctggc gtggcgtctg gcttttcccc gtctctgcct
     2041 gcggcggtgg aacctgctgc caggtggtgc gcgccgacag gccgcttcca gatgccggcg
     2101 tagtagagca ccatgcccaa cacggcggtg gccgccgagc tgatcagaat cgcgtaccac
     2161 accccatcga cgtgttgcag gcggttggag aggtacgagg ccaggggcac gcgcagcagc
     2221 cacagcgaga agagggccga cccggtcgta atcagcgtat gccccgcgcc attgagcacc
     2281 ccgttgctga tgaagatcac cgtcatgaag atgtagccgc ttccaacgat gcggaggtag
     2341 ctgacgccga ggcgtatcac ctccgagtcg ttgaggaaga tgcgcaggat cgattggggc
     2401 acggctagca cgatgagcgt cgccaggata gacatcgccc cgctcagcac catgccccac
     2461 ctaaagacat ggggcacgcg gtccagccgg cgcgcgccga tgttctgccc gaccatcgag
     2521 gtcaccgcca tgctgaacgt catcgacggg agaaaggaga gctgatcgat gcgcatggcg
     2581 gcaccgaagg ccgctgtggc gttctcgccg aacgcgttca cgaacccggt gacgaagagc
     2641 atgctaagcg agacgagcga ctggtgcgcg gccgagggaa agccgatctt caccaaaagc
     2701 cctgccatgg gccaatcgat gctcagacgg cgccagtccg gcgcgacgac gtgcgatttg
     2761 atatggaggt agacgaaggt cgccaccagg cagagcccct gcatcaccac gctagcgacg
     2821 gccgtgccgt tgagccccat gcgcgggaag ccaagccatc cgaacatgag gatgggatcc
     2881 aaaatggccg tcagcgtcag cgcgctcgtc tggaagtaga gcggcgtctt ggagtcgccc
     2941 accccgcgga gcatcgagac gatcaggaag atgccgatgt tgaaggggag cgtcagcaga
     3001 aagatccgca tgtagctcac ggccatcgga tagacatcag gcgctgcctg catcgccttc
     3061 atgatggcag gcgtcagcaa ctcgctcagt ggcagcagga ggaggctgat gagaatcgcg
     3121 agagaggtgg aagtctggac gacgtccttc aacctgcgcc agtcgcgcgc gccggcgcac
     3181 tgagcgacca ggattccgct cgccatggtg aggccaccgg caactgcgac caggacaaag
     3241 aaggctggga agctgacggt gatggcggcc aactcgcgct tgccgagaaa ctttcccacc
     3301 cagatggcgt tgacaatgct ataggcggtc tggatcacgt taccggccag catcggtatc
     3361 gagaacgcga ggagaaggcg agggatgctc ccggtggtaa ggtcgcgccc aaacttatcg
     3421 ccggaagcat gcgccgagtc tgccatagtt ggtgccgtaa tccttcctta tccgaaccgt
     3481 cccgctccga caggcatctc cacctgaggc tgatccgtct gcccgggcga ggtgcgagca
     3541 tgcacagaat ctcattctat accttcgggc aggcatcggt caagagctac atgcggccag
     3601 gcggttgagg ggggcgagtt gcggtggagc gtggagctct tgcccgcagg aggcatccct
     3661 acggtgagga gagggggtac aaacgcgaag gcggtcttcg gggccggacg atagggtgaa
     3721 gcacccgcgg gagagctttt tcgcgcgcct aacccggcgg gtgcttcgcc ccaatattat
     3781 ggcggctcac gaatgaacgc cgcgccgtcg gattagccct tcaggcggag tgccgccgcg
     3841 tagcgctggg cgaccgcgtc ccagttgatg acgttgaaaa cggctttgac aaagtcggcg
     3901 cgtcggttct gatacttcag gtagtaggca tgctcccagg catcgatcac gagcagcggc
     3961 acgacgcccc actgcgtcag atcctggtgc ttctccgccg tcaggatgac cagcgagtcg
     4021 aacatcgggt tgtaggcgag cacgccccat ccagaggctt ccacctgcgt ggtcgcggcg
     4081 atcatctgcg ccttgaacga atcgaaagag ccgaagttgg ccttgatcgc atccgccagc
     4141 gcgccctgcg gctcgccgcc tccgttgggc cccaagttct cccagaagag cgtgtgcagg
     4201 atatgccccg agccatggaa ggccgcctcg cgcgaccagt gcttcaccag cgagaagtcg
     4261 cccgcctccc gcgccttggc cagctcttcc tccgccttgt tcaggccatc cacatacgcc
     4321 ttgtggtgca tgtcgtgatg gaaatgcagc gtctgctcgt caatgtgcgg ttccagcgcg
     4381 ttgtaagggt acggcaatgg tggcagtgtg tgcttcggca tctctcagct cctccatcaa
     4441 ctccggtggg ggcagatcct gggtgccccg cccgttccat gaagtctcgc cgcaggcatc
     4501 cagcgcaccg aaccagggcg gctcctggcc tggggtatcg gaccttggcc ctcgccaggc
     4561 cgccatgccc ccccggcgcg cattgacccg ccagttctag aaacacacgg gtgagcccca
     4621 gagttccacc cgcctggcag gcgcttaatt ctcttctacc cccttcaggc tcctcctaaa
     4681 caggcgggcg acccttcatc cggggtatcg aacgaagggc gcgggcgaaa gggcgcaggc
     4741 agccatccgg accttccctg tctcggaatc ccagggggag gagaaagccc gcaggcggga
     4801 gggggcgccg ctactcgcgc cctccctcgc tccaggctgc cagccgacgc atcgcgtcca
     4861 ccttgtcctt atcgcccagg cgcgcggatt ccaccgtctg ccgcaacacc tggatcgagt
     4921 ggtcgtaggt acgacggtcg actgggaagg gggtgccatc cttgccaccg tgagcgaagg
     4981 cgtagcgggc cgggtcgcgc agactcgacg gcacgccgtg gagcagctcc gcgatcagcg
     5041 cgagcgcgcg caccgccttg gggccgacgc cctggatctc caggatctcc tggaagcttc
     5101 ctggcgcgcg ctcataggtc ttggcaaaga tgcgctccag cgaatcgggg tgcaggtcgg
     5161 agacgagaag ttggtggtgc cggggcagcg taagctcctg catccggcgg atctcccggg
     5221 agagcttctc gggatgctcg ccggccagta cggcggcggt ttgccgggcg gcttccgcct
     5281 ccgcagcgac caggttgagc accagcgact cgcgccggga tgcgcacacc gccgcgtgtg
     5341 gctcgcagac gaaggggtga tctggcttcc ccagccagtg ataccgccgg gcccacttca
     5401 ggtcggtatt cattccctgc tagacaacgg cccaatctcc ctcgcgcgtg aagaagaagc
     5461 agtggtggta gatctggtag ccatcctgca ccgcggtgct atctaccttg gcggacatgc
     5521 ggctggcgta gaccagctct tcccccggca cggcgagagt gtgccgctcc gcgtgcgcgg
     5581 cgatctccag gggagtgcgc cgcgacacgg cgccttttcc gcccgctacg aagagcccca
     5641 gatccgcctc gcggcccttt actgcctctt tgagcgcacc gcaggcggtg gtggtcaccc
     5701 ctgaagagtg ccagtcgaaa ccgaggacgc atccgaacgc ctgaaaccag tagggatccg
     5761 agaggcggcg cagcacttct gcaggccccc actcggtgac aatgtactcg accatggcgc
     5821 gcgacaggcg caccatgcgc gcgaaaagcc acgggggcgc gtgcccgcca tgcaggggga
     5881 gccccgccgt tcctgttcgc attgtcttca gccttccagg aagtaaacct gccgccgatg
     5941 gatcacgccg cgagcgcgcg ggcgagcgcc aggggcgagg gcgcgacggc ctccacgggc
     6001 acgcccagcg cctccgccat ctgggagagc gtcatgtcgt ccaggaagcg cccggcgttc
     6061 aggagcacat ccggcacata gacgcgattg gccgggccag cggcgcggat cgccgcgagg
     6121 atatcgcctc cggtgaggag accggtcacg gtgatccgct cgccgaagaa gcggttggcg
     6181 acggcgagcg cctccacgcg gtgcccggtg atccgcgata gttccccggc gagcgcctcc
     6241 accatcggat gggccagcat cccggtcacc aggaacacgc gctcaggctg gggtacgggt
     6301 ttcgccggtc ggcctcgcag ctgctccagc tcgtccagga agagccgtga gagaccgatt
     6361 ccatcttctg tctgcggaaa ctcctcatac gcatcccgtg gcggaagcgg ttctccggcc
     6421 aggatgtaga actcgtcggc ggcgaagacc aggcgcgtac cgtgctccgc gagaaaccgc
     6481 tcgcgccacc gctccaggcg tgccagcgtc tcgcgcgctc cctccggggt gaccggctca
     6541 agagccgtca gtcgctcacg atagcgcgtg agtccgaccg gcacgaccgc gatggagagg
     6601 acctccgggt gcagcgcggc caggtctgcg agggtttgat cgaggacctc gccatcgttg
     6661 aagctggggc agaggacgat ctggcagtgc atttcgatcc cggcatcggc caggcggcgc
     6721 atgagcggga gcacctcggc gttgccggcg cagcggagga ggcgcgcgcg cgctcccgga
     6781 tccgtggcgt gcacggagat gaagagcggg ctcaggtgca gccgcaagat acgccggaga
     6841 tcctcctcgg tgacgttggt gagcgggacg tagttgccct gggtcaccga gagccgatag
     6901 tcgtcatccg gaatgtagag cgacttgcgc atccccctgg gcatctggtg aaggaagcag
     6961 aagacgcagg agttgcggca ccggcggatt ccgtcgaaga cgccctgggt gaaggtgatc
     7021 cccaggccct gatccacctc cttatggatc tgcacgctcc gcggctgccc gtcgcgcacc
     7081 agatccaggc gagcgaccgt gtcgctaatc aggaagcggt aatcgatcag atcggcaaac
     7141 gcctcgccat tgatcgccgt gatccgatcg cccgggcgca cgccggcgcg ttccgccagg
     7201 cttcccgggg caacgcactc gaccaccgcc ccatgctcat tccactccgt catactgcca
     7261 ttgtaacacg gcacgcggcg cggcgcaacc gcgggcgggc cggagccggc cgcgggcgtg
     7321 cctggcaaag gggtcgggcg aagccgaaac attcgcgaat gccaggtgtc taatctcttg
     7381 gcaagggcgg aatacggggt ggggcgccac gcccgaaccc agaagccgtc cgcctcagca
     7441 gcctggagtg acgcagcatg aaatgcaatg catcgctgga ggagctatct gcctacgccg
     7501 acggagaact cagccccgcg gaaatgtccg cgatcgaggc gcaccttggc gtgtgcgacc
     7561 ggtgccgcaa ggagttggcg tcactgtgtg gggtgagcca ggcgttccgg ctggcgggtg
     7621 atctcgagcc gggcctctcc gtgcgcagga gcgtcgcccg ccgtctggcg atgaccgagg
     7681 aagcgccgga gttcacctgc gcgcggatgc agatgagcat ctctgcgatg ctggatggcg
     7741 agctgtccga ggaggatgcg ctcatccttc aagcccacct ctccacctgc tcggcgtgcg
     7801 cttccgaggt cgcctcgctg gagtgggtgt ccgattcggt gcgcgcgctt cccgaggtcg
     7861 agctgccgtt tggcctggag cggcggattc gcaccgcgat tgcccgggag gagcaaggcg
     7921 ttttctccct gggctggatg cgacgcctct tccagccgcg gatggccccg ggcatggcga
     7981 cggcattggc gggagccacg ctcttggtgg gcttctggct cggtcgtctc tatccccagg
     8041 ctcccggtac tgcgccggtg gcggtggcgc cgcccgcgaa accacaggtc gccacccgca
     8101 tggtggctcc caacaccacg attcgcgtca ccgagcagcc tgcgcctttg cgcgcgaagc
     8161 cgcagcggcg tccggcggcg cccgttcagc gcgcgcccga gccgcggcgc gtcgccgtcc
     8221 ggacagtgcc cgcgccgaag cccgcgctcg cgcgccacga ggaggcgcgg cctgcccctg
     8281 cgcatcaaag tgcgcacagg ccggagagcg cgccttctcc cactgccgta gcgaccactt
     8341 caccacttcc tatcgtccgc ccgcttcccg aagcgccgcc gccggtcgtc gagttgccca
     8401 cgccaccggc acccgcccgg gtcagcgtcg agatcgccat ggcctggc
//
LOCUS       DAOSCV010000048         8440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5489_length_8440_cov_4.042695, whole genome shotgun sequence.
ACCESSION   DAOSCV010000048 DAOSCV010000000
VERSION     DAOSCV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5489_length_8440_cov_4.042695"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3574
                     /locus_tag="PLY56_02155"
     CDS             <1..3574
                     /locus_tag="PLY56_02155"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20312.1"
                     /translation="YERVLGAGVHHPDAPLQLSWMADQPVPTGTVEYGTGGRFDQRAE
                     SAEISLRNHRVLLKPLAPGRYQGRVRIPFGQTEWTSPPFEFTVPAPARPGRSRAYRVP
                     LTLLEPTAAGRKAWPVTSGIPLPKGVLASAADARIEDSRGRPVPSRCVVTSAWPDGSA
                     RWLVVTFLADTEAGKSTRYTLVCGGRQPGQAAAAPPIRVLQNTAEDGFALFRDLALDL
                     NGNGIAEPGEIFSGEPELGNLRLLDAAQRTFGMAPGAKVRSFRNALRHDVISEGHYAG
                     PEWEAPFRWRAEMTAFAGQPWMRLRFSVANPRIKEPHTLLSGAGLTLPIAGTGSVEGA
                     FEEFAFQRVGEGETPVLLQDLDDHFSIRGQGSPREGERALGFASVRRDGKCLTAFVRD
                     FWQTYPKALALKSDGLHVRLLPLLPPNAYEKESADGDALIRLFYPYRNGKYQFNRGLE
                     FMTELYLLLEQGAAPGQRQEMSRYAQWFNNPLYAVPDPMVACATGALGPVSPRVEGEF
                     DAYTHLVEKGFAAIEERRQEKREYGWLNYGDWHGERRFNWGNLEYDLQWALGLEFLRS
                     GSLKYLWRGAQAAQHSVTIDTVYEPWSSRMARLQWTHSVGHIGNFFDRNDDRFRKFGN
                     VFGLSRPDAPNPFVAGAIDVAGHTFVGGNFLYAMLLGDPRMLQVTERVATHQAAYLTP
                     SFDFSIERAAGWPLINAVEAYETTGNPFYLNAARLYVEKVLAKQDPEIGDFRLRHGPP
                     ECMHEPRHIGGKAFATGVLLYGLMRYHLLTDDPEVKRCILRSASWLARTSWNKETHAF
                     RYLSTCPTFGRRRGNGSTDLLCAPGMAYALTLKPDPEVREVLLDSLSRAFAAHVDNGK
                     GYAGMIRQTPYALHLLREKLGVRQIQPPAGSLGASVRPVLYVPLGESAPLHLIVTREA
                     SLPETCRVRVTSAPRGWKIEPRELAFRAPIGTSASPALQVRAEAGAKPGEVVLSCTMG
                     NRPAGDLRVRLMPRAPAVTGPAPDAAGLAVLGPSDTLTAQAFSSRPGVRVGIAPEEMT
                     RYRAVVLPCDFFASGSAKPEALLEQLSAFARGGGTVVLFQLNDDIWQPGFLPIDLMLS
                     DTNGELGSVDAPEHPLFAGVGNLDKVICYDTITYADPGWKVLARDTAGQPAILEAAIG
                     QGRILVIEPSLDRIAAGKETVPEGVRVEDCRRFIENLILWAIGG"
     gene            3763..4458
                     /locus_tag="PLY56_02160"
     CDS             3763..4458
                     /locus_tag="PLY56_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20313.1"
                     /translation="MRRMRRCESGFTLIELLVVIAIIAILAAILFPVFARARENARKS
                     NCQSNLRQIAMGLVQYVQDYDERMPHCRYTPDDFRAPSAYTGTPLTPSWPQFIAPYLK
                     NTGVLKCPSDDRARPHYTNGANNMELWSYGRNYGYFNGAKKNAADALALSEIAVPSDT
                     IMIGEGDNCNRIGPRSVDWPGNGNVSVDAKISTQLLEPRHMDGMNFAFFDGHVKWFRH
                     GGTPARLYSVEED"
     gene            4916..5611
                     /locus_tag="PLY56_02165"
     CDS             4916..5611
                     /locus_tag="PLY56_02165"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20314.1"
                     /translation="MRRMRRCESGFTLIELLVVIAIIAILAAILFPVFARARENARKS
                     NCQSNLRQIAMGLVQYVQDYDERMPHCRYTPDDFRAPSAYTGTPLTPSWPQFIAPYLK
                     NTGVLKCPSDDRARPHYTNGANNMELWSYGRNYGYFNGAKKNAADALALSEIAVPSDT
                     IMIGEGDNCNRIGPRSVDWPGNGNVSVDAKISTQLLEPRHMDGMNFAFFDGHVKWFRH
                     GGTPARLYSVEED"
     gene            complement(5691..6200)
                     /locus_tag="PLY56_02170"
     CDS             complement(5691..6200)
                     /locus_tag="PLY56_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20315.1"
                     /translation="MAAESEKPEFSVAPAAPEDWPWMLRRYERTAWESLTPDLRRHLS
                     PRDVRRQVSAQVEAFRSAAGDSFAALVARNRQGKRAGFIWVQENRHGFTGEARAYVLE
                     VYVSPTCRGKGLGKRLMDCAEEWARARGLERIALNVAAHNTPARTLYESLGYAVETLR
                     MAKELAGGD"
     gene            complement(6241..7494)
                     /gene="serS"
                     /locus_tag="PLY56_02175"
     CDS             complement(6241..7494)
                     /gene="serS"
                     /locus_tag="PLY56_02175"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ20316.1"
                     /translation="MLDPRWIRENTEEVRRNLERRRVTLNLDAWLQLDQRRTLTLQKV
                     EALRAERKAGSKGKPTEEQRERLRALGDQIARAEADLQAIEAEWRDLLEQIPNLTHPD
                     VPEGGEEDFRILQVVGEPTQFPFVPKDHVELGEAADLIDFRHAAEVAGSGFYYLKGGL
                     VALDLALQQYAFHQVLQEGFLPMVTPDLARQEVLKGIGFNPRGQEKQIYNIEESDLSL
                     IATAEITLGGYHMNEILAEDALPKAYAGLSHCFRTEAGSYGRESRGLYRVHQFSKVEM
                     FVFCRPEDSDAWHERLRDIEVKIFKGLGLPFRVIDTAAGDLGAPAYRKYDLEAWLPGR
                     GKYGEVTSTSNTTDYQARRLNIRFRRNETGKVEFVHTLNGTAVATSRAILAIMENYQQ
                     EDGSIRVPEVLVPYMGVEVIAGPGA"
     gene            7776..8438
                     /locus_tag="PLY56_02180"
     CDS             7776..8438
                     /locus_tag="PLY56_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20317.1"
                     /translation="MNRKQYGFTLIELLVVIAIIAILAAILFPVFARARENARKANCL
                     SNMKQLGLAVMQYAQDYDEVLPPTYNGGSTTYTLPNGNQHTGAVLWPTLLYPYVKNTG
                     VYVCPSDPKKWTGTYTGEMSYGMNTFQEEEALATITNPSETMFLAEAGGGDSYVLDGD
                     PGGTNEEMLGSAAYPMVPRHGDGLTSLFCDGHAKWMPAKSVPPYSLNSRYWNGAYTGT
                     NP"
BASE COUNT         1437 a         2769 c         2759 g         1475 t
ORIGIN      
        1 gtacgaacgc gtccttggcg cgggcgttca tcaccctgat gcacctctcc agctttcgtg
       61 gatggcggat cagccggtgc caaccggcac ggtggagtac ggcacgggtg gccgcttcga
      121 tcagcgcgcc gagtcggcgg agattagcct gcgcaaccac cgcgttcttc tgaagccgct
      181 ggcgccaggg cggtatcagg ggcgcgtgcg catccccttc gggcagacgg agtggacctc
      241 gccacccttc gaattcaccg tgccagcgcc ggcaaggccg ggtcgctcgc gcgcctaccg
      301 cgtgcctctc actctcctcg agcccactgc cgccgggcgc aaggcgtggc cggtgacctc
      361 cgggatcccg ctgcccaagg gagtactggc gagcgctgcg gacgcgcgca tcgaggatag
      421 ccggggtcgc ccggtgcctt ctcgctgcgt ggtgaccagc gcctggccgg atggcagcgc
      481 gcgctggctg gtggtcacct tcctggccga tacggaggcg gggaaaagca cgcgctatac
      541 cctcgtctgt ggcgggcgcc agcctggaca ggcggccgcc gcgcctccga tccgagtctt
      601 gcagaacacg gccgaggatg gtttcgcgct cttccgcgac ctcgccctcg acctgaatgg
      661 caacggcatc gcggagcccg gcgagatctt ctcgggagag ccggagctgg ggaacctgcg
      721 ccttctggat gctgcgcaga ggacgttcgg gatggcgccc ggcgcgaaag tgcgctcctt
      781 ccggaacgcg ctgcgccacg atgtgatcag cgagggccat tatgcgggcc ccgaatggga
      841 agcccccttc cggtggcgcg cggagatgac cgccttcgcc ggccagccct ggatgcgcct
      901 gcgcttctcc gtggccaacc ctcggatcaa ggagccgcat acgctcctca gcggcgctgg
      961 cctcaccttg cccatcgcgg gaaccggatc cgtcgaagga gcgttcgagg agttcgcctt
     1021 ccagcgcgtt ggcgagggtg agacacccgt attgcttcag gacctggatg accactttag
     1081 catccggggg cagggctccc cgcgtgaagg cgagcgtgcc ctcgggtttg ccagcgtgcg
     1141 ccgcgatggc aagtgcctca ccgccttcgt gcgcgacttc tggcagacct acccgaaagc
     1201 tctggcgctg aagtccgatg gcctgcacgt gcgcctgctt cccttgcttc caccgaacgc
     1261 ctacgagaag gagtcggcgg atggcgacgc gctcattcgc ctcttctatc cctaccggaa
     1321 tggcaaatac cagttcaacc gcggcctgga gttcatgacg gagctgtatc tcctgctgga
     1381 gcagggcgcc gcgccaggcc agcgtcagga gatgtcgcgc tacgcccagt ggttcaataa
     1441 ccccctctac gctgtgcccg acccgatggt agcctgcgcg accggcgcgt tgggtcctgt
     1501 gagcccgcga gtcgagggcg agtttgacgc ctacacccac ctggtcgaga agggcttcgc
     1561 ggcgattgag gagcggcgcc aggagaagcg cgagtacggg tggctgaact atggcgactg
     1621 gcacggcgag cgccgcttca actgggggaa cctggagtac gacctgcagt gggcgctcgg
     1681 cctagagttc ctgcgctccg gcagcctgaa gtacctgtgg cggggcgcgc aggccgcgca
     1741 gcactcggtg acgatcgata cggtttacga gccgtggtcg tcgcgaatgg ccaggctgca
     1801 atggacgcac tccgtcgggc acatcggcaa cttctttgac cggaacgacg accgcttccg
     1861 caagtttggg aatgtgttcg gcctgtcgcg ccccgatgcg cccaacccct tcgtggccgg
     1921 cgccatcgac gtggccgggc acacctttgt cggggggaac ttcctctatg ccatgctcct
     1981 aggcgacccg cgcatgctcc aggtgaccga gcgcgtcgcc actcaccagg cggcttacct
     2041 gacgccgagc ttcgacttct ccatcgagcg ggctgccggc tggccgctga tcaacgcagt
     2101 ggaggcctac gagaccaccg gcaatccctt ctacctcaac gcggcgcggc tctacgtaga
     2161 gaaggtgctc gccaagcagg atcccgagat cggcgatttc cgcctgcgcc acggcccgcc
     2221 cgaatgcatg cacgagccgc gccacatcgg cgggaaggcg ttcgcgaccg gcgtgctcct
     2281 ctacgggcta atgcgctacc acctgctgac cgatgacccc gaggtgaaga gatgcatcct
     2341 ccgctcggcc agctggttgg cgcggacctc ctggaacaag gagacgcacg ccttccggta
     2401 tctcagcacc tgccccacgt tcggcaggcg gcgcggcaac ggctcgaccg acctgctgtg
     2461 cgcccccggc atggcctatg cgctgacgtt gaagccggac cccgaagtgc gtgaggtcct
     2521 gctcgattcc ctctcgcgtg ccttcgccgc tcacgtggat aacgggaagg gctacgccgg
     2581 gatgatccgc cagacgccat atgccctcca cctcctgcgc gagaagctgg gggtacgcca
     2641 gatccagccg cccgccggat cgttgggagc gagtgttcgc ccggtgctct acgtgcccct
     2701 gggcgagagt gctcccctcc atctgatagt cacgcgggag gcctccctgc cggagacgtg
     2761 ccgggtgcgc gttacgagcg cgccccgggg atggaagatc gagccgcgag agctcgcctt
     2821 ccgcgcgcct attggcacat ccgcgtcgcc cgcgctccag gtgcgggccg aagcgggcgc
     2881 gaagccgggc gaagtggtcc tctcctgcac tatggggaat cggccagccg gtgacctgcg
     2941 ggtgcgcctg atgccgcggg cgcctgccgt caccggtccg gcgcccgatg cggccggcct
     3001 cgctgttctt ggtccatcgg acacgctcac cgcgcaggca ttttcctcca ggcccggggt
     3061 ccgggtaggg atcgccccgg aggagatgac ccgctaccgc gcggtggtgc ttccgtgcga
     3121 cttcttcgcc tccggctccg cgaagcccga ggcgctcctg gagcagcttt cggcatttgc
     3181 ccgcggcggg ggcaccgtgg tcctcttcca gctcaacgac gacatctggc agcccggctt
     3241 ccttccgatc gacctgatgc tgagcgacac gaatggggag ttggggagcg tggacgcgcc
     3301 ggaacacccc cttttcgccg gggtgggcaa cctggacaag gtgatctgct acgataccat
     3361 tacttacgcc gatcccgggt ggaaggtgct ggcgcgagat accgccggcc agcctgccat
     3421 cctcgaggcg gcgattggcc agggccggat cctggtgatc gagccgagcc tggaccgcat
     3481 cgccgccggg aaggagacgg tgccggaggg cgtgcgcgta gaggattgcc gccgcttcat
     3541 cgagaacctg atcttgtggg ccattggcgg ttgatccgtc agagcatagc gtgcaaaggc
     3601 cagcgagtgc acggccctga acggcgctta tcgaggttgc ggccagcccg atggcgcagg
     3661 ggaggcgccg cttgggatgc gcgccggact gggctgacgc tccggtgcgc ctgcgatgcc
     3721 gtaaaccggg ctgccacggc ggtcgcgttc aaggaggagc caatgagacg catgcggagg
     3781 tgtgagagcg gtttcacgct gattgagctg ctagtggtca tcgcgattat cgccattctg
     3841 gcggcgattc tcttccccgt tttcgcgagg gcgcgggaga acgcgcgcaa gtcgaactgc
     3901 cagagcaacc tgaggcagat cgcgatgggg ctggtgcagt acgtgcagga ctatgacgag
     3961 cggatgccgc attgccgcta caccccggat gacttccggg cgccgtcggc ctataccggc
     4021 acgcccctca ccccgtcctg gccccagttt atcgcgccct acttgaagaa cacgggcgtg
     4081 ctgaagtgcc caagcgatga tcgggcgcgc ccacactaca ccaacggcgc gaacaatatg
     4141 gagttgtggt cctacgggcg caactatggc tacttcaacg gggccaagaa gaacgccgcg
     4201 gatgccctgg cgctctcgga gatcgcggtg ccctcagata cgatcatgat cggcgagggt
     4261 gacaactgca accggatcgg cccgcgctct gtggattggc cgggcaacgg caatgtctcc
     4321 gtcgacgcga agatctccac tcagctcctc gaaccccggc atatggatgg catgaacttc
     4381 gccttcttcg acgggcacgt aaagtggttc cggcacggag ggacgccggc tcgtctctac
     4441 tccgtcgagg aagactgagc cattcgaggc tttcgccggt gaaggtccgc tctccccgcc
     4501 cggggggagc ggaccggtgt gcctggccaa ccctggcggg ctgttttcct gcgatggggt
     4561 gcggtgctat ccaccatccg tgaggtcggt cccagaggcg cattctggtg atcgagccga
     4621 gcctggaccg catcgccgcc gggaaggaga cggtgccgga gggcgtgcgc gtagaggatt
     4681 gccgccgctt catcgagaac ctgatcttgt gggccattgg cggttgatcc gtcagagcat
     4741 agcgtgcaaa ggccagcgag tgcacggccc tgaacggcgc ttatcgaggt tgcggccagc
     4801 ccgatggcgc aggggaggcg ccgcttggga tgcgcgccgg actgggctga cgctccggtg
     4861 cgcctgcgat gccgtaaacc gggctgccac ggcggtcgcg ttcaaggagg agccaatgag
     4921 acgcatgcgg aggtgtgaga gcggtttcac gctgattgag ctgctagtgg tcatcgcgat
     4981 tatcgccatt ctggcggcga ttctcttccc cgttttcgcg agggcgcggg agaacgcgcg
     5041 caagtcgaac tgccagagca acctgaggca gatcgcgatg gggctggtgc agtacgtgca
     5101 ggactatgac gagcggatgc cgcattgccg ctacaccccg gatgacttcc gggcgccgtc
     5161 ggcctatacc ggcacgcccc tcaccccgtc ctggccccag tttatcgcgc cctacttgaa
     5221 gaacacgggc gtgctgaagt gcccaagcga tgatcgggcg cgcccacact acaccaacgg
     5281 cgcgaacaat atggagttgt ggtcctacgg gcgcaactat ggctacttca acggggccaa
     5341 gaagaacgcc gcggatgccc tggcgctctc ggagatcgcg gtgccctcag atacgatcat
     5401 gatcggcgag ggtgacaact gcaaccggat cggcccgcgc tctgtggatt ggccgggcaa
     5461 cggcaatgtc tccgtcgacg cgaagatctc cactcagctc ctcgaacccc ggcatatgga
     5521 tggcatgaac ttcgccttct tcgacgggca cgtaaagtgg ttccggcatg gggggacgcc
     5581 agcccgtctc tactccgtcg aggaagactg agcggcctca ggattcagcc ggaacggccc
     5641 tggcgcccca acggtcgctg gggccgttcc cactcacggg gccggcgagg tcaatccccg
     5701 ccggcgagtt ccttggccat gcggagcgtc tctaccgcgt agccgagcga ttcgtagagg
     5761 gttcgtgcgg gagtgttgtg cgcggccacg ttcagagcga tccgctccag gccgcgcgcg
     5821 cgggcccact cctcggcgca gtccatcaac cgcttgccca gccccttgcc ccggcacgtg
     5881 ggcgagacgt acacctccag cacgtaggcg cgcgcctctc ccgtgaaacc gtggcggttc
     5941 tcctgcaccc agatgaagcc ggcgcgtttc ccctgccggt tgcgcgccac gagtgccgcg
     6001 aaggaatctc cagcagccga ccgaaacgcc tcgacctgcg cgctcacctg acggcgtacg
     6061 tcgcgcgggc tcaggtgccg ccgcagatcg ggcgtcagac tctcccatgc ggtgcgttcg
     6121 tagcggcgga gcatccaggg ccaatcctcc ggcgcggccg gcgcgacgga gaactccggc
     6181 ttttcactct ccgcagccat cgctgttgcc tccgtggcgc cgcgcgcctg cattgcgggc
     6241 ctatgccccc ggacccgcga taacctccac tcccatgtaa ggaaccaaca cctccggcac
     6301 gcgaatgctg ccatcctcct gctgataatt ttccataatg gctagaatcg cccggctggt
     6361 ggcaacggcg gtgccgttga gggtgtggac gaactcgacc ttgccggtct cgttgcgccg
     6421 gaagcgaatg ttgaggcgac gcgcctgata gtcagtggtg ttggatgtgg aagtgacctc
     6481 gccgtacttg ccgcggccag ggagccacgc ctccagatca tacttccgat aggcgggcgc
     6541 gcccaaatct cccgcggcgg tgtcgatgac tcggaagggg agacccaggc ccttgaagat
     6601 cttcacctcg atgtcgcgca ggcgctcatg ccaggcatcg gaatcctccg ggcggcagaa
     6661 gacgaacatc tccaccttgc tgaattggtg gacgcgatag aggccgcggc tctcgcgacc
     6721 gtaggagccg gcctcggtgc ggaagcaatg cgagagcccg gcgtaggcct ttggcagggc
     6781 atcctcagcc aggatctcgt tcatgtggta gccgcccagg gtgatctccg ccgtggcgat
     6841 cagcgagagg tcactctcct cgatgttgta aatctgcttc tcctgacccc tggggttgaa
     6901 gccaatgccc ttgaggacct cctgccgggc gagatccggc gtcaccatcg gaaggaagcc
     6961 ctcctgcagc acctgatgga aggcatactg ctgaagcgcc aggtcgagcg ccaccaaccc
     7021 gcccttgaga tagtaaaagc cagatccggc tacctccgcg gcatggcgaa agtcgatgag
     7081 gtcggccgct tcgcccagct ccacgtggtc cttggggacg aaggggaatt gggtcggctc
     7141 gcccaccacc tggaggatgc ggaagtcctc ctcgccgcct tctggcacgt ccgggtgcgt
     7201 cagattgggg atctgctcca acagatcgcg ccactcggcc tcaatcgcct ggaggtcggc
     7261 ttccgcccga gcgatctggt cgccgagagc acgcaaccgc tcgcgctgct cttcggtcgg
     7321 cttcccttta gagccagcct tccgttccgc ccgcagcgcc tccaccttct gcaacgtcag
     7381 cgtgcggcgc tggtcgagct gcagccacgc atccaggttc aacgtcacgc gacgcctctc
     7441 caggttgcgc cgcacctctt cggtattctc ccgtatccat ctcggatcca acatgctttg
     7501 ctatcctcca agcgcggctc ctggaagttg gcgggaaagc cgcgcaccaa cgcgaactca
     7561 tatggccgac cggtggatgt gcggacggcc cgtgtagaag agacttctct gcagcgcccg
     7621 catactcctc gctacaggaa ggcgatgagc acgcacatga aggaagcctc ccctgaagcg
     7681 cgaactctct ctgtgaggtc ttccgcaaag gaacgaaccg ggtttcaggc gtccgggcct
     7741 gccggtgttc catggggaga ggagaaatct tcggagtgaa cagaaagcag tacggtttta
     7801 cgctcattga gttgctggtc gtcatagcga taatcgctat acttgcggca attctgtttc
     7861 ccgtgttcgc gcgtgcgcga gagaacgcgc gcaaggcaaa ctgcctgtcc aacatgaaac
     7921 agctgggact ggcggtgatg cagtacgcgc aggactacga cgaagtgctc ccgccgacct
     7981 acaacggtgg cagcacgacc tacacgttgc ccaacgggaa tcagcatacg ggcgcggtgt
     8041 tgtggcctac gctgctctat ccttacgtga agaacacagg ggtatacgtc tgccccagtg
     8101 atcccaaaaa gtggacgggc acctacacgg gcgagatgtc ctacgggatg aacacctttc
     8161 aagaggaaga ggcgctggca acgatcacca acccgtccga gacgatgttc ctggcagagg
     8221 cgggtggcgg cgactcctac gtcctggatg gcgatcccgg tggtaccaat gaagagatgc
     8281 tcggctccgc ggcctatccc atggttccgc ggcacggcga cggcctgacg agcctcttct
     8341 gcgatggcca tgccaagtgg atgccggcca aaagcgtccc gccgtacagc ctaaacagcc
     8401 gctactggaa cggcgcctac accgggacca atccgtagct
//
LOCUS       DAOSCV010000049         8324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5582_length_8324_cov_4.044020, whole genome shotgun sequence.
ACCESSION   DAOSCV010000049 DAOSCV010000000
VERSION     DAOSCV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8324
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5582_length_8324_cov_4.044020"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..609
                     /locus_tag="PLY56_02185"
     CDS             199..609
                     /locus_tag="PLY56_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ20318.1"
                     /translation="MAGNTVVAGCGFHHVAIQTTDYEATKQFYTQVLGFRPANEWTMD
                     VRQLCLLDMGDGGYVEVVGIPESMVPEGNIKQFPMVHLAIRVADVDAAVDRVRAAGRP
                     ITIEPKDVLLGTVPARIAFFQGPNGEVIELFQEK"
     gene            611..1129
                     /locus_tag="PLY56_02190"
     CDS             611..1129
                     /locus_tag="PLY56_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOJ20319.1"
                     /translation="MEPQEPPSNRPTVSLEASAPGEAARMQPAASSLDPLERLGLIFF
                     AAALASLFFVVPEVWKILRSQASAVPFYKVTAAALATAYETDAQAAHERYGARQVSVT
                     GTVRRFGREANGVLYVELAASERRVVRCLFSEEDPGWLSGLLEGKEVTVHGTCEGRGP
                     SGDLTIRATDLK"
     gene            complement(1140..1793)
                     /locus_tag="PLY56_02195"
     CDS             complement(1140..1793)
                     /locus_tag="PLY56_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ20320.1"
                     /translation="MARERLDSTRSRPMTAEEAALMENNQRLAKWVANRVAKRVVARQ
                     GTDHNADTLFEDLLDAALFGLSLAAKTYRPEIGKFSTYAVTVMTGECNRFLERSGRKL
                     PPPDVHVISLSDLVPRDDNEGDAVGEFIADPNAIDPADVASRRALPGVLQDALATLPD
                     REAEVIRRRFLLGESLEKIGAALGRSRQRIYQIERQALSHLRSALQQRGYAAAADLI"
     gene            complement(2027..3205)
                     /locus_tag="PLY56_02200"
     CDS             complement(2027..3205)
                     /locus_tag="PLY56_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ20321.1"
                     /translation="METRDREIQDILNSLPTQSLNAINRLRQMGRPGVPALAVALKYP
                     DPTIRAGAARALARMNDPDGVPALAEAVADPDSSVRLAAILGLAEFPGAEAVQALAQA
                     LRARHALVRDCAARALACRSLSALRAALDDPDPVVRAAAAHGIGYYARDRRSASRLRE
                     MLDDEDGDVRIMAVRALAWISRRHPSVLPNLLIALEHPDENVRATGAYYAGRVGNRDA
                     CEALVRLLGDSSREVVLGAIRSLGSPDAEAHGTYRGVGDARAIPGLLAHLAGPDADLR
                     VAAAGALGCVLHADPYGERPPEMLTRLVERLEDSDARVRSAAATALGESSDGRAVEPL
                     VRALQDPVWSVRAAAASALGALGDARAIPALESATDDIDLAVARRAEAALHQLRVQLA
                     "
     gene            3876..4448
                     /locus_tag="PLY56_02205"
     CDS             3876..4448
                     /locus_tag="PLY56_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOJ20322.1"
                     /translation="MSYAGIALLMAIESACIPLPSELIMPFSGYLVSTGDFDLHSASI
                     AGAVGCAIGSAVAYWVGVWGGRPFLERYGRYVLIRRKDLDMADRFFERWGMWAVFISR
                     LLPVIRTFISLPAGISRMPFMPFLILSFAGSVPWCYLLTYVGKVLGENWQNIRHYFHG
                     ADLLIGAFLLVGFGFWIWHHFKPEPEQVKK"
     gene            4516..5358
                     /locus_tag="PLY56_02210"
     CDS             4516..5358
                     /locus_tag="PLY56_02210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20323.1"
                     /translation="MAEIRPMRAEEIPFGLELCRIAGWNQLVADWERLLALDPEGVFI
                     AEEQGTPCGTASAVRYGTRLSWIGMVLVHPDFRRRGIGSALMAHCIQYLQQHGVESIK
                     LDATDQGRPVYLKLGFRDERPCYRMTGARPDGLPSHPEVRPIAPGDWPKIAEMDRAAF
                     DADRLPLLRILHGEGPSAVIERGGELQAYGFARLGFNASYLGPLVSRNPDDARAVAET
                     LLAALPAGSVYWDILPDNAASLALVDSYGFQVIRQLTRMVLGASTGSDGVAQIYGAAS
                     FELG"
     gene            complement(5436..6245)
                     /locus_tag="PLY56_02215"
     CDS             complement(5436..6245)
                     /locus_tag="PLY56_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20324.1"
                     /translation="MSLDDWLQSGSWMTRPAGLGEAAGKLLEHLGGSYEKGRRAARAA
                     KQEGKRLLEDASEKCGVSEHVEAAKRRAEAARERAATAAETALRFIKERPAARIARAG
                     VQIKAGLTGFGAGYLFPDVEVLFLGASGRGRWLTQSGIPVWGAEKVVRSLLLEREPAG
                     GDDSQARFNVRRELYGAVAAPLLAGMSAGLGLRLTLDALSVRGVQGVGQLIVGGREML
                     FERIWMLGNGVWCFLMGKDLLLVTLGRSKNQTLSDVLQRLRDGDEDEEEPV"
     gene            complement(6482..7528)
                     /locus_tag="PLY56_02220"
     CDS             complement(6482..7528)
                     /locus_tag="PLY56_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20325.1"
                     /translation="MVNWGVIGCGGIADRRTIPGLLEAKNARLLAVMDQVPEVTKRVQ
                     EKYGCPRSYDSVADLLADREIDAVYIATPVFAHAEQVMAAAAAGKHILVEKPLAMNTG
                     QGREMIEACKKAGVYATEGYMMKFHSLHQRAKEIIDSGVLGKVVLMRAQLSCWYPEIP
                     GAWRQDPKLGGGGALIDMATHLYDLMRWFAGDVKEVSALADTLVFSYGVDDASSTLLR
                     FESGAHGFVDAFFCVPDSSVQSRLEIYGSRGSILAEGTIGQGSGGNMVAYIEENARGY
                     DAQQARDSADVKPQQVTAEPVNPYTAEVEYLSRCIEEKQAPQMNTLEEGLQILAIAEA
                     AYRAAKSGRREPIA"
     gene            complement(7726..>8324)
                     /locus_tag="PLY56_02225"
     CDS             complement(7726..>8324)
                     /locus_tag="PLY56_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOJ20326.1"
                     /translation="GLTLKNVDLYALSPEGKILWHERGEMLFTHFGVSGPLVLSASRH
                     LVGCTGCRLRIDLKPALTEEVLDARVQRDFHEAIRRQIGNALDKLLPRALIPVVLHVA
                     GLPEDLPVHQVTRKQRHALVRVLKGLMLTVTRPRPLAEAIVTAGGVSTHEIDARTMAS
                     RVVDGLYLTGEVIDVDGYTGGYNLQAAFTTGRIAGESV"
BASE COUNT         1427 a         2783 c         2649 g         1465 t
ORIGIN      
        1 accgtgcctt aacagaaaac cgggagaacc cgcatctaaa gacggacaag gcagtctggt
       61 tgaaggaagc ggagcaggaa atcgttaccc tccttatcat atcacccctt ccagaggatg
      121 tcaagcggaa ttggaaagga tctttgcgtc tgatcgccga atgagataca ccatcacaga
      181 gtaaggatga ggagcaatat ggcaggcaat accgtcgtcg cggggtgcgg cttccaccac
      241 gtggcgatac agacgaccga ttacgaggcg acaaagcagt tctacaccca ggtgcttggc
      301 ttccgtccgg ccaacgaatg gacgatggat gtgcgccagc tctgtcttct cgatatgggg
      361 gacgggggct acgtggaggt ggtgggcatc cccgaaagca tggtccccga gggcaatatc
      421 aagcaattcc cgatggtcca cctggccatt cgcgtcgccg atgtggatgc cgccgttgac
      481 cgcgtgcgcg cggccggccg ccccatcacc atcgagccga aagacgtcct cttgggcacc
      541 gtgcccgccc gcatcgcctt cttccaaggg ccgaatggag aagtgatcga gctgttccag
      601 gagaagtgat atggagcccc aggagccccc gagcaaccgg ccgacggtct cgctggaagc
      661 gagcgcccct ggggaagcag caaggatgca gcccgcagca tcatccctgg atccgctcga
      721 gcgcctggga ctcatcttct ttgccgctgc actcgccagc ctcttcttcg tggtgccgga
      781 agtgtggaag attctgcgct cgcaggcatc cgcggtgccg ttctacaagg tcaccgcggc
      841 ggcgcttgct accgcctacg agacagatgc gcaggctgcc catgagcggt atggcgccag
      901 gcaggtgagc gtgaccggca cggtgcgccg cttcgggcga gaggccaacg gagtgctcta
      961 cgtcgagctc gcggcctcgg agcggcgggt agtgcgttgc ctcttcagcg aggaagatcc
     1021 cggatggctc tccgggttac tggaaggaaa ggaagtgacg gtccacggca cgtgtgaggg
     1081 ccgtggaccg tccggtgacc tgactattcg agctacggat ctgaagtgac cgccgccact
     1141 cagatcaggt cggcggctgc ggcatagcca cgctgctgaa gcgcgctacg caggtgcgac
     1201 agcgcctggc gctcgatctg atagatccgc tggcggcttc gccccaaggc ggcgccgatc
     1261 ttctccaggc tctctcccaa caggaagcga cggcggatca cttcggcctc gcggtccggc
     1321 aacgttgcca gagcatcctg aagcaccccc ggaagcgcac gccgtgacgc gacatccgcc
     1381 ggatcgatgg cgttcggatc cgcgatgaac tcgccgaccg catccccttc gttgtcatct
     1441 cgcggcacga ggtcagagag gctgatcacg tgaacatccg gcggcgggag cttacgcccg
     1501 ctccgctcca ggaaccggtt gcactcgccg gtcatcaccg tcacggcgta agttgagaac
     1561 ttgccgatct ccggtcggta ggtcttggcc gccagggaga ggccgaacag cgccgcgtcg
     1621 agcaggtcct cgaagagcgt gtcggcgttg tgatcggtgc cctgccgggc caccactcgc
     1681 tttgccaccc gattggccac ccacttcgcc agccgttgat tgttctccat caacgccgct
     1741 tcctccgcgg tcatcggccg gctgcgggtg ctgtccagac gttctcgtgc cacctctcac
     1801 ctccatgcgc cactcatacg ctccatgtca cgcccagcct cgtgtcgcga gacctcgcgt
     1861 gaagccctca gtgggccgga tgggacctct ccgtgccgga ggatgcgccc cgctcaggcc
     1921 atgctcttca ggcatcgtca ctaccaggaa cacgcatgca aggggttcgg ttccagtgaa
     1981 ccatgcccca ggagagagag cgggtgaagg gagcgcggat tttagatcaa gcgagctgaa
     2041 ctcgaagctg atgcagcgcg gcctcggcac gccgggcaac ggccaggtcg atatcgtcgg
     2101 tggctgattc cagcgcgggg attgcgcgcg cgtcgcccag agccccgagg gcgctggcag
     2161 ccgccgcgcg caccgaccat accggatcct gtagcgcccg gacgaggggt tccacggcgc
     2221 gaccgtcgct actctcgccg agcgccgtag cagccgcgga gcgcacgcgg gcatcggagt
     2281 cctccaggcg ctctacaagg cgggtcagca tctccggagg acgctcgcca taggggtcgg
     2341 cgtggagaac gcagcccagg gcacctgccg cagcgacgcg aagatcggca tccggcccgg
     2401 cgagatgcgc cagcaaaccc gggatggcgc gcgcgtctcc aacgccgcga taggtgccat
     2461 gcgcctccgc gtctggggac cctagcgagc gaatggcgcc aaggactacc tcgcgactcg
     2521 aatcgccgag aaggcgcacg agggcctcgc acgcgtctcg attgccgacg cgcccggcat
     2581 agtatgcccc cgtggcgcgc acgttctcat ccgggtgctc caaggcgatg agaagattgg
     2641 ggagaacgct cggatggcgc cggctgatcc aggcgagcgc gcgcacagcc atgatgcgaa
     2701 catcaccgtc ctcgtcgtct agcatctcgc gaagacggga agctgaacgc cggtcacgcg
     2761 cataatagcc gatgccgtgg gccgcggcgg cgcgaacgac ggggtcaggg tcatccagag
     2821 cggcgcgcag cgcgctcagc gagcggcagg ccagggcgcg agccgcgcag tcgcgcacca
     2881 acgcatggcg cgcgcgcaac gcctgggcaa gcgcctgcac cgcttcggcg ccgggaaact
     2941 ccgcgaggcc caagattgca gccagacgaa cgctactatc cggatcagca acggcctcgg
     3001 caagagcagg aacgccgtcg gggtcgttca tccgggcgag ggcacgcgca gcacccgcac
     3061 ggatggtggg atcggggtac ttcagcgcca ctgccagggc cggtacaccg gggcggccca
     3121 tctgccgcag ccggttgatg gcgttcaaag attgggtcgg caggctgttg agaatatcct
     3181 ggatctcgcg gtcgcgggtt tccatctgcc tttcattata ccagatccac ctggcatgcg
     3241 aaagagggtc gttccccgga ttccgagata gggaatccct ttccgggcgc agaaaccggc
     3301 ggctccaatg ccgggcctct ccgggcccat tcagggccgg gagatggcca cgccgctggg
     3361 attccgacat atggagaagg ccgcacgcac gcgcggagct cgaagctccg cgctgcttct
     3421 ttgcaaagcc ctttcggggc tccccctccc agccaccgcc gttttgagac atctgggagt
     3481 atccccaacc cagcccggca gggcttcgct cgcaagcagc gcggtgattt atcgccgcgc
     3541 gccgggttgc gccttgcctc cgaggcttcg gattccttat gtcggaatct caggagactg
     3601 ccggctccgg tgttcatccg gccgttgggt gaaggttcgc cctgcaggcc cggattcctg
     3661 gcggaagcga tactcgccgt acttcatggg aaggagaagg ccccgtgcgc gcggaatctg
     3721 atggcaatag aagtatctcc cgcggctaat attgctctgg ccacgggagt tcttttgtgt
     3781 accgcctgcg gaggcgccgc gggcgatggg agggctcgca tttggaagca atcctggcac
     3841 caatcggcgc ttgggtggcc gatgtcattc gctccatgag ctatgctggt atcgcgctcc
     3901 tgatggccat tgagagcgcg tgtattcctc tgcccagcga gctgatcatg cccttctctg
     3961 gctacctggt ctcaacaggc gacttcgacc tccattccgc ttcgattgct ggcgcggtgg
     4021 gatgcgccat tggatcggcc gttgcctact gggtcggcgt ctggggaggc cgtcccttcc
     4081 tggagcggta tggtcgctac gtcttgatcc gacgtaaaga cctggatatg gcggatcgct
     4141 tcttcgagcg ttggggaatg tgggcggtct tcatcagccg cctgctgccg gtgatccgca
     4201 ccttcatctc gctgcccgcc gggatcagcc ggatgccgtt catgcccttc ttgatcctct
     4261 ccttcgccgg ctcggtgccc tggtgctacc tgttgaccta cgtgggaaag gtacttggcg
     4321 agaactggca gaacatccgt cactacttcc acggcgcgga cctgctcatc ggcgctttcc
     4381 tgctggtcgg gttcgggttc tggatctggc accacttcaa gccggagccg gaacaggtca
     4441 agaagtaagc tgcgttgcct gccaggctcg cggccagagt tcaaagccac gcgaatcgca
     4501 aggaggagta tgccgatggc cgagatccga ccgatgcgcg ccgaggagat cccattcggc
     4561 ctggagctgt gccggatcgc cgggtggaac cagctggtgg ctgactggga gcgcctgctc
     4621 gcgctcgacc ccgaaggcgt cttcattgcc gaggagcagg gaacgccgtg tggcacggct
     4681 agcgcggtcc gctacggcac gcgcttgtcc tggatcggga tggtgttggt ccaccccgat
     4741 ttccgccggc gggggatcgg atcggcgttg atggcgcact gcatccagta cctgcaacag
     4801 cacggcgtgg agagcatcaa gctggacgcc accgaccagg gccgccccgt ctacctaaag
     4861 cttggcttcc gcgacgagcg cccctgctat cggatgacag gcgcccggcc agatggactc
     4921 ccatcccacc ccgaggtgcg ccccattgcc cctggggatt ggccgaaaat cgcggagatg
     4981 gatcgcgccg cgtttgatgc ggaccggctc ccgctgctgc gcatcctgca cggcgagggg
     5041 ccgtcggccg tgattgagcg cggcggcgag cttcaggcct acggcttcgc gcgccttggg
     5101 ttcaacgctt cctacctggg gccgctcgtc tcgcgcaacc cggacgatgc gcgcgccgtg
     5161 gccgagacgc ttctggcggc tctcccggca gggagcgtct attgggacat cctgccggac
     5221 aacgccgcga gcctggcgct cgtggattcg tacggcttcc aggtcatccg acagctgacc
     5281 cgcatggtcc tgggcgcatc cacgggctca gacggcgtgg cccaaatcta cggcgcggcc
     5341 agtttcgagt tggggtgacg cgatagcgcg ccggcgcccc cttccaagga aggaaagact
     5401 ggcgagccca gaccgcgcgt cccccaagca ccggcctaca ccggctcttc ctcgtcttcg
     5461 tctccgtcgc ggagcctctg taggacgtcg gagagggttt ggttcttgga ccgcccgagg
     5521 gtgaccagga gcagatcctt gcccatcagg aagcaccaga ctccgttgcc cagcatccag
     5581 atccgctcga aaagcatctc ccggccgccc acgatcaact ggccgacgcc ctggacaccg
     5641 cgcacgctga gtgcatccag ggtcaggcgc aggcccaggc cggcgctcat gccggccagg
     5701 aggggagctg ccacggcgcc atacagttca cgccggacgt tgaacctggc ctggctgtca
     5761 tcgccgcccg cgggctcgcg ctccaggaga agggagcgca ccactttttc ggcgccccac
     5821 acggggattc cactctgcgt gagccaccgg cctcgcccac tggcaccgag aaagagtacc
     5881 tccacgtccg ggaagaggta gccggcaccg aatccggtga gccctgcttt gatctggacg
     5941 cctgctcgcg cgatccgcgc cgctggccgc tccttgataa agcgcaaggc ggtttcggcg
     6001 gcggttgccg cccgctcgcg cgctgcctcg gcccgccgct ttgccgcctc gacatgctcg
     6061 gagacgccgc acttctcgga ggcatcttcc aagagcctct tgccctcttg cttggccgcc
     6121 ctcgctgctc gccggccctt ctcgtaggat ccgcccagat gctccagcag cttgcccgct
     6181 gcttccccca gcccggccgg gcgtgtcatc caactcccgc tctgtagcca gtcatccaga
     6241 ctcatgcgat agccctcctg gttcctttac gcgggatcgt cacagccgcg cgcgagagat
     6301 gggcacggat actcgctctc cacccctgag gtcactatac ccgcttgatc atattcgtac
     6361 ctcgcgcgct cgcgacctat gatgagggcc caggacaaga cgccctgggc cccacgaggg
     6421 aatcccttcc ggattctcgg catcactctc aggcgacgcc ggaatagggt cactcgatcc
     6481 gttacgcgat gggctcgcgg cggccgctct tggcggcgcg gtaagccgct tcggcgatcg
     6541 cgaggatctg caggccctct tcgagcgtgt tcatctgcgg cgcctgcttc tcctcgatgc
     6601 accggctgag gtactccacc tcggcggtgt aggggttcac gggctccgcg gtgacctgtt
     6661 gcggcttcac atcggcggaa tcgcgcgcct gctgcgcgtc gtagccgcgg gcgttctctt
     6721 cgatgtaggc gaccatgttg ccgccggatc cctggccaat ggtcccctcg gccagaatgc
     6781 ttccccggct gccatagatc tccaggcggc tctgtaccga ggagtccggc acgcagaaga
     6841 aggcatcgac gaagccatgg gcgccgctct cgaagcgcag cagggtgctc gaggcgtcat
     6901 cgaccccgta gctgaagacg agcgtatccg ccaacgccga gacttccttc acatcgcctg
     6961 cgaaccagcg catcaggtca tagaggtgcg tggccatatc gatcaacgcg ccgccgccgc
     7021 ccagcttggg atcttgtcgc caggcgcccg ggatctccgg ataccagcag gagagctgcg
     7081 cgcgcatcag gacgaccttc ccaagcacgc ccgagtcgat gatctccttt gcccgctggt
     7141 gcaggctgtg gaacttcatc atgtagccct cagtcgcgta gacgcccgcc ttcttgcacg
     7201 cctcgatcat ctcgcgaccc tggcccgtgt tcatcgccag gggcttctcc accaggatgt
     7261 gcttgcctgc cgcggccgcc gccatcacct gctcggcatg cgcgaaaaca ggcgtggcga
     7321 tgtaaaccgc atcgatctcg cgatcggcaa gcaggtcggc cacggagtca tagctgcgcg
     7381 ggcagccata cttctcttgc acccgcttgg tcacttccgg cacctggtcc atgactgcca
     7441 gcaagcgcgc attcttcgcc tcaagcagac ccggtatcgt gcgacggtcg gctatccctc
     7501 cacaaccgat aacaccccaa tttaccatgg cagatcctcc ttcttgggca gggcaaacgc
     7561 gaagtggaaa cggaatggca gatcaaagac tgcaacttgc cagagcccgc ccgcgagaga
     7621 gggctccaac ggcccgcgcc acccttcggc ccctgggccg gcacggccct ctacttcaga
     7681 gcggttcggc gtctcccccg ccgcatcctt cctgatttca cggacctaca ccgattcgcc
     7741 ggcaatccgc ccggtcgtga aggcggcctg caggttgtag cccccggtgt agccatccac
     7801 atcgatcacc tcgccggtca ggtagaggcc gtccaccacg cgtgaggcca tcgtccgtgc
     7861 gtcgatctcg tgggtggaga caccgccagc ggtgacaatc gcctcggcca ggggccgggg
     7921 gcgcgttacg gtgagcatca gccccttgag aacgcgcacg agcgcgtgtc gctgcttgcg
     7981 cgtcacttga tggacgggca gatcctcggg caggcccgcc acatggagga caaccgggat
     8041 gagcgcccgc gggagcagct tgtcgagggc attgccgatc tgccggcgga tcgcttcgtg
     8101 gaagtcacgc tgcacgcgcg cgtccagcac ctcctccgtg agcgccggct tgagatcgat
     8161 gcgcaaccgg catccggtgc atccgaccag atgccgactc gcgctcaaga caagcgggcc
     8221 tgagacgcca aagtgcgtga aaagcatctc tccgcgctcg tgccagagaa tcttcccttc
     8281 tggcgagagg gcatagagat cgacgttttt cagggtgagc ccag
//
LOCUS       DAOSCV010000050         8314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5589_length_8314_cov_4.004359, whole genome shotgun sequence.
ACCESSION   DAOSCV010000050 DAOSCV010000000
VERSION     DAOSCV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_8314_cov_4.004359"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLY56_02230"
     CDS             complement(<1..388)
                     /locus_tag="PLY56_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HOJ20327.1"
                     /translation="MAFLLKKHLSPFLFPVPLCVLLLLAGVLLLWPAKNRRWGRALAS
                     AGLCVLLLGALPPFPEVGIRYLEAPWSVYKPVAGADVRWVVVLGGGVTVDPSLPPTTQ
                     LTRSSLVRLIEGIRILKLHPEARLLLS"
     gene            complement(405..1673)
                     /locus_tag="PLY56_02235"
     CDS             complement(405..1673)
                     /locus_tag="PLY56_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20328.1"
                     /translation="MYRLTPPAVYVSDRAMADARSAARVGRMLAALGCEERAIPFTDT
                     DIPEMIRAQGWETARRRQGTHAGHRDPALVFTTIRFDDRPDPKRLLEECPPGTPPSLV
                     HQLLGYGGRTVHRENPKHDRVCRCRYQFETIFGCPHGCCYCTGGQVSIIYVNLEELIE
                     RQIAPTLAGNPRQNVFMFNSALSDTLSFEPEYGLTQLMAELCAATEDRYYLIHTKSAN
                     VDFLRNIDHRGHTILLWSLTSPTVSRVVEPGSGTTEERIEAMGKCADAGYPVRVKFKP
                     IVPVRGWREEAEAMVDALLARVRPDNIGLCTIAWMPLAELRDCIDFSLMDPEFVCAME
                     DAEARMRGVHTGPIPPEMRARVYQFYLDAIRARDREVPVFLCTESPKLWQEFAPRLGM
                     SPGDYVCACGPQTTPGARRIAELWEPESVA"
     gene            1911..3905
                     /locus_tag="PLY56_02240"
     CDS             1911..3905
                     /locus_tag="PLY56_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ20329.1"
                     /translation="MSDELTAKSASGRVRPAFPKRAVITAGMPYGSKDLHFGHVGGVF
                     VFADMFARFLRDRIGSENVLFISGTDCYGSPIVEEHRKQTESGQFQGSLEEFVQYNHD
                     RQRKTLEDYQISLNLFAASGLGRAREIHAEFGAWILRTLYAHGHLERRTTPQWYDPER
                     GTFLTGRQVRGRCPVLGCKSENAYADECSLGHQYEPIDLIDPKSTLTGKRPEMRDVTN
                     WYLNIAGLRDVLLPWYEHLKESGEWRPWAVSSVLEWFEPPIIHVTRNQLEALEAVRPK
                     LPPHTLREGQAQSVQLVFERLEEMERARPILGENGIRYRVGKTLVPFRLTGNLEWGVP
                     APEIEGLEGLTFWVWPESLWAPISFTATCLEQQGQPASHWKEWWATRDATVYQFIGED
                     NVYFYGLAQMAIFLGMQEGRPTVDPPEGELRLTRIVANRHILFLDKKASSSGAVKPPL
                     ARELLDYYTVDQLRAHFLSLGLGERSVSFRPKPLNPKADPREADPVLKEANLLSNAFN
                     RAVRSCFYTAQKYFEGQLPEGTVSPEVVERTETAILDFEEAMAAHEFHRAIEITAELI
                     RENNQRWTKVNPYNEECDPEVRRQALIDSFHMVRAATVLMHPVAPQGTEKVREYLGVG
                     EEFWSWERIFEPLSAFLPAGHTFKFLQPKEDFFEKHPSQK"
     gene            4085..4897
                     /locus_tag="PLY56_02245"
     CDS             4085..4897
                     /locus_tag="PLY56_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ20330.1"
                     /translation="MEEQIRVRFHGNEGPTVIVLHGGPAAPGSAAPLARGLAGAFRVL
                     EPWQRGSGEEPLTVARHIADLHEVVLSCGFARPALVGESWGAMLALAYAAAHPESAGP
                     LTLVGCGTFDAAARAQIGATLEARTSPELRRLLERIPEEIPDPLAQMARRYELTHSLY
                     DYDPHPPEPDTESLPPFDLRAHTETWNDMIRLQEAGVYPATFAAIRSPVLMLHGAYDP
                     HPGQMIRNSLLPFLPHLEYREWKRCGHRPWAERHVRDDFFATLREWLSRHLR"
     gene            5118..5663
                     /locus_tag="PLY56_02250"
     CDS             5118..5663
                     /locus_tag="PLY56_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HOJ20331.1"
                     /translation="MGEELRVPAESLVVGNAEAVLAAAATVILVLAGVNVWLVARVSR
                     LSRSFRRLATGSNGENLEQLLHAHLDRVVAAVERADEVSNACQRLDERMTGCVQHVGM
                     LRFDAFDDVGGHLSFALALLDDAGNGIVLSSLHGRQETRMYAKPIRGGKPEVPLTVEE
                     AQAMRQAGFEAAPSRLVRSDR"
     gene            5772..7544
                     /locus_tag="PLY56_02255"
     CDS             5772..7544
                     /locus_tag="PLY56_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20332.1"
                     /translation="MENLRRFARYVFAYKWRVFGALGLILVLQGQGAVIPWLTKTLID
                     DVIPNRRMEMIKWVLVAILGLRLASGVISYWRTYLVSLVGQLVLFDLRNDIFRHLQKL
                     SLSYYEKNQAGKILARVMWDVQNVHQLFSSAFIQLISDTFAILIYVVILFSMSAKLAF
                     YSILVLPLYAWTFLILRPRIRRASQDLQEKFSYIVGSVSERITGAKVVKSFTRERSEQ
                     RRFVGDIREKTTLELDMIKLSTTLGTTSEFISGLGTAIILCYGSYLAIREEAGMTAGR
                     LVQFTSYLAQLYGPIVRVVQVNDVIQRASVALERIFEVLDTKPSVAEKPDAVPLPPVK
                     GHVVVENVSFGYDPDKLVLKGISLDVQPGQMIALVGPSGSGKSTLSNLIPRFYDPIEG
                     RILIDGYDLKDVTLHSLRSQIGIVLQETLLFSGTIRDNLRYGAPEATDEELVAAAKAA
                     NAHDFIIEQENGYDTEIGERGMKLSGGQKQRLAIARAILRDPRILILDEATSALDSES
                     EALIQEALERLMRGRTTFVVAHRLSTIMRADRIVVLDKGEIAEMGTHIELLENNGIYA
                     RLYIEQFKSQAEIFRDDKFRTLFD"
     gene            complement(7644..>8314)
                     /locus_tag="PLY56_02260"
     CDS             complement(7644..>8314)
                     /locus_tag="PLY56_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20333.1"
                     /translation="WEAVPIPNNFPWTNTDSSGQNPEATGKMREAFSRPVSPDKPAPD
                     VFVHMRIAAFAAGLGEIGYSKMFISPQFGPRQRLAAVLTDAPLDPDPLYSGPPLCDRC
                     MACARECSGGAISMEKTVKVTVAGRELEWGEIDYQKCSRYFCGASKAHNPFMTSKEDE
                     EGFNQPVGAAQRYKVGPTYDYGRALEGASGCIRACMVHLEERGKLQNTFHNRFRKRKP
                     WKLE"
BASE COUNT         1481 a         2698 c         2695 g         1440 t
ORIGIN      
        1 ccgagagtaa gagccgcgcc tccggatgga gcttcaggat ccggatccct tcgatcagcc
       61 ggaccagcga cgagcgagtg agctgcgtcg tcggtgggag cgacggatcc actgtgacac
      121 cgccgcccag cacgacgacc cagcgcacat cggcaccggc gaccggcttg tagacagacc
      181 agggtgcttc cagatagcgg atgcctacct ccgggaacgg cggcagcgcg cccagaagga
      241 gtacacatag gccggcgctc gccagcgctc gcccccatcg cctgttcttc gcgggccaga
      301 ggaggagaac gccggccagc aggagcagca cgcatagggg cacaggaaag aggaaaggtg
      361 agagatgctt cttgagaaga aaggccatcg ttgatgctcc aagcctacgc cacgctctct
      421 ggttcccaca gttcggcaat ccggcgcgcg cccggcgtcg tttgcgggcc acacgcacag
      481 acgtagtcgc ccgggctcat cccgagccgg ggcgcgaact cctgccacag cttaggggat
      541 tccgtgcaca ggaaaacggg cacctcacgg tcgcgcgccc ggatggcatc caagtagaac
      601 tggtatacgc gagcccgcat ctcgggcggg attggtcccg tatgcacgcc gcgcattcgc
      661 gcctcggcat cctccatggc gcacacaaac tccgggtcca tcaaactgaa atcgatgcag
      721 tcgcgcaact ccgccagcgg catccacgca atggtgcaga gcccgatgtt atccggacgc
      781 actcgcgcca ggagcgcatc caccatcgcc tcggcctctt cgcgccaccc gcgcaccggc
      841 acgatcggct tgaacttcac gcgaaccggg tagccggcgt cggcgcactt gcccatcgcc
      901 tcgatccgct cttccgtggt gcccgagccc ggctctacca cgcggctcac agtcgggctg
      961 gtcaggctcc acaggaggat ggtgtgcccg cggtggtcga tattgcgcag gaaatcgacg
     1021 ttggcgctct tggtatggat caggtagtag cggtcctccg tggcggcgca cagctccgcc
     1081 atgagttggg tcaacccata ctccggctca aaactgagcg tgtcgctgag ggccgagttg
     1141 aacatgaaga cgttctgcct cgggtttcct gccagcgttg gggcgatctg gcgctcgatc
     1201 agctcctcca ggttgacata gatgatagag acctggcctc cggtgcagta gcaacagcca
     1261 tgggggcacc cgaagatcgt ctcgaactga tagcggcagc ggcagacgcg gtcgtgcttc
     1321 gggttctctc gatgcacggt gcgcccgcca tacccgagga gctgatggac aaggctgggc
     1381 ggagttccgg gcggacactc ttcgaggagc cgctttgggt ccgggcggtc gtcgaagcgg
     1441 atggtggtga agacgagcgc cgggtcgcga tgcccggcgt gcgttccctg ccggcgccgg
     1501 gccgtctccc agccctgggc gcggatcatc tccggaatat ccgtgtcggt gaagggaatg
     1561 gcgcgctcct cgcaacccag cgcggccagc attcgaccca cgcgcgccgc cgaacgagca
     1621 tcggccatcg ctctgtccga gacatagacg gctggaggtg tcagacggta catagcttcc
     1681 tcctgagtgg catccaccgg attcattgcc agcacgtgcc cctccgggat tcgctgcagg
     1741 cggggcaatc ccttcgcgcc ccccgtctgg tgccccgcgg cggctcccct cccgcggggc
     1801 gagcgcgggg cctcgcttgc gggaaggtgg cgtcgttgct ataatgagag cagcaccgtc
     1861 cggcctgcac gaaccacaga ccgggcgata gaacggcaag gagagtacgc atgtcggatg
     1921 agttgaccgc gaagagcgct tcgggccggg tgagaccggc gttcccaaag cgggccgtga
     1981 ttacggccgg gatgccctat ggaagcaaag acctccactt cggacacgtc gggggtgtct
     2041 ttgtcttcgc cgacatgttc gctcgcttcc tgcgcgaccg catcggctct gagaacgtcc
     2101 tcttcatctc tggaaccgac tgctacggct cgcccatcgt cgaggagcat cgtaagcaga
     2161 ccgaatccgg gcagttccag ggaagcctgg aggagttcgt tcagtataac cacgaccgcc
     2221 agcgcaaaac gctggaggac taccagatca gcctgaacct cttcgcggcc tcgggtcttg
     2281 gacgcgcccg ggagatccat gcggagtttg gcgcgtggat tctgcgcacg ctctatgcgc
     2341 atggccatct ggagcgacgc accacgccgc agtggtacga cccggagcga ggcaccttct
     2401 tgacgggccg ccaggtgagg gggcgctgcc cggtgctggg ctgcaagtcg gagaatgcct
     2461 atgctgacga gtgctcgctg ggccaccagt acgagccgat cgatctgatt gacccgaaga
     2521 gcacgctcac gggcaagcgt cctgagatgc gcgatgtcac caactggtac ctcaacatcg
     2581 ccggcctgcg cgacgtgctc ctgccgtggt acgagcacct gaaggaatcc ggcgagtggc
     2641 ggccgtgggc ggtcagctcc gtgctagagt ggttcgagcc gccgatcatc catgtgacgc
     2701 gcaatcagct ggaagccctg gaggccgtgc gccccaagct gcctccgcac acgctgcgtg
     2761 agggccaggc acagtccgtg caactcgtct tcgagcggct ggaggagatg gagcgggcgc
     2821 gcccgatcct gggcgagaat ggcatccgct atcgcgtggg gaagacgctc gtacccttcc
     2881 ggctgacggg caacctggag tggggcgtgc ccgcgccgga gatcgagggg ctagagggcc
     2941 tcaccttctg ggtttggccc gagtcgctct gggcgccgat ctccttcacg gccacctgcc
     3001 tggagcagca gggccagccc gcatcccact ggaaagagtg gtgggccacc cgagacgcca
     3061 cggtctacca gttcatcggc gaggataacg tctatttcta cggcttggca caaatggcga
     3121 tcttcctcgg aatgcaggag ggccgcccga ccgtggatcc ccccgagggc gagctccggc
     3181 tgacgcgcat cgtcgcgaac cggcacatcc ttttcctgga taagaaggca agcagcagcg
     3241 gcgctgtcaa gccccccctg gccagggagc tgctcgacta ctacacggtg gatcaactcc
     3301 gggcacactt cctcagcctc ggcctcggcg agcggagcgt cagcttccgg ccaaagccgc
     3361 tcaacccgaa ggcggatccg cgcgaggcag acccggtgct gaaggaggcc aacctcctga
     3421 gcaacgcctt caaccgggcg gtgcgctcct gcttctacac cgcgcaaaag tacttcgagg
     3481 gccagcttcc cgaaggcacc gtgagccccg aagtcgtcga gcgcactgaa acagcgatcc
     3541 tcgacttcga ggaggcgatg gccgcgcacg agttccatcg cgcgattgag atcactgccg
     3601 agctgattcg cgagaacaac cagcgctgga cgaaggtcaa cccctacaat gaggagtgcg
     3661 accccgaggt gcgccgccag gcgctgatcg actccttcca catggtgcgt gcggccacgg
     3721 tgctgatgca ccctgtcgcc ccgcagggaa cggagaaggt gcgcgagtat ctcggcgtgg
     3781 gcgaggagtt ctggagctgg gagcggatct tcgagccgct ttccgcgttc ctgcctgccg
     3841 ggcacacctt caagttcctc cagccaaagg aggacttctt cgagaagcac ccgagccaga
     3901 agtgagccac cccgcttgct ggtagaagac gcgtgcttcc ggccaggtgc ctcgggcgtc
     3961 tggccgggcg acatggagcg gcgtgcgaca cgggagagaa gagcccgtga ggccggtgcc
     4021 ctgggcctcc agcgcatgga ggcccggtag gcaccggcct gctagatgga tgggagacgc
     4081 tacggtggaa gagcagattc gggtgcgctt ccacggcaac gaggggccaa cggtgatcgt
     4141 cctgcacggg ggccccgcgg cccccggcag cgcggcgccg ctcgcgcgcg gattggccgg
     4201 cgccttccgc gtgctcgaac cctggcagcg cggcagcggc gaggaaccgc tcaccgttgc
     4261 ccggcacatc gccgacctcc acgaagtagt cctctcctgc ggatttgccc gaccggcgct
     4321 ggtcggcgag tcgtggggcg caatgctggc cctcgcctat gcggcagccc acccggagag
     4381 cgccggcccc ctcaccctcg tggggtgcgg caccttcgat gcggcggcgc gcgcccagat
     4441 cggggccacg ctggaagcgc gcacatcccc ggagctacgc cgactcctgg agcgtatccc
     4501 ggaagagatc cccgatccgc tggcgcagat ggcaaggcgc tacgagctga cgcactcgct
     4561 gtatgactat gatccccacc ccccggaacc ggacacggag tcactcccgc ccttcgacct
     4621 gcgcgcgcac actgagacgt ggaacgatat gatccgcctg caggaagcgg gcgtctatcc
     4681 cgcgacgttc gcggcgattc gttcgccggt gttgatgctg cacggcgcct acgatcctca
     4741 cccggggcag atgatccgca acagcctgct tcccttcctg ccccacctgg agtatcgcga
     4801 gtggaagcgg tgcgggcatc gcccctgggc agagcgccac gtccgcgacg acttcttcgc
     4861 gactttgcgc gagtggctat cgcgccacct gcgctagaga gcagagcgag cccgatggag
     4921 ggagcgcctg tcaggcccgc agcggatcgt ttgccgcttc gccttccagc caggcggcat
     4981 cggccccgcc tgacgcgaac cctcattgat agcgccggca ggcaatcccc cgccaggaaa
     5041 gcacgagtgc aggtcgaagc ttaggtagtg ggcggatgca cgcagagcac gcacccggtt
     5101 acgcggcccg ataggagatg ggagaggagt tgagagtgcc ggccgagtcg ttggtggtag
     5161 ggaatgcgga ggccgtcttg gcggctgccg caacagtgat cctggtgctg gcgggcgtga
     5221 atgtctggct ggtggcgcgt gtcagccgcc tgtcacggag ctttcgccgt ctggcaaccg
     5281 ggtcgaatgg agagaacctg gagcaactgc tgcacgcgca cctcgatcgt gtggtggccg
     5341 cggtagagcg tgctgatgag gtgtcaaacg cctgtcagcg gctagacgag cgtatgacag
     5401 ggtgcgtgca gcatgtgggg atgctgcgct tcgacgcttt tgatgacgtc ggcggtcatc
     5461 tcagcttcgc gctcgctctc ctggatgacg cgggcaacgg cattgtgctg agctcacttc
     5521 acggacggca ggagacgcga atgtatgcca aaccgatccg tggcgggaaa cccgaggttc
     5581 cgctcaccgt cgaggaggcg caggccatgc ggcaggctgg gtttgaggcg gcaccctcac
     5641 gcctggtgcg atccgatcgg taaggggcag ggtcgcatga atgccccggg ttgccgccgg
     5701 gctcatcggg ggcgggcctg aggcacgagc ggggagggag agaaagctcc cggcggaggt
     5761 agtccgactc catggagaat ctgcggcgtt tcgcgcgcta tgttttcgcc tacaagtggc
     5821 gcgtcttcgg cgcgctgggg ctcatcctgg tgctccaagg ccagggcgcc gtcatcccct
     5881 ggctgacgaa gaccctcatc gacgacgtca tcccgaaccg ccgcatggag atgatcaagt
     5941 gggtgctggt ggccatcctg gggcttcgcc tcgccagcgg cgtcatctcc tactggcgca
     6001 cttacctcgt ctcgctggtc ggccagctgg tcctcttcga cctgcgcaac gacatcttcc
     6061 gccacctgca gaagctctcg ctctcatact acgaaaagaa ccaggcggga aagatcctgg
     6121 cgcgggtgat gtgggacgtt cagaacgttc accagctctt ctccagtgcc ttcatccagc
     6181 tgatcagcga caccttcgcc atcctcatct acgtcgtgat cctcttctcg atgagcgcca
     6241 agctggcgtt ctactcgatc ctggtcctcc cgctctacgc gtggacgttc ctcatcctgc
     6301 gcccccggat ccggcgcgcg agccaggacc tccaggagaa gttctcctac atcgtcggaa
     6361 gcgtctccga gcggatcacc ggcgccaaag tagtgaagtc cttcacccgc gagcgctccg
     6421 agcagcgacg tttcgtcggc gatatccgcg agaaaaccac gttagagctc gatatgatca
     6481 agctcagcac gacgctgggc accacctccg agttcatctc cggtctgggc accgcgatca
     6541 tcctctgcta tggcagttac ctggcaatcc gggaagaggc gggaatgacc gccggccgcc
     6601 tggttcagtt cacctcctat ctcgcccagt tgtacggacc gatcgtgcgc gtggtgcagg
     6661 tgaacgacgt catccaacgt gcgtctgttg ctctggagcg catcttcgag gtgctcgaca
     6721 cgaagccctc tgttgccgag aagccggatg ccgtgcccct gcctccggtg aaggggcatg
     6781 tcgtcgtgga gaatgtctcc ttcggctatg accccgataa gctcgtcctc aaggggatca
     6841 gcctggatgt gcagcctggc cagatgatcg ccctggtggg tccgagtggc tcgggtaaga
     6901 gcactctgag caacctcatc ccgcgcttct acgatcctat cgagggccgg atcctaatcg
     6961 acggctacga tttgaaggac gtgaccctcc actcgttgcg cagccagatc gggatcgtcc
     7021 ttcaggaaac gctcctcttc tccggaacga tccgcgacaa cctacgttat ggcgctccgg
     7081 aggccacgga cgaggagctg gttgccgcag cgaaggcggc gaatgcgcac gacttcatta
     7141 tcgagcaaga gaatggctac gatacggaga tcggcgagcg cggcatgaaa ctctcgggcg
     7201 gtcagaagca gcgcctggct atcgctcgcg ccatcctgcg cgacccacgc atcctcatcc
     7261 tcgacgaggc aacctccgca ctggattcgg aatcggaggc actgatccag gaagccctgg
     7321 agcgcctcat gcgcgggcgc accaccttcg tcgttgccca ccgcctctcc acgatcatgc
     7381 gcgctgaccg catcgtggta ttggataagg gcgagatcgc ggagatgggc acacacatcg
     7441 aactgctgga gaataacggc atctatgccc gcctctacat cgagcagttc aagtcgcaag
     7501 ccgagatctt ccgcgatgac aagttccgca ccctgttcga ctgatccgag gggctcctaa
     7561 agaccgcgtg cggagaggag cgctgtgtgc caacgctcct ctccgcacgc ccgcattcct
     7621 cttctctggg gtgccgtgtt cccctactcc agcttccacg gtttccgctt gcggaaacgg
     7681 ttgtggaagg tgttctgcag cttgccccgc tcctccaaat gcaccatgca ggcgcgaatg
     7741 cagccgctgg cgccttcgag ggcgcggcca taatcatagg tgggcccgac tttgtagcgc
     7801 tgggcagcgc ccacgggctg gttgaagccc tcctcatcct ctttggaggt catgaaggga
     7861 ttgtgcgcct tgctggcccc gcagaagtag cggctgcact tctggtaatc gatctcgccc
     7921 cactccagtt cacggccggc gacggtgacc ttcaccgtct tctccatact gatggctccg
     7981 ccgctacact cgcgcgcgca cgccatgcag cggtcgcaaa gcgggggccc ggagtagagc
     8041 gggtcgggat ccaggggcgc atccgtgaga acggcggcaa ggcgctgacg aggcccgaat
     8101 tgtggcgaaa tgaacatctt gctgtagccg atctcgccca ggccggccgc gaaggcagcg
     8161 atgcgcatat gcacaaagac atctggcgcc ggcttatcgg gcgagacggg ccggctgaac
     8221 gcctcgcgca tcttgccggt cgcctccggg ttctggccgc tgctatcggt gttggtccag
     8281 gggaagttgt tggggatcgg tacggcctcc caac
//
LOCUS       DAOSCV010000051         8314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5590_length_8314_cov_3.355007, whole genome shotgun sequence.
ACCESSION   DAOSCV010000051 DAOSCV010000000
VERSION     DAOSCV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5590_length_8314_cov_3.355007"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="PLY56_02265"
     CDS             <1..1780
                     /locus_tag="PLY56_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20334.1"
                     /translation="AEISGNVYLIGWTLLMPAVALLVALQVANSFLRRSKRIAPLSRA
                     ELLCIYLVVSASLPVAGFGMIRFLVLNLGNTFFFSTPENKWGEMQEHLPAWMVPNDPV
                     NAFAGFYRGYSTPPWGDWVVPIVVWSIYIALLVVAGLCFTLLIRRRWIEEERLTFPIA
                     ALPMEMTQPGGGFFRSPLLWAGFLLPFIGQSLLALNALFPSVPAFQIKAKWYPVFTSL
                     PWSAFGSIPIGFYPLSIGLAYLVPLDVSFSCWFFFLFTRLEAVFGAMAGYSQGATSSA
                     AARFPYAEEQSAGAWLMLGLLTLWGARAHLRRSWRSPAGIGLIVCSAGAVLFWVLAGM
                     QLWAAVVILGLYGLYLVTGMRIRAEAGGQWIFFPLAWNPNSLMVNTLGTAGLTPATLA
                     LTPIMHAFIIEARGHPMPFHLETWKMGEQVDLKHRQMIIAFLIGLAVALPVAFATSLI
                     EWYHSGAAVKAANYPLAKANIAYNDWRNWVNTPRTPDWAGIKGMVAGGGITVLLTFLR
                     GRYLWWPFHPIGYAAANTIIVRAFWLCFFIAWLSKLLLLRYGGARSYRQGLHFFLGIA
                     FGDIMTQAMWTIAGQLLGFEVYQFVS"
     gene            1841..2089
                     /gene="secG"
                     /locus_tag="PLY56_02270"
     CDS             1841..2089
                     /gene="secG"
                     /locus_tag="PLY56_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ20335.1"
                     /translation="MATVLFWIFSVLQFLVAIGLIAIVAMQTTKSEGLAGTLGGKSSI
                     SFQGRPGVEEQLQQWTTYLAVAFIVLSFIIAVFQMRIL"
     gene            complement(2202..2456)
                     /locus_tag="PLY56_02275"
     CDS             complement(2202..2456)
                     /locus_tag="PLY56_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ20336.1"
                     /translation="MRYGIPYRTLTPRGLQNLLVAGRCISTDRYLHGSVRVMPGCFIT
                     GQAAGIAAALAVERETDTRGFPIALLQDRLKAMGAHLPNA"
     gene            complement(2459..3541)
                     /locus_tag="PLY56_02280"
     CDS             complement(2459..3541)
                     /locus_tag="PLY56_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ20337.1"
                     /translation="MAYDELVFTRRIPVRHEVDIFVAGGGPAGIAAAIAAARQGRTVF
                     LAEGHTCFGGMGTAGLVPAFMQFGDGVHFLAAGIGREVYDRLWDLGGTGPDDRRDNPR
                     ASLSIDAEVLKRLYDEMVVEAGVRFTFQTQCIAVEMGAPGRVDHAICQAKSGLFAVKA
                     RVFVDCTGDGDLAVWAGAPYEKGDAEGNLMPGTLCSLWAEVDWDTVRASGLDPRQRIL
                     DAFKDKVFTYEDRHLPGMWRVGPRLAGGNIGHTFGVDSTDERSVTEALLWGRRQLLEY
                     ERFYKEYLKGFERMELVATGSLLGIRESRRILGDYVLNLEDFKKRAVFEDEIGRYSYP
                     VDIHASRPGEKEYAQFEEEFRTLRAA"
     gene            complement(3591..4442)
                     /gene="pyrF"
                     /locus_tag="PLY56_02285"
     CDS             complement(3591..4442)
                     /gene="pyrF"
                     /locus_tag="PLY56_02285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009971072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ20338.1"
                     /translation="MSFWESIRLRWSVANTMVCVGLDPDLERIRRILLAAGDPAADAS
                     AEDALYAFNRRIIDATADLAAVFKPQIAHYAACGEEGLRALKRTITYIHTRRPEVPVI
                     LDAKRGDIGSTAERYAQEVFEHFEADAVTVNPYLGGDSLEPFLAWEDRGVFVLCRTSN
                     PSARELQDLPVCTDGRPLYQEVARLAAEQWNSRGNLGLVMGATYPDDLRAVRAIAGDM
                     PFLVPGVGAQGGDVNAAVANGKDSAGTGLLVNSSRGILYASMGADFEAAARNAAQALR
                     DAINAAR"
     gene            complement(4439..5878)
                     /gene="tilS"
                     /locus_tag="PLY56_02290"
     CDS             complement(4439..5878)
                     /gene="tilS"
                     /locus_tag="PLY56_02290"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ20339.1"
                     /translation="MEVDPIQRFEQAVLGTIRQRGLLAPGERVVVGVSGGPDSLALLH
                     ALHALSGPLGIALHVAHLNHRLRRAAPEEARFVAETAERLGIPCTVEAVDVRAAQRQS
                     GLSLEHAARNERYRFFLRTAKAVNASKVATAHTADDTVETVLLNLLRGAGTDGLAGIP
                     PCRALAEGVVVVRPLIDAWRAEVEAYCRAMKLHPREDTSNRSRAFLRNRVRLDILPYL
                     TRHFGEEVKPNLRRLAELARPEVELLDHLTAEATTRSAKESEEGIAISVRALGELPLA
                     LRRRVARSALRRLAGDPTKIGYREVQRLLETLEGERADFDLPGPVHVRREGDQILLTP
                     PGVAETAPGWEVLLPVPGEAPLPDGSAITAEVGPRPPGFAVPRVPRAREVFLDAEAVT
                     PPLHARSRLPGDCFTPLGMTGRKSLQDLFTDAKVPVAARTRTPVVLDARGIVWVAGLA
                     LADRVKVTPETRRLLRLRWSEPLLEENGR"
     gene            complement(5927..6259)
                     /locus_tag="PLY56_02295"
     CDS             complement(5927..6259)
                     /locus_tag="PLY56_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ20340.1"
                     /translation="MFRSWPIFRAHPNAPARVIGVAVGTDTCETATAFQQATGFTFPT
                     GVDEQSEVRSSYQLKRVPSVILVDPAGFVLRKYIGNRDEFAPAIDTALDAIEKGISIP
                     SYDLEGAG"
     gene            complement(6798..7622)
                     /locus_tag="PLY56_02300"
     CDS             complement(6798..7622)
                     /locus_tag="PLY56_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ20341.1"
                     /translation="MTEFIICGSAAAEAWPAIFCNCRCCQEAFARGGKEIRTRTTYHL
                     GDTVHIDFGPDACTQMQRFRLPFDRLKHLLITHSHQDHWLPSELCYRRPGFSVVDEEN
                     ILTVHGNARVREKLEAAVGDDFPFYRLRFEEVRPGRPIDLGDGMRAVPVPANHDRKEM
                     CVFYLLQVGDRVLLQANDTGWFLDSAWEILRDYEIDIVVMDSTSGKIESREGHLGCRW
                     VVALRDEMLAQGILKTEHRFIANHFSHNGGWLHTELEEYFAPHGIEVGYDGMRIPV"
     gene            complement(7619..>8314)
                     /locus_tag="PLY56_02305"
     CDS             complement(7619..>8314)
                     /locus_tag="PLY56_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ20342.1"
                     /translation="QYDAAIAYADVCLQHLFTQLAALGALEETVVVITADHGEEMDEH
                     EMWFDHHGLYDTNLRVPLIMRCPDRLPRGKYVDGMVTLMDVAPTLLALAGLEEAAAKE
                     GMLGKNLLPLVRDEVAPGQGSTERIFLTENTWMRKRGVRTREWKLIRACEPDIHNRPP
                     IELYHLPTDPGEQYNLAQERPEVVAELSNALDEWIERRKRETGLPDPIETQGITLRSV
                     GKVATAVPEDQRL"
BASE COUNT         1320 a         2758 c         2743 g         1493 t
ORIGIN      
        1 cgccgagatc tctggcaacg tctacctgat cgggtggacg ctgctgatgc cagccgtggc
       61 gctcctggtg gcgctgcagg ttgccaacag cttcctgcgc cgctcgaagc ggatcgcgcc
      121 gctctcgcgc gccgagcttc tctgcatcta tctggttgtc agcgcctcgc ttcccgtggc
      181 gggcttcggg atgattcgct tcctggtgct caacctgggg aacaccttct tcttctcgac
      241 gccggagaac aagtggggcg agatgcagga gcaccttccc gcctggatgg tgccaaacga
      301 cccggtgaac gcctttgccg gcttctaccg cggttacagc acgccaccct ggggtgattg
      361 ggtggtgccg atcgtggtgt ggagcattta catcgctctc ctcgtggtag cgggtctctg
      421 cttcacgctc ctcatccggc ggcggtggat cgaggaggag cggctcacct ttcccatcgc
      481 ggcgctgccg atggagatga cacagccggg gggcggcttc tttcgcagcc cgcttctttg
      541 ggccgggttc ctgctcccct tcatcgggca gagcctcctg gccctcaacg cgcttttccc
      601 ctcggtgccc gcgttccaga tcaaagcgaa gtggtatccc gtcttcacat ccctcccctg
      661 gagcgcgttt gggagcattc cgatcggctt ctacccgctc tccatcgggc tggcctacct
      721 ggttccgctc gatgtctcct tcagttgctg gttcttcttc ctcttcacgc gcctagaggc
      781 ggtctttgga gcgatggccg gctattccca gggcgccacg agtagcgcgg cggcgcgttt
      841 cccctatgcc gaggagcagt cggccggcgc ctggctgatg ttgggcctgc tcacgctgtg
      901 gggagcgcgg gcgcacctgc gccgctcctg gcggtcgccg gccgggattg gcctgatcgt
      961 gtgctcggct ggcgcggtgc tcttctgggt tctggccggg atgcagctgt gggcggcggt
     1021 tgtcatcttg gggctctatg gcctctacct ggtcaccggc atgcgcatcc gcgccgaggc
     1081 gggcgggcag tggatcttct tcccgctcgc gtggaatccg aactcgctca tggtgaacac
     1141 gcttggcacg gccggcctga cgccggccac gctggcgctg acgccgatca tgcacgcctt
     1201 cattatcgaa gctcgcggcc atccaatgcc gttccacctg gagacgtgga agatgggcga
     1261 gcaggtggat ctgaagcacc ggcagatgat catcgcgttc ctgatcggcc tcgccgtcgc
     1321 cctgcccgtc gcgttcgcga cgtcgctgat cgagtggtac cactccgggg cggccgtgaa
     1381 ggcggccaac tatcccctgg cgaaggcgaa catcgcctat aacgactggc gcaactgggt
     1441 caacacgccg cgcacgcccg attgggccgg catcaagggg atggtcgcgg gcggggggat
     1501 cacggtgctc ctgacgttcc tgcgcgggcg ctatctctgg tggcctttcc acccaatcgg
     1561 ctatgcggca gccaacacca tcattgtgcg tgccttctgg ctctgtttct tcatcgcctg
     1621 gctctcgaaa ctgttgctcc tgcgctatgg aggcgcgcgt tcctatcgac aggggctcca
     1681 cttcttcctc gggatcgcct ttggcgatat catgacccag gccatgtgga cgatcgcggg
     1741 gcagctgctc ggcttcgaag tgtatcagtt cgtctcatga cagcgcttgc gttccgagtc
     1801 cgctttatgg taaaatgagc gcagcttgga ggaccgtgtc gtggcaaccg ttcttttctg
     1861 gatctttagc gtgcttcagt ttctggtggc gattggcctg atagcgattg tcgccatgca
     1921 gaccaccaag agtgaggggc ttgccggcac cctcggcggc aagagcagca tcagttttca
     1981 gggccgcccg ggcgtggaag agcagctcca gcagtggacg acctacctcg cggtcgcgtt
     2041 catcgtcctc agcttcatta tcgcggtgtt ccagatgagg attttgtgag cggccgttga
     2101 gctgaaggcc tttggggttg gatgcagttc ggcccctcag gccctccgga gtggggggct
     2161 tgaggggccg aactggtgta cgcccgatct ccaaacagga gtcaggcgtt cggcagatgg
     2221 gctcccatcg ctttcaggcg atcttgaagg agcgcgatgg ggaagccgcg cgtatccgtc
     2281 tcacgctcga ctgccagcgc ggcggcgatg ccggccgcct gcccggtgat aaagcatccc
     2341 ggcatcacgc gcaccgagcc gtggaggtaa cggtcggtgc tgatgcagcg gccggctacc
     2401 aggaggttct gtagcccgcg cggcgtgagc gtgcgatacg ggatcccgta tcgcacgctc
     2461 acgccgcgcg gagagtgcgg aactcctcct cgaactgcgc gtactccttc tcgccggggc
     2521 gcgaggcgtg gatatcaact ggataggagt agcgcccgat ctcgtcctcg aagaccgcgc
     2581 gcttcttgaa gtcctccaga ttgaggacgt agtcgcccag gatgcgccgg ctctcgcgga
     2641 tcccaagcag cgaaccggtg gcgacgagct ccatccgctc gaagcccttg aggtactcct
     2701 tatagaaccg ctcgtactcc aggagctgtc ttcgccccca caggagcgcc tcggtgaccg
     2761 agcgctcgtc cgtgctatcc accccgaagg tgtgcccgat gttgccgccc gccaggcgcg
     2821 ggccaacgcg ccacatcccc ggcaggtgcc ggtcctcata ggtgaagacc ttgtccttga
     2881 acgcgtccag gatccgctgg cgcgggtcga gaccggaggc gcgcaccgtg tcccagtcca
     2941 cttcggccca gaggctgcag agggtgccgg gcatcaggtt gccctccgcg tcgcccttct
     3001 cgtagggcgc cccggcccac acggccagat cgccatcacc cgtgcaatcc acgaacaccc
     3061 tggccttgac ggcgaagaga ccgctcttcg cctggcagat ggcgtgatcc acccgcccgg
     3121 gggctcccat ctcgacggcg atgcactgcg tctggaaggt gaagcgcacg cccgcttcca
     3181 cgaccatctc atcgtagagg cgcttcagca cctcagcgtc gatgctcagg ctggcacgcg
     3241 gattgtcgcg ccggtcgtcc ggcccggtgc cgcccaggtc ccagaggcgg tcatacacct
     3301 cgcggccgat gccagcggcc aggaaatgga cgccgtcgcc aaactgcatg aaagccggca
     3361 ccaggcccgc cgtgcccatc ccgccgaaac aggtatgccc ttcggccagg aagaccgtgc
     3421 gcccctggcg cgcggcggcg atcgccgcgg caatgccggc cggaccgccg cccgccacaa
     3481 agatatccac ttcatgtcgc accggaattc ggcgcgtgaa gaccaactcg tcgtatgcca
     3541 tgctttctcc tcatgcgcaa gcgctgatag actcgtaggc gagggtggga tcagcgcgcg
     3601 gcgttgatgg cgtcgcgcag cgcctgcgcg gcattgcgcg cggcagcctc gaaatcggcg
     3661 cccatcgagg cgtagagaat gccccgagaa gagttgacaa ggaggccggt gcccgcggaa
     3721 tccttgccgt ttgccaccgc cgcgttgaca tcgccgccct gcgcgcccac gcctggcacg
     3781 aggaacggca tatcgcccgc gatggcacgc acggcacgga ggtcatccgg gtaagtggcg
     3841 cccatcacca ggccgagatt gccgcgcgag ttccactgtt ccgccgccag ccgggccacc
     3901 tcctggtaga gcgggcggcc atcggtgcag accgggaggt cttgtagctc gcgcgcgctc
     3961 gggttggaag tgcggcagag gacaaagacg ccccgatcct cccatgccag aaacggctcc
     4021 agcgagtcgc cccccagata ggggttgacc gttaccgcgt cggcctcaaa atgctcgaaa
     4081 acctcctggg cataccgctc ggcggtgctg ccgatgtccc cccgcttggc atccaggatc
     4141 acgggcactt ccgggcgccg ggtgtgaatg taggtgatgg tgcgcttaag cgcgcgcagg
     4201 ccctcctcgc cgcaggcagc atagtgcgcg atctgcggct tgaaaaccgc agccagatcg
     4261 gctgtggcgt cgatgatgcg ccggttgaac gcatagaggg catcctccgc gctcgcatcg
     4321 gctgccgggt cgcccgcggc caggagaatc cgacggatgc gttccagatc cgggtctagg
     4381 ccgacgcaca ccatcgtgtt tgccacgctc cagcgcaggc gaatgctctc ccaaaaactc
     4441 atcttccatt ctcctctagt aacggttccg accagcgcag gcggagcagc cggcgcgtct
     4501 ccggggtgac cttcacgcgg tcggccagtg ccaggccagc cacccacacg atgccccgcg
     4561 catcgagcac gaccggcgtg cgcgtgcgcg ccgcgactgg caccttcgca tcggtgaaga
     4621 ggtcctgaag cgacttgcgt cctgtcatgc ccagcggggt gaagcagtcg cctggtaggc
     4681 ggctacgcgc gtgtaggggg ggcgtcacgg cctcggcatc caggaagacc tcgcgcgctc
     4741 ggggcacgcg aggcacggca aacccagggg gtcttggccc tacctcggcg gtgatggcgc
     4801 tcccatccgg gaggggtgcc tcgcccggaa ccggcagcaa gacttcccag cccggcgcag
     4861 tctccgccac gccggggggc gtcagaagga tctggtcccc ctcccgtcgc acgtgaaccg
     4921 gtcccggcag atcgaagtcg gcgcgctcgc cctccagagt ctccagcagg cgctgcacct
     4981 cgcggtaacc gatcttggtg ggatctccgg cgagccggcg cagggccgag cgtgcgaccc
     5041 ggcgccgcag agcgagcggc aactccccga gggcccgaac cgagatggcg atcccctctt
     5101 cactctcttt tgcgctcctg gtggtggctt ccgccgtcag gtgatccagg agttccacct
     5161 ccggacgggc cagctccgcg agccggcgca ggttgggctt cacctcctcg ccaaagtggc
     5221 gagtgagata ggggaggatg tccaggcgca cgcggttgcg caggaaggcg cggctgcgat
     5281 tgctggtgtc ctcgcggggg tgcagtttca tcgcccggca gtaggcctcc acctccgcgc
     5341 gccaggcgtc gatcagtggc cggacgacga ctactccctc agcgagtgcc cggcacggag
     5401 gaatcccggc caggccgtcg gtgccggcgc cgcggagcag gttgaggagc accgtctcta
     5461 cggtgtcgtc tgctgtgtgc gcggtggcca ccttgctcgc gttcaccgcc ttggccgtgc
     5521 gcaggaagaa gcgatagcgc tcgttgcgtg ccgcgtgctc caggctgagc ccggactggc
     5581 gctgcgccgc gcgtacatcg accgcttcaa cggtgcaggg gattcccaga cgttcggcgg
     5641 tctcggcgac gaagcgcgcc tcctctggag cggcccgcct cagccggtgg ttgagatggg
     5701 cgacgtgaag cgcgatgccc agggggccgc tcaaagcgtg gagcgcatgg aggagcgcga
     5761 gcgaatctgg cccgccggag acgccgacga ccacgcgctc gccaggggcc agcaggccgc
     5821 gctggcggat ggtgccgagc acggcctgtt caaaccgctg tatggggtca acctccacga
     5881 actctcctct ggtgatctcg gcctcacccg gaggccgcta tggccctcag ccagctccct
     5941 caagatcgta ggaggggatt gagatgccct tctcgatcgc atcgagcgcc gtgtcgatcg
     6001 ccggcgcgaa ctcgtcgcgg ttgccgatat acttgcgcag gacgaatccg gcgggatcca
     6061 ccaggatcac cgacggcacg cgcttcagct ggtagctgga tcgcacctcg ctctgctcgt
     6121 cgaccccggt ggggaaggtg aagccggtcg cctgttggaa cgccgtggct gtctcgcacg
     6181 tgtcggtgcc taccgcgaca ccgatcacgc gggccggggc gttggggtgg gcgcggaaaa
     6241 tcggccagct tcggaacaag cgaacggctg ttcgggcacg tcgacttcca gaagacgatg
     6301 aaggtgtgct caccggcgcc gcgggtaaac cgaacgatcc cgccatccgt ggccggcagg
     6361 cagaactccg gggcatgggc cccggcttcg atcgctgctg ccatcctcaa cctcctttgc
     6421 gcgcctgatg cgaacctcag gagtggtttg ccgcggggat gcacccccat tatagggcaa
     6481 ggagcgagga tctgtcaaaa gccagtcgcg gggctgcggg actgaaggaa gcacgacgag
     6541 cacagggatt ctgagataga tggagaggac catccacccg cgcggagcag gaagctccgc
     6601 gctgctttct tgcaaagccc ttcgggcttc ccttcccagc cctcgccgtt ctcaggcacc
     6661 cgggagtgtc gccaaaccca gcccggcagg gctccgttca caagcagcgc ggaggctaac
     6721 ctccgcgcgg ggcagaaccg ccgatgtgag cactgccggt gtcgcgtctg ccgcccgaat
     6781 ccgcggcgcc atcgcggtca caccgggatg cgcatgccgt cgtagcccac ctcgatgccg
     6841 tggggtgcga agtactcctc caactcggtg tggagccacc cgccgttgtg cgagaaatgg
     6901 ttggcgatga agcggtgctc cgtcttcaag atcccttgcg ccagcatctc atcacgcagg
     6961 gcgacgaccc agcggcaccc gaggtggcct tcgcgcgact cgatcttccc cgaggtggag
     7021 tccataacca cgatatcgat ctcgtaatcg cggaggatct cccaggcaga atcaaggaac
     7081 cagccggtgt cgttggcctg gaggagcacg cggtctccca cctgaaggag atagaagacg
     7141 cacatctcct tccggtcgtg attggcgggc accggcacgg cgcgcatgcc gtcgcccagg
     7201 tcgatcggcc ggccggggcg cacctcttca aaccgcaggc ggtagaaggg gaagtcgtcc
     7261 ccgacggccg cctccagctt ttcgcgcacg cgggcgttac catgcacggt caggatgttc
     7321 tcctcgtcga cgacagagaa gcccggccgg cggtagcaca gctcggaggg cagccagtga
     7381 tcctggtgcg agtgggtgat caggaggtgc ttcagccggt cgaacggaag gcggaaacgc
     7441 tgcatctggg tgcaggcatc agggccgaag tcgatatgca ccgtgtcacc gaggtgatag
     7501 gtggtgcggg tgcggatctc cttgcccccg cgcgcaaacg cctcctggca gcagcggcag
     7561 ttacaaaaga tcgccggcca cgcttcggcg gcggcactcc cacagatgat aaactcggtc
     7621 atagtctctg gtcctccggg acggcggtgg ccaccttccc cacggagcga agggtgatgc
     7681 cctgcgtttc gatcggatcg ggcagtccgg tctcgcgctt gcgccgctcg atccactcat
     7741 cgagcgcgtt tgagagctct gccacgacct ccgggcgctc ctgagcgagg ttgtactgct
     7801 cgccgggatc ggtagggagg tggtacagct cgatcggggg ccggttgtgg atatctggct
     7861 cgcacgcgcg gatcagcttc cattcgcggg tgcgcacacc gcgcttgcgc atccaggtgt
     7921 tctcggtgag gaagatcctt tctgtgctcc cctggccggg cgcgacttcg tctcgcacga
     7981 gcggcagcag gttctttccc agcatcccct ccttcgcggc tgcctcctcc aggccggcga
     8041 gggccagcag cgtcggcgcc acatccatca gcgtcaccat gccgtccacg tatttgccgc
     8101 gcggcaggcg gtccggacag cgcatgatga ggggcacgcg caggttggta tcgtagaggc
     8161 cgtgatggtc gaaccacatc tcgtgctcgt ccatctcctc gccgtggtcg gcggtgatca
     8221 cgaccaccgt ctcttcaagc gcgccgagcg ccgcgagctg cgtgaagaga tgctgcaggc
     8281 agacatcagc gtaggcgatc gcggcatcgt attg
//
LOCUS       DAOSCV010000052         8261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5629_length_8261_cov_4.236534, whole genome shotgun sequence.
ACCESSION   DAOSCV010000052 DAOSCV010000000
VERSION     DAOSCV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5629_length_8261_cov_4.236534"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLY56_02310"
     CDS             complement(<1..1089)
                     /locus_tag="PLY56_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ20343.1"
                     /translation="MGDKGQITLLIACRERPDLIGLDHVHTAQGVIRVVGQVHDSLEV
                     LQGVVGMRPDVLLIDGQAPEFDALNTTLEVSASTRSTGVIVLGGEDSADYLRRAMRAG
                     AKECLTASVDLDTLADAIAEVASIQRQLFPVDGPAASGDGSHLSRVITVCGGKEGVGK
                     TTIAVNLAVALARVSGDRVALLDLAFGDAAVMLNLTTHHGFAELQAQEGRVDPTSLRE
                     LMRQHEAGVSVLPRVARLRYLEQDPVDAYVVQDVVGYLREQFRYIVVDNPPMHLESEL
                     QSLSLADEVLCVTTPWDILTLRNTRAFLDAAVGTFCPSDRVRLVLNRSDDHAMVSRAD
                     VEKALQRTVSIYIPNDARLVASSINVGVP"
     gene            complement(1094..1975)
                     /gene="cpaB"
                     /locus_tag="PLY56_02315"
     CDS             complement(1094..1975)
                     /gene="cpaB"
                     /locus_tag="PLY56_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOJ20344.1"
                     /translation="MTKRQGLLLALVLACGSTLLTYLWYQGKAPAAEAARPGAEARKE
                     TSSAVAIQPASERTTAGGFAVPRHQRAVVITVDATQGAGALAQPSDHVDILLAAKDLQ
                     SHYLAETILQDVPVLGPTGTETNATAAPATRDGKRQIVVAVSPHDAQRLMAAQLRGDL
                     VITLRNPGDREYAYVRPVVNPAAPKPAPAPAPAAAAPAKPATPPAANPAPARRTAPRP
                     ARPAPRRPSMAVAGIPPEPVQVLPPPTRPVAPLAPVSRPAPAAPSASGSRSADSPAPK
                     AIEVVKGTSVQTVLVKE"
     gene            complement(2138..2689)
                     /locus_tag="PLY56_02320"
     CDS             complement(2138..2689)
                     /locus_tag="PLY56_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20345.1"
                     /translation="MTDVMTIKQLAEYLQLPVTSLYQMAQSGRLPAAKVGKHWRFQRQ
                     MIDEWLRHQSAWGSRRVLVVDDDDLVRQTFRDSLEVIGCQVCLAADGDECLALAERET
                     FDLIFLDLVMPGKDGVDVLSALRGRGCKTHVVLITAYPESDLLNEALKHGPITLIRKP
                     VGIAAVQSTAEMLLDLRRPAMHS"
     gene            3502..3927
                     /locus_tag="PLY56_02325"
     CDS             3502..3927
                     /locus_tag="PLY56_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOJ20346.1"
                     /translation="MADFVFVPHTADIAIRARGRDLPALLRAAALGVTAASLGRESLE
                     PHRVPGISPRRVVSEGAPDEEGLLVTFLNELIYLAETHAEVYTDVEILSHSCDAGVEA
                     LAYPDPDLSAIHSVKAATYYDLKIERDGEDLTATVVFDV"
     gene            4074..5120
                     /locus_tag="PLY56_02330"
     CDS             4074..5120
                     /locus_tag="PLY56_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20347.1"
                     /translation="MSRELALDTIYLRPTSRLAHTDYSLSYHTEYIREKTGLDPASQE
                     AHRRFYDFWGMDFLWGTNDGLHVNWGARGRVTDMGHAVYASDGSDRRAPARCPFETVE
                     EVWAFDAVAEYGLPDFDAQVQAYEQLVQNARRAWPDQLTAGGYYRTIVSGAIATFGWE
                     MLLLGAANVSRMEPVFDSFYRYTRHHMEAWAKTSVEVVIQHDDFVWAEGAFMHPDIYR
                     KVIIPRYAELWKPLRAAGKKVLFCSDGDYTEFAEEIVEAGADGLIFEPFVDFGWAAER
                     FGSSHCLVGSFTDCRDMAFGTWDQVRASMDRTFEVARKCKGVIFAVGNHLPPNIPKAM
                     MDRYIEYLTANWYR"
     gene            5132..5608
                     /locus_tag="PLY56_02335"
     CDS             5132..5608
                     /locus_tag="PLY56_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HOJ20348.1"
                     /translation="MIDPIEQAKQWTREGKSRFLNTFAFVPDDKLTWTPSPTALSALR
                     IAAHVGVTNQTLAGAIAGEALPTNDPAELSAMGDAAEKAITTREAAVRLIEESTEAVL
                     AAIDKVKPDEIDKIVETPFLKAPMSFFLTLFGSHMGQHAAQIDYLQTIWGDNEFHF"
     gene            5621..7954
                     /locus_tag="PLY56_02340"
     CDS             5621..7954
                     /locus_tag="PLY56_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20349.1"
                     /translation="MRLCYLAAFLCAVCALSSAQAAPKVGVLYSSWSNDAFKNEFDQH
                     LKTLGWPFEKLHNREVAQWIGRLDEFDLIVATSVANYDQPQDMAPYKDAWLRFLERGG
                     GILIIDANYNSVLDLWMSRLGPDFMLRTAGCAPYTKKHGGSPAIECDPTDPLLHVPHE
                     LPPLFEARGSIWAHVDAWGDGWRSLVTCADKKSLFATRDVGKGCVAVTSYFALRGDAN
                     QALAKGLLENLWTRVLGLRAGVAVTAFNAGAALPGPRQATLELRNTGKEAATYRAVLR
                     IGYDKEAPDQVAQLTATAAPGASARFSLPYTVTRRGSQRLAIDIMSGEKTLLTMQRTA
                     AVPPMLAMTIPDRHLYPPATTLPFSATIVPDAGVDLAKCAAELLLDGQVARRWEPAEA
                     AGSYIVDVSALPLGEHKATLRLVRGNTVLGTTEEVFYRHRPAKVRSREDGTLLVDEKP
                     FFPFGWYHVSWPFTAEQRLAFLRDIAAGGFNTVHAGIKQLDEWEPFLSEAEKLGVRVI
                     TEFGVDPIQVIMRYRDHPAVLAWNPGDEPDGSGVSPAEMLARYDRFKRADPDHPTYMV
                     LCVPSTYERYAGCAEIIAPDPYPIPKSPISLVTQSLTSARQEAAKHGRPIWGVLQCFG
                     YEKGPWSVPTFAECRNMTYQALLAGVKGIIYYTYADQGFDVSKHPQLWAEMKTLPPEI
                     ERLRPFLLDGTLRTLEVAQPDMVAGIWQLGERRILCVVSTSQKEPREVAIQLPTGAGT
                     TIKAFLPGQEPTLTLQAGKLTGKLAPLEVQVLELVSK"
     gene            8046..>8261
                     /locus_tag="PLY56_02345"
     CDS             8046..>8261
                     /locus_tag="PLY56_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20350.1"
                     /translation="MTGRERVACTLAGESIDRLPVLPILHTGFATILGVPLGDFFSSA
                     ETMARVMVEGCREFGFDGVQLSSGVTAE"
BASE COUNT         1472 a         2759 c         2672 g         1358 t
ORIGIN      
        1 cggcacgccc acattgatcg aggaggcgac caggcgcgca tcattgggaa tgtaaatgga
       61 gaccgtgcgt tgcagcgcct tctctacatc cgcccggctc accatggcgt ggtcgtcact
      121 gcggttcaac accaggcgca cgcgatccga cgggcaaaag gtgcccaccg ccgcatccag
      181 aaacgcgcgg gtgttgcgaa gggtcaggat atcccacggt gtggtgacgc acagcacctc
      241 atctgccagc gataggctct ggagctcgct ctccagatgc atcggcgggt tatcgacgac
      301 gatgtagcgg aactgctcgc gcaggtaacc aaccacgtcc tgaacgacgt aagcatccac
      361 cggatcctgc tcgagatagc gcagccgcgc cacgcgcggg agcacgctga ctcccgcctc
      421 atgctgccgc atcagctcgc gcaggctggt gggatccacc cggccttcct gcgcctgcag
      481 ctccgcgaag ccgtggtgcg tggtcaggtt aagcatcacc gcggcgtcgc caaaggccag
      541 gtcgagaagc gcgacgcggt ctccggagac gcgcgccaac gccacggcca ggttgacggc
      601 gatggtggtc ttgcccaccc cttccttgcc cccacagacg gtgatcacgc gggagaggtg
      661 gctgccatcg ccgctggcag ccggcccatc caccgggaag agctgcctct gaatggacgc
      721 cacctcggca atcgcatcgg cgagggtgtc gagatccacc gacgcagtga ggcattcctt
      781 cgcgccggcg cgcattgccc ggcgcaggta gtcagcagag tcctccccgc ctagcacaat
      841 gacgccggtg ctccgcgtgg acgcgctcac ctcgagcgtg gtgttgagag cgtcgaactc
      901 gggcgcctgc ccgtcgatca ggagcacatc ggggcgcatc cccacgactc cctggagaac
      961 ctccagggag tcgtgcacct ggcccaccac ccgaatgact ccctgggcgg tatgcacgtg
     1021 gtccagcccg atcaggtcag ggcgctcccg gcaggcgatg agtagggtga tctggccttt
     1081 gtctcccacg gccctactcc ttcaccagaa ccgtctgtac cgaggtgccc ttgaccacct
     1141 caatcgcttt cggcgcgggg gaatccgcgg agcgcgagcc cgaggcgcta ggcgctgccg
     1201 gcgccggccg cgagacagga gccagcggcg ctaccgggcg cgtgggcgga ggaagcacct
     1261 ggaccggctc gggagggatg ccagccaccg ccatcgaggg gcggcgaggc gccggacgtg
     1321 ccggacgcgg tgccgtgcgc cgcgcaggcg ccgggtttgc ggccggcggc gtagccggtt
     1381 tcgcgggggc tgctgccgcg ggcgcagggg caggcgccgg cttgggcgct gccgggttga
     1441 ccaccgggcg cacgtacgcg tactcgcggt ctccgggatt gcggagcgtg atgacgaggt
     1501 caccccgcag ctgcgcggcc atgagccgct gggcatcgtg cggactcaca gcgacgacga
     1561 tctgccgctt gccgtcgcgc gtggctgggg cagcggtagc gttcgtctcg gtaccggtcg
     1621 gcccgagaac gggcacgtcc tggagaatcg tctcggctag atagtgcgac tggaggtcct
     1681 tggccgccaa gaggatatca acgtggtctg acggctgagc cagcgccccg gcgccttggg
     1741 tcgcatcgac ggtaatcacg accgcgcgct gatggcgcgg cacggcgaaa cccccggcgg
     1801 tggtacgctc tgaggccggc tggatcgcaa ccgccgagga agtctctttg cgcgcctccg
     1861 cccctggccg ggccgcttcg gcggcgggcg ccttgccctg ataccacagg taggtgagga
     1921 gagttgagcc gcacgccagc accagcgcca gcagcaggcc ctgccttttc gtcatcgaac
     1981 cacacttcct atccccacgg actcggcttg ccgagccctc accccacgat gcgagaagga
     2041 ccctacgggc cttccactgc gccggattct ggcccgcccc cggcagggcg tcttgaggga
     2101 aactctggcc cccggcgtac ctcccggggg cattcctcta agagtgcatc gctggccggc
     2161 gcagatcgag cagcatctcc gcggtcgatt gcaccgcggc aatgccgacc ggcttgcgaa
     2221 tcagcgtgat cggtccgtgc ttgagcgcct cgttcaagag gtcgctctct ggatacgcag
     2281 tgatcagcac cacgtgcgtc ttgcaccccc gcccgcggag ggcgctgagc acatccacac
     2341 catctttgcc cggcatcacc agatcgagga agatgagatc gaacgtttcg cgctcggcga
     2401 gcgccaggca ctcatcgcca tcggccgcca gacacacctg gcacccaatc acctccaggc
     2461 tgtctctgaa agtttgtcgg accaggtcgt catcatccac caccaacacc cggcggcttc
     2521 cccaagcgct ctgatggcgc agccactcat cgatcatctg ccgctggaat cgccaatgct
     2581 ttcccacctt cgcggctggc agcctcccgc tctgcgccat ctggtagagc gatgtcaccg
     2641 ggagctgcag gtactccgcg agctgcttga tcgtcatcac atccgtcatc tggcccaccc
     2701 gtggtattga tggttattgg tggttactgc gtctctattt tcgattatgg cggatgccta
     2761 acggatctat agggtagcct ggcaggttga acaaccgcag gagggggtga tccacgacca
     2821 gacgtgcggc aaccacgaga ggcagccgca ggagtgggcg ctcgtgcgag gggagatgtg
     2881 agcgcgggat cggttatcgg cgtcagctca ggcctcagga ggagacaacg gcccgagccg
     2941 cccgtgacga aaagcaaacg aagcaacgcg gacggccacc gcctgccctt ctcccggggc
     3001 gggaggagaa gcggtgcgac agcgccgctt taggaaggga aacctgggaa tcagcgggtg
     3061 cgggaaaggc gcgccgggcg accagcgcga acgcgcacag gggccatgcg ggggcgcagc
     3121 gtcatgaccg tgatcagcag gcttgatgct accatcgaca gaagaaatac tcccatgcga
     3181 tcctcctcaa gacaatgggc gcaagcaaaa gagccaggcc agcaatcgct tccgaccact
     3241 cacccgacca atctcatctt accacgccga ttctttggtg tcaactggca ggcaagcggc
     3301 gttctcccag gccaacaggc acgagcctgg agacacaagc agcgcgggta ctgacgcccg
     3361 cgcgacccgt gcagtgcgga tgtctgtgcc cgcgcacgcc tgcagtatgg gcgtttgcgc
     3421 gatggaacag ggcttgccga gcccgcccca cgccgggtat aatgggttcc agcagaagca
     3481 ggcgaaaggg gcacctgagt gatggccgat ttcgtattcg ttccgcacac agccgatatt
     3541 gcgattcgcg cgcgcggccg cgacctgccc gccctcctcc gggcggccgc tctcggtgtc
     3601 accgccgctt ctcttggccg cgagagcctg gagccgcacc gtgttcccgg catctcccca
     3661 cgccgtgtcg tttccgaggg cgctcccgat gaggaggggc tgttggtcac cttcctcaat
     3721 gagctcatct acctggcaga gacccacgcc gaggtgtaca ctgacgttga gatcctcagc
     3781 cactcctgcg acgccggcgt ggaggccctc gcctatcccg acccggacct ctccgcgatc
     3841 cactcggtga aggccgcgac ctactatgac ctgaaaatcg agcgcgatgg cgaggatctg
     3901 accgccaccg tcgttttcga cgtatgacca tacccgcgcg tgcctcgaga gcggggccgg
     3961 catgtcatcg ccggggggaa tcgtcacatg acgggccgaa ccactatgcc agagtgctgg
     4021 cggcgggccc gcccacgcaa cggtgtccga acatgcttcg gccggaggtg ctcgtgagca
     4081 gagagttggc gctcgatacc atctatctca ggccgacatc acgcctcgcc cacaccgact
     4141 acagcctgag ctaccacaca gagtacattc gtgagaaaac gggcctggat cccgcgtcgc
     4201 aggaggcgca ccggcgcttc tatgacttct ggggaatgga cttcctatgg ggcacgaacg
     4261 atggccttca cgtgaactgg ggggcgcgtg gtcgcgtgac agacatgggg catgcggtgt
     4321 acgcctccga tgggagcgac cggcgcgcgc cagcgcgctg tcccttcgag acggtggagg
     4381 aggtgtgggc ctttgatgcc gtcgccgagt acgggctgcc ggatttcgat gcccaggtgc
     4441 aggcttacga gcagctcgtc cagaacgccc ggcgggcgtg gccggaccaa ctcaccgcgg
     4501 gcggctacta caggacgatc gtctcgggcg ccatcgctac cttcggctgg gagatgctgc
     4561 tgctcggtgc ggccaacgtg tccaggatgg agcccgtttt cgacagcttc tatcgctaca
     4621 cgcgccacca catggaggcc tgggccaaga caagcgtcga ggtggtgatc cagcacgacg
     4681 atttcgtctg ggcggaaggc gccttcatgc acccggatat ctaccgcaag gtgatcatcc
     4741 cgcgctacgc ggagctctgg aaaccgctgc gcgccgccgg caagaaggtc ctcttctgct
     4801 cggatggcga ctacaccgag ttcgcggagg agatcgtcga ggccggcgcc gacggcctca
     4861 tcttcgagcc gttcgtcgat ttcggctggg cggcagagcg ctttggcagc tcgcactgcc
     4921 tggtcgggag tttcaccgat tgccgcgata tggccttcgg cacatgggat caggtgcgcg
     4981 cgtcgatgga ccgtaccttc gaggtggccc gcaaatgcaa gggcgtgatc tttgccgtcg
     5041 gcaaccacct cccgcccaac attccgaagg cgatgatgga ccgctacatc gagtatctca
     5101 cagccaactg gtaccgctaa aggagagaac aatgatcgac ccaatcgagc aagcaaagca
     5161 gtggacccgt gaaggaaaga gccgcttcct gaacaccttc gcttttgtgc cggatgacaa
     5221 gctgacgtgg acgccctcgc cgacagccct tagcgcactg cgcatcgcgg cgcacgtagg
     5281 cgtcaccaac cagacgctcg ccggggcgat cgcgggagag gccctgccca ccaacgaccc
     5341 cgccgagctc tcggcgatgg gggatgccgc cgagaaggcg atcaccaccc gcgaggctgc
     5401 ggtccggctc atcgaagaga gcaccgaggc cgttcttgcc gcgatcgata aggtgaagcc
     5461 ggacgagatc gacaagattg tggagacgcc tttcctcaag gcgccgatga gctttttcct
     5521 gacgctgttc ggctcgcaca tgggccagca cgccgcgcag attgactacc tgcaaacgat
     5581 ctggggagac aacgagttcc acttctagag ggaggaaccg atgaggctct gctatctggc
     5641 cgccttcctc tgcgctgtgt gcgctctctc gtctgcccag gcagcgccga aggtcggcgt
     5701 tctctacagc tcctggagca acgacgcgtt caagaacgag ttcgatcagc acctcaagac
     5761 gctagggtgg ccgttcgaga agctgcataa ccgggaggtg gcccaatgga tcggtcgcct
     5821 cgacgagttt gacctgatcg tggccacgag cgtggccaac tacgaccagc cgcaggatat
     5881 ggcgccgtat aaggatgcct ggctgcgctt cctggagcgc ggcggcggaa tcctgatcat
     5941 cgatgccaac tacaattcag tgctcgacct ctggatgagc cgcctggggc ccgacttcat
     6001 gctgcgcacc gcgggctgcg cgccctacac caagaagcat ggcggctcgc ccgccatcga
     6061 gtgtgatccg accgatcccc tgctccatgt gccgcacgag ctcccgccac tcttcgaggc
     6121 ccgaggcagc atctgggcac acgtcgatgc ctggggggat gggtggcgtt ccctggtcac
     6181 ctgcgcggat aagaagagcc tcttcgccac acgcgatgtg ggcaaggggt gcgtcgccgt
     6241 caccagctac ttcgcgctcc gcggtgatgc caaccaggcg ctcgccaagg gcctgctgga
     6301 gaacctctgg acgcgcgtcc tcggattgcg tgccggagtc gcggtgaccg ccttcaacgc
     6361 gggcgccgcg ctgcccggtc cccgacaggc caccctggaa ctgcgcaaca cgggcaaaga
     6421 ggcggcgacg taccgcgccg tgctccgcat cggctacgac aaggaggcgc ccgaccaggt
     6481 ggcgcaactg actgcgacgg ccgctcctgg tgcctccgcc cgcttctcgc tcccctacac
     6541 ggtcacccgc cgcggaagcc agcgcctcgc catcgacatc atgagtggcg agaagacgct
     6601 gctcaccatg cagcgcacgg ctgcggtgcc cccgatgctc gcgatgacga tcccggaccg
     6661 gcacctctat ccgccggcca ccacgctccc cttctccgcc actatcgtac cggacgcggg
     6721 cgttgacctc gccaagtgcg ccgcagagct gctcctggat ggtcaggtgg cccggcggtg
     6781 ggaaccggcc gaggcagccg gcagctacat cgtcgatgtc tccgcgcttc cgctcggaga
     6841 gcacaaagcc acgctgcgcc tggtgcgcgg caacacggtc ttgggcacca cggaggaggt
     6901 gttctaccgc caccggccgg caaaggtgcg ctcgcgcgag gatggcacgc tgctggtcga
     6961 tgagaagccg ttcttcccct tcgggtggta ccacgtctcg tggccgttca cggcggagca
     7021 gcgcctcgcc ttcctgcgcg atatcgccgc gggcggattc aacacggtcc atgcgggaat
     7081 caagcagctg gacgagtggg agccgtttct cagcgaagcg gagaagctcg gcgtgcgcgt
     7141 catcaccgag tttggggtgg atccgatcca ggtgatcatg cgctaccgcg accacccggc
     7201 ggtgctggcc tggaacccgg gcgacgagcc ggacgggagc ggtgtctcgc ccgcggagat
     7261 gctcgcccgg tacgaccggt tcaagcgtgc ggatcccgac cacccgactt acatggtgct
     7321 gtgcgtgcct tcgacgtacg agcgctatgc ggggtgcgcg gagatcatcg cgccggatcc
     7381 gtatcctatt ccgaaatcgc ccatctctct ggtaacccag agcctcacct cggcgcgcca
     7441 ggaggccgca aagcatggcc ggcccatctg gggcgtcctg cagtgctttg gctacgagaa
     7501 gggcccctgg agcgtgccca ccttcgcgga atgccgcaac atgacctacc aggcgctcct
     7561 cgccggggtc aaggggatta tctactacac ttacgccgat cagggcttcg atgtctcgaa
     7621 acacccgcag ctctgggcag agatgaaaac cctgcccccg gagatcgagc gcctgcgccc
     7681 gttcctccta gacggcacgt tgcgcacgct ggaagtggcg cagccggata tggtggccgg
     7741 catctggcag ctcggcgagc gccgcatcct ctgtgtcgtg agcacctcac agaaggagcc
     7801 gcgtgaggtc gccatccaac tgccgacagg cgcaggaacg accatcaagg cgttcctgcc
     7861 cggccaagag ccaaccctga ccctgcaagc agggaaactc accgggaagc tggcgccgct
     7921 cgaggtgcag gtactggagc tggtcagcaa ataacccatc ctccggcacg cttcgctgga
     7981 gttacgagcg cccgccagag aaatgccggc gggcgctctt cacacgaccc gagaaagaag
     8041 aacccatgac aggcagagag cgcgtggcct gcaccctcgc gggcgagagc atcgaccggc
     8101 tcccggtgct ccccatcctg cacacggggt ttgccacgat acttggcgtg cccctcggcg
     8161 acttcttcag ctccgccgag acgatggccc gggtgatggt cgagggttgt cgggagttcg
     8221 gattcgatgg ggtgcagctc tcctccggcg tgaccgccga g
//
LOCUS       DAOSCV010000053         8247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5642_length_8247_cov_3.389526, whole genome shotgun sequence.
ACCESSION   DAOSCV010000053 DAOSCV010000000
VERSION     DAOSCV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5642_length_8247_cov_3.389526"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLY56_02350"
     CDS             <1..1110
                     /locus_tag="PLY56_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20351.1"
                     /translation="VELARTLTALIRQGVLPPPRRTIRFMHAVEVHGFLPFIDANRDR
                     LGRQVVAGLCLDSVGLDLAHDGGFLVLYRSPEQNASFVDGLMETLLGAVSAEPAARFS
                     RDNYATFPWRTAPFWGNDAFISDGFFDIPTPQLTTWPDRYYHSSHDTPDRMSADTLGR
                     VGAAAGSFLYLLARADAAEARWFGLLAADDWKRRICRAVTEKITGGLSEPNPAAADQL
                     VASVRHLGLQARDAVMQAARFAQGDPGANEVLRALADDVSAFAEREAIGGARLLDPTV
                     EVARPAASAGEPDGEIVARRLRWHAPSPQTLSPASQERLAALGPNAARIWPWINGRRT
                     AREIEERLRFEGQIPLETVVEYLRLMSAEGAVEIL"
     gene            1156..3360
                     /locus_tag="PLY56_02355"
     CDS             1156..3360
                     /locus_tag="PLY56_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ20352.1"
                     /translation="MGTQATVRPGAQGEAARRRIEEAYRALREKDVRCTTLERVRLLI
                     EADAAVQGLPQPLQLGHGLTYLLERIGLPLSPDDQLLGRIHEEVPDPEGERLFQEAVT
                     RWGGDSRPAWLRDSGHECFAWERLLRLGLSGLEAFARSERERRKAEGAKEASLHFLKG
                     AELVYAGLRAYARRYAAAARAAGLEEAGARCAAIAERPPRHFAEALQLIWLVGHVYCT
                     MVAANPTLTFGRLDGLLLPFYREDLAAGRLTPDEAGDLIEEFYCKNNLILGRGEHQMG
                     GDPEKDTGWRRNLCYDSPQYVVLGGHRRDGSPVANDLTRLFLERVVPGFKNPVIVLRW
                     TDDLPEPLWRLACEKMRANASMMVYNDAAVIPALVDAGIAEEDAVTYTMHGCNWPDVP
                     GIQRTVSHCEPPLPKLFLTALLGGEGETPPVLPCIDTLYARFFALFRQEVAKKCDEIR
                     AHRARWDELAPGTLRVDDCFLDGPVARARSWQLGGVQYHTITCTLHSLATTADCFAVV
                     DELVFRARRVSLEELREALRTDFAECEPLRRLCQEAPKFGRDDDCADRHAVRLIEGLL
                     AAIDEASGRGTPEAVTLFRCLETDMRHRLTGAQTGATPDGRHAGMPTSENTSPSPGSC
                     TRGLTAMLHSLAKLPLHRICSGALNIRLQPRLVAGEEGLSRLAALLRAYFRMGGLQAQ
                     LSLADTATLRDAQEQPEAHRDLVVRITGYSALFTDMTRGAQEEIIRREEHAA"
     gene            complement(3368..5557)
                     /locus_tag="PLY56_02360"
     CDS             complement(3368..5557)
                     /locus_tag="PLY56_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ20353.1"
                     /translation="METTGRILLLLGMALLCVPALGAEYFLSPDGNDENPGTRARPWR
                     SIARANETLRPGDTATFLPGRYAGVIEPGVSGEKGKPITYRSEQRLGAILTGGKSSDG
                     LSLCVRLKERDHITIEGFHLLPEAGDWVLFETTRYCILRRCRMEGASRSYIPARMIDC
                     HYNRYEELEVWRSVQLGQTGHVSGNMFQLNASSHNVIEKCHFGRAGHCPFCLWIDSHH
                     NVVRGCVFDGRWGRNFELFTAPRTLFEGCVITNTYHGSGSADGRAKLFTRDGIFRRNL
                     VYRNYYQPLSIHAYRYQQMEPFGMISSRLYQNTFVANYESGFEMFDISARPDPHMVRG
                     NVLLNNLFAFNDADGDGVALNLGPSIGRDNRFIHNLFCGPKPGTPSIRYAWNDGRPGA
                     LRTAAEANAACPDQFTGNLDGDPRFVDAERDDYRLRPDSPALDAGAPLTVTREAGRGR
                     TLPVEDARPFYDGFGIPGEQGDLIFIGPRKRQARVVKVDLDANTLLLDREVSWEKGEG
                     VSLPYVGKAPDLGAYERGAENEAWYRAPRVPEGVRLPTMETATEPTVVVDFEPENREE
                     WFYRWYLNRQPNTTSRIDNTTAASGKHSLRVFATDDGAVMGALIRPRGWDIDRFPLVR
                     FSYRVPPGVPVGIWVEGFPQAMRGGGMVCLGGSPARACGRSRDLKKVLLVDDGEWHEV
                     VVDVRAIREVHPEVRRLRTFYLYTQGNGKKGQEYWLDHFRITPEGKE"
     gene            complement(5570..6472)
                     /locus_tag="PLY56_02365"
     CDS             complement(5570..6472)
                     /locus_tag="PLY56_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ20354.1"
                     /translation="MIARKGIWMMLLSAVFFAAMSALVRGATGVHSYTMVLARFVVGT
                     VLVALFFVFGLEKMRWRNWPWIVARGVSGGIACILSYWAIQEVGLARAVTLHHTYVIF
                     AALFAVPLLGERVGLRQWVAIFTAAVGTGLLCGVGMQAVSYGDLVALVSGIAAGFAIV
                     CVTRCRRTDSASNIFWSQSIFGIVLVAWPAARVWSPPTPAEWLVLLAIGLLATAGQLT
                     MTYAYKFTGAAYGSLLSLLSPVLTAAIGILYFGEHQGAGFYTGAFLVLLACADLSLHP
                     VRRSAQDVADALMEETASAKPVLR"
     gene            complement(6747..7859)
                     /locus_tag="PLY56_02370"
     CDS             complement(6747..7859)
                     /locus_tag="PLY56_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20355.1"
                     /translation="MMPIIPLPDAVPVPAPIWLVHFLLLLTFTLHLVPMSFTLGGGIL
                     AAVAAFRGRRDENQRRLSEVIARALPSVTAFTITTGVAPLLFVQVVYGQLFYTSSVLM
                     AWLWMGVIPLLIAGYYGYYACALAPRALGKHQPWVATASAVAAAAIGFLWVMNVLLML
                     SPEKWSGIYAQSAAGLYVNVTDPVVWPRFAHMLVGALAVTGVWIAVLSRAQAPVAPEF
                     ATWMRRQGIGLFTGATAVNLAVGTWFLFSLPKPALDLFSGGMTPSLLLLGLGILLGVA
                     AIGLLRVHLWLGTAAIAVALACMAILRHQVRLSYVAPYFNPETLAVQPQWGVFAIFAV
                     ALVVALGIVGWMTVTYLKAGRKKEAGSAREASTTAS"
     gene            complement(7856..>8247)
                     /locus_tag="PLY56_02375"
     CDS             complement(7856..>8247)
                     /locus_tag="PLY56_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOJ20356.1"
                     /translation="AVTRTGVLKSALWSSEKEVTPHNRLAAGEEVFRLQCRSCHTLRG
                     YNGVLPLMAGWDEAYTDYQLSRLSTLKPYMPPFMGTPEERAALAAWLARLAGPPSPPP
                     DALGVDGRPAANALAPAAELPVTEVKR"
BASE COUNT         1381 a         2864 c         2756 g         1246 t
ORIGIN      
        1 gtggaactgg cgcgaacact cacggcgctg atccgccagg gcgtgcttcc gcccccccgg
       61 cgcacaatcc gcttcatgca cgccgtggag gtgcacggct tcctcccctt catcgacgcc
      121 aaccgcgatc gcctgggtcg ccaagtggtt gccgggctct gcctcgattc ggtgggcctg
      181 gatctggcgc acgacggcgg cttcctggtc ctctatcgct cgcccgaaca gaacgcatcc
      241 tttgtcgacg ggctgatgga gacgctgctc ggtgccgtgt ccgcagagcc ggcggcgcgc
      301 ttctcgcgcg acaactatgc caccttcccc tggcgcacgg cgcccttctg gggcaacgat
      361 gccttcatct cggatgggtt tttcgatatc ccaacgccgc agctcactac ctggcccgac
      421 cgctactatc acagctcgca cgacacgccc gaccgaatga gcgccgacac cctggggcgc
      481 gtcggcgccg cagccggatc gttcctctac ctccttgccc gcgccgacgc cgcggaagcc
      541 cgctggttcg ggctgctcgc cgcggacgac tggaagcgtc gcatctgccg ggccgtcacc
      601 gaaaagatca ccggggggct gagcgaaccg aaccccgcgg cggccgacca gctcgtggcc
      661 tccgtgcgtc atctcggcct gcaggcacgc gatgccgtga tgcaggcggc acgcttcgct
      721 caaggcgatc ccggcgcgaa tgaggtgctg cgcgcccttg cggatgatgt atcggccttt
      781 gcggagcgcg aagccatcgg cggggcacgc ctgctggatc ccacggtgga ggtggcccgg
      841 ccagcagctt cggctgggga gcccgatggt gagatcgtgg cccgccggct gcgctggcac
      901 gcgccatccc cgcagacact ctcccccgca agccaggagc gcctggccgc gctcggaccg
      961 aatgccgcgc gcatctggcc ctggatcaac gggaggcgga cggcacgtga gattgaggag
     1021 cgtctgcgct tcgagggcca gatcccgctg gagacggtcg tggagtacct ccgcctcatg
     1081 agcgccgagg gcgcggtcga gatcctctga gccgggccgg agcgcaacgc cgcgttccga
     1141 gggagaaatg gcgcgatggg cacgcaggcg acggtgcgac caggagccca aggagaagcc
     1201 gcgcggcggc gcatcgagga ggcctaccgg gcgctgcgcg agaaggatgt gcgttgcacc
     1261 acgctggagc gcgtccgcct cctgatcgag gcggacgcgg cggttcaggg tctcccccag
     1321 ccgctgcagc ttggccacgg cctgacgtac ctcctggagc ggatcgggct gccactctcc
     1381 ccggatgacc agctcctcgg gcgcatccac gaggaggtgc cagatcccga aggtgagcgg
     1441 ctgttccagg aggccgttac gcgctggggt ggtgactcgc gccccgcctg gttgcgcgat
     1501 tcgggccacg agtgtttcgc gtgggagcgc ctgctccgcc tagggctgtc tggattggag
     1561 gcgttcgccc gtagcgagcg tgagcggcgc aaagccgagg gcgcgaagga agcctcgctt
     1621 cacttcctga aaggggccga gttagtctat gccgggctcc gcgcctacgc gcgccgttac
     1681 gcggcggccg cgcgcgccgc tggattggaa gaggccggcg cccgatgcgc ggcgattgcc
     1741 gagcgtcccc cgcgccactt cgccgaggcc ctgcagctga tctggctcgt cggccacgtc
     1801 tactgcacga tggtcgcggc gaatccgacc ctcaccttcg ggcgcctcga cgggctcctc
     1861 ctcccctttt atcgcgagga tctcgccgcc ggcaggctca ctcctgacga ggccggcgac
     1921 ctgatcgagg agttctactg caagaacaac ttgatcctcg ggcggggcga gcatcagatg
     1981 gggggcgacc cggagaagga caccggatgg cgccgcaact tgtgctacga ttccccccag
     2041 tacgttgtgc tcggcgggca ccgacgcgac ggctccccgg tcgccaacga tctcactcgc
     2101 ctcttcctgg aacgcgtggt gcccgggttc aagaacccgg tgatcgtcct ccgctggacg
     2161 gacgacctgc cggagccgct gtggcgcctg gcgtgcgaga agatgcgcgc caacgccagc
     2221 atgatggtgt ataacgacgc ggcggtcatc cccgcgctcg tagacgccgg cattgccgaa
     2281 gaggacgcgg tcacctacac gatgcacggc tgcaactggc ccgacgtgcc ggggatccag
     2341 cggacagtga gccactgcga gccgcccttg ccgaagctct tcctcaccgc gctcctggga
     2401 ggcgagggag agacgccgcc ggtgctgccg tgcatcgaca cgctctacgc gcggttcttc
     2461 gcgctcttcc gccaggaggt cgccaagaag tgcgacgaga tccgcgcgca ccgggcgcgc
     2521 tgggacgagc tcgcccccgg caccctacgc gtggacgact gcttcctgga tggaccggtg
     2581 gcccgggcac gctcgtggca gctaggcggg gtgcagtatc acaccatcac ctgcacgctc
     2641 cactcgctcg ccaccaccgc cgactgcttc gccgtcgtgg atgagctggt gttccgtgcg
     2701 cgccgcgtct cgctggagga gctgcgcgag gcgcttcgga ctgatttcgc cgaatgcgag
     2761 ccactgcgcc ggctctgcca ggaggccccg aagttcggcc gcgatgacga ctgcgccgat
     2821 cgacacgccg tgcgcctgat cgaagggcta ctcgccgcga tcgatgaggc cagcgggcgc
     2881 ggcacgccag aggcggtgac gctctttcgt tgtctggaga cggacatgcg ccaccggctt
     2941 accggggcgc agacgggagc cacgccggat ggccggcatg ccggaatgcc caccagcgag
     3001 aacacctcgc cctcgcctgg ctcctgcacg cgcgggctca cggcgatgct ccattccctc
     3061 gcgaagctgc cgctgcatcg catctgctcc ggcgcgctca acatccggct gcaaccgcgc
     3121 ctggtggcgg gcgaagaggg cctctcgcgc ctggccgcgc tgctccgcgc ctacttccgg
     3181 atgggcgggc tgcaggcgca gctctccctg gcagacacgg ccacgcttcg cgatgcccag
     3241 gagcaacccg aggcgcaccg cgacctggtc gtccggatca ccggctacag cgcccttttc
     3301 accgacatga cgcgcggcgc ccaggaggag atcatccggc gcgaggaaca cgccgcgtaa
     3361 tccgggctca ctccttccct tccggcgtga tccggaagtg gtcgagccag tactcctggc
     3421 ccttcttccc attgccctgc gtgtagaggt agaaggtgcg cagccggcgc acctcggggt
     3481 gaacctcgcg gatagcgcgc acgtcgacca ctacctcgtg ccactcgcca tcatccacca
     3541 ggagcacctt cttcaggtcg cgcgaccggc cgcaggcacg cgccgggctc ccgcccaggc
     3601 agaccatccc gccgcctcgc atcgcctgag ggaacccctc cacccagatc ccgaccggca
     3661 cgcccggcgg aacgcggtag gagaagcgga ccagggggaa gcggtcgata tcccacccgc
     3721 gcgggcggat gagcgcgccc atcacagcgc catcatcggt ggcaaagacg cggagcgagt
     3781 gctttccgga ggccgcggtc gtgttgtcga tgcgcgatgt ggtgttgggc tgccggttga
     3841 ggtaccagcg gtagaaccac tcctcgcggt tctccggctc gaaatcaacg accacggtcg
     3901 gctccgtcgc cgtctccatc gtgggcaggc gcaccccctc gggcacgcgc ggcgcgcggt
     3961 accacgcctc gttctccgcg ccccgctcgt aggcgcccag gtcgggcgct ttcccgacat
     4021 aggggagtga gacgccctcg cccttctccc agctcacctc gcggtccagg aggagcgtat
     4081 tcgcgtccag gtccaccttc acgacgcgcg cctggcgctt ccgtggcccg atgaagatca
     4141 ggtcgccctg ctcgccgggg atgccgaaac cgtcgtagaa cgggcgtgcg tcttccacag
     4201 ggagcgtgcg gccacgcccg gcctcccgcg ttaccgtgag cggcgcgccc gcgtcgaggg
     4261 cggggctatc gggacgcagg cggtagtcgt cgcgctccgc gtccacgaag cgcgggtcgc
     4321 catccaggtt gccggtgaac tgatccgggc aagcggcgtt cgcctccgcc gcggtgcgga
     4381 gtgcaccggg gcgcccgtca ttccaggcat agcggatgga gggcgttccc ggcttcgggc
     4441 cacagaagag gttgtggatg aagcggttgt cgcgcccgat gctcggcccc aggttcaggg
     4501 ccacgccatc gccatccgca tcgttgaacg cgaagaggtt gttgaggagg acattgccgc
     4561 ggaccatgtg tggatcgggg cgagcagaga tgtcgaacat ctcgaagccg gactcgtagt
     4621 tggccacgaa ggtgttctgg tagagccgcg agctgatcat tccgaacggc tccatttgct
     4681 ggtagcgata ggcgtggatg ctgagcggct ggtagtagtt gcggtagacc aggttgcgcc
     4741 ggaagatgcc gtcgcgcgtg aacagcttgg cccggccgtc ggcggagccg ctcccgtgat
     4801 aggtgttggt gatcacgcat ccctcgaaga gcgtgcgcgg cgccgtgaac agctcgaagt
     4861 tgcgccccca gcgcccatcg aagacgcacc cgcggacgac gttgtggtgc gagtcgatcc
     4921 agaggcagaa cggacagtgg cccgcccggc cgaagtggca cttctcgatc acgttgtggc
     4981 tgctcgcgtt gagctggaac atgttgcccg atacatgccc ggtctgcccc agctgcacgg
     5041 atcgccacac ttcgagctcc tcgtagcggt tgtagtggca atcgatcatc cgcgcgggga
     5101 tgtagcttcg agacgcgccc tccatacggc agcggcgcaa gatgcaatac cgcgttgttt
     5161 cgaacagcac ccagtcgccc gcctcgggca gcaggtggaa gccctcgata gtgatgtggt
     5221 cacgctcctt caggcgcacg cagagggaaa gcccatcact cgacttcccg cccgtgagaa
     5281 tcgcccccag ccgctgctcc gagcgatagg tgatcggctt ccccttctcg ccagagacgc
     5341 ccggctcgat cacgccggca taccgcccgg gcaggaaggt ggccgtatcg ccgggccgga
     5401 gcgtctcatt cgcgcgcgcg atgctccgcc acggacgggc gcgcgtgcct gggttctcat
     5461 cgtttccatc gggcgacagg aagtactccg cgccgagcgc cggcacgcag agaagcgcca
     5521 tcccgaggag caacaggatt cggccggttg tctccattct ttctcccttt caccgcagca
     5581 ctggcttcgc cgacgcggtc tcctccatca gagcatcggc cacatcctgc gcggatcggc
     5641 gaacggggtg gagggagagg tcggcgcagg cgaggagcac caggaaggcc cccgtgtaga
     5701 aaccggcacc ctgatgctct ccgaagtaga ggatcccgat cgccgcggta agcacagggc
     5761 tcagaaggga gagcaggctg ccataggcgg cgccggtgaa tttgtaggcg taggtcatcg
     5821 tcagctgccc ggcggtggcc agtaggccga tcgccaggag caccagccac tcggccggcg
     5881 tggggggcga ccagacgcgc gccgccggcc aggcgaccag gacgatcccg aagatcgact
     5941 gcgaccagaa gatgttgctc gcgctatccg tccgccggca ccgggtgacg cagacgatgg
     6001 caaaccccgc cgcgatcccg gaaacgagcg cgaccaggtc gccatacgag acggcctgca
     6061 tccccacccc gcacaggagt ccggtgccca ccgccgcggt gaagatggcg acccactgcc
     6121 ggagtccgac acgctctccc aggagaggca ccgcgaagag cgcggcgaag atcacgtagg
     6181 tgtggtggag cgtcacggcg cgcgccagcc caacctcctg gatcgcccag tacgagagga
     6241 tgcaggcaat ccccccgctt actccccggg caacgatcca gggccagttc ctccagcgca
     6301 tcttctcgag gccgaacacg aagaacagcg ccacgagcac cgtgccgacg acaaaccgcg
     6361 ccagcaccat cgtgtaagag tggaccccgg tcgcgccgcg caccaacgcc gacatggcgg
     6421 cgaagaacac ggccgagaga agcatcatcc agatgccctt gcgtgcgatc atcacctgcc
     6481 gttcgccctc atcccttcca gaagcagctt cacccgcgcg aattcacgac gcgggtgtag
     6541 catcgcgcag gcagagactc ctgcgctcct tctgagtaaa cggccctacg ggcctgttcg
     6601 gaccagcccg tagggcttcg ctcgcgggca gcgcggatct tgggggtccg cgcctgattc
     6661 tgccgggtag cagcgggggt tttcaggccc gcgccgatgg ggctgccgag gcctgcgcgg
     6721 ccaagcgcca cccgggctca tcctggtcac gatgcagtgg tcgacgcttc gcgcgcgctc
     6781 cccgcttcct tcttccggcc ggctttcaag taggtgaccg tcatccaccc cacgatgccc
     6841 agggcaacca ccagtgccac cgcgaagatg gcgaagacgc cccattgcgg ctggacagcc
     6901 agcgtctccg ggttgaagta cggcgcgacg tagctcagcc gaacctggtg ccttaggatg
     6961 gccatgcatg ccagcgcaac tgcaatggcg gcggtgccaa gccacaggtg gacgcgcagt
     7021 agcccgatcg cagccacacc cagtaggatg ccaagcccga gcagaaggag cgagggcgtc
     7081 attccgcccg agaagaggtc gagcgccggc ttgggaagcg agaagaggaa ccaggtgcca
     7141 accgccaggt tcacggcggt ggcgcccgtg aagaggccga tgccctgccg gcgcatccag
     7201 gtggcgaact ccggggcaac cggggcctgg gcccgagaga gcacagcgat ccagacaccg
     7261 gtcacggcca gggcgcccac cagcatatgc gcgaaccgag gccacaccac cgggtcggtc
     7321 acattgacat acagccccgc cgcgctttgg gcgtagatgc cgctccactt ctccggcgag
     7381 agcatcagca ggacgttcat cacccacagg aagccgatag cggcagccgc cacggcactc
     7441 gctgtcgcca cccacggttg atgcttcccg agcgcccgcg gcgccagcgc gcaggcgtag
     7501 tagccataat agccagcgat cagcagcggg atcacgccca tccagagcca cgccatcagc
     7561 accgacgacg tgtagaacag ctgtccatag accacctgaa cgaagaggag cggcgcgacc
     7621 ccggtggtga tggtgaacgc cgtcactgac gggagcgccc gggcaatcac ctcgctcaac
     7681 cggcgctggt tctcatcccg gcggccgcgg aaagcggcca ccgcggcgag gatacctccg
     7741 ccgagggtga agctcatcgg caccagatgg agcgtgaagg tgagcagcag taggaaatga
     7801 accagccaga taggcgccgg caccggcact gcgtcgggga gcggaatgat cggcatcatc
     7861 gcttcacctc cgtaacgggc agctcggccg cgggggccag ggcattggcc gcggggcgtc
     7921 catccacgcc cagcgcgtcc ggcggcggcg agggcggccc agccaggcgg gcgagccatg
     7981 ccgccagcgc agcccgctcc tcgggcgttc ccatgaaggg cggcatgtaa ggcttcagcg
     8041 tactcaggcg cgaaagctgg tagtccgtgt acgcctcatc ccagccggcc atcaggggaa
     8101 gcacgccgtt atacccgcgc agcgtgtggc agcttctgca ctggagccgg aacacctcct
     8161 cgccggcggc gagacggtta tgcggagtca cttccttctc gctgctccat agcgcgctct
     8221 tcagcacccc tgtgcgagtc accgcgt
//
LOCUS       DAOSCV010000054         8246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5643_length_8246_cov_3.324991, whole genome shotgun sequence.
ACCESSION   DAOSCV010000054 DAOSCV010000000
VERSION     DAOSCV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_8246_cov_3.324991"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2848)
                     /locus_tag="PLY56_02380"
     CDS             complement(<1..2848)
                     /locus_tag="PLY56_02380"
                     /inference="COORDINATES: protein motif:HMM:NF022752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HOJ20357.1"
                     /translation="MPKPLNAGHRIPSTILKLLAATALAFLTTSHALSAPATRQAGGS
                     WVLENAMLRVEIDPPAGRFQVLDKRARFTWRGPDGDASQATRILLPKVTTPPKIDGDL
                     SEWAHIGTPLTIPEGKEGADPGARLRAAWGPAGLYLAVAVKDRRFVTCAPDETRWWEK
                     DSVEFWIHETQYAVRFGTWGANIWAASGDVRDASVDFAETADGYTVEVLMPARLLGAA
                     KSPGGLFRLAVGVNDADDANGRKAQIYFPASWQHSNSETFAIAVLGDEKGEMPPVAGD
                     AKPVLTPVYGKQAPGTASFTGSVRTKGQEVPVTTTFALLGDTPELRITLAPADPAAPV
                     DKMRVLHPLVLDDPEGRVLATPYNGGIGIPTDDLSWRGREWRLSGMLDMPWVGLTDGT
                     KGYLLLWELPRSCDDGLARLEGVLVGGKTLLAPAAWHAGTKGTFAYPRVIRYIFIPGG
                     GHVAMCKRFREYAREHGILKTLREKMKRKPQLARLAGAPDVWGRSDLKFCREAKAAGI
                     DRLLVNGTQPKADTEAIKALGYLAGRYDNYEDAYEGDPGHMGDFKTETDGLVLANGER
                     MKAWLTHGTPPKQFMKRCTALFEKVARTWIPRELEAHPMNARFLDVTTACGLAECYSD
                     HHPLNRTEDRLARRRLAQYVGNELGLVLGGEHGRWWAADLYDYWEGMQSGGFYSWPAG
                     YVGEKIPATREEIGQRYLEWGLGERYRYPLWELVFHDCVVSTWYWGDSTGHLIQAAPE
                     LGYKKDAFNILYGTIPLYWVNKPYSYRWDQPETRARLLESYRNTCKLHEQIAFEEMVS
                     HAFVTPDRAVQTTRFADGTQVWVNFGEKPWTLKRDGKEWTLPQYGFYVKGPRIEQYRI
                     VKGDAPNGTITCIRTPDYLYAEGNVPGAVETAEGTGVTIRRDGPDRLRIIPEQATRWV
                     RLNPSALFAASAEKKAAASPGQWRIVQLDEEGNAL"
     gene            3188..4270
                     /locus_tag="PLY56_02385"
     CDS             3188..4270
                     /locus_tag="PLY56_02385"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ20358.1"
                     /translation="MTDRTCPVPSGGPASLRQVEEAVRREFDAGLFTGAVVLLGDRER
                     VLYHEAFGLAQVEPEPVAMRRDSIFDVASVTKVVATATACAICVDEGRLDPDAPLTDY
                     IPDHRGRGVERITLRHLASHTSGFGAARQIGRRASGDAFFQALLDENPTREPGTRHEY
                     ACYNAILLGIIVERVTGMRFGEFCTRRIFRPLGMSESVFNVVPPSERVVGGPRGVPLG
                     ISHNLDSRMAGRAIGNAGLFTTAADLARFARMMLGGGELGDTRILSERVIDDVTQPWT
                     GPDFPEWGFLWCLNQRAEHRPSRLSPRAYGHGGFTGQSLWIDPEQGLFLLVLTNRIHP
                     EYGTGCKPEQDRARARIADALLEVFA"
     gene            complement(4287..5333)
                     /gene="selD"
                     /locus_tag="PLY56_02390"
     CDS             complement(4287..5333)
                     /gene="selD"
                     /locus_tag="PLY56_02390"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOJ20359.1"
                     /translation="MTREEIRLTSMSRAAGCAAKAGPEALAHVLRPLQALFPAERFPA
                     LQVGLEVTDDAAVYRITDDLAIIQTVDFFTPIVDDPHDYGAIAAANAMSDVYAMGGEV
                     VLALNVCCLPGTLPEEIAAEILRGGAEKVLEAGGVLAGGHTVDDDEPKYGLAVMGTVH
                     PDRVLTKGGARPGDALVLTKPLGVGMITTALKAGVADPAHIGPAVDSMKKLNREASRL
                     IREAGAHAATDITGFSLLGHAWEMAERSGARLRFEFDRLPFLEGARRYAEEWLFPGGT
                     CRNEKAYASHVTFADRIPEEMRQLLFTPETSGGLLAAIPPEKAELLEARFAEAGHFFR
                     RVGEVTEGTGVTVE"
     gene            complement(5330..5926)
                     /gene="yedF"
                     /locus_tag="PLY56_02395"
     CDS             complement(5330..5926)
                     /gene="yedF"
                     /locus_tag="PLY56_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOJ20360.1"
                     /translation="MMAKVVDARGLPCPQPVIATRNALRESEAVTVILDDEIARQNVT
                     RMAERAGYRVEISTRDDGIYLQIYKEGATPEPVSAPEAAPSAGGPLVLLVASECIGRG
                     DDELGRILARSFFHALCEAEPLPERIFFLNSGVKLAVEGSPVLDDLRALSERGVTLLA
                     CGTCLEFYGLKEKLAVGEITNMYAIAEALMGAGKVVRL"
     gene            complement(5967..6194)
                     /locus_tag="PLY56_02400"
     CDS             complement(5967..6194)
                     /locus_tag="PLY56_02400"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOJ20361.1"
                     /translation="MNAGAYTLLLVGSTQRALRAEQLLQEAGIACKLIPVPRQIRSDC
                     GLCLRVARADSAAALQVLRAAGLDEVTAHDL"
     gene            complement(6191..7441)
                     /locus_tag="PLY56_02405"
     CDS             complement(6191..7441)
                     /locus_tag="PLY56_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ20362.1"
                     /translation="MLSRAGCLAKEERRSAAKSLTTMAEGPDARSACPALIYFDNAAT
                     SWPKPPGVAAAMARYLEEVGASPGRSGHRLAIAAARALFSAREAVAQLFHFDDPLRVV
                     FATNATEALNLSLTGLLRPGDHVVTSSVEHNSMMRPLRALQQGGVEVTVVPCSREGVL
                     DPNRLEFAISPRTRLIALTHASNVTGTLLPVAEAGQLARERGALLLVDAAQTAGAYPI
                     DLTTDPIDLLAFTGHKSLYGPTGTGGLVLGERVDPSQLQPLIRGGTGSHSEHEEQPEF
                     LPDRYESGTPNAVGLAGLDAGVRWVLERGVERIRAHEVALSRQLIEGLSAIPGVTLYG
                     TRDAERQTATLSFTIAGKDPAEVSVRLDENFGILCRAGLHCAPAAHRTLGTFPQGTIR
                     FSLGAFNTPEEVATAVAAVVEVAR"
     gene            7513..>8246
                     /locus_tag="PLY56_02410"
     CDS             7513..>8246
                     /locus_tag="PLY56_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20363.1"
                     /translation="MMRAKAWFFSILVSAALCSGVQGATVDAGNGFRATWEGQAIRSL
                     SVRGKPLPGVAASFQLVDPRTKASADPAKFRVEAKLAARNGALWLDGVVVAAGTEDTV
                     VDLNLRMEGVTLPLGDGVENPLLLPARLLNKLPIASLRTLATGSDDLGIGLHADRLCV
                     AEFRALPEEKAVTLRFPFGFSRHARPEMRMRAPFSVVLYPTDPRWHFRSALAGYYRLF
                     PDHFQRRTDRHGGWFFANETKNIPNP"
BASE COUNT         1239 a         2783 c         2822 g         1402 t
ORIGIN      
        1 ggagggcgtt cccttcctca tcgagttgga cgatgcgcca ctggcccggg ctggccgccg
       61 ccttcttttc cgcgctggcg gcgaagagtg cgctcggatt caggcggacc caccgtgtgg
      121 cctgctccgg gatgatgcgc agccggtccg ggccgtcgcg gcggatggtg acgccggtgc
      181 cctcggccgt ctccacggcg ccgggcacgt tcccctcggc gtagaggtag tccggcgtgc
      241 ggatgcacgt gatggtgccg ttcggggcgt cgcccttcac gatccgatac tgctcgatgc
      301 gcggcccctt cacgtagaag ccatactgcg gcaacgtcca ctccttgccg tcccgcttca
      361 gcgtccacgg cttctcgccg aagttcaccc atacctgggt gccgtcggcg aagcgcgtgg
      421 tctgcacggc gcggtcgggg gtgacgaagg cgtggctcac catctcctcg aacgcgatct
      481 gttcatgcag cttgcaggtg ttgcgatagc tctccagcag gcgcgcccgc gtctcgggct
      541 ggtcccagcg gtagctgtag ggcttgttga cccagtagag cgggatggtg ccgtagagga
      601 tgttgaaggc atccttcttg tagccgagct ccggagccgc ctggatcagg tggccggtgc
      661 tatcgcccca gtaccacgtg gagacgacgc aatcgtggaa aaccagctcc cagaggggat
      721 agcgatagcg ctctccaagg ccccactcca ggtaacgttg gccgatctcc tcgcgggttg
      781 cgggaatctt ctcgccgaca tagcccgcgg gccatgagta gaagccgcca ctctgcatcc
      841 cctcccagta atcatagaga tcggcggccc accagcgccc gtgctcgcca cccaggacca
      901 gtccgagctc gttgcccacg tattgggcga ggcggcggcg cgcgagccgg tcttcggtgc
      961 ggttcagtgg gtggtggtcg ctatagcact ccgccagccc gcacgcggtg gtcacgtcga
     1021 ggaagcgggc gttcatcggg tgggcctcca gctcgcgcgg gatccaggtg cgcgccacct
     1081 tctcgaagag cgctgtgcac cgcttcataa actgcttggg gggcgtgccg tgggtgagcc
     1141 aggccttcat tcgctcgccg ttggcgagga ccaggccatc ggtctctgtt ttgaagtcgc
     1201 ccatatggcc cgggtcgccc tcgtaggcat cctcgtagtt gtcataccgt ccggcgaggt
     1261 agcccaacgc cttgatcgcc tcggtatccg ccttgggttg cgtgccgttc accagcagcc
     1321 ggtcgatccc ggcggccttc gcctcgcggc agaacttcag gtcgctgcgg ccccacacgt
     1381 cgggggcgcc ggccaggcgc gcgagttgcg gcttgcgctt catcttctcg cgcagagtct
     1441 tcaggatgcc atgctcgcgc gcatattcgc ggaagcgctt gcacatcgcc acatgcccgc
     1501 cgcccgggat gaagatgtag cggatcaccc gggggtaggc aaacgtgccc ttggtgcctg
     1561 catgccacgc cgccggcgcc agaagagtct tgcctccgac gagcacgcct tccagacgcg
     1621 cgaggccatc atcacacgat cgggggagct cccagaggag cagatagccc ttcgtgccat
     1681 cggtgaggcc tacccacggc atgtcgagca tcccactcag tcgccactcg cggcccctcc
     1741 aggagaggtc atcggtcgga atgccgatgc cgccattgta gggcgttgcc agaacgcggc
     1801 cctccgggtc gtcgaggacg agcggatgca agacgcgcat cttgtccacc ggagcggcgg
     1861 gatcggcggg ggccagggtg atgcgcagct cgggcgtgtc gccaaggagg gcgaaggtgg
     1921 tggtgaccgg cacctcctgg ccctttgtgc gcacgctgcc ggtgaaggat gcggtgccgg
     1981 gcgcctgctt cccataaact ggggtgagca ctggttttgc atcgccagcg accggcggca
     2041 tctcgccctt ttcatcgccc aagacggcga tggcgaacgt ctcggagttg gagtgctgcc
     2101 acgacgcggg gaagtaaatc tgcgccttgc gtccgttcgc gtcgtcggca tcgtttacgc
     2161 cgacggccag acgaaacagg cctccggggc ttttcgctgc gcccaggaga cgcgcgggca
     2221 tcagcacctc caccgtgtag ccatcggccg tctccgcgaa gtccaccgag gcatcgcgaa
     2281 cgtctccaga cgcggcccag atgttggcgc cccacgtgcc gaagcgcacg gcgtactgcg
     2341 tctcatggat ccagaactcg acgctgtctt tctcccacca cctcgtctca tccggggcgc
     2401 aagtgacgaa gcggcgatcc ttcacggcaa ccgccaggta gagcccggcc gggccccacg
     2461 cggcgcgcaa gcgggcgccc ggatcggccc cctccttccc ttccgggatg gtgagcgggg
     2521 ttccgatgtg ggcccattcg gagaggtcgc catcaatctt ggggggagtg gtcaccttcg
     2581 gaaggaggat ccgggttgcc tgactcgcgt cgccgtccgg cccgcgccag gtgaaacgag
     2641 cgcgcttgtc gagtacctgg aagcgcccgg ccggagggtc gatctccacg cgcagcatcg
     2701 cgttctcgag cacccaggat ccgcctgcct gtcttgtggc tggcgctgac agcgcgtggc
     2761 tggtggttag gaaggcaagt gcggtagccg cgagcaactt gaggatcgtg gaagggattc
     2821 tgtgcccggc gttcaacggc tttggcatga ttggcctcct actctcccga gcggaccgga
     2881 tgcgtcttgc ttgtcgcggg cgtggccagc acgcgcgcag ggtgcgaact ccgctttgat
     2941 gcggtccatg gcggctcggg cgggtgccga gagcacgctc gagcgccacc tgcctggcgg
     3001 cgccgcaggg cctggcctga cgcccggtga tagagtacac cacagcaagc cccacctcct
     3061 gctccgtttc ccgcacgccg aggggatgct ttctttccag aaggagggcg tgcgcgccgc
     3121 atggaagcca gttttagtgc ttgccctctc tacccgttcg aggcgcagtt tcggcatgga
     3181 ggagcagatg accgatcgaa cctgtccggt accatctggc ggcccggctt cactgcggca
     3241 ggttgaagaa gcggtccggc gcgagtttga cgcggggctg ttcaccggcg cggtggtgtt
     3301 acttggcgac cgcgagcgtg tcctttacca tgaggcgttt ggcctggccc aggtggagcc
     3361 ggagcccgtg gcgatgcgcc gcgattcgat cttcgacgtg gcgagcgtca ccaaagttgt
     3421 ggccacggcg acggcgtgcg caatctgcgt ggatgaaggc cgcctggatc ccgacgcgcc
     3481 gctgaccgac tacatcccgg accatcgggg ccggggcgtc gagcggatta cgctgcgcca
     3541 cctggcgtcg cacacctcgg ggtttggtgc cgcgcgccag attggccgtc gggcaagtgg
     3601 cgacgcgttc ttccaggcgc ttttggacga gaatcctacc cgcgagccgg gcacgcgcca
     3661 cgagtacgcg tgctacaacg ccatcctcct cggcatcatc gtggagcgcg tcaccgggat
     3721 gcgcttcggc gagttctgca cccggcggat cttccggcca ctggggatga gcgagagcgt
     3781 cttcaacgtg gtgcctccat ccgagcgcgt ggtcggcggc ccgcgcggcg ttcccctggg
     3841 gatctctcac aacctcgata gccggatggc cgggcgggcg atcggcaatg ccggcctctt
     3901 caccacggcg gccgacctgg cccgcttcgc tcgcatgatg cttggcggcg gggagctggg
     3961 cgacacccgg atcctctcgg agcgcgtgat cgacgacgtc acccagccgt ggaccgggcc
     4021 ggacttcccg gagtggggct tcctctggtg cctcaaccag cgtgccgagc accgcccgtc
     4081 gcggctctcg ccgcgcgctt atgggcatgg gggctttacc gggcagtcgc tctggatcga
     4141 cccggagcag gggctctttc tccttgtcct cacgaatcgg atccacccgg agtacggcac
     4201 gggctgcaag ccggaacagg accgcgcccg tgcgcgcatc gccgacgctc tcctggaggt
     4261 gttcgcgtag cgtcgggaga cgcggctcac tccaccgtga cgccggtgcc ttcggtcact
     4321 tcgccgacgc ggcggaagaa gtggccggcc tcggcgaagc gcgcttctag caattcggcc
     4381 ttctcggggg gaatggcggc gaggaggcca ccggaagtct ccggggtgaa gaggagctgg
     4441 cgcatctcct cagggatgcg gtccgcgaac gtgacgtggg aggcatacgc cttctcgttg
     4501 cggcaggtgc ccccggggaa gagccactct tccgcgtagc ggcgcgcccc ctccaggaag
     4561 gggagccggt cgaactcgaa gcgcaggcgc gccccactcc gctcggccat ctcccaggcg
     4621 tggcccagca gcgagaagcc ggtgatatcc gtggcggcat gcgcgccggc ctcccggatg
     4681 agccgcgatg cctcgcggtt cagcttcttc atggagtcga ccgctgggcc gatgtgcgcc
     4741 gggtcagcga cgccggcttt gagcgccgtc gtgatcatcc ccacgcccag cggcttcgtc
     4801 agcacgagtg cgtctcccgg gcgcgccccg cctttggtga ggacgcggtc ggggtggacg
     4861 gtgcccatga cggccaggcc gtacttcggc tcgtcgtcat cgacggtgtg cccgccagcc
     4921 agcacgccac ccgcctcgag cactttctcg gcgcccccgc gcaagatctc ggcggcgatc
     4981 tcctccggaa gcgtgcccgg caggcagcag acgttgagcg ccagcacgac ctcgccgccc
     5041 atggcgtaga cgtcgctcat cgcgttggcc gcggcgatcg cgccgtagtc gtgcgggtca
     5101 tcgacgattg gcgtgaagaa atcgaccgtc tgaatgatgg cgagatcgtc ggtgatccga
     5161 tagaccgcgg cgtcgtcggt cacctccagg ccaacctgta gcgccgggaa gcgctcggcc
     5221 gggaacaggg cttgcagcgg gcgcagcacg tgcgccaggg cctctgggcc tgctttcgcg
     5281 gcacaaccgg ccgcccggct catcgatgtc agacgaatct cttctcgcgt catagcctca
     5341 ccactttgcc ggcgcccatc agcgcctcgg caatcgcgta catattcgtg atttcgccaa
     5401 cggcgagttt ctccttcagc ccgtagaact ccaggcaggt gccgcaggcg aggagggtga
     5461 ccccgcgctc ggacagggcg cgcaggtcgt ccagcaccgg cgagccctcg acggcgagct
     5521 tcaccccgct attgaggaag aagatcctct ctggaagcgg ctccgcctcg cacagcgcgt
     5581 gaaagaagga tcgggcaagg attcgcccca gctcgtcatc gccgcggccg atgcattcgc
     5641 tggcgaccag gaggaccagc gggcctcccg ccgatggcgc ggcttcgggc gcggatacgg
     5701 gctctggcgt ggcgccttcc ttgtaaatct ggagatagat gccgtcgtcg cgcgtgctta
     5761 tttccacgcg atacccggcc cgctcggcca tgcgcgtcac gttctgccgg gcgatctcgt
     5821 catccaggat cacggtgacg gcttcgctct cgcgcaaggc gttgcgcgtt gctatcaccg
     5881 gttgcgggca cggcaacccg cgcgcgtcga ctacctttgc catcattgcc tcctctcgtg
     5941 ccgcgccagg cgcggccact ccgagattag agatcgtgcg cggtcacttc atctaggccg
     6001 gcggcgcgca gcacctgcag cgccgcggca gaatcggcgc gggccacgcg caggcagagg
     6061 ccgcaatccg agcggatctg gcgcggcacc gggatcagct tgcacgcgat cccggcctcc
     6121 tgaaggagct gctcggcacg cagcgcgcgc tgggtggagc ccaccaggag aagggtgtag
     6181 gcccccgcgt tcaccgcgcc acctccacca cggcggccac cgcggtggcg acctcctcgg
     6241 gcgtgttgaa ggcgcccagg ctgaaccgga tcgtgccttg cgggaaggtg ccgagcgtcc
     6301 ggtgcgccgc cggagcgcag tgcagcccgg cccggcagag gatgccgaag ttctcatcga
     6361 ggcgcacgct cacctccgcg gggtccttgc cggcaatcgt gaaggagagg gtggccgtct
     6421 gccgctccgc gtcgcgcgtg ccgtagagtg tcactcctgg aattgctgag agcccctcga
     6481 tcaattggcg ggagagggct acctcgtgcg cgcggatcct ctccacaccg cgttcaagca
     6541 cccagcgaac gcctgcgtcc agcccggcca agcccaccgc gttgggcgtg ccgctctcgt
     6601 aacggtccgg caggaactcg ggctgctcct cgtgctcgga gtggctaccg gtgccgccgc
     6661 ggatcagcgg ctggagctgc gatgggtcca cgcgctcgcc caggaccagc ccgccagtgc
     6721 cggtgggtcc gtagagcgac ttgtgcccgg tgaaggcgag caggtcgatg ggatcagtgg
     6781 tgaggtcgat cgggtaggcg ccggcagtct gagcggcatc gaccaggagg agcgcgccgc
     6841 gctcgcgggc gagctggccg gcctcggcga cgggcaggag cgtgcccgtg acgttggatg
     6901 cgtgggtgag cgcgatcagc cgcgtccggg gagagatcgc gaactccagc cggttgggat
     6961 cgagcacgcc ctcgcgcgag cagggcacca ccgtcacctc caccccgcct tgctgcaggg
     7021 cgcggagcgg gcgcatcatg gagttgtgct ccacgctgct ggtgaccacg tggtccccgg
     7081 ggcgcagcag cccggtcagt gacaggttta gcgcctccgt ggcgttggtt gcgaagacga
     7141 cgcgcagcgg gtcatcgaag tggaagagct gggccaccgc ttcgcgcgcg gaaaagaggg
     7201 cgcgcgcggc cgcaatcgcc aggcgatgcc ccgagcgccc cgggctggcg cccacctcct
     7261 cgaggtagcg cgccattgcc gcggcaacgc ccggtggctt cggccacgac gtcgcggcgt
     7321 tatcgaagta gattagggcg gggcaagctg agcgtgcatc cgggccttct gccatcgtag
     7381 tgagagactt cgcggcggag cgccgttcct ccttcgccag acaccccgcg cgtgagagca
     7441 cccgcgcgct gctcgaccgc ctgcccaacg ctcagaggag gaaaccgaaa gaccctgtcg
     7501 aaccagggca gcatgatgag agcaaaggcc tggttcttca gcattctcgt atctgccgcg
     7561 ctctgcagcg gcgtccaagg ggcaaccgtc gatgccggaa acggcttccg cgcgacctgg
     7621 gaagggcagg cgatccgttc cctcagcgtg aggggcaagc cgctccccgg cgtggccgct
     7681 tcgttccagc tggtcgaccc gaggacgaaa gcgtctgcgg acccggcgaa gttccgcgtc
     7741 gaggcgaagc ttgcggctcg gaacggagcc ctctggttgg atggtgtcgt cgttgccgcc
     7801 ggcaccgagg acaccgtggt cgacctgaac ctccggatgg aaggcgtgac gcttccgctg
     7861 ggagacggcg tcgagaaccc gctgttgctt cccgcccggc tgctcaacaa gctgccgatt
     7921 gcgtcgctgc gcacgctggc cacggggagc gatgacctag gcatcggcct ccatgccgac
     7981 cggctctgcg tcgcggagtt ccgggcgctt cccgaggaga aagcggtgac gctccgcttc
     8041 ccgttcggct tcagccgcca tgctcgcccg gagatgcgca tgagggctcc attctcggtg
     8101 gtcctctatc ccacggatcc gcggtggcac ttccgctcgg cgctggccgg ctattatcgc
     8161 ctcttccccg accatttcca gcggcgcacc gaccggcatg gcggctggtt ctttgccaac
     8221 gagacgaaga acatccccaa ccccca
//
LOCUS       DAOSCV010000055         8234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5653_length_8234_cov_3.699719, whole genome shotgun sequence.
ACCESSION   DAOSCV010000055 DAOSCV010000000
VERSION     DAOSCV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8234
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_8234_cov_3.699719"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..670)
                     /locus_tag="PLY56_02415"
     CDS             complement(134..670)
                     /locus_tag="PLY56_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20364.1"
                     /translation="MATAHELFNEYQDPEEAQGLFEEMRQVRSCISTPADAVAQILRF
                     RQEVGPMMIGGRPTCFCANVWEACHQIEAMGRRRLRWAVPLLARLLFHQDPDFDACPE
                     YAVRLQAAAARALGEIGDPRAIPPLLAKLEEGPTYAPLAAGDEVLTGIRIELDEATVA
                     AMAALDALGAALVDGPTC"
     gene            complement(863..2731)
                     /locus_tag="PLY56_02420"
     CDS             complement(863..2731)
                     /locus_tag="PLY56_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ20365.1"
                     /translation="MPIIPTVGRRSPKMRLIIAMLYTVLTLGAITMVYPFLIMVSTSF
                     TSEVDSDEFAPVPSYFYDDAMLFRKYVEAKYNEDIPKFNAYLSNDIPEFKKVEVPANI
                     NQQMVDDWLAFKKTLPDTYKMLGGTTNLSRITPEMQSRYQAWLRERYKGDLQAVNRAY
                     TESNQVWEEVLIPVEDWNQRQYQPVINRKYKEFLEFRAQQPERYFNTASGDGLFQDWL
                     RLRYGTEPDVLNETWGTNYKSRFEVALSERVPSHPKQAADWEEFVRKELPAQFVGADP
                     EAEPLYRRFLAQQYEGNIQVLNARYGTKYAGFHEIPLPAPRKAPVSGMPQADWIAFVE
                     RVLPTKMIYLQTPEILYRAHLKTKYGSVEALNKAWGVSLASFQQVRPPYLEADWVEMQ
                     ADRKAVKREFLVRNYREVFNYIVLHGRSLWNTMILVLGLMSVTLIVNPLCAYGLSRFK
                     LPQTYKILLFLLATMSFPAEVSAIPRFLLLKQFHLLNTYWALILPAMANGYSIFLLKG
                     FFDSLPSELYEAATIDGAGETLMFTKITVPLSLPVMAVIALQTFTSAYGSFMWALLVC
                     QNPKMWTLMVWLSQMQQWAPTFVMFAALVLAAIPTLFVFILCQNVIMRGIIIPTEK"
     gene            complement(2821..5301)
                     /locus_tag="PLY56_02425"
     CDS             complement(2821..5301)
                     /locus_tag="PLY56_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ20366.1"
                     /translation="MPLAYRSLFGFLALWVLAFGASAAQAAPVTVKVWQLPNREARTV
                     DQRADLAVLQRFMELHPEIEMDGFRGITAPGMGQDITPLLAMAGGVAPDVLYVNFRIS
                     ENYIAQGFLAPLDEYVLEWATELAGRPIRTIEEAQPVLSHIIKPQVWPVIYREGPGHE
                     KHVWAIPYGTVVQTLAYRKDLFRKAGLDPDRPPKDWDELFEYAAKMTNPEEGEYGIGI
                     AAGPQASWHFINFLWSAGGEAVVEENGQWRAVYNTPEAVKALQFYQKLNQGEYIRNGK
                     KTKGVAYRGTDVYTKWTLGKLGMYFQYMNDQVMSQVNPNLIGVGPMPMGPSGKRGNEI
                     NATMQGLNATIKDPRVRRAAWEWIKFRAGDEAKRIRTRIYMEAGYAQYLNPEYLKRYL
                     TPEEYHRFSRQVPPGWAETLEEALNSGRPEPYGKNCEMIYTEMTPPIEQAWISTNTDP
                     KYLKALLDKSVAETNVKLMGLLPPGVKEKRRLVAGMVVFGLIIGFAFVFRTVMRTFAK
                     ELEPPPVTGKGWRRFGYAWIVMLPAIISVAVWQYYPVARGSLMAFQDYKLVLPTTWVG
                     LDNFAEVLFAPAFWLGLKNSARYMIYSLAFGFLTPIALALALNEVPRGKVLFRTLYYL
                     PAVTTGLVIFMLWKQFYDRTPSGLLNKVIAGAVAAVNAVITQVGANPIIYTPQDWLGD
                     PRWAMMMIIIPLVWAGMGPGCLIYLAALKSIPEEMYEAADLDGAGLLSKIWRITVPYL
                     RPLIIINFVGAFIAAAQAFDAVFIMTGGGPVNTTRVIGLEIFYTAYIYQKFGYAVAMA
                     WIVGALLVGFTVLQLRILSRVQFKTAQA"
     gene            5808..6863
                     /locus_tag="PLY56_02430"
     CDS             5808..6863
                     /locus_tag="PLY56_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOJ20367.1"
                     /translation="MSRALPALFRRFPQAREHLPWIPLGDLPTPLESLGNLRGRAAPA
                     SVWVKRDDRSSRLYGGNKVRALEFLLADALCRRVDRVLTAGAIGSHFVSATALFSRRV
                     GLRAEAILWSQPWSERCTEALDLLHRLDVPVRSYAHPAIAVAALILSGVQHRLSGRRV
                     ALFPPGGSSPRGALGFVDAALEVAEQVAAGEIPPPESIWVACGSGGSAAGLALGLSLA
                     QLTTRVRAVRVTPRLACNRHRLLGLAAGAWRLLCQGMEAETEGAAPGATHAGEAPAGG
                     PRFGDRLELVEGFYPPGYARETPASRDAAELLTHIGLPGDTTYTGRAMAALLQRAPLE
                     GGTHLYWHTLGSEGAQG"
     gene            complement(6930..7622)
                     /locus_tag="PLY56_02435"
     CDS             complement(6930..7622)
                     /locus_tag="PLY56_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20368.1"
                     /translation="MGPSSSISTGSTARPHSRGFTLIELLVVIAIIAILAAILFPVFA
                     RARENARKSTCQSNLKQIGTAFLQYAQDYDERFPIYNQAGVGAFVVASPYLKNTDVLV
                     CPSSRQNTYGVNYTGVFNANMALSQVQVPSQCIMALDNGKSRALAADPWTRFPPLAGD
                     NAAQGHANDDWAWPDGNATPTSGTLYTDTANPRHADGANCVFMDGHVKWYAIQKLTTP
                     AATFNDCLWLAW"
     gene            complement(7819..>8234)
                     /locus_tag="PLY56_02440"
     CDS             complement(7819..>8234)
                     /locus_tag="PLY56_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20369.1"
                     /translation="LAYLNARSDVATSLYEEIAAVARGEGVRHVRTSLPLPGEMALTG
                     LEPSRVLVASDRATAPLPDPDAPERLQDTRSYLRPGMLLLAAVPPEEIGTGEALRERL
                     HACRDAGADGFTFPPYGLLREHQVRAIGEARSAWI"
BASE COUNT         1462 a         2676 c         2623 g         1473 t
ORIGIN      
        1 cgacgtggag atcgccggcg cgaagaagct ccgcctctac tgcacggacg ccggcgataa
       61 catcaactcg gatcacgcgg cgtgggccga ggcccggctg gagtaggcgc tcccgcgccg
      121 cgccatcccg ggctcagcaa gtagggccgt cgaccagggc cgcgccgaga gcatccaggg
      181 cggccatcgc ggcgacggtg gcctcgtcca gctcgatgcg aatcccggtg agaacctcgt
      241 ctccggcagc gaggggcgcg taggtgggcc cctcctccag ctttgcgaga agcggtggaa
      301 ttgcgcgcgg gtcgccgatc tcgccgaggg ctcgggcagc ggctgcctgg agacgcactg
      361 cgtactccgg gcaggcatcg aaatcggggt cttggtggaa gaggaggcgc gccagcaggg
      421 gcacggccca acgcaggcgg cgccggccca tcgcctcgat ctggtggcaa gcttcccaga
      481 cattggcgca gaagcacgtc ggccggccgc cgatcatcat ggggcccacc tcctgacgaa
      541 agcgcaggat ctgcgccacg gcatcggcag gcgtcgagat acaggaccgc acctggcgca
      601 tctcctcgaa gaggccctgc gcctcctccg gatcctggta ctcgttgaag agctcatgtg
      661 ccgtggccat cttcagctgc tcccagaacc gctcggcatc tcccctctcc cattatacca
      721 atccgcgtga gagtgtgcaa tccgaaggca taccgcgcgg gtggtacgca gcgcggcccc
      781 ggcgcctccc ccatagggag gtgtcggggc cacacgccgt tctgtggatc caacgcggcc
      841 tgccttccgg cagcccaacg cactacttct ccgtcgggat gatgataccg cgcatgatca
      901 cgttctggca aaggatgaag acgaacagcg tcggaatcgc tgccaggacc aatgccgcga
      961 acatcacgaa ggtcggcgcc cactgctgca tctgcgacag ccagaccatc agcgtccaca
     1021 tcttcgggtt ctggcagacg agcaacgccc acatgaacga gccgtaggca gaggtgaacg
     1081 tctgaagcgc aatcaccgcc atgaccggca gcgagagtgg gacggtgatc ttcgtgaaca
     1141 tcagcgtctc gccggccccg tcgatcgtcg ccgcctcata cagctcgctc ggcaggctgt
     1201 cgaagaagcc tttgagcagg aagatcgagt agccattcgc catggccggc aggatcagcg
     1261 cccagtaggt gttcagcagg tggaactgct tcagcaggag gaagcgcggg attgcactca
     1321 cctcggccgg gaacgacatc gtcgccagca ggaagagcag gatcttgtag gtctgtggca
     1381 gcttgaaccg cgacaggccg taggcgcaca gcggattgac gatcagcgtg accgacatga
     1441 ggccgagcac gaggatcatc gtgttccaca gcgaccgccc gtgcaggacg atatagttga
     1501 acacctcccg gtagttgcgc accaggaact cgcgcttcac cgccttgcgg tcggcctgca
     1561 tctcgaccca gtccgcttcc aggtagggag gcctcacctg ctggaacgac gcaagcgaca
     1621 caccccacgc cttgttcagc gcttccacgc ttccgtactt cgtcttcaga tgcgcgcggt
     1681 agaggatctc cggcgtctgc agatagatca tcttcgtggg cagaacacgc tcgacgaagg
     1741 cgatccagtc ggcctggggc atgccgctca ccggcgcctt gcgcggggcc ggcaggggaa
     1801 tctcgtggaa cccggcatac ttggttccgt agcgcgcgtt caggacctgg atgttcccct
     1861 cgtactgctg ggccaggaag cggcggtaga gcggctccgc ctccgggtcg gcgcccacga
     1921 actgggcggg cagctccttg cgaacgaact cctcccagtc ggcggcctgc ttggggtgcg
     1981 agggcacgcg ctcggaaagc gccacctcaa accggctctt gtagttcgtt ccccacgttt
     2041 cgttgagcac atccggctcc gtgccatagc gcaagcggag ccagtcttgg aagaggccat
     2101 cgccactcgc ggtgttgaag taacgttccg gctgctgagc gcggaactcg aggaactcct
     2161 tgtatttccg gttgatcacc ggctggtatt ggcgctggtt ccagtcctcg acggggatga
     2221 ggacctcttc ccacacctga ttgctctcgg tgtaggcgcg gttcacagcc tggagatcgc
     2281 ctttataccg ctcgcgcagc cacgcctgat agcgcgattg catctccggg gtgatgcgcg
     2341 agaggtttgt cgtgcccccc agcatcttgt aggtatcggg aagcgtcttc ttgaatgcca
     2401 gccaatcatc taccatctgc tggttgatgt tggccggaac ttctaccttc ttgaactcgg
     2461 ggatgtcgtt gctcaggtag gcgttgaact tcgggatatc ctcgttgtac ttcgcctcga
     2521 cgtacttgcg gaagagcatg gcgtcgtcgt agaagtacga cggcacgggt gcgaactcgt
     2581 ccgagtctac ctcgctcgtg aacgacgtgg agaccatgat caagaagggg tagaccatgg
     2641 tgatcgcgcc gagcgtgagc accgtataga gcatcgcgat gatgaggcgc atcttcggac
     2701 tgcgccgtcc aacagttgga atgatcggca tgacttcctc gcacaacccg ggaaggcatg
     2761 ccccgcgggc tctcccgcgg ggccgcctcc ccaccttcac tcacataggg cggatgatga
     2821 tcacgcctgt gccgtcttga actgcacccg gctgaggatg cgtagctgca ggacggtgaa
     2881 tccgaccagg agcgcgccta cgatccacgc catcgctaca gcgtagccga acttctggta
     2941 gatataggcg gtgtagaaga tctccagacc gataacccgc gtggtattca ccgggccgcc
     3001 acccgtcatg atgaagaccg cgtcgaacgc ctgcgcggcg gcgatgaacg cgcccacgaa
     3061 gttgatgatg atcagcggcc gcagataggg cacggtgatc cgccagatct ttgagagcag
     3121 tccggcgcca tccaggtcag ccgcttcata catctcctct gggatgctct tgagcgccgc
     3181 caggtagatg agacaccccg gccccatccc cgcccagacc agcgggatga tgatcatcat
     3241 catcgcccaa cgcgggtcgc ccagccagtc ttgcggagtg tagatgatcg ggttggcgcc
     3301 cacttgggtg atcacggcat tcaccgcggc aaccgcaccg gcgatcacct tgttcagcag
     3361 gccggaggga gtgcggtcgt agaactgctt ccagagcatg aagatgacca ggccggtggt
     3421 cacggccggg aggtagtaga gcgtccggaa gagcaccttg ccgcgcggca cctcattgag
     3481 cgccagcgcc agggcaatcg gggtgaggaa cccgaaagca agcgagtaga tcatgtagcg
     3541 ggcgctgttc ttcaggccga gccagaacgc cggcgcaaac agcacttccg cgaagttgtc
     3601 caagcccacc caggtggtgg gtagcaccag cttatagtcc tggaaggcca tcagcgatcc
     3661 gcgggcgacg ggatagtatt gccagacggc cacggagatg atggccggga gcatgacgat
     3721 ccaggcgtag ccgaagcggc gccagccctt gccggtcact ggcggcggct cgagctcctt
     3781 cgcgaaggtc cgcatcacgg tgcggaacac gaaagcaaag ccgatgatca gcccgaacac
     3841 gaccatgccg gcgaccaaac gccgcttctc cttgacgccg ggcggaagca gccccatcag
     3901 cttcacattc gtctcggcaa cggacttatc caggagcgcc ttcaggtact tcggatcggt
     3961 gttcgtggag atccacgcct gctcgatggg cggcgtcatc tccgtgtaaa tcatctcgca
     4021 gttcttgccg tagggctcgg ggcgcccgct gttcagcgcc tcttccaagg tttcggccca
     4081 cccgggcggc acctggcggc tgaagcggtg atactcctcg ggagtcaggt agcgcttcag
     4141 gtactccgga ttgaggtact gtgcgtagcc agcctccatg tagatccgcg tgcggatccg
     4201 cttcgcctca tcgcccgcgc ggaacttaat ccactcccag gcagcgcgcc ggacccgcgg
     4261 atccttgatg gtggcgttca ggccctgcat cgtggcgttg atctcgttgc cgcgcttccc
     4321 ggaagggccc atcggcatcg gaccgacgcc gatcaggttg gggttcacct gagacatcac
     4381 ctggtcgttc atgtactgga agtacatgcc cagcttcccg agcgtccact tcgtgtagac
     4441 gtcggtgccc cggtaggcga cacccttcgt cttcttgccg ttgcggatgt actcgccctg
     4501 gttgagcttc tggtagaatt ggagcgcctt caccgcttcc ggagtgttat acacggcacg
     4561 ccactggcca ttctcctcca caacggcttc gccgccggcg gaccagagga agttgatgaa
     4621 gtgccaagag gcctggggtc cggccgcgat cccgatgccg tactcacctt cctccgggtt
     4681 ggtcatcttg gcggcatact caaacagctc gtcccaatcc ttcgggggcc gatctggatc
     4741 gagaccagcc ttgcggaaga ggtccttgcg gtaagccagc gtctgcacca cagtgccgta
     4801 ggggatggcc cacacgtgct tctcgtgtcc cggaccctcg cgatagatga ccggccacac
     4861 ctggggcttg atgatgtgcg acagaaccgg ctgagcttcc tcgatggtcc tgatcggccg
     4921 gcctgccagc tcggttgccc actcgagcac gtactcatca agaggtgcca ggaagccctg
     4981 ggcgatgtag ttctccgaga tacggaagtt gacatagagc acatcggggg caacaccacc
     5041 cgccatggcc agaagcgggg tgatatcctg gcccatgccc ggcgcggtga tgccgcggaa
     5101 gccatccatc tcaatctcgg ggtgcagctc catgaagcgc tgcagcaccg cgaggtctgc
     5161 acgctgatcc accgtgcgcg cttcccggtt ggggagctgc cacactttca cggtgacagg
     5221 ggctgcctgt gccgcgctgg cgccgaacgc cagcacccac agtgctagaa agccaaacaa
     5281 ggagcgatat gccaggggca tgcgaaaacc tccgaattga aaagagggac ctgcagcacg
     5341 cacgcaccgg ggccgtatga tcgagcggac gtagggcgcg ggttcagttg ccaggtccga
     5401 cgcaacggtt gtatcttacc ttgggcggct ctctttgtca agacggcacg ccgatcaacg
     5461 gggttcctcg cggcgggttg atccggcagg caagctcgtc tgtgcagttc tcaaaggctc
     5521 cgtccatcac gctgccagca ggctcccacg gctctccggc tcccgttgca tgcagccaca
     5581 cgagcccgcg ggaaatcgcc aggcgaaaga cagcgtccga tatggcgtgt gttctgcaag
     5641 cgaggtccca cttccttcgg cacagaagca agaaggggca ggccgcgggc agacgaaaga
     5701 ggcagcatgg cggcgctggt tgagatcatg ggtgcgggaa ggatgccgct gttggggcac
     5761 ctcttgcatg ccctccatac ccggatcaac ctcggagggg ggtgggcatg agccgggcgc
     5821 ttccggcgct cttccggcgc ttcccgcagg cgcgcgagca cctcccctgg atcccactag
     5881 gtgatctgcc gacgcccctg gaatccctgg gaaatctgcg gggtcgcgcg gcgccggcgt
     5941 cggtctgggt caagcgagat gaccgctcat cccgcctcta tggtggcaac aaagtgcgcg
     6001 ccctggagtt tctcctcgct gatgccctct gccggcgtgt cgatcgggtg ctcaccgccg
     6061 gtgccatcgg ctcccacttc gtctccgcca cagcgctttt ttcacgccgg gtgggcttgc
     6121 gcgccgaagc catcctgtgg agccagccct ggagtgaacg ctgcaccgaa gctctcgatc
     6181 tgctccatcg cctggacgtg ccggtgcgtt cctatgccca tccagccatc gccgtggccg
     6241 cgctcatcct gtccggcgtc caacaccggc taagcggccg gcgcgtggcg ctcttcccgc
     6301 ctggtggctc ctcgccacgc ggcgcgctcg gcttcgtcga tgccgcgctc gaggtggcgg
     6361 aacaggtggc cgcgggcgag atccccccgc cagagtcgat ctgggtggcg tgcggttctg
     6421 gcggctccgc ggccggactc gcgcttggcc tctcgctggc gcagttgacc acgcgcgtcc
     6481 gcgccgtgcg cgtgacgccg cgccttgcct gcaaccgcca ccggctgttg gggctggcag
     6541 ccggggcgtg gcggcttctc tgccagggaa tggaagcgga aacggaaggc gcggccccag
     6601 gcgcgaccca cgcgggagag gcgcccgcgg gcggcccccg ttttggcgac cgtctggagc
     6661 tcgtggaggg cttctacccg cccggttacg cgcgcgaaac gcccgcatcc cgcgacgcgg
     6721 ccgaactcct cacacacatc ggactgccgg gcgataccac ctacaccggt cgggccatgg
     6781 ccgccctcct gcagcgagcg ccactcgagg gcggaaccca tctttactgg cacacgcttg
     6841 gcagcgaggg cgcacagggg tagtccagaa ggccacccaa ccaaaccctt tgcgccaggc
     6901 gcttgccagg agcgcgcctg ggtacttggc taccaggcca gccagaggca atcgttgaag
     6961 gtcgccgccg gcgtcgtgag cttctggatg gcataccact tcacatgccc atccatgaag
     7021 acgcagttcg ccccatccgc gtgccgcggg ttcgccgtgt cggtgtaaag cgtcccgctg
     7081 gtgggagtcg cgttcccgtc cggccaggcc caatcgtcat tcgcgtggcc ctgagctgcg
     7141 ttatccccgg cgagcggggg aaagcgcgtc cagggatcgg cggcgagcgc cctcgacttg
     7201 ccgttgtcca acgccatgat gcactgcgat gggacctgaa cctgggagag cgccatgttc
     7261 gcgttgaaga cacctgtgta gttgacgccg taggtattct gccggctgga cggacagacg
     7321 agcacgtccg tgttcttcag gtagggcgag gcgaccacga aagcaccaac gccggcctga
     7381 ttgtaaatag gaaacctctc gtcatagtcc tgagcatact gtaggaacgc cgttccaatc
     7441 tgcttcaggt tgctctggca cgtcgatttc cgggcgttct ctctcgcgcg cgcgaacacc
     7501 ggaaacagaa tggccgccag gatggcgata atcgcgatca ccaccagcaa ctcgatgagg
     7561 gtaaagcctc tggaatgcgg tctcgcggtg gagcccgtcg atatggagct tgagggaccc
     7621 atctgttctc ttcctttccg cttcctggag acgaaccgtg cctatgtggt ctcgtctatt
     7681 cgccttcgtg agacggactc ctgctaagat gccgctggtg atcttgcgcg atttgcgcac
     7741 gtgtgttgct ccaaccgcaa ccccgcccgt ttcggtccgc gggcagcttc cccgatcatg
     7801 ccatcgcgtg atgcgatctc agatccaggc gctacgcgct tccccaatcg cgcgcacctg
     7861 atgctcccgc aacagcccgt acggcgggaa tgtgaagcca tcggcgcccg catcacggca
     7921 ggcgtggagg cgctcccgaa gtgcttctcc cgtgccgatc tcttccgggg gcaccgcggc
     7981 aaggaggagc atgccgggcc gcagatagct gcgcgtatct tgcaggcgct ccggggcgtc
     8041 cgggtcgggc aacggcgccg tcgcgcggtc gctggcaaca agcacccgtg agggctcgag
     8101 gcctgtgagc gccatctcac ctggcagggg cagcgacgtg cgcacgtggc gcaccccctc
     8161 cccacgcgcc acggcggcga tctcctcgta gagcgaggtg gcgacatcgc tgcgcgcgtt
     8221 cagataggcc agca
//
LOCUS       DAOSCV010000056         8173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5708_length_8173_cov_4.121458, whole genome shotgun sequence.
ACCESSION   DAOSCV010000056 DAOSCV010000000
VERSION     DAOSCV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5708_length_8173_cov_4.121458"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1574
                     /locus_tag="PLY56_02445"
     CDS             <1..1574
                     /locus_tag="PLY56_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOJ20370.1"
                     /translation="GVLLDCGHNATVTSEHLRKWLRRLALLGFDTAMIYTEAGYSLPD
                     EPCFGYLRGAYTREELRDLDAYAAGLGIELVGAIQALGHLEQMLKWAPYAGVRDTEHT
                     LLVGEPRTYELVEKMVAHWASVFRSRRLHLGMDETYDLGRGRSLDLHGYRRGLDLYVE
                     HLGRVAEICRAHGVRPMIWSDVLFRLAGTSGAHYDRESRIPDAVRRALPADVDLAYWD
                     YYSDSPEHYEERIQKHRELGYEPVMASAVWSWPTPWHDWARTERNAGACIQACRSAGL
                     KEMIFALWSDDGGFWDVDSNLAGLAYAAEKCFGDGSVDEEALARRFRAICGADLAVHR
                     VAARINEPLQACSLLWDDPMLAIYLRHAAREGTEALREAAAHYEAVARDLEPHREDRA
                     AGDLGHAWRVARLFAAKTRLAARLLDAWAAGDRDALAAAREDAPGVAALVEELARSYR
                     GLWLARHQPFGLEVIQIRLAGQAERYRELARRIGEYLEGEVPTIPEFDEWRKGAYLPV
                     RSLSYRALATGSRVF"
     gene            2001..3386
                     /locus_tag="PLY56_02450"
     CDS             2001..3386
                     /locus_tag="PLY56_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOJ20371.1"
                     /translation="MRTQRLIRVNVRCLLLAMGVLVAVAGLDTRLASAPAEPGGKPVK
                     NIILMIGDGCGFPHLEAVSLYLHGEKGRLNVDQFPVKLGVRTYSFTGQGYDPAQAWAN
                     FEYVKKGATDSAAAATALATGIKTRNGYIAMDPDKVPVKTVLERAEELGRSTGVVTTV
                     QFSHATPAAFLAHNPSRNEYEAIAQEMLLRSKADVIMGCGHPWYRSDGQRREAPGDYK
                     YVGGEATWAALTAGKAGGDADGDGVDDPWTFIETRDAFRALATGDTPKRVCGVAQVSS
                     TLQLGRPGDRKAAPFEVPLTEGVPTLADMVRGALNVLDNDPDGFVVMIEGGSIDGAGH
                     GNLSGRLIEEGVDFLRAVDAVVEWVNRHSNWDETVLIITADHETGYLSGPGSGPESQP
                     MWKPLVGNGVNKLPGMEWYSTSHTNSLVPLYARGRAAEALTKRVVGKDPVRGDYIDNT
                     EVGKLIFELLQ"
     gene            3731..5029
                     /locus_tag="PLY56_02455"
     CDS             3731..5029
                     /locus_tag="PLY56_02455"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOJ20372.1"
                     /translation="METIVSLCKRRGFIFQSSEIYGGLGSTWDYGPLGVELKRNVKEA
                     WWNTVVRQRGDMVGLDAAILMHPRVWEASGHVAGFADPMVDCRKCKQRFRADHLESLE
                     KCPECGGDLTEARMFNLMFKTFMGPVEDEAAIVYLRPETAQGIFVNFENVRETMRKKL
                     PFGIAQIGKAFRNEITPGNFTFRTREFEQMEIEFFVKPGEDEEWHQRWIQDRLEWYRQ
                     YGIQPEKLRLREHACDELAHYAKACTDIEYEFPFGWSELEGIANRTDFDLRQHAEYSG
                     RDLTYFDDQTQERYFPYVIEPSAGADRATLAFLIDAYHEEQVRNEKRVVLRFHKALAP
                     IKAAVMPLKRNEPRIVAIAKELADNLRKCMSAMYDDTGSIGRLYRRQDEIGTVWCLTV
                     DFQSLEDQTVTVRDRDTMEQIRLPIAEVPARLREQLDNAC"
     gene            5371..>5830
                     /locus_tag="PLY56_02460"
     CDS             5371..>5830
                     /locus_tag="PLY56_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ20373.1"
                     /translation="MAGISVMVAGAAPAGATPRVRVDGKIVRGAPVVVIGGHAMVPVR
                     GVIQRIPGARVHWDAPHKRIIVTRGKQRVRLHLYSGYAHLENEAVPLDVPVVMRDNQA
                     LMPLRFVSEFLGAQVSYHPERRLVEIRSGKPGTQVAGYRSRSVARSVPGRI"
     assembly_gap    5831..5840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5841..6149
                     /locus_tag="PLY56_02465"
     CDS             <5841..6149
                     /locus_tag="PLY56_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20374.1"
                     /translation="IGGVTLVTLPTPGEHGSVERRAAEVTDRLTEATPALFERGEFNL
                     HRLWLGSAGGDPVICIGNQAVVRVTQQDAEALGVPQDQVARTWLAQMRDGLRRVYGRR
                     "
     gene            7131..7373
                     /locus_tag="PLY56_02470"
     CDS             7131..7373
                     /locus_tag="PLY56_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HOJ20375.1"
                     /translation="MRSFRPRTPRIRVGKGMVINLGKRGASMTTKLGKGMKITTGPRG
                     TTFTTTTRLGKGPSTTTQTRNGRTTTRFNLGRLRFW"
     gene            7503..7868
                     /locus_tag="PLY56_02475"
                     /pseudo
     CDS             7503..7868
                     /locus_tag="PLY56_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420363.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
BASE COUNT         1409 a         2483 c         2837 g         1434 t
ORIGIN      
        1 tgggagtgtt gctcgattgc gggcacaacg ccacggttac ctccgagcac ctgcgcaagt
       61 ggctccggcg cctggcgctc cttgggtttg acacggcgat gatctacacc gaggcaggct
      121 attccctccc agacgagccc tgcttcggct acctgcgggg agcctacacc cgcgaggagc
      181 tgcgcgacct ggatgcctac gccgccggcc tgggcatcga gctggtgggg gcgatccagg
      241 cactcggcca cctggagcag atgctcaaat gggcgcccta cgcgggcgtg cgcgataccg
      301 agcacactct cctggtgggc gagcccagga cgtacgagct ggtggagaag atggttgcgc
      361 attgggccag cgtcttccgc tcccggcggc tacacctggg gatggatgag acctatgacc
      421 tgggtcgcgg ccggtcgctc gacctgcacg gctaccggcg cggcctcgat ctctatgtgg
      481 agcatctggg ccgggttgcc gagatctgcc gcgcgcacgg cgtgcgcccg atgatctggt
      541 cggatgttct cttccgactg gcgggtacgt ccggcgcgca ctacgaccgg gagagccgca
      601 ttcccgacgc ggtgcgccgc gccctgcccg ccgatgtgga tctggcctac tgggattact
      661 acagcgattc cccggagcac tacgaggagc gcatccagaa gcaccgcgag ctgggctacg
      721 agccggtgat ggcttccgcc gtctggagct ggccgacgcc gtggcatgac tgggcgcgca
      781 ccgagcgcaa cgcgggcgcg tgcatccagg cgtgccgctc ggccggcctg aaggagatga
      841 tcttcgccct ctggtcggat gatggcggct tctgggatgt ggactccaac ctggcgggcc
      901 tggcctacgc ggcggagaaa tgcttcggcg atggcagcgt ggacgaggaa gcgctagccc
      961 ggcggtttcg ggcaatctgc ggggcggacc tggcggtgca tcgcgtggcc gcgcggatca
     1021 acgagccgtt gcaggcgtgc agtcttctct gggacgatcc gatgctggcg atctacctgc
     1081 gccatgccgc gcgcgaggga accgaggcgc tgcgcgaggc agcagcccac tatgaggccg
     1141 tggcgcgcga cctggaaccg caccgcgagg atcgggccgc cggcgatctg gggcacgcct
     1201 ggcgggtggc ccggctcttc gcggccaaga ccaggctggc ggcgcggctt ctggacgcat
     1261 gggccgcggg ggatcgcgac gcgctggcag ccgcgcgcga ggatgcgccc ggggtggccg
     1321 cgctggtgga ggaactggca cgctcctacc gcggcttgtg gctggcgcgc catcagccgt
     1381 ttggcctgga ggtgatccag attcgcctgg ccggccaggc ggagcgctac cgcgaattgg
     1441 cccgtcgaat tggggaatac ctggagggcg aggttccgac catccccgag tttgacgagt
     1501 ggcgcaaagg cgcctacctg ccggttcgat ccctctccta ccgggccctg gcgaccggtt
     1561 cccgcgtctt ctgaggacga cggaggggga ggtcggtcgt tctccaaccg gcatcgacag
     1621 gcggccggca gcgtggcgat gaatcaccgc gctgcttgtg agcgaagtcc tatcgggctg
     1681 ggcctggccg cacgcccggc caacccggag tggcggggtc tgggagggga agccccgaaa
     1741 gggctttgcc gggaagcagc gcggagcttc gagcttcgtg cgagggctgg cggatgcccc
     1801 cggatggtcc gcgcgacgac gggggcttgt actgcccatc cgtatccggc cccttcatcc
     1861 gcggtatcga ggccttcacg gtgcgcccgg cgccggagcc tggtatgctg atacgtgcgt
     1921 tgactccgtc ccgggggtgt agtaaactgg gagtggtgcc agtcaacccc accagcggca
     1981 ccagaggagg caatgtgcca atgcggacac agcgtttgat tcgggtcaat gtccggtgct
     2041 tgctcctggc aatgggcgtg cttgtggcag tggccgggct ggacacgcgc ctggcgagcg
     2101 caccggcgga gccaggtggc aagccggtca agaacatcat cctgatgatc ggcgatggat
     2161 gcggcttccc tcatctggag gcggtgagcc tctacctgca tggcgagaag ggccgcctga
     2221 acgtcgacca gttccccgtc aaactgggag tacgcaccta ctctttcacc gggcagggct
     2281 acgatcccgc gcaggcgtgg gctaatttcg agtatgtgaa aaagggtgcc accgattcgg
     2341 cggctgcggc cacggcgttg gccaccggca tcaaaacgcg gaacgggtat atcgccatgg
     2401 acccggacaa ggtgccggtg aagacggtgt tggagcgcgc ggaggagctc ggccggtcga
     2461 ccggcgtggt gaccacggtt caattcagtc acgcaacccc ggcggctttc ctggcgcaca
     2521 accccagccg caatgagtat gaagcgattg cccaggagat gctgttgcgg agcaaggccg
     2581 acgtgatcat gggctgtggc catccctggt atcggagtga tggccagcgc cgagaggccc
     2641 ccggcgacta taagtacgtg ggcggcgagg caacctgggc ggccttgacg gcgggcaagg
     2701 cgggcggcga tgccgatggc gatggcgtgg acgacccctg gaccttcatc gagacgcgcg
     2761 atgcgttccg ggcgctcgcg accggcgaca cgcccaagcg cgtgtgtggc gtggcccagg
     2821 tgagcagcac attgcagctg gggcgccctg gtgataggaa ggccgcgccg tttgaggttc
     2881 ccctcacgga aggtgtgccc acccttgctg acatggtgcg cggggcgctg aacgtgctgg
     2941 ataacgatcc cgacggcttc gtggtcatga tcgagggagg ctcgatcgac ggcgctggcc
     3001 acggcaacct gtctggacgc ctcatcgagg agggcgtcga cttcctgcgc gccgtagatg
     3061 ccgtggtgga gtgggtgaat cggcacagca actgggacga gacggtgctg atcatcaccg
     3121 ctgatcacga gaccggctac ctgtccgggc cgggctctgg cccagagtcg cagccgatgt
     3181 ggaagccgtt ggtaggcaat ggcgtcaata agctgccggg catggaatgg tatagcacga
     3241 gccacaccaa cagcctggtg cccctctacg cccgcggccg tgcggccgag gccctcacca
     3301 agcgcgtcgt tggcaaggac ccggtgcgcg gcgattacat cgataacacc gaggtcggca
     3361 agctgatctt cgagctgctg cagtaagcgg gaagcataga gcgaagccgc ggcggggctc
     3421 ccatcggagc cccgctggcg ctctgagtgg tggcccgctt cacccactcg atgattcagc
     3481 tagctcgccc gcctgggcga agagggcttc gtggcaatcg cccggcgtct ccccagcacg
     3541 cgcgttgccc cggacctggt tgccggccgg tccgggataa gagggggctg ccgggcgggt
     3601 tcatcccccg gaagctctcg ttgacgatcc ccctcggatc cggtatactg agagcgcaga
     3661 ttcctcgcgt agtgcctgcg tgtctgtata tattctgtgt tggggaggtc aaaggcagcg
     3721 catggtagat atggaaacca tcgtatcgct gtgtaagcgg cgagggttca tctttcagag
     3781 cagcgagatc tacggcgggc ttgggagcac ctgggactac gggccgctgg gcgtggagct
     3841 caagcgcaac gtcaaggagg cgtggtggaa caccgtggtg cgccagcgcg gcgatatggt
     3901 cggcctcgat gccgccatcc tgatgcaccc gcgcgtctgg gaagcctccg ggcacgtggc
     3961 cggctttgcc gacccgatgg tggactgccg gaagtgcaag cagcgcttcc gcgccgatca
     4021 cctggagagt ctggagaagt gcccggagtg cggcggcgac ctcacggaag cgcgcatgtt
     4081 caacctcatg ttcaagacgt tcatggggcc cgtcgaggat gaggcggcaa tcgtctacct
     4141 gcgccctgag accgcccaag gcatcttcgt gaacttcgag aacgtgcgcg agacgatgcg
     4201 caagaagctc cccttcggca tcgctcagat cggcaaggcg ttccgcaacg agatcacccc
     4261 tgggaacttc accttccgca cacgcgagtt cgaacagatg gagatcgagt tcttcgtcaa
     4321 gccgggcgag gacgaggagt ggcaccagcg ctggatccag gaccgcctgg agtggtaccg
     4381 gcagtatggg atccagcccg agaagctccg gctccgcgag cacgcgtgcg acgagctggc
     4441 ccactacgcc aaggcctgca ccgacattga atacgagttt ccctttggct ggtccgagtt
     4501 ggaggggatc gccaaccgga cggacttcga cctgcgccag catgccgagt actccgggcg
     4561 tgacctaacc tacttcgacg accagacgca agagcgctac ttcccctatg tcatcgagcc
     4621 ttccgccggt gccgaccgcg cgacgctggc gttcctgatc gacgcctacc acgaggagca
     4681 agtgcgcaac gagaagcgcg tcgttctccg cttccacaag gctttggcgc cgatcaaggc
     4741 cgcggtcatg ccgctgaagc gcaacgagcc gcgcatcgtg gccatcgcta aggagctggc
     4801 cgataacctg cgcaagtgca tgtctgccat gtacgacgac accggctcca ttggccgcct
     4861 ctaccggcgc caggatgaga tcggtacggt atggtgtctc accgtcgatt tccaatcgct
     4921 ggaagaccag acggtcaccg tgcgcgaccg cgatacgatg gagcagattc gcctgcccat
     4981 cgccgaggtg cccgcgcgct tgagggagca gctcgacaac gcctgctaac ccagggacgc
     5041 gggccaggcg ccgcgtcttc cggttacaga tgaggcgagg ctacggagac tacttctccg
     5101 tggcctcgct ttttttcggg gttgccgggc acgcgttccg ccgtggagtt ccccgctcct
     5161 cttctccccc ttggtttgat acccgtttgg cacagcaatt gctggaggca ccctgccggt
     5221 ttgtgacgac cctgccactg cgcgcggatt gcgccgccgt gatgcagagc gttgggcgcc
     5281 agctcacgcg gggccgtcgc gccggaacga cgcagattca agccggaggg aggaacctcg
     5341 agcatgcctt ggagcaaacg attcctgtgg atggcaggga tatccgtgat ggtggcagga
     5401 gcggcgccgg ccggcgctac gccacgggtg agggtggatg gcaagatcgt gcgtggtgcc
     5461 ccagtcgtgg tcattggtgg gcacgccatg gtgccagtac gcggcgtgat ccagcgtatc
     5521 ccaggggcgc gcgtccattg ggacgcgcca cacaagcgaa tcatcgtgac gcgtggcaag
     5581 cagcgggtgc gcctgcacct ctatagcggc tacgcccatc tcgaaaacga ggcggtgcca
     5641 ctggatgtgc ccgtggtgat gcgtgacaac caggcgttga tgcccctgcg tttcgtcagc
     5701 gagtttctcg gcgcgcaggt gagctaccat ccggagaggc gcttggtgga gatccgcagt
     5761 ggcaagccgg gcacgcaagt ggcgggctat cgcagtcggt ctgtggcgcg cagcgttcca
     5821 ggaaggattg nnnnnnnnnn atcgggggag tcaccctggt gacgctgccg actccgggcg
     5881 agcacggttc ggtggagcgg agagccgcgg aggtgaccga ccgcctgacg gaggccacgc
     5941 ccgccctgtt tgaacgcgga gagttcaacc tccatcgcct gtggctaggg agcgccggtg
     6001 gcgatccggt gatctgtatc ggcaatcagg cggtggtgcg ggtgacccaa caggacgccg
     6061 aggcgttggg agtgccgcag gatcaggtgg ctcgaacctg gctggctcag atgcgcgatg
     6121 gtctacgtcg cgtctatggc cggcgctgag atcgcgaagc ctcccccgga gggaagggag
     6181 aagcgcgggc atcggagcca gggtcctcca ggcccgcgcc ggcgtcaata ccacggatga
     6241 aggggacggg cgcgggtggg aaaggggctt tcctgccccc cgccccctcc ttttaagcag
     6301 tgggtcgccc gttgctattc gttgcctcat cttggttgtt accgaaatgc gctcccgcgc
     6361 ctgggcgata ggcactcttc cctgaccgtc attgtagtgt atcgtgggca gggcaaagct
     6421 tggggccatg ctggggaacc gcccgcgcgc tgcatagatg gcatgctcca aaagatgcgt
     6481 gcgccctgtc ctctttcagg atagaccgtc gcttcgctgc tcatactctg cgcgccggcg
     6541 gcgagcgatc tgtcgtaccg aaggcacggt catcctctct atgcttccat gccacggtaa
     6601 caggggcatt tgaggcaacg aatgcgttcg gtaacaccat gctcaggcaa cgaatgccac
     6661 ttcggtaacg tagatgttga ggcactgaga ttccgacata aggaatccga agcctcggag
     6721 gcagcgtgca tcccggcgcg cggcgataaa tcaccgcgct gcttgcgagc gaagccctgc
     6781 cgggctgggt tggggatact cccagatgtc tcaaaacggc ggtggctggg atgagaaggc
     6841 ccgaaggggc tttgccggaa agcagcgcgg tgatttatcg ccgcgcgtgc gggtggccct
     6901 ctccttatgt cggaatcgca ggttgaggca acggttcgtt gatctatcgc cgaggcgcgt
     6961 gctatagtct aatacggcag cttcgccgct tccgcggccg gcgtggaaga tgccagccct
     7021 ttgttacgct gtcgcgtaat cgctctgacg agtagagtcc tcgcttgggg acaagccacc
     7081 tgctatggca cacgccagca acaggtactc gggaaggaga ggatggagca atgcgttctt
     7141 tcaggccgcg cactccacgg atccgcgtag gaaagggaat ggtgatcaac ctcggcaagc
     7201 gtggggcgag catgactacg aagctcggca aagggatgaa gatcaccact ggtccgcgtg
     7261 gcaccacctt cacaaccacc acgcgactcg gcaaggggcc aagtactacc acgcagaccc
     7321 gcaacgggcg caccacaacg cgcttcaacc tgggccgcct gcggttctgg tgagcgatgg
     7381 gtgcccctgg ggggattgcc ggcccggggc atcgtttcgc atcggagagc ggggggtatc
     7441 tggcgaggga tacgcacctg cattcccgca gcctgcgcga catcagcgtg aggagagaga
     7501 cgatgccgag agtaatccac ttcgaccttc cggcggacga ccccgagcgg gccatccgct
     7561 tctacgagac agtgttcggc tggcagatcc ggaagtggga agggccgatg gagtaatggc
     7621 tgatttcgac cggccctgaa gatgagccgg gaattgatgg gggcctcgcg cgccgaatgg
     7681 caagagaggg cgtgagcaat actatcggcg ttccggacct ggaggagtac gtccggcggg
     7741 tggttgatgc cggcgggaag gtgttgatgc ccaagaccgc cattccgggc gtgggatggc
     7801 ttgccacctg cgaggacact gaaggtacgc agttcggcct gatgcaagcc gaccccaacg
     7861 cccagtaatg aagacagcat ccgcgggtga aaccgcccgc ggatgctgtg cccttcgtcc
     7921 cgcgcacccg gcgattttgt tctccttgac agttgatgca ggcttctgct atactgaccc
     7981 cggatagcga gtggctatcc tctttttttg cgtgcgctcc tgaccttggg atttgggggt
     8041 tcgtgatagg cgctccgctc ctacttccct cgaagtagac ccgggctgct ctttgcacct
     8101 cacgcttgga tgggtctggt ggcgtcggtc gtgagcgccg atgccgcagc ggcacgcgct
     8161 ttctgaagga gat
//
LOCUS       DAOSCV010000057         8102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5768_length_8102_cov_3.257487, whole genome shotgun sequence.
ACCESSION   DAOSCV010000057 DAOSCV010000000
VERSION     DAOSCV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_8102_cov_3.257487"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLY56_02480"
     CDS             <1..260
                     /locus_tag="PLY56_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20376.1"
                     /translation="VAGRSRFLDRYRNRIVAFRGNQVLWSARTVDEMIRIERTWCQGP
                     WDAPEFMVRVLPEEDEIECLEAYTEVLRAGREERVATGRVM"
     gene            338..2833
                     /locus_tag="PLY56_02485"
     CDS             338..2833
                     /locus_tag="PLY56_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20377.1"
                     /translation="MASAVRESFRKRVSRASLGGKVARVVVPKGWGRMVEMMGDTFRE
                     QMPEHLEGAVRDLLDEGEEPRITLSADLDRNARFGGSSWLIATNRRLLILSPMGKPSS
                     LAPAGESRKPKRRRWCLPGREALDVPLLPPPYHLTHVPLAKAVEVKARETVGGGVLQV
                     GFQAEEEGAPPVRVDLLRFSRTHLLRIGEAAQLIERMRQELAGQEKAGAAIGNRRKRS
                     RARCERCGRVLPPGSDTCPACVNKGKVLIRLLGYLGPYWKLSVLSLLASIGLTALALT
                     PPYLMRLLIDEVLTPRKNAHLLFVITGILAAVHLVRLAVGAGRDWLNSWLGQRVYFDL
                     RTQIYRHVQRLSMGFYDRKQVGGVLSRITSDAGTLHYFLVGSAQNLLIHTLTVAGIAV
                     MLFVMNWRLALIVLMPTPLLIVGTFWFGRRVRVVYRRYWRLISSINSLLVSTISGARV
                     VKSFGQESREVGRFEDRSDEFRNTSLAAARLSVIYYPSMALITVISTILIWLVGGQQV
                     LAGTLSLGMLTAFTSYMWQFYAPISVLCEINNVLQQTATAAERVFEVLDTEPDVAESP
                     NAITLDRIEGHIEFRDVCFSYDEAEALEPLLKNINLVVQPGEMIGLVGASGSGKTTLV
                     NLLLRFYDPTSGQILIDGHDLRDVKLRCLREQMAVVLQEPYLFKGTIAENIAYGRPDA
                     TREQIIEAARAANAHDFIIKFPDGYDTEVGERGGRLSGGERQRVSIARALLNDPRILI
                     LDEATASVDTETEKQIQEALEQLMKGRTCFAIAHRLSTLRMATRLVVLDGGRIAEIGT
                     HEELIAKDGIYAKLVKIQSEMAKSIAPPGEVGR"
     gene            2845..3315
                     /locus_tag="PLY56_02490"
     CDS             2845..3315
                     /locus_tag="PLY56_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOJ20378.1"
                     /translation="MRDEGTIRLAYDVMGRFLLHRGAEPPIPVRPVRLFPLSDPEGYI
                     LLLDHEEKEVTLIRNMKELDPASRQALRQELETAYIVPEVLCIESIEEEFGVLHFVAH
                     TDRGPRRFDVRGRGEIQFVRPGYYIIRDIDGNRFDIPNLFALDARSLALLDPYL"
     gene            3631..4182
                     /locus_tag="PLY56_02495"
     CDS             3631..4182
                     /locus_tag="PLY56_02495"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ20379.1"
                     /translation="MSNLLLIGARASGKTAVGRRAATLLGEGWGFLEMDEALVERLGM
                     PIAEFFRVHGEEAFRREERALLEEVGRGQRRVVACGGGVAGTAESLALARATGTLVWL
                     DTPVPELIARRLADPDEAARPALLHELAELKRQDLEGYLRVEVPTILARRQAFYAGAD
                     FTLYTAGLSVERLAQIVAALARR"
     gene            complement(4396..5367)
                     /locus_tag="PLY56_02500"
     CDS             complement(4396..5367)
                     /locus_tag="PLY56_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ20380.1"
                     /translation="MRILVTGGAGFIGSHIAERLLADGHQVVVLDNESTGFAANVPTG
                     ACYIRGDVRSPEDVERAFTALGGLDVVYHIAGQASTIKSFDDPGTDLDVNTRGTLNIL
                     QGCIRHRVTRLLYASSMVCYGHVTQVPTPETHPCRPISYYGITKYAAERYVFATAERP
                     DLDFPLNVTAFRMFNVYGERQSLSNYYQGVVTIFMANVLKGEPITIHSDGEQSRDFVY
                     ISDVVDAWVGALENPRSYGEVMNLGTGTRKSINQLVDVTLEALGHSRADWEIRYTPVR
                     PGDQRHMEADISRAREILGWTPQVSFEEGMARTIRWAVSEHRRAQAK"
     gene            complement(5364..6119)
                     /locus_tag="PLY56_02505"
     CDS             complement(5364..6119)
                     /locus_tag="PLY56_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ20381.1"
                     /translation="MLLSVVVPAHNEEECIEKVLLDLVRALDDAGIPHEIVAVDDNST
                     DRTGEILDELAAREPTIRPLHRTSQPGFGRAIRAGLAAMRGDCCAIVMGDGSDDPADV
                     VTYYRKLEEGYDCVYGSRFIRSGRVVDYPRFKLLVNRLGNTFMRILFWTHHNDLSNAF
                     KAYRTEVIRAIEPIQASHFNITVELSLKPLVRGYRIATVPISWYGRTAGVTKLRLKVM
                     MRKYLFTILYVWLEKHLIADEYVAPRKEEARVP"
     gene            complement(6201..6890)
                     /locus_tag="PLY56_02510"
     CDS             complement(6201..6890)
                     /locus_tag="PLY56_02510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20382.1"
                     /translation="MKSGERPETFLSGEESARLYQVRFDESARRRNAAIWCVLCRRFF
                     QRYVPEDATVVDLGAGHCEFINHIRAARRIAVDTNPETRECAAPGVEVVACDIRTLEG
                     VPPATADVAFASNVLEHLPSKADLMATLGAVRRVLRPGGRLLVMGPNIRFAPGEYWDF
                     LDHHIALSDRSVREAVELAGFTTREIRPRFLPLTRLSRIPQHPLLVELYLRFPPAHRV
                     LGKQFFLAAER"
     gene            complement(7291..>8102)
                     /locus_tag="PLY56_02515"
     CDS             complement(7291..>8102)
                     /locus_tag="PLY56_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ20383.1"
                     /translation="RAKNMGAEIVAFPEIYPQIAAPDHYAAAEPHDGGTLPAIQALCK
                     ELDLYVIWPRFESRPSGLYNTAILIGRDGEVIGRYDKMFPTLGEMEKGVRPGTSCPRF
                     ETDFGRVSMAICFDLNFIEIRDELRADPPDVLFFCSLYRGGVQLQEWAIDLGCHVMSA
                     IGTELGRLVDPGGQVLKLSTYETLLAARVNTNKRQLHMDFNWHKMDDMLARYGSKLTF
                     EYYTPEARFVIGYQGTDRDVDEILAEFDLLPIQEYWARVRRTRAEKLTQAS"
BASE COUNT         1335 a         2548 c         2766 g         1453 t
ORIGIN      
        1 acgtggccgg ccgatcccgc ttcctggatc gctatcgaaa ccgtatcgtg gcgtttcgcg
       61 ggaaccaagt gctctggtcg gcgcgcacgg tggatgagat gatccggatc gagcgcacct
      121 ggtgccaggg gccgtgggac gcgccggagt tcatggtgcg cgttctcccc gaagaggatg
      181 agattgagtg cctggaggca tacacggagg tactgcgggc tggccgcgaa gagagggtgg
      241 cgaccgggcg agtgatgtga ggggacggat cctgcgaagg caggagggag gccggatgcc
      301 cggctgaggt gtctgcggct gcatggagca ggcgggcgtg gcatcggccg tgagagagag
      361 cttccgaaag cgggtttccc gggcgagcct cgggggaaaa gtggctcgtg tggtggtccc
      421 caagggatgg gggcggatgg tggagatgat gggggatacc ttcagggagc agatgccgga
      481 acacctggag ggtgccgtgc gggaccttct ggatgaaggg gaggaaccac ggatcactct
      541 cagcgccgac cttgaccgga atgcccgatt cggcgggtcg tcctggctga ttgctacgaa
      601 tcgccgcctg ctcatcctca gcccgatggg gaagccctct tcactcgcgc ccgctggcga
      661 gtcgcgcaag ccgaagcgaa ggcgttggtg tctgccaggc agagaggccc ttgatgtgcc
      721 gctgctccca ccaccctacc acttgaccca tgttccgctg gcgaaagccg ttgaggtgaa
      781 ggcgcgcgag acggtggggg gcggcgtctt gcaggtgggg ttccaggcag aggaagaggg
      841 agccccgccg gtgcgcgtcg atctgctgcg cttctcgcgc actcatctgc tgcgcattgg
      901 cgaagccgcg cagttgattg agaggatgcg ccaggagctg gccggccagg agaaggcggg
      961 agcggccatc gggaataggc gaaaacggag ccgtgcgcgc tgcgagcgat gtgggcgtgt
     1021 gctgcctcca gggtcggata cctgcccggc gtgcgtgaac aaagggaagg tgctcatccg
     1081 tctcctgggc tatctggggc cctactggaa gctctcggtg ctctcactgc tggccagtat
     1141 tggcttgacg gctctggcgc tcacgccgcc ctacctgatg cgtctcctga tcgatgaggt
     1201 gctgacgccg cgcaagaacg cccaccttct cttcgtaatc acggggatcc tggcggcggt
     1261 gcacctggtc cgcctggccg ttggtgccgg acgcgactgg ctgaactcct ggctggggca
     1321 gcgggtttat ttcgatctgc gtactcagat ttaccggcat gtgcagcggc tctcgatggg
     1381 cttctacgac cgcaagcagg taggtggggt gctatcgcgc atcaccagtg atgccggcac
     1441 gctccactac ttcctcgttg gcagcgctca gaacctgctg atccacacgc tcaccgtcgc
     1501 ggggatcgca gtgatgcttt tcgtgatgaa ctggcgcctg gcgctgatcg tgctgatgcc
     1561 gacgccgctc ttgatcgtgg gtacgttctg gtttggccgg cgcgtgcggg tggtctatcg
     1621 ccgttactgg cggctgatct ccagcatcaa ctcgctgctc gtctccacga tctccggggc
     1681 gcgcgtggtg aagtcgttcg ggcaggagag ccgcgaggtg gggcgtttcg aggaccgcag
     1741 cgatgagttc cgcaacacgt cgctcgccgc cgcccggctc agtgtcatct actacccctc
     1801 catggcgctg atcacggtga tcagcaccat cctgatctgg ctcgtcggcg ggcagcaggt
     1861 gctcgcgggc acgcttagcc tggggatgct gacggcgttc acctcctaca tgtggcagtt
     1921 ctacgcgccg atctccgtgt tgtgcgagat caataacgtg ctgcagcaaa ccgccaccgc
     1981 cgccgagcgc gtctttgaag tcctggacac cgagccggac gtggcagaat cgcccaacgc
     2041 aatcacgctc gaccggatcg aggggcatat cgagtttcgg gatgtctgct tctcctatga
     2101 cgaagcggag gcgctggagc cgctgctcaa gaacatcaac ctggtcgtcc agccgggcga
     2161 gatgatcggc ctggtgggtg cgagcggctc gggaaagacg acgctggtca acctgctgct
     2221 tcggttctac gacccgacct cggggcagat cctgatcgac gggcatgacc tgcgcgacgt
     2281 gaagctccgc tgcctgcgcg agcagatggc ggtggtgctc caggaaccgt atctcttcaa
     2341 gggcacgatt gccgagaata tcgcctatgg ccggcccgac gcgacgcgcg agcagatcat
     2401 cgaggcggcg cgtgccgcca atgcccatga cttcatcatc aagttcccgg atggctacga
     2461 caccgaggtg ggcgagcgcg gaggtcgtct ctccggcggc gagcggcagc gcgtctccat
     2521 tgcgcgcgcc ctgctgaatg acccgcgcat cctgatcctc gacgaggcga ccgcgtccgt
     2581 ggataccgag acggagaagc agattcaaga agccctcgaa cagctaatga aagggcggac
     2641 ctgcttcgcg attgcgcatc gcctctccac gctgcgcatg gcgacgcgcc tggtcgttct
     2701 ggatggcggc aggatcgcgg agatcggcac ccacgaggag ttgatcgcca aagatggcat
     2761 ctacgccaag ctcgtcaaga tccagtcgga gatggcgaaa tcgattgcgc cgccaggaga
     2821 ggtggggcgt tgaggagcac tcagatgcgc gatgagggca cgatccggct tgcgtacgac
     2881 gtgatgggtc gtttcctgct gcaccgcggc gcggagccgc ccattccggt gcggcccgtt
     2941 cgcctcttcc ctctctcgga ccccgaaggc tacatccttc tcctcgacca cgaggagaag
     3001 gaagtgacgc tcatccgcaa catgaaggag ctcgatcccg cctcacggca ggcgcttcga
     3061 caggagcttg agaccgccta catcgtccca gaggtgttgt gcatcgagag catcgaagag
     3121 gagttcggcg tgcttcactt cgtggcccac acggatcggg gcccccgccg cttcgacgtg
     3181 cgcggccgcg gcgagatcca gttcgtgcgc ccgggatact acatcatccg cgacatcgac
     3241 gggaaccgct tcgatattcc caacctcttc gcgctggacg cccgcagcct ggccctgctc
     3301 gacccgtacc tgtagcgggg tccgcaggca tcgcgccgcc ttcgatacca cggatgggat
     3361 ggacgcgcgc acgcgcggag ctcgaagctc cgcgctgctt cccggcaaag cccctccggg
     3421 gctccccctc ttggctcccg ccacttccag ttgcccggaa atgtggtccg gcccagcccg
     3481 gcagggcttc gctcacgagc agcgcggtga tttgtcgtcg cgcgccgggt tgcgtgttac
     3541 cttcgaggtt tcggttccct atctcggaat cccggtccgg ccaggatccg ggggggcctc
     3601 cggcgtatca agccttggag agagtcgggc atgagcaatc ttttgttgat aggcgcgcgt
     3661 gcttcgggca agacggccgt gggaaggcgc gcggcgacgc tgttaggcga gggctggggc
     3721 ttcctcgaga tggacgaggc gctggtggag cgtctgggca tgccgattgc cgagttcttc
     3781 cgcgtccacg gagaggaggc gttccggcgt gaggagcgtg ccctgctgga ggaggtgggc
     3841 cgcgggcagc gccgcgtggt cgcctgcggt gggggcgtgg ccggcactgc cgagagcctg
     3901 gccctggcac gcgccacagg caccctcgtc tggctcgata cccccgtgcc ggagctgatc
     3961 gcgcgccgcc tggccgaccc ggatgaggcc gcacgcccgg ccctgctgca cgaactcgcc
     4021 gagctgaagc gccaagattt ggagggctat ttgcgcgtgg aggtgccgac aatcctggcg
     4081 cgccggcagg cgttctacgc cggggctgac ttcacgctct acacggccgg gctgagcgtc
     4141 gagcgcctgg cgcagatcgt cgccgcgctc gcgcgccgct aacccgcatt attcgtcaga
     4201 ataatgaaaa gaagtctctc cttgctgggc gtggcacggc ttcgcgggca ctgcccccga
     4261 aaacactcta cctccggacg ctcgtggcgg caacggcgtg ctaccaggca agagtcttct
     4321 tttgaagtat tctggtgaat acttcgggct aaagggcatg ggccgccacg tccggcaaac
     4381 ccggcgcggg atggctcatt tggcctgggc gcggcggtgt tcagagacgg cccagcggat
     4441 cgtgcgcgcc atcccctcct cgaagcttac ctggggcgtc cagccgagta tctctcgcgc
     4501 gcgggagata tcggcctcca tgtgtcgttg gtcacccggg cgcaccgggg tgtagcggat
     4561 ctcccaatcg gcccggctgt ggcccagcgc ctccaaggtc acatccacca gctggttgat
     4621 gctcttgcgc gtgcccgtgc ccaggttcat cacctcgccg taggaccgcg ggttctccag
     4681 ggcgcccacc caggcatcca ccacgtcgct gatatagacg aaatcccggc tctgctcgcc
     4741 atcgctgtgg atcgtgatcg gctcgccttt cagcacgttc gccatgaaga tcgtcaccac
     4801 gccctgatag tagttggaaa ggctctggcg ctcgccatac acgttgaaca tccggaaggc
     4861 ggtgacgttc agcgggaagt cgaggtccgg gcgctcagcg gtggcgaaga cgtagcgctc
     4921 cgcggcatac ttggtgatgc cgtagtagga gatcggccga caggggtgcg tctccggcgt
     4981 gggcacctgg gtgacgtggc cgtagcagac catgctgctg gcataaagga gccgggtcac
     5041 gcggtggcgg atgcacccct ggagaatatt cagggtgccg cgggtgttca catcgagatc
     5101 ggtgcccgga tcatcaaacg acttgatcgt ggacgcctgg cccgcgatgt ggtagacgac
     5161 atcgaggccg cccaaagccg tgaaggcgcg ctccacatcc tcgggcgagc ggacgtcgcc
     5221 acggatgtag caggcgccag tcggcacgtt tgccgcgaag ccggtgctct cgttgtcgag
     5281 cactaccacc tggtggccgt cggccagaag ccgctcggcg atatggctgc cgatgaaccc
     5341 ggcgcctcca gtcacgagaa ttctcaaggg actcttgcct cctctttgcg cggcgcgacg
     5401 tactcgtcgg cgatcaggtg cttctccagc cagacgtaga ggatggtgaa gaggtatttg
     5461 cgcatcatca ccttcaggcg cagcttggtg acgccggcgg tgcgcccata ccaggagatt
     5521 ggcaccgtgg cgatccggta gccgcgcacc agcggcttga gcgagagctc gacggtgatg
     5581 ttgaagtggc tcgcctggat cggctcgatg gcgcggatca cctcggtgcg gtaggctttg
     5641 aacgcgttgg agagatcgtt gtggtgcgtc cagaagagga tgcgcatgaa ggtgttcccc
     5701 agccggttta ccaggagctt gaaccggggg taatcgacca cgcgcccgct gcggatgaag
     5761 cgcgagccgt agacgcaatc atacccctcc tccagcttgc ggtagtaggt gaccacgtct
     5821 gccgggtcgt ccgagccatc gcccatgacg atggcgcagc agtcgccgcg catggcggcc
     5881 aggccggcac gaattgcccg gccaaagcca ggctggctgg tgcgatgcag cgggcgaatc
     5941 gtcggctcgc gcgcggcgag ctcgtcgagg atctcgccgg tgcgatcggt gctgttgtcg
     6001 tcgacggcga cgatctcgtg ggggatcccg gcgtcatcga gagcgcgcac cagatcgagt
     6061 aacaccttct cgatgcactc ctcttcgttg tgggcgggta ccacgaccga taagagcatc
     6121 ctctgttttc ctgtttcgtc acgcgcctcg ccgggccgat tctgtcgccg gacgccgggg
     6181 tcagccgcgt ccaggcgcgc tcatcgctcc gcggccagga agaactgctt cccaagcacg
     6241 cggtgtgcgg gcgggaagcg cagatagagt tcgacgagca gcgggtgctg ggggatgcgg
     6301 ctgaggcgcg tgagtggcag aaaccgcggt cggatctcgc gcgtcgtgaa gccggccagc
     6361 tccaccgcct cgcgcacgct ccggtcggag agtgcgatgt ggtggtccag aaagtcccaa
     6421 tactcccccg gggcgaaacg gatgttgggc cccatcacca ggagcctgcc gccagggcgc
     6481 aacaccctgc ggaccgcgcc cagcgtcgcc atcaggtcgg ccttcgacgg caggtgttcc
     6541 aacacgttgc tggcaaaggc gacgtcggcg gtggccgggg gcacgccttc cagcgtccgg
     6601 atatcgcacg cgaccacctc cacgcccggc gccgcgcact ccctggtctc cgggttggtg
     6661 tctaccgcga tccgccgcgc cgcccggatg tggttgatga actcgcagtg gccggcgccc
     6721 aggtctacca ccgtggcgtc ttccggcaca tagcgctgga agaaccggcg gcaaaggacg
     6781 caccagatgg cggcgttgcg ccggcgcgcg ctctcgtcga agcgcacctg atagaggcgg
     6841 gcgctctcct cgccactcag gaatgtctcc ggtctctccc cactcttcac ggctctgcgc
     6901 ggccctccac cagcattcta ccatacaatg cgcccgaaga gaaccgcctt gcgcttgggt
     6961 tccgagacag ggaaggcccg gacgcttgct tgcggtctcc tgcccagacg ccaccttcga
     7021 taccacggag gaaggggacg gacacggctg gagagcgccg gccacatgta cgggcatgaa
     7081 catgcacgcc gcgcgatccg cgaagggccg gtgtatcccc gcgcgcggag ctcgaagctc
     7141 cgcgctgctt ctctgcaaag cccttcgggc ttcccctccc ggctcttgcc attctgaggt
     7201 gcccggaagt gttgcccaac ccagcccggc agggcttcgc acgcaagcag cgcggtgctt
     7261 gagcgccgcg cgatcttgcc gggcaagtgg ttatgacgcc tgggtcagct tctcggcgcg
     7321 cgtccggcgc acgcgcgccc aatactcctg gatcgggagc aggtcaaact cggcgaggat
     7381 ctcgtcgacg tctcggtcgg tgccctggta gccgatgacg aagcgcgcct ccggcgtgta
     7441 gtattcgaag gtgagcttcg agccataccg ggcgagcatg tcatccatct tgtgccagtt
     7501 gaagtccatg tgcagctggc gcttgttggt gttgacgcgg gcggcgagga gcgtctcgta
     7561 ggtgctcagc ttcagtacct ggccgccggg gtccaccagc cggcctagct cggtgccgat
     7621 ggcgctcatc acgtggcagc ccaggtcgat cgcccactcc tgaagctgga cgccgccccg
     7681 atagagcgag cagaagaaga ggacgtccgg cgggtcggcg cgcagctcgt cgcggatttc
     7741 gatgaagttg aggtcaaagc agatcgccat ggagacgcgc ccgaagtcgg tctcgaagcg
     7801 cgggcacgac gtgccgggcc ggactccctt ctccatctcg ccgagcgtgg ggaacatctt
     7861 gtcgtagcgc ccgatcacct cgccgtcgcg cccgatcagg atcgcggtgt tgtaaagccc
     7921 ggacgggcgc gattcaaagc ggggccagat cacgtagaga tccagctcct tgcaaagcgc
     7981 ctggatcgcc ggaagagtgc cgccatcgtg cggctcggcc gccgcgtagt ggtcgggagc
     8041 ggcgatctgc gggtaaatct cggggaaggc gacgatctct gcgcccatgt ttttcgcccg
     8101 gc
//
LOCUS       DAOSCV010000058         8093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5775_length_8093_cov_4.329684, whole genome shotgun sequence.
ACCESSION   DAOSCV010000058 DAOSCV010000000
VERSION     DAOSCV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5775_length_8093_cov_4.329684"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..778
                     /gene="fliN"
                     /locus_tag="PLY56_02520"
     CDS             104..778
                     /gene="fliN"
                     /locus_tag="PLY56_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOJ20384.1"
                     /translation="MTDQPLSQEMIDALLQQAEGAGSAPAESSDKAAPPQPGDATPVA
                     EAAAREPSADTPNEAAAAPPPEAGAKEGAVPSGSPSASPAPAAAPAVPQDVPGFQISA
                     PSPGPLGSAQGGTDSTAGPTVRPALFSPLPASPPQAGGSSIDLLLDVPLQVTVELGRT
                     QLSVREVLGLGTGSVVELEKLAGEPVDLLINGRLIARGEVVVIDESFGVRVTEIVRSG
                     SATLHR"
     gene            799..1797
                     /locus_tag="PLY56_02525"
     CDS             799..1797
                     /locus_tag="PLY56_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20385.1"
                     /translation="MVAEVSEQTRSRPVSILVVDDDAMVLQVMAAGLRQSGYAVTTTT
                     SPLDGLRILRENVCDLVISDAYMPEMDGLTLAETANGIFPELPIVMLTGFGTVDLMRE
                     GLRRGASDFITKPFRMRDIPIIVERNLERKRLEKDRLSERSGRILFQAITSLLTAVDA
                     KEHCTAQHSRRVADISLQIGAALQQPEAEMRVLELAAWMHDVGKIGTPDAILRKPGGL
                     TDDEWQVMREHAAKGGEIIAQIEDLRYLASVIRHHHERYDGTGYPDRLRGDAIPLLSR
                     IIAVADAFETITSDRGYRSSRPVSVAVDELKRHSGTQFDPVVVETLLRLLESGRVL"
     gene            1925..2701
                     /locus_tag="PLY56_02530"
     CDS             1925..2701
                     /locus_tag="PLY56_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ20386.1"
                     /translation="MESDHLWRLYKETGDLAAREELILRHAFLVRCVADRLGFTGSAG
                     LDRDDVISYGIIGLIDAVEKYGACPSPSFESYARLHIREAILNAIRARRGQPRSLHAS
                     ADAIEQAVSDLQQRLGRHPTMEEVSAATGLSEEEIERVLICLESDGTALSESGDLPEG
                     ASDNLTLMPPDNGDDAPSPADPGRTARAEQRRILIEAIRRLPPEERLLLSLYYIEELT
                     LPEIGQVMNLPEAQVSQIHTKAMMRLRAHLRTQADLFAAA"
     gene            2712..2954
                     /locus_tag="PLY56_02535"
     CDS             2712..2954
                     /locus_tag="PLY56_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HOJ20387.1"
                     /translation="MIHLTRLDSAPIVVNCDLVESIEATPDTLLSLTTGKKILVKESV
                     AEVVEKVVAFKRLIAAGPRTDGAMNAARDEPARGTP"
     gene            complement(2999..3454)
                     /locus_tag="PLY56_02540"
     CDS             complement(2999..3454)
                     /locus_tag="PLY56_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOJ20388.1"
                     /translation="MGEQGGRVVALRMVVRPARLRVVLDTNLFVAAYWNPRSAPARVL
                     SACEENQLQLLYSAAIRREMDHILRKAHTSEAFRHRVSLLLEDAEEVCPTRTLHLVQD
                     DPEDDKFLECALEGDADYLISSDAHLLRLGPIDGACIIKPGAFARAHLM"
     gene            3682..6732
                     /locus_tag="PLY56_02545"
     CDS             3682..6732
                     /locus_tag="PLY56_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20389.1"
                     /translation="MRHWHFPVVILCLLLLPAADAAAATSLTPATLSALVHAKNEPGA
                     ASSAALHLRGNNTLVLLPVAGAQAGVALRAHAGASASQSVRYRVVRANGHEIHAGACA
                     PGKEVAWTIPAGVRCLLQIEAGQTPATLEVRNAGIGTHAARNAPAHLDRGAGQQCFLV
                     PKGTRSFTLTARAEGGAGKAVVRDPSGQPAASATLSSGEDALLKVTVDAKRSDRFWSV
                     SAEPAGEKGFTSVRLAFSGEIPPFLAPDPSGLALPFIHYPAVGALLMSDGNRSATIEA
                     VIAIEPQADRFVRAQMRRAGASRAMWTTRPSPFTTGRVRILLPDRLASGAYELKTTLQ
                     DQQGAIVATHAQPIHAASGFAHVGKERPLLELICQPQSDDSGASYQLRARVGIDPTCY
                     SQVRIQRALYREGESQPAFKEELGHPTGEVLPLKTPAGVSGGLYRMALNLVSQEGKPL
                     ATTSRSFYLEGNRCFMEVPPPAQAKKAPLNKSQRTRGYVLFSRVYHEAFPYNYEPPWE
                     DIGRPLYVWATPGEDEPATFGVYTLKPLQKARVKVGTLSRVGGGGVINPSSIEVRLAR
                     FWPQRTSDHSATYRLIPELLEPVTTATIPTGRISQYWLTLHIPPGTPAGDYRGMITYQ
                     PENAPASQLKLLVKVLPFRLERPASRTWGLYADSTRWRGATDATIEREMRDMKAHGID
                     SLALSALAHGTLTHAGEKLTVDFTPLSRLMGIYKRVGLRGPVILDLRDLPQVIAQAGG
                     ASTKPDDPVVQRLAGIVLSELAMRAETEKWPQAVYQLADELNAHSASSLATARALLSL
                     GKTASLPTLAFLASPQDGMRELDALIDLPCYGAEAVAGDKDGALRKALKEANKPLWWH
                     GSGCTSPRLEGNLTANRYLTGIAFWRSGAAAHWIQTYQRPMGNAYSDFDGDGQMTGKD
                     LCLTYPAPAGGAAVPTIQWEGIREGYDDYRYLYTFQQMVARAKRSGHERGAKYAAQVE
                     KEAQERLERLPWGAEPEGLTAYEAHAFRKWLSKYMGTLVNKLNS"
     gene            complement(6982..7851)
                     /gene="rfbD"
                     /locus_tag="PLY56_02550"
     CDS             complement(6982..7851)
                     /gene="rfbD"
                     /locus_tag="PLY56_02550"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008336196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOJ20390.1"
                     /translation="MRVLVTGAAGMLGRDLVEILSEQHDVIGAGRRGPDHCVDITDAR
                     AVHALLEAVRPDAVVHCAAYTNVDGCERDPDGAYRVNALGTAAVAGACHAVGAALVAI
                     STDFVFDGEKGRPYTEFDAPNPLSIYGASKLAGEELVRSLCPRHYIVRTQWLYGEHGK
                     NFPLAILQRADAGEELRVVSDQVGVPTYTRDLSRAIARILEQPLYGTYHAANRGEVSW
                     HGFAARLLEMAGLDPGIVRPISSDEWPSPTRRPAYSVLRPYVLELTGRAEVLRPWEEA
                     LAEFLERIGRLRA"
BASE COUNT         1445 a         2716 c         2633 g         1299 t
ORIGIN      
        1 tgccccgtga aagcgcctat ccaggaaggg cttcccggat cagggattgc gtctgttcgg
       61 ctatcacgcc ggatggactc tcaggtagag gagcttgggt acgatgacgg atcagcctct
      121 gagccaggag atgatcgacg cgctgctaca gcaggcagag ggcgccggat cggcgcctgc
      181 cgagagttca gacaaggcgg cgccaccaca gccgggggat gcgacccccg ttgcggaggc
      241 cgctgccagg gaaccaagcg ccgatacccc caacgaggcc gccgccgcac cgcccccaga
      301 ggcaggcgcc aaagaggggg ccgtcccaag cgggagccca tctgcgtcgc ctgcgcccgc
      361 tgcagcgcca gccgtccccc aggatgtgcc tgggttccag atcagcgcgc cgagcccagg
      421 cccgctgggt agcgcgcagg gcggcactga tagcacggcg gggcctaccg tgcgccccgc
      481 gctcttctct cccctcccgg cctcgccccc ccaggctggc ggctcgtcca tcgatctgct
      541 gctggatgtg ccccttcagg tgaccgttga gctgggacgg actcaactct ccgtgcgcga
      601 agtgctgggg cttggcaccg gttcggtcgt ggagcttgag aagctggcgg gagaacccgt
      661 cgacctgctg atcaacggcc gtctgatcgc ccgtggcgag gtggtggtga tcgacgaaag
      721 cttcggggtg cgcgtcacgg agatcgtgcg ttcgggcagc gcaacgctgc atcgatagga
      781 gttcgaggag aggagagtat ggtcgccgag gtatctgagc aaacgcgttc gcggcctgta
      841 tccatcctgg tggtggatga cgatgccatg gtgctccagg tgatggccgc cggtctgcgc
      901 caatccggct atgccgtgac caccacgaca agcccactgg atggcctgcg gatcctgcgc
      961 gagaacgtgt gcgacctggt gatcagcgat gcctacatgc ccgagatgga tggcctcacg
     1021 ctcgcagaga ccgcgaacgg catcttcccc gagctgccca tcgtcatgct taccggcttc
     1081 ggcaccgtag acctgatgcg agaaggacta cgccggggcg cgagcgactt catcacgaag
     1141 ccgtttcgca tgcgtgatat tccgattatc gtcgagcgca acctggaacg caagcgtctg
     1201 gagaaggacc gcctctcgga gcggagcggc cggatcctct tccaggcgat cacctcgttg
     1261 ctcaccgcgg tggatgccaa ggagcactgc acggcccagc actcccgccg cgttgccgat
     1321 atctcgctcc agatcggtgc cgccctgcag cagccggagg cagagatgcg cgtcctcgag
     1381 ctggctgcgt ggatgcacga tgtggggaaa atcgggactc ctgatgcgat tctccgcaag
     1441 ccgggcgggc tcaccgacga cgagtggcag gtgatgcgag agcatgcagc caagggcggc
     1501 gagatcatcg cgcagatcga ggacctccgc tatctcgcct ccgtcatccg ccatcaccat
     1561 gagcgctatg acggcaccgg gtatcccgat cgactgcggg gcgatgcgat ccccctcctt
     1621 tcccgaatta tcgccgtggc cgacgccttc gagacgatca cctcggaccg gggctaccgc
     1681 agcagccgcc cggtttcggt cgcggttgat gagttgaaga ggcatagcgg cacgcagttc
     1741 gatcccgtcg ttgtggagac gctcctgcgc ctgctcgaga gtggccgggt gctctagtcg
     1801 cgcgctcctc ccccagcgat tgtccccctc gttcagggaa gacgggcccg cctccgctcc
     1861 gcccctgccc caccagcccg cggcaccccg gttgcactat tactccatgg gagggctgac
     1921 actcatggag agcgaccacc tttggagact ctacaaggag actggtgacc ttgccgcacg
     1981 ggaggagctc atcctccgcc acgcctttct cgttcgctgc gtcgccgatc ggttggggtt
     2041 caccgggtca gcggggcttg atcgcgatga cgtgatctcc tacggcatca tcggcttgat
     2101 cgatgccgta gagaagtatg gcgcctgtcc cagcccctcg ttcgagtcgt atgctaggct
     2161 ccacatccgc gaggcgattc tcaacgcgat ccgggcgcgg cgcgggcagc ctcggtcatt
     2221 gcacgcctcg gcagacgcca tcgagcaagc ggtgagtgac ctgcagcagc gcctgggtcg
     2281 gcaccccacg atggaggagg tgtcggcagc caccggactc agcgaggaag agatcgaacg
     2341 ggttctcatc tgcctcgaga gcgacggtac cgcgttgtct gagagcggcg acctccccga
     2401 gggcgcttcg gataacctga cactgatgcc gccggacaac ggcgatgacg cgccatcgcc
     2461 ggctgatccg ggaaggacgg cgagggccga gcagcgccgc atcctcatcg aggcgatccg
     2521 caggttgcct cccgaggaac gccttctcct cagcctctac tacatcgagg agctgacgct
     2581 tccggagatc ggacaggtga tgaacctgcc cgaggcacaa gtctcgcaaa tccacacaaa
     2641 ggcgatgatg cgcttgcgcg cgcacctgcg cacgcaggcc gatctcttcg cggcagcctg
     2701 aggagaaacc ggtgatccat ctgacgcgcc tcgatagcgc cccgattgta gtgaactgcg
     2761 acctcgtcga gagcatcgaa gccaccccgg atactcttct ttccttgacc acggggaaga
     2821 agatcctggt caaggagagc gtcgccgagg tggtcgagaa ggtggttgcc ttcaagcgcc
     2881 tcatcgcggc agggccgcga acggatgggg ctatgaacgc ggcacgggat gagcccgcac
     2941 gtggcacgcc atgaatcgcg cgctcccgcg cgcggcgttt ccggggtgct ctgggcactc
     3001 acatgaggtg cgcacgcgcg aacgcgcctg gcttgatgat acacgcccca tcgatcggac
     3061 ccaggcgcag cagatgggca tcactgctga tgaggtaatc ggcgtctccc tccagggcgc
     3121 actccaggaa cttatcgtct tccgggtcat cctgcaccag gtgaagagtg cgcgtggggc
     3181 acacttcctc cgcatcctcc agcagcaggc tcacccgatg tcgaaacgcc tcgctcgtat
     3241 gggccttgcg caggatgtgg tccatctcgc ggcggatggc ggcgctatag agaagctgca
     3301 attggttctc ctcacacgca ctcagcacgc gtgccggcgc actgcgcgga ttccagtagg
     3361 ccgccacgaa gaggttggtg tcgagcacca cccgcaatct cgccggccga accaccatcc
     3421 gcaatgcgac aacccgccct ccttgctcgc ccatactctc cttttcggcg tcactgcgcg
     3481 ccgccctgcc ttgcccggcg cccactggaa ggatccgggc cgccgggagc gaaagggacg
     3541 ctatccggcg cttccagccg cacgcaaatc agaccctgta cctccacaac tcaagccaaa
     3601 gggaaccgca gagagcgtac cttcttggca agctgatgaa aaggacagat ccgaatgccg
     3661 tttcccagcc ccagagagca catgcggcac tggcacttcc cggtcgtcat cctctgcctc
     3721 ctgcttcttc cggcggctga tgctgcagcc gccacgagcc tgacgccggc gaccctgagt
     3781 gccctggtgc atgcgaagaa cgaacctggc gctgccagca gtgcggcact ccacctgcgt
     3841 ggcaacaaca ccctggttct ccttccggtc gcgggcgccc aggcgggcgt ggcgttgcgc
     3901 gcccatgccg gagcgagcgc gtctcagagc gttcgctatc gcgtggttcg cgcgaatggc
     3961 cacgagatcc acgcaggcgc atgcgctcct ggcaaggagg tggcgtggac tatccccgcc
     4021 ggggtgcgct gtctcctgca gattgaggcg ggccaaacgc ccgcgactct ggaggtgcgc
     4081 aacgcaggaa tcggaacgca cgcagcgcgg aacgcaccgg cgcatctcga ccgcggtgcg
     4141 ggccagcagt gctttctcgt gccgaaaggc actcgctcct tcaccctcac cgcccgtgct
     4201 gagggcggag ccgggaaggc cgtcgtgcgt gacccatccg gtcagccggc cgccagcgcc
     4261 acgctctcct cgggcgagga tgcgctcctg aaggtgacgg ttgacgcgaa gaggagcgac
     4321 cgcttctggt cggtatcggc cgagccagcg ggagaaaagg ggttcaccag cgtgcgcctc
     4381 gccttcagcg gcgagatccc gccgtttctg gcgccggatc ccagtggcct ggcgcttccc
     4441 ttcatccact accctgcggt cggcgcactc ctgatgagcg atggcaaccg gtctgcgacc
     4501 atcgaggcgg tgatcgccat agaaccccag gccgatcgct tcgtgcgcgc gcagatgagg
     4561 cgcgcggggg cctcacgcgc gatgtggacg acacggcctt caccgttcac gacgggtcga
     4621 gtgcgcatcc tgctgcctga tcgcctcgcc tctggcgcct atgagctgaa gaccactctg
     4681 caggaccagc aaggggcgat cgtggcaaca cacgcgcagc cgatccacgc cgcgagcggg
     4741 ttcgcgcacg tcgggaagga gcgccccctg ctggagttga tctgccagcc tcaatccgat
     4801 gattccggcg caagctacca gctgcgagcc cgggtgggaa tcgaccccac ctgctactcg
     4861 caagtgcgta tacagagggc cctctatcgg gagggagaga gccaaccagc cttcaaggag
     4921 gagctcggcc accccacggg agaggtattg ccgctgaaga ccccggcggg cgtctcgggc
     4981 ggactctacc ggatggcgct gaacctcgtg agccaggagg gcaagccact cgccacaacc
     5041 tctaggagct tctatctgga gggcaatcgc tgcttcatgg aggtgcctcc acccgcgcag
     5101 gcaaagaaag cgccgctgaa taagagccag cgcacgcgcg gatacgttct cttctcgcgg
     5161 gtttaccacg aggcgttccc ttacaactac gagccgccct gggaagatat cggccggccg
     5221 ctgtacgtct gggcaacgcc cggagaggat gagccggcga cgttcggtgt ttacactctc
     5281 aagcccctcc agaaggcgcg cgtgaaggta ggaaccctct ctcgcgttgg cggaggcggc
     5341 gtcatcaatc cctccagcat cgaggtgcgc ctggctcgct tctggcccca gcgcaccagc
     5401 gatcacagtg ccacctaccg cctgatcccg gagctgctgg agccggtcac cacggcgacg
     5461 atcccaaccg gtcgcatctc gcaatactgg ctcacgcttc acattccgcc cggtaccccc
     5521 gcgggcgatt accggggaat gatcacctat caaccggaga atgccccggc gtcgcagctc
     5581 aagctgctgg tgaaggtgct cccattccgg ctggagcggc cagcctcgcg gacgtggggt
     5641 ctctacgccg acagcacgcg gtggcgcggc gctacagacg ccaccatcga gcgcgagatg
     5701 cgcgacatga aggcgcacgg gatcgactcg ctcgcgctct ccgcgcttgc ccacggcacg
     5761 ctgacccacg cgggcgagaa gctcacggtt gatttcacgc cactctcgcg cctgatgggg
     5821 atctacaagc gagtggggtt gcgcgggccg gtgatcctcg atctgcgcga tctcccccag
     5881 gtgatagcgc aagctggggg tgccagcacg aagccggatg accctgtagt gcaacgcctg
     5941 gccggtatcg tgctctcgga gctggcgatg cgcgccgaaa cggagaagtg gccgcaggcg
     6001 gtctaccaac tcgcggacga gctgaacgcc cattccgcaa gctcgctggc cacggcacgg
     6061 gcactcctct cgcttggcaa gaccgcgtca ctccccactc tggccttcct cgctagtccc
     6121 caggacggga tgagagagct cgacgcgctc atcgatctcc cgtgctacgg cgccgaggcc
     6181 gtcgccggcg acaaggacgg cgccctgcgc aaggcgctca aggaggcgaa caagcccctc
     6241 tggtggcacg ggagcggctg cacctccccc cggctagagg gaaacctgac cgccaaccgc
     6301 tacctgactg gcatcgcatt ctggcgctcc ggcgccgccg cgcactggat ccagacctac
     6361 cagcgcccca tgggcaacgc ctactccgat ttcgatggcg atggccagat gacggggaag
     6421 gacctctgcc tgacctaccc ggcgccagcc ggcggggcgg cagtgcccac catccagtgg
     6481 gaagggatcc gggagggcta cgacgattac cgctacctct acacgttcca gcagatggtc
     6541 gcgcgcgcga agcgctccgg ccacgaacgc ggagcgaagt acgccgccca ggtggaaaaa
     6601 gaggcgcagg agcggctgga acgtctcccc tggggcgccg agccagaagg cttgaccgcc
     6661 tacgaggccc acgccttccg caagtggctc tcgaaataca tgggcacgct cgtcaataag
     6721 ctgaactcgt gagctgagat tccgagccac ggaggcaggg cgcaacccgg cgagcggcga
     6781 taaatcaccg cgctgcttgc gagcgaagcc ctgccgggct gggttgggga tactcccaga
     6841 tgtctcaaaa cggcgctggc tggggggaga agcctcaaag gggctttgcc ggaaagcagc
     6901 gcggagcttc gagctccgcg cgtgcgggcg ccctctccct atgtcggaat cccaagactc
     6961 gtgaggcacg aggccaggcg gtcacgcgcg aagacggccg atgcgctcca ggaactcggc
     7021 gagggcctcc tcccaggggc gaagtacctc ggcgcgtccc gtcagctcca ggacgtaggg
     7081 ccgaagcacc gagtaggcgg gccggcgcgt ggggctgggc cactcgtcgg atgagatcgg
     7141 acgcacgatg cccggatcca gcccggccat ctcgaggagg cgcgcggcga agccatgcca
     7201 ggagacctct ccccggttgg cggcgtggta ggttccatag agcggttgct ccaagatccg
     7261 cgcgatggcg cgtgacaggt cgcgcgtata cgtcggcacg cccacctgat ccgagacgac
     7321 gcgcagctct tcgccggcat cggcgcgctg caagatggcg agcgggaagt tcttgccatg
     7381 ctccccatag agccattgcg tgcgcacgat gtaatgacgt gggcaaagcg agcgcaccag
     7441 ctcctccccc gccagtttgg aggcgccata gatgctcaac gggtttggcg cgtcaaactc
     7501 cgtatagggg cgccctttct cgccatcaaa gacgaagtcg gtgctgatcg cgacgagggc
     7561 ggcgcccacg gcatgacagg cgcccgccac cgcggccgtt ccaagagcat tcacgcggta
     7621 ggccccgtcc gggtcgcgct cgcacccatc cacgttcgtg tacgcggcgc agtggacgac
     7681 ggcatcgggc cgaacggctt ccaggagagc gtggaccgcg cgcgcgtcgg tgatatcgac
     7741 gcagtgatcc ggcccgcgcc ggcccgcgcc aatcacatca tgctgctcgg aaaggatctc
     7801 caccaagtcg cggcccagca ttccagccgc gccggtgacg agaacgcgca tctcggggta
     7861 cctcccaacc accgggtgtc gcccccacta tagcaggggg ctacccacct cgtcaagcaa
     7921 gcgagcgagg gcttgccgat gctgccccga tgcggtagaa tgagcgcagg gcacggtaac
     7981 gcgccctgga tcacctggaa ggaacctgac atgcagttgg tatcggttgt tttggtcggt
     8041 ctggccgcgg gcgcgctgag cgtcctgctg ggggttggcg gcggtgtgat cat
//
LOCUS       DAOSCV010000059         8089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5783_length_8089_cov_3.442619, whole genome shotgun sequence.
ACCESSION   DAOSCV010000059 DAOSCV010000000
VERSION     DAOSCV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5783_length_8089_cov_3.442619"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="PLY56_02555"
     CDS             complement(<1..1270)
                     /locus_tag="PLY56_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20391.1"
                     /translation="MRGPQRVAAAVAAALASACAVWEAGRVLGLEPFTRASLSMIALG
                     YASATMAHWWPVAAIRALVLAALVGVAFAALRPNPPDPILDCAQVLQQKSGASILVGL
                     VFAVVFLSFVAGTYLRGRRVPMVATAVPALSIYGLAGVFTSGFSLQVSFSGFLFAAVY
                     AMAYEHLLSQSTPATGSFPPRRSARSALGLAAGYLALLLPLSFPLASLSQRMTPGLTP
                     AERDRLRRTLFSTDDDPGYSRLSETVSLAGGMRTTGEQALMRVKTTTPGLLRGRIYDV
                     YERGCWSQSQLIETSIPLGDGPVGRRGRRAARRHAGGLDASDAREAAREERRRYFTHP
                     PRHSRPPQEHARRVTQQMDLLGRVPGSVLCAGIPLEVITTREDLTVDVTGLVFGKTDW
                     REGDTYTVVSEVPDVPEPLLRRAPGGQPPEE"
     gene            complement(1270..2235)
                     /locus_tag="PLY56_02560"
     CDS             complement(1270..2235)
                     /locus_tag="PLY56_02560"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOJ20392.1"
                     /translation="MRQRRAAKGRRLPGARGHFACSIWYCACVCLIAMLLLAGMAALH
                     AAPNAPAAATKGNRVPLTPGRGPRVSLDVHEIDVRTVLTVLGENAQVRFSLAPDVRGK
                     VTCSYRDLALEEMLDVILPPLGLRWKRLAAQQYRVERAVEQKTGKALLSSPRGWDPAG
                     LKALYYNDPACELADGSLSALLAVPPEGVTVVTRTDARPGGIWSETGPAPGVGPAQWS
                     ARWTGRLYVPADDSYTFSFDPIHGAALLLLDGKVVVDSWRIAPRGKTAGSPVKLSRGW
                     HDVVIEYHQAGPTGALDLKWQASWLPLETIGIHASGKPAQGRTTP"
     gene            complement(2298..3371)
                     /locus_tag="PLY56_02565"
     CDS             complement(2298..3371)
                     /locus_tag="PLY56_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOJ20393.1"
                     /translation="MSLLPMPGALADGRAPGDVTGTEKPAGLSRAKLVAPQGSPIVAS
                     RPRMRTARVLPPRRDVVAGRTAPRPGLGSRVPEGAPNRPAGSPDPGLPAAPGGTEPVP
                     PTIASPKAPHDGDPEAPPFAEGTMAAATGTPEAPDAVRQELPAPDFDLASRASQPDLP
                     GTRQALGQQGEGIPRGRRGTGGATHNRGFLGEGLLAAYYADPPRAKGDYPLNTPFTFP
                     TFTRLVTTRIDPAINFVWDMDPPAPDVPPTYFSVRWTGKLLVPATDTYVFYLDALDDG
                     ARLYIDGQMVIDSWMIQQGISVIRAVPLEAGIHDIRVDYCQGPEHHASIRLAWSSSSF
                     GKEIIRARPSPAGPHLVAGLPKK"
     gene            4167..4520
                     /locus_tag="PLY56_02570"
     CDS             4167..4520
                     /locus_tag="PLY56_02570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ20394.1"
                     /translation="MRVVHHEEIGAKPVAAEGAERATIREIFTAETGAPTFAMRVFDL
                     APGGCTPLHSHPWEHEVYVLDGSGEVEGAEGATPLSPGMAVYVAPNEVHRFRAHAGSP
                     LKFLCLIPVQQVCCR"
     gene            4546..5370
                     /locus_tag="PLY56_02575"
     CDS             4546..5370
                     /locus_tag="PLY56_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ20395.1"
                     /translation="MSLCLTSALALAGLAGTAYAVGLERRWIQVTRCDVPLPDLPAEL
                     DGFTIAHLSDIHYGPMMLPGLIERAVARVNALQPDLVALTGDFVDGKASEVEPAARLL
                     GGLRARHGVWAVLGGHDITASERVACRAYPENGIRLLRNAAAPLIESDPPFWIAGVRD
                     NSEYYLDRLKAALSGVPERARILLLAHSPDIIPEAREAGIDLVLSGHTHGGQICAPLY
                     GAILTETRLGRPVARGLNRFGNTWIYTCRGLGVVRLPARFLCRPEIALLRLVRASR"
     gene            5461..6507
                     /gene="argC"
                     /locus_tag="PLY56_02580"
     CDS             5461..6507
                     /gene="argC"
                     /locus_tag="PLY56_02580"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ20396.1"
                     /translation="MLRVGVIGARGYTGAELVRLLCKHPDVELTYLTSESQQGISISA
                     IYPSLRGHIDLACEAFDVETACAKADLFFYALPAGEAMKSAGALLAAGKRAIDLGADF
                     RLKDPERYRQWYKGEPAPAELVSAAVYGLPELYRGQLREARLVANPGCYPTSAILGFA
                     PLLAKGWIDPASIIIDAKSGVSGAGGRSTMKPEYSFPHINDNFRAYGLVTHQHTPEIE
                     QEFARLSGVNAPVQFTPHLVPMTRGILMTGYASLAREADTPGLLAEYEAFYAGAEFVQ
                     VLPEGTLPETKYVVASNQCHIGVRVDPRTRRVIVVCAIDNLMRGAAGQAVQNMNVMCG
                     FPERHGLDLPALYP"
     gene            6585..7799
                     /gene="argJ"
                     /locus_tag="PLY56_02585"
     CDS             6585..7799
                     /gene="argJ"
                     /locus_tag="PLY56_02585"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOJ20397.1"
                     /translation="MEEIAGGVTAPRGYRACGVCCGLKVSGKPDLALLVSDVPANVAG
                     VFTTNEVKAAPVLLSRQVVARGAARAVIINAGNANCCAPRDRENAERMQAAAAAALGL
                     SPQEVLVCSTGVIGHELPVEKIEAALPAAVSELRPEGGPGAALAIMTTDTRPKEIAVE
                     VQLPEGSVRVGGMCKGSGMIAPNMATMLGFLTTDAAVEASTLQAMLAEIAGRTFNCVT
                     VDSDTSTNDTLLLFANGAAPAAGAFAAGHPKREENLRLLAEAVEHVCTYLAREIARDG
                     EGATRLVTVTVRGAANEAAADAVARTIAESPLVKTAIFGRDPNWGRILMAAGRAGVPL
                     DPARVTVALGDVTVFRHGLAVAFDPDAARAALTADEVQIQVDLGAGEATRRVWTCDFT
                     YDYVKINADYHT"
     gene            7886..>8089
                     /locus_tag="PLY56_02590"
     CDS             7886..>8089
                     /locus_tag="PLY56_02590"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727984.1"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ20398.1"
                     /translation="MSSVEERAQTLIEALPYIRRYHGKTVLIKYGGNAMIDEKLKEAV
                     ITDVVLMHYVGMRPILVHGGGPEI"
BASE COUNT         1316 a         2613 c         2872 g         1288 t
ORIGIN      
        1 cctcctcggg gggctggccg cccggcgcgc gtcggagcag cggctcgggt acatccggca
       61 cctcggagac caccgtgtag gtatcgccct cccgccagtc tgtcttgcca aagactaggc
      121 ccgtcacatc aacagtcaga tcctcgcggg tggttatcac ctccaggggg atgccagcgc
      181 agaggacgct tccgggcacc ctgccaagga gatccatctg ctgggtaacg cggcgggcgt
      241 gctcttgtgg cgggcgcgaa tgccggggcg ggtgggtaaa gtagcgccgt ctctcctcgc
      301 gagccgcttc cctcgcgtcc gaggcgtcca gaccgcccgc gtggcgccgc gcggcgcgtc
      361 gccctcgcct tccgaccggg ccatcgccga ggggaatgct ggtctcgatc agctgggact
      421 gcgaccagca gccccgctcg tagacatcat agatgcgccc gcgcagcagg ccaggcgtcg
      481 tggtcttcac ccgcatcaac gcctgttccc cggtcgtgcg catcccgccg gccaggctga
      541 cggtttcaga aaggcgcgag tagccggggt catcgtccgt agagaagagg gtgcgccgca
      601 agcggtcacg ctcggcaggc gtcaggccgg gagtcatgcg ctgggagagt gaagcgagcg
      661 gaaacgaaag gggcaacagc agagccaggt agccggcggc aagcccgagg gcggaccttg
      721 cgcttcgccg cggggggaac gaccctgtgg ctggggtgct ctgcgagagg agatgctcat
      781 acgccatcgc gtaaacggcg gcgaagagga agccagagaa ggagacttgc aggctgaagc
      841 ccgaggtgaa gacccccgcg aggccataga tggagagggc ggggaccgcg gtggccacca
      901 tcggcacgcg ccggcctcgg agataggtgc ctgccacgaa tgagaggaaa acgactgcga
      961 agacgagccc cacgaggatg ctggcgccac tcttctgctg caacacctgg gcgcaatcaa
     1021 ggatgggatc gggcggatta gggcggagcg cggcgaaggc aacgccgacg agcgccgcca
     1081 gcacgagggc ccgaatcgcc gccaccggcc accagtgggc catcgtcgcg gacgcgtagc
     1141 ccagggcgat catcgagagg gatgcgcgcg tgaacggctc cagccccagc acgcgcccgg
     1201 cctcccaaac cgcgcaagcc gaggcgagcg ctgcggccac ggctgccgcc actctctgtg
     1261 ggccgcgcat caaggggtcg ttctcccttg tgccggcttg ccggaagcgt ggatcccgat
     1321 cgtctccagg gggagccacg aggcttgcca cttcaagtca agggcgcccg tcgggcccgc
     1381 ctggtgatac tcgatgacca cgtcgtgcca gccgcgcgag agcttcacgg gcgagccggc
     1441 cgttttcccg cgcggggcga tgcgccagga gtcgaccacc accttcccat ccagaaggag
     1501 gagcgccgcc ccgtggatgg gatcaaaaga gaaggtgtag gaatcgtcag ctggcacgta
     1561 gagacgacct gtccagcgcg cgctccactg agccgggccc acgccgggag ccgggccagt
     1621 ttcgctccag atcccgcccg ggcgcgcgtc ggtgcgcgtc accaccgtga ctccctcggg
     1681 cgggacagcc aggagcgccg aaagcgagcc atcggcgagc tcgcaggcgg gatcgttgta
     1741 gtagagagcc ttcaacccgg ccggatccca accccggggc gagctcagga gtgccttgcc
     1801 cgtcttctgc tcgacggcgc gctcgacgcg atactgctgc gcggccagcc gcttccatcg
     1861 aagacccaac ggggggagga taacgtcgag catctcctcc agggccaggt ccctgtagct
     1921 gcacgtgacc ttgccgcgga cgtcgggcgc gaggctgaag cgcacctgag cgttctcccc
     1981 cagcaccgtc agcaccgtgc gaacatcaat ctcgtgcaca tcaagcgaga cgcgtggccc
     2041 gcgtcctggc gtgagcggga cgcggttgcc cttggtcgcg gcagcggggg cattcggggc
     2101 cgcatggagc gccgccattc cggccaacag gagcatggca atcaggcaga cacacgcgca
     2161 ataccagatg gagcaggcaa aatgcccgcg cgcgcctggc aggcgccgcc ctttggctgc
     2221 gcgccgttgt ctcacctcct ggcaggttgg gtccactagg tgtcgctgtc ggttgccgcg
     2281 catgaagccc tcggagttca ctttttcggc aaccccgcga ccaggtgcgg gcctgcggga
     2341 gatggtcgcg cccggatgat ctccttcccg aagctggagg aactccaggc aaggcggatg
     2401 gaggcatggt gctcgggccc ctggcagtag tctacccgga tatcgtggat gccggcttcc
     2461 aggggaacgg cgcgaatgac ggagatgccc tgctggatca tccaggaatc gatcaccatc
     2521 tgcccatcga tgtagaggcg agcgccgtca tccagggcat ccaggtaaaa gacgtacgta
     2581 tcggtggccg gcaccaggag cttcccggtc cagcgaacgc tgaagtaggt ggggggcacg
     2641 tccggcgcgg gcgggtccat gtcccatacg aagttgatcg ccggatcgat gcgtgttgtc
     2701 accaggcgcg tgaacgtcgg gaaggtgaac ggcgtgttga gcgggtagtc acccttggcg
     2761 cgcggcggat cagcgtagta ggcggccagg agcccctcgc cgaggaagcc ccggttgtgc
     2821 gtcgcgccgc ccgtgccgcg ccggccgcgc gggatcccct cgccttgctg gccgagcgcc
     2881 tgcctggtac ccggcagatc cggctgcgaa gctcgggaag ccaggtcgaa atcaggggcg
     2941 ggaagctcct ggcgaaccgc gtcgggggct tccggcgtgc cggtggccgc ggccatcgtt
     3001 ccctcggcga atggcggcgc ctccgggtcg ccgtcatggg gcgccttcgg agaggcaatg
     3061 gtcggcggca ccggctcggt gccgcccggt gccgctggaa ggcccggatc aggcgagcca
     3121 gctggccggt ttggcgctcc ctcgggtacg cgagatccca gccccgggcg cggggcggtg
     3181 cgaccagcga ccacatctcg ccgcggaggg aggacgcgcg cggtgcgcat ccgcgggcga
     3241 gaggcgacga tagggctgcc ttggggcgcg acgagcttcg cgcgcgagag tcctgccggc
     3301 ttctcggtgc ccgtgacatc tccaggggcg cgcccgtccg ccagcgcgcc cggcatgggg
     3361 agaagcgaca cctcgacgat cccgacggag cgtggccgtg agctccgaag ggcgctcagg
     3421 agcaggatgc ccccgagcgc gtgtagcatg gccgatacta agagacagcg cctgattatc
     3481 cttcgctcca tactacacca cccgtgttgc gcacgctcag atgcgtgctt gcatagcaat
     3541 atagactctc cccaggggca gaagttccag gccgggattc cgagagaagg gatccggtct
     3601 gggcacagag cctcccggct ccaatgccca gctctctcca gcctcttctc ccatcgcccc
     3661 gcggaccgca caacttagcc caggatgtcc gcaccctggg ttatgcaccg gcatagatag
     3721 ccacgaccgc gaagcggtcg cacaagccgc tactccgtat tatcgcaccc cttcggggtg
     3781 ccaggtattc ttctggacgc gagacccagg atcgggagac cctgggctat cctgtgtgac
     3841 cgcttcgcgg tcctccgcga gcgccaccca gatggccatc ctcgggctct ggataagatc
     3901 gcacaatccc gggtgcgcgc acgttcccat ccgtgtccgt caccttcatc cgtggcatcg
     3961 atgccgagcc ccctgagatt ccgacataag gagagggcca cctgcacgca cggtggttta
     4021 tcgctgcgcg ccgggttgcg tgctgcctcc gaggcttcgg attccttatg tcggaatccc
     4081 agccgggcca ccgcatcgtt tgccctggct gccccctgtg tggtagaata acgcctagag
     4141 agtgagagga gaggcaggaa gaggcgatgc gggtcgttca tcacgaggag attggggcga
     4201 agccggtggc cgccgagggc gcggaacggg caacgatccg agagatcttc acggcggaga
     4261 cgggtgcgcc tacctttgcc atgcgtgttt tcgacctggc gccaggaggc tgcacgccgt
     4321 tgcattcgca tccctgggag cacgaagtct atgtgctgga tggatcgggc gaagtggagg
     4381 gcgccgaggg tgcgacgccg ctctcgcccg gaatggcggt ttatgtcgcg ccgaatgaag
     4441 tgcatcgctt ccgcgctcac gctggctcgc cgttgaagtt cctctgcctc atcccggtgc
     4501 aacaggtgtg ctgtcggtga gcgctccggg tgagggtctt ggcgaatgag cctctgtctc
     4561 acgagtgccc tggcgctggc cgggcttgcg ggcacggcct acgcggtggg cctggagcgc
     4621 cggtggattc aggtgacgcg ctgcgatgtg cccctgcctg acctccccgc ggagctcgac
     4681 ggcttcacca tcgcgcatct gagcgacatt cactatgggc cgatgatgct gcccggcctg
     4741 atcgagcgcg cggtggcccg ggtgaacgcg ctccagccgg acctagtggc gctgacgggc
     4801 gatttcgtgg atggcaaggc gagtgaggtg gagccggcgg cgcgcctgtt gggcgggctg
     4861 cgcgcgcggc atggcgtgtg ggcggttctc ggcgggcatg acatcacggc gagcgagcgc
     4921 gttgcctgcc gggcctaccc cgagaacggg atccggcttc tgcgcaacgc ggcggcgcca
     4981 ctgatcgaga gcgatccgcc cttctggatc gccggcgtgc gcgacaattc ggaatactac
     5041 ctcgaccggc tgaaagcggc gctttctggg gtgccggagc gcgcgcgaat ccttctcctg
     5101 gcccattcgc ccgatatcat cccggaagcg cgcgaggcgg ggatcgacct ggtcctgagc
     5161 gggcataccc acggagggca gatctgcgcg ccgctctacg gggcgatcct gacggagacg
     5221 cgcctgggcc ggcctgtcgc gcgcggtctc aaccgtttcg ggaatacctg gatctacacc
     5281 tgccgcgggc tgggcgtggt ccgcctgccg gcgcgttttc tgtgccgccc ggagattgcc
     5341 ctcctccgcc tggtgcgggc cagcaggtaa cacgaggcgc cgccgcgaag ggatgggtgt
     5401 tgcctcaccg aggcgcgagc gccgacgggt agatggcttt ggaataatgg agttgtggct
     5461 atgcttcgag ttggagtgat cggcgcgcgc gggtataccg gcgcggagtt ggtgcgcctt
     5521 ttgtgcaagc accccgatgt ggagttgacc tacctcacct ccgagagtca acaggggatc
     5581 tcgatcagcg cgatctaccc cagtctgcgc gggcatatcg acctggcgtg cgaggcgttc
     5641 gacgtggaaa ccgcgtgcgc gaaggccgac ctcttctttt acgcgctgcc cgcgggcgag
     5701 gcgatgaaga gtgccggcgc gcttctcgcc gctggcaagc gggcgattga cttgggcgcc
     5761 gatttccgcc tgaaggatcc ggagcgctat cgccagtggt acaagggcga acccgcgccg
     5821 gcggagctcg tctcagcggc ggtttatggc ctgcccgagc tctaccgcgg tcagctgcgc
     5881 gaggcgcgcc tggtcgccaa tccggggtgc taccccacct cggcgatcct tgggttcgcg
     5941 cccctgcttg ccaaaggctg gatcgatccg gcgagcatca tcatcgatgc caagtcgggc
     6001 gtctccggcg ccggggggcg gtcgacgatg aagccggaat actccttccc gcacatcaac
     6061 gacaacttcc gggcctacgg gctggtgacg caccagcaca cgccggagat cgagcaggag
     6121 tttgcccggc tctctggcgt gaacgccccg gtgcagttca cgccccacct ggtgccgatg
     6181 acgcgcggga tcctgatgac cggctatgcc tcgcttgcgc gcgaggccga tacacccggc
     6241 ctcctggcgg agtacgaggc gttctacgcg ggcgcggagt ttgttcaggt cctccccgag
     6301 ggcacgctgc ccgagacgaa gtacgtcgtg gcgtccaacc agtgccatat cggcgtgcgt
     6361 gtggacccgc gcacccgccg cgtgattgtc gtttgcgcca ttgacaacct gatgcggggc
     6421 gccgcgggcc aggccgtcca gaacatgaac gtgatgtgcg gtttcccgga gcggcatggc
     6481 cttgacctgc cggcgcttta cccctaatcc gcgcatccct cgcgcgtgat ggagccagag
     6541 cgagggcacg cggtttttct ggcaagagtt tgggagcgcg attgatggaa gagatcgctg
     6601 gcggcgtgac ggccccgcgg ggctatcgcg cctgtggagt ttgctgcggt ctgaaagtga
     6661 gtggaaagcc cgacctggcg ctgctcgttt cggacgtgcc ggcgaacgtc gccggagtgt
     6721 tcacgaccaa tgaagtgaag gcggcgccgg tcctgctgag ccggcaggta gtggcgcgcg
     6781 gcgcggcgcg ggccgtgatc atcaatgccg ggaatgccaa ctgctgcgcg ccccgggatc
     6841 gcgagaacgc ggagcggatg caggcggctg ctgcggcggc gctcgggctg tcaccgcaag
     6901 aggtgctcgt ctgctccacg ggcgtgatcg ggcacgagct tccggtggag aagatcgagg
     6961 cggcgctgcc cgcggcggtt tcggaactgc gcccggaggg tggcccaggc gcggcgttgg
     7021 cgatcatgac cacggatacc cggcccaagg agatcgcggt cgaggtgcag ctgcccgaag
     7081 ggagcgtgcg cgtcggcggg atgtgcaagg ggagcggcat gattgccccg aacatggcga
     7141 cgatgctcgg cttcctcacc accgacgcgg cggtggaggc gtctaccttg caggcgatgc
     7201 tcgccgagat cgccgggcgc accttcaact gcgtgacggt ggatagcgac acgagcacca
     7261 acgacacgct cctcctcttt gccaacggcg cggcgccggc ggccggcgcg ttcgcggccg
     7321 ggcatccgaa gcgcgaggag aacctccggc tgctcgcaga agcggtcgag cacgtctgca
     7381 cctatctagc gcgcgagatt gcccgcgatg gcgaaggggc tacgcgcctg gtgaccgtca
     7441 ccgtccgggg cgcggcgaat gaggccgcag cggatgccgt cgcgcgcacc atcgccgagt
     7501 ccccactcgt gaaaacggcg atctttggcc gggatcccaa ctgggggcgc atcctgatgg
     7561 ctgccgggcg cgctggcgtc ccgctcgacc cggcgcgcgt cacggttgcc ttgggcgatg
     7621 tgaccgtctt ccggcatggc ctggcggttg cgttcgaccc ggacgcggca cgcgcggcgc
     7681 tcaccgctga tgaggtgcag atccaagtgg acctgggcgc gggcgaggcc acgcggcgcg
     7741 tctggacctg cgatttcacc tacgactacg tcaagatcaa cgccgattac catacctagg
     7801 agaccgcggc gccgggcagt tgggccagcc ggtggcggac cagccgcgcc ggcctggagg
     7861 tccggggccg gtggagcgag agagcatgag cagcgtagaa gagagagccc aaactttgat
     7921 cgaggccctg ccctatatcc ggcggtatca tggcaaaacc gtgctgatca agtacggcgg
     7981 caacgccatg atcgatgaga agctgaagga ggcggtgatc accgacgtgg tgctgatgca
     8041 ctacgtgggg atgcgcccga tcctggtgca cggcggcggc cccgagatc
//
LOCUS       DAOSCV010000060         8027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5835_length_8027_cov_3.166834, whole genome shotgun sequence.
ACCESSION   DAOSCV010000060 DAOSCV010000000
VERSION     DAOSCV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5835_length_8027_cov_3.166834"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLY56_02595"
     CDS             complement(<1..567)
                     /locus_tag="PLY56_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HOJ20399.1"
                     /translation="MSNSPVRGGALPSGSRVVQSLAQVGAMRLLRCAPKDGFTNMAVD
                     EAILRAHAAGEAPPTLRLYAWNPPAISIGYFQRAQRDINLEACARAGVDVVRRLTGGR
                     GVLHADEVTYSIVIAERAFAGAGVPEAYRILSEGLIAGLRRLGLEAHLAPGQRAHPGP
                     SGANCFAAAASCDLVVNGLKICGSAQVRR"
     gene            complement(560..2026)
                     /gene="gcvPB"
                     /locus_tag="PLY56_02600"
     CDS             complement(560..2026)
                     /gene="gcvPB"
                     /locus_tag="PLY56_02600"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOJ20400.1"
                     /translation="MSEPLIFELSRPGRVAFSLPECDVPERPVEELIPAGQIRGDLPL
                     PEVSEPQVVRHFTRLSRMNYGVDMGFYPLGSCTMKYNPRVNEAAAALPGFATAHPLAG
                     EEHSQGALQLLYELQEMLAEIAGMDAVTLQPVAGAQGELTGLLMIRAYHLERGDTART
                     RILVPDSSHGTNPATAARCGYTITSVPSNDRGRVDLEQLRAAMDSQVAALMLTNPNTL
                     GLFEDEVLEVTRIVHEGGGLVYMDGANMNAILGIARPGDLGFDVMHFNLHKTFSTPHG
                     GGGPGAAAVAAKAHLEPYLPVPRVVRREGAYALSEEHPRSIGRMHTFYGNFANLVRAY
                     TYLRTLGPEGLRLVAENAVLNANYLLALLKEDYEVPYGDRCMHEFVASAQQQAEKGVR
                     ALDIAKRLIDHGFHPPTMYFPLIVREALMIEPTETESIETLDAFAEAMREIAREAREQ
                     PEVVHDAPHPTPLVTVPVSRVDEVAAARHPNLRWTADE"
     gene            complement(2029..3381)
                     /gene="gcvPA"
                     /locus_tag="PLY56_02605"
     CDS             complement(2029..3381)
                     /gene="gcvPA"
                     /locus_tag="PLY56_02605"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOJ20401.1"
                     /translation="MRFLPGTEADRREMLHRIGASSVEELFAEVPPEGRVQGLLRLPP
                     ALSEPELLAHLGDLAARTTGTAEYCCFLGAGVYDHYIPAVVPALVGRTEFLTAYTPYQ
                     AEISQGLLQSIYEYQTLVCQLTGMDAANASMYDGATALAEAAIMAGAATRRNRFLLPR
                     TIHPYFQHVVATYLAPLPYELVQIPQASDGGVDREALERELDERVAAVLFQHPNFYGC
                     LEDARELCDAAHRVGALAIAAVDPISLGLLTPPGEYGADIVVAEGQSLGNSLSFGGPL
                     LGLMACRSRLLRNLPGRLVGATTDRDGRVSFCLTLQAREQHIRREKASSNICTNQALN
                     ALAATVYLCTLGKQGLRRVAELCLQKAHYAHSRLQADAGFTPIFSRPFFKEFALKAPE
                     PVASLNERLRAAGILGGLDLGRLSPELEGATLLCVTERRTREQIDTMVAVAAGDTERS
                     "
     gene            complement(3389..3775)
                     /gene="gcvH"
                     /locus_tag="PLY56_02610"
     CDS             complement(3389..3775)
                     /gene="gcvH"
                     /locus_tag="PLY56_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOJ20402.1"
                     /translation="MNLPPELRYAKSHEWVRVEGDLATIGITDYAQDQLGDVVYIELP
                     EVGRTLQQEETFGSIESVKAVSELLAPVSGEVVEVNEELLDTPETVNEDPYSAGWMII
                     VRMDDPSEVKDLLTAEQYQAFVEEEG"
     gene            complement(3848..4933)
                     /gene="gcvT"
                     /locus_tag="PLY56_02615"
     CDS             complement(3848..4933)
                     /gene="gcvT"
                     /locus_tag="PLY56_02615"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ20403.1"
                     /translation="MKQTILNAEHRRLGATLVEFAGWEMPVSYSGILEEARAVRQAAG
                     LFDVSHMGVLQLCGTNARAELEHLVTCALRDLPVGRVRYGLMLAPNGGVLDDLLIYSL
                     ASDDLWVVANAANASRDLQWIQEHVSPGVQVTPHFGEVAILALQGPRALGIAGAATGQ
                     EFTTLRRFGFARWSFDGAEVITSRTGYTGEDGLEFFCPAGKALALWERLLDAGRDSGL
                     LPCGLGARDVLRIEAGNALYGHEVNEETNPVEAGLMWAVNLDKGDFVGREAVLDARSA
                     PSTRRLTGLVMEEKAIPRQGAPAGTDTEATGVVTSGTFSPALDRGIALAYLPPEEAVP
                     DRAAWVELRGRRRRARVASLPFVQRKR"
     gene            complement(4930..5292)
                     /locus_tag="PLY56_02620"
     CDS             complement(4930..5292)
                     /locus_tag="PLY56_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ20404.1"
                     /translation="MNLTIDISRVDNATILTLAGELDAYTSLELRETVVRLVEEGVRS
                     LIVNLSKVDFIDSVGLGTLVGCLKRSAEHGGTMRLVGLNPQIQKVFDITGLSKIFDIH
                     TTNEEALKHVTPAAAGNS"
     gene            5940..6980
                     /locus_tag="PLY56_02625"
     CDS             5940..6980
                     /locus_tag="PLY56_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20405.1"
                     /translation="MSDKVRIAFVGCGGHASGALYPQFARIAEIDLVAVVDQIEEKRL
                     VAQRTWGARRAYASVEEMLDKEKPEGVCISGPPQMHYEVGLQVLERGIPIFVEKPSAI
                     DFQHAYDLAEAARKAGTFGMTAFMKRFATAYEKAKEITQREEFGPVSMVSVRYTHGAY
                     PSIWGIEDRALVCLIGNQCHAFDLARYFGGDVTELYARLCDRGEGCFAFAVTLQFENG
                     AVGLLNSNATDDPSWNIRETLNVSGLLCSVDVEDMIHVNYRPRERWAPELGPWREGLY
                     ALPHAVSFNLGGRWLGYEGELRHFAQCIRGEVENRSSLYDGAASLAIAEAIWESAKTG
                     KPVRPKSHKPGQ"
     gene            7195..>8027
                     /locus_tag="PLY56_02630"
     CDS             7195..>8027
                     /locus_tag="PLY56_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOJ20406.1"
                     /translation="MLKHPQLTAQRLERCLNNEIRPHIYQDLTPLEVAVFQCPDPIPY
                     AEAIRQEYRSVSVGFRWGPVWSTAWFRLRGAIPEAFAGHEVVALIDTSSEALVWDGDC
                     PGQGLDANRSDYLLAESAVAGQPVELYVEAAGNHLFGISAMGQTSGLARVPEPFELRQ
                     AHLARFDRAHWNLFNDFRVLLDLMKALPEGDPRKAQLLYDLNECANRYLTGQGPKAAR
                     EALAQALSLPARASSTRASAIGHAHIDVAWLWPLRETVRKCARTFSTALRYMERYPNY
                     K"
BASE COUNT         1352 a         2665 c         2687 g         1323 t
ORIGIN      
        1 acggcgcacc tgggcgctgc cgcagatctt caatccattc acgaccagat cgcaggacgc
       61 agcggcggcg aagcagttgg ctcccgaggg ccccggatgc gcgcgctggc cgggggccag
      121 atgcgcctcc agacccaggc ggcgcagtcc ggcaatcagg ccctccgaga ggatgcggta
      181 ggcctcagga acgcccgcac cggcaaaggc ccgctcggcg atcacgatgc tgtaagtcac
      241 ctcgtcggca tgtaagacgc ctcgcccccc ggtcaaccgg cgcaccacat cgacgcccgc
      301 gcgcgcgcac gcttcgaggt tgatgtcgcg ctgggcacgt tggaagtagc caatggagat
      361 cgcaggtgga ttccaggcgt agaggcgcag cgtcgggggg gcctcgccgg cggcatgcgc
      421 acgcagaatg gcctcatcca cggccatatt ggtgaagccg tctttcggag cgcaccgaag
      481 gaggcgcatt gccccgacct gcgcgagcga ctggacgacg cggcttccac tcggaagcgc
      541 tccgccgcga acgggagagt tactcatcag cggtccagcg caggtttgga tggcgagcgg
      601 cagcgacctc atccacgcga ctcaccggga cggtgacgag gggcgttgga tgcggggcat
      661 cgtgcacgac ttccggctgc tcgcgggcct cgcgcgcgat ctcgcgcatc gcctccgcga
      721 aggcgtcgag cgtttcgatg ctctccgtct ctgtcggctc gatcatcagc gcctcgcgca
      781 cgatgagggg gaagtacatc gtgggcgggt ggaacccgtg gtcaatgagg cgcttggcga
      841 tatccagggc gcgcacgccc ttctcggcct gttgctgcgc cgaggcgaca aactcgtgca
      901 tgcaccggtc gccatacggg acctcgtaat cctctttgag gagggccaag aggtagttgg
      961 cgttgaggac cgcgttttcg gcgacgaggc gcaggccctc ggggcccagg gtgcggaggt
     1021 aggtgtaggc gcgcaccagg ttggcgaagt tgccgtagaa ggtgtgcatt cgcccgatgc
     1081 tccgcgggtg ctcctcggag agtgcatacg cgccctcccg gcgcaccaca cggggcacag
     1141 gaaggtaggg ctccaggtgt gccttcgcgg ctacggccgc agcgcccggc ccgccgccgc
     1201 catgcggcgt gctgaacgtc ttgtgcaggt tgaaatgcat cacatcgaag ccgaggtccc
     1261 ctgggcgcgc gatcccaaga atggcgttca tgttggcgcc atccatgtag accaggccgc
     1321 ccccctcgtg gacgatgcgc gtcacttcca gcacttcgtc ctcgaagagg cccagagtgt
     1381 ttggattcgt gagcatcaac gcggcgacct gcgaatccat cgcggcgcga agctgctcta
     1441 ggtccacccg gccacggtcg ttcgagggca cgctggtgat cgtatagccg cagcgcgccg
     1501 ccgtcgccgg gttggtgccg tgcgaggagt cgggcaccag gatgcgcgtg cgcgcggtgt
     1561 cgccccgctc caggtggtag gcgcggatca tcaggaggcc ggtcagctca ccttgggcgc
     1621 cggctacggg ctgcagcgtg accgcgtcca tgccggcgat ctccgccagc atctcctgaa
     1681 gctcgtagag cagctggagc gcgccctggc tgtgttcctc gccggcgaga gggtgggccg
     1741 tcgcgaaacc cgggagcgcg gcggctgcct cgttgaccct cgggttgtac ttcatcgtgc
     1801 acgagccgag gggatagaag cccatatcca ccccgtagtt catgcgcgac agccgggtga
     1861 agtggcgaac cacctgcggc tcggagacct ccggcaacgg caggtcgccc cggatctgcc
     1921 cggcagggat gagctcctcg acggggcgct ccggcacatc acactccggg agcgagaacg
     1981 ccacccggcc aggacggctc agctcgaaga tcaacggctc ggacaaagct agctccgttc
     2041 ggtgtcgccc gcagctacgg cgaccatcgt gtcgatctgc tcgcgcgtgc gccgctccgt
     2101 gacgcacagg agcgtggcgc cctccagttc cggggagagc cggcccaggt ccagaccccc
     2161 cagaataccc gccgcgcgca accgctcatt gagcgaggcg accggctctg gcgcctttag
     2221 cgcgaattct ttgaagaagg ggcgagagaa gatcggggta aacccggcat cagcttgcag
     2281 gcgcgagtgc gcatagtgcg ccttctgcag gcacagctcg gcgacgcggc gcagcccctg
     2341 ctttccgagc gtgcagaggt acacggtcgc cgccagggcg ttgagcgcct ggttcgtgca
     2401 gatgttggac gaggcttttt cgcggcgaat gtgctgctcg cgggcctgca gcgtcaggca
     2461 gaaactgacg cggccgtcgc ggtcggtggt cgcgcctacc agccggcccg gcaggttgcg
     2521 caggaggcgg ctccggcagg ccatcaggcc caggaggggg ccaccaaagc tgagcgagtt
     2581 gcccagagac tggccctccg ccaccacgat atcggcgccg tattcgcctg ggggcgtcag
     2641 caaaccgaga ctgatcgggt cgaccgcggc gatggcaagc gcgcccaccc ggtgcgccgc
     2701 gtcgcacaac tcgcgggcat cttccaggca gccatagaaa ttggggtgct ggaagagcac
     2761 ggcggccacg cgttcatcca gctcccgttc tagtgcttct cgatccacgc cgccatcgga
     2821 tgcctgggga atctgcacca gctcgtaggg gagcggcgcg agataggtgg cgacgacgtg
     2881 ctggaagtag gggtgtatgg tgcgcggcaa gaggaagcga ttgcgtcgcg tggccgcgcc
     2941 cgccatgata gccgcttcgg cgagcgcggt ggcgccatcg tacatcgaag cgttcgccgc
     3001 gtccatgccg gtcagctggc agacgagggt ctgatactcg tagatggact ggagcaggcc
     3061 ctggctgatc tctgcctggt agggggtgta cgccgtcagg aactccgtgc gcccgacgag
     3121 cgcgggcacg accgcgggaa tgtaatggtc gtacaccccg gcgccaagga agcaacagta
     3181 ttccgccgtg cccgtggtgc gcgcggcgag atcgccgaga tgcgcaagca gctcgggctc
     3241 ggagagtgcc ggcggcagac ggagaaggcc ctgcacgcgc ccctccgggg gcacctccgc
     3301 gaagagctcc tccacgctgg atgccccgat gcgatggagc atctcccgcc ggtctgcctc
     3361 ggttccaggc aggaatcgca cgctgaacct agccttcctc ttcgacaaat gcctggtact
     3421 gctccgcggt gagcagatcc ttgacctcgc tggggtcatc catgcgcacg atgatcatcc
     3481 acccggcgga gtaggggtcc tcattcacgg tctcaggcgt atccaggagc tcctcgttca
     3541 cctcgacgac ctcgccgctg acaggggcaa gcagctccga aaccgccttg accgactcga
     3601 tggagccgaa ggtctcctcc tgctggaggg tgcgcccaac ttccggcaac tcaatataga
     3661 cgacgtcgcc gagctgatcc tgggcatagt ccgtgatgcc gatagtggca agatcgcctt
     3721 ccacgcgcac ccactcgtgg gacttcgcgt atctcagttc cggtggcaga ttcatggtta
     3781 tctccttcaa ggttccaagg tttgggaaca gcgttccacg cttcccgggc gcgagagcgc
     3841 cgggagctca acgcttccgt tggacgaaag gcagcgaggc gacgcgcgcg cggcggcgcc
     3901 tgccgcggag ttccacccag gcggctcggt ccggcaccgc ctcctcggga ggaaggtaag
     3961 ccagcgcgat ccctcggtcg agcgctggcg aaaacgttcc actggtgacg accccggtgg
     4021 cttccgtatc tgttccggcc ggtgctcctt ggcgggggat cgccttctcc tccatgacga
     4081 gccccgtgag gcggcgagtg gacggggcgg agcgggcatc tagcacggct tcgcgcccga
     4141 caaaatcgcc cttatccagg ttcaccgccc acatcagccc ggcctctacc ggattcgtct
     4201 cctcgttcac ttcgtgaccg tagagcgcgt tgcctgcctc gatccgcaga acatcgcgcg
     4261 cgcccaatcc gcaggggagc agcccgctat cgcgcccggc atccagcagg cgctcccaaa
     4321 gcgcgagggc cttcccggcc gggcagaaga attccaggcc atcctccccc gtgtagccgg
     4381 tgcgcgacgt gatcacttca gcgccgtcga agctccaccg ggcaaagccg aaacgccgga
     4441 gggtggtgaa ctcctgcccg gtggctgcgc cggcaatccc cagcgcacgc ggtccctgca
     4501 gggccaggat ggcgacctcg ccgaaatgag gggtgacctg cactccgggg gagacgtgtt
     4561 cctggatcca ctggaggtcg cggctggcgt tggcggcgtt tgccaccacc cacaggtcgt
     4621 cggacgccag cgaatagatc agcaggtcat cgagcacccc cccgttgggg gcgagcatca
     4681 agccgtagcg cacccggccc acggggagat cgcgcagggc acacgtcacc agatgctcca
     4741 gctccgcgcg cgcattggtg ccgcagagct ggaggacacc catatggctc acatcgaaga
     4801 ggccagccgc ctgccggacg gcgcgcgcct cttccaggat gcccgaatag gagacgggca
     4861 tctcccatcc cgcaaactcg accagagtgg caccgagccg gcggtgttcg gcattgagta
     4921 tggtctgttt catgagtttc cagcagccgc tggcgtcacg tgcttcagcg cctcctcatt
     4981 ggtcgtgtgg atatcgaaga tcttggagag accggtgata tcaaacacct tctgaatctg
     5041 ggggttcagg ccgaccaggc gcatggtccc accgtgctcg gccgagcgct tcaggcagcc
     5101 gaccagcgtt ccgaggccaa cgctatcgat gaagtccact ttcgacaggt tgacgatgag
     5161 cgagcgcacg ccctcctcga ccaggcgcac caccgtctct cgcagttcca acgaggtgta
     5221 ggcgtcgagc tcgccggcaa gggtgaggat ggtcgcgttg tccacacggc tgatgtcgat
     5281 ggtcaagttc atggctcctg gtctcccctc tccgtgaaac tgacttccca gaagcacggc
     5341 cctaccatgt gatcgcgcaa ctccggtgtg gcctgccccc aggcggtgcc gcgtcgcgac
     5401 gcaatggcct ggaggcactg atagcgaatc ccgccgcggt agaatcgagt gggagagcaa
     5461 gtctgaacac cgtgaggcct ccctttgcca cggccctcgc gggcgacacg caccgcttga
     5521 ttctaccaca ctacctcgct cgtgaaacgt agttcctgcc ggggcgccct cctcccgttc
     5581 gataaggcca cctctgctcc tggcggcctc catccgctcg tgccattccc acacgcgcaa
     5641 gatgcaataa cactcgtact tacaccggag cccaaccgct cgtttcctct tggagaggct
     5701 tttccctaag caaacgctcg agcccgtggc ggtagactca aacccggttc ctactccatt
     5761 ctaacacccg ggaagagggg cgtcaagagc ggcgtccggg tgttctagag gcgctgaagg
     5821 gggactgccc aactgcgggc cgggagaccg gcaggaatgc gcgcgcgctt gcggtaatcc
     5881 tcagacagca cggccgcggg accggctggc ggcacgctgc aatgtgagga gtagagatca
     5941 tgtcggacaa ggtgagaatc gcctttgttg ggtgcggggg ccatgcctcg ggggcgctct
     6001 acccgcagtt cgcgcggatt gcggagatcg acctggtggc agtggtcgac cagatcgagg
     6061 agaagcgtct ggtggcgcag cgcacctggg gcgcgcgccg cgcctatgcc agcgtcgagg
     6121 agatgctgga caaggagaag cccgaagggg tgtgcatctc gggccccccg cagatgcact
     6181 acgaggtggg cctgcaagtg ttggagcggg gcatccccat ctttgtggag aagccgtccg
     6241 cgattgactt tcagcacgcc tatgacctgg cggaggccgc gcgcaaggcc ggcaccttcg
     6301 ggatgaccgc cttcatgaag cggttcgcta ccgcctacga gaaggcaaag gagatcaccc
     6361 agcgcgagga gtttgggccg gtctcgatgg tgagcgtgcg ctacacgcat ggcgcctacc
     6421 cctcgatctg ggggatcgag gaccgcgccc tcgtctgtct gatcggcaac cagtgccacg
     6481 ccttcgatct ggcccgctac ttcggcggcg acgtgacgga gctctacgcc cgcctctgtg
     6541 atcgtggcga gggctgcttc gccttcgcag tcaccctcca gtttgagaac ggcgctgtcg
     6601 gcctgctcaa cagcaacgcc acggatgatc cgagctggaa catccgcgag acgctgaacg
     6661 tctctggcct cctctgcagc gtggatgtcg aggatatgat ccacgtgaac taccgcccgc
     6721 gcgagcgttg ggcgccggag ctgggcccct ggcgcgaggg gctgtacgcg ctgccgcacg
     6781 ccgtctcgtt caacctgggc ggacgctggc tgggctacga aggcgagttg cgccacttcg
     6841 cgcagtgcat ccgcggcgag gtggagaacc gctcgtcgct ctacgatggc gccgcctctc
     6901 tcgccatcgc cgaggcgatc tgggaaagcg ccaagaccgg caagccggtc cggcccaaat
     6961 cccacaagcc agggcagtag gtttcgcttc gcgggcgggc agggtgcggc gccgcgcctt
     7021 gcctgcccgg agctctggtc cagccggggg gagagcaggt ggggccagca aggtcctgcc
     7081 ggaggctttc ccctgccggg cagccgtggg aggagcgcgc tcgggagcgc cgaaccggta
     7141 tcgggaacga cgagggcatc cctctaccgc ctgcccggat tgccgaggaa gcgtatgctc
     7201 aaacacccgc aactcaccgc ccagcgcctc gagcgctgcc tgaacaacga gattcggccc
     7261 catatctacc aggatctcac gccgctggag gttgctgtct tccagtgtcc ggatccgatc
     7321 ccctacgccg aggcgatacg ccaggagtat cgctccgtct cggtcggctt ccgctggggg
     7381 ccggtgtggt ccacggcgtg gtttcgcttg cgcggtgcca tccccgaggc gtttgccggc
     7441 cacgaggtgg tggccctgat cgacacctcc tccgaggcgc tcgtgtggga tggcgattgt
     7501 cccggccagg gactggatgc caaccgctcc gattacctcc ttgccgagag cgccgtcgct
     7561 ggccagccgg tggagctgta cgtcgaggcc gcagggaacc acctcttcgg catctccgcg
     7621 atggggcaga ccagtggcct ggcgcgcgtg ccagagccgt ttgagctgcg gcaggcccac
     7681 ctggcgcgct tcgaccgggc gcattggaac ctcttcaacg acttccgcgt gctcctggac
     7741 ctgatgaagg ccctccccga gggcgacccg cgcaaggcgc aactcctcta cgacctgaat
     7801 gagtgtgcca accgctacct gacggggcag ggccccaagg cggcccgcga ggcgctggcc
     7861 caggcgctct cgctcccggc gcgcgcatcg agcacgcggg cgagcgccat cgggcacgcg
     7921 cacatcgacg ttgcctggct gtggcccctg cgtgagacgg tgcgcaagtg cgcgcgcacc
     7981 ttctccacgg ccctccgcta catggagcgc taccccaact acaagtt
//
LOCUS       DAOSCV010000061         8009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5857_length_8009_cov_3.736108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000061 DAOSCV010000000
VERSION     DAOSCV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_8009_cov_3.736108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLY56_02635"
     CDS             <1..586
                     /locus_tag="PLY56_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20407.1"
                     /translation="LERQEAARDVLVALGLHENPETLAMWPHPFALELEARLGERSLH
                     LDYRAQNTGSEAFAFTCALHTYFRVADIRRTALLGLEGVTRIDTLRDDIREVETRREI
                     RFDQETDFIYESAPDSVLLVDEGNARKLRIEKAGMPDYVVWNPWIEKSRRMPDFGDDE
                     YPWMLCVETGVMARPTTLRPGEQWKGTTTFRMEA"
     gene            complement(1572..2054)
                     /locus_tag="PLY56_02640"
     CDS             complement(1572..2054)
                     /locus_tag="PLY56_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ20408.1"
                     /translation="MKRKHTGFTLIQLLLAMAIIAIFACLRPTQMVGQARAVARECRL
                     SEQAFVATGRHTVWFPWTGSRIQRTLAALCRDAAGLEVREDEFTLTFEKAQPGEVRAA
                     LAGLLTSHPTAMELAAAFPIKTREKYDGYLSEELQTRLFAHNALDLDGALEVIRSAVQ
                     "
     gene            complement(2252..2327)
                     /locus_tag="PLY56_02645"
     tRNA            complement(2252..2327)
                     /locus_tag="PLY56_02645"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2292..2294),aa:Thr,seq:cgt)
     gene            complement(2485..2922)
                     /locus_tag="PLY56_02650"
     CDS             complement(2485..2922)
                     /locus_tag="PLY56_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20409.1"
                     /translation="MADGERPEDQGQRPAEVVRTVFERLGETLDVTAKTARLSLDIGA
                     LNARKGGIFQEMGRKVYELYGKGLVKNSALLALCADVAEIDSQIAEKRAQIEELRGKS
                     GKEAVIEEEELGIDEEPAAGPTTSDDLSDEEEREVRPSTDKNL"
     gene            complement(2956..3588)
                     /locus_tag="PLY56_02655"
     CDS             complement(2956..3588)
                     /locus_tag="PLY56_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20410.1"
                     /translation="MGVVRTGEDAPEVCPRCRRPTRRDFHFCPTCGERLQEVCSACRA
                     PLEKDWAFCANCGTHREARAVLFAAAEPMREQEGGTLPTEEEGLASVISERAEEHNSR
                     GSELYENDEFGEAVREFSAAVSLVPTHPVYRTNLAVALSEMGEYERAVSEFLEALRLD
                     PDNAGAYLQLGYTYQEMERPQDAMNVWRRVVELAPDSAEAEEARDAMESI"
     gene            complement(4021..4668)
                     /locus_tag="PLY56_02660"
     CDS             complement(4021..4668)
                     /locus_tag="PLY56_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20411.1"
                     /translation="MKQRSGFTLIELLVVIAIIAILAAILFPVFARARENARKSSCMS
                     NVKQLAIGVTMYLQDYDEMFFIRRQEPGGTPTICWYPLSSQPTYLLEPYVKNAKIFQC
                     PSMTNLAIGYGYNLCLPGVTMSRVEEPSELVVLGDDQFGSGWYYAPSQGTSLFGANFC
                     KTPGTATAGVQWGVNAPYGRHSDGVNIAFADGHAKWIKPEKLWNGGNDKPHYKQW"
     gene            complement(4852..5538)
                     /locus_tag="PLY56_02665"
     CDS             complement(4852..5538)
                     /locus_tag="PLY56_02665"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HOJ20412.1"
                     /translation="MGFRQILIFFLASVVVATGCTPGERQVEQAIQRELPRLVGPAAS
                     YHVDIQGLQGARSAERVTATGRRVRPEGSPVLDHVAVELNGVTYDTERGQLERVENAE
                     VIARVQAADLAAFLEAQRTLRDVTVDLEPPARVRVRARPEIPGLEIPPGVLIDAEGRL
                     VGEGARVRYDIDRVHAAGATLPGGVVVRLSNAINPVVDLSRIPVILRVVELRVEGESM
                     VIRATGRYPQ"
     gene            5936..6220
                     /gene="groES"
                     /locus_tag="PLY56_02670"
     CDS             5936..6220
                     /gene="groES"
                     /locus_tag="PLY56_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ20413.1"
                     /translation="MLRPLRDRVVIKPSEQEEKTAGGILLPDTAKKKPQEGEVVAVGS
                     GKVLEDGTRTPMNVKVGDRVVYSKYAGTEVRVGDEEYLVIDEDSIYAIKE"
     gene            6261..7886
                     /gene="groL"
                     /locus_tag="PLY56_02675"
     CDS             6261..7886
                     /gene="groL"
                     /locus_tag="PLY56_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049565.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ20414.1"
                     /translation="MAAKMIIYDEDARRALERGANLVAQAVKVTLGPKGRNVVLDKKW
                     GAPTITKDGVTVAKEIELKDPYENMGAQLVKEVASKTNDVAGDGTTTATVLAQAIVNE
                     GLRVVAAGANPMAVKKGIERAVEKVVEEIKSRAIQVTDRASVANVAAISGNDREIGDL
                     IAEAMEKVGKDGVITVEESKGTATTLEFTEGMQFDKGYISPYFVTDPERMEAVYEDPL
                     ILIHEKKISAAADLIPLLEKISAARRPLLIIAEDVDGDALATLVVNKIRGTLNVVAVK
                     APGFGDRRKAMLEDIAILTDGRFLSEDLGVKLENVDLSMLGKAKRVTITKENTTIVEG
                     AGSQEAIQGRIAQIKRAIEETDSSYDREKLQERLAKLAGGVAVIKVGAATETELKEKK
                     HRFEDALSATRAAVEEGVVAGGGATLVNVIPCLDSIEAEGDEKTGVNIVRRALEEPLR
                     TIANNAGLEGSVVVERVKSEKPGIGLNALTEEYVDMVQAGIIDPAKVARSAVQNAASI
                     AGMILTTETLVAEKPQKEEAAPAGGGGYGDYDM"
BASE COUNT         1498 a         2589 c         2516 g         1406 t
ORIGIN      
        1 ccttgagcgc caagaagcgg cgcgggatgt tttggtggcg ctcggcctgc acgagaaccc
       61 ggagacgctg gcgatgtggc cgcatccgtt tgccctggag ctggaggcac gcctgggaga
      121 gcgctcgctc catctggact accgcgcgca gaacacgggt agcgaggcgt tcgcgttcac
      181 ctgcgccctg cacacctatt tccgcgtggc cgacatccgg cggacggcgc tcctgggcct
      241 ggaaggcgtg acgcgcatcg acacgctgcg cgacgacatc cgggaagtgg agacacgccg
      301 ggagatccgg ttcgaccagg agaccgactt catctacgag agcgcgccgg atagcgtctt
      361 gctcgtcgac gaagggaacg cccggaagct ccgaatcgag aaggccggca tgccggatta
      421 cgtcgtttgg aacccgtgga ttgagaagtc gcggcgcatg cccgacttcg gcgacgacga
      481 gtatccgtgg atgctctgcg tggagaccgg cgtgatggcc cgcccgacca ccctccgccc
      541 cggcgagcag tggaagggca caaccacatt ccgcatggag gcttgatcta aggggggaat
      601 tatccgcaga tggcgcagat tatcgcagat agaatgaacc ttcctgccat gccagtgcag
      661 ccccgcccgc gcccggcata acccaaagcc cggtcgggtt tcattccatt tgtgttctct
      721 gcgccatttg cggataatag ttccatgtgg tttcttggga atcatagtca cctggaatag
      781 gagcgagcgg caggtaagcc acaccctcgg tgcccgctcc aggaatcggg tatgctgccg
      841 tacgagtgcg tatgcccgcg tgtggcacct gtggaatatg tcgatgctat cgacatatcg
      901 ctcgaatatg tcgattttct tccgtttttt cgacatatta gcagtcgggc gtggcgaaac
      961 tggaagctca cgaatggcgc aggcaataac cgcgtgcgct ctctgagtgg ccgccgagcg
     1021 ccattgtcga taatggcgct ctccgcgcat ccgggatgtt gcaatccctc gtagaaggag
     1081 atagctacag ggccttgccg gacaccgggt tgagccgcaa caggggccat catgcgggaa
     1141 gtcgacggtg ctcagttgtc ggtggctcca gacaccgttc ggctggcgac atccgtgcat
     1201 taggcatttg tgaccggata ccggtctttc cagcgcccgc agggagaatt acagggccgc
     1261 gatggcgtct catggcaatc cgtgtcgtgt gtgtccacat cttcacgatc cggggcaact
     1321 catcgagaga ccctcgctgt gatgcgaaag gactatcgct ctattcatga ccgctgggct
     1381 cccccatcgc ccggtcgtgg tgctgtgccc gcatggagaa caggccggtt gcggcgtcgc
     1441 tcatgtgctt cagcgcgccc gcgaaaccgt gcgcgttgcc ggtccgggtg ttccacggcg
     1501 cgccaagcgt gttctattcc gaccatcccc gctcaccgtc cgggtggccg gtatccgtgc
     1561 gcccgtgcag gctattgaac tgcggaccgg atcacctcga gcgcaccgtc caagtcgagc
     1621 gcgttgtgcg cgaagagccg cgtctgtagc tcttctgaga gatagccgtc gtacttctcg
     1681 cgtgtcttta tcgggaaggc ggccgcgagc tccatagccg tcgggtgcga ggtcaggagc
     1741 ccggccagcg cggcccgcac ttcgccaggc tgcgctttct cgaaggtcag cgtgaactcg
     1801 tcttcgcgca cctcgagccc ggccgcgtcc cggcacagcg cggccagggt gcgctggatg
     1861 cggctgcctg tccaggggaa ccacaccgtg tggcggccgg ttgccacgaa agcctgctcc
     1921 gacaggcggc actccctggc gactgcgcgc gcctggccca ccatctgcgt cgggcgcagg
     1981 cacgcgaaga tcgcgatgat tgccattgct aacaggagtt ggatcaaggt gaagccggta
     2041 tgctttcgct tcatgccagg cgccctctct cgagcatccc cgtattgtgc cagtcgcgct
     2101 gccggattat cccacaaagc cgttctgatg tgcagtctca cgaagaagcc cgggaagagg
     2161 caacaggagg gggatgatgc acaaatggcg cagatgagca caggtaacct gtgctcatct
     2221 gcgccatcta tggataacgg ttggaaatcc gtggagccga cgaagggagt cgaacccttg
     2281 acccgcgcat tacgaatgcg ctgctctggc cactgagcta cgtcggcatc gcactccaag
     2341 caagcagatt atagcatcct accgagatcg tgtcaagatc gattccgcgg cttttctcca
     2401 cggaacgggc aggccggagg tgccgtcccc aacgcttgcc cagggatacc gcgcgcccac
     2461 atgcttagac cagtggtctc ctgctcagag attcttgtct gtggaaggtc ggacctcgcg
     2521 ctcctcttca tcgctcaggt cgtcgctcgt cgtcggacca gctgccggct cctcatcgat
     2581 gcccagttct tcttcctcga tcaccgcttc cttgccgctc ttcccacgca actcctcgat
     2641 ctgggcgcgc ttttcggcaa tctgcgagtc gatctccgcg acatcggcgc acagcgccag
     2701 gagcgccgag ttcttgacca ggcccttgcc atacagttca tagaccttgc gccccatctc
     2761 ttgaaagatg ccccccttgc gggcgttgag ggcgccaatg tctaggctca accgcgccgt
     2821 cttggcagtc acgtcgagcg tctcgcccag gcgctcgaac accgtacgaa caacctccgc
     2881 ggggcgctgt ccctgatcct ccggcctctc tccgtcagcc atctttccct ccgcgatccg
     2941 ccgctcgccg cgggctcaga tgctctccat cgcatcgcgc gcttcctccg cttccgcgga
     3001 atctggagcc agctccacca cgcgacgcca cacattcatc gcatcctgcg ggcgctccat
     3061 ctcctggtag gtgtagccca gctgtaggta agcgcccgcg ttgtcgggat ccaggcgcag
     3121 cgcctcgaga aactccgaga cggcgcgctc gtactcgccc atctcgctca gggcgacggc
     3181 cagattcgtg cggtaaacgg gatgcgtggg caccagggag accgcggcgc tgaactcgcg
     3241 caccgcctct ccgaactcat cgttctcata cagctcggag ccccgggagt tgtgctcctc
     3301 ggcgcgctcg ctgatcacag acgccagccc ctcctcctcg gtggggaggg tgccgccctc
     3361 ctgctctctc atcggctcgg cggcagcgaa gagcacggcc cgcgcttcac ggtgcgtgcc
     3421 gcagttggcg cagaaagccc aatccttctc cagcggcgcg cggcaggcgc tgcagacctc
     3481 ctgcagtcgc tcgccacacg ttgggcagaa gtggaagtcg cgccgcgtag gacgacgaca
     3541 gcgcgggcag acctcgggcg cgtcttcccc ggtgcgaacc acacccatca gcaatgcctc
     3601 ctaatccgag cgggagacaa acgactgttc cccaacacat ccgctcctct ggcggacgcg
     3661 tctcattata cgttgggaga gggtttagtg tcaacgaaat ggatctcttc cgggggcttc
     3721 cgcgatgacc gcgcggtagc tcctctcccc tggggccggc gcggggaaat cagcagtact
     3781 acggatgaag ggacggacat ggatggagag ggtgcgcacg cccggagttg ggtgccccat
     3841 tcgggggacc accacgcggc agctctcctt catccgaggg gtcgtcgcgg gacaagaggg
     3901 tagggacgag gctatgggtt aggcgagtcg ccggcagcga tgcggcggat gggggaagcg
     3961 cgcgcaccca caagacatgc agcgcgggcc gaacacccgc gctgcctgac atctctcacc
     4021 ttaccactgc ttgtagtgag gcttgtcgtt accgccgttc cacagcttct cgggcttgat
     4081 ccacttggcg tggccgtccg cgaaggcgat gttcacgcca tccgaatgcc ggccatacgg
     4141 cgcgttcact ccccactgaa cacccgcggt ggccgtaccc ggcgtcttgc agaagttggc
     4201 gccgaagaga ctcgtgcctt ggctcggcgc gtaataccat ccggagccaa actggtcgtc
     4261 gcccaggacc acgagctccg atggctcctc caccctcgac atagtcacac ccggaaggca
     4321 gaggttgtag ccgtacccga tggcaaggtt ggtcatcgag ggacactgga agatcttggc
     4381 gttcttcacg tacggctcta acaggtaggt gggctgcgag ctgagcggat accagcagat
     4441 cgtgggcgtt ccaccaggct cctgacggcg gatgaagaac atctcgtcgt agtcctgcag
     4501 gtacatcgtg acgccgatcg ccagctgctt cacgttggac atgcaggagc tcttgcgcgc
     4561 gttttcacga gcacgcgcga atactgggaa gaggattgct gccagtatag caatgattgc
     4621 tatgacgaca agcagctcga taagtgtaaa cccggatcgc tgcttcattt gggttctcct
     4681 tcggtcttgg tggaaccgag ggcagatcgc ctcggcctat ggaggtatta taacaagagc
     4741 ctacgctgcc gtcaatgcag cgcgcgcccg attcatgctg cgatagcacg gcagcctcgc
     4801 gcatccccag gggctcagac aaacccagat cgcatggaat acggcgatga gctactgcgg
     4861 atagcgtccg gtggcgcgga tcaccatgct ctccccttcg acgcggagct ccaccactcg
     4921 gaggatcaca ggaatccgag aaaggtccac cacggggttg atcgcgttgg agagccggac
     4981 gaccactccg ccgggcagag tcgcccctgc ggcatgcacc cgatcgatgt cgtagcgtac
     5041 ccgcgcgccc tctccgacga ggcggccctc ggcgtcgatg agcacgcccg gcgggatctc
     5101 caacccgggg atctccggcc gtgcccgcac acgaacgcga gcgggcggtt cgagatccac
     5161 ggtcacatct cgcagggtgc gctgcgcctc cagaaaggca gcaaggtccg ccgcctgcac
     5221 ccgtgcgatc acctcggcgt tctccacacg ctcgagctgg ccgcgctccg tatcataagt
     5281 gacgccgttc agttcgactg cgacgtgatc aagcacgggc gagccttcgg ggcgaactcg
     5341 ccggccagtg gcagtcaccc gctctgcgct gcgcgcgccc tgcagcccct gaatatccac
     5401 gtggtaactc gcggctgggc caaccaggcg cggcagctcc cgctgaatgg cttgctcaac
     5461 ttggcgctcg cccggggtac acccggtggc aaccacgacg ctggcaagaa aaaagatgag
     5521 gatctggcga aagcccatgc cgacctcctc gcgccaaagc ctccggcgcc gtcccggcgg
     5581 acgttaccca tccaaacggg ccatgaaacg cccccgccac gcgctggtgc aacctaacag
     5641 tgtccgcccc ccgcgctggc aaccgggccc tttccggctt gcaagacgcg cgcgcaggca
     5701 ccctgacgcc tgtctatgga ccctcaccga aaggcgctgt tgatccgtgc gacgcccctt
     5761 gacaaagcct ttgcctttcg gtaaaatagc ggcaacatta gcactcgccc cccgtgagtg
     5821 ctaatcccgc ccccgagtgg acgggatcga cagggctatt ttggttcccg ccgcgtgcag
     5881 gcggcgagcg gaacaggctc tcacaggttt ctcgcgaagg gaggtgtact ctcaaatgct
     5941 tcgacctctg cgtgaccgtg tggtcatcaa gccctccgag caggaggaga agacggcagg
     6001 tggcatcttg ctgccggata ccgccaagaa gaagccccag gaaggggaag ttgtcgccgt
     6061 gggcagcggt aaggtgctgg aagacggaac gcgcaccccg atgaacgtga aggtgggaga
     6121 tcgcgtggtc tactccaagt acgctggcac ggaggttcgc gtcggcgatg aggagtacct
     6181 cgtgattgac gaagactcca tctacgcgat caaggagtga tgggacaccc ccaacgatac
     6241 cctcagggag gttttgacgt atggcagcca agatgatcat ctatgatgag gacgctcgcc
     6301 gagcccttga gcgcggagcc aaccttgtgg ctcaggcggt caaggtgacg ttgggcccca
     6361 aaggccgcaa cgtcgtcctc gacaagaagt ggggcgcgcc cacgatcacg aaggacggcg
     6421 tgacggtcgc caaggagatc gagctgaagg atccctacga gaatatgggt gcccagctcg
     6481 tgaaggaagt cgcctcgaag acgaacgacg tcgcgggaga cggtaccacc accgcgaccg
     6541 tgttggcgca ggcgatcgtg aatgagggtc tgcgcgtcgt cgccgccggc gccaacccga
     6601 tggccgtgaa gaagggcatc gagcgcgccg tcgaaaaggt cgtcgaggag atcaagagcc
     6661 gggcgatcca ggtcaccgac cgcgcctccg tggcgaacgt cgccgccatc tccggcaacg
     6721 accgcgagat cggcgatctc atcgccgaag cgatggagaa ggtcggcaag gacggcgtca
     6781 tcaccgttga ggagagcaag ggcacggcta ctaccctcga gttcaccgag ggcatgcagt
     6841 ttgataaggg ctacatctcc ccttacttcg tcaccgaccc tgagcgcatg gaggcggttt
     6901 acgaggatcc gctcatcctg attcatgaga agaagatctc ggctgctgcg gacctcatcc
     6961 cgctgttgga gaagatctcc gccgcgcgtc gcccgctgct gatcatcgcg gaggacgtgg
     7021 acggcgatgc actggccacc ctcgtggtca ataagatccg cggcaccctc aacgtggttg
     7081 ccgtgaaggc ccctggtttc ggcgatcgcc gcaaggcgat gctcgaggat atcgccatcc
     7141 tcaccgacgg tcgcttcctc tccgaggatc tcggcgtgaa gctcgagaac gtggacctct
     7201 ccatgctcgg caaggcgaag cgcgtcacga tcaccaagga gaacacgacc atcgtcgagg
     7261 gcgcgggcag ccaggaagcg atccagggcc gcatcgccca gatcaagcgc gccatcgagg
     7321 agaccgattc ctcctacgac cgcgagaagc tccaggagcg cctggcgaag ctcgcgggcg
     7381 gcgtggccgt gatcaaggtc ggcgcggcga ccgagacgga gctcaaggag aagaagcacc
     7441 gcttcgagga cgccctcagc gccacccgcg cagccgtcga ggaaggcgtg gtggccggcg
     7501 gcggcgccac gctcgtgaac gtcatcccct gcctcgatag cattgaggcc gagggcgacg
     7561 agaagacggg cgtgaacatc gtccgccgcg cgctggagga gcccctccgc accatcgcca
     7621 acaacgccgg cctcgagggc agcgttgttg tcgagcgcgt caagagcgag aagccgggca
     7681 tcggcctgaa cgccctcacc gaggagtacg tcgacatggt gcaggccggg atcatcgacc
     7741 cggcgaaggt cgcgcgctcc gccgtgcaga acgcggcctc catcgccggc atgatcctta
     7801 ccaccgagac ccttgtggcc gagaagccgc agaaggaaga agccgcgccg gccggtggcg
     7861 gcggctatgg cgattacgac atgtagcgag gacgagggcc ggcgatatta gccggagatc
     7921 gcccttcgtt tcagtaaccg cgcttgcggg ccgggacggg attagccgtc ctggcccgcg
     7981 cctctctgca ccctctccca cctcgacgc
//
LOCUS       DAOSCV010000062         7898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5948_length_7898_cov_3.307025, whole genome shotgun sequence.
ACCESSION   DAOSCV010000062 DAOSCV010000000
VERSION     DAOSCV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5948_length_7898_cov_3.307025"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..627)
                     /locus_tag="PLY56_02680"
     CDS             complement(133..627)
                     /locus_tag="PLY56_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ20415.1"
                     /translation="MKRMMRNPEGGFSLVELLAVVLILAVLAAVAVPLYINTRRSAAA
                     RACKGNLAAIAAAASAFALRNGEYPTAANVLSAYNPTNPTAGGLVGAPEGLSSAMACP
                     LDNAAYTFTGGGTSALVITCPHASTAAGGDNAHETVVPSPTGTEAISAHWRPPQRTTC
                     PSTA"
     gene            complement(934..2145)
                     /locus_tag="PLY56_02685"
     CDS             complement(934..2145)
                     /locus_tag="PLY56_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ20416.1"
                     /translation="MPVYQYRARDAQGQLVTEVLAYEDELSLRRYLRENDLFVLGISE
                     VKRSVFRNPFQRGVGLQDLIIMTRQLRTMIHAGMPLYTGLETLAEQTPNPKLAEVLTE
                     VARAVAHGVSISSAMAQYPRYFPPMLLTLIQAGEEGGRLPETLNEAARQLELLMEIRQ
                     KLISALVYPAFTFLATVGTVAAMLIWIVPVFAQIYEELNAPLPGITRLLIAISDLVVH
                     NGWIMLLALIAGLDALRRYYRTPRGRLRIDGLKIRIPLLGQLFLKSATANLTGSLAGL
                     LRSGVGLLPALQTAAQVCGNEVLAEAARTAAKNVSVGRRLSEELEASGQFPAMVIRMV
                     AVAEDLGTLPDVLQEITVSYNEDVEYTIRRMMALVEPVTVLCLGGVVGFVLVALYYPI
                     FNMGNVFLSGA"
     gene            complement(2149..3879)
                     /locus_tag="PLY56_02690"
     CDS             complement(2149..3879)
                     /locus_tag="PLY56_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ20417.1"
                     /translation="MMRANTTVIERLFRPAPDRRLGERLVQEGHITEQQLRQALEVQG
                     RTHGLLGQILVDLGFVSAPTVGRLMAKNFGVEYVDLMEVRPDPSAVHLVPESVIRAMQ
                     VLPLRCVGDTIEVAMIDPLNVAAIDTIHQHTNLRVVPYLTMAAELQRAINDQFDAHTR
                     ASEALQQLESEDNEQSGLSHAELVAASEAPIVRLVDSIIESALALRASDIHFEPHEGG
                     LRVRFRVDGTLLDQTDIPRSQRAPLTARLKVLCLMDITENRRPQDGRMTYNEHGRIYD
                     IRASSVPTVFGEKIVLRILDKSSVMVPLGKLGFQGDQQRRFEGLIRQPHGMVIVVGPT
                     GSGKSTTLYASLNLLNDSTRNIMTLEDPVEYHVQGINQVQVNPRIGLTFASGLRAFVR
                     QDPDVILVGEIRDEETADMAVQASLTGHLVLSTLHTNSAVGTVARLTNLGVNPFLIAQ
                     SLLGVCSQRLVARICNHCAVEYQPAPDVLESVGLHPDEARGITFRRGTGCRTCHGRGY
                     LGRIGLYEVLTVNEEIRLLIMRGASEEEIQAAAERNGMMSLRDCAAAAVKAGITTPEE
                     MGRVVLAKGG"
     gene            4451..6043
                     /locus_tag="PLY56_02695"
     CDS             4451..6043
                     /locus_tag="PLY56_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20418.1"
                     /translation="MRVKQVILGMAILLATHGTQALAAPDALTEGPGNDTEAAWSPDG
                     RQVVFQSDRNGDLDLYVMDLASRQVRPLVTGPGHACFPAWSPDGQWIVYAFAHFTKTA
                     VQGIEDGYNLFVVPAAGGEPRRLTRGPFRDYTPTFSRDGRSIYFSSTRGAEQSSVGLY
                     RVPFEGGEPERVLHHDGPDVALVQPDLSPDGRLIAVGHIAGFRGNWSVRLLRTATPDA
                     EFALTGPDAPLYSPRWSPDGRLIACTGYRVGDPGWGIYLVELRTGSAVRLDTGPGNSR
                     SPAWSPDGKTLLFENNRTGSYKLYRMEVPRVSFPPVKAEAIRTDPPVFQLALKESTGT
                     EVKDASALGNHGRVLGTVGREEGALVFTGQGSITVPQPKGFDFGTGTFAVRVVLTVEK
                     HTDALRIVAVGDYPTHHLGWQIYLGPDNRAWFNARDPGGLFVGAASDRPVPVGRKVTL
                     VGIRHASGRVELHLDGRVQASAGAGAVMSYPVPTQVRIGSQYDGASPFAGRLHEMAVY
                     RRPLTGREGGAPTLAEFLGEAK"
     gene            6043..>7898
                     /locus_tag="PLY56_02700"
     CDS             6043..>7898
                     /locus_tag="PLY56_02700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ20419.1"
                     /translation="MEARASASIGRVARAAACAVGILLSGVAADGAERFEVAAWVDHF
                     DFAGVLQEDRYLFDSETAEGCARILDHVQEVGATTVLWRNCGGGTMRYQSRVDSHHND
                     GILDKRRVLGNRPVWDWVRYGDADPDLMRTALRLCKERGLRPGVHWPFEETHWQIWTI
                     GRFNLENPQLWGRTADGRPWWGRCSLAHEEVIRHKLALVDELVERGMEVLFIDFFRTG
                     GWSPAYEYVEPVVKAYREQYGTEPPVDHRDPRWKRHVSRYVTEFLRQVRGRLNASGRK
                     IELAVGIPGIAPLATSDTLAFGADWRAWVEEGLIDTLVINTLHWDAKDPMGSTRALGR
                     EVMQAVNGRCRVLWPVRAYDYSGYGIPSYRKALPQMTDAQIAAQLTEMAWEEGAAGIS
                     LECVDYNNYAPETRKAMRALLRDNRFDVINTHSSTDSWLAALACKMLADAPPIVRTRH
                     LSTAVNRSWSTRWLYEKGCAHVVVTGEALKAQLIRDNGFPAAQLTSVPTGIDLERFHP
                     QPRASARAALGLSAEATYIGILATLRDWKGHTVLFDALTQLLPSRPNLRVLIIGDGPY
                     RDRLDTRAEVLGITDRLDFVGHRTDAERWLPVIDVFTLPSWGDEGVSQALMQA"
BASE COUNT         1314 a         2478 c         2700 g         1406 t
ORIGIN      
        1 cagagtgctg tggggccatg ctcgaggggt atcgtccctg tctcaccgcg catctctcca
       61 tcaaacggct ccttcgggtg cctggtggca aagaagcctg ccgggccccg agggacccgg
      121 caggcttggg ggttacgccg tgctagggca ggttgtccgc tgcgggggcc gccagtgtgc
      181 gctgatagcc tccgttccgg tcgggcttgg caccactgtc tcgtgcgcgt tatcgccacc
      241 tgccgccgtg ctggcgtggg ggcaggtgat gaccagcgcc gacgtgccac cgcctgtgaa
      301 ggtgtaggcc gcgttatcca gcgggcaagc catggccgac gacagaccct cgggcgcacc
      361 cacgaggcca ccggcggtgg ggttcgtcgg gttgtaggcc gacagaacgt tcgccgccgt
      421 gggatactcg ccgttgcgga gggcgaaggc cgaagccgcc gccgcgatgg ccgccaggtt
      481 gcccttgcat gcacgggcag ctgccgatcg gcgtgtgttg atgtagagcg gaacggccac
      541 agcggccagt accgccaaga tcagcacgac tgcaagcagc tcaacgagag agaaaccacc
      601 ttctgggttg cgcatcatcc gcttcatcgt ttactcctcc tcctgcaata ccactgcatc
      661 ggactgcggc cgggcccggg ccgctcgggt ccggtgggta gagcaccgga actcttctgg
      721 ctcctcggct gcggggtggc ttcggaggcc actcctactt ctcgggtggc ggcggcttcg
      781 ccgctccggc tcgtgtcttc agaccgggtg cgtctcgctc gcttcctcgt cgagacgcct
      841 catgataggt cactccttcg ataggtcgga tgcctcgttg aggcgggctc cacaaatcgc
      901 ccgccatcta ttcctgtgca gatctcgtgc caattacgct cccgagagga acacattgcc
      961 catgttgaag atggggtagt agagcgccac cagcacgaag cccacgacgc cgcccaggca
     1021 gagaaccgtg accggctcga cgagcgccat catgcgccga atcgtgtact ctacgtcctc
     1081 gttgtaggag acggtgatct cttgcagcac atctggcagg gtgcccaggt cctcggcgac
     1141 ggccaccatg cggatcacca tggcggggaa ctgcccgctg gcctccagct cctcggagag
     1201 gcgccggcct acgctcacat tcttggcggc ggtacgcgcg gcttcggcga gcacctcgtt
     1261 gccgcacacc tgcgctgcgg tttgcagcgc cggaagaagc ccaacgccac tgcgcaacag
     1321 gccggccagg cttcccgtca ggttggccgt ggccgatttc aggaagagct gccccagcaa
     1381 gggaatgcgg atcttcaagc catcgatccg caggcgtcct ctgggagtgc ggtagtagcg
     1441 gcgcagggca tcgaggcctg cgatcagagc cagaagcatg atccagccgt tgtgcaccac
     1501 caaatcgctg atcgcgatga gcagccgcgt gatcccgggg aggggcgcat tcagctcctc
     1561 gtagatctgg gcgaagaccg gcacgatcca gatcagcatc gccgccaccg tgccgacggt
     1621 ggccaggaaa gtgaacgccg gatagacgag ggcactgatc agcttctgcc gaatctccat
     1681 cagcaactcg agctgacgcg cggcctcgtt gagagtctcc gggagccgcc cgccctcctc
     1741 gccggcctgg attaaggtga gcagcattgg cgggaagtag cgcgggtatt gcgccatcgc
     1801 gctcgagatg ctcacgccgt gcgcaaccgc tcgcgcgacc tcggtcaaca cttccgcaag
     1861 cttggggttg ggggtctgct ccgcgagcgt ctccaggccg gtgtagaggg gcatccccgc
     1921 gtggatcatc gtgcggagct ggcgggtcat gataatcaag tcttgcaggc cgacgccccg
     1981 ttggaatggg ttgcggaaca ccgagcgctt gacctcggat attcccagaa caaagaggtc
     2041 gttctccctc aagtagcgac gcagcgagag ctcatcctcg taggcgagga cctcggtgac
     2101 cagctggccc tgcgcgtcgc gggcccgata ctggtagaca ggcatctgtc agcctccttt
     2161 cgccagcacc actctgccca tctcctcggg ggtggtgatg ccagccttca ctgccgccgc
     2221 cgcgcagtcg cgcagcgaca tcatgccgtt ccgctccgca gcggcctgga tctcctcttc
     2281 cgaggcgccg cgcatgatga gcagccggat ctcctcgttg accgtcagca cttcgtagag
     2341 cccaatgcgg cccagatagc cgcgcccgtg gcaggtgcgg caaccggtgc cccgacggaa
     2401 tgtgataccg cgcgcttcat cggggtggag accgacggat tcgagcacgt ctggcgccgg
     2461 ctggtattcc accgcgcagt ggttgcagat tcgggccacc agacgctgag aacagacgcc
     2521 gagcagcgat tgcgcgatca ggaaggggtt cacgcccagg ttggtcagac gggctacggt
     2581 gccaacggcg ctattggtgt gaagcgtgga gaggaccagg tggccggtaa gcgaggcttg
     2641 caccgccatg tccgccgtct cctcatcgcg aatctcgccg acgaggatga catccggatc
     2701 ctggcgcacg aaggcgcgca ggccgctggc gaaggtgagt ccgatgcgcg ggttcacctg
     2761 cacctggttg atcccctgga catggtactc gaccgggtct tccagggtca tgatgttgcg
     2821 cgtggagtcg ttgagcaggt tgagcgaggc atagagcgtg gtgctcttgc cactcccagt
     2881 tggccctacg acgatgacca tgccatgcgg ctgccggatc aggccctcga accggcgctg
     2941 ctgatcgccc tgaaagccca gcttgccgag cggaaccatg acggaggact tgtccaagat
     3001 gcgcaggacg atcttctccc cgaagacggt cggcacggac gaggcacgga tatcataaat
     3061 gcggccgtgc tcgttgtagg tcatgcgtcc gtcttgcggc cggcggttct ccgtgatatc
     3121 catcaggcag aggaccttca gccgggcagt gagcggcgcg cgctgggacc ggggaatatc
     3181 ggtctggtcc aggagtgttc catccacgcg gaaacggacg cggaggcctc cctcatgtgg
     3241 ctcgaagtgg atatcgcttg cgcgcagcgc gagtgcgctc tcgatgatcg aatcgaccag
     3301 gcggacgatg ggcgcttcgc ttgcggccac cagctccgcg tgcgagaggc cggattgctc
     3361 gttatcctca ctctcgagct gctgcagcgc ctcggaggcg cgggtgtggg catcaaattg
     3421 gtcgttgatc gcccgctgca gctccgcggc catcgtcagg tagggcacga cgcgcaggtt
     3481 cgtatgctgg tggatggtgt cgatcgccgc cacgttgagc ggatcgatca tggccacctc
     3541 aatggtatcg ccgacacagc ggagcggcag cacttgcatg gcgcggatga cgctctccgg
     3601 caccagatgc accgccgagg gatccgggcg tacctccatc aggtcgacat actcgacgcc
     3661 gaagttcttg gccatcagcc ggccaactgt aggcgcgctg acgaacccca ggtccacgag
     3721 gatttgaccc aggagaccgt gggtgcgtcc ctgcacctcg agggcctgac gcagttgttg
     3781 ctcagtgatg tggccctctt gcaccaggcg ttctcccagg cgacgatcgg gtgctggccg
     3841 gaagagccgc tcaatgaccg ttgtgtttgc tctcatcatc tatgccatct atccttcgcg
     3901 tggagtccac cccgacgggg cgggatagac ccactggcca gaatccggcg acccatgccg
     3961 ccggacccct cacgggcaag ccctgcctac gctgccgggg tatccgaccg cgtacgccgc
     4021 gcgccgatac gttgcgtgtg tcgaaaagag tatcgcggtg ccagctcgcg acgggtgggc
     4081 gggcccgccg attgccggaa ctctcgctcg ggcaagtggg ggtgtgcaaa aggaatgcca
     4141 tttcccgcac ggggttggat ctataggggc ttgctaagga ccccgtcctg ggaaggagtg
     4201 gcctgccagg agcccgaaga ggggggccaa agaaacgctt gccgctgatc gtgggatggt
     4261 accgcttgat ggtggcacac ccgggcaatc aacctggaga gccggcgctc tccaggggat
     4321 gcgcgcacgg ttgcggcgaa ctgcctcgga atgctcgctt cagctggtgt ggttcaaacg
     4381 cgagaagcgg gcactccaca ctggcgccga gagtggcggc attctcgacg caagccgtaa
     4441 ggagctgcgt atgagggtga aacaggtgat cttggggatg gctatcctgc tcgcgacgca
     4501 tggcacccag gcgctcgcgg cgcccgatgc cctcaccgag gggccgggca acgacacgga
     4561 ggcggcatgg tcgcccgatg gccggcaggt ggtcttccaa tccgaccgga acggcgacct
     4621 ggatctctac gtgatggatc tggcgagccg ccaggtgcgc cccctggtta ccgggcctgg
     4681 gcacgcctgc ttcccggcgt ggtcgcccga tggccagtgg atcgtctatg cctttgccca
     4741 cttcacgaag acggcggtgc aaggcattga ggacggctac aacctcttcg tagtgcctgc
     4801 tgcgggaggg gagccccgcc ggctgacgcg cgggccgttt cgcgactaca cgcctacgtt
     4861 cagtcgggat gggcggtcga tctacttctc ctcgacccgg ggcgcggagc agagcagcgt
     4921 tggcctctac cgcgtgccat tcgagggcgg cgagccggag cgggtcctgc atcatgacgg
     4981 ccccgatgtc gctctcgtgc agccagacct ctctcccgat ggccgattga ttgccgtcgg
     5041 ccacatcgcc ggcttccggg gcaactggtc ggtgcgcttg ctgcggacgg cgacgccgga
     5101 tgcagagttt gcgctcacgg ggccggatgc accgctctac agcccgcgct ggtcgcccga
     5161 tgggcgtctg atcgcctgca ccggctaccg ggtgggcgat cccggctggg gaatctacct
     5221 ggtggaactg cgcacgggaa gcgccgttcg tctcgacacg ggtccgggca actcgcgaag
     5281 ccccgcgtgg tctccggatg gcaagactct tctctttgag aacaaccgga ccggcagcta
     5341 caagctgtat cggatggagg tgccccgcgt gagcttcccg ccggtgaagg cagaggcaat
     5401 ccgcacggat ccccctgtat ttcaactcgc cctcaaagag agcacgggca ccgaggtgaa
     5461 ggatgcgagc gccctgggca atcacggtcg ggtgctcgga accgtgggcc gcgaggaggg
     5521 tgccctggtc ttcaccggcc agggctcgat caccgtgccg cagccgaagg gcttcgattt
     5581 cggcacgggc actttcgcgg tgcgcgtggt cttgaccgtc gagaagcaca cggacgcgct
     5641 gcggatcgtg gcggtgggtg actaccccac gcatcacctg ggctggcaga tctacctggg
     5701 cccggacaac cgtgcctggt tcaacgcgcg cgatccgggt ggtctttttg tgggcgcggc
     5761 ctcggatcgc cccgtgccgg ttgggcggaa ggtgaccctg gtggggattc gacacgcgtc
     5821 cggtcgggta gagctgcatc tagacgggcg tgtccaggcg agcgcaggtg cgggcgcggt
     5881 gatgtcctac cccgtgccga cccaggtgcg gatcgggtcg cagtacgacg gggcgagccc
     5941 ctttgccggc cggctgcacg agatggcggt ctaccggcgc ccgctgaccg gcagggaggg
     6001 gggtgcgccg acgctggcgg agttcctcgg ggaggcgaag tgatggaagc aagagcaagc
     6061 gcgagcattg ggagagtggc ccgagcggct gcctgcgccg tggggattct cctctcgggg
     6121 gtggcggcgg atggagccga gcgcttcgag gtggccgcct gggtggacca cttcgacttc
     6181 gcgggtgtgc tgcaggaaga tcgctacctc tttgattcgg agaccgccga ggggtgcgcg
     6241 cgcatcctgg accatgtgca ggaggtgggg gcgaccaccg tcctgtggcg caactgcggc
     6301 gggggcacga tgcgctacca gagccgggtg gattcgcacc ataacgatgg aatcctcgac
     6361 aagcggcggg tgcttggcaa tcgcccggtg tgggactggg tgcgctatgg cgacgccgat
     6421 ccggacctca tgcgcaccgc gctccggctg tgcaaggagc gcggcctgcg ccccggcgtc
     6481 cactggccct ttgaggagac gcactggcag atctggacca tcgggcggtt caacctggag
     6541 aacccgcagt tgtggggccg gaccgcggat ggccggccct ggtgggggcg gtgcagccta
     6601 gcccacgagg aggtgattcg gcataagctc gcgctcgtcg atgagctggt agagcggggg
     6661 atggaagttc tcttcatcga cttcttccgc accggcgggt ggagcccggc ctatgagtat
     6721 gtcgagccgg tggtgaaggc ctaccgcgag caatacggca ccgagccgcc ggtggaccat
     6781 cgcgacccgc gctggaaacg gcatgtctct cgctacgtta ccgagttcct gcgccaggtg
     6841 cgcgggcgct tgaatgcctc gggccggaag atcgagctgg ccgtcggcat tccgggcatc
     6901 gccccgctgg ctacctccga cacccttgcc tttggcgcgg actggcgtgc ctgggtagag
     6961 gaaggcctga tcgatactct ggtcatcaac actctgcact gggatgcgaa agacccgatg
     7021 gggagcaccc gggcgttggg tcgggaagtg atgcaggcgg tcaacgggcg ctgccgcgtg
     7081 ctgtggcccg tgcgggcgta cgactactcc ggctacggca tcccctccta ccggaaggcg
     7141 cttccgcaga tgacggatgc gcagatcgcg gcgcagctca ccgagatggc gtgggaagag
     7201 ggcgcggcgg ggatcagcct ggagtgcgtg gactacaaca actacgcgcc ggaaacgcgc
     7261 aaggcaatgc gcgcgttgct gcgcgataac cgttttgacg tcatcaatac gcatagctcg
     7321 acagattcat ggctggctgc gctagcttgc aagatgctgg ctgacgcccc gcccatcgtt
     7381 cgtacccgtc atttgtccac ggcggttaac cgctcatggt cgacccgctg gttgtacgaa
     7441 aaaggctgcg ctcatgtcgt cgtcaccggc gaggccctga aagcgcagtt gattcgcgat
     7501 aacggcttcc ctgctgcaca actgacctct gtgccaaccg gcatcgacct cgaacgtttc
     7561 catccgcagc cacgagcctc ggcgcgggcg gcgcttgggt tgagtgcaga ggcgacttac
     7621 atcggcattc tggccaccct gcgtgactgg aaagggcaca ccgtgctctt cgacgcgctg
     7681 actcaactgc tgccgagtcg cccgaacctg cgtgtgctga tcattggcga cggtccttac
     7741 cgtgaccggc ttgatacccg tgccgaagtg ctgggcataa ccgacaggct ggattttgtt
     7801 ggtcaccgca cggatgccga gcgctggcta ccggtcatcg atgtgtttac cttgccctcc
     7861 tggggcgacg aaggcgtgtc gcaggccttg atgcaggc
//
LOCUS       DAOSCV010000063         7897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_5949_length_7897_cov_2.959194, whole genome shotgun sequence.
ACCESSION   DAOSCV010000063 DAOSCV010000000
VERSION     DAOSCV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5949_length_7897_cov_2.959194"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..970)
                     /locus_tag="PLY56_02705"
     CDS             complement(212..970)
                     /locus_tag="PLY56_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOJ20420.1"
                     /translation="MDVPELVAATAKLKEMIGLRSEPLAFFYSDREPDGWRPPEGKWS
                     CMVGALARARHGGTVYFDKEHHGCGGCGVYLGFCEPAENLVYFVSCGIPGRLEGEHYK
                     KSPELVAAALRQNDVRPAPAKYAIFKQVAALEEGERPEVIICFANGDELAGLVFLAGY
                     AREEDAAIVPFSSGCGSIVAHPLREGRGTLPRAVLGMFDPSARPCVRAEELTFAAPVA
                     LWEEMLQNASESFLKTPTWAKLRARITGEATSES"
     gene            1107..2258
                     /locus_tag="PLY56_02710"
     CDS             1107..2258
                     /locus_tag="PLY56_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20421.1"
                     /translation="MAITLEGYEGPRPIRPEEHEAVRELSRSIFFPDRQPSQEGPRTW
                     PMALHPDVRANSLAIFYGGLPVSTTCSLERDVSLCGARLRLGYIGGVCTHPDHRKRGL
                     AGALFAASFQRFREHGVDFVYISGTRPLYYAVGANHIGSFSLFTVPPSALAGGPAVAL
                     RRATLEDAPLLCRLNERDGVHMARTSCDYELLLQHGYCAGRPCQFLIIEVEGVPAGYL
                     FGWSLEREGERQLHVLELRGERLAIRSALGLLAEEGSHVRVEVPRGDLLEALLRARGL
                     VSEPGRTPGTLKLLDFCSVMAKLRPYFASHLGAELAASLRFLEAGNRYLMLADRGAFT
                     IDGETNLLWTLLGAPAGETASGVTATGSLSDLGERCLPLPLPSVCLNLI"
     gene            2633..2708
                     /locus_tag="PLY56_02715"
     tRNA            2633..2708
                     /locus_tag="PLY56_02715"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2666..2668,aa:Lys,seq:ctt)
     gene            2904..7403
                     /locus_tag="PLY56_02720"
     CDS             2904..7403
                     /locus_tag="PLY56_02720"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ20422.1"
                     /translation="MEFARLSIRIAFALLAMLALAPAACAGQENDRPLSYVKNGGYEQ
                     GLAGWNAPSGKARVVASPRGGQCLEVDAGATATQDVYIGGNIRTYTVAVDMKASGIVP
                     QGKVGYAYVALYQLDSRGQYLVYRDFGRTSTDQEWTRHTYTFEVHPDARVLSLRCGIF
                     QAKGTVWVDNWTLVAGNTPQLLTEVREPQPFTGSTKGIVGIFNAPGFPAKGAGSRPEV
                     IASILREAGMETHFLDIAALSDPSHLRPWRYDIVVLPYGESFPAEARDSLTYYLHDGG
                     DFISMGGYTFLNPLLADGKGYAREEEVLDKRLAEAIRPVKSLLFDGGFEKPETRAVGI
                     GGSDLEARWRRTSETCQIVDEHPAEGRYCARVTSHSPKGEVQWHLRLPGEEGRRYRIS
                     AQIRAQDVTGEGFAYLAIYRYGHDGNLIKYNDFAHVRGTAPWKRYEFDFTPEPGTATL
                     FIKCGLFRAQGTIWVDDVRLGKADGTDPRPMNTATGHPGDGLITDVRQIGVFDADYRL
                     KRVQRVETAPGQTIFPAGIRLGTVRGWAASATRGYDHSRWIPLLMSYDRYGRPRGSAG
                     SLLINYSGFYAGSSWGYFGVEDRDLFDGKDPLLRKGLQRLARFMVRAAYLRNLESDFA
                     SYAPGSPVRLKVTFENRGPARQDGSVLFRVLNGGKELMRKRVPVAAGSLDHAVAETSF
                     TLPKAVAGLCHFTATLSLGGETVDSTESGFFAISPQVARRGPTLRFRDNYFRYGDRAL
                     FLFGCDNYSNVYRSTTEGPLWWAREHAACRDYGFELYENLQYNNPGHVMTEVDWRRFD
                     GMTQSTQEHGLVFMPGLLIGHNVAVSDERIREESRQCSEYAARYKEIPGLLWYINGDY
                     ALRHEDMETLRAKWNAFLKQRYGTDEKLKAAWAPEVPPAPLGALPFPPENPGRWDSVS
                     QIDLYRFHVHLMTEWNRAHVAAIRAHDAEHPITSEYYSYPRRGIDLRLTIAGQDVSNI
                     GYFDEPVRDLDYLPLRIRLNDVRVRGKSVSLGEYGVKTHPAWSLENGARGYHIVRTEE
                     EAKQLFMAVAHYTFGMGGSKVQNWCLRDSDQRVFPWGVFYPGPLIPKDIAYTHRNLSL
                     LMRAFEPRYEPPALTVLLNDNMRLGNQENLGIDSAYRCFAALLGLHADFNVASDWFAD
                     SIPASTRVIIYPSAICPGDESYTALLRWVRNGGTLLMTGAPIYDAARKPGQSARLREL
                     AGVEWVSTRYTPDVRGAQAMATLRTEIPGLAGQQAAPMVEVRPAGAKVLATDAAGKPL
                     LTKHTLGQGTVYYFADPIELAESDEGTAMHPLYRWFLEEAKIERLLVTPDNPQLHLFA
                     QRTRTGHAHVLFNYRKGDAWQEATLATKAGSITLRAKDRYPAIAGVGDDGAVTVLGAH
                     QLARVGSETLLAGEGMATAISLDELDLRKSEAVMLLPFTKGEVVLSRSQPWRRPVVLL
                     GDIQDGQFRPLERLNPAPGTRVTLPIDDDRATLVGLICEQGQEARWIKTIGQWVSDPG
                     SVPGR"
BASE COUNT         1329 a         2629 c         2611 g         1328 t
ORIGIN      
        1 ccgggaagca gcgcggagct tcgagctccg cgcgtgcggg cggccctctc cttatccaga
       61 atcccagccg gccagctggg gcccggacca ctgatgcgat ccggggggcc caacgccgag
      121 cgaggcggcg gtcatcgggc ccccggcact cgtattccat tgccagtcag tttcatgtgg
      181 cgtcgcgggc ggcgaggcct gcccggagcg cttagctctc cgaggtggct tcgcccgtaa
      241 tccgcgcgcg cagcttggcc caggtcggcg tcttcaggaa gctctcggag gcgttctgga
      301 gcatctcctc ccagagcgcc acaggcgcgg cgaaggtaag ctcctcggcg cgcacgcacg
      361 gccgggccga cggatcaaac atcccgagca cggcgcgtgg aagcgtcccc cgaccctcgc
      421 gcaacgggtg ggccacgatg ctcccgcatc ccgaggagaa ggggacgatg gcggcgtctt
      481 cttcgcgcgc gtaaccggcg aggaagacca gcccggccag ctcgtcgcca ttcgcgaagc
      541 agatgatcac ttccgggcgc tcgccctctt cgagcgcggc gacctgcttg aagatggcgt
      601 acttcgccgg tgccgggcgc acgtcgttct ggcgcaacgc ggccgccacc aactcgggcg
      661 acttcttgta gtgctcgccc tccagcctcc cagggatgcc gcaggagaca aagtaaacca
      721 gattctccgc cggctcgcag aagccgaggt agactccgca ccctccgcac ccgtggtgtt
      781 ctttgtcgaa gtagacggtg ccgccgtgcc gggcgcgtgc cagcgccccg accatgcagg
      841 accacttccc ctccgggggg cgccatccgt ccggctcgcg atccgagtag aagaacgcca
      901 gcggctccga gcgcaagccg atcatctcct tcaacttggc ggtggctgcc accagttccg
      961 gaacgtccat cctggcatct cccttcatct gattgacggg tatgttcgcc gccagcccgg
     1021 aggcccctct cttctccggg aaggttcttc acccatctcc gcgaaatagg gggcatggcc
     1081 agcatggccc agtggaggag agtaggatgg ctatcactct ggagggatac gaaggcccgc
     1141 gtccaattcg cccggaggag cacgaggcgg tgcgggaact ctcgcgctct atcttcttcc
     1201 cggacaggca gccttctcag gaaggccctc ggacgtggcc gatggcgctt cacccggatg
     1261 tccgggcaaa ctccctggct atcttctacg ggggcttgcc cgtttccacg acctgtagct
     1321 tggagcgcga cgtatcgctt tgcggcgccc ggctccgcct gggctatatc ggcggtgtct
     1381 gcacccatcc cgaccaccgg aaacggggtc tcgcgggcgc cctcttcgcc gcctcgttcc
     1441 agcggttccg cgagcacggc gtcgatttcg tgtacatctc gggcacgcgc cccctctatt
     1501 acgccgtcgg cgccaaccac atcggcagct tctcgctctt taccgtgcct ccgagcgcgc
     1561 ttgctggcgg tccggcggtg gcgctgcgcc gggccaccct ggaggacgcg ccgctcttgt
     1621 gccggctgaa cgagcgcgac ggcgtccata tggcgcgcac atcctgcgac tacgagctcc
     1681 tcctccaaca cggctattgc gccggccggc cttgccagtt cctcatcatc gaggtagagg
     1741 gcgtgcccgc cggctacctc ttcggctgga gcctggagag ggaaggggag cggcagctgc
     1801 acgttcttga gctccgcggc gagcgcctgg cgattcgcag cgcgctgggg cttctggcgg
     1861 aggagggcag ccacgtgcgt gtggaggtac cgcggggcga cctcctggag gcgctcctgc
     1921 gcgcgcgggg tctggtgagc gagccggggc gaacgccggg caccctcaag ctgctcgatt
     1981 tctgctccgt catggcgaag ctccgcccct atttcgcgag ccacctgggg gccgagttgg
     2041 cggcttcgct ccgcttcctg gaggcgggga accgctatct gatgctggct gataggggcg
     2101 cgttcacgat cgatggcgag acaaacctcc tctggaccct ccttggcgcg ccagccggcg
     2161 agacggcttc aggggtgacg gcaacgggca gcctctccga cctgggcgag cgttgcctgc
     2221 cgcttccgct tccctcggtg tgcctcaacc tgatctgacg gaatacaccc gcgaagagcg
     2281 cacgacgaga ctctgccacc gcgttatagc ggggactgct cgccagcgtc gataccacgg
     2341 ataaggggac agacacggat gggccgggcg cgcaacccag gttgcgcgcc cgagcagagg
     2401 gctggctatg gccacccgtg tggggctctc ggttcgacaa cccagaccag gatctcctgg
     2461 tccggggcct cacggccagc cctctctcag gagcccaacg tgggtccggc attcgagccg
     2521 ggagttatca cacccagaag cggttaattt ctcttcgaga atccccgttt ttctcagaaa
     2581 atgggttgac gaaatgcggg cgacctgcta taatgacacc gttgctgctg atgcgcccat
     2641 agctcagctg gcagagcagc ggactcttaa tccgtttgtc gaaggttcga atccttctgg
     2701 gcgcaccata aatatctagt aggacgaaag gctggagcgc attccgcgtt gccagccttt
     2761 tctttttggc cggctcgcgc ttctctgggt ggacctcgag tggaactggc gcgtgaccgg
     2821 caggaacccc catcaggcga tggcgaactc ctctgcaccg ctgggcgcac acgcgctcgc
     2881 tcgtggagga acggagaagt atcatggagt tcgcccgtct ctcgatccgg atcgccttcg
     2941 ccctactcgc gatgctcgcc ctggcgccgg cggcgtgcgc ggggcaggag aacgataggc
     3001 cgctctcgta cgtcaagaac ggcggctacg agcagggtct ggccggttgg aacgccccct
     3061 cgggaaaggc gcgcgtggtg gcgtcgcctc ggggtgggca atgcctcgag gtggatgcgg
     3121 gtgctaccgc gacccaagac gtctacatcg gcgggaacat tcggacctac accgtcgccg
     3181 tcgatatgaa ggcgtcgggg atcgtgccgc aggggaaggt cggttacgcc tacgtcgccc
     3241 tctaccagct ggatagccgc gggcagtatc tggtctaccg cgatttcggg cgcacgagca
     3301 ccgatcagga gtggacccgg cacacctaca ctttcgaggt gcacccggac gcgcgggtgc
     3361 tctcgttgcg ttgcggaatc ttccaggcca aaggcacagt gtgggtggat aactggacgc
     3421 tggtggccgg aaacacgccg cagcttctca cggaggtgcg cgagccccag ccgttcaccg
     3481 gctcgacgaa gggaattgtt gggatcttca acgcgccagg cttcccggcg aaaggcgcgg
     3541 gatcgcgccc ggaggtgatt gcctctatcc tccgcgaagc cgggatggaa acgcatttcc
     3601 tcgatatcgc cgctctcagt gatccctcgc acctgcgccc gtggcgctat gacattgttg
     3661 tcctccctta cggcgagagc ttccccgccg aggcgcgcga tagcctgacc tactacctcc
     3721 atgatggcgg tgacttcatc tcgatgggcg gctacacctt cctcaacccg ctgctggccg
     3781 atggaaaggg ctatgcgcgt gaggaagagg tgctcgacaa gcgcctggcc gaggcgatcc
     3841 gcccggtgaa gtcgctcctc ttcgacggcg gcttcgagaa gcctgagacg cgagccgtag
     3901 gcatcggcgg gagcgatctc gaggcgcgct ggcggcgcac cagcgagaca tgccagatcg
     3961 tggatgaaca cccagccgag gggcgctact gcgcgcgagt tacgagccac agccccaagg
     4021 gagaggtgca gtggcacctc cgcctccctg gagaagaagg ccgccgctac cgaatcagcg
     4081 cgcaaatccg cgcgcaggac gtgaccggag agggctttgc ctatctcgcg atctatcgct
     4141 atggccacga cgggaacctc atcaagtaca acgactttgc ccacgtgcgc ggcactgccc
     4201 cctggaagcg gtatgagttc gacttcaccc ccgagcccgg aacggcgacg ctcttcatca
     4261 agtgcggcct cttccgtgcc cagggcacga tctgggtgga tgatgtccgt ctggggaaag
     4321 cggatggcac cgatccgcgc ccgatgaaca ccgccaccgg ccacccgggg gatgggctga
     4381 tcaccgatgt ccggcagatc ggcgtcttcg atgccgacta tcggctgaag cgcgtgcagc
     4441 gcgtggaaac ggctcccggg cagacgatct tccccgcggg gattcgcctc gggaccgtgc
     4501 gcggctgggc ggcgagcgcc acacgcggct acgatcattc gcgctggatc ccgctcttga
     4561 tgagctatga ccgctacggc cgcccgcgcg gcagcgccgg ctcgctgctc atcaactact
     4621 ccgggttcta cgccggcagc tcctggggct acttcggcgt ggaagaccgt gacctcttcg
     4681 atggcaagga cccgctcttg cgcaaagggc tgcagcgcct ggcgcgcttc atggtgcgcg
     4741 cggcctacct gcgcaacctg gagagcgact tcgcatccta cgcgcccgga agccccgtgc
     4801 gcctgaaggt gacgttcgag aaccgcgggc ccgcgcgcca ggatggatcg gtcctcttcc
     4861 gcgtgctgaa cggcggcaag gagctgatgc gcaagcgcgt gccggtcgca gccggctcgc
     4921 tcgatcacgc ggtggcggag acgagcttca cgctccccaa ggcggtggcc gggctgtgcc
     4981 acttcaccgc cacgctctcg cttggcggcg agacggtgga ctcgacggag agcggcttct
     5041 tcgccatctc gccgcaggtg gcgcgccgcg ggcccacgct ccgcttccgc gacaactact
     5101 tccgctatgg cgaccgggcg ctcttcctct tcggttgcga caactacagc aacgtctacc
     5161 gctctaccac cgaagggccg ctctggtggg cgcgcgagca tgccgcttgc cgcgactacg
     5221 gcttcgagct ctacgaaaac ctgcagtaca acaatccggg ccatgtgatg acagaggtcg
     5281 attggcggcg gttcgacggg atgacgcagt ccacccagga gcatggcctg gtcttcatgc
     5341 ccggtctcct catcgggcac aacgtcgccg tctccgacga gcggatccgc gaggaaagcc
     5401 ggcagtgctc cgagtatgcc gcgcgctata aggagattcc cgggcttctc tggtacatta
     5461 acggcgacta tgcgctgcgc catgaggata tggagacgct gcgcgcgaag tggaacgcct
     5521 tcctgaaaca gcgctacggc acagatgaga agctgaaggc cgcctgggca cccgaggtgc
     5581 cgcccgcgcc gctgggtgcg cttcccttcc cgcccgagaa ccccgggcgc tgggattctg
     5641 tctcgcagat cgacctctac cgcttccacg tccacctgat gacggagtgg aaccgcgcgc
     5701 acgtcgccgc gatccgggcc catgatgcgg agcacccgat caccagcgag tactacagct
     5761 atccgcgccg tggcatcgac ctgcgcttga ccatcgccgg ccaggacgtc tcgaacatcg
     5821 gttatttcga tgagccggtg cgcgatctcg attacctgcc gctgcgaatc cgcttgaacg
     5881 acgtgcgcgt gcgaggcaag tcggtgagcc tgggcgagta cggcgtgaag acgcaccccg
     5941 cgtggagcct ggagaatggt gcccgggggt atcacatcgt gcgtaccgag gaagaggcga
     6001 agcaactgtt catggccgtg gcgcactata ccttcggcat gggcggctcc aaggtgcaga
     6061 actggtgcct gcgcgacagc gaccaacgcg tcttcccctg gggtgtcttc taccccggcc
     6121 cgctcatccc gaaggatatc gcctacacgc accgcaacct gagcctgctg atgcgcgcct
     6181 tcgagccgcg ttacgagccg ccggcgctca ccgtcctcct caacgacaac atgcgcctag
     6241 gcaatcagga gaacctcggg atcgattctg cctaccggtg cttcgccgcg ctcctcggcc
     6301 tgcacgccga tttcaacgtg gcgagcgatt ggttcgccga ttcgattccg gcgtccacgc
     6361 gcgtgatcat ctacccgtcg gcgatctgcc cgggagacga gagctatacc gcgttgttgc
     6421 gctgggttcg caacgggggc acgctgctca tgacgggcgc gccgatctac gacgccgccc
     6481 gcaagccggg gcaaagcgcg cgcctgcgtg agctggctgg cgtggagtgg gtgagcacgc
     6541 gctacacccc ggatgtgagg ggcgcgcaag cgatggcaac actccgaacg gagatcccgg
     6601 gcctggccgg gcagcaagcc gccccgatgg tggaggtgcg ccccgccgga gccaaggtgc
     6661 tggcgaccga cgccgctggg aagcctctgc tcacgaagca cacgctgggg cagggcactg
     6721 tctactactt cgcggatccc atcgagctgg cggagagcga cgagggcacc gcgatgcacc
     6781 cgctttaccg gtggttcctc gaggaagcga agatcgagcg cctgctggtc acgccggaca
     6841 acccgcagct ccatctcttc gcgcagcgaa cccggaccgg gcacgcgcac gtgctcttca
     6901 actaccgcaa gggcgatgcc tggcaggagg ccaccctggc gaccaaggcc gggtcgatca
     6961 ccctgcgcgc gaaggatcgc tatccggcga ttgccggcgt gggcgatgac ggcgccgtga
     7021 ccgtgcttgg cgctcaccag ctggcgcgcg tgggctcgga gacgctcctg gcgggcgagg
     7081 ggatggcgac cgccatctcg ctggacgagc tggacctgcg caagagcgag gctgtgatgc
     7141 ttcttccctt cacgaaggga gaagtggtct tgtcgcgctc gcagccctgg cgccgacccg
     7201 tggtgctgct gggagatatt caggatgggc agttccgccc tctggagagg ctgaacccag
     7261 cccccggcac gcgggtcacc ctgccaatcg acgacgaccg ggcgacgctg gtgggcctca
     7321 tctgcgagca gggccaggaa gcgcgttgga tcaagacgat cgggcaatgg gtctccgatc
     7381 ccgggagcgt gccgggccgg taatggctac ggctaccagg tagggcaggc cgagtgcctg
     7441 ccttacctgg cgcgcgctcg cgagatggca ccaccggcgc ccggccagcg ttcgcctgca
     7501 ggctgccaac aaaaacgtac gtacaccagg caggggagaa caggcccgcc gttgaactac
     7561 ggcagacgcc tagagaagcg ccgggcgtac cccgcgcgcc tggcgggaac cgggagagtg
     7621 tcacatgcta cggcgatgca ggatgacctc ggtcggatgc tggcgctggg gtgccatgtt
     7681 gggtgccctg gcgttgctca gcgctgttgg aggagaggcc atggctgaag agcgcgcacg
     7741 caaggtgacg aatctgcagg agttgcggca ggagcgaagc cgcctggcgc accggacgcg
     7801 ccggatcatc ttcaacaacg acggcaacga acccgtctac tacctcaaag aggccaccgc
     7861 gaaggaactc ctggattgcc ggaccacggc actcaag
//
LOCUS       DAOSCV010000064         7796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6038_length_7796_cov_3.541015, whole genome shotgun sequence.
ACCESSION   DAOSCV010000064 DAOSCV010000000
VERSION     DAOSCV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6038_length_7796_cov_3.541015"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLY56_02725"
     CDS             <1..320
                     /locus_tag="PLY56_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20423.1"
                     /translation="DSRFQGQTINIEGYADAAPPILSDFFVDFNLVARRSLTVSGHQA
                     VILEHQSSWAAPDGTVVPFHRYTLYTENRGNILIFTFTTPTAVFETYRPVFDQIISTI
                     ALF"
     gene            complement(399..1166)
                     /locus_tag="PLY56_02730"
     CDS             complement(399..1166)
                     /locus_tag="PLY56_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20424.1"
                     /translation="MKLKLSGRSRRGRGGLWMGAGILVVALLATAAETTANNHPIQSM
                     FAPGLKDLSLTTVRKEIHIEELNKISRDYANAYRIGRSKVMLKEPDKFRMDARVGFVK
                     FEYVMNGPRKVIRTPLTKQFDDIGTDPGKRLGPLEVGLVTPGALIGYDVEHVGCSTEE
                     GRRVCEYKLYYAHNHERYELVWIDAERKCVKKRRLYTLMYGKYKMELTYSAPKQVGNL
                     WIPTVIEVRNAEGKLAGITHQQEIKLNPGLDDSLFKL"
     gene            1577..2278
                     /locus_tag="PLY56_02735"
     CDS             1577..2278
                     /locus_tag="PLY56_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20425.1"
                     /translation="MEQKEQETPVIPVAQAQEPTGDVTQEPGAEGADLASTARSPEVE
                     VPTTGEGVPVAEGEVVEQSGTGATEAAAAAGTEVANPSAPEVRPYRPTDDYIVGERIY
                     HPVWDATGVVTERDPREQVFHMAVGGVDEPGRCHLIRVQFEKEVPASGGAKREVLLIA
                     DWHGRSLETGGTTAPRPSAVETLMGLGETSEEPISSARTTLAGLPEIPELESDLEQED
                     LEDSEGEEDIQPIGI"
     gene            complement(2489..3412)
                     /locus_tag="PLY56_02740"
     CDS             complement(2489..3412)
                     /locus_tag="PLY56_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ20426.1"
                     /translation="MGTRSHTAVLLLALAFSALAPSLARADDDRKDHVYVWGEVKNPG
                     AFPWKKGLTVPEVIRLAGGFTEAAHRNRATLTRGKREITLTADDLTPASERDAIKLER
                     GDVLRIERGALRVEGEVRSPGDYGLEHGTVRQALIAAGGPTPAADLDSVYIARGGKVI
                     RVDARALLTGGPAAENPTLEPGDVLHVPRSHSRVTIAGEVNRPGAYDLEPGKLERLED
                     LIAIAGGPTAKAQVARIELRRATRAGERPQVETINLEDPTQDELQRNPVLQSGDYVVV
                     PAQRRGRSMGINEVYQIGVLILTLVSILMGR"
     gene            3714..3790
                     /locus_tag="PLY56_02745"
     tRNA            3714..3790
                     /locus_tag="PLY56_02745"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3748..3750,aa:Met,seq:cat)
     gene            3912..6020
                     /locus_tag="PLY56_02750"
     CDS             3912..6020
                     /locus_tag="PLY56_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20427.1"
                     /translation="MKTTLCILASVLLLFAVAAEVKAQADAPDAFVQQRKQLAHRKRR
                     IIFNNDGNEVIYFPKNLPVTVENVLARRTTPLLGTHVDTIFYCPISSGFGYFTHNTKV
                     GSVLEHTWGDLGINITRPLIDQGTDALKMIVDFGRANGIEVFFSMRMNDTHDGAHTPE
                     KPYPLFPPLKAKRPDLLMGTIQNRPKHGQWSAMDYTHPEVRDLAFRFFEEVCQNYDVD
                     GIELDFWRHLVYFKSVAWGGTASDAEREMMTDLLRRIRRMADAEGRKRGRPILIAVRV
                     PDSVEYSRMVGLDIERWLAEDLVDLMVATCYFQLNPWEYIVALGHKYGVPVYPSLSET
                     RVRSEGRYRRGSQESYRARAARVWQSGADGIYLFNFFNVTAPMLREIGDPKLLAKLDK
                     TYFVNVRDYSPELYVTGGSRHQNLTVLNPSRPLSMQDGQPVTVPLYLGERVPPAPDAV
                     PQVKLCLLIPTARSPRVRLNGTALPEGELVDGWWEYRVPITAIKEGANEVEVAMASPQ
                     EVGAGEPGWDVVWTADAKPTTPWATDRARRNTALELKDGALLIADRGSEPGDYMHCVY
                     AWNARPDRESVAEFEAKVISGISYVIFSNGVAMERLRLTPDGITLTHTGLKHAVDTTD
                     RFHTYRLAIQGNDLKVYVDGKLALDASGRFTTAAPGGHNSFQFGAANSPEQGEALWRS
                     VRLRTGATSLFDVVMTFDYP"
     gene            6206..>7796
                     /locus_tag="PLY56_02755"
     CDS             6206..>7796
                     /locus_tag="PLY56_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ20428.1"
                     /translation="MVGSRSRRLGELLRERCHLSEEQIQEAVELHRKSGQRIGQALVS
                     LRYVSEKDLAWALGEQAGLPVVNLGEYPLDPDLLGLLPADLALRSQLLPLSRENGTLT
                     VACADPFNLQALDDIRLLVGCRLRPVVAPADEIKRVLEHSYMERLIQDSRDRDAEAAS
                     EEMDIADLEKMAGEAVVIRLVNVIFHQAVRDGASDIHIEPFEREVKVRYRIDGVLREI
                     SSPPRRLHPAIVSRIKILGDMNIAERRLPQDGRIRLRVAERQIDVRVSTVPTLYGESV
                     VMRILDKETALRGLPELGMDPETLARFRRLIRRPYGIILVTGPTGSGKTTSLYAALQE
                     IYSPEKKLLTIEDPVEYQLPGVNQIQVRPSIGLTFASGLRHIVRQDPDVIMVGEIRDG
                     ETAEIAIHAALTGHLVLSTLHTNDAAGAVARMLDMGAEPYLVASSLIGSVAQRLVRLN
                     CPVCKRPDEPDPDAIREVGLTAEDRARATFTRGAKCEECGFTGYRGRAGVFELLTVDD
                     EVRRMITQRTSAVEVKRYALAN"
BASE COUNT         1408 a         2475 c         2528 g         1385 t
ORIGIN      
        1 tggactcgcg cttccaggga cagacgatca atatcgaggg ttacgcggac gcggcgccgc
       61 cgatcctcag cgatttcttc gtcgacttca acctggtagc ccggcgctcg ctgacggtca
      121 gcgggcacca ggcggtgatc ctggagcacc agagcagctg ggcggcgccg gacggcaccg
      181 tggtgccgtt ccatcgctac acgctctaca cggagaacag gggcaatatc ctgatcttca
      241 ccttcaccac gccgactgcg gtcttcgaga cgtaccggcc ggtgttcgat cagatcatca
      301 gcaccatcgc tctcttctga gggcatgagc agcctcttcg tggcggccag cttccgtagc
      361 ccgaagccgg ccgccacgat ccgatccaga ggagcatgtc atagcttgaa gagggagtca
      421 tccagcccag gattcagctt gatctcctgc tggtgcgtga tcccggcgag cttcccctcg
      481 gcgttgcgga cttcgatcac cgtgggaatc cagaggttgc cgacctgctt cggggcgctg
      541 taggtgagct ccatcttata cttgccgtac atcagcgtgt agagccgccg cttcttcacg
      601 catttgcgct cggcatcgat ccagacgagt tcgtagcgct cgtggttgtg cgcgtagtag
      661 agcttgtact cgcacacgcg ccggccttcc tccgtggaac accccacatg ctccacatcg
      721 tagccgatca gcgcgcccgg tgtgaccaga ccgacctcga gcggccccag acgcttgccc
      781 ggatccgtgc caatgtcatc aaactgcttg gtcaggggcg tgcggatcac cttcctgggg
      841 ccgttcatca catactcgaa cttgacgaag ccgacacgcg catccatgcg gaacttatcc
      901 ggctccttca gcatcacttt cgaccgcccg atgcggtagg cgttggcata gtcgcggctg
      961 atcttgttca gttcctcgat gtgaatctct ttgcgtacgg tggtaagcga gaggtctttt
     1021 agccccgggg caaacatgct ctgaatggga tgattgttgg cggttgtctc tgcagccgtc
     1081 gccaacaagg ccaccaccag gatgccagcg cccatccaca gccctccacg gcctctccgt
     1141 gatctcccgc tgagcttcaa cttcatcgac tcactcctta cacgcctggg gctcctgccc
     1201 cgggccggcg gatctgacgg gagcgagcac aacggtatgc cggttggggc ttccaggtgt
     1261 ccctattagt ttggacaaca aagacgagcg ctggttcccg attcttctta gcacgaggca
     1321 agaggcacag ggcatgctgg cggcgaagag gggggtggcc ttgcgggatg atcgccctgg
     1381 tgcatatgcc gggcatgggc agatcaaacg ccctcatttc gccggttgaa tccgcttgac
     1441 aagcgagggg cgaatccgct atagtggctt cattcatatg acagacgaga cctcgttgta
     1501 ttggcggttc cccaaaccac ccggtgccct cgatagggct gttgatgcgt ggtagggatg
     1561 cagtgaaagg acggttatgg aacagaagga acaggagacg ccagtgatcc cggttgccca
     1621 ggcacaagag ccgacgggcg atgtgaccca ggaaccgggt gcagagggag cggatcttgc
     1681 cagtacggcc cgctctcctg aggtggaggt tccaaccacc ggagaaggcg ttcccgtcgc
     1741 cgagggtgag gtggtggagc aatccggtac cggcgcgact gaggcggcag cggctgctgg
     1801 aaccgaggta gcgaacccga gtgcccccga ggtgcgcccg taccgtccta cggatgacta
     1861 catcgtaggc gagcggatct accatccggt atgggacgca accggcgtgg tgacagagcg
     1921 cgaccctcgc gaacaggtct tccacatggc cgtgggcggc gtggatgagc ccggacgatg
     1981 ccacctgatc cgggtgcagt ttgaaaaaga ggtgccggcc agcggcggag ccaagcgcga
     2041 agtgctcctc attgccgact ggcatggccg gtcactggag accggtggca ccacggcgcc
     2101 tcggccctcg gcagtggaga ccctgatggg cctcggcgag acgagcgagg agcccattag
     2161 ctcagcccgc acgaccctcg ccggccttcc cgagatccct gaactggaaa gcgacttgga
     2221 gcaggaagat ctggaagact ccgagggaga agaggatatc cagcccatcg ggatctaagt
     2281 ggaatgcgcg accgaacggg cagaaacgga acgcaggccg cctcttctat tgaggccggc
     2341 gaggggacgt tctcgttgcg catggagcgc ccgttcgctc gcgcactcgg cttgttcagc
     2401 gaaccttcct aattcgcgtg acgcgctctg ggtccgggag taggcgagag gtgcgcctct
     2461 cgagctcccg gatcccggaa ccgcgccatc agcggcccat caggatgctc accagagtca
     2521 gaatcagaac gccaatctgg tagacctcat tgatccccat acttcgaccc cggcgctggg
     2581 caggcaccac gacgtaatcg ccactctgca acaccgggtt gcgctgcagc tcatcctgcg
     2641 tgggatcttc caggttgatc gtctccacct gggggcgttc tcccgcgcgc gtcgcccggc
     2701 gcagctcaat ccgagccacc tgcgcctttg ccgttggccc cccggcgatg gcgatcaagt
     2761 cctctaagcg ttccagcttg cctggctcca ggtcgtaggc gcccggccgg ttcacctcgc
     2821 ccgcaatcgt gacccgtgag tgggatcgcg gcacgtgaag cacatccccc ggctccagcg
     2881 ttgggttctc cgctgccggc ccgcctgtga gaagcgcgcg cgcgtccacc cgaatcacct
     2941 tgcccccgcg ggcgatgtaa accgagtcca ggtcagcggc gggggtcggg ccgcccgccg
     3001 cgatcagcgc ctgtcgcacg gtgccgtgct ccaggccgta gtcgcccggg ctgcgcacct
     3061 ccccttcaac gcggagggcg ccgcgctcga tgcggaggac atctccgcgc tccagtttga
     3121 tggcatcgcg ctccgaagcc ggcgtcaggt catccgcggt cagcgtgatc tcgcgcttcc
     3181 cgcgagtgag cgtggcccgg ttgcggtgcg ccgcctccgt gaagccaccg gccagacgga
     3241 tcacctccgg gacagtcagc cccttcttcc aggggaacgc gcccgggttc ttaacctcgc
     3301 cccagacata gacgtggtct ttgcggtcgt catccgccct agcaagagag ggcgcgagcg
     3361 ctgagaaggc gagagcgaga agcagaacgg cagtatggct gcgggttccc atgcgaatgc
     3421 tcctccttat tcgagtgacc gcacgcggca gccaggggcc ggcggtcggc tgcattatag
     3481 cacccgccgg agaccgagcg caacttgggc cgtgccggcg ccgatatcac ggaagaaagg
     3541 gactgacccg gatggggaga tgcgggcgct cccgggcagc gagccgtttg ggagcgaggt
     3601 ggcttcccgg cgtgggttct cggcacgccc acgggcttac ggagcgtgtg gaaagattca
     3661 tgcgaatagg gggttgacaa agggaaaata acctggtatg atatgggtgt tgacgcgggg
     3721 tggagcagtc tggtagctcg tcgggctcat aacccggagg tcgtgggttc aaatcccacc
     3781 cccgctccca agtgaaaagc cctgatagcg ggaacgctat cggggctttt tttgattctg
     3841 ggccacgatt cggccactgg cgaggtgaat tcccggaagg gatccccggc tgggggccga
     3901 acatcggggg catgaagacc acactctgca tactcgcgtc cgtgttgttg ctgtttgccg
     3961 tcgccgccga ggtgaaggcg caggccgacg cgccagatgc ctttgtccag cagcgcaagc
     4021 agctggccca ccggaagcgc cgcatcatct tcaacaacga cggcaacgag gtcatctact
     4081 tcccgaagaa cctgccggtg acagtggaga atgtcctcgc gcgccggacg acgcccctcc
     4141 tcggaaccca cgtggatacg atcttctact gtcctatcag ctccggcttt ggctacttca
     4201 cgcacaacac gaaggtgggc agcgtgctgg agcacacgtg gggcgacctg gggatcaaca
     4261 tcacccggcc gttgatcgac caggggaccg atgccctcaa gatgatcgtc gatttcgggc
     4321 gcgcgaacgg gattgaggtc ttcttctcga tgcgcatgaa tgacacgcat gacggcgcgc
     4381 acacgccgga gaagccctac ccgctcttcc cgccgctgaa ggcgaaacgc ccggatctcc
     4441 tgatgggcac catccagaac cgccccaaac atgggcagtg gtcggccatg gattacaccc
     4501 acccggaggt gcgtgacctg gccttccgct tcttcgagga agtctgccag aactacgatg
     4561 tggacggcat cgagctggac ttctggagac acctcgtata cttcaagtcc gtggcctggg
     4621 gcggcaccgc gagcgacgca gagcgggaga tgatgaccga cttgctgcgc cgcatccgcc
     4681 gcatggcgga tgccgagggg cgcaagcggg gccggcccat cctcattgct gtccgggtgc
     4741 ccgactccgt cgagtactcc cgaatggtgg gcctggatat cgagcgttgg ctcgccgagg
     4801 acctggtcga tctgatggtg gcgacctgct acttccagct caatccgtgg gagtacatcg
     4861 tcgcgttggg gcataagtac ggggttcccg tctacccgtc gctgagcgag acgcgggtcc
     4921 gatcggaggg ccgatatcga cgaggctctc aggagagcta tcgcgcgcga gccgcccggg
     4981 tatggcagag cggcgccgac gggatctacc tgttcaactt cttcaacgtc acggcgccca
     5041 tgctgcgcga gatcggcgat ccaaagctgc tggccaagct ggacaagacg tactttgtga
     5101 atgtgcgcga ctattcgccg gagctgtacg tcacgggcgg gagccgccac cagaacctga
     5161 cggtgctcaa cccgagccgg ccactctcga tgcaggatgg ccagccggtg accgtgcctc
     5221 tctatctggg cgagcgggtg ccgccggcgc cggatgcggt gccgcaggta aagctgtgcc
     5281 tcctgatccc caccgcgcgt agcccgcggg tgcggctgaa cggcacggcg ctccccgagg
     5341 gtgagctggt ggatggctgg tgggaatatc gcgtgcccat caccgcgatc aaggagggcg
     5401 ccaacgaggt ggaggtcgcg atggcttcgc cgcaggaggt cggcgctgga gagcccggct
     5461 gggacgtagt gtggaccgcg gatgcgaagc caacgacacc gtgggccacg gaccgagccc
     5521 ggcgcaacac ggcgctcgaa ctgaaggacg gtgcgttgct gatcgcggac cgggggagcg
     5581 agccgggaga ttacatgcac tgcgtgtacg cctggaacgc ccgcccggat cgagagagcg
     5641 tggcggagtt tgaggcgaag gtgatttccg ggatcagcta tgtgatcttc agcaacggcg
     5701 tggcgatgga gcggctgcga ctgacgcccg acgggatcac cctcacacac accggcctca
     5761 agcacgcggt ggacaccacc gaccgcttcc atacctaccg cctggccatc caggggaacg
     5821 acctcaaggt atatgtggac ggaaaactcg ccctggatgc cagcgggcgg ttcacgaccg
     5881 cggcgcctgg cgggcacaac tccttccagt tcggagcggc caacagcccc gagcaaggtg
     5941 aggccctgtg gcggtcggtc cgcttgcgaa ccggcgcgac cagcttgttc gacgtcgtca
     6001 tgactttcga ttacccttag ccgtgtggcg atctctccgc gcccgtcgcc ggtccccggc
     6061 ccctcggccg gggactggcg tgtaccaggc atcgaccgct gacttgggcc ttaacagttt
     6121 cgtaacattg tctcgcctgc ctgatgcaac atcgcgggga gctgtcagtc taaatagtaa
     6181 cagcatgttt tggaaaaggt gatacatggt tggttcgcgt tcccgccgcc ttggtgagct
     6241 gcttcgcgag cggtgccacc tctctgagga acagatacag gaagccgttg aactacaccg
     6301 caagagcgga cagcgcattg gacaggcgct cgtgtcgctc cgctacgtga gcgaaaagga
     6361 tctggcctgg gcgctcggcg aacaagccgg attgccggtg gtgaacctgg gcgagtaccc
     6421 tctcgacccg gacctcctgg ggctcctccc ggcggacctc gctctgcgct cccagcttct
     6481 gccactctcc cgagagaacg gcacgctgac ggttgcctgt gccgatccct tcaacctgca
     6541 ggcgctcgat gatatccgcc tcctggttgg ctgccgcctg cgtcctgtgg tcgccccggc
     6601 cgacgagatc aagcgcgtcc tggagcactc ctacatggag cgcctcatcc aggatagccg
     6661 agaccgcgac gcggaggcgg cgtccgagga gatggatatc gccgacctcg agaagatggc
     6721 cggcgaggcg gtagtcatcc ggctcgtgaa cgtgatcttt caccaggcgg tgcgtgacgg
     6781 cgccagtgat atccatatcg agccgttcga gcgcgaggtg aaggtgcgct accggattga
     6841 tggcgtgttg cgagagatct cctcgccgcc gcggcgcctg catccggcca tcgtatcgcg
     6901 catcaagatc ctgggcgaca tgaacatcgc cgagcggcgc ctgccgcagg acgggcgcat
     6961 ccgcctgcgc gtggccgagc ggcagatcga cgtgcgcgtc tccacagtgc ctacgctcta
     7021 cggcgagtcg gttgtcatgc gcatcctcga taaggagacg gcgcttcgcg gcctccccga
     7081 actgggcatg gatccggaga cgctggcgcg cttccgccgg ctcatccgcc gcccctatgg
     7141 gattatcctg gtgaccggac caaccggaag cggcaagacg actagcctct acgccgccct
     7201 ccaggagatt tactccccgg agaagaaact attaaccatc gaggatccag tggagtatca
     7261 gctcccgggc gtcaaccaga tccaggtgcg ccccagcatt gggctgacct tcgcctcggg
     7321 cctgcggcat atcgttcgcc aggacccgga tgtcattatg gtgggcgaga tccgcgatgg
     7381 cgaaacggcc gagatcgcga tccacgccgc gctcacgggc cacctggtcc tcagcacgct
     7441 acacaccaac gacgccgcgg gggccgtcgc acgcatgctg gatatgggcg cggagccgta
     7501 cctcgtggcc tcatcgctca tcggaagcgt ggcccaacgc ctggtgcgct tgaattgccc
     7561 cgtctgcaag cgtccggatg agcccgaccc ggatgccatc cgagaagttg ggctcaccgc
     7621 ggaggatcgg gcgcgcgcga cctttacgcg gggcgccaag tgcgaggagt gcggcttcac
     7681 cggctaccgc gggcgcgccg gtgtcttcga gctgctaaca gtcgatgatg aggtgcgccg
     7741 catgatcacg cagcgtactt cggcagtgga ggtcaagcgg tatgccctgg cgaacc
//
LOCUS       DAOSCV010000065         7749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6085_length_7749_cov_3.172862, whole genome shotgun sequence.
ACCESSION   DAOSCV010000065 DAOSCV010000000
VERSION     DAOSCV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_7749_cov_3.172862"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..588)
                     /locus_tag="PLY56_02760"
     CDS             complement(163..588)
                     /locus_tag="PLY56_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20429.1"
                     /translation="MTRVWITRGSRPQAGYTLIEVVMAILVICIALLGVFQMVGGAIG
                     TTADSRNRALAMRFAERQLEAARGEVVSSLSTSTGNTHPQLSQWLGASARWDLTVMDV
                     GPRLKGITVTVRWRDGTLDQEISLSTLAHTAGIASIGAR"
     gene            complement(930..1952)
                     /locus_tag="PLY56_02765"
     CDS             complement(930..1952)
                     /locus_tag="PLY56_02765"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20430.1"
                     /translation="MQTYNQPPSILEQPGILTLTEEPVLHPAIFGKPYVLNAGVAEYE
                     GSIILLSRAQAASEPSSIWMAVLDPDGTTVREVIREPVLGPGARCEVSPHGVEDVRIT
                     PMSDGSYIITANSLWPDEIHGGWWMPQIAIIRTWDFQEFELIGFPFPGLATKNSTLFP
                     DTDSNVRYLLHRVQPDIWLARTTDTTLRHWPDPGRPIMRPRQHLVEAGLLKWIGAGSQ
                     PMETSEGWLMVYHSGMWISEDHGQKLYLLWLALLDRDDPSKILAQSRDPIYWPLLGEN
                     PREEDWYSGVSFTCGALIREAELWVYMTLNDSRIHLGKMPMETVWRLLDRTTPSDGAA
                     AKPHEG"
     gene            complement(2503..3561)
                     /gene="recA"
                     /locus_tag="PLY56_02770"
     CDS             complement(2503..3561)
                     /gene="recA"
                     /locus_tag="PLY56_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ20431.1"
                     /translation="MAVQSNQREEALNRALLQIEKQFGKGSVMRLGERPRQQVEAIPT
                     GCLPLDMALGVGGLPRGRIVEVYGPESSGKTTVALQVIAEAQRQGGIAAFIDAEHALD
                     PVYAANLGVDVDNLLICQPDYGEQALEITDVLVRSGTIDVVAVDSVAALVPKAEVDGE
                     MGDSFVGVQARLMSQALRKLSGTLSRTNTACIFINQLREKIGVMFGNPEVTPGGRALK
                     FWASVRIEVRRIETLKQGTEVVGNRVRAKVVKNKVAPPFREAEFDIIFGKGVSRAGGL
                     LDVADKMGVLTKSGTWWSYGDERIGQGRENARIFLEEHPEVAAEIEARIRASLQPVSV
                     EDLPVSTADEGEIDPALE"
     assembly_gap    4077..4086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4173..5420)
                     /locus_tag="PLY56_02775"
     CDS             complement(4173..5420)
                     /locus_tag="PLY56_02775"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HOJ20432.1"
                     /translation="MEQEGLSSSGMRKRRPVAAVLAIGVACAFLLCGYEFIRSVSTSF
                     YIDAYGAHRLPVVMGLMPVGVALTLYGYGVLLSWFGPARALLLTSGFSAALITACWAA
                     LRVGWHPAAGILYVFREAYIVVVLEQYWSLINSALTAGQAKRLNGPITGLGSLGGILG
                     GSLVHAFATRVGSEMFLLFAAGSLIPAALLSAVAYHLAGEPQPSPEEAGGRQGHLALR
                     LFARSRYLLLLAALIALTQVVSTALDLRFSGLVEEAFPVKDERTAYLGGFYGTLNAVA
                     ALLQFVVAPVALRWLPLPMIHLGIPLVHLVTSLLLLASPSLHTGAAAYLLFKGLDYSL
                     FRAGKEILYIPLTFDVRYRAKEVIDAFVYRFSKGALGMLLTGVNAAIGSIPAAVFPAV
                     AALASLIWIPVVFRLTRQPEGKR"
     gene            5672..6583
                     /locus_tag="PLY56_02780"
     CDS             5672..6583
                     /locus_tag="PLY56_02780"
                     /inference="COORDINATES: protein motif:HMM:NF027889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HOJ20433.1"
                     /translation="MSDDAYAPQATWPEETSRVLDRVVERGGTVLLLGATDTGKSMLA
                     RELLNRGLHAGKSVALVDADVGQSDIGPPTTIGLGIPETPVTHLAQVPPRRLYFVGDT
                     TPARHLLPVVLGTSLLAIAARSRGCRLIVVDTTGMVFGRLAATLKFHKIQAVRPRCIL
                     AIQARRELEPILRPFARPGGPVLVRLPVAPGVRVKSPQARAENRKRSFAAYFSSAHEQ
                     HLSYSRVSLWPPTLPKVLGDAPQRICGLLDAHGETLAAGILLSANDSGLTVLTPLADI
                     AQVATVQLGDLQVTPQGEELGRPARRG"
     gene            6845..7267
                     /locus_tag="PLY56_02785"
     CDS             6845..7267
                     /locus_tag="PLY56_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HOJ20434.1"
                     /translation="MAQKRDPAPSQNRWHRPLGDLEQAVMDVVWSHGPIGSPGVQAAL
                     APARQLALTTVVTTLDRLYKKGLLRRERVGKGYQYSAAVSRSDLEQRIVRGVLADLMA
                     EFPDALTSLLEEDAALPPDVYDRLSEWLRRQREEAEDA"
     gene            7260..>7749
                     /locus_tag="PLY56_02790"
     CDS             7260..>7749
                     /locus_tag="PLY56_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20435.1"
                     /translation="MRSLALTPAGLTLFLATAAILLAGTALAARWLPWFRRGSPDLRA
                     DRLLLAVLCPLLASGAITLFVMATLVEDLLAFHQVPHLCLDPGALSTAGRFAVFALEG
                     GTLLALVATGCQMARAIQLLRQYERAIDGQQLGLSDQAAAAVARVRERLECTAEFCEA
                     DLP"
BASE COUNT         1354 a         2646 c         2457 g         1282 t
ORIGIN      
        1 ccgcggccaa gagggcaatc gcggtcgcga tgatcacctc tgtgagggtg tagccggcgc
       61 tatggcggct gcgcgcgctg ctcatcgagt tcctccagat gccttgccgc gagagaggcc
      121 gccatcctgc tcgcgggccc ctggctcagt ggcacccacc cctcagcgcg ctccaatcga
      181 cgcgatgccc gccgtatgcg ccagcgtgct aagcgagatc tcctgatcaa gcgtgccatc
      241 tcgccatcga acggtgaccg tgatgccttt gagccgcggg ccaacatcca taactgtgag
      301 atcccacctc gcgctcgcgc ccagccattg ggagagttgc ggatgggtgt tgcctgtgct
      361 cgtgctcagg ctggagacca cttcaccccg ggctgcctcc aactgcctct cggcaaagcg
      421 cattgccagg gcacggttgc gcgaatcggc ggtcgtgccg atggcgccac cgaccatctg
      481 gaacaccccc agcagggcga tgcagatcac caggattgcc atcaccactt cgatgagcgt
      541 gtagcctgct tgcggacggc ttccccgagt gatccagacg cgcgtcatca ggatcggcct
      601 ctctcttctc tcaccggtat cgcctcaaga agggacgtgc ccggcgacga gtactcccta
      661 tctctcctga atctattgtc ggatcgcccg cggtggttct gtagggtggg gcaaggcttc
      721 ccagtcctcg cctggcaagg gttcggatgg cgacccggcc tgccgggccg cgtcaggcca
      781 ggagatgcca caaccggttg aagcccgcta tcgcgccgag acggggcgtg cagcacaagc
      841 gacatcgagc cagcaaaggc gtcttgtgcc gcccgatcgg tgttcgttcg gcatggccgg
      901 cgcggtcgtt acccggcgcg gtggccgctt cacccctcat ggggcttggc agccgcccca
      961 tccgaagggg tggttcggtc cagcaaccgc cagaccgtct ccatcggcat cttgcccagg
     1021 tggatgcggc tatcgttgag ggtcatgtag acccacagct ccgcctcgcg aatcagggcg
     1081 ccgcacgtga aggagacgcc ggaataccaa tcttcctcgc gcgggttttc gccaagcaag
     1141 ggccagtaga tcggatcgcg cgactgcgcc aggatcttcg aggggtcatc gcggtccagg
     1201 agcgccagcc agaggaggta gagcttctgg ccgtggtcct cgctgatcca catccccgag
     1261 tggtagacca tcagccaccc ctccgatgtc tccatcggct gggaaccagc cccaatccat
     1321 ttcaacagcc cggcttccac caggtgttgc cggggtcgca tgatcggccg ccctggatcc
     1381 ggccaatgtc tgagggttgt atcggtggtg cgtgctagcc agatgtcggg ctgcacccgg
     1441 tggagcaggt agcggacatt gctatcggta tccgggaaga gggtggagtt cttggtggcc
     1501 aatcccggga aggggaagcc aatcagctcg aactcttgga aatcccaggt gcgaatgatg
     1561 gcgatctgcg gcatccacca tcccccatgg atctcatccg gccacagcga gttggccgtg
     1621 atgatgtagg agccatcgct catcggggtg atgcgcacgt cctctacgcc gtggggcgac
     1681 acctcgcagc gcgcgccggg gccgaggacg ggctctcgga tcacctcgcg caccgtggtc
     1741 ccatcgggat ccagcaccgc catccagatc gaggaaggct cgctggctgc ctgggcccgc
     1801 gacaagagga tgatgctccc ctcgtattcg gcgactccgg cattgaggac atacggtttc
     1861 ccaaaaatgg ccggatgaag caccggctcc tcggtcagtg tgaggatccc cggttgctcg
     1921 agaatggatg ggggctggtt gtacgtctgc atgcgattgt ccatgggtta tgcacgcttc
     1981 acgagaccta ccagcgtccc gcctgttccc ttcgcgtcat tgttcacccc gcacctgtag
     2041 cccgcaagca tcgtgccgca ccgcgaacga ttgggagggg ccactggacg ccagttcgag
     2101 actgccggca cgcctgaccg cctcagacaa cggaccggcc taccacctga ttcgccctcc
     2161 cgttatcgac tcctcccccg ttcgtcgcca ctctccacgt gcgcgagccc acgtgccggg
     2221 aacgcgaatt acgaacggga cgatacccca actgaaggat gcggacgcag atgggcatct
     2281 gaccggcacc cacttgggga agccacacgc gggggcatag cgaggcgccc caagccggga
     2341 gggttgcgag cagggcatcc tcgtccgcag gatgagcggt atggcgggag gaaggaaggc
     2401 ggaggggcgc tcccgatggg cgttggagct caagcccacg ccacgcatga gagaggttca
     2461 ggcgccggct gggtgccggc gcctcgtgca gcagatcacg cctcactcaa gagcagggtc
     2521 aatctccccc tcatcagccg tggagaccgg cagatcttcc acgctcaccg gctgcaggga
     2581 agcgcggatg cgtgcctcga tctccgcggc gacctcgggg tgctcctcga ggaagatgcg
     2641 cgcgttctcg cggccctggc cgatccgctc gtcgccgtag ctccaccagg tgcccgactt
     2701 ggtgagcacg cccatcttat cggccacgtc gagcaggcca ccggcccggc tgacaccctt
     2761 gccgaagatg atgtcgaact ccgcctcgcg gaaggggggc gccaccttgt tcttcaccac
     2821 cttcgcccgc acgcgattgc ctacgacctc ggtgccctgc ttcagcgtct cgatgcggcg
     2881 cacctcgatc cgcacgctcg cccagaactt cagcgcgcgc ccgccggggg taacctcagg
     2941 gttgccgaac atcaccccga tcttctcgcg aagctggttg ataaagatgc aggcggtgtt
     3001 ggtgcgcgaa agcgtgccgc tcagcttgcg aagcgcctgg ctcatcagcc gggcctggac
     3061 gccaacgaac gagtcgccca tctcgccatc cacctcagcc ttgggcacca gcgccgccac
     3121 cgagtcgacc gccaccacgt cgatggtgcc gctgcgcacc aggacgtcgg tgatctccag
     3181 cgcctgctcg ccatagtccg gctggcaaat gaggaggtta tcgacatcca cccccagatt
     3241 agccgcgtag accggatcga gcgcgtgctc cgcatcgatg aacgccgcga tcccgccctg
     3301 gcgctgagcc tcggcgatca cctggagcgc gaccgtcgtc ttcccgctcg attctgggcc
     3361 atacacctcg acgatccggc cacggggaag tcctcccacg cccaatgcca tgtccagggg
     3421 caggcagccg gtcggaatgg cctccacctg ctgccgaggc cgctcgccca ggcgcatcac
     3481 cgaccccttg ccaaactgct tctcgatctg caacagcgcc cggttgaggg cctcttctcg
     3541 ctgattggac tggaccgcca ttctcttgcc tccttggtga acgcacacca cggaaacagg
     3601 gcctgcttca caaccgaaga cgccactgcc tgccgtcgtg gctactatcg atcttgcgaa
     3661 actggtgagg gtggagccgg gcagcctttc tgccgcaaac ccgggcagaa caggcttgca
     3721 tccccgctta tcgggccacg gcgtcgccgc catgtggccg cctccaccag agattataac
     3781 atggggcacg ctccgaagtc aaacaacagt tctcccgcca gccgccgggc aggaaggcgc
     3841 aggcggcgtg tcttcagcga tccccttctc gcttgcggat tcgacctgaa gattcagggc
     3901 gagcgtccga taatcctatg ggggagagcc agcggatgac agtagaacgc ccgctctgcc
     3961 atccctgcaa tccgccggca acggatcgac ctcccccgcg gcattatcgc acggctgcgg
     4021 cagcaagccg cggctcgtga catgatgttc ccaatccgca gggagctggt agcatcnnnn
     4081 nnnnnncggc tccctgcacc ctctctgggg cccctcgctc gcggcaggcg gaaagctcag
     4141 tgccctccgg tcgcgggcct ctcccctcgc gcttaccgct tcccttccgg ctggcgtgtc
     4201 agccggaaga ccaccgggat ccagatcagg gaagccagcg cggcgacggc tgggaagacc
     4261 gcggcgggaa tcgacccgat cgccgcgttc accccggtca gtagcatccc gagcgccccc
     4321 ttcgagaagc gatagacaaa ggcatcgatc acctccttgg cgcgatagcg cacgtcgaag
     4381 gtgagcggga tgtagagaat ctccttgccg gcgcggaaga gcgagtaatc gagccccttg
     4441 aagagcaggt aggcggccgc tccggtatgc agcgagggag atgccaggag gagcaggctg
     4501 gtgacgagat ggaccagcgg aatgcccagg tgaatcatgg ggagtggcaa ccagcgaagc
     4561 gccacgggag ccaccacgaa ctggagaagc gcggcgacag cgttcagagt tccatagaag
     4621 ccacccagat acgccgtgcg ctcatccttc accgggaaag cctcttccac caggccggaa
     4681 aagcggagat cgagcgccgt ggagacaacc tgagtgaggg cgatcagcgc ggccaggagg
     4741 agcaggtagc gagagcgagc gaagagacga agcgccaggt gcccctgccg tccgcccgcc
     4801 tcttccgggg acggctgtgg ctcgccggca aggtgatagg cgaccgccga gaggagcgcc
     4861 gcggggatga gagagcccgc cgcgaagagg aggaacatct cggagcccac ccgagttgcg
     4921 aaggcgtgca cgagcgagcc tccaaggatc ccgccgaggg agcccaggcc ggtgatcggc
     4981 ccgttgagcc gcttcgcctg cccggcagtg agcgcgctgt tgatcagcga ccagtactgt
     5041 tccagcacca ccacgatata ggcttcgcgg aatacgtaga ggatccccgc ggccgggtgc
     5101 cagccgacgc gcagggcagc ccagcacgcc gtgatgagcg cagcggaaaa gccggacgtg
     5161 aggagcaacg ccctcgccgg accaaaccac gaaagcagaa cgccgtagcc gtagagcgtg
     5221 agcgcaacgc ccacgggcat cagccccatc accaccggga ggcggtgggc accataggca
     5281 tcgatgtaga acgaggtgga gacgctgcgg atgaactcat agccgcagag gaggaacgcg
     5341 cacgcgacgc cgatagcgag caccgccgcg accggccggc gcttcctcat cccgctgctg
     5401 ctcaaaccct cttgctccac cgcatcttct ccccgccggc atcctgcggc cggccacaat
     5461 gttctctatt agaggccccc tcccgcgttt cctctccttc cctccgcatc ggtattcggt
     5521 ctgcgaccgc gcaggcgcgg gcagtcggag gcgcgttccg cgtgagcgaa agcgatgcgg
     5581 ctccatcgca tccgagagcg gttcgccggg cacggcgagc gaagcacgcg cggcaggagc
     5641 caccgcctcg cacatggaac cccccgtcat catgagtgac gatgcgtatg caccccaggc
     5701 gacctggcct gaagagacca gccgcgtgct ggatcgcgtg gtggagcgtg ggggcacggt
     5761 tcttcttctg ggcgccacgg atacgggcaa atcgatgctg gcgcgcgaac tcctcaaccg
     5821 ggggttgcac gcagggaagt cggtggccct cgtcgatgcc gacgttgggc aatcggatat
     5881 cggcccgcca acgaccatcg gcctgggtat ccccgagacc ccggtcacgc acctggcgca
     5941 ggttccccca agacgtctct acttcgtggg cgataccacg cctgcccgcc atcttctgcc
     6001 ggttgtcctc ggaacatcgc ttctggcaat agcagctcgc tcgcgtggat gtcgcctcat
     6061 cgtggtggat acgacaggga tggtcttcgg acgtctcgct gccacgctga agttccacaa
     6121 gatccaggcg gtgcggcccc ggtgcatcct cgccattcag gcgcgcaggg agctggagcc
     6181 aatcctccgg cccttcgctc gcccgggtgg cccggtgttg gtgcgcctcc ccgtggcacc
     6241 tggtgtgcgc gtcaaatcgc cccaggcgcg cgccgagaac cggaagcgct cctttgccgc
     6301 ctatttttcg agcgcccacg agcagcacct ttcctattca cgcgtcagtc tctggccgcc
     6361 aacccttcct aaggtgttgg gggatgcccc ccagcgcatc tgcggtctcc tcgacgccca
     6421 cggcgagacc ctggcagccg ggattctcct gagcgccaat gactccgggc tcacggtgct
     6481 cacgcctctg gcagatatcg cccaggtggc cacggtgcag ctcggcgatc tccaggtgac
     6541 tccgcaaggc gaggagcttg gccgacccgc tcgacgcggt tgagataagg aatccgcttc
     6601 cgggcacagg aactaccggc tcgaatgccg gacctcttct accgtagtcc cgcagatggc
     6661 catcctttag ccccgaagag gccacacaac tcagcccagg gtgcccacac cctcggtgaa
     6721 ctacctgagc aacaacaaat gccgccgcgc ttccttgaca ggttgttcga ccggcgatag
     6781 aatgagcatg gttctacgcc tggtcgaagc aacaggtgag gtggggagga gctggcgagc
     6841 accaatggcc cagaagagag atcccgcgcc atcccagaat cggtggcaca ggcccctcgg
     6901 cgacctggag caggcggtga tggacgtggt ctggtcccac gggccaatcg gttcgcccgg
     6961 ggtgcaggcg gcgctcgcgc ccgcgcgcca gctggcgctg acgaccgtcg tcacgacact
     7021 cgaccggctc tacaagaagg gccttttgcg ccgggagcgg gtcggcaagg gctaccagta
     7081 ctcggcggcg gtgagccgtt cggatctgga gcagcggatc gtgcgcggcg tcctggcgga
     7141 tctgatggcc gaattccccg atgccctcac cagtctcctg gaggaggacg ccgcccttcc
     7201 cccagatgtt tacgaccggc tctccgagtg gctgcgccgg cagcgggagg aggcagagga
     7261 tgcgtagcct cgcgctcacg ccggcgggcc tcacgctctt cctggcaacg gctgctattc
     7321 tcctcgccgg caccgccctg gccgcgcgtt ggctcccctg gttccggcgt ggctcgcccg
     7381 acctgcgcgc ggaccggctc ctgctcgcgg tcctctgccc gctcctcgcc tcgggcgcca
     7441 tcaccctctt cgtgatggcc accctggtgg aggacctgct cgctttccac caggtgcccc
     7501 atctctgcct cgaccccggc gcgctctcca cggctggcag gttcgccgtc ttcgcactgg
     7561 aaggcggcac gctcctcgcg ctggtggcca cgggatgcca gatggcgcgg gcgatccagc
     7621 tgctccggca atatgagcgt gcaatcgacg ggcagcaact cggactatcg gatcaggcag
     7681 ccgccgccgt ggcgcgcgtg cgcgaacgcc tggagtgcac cgcggagttc tgcgaggctg
     7741 acctgcctc
//
LOCUS       DAOSCV010000066         7621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6215_length_7621_cov_3.280333, whole genome shotgun sequence.
ACCESSION   DAOSCV010000066 DAOSCV010000000
VERSION     DAOSCV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6215_length_7621_cov_3.280333"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLY56_02795"
     CDS             complement(<1..590)
                     /locus_tag="PLY56_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ20436.1"
                     /translation="MVTLLHLADLHLDTPFKGLAETDEALATRLRDATLNALDRAVAF
                     ALEQKLSGRLDAVTFGGDIFDETAPSLRARMRFGEALKTLCREGIPCFIIAGNHDPID
                     DPSLWRRHVSLPEGCTLFDAEWGAGVIEKEGAPACTVVGRSFSQALNLAHPLAGLEAH
                     AAALEVAPLRVGLLHCDAGGTMSEMNGYAPVSVGDLA"
     gene            1030..3360
                     /locus_tag="PLY56_02800"
     CDS             1030..3360
                     /locus_tag="PLY56_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20437.1"
                     /translation="MVKDLFDAQATGPNLLSDDKWQPWEQGFERQGGVFVCDNGNDNT
                     VHRGVGQTVELNQDVPRPIIAVAWSKAEGISGGRDNNYSLYLDLLYADGTPLWGQTAP
                     FSVGTHDWERRQVVIFPSKPVKQVSFYMLLRRHTGKASFRDPVLYQLETPKGAAIFDG
                     LPVTVKGRLAEGFQVRDVAANTGFSTFEGGKAFGLTLESQRQQRDGATFIRCRIADTT
                     GKDRAITLVYTVPVGNQGWRWLADPRRSLETQPPVEYHEVSRFAAGAIGALSRYPLAA
                     IARGNTGRAIGLDMGYPAFYRVGYSAGTGELFIAYDLGLTPEKPSAELQFCLYDFDAA
                     WGFRSALDRYYRLFPELFRCRTPEQGLWMPFAKISQVEGWQDFGFKFKEGNDETAWDD
                     ANNIITFRYTEPMTWWMRMPEEMPRTMEAAVAEAKRLAGEGNRQAQALLASGYHDTNG
                     QFVARLLDTPWCNGAVWSMNSAPGVPGETTDFLNKWSPKLREQLYGPERKGDLDGEYI
                     DSSEGYVTDELNFRREHFAGMKTPLTFDIGSHRPAIFRGLIAYEYARAMAEDVHRMGK
                     LMMANSTPSRLPWLAAYMDVLGTETNWNPRGNWQPMSDADLLYRRAMCATKPYCFLMN
                     TDFNLFSHELVEKYMKRSLAYGMFPGFFSADASTNHYFRNPALYNRDRPLFKKYVPLC
                     KRVAEAGWRPITGAVTSDPQVYVERFGDRYLTLFNDSSEEKRFTVTLQGKAPAASRDL
                     VTGQEVRWRAGGTLHGQPAARAEFTLKAEDVAVIEF"
     gene            3467..4435
                     /locus_tag="PLY56_02805"
     CDS             3467..4435
                     /locus_tag="PLY56_02805"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOJ20438.1"
                     /translation="MSHYRCAEGRLATGEREFRLVDEAAGVDITVIPDQGAVWSAFGA
                     RDESGNRMELFHPAEHFTYPGGSPVLFPVTGRSACDGELGFYRHRGTRYPMPIHGFAR
                     DLPWDVEDVAADEAGARITCSLQDTEETRRVYPYAFRIALTYRIREGAVSVEAAVENP
                     GEEPLPFHLGYHPYFRVPIRPDGDRTRCLMRVPSFATWELENLCATGRQVPIDPREAF
                     EPERALGDETMDRVFAALRPDEGGQVRCWVHDPETGYAIEVEFDAREFPVFVVFTSPE
                     APYACLEPWTGLPGGLGDDTPSGQRAQRVPPGGRVENTVRVRVRRA"
     gene            4460..5983
                     /locus_tag="PLY56_02810"
     CDS             4460..5983
                     /locus_tag="PLY56_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOJ20439.1"
                     /translation="MPESPASFGAINYAVLAVYFTGMLAIGIWAGRQTKGAAGYFIGE
                     GKVHHVLVGLSLLGTYLSALTMMALPAMSYGRDDWTYTIQLPFLLVTAVVITGFVLPR
                     LREEGCISVYEFLERRIHVSVRLVASIAFILLSIGRMGLVLYLPSLALSYVLGVNLVA
                     SIVVMGIIVTIYTVVGGIKGIIWVDSIQVVIFIIGAIATLFFAFAPGVDFFAVAHEHG
                     KFRTWVGGWNIAEVTSLWLILETLFQTIRIYATQQDMTQRYMAAESTKKANQSVWLSI
                     LGYIPLAYLFYFIGTALFVYYTVNPDPGVERLVADKKLDALYAYFVVTHMPAGLAGLV
                     IAAFVAAAQSTISSSMNSSSAVCVEDFYRRFWAKSKSDEHYMRVGKALSLVWGVAHIV
                     MAILFMQIERAQVVWQKVMGISTCGVLGLMALAFLPWKVNPWAALTGWVASVISLVVM
                     MFFLQVTPSVALVYPLESNTGINWLLWPVITNLICFGVALGLDRLFPRREKSLAPEG"
     gene            6297..>7621
                     /locus_tag="PLY56_02815"
     CDS             6297..>7621
                     /locus_tag="PLY56_02815"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ20440.1"
                     /translation="MALVSPILRSDRSRHGRWLPALLLLAVLLASRPAGAEGFSFLHL
                     SDVHAPMAASRETIAQARDSGPIRLAPYGVTAPPPSFAIVTGDVTEFGGGAGAWETYR
                     SYWEGARYPVYHVPGNHDNTWWLLRPAIRALHGGLPYAFDYGGCHFIALDSAGRQDPR
                     PGFGVEELEWLRRHLEEVKPGTPLFLFFHHPLSGNEWASPRDYERLLEILRPHHVALM
                     LVGHGHGFRHMRFGGYDAVMGGSTFDKNAKPMSGGNAGYNVVTVQDGVLRVAYRRAAE
                     AEATVPLLEKPLAAPEPLPEVRFVSPEEGTRVSGEAVRVLLSFPDRDGMTGEVALDDG
                     PAIALAPSDEKAGQQGDRLWKATLDLAEATPGVHVLRARFQVGDRHVYAGREILLERP
                     GGPRARWRVNLGASVRGRVAASEGRLYIGTQDGWLVALDAAQGHEVWRFR"
BASE COUNT         1204 a         2405 c         2632 g         1380 t
ORIGIN      
        1 gccagatcgc ccaccgaaac gggggcgtag ccattcatct cgctcatcgt gcccccggca
       61 tcgcagtgaa gtaggccgac gcgcaggggg gcgacttcca gcgccgccgc gtgcgcttcg
      121 aggccggcca ggggatgcgc cagattgagc gcctggctga agctacgccc gaccaccgtg
      181 cacgcgggcg cgccctcctt ttcgattacg ccggcgcccc attccgcgtc gaagagcgtg
      241 cagccttcgg ggagcgagac gtggcggcgc cagagcgacg ggtcatcgat cgggtcgtga
      301 ttcccggcga tgatgaagca ggggatcccc tcccggcaga gcgttttgag tgcctcgccg
      361 aaacgcatcc gcgcccgcag gctgggcgcg gtttcatcga agatgtcgcc gccaaaggtg
      421 acggcatcga gccggccact cagtttctgc tccagggcga aagcgacagc ccggtcgagc
      481 gcgttcaggg tggcatctcg cagacgagtg gccagtgctt cgtctgtttc cgccagcccc
      541 ttgaatggcg tgtcgaggtg caggtccgca aggtggagaa gggtaaccat cgagcctccg
      601 attagaagcc gcttcccctg ccagcgtttc tctgacaggc gcgggcatag cagtcaccct
      661 gttgttcgtg ggagcggcgc gaactcctcc ccggtatcta ggattcggga agcgttgcgc
      721 aagagaggag ttcgcggggc cagggtggaa ggatcgggcg agggatgcac gaaccgctcc
      781 ctgtcgcttc atggcgcgtg gcagtgcagc ccggcgacga tgaggcagga aggtttggcc
      841 cagagccacg ctgttgtctg gtttccctgg caggggactg aagcggcggt ctgcccctcg
      901 attggccgcg tcccgggaag ggcgagaggg gagcccgatg gtcaagagaa aggctggtgt
      961 tccgtgcgga gtataatcct tttctgtgcc gcgctgcttc tagcagcgcc gggaatggga
     1021 aggtgtgccg tggtcaagga tttgtttgac gcgcaggcca ccggcccgaa tctgctcagc
     1081 gacgacaagt ggcagccctg ggagcagggc ttcgagcggc aggggggcgt cttcgtctgc
     1141 gacaacggca acgataacac ggttcaccgc ggcgtggggc agacggtgga gctgaaccag
     1201 gatgtgccgc gaccgatcat cgcggtggcg tggagcaaag ccgagggcat tagcggcggg
     1261 cgcgacaaca actactcgct ctacctcgac ctgctctatg ccgatggcac gccgctgtgg
     1321 gggcagacgg cgcccttcag cgtcggcacg cacgattggg agcgtcggca ggtggtgatt
     1381 ttcccgagca agccggtgaa gcaggtttcg ttctacatgc tgttgcgccg gcacaccggg
     1441 aaggcatcct tccgcgatcc ggtgctgtac cagcttgaga cgccgaaagg cgccgcgatc
     1501 ttcgacgggc ttcccgtcac ggtcaagggc cgcctggcag agggcttcca ggtgcgcgac
     1561 gtcgctgcca acacgggctt ctccaccttt gaggggggca aggcgttcgg cctgaccctg
     1621 gagagccagc gacagcaaag agacggcgcc actttcatcc gctgccggat cgcggatacg
     1681 acgggcaaag accgcgcgat tacgctggtc tacacggtgc cggtggggaa ccagggctgg
     1741 cggtggctgg ccgacccgcg gcggagcctg gagacgcagc cgcccgtgga gtatcacgag
     1801 gtctcccggt ttgccgccgg ggccatcggc gcgctctctc gctatcctct ggcggcgatc
     1861 gcgaggggga acacgggccg ggcgatcggt ctcgatatgg gctatccggc gttctaccgc
     1921 gtgggctata gcgccggcac gggcgagctt ttcatcgcct atgatctggg gctgacgccc
     1981 gagaagccct cggccgagct ccagttctgc ctctacgact tcgatgccgc ctgggggttc
     2041 cggagcgcgc tcgatcgcta ctatcggctc ttccccgagc tcttccgctg ccgcacgccg
     2101 gagcaggggc tctggatgcc atttgccaag atcagtcagg tggaaggctg gcaggacttc
     2161 ggcttcaagt tcaaggaggg gaacgatgag acggcctggg atgatgcgaa caacatcatc
     2221 accttccgct atacggagcc gatgacgtgg tggatgcgga tgccggagga gatgccgcgc
     2281 acgatggagg cggcggtggc cgaggcgaag cgcctggccg gcgaggggaa ccggcaagca
     2341 caggccctcc tggcgagcgg ctaccacgac accaacgggc agttcgtcgc ccggttgctg
     2401 gatacgccgt ggtgtaacgg cgctgtctgg agcatgaact ccgcgccggg tgtgccgggc
     2461 gagacgaccg acttcctgaa caagtggagc ccgaagctcc gcgaacagct gtacggcccc
     2521 gagcgcaagg gcgacctcga tggcgagtac atcgactcca gcgaggggta tgtgaccgac
     2581 gagctgaact tccggcgcga gcactttgcc ggcatgaaga cgccgctcac cttcgatatc
     2641 ggttcgcatc gccccgcgat cttccgcggg ctgatcgcct acgagtacgc tcgcgccatg
     2701 gccgaggacg tgcatcgcat gggcaagctg atgatggcca acagcacgcc gagccggctg
     2761 ccctggctag ctgcctatat ggacgtactg ggcaccgaga ccaactggaa cccacgtggg
     2821 aactggcagc cgatgtctga cgccgacctg ctctatcggc gggcgatgtg cgccaccaag
     2881 ccctactgct tcctgatgaa caccgacttc aacctcttct cgcacgagtt ggtagagaag
     2941 tacatgaagc ggtcgctcgc ctacgggatg ttccctggct tcttcagcgc cgatgcttct
     3001 acgaatcact acttccgcaa ccccgcgctc tataaccgcg accgcccgct cttcaagaag
     3061 tacgtgccgc tgtgcaagcg ggtggcggaa gccgggtggc gcccgatcac cggggcggtg
     3121 acgagtgatc cccaggttta cgtggagcgc tttggagacc gctacctgac gctcttcaac
     3181 gacagctccg aggagaagcg gtttacggtg acgcttcagg gcaaggcccc ggcagccagc
     3241 cgtgacttgg tcaccggcca ggaagtccgc tggcgagcgg gcggcactct ccacggccag
     3301 cctgccgcgc gcgcggagtt cacgctgaag gccgaggatg tggcggtgat cgagttctga
     3361 gccctcgcga gggcaagttg gtgaaaacgg cgcgggccgt gaggcccgcg ccggctggcc
     3421 cccgcctatc cggtgggaga ggcgcggggc ggctagggag gtggcgatgt cacactaccg
     3481 gtgcgcagag ggccggctgg ccacggggga gcgtgagttc cggctcgtgg acgaggcggc
     3541 tggcgtcgat atcacggtga tcccggatca gggcgcggtg tggtccgctt ttggcgcgcg
     3601 cgacgagagc ggcaatcgaa tggagctgtt ccatccggcg gagcacttca cctatccggg
     3661 tgggtctccg gtccttttcc cggtgaccgg gcgctcggcg tgcgacggcg agttgggctt
     3721 ctaccgccat cggggcacgc gctatccgat gccgatccac ggtttcgcgc gcgacctccc
     3781 ctgggatgtg gaggatgtgg ccgcggatga ggccggcgcg cgaatcacct gctcactcca
     3841 agacaccgag gagacacgcc gtgtctaccc ctatgccttc cggattgcgc tcacctaccg
     3901 gattcgggaa ggggcggttt ctgtcgaggc tgccgtggag aatcctggcg aggagccgct
     3961 gccgttccac ctgggctatc atccctactt ccgcgtgcct attcgccccg atggcgaccg
     4021 cacgcgctgc ctgatgcgcg tgccctcgtt cgcgacctgg gagctggaga acctgtgtgc
     4081 cacgggccgg caggttccga tcgatccccg agaagcgttc gagccggagc gcgccctggg
     4141 cgacgagacg atggaccgtg tcttcgccgc gctccgcccg gacgaagggg gccaggtccg
     4201 ctgctgggtg cacgaccccg agacgggcta cgccatcgag gtcgagttcg acgcgcgcga
     4261 gtttcccgtc ttcgtggtct ttacctcgcc ggaggcgccc tacgcctgtc tggagccgtg
     4321 gaccggcctg ccgggcgggt tgggcgacga cacgccgtct gggcagcggg cgcagcgggt
     4381 accgccgggc ggccgggtgg agaacaccgt ccgcgtgcgc gttcgccgcg cgtaaacggc
     4441 agaggggaga agaggaacca tgcctgagag ccccgcgtct ttcggcgcga tcaactacgc
     4501 tgtgctggcc gtctacttca cgggcatgct tgccatcggc atctgggcgg gccggcagac
     4561 gaagggcgct gccggctatt ttattggcga ggggaaggtt caccacgtcc tcgtagggct
     4621 atcgctcctg ggcacctacc tttcggcgct cacgatgatg gcgctcccgg cgatgtcgta
     4681 tggccgcgac gactggacct atacgatcca gctccccttc ctgctcgtca cggcggtcgt
     4741 catcaccggg ttcgtgctcc cgcggctgcg cgaggagggg tgcatcagcg tctacgagtt
     4801 cctggaacgc cggatccacg tttcggtgcg cctggttgcg tccatcgcct tcatcctcct
     4861 ctccatcggg cgcatgggcc tcgtcctcta cctgccgtcg ctggcgctct cctacgttct
     4921 cggggtcaat cttgtcgcct cgatcgtggt catggggatc atcgtcacga tctacacggt
     4981 ggtcggcggg atcaagggca tcatctgggt tgattcgatc caggtggtga tctttatcat
     5041 cggcgctatc gccacgctgt tctttgcctt cgccccgggc gtggacttct tcgctgtggc
     5101 ccacgagcat gggaagtttc gcacctgggt gggcgggtgg aacatcgccg aggtgacgtc
     5161 gctgtggctg atcctggaga cgcttttcca gacgatccgg atctatgcca cgcagcagga
     5221 tatgacgcag cgctacatgg ccgcggaatc gacgaagaag gcgaaccaaa gcgtgtggct
     5281 ctcgatcctg ggttacatcc cgctcgccta tctcttctac ttcatcggca cggcgctctt
     5341 cgtgtactac acggtgaacc ctgatccggg cgtggagcgg cttgtggccg acaagaagct
     5401 ggacgcgctt tatgcctact tcgtggtgac gcacatgccg gcgggcctgg ccggcttggt
     5461 gatcgccgcg ttcgttgccg cagcgcagtc caccatctcg tcgagcatga actcgagctc
     5521 cgcggtgtgt gtggaggatt tctaccgccg cttctgggcg aaatcgaaga gtgacgagca
     5581 ctacatgcgc gtggggaagg cgctctcgct cgtctggggc gtagcgcaca tcgtgatggc
     5641 gatcctcttc atgcagattg agcgggcgca ggtggtatgg cagaaggtga tggggatctc
     5701 cacctgcggt gtgctagggc tgatggcgct ggcgttcctt ccctggaagg tgaacccctg
     5761 ggctgcgctc accggctggg tggcatccgt catctcgctc gtcgtgatga tgttcttcct
     5821 gcaggtcact cccagcgtgg cgctcgtcta tcctctggag agcaatacgg ggatcaactg
     5881 gttgctctgg ccggtgatca ccaacctgat ctgtttcggc gttgccctcg ggctggaccg
     5941 cctcttcccg cgccgagaga agtccctcgc ccccgagggg tagcgtgttg gaacacctca
     6001 cggcgcggag cttccagggc tgagcagcac ggatacgaat atgcccggcg cgcgactgga
     6061 gacgcgggcc atccggggca cggccatccc cgggggatcg cgaaggaccg cgaagcggtc
     6121 ccacaacata gcccagggtc tcctgaccag accggacctt atctccgaat accaggggag
     6181 cactgcgccc gttttgacgc cggatggccc ctggagtata ataggagcaa tcgtttgaga
     6241 gttctcccgc gggccggttg actcgtgccg cgagtggatc tggaaaggac gcacgattgg
     6301 cgctggtatc tcccatcctg aggtctgatc ggagccggca cgggcgctgg ctgccggcgc
     6361 tcctcctgct ggccgtcttg ctggcctccc gtccggcggg cgccgaggga ttctcctttc
     6421 tccacctgag cgatgttcac gcgccgatgg ctgccagtcg cgagacgatt gcccaggcgc
     6481 gcgattccgg gccgatccgc ctggcgccgt acggtgtgac cgccccgccg ccctcgttcg
     6541 ccatagtcac gggcgatgtg accgagtttg gtggcggtgc cggcgcctgg gagacctacc
     6601 gctcctactg ggagggcgcc cggtatcccg tctaccacgt gcccggcaac cacgacaaca
     6661 cctggtggct gctgcgcccg gcgatccgcg ccctgcatgg cggcctgccc tatgcgtttg
     6721 attacggcgg ctgccacttc atcgccctcg acagcgccgg aaggcaggat ccccggcccg
     6781 ggttcggcgt ggaggagctg gagtggctac gcaggcacct ggaagaggtg aagccgggca
     6841 cgccgctctt cctcttcttc caccacccgc tctccgggaa cgagtgggcg agcccgcgcg
     6901 actacgagcg tctcctcgag atcctgcggc cgcatcacgt ggcgctgatg ctggtaggcc
     6961 acggtcatgg cttccggcac atgcgcttcg gcggctacga cgccgtcatg ggaggctcta
     7021 ccttcgacaa gaatgcgaag ccgatgtcgg gcggcaacgc cggctataac gtggtgaccg
     7081 tccaggatgg tgtcttgcgc gtggcctacc ggcgcgccgc cgaggccgaa gcgacggtgc
     7141 ccctcctgga gaagccgctg gccgccccgg agccactccc ggaggtgcgt tttgtctcgc
     7201 ccgaggaggg cacccgggtt tcgggtgagg cggtgcgcgt tctcctttcg ttcccggacc
     7261 gggatggcat gaccggtgag gttgccctcg atgacggccc ggccatcgcg ctcgcgccgt
     7321 ccgatgagaa agcaggccag cagggcgacc ggctctggaa agcgaccctg gatctggccg
     7381 aggccacgcc gggagtccac gtgctccgcg cgcgcttcca ggtgggcgac cggcacgtct
     7441 atgccggccg cgagattctc ctggagcgcc ctgggggacc tcgcgcgcga tggcgcgtga
     7501 atctgggcgc ttccgtgcgc gggagagtgg ccgcgagcga gggccggctc tatatcggaa
     7561 cgcaggatgg ctggttggtc gcgctggacg cggcgcaggg gcatgaggtg tggcgcttcc
     7621 g
//
LOCUS       DAOSCV010000067         7590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6259_length_7590_cov_3.912807, whole genome shotgun sequence.
ACCESSION   DAOSCV010000067 DAOSCV010000000
VERSION     DAOSCV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6259_length_7590_cov_3.912807"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PLY56_02820"
     CDS             <1..809
                     /locus_tag="PLY56_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOJ20441.1"
                     /translation="REVERMVNERAMADQPVTTVVVDIAEAQQMGAMALFGEKYGEKV
                     RVVRMGDFSMELCGGTHVERTGQIGLFRILSESSVGSGLRRIEAVTGHGVIEHLEAQE
                     SLLRDAAQALNTRPAEVPARIAAQADQIRELERRLQELQQQQAGSQAEALLSQAVEVN
                     GVKLLATPAGEADRDALTRMADTLADKLRSGVVVLAGLSNNTVIFVAKVTPDLVKRGA
                     HAGNLVREVAKVAGGGGGGRPDFAQAGGKDPSKLESALGKAAEVLAAQLK"
     gene            1168..2259
                     /locus_tag="PLY56_02825"
     CDS             1168..2259
                     /locus_tag="PLY56_02825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ20442.1"
                     /translation="MNDASNRHAPPRGYSIPLVDLAEEKSRQVVVDREPGQYLGHPST
                     VLLRDGKTILVAYPKGHGRGAIVLKRSTDGGRTWSERLPVPENWATSLETPTLHRVCD
                     AEGHERIILFSGLYPIRMAVSEDEGHTWTPLRPIDNFGGIVAMGDVIRLKSGAWMALF
                     HDDGRYLDGAGQPGAFHVYKILSRDGGLTWSAPEVVTTHPSAHLCEPGAVRSPDGSQI
                     ALLLRENSRKHNAMIVFSEDEGESWSEPVELPGSLTGDRHVARYAPDGRLVVVFRDMA
                     HETPTWGDFVAWVGTYDDLVTGREGQCRIRLLDNTKRADCGYAGLEVLPDSTLVATTY
                     YHFEEREPPIVCSVRFTLAEIDARLAAQA"
     gene            complement(2415..5000)
                     /gene="glgP"
                     /locus_tag="PLY56_02830"
     CDS             complement(2415..5000)
                     /gene="glgP"
                     /locus_tag="PLY56_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ20443.1"
                     /translation="MRPIRTFTVVPSLPASLSRLRDLAYNLWWSWDHEAVDLFRRLDE
                     DLWESTGHDVVKMLGSISQERLEAAAADQGFLAHMNRVLSRLDHYLKAGDTWHEMISR
                     MAQGGTGCQFPHCVAYFSMEFGISECLPNYSGGLGMLSGDHLKSASDLGLPLVGVGLL
                     YQEGYFRQYLNLDGWQNERYPHNDFYNMPITLARGKDGNPITISVVYPGRLVKAQIWR
                     VQVGRVPLFLLDTNLPENSREDQDVTDRLYGGGTDVRIRQEILLGIGGMQALRALGLT
                     PVVCHMNEGHAAFLGLERIRLLRHEHGLSFDEAREVAAAGHVFTTHTPVPAGIDRFPP
                     DLVHAYFEHDYEQLGLTRREFLGLGRVNPADEGEAFCMAVLALRLSSAANGVSKLHGQ
                     VSRKMWAGMWPGVPADDVPITSVTNGVHLQSWVSRDLASLYDRYLGPGWRENPADPRI
                     WAEVQDIPAEELWRTHERRRERLVAFARSRLRQQLIQRGASQQEIAAAAEVLHPEALT
                     IGFARRFATYKRASLLFRDCERLARMVSSRERPVQFLFAGKAHPDDHPGKELVRELVH
                     HIQEPRFRPYMVFLEDYDIIVARYLISGCDVWLNTPLRPREASGTSGMKAAANGVLNC
                     STLDGWWCEAYDLSTEVGWAIGRGEEYDDSRYQDEVEAGALYNLLEKEIVPLFYERGP
                     DNLPRRWISRMKASISQIAPFFNTHRMVSEYATELYFPAAQRATRLAENGFARAKALA
                     AWKDRVRAAWSDVRVLDITAPDGEELPVGRDLKVKVKVHLGRLTAEDVCVELYQGRVN
                     EHMEIADPEAIRMEFTGEATGGVFEFAGAIPCRSSGYHGFAVRVVPSHPDLANPFELG
                     LIAWS"
     gene            complement(5111..5959)
                     /locus_tag="PLY56_02835"
     CDS             complement(5111..5959)
                     /locus_tag="PLY56_02835"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOJ20444.1"
                     /translation="MMILPDTHTHTRWSDGVGEIEANVRRAAELGLPAIACTDHMPLQ
                     SPPPGLTRPEDAPPGRQTTWHMAWQDLPTYIADVERARAAYPQVEVLVALEFDYWPGV
                     EPVIEELSRVYPWDLRLGSVHYVGDFGIDSDEEIPRWERSDPDAVWEAYFALLTEAAS
                     SGLFDLIGHADLVKKFGFLPRRDPGSWYDAFLTAAAASGVAIELNTAGLRKPCAEIYP
                     GLAFLRAARAHGVPIAFGSDAHQASQVAAGFDAAVALARSAGYEEYVRFRPGRKREVC
                     PLPTVL"
     gene            6129..6521
                     /locus_tag="PLY56_02840"
     CDS             6129..6521
                     /locus_tag="PLY56_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20445.1"
                     /translation="MRVVFEDYWRDLREGMEREGIPLPPPDLEAFIEELREQGVAEVR
                     LCMRWLHVLNVEASGGSLPALDALARSPYMHFGTYLLATARTPDGILFEYRQRLYPED
                     GQPHELLEQARSRLEEAGFRVLNGRWQV"
     gene            6563..>7590
                     /locus_tag="PLY56_02845"
     CDS             6563..>7590
                     /locus_tag="PLY56_02845"
                     /inference="COORDINATES: protein motif:HMM:NF021041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOJ20446.1"
                     /translation="MGRLMHLMAALLVMSLATPACGAEDLAARARRSLERGVEFFTTK
                     VATQGGYLWWYSEDLQERWGETQATASQVWVQPPGTPAVGEAFLRAYRATGDRRYLEA
                     ARAAARALTWGQLESGGWDYRIDFDEKESQRWYFRRDKGKLSPEEAAKRRNTSTFDDD
                     NSQSALRFLMGVCQETKDAEIQDALDYGLAFLLKAQYPNGAWPQRYPPPKSGYGSYYT
                     FNDDTIRDCVRTALAAHRHTGRAEFLEAAKRAGEFILKAQQPEPQPVWAQQYDREMKP
                     AWARKFEPPSVCSSESVGVMHTLIDLYIETGEERFLEPIPRALAWFERSQIAPNRWAR
                     FYELETNRP"
BASE COUNT         1310 a         2561 c         2518 g         1201 t
ORIGIN      
        1 tgcgcgaggt ggagcgaatg gtgaacgagc gcgcgatggc cgatcagccg gtgactaccg
       61 tggtggtcga tatcgccgaa gcgcagcaga tgggcgccat ggcccttttt ggcgagaagt
      121 acggcgagaa ggtgcgcgtg gtgcgcatgg gcgacttctc gatggagctg tgcggcggca
      181 cgcacgtgga acgcaccggg cagatcggcc tcttccgcat cctgagcgag agcagcgtcg
      241 gctctggact ccgccgtatc gaggcggtga ccggccacgg cgtcatcgag catctggaag
      301 cccaggaatc gctgttgcgc gatgccgccc aggcgctcaa cacgcgtccc gctgaggtgc
      361 ccgcacggat tgctgcccag gcggatcaga tccgcgagct ggagcgccgc ctgcaagaac
      421 tgcagcagca gcaggccggc tctcaggccg aggcgctcct gtcccaggcc gtcgaagtga
      481 acggcgtgaa gctactcgcc accccggcgg gagaagcgga ccgcgacgcg ctcactcgga
      541 tggcggatac cctcgccgac aagctgcgct ccggcgtggt cgtgctggcc ggcctctcca
      601 acaacaccgt gatcttcgtt gccaaggtaa cgcccgatct ggtaaagcgg ggcgcgcacg
      661 cgggcaacct ggtccgcgag gtcgcgaagg tggccggcgg cggcggcggc gggcggcccg
      721 atttcgccca ggccgggggc aaggatccgt cgaagctgga gagcgccctg gggaaggccg
      781 ccgaggtcct ggcagcccag ctcaaatagc gcggaacggg gagagcgtgc gcaaggtacg
      841 cggggctccc cgcccgtgga tgccgggcat cacgcctcgc gcgtatcgcg cggcgctaga
      901 gcaccgcgct gcttgcgagc gaaaccctat cgggctgggc tgcaccgggt gccctatagc
      961 ggcggcggct gggaggggag ccccgaaggg gctttgctga gaagcagcgc ggagcttcca
     1021 gctccgcgcg ggcgtcgtgc aacccatggc gcggccctct ccatccgcgc ccgtgctctt
     1081 catccgtggt atcgcggggc cgggggcccg tacaccccgg ccccgcgccg gatcggaccg
     1141 cgtccgccag ctggaaggag ggtgaccatg aacgacgcga gcaaccgcca cgcgccgccg
     1201 cgcggctact ccattcccct cgtcgatctg gccgaggaga agtcacgcca ggttgtggtc
     1261 gaccgagagc caggccagta cctggggcac cccagcacgg tgctcctccg ggatggaaag
     1321 acgattctgg tcgcctaccc gaaagggcat ggccgcggcg ccatcgtgct taagcggagc
     1381 accgatggag ggcgcacctg gagcgagcgc ctccctgtgc ccgagaactg ggccacttcg
     1441 ctcgagacgc ccacgctgca ccgcgtctgc gatgcggagg gccacgagcg catcatcctc
     1501 ttctccggcc tctatcccat ccgcatggcg gtctccgagg atgaggggca cacctggacc
     1561 cccttgcgcc ccatcgacaa cttcgggggg atcgtcgcca tgggcgatgt gatccgcctg
     1621 aagagcggcg cgtggatggc gctcttccat gacgacgggc gatacctcga cggcgcgggc
     1681 cagccggggg cgtttcacgt ttacaagata ctatctcgcg atggcgggct gacctggagc
     1741 gctccggagg tcgtgaccac ccatccgagc gcacacctgt gcgagccagg cgctgtgcgc
     1801 tcgcccgatg gaagccagat cgccctcctc ttgcgcgaga atagccgcaa gcacaacgcg
     1861 atgatcgtct tcagcgagga cgagggtgag agctggtcgg aaccggtgga gctgccgggc
     1921 agccttacgg gcgaccggca cgtcgcccgc tacgcgcccg atggccgcct ggtcgtcgtc
     1981 tttcgcgaca tggcgcacga gacgcccacg tggggcgact tcgtggcctg ggtaggcacc
     2041 tacgacgacc tggtgaccgg ccgggagggc cagtgccgca tccgcctcct ggataacacc
     2101 aagagagcgg attgcgggta tgcgggcctg gaggtactcc cggatagcac cctggtcgca
     2161 accacttact accactttga ggagcgagag ccgccaattg tatgtagcgt gcgcttcacc
     2221 ctcgccgaga tcgatgcccg gttggcggcg caagcctgaa gcgccctgcc tggccatccc
     2281 ggatcgcagg agcgcctgag gccggcgggt gggaggaagt cgcccccggg ttctattcgc
     2341 cggtccttcc aggctggcgg ctggccgcca gcctggaagg cggatactct cgcctgtcgg
     2401 ggagacgcgg ccagctatga ccaggcaatc aaccccagct cgaaggggtt ggccagatcc
     2461 ggatggcttg gcacgacgcg cacggcaaag ccgtggtagc cgctggagcg gcacggaatg
     2521 gcaccggcaa actcgaacac ccctccggtt gcttctccgg tgaactccat ccggatcgcc
     2581 tcgggatcgg cgatctccat gtgctcattc acccggccct gatacagctc cacgcacacg
     2641 tcttcggccg tcagccggcc caggtggacc ttcaccttca ccttcagatc gcgcccgacg
     2701 ggaagctctt cgccgtctgg cgccgtaata tcgagcacgc gcacatcgct ccaggcagca
     2761 cgcacgcggt ctttccaggc ggcaagcgcc ttcgcgcggg cgaagccgtt ttccgccagt
     2821 ctggtcgcgc gctgggcggc ggggaagtag agctccgtgg cgtactcgct caccatgcgg
     2881 tgagtgttga agaacggcgc gatctggctg atgctggcct tcatccggct gatccacctc
     2941 cgtggcaggt tgtcggggcc gcgctcatag aagagcggca cgatctcctt ctccaggagg
     3001 ttgtagagcg cgccggcttc cacctcgtcc tgatagcggg agtcgtcata ctcctcgccg
     3061 cgcccgatcg cccatcccac ctcggtgctg agatcgtacg cctcacacca ccacccatcc
     3121 agcgtcgagc agttgaggac cccattcgcg gccgccttca tgccgctcgt tccgctcgcc
     3181 tcgcgcgggc gcagcggggt gttcagccag acatcgcacc ccgagatgag gtaacgggcc
     3241 acgataatgt cataatcctc caggaagacc atgtagggcc ggaagcgggg ctcctggata
     3301 tggtggacga gctctcggac cagttccttg cccgggtggt catctgggtg cgctttaccc
     3361 gcgaagagga actgcaccgg gcgctcgcgg ctgctcacca tgcgcgccag gcgctcgcag
     3421 tcgcggaaga ggagcgacgc gcgcttgtag gtggcgaagc ggcgggcaaa gccgatggtc
     3481 agtgcctccg ggtggagcac ctccgccgcc gccgcgatct cctgttgcga tgctccgcgc
     3541 tggatcagct gctggcgcag ccggctccgc gcgaatgcta ccaggcgctc gcgccgccgc
     3601 tcgtgcgtgc gccacagctc ctcggccggg atatcctgaa cctccgccca gatgcgcggg
     3661 tcggccgggt tctcgcgcca accggggcca aggtagcgat catagaggct cgcgaggtca
     3721 cgcgataccc acgactggag atgcacgccg ttcgtcaccg aggtgatcgg cacatcatcg
     3781 gcggggaccc caggccacat gccggcccac atcttccgag acacctggcc gtgcagcttg
     3841 ctcacgccat tcgcagccga ggagagacgg agcgcgagca cggccatgca gaacgcttcg
     3901 ccctcatccg ccgggttcac gcggcccagc cccaggaact cccggcgcgt gaggcccaac
     3961 tgctcgtagt catgctcgaa gtaggcatga accaggtcgg gtgggaagcg gtcgatcccc
     4021 gcgggcaccg gcgtgtgcgt ggtgaagacg tgcccggcag cggccacctc gcgtgcttcg
     4081 tcgaaggaaa gtccatgctc gtggcgcagg aggcggatcc gctccaggcc caggaacgcg
     4141 gcatggccct cattcatgtg gcagacgacg ggcgtcaggc caagagcgcg cagcgcctgc
     4201 atccccccga tcccaagcag aatctcctgc cggatgcgca catccgtccc gcccccgtag
     4261 aggcggtcgg tgacatcctg atcctcgcgc gagttctccg ggagattcgt atccagcagg
     4321 aaaaggggca cgcgccctac ctgcacgcgc cagatctgcg ccttgaccaa ccggcctggg
     4381 taaaccacag agattgtgat cgggtttcca tctttgcccc gcgcgagggt aatcggcatg
     4441 ttgtagaagt cattatgagg atagcgctcg ttctgccagc catcgagatt gaggtattgc
     4501 cggaaatagc cctcctggta gagcaagcca acgcccacca gcggaaggcc cagatcactc
     4561 gcggatttga gatggtctcc ggagagcatc cccagaccgc ccgagtagtt gggcaggcac
     4621 tcgctgatcc caaactccat ggagaagtag gccacgcagt gaggaaactg gcagccggtc
     4681 ccgccctgcg ccatgcggct gatcatctca tgccaggtat cgccagcctt caggtagtga
     4741 tccaggcgag agaggacccg gttcatatgc gccaaaaagc cctgatcggc agcggcggcc
     4801 tccaggcgct cctgtgagat gctcccgagc atcttcacca catcgtggcc cgtgctctcc
     4861 cagaggtcct catccagccg gcggaagaga tccacggctt catgatccca cgaccaccac
     4921 aggttatagg cgagatccct caagcgcgaa aggctcgcgg gcagggacgg caccacggta
     4981 aaggtacgta ttgggcgcat gtgctcatcc ttcctggctg agtcgatcgg tggaacgacc
     5041 ggcgacaagg actgcgcggg gttcgccgcc ggggcggatc cagaacgatc ttacccagat
     5101 ccgcgcgaaa ctatagcacg gtaggaaggg ggcacacctc gcgcttccga ccgggccgga
     5161 agcgaacgta ctcctcatag cctgccgacc gcgccagggc cactgcggca tcgaagccgg
     5221 cggccacctg gctggcctgg tgcgcatcgc tgccaaaggc gatcggcacg ccatgggccc
     5281 tcgctgcgcg gaggaacgcc aatccagggt agatttctgc gcatggcttg cgcagccccg
     5341 cggtgttcag ctcaatcgcc acgccgctcg cggcggcggc agtcagaaac gcgtcatacc
     5401 acgagcccgg atcgcgccgg ggcaggaagc caaacttctt caccagatcc gcgtggccga
     5461 tgagatcgaa caggccgctg ctggccgcct cggtcagtag cgcgaagtag gcttcccaga
     5521 cggcatccgg gtcgctgcgc tcccaccggg ggatctcctc gtccgaatcg atgccaaaat
     5581 cgcccacgta gtggacgctg cccaaccgga gatcccaggg gtacacgcgc gagagctcct
     5641 cgataacagg ctccacgccc ggccagtagt caaactcaag ggcgacgagc acctccacct
     5701 ggggataggc tgcacgcgcc cgctccacat ccgcgatgta ggtcgggagg tcctgccacg
     5761 ccatgtgcca ggtggtctgg cggcccggtg gcgcatcctc cgggcgcgtg aggcccgggg
     5821 gcgggctctg cagcggcata tggtcggtac acgcgatggc cggcaggccc agctcggctg
     5881 cgcggcgcac attcgcctcg atttcgccga cgccgtcgct ccagcgtgtg tgtgtgtgtg
     5941 tgtccggcag gatcatcata ctctcttgct tccatgcgcc ggcccccatc cccttccccc
     6001 ccagccgact tcggccggct ccgctcattc cgcgcgcccg cctttccacc cgcgcgagtt
     6061 gcgagagacg gcgctcccgg caggagagcc ttcagccgcc gcgaatcacg cgcaagggga
     6121 ggtgcgagat gcgcgtcgtt ttcgaagact actggcggga tctgcgcgag gggatggaac
     6181 gagagggcat ccccctgccg cccccagacc tggaggcgtt catcgaggag ctacgcgagc
     6241 agggtgtcgc cgaggtgcgt ctctgcatgc gctggctcca cgtgctgaac gtcgaagcat
     6301 cgggcggctc tctgcccgcc ctcgatgcgc tggcgcggag cccctacatg cactttggca
     6361 cctacctcct ggccaccgcg cgcaccccag atggcatcct cttcgagtat cgccagcgcc
     6421 tctaccccga ggatggccaa ccgcatgaac tcctggagca ggcgcgctcc cggctggagg
     6481 aggccgggtt tcgggtcctc aacggccgct ggcaggtctg aatgccggat ggccctggcg
     6541 atgggccgga ttggaggaga cgatgggccg actgatgcac ctcatggccg ctttgctggt
     6601 catgtctctc gcgacgcccg cgtgtggcgc cgaggatctg gctgcacgcg cccgccgatc
     6661 actggagcgc ggcgtggagt ttttcacgac gaaggtcgct acccagggcg gctatctgtg
     6721 gtggtactcc gaggatttgc aggagcgctg gggagagact caggccacgg cgtctcaggt
     6781 ctgggtccag cctccgggca ccccggcggt gggcgaggca ttcctgcgcg cctatcgcgc
     6841 cacgggcgac cggcgctacc tggaagccgc gcgcgcggcc gccagagcgc ttacctgggg
     6901 gcaactcgag tcgggcgggt gggactaccg gatcgacttc gacgagaagg agagccagcg
     6961 ctggtacttc cggcgcgaca aaggaaaact ctcgcctgag gaagccgcca agcggcgcaa
     7021 cacctccacc tttgatgacg ataactccca gagcgccctc cggtttctga tgggcgtctg
     7081 tcaggagacg aaggatgccg aaatccagga tgcgctcgat tacggcctcg ccttcctgct
     7141 gaaggcgcaa tatcccaacg gcgcctggcc acagcgctac ccgcccccga agagcggcta
     7201 cggctcctac tacaccttca acgacgacac gatccgcgat tgcgtccgca ccgccctggc
     7261 cgcgcaccgt cacaccggcc gggccgagtt cttggaagca gcgaagcgcg ccggggagtt
     7321 catcctgaag gcgcagcagc cggagccgca gccggtatgg gcccagcagt acgaccgcga
     7381 gatgaagcca gcctgggcgc gcaagttcga gccaccctcg gtatgcagtt ccgagagcgt
     7441 gggggtgatg cataccctca tcgatctcta cattgagaca ggggaagagc gcttcctgga
     7501 gccgatcccg cgcgcgctcg cgtggttcga acgctcgcag atcgcgccga accgctgggc
     7561 acgcttctat gagctcgaga ccaaccggcc
//
LOCUS       DAOSCV010000068         7589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6261_length_7589_cov_3.657154, whole genome shotgun sequence.
ACCESSION   DAOSCV010000068 DAOSCV010000000
VERSION     DAOSCV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6261_length_7589_cov_3.657154"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="PLY56_02850"
     CDS             <1..765
                     /locus_tag="PLY56_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20447.1"
                     /translation="KKAADFLATHATAESDGIPDVIRHVRLHARPDGSVQQQVYYQEG
                     RKCDVYLGRALAGLAYFAYALQVSGEKVPERYWRVMDQTVTWSMKKMKPDTGWFDWQC
                     EDIVEGGCHTFLGNIYIGEGLFGCYLASRSAGRAAQAAAAAKAAHLAYRYVTDHCTIR
                     GQRYKPPLEFWVGPYVYWLFTEYHDAVGKDPAFQEWLETLHRQWAVDREWRDFLARDE
                     KAGHRTSTNGALEISILGYLGLRHLEEIGKPYHLPR"
     gene            complement(889..1977)
                     /locus_tag="PLY56_02855"
     CDS             complement(889..1977)
                     /locus_tag="PLY56_02855"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOJ20448.1"
                     /translation="MDAQVSQAPLVYIGTYTANSGSEGIYVYRFDRATGELTFTGHTG
                     KVANPSYLAIHPDGRHLYSVCEVGTFEGKKGGGVSAFRIDRRTGELTLLNQEPSSGGA
                     PCYISLGKRGRHALVANYSAGSVGVLPIREDGTVAPLSHVVQHEGRGANPDRQEGPHA
                     HCIRMDPAGRFVFAVDLGIDKVVIYRLDETTGKLVPNDPPFAQFHPGAGPRHIVFQPD
                     GKQAFVVNELDSTLTRCAYDASTGALKPVQMLTTLPEEFTDNNTGADVHVHPSGRFVY
                     SSNRGHDSIAIFAIDASGALVPVGHESTRGKEPRGFAIDPMGNFLLAANQNTNTVVTF
                     RIDTRTGKLTPTGHQVSVPKPVCIQFAW"
     gene            complement(2033..5518)
                     /locus_tag="PLY56_02860"
     CDS             complement(2033..5518)
                     /locus_tag="PLY56_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ20449.1"
                     /translation="MAILRALLVVTASLMVVAASAAPVPNASFEEGTDRPTAWSPSDS
                     TAAWEEGGRTGRRCLVINGDGKESHFWKCDNWLPEPGGIYRLTFWARSEGATGGCVVS
                     GLNIANQDFPLPGKEWVRYTRVFMAPRSVQGAYLRLGTWSTTGKVLFDDVTVTPVLPL
                     VGQLGEGERVSQGRYIFQAPLQGEGSNYSRPLVGFTAGFNTNRWVLSDGALITYRHAL
                     PGAKQRAATIHANVGYHVAGRLLVDASRDGKTWREVGALGEKGALKAELPADLFPADE
                     IQIRLRGEGEKRSGVDSHPGSFQIHDYTYEAELEAAPRDLAGQTSYLEIEQERKPSVV
                     TVESLGGLRPGVDPNVRLRVSNPGRAALPVTAVFAVSSERGKPQQFTTKMNVPAGGEA
                     PLLLPYTLRETGRMQATLRVTAGREVLLAGKTEFDVPALYAADFGYLVSQDATAAVWW
                     AEPTHKVSRDRPAPETRQSVVRLSAAGHEYEPFQVVLRPTQTLRGVTASATDLDGPKG
                     ARIDASNIRIHLVEYVRVERPTDRAGTPGLWPDPLPPVEGPFDVAANTNQPLWVTVYV
                     PAGTPAGDYRGALQLAAGPWRQEVPVQLRVWDFTLPKETHVKTAFGLNTATIRSYHNL
                     ETQEELAKVFDLYLQNFAAHRISPYDPMALAPIRVSFGETTWDGGQQVMEQPFEGKRC
                     LKVVDDSDRSSIGASYRNRVTVDPEAEYILSWAARTEKDGQTYLITLQNYDATGAWMS
                     GRNIDLRFRGSTTWKQESVSLKGRLPKDSRAVQIVLRPVIWSEKGEATGTAWFDALSL
                     RVAGGTELLKDGGFEEVADPLRVKVDFTEFDRAAHRAFNELGFNSFRLHLQGMGGGTF
                     HSRHKGRIGAYEQGTPEYEALFTSYARQLQDHLEEKGWLDKAYIYWFDEPEQRDYDFV
                     KEGMALIKRAAPKLARMLTEQVEPELADYVDIWCPVTSNYDEERSRERQAKGEKIWWY
                     VCTSPKAPYCTLFIDHPGIEMRMWLWQTVKYNVEGILVWTSNYWTSNAAFPPPAVQNP
                     WEDAMAYISGYDYQPGQIGYWGNGDGRFIYPPNRDPANDKRKHLTGPIDSFRWEMLRE
                     GIEDYEYFYLLRETIAQARARGVSANMLAAAEKLLQVPPEVCVDMTTFTLDPAPLYAH
                     REKIARAIESLRKAN"
     gene            5960..6328
                     /locus_tag="PLY56_02865"
     CDS             5960..6328
                     /locus_tag="PLY56_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOJ20450.1"
                     /translation="MHEVGIAEELVRQVVAAACGMARVTEVAVDVGALQWVDSDALRQ
                     AFDAASQDTIAEGARLELREVPPRAVCGACAAVYVPEELGIYVCPACGAAEPEFVEGN
                     DIVLVSLECLSPEEENRESY"
     gene            6315..6962
                     /gene="hypB"
                     /locus_tag="PLY56_02870"
     CDS             6315..6962
                     /gene="hypB"
                     /locus_tag="PLY56_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOJ20451.1"
                     /translation="MSRIEVVSSVLRANQVRAEETRARLDAAGVACVNLISAPGSGKT
                     TLLQKTITALEGIPCAVLVGDLQTTRDAERLAAAGAPVVQINTGRGCHLTADQVAEGL
                     DRLDLSQIQLLFIENVGNMVCPAGVDLGEHRRVALLSVPEGDDKVAKYPTLFQGADLV
                     LLTKVDLLSVLPFAVRQVEEDLARVNTRAPLVRLSTLEGTGMDAWLAWLRALVAG"
     gene            7255..>7589
                     /locus_tag="PLY56_02875"
     CDS             7255..>7589
                     /locus_tag="PLY56_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20452.1"
                     /translation="MPLLATIARVGFAGLLALGMAAPGQIEAAEIRLDQDRLRLQVNE
                     ASGRITRVEASGANLTAPDAVSRSGFALADGPKAECVPVTCRVTPRGAGGLLWDGQAG
                     ALRIACQSLP"
BASE COUNT         1253 a         2442 c         2539 g         1355 t
ORIGIN      
        1 aagaaggcgg ccgatttcct cgccacgcac gccaccgccg agagcgacgg catcccggat
       61 gtgatccgcc acgtccggct acatgcccgg ccggatggca gcgtccagca gcaggtctac
      121 taccaggaag gccggaagtg cgacgtctat ctgggccggg cgcttgccgg cctcgcctac
      181 tttgcctatg ccttgcaagt ctcgggcgag aaggtgccgg agcgctattg gcgcgtgatg
      241 gaccagacgg tcacctggtc gatgaagaag atgaagccgg ataccggctg gttcgactgg
      301 cagtgcgagg atatcgtcga gggcggctgc cacaccttcc tgggcaacat ctacattggc
      361 gagggcctct tcggatgcta tctggccagc cggagcgccg ggcgcgcggc gcaggccgcc
      421 gcggcggcga aagcggccca cctggcgtat cggtatgtca ccgaccactg caccattcgc
      481 gggcagcgct ataagccacc gctggagttc tgggtaggac cctacgtcta ctggctcttc
      541 acggagtatc acgacgccgt aggtaaggat cctgccttcc aggagtggct ggagacgctt
      601 caccggcagt gggccgtgga tcgggagtgg cgcgacttcc tcgcgcgcga cgagaaggcc
      661 gggcaccgca cgtccaccaa tggcgcgttg gagatctcca tcctcggcta tctcggcctg
      721 cggcacctgg aggagatcgg caagccctac cacctcccgc ggtagagcgc ggtcatgcca
      781 cgccgggcga ccggcataga tcgaactgac aggctctctc cccgcgcggg gagagagcct
      841 gtcgcggttt ccaggagttc gatagtgtcc gcgcgcggcc tcagccggtt accacgcgaa
      901 ctggatgcag accggcttcg gcacgctgac ctggtggccg gttggggtca gcttgccggt
      961 gcgcgtgtcg atgcggaaag tgaccacggt gttcgtgttc tggttggcag ccaggaggaa
     1021 gttgcccatc gggtcgatgg caaagccgcg gggctctttg cctcgcgtgc tttcgtggcc
     1081 cacggggacg agcgcgccac tcgcgtcgat ggcgaagata gcgatgctgt cgtggccccg
     1141 gttcgagcta tagacgaacc ggcccgaggg atgcacatgc acatcggcgc cggtgttgtt
     1201 atcggtgaac tcctcgggga gcgtcgtgag catctgtacc ggcttcagag cgcccgtgga
     1261 cgcatcgtaa gcacagcggg tgagcgtcga atcgagctca ttgacgacaa aagcctgctt
     1321 cccatccggt tggaaaacga tatgccgggg cccggccccg gggtggaact gcgcgaaggg
     1381 gggatcgttc ggcacgagct tgcccgtcgt ctcatccaac cgatagatca ccactttgtc
     1441 gattccaaga tctaccgcga agacaaagcg cccggcggga tccattcgga tgcagtgcgc
     1501 gtgggggccc tcctgccggt cggggttggc gccgcggccc tcgtgctgca ccacatggct
     1561 caggggagca acagtgccat cttcccggat ggggagcaca ccgacgctcc ccgcggagta
     1621 gttggcgacc agcgcgtgcc ggccacgttt gcccaggctg atgtagcagg gcgctcctcc
     1681 ggaagagggc tcctggttga gcagggtgag ctcacccgtc cgccggtcga tgcggaaggc
     1741 actcaccccc ccgcccttct tcccctcgaa ggtgccgacc tcgcacaccg agtagagatg
     1801 ccggccatcg ggatggatcg ccagatagga ggggttcgcc acctttccgg tatggccggt
     1861 aaaggtcagc tctcccgtcg cgcggtcgaa ccggtagacg tagatcccct cggacccgga
     1921 gttggccgtg tacgtgccga tatagactag cggcgcctga cttacctgcg cgtccattct
     1981 catcaccctc tctgctggct cctgccagca cggttccggt tgcttgctct ctttaattcg
     2041 cctttcgcag gctctcaatc gcccgggcga tcttctcgcg gtgggcgtaa agcggcgccg
     2101 ggtcgagggt gaaggtggtc atatccacgc agacctcggg cggcacctgc agcagcttct
     2161 ccgcggcggc gagcatgttg gcggagacgc cacgagcgcg cgcctgggca atcgtctcgc
     2221 ggagcaggta gaagtactcg tagtcctcta tcccctcgcg cagcatctcc cagcggaagg
     2281 agtcgatcgg accggtcagg tgcttccgct tgtcgttcgc tgggtcgcga ttgggtggat
     2341 agatgaagcg gccatccccg ttgccccaat agccgatctg ccccggctgg tagtcatacc
     2401 cggagatgta ggccatcgcg tcctcccacg gattctggac cgccggaggc gggaatgccg
     2461 cgttgctggt ccagtagttg ctcgtccaga cgagaatccc ctcgacgttg tacttcaccg
     2521 tctgccacag ccacatccgc atctcgatgc ccggatgatc gatgaagagc gtgcagtagg
     2581 gcgcctttgg gctggtacag acgtaccacc agatcttctc gcccttggcc tggcgctcgc
     2641 gggaacgctc ctcgtcgtag ttggatgtca ccgggcacca gatatcgaca tagtcggcga
     2701 gctccggctc cacctgttcg gtaagcatcc gcgcgagctt gggcgccgcg cgcttgatga
     2761 gcgccatgcc ctccttgacg aagtcataat cgcgctgttc cggctcgtcg aaccagtaga
     2821 tgtaggcctt atcgagccag cccttctcct ccaagtgatc ctggagttgc cgggcatagc
     2881 tggtgaagag cgcctcatac tccggggttc cctgctcgta ggcaccgatg cgtcccttat
     2941 ggcgggagtg gaaggtgccc ccgcccatgc cttgcaggtg cagccggaac gagttgaacc
     3001 cgagttcgtt gaacgcgcgg tgtgcggccc ggtcaaactc ggtgaaatcg accttcacgc
     3061 ggagcgggtc tgccacttcc tcaaagccgc catccttcag gagctcggtg ccccctgcca
     3121 cgcgcaacga gagggcatcg aaccacgcgg tgccggttgc ctcgcccttc tcgctccaga
     3181 tcactgggcg caggacaatc tgcaccgccc gggagtcttt cggcagccga cctttcagcg
     3241 agacgctctc ctgcttccat gtggtgctcc cccggaagcg caggtcgatg ttccggccag
     3301 acatccatgc accggtggca tcgtagttct gaagtgtgat caggtaggtc tgaccatcct
     3361 tctcggtgcg cgccgcccag gagaggatgt attcggcttc cggatccacg gtcacacgat
     3421 ttcgatagct cgcgccgatg ctcgacctat cggagtcatc gaccaccttc aggcagcgct
     3481 tcccctcaaa gggctgctcc atcacctgct ggccgccatc ccacgtggtc tcgccgaagc
     3541 tcacccggat gggtgccagc gccattgggt cgtagggcga gatccggtgt gccgcgaagt
     3601 tttggaggta gagatcgaag actttcgcga gctcctcctg cgtctccagg ttgtggtagc
     3661 tccgaatagt ggctgtattg agcccgaaag cggtcttgac gtgcgtctcc ttcgggaggg
     3721 tgaagtccca aacgcggagc tggacgggta cttcctgacg ccagggaccg gcggccagct
     3781 ggagagcgcc gcggtagtcg cccgcgggag tgcccgcggg aacgtaaacc gtcacccaca
     3841 gtggctggtt ggtgttcgcg gccacgtcga acgggccctc gaccggcgga agaggatcgg
     3901 gccacagccc aggcgtgccg gcgcggtccg tgggccgctc gacgcgcacg tactcgacga
     3961 ggtggatccg aatgttgctc gcgtcaattc gcgcgccctt cggcccatcc aggtcagtcg
     4021 cggacgctgt cacacctcgc agcgtctggg tcgggcgcag gaccacctgg aatggctcgt
     4081 actcgtgccc cgccgcgctc agccggacta cggactggcg ggtttccggc gccgggcgat
     4141 cacggctcac cttgtgcgtc ggctcggccc accaaacagc cgcggtcgcg tcctgcgaga
     4201 cgaggtagcc gaaatcggcg gcgtagagcg ccgggacatc aaactccgtc ttgcccgcga
     4261 ggaggacctc ccgacccgca gtgacgcgca gcgtggcctg catccgtccg gtctcgcgga
     4321 gcgtgtaggg gagtagcaaa ggcgcttcgc cgccggctgg aacgttcatc ttggtggtga
     4381 actgctgcgg cttgcctctt tccgaggaaa ccgcgaagac ggcggtgacc gggagcgcgg
     4441 cccggcccgg gttcgagacg cgcagccgga cgttgggatc cacgcccggg cgcaacccgc
     4501 ccaggctctc tacggtcacg acggaaggct tgcgctcctg ctcgatctcc aggtaggaag
     4561 tctgcccggc aagatcgcgc ggcgcggctt ccagctcggc ctcgtacgtg tagtcgtgga
     4621 tctggaagct ccccgggtgc gaatcgactc cgctccgctt ctcgccctcc ccgcgcagcc
     4681 gaatctggat ctcatccgcc gggaagagat cggcgggaag ctcggctttg agcgcgccct
     4741 tctccccgag agctccgacc tcgcgccagg ttttcccgtc gcggctcgcg tccacgagga
     4801 gccggccggc cacgtggtag ccgacgttcg cgtggatagt cgccgcgcgc tgcttcgccc
     4861 cgggaagggc gtgccggtag gtgatcaagg cgccatcgct caaaacccag cgattcgtgt
     4921 tgaacccggc ggtgaacccg accagcgggc gcgagtagtt ggagccctcg ccctgcagcg
     4981 gcgcctggaa gatgtagcgc ccctgagaga cgcgctcgcc ctcacccagc tggccgacga
     5041 gcgggagcac gggcgtcacc gtcacatcgt caaaaaggac tttcccggtc gtggaccagg
     5101 tgcccaggcg caggtaggca ccttgaacag agcggggagc catgaagaca cgggtgtaac
     5161 gcacccattc tttcccgggg aggggaaaat cctggttcgc gatgttgaga ccgctgacca
     5221 cgcagcctcc cgtggcgccc tccgaacggg cccagaaggt aagccgatag atgcctcctg
     5281 gctccggcag ccagttatcg catttccaga agtgcgactc tttgccatcg ccgttgatca
     5341 ccaagcaccg ccggcccgtc cgcccgcctt cttcccaggc ggccgtgcta tctgagggcg
     5401 accatgcggt aggccggtct gtgccttcct cgaaagaggc gttcggcacc ggcgccgccg
     5461 aggcagccac caccatcagc gacgcggtga ctacaaggag tgctctcaat atcgccatga
     5521 ccttctcctt ttcggtacac ccacggcttt gcgccagacc tgtgggggtg gatgagcgtg
     5581 atgctgccgg cgcacagatg cagcggatgc agtccgatga tgccaacgga tcaccagtct
     5641 cttccgggga accaggcttc tgctccggat agccggtggg tcccgctgtc ggatgttctc
     5701 cacccgatcc ctgcaccttc ttcgcccgag gggggggcat ctcctcccgc agtctgccac
     5761 gcgagcggcc tgctgggcta tccacagatg acgcagatga atacaggtaa atggggctgg
     5821 cacaggagga agccgcttgc ctgcgaattg acacgctccg ggacggcctg gtacaattca
     5881 gctaagaata atcgaagccg gggacgatgc gcggccaggg atatcgtcgc cacggcctgt
     5941 cgcagtgcaa ggggaacgca tgcacgaagt gggaatcgcg gaggagctgg tgcgccaggt
     6001 ggtggccgcg gcgtgcggga tggcgcgggt gacggaggtg gccgtcgatg tgggcgccct
     6061 ccagtgggtg gactcggacg cgctgcgcca ggcgttcgac gccgcgagcc aggataccat
     6121 cgcggaaggc gcgcgcctgg agctgcgcga agtaccgccg cgcgcggtgt gcggcgcctg
     6181 cgccgcagtg tacgttcccg aggagctggg tatctacgtc tgtcccgcgt gcggggcggc
     6241 tgaaccggag tttgtggaag gcaacgatat cgtcctcgtc agtttggagt gcctttcgcc
     6301 ggaggaagaa aaccgtgagt cgtattgagg tcgtgagcag tgtgctgcga gccaaccagg
     6361 tgcgtgccga ggagacgcgg gcccgcctgg atgccgccgg cgtagcctgt gtgaacctga
     6421 tcagcgcccc gggcagcggg aagacaacgc tgctccagaa gacgatcacc gccctggagg
     6481 ggattccttg cgccgtactg gtgggcgact tgcagacgac acgcgatgcc gagcggttgg
     6541 ccgctgcggg cgcgcccgtc gttcagatca acactgggcg gggttgccac ctcaccgccg
     6601 atcaggtggc cgagggcctg gatcgcctgg atctcagcca gatccaactg ctcttcatcg
     6661 aaaacgtggg gaatatggtc tgcccggccg gcgtcgacct gggcgagcac cggcgcgtcg
     6721 ccctgctcag cgtgccggaa ggcgatgata aggtcgcaaa gtacccgact ctcttccagg
     6781 gcgccgacct ggtgttgctg accaaagtgg atctcctctc cgtgctgccg ttcgcggttc
     6841 gccaagtgga agaggacctg gcccgcgtca acacgcgcgc cccactcgtg cgcctctcga
     6901 cgctcgaagg cacggggatg gatgcctggc tggcgtggct gcgcgcgctc gtggccggct
     6961 gatctattct ctatgatttg ataagaatga tttgataaga gcgggccgcg gggattgggt
     7021 atcgaccctc gcggcccgtc tgcttgtacg ccccctctac ccctatatga cccctctata
     7081 atcctgtccc gcggctgtgc ccctggccgg aagttgccgg cgcggcccgc gttcttctcg
     7141 cgaagaggac tcctgcggac gcgggagaag gatccgggtc aagaggacgc gagcgccggg
     7201 cgctgaagca ctccgaagca cccgggctcg ttgccgcgga aacgggagga agcgatgcca
     7261 ctgctcgcca cgattgcccg cgtggggttc gcgggcctgc tcgcgctggg catggcggca
     7321 cccggccaga ttgaagcggc ggagatccgg ctggaccagg accggctccg gctgcaggtg
     7381 aatgaggcat cggggcggat cactcgcgtc gaagcgagcg gcgcgaacct gacggcaccg
     7441 gatgcagtct cgcgctccgg gtttgccctg gccgatggcc cgaaggcgga gtgcgtgccg
     7501 gtgacgtgcc gggtgacccc gcgcggcgcg ggcgggcttc tctgggatgg ccaggccggc
     7561 gcgctgcgca tcgcgtgcca gtcgctccc
//
LOCUS       DAOSCV010000069         7576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6282_length_7576_cov_3.307539, whole genome shotgun sequence.
ACCESSION   DAOSCV010000069 DAOSCV010000000
VERSION     DAOSCV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6282_length_7576_cov_3.307539"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLY56_02880"
     CDS             complement(<1..409)
                     /locus_tag="PLY56_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase PA-phosphatase"
                     /protein_id="HOJ20453.1"
                     /translation="MCEQPATTKCDRLCGHAEARRDTALAAGVALAISAAASPYVVLP
                     GFAFVLTWHYSPDWHHFLAWAGIAVFFSTGIPFLYILLGTRSGRITDIHVMRREQRRG
                     PFLVSLISSAAGTVLLHLISAPRPLVALGVAIIA"
     gene            840..2744
                     /gene="ftsH"
                     /locus_tag="PLY56_02885"
     CDS             840..2744
                     /gene="ftsH"
                     /locus_tag="PLY56_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ20454.1"
                     /translation="MRALVTILIIGALVFLASGRFGNRMETVEKVPYSKFLAMVREKP
                     ESPILEVTFEKDKIVGEYKRGGKFQTFVPEIFKQTEYLQKELAENNVRFDAQPSHLSD
                     MLQGLLVSVLFPIGILALFWFFILRQAQSGGNQAMAFGRSRAKRFNENVPKVRFEDVA
                     GVDEAKQELAEVVDFLKNAKKFQALGAKIPKGVLLLGPPGCGKTLLARAIAGEAQVPF
                     YHISGSDFVEMFVGVGASRVRDLFETAKTNRPSLIFIDEIDAVGRQRGAGLGGGHDER
                     EQTLNQLLVEMDGFDANSGVILIAATNRPDVLDPALLRPGRFDRRVVVDHPDAHGRRA
                     ILEVHVKGKPLAEDVDLDALSRRTPGFSGADLANLVNEAALIAARREKTQIWLEDFED
                     AIDKVVAGPERRSRIMSGRVKEMVAYHEVGHAIVGHLMPHVDPVHKVSILPRGMALGY
                     TMTLPVEDKYLTSKSELLDQITFALGGRAAEELVFDEVTTGAENDLNRATDTARRMVC
                     EFGMSEKLGPLTLGRRHGNPFLGRDLMEDRNYSEEVASAIDQEVRRIIDECYDRARNI
                     LIANRARMRHVVEVLLEKETIETDEFIRLMEEPVPEPAGKVTDHEEDATVVADDTAVP
                     AGAAGASRQD"
     gene            2975..4153
                     /locus_tag="PLY56_02890"
     CDS             2975..4153
                     /locus_tag="PLY56_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ20455.1"
                     /translation="MSATISPPPAEREPVMSRDLAVLSASFFLIFMGAGAFQQYLIRF
                     MKDVTGRTVTESSWVLATIYLSFLVWRVLAAYTIRWLGDQRSIFLGQVTYTLFLVFAI
                     FTREFWVLLAAAALWGWGASAMWIASSTQVLDASARTRYGSASGVFYAATHLGQWLGV
                     ILLGWLFTRGGWSPLLTVALVVSVLGNAVALAVPRKYVPREEPRISKLFGVLRSPESK
                     ALAVLLFVSSAGFGLILSLFGTLLSGQTLVWVTSGFYGGRLVSSWYAGSISDRLGRRG
                     VLVTGFALAAAGMACALQAGSLAALFVAALTLGVQSGTVPVAAMAMVGDFTEPSRRHM
                     AFGAIYVWRDLGVALTILGAQQLVSRVGQGVCFATFAVLFAVCALLSLGLRERKGSAG
                     "
     gene            4462..5085
                     /locus_tag="PLY56_02895"
     CDS             4462..5085
                     /locus_tag="PLY56_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20456.1"
                     /translation="MCSKRRDGFTLIELLVVIAIIAILAAILFPVFARARENARKSTC
                     QSNLKQLGNANMMYAQDYDERTLPYSSLPSGGGERWFILVQPYLKNSDVLKCPSQRIT
                     TGQPTSYGLNRATGSLGEGGTPGVSLATIQNPSETLAFLDTRAHDNEAEGFATFYAPP
                     AGNPTWGTYGCLSDRHSDGLNCAFYDGHVKWFKRDALWAKPEWFDFN"
     gene            complement(5239..6261)
                     /locus_tag="PLY56_02900"
     CDS             complement(5239..6261)
                     /locus_tag="PLY56_02900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20457.1"
                     /translation="MTYRCAFLGCGPRARSHALAYQQITRGEIVALCDLNESRLNDFG
                     DTFSVAARYTDLDEMLRKEKPDVVHLVTPPTLRVGLMTRLAEAGVPGVIVEKPICIGA
                     DDYKALRELEARSETRFVVNHQLRHHPRILEFLGEVSAGKIGQVRFLDATARLPMAGQ
                     GVHVLDLMFAFNGYAKPTSVFGASSGYDDINGTHPTAKSAEYLITFENGARGVLMAGV
                     GAPTFTEGPTYMHKRIAVYGTHGFRHWRMDAWERSLPDGTVESQTHVYREEDIIGQAG
                     LTNALFDWLDDPARVHPNNLATSLDEWLVILAGYASTVEARAIELPFDPPADLLDQFK
                     RFVGAA"
     gene            complement(6261..7067)
                     /locus_tag="PLY56_02905"
     CDS             complement(6261..7067)
                     /locus_tag="PLY56_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20458.1"
                     /translation="MNFKDAGWVWEGQGLDPGVFPSVFGVGEGARYFGVSRAWYMFHP
                     NDDLGMEKLRGLKAVICDIAKWKYRDGEDGGITHWVDAAPEAVRAEAETISQLSTRYS
                     NIAGAVHDDMLGLIRREGYEPSDYAPIYQALKSKNPKLNLWAVVYSHELDSEAWAPYL
                     PFIDVVSLWVWEAQNLPRLEEYVARCRTIFPGKQINVGSYLRNYPTLSPVPMELLRVQ
                     WETMLDLFRKGVIDSYSILGTVLIDGHRQQAEWVRDFIATNANVPKGKVK"
     gene            complement(7484..7561)
                     /locus_tag="PLY56_02910"
     tRNA            complement(7484..7561)
                     /locus_tag="PLY56_02910"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7524..7526),aa:Pro,seq:cgg)
BASE COUNT         1325 a         2270 c         2543 g         1438 t
ORIGIN      
        1 tagcgatgat ggcgacgccg agagcgacga gtggacgcgg cgcgcttatg agatgaagaa
       61 gcacggtgcc ggccgcgctg ctaatcaggg aaacgaggaa gggaccgcgg cgctgttctc
      121 gacgcatcac gtgaatatcg gtgatccgtc ccgagcgcgt gcccagcaag atgtagagga
      181 atggaattcc ggtggaaaag aaaacggcga tgccagccca ggcgaggaag tggtgccaat
      241 ccggactgta atgccacgtc aacacgaacg cgaagccagg caggaccaca taggggctgg
      301 cggccgccga gatcgccagc gctaccccgg ccgcgagtgc ggtatcgcgg cgcgcctcgg
      361 catgcccgca gaggcggtca cacttcgtcg ttgctggctg ctcgcacacc aggggcttcc
      421 tttccgtctc cgactctctt cgccatctat cacccggatc ctgcgtcgtg tcatttgtca
      481 ccccccttcg aattgtgcta taatgacgtc agtagtctga gggcaccgca ggccgtgaat
      541 gcaagacgtc agtcgtttcc gcaacaggcc ggctgccctt ttttcgtaag gtgcgagaca
      601 ccgcagccgt tgatcgaagc gggggctcac cgggctgcat cggtcctccg ggaagcagtt
      661 gggggcccgt tccgagaggc cttcgcgttc gccggccgtg gggtggcgcg gtgcggcgtt
      721 gcgcgattcc cgtggcggcg ggcgaatgac gttggcggtg ctgcgttgga agttgcggta
      781 ggtgaagacg cggcgccaat atgtttgttc ggaggagcgt tttgaacata ggtagatctg
      841 tgagagcgct agttaccata cttatcattg gtgcgctcgt gttcctggca agtggccggt
      901 tcggcaatcg gatggagacc gtggagaagg tcccctattc gaagttcctt gccatggtgc
      961 gcgagaagcc agagtctccg attcttgagg tgacgttcga aaaggacaag atcgtcggag
     1021 agtacaagag gggcgggaag ttccaaacct tcgtgccgga gatcttcaag caaacggagt
     1081 atctgcaaaa ggagctcgcg gagaacaacg tccggtttga cgcgcagccg tcgcatcttt
     1141 cggacatgct tcaggggctg ctcgtctcgg tgcttttccc gattgggatc ctggcgctgt
     1201 tctggttctt catcttgcgc caggcgcaga gcggtggcaa ccaggccatg gccttcgggc
     1261 gtagccgcgc caagcgcttc aacgagaacg tgccgaaggt gcgtttcgag gatgtggccg
     1321 gcgtggatga ggccaagcag gagctggccg aggtagtcga cttcctgaag aacgccaaga
     1381 agttccaggc gctgggcgct aagattccca agggcgtgct gctgctggga ccgcctgggt
     1441 gtggtaagac gttgctcgcg cgtgccatcg caggcgaggc gcaggtgccg ttctaccaca
     1501 tcagtggttc ggacttcgtg gagatgttcg tcggcgtggg cgcttcccgc gttcgtgacc
     1561 tcttcgagac ggccaagacc aaccggccta gcctcatctt catcgatgag attgatgccg
     1621 tcggccgcca gcgcggcgcc ggtcttggcg gtggccatga tgagcgggag cagactctga
     1681 accagctcct ggtggagatg gacggcttcg atgccaactc gggcgtcatc ctgattgcgg
     1741 ccacgaaccg cccggatgtg cttgatccgg cgctgttgcg ccccggacgc ttcgaccggc
     1801 gcgtggtcgt ggatcacccc gatgcccatg ggcgtcgcgc catcctggag gttcacgtca
     1861 agggcaagcc gctcgccgag gatgtcgatc tggacgcgct gagccggcgc acccctgggt
     1921 tctccggcgc cgatctggcg aacctcgtga acgaggcggc gctcatcgcc gcgcgacgcg
     1981 agaagacgca gatctggctg gaggatttcg aggacgccat cgataaggtg gtcgccggtc
     2041 cggaacgccg cagccgaatc atgagcggca gggtgaagga gatggtcgcc taccacgagg
     2101 tggggcacgc cattgtgggt cacctcatgc cccatgtgga cccggtccac aaggtgtcga
     2161 tcctgccgcg tggcatggcg ctgggctaca cgatgacgct gccggtagag gataagtacc
     2221 tcaccagcaa gagcgagttg ttggaccaga tcaccttcgc ccttggcggg cgtgccgccg
     2281 aggagctggt gttcgacgag gtgaccacgg gcgcagagaa cgacctgaac cgggccacgg
     2341 atacggcgcg gcgaatggtg tgcgagttcg gtatgagcga gaagctcggc ccgctcaccc
     2401 tgggccggcg ccacggcaat ccgttcctcg ggcgcgacct catggaggat cgcaactatt
     2461 ccgaggaggt ggccagcgcc atcgaccagg aagtgcgccg gatcatcgac gaatgctacg
     2521 accgtgcgcg caacatcctg atagccaatc gcgcgcggat gcgacacgtc gtggaagtcc
     2581 tcctcgagaa ggagaccatc gagacggacg agtttatccg cctgatggag gagcccgtgc
     2641 cggaacctgc tggtaaagta accgaccacg aggaggatgc caccgtcgta gcagacgaca
     2701 cggcggtacc tgccggcgcg gcaggcgcct cgcgccagga ctaagactct cgcgccacgg
     2761 cgcgaagggt aaccggagcc ggtccgggag cagatgctct cccggaccgg ctttttttat
     2821 gggcgagccg gcgagcgccc cccgcgcggg cgcaatcggt cgcgtggagg tatcgttatc
     2881 cgcagatcga cacagatgaa cacggataac acgaacatat attcgcgcca gattggcggt
     2941 tgacaaagga gcgcgggcgt gctaaggtga gcgcatgagt gcaacaatct ctcccccgcc
     3001 ggccgagcgc gagccggtca tgtcgcgcga tctggcggtg ctctcggcat cgttcttcct
     3061 cattttcatg ggtgcgggcg cattccagca gtacctcatt cgcttcatga aggatgtcac
     3121 cgggcgcacc gtgaccgagt cttcctgggt actggccacg atctaccttt ccttcctggt
     3181 gtggcgtgtt ctggcggcct ataccattcg ctggctgggc gaccagcgtt ccatcttcct
     3241 cgggcaggtc acctacacgc ttttcctggt gtttgccatc ttcacacggg agttttgggt
     3301 gctgctggcg gcggcggcgc tttggggctg gggtgcctcg gcgatgtgga ttgccagtag
     3361 cacccaggtt ctggatgcct cggcgcgcac gcgctacggc agcgcctcgg gagtcttcta
     3421 cgcggcgacc cacctggggc agtggctggg cgtgatcctt cttgggtggc tcttcacccg
     3481 cgggggatgg agcccgctgt tgaccgtagc gctggtggtg agcgtgctcg ggaacgcggt
     3541 ggccctggcg gtgccccgca agtacgtgcc tcgcgaagag ccgcgcatca gcaagctctt
     3601 tggtgtcctc cgctcgccag agagcaaggc gctggccgtc ctcctctttg tctccagcgc
     3661 cgggttcggg ctgattctca gcctctttgg caccctactc agcggccaga ccctggtgtg
     3721 ggtcacctcc gggttctatg gagggcgtct cgtctccagc tggtacgcgg gaagcatctc
     3781 cgaccgtttg gggcggcgcg gggtgctcgt cacaggtttc gcgctggcag cggccggcat
     3841 ggcgtgcgcc cttcaggcgg gcagtctggc cgcgctgttc gttgccgcgc tcacgcttgg
     3901 ggtgcaatcg ggcacggtgc cggtcgccgc gatggcgatg gtcggcgatt ttacagagcc
     3961 gagccgccgc cacatggcct tcggcgcgat ttacgtgtgg cgcgatctgg gcgtggcgct
     4021 caccatcctt ggggcgcagc aactggtgag ccgggttggc cagggagtct gctttgccac
     4081 cttcgcagtg ctctttgctg tgtgcgcgct cctctccctt ggtctacgcg agaggaaagg
     4141 aagcgcgggg tgagaacgaa ggattccgcg cttggttggg gaatctcttt ctgggcgtgg
     4201 ccgctgtacg ggcccttgcc atccagctgg tggagcctgg tagccggtga agagcaccga
     4261 tgagggagat gagctctttg atggaggtgg aaggtctcct gtcaggtagt ctcgagcacc
     4321 agccgcgtgc ctgtcgggtg ctggcgcgga tgggaaggcg cccctaccga gaggacgcgg
     4381 cgtaaggggc ctggcagacg gcttacctgc tgtacccgta cggcccgtat actatcgcga
     4441 tcacgaggag ggagtctcga aatgtgcagt aagagacgcg acggattcac gctgatagaa
     4501 ttgctcgttg tcatagccat tattgctatc ctcgcggcaa ttctcttccc ggtcttcgcg
     4561 cgcgcgcggg agaacgcgcg caagtccacc tgccagtcca acctgaagca gctgggaaac
     4621 gcgaacatga tgtacgccca ggactatgac gagaggacat tgccctactc atcactcccg
     4681 tccggcgggg gcgagaggtg gttcatcctg gtgcagccct acctgaagaa cagcgacgtc
     4741 ctgaagtgcc cgtcgcaacg gatcaccacc gggcagccga ccagctatgg cctgaaccgc
     4801 gcgaccggct ctctcggaga aggcggtact ccgggggtgt ccctggccac catccagaac
     4861 ccctccgaga cgctcgcttt cctggatacg cgcgcgcatg acaatgaggc cgaggggttc
     4921 gccacgttct atgccccgcc ggcggggaat ccaacctggg gcacgtacgg gtgcctctcc
     4981 gaccgccact cggacggctt gaactgcgcg ttctacgatg gccacgtgaa gtggttcaag
     5041 cgcgacgccc tctgggcgaa gcctgagtgg ttcgatttca actgatgccg tcgatcgggg
     5101 gcgagcgcgg ctctcggcgg tgaaggcgcc agggcggtgg aacccctgcg gtctggcttt
     5161 ccgcggatag gaagaggcga gggtccatga tgggccctcg cctggcattt ggttgcgcgc
     5221 cgttgccggc gaacgaggct atgcggcgcc cacaaagcgc ttgaactgat cgaggaggtc
     5281 ggccggcgga tcgaagggca gttcgatggc gcgcgcttcg accgtgctcg cgtaacctgc
     5341 caggatcacc agccactcgt cgagggatgt ggccaggttg ttcgggtgaa cgcgcgccgg
     5401 gtcgtccagc caatcgaaga gggcgttcgt gagccccgcc tgcccgatga tgtcctcctc
     5461 acggtagaca tgcgtctggc tctccaccgt gccatccggg agcgaccgct cccaggcatc
     5521 catgcgccaa tggcggaagc cgtgggtgcc atagacggcg atccgcttgt gcatgtacgt
     5581 ggggccctcc gtgaaggtag gcgcgcccac gcctgccata agcacccccc gcgcgccatt
     5641 ctcgaaggtg atcaggtatt cggcggattt cgccgtggga tgggtgccgt tgatgtcgtc
     5701 gtagccggaa gaggccccga agacgctcgt gggcttcgca tagccgttga aggcgaacat
     5761 caggtcgagc acatgcacgc cctgtccggc catcgggagc ctcgcggtgg catcgaggaa
     5821 gcgcacctgc ccgatcttcc ctgcggatac ctctcccaga aactccagga tgcgcgggtg
     5881 gtggcgaagc tggtgattca ccacgaagcg agtctcgctg cgcgcttcta gctctcttag
     5941 cgccttgtag tcgtcggcac cgatgcagat cggcttctcg acgatcactc ctggcacgcc
     6001 cgcctcggcg agacgggtca tcagtcccac gcgcagcgtc ggcggggtga ccagatggac
     6061 gacatccggc ttctccttgc gcagcatctc atccaggtcg gtgtagcgcg cagcaacgga
     6121 gaaggtatcg ccgaagtcgt tgagccggga ttcattcaga tcacacaggg caacgatctc
     6181 gccccgggtg atctgctggt aggccagggc atggctgcgg gcgcggggcc cgcatccgag
     6241 aaacgcgcag cggtaggtca tcacttcacc ttgcctttcg gcacgttggc gttggtagcg
     6301 ataaaatcgc ggacccactc ggcctgctgc cggtggccgt cgatcagcac ggtgccgagg
     6361 atggagtagc tgtcgatcac tcccttgcgg aagaggtcga gcatcgtttc ccactggacc
     6421 cggagcaact ccatcggcac gggagagagc gtcgggtagt tgcgaagata cgacccgacg
     6481 ttgatctgct tccctgggaa gatggtgcga caacgggcga catactcctc caggcggggc
     6541 aggttctgcg cttcccaaac ccagaggctg actacatcga tgaaggggag atagggcgcc
     6601 cacgcctcgc tatccagctc gtggctgtag actacggccc acaggttgag cttggggttc
     6661 ttgctcttga gtgcctgata gatgggagcg taatccgagg gctcgtagcc ttcgcgtcgg
     6721 atcagcccga gcatgtcatc atgcacggca ccggcgatgt tagaatagcg cgtggagagc
     6781 tggctgatcg tctcggcctc ggcgcgcacc gcctcagggg cggcatccac ccagtgggtg
     6841 atcccgccat cttccccgtc gcgatacttc cacttggcaa tgtcgcagat gacggctttc
     6901 aggccgcgca gcttctccat gcccaggtca tcgttgggat ggaacatgta ccatgcgcgc
     6961 gatactccga agtagcgggc gccttcgccc actccgaaga ccgagggaaa gacgccagga
     7021 tcgaggccct ggccctccca gacccagccg gcatccttaa agttcatgtg tctcctcctg
     7081 atatatcgac cggcctcgtt gccgcgcacc gcactgccat tccccattgc cggcgtgtga
     7141 tcgagggcct gttggcgccc atcgttgggc tacatctgtt gagcagggtg gctagttccg
     7201 ccagcgcctg cccatccggt agagttcggc ggagacacgc ggcatccttg cggctgaagc
     7261 gcaagcgggt gatctgccag gaaagcgccg cgaccacgag gaaataaccc ttagggaaga
     7321 gcgtatccat gcgcaatgga gcgcctgccc agggatctgc cgctagcacc ggcgagctgc
     7381 tcttcggttg cgcgatctgg agagagaaac cggttgggtg gggtggggga ctgacacctt
     7441 cttcctggag cttgagcagt gggattgtca gggaaggaaa gagtggtcgg ggcgagagga
     7501 tttgaacctc cgacctcacg gtcccgaacc gtgcgctctg accaaactga gctacgcccc
     7561 gtccgacatg gcagat
//
LOCUS       DAOSCV010000070         7500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6364_length_7500_cov_3.215850, whole genome shotgun sequence.
ACCESSION   DAOSCV010000070 DAOSCV010000000
VERSION     DAOSCV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6364_length_7500_cov_3.215850"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..>810)
                     /locus_tag="PLY56_02915"
     CDS             complement(50..>810)
                     /locus_tag="PLY56_02915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ20459.1"
                     /translation="AAGAGSRLRSVSSVPISARKVAITFDDGPHPLYTPPLLDLLERY
                     QARCTMFLVGSQAESHPEIVREIAEKGHEVANHTYSHPNLRTLDPRGIERELLRGRSV
                     LQRISGKKIRLFRPPGGNADRTVEEIAAGYGLTTVYWDLFDSWLQRYDEATVLQKLLG
                     GIKPGSIVLLHNGSNKTAPILEPFFEELTRQGYQLVTVSELLAEAAPGIQPPTEVEEA
                     PELRDEEPAAPAAPETAPAHARADARPEGRAAAP"
     assembly_gap    811..820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<821..2023)
                     /locus_tag="PLY56_02920"
     CDS             complement(<821..2023)
                     /locus_tag="PLY56_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20460.1"
                     /translation="MSRDRSFPTVGGLLRVAGIVLAASVTLLSPVAAEGGYLATVQDA
                     TRLLSQGQIAEARRAFQRAREADMNDALAAVGLGYALDVEGKAAEARGELELALKLDG
                     QGRACRWAHALVALNNARLDEAIQHLEAIRKEGAPTPELWVALAYARCASGDYAGARE
                     ALDAAGRPDQLGTATRSMASLVRGAVAYATGDYARAVEVLRDAARHLPANTYFDNVPS
                     RRTPLLPEARTSRREALALVSASSLGPAVRTRSGVVRLHLDPFRFPGASYAFFLVDGK
                     TVHGTNAPPFRYDWDTRRVRNGYHKVVVRAEDSMGEVLGQTEEVFLIHNEGVTFPPAY
                     PEEEYTRADEALSRAMILQPDVMATRYLLGLALKHMGRVDEAAAELEQVMAHDPDYGR
                     ARQELRSIY"
     gene            complement(2020..3657)
                     /locus_tag="PLY56_02925"
     CDS             complement(2020..3657)
                     /locus_tag="PLY56_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20461.1"
                     /translation="MLLRDHEQCAADAAGRLPQAWRPANSSPRRSGLRRVLWAAPWWV
                     LLLFAIVTGASAQNAPESEELYRIRVTNAVGGAVEVSGDGGQTYTPVGKVVTPATRLI
                     QGFAASRWAPVGSVAATAIHGLRLKVRNSTPKDPKGVANCMMMSLVPREFHTIPKGYG
                     GHRPGNSGIYTDIPAGQGIFRNLAPLVASPVRLESEGRLVPFPADYQPRVGDRIVILV
                     YLPPMLPREIVFENWKGGLVTAHFEGRDPHPVARVARPVLGIGRYDGTAYTGVGRINT
                     NHCGVITVSTAPVSGSLLPEGQGSERRGGFQIEPADHARDVSYNAPQVMVVEPLEGQG
                     ALEGQPPLFAGYIGLQWDPRSVAHSLGVEVKIDNGPWEPMPELVGVMNDAFAASALMR
                     HFRKLGVARRITSGVTHLRLVFPRLDRAAVARYLAEYATPGPAETTLGEEEMAGPAGP
                     AGPATPVHGVITLNASFRNPEDLQRIAVVTVYVDGRIVAIMNVAPFRHQLDTRRLPNG
                     RHEVELRALDGQGGIVAQKKMSIMVENAASEGGAPAS"
     gene            complement(3661..5436)
                     /locus_tag="PLY56_02930"
     CDS             complement(3661..5436)
                     /locus_tag="PLY56_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ20462.1"
                     /translation="MRVPLFARESVGLVGKRVIWALGVIGACLGMAGPLAAQSAGAPA
                     QSVSAPLLSRPLTQHAGLEGRLIWVDGYANLDWLSSRAGIASVVEKMRQAHLNTVVFG
                     AKMFGGQALYPSAIAPPLTEWKGHQVPPGHDMLGTLITEAHARGLKVHAAVDVFADGH
                     RLVGIGPAYEHPEWQTVVYTVRRELVTADGQRLPIYAVNTAVSGEVVTAYTPGYGWRK
                     PRGDSPDETRVLAVLAGPAEEMRIISVTDCDLMRDGQPVEAPSDGLLLYGRGQMGDWM
                     LRNLRVGERVRFTTVDKFVPIGSDEQEGSAIFLNPVHPEVRARALRILQEIVRNYDVD
                     GISLDRMRFSGFNADFSDYTRARFEDAIGRKVERWPDDIFKIDPIPGRPIVRGPLYGQ
                     WIEWRAAQITSFVKEAVAAVRAIKPEIRIGAYVGSWYHEYWREGVNWASNDYFGGYDW
                     MTATYGQTGYAQEVDYITTGCYYPRSTAEAALLEDAPPERTVEMAAQLSSQVVNDAAF
                     VYAGLYLIYYKQNPEALKEAIAAARQHSQGVMLFDTVYVNQYDYWNVIGDVFAAPSTA
                     PHDVPGLLDSVRQVRKTVRAAIPGR"
     gene            complement(5443..7341)
                     /locus_tag="PLY56_02935"
     CDS             complement(5443..7341)
                     /locus_tag="PLY56_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20463.1"
                     /translation="MARARGALSGCARAGAGAWGYAYLFLLFALLYRLTASRDYTFDS
                     LSYALQIERFTRTGEYSWLFHQHHLLFNALGLGAFRAFRAAGLQVGTLDALQGMNALL
                     GAAGVVLFAWIAGHVCRHRGAGLLAGAGLGLSYGYWTCSTDGRVNIAGVVVLAGVLGA
                     MAVLLGARSMESRASYLRWAGVLGGLHLLAVLLHQSHALVAPAAVLALWLSPGRPRWQ
                     GIGVYLATTLGLGTLAYTVASVLANGSSEPSRVLAWAMTYAHRGHWWSLDLGRNLLLD
                     ARALRHVFVAQPALAGGDAPAGGDLLFLLAGSLAAGGAVVAVAWGAIRYLARVGESDP
                     RGRGIRRSRYWFTPRRPASFDAPVGSLWTRAGYRIGRWAHAALRDFEAMTGIPGPAQA
                     GALMASAGGLRRLYAVLGVWGFAYAAFFTFWNPGYFAFWVPILIPAWLLAALFLASTP
                     GRRLWRWAGAMALLLLLATNNAIVGIGPHARPENNGRLQKALAVRESTRPGDVIVVAG
                     TGDYADAEVYLPYFARREVLAVNTYLTGAPSLSVGLEQLRVEMDRTLEAGRRLFIHEE
                     LLSSEGPYAALRARHGMTRARFIAFLHRHYRLERPVRWRRAGARASGSAAGSGSTTGV
                     TAVCAIRR"
BASE COUNT         1337 a         2623 c         2423 g         1107 t
ORIGIN      
        1 atgcggcggg tgtcatcccg gtgctaccgg cactcgactt catcagtggc tagggcgccg
       61 cagcgcgccc ttcgggccgc gcatctgccc gcgcgtgagc cggcgccgtc tcgggggcgg
      121 cgggcgccgc cggctcctcg tcgcgcaact caggcgcttc ctcaacttcc gttggcggct
      181 ggatccctgg cgcggcctcg gcgagcaact cggagaccgt gaccaactga tagccctgcc
      241 gcgtcagctc ctcgaagaac ggttccagga tcggtgccgt cttgttgctc ccgttgtgca
      301 ggaggacgat agagcccggc ttgatcccac cgaggagctt ctgcaacacc gtggcctcgt
      361 cgtacctttg cagccacgag tcgaagagat cccaatagac ggtggtgagg ccgtagccgg
      421 cggcgatctc ctcaacggtc cgatcggcgt ttccgccggg cgggcggaac agccggatct
      481 tcttgccgga gatgcgctgg agcacagagc ggccacgaag cagctccctc tcgatgccgc
      541 gcgggtcgag cgtccgcagg ttcgggtgcg agtaagtgtg gttggccacc tcgtgcccct
      601 tctccgcgat ctcgcgcacg atctccggat gcgactcggc ctgcgagcca accaggaaca
      661 tcgtgcatcg cgcctggtag cgctccagga ggtccagaag cggcggcgtg tagagcggat
      721 gcggcccgtc atcgaaggtg atggcgacct tgcgcgcgga gatgggcacg gagctgacac
      781 tgcgcaagcg ggagcctgct cctgccgcgt nnnnnnnnnn gtagatgctt cgcagctcct
      841 ggcgcgcccg accatagtcc ggatcgtgcg ccatcacttg ctccagctcg gccgcggcct
      901 cgtcgactcg gcccatgtgc ttcagggcca gccccagcag gtagcgcgtc gccatcacat
      961 ctggctgtag gatcatggcg cgcgagagcg cctcgtcggc gcgcgtgtac tcctcctccg
     1021 gataggcagg tgggaaggta accccctcgt tgtggatgag gaacacctct tcagtctggc
     1081 ccagcacctc tcccatggag tcctcggcgc gcaccaccac cttatggtag ccgttgcgga
     1141 cgcgacgcgt gtcccaatca taccggaacg gcggggcatt ggtgccatgc acggtcttcc
     1201 catccaccag gaagaaggca tagctggcgc ccgggaagcg gaagggatcc aggtgcaggc
     1261 ggactacccc cgatcgtgtg cgtaccgccg gaccaagaga ggacgcagag acaagagcga
     1321 gggcttcccg acggctcgtc cgtgcctctg gaagaagcgg ggtgcgccgg gagggcacgt
     1381 tatcgaaata ggtgtttgcc gggaggtggc gcgccgcgtc gcgcaggact tccaccgcgc
     1441 gcgcgtagtc gcccgtcgcg taggccacgg cgccccgcac caggctggcc atcgagcgtg
     1501 tggcggtgcc aagttgatcc ggcctgcccg cggcatccag cgcctcgcgt gctccggcgt
     1561 aatcccccga ggcgcagcga gcgtaggcga gtgccaccca aagctctggc gtgggagcgc
     1621 cctcctttcg gatcgcttcc agatgctgga tggcctcatc aagacgcgcg ttgttcaacg
     1681 ccacgagcgc gtgcgcccag cggcaggcgc ggccctggcc atccagcttc agagcgagct
     1741 ccaactcgcc gcgtgcttcc gctgccttcc cctccacatc gagggcatag ccgagcccca
     1801 cagcggcgag ggcatcgttc atatccgcct cgcgagcccg ctggaacgcg cgccgggcct
     1861 ccgcgatctg cccctgggac agcaacctcg tcgcatcctg aacagtggcc aggtagccgc
     1921 cctccgccgc gacgggcgac agcagagtga cagacgcggc gagcacgatg cctgccacgc
     1981 ggagaagccc cccaacggta ggaaaagagc ggtcacggct cacgacgcgg gagctcctcc
     2041 ctcggatgca gcattctcca ccatgatact catcttcttc tgggcgacaa tgccgccctg
     2101 tccatccaac gcgcgcagct ctacctcgtg ccggccattg gggaggcgac gcgtgtccag
     2161 ctgatgccga aacggcgcca cgttcatgat cgccacgatc cggccatcga cgtacaccgt
     2221 cacaacggcg atacgctgca gatcctccgg gttgcggaac gaggcgttga gtgtgatcac
     2281 cccatgcacg ggggtggccg gccctgccgg ccctgccggc cccgccatct cctcttcgcc
     2341 gagcgttgtc tctgccggcc caggggtagc atactccgcg agataccggg caacagcggc
     2401 tcgatccagc cgggggaaaa cgaggcggag atgggtcacc cccgaggtga tgcggcgtgc
     2461 cacgcccagc ttcctgaaat ggcgcatcaa cgccgaagcc gcgaaggcat cgttcatcac
     2521 ccccacgagt tcgggcatcg gctcccaggg cccgttatca atcttcactt ccacgccgag
     2581 cgagtgcgcc acagagcgcg ggtcccactg caacccgatg tagccggcaa agagcggcgg
     2641 ctgtccttcc agggcgccct gcccctccag gggctctacc accatgacct gtggcgcgtt
     2701 gtaggagaca tcgcgcgcgt ggtctgccgg ctcgatctga aagccccctc gcctctcgga
     2761 tccctgccct tccgggagca gcgagccgct caccggagcc gtcgagacgg tgatcacccc
     2821 gcagtggttg gtgttgatcc ggcccacccc ggtataggcc gtgccatcat accggccgat
     2881 acccagcacg ggccgcgcca cgcgggcgac ggggtgtggg tctcgcccct cgaagtgggc
     2941 cgtcaccagg ccgcccttcc agttctcgaa gacgatctcg cgggggagca tcggcggcag
     3001 atagacgagg atgacgatgc ggtcgcccac gcgcggctgg tagtcggccg ggaagggaac
     3061 cagccggcct tcactctcca ggcgaacggg gctggccacg agcggcgcca gattgcggaa
     3121 gatcccctgg ccagcgggaa tgtcggtgta gatcccggag ttgccggggc gatggccccc
     3181 gtagcccttg gggatggtgt ggaactcccg cggcaccagg ctcatcatca tgcagttggc
     3241 gacgcccttg gggtccttag gcgtcgagtt gcgcaccttc aggcgcaacc cgtggatcgc
     3301 ggtagcggcc acgctcccca cgggcgccca ccggctcgcc gcgaagccct ggatgagtcg
     3361 ggtcgccggg gtcaccacct tgcccacggg cgtgtaggtt tgcccgccgt cgccactcac
     3421 ctcgacggcg ccgcccacgg cgttggtcac ccggatccgg tagagctcct cggactcagg
     3481 cgcgttctga gcactggcgc cagtcactat ggcgaagagg agcagcaccc accagggggc
     3541 ggcccaaagg acgcgacgca agccagaccg gcgcggcgac gaattggcgg gccgccaggc
     3601 ctgcgggagg cggccagcgg catctgcggc gcactgttca tgatcacgta agagcatcag
     3661 ttatctgcct gggatcgcgg cacgaacggt tttccgcacc tgtcggacgg aatccaacag
     3721 accgggcaca tcatggggcg cggtcgaggg cgcggcgaat acatccccaa tgacgttcca
     3781 atagtcgtac tggttcacat acacggtatc gaacagcatc acgccctgcg agtgctgccg
     3841 tgccgcggcg atcgcttcct tcagggcctc cgggttctgc ttgtagtaga tgaggtagag
     3901 gccggcatag acaaacgccg cgtcgttcac gacctggctg gagagctgcg cggccatctc
     3961 gaccgtgcgc tcaggaggcg cgtcctccaa cagcgccgcc tcggcggtgc tgcgcgggta
     4021 gtagcacccg gtggtgatgt aatccacctc ctgggcatac cctgtctgtc cataggtagc
     4081 cgtcatccag tcgtagccac cgaagtaatc gttgctggcc cagttgacgc cctcgcgcca
     4141 atactcatgg taccaagacc caacgtaggc gccgattcgg atctccggct tgatcgcgcg
     4201 caccgcggcc actgcttcct tcacgaagga ggtgatctgc gcggcacgcc actcgatcca
     4261 ctggccgtag agcggcccgc gcacgatggg ccgccccggg atcgggtcga tcttgaaaat
     4321 gtcgtccggc cagcgttcca ccttccggcc gatcgcatcc tcaaaccgag cccgcgtgta
     4381 gtcgctgaaa tcggcgttga acccgctgaa gcgcatccga tccaagctga tcccatccac
     4441 gtcatagttg cgcacgatct cctgcaggat gcgcagggca cgcgcccgta cctcgggatg
     4501 caccgggttg aggaagatcg ccgagccctc ctgctcgtcg ctgccgatcg ggacgaactt
     4561 atccaccgtg gtgaagcgca cgcgctctcc cacacgcagg ttgcggagca tccagtcgcc
     4621 catctggccg cgcccgtaga ggagcagccc atccgaaggc gcctcgaccg gctggccatc
     4681 gcgcatcaga tcgcagtccg tgacggagat gatccgcatc tcctccgccg gcccggccag
     4741 tacagccaac actcgcgtct catccgggct gtcgccgcgc ggcttgcgcc agccgtagcc
     4801 gggcgtgtag gcggtcacga cctcgccact cacggcggta ttgaccgcgt agatcggcag
     4861 gcgctggcca tcggcagtca ccagctcgcg ccggaccgta tagacgaccg tctgccactc
     4921 aggatgctcg taggccggcc cgatacccac cagacgatgg ccatccgcaa agacatcgac
     4981 ggcggcatga accttcaggc cgcgcgcgtg cgcttcggtg atgagagtgc cgagcatatc
     5041 gtggccaggc ggaacctgat ggcccttcca ctcggtgagg ggcggggcaa tcgcggaggg
     5101 atagagcgcc tgcccgccaa acatcttcgc gccaaagacg accgtgttca ggtgcgcctg
     5161 ccgcatcttc tcgacgacgc tggcaatccc cgcccgcgac gagagccaat cgaggttcgc
     5221 gtagccatct acccagatca gccggccttc caggcctgcg tgctgcgtga gcgggcgcga
     5281 aagcaggggc gcggagacac tctgagcagg cgcaccagcc gattgagccg ccaggggtcc
     5341 ggccatgccg aggcacgccc cgatcacccc cagcgcccag atgacgcgct tccccaccag
     5401 acccacactc tctcgcgcaa acagaggcac ccgcatctca cctcatctcc ggatagcgca
     5461 caccgcagta acacctgtgg tagacccaga acccgccgcg gacccagaag cccgagcgcc
     5521 cgcccgccgc cagcgaacgg gacgctccag gcggtaatgg cgatgcagaa aagcgatgaa
     5581 ccgcgcccgc gtcattccat ggcgggcgcg aagcgctgcg tagggccctt ctgaactcaa
     5641 tagttcctcg tgaatgaaga gcctcctccc cgcttccagc gtccggtcca tctccacgcg
     5701 cagttgctcc aggcccacgc tcagcgaagg cgcacctgtc aggtaggtgt tcaccgccag
     5761 gacctccctg cgcgcgaaat agggaagata gacctcggcg tcggcataat cgcccgtgcc
     5821 cgccacaacg atcacatcgc ccgggcgcgt gctctcgcgc accgcgagcg ccttctgaag
     5881 gcggccgttg ttctccggac gcgcgtgcgg tccaatccca acaatggcgt tgttggtggc
     5941 gagaaggagc agcagagcca tggcacccgc ccaccgccac aggcggcgcc ccggcgtgct
     6001 cgctaggaaa agagcggcca gcagccaggc cggaatgaga atgggcaccc agaaggcgaa
     6061 gtagcctgga ttccagaagg tgaagaatgc cgcgtaggca aagccccaaa cgcccagcac
     6121 cgcgtagagc cggcgcagcc cccccgcact cgccatcagc gcgcctgcct gtgccggacc
     6181 ggggatgccg gtcatcgctt caaagtcgcg cagggcggca tgcgcccacc gcccgatgcg
     6241 gtagcccgcg cgggtccaga gcgagcccac gggagcgtca aaactcgccg ggcgtcgagg
     6301 cgtgaaccaa tagcgagatc gacggatgcc acgcccgcgc ggatcgcttt cgcccactcg
     6361 cgccagatag cggatcgccc cccaggcgac cgccacaacg gcccccccag ctgccagcga
     6421 gcccgccagc agaaaaagca agtcgcctcc agccggtgcg tcgccgccgg caagcgccgg
     6481 ttgcgccacg aaaacgtggc gcagcgcacg ggcatccagc agcaggttgc gccccagatc
     6541 caggctccac cagtggccac ggtgggcgta ggtcatcgcc cacgccagca cgcgcgaggg
     6601 ttcggaactc ccattggcca ggacggaagc cacggtgtag gcgagtgtcc ccagtcccag
     6661 cgtggtggcg aggtacactc cgatcccctg ccagcggggc cgtccaggcg aaagccagag
     6721 cgccaacacg gccgccggcg ccaccagcgc atggctttgg tggagcagca cggctaggag
     6781 gtgcagcccc cccaacaccc ccgcccaccg caggtagctc gcgcggctct ccatgctccg
     6841 ggccccaagc aggaccgcca ttgctcccag cacccccgcc aacaccacca cccccgcgat
     6901 gttgacccgg ccatccgtgc tgcaggtcca atagccatac gaaaggccca gcccggcgcc
     6961 tgccagcaaa ccggctcccc ggtggcggca gacatgcccg gcaatccagg cgaagaggac
     7021 aacccccgcg gcacccagga gggcattcat cccctggagc gcatcaaggg tgcccacctg
     7081 caggccggca gcgcggaacg cgcggaacgc acccaggccg agcgcgttga aaagaaggtg
     7141 atgctgatgg aagagccagg agtactcgcc cgtgcgcgtg aagcgctcga tctggagcgc
     7201 gtaggagaga gagtcgaacg tgtagtcgcg cgaagccgtc agccggtaga gaagagcgaa
     7261 gaggagaaag agataggcgt atccccacgc cccggcgcca gcgcgcgcgc atcctgaaag
     7321 agcgccccgg gcacgcgcca tcgcctggag gaggcggctg tttcctgggg gaggtcggcg
     7381 catggctagt gggggcgcag caacgtgccc gggcgcaccg cgacggtctc gggcagcgag
     7441 cgcagcacga gtcgggccgg ataggcggat ccagaaagcg gaagcgtggt gacgtgaacc
//
LOCUS       DAOSCV010000071         7494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6369_length_7494_cov_3.723888, whole genome shotgun sequence.
ACCESSION   DAOSCV010000071 DAOSCV010000000
VERSION     DAOSCV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6369_length_7494_cov_3.723888"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLY56_02940"
     CDS             <1..352
                     /locus_tag="PLY56_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HOJ20464.1"
                     /translation="AWLEGFLKRSGLLLLHDKELWQVLDTWLTELSAESFAAVLPLLR
                     RTFATFPAPERRQMGERVRLGKAAGSQVPGMDAGFDAERAVSVLPLAARLLGIDWPPA
                     GAAARNAGDRGEAS"
     gene            474..1619
                     /locus_tag="PLY56_02945"
     CDS             474..1619
                     /locus_tag="PLY56_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006097999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ20465.1"
                     /translation="MAITPDERLRRWRLVLGSGQADGVGMELTGVDRAIDQALEALYD
                     SDRSGGLGSSAPSVARWLGDIRTYFPKSVVRVMQRDALERLNLRQMLLEPELLEAVEA
                     DVHLVSTLLSLKSVLPGKTKETARIVVRRVVEDLERRLAAPTRQAIQGSLNRAARNRR
                     PRYSEMDWHRTIRANLKHYQPEYRTIIPETRIGYGRKRSLLREIILCVDQSGSMAASV
                     VYAGVFGAVLASLPAVKTHFVAFDTAVVDLTEELHDPVELLFGTQLGGGTDINRALAY
                     CQGLITRPMETILILLSDLYEGGDEEEMLKRAAALVNSGTQVIALLALSDDGRPAFHH
                     DIASAMAGMGIPAFACTPDLFPELMAAAINRQEIGQWAAKHDIVTAQ"
     gene            1659..2306
                     /locus_tag="PLY56_02950"
     CDS             1659..2306
                     /locus_tag="PLY56_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20466.1"
                     /translation="MADWRWARGLLAGAVAVLAPLLLGCHDSSNNRVEETHSGPRQVE
                     LQTHLEWSEGSTQLYAIIGAGWPPSGLQRTSWQEGTNYSIEPRVSIKSVRLLDDGMTL
                     CLTTAEQAPGTRYQVRYTPKVSFPAPPLEGFPQNPVLTAVAVDATHVVVVPDTHHQER
                     VDWADASSYRFEPPLAVHSAQVSEDGRLVRLTTAEQEASASYKLRVGEIATPVVR"
     gene            2605..3618
                     /locus_tag="PLY56_02955"
     CDS             2605..3618
                     /locus_tag="PLY56_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20467.1"
                     /translation="MSTIGFGVIGCGGISNVHLTALSKRDDARIVCVSDINPDAARGQ
                     AEKYGAGKWVTDANELLAMPEVNAVVVCTPTFLHPEYVIAAAKAGKHVLCEKPIAMTL
                     EDADRMIAACDEAGVFFMIAFVRRFCNEWLKLREIIQSGVLGRPVLWRGVSGSSGPRS
                     PWFLDAQKGGGPFIDGCVHDWDFARFTFGEPEVVLASVRHMKEGDSTSLDTGSAIVRF
                     QSGDELMRSWTWGLPGTACRTQSLSDVIGPKGALRFPGAPAQGSKTNSFILTDGEGVE
                     HALEYEHTIGQEWFDAQMDHFVTCIKEGRQPSVTGLDGKKALAIALAVLEAGRTHKPV
                     VLG"
     gene            3695..4156
                     /gene="nikR"
                     /locus_tag="PLY56_02960"
     CDS             3695..4156
                     /gene="nikR"
                     /locus_tag="PLY56_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOJ20468.1"
                     /translation="MAYPASCHRGRKQVVNGVERFGVSMAPDLLSRFDEVIAARGYTN
                     RSEAIRDIVRDYLIEQQWESGEGEVIGTLTLVYDHHEHDTMEQLTALQHHYHEAILST
                     LHIHLDAHHCLEVIVVRGSSTDIRRLADKVITTRGVKHGKLTCTTTGQGLA"
     gene            4269..4868
                     /gene="pyrE"
                     /locus_tag="PLY56_02965"
     CDS             4269..4868
                     /gene="pyrE"
                     /locus_tag="PLY56_02965"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ20469.1"
                     /translation="MTDELYHQVFACGCIRFGEFKLRSGLLSPFYVDLRVLVSCPQTL
                     RALGEEMAQLLKQLAPDRIAALPYAGLPIGVAASIASDIPLIYPRKEAKGYGTARLIE
                     GSFQPGERVVVVDDVITDGATKIEAIKPLEEAGLVVKDVVILLDREQGGDRVLARHGY
                     TLHSLMRLTPTLDALVRLGHITDEQRQAALRFVAEHQFS"
     gene            5019..5768
                     /locus_tag="PLY56_02970"
     CDS             5019..5768
                     /locus_tag="PLY56_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ20470.1"
                     /translation="MHFLEYFAIIERDHTIQNPSSPEKIRLLADYCQVRHGLRVLDVG
                     SGKGYLLCTWAKEWQIEGTGLEINPWFIAEARARAQEAGVTDRVAFIEGPALDFSPEP
                     GGYDIVLCIGASFALQDFDHAIPWMLAALKPDGVLAIGEPFFHAPLPPEVAAKVDPEL
                     ARLHDLAGTVAAFEEHGLSLDGFIAASLDDWDRYESPHWRAAREWARANPDHPDREEF
                     LRRVRHDKEAYFQWQRQYLGWGIFVGRRASF"
     gene            complement(5818..>7494)
                     /locus_tag="PLY56_02975"
     CDS             complement(5818..>7494)
                     /locus_tag="PLY56_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ20471.1"
                     /translation="EGRTLEAVLEKIDEAARDGADIVSLPMECVKTEGEPIPGPISQA
                     IAARAAKHKIWVVGNIREAEGEKRFVTSFLCDRAGQIVGKYRKSHKMPDETMDLGDDL
                     PVFQTDFGKIAMRIGSDRFFPDIDHVYTVKGASLILWAQQPEPVEDEFSQDFPSEGRA
                     TDYNVHIACSRYASAGQGWITNKFPPYCGMPIGRAYVINREGQRVACTPRMGGGVATA
                     ALPFDSFGPGRQAPRSKAFAALTAPVQVPERKQWKKREVRVTAIEAHLGIEELLAALD
                     EAGRIGTDIACTYEFVWISGPDPERIARMTETARANVARVAEKAKQYGMYVILAGVVE
                     RIERNEAILFGRDGQVVGRYYKIAKTHPEMICGEETPVFETDFGRVAMRICADEWMVE
                     LDRCYGLKGADILFVPTQSWGPDALFRDQRDISRAMDAGLFHVQATHPSSEVRHRSLI
                     IEPTGAVVARSEYRMNSIVSAVIDLDNDRPLRYIRDYKPHTPGGYMAQYQPDRMPAAA
                     NDLRETILRQRRPELYGVLASQTETQATSDNPLMEEWGAPPGLLPLLRYS"
BASE COUNT         1234 a         2479 c         2481 g         1300 t
ORIGIN      
        1 cgcatggctc gaaggcttcc tcaagaggag tggcctgctt ctgctgcacg ataaggagct
       61 ctggcaggtg cttgatacct ggctgaccga gctctccgca gagagcttcg cggcggtgct
      121 cccgctcttg cggcggacct ttgcaacctt ccctgccccg gagcggcggc agatggggga
      181 gcgcgttcgc ctgggaaagg cggcaggctc ccaggtcccc ggcatggacg cgggcttcga
      241 tgccgagcgc gcggtgtccg tcctgccgct cgccgcccgg cttctgggca tcgactggcc
      301 gcccgccggc gccgccgcga ggaatgctgg agaccgcggg gaagcgtcgt gaagtttggt
      361 gacttcgaga gccgggcgcg cgcgctggag cctctccacc cgccgcccgg cgtctggatt
      421 ggcttgcgcc cgggaggcgg gtagcgggag cagagattgg agtggtcggc gagatggcga
      481 taacaccgga cgagcgtctg cggcggtggc ggctggtcct cgggtcgggc caggcagatg
      541 gggtgggcat ggagctgaca ggtgtggatc gggcaatcga ccaggcgctg gaagccctgt
      601 acgactctga ccgcagtggt gggttgggca gctccgcgcc gagtgtggcc cggtggctgg
      661 gcgatatccg cacctacttc cccaagtcgg tggtgcgcgt gatgcaacgc gatgccctcg
      721 agcgcctgaa cctccggcag atgctcctgg agccggagct gctggaagca gtggaggccg
      781 atgtgcacct cgtctccacc ctcctctcgc tgaagagcgt gctcccgggc aagacgaagg
      841 agaccgcgcg catcgtggtg cgccgtgtcg tcgaggatct ggaacgccgc ctggcagcgc
      901 ctacgcggca ggcgatccag ggcagcctga atcgcgccgc acgcaatcgc cgcccgcggt
      961 attcggagat ggactggcac cgcaccatcc gcgctaacct gaagcactac cagccggagt
     1021 accgcaccat catccccgag acgcgcatcg gctacggacg gaagcgttcc ctgctgcgcg
     1081 agatcatcct ctgcgtggat cagagcggct cgatggccgc ctcggtcgtc tacgcgggcg
     1141 tcttcggggc ggtcctcgcc tcgctgccgg cggtcaagac gcactttgtc gctttcgata
     1201 cggcggtcgt cgatctcacc gaagagctgc atgacccggt agagctcctc tttggcaccc
     1261 agctgggtgg gggcaccgat atcaaccgcg cgctcgccta ttgccagggc ctgatcaccc
     1321 gccccatgga gacaatcctc atcctcctga gcgatctcta cgaggggggc gacgaggagg
     1381 agatgctgaa gcgcgccgcg gcccttgtca actccggcac ccaggtgatt gcgcttcttg
     1441 ccctgagcga tgacggccgg cccgcgttcc accacgatat cgcgtcggcg atggccggga
     1501 tggggatccc ggcgtttgcc tgcactcccg atctcttccc ggaattgatg gccgccgcga
     1561 tcaaccgcca ggagatcggg cagtgggccg ccaagcatga tatcgtgact gctcagtagg
     1621 agcgttggaa acaccattgt tttggagaga tgcgcatcat ggcagattgg agatgggcga
     1681 gagggttgct cgcgggggcc gtggccgtgt tggcacccct tctcctgggg tgtcatgact
     1741 ccagtaataa ccgggtggag gagacacact ccggccccag gcaggtggag ttgcagacgc
     1801 acctcgaatg gtcggaaggg agcacgcagc tctacgcgat catcggcgcg ggctggccgc
     1861 cctccggtct ccagcgaacc tcgtggcagg aaggcacgaa ctactccatc gagccccgcg
     1921 tgagcatcaa gagcgtccgg cttctggatg acggcatgac cctctgcctc acgacggctg
     1981 agcaggcacc gggcacgcgc tatcaggtgc gctatacccc gaaggtgtcg ttccccgcgc
     2041 cacccctgga aggctttcca cagaacccgg tgctgaccgc cgttgccgtc gatgccacgc
     2101 acgtggtcgt cgtacccgac acgcaccatc aggagcgcgt ggactgggcg gatgcgagta
     2161 gctaccgctt tgagcctcca ctcgccgttc actcggccca ggtcagcgag gatggccggc
     2221 tggtgcgcct gacaacggcc gagcaagagg catccgccag ctacaagctg cgcgtgggtg
     2281 agatcgcaac gcccgtcgtc cggtaggcgc gagcgcggca ggccggaccc cttccgaaat
     2341 cgcgcgggcg cgccgcgtct ttagggagca ggcaacgctt cccgtgttac atccgccgcg
     2401 gtttggttgt caaagggaga tggcaaggcg gtggcatcac gcgcgggaga cgtagggcac
     2461 accgcgagcc acctgcccga gctatgagat tgaaccggaa cccgtccaca cgcggctctt
     2521 cgcgccacgc tcttccccct ttctggcgtg actcggcgga accgcgcccg cgcggcgggt
     2581 taccaggccg aaaggaatca ctcgatgagc accatcggat tcggcgtgat tgggtgtggc
     2641 ggtatcagca acgtgcacct caccgccctc tccaagcgtg acgatgctcg catcgtctgc
     2701 gtctcagaca tcaacccgga tgccgctcgc ggccaggcgg agaagtacgg cgccggcaag
     2761 tgggtaaccg acgccaacga gctcctggcg atgccggagg tcaacgccgt cgtcgtctgt
     2821 acgcccacct tcctccatcc ggagtatgtg attgccgcgg ccaaggcggg caagcacgtc
     2881 ctctgcgaga agccgattgc catgacgctg gaggatgccg accggatgat cgccgcgtgc
     2941 gacgaggcgg gggtcttctt catgatcgcg ttcgtccgcc gcttctgcaa cgagtggctg
     3001 aagctgcgcg agatcatcca gagcggcgtt ctcggccggc cggtcctctg gcgcggcgtc
     3061 tccgggagct ctggcccgcg cagcccctgg ttcctcgatg cccagaaggg cggcggcccc
     3121 ttcatcgatg gctgcgtcca cgactgggac ttcgcgcgct tcaccttcgg cgagccggag
     3181 gtggttcttg cctccgtccg gcacatgaag gagggcgact cgacctccct cgataccgga
     3241 agcgccattg tccgcttcca atccggggat gagctgatgc gcagctggac ctggggcctc
     3301 cccggcaccg cgtgccgcac gcaaagcctc tccgatgtca tcgggcccaa gggggctctg
     3361 cgcttcccag gcgctccggc acaaggctcg aagaccaaca gcttcatcct cactgatggc
     3421 gaaggcgtcg agcacgcgct ggagtacgag cacaccatcg gccaggagtg gtttgacgcc
     3481 caaatggacc acttcgtcac ctgcatcaag gagggccggc agccctcggt caccggcttg
     3541 gatggcaaga aggcgctcgc cattgccctg gcggtgctcg aggcggggcg cacccacaag
     3601 ccggttgtgc tcggctgaat ctgacgatct cggatgggga gcctgggatg ggagcccccg
     3661 cttccattcc aggctcctct ttatccggga tgggatggcg tatccagcat cctgtcatcg
     3721 agggagaaaa caggtggtga acggcgttga gcgcttcggg gtctccatgg cccccgatct
     3781 tctgagcagg tttgacgagg taatcgcggc acgcggctat accaaccgct ccgaggcgat
     3841 tcgcgacatc gtgcgcgact acctcatcga acagcagtgg gaatccggcg agggagaggt
     3901 gatcggcacg ctcaccctcg tgtacgacca ccacgagcac gacaccatgg agcaactcac
     3961 ggcgctgcag caccactatc acgaagcgat cctctccacc ctgcacatcc acctcgatgc
     4021 gcatcactgc ctggaggtga tcgtggtccg cggctcctca accgatattc gccgcctggc
     4081 cgataaggtg atcaccaccc gcggcgtcaa gcacggcaag ctcacctgca ccaccaccgg
     4141 ccagggcctc gcctgacggg cttcaattgc tccgatggcg cgtccccgcg tcgcgcaccc
     4201 aggatgatac cgcgcggaag ggtagccccc agagggcgtg gaatctgcat gggtgaggat
     4261 tactgaccat gaccgatgaa ctgtatcacc aggtatttgc gtgcgggtgc atccgctttg
     4321 gcgagttcaa gctccgctcg gggctcctct cgcccttcta cgtggatctc cgcgtgctcg
     4381 tctcctgccc gcaaacgctg cgggcgctgg gcgaggagat ggcgcaactg ctcaagcaac
     4441 tcgcgccgga ccgcattgcc gcccttcctt acgccgggct ccccatcggg gtggccgcca
     4501 gcatcgccag cgatatcccg ctcatctacc cccgcaagga agcgaagggg tatggcaccg
     4561 cgcggctgat cgagggctcg ttccagccgg gcgagcgcgt cgtcgtcgtg gatgacgtga
     4621 tcaccgatgg cgcgaccaag atcgaggcga tcaagccgct tgaggaagcc ggattggtgg
     4681 tcaaggatgt cgtcatcctg ctcgaccgcg agcagggcgg cgaccgggtg ctggcgcgcc
     4741 acggctacac gctgcactca ctgatgcgcc tgacgccaac gctcgatgca ctcgtgcgcc
     4801 tggggcacat caccgacgaa cagcgccagg cggcgctgcg cttcgtcgcc gagcaccagt
     4861 tctcgtaggc atccggcgac acggaagaag ggctcctcct gctcgcgcca gcgttcgctg
     4921 ccctgttggc ccggtttcac ggccacctgc tccgcgatag acactctgtt gagcaacacc
     4981 ggcccggcac gcgataggga ggatgtagga ggagtctcat gcatttcctt gagtatttcg
     5041 ccattattga gcgtgaccac acaatccaga acccctcgtc gccagagaag atccgcctgc
     5101 tggccgacta ttgccaggtg cgccacgggc tgcgcgtgct cgatgtaggg agcggcaagg
     5161 gttacctcct ctgcacgtgg gcaaaagagt ggcagattga gggcaccggc ctggagatca
     5221 acccctggtt catcgcggag gcccgggcgc gcgcccagga agccggcgtg accgaccggg
     5281 tcgccttcat cgaggggccg gcgctcgact tctcgcccga gccaggtggc tacgacatcg
     5341 tcctctgcat cggtgcctca ttcgcgcttc aggatttcga ccatgccatt ccgtggatgc
     5401 tcgccgcgct gaagccggac ggagtgctcg ccatcgggga gccgttcttc cacgcgcctc
     5461 tgccgccgga agtcgcggcg aaggtcgatc cggagctcgc cagactgcac gacctggctg
     5521 gcacggtcgc cgcgttcgag gaacatgggt tgagcctcga cggcttcatc gcggcgtcgc
     5581 tggatgattg ggaccgctac gagagccctc actggcgggc agctcgcgaa tgggcgcgcg
     5641 cgaacccgga ccacccggat cgcgaggagt tcctccgccg ggtacgccac gacaaagagg
     5701 cctacttcca gtggcagcgg cagtatctcg gctgggggat cttcgtcggc cgccgggcct
     5761 cgttctgact gtaacgggcg gcccgcatcc cgcggaccgc ccgtggcatc gcgcctgtta
     5821 gctatagcgc aacagtggca agaggccggg gggcgcgccc cactcctcca tcagtgggtt
     5881 gtcactagtg gcctgcgttt ccgtttggga ggcgagaacg ccgtacagct ccgggcggcg
     5941 ctggcgcagg atcgtctcgc gcagatcgtt cgccgcagcg ggcatccggt cgggctggta
     6001 ctgagccatg tagccgccgg gcgtgtgcgg cttgtagtcg cggatgtagc gcaagggccg
     6061 gtcgttatcc aggtcgatca ccgcggagac gatgctgttc atccgatatt cggagcgcgc
     6121 gacgacggcg ccggttggct cgataatcag gctgcggtgg cggacctcgg acgaggggtg
     6181 cgtcgcctgc acatggaaga ggccggcatc catggcgcgg gagatgtcgc gctggtcgcg
     6241 gaagagtgcg tcggggcccc agctctgggt cggtacgaag aggatatcgg cgcctttcag
     6301 gccatagcag cggtcgagct ccaccatcca ctcatcggcg cagatgcgca tggcgacgcg
     6361 cccgaaatcg gtctcgaaga cgggcgtctc ctctccgcag atcatctccg ggtgcgtttt
     6421 ggcgatcttg tagtagcggc caaccacctg accatcgcgc ccgaagagga tcgcctcatt
     6481 ccgctcgatc cgctccacaa cgccggcgag gatcacgtac atgccgtact gtttcgcctt
     6541 ctcggccacg cgggcgacgt tcgcgcgtgc ggtctccgtc atccgggcaa tccgctccgg
     6601 atccgggccg gagatccaga caaactcgta ggtgcacgcg atgtcggtgc cgatccgccc
     6661 cgcctcgtcg agcgccgcca gcagctcctc aattcccagg tgcgcctcga tggcagtgac
     6721 gcggacctcg cgcttcttcc actgcttccg ctccggcacc tggaccggtg cggtgagcgc
     6781 ggcgaacgcc ttgctgcgcg gcgcctgccg gcccggcccg aaggagtcga aggggagggc
     6841 cgccgtcgcc acgcccccac ccatgcgcgg cgtgcacgcg acgcgctggc cctcacggtt
     6901 gatgacgtag gcccgcccga tcggcatccc gcagtaaggc gggaacttgt tcgtgatcca
     6961 cccctgcccg gccgacgcgt agcgagagca ggcgatgtgg acgttgtaat ccgtggcgcg
     7021 cccttccgac gggaagtcct ggctgaactc atcctccacc ggctctggct gctgtgccca
     7081 gaggatcagg ctggcgccct tcacggtgta aacgtggtcg atatcgggga agaagcggtc
     7141 ggacccaatc cgcatggcga tcttgccgaa gtcggtctgg aagacgggca ggtcatcgcc
     7201 caggtccatc gtctcatccg gcattttatg cgacttgcgg tacttcccga cgatctggcc
     7261 ggcgcggtcg cacaggaagg atgtgacgaa gcgcttctcg ccctccgcct cacggatgtt
     7321 gccaacgacc cagatcttgt gctttgcggc gcgggccgcg attgcctggc tgatcggccc
     7381 gggaatcggc tcgccctccg tcttcacgca ctccatcggg agcgagacga tgtcggcgcc
     7441 gtcgcgcgcg gcctcgtcga tcttctccag aacggcttcg agcgtccgcc cctc
//
LOCUS       DAOSCV010000072         7354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6494_length_7354_cov_3.219345, whole genome shotgun sequence.
ACCESSION   DAOSCV010000072 DAOSCV010000000
VERSION     DAOSCV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6494_length_7354_cov_3.219345"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1511
                     /locus_tag="PLY56_02980"
     CDS             234..1511
                     /locus_tag="PLY56_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20472.1"
                     /translation="MRKRFLCLGIAGLCLGLVAGPAAGEVESEEAPAERRFTLRTGLK
                     SSLLISRGPKDPLLPSERDEATALWRLRFDLTARLSEETTAAVAYEHRARVVSEGRQT
                     ALSLGILPPAAPAPYRIWQLDAELASAGDSFEYRHELDRAYVAKRIPRGELTVGRQAV
                     GWGRGVLFSAVDLFAPFSPLEVDREWRRGVDAVRAEVRLSDTTSLDAVAAFGESLAQS
                     AFVGRLRGYRGRVDAEVIFGKRARDVMAAGTVSAAVGDAEAHAELVIFNTPEAFPDSG
                     LFGADHLVPKAVVGGSYTFSVGQGLTLLGEYHYSGFGAGSFAEAIQRLLDPQFRERFL
                     RGDTQIVERQAAALQASYPVNEALSSSLLWLHSLQDGSGVVVPALSWNISDQLGLTGQ
                     LFFPYGRGPLNGVPRTEYGNSDLTGFVQLRLDY"
     gene            complement(1532..2596)
                     /gene="pheA"
                     /locus_tag="PLY56_02985"
     CDS             complement(1532..2596)
                     /gene="pheA"
                     /locus_tag="PLY56_02985"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOJ20473.1"
                     /translation="MTIQDLRARIDAIDAEVLSLLSERARLAMEIGHRKADRTESFFA
                     PTREKQVIQGLLRRNEGPLPEAAIRAIYREIISACRALEKPLRVAYWGPPASFTHVAS
                     LQRFGSLTEYLACESVSDVFATVSKRQADYGVVPIENSTEGIVEYTLDTFSEFDLVIC
                     AEIYVEITHNLLSLAGSLDEVGRVYTGAQPLAQCRKWLEHHMPGVEISEVATSSRAAQ
                     LAAENPATAAIANTMAAELYHLRILQEHIEDSPHNRTRFLVVGHTETPPSGHDKTSVL
                     MSVKHRAGSLFRALAIFDKYDVNLTMIESRPSKRTPWEYIFFIDFQGHCRDPHIQKAL
                     ERLEQEVQYVKVLGSYPEAE"
     gene            2794..3687
                     /gene="accD"
                     /locus_tag="PLY56_02990"
     CDS             2794..3687
                     /gene="accD"
                     /locus_tag="PLY56_02990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOJ20474.1"
                     /translation="MAPNGWFHRYPRIKGGGEPKAQHVPEGIFEKCPKCGEALLVKEL
                     EKNLKVCRKCGYHFRLSVRERIELLTDPGSFQEIATGLSSVDILEFPEYPEKLAKGRA
                     NTGLNDGMVVGSARIAGWPVILGVADFRFMGGSMGSVYGERVTRALEEALQRRVPAIL
                     VCASGGARMQEGLVSLMQMAKTSAAVGRLNEAGVPYITVLTDPTTAGVFASFASLGDI
                     IIAEPGALMRFAGERVAAQAGPAKQPPPNFQTAEFYLEHGAIDLIVHRKDLQDTLSRI
                     LTFCCHGRLAREGGEALAAVR"
     gene            3766..4737
                     /locus_tag="PLY56_02995"
     CDS             3766..4737
                     /locus_tag="PLY56_02995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOJ20475.1"
                     /translation="MATIRNLLDFEKPLHELEEQVQELKRFTAAQGIDRSQEIAALES
                     QLRAVTKRIYSQLAPWDKVQLARHPARPYTLDFIRMIFDDFLELHGDRTYGDDPAIVG
                     GLAVLNGEPVMVIGHQKGRDIKERSLRNFGSARPEGYRKAVRLLKLAQKTGRPVVSFV
                     DTPAADCSVGAEERGISEAIARSMMEMSGIRVPVVVAITGEGGSGGAIATAVGDRILM
                     LEHAIYSVIPPEGCAAILWRDPSKAPQAAAALKLTAKDVHRLGIVDRVIPEPLGGAHR
                     DASGAAQLLKQAIVESMDELRGLSPNELLERRYKKFRNMGRYLEMAG"
     gene            complement(4742..6682)
                     /locus_tag="PLY56_03000"
     CDS             complement(4742..6682)
                     /locus_tag="PLY56_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20476.1"
                     /translation="MDDPNLYIKPGVIGKVVATESRPATAAEFYFWTRSDNLAIEVGS
                     IVVATSHEGMTFGLLDEPQRYSDLHSFMDDYIAHDFGDVTTDAPTAPPDVLVFKAKVL
                     RTLVLDPVAGSWSERMRPTPPGNVYFPTPQGIEFAMYADRIEPERRIPAGLFSNSDGS
                     TTPIHIDEEFLLGMEGAHLNITGVSGLATKTSYIEFLLRSLFAHSRRSIAVVCFNVKG
                     PDLLFLDKPNVPADPALRARYEAHGVKMMPEEQLRMYEALGLPPTPFEQVHIFAPYKA
                     DGQTLNTTRTHPEVSGNVRPFVWGLKDVIGDIRTLVGRDDIDEKADAFLAFVRDRWVQ
                     KEEVLSFDELELKLNTIIEDLEDREVSQYHSHHLATLRKMRNRLVNIPARCPGLITRG
                     RVSQGDDIPLEQLQDRDIFVIDVSGLGTTAQDLVFQKVIGRLSRLKERDELPVDHVIV
                     FVDELNKFAPRQGETPLKRGLVEISARGRYIGFVLFGAQQFRSKVDAEVIGNTATSLY
                     GRMDAEEIIEPPYRMFSETVKEKLLSLEKGELLLRHPHFKQPIWVRFPVPPVLRGKDG
                     LVLWPSRRSNSIERVYDELKALDPSAEIPVEEMRRRLDALDDEAIQRILEYVRRKYNP
                     RGIISLWQVFKSGVRAETGRGD"
     gene            7034..>7354
                     /locus_tag="PLY56_03005"
     CDS             7034..>7354
                     /locus_tag="PLY56_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ20477.1"
                     /translation="MRAVKFFGRERVAVREYPNPKPQEGQVVVRIRASALCGSELHAY
                     RGEQELESNPGHEPAGVVVDASRSRRWRDGDRVGIHAVWGCGTCEWCQRGVFTFCDEK
                     RFCPG"
BASE COUNT         1230 a         2267 c         2529 g         1328 t
ORIGIN      
        1 ggacggccac gtgaagtggt ataacgagca gggcgtgcgc agcggccagg tgggggaagt
       61 ctactggcaa tagccgtcgg cagccgggtg ccaggggcgc cgctgttggg cctcacggct
      121 tgccgaggcg atatgcccgc ggcacccagg taatcacagg gcgggcgacg ggtgggtgac
      181 catgcaggag ggcgccctcg cgctgtgtgg ggtgttcatc ggaagggagc gcggtgagga
      241 agcggttcct ctgcctcggt atcgcgggcc tgtgcctcgg gctggtcgcg gggccggccg
      301 ctggcgaggt ggagagcgag gaagcgcctg ccgagaggcg ctttaccctg cgcacgggac
      361 tcaagtccag cctgctgatc tcgcgcgggc cgaaagatcc gctgctccct tccgagcgtg
      421 acgaggccac cgcgctctgg cggcttcgct ttgacctgac ggcccggctc tcggaagaga
      481 ccactgccgc cgtggcctac gagcatcgcg cgcgcgtcgt ctcagagggc cggcagaccg
      541 ctttgagcct gggcattctc ccgccggcag cgccggcgcc ctaccgcatc tggcagctcg
      601 atgccgagct cgcctcggcg ggcgattcct tcgagtaccg ccatgagctc gaccgcgcct
      661 acgtggcgaa gcggatccca cgcggggaac tcacggttgg ccggcaggcg gtgggttggg
      721 ggaggggcgt cctcttcagc gccgtggatc tcttcgcgcc cttctcaccg ctggaggtgg
      781 atcgcgagtg gcgccggggc gtggatgccg tgcgcgccga ggtgcgcctc tcggatacca
      841 catcgctcga cgcggtagcc gcttttggcg agagcctggc ccaatccgcg ttcgtgggcc
      901 ggctgcgcgg ctaccgcggt cgcgtggatg ccgaagtgat ctttgggaag cgcgcgcgcg
      961 acgtgatggc ggccggcacg gtctctgccg ccgtgggcga tgccgaggcg cacgcggagc
     1021 tggtgatctt caacaccccg gaggcgtttc cagactcggg cctcttcgga gcggaccacc
     1081 tcgtcccgaa ggcggtggtg ggtggctcct acaccttcag cgtggggcag gggctcacgc
     1141 tcctgggcga gtaccactac tccggcttcg gagcggggag cttcgccgag gcgatccagc
     1201 gcctgctgga cccacagttt cgcgagcgct tcctccgtgg cgacacccag atcgtggagc
     1261 gccaggcggc agcgctccag gcatcctacc cggtgaacga ggcgctctct tcgtcgctcc
     1321 tctggctgca tagcctgcag gatggctccg gcgtggtggt accggcgctc tcctggaaca
     1381 tcagtgatca actcggcttg acgggccagc tcttctttcc ctacggccgc ggccccctca
     1441 atggagtgcc ccgcaccgaa tacggcaaca gcgatctcac cggcttcgtc cagcttcgtt
     1501 tggattactg aacgccgccg gtccgggctg gctactccgc ctcaggatag gagccgagga
     1561 ccttcacgta ctgtacttcc tgctccaggc gttccagggc tttctggatg tgcggatcac
     1621 ggcaatggcc ctggaagtcg atgaagaaga tgtactccca cggggtgcgc ttgctggggc
     1681 gcgattcgat catcgtcagg ttcacgtcgt acttgtcgaa gatcgcgagc gcccggaaga
     1741 gcgacccggc gcgatgcttc accgacatca ggaccgaggt cttatcatgc ccgctcggcg
     1801 gggtctccgt gtgcccgacg acgaggaagc gggtgcggtt gtggggcgag tcttcaatat
     1861 gctcctgcag gatgcgcagg tgatacagct cggcagccat ggtgttggca atcgctgccg
     1921 tggcggggtt ttcggcggca agctgcgccg cgcgcgagct ggtggccacc tcggagatct
     1981 ccacgccggg catgtggtgc tccagccact tgcggcattg ggcgagcggc tgcgcgccgg
     2041 tgtatacccg gcccacctca tcgagcgacc ctgccagcga taggaggttg tgtgtgatct
     2101 ccacgtagat ctccgcgcag atcaccaggt cgaactcgct gaaggtatcc agtgtatact
     2161 cgacgattcc ttctgtagag ttctcgatgg gcaccacgcc atagtctgcc tggcgcttgc
     2221 tcacggtggc aaagacatcc gagaccgact cgcacgcgag atactcggtg agagagccaa
     2281 agcgctggag gctggccacg tgggtgaagc tggcaggcgg cccccaatag gcaacacgca
     2341 ggggcttctc cagtgcccgg caggcggaga tgatctcgcg gtagatggcg cggatcgcgg
     2401 cttcaggcaa ggggccctcg ttcctccgca gcaacccctg gatcacctgt ttctcccggg
     2461 tgggggcaaa gaagctctcc gtgcgatctg ccttccggtg tccgatctcc atcgccagac
     2521 gggcgcgctc gctcaacagc gaaagcacct ccgcgtcgat ggcatcgatg cgggcgcgta
     2581 ggtcttggat cgtcatgcgg ctctcctcga aacaaagggg tggtcttctg gcatccaaaa
     2641 atcaagctct cccattatat cacgcaagga aaggaaaagg aaacccgggc gcgcaggtcg
     2701 tatatgggtt ccgtatacat cgcaaatcag aagactctac atctgcacca cagttccgga
     2761 tctcacccgg ctagtgccca ggaggaagtg tgcatggcac ctaatggttg gttccaccgt
     2821 taccccagga tcaaaggggg cggcgagccc aaggcccaac atgttccgga aggcatcttc
     2881 gaaaagtgtc ccaaatgcgg cgaggcgctg ctggtcaagg agctggagaa gaacctgaag
     2941 gtgtgccgca agtgcggcta ccatttccgg ctgagcgtgc gagagcggat cgagctcctc
     3001 accgaccccg gcagctttca ggagatcgcc accgggctgt ccagcgtgga tattctcgag
     3061 tttcccgaat atcccgagaa gctggcaaag ggccgcgcga atacgggctt gaatgatggc
     3121 atggttgtcg gaagcgcgcg tatcgccggg tggccagtca tccttggggt ggcagacttc
     3181 cgcttcatgg ggggaagcat gggctcggtg tatggcgagc gcgtgacgcg tgccctggag
     3241 gaggcgctgc agcggcgcgt gccggcgatc ttggtctgtg cctcgggcgg cgcgcgcatg
     3301 caggagggcc tcgtttcgct gatgcaaatg gcgaagacga gtgccgcggt cgggcggttg
     3361 aacgaagcgg gcgttcccta tatcacggtg ctcacggatc cgacgacggc gggcgttttc
     3421 gcgagcttcg cgtcgcttgg cgatatcatc attgccgagc caggtgccct gatgcgtttc
     3481 gcgggagagc gcgtggcggc ccaggccggc cctgccaagc agccgccccc caacttccag
     3541 accgccgagt tctacctgga gcatggtgcc atcgacctga ttgtgcatcg caaggatctc
     3601 caggataccc tttcgcggat actgaccttc tgctgccacg ggcgattggc ccgcgagggc
     3661 ggcgaggcgt tggccgcggt gcgctgagac ttctccggag gcatcccgga ggaggttttg
     3721 ctccacgcgg gtgtttttcg gatcggcccg atgaggagtg tgggtgtggc cacaatacgc
     3781 aacctattgg actttgagaa gccactgcac gagcttgagg aacaggtaca ggagttgaag
     3841 cggttcacgg ctgcgcaagg gattgatcgc agtcaggaga tcgcagcgtt ggaatctcag
     3901 ctcagggcgg ttactaagag gatctattcg cagcttgctc cgtgggacaa ggtgcagctg
     3961 gcgcggcatc ccgcccggcc ctacacgctc gatttcatcc gcatgatctt cgacgacttc
     4021 ctcgagctgc acggcgaccg cacctatggc gatgacccgg cgattgtcgg cgggttggcg
     4081 gtgctcaatg gcgagcccgt gatggtgatc ggccatcaga agggtcgtga cattaaggag
     4141 cgaagcctgc gcaacttcgg aagcgcccgg ccggagggct atcgcaaggc ggtccgcctc
     4201 ctcaagctgg cgcagaagac aggccggccc gtggtctcct tcgtggatac cccggcagcc
     4261 gattgtagcg tcggcgccga ggagcgcggg atcagcgagg cgatcgcacg cagtatgatg
     4321 gagatgtctg gcatccgtgt gccggtcgtg gtggctatca ccggcgaggg cggcagcggc
     4381 ggcgcgattg ccacggccgt gggcgaccgc atcctgatgc tggagcacgc catctactcg
     4441 gtgatccccc ccgaggggtg tgccgctatc ctctggcgcg atccgagcaa ggccccccag
     4501 gctgccgcgg ccctcaagct caccgctaag gatgtgcacc gactgggcat cgtggaccgc
     4561 gtcatccccg agcctctcgg aggggcccac cgtgatgcct ctggcgcagc gcagttgctc
     4621 aagcaggcaa tcgtcgagag catggatgaa ctgcgcggcc tctcgcccaa cgagctgcta
     4681 gagcggcgct acaagaagtt ccgcaatatg ggccgctacc tggaaatggc cggctgatcg
     4741 ctcagtcgcc acgccccgtt tcggcccgga cgccggattt gaacacctgc cagaggctga
     4801 tgatgccgcg cgggttgtac ttccggcgca cgtactccag gatgcgctgg atcgcctcgt
     4861 catccagcgc atccagcctc cggcgcatct cctccacggg aatctccgcc gagggatcca
     4921 gcgccttcag ctcgtcgtag acacgctcga tggagttcga ccggcgcgac ggccacagca
     4981 ccagcccatc cttcccgcgc agcactgggg gcacggggaa gcggacccag atcggctgct
     5041 tgaagtgcgg gtggcgcagg agcagctcgc ccttctccag agagagcagc ttctccttga
     5101 ccgtctcgga gaacatccgg tagggcggct cgatgatctc ctccgcgtcc atgcgcccgt
     5161 agagtgaggt cgccgtgttg ccgatcacct cggcgtcgac cttcgaccgg aactgctggg
     5221 cgccgaagag cacgaacccg atgtagcgcc cccgggcgga gatctccacc agcccgcgct
     5281 tcagaggcgt ctcgccctgc ctgggcgcga acttgttcag ctcgtccacg aagacgatga
     5341 cgtgatcgac cggcagctcg tcgcgctcct tcagtcggct gagccgccca atcacctttt
     5401 ggaagaccag gtcttgcgcc gtcgtgccga gcccggagac gtcgatcacg aagatgtcgc
     5461 ggtcctggag ctgctccagc ggaatgtcat cgccctggct gacacgcccg cgggtgatca
     5521 acccggggca gcgggcaggg atgttcacca gccgattccg catcttgcgc agggtggcca
     5581 ggtggtggct gtggtactgg ctgacctcgc gatcctccag atcctcgatg atcgtgttga
     5641 gcttgagctc caactcgtca aacgagagga cctcctcttt ctgcacccag cggtcgcgaa
     5701 caaacgcgag gaacgcgtcg gccttctcat cgatatcgtc gcgcccgacc agcgtgcgaa
     5761 tatcgccaat gacatccttg aggccccaga caaagggacg gacgttgccg gacacttcgg
     5821 ggtgagtgcg cgtggtgttg agagtctgcc cgtccgcttt atagggcgcg aagatgtgca
     5881 cctgctcgaa gggcgtgggc ggaagcccga gcgcctcgta cattcggagc tgttcttcgg
     5941 gcatcatctt cacgccgtgc gcttcgtagc gcgcccggag tgccggatcg gcgggcacgt
     6001 tcggcttatc caggaagagc aggtcggggc ccttcacgtt gaagcagacc acggcgatgc
     6061 tgcgccgcga atgggcgaag agcgagcgga gcaggaactc gatgtaggat gttttcgtcg
     6121 ccaggccgga gacgccggtg atgttcagat gcgcgccctc catgccgagg agaaactcct
     6181 cgtcgatatg gatcggagtg gtggagccat cgctgtttga gaagagcccc gccgggatgc
     6241 gccgctccgg ttcgatccgg tcggcataca tcgcgaactc gatcccctgg ggcgtgggga
     6301 agtagacgtt ccccggcggc gtcgggcgca tgcgctcgga ccagctcccc gcgacgggat
     6361 cgagcacgag cgttcggagg accttcgcct tgaagacgag gacatccggg ggggccgtcg
     6421 gcgcgtcggt ggtcacatcg ccgaaatcgt gggcgatgta gtcatccatg aaggagtgaa
     6481 gatcagagta gcgctgcggc tcatcgagaa gcccgaaggt catcccctca tggctagtcg
     6541 cgacgacgat agagccgacc tcgatcgcga ggttgtcact gcgcgtccag aagtagaact
     6601 cggcggcggt ggcggggcgc gactcagtgg cgaccacctt cccgatcacg ccgggcttga
     6661 tgtagaggtt gggatcgtcc atgtgggttt gcctgggtct cctgggcatt cgctcgggct
     6721 acgtcgcgct cgatgggcgc ggcggcacct tgtaaagcgg ttcgctggaa gccggctgcc
     6781 ctcctgccgt tggttcggcg gtgcctgttg gggcgggacc gccggcctac aggcgccggc
     6841 ggcgataccg cggatgaacg ggacggacac ggatggggat gccacgggtg ccttggggat
     6901 gctcccattc atctgtgttt gcctgcgtca tcggcgggta gcaacccccg ggaagggaga
     6961 ggcttctcct ctcacgaagt cccgaatggc gcggttcccc ggcaaggcgc gccagggagc
     7021 gaggagaaag ctgatgcggg cagtgaagtt tttcggacgc gagcgggtgg ctgtccggga
     7081 gtatccgaac cccaagcccc aggaggggca ggtggtggtc agaatccgtg cctcggcgct
     7141 gtgcggcagc gagctgcacg cctaccgcgg cgagcaggag ctggagtcca atccgggcca
     7201 tgagcccgcg ggcgtggtcg tcgatgcgtc gcgctcgcgc cggtggcgcg atggcgaccg
     7261 ggtaggcatc cacgcggtat ggggctgcgg tacctgcgag tggtgccagc gcggcgtgtt
     7321 caccttctgc gacgagaagc ggttctgccc gggt
//
LOCUS       DAOSCV010000073         7348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6498_length_7348_cov_3.618401, whole genome shotgun sequence.
ACCESSION   DAOSCV010000073 DAOSCV010000000
VERSION     DAOSCV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6498_length_7348_cov_3.618401"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLY56_03010"
     CDS             <1..385
                     /locus_tag="PLY56_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ20478.1"
                     /translation="SPLPVAAAIASLKVIQSRGLVEHAAKMGEIMMPELARIQAKYPG
                     PLGCLTGKGLVAGIQVVKPGTKEPDPDTALAINIACMHRGVLMFAPVGGAGECIKIAP
                     PLTIPEDALRESISAFEEAVDAVLG"
     gene            complement(1069..1395)
                     /locus_tag="PLY56_03015"
     CDS             complement(1069..1395)
                     /locus_tag="PLY56_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HOJ20479.1"
                     /translation="MRTIILLTISNIFMTFAWYGHLRFKSSPLWIVILASWGIAFVEY
                     CFQVPANRIGHGQFSAAQLKVIQEVITLVVFAGFSVAYLRESLRWNHVIAFLFILGAV
                     FFMFRR"
     gene            1732..3066
                     /gene="rimO"
                     /locus_tag="PLY56_03020"
     CDS             1732..3066
                     /gene="rimO"
                     /locus_tag="PLY56_03020"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ20480.1"
                     /translation="MAKSRATRVAVINLGCPKNQVDAEVMLGLLREEGFELSSEPARA
                     DVVVVNTCGFIADAKEESVQAILDAVHLKERGKVRRVVATGCLAQRYADQLRAEIPEI
                     DAFVGIGGQHRIVEAVREALAGRGYDAVGAPRAVLPEELPRVLSTAPWTAYLKIADGC
                     NRPCAFCAIPSIRGPMISRPLEAIVAEARRLAEGGVRELVLVAQDTSQYGSDLYGAPR
                     LAELLRALARIDGVEWIRPLYYYPTNVDDALIAATAEEEKVCSYMDIPLQHVARSVLR
                     RMHRPGDSDAYLKLIERIRQACPEIALRTTFLVGFPGETDAEFEELLQFVQEARFDRM
                     GAFTFSPEEGTAAFDLPDAVPASLARRRFDQLMRVQQRISLERNRQLVGQRIRVLVET
                     AAGPRGRDRVGRSYRDAPEIDGTVILRDSHATPGTFVEATVTDAKPYDLIAN"
     gene            3082..3825
                     /locus_tag="PLY56_03025"
     CDS             3082..3825
                     /locus_tag="PLY56_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOJ20481.1"
                     /translation="MTPQAAARYNGRVQCSRLAVLPTYNERENLPLIVERLLALNADL
                     DILVVDDNSPDGTGDAADALARVHPEVHVLHRANREGLGRAYVAGFSWALEREYQVIV
                     QMDADLSHNPKYIPAMLAQLKESDLVLGSRYVPGGGVEDWGLVRRWISRCGSWVARAM
                     LGIPYRDLTGGFKAWRRETLERIDMSGVRSNGYCFQIEMTFRAHQHGARITELPIIFP
                     NRRFGTSKMRGRIVWEAIIRLWQLRSERD"
     gene            3818..4720
                     /locus_tag="PLY56_03030"
     CDS             3818..4720
                     /locus_tag="PLY56_03030"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOJ20482.1"
                     /translation="MISSILHSMRPHQWVKNSFLFAGALFGRYAGAPVGAADLFYVTL
                     AFLRFCFLSGAVYLVNDVVDRERDREHPIKRNRPIASGRLPVPTALLAALLCVVVAGA
                     LAATISLRPPADGGHRYFWITSLLYLVLNVGYSLIAKDLVILDLLFIALGFVVRVVAG
                     CVAIPVSISPWILICTLLLALFLGLCKRRHEMILLGEGNGATRPVLSHYSPELLNQMI
                     AETAAATIVAYMLYTFQPHGNAPSDGRMMLTVPFVIYGVWRYQYLAYRQDLGGSPELA
                     FRDPLFLANGLLWVGTVLALAFLR"
     gene            4775..5158
                     /locus_tag="PLY56_03035"
     CDS             4775..5158
                     /locus_tag="PLY56_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ20483.1"
                     /translation="MTKPDTLALCLILASVLLGSVGQIVLKGGMENAPPVTSGWALAS
                     AFRSPAVLAGLACYVVATLAWLVVLQRVPVSFAYPMISLNYIFVTLLAKYVHGEAVPS
                     LRYLGLALILAGVAVIARTPAPDPK"
     gene            5172..5618
                     /locus_tag="PLY56_03040"
     CDS             5172..5618
                     /locus_tag="PLY56_03040"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOJ20484.1"
                     /translation="MPHFETSIVVNAPVAEVFALARDIERYPEFMPDVKSIRVTERSG
                     KSQVSEWVALVSQFRIEMKWSEEDIWDEETYTCTWRQIKGDFQQYDGRWTFEPAPEGG
                     TRMTMQVDYRYEVPLIGPMLKALVHKLMVQNANDILAALKKQAEGG"
     gene            complement(6070..6321)
                     /locus_tag="PLY56_03045"
     CDS             complement(6070..6321)
                     /locus_tag="PLY56_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20485.1"
                     /translation="MRNANVCRFCGREILTNPYLGDVNPEHVRAIRDPDAPSVEILHA
                     QDRVYDTFCSKEHYIRWLKAERHRLPDPPPHTGGPCPGE"
     gene            complement(6335..7000)
                     /locus_tag="PLY56_03050"
     CDS             complement(6335..7000)
                     /locus_tag="PLY56_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ20486.1"
                     /translation="MKGATAGGIEMPNVRGIDISGGSDALREIRIATDEGEVWGWMHP
                     APGATLGVVMVGGAGGGVTGPASIYPELAEAMRLHGAVSLRLDYRHATDLEECVYDTL
                     VGTEALGQMGVERVALIGWSFGGAVVITAGALSDRVVGVATIASQSYGTELAPALSPR
                     ALLLLHGTGDQTLPYACSEEIYRRAREPKELEIYPLANHGLDEVRDRMKDRLIEWLLR
                     VLP"
BASE COUNT         1290 a         2411 c         2338 g         1309 t
ORIGIN      
        1 ctcgccgctt ccggtggccg cggccatcgc cagcctcaag gtaatccagt cgcgtggcct
       61 ggtggagcac gcggcgaaga tgggcgagat catgatgccg gagctggcgc ggatccaggc
      121 caagtatcct ggcccgctcg gatgcctgac gggcaagggc ctggtggccg gcattcaggt
      181 ggtgaagccg ggtaccaagg agcctgatcc ggacacggcg ttggcgatca acatcgcgtg
      241 catgcaccgg ggtgtgctca tgttcgcccc ggtgggcggc gccggcgagt gcatcaagat
      301 cgccccgccg cttacgatcc ccgaggatgc gctccgcgag tcgatcagcg ccttcgagga
      361 agcggtcgac gcggtgcttg gatagtctcg ggagcatccc gctctctatc gcccccctgg
      421 tgttgcgccg gcagacggca gcccaggggg gctatcgctc ggcactcgga ttccagcttg
      481 accagtgggc ctctttcctg gtggaccgcg aagctccgcc tgttgccagc gttcccgcga
      541 aaggcagcag ccgcgggcac gctgctcggt accgctacgt ttggccaggg acgaccgaac
      601 cgggagcgcc ccggacgcgt cggtaataga agctcaaggc tgtgtggcgt catatgaacg
      661 cgccaccacg ctgcgttctg aagatggagg ctgcaatggc gaaggataag agcgcaccaa
      721 agagcgagcc aaagaagaag ccgctaaaaa cgctgaagga gaagcgtgcc gaaaagcgcg
      781 ccgaaaagag atcctctcta tcgatcccac agccatagca tcccgccccg atagcggccg
      841 gccacagtgc ccacccaggg cgctgtttgc accgctcgga cccgccgggc gggagttcag
      901 atgccaggcc gcaacgcggc ggcggaacgt tcccctatcg atatgagatg cgcccggccc
      961 gagccaggta gcttcggcgc atgatcccgg atggctgccc gcatacctgc tggatgtggg
     1021 cagcttcccg ttagcaaggt gctggctcga ggcgcctcgt ctcccctctc acctccggaa
     1081 catgaagaag accgccccca ggatgaacag gaacgcgatg acgtggttcc agcgcaggct
     1141 ctcgcgcagg taggccacag agaagcccgc gaagacgacg agcgtgatca cttcctgaat
     1201 caccttgagc tgagccgccg aaaactgtcc atgcccaatg cggttcgcgg gcacctgaaa
     1261 gcaatactcc acgaaggcga tgccccaact cgccaggatc acaatccata gtggcgacga
     1321 cttgaagcgg aggtgtccat accaggcaaa ggtcatgaat atgtttgaaa tcgtaagcag
     1381 gatgatcgta cgcatccgag cggagcattg ccccttctaa tgtgtcgaga atcgacctcg
     1441 agcgccaggg cgggcgccgt ggtctggccg agatacccca gaccggcgcg agcgggcgtc
     1501 cgatgatcag aagagacgct gtcagcacct gacagcgcct cgccattgcg cgggagaact
     1561 ccccagcggg aagattggcg atagtgagcc cagattcctt tggacccgtg ccatccctga
     1621 cgcccggccc cttccttcca accccgcgca ggcgcgggca caggagaccc cggcaccacg
     1681 ttgacggtgc gggcaggctg tgatacgatt gggagtacgt gagggagatc tatggcaaag
     1741 agtagagcga cgagggtggc ggtcatcaac ctggggtgcc cgaagaacca ggtggatgcc
     1801 gaggtgatgt tggggctgct gcgcgaagag ggcttcgagc tctcgtccga gcccgcgcgc
     1861 gcggatgtgg tggtggtgaa cacctgcggg ttcatcgccg atgcgaagga ggagtcggtc
     1921 caggccatcc tggatgccgt gcacctgaag gagaggggca aggtgcgccg ggtggtggcc
     1981 accggctgcc tggcccagcg ctacgcagat cagctccgcg ccgagatccc ggagatcgac
     2041 gccttcgtcg ggatcggcgg gcagcatcgg atcgtcgagg cggtgcgcga agcgttggcc
     2101 ggtcgcggct acgacgccgt gggcgcgccg cgggcggtgc tcccggaaga gctgccacgg
     2161 gtcctctcca cggcaccgtg gaccgcctac ctgaaaattg ccgatggctg caaccggccc
     2221 tgcgccttct gcgccattcc gagcattcgc gggccgatga tcagccggcc gctggaggcg
     2281 attgtggcgg aagcccgacg cctggccgag ggaggcgttc gcgagctggt gctcgttgcc
     2341 caggatacca gtcaatacgg atcggacttg tatggagccc cgcgcctcgc ggagctcctc
     2401 cgcgcgctgg cgcgcatcga cggagtcgag tggattcgcc cgctctacta ctaccccacg
     2461 aacgtcgacg acgccctgat tgccgccacg gccgaggaag aaaaggtctg cagctacatg
     2521 gatatccccc tgcagcacgt tgcccgatcg gtgcttcgcc ggatgcaccg gcccggggat
     2581 agcgatgcct acctgaagct gatcgagcgt atccgccagg cctgccccga gatcgccctg
     2641 cgcacgacct tcctggtcgg cttccccgga gagacggatg cggagttcga ggagctcctc
     2701 cagtttgtcc aggaggcgcg ctttgaccgc atgggcgcgt tcacattctc gcccgaggag
     2761 ggcaccgcgg cgttcgacct cccggacgcg gttccggcgt cgttagcgcg acgccgtttc
     2821 gaccagctca tgcgcgtcca gcagcggatc tcgctcgagc gcaaccggca gctggtcggc
     2881 cagcggatcc gtgtcctggt cgagaccgcg gccggcccgc gaggccggga tcgcgtgggg
     2941 cgctcctatc gcgacgcccc tgagatcgat ggcacggtca ttttgcgtga tagccatgcg
     3001 acgcccggca cgtttgtcga ggccaccgtg accgacgcga agccatacga cctcatcgcc
     3061 aactgaatgc ctgagcgcgc gttgacacct caagcggcgg cacggtataa tggccgtgtg
     3121 caatgctcca gactggccgt gcttccaacc tataacgagc gcgagaacct ccccctgatc
     3181 gtcgagcgcc ttctcgcgct gaacgcggac ctggacatcc tggtggtgga cgataactcc
     3241 cccgatggta cgggtgacgc agcagatgcg ctcgcccgcg tccatcccga ggtgcatgtg
     3301 ctccatcgcg cgaaccggga aggcctcggc cgggcctacg tcgccggctt ttcctgggct
     3361 ctggagagag agtaccaggt gattgtgcag atggacgcgg acctctcgca caacccgaag
     3421 tacatcccgg cgatgctggc ccaactcaag gagagtgacc tggtgctggg ctcgcgctac
     3481 gtgcctgggg gcggcgttga ggattggggg ctggtccgcc gctggatcag ccgctgcggc
     3541 tcctgggtgg cccgggccat gttgggcatc ccctatcgcg acctgacggg cggcttcaag
     3601 gcgtggcgtc gcgagacact cgagcgaatt gatatgagcg gcgttcgctc gaatggctac
     3661 tgcttccaga ttgagatgac cttccgtgcc caccaacacg gtgcgcgtat cacagagtta
     3721 cccatcatct tcccaaaccg acgcttcgga acatcgaaaa tgcgcgggcg gatcgtctgg
     3781 gaggccatca tccgcttgtg gcaattgagg tctgagcgtg attagcagca tcctccactc
     3841 catgcgcccg caccagtggg tgaagaactc cttcctcttc gccggggccc ttttcgggcg
     3901 atatgcgggg gctccagtcg gggcagcaga cctcttctac gtcacgctgg cttttctccg
     3961 cttctgcttc ctctccggcg ccgtctacct ggtcaacgac gtcgtggacc gcgagcgtga
     4021 ccgggaacac cccatcaagc ggaatcggcc gattgccagt ggcaggctgc cagtcccaac
     4081 ggcgctgctg gccgcccttc tctgcgtggt ggtggccggc gcgcttgccg ccaccatctc
     4141 cctccgcccg ccggccgatg gtgggcatcg ctatttctgg atcaccagcc tgctctacct
     4201 ggtgctgaat gtcggttact cgctcatcgc caaagacctc gtcatcctcg atctgctctt
     4261 catcgccctt ggcttcgtgg tgcgcgtcgt cgctgggtgc gtggcgattc cggtgagcat
     4321 ctcgccctgg atcctgatct gcacgcttct gctggcgctc ttcctcgggc tttgcaagcg
     4381 ccggcacgag atgatcctcc tcggtgaggg caacggcgcc actcgcccgg tgctgtcaca
     4441 ctatagccct gagctcctga accagatgat cgccgagaca gccgcggcca ccatcgttgc
     4501 ctacatgctc tatacctttc agccacacgg gaatgctccc tctgatggtc gaatgatgct
     4561 cacagtgccc ttcgtcatct atggcgtgtg gcgctaccaa tacctcgcgt atcgccaaga
     4621 tctcggcggc agcccagagc tggcgttccg cgacccgctc ttcctggcca acggcctgct
     4681 ctgggtcgga accgtgctgg ctctcgcgtt tctgcgatag cgcgaggcgc atcgagaggg
     4741 ctgaatgcgg aagcttcgct ttccaggagg aaagatgacc aagccggaca cgcttgccct
     4801 gtgcctgatc ctggcctcgg tgttacttgg gtcggtgggc caaattgtgc tgaagggcgg
     4861 gatggagaac gcgccaccgg tgacatcggg ttgggcgctc gccagtgcgt tccggagtcc
     4921 cgccgtcctg gcgggcctgg catgctacgt cgtggcaacc ctcgcgtggc tcgtcgtttt
     4981 gcagcgcgtg cccgtcagct tcgcctaccc gatgatcagc ctgaactaca tcttcgtgac
     5041 gctcctggcc aagtatgtgc atggagaggc agtgccctcg ttgcgctacc ttggattggc
     5101 gctcatcctg gccggcgtcg ccgtgatcgc gcgaacgccc gcgccggatc cgaaatgaga
     5161 ggaagagaag catgccccat tttgaaacca gcatcgttgt caacgctcct gtcgcggagg
     5221 tcttcgcgct cgctcgtgac atcgagcgct accccgagtt catgccggat gtgaagtcga
     5281 tccgtgtgac ggagcgcagt ggcaagagcc aggtgagcga gtgggtcgcg ctcgtctccc
     5341 agttccgcat cgagatgaag tggtccgagg aagatatctg ggacgaggag acgtacacct
     5401 gcacatggcg gcagatcaag ggggatttcc agcaatacga tggccggtgg accttcgagc
     5461 cggcgccgga gggcggcacg aggatgacga tgcaggtgga ctaccgctat gaggtgcccc
     5521 tcatcggccc catgttgaag gctctggtgc acaagctgat ggtccagaac gccaacgaca
     5581 tcctcgccgc gctgaagaag caggctgaag ggggatagtc ggaccatccc ccgcgggcca
     5641 gcctgggctc ccccacccgt cattccctgg agctgcggca cggccctcgc tggcccgacc
     5701 gcggcgcgcc cgtgcccggc tcgggccaac tccaccctca atggagcgtg ctcagcgcca
     5761 ggcagaagca ggaattgcgc gggcaagttc tgcctgtggg cgcgcgacat gccgcgacca
     5821 actctctcgc gcaccctatg gtcgccgtct cgtcctatct gaagaacacg caggtgttga
     5881 cctgcccgcc gagaacgccc ggcgacgtca acggggaatt aggggctttc tgatcgcggg
     5941 gccggtgacc aacggctatg cattcagcta ttacatctcc ggcagcaaca cggcgcgcgc
     6001 tccgatttcc gaggcgcagg tagacaaacc gtcggttggg cgcccgagag agcgccctcg
     6061 cgccggcatt cactcgcccg ggcaaggccc gccagtatgc ggcgggggat ccggcagccg
     6121 gtggcgttcc gccttcagcc agcgaatgta gtgctctttg ctgcaaaagg tatcgtaaac
     6181 gcggtcctgc gcatgcagaa tctcgactga aggcgcatcc gggtcccgaa tggcccgcac
     6241 atgctccggg ttgacatcgc ccagataggg gttcgtcagg atctcgcgcc cgcagaaacg
     6301 gcagacgttc gcgttacgca taggtctcct ccgactacgg caggacgcgg agcagccact
     6361 ctatcagccg gtccttcatc cggtcgcgca cttcatcgag gccgtgattt gccagggggt
     6421 agatctccag ctcctttggc tcgcgggcgc gccggtagat ctcctcggag caagcgtagg
     6481 gcagcgtctg gtcgcccgtg ccatgcagaa gcaggagcgc gcggggagag agcgctggcg
     6541 cgagttccgt gccgtagctt tgactggcga tagtcgccac gccgacaacg cggtcgctga
     6601 gcgctccggc ggtgatcacc accgcccctc caaaggacca accgatcagc gcgacgcgct
     6661 ccactcccat ctgcccgagg gcttccgtgc cgaccagggt atcgtagacg cactcctcca
     6721 gatcggtggc gtgacggtag tcgaggcgaa gcgataccgc gccatgcagc ctcatcgcct
     6781 cggcaagttc cgggtagatg ctggccggcc cggtcacccc tcccccggca ccgccgacca
     6841 tcaccacgcc cagagtcgcc cctggcgccg gatgcatcca cccccacact tccccttcat
     6901 ccgtcgcgat ccgaatctcg cggagagcat cgctcccacc gcttatgtcg atgcccctga
     6961 cattcggcat ctcgatcccg cccgctgtgg cgcctttcat ccaagcgcac tccctccacc
     7021 caaagcgtgg ccgctttgcc gcggtctccg tccaccactg aagcccaggc tctgcagcct
     7081 ccccggaatg gcagcagtca gcaccccgtc tcatgatacc ccctggattt tcccgaccat
     7141 ccctcgcagt aaacagacag cctggccgca atccagatcg cgcctctttt caggtctccg
     7201 cgaccctggc caggagacca ggaagcagca ccctgcccaa agccatcgcg ggtcgcgctc
     7261 gctgcatcct gtttcgaagc agggaactgt ccggtcgccg tcgaattgtt cttcttagag
     7321 ggatggtgga tggtagacag cagggatg
//
LOCUS       DAOSCV010000074         7281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6563_length_7281_cov_3.796291, whole genome shotgun sequence.
ACCESSION   DAOSCV010000074 DAOSCV010000000
VERSION     DAOSCV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6563_length_7281_cov_3.796291"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLY56_03055"
     CDS             complement(<1..868)
                     /locus_tag="PLY56_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ20487.1"
                     /translation="MFPSIFTDELRVDITEGLPIIKSWGLHAVDLRNMVFGKPAEALP
                     PEELPRLRKLLDDHGMKVGCLESSLAKVHLPGPERCKAEEEKLEGIIRAADALDCRLV
                     RAFFYWQPPAAESGLLAHRSDLQQQVLDRFAPLAERARQAGLTLAFENCGVTPDEVFA
                     ILDLLGVPRWGLAWDCHNSWDCDERRRDEDAYILRMVQRAKLVHVKATQAVPGTAEEL
                     IPYAKVLQACNNAGVAGPVSAETHNPDPSVSHVEMSRRTVEVIQKAWPSAAPGSLFHE
                     RRSTEGIARPWAD"
     gene            complement(1000..2772)
                     /locus_tag="PLY56_03060"
     CDS             complement(1000..2772)
                     /locus_tag="PLY56_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20488.1"
                     /translation="MPAGNCSVLVVLDSRRIAERASADRTVFAALDHFRPAWEALECA
                     CQYALPPGHVSPRAAYILAHDGAGAGLPARAATEIAEAVRAGAGLISFDREVEKWPEC
                     LRALVPAPAGVTTVEQLRFPAPPGFITHGHAEGETLSLFAPLQVVTFEAAEGFQSLVQ
                     TLDGRCVIASAQVGAGRVVVFGTGEQIYADGILGHTRGIDGLMWRSLIWAAAKPFPMR
                     CIPPYIAARMDDCHGTHTAFRYVDVLNRHGIGPNLGLFIDEMGPTDWAAARRLFEKGG
                     ADFSMHAFRDDFYKASPKWRPYGVLPDKPDLSDGGKHTAFEGLSVDHQTGRDLDDETV
                     RRNFKRMDDAFARAGIKHSRVLNSHFGEIGWRAVPLFLERGVEFPCNNSVVGQLYGNQ
                     PPWRPKPYGVRGPNGRHGLVLDRCPRHPGFTFIGSAPSHLGGTYMITDILYGHTPFYG
                     ESDRSRPDDSIARGIANLKLELDNLAFGEIFCHEERIDCISLEEWEYIIEGIIRGISD
                     CDWIPAEREQVSVTCKRLIDSGLVYADVTESGLHCEFSGVTDGPSPLTLWVNEGDSCR
                     RLMTDIPQLEGFLALDLPESIYAS"
     gene            3118..3744
                     /locus_tag="PLY56_03065"
     CDS             3118..3744
                     /locus_tag="PLY56_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ20489.1"
                     /translation="MKVECYVLGPLANNVYLVVDEETQDAAILDPGIESEPALDHIRE
                     GGYNLRYVINTHGHIDHVYCDAFFMAETGASLLIHADDLPVLEAADEQAAWLGCEPPV
                     RPVPTRLLQDGETIEIGKLSFTVLHTPGHTPGGICLLGDGVLFSGDTLFAGSIGRTDL
                     PGGDYGVLLRSLRERILTLPDETVVCPGHGQRTTIGRERQMNPFLVGI"
     gene            3757..4626
                     /locus_tag="PLY56_03070"
     CDS             3757..4626
                     /locus_tag="PLY56_03070"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HOJ20490.1"
                     /translation="MRLGAHMRIAGGFGTAVREAVEAGCEALQIFTKSPQQWARREVT
                     EAEGQEFQRQCAEAGIYPVIAHDSYLINMASADEALRRKSVEGFISEVWSCHMLGVKA
                     LVTHMGSAGKEPMDEALERLCASLDEVLAATADSGVDVVLETTAGQGNSLGSRFEEFP
                     RIFDRVKDDSRLGVCLDTCHIFVAGYDIRTREGYEEVMAEFDRLVGIHRLRAIHLNDA
                     KKALGSRVDRHEQIGQGMLGLDAFRWVVNDPRLQHLPGILETPKLDAEKREMDPVNLA
                     LLRSLMTGPTCPT"
     gene            4614..5210
                     /locus_tag="PLY56_03075"
     CDS             4614..5210
                     /locus_tag="PLY56_03075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ20491.1"
                     /translation="MPYLVGLTGPYTDQAFEVKDGRTTIGRGPACNLILAQDAAVSRA
                     HAILVVSGSVVQVQDAGSLHGVWVNGKRTAQAHLRLGDCVQVGASVFQLLPVPAPPDP
                     PIRLKVILPPATGAPGARGAPDRPLPLAVGCLYYLAALLFPIPFGFIAAFTCRAKPHP
                     ADRQLGNTCLIVSILSTMLQIGISVLLVQSLLSRSYFF"
     gene            complement(5255..6271)
                     /locus_tag="PLY56_03080"
     CDS             complement(5255..6271)
                     /locus_tag="PLY56_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20492.1"
                     /translation="MLSRMLALPLIGVILLAVFFLAARLVLLQKERTAIEEPIQAAKT
                     APAAQEASRLGHTSCQEIEERQSEERARQVSVLDQKQFTAAMQSSQDPAAWNAPEMVA
                     AVYMKLLPESERRRRLAQLGVGPEWSEARIEALDIHAGTIRLRLGNRGILLTGDLERK
                     KFRKALRAQFPEASLLLVRAVEGIFPNAPGLASGGDYQYKGDYTCKFDLSTLPKNVDV
                     RCVCFTGNVGWGHSRVTGQWDASVRGAWMEFPFGIYEEIRFNPPVMEHSELPPGLMRT
                     SSEDCTDEMCLWAEANAGNRAANPGGGQWGVIGEYTEWFAAGGVYEMRIEYELCWRLK
                     QRGD"
     gene            complement(6429..>7281)
                     /locus_tag="PLY56_03085"
     CDS             complement(6429..>7281)
                     /locus_tag="PLY56_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20493.1"
                     /translation="EAFAAVYMKMLPHDERRRRLARLGAGPKWSEARIEAVDIYNAAI
                     RLQLGTRRRILAAREGDKQFRRALRREFPEVAALIVRSAREELTNQGGGVQADYLAGG
                     SYSCVFDLSSLPRTIDVRRVEFVGHIILGPTWKTQVYDATVREVWMTFPFSGLKKLTF
                     DRHSNHPVSVVGWGRGGVRLGRGERRQPRGQPGWRLVGDHRRAKRVERRVLRAMAHPR
                     LRLLVIPVRARRAASSRLNREDRPRPPALACRPPPRRERPPRLRRGFSASPGSIAPTS
                     CLHRYRR"
BASE COUNT         1255 a         2299 c         2437 g         1290 t
ORIGIN      
        1 tatccgccca ggggcgcgcg atcccctcgg ttgagcggcg ctcgtggaaa aggctgccgg
       61 gagcggcgct cggccaggcc ttctggatca cctccaccgt gcgccgcgac atctcgacgt
      121 ggctcaccga gggatcgggg ttgtgggtct cggcgctcac cgggccggcc acgccggcgt
      181 tgttgcatgc ctgcagcacc ttggcatagg ggatgagctc ctccgccgtg cccggcaccg
      241 cctgcgtcgc cttcacgtgg accagcttgg cgcgctgcac catgcggagg atgtaggcgt
      301 cctcatcgcg ccggcgctcg tcgcaatccc agctgttgtg gcagtcccac gccaggcccc
      361 acctgggcac gcccaggagg tccagaatgg caaagacctc atccggggtg acgccgcagt
      421 tctcaaaggc gagggtgagc ccagcctgcc gggcgcgttc ggcaaggggc gcgaagcggt
      481 ccagcacctg ctgctgcagg tcggagcggt gcgcgagaag cccggattcg gccgccgggg
      541 gctgccagta gaagaaggcg cgcaccagcc ggcagtcgag ggcatccgcc gcgcggatga
      601 ttccctccag cttttcctct tccgctttgc atcgctcagg acccggcagg tgcaccttgg
      661 ccagcgagga ctccaggcag cccaccttca tcccgtggtc atcgagcagc ttgcgcagcc
      721 gcggcagctc ctccgggggc agtgcctcgg ccggcttgcc aaacaccatg ttgcgcaggt
      781 ccaccgcgtg gaggccccac gacttgatga tcggcaggcc ctcagtgata tcgacgcgta
      841 gctcgtcggt aaagattgac ggaaacatgc tctctccccg tggctttgat aacagccctt
      901 atcacgcgga atgggtgagc gaagaggcgg aaacagacca gccgtggaga ggcttcgcac
      961 accctttccg caatccggcg atttactggc cggagacagt tagctcgcgt agatcgattc
     1021 gggcaggtca agggcgagga agccctcgag ctgcgggata tcggtcatca ggcggcggca
     1081 ggagtcgccc tcgttcaccc agagggtgag tggcgaaggt ccgtccgtca cgccggagaa
     1141 ttcacagtgc agccctgatt cggtaacgtc ggcatacacc aggccggagt cgatgaggcg
     1201 cttgcaggtg acgctgacct gctcgcgttc ggccggtatc cagtcgcaat cggagatccc
     1261 gcggatgatc ccctcgatga tgtactccca ctcctccagg ctgatgcagt cgatccgctc
     1321 ctcgtggcaa aagatctcgc cgaaggcgag gttgtcgagc tccagcttca ggttggctat
     1381 accgcgggca atggagtcat cgggccgcga gcggtcggac tcgccgtaga acggagtgtg
     1441 cccgtagaga atatcggtga tcatgtaggt gccgccgagg tgcgaaggcg ccgagccgat
     1501 gaaggtgaag ccagggtgtc gggggcagcg gtcgaggaca aggccatgac gcccgttggg
     1561 accgcggacg ccgtagggct tgggccgcca cggaggctga ttgccataga gctgaccgac
     1621 gacgctgttg ttgcagggga actccacgcc gcgttccagg aagagaggca ccgcgcgcca
     1681 gccgatctct ccgaagtgcg agttcagcac gcggctgtgc ttgattccgg cgcgcgcgaa
     1741 ggcgtcatcc atccgcttga agttgcggcg caccgtctcg tcatccaggt cacgcccggt
     1801 ctggtgatcc accgaaagac cctcgaaggc ggtgtgcttg ccgccgtcgg agaggtcggg
     1861 cttatcgggg agcacgccgt agggccgcca cttggggctc gccttgtaga aatcgtcgcg
     1921 gaaggcgtgc atggagaagt cggcgccacc cttctcgaag aggcgccggg cggcagccca
     1981 atcggtgggc cccatctcat cgataaacaa cccgaggttt ggaccgatac cgtggcggtt
     2041 caggacatca acatagcgga acgcggtgtg cgtgccgtgg cagtcatcca tgcgcgcggc
     2101 gatgtagggc gggatgcatc gcattgggaa gggtttggcc gcggcccaga tcagggagcg
     2161 ccacatcagg ccgtcaatcc cgcgcgtgtg cccgagaatc ccatccgcat agatctgctc
     2221 gcccgtgccg aagacgacga cgcgcccggc gccgacctgg gcgctggcga tcacgcagcg
     2281 accgtccagc gtctgcacga gcgactggaa gccctcggcc gcctcgaagg tgaccacctg
     2341 cagcggggca aagagcgaga gcgtctcgcc ctctgcgtgc ccgtgggtga tgaagccggg
     2401 gggcgccggg aaacgcagct gctcgaccgt ggtcacgccc gcgggcgcag gcacgagcgc
     2461 gcgcaggcac tccggccatt tttccacctc gcggtcgaag ctgatgaggc ccgcgccagc
     2521 tctcactgcc tcggcgatct ctgtggcggc gcgcgccggc aatccggccc cggcgccatc
     2581 gtgcgccagg atgtaggcag cccgcggcga gacgtggccg gggggaagag cgtattggca
     2641 ggcgcactcc aacgcttccc aggcggggcg gaagtgatcg agggcggcga acacggtccg
     2701 gtcggcgctc gcgcgctcgg ctattcgccg gctatcgaga actaccagaa cgctacagtt
     2761 tcctgctggc atgggttgtc cctcctccaa tgacggcgaa tccgcgcgat ggtgccccct
     2821 tcagacagcg cccggctgag ccgacaggcg cggatccggc gcgtctgcat gactctcttg
     2881 ggcactgcaa cgcgagatcc tgccctggaa cgagaggaga gcgcatgcgg gagcgcagag
     2941 cggcgggcgg atcgcacgtt cttgcccatc acctaagcga tccaccgatg cgattctggg
     3001 agttggcgtc tacatcaggg taagccatag gcccgggatc agggcgggta acggctctgc
     3061 cagggatccg cggcgggcgc ggcgaacgta gcggtaatcc tgtcgctgga ggcgcagatg
     3121 aaggtagagt gctatgtgct cggacccctg gcgaataatg tgtacctggt ggtggatgag
     3181 gagacccagg atgccgcgat cctcgatccg ggaatcgaga gtgagccggc gctggatcat
     3241 atccgcgagg ggggatacaa tctccgctat gtgatcaaca cgcacgggca cattgaccac
     3301 gtatactgcg atgccttctt catggcggag accggggcgt cccttctgat ccatgccgat
     3361 gacctgccgg tcctcgaggc cgcggacgaa caggcggcgt ggctgggatg cgagccgccg
     3421 gtgcggccag tgccgacgcg cctgttgcag gatggagaga ctatcgagat cggcaagctc
     3481 agtttcacgg tgctgcacac accggggcat acgcctggag ggatctgcct gctcggggac
     3541 ggcgtccttt tcagtggaga cacgctcttt gccggctcca tcgggcgtac cgacctgccg
     3601 gggggagact atggggtgct cttgcgctcg ctgcgggagc gcatcctgac cctgcctgat
     3661 gagacagtgg tctgcccggg gcatgggcag cgaacgacca tcggccggga gcggcagatg
     3721 aaccccttcc tggtggggat atagaggaga gatggaatga ggctgggggc acacatgcgc
     3781 attgcgggag gcttcggcac ggcggttcgc gaagccgtgg aagccggttg cgaggcgctc
     3841 cagatcttca ccaagagccc gcagcagtgg gcgcgacgag aggtcaccga agcggagggc
     3901 caggagttcc agcgccagtg cgccgaggcg gggatttacc cggtcatcgc ccacgactcc
     3961 tatttgatca acatggcgtc tgccgatgag gcgttgcgcc gcaagtcggt ggagggcttc
     4021 atcagcgagg tctggagctg ccacatgctt ggggtgaagg ctctggtgac gcatatgggg
     4081 agcgccggca aggagccgat ggacgaagcc ctggagcgcc tgtgcgccag cctggacgag
     4141 gtgctcgccg cgaccgccga ttcgggggtg gatgtcgttc tcgagacgac cgccggccag
     4201 ggaaacagcc ttgggagccg tttcgaggag tttccgcgaa tctttgatcg ggtgaaggat
     4261 gattcgcggc tgggcgtttg cctggatacc tgccacatct tcgtggccgg ctacgatatc
     4321 cgcacgcgcg agggctacga ggaggtgatg gcggagttcg accgcctagt cgggatccat
     4381 cgcctgcgcg cgatccacct gaacgatgcc aagaaggcgc tgggaagccg ggtcgaccgg
     4441 cacgagcaga tcggccaggg tatgctcggc ctggatgcgt ttcggtgggt ggtgaacgac
     4501 ccgcgcctgc aacatctgcc tggcatcctg gagacgccga agctagatgc cgagaagcgg
     4561 gagatggatc cggtaaacct agctctcctt cgctcgctga tgacggggcc aacatgccct
     4621 acttagtcgg actgacgggg ccctacaccg accaggcctt cgaggtgaag gatggacgca
     4681 cgaccatcgg ccggggccca gcatgcaatc tgattctcgc ccaggatgcc gccgtctcgc
     4741 gcgcgcacgc gattctggtg gtgtctggga gcgtggtgca ggtacaggat gccgggagct
     4801 tgcatggcgt gtgggtgaac ggcaagcgaa cggcgcaggc gcacctgcgc ctgggcgact
     4861 gcgttcaggt gggtgcgagc gttttccagc tcttgccggt gcccgcgcca cccgacccac
     4921 cgatccgtct gaaggtgatc ctgccaccgg ccaccggcgc gccaggcgct cgtggcgctc
     4981 cggatcggcc gctgcccctg gcggtggggt gcctgtacta cctggcggcg ttgctctttc
     5041 ccatcccctt tggctttata gccgcgttca cctgtcgcgc aaagccacat ccggcggacc
     5101 gccagctggg caacacctgc ctgatcgtct ccatcctctc cacgatgctc cagattggga
     5161 ttagcgtcct cctcgtccag agtctcctgt cccgctccta cttcttctga ggcgagcgcg
     5221 ggtgagcacc ggccccgcag gcatcgcgag gagatcaatc cccgcgctgc ttcagccgcc
     5281 agcacaactc gtattcgatt ctcatctcat acacgccacc ggcggcaaac cactcggtgt
     5341 attctccgat cacgccccac tggccacctc caggattggc cgcgcgattt ccggcgttcg
     5401 cttcggccca caggcacatt tcgtccgtgc aatcctcgga ggaggttctc atgaggcccg
     5461 gggggagctc gctgtgctcc atcaccggcg ggttgaatcg gatctcctcg tagatgccaa
     5521 acgggaactc catccacgct cctctgacag aggcgtccca ttggcctgtg actcgtgaat
     5581 gcccccatcc aacgtttccc gtgaaacaga cacaacggac gtcgacgttt ttcgggagtg
     5641 tggacaagtc gaatttgcag gtgtaatctc ccttatattg gtaatcgcca ccggaggcaa
     5701 gacccggcgc atttgggaag attccctcga cggctcgaac aagcaggagc gaagcctctg
     5761 ggaactgggc acgaagagcc ttccggaact tctttctttc caggtcgccc gtcaggagga
     5821 tgccgcgatt gcctagccgc aagcgaatcg tgccggcgtg gatgtcgagc gcctcgatcc
     5881 gggcctcgct ccactctgga ccgacgccca gttgtgccag gcgccggcgg cgctcgcttt
     5941 cgggaagcag cttcatgtaa accgctgcca ccatctcggg cgcgttccac gctgccgggt
     6001 cttgcgatga ctgcatggcg gcggtgaact gcttttggtc cagcacgctt acctggcgtg
     6061 ctctctcctc gctctggcgt tcctctattt cctggcaact ggtgtgccca agccgcgacg
     6121 cctcctgtgc tgccggggct gtcttggccg cctgaatcgg ctcctcgatt gccgttctct
     6181 ccttctgcaa tagcacgagc ctggcggcga ggaagaaaac cgccagcagt atgactccga
     6241 tcaaggggag tgcgagcatt cttgacagca tcttctgcac ctggtacacg caagcacccg
     6301 cacgggttgt atcgtgcggg tgcgaatgtc cacatcgtgt ttttcgagag agtgtcgcgc
     6361 gggccatcac gatcctgcat ggatcgcaaa cgaagaatga gcgcgcgttc catccgcgtt
     6421 catctcgttt atctgcggta tcgatgcagg caggaagtgg gcgcgattga ccctgggctg
     6481 gccgagaacc cccggcggag ccgcgggggg cgctcgcgcc ggggcggtgg ccgacacgcg
     6541 agcgcgggcg gcctgggtcg atcctcacgg ttgaggcggc tgctggcagc gcgcctggca
     6601 cgcacgggga tcaccagcag tcgtaatcga ggatgagcca tagcacgcag caccctccgt
     6661 tccactctct tagctcgccg gtgatccccc acgagccgcc acccgggctg gccgcggggt
     6721 tgccggcgtt ctcctcgacc caggcgcacg ccgcctcgcc cccatcccac gacagatacg
     6781 ggatggttgg aatgtcggtc gaacgtcagc ttcttgagcc cgctaaaggg gaaagtcatc
     6841 cagacttccc ggacggtggc gtcgtatacc tgggtcttcc aggttgggcc gagtatgatg
     6901 tgcccaacaa actcgacgcg gcgcacgtcg atcgtgcgcg ggagtgatga caggtcgaaa
     6961 acgcagctgt aggatccgcc ggcgagatag tcggcctgaa ccccgccgcc ttgattggtg
     7021 agttcctctc gagcggatcg gacaatcagc gccgctactt ccgggaactc ccgccggagc
     7081 gcccggcgaa actgcttatc tccctctctg gcggccagga tgcgccggcg cgtgcccagc
     7141 tgaaggcgga tggcggcgtt gtagatgtcc accgcctcga ttcgggcctc gctccacttc
     7201 gggccggcac ccagccgtgc gagccgccgg cgccgctcat catgaggaag catcttcata
     7261 taaactgctg cgaacgcctc g
//
LOCUS       DAOSCV010000075         7261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6588_length_7261_cov_2.939634, whole genome shotgun sequence.
ACCESSION   DAOSCV010000075 DAOSCV010000000
VERSION     DAOSCV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6588_length_7261_cov_2.939634"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLY56_03090"
     CDS             <1..553
                     /locus_tag="PLY56_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009853265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOJ20494.1"
                     /translation="DREKLQERLAKLAGGVAVVQVGAATETELKEKKARIEDALSATR
                     AAVEEGIVPGGGVTYLNVIPAISELASKIDGDLRVGANIVLKALEEPARMIAYNAGQE
                     GSVIVERVKGMDRGWGYNAATGEFVNMVQSGIVDPAKVTRSALENAASIASMLLTTEA
                     VIAEEPEKPKPAPAGPPGGMGGF"
     gene            849..2087
                     /locus_tag="PLY56_03095"
     CDS             849..2087
                     /locus_tag="PLY56_03095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20495.1"
                     /translation="MQLKPPFRAYDPERDREATHRIWREVGWLKKGREEAMDRYVRAS
                     RALVAEIDGTAECLVLSTPGSLRYLDEDLPFACVIGVTTSYIARRQKLAGRLAARLVA
                     ADALDGALVTALGMFEQGFYNKLGFGTGTYEIFYAFDPAHLRVPVSPRVPCRITPDDW
                     EEVHAARLARRRRHGACNLTHPEMTRMDMDPENCFGLGYRDPRTGALGHHIWCRADNA
                     EHGPYCVVWMAYRTLEEFLELMALIQSLGDQVRLVRLCDPPGIQLQDLLDRPFQRRQT
                     SKGGEFESNAWASAWWQARILDLPGCLARTRLPWCDLRFNLSLHDPIEQLLDEDAPWR
                     GVGGEYVVTLGPESSARRGHDPSLPVLQASVNAFTRMWLGVVPATGLATTDDLSGPPE
                     LLEALEAAFRLPLPRPDWPF"
     gene            complement(2163..4961)
                     /locus_tag="PLY56_03100"
     CDS             complement(2163..4961)
                     /locus_tag="PLY56_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ20496.1"
                     /translation="MKVLSLARFVLLVLLFGPLLPAPANAQPTLLIPNAGFEDGDNDL
                     PGWSFNPGEGGEGDWSWETRRVHSGKRSLRLRKSNVAGYSMLVSDYLPVEAGKEYEVR
                     AWVQVEGRPRANVYFMVSQYTADSPEMCLPNAFSTPRPLYTGDGWKQLRVRFPVRPGS
                     ARIRIHALISQAPAAVLWDDFELGPATEEVYAPRYEKPRPEELPPLEKARERLQHRKR
                     AVAEGKVIDGRARLFFDGKPTEPAFYVAPFHNQWNAQIADFRDAGVRVYLLPLTLGRG
                     VYSDRGPWIGAGKMDFSEVDELLWRILRVDPDGYILFYLATDPYREWGEEHPTEVCTD
                     QDGRKAVVQMHPKDWGREPDPKARERYAPSLVSSVLRRDTIEAIRGLIRHVNASLPGK
                     AVAGYHIAGLNDGQFFQWARHNPKDLHLADYSEASRKAFRDWLRRFYGNDLAAFRRAW
                     DRQDLTFETAEIPSGERRLAPGFFLMLPRDQDIADYNRFYSEGTVETIHAYARVVKEE
                     TRGQALVSTYWEDACANVDSHLATGRMLQSPDIDFLAGPAAYGVRMAGEVGQCHSTWG
                     SVGLHGRIKLTEQDWRSWLSEWRDPYYDYSVGRAENAADHNAMVRRECGMMLAYGHGT
                     WWYDMSGGWFRDDQIMRGIAEARAAFSRDLKLSGRPRADVAVFVDERSFDFLRYRAAG
                     PFRYEGIVRQLYQLNQSGVPYHLYLQLDLCNKSLPEYRLYLFLNAHHLSPAEKAAIKA
                     LRRAGKTLAFVHAPGVVGEADQAAAITEITGIRVRSAGGETRLTANVAPTAGPLNPGD
                     AISSAALMGPAFVVQDEKARAVGTYPEGGTAVAARDFGTWKSLFFGPIGMTDSFLNAL
                     ARWAGAWVAAPAGDAVYASQYFLTIHALVDGEKKLTLATPSRVEDLTTGKIVSERTQN
                     LSFPMRRGETRWFALTPR"
     gene            5167..5859
                     /locus_tag="PLY56_03105"
     CDS             5167..5859
                     /locus_tag="PLY56_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20497.1"
                     /translation="MTLADWGTVALIVIALVNLVLLGGLVFLVFMVRNQLRQLRQQAQ
                     PVFNEASATLRMVKDVTSHVGQRAEAISERAEHLAADFTQRAEAISARAEMLANDMSQ
                     RAERISEEFTQRAERITSDVTRRFDEVSRDLTQTMERILDLGEDLAERVAARVDTTTS
                     VVEEAVARPLVTLASVRDGIHKGLEMWREYRSRAEGDGKPTVPTPEQEREVRRMIEET
                     PTIVPQSETVEG"
     gene            6052..6465
                     /locus_tag="PLY56_03110"
     CDS             6052..6465
                     /locus_tag="PLY56_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ20498.1"
                     /translation="MRHSELRLLEAVPGPYGEAAWVEIRSSRHRFVVLSLLVAVLLLV
                     LFAIGLHRRSRTTTLLVPVTVERGDTLWSLARNHCGSHEYLPRAVHAIRRANGLSNSR
                     LVPGQKLMIPVEVPVAEPPSAPIMQAKGPQPSAHP"
     gene            6669..>7261
                     /locus_tag="PLY56_03115"
     CDS             6669..>7261
                     /locus_tag="PLY56_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOJ20499.1"
                     /translation="MRRIRSRRLRVTWNIIKICILLLMAGTLGLLAATFYSVSKVLPT
                     HEDIDAWVPKEATLIYSSDGALLAQVYEENREPVPITEIPKQLQDATIAIEDSRFYMH
                     SGIDFRGILRALVENVRGGRVVQGGSTLTQQLARNIYLSHRRTVSRKLQEILLAREIE
                     RRLSKEEILERYLNQVYYGSGAYGVKAAARTYFGKPPS"
BASE COUNT         1268 a         2366 c         2427 g         1200 t
ORIGIN      
        1 cgaccgcgag aagctccagg agcgcctggc gaagctcgcg ggcggcgtgg ccgtggtgca
       61 ggtgggcgca gccaccgaga ccgagctcaa ggagaagaag gcccgcatcg aggacgcgct
      121 tagcgccacg cgcgcagcgg tcgaggaggg catcgtgccc ggcggcggcg tcacctacct
      181 caacgtcatc cccgcgatca gcgaactggc cagcaagatc gacggcgacc tgcgcgtggg
      241 cgcgaacatc gtgctcaagg cgcttgagga gccggcgcgc atgatcgcct acaacgcggg
      301 gcaggaaggc tccgtgattg tggagcgcgt caagggcatg gaccgcggtt ggggctacaa
      361 cgccgcgacc ggcgagttcg tgaacatggt ccagagcggc atcgttgacc cggcgaaggt
      421 gacccgctcc gcgctggaga acgcggcgtc catcgccagc atgctcctga ccaccgaggc
      481 ggtcatcgcc gaggagcccg agaagccgaa gccggcgcct gccggacctc caggaggcat
      541 gggcgggttc taagggttca aacttcgggc cgctcacggc ctgagagtgc ccagaaccgg
      601 aatccaacgc aaacgcgtcg tgaagggcac cggccactgc gccggtgcct ttcgcgctct
      661 gtccccccgc caccggcacg cattcccgat gaagcgagag gaggcaccgc tcaccgcgca
      721 agccgtggcg cgggaagaag ccagtgcaga atagggcctg ggccggcctc tctccaacag
      781 gggcaggtgc ctgggctccc tttggcgaac ccacctagcg tgcgcccacc tggcgcctgg
      841 aggaatccgt gcagctcaag ccgccatttc gagcatacga tcccgagaga gaccgcgaag
      901 ccactcaccg catctggcgg gaagtcgggt ggctgaagaa gggccgcgag gaggcgatgg
      961 accgctacgt ccgcgccagc cgcgcgttgg tggcggagat cgatggcacc gccgaatgcc
     1021 tggtcctcag cacccctggc tcgctccgct acctggacga ggatctcccc tttgcctgcg
     1081 tcatcggcgt caccacgagc tacatcgccc ggaggcagaa gctcgcgggc cggctcgccg
     1141 cgcgcctggt ggccgcggat gccctggatg gcgccctggt caccgcgctg ggcatgttcg
     1201 agcagggctt ctacaacaag ctcggctttg gcaccggcac ctacgagata ttctacgcct
     1261 tcgacccggc gcacttgcga gtgccggtat cgccgcgcgt cccctgccgg atcacgccgg
     1321 acgattggga ggaggtgcat gcagcgcgcc tggcccggag gaggcgccac ggagcgtgca
     1381 acctcaccca tcccgagatg acgcgcatgg atatggaccc cgaaaactgc ttcgggctgg
     1441 gataccgcga tccgcgcact ggcgcgctgg ggcaccacat ctggtgcaga gcggacaacg
     1501 ccgagcatgg cccctattgc gtggtctgga tggcctatcg cacgctcgag gagtttctgg
     1561 agctgatggc gcttatccag agtctgggcg accaggtccg tctggtgcgc ctgtgcgatc
     1621 cgcccgggat acagctgcag gatcttctgg accgtccttt ccagcgccgg cagaccagta
     1681 agggtgggga gttcgagagc aacgcatggg cgagcgcctg gtggcaggcg cgcattctcg
     1741 acctccccgg gtgcctggcg cgcacgcgcc tgccgtggtg cgacctccgc ttcaacctct
     1801 ctctccacga cccgatcgag cagctgctcg atgaggatgc cccctggcgg ggagttggcg
     1861 gcgaatacgt ggtcactctc ggtcccgaat ccagcgccag gcgggggcac gatccgtcgc
     1921 tcccggtgct ccaggcctcg gtgaacgcct tcacccggat gtggctcggc gtcgttccgg
     1981 ccaccggcct ggccacgacc gacgatctca gcggccctcc cgagttgctc gaggctttgg
     2041 aggccgcgtt ccgcctgcca ctcccgcgtc cggactggcc gttctaagcc ggagggccag
     2101 gcgccccctc atcgggagca tcctggccct ctcctactct ctcctggctc tcccatcccg
     2161 cgtcaccgcg gcgtgagcgc gaaccagcgc gtctcccccc ggcgcatcgg gaaggagagg
     2221 ttctgcgtgc gttcagagac gatcttgccg gtggtgaggt cttcgacgcg gctgggagtg
     2281 gccagcgtca gcttcttctc cccatcgacc agggcatgga tggtcaggaa gtactggctg
     2341 gcatagacgg catcgcccgc gggcgcggca acccaggcgc cggcccaccg agccagggcg
     2401 ttgaggaagc tatccgtcat gccaataggc ccaaagaaga gcgacttcca cgtgccgaag
     2461 tcgcgtgcag ccacagccgt cccgccttcc ggataggtgc ccaccgctcg cgccttctca
     2521 tcctggacga caaacgccgg acccatgagc gcagcgctgg agatggcatc gccgggattg
     2581 agaggccccg ccgtgggcgc cacatttgcg gtcaggcgcg tctcgccgcc cgccgaacgc
     2641 acccggatgc cggtgatttc cgtgatcgcc gccgcctgat cggcctcgcc gaccaccccg
     2701 ggggcatgca cgaaggcaag cgtcttgccc gcccggcgca gcgccttgat cgccgccttt
     2761 tcagcgggtg agaggtggtg ggcgttgagg aagagataga ggcggtactc cggtagcgac
     2821 ttgttgcaga gatccagctg caggtagagg tgatagggca cccccgactg gttgagctgg
     2881 tagagctgcc ggacgatccc ctcataccgg aagggccccg ccgcccggta gcggaggaaa
     2941 tcgaaactgc gctcgtcgac gaagacggca acgtcggcac gcggccggcc cgagagcttt
     3001 aggtcgcggg agaaggcggc gcgcgcctcg gcgatcccgc gcatgatctg gtcgtcgcgg
     3061 aaccagccgc cggacatgtc gtaccaccag gtgccatgcc cgtaggcgag catcatgccg
     3121 cactcgcggc gcaccatggc gttgtgatcg gcggcgttct ccgcccgacc cacgctgtag
     3181 tcatagtacg ggtcgcgcca ctcggaaagc cacgagcgcc aatcctgctc ggtgagcttg
     3241 atccgcccgt gcaggcccac cgatccccag gtggagtggc actggccgac ttcgcccgcc
     3301 atgcgcacgc cgtaggccgc cggccccgcc agaaagtcga tatcgggcga ttggagcatg
     3361 cgcccggtgg ccaggtggct gtccacattg gcgcacgcgt cctcccagta ggtgctcacc
     3421 agcgcctgcc cccgcgtctc ctccttgacc acgcgggcgt aggcgtgaat cgtctcaacc
     3481 gttccctcgc tgtagaagcg gttgtagtcg gcgatatcct gatcgcgagg gagcatcagg
     3541 aagaagccgg gcgctagacg gcgctcgccc gaggggatct ccgccgtttc gaaggtgaga
     3601 tcctggcggt cccacgcccg gcggaaagcg gcgagatcgt tgccgtagaa gcggcggagc
     3661 cagtcgcgga atgccttgcg cgaggcctcg ctgtaatccg ccaggtggag gtccttcggg
     3721 ttgtgccggg cccactggaa gaactggcca tcgttgaggc cggcgatgtg gtagccggcg
     3781 accgccttac ccgggaggct ggcgttcaca tgtcggatca aaccacggat cgcctcaatg
     3841 gtatcgcggc gcagcacgga agagacgaga gagggggcgt accgctcgcg cgccttggga
     3901 tccggctcgc gcccccaatc cttcgggtgc atctggacga cggccttccg gccatcctga
     3961 tcggtgcaga cctcggtcgg gtgctcctcg ccccactcgc ggtacgggtc ggtggctaga
     4021 tagaagagga tgtagccatc gggatccacg cgcagaatcc gccagaggag ctcgtccacc
     4081 tcgctgaagt ccatcttgcc agcgccgatc cagggaccac ggtcgctgta aacacctcgt
     4141 ccaagcgtga gcggcagcag gtagacgcgc actccggcgt cgcggaagtc agcgatctgg
     4201 gcattccatt ggttatggaa gggagcgacg tagaacgcgg gctccgtggg cttgccatcg
     4261 aagaagaggc gggcacgccc gtcgatcacc ttcccctccg cgacggcgcg tttccggtgc
     4321 tgcaggcgct cgcgcgcctt ttccagtggg ggcagctcct ccggccgggg cttctcgtag
     4381 cgaggcgcat agacctcctc ggtggccggc cccagctcga aatcgtccca gaggaccgcg
     4441 gctggcgcct ggcttatgag agcatggata cggatgcgcg cgctccctgg gcggacggga
     4501 aagcggacgc gaagctgctt ccagccgtcg cccgtataga gcgggcgggg cgtgctgaag
     4561 gcgttcggga ggcacatctc gggcgaatcg gcggtgtact ggctgaccat gaagtagacg
     4621 ttggcgcgcg gacgcccctc cacttgaacc caggcgcgca cctcatactc cttgcccgcc
     4681 tctaccggaa gatagtcgct caccagcatc gaataaccgg cgacgttgct cttgcgcagg
     4741 cggagagagc gcttgccgga gtggacgcgc cgcgtttccc acgaccaatc gccctcgccc
     4801 ccttcgcccg ggttaaagga ccagccgggc agatcgttat cgccgtcctc aaagccggca
     4861 ttgggaatca agagagtcgg ttgtgcattg gcgggcgccg gcaagagggg gccgaagagc
     4921 agcacgagca acacgaaccg ggcaagagag agcaccttca tccgtggaac ctccaatcac
     4981 cgcctgtggt agcggcgtat ccatcactcc ccatttcggc gcggtgcatc gcgagttcct
     5041 gcgcccgcag accgtgtgtg ttcaaggggt tccggaaccg tttccggggc gcaagcgtcg
     5101 ggtacatcct tcctgaatcg agtgacatga acgcgaaacc gcggaagcgg aagaggaggc
     5161 tgcaagatga cgcttgcgga ttggggaacg gttgcattga tcgtcatcgc gctcgtcaac
     5221 ctggtgctcc tgggcgggtt ggtcttcctt gttttcatgg tgcgcaacca gcttcgccag
     5281 ctccgccagc aggcgcagcc cgttttcaac gaggcaagcg ccacgttgag gatggtgaag
     5341 gatgtcacca gccatgtggg ccagcgcgcc gaggcgatct ccgagcgcgc agagcatctg
     5401 gcggcggatt tcacgcagcg agccgaggcg atatcggcgc gcgccgagat gctcgcgaac
     5461 gatatgtcgc agcgtgcgga gcgaatatcg gaggagttca cgcagcgtgc ggagcgaatc
     5521 acctccgatg tgacccgccg gttcgatgag gtatcgcgcg acctcaccca gacgatggag
     5581 cggattctcg atctgggcga ggatctcgca gagcgtgtcg cggctcgcgt ggataccacg
     5641 acgtctgtcg tggaagaggc ggtcgctcgg cccctggtga cgctcgccag cgtgcgcgat
     5701 ggcattcaca aggggctgga gatgtggcgt gaataccgga gccgcgcgga aggcgatgga
     5761 aagcccaccg tgcccacgcc ggagcaagag agagaggttc gccgcatgat cgaggagacg
     5821 cctacgatcg tgcctcagtc agagacggtg gaagggtgat ggtggcccgg atatcccgtc
     5881 gccgcgcgcc gctctcccgg gcgccgttgc cgcaggggca acggcgcccg cacaggcgtg
     5941 tttagaacaa gccccaggtg ggtatgtaac agcgagcaaa ccccgttatc tgccggtcat
     6001 taccgacatc ggaggccgcg cgaagctctc gacgcgtggt gaggggtgac tatgaggcac
     6061 agcgaactcc ggttgcttga ggccgttccc gggccctatg gcgaggcggc ctgggtggag
     6121 atccgctcgt cacgccaccg gtttgtcgtc ctgagcctgc tcgtggccgt cttgctcctt
     6181 gtcctctttg ccattgggct tcaccggagg tcgcgcacca cgacactgct cgtgccggtc
     6241 actgtcgagc gcggtgatac gctctggagt ctcgcgcgca atcactgcgg ttcccatgag
     6301 tacctaccga gggccgtcca tgccatccgc cgcgcgaacg gcctctccaa ctcgcggctg
     6361 gtgccgggcc agaagctgat gatcccggtg gaggtgcccg tggccgagcc accctccgct
     6421 cccatcatgc aggcgaaagg ccctcagcca agcgcgcatc cctgatccct gtctgcctgc
     6481 tcgccctggc ttttccttgc aagccccgct ccgtttcggg tataattgga gagggagctt
     6541 gggcatgaaa tgaaccacag tgcgcccgga acactctaaa ggattagagt gcggggccag
     6601 actggccctg atggcggccc gtggcgggcc ggctcaagaa ccaattcagc agggctcaag
     6661 gataaagcat gcgcagaatt cgaagtagaa ggcttcgcgt tacctggaat attatcaaga
     6721 tctgcatcct cctcctcatg gcgggaacgc tggggcttct ggctgccacc ttctacagtg
     6781 tctcgaaggt gctccccacc cacgaagata tcgatgcctg ggttcccaag gaagcgacgc
     6841 tgatctactc ctcggatggg gcgctcctcg ctcaagtgta cgaggagaac cgcgagccgg
     6901 tgccgatcac cgagatcccc aagcagttgc aggatgccac catcgccatc gaggactcgc
     6961 gcttctacat gcattccggg atcgatttcc gaggcatcct gcgcgcgttg gtggagaacg
     7021 tgcgcggcgg gcgcgtggtc cagggcggaa gcacgctgac gcagcagctc gcgcgtaaca
     7081 tttacctcag ccatcgccgg accgtgtcgc gcaaactgca agagatcctt ctggcgcgtg
     7141 agattgagcg ccggctctcg aaagaggaga tcctggagcg ctacctcaat caggtgtact
     7201 acggcagcgg cgcctacggt gtgaaagccg cagcgcgcac ctatttcggc aagccgccct
     7261 c
//
LOCUS       DAOSCV010000076         7217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6640_length_7217_cov_3.261380, whole genome shotgun sequence.
ACCESSION   DAOSCV010000076 DAOSCV010000000
VERSION     DAOSCV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6640_length_7217_cov_3.261380"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLY56_03120"
     CDS             <1..202
                     /locus_tag="PLY56_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20500.1"
                     /translation="GMPVRLGAPLDPAAATSALSGPSFATGVGLVTYAARYARYARQR
                     EGQLLEGLFAGIGRWLRGLLSR"
     gene            218..1570
                     /gene="ftsZ"
                     /locus_tag="PLY56_03125"
     CDS             218..1570
                     /gene="ftsZ"
                     /locus_tag="PLY56_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ20501.1"
                     /translation="MDSTSATPEVVNMASSTVPKQCAAIKVVGIGGGGCNAINRMIEV
                     GVQGVDFIAMNTDAMALEHSAAPTRLQLGPKLTRGLGTGGDPEKGQLAAEESRQEIVR
                     VLEGADMVFVTAGMGGGTGTGGAPVVAAIARDLKALTVGVVTKPFTNEGPRRGRAAQE
                     GLARLGERVDALIAIPNDRLRELSNRRLALRDAFLMADDILRQGVQGIAEIIYVTGDW
                     NVDFEDVRTIVQNAGTALMGIGIASGQDRATKAAQAAISSPLLETSIQGARGALVNIT
                     APDDFLIEEFDEITQLIRNATDPEEANIITGLVQGASSETGEVKVTVIATGFGSPSPR
                     PQETAEEARSWGAAMPSSPPASSGSNLRSWGFAPVSRPASPAGGNPDSPSTAPSGPAD
                     AKPPQAKSEPAQNPTPVSDLLDEVRRATREMGEGPYDPPTFLRDRKRRSGGRGSGEGR
                     "
     gene            1661..2827
                     /gene="ftsZ"
                     /locus_tag="PLY56_03130"
     CDS             1661..2827
                     /gene="ftsZ"
                     /locus_tag="PLY56_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018921335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ20502.1"
                     /translation="MTSYRDIEPFARIKVVGVGGGGSNAVNRMIQMGVQGVDFIVMNT
                     DAQALKVSQAPVRLQIGAELTGGLGTGGNPEKGQRAAEESKQEIMKALDQADMVFITA
                     GMGGGTGTGASPVVAQLCKEMGALTVGVVTKPFENEGPRRWRFAEEGLARLKEKVDTL
                     ITIPNDRLRDVAERRLPLREAFRMADDVLRQGVQGISDIIQVPGMINVDFADVQSIMS
                     NAGTALMGIGTATGENRAVQAAQGAIASPLLETSIQGAKGVLINITASPDFAMEEYDE
                     ITSLIRGVSDPDDANIITGVVFDESLQDEVRVTVVATGFGATSARSAFDQPLAATSFP
                     VTPVQPGPAAQATPASEPAPSQREATRVLEAGARALRDLGDSDIDIPTFLRPRR"
     gene            2978..4552
                     /locus_tag="PLY56_03135"
     CDS             2978..4552
                     /locus_tag="PLY56_03135"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20503.1"
                     /translation="MTSWVVKEQARARLAREIVLPGGGFRGPAGIRACILYPNTYRVG
                     MSNLGFQTIHAILNATPGLSCERAFLPDPPGRAGHGKARDGIISLETQTPLAEFDLIG
                     VSVSYEMDYPNVTRALLEAGIPPLARDRDERHPLVLAGGPCITFNPEPLAEFVDLCAI
                     GEGEEIVPGIAAVLRDVSMARGASRAELLAALAEQPGVYVPSLYEPEYTPDGSFAGPS
                     PQAGAPERIERQWVRELDRYPCASLILTPETEFGDLFCIEISRGCGRGCRYCAAGYIT
                     RPPRIRSRARLLEGVRAALPHRRKIGLVSPAISDYPDLPGLLNDLAEMGAEVSLSSLR
                     ADTLLSSPTLALAGSGESAITIAPEAGSQRLRFALNKSLTDEEIYAAVENALAAGIHK
                     VKLYLMLGLPTETEADIDAAVEMCQRIASYPGIRQVTVSANPFVPKPGTPFQWCAMQS
                     EAVLSARRKRLHTGLKTHKKLSLGGESPREAIIQGALSRGDRRLAPVLLSVAGGAELE
                     RRVPRGRNRPGRVCLP"
     gene            4784..5725
                     /locus_tag="PLY56_03140"
     CDS             4784..5725
                     /locus_tag="PLY56_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ20504.1"
                     /translation="MTTVAVFGAGGKLGRHVLRVLEKRGMAVRALCHRTPIAGENVKS
                     IPGSITDAEAVREVVRGADLVVQMATTKEDPETFFDVSVRGTFNVLEACRGARIRQFL
                     LFGGDAAMGIWFYPQPIPIDENHPLMAYPGYYAFSKVMEEVMANQYAIQYGVPVTILR
                     SSWVFEGADLLNHFSLLKNINPAEPGHGFGEVSQEILDLVSAREERIPILTDGQGVPL
                     RRHIVHVDDVMQAFDKMLDEPVAIGQSFNIAGPAPFNYRVAANYLSEKLGIPTVEIRC
                     PAYHAFEINITRARTMLGYAPENDFFRMADRAIEEAM"
     gene            5852..6193
                     /locus_tag="PLY56_03145"
     CDS             5852..6193
                     /locus_tag="PLY56_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20505.1"
                     /translation="MRMQFQHVGLTTDEKQPGEVFVPETKVWVTSPDDHPYHIEYLRY
                     EPDSPVKGPVRDLPHMAFRVSNLKEAIGDSEILLGPFEAAPGLNVAFVMKDGAVFEYM
                     EYEDESALPWK"
     gene            6201..6971
                     /locus_tag="PLY56_03150"
     CDS             6201..6971
                     /locus_tag="PLY56_03150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ20506.1"
                     /translation="MSLEGKVILVTGGTSGIGEGCSRHYAEVGAKVVIASNQEKAGRK
                     LEEALRAEGRDVRFVYTDITCEESVRNLVAQAVAAYGRIDGVHCNAGAWAPGKVTEFN
                     DSAWDLIMGVNVKGVLWTAKHVIPVMERQGKGVLLVTTSVAAHIGFPQHVIYCASKAA
                     LEAIIRCLTTDHAGVVRVVGISPGTIDTPMLAATCAGWDKPKEELCAEVAQRIPVRRL
                     GQPVDIARAGAFLLSDDADYINGAILTMDGGTMVLPPW"
BASE COUNT         1268 a         2345 c         2433 g         1171 t
ORIGIN      
        1 ggggatgccg gttcgcctgg gcgcgcctct ggatcccgcg gcggcaacga gcgccctgag
       61 cggcccgtcg ttcgctacgg gggtgggcct ggtcacctac gcggcgcgct atgcccgtta
      121 cgcgcgccag cgtgagggac agttgttgga gggcctcttc gccgggatcg gccgctggct
      181 tcgcggcctc ctgtcgcgct aagagagagt gatgaagatg gattcgactt ccgccacgcc
      241 cgaggtagta aacatggcaa gctcgacagt tccgaaacag tgcgcggcca tcaaggtggt
      301 cgggatcggt ggcgggggat gcaatgccat caaccgcatg attgaggtgg gagtccaggg
      361 ggtggacttc atcgcgatga acacggacgc gatggccctg gagcactcgg cggccccgac
      421 gcggctgcag ctcggcccga aactgacgcg cggcctgggc acaggcggcg atccggaaaa
      481 gggccaactc gccgccgagg agagccgcca ggagattgtg cgcgttcttg aaggcgcgga
      541 tatggtcttc gtcactgccg ggatgggggg tggcacgggc accggcggcg cccccgttgt
      601 cgctgccatc gcgcgcgacc tgaaggcgct gaccgttggt gtcgtcacca agccgttcac
      661 caatgagggc ccccggcgag ggagggcggc acaggaaggg ctggcgcgat tgggcgagag
      721 ggtggatgcc ctcatcgcga ttccgaatga ccggcttcgg gagctttcga accgtcgcct
      781 ggcgctgcgc gatgccttcc tcatggctga tgatattctc cgccagggcg tgcagggaat
      841 tgccgagatc atctacgtca ccggcgactg gaacgtggac ttcgaggatg tgcgcaccat
      901 cgtgcagaat gccggcaccg ccttgatggg cattggcatc gcctcgggtc aggatcgagc
      961 cactaaagct gcgcaggccg ccatctccag tccgctgctg gagacctcca ttcagggtgc
     1021 ccgcggcgct ctcgtcaaca tcacggcgcc cgatgacttc ctgatcgagg agtttgacga
     1081 gatcacgcag ctgatccgca acgccaccga tcccgaagag gcaaacatca tcaccggtct
     1141 ggtgcaagga gcctcctccg agaccggcga ggtgaaggtg accgtgatcg ccaccggctt
     1201 tgggagtcca tcgccacggc ctcaggagac ggctgaggaa gctcgatcct ggggcgcggc
     1261 aatgccatca tccccgccgg catccagcgg cagtaacctg cgctcctggg gcttcgcgcc
     1321 cgtgtcgcgc ccggcatcgc ctgcgggtgg aaaccccgat tcgccatcga ccgccccgag
     1381 cgggcccgcc gatgcgaagc caccacaggc gaagtccgag ccggcccaga accctacgcc
     1441 ggtttctgac ttgctggatg aagtgcggcg cgcaacccgt gagatgggcg aagggcccta
     1501 tgatccgccg acgttcctgc gcgaccgcaa gcggcggtcc ggtggcaggg gatctggcga
     1561 gggcagataa atgggcggcc tggggcaagc cccggtcgcc aaagccccga cgagggagcc
     1621 cataacggcc cttcgcaggg attgagggag agaactgagc atgacgagtt accgggacat
     1681 cgagccgttt gcccggatca aagtggtggg ggtcggagga ggcggcagta acgccgtgaa
     1741 ccggatgatc cagatgggtg ttcagggcgt ggacttcatc gtcatgaaca ccgatgccca
     1801 ggcactcaag gtgtcgcagg ccccggtacg cctgcagatt ggcgcagagc tgaccggcgg
     1861 gctgggcacc ggggggaatc cggagaaggg gcagcgtgcc gctgaagaga gcaaacagga
     1921 gatcatgaag gcgctggatc aggccgacat ggtcttcatt accgccggca tgggcggcgg
     1981 cacgggcacc ggcgcatccc cggtcgtcgc gcaactctgc aaggagatgg gggcgcttac
     2041 cgtcggcgtc gtcaccaagc cctttgagaa cgaggggcct cgccgctggc gcttcgccga
     2101 agaggggctg gcccggctca aagagaaggt ggacaccctg atcaccatcc ccaacgaccg
     2161 cctgcgcgac gttgctgagc gccggctccc gctgcgtgag gccttccgca tggcggatga
     2221 cgttctgcgc cagggcgttc agggcatttc cgacatcatc caggtgcccg gcatgatcaa
     2281 cgtcgacttc gccgacgtgc aatcgatcat gagcaacgca ggcacggcgc tgatgggaat
     2341 tggcacggcc acgggcgaga accgcgcagt ccaggcagcc cagggcgcca tcgccagtcc
     2401 gctgctggag acctccattc agggagctaa gggggtgctg atcaacatca ccgcgtcgcc
     2461 cgacttcgcc atggaggagt atgacgagat caccagcctg atccgcggcg tgagtgaccc
     2521 cgacgatgcc aacatcatca ccggcgtggt gttcgacgag agcctgcagg atgaggtacg
     2581 cgtcaccgtc gtggccaccg gtttcggggc gacttccgcg agaagcgcct ttgaccaacc
     2641 cctcgcggcc acttccttcc cggtgacgcc ggtgcagccg ggccctgccg cgcaggcaac
     2701 gcccgcatcc gagccggcgc cgtcccaacg cgaggcaacc cgggtgctgg aggcgggcgc
     2761 gcgcgcgctc cgcgacctcg gcgacagtga tatcgacatt ccaaccttcc tgcgaccgcg
     2821 aagataggag cgttttgagc ccagacagca ctgtcgttcg gatcaaccgg ttgcgagata
     2881 ttgaaggaac acggaaggag gggcctcttt ccgtgttcct tcgcgctttt ctgggggctt
     2941 gcgccttctc cgcgcccctg cccggaggcg aaatcgaatg acctcctggg tggtgaagga
     3001 gcaagcgcgg gctcgattgg ctcgtgaaat cgttctcccc ggtggtggct tccgcgggcc
     3061 ggccggcatc cgcgcctgca tcctctaccc caacacctac cgcgtcggga tgtcgaacct
     3121 gggcttccag acgattcacg ccatcctcaa cgccacgcct ggtctctcgt gcgagcgcgc
     3181 cttcctgccc gaccctccag gacgcgccgg ccacggcaaa gcgcgcgatg ggatcatctc
     3241 cctcgaaacc caaactcccc tggccgagtt cgacctgatc ggcgtctcgg tgagctacga
     3301 gatggactac ccgaatgtga cgcgcgcgct tctggaggca gggatcccgc cgcttgcccg
     3361 cgatcgcgat gagcgccacc cgctggtcct ggccggcggg ccgtgcatca ccttcaaccc
     3421 ggagccgctg gccgagttcg tcgatctctg cgccatcggc gagggagagg agatcgtgcc
     3481 ggggattgcc gccgtgctgc gcgatgtgag tatggcgcgc ggggcttccc gcgccgagct
     3541 gctcgccgcg ctcgcggagc agccgggcgt ttacgtgccc tcgctctacg agccggaata
     3601 cactccagac ggcagcttcg ccgggccctc cccccaggca ggcgcgccgg agcgcatcga
     3661 gcggcagtgg gtgcgcgagc tggaccgtta cccctgcgcg tcgctgatcc tcaccccaga
     3721 gaccgagttc ggcgacctct tctgcatcga gatcagccgc gggtgcggcc gtggctgccg
     3781 ctactgcgcc gcgggctaca tcacccgccc accccgaatc cgctcccggg cacgtcttct
     3841 ggagggggtg cgcgccgccc ttccccaccg acgcaagatt ggcctggtca gcccggcgat
     3901 ctccgattac ccggacctgc ccgggctgtt gaacgacctg gcggagatgg gcgccgaggt
     3961 gtcgctatcg tcattgcgcg cggacacgct cctctcctct ccgacgctgg cgctcgcggg
     4021 ctcgggggaa tcggcgatca ccatcgcgcc ggaggcggga tcgcagcggc ttcgcttcgc
     4081 gctgaacaaa tcgctcacgg atgaggagat ctacgccgcc gtcgagaacg cgctcgccgc
     4141 ggggatccac aaggtgaagc tctacttgat gctcggcctg cccacggaga cggaggcgga
     4201 tatcgatgcc gccgttgaga tgtgccagcg gatcgccagc tatccgggga ttcgtcaggt
     4261 cacggtctcg gctaacccgt tcgttccaaa gccgggcacg ccgttccaat ggtgcgccat
     4321 gcagagcgag gcggtgctga gcgcgcgccg gaagcgcctg cacaccggcc tcaaaacgca
     4381 caagaagcta tcactcggcg gggaaagccc gcgagaagcg atcatccagg gcgcgctctc
     4441 tcggggagac cggcgcctcg ccccggttct tctcagtgtg gcgggggggg cagagctgga
     4501 acgccgcgtt ccgcgaggcc ggaatcgacc cggccgcgtt tgcctgccat gagatcgagc
     4561 cgggcgcgcc cttgccgtgg gatcacctcc acctgggcgc cggcgcggag tgcctgaaac
     4621 gagagtggtc catcgcccaa tcggccgcga cgcacccgcg gggtggactg acgcccgcct
     4681 gccagccggg cgcttgccgt cgttgcgagg cgtgttccaa cgcataagga ggattccctg
     4741 ctgccgcgcc taactctcca tcacgtaacc ggagggcaag cagatgacga cggtagcggt
     4801 cttcggcgcc ggcgggaagc tcggccgcca cgtgctgcgc gtcctggaga aacgcggcat
     4861 ggcggtgcgc gctctctgcc accggactcc catcgcggga gagaacgtga agagcatccc
     4921 cggaagcatc accgatgcag aagccgtgcg ggaggtggtc cggggtgcgg atctggtggt
     4981 gcagatggcc accacgaagg aggacccgga gacattcttc gacgtgagcg tgcgtggcac
     5041 gttcaatgtc ctggaagcgt gccggggcgc ccgtatccgc cagtttctcc ttttcggggg
     5101 cgacgcggcg atgggcatct ggttttaccc gcagccgatc cccatcgatg agaatcaccc
     5161 gctgatggcc tacccgggct actacgcctt ctccaaagtg atggaggagg tgatggccaa
     5221 tcagtatgcc attcaatacg gggtgccggt caccatcctc cgctcatcgt gggtttttga
     5281 gggcgcggac ctgctcaacc acttctcctt gctcaagaat atcaacccgg cggagccagg
     5341 ccatggcttc ggcgaggtga gccaggagat cctcgacctg gtgagtgcca gagaagagcg
     5401 gatcccgatc ctgacggacg gccagggagt gccccttcgc cggcacatcg tccatgtgga
     5461 cgacgtcatg caggcgttcg acaagatgct ggacgagccg gtggcaatcg ggcagagctt
     5521 taacatcgcc gggccggccc cgttcaacta ccgtgtcgcc gccaactacc tgtcggaaaa
     5581 gctcgggatc cccaccgtgg agatccgctg cccggcctac cacgcgttcg agatcaacat
     5641 cactcgagcg cgcacgatgc tcggctacgc cccggagaac gacttcttcc ggatggcgga
     5701 ccgggccata gaggaagcga tgtgaggtgc gggatacagg cggtcgcgtc gctcaagcgt
     5761 ggcggtgccg cgccaggtgc gtcgcggtcc gcccagacaa gccgctctac cacgcatcac
     5821 gtgtcatatc tccccagaga ggagcacccc aatgagaatg cagtttcagc acgtcggcct
     5881 gacgacagac gagaagcagc cgggagaggt attcgttccc gaaacgaagg tgtgggtcac
     5941 gagccctgat gatcaccctt accatatcga gtacctccgc tacgagccgg acagcccggt
     6001 gaagggcccc gtgcgcgatc tgccgcacat ggcgtttcgg gtgagcaacc tgaaggaagc
     6061 gatcggagac agcgagattc tcctcggccc cttcgaggcg gcgccggggc tgaatgtcgc
     6121 cttcgtcatg aaggatggcg cggtcttcga gtatatggag tacgaggacg agagcgcact
     6181 tccctggaag tgaggcggcc atgtcactgg aaggcaaggt gatcctcgtc accgggggca
     6241 cctcgggcat tggcgaggga tgcagccggc actacgccga ggtgggggcg aaggtagtca
     6301 tcgcctccaa ccaggagaaa gcaggtcgga aactggagga ggcgctgcgc gccgaggggc
     6361 gcgacgtgcg ctttgtctat accgacatca cctgtgagga gagcgtccgt aatctggtgg
     6421 cccaggccgt agcggcctat gggcgcattg atggggtcca ctgcaacgcc ggcgcctggg
     6481 cgccgggaaa ggtgaccgag ttcaacgatt ccgcctggga tctgatcatg ggagtgaacg
     6541 tgaagggggt tctctggacc gccaagcacg tcatccccgt catggagcga cagggcaaag
     6601 gcgtcctgct ggtcaccacc tccgtcgccg cgcacatcgg ctttccccaa catgtgatct
     6661 actgcgcgtc gaaggcggcg ctggaggcga tcatccgctg cctcaccacg gaccacgctg
     6721 gcgtggtccg cgtggtgggc atctcgcccg gcaccatcga cacgccgatg ctggccgcta
     6781 cctgcgcggg ctgggacaag cccaaggagg aactctgcgc ggaggtggcc cagcgcatcc
     6841 cggtacgccg cctcggccag cctgtggata tcgcgcgtgc cggcgccttc ctgctcagtg
     6901 acgatgccga ttacatcaac ggcgcgatcc tcaccatgga tggcggcacg atggtgctgc
     6961 cgccctggta gaatccgaaa tacacgcacc gggatggacc cgggcccgcc cgctctatct
     7021 ggacggcccg ggtccatcct atttccctat cacaacttga cgatctcgcc ggtcgccgcg
     7081 gaacgctcgg cggccagaca ggcgcgaaca gcttcgcgcg actcctccgg agtgatcacc
     7141 gtgggccgct ggccctgagc cacgcagttg gcgaagtagg ccagctcgtc gcgcagggct
     7201 ccggcccgcg ccccatg
//
LOCUS       DAOSCV010000077         7210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6646_length_7210_cov_3.020405, whole genome shotgun sequence.
ACCESSION   DAOSCV010000077 DAOSCV010000000
VERSION     DAOSCV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6646_length_7210_cov_3.020405"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLY56_03155"
     CDS             <1..639
                     /locus_tag="PLY56_03155"
                     /inference="COORDINATES: protein motif:HMM:NF020671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HOJ20507.1"
                     /translation="TLVEEGVLVALSAQAVVHRERWSALAERLVAVMDAFHQANPLRE
                     WMPREELHTRGARAVPGGTFGLMLQRLASEGRVEVQGNGVRRPGHAVSLTPEQEALAA
                     RIEARLGVTPFSPPSAAELVATEGPRAREILNALAEMGRVARVTPDLYLLADAIERIK
                     ALVSEHIAAHGQITAAAFRDLTGSSRKYAVPILEYLDSIRFTRRVGDERILW"
     gene            821..1393
                     /locus_tag="PLY56_03160"
     CDS             821..1393
                     /locus_tag="PLY56_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20508.1"
                     /translation="MLVIIAIIAILAGVLLPVLTRARSRGHQTACTHNLRQLYVAYTL
                     YERDWNGRFPPWHNLWTSAGAAVPEYRADLLHHALNTYVGNRDIWFCKSAVYKQKSVV
                     WCGIDQRYTGYVIPHVRWHHRLQRIPGSPYQTPDQVPLAGDPSGQPGDRDTPGLEYTG
                     APHFEGSVILWADGHVKWRPGSFQWQPLYP"
     gene            1651..2967
                     /locus_tag="PLY56_03165"
     CDS             1651..2967
                     /locus_tag="PLY56_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20509.1"
                     /translation="MYRIDPPAVYAHESTMAEPRLRERVERVCAALERPQQPVVYTDE
                     ELPELIQAGRWTDGLGAMGAKPEVRDPILVFNTFRFDDRLEERAKRFDEAKTGIGGHL
                     REMLLGDGAFIWFPAGLKEDPRRRQKVCRACWRIHFQAGCLHRCAYCGLGGILATMVN
                     VEEYLLHLGKLIERHPWQLTYLLEDDADVPCLEPELGCLGPIIEYFGTLQDRYLVIHT
                     KSWNVDWMLPLKHQGNTIIVWSLAGPTQSEVLEPKTGTTEERIEAARKCQEAGYTVRY
                     KFKPIIPVRGWREDAARTVEMALTRTNPDVISLCCFMWMDCDEMIRRLGRDRIDPEFL
                     AAAEASVEEMGTTVTRPFPEAMRAKIYDFYLKEIRKWNREVPVSLSTESWEMWERFRK
                     RLGASGSNYVCGCGPQSTPRLRKLPHGAWRDVVRNDEGLRGTGARI"
     gene            3017..4051
                     /locus_tag="PLY56_03170"
     CDS             3017..4051
                     /locus_tag="PLY56_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ20510.1"
                     /translation="MEKIRLGRTGLMVTRVGFGALPLQRVDRAEAARILCRAYDAGIN
                     FYDTARGYTDSEEKIGDALSGVRDRIVIATKSAAKSREGVLQDLEKSLRNLKTDYVDL
                     LQLHNPRSMPDPGDPESPYAGLLEAREKGMVRFIGFTNHSRERALQAVESGLFDTLQF
                     PFSAISSMEELALIGHCQQADVGVIAMKPLCGGLLTEARPAFAFLWQFENVVPIWGIQ
                     RMEELEESLALASDPPVLDAALRAEIERDRQELAGDFCRGCGYCQPCPEEIPIPMAAR
                     MSLLLRRSPYQPLLTEEWAERMMRIEKCRECGECMSRCPYGLEIPRLLQEMLADYKAF
                     RERHMPLPAE"
     gene            4397..5107
                     /locus_tag="PLY56_03175"
     CDS             4397..5107
                     /locus_tag="PLY56_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20511.1"
                     /translation="MAVLSRRSHGFTLIELLVVIAIIAILAAILFPVFAKARENARKS
                     SCQSNLKQIAMALLQYAQDYDETFINNLVYNPVTEKHDLRWHAVGVQPYVKNSGVLLC
                     PSTRREYGYSQYIGHGWSAANRVPASMATIAKPAQTVICADSFHQCMLPSSWRRPAGS
                     SCGAAYGNVTDACGWKTPDAPHMDGANFAFADGHVKWLKAEFSVYQPGSAAPPVTPTP
                     DFRKYEGGANDIWLTSEK"
     gene            5217..6044
                     /locus_tag="PLY56_03180"
     CDS             5217..6044
                     /locus_tag="PLY56_03180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20512.1"
                     /translation="MDESRSRLEREDLREEVRQIWDAQAEWWDDKIGDGNDFQRELVE
                     PATERLIGDVAGATVLDVACGGGRFGRRMAELGAHVVGIDISESFIRRARQRVPAHLA
                     ERLEYHVVDAADREALLLLGTARFDGAVATMALMDMAVIEPLFEALPAILKPGGWFVF
                     TLFHPCFEAPGVARFAESVEDETGIKTSTGVKMSRYLTPEAYRGIGILGQARTHYYFH
                     RPLQVLLGAGFRNGLVVDALEEPAYAPTDEPATLNSVWYRPTEIPRVLAVRMRVPRG"
     gene            6376..>7210
                     /locus_tag="PLY56_03185"
     CDS             6376..>7210
                     /locus_tag="PLY56_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20513.1"
                     /translation="MKRLLAPLLMLLFATTGAQPQLEDRDATVTVHPPKTRQIQKGRN
                     VTQDVTLATASASYTLRYDIVERSDDPQKIHFAKWAPTLGFTPLGLIGPSECLWYNQG
                     FFAWTFDGHNINDTRARFRVVRQHGQDAMVEYVWDTPKVTATIRFAVTAGSDKLLFVG
                     SYQPKTEIKEARLRLLSYPATFAKPWQRSVTTATRTLTTGKSVPLDLSKERWVLLEDT
                     LQGRMGDGSAGLILGDTTAFSRVTLDDIGGYAEYVNLTLAPGRRDFALALYAFPSIRD
                     PE"
BASE COUNT         1231 a         2277 c         2414 g         1288 t
ORIGIN      
        1 acgctcgttg aggagggggt gttggtcgcg ctttcagcgc aggcggtggt gcatcgcgag
       61 cggtggagcg cgctggcgga acgcctggtc gcggtgatgg atgcgtttca tcaggccaat
      121 cccctccgcg agtggatgcc ccgtgaggag ctgcacacgc ggggcgcgcg cgccgttccg
      181 gggggcactt tcgggctgat gctgcagcgg ctcgcgtccg aggggcgggt cgaggtgcag
      241 gggaatgggg tgcgccggcc ggggcatgcc gtctccctca ctccggagca ggaggcgctt
      301 gccgctcgca tcgaggcgcg cctgggggtg acgcctttct cgccgccgag cgcggcggag
      361 ctcgttgcca ccgagggccc gcgcgcgcgg gagattctca acgcgcttgc ggagatgggc
      421 cgggttgcgc gggtcacgcc cgatctctac ctgctcgccg atgccatcga gcggatcaag
      481 gcgctggtct cggagcacat tgctgcccat gggcagatca ccgccgcggc gtttcgcgac
      541 ctgactggct ccagccggaa gtacgccgtg ccgatcctgg agtatctgga ctcgatccgc
      601 ttcacccgtc gcgttggaga cgagcgcatc ctctggtagc caatcggctg cctcttgcat
      661 gatcgggaac agatgcaggc cggcgggcgt cgaacagata ggacgcgcgt cgttgaggct
      721 ttcagtcctg cagcgccaaa ctgagggcca ggagaggagg cgcgccgtga aacgaagcca
      781 gtggaggctc agtgcgccgc cgggtatcac gctcatggag atgctggtca tcatagccat
      841 catcgccatc ctggccggag tgctcctacc tgtgctcacc cgcgcgcgct cgcgcgggca
      901 tcagacagcc tgcacccaca acctgcgcca gctctacgtc gcttatacgc tctacgagcg
      961 cgactggaac ggccggtttc caccctggca caacctctgg acatcggccg gcgctgccgt
     1021 tccggagtac cgtgccgatc tcctgcacca cgcgctgaat acctacgtgg gcaaccgcga
     1081 catctggttt tgcaagagcg ccgtgtataa gcagaagtcc gtcgtctggt gcggcatcga
     1141 ccagcgttac actggctatg tgatcccgca cgtgcgctgg catcaccggc tgcagcggat
     1201 tccgggtagc ccctatcaga cgcccgacca ggtgccgctc gccggcgatc cgtccggaca
     1261 acctggcgac cgggatacgc ctggtctgga gtataccggc gcgccgcact tcgaggggtc
     1321 ggtcatcctc tgggcggatg gccacgtcaa atggcgcccc ggctccttcc agtggcagcc
     1381 cctttatccg tgaagtggcc taaacgacct ggcctgccag cgtggcgggc gctccctcct
     1441 cgcgcgttgg ggtaagactt accagcccca ggatggccgg caaccggtag gaactccatc
     1501 tccgtccggc gtatctgctg atcacgggcg cgccctgtcg gggcgccgtg tgcagcaggg
     1561 ggagggccag ggcgatgcag tccagaccca tcgcgagcct tcgccctcgt gtcgcgttcg
     1621 ctttcaggac gaatgaagga gtgagcgcct atgtaccgca tcgacccacc cgccgtgtat
     1681 gcccacgaaa gcacgatggc cgagccacgc ctccgcgagc gcgtcgagcg cgtgtgtgcc
     1741 gcgctggagc gccctcagca gccggtcgtc tataccgatg aggagctgcc cgagctgatc
     1801 caggccggac gctggaccga tggcctgggc gcgatgggcg ccaagcccga ggtgcgcgat
     1861 cccatcctcg tcttcaacac tttccgcttt gatgaccgcc tggaggagcg cgctaagcgc
     1921 tttgatgagg cgaagacagg catcggcggc catctccgcg agatgctcct gggcgacggc
     1981 gccttcatct ggttccccgc ggggctcaag gaggacccac gccggcgcca gaaggtgtgc
     2041 cgggcatgct ggcggattca tttccaggcg ggatgcctgc atcgctgcgc ctattgcggt
     2101 ctgggcggca ttctcgccac aatggtgaac gtcgaggagt acctgctcca cctcggcaag
     2161 ctgatcgagc gccacccctg gcagctcacc tacctgctgg aggacgatgc cgatgtgccg
     2221 tgcctggagc cggagctcgg ttgccttggt cctatcatcg agtatttcgg aacgctccag
     2281 gaccgctatc tggtgatcca caccaagagc tggaacgtgg actggatgct ccccctgaag
     2341 catcagggga acaccatcat cgtttggagc ctggcggggc cgacgcaaag cgaggtgctc
     2401 gagccgaaga cgggaaccac cgaggaacgc atcgaggcgg cgcgcaagtg ccaggaagcg
     2461 ggctacaccg tccgctacaa gttcaagccg atcatcccgg tgcgcggctg gcgtgaggat
     2521 gccgcacgga ccgtggagat ggcgctgacg cgcaccaatc cggatgtgat ctccctctgt
     2581 tgctttatgt ggatggattg tgatgagatg atccgccgcc tgggccgcga ccgcatcgat
     2641 ccagagttcc tcgctgccgc agaggccagc gtggaggaga tgggaacgac ggtgacacgc
     2701 cccttcccgg aggcgatgcg cgcgaagatc tacgacttct acctgaagga gattcgcaag
     2761 tggaaccgcg aggtgccggt gtcgctctcc accgaatcct gggagatgtg ggagcgcttt
     2821 cgcaagcgcc tgggtgccag cggctcgaat tacgtctgcg gctgcggccc gcaatcgacg
     2881 cccaggttgc ggaagctgcc tcatggagcc tggcgcgacg ttgtccgtaa cgacgagggt
     2941 ctgaggggca ctggcgcgcg catctgaggc cgtctgcggc gccggctgat gccggcataa
     3001 gactggggga ggatggatgg agaagatcag attgggccgc acgggcctga tggtcacgcg
     3061 tgtgggattt ggcgcgctgc cactgcagcg cgtggatcgc gcggaggcgg cgcgaatctt
     3121 gtgccgcgcc tacgatgccg ggatcaactt ctacgatacc gcccggggtt ataccgatag
     3181 cgaagagaag attggcgatg cgctgagcgg agtgcgtgac cgcatcgtga tcgccaccaa
     3241 gagcgctgcg aagagccgcg agggcgtcct ccaggatctg gagaagagcc tgcgcaacct
     3301 gaagaccgat tacgtggacc tgctgcagct tcacaaccct cgctccatgc cggacccagg
     3361 cgacccggaa tctccttacg cggggctgct cgaagcgcgc gagaagggga tggtccgttt
     3421 catcggcttt acgaatcata gccgcgagcg tgccctccag gccgtagaga gcgggctttt
     3481 tgatacgttg cagttcccgt tctccgccat ctcgtcgatg gaggagctgg cactcattgg
     3541 gcactgccag caggcggatg tgggggtgat cgccatgaaa cccctctgtg gcggcctgct
     3601 gacggaagcg cgccccgcct tcgctttcct ctggcagttc gagaacgtcg tgccgatctg
     3661 ggggatccag cgcatggagg agctggagga gtccttggcg ctggcctcgg atccgcccgt
     3721 gctggatgcc gcgctgcgcg ccgagatcga gcgcgaccgc caggagctcg cgggcgactt
     3781 ttgccgcggc tgcggctatt gccagccgtg cccggaagag atccctatcc cgatggctgc
     3841 gcgcatgagc ctcctcctgc ggcgctcgcc ctaccagccg ctcctcacgg aggaatgggc
     3901 cgagcggatg atgcggatcg agaagtgccg ggagtgcggc gagtgcatgt cacgctgccc
     3961 ttacggcctg gagatcccgc gcctgcttca agagatgctc gctgactaca aggcgttccg
     4021 agagcgccac atgcctctcc ccgccgaata gcgcgaggcg agcggcatca ctggagatat
     4081 cgcgcgggcc ttcgggtccg cgcctgtctc cttcatccgc ggtaccaccg ccattttcgt
     4141 tgacgggagc gatccgcgcg tgctataatc ggctatcaga gtaatgccgc ggagcactgc
     4201 cgttcgccgg ccagcctggc gcggggtgct ctcgtcgggc ctctcttccc gggccctatt
     4261 gcagatcagc gtggcctagg gccggcgcct tgccgcccag gggcgccatc ctggatctgt
     4321 ctgcctgcca ctcggcaggt ggctgccacc tgccagagca gctacccgcc tgttgcgtgg
     4381 acgaaaggag aggaccatgg ctgtactatc cagacggagc cacgggttta ccctgatcga
     4441 gctgttggtg gtgatcgcga ttatcgccat cctcgctgcc atcctcttcc ctgtctttgc
     4501 caaggcacgg gagaacgcac ggaagtcgag ctgccaatct aacctcaagc agatcgcgat
     4561 ggcgctgctt caatacgccc aggactacga cgagacgttt atcaacaacc tggtttacaa
     4621 cccggtcacg gaaaagcacg atctgcgctg gcatgccgtc ggcgtccagc cctacgtcaa
     4681 gaacagcggc gtcctcctgt gcccatccac ccggcgcgag tacggataca gccagtacat
     4741 cggccatggc tggtccgcgg cgaatcgagt gcccgcgtcg atggccacca tcgccaagcc
     4801 ggcgcagacg gttatctgcg ccgattcctt ccaccagtgc atgttgccca gctcgtggag
     4861 gcgtcccgct ggctcatcct gcggcgcggc gtacggaaac gtgacggacg cctgcgggtg
     4921 gaagacgccc gatgcgcctc acatggatgg ggcgaacttt gccttcgcgg acgggcatgt
     4981 caagtggctg aaagccgaat tcagcgttta ccaaccgggc tccgccgcgc ccccggtgac
     5041 gccgactccc gacttccgga agtatgaagg gggtgccaac gacatctggc tgacctcgga
     5101 gaaatgagag gcacgcgggg cgcacgcgtc gaaaagaatg cggtacgcct cgcgtgcctg
     5161 gcgagcaccc ctttgccgca ggccgggagg gagacaagcg gaagggagag cagaggttgg
     5221 acgaatcgcg gtcccgtctg gagcgggagg acctgcgcga ggaagtgcgc cagatttggg
     5281 atgcccaggc tgagtggtgg gacgacaaga ttggcgacgg gaatgacttc cagcgggagt
     5341 tggtggagcc agcaacggaa cgcctgatcg gcgacgtggc cggcgccact gtgctagacg
     5401 tggcgtgcgg gggagggcgg ttcggccggc gcatggcaga gctgggcgcg cacgtcgtcg
     5461 gcatcgatat tagcgagagt ttcatccgga gggccaggca gcgcgttccc gcccacctcg
     5521 cggagcgcct ggagtatcac gtggtcgatg cggccgaccg cgaggcgctg ctgctgcttg
     5581 gaacggcccg gtttgatggc gccgttgcca cgatggcgct catggatatg gctgtcatcg
     5641 agcccctctt tgaggcgttg ccggccatcc tcaagccggg tggctggttt gttttcaccc
     5701 tgtttcaccc ttgtttcgag gcccctggcg tggcccgctt cgccgagtca gtcgaggatg
     5761 aaacgggcat caagacgagc acgggcgtga agatgagccg ctatctcact ccggaggcgt
     5821 accgaggcat cggcatcctt ggccaggcgc gaacgcatta ctatttccat cgcccgttgc
     5881 aggtgctgct gggcgccggt tttcggaatg gtctggttgt ggatgccctc gaggagcccg
     5941 cgtatgcgcc aaccgacgag cccgccacgc tgaactcggt ctggtatcgc ccgacagaga
     6001 tcccccgggt gctggcggtg cggatgcgcg tgcccagagg gtgatcgtca ataccgcgga
     6061 tgaagggacg gacatggatg ggccggtgag ggcaccctcg gattacattc cccccggcgg
     6121 cctgttcgag acccgcgcgg tcgctcccct tctctggggc gggtgccaga ttacccgaac
     6181 gaacgcttat tagggcgagc tttttgtgtt cgccctgttt cgtcctatcc gttgccctgc
     6241 gtgacacgcg aagcccctgc aggaaacacc tcgctctgcg ggcaactttt ctaggtaact
     6301 attgtaggca gaacgagccg cgggaagccg cgctcgcatc catagcggcg actccggttc
     6361 gaggaggtac cagcgatgaa gcgattgctt gctccgctcc tgatgctctt atttgccacg
     6421 acgggtgcgc aaccccagct ggaggaccgt gatgccacgg tgacggtcca tccgccaaag
     6481 acgcgccaga tacaaaaggg ccgcaacgtc acccaggatg tcaccctggc gacggcttca
     6541 gcgagttaca cgctccgcta cgacatcgtg gagcgcagcg acgacccgca gaagatccac
     6601 ttcgccaagt gggcgccgac gctcggcttc acccccctgg ggctcatcgg gccgtcggag
     6661 tgcctctggt ataatcaggg gttctttgcc tggaccttcg acgggcacaa catcaacgac
     6721 acacgcgcgc gtttccgggt ggtgcgccag catggccaag acgccatggt ggagtacgtg
     6781 tgggataccc cgaaggtgac ggcgaccatc cgattcgcgg tgactgccgg cagcgacaag
     6841 ctcctcttcg tgggcagcta tcaaccgaag acggagatca aggaagcgcg cctgcgcctg
     6901 ctgtcctacc cggctacctt cgccaagcct tggcagcgga gcgtcacgac cgccacgcgg
     6961 acgctgacta cagggaagag cgtcccgctc gacctttcga aggagcggtg ggtacttctc
     7021 gaggacactc tccagggccg gatgggtgat ggatcggccg ggcttattct gggcgacacc
     7081 acggcgttct cgcgcgtgac tttggacgat atcggtgggt atgccgagta cgtgaatctg
     7141 acgctcgccc cgggccgacg cgacttcgcc ctggcgctct acgccttccc gagcattcgc
     7201 gacccggagg
//
LOCUS       DAOSCV010000078         7202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6657_length_7202_cov_3.812789, whole genome shotgun sequence.
ACCESSION   DAOSCV010000078 DAOSCV010000000
VERSION     DAOSCV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6657_length_7202_cov_3.812789"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="PLY56_03190"
     CDS             complement(<1..1059)
                     /locus_tag="PLY56_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ20514.1"
                     /translation="MGRTVIPELVRTPVTGVRLTDGLFRTAFENNIGFLKSLSQDAIL
                     YWFRVKAGLPAPGQPYRGHFEDNIKGQTAGLFLMGAGNSLRWQEDAELRARVNEIVDT
                     IDTAREPDGFFEPIPKAEFGTKEYPNYVRVWLNYGLTAAHLSGNPKALGLMRDMQSWF
                     NRCDERFLAKELLLGFQGVIANTTVYFSEVGLPEDLDVTVECYQENWWLAQLIRNDHG
                     AIYRRPTPHGTELEAITAYLDLYIATGKPLYLNAVNSAYRMFQEKWQHVGGGIVAIEY
                     TEITPGCLWLDPIHKYNELCCSAHWLYLNQRYHRLYPDGESYVGEIEKTLYNIIIANQ
                     AGTEHIRYHAYLDIQKDDD"
     gene            complement(1292..4495)
                     /gene="carB"
                     /locus_tag="PLY56_03195"
     CDS             complement(1292..4495)
                     /gene="carB"
                     /locus_tag="PLY56_03195"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ20515.1"
                     /translation="MPRREDIRKVLIIGSGPIIIGQACEFDYSGTQACKALREAGYEV
                     VLVNSNPATIMTDPGMADRTYLEPLNVERLEQIIAKERPDAVLPNLGGQTGLNLTAEL
                     HHRGILDRYGVQIIGVQADAIERGEDRIAFKDAMKRIGIEMPRSEPAYSVEEAERIAA
                     ELGYPVVIRPAYTMGGTGGGIAYNVEELRTVAARGIAASMVNQILVEESVIGWEELEL
                     EVVRDATGAKITVCFIENVDPMGVHTGDSYCVAPMLTVSKELQERLQDYAYRIVDAIG
                     VIGGTNVQFAHNPETGRVVVIEINPRTSRSSALASKATGFPIALISAKLASGISLKEL
                     PYWRDGTLDRYTPSGDYVVVKFARWAFEKFPGSLDQLGTQMRAVGEVMSIGKTYKEAF
                     QKAIRSLETRRYGLGFAKDFNTLPLEELRRRLVQPSSERQFLMYEALRKGMTVEELHG
                     LTHIGRWFIQQMKELVELEEEILKYKGTLPPDELLIQAKKDGFSDKYLGQILGIPEAQ
                     VRERRIALGVKQGWEAVPVSGADAAYYFSTYNAPDSVPVSPNKKVMILGGGPNRIGQG
                     IEFDYTCVHAAFTLRDLGYESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEKE
                     KPEGVIVQFGGQTPLNLAQELAANGVKILGTSPESIDLAEDRKRFAAVMRRLGIPQPE
                     SGTATSLEEALEVARTIGYPLMVRPSYVLGGRGMEIVYDEEMLRKYVTAAVEVTPDRP
                     MLIDKFLENAVEAEADAVADGTDAFVPSIMEHIELAGIHSGDSACVIPPTTIPEKHLS
                     TIVEYTKRIATELKVVGLMNIQYAIANDTVYILEANPRASRTVPLVSKVTGVSMARIA
                     TQLMMGATLRDLDLRTRKYPHFGVKESVFPFNMFPEVDPLLGPEMRSTGEVLGLADNF
                     GLAFYKSQVAAGTALPLEGTVLITVASRDKQAVLPAAQKLAEMGFTLLATDGTCRFLN
                     EHGVPARPVKKLYEGRPNLLDLLQNGEIHLVINTPAGKEAVTDDSYIRKAAVKGKIAY
                     ITTPAAAVAAAEGIAAARRGVGSVKSLQEYHDQIVVE"
     gene            complement(4901..>7202)
                     /gene="carB"
                     /locus_tag="PLY56_03200"
     CDS             complement(4901..>7202)
                     /gene="carB"
                     /locus_tag="PLY56_03200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOJ20516.1"
                     /translation="FDKFVTADHTITTQMKATGEVMAIDRTFEAALLKAVRSLETGTY
                     GLRSKNSHQLTEMQIKEGIRVPNHERLFIIAEAFRRGYTVEEIAQLSNIDRWFLRKIE
                     GLVKLEQEISVAASRNSWDYGTPGKAGQSGAAGGPASPCSPSRPLIPTDLLQRAKQMR
                     LPDRYLAEIAGVGEEEIRRLRQEAGIEPVYKMVDTCAAEFEAETPYFYSAMERESEFL
                     ESSRSPETDTENLEPGTLKPRKRAIVIGSGPIRIGQGIEFDYCSVHSAWALREAGYES
                     VIINNNPETVSTDFDTSDRLYFEPLTAEDVLEIIKREEPEGVIVQFGGQTAINLAEPL
                     HKAGVRILGSSFDSIDQAEDRNRFERLLNELNIPKPPGRAVTSVDAALQVAREIGFPV
                     LVRPSYVLGGRAMEIVNSEEELLSYMTYAVDVSPKAPILVDKYLLGKEVEVDLISDGV
                     DVLIPGIMEHIERAGVHSGDSMAVYPPQSIAPEVIDQVVSHAIALSRALEVRGLMNIQ
                     YVVENGVAYILEVNPRASRTIPYLSKITGVPMVRVATNVMIGKTLAEQGYSTGLWPAR
                     NLVAVKAPVFSFAKLHRVDIGLGPEMKSTGEILGIDTDFSRALYKAMIASGIAVPTEG
                     GTLLATIADRDKEEALPILQGFVDFGFRIVATAGTARFLKEHGINATAVRKIQEGSPN
                     MLDLVRSGEVALLINTLSNNKVSELDASKTRRASVELGVPCLTSLDTARALLMALRSQ
                     REGLQCLTIDEYVAASPPAGKPGVVQ"
BASE COUNT         1230 a         2279 c         2327 g         1366 t
ORIGIN      
        1 gtcatcatcc ttctggatat ccaggtaggc gtgataacgg atatgctccg tgccggcctg
       61 gttggcgatg atgatgttat agagtgtctt ctcgatctcc cccacgtagc tctccccgtc
      121 ggggtagagg cggtgataac gctggttcag atagagccag tgcgcgctgc agcagagctc
      181 gttgtacttg tggatgggat ccagccagag gcacccgggg gtgatctccg tgtactcgat
      241 ggcgacgatg ccgccgccca cgtgttgcca cttctcctgg aacatgcggt aggcactgtt
      301 gaccgcgttg agatagagcg gcttgcccgt ggcgatgtag agatccaggt aggcggtgat
      361 cgcctccagc tccgtaccgt ggggggtggg ccgccggtag atggcgccgt gatcgttgcg
      421 gatcagctgc gccagccacc agttctcctg gtagcactct acggtcacat ccaggtcctc
      481 gggaaggcct acctcggaga agtagacagt ggtgttggcg atgaccccct ggaagccgag
      541 gagcagctcc ttggcgagga agcgctcatc acagcggttg aaccacgact gcatgtcgcg
      601 catcagcccc agggccttgg ggttgcctga caggtgtgcc gcggtgagcc cgtagttgag
      661 ccagacacgc acgtagttgg ggtactcctt cgtgccaaac tcggccttgg ggatcggctc
      721 gaaaaagcca tcgggctccc gggcggtgtc gatggtatcg acgatctcgt tcacgcgcgc
      781 gcgcagctcg gcgtcttcct gccagcgcaa ggagttgccc gcgcccatga ggaagaggcc
      841 cgcggtctgg cccttgatgt tgtcctcgaa gtggcctcga tagggctggc caggcgccgg
      901 caggccggcc ttcacccgga accagtagag aatcgcatcc tgcgagaggc ttttcaagaa
      961 gccgatgttg ttctcgaatg ccgtccggaa gagcccatcg gtgagtcgga cgcccgtgac
     1021 gggagtgcgg actaactcgg gaatcacagt gcgacccata agccgtgcct ccgtctcgct
     1081 cgtgacgata cactacgagc attcgcctgg caaagagagg ggcgctatcc ggctacgcgc
     1141 ctgagcaccg ccggtatgcc gggggcatta tagcacaggg gtgtatgggc gtcaaacacc
     1201 acgatccaaa ggcggggggg cgcctgagca gcggagtcac ctcgcctgcc cgcgccccgg
     1261 gcccgtgcgg gcccggggcg ggacgcgagt gctattcgac cacgatctga tcgtggtatt
     1321 cctggagcga cttgacgctc ccgacaccgc ggcgcgcggc ggcgatgcct tcggcagcgg
     1381 ccacagcggc cgcaggagtg gtgatgtagg cgatcttgcc cttgacggct gctttgcgga
     1441 tgtagctgtc gtccgtcacg gcctccttgc ccgccggggt gttgatcacc aggtggatct
     1501 ccccgttctg gagcaggtcg agcagattcg gccggccttc atacagcttt ttcaccggcc
     1561 gggccggcac gccatgctcg ttcaggaagc gacaggtgcc atccgtcgcc aggagcgtga
     1621 agcccatctc cgccagcttc tgcgccgccg gcagcaccgc ctgcttatcg cgcgaggcca
     1681 ccgtgatcag caccgtgcct tcgaggggca gcgccgtgcc agcggccacc tgcgacttgt
     1741 agaacgccag gccgaagttg tcggccaggc caagcacctc gccggtggag cgcatctcgg
     1801 gcccgaggag cgggtcgacc tccgggaaca tgttgaaggg gaagaccgac tccttcaccc
     1861 cgaagtgcgg gtacttgcgc gtgcggagat ccagatcgcg caaggtcgcg cccatcatca
     1921 gctgggtggc gatccgcgcc attgagacgc cggtcacctt ggagacaagc ggcaccgtgc
     1981 gcgaggcgcg cgggttggcc tccaagatgt agaccgtgtc gttggcgatg gcatactgaa
     2041 tgttcatcag gccgaccacc ttgagctccg tcgcgatgcg cttggtgtat tcgacgatgg
     2101 tgctcaggtg cttctccggg atcgtcgtcg gcgggatgac gcacgcgcta tcgccggagt
     2161 gaatccccgc gagctcgatg tgctccatga tcgagggcac gaaggcatcg gtgccgtcag
     2221 cgacggcgtc ggcctctgcc tcgaccgcgt tctccaggaa cttgtcgatg agcatcggac
     2281 gatccggggt cacctcgaca gcggcggtga cgtatttgcg cagcatctcc tcatcgtaaa
     2341 cgatctccat gccgcgcccg ccgaggacgt aggagggccg gaccatcagc gggtagccga
     2401 tagttcgggc gacttccagc gcctcttcca gcgaggtggc ggtgccgctt tccggctggg
     2461 ggatccccag gcggcgcatc acggcggcga accgcttcct atcctccgcc aggtcgatgc
     2521 tctccggcga ggtgccgaga atcttcacgc cgttggccgc cagctcctgg gccaggttca
     2581 gcggcgtctg gccgccaaac tggacgatga cgccctccgg cttctccttc tcgtagatgc
     2641 tcagcacatc ttcgacggtg agaggctcga agtagagctt gttggacgtg tcgtagtccg
     2701 tagagaccgt ttccggattg cagttgacca tgatcgactc gtagcccagg tcacgcagcg
     2761 tgaaagcggc gtgcacacag gtgtagtcga actcgatccc ctgcccgatc cggttcggtc
     2821 ccccgcccag gatcatcacc ttcttgttcg gcgagacggg cacgctatcg ggagcgttgt
     2881 aggtggagaa gtagtaggcg gcatcagcgc cactcaccgg caccgcttcc cacccctgct
     2941 tcacgccgag cgcgatccgg cgctcgcgca cctgcgcctc gggtatgccc aagatctggc
     3001 cgaggtactt gtcggagaag ccatccttct tcgcctggat gaggagctcg tccggcggaa
     3061 gcgtgccttt gtactttagg atctcctcct ccagctccac cagctccttc atctgctgga
     3121 tgaaccagcg gccgatgtgg gtgaggccgt gcagctcctc aaccgtcatc cccttgcgca
     3181 gcgcctcata catcaggaac tggcgctccg aggagggctg gaccagacgc cgacgcagct
     3241 cctcaagggg cagcgtgttg aagtccttgg caaacccgag cccgtagcgg cgcgtctcca
     3301 gtgagcggat cgccttctgg aaggcctcct tataggtctt cccgatgctc atcacctcgc
     3361 cgacggcgcg catctgagtg ccaagttgat caagcgagcc ggggaacttc tcgaacgccc
     3421 accgcgcgaa cttcaccacc acgtagtccc cggagggggt gtagcggtcg agcgtaccat
     3481 cgcgccagta gggaagctcc ttcagcgaga tcccggacgc aagcttggcc gagatgagag
     3541 cgatcggaaa accggtcgcc ttcgaggcaa gcgccgacga gcgcgaggtg cgcgggttga
     3601 tctcaatgac gaccacccgt cccgtctcgg ggttgtgcgc gaactggacg ttggtgccgc
     3661 caatcacgcc gatggcatcc acaatccgat aggcgtaatc ctgcaggcgc tcctggagct
     3721 ccttcgagac ggtgagcatc ggagcgacac agtagctatc gccggtgtgc acgcccatgg
     3781 gatccacatt ctcgatgaag caaactgtga tcttggctcc cgtggcgtcg cggaccacct
     3841 ccagctcgag ctcttcccaa ccgatgaccg attcctcgac gaggatctgg ttcaccatgc
     3901 tggcggcaat gccccgcgct gcgacggtac gcaattcctc gacattgtag gcaatgcctc
     3961 cgccggttcc ccccatggtg taggccgggc gaatgaccac ggggtagcca agttcagccg
     4021 cgatgcgctc cgcctcctcc acgctgtagg ccggttcgct gcgaggcatc tcgatcccga
     4081 tccgcttcat cgcatccttg aatgcgatgc ggtcttcacc gcgctcgatc gcgtctgcct
     4141 gcacaccaat aatctgcacc ccgtaacgat caagaatacc gcggtggtgt agctccgcgg
     4201 tgaggttaag gcctgtctgg cctcctaagt ttggcaggac ggcatccggg cgttccttgg
     4261 caataatctg ttccaggcgc tcaacattca acggctcgag ataggtacgg tccgccatcc
     4321 ccggatcggt catgatcgtc gcggggttgg agttgacgag tacgacctcg tagccggctt
     4381 cacgcagcgc cttgcacgcc tgggtgccgg agtaatcgaa ttcgcatgcc tggccgataa
     4441 taatcgggcc ggagccgatg atgagtactt tgcggatatc ttcgcgtctg ggcatcgtgt
     4501 tgctgctccc ttggcatagg caacgagcgg cggcaagcca tccaccaggc tgagggagtg
     4561 ggcagcgccg ctgtctcgcg ttggaggtaa ggggatgcgt ccgggaggga gcgccggtca
     4621 aaccacaccg gtctgcagcg accctacgtc tgcactggcc cggcccgtcg accggccccg
     4681 tcctacctga tctctattcg tgaagagtct ctaatctcct tgttgttagg attccgaaag
     4741 gccctcgtcg ggaaagagac gcgtaagggc cagggtttgg gcaggggcga atgcttactg
     4801 ttcgccctgc tggatcttcg gcctccgcgc gaatgcctcg ccccgacaca agatccagcc
     4861 tgcgacaaat ggcaggggaa agctccatcg ccaggtgcga ctactgtacc acaccgggct
     4921 ttccggcggg tggcgacgcg gcgacgtact cgtcgatggt gaggcactgg aggccttcgc
     4981 gctgggagcg aagggccatc aggagcgcgc gcgccgtgtc gagcgaggtg agacagggga
     5041 cgcccagctc caccgaggca cggcgtgtct tgctggcatc gagctcggaa accttgttgt
     5101 tcgagagcgt gttgatcaac agcgcgactt ccccgctgcg caccaggtcc agcatgttgg
     5161 ggctaccttc ctggatcttc ctcactgccg tggcattgat gccatgctcc ttgaggaagc
     5221 gcgccgtgcc cgccgtggca acgatccgaa agccaaagtc cacgaagccc tggaggatcg
     5281 gcagggcctc ctccttgtcg cggtcagcaa tggtggccag cagcgtgcca ccctcggtgg
     5341 gcacagcgat gcccgaggcg atcatcgcct tgtagagcgc gcgggagaag tcggtgtcga
     5401 tgccgaggat ctcgccggta gacttcatct cggggccgag gccgatatcc acccggtgca
     5461 gcttggcgaa ggagaagacg ggcgccttca cggcgaccag gttgcgcgcg ggccacagcc
     5521 ccgtcgagta cccctgctcc gccagcgtct ttccgatcat cacgttggtg gccacccgca
     5581 ccatcggcac gccggtgatc ttcgaaaggt agggaatggt gcggctggcg cgcgggttca
     5641 cctccaggat gtaggcgacg ccattctcca ccacgtactg gatgttcatc agcccgcgca
     5701 cttcgagcgc ccgggagagg gcaatggcgt ggctcaccac ctggtcgatc acctccggcg
     5761 cgatgctctg cggcgggtag accgccatgc tatcgcctga gtggacgccg gcacgctcga
     5821 tgtgctccat gatccctggg atgagcacgt ccacgccgtc ggagatgagg tcgacctcca
     5881 cctccttgcc gaggagatac ttgtccacca ggatcggcgc cttgggcgag acgtcgacgg
     5941 cgtaggtcat gtaggagaga agctcctcct cgctattgac gatctccatg gcacgcccgc
     6001 cgaggacgta ggaggggcgc accagcacag ggaaaccgat ctcgcgcgcc acctgcagcg
     6061 cggcatccac ggaggtgacg gcgcggcccg ggggcttcgg gatgtttagc tcgttcagca
     6121 ggcgctcaaa gcggttgcgg tcctccgcct gatcgatgga gtcgaaggag gagccgagga
     6181 tgcgtacccc tgccttgtgc agcggctcgg ccaggttgat tgccgtctgc ccgccaaatt
     6241 ggacgatgac cccctctggc tcctcgcgct tgatgatctc cagcacgtcc tcggccgtga
     6301 gcggctcgaa gtagaggcgg tcggaggtat cgaagtcggt cgagaccgtc tccgggttgt
     6361 tattgatgat gaccgactcg tagcctgcct cgcggagcgc ccacgccgag tggaccgagc
     6421 agtagtcgaa ctcaatcccc tggccgatgc ggatcggccc actcccgatg acgatcgcgc
     6481 gcttgcgcgg cttcaaagtt ccaggttcca ggttctcggt atcggtttcc ggtgaccttg
     6541 aactctcgag gaactcggat tcccgctcca tggcgctgta gaagtacggt gtctctgcct
     6601 cgaactcggc ggcgcaggtg tcgaccatct tgtagaccgg ctcgatccca gcttcctggc
     6661 gcaggcgacg gatctcctcc tcgcccacgc cggcaatctc ggctaggtag cggtctggca
     6721 gacgcatctg cttggcgcgc tgcagcaggt cggtcgggat aagcgggcgg ctgggagagc
     6781 agggggaggc cgggccgcca gcggccccgc tctgcccggc cttgcccggg gtgccgtagt
     6841 cccaggagtt ccgggatgcc gccacggaga tctcctgctc cagcttcacc aggccctcga
     6901 tcttgcgcag gaaccagcgg tcgatgttcg agagctgggc aatctcctcg accgtgtagc
     6961 cgcgccggaa cgcctcggcg atgatgaaga ggcgctcgtg gttcggcacg cggatgccct
     7021 ccttgatctg catctcggtg agctggtgcg agttcttgct ccgcaggcca taggtgcccg
     7081 tctccagcga gcgcaccgcc ttcaacagcg cggcctcgaa ggtgcggtcg atggccatca
     7141 cctcccccgt cgccttcatc tgggtcgtga tggtatggtc ggcggtgacg aatttatcga
     7201 ac
//
LOCUS       DAOSCV010000079         7189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6679_length_7189_cov_2.992851, whole genome shotgun sequence.
ACCESSION   DAOSCV010000079 DAOSCV010000000
VERSION     DAOSCV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6679_length_7189_cov_2.992851"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="PLY56_03205"
     CDS             <1..563
                     /locus_tag="PLY56_03205"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOJ20517.1"
                     /translation="LAELKALDAGAWKDPKYAGERIPTLEEACRLAKGKVPLVLDLKG
                     DGYAEKIAEVVNRLEMSDQVIAACWTLQQAANIQRHLPLVVCKRIVSRGTPEQMADPA
                     FINTHLQAGLRGFSCYSQILSPLFIRAARRRAMPVFAWTVNDPGEMERMVMLGVDGIY
                     TDDPATLKQVLARLRQRTWAPAAPPQ"
     gene            966..3782
                     /gene="ileS"
                     /locus_tag="PLY56_03210"
     CDS             966..3782
                     /gene="ileS"
                     /locus_tag="PLY56_03210"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOJ20518.1"
                     /translation="MDVVDYRSTLNLPKTAFPMKGNLPTLEPQIQRRWEEMDLYRLSL
                     EKPAPNGRFILHDGPPYSNGDIHMGHALNKILKDLIVRYWSMQGYQAPFVPGWDNHGM
                     PIENNVSKEFRQKKVVPTRLELRRRCREYAQQWIETQREQFKRLGLRGDWSNPYLTMD
                     HAFEAKVVDIFGELAEQGYIYRGLKPVHWCPNDETALAEAEVEYGSHTSPSIYVRFPL
                     RRDPNGIFGDAPRERCYTIIWTTTPWTLPANLAVAVHPDEQYVVARMGDVYYLLAEAL
                     LKPTMEAIGITDMEVVSTHAGSDLAGLVFSHPFIDRDSVLLTATHVTMDAGTGVVHTA
                     PGHGREDFELGQANGLPALCPVDEQGRFTDEAGEFAGMDLKQGNTAVLEVLRERGALL
                     HESSLEHQYPHCWRCHQPTIFRATVQWFMRIDANDLRQRALMAIQGVEWIPEESINRI
                     NAMVANRPDWCLSRQRAWGVGIPVFYCTQCKHEILDRSAINAVRDLVLAEGSDAWFEK
                     DASEILPEGFTCPKCGATEFTKETDILDVWFDSGSTCRAVAEARPDLGYPSDLYLEGS
                     DQHRGWFNSSLMIGMGTRGMAPYRAVITHGMLLDAEGHAMHKSRGNAISPNQVIERYG
                     ADVLRLWVASTNYTEDVRLGDEILKRVTDVYRRLRNTLRYCLANLDGFDPVAHTVAYA
                     EMPEIDRWVLHRLQEVVSSVTDAYGSYAFYRAYHEIHNFCAVDLSAFYLDVIKDRLYT
                     SPADSLARRSAQTVLHELATALCTMLAPILVHTAEEAWGYLPAVEGRPESVHLAAFPV
                     VNEAFRDEELAARWSRLLDVREQVMKTLEEARRSGAISQPLEAHVRLRATGEWLELLR
                     RYEDELAAIFIVSRASVEEASSGEIEVAAEPATGLKCERCWLVREDVGTSHEHPTLCI
                     RCVDAVDLQQGGTDGSTAGSAPIP"
     gene            3748..4119
                     /locus_tag="PLY56_03215"
     CDS             3748..4119
                     /locus_tag="PLY56_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOJ20519.1"
                     /translation="MAQQLDLHRYREMLEKERQRMLRQIRTIDAGQDQELSESLSEIS
                     DYDDHPADVATETFEREKDRALRENARETLERVDAALRKMDTGTYGLCDICGQTIPAG
                     RLDAVPYATLCVKCQADVEGP"
     gene            4253..4810
                     /gene="lspA"
                     /locus_tag="PLY56_03220"
     CDS             4253..4810
                     /gene="lspA"
                     /locus_tag="PLY56_03220"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOJ20520.1"
                     /translation="MTRAPLAPDGSDGRGGRIGEGLQGNPAPVGWPRYVRYLCLSLLV
                     VAADQASKHAVLQGVGPDRYLPILGSTFGIRLVENRGVAFSLFASAGSFLIPLTLVAI
                     AAILYYARRAAGVHPWMLLALSLQLGGAVGNLIDRLRYGYVVDFLYLSFWPTFNVADI
                     AITLGAGLLAYCLLVSPDPARTESS"
     gene            4811..5881
                     /gene="lgt"
                     /locus_tag="PLY56_03225"
     CDS             4811..5881
                     /gene="lgt"
                     /locus_tag="PLY56_03225"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ20521.1"
                     /translation="MRPILFHIPVIPVAGWIALVAVFFGLSAYWGLRDIEAEGLSPEE
                     RGRRRSGMLAAHAVLFAIAAALLWHFQAAIAHPLPVRAYGFMLMTGFAAGLLYLKRAS
                     RGTALPPEGVVDLILGLLLVAIISSRFLFVLLQWESYSDDWREWFRVWEGGLSFHGGL
                     GGGILFVVWYARRYGLRFWWLADLIAPGLALGYAFARVGCFLNGCCYGRPTSLPWAVQ
                     FHDPPLSTHLTPPSHPVQLYAVIANLALFGILAHVWRRKRYDGQIAGLYLILYSVYRF
                     VAEGFRKGVTGNLFALGMTEAQWASLAIAAAGVALMVTMRKQATEGGPDPSGRSASRQ
                     PAALGKEQPAHGSTKRRAKKRK"
     gene            6008..7144
                     /locus_tag="PLY56_03230"
     CDS             6008..7144
                     /locus_tag="PLY56_03230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ20522.1"
                     /translation="MRVLHVLPPASGGMLRHLKCLVAGLTDVEPFCVAVPGAGLEALR
                     AMDRAPCAPLPLPNRATLSALLRASLVTATASRRWRPDLIHGHGYRGMLVAGVASCLA
                     RRPFLFTAHTLPNELGRGFWRLAGPLARRARAAACVSQAIQRGLAERLALPPAGSLSR
                     ALNSGMLRVIYNGIEPGPPPGPRVPLPADLGLPTARPVVITAARLAPQKGIAHLLDAW
                     ARLAPTFPEGILLLAGDGPLRPELEAQATVLGIGSSVRLLGFREDMDALLRAADLAVV
                     PSLAEGQSIFTLEAMARGVPVVATHVGGLPEMVREGRTGLLVPPADPPALATAMARLL
                     NDQELASAMAREARVLVEREWTVARMLAGYRDLYREVWHTVKQS"
BASE COUNT         1119 a         2395 c         2344 g         1331 t
ORIGIN      
        1 cgctggccga gctgaaggcg ctggatgccg gcgcctggaa agatccgaag tacgctggcg
       61 agcgcatccc tacgctggag gaggcctgcc gcctggccaa gggaaaggtg ccccttgtgc
      121 tcgatctcaa aggcgatgga tacgctgaaa agatcgccga ggtggtgaac cggctggaga
      181 tgagcgacca ggtgatcgcc gcctgctgga cgctacagca ggccgccaac atccagcgcc
      241 acctcccgct tgtggtgtgc aagcggatcg tctcgcgcgg cacccccgag cagatggcgg
      301 atcctgcttt catcaacacg catctgcaag cgggcctccg ggggtttagc tgctacagcc
      361 agatcctctc gccgctcttc atccgcgccg cccgccggcg cgcgatgccc gtcttcgcct
      421 ggaccgtgaa cgaccccggc gagatggagc ggatggtgat gctgggagtg gatggcatct
      481 atacggatga tccggccacg ctcaagcagg tactggccag gctccgccag cgcacctggg
      541 cccctgctgc cccgccgcaa taggcggcaa agcgccgccg catccgctca ggcggatgcg
      601 ggaaggcatc gcgcaggcag cgtgggcgta agcacccgcg ctgcctgcgc gcaaagccct
      661 gcgggcctac gttcccgagc aatccagggt accgggggct cggatcggaa agcagaggcc
      721 cgaaaggctt tcgctcggta gcgacgcgtg tgttaaggcc cgcgctactt tagtcccgca
      781 ccgatcaggc gctttacaga aggcatggtt tgtgatacca tatatcctgt agtacacgtt
      841 tcactggcag cgttcttttg tggcgagggc tctgcggagg gcgtcgcggt cggtccgcct
      901 cccggatagc gcctcgccat tgtgcatcgg tgggtccccg cgcccggcgg ggctccaagt
      961 agaggatgga tgtcgtggat taccgcagca cattgaacct acccaagaca gcatttccca
     1021 tgaaggggaa tctccccacg cttgagccac agatccagcg gcggtgggag gagatggatc
     1081 tctaccgact ctcgctggaa aagccggcgc caaacgggcg cttcatcctg cacgacggtc
     1141 ctccctactc taacggcgat atccacatgg gccatgccct caataagatc ctgaaggatc
     1201 tgatcgtccg gtattggtcg atgcagggct accaggctcc cttcgtgccg gggtgggata
     1261 accacggcat gccgattgag aacaacgtct cgaaggagtt ccgccagaag aaggtagtgc
     1321 ccacccgtct ggaactgcgc cgccgctgcc gcgaatacgc gcagcaatgg atcgaaactc
     1381 agcgcgagca gttcaagcgt cttggtctcc gcggcgattg gtcgaatccc tacctcacca
     1441 tggaccacgc cttcgaggca aaagtggtcg acattttcgg cgagctcgcg gagcagggct
     1501 acatctaccg cggcctgaag ccggtgcact ggtgcccgaa cgacgagact gcgctggcgg
     1561 aggcggaggt ggagtatggg tcgcacacct cgccctcgat ctacgttcgc ttcccgctgc
     1621 ggcgtgatcc caacggcatc ttcggcgatg ccccccgtga gcggtgctac acgatcatct
     1681 ggaccaccac gccttggacg ctcccggcga accttgccgt ggcggttcat cccgatgagc
     1741 agtacgtcgt ggcgcgcatg ggcgacgtct attacctgct cgccgaggcg ctcctgaagc
     1801 cgacgatgga ggccattggc atcaccgata tggaggtggt ctccacccac gcgggctccg
     1861 acctggcagg gctcgtcttc agccacccct ttattgaccg ggattccgtg ctcctgaccg
     1921 ccacccacgt caccatggat gccggcaccg gcgtggtgca tacggccccc ggacatggcc
     1981 gcgaggactt tgaactcggc caggccaacg ggctgccggc gctctgcccg gtcgatgagc
     2041 aggggcgctt caccgacgag gccggcgagt tcgccggaat ggatctgaag caggggaaca
     2101 ccgcggtgct cgaggtgctg cgcgagcgcg gggccctgct ccatgaaagc tcgctggagc
     2161 accagtatcc ccactgctgg cgctgccacc agcccaccat cttccgtgcc accgtgcagt
     2221 ggttcatgcg gatcgatgcc aatgacctgc gccagcgcgc gctgatggcg attcagggcg
     2281 tcgagtggat tcccgaggag agcatcaacc gcatcaacgc catggtcgcc aaccggcccg
     2341 attggtgtct ctcgcgccag cgcgcctggg gcgtcgggat cccggtcttt tactgcaccc
     2401 agtgcaagca tgagattctg gaccggagcg ccatcaacgc cgtgcgcgac ctggtcctgg
     2461 ccgaaggctc ggatgcgtgg tttgagaagg acgccagcga gatcctgccg gagggcttca
     2521 cctgccccaa gtgcggcgcc accgagttta ccaaggagac cgatatcctc gatgtgtggt
     2581 tcgattccgg ctccacctgc cgtgccgttg ccgaagccag gccggatctc ggctatccga
     2641 gcgacctcta cctcgagggc tcggaccagc atcgcggctg gttcaactca tcgctgatga
     2701 tcggcatggg cacgcgcggg atggctccct accgcgcggt catcacccac ggcatgctcc
     2761 tcgatgccga gggacacgct atgcacaagt cgcgcggcaa cgcgatctcg ccgaaccagg
     2821 tgatagagcg ctacggcgcc gatgtgctcc gcctctgggt cgcttccacg aactacaccg
     2881 aggatgtgcg cctgggcgat gagatcctga agcgcgtcac cgatgtctac cgccgcctgc
     2941 gcaacaccct gcgctactgc ctggccaacc tggatggctt cgatcccgtg gcgcacaccg
     3001 tcgcctacgc ggagatgcct gagatcgacc gctgggtgct gcatcggctg caggaagtgg
     3061 tgagctcggt gaccgacgcg tatggctcct acgccttcta tcgcgcctat catgagatcc
     3121 acaacttctg cgccgtggac ctgagcgcgt tctacctgga tgtgatcaaa gaccgtctct
     3181 acaccagccc ggccgactcg ctggcgcggc gctccgcgca gaccgtgctg cacgaactgg
     3241 cgaccgcgct gtgtaccatg ctggcgccaa tcttggtgca caccgccgag gaggcatggg
     3301 gctacttgcc ggccgtcgag ggtcggcctg agagcgtgca tcttgccgcc tttcccgtcg
     3361 tcaacgaggc gttccgggac gaggagctgg cagcacgctg gtctcgcctg ctggatgtgc
     3421 gtgagcaggt gatgaaaacc ctggaggagg cacggcgaag cggggcgatt tcccagccgc
     3481 tcgaggcgca tgtccgcctt cgggccactg gcgagtggct cgagctgctg cgccgctatg
     3541 aggatgaact cgccgcgatc ttcatcgtct cgcgcgccag cgtggaggag gcctcctcgg
     3601 gtgagatcga ggtcgcggct gaacctgcga ccggactgaa gtgcgaacgc tgctggctgg
     3661 tccgtgagga tgtgggcacc agccacgaac accccactct atgtatccga tgcgtcgatg
     3721 cagtagacct tcagcaagga ggcaccgatg gctcaacagc tggatctgca ccgataccgt
     3781 gagatgctgg agaaggagcg gcagcggatg ctgcgccaga ttcgcaccat tgatgctggc
     3841 caggatcagg aattgtcgga gtcgttgagc gagatctccg attacgacga ccaccctgcc
     3901 gatgtcgcga cagagacctt tgaacgcgag aaagaccggg ccctgcgcga gaatgcacgc
     3961 gagaccctgg agcgcgtgga cgcggcgttg cgcaagatgg ataccggaac ctatgggctg
     4021 tgcgatatct gcggccagac aattccggcg ggccggctcg atgcggttcc ctacgcgacg
     4081 ctctgcgtga aatgccaggc ggatgtggag ggcccctagt gggtcgttcg ccacgcgcga
     4141 cttcattggc tggcgagggc ctctctctgt ccgcgtccat ctcctgttcg ggctgtgggc
     4201 gcggacagag gcgggccccg atcgatgccg tgccactcga aaggccactg gcgtgacgcg
     4261 cgctcctttg gcgccagacg gcagcgacgg ccgcggcggt cggatcggcg aggggttgca
     4321 gggcaatccg gcaccggtgg ggtggcctcg ctatgtgcgc tacctgtgcc tgtcgctact
     4381 cgtcgtggcc gccgatcaag cgagcaagca cgccgtgttg cagggggtgg gcccagaccg
     4441 ctatcttccg atcctcggca gcaccttcgg gatccgtctg gtcgagaacc ggggcgtggc
     4501 gttcagcctc ttcgcgtcgg ccgggtcgtt cttgatcccg ctgactctgg tggccatcgc
     4561 cgccattctc tattatgccc gccgggccgc gggcgttcat ccctggatgt tgctcgccct
     4621 ctcgctgcaa ctcgggggcg ctgtcggcaa tctgatcgat cgccttcgct acggctacgt
     4681 cgtcgatttt ctctatctga gtttctggcc cacgttcaat gtggccgata ttgctattac
     4741 tctcggggcc ggactccttg cctactgctt gctggtatcg cccgaccccg ccaggacgga
     4801 gtcctcatag atgcgaccga tcctgttcca tattcccgtg atcccagttg ccggctggat
     4861 cgcgcttgtc gctgtgttct ttggcttgag cgcctattgg ggcctacgtg acatcgaggc
     4921 cgagggcctc tccccggagg agcgcggccg ccggcgctcg ggcatgctcg cggcccacgc
     4981 cgtcctcttt gctatcgccg cggcgctcct gtggcacttc caggccgcca ttgcccatcc
     5041 cctgccggtt cgagcctacg gcttcatgct gatgaccggt ttcgccgccg ggctgctcta
     5101 cctgaagcgc gcgagccgcg gcacggcgct cccacccgag ggggtcgtcg acctcatcct
     5161 cgggttgctc ctggtcgcta tcatctcatc gcgtttcctc ttcgtgctgc tccagtggga
     5221 gagctattcc gatgactggc gcgagtggtt ccgcgtctgg gagggcggtc tttccttcca
     5281 cggcggcctg gggggcggca tcctcttcgt cgtctggtat gcccgtcgct acgggttgcg
     5341 tttctggtgg ctcgccgacc tcatcgcccc ggggcttgcg cttggctacg cctttgcgcg
     5401 cgtcggctgc tttctcaacg gctgctgcta tggccggcca acctcgctcc cttgggccgt
     5461 ccagtttcac gacccgcccc tttcgacgca tttgacaccg ccgagccatc cagtgcagct
     5521 ctacgcggtg attgccaacc tggcgctctt cgggatcctc gcccacgtct ggcggcgaaa
     5581 gcggtatgat ggccagatcg ccgggctcta cctcatcctc tactccgtct accgcttcgt
     5641 ggcggagggg ttccgcaagg gcgtcaccgg caacctcttc gcgctgggga tgaccgaagc
     5701 ccagtgggcc agtctcgcca ttgccgccgc cggcgtggcg ctgatggtca ccatgcgcaa
     5761 acaggcgacc gaaggcgggc cggacccctc ggggcgatcc gcgtcgcgcc agcctgccgc
     5821 gctgggtaag gagcaaccgg cccacggcag taccaagcga cgggccaaga agcggaagta
     5881 gaggggccgt ggcttgcaga tggtcggaag ctgggcgagg aggtcgccac ctggcttcgc
     5941 ctcttcggcg gcactcagcc agggtcgctt cgcccggacc cgcggcgggg cggtgaggta
     6001 ggagaggatg cgcgttcttc acgtgctgcc gccggcctcg gggggcatgt tgcggcatct
     6061 gaaatgcctg gtcgcgggcc tgactgatgt ggagccgttc tgcgtcgcgg ttcccggcgc
     6121 cggcctggag gcgctccgcg ccatggatcg ggccccctgc gctcctctcc ctcttccgaa
     6181 tcgcgccacg ctgtcggcgc tccttcgcgc cagcctggtg accgcgacgg cgtcgcggag
     6241 gtggcgtccg gatctgatcc acggccacgg ctatcgcggc atgttggtag ctggagtggc
     6301 ctcatgccta gcccgcaggc cgttcctctt cactgcgcac acgcttccca acgaactcgg
     6361 gcgtggcttc tggcgtcttg ccgggccgct cgcgcgccgg gcccgcgccg ccgcatgcgt
     6421 ctcgcaggcg attcagcgcg gtcttgccga gagactggcg cttcctccgg ccggctcgct
     6481 ctcgcgcgcg ctcaactccg gaatgctccg cgtcatctat aacgggatcg agcccggccc
     6541 gcctccgggg ccgcgcgtgc cgctgcccgc ggatctcggg ctccctaccg ctcgacccgt
     6601 ggtaatcacc gccgcgcgtc tcgccccgca gaaggggatt gcccacctgc tcgatgcctg
     6661 ggcgcgcctc gcccccacgt tccccgaggg cattctcttg ctggccggag acggcccgct
     6721 gcgcccggag ctggaggcgc aggcgacggt gcttggcatc ggctcatcgg tgcgcctcct
     6781 cggcttccgg gaggacatgg atgccctgct gcgcgcggct gacctggccg tcgtcccttc
     6841 gctcgctgaa ggacaatcga tcttcactct ggaagcaatg gccaggggag tgccggtcgt
     6901 cgccacccat gtcggaggcc ttccggagat ggtgcgcgag ggccgcaccg gcctcctcgt
     6961 cccccctgcc gacccaccgg cgctcgcaac ggccatggcc cgcctgctca acgaccagga
     7021 actagcctca gcgatggcga gggaggcgcg cgtgctcgtt gagagggagt ggaccgtcgc
     7081 gcggatgctg gccggctacc gcgatctcta cagggaagta tggcatacgg taaagcaatc
     7141 atgacgggag gtgaccggat tccggcacct gctctcggtg ttcggccgg
//
LOCUS       DAOSCV010000080         7176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6695_length_7176_cov_2.899031, whole genome shotgun sequence.
ACCESSION   DAOSCV010000080 DAOSCV010000000
VERSION     DAOSCV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6695_length_7176_cov_2.899031"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLY56_03235"
     CDS             complement(<1..393)
                     /locus_tag="PLY56_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ20523.1"
                     /translation="MKPYEPGQIRNVALISHGGAGKTSLAEAMLFDAGVTTRMGRVEE
                     GNTVTDYEEDETKRQISVSAALAPLEWKGHKLNIIDTPGFIDFQADVRSALSVVEGAV
                     VLVDAVAGVEVGTELYWRLAEQNNLARIF"
     gene            1155..2585
                     /locus_tag="PLY56_03240"
     CDS             1155..2585
                     /locus_tag="PLY56_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ20524.1"
                     /translation="MEWILHGTVGILGAVLAVWIFGHAAYSWGLVRVRVSVAPARHGD
                     TAIVLTPIGEITARTHRAPVRLTVQLDRLHLGAVRRMAKEIPDASEVLAELEQRACRS
                     IGRYAFRLLLLALAGGTLASLMLLRGSGPWHLFSGFVGLAATSLVLVATVGTYDRGGF
                     ASPRYTGPLAEAPGAIRFARDGLARLSRLRGRMDTIAASLARFYASLGDSGPRVPAQT
                     DLRVLHISDLHNNPVAIDFVERLARHFQVDLIVNTGDITDYGTEVEEAFIQRLRQLPV
                     PQLLVAGNHDSQTTLRTLDREAEVRVLDGESVVVHGIRFAGWPDLVSKRQGYGSVNPS
                     SEDLDRLQKEIREGVAALPDRPHVLLVHNHRVAEQLGGVAPVILYGHNHRPSVRRTPR
                     STFINAGTTGGAGARYFEARNGVPYSAVVLHFSREEPGHLLAADLLQVHGPEGEFVLQ
                     RHMLNGTMNREKHLLPAPPPAHAGQP"
     gene            2719..>4052
                     /locus_tag="PLY56_03245"
     CDS             2719..>4052
                     /locus_tag="PLY56_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20525.1"
                     /translation="MRVLLLFLLAITTTAAGAAEKDAGLLPNPGFDKEMSGWVFRPGD
                     PSLAAIVDAGGERGKVLELHPDGKILGVETEKLELGKALEADAAYEVTALLKNEEIKN
                     GVFAFSMYCYDAAGKALRQIAFYGLNTKSAPHDWKRVRGEFGKGSANPLPEGTASICI
                     RFSFYEKSGNCQAKVLVDDVILKKHQPAVKNDWPAEVVADVGDLQVRFESRSFWTLYR
                     IDYQGTRLCHDRWGSHYGTVAFFPGVGFIGSGHTENENEEVLSRALEVDGKPVAQPET
                     TYRCRKIVLRKESKVRTLHYKTTITVADNRIWEEVILKADEPTKVDLIYHFMHPWTHT
                     ATEYLAEMPDGTRVEGSFTDSKKLLLDKPAKWSAIYDAPSGKGAVTYVLEVPEGQPWS
                     TLYWDQPERYRKHYLRTFVKSTVPANEELRYRIVTVPFAAAAEKWKEEAAKVA"
     assembly_gap    4053..4152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4153..4831
                     /locus_tag="PLY56_03250"
     CDS             <4153..4831
                     /locus_tag="PLY56_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20526.1"
                     /translation="LLDLVDANTVDLPVASLLAGAVASGASLLTAARPGNAGKTTVLA
                     ALLSFIPPGRRIVTVPDGPPRSVPPDTCLLAHEIGSGPWYAYLWGGAARGFFEAAAAP
                     GVQAASCIHADTLEELRMTLCGPSIGLGEKEFAAIDLVAFLRLDRTLTGYRRRVCAVY
                     AATGTPGAMSHQQICRWNEATDSFTVDAVPERWAAVSERAAEFLEAARTAGCTRFEEL
                     FARWATT"
     gene            complement(4835..5251)
                     /locus_tag="PLY56_03255"
     CDS             complement(4835..5251)
                     /locus_tag="PLY56_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20527.1"
                     /translation="MAVLIGALLAGCGGGGGDEQKADYDPPRIVWARFEPSSLGDIGG
                     TARIIALVTDASGVAWVRATLTRPDGSQAVHDMTWNQAGYYEVSIQVPANLSSADQRY
                     YASISAADIRGIASQAVSISPLTVRSDSAPPPPPKL"
     gene            complement(5521..6672)
                     /locus_tag="PLY56_03260"
     CDS             complement(5521..6672)
                     /locus_tag="PLY56_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20528.1"
                     /translation="MTSRERILTTLRHQEPDRVPFDLGSTKVTGIHRIAYERLRPALG
                     LAPREAAISDLTQQLAAVDEDLLQALRVDTRGVASGDPSGWRLCIREEGRYRTFTDAW
                     GLPRRMPRDGWYYDLCGHPLAEATSPEEIRRYPWPDPQDPARFAGLRARAQVARDTGC
                     AVILGGVCAGLTEMCLWLRGFERFFTDLVADRPMAEAMLDLILETKLRYWERALAEVG
                     DLVDIAMEGDDLGMQQCLLMNPRVYREVVKPRQAQLFAHIKKQAPVFVFYHTCGSVVE
                     ILPDLIEVGVDILNPVQVSAAGMDTRRLKAEFGRDIVFWGGGVDTQRILPHGTPQDVR
                     DEVRRRIDDLAPGGGFVFNTVHNIQADVPPENILAMWETLQEYGVGKAE"
     gene            complement(6669..>7176)
                     /locus_tag="PLY56_03265"
     CDS             complement(6669..>7176)
                     /locus_tag="PLY56_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ20529.1"
                     /translation="PIPVRVAISGCAEACSCPQTADIGFVGIVEPILVRGECQGNGTC
                     VEACKEGALIMRRGLPRRDPQRCNFCGDCVAACGFNALRPARVGYTVYVGGKAGRRPC
                     LGAELARFVPEEDAPEVAARVIGFVSERARPRETFGAVLRRIGLDALRSYAITRPSQR
                     TRPQEMTP"
BASE COUNT         1225 a         2407 c         2243 g         1201 t
ORIGIN      
        1 gaagatgcgc gccaggttgt tctgttctgc caggcgccag tagagctcgg tgcctacctc
       61 tactccggcc acggcgtcca cgagcacgac cgccccttcg acaacgctca gggcgctgcg
      121 aacatccgcc tggaaatcga tgaagcccgg agtatcgatg atgttgagct tgtggccctt
      181 ccactccagg ggagcgagtg ccgcgcttac ggagatctgt cgtttggtct cgtcctcttc
      241 gtagtcggtg accgtgttgc cctcttcgac tcgtcccatg cgagtggtga cgccggcgtc
      301 gaagagcatg gcctctgcaa gcgaggtctt gcccgctccc ccatgggaga tcagggcgac
      361 attcctgatt tggccaggtt catacggttt catcaatatc catctccttc agcaagtgcc
      421 ctccgggtat ggagatcacg gaagcaggga cgcccgggcc acccgctcgt ttccatacac
      481 tgaccccact tcaccgcagg cagccgcacg gcggcctcct gggcgaagag caagtcaacc
      541 aaccatttcc tcgcaaggat cggctctgca ggcaacgcag cgccttctgg ggcaggcttc
      601 ggctcacatc ctggttgagc ctggcccgga ggcccgctct cgcggatgtg caagcttccg
      661 ggtcgatagg gaaaaccctc cgtcccatcc acgcgtgccg ttcaacgtgt attctctttt
      721 cgatgcgcac cagccttctc cccccatcgt ctggcggaag ttgctgcgcc cgagggttct
      781 ggctgcgttc tccccggtaa agcaaagggt ccaggccacg agaagatccc gcgctgacct
      841 ggacccttca aaaatcagag cgcccaaggg aacgtccccc tccaggtagc cccggcgaca
      901 ccccgttcgc caccgggtag cccgggcaaa gtgagagcgc actatcacgc gcagtcgggc
      961 ttttctgcat ccaaagagca ggggtatccc cccttttgtt cattatatgg gggtagccgc
     1021 ttccttgtca agatcggcag ctcaacgcgc gatgatgcgg aaccgagtgc ccctcgttct
     1081 gtcctcagtc gtgaggattc cgcgggacgt ggtgtgcgga aggtggtctg gtgttcagga
     1141 agcagaggcg agggatggag tggatactgc acgggaccgt cggcatcctt ggcgccgtgc
     1201 tggctgtctg gatcttcggg cacgccgcct attcgtgggg gcttgttcgg gtgcgtgttt
     1261 ccgtggcccc agcgcgccat ggcgacaccg cgattgtgct cacccccatc ggcgagatca
     1321 ccgcgcgcac gcatcgcgcc cccgtgcgcc tcaccgtgca gctcgaccgg ctccatctgg
     1381 gcgctgtgcg tcgcatggcc aaggagatcc ccgacgccag cgaagtgctc gccgagctgg
     1441 aacagcgcgc ctgccgcagc attggtcggt atgcattccg cctgctgctc ctggcccttg
     1501 ccgggggcac gctcgccagc ctcatgctgc tgcgcggatc cggtccctgg cacctcttct
     1561 ccggctttgt cggcctcgcc gccacctcac tggtgctcgt cgccacagtg ggcacctacg
     1621 atcgcggcgg cttcgcgagc ccgcgctata ccgggccgct ggccgaggcg cccggggcaa
     1681 tccgcttcgc gcgcgatggc ctcgcccgcc tcagccggct tcgcgggcgc atggacacca
     1741 tcgccgctag cctggcgcgt ttctacgcct ccctgggcga ctccggcccc cgggtgcccg
     1801 cccagaccga cctacgcgtg ctccatatca gcgatctcca caacaacccg gtcgcgatcg
     1861 atttcgtgga gcgcctagcg cgccactttc aggtagatct gatcgtgaac accggcgata
     1921 tcaccgacta tggaaccgaa gtggaagagg cgttcatcca gcgcctgcgc cagctaccgg
     1981 tacctcagct cctggtcgcc ggcaaccacg attcgcagac caccctccgt acgctggatc
     2041 gcgaggccga ggtgcgcgtg ctggatggcg agagcgtggt ggtgcacggg atccgcttcg
     2101 cgggctggcc ggatctggtg tcgaagcgcc agggctatgg cagcgtgaac ccctccagcg
     2161 aagacctgga tcgccttcag aaagagatac gcgagggcgt cgccgccctg cccgaccgcc
     2221 cacatgtgct cctggtccat aaccaccgcg ttgccgagca actcggtggc gtagctccgg
     2281 tgatccttta cgggcacaac caccgcccgt cggtacgccg cacgccccga agcaccttca
     2341 tcaacgccgg cacgaccgga ggcgccggcg cccgctactt cgaggcgcgc aacggcgtgc
     2401 cctactccgc cgtcgtcctc cacttctccc gcgaggagcc ggggcacctc ctggcggcag
     2461 atctgcttca agtgcacggc cccgaaggcg agttcgtcct ccaacgccac atgctcaacg
     2521 gtaccatgaa tcgggagaag catctcctcc cggcgcctcc gccggcgcac gccggccagc
     2581 catagcggcg tttcccttac gaacccctcg cgctcgcctc ttccacagac ggcatcccac
     2641 gaggagttcg ccaggccagc ctggaataga ctcgccaaga gagcgccatt cggcgccgtt
     2701 ttcacagaga gagacaccat gcgagtgctg cttctattcc ttttggccat cacgaccacc
     2761 gcggctggcg ccgctgagaa ggatgccggc ctgcttccca accccggctt tgataaggag
     2821 atgtcgggtt gggtctttcg ccctggggat ccctcgctgg cggccatcgt ggatgcaggc
     2881 ggcgagcgcg gcaaggtgct cgagctgcac ccggacggga agatcctcgg cgtcgaaacc
     2941 gagaagctgg agcttggcaa ggcgctggag gccgatgccg cctatgaggt gaccgccctc
     3001 ctgaagaacg aggagatcaa gaacggcgtc ttcgctttct ccatgtactg ctacgacgcc
     3061 gccgggaagg cgctgcgcca gattgccttc tacggcctca acaccaaatc ggcgccgcac
     3121 gattggaagc gagtccgggg cgagtttggc aaaggctcgg ccaatccgct tccggaaggc
     3181 acggcgagta tctgcattcg cttctccttc tacgaaaaga gcggcaattg ccaggcaaag
     3241 gttctggtgg atgacgtgat cctgaagaaa catcagccgg ccgtgaaaaa cgactggccg
     3301 gccgaggtag tggccgatgt cggcgacctc caggtgcgct tcgagagccg ctccttctgg
     3361 acgctctacc gaatcgatta ccagggcacc cgcctctgcc atgaccggtg gggttcccac
     3421 tacggcacgg tcgccttctt cccgggcgtt ggcttcatcg gcagcgggca caccgagaat
     3481 gagaacgagg aggttctctc tcgcgcgctg gaggtggatg gcaagccggt cgcccagccc
     3541 gaaaccactt accggtgccg caagatcgtc ctccgcaagg aatcgaaggt tcgcacgctc
     3601 cattataaga cgacgatcac cgtcgctgat aaccgcatct gggaagaggt catcctgaaa
     3661 gcggatgaac cgaccaaggt cgatctcatc taccacttca tgcacccgtg gacgcacacc
     3721 gccaccgagt atctggccga gatgcccgac ggcactcggg tagagggcag cttcacggat
     3781 agcaagaagc tgttgctgga caagcccgcg aagtggtccg ctatctacga tgcccccagc
     3841 ggtaagggag cggtgaccta cgtcctcgaa gtgccggaag gccagccctg gtccaccctc
     3901 tattgggacc agcccgagcg ttaccggaag cactatctga ggacctttgt gaagagcacc
     3961 gttccggcga atgaggagct gcgctaccgc atcgtgaccg ttccgttcgc cgcagcggcg
     4021 gagaagtgga aggaagaagc cgcgaaggtc gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nncctgctcg acctggtgga tgccaacacc gtggatctgc cggtggcctc
     4201 gcttctggcg ggcgcggtgg catcgggcgc atccctgctc accgcggccc ggcctggcaa
     4261 cgccgggaag acgacggtgc tggccgcgct tctctccttc atcccgccgg gcaggcgcat
     4321 tgtcaccgtg ccagatggcc cgccccggag cgtgccgccg gacacgtgcc ttctggcgca
     4381 cgagatcggc tccggtccct ggtatgccta cctttgggga ggcgcggcgc gaggcttttt
     4441 cgaggccgca gccgcccccg gcgtacaggc agcctcgtgc atccacgccg acacgctaga
     4501 agagctacgg atgacgctgt gcgggccatc catcggcctg ggagagaagg agttcgccgc
     4561 gattgacctg gtggcgttcc tgcgcctcga ccgcaccctg acgggctacc ggcgccgcgt
     4621 ctgtgccgtc tatgcggcca ccggcacccc gggcgccatg agccaccaac agatctgccg
     4681 ttggaacgag gcgaccgatt ctttcaccgt ggacgccgtg ccagagcggt gggcggctgt
     4741 ctcggagcgc gcggctgagt tcctcgaagc cgcgcgcacg gccggctgca cccgcttcga
     4801 agagctgttc gcgcgctggg ccaccacctg accgctagag tttcgggggc ggaggcggag
     4861 cggaatcgct acggacggtg agcgggctga tcgacacggc ctggctggcg attcccctga
     4921 tatccgcggc gctaatcgac gcataatagc gttgatcggc actgctgagg ttagcgggca
     4981 cctggatcga gacctcgtag tagcccgcct ggttccaggt catgtcatgg accgcctgcg
     5041 acccatccgg gcgggtgagc gttgcccgga cccacgccac gccggaggca tcggtcacca
     5101 gggcgatgat ccgcgccgtg cccccgatat cgcccaacga ggatggctcg aaccgcgccc
     5161 agacgatccg gggcgggtcg tagtctgcct tctgctcatc gccccctccc ccgccacatc
     5221 cggcgagcag ggctccgatg agaacggcca cgcaccgcag gcatggccat atgccggcgc
     5281 gccgcgcgcg cacctgaagg gaatgcttgc tcatagagat tcaggggtgc aggagaaccc
     5341 cgtcgctctc ccccacctgc ggggagggcg cgcatggaga atccgtgcag ctcccgtcct
     5401 cctctcatgg tgctcgacgg gatccctctc gtacaaaggc acttccagcc ttcccgagtg
     5461 gaatagccgc cgcgagggca gcccgcaatt ggggagggcg cagcccccgc tccaggagga
     5521 tcattcggct ttcccgacgc catactcctg caacgtctcc cacatcgcca ggatgttctc
     5581 tgggggcaca tcggcctgga tgttgtggac ggtgttgaag acgaaaccgc cgccgggagc
     5641 gaggtcatcg atgcgccggc gcacctcatc gcgaacatcc tgcggcgtgc catgtggcag
     5701 aatacgctgg gtatccactc caccgcccca gaagacgata tcgcgcccga attccgcctt
     5761 gagccgtcgg gtgtccatgc cagccgcgct cacctgcacc gggttgagga tatccacgcc
     5821 cacctcgatc aggtccggga gaatctcgac gacactacca caggtgtggt agaagacgaa
     5881 gacgggcgcc tgcttcttga tgtgggcgaa gagctgcgcc tggcggggct tcaccacctc
     5941 gcggtagacg cgcgggttca tcagcaggca ctgctgcatc cccaggtcgt ccccctccat
     6001 cgcgatatcc accaggtcgc ccacttcggc cagggcgcgc tcccaatagc gcagcttcgt
     6061 ttccaggatg agatcgagca tcgcttcggc catcggccgg tcggcaacga gatcggtgaa
     6121 gaagcgctcg aagccacgga gccagaggca catctcggta agccccgcgc agacgccacc
     6181 gaggatcacg gcgcacccgg tgtcgcgcgc cacctgggcg cgcgcacgca ggcccgcgaa
     6241 ccgcgccggg tcctgcgggt ccggccaggg atagcgccgg atctcctcgg gcgacgtggc
     6301 ctcggcgagg gggtgaccgc aaagatcgta ataccagccg tcgcggggca tgcggcgggg
     6361 caatccccag gcgtcggtga aggtgcggta gcggccctcc tcccggatgc acagtctcca
     6421 gccggaaggg tcgccactgg ccacgccgcg cgtatcgacg cgcagggcct gaaggaggtc
     6481 ttcatcgaca gcggcaagct gttgggtaag gtcgctgatt gccgcttcgc gcggcgccag
     6541 gccgagagcc ggacgcaggc gctcataggc aatgcggtgg attccggtca ccttggtaga
     6601 gcccagatca aacggtacgc gatccggctc ctggtgccgc agggtagtca ggattcgctc
     6661 gcgggatgtc atggcgtcat ctcctggggg cgggtgcgct gggatgggcg agtgatggcg
     6721 taactgcgga gcgcgtcgag gccaatgcgg cgaagcacag cgccaaaggt ctcgcggggc
     6781 cgagctcgct cgctcacaaa gccgatcacg cgcgcggcca cctcgggggc atcctcctca
     6841 gggacaaagc gcgcgagctc cgcccccagg caaggccgac ggcctgcctt cccgcccacg
     6901 taaaccgtat agccgacgcg agccgggcgc aacgcattga aaccacacgc cgctacacaa
     6961 tcgccgcaga agttgcaccg ctgggggtcg cgccgtggca ggccgcggcg catgatgagc
     7021 gccccctcct tgcacgcctc gacgcacgtg ccattcccct ggcactcccc ccggacgagg
     7081 atcggctcga cgatgccaac aaacccgatg tcggcggtct gggggcagct acacgcttcg
     7141 gcacagcccg agatcgcgac acgcacgggg atcggg
//
LOCUS       DAOSCV010000081         7149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6721_length_7149_cov_3.583874, whole genome shotgun sequence.
ACCESSION   DAOSCV010000081 DAOSCV010000000
VERSION     DAOSCV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6721_length_7149_cov_3.583874"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1312
                     /locus_tag="PLY56_03270"
     CDS             53..1312
                     /locus_tag="PLY56_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ20530.1"
                     /translation="MQEERIARTITRPAREVPVMIETDVLVCGGGPAGTAAAVAAARQ
                     GASVALIERYNHLGGLATGGLVLVLPHFVDHGRQVIGGFGLETRSALLEHGRAGMRKH
                     GDDTSAFDPEALKWLSIRQCRDAGVEVLHHCWLADVVLREERVAGVIFESKSGSGAAL
                     AKVVVDATGDGDIFAWAGAEFEHSNQGIGLPFRLAGIDVPRWQADRAERHQWHVDFWK
                     RLREEIGWSSPFYIEHLPPQPDMAWGNNMHSVADGLDVRALSQIDMDARLKIHQAVER
                     IRAELPGFENVWLVETASQTGVRRTRRLAGTFRLTAAEVSQFDYRHEEAIGRGNDHRR
                     RGPVYDIPYGCLVPKSLDGLLACGRCVSTDDEALEAMREIHVCWVTGEAAGVAAALAA
                     KRGCQPREVPIGVLQQVLRESNVAFAD"
     gene            1461..1934
                     /locus_tag="PLY56_03275"
     CDS             1461..1934
                     /locus_tag="PLY56_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20531.1"
                     /translation="MATRVTKKEQAVELHDQGLSVEEIASRLKTSPTYVANTLIESGR
                     TPEYFDLYTHTGPQNPYARRLAGVLRFRTPEAARASILHLDEIFHEYEAQGDLRGQHQ
                     VQLLALVGRNRAEGIGKLEAADLFTDWLLAHLMPRRRMRRRREERESRTSPAPGH"
     gene            complement(2052..3593)
                     /locus_tag="PLY56_03280"
     CDS             complement(2052..3593)
                     /locus_tag="PLY56_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20532.1"
                     /translation="MHRKLRSLWLGITGRIMLTVCLLLAPYVGAHGADGDRVHAHIPG
                     LVPVAVEASRITRAPSGKIHATGGVVISRGDDTLHASEATYDPATDEIEAVGDVRLFQ
                     PGRRLTSERLRYGARTGEILGTDIRAALASKAPRVEYHFQGESLSGSPEEYHLHGGLF
                     TTCEQHPPHFGLRAREITYIPGDRVRARRVSLLFLGTPIMTLPSYTLDLTDDRDKPGL
                     FPQLVLGATDGIGVRGDLRLPMPDRGGLHAYAILSARHTLRGGLGIYAGESIPLGIRA
                     GFKEAAPTRFASGLTVSVLPAITFHLPELSSPSPALQLGMARYQPTFDTLANDVTGGG
                     WSGDKRYRLLFAGSAGRYTEHPTDVSDNRLNLTGAFEVRPFQAFEKIELGAALRGRLA
                     YYGGGSHYGVVSPEINAQWSPSKRHLLRAEFRHQIARGSTPFLFDRIDAERNLNLTWR
                     TRSAMDAIELAADIDLDNWSLYNWSVGYSRRVDCLQPGLTLHRRGEDWGIGVTLEIPG
                     LTGGF"
     gene            complement(3811..6120)
                     /locus_tag="PLY56_03285"
     CDS             complement(3811..6120)
                     /locus_tag="PLY56_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOJ20533.1"
                     /translation="MMKKLAILGIAATVMATTVSLPRAHAQAEAFNPQGVLKPEPFAD
                     VPPDHWAHSAVEQLRQAGLTEGYPDRTFKGKRPMTRYEFARFVQLLLQSLQPRFASLS
                     QLEQQVREMGTREVPPAGLTQQQLNDAIAELRSQILTREQVQQIVRDMLAQQPQPTGG
                     VTPEQLQTLQRLVDEFRDQLARLGVDVDAVKKELADLRGRVETIEAQLARMPKITGDL
                     NLIGKGSWKSDQVLQAGVFRPVFDHDGRPLNDTDNVLEPVLPYYDLDLGITAMPTSNV
                     RVAAILNVGNYVSSYRDPVFHLYDRAGTDVVTPVHAYMTVPFNFLGANEITVGRQPTQ
                     LTSYTFKAIDPDTYTFIPREDSGDVIFWGVNTTWRMGSAKLRAFAGVNPVDGRLEPLN
                     VYPAGLFGTGTPGVFGAFTTAGSVPIVDIGNFAGARLSFGEPETGVPADLLAREVSVA
                     PASLRTAATERPAPAAEPGPGVDYGYSYGPIRNAIFGINWLMAGGDYRATPLVVDGTP
                     VTEDARAQVVSADARFKLGRLLFNGEFAFGESQTGRLPRPRSLDDTAIDLRAQYNIGL
                     GFGDLGIGGGWRRIELGYNVPGYWGYLGSWKNPRGIEGWMAQAELPLRRNVASFLRNI
                     TLRGSAEWYEGTQNVPTAGAPAGLDPSEENLRIDRYQAGIAFNLTDRNPVTVGFEQVF
                     RNYVAANVNDAPLALPTMGVGGSPLVGTRIKNTETYINFGIGHNFTEDINLALVYQLV
                     DFGKYDVDVSNQPANYRGYVASAQVGVRF"
     gene            complement(6628..7056)
                     /locus_tag="PLY56_03290"
     CDS             complement(6628..7056)
                     /locus_tag="PLY56_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20534.1"
                     /translation="MPLIMRWLHIGAAITSVGGLFLLVSVILPSLRAADPEQATLLLR
                     GALRRLWPALWILGAALLLSGFYAAALVRPSMTPTYHLVLGIKLLLSLAFLGTALVAI
                     RPASDAPERLARRLDWARLALIFGVAAVLLSSYLWHLRVG"
BASE COUNT         1244 a         2231 c         2529 g         1145 t
ORIGIN      
        1 aggtgagccc gcgcgcaggc gagaatcacc aatgtgaaga gggggtgcag gcatgcaaga
       61 ggagaggatt gccaggacaa tcacccgccc cgcgcgggaa gtgccggtga tgatcgagac
      121 cgatgtgctc gtctgcggag gcgggccggc agggacggcg gccgccgttg ccgccgcgcg
      181 ccagggtgcc agtgtggcgc taatcgagcg ctacaaccac ctgggcgggt tggcgacggg
      241 cgggctagtg ctggtgctgc cgcattttgt ggaccacggg cgccaggtga tcgggggctt
      301 tggcctggaa acgcgctccg cgctgctgga gcacgggcgt gcgggcatgc gcaagcatgg
      361 tgatgatacg tccgcgttcg atccggaggc gctgaagtgg ctgagcatcc ggcaatgccg
      421 cgacgccggg gtcgaggtgc tgcatcactg ttggctcgcc gatgtggtcc ttcgcgagga
      481 gcgcgtggcc ggcgtcatct tcgagagcaa gtccggctcc ggcgcggcac tggcgaaggt
      541 ggtggtcgac gcgacgggcg atggcgacat ctttgcctgg gccggcgcgg agttcgagca
      601 ttcgaaccag gggatcggcc tgccgttccg cctggccggg atcgatgtgc cgcgctggca
      661 ggcagaccgc gccgagcggc accagtggca tgttgacttc tggaagcgcc tgcgcgagga
      721 gatcggctgg tcgagcccgt tctatatcga gcatctcccg ccgcaaccgg atatggcctg
      781 gggcaacaac atgcacagcg tcgcggatgg ccttgacgtt cgcgcgctct cgcagattga
      841 catggacgcg cgtctgaaga tccatcaggc cgtggagcgg attcgcgcgg agctgcccgg
      901 cttcgaaaat gtttggctgg tggagaccgc gagtcagact ggcgtgcggc gcacgcggcg
      961 gctcgccggc accttccggc tgacggcggc ggaggtgtcg cagttcgact atcggcacga
     1021 agaggcgatt ggccggggca acgaccatcg ccgccggggg ccggtgtatg atattcccta
     1081 cggctgcctc gtgccgaaga gtctcgatgg tcttctggcc tgcgggcgct gtgtctccac
     1141 cgatgacgag gcgttggagg cgatgcgcga aatccacgtc tgctgggtga cgggcgaggc
     1201 cgccggtgtg gccgccgcgc tcgccgcgaa gcgaggctgc caaccgcgcg aggtgccgat
     1261 tggcgtcttg cagcaggtgt tgcgcgagag caacgtggcg ttcgccgact agcactcaaa
     1321 ggcgtttgtg aaaccacttc accggccgcc cgactgggcg gccggccgcg tgtacgtgcc
     1381 cgaatcggac cgcccggccg tgccccaccg gtttcggggc gggtcggtcc ggataggatc
     1441 gccagggaag ggagaatccc atggcgaccc gggtgacgaa gaaggagcag gcggtcgagc
     1501 tgcacgacca ggggctctcc gtcgaggaga tcgcgtcgcg cctgaagacg tccccgacgt
     1561 acgtggccaa cacgctgatc gagagcggtc gaacgcccga gtattttgac ctctacacgc
     1621 acaccggccc gcagaacccg tatgcgcgcc ggctcgcggg cgtgcttcgc ttccgcactc
     1681 ccgaggcggc acgcgcgagc atcttacatc tggatgagat cttccacgag tatgaggccc
     1741 agggcgatct ccgcgggcag caccaggtgc aacttctcgc gcttgtcgga cgcaaccgcg
     1801 ccgaggggat cggcaaattg gaggcggcgg atcttttcac cgactggcta ctcgctcacc
     1861 tgatgccgcg ccggcgaatg cgccgtcggc gtgaagagcg cgagagccgc acatcacccg
     1921 ctcccggcca ttagcggcgc gtgctgcgcc gctggcttgc cacgcttttc aaaaccgctc
     1981 ctgccccgcg cgtgggtgtc ggggatgccc atcccctcga tcccggccag tcaggcggtt
     2041 cgcccggccg atcagaatcc tccggtgagg cctgggatct ccagtgtgac gccgatcccc
     2101 caatcctcac cgcgccgatg gagcgtcaag ccaggttgga ggcagtccac gcggcgcgag
     2161 taacccacgc tccagttgta gagactccag ttgtcgaggt cgatatccgc cgcgagctcg
     2221 atggcgtcca tagccgagcg ggtgcgccag gtcaggttga gattccgctc ggcgtcgatt
     2281 cggtcgaaga ggaacggcgt ggagccacgg gcgatctgat gtcggaactc ggcgcgcagc
     2341 aagtgccgct tcgaggggct ccactgcgca ttgatctcgg gagagacgac gccatagtgc
     2401 gagcccccgc catagtaggc gagccggccc cggagcgccg cgcccagttc gatcttttcg
     2461 aaggcctgga aggggcggac ttcaaaggcg cccgtcaggt tcaggcggtt atcggaaacg
     2521 tccgtaggat gctcggtgta ccgccccgcg cttccggcga atagcagacg atatcgcttg
     2581 tccccactcc agccgccccc ggtgacatcg ttagcgagcg tgtcgaaggt gggctgatac
     2641 cgggccattc ccagttggag cgccggcgag ggcgaggaga gctccggcag gtggaacgtg
     2701 atggccggga ggacggagac tgtgaggcca ctggcgaagc gggtcggcgc ggcttccttg
     2761 aagccggcgc gaatccccag ggggatcgac tcgcctgcgt agatcccgag gccaccgcgg
     2821 agagtgtgcc gggcggagag gatcgcatag gcatggaggc cgccgcggtc cggcatgggg
     2881 aggcggagat cgccgcgcac cccgatacca tcggtggcgc cgaggacgag ctgcggaaag
     2941 aggccaggct tatcccggtc gtcagtgaga tccagggtgt agctgggcag ggtcatgatc
     3001 ggcgtgccaa ggaagagaag cgagacccga cgcgcgcgga cccggtcgcc cgggatgtag
     3061 gtgatctctc gggcgcgcag cccgaagtgc ggggggtgct gttcgcacgt ggtgaagagg
     3121 ccgccatgga gatggtattc ctcgggagag ccagagagcg attcgccctg gaagtggtac
     3181 tctacccgag gcgccttgga tgcaagcgcg gcgcgaatat cggtgcccag gatctcgccg
     3241 gtccgggcac catagcgcag gcgctcgctc gtaagccggc ggcccggctg gaagaggcgg
     3301 acatcgccaa ccgcctcgat ctcatccgtg gcgggatcgt aggtggcttc gctggcatgc
     3361 agggtgtcat caccgcgcga gatcaccacg ccaccggtag cgtggatctt tccggaaggg
     3421 gcccgggtga tccgactcgc ctccacggcc acgggcacca gcccgggaat gtgcgcatgg
     3481 acgcggtcgc catccgcgcc gtgtgcgccg acatagggcg cgaggagcag gcagacggtg
     3541 agcatgatcc gcccggtgat gccaagccaa agagagcgca gcttcctgtg caaacgagtc
     3601 atgccttgtt attgacctcg gaggcaaaca gcgcgcgggt cgccgccgga acgggatcgc
     3661 cgggcgggcc gcacttcgcg gacgggcaaa acggcctgtg tcaatttagg gacgcgccct
     3721 ggcgagataa gttcaagaga gacacaaaag agcggccccc gggggccgaa gaggcctgcc
     3781 cgggggccgc tttgcgtgga aggtgctcgc ttagaagcgg acacccacct gagcgctggc
     3841 gacataaccg cggtagttcg ccggctggtt ggagacgtcg acatcatact tgccgaagtc
     3901 caccagctgg taaaccagag cgaggttgat atcctccgtg aagttgtggc cgatgccgaa
     3961 gttgatgtag gtctccgtat tcttgatgcg ggtacctacc aacgggctgc cacccacgcc
     4021 catggtgggc agggcgagcg gcgcgtcgtt cacgttcgcg gccacgtagt tgcggaagac
     4081 ctgctcgaag cccaccgtga ccgggttgcg gtcggtgagg ttgaacgcga ttcccgcctg
     4141 atagcggtcg atgcgcaggt tctcctccga ggggtcgagt ccggcaggag cgccggccgt
     4201 cggcacgttc tgggtgccct cgtaccactc tgcgctaccg cgcagggtga tgttgcgcag
     4261 gaaggaggcc acgttgcggc ggagcggcag ctcagcctga gccatccagc cctcgatacc
     4321 gcgcgggttc ttccagctgc ccagatagcc ccagtagccg ggcacgttgt agccgagctc
     4381 gatgcggcgc cagccaccac cgatgcccag gtcaccgaag ccgagcccga tgttgtactg
     4441 ggcgcgcagg tcgatggcgg tgtcatccag cgagcggggg cgcgggaggc ggcccgtctg
     4501 cgactcgccg aaggcgaact cgccgttgaa caggaggcgg cccagcttga agcgggcgtc
     4561 ggcgctcacg acctgagcgc gcgcatcctc ggtcacgggc gtgccgtcca cgacgagcgg
     4621 agtcgcgcgg tagtcaccac cggccatcag ccagttgatg ccgaagatgg cgttgcggat
     4681 cgggccgtag ctatagccgt agtccacgcc agggccaggc tctgccgcgg gcgcggggcg
     4741 ctcggtagca gcggtgcgca gcgacgcagg cgcaaccgag acctcgcgag cgagcagatc
     4801 ggcgggaacg ccggtctcag gctcgccgaa ggagaggcga gcacccgcga agttgccgat
     4861 gtccacgatg ggcaccgagc cagccgtggt gaacgcgccg aagacgccgg gcgtgccggt
     4921 gccgaagagg ccggcggggt agacgttcag cggctcgagg cggccgtcaa caggattcac
     4981 accggcgaag gcgcgcagct tggccgagcc catgcgccag gtggtgttca cgccccagaa
     5041 gatcacgtcg ccgctatcct cgcgggggat gaacgtatag gtgtccgggt cgatcgcctt
     5101 gaaggtgtag gacgtcagtt gggtcggctg gcgaccaacg gtgatctcat tcgcgcccag
     5161 gaagttgaag ggcacggtca tgtaggcgtg gaccggggtg accacatcgg tgcccgcgcg
     5221 gtcatacagg tggaagaccg ggtcccgata gctcgagacg tagttgccga cgttgaggat
     5281 cgcggcaacg cgcacgttcg aggtcggcat ggcggtaatg cccaggtcca ggtcatagta
     5341 gggcagcacc ggctcgagaa cgttgtcggt gtcgttcagc gggcggccgt cgtgatcgaa
     5401 gaccgggcgg aagacgccgg cctggaggac ctggtcgctc ttccaagagc ccttgccgat
     5461 caggttcaga tcacccgtga tcttcggcat gcgcgccagc tgggcctcga tcgtctcgac
     5521 gcgcccacgc aggtcggcca gctccttctt cacggcatcg acatcgacac cgaggcgagc
     5581 gagctgatca cggaactcat cgaccaggcg ctggagcgtc tggagctgct caggcgtgac
     5641 gccgccggtc ggctgcggct gctgcgccag catatcgcgc acgatctgct ggacctgctc
     5701 gcgggtgagt atctgcgagc gcagttccgc gatggcatcg ttgagctgct gctgagtgag
     5761 gccggcagga ggcacttctc gcgtgcccat ctcccgcacc tgctgctcga gctggctgag
     5821 tgaggcgaac cgtggctgca ggctttgcag caggagctgc acgaagcgtg caaactcgta
     5881 gcgagtcatc ggccgcttgc ccttgaaggt cctgtcggga taaccctccg tcagacccgc
     5941 ctgcctcagc tgctccaccg cgctgtgcgc ccagtgatcg ggcggcacat ccgcgaaggg
     6001 ctctggcttc aacacgcctt gcgggttgaa cgcctcagcc tgggcatgcg ctctgggcaa
     6061 ggagaccgtg gtcgccatga cggtcgctgc gattcccaaa atcgcaagct tcttcatcat
     6121 ccttcatcct ccttgcagtt gcaactgtcg ctgtccacgc cgcaagggcg tggttcctac
     6181 aggctccccg atcgggcttg ccagaagggg actccggatg agagttgccg cgtttccttg
     6241 ccatccgggc cttttctggc ctgccacctc gggagtactg caccatcgta tcaggccgcg
     6301 cgaggaacat cggaagcgca accgctcccg ttccgcacga ggcccgcacg ctggcggcac
     6361 ctcctgattg cggcgcttta cagcggtccc ggtcagcgtt caagtcaacg tatgcgggtg
     6421 cgccgaccgc ctggaggcct gcccccaggc cctaccctgc aaccgggcag ggaacgaacg
     6481 ccaggtagtg cttctgagta tagcacacag gcaggtgcgt gtcaagcagg catgctccgg
     6541 tcacggttgc ccccagggcg cctgctttcc actatacttt gacaccattg ccgtcggaaa
     6601 gttgcggcgc gcgaaacagg cgcgccccta ccccacgcgc aggtgccaca ggtagctcga
     6661 gagaagaacg gcggccacgc cgaagatcag cgccagccga gcccaatcga ggcgtcgcgc
     6721 cagccgctcc ggggcatccg aagccgggcg aatggcaacg agcgccgtgc caagaaacgc
     6781 gagactgagg agcagcttga tccccagcac gaggtggtag gtcggagtca tcgaaggccg
     6841 cacgagcgcg gcggcgtaga acccggagag gagtagcgcc gcccccaaga tccagagggc
     6901 aggccaaagc cggcggagcg caccccgtag caggagcgtg gcctgctccg gatccgctgc
     6961 gcgtagtgat gggagaatca cgctcaccag aagaaagagt ccgcccacgc tggtgatcgc
     7021 cgcaccgata tggagccagc gcatgatcaa gggcatcgcg gtcccctctc gcctgttgtg
     7081 tgcgccattg tagcatacgc ggtggagggc gtcaagcacg gtcgaggagg aaatcgcgtc
     7141 aggattctc
//
LOCUS       DAOSCV010000082         7090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6794_length_7090_cov_3.378252, whole genome shotgun sequence.
ACCESSION   DAOSCV010000082 DAOSCV010000000
VERSION     DAOSCV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6794_length_7090_cov_3.378252"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PLY56_03295"
     CDS             complement(<1..1765)
                     /locus_tag="PLY56_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20535.1"
                     /translation="MANETAAERARQFLAEATQFRLGKLLTESPHPRSAQLSQRAGES
                     IPAGLEVLFDIDRDVVETYRQWVESDAPGRIRDEVLAALRAGGRLFFTGCGATGRLSI
                     QLDSIWRDFWQRRRASGLPGVDEWEDRTFSVMAGGDFALIKSVEGFEDYTQFGRRQIR
                     DRGVRAGDVVFAITEGGETSFVIGTAWEALDAGARVFFVYNNPDDVLRENVERSREVI
                     DEPRITKINLTTGPMAITGSTRMQATSIQLCVMMTILEMVLRALTGADSQEDVPGATL
                     RALEEMHATLASEALRDDLAKLVAREESVYRARHRNTYFADRLGIDVLTDTTERSPTF
                     CVPSFRKFDDPDAAESWSFLFLPPYDTAAAWEHLLKRTLAPVEWSLEEVREMLPAGEA
                     ERQHAIVRGISREEILRFRIGLDGLPYRAIGPGDAATAILTEEEKEALFTPDGFYRVR
                     LEQADASGAATAVLFFGRPGAIEEVRAFLATWEVSALPILVPVPETDLLLNLPLRVGA
                     KMLLNALSTCTMVRLGRVLGNVMIWVVPSNLKLIDRSTRYIANLTGLSYEEACYLLFE
                     AMEYVGPRWHAGKEYPAPVGLS"
     gene            2036..2677
                     /locus_tag="PLY56_03300"
     CDS             2036..2677
                     /locus_tag="PLY56_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20536.1"
                     /translation="MRRDEVRGFTLIELLVVIAIIAILAAILFPVFARARENARKSNC
                     LSNLKQLGTAHMMYCQDYDEMTASYQDVPAAAATRSWMNMFEPYMKNTGVLNCPSAPG
                     KFRVSSSGMYVSYYGVNITNVSVSGATWTYLFCYRPLAQIENPAETLMIADLDTEGVY
                     LRWNSVENRANNVATRHSEGTCCAFFDGHAKWVKADVLLSPSSLPTLWKGGLP"
     gene            2808..4007
                     /locus_tag="PLY56_03305"
     CDS             2808..4007
                     /locus_tag="PLY56_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HOJ20537.1"
                     /translation="MMERQPLSRSVLILAVALAGALLAGCRGGGGITTGSQAAPAARP
                     GENAPARSGAEALPVEERLRAPEGFRVEQVAQGLQGVRLLAFSPDGRLFATQPRSGRV
                     TLVPIGGGAPTAWATGLNRPHGIAFHDGFLYVAETDAVVRWRYQPGAQRAPGAPERVA
                     SLPGGGSHWTRTLRFGPDGKMYVSIGSTCNVCIEDDPRRAAILQFAPDGSGERIFASG
                     LRNTVGFDWDAQGRLWGVDNGTDNMGDNFPPEELNLIQDGGFYGWPYAHGDCVPDPRY
                     GSRRPEMVERCIPPAFKITAHSAPLGIIFYQAEQFPEAYRGDAFITLHGSWNRSAPSG
                     YKVIRIHFSNGQPVAMSDFITGFGQGRGAWARPVGIVTGPDGSLYLSDDRAGSIWRVR
                     YTGSSGS"
     gene            4291..6780
                     /locus_tag="PLY56_03310"
     CDS             4291..6780
                     /locus_tag="PLY56_03310"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOJ20538.1"
                     /translation="MERRILLVWVAVLALLVPAERANAAELLFGCETDAEVSAWMLRS
                     GGQDRLARDPGYATQGEFSMRFATPAWKDGMAQWPAFEVEPPVSDWRGYDRLMIDITH
                     PGDGEHTLSLFVSDRKVPFREGLRYRFDLPKRGYRRFIVPLNFPEKVNRGDIGILHFF
                     TQRPPSEMVLYIDNITLLRPGEQPPEPRVGFAAQVAKLFLDSLGGLDAALEQSRASLL
                     RIADTPALRQRAREEMDALAACARRAREEASSPTLTLARLETIRHELEGLPGEARRVE
                     SIFRFRKAFEGLRLGDTPLLVGFATSMEKLLPRRMPFELSVARRVELALARNERESLQ
                     VAVMPRDGALKGVRVSVTDLKSRSGAVLPAARINCDVVGYVETKARPPYEVSHVGWWP
                     DPILDFLGPVDVASGDLQSFWVRVHATKDQAPGLYEGTLQVAAEGVEPLSFPLAVRVY
                     NFTLPVHTPLPTAITFNELKSQMGGAERWAEMKHRWADFLADYYIDYDNLYRQGPPDW
                     EIAKRLHDEGRLVAFNLGNVFNAGTKEDGFDEAIAATIARLRPAYEKAKELGVLDHAY
                     IYGFDERPKEQFPLLERSARALRQAFPEVLLMTTSYDHSYGLDSEVKTIDAWCPLTPR
                     FDPERVKRARAAGKKVWWYICCNPHRPYANWFVESDAIEARLLMGAMTAKYRPDGFLY
                     YALAKWNNNKPIETGPFTEWNPVSYVTYHGDGSILCSGPGGKPVPTIRLENYRDGMED
                     FAYACILEECIRRYEAKGERLTAREREWLAKAREALPVPETLVNTVAEYSRDPKAVYA
                     WRNRVGEMIDRSGVPDPNPWGKAFGVRGFGG"
     gene            6784..>7090
                     /locus_tag="PLY56_03315"
     CDS             6784..>7090
                     /locus_tag="PLY56_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ20539.1"
                     /translation="MASERPNLLLICADQWRGDSLGVAGHPVVETPHLDALARSGTLF
                     TQAYSACPSCIAARAALFTGLSPRRHGFVGYRDGVHWRYPVTLAGTLAAAGYHTQCIG
                     "
BASE COUNT         1166 a         2297 c         2420 g         1207 t
ORIGIN      
        1 ccgagagacc cacgggggcg ggatactcct tgccggcgtg ccagcggggc ccgacgtact
       61 ccatcgcctc gaagaggagg taacacgcct cctcgtagga gagcccggta agattggcga
      121 tgtagcgcgt cgagcggtcg attagcttca ggttgctcgg cacgacccag atcatcacgt
      181 tgccgagaac gcggcccagg cggaccatgg tgcaggtgga gagcgcgttc aggagcatct
      241 tggcccccac gcgcaacggc aggttcagga gcagatccgt ctctggaacg ggaaccaaga
      301 tggggagtgc cgacacctcc caggtggcga gaaaggcgcg cacctcctcg atggcgcccg
      361 gccgtccaaa gaagaggacc gccgtggcgg ccccgctggc gtccgcctgc tccagacgca
      421 cgcgatagaa gccgtcgggg gtgaagagcg cctctttctc ctcttcggtc aggatcgccg
      481 tcgcggcatc gcccggcccg atggcgcggt aggggagacc gtccaggccg atccggaagc
      541 ggaggatctc ctcgcgcgag atgccgcgca cgatggcgtg ctgtcgctcc gcctcgcctg
      601 ccgggagcat ctcgcgcacc tcctccagag accattccac cggggcgagc gtgcgcttca
      661 gcaggtgctc ccatgccgcg gcggtgtcgt acggcggaag gaagaggaac gaccatgact
      721 ccgcggcgtc cggatcatcg aacttccgga agctgggaac gcagaaggtg ggcgagcgct
      781 cggtggtatc cgtgaggaca tcgatcccga ggcggtctgc gaagtaggtg ttccgatggc
      841 gcgcgcggta gacgctctcc tcgcgcgcga cgagctttgc caggtcatcg cgcagggcct
      901 cggaagcgag ggttgcgtgc atctcctcca gggcgcgaag cgttgcgccg ggcacgtcct
      961 cctgtgagtc agcgcctgtg agggcgcgca ggaccatctc caggatcgtc atcatcacac
     1021 acagctggat gctggtggcc tgcatgcgcg tggagccggt gatcgccatc gggccggtgg
     1081 tcaggttgat cttggtgatc cgcggctcgt caatcacctc gcggctgcgc tctacgttct
     1141 cgcggaggac gtcgtcgggg ttgttgtaga cgaagaagac gcgcgcaccg gcatcgaggg
     1201 cctcccaggc ggtgccgatc acgaaggatg tctcgccccc ctcggtgatc gcgaagacga
     1261 catcgccggc gcgcacgcca cggtcgcgaa tctggcgccg gccgaactgg gtgtaatcct
     1321 cgaagccctc caccgacttg atgagcgcga agtcgccgcc cgccatgacg ctgaaagtgc
     1381 ggtcctccca ctcgtccacg ccgggcaggc ccgaggcgcg ccggcgctgc cagaagtcgc
     1441 gccagatcga atcgagctgg atgctgaggc gcccggtggc gccgcaaccg gtgaagaaga
     1501 gccgtccgcc cgcgcggagc gcggcgagga cctcgtcgcg gatgcgcccg ggggcgtcgc
     1561 tttcgaccca ctgccggtag gtctcgacga catcccggtc tatatcgaac agcacctcca
     1621 gcccggcggg gatgctctcg ccggcccgtt ggctgagctg ggcggagcgc gggtgcggcg
     1681 actcggtgag cagcttcccc aggcggaact gggtggcttc cgcgaggaac tgcctggcgc
     1741 gttcggcggc ggtctcattg gccattggat cacctcctgg ccccgtggat ggggtatcct
     1801 catggtacca gacgtagggc agatgtcaag agccagatcc ggagcgcctc tctttgcaaa
     1861 gcacgtgaag gaaccgttcg atccagcggg aaatatcagg tggaagggtg gccgcgctgc
     1921 ccggggcctt gctccctgtg ccccaccgcc atgccaggcg tgccgaagga gccccacgct
     1981 tgcgtgcgct tcgatggtgg ggatggatcg ccgcggccaa agggagacag tcaaaatgcg
     2041 tcgagatgaa gtacgtggtt tcacactgat cgagttgctt gtcgtcatag ctatcatcgc
     2101 catccttgcg gcgattctct tcccggtgtt tgcccgggcg cgtgagaacg cgcgcaagtc
     2161 gaactgcttg agcaacctga agcagctcgg gacggcacac atgatgtact gccaggacta
     2221 cgatgagatg acggcgtcgt atcaggacgt gccagcggcc gcggctaccc ggtcgtggat
     2281 gaacatgttc gagccgtaca tgaagaatac gggcgtcttg aactgcccga gcgcgccggg
     2341 caagtttcgc gtcagcagct ccggcatgta cgtcagttac tacggcgtga acatcacgaa
     2401 cgtcagcgtc agcggcgcta cctggacgta tctcttctgc tatcgcccgc tggcgcagat
     2461 tgagaatccc gcagagacgc tgatgatagc ggacctcgat acagaggggg tctacctgcg
     2521 ctggaactcg gtggaaaacc gggcaaataa cgtggcaacg cgccactcgg agggcacctg
     2581 ctgcgccttc tttgacgggc acgccaagtg ggtcaaggcg gacgtgctgc tgtcgcccag
     2641 ctcgcttccg acgctttgga agggtgggct cccgtagctt tccagcaggc tacctgatgt
     2701 ggggccgggt gctgtctcct ggggggcagc gcccggccct tcttgcgtta accgttcgcc
     2761 cttcctggtt tcctcagccg caacaacggg aaagctgtga cggggggatg atggagcgtc
     2821 aaccgctgtc acggagtgtc ctcattctgg ctgtggcgct ggccggagct ctccttgccg
     2881 ggtgtcgcgg gggcgggggg ataacgaccg gctcccaggc ggcgcctgcc gcgcgcccgg
     2941 gtgagaacgc gccggcgcgg agtggggcag aggcgttgcc agtggaagag cgcctccggg
     3001 cgccggaggg cttccgcgtc gagcaagtcg cccaggggct tcagggcgtg cgcctgctcg
     3061 cgttcagccc cgatggtcgc ctctttgcca cccagccccg atccggccgg gtgactctcg
     3121 tgccgattgg cgggggcgcg cccaccgcgt gggcaacggg cctcaaccgg cctcatggga
     3181 tcgccttcca cgatggtttc ctctatgtgg ctgagacgga tgccgtcgtg cggtggaggt
     3241 atcagccagg ggcacagcgg gcgcccgggg cgccggagcg cgtcgccagc ctgcctggcg
     3301 ggggcagcca ctggacgcgc accctccgct tcgggccgga cggcaagatg tacgtctcca
     3361 tcggctccac ctgcaacgtc tgcatcgagg acgacccgag gcgcgcggcc atcctgcaat
     3421 tcgcacctga tgggagcggc gagcggatct ttgccagcgg gttgcgcaac acggtgggct
     3481 tcgattggga tgcccagggc cggctctggg gcgtggacaa cggcaccgac aacatgggcg
     3541 acaacttccc gcccgaggag ctgaacctca ttcaggatgg cggcttctac gggtggccgt
     3601 atgcccacgg cgattgcgta cccgacccgc ggtacggtag ccgccgccct gagatggtgg
     3661 agaggtgcat tcccccggca ttcaagatca cggcgcactc ggcgccgctt gggatcatct
     3721 tctatcaggc ggagcagttt ccagaggcat accgcggcga tgcctttatc accctccacg
     3781 gctcgtggaa ccggagcgcc cccagcggct acaaagtgat ccgcatccac ttctcgaatg
     3841 gccagccggt ggcgatgagc gacttcatca ccggttttgg gcaggggcgg ggcgcctggg
     3901 cgcgccctgt ggggatcgtc accggcccgg atggcagcct ctatctgagc gacgaccgcg
     3961 ccgggagtat ctggcgcgtc cgctacaccg gcagcagcgg gtcgtagagg cccggactct
     4021 tccctgcctg ccggcttctc gctagaccaa ggcaagagga gctcccgcgt ggcattcagc
     4081 ggtacgccat cccggtccat cgtctcctga tcctgtgtct cgcggcctgg gcgcggcctg
     4141 gcctccgctt atgcctctca cgcgcgctcc tgctcttctt cctctcgcct ggaaggggga
     4201 accccggcgg atatggcgaa ccagcgtgaa taatcgcggt agcgggcagg catgagcgag
     4261 ccctgctccg gaattcgaga ggatgagggc atggagagac ggatccttct ggtttgggtg
     4321 gccgtgctcg cgttgctagt gcccgcggag cgggcaaacg cggccgagct actgtttggg
     4381 tgcgagacag atgccgaggt gtcggcctgg atgctccggt cgggcgggca ggaccggctg
     4441 gcgcgagacc cgggctacgc cacgcaaggc gagttctcca tgcgcttcgc cacccccgcc
     4501 tggaaggatg ggatggcgca gtggcctgcg ttcgaggtcg agccgcccgt ctcggactgg
     4561 cgcggctatg accggctcat gatcgatatc acccatccag gcgatggcga gcacacgctt
     4621 tcgctttttg tctccgacag gaaggtgccg tttcgcgagg ggctgaggta tcggttcgac
     4681 ctcccgaagc ggggctaccg ccggttcatc gtgccactca acttcccaga gaaggtaaac
     4741 cgcggtgata tcggcatcct ccacttcttc acgcagcgcc cgcctagcga gatggtgctc
     4801 tatatcgaca acatcacttt gctgaggccg ggcgagcagc ctcccgagcc acgcgtcggg
     4861 ttcgcggcgc aggtggcgaa gcttttcctg gacagcctcg gtgggctcga cgccgctctg
     4921 gagcaaagcc gcgcctcgct cctgcgcatt gctgataccc cggcgctgcg ccagcgcgcg
     4981 cgcgaggaga tggatgccct ggcggcgtgc gcccgtcggg cgagggagga agccagctct
     5041 cccactctca ccctcgcgcg cctcgagaca atccggcacg agctggaggg gctgccggga
     5101 gaggcccggc gcgtggagtc catcttccgc ttccggaagg cgtttgaggg gttgcgcctg
     5161 ggcgacacgc ccctactcgt tgggtttgcc acctcgatgg agaagctgct cccgcgccgg
     5221 atgccgttcg agctctcggt tgccaggcgc gttgagctcg ccttggcgcg caacgagcga
     5281 gagagcctcc aggtagcggt gatgccccgt gatggggcac tgaagggcgt tcgcgtgagc
     5341 gtgaccgacc tgaagtcgcg ctcgggagcc gtgctgcctg ccgcccggat caattgcgac
     5401 gtggtgggct atgtggagac gaaggcacgc ccgccctacg aagtctcgca cgtgggctgg
     5461 tggcccgatc cgatcctcga ctttctcggc cccgtcgatg tggcttcggg cgacctccag
     5521 agcttctggg ttcgcgtgca cgccacgaaa gaccaagcgc ccggcctcta tgagggcacg
     5581 ctccaggtgg cagcggaagg cgtggagcca ctctctttcc cgctggcggt gcgcgtctac
     5641 aacttcaccc tcccagtcca caccccgctc cccacggcga tcaccttcaa cgagctgaaa
     5701 tcgcagatgg gcggcgcaga gcgctgggcg gagatgaagc accgatgggc cgacttcctc
     5761 gccgactact acatcgacta cgacaacctc taccggcagg gcccgcccga ttgggagatc
     5821 gccaagcggc tccatgacga gggccggctc gtcgcgttca atttggggaa cgtcttcaac
     5881 gcgggcacca aggaggatgg gtttgatgaa gccatcgccg cgacgatagc ccggctgcgc
     5941 ccggcctacg agaaggcgaa ggagctgggc gtcctggatc acgcctacat ctacggcttc
     6001 gacgagcgcc ccaaggagca attcccgctt ctggagcgct ccgcgcgggc gctgcgccag
     6061 gccttccccg aggtcctcct gatgacgacc agctacgacc atagctacgg gctggattcg
     6121 gaagtgaaga cgattgacgc ctggtgcccg ctcacgccca ggtttgaccc ggagcgggtc
     6181 aagcgggcgc gcgccgccgg gaagaaggtg tggtggtaca tctgttgcaa tccgcaccgt
     6241 ccctacgcca actggttcgt cgagtcggac gccatcgagg cgcgcctcct catgggtgcc
     6301 atgacggcga agtaccgccc agacggcttc ctctactacg cgctcgccaa gtggaacaac
     6361 aacaagccga tcgagaccgg accgttcacc gagtggaacc ccgtgagcta tgtcacctac
     6421 cacggagacg gctccatcct ctgcagcggc ccaggcggga agccagtccc cacgatccgc
     6481 ctggagaact accgcgacgg catggaggac ttcgcctatg cctgcatcct ggaggagtgc
     6541 atccggcggt atgaggcgaa gggcgagcgg cttaccgccc gcgagcggga atggcttgcc
     6601 aaggcacgcg aggcgttgcc agtgcccgag acgctggtga atacagtggc cgagtactcg
     6661 cgcgacccga aggcggtcta cgcctggcgc aaccgcgtcg gcgagatgat cgaccggtcc
     6721 ggcgtgcctg acccgaaccc ctggggcaag gcgttcgggg tgcgcggctt tggagggtag
     6781 gcgatggcaa gcgagcgacc caacctcctc ttgatctgcg ccgaccagtg gcgcggcgat
     6841 agcctgggag tggccggcca cccggtggtt gagactccgc acctggacgc gctcgcgcgc
     6901 tccgggactc tcttcacaca ggcctactcg gcgtgcccct cgtgcattgc tgctcgggcg
     6961 gcgctcttca ccgggctttc gccccgtcgc cacggcttcg tcggctaccg cgatggcgtc
     7021 cactggcgtt acccggtgac actcgcgggc acgctggctg ccgccggata tcatacgcag
     7081 tgtatcggga
//
LOCUS       DAOSCV010000083         7083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6805_length_7083_cov_3.737621, whole genome shotgun sequence.
ACCESSION   DAOSCV010000083 DAOSCV010000000
VERSION     DAOSCV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6805_length_7083_cov_3.737621"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLY56_03320"
     CDS             <1..393
                     /locus_tag="PLY56_03320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ20540.1"
                     /translation="GQEAIAKARELGTPTDAEVQSMEGGLQYIDVKVGSGDSPQPGQK
                     VVVHYTGWLVDGTQFDSSRMRNEPFEFTLGQGQVIEGWDVGVATMKPGGVRKLIIPYT
                     MAYGEQGHPPVIPPQATLIFEVELLKVQ"
     gene            complement(454..1137)
                     /locus_tag="PLY56_03325"
     CDS             complement(454..1137)
                     /locus_tag="PLY56_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ20541.1"
                     /translation="MLRAFIFDFDGVIVNSEPVHFSAFQRVLAEEGIQMTWEQYQASY
                     LAMNDRDCFAVALARAGREAGDEQLHALVERKSEYYEVIRDQVPPVPGVQRFIRRAAE
                     RAPVALGSGGLRRDIEPILTRLGLRDSFSAVVTAEDYQLGKPDPECFRTALAQLNDAR
                     GASIAPEECLVIEDSLHGITAARAAGMRCLAVATYYSPDRLVGADRVVRTLEEVDPGD
                     LAPLFAEVR"
     gene            1373..1990
                     /locus_tag="PLY56_03330"
     CDS             1373..1990
                     /locus_tag="PLY56_03330"
                     /inference="COORDINATES: protein motif:HMM:NF037417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20542.1"
                     /translation="MIRNLFRWSKDEIAMEIRSLYLDGEELNYTSVEKNHLALLRAAC
                     RYFGSWKDAVEFAGLDYSKIRKYKTWTKAKIIERIQELHRQEVDLSWRNISTKVDPAL
                     AAAAVRANRFGSWRAALEAAGLNYDEIRRYREWDESLVIDEVRELAEAGEPLNSRDVQ
                     EHTPPLFHAARRRFENWDSTLEAAGLDADRIRKRPRSRETSASCR"
     gene            2207..3175
                     /locus_tag="PLY56_03335"
     CDS             2207..3175
                     /locus_tag="PLY56_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20543.1"
                     /translation="MRTNPFSLFLVVTALGMMRLSAAAAATSPAPPPIRVVVRAFDNL
                     NEEAPLKKLDLRNHLARLVEEELRALPYIQILPRPKPGFFSRDAEFIVRGDYLTLANG
                     GARLSVRILGRDSSTVAAWSEEAASWRTLASPSHASDADEASGKQQSPVRALTARIAQ
                     ELLRQYTLRQLDALRGSPHAVVASIRLEPRKEAYLHGEQVSIVITPEEACYVTALHVR
                     AGNDVRLICPASDQEDGAARAKREYILGGRALPVQSLQPSGAEESRVLIFLSREPLAL
                     SPELRNARTSEEQLRLLQALVEALNALPADRWSDREMALRFLAPSP"
     gene            3305..4396
                     /locus_tag="PLY56_03340"
     CDS             3305..4396
                     /locus_tag="PLY56_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOJ20544.1"
                     /translation="MERAAERRCRPSHGRFLPIHSRIPFRGRVGIPLLCALLFLAWGG
                     TARADQAEPAQPPAAAQEAPSANGQTPPAATKEESPAEEGQKPADGEPAPAVSPPEPK
                     SERQLGPPLAITGLGALEILARDYEESVRYYIEFLGFREEDASGGVGERRCMLVSGPV
                     RITVREQPGKVIRPKRPPWLGIYPTLLSEVTAEAAGLKKAKGVLVDDLVPQGPAGIAG
                     ILKGDIILAIEGKEVASPEELTATIRSFPAGKKALVTLWREGKKRDLAVELPTSPFPE
                     QLGPFAIRLQVRDLDAYYREALRRNLRLYAEPREIPGGTRILELVDPNDLLIVLEESR
                     SEGTAPSPSAAPATPERDAQQQAPGATDA"
     gene            complement(4419..5348)
                     /gene="pfkB"
                     /locus_tag="PLY56_03345"
     CDS             complement(4419..5348)
                     /gene="pfkB"
                     /locus_tag="PLY56_03345"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOJ20545.1"
                     /translation="MILTLTLNPSVDHSVSVARWRKGRTNRPTAFWRDPGGKGINVSR
                     VIHRLGGDTTAYALVGGAPGQLVREALEREGVALNLVEVSGDTRINTIITDLSDGTQT
                     RLNLPGPQVSADELETLYQRLDAAPDPTFFVVGGSLPEGVLPEVYETLIAAFKARGVR
                     TILDADGDPLRCGIRALPYLIKPNEFEIAELVGERLRNLEEFIAAAEELVGKAAEVVV
                     VSLGEQGAVAAGEGHTWRVRSPHVEPVSEVGAGDSMVGAFALALARGESLEDALRWGT
                     AAGAAAVMTPGTELSHPKEIHRLLPQVQVERLR"
     gene            complement(5372..>7083)
                     /locus_tag="PLY56_03350"
     CDS             complement(5372..>7083)
                     /locus_tag="PLY56_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ20546.1"
                     /translation="RQEGERLEPAEDSGLMLLLLAGALCNDACLREAEGRWSIAGDPT
                     EGALLVAAAKAGLTRAELSERYPRLDEIPFDPDTRYMATLHAGPEDERWLFVKGAPER
                     VLEMSACIAWSDEGEGEACPSGELQAPGDERLPARSLSPDARTRVQEANHALAAEALR
                     VLGFACRRLPPEVEKITPELLDEGLVFLGLAGMIDPPRPEARRSIETARRAGIQVMMI
                     TGDHRITARAIAAQLDLLHGDRDVIDGKEIEAMTDEELEARVERIAACARAEPGHKLR
                     IVKALQARGHIVAMTGDGVNDAPALKRADIGVAMGITGTDVAKEASEMILTDDNFASI
                     VGAVEEGRSIFANIRRVVVYLLGTNSGEILTLMAAVIAGYPLPLLPLQLLWINLVTDG
                     APTVALSQEPPHGRVLDEPPRPTNAPIISRALVFRVAFVALFMAAGTIGTFIWAWRNG
                     DLALARTVAFATMSVFQLFNIYNTRSLTDSVFRIGLWSNRWVTWGVLFSAALLVAAIH
                     IEFLQTALRTVPLTLGQWGIVVLVSSTVLIAEEIRKRVAPRLFGLEISNAHETARAPI
                     RVR"
BASE COUNT         1255 a         2386 c         2311 g         1131 t
ORIGIN      
        1 ggacaagagg cgatagcgaa agcacgcgag ttgggcacgc ccacggacgc agaggtgcag
       61 agcatggagg gcggcctgca gtacatcgac gtgaaggttg gatcgggcga ctcgccacag
      121 ccgggccaga aagtggtcgt acactacacc ggatggctgg tggatggcac ccagtttgat
      181 agctcgcgca tgcgcaacga gcctttcgag ttcaccctcg gccagggcca ggtcatcgaa
      241 ggctgggatg tgggcgtagc gacaatgaag ccgggcgggg tgcgcaagct gatcatcccc
      301 tacacgatgg cctacggcga gcagggccac cccccggtga ttccgccgca agctaccctc
      361 atcttcgagg tcgagctgct caaggttcag taaaccgaat ggtcgggcgc ggcgcaccca
      421 ggtgtgccgc gcccggccgg cacggtgatc ctcctaccgg acttccgcaa agagcggcgc
      481 caaatcgccc ggatccacct cttccagcgt ccgcaccacc cgatccgccc ccacaagccg
      541 gtcaggcgag taatacgtag ctactgccag acagcgcatg ccagccgcac gcgcggccgt
      601 aatcccatgc agcgagtcct caatcaccag gcactcctcc ggggcgatgc tggctccccg
      661 cgcgtcgttt agctgcgcga gagccgtccg gaagcactcg gggtctggct tgcccagctg
      721 gtaatcctcg gcagtgacga cggcggagaa ggaatcgcgc agcccgagac gcgtgaggat
      781 gggctcgata tcgcggcgca aaccgcccga accaagcgcc acgggagccc gctccgcggc
      841 gcgacggatg aagcgctgca ccccgggcac gggcggtacc tggtcacgaa tcacttcata
      901 gtattcggac ttgcgttcca cgagcgcgtg cagctgctcg tctccagctt cccggcccgc
      961 gcgcgccagc gcgacggcga agcaatcgcg gtcgttcatc gcgaggtacg aggcctggta
     1021 ttgctcccag gtcatctgaa ttccttcctc ggccagcacc cgctggaacg cgctgaagtg
     1081 aaccggctcg ctgttgacga tcacgccgtc aaaatcgaag atgaaagctc tgagcacatg
     1141 cgcctccctc gagggtcaaa cagtcgcggc gtaccagcga gttcgccgct ttgggagagc
     1201 gctcctgccg gcgtgccggg ctccacggac gccaagggtg ggaactccgg ggcccctcgc
     1261 ggtgtcttat gaagatggga cggcgcccct gcaggcggtt gttcgcgttt acaccggggc
     1321 accgccatgt tataatcata gaagattcca acgttgcggt gagggaggta cgatgataag
     1381 gaatctgttt cgttggagta aggacgagat tgcgatggag attcgcagtc tctatctcga
     1441 tggggaggaa ctaaactaca cttccgttga gaagaaccat ctggcgctct tgcgcgcggc
     1501 gtgccgctac ttcggctctt ggaaagacgc cgtagaattt gccgggctcg attacagcaa
     1561 gatccgcaaa tataagacgt ggaccaaggc caagattatt gagcgcatcc aggaactgca
     1621 tcgtcaggaa gtcgatctga gctggcgcaa catcagcacc aaggtggatc cggcactcgc
     1681 ggccgccgcc gtgcgcgcca accgcttcgg gtcgtggcga gccgccctgg aggctgccgg
     1741 cctgaactat gatgagatcc ggcgataccg cgaatgggac gagtcgttgg tgatcgacga
     1801 agtgcgcgag ctggcagagg cgggcgagcc gctcaactcg cgcgatgttc aggagcacac
     1861 gcccccgctc ttccacgcgg cgcggcggcg cttcgagaac tgggacagca ccctggaggc
     1921 cgctggcctg gacgccgacc gcattcggaa gcggccccgc agccgcgaga cgagcgcatc
     1981 ctgccgctga tattgatggc atgatttctg tttttttggg cgggaacccc tagttcccgc
     2041 cccttttttg agttcgatcc acctgctgca cgcgcgcggc ttgagcaacc atgcccgtga
     2101 gccaggtgtt ccgatgggat cccgcggatc gaccgcctct cgcgcgccca ccagcattcc
     2161 gaaagccagg ggcgccaaac ctgatacgaa aatacgaggt accaacttga gaaccaaccc
     2221 cttctctctc ttcctggtag tcaccgcatt gggcatgatg cgactctcgg cagcagcagc
     2281 cgccacgtcc ccggccccgc cgcccatccg cgtcgttgtc cgcgccttcg acaacctgaa
     2341 tgaggaagca cccctgaaaa agctggatct gcgcaaccac ctggctcgcc tggtggagga
     2401 ggagctgcgc gcgctcccct acatccagat cctcccgcgc ccgaagcctg ggttcttctc
     2461 gcgggatgcc gagttcatcg tgcgtggcga ttacctcacc ctggcgaatg gaggcgctcg
     2521 gcttagcgtg cgcatccttg gtcgtgatag cagcaccgtg gccgcctggt ccgaggaggc
     2581 cgcaagttgg cgcactctcg cgagcccgag tcatgcaagc gatgcagacg aggcgtctgg
     2641 caagcagcaa tcacccgtga gggcgctgac ggcgaggatc gcccaggagc tgctgcggca
     2701 gtacacgctg cgccagctcg atgcgcttcg cggatcccca cacgccgtcg tcgcctccat
     2761 tcgcctggag ccaaggaaag aggcctacct gcatggcgag caggtctcca tcgtcatcac
     2821 ccccgaggag gcctgctacg taacggcgct tcatgtgcgc gcgggcaatg acgtgcgcct
     2881 gatctgcccg gcgtctgacc aggaggacgg cgccgccaga gcgaagagag agtacatcct
     2941 gggcggacgg gcgttgccgg tccaatcgct ccagccgtcg ggcgccgagg agagccgggt
     3001 gctgatcttt ctatcccgtg aaccgctggc actctcgccc gagctacgca acgcgcgcac
     3061 gtccgaagag cagctccgcc tgctccaggc cctcgtcgag gcgctgaacg cccttccggc
     3121 ggatcggtgg tcggaccgcg agatggcgct gcgcttcctg gcgccctccc cataaccgga
     3181 cgagacgcca cgccgggcgc ggcagggcgg agagagaacc gccgcgggca acgtgaagga
     3241 gcgcaaggtg aatcggagga ctgggttgtc tcctctgcga atgagtcgag ggaacaggca
     3301 gggaatggaa cgcgccgcgg aacggcgttg ccgcccatcc cacgggaggt ttctgcccat
     3361 tcactcgcgc attccgttcc gggggcgcgt gggaatcccg ctgctgtgcg cgctgctgtt
     3421 ccttgcctgg ggcggcacgg cgcgcgccga tcaggcagag ccggctcagc cgcccgcggc
     3481 ggctcaggag gccccatctg ccaatggcca gacgccgccg gccgccacaa aggaagagtc
     3541 gcctgcggaa gaagggcaaa agcccgcgga tggagagccc gcgccggccg tatcgccccc
     3601 cgagcccaag tcggagcgcc agctgggccc gcctctggcc atcactggtc tcggcgcgct
     3661 tgagatcctg gcgcgcgact acgaagagag cgtgcgctac tatatcgagt tcctcggctt
     3721 ccgcgaggag gatgcgtctg gaggcgtggg ggaacgccgg tgcatgcttg tttcgggtcc
     3781 ggtgcgcatc accgtgagag agcagccggg caaggtgatc cggccaaaac gcccgccgtg
     3841 gctggggatc tacccaaccc tcctgagcga ggtaaccgcg gaggccgccg gcctgaagaa
     3901 ggcgaagggc gttctggtgg atgacctggt gccccaaggc cccgccggga tcgcgggcat
     3961 cctgaagggc gatatcatcc tggccatcga gggcaaagag gtcgcctcgc ccgaggagct
     4021 gacggccacc attcgcagct tccccgccgg caagaaggca ttggtcacgt tgtggcgaga
     4081 agggaagaag cgcgatctgg cggtggagct acccacctcg cccttccccg agcaactggg
     4141 ccccttcgcc attcgcctgc aggtgcgtga tctggacgcc tattaccgcg aggcgctgcg
     4201 ccgcaacctc cgcctgtacg ccgaaccgcg cgagatcccc ggtggcaccc ggatcctgga
     4261 gctggtggac cccaatgacc tcctgatcgt cctggaggaa tcccggtccg agggcacggc
     4321 cccctctccg agcgcagctc cggcaacgcc tgagagagac gcgcagcagc aagcgcccgg
     4381 cgcgacggac gcctgattca ccgcacgctc gcctgccctc agcggagcct ctccacctgc
     4441 acctggggca gcaggcggtg gatctccttg gggtggctca actcggtacc cggggtcatg
     4501 actgccgcag cacctgcggc agtgccccac cgcagcgcgt cttccagcga ttcgccacgc
     4561 gcaagcgcca gggcaaacgc gcccaccata gagtcgccgg cgcccacctc gctcaccggt
     4621 tcgacgtggg gcgagcgtac gcgccaggtg tggccctcac cggccgcaac cgctccttgc
     4681 tctccgaggg agaccacgac cacctccgcc gcctttccaa ccagctcttc ggcggcggcg
     4741 atgaactcct ccaggttgcg caggcgctcg ccgaccaact ccgcgatctc gaactcgttc
     4801 ggtttgatca ggtagggcag cgcgcgaatg ccgcaccgga gcgggtcacc atcagcatcg
     4861 aggatggtac gcacgcccct tgccttgaac gcggcgatca gagtttcgta cacctcgggg
     4921 agcaccccct caggaaggct accgcccacg acgaagaagg tgggatcagg cgccgcgtcc
     4981 aggcgctggt aaagcgtctc cagctcatcg gcgctcacct gcggcccagg gaggttgagc
     5041 cgggtctgcg tgccatccga aagatcggtg atgatcgtat tgatgcgcgt gtccccactc
     5101 acctccacca ggttgagggc gactccctcg cgctccagcg cctcgcgcac cagctggcct
     5161 ggcgcgcctc cgaccagggc gtaggccgtc gtgtcgcccc cgagacggtg gatgacgcgt
     5221 gagacgttga tccccttgcc gcccggatcg cgccagaagg cggttggccg gttggtgcgg
     5281 cccttcctcc aacgggccac gctcacgctg tggtcaacgg aggggttgag cgtcaacgtc
     5341 aggatcatcg tgcctctccc ctcgtggcgc ttcaccgcac ccggatggga gccctggccg
     5401 tctcgtgcgc gttcgagatc tccagcccga agagacgagg cgccacccgc ttccgaatct
     5461 cctcggcaat cagtacggtg gaggagacca gcaccacgat cccccactgg cccagggtga
     5521 gcggcactgt ccggagcgcg gtctggagga attcgatatg aatcgcggcg acgagcagcg
     5581 ccgccgagaa gaggactccc caggtgaccc accggttgct ccagaggccg atgcggaaga
     5641 ccgaatcggt gagcgagcgc gtgttgtaga tgttgaacag ctggaagacg gacatcgtcg
     5701 cgaacgccac ggtgcgcgcc agcgccaggt caccgttgcg ccacgcccaa atgaaggtgc
     5761 cgatggtacc cgccgccatg aagagagcga cgaaggcgac gcgaaagacg agcgcgcggg
     5821 agataatggg cgcgttcgtc gggcgcggtg gctcatcgag gacgcgacca tgcggcggct
     5881 cctggctcag cgcgacggtt ggcgcgccat cggtgaccag gttgatccag aggagctgca
     5941 gaggaagcag cggcaatggg tagccggcaa tgaccgccgc catcagcgtc aggatctcac
     6001 cgctgttggt gccgagaaga tagacgacga cgcggcggat gttggcgaag atcgatcgcc
     6061 cttcctccac cgctccgacg atggaggcga agttgtcatc ggtgaggatc atctcgctcg
     6121 cctctttggc cacgtcggtg ccggtgatcc ccatcgccac gccaatatcg gcgcgtttga
     6181 gcgcgggcgc gtcgttcacc ccatcgcctg tcatcgcgac gatatgcccg cgcgcctgca
     6241 acgcctttac gatgcgcagc ttatgcccag gctcggcgcg cgcgcacgcc gcgattcgct
     6301 cgacacgcgc ctccagctcc tcatctgtca tcgcctcgat ctctttgccg tcgatcacgt
     6361 cgcggtcgcc atgcaagaga tccagctgcg cagcaatcgc gcgcgcggtg atccggtgat
     6421 cgcccgtgat catcatcacc tggatgcccg cccggcgcgc cgtctcgatg gagcgtctcg
     6481 cctcgggccg cggcgggtca atcattccgg cgagaccgag gaacaccagg ccctcatcca
     6541 gcagctcagg cgtgatcttc tctacttccg gggggaggcg gcggcaggcg aagccgagca
     6601 cgcggagcgc ctctgcagcg agggcgtggt tggcctcctg aacgcgcgtg cgtgcatcgg
     6661 gcgagagact ccgcgccggc agcctctcgt ccccaggcgc ctgaagctcg ccgctcgggc
     6721 acgcttctcc ctcgccctca tcactccagg cgatgcacgc gctcatttca agcacgcgct
     6781 cgggtgcgcc cttcacgaag agccagcgct cgtcttccgg cccggcgtga agcgtggcca
     6841 tgtagcgcgt gtccggatcg aacgggatct catccaggcg cgggtagcgc tccgacagct
     6901 ccgcccgggt gagaccggcc ttggccgcgg cgacgaggag ggcgccctcc gtcgggtcgc
     6961 cggcgatgga ccaccggccc tccgcctcgc gcaggcaggc gtcgttgcaa agcgcccccg
     7021 ccaggagaag gagcatcaag ccggagtcct cggcgggctc cagcctctcg ccttcctggc
     7081 gga
//
LOCUS       DAOSCV010000084         7083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6806_length_7083_cov_2.988048, whole genome shotgun sequence.
ACCESSION   DAOSCV010000084 DAOSCV010000000
VERSION     DAOSCV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_7083_cov_2.988048"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1853)
                     /locus_tag="PLY56_03355"
     CDS             complement(249..1853)
                     /locus_tag="PLY56_03355"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOJ20547.1"
                     /translation="MLYWSAGAEGPRPERPGAKESFSRHQRAPLSPSRARRPVRRSNC
                     MALRIAVFGSGAVLMALEILAFRIIGKTFGTALRETTAVIAIFLISMSLGYWLGGKAG
                     DRWPCRRTLVLPMAGAGLYILFIPLLDETISERVFDSALPLGLHALTACALFFAVPSL
                     LMATVSPVAIRLLAQAVEQTGKVAGSVSALSTVGSILGTILMGFYLIDAIGSAKLLTV
                     ALGGTMLALSALAALGMRLKPAAAGLALLLLAGSGASANIIYERDSSYHHIVVREEDG
                     FRILYFDNAPQSQMSVADPTSGAFEYCDYFSSAFLFKPDIKDVLMLGLGGGSIPKRFL
                     KDNPNVRMDVVDVDAQVVAVARRFFAVQPGPRLNLIVADGRAYLKRTRKKYDYILIDA
                     YTRNRYGSTIPVHLTTREFFEQVSKCLRPGGIVAYNVIAQVGHANDGIITALLRTMDA
                     HFTTPYLFQAESSWNTVIMAFKGTPQRLTRDALLRRAQEAVRAGRIKLPGFEKRAGNI
                     VPVPDLKNAILLTDDNAPVDRLLRGR"
     gene            2029..2673
                     /locus_tag="PLY56_03360"
     CDS             2029..2673
                     /locus_tag="PLY56_03360"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ20548.1"
                     /translation="MRLVLLGAPGAGKGTLAKQLSKALGVVHISTGDIFREEVAAGTE
                     LGKKAKSYMDRGALVPDEVVIGMVKQRLSRPDVNAGFILDGFPRTVPQAEALDKVLEE
                     SNQPLDAVLDIVVPEETVVRRLSGRRVCRQCGAIYHIDNMPTKQEGVCDKCGGEVYQR
                     DDDQPEAIRQRLKVYAEATAPLTDYYRTKGLLRPVDGTGTPEEVLGTALALLKG"
     gene            complement(2761..3750)
                     /locus_tag="PLY56_03365"
     CDS             complement(2761..3750)
                     /locus_tag="PLY56_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOJ20549.1"
                     /translation="MDVYPLRFSDLFMERVWGGRGLERVLGKTLPPEKRIGESWEVSD
                     QPGAMSAVRNGAYAGLTLGELRERFPEELMGPRGLAMGRGRFPLLIKFLDCEDRLSVQ
                     VHPDDAYAAAHEGGSLGKTEMWYVVAAEPGAVLWCGLKPGIDRPALESAIAEGRVPET
                     LAEVPVSPGDCFFIPAGTVHALGGGLIICEIQQNSDVTYRFYDWGRVGLDGRPRELHV
                     QKSLDVIRYGADRNPRCLVQEMDVPGATVARLVTCPEFAAEKLVITGLLESNTGETSF
                     HALSAVMGEGHVRAGEGAPEPLRRGETLLIPSAAGRYEIESAGELVVLRAFVP"
     gene            complement(3998..6901)
                     /locus_tag="PLY56_03370"
     CDS             complement(3998..6901)
                     /locus_tag="PLY56_03370"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOJ20550.1"
                     /translation="MSLQDGVLVAEGVDLRGEIQRHADGRLALEARLGETNIDALRAL
                     AMKVAALAPEPEKGARAAGFGTLLRDAVAGLPDPLTGTVAAEIALEGSRGALDGQARL
                     QLEDLLMGEARFQSARATVRWDRGHMAADDLALHSEGVDLRGSFQRGTDGHLALQLHL
                     GETEMAAARDLARKVASALQGTQIGRFLPESLPGVLADLPEPLEGSLSGSFSLAGKPG
                     ALRGSINARAQDLRLGQSGITAVSAEGTWSPEEWNFPAIVVAGEKCALSGSIAREPGG
                     RLAGEIVTRDGCLEGLRSMASALSLLRRFPVAQELMARIDSLPTPLAGNIDGSIRFSG
                     TLLDPEMRVALTGSNLVAGTYKLAGVTADADVKGNLVTVRDLRVRTNGGELSARGHVD
                     LDGPMALTVVGNDFSLEALGPLLPNGRKLTGHVDLSANVGGTTDIPEADARIVVRNAT
                     IAGMRFSRIEANDVRLTERRVEFGRVDLEQGPLAGTLSGSVPFRWDPLEIPTDEPVQL
                     SVSLSQFDLSTAMLANDQSTGIKVSSTPEMRPLLDAIESASGKTMAEIVVTGTLREPN
                     MDGRIELHEGQVKLAALDNHLRDVEASLRLEGPWIIVEQLSAKSDGGGVLSATGRAGI
                     RAQGDQMLDVRLHADRFQLGVRDLAGVHGLGVKGVTSGDLALVGRLAAPVLTGKLQVS
                     KAQMNVPSEITPRLARPPLTLLDPALNIDVEVDQDSWVRRGNAISARVSAGIGIRGKL
                     SNPAVSGRATVERGDLRYAGISFRLQPSGTVSFSYDTPGALVARLDLTADTRVRAAAP
                     ATGERRRYIVTMHVTGTIPNVNVAVTSDPPDLPQHRLMAILARTDQFEALARGDNMMD
                     EVLRDQVVDIVAGAVLPQVFEPIEEGLAEALGLDEVNFDYGFDRPLRVDARKEVLPRL
                     FVSYNRDMGQGQFVDSGEQWSVTYRLYQRLYVGWRRDTTGNKDRLVLEGSFKF"
BASE COUNT         1251 a         2431 c         2269 g         1132 t
ORIGIN      
        1 aggccgttac actccgccgg tgtttttcac cagcaggcgg gtggaaagcg ggggctcgga
       61 gagcaaacgg gttggccctt tattcgacgc agagcagtgt actcttgcca ggagagacgg
      121 tttccatggc cggggcttcg agaggcgtga gagcgcccgc ggcaccgggc gccggggttg
      181 cactacggtt cagggagagt ggaatggttc ccgggaggtg ctccgagagt gggagcgccc
      241 tgcttcgctc agcgtccgcg gaggagacgg tccaccggtg cattatcatc cgtgaggagg
      301 atcgcgttct ttagatcggg cacggggacg atattgccgg cgcgcttctc gaaacctgga
      361 agcttgatcc ggccggcgcg gaccgcctcc tgggcccggc gaagcagagc gtcgcgggtc
      421 agccgctgcg gcgtgccctt gaatgccatg atcacggtgt tccagctact ctccgcctgg
      481 aaaagatagg gcgtggtgaa gtgggcgtcc atcgtcctca gcagcgcggt gatgatccca
      541 tcgttggcgt ggcccacctg ggcaatcacg ttgtaggcga cgatcccgcc tgggcgcaga
      601 catttgctca cctgctcgaa aaactcgcgc gtggtgagat ggaccgggat ggtggagccg
      661 tagcggttgc gagtgtaggc atcgatcagg atgtagtcat acttcttccg ggtgcgcttc
      721 aggtaggcgc gaccatcggc cacgatcagg ttgagccggg gtccgggttg caccgcgaag
      781 aatcgcctgg ccacggcgac gacctgggca tcgacatcga ccacatccat gcgcacgttg
      841 gggttatcct tgaggaagcg cttggggatg gagccgcccc cgaggccgag catcaggaca
      901 tccttgatat cgggcttgaa gaggaaggcg gaactgaaat agtcgcagta ctcaaaagcg
      961 ccggaggtcg ggtcggccac actcatttgg ctctgggggg cgttgtcgaa gtagaggatg
     1021 cggaagccgt cttcctcgcg cacgacgatg tggtggtagc tgctgtctcg ctcgtagatg
     1081 atgttggcgg aagccccgct gccagccagc agcaggagag ccaggcctgc cgcagcgggc
     1141 ttcaggcgca ttccaagcgc ggcgagagca gatagtgcca gcatggtgcc tcccaatgcc
     1201 accgtcagga gcttcgcgct cccgatggcg tcgatcaagt agaagcccat caggatcgtg
     1261 ccgagaatgc tgcctaccgt ggagagcgcg gagacgctcc ccgccacctt gcccgtctgc
     1321 tccactgctt gcgcgaggag acggatggca accggcgaca cggtggccat gagcagcgag
     1381 ggcaccgcga agaagagggc gcacgccgtg agagcatgta acccgagcgg gagcgcggag
     1441 tcgaagaccc gctccgagat cgtctcgtcc agcagcggga tgaagaggat gtagagcccg
     1501 gcgccggcca tcgggagcac gagcgtgcgc cggcacggcc aacggtcgcc cgctttgccg
     1561 ccgagccagt agccaaggct catcgagatc aggaagatgg cgatcacggc cgtcgtctcg
     1621 cgcaaggcgg tgccgaaagt cttgccgatg atgcgaaagg cgagaatctc cagggccatg
     1681 agcacggccc cgctgccaaa caccgctatc ctcaacgcca tgcagttgct ccttctaacg
     1741 ggcctgcgcg cgcgagacgg ggacaagggc gctcgctggt gccgcgagaa gctctccttg
     1801 gccccaggcc tttccggccg cggcccctcc gcgccggcac tccagtatag cacgcgccgc
     1861 aaagaggcgt caacggacgg gcacggctcg cgcaaggagg atggcgggcg gcaggcgaac
     1921 tcaaaggagt gtctggaggc gcgagaagca cccttggtct ggcagcgccc agacagtccg
     1981 ccccggtaca gaccaacact ccgcgccggg gcgatgggag gagatagaat gcgattggtt
     2041 cttctgggcg ccccgggcgc cggcaaaggc acgcttgcca agcaactctc caaagctctt
     2101 ggcgtcgttc atatctcgac gggcgacatc ttccgcgagg aggttgcggc cggcacggag
     2161 ctgggcaaga aggcaaaaag ctacatggat cgcggcgccc tggtgcccga cgaggtcgtc
     2221 atcggcatgg tgaagcagcg cctctcccgc cccgacgtga acgcgggctt catcctcgat
     2281 ggcttccctc gcaccgtccc ccaggccgag gccctcgata aggttcttga ggagagcaac
     2341 cagcccctcg atgccgtcct cgacatcgtc gtgcccgagg agaccgttgt ccgccgcctc
     2401 tccggccggc gcgtctgccg ccagtgcggg gcgatctatc acattgacaa catgcccacg
     2461 aagcaagaag gcgtctgcga taagtgcggg ggcgaggtct accagcgcga tgatgaccag
     2521 cccgaggcga tccgccagcg cctgaaggtc tacgcagagg caaccgcgcc gctgaccgac
     2581 tactaccgca ccaaggggct cctgcgcccg gtggatggca ccggaacgcc ggaggaggtg
     2641 ctcggcaccg cgctggcgct gctgaaggga taaagcacca cctgaagcat gaaggcatgg
     2701 aagcgcggcc cgcacccgtc gcggacgcgg cttccatcct tcccccgcat caccccatcg
     2761 ttacgggacg aacgcgcgca acaccaccag ctcgcccgcc gattcgatct cgtagcgacc
     2821 agcggcgctg gggatgagga gcgtctcgcc ccgacgcaac ggctccggcg cgccctcccc
     2881 ggcgcgcacg tggccctcac ccatcaccgc ggaaagcgcg tggaaactcg tctccccggt
     2941 gttagactcc agcaaccccg tgatgaccag cttctcagca gcgaattctg gacaggtcac
     3001 caggcgggct accgtcgcgc cgggcacatc catctcctgc accaggcaac gcgggttgcg
     3061 atccgccccg taccggatca catccagcga cttctgaacg tgcaattcgc gcggacgacc
     3121 atcgaggccg acgcgccccc aatcgtagaa acggtaggtc acatccgagt tctgctggat
     3181 ctcgcagatg atcaggccgc caccgagcgc gtggactgtg cccgcgggga tgaagaagca
     3241 gtcgccgggc gagacgggca cctccgccag cgtttccgga acgcgccctt cggcaatggc
     3301 gctctccagc gccggccggt cgatccccgg cttcagcccg caccagagga ctgctcccgg
     3361 ttccgccgcc accacatacc acatctcggt ctttccgaga gatccaccct cgtgggccgc
     3421 cgcgtaggcg tcatccgggt gcacctggac ggaaagccgg tcctcgcaat cgagaaactt
     3481 gatcaggagc gggaagcggc cgcggcccat cgccaggccg cgcggcccca tcagctcctc
     3541 cggaaaccgc tcgcgcaatt cgcccagggt caaacccgcg tacgcaccgt tgcggaccgc
     3601 gctcatcgcc cccggctgat ccgagacctc ccacgactcg ccaatcctct tctctggagg
     3661 cagcgtcttt cccaacacgc gctccagacc acgtcctccc cacacgcgct ccatgaagag
     3721 atcactgaac cgtagcgggt aaacgtccat atcgctctgg ggtgcagggg gagagaaact
     3781 ccagaatcct cctcgctccc cctggatgcg gggaggagag agccccctgc agctcccacc
     3841 ctcctctcac gtctttttcc caggtatcga tcaggcgcca gggattccca cccgccattc
     3901 ctctcatgcg agaggcgcgg gttcccccac tctccatcat acccacgttt gcccagcagg
     3961 acacgggcgg cttgcccggc gctggggtgg cgctcgccta gaacttgaag gagccttcga
     4021 gcacgaggcg gtccttgttg ccggtcgtgt ctcgccgcca gcccacgtag agccgctggt
     4081 agaggcggta ggtcacgctc cactgctcgc cgctatccac gaattgaccc tggcccatgt
     4141 cgcggttata ggagacgaag agccgcggca gcacctcctt gcgcgcgtcc acacgcagcg
     4201 gtcggtcgaa gccgtagtcg aagttcacct cgtcgagccc tagggcttcc gccaggccct
     4261 cctcaatcgg ctcgaacacc tgcggcaaga cggcgcctgc cacgatatcg accacctgat
     4321 cgcggaggac ttcatccatc atattatcgc cccgggccag cgcctcgaac tggtcggtgc
     4381 gcgccaggat cgccatcagc cggtgttgcg gcaggtccgg cggatccgag gtcacggcga
     4441 cgttgacgtt cgggatcgtt cccgtgacgt gcatcgtgac gatgtatcgc cggcgctctc
     4501 cagtggccgg cgccgccgcc cgaacgcggg tatccgcggt caggtcgaga cgggccacca
     4561 gcgcccccgg agtgtcataa gagaaggaga cggttccgct gggctgtagc cggaaggaaa
     4621 taccggcata gcgcaggtca ccgcgctcca ccgttgcgcg cccgctcacc gccggattgc
     4681 tcagtttgcc ccgaatgccg atgcccgcgc tgacccgcgc cgagatggca ttcccacggc
     4741 gcacccacga gtcctgatcg acctccacgt cgatattcag cgccggatcc aggagcgtca
     4801 gcggcggccg tgccaggcgc ggcgtgatct cggagggcac attcatctgc gccttcgaga
     4861 cctgtagctt ccctgtgagc accggtgctg ctagcctgcc gaccagggcc aggtcgccgc
     4921 tcgtcacgcc cttgaccccc aggccatgca cgccggccag atcgcgtacc cccagctgga
     4981 agcggtcagc atgcaggcgc acatcgagca tctgatcccc ctgggcacgg atccccgccc
     5041 ggccggtggc gctgagaacg cctcctccat ccgactttgc cgagagttgc tccacgatga
     5101 tccacgggcc ctccaggcgc aggctcgcct ccacgtcgcg gaggtggtta tcaagggccg
     5161 ccagcttcac ctggccttca tgcagctcga tccgcccatc catgttcggc tcgcggagcg
     5221 tgcccgtgac gacaatctcg gccatcgtct tccccgacgc ggactcgatg gcatcgagca
     5281 gcgggcgcat ctccggcgtg ctgctcacct tgatcccggt gctctgatcg ttggcgagca
     5341 tcgctgtaga aagatcgaac tgcgagagcg acacgctcag ctggaccggc tcgtcggtcg
     5401 ggatctcgag aggatcccag cggaaaggca ccgagccaga gagcgtgccc gcaagcggcc
     5461 cctgctccag atcgacacgc ccaaactcca cgcggcgctc cgtgaggcgc acgtcgttgg
     5521 cttcaatgcg cgagaagcgc atgccggcaa tggtggcgtt gcgcacgacg atcctcgcgt
     5581 ccgcctcggg gatatccgtc gtgcccccta cgttcgccga cagatcgaca tggccggtga
     5641 gcttgcgtcc attcgggagc agtggtccga gcgcctccaa cgagaagtcg ttcccgacca
     5701 cggtcagcgc cattggtccg tcgagatcga catgaccgcg cgcgctgagc tcgccgccat
     5761 tcgtgcgcac tctcaggtca cgcacggtga cgaggttgcc cttcacatcg gcatcggcgg
     5821 tcacgcccgc gagcttgtag gtgcccgcga ccaggttcga gccggtcagg gccacccgca
     5881 tctcggggtc gagcagcgtt ccagagaaac ggatgctccc atcgatattg cccgcgagcg
     5941 gggtcggaag cgaatcgatc cgcgccatca actcctgcgc cacggggaag cggcgaagga
     6001 gagaaagcgc gcttgccatc gaccgcagcc cctccaggca gccgtctctc gtcacgatct
     6061 cacccgcgag ccggccaccc ggctccctgg cgatcgatcc agagagtgcg cacttttcgc
     6121 cagcgactac aatcgccggg aagttccact cctcgggcga ccaggtgccc tccgcggaga
     6181 cggcggtgat cccggattgg cccaggcgca gatcctgtgc gcgggcgttg atgctcccgc
     6241 gcagcgcgcc aggctttccg gccagcgaga agctgccgct caggctcccc tccaggggct
     6301 ctggcagatc ggcaagcacg ccagggaggg actcggggag aaagcgcccg atttgcgttc
     6361 cttgcagggc agaggccacc ttgcgtgcca gatcgcgcgc cgcggccatc tccgtctctc
     6421 cgaggtgaag ctgcagcgcg aggtgcccat ccgtgcctct ctggaagctg ccgcgcagat
     6481 ccacgccctc ggaatgcagg gcgagatcat ctgcggccat gtggcctcgg tcccagcgca
     6541 ccgtggcgcg cgcgctctgg aaccgcgcct cgcccataag cagatcctca agctggaggc
     6601 gcgcctggcc atcaagcgcg ccgcgggagc cctccagcgc gatctccgcc gctaccgttc
     6661 cggtgagggg atcgggcagg ccagcgaccg cgtcgcggag gagcgttcca aacccggctg
     6721 cacgcgcgcc cttttccggc tccggagcga gcgctgccac cttcatcgcc agcgcgcgca
     6781 acgcatcgat gttcgtctcg cctaggcgtg cctctagcgc caggcggcca tcggcgtgcc
     6841 gctggatctc gccgcgcaga tccacgccct cagcgaccag cacgccgtct tgcagtgaca
     6901 tctcttgagg cgtccaggcg gcggagcccg ccagacgctg tattttcaaa tcacccagct
     6961 cggcctgagc gagaactcct tgcgcgcgcc ccgagagctc cggccatcgt cctgagagcg
     7021 cgaaagtgcc gtcgaagcga cctcgcatgc ctgccgggag cgcggcaacg cgctgctgct
     7081 gct
//
LOCUS       DAOSCV010000085         7063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6833_length_7063_cov_3.435645, whole genome shotgun sequence.
ACCESSION   DAOSCV010000085 DAOSCV010000000
VERSION     DAOSCV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6833_length_7063_cov_3.435645"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1985)
                     /locus_tag="PLY56_03375"
     CDS             complement(33..1985)
                     /locus_tag="PLY56_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ20551.1"
                     /translation="MTEAAVEFDRRAAVVRHLNQHFPAPQFESREEWEARARELRAHI
                     LASAGLSPMPERCPLDPIVTGEIEGDDYVIRRVAIQTLPGFYLGGNLYLPKGKQGPFP
                     GIANPHGHWKTGRIEHQERGSIPARCITFARMGCVAFAYDMIGYNDTRQLPHTFGGDR
                     EALWGISLGGLQLWNSIRALDYLESLPMVDPERLACTGASGGGTQTFLLSAVDDRVKV
                     AAPVNMISLHMQGGCLCENPPNLRVECTNVEIGALMAPRPLLLVAATGDWTRNTLEEE
                     FPAIQAVYRLMGAEEKVHALRFDAPHNYNLQSREAVYAWMGRWLLGINDPDQLKEQPI
                     DLPDPKVLLTFPDGQLPEGAADQEGITRYLVERSEKQLAAHWPRDQETARQFRETYLP
                     LLSHSLGAAAPAPELVTATPAGGSTRAQRLILGRKGAGDRVPARLHRSRRQKGSAPGV
                     LLVTPEGIRAADGPGITSRLVGQLSREDRHVLLIDTFLTGDSKAPETREKEIAEIKYF
                     TTYNRTDIGNRVQDILTALAFLRAQEGVSRVQVVGVGEAGLWAVLACALDGRVDAAAI
                     DLAGFEMTDDHFLEKLNVPFLRRAGDLVTAAALCAPRRLSFWRVHPFFPMGKVFDAYA
                     ASGKPGAVQFTSMALDAEAVQAWLGL"
     gene            complement(2165..3649)
                     /locus_tag="PLY56_03380"
     CDS             complement(2165..3649)
                     /locus_tag="PLY56_03380"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HOJ20552.1"
                     /translation="MSDAGPGLVLSLDIGTSSTRAALYTSQGRPVPGAESSRPQRVHT
                     TADGGAEMHPEEVVQRAVRCAAEAVERAGSQARRIVGVGVCTYWHSILGVGANGRATT
                     PIYLWSDTRSVAEVEALRQRLDAPAVYARTGCPLHTSYVGPRILWIARTQPESFRRSQ
                     RWMSIGEYLFLRLFGEAACSHSMASGTGLFDLGRLRWDPEVLAALPIEEEQLSTPVAH
                     SRASVGLRPEFARVLPNLSDVPWFPPLGDGACSNVGSGAVSRRRVAIMVGTSGAMRVL
                     CPTGGPTPPQGLWAYLLDERRFLLGGALSNGGNFYTWLTRTLRVGQRERLEEALSRME
                     PDAHGLTVLPFLMGERSPGWRGDARAVIAGLSAHTSPHEIIRAGLESVAYRFALIHER
                     LRDRLDAGPDYRVIATGGALLYSPLWMQIIADVLNRPVVASREKQASGRGAALMALEA
                     LGMIRGIQSAPFAFGKVYHPDAARHARYMEALARHTALYRRLLE"
     gene            complement(3646..4938)
                     /locus_tag="PLY56_03385"
     CDS             complement(3646..4938)
                     /locus_tag="PLY56_03385"
                     /inference="COORDINATES: protein motif:HMM:NF036726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HOJ20553.1"
                     /translation="MHLDELLKRLNETQSLEALQPHWEESLGSLGEGLPSFLTPEEYR
                     ENREWCGFGPEVDALLDEAARRIAADRGLCALAWHCHRLLFTHLDYTDQAFLRSWPSL
                     QGVLGELRGVFYLLAVMGMVPTVRRLHASMDVPEDVTRETLSQVSCVAGNYRRMTGGL
                     LGVTVNTLFWMRHYPAGKLFRLGRFEYKIEPFRQGIRVFRSRKTGETLALAPDGVRYN
                     GKGHVDGTAGCMDIARGWTAMLTEDEDSVTGYPISPYGMAVRTRVTLPKAEWECVLKT
                     GDPILEMHIPAGGGMTLDRCGDSMRRAAAFFRRYFPDKPTRAITCVSWIFNTQFEEIE
                     PRMENLLAYQHELYLHPVTSSGRDGLWFVFLRDDYDPATLPRDTSLRRGVADFLAAGN
                     IWRCGGMFFLFDDLERFGTQAYRAQWPVSVLKRQGTTL"
     gene            complement(5166..5726)
                     /locus_tag="PLY56_03390"
     CDS             complement(5166..5726)
                     /locus_tag="PLY56_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ20554.1"
                     /translation="MEFMDVLTARRSVRSYTPTPLDRATIEKLIHAAIQAPSATNTQP
                     WAFGVITGVERLKEYGDRVKAKLLPALDQMPELERYRDMITDPKYNVFHGAPALVVIY
                     GKPASLLPEIDCALAAQNLMLAAAAMNLGTCWIGFAQEFFDPPAVKAELGVPESYKAI
                     APIIVGHPAGTTPPVPRNTPEVVYWM"
     gene            6021..6854
                     /locus_tag="PLY56_03395"
     CDS             6021..6854
                     /locus_tag="PLY56_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20555.1"
                     /translation="MSEFPRTMVGGVSMPRLICGSNWFLGYSHTSRAKDRLIKELFDT
                     PAKMARVVEVFARRGCNAFMSMQSQFVAEALREVEQRVGVPMLWVTTPTYKEPGNPDT
                     WKASVDEARELGATFCFPHQCVTDPRIDRVNRCLSPQLIEHLAYVREAGMVPGLSSHA
                     PEAITCSDACGADVETYITPYNAAGFLCQVETDWIQRIIHSAKKPVMTIKPLAAGRLL
                     PPTGLSFVWSTLRDCDLVTIGTMSTYEAEEVIEISLACLEQRKAEVELQYTRSKSTLV
                     G"
BASE COUNT         1203 a         2308 c         2406 g         1146 t
ORIGIN      
        1 gcgggctctc cttgcgtggg ggctccgtgc catcacagcc cgagccaggc ctgcaccgcc
       61 tcggcgtcga gcgccatgga cgtgaactgc accgccccgg gcttcccgga cgcggcatat
      121 gcgtcgaaca ccttgcccat cggaaagaaa gggtgaaccc gccagaaaga caggcggcgc
      181 ggggcgcaga gcgcggccgc ggtgaccaga tctcctgccc gacgcaggaa cggcacgttc
      241 aatttctcca ggaaatgatc gtcggtcatc tcgaacccgg ccaggtcgat cgcggcggca
      301 tccacccggc catccagggc gcacgccagg acggcccaga ggcccgcctc accaactccg
      361 accacttgca cgcgtgagac accctcctgc gcccggagga acgcgagcgc tgtcaggata
      421 tcctgcaccc ggttcccaat atcagtgcgg ttgtaggtgg tgaagtactt gatctcggcg
      481 atctccttct cccgcgtctc cggggctttc gagtcgcccg tcaggaaggt gtcgatcaga
      541 agcacgtgcc ggtcttcgcg cgagagctgc ccgaccaggc gcgaggtgat cccggggcca
      601 tccgcggcgc ggatcccttc tggcgtgacg aggagcacgc ccggcgccga acccttctga
      661 cggcgggacc ggtgtaggcg cgcgggcacc cggtcgccgg cgcccttccg ccccaggatg
      721 aggcgctggg cccgcgtgga tccgcccgcc ggggtggcgg tgacgagctc cggggccggc
      781 gcggcggcgc ccaggctatg gctcaacagc gggagatagg tctctctgaa ttggcgcgcc
      841 gtctcctgat cgcgtggcca gtgcgcggcc agctgcttct cgctgcgctc gaccaggtag
      901 cgcgtgatcc cctcctgatc ggcggcaccc tcgggaagct ggccatcggg gaaggtgagc
      961 aacaccttgg gatcaggcaa gtcgattggc tgctccttca gctgatcggg gtcgttgatg
     1021 cccaggagcc agcggcccat ccaggcatac accgcctcgc ggctttgcag gttgtagttg
     1081 tgcggagcgt cgaagcggag ggcatgcact ttctcttcgg cgcccatcag ccggtacacc
     1141 gcctggatcg cggggaactc ctcctcgagg gtgttgcgcg tccagtcgcc ggtcgccgcc
     1201 accaggagca gcggccgggg ggccatcagc gcgccgatct cgacgttggt gcactccact
     1261 cggaggttgg gcggattctc gcagaggcag cctccctgca tgtgcagcga gatcatgttg
     1321 accggcgctg ccaccttcac ccggtcgtca acagcggaga ggaggaatgt ctgcgtgcca
     1381 cccccggaag cccccgtgca cgccaggcgt tccggatcga ccatcggcag cgactccagg
     1441 taatcgaggg cgcgaatgct attccacagt tggaggccgc cgaggctgat gccccacagc
     1501 gcctcgcggt cgccaccgaa ggtgtggggg agctggcggg tgtcgttgta gccgatcatg
     1561 tcgtaggcga aggcgacaca gcccatgcgc gcgaaggtga tgcaccgcgc cggaatcgat
     1621 ccccgctcct gatgctcaat gcggccggtt ttccagtgcc cgtgcgggtt ggcgatgccg
     1681 ggaaagggcc cttgcttccc tttgggcagg tagaggtttc cgcccaggta gaagccgggg
     1741 agcgtctgga tggcgacgcg gcggatcaca taatcatcgc cctctatctc tcccgtcacg
     1801 atgggatcca gcgggcagcg ctccggcatg ggcgagaggc cagcgctggc gaggatgtgc
     1861 gccctcaact cgcgcgcacg cgcctcccac tcttctcgtg actcgaattg gggcgcgggg
     1921 aagtgctggt tcaggtggcg aacgaccgcc gcgcgacggt caaactcaac ggctgcttct
     1981 gtcatcgcga ttctctcccg tatcgccgcc ggccaggaca gacaagctgc caccagcagg
     2041 agaagtgttc tgatgcggat gcgcgacgcg cgcatgggct ttccgtcctc ctcccgagca
     2101 gcggggttct gcaaagaggt tcccacccag acggcctttc tcctgcgtgc cgggggcgtg
     2161 gagctcattc cagcaggcgg cggtagaggg cagtgtgccg ggcgagggct tccatgtacc
     2221 gcgcgtgccg ggcggcatcg gggtggtaga cctttccgaa ggcgaagggg gcgctctgga
     2281 tccctcggat cattccgagg gcttcgagcg ccatgagcgc ggcgccgcgc cctgaagcct
     2341 gcttctcgcg ggatgccacg accggtcggt tcagcacgtc ggcgatgatc tgcatccaga
     2401 ggggcgaata gagcagggcg ccgccggtgg cgatgacgcg gtagtctggc cccgcgtcca
     2461 ggcggtcgcg caggcgctcg tggatcagag cgaaccggta ggccaccgac tccaggccgg
     2521 cgcggatgat ctcgtggggc gaggtgtgcg ccgagaggcc ggcaatcacc gcacgggcat
     2581 caccgcgcca ccccgggctg cgctcgccca tgaggaaggg gagaaccgtc agcccgtggg
     2641 catctggctc catcctgctc agggcttcct cgaggcgttc gcgctggcca acgcgcagcg
     2701 tgcgggtgag ccacgtgtag aagttcccgc cgttgctcag cgcgcctccc agaagaaaac
     2761 ggcgctcgtc cagcaagtag gcccagagcc cttgcggggg tgtgggaccg ccggtggggc
     2821 agaggacgcg catggccccg ctggtgccca ccatgatcgc tacccggcgc cggctcaccg
     2881 cgccgctccc gacattgctg cacgcgccat cgcccaacgg cgggaaccac ggcacatcgc
     2941 ttaggttggg caggacacgg gcaaactccg gacgcagtcc tacggaagcc ctggagtgcg
     3001 cgaccggcgt gctgagctgc tcctcctcga tggggagtgc cgccagtacc tcggggtccc
     3061 acctcagacg acccagatca aataggcccg tgccggaggc catggagtgc gagcacgccg
     3121 cctccccgaa aagccgcagg aaaaggtact ccccgattga catccagcgc tggctgcggc
     3181 ggaagctctc cggctgggtg cgcgcgatcc agaggatgcg cgggcccaca taggaggtgt
     3241 gcagcgggca cccggtgcgt gcgtaaaccg ccggggcatc caggcgctgg cgcaacgcct
     3301 ccacctcggc cacgctccgc gtgtcggacc agaggtagat gggcgtggtc gcccgtccat
     3361 tggctcccac gcccaggatc gagtgccagt aggtgcagac gcccacgccg acgatccggc
     3421 gtgcctggga cccggcacgc tccaccgcct cggcagcgca ccgaacggcg cgctggacca
     3481 cctcctcggg gtgcatctcc gcgcccccgt cggccgtggt atggacgcgc tgggggcgcg
     3541 agctctccgc gcctggaacg ggcctgccct gggaggtgta gagcgccgcc cgtgtggacg
     3601 atgtgccgat atcaagcgag aggacgaggc caggccccgc gtcgctcata gcgtcgttcc
     3661 ctgccgcttg agcaccgaga ccggccactg cgcgcggtaa gcctgagtgc caaaccgctc
     3721 caggtcatcg aacaggaaga acatcccacc gcagcgccag atgttgccgg cggcgaggaa
     3781 atcggccacc ccgcggcgca gtgaggtgtc tcggggcagc gtggccgggt cgtagtcatc
     3841 gcgcaggaag acgaaccaca ggccgtccct gccgctggag gtgaccgggt ggagatacag
     3901 ctcgtgctgg taggccagca gattctccat ccgcggctcg atctcctcaa actgcgtgtt
     3961 gaagatccag gagacgcagg tgatcgcgcg ggtcggcttg tccgggaagt agcggcggaa
     4021 gaacgcggcg gcccggcgca tcgaatcgcc gcagcgatcc agggtcatcc ccccgcccgc
     4081 tgggatatgc atttcgagga tcgggtcgcc cgtcttcagc acgcactccc actccgcctt
     4141 cgggagcgtc acccgagtgc gcactgccat gccgtaaggc gagatcggat agccggtcac
     4201 cgaatcttcg tcctcggtga gcatcgcggt ccacccgcgc gcgatatcca tgcatcccgc
     4261 cgtaccatct acatggcctt tgccgttata gcgcacgcca tccggggcca gggccagcgt
     4321 ctcgccagtc ttgcggcttc ggaagacgcg gatcccctgc cggaatggct cgatcttata
     4381 ctcaaagcgc ccgagacgga agagcttgcc ggcaggatag tggcgcatcc agaagagagt
     4441 gtttactgtc acgcccagga ggccgcccgt catgcgccgg tagtttccgg ccacgcacga
     4501 tacctgagag agcgtctcgc gggtcacatc ctcgggcacg tccatggatg cgtgcagccg
     4561 gcgcacggtt gggaccatgc ccatcacggc caggaggtag aagacgccgc gcagctctcc
     4621 caaaacgccc tgcaacgaag gccagctccg caggaaggcc tgatcggtgt aatcgaggtg
     4681 cgtgaagagg agacgatggc agtgccacgc cagcgcgcac aagccgcgat ctgccgcgat
     4741 ccggcgcgcc gcctcatcca agagcgcgtc tacctcaggg ccgaagccac accactcccg
     4801 gttctcccga tactcttccg gtgtcaggaa ggaagggagc ccttcgccca gtgatcccag
     4861 agattcttcc cagtggggct ggagggcctc cagggactgg gtttcattca accgcttcaa
     4921 cagctcatcc agatgcatct ttagccgctc ccattctgcc gggttccgga cgcggccggc
     4981 tccgctccca tctgcccgcg acgctcttcc accaggcgct tgaagaggct cgcacgcatc
     5041 ccgcgccttc cgcttctctg ctcgggcaga cagaagttct attcacatcg tgccgcctcg
     5101 atcccttgtc gctgtgatcc agggggcaag gcgggcggct atggtggcag cgttcgggaa
     5161 cgccgctaca tccagtagac gacctcgggg gtgttgcgcg gcaccggcgg ggtggtcccg
     5221 gccgggtggc caacgataat cggagcgatc gccttgtagc tctccggaac gcccagctcc
     5281 gccttcaccg cgggcgggtc gaagaactcc tgggcgaagc cgatccagca ggtgcccagg
     5341 ttcatcgccg cggcggcaag catcaggttc tgagcggcca gggcgcaatc gatctccgga
     5401 agcaggctcg ctggcttgcc gtagatcacc actagcgcag gcgcgccgtg gaagacgttg
     5461 tatttcggat cggtgatcat gtcgcgatag cgctccagct ccggcatctg atcgagagcg
     5521 gggagcagct tcgccttcac ccggtctcca tactccttca gcctctccac gccggtgatc
     5581 acgccgaagg cccacggctg ggtgttcgtg gcgctgggcg cctgaatggc ggcgtggatc
     5641 agcttctcga tcgtggcccg atccaacggg gtgggagtgt aggaccgaac cgaacgacgc
     5701 gcggtaagga cgtccatgaa ctccatgtga gggctcctct ctcatcagga agcggccccg
     5761 gaacggccgg ccggtggccg cgctggaaga tctattcgcg ccggccgcga tgctccccta
     5821 gcatttggaa gaatcctccc gcgccgccca tgccatccga agagtgggag aggagagtga
     5881 gcgggattac ataacaaggg aatgatgtag gggggctgga ggcgggatgc gcgttcccgg
     5941 cagacggcct caatcgagga gtgtgcggtg agggaaagag cggccgggtt ggttctcggc
     6001 cgcttcggga gtgcgtgacg atgtcggagt ttccgcgtac catggtcggg ggcgtctcga
     6061 tgccccgtct gatctgtggc agcaactggt tcttgggcta ctcgcacacc agcagggcga
     6121 aagaccgact gatcaaggag ctgtttgaca cgcctgccaa gatggcgcgc gtggtggaag
     6181 tgtttgcgcg ccgcggctgc aacgctttca tgagcatgca gagccagttc gtcgccgagg
     6241 cgctgcgcga ggtggagcag cgcgtgggcg tgccgatgct gtgggtgacg acgcccacgt
     6301 acaaagagcc gggcaatccc gacacctgga aggcatccgt ggatgaggcc cgggagctcg
     6361 gggcgacctt ctgctttccc caccaatgcg tcacggaccc gcgcatcgac cgggtgaacc
     6421 gctgcctgag cccgcagctg attgaacatc tggcctacgt gcgggaagcg gggatggtgc
     6481 cagggctctc cagccatgcc ccggaggcga tcacgtgctc ggatgcgtgc ggggcggatg
     6541 tggagaccta catcaccccc tacaacgcgg cgggcttcct ctgccaggtg gagaccgact
     6601 ggatccagag gatcatccac agcgcgaaga agccggtgat gacgatcaag ccgcttgcgg
     6661 ccggccggct gctcccgccg acgggcctct ccttcgtctg gagcactctt cgcgactgtg
     6721 atctggtcac catcggcacg atgagcacct atgaggccga ggaagtgatc gagattagcc
     6781 tggcctgcct cgagcagcgc aaggctgagg tggagctgca gtacacccgg agcaagagca
     6841 cgctggtcgg gtgaggagtg ggaccccggt gggggagctg cccagcgtca catttccatc
     6901 acgctctcac ggcccgctta tcggagggac gatccacgcc gaaggggata ggaagcaggc
     6961 gactaccggc caggcgagcg aacaatcgga tggtgcggga agtcctaacg gtggggaggg
     7021 ccgcgatgat gtgccgctat gggatcggca ataggcgcgc tgc
//
LOCUS       DAOSCV010000086         7019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6907_length_7019_cov_3.408960, whole genome shotgun sequence.
ACCESSION   DAOSCV010000086 DAOSCV010000000
VERSION     DAOSCV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6907_length_7019_cov_3.408960"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1795
                     /gene="rny"
                     /locus_tag="PLY56_03400"
     CDS             272..1795
                     /gene="rny"
                     /locus_tag="PLY56_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ20556.1"
                     /translation="MGKWELVQVSLIIFFLFLVYFRALMNLRLKEREADRILEEAEEQ
                     KEAKKREAILEAREEIQRLRTEAEREIRERRADLQRSERRISQREENIERRQAALERR
                     ERQTQQKEQEANRLHEEASRLVEAQRQELERVAQLPAEEARQIILRRVEEECQLDAAR
                     LVKAIEAEAIEEAEQRARKVITLAIQKCAVDQTAETTVSVVTLPNDDMKGRIIGREGR
                     NIRAFETLTGVDLIIDDTPEAVVISAFDVVRREIARIALENLVLDGRIHPARIEEMVQ
                     KARAQVEQEIKMAGERAILDTGLSGVDPELIKILGRLRYRTSYGQNVLKHSMEVAYLA
                     AAMASELGLDPLLAKRAGLFHDLGKAVDFETDGPHATIGADLARKHGEPREVVQAIAA
                     HHADVDQDSAYAVLVQAADAISAARPGARREGLETYVKRLETLEQLGDSFDGVEKTFA
                     VQAGREIRVMVKPDRVTDEGAAKLARDIVKRIESELEYPGQIKVTVIRETRMVEYAK"
     gene            complement(2110..4380)
                     /locus_tag="PLY56_03405"
     CDS             complement(2110..4380)
                     /locus_tag="PLY56_03405"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HOJ20557.1"
                     /translation="MRELDVMPRCEPETLRFGEIPKCRYSGTLRAEIEAGRITPQAAR
                     EMLETMFLVRAFEALIVDMKNNKFSPAPGFKFIGASHLSIGQEAVAVGVMAATRGDDY
                     ITSTHRGHGHSIAKGVYALRAMDAEQLKNWLGDPNAQGTREELLQRALKTHLYKTVAE
                     LFGKEDGYCRGRGGGMHIADFHLGHLGANAIVGGSSAIAAGAALAAQRLGEGKICVGI
                     VGDGATNNGIFHEACNFAAQDQFKKGIPLLFVIENNQYGMTGQQAGEVTGVDYLARRG
                     AAYNKEMMHAEVVDGMNVLAVWDAVLRGAALCRSDQGPVLLEALCYRYYGHSLSDNRT
                     TYRTPEEEEAWRNNDPIARFSREVVAAGVMTQAEVAAMEQACTDAMRQAAIDAAASAD
                     PDPAQIEEGLFSNTMCDEVPARFKHTKLVGELRRARRDGQGRILYRHAIAEALAEEML
                     RDERVIFYGEDVADYGGAFQATVGLLAAFGRDRVFNAPISEACIVGTAVGAAMVGLRP
                     VCEIMYIDFMGLTMDQTGNQAAKVRYMFGGKALVPMVVRTTVGGGKGYAGQHSQSLEA
                     MCTMFPGLKVVMPSTAYDAKGLLKTAIRDNDPVIFIEHQLLYTERGVVPEEEYLVPFG
                     QAAIRREGKDLTIVAYSRMVDVALEAAEMLAAEGIQAEVIDPRTLIPFDYDAVTASVE
                     KTGRALLLCQAPETGCFAEHIAYQTQKRAWKSLKAPVRIVAAYNVPPPMSAVLEMENL
                     PDAAKVVRNARELLAE"
     gene            5129..6373
                     /locus_tag="PLY56_03410"
     CDS             5129..6373
                     /locus_tag="PLY56_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ20558.1"
                     /translation="MRALLALLFVGMGIAAGAIYHLSRPFVTPGAGLSEQVREIRIAA
                     TNPRDAFPNKSRLYILCLGIDANHTSKGIMHTKNARSDTIFVVSLDLDAKRVGMLSVP
                     RDTRVEFAYGRGTDKINAAHAIGGVPLAKATVEQFLGIEIDHYVVIKMYGTRNMIDAM
                     GGVVIDVEKDMDYDDNWGNLHIHLKKGRQRLNGEQAVGYVRFRHDAESDFGRMRRQQQ
                     LVKAVARQLMTPSTLLNLDKLVDIAMENTITDLSRSQILALAHLFYNVPPEKIYTGTI
                     AGYDYRGSNGIWYLDPSESKKEALVGWLLRGEEWYRNGLITVRVHNASGINGVASTLR
                     DILADQGYNAIIGRSERGERQPLTRVVDLTGKTEAAQEIARLLPNARVDPPTDDEKPE
                     PSGPDILVKIGTDCARAIGRSL"
     gene            6446..>7019
                     /locus_tag="PLY56_03415"
     CDS             6446..>7019
                     /locus_tag="PLY56_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018633058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ20559.1"
                     /translation="MPSRTSQVPLPLDVILQGDCRQVLQELPEECVDLVFADPPYYLQ
                     LGEETLLRPNLTRVDGVDDAWDSFGSFAEYDAFTAEWLRACRRVMKETATLWVIGTYH
                     NIHRVGALLQDTGYWILNEIVWIKRNPMPNFRGVRFTNAHETLLWAKKSERQKRYTFN
                     YQLMKELNGGTQMRSDWLIPVWRGEERLQAD"
BASE COUNT         1282 a         2179 c         2331 g         1227 t
ORIGIN      
        1 ttcctgcaaa ggcggggctt tgcatacgaa acgatctcaa gagtactgag tatggtcctt
       61 cctcccgagt gagtggcgcc ggacgtggaa gccgggcacg cattgagccg atagatggtt
      121 gtgcaagcag ggtgtacgcg cgggacgcat cctggatgcg ccctgcgggt aggcatccag
      181 agcggccgac tgcacacttt gccatagccc ttgctcgctc tccagagcgc tgcctgcctg
      241 ggccgggacg gaaggttagg agagtatagc aatgggcaag tgggaactgg tccaggtatc
      301 tctcattatc ttcttcctct ttctggtcta ttttcgagcg ttgatgaacc ttcgcctaaa
      361 ggagcgagag gcggatcgga tcctagagga agcggaagag cagaaagaag cgaaaaagcg
      421 ggaggcgatc ctcgaggcac gcgaggagat tcagcgccta cgcactgagg cggagcgcga
      481 aatccgcgag cgccgcgccg atctgcagcg ctcggaacgg cggattagcc agcgtgagga
      541 gaatattgaa cgccgccagg ccgcgttgga gcgccgcgag cgccagaccc aacagaaaga
      601 gcaagaagca aaccgcctgc acgaggaggc aagccgcctg gtagaggctc agcgccagga
      661 gttggagcgc gtggctcagc ttcctgccga ggaagcccgt caaatcatcc ttcgccgcgt
      721 ggaagaggag tgccagctcg acgcggcccg cctggtgaaa gcgattgagg ccgaggcgat
      781 tgaagaggcc gagcagcgcg cgcgcaaggt gatcaccctg gcgatccaga agtgcgccgt
      841 ggaccagacc gcggagacca cggtctcagt cgtcacgctc cccaacgacg acatgaaggg
      901 gcgcatcatc ggccgcgagg gccgcaacat ccgcgccttt gaaacgctca ccggtgtcga
      961 cctgatcatc gacgacaccc ccgaggcggt cgtcatctca gccttcgatg tggtccggcg
     1021 ggagatcgcg cgcattgccc tcgaaaacct ggtgcttgac ggccggatcc atcccgcgcg
     1081 cattgaggag atggtgcaga aggcgcgcgc ccaggtagag caggagatca agatggccgg
     1141 cgagcgcgcc atcctggata cgggactctc tggcgtggat ccggagctga tcaagatcct
     1201 cggccggctg cgctaccgca ccagctacgg gcaaaacgtc ttgaagcact cgatggaggt
     1261 cgcctatctg gcggccgcca tggcctctga actgggcctg gatcctctcc tcgccaagcg
     1321 ggccggcctg tttcacgacc tgggcaaggc ggtcgatttc gagacggatg gcccgcatgc
     1381 gacgatcggt gccgacctcg cacgcaagca tggcgagccg cgcgaggtgg tccaggcgat
     1441 tgccgcgcac cacgccgatg tggaccagga cagcgcctac gccgttctgg tccaggccgc
     1501 cgatgccatc tccgccgcgc gtcccggagc ccgtcgcgag ggcctggaga cctacgtcaa
     1561 gcgtctggaa actctggagc agctgggcga ttcgttcgat ggggtggaga agaccttcgc
     1621 cgtgcaggcc ggccgggaga ttcgggtgat ggtgaagccg gaccgggtga ctgatgaggg
     1681 cgccgccaaa ctcgcgcgag acatcgtgaa gcgcatcgaa tccgagctgg aatacccggg
     1741 tcagatcaag gtgaccgtca tccgtgagac gcgcatggtc gagtacgcga agtagcccgg
     1801 cctaatcccc ggctccttcc ctgtcctccg gctgcacgcc ggccccaggg aaggggggct
     1861 gccgcggggg gatgaggaga gctccggatg gggcgatgcc ctgcaaggct gggtcgcgtg
     1921 gcccgaagga cctttcttgg gcgtgcgcgg ttggctggat gcgaatatgt gcgggggcaa
     1981 acctttagtg ttcgccttgt ttccagtgag gcagggcgaa cagtgaatgt tcgctgctcg
     2041 cccgtattgt gaaggggaac cgccggggcg tgcgttagac gccccggcgg cctcagttta
     2101 ccgggtgggt tactcggcga gaagctcgcg cgcgttgcgc accaccttgg ccgcgtccgg
     2161 caggttctcc atctcgagaa cggcggacat tggcggcggc acgttgtagg cggcgacgat
     2221 gcgcacgggg gccttgaggc tcttccatgc gcgcttctgc gtctggtagg cgatatgctc
     2281 cgcgaagcag cccgtctctg gcgcctggca gaggagcagc gcccggcccg tcttctcgac
     2341 cgatgccgtc accgcgtcgt agtcgaaggg gatgagcgtg cgcgggtcga tcacctcggc
     2401 ctggatcccc tcggcggcga gcatctcggc ggcttcgagg gccacatcga ccatgcggct
     2461 gtaggcgaca atcgtgaggt ctttcccctc gcggcggatc gccgcctgcc cgaacggcac
     2521 caggtactct tcctccggca cgactcccct ctcggtgtag agaagctggt gctcgatgaa
     2581 gatcaccggg tcgttatcgc gaatggcggt cttgagcagg cccttggcgt cataggctgt
     2641 cgagggcatg accaccttca ggccggggaa catggtgcac atcgcctcga ggctctgcga
     2701 gtgctggcca gcatacccct tgccgccacc cacggtggtg cgcacgacca tcggcaccag
     2761 cgccttgccg ccaaacatgt agcgcacttt ggccgcctgg ttacccgtct ggtccatggt
     2821 gaggcccatg aagtcgatgt acatgatctc gcagacgggc cgcaagccga ccatcgcggc
     2881 gccaacggcc gtgccgacga tgcacgcctc ggagataggc gcgttgaaga cgcggtcgcg
     2941 gccgaaagcg gcgagaaggc caaccgtcgc ctggaacgcg ccgccgtaat cggcgacatc
     3001 ctcgccatag aagatgacgc gctcatcgcg gagcatctct tcggcgagcg cctcggcgat
     3061 ggcgtggcga tagaggatac gaccctgacc gtcgcggcgt gcgcgacgca gctcacccac
     3121 gagcttggtg tgcttgaagc gggccggcac ctcgtcgcac atcgtgttgg agaaaagccc
     3181 ctcctcgatc tgcgccgggt ctgggtcggc actcgcggcg gcgtcgatgg cggcctggcg
     3241 catcgcgtcg gtgcacgcct gctccatggc ggccacctcg gcctgggtca tcacgccggc
     3301 agccaccacc tcccgggaga atcgggcgat gggatcgttg ttgcgccagg cctcctcctc
     3361 ctcgggcgtg cggtaggtgg tgcggttatc gctgagcgag tggccgtagt agcgatagca
     3421 gagcgcctcg aggagcacgg gaccctggtc actccggcaa agagcggccc cgcgcaagac
     3481 agcatcccaa accgcgagga cgttcatgcc atccacgacc tcggcgtgca tcatttcctt
     3541 gttgtaggcg gcgccgcgcc gggcaagata gtcgacgccg gtcacttcgc ccgcctgctg
     3601 gccggtcatg ccgtactggt tgttctcgat gacgaagagg agggggattc ccttcttgaa
     3661 ctgatcctgg gcggcgaagt tgcacgcttc gtggaagatg ccgttgttcg tggcgccatc
     3721 accgacgatg ccgacgcaga tcttcccctc gcccaggcgc tgtgcggcga gcgccgcgcc
     3781 ggcggcaatg gcgctgctgc cacccacgat ggcgttcgcg cccaggtgac caaggtggaa
     3841 atcggcgatg tgcatgccgc cgccgcgacc gcgacagtag ccgtcttcct tgccgaacag
     3901 ctccgccacg gtcttataga ggtgggtctt cagggcgcgc tgtagcagct cctcccgggt
     3961 gccctgagca ttcgggtcac caagccagtt tttgagctgc tcggcatcca tggcacgcag
     4021 cgcgtagacg cctttcgcga tggagtggcc gtgcccgcgg tgggtgctgg tgatgtaatc
     4081 atcgccgcgg gtcgcggcca tcacgccgac ggccactgcc tcctgaccga tggagaggtg
     4141 gctggcgccg ataaacttga acccgggcgc cggcgagaac ttgttgttct tcatgtccac
     4201 gatgagcgcc tcgaaggcgc gcaccaggaa catcgtctcc agcatctcgc gggctgcttg
     4261 cggggtgatc cgccccgcct caatctcagc gcgcagcgtc cctgaatagc ggcacttggg
     4321 tatctctcca aaccgaagtg tctccggttc acaacgcggc attacgtcca attcacgcat
     4381 cagacactct cccactcccg ataaagcgtg acagccaggg cgtgtgtcgg gaggcgcaac
     4441 gcccgtcact cacccgcgct cctcatcggg acagcgcgga gaagccgtgc cgcccgtgtc
     4501 ggcccggctc aaccggccgg gtggcaagtg agttcttcga agtattcacg gggactactt
     4561 ccgacaggtt cgttcgacaa aggtgcgcat tctcctggcg cgatcctacc ggcgatcctc
     4621 gcgcccatga gggagatccg gttccatctc gcgatgccag gcgggcgggg gcaggctcgc
     4681 ggaggcccgc agggcctttg cgggaaagca gcgcggggtt tcaggtcggc gcttgggcaa
     4741 gcatcgcatg gccctatttt gacgccttgc tgccacggtg atataatagg ggccggcgga
     4801 tcggcactgg ccactctgac gcatgtcttc cgatgggagc gtcggcacgg gcacgggccc
     4861 ttcgctcacc cggtcgcacc tcgggcgttt ctgaggcgcg gggtggctga acatcgcgag
     4921 cgaaaggacc cccgcggcgg ggcatatgaa tcaacgcccc ggctttaccg atgggcacct
     4981 acgtgagggg tacctcggaa acgtgggctg gcaagagcct tctcgacgcc gcgctcaggc
     5041 ctgagcgggg tatgtccata gcaaaatgat ggagagaccg ataccaccgc gtatgttgca
     5101 tccacgccgg cggcggaaaa gccggctcgt gcgcgccctc ctcgccctcc tgtttgtcgg
     5161 gatgggtatt gcggcgggag ccatttacca cctgagccgg cccttcgtca cgcccggagc
     5221 gggcctgagc gagcaagttc gcgagatccg gatcgcagcc acaaacccgc gtgatgcctt
     5281 ccccaacaag agccggctct acatcctatg cctcgggatc gatgccaacc acacgtcgaa
     5341 gggcatcatg cacaccaaga atgcccgttc cgacaccatc ttcgtggtta gcctggatct
     5401 ggatgcgaag cgcgtgggta tgctctcggt gccgcgtgat acgcgcgtcg agtttgccta
     5461 tggtcggggc accgataaga tcaacgcggc gcacgccatc ggcggcgttc cccttgccaa
     5521 ggcgaccgtg gagcagtttc tcggcatcga aatcgaccat tacgtcgtca tcaagatgta
     5581 tggcaccaga aacatgatcg atgccatggg tggcgtggtg atcgatgtcg agaaggatat
     5641 ggattatgac gacaactggg gcaacctcca tatccacctg aagaagggcc gacagcgcct
     5701 gaatggggag caggcggttg gctacgtgcg ttttcgccac gatgcggagt cggatttcgg
     5761 gcgtatgcgc cgccagcaac agcttgtcaa ggccgttgcc cggcagttga tgacgccctc
     5821 cacgctgctc aacctggata agctggtgga tatcgcgatg gagaatacca tcacggacct
     5881 cagccgctcc cagatcctcg cgctggcgca tctcttctac aatgtgcccc cggagaagat
     5941 ctacaccggg accatcgccg gatacgatta ccgtggcagc aacggcatct ggtaccttga
     6001 ccccagcgag agcaagaagg aggcgctcgt agggtggctc cttcgcggtg aggagtggta
     6061 tcggaatggg ctgatcacgg tgcgcgtcca caacgcgtcc ggcatcaacg gggtggcttc
     6121 gacattgcgc gatatcctgg ccgaccaggg ctacaacgcg atcatcggcc ggtcggagcg
     6181 cggggagcgg cagcccctca cccgcgttgt cgacctcact ggcaagacag aggccgctca
     6241 ggagatcgcc cgtctcctcc cgaacgcccg tgtggatccg cccaccgatg acgaaaagcc
     6301 ggagcccagc ggccccgaca tcctggtcaa gattggcacc gattgcgcga gggcgattgg
     6361 tcgctccttg tgatcacgag acgggcacgc cacggatgca cgatgagagg gggccaaacc
     6421 ccggcgagtg gaaggagtag cgcctttgcc gagccgcacc tcgcaagtgc ccctcccgct
     6481 agatgtgatc ctccagggcg attgccggca ggtgctccag gaactccccg aggagtgcgt
     6541 ggacctggtc ttcgccgatc cgccttacta cttgcagctg ggggaggaga cgctgctgcg
     6601 cccgaacctg actcgagtgg atggcgtgga tgatgcctgg gactcgttcg ggagcttcgc
     6661 ggagtacgac gccttcaccg cggagtggct gcgcgcttgc cgcagggtga tgaaggagac
     6721 cgcgacgctc tgggtgatcg gcacttacca caacatccat cgcgtgggtg ccctccttca
     6781 ggatactggc tattggattc tgaacgagat cgtctggatc aagcggaacc cgatgccgaa
     6841 tttccgcggg gtgcgcttca cgaatgccca tgagacgctg ctttgggcca aaaagtcgga
     6901 acggcagaag cgctacacct tcaactatca gctgatgaag gagctgaacg gtggcacgca
     6961 gatgcgcagt gactggctga tcccggtctg gcggggggaa gagcgcctgc aagccgatg
//
LOCUS       DAOSCV010000087         7010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6921_length_7010_cov_3.549101, whole genome shotgun sequence.
ACCESSION   DAOSCV010000087 DAOSCV010000000
VERSION     DAOSCV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6921_length_7010_cov_3.549101"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="PLY56_03420"
     CDS             <1..1330
                     /locus_tag="PLY56_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20560.1"
                     /translation="EIGATSALVVPMRARERLLGIIALFMAESGRSYDGARVTLATTL
                     AERAAGALENARLYEEATAANAAKDQFLAVLSHELRNPLAPVLAGAAVLKRVAPQEER
                     VQRTVAIIERNVKLQARLINDLLDLSRVKRGKMRLQRVPTLLDNVVQAAVQTQQRDAE
                     EAGLDLQVQLQPGLWVHGDPDRLQQVVMNLLSNAIKFTPSPGQVRVILEKSDSMARIT
                     VSDTGIGISPDLLPQLFGIFQQGEVAGRRAAGLGIGLSLVKSITELHGGNVRVESDGP
                     GTGSRFTVELPLIPSPGETSASQPPQHPTEARVLLIEDNPDTCALLKEELQALGHTVR
                     AVATGEAALEWLQHEHPEVIVTDIGLPGIDGYEFLRRARELPSMRAVPAFAITGYDAP
                     PDLERAREAGFNGYLTKPVDIAILDRCIRQRMAPEEGPAAAGQVEERPNP"
     gene            1639..2895
                     /locus_tag="PLY56_03425"
     CDS             1639..2895
                     /locus_tag="PLY56_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOJ20561.1"
                     /translation="MRQKSSHGVLLSVVASASALALLIPLAGAQAGPIASGVRMDIEW
                     RHRVEARGNNDFDAAQDDGQTGYLTRFRLGMGARLSPGLTAYAQLQDVRRSGDLSQRV
                     TNLTTLHQGYLDFPGGKEGTSLRVGRQEIAFGEKRLVSDSGWSNSARTFDAVRLTHRA
                     GPYTLDLFSANVVTKGYQPRNNPPQGSFHGLYMTSGSGKKTVKDAYALLRWRDGNLWT
                     YGGRVRTPAAPGRVDWMAELAWQTGRQQGRKHQAHAGVLSAGYTLTAPWTPRIGVEYA
                     YSPGDSNPDDPVVKTFDPLYPTTHPYYGIMDYFAWRNLRNLRASLSVNPLPRLRVAVD
                     YHDMRLASAEDGWYGSNALRMQDKSGRSGRHVGREIDLQVDYRLGPRTALSAGYGHFF
                     AGSYITRQKAAATDSDWGYLQATYSY"
     gene            complement(2974..5427)
                     /locus_tag="PLY56_03430"
     CDS             complement(2974..5427)
                     /locus_tag="PLY56_03430"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ20562.1"
                     /translation="MRYLLQGCAILLWMTMAAHAAPLPLVVEGRSQYAIYHAPDAPDT
                     VKMAATELRDYIRQATGASLPISQQPREPMLSLGANAAARAAGLSTQGIPLEGFRIVT
                     RGRNLFILGPDTAEGERTPGGGTSAGTRNGVYAFLEEYLGVRWLMPGPHGDYVPRAAS
                     VTIPEMDRTDAPFFLNRRVPYTQERRAEVKQWWARQRLGWSLTLSHGHNWHSIPPEAF
                     DQHPDWFAEIGGKRIPPRGWYKLCVTNQGLIRAFAEGAIRRFDENPELTTYSLSPSDG
                     GGWCQCAACQALYEKDPNGDLSVTPAILTFYNEVAKLVARKYPNKLLAGYVYANYVFP
                     PSKPIRLEPNLFLVWAPSFDYGFTLYRPELQKQWDELVAQWRAVTDNISYYDLPNCVH
                     NGAGAPNPPGLKILKFLYPRLKQAKMKGVYVYGNPAWGHSGPMNYILARLAWNPDADI
                     DALFNEFCEKAYAEGAEEMKRFFHLLDAETERFFIANTKESYVLSEARMRDVYAKNFP
                     EMERLYRAAMAKVRDAEAKARLEMLGLNLSVLHWNLRQRKMLPQATASTFHMEDAAFF
                     ALLRKHSDSLALAPSAMSARPAVVERPLSVQVPAKLAHPSPVTPFLFRGDQHLVLRPN
                     GNEPVKVSFHSITTRGTLLHYALFAADGAEVTRGLVGPETPLVLENPASPYYHLYFSA
                     GHATFGLAVEGAAWAANGHMSDQGLHFLGKVTPVYFEVPQGVASFQLSLGATPPGETA
                     VATLYAPNGREVARFDCTTIPVDRKRITVPAGGAGFWKLVVEEAPTGVIDDVWILASP
                     EIPGFFSLAPDQALSVRPH"
     gene            complement(5556..6548)
                     /locus_tag="PLY56_03435"
     CDS             complement(5556..6548)
                     /locus_tag="PLY56_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20563.1"
                     /translation="MGVLVGRVILALVVLAVSEPAMAAESTLEYPLYRVTVAPLLDGK
                     IEGDPGWQGVPVATGFFRLGGGYTAAKQTEVRAAWDGRVIYIGIHCEEPDIAVIEPTG
                     KDGGELWAEDGVEIFVRPEGVAGFHQFIVNTAGARRAAGLSDGTLEWQAAASRSADAY
                     TLEVALPLALFGQPVQAGSVWRGNFCRNIFTTQSGGDRFTTWAPLAASFHEWERFARW
                     HFRAEPPDPAAVRPVEARLNGAYRAHLRAEAARLSRRGNEYLPVLRKAMRVPRFQDQA
                     ARLIGEWEEAARLRRMGRLAPAYELRRAVATSERLVERSFALKYDYLFWELFEE"
BASE COUNT         1211 a         2423 c         2215 g         1161 t
ORIGIN      
        1 cgagatcggc gctacctcag cactcgtggt ccccatgcgc gcgagagagc ggctactcgg
       61 catcatcgcc cttttcatgg cggaatcggg gcgctcctat gatggcgcca gagtgacgct
      121 ggccaccacc ctcgccgagc gcgctgccgg cgccctcgag aacgcgcgcc tctacgagga
      181 agccactgcg gccaacgcgg ccaaggacca gtttctggcg gtgctctcac acgagttgcg
      241 caaccccctg gcgcccgtcc tcgccggtgc ggcggtgctc aagcgcgtgg caccacagga
      301 agagcgggtg cagcgcaccg tggccattat cgagcgcaac gtcaagttgc aggcccggct
      361 gatcaacgat ctcctcgatc tctcgcgggt caagcgaggg aagatgcgcc tgcaacgcgt
      421 gccgacccta ctcgacaatg tcgttcaagc cgctgttcaa acccagcagc gcgatgcgga
      481 agaggccggc ctcgacctac aggtgcaact ccagccgggc ctgtgggtgc atggcgaccc
      541 ggatcggctg cagcaagtgg tgatgaacct cctctccaac gcgatcaagt tcaccccatc
      601 ccccggccag gtgcgcgtca tcctggagaa gtcggactcg atggcgcgga tcaccgtcag
      661 cgataccgga atcgggatca gccccgacct gctcccgcaa ctctttggca tctttcagca
      721 gggcgaggta gccgggcgca gggccgcggg cctgggcatc ggcctgtcgc tggtaaagtc
      781 gatcaccgag ctgcatggcg gaaacgtccg ggtcgagagt gacgggcccg gcacaggaag
      841 ccgcttcacc gtggaactgc cgttgatccc cagcccggga gagacctccg cctcgcagcc
      901 accgcagcac cctaccgagg cgcgcgtgct cctgattgaa gataatcccg acacctgcgc
      961 gcttctcaag gaggagctac aggccctcgg gcatacagta cgcgcggtag cgaccggcga
     1021 ggctgcgctc gagtggctcc agcatgagca tcccgaggtg atcgtcaccg atatcggctt
     1081 gcccgggatc gatggctacg agttcctgcg ccgtgcgcgc gagctcccct ccatgcgcgc
     1141 ggttcccgcc ttcgccatca ccggctacga tgcccctccg gatctggaac gcgcacgaga
     1201 ggccggcttt aatggctacc tcaccaagcc ggtggatatc gccattctag atcgctgcat
     1261 ccgacagcgg atggctccag aagagggccc cgcggccgcg gggcaggtag aggagaggcc
     1321 gaacccctga tgcagccgct tcttgtcgac tgaccggatg gcactgaagc gccgtgacgt
     1381 ctgctcccca agcacagcct ctaggcacgc ctagagccgc atgccgccta tgaatcatgg
     1441 cggacctcct ccagccgctc gcttagcatc cttttaacat tcacccggca ccgaaagaca
     1501 ggcgcgtgtg ctatactggg gagatgattt ggcctgttcc gcgtgtcctc cgcgcgccat
     1561 ccacacggga agatcccgcg ctccatcacg ccacatacgc ttacgccacg cggcaccagg
     1621 cggagaggag accgtcatat gagacagaag tccagccatg gagtgcttct gagcgtggtc
     1681 gccagtgcct ccgcgctcgc cctgttgata ccgctcgccg gcgcgcaagc cggccccatc
     1741 gcgtccggag tgcgcatgga tattgagtgg cgccaccgcg tggaggcacg aggcaataac
     1801 gactttgatg ccgcccagga cgatggccag accggctacc tcacccgctt tcgcctgggc
     1861 atgggcgccc ggctctcccc gggcctcact gcctacgcgc agttgcagga tgtccggcgt
     1921 tccggcgatc tgtcgcagcg cgtcacgaat ctcactacgc tccaccaggg ctacctggac
     1981 ttcccaggtg gcaaggaggg cacttccctg cgggtaggac gccaggagat cgcctttggt
     2041 gagaagcgcc tggtgagcga ctccgggtgg agcaactccg cgcgcacctt cgacgccgtg
     2101 cgcctcaccc accgcgcggg cccctacacc ctcgatctgt tcagtgccaa tgtcgtcacc
     2161 aagggctacc agcccaggaa caacccgccg cagggctctt ttcacggcct ctacatgacc
     2221 tccggcagcg gcaagaagac ggtcaaggat gcctacgccc tcctgcgctg gcgtgatggc
     2281 aacctctgga cctatggcgg ccgggtgcgc actccggccg cgcccgggcg ggtggattgg
     2341 atggcggagc tcgcctggca gacaggacgc cagcagggga ggaagcatca ggcgcacgct
     2401 ggggtcctca gcgccggcta tactctcacc gcgccctgga caccgcggat cggcgtggag
     2461 tatgcctaca gccccggcga tagcaacccg gacgaccctg tcgtcaagac cttcgatcct
     2521 ctctatccca ccacccatcc ctactatggg atcatggatt acttcgcctg gcgcaacctg
     2581 cgcaacctgc gcgcgtcgct ctccgtgaat cccctgcccc gcctgcgggt cgccgtcgat
     2641 taccacgaca tgcgtctcgc ctcggcggaa gatggctggt atggctccaa cgcgctgcgg
     2701 atgcaagaca agagcgggcg gtccgggaga cacgtcgggc gcgagatcga tctccaggta
     2761 gattaccggc tcggcccgcg aacggcgctc tccgccgggt acggccactt cttcgccggc
     2821 tcctacatca cccgacagaa agcggctgcc acggactcgg actggggcta cctgcaggcg
     2881 acctactctt actgacgagg ggttgatgtt caccgggccg agggcgggat gcggccacgc
     2941 atcccgccct tgcccatttc tctgcccgcc cggctagtgg gggcgaacac tcagcgcctg
     3001 gtcgggcgcc agggagaaga agccggggat ctctgggctc gccaggatcc agacgtcatc
     3061 gatcactccg gtgggcgcct cttcgacgac gagtttccag aagccggcgc caccggccgg
     3121 caccgtgatc cgcttccggt ccacggggat cgtggtgcaa tcaaaccggg ccacctcgcg
     3181 cccattcggg gcgtagaggg tggcaaccgc cgtctcacca ggaggtgttg cccccagcga
     3241 gagctggaac gaggccacac cctgaggcac ttcgaagtag accggcgtga cctttccgag
     3301 gaagtgcagc ccctggtcgc tcatatgccc gttcgcggcc cacgcagccc cttcgaccgc
     3361 gagcccgaac gtggcgtggc cggccgagaa gtagaggtgg tagtacggcg aggccggatt
     3421 ctcgagcacg agcggtgtct cagggcccac cagcccgcgg gttacctccg caccatccgc
     3481 ggcaaagagc gcgtagtgga gcaacgtgcc tcgcgtggtg atcgagtgga aggagacctt
     3541 caccggctcg ttcccattgg gccggagcac cagatgctgg tcgccccgga aaaggaacgg
     3601 agtgacaggc gaaggatgag ccagcttggc ggggacctga acggagagcg ggcgctccac
     3661 gacggcgggc ctggccgaca tggccgatgg cgcgagtgcg agtgagtcgg aatgcttcct
     3721 cagcagggcg aagaaagcgg catcctccat atggaaggta gacgccgttg cctgggggag
     3781 catcttccgc tggcgcaggt tccaatggag taccgagagg ttgagcccga gcatctccag
     3841 gcgcgccttc gcctccgcat cgcgcacctt cgccatagcg gcacggtaga ggcgctccat
     3901 ctccgggaag ttcttcgcgt agacgtcgcg catgcgcgcc tcgctgagga catagctttc
     3961 cttcgtattc gcgatgaaga agcgctccgt ctctgcatcg agcagatgga agaagcgctt
     4021 catctcctcg gcgccctcgg cgtaggcctt ctcgcagaac tcgttgaaaa gcgcgtcgat
     4081 atcggcatcc ggattccagg ccagccgggc cagaatgtag ttcatcggcc cgctgtgtcc
     4141 ccatgccgga ttgccgtaga cgtagacgcc tttcatcttc gcctgcttta gccgcggata
     4201 gaggaacttc aggatcttca gtcccggcgg gttgggcgcg cccgcaccgt tgtgcacgca
     4261 gttcggaagg tcataatagg agatgttgtc cgtcaccgcg cgccactgcg ccaccagttc
     4321 gtcccattgc ttttgcagct ccgggcggta gagggtgaag ccgtaatcga agcttggcgc
     4381 ccacaccagg aagaggttcg gctccaggcg gatcggcttg ctgggcggga agacgtagtt
     4441 cgcgtaaacg tagccggcca gcagcttgtt tgggtatttc cgggcgacca gtttcgccac
     4501 ctcgttgtag aaggtgagga tcgccggggt caccgagagg tcgccgtttg gatccttttc
     4561 gtacagtgcc tggcaggccg cgcactggca ccacccgccg ccatctgaag gcgagagcga
     4621 gtaggtcgtc agctccggat tctcatcgaa gcggcggata gccccctcgg cgaaagcacg
     4681 gatcaacccc tggttggtga cgcacagctt ataccagcct ctgggcggaa tccgcttccc
     4741 gccgatctcg gcgaaccagt cggggtgctg gtcgaacgcc tccggcggga tggagtgcca
     4801 gttgtgcccg tggctcaggg tgaggctcca ccccaggcgc tgccgggccc accactgctt
     4861 cacctcggcg cgcctttcct gggtgtaggg cacgcgccgg ttgaggaaga agggcgcgtc
     4921 ggtccggtcc atctccggga tggtgaccga ggccgcccgc ggcacgtagt cgccgtgagg
     4981 cccgggcatc agccaccgga cgcccaggta ctcctcgagg aaggcgtaga cgccgttgcg
     5041 ggtgccggcg ctcgtgccac caccgggcgt ccgctcgcct tcagcggtat ccgggcccag
     5101 aatgaagagg ttccggccgc gcgtgacgat gcgaaagcct tcgaggggga tcccctgggt
     5161 ggagaggcca gccgcccgtg ccgcggcatt ggcccccaga gagagcatcg gctcgcgcgg
     5221 ttgctggctg atcggaaggc tcgcccccgt cgcctggcgg atataatccc gcaactccgt
     5281 ggccgccatc ttcaccgtat ccggcgcgtc cggcgcatgg tagatcgcat actgcgagcg
     5341 cccttccact actagcggga ggggcgccgc atgcgccgcc atggtcatcc agagtagaat
     5401 cgcacagccc tgaagtagat acctcatctg cctcttattt gccccttctt ccacatgccg
     5461 gcaaccaacc gccgctcact cttctctgca ttccccctcg acgggcgttt tccctatcac
     5521 cggtcgctcg tcaaggcgag aggaccggtc gcgtgtcact cctcgaacag ctcccagaag
     5581 agatagtcgt acttcaaggc aaaggagcgc tccaccaggc gctcgctggt cgcgacggcc
     5641 cggcgaagct cgtacgctgg tgcgagacgg cccatgcgcc tgaggcgcgc tgcctcttcc
     5701 cactcgccga tgagccgagc cgcctggtcc tggaagcggg ggactcgcat cgctttgcgc
     5761 agcaccggaa ggtactcgtt tccgcgcctg gagagacgcg ccgcctcggc gcgcaaatga
     5821 gcgcggtagg cgccattcag acgggcttcc acagggcgca ccgccgcagg atcgggcggt
     5881 tcggcgcgga aatgccagcg cgcgaaccgc tcccactcgt ggaagctcgc cgcgagaggc
     5941 gcccacgtcg tgaatcggtc gcccccggat tgggtggtga agatgttgcg gcagaagttg
     6001 ccgcgccaca cgctccccgc ctggaccggc tgcccgaaga gcgccagcgg caacgccact
     6061 tccagggtgt aggcatcggc cgaccgggaa gcggctgcct gccattccag cgtgccatca
     6121 gagaggccgg ccgcccgtcg ggcacccgcc gtgttgacga tgaactggtg aaacccggcc
     6181 accccctcgg ggcggacgaa gatctccacg ccgtcctcgg cccacagctc gccgccatcc
     6241 ttgccggtgg gctctatcac cgcgatatcc ggctcctcgc agtggatccc gatatagatc
     6301 acccgcccgt cccaggccgc gcgcacttcc gtctgtttcg ccgcggtgta gccgccgccc
     6361 agccggaaga agcccgtggc gactggcact ccctgccacc ccgggtcgcc ctcgatcttc
     6421 ccgtcgagga gcggcgccac ggtcacccgg tagagcgggt attcgagcgt actttctgcc
     6481 gccattgcgg gctccgaaac cgccagaacc acgagcgcga gaatgactct tccgaccaag
     6541 acgcccattc cacgtctcca ccccgcgata gggcttcctg gccctccccc gcaggccacg
     6601 cgatagattg ttgcaaccac catgggcctc cttgatgccc gccggtcacc caccgccggg
     6661 aacgcggggc acccacccgg gccaggccgc gtcacccact cgggggccgg tcacggaacc
     6721 tggggcactg cggaccgcac cgggccggcc ccggaaccag ggcaagcact aaaggttcgc
     6781 ccctacatca cggccggggc cgatcatccc gttcgggcta tcacagactc gccattccat
     6841 gccccagagt cgacacccga gggtttacca ttcaccctcg gccttttgcg accaatcgct
     6901 cccagccggc gagccggtca cccgcagtgc ggccttcccc gtcacgccgg tggcgacatc
     6961 ctggaccacc acgctccagg ttccgggcgc gtcgttgaac gccagtggca
//
LOCUS       DAOSCV010000088         6978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_6963_length_6978_cov_3.006211, whole genome shotgun sequence.
ACCESSION   DAOSCV010000088 DAOSCV010000000
VERSION     DAOSCV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6963_length_6978_cov_3.006211"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /gene="grpE"
                     /locus_tag="PLY56_03440"
     CDS             <1..679
                     /gene="grpE"
                     /locus_tag="PLY56_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ20564.1"
                     /translation="YQISTRDFHRRHEEFTELRRRLAGEVLPPLEAEDDEAARHCHRA
                     LSELLAQLDRRLEDAKEVVLHPGETPGGGGELERRIERLETRAHRLKDGGDACLMELA
                     EPLVQEHLRPLVEMIIRDFLRPLLEEATPVEGETARSARATLLGEVVTLARRVLLESI
                     QLEVIDPKAGSKLEEEAHDEIEVLAPEGDASPNTVAEVVAPGYRWAGEVIQRARVRSY
                     REGGGRA"
     gene            680..>975
                     /locus_tag="PLY56_03445"
     CDS             680..>975
                     /locus_tag="PLY56_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20565.1"
                     /translation="MAGNRSALLSESELRELRALLYATRSKSIDTLQRQVSQIGIRLA
                     DLLVKVAAGLEAGLQETRGHLKVAVETMMEQAEELEQIKAWIEANVGGTVVRYEA"
     assembly_gap    976..985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <986..1861
                     /locus_tag="PLY56_03450"
     CDS             <986..1861
                     /locus_tag="PLY56_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20566.1"
                     /translation="PASLASSPLGGKDAAEVARVAEKLSETETRLAALESEVADVSHQ
                     LRTARDRVETPDRPAGDEALLERLEEHISLLALAGARRVARDRQACIRALERVAEAVE
                     QRFIPRAVAFRRQLSGDVTSYDGCRALEERAREACEELRREARRLGEVAATEAMRLSA
                     APDSGSERWESVRELLALGGVPDGEDDLDFEIRRVLSEWFLPLIDLTVSYRETGAPEG
                     EAAFQALLCDLMEAGWQADLEAIVPARGAAFDPVIHEAVGPAPTGDARVARVESPGYR
                     IGGGIMRLARVHLEQ"
     gene            1880..3874
                     /locus_tag="PLY56_03455"
     CDS             1880..3874
                     /locus_tag="PLY56_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20567.1"
                     /translation="MADGRSESAVEEFLPAPYPAPLFVAWEQHLDFGEVRRPARKRLT
                     FKVSNIGGDGNLDVTLRPNIPDQQWFQVRPTHLVIPAGEDRIVDVEIDTSLESCPLGR
                     HDLDIQVEAPGSMEPEIPRIRVSVEVLREIVLVCDPGEIDLGEVPLGESRTVTVSIQR
                     SDEREVCLNERLIQLVDETGKPRPAWADIELGAPGRKQEVTLTFHTANRAMREYRCEL
                     RLCDTVPEVNQVAVPIHVSVSPPPQLQVEWVIPPMPNFSGDFQDAQLAVGNSGGGVLR
                     GRIAGDQHWLRVLNGEFSVAAGQMHTATVRIGDARLRVGTYHGHLEIFSNDGGRSRVL
                     RVPIELEVVDRSRCPVEIYFEPDLGALHRRQTAERALTITNLMAQPFEGKLISGSPEW
                     LRVKGGMEEVRLGPGESRQLLLEVDPRRLPAQDGPTRHKAALQLVDSTGKERWKKTAT
                     LELVPAPSSPLVRLLLAFWALVLLAGLAWGGKQVYDGPVQRILSARTRAATVATPAPA
                     PAAPPAQQQAATARPAPAQPAPESQSAPEATEKPRRVAPDSARARSLYAEGTAHFQRG
                     EWRLAADLFDQAAAVDPAPAGSLLMVGRSRYHLGEWDAAAKALEEATRLKPDSAAAHL
                     WLGRVYLEQGNREAASREFEAALKADPMNRQARDELQKLR"
     gene            3852..5117
                     /locus_tag="PLY56_03460"
     CDS             3852..5117
                     /locus_tag="PLY56_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HOJ20568.1"
                     /translation="MSFRSFGNRGWRRLLLATLVGAASLGACPRVWAEAGEGVETVQE
                     QAGQIAQKIAPYKGKFVVFVLDVSGSMTNQGRLQASNEALATIVRNGTRVGDRVAIFS
                     FDVSPELLLKETVIQKESDKKIVIDRIPTVSSNTVGSNIRWAHHEALKLLEPSECPYQ
                     VCVLVSDNLHDPPGETDRFYPDYLNYYIPGTMEAPSTPQSNDYARLREKFRGGTRLTF
                     GIGVNISETGAVQELPPLKKTPAWVWLVAAAAVVALAGAGWVGASAWRRAAEARQIAN
                     SPMNVVIRNVTPDGTASPFSAETHLVLQPNESFTIGGAPFGIGQVVSLPGAMMKEPVA
                     RVHRRGLSFSLEALPGQETVAVYVDGIEAVPGGDAIPIRLGQTISVRYRVMAGIEKSV
                     DLLFADPRAAEEQAATEEVVFDESHQSPY"
     gene            5149..>6978
                     /locus_tag="PLY56_03465"
     CDS             5149..>6978
                     /locus_tag="PLY56_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HOJ20569.1"
                     /translation="MAVELDAVGVSRSIIIGCGGTGHRIILETRKRLVDKYGSLSKIP
                     IVQFLQLDTTPLKEEVRYAKEVNLTPNEYVFMQVQNIAKYRRGLENEPHLYPHLASWV
                     PRSILTTNVDQGAGQVRARGRFAFFENYPTIRERLYAAMNRVTSDQAYREATSNGIRV
                     SEGGISVYILTSLMGGTGSGIFLDLAYLAKNELSQAAGAKTIDTIGILLLPPVGGDLV
                     GVYSANAYASLMELNHFNNPATEFVAQYQPGMPELRDPDDPFTFCYLMDSRNEEGDVI
                     GEDKLIQMIGHYLFLDLTHEFASKKRENRDNHKQWLRRDEFDCPTKYLSFGLSALCFP
                     KDQVLHVCANRLAKELVSGWMDPASVFDDRQLQEEASNRMAQANWDCDRVRAGLAVEG
                     QSQHGRSATDAVGEMVRRAEATAKQAMNESAIRALERLIQQDLPPWISAGDEANPDVR
                     QRRGLGIYPEEIRKGLERRIEHMSAWLVELVTELVNDPKYRHEGCRRMLACLERIATQ
                     SRDRLIRDSTELKARMDALNGQIQKMLSQSRGEIRKAMESAANGSRRKAGRGATSNRG
                     TAEAAISSFCRAVAQHAQASVQAFVCDAAHRFYTEMLACLAERN"
BASE COUNT         1208 a         2145 c         2542 g         1073 t
ORIGIN      
        1 gtaccagatc tccacgcgtg attttcaccg tcggcacgag gagttcacag agctccgccg
       61 ccggcttgcc ggggaggtgc tgccgcctct ggaggccgag gatgatgaag cggcgcggca
      121 ttgccaccgg gcgctttcgg aactgctggc tcagcttgat aggcgcctcg aggatgcgaa
      181 agaagtggtt ctccacccgg gcgaaacccc gggtggaggc ggagagctgg agcggcgcat
      241 cgagcggctg gagacgcgcg cgcaccggct gaaggatggt ggtgacgcct gtctgatgga
      301 gctggccgag ccgctggtgc aggagcacct gcgtccctta gtcgagatga tcatccgcga
      361 ttttctccgg ccgctgctgg aggaggcgac accggtggaa ggagagaccg ccaggagtgc
      421 ccgcgccacg ttgctgggcg aagtggtaac gctcgcgcgt agagtcctgt tggagagcat
      481 ccagttggag gtgatcgacc caaaggcagg cagcaagctg gaggaggagg cgcacgacga
      541 gatagaggta ctcgccccgg agggagacgc gagcccgaac acggtggcgg aggtggtcgc
      601 gccgggctac cgctgggcgg gcgaggtgat ccaaagggcg cgcgtgcgct cctatcggga
      661 gggagggggc agggcctgaa tggcgggcaa ccggagcgcg cttctctcgg aatcagagct
      721 ccgcgagctg cgcgcgctgc tctacgcaac ccggtcgaag agcatcgaca ccttgcagcg
      781 gcaggtgagc cagatcggga tccgcctggc ggatctcctc gtcaaggtgg ctgccgggct
      841 cgaggccggc cttcaggaga cgcgcgggca cctgaaggtg gcggtcgaga cgatgatgga
      901 gcaggcggag gagctggagc agatcaaggc gtggatcgag gcgaatgtcg gcggaactgt
      961 cgtccgctac gaggcnnnnn nnnnnccggc ctccctcgcc agctcccccc taggtggaaa
     1021 agacgctgcc gaggtcgccc gcgtggcgga gaagctctcc gagacggaga cgcgcctggc
     1081 cgccctcgaa agcgaggtgg cggatgtgag ccaccagctg cggaccgcgc gcgaccgcgt
     1141 cgagacgccc gaccggcctg cgggggacga ggcgctgttg gagcgcctgg aggagcacat
     1201 ctctcttctc gccctggcgg gggctcgccg ggtggcgcgc gaccgccagg cgtgcatccg
     1261 ggcgctcgag cgtgtggccg aagcggtcga gcagcggttc atcccgcgcg ccgtggcctt
     1321 ccgccggcag ctgagcggcg atgtgacgag ctatgacggc tgccgggctc tggaagagcg
     1381 ggcgcgcgag gcgtgcgagg agctgcgccg cgaggcacgc cgcctgggag aggtggcggc
     1441 gacggaagcc atgcggttga gcgccgcgcc cgactcgggg tcggagcgat gggagagcgt
     1501 tcgcgagctc ctggcgctgg gcggagtgcc cgatggcgag gacgacctcg attttgagat
     1561 ccggcgcgtg cttagcgagt ggttcctccc gctcatcgat ctcaccgtct cctatcggga
     1621 gacgggggct ccagagggag aggcagcgtt tcaggcgctc ctgtgcgatc tgatggaggc
     1681 gggctggcag gcggatctcg aggcgattgt gcccgcgcgg ggggcagcgt tcgacccggt
     1741 gatccacgag gcggtgggtc cggctcccac tggggacgcg cgcgtggcgc gggtggagtc
     1801 gcccggttac cgcatcggcg gcgggatcat gcgcttggcg agagtgcatc tcgaacaata
     1861 gaagctgatg ggagcagtga tggcggacgg tcgttcggaa agcgcggtgg aggagttcct
     1921 gccggcgccc tatccggcgc ctctcttcgt ggcgtgggag cagcacctcg atttcgggga
     1981 agttcgccgg ccagcgagga agcggttgac attcaaggtc tccaacatcg gcggggatgg
     2041 taacctggat gtgacgctcc gaccgaacat cccggaccag cagtggttcc aggttcgccc
     2101 gactcatctc gtgattccgg cgggcgagga tcgcatcgtt gacgtggaga tcgatacgag
     2161 cctggaatcg tgccccctcg gccggcacga cctcgacatc caggtagagg cgcccggatc
     2221 gatggagccg gagatcccgc gcattcgggt gtcggtggag gtgctccggg agattgtgct
     2281 ggtgtgcgac ccgggcgaga tcgacctggg cgaagtgccc ctgggcgaga gccgcaccgt
     2341 gacggtgagc atccagcgca gtgatgaacg cgaggtttgc ctgaacgagc gcctgatcca
     2401 gctggtggat gaaaccggca agccccggcc ggcgtgggcc gacattgaat tgggagcgcc
     2461 gggccggaag caggaggtca ccctcacttt ccacacggcc aaccgggcga tgcgcgagta
     2521 ccgctgtgaa ctgcgcctgt gtgataccgt ccctgaggtg aaccaggtgg cggtgcccat
     2581 ccacgtttcc gtcagcccgc cgccgcagct ccaggtggag tgggtgatcc cgccgatgcc
     2641 gaacttctcc ggcgacttcc aggatgcgca actggcggtg ggcaactccg ggggtggcgt
     2701 gctgcgcggc cgcatcgccg gcgaccagca ctggttgcgc gtgctgaatg gcgagttcag
     2761 cgtggcggcc ggccagatgc acacggcgac ggtccgaatc ggtgacgccc ggttgcgggt
     2821 tggcacgtat cacggtcatc ttgaaatctt ctcgaatgac ggcgggcgta gccgcgtgct
     2881 gcgcgtgcca atcgagctgg aggtggtgga ccggagccgc tgccccgtgg agatctactt
     2941 cgagccggat ctgggcgcgc ttcaccgccg ccagacggcg gagcgcgcgc tcacgatcac
     3001 taacctgatg gcccagccgt tcgaggggaa gttgatctcg ggaagccccg agtggctccg
     3061 ggtgaagggg ggcatggagg aggtgcgcct tggcccgggc gagtcgaggc aactcctcct
     3121 ggaggtcgac ccgcggcgtc tccccgcgca ggacggcccc accaggcaca aggcggcgtt
     3181 gcagctggtc gattccacgg gcaaggaacg ctggaaaaag accgcgactt tggagctcgt
     3241 gcccgcaccg tcctcgccgc tcgtgcggtt gcttctggcc ttctgggcgt tggtgctcct
     3301 cgcgggcctg gcatggggcg gaaagcaggt gtatgacggc ccggtgcaaa ggatcctctc
     3361 cgcgcgcacc cgggccgcga cggtggccac gccggcgccc gcaccggcgg caccgccggc
     3421 tcagcagcag gcggccacgg ccagaccggc cccggctcag ccggctcctg agtcgcagag
     3481 cgcgcctgag gccacggaga aaccacgcag ggtagcgccg gattcggccc gggcgcgctc
     3541 tctctacgcg gaggggaccg cgcacttcca gcggggagag tggcgcctgg ctgccgacct
     3601 ctttgaccag gctgcggcgg tggatcccgc gcccgctggg tcgcttttga tggtgggccg
     3661 cagccgctac cacctcggcg agtgggatgc cgcggcgaag gcgctggagg aggcgacgcg
     3721 gctcaagccc gattcggcgg cggcccactt gtggctgggc cgggtctatc tggagcaggg
     3781 caaccgggaa gcggcctcgc gtgagtttga ggccgcgctg aaggcagatc cgatgaatag
     3841 gcaggcacgc gatgagcttc agaagcttcg gtaaccgtgg gtggaggcgg ctccttcttg
     3901 ccacgcttgt tggcgcggcc agcctgggcg cgtgcccgag ggtttgggca gaggcaggcg
     3961 agggcgtcga gactgtgcag gagcaggcgg ggcagatcgc gcagaagatc gcgccgtaca
     4021 aggggaagtt tgtcgtcttc gtcctggacg tctctgggag catgacgaac caggggcggc
     4081 tgcaggcgag caacgaggcg ctggccacga tcgtgcgcaa tggcacgcgg gtgggcgacc
     4141 gcgtcgcgat cttctccttc gacgtcagcc cggagctgct gcttaaggag acggtcatcc
     4201 agaaggagag cgacaagaag atcgtgatcg accggatccc gacggtgagc agcaacacgg
     4261 tgggctcgaa catccggtgg gcgcaccacg aggcgctcaa gctgctggag ccgtcggagt
     4321 gtccttacca ggtgtgcgtc ctggtgagcg acaacctgca cgacccgccg ggtgaaacgg
     4381 accgcttcta tccggattac ctgaactact acattcccgg cacgatggag gcgccctcca
     4441 ccccgcagag taacgactac gctcgcctgc gcgagaagtt tcgcggcggc acccggctca
     4501 ccttcgggat tggcgtcaac atctccgaga cgggcgccgt gcaggagctg ccgccactga
     4561 agaagactcc ggcctgggtg tggctggtgg ccgcggcggc ggttgtcgcg ctggctggcg
     4621 ccggctgggt gggagcaagc gcgtggcgac gcgccgcaga ggcacgccag atcgccaaca
     4681 gtccgatgaa cgttgtgatt cgcaacgtca ccccggatgg cacggccagc cccttcagcg
     4741 cggagacgca tctggtgctg cagccgaacg agtcgttcac catcggaggc gcgcctttcg
     4801 gtatcgggca ggtggtttcc ctccccggcg ccatgatgaa ggaaccggtg gcgcgagtcc
     4861 accggcgcgg ccttagcttc agcctcgagg cgctgcccgg ccaggagacg gtggcggttt
     4921 acgtggacgg catcgaggcc gtgcctggcg gagatgcgat ccctatccgc ttggggcaga
     4981 cgatcagcgt gcgctatcgg gtgatggctg ggatcgagaa gagcgtggat cttctcttcg
     5041 cagacccgcg ggcggccgaa gagcaggcgg caaccgagga agtagtcttc gacgagagtc
     5101 accagtctcc ctattgaggg ggagcggcat agcagggggg agcagacgat ggccgtggag
     5161 ttggatgccg taggcgtatc ccgaagcatc atcattggct gcgggggcac cggacacagg
     5221 atcatcctgg agacgcgcaa gcgcctggtg gacaagtacg ggtcgctgag caagatcccg
     5281 atagtgcagt tcctacagct cgatacgaca ccactcaagg aggaagtgcg ctacgcgaag
     5341 gaggtcaacc tgacccccaa cgagtacgtc ttcatgcagg tgcagaacat cgccaagtac
     5401 cggcgtggcc ttgagaatga gccccacctc tacccgcatc tggcgtcctg ggtgccgcgc
     5461 tcgatcctca cgacgaacgt cgaccagggc gcgggccagg tgcgtgcgcg ggggcgcttc
     5521 gccttctttg aaaactaccc tacgatccgc gagcgcctct acgcggcgat gaaccgcgtc
     5581 accagcgacc aggcgtatcg cgaggctacg tcgaacggca tccgggtaag cgaaggcggg
     5641 atctcggtct acatcctcac ctcgctgatg gggggcacgg ggagcggcat cttcctggac
     5701 ctggcctacc tggccaagaa tgagttgagc caggccgcgg gcgcgaagac gatcgacacg
     5761 attggcatcc tgttgctgcc cccggtgggc ggcgacctgg tgggcgtcta ctctgcgaac
     5821 gcctatgcct cgctgatgga gctcaaccac ttcaacaacc ccgccaccga gttcgtggcc
     5881 caataccagc cgggcatgcc cgagttgcgc gatccggacg accccttcac cttctgctac
     5941 ctgatggact cgcgcaacga agagggagac gtgatcggag aggacaagct gatccagatg
     6001 atcggccact acctcttcct cgatctcacg cacgagttcg cgagcaagaa gcgcgagaac
     6061 cgcgacaacc acaagcagtg gctgcgccgc gatgagttcg actgccccac caagtacctt
     6121 agcttcggcc tctcggcgct ctgcttcccc aaggaccagg tgcttcacgt ctgcgccaat
     6181 cggctggcga aggagctcgt ctcggggtgg atggaccccg cctcggtctt cgatgaccgc
     6241 cagctgcagg aggaggccag caaccgcatg gcccaggcca actgggactg cgatcgcgtg
     6301 cgggccggac tggcggtaga gggccagagc cagcatggga ggagcgcgac cgatgccgta
     6361 ggcgagatgg tgcgccgcgc tgaagccacc gccaaacagg cgatgaacga gagcgcgatt
     6421 cgcgccctgg agaggctgat ccagcaggat ctccccccct ggatctccgc cggcgacgag
     6481 gccaatccgg acgtgcgcca gcgcaggggg ctgggcatct acccggaaga gatccgcaag
     6541 gggcttgaac ggcgcatcga gcacatgagc gcgtggctgg tggagctggt gacggagctg
     6601 gtgaacgacc cgaagtaccg ccacgaaggc tgccgccgca tgctggcctg cctggagcgg
     6661 attgcgaccc aatctcgcga ccggttgatc cgcgacagca ccgagctgaa ggcgcggatg
     6721 gacgcgctga atggtcagat ccagaagatg ctgagccagt cgcgtggcga gatccggaag
     6781 gcgatggaat ccgccgccaa cggcagccgg cgaaaagccg gccgcggcgc gacctccaat
     6841 cgcggcacgg ccgaggcggc gatctccagc ttctgccggg cagtggctca gcacgcgcag
     6901 gccagcgtgc aggcgttcgt ctgcgacgct gcccaccgct tctacaccga gatgctcgcc
     6961 tgcctggccg agcgtaac
//
LOCUS       DAOSCV010000089         6873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7086_length_6873_cov_3.516720, whole genome shotgun sequence.
ACCESSION   DAOSCV010000089 DAOSCV010000000
VERSION     DAOSCV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_6873_cov_3.516720"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLY56_03470"
     CDS             <1..305
                     /locus_tag="PLY56_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20570.1"
                     /translation="RRISRWEDIQEYYEQRRRGLTDPPRFTWPNYNFQRMGQHLESLG
                     LVRLGKIGSATLRAIYARDMVDTILATLIAHPEEWLSEPFLSWWYDAVGRGEKRES"
     gene            334..1371
                     /locus_tag="PLY56_03475"
     CDS             334..1371
                     /locus_tag="PLY56_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ20571.1"
                     /translation="MKIWNSNPAWPWPCDEEIAKYLDVPDYYLAGYEEVRGFLRGLHK
                     GRIESAGKSAGGRDLTVVYCGPDAPERTLCVTGGTHGTEVEGVAAVVNTIRVLDCGSD
                     LRGQAWPALTEVARRVGFYLMPFFNPDAAARAVVKSYVGMPFEVVNHLHVGFWKNGRT
                     LDRKRVFGPWTPESDLTGKLDRVGYLGARYNDAGADINRPCSRTEATAVETRQMLEYL
                     RQRRVDCYLDMHSHSTDPWLGICPPEHTSGNHGDLVKLQQLTREKTVQAGGPDLKLAV
                     KGTRTWFNSHFFPANLGIYALTYEEKAGFKGNFPEETPPEEIWLANVYNGMRTILGLA
                     QALLEVPSPWS"
     gene            complement(1469..2239)
                     /locus_tag="PLY56_03480"
     CDS             complement(1469..2239)
                     /locus_tag="PLY56_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ20572.1"
                     /translation="MRDLHCWEQLRPEEFYAEQSRAPVAYWACGPMEDHGLQNAMGVD
                     PGKAYEICRRAARLTGGIVFPMVPFAPAGNPPLSREAMRSGKHPLYPPSLFVSVELCE
                     QIYTELFENLAELGFRLCVAFGGHGPAALLLRRLAERLGGQLGGMKIHTCGSTTYLAD
                     LLPGLDPEDPSPGAHGGLWETAMNAALNPEFVDLTRVRHIDSSPIPSQLKGREERQWA
                     AIERATPEIGERLLDTAARRLASEVEALLAGIEDAATP"
     gene            complement(2425..5028)
                     /locus_tag="PLY56_03485"
     CDS             complement(2425..5028)
                     /locus_tag="PLY56_03485"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ20573.1"
                     /translation="MHVALIGVLLGLLAAPGFAAGFPVLEAGQQAVIVYEAEAETESA
                     KAYQDLASYLKRSTGKEFKTVAEGDYRPGDGLPIYVGRCRATEEALRGDLAGLDRDAY
                     IVAVEPTRVMLVGSRPWSTYWAVCQFLEDYVGVRWLIPGPLGEDVPQHSTLAVPVGKK
                     SYTPALTSRLWSGAHHAGDWNLRQRIRSRDQFHHNLIRIFTPEKYWDTHPEYFPLHGN
                     QRYRPGKDDHSWQPCMGIEGTVQAAADAAREAFRKDPTLESFSFGMNDGQGWCECPAC
                     KAIDRPLPEWHGFSGDKSVLYYTWLNKVAENLEKDYPDKKLGCLAYSSVILPPPFKLH
                     RNIVPYFTSNRADYFDRRFRQRDQELLARWSRCATQMGIYDYAYGVGFAIPRIYNHLF
                     QDSVKFAYAHNVRGFYAEVYPNWGLDGPKLYAMARIVWDPNVDLDALMRDWNERMFRE
                     AAEPMRRYFALAEETWRKQRGAGAWAYRLAADPAQFRIFSPRVMERMTAYLDEAERLA
                     VDETVRKRIQFFRKTWNLTRLLCGNYWAGERVQRLIDRKAPVERVAAAMREMAGQLAT
                     IDVDRWMKENHISDDPIAFFPPLSGWFEPLKAGSTTNAVRYFASVLINDAMAEARRSG
                     TPDAAALRDAIARRVDEVFGDGGSESYREAVARIRDMSLKVGTVAKLDAPVKVDGLLD
                     EAVWQKADVLTDFLKWGDTSRAEHVTKVRLARQGDQLYVGLECFQDTSALVCQAAPRD
                     GSAWKDDSVEIFINKGMDAAPHAQFIINAAGAFFDQYDRDGRQSYAERLAVDFDCDWA
                     AKVFPDKWTAEVRIPLRALGIDPDRQPLVRMNFVRNVQLREGKGSAISAWFSSVRAHA
                     DPLSRGWIVLQ"
     gene            complement(5110..6390)
                     /locus_tag="PLY56_03490"
     CDS             complement(5110..6390)
                     /locus_tag="PLY56_03490"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ20574.1"
                     /translation="MSEKLAIYGGPKSIQPGEIKPWPPVDETDEQLVLDALHSTQQAR
                     GPHNLAFEKEFAEWNGNRYAILTNSGTAALHMGLVGCGVGAGDHVLVTAYSWSSSATC
                     ILHHNAIPIFVDIDFDTINMDIDKIEEAITPRTKAIVAVNLHGLCLNYDKLMAVARKH
                     NLKVIEDCCQAHGAQFRGRNCGTFGDAAAFSLNQNKCICSGEGGVFVTDNEEVYRAAC
                     QLWSFGETRMPEESRDYHAYALGWMYRTNELTAAYARAQLRKYPRHFETLRQNAAVLN
                     RILPGTRALILPVEPDGHLHNWYNYNLRIDFDAAGWTGDRRRFRAAVNAALNAEGVPT
                     SIWQQFILPEMTVFQAKNAYGGGFPWSIPGADEGVDYNPERFPVARRHCESYIALVMA
                     LRAPNGPDMAERIALAIRKVFDQIDQIDPDRILQ"
     gene            complement(6393..>6873)
                     /locus_tag="PLY56_03495"
     CDS             complement(6393..>6873)
                     /locus_tag="PLY56_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20575.1"
                     /translation="HLVPRQELLVDATRVRGAAPAEVVGAVPIRVPAGGSAQARVKVP
                     WRWALPNVELEPNEPPKGITVGHAMALAGDLAFAIKADATAVKPGDADNLIIEAFARQ
                     RDGRRSSLGFLPAIPIEVVPRTGHGVSMRRSPGRHTARGKRRPAVPAPYGMRHHERV"
BASE COUNT         1163 a         2337 c         2220 g         1153 t
ORIGIN      
        1 accgccggat cagccgctgg gaggacatcc aggagtacta cgagcagagg cgcaggggac
       61 tcaccgatcc cccccggttc acctggccca actacaactt ccagcggatg ggccagcacc
      121 tggaaagcct ggggctggtc cgcctcggca aaatcggctc ggccaccctg cgcgcgatct
      181 atgcccgcga catggtcgat acgatcctgg cgacgctcat cgcccaccca gaggagtggc
      241 tgagcgagcc gttcctctcc tggtggtatg acgcggtcgg gcggggcgag aagcgcgagt
      301 cctagccatc agagacaccg attgggaaga tacatgaaga tttggaacag caaccccgcg
      361 tggccctggc cgtgcgacga ggagatcgcg aaatacctgg atgtgccgga ttactatctc
      421 gccgggtatg aggaggtgcg cggcttcctg agagggctac acaaaggccg catcgagagc
      481 gccggcaaga gcgcgggcgg gcgcgaccta accgtggtct attgcggccc cgatgcgccc
      541 gagcgcaccc tatgcgtcac cggcggcacc cacggcaccg aggtggaggg cgtcgccgcc
      601 gtcgtcaaca cgatccgggt tctcgattgt ggctcggacc tgcgcggcca agcctggccg
      661 gccctcactg aggtggcgcg tcgcgtcggc ttctacctga tgcccttctt caacccggac
      721 gcggccgcgc gcgccgtcgt caagagctac gtcggcatgc cgtttgaggt cgtcaaccac
      781 ctgcacgtcg gcttctggaa gaatgggagg acgctcgacc gcaagcgcgt cttcggcccg
      841 tggaccccgg aatcagacct caccggaaag ctggatcgcg tcggctattt gggggcgcgc
      901 tataacgacg cgggcgctga catcaaccgc ccctgctccc gaaccgaggc cacggcggtc
      961 gaaacccggc agatgctcga gtatctccgc cagcgccggg tcgactgcta cctggacatg
     1021 cactcgcaca gcactgaccc gtggctgggc atctgcccgc ccgagcacac ctccgggaac
     1081 cacggcgatc tcgtgaagct gcagcagctg acgcgcgaaa agacggtgca ggccggcggc
     1141 cccgacctga aactggcggt gaagggcacg cgaacctggt tcaacagcca cttcttccct
     1201 gccaacctgg ggatctacgc gctcacgtac gaggagaaag ccgggttcaa aggcaacttc
     1261 cccgaagaga cgccgcccga ggagatctgg ctggccaacg tgtacaacgg catgcgcacg
     1321 attctcggtc tggcgcaggc gctcctggag gtcccgtcgc cctggagctg atggccggct
     1381 cgctcccccc acggcagagc gccccggggg ggagcgagcg caaccaaaca tcccttgcca
     1441 ctactggcgc cggcaccggt cgccgtcctc aaggggtcgc cgcgtcctcg atacctgcca
     1501 ggagggcttc cacctcgctg gccaagcgac gggcggccgt atccagcagg cgctcgccaa
     1561 tttccggggt cgcccgttcg atagcggccc attgtctctc ctcccggccc ttgagctgcg
     1621 agggaatcgg actggagtcg atgtggcgca cccgcgtgag gtccacgaac tccgggttga
     1681 gcgcggcatt catcgccgtc tcccagagac caccgtgcgc gccgggggat ggatcctctg
     1741 ggtcgagccc gggcaaaagg tcagcaagat aggtggtgct gccgcaggta tggatcttca
     1801 tcccgcccag ctgcccgccc aggcgctcgg ccaggcgccg gagcagcaac gcggctggcc
     1861 cgtgcccgcc aaacgccacg cagaggcgga agcccagctc cgccaggttt tcaaacagct
     1921 cggtgtagat ctgctcgcac agctccacgc tgacgaagag gctgggtgga tacagcgggt
     1981 gcttccccga gcgcatcgcc tcgcgtgaca gcggcgggtt gccggccggc gcgaacggca
     2041 ccatggggaa gacgatgccc cccgtcaggc gcgcggcccg acgacagatc tcatacgcct
     2101 ttcccgggtc gacgcccatg gcattctgca ggccgtggtc ttccatcggg ccgcaggccc
     2161 agtaggcaac cggcgcccgg ctttgctcgg cgtagaactc ctccggccga agctgctccc
     2221 aacaatgcag gtcgcgcatc ggatctctcc cagcccgggg cacgcggccg tgccccgggc
     2281 ccagcaatcg gaagcggcca cctggccacc acacgggaag cttcctggcg atacccccca
     2341 ggggatcggc gtcaggtacc ggccggcgag accagccaaa gctcccggct ggttctctca
     2401 cccaacggtc ggccagcgtg ttcgctactg gagcacgatc cagccccggc tcaatggatc
     2461 ggcatgggcg cgcacggacg agaaccacgc ggagatcgcc gaacccttcc cctcgcgtag
     2521 ctgcacattg cgcacgaagt tcatgcgcac cagcggctgg cgatccgggt cgatccccag
     2581 ggctcgcagc gggatccgga cttccgccgt ccacttatcg gggaagacct tcgcggccca
     2641 atcgcagtcg aagtcaactg ccaggcgctc ggcgtagctc tgccgaccgt cgcgatcata
     2701 ttggtcgaag aacgcgccgg cggcgttgat gatgaactgg gcgtgcggcg cggcatccat
     2761 ccccttgttg ataaagatct cgacggaatc gtccttccac gccgagccat cgcgcggcgc
     2821 cgcctggcag accagcgcgg aggtgtcctg gaagcactcc aggccgacgt agagctgatc
     2881 accctggcgc gccaggcgca ccttcgtcac gtgctccgcc cggctcgtat cgccccactt
     2941 gaggaagtcg gtgagcacgt ctgccttctg ccacaccgcc tcgtcaagca gcccgtctac
     3001 cttcacgggg gcatccagct tcgcgacggt tcccactttc agcgacatgt cgcggatacg
     3061 ggccactgcc tcccggtagc tttccgagcc accgtcgcca aacacctcat ccacgcgccg
     3121 ggcgatcgcg tcgcgaagcg cagccgcgtc tggggtgccc gaccggcggg cctccgccat
     3181 cgcgtcgttg atcaggacgc tcgcgaagta gcggacggcg ttcgtggtcg atcccgcctt
     3241 cagcggctcg aaccacccgg agaggggcgg gaagaaggcg atcgggtcat cgctgatgtg
     3301 gttctccttc atccaccggt ccacgtcgat cgtcgccagc tggccggcca tctcgcgcat
     3361 cgccgccgcg acgcgctcga cgggcgcctt ccggtcgatc agccgctgca cccgctcgcc
     3421 ggcccagtag ttgccgcaca ggaggcgcgt caggttccac gtcttccgga agaactggat
     3481 ccgctttcgg acggtctcat ccaccgccag gcgctcggcc tcgtccagat aggcggtcat
     3541 ccgctccata acgcgcggcg agaagatccg gaattgcgcg gggtcagccg ccaggcggta
     3601 ggcccaggcg cccgcgcccc tctgcttgcg ccaggtctcc tcggcgagag cgaagtagcg
     3661 gcgcatcggc tccgcggcct cgcggaacat ccgctcgttc cagtctctca tcagggcatc
     3721 cagatcgacg ttcggatccc agacgattcg cgccatcgcg tagagctttg gcccatccag
     3781 gccccagttg gggtagacct ccgcgtagaa gccgcgcacg ttgtgcgcgt aggcgaactt
     3841 caccgagtcc tggaagaggt ggttgtagat gcgcgggatg gcaaagccaa cgccgtaggc
     3901 gtagtcatag atccccatct gcgtggcgca ccgcgaccac cgcgcgagca gctcctggtc
     3961 gcgctgtcgg aaccgccggt cgaagtagtc ggcgcggttg gaggtgaagt aaggcacgat
     4021 gttccgatgc agcttgaagg gcggcggcag gatcacgctg gagtaggcca ggcacccgag
     4081 tttcttatcg ggatagtcct tctccaggtt ctcggccacc ttgttgagcc aggtgtagta
     4141 gagcaccgac ttatcgcccg agaagccgtg ccattcggga agcgggcggt caattgcctt
     4201 acacgcgggg cactcgcacc agccctggcc atcattcatc ccgaaggaga acgattccag
     4261 cgtcgggtct tttcggaacg cctctcgcgc ggcatccgcg gccgcctgga cggtcccctc
     4321 gatccccatg cacggctgcc aggagtgatc gtccttgccg ggccggtagc gctgattgcc
     4381 atgcagcggg aagtactccg gatgcgtgtc ccaatacttc tcgggcgtga agatccggat
     4441 caggttgtgg tggaactggt cacgcgagcg gattcgctgg cgcaggttcc agtcgccggc
     4501 gtggtgcgcg ccgctccaca gccgcgaagt gagcgccggc gtgtagctct tcttccccac
     4561 gggcacggcg agcgtcgaat gctggggcac atcctcgccc agcgggcccg ggatcagcca
     4621 gcgcacgcct acatagtcct ccaggaactg gcacaccgcc cagtaggtcg accagggccg
     4681 cgagcctacg agcatcacgc gcgtgggttc cacagcaaca atgtaggcat cgcgatccag
     4741 gccggccaga tcaccgcgca acgcctcttc cgtcgcacgg cagcggccga cgtagattgg
     4801 caggccgtcg cccgggcggt agtcgccctc ggcaaccgtc ttgaactcct tgccggtcga
     4861 gcgcttcagg tacgaggcaa gatcttgata ggccttggcg ctctccgtct ccgcttccgc
     4921 ctcatagacg atgaccgctt gctggcctgc ctcgagaacc ggaaagccag ccgcaaagcc
     4981 cggggcagcg agcagcccga gcagcactcc gatgagcgcg acatgcatca atcgctcctc
     5041 caatcgcgta atagcaatgc cagggttcga cagcgaagct gccgttccct ggcatgtggt
     5101 atccccccct tattggagga tgcggtccgg gtcgatctga tcgatctggt cgaacacctt
     5161 gcggatggcg agggcgatac gctccgccat gtcggggccg ttgggtgcgc gcaaggccat
     5221 caccagggcg atatagctct cgcagtgccg ccgggccacg gggaagcgct ccgggttgta
     5281 atccacgcct tcatcggcgc cggggatcga ccaggggaag ccgccgccat aggcgttctt
     5341 cgcctggaag acggtcatct ccggcagaat gaactgctgc cagatggagg tgggcacgcc
     5401 ctccgcgttc agggcggcgt tgacggcggc gcggaagcgc cggcggtcgc cggtccagcc
     5461 agccgcgtcg aaatcgatgc gcagattgta gttgtaccag ttgtgcaggt ggccgtctgg
     5521 ctccacgggc aggataaggg cacgcgtgcc tggcaggatc cggttcagca ccgcggcgtt
     5581 ttgtcgcaac gtctcgaagt ggcgcgggta tttgcggagc tgcgcgcgcg cgtaggccgc
     5641 ggtcagctcg ttggtgcggt acatccagcc cagggcgtag gcgtgatagt cgcgcgattc
     5701 ctccggcatg cgcgtctcgc caaagctcca gagctggcag gcggcgcgat agacttcctc
     5761 gttatcggtc acaaagacgc cgccctcgcc actgcagatg cacttgttct ggttcaggct
     5821 gaaggctgcc gcgtcgccga aggttccaca gttgcgcccg cggaactgcg cgccatgcgc
     5881 ctggcagcag tcctcgatca ccttcaggtt gtgcttgcgc gccaccgcca tcagcttatc
     5941 gtagttgagg cagagaccgt gcaggttgac ggcgacaatc gccttcgtcc gtggggtgat
     6001 cgcctcctcg atcttgtcga tatccatgtt gatcgtgtcg aagtcgatat cgacgaagat
     6061 cgggatggcg ttgtggtgaa ggatgcacgt ggcgctgctg ctccaggagt aagcagtgac
     6121 gaggacgtgg tcgcctgcgc cgacgccgca cccgaccagg cccatgtgca gcgccgcggt
     6181 gccgctgttg gtgaggatgg cgtagcggtt gccgttccac tccgcgaact ctttctcgaa
     6241 cgccaggttg tgcggccccc gcgcctgctg cgtcgagtgc agcgcgtcca gcaccaactg
     6301 ctcgtcggtc tcatccaccg gcggccaggg cttgatctcc cctggctgaa tgctcttcgg
     6361 tccgccgtaa atggcaagct tctcactcat ggtcacaccc tttcatgatg cctcatccca
     6421 tagggcgctg gcacggccgg acggcgctta ccacgcgccg tgtgccttcc aggcgagcgc
     6481 ctcatcgaca ctccgtgccc ggttctgggg accacctcaa tcggtatggc cggcagaaag
     6541 ccgagggatg aacgccggcc gtcccgctgc cgggcgaacg cctcgataat caggttgtcg
     6601 gcgtctccgg gcttcaccgc ggtcgcatcc gctttgatgg cgaacgccag gtctcccgcc
     6661 agggccatgg cgtggcccac cgtaattcct ttggggggct cattcggttc tagctcgacg
     6721 ttgggaagcg cccaccgcca gggcaccttc acgcgcgcct gcgccgaccc acctgcggga
     6781 acccggatgg ggacggcgcc gaccacctcg gcgggcgcag ccccacgcac gcgcgtggca
     6841 tcgaccagaa gctcctggcg cggcaccagg tgc
//
LOCUS       DAOSCV010000090         6868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7093_length_6868_cov_4.655365, whole genome shotgun sequence.
ACCESSION   DAOSCV010000090 DAOSCV010000000
VERSION     DAOSCV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7093_length_6868_cov_4.655365"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            578..2437
                     /locus_tag="PLY56_03500"
     CDS             578..2437
                     /locus_tag="PLY56_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ20576.1"
                     /translation="MRALTTGLVSVALVLLALHAPVSAQQPAVPAGAKESVPLLQLAP
                     PSPHESYLPFPALGPVELDEANNGIGVAQEIARKRQVQGRVLWIDAVANLERINSAEK
                     VKALVERIASVGFNTIVLDVKPIVGETLYPSQHAKRLTRWNQATLPSDFDPVAAMAEA
                     CQAAKIGLFASMNTFSEGHRGMSRGPAYERTDWQTVVCEAQLCVVDPKSGVRLLINPQ
                     PNTRAEALDELTVYTRTDVLAPTDDATVVLLDASRKVISVTSGAAFTLAGLSPPSGGA
                     ILVGEGQAGAFLRRIASVGQMLQLQTATRFVPILQSRIPGVPVWVNPHHPEVQQRLLE
                     MITELLTRYPLDGVVFDDRLRYAAINADFSESTRQAFEEWMGRAMTWPDDVLRCHVSF
                     PGMAVRWIPGPHMDAWLVWRALHLRNWLARAAATARTARPTATVGAYVGSWYGDYPAL
                     GANWAADDFQAGFRFLTDTFRPTGFAGLLDWIAPGCYYGMPTIADAIQRGYSPGATVE
                     AAGQLANRAANSQAWVYAGIALEKYDRNPDGLARALQAAAASTQGVMVFDLSHRIDQF
                     WPVFRKAFAKPAQAPHAVPGLLAEVRRLQAERKAAGIVDPPVIIQNGLSGTGL"
     gene            2676..5351
                     /locus_tag="PLY56_03505"
     CDS             2676..5351
                     /locus_tag="PLY56_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ20577.1"
                     /translation="MAQGYVLHVVSHTHWDREWYLPFQRFRMRLVDLIDHLLDLLDRD
                     PEFRCFHLDGQTLLLEDYLEIRPENEERLRRHIREGRLLIGPWYQQNDGFLVSGEATI
                     RSLLIGHRIARHFGGVMKVGYLPNQFGNISQMPQILRGFGIESCIFGRGFLPNGSCME
                     FIWQGADGSRVLANSLVFSYSNLARLPEEPEEALAELRHARDLLAPLAKTRHLLLMNG
                     ADHLEAQETLSAILEALRARLDAEGSPDEVIHSSLPQFVEAVRAEIPCKEGRQDLPVV
                     TGELRTDRDRMVLAGTLSARIDLKQENWRSQTLLEKWAEPFSVWAWLAGSADPHAALR
                     YAWKLLLQNQTQNAICGSSIDEVYDEMMPRFRQVQQIAEEVTERSLGFLVERIHTGPE
                     PPGGPAAALVAFNPLPFRRRECIEAVVEFPLGPPRRMPVAPEVDLAEGGPDVSEVQVV
                     DPAGAIIPVQVLENRLATRRILSGNDLPHLQSVRRMRILFAGEDLPACGYATFHILPA
                     GVQPPPMQTLSPSLHALENAPLRVDIAPNGSLHVIDKDTGREYGGLLVFEDGGDAGDL
                     YHYVQPVQDRVFTTLGSAPRISLVEDGPVRSTYCIEHTLMLPENLKARTAGDGHTMPC
                     RITSYIALCIDSQRVEVTTVVENQACDHRLRVLFPTGAHTDLSRAEGPFDVLKRPAAL
                     PAEWIGGSPCRPQHSFVDVSDGQRGIAILNQGLPEYEVKRDVDRTIALTLLRCVGRLS
                     DGDDAPGIDPTPGAQCLGRHVFRYALMPHAGSWEAARIWEQAHAFNAPIRPVQTDQAD
                     GDLPANASFVEIRPAHLVLSAIKRAEDEDALVLRFYNITNANAAGEVILHFPIRAAYR
                     ANLNEEKAQPLDVNDGRRIRVPVRGKEIVTLLLYR"
     gene            complement(5368..>6868)
                     /locus_tag="PLY56_03510"
     CDS             complement(5368..>6868)
                     /locus_tag="PLY56_03510"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20578.1"
                     /translation="LSLRQLRDDSYTLEPAAPAGVKTERADGWIDLSNGRWGVTAAVR
                     DFWQLYPKGIGVDAEGLKIDLCPDFPEGTYDGCSKLDEIKLYFALMRGKYRVRRGVQK
                     QHDLLLAFHPGQADGDAARHVSQAFQEPLIAVCTPERYCDTLVFGEILPATAGRSPEY
                     EKVCEGVYENYLRHRDATRGYGLLNFGDQFGERKVNWSNGEYDHHHAFLLQFIRTGDR
                     RWYFLGDRAARHAIDVDTCHHGPRAGGVWIHAMGHTGGYFREQYEGSGIPGGGFTPSH
                     TWTEGFCDWYFLSGDPTAAENAALVADYYGGAYLNNYDYSNCRDNGWHLLLTLATYRL
                     TNDPYYLNAARIIVERTLERQTPGGGWHRQMVPGHCYCMPRHRGEANFMMGVLANGLA
                     EYYRETSDQRVAQALLGGAKQVVAELWVEDANGFRYTSCPKMKGYIANNDMTAGMLFY
                     AYRLGGDRQYADIAMRAMKAAFDGGIRSISHLRWTPRLIYHMDRVARGE"
BASE COUNT         1123 a         2347 c         2202 g         1196 t
ORIGIN      
        1 aggccgtccg cacgcgcgga gctcgaagct ccgcgctgct ttccggcaaa gccccttcgg
       61 gccttctcat cccagccacc gccgttttga gacatctggg agtatcccca acccagcccg
      121 gcagggcttc gctcgcaagc agcgcggtga tttatcgccg cgcgccgggt tgcgcaatgc
      181 ctccgaggct tcggattcct tatgtcgaaa tctcagggcg aggctccgtc ttgttatcca
      241 cagatggcac gatgaacaca gataggtgaa cattgcagaa cagaccggct gtgcgagagc
      301 aaacaccgcc tgcgccgatt gtccgtactt ttactcatct gctatccgcg gacgatcgat
      361 acgccatgcc gggctggcgc ggtggaatac ctgagtgaac taaggcgcgc ggctcgccgt
      421 cccccatacc agttacccac tgggcttctg cggcccggcg aacggcagag agggagcgag
      481 gcgccgtccc tgcccaaccg agcctggctc gtgtctggcc tctcttcgtg attccggctg
      541 caccacagtc cccattcgcc atcatagagg aggcagcgtg cgcgcactca caaccggtct
      601 tgtttccgtt gctctggttc ttctggcgct ccatgcgccc gtctcggcgc aacagccggc
      661 cgtgcccgcg ggcgcaaagg agagcgtgcc cctgctgcaa ctggcgccgc catcgccgca
      721 cgagagctat ctgcccttcc ccgccctcgg gccggtggag ctggatgagg cgaataacgg
      781 cattggcgtt gcccaggaga tcgcccggaa gcgccaggtg cagggacgcg tcctctggat
      841 cgatgccgtc gccaacctgg agcggatcaa ctcggctgag aaggtcaagg cgctcgtgga
      901 acggatcgct tccgtcgggt tcaacacgat tgtgctggat gtgaagccga ttgtgggcga
      961 gaccctctac cccagccagc acgcgaagcg cctcacccgg tggaaccagg cgacgctgcc
     1021 gtcggacttc gacccggtcg cggcgatggc cgaggcctgc caggccgcca agatcgggct
     1081 cttcgccagt atgaacacct tcagcgaggg ccaccgcggc atgtcgagag ggccggccta
     1141 tgagcgcacc gactggcaga cggtggtgtg cgaggcgcag ctgtgcgtgg tggatcccaa
     1201 gagcggcgtg cgtctcctta tcaatcccca gccgaacacg cgcgccgagg cactggacga
     1261 gctgacagtc tacacccgta ctgacgtgct ggcgcccacg gacgatgcca ccgtcgtgct
     1321 cctggatgcc tctcgcaaag tgatcagcgt gacgagcggc gctgccttca ccctcgcggg
     1381 tctctctccc ccctcgggag gtgccatcct cgttggggaa ggccaggcgg gcgctttcct
     1441 gcgccggatc gcttcggttg ggcagatgct ccaacttcag acggctaccc ggtttgtgcc
     1501 gatcctgcaa tcgcgcatcc ctggagtgcc ggtgtgggtc aacccgcacc acccggaggt
     1561 ccaacagcgg ctgctggaga tgattaccga gctcctgacg cgctacccgc tcgatggtgt
     1621 ggtgttcgac gaccgcctgc gctacgcggc gatcaacgcc gacttcagcg agtccacccg
     1681 gcaggctttt gaggagtgga tgggccgcgc gatgacatgg ccggacgatg tgctccgctg
     1741 ccacgtgagc tttcccggca tggccgtccg ctggatcccg ggtccccaca tggatgcctg
     1801 gctggtatgg cgcgcgcttc acctgcgtaa ctggctggcg cgggctgcgg ccaccgcgcg
     1861 cactgcccgc cccaccgcta cggtgggcgc ctacgtcggc tcctggtacg gcgattaccc
     1921 cgcgctgggc gccaattggg ccgccgatga tttccaggcg ggattccgct tcctcaccga
     1981 taccttccgc cccaccgggt ttgccggcct gctggattgg atcgcgcccg gctgctatta
     2041 tggcatgcct accatcgccg atgccatcca gcgcggctac tctccaggcg ccaccgtgga
     2101 ggcagccggg cagcttgcca accgcgctgc caacagccag gcgtgggtct atgcgggcat
     2161 cgccctggag aagtatgacc gcaatccgga tggactggcg cgcgccctcc aggcggcagc
     2221 ggcgtccacg cagggcgtga tggtgttcga cctctcgcac cgaatcgacc agttctggcc
     2281 ggtcttccgg aaggcgttcg cgaagccggc tcaggccccg cacgccgttc cggggctgct
     2341 ggccgaggta aggcgactgc aggccgaacg caaagccgct gggatcgtcg atccccccgt
     2401 cattatccag aacggcctct ctggtaccgg cctctagcgc ggcgggcgcc gtggtctccc
     2461 cccgaggcca cgcgcgcccc caccttcccc cgtgccatgc cgagcaaaac gaagcatgcc
     2521 tcgctcccag cgcctgcagg agatcccagt agtctgccgg gcgttggctt cccgcgcatc
     2581 cttccagaag ccggcgcatg tgcagaacgg cgcgcgaatg ggtagaaccc aacggagcga
     2641 ggctgccagc cgcacgacgg cttcaggaga caaggatggc acagggctac gttctgcacg
     2701 tggtgtctca cacgcattgg gatcgcgagt ggtacctgcc gttccagcgc ttccgcatgc
     2761 gcctggtgga cctgatcgac cacctgctcg atctcctcga ccgcgacccg gagttccgct
     2821 gcttccacct ggatgggcag acgcttctcc tggaggatta cctggagatc cgcccggaaa
     2881 acgaggagcg cctgcgccgc catatccgtg aaggccggct cctcatcggg ccgtggtatc
     2941 aacagaacga cgggtttctc gtcagtggcg aggcgaccat ccgcagtctc ctcatcgggc
     3001 atcgcatcgc gcgccacttt ggcggcgtga tgaaggtggg ctatctcccc aaccagttcg
     3061 ggaatatctc gcagatgccc cagatcctgc ggggctttgg gatcgagagc tgcatcttcg
     3121 ggcggggttt tctgcccaac ggctcctgca tggagttcat ctggcagggc gccgacggct
     3181 cccgggttct ggccaactcg ctggtcttct cctacagcaa cctcgcgcgg cttcccgaag
     3241 agccggaaga ggccctcgcc gagcttcgcc acgcccgcga tctcctggcg ccgctcgcga
     3301 agacgcgcca cctgctgctg atgaacggtg ccgaccacct ggaggcgcag gaaaccctca
     3361 gcgcgatcct ggaggcgctc cgtgcccggc tggatgccga gggaagtccc gacgaggtga
     3421 tccattcgag cctgccgcag ttcgtggagg ccgtgcgtgc ggagattccg tgcaaggaag
     3481 gccggcagga tctgccggtg gtgacaggcg agctgagaac cgaccgcgac aggatggtgc
     3541 tggccggcac gctctcagcg cgcatcgatt tgaagcagga gaactggagg agccagaccc
     3601 tcctggagaa gtgggcggag ccgttctcgg tctgggcctg gctggcgggc agcgccgacc
     3661 ctcacgccgc gcttcgctat gcctggaagc tgctgttgca gaaccagact cagaatgcca
     3721 tctgcgggag cagcatcgac gaagtgtatg acgagatgat gcctcgcttc cgacaggtgc
     3781 agcagatcgc ggaggaggtc accgagcgca gccttggctt tctggtcgag aggatccaca
     3841 ccgggcccga accccccggc ggccctgctg ccgcgctggt ggcgttcaac cccctcccct
     3901 tccgccggcg agagtgcatc gaggcggtgg tagagtttcc cctcgggcca ccgcgacgca
     3961 tgccggtcgc gcccgaggtg gacctggccg aggggggccc ggatgtgagc gaggtgcagg
     4021 tggtggatcc cgccggcgcc atcatcccgg tgcaggttct ggagaaccgc ctagccacgc
     4081 gacggatcct ctcgggcaac gatctgccgc atctccagag cgtgcgccgg atgcgcatcc
     4141 tcttcgccgg cgaggatctt cccgcgtgcg gctatgctac cttccatatc ctgccggccg
     4201 gagtgcagcc gccaccgatg cagacgctct ccccctcgct tcacgcgctg gagaacgcac
     4261 ccctgcgcgt cgatatcgcg cccaacggca gcctccacgt catcgacaag gataccgggc
     4321 gcgagtatgg cggcctcctt gtcttcgagg acggtggcga tgccggcgac ctgtatcact
     4381 atgtgcagcc ggtgcaggac cgggtcttca ccacgctggg ttcggcaccc cgaatctcgc
     4441 tcgtggaaga cggcccggtg cgctccacct actgcatcga gcacacgctg atgcttccgg
     4501 aaaacctgaa agcgcgcaca gccggggatg gccacactat gccgtgccgg atcacctcct
     4561 atatcgcgct gtgcatcgat tctcagcgcg tcgaagtgac gacggtggtg gagaaccagg
     4621 cgtgcgatca ccggctgcgg gtgctcttcc ccacgggggc gcacaccgat ctctcgcgtg
     4681 ccgaggggcc gtttgacgtg ctcaagcgcc cggcagcgct ccccgcggag tggatcggtg
     4741 gttcgccctg tcgcccgcag cactcgttcg tcgatgtgag cgatggtcag cgcggcatcg
     4801 ccattctcaa ccagggccta cccgagtacg aggtgaagcg cgatgtggac cgcaccatcg
     4861 cgctcaccct cctgcgctgc gtcgggcggc tcagtgatgg cgatgatgct cccgggatcg
     4921 acccgacgcc cggcgcccag tgcctcgggc ggcacgtctt tcgctacgcc ctcatgccgc
     4981 acgccggctc ctgggaagca gcgcgcatct gggagcaggc ccacgccttc aacgcgccga
     5041 ttcgacccgt gcagaccgat caggcggatg gcgaccttcc cgccaacgcc tccttcgtgg
     5101 agatccgccc ggcccacctg gtcctgagcg ccatcaagcg tgccgaggat gaggacgccc
     5161 ttgtgctccg cttctacaac atcaccaacg caaacgccgc cggcgaggtg atcctccact
     5221 tccccatccg cgccgcctat cgcgcgaatc tgaacgagga gaaggcccag ccactggatg
     5281 tgaacgatgg ccggcggatc cgcgtgccgg tccgtggcaa ggagatcgtg acgctcctcc
     5341 tctaccggta gccgggtgcc cccgcgctca ctcgcctcgt gccactcggt ccatatgata
     5401 gatgaggcga ggggtccagc ggaggtgact gatggagcgg atgcctccat cgaaggcggc
     5461 cttcatggcg cgcatggcga tatcggcata ctgcctgtca cccccgaggc ggtaggcgta
     5521 gaagagcatc cccgcggtca tgtcgttgtt ggcgatgtag cccttcatct tcgggcaaga
     5581 ggtgtagcgg aagccgttgg catcctccac ccagagctcc gcaaccacct gcttcgctcc
     5641 gccaaggagc gcctgggcaa cgcgctgatc cgacgtctcc cggtagtact ctgccagacc
     5701 gttcgcgagc acgcccatca tgaagttcgc ctcgccgcga tgccggggca tgcagtagca
     5761 gtggccgggc accatctggc ggtgccagcc gccgccgggc gtctgccgct ccagggtgcg
     5821 ctcgacgata atccgcgcgg cattcaggta gtaagggtcg ttcgtgagcc ggtatgtcgc
     5881 cagggtaagc aggaggtgcc agccattgtc gcggcagttg ctgtagtcgt agttgttcag
     5941 gtaggcgccg ccatagtaat ccgccacgag cgccgcgttc tcggctgccg ttgggtcgcc
     6001 gctcaggaag taccagtcac agaagccctc ggtccaggtg tggctggggg tgaagccgcc
     6061 tccggggatg ccgctcccct catactgctc gcggaagtag cccccggtat gccccatcgc
     6121 atggatccac acgccgcccg ctctcggacc gtggtgacag gtatccacgt cgatggcgtg
     6181 acgcgccgcc cggtcaccca ggaagtacca ccgccggtcg cccgtgcgga tgaactgcaa
     6241 caggaaggcg tggtggtggt catactcgcc gttcgaccag ttcaccttgc gctcgccaaa
     6301 ctggtccccg aagttcagca ggccatagcc gcgcgtggca tcccgatggc gcaggtagtt
     6361 ttcatagacc ccctcgcata ccttctcata ctccggggag cgcccggcgg tggcggggag
     6421 gatctcgccg aagacgaggg tatcgcagta gcgctccggg gtgcagacgg cgatgagcgg
     6481 ctcctggaac gcctgcgaca cgtgccgggc cgcgtcgcca tccgcctggc ccggatgaaa
     6541 tgccaagagg aggtcgtgct gcttctgcac accacggcgc acgcgatact ttccgcgcat
     6601 cagcgcgaag tagagcttga tctcgtccag cttgctgcac ccatcgtagg taccctcggg
     6661 gaaatccggg cagagatcga tcttcagccc ctccgcgtcg acgccgattc ccttcggata
     6721 gagctgccag aagtcgcgca cggcggccgt cactccccac cggccgttgc tcaggtcgat
     6781 ccagccgtcc gcgcgttcgg tcttcacccc cgccggcgcg gccggctcca gcgtgtagga
     6841 gtcatcccgg agctggcgca gcgagaga
//
LOCUS       DAOSCV010000091         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7136_length_6834_cov_3.610562, whole genome shotgun sequence.
ACCESSION   DAOSCV010000091 DAOSCV010000000
VERSION     DAOSCV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7136_length_6834_cov_3.610562"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PLY56_03515"
     CDS             <1..798
                     /locus_tag="PLY56_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20579.1"
                     /translation="KDGHDTIPHYIKASIVTPDDWKRAKEERFRRDDPARRVDVEALK
                     RAYPPDRDYPLGVHCGSMIGVIRNMLTFEGLAYATYDYPDMVEDMVETACVLVEDFLD
                     QVLGEIDFDFASGWEDICFKNGPLITVEFFERVVVPRYKRISKRLRAHGIDLWYTDCD
                     GDVRPLLPGFLEGGINCLFPFEVNGCAHPAEVLNEYGGELRIMGGVDKIQLGRGPEAI
                     KAYLETLVPLVERGGYIPFCDHRCPPNVKVEDYLYYLDLKERMFGMK"
     gene            complement(837..1577)
                     /locus_tag="PLY56_03520"
     CDS             complement(837..1577)
                     /locus_tag="PLY56_03520"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ20580.1"
                     /translation="MVWPWLQRLGEGLVDLVFPPNCVACHGPAPEYLCASCIASIRRV
                     ERPLCHCCGRPLDLLSVSPESSFRERALCARCRALPVEFDRCRAYGWYEGALREAILA
                     LKYHGKTAVIPALAELLRRALDEDPVLSRARTLVPVPLHPRRRRERGFNQAELLARAL
                     AAQSGRALRCDLIVKTRYTRPQVGLNAEQRRENLRDAFAVQGEAAPAGPILLIDDVTT
                     TGATFHACACALRAAGATTIYALALARD"
     gene            complement(1643..2608)
                     /locus_tag="PLY56_03525"
     CDS             complement(1643..2608)
                     /locus_tag="PLY56_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ20581.1"
                     /translation="MMLNSSATALEPSPVLIVGSVAFDSVETPLGREENVLGGAAVYA
                     SVSASFFAPVQIVGVVGTDFPKEHVEFLRARGIDTEGLQVAEGKTFRWSGYYDYDLNQ
                     AHTRQTDLNVFADFHPRLPESYRDARIVFLANIDPDLQLEVLEQVRDPRLVVCDTMNF
                     WIERKPERLREVLRRVNLVFMNDAEARQFCGTFSLLRAARDLLAMGPDGVIIKKGEHG
                     ALLFTKGKHFSAPSYLLEQVADPTGAGDTFAGGFVGYLAYTGDLSEANLRKATVYGSV
                     MASFNIQDFSLNRLRTLTQEEIAARYEEFKEIAFFEAISASGETA"
     gene            complement(2577..3077)
                     /locus_tag="PLY56_03530"
     CDS             complement(2577..3077)
                     /locus_tag="PLY56_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20582.1"
                     /translation="MIDRRFLFVDAEMLAETVIARCTDPEDGRLGELLRAAPPDVARQ
                     AVRRPDVQFALAIRISATEEERHSWTAMLLRVLRQVDSEAAAAVAWECDRAALARTLS
                     YAEEYTANQVNLLARLYACNPEVGGRVLQRCGSLFREFLVQSAFPYLDLPEELVDDVE
                     QFCDRA"
     gene            complement(3067..3825)
                     /locus_tag="PLY56_03535"
     CDS             complement(3067..3825)
                     /locus_tag="PLY56_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HOJ20583.1"
                     /translation="MLETIELFGVRVHRLTMDDAVAVVEGFLQEGKARQVVTADTSML
                     VMAQRDPELREIINTAALVTPDSFGVLWAARHLGLPLQERVTGVDLMERICARCAESG
                     HKVFLFGAAPGVADEAAERLKERFPGLQVAGTRHGFYKPDEEEGIAAEIAASGADALF
                     VALGIPRQEKWIARSLDRTGVSVAIGVGGSFDVFSGRVKRAPRWVQRCNLEWLYRLCK
                     NPRKIGKVKTLPVLVMMTLGTLARGDGKRARRDR"
     gene            complement(3947..5203)
                     /gene="ahcY"
                     /locus_tag="PLY56_03540"
     CDS             complement(3947..5203)
                     /gene="ahcY"
                     /locus_tag="PLY56_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ20584.1"
                     /translation="MQHDVKDLSLAPKGRLRIEWAAAEMPVLALIRERFEREKPLKGL
                     RLVACLHVTTETANLAITLKAGGAEVALCASNPLSTQDDVAAALVADYGIPTFAIKGE
                     DHETYYRHIHQALDLKPHLTMDDGADTVSVIHSERQELMAEIIGGTEETTTGVIRLRA
                     MEKDGVLRYPIIAVNDAETKHFFDNRYGTGQSTIDGILRATNRLLAGTKFVVAGYGWC
                     GRGIAMRARGMGAQVIVTEVDPIRALEAVMDGYLVMPMAEAVKQGSIFCTATGDVSVL
                     RQEHFEAMRDGAILCNSGHFNVEIDIPALESMAKSKRTVRDFVDEYTLPNDRRIYLLG
                     EGRLINLASAEGHPASVMDMSFANQALCAEYMARNASQLERKVYAVPREIDRNIAALK
                     LHSMGVAIDTLTSEQREYLASWEMGT"
     gene            complement(5279..6325)
                     /locus_tag="PLY56_03545"
     CDS             complement(5279..6325)
                     /locus_tag="PLY56_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOJ20585.1"
                     /translation="MILAAGVGSRLDPLTRTVPKPMVPVVNRPVLEHLVDLLVRHGCS
                     RVVMNTHYLAERIEEHFGNGEAFGTEVIYSREERLLGTAGGVKRVAEHFDDTFVVIGG
                     DDLTDVNLSEMIDFHRERKALVTIGLSVVDDPSQFGVVLLGENGAVRRFVEKPGRGEV
                     FSNRVNAQVYILEPEVLKMIPDGVPYDFGKQLFPRLLREGYPFYGYEMHSYWCDIGSL
                     TQYRRANLDALNGVAPVNFTVPEVERGLWVGDGVRIAPSAEIGRPVVLGDGCIIESGA
                     RVMENSILGAGVVVEEGAIVRHSILWEGAEIQHGTILERCVVGRHCRVKSNAAIFDGI
                     VVDPGRSEAPSLGR"
     gene            complement(6395..>6834)
                     /locus_tag="PLY56_03550"
     CDS             complement(6395..>6834)
                     /locus_tag="PLY56_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ20586.1"
                     /translation="NSRLDALQAAILDAKMNALEAWNDARRANAAYYREALAGLPLVL
                     PIEVEGNHHIYHQFTVRVPKGEVDREALRGHLQARGVGNAVYYPLPIHLQDAYLRWSE
                     GPGSLPESERAAEEVLSLPVYPEMTAEQRAYVADSVRSFFTGR"
BASE COUNT         1225 a         2381 c         2084 g         1144 t
ORIGIN      
        1 aaggatgggc acgacactat tcctcactac atcaaggcga gtatcgtcac cccggacgac
       61 tggaagcggg cgaaggaaga acgctttcgc cgcgacgacc cggcgcgccg ggtggatgtg
      121 gaagcgctga agcgggccta cccgcccgac cgcgactacc ccctgggggt gcactgtggc
      181 tcgatgatcg gcgtgatccg caacatgctc accttcgagg gcctggccta tgccacctac
      241 gactaccccg atatggtcga ggatatggtc gagaccgcgt gcgtcctcgt tgaggacttt
      301 ctcgatcaag tgctcggcga aatcgacttc gattttgcgt ccggctggga ggatatctgc
      361 ttcaagaacg gcccgctgat caccgtggag ttcttcgaac gcgtcgttgt gccccggtac
      421 aagcggatca gcaagcgcct gcgcgcccac ggcatcgacc tgtggtatac cgactgcgat
      481 ggcgacgtgc gccccttgct ccccggcttc ctggagggag ggatcaactg cctcttcccc
      541 ttcgaggtga acgggtgcgc gcatccggca gaggtcctca acgaatatgg cggcgagctc
      601 cgcatcatgg gtggggtgga caagattcag ctgggccggg gtcccgaggc gatcaaggcc
      661 tacctcgaaa cgctcgtgcc cctcgtcgag cgcggtggct acatcccatt ctgcgaccac
      721 cgctgcccgc ccaacgtgaa agtggaggat tacctctatt acctcgacct aaaggagcgc
      781 atgtttggaa tgaagtaggt ggttcgcgcc gaccggccca gctggcgcgc aaccgctcag
      841 tcgcgcgcga gagcgagcgc gtagatcgtc gtggcgccgg cagcgcgcag ggcgcacgcg
      901 cacgcgtgga aggtggcgcc cgtcgtcgtg acgtcatcaa tgaggaggat ggggccggcg
      961 ggcgcggcct cgccctgaac tgcgaaggcg tcgcgcaagt tctcgcggcg ctgctccgcg
     1021 ttgaggccca cctgcggccg cgtgtaccgg gtctttacga tgagatcgca ccgcagtgcc
     1081 cggccgctct gcgcggccag cgccctcgcg agcagctctg cctgattgaa cccacgctcg
     1141 cgtcgccggc gcgggtgcag cggcaccggc accagtgtcc gcgcccggct caacaccggg
     1201 tcctcatcca acgcccgcct cagcagctct gccagcgccg gaataacggc cgtcttgcca
     1261 tggtacttca aggcgaggat cgcctcgcgg agcgcgccct cgtaccatcc ataggcccgg
     1321 caacggtcga actccaccgg tagggcccgg cagcgcgcgc agagagcgcg ctcgcgaaag
     1381 ctactctcgg gcgagacgga gaggagatcc agcgggcgcc cgcagcagtg gcaaagcggc
     1441 cgctccacgc ggcgaatcga ggcaatgcaa gaggcgcaca ggtattcggg cgcaggtcca
     1501 tggcaggcga cacaattcgg agggaagaca aggtcgacca ggccttcccc caggcgttgg
     1561 agccagggcc acaccatagc gccctccacg cgggaggctt tccgcgcgcc ggcctcggcg
     1621 ctcgttgctc cagggatgct gctcaggccg tctcgccgct ggccgagatt gcctcgaaga
     1681 aggcgatctc cttgaactcc tcgtagcgcg cggcgatctc ctcctgagtt agggtgcgga
     1741 gccggttcag gctaaagtcc tgaatgttga acgaggccat gacactgccg taaactgtgg
     1801 ccttgcgcag gttcgcctcc gaaaggtctc cagtgtaggc gagataacca acgaagccgc
     1861 cggcgaaggt atcgcccgcg ccggtgggat cggccacctg ttcgagaaga taagagggag
     1921 cggagaaatg cttccctttc gtgaagagca acgcgccatg ctcgcccttc ttgatgatca
     1981 cgccatcggg gcccatcgcc agaaggtcgc gcgcggctcg gagcaggctg aaggtgccgc
     2041 aaaactggcg cgcctccgca tcattcatga agacgaggtt cacgcggcgg agcacctcgc
     2101 gcaaccgctc cggcttccgc tcgatccaga agttcatcgt gtcgcacacg accaaccgcg
     2161 ggtcgcgcac ttgctccagg acctccaact gcaggtcggg gtcgatattc gccaggaaaa
     2221 cgatgcgcgc atcgcgatac gactcgggca ggcgggggtg gaagtccgcg aaaacgttca
     2281 gatccgtctg ccgcgtgtgc gcttggttca ggtcatagtc atagtagccg gaccagcgga
     2341 acgtctttcc ctctgcaacc tgcaagcctt cggtgtcgat cccgcgcgcg cgcaggaact
     2401 ccacgtgctc cttcggaaaa tcggtgccga ccacgccgac gatctgaaca ggggcaaaaa
     2461 aggaggccga cacagacgca tacacggcgg cgccccccag cacgttctcc tcgcgtccaa
     2521 ggggagtctc caccgaatcg aaagccaccg agccgacgat cagcacgggc gacggctcaa
     2581 gcgcggtcgc agaactgttc aacatcatcc accaactcct ccgggaggtc gagataggga
     2641 aacgcagatt ggaccagaaa ctcgcggaac aggcttccgc accgctgaag caccctgccc
     2701 ccgacctccg gattgcacgc atagaggcga gccagcaggt tcacctggtt cgccgtgtac
     2761 tcttcggcgt aggagagcgt tcgcgctagt gcggcccgat cacactccca ggcgacggct
     2821 gctgccgcct cgctatccac ctgccgcagc acgcgaagga gcatcgccgt ccaggagtgt
     2881 cgctcctcct cggtggcgga gatgcggatt gccagggcaa actgtacatc cggacggcgc
     2941 acggcctggc gcgccacatc gggcggtgcc gcgcgcagca actcgcccag ccggccatcc
     3001 tccggatcgg tgcagcgggc aatcaccgtc tccgcgagca tctcggcatc aacgaacagg
     3061 aatctcctat cgatcacggc gcgcccgctt cccatcgccg cgcgcgagtg tcccgagggt
     3121 catcatcacg agcacgggca gcgtcttcac cttgccgatc ttgcgcgggt tcttgcagag
     3181 ccggtagagc cactccaggt tgcagcgttg cacccaccgg ggcgcgcgct tcacgcggcc
     3241 agaaaaaacg tcgaagctgc cccccacgcc aatggccacg gagacgccgg tccggtccag
     3301 ggagcgcgcg atccatttct cctggcgggg gatcccaagg gcaacgaaaa gcgcgtcggc
     3361 gccggaggcc gcgatctcgg cggcaattcc ctcttcctcg tccggcttgt agaaaccatg
     3421 ccgggtgccg gccacctgca gtccggggaa acgctctttc agccgctccg ccgcttcatc
     3481 cgcgaccccg ggcgcggcgc caaagagaaa caccttgtgc ccgctctcgg cacaccgggc
     3541 gcagatccgc tccatcaggt ccacaccggt cacgcgctct tgcagtggca agcccaggtg
     3601 acgcgctgcc cagaggacgc cgaagctatc gggcgtcacc agagcggccg tgttgatgat
     3661 ctcgcggagt tccggatccc gctgcgccat caccagcatg gaggtatccg cggtgaccac
     3721 ctggcgcgcc ttcccctcct gaaggaagcc ttccaccacc gccaccgcgt cgtccatcgt
     3781 cagccggtga actcgaaccc cgaacagctc aatcgtctcc agcatcgccc ccctgattca
     3841 gcgggcagga ggcaacggcg cagcccgcca ccgctgcttc ctgcccgtgc atcgcatccc
     3901 ccacctgcct cagcacgcta tccggcccat tcgcgtccga gccagctcag gtgcccatct
     3961 cccaagaggc aaggtattct cgctgctcgg aggtaagcgt atcgatcgcc acgcccatcg
     4021 agtgcagctt cagcgccgcg atgttccggt cgatctcgcg cggcaccgca tacaccttgc
     4081 gctcgagctg gctggcgttg cgcgccatat actccgcgca cagcgcctga ttggcaaagc
     4141 tcatatccat gacgctggca ggatggcctt ctgccgaggc caggttgatc aaccggccct
     4201 cgccaagcag gtagatacgc cggtcgttgg ggagggtgta ctcgtccacg aagtcgcgca
     4261 ccgtccgctt cgatttcgcc atgctctcca gcgcgggaat gtcaatctcc acgttgaaat
     4321 ggccagagtt gcacagaatg gcgccatcgc gcatcgcctc gaagtgctcc tggcgcagca
     4381 cggacacatc gcccgtggcc gtgcagaaga tgctcccctg cttcaccgct tccgccatcg
     4441 gcatcaccag gtagccatcc atcaccgcct caagcgcccg gatcggatcg acctcggtga
     4501 cgatcacctg ggcacccatg ccccgcgcgc gcatcgcgat cccgcgcccg caccagccat
     4561 agccggcgac gacgaacttt gtgcccgcga gcaggcggtt cgtggcgcgc aggatgccgt
     4621 caatcgtgct ctggccggtg ccataccggt tgtcgaagaa gtgctttgtc tccgcgtcgt
     4681 tcaccgcgat gatcgggtag cgaagcacgc cgtccttctc catggcgcgc agccggatca
     4741 cgcccgtggt ggtctcctcg gtgcctccga tgatctccgc catcagctcc tggcgctcgg
     4801 aatggatcac cgagacggta tcggccccgt catccatcgt gagatggggc ttcagatcca
     4861 gcgcctgatg gatatgccgg tagtaggtct cgtggtcctc gcccttgatg gcgaaggtgg
     4921 ggatgccgta atccgccacc agcgccgccg ccacgtcgtc ctgcgtggag agcgggttcg
     4981 atgcgcacag ggccacttca gcgccgccgg ctttcaacgt gatcgccaag ttggccgtct
     5041 ccgtagtgac gtgcaggcag gccaccaggc gcaggccctt cagcggcttt tcccgttcga
     5101 agcgctcgcg aatcagcgcc aggacgggca tctccgccgc tgcccactct atccgcaggc
     5161 gtccttttgg cgccaggctc agatccttga catcatgctg catttcgtta cccctcagtc
     5221 gcgtgccggg cagatgccgc ccgctcttct caataggcgc ccgcttgcca atgcgccctc
     5281 atcgccccaa cgagggcgcc tcggatctcc ccggatccac cacgatccca tcgaagatcg
     5341 cggcgttgct cttcacccgg caatggcggc ctacgacgca ccgctccagg atcgtgccgt
     5401 gctggatctc cgcgccctcc cagaggatgc tatgccggac gatggccccc tcctcgacca
     5461 ccaccccggc tcccagaatg ctattctcca tcacgcgcgc gccagactcg atgatgcacc
     5521 catcgcccaa caccacggga cgcccgattt ccgcgcttgg cgcgatgcgc acgccgtcgc
     5581 ccacccacaa cccgcgttcc acctcgggca ccgtgaagtt cacgggcgcc acgccgttga
     5641 gcgcatcgag gttggcgcga cggtattgcg tcaggctccc gatatcgcac cagtaagagt
     5701 gcatctcgta tccgtagaag ggatagcctt cgcgcaacaa ccgtgggaag agctgcttgc
     5761 cgaagtcgta gggcacccca tccgggatca tcttcagcac ctcgggctcc aggatgtaca
     5821 cctgtgcgtt cacccggttg ctgaagacct cgccacgccc tggcttctcg acgaagcggc
     5881 gaaccgcgcc attctcgccc agcagcacca cgccaaactg cgaggggtcg tccaccacag
     5941 agagcccgat cgtcaccagc gccttgcgct cgcggtggaa atcgatcatc tccgagaggt
     6001 tcacgtcggt aaggtcatcg cctccgatga cgacgaaggt gtcatcgaag tgctccgcga
     6061 cgcgcttcac gccgcccgcg gtgcccaaca ggcgctcctc gcgcgagtag atcacctccg
     6121 tgccgaacgc ctcgccgttc ccaaaatgct cctcgatgcg ttcggccaga tagtgggtgt
     6181 tcatgacaac ccgggagcac ccatggcgca ccagcagatc caccaggtgc tcgaggaccg
     6241 gccgattgac taccggtacc atcggcttgg gcacggtgcg cgtcaacgga tccaggcgcg
     6301 agcccacgcc agccgccaga atcatcgctt tcacggaacg ctcctcccct cgggccgcgc
     6361 gcgttcctgc gctgcgcacg atccgcggcg cccgttagcg accggtgaag aaggatcgca
     6421 cgctatccgc cacgtacgcg cgctgctcgg cggtcatctc gggatagacc ggaagcgaga
     6481 gcacttcttc cgcggcacgc tccgactcgg gcaggctccc cgggccctcg ctccagcgga
     6541 gataggcatc ctgaaggtgg atggggagag gatagtagac ggcattcccc acgccgcgcg
     6601 cctgcagatg cccgcgcaac gcctcgcgat ccacctcgcc cttgggcacc cgcaccgtga
     6661 actggtggta gatgtggtgg ttgccctcta cctcgatggg aagcaccagg ggcaggcccg
     6721 ccaacgcctc gcgataatag gccgcgttcg cccggcgggc gtcgttccag gcttccaggg
     6781 cgttcatctt tgcgtcgaga atggcggcct gcaaggcatc gagccgcgag ttgt
//
LOCUS       DAOSCV010000092         6832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7143_length_6832_cov_3.660322, whole genome shotgun sequence.
ACCESSION   DAOSCV010000092 DAOSCV010000000
VERSION     DAOSCV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6832
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7143_length_6832_cov_3.660322"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLY56_03555"
     CDS             complement(<1..991)
                     /locus_tag="PLY56_03555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ20587.1"
                     /translation="MSEPVLPKRVVFVCAAYLPRITGVATAINAWARQLLPLGVRVTV
                     VAPEHTPHTFYPLHSGPGELDIVRLPGQPAPFVLGIQRARTRGVAWDELLASLQGENA
                     IIHGQDVHSAGEIATQIGRRCRLPVMLHAHYPLLDSDPADWLTFSPGMLGRKAVNAVI
                     SGILAHKARATCRRANLIAAVSPYVQEMLAKCGISNTVVLPCGVDLPADLPAVDIRRR
                     HGIPQDSPIFLYTGRMDPDKRIGDLLEVAAILRQREPRARLLLVGGGHYLERYIRRAE
                     ALRLGDTVVFAGWASYQDIWAYYAQADLFLLANAHEALGLVTLEAQAMGLPVVG"
     assembly_gap    1071..1080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1217..3400)
                     /locus_tag="PLY56_03560"
     CDS             complement(1217..3400)
                     /locus_tag="PLY56_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ20588.1"
                     /translation="MRYLTALLLLIPATLLFARLTRAEPLVTPGYAVNYYLSEIDGSY
                     QPYGVYIPDPYRPDEPHPVVLHLHGFGGRLGGFSRWQREWADSNGWILAYPDGRGSNN
                     YDGIGEDDLFRVLDDLKARYHVDEDRIYLTGGSMGGHGSFRMPLRYPHIFTAGAPIAG
                     WTDYREFYPHFYEQAEGPKLPEYVDPSRRPILETASSLWQVENGRYNWLLIGYGTWDY
                     VNPPSNAEQVVQRAREFGFQGVTARGDWGGHGAGYDTEEIYNWFLGKARVRKPENPTY
                     VTNTVKYNRGHWLRIDRLRVLNQWARLSARARRQVIQVEASNVARYTLLDEGNPVDFT
                     KNVTVYTNGLLSYQGVPNGPLTLEARTDERHRVVGWGTPQPPLRHGPAKRHGLQGPIA
                     DAFLSRFVVVYGTTGTRAETRRNYEDALRFCAEWNNWMTLHWGWEKPPKERMRNWWEA
                     PYPMRPGAYTPENQPLLRPIRDTELTDEQIASANLLLFGDLKSHATIARIAGELPLTL
                     TRSGVIAGNRTYAGPSINYVFVAPNPLNPNRYVVVSRGYLSSRIDPYSSSPSTVGKDL
                     EALPFFWPDYVIWDGNRKPAPTVQSPFVYLPETYLEAGYFDDGWALDANPPGTTAFID
                     GDGQEGVYASPVEIALVAEDQTGGFGVARTEYRLNGGPWDEYTGPILVESPGNHVLQF
                     RSVDRCGRFLYEVNDGHSRGYDAPGNVEAVRSVSFRVTAKRSASR"
     gene            complement(3566..5566)
                     /locus_tag="PLY56_03565"
     CDS             complement(3566..5566)
                     /locus_tag="PLY56_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20589.1"
                     /translation="MQADPTRSYSGPHPQQTPPAVAEKASRLRGRAVLLGLALIPPNF
                     YWIVNLEVNRYSFATYAAPFYNVIFTLFLLTLLNQVVRRWRPALALNRAELLTIYAML
                     SIASALNSHNCMQILLGIMGHGFYFATPENAWEDLFLPHLPSWLTVSDRAVLEGYYSG
                     HSSFYQPEVLRAWAVPIVCWTGFGVVLLGTMLCINVLFRRQWVEAERLTYPIVQLPLE
                     MTAEEGGFWRNRLMWIGFAVAFGVTLLNGFAYLFPSIPSVPIKRQNIGHLFTQRPWSA
                     IGAVNISFYFFAIGLTFLMPLDISFSCWFFYWFHKMQLVLGSATGWSYQARPGGGFDS
                     VFPYANSQAFGAYMAVFLMGVWGARHHFARVWRTARGLPGGLDDSAEPLRYRAALIGV
                     AVGGLALIGFGIAAGLTWWVAIVYFAVYFALAVLVCRIRAEVGLPVHDMHVMGPHSIL
                     ITTCGTQALGARNLTIFSLFYWFNRTYASHPMPHQMEAFKLAERTGASGRRMAMALMA
                     AAAIALPCAFWLLLDNAYRNGCATGRVEVWGTGFGREVYNRLQSWTKNPMPVNYTSMG
                     FIGAGFGFAMLLGIARLRFSWFPFHPLAYAVANSWGIAQLWLPMLIGSTAKLAILRFG
                     GLKSYRAAIPFFLGLILGEIVAGSFWSLLGVCAGFRTYDFWP"
     gene            complement(5716..>6832)
                     /locus_tag="PLY56_03570"
     CDS             complement(5716..>6832)
                     /locus_tag="PLY56_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20590.1"
                     /translation="LVAGLVPTEATELRRGNVVTIGPGQVVDDDLVIAAQRVVVEGTV
                     RGDLIVTANSLRVPGRVTGNLLGLTQSLEVPGTVDGSLRAATQIATIAGQVGRNLSIA
                     AQNTTIARGARINRDAHIAAQSVELNGAVGRQAGLAAQSASIGGTVGQHLRFDGNNLT
                     IDPAARIGGDLLVRSPRQAEIAPGATIAGQVRRLPVEPRRERRPTPGPGIGLLWFNLL
                     LAVALFIVGSIGLALAPRFLRTAADQLREKPGWSILWGILLFIVVPIAALVVGFTIVG
                     IPLSLLAWALWGFAVLFAAIPVALFLGREFTGWLRRGAPLSPYANLAIGLALIALVAA
                     IPVVWLFVWLAVVFFGVGMFALAVYDLMEQRRGAAPA"
BASE COUNT         1326 a         2196 c         2235 g         1065 t
ORIGIN      
        1 agccaacgac cggcagcccc atcgcctgcg cctcgagcgt caccaaccca agcgcctcat
       61 gggcattggc caggaggaaa agatctgcct gagcatagta ggcccagatg tcctgatagg
      121 aggcccagcc ggcgaagacc accgtatcac caagacgcag cgcctccgcg cgccggatat
      181 accgctcgag atagtgcccc cccccaacca ggaggaggcg cgcgcgtggc tcccgctggc
      241 gtaggatcgc cgccacctca agcaggtcgc caatccgctt gtcggggtcc atacgccccg
      301 tgtagaggaa gataggactg tcctgcggga tgccatgccg cctgcggata tcgacggcgg
      361 ggaggtcggc gggcagatcc accccgcagg gcaacaccac ggtgttgctg atgccgcact
      421 tcgcgagcat ctcctggacg tagggactga ccgcggcgat cagattcgct cgccggcacg
      481 tggcgcgcgc tttgtgcgcc aggataccgc tgatgacggc attcactgct ttgcgcccga
      541 gcatgcctgg cgagaaagtg agccagtccg caggatcgct gtccaagagg ggataatggg
      601 cgtgaagcat caccggcagg cggcagcgcc ggccaatctg ggtggcaatc tccccggcgg
      661 agtgaacatc ctgcccatgg atgatcgcgt tttcgccctg caggctggcc aacagctcgt
      721 cccatgcaac accacgcgtg cgcgctcgct ggatgcccag gacgaagggg gccggctgtc
      781 ccggcaggcg aacaatgtcg agctcacctg gaccggagtg cagggggtag aaggtgtgtg
      841 gggtgtgctc cggcgcaacg accgtcactc gcactcccag tgggagaagc tggcgcgccc
      901 atgcgttgat cgccgtcgct acccctgtaa tccgagggag atacgcggcg caaacaaaaa
      961 cgaccctctt ggggagaacc ggctcgctca cgattgtgag tataccaccg ggtccctttt
     1021 actgtcaaca gcgcctcttg cctaatcaca tggcgagtcg aggctcgcgc nnnnnnnnnn
     1081 acccactgat gaagaggacg gacatggatt gggaaggcgc gggcgccgag ggcctgcata
     1141 gacgcaggcg ttacgaatgt ttggcagaaa gacgaacctt ccgcattcgc cctggatccg
     1201 gtgtggcaga cagcccctac cggctcgcgc tccttttggc ggtcacccgg aacgagacgc
     1261 ttctcaccgc ctccacgttg ccaggggcgt cgtagccgcg gctatggcca tcgttcacct
     1321 catacaggaa gcgcccgcag cgatccaccg atcgaaactg gaggacgtga ttgccgggcg
     1381 actctaccag gatcgggccg gtgtattcat cccaaggccc gccattcagg cggtactctg
     1441 tgcgcgcgac cccgaagccg ccggtctgat cctcggccac cagggcgatc tccactggac
     1501 tggcgtagac accctcttgg ccgtcaccat cgatgaaggc ggttgtgccc ggcgggttgg
     1561 cgtccagcgc ccatccgtca tcaaagtagc cggcctccaa ataggtctct ggcaggtaga
     1621 cgaaaggcga ctgcaccgtg ggcgccggct tgcggttccc gtcccaaatg acgtagtctg
     1681 gccagaagaa ggggagcgct tccagatcct tcccaacggt ggagggcgag ctggagtagg
     1741 gatcaatccg gctggagagg tagcctcggc tcacgacgac gtagcgattg gggttcaaag
     1801 ggttgggcgc cacgaagacg tagttgatgc tcggcccggc atacgtccgg ttgccggcga
     1861 tcacgccgct cctggtgagg gtgagcggca gctccccggc aatccgggcg atcgtcgcgt
     1921 gcgacttcaa gtcgccaaag aggaggaggt tggcgctggc gatctgctcg tcggtcagct
     1981 ctgtatcccg gatcgggcgc aggaggggct ggttctccgg ggtgtacgct cctggtcgca
     2041 tggggtaggg tgcctcccac cagttgcgca ttcgctcctt gggcggcttc tcccagcccc
     2101 agtgcagagt catccagttg ttccactcgg cgcaaaagcg gagggcatcc tcatagttgc
     2161 ggcgcgtctc ggcccgtgtg ccggtcgttc cgtatacgac gacaaaccgc gagaggaagg
     2221 cgtcggcgat cggcccctgc aggccgtggc gttttgccgg gccgtgccgg agcggcggct
     2281 gtggcgtgcc ccacccaacc acgcggtggc gctcgtcggt gcgcgcctcg agagtgagcg
     2341 gtccgttggg cacgccctga tacgagagga gtccgttggt gtagacggtc acgttcttgg
     2401 tgaaatcgac cgggttcccc tcatccagga gcgtgtagcg ggcgacgttg ctcgcctcaa
     2461 cctggatcac ctgcctccgg gccctggccg acagccgcgc ccactggttg agcacccgca
     2521 ggcggtcaat ccggagccag tgcccccggt tatacttgac ggtgttggtc acataggtgg
     2581 ggttctccgg cttgcggacg cgggctttgc caaggaacca gttgtagatc tcctccgtat
     2641 catagccggc gccgtgcccg ccccaatccc cccgcgcggt gactccctgg aagccgaact
     2701 ctcgggcgcg ctgcaccacc tgctcggcgt tggaaggcgg gttgacatag tcccaagtgc
     2761 cgtagccgat gaggagccag ttgtagcggc cattctccac ctgccagagg gaagaagccg
     2821 tctccaggat cggccggcgt gaaggatcca cgtactccgg gagcttcggt ccctcggcct
     2881 gctcgtagaa gtggggataa aactcgcggt agtcggtcca gccggcgatc ggtgcgccag
     2941 cggtgaagat gtgcgggtag cgaagcggca tgcggaagga gccgtggcct cccatcgacc
     3001 cgcctgtcag atagatccga tcctcatcca cgtgataccg cgccttgagg tcatcgagca
     3061 cgcggaagag gtcatcctcg ccgatgccgt catagttgtt gctgccccgg ccatcggggt
     3121 aagcgaggat ccagccgttg gaatcggccc actctctctg ccaccgactg aacccgccca
     3181 ggcggccgcc gaagccgtgg aggtgcagga ccaccgggtg gggctcatcg ggccggtacg
     3241 gatcgggaat atagactccg tagggctggt aagagccatc gatctcggag aggtagtagt
     3301 tcaccgcgta cccaggcgtg accaggggtt ccgcgcgcgt gaggcgggcg aagagcaacg
     3361 ttgccggtat cagcagcagc agagcggtca ggtatcgcat ggcgaggatc aacgatcggc
     3421 agggcatgcg tattctggca gcgcccggca cccgcgccgg agcaacgcca cctccaactc
     3481 ccgagtcgtc atcgtttgct ccctgttcga cgtgcgccac caggcacctg ttttcacgtc
     3541 ggcatccacc ccacgcgcat tccacttaag gccagaagtc gtaggtgcgg aagcccgcgc
     3601 acacccctaa gagactccag aaagagccgg ccacgatctc cccgagaatg agccccagga
     3661 agaaggggat ggccgcccgg taactcttca ggccaccaaa gcggagaata gccagcttcg
     3721 cggtggagcc aatcaacatc ggcagccaga gctgcgcgat cccccaggag ttggccaccg
     3781 cataggcgag cgggtggaac ggaaaccacg agaagcgcag gcgcgcgatc cccagcagca
     3841 tcgcaaagcc aaacccggcg ccaatgaacc ccatcgaggt gtagttgacc ggcatcgggt
     3901 tcttcgtcca cgattgcagc cggttataca cctccctgcc gaagccggtg ccccacactt
     3961 ccacgcggcc cgtggcgcat ccgttgcgat acgcattatc gagcagcagc cagaaggcac
     4021 atggcagggc gatagctgcc gcggccatca acgccatcgc catgcgccgg ccagaggcgc
     4081 ctgtgcgttc cgccagcttg aacgcctcca tctgatgcgg catggggtgc gaggcatagg
     4141 tgcggttgaa ccagtagaaa agcgagaaga tggtcaggtt gcgtgccccc agggcctgag
     4201 tgccgcacgt cgtgatcaaa atgctgtgcg ggcccatcac gtgcatatcg tggacgggaa
     4261 gccccacctc ggcacggatg cggcagacca gcaccgcgag cgcgaagtag acagcaaagt
     4321 agacgatggc cacccaccat gtgagcccgg cagcaatccc gaagccaatc agcgccagcc
     4381 cgccgacggc caccccgatg agcgcggcac gatagcggag gggctccgcc gaatcgtcga
     4441 gccccccagg gaggccgcgt gcggtccgcc agacccgagc aaagtggtgc cgggcgcccc
     4501 atacgcccat caggaagacc gccatatagg ccccaaacgc ttgcgagttg gcatacggga
     4561 agacggagtc gaaaccgccg ccgggccgtg cctggtagct ccagccggtg gcgctgccca
     4621 ggacaagctg catcttgtgg aaccagtaga agaaccagca ggagaaagag atgtccagcg
     4681 gcatcaggaa ggtaaggcca atggcaaaga aatagaagga gatgttcacg gcgccgatgg
     4741 cgctccacgg gcgctgggtg aagaggtgcc cgatgttctg acgcttgatc ggaaccgacg
     4801 gaatcgatgg gaaaagatag gcgaagccgt tgaggagcgt caccccgaaa gcaaccgcaa
     4861 agccgatcca catcaaccgg ttgcgccaga agccgccttc ctcggccgtc atctccagag
     4921 gaagctgcac gatcggatag gtaaggcgct ccgcctcgac ccactgtctg cggaagagga
     4981 cgttgatgca cagcatcgtg ccgagcagca ccaccccgaa gccggtccag cacacgatcg
     5041 gcacggccca cgcccgaagc acctccggct gatagaagga actatgcccg gagtagtagc
     5101 cctcgagcac ggcacggtcg gagaccgtaa gccaggaggg cagatgcggc aggaagagat
     5161 cctcccaggc gttctcggga gtggcgaagt agaagccgtg ccccatgatc ccgagaagaa
     5221 tctgcatgca gttgtgcgag ttgagcgccg aggcgatgga gagcatcgcg tagatggtga
     5281 gcaactccgc ccggttgagc gcgagcgccg gtcgccagcg gcgcaccacc tgattcagga
     5341 gcgtgaggag gaagagcgtg aagatgacgt tgtaaaaggg cgccgcgtag gtggcgaagg
     5401 agtagcggtt gacctccaga ttgacgatcc aatagaagtt cggcgggatc agcgcgaggc
     5461 cgaggagcac ggcgcgaccg cggagacgtg aagccttctc cgccaccgcc gggggcgtct
     5521 gctgcgggtg tggacccgag tatgagcgcg tcggatcggc ttgcatgcca ctctattcgc
     5581 ggttggggga tgcctccctc ctgcgcttcc agggcatgtc actctttctt gcagagcacg
     5641 cggcggccct gccgggcagg gccgccacca tcctctcgag gagcacccgg gacacgccag
     5701 atctgtctga ggcactcagg cgggcgctgc tccccggcgt tgctccatta ggtcatacac
     5761 agcgagggcg aacatcccca ccccgaagaa gacaaccgct agccacacga atagccacac
     5821 gacgggaatc gcggcgacga gggcgatcag cgccaggccg atggccaggt tggcgtaggg
     5881 agagagcggc gccccgcggc ggagccaccc ggtgaactcg cgccccagga aaagcgcgac
     5941 cgggatggcg gcgaacagca ccgcgaaccc ccacagcgcc catgccagga ggctgagcgg
     6001 aattcccacg atggtgaagc ccaccaccag cgccgcgatg ggaacaacga tgaagagcag
     6061 gatgccccac aggatgctcc accccggctt ttcccgcagt tgatctgcgg ccgtgcgcag
     6121 gaagcgcggc gcgagtgcca gaccaatcga tccgacaatg aagagtgcca ctgccagaag
     6181 caggttgaac cacaggagtc caataccggg ccccggggtc ggccttcgtt cgcgcctggg
     6241 ctcgaccggc agacggcgca cctgcccggc gatagtcgcc cccggcgcta tctctgcctg
     6301 tcgcggcgag cgcaccaaga ggtcgccccc aatacgcgcc gcagggtcaa tcgtcaggtt
     6361 gttgccgtca aaacggaggt gctgccccac ggtgcccccg atgctggcgg attgcgcggc
     6421 cagcccggct tgcctgccca ccgcgccgtt cagctccacg ctctgggcag caatatgcgc
     6481 gtctcggttg atgcgggcgc cgcgagcgat cgtggtgttc tgcgctgcga tcgatagatt
     6541 gcgccccacc tggccggcga tcgtggcgat ctgggtcgcg gcacgcaggc tgccatccac
     6601 cgtacccggc acctccagcg actgggttag cccaagcaga ttgccggtga cgcgccctgg
     6661 cacgcgcaaa ctgttcgccg tcacgatcag gtcgccgcgg accgtgcctt ccaccaccac
     6721 tcgctgggcg gctatgacca gatcatcatc caccacctgc ccgggcccga tggtgaccac
     6781 gtttccgcgg cgcagctcgg tggcctcggt cggcacgagg ccggcgacca ag
//
LOCUS       DAOSCV010000093         6822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7157_length_6822_cov_3.848234, whole genome shotgun sequence.
ACCESSION   DAOSCV010000093 DAOSCV010000000
VERSION     DAOSCV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7157_length_6822_cov_3.848234"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1059
                     /locus_tag="PLY56_03575"
     CDS             277..1059
                     /locus_tag="PLY56_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOJ20591.1"
                     /translation="MKRCLILTALAGLLVAGASAPGVAALFQVEAGGVSIFTTQPVTM
                     DGTVLVPLRQTLTQLGVTDLTFDPATSEVRFTARGQSVVMEVAASSAMVGGETVALAQ
                     PVQRFAGQIWVPAAFISQIFPDFNITQVRGFRFAPEVTPEITSPIPQTTGPSSVSFNG
                     RTVTYSSPTWYMGNVLMVPVNETLEALGIAAPQFNPGDLQLSFIHEGSIVRMNAQSGF
                     LHIGDQAFPMARPAVERDGILYAPASSMSHLIPGLTFSVPQG"
     gene            complement(1214..2155)
                     /locus_tag="PLY56_03580"
     CDS             complement(1214..2155)
                     /locus_tag="PLY56_03580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ20592.1"
                     /translation="MDRRTFLKGAGTLAATALGLDGIAAKDGRVPRRRLGRTGVELSI
                     IGLGGIVVANTEPDEARRIVVEAYERGVNYFDVAPSYGNAEERLGPALEGLRDKVFLA
                     CKTEKRDRAGAAAELRQSLQRLRTDHFDLYQLHGLRGMDDLEKAAGPGGALEAVAEAR
                     KEGLVRWIGFSAHSAEVALEAMERFSFDTILFPVNFVTWIKAGFGPQVIAAAEKKGMG
                     RLAIKAMALGRMPKGTPRTYPKCWYQPIDDPRLASMALRFTLSQPITAAVPPGDVRLF
                     RMALNAVTERFKPLTKSELAELERVAATADPIFRLAA"
     gene            complement(2285..3385)
                     /locus_tag="PLY56_03585"
     CDS             complement(2285..3385)
                     /locus_tag="PLY56_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20593.1"
                     /translation="MGGHYSGAPGHAPAGAGGSRLRLVFWETTAGCNLECIHCRRLEV
                     ARERMRDDLSTEEALALVESIARAGKPILVLSGGEPLLRPDLFTVARHAVGQGLVVSM
                     ASNGTLITPQVAGQVRDAGIRRVSISIDGADAATHDTFRALPGSFDAALRGLGYLRDA
                     GVEVQINTTIARHNAEQLAGIYELARTVGAVAFHAFMLVPVGCGVQIADDQMLSPARY
                     EEILNEFYDLSRRGELQLKATCAPHYYRIARQRARAEGIAPSGPREGMAAMTRGCLAG
                     TAVCFVSHKGQVFPCGYLPVEAGNVRTRDFAEIWRESPLFRTLREDALGGKCGACEYR
                     TVCGGCRARAYYATGDFLAEEPYCSYLPQPRG"
     gene            complement(3461..5026)
                     /locus_tag="PLY56_03590"
     CDS             complement(3461..5026)
                     /locus_tag="PLY56_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ20594.1"
                     /translation="MDEREARSLVAQVLVLRGTPNWREEEDRLVASGAVLNSQVGGPD
                     IAARCAALRQMAPGPMLLQCDMETGSGFAFDGTQIPPLMALGAAGSEELARAWGYAVG
                     LEGRRLGIDVTWSPVLDVNTNPDNPIINVRSFGEDAGLVSRLGAALTRGMKESGLHPC
                     AKHWPGHGDVAVDSHISLPTVNVTRERLEAVEWLPYRTARAAGLESVMTGHLLVPSLD
                     PENCCTVSPAQISILRDDLGFPGPIFTDSLGMEGLRTTLDSTEAAWRALVAGHDQVLI
                     DYKRPPSESVDAVVEAVRDGSVPEARLRAAASVVTALKARLRENAPLPPAEEIRSHLA
                     SVARAVAEASVTAVGALSERPDLGERPLLIVCDDLKRRGVGIAEEREGQELRGTHPLV
                     RILREKAGFETLVFDEVPTPRDLEAVRASVAASTAVVGATFAHILCYKGEGTRLPGAQ
                     IELWREVAASGKLRAMMLFESPYALADLPAGVPVVVGYGADSFTLTAAAEALAGERRC
                     PGRLPVTVASIGD"
     gene            5959..6306
                     /locus_tag="PLY56_03595"
     CDS             5959..6306
                     /locus_tag="PLY56_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ20595.1"
                     /translation="MLASILRGHGFEVMEADAPSSCEVPTLEPCNILTARQVQVLRAL
                     RRYGSREMTARALGIALKTVDAHLEHIYKRAGVRTRIEALCWACRRGLLDEDRSGCEA
                     SGSRPKASPEALA"
     gene            6513..6698
                     /locus_tag="PLY56_03600"
                     /pseudo
     CDS             6513..6698
                     /locus_tag="PLY56_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
     gene            6677..6822
                     /locus_tag="PLY56_03605"
                     /pseudo
     CDS             6677..6822
                     /locus_tag="PLY56_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561505.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
BASE COUNT         1117 a         2394 c         2177 g         1134 t
ORIGIN      
        1 cctctccaaa gttccgtgcc aggtggcatc tggcacggag cagattctcg ctcgtggcgc
       61 cccagccctg catgacacgg agacggttgc aggatgcccc gcggggcttc cgcacgcccg
      121 tagcacggac agatgcgctg ccagaagcgg cctcggaacg cgcgagaggt ccggccccgg
      181 cggcgtggcg gcggcgtttt ggcgcggaac gggtatgggt atgcgcacat tgcagggagc
      241 ctacaaggaa ctgctttcga aaggagcagg ttgatcatga aacgatgtct gatattgacc
      301 gcgctggccg ggctcctcgt cgcaggagcc tccgcgccgg gcgttgcagc gctattccag
      361 gtggaggcgg gtggcgtctc gatattcacc acgcaaccgg tgacgatgga cggaaccgtc
      421 ctcgtgcctc tacggcagac tctcacgcag cttggggtca ccgatctcac cttcgatccg
      481 gccacaagtg aggtccggtt caccgcccgc ggacagagcg tcgtcatgga agtcgcggcc
      541 tcttcggcga tggttggcgg cgagacagtg gcgttggccc aacccgtgca gcgcttcgcc
      601 gggcagatct gggtgcctgc tgcgttcatc tcgcagatct tccccgactt caacatcacg
      661 caggttcggg gattccggtt cgcgccagag gtgacgccgg agatcacctc gccgatcccg
      721 cagaccaccg gcccgagcag cgtcagcttc aacggtcgaa cggtgaccta ctcatcgccc
      781 acgtggtaca tgggcaacgt gctgatggtg ccggttaacg agacgctgga ggccctgggc
      841 atcgctgcac cgcagttcaa cccgggagat ctgcagctct cattcatcca tgagggctcg
      901 attgtccgga tgaacgccca gagtggcttc ctccatatcg gggaccaggc ctttccgatg
      961 gcccggcctg ccgtggagcg cgacggcatc ctctatgcgc ctgccagctc catgtcgcac
     1021 ctgataccag ggctcacctt ctccgtgcct caggggtaac gccccgtata gaggagcggt
     1081 tctcagtggc agaccgcccg gcagccaccg cgcaaggggc gccgggcggt ctgcctcttt
     1141 tcctgcttcg gaccacgcgg gcaggcaggg tgcgacccaa cccgcgaggt aaagcccgga
     1201 agccgcccgg cagtcaagca gccagacgga aaattggatc cgctgtagca gcgaccctct
     1261 ccaactcagc cagctcgctc ttcgtgagtg gcttgaagcg ctccgtcacg gcattcaggg
     1321 ccatccggaa gaggcgcacg tcccccggcg gcaccgcggc cgtgatcggc tgtgagagcg
     1381 tgaaccgcag tgccatcgaa gccaggcggg gatcgtcgat cggctgatac cagcacttcg
     1441 ggtaggtgcg cggggtgcct ttcggcatcc ggccaagcgc catcgccttg attgccaggc
     1501 ggcccatccc cttcttctcg gcggccgcga tcacctgcgg cccgaacccc gccttgatcc
     1561 aggtgacgaa gttcacagga aagaggatcg tatcgaagga gaagcgctcc atcgcttcga
     1621 gcgccacctc cgccgaatga gcagagaagc cgatccagcg gaccagcccc tctttgcgcg
     1681 cctcggccac cgcctcgagc gcgccaccgg gcccagccgc cttctccaga tcgtccatcc
     1741 cgcggagacc atgcagctgg tagaggtcga agtggtcggt gcgtaggcgc tgcagcgact
     1801 gtcggagctc tgccgcggcg ccggcccgat cgcgcttctc cgttttgcac gccaggaaga
     1861 ccttatcgcg caatccctcg agcgccggcc cgaggcgctc ctcggcgttt ccatagctgg
     1921 gagccacatc gaagtagttg actccgcgct cgtaggcttc gacgacgatc cggcgggctt
     1981 catccggctc ggtgttcgcc acgacaatcc cacccaggcc gatgatcgac agctccacac
     2041 ccgtcctacc gaggcggcgc ctgggcaccc ggccatcctt ggccgcgatc ccatccaagc
     2101 cgagcgccgt agccgccaat gttcccgctc ctttgagaaa cgtgcgtcgg tccatctcac
     2161 ccctccacgg gccacgtcgg caggtccgcg ccccacggct ccgctcaagg cttcgttgag
     2221 ctcgctctct cccccgccct gccggatgcc acccgcgcgt ccgggccatc cccgatggct
     2281 gcgttcaccc gcgtggctgg ggcagatagg agcagtacgg ctcctcggca agaaagtcgc
     2341 ccgtggcgta gtacgcgcgg gcccggcagc caccgcaaac cgtgcggtac tcgcaggcac
     2401 cgcactttcc cccaagggcg tcttcgcgca atgtgcggaa gagaggcgat tcgcgccaga
     2461 tctccgcgaa atcgcgcgtt ctcacattgc ccgcctccac cgggaggtag ccgcagggga
     2521 agacctggcc cttatgcgag acaaagcaca ccgccgtgcc agccaggcag ccgcgcgtca
     2581 tcgcggccat cccctcgcgg gggccagacg gggcgatccc ctccgcgcgg gcgcgctggc
     2641 gagcgatgcg gtagtagtgc ggcgcgcacg tcgccttcag ctgtaactct ccgcgccgcg
     2701 acaggtcgta gaactcgttc agtatctcct catagcgcgc aggcgagagc atttgatcgt
     2761 cggcgatctg caccccacac ccgacgggta ccagcatgaa ggcatggaac gccacggcgc
     2821 ccaccgttcg cgccagctcg tagatgccgg ccagctgctc cgcgttgtgt cgggcgatgg
     2881 tggtgttgat ctgcacctcg acgccggcgt cgcgcaggta gcccagcccc cggagcgctg
     2941 cgtcgaacga gccggggagc gcccgaaagg tgtcgtgcgt cgccgcgtcc gcgccgtcga
     3001 tgctgatgct gacgcgccgg atccccgcat cccggacctg gccggcgact tgcggcgtga
     3061 tcagggtgcc gttggaagcc atcgagacga cgaggccctg ccctacggcg tggcgcgcca
     3121 cggtgaacag gtccgggcgc aagagcggct cgcctccaga gaggaccagg atcggcttgc
     3181 ctgcgcgcgc gatgctctcg accagggcaa gagcttcctc tgtagagagg tcgtcgcgca
     3241 tcctctcccg ggccacctcc aaccgccggc agtggatgca ctccaggtta catccggccg
     3301 tggtctccca aaagaccaag cgcagcctcg atccgcccgc gcctgccggc gcgtgacctg
     3361 gagcaccgct ataatggcct cccatccgta tccccttccc ccttcatcgc cgctggcatc
     3421 gcgaggggcg gaaccccgcg tgcgctcgct gtgggatggc ttagtctccg atggatgcga
     3481 cagtcaccgg cagccgcccg gggcagcggc gttcgccggc aagcgcctcg gcagccgcgg
     3541 tgagcgtgaa ggagtcggcc ccatagccga cgaccaccgg cacgcccgcg ggcaggtcgg
     3601 ccagggcata gggcgactcg aacagcatca tcgcgcggag cttgccggag gccgccacct
     3661 cgcgccacag ctcgatctgg gctccaggca ggcgcgtgcc ttcccccttg tagcagagga
     3721 tgtgcgcgaa agtcgcgccc acgaccgccg tgctggcagc cacggaagcg cgcactgctt
     3781 ccagatcccg cggtgtcggc acttcgtcga agacgagggt ctcgaagccc gccttctcgc
     3841 gcaggatgcg caccagcggg tgggtccccc gcaactcttg gccttcacgc tcctcggcaa
     3901 tgccaacacc ccggcgcttc agatcgtcgc acacgatcag cagaggcctc tcgcccaggt
     3961 cggggcgctc cgagagcgcg cccaccgccg tcacggaagc ctccgcgacc gcccgggcta
     4021 cggacgccag gtgggaccga atctcctccg ccgggggcag cggggcattc tcgcgcagcc
     4081 gggcctttag cgccgtgacc acggaggccg cggctcgcag gcgtgcctcg ggaacactgc
     4141 cgtcgcggac ggcttcaacc acggcatcga cactctcgct gggaggacgc ttgtagtcga
     4201 tgagaacctg atcatggccc gccaccaatg cccgccaggc cgcctccgtc gaatcgagcg
     4261 tggtgcgcag cccctccatg ccaagcgagt ccgtgaagat cggccctggg aagcccaggt
     4321 cgtcgcgcag gatgctaatc tgcgcagggc tgacagtaca gcagttctcc gggtcgagcg
     4381 agggcaccag caggtggccg gtcatcacgc tttcgaggcc tgcggcgcgc gccgtccggt
     4441 agggcagcca ctcgacggct tccaggcgct cgcgcgtcac gttgacggta ggcagcgaga
     4501 tgtggctatc cacggccacg tctccatgtc cgggccagtg cttagcgcac ggatggaggc
     4561 cgctctcttt catcccgcga gtgagcgctg cgccaaggcg agagacaagt ccggcatcct
     4621 cgccgaagga gcggacgttg atgatcgggt tatccgggtt ggtgttgaca tcaagcaccg
     4681 ggctccaggt gacgtcgatt ccgagccgcc ggccttccag gcccactgcg tagccccagg
     4741 cccgggccag ctcctcgctc ccggcagcac caagggccat cagcggcggg atctgcgtcc
     4801 cgtcgaaggc aaagccgctc cctgtctcca tatcacactg gagcagcatc ggcccgggcg
     4861 ccatttggcg caacgccgcg caccgggcgg cgatgtcggg gccgcccacc tgcgagttca
     4921 gcaccgcccc ggaggcgacg aggcggtctt cctcctcacg ccagttcggg gtgccccgaa
     4981 gcaccagcac ctgcgccacc aacgatcgtg cttcacgctc atccatcacg ctttctcccc
     5041 catccgaacg cgggtcgcat cggcccgcgc cataggagtc tacgccactc caggcgaacc
     5101 ttcctttgcc ccacagcacc actcctccct cccgacgcgc gttcatcaaa tcgccccatg
     5161 ccttgtaggg cattgcccct ataccaattt ccggtagaaa caccagttgc ccgttcggca
     5221 tgcgatgtgg tatgatccac gcgggttggc cgagtgaatg ggccgattgt ggaaaggtaa
     5281 acgcttcgct gccgagcgat tcccgcccgc tcgctcgtcc cttttcgctt agtgggagct
     5341 ggaaccgcgg gcctctcgcc gctgcgagag gacgcaaggt ttggcaggcg aaggccatga
     5401 catgggagac cgctctggtc tcctggagcc caggccccgc cagagcacac gcaatggatt
     5461 ccccgcgtgc gcactctcct ctctccatga gcagtctgaa ggcactctgg cctcaggcgc
     5521 ggctgcgtcc ggtcggtgtg tcattcccca tgtcactccc ctggcgctcg ccagggggct
     5581 tgggccaggt gtgagccgaa tcgttggacc gggggccgtc gtttctcccg actgctccat
     5641 tctaggacga ggtggcccgc gatacttcgg cggggggggc cccgccgctc gcccaaggca
     5701 gagcatgatg ctggagcagt tgaccgcgtg ggggtttcca cgccagccgg gcacttcaga
     5761 gccgtctgcc gccggtgagc aggcgaccct cctagacccc gcgtgcgcag gggatgatgg
     5821 cacgcgaggg aatcttgctt ccagcatccc cggatgatcg tgacagatga gataccgttt
     5881 gggaaccatg tcggtggaag aatgatggca cggaagccat gtctccgaat cgtcggcttg
     5941 tcgcggacgg aacgttcatt gctcgcgtct atcctccgcg ggcacggctt cgaggtaatg
     6001 gaggcggacg cgccgagctc ttgcgaggtg cctacgctcg agccgtgcaa catcctaacc
     6061 gctcgtcagg tgcaggtcct gcgcgccctc cggcgctatg gttcgcgaga gatgaccgcc
     6121 cgcgccctcg gcattgccct aaagacggtg gacgcccatc tggagcatat ctataagcga
     6181 gcaggtgtgc gcacacggat cgaggccctc tgctgggcgt gccggcgggg gttgctagat
     6241 gaagatcgga gcggttgcga agcgagcggt tcccggccga aagcctcacc agaagccctc
     6301 gcctaacctc cgcaccaccc attgaatgac actttcggtg cgccttccgc ctgttgacgg
     6361 caggcaccct cggtgataga atgtgagcag gcgcatggac cccagggact cgtggtgctt
     6421 cccagatcgt cggaagtcct tcctgccgct ggcgtcctgg tcaggaggct gccgtgagga
     6481 aagcccctat tggtcacagc ccgcgcattc aaatcgctta ccggtacggt gcaaccgcgc
     6541 tcgcagactc gtggcgccct gttcgatgca ctcgcacgcc ggggttcacc ctgatcgagc
     6601 tgctcgtggt gatcgccatc ctggctatcc tggctgcggt ccttctcccc gtcttcgcgc
     6661 gcgcccgcga aaaggctcgc gcggccacgt gcgcatccgc tcggcgcggc ccgaggacgc
     6721 gccgcgcatc cagcccaact acctcgccac cgaggacgac cgcaaggtgg cggccgacgc
     6781 gctgcgcctg acgcgccgca tcgtcgccat gccggcgctg gc
//
LOCUS       DAOSCV010000094         6805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7185_length_6805_cov_3.282074, whole genome shotgun sequence.
ACCESSION   DAOSCV010000094 DAOSCV010000000
VERSION     DAOSCV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7185_length_6805_cov_3.282074"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..621)
                     /locus_tag="PLY56_03610"
     CDS             complement(343..621)
                     /locus_tag="PLY56_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ20596.1"
                     /translation="MAADKTLACRDCGAEFVFTAGEQEFFAQKGFSDPTRCPACRAAR
                     KMERMRQQNEAMSAPMGGSFSSRGRDRDRRGGRDDEYDYDERPRRRRW"
     gene            912..1076
                     /locus_tag="PLY56_03615"
     CDS             912..1076
                     /locus_tag="PLY56_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20597.1"
                     /translation="MAAGITLYRLPEGSPACGDTGAPCLREARLDVNVGEKKPGQAWP
                     RSLLSLAGEE"
     gene            1081..2097
                     /locus_tag="PLY56_03620"
     CDS             1081..2097
                     /locus_tag="PLY56_03620"
                     /inference="COORDINATES: protein motif:HMM:NF020219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20598.1"
                     /translation="MALKVGFIGVGGIARRHLENARKREDIAMVAFCDVVPERARKCA
                     EEFNGNAYENFVELYDKEKPDAVIICTPPFAHGDIEEEAARRGIHFFVEKPVAVNMET
                     ANRIARIVEEAGIITQVGYMYRTSEAIKKVRELLSTRAIAMVQAHYYMPGLPSPGWWP
                     KMELGGGQLVEQATHMLDLGRFLAGEVAAVTGRTARVRDWTPPAGYQPGPGLLKYSDS
                     FEIPDTTALVLQYESGALGTLSCSLVPQKSWDVGFKVVADGLLVTIEGANARWVGDEE
                     GEMKAGPNYPAYVLYEFFDAVMAGKKTDIPYAEGVRSLAVSLAGYESVKRGGAPVALS
                     ELVG"
     gene            complement(2215..3180)
                     /locus_tag="PLY56_03625"
     CDS             complement(2215..3180)
                     /locus_tag="PLY56_03625"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ20599.1"
                     /translation="MRALQIQAPGRAEVVDIAMPQPGPGEVRVRVTLVNTCPQWDLHL
                     DAGEPMFVGQQISYPYTAGQPGHEMVGVVDAIGEDVTTFRVGQRVAAWRDAGHHRQGC
                     YAEFVNHLEANLLPVPDHVEDRAVASLELAMCVAVSILPLKRLGAIEGKRAAVNGLGP
                     AGMIAAQMLRAEGAASVTGFEPHPARRDLATGYAVDEALDPHDPATIERYPHRHCAEC
                     LEVGVDCAGYPDAVRFFMDRVRWHVALFAVQRHDYTYARTHSGLTVVGYAGHHRAAAE
                     YALEMIAAGKLDLRPLISVELPLERYAEGVALLRQQQALKIGFRP"
     gene            3728..4579
                     /locus_tag="PLY56_03630"
     CDS             3728..4579
                     /locus_tag="PLY56_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOJ20600.1"
                     /translation="MRQTQEVRRTRPPSGEAARVITICSGKGGVGKTSITLSLAYAMM
                     QRGARVLVLDADLGMANVEVLLGTSSAYNLLHVIRGMKSLRDVVVEGPRGLRWIPGGS
                     GVAELADLTADQLHSLVASFGELDATTDVLLIDTGAGISRTVTSFIQAADEAIVICTP
                     EPTSITDAYGVIKTVSAGERPIPLSLLVNRAVNDREAMDVGRGVQRAARRFLNRDVSV
                     LGYLPEDPLVARAVRAQQPFFLMQPSARVSRSVDQVAAKLIHQEQEGARPKGLGAFIK
                     RFASFFE"
     gene            4737..>6805
                     /locus_tag="PLY56_03635"
     CDS             4737..>6805
                     /locus_tag="PLY56_03635"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20601.1"
                     /translation="MRYLMLWILMLPLAVAAAAPEENAPAPEIHDLYIIPELRGETVD
                     LKVRWTTPTPTRGRVALADGNRVVEEDPSCLRGSTNARDRGEGWANNHRATIADVPGR
                     GPWRIRITAVDRQERVAERVATATLEQRAQKGQEGKVALRILGYAPEREGEVAATGIP
                     FPRGVLFDASQLRARAADGRLVPVQAEVWSRWPGDGSIKWVLAALPAPGPEVTLEYGV
                     PAVAPKAPLRVTETPDAITLDTGAARLVVPRREGVAGGFFRSQERRGGFPVSVLVDAE
                     SHRLQGRILRAVVENGPAPALRAVVRVDGEHAAQGQARPRYPFTLRIHACAGASGFRI
                     FHTFENDHIAQEMTSFQALELRFPGAVATTVVGERAHPLPAGARLFQQEDNRYVLTAP
                     ASGEGKHAAGWVNTAQGARIGVRHFREQYPKSIEAAPDATVIGLLPALPGPIYDGRPE
                     EPKLFAYLRGGRYTFRQGFAKTHELYIDVAGDARAQTRLDEPLVLAAPPEWYARGALR
                     ALGPLETEWAGYNAITARNLEMLLERREAERELGLMHFGDWFGERTWNWGNLEYDLHH
                     GLFLQFARTGDRRFFDQAVISARHQMDVDTIHHHREPARVGQQWIHSVMHTAGYYPKE
                     FQGMGRYAAEGWSDNRGHVWAGGLFDAALFTGDRRAWEVATRIADWAAGPQITNFDFG
                     NAREPGW"
BASE COUNT         1121 a         2196 c         2375 g         1113 t
ORIGIN      
        1 catcccggca ctccggccac ctcctgggcg gcgacgggaa ggcacgagca gagcgagaga
       61 ccgacaagca attgacccac acggcgcgtc cgcgcgccga actcccgaaa gcatgaaaga
      121 acgcccaacc gcagactcct cccaaagaga ttcaagagcg atttcaccgc gccagatgcg
      181 cgctcctctc tctgcgagca tcgccgggtc tgctgtgggt gattggcggc gcggacgaag
      241 tgccgcgctt ctgaggggaa ggggcgcggg gagggcgggg aagggggctt caaacgcgcg
      301 aagggagccc tgggggctcc cttcatcggt tggattcggc tgttaccagc gcctgcgccg
      361 tgggcgctcg tcgtagtcgt attcgtcatc gcggccgccg cgtcggtcgc ggtcacgccc
      421 gcggctgctg aagctgccac ccatcggtgc gctcattgcc tcgttctgct ggcgcatccg
      481 ctccatcttg cgtgcggcgc ggcaggctgg gcaccgcgtg ggatcgctga agcccttctg
      541 cgcgaagaac tcctgctcac cggcggtgaa cacgaactcc gcgccgcaat cgcggcaggc
      601 cagcgtctta tctgctgcca tctcctcccg acctcctttc gtaaaagcgt tcccggtgcg
      661 aggcactcgc ccgcatcgcc caacgtccgg agagcatggg aaaccgatgg cagccacact
      721 cttgcagggg ctattcgtca tcagagccac accggggtat aaactggcgc ctattatagc
      781 atgaatcgtg ccagatgaat acacccgcgg cgcggtctct ccggatctcg cgagcgcatc
      841 gagaaaccat ttccggatgg gcttcccgag ggctgacaca tccgccgggt gccggatgaa
      901 agaggagccg gatggctgca ggcataactc tgtaccgcct gccggagggg agtcctgcct
      961 gcggtgatac gggagccccc tgcctgcggg aagcgcgcct cgatgtcaac gtcggcgaga
     1021 agaagcccgg ccaggcatgg ccgcgttcac ttctctcgct cgctggggag gagtaatcgg
     1081 atggctctga aagttggttt catcggcgtc ggtggcattg cccgccggca cctggagaac
     1141 gcgcgcaagc gcgaggatat cgccatggtc gctttctgcg acgtggtgcc ggagcgcgcg
     1201 cgcaagtgcg ccgaggagtt caacggcaac gcctacgaga acttcgttga gctctacgat
     1261 aaagagaagc ccgatgccgt catcatctgc acgcccccgt tcgcccacgg tgatatcgag
     1321 gaggaggcgg cgcgccgcgg catccacttc ttcgtcgaaa agcccgtcgc cgtcaacatg
     1381 gagacggcga accgcatcgc ccggatcgtt gaggaggcgg ggatcatcac ccaggtcggc
     1441 tacatgtacc gcacctccga ggcgatcaag aaggtgcgcg agctcctatc cacgcgcgcc
     1501 atcgccatgg tgcaggcgca ttactacatg cccgggcttc cctctcccgg atggtggccg
     1561 aagatggagt tgggcggcgg gcagctcgtt gagcaggcaa cgcacatgct cgatctgggc
     1621 cgcttcctgg ccggcgaggt ggccgcggtc accgggcgca ccgcgcgcgt gcgcgattgg
     1681 acgccgccgg cgggctatca gcccggccct ggcctgctga agtactcgga ctccttcgag
     1741 atcccggata ccaccgccct ggttttgcag tatgaaagtg gcgctctcgg cacgctgagc
     1801 tgctcgctgg tgccgcagaa gtcctgggat gtcggcttca aagtggtggc ggatggcctg
     1861 ctggtcacca tcgaaggcgc caacgcgcgc tgggtggggg atgaagaggg cgagatgaag
     1921 gctgggccca actatcccgc ctacgtgctc tacgagttct tcgatgccgt catggccggc
     1981 aagaagacgg atatccccta cgcggaaggg gtgcgcagcc tcgccgtctc gctggccgga
     2041 tacgagtcgg tcaagcgcgg gggcgctcct gtcgccctat ccgagctcgt cggctgagaa
     2101 acgcgcagct cacatggagt gatcgggctt cgtcctgcca tcgaatggca gggcgaggcc
     2161 cgatcacgtg tcacgagcct cccgctccat tatgccgcca ggtgccccgt ggcctcacgg
     2221 ccggaagccg atcttcaggg cctgctgctg ccggaggagg gcgacgccct ccgcatagcg
     2281 ttcgaggggg agctccacgg agatgagcgg acggagatcc agcttgccgg cggcgatcat
     2341 ctccagggca tactccgcgg ccgcacggtg atgcccggcg tatcccacga ccgtgagccc
     2401 tgagtgcgtc cgggcgtagg tgtaatcgtg ccgctggacg gcgaagaggg ccacatgcca
     2461 gcgcacgcgg tccatgaaga agcggacggc atccgggtag ccggcgcaat cgaccccaac
     2521 ttccaggcac tccgcgcagt gccggtgggg ataacgctcg atggtggcgg gatcgtgggg
     2581 gtcgagggct tcatccacgg cgtagccggt ggccaggtcg cggcgtgcgg ggtgaggctc
     2641 gaagccggtc acggaggcgg cgccctcggc gcggagcatc tgggcggcga tcatcccggc
     2701 gggcccgagc ccgttgacgg cagcgcgctt accctcgatc gctcccaggc gcttgagagg
     2761 caagatcgag acagccacac acatcgccag ttccagcgag gcgacggcgc gatcctccac
     2821 atggtcgggc acgggcagga ggtttgcctc caggtggttg acgaactcgg cgtagcagcc
     2881 ctggcggtgg tgcccggcat cgcgccaggc agccacgcgc tggcccaccc ggaaggtggt
     2941 gacgtcctcg ccgatcgcat ccaccacgcc caccatctcg tggccgggct ggccggcggt
     3001 gtaggggtag gagatctgct gcccgacaaa catcggctcg ccggcatcga ggtggagatc
     3061 ccattgcggg caggtgttca ccagggtcac gcgcacccgc acctcgcctg gccccggttg
     3121 gggcatggcg atatcgacca cttcggcccg tcccggggcc tggatctgta gcgctctcat
     3181 gactctgctc cccctccagg agtggtgcgc tgctgcctcc acgccggcct ccgtgtgcca
     3241 aagaggccgg tcgcgacccg tggcaaggca cgctcacccc cagtgctttt cacgctctcg
     3301 ccaggcgatt ccttgggcct cagcgcggtt gtcccccggc catgggccgg catcgatacc
     3361 acggatgaag gtgacggaca cggatgggga ggtgcaggca cctggaacct gcacacgcgt
     3421 gggtgttaag ccggagaacg tactgcccgc acacggcatc acaccaaagc ccatcagctc
     3481 ttccactccc atttatctgt attcatcttc taacgctgcg tccgcgcgat ttgaggcagg
     3541 catccgctgg cgcgcgtcga actcccagaa gcggttgagg atcgccgctg ggaagtgagt
     3601 ggggctgtct aacctacctt cagagacggc aggtacgcct ttcgcgcggt gatctgggaa
     3661 gagccttggg cgtggctaga tggagacggc ttctgtgagg gaccaggcgg atagtctacg
     3721 caatctgatg cgccaaacgc aagaggtaag gcggacgcgt ccaccatccg gtgaagcggc
     3781 gcgggtaatc acgatctgca gcggtaaagg aggcgtgggg aagacgagta tcaccctgag
     3841 cctggcctac gcgatgatgc agcgtggcgc gcgcgtgctc gttctggacg ccgatcttgg
     3901 aatggcgaac gtggaagtcc ttctgggaac gagcagcgca tataacctgc tgcatgtcat
     3961 ccgcgggatg aagagcctgc gcgatgtggt ggtcgaaggc ccacgcgggc tgcggtggat
     4021 tcctggaggc tctggcgtcg ccgaactcgc cgatttgacc gcggatcagc tccactcgct
     4081 ggtcgcttcg tttggcgagc tggacgcgac gacggacgtc ctcctgatcg ataccggggc
     4141 gggcatctcg cgcacggtga cgagcttcat tcaggccgcc gatgaggcga ttgtcatctg
     4201 cacgccggag cccacctcga tcaccgatgc ctatggggtg atcaagacgg tgagcgccgg
     4261 ggaacggccg atccccctct ctctgctggt gaaccgcgcg gtgaacgacc gggaggcgat
     4321 ggacgtgggg cgaggcgtgc agcgcgccgc gcgccgcttc ctcaaccggg atgtcagcgt
     4381 gctgggctat ctgccggagg atccgctggt ggcgcgtgca gttcgggcgc agcagccgtt
     4441 cttcctgatg cagccctcgg cgcgtgtctc ccgctcggtg gaccaggtgg ccgccaagct
     4501 gatccaccag gagcaggagg gcgcgcgccc caaggggctg ggtgccttca tcaaacgttt
     4561 tgccagcttc ttcgagtgag aacacaccgc ggctccagga gctgccatcg atggcggcga
     4621 aggagggggc aaccgcgggc cgccgccctg aaggcatccg gagtcgtgcc ggggctgcct
     4681 cgtggcgagt ctttatcggc atcggcgggc gctctgcccg ggatgagagg taaacgatgc
     4741 gttatttgat gctctggatc ctgatgcttc ccctggctgt cgcagccgcg gcgcctgaag
     4801 aaaacgcgcc cgccccagag atccacgacc tctatatcat ccccgagctc cggggggaga
     4861 cggtcgatct gaaggtgcgc tggacgaccc ccacgccgac gcgcgggcgc gtggccctgg
     4921 cagatggcaa tcgtgtggta gaagaggatc cctcttgcct gcgtggctcg acgaacgcgc
     4981 gcgaccgggg cgagggatgg gccaacaacc atcgcgccac gatcgcagac gtgccgggtc
     5041 gggggccctg gcgcatccgc atcactgccg tcgaccgcca ggagcgcgtg gccgagcggg
     5101 ttgccaccgc cactctggag cagcgcgccc agaagggcca ggagggcaag gtcgcgctca
     5161 gaatcctcgg ttacgcgcca gagcgcgagg gtgaggtggc tgcgaccggt attcctttcc
     5221 cgagaggggt gctctttgat gcctcgcagc tgcgcgcgcg tgccgcggat gggcgtctgg
     5281 tgccggtgca ggccgaggtg tggagccggt ggccgggcga tggtagcatc aaatgggtgc
     5341 tggcagcgct gccggcgccg ggcccggagg tgaccctgga gtatggtgtg ccggccgtcg
     5401 ccccgaaagc cccgctgcgc gtgaccgaga cccccgacgc gatcaccctg gataccggag
     5461 cggcccggct ggtggtcccc cggcgcgagg gggtggcggg cgggttcttc cgcagccagg
     5521 agcgccgcgg cggcttcccc gtctccgtgc tggtggatgc cgagagccac cgcctgcaag
     5581 gccgcatcct ccgcgcggtg gtggaaaatg gtcccgcgcc ggcgctgcga gcggtcgtcc
     5641 gggtggatgg cgagcacgcc gcccagggac aggcaaggcc gcgctacccg ttcacactgc
     5701 gaatccacgc gtgtgctggc gcctctgggt tccgcatctt ccacaccttc gagaacgacc
     5761 atatcgcgca ggagatgacg tcgttccagg cgctggagct gcgcttccca ggcgcggtgg
     5821 cgacgactgt tgtgggcgag cgcgcgcacc cgctgccggc gggcgcccgg cttttccagc
     5881 aggaggataa ccgctacgtg ctcacggcac ccgcctccgg cgaagggaag cacgcggccg
     5941 ggtgggtgaa cacggcgcaa ggcgcgcgaa tcggggtgcg ccacttccgc gagcagtatc
     6001 cgaagagcat cgaggccgcg cccgacgcca ccgtgatcgg cctgctcccg gcactgcccg
     6061 ggccgatcta cgatggccgg cccgaggagc cgaagctgtt cgcctacctg cgcggcgggc
     6121 gctacacctt tcgccagggc tttgcaaaaa cgcacgagct gtatatcgac gtggcgggag
     6181 acgcgcgtgc ccagacgcgc ctggacgagc cgctggtcct ggccgcgcct ccggagtggt
     6241 atgcccgtgg cgccctgcgc gcgttgggcc cgctggagac ggagtgggcc ggctacaatg
     6301 cgatcaccgc gcgcaacctg gagatgttgc tcgagcgccg agaggccgag cgcgagctgg
     6361 gcctcatgca ctttggcgac tggttcggcg agcgcacctg gaactggggc aacctggagt
     6421 atgacctcca tcacggtctc ttcctgcagt ttgcgcgcac gggtgaccgg cgcttcttcg
     6481 accaggcggt gatcagcgcg cgccatcaga tggatgtgga tacgatccat catcaccgcg
     6541 agccagcgcg cgtcggccag cagtggatcc actcggtgat gcataccgcc ggctactacc
     6601 cgaaggagtt ccaggggatg ggccggtatg ccgcggaggg gtggtcggac aatcgcggcc
     6661 acgtctgggc cgggggcctc ttcgacgcgg cgctcttcac cggcgaccgg cgcgcctggg
     6721 aggtggcgac gcgcatcgct gattgggcgg cgggcccgca gatcaccaac ttcgacttcg
     6781 gcaacgcgcg cgagcccggg tggat
//
LOCUS       DAOSCV010000095         6801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7195_length_6801_cov_3.564334, whole genome shotgun sequence.
ACCESSION   DAOSCV010000095 DAOSCV010000000
VERSION     DAOSCV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7195_length_6801_cov_3.564334"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLY56_03640"
     CDS             complement(<1..210)
                     /locus_tag="PLY56_03640"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HOJ20602.1"
                     /translation="MPIYTRGGDAGETDLGTGLRVPKECARVEAYGTVDELNAHLGVA
                     RATVGDSDLAALLARVQQELFLIGAN"
     gene            600..1235
                     /locus_tag="PLY56_03645"
     CDS             600..1235
                     /locus_tag="PLY56_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ20603.1"
                     /translation="MIFCRYLLAIALAMAVSGRTSAAQPEQLPERTLLPGEVIRIEIE
                     GEEELSRTVTIGSDGKVSLGFPGEVELAGLTRPEAAEAIRQTLLRYLRDPVVAVSLVE
                     HRFSVLGAVRRPGQFPMLGDQIPVLDALAQAGGPEDRANLRKVRLVRQTREGPHKVLL
                     DLQSAIREPGSYALPLMQPGDVLYVDRRKPALGTTLLTVAGVIASMAWAFR"
     gene            complement(1304..2614)
                     /locus_tag="PLY56_03650"
     CDS             complement(1304..2614)
                     /locus_tag="PLY56_03650"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20604.1"
                     /translation="MKCNTRRSAAAPLFATTLALLSYLGLSHPGPALAYGTKAKGLAG
                     GGVALSQDATAARLNPAGMVFAGTRADTGLALMTSERAYSVSGQPSGAPNTFPLFPES
                     VENEDSLALVPSAAINWMLDDRSAIGLTTYGHGVMYTRYPETAGGGTGTYGAGAAGIR
                     TEQFYVLPTYARKFGEGSAWGVSAVMVYHRFRAQGMGSMGSYVSDGVPDNLSDNGTDT
                     AFGIGAKFGVQSRVTPTLTLGASYQTKVKMSRLKKYADLFPDGGRFDAAPVLQVGAAW
                     AVSPSLTAIFDIEHTDNSAVPALANPFSKFLQAATAGNPDGLLGGRDGAGFGWRDVTT
                     YKLGLQWEASPGWTWRAGASYGRQPIPTSEVAMNILGANIAEWRVTAGLTRRLCDRHE
                     LNLSLMYSPRNKISGPNPLEAPGQQSIELSARDFEIETSYAWKF"
     gene            complement(2802..3425)
                     /locus_tag="PLY56_03655"
     CDS             complement(2802..3425)
                     /locus_tag="PLY56_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ20605.1"
                     /translation="MDELDEASRRALLARGVVRTYARGEVLFDEGEPGKGLFLIESGS
                     IKIYKVSETGREQILAVLRAGDSVAELPLFDRAPYPASAAALDQATVLFIPLRSFDEV
                     LAENPSIALAIIRALARRMRALVELIADLSLRQVRQRLARFLLEEAGESREVRLSLTN
                     EELAARLGTVRDVISRTLSSLQNDGLIQVEGRLIRLSDYDGLRQEAG"
     gene            complement(3627..5273)
                     /gene="hcp"
                     /locus_tag="PLY56_03660"
                     /gene_synonym="priS"
     CDS             complement(3627..5273)
                     /gene="hcp"
                     /locus_tag="PLY56_03660"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ20606.1"
                     /translation="MFCYQCEQTVKGTGCKTFGNCGKDPETAALQDLLVHGIQGLSMY
                     AHRARQLGVVDRALDTLVLEVLFTTVTNVNFDPQRLAAMARQVASERNRAKALYEEAY
                     AKQHGKKPEPLGGPAAWIPAETHEGLVRQGQDVGILPLVAKHGADVNGMLQLILYGLK
                     GMAAYADHAMILGKEDDAVYAFFHEALSYTASDTFKMDELIAMALKVGEVNLKVMGLL
                     DAANTGAYGHPTPTSVRVTPVKGKAILVSGHDLKDLALLLEQTKNAGVNIYTHGEMLP
                     AHGYPELKKYPHLVGNYGGAWQDQRAEFDAFPGAILMTTNCIQKPRDSYKDRIFTSGL
                     VAWPGVTHIADKDFTPVIEAALAAEGFTEDAEPKSITVGFGHNAVLGVAGQVIDAVKS
                     GAIRHFFLVGGCDGAKSGRDYYTQFAEQVPNDCVILTLACGKYRFNKQEFGDIGGIPR
                     LLDVGQCNDAYSAIKIASALAEAFGVGVNELPLSLVLSWYEQKAVCILLTLLHLGIKK
                     IRLGPSLPAFLTPAILDVLVKNFEIMPTGTPEGDLKAILG"
     gene            complement(5608..>6801)
                     /locus_tag="PLY56_03665"
     CDS             complement(5608..>6801)
                     /locus_tag="PLY56_03665"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOJ20607.1"
                     /translation="PQRMLSIIQEMAEIGSGECSAIPTAGLPHLIGGRVAYDATPEYF
                     ARYGRQMVEAGARIVGGCCGTTPAHIRALAQAVRGVEPGSRRVAVSVRTRSDETKATR
                     QAQASRLAEKLGRKFVVTVELDVPRGLDMSSLIQAAEALRDQGCDCINISDGARARLR
                     MNPMVVGRLVQEIVGIEVIQHFACRDRNLLAIQADLLGAHALGLRNILAITGDPTQVG
                     DYPTATSVFDVDAIGLVRILRAFNNGVDLSGTSIGEKTAFLIAVAYNPLARNPEEEAD
                     RLRRKADEGAHFIFTQPIYEMSVLEIAVREAERVGLPLLMGVLPLRSSRHAEFFHNEV
                     PGIQIPEEMRRALAELEGDDAKRYGIEQAQQFVRRAKGMTAGMYIMPPALSPHVAGQV
                     MEALH"
BASE COUNT         1131 a         2170 c         2324 g         1176 t
ORIGIN      
        1 attggcgccg atcaggaaga gctcttgttg cacccgcgcg aggagagccg cgagatcgct
       61 atcccccacg gtagcgcggg ccacgcccag atgggcattc agctcgtcga ccgtgccgta
      121 ggcctccacg cgggcgcact ccttgggcac gcgtaggccg gtgcccaggt cggtttcacc
      181 ggcatcgcca ccgcgggtgt agataggcat cgatactccc ttcgcgcggc gggcggaaga
      241 gggggccggc ccgtccaggc ggggaaccgg tgcgcgttgg tgggaatgca gagccggggc
      301 ccggccgcgt cttcgagctg acaaccactt ccggccgcaa tggatcttcg tgaacggcga
      361 tggacgcgcc gtcgccctcc acatgctcgc gattcgtgcc cgccgggttg ctctcctgcc
      421 gggcgggtag ccccacggcg catcggcgcc ggggaatgac aggcccggtg ggcctggccg
      481 gaccggcacg cgaattgccg ggacggtgcc cgtgagggcg ccatggactg cgccgtgcgg
      541 accgcgtgtg cctggcgcca ggcgttgccg tgccgcggca cgcccgggag gagttcacgt
      601 tgatcttctg ccgatacctg ctggccatcg cgctggcgat ggcggtaagc ggacgaactt
      661 cggcggcgca acctgagcag cttccagagc gcactctcct gcctggcgag gtgatccgca
      721 tcgagatcga gggcgaagag gagctctcgc gcaccgtgac gatcggatcc gatggcaagg
      781 tctcgttggg cttccccggc gaggtagagc tcgccggtct gacgcgaccg gaggcggcag
      841 aggcgatccg ccagacgctc ctgcgctacc tgcgtgatcc cgttgtcgcg gtctcccttg
      901 tggagcatcg cttctccgtg ctcggggcgg tgcgccgtcc agggcagttt ccaatgctcg
      961 gtgaccagat accggtactg gacgcgctgg cccaggcggg cgggccggag gatcgggcga
     1021 acctgcggaa agtccgactg gttcgccaga cgcgcgaggg cccccacaaa gtgcttctgg
     1081 acctccagag cgcaattcga gagccgggaa gctacgcgct gccgctgatg cagccggggg
     1141 atgtgctgta tgtggaccgc cggaagcctg ctttgggaac tactctcctg acggtggcgg
     1201 gggtgattgc atcgatggca tgggcgttcc gttaagccgc tcaaggctca aggccggcgt
     1261 gttgtggcga ccttgagcct tgaactccga acgccgcgcg ttctcagaac ttccaggcgt
     1321 aactcgtctc gatctcgaaa tcccgggcgg agagctcgat gctctgctgg cctggcgcct
     1381 ccaatggatt ggggccgctg atcttgttgc ggggggagta catcagcgag agattcagtt
     1441 cgtggcggtc gcagagacga cgggtgaggc ccgccgtcac gcgccactcg gcgatgttcg
     1501 ccccgagaat gttcatcgcc acctcggagg tggggatggg ctggcgcccg tagctggcac
     1561 cggcgcgcca ggtccacccg ggcgaggcct cccactggag accgagcttg taggtggtga
     1621 cgtcgcgcca gccaaacccg gcgccatcgc ggccgcccag cagcccgtcg ggattgccgg
     1681 cggtagccgc ttgaaggaat ttggagaagg ggttggcgag tgccggcacg gcgctgttat
     1741 cggtgtgctc gatatcgaag atggcggtga gagagggcga gaccgcccat gcggccccga
     1801 cctgcagaac cggcgcggcg tcgaagcggc cgccgtccgg gaaaagatcg gcatacttct
     1861 tcagccggct catcttaact ttggtctgat aggacgcgcc gagcgtcagc gtgggggtca
     1921 cccggctctg cacgccgaac ttggcgccga ttccaaacgc cgtgtccgtc ccgttatccg
     1981 atagattgtc aggaacgcca tcgctgacgt agctgcccat gctccccata ccctgggcac
     2041 ggaagcggtg gtagaccatc accgcggaga cgccccaggc ggatccttcg ccgaacttgc
     2101 gcgcgtaggt ggggaggacg tagaactgct cggtacggat gcctgccgcg ccagcgccgt
     2161 aagttccggt gccgcctccg gcggtctccg ggtagcgggt gtacatcacc ccgtggccgt
     2221 acgtcgtgag cccaatcgca ctccgatcat cgagcatcca gttgatcgct gcgctcggca
     2281 ccagtgcgag cgagtcctcg ttctccacac tctccgggaa gagggggaag gtgttcggtg
     2341 cccccgacgg ctgtccactg acggagtagg cgcgctccga ggtcatcagc gccaggcccg
     2401 tgtcagcgcg cgtgccggcg aagaccatgc ccgccgggtt gaggcgcgcg gcggtcgcat
     2461 cctgggacag ggcgacgccc ccgccggcca ggcccttggc tttcgtgccg taggcaagcg
     2521 ccggccctgg gtgcgagagg ccgaggtacg aaaggagagc gagcgtcgtt gcgaagagtg
     2581 gcgctgccgc agagcgccgg gtgttgcatt tcatggctgt tcatccttac gggagggctg
     2641 catccggatt ggaaacgtgc gcctgcacgc gggtgcagcg cgagcaagcg gcccgcctgg
     2701 cggccgaccg ctaagatcat tatcggctgg agagggagga acttaagacc gccggaaccg
     2761 gcgtggaatg ggaacgacgc tgcgatctca ggccgagtct gctacccggc ctcctggcgc
     2821 aggccgtcgt aatcggagag gcggatgagc cggccttcca cctggatgag gccatcgttc
     2881 tgaaggctgc tcagggtccg tgaaatcacg tctcgcacgg tgcccagacg cgccgccagc
     2941 tcctcgttgg tcagcgagag tcggacttcg cggctctcgc cggcctcttc cagaaggaag
     3001 cgcgcgaggc gctggcgcac ctggcgcagc gagagatcgg cgatcagctc cacgagggcg
     3061 cgcatgcgcc ttgcgagggc gcgaatgatc gccagggcga tggatgggtt ttcggcgagt
     3121 acctcgtcga agctgcgcag gggaatgaag aggacagtgg cctggtcgag cgcggccgcc
     3181 gatgccgggt agggggcgcg gtcgaaaagc ggcagctcgg cgacggaatc cccggcgcgc
     3241 agtacggcga ggatctgctc gcgcccggtc tccgagacct tatagatctt gatgctcccg
     3301 ctctcgatga ggaagaggcc cttgcctggc tcaccctcgt cgaagagcac ctccccccgg
     3361 gcgtaagtgc ggaccacgcc gcgcgcaagg agcgcgcggc ggctggcctc gtccagctca
     3421 tccaagaggg gataattgcg gctgagttcg cacggatttg tctgtcgggc tgccatggcc
     3481 gtagtatagc ggggggcacc cccgtgcgtc aaggcccgcg ccggcaaggt tgatactgcg
     3541 gatgagggat gaggcgcgag cacgccccat cccccatcag atcggtcacc tgcgggcaag
     3601 cgaagccggc ccgcccgcgg cggcgattag ccgaggatcg ccttcagatc tccttcggga
     3661 gtgcccgtcg gcatgatctc gaagttcttc accagcacgt ccagaatggc cggggtgagg
     3721 aacgcgggca gcgagggacc caggcggatc ttcttgatac ccaggtgaag cagcgtcagc
     3781 aggatgcaga ccgccttctg ctcgtaccag gagaggacca gcgagagcgg cagctcgttg
     3841 acgccaacgc cgaacgcctc cgccagagcg gacgcgatct tgatcgccga gtaggcgtcg
     3901 ttgcactggc cgacatccag caggcgcggg atgccgccga tatcgccgaa ctcctgctta
     3961 ttgaagcggt acttgccaca cgccagcgtg agaatcacgc aatcgttcgg aacctgctcg
     4021 gcgaactgcg tgtagtaatc gcgcccactc ttggcgccat cgcagccgcc caccaggaag
     4081 aagtggcgaa tggcgccgct tttcaccgcg tcgatcacct ggcccgcgac gcccagcacg
     4141 gcgttgtgcc cgaagccgac ggtgatgctc tttggctccg catcctcggt gaagccctcg
     4201 gcagcgagag cggcctcgat gaccggagtg aaatccttgt cggcgatgtg ggtgacgccc
     4261 ggccaggcga ccagaccgct ggtgaagatg cggtccttgt aggaatcgcg cggcttctgg
     4321 atgcagttgg tcgtcatcag gatggcgccc gggaaggcat cgaactcggc acgctgatcc
     4381 tgccaggcgc cgccatagtt ccccaccagg tgggggtact tcttcagctc cgggtagccg
     4441 tgtgccggga gcatctcgcc gtgggtatag atgttcaccc cggcgttctt cgtctgctcc
     4501 aggaggagcg ccaaatcctt cagatcgtgc ccggaaacga ggatcgcctt ccccttgacc
     4561 ggcgtcacgc gcaccgaggt cggcgtcgga tgcccgtagg cgccggtgtt cgcggcatcg
     4621 aggaggccca tcaccttcag attcacttcg cccaccttca aggccatggc gatcagttcg
     4681 tccatcttga aggtgtcgct cgcggtgtag ctgagcgcct cgtggaagaa ggcatagacg
     4741 gcatcatcct ctttcccgag gatcatcgcg tgatcggcgt aggcggccat gcccttcagg
     4801 ccatagagga tgagttggag catgccgttc acgtctgctc cgtgctttgc caccaacggg
     4861 agaatgccca catcctggcc ctggcgaacc aggccctcgt gtgtctctgc cggaatccag
     4921 gcggcaggac cgccgagcgg ctctggcttc ttgccatgct gcttcgcgta ggcctcctca
     4981 tagagcgcct tggcgcggtt gcgctcgctg gccacctgcc gggccatcgc cgcgaggcgc
     5041 tgcggatcga agttcacgtt ggtgaccgtg gtgaagagca cctccaggac gagggtatcg
     5101 agggcgcggt cgacgacgcc gagttggcgt gcccggtggg catacatcga aagcccctga
     5161 atgccgtgga ccagaaggtc ctgaagcgcc gcagtttccg ggtccttccc gcagtttcca
     5221 aaggtcttgc acccggtgcc cttgaccgtt tgctcgcact gatagcagaa catgctcatg
     5281 ctggtctcct tccttccgcg atcctcgtcg cgtcaacggc gtcattgtag cagcaacgcg
     5341 gggtgcgtct ctatgactta ggtcataacc ggacgtgtct attgcgagct tctgaaggaa
     5401 agcgcaacaa aggaagggac tgaagggcag agggctgccc gcgagccact gcccatcccg
     5461 cctgcctaac cggcttcgcg aaccaggagc gcccgcgaag ccggtctctc gatgagaaat
     5521 aggagttggc gcctcagcaa aagtacgcga gcaaccgtct gttcagttgc gcacgctgca
     5581 agcggttcac tcaaccactc cgccagatta gtggagggcc tccatcactt gaccggcgac
     5641 gtgggggctg agcgcgggtg gcatgatgta catgcccgcc gtcatcccct tcgcccggcg
     5701 gacgaactgc tgtgcctgct cgatgccata ccgcttggcg tcgtctccct ccagctctgc
     5761 cagcgcccgg cgcatctcct cggggatctg gatgccgggc acctcgttat ggaagaactc
     5821 ggcatgccgg ctgctgcgca gtggaagcac gcccatcagc agcggaagcc ccacccgctc
     5881 cgcctcgcgg acggcgatct ccagcacgct catctcgtag atcggctggg tgaagatgaa
     5941 gtgcgccccc tcatccgcct tgcggcgcag ccggtcggcc tcctcttcgg ggttgcgtgc
     6001 cagggggttg taggcgacgg cgatgagaaa cgcggttttc tcgccgatgg atgtgcctga
     6061 tagatcgacg ccgttgttga aggcgcgcag gatgcgcacc agtccgatgg catccacgtc
     6121 aaaaacggaa gtggccgttg gatagtcgcc gacctgagtg ggatcgccgg tgatggcgag
     6181 gatgttgcgc aggccgagag cgtgcgcccc caggaggtcg gcctgaatag cgaggaggtt
     6241 gcggtcgcga caggcaaagt gctggatcac ctcgatgccg acgatctctt gcaccaggcg
     6301 gcccaccacc atcggattca tgcgcagccg ggcgcgcgcg ccatcggaga tgttgatgca
     6361 atcgcacccc tggtcgcgga gcgcctcggc ggcctggatg agggaagaca tgtcgaggcc
     6421 gcggggcacg tcgagctcca cggtcaccac aaacttgcgc ccgagctttt cggccaagcg
     6481 cgatgcctgt gcctggcgcg tggccttggt ttcgtccgag cgcgttcgta cgctcacggc
     6541 aacgcgccgc gagccgggct ccacgccgcg cacggcctgg gcgagcgccc ggatatgcgc
     6601 cggtgtggtg ccgcagcatc cccctacgat ccgcgcgccg gcttccacca tctgccggcc
     6661 ataacgcgcg aaatactctg gcgtggcgtc gtaggcgacg cgcccgccga tgaggtgggg
     6721 aagaccggcc gtcgggatgg ccgagcactc tccgctcccg atttcggcca tctcctggat
     6781 gatgctgagc atgcgctggg g
//
LOCUS       DAOSCV010000096         6778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7228_length_6778_cov_3.880857, whole genome shotgun sequence.
ACCESSION   DAOSCV010000096 DAOSCV010000000
VERSION     DAOSCV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6778
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_6778_cov_3.880857"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLY56_03670"
     CDS             complement(<1..478)
                     /locus_tag="PLY56_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ20608.1"
                     /translation="MPTYRYRAKDRAGKTVTGCLTAEDQHATAKILRDMGLWPMEIEL
                     TGAASPVRVKQTGAATAIQGRPATASRAWSAPGVPLKDLAIYFRQLAGAVHSGMPLGR
                     CMEIMRDENRGTLKRVSAEAAEAIHHGQPLSVTFANHPRLFSPLILATIRAGEVGGR"
     gene            complement(563..1729)
                     /locus_tag="PLY56_03675"
     CDS             complement(563..1729)
                     /locus_tag="PLY56_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ20609.1"
                     /translation="MKQYERNLWVLSLAIFLASISWFQIMPFLPLFLEEMGVKENILQ
                     WSGLIFAIQSMASIVALPYWGKLGDRYGRKAMTLRAGFCLAGIYFGMSVCRTPYELAF
                     LRFLNGALTGFIPGAMALIATGTPQKEGPRAVAIAQSASAAGQIVGPGIGGFMASLFG
                     YRGSMSVSGSAVLFSTLLVLVMVREGPREVSEEKTSILDDFRLMLQSPVLASVMLVVL
                     VNSFFMSAVNPVLALHLREIGRGAPAWLAGCIFSLPATAFVLFAQRWTGYGDRAGYDR
                     AILTGLAGATLCGISLSLVRGIWVFALFFFALGVCVASIAPSAGAIICLRVPEHFRGR
                     AYGMQQSAVMVGALLAPITATQVAARLGIPAVFAINALLYLAGIAGFRALVRRW"
     gene            complement(2052..2741)
                     /locus_tag="PLY56_03680"
     CDS             complement(2052..2741)
                     /locus_tag="PLY56_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20610.1"
                     /translation="MEIITNCQRRYYPQFRNGTIRDIVQNRIGDLYFVRASTRGNTMG
                     MGPHMMDLLLFFLGEVEPEAVWAMAHTINEEGYQATHQAPESILAEYWFPNNVRVIFD
                     CDPEALGTPGEDSFWMHLHFDFLGSKGRLYLTQNKGYWYQSEGMAEPVHGESSFSNQE
                     AAGQRDFTQAVGDWLEHGTPHLNRFELHQRAVRALLAAQKSVYEGKRISLPTEFTDAE
                     WSALRERLRGT"
     gene            complement(2805..3092)
                     /locus_tag="PLY56_03685"
     CDS             complement(2805..3092)
                     /locus_tag="PLY56_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20611.1"
                     /translation="MATYRSLFLGCGARAQWHAPAYRELRNMELAGACDLDAGRLAAF
                     CQKYGVPAAYDDYEKALSDLRPDIVHVITCPGRRVWEIETAARAGVRAVIV"
     gene            complement(3172..4422)
                     /locus_tag="PLY56_03690"
     CDS             complement(3172..4422)
                     /locus_tag="PLY56_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOJ20612.1"
                     /translation="MMSAAISAVTLPASVHHTPRVHAACRTDEYLYHQLIPYIGNKRK
                     LLPLLGEALKLAGIGEGTFLDLFAGSGVVSRLAKQLGYRVLSNDWEPYAYVLNVAYVE
                     CNHPPQFRRWGGADALFHYLNALPPVDGYIATHYCPEDDERPDPDRERLFFTRRNGRR
                     IDAIREQVAAWEREGLLDERERAFLLAPLVYSASYVSNTSGLFKGFHRGWGGATRTAL
                     YRILSDIRLSPPLTLDNGEENLVFREDAGRLAEHLRADIAYLDPPYNQHPYGSNYHLL
                     NTLVLWDKPPVSRRIDEGIGGKSGIRTDWRTERRSAFNYRHQAADALATIISRLRVRR
                     ILMSYSTEGILSVEQVMEILGSRGPLQVLCQPYKRYRVSSQRYSPRPTTIEYVVVVDC
                     ERRNGGVGACQAAEQVRALAAETA"
     gene            4590..5636
                     /locus_tag="PLY56_03695"
     CDS             4590..5636
                     /locus_tag="PLY56_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ20613.1"
                     /translation="MRHVARHWRLPAFAAALALAFLAGRFATPGGPSQVHAEPSVSHG
                     QEAIIAVARAVSPTVVGVIRMQDGEREGLGSGVIVGEDGLILTNNHVVAGARELVVAL
                     ADGRELPATVVNTDPISELALVKVDAKGLPTATLGDSDQLRVAQTAIAIGNPLGFERT
                     VTVGVVSAINRHLPDRRAELDNLIQTDAAINPGNSGGPLVDIQGRVIGINTLVVRGPV
                     GAGGLGFAIPASTARDFISDVQRYGRVVRTWLGITPVDIDREIARRFDLPITEGVLIR
                     RVFSDSPASRAGLRERDIVVNANGTVVRNLGDLRRVIRSKRVNDPMTLTILRDGERIR
                     VMLRLAERPPDAGR"
     gene            6353..>6778
                     /locus_tag="PLY56_03700"
     CDS             6353..>6778
                     /locus_tag="PLY56_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ20614.1"
                     /translation="MASGSVKGLLGPRGRLAERIRGFEFRREQLEMAEAIAASLAEGS
                     HVLAEAGTGVGKTFAYLVPALLYLRHGRKVVLSTHTINLQEQLLEKDVPLLRDALPDR
                     SFRVALLKGMGNYLCLQNMDGVARLDLLEHEEWQRLRLWA"
BASE COUNT         1197 a         2123 c         2297 g         1161 t
ORIGIN      
        1 gccggcctcc cacctccccg gcgcggatcg tcgccaggat aagcggcgag aagaggcgcg
       61 ggtgattagc gaaagtgacc gagagcggct ggccatgatg gatcgcctca gcggcctcgg
      121 cggagacccg cttcagcgtg ccccggttct catcgcgcat gatctccatg caccgcccca
      181 ggggcatccc ggagtgaacc gcgccggcga gctgacggaa gtagatggcg agatccttca
      241 acggcacgcc gggcgcgctc cacgcgcggc tcgctgtcgc ggggcggcct tgaatggcgg
      301 tcgcggcacc ggtctgcttg acccggacgg gcgacgcggc accggtcaac tcgatctcca
      361 tcggccagag gcccatgtcg cggagaatct tcgcggtggc gtgctggtcc tccgcggtga
      421 ggcagccggt cactgttttt cccgcccgat ccttggcgcg gtaacggtag gtgggcatct
      481 gtgcgcctcc cgctctcttg tgttaaccta atgatacctt agcccgggag cacgcgtcaa
      541 tgtgcgcggc ggagtcgctc catcaccagc gccggaccag ggcgcggaac ccggcgatgc
      601 ccgcgagata gagcagggcg ttgatggcga agaccgcggg gatgcccagg cgcgcggcga
      661 cctgggtggc cgtgatcggg gcgagcaacg caccgaccat gacggccgat tgctgcatgc
      721 cataggcgcg cccacggaag tgctccggca cgcgcaggca gatgatcgcc cccgccgagg
      781 gcgcgatcga tgccacgcac acgcccagag cgaagaagaa gagggcgaag acccagatcc
      841 cccgtacgag cgaaaggctg atcccgcaga gcgtggcgcc agcgagaccg gtgaggatgg
      901 cacggtcata cccagcccgg tcaccgtagc cggtccagcg ttgcgcgaaa aggacgaacg
      961 ccgtggcggg gagcgagaag atgcagccgg cgagccacgc gggcgcgccc cggccaatct
     1021 cgcggaggtg aagtgccagc accgggttga cggccgacat gaagaagctg ttgacgagga
     1081 ctacgagcat gacggatgcg agcacgggcg attggagcat cagccggaag tcatcaagga
     1141 tgctggtctt ctcctcggat acctcgcgcg ggccttcacg gaccattacc agcaccagga
     1201 gtgtggagaa gaggacggcg gatcccgaca ccgacatcga gccccggtag ccgaagaggc
     1261 tggccatgaa gcctccgatg ccggggccca cgatctggcc cgcggcgctg gcgctctgag
     1321 cgatagcgac ggctctgggc ccctccttct gcggggtgcc ggtggcgatg agcgccatgg
     1381 cgccagggat gaacccggtg agcgcgccgt tgaggaagcg gagaaacgcc agctcgtagg
     1441 gagtgcggca aacgctcatg ccaaagtaga tgccggccag gcaaaacccg gcgcgcagcg
     1501 tcatcgcttt ccggccatac cggtcaccga gcttccccca atagggaagc gccacgatgc
     1561 tcgccatcga ctggatggcg aagatgagcc cggaccactg caggatgttt tccttgacgc
     1621 ccatctcctc caggaagagc gggaggaagg gcatgatctg aaaccagctg atcgacgcga
     1681 gaaagatcgc cagactgaga acccacagat ttcgttcgta ttgtttcatc aatcgacata
     1741 tttctaagcg ggatggctgg caaaacgagt agcagcggaa cgcgcgacag gtggctccct
     1801 ttcagtcgcg cattccgatt gggccaaagc gggtagatgc gaacggaggc ccgtcgcacg
     1861 ccgccccgtg ttgaacccgg aatctcatcg gatggaaccc gcccctattc taccacattc
     1921 taccatgagg atgcccattc cgatgagtgg ctgtcaggtc ccaggcgggc ggaggcgacg
     1981 ggcgagagtg gggtgtcctg cgcgcgccgg ttcggctccc tgcactgtga taggcgattt
     2041 gcaggcccgt cctacgttcc ccggaggcgc tcacggagcg cgctccactc ggcatcggtg
     2101 aactctgtcg gcagagagat tctcttcccc tcatagaccg acttctgtgc ggcgagcagg
     2161 gcgcgcacgg cgcgctggtg cagctcgaag cggttgaggt gcggcgtgcc atgctccagc
     2221 caatcgccga cggcctgagt gaaatcgcgc tggccggccg cctcctggtt gctgaagctg
     2281 ctctcgccat gcaccggctc ggccatcccc tcgctctgat accagtagcc cttgttttgg
     2341 gtgaggtaga gccggccctt gcttcccagg aagtcgaaat ggaggtgcat ccagaaggag
     2401 tcctctcccg gggtgcccaa cgcctctggg tcgcagtcga agatcacccg gacgttgttc
     2461 gggaaccagt actcggcgag aatgctttcc ggggcctgat gggtggcctg atagccctcc
     2521 tcgttgatcg tgtgcgccat ggcccacacc gcctcgggct ccacctcgcc caggaagaag
     2581 aggagcaggt ccatcatgtg cgggcccatg cccattgtgt tcccgcgggt gctggcgcgc
     2641 acgaagtaga ggtccccgat ccggttctgc acgatgtctc ggatcgtgcc gttgcggaac
     2701 tgcgggtagt agcggcgctg gcagttggtg atgatctcca tgtcatactg cgcgcggatc
     2761 cggctcagct cctggatctc ggagggcttg atcgccagtg gctactagac gatcaccgcc
     2821 ctcacgccgg cgcgcgccgc ggtctcgatc tcccagacgc gcctccccgg gcaggtgatg
     2881 acgtggacga tatcagggcg caggtcggag agcgccttct cataatcgtc atatgcggct
     2941 ggcacgccgt atttctggca aaacgccgca aggcgcccgg catcgaggtc gcacgcgccc
     3001 gcgagttcca tgttgcgcaa ctcgcgatag gcaggcgcgt gccactgggc gcgcgcaccg
     3061 caccccagga aaagcgaacg ataggttgcc atcaggcgta tcccttcaga gcgagtggag
     3121 ttcccagacc ggaaacttat ctcccttttg gcccttaggc cagcgtctcc ttcacgctgt
     3181 ctccgctgcc agcgcccgga cctgctccgc ggcctggcac gcgcccacgc cgccgttccg
     3241 gcgctcgcaa tccacgacca ccacgtactc gattgtggtc ggccgcggcg agtagcgctg
     3301 ggagctcacc cgataccgct tatagggttg gcagagaacc tggagcgggc cacgcgagcc
     3361 gaggatctcc atcacctgct ccacggagag gatgccttcg gtgctatagc tcatcagaat
     3421 gcgccggacg cgcaagcgcg agatgatggt ggcgagggca tccgccgcct gatggcggta
     3481 gttgaatgca gatcggcgct cggtacgcca gtcggtccgg atgcccgact tccccccgat
     3541 cccctcatca atccgccggc tcactggggg cttgtcccac agcaccaggg tgttcagcag
     3601 gtggtagttg ctcccatagg ggtgttggtt atagggcggg tcaagatagg caatatcggc
     3661 gcggaggtgc tctgccaggc gcccggcgtc ttcacggaaa accaggtttt cctcgccgtt
     3721 atcgagcgtg aggggagggg agagacggat gtcactgagg atgcggtaga gcgcggtgcg
     3781 cgtagcgcca ccccatcccc ggtggaagcc tttgaagagg ccgctcgtat tgctcacgta
     3841 gctcgcggag tagacgagag gtgccaggag gaaggcgcgc tcacgctcgt ccaggagacc
     3901 ctctctctcc caggcagcca cctgctcgcg gatggcatcg atccggcggc cgttgcgccg
     3961 ggtgaagaag aggcgctcac ggtccggatc gggccgctca tcatcctcgg ggcagtagtg
     4021 cgtggcgata tagccatcca ctggcggcag ggcgttcagg taatgaaaga gggcgtccgc
     4081 gccgccccac cgcctgaact gtggcgggtg attgcactcc acgtaggcga cgttgagcac
     4141 gtaggcatac ggctcccaat cgttggagag gacgcggtag ccgagctgct tcgcgaggcg
     4201 agagaccacg ccactgccgg cgaagagatc gaggaaggtg ccctcgccaa tgccagcgag
     4261 cttgagcgcc tccccgagga gggggagcag ctttcgcttg ttaccgatat aagggatgag
     4321 ctggtgatag aggtactcgt cggtgcgaca ggcagcatgc acgcggggcg tgtgatgcac
     4381 ggacgcgggc agggtgacgg cggatatggc agcactcatc atctcttcaa cgggtcgcgg
     4441 gaagtctcgc gtctcaacca taacacaggc cctcgctgct gtcaaaggcg cccggatgtt
     4501 tgctgcctct ggctgaggga ggtttccctg gcctgacaag ggggaatgag cttacgcgga
     4561 ggtacaaccc cgcaaggagg gagcagacca tgaggcacgt cgccaggcac tggcgcctgc
     4621 cggcattcgc agccgcgctc gcgcttgcct ttctggccgg ccggttcgca acgccaggcg
     4681 gcccatccca agttcatgcc gagccctctg tctcccacgg ccaggaggcg attatcgccg
     4741 tcgcgcgcgc ggtttcccct acggtggtgg gcgtcatccg catgcaagat ggcgagcgcg
     4801 aggggcttgg ctccggtgtc atcgtgggcg aggatggtct catcctcacc aacaaccacg
     4861 tcgtcgcggg cgcgcgcgag cttgtggttg cgcttgccga cggacgcgag ctgccggcta
     4921 ctgtggtgaa caccgacccc atctcggaac ttgccctggt gaaggtggat gcgaaaggcc
     4981 tgcctaccgc caccttggga gactctgatc aactgcgcgt ggcccagacg gccatcgcta
     5041 tcggcaatcc gcttggtttc gagcgcactg tgaccgtggg cgtggtcagc gccatcaacc
     5101 gccacctgcc agaccggcgg gcggagctgg acaacctgat tcagacggac gcggcgatca
     5161 accccgggaa ctccggcggg ccattggtag acatccaggg gcgcgtgatc gggatcaaca
     5221 cgctcgtcgt gcgtggcccc gtcggtgcgg gcgggcttgg cttcgcgatt cccgcttcta
     5281 cggcgcgaga cttcatcagc gatgtgcagc gttatgggcg cgtggtgcgc acctggcttg
     5341 ggatcacgcc ggtcgatatc gaccgggaga ttgcccggcg cttcgacctc ccgatcaccg
     5401 aaggcgtcct cattcggaga gtcttcagcg atagcccggc cagccgtgcc gggctacggg
     5461 agcgcgatat tgtggtcaac gcgaacggca ccgtcgtgcg caatctgggc gacctgcgcc
     5521 gcgtgatccg gagcaaacgg gtgaatgatc cgatgaccct caccatcctt cgcgatggtg
     5581 agcgcatccg cgtgatgctc cgactggctg aacgcccccc ggatgccggg aggtagcagg
     5641 gccgcgccgg cgccgggtcg acgatactgc ggatgtaagg gacggacgcg gataggtggg
     5701 tgcggggagc cagacactcc ctgccccccg gccccgcgcc gatgccgata ccgcggatga
     5761 gggggacggg cagcttgggt tgcgcggcgg cctcggggtc gagaccaggc ccgccgcgtg
     5821 gctctcggag gccccctagc ggggtcggat gcgctgttga cacttgggac gggtggctgg
     5881 tataatccgg cctggtggtc cgaacaccgt ttgggttatc tcggcgcgac ggcggcccgg
     5941 cgtcgccggg ccagggatga gcccaaagga tcggaagggg aattgagtgg tgaggagagg
     6001 agggcgggta gccccgcccc gctcccgtca ggagcctacc gggtctgcct cggcgggcag
     6061 caatccaaat tcgcgcaagc gtcgttcggt cgcgacatcg caggcggatt ggtcctcctc
     6121 actgcatcgg ccggactgac atagggtgta gcaggtcagg gtcatgatgt tcttacggcc
     6181 gaagcaatct tgccgtcctc gcttcttttt tggcattcga caacctccac tgtcctgcac
     6241 cattacggta tcttaccaca gagagggcaa ccgtgtcaaa cgatagtttg catgttccgg
     6301 cgatccggaa cacactgggc gatccagccg gcgcccgccc catcgagatt ccatggcatc
     6361 tggcagcgtg aaggggcttc tgggccctcg gggaaggctg gcggaacgaa tccgcggttt
     6421 cgagtttcgg cgcgagcagc tcgagatggc agaagccatc gcggcatcgc tggccgaggg
     6481 ctcgcacgtg cttgccgagg cgggcaccgg agtaggcaag acgttcgcct atctggtgcc
     6541 ggcgttgctc tacctgcgcc acggccgcaa agtcgttctc tccacgcaca ccatcaacct
     6601 ccaggagcag ttgttggaga aggacgttcc cctgttacgc gacgcgctgc cggatcgctc
     6661 cttccgggtc gcgttgctca aggggatggg caactacctc tgcttgcaga atatggatgg
     6721 tgtcgcccgc ctggatctgc tggagcacga ggagtggcag cgcctgcggc tatgggcg
//
LOCUS       DAOSCV010000097         6716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7300_length_6716_cov_2.995196, whole genome shotgun sequence.
ACCESSION   DAOSCV010000097 DAOSCV010000000
VERSION     DAOSCV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_6716_cov_2.995196"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PLY56_03705"
     CDS             complement(<1..903)
                     /locus_tag="PLY56_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ20615.1"
                     /translation="MEHPAAGRRQDRGLHILSGLNLAGLVTVWGLETLLGERHWLTTL
                     LAYAPQHPFLVPTLGLLVWGLTRRSWLPVAINTGAGVLFAFALMGATVPLGARGQAAG
                     ERVRIMTLNIHHASEGVERVAALVRELQPEIACFQETNPHWVWDDPIPELQRLLPEWH
                     LVRHGELATFSRLPILSHRLHPMPGKHRREILESVVQVGEEQLTVLNVHFNTAARPRA
                     FLRNPRSGLAYLHDSARVRSAQMEALLEVAERQPSHLVIAGDFNQRVPRRRQPVFVAE
                     RLGEVLAGWKIHTAGALPNGPHIDH"
     gene            1369..3114
                     /gene="recJ"
                     /locus_tag="PLY56_03710"
     CDS             1369..3114
                     /gene="recJ"
                     /locus_tag="PLY56_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOJ20616.1"
                     /translation="MDLQHRKWVVCPRIPDATLATVPEIPPLLAQLLYNRGILNGDAS
                     LNRDVLAQFLQPRWDGGLHDPFLMADMGRAVERILAAVERRECIGVFGHYDADGITAL
                     VLMTSVLKRLGARVVPYMPRRSDDYGVNDNGIAELAAQGVTLLISVDCGIRSFLAPET
                     AARHGMDVIITDHHTVLQGDAGDELPAAFAVLNPMRADCPYPCKSLAGVGVAFKLAQA
                     LLRAHAQRTGEPETHYAKWLLDLVCLGTVGDVSDITGENRTLVTLGLEILKRSKRPGV
                     QALLGVAGTNVSKLNVETVGFQLAPRINAAARMEDPWLSVSLLLTTDPAEAMSTARQL
                     NTLNNKRQEITNQAMAEIEKRYGETLLEHNAIVVDGSWPAGIIGLIAGKLREAYSRPA
                     AVIEISNEEEAHGSARSCPGFHISDALRDCADLLSRYGGHAQAAGFALPRQNVDAFRE
                     RLQTLADERLRPEELVPVLAIDALLDPSEITLDLLRVIQQLEPCGPGNPSPLFGMRGV
                     RVASAETFGTTGDHLRLMLRAPDRTLRATGWRMGLLAEQFPPGAAVDIAFALKEDSYV
                     GLALDIQDVAAAGER"
     gene            3111..4184
                     /locus_tag="PLY56_03715"
     CDS             3111..4184
                     /locus_tag="PLY56_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HOJ20617.1"
                     /translation="MMLRARWGVIAAILSARAGSIEARVEFSDAPEESGPALAYPELT
                     GPIAVGDRVLVNTTAVHLRLGTGGLHFIMAVDRGEPSLPPPGPGHIMKLRYTPLQQAV
                     LTAEERATAGELTLPEELGGTPVVAAELHSQVAAVAAGIRATAPAARVAYVMPDAAAL
                     PLALSHLIPRLRKAGLIHTTLTCGQSFGGDLEAVSVPSALVAAKAAGADVILVAQGPG
                     NAGTGTPLGFSGIAQADWLNAAHSLGGVPVAALRLSFADARPRHQGVSHHSLTVLGRF
                     TLCPAVVAVPLLPDPLARQIGSQLAMLPARHTIEMHDGEPALALLASLGVAVTTMGRS
                     VEAERAFFLAAGAAGVAAARRLG"
     gene            4375..6204
                     /gene="glmS"
                     /locus_tag="PLY56_03720"
     CDS             4375..6204
                     /gene="glmS"
                     /locus_tag="PLY56_03720"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOJ20618.1"
                     /translation="MCGITGYVGSRHALPLALEQLRKLEYRGYDSAGVAFPDATRVQV
                     VKSVGKIAGLDPLVAGLGQGCGTAVAHTRWATHGRPSTENAHPHADCRGQIAVVHNGI
                     IENYLSLRARLEAAGHLFQSETDTEVLSHLIEEYYAGKMEEAVAAALSEVRGSFAIAV
                     VSTYEPDSLVVARRDSPLVIGLGRGEYFLASDIPALLAHTRDVIILEDDDLGVLSPAG
                     MRLRKLDGTMVQRTPMHITWDVGAAEKGGYEHFMLKEIREQPRTIRDTMRGRVMPDHQ
                     VDLSAELNIPAERWRSFDKISLVACGTAYYAGYAMRYFFERVLRIPVELEVASEFRYR
                     DPTIDERTLFVVISQSGETADSLAALRLAKSRGATALGVVNVVGSSIAREADSVLYTW
                     AGPEICVASTKAYTSQLVALYLLGLHAARLRGSFGAEETAALVAQMKQLPDLVSRVLD
                     CDESVRQMAPSFAGAADFFVLGRGLDYAVALEGALKMKEISYIHGEALAAGEMKHGPL
                     ALVSQGTPVVCLVTQEALREKMLSNVKEVKARGATAIGIVREGDTESAMSMDHVIHVP
                     STDDWLMPMVAVVPLQLLAYYVARELGREIDQPRNLAKSVTVE"
     gene            6286..>6716
                     /locus_tag="PLY56_03725"
     CDS             6286..>6716
                     /locus_tag="PLY56_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20619.1"
                     /translation="MFLRFLVCLVVAWMASAPACVAGPVAIREEGERLFLGNGLAEYE
                     FRKDQGYDLVAVRLEKGGEPLLLTSAHLVYCEDGVWMKECGADPGADAGAGFVGHQVE
                     RVSEKGRSGLRVRAQNDRLRVEKSFLLDADGPGLQVRYHIET"
BASE COUNT         1059 a         2165 c         2315 g         1177 t
ORIGIN      
        1 gtggtcgatg tgcggtccgt tcggcagggc cccggcggtg tgaatcttcc acccagccag
       61 gacctcgccg agccgctcgg cgacgaagac cggttggcgg cggcgcggga cgcgttggtt
      121 gaaatctccg gcgatcacca ggtgactcgg ctggcgctct gccacctcca ggagtgcctc
      181 catctgcgcg gagcgcaccc tggcggagtc gtggaggtag gcgaggccgg agcgcgggtt
      241 gcgaaggaac gcccgcgggc gcgcggcggt gttgaagtgc acattgagca cggtgagctg
      301 ctcctcgccc acctgaacca cgctctccag gatctcgcgc cgatgcttcc cgggcattgg
      361 atgcaggcgg tgagagagga tcggcaggcg agaaaaggtg gccagctcgc catgccggac
      421 gaggtgccac tccggcagga gccgctgcag ctccgggatc gggtcgtccc acacccaatg
      481 cggattagtc tcctggaaac aggcgatttc cggctgcagc tcgcggacca gggccgcgac
      541 acgctcaacc ccttcgctgg cgtggtgaat gttgagcgtc atgatccgca cgcgctcgcc
      601 cgctgcctga ccccgcgcgc ccagtggaac cgtcgcgccc atgagcgcga acgcgaaaag
      661 gacgcctgcg ccggtgttga tcgccacggg aagccacgaa cggcgtgtca gcccccagac
      721 aagcaagcct agagtgggga cgaggaaggg gtgttgcggc gcgtaggcca ggagcgtggt
      781 gagccagtgt cgctcgccca gcagcgtctc cagcccccat accgtgacaa gccccgccag
      841 gttcaagcct gataggatat gcagcccccg atcctgtcgt ctccccgcag ccggatgctc
      901 cattggctct ccgcgtttcc tcctatacgt tacgctacgg cagcgctgca ccccaggcac
      961 gttctttgcc ggatgcagct tgcgtccggt acccagttcc atctgctggc ccggatgcct
     1021 ttccaggagg agcgccgcgg gtagcggacc cccggcgcgt tgtcggtact tacgaaccca
     1081 cggagaggct gttgataggc aggattctga gccctgggaa gccaacggag aaagcgggca
     1141 agccacggtg agcagcgctc gcggctcacg cgcccccgca acttccccgc tatccgatcc
     1201 tgaacgatgg catcggtgag tcgccatgtt gcctttcacg ggcaggatgg cgggttgaag
     1261 gggccccacc cggctgaccg ggacgagggc atgcccgcga gagtggctgc cggcgcctct
     1321 tgtgaccgag aagagtggcc catcggccgt tttggaggga gacagttctt ggatttgcaa
     1381 caccgcaagt gggtggtctg cccgcgcatt ccggatgcga cgctcgccac cgtgcccgag
     1441 atacccccgc tcctggccca gctcctttac aaccgcggga ttctgaatgg ggatgcgtcg
     1501 ctcaaccggg atgttctcgc gcagtttcta cagccgcgct gggacggagg cctgcacgat
     1561 cccttcctga tggcggatat gggcagagcg gtggagcgca tcctggcggc ggtggagcgg
     1621 cgcgagtgca tcggtgtttt cgggcactac gatgccgatg gtatcaccgc gctcgtcttg
     1681 atgacgagcg tgctcaagcg tcttggtgcc cgggtggttc cgtatatgcc tcggcgctcg
     1741 gatgactatg gcgtgaacga caacggcatc gcggagctgg cggcccaggg cgttaccctc
     1801 ttgatcagcg tcgattgcgg gattcgctct tttctcgctc cagagaccgc cgcacgccat
     1861 ggaatggacg tgatcatcac cgatcaccac actgttctcc agggagatgc tggcgacgag
     1921 ctgccggccg cgttcgcggt gctcaacccg atgcgcgccg actgccccta tccgtgcaag
     1981 tcgctggctg gggtgggcgt ggcgttcaag ctggcacagg cgttgttgcg cgcgcatgcc
     2041 cagcgcacgg gcgagccgga gacgcactat gccaagtggc tgctcgatct cgtctgtctt
     2101 ggcaccgtag gcgacgtctc cgatatcacg ggcgagaacc ggaccctggt cacgctcggc
     2161 ctggagatcc tgaagcggag caagcgtccg ggagtgcagg cgttgctggg cgtggccggc
     2221 accaatgtga gcaagctgaa cgtggagacg gtcggcttcc agcttgctcc acgcatcaac
     2281 gcggccgcgc gcatggaaga cccgtggctg agcgtctcgc tactgctcac cacggatccc
     2341 gccgaggcga tgtcgaccgc gcgccagctg aacacactca acaacaagcg ccaggagatc
     2401 acgaatcagg cgatggccga gatcgagaag cgctatgggg agacgcttct cgagcacaac
     2461 gctatcgtcg tggatgggag ctggccggcg gggatcattg gattgatcgc gggcaagctg
     2521 cgcgaggcgt attcccgccc ggccgcggtc atcgagatca gtaacgagga agaggctcac
     2581 ggatcggcgc gaagctgccc cggcttccac atctccgacg ccttgcgcga ttgcgccgat
     2641 ctcctcagcc gctatggcgg gcacgcgcag gccgcgggct tcgctctccc tcgccagaac
     2701 gtggatgcct tccgcgaacg cctgcagacc ctggcggacg agagactgag gcccgaggag
     2761 ctggtgcctg tgcttgctat cgacgcgctt ctcgacccga gcgagattac gctcgacctc
     2821 ctccgtgtga tccaacagct tgagccatgc gggcccggga acccatcgcc tctctttggg
     2881 atgcgagggg tgcgcgtggc ctctgcggag acctttggca cgacaggcga tcacctccgc
     2941 ctgatgctac gggcgcccga ccgaaccctg cgcgccaccg gatggcgcat gggtcttctg
     3001 gcggagcagt tcccgccagg cgcggccgtg gatatcgctt tcgcactgaa ggaggactct
     3061 tacgtcggat tggccctcga tattcaggac gtggcggctg ccggagagcg atgatgttgc
     3121 gtgcgcgctg gggcgtcatt gccgcgatcc tctcggcccg tgccggttcc atcgaggcgc
     3181 gtgtggagtt ctcggatgcc cccgaggagt ctgggccggc gctggcctac ccggagttaa
     3241 ccggaccgat tgctgttggc gaccgcgtcc tggtgaacac gactgcggtt cacctgcgcc
     3301 tgggcaccgg cggtctgcac ttcatcatgg cggtggatcg tggcgagccc tcgctgcctc
     3361 cccctgggcc cggccacatc atgaagctgc ggtacacccc tctccagcag gcggttctga
     3421 cggccgagga gcgcgccacc gccggcgagc ttacgctccc ggaggagctg gggggtacgc
     3481 ccgtagtggc cgctgagttg cacagtcagg tggccgctgt ggctgccggc atccgtgcca
     3541 cggctcccgc agcccgggtc gcctatgtca tgccggatgc ggccgcgctc ccactggcgc
     3601 tcagccattt gatccctcgc ctccgcaagg cgggcctgat tcacaccacg ctcacctgcg
     3661 gccagtcgtt cggtggcgat ctggaggcgg tctccgtgcc ttccgcgctg gtggcggcca
     3721 aggcggcagg cgcggatgtg atcctcgtcg cccaggggcc gggaaacgcc ggcacgggca
     3781 cgccgctcgg cttttccggc atcgctcagg ccgactggct caacgcagcg cactccctcg
     3841 gcggcgtgcc ggtcgccgcg ctccgcctca gctttgccga tgcccgcccc cgccatcagg
     3901 gcgtcagcca ccactctctc accgtactgg ggcgttttac cctctgcccc gcggtggttg
     3961 cggtccccct cctgccagat cctctcgcgc ggcaaattgg cagccaactt gccatgcttc
     4021 ccgcgcgaca caccatcgag atgcatgatg gcgagccggc gctggctctg ctggcgtccc
     4081 tgggggtcgc ggtgaccacg atgggtcgct cggtggaggc cgagcgcgcc ttcttcctgg
     4141 ctgccggcgc ggccggggtc gccgccgcca ggcgtctggg ctagtggatg cggttcgcag
     4201 ggggcaggtg gcaggtgaca gggggcggtt caccattcgc tgacgaacgc aggcaggcgt
     4261 cccggcgcgc tgcacccgcc ggcgccttgc acgctgcgcg cagggtcccc cctcactcct
     4321 tcactctgtc atcccctcat ctgccgatgg atcggggaag gggaggactg tcgagtgtgt
     4381 gggatcacgg ggtatgtcgg ttcgcggcac gcgctgccac tggcgctgga gcaactgcgg
     4441 aaactggagt accgaggcta tgactccgcc ggggtggcct ttccggatgc gacgcgcgtc
     4501 caggtagtca agagcgttgg gaagatcgcg gggctcgacc cgctggtcgc cggcctgggc
     4561 cagggatgcg gcacggcggt ggcgcacacg cgctgggcca cgcatggccg gccctcgacg
     4621 gagaatgcac acccgcacgc agactgccgc gggcagatcg cggtggtgca taacggaatc
     4681 atcgagaact atctctcgct gcgcgcccgt ctcgaggccg ctgggcatct cttccagtcg
     4741 gagacggata ccgaggtgct ctcgcatctg atcgaggagt actacgcggg caagatggag
     4801 gaggcggttg cggcggcgct ttccgaggtg cgcggctcct ttgccatcgc cgtggtgagc
     4861 acctatgaac ccgatagcct tgtggtcgcc cggcgcgata gccccctggt gatcggcctg
     4921 ggaagggggg agtacttcct ggcgtcggac atcccggcgc tgttggccca cacgcgcgac
     4981 gtgatcatcc tggaggatga tgacctaggc gtgctctcgc cggctgggat gcgcctgcgg
     5041 aaactggatg gcaccatggt gcagcgcact ccgatgcaca tcacctggga tgtcggggcc
     5101 gcggagaagg gcggctacga gcacttcatg ctgaaggaga tccgcgagca gccgcgcacg
     5161 atccgagata cgatgcgtgg ccgggtgatg cccgaccacc aggtggatct cagcgcggag
     5221 ttgaacatcc ctgccgagcg gtggcgctcc tttgacaaga tctcgctggt cgcctgcggc
     5281 acggcctact atgcgggcta tgccatgcgc tacttcttcg agcgcgtgct gcgcatcccc
     5341 gtcgagctgg aagtggcctc cgagttccgg tatcgcgacc cgaccatcga cgagcgcacc
     5401 ctcttcgtgg tgatcagcca gtcgggcgag acggccgaca gcctggccgc cctccgcctg
     5461 gcgaagagca ggggcgcgac ggcgctgggc gttgtcaacg tcgtcggatc gtccatcgcg
     5521 cgcgaggcgg attcggtgct ctacacgtgg gccggccctg agatctgcgt cgcctcaaca
     5581 aaggcctaca cctcccagct ggtggccctc tatctcctcg gattgcacgc ggcgcgcctg
     5641 cgtggtagct tcggggccga ggagacggcc gccctggtgg ctcagatgaa acagctgccc
     5701 gacctggtaa gccgggtgct ggactgtgat gagtccgtgc gccagatggc gcccagcttt
     5761 gccggagccg cagatttctt cgtgctgggg cgcggcctcg attatgccgt cgcgctcgag
     5821 ggcgcgctga agatgaagga gatctcctac atccacggcg aggccctggc cgcgggtgag
     5881 atgaagcacg gccccctggc cctcgtctcc cagggaaccc ccgtcgtttg cctcgtaact
     5941 caggaggcgc tgcgcgagaa gatgctctcg aacgtcaagg aagtgaaagc ccggggggcg
     6001 acggcaatcg gtatcgtccg cgaaggtgac accgagtcgg cgatgagcat ggaccacgtg
     6061 atccatgtgc catccaccga cgactggcta atgccgatgg ttgccgtggt gccgctgcaa
     6121 ctcctcgcct actacgtggc gcgcgagcta gggagggaga tcgaccagcc tcggaatctg
     6181 gccaagagtg tcaccgtgga gtagcgccaa agcgaccagg atggcaagcg cctgaaaggg
     6241 gaacccgcct ctcccacgtc gaatcccgtg ggggaggtgg gtcttatgtt tctccggttt
     6301 ctcgtctgtc ttgtggtagc ctggatggca tcggccccgg cgtgcgtcgc ggggccagtt
     6361 gccatccgtg aggagggcga gcggcttttc ctcgggaatg ggctggccga gtacgagttt
     6421 cgcaaggacc agggctacga tctggtggca gtccgcctgg aaaagggagg cgagccgctg
     6481 ctcctgacca gcgcccacct ggtctattgc gaggacgggg tttggatgaa ggagtgcggc
     6541 gcggatccgg gcgcggacgc cggcgctggc ttcgtcggcc atcaggtgga gcgcgtctcg
     6601 gaaaaggggc ggagtggact gcgtgtccgc gcccagaacg accggctccg ggtcgagaag
     6661 agcttcctcc tggatgcgga tggcccgggt ttgcaggtgc gctatcatat cgagac
//
LOCUS       DAOSCV010000098         6618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7437_length_6618_cov_3.680177, whole genome shotgun sequence.
ACCESSION   DAOSCV010000098 DAOSCV010000000
VERSION     DAOSCV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7437_length_6618_cov_3.680177"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLY56_03730"
     CDS             complement(<1..561)
                     /locus_tag="PLY56_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ20620.1"
                     /translation="MELEGKSALVTGGGRGIGRATALALARQGCAVAVCARTREEVEE
                     TARQVRAAGGKALAFTADVSLEADAERVVAETISQFGGLDILINNAGVFVLASVLETP
                     VEEWDRLMAVNARGAFLFSRAALRHMRERRAGAIINISSTAGKRAYPEQSAYVASKHA
                     LLGFTKALALEAQPYGIRVHAVCPGVV"
     gene            complement(737..1570)
                     /gene="srlD"
                     /locus_tag="PLY56_03735"
     CDS             complement(737..1570)
                     /gene="srlD"
                     /locus_tag="PLY56_03735"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HOJ20621.1"
                     /translation="MLGPAPTPRGPKSRPFRRLEGRTAMVTGAAQGLGEALASRLAAE
                     GCHVAIADINEAKAEETAARIRKEYGVQALAFGMDVTDDAQVGQVVDQVVAAFGGLDI
                     MICNAGILIAGDAVEFDAEKWRKVIDVNLIGYFITARHAARVMQEQRRGSIIQINSKS
                     GKKGSFKNSAYAASKFGGIGVTQSLALELAPFNVRVNSVCPGNLLDSPLWVNSLYEQY
                     AKRWNLTVEEVRRKYEEQVPLGRGCAYEDVANVVVFLASDESSYMTGQAINVTGGQEM
                     R"
     gene            complement(2978..3199)
                     /locus_tag="PLY56_03740"
                     /pseudo
     CDS             complement(2978..3199)
                     /locus_tag="PLY56_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            complement(3656..4150)
                     /locus_tag="PLY56_03745"
     CDS             complement(3656..4150)
                     /locus_tag="PLY56_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20622.1"
                     /translation="MELRRLTLAELSQYLEGRTPGLQDLEPADRDSVVRMACRLAEQG
                     IASRFILSAVRDTVDLMRAPEVPPDLERPASVELPPADDRQQAARRLKSYTQATLREA
                     RAARRSNEIRHMRGVLRGIDRGHLRETLGDEGESLCREIDTVLSRLSLPVRRGRPRSQ
                     RIAA"
     gene            complement(4582..6534)
                     /locus_tag="PLY56_03750"
     CDS             complement(4582..6534)
                     /locus_tag="PLY56_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ20623.1"
                     /translation="MFRARLPFCQVFCLGLMVMLWSPRVWAGQALPDKIALSESFEGE
                     LPDFHTYQATYAADEMRARTGKRSLRVMPTGGSGGAYFKLQGLIDPTRDYEFSVWVHA
                     ESAHAASLYISASDGKTRHTKGRARREAKQGDWVRLVGHVRAKEWRETDQEIMLAMTG
                     SQEIWVDDVTLLATTVPDPPSQVYPEVERRLRAEADKRPIRLARGKRIVARASNGAFA
                     SDSAVMEARAVQSDTLQITPNGLLTFAIDADEPLYVTGSLLLTPDSDLRPGLRVTVLC
                     NTTVVAAPMVEAEAWEGVGNPLTGPAPEITGTRPPDRVPLVEWRLPKGRSYLTLAGPH
                     FRPGGTFRELELRALERKADAPSYQFALFSDTHLSTASPGEWMNVKMGNATAPELATT
                     LQSLAKEGIDFAVLAGDMTDRGTREDVQVLAGVLEKAPLPVAGCIGNHDAYHSSSRPD
                     LLELCGRLFPGGRTDYVIQKPPLRFIVLDASYWRTPEGDFVDRYDAKNARGIGLRPAQ
                     VEWLRKTLTEDTRTPTVVIWHYAFTNRRGNSSCGYKLPGAICLSSQDVLGILEAAPNV
                     VATLCGHAHWNEVNRVGRVTHLQNPSFAEWPNAFRVVRVYPDRLEWEVRQPGNRGFIR
                     ESFLAHKALSWMLSTGEGDLAGEVRF"
BASE COUNT         1087 a         2340 c         2087 g         1104 t
ORIGIN      
        1 gaccacgccg gggcagacgg cgtgcacccg gatcccgtag ggctgggctt cgagcgccag
       61 cgccttcgtg aagccgagaa gggcgtgctt cgaggcgaca taggcgcttt gctccgggta
      121 ggcgcgcttc cccgcggtag aagagatgtt gatgatcgcg ccggcgcgcc gctcgcgcat
      181 gtgccgcagc gccgcacggg agaagaggaa agcgccgcgc gcgtttaccg ccatcaggcg
      241 gtcccactcc tccaccgggg tttcgagcac ggaggcgagc acgaagacgc ccgcgttgtt
      301 gatcaggatg tcgaggccgc cgaactgcga aatcgtctcg gcgaccacac gctccgcatc
      361 ggcttccagc gagacgtccg cggtgaacgc cagcgccttt cccccggccg cgcgaacctg
      421 ccgggcagtc tcttccactt cttcccgggt gcgcgcgcag acggcgacgg cacacccctg
      481 gcgggcgagc gccagcgcgg tcgcccgtcc gattccccgc cctccaccgg taacgagggc
      541 gctcttcccc tctaactcca tcttctcacc atctccggcg gcaccatccg ctctgcgtcg
      601 tcgcggccgc caggaagccg ccggttaacc aacacaccgg gtgccggaat ggcgcttcgc
      661 gccatccggc acccatactc cgccaggcga aacgcctgcg acgcctccgg catgcgggat
      721 tcccacaccc cgagggctac ctcatctcct gcccgccggt gacgttgatc gcttgaccag
      781 tcatgtacga tgactcgtcc gaggccagga agacgaccac gttcgccacg tcctcatacg
      841 cgcacccgcg cccgaggggc acctgctcct cgtacttgcg gcgcacttcc tcgacggtga
      901 ggttccagcg cttcgcatac tgctcgtaga ggctgttcac ccacagcggc gagtcgagga
      961 ggttcccggg gcacacggag ttgacgcgca cgttgaacgg cgccagctca agcgccaggc
     1021 tctgggtgac gccaatgccg ccgaacttgc tggccgcata ggccgagttc ttgaagctcc
     1081 ccttcttgcc cgacttcgag ttgatctgga tgatgctccc ccggcgctgc tcttgcatga
     1141 cgcgcgcggc atgccgcgcc gtgatgaagt aaccgatgag gttgacatcg atcactttgc
     1201 gccatttctc agcgtcaaac tccacagcat cgcccgcgat cagaatgccc gcgttgcaga
     1261 tcatgatatc gagcccgccg aaggcggcca cgacctggtc gaccacctgg ccaacctggg
     1321 catcgtccgt cacatccatg ccgaaggcga gggcctggac gccgtactcc ttgcggatgc
     1381 gcgccgcagt ctcttccgct ttcgcctcgt tgatatcggc aatggcgaca tggcatccct
     1441 cagcggcaag gcgcgaggcc agtgcctcgc ccaggccctg cgcggcgccc gtcaccatcg
     1501 ccgtccggcc ttctaatcga cgaaaggggc gcgacttcgg ccctctcggc gtaggcgctg
     1561 gaccgagcat cttctcgaag agcgccgctt ccgcctcgcg cgtccactcg cccgcggggc
     1621 tcagcttcgc ggccacttcg gggaggcgat ccttcagttc cggaagcggc gtgagcggca
     1681 ggttccggat ctgcgggaag atgacgatct tgcccgggaa gcgcgctgcc ttgaccgcgg
     1741 ccaagccgtc atgcgctgcc tcgatgccgc caatggcagc taccgagcgg ttggggctga
     1801 gcgtgccttc ctcggtcgcg ccgagcgtgc tcttcagatc ctcgatccgc gagccgctgg
     1861 tgccgataaa gcgcacgtcc ttgcgcaccg cgtcgagcac tggcacgcgc gccatcgtcc
     1921 ccttgggaac gccggcgaag atgttgagga ggcccctctc gctcaggtgc gtggcgcagt
     1981 ggctgatcac tgccggaacc ggcaccatca ccgcgacatc ggtgaatccc tggccgcccg
     2041 tcacttcgcg cagtgcctgg tcgaacgcct cgggcgtcat ctccttgggg ttgagggcaa
     2101 ccatctttgc gccgcgcgcc tcggccagcg ggccgaaacg ctccagcacg acatttagac
     2161 gctcgccatc aatatcggtc acgacgacca ggctcgggcc gtccggcttc tcggcggcgc
     2221 gctgggcatg catctggccc atcgggccgc ccgcgccaca gatccaggcg cggccgcccg
     2281 gcatgaggtc cgaggaacgc gagcgcgtgt aggcctcggc aatgtcatca ctgcgggtgc
     2341 ccaaatgcac caggaaatca taatggatgc gaccgacatc cacttcgacg taggcgtcgc
     2401 cgtcagcagc ccccaccagg ttgaggatgc cgtgcttgcc cagcatggta tccagctgcg
     2461 cgaccatcgg tgcatccgcg ccaagggcca caatgtcatc gaaccccgcg ccaccggtct
     2521 gatccgcctt cagtgcctcc aggtccagcg aggcaagcgc cccggtgcgc acgaccgtcg
     2581 cacccgccgc cttggcctgc gcttcgaggc ggtcggcaat cgccgccggg acatcggtga
     2641 ggatgacgac gcgcggatgc gagtctgcat ccaggccacg gctgacatgc agcgaggcgg
     2701 gcacgctggc tgcggcgggt ccaccgacca cccagagcgc accgccgttg aggagggtcg
     2761 accggtgcga cgcgcggtag ctagcgacca cgcacgccca cggctcggag agcgccgcct
     2821 cggcatagcc tgtttccggc ttcacgggca gcagatagca gccctcatcg ccatcgatca
     2881 tctccttggg gatgacgcca aactgctcga gggcgcccgg aagctggtag ccgtaggcca
     2941 cattcttgcc cttgtagtag acatccgcct gaacgatgaa gcggtcgccc ggcttgaact
     3001 gcccgcggcg gttctcgccg acggccacca ccgtcaccga ggcttcgtgc ccgaggatga
     3061 ccggttcgcg ctccatgtcg cgccccagga tgcgcgggtg ttggttcccc tgcgtcagaa
     3121 ccttgatatc gctgaagcag aggccggcgg catccacgcg caccagcagc tcatcgggcc
     3181 ccggctgcgg cacgcgcatc tcgaccggct tcccctcgtc gcccaggttc tccaggcccg
     3241 ctccatacag cggccatgcc aacatcgtag acggcgtcgc cgttgggttc tctcgatact
     3301 ccttcagcct atcgctcacg gcaatctccc ctgaatctta caaaatcgcg cgccgcccac
     3361 caccgagcag gtgtgccagg cacgccaaca caatcccacc ggagtagagt tcggctcccg
     3421 atgccgaggc acctgctttg agttcgtgcg aagcgcttga agaggacacc ttcgctgccc
     3481 gcgcgagagg gcagcgttgc tcccgatctc cgctgcgggg aatccgggca ttggcccgag
     3541 caagccaggc gtctgaggcg gatctccccg cggccagggc gtcgctagac ccggtcaggt
     3601 ggctcgatca gcccgagcca cctgaccgag gtttctgaaa agcgttcaga acggcttacg
     3661 ccgcgatccg ttgggagcgc ggccggccgc gacgcacagg gagggacaga cgcgatagaa
     3721 ccgtgtcgat ctcgcggcag aggctctcac cctcgtcacc gagcgtctcg cgcaggtgac
     3781 cgcggtcgat gccccggagc acgccgcgca tgtggcggat ctcgttgctg cgccgggcgg
     3841 cgcgggcctc gcggagggtg gcctgggtat agctcttcaa ccggcgcgca gcctgttgcc
     3901 ggtcgtcggc cggagggagt tccacagagg cgggccgctc cagatcgggc ggcacctcgg
     3961 gggcgcgcat caggtcgacg gtgtcgcgga cagccgaaag gataaagcgg gatgcgatgc
     4021 cttgctcggc gagccggcac gccatgcgca ctaccgagtc gcgatccgcc ggttcgaggt
     4081 cttgcaggcc cggggttcgg ccctccagat actgcgacag ctccgccaaa gtcaagcgtc
     4141 tcagttccat cctcgtctcc ttcccgtcac gagatgccgc acccggcacg gattgcagga
     4201 atcctcccac cggatgcctc atcctacgcc tgctcgcgct ctgtcagttc actgagaatg
     4261 agccggcagg gcgctcagaa ccttcctgga aggctctctc ccaaccgaca aaaaagggag
     4321 cccggtctaa cccagagctc cctcactgga tcgggacagc atgtgaggga aagaagtcct
     4381 cctgcggcct ccgcaagcgc tctcgcctca cagcctccca tccatccgcg ttcatcgcct
     4441 tggggcggcc cggccgctct gccttgcagc ctcgcgggcc ggcgcccact ccatgaaatc
     4501 tactataccc taagacccct ttggtgtcaa ggaagtttca cgcgctaagc acgtggctgc
     4561 catccgcaca cagcccgtgg ttcagaagcg cacttctccg gccaggtcgc cctcgccggt
     4621 cgagagcatc cacgagagcg ccttatgcgc caggaacgac tcgcgaatga agccgcgatt
     4681 gcccggctgc cgcacctccc actccaatcg atccgggtaa acgcggacca cgcggaacgc
     4741 gttcggccat tcggcaaaag aggggttctg gagatgggtg acccgcccga cgcggttgac
     4801 ctcgttccag tgcgcgtgcc cgcagagcgt ggcgaccacg ttgggagcag cttcgaggat
     4861 tccaagcaca tcctggctgc tcaggcagat cgcgccgggc aacttgtagc cgcacgacga
     4921 gttcccgcgc cggttggtga acgcgtaatg ccagatcacc acggtgggcg tgcgcgtatc
     4981 ctcggtcagc gtcttccgca gccactcgac ctgagcgggg cgcaggccga tgccgcgcgc
     5041 gtttttcgca tcgtagcgat ccacgaagtc gccttccggc gttcgccagt acgaggcatc
     5101 cagaacgatg aagcgcaggg gcggcttctg gatcacgtag tcggttctgc cgcccgggaa
     5161 gagccgcccg cagagctcca gcagatccgg gcgcgaagag gagtggtagg cgtcgtggtt
     5221 gccgatgcag ccggccaccg gcaagggtgc tttttcgagg acgccagcga gtacctgaac
     5281 atcctcgcgc gtgccccggt ccgtcatgtc gccggccagg accgcgaagt cgatcccctc
     5341 cttcgcgagc gattggaggg tggtggcgag ctcaggggcc gtcgcattcc ccatcttgac
     5401 gttcatccac tcgccggggc tcgccgtcga caggtgcgtg tccgagaaca gcgcgaactg
     5461 gtaggagggc gcgtccgctt tccgctctag cgcgcgcagc tccagctcgc ggaaggtgcc
     5521 cccgggccgg aagtgcggtc cggccagagt gaggtagctt ctccccttgg gaagccgcca
     5581 ctccaccagg ggcacgcggt cgggaggccg cgtgccggtg atctccggcg ccggcccggt
     5641 gagcggattc ccgactccct cccatgcctc ggcctcgacc atcggcgcgg caaccaccgt
     5701 ggtgttgcag agcaccgtga ctcgcaagcc ggggcggaga tcgctatctg gtgtgagaag
     5761 gagcgacccg gtgacgtaga gcggctcgtc cgcgtcaatc gcgaaggtga gaaggccatt
     5821 cggcgtgatc tgcagcgtgt cggattgaac cgcgcgcgcc tccatcaccg cggaatcgga
     5881 cgcgaacgcc ccgttcgatg cacgcgccac aattcgcttg ccacgcgcca gccggatggg
     5941 acgcttgtcg gcctccgccc gcagccgccg ctcgacttcc gggtagacct gtgacggcgg
     6001 gtccggcacc gtggtcgcca ggagggtcac gtcatccacc cagatctcct ggctcccggt
     6061 catcgcgagc atgatctcct ggtcggtctc gcgccactcc ttcgcgcgca catggccgac
     6121 cagcctcacc cagtcgccct gcttcgcttc tcgccgggcc cggcccttgg tgtgccgggt
     6181 tttgccgtcg ctcgccgaga tgtagaggct cgcggcgtgc gcgctctccg cgtggaccca
     6241 caccgagaac tcatagtcgc gggtgggatc aatcaggccc tggagcttga agtaggcgcc
     6301 gccagatccg cccgtaggca tcacccgcaa ggagcgcttg ccggtgcgcg cgcgcatctc
     6361 atccgcggca taagtggcct ggtacgtgtg gaagtctggg agctcaccct cgaagctctc
     6421 tgaaagcgcg attttgtccg gcagcgcctg tccggcccat acccgcgggg accaaagcat
     6481 caccatcaga cccaggcaga acacctggca aaatggtaac ctggcgcgaa acatccgtcc
     6541 cctcctctct cacggctccc atgcccggat actccccggc acccgggatt ccgcgttcgc
     6601 ccggtcgggg ctgtcttc
//
LOCUS       DAOSCV010000099         6592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7471_length_6592_cov_3.430167, whole genome shotgun sequence.
ACCESSION   DAOSCV010000099 DAOSCV010000000
VERSION     DAOSCV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7471_length_6592_cov_3.430167"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>504
                     /locus_tag="PLY56_03755"
     CDS             140..>504
                     /locus_tag="PLY56_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20624.1"
                     /translation="MGSQAIVTRIALIVMLVAGGAWIAPGTGAGAAPDPDLLGHWAFD
                     EGAGPDAMESSGRGPEGEIHGAEWVKGRFGTALRFGGSGAYVTIPELRGLDGSDEMTL
                     EAWVYWEATGRYPNIVSGGR"
     assembly_gap    505..514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <515..4651
                     /locus_tag="PLY56_03760"
     CDS             <515..4651
                     /locus_tag="PLY56_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20625.1"
                     /translation="GFLLFVSDDHCSFRMGKPGKEPFELGRDWAETGANIGRIVLGRW
                     RHLAATFKRPKLTTYLDGEPVGSATWDYPVGFSGELRIGCWSSPNVCHTGLIDEVKIY
                     RRALTPEEIRASYLKEAPRRQVAEGEAPYERIPVEATRLEPAVTLETRCARLWLDRKA
                     RVISLADKAASKEYLADAVPIARLRTAESELRPTACSYRDGLLTLEFGQGEATATVKV
                     TPRDHYLVFEVASTSGAAAEELIFLDLRVKPMPYTGNGSGLTADDEFAICLRALNLLA
                     QPVFGGTPRRMSATCSRERGIIGARAALAACPTPQIRKTLQEMTRREGLPHSPLGGAF
                     ALDAPENRYSYLFTRNISEENVDLWILMARRAGIPYIHFSGWQKTLGSYEPRPTLFPN
                     GFAGLKATIDKIHAAGLKASTHTLTGCISPTDPFASPVPDPHLAVRSTLTLAEDVDEK
                     ATTIPLAESPAGMDTIWAYASKGNAVRLGNEVVQFTGVREEPPYALTGCQRGAFGTKP
                     AAHKKGAPVSYLYSWYGTFYPDPDSPLAERVADSIARAINECGFDMIYMDGAEGMPGA
                     RGYGAAKMRNTIFSKIRRPVRVEASNGPAHHSWTMHSCMGAWDLPHWGIKPFIDLHCK
                     ANESARTGELMPAQLGWWGILGPTEDYDALLPDEIEYLCAKALGWDQALSFQGVIPSQ
                     RPVNARQDEYLTLIGRYERLRLADYFSEDVRAKLREPGAEYHLVQSPEGEWYFLPRDY
                     ASRKVTRGEEWRVTNRYARQTPKLRVQALYAAGPYDGQSVTLADFTRPGEFGEPAAAE
                     GVTISLEPSTEQVKAGTASGKLVATNRGKEARGAWARVTRTFSPPLDLRPYGGLGVWI
                     YGDGKGEVLNFQLTNIRQFWEGGMTCSEHYVTVDFTGWRYFELLFRERDADRHRDYAW
                     PYNPLYGIYRATLRRQYMPALNLYVNNVPPGETITCFISPIKALPVSKVKIANPAVTL
                     GGQRVLFPVTLESGQSIEMESPAECRLLDDRGALVERFAPRGAVPALSPGENRVAFEC
                     DAVEGYRPRARVTVITEGPPLRGRAPREKVDERWLREEYDDPRLIRAIDGKENTWEIW
                     CRQGTRGASLEAEIVVERLSPSQAGQSTEGARLIEGFEDLARFADSPENQFAKYAWDA
                     EHQGVPAKPGVAQSLELATDVVKAGKASARFTATSTRSDHAGWCARGARFSPPLDLSG
                     YAAVGWWIHGDAGGQALKLQWRDTAGRWLDLVTPVDFQGWRYVEFPLEARDGFDLSRV
                     EYLILYFNGIPAGKRVTCHVDELRVLPRPAGLREPQLTVDGRTVRFPVALSAGERLVL
                     DASGRARHYASDGRLVEVVKPSGSLPLLRPGRHQIAFGWGDKPAGDFQVKVMAVKIYR
                     "
     gene            5263..6225
                     /locus_tag="PLY56_03765"
     CDS             5263..6225
                     /locus_tag="PLY56_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20626.1"
                     /translation="MLKEITVQELDTDQFIAEKVREIQEAVGDGTAINALSGGVDSSV
                     VTMLGHRALGNRLRTVFVENGIMREGEAERIERIFRDLGVPIEVVDARDEFFAALKGI
                     TDPEEKREAITQTFYRQVFGRIVRESGAKYLLQGTILTDVDETVAGIKRQHNVFEQLG
                     IDPQEAFGYAILEPLIQLRKDGVRKVGKALGLPEEIFQRIPFPGPALAARIIGEVTPE
                     RVETVRKATAVVERLLAGTGAFQYLAVLHEDRVTGIRDGRREFGQQIEVRCWDSVDAR
                     RATPTRLPFDLLERLAQEILAEVPGVVSVTYNIATKPPSTIEAI"
     gene            6410..>6592
                     /locus_tag="PLY56_03770"
     CDS             6410..>6592
                     /locus_tag="PLY56_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20627.1"
                     /translation="MKTLRIFALFIALMLVMVAAHAWKPATDTAGSLTLTIPEIKEVT
                     ALEQPVPVVIRLENKGP"
BASE COUNT         1102 a         2135 c         2312 g         1033 t
ORIGIN      
        1 cgccagcagc aaggatctgt tcgagcggtt catcgtctgc tttggatagg aggtgtttgc
       61 gcgggctgtt ccatggcgcg gtgaacgctt gtcacggggc ggcgcgccag cccactcgcc
      121 atctccaaag gagggtgtca tgggatcgca ggccatcgtc acgcggattg cgctgatcgt
      181 gatgctcgtc gcgggaggcg cctggatcgc gcccggcacg ggagcaggag ccgctcccga
      241 cccggacctg ctggggcact gggcgtttga tgagggcgcg ggccccgacg cgatggagag
      301 tagcgggcgc gggcccgagg gggagatcca cggcgccgaa tgggtgaaag ggcggtttgg
      361 cacggcgctc cgcttcggcg ggagcggtgc ctacgtcacc atccctgaac tccgcggcct
      421 cgacggcagc gacgagatga cgctggaggc gtgggtctat tgggaggcga cggggcgcta
      481 tccgaacatc gtctcgggag gccgnnnnnn nnnnggcttc ctcctctttg tctcggacga
      541 ccactgtagc ttccgcatgg gcaagccggg gaaggagccg tttgaactgg gccgcgactg
      601 ggcggagacg ggtgcgaaca tcgggcggat tgtcctcggc cggtggcgcc acctggccgc
      661 gacgttcaag cgcccgaaac tcaccaccta cctggacggc gagccggttg gaagcgcaac
      721 gtgggactac ccagtgggct tctcgggcga gctgcggatt ggatgctgga gcagcccaaa
      781 cgtgtgccac accgggctga tcgacgaggt gaagatctac cggcgcgcgc tcacccccga
      841 agagatccgc gcgtcctacc tgaaagaggc gccgcgccgg caggtggccg agggcgaggc
      901 cccctacgag cggatccccg tggaggcaac acgactcgag ccggcggtga cgctcgagac
      961 gcggtgcgcg cgcctgtggc ttgaccggaa ggcgcgcgtc atcagcctgg cggataaggc
     1021 ggccagcaag gagtatcttg ccgacgcggt tcccatcgcc cggttgcgga ccgccgagag
     1081 cgagctgcgg cctacggcgt gttcttaccg cgacggcctc ctgacgctgg agtttggcca
     1141 gggcgaggcg actgccacgg tgaaggtgac accgcgcgac cactacctgg tttttgaagt
     1201 cgcttcgacg agcggcgccg cggccgagga gctgattttc ctcgacctgc gcgtgaagcc
     1261 gatgccgtat acgggcaatg gatccgggct gacggcggat gacgagttcg cgatctgcct
     1321 gcgcgcgctc aacctcctcg cccagcccgt gtttgggggg acgccgcgcc ggatgagcgc
     1381 cacgtgctct cgcgagcgcg gtatcattgg cgcccgggcg gcgctcgcgg cctgcccgac
     1441 gccgcagatc cggaagacgc tccaagagat gacgcggcgc gaggggctgc cccactcgcc
     1501 gctcggaggc gccttcgccc tggatgcccc ggagaaccgc tactcctacc tcttcacccg
     1561 caacatctcg gaggagaacg tggatctctg gatcctcatg gccaggcgcg ccggcatccc
     1621 ctacatccac ttctccgggt ggcagaagac gctggggagc tacgagccga ggccgacgct
     1681 cttccccaac ggcttcgccg ggctgaaggc gaccatcgac aagattcacg ccgccgggct
     1741 gaaggcgagc acgcacacgc tcaccgggtg catctcaccc accgacccgt tcgcgtcgcc
     1801 cgtgcccgac ccacacctgg cggtgcgttc cacgctgaca ctcgcggaag acgtggacga
     1861 gaaggcgacc acgatcccgc tggccgagtc tccggccggg atggacacga tctgggccta
     1921 cgcgagcaag gggaacgccg tgcgcctggg taacgaagtg gtgcagttta ccggagtgcg
     1981 cgaggagccg ccctacgcgc tgaccgggtg ccagcgcggc gcgtttggaa cgaagcccgc
     2041 cgcgcacaag aagggcgcgc cggtgagcta cctctacagc tggtacggca ccttctatcc
     2101 ggaccctgac tcgcccctgg cagaacgcgt ggccgactcc atcgcgcgtg ccatcaacga
     2161 gtgcgggttc gatatgatct acatggatgg cgccgagggg atgcctggcg cgaggggcta
     2221 tggcgccgcc aagatgcgca acacgatctt cagcaagatc cgccggcccg tgcgcgttga
     2281 ggcgagcaac ggcccggcgc accactcgtg gacgatgcac tcgtgcatgg gcgcgtggga
     2341 cctccctcac tgggggatca agccgtttat cgacctccac tgcaaggcga acgagagcgc
     2401 gcgtaccggc gagctaatgc cggcgcagtt ggggtggtgg gggatcctgg gtcccaccga
     2461 ggactacgat gccctcctgc cggacgagat cgagtatctc tgcgccaagg cgctcggctg
     2521 ggaccaggca ctctccttcc agggagtcat cccctcccag cgcccggtga acgcgcgcca
     2581 ggatgagtac ctgacgctga ttgggcgtta cgagcgcctg cgcctggccg actacttctc
     2641 cgaggatgtg cgcgcgaagc tgcgcgagcc gggcgccgag tatcacctgg tccaatcgcc
     2701 cgagggcgag tggtacttcc tgcccaggga ctacgcctcg cggaaggtga cccggggcga
     2761 ggagtggcgg gtgacgaacc gctacgcccg gcaaacgcca aagctgcgtg tccaggcgct
     2821 ctacgccgcc ggaccgtatg acggccagag cgtgaccctg gccgacttca cgcgcccggg
     2881 tgaattcggc gagccggccg ccgcggaggg cgttacgatc tcgctggagc cgtccacgga
     2941 gcaggtaaaa gccggcacgg cgagtggcaa gctcgtcgcc accaaccggg gcaaggaagc
     3001 gcgcggcgcg tgggcgcgcg tgacgcgtac cttctcgccg ccgctcgatc tgcggcccta
     3061 cggcggcttg ggcgtgtgga tctacggcga tggcaagggc gaggtgctca acttccagct
     3121 cacgaacatc cgccagttct gggagggcgg gatgacgtgc agcgagcact acgtgacggt
     3181 cgatttcacc ggttggcggt atttcgagct cctcttccgc gagcgagacg ccgaccgcca
     3241 tcgcgactac gcgtggccgt acaacccgct ttacgggatc taccgggcga ccctgcgccg
     3301 ccagtacatg ccggcgctca acctctacgt gaacaacgtg cccccgggcg agacgattac
     3361 ctgcttcatc agcccgatca aggcgcttcc ggtttcgaag gtgaagatcg cgaacccggc
     3421 ggtcaccctc ggtgggcagc gcgtcctctt cccggtgacg ctggaaagcg ggcagtcgat
     3481 tgagatggag tctcctgcgg agtgcaggct cttggacgac cgcggcgcac tggtcgagcg
     3541 cttcgcgcct cgtggcgcgg tgccggcgct ttcgcccggc gagaatcgcg ttgccttcga
     3601 gtgcgatgcg gtggaaggct accggccccg ggcgcgggtc accgtgatca ccgaggggcc
     3661 gccgctgcgc gggcgcgcgc cgcgcgagaa ggtggacgag cgctggctgc gcgaggagta
     3721 tgacgatcca cgcctgatcc gggcgattga cgggaaggag aacacgtggg agatctggtg
     3781 ccgccaggga acgaggggag ccagcctcga ggcggagatc gtggtcgagc gcctgtcgcc
     3841 cagccaggcg ggccagagca ccgagggcgc gcgcctcatc gaggggttcg aggatctggc
     3901 acgctttgcc gattcgccag agaaccagtt cgccaaatat gcctgggacg cggagcatca
     3961 gggcgtgcct gccaagccgg gcgttgcgca aagcctggag ctcgcgacag acgtggtgaa
     4021 agccggaaag gcgagcgcgc gcttcactgc gacgagcacg cgctcggacc atgccgggtg
     4081 gtgtgcccgg ggcgcgcgct tctcgccgcc gcttgacctc agcgggtatg ctgcggtcgg
     4141 gtggtggatc cacggcgacg ctggtggcca agcactgaaa ctacaatggc gcgatacggc
     4201 gggccggtgg ctggacctgg tgacgccggt cgatttccag ggctggcgct acgtggagtt
     4261 cccgctcgag gcgcgcgacg gcttcgacct ctcgcgcgtg gagtacctga tcctctactt
     4321 caacgggatt cccgcgggga agcgcgtcac ctgccatgtg gatgagctcc gcgtcctccc
     4381 gcggccggcg ggcctgcgcg agccccagct gacggtggat ggtcggacgg tgcgcttccc
     4441 ggtggcacta tcggcgggcg agcgcctggt gctggacgcg tccggccggg cccgccacta
     4501 cgcctctgat ggtcggctgg tggaggtcgt gaagccttcc ggcagcctgc cccttctgag
     4561 gccgggccgg caccagatcg ctttcggctg gggcgataaa ccagccggcg acttccaggt
     4621 gaaggtgatg gcggtgaaaa tctaccggtg acggcctcct ccgggtcggt cgggatagag
     4681 ggatagagtg cgagggagag gcgctgtggg ccagtccgtg ggcgataccc gcgcgcggag
     4741 ctggaagctc cgcgctgcct ttcggcaaag ccccttgcgg ggcttcccct cccaggcccc
     4801 gccaggcccg gttgggtgcg gaaggggcgt acacgttcgc tctggacacg gtcgtcttgc
     4861 ttgggttcgc cgccctgatc atgggtgcct aagcgctctc acaacggaga ccgccatccg
     4921 ccttctagcc ggacgcagtt tcgattggct gggaagacag agagcaccag cccgataggg
     4981 tttcgctcgc aatcagcgcg gagcttccag ctccgcgcgt tcggcccgcg cgccgccggc
     5041 cgggatcgtt atcccgatcg tttttccggc agagaaaacg caggaacgcg gacgtgtctg
     5101 gcgaaacgat tcctcccata agcaggagga ttgcgtccag gagtaggcgg tcgagagcgg
     5161 cacctgggta atctccaagc gcggggcgct cgcggttgca cgggtccggc atggcgccgg
     5221 gcccgcgttg ttgatagtca tgaagggaag agagatagtt cgatgttgaa ggagatcacg
     5281 gtccaggagc tggataccga tcagttcatt gccgaaaagg tccgcgaaat ccaggaagcg
     5341 gtgggcgatg gcaccgcgat caacgcgctc tcgggcggcg tcgattcgtc cgtggtgacg
     5401 atgctcgggc accgcgccct tggaaaccgc ctccgcacgg tcttcgtcga gaacgggatc
     5461 atgcgcgagg gcgaggcgga gcggatcgag cggatcttcc gcgacctggg cgtgccgatc
     5521 gaagtcgtcg acgcgcgcga cgagttcttc gccgcgctca aagggatcac cgaccccgag
     5581 gagaagcgcg aggcgatcac gcagacgttc taccgccagg tgttcgggcg aatcgttcgc
     5641 gagagcggtg cgaagtactt gctccagggc acgatcctca ccgacgtgga tgagaccgtg
     5701 gccgggatca agcggcagca caacgtcttc gagcaactcg ggatcgaccc gcaagaggcg
     5761 tttggctacg ctatcctcga gccgctcatc cagctccgca aagacggcgt gcgcaaggtc
     5821 ggcaaggcgc tcgggcttcc cgaggagatc ttccagcgga tccccttccc cgggccggcg
     5881 ctcgcggcgc gcatcatcgg cgaggtgacg cccgagcgcg tcgagaccgt gcgcaaggcg
     5941 actgcagtcg tggaacgcct cctcgcgggc accggcgcct tccagtatct cgccgtgctc
     6001 catgaggacc gcgtcactgg aatccgcgac ggccgacgcg aattcggcca gcagatcgag
     6061 gtccgctgct gggatagcgt cgatgcccgg cgcgccacgc ccacccggct tccgttcgac
     6121 ctcctggaga ggctagcgca agagatcctg gccgaggtcc ccggcgtggt cagcgtcacc
     6181 tacaacatcg ccacgaaacc cccctccacg atcgaagcga tctaaacccg cgcggccggc
     6241 ccaggcgatc cgagcccggg ccggccgctc tcccatctct accaccggcc ttgcccgaga
     6301 aaggggtttg aaggcacccg ccgaaaccca ttcctgaggg atggcccccg cgctctcggt
     6361 ccagcgagct ctccccaggg aaatggccat ccagaggggg gctgacgaga tgaagacgct
     6421 tcgcatcttc gcgctgttca tcgccctgat gctggtcatg gtggctgcgc acgcctggaa
     6481 gccggccacc gataccgccg gctcgctcac gctgacgatt cccgagatca aagaagtgac
     6541 ggcgctggag cagcccgtgc cggtggtgat ccgcctggag aacaaaggcc cc
//
LOCUS       DAOSCV010000100         6589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7475_length_6589_cov_4.097643, whole genome shotgun sequence.
ACCESSION   DAOSCV010000100 DAOSCV010000000
VERSION     DAOSCV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7475_length_6589_cov_4.097643"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PLY56_03775"
     CDS             complement(<1..966)
                     /locus_tag="PLY56_03775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ20628.1"
                     /translation="MIDQKRVRETFLRLLRMNTPSGQEGPVADFLEAELRALGFETWR
                     DGAGKAIGGETGNLLARRPGSAPEAPTLLLNAHMDTVAPTEALEIVEEPDGVIRSSGT
                     TILGADDKAGLTAILEGVRAAVAEGFPMPTVEIAFTIAEETGLRGARHLDYSSLTATQ
                     GYAFDGGKPVGGIIVAAPSQDNVTAVVHGVAAHAGAAPEKGVSAIRIAAEAITRMPLG
                     RIDDETTANIGVIHGGHATNIVPDRVECRGEARSHNDEKLARQTRAMVDAFNQAAAAA
                     GGRADVRVERVYSHFGFSESDPLVRRAVTAGSRAGGAHCWCVDSRN"
     gene            complement(979..1956)
                     /locus_tag="PLY56_03780"
     CDS             complement(979..1956)
                     /locus_tag="PLY56_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20629.1"
                     /translation="MGRTLSVGIIGAGAIAAARHAPGYERAPGAEILAVADANLDRAE
                     VLAKKHGVPIVYQDYRELLRRTDIDAVSICTPNALHAEHALAAIQAGKHVLCELPIAL
                     GLGECRELIQMARAHGVLLMPTLAERFSAAHRQAREIVASGQIGRPLFFRASFASPRS
                     PAASDWRVDPALSGGGALIDLGLHALDLFRFHMGAVSTVLADTGTLAGEAPVEDAAVC
                     VVHGDGARGCIEARWCGAVPARSVEIHGTAGGVLVDAGKPVHVCGAGTGWEPVAAPST
                     EGPAGIVAHFVACCQQAAEPEVAAEEGTRALELVLAAYDSARRGQPRQI"
     gene            complement(1991..2950)
                     /gene="prmC"
                     /locus_tag="PLY56_03785"
     CDS             complement(1991..2950)
                     /gene="prmC"
                     /locus_tag="PLY56_03785"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ20630.1"
                     /translation="MSAPAEQRGVVERTVAQALREATAALAAAGIERARVEAEILLAH
                     VLRARRIDLVASPERVLTGQEAEAFDRLVARRATRYPLPYLTGEREFMGLRFHVNEHV
                     LIPRPETEVMVEAAIEALRGWVAAEGECSQDPELGAPVAIDVGTGSGCIAISLAHFVP
                     QARVLATDISPEACAVARLNAARLGVASRVTVYAGDLLEPLEEVTGAKVPLARRAAVV
                     CANLPYIPEENLGKLQPEVRDWEPAIALGGGPGGLHVVERFLEQVQSHLRAGGTLFLE
                     IGPEADQAPRLTAALGARGRWRDVRVLRDLAGWPRVVAAIYAA"
     gene            complement(2947..4035)
                     /gene="prfA"
                     /locus_tag="PLY56_03790"
     CDS             complement(2947..4035)
                     /gene="prfA"
                     /locus_tag="PLY56_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ20631.1"
                     /translation="MLRRLQEIEERYEVLEQRLADPALLGNPQEYREIAKQHASLAEI
                     VGLHRQSEEVARQIEEVRALLEDPEMREMAQAELETLRAQAADLEQQIRIALLPRDPA
                     DEKNVFLEIRAGEGGEEAALWAADLARMYTRYAERHGWKVEMVSSNPTGIGGFKEVIL
                     LLKGQGAYSRLKFESGGHRVQRVPVTESQGRIHTSSATVAVMPEVEEVEVQIDPADLE
                     IETYRSSSAGGQNVQKNETAVRITHKPTGIVSSCQDERSQHQNKEKAMNMLRSLLYTR
                     MKEEQEAQQRQARRSQVGSGERGDKIRTYNFPQNRVTDHRLGGSAGTIHGMEQVLDGD
                     IDRLIERLIESDQAERLARLDESDGSES"
     gene            complement(4010..>4422)
                     /locus_tag="PLY56_03795"
     CDS             complement(4010..>4422)
                     /locus_tag="PLY56_03795"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ20632.1"
                     /translation="HGAEHQVIHAFEKGDAVSPETASAYDTAHPRCGTSFIAVVVVVS
                     ILAFSFIDVSTWYVRQALKLVLLPVVGGISYEVIRLAGAGKLTFLVTPGMWLQRLTTR
                     QPTADQVEVAIAALEGVLAQEGRSLNRAAEATGD"
     assembly_gap    4423..4432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4433..5083)
                     /locus_tag="PLY56_03800"
     CDS             complement(<4433..5083)
                     /locus_tag="PLY56_03800"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ20633.1"
                     /translation="MFSLLPFLASKPGEACPQASYGGQAVLEGVMIRSPQALAVAVRR
                     LDGRIVLKRESLVPLTKRHPLLNVPFIRGAFALADALITGMRALSFSADVAMEEENER
                     AAAAAQQQAGGGEGERPEDDSRVPPAPGENRVEVRDVAAGKGIPWWSLALTLMASLGI
                     VILFFVMLPDAVANLMRGRLPLGAASNVVINLAEGLLRVGLFIAYIVAISRMEAIRR"
     gene            complement(5151..5369)
                     /gene="rpmE"
                     /locus_tag="PLY56_03805"
     CDS             complement(5151..5369)
                     /gene="rpmE"
                     /locus_tag="PLY56_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOJ20634.1"
                     /translation="MKQGIHPKYQEATVSCACGNKFTTRSTKPDIHVEICSVCHPFFT
                     GTQKIVDTEGRVEKFLAKYGMQAEKKQS"
     gene            complement(5955..>6589)
                     /locus_tag="PLY56_03810"
     CDS             complement(5955..>6589)
                     /locus_tag="PLY56_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20635.1"
                     /translation="LALLVAAPTTLTGITIAHASPSGLNNIPTADVAPTGVPVVQVYT
                     TLAEGNSSHAILGVKYGATSRVEIGVDSGVGKRYSPAFQAKLQVPLRPGLPDLALGVA
                     NLSTNTARDGEPAWYAVVSTKGRLRGHAGHILQANNPALFVGADYAASQSLMLRADWI
                     QTNDREDSTASLGFMRSFGRRVVFEGWASFPSASGAKTAYTIKLNYVLGP"
BASE COUNT         1097 a         2355 c         2025 g         1102 t
ORIGIN      
        1 atttctggaa tcgacacacc agcaatgggc tcctcctgca cggcttccgg cagtgaccgc
       61 gcggcgtacc aggggatcac tctccgagaa tccgaagtgg gagtagactc gctccacacg
      121 cacatcggcg cgcccgccgg ccgccgctgc cgcctggtta aaggcatcga ccatcgcccg
      181 tgtctggcgg gccagcttct cgtcgttgtg gctgcgcgcc tctccccggc actccacgcg
      241 atccggcacg atgttcgtgg cgtgcccacc gtgaataacg ccgatgttag ccgtcgtctc
      301 atcatcaatg cgccccaggg gcattcgggt gatcgcctcc gcggcgatgc ggatcgcgct
      361 cactcccttc tcgggcgccg cccccgcgtg cgccgccacg ccatgcacga cggcggtcac
      421 gttatcctga gagggagcgg ccacgatgat gcctcctacg ggctttcccc cgtcgaacgc
      481 atacccctgg gtcgcagtca gcgaggagta atcgaggtgc cgtgccccgc gcaggcccgt
      541 ctcctcggcg atggtgaagg cgatctccac cgtgggcatc gggaagcctt ctgccacggc
      601 ggcacgcact ccttcgagga tcgccgtcag ccccgcctta tcatccgcgc ccaggatcgt
      661 cgttcccgag gagcggatca cgccatccgg ctcttccacg atctccagcg cctcggtcgg
      721 ggccacggta tccatgtgtg cgttgaggag caaggtggga gcttccgggg ccgatcccgg
      781 ccggcgcgcc aggagattgc ccgtctcgcc cccgatcgcc ttgccggcac cgtcacgcca
      841 tgtctcaaag cccagcgcgc ggagctcggc ttccaggaaa tcggcgaccg ggccttcctg
      901 gcccgaagga gtgttcatcc gtaacagccg caggaacgtc tctcgcacac gcttctggtc
      961 gatcattgcg ctcccccttc aaatctgcct cggctgcccc cggcgggccg agtcgtacgc
     1021 ggcaaggacg agctccaacg cccgcgtgcc ctcttcggca gccacttccg gctccgctgc
     1081 ctgctggcag caggccacaa aatgggccac gatgccggca gggccctcgg tggatggcgc
     1141 tgccaccggc tcccatcccg tcccggcgcc gcacacgtgc accggctttc cagcatccac
     1201 gagcactccc cccgcggtgc catgtatctc cacgctccgc gccgggaccg cgccgcacca
     1261 gcgcgcctca atgcaccccc tggcgccgtc gccgtgcacc acgcacaccg ccgcatcctc
     1321 cacgggcgcc tcgcccgcca gtgtgcccgt gtcggccaga acggtagaga ccgcgcccat
     1381 atggaagcgg aagaggtcca gggcatgcag ccccaggtcg atcagggcgc caccgcccga
     1441 gagcgccggg tcgacgcgcc aatcgctcgc cgcaggggat cgggggctcg caaagctagc
     1501 gcggaagaag agcgggcgcc caatctgccc ggaggccacg atctcgcgcg cctgccggtg
     1561 cgcagcggag aagcgctcgg ccagggtcgg catcaacaga acgccgtgcg cccgcgccat
     1621 ctggatcaac tcgcggcact cgcccaaccc cagggcgata ggaagctcgc acaagacatg
     1681 cttccccgct tggatcgcgg ccagggcgtg ctcggcatgg agggcgttgg gggtgcagat
     1741 gctgaccgcg tcgatatcgg ttcgtcggag taactcgcgg tagtcctggt agacaatcgg
     1801 cacgccgtgc ttcttggcaa gcacctcggc gcgatccagg ttggcgtcgg ccacggccag
     1861 gatctcggcg ccgggcgcgc gctcatatcc gggcgcatgg cgcgccgcgg caatcgcccc
     1921 tgcgccgatg atgccgacgc tcaacgtcct tcccatacct gtctccccgc gagccagccg
     1981 gactggcggc tcaggcggcg tagatggccg ccaccactcg tggccagccc gccagatctc
     2041 gtaacacccg gacatcgcgc cacctgccgc gcgcacccag agcggcggtc aggcgcggcg
     2101 cctggtccgc ctcggggcct atctccagga agagagttcc gccggcacgc agatggctct
     2161 gcacctgctc caggaagcgt tccaccacat ggaggccgcc cggaccgccc cccagagcga
     2221 tcgcaggctc ccaatcgcgg acttccggct gcagcttccc caggttctcc tctgggatgt
     2281 atggcagatt ggcgcagaca accgccgccc ggcgagccag cggcacctta gcgccggtca
     2341 cctcttcgag aggctccagg agatctcctg catagaccgt cacgcgactg gccaccccca
     2401 ggcgcgcggc gttcagccgg gccaccgcgc acgcctccgg cgagatatcc gtcgccagca
     2461 cgcgcgcctg gggcacgaag tgcgccagac tgatcgcgat gcaccccgaa ccggtgccga
     2521 catcaatcgc cactggcgcg cccagctccg ggtcctggga gcactcccct tccgcagcca
     2581 cccaaccgcg gagggcctcg atcgccgcct cgaccatcac ctcggtctcg gggcggggaa
     2641 taaggacgtg ctcgttgacg tgaaagcgga ggcccatgaa ctcgcgctcg cccgtgaggt
     2701 agggcagcgg gtagcgcgtc gcccgacgcg cgacaaggcg atcaaacgcc tctgcttcct
     2761 gaccggtgag cacgcgctct ggagaggcga ctaggtcgat ccggcgcgct cgcagcacat
     2821 gcgcgaggag gatctccgct tccactcgcg cgcgctcgat tccggcagcg gcgagggccg
     2881 ccgtggcctc gcggagcgcc tgcgccaccg tgcgctccac gacgcctcgc tgttcggccg
     2941 gcgcgctcac gactccgacc catcggactc atcgaggcgc gcgagccgct ccgcctggtc
     3001 cgattcaatc agccgctcga tcaaccggtc gatatcgcca tcgagcacct gctccatgcc
     3061 atgaatggtg ccggcgctgc cgccgagacg gtggtcggtg acccggtttt gcgggaagtt
     3121 gtaagtgcga atcttgtcgc cgcgctcgcc cgagccaacc tgactgcgcc gcgcctgccg
     3181 ctgctgcgcc tcctgctcct ccttcatgcg ggtgtagagg agagagcgca gcatgttcat
     3241 cgccttctct ttgttctggt gctgagagcg ctcgtcctgg cagctcgaga caatccccgt
     3301 tggcttgtgg gtgatgcgca cagccgtctc gttcttctgg acgttctgac cgcccgccga
     3361 agaagagcga tacgtttcga tctcgagatc cgccggatcg atctgcactt ccacttcctc
     3421 cacctccggc atcaccgcca cggtggcgga agaggtgtgg attcgcccct gcgactcggt
     3481 aacgggcacg cgctgcacac ggtgccctcc gctctcgaac ttcaagcgcg agtaagcacc
     3541 ctggcctttg agcagaagga tgacctcctt gaaccccccg atgcccgttg gattgctgga
     3601 gaccatctcc accttccagc cgtgccgctc ggcgtagcgg gtgtacatgc gcgccaggtc
     3661 ggctgcccac agggcggctt cctcgccgcc ctcgccggcg cggatctcca ggaagacgtt
     3721 cttctcatct gccggatcgc gcgggaggag ggcgatccgg atttgctgct ccagatcggc
     3781 ggcctgagcg cggagcgtct ccagctcggc ctgcgccatc tcgcgcatct ccgggtcctc
     3841 cagcaaggcc cggacttcct cgatctggcg tgctacctct tcgctctggc ggtgaaggcc
     3901 aacgatctcc gcgagggagg cgtgctgctt cgcgatctcg cgatactcct gggggttgcc
     3961 cagcaacgcg ggatcggcca ggcgctgttc cagcacctca taacgctcct caatctcctg
     4021 tagcctccgc agcacggttc aacgaccttc cttcctgcgc caaaacgccc tccagggcag
     4081 caatcgccac ttccacctgg tcggcagtgg gctggcgggt ggtcaaccgc tgcagccaca
     4141 ttcccggggt caccaggaag gtgagcttgc ccgcgcccgc caggcggatc acctcgtagg
     4201 agatgccgcc caccaccggc aagagcacca gtttcagcgc ctggcgcaca taccaggtgg
     4261 acacatcaat aaaactaaat gccagtatgc tgaccacgac cacaaccgcg atgaagctcg
     4321 tgccacaacg cgggtgggcc gtatcgtaag ccgaagccgt ctccggcgag accgcgtcgc
     4381 ccttctcgaa cgcgtggatc acctggtgct ccgcgccatg atnnnnnnnn nnccgccgga
     4441 tcgcctccat tcgcgagatg gcgacgatgt aggcgatgaa gagccccacg cgcagcagcc
     4501 cctctgccag gttgatcacg acatttgacg cagcccccaa aggcaggcgc ccccgcatca
     4561 agttcgccac cgcgtccggc agcatgacga aaaagaggat cacaatcccc aggctcgcca
     4621 ttagcgtcag cgccagcgac caccacggga tccccttgcc ggcggccacg tcgcgcacct
     4681 ccacccggtt ctctccgggg gccggtggca cgcgggagtc gtcctccggg cgctcgccct
     4741 caccgccgcc ggcctgctgt tgcgcggctg cagcggcgcg ctcattctcc tcctccatgg
     4801 ccacatcggc ggagaaagag agggcgcgca ttcccgtgat cagcgcatcc gccagcgcga
     4861 acgcgccgcg gatgaacggc acattgagga gcggatgccg cttcgtcaac ggcaccagcg
     4921 actcccgttt cagaacaatc cggccgtcca agcggcgcac ggcgaccgcc agcgcctgcg
     4981 gggagcggat catcaccccc tcaagcaccg cctgaccgcc atagcttgcc tgcgggcacg
     5041 cctcgcccgg cttactcgcc agaaacggca agagggaaaa cacggacagc cgccgcgagc
     5101 gtctcggacg gggccggtct tccctctttt ccatacctga cgatgggatg ctacgactgc
     5161 ttcttttcgg cttgcattcc atacttagcc aggaacttct ccactcgccc ttccgtatcg
     5221 acgatcttct gggtgccggt gaagaacggg tggcacaccg agcaaatctc aacgtgaatg
     5281 tccggcttcg tcgaccgggt ggtgaacttg ttcccacaag cgcagctcac cgtagcctct
     5341 tgatacttgg gatggatccc ctgcttcatc ttcttagccc tcgccaatac tcgcgcccga
     5401 tgccctcctg caactttggg gcctcttggg cgatgcaaac acaatgcggc gacagccgat
     5461 ccccgaaggg ggcacgcgct gccgccggcg tgtctagccg ccaacgcttc tttacgcgat
     5521 gagtattata gcacacaacg cgactcctgg caaatccatg cgacggagca ctgggattcc
     5581 gacataagga gaaggccgcg cgcacgcgcg gagcttcgag ctccgcgctg ctttccggca
     5641 aagcccttcg gggcttctcc tcccagccac cgccgttttg agacatctgg gagtatcccc
     5701 aacccagccc ggcagggctt cgctcgcaag cagcgcggtg atttatcgcc gcgcgctggg
     5761 ttgcgcgctg cctccgaggc ttcggattcc ccatctcgga atctcaggac agatcactgg
     5821 gattccggga tagggaatcc gaagccgggc gtaggaacca ccggctcgaa tgccggtgct
     5881 gttcggccat agttgccttc agttctccgg cgtgatatac ataacagttc atcgcagttg
     5941 cctccggtcg gtcactatgg ccccagaacg taattgagct taatggtgta ggcggtcttg
     6001 gcgccgctcg cggaggggaa ggaggcccat ccctcaaaga ccacacggcg cccaaaggaa
     6061 cgcatgaacc cgaggctcgc tgtggagtcc tcccggtcgt tagtctggat ccagtccgcg
     6121 cgaagcatca acgactgaga ggcggcgtaa tctgcaccca cgaagagtgc cggattgttg
     6181 gcctgaagta tgtggcccgc gtgaccgcgc aggcggccct tggtgctgac cacggcatac
     6241 caggctggct cgccgtctcg cgccgtgttc gtcgacagat tggcaacgcc gagggcgagg
     6301 tcaggcaacc ccgggcgcag gggcacctgg agcttggcct ggaaggcagg gctgtaccgc
     6361 ttgccgactc cactgtcaac gccgatctcc acgcgggagg tcgcgccgta cttcacgccc
     6421 aggatggcat gcgacgagtt gccctcggcg agcgtcgtgt agacctgcac gacgggcacg
     6481 ccggtggggg ccacatcggc cgtggggatg ttgttcaggc ccgatggcga ggcatgggcg
     6541 atggtgatac ctgtcagtgt cgttggcgcg gccaccagga gagccagcc
//
LOCUS       DAOSCV010000101         6584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7484_length_6584_cov_3.715423, whole genome shotgun sequence.
ACCESSION   DAOSCV010000101 DAOSCV010000000
VERSION     DAOSCV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_6584_cov_3.715423"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..953
                     /locus_tag="PLY56_03815"
     CDS             153..953
                     /locus_tag="PLY56_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20636.1"
                     /translation="MALPQDRQRAEVGAPAPPAPEFQPAPLALRVRGLRYRYPDGTPA
                     LDGVNLEIAPGEAVALIGPNGAGKSTLLLHLVGILHGEGEIEAFGLKPAGPELKMLRQ
                     KVGLLFEDPDDQLFMPRVRDDVGFGPRNLGLPPEEVNRRIEQALAATGMTGTEERAPH
                     HLSSGQKKRVALAALLAMEPELLLLDEPTSTLDPRARRELIRVLEALPVAKLIATHDL
                     ELVLELCPRAVILDRGRVVADGPSAALLSNAELMEAHGLEVPGSLHPR"
     gene            complement(978..2858)
                     /locus_tag="PLY56_03820"
     CDS             complement(978..2858)
                     /locus_tag="PLY56_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HOJ20637.1"
                     /translation="MSALQPMALLWLLLAVPAILLLYLLKPKRREHLISSVLLWDRLL
                     KDTQARTPFQKLRRSLLLLLQLLLVVLLAVALSRPFVRIHAFGGQSMAIVIDGSASMR
                     ATDVPGSRFEEARRVARRMIEGMGQSDRAVLILAGARAHAACPLTGDKNALCRALDEI
                     RPADTETRMREAVLLAASLLAGQPDARIYLLTDGGFEMEEGEGAQAGLPLGEAQLHFV
                     RIGQKGDNVGVTALDVRQTLADDGGYQAFARVRNFSDQARRFTLEFYRDDRLLDAREM
                     TLGPGKAHAEVFALSRDAATGVLRARIDAADDLAVDNSAAVVLQPRKRVEVLLVSRGN
                     LFLEKALSVDPRVALSKIAPGPDAAVAAPRYDLVILDGKSPAGLPARGRYLFIGGAGE
                     HAPVTITGETRNPTFLDWDRRHPVTRFLDFSSVQVARALAAKPLPWGQTLAECEAGAL
                     IAAGEKDGLRSLFLAFDLTESDLPLRVAFPILLSNAVEWLMGSEEAASSHRARMGAVV
                     PVEVPEDAREVQVFLPDGSRRSLRVEGSPLLFAETAQTGIYRVEGKSQGKPFRIQFAV
                     SLLSEAESDIRPRDRIAIGRTSVPAVDARSQKAPREIWHGVAFLALCLMAFEWYAYHR
                     RL"
     gene            complement(2855..3769)
                     /locus_tag="PLY56_03825"
     CDS             complement(2855..3769)
                     /locus_tag="PLY56_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ20638.1"
                     /translation="MAETRPTTPPGKLGASLLEPGFLAKLERLALVSRRVYAGQMKGE
                     RRSTRRGGSVEFADFRDYVPGDDLRQVDWNTFARLDRLFLKLFVEEEDLTVHLLIDGS
                     RSMAFGSPPKGDYARRVAAALGFIALFGFDRVTASVLAEERVYTLPAVRGRPQVFQLF
                     RFLEEARFEGRTNLARSLRDYALQSARPGLAVLLSDCFDPGYAEGLKALAARRFDVAV
                     LHLLDPTEVAPDLTGDLKLVDAETGEIREISVSQHLLGQYRKALEEFCAGLKQVCSRY
                     GMEYIRATTDVPFEELVLRVLRGAGVVR"
     gene            complement(3762..4967)
                     /locus_tag="PLY56_03830"
     CDS             complement(3762..4967)
                     /locus_tag="PLY56_03830"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ20639.1"
                     /translation="MLIPIPYATDRELRHFPIVTLFLVGLNCSLFLLSLMGGTLTLDY
                     IVQNYGLRGDAPEIATLFSSMLLHASALHLLVNVTYLWLFGCLVEDVLGRVEYMLFYL
                     GSGMAGGLLYVVVVQKVLPAVAATPLVTPEPAVGASGAVAGILGLFAIRFYRAKIKML
                     LLVPIAIPALPAIGFWVGIQGACAVSSLSTGVNTGCWAHLGGFAFGILSGCLMRVVPV
                     AEEEYFFEDVERDLAENNVKSALRRLHEKQGSSPDDPKVHCLLAKAYEKSGSPDVADM
                     HARLSVELALEKGDLETAVANYLAVKGVLSVLAMEAQYRIACALESQRRTAEAAAAYR
                     RVYHYYPEEPLAGTALIRCVEMLRRRPEGAEEAAALAREYLERYPEGEWRAQAERYAR
                     SAPFRDGNG"
     gene            complement(4986..5999)
                     /locus_tag="PLY56_03835"
     CDS             complement(4986..5999)
                     /locus_tag="PLY56_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007338993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ20640.1"
                     /translation="MTPTTEIAARAEAYRERYAAVCDEIRKVIVGQEEVIDGVLTSVF
                     AGGHVLLEGVPGLGKTLLVRTLARVLHLKFSRIQFTPDLMPADITGTNIVTESPSGGR
                     QFLFQPGPIFANVVLADEINRATPKTQSALLEAMQEQSVTVAREVRRLEEPFFVLATQ
                     NPIEQEGTYPLPEAQVDRFFFKLLVGPPCLEELDAIIDRTTGTEEPELRVILDGPALL
                     EMRALCRAVPIAPHVKEWALKIVMATHPDSELAPPQVKRFVRYGSSPRGAQALVLAAK
                     TRALLDGRYNVSFDDIRRSALPALRHRVLLNFEGLAEGVQPDDLVQEILESLSPAGDG
                     EIG"
     gene            complement(6041..>6584)
                     /locus_tag="PLY56_03840"
     CDS             complement(6041..>6584)
                     /locus_tag="PLY56_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20641.1"
                     /translation="EAVMQSRQRLALAGQEAPQGGKEGLPSGQGAMARGDAHAGGAQG
                     EKAQGHGTHQLATQDGGSEAGRGVTNRQVAGGTMESGTPRDRQADHRSEMRARFEQVY
                     APHRVDTNRLDTQVRGKTRAGKSDYSEVRAAPDPGSASRPYYEVYSHYQRTAEEALAR
                     EEIPAGYRKQVRDYFDSLRP"
BASE COUNT         1121 a         2262 c         2203 g          998 t
ORIGIN      
        1 tttcatttca ttattctaat gaataatgcg gggcagccgc cgggcacagg ccgggcagga
       61 agcgtgctac tctccggcga accggtgcta tgccagacaa gctacggcgg ccaggtggcc
      121 gctaccgcgt ctgatgaaac cgcgcacatg ccatggcact tccccaggac aggcaaagag
      181 cagaggtcgg cgctccggcg ccgccggctc cggagttcca acccgcccca ctcgcgctcc
      241 gcgtgcgcgg cctccggtat cgctacccgg atggcactcc cgcgctcgac ggggtgaacc
      301 tggagatcgc cccgggagag gccgtggcgc tgatcgggcc gaacggcgcg gggaagtcga
      361 cgctcctgct ccacctcgtg ggcatcctgc atggcgaggg cgagatcgag gcgttcgggc
      421 tgaaacccgc gggtccggag ctcaagatgc tgcgccagaa ggtgggtctg ctttttgagg
      481 acccggacga ccagctcttc atgccgcgcg tgcgcgatga cgtcggcttc gggccgcgca
      541 acctggggct gccgccagag gaggtgaacc ggcgcatcga acaggccctc gcggccaccg
      601 gcatgacagg cacggaagag cgcgccccac accacctctc ctcggggcag aagaagcgcg
      661 tggcgctcgc ggccctgctc gcgatggagc cggagctgct cctcctggat gagccgacga
      721 gcacgctcga cccgcgcgcc cggcgcgagc tgatccgcgt cctcgaggcg ctgccagttg
      781 ccaaactgat cgccactcac gacctggagc tggtgctgga gctctgcccg cgcgccgtga
      841 tcctcgatcg cgggcgtgtc gtcgccgatg ggccatccgc agcgctgctt tcgaacgcgg
      901 agctgatgga ggcccacggg ctggaggtgc ccggcagcct gcatccccgg taagccgatg
      961 cctgacgatg cccgggccta cagccggcgg tgataggcat accactcgaa cgccatcagg
     1021 cagagcgcca ggaaggcgac cccgtgccag atctcacgtg gcgccttttg agaccgggca
     1081 tccacggcgg gtacgctcgt gcgcccgatg gcgatccggt cgcgcggccg gatatccgac
     1141 tccgcctcgc tcagcaggct gacggcaaac tggattcgga acggcttccc ctggctcttc
     1201 ccctccacgc ggtagattcc cgtctgcgcc gtctcggcga agaggagcgg ggatccctcc
     1261 acccgcagcg agcggcgcga gccgtctggg aggaagacct gcacttcgcg cgcgtcttcc
     1321 ggcacctcga cgggcaccac ggcgcccatg cgcgcgcgat ggctggaggc cgcctcctca
     1381 gagcccatca gccactcgac ggcgttcgag aggaggatcg ggaaggcaac gcgcaggggg
     1441 aggtcggatt ccgtcagatc gaacgcgaga aagagcgagc ggaggccgtc cttctcgcca
     1501 gcggcgatga gcgctccggc ttcgcattcg gcgagtgtct gaccccaggg gagcggtttg
     1561 gccgcaagcg cccgggccac ttgcacgctg ctgaagtcga gaaaccgcgt gacggggtgc
     1621 cggcggtccc aatcgaggaa agtgggattc cgggtttccc cggtgatcgt gaccggcgcg
     1681 tgctcgccgg cccccccgat gaagaggtag cgcccgcgcg cgggaaggcc ggcaggcgac
     1741 ttgccatcca ggatgacgag atcataccgg ggcgcggcca cggcggcatc tgggccgggc
     1801 gcgatcttgg agagcgccac acgcgggtca acgctcagcg ccttctccag gaagaggttg
     1861 ccccggctca ccaggaggac ctcgacgcgc ttgcgtggct ggagcaccac cgccgcgctg
     1921 ttatcgaccg cgagatcgtc ggccgcgtca attcgcgcgc gcaggacgcc cgtggcggcg
     1981 tctcgcgaga gtgcgaacac ctcggcatgg gcctttcctg gcccgagcgt catctcgcgc
     2041 gcgtcgagaa gccggtcatc gcggtagaac tccagggtga agcgccgcgc ctggtcggaa
     2101 aagttgcgca cgcgggcaaa cgcctggtaa ccgccatcgt ccgcgagcgt ctggcgcacg
     2161 tcaagcgcgg tcacgcccac gttgtcgcct ttctggccaa tgcgtacaaa gtgcagctgc
     2221 gcctcgccaa gcggcaatcc agcctgagcc ccctcccctt cctccatctc gaagccgcca
     2281 tcggtgagga ggtagatgcg cgcgtcgggc tgcccagcca ggagcgacgc cgccaggagc
     2341 acggcctcgc gcatccgagt ttcggtatcg gccgggcgga tctcatcaag ggcccggcac
     2401 agcgcgttct tatcgccggt gagggggcag gccgcgtggg cgcgcgcgcc ggccaggatc
     2461 agcacggccc tatcgctctg gcccatgcct tcgatcatgc gccgggccac gcggcgcgcc
     2521 tcttcaaacc ggctcccggg cacgtcggtc gcgcgcatgc tcgctgaccc gtcgatcaca
     2581 atcgccatgc tctggccacc aaaagcgtga atgcgcacga agggccggct gagtgccacc
     2641 gccagcaaca ccaccaggag aagctggagc aggagcaaga ggctccggcg gagcttctgg
     2701 aacggcgtgc gcgcctgtgt atccttcagc agccggtccc agaggaggac cgacgagatg
     2761 aggtgttccc gccgctttgg cttcagcagg tagagaagca ggatcgccgg cacggccaag
     2821 aggagccaga ggagggccat cggctggagc gcgctcaccg gaccaccccc gccccgcgca
     2881 gcacgcgcag caccagctcc tcgaatggaa cgtccgtagt cgcgcgaatg tactccatgc
     2941 cgtagcggga gcacacctgt ttcaaaccag cgcagaactc ctccagcgcc ttccgatact
     3001 gcccgaggag gtgttggctc acgctgatct cgcggatttc gcccgtttcg gcatccacca
     3061 gcttcagatc gcccgtgaga tcgggggcca cctcggtggg gtcgaggagg tggaggacgg
     3121 cgacatcgaa ccggcgcgcg gcgagcgcct tcaggccctc ggcatagccc gggtcgaagc
     3181 aatcggaaag gagcaccgcg agcccggggc gagcgctttg aagggcatag tcgcgcagcg
     3241 agcgcgccag gttggtccgc ccctcgaacc gggcctcctc caggaagcgg aacagctgaa
     3301 acacctgggg ccggccgcga acagccggca gggtgtacac cctctcctcg gcgagcacgg
     3361 acgcggtcac ccggtcgaag ccgaagagcg cgatgaagcc gagagcggcg gccacgcgcc
     3421 gggcatagtc gcccttcggg ggagagccga aggccatgga gcgagagcca tcgatgagga
     3481 ggtggacggt cagatcctcc tcctcgacaa acagcttgag gaagaggcga tccaggcggg
     3541 caaaggtgtt ccagtcaacc tggcggaggt cgtcgccggg cacgtagtca cggaaatcag
     3601 cgaactcgac cgagccgccc cggcgcgtgg agcggcgctc gcccttcatc tgcccggcat
     3661 agacgcgccg ggagacgagc gccaggcgct cgagcttcgc cagaaagcct ggctccagga
     3721 ggctggcgcc cagtttcccg ggcggtgttg tcggtctcgt ttcagccatt gccatcgcgg
     3781 aacggcgcgc ttctcgcgta gcgctcggcc tgcgcgcgcc actcaccctc ggggtagcgc
     3841 tccagatact cgcgcgccag cgccgctgct tcctccgcac cctccgggcg ccggcgcaac
     3901 atctccacgc agcggatcag cgccgttccg gcgagaggtt cctcgggata gtagtggtac
     3961 acccggcggt aggctgccgc ggcttcggcg gtccggcgct ggctttccag ggcgcaggcg
     4021 atccggtact gcgcctccat cgcgaggacg ctcagcaccc ccttcacggc aaggtagttc
     4081 gccaccgccg tctcgaggtc tcccttctcc agcgcaagct ccaccgagag gcgagcgtgc
     4141 atgtcggcca cgtcgggcga gccgctcttc tcgtaggcct tcgcgaggag gcaatggacc
     4201 tttggatcat ccgggctgct cccctgtttc tcatggaggc ggcgcagcgc gctcttgacg
     4261 ttgttctcgg ccaggtcgcg ctccacgtcc tcgaaaaagt actcttcttc cgccacaggc
     4321 accacgcgca tcagacagcc gctgagaatc ccgaaggcaa acccacccag gtgcgcccaa
     4381 caccccgtat tcacgcccgt agagagcgac gacacagcgc acgccccctg aatgcccacc
     4441 cagaacccaa tcgccgggag cgcgggaatg gcaatgggaa ccaagaggag catcttgatc
     4501 ttcgcccggt agaagcgaat ggcgaaaagc cccaggatgc ccgccaccgc gcccgaggcg
     4561 cccacggccg gctcgggcgt gacgagcggc gtcgcggcca cggctggcag caccttctga
     4621 acgacgacga cgtagagcag ccccccggcc atgcccgatc ccagatagaa cagcatgtac
     4681 tccacccggc ccagcacatc ctcgaccaaa catccaaaga gccagaggta cgtgacgttc
     4741 acaaggaggt ggagtgcgct ggcgtggagg agcatcgagc taaagagcgt cgcgatctcc
     4801 ggggcatcgc cgcgcaatcc gtagttctgg acgatgtaat cgagggtgag cgtgcccccc
     4861 atcagcgaga ggaggaagag gctgcagttc agccccacca aaaagagcgt gacgatgggg
     4921 aaatggcgca gctcgcggtc cgtggcgtat gggatcggga tcagcatcgg cacaccctcg
     4981 gagccctaac cgatctcgcc atctcccgcg ggagagaggc tttccaggat ctcctggacc
     5041 aagtcatccg gctgcacgcc ctccgcgagc ccctcgaagt tgaggagaac gcggtggcgc
     5101 agcgccggca acgcggagcg ccgaatgtca tcgaaagaga cgttgtagcg cccgtctaga
     5161 agcgcacgcg tcttcgcggc gagcacaagc gcctgtgccc cgcgcgggct ggagccgtag
     5221 cgcacgaaac gcttcacctg cggcggcgca agctctgagt ccgggtgcgt cgccatgacg
     5281 atcttcagcg cccactcctt gacgtgggga gcgattggca ccgcccggca gagcgcgcgc
     5341 atctccagga gcgccggtcc gtccaggatc acgcgcagct ccggttcctc cgttccggtc
     5401 gtgcggtcga tgatcgcgtc cagctcctct aggcagggcg ggcccaccag cagcttgaag
     5461 aagaagcggt cgacctgcgc ctccggcaga ggatacgtgc cctcctgctc gattgggttc
     5521 tgggtggcga ggacgaagaa cggttcctcc aggcggcgca cctctcgcgc cacggtcacc
     5581 gattgctcct gcatcgcctc caggagcgcc gactgcgtct tgggcgtggc ccggttgatc
     5641 tcatccgcga ggacgacgtt ggcgaagatc ggccccggct ggaagagaaa ctgccggccg
     5701 ccggagggac tctcggtgac gatgttcgtg ccggtgatat ccgcgggcat caggtccggg
     5761 gtgaactgga ttcgcgagaa cttcaggtgg aggacgcggg ccagcgtgcg caccagaagt
     5821 gtcttcccca aaccgggcac gccctccagg agcacgtgcc cgccagcaaa gacgctggtc
     5881 aacacgccgt cgatgacctc ttcctggccg acgatcacct tgcggatctc gtcgcacacg
     5941 gcggcataac gctcgcggta cgcttcggcg cgggctgcga tctccgtggt gggtgtcata
     6001 ggcacttcgt tcatccctgg gttgatcctg gcgccgcgcc tcacgggcgc agcgagtcga
     6061 agtagtcgcg aacctgcttc cgatagcccg cgggaatctc ctcgcgcgcc agcgcctcct
     6121 ccgcagtgcg ctggtagtga gagtagacct cgtaataggg ccgggaggcg ctgcccggat
     6181 cgggtgcagc gcgcacctcg gaatagtcgc tctttccggc gcgcgttttc ccgcgcacct
     6241 gggtgtcgag ccggttcgtg tcgacgcgat ggggggcgta gacctgctcg aagcgggcgc
     6301 gcatctcgga gcggtgatcc gcctggcgat ctcgcggcgt tcccgattcc atcgtgccgc
     6361 cggccacctg ccgattcgtc accccgcgcc cggcttcaga gccgccgtct tgtgtggcga
     6421 gctggtgagt gccgtggcct tgcgccttct cgccctgggc gccaccagcg tgtgcatcac
     6481 cgcgcgccat cgccccctgg ccgctaggca gaccctcctt gccaccctgc ggggcttcct
     6541 ggcccgccag agccagccgc tggcgcgatt gcatcaccgc ctcc
//
LOCUS       DAOSCV010000102         6580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7487_length_6580_cov_3.732720, whole genome shotgun sequence.
ACCESSION   DAOSCV010000102 DAOSCV010000000
VERSION     DAOSCV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7487_length_6580_cov_3.732720"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1364)
                     /gene="mqnE"
                     /locus_tag="PLY56_03845"
                     /pseudo
     CDS             complement(300..1364)
                     /gene="mqnE"
                     /locus_tag="PLY56_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
     gene            2010..3638
                     /locus_tag="PLY56_03850"
     CDS             2010..3638
                     /locus_tag="PLY56_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ20642.1"
                     /translation="MMKSKSLLLLLLLLCGLLYLTRLGSVTFYEEDEPRFASAVRTMV
                     ETGDYITPRFNGKLRINKPILFYWLAAATSHVVGVGELGARLPSALLATGLVVMAFFF
                     ARAYGSVRFAGLVAFLVATCLQYAVVLGRAATADMTFIFFLTAALFAFFRSETEPSSR
                     RWVLWFWASLALATLAKGPAAVVLAGATVGLFLILRGTLLDGVKRTITIPGLGLFALI
                     AVPWFAVELHLLGKTFWRGFFIAEHFGRYAGTADPHHGPIWFFIPVLLLMFYPWSLFL
                     PAGLAGVLAERRSKEPPLRLYALCWSAVVFGLFSFSGTKLPHYIAPLYPAAAILAARA
                     WEERLAGRASRLWQAGAGVLALLGLLLGGALLFVGLDGATLERLSSGLVTESEPRLVV
                     FGLLLGGAGVLGGALLLARRRPETAFCVMGGLLVAALAWGNIALAPVVARCWQDPVRH
                     LAEDAAARAGEEGQLAIYAMASSGVVYYSKRTVAWVKRHEDTHRLEALARRGPLYVIT
                     KPSRVEKIPARLGLRELRREGAYVLLGPEKGIAP"
     gene            3635..4588
                     /locus_tag="PLY56_03855"
     CDS             3635..4588
                     /locus_tag="PLY56_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ20643.1"
                     /translation="MTAKRGRRSLVLLARGAVGAAVLAYLFSQLQGGEVAAALRSLDA
                     PLWIAAVALYLVGQAVCAWKWGLLAGALGMHRPYRSLLAYYCGGMFANLFLPTTVGGD
                     IGRAAAIAPPDGSLGRGVISVLGDRGSGFIALLLLAGVGVLMEPGLPAWIRLGTLACA
                     ALALAGSIAALAAPDLFRRLGKPLHGAILVCRPPALWIPVAVVSLLFQVLAVGVHVLL
                     GRALGLEIPLGTYVLASTLVAIVAMAPISINGLGTRDAAYVYFLGLAQVPQESGLAFA
                     LSWLALILVSGAIGGLVFSLLAPGQMTFFTRRAPAEGEEGR"
     gene            4590..5237
                     /locus_tag="PLY56_03860"
     CDS             4590..5237
                     /locus_tag="PLY56_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ20644.1"
                     /translation="MVDALIRAGQSLQALDIALFRSINGAWAHPALDLVMLVLTQLGL
                     GSIQVGLLALLYLLGGPAGRHAARVCFIAFLAGGVAAQILKLGVDRVRPALVVDGCHF
                     VGEVLRYHSFPSGHTTTSFALAVVISRYYRRWAALVFVLAAGVGLSRVYVGVHFPGDV
                     LAGAVIGIVTAIICLREVPAVPETHPPHGDDAAGTESAKSSEAVPAPARGRSPAT"
     gene            5484..>6580
                     /locus_tag="PLY56_03865"
     CDS             5484..>6580
                     /locus_tag="PLY56_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ20645.1"
                     /translation="MDGTPWHALTPEEVLQRLESRPEGLDEAEAERRRERHGPNTIRA
                     GRRVSPARIFLAQFTSPLIYILLIAAGISFAAEHGLDGWVILAIVILNALIGFFQEYR
                     AERALEALRALAAPKATVIRGGEPVEIDAEQLVPGDILVIAAGDRVPADARVLEAANL
                     QSEEAALTGESTPTRKHTHAMAPDLSLGERENMLFLGTTVVTGRGRAVVTATGMETEM
                     GAIAREVESGGGMQTPLQRKLAGLGGRLGIAAILLAVLLVIVGLTHDLPLQELFFMGV
                     AAAVSFIPEGLPAVVTVVLAVGVQRMVRRHAIIRKLPAVETLGSATFICTDKTGTLTK
                     NEMTVRAIWLRDEEIRVEGEGYAPEGGFRQEG"
BASE COUNT          878 a         2218 c         2265 g         1219 t
ORIGIN      
        1 acgtaggtca ccgtccggcc cgggttcagg cgcttgcgga cccgagtggc cagcgcgccc
       61 agctcgggca ggttcgggtg gcgcaaaagg gcgacgccgt cctcggcgct caaccgctcg
      121 ccgctttcta cccgcctggc gattgatgaa agcccggtgt ggccgctgct ggaaggctgc
      181 tgcgtcggtc ggctcgcgct caccactctt tcgtggctct ggctctctcg ctcggcgcag
      241 gtggagcgat cctcgcgccg gttttctact gcttcccggc tctcgtaggt tccgccagcg
      301 cgctcgaccg gctcgcgccc cgcctcgcgg atcaagccga ccagctcctc gcaggtgagc
      361 tcgcggcgcg tgccggaacc ggagtcgcgc gtgatctcgt agcggaggac cgtgccttcg
      421 atgtcatccg cgccgtacca gagggcggct tgggccaccg gcacgctcgc catcacccaa
      481 aacgccttga tgtgcgggat gttatcgagc atcagccggc tgacggcgat ggtccgcagg
      541 tcgtcgcacg cggtgggccc gggcacccgc gcgagttgcg agccttccgg gtggaaggcg
      601 aggggcacga aggagaggaa gcctccggtt tcgtcttgaa gcgcgcgcac ccggacgaga
      661 tggttcacgc gctcctcgag gctctcacga tgcccgtaaa ggaggctggc gttggtgtgc
      721 aggccggcct tgtgtaccgc tcgcgaagtc tccagccact cttcggggcg gagcttgcgc
      781 gcgaacagct ccgcgtggag ccggtcgctg aggacttcgg cgcccccgcc cggcatcgag
      841 ccgagcccgg cggccttcag ctctgcgagc acctcctcga gcggcttccc ggcggcctcc
      901 cggatctgct ggagctctac catcgtgaac gccttcacat gcactccggg gcgctcctcg
      961 acgatggcgc ggatcaggtc cagatagtag ctgtagggca gcttcgggtt cacgccgccc
     1021 acgacgtgga tctcggcgat ggggaggtgg agcgcctgcc ggacgcgctc gcggatctcc
     1081 tcgatgctca agacgtaggg cgcgggccca tcctcgcggg cgtggaagga acaaaagcgg
     1141 cacttgcgat cgcagacgtt ggtgtagttg atgtgctggt tgcgcacgaa gtaggcgcga
     1201 ttgccgttta ggcgctcgcg cacaacgttg gccagccagc cgacgaggtt cagcgcgggg
     1261 gtgcggaaaa ggcgcaggcc gtcgtcggcg gaaaggcgct ctcccgcgag tactttctcg
     1321 gcgatctctt ccaggccgct ctcctgaaaa gcttgttcta gcatgagttc caatcgatgg
     1381 gacgcggctc ggggaggcaa taacgacctg cggcggccag cggggcgcgt cttccggacg
     1441 ttccgtccct ctcgccaacc gatggcctcc aggcacctct ggcggcctgc cgttctcccg
     1501 gagcatggga tcatccccca ttataccacg cgaaacgccc gcccgacaaa cgcgagccaa
     1561 gcgaggaccg gctgggattc cgatataagg aatccgaagc ctcggaggta gcgcgcaccc
     1621 cgtcgcgcgt ggcagtaaat ccccgcgctg cttgtgagcg aagccctgcc gagctgggtt
     1681 ggggatactc ccagatgtct caaaacggcg gtggctggga ggggaagccc gaagggcttt
     1741 gctgggaagc agcgcggagc ttcgagctcc gcgcgcgggc ggccctctcc atccgtgttc
     1801 gccctctatc tcggaatccc aggtgcccgc gactcttctt gcgcctctct aaaccgtttg
     1861 ttataatggg acggccgtgt tcctcacccg agcgccggcg ctctagaaag gggtttgaga
     1921 ccggtggcga gaacgacctc caaggcggct gcctgcaccc cgggccggac tcgttccgcc
     1981 cccgtcgctt cccgtcccat cgcatccaga tgatgaagtc aaagtcacta ctgctgctgt
     2041 tactgctcct gtgcgggctc ctctacctca cccggctggg atcggtcacg ttctacgagg
     2101 aggacgagcc gcgcttcgcc tcggccgtgc gcaccatggt ggagacgggc gactatatca
     2161 cgccgcgctt caacgggaag ttgcggatca acaagccgat cctcttctac tggctggccg
     2221 cggcaacctc gcacgtggtc ggcgtgggcg aattgggcgc gcgcctgcca tcggcgctcc
     2281 tcgccacggg gctcgtggtg atggcgttct tcttcgcacg ggcctatggc agcgtgcgct
     2341 tcgccggtct cgtcgccttt ctggtagcca cctgcctgca atatgccgtg gtccttggcc
     2401 gggcggccac ggcggacatg actttcatct tcttcctcac cgcggcactt ttcgctttct
     2461 tccggtctga gacggagcca agctcccggc gctgggtgct gtggttttgg gcctcgctgg
     2521 cgctcgccac tctcgccaag gggcctgcgg cggttgtcct ggcgggcgcg acggtgggcc
     2581 tcttcctgat cttgcgcggc actctcctag acggggtgaa gcgcaccatc acgatcccgg
     2641 gcctcggcct tttcgcgctt atcgcggttc cctggttcgc ggtggagctc cacctgctcg
     2701 gcaagacgtt ctggcgcggc ttcttcatcg cggagcattt cgggcgctat gcgggcacgg
     2761 ccgatccgca ccacggcccg atctggttct tcatccccgt gctcctcttg atgttttacc
     2821 cgtggtcgct cttcctgcca gccggtttgg cgggcgtttt ggctgagcgc cggagcaagg
     2881 agccaccact gcgcctctac gcgctctgct ggtcggcggt tgtctttggc cttttcagct
     2941 tttccgggac gaagctgccc cactacatcg cgccactcta tccggcggcg gcgattctcg
     3001 ccgcccgcgc gtgggaggaa cggcttgccg gacgggcgag ccggctctgg caggccggag
     3061 cgggagtgct cgccctgctc ggcctcctgc ttggaggtgc gctcctcttt gtcggcctcg
     3121 atggtgcgac gctggagcgg ctgagcagtg gtctcgttac cgaaagtgaa cctcgcctgg
     3181 tcgtcttcgg cctgcttctt ggcggcgccg gggtgcttgg cggggcgtta ttgctggcgc
     3241 ggcgccggcc ggagacggcg ttttgcgtca tgggcgggct gctcgtcgcc gcgctcgcgt
     3301 ggggaaacat cgccctggcg cccgtggttg cccggtgctg gcaggatccg gtgcgccacc
     3361 tggcggagga cgcagccgcg cgcgcgggtg aagaggggca gctggcgatc tacgcgatgg
     3421 cttcctcggg cgtggtctac tacagcaagc gcacggtggc gtgggtgaag cggcacgagg
     3481 atactcaccg gctggaggcg ctggcgcggc gcggtccgct ctacgtgatc accaagccca
     3541 gccgcgtgga gaagatcccg gcgcgcctgg gcctgcgcga gctgcgccgg gagggagcct
     3601 acgtcctcct cggacccgag aagggcatcg cgccatgacg gcaaagcgag ggcgccggtc
     3661 gctcgtgctc ctggcgcgtg gcgcggtggg agcggcggtg ctcgcgtacc tcttcagcca
     3721 gctccagggg ggagaggtcg ccgccgcgct gcgctcgctc gatgccccgc tctggatcgc
     3781 ggcggtcgcg ctctacctgg taggccaggc ggtgtgcgcc tggaagtggg gcctcctcgc
     3841 tggcgcgctg gggatgcacc ggccttatcg ttcgctcctc gcctactact gcgggggaat
     3901 gttcgccaac ctcttcctcc caaccaccgt gggcggcgat attggccgcg cggccgcgat
     3961 tgccccgccg gatggctccc tgggccgagg cgtgatctcc gtgctggggg atcgcggctc
     4021 gggcttcatc gccctgcttc tcctggcagg cgtcggcgtg ctgatggagc ccgggctgcc
     4081 cgcctggatc cgcctgggca cgctggcttg cgcggcgctc gctctggcgg ggagcatcgc
     4141 ggcgctcgcg gcccctgatc tcttccgaag actaggcaag ccgttgcacg gggcgatcct
     4201 tgtctgccgg ccgcctgcgc tctggatacc tgtcgcggtg gtatcgctgc tgttccaggt
     4261 gctggcggtg ggggttcacg ttcttctggg ccgggcgctg gggctcgaga ttcccttggg
     4321 cacgtacgtg ctggcgtcca cgttggttgc gattgtggcg atggcgccaa tctccatcaa
     4381 cggcctgggc acgcgcgatg ccgcttacgt ctacttcctg ggcctggcgc aggtcccgca
     4441 ggaatccgga ctggcgttcg cgctcagctg gctggcgctc atccttgtca gtggtgccat
     4501 tggggggctc gtcttctccc tgctggcgcc cgggcagatg acattcttca cgcggcgcgc
     4561 gccggcggag ggcgaggagg gccgttgaga tggtagacgc gctcatccgt gccggacaat
     4621 cgcttcaggc gctcgatatc gctctcttcc ggtcgatcaa cggcgcgtgg gcccacccgg
     4681 cgctcgatct cgtgatgctc gtgctcaccc agcttggcct gggatccatc caggtggggc
     4741 tgctcgcgct cctctacctc ctaggcgggc cggccgggcg ccacgcggcc cgcgtctgct
     4801 ttatcgcctt cctcgccggc ggcgtcgccg cgcagatcct gaagctcggc gtcgaccgcg
     4861 tccgccccgc gctggtggtg gacggctgcc acttcgtcgg cgaggtgttg cgctaccact
     4921 ccttcccctc ggggcatacc accacctcct ttgccctcgc ggtggtgatc agtcgctact
     4981 accgccgctg ggcggcgctt gtcttcgtgc ttgccgccgg cgtcggcctc tcgcgcgtct
     5041 acgtgggcgt gcacttcccg ggcgatgtcc ttgccggcgc cgtgatcggc atcgtcaccg
     5101 ccatcatctg cctgcgcgag gtgcccgccg tgccggagac gcatccccct cacggggacg
     5161 atgccgcggg caccgaaagc gcgaagagtt cagaagcggt tccagctcct gcccgcggga
     5221 gaagccccgc cacctgaaac gaattccggt attagcaggt gcgggcgggc gctttgcccc
     5281 tcgagggcgc gcgaccttgc cagccccgga agccatgccg gaacgcccgg gcctgccggg
     5341 cgcgccccca ggcttctcct gaaatggctc tgccagtgcg ccgcgcgccc ggagcgcgtg
     5401 ggagggctgt ttccgcatgg gcctcgccgg gtaaaaggga gaagcgcctc ccagaggttc
     5461 gcagggaacg cgaagaggag accatggacg gaacaccctg gcatgcgttg accccggaag
     5521 aagtactcca gcgcctggaa tctcgccccg agggcctcga tgaggccgag gcggagcggc
     5581 gacgcgagcg ccacgggccg aatacgatcc gcgcgggccg gcgggtgtcg ccagcgcgca
     5641 tctttctggc gcagttcacc agtccgttga tctacatcct cctgattgca gccggcatct
     5701 ccttcgcagc cgagcatggc ctcgacggct gggtgattct cgccatcgtc atcctgaacg
     5761 ccctcatcgg ctttttccag gagtatcggg cagaacgcgc tctggaggcg ctgcgcgccc
     5821 tggcggcccc gaaggcgacg gtcatccgcg gtggcgagcc ggtcgaaatc gacgcggagc
     5881 agcttgtgcc tggcgatatc ctcgtgatcg cagcgggtga ccgcgtgccg gccgatgccc
     5941 gcgttctgga agccgccaac ctgcaatcgg aggaggccgc gctcaccggg gaatcgaccc
     6001 cgactcgcaa gcacacgcac gcgatggcgc cggatctttc cctcggcgag cgcgagaata
     6061 tgctcttcct cggcaccact gtggtcaccg gccggggtcg cgccgtggtg accgcgacgg
     6121 gcatggagac ggagatgggc gccatcgccc gggaggtgga gagcgggggc ggaatgcaga
     6181 cgcccctaca gcggaagctg gccgggctgg gggggcgcct cggcatcgcc gcgatcctct
     6241 tggcggtgct tctcgtcatc gtgggcctga cccacgacct cccgctgcag gagctcttct
     6301 tcatgggcgt ggctgccgcc gtctccttca tccccgaggg tcttccggcg gtcgtcaccg
     6361 tcgtcctcgc cgtgggcgtc cagcggatgg tgcgccggca cgcgatcatc cggaaactcc
     6421 ccgccgtcga gacgttgggc tctgccacct tcatctgcac cgacaagacg gggacgctga
     6481 ccaagaacga gatgaccgtg cgcgccatct ggctgcgtga cgaggagatc cgcgttgaag
     6541 gagaagggta tgcgcccgag gggggattcc gccaggaagg
//
LOCUS       DAOSCV010000103         6550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7527_length_6550_cov_3.505312, whole genome shotgun sequence.
ACCESSION   DAOSCV010000103 DAOSCV010000000
VERSION     DAOSCV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7527_length_6550_cov_3.505312"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..371)
                     /locus_tag="PLY56_03870"
     CDS             complement(33..371)
                     /locus_tag="PLY56_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20646.1"
                     /translation="MAGSYVEAIARAAQDAQSLVRFLDGLDEHAPATPAAIGHAAQLA
                     DAVERVVYQALQEAYPDWSAKAAADQALESIDAFRAAAQGNDVRLMRAAARGALDHLN
                     RARELEEPAP"
     gene            complement(415..636)
                     /locus_tag="PLY56_03875"
     CDS             complement(415..636)
                     /locus_tag="PLY56_03875"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ20647.1"
                     /translation="MVDVATEAMIHARLAENPRLDAEEIEASVYGPRVVLRGRVRTQR
                     EREEAEAAVRQMRGVEQVDNRLVVTGEPI"
     gene            complement(715..984)
                     /locus_tag="PLY56_03880"
     CDS             complement(715..984)
                     /locus_tag="PLY56_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20648.1"
                     /translation="MKHYMVVVEHTPDECLRTMDELAYHTPELLEQFWFGGIAGEHKG
                     WVIVDADSEQDALEKLPPALRENADVVPVRRYTAHSVRALHRMAA"
     gene            complement(1175..2779)
                     /gene="murJ"
                     /locus_tag="PLY56_03885"
     CDS             complement(1175..2779)
                     /gene="murJ"
                     /locus_tag="PLY56_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ20649.1"
                     /translation="MQPPSTPSSSAERRTIARASLIMFVALTLGRILGFVRDATITHL
                     YGQGNQTDIFNMAFFIPDLIFMLIAGGALTAAFIPVFTDYLTQANEEEAWRVFNILAT
                     ALTLVVTGAVIAAGVFAVPLARRLLDDPRFTPADIEQCAYLMRIVLPAQICFLLGGLM
                     MGAQQSRQRFLVPALGPVIYNLSIIIGGVALSPRLGIAGFSWGALGGAVLGNLLLQYT
                     MVRRMGLRYRPACHLGHPGVRRVIGMMGAVVLSLALPQILTELNRAFGVRISDGVASA
                     LANTNRLMQAPLAMFGQSLAVALLPTLSAYAAARRVADFRETLSRSLRAILFTTIPIS
                     ALMIALSAPLIRMVFEHGEFTASDTALAAPILAAYSIAVPAWSAQAVVARACFALGDN
                     RTPVFAGIGVSILFVLLNLALRGPFGGPGLAAATSFSGLILAVVMGSVLRRHLGGLDG
                     GEIARSAGKTALASLPAAAIAWGIASVVESSLASQAVPPALVGLLQCSLGGGAGLLLF
                     AAGAVALRIPEAHGVWDRIRRKLRRS"
     gene            2926..3204
                     /gene="rpsT"
                     /locus_tag="PLY56_03890"
     CDS             2926..3204
                     /gene="rpsT"
                     /locus_tag="PLY56_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ20650.1"
                     /translation="MPNIKSVKKDVVRSRQRHLRNQSYKSRMKTMVKKARAAIMEHQV
                     DESLIRATESTIDKLAQKGIIHRNAAARRKARLMKLVNKERQAQATQA"
     gene            complement(3261..4421)
                     /gene="holA"
                     /locus_tag="PLY56_03895"
     CDS             complement(3261..4421)
                     /gene="holA"
                     /locus_tag="PLY56_03895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ20651.1"
                     /translation="MDLPYLKTSRQKKVVPDHTVYLVYGREEVLVEDVVAKLVAGARE
                     AAGGDPDVQVLHGGGTTCEQVASELANFSLMGMGKVVILRAAHQMRAPEQARLAALLP
                     RIGPGSLLILTADEANYDSRTRKKRILSDKLETALRDVGVLIEVPTFREQDAERWVVA
                     AAKEAGVTMEPNAAAQMVLLAGADLTRLRNELEKLIAYAGAEKSISVADVNLVVTRSP
                     EATVFELIDAIGDRRPDTALAALKVLLDAGEAEPKILALIARQIRLIWQTKYLAERGY
                     LKRSGVQVPAEVARDLLPKDRSTNVMTQVQRPFLREKLLRQAGAFSWDALHRGLERVL
                     AADLAFKGIEGEVDNPRLVLEMLVLELASGRRPAGGTYREKARPLSREAAGR"
     gene            4602..6140
                     /locus_tag="PLY56_03900"
     CDS             4602..6140
                     /locus_tag="PLY56_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20652.1"
                     /translation="MKAWLLSIVLCLSLVPALAQESPFNLGVEAARQGKHEVAIGHFQ
                     KAVANDPRDSRSLSWLGYLYLKTGKASDAVDCLKKATELNPRAEDALNNLGNAYMETG
                     QVEKAIEAYQAALKIKEDYYDAHYNLGNAYIALGKGSSVENAIKAFKQALSLQPNSAE
                     AHNNLGLAYARMAQSYVAARDRFEAANDKEKAERSLSLGTRLYGQAADEYRAATQADP
                     SNGTYWLNLALAYRELLAINGTTEARQNAIAALKKAVSLRQSDYLAHLALADLYSEGN
                     LLTEAISMYSAAARLKPEEFDPHYRLGILYFKTGAYEPAIAAYTKALQIRPNDPSTLN
                     NLGCVYHHTKKYDEAVDAFAKAAAADTRFVTAHANLATVYTTMGKSEEAVAAWRKVVE
                     LEPRNTKARLALARHALNANDLERAEALYREVIGIDARSTEAHNALGFILWRSGKLDE
                     AIQAFNKALTINPKYAPALNNLGAMYEEKGQTDKARDMYKRALAVDPSYEDARKNLDR
                     LGVQ"
     gene            6137..>6550
                     /locus_tag="PLY56_03905"
     CDS             6137..>6550
                     /locus_tag="PLY56_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ20653.1"
                     /translation="MSGSDDTSGGEPVRSLGIVVNEEKPAAVSLGEELHLWLTDQGVP
                     SQVIHLRQEVGADQQWRAHEGGPPPKRLFGDALVVLGGDGTLLATARIAAPAGQPMLS
                     VQLGGFGFLAECQPEQTRECVERILAGGYRIAERMM"
BASE COUNT         1171 a         2178 c         2162 g         1039 t
ORIGIN      
        1 atttcaccct cctgacttgc ggcacgggct gatcatggcg cgggctcttc tagctcccgg
       61 gcgcggttca ggtggtcgag cgccccgcgg gcagcagcgc gcatcaggcg cacatcgttg
      121 ccctgcgcgg cggcgcggaa cgcgtcgatg ctctccagcg cctggtctgc cgcggctttg
      181 gccgaccagt cgggatatgc ctcctggagg gcctggtaca cgacgcgctc cacggcatcg
      241 gccaattggg ccgcgtggcc gattgccgcg ggggttgctg gcgcgtgctc atccaggcca
      301 tccaggaagc gcacgaggct ctgggcatcc tgcgccgccc gtgcgatcgc ctccacatag
      361 ctgcccgcca tggccgctcc ctcctgccca cctcgctccc gagcgggcct tgcctcagat
      421 cggctcgccc gtcaccacca gtcgattgtc cacctgctcg acgccacgca tctgccggac
      481 cgcagcctcc gcctcttccc gctcgcgctg ggtgcgcacc cgtccgcgca gcacaacccg
      541 gggtccatac acggacgcct cgatctcttc cgcgtcgagg cgtgggttct cggctagacg
      601 cgcgtggatc atcgcctccg ttgccacatc caccatgagc ttccctcctg ggacaatctg
      661 ggtgctggat gccggcggcg ttccttgctc gtggtgccgg agacaccgtg gcctttacgc
      721 cgccatccgg tgcaatgccc tcaccgagtg cgccgtgtag cggcggacag ggacgacgtc
      781 ggcattctcg cgcagcgcgg gcggaagttt ctcgagcgcg tcctgttcgc tatcggcgtc
      841 cacgatcacc catcctttgt gctcgcctgc gatccccccg aaccagaact gctccagcaa
      901 ttccggggtg tggtaggcca gctcgtccat ggttctcaag cactcgtctg gagtgtgttc
      961 cacgaccacc atgtagtgtt tcatcgttga accgctcctt tccggcgctg gcaatggccg
     1021 gggcgcgcca ctcgtatagg gatttgttcc ccattcggac aggtgtaaaa cggcgggacg
     1081 cccggctgga ggcaggggaa agcggatggc ccgcaggccg ggggagcgtc tggagaaaag
     1141 gcctgcctgt gccgggcgcc ttgacggcta tgcttcagga gcggcgcagc ttgcggcgaa
     1201 tccggtccca gacgccgtgc gcttccggaa tgcgcagcgc cacggcaccg gcggcgaaaa
     1261 ggagcaggcc ggctccaccg cccaggctgc actggagcaa ccccaccagg gccggcggaa
     1321 cggcctggct ggcgagagag ctttctacaa cgcttgcgat gccccacgct atcgcggcgg
     1381 ccgggagcga ggcgagtgcc gtcttgcccg cagatcgagc aatctcgccg ccatccaggc
     1441 cccccaggtg gcggcggaga acgctcccca tcaccacggc gaggatgagg ccggagaacg
     1501 aggtggccgc cgcgagaccc ggcccgccga aaggtccgcg cagcgccagg ttgaggagga
     1561 cgaagaggat gctcacgccg atcccggcga agacaggcgt gcggttatcg cccagggcaa
     1621 agcaggcgcg cgccaccact gcctgcgccg accaggccgg cacggcgatg ctgtaagcgg
     1681 ccaggatggg ggcggccagg gcggtatcgc tggcggtgaa ctcgccatgc tcgaagacca
     1741 tgcgaatgag cggcgcgctc agcgcgatca tcagcgccga gatcggaatt gtggtgaaga
     1801 gaatggcgcg cagcgaccgg ctgagcgttt cgcggaaatc ggccaccctg cgcgcggccg
     1861 cgtaggcgga gagggtgggc agcaacgcca cggcgagcga ttgcccgaac atggcgagcg
     1921 gcgcctgcat cagccggttc gtgttggcga gcgcggacgc gacgccatcg gagatccgca
     1981 ctccgaaggc gcggttcaac tccgtcagaa tctgcgggag ggcgaggctg agcaccactg
     2041 cgcccatcat tccgatcacg cggcgcactc ccggatggcc cagatggcac gccggccgat
     2101 agcgcaggcc catccgccgg accatggtgt actgcagcag cagattgccc agcactgcac
     2161 cccccagcgc tccccacgaa aagccggcga taccgagccg aggcgatagg gcaacgcccc
     2221 cgatgatgat cgacaggttg tagatcaccg gccccaacgc cggcaccagg aaacgctggc
     2281 gagactgctg ggcgcccatc atcaggcccc cgaggagaaa gcagatctgt gccgggagca
     2341 cgatgcgcat caagtaggca cactgctcga tgtccgcggg ggtgaagcgc gggtcgtcga
     2401 gaagccggcg cgccagcggc acggcaaaga cgcccgccgc gatcaccgcg ccggtgacca
     2461 ccagcgtgag cgccgtggcg aggatgttga agacgcgcca cgcctcctcc tcgttggctt
     2521 gcgtcaggta gtcggtgaag accgggatga acgccgcggt gagcgcgccc ccggcgatca
     2581 gcatgaagat caggtcgggg atgaagaacg ccatgttgaa gatgtcggtc tggttcccct
     2641 ggccgtagag atgggtgatc gtggcatctc ggacaaaccc gaggatgcgc ccgagggtga
     2701 gcgccacgaa catgatgagc gaggcccggg cgatggtgcg gcgctcggcg ctgctggaag
     2761 gggtggaggg tggttgcatt cttggcctgg tcatgctata atcacgaggt acgcccccgg
     2821 gcggttctct gcccggcgtg ttggcatgca attttgagcg accgcggtga cgccgcgctc
     2881 tccgcggacg gagaccgatg cagatcgcct ggaggactcg aacgaatgcc gaatatcaaa
     2941 tccgttaaga aggatgttgt gcgctcgcgc cagcgccacc tgcgcaacca gtcctacaag
     3001 tcgcgcatga agacgatggt gaagaaggcc cgcgcggcga tcatggaaca tcaggtggat
     3061 gagtcgctga ttcgggcgac cgagagcacc attgacaagc tcgcccagaa ggggattatc
     3121 caccggaacg ccgcagcgcg ccgcaaggct cgcttgatga agctggtgaa caaagagcgg
     3181 caggcccagg ctacccaggc ctaacttcat gcggccggtc tggccgtgag aaacgcgcgc
     3241 ccgcagcggc atcagggctg tcatcggcct gccgcttccc gagagagggg gcgcgctttt
     3301 tctctgtacg tcccaccggc gggccggcgc ccgctcgcca gttccagcac caacatctcc
     3361 agcacgaggc gcgggttgtc cacctcgccc tcgatccctt tgaaagcgag atccgcggcc
     3421 agcacgcgct ccaggccccg gtgcagcgcg tcccaggaga acgcgccagc ctggcgcagg
     3481 agcttctcgc gcaagaaggg ccgctgcacc tgggtcatca cattcgtgga gcgatccttg
     3541 ggcagcaggt cgcgcgccac ctcggccggc acctgcacgc cgctacgctt caggtagcca
     3601 cgctcggcca ggtacttcgt ctgccagatc agacggatct gccgggcgat cagcgccagg
     3661 attttcggct cggcctcgcc ggcatcgagc aacaccttga gcgccgccag ggctgtatct
     3721 ggccgccggt cgccaatggc gtcgatgagc tcgaagaccg tggcctctgg tgaacgggtg
     3781 accacgaggt tcacgtcagc tacgctgatc gatttttcgg cgcccgcgta ggcgatcagc
     3841 ttctccagtt cgttgcgcag ccgggtgagg tcggcgccgg cgaggagtac catctgcgcc
     3901 gcggcgttcg gctccatcgt gaccccggcc tccttggccg ctgccaccac ccagcgctcg
     3961 gcatcctgct cgcggaaggt gggcacctcg atgaggaccc ccacatcgcg gagggccgtc
     4021 tccagtttgt cgctcaggat ccgcttcttg cgcgtccgcg agtcgtagtt ggcttcgtcg
     4081 gcggtcagga tcagcaacga gccggggcca attcgcggga ggagggcagc gaggcgcgcc
     4141 tgctcggggg cacgcatctg atgggcggcg cgcaggatca ccaccttgcc catgcccatc
     4201 agcgagaagt tggccagctc gctggccact tgctcgcacg tcgtgccgcc gccgtgcagc
     4261 acctgcacat cgggatcgcc tccggctgcc tcgcgcgcgc ccgccaccag cttggcgacg
     4321 acatcttcga ccagcacctc ctcacgcccg taaacaaggt agacggtgtg gtcgggcacc
     4381 accttcttct ggcgggatgt cttcaagtac gggagatcca cggctcttcc tcactctgtg
     4441 tgtccggtcg cggcggtatt ataccaccgc gggacggcgc aagtccagcg ccaggggccc
     4501 cgagcgaggc acgatgcgcc ttgccatcac acaagcgttc tgatataatg gctccagttg
     4561 gcctatcttg ttaccctttc agattgcgag gtgtggttgt gatgaaggcg tggctgctct
     4621 ctatcgtgtt gtgcctatcc ctggtccccg cactcgccca ggagagccct ttcaaccttg
     4681 gggtggaagc ggcacgccag ggcaagcatg aagttgccat cggccacttc cagaaggcag
     4741 tggccaacga cccgcgtgac tcgcgcagtt tgagctggtt ggggtacctc tacctgaaga
     4801 ccggcaaggc cagcgacgcc gtcgattgcc tgaaaaaggc gaccgagctg aaccctcgcg
     4861 ccgaggacgc cttgaacaac ctgggcaatg cctacatgga gaccggccag gtggagaagg
     4921 cgattgaggc ctaccaggcg gcgctgaaaa tcaaagagga ctactacgac gcccactaca
     4981 acctgggcaa tgcctatatc gcgctcggca agggctcgtc cgtagagaat gccatcaagg
     5041 cgttcaagca agccctgagc cttcagccca acagcgccga ggcgcacaac aacctcgggc
     5101 tggcctacgc ccgcatggcg cagagttatg tggcggcgcg cgaccgcttc gaggccgcca
     5161 acgacaagga gaaggcagag cgctcgctca gcctcggaac gcgcctctac ggccaggccg
     5221 ccgacgagta tcgcgcggca acgcaggccg atccctccaa cggcacctac tggctaaacc
     5281 tggctctcgc ctatcgcgag cttctggcga tcaacggtac gactgaggcg cgtcagaatg
     5341 ccatcgccgc gctgaagaag gcggtctcgc tgcggcagtc ggattacctg gcccacctgg
     5401 cgctcgccga tctctactcc gagggcaacc tcctcaccga ggcgatctcg atgtactccg
     5461 cagcggcgcg cctgaagccg gaggagttcg acccgcacta tcgcctggga attctctact
     5521 tcaagacggg ggcttacgag ccggcgattg ccgcctatac caaggcgctc cagatccggc
     5581 ccaacgatcc cagcaccctc aacaacctgg gctgcgtgta ccaccacacc aagaagtatg
     5641 atgaggcagt ggatgccttc gcgaaggccg cggcggcaga cacgcgcttc gtgacggcac
     5701 acgccaatct ggcgacggtc tacaccacga tggggaagag tgaagaggcg gtcgctgcgt
     5761 ggcgcaaggt ggtggagctg gaaccgcgaa acacgaaggc gcgcctggcg ctggccaggc
     5821 acgccctcaa cgccaacgat ctggagcgcg ccgaagccct ctaccgcgag gtgatcggta
     5881 tcgacgcgcg cagcacggaa gcccacaacg ccctcgggtt catcctgtgg cgctcgggca
     5941 agctagacga ggcgatccag gcattcaaca aggcgctcac catcaacccg aagtacgcgc
     6001 ccgccctcaa caacctgggt gcgatgtacg aggagaaggg ccagacagat aaggcgcggg
     6061 atatgtacaa gcgcgccctg gccgtcgatc cctcctacga ggatgcccgg aagaacctgg
     6121 accgcctggg ggtgcaatga gcggcagcga cgacacttcc ggcggtgagc cggttcgctc
     6181 gctcgggatc gtggtcaacg aggagaagcc ggcagcggtc tccctgggag aggagctcca
     6241 tctctggctg acggatcagg gagtcccctc gcaggtgatc caccttcgcc aggaggtggg
     6301 ggcggaccag cagtggcgtg cgcacgaggg cggtccacct cccaagcggc tgtttggcga
     6361 cgcgcttgtg gtccttgggg gcgatggcac cctcctcgcc acggcgcgta ttgccgcgcc
     6421 cgcgggccag ccgatgctca gcgtgcagct aggtggcttc ggcttcctcg ccgagtgcca
     6481 gccggagcag acccgcgagt gtgtcgagcg gatactcgcc ggcggctacc gcattgccga
     6541 gcggatgatg
//
LOCUS       DAOSCV010000104         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7535_length_6546_cov_3.629795, whole genome shotgun sequence.
ACCESSION   DAOSCV010000104 DAOSCV010000000
VERSION     DAOSCV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7535_length_6546_cov_3.629795"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLY56_03910"
     CDS             <1..237
                     /locus_tag="PLY56_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20654.1"
                     /translation="AREGVEDYEYLNLLRQLVAKAGPRAKGLDAAKKALEKARALVSI
                     PNAGGRYSTKILPDPDALLAAREAVAKAIEGLAR"
     gene            242..958
                     /locus_tag="PLY56_03915"
     CDS             242..958
                     /locus_tag="PLY56_03915"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20655.1"
                     /translation="MAVLDNWFIQVHDFIYVKEEEGSDAEAECILRLLGLRAGDRVLD
                     APSAAGRIAVRLARAGCHVTAVDINHDYLKRARARFQAEGLKGEFCQADLRRLPLREG
                     FDAVCNWWSSFGYFSDAENLRVLRGMVAALRPGGRLIIEQPNRHFVLRRLRPVMTESD
                     WVLLTNWNARTQRLESVWIHEEDGREERYPMSIRWYTWAQFRSLFRKAGLTLEAAYGD
                     HDGSDYTPSSRRLIVVGRKG"
     gene            1092..4043
                     /locus_tag="PLY56_03920"
     CDS             1092..4043
                     /locus_tag="PLY56_03920"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ20656.1"
                     /translation="MTFSHSLAMFLLLVALAAPAISAPAAPVTLQSGDWTATLGPTGL
                     ALTHRGEVISGGSYFHVFAPEYQGAVFSSTDAWNKGTVEVSPDGRTATLTADLQNGPF
                     SYRLELDGNTARITIRLSVKDGVPVGPVECSLLQFPEPLTKDGVIEVWNAAGLVTHRE
                     PIPATPVRGGLAPSGQGFTLKTPARKIVIEALDFGTVYPFDARHERYASRRGVWSFSS
                     PPARAGGETVARFVVRVEPPDPARSPGRITLAPRADATAILTGTNPTARETLATDELA
                     SILEEMIGRRLERGVASGKGAPAGTILVGQAAVAAGLISQKELNAVAPDGYVVRVRGG
                     RAGICGWRDVGTVYGAYALLRHLGCRFYAPNCETIPRRDTLVIPECTLSAKPFFEFRN
                     LTGNLKLGHTPKDDIMDPRAIGEPGNIVHSAEYLLPYDTYHKEHPEYFALQKDGRRLT
                     RDPDARRFDVHLCLANPDVRRISAERLLALIEKQPDRTFFGVSQGDGHAWCECEECKR
                     LDGAPGVMTDRLLDYVNHIAREVARKYPDKRILTLAYTNATSPPPTRVFPEPNVMVQY
                     CPYPPRTGCSSHDLTCEKNTQSQQDLLGWLEKCPKNMYIFDYPTGYQNIYEPFGSFYA
                     MKRKLELYATRGVRGIFYCGTPRNFQDLFIYVQSHLLWNPDTPVEPLIDEFMRAYYGP
                     AAPKVRAYFDFMHREVDERPIHQMCEGPSTHFVTPEYAETALKLLGEAEEAARDDRAA
                     LYRVRVEKLCVLFGDLNARNPFNGRLVVSEDAFARRLAEFARIARTMRLGGFVRRLPP
                     EEWLQRVARIKPERKPWYLDPVIERLATDPAGTLAAEQQRYLQTEIPGGWRLELEGFR
                     GARGPEEYGYLCPPRRAIWIYGTNTEHPRMWTVLHLKAAPKGAAQLVLTAQDDDKPGA
                     VPVRVSLNGRVLFEGPNPFRERGWSTESLAIPAGVLRQGENELRIETLGESRARDAGW
                     FMLADCQVLVP"
     gene            4294..5196
                     /locus_tag="PLY56_03925"
     CDS             4294..5196
                     /locus_tag="PLY56_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ20657.1"
                     /translation="MSERIPVLLDTDIGSDIDDAVCLSYLLRQPRCELVGVTTATGNV
                     VQRAALAAEICRAAGRDDVPIHAGAPGPLLFGPGQPNVPQYEAVASRPHRTDFPPGAA
                     LEFMRQTIRQRPGEIVLLAIGPMTNLGLLFAADPEIPSLLKELVLMCGVFTAGNGHGP
                     GAREWNAFCDPVATAITYRARPPRFTSIGLEVTTRCILGADECRARFQKAGGPLSIVA
                     EMAEVWFRGSPRITFHDPLAASVIFEEGLCRYQEGLVEVETTSQRLGGLTTWNPNSEE
                     KPHRIAVSVDSEAFFAHYFGVVGG"
     gene            5345..>6546
                     /locus_tag="PLY56_03930"
     CDS             5345..>6546
                     /locus_tag="PLY56_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20658.1"
                     /translation="MMIRILMALLLTTTAVIAAEEVVGKRPYEMEWANRTEDHQPPLV
                     DFEDISGWKVEGHNSVASLVRTREQQLWGKYVAKLTYRATASGGPEVRIMPPQPIPIT
                     RPFDAVTLWVYGNTWGYARDVTTPSVGITALFEDAQGEEFGVYLYHVDWKEWNLLHKR
                     LTPEQIARAEKGARFKGIAITGGRNKEDRVLYFDNLAVYKEEFAPLKFEPRPQRGIPM
                     FPGQSVGTNTDPNKKLPFPNRLETILPDNLAKEFKTYFRAESAAYVFSYVGSDGKLTY
                     RLEPKTGTWSDITAQWTPANGKPGPVIRPCMGGGVYLQTPDGPKAPEKIELLSARVEN
                     NVPTLRYRVAVGGVSAEVTYAYRLWNKSLVIDTICLGGQVGEVRYGRALGLENPRLVT
                     NPYYRAEGG"
BASE COUNT         1100 a         2165 c         2186 g         1095 t
ORIGIN      
        1 gcccgagaag gcgtggaaga ttacgagtat ctcaatctgc tgcgccagct cgtggcaaaa
       61 gccggcccgc gcgcgaaggg actggatgcc gcgaagaagg ccctggagaa ggcacgcgcg
      121 ctcgtctcga tcccgaacgc cggcgggcgc tactccacga agatccttcc ggacccggac
      181 gccctcctcg ccgcgcgcga ggccgtggcc aaagcgatcg aagggctggc gaggtagtac
      241 gatggcggtg ctcgacaact ggttcatcca ggtgcatgac ttcatctacg tgaaggaaga
      301 agaggggagc gacgcggagg cagagtgcat tctccgcttg ctggggctgc gcgcgggcga
      361 ccgggtcctc gatgccccga gcgcggcggg acgcatcgcc gtgcgtctgg cgcgcgccgg
      421 gtgccatgtg accgcggtgg atatcaatca tgactatctg aagcgtgccc gcgcccgctt
      481 ccaggcagag ggcctgaaag gtgaattctg ccaggcggat ctgcgccgcc tcccgctccg
      541 ggaaggcttc gacgcggtct gtaactggtg gtccagcttc ggctacttct ccgacgcgga
      601 gaacctgcgg gtgctgcgcg ggatggtggc cgcgctccgc ccgggtggcc gcctcatcat
      661 cgagcagccg aaccgacact ttgtcctccg ccgcctccgt ccggtgatga ccgagagcga
      721 ttgggtccta ctcaccaact ggaacgcccg cactcagcgc ctggaatcgg tgtggatcca
      781 cgaggaggat ggccgcgagg agcgctaccc gatgtcgatt cgctggtaca cctgggcgca
      841 gtttcgctcg ctcttccgga aggccggcct taccctcgag gcggcctatg gcgaccatga
      901 tgggagcgac tacaccccct cctcgcgccg gctcatcgtc gtcggccgga aagggtgagc
      961 gcgccagggc cccggatgcc gccggacaca ggaaacgccg cgggaaacgc gaaggaacag
     1021 agcaacgcga agccctcacg tggcctggtt gcccgcgcct cgcgagagct tctcgtcggg
     1081 aggtgacggc catgaccttc tcgcactcgc tcgccatgtt tctgctgctt gtcgctctcg
     1141 cggcgcctgc gatttcggcg ccagccgccc cggtgacgct tcagtctggt gattggaccg
     1201 cgacgctggg acccacgggc ctcgccctca cgcatcgcgg cgaggtgatc tcgggtggct
     1261 cctactttca cgtcttcgcc ccggagtatc agggagcggt cttcagctcc actgatgcgt
     1321 ggaacaaggg gacggtggag gtctctcccg atggccggac ggcgaccctc accgccgatc
     1381 ttcagaacgg ccccttctcc tatcggttgg agctggatgg gaacaccgca cggatcacga
     1441 tccggctctc ggtgaaggat ggcgtcccgg tagggccggt ggagtgctcg ctcttgcagt
     1501 tcccggaacc cctcaccaag gacggcgtga tcgaggtgtg gaatgccgcg ggcctggtca
     1561 cgcatcgtga gcccataccg gcgacgccgg tgcgtggcgg cctggcgccg tcgggccagg
     1621 ggttcacgtt aaagacgccg gcacgcaaga tcgtcatcga ggcgctcgac tttggcaccg
     1681 tctacccctt tgatgcccgc catgagcgct atgcctcgcg gcgcggcgtg tggtctttca
     1741 gctcgccccc ggcgcgcgcg gggggcgaga ccgtcgcgag gttcgtcgtc cgtgttgagc
     1801 cgcccgatcc cgcgcgctcc ccgggccgga tcactctcgc cccgcgagcg gacgcgaccg
     1861 cgatccttac cgggaccaac ccgaccgcgc gcgagacact ggcgaccgac gagctggcga
     1921 gcattctgga ggagatgatc ggccggcgcc tggagcgcgg ggtggcgagc ggcaagggag
     1981 cccccgccgg gacgatcctg gtgggacagg ccgcggtggc cgcggggctc atctcgcaaa
     2041 aggagcttaa cgccgtggcg cccgatggct atgtcgtgcg cgtgcgcggc gggcgcgcgg
     2101 ggatctgcgg gtggcgcgat gtcgggacgg tttatggcgc gtatgcgctg ctccgccacc
     2161 tgggctgtcg cttctacgcg cctaactgcg agacgatccc ccggcgagac acgcttgtga
     2221 tccccgagtg caccctttcc gcgaagccgt tcttcgagtt tcgcaacctc accggcaacc
     2281 tgaagctggg gcataccccg aaggacgata tcatggaccc gcgcgcgatt ggcgagccgg
     2341 ggaatatcgt ccattcggcg gagtacctgc tcccctacga cacctaccac aaggagcacc
     2401 ccgaatactt cgcgcttcag aaggatggcc ggcgcctcac ccgggatccc gacgcgcgcc
     2461 ggtttgacgt ccacctttgc cttgccaatc ccgacgtgcg ccggatctcc gccgagcgcc
     2521 tgctggcgct catcgagaag cagccggacc gcaccttctt cggcgtctct cagggcgacg
     2581 gccacgcctg gtgcgagtgc gaggagtgca agcgcctgga tggcgccccc ggagtgatga
     2641 ccgaccgcct cctcgattac gtgaaccaca tcgcgcgcga ggtggcgcgc aagtacccgg
     2701 ataagcgcat cctcacgctc gcctacacga acgccacttc acccccgccc acgcgcgtgt
     2761 tccccgagcc gaatgtgatg gtccagtact gcccctatcc cccgcgcacc gggtgcagca
     2821 gccacgacct cacctgcgag aagaacacgc agagccagca ggacctcctc gggtggctcg
     2881 aaaagtgccc caagaacatg tacatcttcg actacccgac cggctaccag aacatctacg
     2941 agccgttcgg cagcttctac gcgatgaagc ggaagctgga gctctatgcc acgcgcgggg
     3001 tgcgcggcat cttctactgc ggcacgccgc gcaacttcca ggacctcttc atctacgtcc
     3061 agagccacct cctgtggaat ccagataccc ccgtggagcc gctgattgat gaattcatga
     3121 gggcctatta cggcccggcg gcgccgaagg tgcgcgccta cttcgatttc atgcaccgcg
     3181 aggtggacga gcgccccatc catcagatgt gcgagggccc cagcacgcac ttcgtcaccc
     3241 cggagtatgc cgagacggcg ctgaagctcc tgggcgaggc agaggaggcc gcgcgggacg
     3301 accgcgccgc tctttaccgg gtgcgcgtcg aaaagctgtg cgtgctgttt ggtgacctga
     3361 acgcccggaa cccgttcaat gggcggctgg tggtaagcga ggatgccttt gcccggcgcc
     3421 tggcggagtt cgcgcggatc gcccgtacca tgcgccttgg cgggtttgtc cgccgcctgc
     3481 ccccggagga atggctgcag cgcgtggccc ggatcaagcc ggaacgaaaa ccgtggtacc
     3541 tggacccggt gatcgagcgt ctggccaccg atccggcggg cacactggcg gcagagcaac
     3601 agcgctacct gcagacggag atccccggcg gctggcggtt ggagctcgag gggttccgtg
     3661 gcgcgcgcgg ccccgaggag tatggctacc tgtgtccccc gcggagggcg atctggatct
     3721 acggcaccaa caccgagcac ccgcggatgt ggacggtgct tcacctcaag gctgcgccga
     3781 agggcgcggc ccagctggtg ctcaccgccc aggatgatga caagcccgga gccgttcccg
     3841 tgcgcgtttc gctcaacggg cgcgtgcttt tcgaggggcc gaacccgttc cgcgagcgcg
     3901 gctggtccac cgaaagcttg gccatccccg cgggcgtgct gaggcagggc gagaatgaac
     3961 tccgcataga gaccctcggg gaatcccgcg cgcgcgatgc cggctggttc atgctcgccg
     4021 attgccaggt gctcgtgccg tagccagggc gaagcgcccc ggaggagcgt cggcgcgaat
     4081 cgtctccagg agatggagtg ctcgtcgctt ctccggggct gttccacgcc agacccccta
     4141 cgcccgagat ccgagtggca acctccctgg catgaatccg ccaggcgctt gtcgaacact
     4201 gaagcacgcc tggcgtgcag gcatcccagg gttcgttatc gaaccgggtt tgcgaagcgc
     4261 actctttcat aggcctgagc cacgggaggt cccatgagcg aacgtatacc ggttctcctc
     4321 gataccgata ttggcagcga catcgatgat gccgtttgcc tttcctacct gctccgccag
     4381 ccgcgctgcg agctggttgg cgtgacgacg gcgacgggga atgtggtcca gcgcgcggcg
     4441 ctagccgcgg agatctgccg tgccgccggg cgcgacgacg tgccgattca tgccggcgcc
     4501 cccggaccgc tcctcttcgg gccgggccag cccaacgtgc cgcagtacga ggcggtcgcc
     4561 tcgcgcccgc accgcaccga cttcccgcca ggcgcggcgc tcgagttcat gcgccagacg
     4621 atccggcaac gcccgggcga gatcgtgctt ctggcgatcg gcccgatgac gaacctcggc
     4681 ctgcttttcg cggcggatcc ggagatccca tcgctcctta aggagctggt gctgatgtgc
     4741 ggcgtcttca ccgcggggaa cgggcacggc cccggcgcgc gcgagtggaa cgccttttgc
     4801 gacccggtag ccacggcgat cacctaccgc gctcgcccgc cgcgcttcac ctccattggc
     4861 ctggaagtga caacccgctg catacttggc gctgatgaat gccgcgcccg cttccagaag
     4921 gccggcggcc cgctcagcat cgtcgcggag atggcggagg tctggttccg cggcagcccg
     4981 cggatcacat tccacgaccc acttgctgcc agcgtgatct tcgaggaagg gctgtgccgc
     5041 tatcaggagg gcctggttga agtggagacg accagccagc gactgggcgg cctgacgaca
     5101 tggaatccca actccgagga gaaaccgcac cgcatcgcgg tcagcgtcga ctccgaggcg
     5161 ttctttgcgc actacttcgg ggttgtgggg ggatagacgc acgcatcgct tcgtgagaag
     5221 gggcatcctg gagttggggc cggcgcggga tgctccagag accaggtatc cacgcgcaga
     5281 gcaaggcgtg gccgggcgcc cagcgtcgct tctcccttgc ggtgtatgtc tggaggtagc
     5341 tgtagtgatg atacgcattc tgatggcgct gcttctgacg accacggcgg tcatcgccgc
     5401 cgaggaggtg gtcggcaagc gcccctatga gatggagtgg gccaatcgaa cagaggacca
     5461 tcagccaccc ctggtcgatt ttgaggacat ttcggggtgg aaggtggaag gccacaattc
     5521 cgtggcaagc ctggtccgca cccgtgagca gcagctgtgg ggcaagtacg tcgccaagct
     5581 cacctaccgg gccacggcca gcggcggccc agaggtgcgg atcatgcctc cacagccgat
     5641 cccgatcacg cgcccgtttg atgccgtcac cctctgggtt tatggcaaca cctggggcta
     5701 cgcgcgtgac gtcacgaccc cttccgttgg gattacggcg ctcttcgagg atgcccaggg
     5761 ggaggagttc ggtgtctacc tctatcatgt ggactggaag gagtggaatc tcctccataa
     5821 gcgcctgact ccggagcaga tcgcgcgcgc tgagaagggc gcccgcttca agggaatcgc
     5881 gatcaccggc ggcaggaaca aggaagaccg cgtcctctac ttcgacaacc tggctgttta
     5941 caaagaagag ttcgcgccgt tgaagttcga gccgcggccc cagcgcggca tcccgatgtt
     6001 ccccgggcag tcggtgggca ccaacaccga tcccaacaag aagctcccct tcccgaatcg
     6061 cctggagacg atcctgccgg acaacctggc caaggagttc aagacttact tcagagcgga
     6121 gagcgcggcc tacgtcttct cttacgtggg ctctgatggg aagctgacct accgcctgga
     6181 gccaaagacg ggcacgtgga gcgatatcac ggcgcagtgg acgcccgcga atgggaagcc
     6241 gggccccgtg atccggccgt gcatgggcgg tggggtctat ctgcagacgc cggatggccc
     6301 gaaggccccg gagaagatcg agcttctgtc ggcgcgtgtc gaaaacaacg tccccacgct
     6361 tcgctaccgc gtcgccgtgg gtggggtttc ggcggaggtg acctacgcct atcggctttg
     6421 gaacaagtcg ctggtgatcg ataccatctg cctgggcggg caggtgggcg aggtgcgcta
     6481 cgggcgtgcc cttggcctgg agaacccgcg gctcgtgacg aatccgtact accgcgccga
     6541 gggcgg
//
LOCUS       DAOSCV010000105         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7567_length_6515_cov_3.091176, whole genome shotgun sequence.
ACCESSION   DAOSCV010000105 DAOSCV010000000
VERSION     DAOSCV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7567_length_6515_cov_3.091176"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLY56_03935"
     CDS             complement(<1..220)
                     /locus_tag="PLY56_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOJ20659.1"
                     /translation="MEVGIYGLGRMGANMARRLVNGGHRVVVFNRSRGPVEAAAAFGA
                     VPSDSLADFAGKLSAPRIVWLMLPAGDTT"
     gene            481..1284
                     /locus_tag="PLY56_03940"
     CDS             481..1284
                     /locus_tag="PLY56_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20660.1"
                     /translation="MVVDIHTHAFPNDLAPRAVKKLSEVARIPARTDGTCEGLRTSMR
                     RAGVDLSVIMPIATKPTQVRTINAWAAEVNATYEDLLSFGTLHPLQEDWAEEIERLVA
                     DGIRGVKLHPDYQEFFVDDAAMIPIYRALAKARRILLLHSGVDIGIPPPVHCTPDRLA
                     RVLDAVPELTVIAAHMGGYLRWEAVEQFLIGRDLYLDASYSLADLGPDALVRLARAHG
                     IERVLFGTDSPWTDQATELAAFRDLPLSEEEKAALLGGNARRLLGLSAG"
     gene            complement(1576..2868)
                     /locus_tag="PLY56_03945"
     CDS             complement(1576..2868)
                     /locus_tag="PLY56_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOJ20661.1"
                     /translation="MNRIRPLVAVVVLLGAVLWHGMPASADILPLDAEISIGREAAQI
                     IEEQYPISTDAILAARVQRIGRRIAGVCGRPDLPFSFKVIDTPDINAFCLPGGFVYVF
                     RGLLQGVPDDDALAFILAHEIAHAVHRHALRQFEKSQILRVVTAPLGSLLGALGRDLT
                     HVILSRRFSRADELQADREGLVYTVRAGFDRQGGLKAMETLLQLAERSRHPEFLMSHP
                     NTHRRKRVLEALAAELDAAPPDRPQITALPIPDPVWSMPPPSVPTPRFPLAIGDEWTY
                     RLRARDGSEVNAVTRVVEVHPARPEGIFRVVTEYPGGFSTQAWVTTTASGILTHPAGE
                     GSEGPWQAEWSWESLGETRPLEKITVPAGDQECVRVIQTDAKGALIAEGWFAEGVGLV
                     KAVWHHQGLTQELVSYRAALRANRAGQARVGTSDPADS"
     gene            complement(2865..5114)
                     /locus_tag="PLY56_03950"
     CDS             complement(2865..5114)
                     /locus_tag="PLY56_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20662.1"
                     /translation="MSYRRSIAVCLCVALLAAPGGALAASETRLAIARDWGRVTGYVL
                     DGATGEPVAGARVVVEEGGAFADRGPTTGQTNAEGRYQVRARIGRKSSHLDVMSLVEG
                     FPIGLLMPWGLFKQTRVVDARQCLMQVDAPGYKPFLGIVTAQVASATRFSLHSVDILL
                     AREGTPEASSAPPNRTRERLLAFTLEPRIAAPGEKVTFTARFRLPDDGVRYSSGCYTP
                     NRIFRVDEFEMRPVGKPDSETGTRTFQATLEVPKRPREEWAVFRLWLDRDGRYQEVEL
                     PRRRDLLLQIAQTPEERAAAQLASHAFDLEQAGRLADALQHYRRAAEALPERASLWAR
                     IGHLSLRLNRPAESIAAFEKLIAADPNHPETTTSHYAMALLAAGETQRALDALSEVEK
                     KSARRGQLPREFLLAMARAHLARSDLQTADRYYARAVKSGRVPNEVAQQFHLKRARAA
                     VKEMPENPDARAGLARALFDLGRYEEAAAAYRQALRLGPAQAWGHLDLAICLTAAGKD
                     RDALPHYRLAVELAPENLEACLALAECHRRLGEFSPALEAYRKVAAAPARRYDYRVQH
                     GLALALLATGQEASAMEALARAVTLGRSKGRLLGTRLPTGVGTLSLTPKRVAIDAFEH
                     PEMALDYALLNSLRVLRQAPDSSPALFQAGAALIELGVAEKGLEMLERALEHGFAPPD
                     AQYARAMACLRLERRAEARELLERLTRENPGHRRAWLALARIHFDAGNSPAGKACLLR
                     AQAEQVENR"
     gene            complement(5268..5987)
                     /gene="lptB"
                     /locus_tag="PLY56_03955"
     CDS             complement(5268..5987)
                     /gene="lptB"
                     /locus_tag="PLY56_03955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOJ20663.1"
                     /translation="MQITTDELVKTYRGRNVVDGVSLSVGKGEIVGLLGRNGAGKTTT
                     FYMVVGLVRPRRGRVMLGDRDITRLPMYQRARAGIGYLAQEASVFRKLTVEQNLRLVM
                     EMNRLSRAEQDKRIDQLLEEFTITHLRKEMGFSLSGGERRRVEIARCLATNPTFILLD
                     EPFTGIDPIVVSDIQEIIRHLKNRGIGILITDHSVRETLAITDRAYIIVEGKIRTAGT
                     PEQILNDPVAKEHYLGNHFAM"
     gene            complement(6074..>6515)
                     /locus_tag="PLY56_03960"
     CDS             complement(6074..>6515)
                     /locus_tag="PLY56_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20664.1"
                     /translation="QIGFKTRGGKVLLNFAKNVRATAQGAGQSVELHHADQATAEVDS
                     KWQFDTLKATGNVRFTISRKNGEQLEKTTARCNQVTIYNRFAQELTGDQRVLIAELSG
                     GVHLTMPAPKAAGATMEPMTLEGEHGQVWRTAEGYEGTISAQGD"
BASE COUNT         1057 a         2178 c         2186 g         1094 t
ORIGIN      
        1 cggtggtgtc gcccgcggga agcatcagcc agacgatgcg cggcgccgag agcttgccgg
       61 cgaaatcggc tagtgagtcc gagggcaccg ccccaaaggc ggcggcggcc tccaccggcc
      121 cgcgactccg gttgaacacc acgacgcggt ggcccccgtt gaccagacgc ctggccatgt
      181 tcgcgcccat gcggcccagc ccgtagatcc cgacttccat cgttcacctc gctctttctg
      241 caaacgtcca gaagcccgat gcctcagtat accacagccg cggcccgctc gtcacgccgc
      301 cgttccgcgg acgtgcgccc ggctcgggca ggctccagaa aggatggggt ggcctcgaaa
      361 gggaaccctt cacacggcgg atgcatccat agtatgggca tgaagtgcgg cgtgcgcgct
      421 gatgcccgtt cagcggctct tgccgtttca ctttgaatac cagagatttg cggatcgctc
      481 atggttgtcg atattcatac tcacgcgttt cccaacgacc ttgccccgcg tgccgtgaaa
      541 aagctctccg aagtggcgcg gatcccagcg cgcaccgatg gcacctgcga aggcctgcgt
      601 acctcgatgc gccgggccgg cgtggatctc tccgtgatca tgccgattgc caccaagccc
      661 actcaggtgc gcacgatcaa cgcctgggcg gcggaggtca acgccaccta tgaggatctc
      721 ctctcctttg ggacgcttca cccgctccag gaggattggg cggaggagat cgagcgtctg
      781 gtggccgatg ggatccgggg cgtcaagctg cacccggatt accaggagtt cttcgtcgat
      841 gacgcggcga tgattccgat ctaccgggcg cttgccaagg cgaggcgcat cctgctcctc
      901 cactccggcg tcgatatcgg gattcctccc ccagttcact gcacgcccga ccgcttggcg
      961 cgcgtgctag atgccgtgcc ggagctcacc gtgatcgcgg cgcacatggg aggctatctg
     1021 cggtgggagg ccgtggagca gttcctcatc gggcgcgacc tttacctgga tgcatcctac
     1081 tcgctggcag atcttggccc cgacgccctg gtgaggctgg cgcgcgccca cgggatcgaa
     1141 cgcgtgcttt ttggaaccga ttcgccctgg acagaccagg cgaccgagct ggccgccttc
     1201 cgcgatctcc cgctgagcga ggaggagaag gcagccctcc tcgggggcaa cgcccggcgc
     1261 ctcctcgggc tgagcgccgg ctgaaagggt gagtgagggc ctgcaggccg cccaggcgcc
     1321 cgccgagcca tcctctcgtg ggtctgcaga ccctcacaga cggaagttca ggtatccgtg
     1381 ctcaccttcg tggtagccag gtgcgcggtc acgcgcctgc taccatggaa gcgcgcagga
     1441 gcccgaatcg cgcgtctacc gtaggggtcc gcttcagaag cgaggggcag aactgcaaac
     1501 gaccaggctc tggctgttcc gcctgtatga tagcaggcca ccgtcacaga atcgtcacag
     1561 attcccccga gagggctacg aatccgccgg atcggaggtc cccacccgtg cctgccctgc
     1621 tcgattcgcc cgcagcgccg cgcggtagct caccagctcc tgagtgaggc cctggtgatg
     1681 ccacacggct ttcaccaggc cgaccccctc ggcaaaccag ccctccgcga tgagagcgcc
     1741 cttcgcatcg gtctgaatca cgcggacgca ctcctggtcg ccagcaggca cggtgatctt
     1801 ctcgagcggc cgcgtttccc ctagggattc ccacgaccac tcggcctgcc atggcccctc
     1861 gcttccctcg cccgccgggt gggtgagaat gcccgaggcc gttgtggtca cccacgcctg
     1921 cgtggagaaa ccgcctgggt actccgtcac cacgcgaaag atcccctcgg gccgggcggg
     1981 gtgcacctcg accacgcgcg tgaccgcatt gacctcgctt ccatcgcgcg cgcgaagccg
     2041 gtaggtccac tcatcgccaa tggccagcgg gaaccggggc gtaggcaccg agggcggcgg
     2101 catggaccag actggatccg ggatgggaag cgccgtgatc tggggccgat cgggcggtgc
     2161 tgcatcgagt tcggcagcca gggcctccag cacgcgcttc cggcggtgcg tgttggggtg
     2221 gctcatgagg aactccgggt gccggctacg ttcggcgagc tggagcagcg tctccatcgc
     2281 cttgagcccg ccctggcgat caaagccggc acgcacggtg tagaccagcc cctctcggtc
     2341 ggcttgaagc tcgtctgctc gggagaagcg ccggctgagg ataacgtggg tcaggtctcg
     2401 ccctagggcg ccgaggagac tcccaagggg agcggtgacc acgcggagga tctgcgactt
     2461 ctcgaactgg cgcagtgcgt gccggtgcac ggcgtgagcg atctcgtggg ccaggatgaa
     2521 cgcgagcgca tcgtcatccg gaaccccctg caacaggccc cggaagacgt agacgaagcc
     2581 gccaggaagg cagaacgcgt tgatgtcggg ggtatcgatc accttgaagc tgaaggggag
     2641 atccggacgc ccgcacacgc cggcaatacg ccgtccaatc cgctgcacgc gtgccgccag
     2701 aatcgcgtct gtggagatgg gatactgctc ttcgatgatc tgcgcagcct cgcgcccgat
     2761 gctgatctcc gcgtccaggg gaaggatgtc ggctgaggca ggcatgccgt gccacaatac
     2821 cgctcccagc aagactacca cggcgacgag gggtctgatc cggttcatcg gttttccacc
     2881 tgctccgcct gcgcgcgcaa caggcaggcc ttccctgcgg ggctgttccc ggcatcaaag
     2941 tggatgcggg ccagtgccag ccaggcccga cggtgccccg ggttctcgcg cgtcagtctc
     3001 tccagcagct cgcgcgcttc cgcccggcgc tccaggcgca ggcaagccat cgcccgggca
     3061 tactgggcat cggggggagc aaatccgtgc tcaagcgcgc gttccagcat ctccaggccc
     3121 ttttcggcaa cgcctagctc gatgagcgcc gcgcctgcct gaaacagcgc gggcgagctg
     3181 tcgggagcct gccgcagaac gcgcaagctg ttcagcaggg catagtcgag cgccatctcc
     3241 gggtgctcga aggcgtcgat ggccacccgc ttcggcgtga gcgagagcgt gccaaccccg
     3301 gtggggagac gcgtgccgag cagacgcccc ttggagcggc caagagtgac ggcgcgtgcc
     3361 agggcttcca ttgccgacgc ctcctgaccg gttgccagga gcgcgagcgc cagaccgtgt
     3421 tggacacggt agtcgtagcg gcgggcgggc gcggctgcaa ccttccggta ggcctccagg
     3481 gcgggggaaa actccccgag ccgccggtgg cattcggcaa gggcgaggca ggcctccagg
     3541 ttctccgggg ccagctcaac cgccaggcga tagtggggga gggcgtcgcg atccttgcct
     3601 gcggcagtga ggcagatcgc tagatccagg tggccccagg cctgggcggg gccaagccgc
     3661 agtgcctggc gataggcggc ggcagcctct tcgtagcgac ccaggtcgaa gagcgcccgg
     3721 gccagtccgg cgcgggcatc ggggttctct ggcatctcct tcacggcggc tcgtgcccgc
     3781 ttcagatgga actgctgggc gacctcgttt ggcacccgcc cgctctttac cgcgcgggcg
     3841 taatagcggt cggcggtctg tagatccgag cgcgccaggt gggcgcgcgc catcgccagg
     3901 aggaattcac gtggcagctg acctcgccgg gcgctcttct tctccacctc ggagagcgcg
     3961 tcgagggcgc gctgagtctc gccggcggcc aggagggcca tggcgtagtg cgaggtggtc
     4021 gtctccgggt gattcgggtc ggctgcaatc agcttctcga aggcggcaat cgattcggcg
     4081 ggccggttga gccgaaggga gaggtggccg atccgcgccc agagggaggc gcgctccggc
     4141 agggcttccg ccgcgcggcg atagtgttgc agggcgtccg ccagccggcc ggcctgctcc
     4201 aagtcgaacg cgtggctggc taactgggct gcggcgcgct cctcgggtgt ctgcgcgatc
     4261 tgcagcaaca ggtcccggcg ccgcgggagc tccacctcct gatagcgccc gtcgcggtcg
     4321 agccagaggc ggaaaaccgc ccactcctca cgcggacgct tcggaacctc cagcgtggcc
     4381 tggaaggtgc gagtccccgt ctccgaatcg ggttttccga caggtcgcat ctcgaactca
     4441 tccacgcgga agatcctatt tggggtgtag catccggagg agtagcggac gccgtcatcg
     4501 ggcagtcgga agcgtgccgt gaaggtgacc ttctcgccag gcgcggcgat ccgtggctcc
     4561 agcgtgaagg cgagcaaccg ctctcgcgtg cggttgggcg gcgccgatga tgcctcgggt
     4621 gtgccctcgc gcgcgaggag gatatccacc gaatggagcg agaaacgcgt ggccgaagcg
     4681 acctgagctg tcacgatgcc gaggaacggc ttatagccag gcgcatccac ctgcatcagg
     4741 cattggcgcg cgtccaccac gcgcgtctgc ttaaagaggc cccacggcat caagagccca
     4801 atcgggaaac cttcgaccag actcatcacg tccaggtgcg atgacttccg cccgatgcgc
     4861 gcgcgcacct gatagcggcc ttcggcgttt gtctggcccg tcgtgggccc ccgatccgca
     4921 aaggcgcccc cttcctcgac gacgacgcgc gcgccagcaa caggctcgcc cgtcgcccca
     4981 tcgaggacgt agccggttac cctgccccag tctcgggcga tggcgagacg ggtttcgctt
     5041 gccgccagcg ccccgccggg cgcggcaagc agcgcaacac agagacacac cgcaatcgac
     5101 cgccgatagc tcatgcgcac cgcagctttc tacgaaaccg ccacaagatc ttgccggaag
     5161 cctatgctga ggacgctttg cacgcgaagt aagttcaatc ggcgtctggc tgatctgggc
     5221 ggaaagcccg gcgcgggggc gcttcctccg gcggacagct ggaggggcta catcgcgaag
     5281 tggttgccca ggtagtgctc cttggcaacg gggtcgttca ggatctgctc cggcgtgccg
     5341 gcagtgcgga tcttcccttc gacgatgata taggcgcggt cggtgatggc gagcgtctcg
     5401 cggacgctgt ggtcggtgat caagatgccg atgccgcggt tcttcagatg gcggatgatc
     5461 tcctggatat cggagacgac aatcggatcg atgccggtga aaggctcgtc gaggaggatg
     5521 aaggtgggat tggtggccag gcaacgcgcg atctccacgc gccggcgctc gccgcccgag
     5581 agggagaagc ccatctcctt gcgcaggtgg gtgatcgtga actcctccag gagctggtcg
     5641 atgcgcttgt cctgctcggc gcgcgagagg cggttcatct ccatgaccag gcgcaggttc
     5701 tgctcgacgg tgagcttgcg gaagacggac gcctcctggg ccagatagcc gattccggca
     5761 cgggcgcgct ggtacatcgg caggcgcgtg atgtcgcggt caccgagcat cacacgcccg
     5821 cggcgcgggc gcaccagacc gacgaccatg tagaacgtcg tcgtcttccc ggcgccgttg
     5881 cgcccgagga ggccgacgat ctcccccttg ccaaccgaga ggctcacgcc atcaacgaca
     5941 ttgcgcccgc ggtacgtctt gacgagctca tccgtggtga tctgcacccc tgtgtcaccc
     6001 ttcccctggt cggggctctg gcacgagccg aagcccctcg cgcccgcgtg atgaagcgcg
     6061 agcgcgcgct acttcagtcg ccctgggccg aaatggtccc ctcatagccc tcggcggtgc
     6121 gccatacctg gccgtgctcg ccttccagcg tcatcggctc catggtggcg cccgccgcct
     6181 tcggcgccgg cattgtcagg tgcacgccgc cagatagctc cgcgatgaga acgcgctggt
     6241 cgccagtcag ctcctgggca aaccggttat agatcgtcac ctggttgcac cgggccgtgg
     6301 tcttttccag ttgttcgccg ttcttgcgcg aaatggtgaa gcggacgttc ccggtcgcct
     6361 tcagcgtgtc gaactgccat ttggagtcca cttcggcggt ggcctgatcg gcgtggtgca
     6421 gctcgaccga ttgccccgcg ccctgcgccg tcgcgcggac gttcttggca aagttgagta
     6481 ggaccttgcc gccgcgcgtc ttgaagccga tctgc
//
LOCUS       DAOSCV010000106         6512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7569_length_6512_cov_3.947189, whole genome shotgun sequence.
ACCESSION   DAOSCV010000106 DAOSCV010000000
VERSION     DAOSCV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7569_length_6512_cov_3.947189"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLY56_03965"
     CDS             <1..810
                     /locus_tag="PLY56_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20665.1"
                     /translation="DPEGGHLFKATVSGLPGDVIQEWGFPRVSFERLAAPVPGEGILR
                     LVASRDSERLFGLTNQTGTIFAYDLRERQARVLGQVDPIHFFSPVLVEDREGVWYAFG
                     SAGRLMRFSPFMETIEATDLRVECFPGRGPYAKIAAAALDDASRRLYLGDTEGLLSYV
                     DLDEWRVVPLGKPVALGGIDHLVLLPDGRLYGTAGSREGMAHLFVYQPRPGALRDLGV
                     LCATTEKPWYGYRYGDMVATPEGRIVLGEADHLGCLFNYFPPLLAAGNPIE"
     gene            1312..2157
                     /locus_tag="PLY56_03970"
     CDS             1312..2157
                     /locus_tag="PLY56_03970"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOJ20666.1"
                     /translation="MSRFQHVAAKLLLPVCALCLLGLDFGRAQDAGSSPARQGGTIAS
                     SVNIEVDGQVLAVHMAGGDVAEALKVAGVELGEEDECSPAPGEAIAPNMTITVRRVCT
                     RTVVQQEPIRARTRVIPTRQLRAGITCIKQHPEDGLKELTYRVTWRDGEVASRELLDA
                     RVVRPVKDKLILVGGGGQKLPSRGGYFSGRRVMRMVATGYPAMVTGTGRTYLGLKAER
                     GIVAVDPRVIPLGARVHVEGYGEALAADIGSAIKGNRIDLCFPTHREASRYGRKVVTV
                     RILTP"
     gene            2289..3170
                     /gene="rsmA"
                     /locus_tag="PLY56_03975"
     CDS             2289..3170
                     /gene="rsmA"
                     /locus_tag="PLY56_03975"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010279419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ20667.1"
                     /translation="MRATEAMNLTSPHVVRELLRRHGVTLHRALGQNFLVDANILRKI
                     LDAAELSPETGVLEIGAGIGTLTRALAEHARHVLVIELDRSLRPILRETLAGVENVHV
                     EYGDFLKVNLPALLDAYCAPLCQVVANIPYQITSPLITRLLEHKASFTRMVLMMQKEV
                     AERLLAKPGTGEYGSMTVFVRYHAEVRLVTHVSRNSFLPPPKVDSAVVRLDPRAEPPL
                     PVKDEKRFFAVVHAAFGARRKTLLNALSGGLGQPREVLEPALERAGIDPKRRGETLSL
                     EEFITLSNELHGDLMEA"
     gene            3175..3708
                     /locus_tag="PLY56_03980"
     CDS             3175..3708
                     /locus_tag="PLY56_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ20668.1"
                     /translation="MKVGIVSDTHDHLENLAEAIRLLNARGVELVLHLGDWVAPFVPV
                     FMTKTAPEPLRCPVKGIFGNNDGDHFLFLQVIAREQTGIEIRKTLLPLELDGRRIVCY
                     HGTEPEITEALVASKRWDVVFAGHTHEAGTREVDGVLHVNPGSLSGIRGGKPGGPATF
                     ALYDTATGEAEIITLGE"
     gene            complement(3838..5511)
                     /locus_tag="PLY56_03985"
     CDS             complement(3838..5511)
                     /locus_tag="PLY56_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOJ20669.1"
                     /translation="MLARLRGPAISSHQRLRTTQGVFSLRRGGLLRGYAGLIAGVPLT
                     FSVGGNPSHPGAGGNAFPIVKPPEAMRLTIKEPPSGTKSIKIERDPSTPATDKEQTPA
                     PSSEAAPATEAPALKPTVQPPTAAAPSVEPEPPAATQAAEMGAAPRATAPPGDPSETV
                     QAATPDPDPVAAKAADDTPAPQVAAESAPPAAGTPTESKPVAKTAAKAEPAIQPAAAE
                     TKTQAAADSPPNPETATAQAAAEAKPVAETQPETASKAKPEAAAKTVAKAEPAPAAKP
                     KTVAKAEPQSVDKPTVKAEPKTAAQPKTTAKAVAKAEPKPAAKTTAKAQSKTAAKPAT
                     TAQSKTTAKTAAKPKTVAKAAPKPAAKTANADPKPAAKTVAKAEPKPAAKTAAKAQSK
                     TAAKPVTKAKPETAAKPKAVAKAQPKAAQPLASRGGSGTVQNIHRIIEAAAKEFGVDP
                     ALVRAVVRAESGFNPRALSRVGAMGLMQLMPATARALGVTNAWDPVQNIRAGTRFLRS
                     LLDQFKNERLAIAAYNAGPGAVKRHGGIPPYRETQDYVKKVLSFRNNGG"
     gene            5870..>6512
                     /locus_tag="PLY56_03990"
     CDS             5870..>6512
                     /locus_tag="PLY56_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20670.1"
                     /translation="MQVLRLLTILLPVLMILPGRDPLAADRALVPAPAPATLSTPQGT
                     PEPKSITVDPPAPRAALVPARGEVVVEGVIDAVVKDHRGLQVSIFRVAAPSGAEERPK
                     PLLSGTLLLGIASLITPLGPDGQRVPADALRPGTLFTAVIEWPASPSPSVLAVRRLTV
                     NVPEASTGAAPASGENSLGAIAPDRISDAPVIVPMVFPVVGPVSWQDNFLASRG"
BASE COUNT          959 a         2082 c         2255 g         1216 t
ORIGIN      
        1 gatcccgaag gcggccacct cttcaaggcg acggtgagcg gccttccagg cgatgtcatt
       61 caggagtggg gctttccccg ggtgagcttc gagcgcctcg ccgcgccggt gccgggcgaa
      121 gggatcctcc gcctggtggc ctctcgcgac agcgagcgcc tcttcgggct gacaaaccag
      181 accggaacca tctttgccta cgatctgaga gagcgccagg cgcgcgtcct gggccaggtg
      241 gacccgattc acttcttcag ccccgtgctc gtcgaggatc gcgagggcgt gtggtatgcc
      301 ttcggctcag cggggcgcct gatgcgtttc tccccgttca tggaaacgat tgaggcgacc
      361 gatttgcgcg tggagtgctt cccgggccgc gggccctacg cgaagatcgc cgcggccgcg
      421 ctcgatgacg cctcccgccg gctctacctg ggcgataccg agggcctcct ctcctacgtg
      481 gatctcgacg agtggcgcgt agtgccgctg ggcaagccgg tggcgctcgg agggatcgac
      541 cacctggttc ttttgcccga tggccgtctc tatggcaccg ccggcagccg tgaggggatg
      601 gcccacctct tcgtctacca gccgcggcct ggggcgctac gcgacctggg cgtgctgtgc
      661 gccaccacgg agaagccctg gtacggctac cggtatggcg atatggtggc cacgcccgag
      721 ggccgtatcg ttctgggtga agccgatcac ctcggttgcc tcttcaacta cttccctcct
      781 ctcctcgccg cggggaaccc catcgaataa gccggacctg gccaggttga acccgcatcc
      841 cacggagacg ggcgcggagg ggaagaccct gcccgtgccc gtccccaggg agggcgattg
      901 gctgcctgcc atggcgaggc cgcgctctgc ctgtggcaaa gggtggcaaa cggctctctt
      961 gcgcgcggat gtgtagaagc cggtcgatgt gggtaacatg gactgacact gcgcccgaac
     1021 cgcgaagagg agcaggcccg ggagggaaga cgtcccaagg cttcccacgc tatcccacgc
     1081 cgtacccttg cctgccactg gcgggccgtt tctccccacg ggctcccaca gtagcgcgaa
     1141 aaatggcgca ttgctccctg tagaaaagca agcagtacgg gcgcttgtgt gccggtgtgc
     1201 ccgagggtgc ggttgacata tcacgctttt tggtgtacaa tcgcgctcac gtcggaccgg
     1261 cgggataccg ccggtctggc aggcaccgaa cgcggaagga ggaaagggca gatgagccgt
     1321 tttcagcacg tcgctgcaaa gctgcttctc cccgtatgcg cgctgtgcct gctgggtctg
     1381 gatttcggcc gggcccagga cgcagggagt tcccccgcaa gacagggggg gactatcgca
     1441 tcatcggtga acattgaagt ggatggccag gtcctggctg ttcatatggc aggaggggat
     1501 gtcgccgagg cgctgaaagt agccggagtg gagcttggcg aggaagatga atgctcgccg
     1561 gcgcccggcg aagcgattgc gccgaacatg acgatcaccg tgcgccgcgt gtgcacgcgc
     1621 acggtagtgc agcaagagcc gattcgtgcg cgcacccggg tgattccgac gcgccagctg
     1681 cgcgcgggga ttacctgcat caaacagcac ccggaggatg gtctgaagga gctgacttac
     1741 cgcgtcacct ggcgtgatgg cgaggtggcc tcacgcgagc tgcttgacgc gcgagtggtc
     1801 cggcccgtga aggacaaact catcctcgtg ggtggggggg gccagaagct gccctcccgc
     1861 ggcggctact tcagtggccg gcgtgtcatg agaatggtgg cgaccggcta ccccgccatg
     1921 gtgaccggca cgggccgcac ctacctgggc ctgaaagcag agcgaggtat tgtggcggtc
     1981 gacccgcggg tgatcccact gggtgctcgc gtgcatgtag agggctatgg cgaggcgctc
     2041 gccgccgata tcggcagcgc catcaagggt aaccggatcg acctgtgctt cccgacccat
     2101 cgggaggcaa gccgctatgg cagaaaggtg gtgacggtgc gcatcttgac cccgtagcca
     2161 ctcgctgcgc ggctgggttt ggagacaggg aagcatgaga agagagatcc accgttcaag
     2221 gcgaacgcgc cttcataggg ggcccctccc ccccaaggct ctcccgtttt cctgtctcca
     2281 aacgatgcat gcgggccact gaagcgatga acctcacttc tccccacgtc gtgcgcgagc
     2341 tcctgcgccg gcacggcgtc acccttcacc gggcgctagg ccagaacttc ctcgtcgatg
     2401 ccaacatcct gcggaagatc ctcgatgccg cggagctgtc tcctgagacg ggcgtgctgg
     2461 agatcggagc ggggatcggc acgctgacgc gcgccctggc ggagcatgcc cggcacgtgc
     2521 tcgtcatcga gctggatcgc tcgttgcggc cgatcttgcg agagactctc gccggtgtgg
     2581 agaatgtgca tgtggagtac ggcgatttcc tgaaggtgaa tctgccggcg ctcctggatg
     2641 catactgtgc gcccctgtgc caggtggtgg ccaatatccc ctaccagatc acctctccgc
     2701 tcatcacgcg tttgttggag cacaaggcgt ccttcacccg gatggtgctg atgatgcaga
     2761 aggaggtggc cgagcgcctc ctggcaaagc cgggcactgg ggagtacggg tcgatgaccg
     2821 tctttgtgcg ctaccatgcc gaggtgcgcc tggtgacgca cgtctcgcgc aactcttttc
     2881 tccccccgcc aaaggtcgat tccgccgtgg tgcgcctgga cccacgcgca gagccgccgc
     2941 tgccggtaaa agatgagaag cgcttctttg ccgtcgtcca cgcggcgttt ggcgcgcgcc
     3001 gcaagacgct gctgaacgcg ctttccgggg gattgggtca accgcgcgaa gtgctggagc
     3061 ctgcactaga gcgcgctggc attgatccga agcgccgcgg ggagacgctc tcgctcgagg
     3121 agttcatcac cctgagcaat gaacttcatg gcgatttgat ggaggcgtga cgcgatgaag
     3181 gtaggcatcg tttcggatac ccacgaccac ctggagaacc tggcggaggc gatccggttg
     3241 ctcaacgccc gaggcgtgga gctcgtgctc catctgggcg actgggtggc gccgttcgtg
     3301 ccggtcttca tgacgaagac ggcgccggag ccgctgcgct gcccggtaaa agggatcttc
     3361 gggaataacg atggcgacca tttcctcttt ctccaggtga tcgcccgcga gcagaccggg
     3421 atcgagatcc gcaagacgct cctgccgctg gagctggatg gccggaggat cgtctgctac
     3481 catggcaccg agccggagat caccgaggcg ctggtggcga gcaagcgatg ggacgttgtc
     3541 ttcgccgggc acacgcacga ggcggggacg cgcgaagtgg acggcgtgtt gcacgtaaac
     3601 cccgggtcgc tgagcgggat tcggggcgga aagccagggg ggccggctac cttcgccctc
     3661 tacgatacgg ccactggcga ggcggagatc atcacgctgg gcgagtaggc gcgcctgctg
     3721 ggataccttc cggatgtgcg agggacgcca gggagagagt atgctggcgc ccgtggaaaa
     3781 acgaagggcc cggcgccaag cgccaggccc atcacgagaa ggatgcgctt cggagactca
     3841 cccgccgttg ttacggaagg agagcacctt cttcacgtag tcctgcgtct cacggtaggg
     3901 tgggatacct ccgtgccgct tcacggcgcc cggccccgcg ttgtaggcgg cgattgccag
     3961 gcgctcgttc ttgaactgat ccagcagcga gcgcaggaag cgcgtgccgg cgcggatgtt
     4021 ctgaaccgga tcccaggcgt tggtgactcc gagggcgcgc gccgtggccg gcatgagctg
     4081 catcaggccc atcgcgccca ctcgggagag tgcccggggg ttgaacccgg actccgcgcg
     4141 caccacggcg cgcaccagcg ccggatcgac gccgaactcc ttggccgctg cctcgatgat
     4201 gcggtggatg ttctggaccg ttccggatcc cccgcgcgag gcgagcggtt gcgccgcctt
     4261 gggctgagcc ttcgcgacag ccttcggctt cgccgcggtc tctggcttcg cctttgtgac
     4321 gggctttgcc gccgtcttgg attgtgcctt cgccgccgtc ttagctgccg gcttaggctc
     4381 cgcctttgcg accgtcttgg ctgcgggctt aggatccgcg tttgccgtct tagccgcggg
     4441 cttaggcgcc gccttcgcga cagtcttggg cttggccgca gtcttggctg tggtcttgga
     4501 ctgtgccgtt gtggctggtt tggctgcggt cttggattgt gccttcgccg tcgtcttggc
     4561 cgcgggttta ggttccgcct ttgcgaccgc cttggctgtg gtcttgggct gtgccgcagt
     4621 cttaggctcc gctttcaccg tcggcttgtc caccgactga ggttctgcct tcgcgacagt
     4681 cttgggcttc gccgctggcg caggctctgc tttcgcgacc gtcttggctg cggcctcagg
     4741 cttcgccttc gacgcggtct cgggctgagt ctccgccacg ggcttcgctt ccgccgctgc
     4801 ttgggcggtg gcggtttccg ggttcggcgg cgagtccgcc gcggcctgcg tctttgtctc
     4861 agcagcggca ggctggatgg ccggctccgc tttcgcagcg gtctttgcga ccggcttcga
     4921 ttcagtcggc gttccagcgg ccggtggtgc gctctctgcc gcgacttgtg gtgccggggt
     4981 atcatctgcc gctttggctg ccacgggatc cgggtctgga gtggcagcct ggacggtctc
     5041 cgacggatcg cccggcggcg cggtcgcgcg gggcgcggca cccatctccg ctgcctgcgt
     5101 ggccgcgggc ggttccggct cgacggaggg cgcggccgcc gtggggggct gcaccgtcgg
     5161 cttgagcgcg ggcgcctccg tggcgggcgc cgcctcggat gatggcgccg gcgtctgttc
     5221 cttatccgtg gccggggttg acgggtcgcg ctcaatcttg atgctcttcg tgccggacgg
     5281 cggctctttg atggtcaggc gcatcgcctc gggcggcttc acgatgggaa aggcatttcc
     5341 gcctgcccca ggatgcgagg ggttgccccc gaccgagaag gtgagcggaa cgcctgcgat
     5401 gagcccggca tatcctcgga gcaggccgcc gcgccgcaaa gagaagacac cctgggtggt
     5461 tcggagccgc tggtggctgc ttatggcagg cccgcgcagg cgggccagca tccgaatcaa
     5521 caacggttcc cctttccggt aggtcgctcg aagggcacgc gcgggggctg accgattcga
     5581 gcccgccacg gcgccaatcg cagaatcaag aagggcgctt tgccgtgccc catggggctt
     5641 aggacagaga aatgggtgtt gaggttgcct gccgctctcc acacggaatt cgcctgggcg
     5701 cgacgggcga ctatcgctcc tgaccagcta cgggaatcgc gattgctccg aattctgcgt
     5761 gccggcatcc ccgccggggg atctctcggc ccacacgggc gtgcgagtga cgcgctctcc
     5821 ggtgaaccac gggcgtggtg caagcgtctt gcaatccgag gtattccaca tgcaagtgct
     5881 ccgccttctc accatcctgt tgccggtctt gatgattctt cctggccgcg acccgctggc
     5941 ggccgaccgt gccctggttc cagccccggc gccggccacg ctttccacgc cgcaggggac
     6001 cccggagccg aagagtatca cggtggatcc cccggcgcct cgcgccgccc tggtgcccgc
     6061 gcggggcgag gtggtcgtcg agggtgtcat cgatgccgtg gtgaaggatc accgcgggct
     6121 gcaggtgagc atcttccgtg tcgctgcgcc gagcggtgcc gaggagcgcc cgaaaccgct
     6181 tctttccggc acgctgctcc tcgggatagc gtctctgatc acgccgttgg ggccggacgg
     6241 ccagcgtgtt cctgccgatg cgttgcggcc tggcacgctt ttcaccgcgg tgattgagtg
     6301 gccggcgtcg ccctccccgt ccgttctcgc ggtgcgccgg ctcacggtca atgtaccgga
     6361 ggcgtcgacc ggcgccgccc cggcctccgg tgagaattca ctgggagcaa tcgcgccgga
     6421 ccggatcagc gacgcgcccg tgatcgtgcc gatggtcttc cccgtggtcg gcccggtttc
     6481 gtggcaggac aatttcctcg cctcgcgggg cg
//
LOCUS       DAOSCV010000107         6497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7594_length_6497_cov_3.541292, whole genome shotgun sequence.
ACCESSION   DAOSCV010000107 DAOSCV010000000
VERSION     DAOSCV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7594_length_6497_cov_3.541292"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLY56_03995"
     CDS             <1..474
                     /locus_tag="PLY56_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20671.1"
                     /translation="PIRLAFMDEAHPLADGLSEKQEFGPHANLKFGPLPRVTDPKAQV
                     IGRFVPDGPPAASVRQHEGWMSVYLAAPVASPVSLRNLARLAGLHVYLDTNDAVYASG
                     SLVGVHTQSAGEKTIRLPVKATVTDLFTGKVIAKGVDHFTVTLPARATALWRIEH"
     gene            complement(638..1453)
                     /locus_tag="PLY56_04000"
     CDS             complement(638..1453)
                     /locus_tag="PLY56_04000"
                     /inference="COORDINATES: protein motif:HMM:NF037749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HOJ20672.1"
                     /translation="MPRLMKHTLWAGLLGFCAALLLRLPVDTVSAGNAARTRFGEARR
                     VGQGRARSWVTLDARGRPLALGVEITDQALSGLPSHKEEYVLSLPAGVAVPPFNHTAL
                     DWNPHGHIPPQIYDVPHFDFHFYFMSPAERARITAKGDDVARVRRAPPADAIPAGYMY
                     APGGDEPAMGAHWVNPQSPEFHGKPFTHTLIYGFYDGRMVFVEPMVALSFLQTRPCVT
                     AAIPQPKSYPARGYYPTRYQIRHVTSANGGAIRVSLEGLVLRDGPPTASRGGR"
     gene            complement(1772..2995)
                     /locus_tag="PLY56_04005"
     CDS             complement(1772..2995)
                     /locus_tag="PLY56_04005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ20673.1"
                     /translation="MNEKQRKDALKRGISLEWPPEPILGSYYGEEEVEAIVKTVRASM
                     DPRVGFGFICEEIWEFERAFAQWVGTEFCVSINGAGGGLDMAMMCLDLEPGDEVIVPS
                     INFRASPLAVLGQRATPIFAEIDPKTLNLDPNDVERKVTPRTRAIVPTHMNGLSADMD
                     ALEEIGRRHPHPKYGPIPVIGDAARTLGATYKGTKVGKKGWMNIFSFHTQKMITTLGE
                     GGAITTDDRDLATRLQGIRQFGNPAHSYDPKLRGIGWGSNYKMTKIQAAVGLVQIKRL
                     DDLVARRQRLARARTAMLEGCPGLQLPYEPEGYESSFYLYTILVPKEWAGEKRDQLMT
                     ILKEQYKVECVVANPPVHNTVPYIAEHAPGQNLPISDEVGARIFCPPIHPYMPDEDNE
                     YIAAAIWEAVLKLRD"
     gene            complement(3282..4253)
                     /locus_tag="PLY56_04010"
     CDS             complement(3282..4253)
                     /locus_tag="PLY56_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20674.1"
                     /translation="MKPKERAIAALELREPPEGLVPTFELEFQLTQELLGKEYHKGNA
                     WRGVTRAERDRMLHENAELLVEVGERLDYCILMDTCSPDVEGHIETARLIKELSGDRF
                     LVIVHGDATYAIPDGNHMVEYALWFAEKPDEAKRVADERVTAALERGRRLVDGGIDGF
                     ALCADYCLNSGPFLSPRMFSEFVTPFLARLVAGYREMGAYVIKHTDGNIMPILDQLVS
                     CRPHAIHSIDTQAAEMDLRVIKEKIGKEVCLIGGVQCGLLQTGTEEEIIANCRYALEH
                     GMPGGGYIYSTSNVAFKGLPLERYLLVLEMRQKYGWYGTPPDAAGAA"
     gene            4465..>6497
                     /locus_tag="PLY56_04015"
     CDS             4465..>6497
                     /locus_tag="PLY56_04015"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ20675.1"
                     /translation="MTLTTLSPRPGSVAFGRCTVEWTGEELALANTRIERRWRVANGL
                     LYPLSLKDRASGREWLARPAEQPAPCPEVPLPEEPRAIRVMAGHGVFGPTEAPALRVE
                     VVSTGPTLALCTCLQLFDDAPGIQVQLHAEPVEAPAGEARSGTASGEDAAPTGIEGPA
                     HEAGTPAGPVDLLEQLALSPAHLRLTQVTLLDQTDIRNELLFESEWLLHPNELRLELS
                     GNLFAIEELFSGDGLLFLKQAPLPHARPVQAGPDLRVNGASRVFHFLGHGAGPGGGQG
                     YPYVILVYRGGASGRTEALQAYQRQIRAFNPARDGLLLSNTWGDRSQDSRVCEEFMRQ
                     EVNAAASLGVDVIQIDDGWQKGRTSNSAQAGGVWGGFWAADPEFWAPHAERFPNGLEP
                     IIAAARERGMQFGLWYAPDPADDYANWERDAARVLELHRAHGIRTFKIDGVRMESKAA
                     ETNLRRFFDRVLEETRGEVVFDLDVTAAVRPGYWGMMHVGPLFIENRYTEWRRYWPHQ
                     TLRNLWKLARYVDPLRLRMEFLNHTRNTHLYGDDPLAPASYSPAYLFAITLFANPLGW
                     FEVSNLPAAYVEEVAELVKVWKAHRERLFQGYIHPIGETPDGTSWTGFLSVGEDRHEG
                     YLILFREVNDRPSYSLALPMLQAGEYAVECLAGQGSAVVHAGRVEAEIPNARG"
BASE COUNT         1056 a         2141 c         2151 g         1149 t
ORIGIN      
        1 ccgatccgcc tcgccttcat ggatgaggcg cacccgctgg ctgacggcct gagcgaaaag
       61 caggagtttg gcccgcacgc gaacctgaag tttggccctc tcccccgggt aaccgacccg
      121 aaggcgcaag tgatcgggcg cttcgtgccg gacggcccgc cggcggcgag cgtgcgccag
      181 cacgagggct ggatgagcgt ctacctcgcc gcgcccgtcg cctcgcctgt ctccctgcgc
      241 aacctcgcgc gcctggccgg gcttcacgtc tatctcgata cgaacgacgc cgtctatgcc
      301 tctggctcgc tcgtcggggt gcacacccaa tccgcgggag agaagacgat ccgccttccg
      361 gtgaaggcga cggtgacgga tctcttcacg gggaaggtga tcgcgaaggg ggtggaccac
      421 ttcacggtga cgcttcccgc gcgcgctacc gccctttggc ggattgagca ctagccgggg
      481 gcctgcttgg gcatcaggcg gtgtcggctg ggaatcgggt cacggctctt gcccgtggat
      541 aggtaacaaa gcgtcgcgcg gatccttgcg tccgcgcgac gcctgccggc atccccaggc
      601 gaggacgttc acgttcgcgt gctgtctgcc gggccggtca tcggcctccc cgggaagcgg
      661 ttggcgggcc gtcgcggaga accagacctt ccagggatac gcggatggca cccccgttgg
      721 cgctcgtgac gtgccgaatc tggtagcgtg ttgggtaata gccacgcgca ggatagctct
      781 ttggctgcgg tatcgctgcc gtaacgcatg gccttgtctg caggaaggac agcgcgacca
      841 tcggctcgac gaaaaccatt cgtccgtcat agaagccgta gatgagcgtg tgcgtaaagg
      901 gcttcccgtg gaactcgggc gattgcgggt tcacccagtg ggcgcccatc gcgggctcgt
      961 cgccaccagg agcgtacata tagcccgccg ggatggcgtc ggcgggtggc gctcgccgca
     1021 cgcgcgccac atcgtctcct ttcgccgtga tacgggcgcg ctctgccggg ctcatgaagt
     1081 agaaatggaa atcgaagtgg ggcacgtcgt agatctgtgg cggaatatgc ccgtgcggat
     1141 tccaatccag cgccgtatgg ttgaacggcg gcacggcaac cccggcgggc agagacaaca
     1201 catactcctc tttgtgtgac ggtagccccg agagcgcctg atcggtgatc tctacgccca
     1261 gtgctaacgg cctgcctcgg gcgtccaggg tgacccagga gcgtgcccgg ccctgaccga
     1321 cccgccgggc ctccccaaag cgcgtgcgtg ccgcgttgcc ggccgacacc gtatccaccg
     1381 gcagcctcag aagcagcgcg gcgcagaacc ccagaagacc cgcccacagg gtgtgtttca
     1441 tcagacgcgg catctcactc tcccatcgct cctgaccctt ccctgtggcc gcgcgcgcga
     1501 aacgccgctg ccaggccttt ccacacgcga gctggtaacg cccagggtct caaggcgcgg
     1561 cgcgccaagg cgcgatagcc gttatctcct tggcgggagt gccttgcgcg agcgcatgat
     1621 cgccctagga tggggcatgc gaaacacgag ggggggcagg agacgggcgt ctttctctca
     1681 ccgctggggc atgtgggctt ccgacgccac gaatactgtg cgggaagggg agcgttagaa
     1741 gcccgcttcc aacgctcccc gaggtgtcga ctcagtcgcg gagcttcagc accgcctccc
     1801 agatggcggc ggcgatgtac tcgttatcct cgtccggcat gtaggggtgg atcggcgggc
     1861 agaagatgcg ggcgccgacc tcatccgaaa ttgggaggtt ctggccgggg gcatgctcgg
     1921 cgatgtaggg gaccgtgttg tgcaccggcg gattggccac cacgcactcc accttgtact
     1981 gctccttcag gatcgtcatc agttgatccc gcttctcgcc ggcccactcc ttgggcacga
     2041 ggatcgtgta gaggtagaag ctgctctcat acccctccgg ctcgtagggg agctgcaggc
     2101 cggggcatcc ctccagcatc gccgtgcgcg cgcgcgcgag gcgctggcgc cgcgcgacca
     2161 gatcatccag gcgcttgatc tgcacgaggc ctaccgccgc ctgaatcttg gtcatcttgt
     2221 agttgctgcc ccaaccgatg ccgcgcagct tcgggtcgta cgagtgcgcc gggttgccaa
     2281 actggcgaat gccctgcagg cgggtggcca ggtcgcggtc atcggtggtg atcgcgccgc
     2341 cttcgcccag ggtggtgatc atcttctggg tatggaagct gaagatgttc atccagccct
     2401 tcttgcccac cttcgtgccc ttgtaggtcg cgccgagggt gcgcgcggca tcgccgatca
     2461 ccgggatggg gccgtacttc ggatgcgggt ggcgccggcc gatctcctcc aacgcgtcca
     2521 tatcggcgga gaggccgttc atatgggtgg ggacgatggc gcgcgtgcgc ggcgtgacct
     2581 tccgctccac gtcgttggga tccaggttca gcgtcttcgg gtcgatctcg gcgaagatcg
     2641 gcgtggcgcg ctggccaagc accgccagcg gcgaggcgcg gaagttgatc gagggcacga
     2701 tcacctcatc gcccggctcc aggtccaggc acatcatcgc catatcgagc ccgcctccgg
     2761 cgccgttgat cgagacgcag aactcggtgc ctacccactg ggcgaaggcc cgctcgaact
     2821 cccagatctc ctcgcagatg aagccaaagc ctacacgggg gtccatcgag gcgcggacgg
     2881 tctttacaat cgcctcgacc tcttcctcac cgtagtagct gccgaggatc ggctccggag
     2941 gccactccag tgagatgccc ctcttcaggg cgtccttgcg ctgcttctcg ttcatatccc
     3001 tctctcctaa tgccaatgat gatggcaagc tcgcccggcg cccgcgtgcc gaacatggtc
     3061 catgacgcca aaccaagcgg gcaacgagac cgagctatcg catctgcaag gggctgcgcc
     3121 gtttgccact cgatagggag cagcccttct gcctacctgc aaccggtagg agcaggaacc
     3181 gggcgacatg cccacggaag atgctgcgcc cgtatggatc aactaccacg cagccaccgg
     3241 aactcctgcc gacagaagcg cactgctggc gcggcggggg ctcacgccgc cccggcggcg
     3301 tccggaggcg tgccatacca tccgtacttc tgccgcatct cgagcaccag caggtagcgc
     3361 tccaggggaa ggcctttgaa ggcgacattg ctcgtgctgt agatgtaacc gccgcccggc
     3421 atgccgtgct ccagggcgta tctgcagttc gcgatgatct cctcctcggt gccggtttgc
     3481 aggagtccac actgcacccc gccaatgagg cagacctcct tgccgatctt ctccttgatc
     3541 acgcgcaggt ccatctccgc ggcctgggtg tcgatggagt gaatggcgtg gggccgacac
     3601 gaaacgagct ggtcgaggat cggcatgatg ttgccatcgg tgtgcttgat cacataggcg
     3661 cccatctccc ggtagcccgc caccagacgg gccaggaagg gagtgacgaa ctcggagaac
     3721 atccgcggcg agaggaacgg gccggagttg aggcagtaat cggcgcagag cgcgaagcca
     3781 tcgatgccgc catccaccag gcggcggcca cgctccaggg cggcagtgac gcgctcatca
     3841 gccacccgct tcgcttcatc cggtttctcg gcgaaccaga gggcatattc caccatgtga
     3901 ttgccgtcag gaatggcata ggtggcatcg ccatgcacga tcaccaggaa gcggtcgccc
     3961 gagagctctt tgatcaggcg cgccgtctcg atgtggccct ccacgtctgg cgagcaggtg
     4021 tccatgagga tgcagtaatc gagacgctcg ccgacctcca cgaggagttc agcgttctcg
     4081 tggagcatcc ggtcgcgctc ggcgcgcgtc acgccccgcc aggcattgcc cttgtgatac
     4141 tccttcccca ggagctcctg ggtgagctga aactccagct caaaggtggg taccagcccc
     4201 tcggggggct cgcgcaactc cagcgcggca attgctctct ctttcggctt catcgctttc
     4261 tcgctctcca cgcgccccac ggcgctggcg gctcctcgat accgtgcatg gatgcccgcc
     4321 catactctgg ctcgagcctc ttcacgctgc caatggaata ggccctgcct agaagttcag
     4381 gatagagcgc ggagatcctg ggagtctgcg cttcccgcgt cgcagcggga aggggtggcg
     4441 cggcccgggg cgaatccctc cgatatgaca ctcaccacgc tctcaccacg gccgggctcg
     4501 gtggccttcg gccgatgtac cgtcgaatgg accggagagg agctggcgct cgccaacacc
     4561 cggatcgagc ggcgatggcg ggtggccaat gggctcctct atccgctctc cctgaaggat
     4621 cgggcttccg gtcgggaatg gctggcgcgc cctgcggagc agccggcgcc ttgcccggag
     4681 gtgccgctcc cggaggagcc gcgcgcgatc cgcgtgatgg ccggccatgg cgtctttggc
     4741 ccgaccgagg cgcccgcgct ccgggtggag gtcgtctcca ccggccccac gcttgcgtta
     4801 tgcacctgcc tccagctgtt cgacgacgcg cccggcatcc aggtccagct ccacgcggaa
     4861 ccggtcgagg ctcccgccgg agaggcgcgc tctggcaccg cctcgggcga ggatgcggca
     4921 ccgacaggga tcgagggccc ggcacacgag gcgggcacgc ccgccggtcc ggtggatctc
     4981 ctggaacaac tcgcgctctc gccggcgcac ttgcgcctca ctcaggtgac gcttctggat
     5041 cagaccgata tccgaaacga gctcctcttc gagagtgagt ggctgctcca cccgaacgag
     5101 ctccggctgg agctgagcgg gaatctcttc gccatcgagg agcttttctc gggcgatggc
     5161 ctgctcttct tgaagcaggc tcctctgcca catgcccgtc cagtgcaagc ggggcccgat
     5221 ctgcgcgtca acggtgccag ccgcgtcttc cacttcctcg ggcacggggc cggacccggt
     5281 ggcggccagg gctatcccta cgtcatcctg gtctatcggg gtggtgcctc cgggcggacg
     5341 gaggcgctgc aggcctatca acgccagatc cgcgccttca accccgcgcg cgatggtctg
     5401 ctgctgagca acacctgggg cgaccgctcg caagactcgc gcgtctgcga ggagttcatg
     5461 cgccaggaag tgaacgcggc ggcatctctc ggcgtggatg tcattcagat cgatgatggc
     5521 tggcaaaagg gccggacgtc caactccgcg caggcgggtg gggtgtgggg tggcttctgg
     5581 gcggcagacc cggagttttg ggcgccgcac gcagagcgct tcccgaacgg cctggagccg
     5641 atcatcgccg cggcccggga acgggggatg cagttcgggc tctggtacgc gccggatccc
     5701 gcggatgact acgccaactg ggagcgggat gccgcgcgcg ttctggagct gcaccgggcg
     5761 catgggatac gcaccttcaa gattgacggc gtgaggatgg agagtaaggc cgcggagacc
     5821 aacctgcgcc gcttcttcga ccgcgtgctg gaggagacgc gtggcgaagt ggtctttgac
     5881 ctggatgtca ccgccgcggt ccggcctggc tactggggga tgatgcacgt ggggccgctc
     5941 ttcatcgaga atcgctacac ggagtggcgt cgctactggc cccaccagac gctgcgcaac
     6001 ctctggaagc tggcgcgcta tgtggacccg ctgcgcctgc gcatggagtt cctcaaccac
     6061 acgcggaaca cgcatctcta tggagatgat ccgctggcgc ctgcgtcgta ctcgcccgca
     6121 tatctcttcg ccatcaccct cttcgcgaac ccgctcgggt ggttcgaggt ctccaacctg
     6181 ccggcggcct atgttgaaga ggtggcagag ctggtgaagg tgtggaaggc gcatcgcgag
     6241 cgcctcttcc aggggtatat ccatccgatc ggcgagaccc ccgatggcac gtcgtggacc
     6301 ggcttcctct ccgtgggcga ggacaggcat gagggctacc tgatcctctt ccgcgaggtg
     6361 aacgaccgtc cgagctactc gctcgcgttg ccgatgctcc aggccggaga gtatgccgtc
     6421 gagtgcctgg cggggcaggg gagtgctgtc gtccatgctg gccgggtgga ggcagagatt
     6481 ccgaacgcgc ggggctt
//
LOCUS       DAOSCV010000108         6476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7618_length_6476_cov_3.261953, whole genome shotgun sequence.
ACCESSION   DAOSCV010000108 DAOSCV010000000
VERSION     DAOSCV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7618_length_6476_cov_3.261953"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLY56_04020"
     CDS             complement(<1..366)
                     /locus_tag="PLY56_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ20676.1"
                     /translation="MARTILHVDMDAFYASIEQRDHPELLGKPVIVGGSPEERGVVSA
                     ASYEARRYGVRSAMPMAQARRLCPEGVFLPVRMARYRQVSSQVMAILHCFTPLVEKLS
                     VDEAFLDVTGSEALFGSGVE"
     gene            complement(625..2847)
                     /locus_tag="PLY56_04025"
     CDS             complement(625..2847)
                     /locus_tag="PLY56_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20677.1"
                     /translation="MTTRRLALPALLLWILAVLPRPAAARQNIMEYYDPDNKDIPVLI
                     FSSGEPKEFGTRFLSRPGVEYLPLSQMAARKEQLDKLPLVFLIAREHVMELPQELKEY
                     LPMIADLKPTELFIYGDTPEDRCEYKAPGERRGRKYERTRLVIVAPTEEWLRRAIDMA
                     KTLPDLPAREAKRKSPRFPNPLAAELPILCTVAGKGKPAISRFAQTAYRASQSQALGN
                     PAALLREIALGHRPLIQFDADPGSWERHKPWLPHAEEAYLLLYEELDDLPVSVRSRLP
                     REVFSLAPNQTLAVRKPRADAPPVSCLIAPTPYLLNSLIERLGSSTIPSTPEVVDVPD
                     LRHVETITIAGTSNLTDVQTNLKAQLEERLMGVVSNARLFKGVRAGSDQLNTVLDTIR
                     VEMAGVTKKDLDDVRVIANTDALLLAAITRAGGKTVYQSQRQQLTPNEPAFSESEPKK
                     PDPNDRTRPFGPHKYTLDYGGKRENDPRYKDDLAKWEKEHAEWEKRKRDYERKLAAKR
                     FEWKWQVTQRQEATVEITIGVFDLKAGAVVWASQPLVGQAELSGIAKERVIVVTGQGN
                     APSDPEETPLTRDTASGELFSQALRKAVDAVPAVLRDHLLLPGDLPSAVISAGGAAED
                     SLGERAASQPGEQEIPIEGAIGVLLDASPERVVVRIDRPDPAIKVGSILLAIADLEIR
                     RDLDGTFLDVIERATVPIRVTAVYQRTCDGVPVNPAQAGRLKVRQPIRLPREGEALPE
                     "
     gene            3162..3695
                     /locus_tag="PLY56_04030"
     CDS             3162..3695
                     /locus_tag="PLY56_04030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ20678.1"
                     /translation="MAIEELCLESKVVYEGKLLKVRADRVHLPGGSEGVREVVQHPGA
                     VAMVPITEDGLVVLVEQWRYAAGGPLLEIPAGTLEPGEEPAACAARELAEEIGCRAGR
                     LELLTSFFVAPGYSSERIHLFLARDLEPAEAEADADEAIEEVLVPLADAVAMVKDGRI
                     EDAKSCLGILLAAERCA"
     gene            3789..5057
                     /gene="serS"
                     /locus_tag="PLY56_04035"
     CDS             3789..5057
                     /gene="serS"
                     /locus_tag="PLY56_04035"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ20679.1"
                     /translation="MLDIRWLCKHPDEAAERLASRGRPVPLQEVLELDREHRALLTEL
                     EARRAAHNRLSQEINRRVKAGAPAGDLPHRAREVAETLAALEPRLREVEARLRDLLLA
                     LPNLPHPDVPAGTDQTANRIVRASGEQVRLPWARPHWEIAQALDLLDPPRATAIAGSR
                     FPLLKGEGARLQRALSHFMLDLAIGRGFTEIAPPLLATGEALLATGHLPKFEDGLFQT
                     REGLSLIPTAEVPLVNLHRDEVLEGETLPRRYAALTPCFRKDAGAPGRETRGLIRVHQ
                     FEKVELVSFTRPEDSGAELERLVVAAEAVASALRLPYRVVELCAGELGFTARRTYDLE
                     VWCPGLGRYLEVSSCSDCGDFQARRCATRYRDGSGRPRFVHTLNASALAVGRTLAALL
                     ENGLQEDGSVVLPPALRPYLGGKDRIARER"
     gene            5054..>6476
                     /locus_tag="PLY56_04040"
     CDS             5054..>6476
                     /locus_tag="PLY56_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20680.1"
                     /translation="MTTSERLRWLRACGVGLLMALSLALRLPTLGGINFTPDAAEYAG
                     MARRLAEGHGLTTALKWHFFDHGPVVRPAVVERPILYPLLGGLVLRLFPEAEPLRALQ
                     ATNAALAAVNTALALAAFHTVVPAPVAWLAAALFVLLPPVTETATLALSEQLFLSLWL
                     LALLLLPGVTRPRRAAAFGLVAGLATLARPNGILLFLALPWLLSPVRRHRNARRATLA
                     ALAASGAVLLPYFLYAWTARAPEMRPAALVNYAVLHIHQATWHGFGQTIPSPPAFIAG
                     HLGAVAAAIHRKVEANLAALWVSLFPLPLLLLLRVHARPADPTRALCRAWLTLAVASF
                     AFYSLAWVAAGALRYLLPGTILLLPLLLEHGFRVARSSPAARGLFCLTLVGAVSLFGA
                     HWQEHQKACRYRWNRAAGLPYAQAAKWIEELAGPEDVVASNNPWEVHYQTCRPAVACP
                     FFRDRADLTRLRRQFGVRWVLLFA"
BASE COUNT          963 a         2174 c         2185 g         1154 t
ORIGIN      
        1 ctctactccg gatccgaaaa gcgcctcgct gccggtgacg tcgagaaacg cctcgtccac
       61 cgagagcttc tccaccagcg gcgtgaagca gtgaaggatc gccatcacct gggacgagac
      121 ctggcgatac cgggccattc gcacgggaag gaagacaccc tcggggcaga gccggcgggc
      181 ctgcgccatc ggcatcgcgg agcgaactcc gtagcgccgg gcttcgtagg aggcggccga
      241 taccacgcca cgctcctcgg gactgccacc gacgatcacc ggtttcccga gcaactcggg
      301 gtggtcgcgc tgctcgatgg atgcgtagaa ggcatccatg tccacatgca agatcgtccg
      361 tgccatcgcg tgcgcccctg ccgggagggc ctgcccggct acagcgatta tagcacgcgg
      421 ccgtggacca ggcaagcgag caggcgcgat agacgagggt ccggccctca cccgcgcgta
      481 gagcggcagc tccgtgctgc ttctcagcaa agccctgccg gaagtgtagt ccaactgagc
      541 ccggcagggc ttcgctcgca aggagcgcgg agctttaggt ccgcgcgata cgcgacgggc
      601 tggatagtct ccggggatgc cagcctactc aggaagcgcc tcgccctcgc gcggcaggcg
      661 tatgggctgg cgcaccttca agcgtccggc ctgggccgga ttcaccggaa caccgtcgca
      721 ggtgcgctgg tagacggcgg tgacgcggat cggcaccgtg gctcgctcga tcacatcgag
      781 gaaggtgcca tccagatcgc ggcggatctc caggtcggcg atggccagga ggatcgaccc
      841 caccttgatc gccgggtcag gcctgtcgat cctcaccacc acgcgctctg ggctcgcgtc
      901 gaggagcacg ccgatagctc cttcaatcgg gatctcctgc tcgccgggct ggctcgcggc
      961 cctctcgcct agagaatcct cagcggcccc gcccgcggag ataaccgcgc tggggagatc
     1021 tcctggaagg aggaggtggt cgcgcagcac cgcggggacc gcgtccaccg ccttgcgaag
     1081 cgcctggctg aacagctcgc cagatgccgt gtccctcgtg agcggggtct cctccggatc
     1141 tgagggcgcg ttcccctggc cggtgacaac gatgacgcgc tccttagcta tgccgctcaa
     1201 ctcggcctgg cccacgagcg gctgtgatgc ccagacgaca gcgcccgcct tcagatcaaa
     1261 gacgccgatg gtgatttcga cggtggcttc ctggcgctgc gtcacctgcc acttccactc
     1321 aaaccgcttc gccgcgagtt ttcgctcgta atcgcgcttg cgcttctccc actcggcgtg
     1381 ctccttctcc cacttcgcca ggtcatcctt gtacctgggg tcattctccc gcttgccgcc
     1441 atagtcaaga gtgtacttgt gcgggccgaa ggggcgcgtg cggtcattcg ggtcgggttt
     1501 cttcggttcg gactcgctga aggcgggctc gttcggagtg agctgctggc gctgcgactg
     1561 gtagacagtc ttcccgcccg ctcgcgtgat cgcggcgagg agaagcgcgt cggtgttggc
     1621 gatcacgcgg acgtcgtcca ggtccttctt cgtcaccccg gccatctcga cacggatcgt
     1681 gtcgagtacg gtgttgagct ggtcgcttcc cgcgcgcacg cctttgaaga ggcgcgcgtt
     1741 gctcaccaca cccatcagcc gctcctccag ctgggctttc aggttggttt gcacatccgt
     1801 caggttgctc gtgccggcaa tcgtgatcgt ctcgacatgc cggaggtcgg gcacgtcaac
     1861 cacctcggga gtagacggga tggtggatga gcccaggcgc tcgatgaggc tgttgaggag
     1921 gtagggggtc ggggcgatca gacacgagac gggtggcgca tccgcgcgag gcttgcggac
     1981 ggcgagcgtc tggttcggag ccaggctgaa gacctcgcga ggaagccgcg agcgtacaga
     2041 caccgggaga tcatcgagct cctcatagag caggaggtag gcttcctccg catggggaag
     2101 ccacggcttg tgtcgctccc agctgcccgg atccgcatca aattggatga gcggtcggtg
     2161 gccgagggcg atctcccgca ggagggcggc agggttcccc aaggcctgag attgcgatgc
     2221 acgataggcc gtctgggcga agcgtgagat cgctggcttg cccttgccgg ccaccgtgca
     2281 gaggatcggg agttccgcgg ccaacgggtt ggggaaccgg gggctcttcc gcttggcttc
     2341 ccgcgcggga agatcgggaa gcgttttcgc catgtcgatc gcgcggcgta gccactcctc
     2401 tgtcggagcg acgatcacca ggcgcgtgcg ttcgtacttc cgcccgcggc gctccccagg
     2461 agccttgtac tcgcagcggt cttccggtgt atcgccgtag atgaagagct cggtgggctt
     2521 caggtcggcg atcatcggca ggtattcctt cagctcctgc ggcagctcca tcacgtgctc
     2581 gcgggcgatc aggaaaacga gcggtagctt atcgagttgc tccttgcgcg ccgccatctg
     2641 gctgagcgga agatactcca cgccaggccg cgacaggaag cgggtgccaa actcctttgg
     2701 ttccccggat gagaagataa ggacagggat atccttatta tccgggtcat agtattccat
     2761 gatattctgg cgcgctgcgg ccggtctggg aagaacggct agaatccaca gcaagagagc
     2821 aggcagcgcc agcctacgcg ttgtcatcgg tctacgactc ctcctggaag gcgatgcggg
     2881 gatcgctatc gatcgccagg cgcggaggat agaaagggtt ccttcacgcg caatgggcca
     2941 gcggcgtcac tgcttggcgg cgcatcggca ctcgccgggc agcgggagca acgcttccct
     3001 ctcacccgag ccagagcata gcacatcttc cctgggggtg tcaacactgg catcggcgcc
     3061 accctggcaa gcggcgaggt tgcacgtggt tgccttcagg ccccccgatt cggtatgata
     3121 gggcggcgcg ccccgcggag cgcgcggaga ggagtgggcc cttggcaatc gaggagttgt
     3181 gcctggaatc gaaggtcgtc tatgagggca agctgctaaa ggtacgcgcg gaccgggttc
     3241 atctgccggg cggcagcgag ggagtccgcg aggtggtgca gcaccccggc gccgtggcga
     3301 tggtgcctat cacggaggat ggcctagtgg tgctggtgga acagtggcgc tacgcggcag
     3361 gcggccctct gttggagatc cctgccggca cgctggaacc gggcgaggag ccggctgcct
     3421 gtgccgcgcg cgagttggcg gaggagatcg gttgccgggc cggccgcctg gagctgctca
     3481 cttctttctt cgttgcgccc ggctactcgt ccgagcggat tcacctcttc ctggcgagag
     3541 acctggagcc cgccgaggct gaggcggatg ccgacgaggc gattgaagag gtcctggtcc
     3601 ccctcgcgga tgccgtcgcg atggttaagg atgggcgtat cgaggatgcc aagagctgct
     3661 tggggatcct gctcgcggcg gagcggtgcg cgtagcgcac tccgggtggt gactggttcc
     3721 accgtgcctt cagaccggat ggacagactc gcggtcgtcg gcgacaacgc catgcgatag
     3781 gagagggcat gctcgatatt cggtggctat gtaaacaccc ggatgaggca gccgagcgcc
     3841 tcgcatcacg cgggcggcct gtgccgctgc aagaggtgct tgagctcgac cgcgagcacc
     3901 gggccctcct gactgaactc gaggcgcggc gcgcggcgca taaccgcctc tcgcaggaga
     3961 tcaaccgccg cgtcaaggca ggcgcgcccg ctggcgacct gccccatcgc gcccgcgagg
     4021 tggcggagac cctcgcggcg ctggagccgc gactgcgcga ggtcgaggcc cggctgcggg
     4081 acctgctcct ggcgctcccc aacctccccc accctgatgt tccggccggc acggaccaaa
     4141 ccgccaatcg catcgtgcgc gcgtccggag agcaggttcg actgccctgg gcgcggcctc
     4201 actgggagat cgcccaggcg ctcgatctcc tggatcctcc ccgggccacg gcgattgccg
     4261 gatcgcgctt ccccctgctg aaaggcgaag gcgcgcggtt acagcgcgca ctctcgcact
     4321 tcatgctcga tctggctatc gggcgcggct tcaccgaaat cgccccgccg ctgctcgcca
     4381 cgggcgaggc gctcctcgcc acagggcacc tgccgaagtt tgaggatggt ctcttccaaa
     4441 cgcgcgaggg cctctccctg atccccaccg cggaggtgcc cctggtgaac ctccatcggg
     4501 acgaagtgct ggagggggag acgctccccc ggcgatatgc cgcgctcacg ccctgttttc
     4561 ggaaggatgc aggcgcgccc ggaagggaga cacggggtct gatccgggtc caccagttcg
     4621 agaaggtgga gctggttagc ttcacgcgtc ccgaggattc cggcgcggag ctggagcgac
     4681 tggtcgtcgc ggccgaagcg gtcgcgtccg cgctccggct cccctatcgc gtcgtggagc
     4741 tctgcgcggg agagctcggc ttcacggcgc gccgcacata cgatctggag gtatggtgcc
     4801 cgggcctggg ccgatacctg gaagtgagct cgtgcagcga ctgcggcgat ttccaggccc
     4861 ggcgatgcgc cacccgctac cgcgatggaa gtgggcgccc tcggttcgtc cacacgctga
     4921 acgcctcggc gctggcggta gggcgcacgc tcgcggcgct cctggaaaat ggcttgcaag
     4981 aggatggctc ggtggtgttg ccgccggcgc tgcggcccta cctcggcgga aaggaccgga
     5041 tcgcgcggga gagatgacga cttccgaacg ccttcgctgg ctgcgcgcgt gcggagtggg
     5101 actgttgatg gcgctctccc tggcgctgcg tctcccgacg ctgggcggga tcaacttcac
     5161 cccggacgct gccgagtatg cgggcatggc gcgccgtctg gcagagggtc acggcctcac
     5221 aacggcgctc aagtggcact ttttcgatca cggccccgtg gtacgcccgg cggttgtcga
     5281 gcgccccatc ctctatcccc tcctcggggg cctggtgctc cgcctctttc ccgaggccga
     5341 gccgctgcgc gccttgcagg cgacaaacgc cgcgcttgcc gcggtcaaca ccgctctggc
     5401 gctcgccgca ttccataccg tggtgccggc gccggtcgcc tggctggcgg cggcgctttt
     5461 cgtccttctc ccgccggtca ccgaaacggc cacccttgcg ctcagcgagc agctcttcct
     5521 ctcgctctgg ctactcgcgc tgctcctgct gccaggggtc actcgcccgc gacgcgcggc
     5581 ggcgttcggc ctcgtcgctg gcctcgccac ccttgcccgg cccaatggca tcctcctctt
     5641 cctcgcgctg ccgtggctgc tatcgccggt caggcgccat cgcaacgcgc gccgtgccac
     5701 cctcgcggcg ttggctgcgt ccggcgccgt cctcctgccc tacttcctct acgcctggac
     5761 agcgcgcgct cccgagatgc gccccgcggc gctggtgaac tacgccgtgc tgcatatcca
     5821 ccaggcgacc tggcatggtt tcgggcagac gatcccatct cctccagcct tcatcgccgg
     5881 gcatcttggc gccgtggccg cagcgatcca ccgcaaggtg gaggcgaacc tcgctgcatt
     5941 gtgggtatcg ctcttcccgc tcccgctgct gttgctgctg cgcgtacatg cccggcccgc
     6001 ggatcccacg cgcgcgctat gccgcgcctg gctcaccctc gcagtcgcca gcttcgcgtt
     6061 ctactccctc gcctgggttg cggcgggagc gttacgctac ctgctccccg gcaccatcct
     6121 tcttcttccc ttgcttctgg agcatggctt ccgtgtggcg cgctcctcgc cggcagcgag
     6181 ggggctgttc tgcctcacct tggtcggcgc cgtctccctc ttcggcgcgc attggcagga
     6241 gcatcagaag gcgtgccggt accgctggaa tcgcgccgcc gggctgccct atgcgcaggc
     6301 cgcgaagtgg attgaggagc ttgcgggccc ggaggatgtg gtcgccagca acaacccgtg
     6361 ggaggtgcac taccagacgt gccggccggc cgtggcgtgt cccttcttcc gagaccgcgc
     6421 ggatctcacc cggctgcgac gccagttcgg cgttcgctgg gttctcctct tcgcct
//
LOCUS       DAOSCV010000109         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7674_length_6441_cov_3.289383, whole genome shotgun sequence.
ACCESSION   DAOSCV010000109 DAOSCV010000000
VERSION     DAOSCV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7674_length_6441_cov_3.289383"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PLY56_04045"
     CDS             <1..976
                     /locus_tag="PLY56_04045"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOJ20681.1"
                     /translation="YGLGRAVAFTSDCKARWGAHWLTWPGYQRFWGQAIRWTLRRSAR
                     AHFQTHVEVEHGRGRIRVDALDEKGEFANFLEVRANVVRPDMVAQSLVLPQSGPGRYE
                     GEFDATAIGTYMVNITRSDQSGTASQTTGFAISYPPEYQDLKTNHFLLTRLAERTGGR
                     VLSDPADVFTKERRRARVPVEIWPALLLAALLLFPLDVAVRRVQMEPAEIRAWARTRA
                     PWLRSRVARRKPEAVERPETMGRLLERKSRVATAQRKTQVEGEARVVSPRVLSGGAEQ
                     ETAPPVESEPARSARQTPEPPVPEAEAHVRPTERLLRAKRRARGEDPP"
     gene            complement(1054..1227)
                     /locus_tag="PLY56_04050"
     CDS             complement(1054..1227)
                     /locus_tag="PLY56_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20682.1"
                     /translation="MEILYRSLFVLGYILLVPSLTLFWYTGGRRMERLQSRSLVVMGI
                     GMALILLSAQPYR"
     gene            1560..3533
                     /locus_tag="PLY56_04055"
     CDS             1560..3533
                     /locus_tag="PLY56_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOJ20683.1"
                     /translation="MFKLRFRQIHLDFHTSEQIPGIGSRFDPEEFAETLARARVNSVT
                     AFSKCHHGLIYHETRFEARHPHLQRNLLKEQIDACHARDIRVPIYISVGFDEFMARRH
                     PEWLEVDGNGRRTGAAPLQPGWHKLCFNSPYLDYVLEQTLEVMELFAPVDGFFFDIIG
                     QGQCHCPFCLDSMRQEGLNPEEEADRLRFAKRVVDRFKRRFTAAIRERSADCSIFYNS
                     GHIYPDWRPILDTYTHLELESLPSGGWGYLHFPLTVRYARTLGFDYLAMTGKFHKSWG
                     DFSSYKNLPALEYDCFTALAYGAKCSVGDQLHPTGQLSRATYDLIGAVYRQVEAKEPW
                     CDDVVPEVEIGLFNPEAVGNADARVDTSAAGAQRMLTEGHHQFDVIDAANDWSRYRVI
                     LLPDKIPMTEEIRGKVAEYLAGGGSLITSYESGMGPDKRGFVIPGMPAGFIEPAEFSP
                     DFVVARDPLAEALGDAEQVMYDRGILLKPAADAEVLADIWNPYFNRTWEHFCSHSHTP
                     CERPSGAPAVVQRGRIIQFAHPIFAMYQRHGVRAYKQMVLSALARLLPDPLVKTDAPS
                     TAHLTVNRQPEQRRSVLHILHYIPERRANTLDTIEDVIPLYHLRVALKREQEPARVYL
                     APSGDEVACTYRDGYAEVVVPEVRGHQMVVFED"
     gene            complement(3593..4534)
                     /locus_tag="PLY56_04060"
     CDS             complement(3593..4534)
                     /locus_tag="PLY56_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOJ20684.1"
                     /translation="MRRLVALCALLAFGSVLAGCANDDEIEAPTITRLVQEDGRFSRL
                     AIALQATGLQVALRQSGPFTLFAPTDQAFAGLPSGTFELLLQNPADLGRVLQYHVVPT
                     RITEAELRALADQNATLNTLQDARLDIRSADGALVLNPGGERAFVEGDPLVASNGLVY
                     PINMVLAPPLVGKDLVDALRFDPQFAQLVQLLEAAALDEILRQPGPFTLFAPTDQAFA
                     RLPASTLDALIADPAQLDRILRFHVVPGRFTTADLRGLSSLNTLLGPHFAITSTQMDT
                     LVIGSAFGEALLIRPDFGVANGIIHTIDNVLFPPSGP"
     gene            4831..>6441
                     /locus_tag="PLY56_04065"
     CDS             4831..>6441
                     /locus_tag="PLY56_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase"
                     /protein_id="HOJ20685.1"
                     /translation="MEEYRRKAEHFLKHETQFRLGMLPTEQSHPRTRGLAETLQREPR
                     AGIRMLQDVDQDVRASASRTFQSAEYRSLVAALIEAFRAGRRVCFSGCGATGRLSILL
                     EACWRRFWRDLGIHSHALEQTSASLADRVCSLMTGGDYALIRSVENFEDYASFGRRQV
                     AEAGLGAGDVLVAISEGGETSSVIGTIHEALARGARAFFVFNNPAEVLARHIERSRAV
                     IECPAVTILDLTSGPMAVAGSTRMQATTAELLVVGSALEEALIRLLPKLLPRDMLSTV
                     PGEWRTPGDAAARFTALLADLAHPDAVDAIAEWVTIERDLYAARGRMTYYASDCLLDI
                     FTDTTERAPTFMLPPFRKCDDPVSPPSWAFVKNPLLETPAAWREVLCREPRCLEWDQA
                     CYEELQGPPSARANPPRLGRGELLKFLIGCEEDPSRFEAPDSAAAAVLLSREIHASEF
                     SAWWSAFTAAAQRYRQRLAVIIGADAALLPQVERVVHVPCRPPQTPLGIWNRLAVKLV
                     LNTLSTATMGCLGRLTSNWMANVEATNKK"
BASE COUNT         1097 a         2092 c         2140 g         1112 t
ORIGIN      
        1 ctatggcctc ggccgcgccg tcgccttcac ctccgactgc aaggcgcgct ggggggcgca
       61 ctggctcacc tggcccggct accagcgttt ctggggacag gcaatccgtt ggacgcttcg
      121 ccggagtgcg cgcgcccact tccagaccca cgtggaggtg gagcatggcc gggggcggat
      181 ccgcgtcgat gccctggatg agaagggcga gttcgccaac ttcctcgaag tgcgcgccaa
      241 cgtcgttcgc cccgatatgg ttgcgcagtc gctcgtgctg ccgcaatccg ggccgggccg
      301 ctacgagggc gagtttgacg ccaccgcaat tggcacctac atggtgaaca tcacccgctc
      361 tgatcagagc ggcaccgctt cgcagaccac cggcttcgca atctcctatc caccagagta
      421 ccaggatctg aagacgaacc actttctgct cacccgcctg gctgagcgca ccggtggccg
      481 cgtcctctcc gaccccgcgg atgtttttac caaggaacgc agacgggcgc gagtgccggt
      541 ggagatctgg ccggcgctgt tgctggcggc gctactcctc ttccctctgg atgtggcggt
      601 gcgccgggtg cagatggagc ccgcggagat ccgcgcgtgg gcgcggactc gtgccccctg
      661 gctgcgctcc cgagtggcgc gccggaaacc agaggccgtc gagcgcccgg agacgatggg
      721 ccggctcctg gaacgcaaat ctcgtgtggc cacggcgcag cggaagacgc aggtggaggg
      781 tgaggcgaga gtggtttcgc caagggtact atcgggtggg gctgagcagg agacggcgcc
      841 cccggtagaa tccgagccgg cgcgctccgc ccggcagact ccagagccgc cggtgcccga
      901 ggccgaggca cacgtccgtc cgaccgagcg cctgctacgc gccaagcgac gcgcgcgtgg
      961 cgaggatccc ccgtagcctc ccgggcgtcc gagatcacgg gatagaaccg gaaccgcgca
     1021 tcggatagca gggcgcggtg gccggtggga gggctatcgg tagggctgcg cggagagaag
     1081 gataagcgcc ataccgatcc ccatcacgac gaggctgcgg ctctgcaacc gttccatacg
     1141 ccgcccaccc gtgtaccaga agagtgtcag cgaggggacc aacaggatgt agccaaggac
     1201 gaacagactc cggtagagta tttccatcgt atctgagtgt gttccaggtt cacagggtcc
     1261 cccaaaggca ggggagaagg gctttcaggc ttctgaactc ctgttcggca tcttggccgc
     1321 cgattcctcc cagtggatcg tatcaggaag caagcgcgtg cgagcggagg gccagccctc
     1381 tgagggcagc ggcatcttga gccgacgcgt gccggggcga ccggacgcca ggaggggccc
     1441 agtccgtcgc cgaacgcttc ttccggcgcc ggcctgcgcc ggcgcctatc gggtggggcg
     1501 cgatgccatg tgctgccaga gcgcccggca tgtgctgtga tacgcgggag atggatgcga
     1561 tgttcaagct acgcttccga cagatacacc tggatttcca cacctcggag cagatccccg
     1621 ggatcggctc gcggtttgat ccggaggagt ttgccgaaac gctggccaga gcccgggtga
     1681 actcggtgac ggccttctcg aaatgccacc acgggctgat ctaccatgag acgcgcttcg
     1741 aggcgcgcca cccccacttg cagcggaatc tgctcaagga gcagatcgac gcctgccacg
     1801 cgcgcgacat ccgcgtgccg atctacatca gcgtcggctt cgacgagttc atggctcgcc
     1861 ggcatccgga gtggctcgag gtggacggca acgggcggcg caccggagcc gcgcctttgc
     1921 agcccggctg gcataagctg tgcttcaact cgccttacct ggattacgtg ctagagcaga
     1981 ccctggaagt gatggagctc tttgcaccgg tggatgggtt cttcttcgat atcattggtc
     2041 agggccaatg ccattgcccc ttctgcttgg acagcatgag gcaagagggc ctcaaccccg
     2101 aggaggaggc agaccgcctg cgttttgcca agcgcgtggt cgatcgcttt aagcggcgtt
     2161 tcacggcggc gattcgtgag cgcagcgctg attgctccat cttctataat tcggggcata
     2221 tctatccaga ttggcgtccg atcctggaca cctacacgca cctggagctt gaatcactgc
     2281 cgagtggggg gtggggctac ctccacttcc ccctcaccgt gcgctacgcg cgcacccttg
     2341 gcttcgatta cctggcgatg accggcaagt tccataagtc gtggggcgac ttctcctcct
     2401 ataagaacct gccggcgctg gagtatgact gcttcaccgc cctcgcctat ggcgccaaat
     2461 gctcggtcgg cgaccagctc catcccacgg ggcagctctc gcgggctacc tacgacctca
     2521 tcggcgcggt ctatcgccag gtggaggcga aggagccctg gtgcgacgac gtcgtccccg
     2581 aggtggagat cggcctcttc aatccggagg cggttggcaa cgccgatgca cgcgtcgata
     2641 cttccgcggc gggtgcccag cgcatgctga ccgagggcca ccatcagttc gatgtgatcg
     2701 atgccgcgaa cgactggtcg cgttaccgcg tgatcctctt gccggataag atcccgatga
     2761 ccgaggagat ccgcgggaag gtggccgagt acctggccgg cggtggcagc ctgatcacct
     2821 cctacgaatc ggggatgggc ccggacaagc gcggcttcgt gatccccggg atgccagcgg
     2881 gtttcattga gcccgcggag ttctcgcccg atttcgtcgt tgcccgcgat ccgctcgccg
     2941 aggccctcgg ggacgcggag caggtgatgt acgaccgcgg catccttctc aagcccgcgg
     3001 cggatgccga ggttctggcc gatatctgga acccctactt caatcgcacc tgggagcact
     3061 tctgctcgca ctcacacacc ccgtgcgagc gtcccagcgg agcgccggcg gtggtgcaga
     3121 ggggtcgcat catccagttt gcgcatccga tcttcgcgat gtatcagcgc cacggggtgc
     3181 gcgcctataa gcagatggtg ctcagcgcgc ttgcccgcct gctgccggac ccgctggtga
     3241 agacggatgc gccttccacc gcgcacctga ccgtcaaccg gcagccggag cagcgccgga
     3301 gcgtgctcca tattctccac tacattcccg agcgccgggc caacactctc gataccatcg
     3361 aggatgtcat tccgctctat catcttcgcg ttgcgctcaa gagggagcaa gagccagccc
     3421 gcgtctatct ggcgccttca ggtgacgagg tggcgtgtac ctatcgcgat ggctacgcgg
     3481 aggtggtggt gccggaagtg cgcggccatc agatggtcgt cttcgaggac tgatatcggc
     3541 ttgcaccagg gggagatgcc gaagcggtca tctccccctg cgaaggtggc ttctacggcc
     3601 cagacggcgg gaacagcacg ttatcgatgg tgtggatgat cccgttggct acgccgaagt
     3661 ccgggcgaat cagcagggct tccccgaacg ccgagccgat caccagagtg tccatttggg
     3721 tgctggtaat ggcaaaatgc ggccccagca gcgtgttcag gctgctcaaa ccgcgcaggt
     3781 cggcggtggt gaaccggcct ggcaccacgt ggaagcgcag gatacggtcg agctgagcgg
     3841 gatcggcgat cagggcatcg agtgtgctgg caggaagccg ggcgaatgcc tgatccgtgg
     3901 gcgcgaagag ggtgaacggc ccaggctggc gcaggatttc atccagcgcg gccgcttcga
     3961 gcagttgcac caattgggcg aactgcgggt cgaagcgtag cgcatccacc aggtctttgc
     4021 cgaccagcgg cggcgccagc accatattga tcgggtagac caacccgttg cttgccacaa
     4081 gtgggtcgcc ttccacgaac gcgcgctcac cgcccgggtt gaggaccaac gccccgtcgg
     4141 cagatcggat atctaaacgg gcatcctgca gcgtgttcag cgtcgcgttc tgatctgcca
     4201 gggcgcgcag ctccgcctcc gtgatgcggg tgggcacgac gtggtattgc agcacgcggc
     4261 ccagatccgc agggttctgc agcagcagct cgaacgtacc ggacgggagc ccggcgaatg
     4321 cctggtcggt gggcgcgaaa agggtgaacg ggcccgactg ccgcagtgcc acctgcagcc
     4381 ctgttgcctg cagggcgatt gccagccggg agaagcgacc atcttcctgg acgagtcggg
     4441 tgatggttgg ggcctcgatc tcatcgtcgt tggcgcaccc ggccagcacg gagccgaacg
     4501 caaggagcgc gcacagcgca accagtcgcc tcatccttct tctcctcgga agggccaacg
     4561 agcgattccc tattccactc gctcgcctag acgaaacatc ggcgcgatgg gcgaggtggc
     4621 gggggcgccg cgtcgcgcag gagtaaacgc gtgccgcaga gcagagggcg tgtgcataaa
     4681 cagcgcgggc cttcagctcc gcgctcaggc ttgacagaga cgccgttccg gcgtatgatc
     4741 ccgcgcgaag agcggttggc cgcgcttcaa tctgtctgcc acgtaggaag caatcgccca
     4801 ccgggagaag agcacggagg atccaacacc atggaggagt accgccggaa agccgagcac
     4861 ttcctgaagc acgagacgca gttccgtctc ggtatgctgc cgaccgagca atcacacccc
     4921 cgcacccgcg gcctggccga gacgctgcaa cgcgaaccgc gcgccggcat ccggatgctg
     4981 caggacgtgg atcaagacgt aagggcgagc gcctcccgca cattccagag cgcggagtac
     5041 cggtcgcttg tggccgcgct catcgaggcg ttccgtgcgg gccggcgcgt ttgcttctcc
     5101 gggtgcggcg ctaccgggcg gctcagcatc ctgctagagg cgtgttggcg gcgcttctgg
     5161 cgcgatctgg ggatccattc ccacgcgctc gagcagacca gcgcctcgct cgcggatcgc
     5221 gtctgcagcc tgatgacggg cggtgattat gctctgatcc gctcggtgga gaactttgag
     5281 gactatgcct ccttcgggcg caggcaggtg gcggaggccg gtctgggggc cggagacgtc
     5341 ctcgtcgcca ttagcgaggg cggcgagact tcctcggtga tcggcacgat tcacgaggcg
     5401 ctggcccgcg gcgcacgcgc cttcttcgtc ttcaacaacc ccgccgaggt tttggcccgg
     5461 cacatcgaac gctcgcgcgc ggtgatcgag tgcccggccg tcaccatact agacctgacc
     5521 tccgggccga tggcggtcgc cggatccacc cgcatgcagg ccacgaccgc cgagctgctg
     5581 gtcgtgggct ccgcgctgga ggaggcgctc atccggctgc ttcccaagct tcttccccgt
     5641 gacatgctct ccacggtgcc tggtgagtgg cgaaccccag gggatgccgc cgctcggttt
     5701 acggcgctgc tcgccgatct cgctcacccg gacgcggtgg atgccatcgc cgagtgggtg
     5761 acgatcgagc gcgacctgta tgccgcccgg ggacgcatga cctactacgc ctccgattgc
     5821 ctcctggata tcttcaccga taccacggag cgcgcgccga ctttcatgct tccccctttc
     5881 cgcaaatgcg acgatccggt ctcaccaccg tcgtgggcgt tcgtcaagaa cccgctcctg
     5941 gagacgccag ccgcctggcg ggaagtcctc tgtcgggagc cacggtgcct ggagtgggac
     6001 caggcgtgct atgaggagct ccagggcccg ccgagcgccc gggcgaatcc gccccgcctg
     6061 ggccggggcg agctgttgaa gtttctcatc ggctgcgagg aggacccctc tcgctttgaa
     6121 gcgcccgact ccgcggcggc ggctgtgctc ttgagccgcg agattcatgc ttcggagttc
     6181 agcgcgtggt ggagcgcctt caccgcggca gcgcagcggt atcgccagcg cctggccgtg
     6241 atcatcggcg cggacgccgc gttgctcccc caggtagagc gcgtggtgca cgtcccctgc
     6301 cggcctcccc aaaccccctt gggaatctgg aaccgcctgg cggtaaaact ggtgctcaac
     6361 accctttcca ccgcgacgat ggggtgcctc gggcggctga cgagcaactg gatggcgaac
     6421 gttgaagcca caaacaagaa g
//
LOCUS       DAOSCV010000110         6435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7689_length_6435_cov_3.306897, whole genome shotgun sequence.
ACCESSION   DAOSCV010000110 DAOSCV010000000
VERSION     DAOSCV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7689_length_6435_cov_3.306897"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="PLY56_04070"
     CDS             complement(<1..1504)
                     /locus_tag="PLY56_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20686.1"
                     /translation="MRLLAYAIAWICAASFCLAAPQPGNRIQNGGITEVEGDKPAQWG
                     TYVAGGDAPLFTVEEAGARGKVLRLEATHEKTRGFWVSPGFPVTPGERLTVSAAMRSA
                     LTKGSAMLSIGFRGGDGLAFGIRDLAHLTGTAEWETRAQSVEVPAGAKSGYLTLGVGP
                     GTTGTIWGDDFAVVACPLRISLKESGLKAAEGRVLGFSLEATGDLPEKIRVELRLDGA
                     GKLEERVLETAGKRRFDIAFRTDRVDAIQIAAHVFSPEGLLLAADTLEAPPVMEASLG
                     KASYRNTLFVTKESPRVLEASVTLHAEPAALRGLALRAALVAPGNATPLATHETRVTG
                     DRVPFRLELPRVPNGRYQLQLALISAGKPVAQAELPFAIREDGPNTVRIDEHLNLVVG
                     GKPFFPHGVYGGAPPEQLPNIKAGGFNTLFAYDSNPESLRKLLDKAQEVGLYVMISAA
                     LPFAGKLPAEREKLREVVLGLKDHPALLGWYLYDEPDGQGIAPSIIRDLHE"
     gene            complement(1566..2387)
                     /locus_tag="PLY56_04075"
     CDS             complement(1566..2387)
                     /locus_tag="PLY56_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ20687.1"
                     /translation="MEIKIGCNTLYSAGRLSNEDAFTLPHQLRALEIIADAGFDAVEY
                     SHASVLTLEELAQVAERTRALGLIPWSVHAWTPLAADDAQVEPTLAAFQSAAEIARAL
                     GTRVVVVHSAGGMDPARLEERKRANQTTLRALAEAVGPETVVAVENMRSLADWEFLLN
                     MVATCGAANVGVNIDTGHANLGDLGVVRAIEMAGAAIQTTHLQDNFGERDDHLPPGLG
                     QIDFVAALAAFRKAGYTGVYMVEISDCPPGREPDAVADTQAAARNLRSFLAQAGF"
     gene            2521..3219
                     /locus_tag="PLY56_04080"
     CDS             2521..3219
                     /locus_tag="PLY56_04080"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20688.1"
                     /translation="MAMRRVWLWLAMACAILVAGQGMIRAAAPKTRMAVITRLRRGQW
                     PVIDGRFGDPAWQGVRPLQAFVPLGGDGAKPKAETVAWVTYDDARLYLFIRCREPRPE
                     EMRVAGEGRDDDVGEGDSLHLFIATADLPDPSFYFALNPKNVRWDAFCNGETIEPAID
                     CEWESAVVAGKKEWCAEIAIPWSALRREPPLPGTKIRVNLGRRRIPDDERTTWSPAEK
                     QFHEPERFGTWVLQ"
     gene            complement(3226..4005)
                     /locus_tag="PLY56_04085"
     CDS             complement(3226..4005)
                     /locus_tag="PLY56_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ20689.1"
                     /translation="MIKIYIHTDLEGVSGIDSFEMMQRTSETYRRCCELLMGDLNAAI
                     DGAFAGGADHVTVLDSHGGGNNFIPEMLDPRAENDTRPNKKWWGILDESYQGTFFIGA
                     HAMSGTMNAFLDHTQSSIHWHDYSINGRRMGELAQWAIVASNWNVPMLMVSGDEAACV
                     EARQFFHPVETAVVKRAIGRNRAELVDLEEARAQIREAARRAVSLIGQARPFSPLKPM
                     EILLEYNRADYCDAAATRPGVERLDARTIRKVTSDPLDILP"
     gene            complement(4081..5154)
                     /locus_tag="PLY56_04090"
     CDS             complement(4081..5154)
                     /locus_tag="PLY56_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20690.1"
                     /translation="MRFGVIGLGSAGRSHLDVLSHMPEVEVAGIADLNPEYVKTLGEK
                     YGVPLATTQPEELITNPSIDVIAVVAADRAHYPLVMACAAARKHVLCEKPIATEVAHA
                     REMVAAMRESGRLFCISLNNRGNPVNRRIKDVVDAGIIGHARMVRLIGLMAAPDNRVV
                     RERLGEKAAYSRLVNICHEGKNAMFDCGVHSFDYARFLTGSDFRRIEAMGYSMRGFEY
                     PDHGVALCEHENGILTVVDKGFDYAYEAQTRKEYVRYEVIGDRGSLAWDLDNQRLRVF
                     AHDQTLDEPLPYASHDADREVIYRGFIESVKQGTLLPWLASGEDGLKAVEAAQAAIDS
                     MIAKGIITRDVGPGPNWFDGRSW"
     gene            5280..6275
                     /locus_tag="PLY56_04095"
     CDS             5280..6275
                     /locus_tag="PLY56_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOJ20691.1"
                     /translation="MEKREPLIARALLEIGGAFQTSRPLLTAVELDLFTRLGDARLTA
                     SELARAMGVDERALSYLLDALAAQRLLEKVSGGYQNTAEGRTLLVRGAPGSILAVLEH
                     YANLWARWATLTEVVRVGHPVARREEGGAGSLESFLGAMHALAQESAPQVAEAIGLKG
                     VRRMLDVGGGAASYSIAFAQAEPGLTAVVFDLPDVVPIARRNIEAAGLQERITTQAGN
                     YHEDAFETGFDLVLLSAIVHSNSQQENAALVRKCFDALAPGGRLVIRDFIMSPDRTEP
                     APGALFAINMLVNTPGGGTFTEAEMRSWLDAAGFTDTRRVDLPGMNALMIGTRKA"
BASE COUNT         1006 a         2161 c         2189 g         1079 t
ORIGIN      
        1 cctcatgcag gtcgcgaatg atcgagggcg cgatcccctg gccgtccggc tcgtcataca
       61 ggtaccaccc caggagtgcg ggatggtctt tcaggccaag caccacttcg cgcagcttct
      121 cacgctccgc gggcagcttc ccggcgaagg gaagcgccgc ggagatcatc acgtacagcc
      181 ccacctcctg ggccttgtcg aggagcttgc gcaggctctc ggggttgctg tcgtaggcaa
      241 agagggtgtt gaacccaccc gccttgatat tgggaagctg ctctggcggc gctccgccat
      301 acaccccgtg cgggaagaac ggcttgcccc cgacgaccag gttgaggtgc tcgtcgatgc
      361 gcaccgtgtt cgggccgtcc tcgcggatgg cgaaaggcag ctcggcctgc gcgaccggct
      421 tgcccgccga aatgagcgcc agttgcagct ggtagcgccc gttgggcaca cgcggcagct
      481 ccagccggaa cggaacgcgg tcgccggtga cccgggtctc gtgtgtggcc agcggggtcg
      541 cgttccccgg ggccacaagc gcggcgcgca gcgccagccc cctcagcgcc gcgggctccg
      601 catggagcgt gacgctcgct tccaggacgc gcgggctctc cttcgtgacg aaaagcgtgt
      661 tgcggtagga cgctttgccg agcgaggcct ccatgaccgg cggggcttcc agagtatccg
      721 ctgcgagcag gagcccctcg ggcgagaaga cgtgggcggc aatctggatc gcatccaccc
      781 ggtcggttcg gaacgcgatg tcgaagcgcc gcttgccggc ggtttcgagg acgcgctcct
      841 ccagcttccc ggcgccatcg aggcgcagtt cgaccctgat cttctcgggc aggtcaccgg
      901 ttgcctccag cgagaagccg agaacccggc cctccgcggc cttcaagccg ctctccttga
      961 gcgagatccg gagcgggcag gccaccacgg cgaagtcgtc gccccagatg gtgccggtgg
     1021 tgcccggtcc cacgccaagg gtgaggtagc cgctcttcgc gccggctggc acctccacgc
     1081 tctgcgcgcg ggtttcccac tccgccgtgc ccgtcaggtg ggccaggtca cggatgccaa
     1141 aggccaggcc atcaccgccg cgaaacccga tgctcagcat cgccgagccc ttcgtcagcg
     1201 ccgagcgcat cgcggcggag acggtgagcc gctctcccgg ggtgaccgga aagccgggcg
     1261 agacccagaa gccccgcgtc ttctcgtgcg tggcttccag gcgcagcacc ttcccgcgcg
     1321 cgcccgcctc ctcgacggta aagaggggcg cgtcgccccc ggcaacgtag gtaccccatt
     1381 gcgcgggctt gtcgccctcg acttctgtga tcccaccgtt ctggatccga ttgcctggct
     1441 gcggtgccgc caggcagaac gatgccgcgc agatccatgc gatggcatac gcgagtagtc
     1501 tcatctggct ttctccactc ctcaaaggca cggaccggca cggcgcctcc ccacgtgccc
     1561 gcgcctcaga accctgcctg cgcgaggaag gagcgcaggt tgcgcgcggc cgcctgagtg
     1621 tctgcgaccg cgtccggctc tcttccgggc gggcagtcgg agatctccac catgtaaacg
     1681 cccgtgtagc ccgcctttcg gaacgcggcg agcgccgcga caaagtcgat ctgaccgagc
     1741 ccgggcggga gatggtcgtc gcgctcgccg aagttgtcct gaaggtgggt tgtctggatc
     1801 gcggcgccgg ccatttcgat cgcgcgaacg acgcccaggt cgcccaggtt cgcgtgcccc
     1861 gtgtcgatat tgacgccaac gttcgccgcg ccgcaggtcg ccaccatgtt gaggagaaac
     1921 tcccagtcgg ccagcgagcg catgttttct acggcgacca ccgtctccgg gcccaccgcc
     1981 tcggcaaggg cgcgcagtgt tgtctggttc gctcgctttc gctcctcgag ccgggccggg
     2041 tccatgccgc cggcgctgtg gaccacgacg acgcgggtgc ccagagcccg ggcgatctcc
     2101 gcggcgctct ggaacgccgc gagcgtcggc tcgacctgcg catcatccgc ggcgaggggc
     2161 gtccaggcat gcaccgacca cgggatcagc ccgagcgcgc gcgtgcgctc cgccacctgc
     2221 gccagctcct cgagcgtcaa gaccgaggcg tgagagtact ctacggcgtc gaagccggca
     2281 tcggcgatga tctccagggc gcgcagttga tgggggagag tgaatgcgtc ctcgttggag
     2341 agacgtcccg ccgaatagag cgtgttgcac ccgattttga tctccacaga atgccacctt
     2401 tccgggcagc caccgtgttg gcctcgccta tcacgctgcc cgacaagcca ggattccgcg
     2461 gctcgagggg cgaatccttt gcgtgtgaga cctcacgagg cgcgctccgg gagggggcga
     2521 atggcgatgc ggagagtgtg gctgtggctg gcgatggcgt gcgcaatcct tgtcgcgggc
     2581 caggggatga ttcgcgcggc agccccgaag acgcgcatgg ccgtcatcac gcggctccgg
     2641 cgcgggcagt ggcccgtgat cgatggccgg tttggcgatc ccgcctggca gggggttcgc
     2701 ccactccagg cgttcgtgcc cctgggcggc gatggcgcga aaccgaaggc ggagaccgtg
     2761 gcgtgggtta cctacgatga tgcccggctc tacctcttca tccggtgccg cgagccgcgc
     2821 cctgaggaga tgcgcgtcgc gggcgagggc cgggacgacg acgtgggcga gggcgatagc
     2881 ctgcaccttt tcatcgcgac ggcagacctg cccgatccct ccttctactt tgccctcaat
     2941 ccaaagaacg tccgctggga cgccttctgc aacggcgaga ccatcgagcc ggcaatcgat
     3001 tgcgagtggg agagcgccgt cgtggccggc aagaaagagt ggtgcgccga gatcgcgatc
     3061 ccgtggtcgg ctctgcgccg ggagccgccg cttccgggca cgaagattcg cgtgaacctc
     3121 gggcggcggc gtattcccga cgacgagcgc accacctggt cgcccgcgga aaagcagttt
     3181 cacgagccgg agcgcttcgg cacctgggtg ctccagtagc ccgctttacg gcaggatgtc
     3241 cagcgggtcg ctcgtcacct tgcggattgt gcgcgcgtcg aggcgctcca ccccgggccg
     3301 cgtggcggcg gcgtcgcagt agtcggcgcg gttatactcc aggaggatct ccatcggctt
     3361 taggggggag aaggggcgtg cctggccgat caggctgacg gcgcgccggg ccgcttcgcg
     3421 aatctgcgcg cgcgcttcct ccaggtccac cagctccgcg cggttgcgcc cgatggcgcg
     3481 cttgacgacc gccgtttcga cggggtggaa gaactggcgc gcctcgacgc aggcggcttc
     3541 atccccgctc accatcagca tcggaacgtt ccagttgctc gccacgatcg cccactgcgc
     3601 cagctcgccc atgcgccggc cgttgatcga atagtcgtgc cagtggatgg aggactgcgt
     3661 gtggtcgagg aaggcgttca tcgtgccgga catggcgtgg gcgccgatga agaaggtgcc
     3721 ctggtagctc tcgtctagga tcccccacca cttcttgttg gggcgcgtgt cgttctcggc
     3781 gcgcggatcg agcatctccg ggatgaagtt gttcccaccg ccgtggctat cgagcaccgt
     3841 cacgtgatcc gcgccgccgg cgaaggcgcc gtcgattgcc gcattcagat cgcccatcag
     3901 gagctcgcaa cagcggcggt aggtctcgct ggtgcgctgc atcatctcga agctatcgat
     3961 tccggagaca ccctcgaggt cggtgtggat gtagatcttg atcatgcgct tctccagaga
     4021 gggcccggtc gccaccggga ccctctgatc cggacgatgg caaaggcgtg ggggataggc
     4081 ctaccagctc cggccgtcaa accagttggg gccgggaccc acatcgcgcg tgatgattcc
     4141 cttggcgatc atgctgtcga tagcagcctg ggccgcctcc accgctttga gcccgtcctc
     4201 gccggacgcg agccacggca gcagcgtgcc ctgctttacg ctttcgatga agccgcggta
     4261 gatcacctcg cggtcggcat cgtgcgaggc gtagggcagc ggctcatcca gggtctggtc
     4321 gtgcgcgaag acgcgcagac gctggttatc caggtcccac gccaggcttc cgcggtcgcc
     4381 gatcacctcg tagcgcacat actctttccg cgtttgcgcc tcgtaggcat agtcgaagcc
     4441 cttatcgacc acggtgagga tgccgttttc atgttcgcag agggcgacgc cgtggtcggg
     4501 atactcgaac ccgcgcatgg agtagcccat cgcctcgatc cgccggaaat cgctgccagt
     4561 gaggaagcgc gcgtagtcga agctgtgaac accgcagtcg aacatggcgt tcttgccctc
     4621 atggcagatg ttgaccaagc gggaataggc ggccttctcg ccaaggcgct cgcgcaccac
     4681 gcggttatcg ggcgcggcca tcagcccgat caggcgcacc atgcgcgcgt gcccgatgat
     4741 gcccgcgtcc accacatcct tgatccgccg gttgactgga ttgccccggt tgttgagcga
     4801 gatgcagaac agccggccgg attcgcgcat cgcggccacc atctcgcgag cgtgcgccac
     4861 ctcggtggcg ataggcttct cgcagagaac gtgctttcta gcggcggcgc acgccatcac
     4921 cagcgggtag tgcgcgcggt cggccgcgac gacggcgatg acgtcgatag aggggttggt
     4981 aatcaactcc tcgggttgag tggtggcgag gggaacccca tacttctcgc ccagggtctt
     5041 cacgtattcc gggttgagat cggcgatgcc ggccacctcc acctcgggca tgtgcgagag
     5101 aacatcgaga tgactgcggc ccgcggagcc caggccgata acaccaaatc gcatcgatga
     5161 cttccttccc acggcaaaca cgcttacctg ttctggcccc acccctgcct ctcctctcgc
     5221 tgtcggcagg agaagcggga cggggcgggg aatgtggccc ctggagggag tggaacgcga
     5281 tggagaagag agagccattg atcgcccgcg cgctcctgga gatcgggggc gcgtttcaga
     5341 ccagccggcc gctcctcacg gcagtggagc tggatctttt cacccggctg ggagacgcga
     5401 ggctcaccgc gtcggagctc gcgcgggcga tgggcgtgga cgagcgcgcg ctctcctacc
     5461 tgctggacgc gctggcggcg cagcggttgt tggagaaggt ctctggcggc tatcaaaaca
     5521 ccgccgaggg ccggacgctc ctggtgcgag gcgcgcccgg gagcatcctg gcggtgctcg
     5581 agcactacgc gaacctgtgg gcgcgctggg ccacgctcac cgaggtggtg cgcgtcgggc
     5641 acccggttgc ccgccgggaa gagggtggtg ccggctctct cgagagtttc ctgggcgcca
     5701 tgcacgcgct ggcgcaggaa tcggccccgc aggtggcgga ggcgattggc ctcaaaggcg
     5761 tgcgccggat gctcgatgtc ggcggtgggg cggcaagcta ctccatcgcc ttcgcgcagg
     5821 ccgagccggg gctcaccgcc gtcgtcttcg acctgcctga cgtcgttccg attgcgcgcc
     5881 ggaacatcga ggccgcgggc cttcaggagc gaatcaccac tcaggccggc aactaccacg
     5941 aggatgcctt cgagaccggc ttcgacctgg tgctcctctc cgccatcgtt cacagcaatt
     6001 cccagcagga gaacgcggcg ctcgtgcgca agtgcttcga tgccctggct cccggcgggc
     6061 ggctggtcat tcgcgacttc atcatgtcgc ccgaccgcac cgagccggcc ccgggggcgc
     6121 tcttcgcgat caacatgctc gtcaacacgc cggggggcgg caccttcacc gaagcggaga
     6181 tgcgctcctg gctcgatgcc gccggtttca ccgacacgcg ccgggtcgat ctccccggga
     6241 tgaacgcgct gatgatcggc acgcgcaagg cgtaggtgcc tccgccggga cagtggctac
     6301 gcggcgatct ccaggcggac gcgaacacgg cgtgggaaaa cgtccacgta caggaagccg
     6361 gaaggcacgc cgatgctctc gcgcgacgtg ctgagcggcg gcgcggcgta gaaggggatt
     6421 ccctgccacc ggttc
//
LOCUS       DAOSCV010000111         6417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7714_length_6417_cov_3.845803, whole genome shotgun sequence.
ACCESSION   DAOSCV010000111 DAOSCV010000000
VERSION     DAOSCV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7714_length_6417_cov_3.845803"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLY56_04100"
     CDS             complement(<1..428)
                     /locus_tag="PLY56_04100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ20692.1"
                     /translation="MAPNTLKRYQIIREIARSNDIVYEAHDPQTGRRIAIKELAIPPG
                     LVGQARRERIERFRREGRAAGALSPHPHIVTIFDVDNEGDRYFIAMEYLEGQPLRDIL
                     QMEGPLPVERAIRVALQVCDALQHAHARGVIHRDIKPDNI"
     gene            complement(443..1429)
                     /locus_tag="PLY56_04105"
     CDS             complement(443..1429)
                     /locus_tag="PLY56_04105"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOJ20693.1"
                     /translation="MGSAPLESRHGGEENEITAPLELQSLREGWAKLEARAPEWHAEV
                     TFAAKTDLGRVRENNEDKFDFLEPEEPAVLAARGRFYAVCDGMGGHAAGQIASELALK
                     SIIKKYYANTTDDLETALAAAVQYANALILDTAATIPGRSGMGTTFTGAILHEEYLIA
                     AQVGDSRLYLIRDGEIRQITQDHSWVAEQIRLGVLDPESAARSPFRNVITRSLGAQES
                     VEVDLFREKIRPGDVILLCSDGLTGHLDDQEIALLARRSAPSVACMELIDAANDRGGR
                     DNITVLIIRVDAVKRFDPAAEPADPGEEPAPEAPPSPPTEGEKRRRFPLPWR"
     gene            complement(1654..2451)
                     /locus_tag="PLY56_04110"
     CDS             complement(1654..2451)
                     /locus_tag="PLY56_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ20694.1"
                     /translation="MQLALGATTRPWNQWSFEEACGAIAAAGYTDVAPFAHGGKIPLT
                     SESTPEEIAAVRKVVDAAGLKPSMLITHMRLSDPLEDAIADYRKTIDVAAAAGITWAM
                     NCGCSQPELYEKYNALFRACAPYAAAKGVRLVMKPHGGNGLTGQLMRAVVEAVDHPNF
                     SICYDPGNIIYYTKGEQRPETDVHAIKEHVSICIIKDCVVVDGKPDVNILPGTGWVKF
                     DSVLGSLAEAGFRGPLYVECLGGTTWDDINSRARQTYEYIADIVARL"
     gene            complement(2671..2985)
                     /gene="flgM"
                     /locus_tag="PLY56_04115"
     CDS             complement(2671..2985)
                     /gene="flgM"
                     /locus_tag="PLY56_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOJ20695.1"
                     /translation="MRIEDIASIGSTHHPEPEPPAPALRRAAPAARAEDSAEISQRAR
                     EVQKAKDAILALPETREARIAEIRARLKRGEYTISSRDIADRMFDELTSLETSDEHPN
                     QP"
     gene            3367..3657
                     /gene="gatC"
                     /locus_tag="PLY56_04120"
     CDS             3367..3657
                     /gene="gatC"
                     /locus_tag="PLY56_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOJ20696.1"
                     /translation="MRITKEYVDQVGRLSRLALADDEKERLTTHLNAILEHFERLQEL
                     DTSTVPPTAHSIPIENVFREDTPRLCLTPQEATAGAPESADDMFVVPRIVET"
     gene            3769..5226
                     /gene="gatA"
                     /locus_tag="PLY56_04125"
     CDS             3769..5226
                     /gene="gatA"
                     /locus_tag="PLY56_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOJ20697.1"
                     /translation="MPELHMLTAHELAELLRRGEVSAVEIAEDTLDRISAVEERVKSY
                     VTVAAEEALEQARAVDERRARGEALPPLAGIPIALKDNLCTRGIRTTCSSRILENWVP
                     PYDATVVKRLRDQQAVFIGKTNMDEFAMGSSTEYSAFHVTRNPWDLSRVPGGSSGGSA
                     AAVAAREATLSLGSDTGGSIRQPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPVA
                     RDVTDAALLLQAISGHDPNDATSATAEVPDYTKALRSDVKGLKLGVPRELWTQGVDPA
                     VNAAVRAAVDRLVELGAEAEEVSMPHIDYSFPVFYLIAPSEASSNLARYDGVRYGLRV
                     PADDPNTMMERTRDAGFGPEVKQRIVIGTFALSAGYYDAYYLKALKVRTLVKQDFDRA
                     FERYDLLVSPVSPTVAFGIGEKTGDPLQMKLADVCTIPINLAGIPAISVPCGFYEGLP
                     IGLQIMGKPFGEETLLRAAYAYEQSTDWHRRTPEV"
     gene            5254..>6417
                     /gene="gatB"
                     /locus_tag="PLY56_04130"
     CDS             5254..>6417
                     /gene="gatB"
                     /locus_tag="PLY56_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOJ20698.1"
                     /translation="MTEYEPVIGMEVHAELLTESKMFCGCPTTFGAAPNTQVCPICLG
                     LPGTLPVPNRRAVEFVVRAALALNCEISPRSIFYRKNYYYPDLPKAYQISQYGDDPIG
                     RNGYIEIEVDGRVKRIGIRRCHLEEDTGKLFHVSPHTSEIDYNRSGMPLMEIVSEPDI
                     SSAEEARAYLEQLRAILTYLGVCDGKMEQGSLRCEPNVSVRPKGSTALGTKTELKNLN
                     SFRAVARGIEYEIARQTALLREGGVVVQETWRWDEARQVTAPMRSKEAEQEYRYFPDP
                     DLTPMVFDPAWIEEQRAQLPELPRQKQLRFQKEYGLPAYDAQVLTETRALAEYFEAAA
                     KKHSDAKAVSNWVMGELLGRLNAASLDITESKISPEALAGLLALIDKGTISGKI"
BASE COUNT         1085 a         2060 c         2111 g         1161 t
ORIGIN      
        1 tggatgttat ccggcttgat atcgcggtgg ataacgccgc gtgcgtgcgc gtgctgaagg
       61 gcgtcgcaca cctgcagggc gacccggatg gcgcgctcga ccggcagcgg gccctccatc
      121 tggaggatat cccggagcgg ctggccctcc agatactcca tggcgatgaa gtagcgatcc
      181 ccctcgttat cgacatcgaa gatggtgacg atgtgtgggt ggggggagag ggcccccgca
      241 gcccggccct cgcgccgaaa gcgctcgatc cgctcgcgtc gcgcctggcc taccagccca
      301 gggggaatcg ccagctcctt gatggcgata cgccggcccg tttgcgggtc gtgcgcctca
      361 tagacgatgt cgttgctgcg cgctatctcc cggatgatct ggtagcgttt cagcgtgttg
      421 ggagccatgg gatccgagcc ggctaacgcc acggaagcgg gaagcggcgt cgcttctcac
      481 cctcggttgg gggagaaggg ggcgcttccg gcgcgggctc ttcgcccgga tcagcaggct
      541 cagccgccgg gtcgaagcgc ttcaccgcat ccacgcgaat gatgagtacc gtgatgttgt
      601 cacggccccc cctgtcattg gccgcgtcga tcagctccat gcaggcgacg gagggcgccg
      661 aacgcctggc gagaagggcg atctcctggt cgtcgaggtg cccggtgagc ccgtcggaac
      721 agaggagaat cacatcaccg ggccggatct tctcgcggaa gaggtccacc tccacgctct
      781 cttgcgcgcc gagcgagcgg gtgatgacgt tacggaaggg cgagcgcgct gcggattcgg
      841 gatccagcac gcccaggcgt atctgctccg cgacccagga gtgatcctgg gtgatctggc
      901 ggatctcgcc gtcgcgaatg aggtagaggc gcgagtcgcc cacctgcgcg gcgatcaggt
      961 actcctcgtg gaggatcgcc ccggtgaagg tggtgcccat ccccgagcgc ccgggaatgg
     1021 tcgcggccgt gtcgagaatg agggcgttgg catactgcac ggcggccgcc aacgccgtct
     1081 ccagatcatc ggtggtgttg gcgtaatact ttttgatgat gctcttgaga gccagctccg
     1141 aggcgatctg cccggcggca tgcccaccca tgccatcaca cacggcgtag aaccgaccgc
     1201 gcgcggccag cacggcgggc tcctccggct ccaggaagtc aaacttgtcc tcgttgtttt
     1261 cgcgtacgcg tccgaggtcg gtcttggcgg cgaacgtcac ctcggcgtgc cactcgggcg
     1321 ctcgcgcttc gagcttggcc cagccctccc gaagagactg aagctcaaga ggcgccgtga
     1381 tctcgttctc ttcgcctccg tgcctgctct ctaagggtgc actccccatg tgcggactct
     1441 ccttgccagc cggttgctac agctctcaat cggtatgata cattatctcc cgggagatgt
     1501 aaaggagaag ggggatccgg gcgggtgccc tgctctccgc aggaatcccg gatcgtgggg
     1561 gcctatctcc tcgcccccgc gatccacgga tccacccctc tcatggggcc tcaccggact
     1621 gcgaggcgcg cgccgcgctt ctccacccgg cggttacagc cgggcgacga tgtcggcaat
     1681 gtactcatag gtttgcctgg cgcggctgtt gatgtcgtcc cacgtggtgc cacccagaca
     1741 ctcaacgtag aggggcccac ggaagcccgc ttccgcgagc gagccgagca cgctgtcgaa
     1801 cttcacccag cccgtgccgg ggaggatatt cacatccggc ttgccatcga ccaccacgca
     1861 atccttgatg atgcagatgc tcacgtgctc cttgatggcg tgcacatccg tctctggccg
     1921 ctgctcgccc ttggtgtagt agatgatgtt gcccgggtca tagcagatgg agaagttggg
     1981 atgatccacc gcctcgacga ccgcgcgcat cagctggccg gtgaggccat tgccgccgtg
     2041 gggcttcatc accaggcgca cgcccttcgc cgccgcatag ggcgcgcatg cccggaagag
     2101 cgcgttgtac ttctcataca gctcgggttg gctgcagccg cagttcatcg cccaagtgat
     2161 gcccgcggcg gcggccacgt caatcgtctt gcggtaatcg gcgatagcat cctcaagcgg
     2221 atcgctcagg cgcatgtggg tgatcagcat cgatggcttg aggccggcgg catcgaccac
     2281 tttccgcacc gccgcgatct cctccggggt gctttccgag gtgagcggta tcttcccgcc
     2341 gtgggcaaag ggggcgacat cggtgtagcc ggccgcggcg atggcgccac acgcctcctc
     2401 gaagctccac tggttccagg gacgagtcgt agctcccaat gcgagttgca tggatcctcc
     2461 tcacggcgcc gatccgaggg ggcccatagg cccagcaggc gatggatacc cgcgtctatc
     2521 gataggcgct aacggagcat tcccgctatg caagcggata ccttccgggg gatcgtggcc
     2581 cgggcgcatt gcggcaggcg gactcgtgcc cggcgcattc ggctggccca cgcgcggacg
     2641 agaaccgagc tgcgcgtcgc cccggcttga ttacggctga ttgggatgct cgtccgacgt
     2701 ttccagactc gtcaactcgt cgaacatgcg gtcggcaata tcgcgcgagg agatagtgta
     2761 ctctcctcgc ttcaagcggg cgcgtatctc ggcaatgcgg gcttcgcgcg tttcgggcag
     2821 ggcgaggatg gcatccttgg ccttttgcac ctcgcgagcg cgctgagaga tctccgcgga
     2881 gtcttcggcg cgtgcggcgg gggcggcgcg ccgcagggcc ggagccggcg gttctggctc
     2941 cgggtggtgc gtcgaaccaa tggaagcgat atcctcaatg cgcatcggcg atctcccagg
     3001 gtagggtgaa ggtggcgtgg tatagggctt tccctgcgga aggggggccc atgctttcct
     3061 acccgctctt ttctaggttg gccctcgcgt gccactttcc tgcttcggtc cggcaacttt
     3121 cctaaccccc ggctccttcc ctgccttccg accctcgcct cccccagtga aggggagcta
     3181 ccgcgcggca gtggcggcgg tctccaagga ctctgatgct tgagccgcag gttcctatgc
     3241 ctcgtgacta gactccttac cgcggaatcc cgaaataagg ataggttgcc gcgcgatctg
     3301 cgattttgct ataatggagg tggcgcccgc gtgagaagcg cgccgggcgc cggggagagg
     3361 atctgtatgc ggatcaccaa agagtatgtc gaccaggtgg gccggctctc gcgtctggcg
     3421 ctcgccgacg acgaaaagga acgcctgacc acgcatttga acgccatcct cgagcacttc
     3481 gagcgcctcc aggagctgga cacgagcacg gtgccgccga cggcgcacag catcccgatt
     3541 gagaacgttt tccgggaaga cactccgcgg ctctgcctga cgccccaaga ggcgaccgcc
     3601 ggcgccccgg agagcgccga tgatatgttt gttgtgccgc gaatcgtcga gacgtaacgc
     3661 ttgacgcatg agacgggccg cccgagacgg gtggctggcc caagcgctga ctcctgcagt
     3721 tggccattcg cggcggatcc tgtttcgtgt atgatgttgg agtaacccat gccagaacta
     3781 catatgctca ctgcccacga gctcgccgag ctgctgcgcc ggggcgaggt ttcggcggtg
     3841 gagattgccg aggatactct ggaccgcatc agcgccgttg aggagcgggt gaagagctac
     3901 gtcacggtcg cggctgagga agcgctcgaa caggcgcggg ccgtggatga gcgcagggcg
     3961 cgcggcgagg cgctgccgcc tctggccggc attccgattg ctctcaaaga caacctctgc
     4021 acccggggca ttcgaaccac gtgctcgtcg cggatcctcg aaaactgggt gccgccctat
     4081 gacgccaccg tggtaaagcg cctgcgtgac cagcaggcgg tcttcatcgg gaagaccaac
     4141 atggacgagt tcgcgatggg ctcgtccacc gagtactcgg cgttccacgt cacgcgcaat
     4201 ccgtgggatc tttcgcgcgt gccaggaggc tccagcggtg gctcggccgc ggcggtcgct
     4261 gcgcgcgagg caacgctctc cctcggctct gataccggcg gctccatccg acagcccgct
     4321 tccttctgcg gggttgtcgg cctgaagcca acctacgggc gtgtttcgcg ctatggcttg
     4381 gtcgccttcg cgtcgtcgct cgatcagatc gggccggttg cccgcgatgt gacggatgcc
     4441 gccctcctcc tacaggcgat cagcggccac gatccgaacg acgcgacctc agccacggcg
     4501 gaggtgccag actacacgaa ggccctgcgt agcgatgtga aggggctgaa gctgggcgtg
     4561 cctcgggagc tttggacgca gggcgttgac ccggcagtga atgccgccgt gcgcgcggcc
     4621 gtcgaccgcc tggtggagct cggcgccgaa gccgaggaag tctcgatgcc gcacatcgac
     4681 tactccttcc cggtattcta cctcatcgcg ccgagcgagg cgagcagcaa tctggcccgc
     4741 tacgatggcg tgcgctacgg cctgcgcgtg cccgcggatg acccgaacac gatgatggag
     4801 cgcacgcgcg acgccggttt tggcccagag gtgaagcagc ggatcgtgat cggcaccttc
     4861 gcgctctccg ccgggtatta cgatgcctac tacctgaagg cgctgaaggt gcgcaccctc
     4921 gtgaagcagg atttcgaccg tgctttcgag cgctatgacc tgctcgtatc gcccgtgtcg
     4981 cccacggtgg cttttgggat cggcgagaaa acgggagacc cgctgcagat gaagctggcg
     5041 gatgtctgca ccatccccat caacctggcg ggaattccgg cgatctctgt gccctgcggc
     5101 ttctacgagg gccttcccat cggcctgcag atcatgggca agccttttgg cgaggagacc
     5161 ctcctccgcg cggcctatgc ctacgagcaa tccaccgact ggcaccgccg cacgcccgag
     5221 gtgtgacccg cggctcgata gttcggagga acgatgacag agtatgaacc ggtaatcggc
     5281 atggaggtcc atgccgagct gttgaccgag agcaagatgt tctgcggttg cccgacgacg
     5341 ttcggtgcgg cgccgaacac acaggtgtgt ccgatctgcc tggggttgcc gggcaccctg
     5401 cccgtgccaa accggcgtgc cgtcgagttc gtcgtgcgcg cggcgctggc gctcaactgc
     5461 gagatctcgc cacggagcat cttctaccgc aagaactact actaccctga tctccccaag
     5521 gcttaccaga tctcgcagta tggcgacgac ccaattggcc gcaacggcta catcgagatc
     5581 gaggtggacg gccgagtgaa gcgcatcggc atccggcgct gccatctgga ggaggatacg
     5641 gggaagctct tccacgtctc gccgcacacg agcgagatag actacaaccg ctctggcatg
     5701 ccccttatgg agatcgtctc cgagccggat atctcctcgg cagaggaagc gcgtgcctac
     5761 ctggagcagc ttcgagctat cctgacctac ctcggcgtct gcgacggcaa gatggagcag
     5821 gggagcctgc gctgcgagcc caacgtctcc gttcgcccca agggtagcac ggctctgggc
     5881 accaagacgg agctgaagaa cctcaactcc ttccgcgccg tcgcccgcgg gattgagtat
     5941 gagattgccc gccagactgc tcttctccgc gagggcggcg tggtggtcca ggagacgtgg
     6001 cgctgggatg aggcgcgcca ggtgacggcg ccgatgcgca gcaaagaggc ggagcaggag
     6061 tatcgctact ttcccgatcc tgacctgacg ccgatggtgt tcgatccggc gtggatcgag
     6121 gagcagcgcg cccagcttcc ggagctgccg cgccagaagc agctccgttt ccagaaggag
     6181 tacgggttgc ccgcctacga tgcccaggtc ctgaccgaga ctcgagccct cgcggagtac
     6241 ttcgaggcgg ctgccaagaa gcactccgat gccaaggcgg tgagcaactg ggtgatgggc
     6301 gaattgctgg gccgactgaa cgccgccagc ctcgacatca ccgagagcaa gatctcgccc
     6361 gaggcgctcg cgggcctgct ggcgctgatc gacaagggaa ccatcagcgg caagatc
//
LOCUS       DAOSCV010000112         6393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7753_length_6393_cov_3.054907, whole genome shotgun sequence.
ACCESSION   DAOSCV010000112 DAOSCV010000000
VERSION     DAOSCV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7753_length_6393_cov_3.054907"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PLY56_04135"
     CDS             <1..1167
                     /locus_tag="PLY56_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20699.1"
                     /translation="ARFGTVNEDLMETGVRASSLVAGMGPLMMLLVNLGVVAALWWGG
                     FLVHGGKMQVGQILAFTNYLTQMLASLMMVGMLLMQLSRAGASADRILEVLETEPAVC
                     DVPDARPGTVVTRGRIEFDNVEFSYGGAGSQPVLRGVSFVIEPGECVVILGATGSGKS
                     TLVNLITRLYDVTAGRILVDGVDVREMRQEELRAAIGVVPQETVLFSGSVRDNIRFAR
                     PESTDAEIEEAARLAQARAFVEALPGGYDAMLGQRATSLSGGQKQRLAIARALACRPA
                     ILILDDCTSAVDATTEAEILRGLQTWDHRCTRLIVAQRASAVFLADRILILDEGKVAA
                     FGTHEELLRTSPIYREILRSQLGEFEDAETPPLPSLQESAPCLVAGPSGEGGEA"
     gene            1164..3020
                     /locus_tag="PLY56_04140"
     CDS             1164..3020
                     /locus_tag="PLY56_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20700.1"
                     /translation="MREKRVPPGMMPFGHGPGRHHPGGPVQKAADTRGVLRRLWGYLR
                     PYALRLVGVSLLVVATTALSLLHPYLTSRAVDDGIVPGNLHALARVAGWMLLVHVLAA
                     AGTYVQSIAMIRISQGTVRDLRRDLFTRIQALPLRFFDRNPHGELMSRLTNDTEAVSQ
                     TLAQSVTRLVSSTLSVIGAAYAMFALNVRLAVAALITLPLMLVASRAIARATREGFRE
                     RQSCLGAVNAIVEEMTTGQRVVKLCRHEHQAIAQFDEANAALRRAGIKAGIWVGLMGP
                     VMGLFRNAGFAILAAAGGWMVLRDWATLGIVAAFIAYAQHFSRPLNEIAMIYGTIQSA
                     VAGAERVFAILDEAPERDAPERERELADVRGEVVFDRVSFSYVEGQPILKDVSFCALP
                     GQTIALVGPTGAGKTTIINLLTRFYDIDSGSIRVDGYDIRDVTRESLRRSLGIVLQDT
                     FLFADTVRENIRYGRLDATDEEVEEAARLANAEGFIRRLPQGFDTPLSEAAGTLSQGQ
                     RQLLAIARAILADPAILILDEATSSVDTRTELQIQEAMLRLMDGRTSFVIAHRLSTIR
                     QADCILVIDGGRVVERGTHEELLAAGGAYYALYSSQYQLSAKPLRTAESAPA"
     gene            complement(3528..4319)
                     /locus_tag="PLY56_04145"
     CDS             complement(3528..4319)
                     /locus_tag="PLY56_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20701.1"
                     /translation="MRNLRWIAIAAALVAAANLARAQGPGKSEAGRPAPSPEMRQRME
                     QFRKFREEHKYTFQLTDTIVALGELEKQNRHALTPAQAKKVNAVLAKVRKQPKLTQEQ
                     AKQALKEVKAPLTERQLTEVGKITKEMSERRGRPDGAGPRPGGRPDGAAARPGGQRPG
                     GRPDGDRPGARRPGDRPDGAASRPGGQRPGGRPDGAGQRPGGDRPGGRPDGDGPGARR
                     PAFDPARMKDFNPLAAPSGEGAARPRGNRRLQEAVSLIEKKATKK"
     gene            complement(4343..5557)
                     /locus_tag="PLY56_04150"
     CDS             complement(4343..5557)
                     /locus_tag="PLY56_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ20702.1"
                     /translation="MNLWESMRVALTGLAANKMRSALTMLGVIIGVAAVIAMMAIAQG
                     ARADTMKRIQAMGTNSLMVFAGQSRQGRVRGGFGSSQSLTVDDAEAIAKLGPPIARVS
                     PEVGQSAQVKYRNANTNVSIQGVGADYCEIRNVTVERGRFFSESDVLAMRRVCVIGPD
                     TATNLFGQQNPVGKRIRIAGSGFLVLGVTKAKGSQGWTNPDDQIWVPYTTAMRRLFGL
                     DYLRMISVQVTEMKRSDEAIEMITRLLRRRHRISAGGEEDFFVRSQAEMMEAAEETSR
                     TFTLLLAGIASVSLLVGGIGIMNIMLVSVTERTREIGIRKALGARARDILGQFLIEAM
                     VLSITGGLVGVILGVGGSLLLANSAGWTTIIAPQSILLAFGFAGAVGIFFGSYPAHKA
                     SRLSPIEALRYE"
     gene            complement(5554..6285)
                     /locus_tag="PLY56_04155"
     CDS             complement(5554..6285)
                     /locus_tag="PLY56_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20703.1"
                     /translation="MIEIRDIRKTYRMGEVSVHALRGITLTIDAGDMVAIMGPSGSGK
                     STLMNILGCLDTADEGSYQLDGTDVGSLDDDGLAAIRNRKIGFVFQKFNLLPRTTALH
                     NVEVPMLYAGMPRAQRAERAKWALEVVGLADRMHHQPNELSGGQQQRVAVARALVNDP
                     VILLGDEPTGNLDTRTGEEIMAIFQDLNRQGKTVILVTHEPDIARHCKRVVRFRDGQI
                     LSDERVSEPLDAVEVLRQLPVEDEQ"
BASE COUNT          957 a         2154 c         2168 g         1114 t
ORIGIN      
        1 gcccggtttg gaacggtgaa cgaggatctg atggagacgg gggtgcgcgc ctcctcgctg
       61 gtggcaggga tgggcccgct gatgatgctc ctcgtgaacc tgggcgttgt ggccgcgctc
      121 tggtggggag gcttcctggt gcatggcggc aagatgcagg tgggacagat ccttgccttc
      181 acgaactacc tgactcagat gctcgcctcg ttgatgatgg ttgggatgct cctgatgcag
      241 ctcagccgcg cgggcgcctc cgcagatcgg atcctcgagg tgctggagac ggagcccgcg
      301 gtgtgcgacg tgcccgacgc ccggccagga actgtggtga ctcgcgggcg catcgagttt
      361 gacaacgtgg agttcagcta tggcggcgcc ggttcccagc ccgtcttgcg cggcgtctcc
      421 ttcgtcatcg agccgggcga gtgtgtggtc attttgggcg cgaccggatc gggcaagtcc
      481 accctcgtca acctgatcac ccggctatat gatgtgaccg ccggccggat cctggtggat
      541 ggggtggacg tgcgcgagat gcgccaggag gagctgcgcg ccgcaatcgg cgtcgtgccc
      601 caggagacgg tgctcttctc ggggagcgtg cgcgacaaca tccggtttgc ccgcccggaa
      661 tcgactgacg ccgagatcga ggaagccgcg cgcctggcgc aggcgcgcgc cttcgtcgag
      721 gcgctccccg gggggtatga cgcgatgctc gggcagcggg ccaccagcct ttctggaggc
      781 cagaagcagc gcctggcgat tgcccgcgcc ctcgcgtgcc gcccggccat tctgatccta
      841 gacgactgca cgagcgccgt ggatgccacg acggaagcgg agatcctgcg ggggcttcag
      901 acgtgggacc atcgctgcac ccggctgatt gttgcgcaga gggccagcgc cgtcttcctg
      961 gcggaccgga ttctgatcct ggacgagggg aaggtggcgg cgttcggcac gcatgaggag
     1021 ctactccgca ccagcccaat ctaccgcgag attcttcgat cgcagctggg agagttcgag
     1081 gatgcggaaa cgccgccgtt gccctctctc caggagagcg cgccctgcct ggtcgcgggg
     1141 cccagcgggg aagggggaga ggcatgagag aaaaacgtgt accgcccggc atgatgccgt
     1201 ttgggcacgg tcccgggcga caccacccag gcggcccggt tcagaaggcg gcggatacgc
     1261 gcggcgtgtt gcgccggctg tggggctatc tgcgcccgta cgccctccgg cttgtcgggg
     1321 tgtcgctcct ggtcgtggcc accacggcgc tctcactcct ccacccgtat ctgaccagcc
     1381 gggcggtgga cgacggcatc gtgcctggca acctgcacgc gctggcgcgc gtggccggct
     1441 ggatgctcct ggtgcacgtg ctcgccgcgg cgggcaccta tgtgcagtca atcgcgatga
     1501 tccggatctc gcaaggtacg gtgcgtgacc tccggcgcga tctctttacc cggatacagg
     1561 cgctgccctt gcggttcttc gaccgcaacc cgcacggcga gctgatgagc cggctgacga
     1621 acgacacgga ggctgtcagc cagacgctcg cgcagtcggt gacgcgcctt gtctcgagca
     1681 cgctctcggt gatcggagcg gcctacgcga tgttcgcgct gaacgtgcgc ctggcggtcg
     1741 cggcgctcat cacccttccc ttgatgctgg tggcgagccg tgccatcgcc agggcgacgc
     1801 gcgagggctt ccgcgagcgc caaagctgcc tgggcgccgt gaacgccatc gtcgaggaga
     1861 tgacgacggg ccagcgggtg gtgaagctgt gccggcacga gcaccaggcg atcgcgcagt
     1921 tcgacgaggc gaacgccgcg ctccgccgcg ccgggatcaa agcggggatt tgggtgggcc
     1981 tgatgggccc cgtgatgggt ctcttccgca acgccggctt tgccatcctc gccgcagcgg
     2041 gcggctggat ggtactgcgc gactgggcga cgctcggcat cgtcgccgcg ttcatcgcgt
     2101 atgcccagca cttctcgcgc ccgctgaatg agatcgcgat gatctacggc acgatccagt
     2161 cggccgtcgc cggcgccgag cgcgtcttcg ctatcctgga cgaggctccc gagcgcgacg
     2221 cgccggagcg cgagcgcgag ctcgccgatg tgcgcgggga agtcgtcttc gaccgcgtca
     2281 gcttcagcta tgtcgagggg cagccgatcc tgaaggacgt ctcattctgc gccctgcctg
     2341 ggcagacaat cgcgctcgtg ggcccgaccg gcgccgggaa gacgacgatc atcaacctcc
     2401 tcacgcggtt ctacgatatc gactccggga gcattcgcgt ggacggctac gatatccgtg
     2461 acgtgacgcg cgagagcctg cgccggtcgc tcgggatcgt gctccaagac accttcctct
     2521 tcgcggacac ggtgcgcgag aatatccgtt acgggcgcct ggacgcgacg gatgaagagg
     2581 tggaagaagc cgcccggcta gcaaacgccg agggctttat tcgccggctg ccccaggggt
     2641 tcgacacgcc gctttccgag gccgccggca cgctcagcca gggccagcgc cagcttctcg
     2701 ccatcgcgcg cgcgatcctg gccgacccgg cgatcctcat cctcgacgag gcgacgagca
     2761 gcgtcgacac gcgcacggag ctccagatcc aggaggcgat gctccggctg atggatgggc
     2821 ggacgagctt cgtgattgct caccggctga gcaccatccg ccaggccgat tgcatcctgg
     2881 tgatcgacgg cgggcgcgtc gtggagcggg gtactcacga ggaactcctg gccgcgggcg
     2941 gcgcctacta cgcgctctat tcgagccagt accagctttc ggcgaagccg cttcgcacgg
     3001 cggaaagcgc gccggcgtag agggggccgg gcgtgattcc gagatgagga atcaactcgt
     3061 gggcataaag acgctgggca cgaatgttgg agctctccgg catcctcggc catagccccg
     3121 caggcggccc tgctccggcc ctgaaagggc cgtataactt ggcccagggt gtctgcaccc
     3181 tgggccatgt gctggaaaaa taaccacgac cgcgaagagg tcgtgcaatc cgctaccacg
     3241 tattgtcgca ccctttcagg gtgctaggta ttttctgcct gcgagactga gggtcaggag
     3301 accctgggct agaatatggg accgcttcgt ggtcctctga gatggcggcg cgggtagctc
     3361 aaccacgctt ccggctgggg cgcgcaaccc cgggtgcgcc cactcggcgc atctgtgtgc
     3421 gtacccttca tccaaggtat cgacaccggg gcggaaccgg tagcgtgaaa tggggagagg
     3481 ggccccgcag ggcccctctc cccgttgagg agtgaatggc gtggcgccta cttcttggtg
     3541 gccttcttct cgatgagcga gaccgcctcc tggaggcgcc ggttgccgcg cggccttgcg
     3601 gcgccctcac ccgagggggc cgcgagcggg ttgaagtctt tcatccgcgc ggggtcgaag
     3661 gctggacgcc gggcaccggg cccatcgcca tcgggccggc cgccggggcg gtcgccgccg
     3721 gggcgctgcc cagccccatc gggccggccg ccgggacgct ggccacccgg acgggatgcg
     3781 gctccatcgg gccggtcgcc aggacgccgg gcaccggggc ggtcgccatc gggccggcca
     3841 ccgggacgct ggccaccggg gcgagcggcg gcgccatcag gtcggccacc ggggcgcgga
     3901 ccggctccat ccggccggcc acggcgctcg ctcatctcct tggtgatctt cccaacctcc
     3961 gtcagctggc gctcggtgag cggcgccttc acctctttga gcgcttgctt tgcctgctcc
     4021 tgagtcagct tgggctgctt gcgcaccttc gcgaggacgg cgttaacctt tttcgcctgc
     4081 gcgggcgtga gcgcatgccg gttctgcttc tccagttcgc ccagggcgac gatcgtgtcg
     4141 gtgagctgga acgtgtactt gtgctcctcg cggaatttgc ggaactgctc catccgctgg
     4201 cgcatctcgg ggctcggagc cggtctcccg gcttctgact tgcccggccc ctgcgcgcgg
     4261 gccaggttgg ccgcggccac cagggccgcc gcgatcgcga tccaacgcag gtttctcatc
     4321 tgtttctccc tctcccgttc gtctactcat agcgcagcgc ctcgatcggg ctgaggcgcg
     4381 atgctttgtg cgcggggtaa ctgccgaaaa agatcccgac ggcgccggca aacccgaacg
     4441 ccaggaggat gctctggggc gcaatgatcg tcgtccaccc ggcggagttg gcaagcagga
     4501 gagagccgcc aacgcccagg attactccca ccaggccgcc ggtgatgctg aggaccatcg
     4561 cttcgatcag gaattggccg aggatgtcgc gggcgcgcgc gcccagcgcc ttccggatgc
     4621 cgatctctcg cgtgcgctcg gtcaccgaga cgagcatgat gttcatgatc ccgatcccgc
     4681 caaccaggag cgagacgctc gcaatccccg ccaggaggag cgtgaacgtc cggctggtct
     4741 cctcagcggc ctccatcatc tcggcttggc tgcgcacgaa gaagtcctct tcgccgccag
     4801 ccgagatgcg gtgccggcgg cgcaggagcc gggtgatcat ctcgatcgcc tcgtccgagc
     4861 gcttcatctc cgtcacctgc acgctgatca tacgcaggta atccaggccg aagaggcggc
     4921 gcatcgcggt ggtgtagggc acccagatct ggtcatcggg gttcgtccag ccctgggagc
     4981 ccttggcctt cgtgacgcct agcaccagga agccgctgcc cgcgatccgg atccgcttgc
     5041 ccaccgggtt ttgctgtccg aacaggttcg tggccgtatc cgggccgatg acgcaaacgc
     5101 gccgcatcgc caggacgtcg ctttccgaga agaagcgccc gcgctcgacg gtgacgttgc
     5161 ggatctcgca gtagtcggcg ccgacgccct gaatcgagac gttggtgttg gcgttgcgat
     5221 acttcacttg cgcggactgg cccacttcgg gtgagacgcg cgcaatgggc ggcccgagct
     5281 tcgcgatcgc ctcggcatca tccaccgtga gcgactggct gctgccgaac ccgcctctca
     5341 cccggccttg ccgcgattgc ccggcaaaca ccatcaggct gttggtgccc atcgcctgga
     5401 tgcgcttcat cgtgtcggcg cgcgcgccct gggcaatcgc catcatcgca atcaccgccg
     5461 cgaccccgat gatcacaccg agcatcgtca gcgccgagcg catcttgttc gcggcgagac
     5521 ccgtgagggc cacgcgcatg ctctcccaca ggttcattgt tcgtcctcca cgggcagctg
     5581 gcgcagcact tccacggcat cgaggggctc actcactcgc tcatcgctga gaatctggcc
     5641 gtcgcggaag cgcaccacgc gcttgcaatg cctggcgatg tcgggctcgt gtgttacgag
     5701 gatcaccgtc ttcccctggc ggttgaggtc ctggaagatc gccatgatct cctcgccggt
     5761 gcgcgtgtcc aggttgccgg tgggctcgtc accgagcagg atcaccgggt cgttgacgag
     5821 cgcccgggcg acggccacgc gctgttgctg cccgcccgag agctcgttcg gctggtgatg
     5881 catccggtcc gccagcccca ccacctccag cgcccatttg gcgcgttccg cccgctgcgc
     5941 gcgcggcatg cccgcataga gcatcggaac ctccacgttg tgcagcgctg tggtgcgagg
     6001 cagcaggttg aatttctgga agacaaagcc gatcttccgg ttgcggatcg ccgccaggcc
     6061 gtcgtcatcc aggctgccca cgtcggtgcc atccagctgg tagcttcctt catccgccgt
     6121 atccaggcac cccaggatgt tcatcagcgt cgacttgccc gatccggaag gacccatgat
     6181 agcgaccata tcgcccgcgt cgatggtgag cgtgatcccg cgcagggcat gcacggagac
     6241 ctctcccatt cggtacgtct tgcggatgtc gcgaatctcg atcatcggct ccttcttcct
     6301 accgcccgcg cggcatcccg cccatcggac cgcggcggcc tccgggcccg ccggggcctc
     6361 cgccccgacc ctgcgggccg cgcccgggcg ctc
//
LOCUS       DAOSCV010000113         6361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7794_length_6361_cov_3.273708, whole genome shotgun sequence.
ACCESSION   DAOSCV010000113 DAOSCV010000000
VERSION     DAOSCV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7794_length_6361_cov_3.273708"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1152)
                     /locus_tag="PLY56_04160"
     CDS             complement(34..1152)
                     /locus_tag="PLY56_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ20704.1"
                     /translation="MSWIAARLDARAAEVRLAWIIRMREEGILARMPDEELSAQAERV
                     QAAWQQYLASAGTGEVGGEPDWGEVRRALAGWSIEDALGALLALRAVCRDLLATEDAG
                     RPEDAIATFERIADRFITGVISQGVVGQNLQHESECSDRQQAEVLQRVIAAQEEERKR
                     IARELHDQTGQSLTSLMIGLRRLEAAQMPEEERQAIAELQQNANRALDELHHLIFERR
                     PSVLEDLGLVVALQRYIEEFARRTGLRVETELDALAGVRLEPAVEIALYRIVQEALTN
                     VDRHARATTASVLLQCRGDSLLLIVEDDGSGFVVPEIGAGDSEHFGLMGMQERAALIG
                     GKLTIESSPGAGTTIYVEVPRPGCKERSAAPSADPRAS"
     gene            complement(1334..2530)
                     /locus_tag="PLY56_04165"
     CDS             complement(1334..2530)
                     /locus_tag="PLY56_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOJ20705.1"
                     /translation="MPAVEVRPGIYWAGVNDRTSDLFEGFWPVAREGVSHNSYLIDDE
                     KKALIDITSESLLESLLSQIATVTDPAGLDYIVINHMEPDHTGALRALRRLAPRATLL
                     GSKKAVEMMESYYGVTEGVRAVEDGETLSLGRNTLQFFSTPFVHWPETIMTYAQEQKV
                     LFSCDGFGGYGALQGSIFADEYADLRFYEEEALRYYTNIVALFGKQVLKAIDRLKDLP
                     VEVVAPSHGLVWRRNPGRIIELYRTWAGYATGPVEPGVTVVFGTMYGNTRAMAEAVAA
                     GVAGAGLPVTLFDAARVHPSYILPAIYSRQGVVIGSATYEGGLFPPVRNLLSLVESKR
                     IVGRTAAFFGSRGWGGGARREFARQAETLQWEVVDAWEMQGGPTAKDLQEGEEFGHRF
                     AAGLRR"
     gene            complement(2816..3748)
                     /locus_tag="PLY56_04170"
     CDS             complement(2816..3748)
                     /locus_tag="PLY56_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOJ20706.1"
                     /translation="MAVLRARFQAGDLVLFLGAGVSLSAGLPGWESLLHRLAVEMWAQ
                     RLGADAGGEDAEEFARLFRHAFGGFPIISGRYIRKALGHAFQERVRAILYSHPDATSS
                     LLKAVTRLCVSSGSGAGIRAVVCYNYDDLLETHLAEGGVSFCSVSAEGQSPPPQVLPI
                     YHVHGFLPRDGSLDHQSEIVFAEDAYHTQFLDPYAWSNITQLNLLRERTGLFVGFSMT
                     DPNLRRLLDVANRYRHGRPHYVIMRTPSREQIAFGDTSQVSDDRLEDFIGAFRSVQED
                     ALADLGLHVVWVEHFDEIPCLLDSIRESAPDSTP"
     gene            complement(3971..4600)
                     /locus_tag="PLY56_04175"
     CDS             complement(3971..4600)
                     /locus_tag="PLY56_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ20707.1"
                     /translation="MKDRWISRLIELASDSPYEVAHDLFAGQPGPPLPNGVEERVQQA
                     VRWGLQERELEKYERELSRRTTLGEFLSWARTRKGCTEEELGQRAGISAECIRALEKD
                     QICPLDVAPEHVARVAAVLDVSVFTLLERLQAARLDSESRKDRGGRARPPSADPSVVW
                     SSGARRARQVMEFLCLRRKERQPVTQADLDHYCGAVYRAWKEQREDPAE"
     gene            complement(4864..5562)
                     /locus_tag="PLY56_04180"
     CDS             complement(4864..5562)
                     /locus_tag="PLY56_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ20708.1"
                     /translation="MQYEGTDDFLVQGLREGDNLAAQQLIDTYSAPLYRYLLRRCSCD
                     EDASDVLSMVFQKVVASIHTYDPARGSFKNWLYEVAQSCRADFYRRWGGVIAGELPPD
                     DLLRSEWGPTVYYDSYPETRAWQYASHQDEGEGPEESKIIAAVQEVLTSMPKRYAEVL
                     QLEQTDLTREEIAILMGITRDHLRVLLSRAIACFKRLAQQNLVLAEWLDRAEPLPQEE
                     APRRRGRRKAFAEL"
     gene            5961..>6361
                     /locus_tag="PLY56_04185"
     CDS             5961..>6361
                     /locus_tag="PLY56_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ20709.1"
                     /translation="MTDLGGVFPALLTPFDRQGQLNEPMVEALIHHLLEAGVDGFYVG
                     GSTGEGLLMSPQERRRLTTAAVRAANGKAKVIVHVGANATDDAVDLARHAAEAGADAV
                     STLPPLFFKVGDDAGGNAPRRVKDIDHLRQQG"
BASE COUNT         1149 a         2188 c         1951 g         1073 t
ORIGIN      
        1 ttagctcagt atacaacagg cgcgggcagg gcgtcaagac gcgcgcggat ccgcagaagg
       61 tgccgcgctc ctctccttgc atccaggccg aggcacctcg acgtagatgg tcgtccctgc
      121 tccgggcgag gactcgatgg tgagcttgcc cccgatgagg gcggcgcgtt cctgcatccc
      181 catcagcccg aaatgctcgc tatcgccggc gccaatctca ggcacgacaa agccgcttcc
      241 atcatcctcc acgatgagga gcagcgaatc gccacggcac tgcaggagta cgcttgcggt
      301 cgttgcgcgc gcgtgcctgt ccacgttcgt caacgcttcc tggacgatcc ggtagagggc
      361 aatctctacc gctggttcca ggcgcacacc ggccagcgca tccagctccg tctccacgcg
      421 gagccctgtc cgccgcgcga actcctcaat gtagcgctgg agcgcaacga ccagaccaag
      481 atcctccaga acgctggggc gtcgctcgaa gatcaggtga tgcagctcat ccaacgcgcg
      541 attcgcattc tgctgcaact cagcgatcgc ctggcgctcc tcctccggca tctgcgccgc
      601 ttccagacgg cgtaagccga tcatgagcga ggtgagcgac tgcccggtct ggtcgtgcag
      661 ctcgcgggca atccgctttc gctcctcttc ctgggcggca atcacccgtt ggagcacctc
      721 cgcttgctgg cggtcgctgc actcactctc gtgctggaga ttctgcccga ccacaccctg
      781 cgagatcacc ccggtgatga accgatccgc gattcgctcg aaagtggcga tggcatcctc
      841 cggccgcccc gcgtcctccg tggcgagaag gtcgcgacag accgcgcgca gcgccagcag
      901 tgcgcctagc gcatcctcga tgctccagcc tgccagcgcg cgccgaacct cgccccaatc
      961 gggctcgccg cccacttcgc cggtgcccgc ggaagccagg tactgctgcc aagccgcttg
     1021 cacgcgctcc gcctgggccg atagctcctc gtccggcatc cgggccagga taccctcctc
     1081 gcgcatgcgg atgatccagg ccaggcgcac ttccgccgcg cgcgcatcga gccgcgccgc
     1141 aatccaggac acggccccct ccgacggtcg cgatgccctc tcgccggcaa gaccgaccaa
     1201 ctcgtcatcc tctcccgttc aagatccgtg ttcagggata ctcccgatag gtacccattc
     1261 ccccagatag gggaaaaccg aatcgcggca cctcggcggg ggccaaagca gcacggccgg
     1321 gcggatggcg cggctatctc cgcagccccg cggcaaagcg atggccgaac tcctctccct
     1381 cctgcagatc cttggcggtg gggccgccct gcatctccca ggcatccacg acttcccact
     1441 gcaacgtctc cgcctggcgt gcgaactccc tgcgcgcgcc gccgccccaa ccgcggctgc
     1501 cgaagaacgc cgccgtgcgc ccgacaatcc gctttgactc caccaggctg agaaggttgc
     1561 gcaccggggg gaagaggcca ccctcgtagg tggccgagcc gatcactacg ccctgtcgcg
     1621 agtagatcgc ggggagaata tagcttggat gaacgcgcgc cgcatcaaag agggtcacgg
     1681 gcaggccggc gccggcaacc cccgcagcga ccgcctcggc catggcgcga gtgttaccgt
     1741 acatcgtgcc gaagaccact gtcacacccg gctcgaccgg ccccgtggca tacccagccc
     1801 acgtgcgata cagctcgata atccgtccag ggttccggcg ccacaccaat ccatgagagg
     1861 gagccacaac ctcgaccggc aaatctttca gccggtcgat tgctttgagc acctgcttgc
     1921 cgaagagggc cacaatgttg gtgtagtagc gaagcgcctc ctcctcgtag aagcgcaggt
     1981 ctgcgtactc atctgcaaag atactgccct gcaacgcgcc ataaccgcca aagccgtcgc
     2041 aggagaagag caccttctgc tcctgcgcgt aggtcataat cgtctccggc cagtgaacga
     2101 agggcgtgga gaaaaactgg agcgtgttcc ggccaaggga gagcgtctct ccatcttcca
     2161 cagcccggac accctccgtc accccgtagt acgactccat catctccacg gccttcttag
     2221 agccgagaag ggtcgcgcgg ggtgccaggc gccggagcgc acgcagtgcg ccggtgtggt
     2281 ccggctccat gtggttgatg actatgtaat ccaagccggc aggatcggtc accgtggcga
     2341 tctggctgag gagcgactcg agcaggcttt cgctggtgat atcgatcagc gccttcttct
     2401 cgtcatcgat cagataggag ttgtgtgaca ccccctcgcg ggccacgggc cagaagccct
     2461 caaagagatc agacgtgcga tcgttcactc ccgcccaata gataccgggt ctcacttcaa
     2521 cagcgggcat gtatctctcc cctcctagct tccaggtgcg cgtgtcggcc aaatcaggtt
     2581 gtcatcgcag ggcatggaaa ggccaaagcc tctctgcccc ccgtatgata gcacaggggc
     2641 agccagacat gcaaatcgcg gcgccctccg cgtgacgatt tcgggcgaag gtagccccgc
     2701 ggttcagggg gcgagggctt gcgcgggctg cgagagggga ccacttcgcg atgcgaattg
     2761 gctccacccc tatccaggag ggccagccgc tctcctgttt tgcggccact ccaggctacg
     2821 gcgtgctgtc tggcgcgctc tcgcgaatgc tgtcgagcag gcaggggatt tcgtcgaaat
     2881 gctccaccca caccacgtgc aggcccagat cggccaacgc atcttcctga acgctgcgga
     2941 aggccccgat gaagtcctca aggcggtcat cactcacctg gcttgtatcg ccaaaggcaa
     3001 tctgctcgcg cgagggggtg cgcatgatga cgtaatgggg ccgcccatgg cgatagcggt
     3061 tggcgacgtc gaggagacgg cgcaggttgg ggtcggtcat cgagaagccg acgaagaggc
     3121 cggtgcgctc gcgcaggagg ttgagctggg tgatgtttga ccaggcgtag gggtcaagga
     3181 actgcgtgtg gtaggcatcc tcggcgaaga cgatctcgct ctggtggtcg aggctcccat
     3241 cgcgagggag aaagccatgc acgtggtaga ttgggaggac ttggggtggc ggcgattgcc
     3301 cctcggcgct tacgctgcag aagctcacgc caccctcggc caggtgggtc tccaggaggt
     3361 catcgtagtt gtagcagacg acagcgcgga tgccggcgcc actccccgaa gacacgcaga
     3421 ggcgcgtgac ggccttcaac aggctggatg tggcatccgg atgcgagtag aggatggcgc
     3481 gcacccgctc ctggaaagca tggccgagcg ccttgcggat gtaacgcccg gagatgatcg
     3541 gaaagccgcc gaacgcatgt cggaaaaggc gcgcgaactc ctccgcgtcc tctcccccgg
     3601 catcggcgcc gaggcgttga gcccacatct ccacggcgag ccggtgcagc aacgattccc
     3661 aaccaggaag cccggccgaa agcgatacgc ccgcgccaag gaagagcacc aggtctcccg
     3721 cctgaaagcg agcccgtagg acggccatcg gctcgcctgc cgggtcggat atcggctcgg
     3781 ctctcccgca tggatgcgcc acggcagcct acccctctct cgcccgcgcg ccgcacgccc
     3841 atgctgctcc aggacgctcg ctctcccacc agaccgtcct tgaaacaggc gaaagcagca
     3901 cggcgggccg gtgcgagccc cggaaggggc actgtcagcc aggggctgct ctggcgcgcg
     3961 cgcacgctcg ctactccgca ggatcctcgc gctgttcttt ccaggcccga tagacggcac
     4021 cacagtagtg gtccagatcg gcttgggtta caggctgccg ctccttacgt cgcaggcaaa
     4081 ggaactccat cacctggcga gcgcgccggg cgccactcga ccagacaacg ctcggatccg
     4141 cgctgggggg ccgtgcccgc ccgccccgat ctttcctgct ctcgctgtcg aggcgcgcgg
     4201 cctgtaaccg ctccagcagg gtgaagacag agacatcgag aaccgccgcg acccgcgcga
     4261 cgtgctctgg cgcgacgtcg agggggcaga tctggtcttt ctcgagggcg cggatgcact
     4321 ccgcgctgat gcccgcgcgt tgtcccaact cttcctcggt gcaccccttc cgagtgcgcg
     4381 cccacgagag gaactcgccc agcgtcgtcc gacgcgagag ctctctctca tacttctcca
     4441 gttcgcgttc ctgcaacccc catcgcaccg cctgctgcac ccgctcctca accccgttgg
     4501 ggagcggagg gccaggttga cccgcgaaaa ggtcgtgcgc cacttcatag ggagagtccg
     4561 atgccagctc gatgagcctc gatatccacc ggtccttcac ggggatcaac cgttctgttc
     4621 cggggcggga acgacggccc tgattcatcg ctgaaccgtt tgcatcgtct ctagacttcc
     4681 tgactctgcc agcggccctg gtcatagcgc ggcctcctct ctcaccttcg agatgccccg
     4741 gtactctact cgagccgaca cgggcccacg tccgcgcgcc gcgcatcccc cgcgtgggag
     4801 gagtctgcag cggcaacctg ggctactgcc ggcttccacg tcacgttcca ccgtctcggt
     4861 ctcctatagt tcggcgaacg ccttgcggcg cccccggcgt cgcggcgctt cctcctgcgg
     4921 gaggggctcc gcccggtcta gccactcggc gagaaccagg ttctgctgcg caagtcgctt
     4981 gaagcaagcg atcgcgcggc tgagcagaac gcgtaggtgg tcgcgggtaa ttcccatcag
     5041 aatcgcgatc tcctcgcggg tcagatccgt ttgctccagc tgcagtacct ccgcgtagcg
     5101 cttcggcatg ctcgtgagca cctcctgcac tgctgcgatg atcttcgact cctccgggcc
     5161 ctcgccctcg tcttgatgcg acgcatactg ccaagcccgt gtctcggggt aactgtcata
     5221 gtacacggtg ggcccccact ccgaccggag caggtcatcc gggggcagct cgccggcgat
     5281 caccccgccc cagcgccggt agaagtccgc ccggcagctc tgcgcaactt cgtaaagcca
     5341 gttcttgaag gatccgcgtg cggggtcgta ggtgtggatc gatgcgacga ccttttggaa
     5401 caccatcgag agcacatcgg aggcgtcctc atcgcaggag caccgacgca gaaggtaacg
     5461 gtacaggggc gcggagtagg tgtcgatgag ctgttgcgcc gcgagattat cgccctcgcg
     5521 caggccctgc accagaaaat catcggtccc ttcgtactgc acctggatcc atccttctgc
     5581 tggcatcatg gcgtgtcacc cggcggggca acatccggac gtccgtcgca tttacctacg
     5641 cgagagcgtt acatcccggc gaagcacacg acgtctctga tacgcagcgc cgttacaccc
     5701 cttcgtgaaa cggcacaacc gagagagaac aagggcggct cggagagagg gaaggagacg
     5761 cacatcctgc aggcgcctcc caaaggagcg gctgaccacc cgtgctgcct ataagtatga
     5821 gacgcaagac cgccttgcaa agtttcgatt ttcattcctc tgcttgcatc gcacgcgcgg
     5881 atacggtaga atagcagggg cctgctccca caacaaccga ccgctggccc cccatgcata
     5941 tggggtgaga ggggattgcc gtgacggatc tcggaggcgt attcccggcg ctattgacgc
     6001 cattcgaccg gcaggggcag ttgaacgaac cgatggtgga ggcgctcatc caccacctcc
     6061 tcgaagcggg agtagacggt ttctacgtcg gcggctcgac aggcgagggc cttctgatgt
     6121 ccccccagga gcgccgacgg ctcaccacag cggcagtgcg tgccgcgaac ggcaaggcaa
     6181 aggtgatcgt ccacgtcggc gccaacgcga ccgacgatgc tgtcgacctg gcgcggcatg
     6241 ccgccgaggc cggcgcggac gcagtgagca cgttgccgcc actcttcttc aaggtcggcg
     6301 acgacgccgg cggcaacgcc ccccgccgtg tgaaagacat cgatcacctg cgccagcaag
     6361 g
//
LOCUS       DAOSCV010000114         6357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7800_length_6357_cov_3.207712, whole genome shotgun sequence.
ACCESSION   DAOSCV010000114 DAOSCV010000000
VERSION     DAOSCV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7800_length_6357_cov_3.207712"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..459)
                     /locus_tag="PLY56_04190"
     CDS             complement(76..459)
                     /locus_tag="PLY56_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ20710.1"
                     /translation="MDEKRCRAGFTLVELLIVIAIIAVLASVAIRPLLRAKSAAKESA
                     AIQVIRSLQKAETAYFLRHKRYGNLDDLAGFGGSPVSEGQREAASEYVFKSVADQKGY
                     TIIATPPQANMRTYTLSESGVIEGE"
     gene            904..2910
                     /locus_tag="PLY56_04195"
     CDS             904..2910
                     /locus_tag="PLY56_04195"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ20711.1"
                     /translation="MTSSKTPLWARLARRVPALLLALLALGTAGPIQAGSTFERGLGR
                     PVAEQLEEEVGVIRDPALSGWVERVGSEIIRADGGEPRDYSFKILDSDDVNAFAIPGG
                     FIYVTLGLLCFIDSEDELAAVLGHEIAHLTAHHGVKQLQREILLTLGFVLLRGYVSDM
                     VSTLSQVGGVLYSLRHSRADEAAADAGGLVKMLRAGYDPIAMATFLEKCAEREKQKPS
                     RLEVYFMTHPPTTDRLERATHRPETDPRNTEVRRAVAKGYTERGLYRQAIQAHEQVLA
                     REPHDVAQHLAAARCWDQLGRPDRAHEEWSRAAQLDPDVKLPPTTARKPGPPAIPRAD
                     REAASRARASLADARRQIAAGNNARATQIGDLRKALGNSKRRFRHFLNTLGSAVQPAA
                     TGDLIHQEMLREIGLAVANLDRALSRVQVTLEAAGTVPDRFSESLQALDSQLASSAPD
                     EHLLAIAESLAPSATRSLSELDGGVKKCREALKQTDAAIRVCHAAAQDLQESVFLPAP
                     VVLARVGHLNSRLRVAAKAASSAAALAGEARTLAEAAAARHYILALDRLALSVPAAQK
                     APVCALLTRRYGIPPAQATGLAREGVRLGEATASLLLGLAVRVSLKKDASAEGDAGAI
                     DDRPVKPLSLDEALERRVPLAPLNAGLRLFMLDMERETEGSGQH"
     gene            2907..4862
                     /locus_tag="PLY56_04200"
     CDS             2907..4862
                     /locus_tag="PLY56_04200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ20712.1"
                     /translation="MSALPFPAAGNRPGRTWRLLRAVVTSLLAGLLLATGTPASAGLE
                     TALEKLIGRQAAVALAEQFGTESDPLLADWVKRVGDRVSAVSPRKDVRYRIQVIEMDE
                     PNALALPGGYIFVTKGLLEFIHDDDELASIIAHEVGHVVGRHGIRHIEHQILAALLIS
                     QIRDRGGEMAGLGATLLDALLALQRSRKDELAADRMSVEYAARAGYDPAGLLSFFNKI
                     RRPDKPSWLDTLFATHPPAEKRIEHAQKSSYFHSPSRETLIAIGDRLASEGRYNKALE
                     KYREAAKQGADDAELNEKIALALAAQGRQSETMALQASGGSPLAPAHSPEAPRRAFEE
                     AMPAHQAVLAVHRDLSERQRLLVESGQRTQKRLRNASQRHEWTRRLEAAMLGMPQGLD
                     TRWIYVAARCLALSTEVDSLLRSGWRGVRQANAAMHRATAVCAQAASETDPARYLSRD
                     LLSSIALDMERAGKESIESLARIDRAIPDLAEADRIVAGVMADLNSPYVVPWANPWGH
                     LAALEGFLQHASSCLRRARGHVEAGLRYATRARARSERAALDIADAAASPAEHAIFRT
                     LVRTRLGVTDTAIRDLVGRGHSLGDATVMLLYTRSAGESVSGLEASHQAGASWVETAE
                     RMGLSPETQAIVLRLLNNTIAEEKIPG"
     gene            4906..>6357
                     /locus_tag="PLY56_04205"
     CDS             4906..>6357
                     /locus_tag="PLY56_04205"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOJ20713.1"
                     /translation="MSQVRVVSIGIILLSLLAAGAITARRVAVERANRSVEIVVEWDE
                     IRELALATRGDSRAATPAALLRHLRETHQVRGLAVSEMTLAELRDVGEVFLSHELPVG
                     FADSTPARSGAAPLYLIIPDARLADQILRHVAERRSSAYLTPVPSSQARGSGPVVLRL
                     NTRYDLIKSATIGFPTEAIQAAAEAGVEVIARFGGFTGLTEGSVDAITAYLRELGIQN
                     VIFSGTEVLGNKKLIPYVAESLLRHQIRYGFLEFGKQQGDAALARLMRGQLIRVHSIT
                     GAEMAQITPADATERFVKAARERNIRLLYLRFFTRPADNLVDENGRYLSAIGRNLHRV
                     GLTIGQASPFSALYAGRPLLILTGAGVIAGGVLLLALALGLAARTQWLLLILGISGSS
                     LLLAIDPTLTLARKALALLAALVFPTLAFLRWGNPKPSGGREPAGQTRPDLSVSPLRA
                     LGPMLAMTATTLAGALLIVGLLGDTRFLLKYDQF"
BASE COUNT         1025 a         2223 c         2036 g         1073 t
ORIGIN      
        1 ccggaaagca gcgcggagct tcgagctccg cgcgtgcgcg cggccttctc cttatctcgg
       61 aatctcagct gcgggctact cgccctcgat cacgcccgac tcagagagag tataggttcg
      121 catgttcgcc tggggcggcg tcgcaatgat ggtatagcct ttctgatccg ccaccgattt
      181 gaagacgtat tcgctggcgg cctctcgctg gccctccgag acaggactgc cgccgaaacc
      241 cgccaggtca tccaggttgc cataccgctt gtggcgcagg aagtaggcgg tctctgcctt
      301 ctggaggctg cggatcacct gaatggcagc gctctccttg gcggcgctct ttgcacgcaa
      361 taaaggccgt atcgccacac tcgcgagcac tgcgataatg gcaatgacaa tcagcagctc
      421 gaccagggta aagcccgctc tgcatctttt ctcatccact gtctgttcat ctccctgcgc
      481 gtgcggtgcc gcgctgatac cacctacctg cacctgctat ggcgcggatt cacgacgccc
      541 gaagcttgct ccttctcaca ggccgcgccg attgcgcgtg cctaatcact acccaggggc
      601 tcgttccccc aaacggatct ggagccccgt cttgcatcgc gttgacttct cgcgaaaggg
      661 cgtgctataa tcgcctcaga tggagcagtg ccccatcccg ggcaggctat ggatcgcctg
      721 ctccgcgggc acctggcagg tcgcgcgccg gggttctggc agggttttcc ctcgcccatc
      781 ctccctctat gaagccagag gggctggcga aacgaggcac acgctgctgg atccccgtcg
      841 aaggagaacg ccgccgtatc cgccggccga cgagcgtgcc gcggtcgccg gggaaggact
      901 tgcatgacgt cttcgaagac tcccctttgg gcccgcctgg caaggcgagt gccggccctc
      961 ctgctcgctc tcctcgccct aggaactgcg ggcccgatcc aggccggctc taccttcgag
     1021 cgaggcctgg ggaggccggt cgccgagcag ctcgaagagg aagtaggggt catccgagac
     1081 ccggccctct ccggttgggt ggagcgcgtc ggcagcgaga tcatccgcgc ggatggcggc
     1141 gaaccccgcg attactcctt caagatcctc gatagtgacg acgtcaacgc cttcgccatc
     1201 cccgggggct ttatctatgt caccctcgga ctcctctgct tcatcgactc cgaggatgaa
     1261 ctcgcggcgg tcctcggaca cgagatcgcc cacctcaccg cacaccatgg cgtgaagcag
     1321 ctccaacgcg agatcctgct cactcttggc tttgtcctgc ttcgcggtta tgtctccgac
     1381 atggtctcta ccctgagcca ggttgggggc gtcctctact ccctacggca ttcgcgcgcg
     1441 gatgaggccg ccgctgatgc cggaggactc gtgaagatgc tgcgcgccgg ctacgacccg
     1501 atcgcgatgg cgactttcct cgagaagtgc gccgaacgcg agaagcagaa gccatcccgg
     1561 ctcgaagtgt acttcatgac gcatccccca acaaccgacc gcctggaaag ggccacccac
     1621 cggccagaga ccgatccgcg caacacggag gtccggcgcg cggtcgccaa aggctacacg
     1681 gagcgtggcc tctaccgcca ggcgatccag gcgcacgagc aggtgctcgc gcgtgagccg
     1741 cacgatgtgg cgcagcatct ggcggccgcg cgttgttggg accaactggg ccgcccggat
     1801 cgagcacacg aagagtggtc gcgcgcggca caactcgacc cggacgtgaa gctgccgccg
     1861 accacagcgc gcaagcccgg cccgccggcg ataccacgcg cagatcgcga ggcggcctcg
     1921 cgggcgcgcg cgtctctcgc cgacgccagg cgccagatcg ccgcgggcaa caacgcgcgc
     1981 gccacgcaaa tcggggatct ccggaaggcg ctcggcaaca gcaagaggcg cttccggcac
     2041 ttcctcaaca cgctcggtag cgccgtgcag cccgccgcca ccggtgacct gatccaccag
     2101 gagatgctgc gcgagatcgg cctggccgtc gccaacctcg accgggcgct cagccgcgtg
     2161 caggtgactc tcgaagccgc gggtacggtt ccagatcgct tctccgaaag cctccaggcg
     2221 ctggacagcc agcttgcttc ctcggcccct gacgagcacc tgctggcaat cgccgaatcg
     2281 ctcgcgccgt cggccacccg ctccctgtcg gaactggacg gcggggtgaa gaagtgccgt
     2341 gaggcgctga aacagacaga tgccgccatc cgcgtctgcc acgccgccgc gcaggacctc
     2401 caggagagcg tcttccttcc ggcgcccgtg gtcctggccc gcgtgggcca cctgaactca
     2461 cgcctgcgcg tcgccgcgaa ggcggcatcc agcgccgcgg ctctcgcggg cgaagcccga
     2521 acgctcgcag aggcagcggc ggcacgccat tacatcctcg ccctggatcg gctggccctt
     2581 tctgttccgg cggcccagaa agcccccgtg tgcgctctgc tcacccgccg ctacgggatc
     2641 ccgccggcgc aggccaccgg gcttgcgcgc gagggggtgc gcctggggga ggccaccgct
     2701 tcacttctcc tcggcctggc cgtccgcgtt tctctgaaga aggatgcttc cgcggagggc
     2761 gatgccggcg ccatcgatga ccggcccgtc aagcccctct ccctcgatga ggccctggag
     2821 cgccgcgtgc ccctggcgcc gttgaatgca ggcctacgcc tcttcatgct cgatatggaa
     2881 agagaaacag aggggagcgg acagcattga gcgctcttcc cttccccgcc gccggcaacc
     2941 gtccagggcg aacctggcgc ttgttgcgcg cggtggttac ctctctcctg gcggggctcc
     3001 ttctggctac gggcactccc gcatccgccg gactcgaaac cgctctcgag aagctgatcg
     3061 ggcggcaggc agcggtcgcc ctggccgagc agttcggcac ggagagcgac ccgctgctgg
     3121 cggactgggt gaagcgggta ggtgaccgcg tgagcgccgt cagcccgcgc aaggatgtgc
     3181 gctaccggat ccaggtgatc gagatggacg agccgaacgc cctggcgctg ccgggcggct
     3241 acatcttcgt gaccaagggg ctgttggagt ttatccacga tgatgatgag ctcgcctcga
     3301 tcatcgcgca cgaggtgggg catgtggtcg gcagacatgg gattcggcac atcgagcacc
     3361 agattctcgc ggcgctcctc atctcgcaga ttcgggatcg gggcggagag atggcgggcc
     3421 tgggggccac tctcctggat gccctgctgg cgcttcagcg aagccggaag gatgagctcg
     3481 cggcggaccg gatgagcgtg gagtatgccg cgcgcgccgg gtatgatccg gccggcctgc
     3541 tcagcttctt caacaagatc cgccggccag acaagccatc ctggctcgat acgctcttcg
     3601 ccacgcaccc cccggcggag aaacggatcg agcacgccca aaagagttcc tacttccact
     3661 ccccctcccg cgagacgctc atcgctatcg gcgaccggct cgcttccgag ggccgttaca
     3721 acaaggcgct ggagaaatac cgcgaggctg ccaaacaggg cgctgatgac gccgaactga
     3781 acgaaaagat cgctctggca ctcgccgcgc agggcaggca atccgaaacg atggcactgc
     3841 aggcgtccgg gggctccccg cttgcgccgg cccactctcc cgaggcgccg cggcgggcct
     3901 ttgaggaggc gatgcccgcg catcaggcgg tcctcgcagt gcatcgtgac ctctccgaac
     3961 gccagcgcct gctggtggaa agcgggcaac gaacccagaa gcggctacgg aacgcatctc
     4021 agcgccacga gtggacgcgg cgcctggagg ccgcgatgct cgggatgcct caagggctgg
     4081 atacccgctg gatctatgtg gccgcgcgct gcctcgcgct ctcaaccgag gtcgactcgc
     4141 tccttcgaag cgggtggcga ggagtgcgcc aggcgaacgc cgccatgcac cgggcgaccg
     4201 ccgtgtgcgc ccaggcggcc tcggaaacgg acccggcccg atacctctcg cgcgatctcc
     4261 tctccagcat cgccctcgat atggagcgcg ccggaaagga gagcatcgag agcctggcgc
     4321 ggatcgaccg cgcgattccc gatctggcgg aggccgaccg gatcgtggcc ggcgtcatgg
     4381 ccgatctcaa tagcccctat gttgttccgt gggccaatcc ctgggggcat ctggccgcgc
     4441 tggagggttt cctgcaacac gccagctcct gtttgcgccg cgcacgcggg cacgtggagg
     4501 ccggcctgcg atacgccacc cgcgcgcgtg cgcgcagcga gcgcgctgcc ctcgatatcg
     4561 ctgacgcggc ggcgagcccc gcggaacatg ccatcttccg cacgctggtg cgcacccggc
     4621 tcggggtcac cgacaccgcg atccgcgacc tggtgggccg cgggcattcc cttggcgatg
     4681 ccacggtgat gctactctat acccggagtg ccggcgagtc cgtatccggc cttgaagcct
     4741 cccaccaggc tggcgcatcc tgggttgaaa ctgcggaaag aatggggctc tctccagaga
     4801 cgcaggcaat cgtgctccgt ttgttgaaca atacgattgc tgaggaaaag atccccggct
     4861 gatgcttggt ccatcgttct attcaccttt gctgaggagt gttacgtgtc ccaggtccgt
     4921 gtggtctcta tcggaatcat attactctca ttgctggccg caggcgcgat cacagcgcga
     4981 cgcgtggcgg tagagcgcgc caaccggagc gtggagattg tggtggagtg ggacgagatc
     5041 cgcgagctcg ccctggcaac ccgcggcgac tcccgagccg ccacccccgc ggcgctgctc
     5101 cgccacctgc gggagaccca ccaggtccgc ggcctggccg tgagcgagat gactctcgcc
     5161 gagttgcgcg atgttggcga agtgtttctc tcccacgagc tccctgttgg ctttgcggat
     5221 tcgacgccgg cgagatccgg ggcggcgcca ctctacctga ttatcccgga cgcccgcctg
     5281 gccgatcaga ttctccggca tgtggccgag aggcggtcgt ccgcctacct gacgcccgtg
     5341 ccgtccagcc aggcccgcgg ctcaggcccg gtggttctcc gcctgaacac gcggtatgac
     5401 ctgatcaagt cggccactat cggcttcccg acagaggcca tccaggcggc ggccgaggcc
     5461 ggggtggagg tgatcgcccg ctttggcggc ttcacagggc tcacggaggg gtcggtggat
     5521 gcgatcaccg cctacctgcg ggagcttggc atccaaaacg tcatcttctc cggcacggaa
     5581 gtgttgggca acaagaagct catcccctac gtggccgaat cgctgcttcg ccatcagatc
     5641 cgctatgggt ttcttgagtt cggcaagcag cagggcgatg cggctctggc gcgcctgatg
     5701 cgcggccagt tgatccgcgt gcacagcatc accggggccg agatggcgca gatcacccca
     5761 gcggatgcca cggagcgctt tgtcaaggcc gcccgtgagc ggaatatccg cttgctgtac
     5821 ctgcgcttct tcacgcgacc agccgacaac ctggtggatg agaatggccg gtacctgagc
     5881 gccatcgggc gaaacctgca ccgcgtgggc ctcaccatcg gccaggccag ccccttctcc
     5941 gcgctctacg ccgggcggcc cctgctcatc ctcaccggcg cgggggtcat cgcgggcggc
     6001 gtgcttcttc ttgccctcgc gctcgggctc gccgcgcgta cccagtggct cctgctcatc
     6061 ctcgggatct ccggcagctc cctcctcctt gccatcgacc caacgctcac gctggcccga
     6121 aaggcgctgg cactgctggc cgccctggtc ttccccaccc tcgccttcct ccgctggggc
     6181 aacccgaagc cttccggcgg ccgcgagccc gcgggccaga cgcgcccgga tctgagtgta
     6241 tcgccgctgc gcgctctcgg tccgatgctc gccatgacgg cgacgacgct ggccggggcg
     6301 ctgctcattg tcgggctgct cggcgacacc cggttcctgc tcaagtacga ccagttc
//
LOCUS       DAOSCV010000115         6338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7832_length_6338_cov_3.319911, whole genome shotgun sequence.
ACCESSION   DAOSCV010000115 DAOSCV010000000
VERSION     DAOSCV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7832_length_6338_cov_3.319911"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="PLY56_04210"
     CDS             <1..2469
                     /locus_tag="PLY56_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20714.1"
                     /translation="VLRDGQSALLPAVAGLLRRADLKSAGFSSSRIVGYNGATWTRHL
                     LWQKGSYFVVIDEVTAETPGDYTLGSTWRVLGEPRLEGNALTITQRESSARVQNADGL
                     AGRIRFERMAGAWPIHALVFRVGRRLAQGETHLFRNFFYASSPNRPQSLRALPAGAEG
                     VLVTGGPTPAWWGVSGPDRPARRGKLETDAALFRITPSGYALASARRLVAGDPLFSSD
                     QPVDIGLDAERGEGVLVSEKPAKVVLRLAPGATWTLGREKGRADADGTVRLELALGRH
                     TLKADRFALPAAIAEALAAPSQGAAAAVGAREEPTPAAWTYKEFRAERRPLKVQSVRC
                     DQPHVGRYGPVEKLADGGYRSSLQSVQWARGVAPEVTFDLGEIREIDSVRIRAWELTE
                     AQKGARRELWLSNDGFQTDRRRVDAEFAPAGTEEFGTNINFLYTCAVRQKARYVRLKM
                     TPAGEGSIIYLAEVEFFGGDPDGRAEITALASGRLGPAGELATVASTASGEIVAVDAE
                     GKRLWTYQARGRVYALAVADLDGDGRDEVIAGGQPAWLLCLDATGKRRWEHRPRAYRG
                     LAGDVITLCVGDLEADGKPEVVAGLLCWKYIAVDAEGKEKWDTVIYAHSATACALADV
                     NGDGRRSVIAGNAYYRLNLLGPDGRVSWQNYTLGPEHTCVAAADLDGDGKEEILAGSD
                     LGEFHAITGARERLWEVNAGDAVRAILTHRPKGAPLEVYAAGDSGFVYACKPDGSPRW
                     KVSVGDAINGALLADVDGDGQPEIVAAAGSAGVVILGRDGKIRRSLRAASPVTHVQVL
                     PGKLPRIVAALADGSLAVWPATPR"
     gene            2769..3833
                     /locus_tag="PLY56_04215"
     CDS             2769..3833
                     /locus_tag="PLY56_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20715.1"
                     /translation="MKALSVGILGLGHLHPRSYMPHFEAIDGVSVVAVADANPALLDH
                     FVQEFPVRGYADWREMIDKEKLDLAVLFLPHAQCPDAAVACAERGIHLIVEKPMAADA
                     AGVRRMIDAAEKSGVLLSTAYVWRYHPVACQMKQLVQSGVLGKPVGGEGRCAAGRLDR
                     YIQGNASWMLQRELSGGGPMFNLGVHWIDLYRWMLEDEVAEVIGKNVKVNDQYDIEDN
                     SFALLTFSRGAVIALDISYTVPDSYPYGRDLALSLRGTGGAISWSPSFEGVRETLFVC
                     SDTGEYAASPRRHLSFELESVPGYSGVMGRQYLADIAECIRTGGKPPITGQDGARALE
                     VVEAIYQSAETGRAVRLSAE"
     gene            3880..4866
                     /locus_tag="PLY56_04220"
     CDS             3880..4866
                     /locus_tag="PLY56_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HOJ20716.1"
                     /translation="MSVKVVITDYIEPDLKWEEKELAKRGFPFTYHQLKFAPVEQVIE
                     ATRDADVVVVNMVKITREVIAAWEKCRLVIRHGVGYDNVDVDALTEKGIPLCYIPDYC
                     AEEVAEQAIALIFACARRIPASRKVLEESSARGQWDFTDVIPIYRMAGRTLGILGCGR
                     IGSYVYRKLQSFGFNFLICDPYLSEERRRELGVELVDKETLFRESDFITLHAPLNDET
                     RRIVNAETLAMMKPTAYVINTARGPLVDHQALADALRNGTIAGAAIDVFDKEPPETDY
                     PLFGLPNAILTPHLAWYSEDAAWRIREIILLEIDRFAAGQPPRYCVNPQVLS"
     gene            4922..6247
                     /locus_tag="PLY56_04225"
     CDS             4922..6247
                     /locus_tag="PLY56_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ20717.1"
                     /translation="MSDAKKVACVKGPLPGPRSRELFERWRKAEAQCAGYQAQVVWDH
                     ARGVVVTDVDGNTFLDWTSGVLVTNVGHCHPALVQAIQQASEKLLNNYECLNEPRVIA
                     AEKLTSVLPRPLDRCFFLSTGSETVEAAVRIMKRKTGNYEVISFYGGFHGRTYAAAAA
                     GGMPGPKKGYGPTLPGAIRAPYPYCYRCPFKAKPETCGMLCLEFLDDCVRANSTGSLA
                     GLIVEPYQGASGFIFPPEGWLSRLEQWVRSKGLVFTVDEVQASYGRTGKFWAVEWEGV
                     QPDLLCIGKGIGSGLPVSAIAARSEVIGVLGPGEMSSTMGGNPVASACVTAVIDIMLR
                     ENLAENARRMGELLGKRLREIQERSPYVGDVRGKGLVFGVELVKDKATKEPAPDLTRK
                     LIDVAAQNGLLIGSVGVFGNVIRVAPPLVINEAELDESLEIFERSLGQL"
BASE COUNT         1027 a         2003 c         2249 g         1059 t
ORIGIN      
        1 gtgctccgcg atgggcagag cgccctcctc cccgcggtgg ccggattgct acgccgggcc
       61 gacctgaaga gcgccggctt ctcctcttcg cggattgtcg gttacaacgg cgccacctgg
      121 actcgtcacc tcctctggca gaaggggagc tactttgtcg tcatcgatga ggtgacggca
      181 gagaccccgg gcgattacac cctggggagc acctggcgcg tgctgggtga gccgaggctg
      241 gaagggaacg cgctcactat cacacagcgt gagagctcgg cgcgggtgca gaacgctgat
      301 ggcctagccg ggcgcatccg gttcgagcgg atggcggggg catggccgat ccacgcgctc
      361 gtcttccgtg ttgggcgccg cctggcccag ggagagacgc atctcttccg caacttcttc
      421 tacgcgtcgt cgccgaaccg gccgcagtcg ctccgggcgt tgcctgcagg ggcggaggga
      481 gtgctggtaa cgggaggacc cacgccggca tggtggggcg tttcaggccc cgaccggccc
      541 gcccggcgcg gcaagcttga gacggacgcg gcgctcttcc ggatcacccc ctcgggctat
      601 gccctggcct ccgcgcgccg cttggtcgcg ggggaccctc tcttcagcag cgaccagccg
      661 gtcgatatcg ggctggatgc ggagcgcggc gagggcgtgc tggtctcgga gaagcccgcc
      721 aaagtggtgc tccggctggc gcctggcgcg acgtggacgc tgggccgcga gaagggccgg
      781 gcggacgctg acggcaccgt tcgcctggag ctggcgctag gccggcacac gctgaaggcg
      841 gatcgcttcg ccctcccggc cgccatcgcc gaggcgctgg cggcgccatc ccagggagcg
      901 gccgcggcgg ttggcgcgcg tgaggagccg acgccggctg cctggaccta caaggagttt
      961 cgcgcggaac gccgccctct caaggtccag agtgttcgct gtgaccagcc gcatgtgggg
     1021 cgctacggcc cggtggagaa gctggcggat ggcggctacc ggagctcgtt gcagtcggtg
     1081 cagtgggcgc gcggcgtcgc cccagaagtg acgttcgatc tgggagagat ccgcgagatc
     1141 gacagcgttc gcatccgcgc ctgggagctc accgaggcac agaagggcgc gcggcgcgag
     1201 ctctggctga gcaacgacgg tttccagacc gaccgccgcc gcgtggacgc ggagttcgcg
     1261 ccggcgggca ccgaagagtt cggaacaaac atcaacttcc tctacacctg cgcggttcgg
     1321 cagaaggcgc gctacgtgcg cctgaagatg acccccgccg gcgaggggag catcatctac
     1381 ctggcggagg tggagttctt tggtggtgat ccggacggcc gtgcggagat caccgcgctt
     1441 gcgtccgggc ggcttggccc cgcgggagag ctcgccacag tggcctcgac ggcaagcggc
     1501 gagatcgtcg ccgtggacgc cgagggaaag cggctgtgga cgtatcaggc gcgtgggcgc
     1561 gtctatgccc tggccgttgc cgacctggat ggcgacggcc gcgacgaggt gatcgccggg
     1621 gggcagccgg cctggctcct ctgcctggat gccaccggga agcgccgctg ggagcatcga
     1681 cctcgggcgt atcgcggcct cgcgggcgat gtgatcaccc tgtgcgtggg tgatctggaa
     1741 gctgacggca agccggaggt agtcgccggg ctgctgtgct ggaagtatat cgccgtggac
     1801 gcagagggca aggagaagtg ggacactgtc atctatgccc atagcgccac ggcgtgcgcc
     1861 ctggcggacg tgaatggcga tggccggcgc tccgtgatcg cgggcaacgc ctactaccgg
     1921 ctcaacctgc tgggtccgga tggccgggtg agctggcaga actacaccct ggggccggag
     1981 cacacctgcg tcgccgcagc cgacctggat ggcgatggca aagaggagat cctggcgggg
     2041 agcgacctgg gcgagttcca cgcgatcacc ggcgcgcgcg agcgcctgtg ggaggtgaat
     2101 gccggcgatg ccgtgcgcgc cattctgacg caccggccca agggggcgcc cctggaggtg
     2161 tacgccgcgg gcgatagcgg attcgtctat gcgtgcaagc cggatggctc gccgcgctgg
     2221 aaggtctcgg tcggcgatgc gatcaacggc gcgctcctcg cggacgtgga tggcgacggc
     2281 cagccagaga tcgtcgcggc ggccgggagc gccggcgtgg tgatcctcgg gcgcgacggc
     2341 aagatccgcc ggtcgctgcg cgctgcctcg cctgtgacgc acgtgcaggt gctccccgga
     2401 aagctgccgc ggatcgtcgc tgccctggcc gatggttccc tggctgtctg gccggcgact
     2461 cccagatgag gcggatccgg ctcaccagca tctgacgcgc aaccgtccga aaccgggcat
     2521 cagcatctgc gtatcgcggt ggcatgctgg ccgggtgccg ggcgcgtgcc gcgcatcctc
     2581 gggaaggtgg agagaaaagg gcgggagaac cccgcccttt ctctttcccg gagcagcggt
     2641 tttgccgcgc cgcctgctgg ccgcccggag cctccgtacc cctcgcaccg gatgccatcc
     2701 gggtttagca ggaaacgccc gccccggtcg ccaagaaaga gaatgtgaga acggagggct
     2761 cgggccgtat gaaggcgtta tctgtaggta tcctggggct gggccacctg cacccgcgca
     2821 gttacatgcc ccattttgaa gcgatagatg gcgtgagcgt tgtggccgtc gcggacgcca
     2881 accccgctct tctcgatcat ttcgtccaag agttcccagt gcgcggctat gccgactggc
     2941 gcgagatgat cgataaggag aagctggacc tggccgttct cttcctgccg catgcgcaat
     3001 gcccggatgc ggctgtggcc tgtgccgagc gggggatcca cctcatcgtc gagaagccga
     3061 tggccgctga cgcggcgggc gtccggcgga tgatcgatgc cgcggagaaa tcgggcgttt
     3121 tgctgagcac tgcctacgtc tggcgctacc atccggtggc gtgccagatg aagcagctcg
     3181 tccagagtgg cgtgctgggg aagcctgtgg gaggcgaggg ccggtgtgcg gcaggccgcc
     3241 tcgatcgcta tatccagggc aacgcgagct ggatgctgca gcgagagctg agcggtggcg
     3301 gaccgatgtt caacctgggc gtccactgga tcgacctcta tcgctggatg ctcgaggatg
     3361 aggtggccga ggtcatcggc aagaacgtga aggtgaatga ccaatacgac attgaggaca
     3421 actccttcgc gctcctcacc ttttcccgcg gcgcggtgat cgcgctcgac atcagctaca
     3481 ccgtgccgga ttcctatccc tatgggcgcg atctggcgct ctcgctgcgc ggcaccggcg
     3541 gagccatctc ctggagcccc tccttcgagg gggtgcgcga gacgcttttc gtctgcagcg
     3601 acaccggtga atatgcggcc tcgccgcggc ggcacctcag cttcgagctg gagagcgtgc
     3661 cgggctatag cggcgtgatg ggccggcaat acctggccga tatcgctgaa tgcatccgca
     3721 ccggcgggaa gccacccatc accgggcagg atggcgcgcg cgctttggaa gtggtggaag
     3781 cgatctacca gtcggccgaa actggccgtg ccgtgcggct ttccgcggag tgagcggcgc
     3841 ctggggcccg tcatcgcatc cgccaggatc ggagtttcga tgagcgtgaa ggttgtcatt
     3901 acggactata tcgagccgga cctgaagtgg gaggagaagg agctggccaa gcgcggcttc
     3961 cccttcacct accaccagct caagttcgcg ccggtcgaac aggtgatcga ggccacgcgc
     4021 gatgccgacg tggtcgtcgt gaacatggtg aagatcacgc gcgaggtgat cgccgcgtgg
     4081 gagaagtgtc ggctcgtgat ccggcacggt gtcggctacg acaatgtgga tgtggacgcg
     4141 ctgacggaga agggaatccc cctctgctat attccggact actgcgcaga ggaggtggcc
     4201 gagcaggcga tcgctctgat ctttgcctgc gcgcgccgga tccccgccag ccgcaaggtg
     4261 ctggaagaat cctcggcgcg tggccagtgg gacttcaccg atgtaatccc gatctaccgc
     4321 atggcggggc gcacgttggg catccttggg tgcgggcgca tcggcagcta cgtctaccga
     4381 aagctgcagt cgttcgggtt caacttcctc atctgcgacc cctacctgag cgaagagcgc
     4441 cgccgcgagc tgggcgtcga gctggtcgat aaggagacgc tcttccgcga gtcggacttc
     4501 atcacgctcc acgcgcccct caatgatgag acgcggcgga tcgtcaacgc cgagaccctg
     4561 gcgatgatga agccgacggc gtatgtaatc aacaccgcac gcgggccgtt ggtggaccat
     4621 caggcgctgg cggacgcgct gcgcaacggc accatcgctg gcgcggcgat tgatgtcttt
     4681 gataaggaac cgccggagac ggactatccc ctcttcggct tgccgaatgc catcctcacc
     4741 ccgcacctgg cgtggtactc ggaggatgcc gcctggcgta tccgcgagat catcctcctg
     4801 gagatcgacc gctttgccgc cggccagccg ccgcgctact gcgtgaatcc ccaggtgttg
     4861 agctgagggc acgagggcga cctgtgggcc cgccctcacg ggatcgatgg gaacgtgatt
     4921 catgagcgat gcgaagaaag tggcgtgcgt gaagggcccc ttaccgggac cacgctcgcg
     4981 agagttgttt gaacgctggc gcaaggcaga agcgcaatgt gccggctatc aggcgcaggt
     5041 ggtgtgggac cacgcgcgcg gcgtcgtggt gaccgacgtc gatggcaaca ccttcctcga
     5101 ttggacctcc ggcgtgctcg tcaccaacgt tggacattgc caccctgctt tggtccaggc
     5161 gatccagcag gcgtccgaga agctgctcaa caattacgag tgcctgaatg agccccgggt
     5221 gatcgccgcc gagaaactca cctctgttct gccacggccc ctcgataggt gcttcttcct
     5281 cagcaccgga agtgaaaccg tggaggccgc cgtgcgcatc atgaagcgca agaccggcaa
     5341 ctacgaggtg atcagcttct atgggggctt ccatggccgc acctatgccg cggccgcggc
     5401 cggcggaatg cccggtccca agaaggggta tggccccacc ctccccgggg cgatccgcgc
     5461 gccctatccc tactgctacc gctgcccctt caaggcgaag ccggagacgt gcgggatgct
     5521 ctgcctggag ttcctggacg attgcgtgcg cgccaacagc accggcagcc tggcgggctt
     5581 gattgtcgag ccgtatcagg gcgcctcggg cttcatcttc ccgcccgagg gctggctctc
     5641 ccgcctggag cagtgggttc gctccaaggg gctggtcttc accgtggatg aggtgcaggc
     5701 ctcctatggg cgcaccggca agttctgggc cgtggagtgg gagggcgtgc agcctgacct
     5761 cctctgcatc ggcaagggaa tcggcagcgg gcttcccgtc tcggcgattg ccgcgcgcag
     5821 cgaggtgatt ggtgtcctcg gccccggcga gatgagcagc acgatgggcg gcaaccccgt
     5881 cgcttccgcc tgcgtgactg cggtgatcga tatcatgctc cgcgagaacc tggcggagaa
     5941 cgcgcggcgc atgggcgagc tgctcggcaa gcgcctccgc gagatccagg agcgctctcc
     6001 ctacgtgggc gacgtgcgcg gcaagggcct tgtcttcggc gtcgagctgg tgaaggacaa
     6061 ggcaacgaag gaaccggcac cggatctgac gcgcaagctg atcgacgtgg ccgcgcagaa
     6121 cggcctcctg atcggatccg tcggcgtctt cgggaacgtg atccgcgtgg ccccgcccct
     6181 cgtgatcaac gaagccgagc tggacgagag cctcgagatc ttcgagcgct cgctggggca
     6241 actgtagcgg cgtgtctgaa gagggcgggg agaccggctg gcccgctcgg gccgccgacg
     6301 cctcctacac acactcttcg agggagcgcc gcttttct
//
LOCUS       DAOSCV010000116         6333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7836_length_6333_cov_3.797388, whole genome shotgun sequence.
ACCESSION   DAOSCV010000116 DAOSCV010000000
VERSION     DAOSCV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7836_length_6333_cov_3.797388"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1860)
                     /locus_tag="PLY56_04230"
     CDS             complement(<1..1860)
                     /locus_tag="PLY56_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20718.1"
                     /translation="MARADAEAGTVPMGLWRASGGTWLALVHSPAGVWLWSSEGATSS
                     GRLSRRIPLVGAEGVAPLATPGPVVAAACSLDASDRVHLAWQAGETLYYARCALEDLP
                     KGTGWRGPMGEPAPVVVARGVTPRACALAAGARPAIAGERGGGVVAYRFDGEWRETFA
                     SIEGRAPVMLRGNRRELHLAFASPEGIRYHSSPDGERWLPRETPLRGSVGSRPSLALL
                     QGRPVVAGVVNGRAAIATRGEEWSSHSLWDVEEGGHPHLGTDRQGGVWCAWTDGGRIH
                     AARWLGEALSPEFALGAGTAVLLVEQAPPDLAADLGLLVAGAGQPIILRLPTPGLALL
                     ENTPTRFLDLLDLVEMRGVVRDVPALASAGSIPAPAPGALIPGGLVASGKGDRNLRAW
                     FTLQAEGGRIGYVAEWSRGRWGTPLELTLAAPRPWLKPPAGHLSVVRDDEEPNAARRY
                     KMAAESAGSAPALLTSPDGIAWAYYSELPLARPLSLVRDTFARAEERWRLFGLHGEQG
                     SLLHYSSPDREHWYRQPDVGLATADATFWTEEGMLLGLVNGGRLLLAKRRGCPIIQVA
                     EFPMPPGTGRVFAPPFYWNGKRSLFLAEPDVWSRFLAPEGRWVGLAAAAPDRPG"
     gene            complement(1980..3248)
                     /locus_tag="PLY56_04235"
     CDS             complement(1980..3248)
                     /locus_tag="PLY56_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20719.1"
                     /translation="MEQGKRVKDFRKLYQAGQALVRRHKYDQAIAKFKRITGSDLHTL
                     PLPDAGGETTVVEGAESDAAALRPVYVQAHVSLAVCYTEKGMFQEAISILNRAAQIDP
                     QNADALCALGYVYRRNGMVDQAIEAFEKALACNPRCARAQKSLAFLAMEQGRYDDAIL
                     LCQEAIRADPTYEQAYVELAGALQLAGRHAEALEALQKAVSLNPKNLEYLLRAISLLR
                     EAGLQEEALPGLEWAASVCDEPAIRLALGECYLDLDRNREAMEACCAVLRKRPRDLRA
                     LDLLQTAYLKEGETAQALRIAYRLAAICPQDPMNFFRKAVLYQQLGDVRQAIDNYTRV
                     IEMASENDPDLAARAREAVELLDSLQLRQMITLAADDPVFRARLQRDPVEAAAARGFH
                     LSESGLAALQQIELEQLPSLQLQWLRRNYH"
     gene            3929..4603
                     /gene="nfi"
                     /locus_tag="PLY56_04240"
     CDS             3929..4603
                     /gene="nfi"
                     /locus_tag="PLY56_04240"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011317674.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOJ20720.1"
                     /translation="MDRGREHWMVDVREAIALQQELRKRIRLDDDLDEVRTVAGVDTA
                     FPKRGGEPVARCAVVVLSFPELRPVAQVTSVVPITFPYVPGLLAFREGPAIEAALSQL
                     QRLPDLLLFDGHGYAHPRRMGIATHLGVLLDRPTLGCAKSILTGHAEEPGPNPGDRSP
                     LLAPDGELLGYAVRTRRGVKPVYVSAGHRMRHETAVELVLRCARGYRLPEPTRLADKL
                     AGTEMA"
     gene            complement(4776..5939)
                     /locus_tag="PLY56_04245"
     CDS             complement(4776..5939)
                     /locus_tag="PLY56_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010272774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20721.1"
                     /translation="MSSVRVGIIGYGGMGSFHANYLFKGEVPDATLTAVCDVAPERLE
                     AAKKAFGDQVKTFETADALFAAGVVDAVLIATPHYFHPPLAIQAFQHGCHVLSEKPAG
                     VYTKQVREMNEAAERSGKVFGLMFNQRTLAGHQKMRELVQSGELGEIRRTHYVITDWF
                     RTQAYYNSGGWRATWSGEGGGVLANQCPHNLDLWQWICGMPSRVRAFCSFGKYHDIEV
                     EDDVTAYVEYPNGATGVFITSTGEAPGSNSFEITGDRGRLVLEGGKLTFWRTRSSVQK
                     YIYEAQGGFQKPETWQIEIPAGGGEGHKGITKNWIQAITSGAPLLARGEEGINGVQLA
                     NAMLLSTWTDDWVEIPVDEELFLARLKEKIETSTVQKSSGSALNFDGTFGSGA"
BASE COUNT         1063 a         2226 c         2023 g         1021 t
ORIGIN      
        1 cccaggccgg tcaggagccg ccgcggccag cccaacccag cgcccctccg gcgcgaggaa
       61 ccgactccaa acgtccggtt ccgcgaggaa gaggcttcgc ttcccattcc agtagaatgg
      121 cggcgcgaaa accctgcctg tgccaggagg cattgggaac tctgccactt ggatgatcgg
      181 gcacccgcgc ctcttggcca gaagcaagcg tccgccgttc accaggccga gcagcatccc
      241 ctcctccgtc cagaaggtgg catccgcggt cgccagaccg acgtcaggct gccgatacca
      301 atgctcgcgg tcggggctgc tgtagtggag gagtgatccc tgctccccgt gcaaaccgaa
      361 gagccgccac cgctcttccg cgcgcgcgaa ggtgtcgcgc accaagctga gcgggcgcgc
      421 caggggaagc tcagaatagt aggcccaggc gattccatcc ggcgaggtga ggagcgcggg
      481 ggccgatccg gcgctctcag cggccatctt atagcggcgc gccgcattcg gttcctcgtc
      541 atcccgaacc acggagaggt ggcccgccgg gggtttcagc cacgggcgcg gtgccgcgag
      601 cgtcagttcc agcggggttc cccaccggcc ccgagaccac tcggccacat acccgatccg
      661 gcccccctct gcctggagag tgaaccaggc gcgcaggttg cgatccccct tgccagaggc
      721 taccagcccc cccggaataa gcgcgccggg ggccggtgcg gggatcgacc cggcactcgc
      781 gagcgccggc acatcgcgaa caacgccccg catctccacg agatcgagca ggtcaaggaa
      841 gcgcgtgggc gtgttctcca ggagggcgag tcccggcgtg ggaaggcgca ggatgatcgg
      901 ttgccctgcc ccggccacca ggagaccgag atccgcagcc agatccggcg gcgcctgctc
      961 cacgaggagc acggcggtgc ctgcgccgag cgcgaactct gggctcaggg cctcgcccag
     1021 ccagcgggca gcatggatcc gaccgccatc cgtccaggca caccacactc caccctggcg
     1081 gtctgttccc agatggggat ggccgccttc ctccacatcc caaagggaat gagatgacca
     1141 ctcctcgccc cgggtggcaa tcgcggccct gccattgacc acgccggcga cgacgggccg
     1201 gccctgcagg agcgccagcg atggtctgga tccgacgctc ccgcggaggg gcgtctcgcg
     1261 cggcagccac cgctcgccat cagggctgga gtgatagcgg atcccctctg ggctcgcgaa
     1321 ggcgaggtgc agttcgcgcc ggttgccgcg cagcatcacc ggggcacgcc cctcgatact
     1381 ggcgaacgtc tcgcgccatt ccccatcaaa ccggtaggca acaacgcccc cgccacgctc
     1441 tcccgcgatg gccgggcgcg caccggcagc gagggcacac gcgcgcggcg tcactccgcg
     1501 cgccacaacc acgggcgccg gctctcccat cgggccgcgc cagcccgtgc ctttggggag
     1561 atcctccagg gcgcagcgcg cgtagtagag cgtttcaccc gcctgccagg ctagatgcac
     1621 gcggtccgag gcatcgagag agcaggcggc ggccacgacg gggccgggcg tcgcgagggg
     1681 tgcgaccccc tccgctccca ccagcgggat gcgccgggaa agccggccgg aagaggtggc
     1741 tccctcggag gaccagagcc agacgccggc aggagaatgg acgagcgcga gccaggtgcc
     1801 gccagaggcg cgccaaagtc ccatgggcac ggtgccggcc tccgcgtcgg cgcgcgccac
     1861 tgaggatcct cccgcgatga ggcagagaag caggctgatg agcgctatgc caagcaggcg
     1921 ccggccacgg tcacggcggg atgtccctgt tgaggcgaac cggtcggtag gcaaggccgt
     1981 cagtggtaat tccggcgcag ccactgaagc tggagagagg ggagctgttc cagctctatc
     2041 tgctgcagcg cagccaggcc ggactcgctg agatggaagc cgcgcgccgc ggcggcctcg
     2101 acgggatcgc gctgcagccg ggcgcggaag acaggatcgt cggcagccag ggtgatcatt
     2161 tgccgcagtt gaaggctatc cagcaactcc accgcctctc tcgcgcgcgc cgccagatcc
     2221 gggtcattct ccgaggccat ctcgatcacg cgcgtgtagt tatcaatcgc ctggcgaaca
     2281 tcgccaagct gctgatagag cacagccttg cggaagaagt tcatcgggtc ttgcgggcag
     2341 atcgcggcca gccggtaggc aatccgcagc gcctgggcgg tctctccttc cttgagatac
     2401 gccgtctgca agagatcgag cgcgcgcagg tcgcgcgggc gcttgcgaag cacggcgcag
     2461 caggcttcca tcgcctcgcg gttgcgatcc agatcgagat agcactcgcc cagagccaat
     2521 cgaatcgccg gctcgtcgca gacgctcgcc gcccactcca ggccaggcaa cgcctcctcc
     2581 tgcaagcccg cttcacgcag cagcgagatg gcgcgcagga gatactccag gttcttcggg
     2641 ttgagcgaga ccgccttctg gagggcttcc agcgcctcgg catgccggcc ggccagctgg
     2701 agcgccccgg ccagctcgac ataggcctgt tcgtacgtcg ggtcggcgcg aatcgcctcc
     2761 tggcagagga ggatggcgtc gtcataccgc ccctgctcca tcgccaggaa cgccaggctc
     2821 ttctgagcgc gggcgcagcg cgggttgcac gccagcgcct tttcaaacgc ctcaatcgcc
     2881 tggtcgacca tgccgttgcg ccgatagacg tagcccaacg cgcagagcgc atccgcgttc
     2941 tgggggtcaa tctgcgcggc gcggttgaga atgctgatcg cctcctggaa cattcccttc
     3001 tcggtgtagc agacagcaag gctcacatgc gcctgcacat acaccggacg cagcgccgcg
     3061 gcgtcgctct ccgcgccttc gaccaccgtg gtctctcccc ccgcatcggg aaggggcagc
     3121 gtgtgcaggt cactcccggt gatccgcttg aacttggcga tcgcctggtc gtacttgtgc
     3181 cggcgtacca gtgcctgacc cgcctggtac agctttcgga aatcctttac ccgttttccc
     3241 tgttccattg agggaccgcc tcccggtccc ggccgaaacc cactctgctc ctcgttcgac
     3301 cagttccctg tgcattcgat tcgacagggg gccgcgcgcg tcctcccggc gcttcctgct
     3361 cctttttgga ggccgcaccg cgcctggagg ctccatcaga tgttgcgcgt gtattccagc
     3421 gattgggatt ccggaatggg taaggtccgg acggttgccc gcgttctccc gcccgcgccc
     3481 ggccccgata ccacggatga agggaacgga cacggatggc aagggctgcc ctcgcgccag
     3541 gagctcgaaa ctccgcgctg cttcccggca aagccccgtc ggggctcccc cctcccagat
     3601 cttgccactt ccgcttggcc gggaatgtgg tccgacccag cccggcaggg cttcgctcgc
     3661 aagcagcgcg gtgatttatc gccgcgcgtc gggttgcgcg acaccctggc gcggagctcg
     3721 aagctccgcg ctacttcccg gcaaagcccc ttcggggctc cccctcccag gcgaccactc
     3781 ccaaaccgcg ggcgatggca accggaaaca gggcatacac caattcctta tctccgaaat
     3841 cacagtccgc cgcgcggcgc ttgacgtgaa cagcctgggt ctgtcatact ggcggggaaa
     3901 ctgcgcatca ccaggagagg aggaagtcat ggatcggggg agagagcatt ggatggtcga
     3961 tgtgcgcgaa gcgatcgcgc ttcagcaaga gttacgcaaa cggatccgcc tggatgatga
     4021 cctggacgag gtgcgcaccg tcgcgggggt cgatacggcc tttccaaaac gcggcggcga
     4081 gccggttgcc cgatgcgccg tggtggtcct ctccttcccg gagctgcgcc cggtggcaca
     4141 ggtgacctcc gttgtcccca tcaccttccc ctacgtgccg gggctgctcg catttcgcga
     4201 ggggcctgcc atcgaggcgg cgctgagtca gctccagcgc ctgcccgacc tcctcctctt
     4261 cgacgggcac ggctacgcgc atccgcggcg catggggatc gccacccacc tcggcgtgct
     4321 cctcgaccgc cccaccctgg gatgcgcaaa aagcatcctg accggccacg ccgaagagcc
     4381 aggccccaac ccgggcgacc gctccccgct gctcgctccc gatggcgagc tgctcgggta
     4441 tgccgtcaga acccggcggg gcgtcaagcc ggtctatgtc tccgcagggc accgcatgcg
     4501 tcacgagacc gccgtcgagc tcgtcttgcg ctgtgcgcgc ggctaccgtc tccccgagcc
     4561 cacgcgcctg gcggacaagc tcgcgggaac agaaatggcg tgagggagta gcagggggga
     4621 atcaaaaaac gcccgcgggg agcggggatg tactccagct gccccgcggg ctacggtgat
     4681 gcatcactcc aggcatccgg caggggcgca ttcatgcgga acggctacga gaggtttccg
     4741 ttccgcgcga gcgcccgttc ggttggcctg aacgctcagg ctccgctgcc gaaagtgccg
     4801 tcgaagttca gggcgctgcc ggagctcttc tgcaccgtcg aggtctcgat cttctccttg
     4861 agccgggcga ggaaaagctc ctcatcgacg gggatctcta cccagtcatc cgtccaggtg
     4921 gagaggagca tggcgttggc gagctggacg ccgttgatgc cctcttcgcc acgggccagg
     4981 aggggagcgc cactggtgat cgcctggatc cagttcttgg tgatcccctt gtgcccctcg
     5041 cctccgccgg ccgggatctc gatctgccac gtctccggct tctggaaacc accctgcgcc
     5101 tcgtagatgt acttctgcac cgaggagcgc gtgcgccaga aggtgagctt gcccccctcc
     5161 agcacgagcc ggccacggtc gccggtgatc tcgaagctgt tggagccggg cgcctcgccg
     5221 gtgctcgtaa taaagacacc cgtcgccccg ttcgggtact cgacgtaggc cgtcacgtca
     5281 tcctcgactt cgatatcgtg gtacttcccg aaagagcaga aggcgcgcac gcgcgagggc
     5341 atgccgcaaa tccactgcca caggtccagg ttgtgcgggc actggttggc aagaacgccg
     5401 ccaccctcac ccgaccaggt ggcgcgccag ccgccggagt tgtagtaggc ctgggtccgg
     5461 aaccaatcgg taatcacgta gtgggtgcgc cggatctcgc ccagttcgcc ggactgcacc
     5521 aactcgcgca tcttctggtg tccggccagc gtgcgctggt tgaacatcag gccaaacact
     5581 ttgccactgc gctctgcggc ctcgttcatc tcgcggactt gcttggtgta gacgcccgcc
     5641 ggcttctcgc tgaggacgtg gcacccgtgc tggaacgcct ggatcgcgag cggcggatgg
     5701 aagtagtgcg gcgtcgcgat caacaccgcg tccaccacgc ccgcggcaaa aagcgcgtcg
     5761 gccgtctcaa aggtcttcac ctggtcgccg aaggccttct tcgccgcttc caggcgctcc
     5821 ggagccacat cacagacggc ggtcagtgtc gcgtcgggca cttctccctt gaaaaggtag
     5881 ttggcatgga agcttcccat gccgccataa ccgatgattc ccacacgaac gctactcatc
     5941 tatccctcct ggtcttgcgc gcattggcac aacgatcgcc atgaagatgt cagaagacgc
     6001 atccttagcc gccctgcaac ccgtggaagc cccctcctgc ggaacggggc ggggattctc
     6061 cgccgcgcgg agggcactcc cgggttcgca agcggaaacc tttacatcga atgctctggc
     6121 gaaccgatcc caggcgctgg tctcatggcc ctgaacagat cacagcccag tcccctattc
     6181 gacacgcgcg tccttttccc caccctggga tcggaagcgc ctctccctct ctcatcccgc
     6241 cttctgggca aagagcgcat cgagctccgc ttcatggcgc agcagcagct ccaccagccg
     6301 gagcgtacgt tcgattgtgg atgcgctttc ccc
//
LOCUS       DAOSCV010000117         6304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7886_length_6304_cov_2.766843, whole genome shotgun sequence.
ACCESSION   DAOSCV010000117 DAOSCV010000000
VERSION     DAOSCV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7886_length_6304_cov_2.766843"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PLY56_04250"
     CDS             <1..348
                     /locus_tag="PLY56_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20722.1"
                     /translation="GVYTLVKNKYFLDELYASVVIAPLFVVSRWAHRFDVKVVDHFVN
                     AVGHVTLLTAILQGKFDKQVVDGAVNGAGWTAGQVGQGFRRLQTGLVQGYALIVFVGL
                     VAATGLYLYFGFR"
     gene            365..1861
                     /locus_tag="PLY56_04255"
     CDS             365..1861
                     /locus_tag="PLY56_04255"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOJ20723.1"
                     /translation="MPQMLLTLMTFVPLVGALAILFVPKERANAIRWTALAFSVVTLL
                     LSVGVAIAFDYGATGIQMATKVQWIQAIPVYYQLGVDGLSLPLILLTTALTSLCIVYS
                     WHIEHRVKEYFFMFLLLETGMLGVFVALDFFLFYVFWEISLVPMYFIIGIWGGERRIY
                     ASIKFFLYTLFGSVAMLLAIVALYLRTGTFDMIEMATMRPYAGDPIWLALVFWGLFLG
                     FAIKVPVFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRIVLPIVPEAARQFAP
                     VVAVLAVISIVYGALVAMAQWDLKKLIAYSSVSHMGYVMLGVAAATYTSSASLASKVT
                     AVNGAALEMVNHGIITGALFFLVGVIYDRTHTRDLKRFGGLGAVTPIYAFFFSVAMFA
                     SLGLPALAGFWSEFLVFVGAFPAIPVHTVASAVGLVLTAAFFLWTIQRVLLGPINPEW
                     KGLTDMDRRELLALTPLLVLIIAIGVYPAPLLNVINGAVTRLLTTMPM"
     gene            1871..3466
                     /locus_tag="PLY56_04260"
     CDS             1871..3466
                     /locus_tag="PLY56_04260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOJ20724.1"
                     /translation="MSSAVIDIRWFWVAPELVLTFAGLAGLLAAAMRTPWARHPFARP
                     AVPPEDAEGTVLRAAEWICIVGVAVAGLLAAAVGWSTRALHPGVMVHFWGGMMHDPFS
                     AFFKVAVAVATLLVMAMTAAYRRSFPNRPEMYALLTLATLAIFFMASASELVVLFLAV
                     EFLSIVSYVLVGYLKNEPRSAEAGIKYFLFGTVCSAVMLYGMSLLYGLTGSTLLQGIA
                     GVLAGETDVARRMVVLAAAFFVLAGLGFKVSMAPFHQWVPDAYEGAPTPITAYLSVAS
                     KAAGFAVTVRFLGVALALPQIAPHWAALIAILAMITMTWGNTAAIWQTNIKRMLAYSS
                     IAQAGYILTGLAAAGVAGEGVREFAIPGILLYVLAYLFMNLGAFAVVIAVANQDGVEE
                     IPGYAGLARRAPWLAAAMTIFLLSLIGIPPTAGFIAKLYLFLAAIKASTPLMTTLAVV
                     MFVNSVISAYYYLNVVRQMYLGEPVAEKRISAHLGLKLTVGVCAVATIAILIAAEPFL
                     ALSQDAVYTSTPLFSILIGRFTW"
     gene            3558..4292
                     /gene="ubiE"
                     /locus_tag="PLY56_04265"
     CDS             3558..4292
                     /gene="ubiE"
                     /locus_tag="PLY56_04265"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOJ20725.1"
                     /translation="MDEKPRRVRAMFARIAPRYDLLNTLLSAGTHKWWRRTALRACRL
                     RPGMTALDVGAGTGDMAIGMARQVGPQGRVIGVDFCEPMLARGQAKLRARGLEGSVLL
                     TGGDAQSLPLRDTSVDAAVMAFVLRNVADVELALCEMARVVRPGGSVVVLELGRPRGA
                     LFRAVYETYFHRILPRVAALLSHKDAYTYLPDSVAAFHSPEEVAAMMARAGLEEVRFR
                     LLMGGVATLHVGVAGGPTGRRPGIPR"
     gene            complement(4431..5999)
                     /gene="dacB"
                     /locus_tag="PLY56_04270"
     CDS             complement(4431..5999)
                     /gene="dacB"
                     /locus_tag="PLY56_04270"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOJ20726.1"
                     /translation="MTTMEINRASQTRESRRCAAGGKKNRLGRAGAGRRRPIIQIALL
                     LLVGLGPLPPHAGAVTEDELGPAIRAAIAVPALEHGVTAVLVQSLRDGRTLYATNPKM
                     ALMPASNAKLFTAILALEHLGPEFRFPTVLAARGKVSQGVLAGDLILKGFGDPTLVTA
                     TLHDFVNAVRAKGIERVEGDLLVDATYFTAPRWGSGWSWDYLEDYYAMEISALSLNNN
                     TMALVIQPGTEAGRPALISWAPPTRHMEVINETTTGAPDSPRTLRYSRELGKNRVVVR
                     GAIPLGSQPAVLDGIPVEDPALYCGHVLSELLRQAGIPVAGRVRYGTLPEREATRIYQ
                     HHSAPLSEILPLFLKPSNNHIGEQLIHTILAVEKARLRQASQSGAQTPPPSIEGLADA
                     LITKSGADPRAVRLVDGSGLSRLNLVTALSVVQLLRYAYQQPYRDVLVRALPVAGNDG
                     TLRRRMRETRAAGNVRAKTGTLNAVSGLSGYVTAASGEPLVFSILMNNFLAGSQPARE
                     AQDRICILLAELER"
BASE COUNT          875 a         2046 c         2155 g         1228 t
ORIGIN      
        1 ggcgtctaca ccctggtgaa gaacaagtat ttcctggatg agctgtacgc gagcgttgtg
       61 atcgcgcccc tcttcgttgt ctcgcggtgg gcccaccgct tcgatgtgaa ggtggtggat
      121 cactttgtca acgccgtggg ccacgtcacg ctgctcaccg cgattctcca gggcaagttc
      181 gacaagcagg tggtcgatgg cgccgtgaac ggggctgggt ggaccgcggg ccaggtggga
      241 cagggtttcc gtcgcctgca aactggcctg gtgcaaggct acgcgctcat cgtcttcgtg
      301 ggcctcgtgg ccgcgacggg tttgtatctg tactttgggt tccggtagag gaaggcacaa
      361 ctagatgccg cagatgctgc tcactctcat gacgttcgtt ccgctcgtcg gagcgctggc
      421 gatcctcttc gtaccgaagg agcgcgccaa cgccatccgc tggacggcgc ttgccttcag
      481 cgtggtgacg ttgcttcttt ccgtcggagt ggcgatcgct tttgactatg gcgccaccgg
      541 cattcagatg gcgacgaagg tgcagtggat ccaggcgatc ccggtctact accagctcgg
      601 ggtggacggg ctgagcctgc cgctgatcct tttgacgacg gcgctcacca gcctctgcat
      661 cgtctattcg tggcacatcg agcatcgggt gaaggaatac ttcttcatgt tccttctcct
      721 ggagacgggc atgctgggcg tcttcgtggc gctcgatttc ttcctcttct acgtcttctg
      781 ggagatcagc ctggtgccga tgtacttcat catcggtatc tggggcggcg agcggcggat
      841 ctacgcctcg atcaagttct tcctctacac gctgttcggc agcgtggcga tgctgctggc
      901 gatcgtggcg ctctacctgc gcaccggcac gttcgacatg atcgagatgg ccacgatgcg
      961 cccctacgcg ggtgacccga tctggctggc gctcgtcttc tgggggctct tcctcgggtt
     1021 tgccatcaag gtgccggtct tcccgttcca cacctggctt ccggacgcgc acgtggaggc
     1081 gcccacagcg ggtagcgtca tcctggcggg cgtcctcctg aagatgggca cctacggctt
     1141 cctgcggatc gttctcccga ttgtgccgga ggccgcgcgc cagtttgcgc cggtcgtggc
     1201 ggtgctcgcg gtgatctcca ttgtctacgg cgccctcgtc gcaatggccc agtgggacct
     1261 gaagaaactg atcgcctact cctcggtcag ccacatgggc tacgtgatgc tcggggttgc
     1321 cgcggccacc tatacgagca gcgccagttt ggcgagcaaa gtgacggcgg tgaacggcgc
     1381 ggccctggag atggtgaacc acggtatcat cacaggcgcg ctcttcttcc tggtgggcgt
     1441 gatctacgac cgcacgcaca cgcgcgatct gaagcgcttt ggcggcctgg gagcggtcac
     1501 cccgatctat gccttcttct tcagcgtggc gatgttcgcc tcgctggggc tgccggcgct
     1561 ggcaggcttc tggagcgagt ttctcgtctt tgtcggtgct ttcccggcga tcccggtgca
     1621 taccgtagcc tcggctgtcg gcctcgtgtt gacggcggcg ttcttcctgt ggacgatcca
     1681 gcgcgtgctt ctcggcccga tcaacccgga gtggaagggc ttgacggata tggaccggcg
     1741 cgagctgctg gcgctcacgc ctctgctcgt cctgatcatt gccatcggcg tgtacccggc
     1801 gccgctcctg aacgtgatca atggcgcggt gacgcggctg ctgaccacaa tgccgatgta
     1861 ggcggatctt atgtcttctg cggtgataga cattcgttgg ttctgggtgg cgccggagtt
     1921 ggtcctcacc ttcgccggcc tggccgggct gctcgcggcc gcgatgcgca cgccttgggc
     1981 gcgccatccc ttcgcgcgcc ctgcggtgcc gccggaggat gccgagggca cggtgctgcg
     2041 cgcggcggag tggatctgca tcgtaggcgt ggccgtggcg ggtcttctcg cggccgccgt
     2101 cgggtggtct acgcgcgcgt tgcatcccgg ggtgatggtc catttctggg gcggcatgat
     2161 gcacgacccc ttcagcgcct tcttcaaggt ggcggtggcg gtggcgacgc ttctggtcat
     2221 ggcgatgacc gccgcctacc ggcgctcctt ccccaaccgc ccggagatgt acgcgctgct
     2281 caccctggcg acgctggcga tcttcttcat ggcgtcggcc tccgagctgg tggtgctctt
     2341 cctggcggtg gagttcctca gcatcgtctc ctatgtgctg gttggctacc tgaagaacga
     2401 gccgcgttcc gccgaggcgg ggatcaagta cttcctcttt ggcaccgtct gctccgccgt
     2461 catgctctac ggtatgtcgc tcctttacgg gctgacgggg agcacgctgc ttcagggcat
     2521 tgccggcgtc ctggcaggag agacggacgt cgcccggcgg atggtggtcc tggcggccgc
     2581 gttctttgtg ctggctgggc tcgggttcaa ggtctccatg gcgcccttcc accagtgggt
     2641 gcccgatgcc tatgagggcg cgccgacgcc gatcaccgcc tacctctcgg tggcgtccaa
     2701 ggcggctggc ttcgcggtta ccgtccgatt cctcggcgtg gcactggcgc tgccgcagat
     2761 cgcgccccac tgggcggccc tcatcgccat cctggcgatg atcaccatga cctggggcaa
     2821 caccgcggcg atctggcaga ccaacatcaa gcgcatgctc gcctactcca gcatcgcgca
     2881 ggcgggctac atcctgaccg gactggccgc cgcgggtgtg gccggcgaag gggttcgtga
     2941 gtttgccatc ccgggcatcc tcctctacgt gctggcctac ctcttcatga acctcggtgc
     3001 cttcgccgtg gtgatcgctg tggccaacca ggatggcgtc gaggagattc cgggctacgc
     3061 cggcctggcg cggcgcgcgc cctggctggc ggccgccatg acgatcttcc tcctgtcgct
     3121 gatcggcatc ccgcccaccg cgggcttcat tgccaagctc tacctcttcc tcgccgcgat
     3181 caaggcgagc acgccactga tgacgacgct cgcggtcgtc atgttcgtca acagcgtcat
     3241 ctcggcgtat tactacctga acgtggtccg gcagatgtac ctgggcgagc cggtcgcgga
     3301 aaagcgcatt tcggcccatc tgggcctgaa gctgaccgtg ggtgtttgcg ccgtggcgac
     3361 aatcgcgatt ctcatcgccg cggagccgtt ccttgcgctg agccaggatg cggtctatac
     3421 ctccacgccg ctctttagca tcctgatagg acggttcacc tggtgaagcc cgcgcgcgag
     3481 tggccggatg cggatagctg ggccattcgc cagggctcgc tcggtaccgg gaaggcccat
     3541 gccagggagg aacggggatg gatgagaagc cgaggcgcgt gcgcgccatg ttcgcgcgga
     3601 tcgcgcctcg ctacgacctc ctgaacaccc tcctcagcgc gggcacgcac aagtggtggc
     3661 gccggacagc cctgcgcgcc tgccggcttc gccccggcat gacggcgctc gacgtaggag
     3721 ccgggacggg cgacatggcg atcggtatgg cccggcaggt gggtccgcaa ggccgcgtga
     3781 tcggcgtcga tttctgcgag ccgatgctcg ctcgcggcca agcgaagctg cgcgcgcgcg
     3841 gcctcgaagg cagcgttctc ctgacagggg gcgacgcgca gtcgcttccg ctgcgcgata
     3901 ccagcgtaga tgccgccgtg atggcgttcg tgctccgcaa cgtggcggac gtggagctcg
     3961 cgctctgcga gatggcgcgc gtggtccgcc cgggcggcag tgtggtcgtg ctcgagctgg
     4021 gccgaccgcg cggcgccctc tttcgcgctg tctacgagac ctacttccat cggattctgc
     4081 cgcgcgtggc cgcgctgctg agtcacaaag acgcctacac ctatctccca gattccgttg
     4141 ccgcgttcca ctcccccgaa gaggtcgccg cgatgatggc ccgcgccggc ctcgaagagg
     4201 tccggtttcg ccttctgatg ggcggcgtgg ccaccctgca cgtgggcgtg gcgggcgggc
     4261 cgaccgggcg gcgccctggg attccgaggt aagaaatccg gtcgagggca ccgagacttc
     4321 tggctcgaat gcccgaatct ctctggcctc tcccgccaca gccccgcggt tcaccgcgag
     4381 cccaacgcgg tgggccatgc ctctcctgca cgctcacttg ctttccgcag ctaccgctcg
     4441 agctcggcga gaaggatgca aatccggtcc tgggcttcgc gcgcgggctg ggagccggcg
     4501 aggaagttgt tcatcaggat ggagaagacg agcggctcgc cgctcgccgc ggtcacatag
     4561 cccgaaagcc cgctgacggc gttgagtgtg cccgtcttgg cgcgcacgtt cccggcggcg
     4621 cgcgtctccc gcatgcgccg tctcagcgtg ccgtcattcc ccgcgaccgg gagcgcccgg
     4681 accagcacgt cgcgatacgg ttgctggtag gcgtagcgga gcagctggac cacgctcagc
     4741 gccgtcacca ggttgaggcg agacagcccg gagccatcga cgaggcgaac agcgcgcggg
     4801 tccgcgccgg acttcgtgat gagcgcgtcc gccaggccct cgatggaagg cggcggggtt
     4861 tgcgcgcctg attggctcgc ctggcgcagg cgtgccttct ccacggcgag gatcgtgtgg
     4921 atgagctgct cgccgatgtg gttgttgctc ggcttgagga agagcggcag aatctccgag
     4981 aggggagcgg agtggtgctg gtagatccgg gtcgcctctc tttccgggag cgtgccgtag
     5041 cggacgcgcc cggccaccgg aatccccgcc tggcggagga gctcggagag aacgtgcccg
     5101 cagtagaggg ctgggtcctc gaccgggatg ccgtccagaa ccgctggctg gctgccgagg
     5161 gggatggcgc cacgcacgac cacgcggttc ttgcccagct cgcgcgagta gcggagcgtc
     5221 cgcggcgagt ctggcgcacc ggtcgtcgtc tcgttgatca cctccatatg acgggtgggc
     5281 ggcgcccatg agatcagcgc tggccggccg gcttccgtgc ccggctggat gacgagcgcc
     5341 atggtgttgt tgttgagcga gagtgcgctg atctccatgg cgtagtagtc ctccaggtag
     5401 tcccacgacc agccgcttcc ccagcgcggg gcggtgaaat aggtggcatc gaccagcaga
     5461 tcgccctcga cgcgctcgat ccctttcgcg cggacggcgt tgacgaagtc gtgcagcgtc
     5521 gccgtcacga gcgtcgggtc gccgaagccc ttgaggatga ggtcgcctgc caggaccccc
     5581 tgcgacacct taccgcgcgc agcgagcacc gtcggaaacc ggaactcggg tccgaggtgc
     5641 tccagggcga ggatggcggt gaacagcttg gcgttggacg ccggcatgag cgccatcttc
     5701 gggttggtgg catagagcgt gcgcccgtcg cgcagcgatt ggacaagcac cgctgtcacg
     5761 ccgtgctcca gggcgggcac ggcaatcgcc gcgcggatgg cggggccaag ctcatcctcc
     5821 gtcaccgcgc cggcgtgtgg gggaagggga ccgaggccga cgagcaagag cagcgcgatc
     5881 tggattatcg gccgccggcg cccggccccg gctcgaccga gacggttctt ctttccgccg
     5941 gccgcgcaac gacgtgactc gcgcgtttgg cttgcccggt tgatctccat tgttgtcatg
     6001 cttctcgccg cgcgcgggaa cgcggcccat ccctcccccc tcctgttcga tggggcggcg
     6061 gtcgctcctc ccgggagggc gcgcggggat ggtccgcggt agcggcgata ccacagatag
     6121 agagggccac ccgcgcgcgg agctggaagc tatgcgctgt ttctcggcaa agccgctttg
     6181 gggctcctcc ttccagctcc cgccatgttg ggttgcccag gagtatggcc cggcccagcc
     6241 tggccgggcc ttgtatatat gcggcggggg ccaggcgttg ctgaagcgct caagggagtg
     6301 gccc
//
LOCUS       DAOSCV010000118         6261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7964_length_6261_cov_3.556880, whole genome shotgun sequence.
ACCESSION   DAOSCV010000118 DAOSCV010000000
VERSION     DAOSCV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_6261_cov_3.556880"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PLY56_04275"
     CDS             complement(<1..1493)
                     /locus_tag="PLY56_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20727.1"
                     /translation="MKKLLILLLLSGLMAPAAQAAQVVAEGPWHVTVGPTGPGEIRYN
                     GQVLVQGVHLTGFQPQWKGSRFHMGGARITAGKAGATWEKRDPGNQEATVAVSAAGPK
                     LTFTLKTAIHAPGPTEWTFVLVPEAVRAAETHCSLRVDDKHSSLNLTDTFPQLSNFRE
                     LRFEQPERTVIIRAEGALLQDRREQGQGLHLVLVIGSDGSQAKSETRAVEIEVLDAKP
                     DEVVARRAVLSQVPIVTRSVPVPNAGFEEEEAFASWTRSPRAALDTEVKRSGKQSARI
                     TIPEEQEDLTGVYLTQQVPVRAGQLYTAEAWVKTQGVAPKELGGRHSTGGTIILEWAD
                     KEGKWLAAGAYAPGSYGNTDWKRVATKECRAPEGAAYAVLFLSMRATGTAWFDDVKLT
                     ETQRHVVLLAPGFQKVVADNTPRFEWSYAERADAILEISSDPGFPEGKTVTIDRVQRA
                     VASPEKPLPPGKYYWRVRIAGHDASSAVWEFQQTAPQDQDCTEPVIEV"
     gene            1792..2556
                     /locus_tag="PLY56_04280"
     CDS             1792..2556
                     /locus_tag="PLY56_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ20728.1"
                     /translation="MITTESIWHQIREGDECARAQVVQNYAHLVKITVSKLVPTLPPN
                     LDQEDLIGAGYMGLIRAVDQFDLGRGVKFGTYAISLIRGAVLDALRKEDWVPRSTREK
                     ARNLQNLYAQLERRLGHPATDEEMAEALGLDIDEFHTLLYETSRTSLVSLDDLLTNSD
                     NDETPLCDSMRGDSPDPQAELERKEMWRALSYSLDTLPERERIVVYLYYFEGLTLKEI
                     GRELQISESRVHQLHGQAVRRLARQLNDRRALFTAE"
     gene            2570..3592
                     /locus_tag="PLY56_04285"
     CDS             2570..3592
                     /locus_tag="PLY56_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ20729.1"
                     /translation="MRCRCRLALVASLAVVSVTVFAGCGKKPAAVVNGKRIERARYEE
                     LLERTAGAPLLVQLISEELILQEAEKQKVMPPPAEVDRRLAEAKKANPSAFQGGAEDE
                     ARRQVLIMLATRNLAFKGLSVPESEVKATFEKHRKQFDQPEAVVVRRAVFKTKKEAET
                     ARQTLMKANVEFGVILGQSIDHQEIRQNGGALPALIRTTPGGPITMILGSDPQTGAAI
                     QRPADLLLGQGVAEKAFNLPEKTIMEVLPSQSPKGYQVIQVQEHREAKRGTYAEWQER
                     IREQLLLEKRFKDEKIPEGVDPITYYQAKLVSDLRRKANIEINIERFKELPKHPEAWP
                     NVGGGQ"
     gene            3598..5043
                     /gene="mazG"
                     /locus_tag="PLY56_04290"
     CDS             3598..5043
                     /gene="mazG"
                     /locus_tag="PLY56_04290"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ20730.1"
                     /translation="MVGLGPGSLGGLSVSALETLTRAPRVLLRTRQHPVVDALEAKGM
                     RAESYDSLYESACSFDELYQRIAGAVLEEARAGDVVYAVPGSPLLGERTVTLLVEQCE
                     AEGIPLTLIPSVSSLDATLAVLRVAVNTALEVLHAPDVARFRPEGIAPLFIFGLYDVA
                     VASEVKLSLMEIYPDDYPVTVVRGAGVDGRNEQETIPLYCLDRVSVDHLTSLFVPAME
                     RRPATSFERLVEVMAALRAPNGCPWDREQTHESLKKYLTEETYEFFDAIDDQDDERMA
                     DELGDLLLQMVFHARIAEEEDRFDIHDAIRSIVEKLIRRHPHVFGDTEVSGADEVLDN
                     WEKIKAGERLTAHRKSLLDAVPRAMPSLQRAFELTRRAAKVGFDWKEATDVLDKVGEE
                     MEEAREALAAGDQAAVRHEVGDLLFALANLARKAGVDPEDALHAANRRFSDRFRYIEA
                     RAQEQGTSLAEMSLEEMDRLWNEAKARLDGA"
     gene            5425..>6261
                     /locus_tag="PLY56_04295"
     CDS             5425..>6261
                     /locus_tag="PLY56_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ20731.1"
                     /translation="MLRAIMGAVSTGIAVVDALTGEILASNERMAELLGGPISRHIRM
                     AEWIEAKGFHPDGRRYLPEEWPSARAMRGEVVTAEEIAVERSDGTCVALSISAVPFRN
                     PRGRVLAAVLVVEDISERKRIEGALRQANMELQAANRELDAVATELRASEEQMRALYE
                     QTPAIVVLSHLDNGRYVRVNEAFEHVLGWSREEVVGRTSVEMGIFARPEEREHYANLV
                     REHGRVRDLEATLHARDGRALTALLSGSAVEIGGEPYLITVAVEITERKQAEAALRRY
                     ELI"
BASE COUNT         1098 a         1976 c         2084 g         1103 t
ORIGIN      
        1 acctcgatga ccggctcggt gcagtcctga tcctgcggcg ccgtttgctg gaactcccac
       61 acggcggagc tggcgtcgtg gccggcgatg cgcacccgcc agtagtactt gccgggcggc
      121 aggggcttct ccggcgaagc cacggcacgc tgcacgcggt cgatggtgac ggtcttgccc
      181 tcggggaaac cgggatcact ggagatctcc aggatggcat cggcgcgctc ggcgtaggac
      241 cactcaaagc ggggggtgtt gtccgccacc accttctgga agccgggcgc cagcaggacg
      301 acgtggcgct gcgtttcggt gagcttcacg tcatcaaacc aggccgtgcc tgtggcgcgc
      361 atggagagga agaggactgc gtaggcggcg ccttccgggg cgcgacactc cttcgttgct
      421 acccgcttcc aatcggtatt gccgtaggag ccgggggcat aggcgccggc ggcgagccac
      481 ttgccctctt tatcggccca ctccaggatg attgtgccgc ccgtagagtg ccggcctccc
      541 agctccttgg gcgccacgcc ctgggttttc acccacgcct cagcggtgta gagctgccca
      601 gcgcgaaccg gcacctgctg cgtcaggtag accccggtga gatcttcttg ctcctcaggg
      661 atggtgatcc gggcgctctg cttcccgctc cgcttcacct ccgtgtcgag cgcagcccga
      721 gggctgcgcg tccacgacgc gaacgcctcc tcctcttcga agccggcatt tggcacgggc
      781 acggagcgag tgacgatggg aacctgcgag aggacggccc ggcgtgccac cacctcgtcg
      841 ggcttcgcat ccaggacctc aatctcgacg gcgcgcgtct ccgacttcgc ctggctccca
      901 tcggagccaa tcaccaggac gaggtgaagg ccctggccct gttcgcgccg gtcctggagc
      961 aaggcgccct cggcgcggat gatgacggtg cgctccggct gctcgaatcg cagctcgcga
     1021 aagttcgaca gctgggggaa ggtgtcggtc aggttgaggc tgctgtgctt gtcatctacc
     1081 cgtagcgagc aatgggtttc cgcggcgcgc accgcctcgg ggaccagcac gaacgtccac
     1141 tccgtcgggc caggggcgtg gatcgctgtc ttcagcgtga acgtgagctt cgggccagct
     1201 gcggagaccg ccacggtagc ctcctggttg ccggggtcgc gcttctccca ggttgcgccc
     1261 gccttgccgg cggtgatgcg cgccccgccc atatggaacc ggctgccctt ccattggggt
     1321 tggaacccgg tcaggtgtac cccctgaacc agcacctggc cgttgtagcg aatctcgccc
     1381 ggacccgtcg ggcctacggt gacgtgccac ggcccctcgg cgacgacctg cgccgcctgc
     1441 gctgcaggtg ccatcaaacc cgatagcagg agcaaaatca ggagcttctt catcgccatc
     1501 cacctccaca gggtgtcggt ttccgatccg caaatctttg cccggatcct tcttcgcctc
     1561 ggtgcttacc tccttgccgc ttgtggacgc gagggcacat cgatcgcccg gggcgtgtcg
     1621 cccgtagaca aatcaccgcg ctgcttgtga tcgaagccct gtcaggctgg gttaggtcgc
     1681 acgcgcgggc agcgcacgcc tgttgacctg cgcctgttgg cacggatcgt tcacttgaca
     1741 agggatccct tttcgagtag aatcggggca acgaccaccg ggatcaaatc tgtgatcact
     1801 acagagtcta tctggcacca gattcgagag ggcgatgagt gtgcgcgtgc gcaggtagtt
     1861 caaaactatg cgcatcttgt gaagatcaca gtctcgaagc tggtgccaac gctgccaccc
     1921 aatctggatc aggaagacct gatcggcgcg gggtacatgg gcctcatccg ggctgtagat
     1981 cagtttgatc tggggcgcgg agtgaagttc gggacgtatg ccatctcgct gatccgggga
     2041 gccgttctcg atgccttgcg caaagaggat tgggtgccgc gctcgacgcg cgagaaagcg
     2101 cgcaacctcc agaatctcta cgcccagctc gagcgccgcc tcggacatcc cgccacagat
     2161 gaggagatgg cagaggcgct tgggctcgac attgatgaat tccatacgct tctctacgaa
     2221 acgagccgca ccagcctggt atcgctggat gacttgctga cgaattccga taatgacgag
     2281 acgccgctct gcgacagcat gcgtggcgac tcaccggacc ctcaagcgga gctcgagcgc
     2341 aaagagatgt ggcgcgctct ttcctactcg ctcgatacac tgccggaacg cgagcgcatt
     2401 gtggtgtatc tatactactt tgagggactc accctcaaag agattggccg tgagctccag
     2461 atctcggaat cgcgcgtaca ccaactacac ggacaggcgg tccggcgcct ggcccgccaa
     2521 ctcaacgatc ggcgagcgct tttcacggcg gaatagggag atattggcta tgcgttgtcg
     2581 ttgccggctg gccctggtgg ccagccttgc ggtggtgtcg gtgacggtgt ttgcgggctg
     2641 cggcaagaag cccgccgccg tagtcaatgg taaacgtatt gagcgcgcgc gctatgaaga
     2701 gctgttggag cgcacagcgg gtgccccgct tctggtgcaa ctcatctccg aggagctcat
     2761 cctgcaggaa gccgaaaagc agaaggtgat gccgcccccc gcagaggtgg accgccggct
     2821 cgccgaagcg aagaaagcca atccgagcgc tttccaggga ggcgccgagg atgaggcgcg
     2881 ccgccaggtg ctgatcatgc ttgccacgcg caaccttgcc ttcaagggcc tgagcgtgcc
     2941 ggagagcgaa gtcaaggcga ccttcgagaa gcaccggaag cagttcgacc agcccgaggc
     3001 cgttgtggtc cgacgcgccg tcttcaagac caagaaggag gccgaaaccg cgcggcagac
     3061 tctgatgaag gcgaacgtcg agtttggcgt cattctcggt cagtcgattg accaccagga
     3121 gatccgccag aacggcggtg ccctcccggc tctcatccgc acgactcccg gtggcccgat
     3181 cacgatgatc cttgggtcgg acccgcagac cggcgccgca atccagcgcc cagccgacct
     3241 cctccttggc cagggagttg ccgaaaaggc gttcaacctg cccgagaaga ccatcatgga
     3301 ggtgcttccc tcccaaagcc ccaagggcta ccaggtgatc caggttcagg aacaccgcga
     3361 ggcgaagcgc ggcacgtatg ccgagtggca agagcgcatc cgcgagcaac tgctcctgga
     3421 gaagcgcttc aaggatgaga agatcccgga aggggtcgat ccgatcacct actaccaggc
     3481 caagctggtg agcgatctgc gccgcaaggc gaatatcgag atcaacatcg agcgcttcaa
     3541 ggagctgccc aagcaccccg aagcgtggcc taatgtggga ggtggccaat agttactatt
     3601 gttgggctgg gaccggggag cctcggcggg ctctcggtct cggctctcga gacgctcacc
     3661 cgtgctccgc gcgtgctgtt gcgcacgcgc cagcacccgg tggtggacgc tctcgaagcg
     3721 aaggggatgc gcgccgagtc ctacgattcc ctctacgaga gcgcgtgctc tttcgatgag
     3781 ctgtaccagc gcatcgccgg cgccgtcctt gaggaggcgc gcgccggcga tgtggtctac
     3841 gctgtgcctg gatcccctct cctcggcgag cgcacggtga ccctcctggt cgagcagtgc
     3901 gaggccgagg gaatccccct cacgctgatc ccctcggtga gctcgctcga tgccacgctc
     3961 gccgtgctgc gcgtggctgt taacaccgcg ctcgaggtcc tccatgcccc ggatgtggcc
     4021 cgcttccgcc ccgagggcat tgcccccctc ttcatcttcg ggctctacga cgtcgccgtt
     4081 gccagcgagg tcaagctgtc gctgatggag atctatccgg atgattaccc ggtgaccgtg
     4141 gtgcgtggcg ccggggtgga tgggcgcaac gagcaggaga cgatcccact ctactgcctc
     4201 gaccgggtga gcgttgacca tctgaccagc ctctttgtgc ccgccatgga gcgccggccg
     4261 gccacatcct tcgagcgcct ggtcgaggtg atggctgccc tccgtgcgcc gaacgggtgc
     4321 ccgtgggacc gtgagcagac ccacgagtca ctgaaaaagt acctcacaga agagacctac
     4381 gagttcttcg atgccatcga cgatcaagat gacgagcgca tggcggatga actgggcgat
     4441 ctcctcttgc agatggtctt ccatgcgcgc atcgccgagg aggaggaccg ctttgatatc
     4501 cacgatgcca tccgcagcat cgtggagaag ctgatccgcc ggcaccccca cgtctttggt
     4561 gacaccgagg tttctggcgc cgatgaggtg ctggacaact gggagaagat caaggccggc
     4621 gagcgcctga ccgcgcatcg gaaatcgctg ctggacgctg tcccccgcgc gatgcccagc
     4681 ctccagcgtg cgttcgagct gacgcgccgt gccgccaagg ttggcttcga ttggaaagaa
     4741 gccaccgatg tcctcgacaa ggtgggcgag gagatggagg aagcccgcga ggcgcttgca
     4801 gccggggatc aggcggcggt acgccacgag gttggcgacc tcctctttgc cctggcgaac
     4861 ctggcgcgca aggcaggcgt cgatccggag gatgccctcc acgccgcaaa ccgtcgcttt
     4921 agcgaccggt ttcgctatat cgaggcccgg gcgcaagagc agggcacttc gctggctgag
     4981 atgtcgctgg aggagatgga ccgtctctgg aacgaggcca aggcgcgcct ggacggcgca
     5041 tagccccccg gatcactggc agcctgcccg cgcttcgctc cccggccttg ggattccagg
     5101 gccttcgcct tctctcgggt tctgctaccc cccttttgtg caacggactc tgctgttcgg
     5161 gcgatggcac cagagccccc tgcgttctgg gtataatggg aaatgtcggg cgctcgcaac
     5221 catccgggga gtgagatcac cccgcggtga aggtgagaga gcagagtggg agacgcaaga
     5281 agaggatcaa agcgaggcag gcgccgtgtg tatccagagg ggggatcgcg cggcgttcgt
     5341 gccaatcgcc gcgggctccg gcgacagact ccatcctggg tagagcaggc ctcgcgcccg
     5401 caccacgttc tcgcggcaga agagttgctc cgggcgatca tgggcgcggt ttcgacgggg
     5461 atcgcggtcg tggatgcgct caccggggag atcctggcga gcaatgagcg gatggccgag
     5521 ctcctcgggg ggccgatctc tcgccatatc cggatggcgg agtggatcga ggcaaaggga
     5581 ttccaccccg acgggcgccg ctatctgccg gaggagtggc caagcgcaag ggcgatgcgc
     5641 ggcgaggtgg tgaccgcgga ggagattgcg gtcgagcgct ctgatggcac ctgcgtggcg
     5701 ctctccatca gcgccgtacc tttccgaaac ccgcgggggc gcgtgttggc cgccgttctc
     5761 gtcgtcgaag atatcagcga gcgcaagcgg atcgagggcg cgctgcgcca ggccaacatg
     5821 gagctgcagg ctgccaatcg ggagctggat gccgtcgcga cggagctgcg cgcgtcggag
     5881 gagcagatgc gcgcgcttta cgagcagact cccgccatcg tggtgttgag ccatctggac
     5941 aacggccgct atgtccgcgt gaatgaggcg tttgagcacg tgctagggtg gtcgcgcgag
     6001 gaggtggtgg ggcgcacctc ggtggagatg gggatcttcg cgcgtccgga ggagcgcgag
     6061 cactacgcga acctggtccg cgagcatggt cgagtccgcg acctggaagc gacgctacac
     6121 gcgcgcgatg ggagggcgct caccgcgctc ctctccgggt ccgcggtgga gatcgggggc
     6181 gagccctatc tcatcaccgt ggcggtggag attaccgagc gcaagcaggc ggaggccgcg
     6241 ctgcgccgct acgaattgat c
//
LOCUS       DAOSCV010000119         6253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7972_length_6253_cov_3.567602, whole genome shotgun sequence.
ACCESSION   DAOSCV010000119 DAOSCV010000000
VERSION     DAOSCV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7972_length_6253_cov_3.567602"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PLY56_04300"
     CDS             <1..1984
                     /locus_tag="PLY56_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20732.1"
                     /translation="NFMFLRKPAYSFFWDWGPELPVSGIFRPVYLRAFDHAEIADFHV
                     RYKLNGRRVAGTVTVEAPGGEGARAQVSIGGACSSAPVVDGRARVPFALDGVNLWYPN
                     GEGEPFLYDLQITLEGDGILDERRHRIGFRTIEVVRDKRRDGRGTRFAFKVNGKEIFC
                     RGYNWIPVDNSVPRGNPHLYEGNLDLAREGNVNMLRIWGGGYYEDDAFYRMCDERGIL
                     VWQDGAFACSMYPDTDPSFIHSVEAELIYNIKRLRNFTSLALWCGENECHWGYDSWWV
                     GLRQEFPRFFGTEIYDRRFPALVAELDPDRFYWNGSPYSEDPNVSANDPFQGDSHFWD
                     LHGQCQDLSGYLHTAPSFVSETGIQSLPDLRTALTIGEEEDRHLQSFVFDTRNHFESP
                     AKNDRLLKFTGALFRVTGEFEHAVVLSNLAHAEYLKYAVEHWRSQAPDCAGVLIWQLN
                     DCWPAISWSAIDYNLIPKACHYYLRRAFQPDIVGYIQRYSIDYNPDVNSSGNLFVASE
                     RDGVKVGEVELRVVRVDGKVLETLCFPVRLEGRGAVSLGEVALPEYRARRFDCVAEFT
                     LRWEDGVTARNVYTYSRPKHMRLACPTIEVTQVGPDRLALRTDRFAKGVYLFHPEREV
                     VFSDNYFDLMPGEERVVHMTRPAAASDIRVYAYHHG"
     gene            2156..3463
                     /locus_tag="PLY56_04305"
     CDS             2156..3463
                     /locus_tag="PLY56_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ20733.1"
                     /translation="MIPDVSGARALAHVREICERFGSRPAGSEKERKAAEHIAARFRE
                     FGLSEVVIEPFPCLTWDYSRASLTVAGAEIPCLPVAHSPATPAGGIEAELVYLERASD
                     IDLARVPLAGKIGLLSTLYGDDSERLRRLMDSGLAAVILVDDRLPFDWTVAVGVPAQW
                     IRLLSIPMVSIPYRHAWEIVRNGAQRARLEVATRVREAESQNVVGVVPGSDPTAGDIL
                     LTCHHDTVMNAVGAEDNGSGVACVLELARAFAGSRPRRTLRFVACGCEEQLSEGSKEY
                     VLRHRDEMPGAQFVLNTDSVGGWMGQNEVTVSGCPEMYRWLEGAQQATGFSAKLLDQV
                     SPYSDHFPFTAVGVPGIWVHRTNCAGGRFFHHSALDTPDVLSPGVLDATLRFQARLLN
                     DLAHAEELPFPRAVPDAQREVIARFWRDLYGVADQGDGAAPIR"
     gene            3589..4890
                     /locus_tag="PLY56_04310"
     CDS             3589..4890
                     /locus_tag="PLY56_04310"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HOJ20734.1"
                     /translation="MPRKPEGSISVSGRLSIRQALLEWRHPLLRYLNRRDGAYAWSRA
                     GEVEIPSMGNAVRLHLLSKVWQGVPWKHELALYPPAGPQDASIALLLVTGNRGDLPTL
                     ALMARAARLLQIPAAVLFDVPNQPLFGGKSEDALIAHTFLECLRSGDRSWPLLYPMVK
                     GVVRAMDAIQEFAAREWRTEIAGFVVTGASKRGWTTWLTGACDRRVVGIAPLVYDNLN
                     VTAQMAHQMAVYGSYSEEVADYSAVGLPQKLVTPEGRKLANMVDPFHLRDRLEIPKLM
                     IHGSNDRYWTLESANLYFDELPGDKHILYVPNSGHRLAEMPKVLSALGAFVDACAAGR
                     TLARLEWSCHEVPEGLRLTLTSEHHPERVTIWTAAAETRDFRDAFWRPRRITGRGERY
                     EYLLPRPNRGYAALFGEVVLRWTRGTYAQSTTPHVVAAIAS"
     gene            5135..5923
                     /locus_tag="PLY56_04315"
     CDS             5135..5923
                     /locus_tag="PLY56_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ20735.1"
                     /translation="MGPTLPQLFSLEGKVALVTGGAGFLGSAMCRGLAQAGARVVVAS
                     RTLEKCRRLAEELGPQHRAVAIDLSDETNIRTTIDQVAAEMGRLDILVNNGYNGPLAR
                     LDTATGDDFARSLAVGVTGQFIAAQQAARQMRRVGGGSIINVASMYGLVASYPEVYEG
                     LETCSPPNYHATKGALIQLTRHLAAYWARDGIRVNCISPGAFPHPPVRERMPEFIRRL
                     SERVPMKRMGEPHELMGAVVFLASDASSYCTGHNLVVDGGWTIW"
     gene            5920..>6253
                     /locus_tag="PLY56_04320"
     CDS             5920..>6253
                     /locus_tag="PLY56_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20736.1"
                     /translation="MSEKIPVLVVGAGSIGERHARCFLATERTSVAICESRADRLDDL
                     QAAYPLSAAYTAWDAVPLRDYAVVVICTPAPFHEAMATRALNEGCHVLVEKPLTLDWL
                     AAERLREAA"
BASE COUNT         1021 a         2011 c         2118 g         1103 t
ORIGIN      
        1 caacttcatg ttcctgcgca agccggcgta ctcgtttttc tgggactggg gtccggagct
       61 accggtgagc ggcatcttcc gtccggtcta cttgagagcg ttcgatcacg cggagattgc
      121 cgactttcac gtgcgctaca agttgaacgg ccgccgcgtc gccggcacag tgacggtaga
      181 ggcgccgggc ggtgagggcg cccgcgctca ggtgagtatc ggcggtgcgt gctctagcgc
      241 ccccgtggtg gatggacggg cacgagttcc cttcgcgctc gatggggtca acctctggta
      301 cccgaatggg gagggggagc cgttcctcta cgacctgcag atcacgttgg agggcgatgg
      361 catcctggac gagcgccgcc accggatcgg cttccgaacc atcgaggtgg tgcgtgataa
      421 gcgccgggat ggtcggggca cgcgctttgc cttcaaggtg aatggcaaag agatcttctg
      481 tcggggttac aactggatcc cggtggacaa ctcggtgccg cgcggcaatc cccacctcta
      541 tgaagggaac ctcgacctgg cgcgcgaggg caacgtcaac atgctgcgca tctggggggg
      601 cggctactac gaggacgatg ccttctaccg gatgtgcgac gagcgcggca tcctggtatg
      661 gcaggatggg gctttcgcct gctcgatgta ccccgatacg gatccgagct tcatccactc
      721 cgtcgaagcc gagctgatct acaacatcaa gcgtctgcgc aacttcacgt ctctggcgct
      781 ctggtgcggc gagaacgagt gccattgggg ctacgacagc tggtgggttg gcctgcgcca
      841 ggagttcccg cggttcttcg gaaccgagat ctacgaccgg cgcttcccgg cgctggtggc
      901 cgaactggac cccgaccgct tctattggaa cggctcgccc tattccgagg atccaaacgt
      961 ctcggcaaat gaccccttcc agggcgattc ccacttctgg gacctgcatg gccagtgcca
     1021 ggatctctcc ggctacctgc acaccgcgcc cagctttgtc agtgagaccg ggatccaatc
     1081 gctccccgac ctgcgcacgg cgctgacgat tggggaagag gaggaccggc accttcagtc
     1141 tttcgtcttc gatacgcgca accacttcga aagcccggcc aagaacgacc gcctcctgaa
     1201 gttcaccggg gcgctcttcc gggtgacagg cgagtttgag cacgcggtgg tcctctcgaa
     1261 cctggctcat gcggagtatc tgaagtacgc cgtggagcat tggcgctcgc aggcgcccga
     1321 ctgcgccggc gtgctcatct ggcagctcaa cgactgttgg cccgccatct cgtggtccgc
     1381 tatcgactac aacctgattc cgaaggcgtg ccactactac ctgcgccggg cgttccagcc
     1441 ggatatcgtc ggctacatcc agcgttattc tatcgactac aatccggatg tgaacagcag
     1501 cggtaacctt ttcgtggcct ccgagcgtga tggcgtgaag gtgggcgagg tggagctacg
     1561 cgttgtccgg gtggatggca aggtgctgga gactctgtgc ttcccggttc gcctggaggg
     1621 gcgcggcgcc gtctccctgg gcgaggtggc gcttccggag tatcgagcgc ggcgcttcga
     1681 ctgcgtggcc gagttcaccc tccggtggga agatggcgtc accgcgcgca acgtctacac
     1741 ctactcgcgt cccaagcaca tgcgcctggc gtgcccgacc atcgaggtga cgcaggtcgg
     1801 ccccgaccgg ctggcactcc gcaccgatcg cttcgccaaa ggggtctacc tcttccaccc
     1861 ggagagagag gtcgtcttct ccgacaacta cttcgacttg atgccgggcg aggagcgggt
     1921 cgtccatatg acccgcccgg ccgcggcgag cgacatccgg gtatacgcct atcaccatgg
     1981 gtgatctcgc cccattggcg attcgccgat gagccaggcc gtccccgata gggtgatacc
     2041 acgaatgaag cggacgggcg cgtgtgggga aggcacgagt ggcgcggggc tggaacagga
     2101 gggaggggtg cctgcgtaga agagacactc cactcaccca tgcgaaggag gagccatgat
     2161 tccggatgtg tccggcgcgc gtgctctggc acacgtgcgt gagatctgcg agcgttttgg
     2221 ctcgcgaccg gcgggaagcg agaaagagcg aaaagcggcg gagcatatcg ccgcccggtt
     2281 ccgtgagttc ggcctctccg aggtcgtgat cgagccgttt ccctgtctca catgggacta
     2341 ctcgcgtgcg tcactcaccg tggcgggagc cgaaatcccg tgcctacccg tggcgcactc
     2401 cccggccacc ccggcgggag gaatcgaggc ggagctggtc tatctggaaa gagcatccga
     2461 tatcgacctg gcgcgcgtgc cgctcgcggg gaagatcggc ctgctctcga cactctacgg
     2521 cgacgactcc gagcgcctgc gtcggctgat ggactcgggc ctggcggccg tcatcctggt
     2581 ggacgatcgt cttcccttcg attggacagt cgccgtaggc gttccggcgc agtggatacg
     2641 cctcctatca atcccgatgg tctccatccc ctaccgtcac gcctgggaga ttgtgcgcaa
     2701 cggcgcgcag cgcgcgcgcc tggaggtcgc cacccgggtg cgcgaagcgg agtcgcagaa
     2761 cgtggtgggc gttgtgcccg gcagcgatcc cacggcaggc gatatcctcc tcacgtgcca
     2821 ccacgatact gtcatgaacg ccgtgggcgc cgaagataac ggcagcggcg tggcctgcgt
     2881 gctggagctg gcgcgcgcgt tcgcgggaag ccggccccgg cgcaccctcc gcttcgtcgc
     2941 gtgcggttgc gaagagcagc tctccgaggg ctcgaaggag tatgtcttgc gccatcggga
     3001 cgagatgccc ggcgcccagt tcgtcttgaa caccgatagc gtcggcgggt ggatggggca
     3061 gaacgaggtg accgtgtcgg gctgcccgga gatgtatcgc tggctggagg gcgcgcagca
     3121 ggcgaccggc ttcagcgcga agctcctgga tcaggtctcc ccctacagcg accacttccc
     3181 cttcaccgcc gtgggggttc cggggatctg ggttcaccgg accaactgcg cgggcggacg
     3241 cttcttccac cacagcgccc tcgatacgcc cgacgtgctc tcgccgggcg tcctcgacgc
     3301 cacgcttcgc ttccaggccc gattgctgaa tgatcttgca cacgccgagg agctgccctt
     3361 cccgcgtgcc gtgccggatg ctcagcgcga ggtgatcgcc cgcttctggc gcgacctgta
     3421 cggcgtcgcc gaccagggcg atggagccgc tcccatcagg tgaatccccg aaccatgcgg
     3481 gcccccggcg acgggggccc gttgaaagtg gggaacgcgc cccgaaaccg gatgtagaca
     3541 gcggtagaga acgccgcaaa cgggtatagg gcagaggagg tcaatctgat gccacgcaaa
     3601 cccgaaggca gcatctcggt ttcagggcgc ctctctatcc ggcaagccct gctggagtgg
     3661 agacacccgc tgctgcgcta tctcaatcgc agggatggcg cctacgcctg gagccgggcg
     3721 ggggaggtgg agatcccttc gatggggaac gcggtgcgcc tccacctcct ctccaaggta
     3781 tggcagggag tgccctggaa gcatgaacta gccctctacc cgcccgccgg tccgcaggat
     3841 gcctccatcg cgctgttgct ggtaaccggc aatcgcgggg acctgccgac tctggctctg
     3901 atggcgcgcg cggcccggct gctccagatc cctgcagctg tcctcttcga tgtgcccaac
     3961 cagccgctct tcggggggaa atccgaggac gccctgatcg cgcacacctt tctggagtgc
     4021 ctccgctccg gcgaccggag ctggccgctc ctctacccga tggtcaaggg cgtcgtgcgc
     4081 gccatggatg cgatccagga gtttgccgcg cgcgagtggc gaacggagat cgccggtttc
     4141 gtcgtcacgg gggcttccaa gcgcgggtgg actacgtggc ttacaggcgc ctgcgaccgc
     4201 cgggtcgttg gcatcgcgcc cctggtgtac gataacctga acgtcaccgc gcagatggcg
     4261 catcagatgg ccgtctacgg atcctatagt gaggaggtcg cggattactc cgcggtggga
     4321 ctgccgcaga aactggtgac ccccgagggt cgaaagctcg ccaacatggt cgatcccttc
     4381 cacctgcgcg accggcttga gatcccgaag ctaatgatcc acggcagcaa cgaccgttac
     4441 tggacgctcg aatcggcgaa cctctacttt gacgagctgc cgggagataa gcacatcctc
     4501 tacgtgccga actctggcca ccgcctggcg gagatgccca aggtgctttc ggcgctcggc
     4561 gcctttgtcg acgcctgtgc cgccgggcgc acgctcgccc ggctggagtg gtcctgccac
     4621 gaggtgccgg aaggcctgcg cctgacgctc acttcggaac accatccaga gcgcgtcacc
     4681 atctggacgg cagccgctga gacgcgcgac ttccgggacg cgttctggcg gccgcgtcgc
     4741 atcactggcc gaggcgagcg gtatgaatac ctactgccgc gtccgaaccg cggctatgcg
     4801 gctctcttcg gcgaagtggt ccttcgatgg acgcggggca cctacgcgca atcgaccacg
     4861 ccccatgtgg tagctgcaat cgcctcgtga agagcggcag gagaatgcaa gcgggcgcgg
     4921 aactgggctg caaaggaatc gcgcctttag ccctggcgcc tggtcttgcc atggggcacg
     4981 cccgcgcttg cagcggttga ggcccggccg gctggcttcc gcgagttcgc tcctttgagg
     5041 acgccggcct caccgcgagg tcgagaccct gccgcgaagc ggcgccgcgc tcttctgcgc
     5101 gggcggctcg ccgcccctgg aacggaggag tgagatgggt ccaacactgc cgcagctctt
     5161 ctcgctggag gggaaggtcg cgctggtgac cggcggggcc gggttcctgg gatccgcgat
     5221 gtgccggggg ctggcgcaag cgggcgcgcg tgtcgtggtc gcctcgcgca cgctggagaa
     5281 atgccgccgg ctggccgagg agctggggcc acagcaccgg gccgtggcaa ttgacctgag
     5341 cgacgagacc aatatccgta ccaccatcga tcaggtcgcc gccgagatgg gccggctgga
     5401 tattctggtc aacaatggct acaacgggcc gctggcccgg ctcgataccg ccacggggga
     5461 tgactttgcc cggtcgctgg cagtgggcgt caccggacag tttatcgccg cgcagcaggc
     5521 ggccaggcag atgcgccgcg taggtggggg gagcatcatc aacgtcgcca gcatgtatgg
     5581 cctggtggcg agctatcccg aggtgtatga gggcctcgag acctgcagcc cgcccaacta
     5641 ccacgcaacg aagggagcgc tgatccagct cacccgccat ctggcggcat attgggcgcg
     5701 cgatgggatt cgcgttaact gcatctcccc gggggcgttc ccgcatccgc cggtgcgcga
     5761 gcgtatgccc gagtttattc ggcgtctcag cgagcgcgtg ccaatgaagc ggatgggaga
     5821 gccgcacgag ctgatgggcg cggtggtctt cctggcttcc gatgcatcga gctactgcac
     5881 ggggcacaac ctcgtggtgg atggggggtg gaccatctgg tgagcgagaa gatcccggtt
     5941 ctggtggtgg gcgccggctc gattggcgag cgccacgccc gctgcttcct ggcgacggag
     6001 cgaacgagtg tcgccatctg cgagtcgcgt gccgaccggc tggacgatct ccaggcggcc
     6061 tatccgctct cggcggccta cacggcgtgg gatgcggtcc ccttgcgcga ctatgcggtg
     6121 gtggtgatct gcaccccggc gcctttccac gaggcgatgg ccacgcgcgc gctgaacgag
     6181 ggctgccacg tcctcgtcga gaagcctctg acgctcgact ggctcgcggc ggagcgcctg
     6241 cgcgaggcgg ccg
//
LOCUS       DAOSCV010000120         6234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_7999_length_6234_cov_3.244376, whole genome shotgun sequence.
ACCESSION   DAOSCV010000120 DAOSCV010000000
VERSION     DAOSCV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_6234_cov_3.244376"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..1386
                     /locus_tag="PLY56_04325"
     CDS             379..1386
                     /locus_tag="PLY56_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ20737.1"
                     /translation="MEYRTLGRHGVKVSRLCLGTMMFGGPTPEEEAIRIIHRALDLGI
                     NFIDTADMYTGGESERITGKAIRDRRDQVVLATKVRYPVGGGPNDSGLSRYHIFNAVD
                     ASLRRLGTDHIDLYYLHLPDYETPLEESLSAMDDLVRQGKVRYIGCSNFYAWQICKGL
                     WISDRRGWAPFACVQPLYNIVNRDAEVEVLPLCAEEGLGVVTYSPLARGVLSGKYLPG
                     GDLPPDSRAARGDKRLHETELRQESFEVATRLKALASVKGCTLTQLALAWVMANPIVT
                     CPILGPRTMEQFEDNIGCLDVTITPEDEATIDALVPRGEHTGYGFNDPQYPVRGRPVA
                     A"
     gene            complement(1407..1841)
                     /locus_tag="PLY56_04330"
     CDS             complement(1407..1841)
                     /locus_tag="PLY56_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20738.1"
                     /translation="MGTWRASPEPGAPGASRTVDVLLADDEPLFTGTLCDLLVDQGYA
                     VTTASDGMSAVHLTRCYRPAIVFLDLAVPGIGGEDACRLILAEPDPPRVVILTAHATE
                     EQVRRLLTSGATAVLVKPIPSEQILRLLGEACQARAVAAYSA"
     gene            2209..3063
                     /locus_tag="PLY56_04335"
     CDS             2209..3063
                     /locus_tag="PLY56_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HOJ20739.1"
                     /translation="MREVACVNGQFMPLAEACISVNDRGFLFGDSVYEVARTYEGRVW
                     ALDRHLERLRRSLEGIQIEGVDLNAVRANMLEAYQRSGFDNAVIYVQITRGAGPRSHV
                     PDGPMKPTILITVRLCKGAREEQVRNGVEVILVDETRWARRDIKTTNLLPNILALQQA
                     HARGAHEAVFVTDDGWVHEASHSNLFIVSGGVVRTPPKGPALLPGITRDLVLECARRA
                     GIPAEELPVSREALLSADEVFLTGTSSEVLGVVQIDGQPVASGTVGPITRRLHALYQD
                     RVAAGDDA"
     gene            complement(3148..4449)
                     /gene="melA"
                     /locus_tag="PLY56_04340"
     CDS             complement(3148..4449)
                     /gene="melA"
                     /locus_tag="PLY56_04340"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ20740.1"
                     /translation="MPKTVIIGAGSGFGTRLSIDILAYPELREGTIALVDIDPEQLAP
                     ATAFVEKVVALHGAPVNVIGTTDRREVLEGADFVVVAIAVGGPAYNGVPYYYEINIPS
                     KYGISQDVGDTLGPGGIFRALRTAPEMVAIARDMEELCPNALLLNYTNPMAMLCWILN
                     EASTVRNVGLCHSVQGTTAQLAGYMGKPLAEIGARVAGLNHMAWFLELTWKGQDAYPI
                     LRQAMEDPEIYAKDIVRFEIFRHFDYFVTESSRHMSEYVPYFRKNAQALEQFGIKQRQ
                     PEKEPARRGRFWEDSPLSRQLAGKEPINLKGSHEYASHIIHSVWTDTPGRINGNVRNS
                     GCISNLPPECCVEVPCLVDRMGIQPCCMGALPTQLAALNAANVAVQSLTVQAFLEKDR
                     RKAYHALLLDPVTAAICTPAQIKSLFDEMCAALRDWIPWLG"
     gene            4611..5075
                     /locus_tag="PLY56_04345"
     CDS             4611..5075
                     /locus_tag="PLY56_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20741.1"
                     /translation="MSYTIRLLAPKEEAPSVAAIERALRDEGFDVTITPVPPAGAWER
                     FELQMPDEDPVKVRRYLREGDENPVDEEVDGFLDELLDRDETPGLKQVMDALRRARQM
                     MVVEIPDSFPWSEERTVVDALVEHLADVTGAIIQADGEGFYDSAGDLLVPME"
     gene            5252..>6234
                     /locus_tag="PLY56_04350"
     CDS             5252..>6234
                     /locus_tag="PLY56_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20742.1"
                     /translation="MAEMERIILEGADSRAVNQTVTRLADADVLGQLLRREGRMAELA
                     TEGTDIKLDWVDGVEKALQHPEWLAAAEDEAAGIVAQGIRHIIWSGMGGSVQTIYALK
                     GMGYLDEPQMAIFPLDSTDPASLNRVVFDIAARERVTLAPERVTTEDVQRILSKTMMI
                     GVAMGMTSEEPITHLEWFDGLLNEYQIPDPGSHIQVMTLPDSYLDRFARARGSRMVPI
                     QLDGENHTGGRMSAPATRVFIRPAALMLMSRAMKQGGKPDGEHLRRLLSRAQALYGLS
                     HDLSDDERRALTLSDPWVRLGAFVAEAARAGRNKVFLILPPEWASVAPWIE"
BASE COUNT         1088 a         2050 c         2048 g         1048 t
ORIGIN      
        1 gcggccgtgg actgcgcgag cggaacggcc agcaccaggg ccagcccggc tatcgtgacc
       61 cacctcaact ccagtctccc ttcctgggct ccgccggatg caacccgatc cgcgcatcgt
      121 ggaggctgca cgccgcgtgc caatcaacct gccgccccgg gtgccgtgga tgaaggggcg
      181 agagggcgaa ggggtgaaat caatacctga aggagacgcc gctgggactg gcgagtgcag
      241 aggtgccgcc tggagccaga ccgggcggtg tgatctcctt gaatcagact gccggctcgc
      301 caggcgccgg caggtggcgc ggcgaggtga ttcgccggcc gcacttggct tgtcaggagg
      361 ggcagcacca tgcaggagat ggaataccgc accctgggac ggcatggcgt gaaggtctcg
      421 cgcctttgcc tgggcacgat gatgttcggc ggacccactc ccgaggagga ggcgatccgc
      481 atcatccacc gcgcgctcga cctggggatc aactttatcg atacggcgga tatgtacacc
      541 ggaggcgagt ctgagcgcat cacgggaaaa gcgattcggg ataggcgcga tcaggtggtc
      601 ctggcaacaa aggtccgcta tccggtcggc ggtggtccca acgatagcgg gctctcgcgc
      661 taccacatct tcaacgcggt ggatgcgagt ttgcgccgcc tgggaacgga ccacatcgac
      721 ctttactacc tgcacctgcc agactacgaa acgcccctcg aggagtcgct ctcggcgatg
      781 gatgacctgg tgcgccaggg gaaggtgcgc tatatcgggt gctccaactt ctacgcctgg
      841 cagatctgca agggcctctg gatcagtgac cggcgcggat gggcaccctt cgcgtgcgtt
      901 cagcccctct acaacatcgt gaatcgcgac gccgaggtgg aggtgttgcc gctttgcgcg
      961 gaggagggcc tgggcgtggt gacctacagc ccgctggcgc gcggcgtcct ctctggaaag
     1021 tatctccctg ggggcgatct tccgccggat tcgcgcgcgg cgcgcggcga taagcgcctg
     1081 cacgagacgg agctgcgtca ggagagcttc gaggtagcga cccgcctcaa agcactcgcc
     1141 agcgtcaagg gatgcacgct cacccaactc gcgctcgcgt gggtcatggc caatccgatt
     1201 gtcacctgcc ccatccttgg cccgcgcacg atggagcagt ttgaggataa catcggctgc
     1261 ctggacgtga cgatcacgcc ggaggatgag gcgaccatcg atgccttggt gcctcgggga
     1321 gagcacaccg gctacggctt caacgacccg caatacccgg tgcgtggccg gcccgtggcg
     1381 gcgtgagccc cgctatctgc caggcatcac gcggaatagg cggcgacggc gcgtgcctgg
     1441 cacgcctcgc ccagcagcct gagaatctgc tcgcttggga tcggcttcac gaggaccgca
     1501 gttgcgccac tggtcaggag acggcgcacc tgttcctcgg tggcgtgtgc cgtgaggatg
     1561 accacccggg ggggatccgg ctccgccagg atcaggcggc aggcatcctc cccgccgatc
     1621 ccgggcacgg cgaggtccag gaagacgatg gccggccggt agcaccgggt gaggtggacg
     1681 gcgctcatgc cgtcgctcgc cgtcgtgacc gcgtagccct ggtcaaccag gaggtcgcac
     1741 agcgttccgg tgaagagtgg ctcatcatcc gcgagcagga catccaccgt tcgagaggcg
     1801 cctggcgccc cgggttcggg actggccctc cacgtcccca tctgcatcct gatgcacccc
     1861 ctcccagaga atgcgcttgc tggggcatac ccattctcct ggcaacccaa acagtggaac
     1921 cccatcgcgt gtggcgtaag cctgcgcgcg caaggactgc cccacgcccg ctccggctgc
     1981 aagccgagaa ggagcgcgcg ctgatccggc gaactctcac accggttggt ggcgttcttc
     2041 acagaccgag ccaggggtga caatccccat gcaaccaatc gcccgtggag gatgcactcg
     2101 cccgtaaact ctcgcgacct gccagggccc cccgcaggag ccaatccgcc cggaactgac
     2161 ggcaatacga ttccctggca gatcgccttg aacttgggga cacgcaaaat gagagaagtg
     2221 gcctgtgtaa atggccagtt catgcctctc gccgaggcgt gcatatccgt caatgatcgc
     2281 ggttttctct ttggcgatag cgtctatgag gtcgctcgca cctatgaggg ccgcgtgtgg
     2341 gccctggatc gccatctgga gcggctgcgc cgcagcctcg agggcattca gattgagggc
     2401 gtcgatctga atgccgtgcg cgcgaacatg ctggaagcat accaacgctc cgggtttgac
     2461 aacgcggtga tctacgtgca gatcacccgc ggcgccggcc cgcgctcgca tgtgccggac
     2521 ggcccgatga aaccaaccat cctgatcacc gtccggctct gcaaaggcgc ccgtgaggag
     2581 caggttcgca acggcgtgga ggtgatcctg gtcgatgaga cgcgctgggc gcgcagagac
     2641 atcaagacga cgaacctgct gcccaacatt ctcgcgctgc aacaggcgca cgcgcggggc
     2701 gcccacgaag cggtcttcgt cacggatgac ggctgggtgc acgaggcatc ccatagcaat
     2761 ctcttcatcg tttctggggg cgtggtgcgc accccaccga aaggcccggc gctcctgccc
     2821 ggaatcacgc gcgacctcgt cctggaatgc gcccggcggg cgggaatccc cgccgaggag
     2881 ctgccggtca gccgcgaggc gctgctcagc gccgatgagg tcttcctcac cggcaccagc
     2941 tccgaggtcc tgggggtcgt tcagatcgat ggccagccgg tcgcgtccgg gaccgtcggc
     3001 ccgatcacgc gccggctcca cgcgctctat caggatcgcg tggcagctgg tgacgacgcc
     3061 tgacgcgata gagttagcct gaatgcggcg ctcgggtcga acccccccga gcgccgcaag
     3121 agccgcccac tcgccggcac tccctgctca tcccagccag gggatccagt cgcgcagggc
     3181 ggcacacatc tcgtcgaaca ggctcttgat ctgggccggc gtgcagatgg cggccgtcac
     3241 cggatcgagg agcaaggcat ggtaggcctt gcgacggtcc ttctccagga acgcctgcac
     3301 tgtcaggctc tgcactgcta cgttcgcggc gttcagagcg gcgagttgcg tcggaagcgc
     3361 gcccatgcag catggctgga tccccatgcg gtccaccagg cacggcactt ccacgcagca
     3421 ctccgggggc aggttggaga tgcacccgga gttgcgcacg ttgccgttga tccgcccggg
     3481 cgtgtccgtc cacaccgagt ggataatgtg cgaagcatac tcgtgcgaac ccttcagatt
     3541 gatcggctcc ttgccggcaa gctgccggct cagcggtgaa tcctcccaga agcggccccg
     3601 acgcgccggc tccttctctg gctgcctctg cttgatccca aactgctcca gcgcctgcgc
     3661 gttcttgcgg aagtagggca cgtattcgga catgtggcgc gacgactcgg tcacgaagta
     3721 gtcgaagtgg cggaagatct cgaagcgcac tatatccttg gcgtagatct ccgggtcttc
     3781 catcgcctgg cgcaggatcg ggtaggcatc ttgtcccttc caggtaagct ccaggaacca
     3841 cgccatgtgg ttgagaccgg cgactcgggc gccaatctcg gccagtggct tccccatgta
     3901 gccggccagt tgcgcggtgg tgccctgaac actgtggcag aggcccacgt tgcgcaccgt
     3961 gctcgcctcg ttcaggatcc agcagagcat cgccatcggg ttggtgtagt tgaggaggag
     4021 cgcgttgggg caaagctcct ccatatcgcg ggcaatcgcc accatctccg gcgcggtgcg
     4081 cagcgcgcgg aagatgccgc ccggaccgag ggtgtcgccc acgtcctggc tgatcccata
     4141 cttcgagggg atgttgatct cgtagtaata gggcacgccg ttgtaggccg gcccgcccac
     4201 ggcgattgcc acgacaacga agtcggcgcc ctctagcacc tcgcggcggt ccgttgtgcc
     4261 gatcacgttg acaggcgcgc cgtgcagcgc caccaccttc tccacaaacg ccgtggcggg
     4321 cgccagctgt tcgggatcga tatcgacgag ggcgatggtg ccctcgcgca actccgggta
     4381 ggccaggatg tcgatactca ggcgcgtgcc aaagccgctt ccggctccga tgatcaccgt
     4441 cttgggcata ctctctccac ctctctcccc ccaagcggcc agggggcggt gctcctgttc
     4501 ggcacgactg gcgagccaac ctcccgcgaa atgcaaaacc gggctggcct cgcctctcct
     4561 ccgtgccctg ccttgacagg cccccctccc ccctctataa taccgctgag atgagctaca
     4621 cgattcgcct gctggcgccc aaggaagagg ccccctccgt cgcagcgatc gaacgcgcgc
     4681 tccgcgatga gggttttgat gtcacgatca cgcccgtgcc ccctgctggc gcatgggagc
     4741 ggttcgaact ccagatgccg gatgaggacc cggtgaaggt acggcgctac ctccgcgaag
     4801 gggatgagaa ccccgtcgat gaggaagtag acgggttcct cgacgaattg ctcgaccgtg
     4861 acgagacgcc cgggctgaaa caggtgatgg acgcgctgcg tcgtgcccgg cagatgatgg
     4921 ttgtggagat ccccgattcc ttcccctgga gcgaggagcg taccgtcgtt gatgccctgg
     4981 tcgagcacct cgcggatgtc accggtgcaa tcatccaggc ggatggcgag ggcttctacg
     5041 acagcgctgg cgacctgctc gtgccgatgg agtaggaaca cgacaaccgg cttatcaacc
     5101 accgaaaaag catgggtgga tgccgctgca aggaagcgca ctgctcgcgc gctcctgggg
     5161 gagcgagagc gcctgacgcg cgacgcgcgc tcctcgcccg cgcgtgcgca tcgatgggcg
     5221 cagtcacttg cggctcatag gagagtggat catggctgag atggagcgga ttatcctgga
     5281 gggcgccgat agtcgcgccg tgaatcagac ggtgacgcgg ctcgcggacg ccgatgtgct
     5341 cgggcagttg ctgcgccgcg aaggacgcat ggccgagctc gccacggaag gcacggatat
     5401 caagctcgac tgggtagatg gcgtcgagaa ggcgctgcaa catcccgagt ggctggctgc
     5461 cgccgaggat gaagccgcgg gcatcgtcgc gcagggaatc cggcacatca tctggtccgg
     5521 gatgggcggc tcggtgcaga cgatctacgc gctgaaggga atgggctacc tggatgagcc
     5581 ccagatggcg atcttcccgc tcgatagcac cgacccggcc tcgctcaacc gcgtcgtctt
     5641 cgatatcgcc gcgcgcgagc gcgtgacact cgccccagag cgcgtgacca ccgaggacgt
     5701 gcagcgcatc ctctccaaga cgatgatgat cggcgtggcg atggggatga cctccgagga
     5761 gccgatcacc cacctcgagt ggttcgatgg cctgctgaac gaatatcaga ttcccgaccc
     5821 tggctcgcac atccaggtga tgacgctgcc ggactcctac cttgaccggt tcgcgcgcgc
     5881 ccgcggctcg cgcatggttc cgatccagct cgatggcgag aaccacaccg gcgggcgcat
     5941 gagcgctccg gccacgcgcg tcttcatccg tccggccgcc ctcatgctga tgagccgcgc
     6001 catgaagcaa ggcgggaagc ccgatggcga gcacctgcgc cggctcctct cgcgcgcgca
     6061 ggcgttgtat ggtctctcgc atgacctgag cgatgacgag cggcgcgcgc tgaccctaag
     6121 cgacccctgg gttcgcctgg gcgccttcgt cgccgaggcg gcacgcgccg gccgcaataa
     6181 ggtcttcctc attctcccgc ccgagtgggc ttcggtggcg ccgtggatcg agca
//
LOCUS       DAOSCV010000121         6207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8041_length_6207_cov_3.368823, whole genome shotgun sequence.
ACCESSION   DAOSCV010000121 DAOSCV010000000
VERSION     DAOSCV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_6207_cov_3.368823"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..347
                     /locus_tag="PLY56_04355"
     CDS             159..347
                     /locus_tag="PLY56_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HOJ20743.1"
                     /translation="MQRAMVERRQLPVTPVEPLRQGGDDDGPRRPNVPRPDTRRLLER
                     MRQVDPDQAKRYRQRSGE"
     gene            350..1138
                     /locus_tag="PLY56_04360"
     CDS             350..1138
                     /locus_tag="PLY56_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HOJ20744.1"
                     /translation="MAALEHCPSGDFLDLLRRREHPFAVGAPVRPVETGQPLIEAHGT
                     TVLAVKYAGGVLNVGDRRATSANAVMYDRAEKILEVDDTTLLAISGSYARALEVVRYL
                     RHAFHYYRRTQLQEMSLEGKLSEVSRVVAQNVASAMSGLGAFLPVISVYDATRGEGRI
                     FFYDGLGARFESEEWGAAGSGAERIRGAFDYILKTKGRFRDMSLDEALSEALVLLDIA
                     AEMDAMTGGYDKVLPSAKAVSAAGIEHLPEERLREAVSRIRRGA"
     gene            1138..1893
                     /locus_tag="PLY56_04365"
     CDS             1138..1893
                     /locus_tag="PLY56_04365"
                     /inference="COORDINATES: protein motif:HMM:NF012452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20745.1"
                     /translation="MLSPYDWNEAIRQRAEYIEERLAEGSPVVALSCREGILLATVRG
                     TQRKLFEIYDRLAFAALGQQADIEALRLNAIDFAHAEGFARSPDDVTIGRVVAALSPA
                     VKKAFGDPLAAPLVARSLFAEVGATPETDHFYALGYDGEFRAFRRLAVIAGSPTAEEQ
                     MRACLAEALGSGEIPSLREGRRHALRAWAAGWAARRSRGQKAEPAGEQDLDAVLGEAL
                     REGEVEMALLDRSTHRQCRFRVLREEETESERE"
     gene            2084..3571
                     /locus_tag="PLY56_04370"
     CDS             2084..3571
                     /locus_tag="PLY56_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HOJ20746.1"
                     /translation="MEHRVFGIETEFGCLVRDPAAGTAEEVVERVKDYAFYRKRIGVI
                     DLHARNYAFEPARSGGFLVNGGRLYVDAVGSHEEYATPECRSIFDAVCWDKAGQRLLQ
                     TLVNELGLRDAVAFFNNSVDHFGGHTFGCHENYLVLVDEVYQGEALARLIPFLVTRQI
                     FAGAGRVGGHRLNRHDFRNNVMRLGDHDVDTMWVDDFYGVEIDDSVNYQLSQRADHIV
                     NLVSSRVRFNRALINPRRDSFFDFSGLSRLHVLFGEANMSEYAAALKLGTTCLALSLI
                     EMRCVPDDVLLADPLATLREVSRDPDWRWLVVRDDGSTLSAVDLQRYYLAAAQRFLRG
                     LDPETDWVIREWEEVLDLLEKDPVALADRLDWVAKWRLLQTYMAAEGVGWDADVLHSL
                     DLEYHDIDPERSLYHALVDEGAMRRLVTDEEIDHAMDAPPANTRALGRARLVRELISR
                     RVRHYAIDWDAVYAGAEQLLELPDPFHTYEDEAGGFLAGLSKAGR"
     gene            complement(3621..5504)
                     /gene="dxs"
                     /locus_tag="PLY56_04375"
     CDS             complement(3621..5504)
                     /gene="dxs"
                     /locus_tag="PLY56_04375"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOJ20747.1"
                     /translation="MRLNEIRHPAQLRALSSRELEELAQEIRDLIVRTVTVTGGHLAS
                     SLGVVELTLALLTRFDPEKDRIVWDVGHQCYAFKILTGRRDRFHTLRQLGGISGFPLR
                     SESPCDAFGAGHASTSLSAALGFAKARDLKGEDHRVVAVIGDGALTGGMAWEAINNAG
                     VANTDLLVVLNDNEMSISRNVGALARHTSALRSLHLYQSIEENTERLLEQVPLTGQFL
                     VKAGRALKRGVTHWISPEHGTLFEALGFDYIGPIDGHNVGLLTDIFAHARNLKGPVLM
                     HVITKKGRGCDYAESDARRYHGVSGCAPENGEPKRSSLSYSQVFGATLCRLAEERPEI
                     VAITAAMPDGTGLTAFAKQFPERCFDVGIAEEHGATFAAGLAAGGIRPVVALYSSFLQ
                     RAYDQVIHDVCLQKLPVLLAIDRAGLVGQDGPTHHGIFDIAFLRHVPNLTVMAPRGGA
                     ELQAMLAYALQLDGPCAIRYPRDGDVGPLDAPVPPIEHGKAELLREGSDLAFIAPGDT
                     VARCLEAAALLEEKGLSAAVVNARFIKPLDAALLAELASRCQALVTVEEHVAAGGFGS
                     AVLEMLNAAGLPTDHVLSIALPDHFPTHGDSDALRARYGLDAPAIADRALRFLREKRA
                     PGE"
     gene            complement(5504..>6207)
                     /locus_tag="PLY56_04380"
     CDS             complement(5504..>6207)
                     /locus_tag="PLY56_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HOJ20748.1"
                     /translation="YEELLRGLKERYPIHLHALSPPEVLHLARVSRLSVREVLERLKA
                     AGLDSLPGGGAELLVDSVRERVSPRKARAEEWLGVMREAHYLGIPTTATMVYGFGESA
                     EERVEHLLKIRELQDETGGFTAFIAWSFQPEGTAIGGRRASGYDYLRVVATARLLLDN
                     IPHIQASWLTQGPKIGQAALHFGVDDFGSTVLEENVITAPGARFLIPLEELERLIRAA
                     GYEPRRRNTRYELTG"
BASE COUNT          974 a         2045 c         2183 g         1005 t
ORIGIN      
        1 tctggatctg ggcagcctgg tgacccggcc cggggagggg gatggagttc cggagcgcgt
       61 tgccgaggcg gtggctgcta tcgagcgcgc ggcgagcccg gccgatctgg ccggccttga
      121 gatggtcact tcgcgagctt attgaaagga gattcttcat gcaaagagcc atggtggaac
      181 ggcgccagct tcccgtgacg ccggtggagc cgttgcgaca gggcggtgac gacgacggcc
      241 cgcgccgccc gaacgtgccg cgcccagaca cccgccgcct cctggagcgg atgcgccagg
      301 tcgacccgga tcaggcgaag cgttaccgcc agcggagcgg agagtagcga tggccgcgct
      361 ggagcattgc ccttcgggcg atttcctgga cctcctgcgc cggcgggaac atcccttcgc
      421 ggtgggcgcg ccggtccgcc cggtcgagac cggccagcca ctcatcgagg cccacggcac
      481 cacggtcctc gccgtcaagt atgccggcgg ggtgctcaac gtgggcgacc ggcgggcgac
      541 ctcggcgaac gccgtcatgt atgaccgcgc ggagaagatc ctcgaggtgg acgacaccac
      601 gctcctggcg atctcgggct cttacgcccg ggcgctggag gtggtgcgct acctgcgcca
      661 tgcgttccac tactaccggc gcacccagct tcaggagatg agcctggagg ggaagctgtc
      721 ggaggtgtcg cgcgttgtag cccagaacgt ggcgagcgcg atgagcggcc tgggcgcgtt
      781 tctcccggtg atcagcgtgt atgacgccac ccggggcgag gggcggatct tcttctatga
      841 cggcctgggc gctcgcttcg aaagcgagga gtggggcgcc gccggctcgg gcgccgagcg
      901 catccgcggc gccttcgatt acatcctgaa gacgaagggc cggtttcgcg acatgagcct
      961 ggacgaggcg ctttccgagg cgctcgttct cctggatatc gccgcggaga tggatgccat
     1021 gaccggcggc tacgacaagg tgctgccctc ggcgaaggcg gtctcggcgg ctgggattga
     1081 gcacctgccg gaagagcgcc tgcgcgaggc ggtgagccgg atacggaggg gggcgtgatg
     1141 ctctcaccct atgattggaa tgaggcgatt cgccagcgcg ccgagtacat cgaggagcgc
     1201 ctggccgagg ggagccccgt cgtggccctc tcctgccgcg agggtattct cctggcgacg
     1261 gttcgcggca cgcagcgcaa gctgttcgag atctacgacc ggcttgcgtt tgccgcgctt
     1321 ggccagcagg ccgatatcga ggccctgcgc ctgaacgcca tcgacttcgc gcacgccgag
     1381 gggttcgcgc gcagccctga tgacgtcacc atcgggcgcg tcgtcgccgc gctcagcccg
     1441 gcggtgaaga aggcgtttgg cgacccgctc gcggcgccgc tcgtcgcgcg ttccctcttc
     1501 gccgaagtag gcgccacgcc cgagaccgat cacttttatg ccctcgggta cgatggcgag
     1561 tttcgggcgt tccggcgcct ggcggtgatc gcagggagcc cgaccgccga ggagcagatg
     1621 cgcgcctgcc tagccgaggc gctcggttcg ggcgagatcc cctccctgcg agagggccgt
     1681 cgccatgccc tgcgcgcctg ggcggccggc tgggcagccc gccgctcccg cgggcagaag
     1741 gccgagcccg ccggggagca ggacctggac gcggttctcg gagaggcgtt gcgcgagggc
     1801 gaggtggaga tggcgctcct ggatcggtcc acccatcgcc agtgccggtt ccgggtgctt
     1861 cgggaggagg aaaccgagag cgaacgggag tagcgcgggc tactgacctg aagtattcac
     1921 cagaatactt caaggaagac ttttgcccgg tggcacgccg ttgctgccac cagcgcccgg
     1981 tggcagagtg ttttcggggg cagggcccac gaacccggac tacgcctggc aaagagactt
     2041 cgatgaatga tgcgggctgg agggggtgtg agggaggcga ggtatggagc atcgcgtctt
     2101 cgggatcgag acggagttcg gctgcctggt gcgcgacccg gccgccggca cagcggagga
     2161 agtggtcgag cgggtcaaag actacgcgtt ctaccgcaag cggatcgggg tgatagacct
     2221 gcacgcgcgc aactacgcct tcgagccggc gcgctccggg ggcttcctgg tcaacggcgg
     2281 ccggctctac gtggatgccg tgggcagcca cgaggagtat gccaccccgg aatgccggag
     2341 catcttcgat gccgtctgct gggacaaagc cgggcagcgc ctccttcaga ctctggtgaa
     2401 cgagcttggc ctgcgcgacg ccgtcgcctt cttcaacaac agcgtggatc acttcggcgg
     2461 gcacaccttc ggctgccacg agaactacct ggtcttggtc gatgaagtct atcagggcga
     2521 ggcgctggcg cggctgatcc cgttcctcgt cacccgccag atcttcgccg gcgctggccg
     2581 ggtgggcggg caccgcctga accgccacga cttccggaac aacgtgatgc gcctgggcga
     2641 ccacgacgtc gacacgatgt gggtcgacga cttctacggc gtagagatcg acgactcggt
     2701 caactaccag ctctcgcagc gcgccgacca catcgtcaat ctcgtctcgt cgcgcgtgcg
     2761 cttcaaccgc gcgctcatca atccgaggcg cgactcgttc ttcgatttca gcgggctcag
     2821 ccggctccac gtcctcttcg gcgaggcgaa catgtccgaa tacgccgccg cgctgaagct
     2881 cggcaccacc tgccttgcgc tctcgctcat cgagatgcgc tgcgttccag acgacgtgct
     2941 tctcgccgac ccgctcgcca ccctccgtga agtctcgcgc gatcccgact ggcgctggct
     3001 cgtggtccgg gatgacggct ccaccctctc cgcggtcgac ctgcagcgct actacctggc
     3061 tgccgcgcag cgcttcctcc ggggccttga cccggagacc gactgggtga tccgcgagtg
     3121 ggaagaggtg ctggacctcc tcgagaagga cccggtcgcg ctggcggacc ggctcgattg
     3181 ggtggccaag tggcggctgc tgcagactta catggccgcg gagggcgtcg gttgggacgc
     3241 ggacgtgctc cacagcctcg atctagagta ccacgacatc gaccccgaac ggagcctcta
     3301 ccacgcgctc gtggatgagg gggcaatgcg tcggctcgtc acggacgagg agatcgacca
     3361 cgcgatggat gcgccgccgg ccaacacgcg ggcgctgggg cgcgcccggc tcgtgcgcga
     3421 gctcatctcg cggcgcgtgc gccactacgc catcgattgg gatgccgtct acgccggcgc
     3481 ggagcagctc ctggagctcc ccgatccctt ccacacctac gaggatgagg ccggggggtt
     3541 tctcgccggc ctttcgaaag caggccggta ggcccttacc cggcggtctc ctgccctctt
     3601 cagggcgcgt gggcgttatt ctattcgcca ggagcgcgct tttctcggag gaaccggagc
     3661 gcgcggtcag cgatggccgg ggcatccagg ccatagcgcg cgcgcagggc atccgagtcg
     3721 ccatgggtgg ggaagtggtc tggcaaagcg attgagagca cgtgatcggt gggcagcccg
     3781 gccgcgttta gcatctccag gacggcgctc ccgaagccgc cggcggccac gtgctcctcg
     3841 acggtgacga gggcctggca ccggctggcg agttccgcca ggagagccgc gtcgagcggc
     3901 ttgatgaacc gggcgttgac gaccgcggcg gagagcccct tctcctcaag gagggcggcg
     3961 gcttccaggc agcgcgccac cgtatcgccg ggcgcgatga acgccaagtc gcttccctcg
     4021 cgaaggagct cggctttgcc gtgctcaatg gggggcaccg gggcgtcgag ggggccgacg
     4081 tcgccatcgc gcgggtagcg gatcgcgcac ggcccgtcga gctgcaaggc gtaggcgagc
     4141 atcgcctgta gctcggcgcc gccgcggggg gccatcactg tcaggtttgg cacgtggcgc
     4201 aggaaggcga tatcgaagat gccgtgatgc gtgggcccat cctggcccac cagaccggcg
     4261 cggtcaatcg ccaggagaac gggcagcttc tgcaggcaga cgtcgtggat cacctggtca
     4321 taggcgcgct ggaggaagga ggagtagagc gcgaccaccg ggcggatccc cccggccgcc
     4381 agccccgcgg caaaggtggc gccgtgctcc tcggcgatcc ccacgtcgaa gcaccgctcc
     4441 gggaactgct tcgcgaaggc ggtgagcccg gtgccgtccg gcatggcggc ggtaatcgcg
     4501 acgatctcgg gccgctcctc cgccaggcgg cagagcgtgg cgccgaagac ctgtgagtaa
     4561 gagagcgagc ttcgcttcgg ctcgccattc tccggggcgc acccgctgac gccgtggtag
     4621 cgccgggcat cggactcggc atagtcgcac ccgcgcccct tcttggtaat gacgtgcatc
     4681 aggactggcc ccttcaggtt gcgcgcgtgc gcgaagatgt ccgtcaggag gccgacgttg
     4741 tgcccgtcaa tcgggccgat atagtcgaag ccgagggcct cgaagagggt gccatgctcc
     4801 ggtgagatcc agtgggtgac gccgcgtttc agcgcgcgcc cggctttgac caggaactgg
     4861 ccggtgagcg gcacctgctc caaaaggcgt tcggtgttct cctcgatgct ctggtagagg
     4921 tgaaggctcc ggagggcgct ggtgtgccgc gccagggcgc cgacgttgcg cgagatcgac
     4981 atctcgttgt cgttgagcac gacgaggaga tcggtgttgg cgacgccggc gttgttgatc
     5041 gcttcccagg ccatgccccc ggtgagcgcg ccatcgccga tgaccgccac gacgcggtga
     5101 tcctcgccct tgagatccct cgccttggca aacccgagcg ccgccgagag cgaagtgctg
     5161 gcgtgcccgg cgccaaacgc gtcgcacggg ctttccgagc gcaggggaaa gccgctgatc
     5221 ccacccagct ggcggagcgt gtggaaccgg tcgcgccttc cggtgaggat cttgaaggcg
     5281 tagcactggt ggcccacgtc ccagacgatg cggtctttct ccggatcgaa gcgcgtgagc
     5341 agcgccaggg tcagctcgac gacgccgagg ctcgatgcca ggtggccgcc cgtgacggtg
     5401 acggtgcgca cgatcagatc gcggatctcc tgggccagct cttctagctc gcgcgaggag
     5461 agggctctca attgtgccgg atgtcgtatc tcgttcagtc gcatcagcct gtcaattcat
     5521 agcgggtatt ccggcggcgg ggctcgtagc cggcagcccg aatgaggcgc tcgagctcct
     5581 ccagggggat cagaaagcgc gctcccgggg cggtgatgac attctcttcc agcacggtgc
     5641 tcccgaaatc atccactccg aagtggagcg cagcctgccc gatcttcggc ccctgggtga
     5701 gccaggacgc ctgaatgtgc gggatgttgt ccaggaggag ccgggcggtg gccaccacgc
     5761 gcaggtagtc ataaccggat gcgcgccgcc cgccgatcgc cgttccctcc ggctgaaagc
     5821 tccaggcgat gaacgcggtg aacccgcccg tctcgtcctg aagctcgcgg atcttcagca
     5881 ggtgctcaac gcgctcctcg gcgctctcgc caaagccata gaccatcgtc gccgtggtcg
     5941 gtatccccag gtagtgcgcc tcgcgcatca cgcccagcca ctcttccgcg cgcgccttgc
     6001 gcggcgagac gcgctcgcgc accgaatcca ccagcagctc cgcgcctcca ccagggagcg
     6061 agtcgagccc ggcggctttc agccgctcca gcacctcgcg aacactcagg cgcgacaccc
     6121 gcgccaggtg aagcacctcc gggggcgaga gcgcgtgcag gtggatcgga tagcgttcct
     6181 tcagcccgcg caagagctcc tcatacc
//
LOCUS       DAOSCV010000122         6204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8045_length_6204_cov_3.564319, whole genome shotgun sequence.
ACCESSION   DAOSCV010000122 DAOSCV010000000
VERSION     DAOSCV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_6204_cov_3.564319"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            682..4503
                     /locus_tag="PLY56_04385"
     CDS             682..4503
                     /locus_tag="PLY56_04385"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20749.1"
                     /translation="MNKVFWTTLCALLLGLALLPGAANAQDRDGDGLPDEIEVKLGTD
                     PDRSEGLQLLIDDRARGVGDTTIRADGKAPDIDKVFFAHAGGDRYVWKITFHDDYPAT
                     GTILHLYADLDDDRTTGRQDTEWARGVDVMYSFVDAKSDPRILNPAVRVSPAIPVRAI
                     VQGNAVYICDDVKMRVVDGKTRFRMHILSHLRNPATDSDTTEWIMVQVPLNPDRTPPE
                     LPYPRPEGFESITLPDFAQLAYSLWQERRTVRLRPRDAEVTGYTLLMSDDFDGQGEPG
                     ESVIWKCPRDGSYYIGLILRDATSALEGLDVWAGERKLGTIVGSSRAGREVLHYTERP
                     VRLSKGQPIRVATAKHSGPVRFHSVCLLAEKPKVPPLAISNLTAWHLPDEPGERPGRV
                     MIAFTTNRPATASARYTAIGAGAPPQEGTFDEGRGPVNNHYFMLPAELRAPGYRLEIR
                     CEEPRQEEYEAQSAKATYTVWRDPERHRAEHGIRTPARETPARIPLSVQEPTDRARAA
                     WPVTSGVPLPEGLLRDPQRCRLLDASGKSVPAQFQALAWWPASGTVKWLQVSFLASTT
                     PGQSTSYTLECGTPGSTTPNPIRVTASRPQAGEGVVGEAALPVTVNTGPLELTLDAGG
                     FAPFAQVTLNGKRVGSAAAGEGGFEIIDEKGTIYSSALAPPDQVLIEEQGPVRAVVFV
                     RGKLVNRNGEGFMRYLCRMHFHAGRPAVQVAFTLENDVMEPEMTRFQGLRARVPAQLA
                     GWRVACGTEDGSIPLRFGSRLLQDRDDRFTADGREGRRAAGWILASGAESALAIAVRD
                     FWQLYPKAIGADERGIVVDLLPELPHDVYAGASEDEINKLYFWCDEGRYKIRTGVRVT
                     TELAVDFAPEVQNGRYLSGAHWQHPLFAACTPEWYCASGAFGPMVPRAKGKFEVYERK
                     LDEAFAKFLARREMEREYGFLNYGDWFGERRWNWGNVEYDTQWALAANFARTGNLEML
                     WRAEQAERHNADVDTIHAAANPNLVGQVYTHCTGHTGGYFPETWEGMGGFNRGPRDSG
                     HTWAQGHFTLYALTGERRFLETGRKIADRFALSTTDFRYYAERNAGWPLIGLMGAYTV
                     DGNPAYLNAARLIADSVLWTQHPERGGWGHFLDPNECKHQPRCWGCKPFMTGVLLHGL
                     KMYDRAQPREEIKNAIRRNADFLWRETYVPEHAGFAYSECKTFITRGQNWTISLVGDG
                     LAYACLVDPEHKNRELLKQATAAFMHRSNISDFGKGFTQGTCFLPAMLHDLDALGLTE
                     IPPPAEEGPKP"
     gene            complement(4541..5101)
                     /locus_tag="PLY56_04390"
     CDS             complement(4541..5101)
                     /locus_tag="PLY56_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20750.1"
                     /translation="MQKETMERFPPQRVTLKNGKEVTIRPLLPEDGEALAAFYASVPQ
                     EDYRFYCPHPLDREHALANAAKAFSPLEVVLVLEPEPGRIGGYTWYRWKAPNAERSTF
                     GICVGRALQGTGAGRALMKRLMEIAAEVGPPIMCLTVQKANPRAVALYQSMGFRIVRE
                     QMRAPMADGLFPSEPEYAMERVNRPT"
     gene            5369..6007
                     /locus_tag="PLY56_04395"
     CDS             5369..6007
                     /locus_tag="PLY56_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ20751.1"
                     /translation="MSKRRGFTLIELLVVIAIIAILAAILFPVFARARENARKATCQS
                     NLKQLGIAFAMYLQDYDERGMPRDVGTAPNRVYWTRLVSPYLKNTGVLRCPSRSDSPP
                     KCFDIGDGSHYAYSYCFARNSAATINSPSELLIFVDWRASSVKWNATGCTGCTDGQTC
                     IQGRRWRPGVDTVPHMDGVNVCYYDGHVKYVPVATLNAAYLSVPVQKPWNNL"
BASE COUNT         1050 a         2042 c         2105 g         1007 t
ORIGIN      
        1 tcgcggtagt cggcgtagac atcgcagcca tactccctcg ccgagcgctg gcgccgttct
       61 tcgatgagat cgacggtcgc cacgatccgg aagtgctcgg ggttcttcga gaggtaggcg
      121 ccgtgaatgt cgcgcccgct gcgcccctgg ccgatgatcg ctacgttgag cgttgccatg
      181 atccgttccc ttctgcaaga gaggaggaga cgatgggaag gccctcggga tcccggaagc
      241 atctgggcca cacgccagca acggccggtc gcaggccccc ttgcctcttc ctctgcgttc
      301 ctcatgatgt gccgcccttc gcggctgcac ttcacccgga agggcgtcgc ctggatggca
      361 tcgttggccg gcggatgtcc gcacgccggg cgtgacccgc gtagggtgtc ggcatccgct
      421 gcctacaagc cttgccgatg gctacttcgc cggggaagag gggctctcct gttgcaagag
      481 gtgtgtcgga aagcatccgt gtcggttgag gcgggcccga cgggcgcgat ccggtagcgc
      541 agccgtttgg ccagacaagg aacctgggat gccatagaga atatgccgcc caaaaggcac
      601 ccactggccg gcaagagggg ctcctggcga gggcggaccg accgatgggg tggaaacggc
      661 gcggtgtcgg aggtgtgaga ggtgaacaag gtgttctgga caaccctatg tgcgcttctt
      721 ctggggctgg cgcttctgcc cggcgcggcc aatgcccagg accgggatgg cgacggcctc
      781 cccgacgaga ttgaggtgaa actgggcacg gacccggacc ggagcgaggg gctgcagctt
      841 ctcatcgacg atagagcgcg cggcgtcggc gacaccacca tccgcgcgga tggcaaggcg
      901 cccgatatcg acaaggtctt cttcgcgcac gcgggcggcg accgctatgt ctggaagatt
      961 accttccacg acgactaccc cgcgacgggc accatcctgc acctctatgc cgatctcgat
     1021 gacgaccgca cgaccggccg ccaggacacg gagtgggcgc gcggcgtgga cgtgatgtac
     1081 agcttcgtgg atgccaagag cgacccgcgt atcttgaacc ctgccgtgcg cgtctcgccg
     1141 gcaatccccg tgcgcgcgat tgttcagggc aacgccgtct acatctgcga tgacgtcaag
     1201 atgcgcgtgg tggacggaaa aacccgtttc cggatgcaca tcctgagcca cctgcgcaac
     1261 cccgcgaccg atagcgatac gacggagtgg atcatggtac aagtaccgct caatccagac
     1321 cgcactcctc ccgagctgcc gtatccccgc ccggaaggct tcgaatcgat caccctcccg
     1381 gatttcgccc agctggctta cagcctctgg caggagcggc gcacggtgcg cctccgcccg
     1441 cgcgacgcgg aggtgaccgg ctacaccctg ctgatgagcg atgacttcga tggccagggg
     1501 gaaccgggcg agagcgtgat ctggaagtgc ccgcgagacg gcagctatta catcgggctc
     1561 atcctgcgcg acgccacgtc tgccctcgag ggcctcgatg tgtgggcggg cgagcgcaag
     1621 ctgggcacca ttgtcggaag ctcgcgcgcg ggccgggagg tgctccacta caccgaacgc
     1681 ccggtccgcc tctccaaagg acagccgatc cgggtcgcga cggcgaaaca cagcgggccc
     1741 gtgcgcttcc atagcgtttg cctgctggcc gaaaaaccga aggtgccgcc gcttgccatc
     1801 agcaatctga cggcctggca ccttcccgac gagccgggag agcgccccgg gcgcgtgatg
     1861 atcgccttca cgacgaaccg cccggcgacg gcgagcgcgc gctacacggc catcggcgcc
     1921 ggcgcgcctc cccaggaggg aacgttcgac gagggccggg ggcccgtcaa caaccactac
     1981 ttcatgctcc cggctgagct gcgtgccccg ggctaccggc tggagatccg gtgcgaggag
     2041 ccacgccagg aagaatacga agctcagagc gccaaggcga cttataccgt ctggcgggat
     2101 cccgagcggc accgcgcgga gcacgggatc cgcacccccg cgagagagac gcccgcgcgg
     2161 attccgctga gcgtgcagga acccacggac cgggcgcgcg ccgcctggcc cgtgacttcc
     2221 ggcgtgccgc tgccggaggg gctcttgcgc gatccccagc ggtgccgcct cctcgatgcc
     2281 agcggcaaga gcgttcccgc gcagttccag gcgctcgcct ggtggccggc cagcggcacg
     2341 gtgaagtggc tgcaggtgag cttcctggca agcaccaccc caggccagag cacctcctat
     2401 accctggagt gcggcactcc tgggagcacc accccgaacc cgatccgagt gacagcctcg
     2461 cggccccagg caggcgaggg ggtggttggg gaagcggcgc tgcccgtcac cgtgaacacc
     2521 gggccgctgg agctgactct cgacgcgggc gggtttgccc cgttcgcgca ggtgaccttg
     2581 aatggaaagc gcgtggggag cgcggcggcg ggcgaaggcg gcttcgagat catcgacgag
     2641 aaaggcacga tctactcctc ggcgctcgcg cctccggacc aggtgctgat tgaggagcag
     2701 ggccccgtgc gcgcggtcgt cttcgtgcgc gggaagctcg tcaatcgcaa cggcgagggc
     2761 ttcatgcgct acctctgccg gatgcacttc cacgcgggcc ggcccgccgt gcaggtggcc
     2821 ttcaccctgg aaaacgacgt gatggagccg gagatgaccc ggttccaggg gcttcgcgcg
     2881 cgcgtgcctg cccaattggc tggctggcgc gtggcctgcg ggaccgagga tggctccatt
     2941 cccctccggt ttggcagccg gctgctgcag gaccgcgacg accgtttcac cgcggatggc
     3001 cgcgagggcc ggcgggctgc cgggtggatc ctcgcttcgg gcgcggagag cgcgctcgcc
     3061 attgcggtgc gcgacttctg gcagctctac ccgaaggcga ttggcgcgga cgagcgaggg
     3121 atcgtggtgg atctcctccc ggagcttccg cacgacgtgt atgctggcgc cagcgaggat
     3181 gagatcaaca agctctactt ctggtgcgac gaagggcgct acaagattcg caccggcgtg
     3241 cgcgtgacga ccgagctagc cgtggatttc gcccccgagg ttcaaaatgg ccggtatctt
     3301 tcgggcgcgc actggcaaca cccgcttttc gcggcgtgca cgcccgaatg gtactgcgcc
     3361 agcggcgcgt ttgggccgat ggtgccgcgc gcgaaaggca agttcgaggt ttacgagcgc
     3421 aagctggacg aggcgttcgc gaagttcctc gcgcgccgcg agatggagcg cgagtacggc
     3481 ttcctcaact acggcgactg gttcggcgag cgccgctgga actggggcaa cgtggagtac
     3541 gacacgcagt gggcgctggc ggccaacttc gcccgcaccg ggaacctgga gatgctctgg
     3601 cgcgccgagc aggccgagcg gcacaacgcg gatgtcgata cgattcacgc cgccgccaac
     3661 ccgaacctgg tgggccaggt ctatacccac tgcaccgggc atacgggtgg ctacttcccc
     3721 gagacgtggg aggggatggg cggtttcaac cgcgggccgc gcgacagcgg tcacacctgg
     3781 gcgcagggcc acttcactct ctatgcgctg acgggtgaga ggcggttcct ggagacaggg
     3841 cggaagatcg cggaccgctt cgccttgagc accacggatt tccgctacta tgccgagcgc
     3901 aacgctggct ggcccctgat cgggctgatg ggtgcctaca ccgtggatgg caacccggcc
     3961 tatctcaacg ccgcgcgcct catcgcggat tcggtactgt ggacgcagca tcccgagcgt
     4021 ggcggctggg gtcatttcct cgacccgaat gagtgcaagc accagccgcg ctgctgggga
     4081 tgcaagccgt tcatgaccgg cgtgctgttg catgggctga agatgtacga ccgggcgcag
     4141 ccgcgcgagg agatcaagaa cgccatccgg cgcaacgcag acttcctgtg gcgcgagacg
     4201 tacgttcccg agcacgcggg cttcgcctac tccgagtgta agactttcat cacgcgaggc
     4261 cagaactgga cgatcagcct cgtcggggat gggcttgcct acgcctgcct cgtcgacccg
     4321 gaacacaaga accgcgagct cctgaagcag gcgaccgcgg ccttcatgca ccggtcgaac
     4381 atcagcgact tcggcaaggg gttcacccag ggcacctgct tcctgccggc gatgctccac
     4441 gacctcgatg cccttgggct gacagagatt ccgccgcccg cggaggaggg gccaaagcca
     4501 tgagcgcgca atccacccgc ccccgcgggc tggcagagga ttacgttgga cgattcacgc
     4561 gctccatggc gtactccggc tccgagggga agaggccatc ggccattggc gcgcgcatct
     4621 gttcgcgcac gatccggaag cccatgcttt ggtagagcgc cacggcgcgc gggttggcct
     4681 tctgcaccgt caggcacatg atcggggggc cgacctcggc ggcgatctcc atcagccgct
     4741 tcatgagcgc gcgcccggcg cccgtgccct gaagcgcgcg cccgacgcag atgccgaagg
     4801 tgctccgctc ggcgttcggc gccttccagc ggtaccaggt gtagcccccg atgcggcccg
     4861 gctccggctc caggacgagg accacctcca gcgggctgaa cgccttcgcc gcgttggcca
     4921 gggcatgctc gcggtcgagc ggatgcgggc agtagaagcg gtagtcctcc tggggcacgc
     4981 tcgcatagaa cgcggccagg gcctcaccgt cttccgggag cagtggccgg atggtgacct
     5041 ccttgccgtt cttgagcgtt acgcgttggg gcggaaaccg ctccatcgtc tctttctgca
     5101 tgcttcctcc tccttgcccg ccggcgcggt ggtgtctcct ccgggcggct caccgcgcca
     5161 cttctgcggc gggcgcgcgc tttccttcac gagggagaca agggccgctt tagcataggc
     5221 gggtggtgcg ttgacgctcc ccgtgggggc tgctataatg ggaacgagcg gatcgcgctg
     5281 gaccccggtc tcacagagca cgggcatcgc gaagaccggc ttgctcctat cggcagcggc
     5341 ttcggcgccc gtctggaaag gaacgacgat gtcgaaaaga cgagggttta ccttgatcga
     5401 gctgctcgtg gttatcgcga ttatcgcgat cctcgcggcg atccttttcc ccgtgttcgc
     5461 gagggcgagg gagaacgcgc gcaaggcaac gtgccagagc aatctgaagc agctgggcat
     5521 agcgttcgcg atgtatctcc aagactatga cgagagggga atgccgcgtg acgtgggcac
     5581 agcgcccaac cgtgtctatt ggacacgcct ggtttccccc tacctaaaga acacgggcgt
     5641 cttgcgctgc ccctcccggt cggactcgcc tccaaagtgc ttcgatatcg gcgatggctc
     5701 gcactacgcc tacagctact gcttcgcccg caactcggct gccacgatca acagcccctc
     5761 ggagctcctg atttttgtgg actggcgggc gagctcggtg aagtggaatg ctaccggctg
     5821 caccggatgc acggacggcc agacgtgcat ccaggggagg cgctggcgcc cgggcgtcga
     5881 taccgttccg cacatggatg gcgtgaacgt ctgctactac gacggccacg tgaagtacgt
     5941 gccggtggcc accctgaacg cggcctacct gtcggtgccg gtccagaagc cctggaacaa
     6001 cctgtagcaa acagaagtgg gggctggcgg cgcgttcccc cgccagcccc ggacggtatg
     6061 ccgggaagcc tggccaggcc aggccgggtt accgcgatac caggtcgccg aagggcccca
     6121 gaagcgcgta cttgttcctc caggaagtgt acataatccc cacggcgttt ggagtgcgcc
     6181 gcagcgactc gagccacccc ttgc
//
LOCUS       DAOSCV010000123         6174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8092_length_6174_cov_3.581631, whole genome shotgun sequence.
ACCESSION   DAOSCV010000123 DAOSCV010000000
VERSION     DAOSCV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8092_length_6174_cov_3.581631"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLY56_04400"
     CDS             <1..207
                     /locus_tag="PLY56_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20752.1"
                     /translation="LVDTSGGDPEAGVVEYAVRQLGAERVLFGSDVPGRSYGVQLGKV
                     LGAQLTPEQRDLILFGNAERILGR"
     gene            204..965
                     /locus_tag="PLY56_04405"
     CDS             204..965
                     /locus_tag="PLY56_04405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20753.1"
                     /translation="MIDINAFAGPWPFRALPATLPADVATMLQEAGIARALVSPLEAL
                     FFEDPHIANERLAARLQPDGILLPCAAINPTLANWERSLRVSQEEIGARAVVLHPNYH
                     CYEPDSEPARALLAAAGKAGLPVLIQLRMQDLRAQHPLCKVPDVSAEKTLAAAESAPD
                     ARVILGGIRLAEVIAQASRIRALPNVWIELSQVEHVDGLRRAITAIGADRILLGTGAP
                     LFNVRSSLLKLEEGQLSDQERAAITRQNARELLAI"
     gene            1308..2375
                     /gene="amrS"
                     /locus_tag="PLY56_04410"
     CDS             1308..2375
                     /gene="amrS"
                     /locus_tag="PLY56_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013414033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOJ20754.1"
                     /translation="MKEALLYEKLGDGKVRCGVCRWRCVIPDGGQGVCATRLNRGGVL
                     HTLLYGRVASICADPIEKKPLYHFHPGTMVLSLGTVGCNFRCPGCQNYEISQVCAKDR
                     VAALHPISPEESVELALANACQGICWTYNEPAIWFEHTLESARLAKARGLYTVYVTNG
                     FISPEALDAIGPYLDAYRVDIKAFSREAYRKISGVARFEGILESAVRAREHWGMHVEC
                     VTNVTPTVNDDPAMLREIACWIRDALGPHTPWHITRFFPYRELSHLPPTPIDLLEQAH
                     AMAREEGLAYAYLGNVAGHGGEDTHCHACGARVIQRRGMGVTAIALEGGACHRCKTRI
                     PGRFEGAQVSSGLRHPVRPPT"
     gene            2450..3703
                     /locus_tag="PLY56_04415"
     CDS             2450..3703
                     /locus_tag="PLY56_04415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOJ20755.1"
                     /translation="MHLLIAGRWVVLATVVLSILLPIGLLVERGALVVLGLITAVYNS
                     GLSALLRAGRVRGLRPAVVVAIDLILVTLLNWFSGGVQSPFRGLYYVLIIVAAAIGDL
                     KGGLLTAAAATFAEAVMALIQPEVPGAEWQQHLSFAVSTVPYLFLTALGSGYLVRRLR
                     EQWERAEETEAQLAQVQRDLEIAREVQGALTHTAPLNVPGFELGAFTHTQYGIGGDLQ
                     DYVPIPEGIGVALADVVGHGLSAALLAARLAHLLDELGLGTPLHEVLESWNRTIYERT
                     PAEMYVTMAIIELRAADGRARYTVAGHPPPLHWRSATGEVRPLQVTGTALGALPEPQI
                     EVREMVLEPGDVLLIYTDGAVEARDDEGELLGIDGLSQLLARHVSLPPREMVNRIAAD
                     ILERCDVRDDMTLVAVMRRRTAPRR"
     gene            complement(3754..4320)
                     /locus_tag="PLY56_04420"
     CDS             complement(3754..4320)
                     /locus_tag="PLY56_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOJ20756.1"
                     /translation="MASRFRVLVIVSGIAALGLLAWAGRRPVRAGEERPAPVQTAVVV
                     LQPVGGSGVSGTIRFVQRDGVLEVTGTVTGLTPGPHGFHIHEYGDLTDTREGLSAGGH
                     YNPTGRPHGAPGEARRHVGDLGNIVADAQGVARINVRDRVIRLDGPYSIVGRAAVVHA
                     QADKFTQPVGDAGGRVAFGVVGIAAVDK"
     gene            complement(4536..5582)
                     /locus_tag="PLY56_04425"
     CDS             complement(4536..5582)
                     /locus_tag="PLY56_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ20757.1"
                     /translation="MSDTRQVLAIDGRGQFLVMEQPVPEVKPGQIRIRVRASLVSPGT
                     ELGGVPKLRENPNPDAAPRPFGYANAGDVVAVGEGVTRFKVGDRVACMGGGYALHATD
                     AVVPQNLSVHLPEGVSYADGSFAHLAATALHAVRRAEPEIGEHFLVVGLGIVGQLSAQ
                     FARLGGTHVMAWDRLPMRLEIARATGCDRPVNTAEEDGVEAAKEFTRGHGMDAAILAF
                     GGEGTEAFKQVVRTMKVAPDTHLMGRVVIVGGATITHTFAAGLGNLDVRSAARTGPGY
                     HDEAWEHGADYPSVYFSWNTQRNLEECIRFMDEGRIQVRPIVTHQVSLADAPKACDEL
                     VTNPGKTLGVVIEP"
     gene            complement(5823..>6174)
                     /locus_tag="PLY56_04430"
     CDS             complement(5823..>6174)
                     /locus_tag="PLY56_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20758.1"
                     /translation="FLHKSFPGWEFDVSKQRVSEDRAYKICWPFSLGAWRQTEEDPSG
                     FIVEGHSQIARWGVGGSYAVGGSRGCMRSCFNHLDARGKTECPYHNAPFVRRDRWILP
                     CRVPLEEERQGETE"
BASE COUNT         1027 a         2165 c         1965 g         1017 t
ORIGIN      
        1 ctggtcgaca cctcgggagg cgatccggag gcaggcgtgg tggagtacgc ggtgcgtcaa
       61 ctcggcgccg agcgcgtgct ctttggttcc gacgtgccgg ggcgctccta cggcgtgcaa
      121 ctgggcaagg tcctcggcgc ccaactcaca ccggagcagc gcgacctgat cctgttcggc
      181 aacgcggaaa ggatactcgg acgatgatcg atatcaacgc atttgccggc ccgtggccat
      241 tccgcgcgct cccggccact cttcccgctg atgtcgccac catgctccag gaggccggca
      301 tcgcccgggc actcgtttcg ccgctggagg cgctcttctt cgaggatcct cacatcgcca
      361 acgagcgcct ggccgcccgc ctgcaacccg atggtatcct gcttccctgc gccgcgatca
      421 acccgacgct cgctaactgg gagcggtcgt tgcgtgtctc gcaggaggag atcggcgcgc
      481 gcgcggtcgt gctccatccg aactaccact gctatgaacc cgactcggag ccggcacggg
      541 cgttgctggc cgccgccggc aaggcgggcc ttccggtgct gatccagctt cggatgcagg
      601 atcttcgcgc gcagcacccg ctctgcaaag tgccggatgt gagcgctgaa aaaaccctgg
      661 ccgcagccga gtcggcgccg gacgcacggg ttattctagg cggaatccgc ctggccgaag
      721 tgatcgccca ggctagccgc atccgcgcgc tccccaatgt ctggatcgag ctttcccagg
      781 tcgagcatgt cgatggcctc cggcgtgcga tcaccgcgat aggggcagac cggatcttgc
      841 tgggcaccgg cgctccgctt ttcaacgtcc gctcgtcgct cctcaagctc gaggaaggcc
      901 agctctccga ccaggagcgc gccgcgatca cgcgtcagaa cgcgcgcgag ctcctggcaa
      961 tctaaggcct atcgcgcccc aggcgcaata gatgcagcgg aagccccgtc gggtgatgga
     1021 cgtagaagcg ctgcgttctc tggggcgaac ccggtcttcc cttgccacat ccatgatacc
     1081 cggggcgaac ctttcgcgtt cgccccggtt ccgccagcag ggcgaacagt gaacgtttgt
     1141 cactccccga agccacggct gccctgtttt cagaagcccg agagaccttt atcaggcagt
     1201 agcgccggca tccagatcgg cgccaatacg cttgtcacca gcggcaaatg agggtacaat
     1261 agtcgtgtgt ctcgcctcgg cgatgcagtc attggcaagg agaagcggtg aaagaggcgc
     1321 tactctacga gaagctgggt gacgggaagg tccgctgcgg tgtctgccgg tggcgatgcg
     1381 tgatccccga cggcggccag ggcgtgtgtg caacgcgcct gaaccggggg ggcgtgttgc
     1441 atacgctcct gtatgggcgg gtggcctcca tctgcgcgga cccgatcgag aaaaagcccc
     1501 tctaccactt ccatccgggc acgatggtcc tctcgctcgg caccgtggga tgcaacttcc
     1561 gctgccccgg ctgccagaac tacgagatct cgcaggtttg tgccaaagac cgtgtcgccg
     1621 ctctccaccc gatttcgccg gaggagtcgg tcgagcttgc gctggcgaac gcgtgccagg
     1681 ggatctgctg gacctacaac gagccggcga tctggttcga gcacacgcta gagagcgcgc
     1741 gtctggcgaa agcccgcggc ctttatacgg tctacgtcac caacggcttc atctcgccgg
     1801 aggcgctcga tgccatcggg ccctatttgg atgcttaccg cgtggacatc aaggcgtttt
     1861 cgcgcgaggc ctaccggaag atttcgggcg tcgcccgctt cgaggggatc ctggaaagcg
     1921 ccgtgcgcgc ccgggagcac tggggcatgc acgtggagtg cgtcacaaac gtgaccccga
     1981 cggtgaacga cgacccggcc atgctgcgcg agatcgcctg ctggatccgc gacgccctcg
     2041 gcccgcacac tccgtggcac atcacgcgct tcttcccgta tcgcgagcta tcgcacctgc
     2101 cgccgacgcc catcgatcta ctggagcagg cgcacgccat ggcgcgcgag gagggcctgg
     2161 cctatgccta cctgggcaat gtggccggtc acggcggaga agacacccac tgccacgcct
     2221 gcggcgcgcg cgtgatccag cgccgtggta tgggggtaac ggcgatcgcc ctagagggag
     2281 gggcctgcca tcgctgcaaa acacgcatcc cggggcgatt cgagggggca caggtcagca
     2341 gcgggctccg gcatccggtg aggccaccga cgtgatgccg catggggcac cggtgactgg
     2401 ctcaccagag gcgcccgcga gagcgcctgc cccgcttgca ccagagttct tgcacctcct
     2461 gatcgccgga cgctgggtcg tgctcgccac ggtcgtcctc agcatcctcc tcccgatagg
     2521 cctcttggta gagcgcgggg cgctcgtcgt cctggggctg atcaccgcgg tctacaacag
     2581 cgggctgtcg gcgctgctgc gcgccggtcg ggtccggggg ctccgccccg ccgtcgtagt
     2641 cgccattgac ctcatcctgg tcaccctcct caattggttc tccgggggcg tgcagagccc
     2701 attccggggg ctctactacg tgctcatcat cgtggccgca gccatcggcg atctgaaagg
     2761 gggccttctt accgccgccg cagccacttt cgcggaggca gtcatggccc tcatccagcc
     2821 agaggtacca ggagccgaat ggcagcagca cctgagcttc gcggtcagca cggtgcccta
     2881 tctctttctg acagcgctcg ggtccggcta cctggtacgc cggctgcgcg agcaatggga
     2941 gcgcgcggag gagacagaag cgcaactcgc ccaggtccag cgagacctgg agatcgcgcg
     3001 ggaggtgcag ggcgccctca cccatacggc gccgctgaac gttcccggct ttgagctggg
     3061 cgcgttcacg cacacgcagt acggtatcgg cggggatctg caggattacg tgcccatccc
     3121 agaaggcatc ggcgtagccc tcgccgacgt cgtcgggcac ggcctttccg ccgcgctcct
     3181 cgccgcacgc ctggcgcacc ttttggacga actgggcctg ggcacgccac tccatgaggt
     3241 gctcgagtcg tggaaccgca ccatctacga acgcactccc gcggagatgt acgtgacgat
     3301 ggcgatcatc gagttgcgcg cggcggacgg ccgcgcgcgc tatacggtgg ccgggcatcc
     3361 ccctcccctg cactggcgga gcgcgacggg cgaggttcgc ccgcttcagg tgacaggcac
     3421 cgccctggga gcattgcccg agccgcaaat tgaggtgcgc gagatggtgc tcgagccggg
     3481 cgatgtgctc ctgatctaca cggatggcgc cgtcgaggca cgcgatgatg agggtgaatt
     3541 gctcgggatc gacggcctat cacagctgct ggcccggcat gtctcgctgc cccctcgcga
     3601 gatggtcaac cggatcgccg ccgatatcct tgaacgatgt gacgtgcgtg atgatatgac
     3661 actggtcgcg gtcatgcgcc ggcgcacggc tccccgtaga taacctgagg gccggaaagc
     3721 gccggcgcgt ggtagaaagc aggggcgcgg gggctacttg tccaccgcgg cgatccccac
     3781 cacaccgaag gcgacgcgcc cgccggcatc gcccacaggc tgggtgaact tatccgcctg
     3841 cgcatggacg accgccgcgc gtcccacaat cgaatagggg ccatcgagcc ggatcacccg
     3901 gtcgcgcacg ttgatgcgcg ccaccccttg ggcatccgca acaatgttcc cgaggtctcc
     3961 cacgtggcgt ctggcctctc ccggcgcgcc atgcggccgg cctgtcggat tgtagtgccc
     4021 gccagcagag aggccctccc gcgtatcggt cagatcgccg tactcatgaa tgtggaagcc
     4081 gtgcggtccg ggggtcaacc cggtcaccgt gccggtcacc tcaagcacgc cgtcacgctg
     4141 cacaaagcgg atagtacctg aaacgccact cccgcccacc ggctggagca ccacaacagc
     4201 cgtctgaacc ggagccggtc tctcttctcc cgcgcggacc gggcgtcgcc cagcccatgc
     4261 caacaggccg agcgcagcaa tccccgagac aatcacaaga acccggaatc ggcttgccat
     4321 tccactaccc cctctcgaaa cggtgatcca acgatgtagc tcccgaacga aagcggcgcg
     4381 ggcaagttgg ctaccaatcc gcgcaggggg ccggatgcgc gcttacgcgg cacccgaccc
     4441 gctgcgcctc ggtacgagca cggctcctct tccggcaagc acggcctgtt cacacccctg
     4501 ggggcattcc agaccgctgg acggacagcg cgccgctacg gctcgataac gacccccagc
     4561 gtcttgccgg ggttagtgac cagctcgtcg catgccttgg gggcatccgc cagcgacacc
     4621 tgatgggtga cgatgggacg cacctggatc cggccctcgt ccatgaagcg gatgcactct
     4681 tccaggttgc gctgcgtatt ccaggagaaa tagacggacg gatagtcagc gccatgctcc
     4741 caggcctcat cgtggtagcc cggtccggtc cgcgcggcgc tccgcacatc gagattcccc
     4801 agcccggcag cgaaggtgtg ggtgatcgtg gccccgccca caatcaccac gcggcccatc
     4861 agatgcgtat ccggcgccac cttcatcgtg cgcaccacct gcttgaacgc ctcggtgccc
     4921 tcgccgccaa aggcgaggat cgccgcgtcc atcccatgcc cgcgtgtgaa ctccttggcc
     4981 gcctcgacgc catcctcctc cgcggtattg accggacggt cgcatccagt ggcgcgagcg
     5041 atctccaggc gcatcgggag gcggtcccag gccatcacat gcgtgccgcc caggcgggcg
     5101 aactgcgcgg agagctgccc gacaatcccc aggccgacaa cgaggaagtg ctcgccaatc
     5161 tccggctcgg ctcggcgcac cgcgtgcaac gccgtcgccg ccagatgggc gaagctgccg
     5221 tcggcgtagc tcactccttc cggcaggtgg acggaaaggt tctgtggcac gacggcgtcc
     5281 gtcgcatgca gcgcgtagcc gccgcccatg cacgccaccc ggtctcccac tttgaagcgg
     5341 gtcacccctt cacccacggc gaccacgtcg ccggcgttgg catagccaaa ggggcgcggg
     5401 gcggcgtccg gattcgggtt ctcgcgcagt ttgggcacgc cgcccagctc cgtgccaggg
     5461 ctcaccaaag acgcacggac gcggattcga atctgccccg gcttcacttc cggcacgggc
     5521 tgctccatca ccagaaactg cccgcgccca tcaatcgcaa gcacttgacg tgtatcgctc
     5581 attagatccc tctcttttgc tcaaacgcag aactcggatg cccggttcca gcgcgccgga
     5641 ctcgcttcca tccgccctgc tcccgctgtt gcatccgggt gacgggtcga gacggcccct
     5701 gccgtgcccg ccacgtgtcc cgcgtgggat acatgctgag ttcggctaca accggccatc
     5761 tccctgcccg gcggtggcgc ggcgcctgcg aggcacgcgc ccgcccacca gacacccgcg
     5821 gcctactccg tctcgccctg gcgctcctcc tccagaggca cgcggcaggg gaggatccag
     5881 cggtcacgcc gcacaaaggg ggcgttgtgg taggggcact ccgtcttgcc ccgcgcatcc
     5941 aggtggttga agcagctccg catgcacccg cgcgaaccgc cgacggcata gctcccgccc
     6001 acgccccagc gcgcgatctg cgagtgtccc tcgacgatga agccggaggg atcctcctcg
     6061 gtctgccgcc aggctcccaa cgagaagggc cagcagatct tgtaggcgcg gtcctcgctc
     6121 acgcgctgct tcgagacatc gaactcccac ccgggaaagc tcttgtggag gaag
//
LOCUS       DAOSCV010000124         6170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8099_length_6170_cov_2.919869, whole genome shotgun sequence.
ACCESSION   DAOSCV010000124 DAOSCV010000000
VERSION     DAOSCV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_6170_cov_2.919869"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PLY56_04435"
     CDS             <1..883
                     /locus_tag="PLY56_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20759.1"
                     /translation="PKQYGDDILFVQVAPSNLPEPEADAVAKLRSAKKPVLTLRIPVE
                     EVEGVPAGLPEVAALFMGMKLTVAVFGYEHGIVFAGQPAVEAYKKYARDLREAPGDVP
                     FPTEVQETARFRSLFLYYESLIKKNLVSWGELQTTVGRLGGDMSDAADVYAAILVLAR
                     SAKRLGYLDFTFNGDLPDSAREVFARARHNLGAERLRVPAKIRTGPSDYHSTEQSETD
                     GPNELVSLRLVGMRHVAPVIGKYSDKFLLAQARGTWQAMEDADRWILMLTFPELSADV
                     IADLDAFFANVAKRLPA"
     gene            complement(955..1962)
                     /locus_tag="PLY56_04440"
     CDS             complement(955..1962)
                     /locus_tag="PLY56_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20760.1"
                     /translation="MADTIRVGLIGCGNNMRGAHLPRLVRFPEVRIAALCDPAPENIA
                     KMVEARPELRGVPVYDDYRRLLDKGGLDAVVISTPHTLHHRQIVDSLQCGLHVEVEKP
                     MVCSVEEARDVIAARDEAGKVVLVAYQRHYTGAWRYCREQILAGKLGPIHYVQAYQCQ
                     NWWQPGTPSAKAWRRRPELSGGGQLNDSGSHLVDILLWATGLEPEEVFAYQENRGAPV
                     DILSAVSIRFRGGAIGNLSIVGQSVRGFDEEVNLWGTEGTLQVLGTANQRVFHYTPEA
                     REVPPEERPDAGDPDRNFINAILGREEVQVPAECGLRVIALTEAIWRSAASGKAERCG
                     E"
     gene            complement(2196..5036)
                     /locus_tag="PLY56_04445"
     CDS             complement(2196..5036)
                     /locus_tag="PLY56_04445"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BpuSI family type II restriction endonuclease"
                     /protein_id="HOJ20761.1"
                     /translation="MPTILTPHLHEHNYYPPLKERLERALARLGLDNKGFHVVISPDA
                     ENQADLMVKQWTHDRLAIEVKKTREDVAAIRTWKQARGYVTNSTTKGLRYFCVTNGEL
                     LFIFRHEEGQRVAACMVADGRIDLGHFGPGGEADAVLDRWEEAITHLLHNLFIAGREY
                     ALDRTFAGLVEKYGEAHALLSLRLRHAVDTRMADPEWRAAYLEWCRTFSTQPEDAENR
                     AIAAEEAAHLLLHRILCYELFRAQLDLLDDSLKRKFRLPRVSLEPLTASAVGDAPILD
                     ALGEHFQALARINYAQVFAPDPVLDALPLDTEAEAVVRGFLEDVEAFPFDMSNFEEAR
                     RLFPTVFEALIPPEKRRRYGQVFTPRSLVELLCRVCIEDPEARVLDPACGTGAFLDGA
                     YDRLDQLALAMGKSAGHQALLERICGVEIGTFPLHLCAVRLALKHPAAPTRARLNHTD
                     FFATDPGSLFDGHPADVVLGNPPYIRQEALSEKEAIRARIRAALGEVSPGGRAYPYSP
                     LEADAYFYFVEYATAFLRDGGTAGWVLSDKFLVVETGRALKQFLLDHYHVRAVVLLAR
                     RAFSEAMVDNCLLILRKQPGGAAPDEPTIFLKVRRPLPTEEIVSLLANPVPGSNAYRR
                     LVMRPRASLDPAEKWTRYLADDGALAAVLRAPGVCPLPMVATYERGPDNGCAAFFFPS
                     AELIESFGIPEEFLVPAVERGNDLQELILRRTQCKSLLRVPPETDLSAPENAGLRAYI
                     EYASTPEFDTGYRHKGRYVPVPERHVVVTNAAGGPWWSFQRGSGEWHILVPRGYRTHY
                     KVVVNRARAHISTNFWGLRLRGAGRRGRELVLDIAFMAAFLNSILGQLQVEREARRYA
                     GFTKLERQELERLRILDPQQVPLEVRRQIYRLFLALDAAYGTPEYAGARQALDRALLR
                     YLGHEAAYDDLVDLLDELISEREASGSTPEAS"
     gene            complement(5078..>6170)
                     /locus_tag="PLY56_04450"
     CDS             complement(5078..>6170)
                     /locus_tag="PLY56_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20762.1"
                     /translation="RLYERDPDEITVEHWFPDVVLPYSPFGASRRGLKVSHLFSKRNL
                     AAASFLWHAIHELECDQTTRDLLRYTFTAALAQFTKMIRAKKEGTGWYVNAYRIPREH
                     RENNVWHSYENKFKAVLRMKRESNELLGRVSLSVEPPSRPEAACRIRCGSATDLSFIP
                     ANSVDYVFIDPPWAIKYFDLSVLWCAWMGFSLPFEQEIFPHETEFEEDMARALRELAR
                     VLRPGRSVTVNFATRYTEQINALRGAIERSGLEWVGTQAIQQDQLSFVQRQTDLALKA
                     NYLLTLQKSGEAAATEAVPVTAECVAGMIHALLNRTGPLSYDEIFQHLYPEIAQRPLH
                     CDILSVLRQSFQMVDERRPYAEPRWAVRA"
BASE COUNT         1062 a         2018 c         2074 g         1016 t
ORIGIN      
        1 gcccaagcag tacggcgatg acatcctgtt cgtccaggtt gccccgtcga acctgccaga
       61 accggaggcg gacgccgtgg cgaaactgcg ctcggcgaag aagccggtgc tcacgctgcg
      121 gattccggtt gaggaggtcg agggagtgcc tgcgggcctg cctgaggtgg cggcgctctt
      181 catggggatg aagctgaccg tggcggtctt cggctacgag cacgggatcg tcttcgccgg
      241 gcagccggcg gtggaggctt ataagaagta cgcgcgcgac ctgcgcgaag cccccggcga
      301 tgtgcccttc cccaccgagg ttcaggagac ggcgcgcttc cgctccctct tcctctacta
      361 cgagtcgctg atcaagaaga acctcgtctc gtggggcgag ctacagacga ccgtgggccg
      421 gctgggcggc gatatgagcg acgcggcgga tgtttacgcg gcgatccttg tcctggcgcg
      481 cagcgcgaag cgcctcggct atctcgactt caccttcaat ggcgacctgc ccgattccgc
      541 gcgcgaagtg ttcgcgcgcg cgcggcacaa cctgggcgcc gagcgcctgc gcgtgcccgc
      601 gaagatccgc accgggccca gcgactacca ctctaccgag cagagcgaga cggatggccc
      661 gaatgagctg gtgagcctgc gcctggttgg gatgcgccat gtcgcccccg tgatcgggaa
      721 gtacagcgac aagttcctgc tggcgcaggc gcgaggcacc tggcaggcga tggaggacgc
      781 agaccgctgg atcctgatgc tcaccttccc ggagctgagc gccgacgtga tcgccgatct
      841 ggatgccttc ttcgcgaatg tggccaagcg cctccccgcg taacatgatg gatgagccgg
      901 gcccggggga tcgcccgatc tccccgggcc cggccgggtg ttacccgaca cgtttcactc
      961 gccgcagcgt tcggctttcc cggatgcagc actacgccag atcgcctcgg tgagggcgat
     1021 gacgcgcagg ccacactccg cgggtacctg cacctcttcg cgcccgagga tggcgttgat
     1081 gaagttgcgg tcgggatcgc cggcatccgg gcgctcttcg ggcggcacct cacgcgcctc
     1141 cggggtatag tggaacaccc gctgattcgc cgtgcccagc acctgcagcg tcccttccgt
     1201 gccccacagg ttcacctctt catcgaaacc gcgcaccgac tgcccgacga tcgagaggtt
     1261 gccgatggcg ccgccacgga agcgaatgct gacggcggag agaatgtcca ccggggcacc
     1321 ccggttctcc tgataggcaa acacctcctc cggctccagg ccggtggccc agaggaggat
     1381 atcgactagg tgggaaccgg agtcgttcag ttggccgccc ccggagagct caggccggcg
     1441 ccgccacgcc ttggccgagg gcgtgccagg ctgccaccag ttctggcact ggtaggcctg
     1501 cacatagtga atcggcccca gcttcccggc gaggatctgc tcccggcagt agcgccaggc
     1561 gcccgtgtag tggcgctggt aggcgacgag cactaccttg cccgcctcgt cgcgggcggc
     1621 gatcacgtcg cgcgcctcct caacggagca gaccatcggt ttctccacct ccacgtgcag
     1681 gccacactgc agcgaatcca cgatctgccg gtggtgcagc gtgtgtggcg tggagatgac
     1741 caccgcatcc aggccccctt tgtccagcag gcgccggtaa tcgtcgtaga cgggcacccc
     1801 gcgcaactcg ggccgcgcct cgaccatctt ggcgatgttc tctggcgccg gatcgcatag
     1861 cgcggcgatg cgcacttccg ggaatcttac cagccggggc agatgggctc cccgcatgtt
     1921 gttcccgcac ccgatcaagc caacgcgtat cgtatctgcc atcttacgct tcctttcgcc
     1981 tcgctgttgg cacacgttcg ctggcgagcg gcgaacacct gcaggagaga tgcggcagat
     2041 ggtgcttccc taccccacgc cggcggcgat acttggatac cccggatgaa gggaacgaac
     2101 acggatggag aggcgcggct caagcggtgt cgcctattcc catctatcta tctgggctca
     2161 tctgtgttcg tctatagaag aagagccgcc ggaggtcagc tggcctcggg ggttgaaccg
     2221 ctcgcttcgc gttcggagat gagctcatcc aggagatcga ccaggtcatc gtaagccgct
     2281 tcgtggccca gatagcggag gagggcgcgg tcgagcgcct ggcgtgcgcc ggcatattcc
     2341 ggcgtgccat aggcggcgtc cagcgccagg aaaaggcggt agatctggcg gcggacttcg
     2401 aggggcacct gctggggatc gaggatgcgc agccgctcca gctcctggcg ctccagcttg
     2461 gtgaagccgg cgtagcgccg ggcctcgcgc tccacctgga gctgcccgag gatgctgttg
     2521 agaaacgccg ccatgaaagc gatgtcgagc accagctcgc gccctcgccg gccggcgccc
     2581 cgcaggcgca gcccccagaa gttggtcgag atgtgcgccc gtgcccggtt gaccaccacc
     2641 ttataatgcg tccggtagcc tcggggcacc agaatgtgcc actctcccga gccgcgctgg
     2701 aaggaccacc acgggccgcc cgcggcgttg gtcaccacca cgtgccgctc cggaacgggc
     2761 acataccggc ctttgtgccg gtaaccggta tcgaactccg gcgttgatgc atactcgatg
     2821 taggcgcgca ggccggcgtt ttccggcgcg ctcaggtccg tttcgggcgg cacgcgcagg
     2881 agcgacttgc actgcgtccg gcgcaagatc agctcttgca ggtcgtttcc gcgctctacc
     2941 gcgggcacga ggaactcctc cgggatgcca aagctctcga tcagctccgc gcttgggaag
     3001 aagaaggcgg cgcagccgtt gtccggaccg cgctcgtagg tggcgaccat cgggaggggg
     3061 cacactcctg gcgcgcggag aacggccgcg agagcgccat cgtcggcgag atagcgcgtc
     3121 cacttctcgg cggggtcgag agaggcccgg gggcgcatta ccaggcggcg gtaagcgttc
     3181 gagccaggca ccggattggc cagcagggag acgatctcct cggtgggcag cggccgacgc
     3241 accttcagaa agatggtcgg ctcgtcggga gccgcgccgc ccggttgctt gcgcaggatc
     3301 aggaggcagt tgtcgaccat cgcctcggaa aaggcgcggc gggcgaggag gaccacggcg
     3361 cgcacgtggt aatggtcgag cagaaactgc ttcagcgcgc gccccgtctc caccacgagg
     3421 aacttgtcgg agagcaccca gccggcggtg ccgccatcgc gcagaaatgc cgtggcatac
     3481 tccacgaagt agaaataggc atccgcctcc aacgggctgt agggataagc gcggccgcct
     3541 ggagagacct cgcccaaggc ggcccggatc cgcgcccgga tcgcctcctt ttccgagagc
     3601 gcctcctgcc ggatgtaggg cggattgccg agcacgacat cggccgggtg gccatcaaac
     3661 agcgagccgg ggtcggtcgc gaagaagtcg gtgtggttca ggcgcgcgcg agttggtgcg
     3721 gcggggtgct tcagcgccag ccggacggcg cagagatgga ggggaaaggt gccaatctcc
     3781 acgccgcaga tccgctccag gagggcttga tgcccggcgc tcttccccat agcgagcgcc
     3841 agctgatcga gacggtcgta cgcgccatcg agaaaggcgc cggtgccgca cgccgggtcc
     3901 agcacgcgcg cctccggatc ctcgatacag acacggcaga gcaactccac gagggaccgc
     3961 ggggtgaaga cctggccata gcgcctccgc ttttctggcg ggatcagcgc ctcgaagacg
     4021 gtcgggaaga gccggcgcgc ctcctcgaag ttgctcatat cgaagggaaa tgcctcaaca
     4081 tcctccagga agccgcgcac gacggcttcc gcctccgtat cgagagggag ggcatccagc
     4141 accggatctg gcgcgaagac ctgggcatag ttgatgcgcg ccagcgcctg gaagtgctct
     4201 cccagcgcgt ccaggatcgg ggcgtcgccc acggcactgg cggtgagcgg ctccagcgac
     4261 acgcgtggca gcctgaattt gcgcttcagg gaatcatcca ggaggtcgag ctgtgcgcgg
     4321 aacagctcgt agcagaggat gcggtgcagc aggaggtgcg cggcctcctc ggcggcgatg
     4381 gcgcggttct ccgcgtcctc cggctgagtg gagaaggtac ggcaccactc cagatacgcg
     4441 gcgcgccact cgggatcggc catgcgggta tcgacggcgt ggcgcagacg cagcgacaag
     4501 agcgcgtgcg cttcgccata cttctctacc aggccggcga aggtgcgatc gagggcatac
     4561 tcgcggccag cgatgaagag gttgtgcagg aggtgggtga tcgcctcttc ccaccggtcg
     4621 agcaccgcgt ccgcctcgcc cccggggccg aagtgcccga ggtcgattcg cccatccgcc
     4681 accatgcagg ccgcaacgcg ctgcccctcc tcatgccgga agatgaacaa cagctcgccg
     4741 ttggtgacac agaagtagcg cagccccttg gtcgtggagt tggtcacata gccgcgcgcc
     4801 tgcttccagg tgcgaatcgc cgccacatcc tcgcgcgtct tcttcacctc gatggccagg
     4861 cggtcatggg tccattgctt gaccatgagg tcggcctggt tctcggcatc gggcgagatg
     4921 accacgtgga agcccttgtt gtccaggccc aggcgtgcca gagcgcgctc cagccgctcc
     4981 ttcagcggcg ggtagtagtt atgctcatgc agatgcggcg tcaggatagt cggcatggca
     5041 ttccccgcta ggcggcggtg ctgcccgcgt cagaccgcta cgccctcaca gcccatcggg
     5101 gctccgcata tggccggcgc tcgtccacca tctgaaaact ctggcgcagc acgcttaaga
     5161 tgtcgcaatg cagcggccgc tgggcgatct ctggatagag atgctggaag atctcgtcgt
     5221 aggagagcgg tccggtgcgg ttgagaagcg cgtggatcat cccggccacg cactccgcgg
     5281 tcacaggcac agcctcggtg gcggcagcct cgcccgattt ctgcaaggtg agcaggtagt
     5341 tcgctttcag agccagatcg gtttggcgct gcacgaagga gagctgatcc tgctggatcg
     5401 cctgggtgcc cacccactcc aggccagagc gctcgatcgc cccgcgaagc gcgttgatct
     5461 gctccgtgta acgcgtcgcg aagttcaccg tcaccgaccg gcccgggcgc agcacgcgcg
     5521 ccagctcgcg caacgcacgc gccatatcct cctcaaactc cgtctcgtgg gggaagatct
     5581 cctgctcgaa gggaagcgag aagcccatcc acgcgcacca gagaacgctg agatcgaagt
     5641 acttgatcgc ccacggcggg tcgatgaaga cgtagtccac gctgtttgcc gggatgaacg
     5701 agagatcggt cgccgagccg cagcggatgc ggcaggcggc ctcggggcgc gacggcggct
     5761 ccaccgaaag cgagacgcgc cccaacagct cgttgctctc gcgcttcatg cgcaacaccg
     5821 ccttgaactt gttctcgtag gagtgccaca cgttgttctc gcggtgctcg cgcgggatcc
     5881 ggtaggcgtt gacgtaccag ccggtcccct ccttctttgc gcggatcatc ttggtgaact
     5941 gggccagcgc cgcggtaaag gtgtagcgca ggaggtcacg cgttgtctga tcgcattcca
     6001 gctcgtgaat cgcgtgccag agaaaggagg ccgcggccag attccgcttt gagaaaaggt
     6061 gggatacctt cagacctctg cgcgatgctc cgaacggcga gtagggcaag acgacgtcgg
     6121 ggaaccagtg ctccaccgtg atctcgtccg ggtcgcgctc gtagagccgg
//
LOCUS       DAOSCV010000125         6156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8123_length_6156_cov_4.026225, whole genome shotgun sequence.
ACCESSION   DAOSCV010000125 DAOSCV010000000
VERSION     DAOSCV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8123_length_6156_cov_4.026225"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLY56_04455"
     CDS             <1..605
                     /locus_tag="PLY56_04455"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ20763.1"
                     /translation="SWVAPPGSAILLSAVLRPAFPPGAVGRVGMAAALAVVDLLARAR
                     GLSARVKYPNDVLIAGKKVAGVLPEAYVAGGRLEWVVIGIGINLRREAVPPGLEAQAT
                     SMEEHAPLPERDGMIRSLLECLERRVEQANEDPGVLAAAWRACDTVLGQHVSLQAGLE
                     RFNGLAVDIDQDARLRLRLTDGSVRWLTPSEVTFRTSLSS"
     gene            complement(643..1092)
                     /locus_tag="PLY56_04460"
     CDS             complement(643..1092)
                     /locus_tag="PLY56_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HOJ20764.1"
                     /translation="MLARVILLLGLLSILDLSLLVLLGQRIGLWPTLGFILFTGVAGT
                     LLAKSQGLRVIRRIQGDLAAGRVPTYGLLDGLLILVGGALLLAPGILTDVAGLALMFP
                     FVRNRVKRRIREAIERGIRSGRWRVVRVGPAPGEEPPPSTPEWRRLR"
     gene            complement(1255..2040)
                     /gene="trpA"
                     /locus_tag="PLY56_04465"
     CDS             complement(1255..2040)
                     /gene="trpA"
                     /locus_tag="PLY56_04465"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ20765.1"
                     /translation="MTRIEEKFQELSKREGALICFLTAGDPSLSATEELILQVAESGA
                     DLIELGVPFSDPVADGPSIQASSLRALHNGTTLAGVLECVARVRRKIRVPLVLMSYYN
                     PILRYGLARFAADAVAVGVDGIIPSDLPPEEADEWLARARRQGLDTIFLLAPTSTDER
                     IRRVAEKASGFIYCVSRTGVTGAREALPDDLPGLVQRIRAHTEKPIAVGFGISTREQV
                     RQVTRLADGAVVGSALVNRIGRAGGATSEIAAFVRGLKAGTLR"
     gene            complement(2037..3260)
                     /gene="trpB"
                     /locus_tag="PLY56_04470"
     CDS             complement(2037..3260)
                     /gene="trpB"
                     /locus_tag="PLY56_04470"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ20766.1"
                     /translation="MAAIHQPVPDELGHFGRFGGRYVPETLIPALDELSQAWDAARRD
                     PEFQRELEYYLREYVGRPTRLTHAARLTAHCGGAKIYLKREDLCHTGSHKLNNALGQV
                     LLARRLGKKRVIAETGAGQHGVATATACALFGLECAVYMGEEDVARQALNVFRMRLLG
                     AEVVPVSSGTKTLKDAVNEALRDWVTNVRTTHYVIGSAIGPHPYPMLVRDLQSVIGRE
                     AKAQILEYEGRLPDAIVACVGGGSNSIGIFFPFIDTGTRLIGVEAAGRGLDTGAHAAT
                     LVAGSPGVLHGTHSYLLQDDDGQVLGTHSVSAGLDYPGVGPEHAYFKEHGKAEYVAVT
                     DSEALAAFQLLSELEGIIPALESAHAVAHALKIAPQMGKDAVLLVNLSGRGDKDVEQV
                     ARILAARNGKGKGEE"
     gene            complement(3326..4141)
                     /locus_tag="PLY56_04475"
     CDS             complement(3326..4141)
                     /locus_tag="PLY56_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOJ20767.1"
                     /translation="MRTRIKVCGITRVEDALAAVEAGADALGFVFAPSPRRVTPEKAR
                     EIISALPPLVTPTGVFVNSPAAEVAEIARATGLRAIQLHGDEPPDVLREVRARTGAWI
                     ARAFRVRGEETLAEIPRYLDYCDAILLDAHVPGAAGGTGQVFDWTLAARATALARIIH
                     QNNEKKSLCQAWREFVDTAPENTLPLGACSDNGISPDKGLLLKYSGEYFGLGRPMFLA
                     GGLHPGNVAEAVRQVRPYAVDVSSGVEAAPGVKDPERLRAFMAQVREADNLYR"
     gene            complement(4138..4920)
                     /gene="trpC"
                     /locus_tag="PLY56_04480"
     CDS             complement(4138..4920)
                     /gene="trpC"
                     /locus_tag="PLY56_04480"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOJ20768.1"
                     /translation="MFLDEILAWKREEVTRAKEETPLAELRACIDSLPPCRDFTSAVG
                     RPGQVNLIAEVKRASPSKGVIRTDFDPAAIATAYTEGGAAAISVLTDRRYFQGELGYL
                     SLVRKVTPLPVLRKEFIIDDYQLYEARAAGADAVLLIVAALPDGALRRLLDLAGELGL
                     HTLVEVHDRAELERACATGARVIGINNRDLRTFQTTLATTEALAPLVPADRVVVSESG
                     IFTRADVERVAAAGARAILVGEALMRAPQITPRVRELVGVAR"
     gene            complement(4953..5927)
                     /gene="trpD"
                     /locus_tag="PLY56_04485"
     CDS             complement(4953..5927)
                     /gene="trpD"
                     /locus_tag="PLY56_04485"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOJ20769.1"
                     /translation="MDLTRSEAAALMHAIMTGEATPAQTAALLTALKMKGERVDEITG
                     FTEAMRSHAIPVRPQRPDLVDTCGTGGDGTGTFNISTAAAFVVAGAGLGVAKHGNRSV
                     SSPCGSADVLEALGINLKMTPDRVAQAIDEIGVGFLFAQAFHPAMKNVASVRRELGIR
                     TIFNILGPLANPAGAPAQLLGVFHPALTEILARVLANLGIRRAFVVHGHGGLDEISTT
                     GETLVSEVRDGDVHSYRIDPTALGFRPPHPDELKGRDAKGNAEILLRVLDGEPGARRD
                     IVVLNAAVALVAGGKAPDLETGVALAQESIDSGAARAKLEALRRMSHQ"
BASE COUNT          936 a         2120 c         2093 g         1007 t
ORIGIN      
        1 gctcctgggt ggcccctccg ggctcggcga tccttctctc ggccgtgctg cgcccggcgt
       61 ttccgccggg tgcggtcggc cgggtcggga tggccgcggc gcttgcggtg gtggatctcc
      121 tcgcgcgcgc ccgcggcctc tcagcgcgcg tgaagtatcc gaatgacgtt ctgatcgctg
      181 ggaagaaggt ggccggggtg ctccccgagg cctacgtggc cggcgggcgc ctggagtggg
      241 tggtcatcgg aatcgggatc aacctgcggc gcgaggccgt tccccctggc ctggaagcac
      301 aggcgaccag catggaggag cacgcgccgc tccccgagcg cgatggcatg atccgttccc
      361 tgctggagtg cctggagcgg cgtgtcgagc aagcaaatga ggatccgggt gtgcttgcgg
      421 cggcctggcg ggcgtgtgac acggtgctcg gccagcacgt ttcgctccag gccgggctgg
      481 agcgcttcaa cggtctcgcc gtcgatatcg atcaggatgc gcgcctgcgc ctgcgcctca
      541 ccgatggttc cgtgcgctgg ctcactccct ccgaggtcac cttccggact tcgctgtcgt
      601 cttgaaccac cgcgcaggca tccgggtgct tcacccggtg ggctaccgga ggcggcgcca
      661 ctccggggta gagggcgggg gctcctcgcc cggggctggt ccgacacgca ccacacgcca
      721 gcgcccactc cggatcccgc gctcgattgc ctctcggatc cgccgtttca cccggttgcg
      781 aacaaacggg aacatcagcg cgagtccggc cacgtcggtc aggattcccg gagcgaggag
      841 gagcgcgccg cccaccagga tcagcaggcc gtcgagcagg ccgtacgtgg gcacgcgccc
      901 ggcggccaga tcaccctgga tccggcggat cacgcgtagg ccctggctct tcgcaagtaa
      961 cgtgcccgcg acacccgtga agaggatgaa cccgagcgtt ggccacagcc cgatgcgctg
     1021 cccaagcagg acgagcaggc tcaggtccaa gatcgacagc agcccaagga gcaggatcac
     1081 ccgcgcgagc atgacgccct cccgccgaaa gctggggaga cgagaggccg ggtggcatgc
     1141 accatcgcct gcgcgtccct aagaagcgcg ccacgcgccc gcggcttgtc cgcctgtgcc
     1201 tgttgccacg attcgaccac ggtgcctcct gctcgtctcc ccagtttcga gtccttatcg
     1261 gagcgtgccc gcttttagcc cgcgtacaaa cgccgcgatc tcggatgttg ccccgccagc
     1321 gcgcccgatc cggttcacca gggcgctccc gaccacggcg ccatcggcca gtcgcgtcac
     1381 ctggcggacc tgctcgcgag tcgagatccc gaagccaacg gcgatcggtt tctcggtatg
     1441 cgcgcgaatc cgctgcacca ggccgggcag gtcgtcgggg agcgcctcgc gcgcgccggt
     1501 gacccccgtg cgcgagacgc agtaaatgaa cccgctcgcc ttctccgcga cgcgccggat
     1561 ccgctcgtcc gtgctcgtcg gcgcgagtag gaagatcgta tccagtccct gacgtcgggc
     1621 gcgtgccagc cactcgtccg cctcctcagg gggcagatcc gaggggatga tgccatcgac
     1681 gccgaccgcc acggcatcgg cggcgaagcg tgccaggcca taccgcagga tcgggttgta
     1741 gtagctcatc agaaccaacg gcacccggat cttgcgccgt acccgtgcca cgcactccag
     1801 cacgcccgcg agcgtcgtgc cgttgtgcag ggcgcgcaat gaggacgcct ggatgctggg
     1861 accatcggcg acagggtcag agaagggaac gcccagctca atcagatccg cgccgctctc
     1921 cgccacctgg aggatcagtt cctcggtcgc ggaaagactc gggtcgcccg cggtgaggaa
     1981 gcagatgagt gcgccctctc gcttcgatag ttcctgaaac ttctcttcga tgcgcgtcat
     2041 tcctctccct tgcctttccc gttcctcgcc gccaggatgc gggcaacctg ctccacatcc
     2101 ttgtcgccgc gcccggagag gtttaccagg agcacggcgt ccttgcccat ctgaggggcg
     2161 atcttcagcg cgtgcgccac ggcgtgcgcg ctctccaatg cggggatgat cccttccagc
     2221 tcggagagaa gctggaacgc tgccagcgcc tccgagtcgg tgacggccac gtattcggcc
     2281 ttcccgtgct ccttgaagta ggcgtgttcc gggcccacgc cggggtagtc cagacccgcg
     2341 gagaccgagt gggtgcccag cacctggcca tcgtcatcct gcaggaggta ggaatgggtt
     2401 ccgtggagca cgcccggcga gccggccacc agcgtcgcgg cgtgggcccc ggtatcaagc
     2461 ccgcgcccag cggcctccac gccgatcagc cgggtgccag tgtcgatgaa cgggaagaag
     2521 atcccaatcg agttgctccc gccgccgacg caggcgacga tcgcgtccgg caggcggccc
     2581 tcatactcca ggatctgggc cttcgcctcg cgtccgatca ccgattgcag gtcgcgcacg
     2641 agcatcgggt aggggtgcgg gccaatcgcc gagccgatga cgtagtgcgt cgtgcgcacg
     2701 ttggtgaccc aatcgcggag cgcctcgttc accgcgtctt tgagcgtctt cgttccgctg
     2761 ctcacgggca ccacctcggc gcccaggagg cgcatccgga agacgttcag cgcctggcgc
     2821 gcgacgtctt cctcgcccat gtagaccgcg cactccagtc cgaagagggc gcacgccgtc
     2881 gccgtggcca cgccatgctg gccggccccg gtttcggcga tcacccgctt ctttcccaga
     2941 cggcgggcga ggaggacctg ccccagggcg ttgttcagct tgtgcgagcc ggtgtggcag
     3001 agatcctcgc gtttcagata gatctttgcc ccgccgcagt gcgcggtgag ccgggccgcg
     3061 tgggtgagcc gcgttggccg gccgacatac tcgcgcaggt agtactccag ctcgcgctgg
     3121 aactccggat cgcgacgggc cgcgtcccac gcctgggaga gctcgtcgag cgccggaatg
     3181 agtgtctccg gcacgtatct cccgccaaac cggccgaaat gcccgagctc gtcgggcacg
     3241 ggttgatgaa tcgccgccac gatgaccttc ctttcttctt tctacgcggc cctgccacga
     3301 cggcgcgggc cgattgcgcg tgtcactatc gatacaggtt atccgcctcg cgaacctggg
     3361 ccatgaaggc gcggaggcgc tcgggatcct tcacgccggg cgcggcttca acgccggacg
     3421 agacatccac ggcgtaaggg cggacctggc gcaccgcctc cgccacgttc ccggggtgga
     3481 gaccgcccgc cagaaacatg ggccggccta acccgaagta ttcaccagaa tacttcaaga
     3541 gaagtccctt gtctggtgat ataccgttgt cgctgcaagc gcccagtggc agggtgtttt
     3601 cgggggcagt gtccacgaac tcgcgccacg cctggcagag agacttcttt tcattattct
     3661 gatgaataat gcgggctaag gcggtggccc gcgccgcgag cgtccaatca aacacctggc
     3721 cggtgcctcc cgccgcgcct ggcacgtggg cgtcgagcag gatggcgtcg caataatcga
     3781 ggtagcgcgg gatctcggcg agtgtctcct cgcctcggac gcggaacgcg cgcgcgatcc
     3841 acgcgccggt tcgggcacgc acctcgcgga gcacatccgg cggctcgtcg ccatggagct
     3901 ggatggcgcg caagcccgtg gcgcgcgcaa tctccgctac ctcggcggcc ggagagttca
     3961 caaagacgcc ggtaggagtc accaacggcg gcagggccga gatgatctcg cgtgcctttt
     4021 cgggtgtcac gcgcctgggg cttggcgcga agacgaatcc gagcgcatcg gcgccggctt
     4081 cgactgccgc caaggcatcc tccacccggg tgatcccgca caccttgatc ctggttctca
     4141 tcgcgccact ccaaccagct cgcgcactcg cggcgtgatc tgcggcgcgc gcatcagcgc
     4201 ctcgccgacg agaatggcgc gcgcacccgc ggctgccacg cgctccacgt cggcgcgcgt
     4261 gaagatcccg ctttcgctca ccacgacgcg atccgcgggc accaggggcg ccagcgcctc
     4321 tgtcgtcgcc agcgtggtct ggaaggtgcg caggtcacgg ttgttgatcc cgatgacgcg
     4381 cgccccggta gcgcaggcgc gctccagctc ggcccggtcg tgtacctcca cgagcgtgtg
     4441 cagtccaagc tcgccggcca gatccaggag ccggcgcagc gcgccatccg ggagcgctgc
     4501 gacgatgagg aggaccgcgt ccgccccggc agcgcgcgcc tcatagagct ggtagtcgtc
     4561 gatgatgaac tccttgcgca gcaccgggag cggcgtgacc ttccggacaa gcgagaggta
     4621 gccaagctcc ccctggaagt agcgccggtc ggtgagcacg gagatggccg ccgcaccgcc
     4681 ctcggtgtag gctgtggcga ttgccgccgg gtcgaaatcg gtgcggatca cacctttgct
     4741 tggcgaggca cgcttcacct cggcgatcag gttcacctgc ccgggccggc ccacagcgct
     4801 cgtgaagtcg cggcatgggg gcaggctatc gatgcacgcg cgtagctctg cgagcggggt
     4861 ctcctctttc gcccgtgtca cctcttcccg cttccaggcg aggatctcat cgagaaacat
     4921 ggggcgttct cctatccggg tctcctgcgg ccttactggt ggctcatccg ccgcagggct
     4981 tccagcttgg cgcgggccgc gccggaatcg atcgactcct gggcgagagc gacgcctgtc
     5041 tccaggtcgg gcgctttgcc gcccgcgacc agggcgacgg cggcgttgag gacgacgata
     5101 tcgcgccggg cgcccggctc gccatccaga acacgcagta ggatctcggc gttgccttta
     5161 gcatccctgc cctttagctc gtcgggatgg ggcgggcgga agccgagcgc cgtggggtcg
     5221 atccggtagc tgtgcacgtc gccatcgcgc acctcggaaa cgagcgtctc gcccgtcgtg
     5281 ctgatctcat ccaagccgcc gtgcccatgc accacgaagg cacggcgaat gcctaggttg
     5341 gcaagcacgc gggcgaggat ctccgtgagc gcggggtgga agacgccaag aagctgggcc
     5401 ggcgcgccgg cgggattggc cagtggcccc aggatgttga agatcgtccg gatgccgagc
     5461 tcgcgccgga ccgaggcgac gttcttcatc gcggggtgaa acgcctgagc gaagagaaag
     5521 cccacgccaa tctcgtcgat cgcctgggcg acgcggtcgg gagtcatctt caggttgatt
     5581 cccaatgcct cgagcacgtc ggcgctcccg cagggcgagg agaccgatcg gtttccatgc
     5641 ttcgcgacgc cgaggccggc gcccgcgacc acgaacgccg ccgccgtgga gatgttgaac
     5701 gtgccggtgc cgtcgccgcc ggtgccgcag gtgtcgacca ggtcggggcg ctgcgggcgc
     5761 accgggatgg catgagatcg catcgcctct gtgaacccgg tgatctcatc cacgcgctcg
     5821 cccttcatct tgagcgcggt gaggagcgcc gcggtctgcg cgggcgtggc ctcgccggtc
     5881 atgatggcgt gcatcagcgc cgccgcctcc gagcgggtga ggtccatgcc ggtggtggct
     5941 ttgcggacgg cgtcgacgac ggccggggag ccctgctcgg gagccgcccc ggtgggagcc
     6001 acaggcgatg ccgggcgtgc cgcgggaggg tgtacgcgct ccggtggccg ggcttctgca
     6061 ttcgcggggg caccggcgag gaagttccag aggagctcct tgccgtgttg cgtcaggatc
     6121 gcctccgggt ggaactgcac cccctcgatc ggatag
//
LOCUS       DAOSCV010000126         6143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8141_length_6143_cov_3.352825, whole genome shotgun sequence.
ACCESSION   DAOSCV010000126 DAOSCV010000000
VERSION     DAOSCV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_6143_cov_3.352825"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLY56_04490"
     CDS             <1..532
                     /locus_tag="PLY56_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ20770.1"
                     /translation="LGTLRDEGADPAGKRVVVLGAGGSARSVVVSLARAGASAITIAN
                     RTLSRAEVLAELGNEWGCKTTRGVSLDAGGLRECIREAEILVNATSVGMHPRADAPLL
                     VPEDGLHPNLFVYDLIYNPPVTRLMAAAQRRGARAANGLRMLVLQGAASFRIWTGQQP
                     PVAVMERALRQALSQP"
     gene            749..2533
                     /locus_tag="PLY56_04495"
     CDS             749..2533
                     /locus_tag="PLY56_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ20771.1"
                     /translation="MAKKNVGERLVELKVISPEQYRQAQDVWVNSGRRGDIGRVIVEQ
                     GFAKEKDVAKAQAELAGVPFVDLAQFKVNEEAVRLVRKELAQRYDLVAVQKLGQKLFV
                     AMKDPSNVIALDDLKLVTGMQVVAALATPGDIEEAIARYYGSDEAETSESQAAAPAAA
                     PPVSVASANPLADGQAALKDLAGLGANQEVEELPAGADEDANKAPIIRVVNAIIQQAI
                     KDGSSDIHIEPGARFTRVRYRIDGVLHEVMQLPKYIQPPLLSRIKIMSDLNIAERRIP
                     QDGRIGITYGGKDYDLRVSILPTVFGEKAVMRILDKSSVMIGLNKLGFYPETLAQLES
                     VIVQPNGMILSTGPTGSGKTTTQYSILNKINSVEKNIITIEDPVEYQLPGISQVQVNR
                     KAGLTFAAGLRSFLRQDPDIIMVGEIRDLETAETAIEASLTGHLVLSTLHTNDAPSAV
                     TRLVDMGVEPFLISASLIGVLAQRLARRICPECKVPYTPPPDAVARLGVKQDPDGPPI
                     TFYRGKGCENCRHTGYKGRTGIHEMLLMNDEINDLVVRRAPLSEIREAGRANGMKLLR
                     EDGMMKALEGITTVEEVLRVVFTAGHTS"
     gene            3062..4150
                     /locus_tag="PLY56_04500"
     CDS             3062..4150
                     /locus_tag="PLY56_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008313891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ20772.1"
                     /translation="MHLDDLLRKAVEKDASDMHLVVGVPPILRVDGQLTPMNFARVTP
                     QDSQRIIYDIMTDEQIQRFESELELDFSYSLAKLARFRVNVYKDNGTVAAAFRKIPQK
                     IPTMRDLGLPLVLEELTKKRRGLVLVTGPTGSGKSTTLAAMINQINSERSEHIITIED
                     PIEYLHTHKCSIINQREVGQDTRSFNNALRAALREDPDVVLVGEMRDLETIQIAITTA
                     ETGHLVFATLHTNNAAQTVDRMVDVFPPGQQEQIRFQLSNNLEAVICQQLLPRAGMPG
                     RVCAMEIMICTPAIRNLIRENKAHQIPSAIQTGANVGMQSMDQHLRDLYQKGIITYEL
                     ALERAMNPDELKKMLAGREGQPAGGRPQ"
     gene            4262..5557
                     /locus_tag="PLY56_04505"
     CDS             4262..5557
                     /locus_tag="PLY56_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ20773.1"
                     /translation="MPTFEYTVRGQDGKLMKGTSEAENERTLAARLQEQGYLPVKVKK
                     VRGAKAARAASAPGGKAEVTLVNKKKKEGGGFVFGGVKLKDLSIFARQFATMINAGVS
                     LVRCLSVLREQTASPRLKLVVADLLTEVEAGSTLSSAMHKHPKVFSNLAVGLVRAGEV
                     GGVLDETLERLAGFMESDMELRRKVKSAMTYPTLVMIAAVGIVLFLVTFILPKFMAMF
                     KDLGVQEMPAMTAFLMSASDFLTKGFPTRQIIFVAVGTALFIGWKRFTRTKVGKRFWD
                     MCKLRVPVFGALNHKISVARFSRTLGTLLVSGVPILQAMETVAGTVDNEIIADAIMKA
                     RAAVREGESIGPPLQKSKLFPPMVVHMVSIGEETGALDGMLAKVADFYEGEVDAALES
                     LTASLEPVMIVGLGAIVGFIVIAMFMPLISVMSSLSGGE"
     gene            5598..>6143
                     /locus_tag="PLY56_04510"
     CDS             5598..>6143
                     /locus_tag="PLY56_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOJ20774.1"
                     /translation="MGALFVLGLTVGSFVNVCIYRMPLDQSVVRPPSRCPACNTQLRA
                     KDLVPVLSFLIQGRKCRYCKAPISWRYAIVESLTGVAFATLFFVHGPSVALLFDAALV
                     AALIAICAIDLEHYIIPDELNLFLVVTGILRDVWGRVTTGSWSHAAHIAIPFTEWELT
                     LPSSVLGIVVGFTHFLAIGWLG"
BASE COUNT         1114 a         1835 c         2082 g         1112 t
ORIGIN      
        1 cctgggtacg ttgcgcgacg agggcgctga tccggcgggg aagcgcgtgg tggtgctggg
       61 cgccgggggc tccgcgcgtt ccgtcgtggt gagcctggcg cgcgccggtg cgtccgcgat
      121 caccatcgcc aaccggacgc tctctcgcgc cgaggtgctg gcggagctgg ggaacgagtg
      181 ggggtgcaag acgacacgag gggtgtcgct ggatgccggc gggcttcgcg agtgcatccg
      241 cgaagcggag atcctggtca atgccacctc cgtggggatg cacccgcgcg ccgacgcgcc
      301 cttgctggtg cccgaggatg gtctccatcc gaacctgttt gtctatgatc tgatctataa
      361 ccccccggtg acgcgcctga tggcggcggc gcagcgccgt ggcgcgcgcg cggcgaacgg
      421 cctgcgcatg ctggtgttgc agggggcggc ctcgttccgc atctggaccg gccagcagcc
      481 gccagtggcg gtcatggagc gcgctctaag acaggcgctc tctcagccgt gagggtgtcg
      541 aacgcgcctc gtgtgccttt ccgggcagaa cgagggcgtt gtgatcggcc ctgagaggcg
      601 ttgaccaacg ccttgatcct ttcttgcacc ctcggcaggg atatggtata atgtccttgt
      661 ggcacaccag gcgtctccat tcttatgcta tgccgggtgc ccaggcccgg cagacatgga
      721 ataaacaggg cggtgacagg caatggtaat ggccaagaag aacgtaggcg agcggctcgt
      781 cgagctcaaa gtgatctccc cggaacagta ccgacaggcc caggatgtct gggtgaactc
      841 cggtcgccgg ggcgatatcg gccgggtgat cgtggaacag ggcttcgcca aggagaagga
      901 tgttgccaag gcgcaggccg aattggcggg cgttcctttc gtggatctgg cccaattcaa
      961 ggtcaacgag gaggccgttc gcctcgtccg aaaggaactg gcgcagcgct acgacctggt
     1021 cgccgtgcag aagcttgggc agaagctctt cgtggcgatg aaagacccgt caaacgtcat
     1081 cgcgctcgat gaccttaagc tggtgacggg aatgcaggtg gtggccgcgc tggcgacgcc
     1141 gggtgacatt gaggaagcga ttgcgcgcta ctacggtagc gatgaggcgg agacgagcga
     1201 gagccaggcc gccgcgcctg cggcagcccc gccggtctcc gttgcctcgg ccaacccgct
     1261 ggcggatggg caggcggcac tcaaagacct ggctggtttg ggcgccaacc aggaagtgga
     1321 agagctgcct gcgggcgccg atgaggatgc caacaaggcg ccgatcatcc gcgtggtgaa
     1381 tgccatcatc cagcaggcga tcaaggacgg gagttcggat atccacatcg agccgggcgc
     1441 ccgcttcacg cgcgtccgct accgcatcga tggcgtcctc cacgaggtga tgcagcttcc
     1501 caagtacatc cagccaccgt tgctttcccg catcaagatt atgtccgacc tgaatatcgc
     1561 ggagcgccgc atccctcagg acggccgtat cgggattacc tacggtggca aggattacga
     1621 cctccgcgtt tcgattctcc cgacagtctt cggggagaag gcggtcatgc gtattctcga
     1681 taagagtagc gtcatgatcg gcctgaacaa gctcggcttc tacccggaga cgctggcgca
     1741 gctcgaatcg gtcatcgtcc agccaaacgg gatgatcctc tccaccggac cgacgggctc
     1801 gggaaagacg accacgcagt attccatttt gaacaagatc aactcggtgg agaagaacat
     1861 catcaccatc gaggacccgg ttgagtatca gctgccgggc atcagccagg tgcaggtgaa
     1921 ccgaaaggcg ggcctcacgt tcgctgccgg tctgcgctcc ttcctgcgcc aggacccgga
     1981 tatcatcatg gtaggtgaga tccgcgacct ggagacggct gagacggcaa ttgaggcttc
     2041 gttgacgggc cacctggtgc tctccacgct gcacaccaat gacgcgccct ccgcagtcac
     2101 gcgtctggtc gatatgggcg tggagccgtt cctgatctcg gcctcgctga ttggtgtgtt
     2161 ggctcagcgt cttgcgcgtc gcatctgccc tgaatgcaaa gtgccctaca cgcctccgcc
     2221 ggacgcagtg gcccgacttg gggtcaagca ggacccggat ggcccgccga tcaccttcta
     2281 ccgtggcaag gggtgcgaga attgtcggca caccggatat aagggccgca cgggcatcca
     2341 cgagatgctg ctgatgaacg acgagatcaa cgacctggtg gtgcgccgag cgccgctctc
     2401 ggagattcgc gaagctggcc gagctaacgg gatgaagctg ctccgcgagg acgggatgat
     2461 gaaggctctc gagggaatca ccaccgtcga agaggttctg cgcgtcgtct tcacggcggg
     2521 tcacactagc tgagcctgcg agaacccagg tcgatcgagg tgtgccccct gggcaaagag
     2581 agtgggccgc aagctcctcg cgcggaccgc ggagccgatc ccctggcggg cgacgcaggg
     2641 gagaagcggt ttcggattcg ccttgactgc cgcggcgggt gatcggcacg agccgccctg
     2701 tcgggatgtg tctctgacac tggtgcgccg aggccccggc gaccagaacc gatggccggg
     2761 tccgggcagc acaacccagt ggggggcccg gctatgcaac aggggctgtg ttggcctctg
     2821 ttcccctgtg gagtgaattc gcagggagcg tgggtgtacg ggtcaggtgc tcgcgcgcct
     2881 gacactgggg ggcggagcgc tcccttgttt ggtctctgga ttcacagctt cacggtatgg
     2941 ctgagcggta tagacggagt gaatgcgctc cccgccgcgc aggaaaggac tagccttgtc
     3001 tgacgtcagg aaccaggcgg ctcaggctcc tgccagccgc acgcagattc cccttgacag
     3061 cgtgcatctg gatgacctgc tgcgcaaggc ggtcgaaaaa gatgcctcgg acatgcacct
     3121 tgtcgtgggt gtgccgccca tccttcgggt ggacggtcag ctcacgccga tgaacttcgc
     3181 ccgcgtcacg ccccaggact cgcagcgaat catctacgac atcatgaccg acgagcagat
     3241 ccagaggttc gagtcagagc tcgagctgga cttctcgtat tcgctggcga agctggcgcg
     3301 cttccgcgtg aatgtctaca aggacaacgg cacggtcgca gcggcctttc ggaagatccc
     3361 ccagaagatc ccgacgatgc gtgatctggg cctgccgctc gtcctggagg agctgacgaa
     3421 gaagaggcgc gggttggtgc tggtgaccgg cccgacgggg tccggaaagt cgacgacgct
     3481 ggctgcgatg atcaaccaga tcaactccga gcgcagcgag cacatcatca ccatcgagga
     3541 ccccatcgag tacctgcaca cgcacaagtg cagcatcatc aaccagcgcg aggtggggca
     3601 ggatacgcgc agtttcaaca atgccctgcg tgccgccctg cgcgaggatc cggacgtggt
     3661 gcttgtcggt gagatgcgcg acctggagac gatccagatt gccatcacga cggcggagac
     3721 gggccacctg gtgttcgcca ccctgcacac gaataacgcg gcgcagaccg tggaccgcat
     3781 ggtggatgtc ttcccgcccg gccagcagga gcagatccgc ttccagcttt cgaacaacct
     3841 ggaggcggta atctgccagc agcttctccc gcgcgcgggg atgcccggcc gcgtgtgtgc
     3901 catggagatc atgatctgca ccccggccat tcgcaacctg atccgcgaaa acaaagcgca
     3961 ccagatcccc tcggcgatcc agactggcgc gaacgtgggg atgcagtcga tggaccagca
     4021 cctgcgcgat ctttaccaga aggggatcat cacctacgag ctggcgttgg agcgcgccat
     4081 gaacccggat gagctgaaga agatgctggc agggagagaa ggccagccgg ccgggggccg
     4141 cccgcagtga gcgtgaggtt ggaattgggc tcgcggggaa tccgcgagca ggccctggcc
     4201 cggtggctgg ggttgggtgg gtgtacgggc cggtaacccg gatcactgcc ggaggtggaa
     4261 catgccaaca ttcgagtaca cggtacgcgg ccaagacggc aagttgatga aaggcacgtc
     4321 ggaggccgag aacgagcgca cgctggcagc gcgcctccag gagcagggct acctccccgt
     4381 caaggttaag aaggtccgtg gcgcgaaggc ggcgcgtgct gcctccgctc ccggagggaa
     4441 ggccgaggtc accctcgtca acaagaagaa gaaagagggg ggcggctttg tcttcggcgg
     4501 cgtgaagctg aaggacctct cgatcttcgc gcgccagttc gccacaatga tcaacgcagg
     4561 cgtctcgttg gtgcgttgcc tctccgtcct gcgcgaacag accgcgagtc cgcgcctcaa
     4621 gcttgtggtg gccgacctcc tgacggaggt ggaagcaggc tccacgctct ccagcgcgat
     4681 gcacaagcac cccaaggtgt tctcgaacct ggctgtcggt ttggttcgcg ccggcgaggt
     4741 gggtggtgtg ctcgacgaga cgctcgagcg tctggctggc ttcatggaaa gcgacatgga
     4801 gctgcggcgc aaagtgaagt cggcgatgac ctacccgacg ctggtcatga tcgccgctgt
     4861 gggcatcgtc ctcttcctgg tgaccttcat ccttcccaag ttcatggcga tgttcaagga
     4921 tctgggcgtg caggagatgc cggcgatgac ggcgttcctg atgagcgcga gcgatttcct
     4981 taccaaaggc tttccaacgc gccagatcat cttcgtcgcc gttgggacgg cgctcttcat
     5041 cggctggaaa cgcttcacgc gcacgaaggt gggcaagcgt ttctgggata tgtgtaaact
     5101 gagggtcccc gtattcggtg cgctaaacca caagatcagc gtggcacgct tctcccgtac
     5161 gttgggcacg ctcttagtct cgggcgtgcc catcctccag gcgatggaaa cggtggcggg
     5221 aaccgtcgat aacgagatca tcgccgacgc catcatgaag gcgcgcgccg cggtccgcga
     5281 aggtgagtcg atcgggccgc cgctgcagaa gagcaagctc ttcccgccga tggtggtgca
     5341 catggtctcc atcggcgagg agacgggcgc cctcgatggc atgttggcga aggtggccga
     5401 cttttacgaa ggggaagtgg acgcggcgct ggagtcgttg acggcgtcac tcgagccggt
     5461 gatgattgtc ggcctcggtg cgatcgtcgg cttcatcgtg atcgcgatgt tcatgccgct
     5521 catttcggtg atgagcagcc tgagcggcgg ggagtaggcc gatcggagag ctcgcggtac
     5581 taccggcgtg gttttgggtg ggagcgctgt ttgtcctggg gctgacggta ggcagcttcg
     5641 tcaacgtctg catctaccgg atgcccctgg accagtcggt ggtacgaccg ccctcgcggt
     5701 gcccggcgtg caacacgcag ttgcgagcca aagacctggt gccggtgctg agcttcctga
     5761 ttcaggggcg caagtgccgc tattgcaagg cgccgatctc atggcgctac gccattgtgg
     5821 agagcctcac cggggtagcg ttcgccacgc tcttcttcgt gcatgggcct agcgtggcgc
     5881 tcctcttcga cgcggcactc gtcgcggcgc tgatcgccat ctgcgccatc gacctggagc
     5941 actatatcat cccggacgag ctgaacctct tcctggtggt gacggggatc ctgcgcgatg
     6001 tgtgggggcg ggtgacgacc gggagttgga gccacgcggc gcacatcgcc attcccttca
     6061 cggagtggga gctgaccctc ccttcgtcgg ttctcggtat cgtcgtgggc ttcacgcact
     6121 ttctcgcgat cgggtggctg ggg
//
LOCUS       DAOSCV010000127         6121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8179_length_6121_cov_3.622816, whole genome shotgun sequence.
ACCESSION   DAOSCV010000127 DAOSCV010000000
VERSION     DAOSCV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_6121_cov_3.622816"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PLY56_04515"
     CDS             complement(<1..1665)
                     /locus_tag="PLY56_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOJ20775.1"
                     /translation="MSRPSALPALLTRASRLLFLAALAGAPVVAGALDPHWGAWTPAA
                     GLLACSLLAAGALRLLARRLAGEPERLATPVDAPILLLLAATFFSVFPSVNRHASVLE
                     ALRVLTCIVAFVLALEMVHEALHAAESAPEGASTAQVGREQKRRGRQQRREKPAAAPA
                     EGWQVTRLDAPLIALWAALIAGAAWAGGRGAQEYLAALRDGQAAWRVFGGFSHPSTLI
                     AHLALTMPLMAGAALALRSRQDSLRATWPIPVVALGMALAASAFTGSRLGFLAVGLGF
                     LVFLLAFLAIRRSVTPVQAGLIAAGFVLAGALTVLAVPTLRYRAIAAFTGSEAFSMQF
                     RLTTWAAAWRCFLAHPLAGSGAGTFPAIMTRYSETSWAQTAHNHFAQTAAETGILGIA
                     GLLLLLVLWFITALRGMRARKPEGIAEEEGAREALRAALLAAALGGVAASTAYAAVDF
                     GWSIAATGWVLWAVMGAGMAAAGAARPARVRTQVERVALGLLVVLLLPAGALTLASWR
                     HQQAMLLKSSAPLAAVEAFTGALRVAPYSPETHRELGRLLGVLGIVS"
     gene            complement(1662..2582)
                     /locus_tag="PLY56_04520"
     CDS             complement(1662..2582)
                     /locus_tag="PLY56_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ20776.1"
                     /translation="MSAHRTPEPPDTRVDVVIAIINWNTRDDLALCLRSIYEGNTPPP
                     TFLPVVVDNASADGSADMVAEQFPQAVVVRNRENRGFAAANNQVLKASHSRYVFLLNP
                     DTQVHPGALRELTQFMDAHPDVGICGPLVLNPDGSVQYSARRFPTPLAGAFRNTFLGR
                     LFPNNRYTRDYLLSDWDHRSERDVDWLSGAAMMIRREVIDQIGLLDEQFFMYSEDVDY
                     CYRAGEAGWRVTFFPGACITHARAHSSDQAQIRMLVQFHRSMYRFWRKHYAPRSSWPM
                     RLLAPAGIVARAGLLLAKNLLDRARGRLGR"
     gene            complement(2579..3514)
                     /locus_tag="PLY56_04525"
     CDS             complement(2579..3514)
                     /locus_tag="PLY56_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ20777.1"
                     /translation="MNLSIVIVNWNTRSLLRACLRSLEAAGDLAGIEHEILVIDNASA
                     DGSAEMTRQEFPRVTLIANESNLGYAAATNQGFAAARGESILLLNPDTEVPPGALRVL
                     METLAAHPDAAAVAPRLVDPDGTVQRSVRGFPAPWALLAELSGLARLFPRCRCLGAYR
                     MRYWNHDDERRVDQPMASCLLIRREALEAVGPMDEEFPIFGNDADWCYRAWAAGWSIY
                     FTPRAEIVHHGGASTSQVRRAMFRETERGLTHFYRKHYRGRAGVLGYLLALAAIHAGT
                     AARILVFDARAALGKSPAAPPAREAVERKKERASP"
     gene            complement(3511..4203)
                     /locus_tag="PLY56_04530"
     CDS             complement(3511..4203)
                     /locus_tag="PLY56_04530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ20778.1"
                     /translation="MNTEATARPSGRVSAVLFDLDGTLVLTHIDFVAMRAAVQEVIAA
                     AGVPAEVVKGLDILTMATRAVEFVRARDGEAASQELIARAEDAMVEAEMLGLEGAGAA
                     PFAAEVLETLSAKGVGVAIVTRNCRRATEAVLARTGLACPVILTRDDVVRYKPDPAHL
                     LAALECLGATPEVAVMVGDHLMDIQAGRAAGMRTIGVLTPGRPDDYFASENPDLVVKD
                     LRSLLSFVLGGL"
     gene            complement(4248..4880)
                     /locus_tag="PLY56_04535"
     CDS             complement(4248..4880)
                     /locus_tag="PLY56_04535"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ20779.1"
                     /translation="MPAFEVAAKEMEVYVCARNAIEHAGIPYMVAGGYGVACYAHRRP
                     TKDIDFLILPADAERAITALETARFFIRRSDPRWLYQAQCDGIMVDLIFGSTTHRGIV
                     PITQEWLDHARPIAIGTETFLVAAPEELIGQKILAQHENRADWWDAEMILDRWHDQVD
                     WDRIVARTAIDPVKRLSFLLFIESRHSSAGWYPQSTLERTWREAAAMLGI"
     gene            5007..5471
                     /locus_tag="PLY56_04540"
     CDS             5007..5471
                     /locus_tag="PLY56_04540"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOJ20780.1"
                     /translation="MREKMAVIEQVICVEAPVEKVFDYIGRPENNKEWIPDVVESEKL
                     TEGPNRPGSRFRFVTRAPFGLRVSAEAEITAMDPPRMLEFQSTSGVEHRGRWELESEG
                     QITRVRFRLEYRLSGLGSVVMRAAGLNQFLSQHVQGSIDGLRRALENGKVRP"
     gene            complement(5478..>6121)
                     /locus_tag="PLY56_04545"
     CDS             complement(5478..>6121)
                     /locus_tag="PLY56_04545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOJ20781.1"
                     /translation="IRRAVISAVSAIGRIALERRLQQREQMQGLHSRLRELGEQLEVA
                     RQESALDPLTGLYNRRAFDDRLAQALTMHRVFGQSACLLMIDLDHFKQVNDTYGHVAG
                     DEVLRSVADCLTRTCRVKNDFVARFGGEEFAVILWNTSLKRGVVSAERILDSIRGLRV
                     AYEAHTIAITASIGIAEVQSGDTTTRWVERADRALYDAKASGRNCICEAPSPY"
BASE COUNT         1061 a         2034 c         2118 g          908 t
ORIGIN      
        1 tgaaacgatc ccgaggactc ccagcagccg gcccagctcg cggtgcgtct cgggcgagta
       61 gggggcgacc cggagcgcgc cggtgaacgc ttccaccgcg gcgagcggcg cgctgctctt
      121 caggagcatc gcctgctggt ggcgccagct tgcgagagtc agcgcgcccg cgggcaggag
      181 cagcaccaca aggagcccca gagccacccg ctcgacctgg gttcgcacgc gcgccgggcg
      241 cgccgcgccc gcggcggcca tgccggcgcc catcaccgcc cacaggaccc atccggtcgc
      301 cgcaatcgac cagccgaaat cgactgccgc gtaagccgtc gacgcggcca cgccaccgag
      361 cgccgcagcg aggagcgcgg cacgaagcgc ctctctggcg ccctcctctt ccgcgatgcc
      421 ctccggcttg cgggcgcgca tcccccggag cgccgtgata aaccatagaa cgagcaggag
      481 caacagcccg gcaataccca ggatgcccgt ctccgcggcg gtctgggcga agtggttgtg
      541 tgccgtctgg gcccagctcg tctccgagta gcgcgtcatg atcgccggga aggtgcctgc
      601 gccactgccc gcgagcgggt gcgcgagaaa gcagcgccat gccgcggccc acgtcgtgag
      661 ccggaactgc atcgagaacg cctcggagcc cgtgaacgcg gcgatggccc gatagcggag
      721 cgtcggcacc gcgagaaccg tgagcgcgcc agccagaacg aacccggcgg caatcagccc
      781 ggcctgaacg ggcgtcaccg agcgccggat ggcgaggaag gcgagcagga agacgaggaa
      841 gcccaggcca acggcgagga agccgaggcg cgagccggtg aatgcactgg ccgccagcgc
      901 catgccgagc gccaccaccg ggatcggcca ggtggcgcgc agcgagtcct gtcgcgaccg
      961 cagcgccagc gccgcgccgg ccatcagcgg catcgtcagc gccaggtggg caatcaacgt
     1021 gctcgggtgc gagaacccgc cgaagacgcg ccaggcggcc tggccatcgc gcagcgccgc
     1081 gagatactcc tgggcgccgc gcccgcccgc ccacgccgcg cccgcgatca acgcagccca
     1141 cagcgcgatg agcggagcat cgaggcgtgt cacctgccag ccctcagcgg gggctgccgc
     1201 cggcttttcc cggcgttgct ggcgcccgcg ccgcttctgc tcgcgcccca cctgcgctgt
     1261 gctcgcgccc tccggggcgg attccgcggc gtggagcgcc tcgtgtacca tctccaaagc
     1321 gaggacgaag gcgacgatgc acgtgaggac gcgcaacgct tccaggacgc tggcgtggcg
     1381 gttgacggaa ggaaacacgg agaagaacgt cgccgcgaga aggaggagaa tcggggcgtc
     1441 gacgggcgtg gcgaggcgct ccggctcgcc ggccaggcgc cgcgctagca ggcgcaacgc
     1501 gcccgcggcg agcagcgagc acgctagcag ccccgccgca ggagtccagg cgccccagtg
     1561 cgggtcgagc gctccggcca ccaccggcgc gcctgcgagc gcggcaagga agaggagccg
     1621 ggatgcccgt gtgaggagcg cgggcagggc ggacggcctg ctcatcgccc gagcctccca
     1681 cgggcgcggt cgagcaggtt cttggcgagc agcaggcccg cgcgcgcgac gatgccggcc
     1741 ggcgccagca ggcgcatcgg ccaggacgac cgcggggcat agtgcttccg ccaaaagcgg
     1801 tacatgctcc ggtggaactg cacgagcatg cggatttgtg cctggtcgct gctgtgcgcg
     1861 cgcgcgtgcg tgatgcacgc cccggggaag aaggtgacgc gccagccggc ctcaccggcc
     1921 cgatagcagt aatcgacatc ctcgctatac atgaagaact gctcgtcgag gaggccgatc
     1981 tggtcgatca cctcgcgccg gatcatcatc gccgctcccg agagccagtc gacatcgcgc
     2041 tccgagcggt ggtcccaatc gctcagcagg tagtcgcgcg tgtagcggtt gtttgggaag
     2101 agccggccca gaaaggtgtt gcggaaggcg cccgccaggg gcgtcgggaa gcggcgtgcc
     2161 gagtactgca cgctgccgtc gggattaagc acgagcgggc cgcagatccc cacgtcgggg
     2221 tgggcatcca tgaactgcgt gagctcgcgc agggcgcccg gatgcacctg cgtatccggg
     2281 ttcaggagga agacgtagcg gctgtgcgag gctttgagca cctgattgtt ggcagcggca
     2341 aagccccggt tctcgcgatt gcgcaccacc accgcctgcg ggaactgctc cgcaaccatg
     2401 tcggcgctgc catccgcgga ggcgttatcg accaccacgg ggagaaacgt gggcggtggg
     2461 gtgttcccct cgtagattga gcgcaggcag agcgccaggt catcgcgcgt attccagttg
     2521 atgatcgcga tgaccacatc cacgcgtgta tcgggcggtt ctggcgttcg gtgtgcgctc
     2581 aaggactggc cctctccttc tttctttcca ccgcttcgcg ggcaggcggc gccgcgggcg
     2641 acttgccgag cgccgcgcgc gcgtcaaaga cgaggatgcg cgctgccgtg ccggcgtgaa
     2701 tcgccgcgag cgcgagcaga tatcccagga cgcccgcgcg gccccgatag tgcttgcgat
     2761 agaagtgcgt caggccccgc tctgtctcgc ggaacatcgc ccgacgcacc tgcgacgtgc
     2821 tcgccccgcc atgatgcacg atctctgcgc gcggcgtgaa gtagatcgac cagccggcgg
     2881 cccaggcccg gtagcaccaa tcggcatcgt tgccgaagat cgggaactcc tcgtccatcg
     2941 gccccacggc ttccagggct tcgcgccgga tcagaaggca ggaggccatc ggttgatcga
     3001 ccctgcgctc gtcatcgtgg ttccaatagc gcatccggta ggcgcccagg cagcggcaac
     3061 gcgggaagag ccgggccaaa ccggagagct ccgcgagaag cgcccacggg gcagggaagc
     3121 cgcgcaccga gcgctgcacc gtgccatccg gatcgaccag gcggggggcg accgcagccg
     3181 cgtcgggatg agccgccagc gtctccatca gcacgcgaag cgctccaggg ggcacctcgg
     3241 tatccgggtt caacaggagg atgctctcgc ctcgggcggc tgcgaaaccc tggttggtcg
     3301 ccgccgcgta accgaggttg ctctcgttcg cgatcagggt gacccggggg aactcctgac
     3361 gggtcatctc cgcgctgccg tccgccgagg cgttgtcgat cacgaggatc tcgtgctcaa
     3421 tccctgccag atcgcccgcc gcttccagcg agcggaggca ggcgcgcagc agcgagcgcg
     3481 tattccagtt cacaatcacg atggagaggt tcacagtccc cctagaacga aggagagcag
     3541 actgcgcaga tccttgacga ccagatccgg gttctcggag gcaaagtagt catccggacg
     3601 gcccggggtc agcacgccga tggtgcgcat gccagcggca cggcccgctt ggatatccat
     3661 caggtggtcg cccaccatca cggcgacctc cggggtggcg cccaggcatt ccagggcggc
     3721 cagcaggtgg gccggatccg gtttgtagcg gaccacgtcg tcgcgcgtca ggatgaccgg
     3781 gcaggcgaga cccgtccgcg cgaggacggc ttcggtggcg cgccggcagt ttcgggtgac
     3841 gatggccacg cccacgccct tcgcgctcag cgtctccagt acctccgcgg cgaacggcgc
     3901 cgcccccgcc ccttcgagac cgagcatctc cgcctcgacc atcgcgtctt cggcgcgcgc
     3961 gataagctcc tgcgaggcgg cttccccatc gcgcgcgcgc acgaactcga ccgcccgcgt
     4021 tgccatggtg aggatatcca ggcccttcac cacttcggcg ggaacgccgg ccgcggcgat
     4081 cacctcttga accgcggcac gcatggcgac gaaatcgata tgggtgagca ccagggtgcc
     4141 atccagatcg aacagaaccg cgctgacgcg gccgctgggg cgagctgttg cttccgtgtt
     4201 cacagcccta ttataaggaa cgggggaggg aacgtcaact tagccgctca gattccgagc
     4261 atggcggcgg cttcacgcca ggtgcgctcc agggtgcttt ggggatacca gcctgcgcta
     4321 gaatgacgcg actcgataaa gagcaagaag gaaaggcgct tcaccgggtc gatggcggtc
     4381 cgtgccacga tgcggtccca atccacctgg tcgtgccagc gatccaggat catctccgcg
     4441 tcccaccaat ccgctcggtt ctcatgctgc gccaggatct tctggccgat cagctcctcg
     4501 ggagcggcga cgagaaacgt ctccgtgccg atggcaatcg gccgggcgtg atccagccac
     4561 tcctgagtga ttgggacgat cccacggtgc gtggtgctgc cgaagatcag atcgaccatg
     4621 atgccatcgc actgcgcctg atagagccag cgtggatcgg atcgacggat gaagaagcgt
     4681 gcggtttcca gcgccgtgat cgcacgttcg gcatccgcgg gcaggatcaa gaaatcgata
     4741 tcctttgtcg gccggcggtg cgcatagcag gccacgccgt atccgcctgc gaccatatag
     4801 ggaatcccgg cgtgctcgat ggcgtttcgt gcacagacgt aaacctccat ctccttcgct
     4861 gctacctcga acgccggcat ggcgcagctc ctctcggcga gggccgttgc agtgcccctc
     4921 aggccctggt agagggagtc tgccccatgc cgaacgaggc caaacgtgat cacaaggcaa
     4981 ttggcgcatg ctgtcgaatg gagtggatga gggagaagat ggcggttatt gagcaggtga
     5041 tttgtgttga ggcgccagtc gaaaaggtct tcgactacat tggccggccg gagaacaaca
     5101 aggagtggat cccggatgtt gtggagagcg aaaagctgac ggagggtccg aaccggcctg
     5161 gctctcgctt ccgtttcgtg acgcgcgcgc cgtttggcct gcgcgtttcg gcggaggcgg
     5221 agatcacggc gatggatccc ccccggatgc tggagttcca aagcacctcc ggcgtggagc
     5281 accggggacg ctgggagctg gagagcgagg gtcagataac tcgcgtgcgt ttccgactcg
     5341 agtatcggct cagcggcctg ggttccgtgg tcatgcgggc cgcggggctg aatcagttcc
     5401 tctcacagca tgtgcagggg agcatcgatg gcctgcggcg cgccctggag aatggcaaag
     5461 tccggccgta acccgggtca gtagggagag ggcgcttcgc agatgcagtt gcgcccgctc
     5521 gccttcgcgt cgtacagcgc gcggtctgcg cgttccaccc accgggtggt ggtgtcgccc
     5581 gattgcacct cggcgatccc gatggacgcg gtgatggcga tggtgtgcgc ttcataggcg
     5641 acgcgcaggc cgcggatcga atcgagaatg cgctctgccg agacgacgcc ccgtttgagc
     5701 gacgtgttcc agaggatgac ggcgaactct tcgccaccaa agcgagcgac gaagtcgttc
     5761 ttgacgcggc acgtccgagt caggcaatcg gcgacgctcc gcagcacctc atccccggcc
     5821 acgtggccat aagtgtcgtt cacctgcttg aagtgatcca ggtcgatcat cagcaggcag
     5881 gcggattggc cgaagacgcg atgcatggtg agcgcctgcg ccaagcggtc gtcgaacgcc
     5941 cggcggttgt agaggcccgt gagaggatcg agggcgcttt cctggcgagc cacttccagc
     6001 tgctcgccca gctctctcag gcgagagtga aggccctgca tctgttcacg ctgctgtagc
     6061 ctgcgctcga gagcgatccg cccgatggcg gagaccgcgg agatcacggc gcgccggatg
     6121 c
//
LOCUS       DAOSCV010000128         6118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8185_length_6118_cov_3.105723, whole genome shotgun sequence.
ACCESSION   DAOSCV010000128 DAOSCV010000000
VERSION     DAOSCV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8185_length_6118_cov_3.105723"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>396)
                     /locus_tag="PLY56_04550"
     CDS             complement(<1..>396)
                     /locus_tag="PLY56_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20782.1"
                     /translation="NASHTHPPGPLLCDDAAQVERVFDALRRAQEGMVPARAGAGSGR
                     ESRITMNRTLRLKNGRHWTLRHAYPSPPDQEVAAVGPIDPEIGILRVDHADGRPLAVV
                     YNFACHPLFGDAEGSLTANFPGVASRVIE"
     assembly_gap    397..406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<407..697)
                     /locus_tag="PLY56_04555"
     CDS             complement(<407..697)
                     /locus_tag="PLY56_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20783.1"
                     /translation="MSRADLGQAPAAGLRAGTAWCDLTTDRKGARVRDPLLAKVLVLD
                     DGHTQVGIVAMDTTAIGGRAISRGALPDVGEGFLPALRARVEEELGIPGANLL"
     gene            complement(739..2700)
                     /locus_tag="PLY56_04560"
     CDS             complement(739..2700)
                     /locus_tag="PLY56_04560"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ20784.1"
                     /translation="MRYHDYPKQLEEQYIARMRETYARRVQRLAAIRSPEDARRYIAE
                     VRQAIANAFGPLPERTPLNARVWRVSEQEGFRIEHLTFESRPGFLVSANLYLPSAAAG
                     PAPGVIITCGHSHNGKAYERYAGACVRLAREGYAALIYDPVNQGERELYSLLDTGGRL
                     SRNNNCDGHNIIGRQLQACGDWFGAWRLWDGIRALDYLASRPEVNPDQLAVTGQSGGG
                     TLSAYLWAMEPRFKAVASSCWCTSYLRDLENAMPADEEQYPPGLLAAGFDKIEFFIAR
                     AGEPALLLGQEQDFFDDRGLRAGYEELRRIHEILGGEPDTCGLSMDVQEHAFSEANQV
                     AMVTFFNRVFGKPAPAPQRPLPRLEEEDLKVTPEGDVCRAGSRPMYELVAEQARRVAA
                     AREPVSPEALPERIACALKVRVPDAVPHHRRLFQTRSKSAATGHQVFRFVVESDPGIC
                     CVLRHVCREGSPFRLNPAASAVLYLPNFDSQQELERGGALASLDDFWMLDVRGLGEGL
                     YDLKDLYRLYGHDYMAAGHAVLYGETLLGARVRDVLAAVRLLRAEGAKEVHLIGRKQG
                     AVLAVLAGVLDPEIATVASREAPESFLALATAPFTFWPAVNFPRGVLSAFDLPEARQV
                     LGSRLVESTFSSPVEFADPANDTPEAQGA"
     gene            complement(2751..4664)
                     /locus_tag="PLY56_04565"
     CDS             complement(2751..4664)
                     /locus_tag="PLY56_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20785.1"
                     /translation="MTKLRATMDARVRLARASALQVALVALYTLFTAGASAQDSWDAR
                     RAKLGSKEEPYRILVDKVLMASNDWVMTPEHVAEIKAAGFNVVVPRTGADENERVERV
                     ARMAMEQGLFYMPWIRGTRVETGDLKLRATDELGRTGSLASPNADALWNYWRDRILFY
                     ARLSREVPSVLGVFLDFENYDRVKIGGGMCYTLSYDEPILRQFAQARGLSLPDPLPAN
                     RAEWLEKQGLSKAFEEFQVGWWRERARKLRQEVDALNPRFQFVVYPATHSLFIREVVW
                     REWHTKQAPLIMAEVDTYWRHEYDLADALERLRQIMIKAREALDQVDPAIRYMAGLDP
                     IVSGANPEFEGKSAVLGAEFGHGYWVFYEGPDYHKDHPEYFRWFKRANDHIARRDYSL
                     WRQPAETPNPFDAAVEGKARAVAGAKLVPFNATDPVPQDALERGFIHRTEATYQVLLK
                     QGERLRGELVALQLAHIRHPSVMAVVSPSGKLLTTVRAEVGKPVPIDLVAPEEGVYGI
                     AVSSGRARGQLKLQNRYVSLTGKKLWLVHSQPAAYLAPEADAERFALTISSDAPAEHV
                     QVTFRSPDGAVLVDKNTREEPAMRVRGEVKPARGAWKLDLANAVEDIHIEFGEGCQPR
                     LATHPGRLLVAAP"
     gene            5837..>6118
                     /locus_tag="PLY56_04570"
     CDS             5837..>6118
                     /locus_tag="PLY56_04570"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ20786.1"
                     /translation="MDLDNTPAQGNGHSSTVPGGPGSGPQPAAWQDPSAALHEYVRIL
                     VNRRWSILLCFLGVFLSAAVYVYSQPRIYRATAKVQVVQTVQPILLSEQR"
BASE COUNT         1010 a         2066 c         1987 g         1045 t
ORIGIN      
        1 cctcgatcac ccgggaagcg acgccgggga agttggcagt gaggcttccc tccgcgtcac
       61 caaagagcgg atggcacgcg aagttgtaga ccaccgcgag cggccggcca tcagcatggt
      121 ccacccggag gatgccgatc tcgggatcga tggggcccac tgcggccacc tcttgatcgg
      181 ggggtgaggg gtacgcgtgc cggagcgtcc agtgtctgcc gtttttcagg cgcagggtcc
      241 ggttcatcgt gatccggctc tcgcgcccgg acccggcgcc ggcccgggcg ggcaccatcc
      301 cctcctgcgc ccggcggagc gcgtcaaaga cgcgctccac ctgcgcggcg tcatcgcaga
      361 gcaacggccc aggcgggtgc gtgtgcgagg cgttgannnn nnnnnncagg agattggccc
      421 ccggaatgcc cagctcctcc tccacccggg cacgcaacgc gggcaggaag ccttctccaa
      481 catcgggcag cgccccccga ctgatcgccc gcccgccaat cgcggttgtg tccatcgcga
      541 cgatccccac ctgggtgtgg ccgtcgtcca ggaccagcac tttggcgagc agcgggtcgc
      601 gaacccgcgc gccctttcga tcggtggtca ggtcgcacca cgcggtgcca gcgcgcaggc
      661 cggccgcggg cgcctgcccg agatccgctc tactcatcgc gttcgcctct ctcccatccc
      721 catgcggctc cggtgacatc aggcaccctg ggcttcaggc gtgtcattcg cagggtcggc
      781 aaactccacc ggcgaggaga aggtgctctc caccaggcgc gagcccagca cctggcgcgc
      841 ctccggcagg tcgaacgccg acagcactcc ccgcgggaag ttgacggccg gccagaaggt
      901 gaatggcgcc gtggccagcg cgaggaagct ctccggggcc tcgcggcttg ccactgtcgc
      961 gatctccgga tcgaggacgc cggccaggac cgccagcacc gcgccctgct tccgcccaat
     1021 caagtggacc tcctttgcgc cctcggcgcg gagcaggcgg acggcggcca ggacatcgcg
     1081 cacgcgcgcg cccagcaacg tctcgccgta gaggacggca tgcccggcag ccatgtaatc
     1141 atggccatac agccggtaga ggtctttcaa atcatacagt ccctcgccca ggccccggac
     1201 gtcgagcatc cagaagtcat ccaggctggc cagcgcgccg ccacgctcca gctcctgctg
     1261 gctgtcgaag ttcgggaggt agaggacggc gcttgccgcc ggattgagcc ggaaaggcga
     1321 gccttcgcgg cagacgtggc gcaacacgca gcagatgccg gggtcactct ccaccacgaa
     1381 tcggaacacc tgatggccgg tggcagcgga tttcgatcgc gtctggaaga ggcggcggtg
     1441 gtggggcacc gcgtccggca cgcgcacctt cagggcgcac gcgattcttt cgggcagcgc
     1501 ctcgggcgaa acgggctcgc gggcggcagc gacgcggcgt gcctgttcgg ccacgagctc
     1561 gtacatcgga cggcttccgg cgcggcagac gtcgccctcg ggggtaacct tcagatcctc
     1621 ctcctcgagg cggggcagcg ggcgctgggg cgcgggtgcc ggcttcccga agacgcggtt
     1681 gaagaaggtc accatcgcga cttggttggc ttcgctgaag gcgtgctcct gcacgtccat
     1741 ggagagcccg caggtgtcgg gttcgccgcc caggatctcg tggatccggc gcagctcctc
     1801 gtagccagcg cgcaggccgc ggtcgtcgaa gaagtcctgc tcctggccca acaagagcgc
     1861 gggttcgccc gcgcgggcga tgaagaactc gatcttgtcg aagccggcgg cgaggaggcc
     1921 gggaggatac tgctcctcgt cggccggcat ggcgttctcg aggtctctca gatagctggt
     1981 gcaccagcag gaggatgcga cggccttgaa ccgcggctcc atcgcccaaa ggtaggcgct
     2041 cagcgtgccc ccacccgatt gcccggtgac cgcgagttgg tcggggttca cctccgggcg
     2101 cgatgccaga tagtccaggg cgcggatccc atcccacagc cgccaggcgc cgaaccagtc
     2161 gccacacgcc tgaagctgcc gtccgataat gttgtgcccg tcgcagttgt tgttgcgcga
     2221 gagccgcccg cccgtatcga gcaggctata cagctcgcgc tcgccctggt tcaccgggtc
     2281 gtaaatcagg gcggcatacc cctcgcgggc caggcggacg cacgccccgg cataccgctc
     2341 gtatgccttg ccgttgtggc tatgcccgca cgtaataatc actccaggcg cgggtcccgc
     2401 cgccgcggat ggcaggtaga ggttggccga gaccaggaag ccgggccggc tctcgaaggt
     2461 gaggtgctcg attcggaagc cttcctgctc ggacacccgc cacacgcgcg cgtttagcgg
     2521 agtgcgctct gggagcgggc cgaaggcgtt ggcaatcgcc tgccgtacct ccgcgatgta
     2581 gcggcgggca tcttctggcg agcggatcgc cgcgaggcgc tgcacgcgcc gggcatacgt
     2641 ctcgcgcatc cgcgcgatgt actgctcctc gagctgcttc gggtagtcgt gatagcgcac
     2701 tggtttcctc ccaaattgca cacgggcggt ttctggcgtg cccggcctgg ctacggggcc
     2761 gcgacgagca ggcgcccagg gtgcgtcgcc aggcgaggct ggcagccctc gccaaactcg
     2821 atgtggatat cctcaaccgc gttcgccagg tccagcttcc aggcgccgcg cgccggcttc
     2881 acctcgccgc gcacccgcat cgcgggctcc tcgcgcgtgt tcttatcgac gagcacggca
     2941 ccatcgggag accggaacgt cacctggacg tgttcggcgg gcgcatcgct actgatggtc
     3001 aacgcgaagc gctcggcgtc cgcctcgggg gcgaggtagg cggccggctg gctatgcaca
     3061 agccacagtt ttttgccggt cagcgaaaca tagcggtttt gcagcttaag ctgccctcgc
     3121 gcacgcccgc tgctcacggc gatcccgtaa acgccctcct ccggggcgac gagatcgatg
     3181 ggaaccggct tgccaacctc cgcgcgcacc gtagtgagca gcttccctga aggggagacg
     3241 acggccatca cgctggggtg gcggatgtgc gccagttgga gcgcaaccag ctcgccgcgc
     3301 aggcgctcgc cctgcttcag gagcacctgg taagtcgcct cggtgcggtg gatgaagccg
     3361 cgctcgagcg catcctgggg aaccggatcc gtcgcgttga acggaacgag cttcgcgcct
     3421 gcgacggccc gagccttacc ctcgaccgcc gcgtcgaacg ggttcggggt ttcagccggc
     3481 tgccgccaga gcgagtagtc acgccgggca atgtggtcgt tcgcgcgctt gaaccagcgg
     3541 aagtattcgg gatggtcctt gtggtagtcc gggccctcat agaagaccca atagccgtgg
     3601 ccgaactccg cgccgagcac agcactcttg ccctcaaact cggggttggc accactcacg
     3661 atcgggtcga gaccggccat atagcggatc gccgggtcga cctggtcgag cgcttcgcgg
     3721 gccttgatca tgatctggcg caggcgttcc agggcatccg ccaggtcata ctcgtgccgc
     3781 cagtaggtat cgacctcggc catgatcagt ggggcctgct tagtgtgcca ctcgcgccag
     3841 acaacttccc ggatgaagag ggagtgggtg gctggataga cgacgaactg gaagcgcggg
     3901 ttgagggcat ccacctcttg gcgcagcttg cgggcgcgct cgcgccacca gcccacctgg
     3961 aactcctcga acgccttgga gagcccctgc ttttcgagcc actccgcgcg gttggcggga
     4021 agcgggtccg gcaggctcag cccgcgggcc tgagcaaact ggcgcaggat cggctcgtca
     4081 tacgagagcg tgtagcacat cccgccgccg atcttcaccc ggtcgtagtt ctcaaaatcg
     4141 agaaagacgc ccaggaccga gggcacctcg cgagagagcc gggcgtagaa gagaatgcgg
     4201 tcgcgccagt agttccagag cgcgtcagcg ttggggctgg caagcgaccc ggtgcgcccg
     4261 agctcatccg tggcccgcag cttcaggtcg ccggtctcga cgcgcgtgcc acgaatccag
     4321 ggcatataga agaggccctg ctccatcgcc atacgcgcca cgcgctccac gcgttcgttc
     4381 tcatccgcgc cggtgcgcgg cacgaccacg ttgaacccgg ccgccttgat ctcggccacg
     4441 tgctccggcg tcatcaccca gtcattgctc gccatgagca ccttgtcgac caggatccgg
     4501 tagggttctt ccttgctgcc cagcttcgcc cggcgggcat cccacgagtc ctgcgcgctt
     4561 gcaccggctg taaagagcgt gtatagagcg accagagcca cctgcaaggc gctcgcgcgc
     4621 gccaggcgca cccgggcatc catcgttgct cttagtttgg tcattaccat agtcccttgc
     4681 gtcctttcgg caacggggca ggctggatga gggccggccc tctcccgctt agcgctcaga
     4741 aggtgagttc aaccttcagg ggccaatccc cttgcgcacg cagagagcaa gttggcccac
     4801 actgggcccc gctttcatgc ttaaacgctt gacacaacca cacctgagtg ctatactttg
     4861 ggtcgtgagg cacgcccctt gatggacaac aggggcgcca ctatcgatag ctggacggcg
     4921 gcctattggc ctgagcacgg agcttcaccc gccgtcgggg caaagtcaaa gtttcccttg
     4981 ccaccgaccc ctatccttgg ccagccatac gtccagtgta gaactggggc atcctctctt
     5041 cggcgttcga gcatatcatc tctcggttat ccacgccacg tgtgagagtt gctatccaga
     5101 gtcacaggtg cattcatgcc tggagctatt cccgcctcgt gcgaagcacg gtggtgagga
     5161 aaagggcagc gcctgcttcg aagaaggacc ttctctcggt atcagtttca tcgctctctt
     5221 ccagaataag ggctgcttct gcgtggtgat cgccgtgatc gatttcgaac cggcgagcag
     5281 attcgagcac gagtgcgccg tgaccgcagt gccgggcaaa tagacccggc gaagggtggt
     5341 gcgcatgact ccctccggtg aaacccagtg tgcaacgcct gcaccgaccg ttgcctgctg
     5401 tccttatctc ggtcatcgtg acccaggcga gaagccacgc ccctacgcaa cgcgacacaa
     5461 cgtctgctat gcgcggcgag gtcgctccca cgcctattcg agcgtgacgc gtgaggtgca
     5521 gatggagagc tgcttggagc cagcaggggg ctggccaatg tgtcgccact acgcggcggc
     5581 aatggccgag caccgggcgg ccccgcacgg agcacacgga atgccggcgg gggttcactc
     5641 ggccctggtg cgccgtcgtt cgcgccgagc gtcccggtca cgctctctgc ggcgccgctt
     5701 gatgcgccag ctcgccccgc tcttctggtc tttctgtatc atcgccgtct cagtactcct
     5761 cggcgccttg atcgcgagga gttgcggctc tgcccggtaa cctcgggcac accacccgaa
     5821 cattgaggat tccattttgg atctagataa cactccagct caagggaatg gccattcctc
     5881 caccgttcca ggaggtccag gaagcgggcc gcagccagcc gcgtggcagg atcccagcgc
     5941 cgctctgcac gagtacgtac ggatcctggt caatcggcgg tggagcattc tgctttgctt
     6001 cctgggagtc ttcctctcgg cggcagttta cgtttactcg cagccgcgga tctaccgcgc
     6061 caccgccaag gtgcaggtgg tccagaccgt gcagcctatc ctgctgagcg aacaacgc
//
LOCUS       DAOSCV010000129         6111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8195_length_6111_cov_3.904227, whole genome shotgun sequence.
ACCESSION   DAOSCV010000129 DAOSCV010000000
VERSION     DAOSCV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8195_length_6111_cov_3.904227"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLY56_04575"
     CDS             <1..1393
                     /locus_tag="PLY56_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ20787.1"
                     /translation="KGTHVDSIFYCTWSSGFGYFTHRTKVGQIFDRTDNPEHPDNKGG
                     GLSRNKTAEFIRQGTDPLEIMVDFCKENGIEVFWSLRMNDTHDAWGSWYGDLLFPQVK
                     KDHPEWLVASKEKRSKHGGWSAMDFGHAEVRDLTFRFFEEVCENYDVDGIEMDFCRHP
                     VYFKNPAWGIDAGPTEWEQMTEMVRRVREMTERVGLKRGRPILVAARVPDSVEYCKAM
                     GLDLERWLQEGLIDLLAVSDYFRLNPWKVSVDLGRKYGVPVYPCLSETRMRDDEARKT
                     RSSIECYRSRAMNAWAAGASGIYLFNSFNPRSPLWSELGDPKVLETLDKVYVIGARGV
                     AVLNFWMADGEKRFMKRPTLSPERPVALKPGEAFRVPLEVGEEVREKAGATPRVTLQV
                     RVADLPEGALLQVTLNDKPLSGAVREGDWFTYTVAPAAVRKGTNEIETVLQPDTPDGA
                     KLLDVLLWVRYNR"
     gene            complement(1381..2481)
                     /locus_tag="PLY56_04580"
     CDS             complement(1381..2481)
                     /locus_tag="PLY56_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOJ20788.1"
                     /translation="MHRERESGQSLVLLAVALSTLLGFMALTVDVGRWFVARQRAVNV
                     CDMAALAGAQDLSLTVDAASARRVQSVVLSIVNANNAEGFAISMSQVLGPDNPDIIIS
                     EFNDTVATNSALDSNIVWTDSQGNVVPRPGPGRGILVRGQIPVQPLFARIFGITGAKV
                     IPAQTAVALGTVSTITGNLVPFGVSVHGIVDIESSPSATIEQQLTYRSWKDSWTGTPG
                     AFGTLGLTHTSGTTLREEIQWGHFGTYGVGQYVAMTSGNKTGPIQQGTDGRIARSQYA
                     TIDEWLAAFHSDDPVAHQEALQDSRVVLIPIVDETQIIGESVRIVGFATYAIAEWRNG
                     EKKVYGYFVKSTYQGSVGEGLAGFGTRSYYLL"
     gene            2890..4170
                     /locus_tag="PLY56_04585"
     CDS             2890..4170
                     /locus_tag="PLY56_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20789.1"
                     /translation="MSSLRVSATAVPIPHAWEAAGRPLEAPLRVSALALSAGKPFCFL
                     TSDTLEVPADIYEEVARRLETEAGVSPDRILLCATHSHHAPSTIDHLGMRRDRDFCAS
                     LQETLVRAALDALRGLDSSQSGCRLLFAESQEATVGMNSRYLLKDGTIAWYAYAWEDV
                     IRPTGPYDPDLPVLAAVAPSGETRAVLFSHSVHNIGTLSRETYSPGTYGLAAQELERQ
                     HQGVTLFLPGAFGSTHYTGGFGATPLPYALTTAERVYRIVEAVKEGLDRAVAMDVDAI
                     QALRRPFTYRLRTFDEAKEAATVQRWAEKYTPREAASHCRAFAEMRREMAPVQGEERQ
                     TWLHAIRLGDIALVGIPGELFAHLGLAIRRRSPFRHTYVIGLANGTIGYIGDRKAYEL
                     GGYQLWAGWHSRSEPGTGEAMVDQAVAMLEELAA"
     gene            4260..5639
                     /locus_tag="PLY56_04590"
     CDS             4260..5639
                     /locus_tag="PLY56_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20790.1"
                     /translation="MIHWSAEAHRRRLQNIGLAERSVRRALRRHLIRDYLPGQVTYNL
                     GEYPCARPYEPSEADERLLDEYRDAGVELIQVHEEWNDAQRLFGGDKFSPVNPSGLRR
                     FLEMAHSRGLKVLLYASTGFFEMRDPDFRPEWARPGNVLAEAWYQYARCSPASHSWRA
                     YLLPRLLGLMDEWGADGIYNDCGYNRAAWNLPPTPDEVLAFEEGPDHDAAIADLLALV
                     YAEVKRRGGIFKLHFSGTGAPRTGLRTYDYLWVGEGVQDLDAQREVVKHHEPYVIPGF
                     DLSRAQPAGEDELYLHTIPYLQFPLLIGGQPVTGERMMQPGLEYKDEATDFWTRHMRA
                     IYRYAQEHPEGPHSYGWWDSVPGRPDAKERFFHWLRLYGPMVTEGTWAYLEITESDLF
                     RAKLPEGVVASLFANEELWLVLANYGRSAVEVPTRDTYTPVSLPGQPGTLFQVPARSL
                     LILRRHEAG"
     gene            complement(5739..5945)
                     /locus_tag="PLY56_04595"
     CDS             complement(5739..5945)
                     /locus_tag="PLY56_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20791.1"
                     /translation="MRVTMARGTRAFRLPAEPKSRFLEDEEGELWVVQQVTRVNGEYE
                     VLCRHATRIEQRLYEREQQAASGA"
BASE COUNT         1047 a         1982 c         2015 g         1067 t
ORIGIN      
        1 caagggaacc cacgtcgatt cgatcttcta ctgcacctgg agctccggct tcggctattt
       61 cacccacagg acgaaggtcg ggcagatctt cgatcgcacc gataacccgg agcatccgga
      121 taacaagggc ggcgggctat ctcgcaacaa gacggccgag ttcatccgcc agggcaccga
      181 tcccctcgag atcatggttg atttctgcaa ggagaacggg attgaggtct tctggtcgtt
      241 gcgcatgaat gatacccacg acgcttgggg gagttggtat ggcgatctcc tcttccccca
      301 ggtgaagaag gaccatcccg agtggctcgt ggccagcaag gagaagcgct cgaagcatgg
      361 cgggtggtcg gccatggatt tcgggcacgc ggaggtgcgc gacctgacct tccggttctt
      421 cgaggaggtc tgcgagaact acgacgtgga cgggatcgag atggattttt gccggcaccc
      481 ggtctacttc aagaacccgg cctggggcat cgatgccggc ccgaccgagt gggagcagat
      541 gactgagatg gtccggcgcg tccgagagat gacggagcgc gtgggcctga agcgcggccg
      601 gccgattctc gtggcggcgc gcgtgcccga ctcggtagag tattgcaagg cgatggggct
      661 ggatctggag cgctggctac aagagggcct gatcgacctt ctcgccgtga gcgattactt
      721 ccgcctcaac ccctggaagg tgagcgtgga tctggggcgc aagtatggcg tgccggtcta
      781 cccctgcctg agcgagacgc gcatgcgcga cgacgaagcg agaaagacgc gctcttccat
      841 cgaatgctac cgctcacgcg caatgaacgc ctgggcagcc ggcgcatcgg gcatctacct
      901 tttcaactcc ttcaacccgc gcagccctct ctggagcgag ctgggcgatc cgaaggtgct
      961 ggagacgctc gataaggtgt acgtgatcgg cgcgcgtggc gtcgccgtcc tcaacttctg
     1021 gatggcggac ggggagaagc ggttcatgaa gcggcccacg ctctcgccgg agcgccccgt
     1081 cgccctaaag ccgggcgagg cgttccgcgt gccactggaa gtcggcgagg aggtacgcga
     1141 gaaggccggt gccacgcccc gggtcactct ccaggtgcgc gttgccgacc ttcccgaagg
     1201 agcactgctc caggtcacac tgaacgacaa gccgctctcc ggcgccgtgc gggagggcga
     1261 ctggttcacc tacacggtcg ctcccgccgc ggtgcgcaag gggacgaacg agatcgagac
     1321 ggtgctccag ccggacacgc ccgacggcgc gaagctgctc gatgtcctgc tttgggtgcg
     1381 ctacaacagg tagtaggagc gggtgccgaa ccccgccaga ccctcgccga cggagccctg
     1441 atacgtcgac ttgacgaagt agccgtacac cttcttctcg ccgttgcgcc attcggcgat
     1501 ggcataggtg gcgaagccga caatccgaac cgattcgcca atgatctgcg tttcatcgac
     1561 gatcgggatc agcacgacgc gcgagtcttg cagggcttcc tggtgcgcca ccgggtcgtc
     1621 cgagtggaac gcggcgagcc actcgtcgat agtggcgtat tgcgaccgcg cgatgcgccc
     1681 atccgtgccc tgctggatcg ggccggtctt gttgcccgag gtcatggcga cgtactggcc
     1741 cacgccgtag gtgccaaagt gaccccactg gatctcttcg cgcagggtgg tgccgctggt
     1801 gtgcgttagc cccagcgtgc cgaaggcgcc aggcgtgccg gtccacgaat ctttccacga
     1861 gcggtaggtg agctgctgct cgatggtggc gctcgggctg gattcgatgt cgacgatccc
     1921 atgcacggaa accccgaaag gtaccaggtt gccggtgatc gtcgagaccg tgcccagggc
     1981 gacggcggtc tgagcaggga tcaccttggc gccggtgatt ccgaagatcc gggcgaagag
     2041 cggttgcacg gggatctgcc cgcgcaccag aatgccccgg cccgggccag ggcgaggcac
     2101 cacgtttcct tggctgtcgg tccagacgat gttgctgtcg agcgcggagt tggtcgcgac
     2161 ggtgtcgttg aactcggaga tgatgatgtc ggggttgtct ggtccgagca cctggctcat
     2221 cgagatggcg aagccctcgg cgttgttggc attgacgatg gagagcacga ccgattgaac
     2281 acgccgggcg ctcgcggcat ccaccgtcag cgagaggtcc tgggcgccgg cgagcgcggc
     2341 catatcacag acgttcacgg ccctctgacg tgccacgaac cagcggccta catccacggt
     2401 gagcgccatg aaaccgagaa gagtcgatag cgcgacggct agcagaacca gagactgccc
     2461 gctttcacgt tcccgatgca cggtctcctc cctcccacaa ggccgatgcc tgcaacaaac
     2521 cacgagcagg ccggccatcc cccataccac gacacgtgtg gtacatggcg gcggttcatt
     2581 cactctccat tatggcgttt gatcgggaga gcttgaggga cgagcgcttc cgcagggctg
     2641 ctacccacgc cggcgtccag ctcccgtggt gatctcgccc ggcaggaacc gagaaggccg
     2701 cgcggaaaca agggggtgtt ggcgggtcgc cacgcccctc gatggaggag cgcgcggttg
     2761 ccacgcaaat agggtggtgc gatggggatc caccgtggcg cgctccacgg tgccggtcga
     2821 atgccggcgc ctgtcgagga aggtgttggc gcttcgcccc gcgcacgcac ggccaggagg
     2881 tgcacgagga tgtcttcttt gagggtttct gcgacggcgg taccgattcc gcatgcctgg
     2941 gaggcagccg ggcgcccgct ggaggctccc ctccgcgttt cggctctcgc gctctccgcg
     3001 ggcaagccct tctgcttcct caccagcgac acgctggagg tgcccgccga tatctacgag
     3061 gaggtcgcgc gccggctcga gacggaggcg ggagtatcgc cggaccgcat cctcctctgc
     3121 gcgacgcact cccatcacgc gcccagtacc attgaccatc tcggcatgcg gcgcgaccgc
     3181 gacttctgcg cctcgctcca ggagactctg gtccgtgccg ccctcgatgc cctgcgcggc
     3241 ctcgattcat cacagagcgg ctgccgcctt ctcttcgcag agagccagga ggcgaccgtc
     3301 ggcatgaaca gccgctatct cctgaaggac ggcacgatcg cctggtacgc gtacgcctgg
     3361 gaagacgtga tccgccccac cggaccgtac gacccggatc tccctgtgct cgccgcggtt
     3421 gccccctcgg gcgagacacg cgccgtgctg ttcagccatt cggtgcataa catcggcacg
     3481 ctatcgcgcg aaacctactc cccgggaacc tacggcctgg ccgctcagga gctggagcgc
     3541 cagcaccaag gcgtgacgct tttcctgccg ggggctttcg gctcgacgca ctatacgggc
     3601 gggtttggcg ctacgccgct tccctatgcc ctgaccaccg ccgagcgggt ttaccgcatt
     3661 gtggaagccg tgaaagaggg cctggaccgc gcggtggcca tggacgtgga tgccattcag
     3721 gcgctgcgcc ggcccttcac ctatcgcctc cgcacctttg atgaagccaa agaggccgcc
     3781 acggtgcagc gctgggccga gaagtacact ccgcgtgagg cagcgagcca ttgccgagcc
     3841 tttgccgaga tgcgtcggga gatggcaccc gtgcaaggcg aggagcgcca gacctggctc
     3901 cacgccatcc gcctgggaga tatcgctctt gttgggatcc cgggagagct gtttgcccat
     3961 ctggggctgg cgatccggcg tcggtctccc ttccggcata cctacgtcat cgggttggcc
     4021 aacggcacca tcggctacat cggcgaccgc aaagcctacg aactgggtgg ctaccagctc
     4081 tgggcgggct ggcacagccg ctccgagccg ggcaccggag aggcgatggt tgaccaggcg
     4141 gtcgcgatgc tcgaagagct cgccgcgtag ccgcgcgagg ccactcgtcg gccctggctt
     4201 cgcggcactt ctcaccacca gggcctgcta tcagatcgag tatctggagg aacaccggaa
     4261 tgatccactg gagcgcggag gcgcaccgcc gtcgcctgca gaacatcggc ctcgcggaga
     4321 ggagcgtgcg ccgggcactt cgtcgccatc tcattcgcga ctatctgccc gggcaggtca
     4381 cgtacaacct gggggagtac ccctgcgcgc gcccctacga gccgtccgag gcggatgagc
     4441 gcctgctgga cgagtaccgc gacgccggcg tggagctgat ccaggttcac gaggagtgga
     4501 acgatgccca gcgtctcttt ggcggtgaca agttctcccc ggtgaaccca tctggcctcc
     4561 gccgcttcct cgagatggcg cactcgcgcg ggctgaaggt cctcctctac gcttccaccg
     4621 gcttcttcga gatgcgtgat cccgattttc gcccggagtg ggcccgcccc ggaaacgtgt
     4681 tagcggaagc gtggtaccag tatgcgcgct gctcgcctgc cagtcactcc tggcgggctt
     4741 acctcctgcc gcgcctgctt ggcctgatgg atgagtgggg cgccgacggg atctataacg
     4801 attgcggcta caaccgcgcg gcctggaacc tgcctcccac tccggacgag gtgcttgcct
     4861 tcgaggaggg ccccgatcac gatgcggcga tcgctgacct cctcgcgctc gtctatgccg
     4921 aggtgaagcg gcgtggcgga atcttcaagc tccatttcag cggtaccggc gcgccacgca
     4981 cggggcttcg tacctatgac tatctttggg tgggcgaggg ggttcaggat ctggatgccc
     5041 aacgggaagt ggtgaagcac catgagccct acgtcatccc cggcttcgat ctctcgcgcg
     5101 cccagccggc aggggaagac gaactctacc tgcacacgat cccctatctg caattcccgc
     5161 ttctcattgg cggccaaccc gtcaccggcg agcggatgat gcagcccggg ctggaataca
     5221 aggatgaggc cacagacttc tggacacggc acatgcgcgc catctaccgc tatgcccagg
     5281 agcatcccga gggcccgcac tcgtacggct ggtgggactc cgtgccaggc cgaccggatg
     5341 cgaaggaacg cttcttccac tggctgaggc tgtacgggcc gatggttacc gagggcacgt
     5401 gggcctacct ggagatcact gagagcgacc tgttccgagc gaagctgcca gaaggggtgg
     5461 tcgcgtcgct ctttgccaat gaggagctat ggctcgtcct cgccaactac gggcgctccg
     5521 cggtcgaggt acccacgcgc gacacctaca cgccggtgag tctgccgggc caacccggca
     5581 cgctcttcca ggtgccggcg cgctcgctgc tcatccttcg ccgacacgaa gcagggtagg
     5641 ggtgcccggc ggccgctcgt gcgggcgcgc ccgcatcaac gcgcgattcc ggtaacggcg
     5701 tggcatccgg acaaccgtga atgtagtccc ggggccggct aggcgccaga cgcggcctgc
     5761 tgctcccgct catagaggcg ctgctcaatg cgagtggcgt ggcggcagag cacttcgtac
     5821 tcgccgttca ccctggtcac ctgctgcacc acccacagct ccccttcctc gtcttccagg
     5881 aatcgagact tcggctccgc tgggagccgg aacgctcgcg tgccccgtgc catcgttact
     5941 cgcatgcgag acctccccat cgcgcgttct tgtgtggtaa gactcctacc ccgttgcggc
     6001 gggatgcaac acggagccgg ggggcaggag caggtttacc gcaggcttgg gtggtgcata
     6061 atacaggaaa gatcggatcg aacggcaaca gcttctggag gtggggcgat g
//
LOCUS       DAOSCV010000130         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8198_length_6110_cov_3.182989, whole genome shotgun sequence.
ACCESSION   DAOSCV010000130 DAOSCV010000000
VERSION     DAOSCV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8198_length_6110_cov_3.182989"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="PLY56_04600"
     CDS             complement(<1..1636)
                     /locus_tag="PLY56_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20792.1"
                     /translation="MTPLQRALFTVALTGVIGLAGLSVLQAKEASLTVTAQQRANAVR
                     NAARYGWARSERAAAEKAAERWKAMSDEELWALIPSQELPRSIHVYNIAGTNKTALCP
                     ECKEGIIPFGNYPWRCDVFGKPWKVTCPNPKCQAVFPSNDFGAYYRSALDKHGFFRRG
                     KGDPKLLFNTAHPDPNDPLHRAYVDDGYGWTDAQGTRWDFIAVYVQWGLWTEIKRAIA
                     NLTRAYTLTEDPVYAHKCGVLLARLADVYPEMDFWPLHEQGFSHSHGGRGMGKVEGSI
                     WETGNGSSWALAYDQIFDALIRDTELAAFVNEMHRRQELPPTPDPKAVAAHIERGLIR
                     EIITAVKDGRIHGNQGMHQRTMIAAALALDDPKETPQLIDWVFAPGTYQRDPNNPGRE
                     ICTGGSLAQVLVSVMDRDGLGNEGAPGYSLWGASLQPAADLLEDNAKYRARSIYRDFP
                     KYRQYYLACKRWTCLEAATPPIGDSGAASAWGIVAPGAQALLRAYEIYRDPALARFAW
                     QMLGRKLDRVHGSIFSENPEALREEVARLVSGPEPPL"
     gene            complement(1796..5731)
                     /locus_tag="PLY56_04605"
     CDS             complement(1796..5731)
                     /locus_tag="PLY56_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20793.1"
                     /translation="MHLYRLALVLTALSFVLSPACSAPAADADGDGIPDAVETKLGLL
                     PQVKQELVPIATSKNENYSEEQAAQHAPDILALDACHVGGNRLLFRVTFARKPNFIGA
                     TLILYADMDNNPQTGRKDPHHGGVDLMAVVSGRDVSLSLHNNAYDSGNSMAGGTVDGN
                     VLYLVLDAPLAVVGDQIAVGVHLLVQRAGGRSDGTPHRVAMLPRSAQAVPKLDRRQLA
                     DLRGFADYRYHNDVVKLERLEDKGLTYGQVAPKTPITIGRPRPAVPFSATARQPGKSG
                     SATRERVRVHLLEEAGVARKATPIRFGFPLAKGALFDAAQVRILSPGGTEIPCQVTPT
                     SFWPDDSLKWVLVQFAAPLKARESTEYTVEFGSQVKRAAASSPLKATETDAQLTVTTG
                     PLQAVIDKRQFNLLQDVRLDAKGDGRFAETERVAAFGPDGIRLVDERGKLFTASARPP
                     ESVRIEEQGPCMLVVRVEGAYAAADGTTYMRYIARLTFRAGSPRVELALTHLNDYLRT
                     EFTDITSLSLPFAPSGSIRQGTLFLRDKAGKLQPREGRTLSLFQEDEAQCLVRADGQQ
                     SKGGQAPGVIRVSTERGALTVAVRDFWQRWPKGLSCEGNGLSIDLLPAQPGPEYGKNL
                     PHYLLYPFVEGKYRFKWGVSFTERISFDFSGQTTPEEIDAETNAPVIAVLPASWYAST
                     GALGRLAAPLQKQFSAWDAWVAAAYQANERSRVRTREYGYLNYGDWFGERGRNWGNNE
                     YDFAHGFFMQFARTGNRDYFRAALIAARHQADVDCVHAYPDPYYVGANHQHSIGHTGT
                     WSQNPPLATWSHRYDYHTEAANGHTWADGMVDAWYLTGDARVMEAAIGLGEHIVWAAA
                     PAFKALGTHERSAGWSIKAVLAIYRATGDPKYLEAARHIGSVALSEQKFDEGGAWPHT
                     LPRDHAGDRPGARGNNLFLIGILLGGLQALHEETGDPAVRKSLIAAAEWVLKSWDEVA
                     EGWPYSALVTGEPLYKPSTSLNLLIVGPIAYVGHLTGDERFIRVAESGLRGVVRQGAD
                     AFGKSVAQKMHFTSGTLALLQEWYARRPDKGARVLDGSDTGLEEWAARTPVATLHNVR
                     APDEKRFFVRLDGPTGELVAKRSPHGSMMKRAETGTLRVLDAAGKVVHEEQFSTDTAC
                     EIRCPLRGAAGALFRVVIQDDQRSVWDLSGDGLGIVTQTVPGFRFGGVGRARYHFFVP
                     AGTKEFRVHLLGVHSGTYGGAAITPDGKIAAVHQDSNPGQAFIEGAPKGTVDPRHPER
                     GTIVVKPAPADTGKVWSLVVWAAMDLGCELEGVPPYLSRTAQEWFDPGR"
BASE COUNT          954 a         2030 c         2075 g         1051 t
ORIGIN      
        1 ccaggggcgg ctccgggccg gagaccaggc gtgcgacctc ctcgcgtagc gcttccggat
       61 tctcggagaa gatggagccg tgaacccggt cgagctttcg cccgagcatc tgccaggcaa
      121 agcgggcgag cgccggatcg cgataaatct cgtaggcgcg gaggagagcc tgtgcgccag
      181 gcgcgacgat cccccaggcg ctcgcagcgc ccgagtcgcc gatgggaggc gttgcggctt
      241 cgaggcaggt ccaccgcttg cacgccaggt aatactgccg gtacttggga aagtcgcggt
      301 agatgctgcg cgcgcggtac ttggcgttat cctccagcaa gtcagcggca ggctggagtg
      361 aggcacccca gagtgagtag ccgggagcgc cttcgttgcc cagaccgtcg cgatccatga
      421 cgctgacaag cacttgggcc agactgcccc ccgtgcagat ctcccgtccg ggattgttgg
      481 gatcgcgctg gtaggtgccg ggcgcgaaga cccaatcgat cagctggggc gtctctttcg
      541 ggtcatcgag cgcgagcgcg gcggcgatca tcgtccgctg gtgcatcccc tggttgccgt
      601 ggatccgtcc gtccttcacc gctgtgatga tctcgcggat cagccctcgc tcgatgtgcg
      661 cggcaaccgc cttcggatcg ggcgtgggtg gcagctcctg gcgccggtgc atctcattga
      721 cgaaggcggc cagctccgta tcgcggatca gggcgtcgaa gatctggtcg taggcgagcg
      781 cccagctgct tccgttgcca gtctcccaaa tggagccctc cacttttccc atgccccggc
      841 cgccgtgcga gtgcgagaag ccctgttcgt gcagcggcca gaaatccatc tccggataga
      901 catcggccag gcgggccagg agcacgccgc acttgtgggc atacaccggg tcttcggtga
      961 gcgtgtaggc gcgcgtcaga ttggcgatcg cgcgcttgat ctccgtccag agaccccatt
     1021 gcacgtacac ggcgatgaaa tcccagcgcg tgccctgcgc atcggtccag ccatagccgt
     1081 cgtccacgta ggcccggtgg aggggatcgt tcgggtcggg gtgggcggtg ttgaagagga
     1141 gcttcgggtc gcccttgccc cggcggaaga agccgtgctt gtccagggcc gagcggtagt
     1201 aggcaccgaa gtcgttgctg gggaagacgg cctggcactt ggggttcgga caggtgacct
     1261 tccagggctt gccaaagacg tcgcatctcc agggatagtt gccgaagggg atgatcccct
     1321 ccttgcactc cggacagagc gccgtcttgt tggtgcccgc gatgttgtag acgtggatgc
     1381 tgcgcggtag ctcctggctg gggatcagcg cccacagctc ctcatcggac atcgccttcc
     1441 agcgctccgc cgccttctcc gcggctgcgc gctcggatct cgcccagcca tagcgggcgg
     1501 cgttgcgcac cgcgttggct cgctgttgtg ctgtcaccgt gaggctggcc tccttagcct
     1561 ggagcacaga gagccccgcg aggccgatca ctcctgtcag cgccactgtg aacagcgcgc
     1621 gttgcagagg cgtcatcttc ttctgccctt tcgcatgcgg ccggcggcgc ctcgatgggg
     1681 tgacgggctc ccctttgcgc cggtggcaga aagggctttg cccgccggcc aatggggcgg
     1741 gagggagcag ggtcacgccc gcctcccgcc ctacgcctgg aggcctctcc actcgtcagc
     1801 gcccgggatc gaaccactcc tgcgctgtgc gcgagaggta gggaggaacg ccctctagct
     1861 cgcaacccag gtccatcgcg gcccacacga ccaggctcca cacctttccc gtgtcggccg
     1921 gcgccggctt cacgacgatc gtgccgcgtt cggggtgcct gggatccacc gttcccttcg
     1981 gcgcgccctc gatgaacgct tgtccggggt tggagtcctg gtggaccgcc gcgatcttgc
     2041 catccggcgt gatcgccgcg ccgccatagg tgccggagtg gaccccgagc aggtggacgc
     2101 ggaactcctt tgttcctgcc ggcacgaaga agtgataccg cgcgcggcct acgccgccga
     2161 agcggaagcc gggcacggtc tgcgtcacga tccccaggcc gtcgccactc aggtcccaca
     2221 cgctgcgctg gtcatcctga atcaccaccc ggaagagcgc gccagcggct ccgcggaggg
     2281 gacatcggat ctcgcaggca gtgtcggtgc tgaactgctc ctcgtgcacg accttccccg
     2341 ccgcgtccag gacgcggagc gtaccggttt cggcgcgctt catcatggag ccgtggggcg
     2401 aacgctttgc caccaattcg ccggtgggcc cgtccaggcg cacaaagaag cgcttctcgt
     2461 ccggggcgcg cacgttgtgc agcgtcgcca cgggcgtgcg cgcggcccac tcctcaagcc
     2521 cggtatcgct gccatccagc acgcgcgctc ccttatccgg gcggcgcgcg taccactcct
     2581 gcaggagggc cagcgttccg gacgtgaagt gcatcttctg cgcaaccgac tttccgaagg
     2641 cgtcggcgcc ctggcggacc acgccacgca ggccgctctc cgcgacgcgg atgaagcgtt
     2701 catcgcccgt caggtgcccg acgtaagcga tcggtccgac gatcagcagg ttgaggctgg
     2761 tgcttggctt gtagaggggc tcgcctgtca ccaaagcgga gtagggccac ccctcggcca
     2821 cctcgtccca cgacttgagc acccactccg ccgcagcgat gagcgatttg cgcaccgcgg
     2881 gatcgcccgt ctcctcgtgg agggcttgca ggccgccgag gaggatgccg atcagaaaga
     2941 ggttgttgcc gcgcgcgcca gggcggtccc cagcgtggtc gcgcgggagg gtgtggggcc
     3001 acgcgccgcc ctcgtcgaac ttctgctcgc tcagcgccac ggatccgatg tgccgggctg
     3061 cctccaggta cttcggatcg cccgtagccc ggtagatcgc caggacggcc ttgatcgacc
     3121 agccggccga gcgctcgtgc gtgcccagcg ccttgaacgc cggcgccgcg gcccagacga
     3181 tgtgctcgcc cagcccgatc gcggcctcca tcacgcgcgc gtcgcccgtc aggtaccagg
     3241 catccaccat tccatcggcc caggtgtgcc cgttggccgc ctcggtgtga tagtcatacc
     3301 ggtggctcca ggtggccagc gggggattct gcgaccacgt gccggtgtgg ccgatggagt
     3361 gctggtgatt ggcgcccacg tagtagggat ccggataggc gtggacgcaa tcgacatcgg
     3421 cctgatgccg cgccgcgatc agcgccgcac ggaagtagtc gcggttcccg gtgcgcgcga
     3481 actgcatgaa gaagccgtgt gcgaagtcgt actcattgtt gccccagttg cgcccgcgct
     3541 cgccgaacca gtcgccgtag ttcaggtagc catactcgcg cgttcgtacc cggctgcgct
     3601 cgttggcctg ataggcggcg gccacccatg catcccacgc ggagaactgc ttctggagcg
     3661 gcgccgccag ccgcccgagc gcgcccgtgg acgcgtacca ggaggcgggt aagacagcga
     3721 tcaccggcgc gttcgtctcg gcatcgatct cctcaggcgt cgtctgcccg ctgaagtcga
     3781 agctgatgcg ctccgtgaac gagacgcccc acttgaaccg gtacttcccc tctacgaacg
     3841 gatagaggag gtagtggggc aggttcttgc catactccgg accaggttgc gccggcagga
     3901 ggtcgatgga aaggccgttg ccctcgcacg agaggccctt cggccagcgc tgccagaaat
     3961 cgcgcaccgc gacggtcagc gcgccccgct cggtggagac gcgaatcacg cccggcgcct
     4021 ggcctccctt gctctgctga ccatcggcgc gcaccaggca ctgcgcttcg tcctcctgga
     4081 agagcgagag cgtccggccc tcgcgcggct gcagcttccc cgccttgtcg cgcaggaaga
     4141 gcgttccctg ccggatgctc ccggacggcg cgaacggcag cgagagcgag gtgatatcgg
     4201 tgaactcggt cctcaggtag tcgttgagat gcgtcagcgc cagttcaacg cgcggcgagc
     4261 cggcacggaa agtgagccgg gcgatgtagc gcatgtatgt cgtgccatcg gctgccgcgt
     4321 aggcgccctc gacgcgcacc acaagcatgc acgggccctg ctcctcgatc cgcacgctct
     4381 ccggtgggcg agcggaggcc gtgaacagct tgccgcgctc gtcgaccagg cggatcccat
     4441 ccgggccgaa tgccgcgacg cgctcggttt cagcaaaccg gccatcgcct ttggcatcga
     4501 ggcgcacgtc ctgaagcagg ttgaactgcc gcttatcgat caccgcctgc agcggcccgg
     4561 tggtgaccgt cagctgcgcg tcggtctcgg tggctttgag cggggaggac gcggcggcgc
     4621 gcttcacctg gctcccgaac tcgacggtgt actcagtcga ttcgcgcgct ttgagcggtg
     4681 ccgcgaactg gacgagcacc cacttgaggc tgtcatcagg ccaaaaggag gtgggcgtca
     4741 cctggcaggg gatctcagtg ccccctggag agagaatgcg cacctgcgct gcgtcgaaga
     4801 gggcaccctt ggcaagaggg aagccgaagc ggatgggcgt cgcctttcgg gcgacccccg
     4861 cctcttcgag gagatgaacc cgcacgcgct cgcgggtggc cgagccgctc ttccccggct
     4921 ggcgcgccgt ggccgagaat ggcacggcgg ggcgcggccg gccgatggtg atcggcgtct
     4981 ttggcgctac ctgcccgtag gtgaggcctt tgtcctccag gcgctccagc ttcaccacgt
     5041 cgttatgata ccggtagtcg gcaaagccgc gcaaatcggc tagctggcgg cgatccagct
     5101 tgggtaccgc ctgtgccgat cgcggcagca tcgccacccg gtggggcgtg ccgtcactcc
     5161 gcccgccggc gcgctggacg aggagatgga ctccgactgc gatctggtca ccgaccaccg
     5221 ccaggggcgc gtcgaggacc agatagagca cgttcccatc taccgtgccg cctgccatag
     5281 agttcccact gtcataggcg ttgttgtgga gggagaggga gacatctcgc ccagacacca
     5341 ccgccatcag atcgacacca ccgtggtgtg ggtctttgcg ccctgtctgc gggttgttgt
     5401 ccatgtcggc gtagaggatg agggtggcgc cgatgaagtt gggcttgcgc gcgaaggtca
     5461 cccggaagag caggcggttt ccgcccacgt ggcaggcatc gagagcgaga atatcgggcg
     5521 cgtgttgcgc ggcctgctct tcggagtagt tctcattctt gctagtcgcg atagggacca
     5581 gttcctgctt gacctgcggc agcaggccga gctttgtctc tactgcatcc ggaatgccat
     5641 cgccatcggc atccgcggcg ggtgcggagc acgcgggcga taggacgaaa gagagcgccg
     5701 tgagcacgag cgcgaggcgg tatagatgca tggtggccaa ctcctccgtt caagggtacg
     5761 cgggcggggc gctaccccgt tggggaagcc attctacatc atgctagccg atccctggcc
     5821 gggatgctac cccaggagcg agcctgaacg ccggggccga ccggcacgcg ggccaataca
     5881 cgcggcgggt ctccccggcg cccggcacca tgccagagcc cgtcagttcc accgggttcg
     5941 tttagccggg ctcctcttca tttgcagata aataaagacg cggcgtattc ctccagcgga
     6001 atgaggagcc agtagtcgag gttgagcagg ccggagagcg ttaggatctc caggtggtgc
     6061 tcgccggcag tcagctcgat ggcggctggc tggccctggg cgtcgcacac
//
LOCUS       DAOSCV010000131         6082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8238_length_6082_cov_2.981915, whole genome shotgun sequence.
ACCESSION   DAOSCV010000131 DAOSCV010000000
VERSION     DAOSCV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8238_length_6082_cov_2.981915"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLY56_04610"
     CDS             complement(<1..293)
                     /locus_tag="PLY56_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20794.1"
                     /translation="MAAESPIAGEMWPALPPLPEGEWPAQGKWTYEEYQRLPEVPARR
                     FEVVCGVLWTTRAPTVSHETVVHRVAWELEQYQDRHPGDLVLSAPTEVLIPGLA"
     gene            complement(283..789)
                     /locus_tag="PLY56_04615"
     CDS             complement(283..789)
                     /locus_tag="PLY56_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ20795.1"
                     /translation="MQITARIAGHGEVPLLVDLMEEFYAESGYALDREWARASFRHFL
                     SDPSLGRAWVLFDGAEPAGYVVLTVRFAMEYGGLEGFIDDLYVRPAYRRHGIARHALN
                     TLFEECRRWGIRAVQVEVGRGNAAANALYASFNLRPRDDDRVTLVTTLHDRPEEPEGS
                     GKGGGVGC"
     gene            complement(858..1712)
                     /locus_tag="PLY56_04620"
     CDS             complement(858..1712)
                     /locus_tag="PLY56_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010995659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20796.1"
                     /translation="MWRGIRWGAIGLAAVTAGSLTYGRSRWASGTRQVRARLEAAREP
                     IEPAVFHAHGLDGLPAPVQRYFQAALKDGQPVVSAVSVEHTGHFNLGERGENWKPFTS
                     SQRVITRRPGFDWDARVALVPGLAIHVRDAYVAGEGLLYAAPLGLFTVMDVSRQPGVA
                     EGELMRFFAEAAWYPTALLPSQGVRWEAVDGRSARATLADGDLTLTMTFTFNDEGLIE
                     TARAEARGRMVGDTVVPTPWEGRFGEYATRDGMCVPLYGEVAWILPEGPRPYWRGRIT
                     KIAYEFAH"
     gene            1922..4282
                     /locus_tag="PLY56_04625"
     CDS             1922..4282
                     /locus_tag="PLY56_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20797.1"
                     /translation="MEWQTGWHFGGWVMALGVALGATAAAGAEEIRFTVKNPATRPVA
                     TVARVSLPVPKGALPKRPPARVLLGGKVHSAQESVITHHPDGSPRRVILSFPVNLGAG
                     ESVEGRYGSGEAQAAPEEKAPGIIVTDRWQVVPGIDRVELRRTDGTLLAAIEPFGPVK
                     PEGKASAQVIEAGPYFTWLRYDRPGEVWGQQIDVQVHRTGEVRLTHRIQAKPAGDHWT
                     PDFGWRMMAPGARATEPLKPTARFLGRDPNSRFSDEANADLIAAFTLSDSTPVCVANP
                     EALRQNRGTFEVTQADGAIVTRSNRNEPVKDLETEGLMIQEGSWRFSELVVVPGGREE
                     LAARLDAPLFGHASWQAYDAVYRTGPPLEVKHPVLRQCVERFIFALQDMQMKGDDLGS
                     MPWSWSPYRAKPDYTSSVRLNHSLYVWEDWFRTGDYRLWRVAHDWCRNYFDLGMYWGP
                     NPEFYGACRRGNAWRGKSGHGPGTFNPRFANTQIYVHKGWSNFWLMYEETGDPRYRHA
                     AEAAAEWSIKHQHAGLGYTRTVGVVADAVKLYEYTGDRKHLENALRLWETFKPTQGED
                     LLFTESGKPAVGNDLYIGIDSVGYKTPFVKPYIVQYATNALPYLLRHTPGDDRLHRTI
                     LALNDWMARVQQPGGGWGYPHPAAAGPRWNLEYTHGILLAHGVEPKKEYLDAAARILR
                     PIAQLCELHGWPASGLDPWEFRAKINLHQRQEMYKRATDRDPMRDYDEGRVKFDWPPD
                     DVVYFQAVLRDYLAHRPEASLFESDAILEKIKRLPTALPPADARPK"
     gene            complement(4419..4805)
                     /locus_tag="PLY56_04630"
     CDS             complement(4419..4805)
                     /locus_tag="PLY56_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20798.1"
                     /translation="MLEQAVAVTPERKREDAERMLLLGRFIRNCVQTAINTKRWWQLK
                     QAALEEKDPQKAGALLDDLVRLGEAELANVRDTIPLVEADSRLGWEPSMEYMTDRAHL
                     EWKIAQLTRVLEEEIPEYRSKLGSAA"
     gene            complement(4781..>6082)
                     /locus_tag="PLY56_04635"
     CDS             complement(4781..>6082)
                     /locus_tag="PLY56_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20799.1"
                     /translation="KMDAVRAPLFSPRMVHSGWGIDQFPDQHLNAIAHAGMDAILLFV
                     KGPNMTTTGHLDINNLIDRAEAYGIDVYLYSYLISRYHPDTPGALEYYESTYGELIRS
                     CPRAKGLIFVGESCEFPSKDPRTRGWIRKDPPPDAKPDSRPSPGWWPCTDYPQWLDMV
                     KGVLRKYNPDLDIVFWTYNWGWAPEEDRLALIRSLPTDISLQATFEMFENVIKEGIVT
                     RCVDYTASFEGPGKYFRSEAQAAHERGLRLYTMSNTAGLSWDIGVIPYEPIPFQWARR
                     YRGLHEAREKWGLVGLMESHHYGWWPSFVNELAKWAFWAPTPTTEEMADRIARRDFGP
                     EGAPLAVKAWQAWSEAFRDYVPTNEDQYGPFRVGPSYPLLFLEEKEPFPSAPYAHFGA
                     RILTTNYRPTSRRISPPRSSSWSGCAPGGKRASACSSRQSQ"
BASE COUNT         1030 a         1957 c         2100 g          995 t
ORIGIN      
        1 gccaggcccg ggataagaac ttccgtgggc gcggaaagga cgagatcgcc cggatgcctg
       61 tcctggtatt gctccagctc ccaggcgacg cggtgtacca ccgtctcgtg gctgaccgtc
      121 ggcgcgcgag tcgtccagag gacgccgcac acgacctcga agcggcgggc gggcacctcc
      181 ggcaggcgct ggtactcttc gtaggtccac ttgccctggg ccggccactc gccctcggga
      241 agcgggggga gggcaggcca catctcgcca gcgatggggg attcagcagc caacgccgcc
      301 tcctttccca gatccttcgg gttcctcggg cctgtcgtgg agcgtggtca cgagggtgac
      361 gcggtcatcg tcgcgcgggc gcaagttgaa cgaggcatag agcgcgttgg ccgcggcgtt
      421 gccccggccc acttccacct gcaccgcgcg gataccccac ctccggcatt cctcgaagag
      481 agtgttcagg gcgtgccggg cgatcccgtg ccggcggtat gccgggcgga cgtaaaggtc
      541 atcgatgaag ccttccaggc cgccatactc catcgcaaag cgcaccgtca ggacgacgta
      601 gcccgcgggc tccgcgccat cgaaaagaac ccaggcgcgc ccgagcgatg ggtcgctcag
      661 gaagtggcgg aagctcgccc gggcccattc gcggtccaga gcgtagcccg actcggcgta
      721 gaactcctcc atgaggtcga ccagcagcgg gacctcgccg tgcccggcga ttcgggcggt
      781 gatctgcatc cttctcctcg atatccgggg gccggcagga cgccggcacc gcatgttcct
      841 atctactatg cgcgcgctca gtgggcaaac tcgtaggcga ttttggtaat tcgcccgcgc
      901 cagtagggcc ggggcccctc ggggagaatc caagccacct cgccatagag gggaacgcac
      961 atgccgtcgc gcgtggcgta ctccccgaag cggccttccc acggcgtggg taccacggtg
     1021 tcgcctacca tccggccgcg cgcctcggcg cgtgccgtct cgatcaggcc ctcgtcattg
     1081 aaggtgaaag tcatcgtcag cgtgagatcg ccatcggcca gcgtggcgcg cgcgctgcgc
     1141 ccgtccacgg cttcccagcg cacgccctgg ctgggaagga gcgcggtggg ataccaagcg
     1201 gcctcggcga agaagcgcat cagctccccc tcggcgacgc ctggctggcg gctcacgtcc
     1261 atgacggtga acagcccgag gggcgccgcg tagagaagac cctccccggc gacgtaggcg
     1321 tcgcgcacgt ggatcgccag accgggcacc agggcaacac gcgcgtccca atcgaagccc
     1381 ggccggcggg tgatcacgcg ctgagaggat gtgaacggct tccagttctc ccctcgctcg
     1441 ccgagattga agtggccggt gtgctccacg ctgaccgcgc tcacgaccgg ctgcccatcc
     1501 ttcaaagcgg cctggaagta gcgctgtacc ggcgccggca ggccatccaa cccgtgcgcg
     1561 tggaacaccg ccggctcaat cggctctcgc gccgcctcca accgtgcacg tacctggcgc
     1621 gtgccggatg cccagcggct ccggccgtag gtgagcgaac ccgctgtcac ggctgccagg
     1681 ccaatggctc cccatcgaat gcctctccac atgaccacgt ctcccccgct tcgtaacgtt
     1741 cccagcgccc ggccgctgcg cggatccccg acattccatc aaccgatggg cgccatcggg
     1801 cggatcatac acctgaaagg ccaggggtgt caatggaagc ggagggggcg ccaggatcct
     1861 gcgcgggcga gaaggaatca tgggatgccg gatcggaaca tgccggcgag gaggtctagc
     1921 gatggagtgg caaactggct ggcacttcgg aggatgggtc atggcgcttg gggtggcgct
     1981 cggggcgact gcggcagctg gtgcagagga gatacgcttt acggtgaaga acccggctac
     2041 gcgaccggtg gcaacggtgg cgcgggtgtc gctgcccgtg cccaaaggag cgctccctaa
     2101 gagaccgcca gcgcgtgtgc tcctcggggg caaggtgcac tccgcgcagg agagcgtgat
     2161 cacgcaccac ccggatggct ctccccggcg cgtgatcctc agcttccccg tgaacctggg
     2221 cgcgggggag agtgtcgagg gccgctacgg ctcgggcgag gcgcaggcgg cgcctgaaga
     2281 gaaagcgcct ggcataatcg tgaccgaccg ctggcaggtg gtgcccggaa tcgaccgcgt
     2341 cgagctgcgc cggacggatg gaacgctctt ggcggcgatc gagccgtttg gcccggtaaa
     2401 gcccgaaggg aaggccagcg cccaggtgat tgaagcagga ccgtatttca cctggctgcg
     2461 gtatgaccgc ccgggcgagg tgtggggaca gcagatagac gttcaggtgc accggacggg
     2521 cgaggtgcgg ctcacgcatc ggatccaggc gaagccggcg ggcgatcact ggacccctga
     2581 cttcggctgg cggatgatgg cgcccggagc gcgggctacg gagccgctga agccgacggc
     2641 acgcttcttg gggcgtgacc cgaactcgcg tttctccgac gaggccaacg ccgatctgat
     2701 tgccgcgttc acgctgagcg attctacccc cgtttgcgtg gcgaacccgg aggcgctccg
     2761 gcagaaccgg ggcacgtttg aagtcactca ggcggatggg gcgattgtga cccgctcgaa
     2821 ccggaacgag ccggtgaagg acctggagac tgaagggctg atgatccagg aaggctcgtg
     2881 gcggttcagc gagctggtgg tggtgccggg tgggagagag gagctggccg cacggctcga
     2941 cgcgccactc ttcgggcatg ccagctggca ggcgtatgac gcggtgtatc ggacgggccc
     3001 gccgctcgag gtgaagcatc cggtgctgcg ccagtgcgtc gagaggttca tcttcgccct
     3061 tcaggatatg cagatgaagg gcgatgacct cggatcgatg ccgtggtcgt ggtcgcccta
     3121 tcgggcgaag ccggattaca cctcttcggt ccggctgaac cactccctct acgtgtggga
     3181 ggattggttc cgtaccggcg actaccggtt gtggcgcgtg gctcacgatt ggtgccgcaa
     3241 ctacttcgac ctggggatgt attgggggcc caacccggag ttctacggcg cgtgccggcg
     3301 cgggaacgcc tggcgcggca aatcggggca tggtccgggc accttcaacc cgcgttttgc
     3361 gaacacgcag atctacgtcc ataagggctg gagcaacttc tggctgatgt atgaggagac
     3421 gggcgacccg cgctaccggc acgcggcaga ggccgccgcg gagtggtcga tcaagcatca
     3481 gcatgccggc cttggctaca cgcggaccgt cggcgtcgtc gcggacgcgg tgaagctgta
     3541 tgagtacacg ggtgaccgga agcacctgga gaatgccctg cggctatggg agacattcaa
     3601 gcccacgcag ggcgaggacc tcctcttcac ggagagcggc aagcctgcgg tggggaacga
     3661 cctctacatc gggatcgatt cggtggggta taagacgccg tttgtgaagc cgtacatcgt
     3721 gcagtatgcg accaacgcgc ttccctacct gctgcggcac acgccggggg atgaccggct
     3781 gcaccggacg atcctggcac taaacgactg gatggcacgg gtgcagcagc cgggcggggg
     3841 atggggctac ccgcatccag ccgcggccgg cccgcgctgg aacctggaat atacccacgg
     3901 gatcctcctg gcgcatggcg ttgagcccaa gaaggagtat ctggatgcag ccgcgcgcat
     3961 cctgcggccc atcgcgcagc tgtgcgagtt gcacggctgg ccggcatccg ggctggatcc
     4021 gtgggagttt cgcgccaaga tcaacctgca ccaacgccag gagatgtaca agcgtgcgac
     4081 cgatcgcgac ccgatgcgag actacgacga aggccgggtg aagtttgact ggccgcccga
     4141 tgacgtggtc tatttccagg cggtcctgcg cgactacctg gcacaccgcc cggaggcgtc
     4201 gctcttcgag agcgacgcga tcctggagaa gatcaagcgc ctgccaactg cgctcccgcc
     4261 cgcagacgcc cggcccaagt gagccgttcc ggatagaact cccatccgga ttgtccgggt
     4321 ggggaggggg aggcccgcag ggcctttgct gggtagcagc gcggagcttc gagctccgcg
     4381 cgtggggatg cggcctcccc cttcatccat tcagccggct aagccgctga acccagcttg
     4441 ctgcggtact cgggaatctc ttcctccagc acgcgcgtga gctgggcgat cttccactcc
     4501 aggtgggcgc ggtcggtcat gtactccatg ctcggctccc accctaggcg cgaatcggct
     4561 tcgaccagcg ggatagtgtc gcgaacgttg gcgagctcgg cctcgcctag gcggaccagg
     4621 tcgtcgagga gggcgccggc cttctgcggg tccttttcct caagcgccgc ctgcttgagc
     4681 tgccaccacc gcttggtgtt gatcgctgtc tgtacgcagt tgcggatgaa ccggcccagg
     4741 agaagcatgc gctcggcatc ctcgcgcttg cgctccggcg tcactgcgac tgcctgctcg
     4801 agcaagccga ggccctcttc ccaccgggtg cgcatccgct ccaggaggaa gatctcggtg
     4861 gggagatcct ccggcttgtc ggccggtagt tcgtggtaag gatccgggcg ccaaagtggg
     4921 catagggcgc ggacgggaac ggctccttct cctcgaggaa gagcagggga taggacgggc
     4981 ccacgcggaa ggggccgtat tggtcctcgt tcgtgggtac gtagtcgcgg aacgcctcgc
     5041 tccaagcctg ccacgccttt accgccaggg gcgcgccctc cggaccgaag tcgcgccgcg
     5101 cgatccgatc ggccatctcc tcggtggtgg gggttggcgc ccagaaggcc cacttcgcca
     5161 gctcattcac gaacgaaggc caccagccgt agtggtgcga ctccatcagc ccgaccaggc
     5221 cccacttctc acgcgcctca tggagccccc ggtagcgccg cgcccactgg aacgggatcg
     5281 gctcgtaggg aatgacgccg atatcccacg agagccccgc cgtattgctc atcgtgtaga
     5341 gccggaggcc gcgctcatgc gccgcctgcg cctccgaccg gaaatacttg cccggcccct
     5401 cgaacgacgc ggtgtaatcc acgcagcgcg tgacgatccc ttccttgatc acgttctcga
     5461 acatctcgaa ggtggcttgc agcgagatgt cggtggggag cgagcggatc agcgccagcc
     5521 ggtcctcctc gggcgcccat ccccagttgt aggtccagaa gacgatgtcg aggtccgggt
     5581 tgtacttgcg gaggacgccc ttcaccatgt cgagccactg gggatagtca gtgcagggcc
     5641 accagccggg gcttgggcgg gaatccggct tggcatcggg cggcggatcc ttgcggatcc
     5701 acccgcgggt gcgcggatct ttgctgggga actcgcacga ctcgcccacg aagatgagcc
     5761 ccttcgcccg cgggcacgag cggatcagct cgccatacgt gctctcgtaa tactccagcg
     5821 ctccgggagt atcgggatgg tagcggctga tcaggtagct gtagaggtaa acatcgatcc
     5881 cgtaggcctc ggcgcgatcg atgaggttgt tgatatcgag gtggcccgtg gtggtcatgt
     5941 tcgggccctt gacgaagagc aggatcgcgt ccatgccggc atgcgcgatg gcgttgaggt
     6001 gctgatcggg gaactggtcg atgccccagc ccgagtgaac catgcgcggc gagaagaggg
     6061 gggcgcggac cgcgtccatc tt
//
LOCUS       DAOSCV010000132         6067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8264_length_6067_cov_3.674152, whole genome shotgun sequence.
ACCESSION   DAOSCV010000132 DAOSCV010000000
VERSION     DAOSCV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8264_length_6067_cov_3.674152"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLY56_04640"
     CDS             <1..309
                     /locus_tag="PLY56_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ20800.1"
                     /translation="VLPTLGDTFAQVILEAMASGLPVVASRVGAIPELVVEGETGFLV
                     PPGDADALAQAVNLLLDESGLRERMGRAGWQRAREEFDIARNGRRLVDLALRAREEAR
                     "
     gene            306..1073
                     /locus_tag="PLY56_04645"
     CDS             306..1073
                     /locus_tag="PLY56_04645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ20801.1"
                     /translation="MRRITLPGWRVPILMYHRVTPAEPGPGDYFFSISRRRFAQQMHY
                     LQRLRFHVVTLEQIVDWLRRGRALPPRPVAITFDDGYADTYRLAVPILRELRFPATFF
                     LVSDHVGGTSRWEAESGCAGQPLIGWEEARDLARQGFTIGSHTRSHAVLTTLSDGCLR
                     AELLESRVIIERQVKTPIRFFAYPRNRTDARCQEAVRAAGYEGACAGDGLGYHPWCLD
                     RVDVSHASPAAFLAKVSPWFYAFRRARIRVTGSLQHP"
     gene            1152..1619
                     /locus_tag="PLY56_04650"
     CDS             1152..1619
                     /locus_tag="PLY56_04650"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HOJ20802.1"
                     /translation="MAEENANTSRRRAGWILLAAGVAGAAMVWAHRRYFTVRAVQTGE
                     SPEYPDLVPQVFAYPADEVWNTALEAVRTLPGWSVVAEEDGVIHATVAAAAHPHEVSI
                     SIEPLMNGRMMKVTASSRCQGRMPDLGANARIVRAFQARLRELLPSGGETRVQ"
     gene            1609..2391
                     /locus_tag="PLY56_04655"
     CDS             1609..2391
                     /locus_tag="PLY56_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HOJ20803.1"
                     /translation="MSSEARPVALVTGASRGIGRGIALELARTGYDVLVNYASNAEAA
                     RAVQAEIAALGARAEIFGASIADSDARQALVDFTLDTFGRIDLLVNNAGIAPRQRRDL
                     LEATEESYDEVMGTNLKGPYFLTQRVANEMIRLLRDKQIPRGRIVFVTSMSAYTSSPN
                     RGEYCLSKAGLSMAVALYADRLAEFGIPVLEVRPGIIATDMTSVVKERYDRLIGEGLL
                     PLRRWGQPEDIGRAVAAIARGDFDYSTGTVINVDGGFCLRRL"
     gene            complement(2482..5769)
                     /locus_tag="PLY56_04660"
     CDS             complement(2482..5769)
                     /locus_tag="PLY56_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOJ20804.1"
                     /translation="MPQSRWFSWLPRAATCFALILFWQAAPRPSERPTLVPQMGHGDL
                     VTGIAFSPDGHTLASASEDRTIILWDVASGAARLSLKGHSSWVQCVAFSPDGRTVASG
                     SEDDTVILWDASTGAVRHVLKGHAADVTSVLFSPDGRLVASRAEDRTVRLWDAETGMA
                     MATIPGVFSRVAISPNGQFLAFANRQHIITLWDIAAGKPARTFQSHRTSIASLAFSQD
                     GQWLASGNDDGGVTLWNAATGQPARSLKGHADAVTSLAFSRNGRWLVSGSSDAAILWN
                     TATGRARRTVESDARAVALSPDGHLLACSDGATVVLRDTRSGKVQRTLQGQAQEVARV
                     AFNPDGQTLAAATAAGTVLWDIENAEARATLRGHEAGVTAAVFSPDGQTLATASQDRN
                     VILWDLDAREPRLVLKGHTESITQVAFTPDGKRLASASADGSIFLWNTETGEQLATLK
                     GHTWPVAGLAFSADGHTLASGSWDTTVILWDTQGGEQRRRLQGHQGWVHSVAFSPDGR
                     VLASAGMDRRIVLWDVETGQALATLSGHAAPIRDVAFSLDGKALLSGDEGGTLLVWNV
                     ATREVRHTLHGHGALVSSVTFSPNGRVIASASWDGSVRFWRADTGAELAAAFSLEGGR
                     DWLVATPQGYYNASLDGGRLVRWRVGEALYPVEQYARRFKKPDLVARALGGATLGGRL
                     PVLTAESRPPRVTLTAAGGKSEVQGDRLRVQIEAAPGTPDRSITRVELRVNGRLAELD
                     RALDVAIASQGKGKQASSVAALGKTRSVRARPRDRAADSRLSIDAVISLLPGEEEIRL
                     LASAYDDQEVRGDSELVVLRRISPAPAADTDCYVLAIGVSRYSTPEYNLSFADDDARA
                     VAATFQAQQGHLFRKVEAKVVTDDHGTKKEIETALKWLQKQPRPGDTAVMLFSGHGVR
                     GHLRRLFFFTHNGDLDALRDTCLPWDAVDDAVSACRARQVLLFIDCCHAGAFGERRAS
                     ADEMAENLMRNVVVFASSRGNEESLEDPTWGHGAFTRALLDGLHGGADFDESGFVTAG
                     DLVRYLPDRVRELTGGRQNPIVPRPEELDPALRLAYLKRDEPAAARSHDNWYPRAALA
                     GPRRAGE"
BASE COUNT          917 a         2043 c         2096 g         1011 t
ORIGIN      
        1 gtgctcccga cgttgggcga tacctttgcg caggtgattc tggaggcgat ggcgagcggg
       61 ttgccggtgg tcgccagccg cgtgggcgcg attccagagc tcgtggtgga gggtgaaaca
      121 ggcttcctgg tccccccggg agatgccgat gcgctggccc aggccgtgaa tctgcttctg
      181 gatgagagtg gcctacgcga gcggatgggc cgagcggggt ggcagcgtgc tcgagaggag
      241 ttcgatatcg cgcgcaacgg gcgccgcctc gtcgatctgg cactgcgcgc gcgggaggaa
      301 gcgcgatgag gcggatcacg cttcccgggt ggcgcgtgcc gatcctcatg taccaccgcg
      361 tcacccccgc cgagccgggg ccgggcgact acttcttcag catctcgcgc cggcgcttcg
      421 cgcagcagat gcactatctg cagcggctcc gcttccatgt cgtcacactc gaacagattg
      481 tcgactggtt gcgcagggga agggcgttgc cgccgcgccc ggtggccatc acctttgatg
      541 atggctacgc cgacacctac cggctggcgg tgccgattct ccgggagctc cgcttccctg
      601 ccaccttttt cttggtgagc gaccacgtgg gcggtacttc ccgatgggag gcggagtccg
      661 gatgcgccgg ccagcctctg atcggctggg aagaggcgcg cgacctggcc cggcaaggct
      721 tcaccatcgg ctcgcataca cgctcgcacg ccgtcctcac gacgttgagc gatgggtgcc
      781 tccgcgcgga gctgctggaa tcgcgggtga tcatcgagcg gcaggtgaaa accccaatcc
      841 gcttcttcgc ctacccgcgc aaccgcaccg atgcccgctg tcaggaagcg gtccgcgccg
      901 ccggatacga gggcgcgtgt gccggtgatg gccttgggta tcatccctgg tgcctggacc
      961 gggtggatgt atcgcatgcg tcgcccgccg ccttcctggc caaagtgtcg ccgtggttct
     1021 acgccttccg gcgcgcgcgc atccgcgtga ctggctcact gcagcacccc tgaaggagga
     1081 cgcggtagca tcgcgaactc tggatcggtg cggcggatag cccgcgtctt gtcggcaagg
     1141 ggaagggaac catggctgaa gagaacgcaa atacctctcg acgacgggca ggctggatcc
     1201 tactggcggc aggtgttgct ggagcggcga tggtgtgggc gcaccggcgc tacttcaccg
     1261 ttcgcgccgt gcagacgggg gagagccccg aatacccgga tctcgttccc caggtgttcg
     1321 cctatcccgc cgacgaggtg tggaatactg ccctggaagc agtccgcacg ctccccggat
     1381 ggagcgtggt ggcggaagag gacggcgtga tccatgccac cgtcgcagct gccgcccatc
     1441 cccacgaggt gagcatctct atcgagccac tgatgaacgg acgcatgatg aaggtgacgg
     1501 cctcctctag atgccagggg aggatgccgg accttggtgc caatgcccgc atcgtgcgtg
     1561 ccttccaggc acgcctgcgc gagcttctgc cttcgggagg agagacccgt gtccagtgaa
     1621 gcaagaccgg ttgccctcgt caccggtgcc agtcgtggca tcggccgggg tatcgccctg
     1681 gagttggcgc gcaccggcta cgacgtgctc gtgaactacg ccagcaacgc cgaggccgcg
     1741 cgtgcggtgc aggcggagat tgccgcgctc ggcgctcgcg ccgagatttt cggcgccagt
     1801 atcgccgatt ccgatgcgcg gcaggcgctt gtcgatttta ccctggacac cttcggccgg
     1861 attgacctcc tggtgaacaa tgccgggatc gcgcctcgcc agcggcgtga ccttctcgaa
     1921 gcgaccgagg agagctacga cgaggtgatg ggcacgaacc tgaaaggccc ctatttcctc
     1981 acgcagcgcg tcgccaacga gatgatccgc cttctgcggg ataagcagat cccccggggc
     2041 cgcatcgtct tcgttacctc gatgtcggcc tacacctcct cgccgaaccg cggcgagtac
     2101 tgcctctcga aggcgggatt gagcatggcc gttgccctct atgccgaccg gctcgcggag
     2161 ttcggcatcc ccgtccttga ggtgcgccct ggcatcatcg ccaccgatat gacgagcgtg
     2221 gtgaaggagc ggtatgaccg cctgattggc gagggattgc tgcccctgcg ccgctggggc
     2281 cagccggaag atatcgggcg ggccgtcgcc gccatcgctc gcggagactt tgactactcc
     2341 accggcaccg tcatcaacgt ggacggcggt ttctgcctca ggaggctcta gagctttcac
     2401 tccccacggg agaccccata tacttgtagg ccccgcctcg atcgaggcgg ggcctcttcg
     2461 taactcgcgc gagttcctcg cctactcgcc tgcccggcga ggtccggcca gcgccgctcg
     2521 cgggtaccag ttgtcatggc ttctggccgc agccggctcg tctcgcttca ggtaggccag
     2581 gcggagggcg ggatctagct cttccgggcg gggcacgatc gggttttgcc ggccgccggt
     2641 gagctcgcgg acgcgatccg gcaggtagcg gaccaggtcg cccgcggtga cgaaccccga
     2701 ttcatcgaag tcggcgccac catgcaggcc gtccagcagg gcccgggtaa aggcgccatg
     2761 cccccaggtc gggtcttcca gcgactcctc attgccgcgg ctggacgcaa agacgactac
     2821 gttgcgcatc agattctcgg ccatctcgtc cgcgcttgcc cggcgctcgc cgaaagcgcc
     2881 ggcgtggcag caatcgatga agagcagcac ttgccgggcc cggcacgcgc tcacggcgtc
     2941 atcgacggcg tcccacggca ggcacgtatc gcgcagcgcg tccaggtcac cgttgtgcgt
     3001 gaagaagaag agccggcgca ggtgcccgcg caccccgtgg ccgctgaaga gcatcaccgc
     3061 ggtgtcgcct ggcctgggct gcttctgtag ccacttcagc gccgtttcga tctccttctt
     3121 ggtcccgtgg tcatcggtga ctacctttgc ctccactttg cggaagaggt gcccctgctg
     3181 tgcctggaaa gtggcggcga cggcgcgcgc gtcgtcatcg gcgaaactga ggttatactc
     3241 cggcgtggag tagcggctca ccccgatcgc gagcacgtag caatcggtgt ccgcggccgg
     3301 cgccgggctg atccggcgca acacgaccag ctcggagtcg ccgcgtacct cctggtcatc
     3361 gtaggcgctg gcgagaaggc gaatctcctc ctcgcccgga agcagcgaga tcacggcatc
     3421 gatggagagg cggctatccg ccgcgcggtc gcgggggcgc gcgcgcaccg accgtgtctt
     3481 gccaagggcg gccacggagg aagcctgctt gccttttccc tgggatgcga tggccacatc
     3541 gagcgcccgg tccagctccg ccagccggcc attcacgcgg agctctacac gcgtgatgct
     3601 gcgatctggc gtgcccggcg ccgcctcgat ctgcacgcgg agccggtcgc cttgcacctc
     3661 gctcttcccg cccgcggcag tgagtgtgac gcgagggggc cggctctcgg cggtgagcac
     3721 cgggaggcgc ccgccgagcg tcgcgcctcc aagcgcacgt gccaccaggt ccggcttctt
     3781 gaaccggcgg gcatactgtt cgacagggta gagcgcctcg ccgacgcgcc agcggaccag
     3841 gcgcccgccg tcgagcgagg cgttgtagta gccctgcggc gtcgccacca gccagtcgcg
     3901 cccgccctcc aggctgaagg cggcggcgag ctccgcgccg gtatctgccc gccagaagcg
     3961 caccgagcca tcccaactcg ccgaggcgat cacccgtccg ttggggctga acgtgacgct
     4021 gctgaccagg gcgccgtggc cgtgaagcgt gtggcgcacc tcgcgcgtcg caacgttcca
     4081 gacaaggagc gtgcccccct cgtctcccga gagaagcgcc ttcccatcca ggctgaaggc
     4141 gacatcccgg atgggcgcgg cgtgcccgga gagcgtggcg agggcctgcc ccgtctcaac
     4201 atcccacagg acgatccgcc ggtccatgcc cgcggatgcc agcacacggc catccgggct
     4261 gaacgccacg ctatgcaccc aaccctggtg gccctggagg cgccggcgtt gctcgccgcc
     4321 ctgggtatcc cacaggatca ccgtggtatc ccagcttccg gaggcgaggg tgtgcccgtc
     4381 ggcgctgaac gccaggccgg ccacgggcca ggtgtgccct ttcagcgtgg cgagctgctc
     4441 gcccgtctcc gtgttccaca ggaagatgct gccgtcggcg ctcgcggaag cgagcctctt
     4501 gccatccggc gtgaaagcga cctgcgtgat gctctcggtg tggcctttca ggaccaggcg
     4561 cggctcgcgc gcgtccagat cccagaggat cacgttgcgg tcctgactcg cggtggcgag
     4621 tgtctgcccg tccggcgaga agaccgcggc tgtcacgccg gcttcatggc cccggagcgt
     4681 ggcgcgcgcc tcggcgtttt cgatatccca cagcacggtg cctgcggcgg tggcagcggc
     4741 gagggtctgg ccgtcgggat tgaacgccac tcgggccacc tcctgggctt gcccctggag
     4801 ggttcgctgg accttaccag atcgggtatc gcgcaagacg accgtggcgc cgtcgctgca
     4861 cgccaggagg tggccgtccg ggctgagagc gacggcgcga gcgtccgact cgaccgtgcg
     4921 ccgtgcccgg cccgtggcgg tgttccagag gatcgccgcg tccgagctac cggagacgag
     4981 ccagcgcccg ttccggctga acgccaggct ggtgacggca tcggcgtgcc ccttgaggga
     5041 gcgcgcgggc tggcctgtgg ccgcgttcca gagcgtcacg cccccatcgt cattcccgga
     5101 cgcgagccac tgcccgtctt ggctgaacgc caggctggcg atggatgtgc ggtgcgattg
     5161 gaaggtgcgc gccggcttcc ccgcggcgat atcccacagg gtgatgatgt gctgccggtt
     5221 cgcgaacgcc aggaactggc cgttggggct gatggcgacg cggctgaaga cgccaggaat
     5281 cgtggccatg gccatgccgg tttcggcatc ccagaggcgc accgtgcgat cctcggcgcg
     5341 cgaggcgacg agccggccat ccgggctgaa gaggacgctg gtcacgtcgg ctgcgtgccc
     5401 cttcaggacg tgccggaccg cgcccgtgct cgcgtcccag aggatcacgg tgtcgtcctc
     5461 gctgccggag gcaacggtcc ggccatccgg gctgaaggcg acgcactgca cccagctgga
     5521 atggcctttc agagagagac gcgcggcgcc ggaagcgaca tcccagagga tgatggtgcg
     5581 gtcctcgctg gcggaggcga gggtgtgccc gtcggggctg aacgcgatcc cggtgacgag
     5641 atcgccgtga cccatctggg gcacgagcgt cgggcgctcc gaggggcgcg gcgcggcctg
     5701 ccagaagagg attagcgcga agcaggtggc agcgcggggg agccacgaga accatcgact
     5761 ctggggcatg ccggacaatc cttccgtggg gcggagaccg ggagacccgg atccgcgcgt
     5821 tctccctcca ttatagcata accccggcgc ctcgtgtgct cgcgggcgtg ttgttatccg
     5881 cagatggacg cagatggaca cggatggaga aggccgcccg tacgcgcggt gatttatcgc
     5941 cgcgcgccgg gttgcgcgtc accttcgcgc gcggagctcg aagctccgcg ctgcttgtag
     6001 gcaaagcccc tccggggctt ctcctcccag ccaccgccgt tttgagacat ctgggagtat
     6061 ccccaac
//
LOCUS       DAOSCV010000133         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8274_length_6063_cov_4.044441, whole genome shotgun sequence.
ACCESSION   DAOSCV010000133 DAOSCV010000000
VERSION     DAOSCV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8274_length_6063_cov_4.044441"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLY56_04665"
     CDS             complement(<1..273)
                     /locus_tag="PLY56_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20805.1"
                     /translation="MRYRLTSTLGLLLAAVLGMLSAPGARADVTLLNTSYDPTRELYR
                     EFNRAFTRYWKQKSGQRVSIRQSHGGSGKQARAVIDGLEADVVTLAL"
     gene            842..1984
                     /locus_tag="PLY56_04670"
     CDS             842..1984
                     /locus_tag="PLY56_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ20806.1"
                     /translation="MHCFARLPVCFVRGEGARLWDTEGKEYLDFLAGIAVCALGHCHP
                     RVTEAIREQAGRLVHTSNLYYNAEQARLAQLLGELSGGYKAFFGNSGAEANEGAIKLA
                     RKWGKLNRGPECVTIFTALNSFHGRTLLTVSATGQEKVKKNFDPLMPGFVHVPLNDLA
                     ALEAAVDENTLAVMLELVQGESGIHPCDPEYVKAVRRLCDERKMLLILDEVQCGLGRT
                     GAFFAWQRYGVTPDIFTLAKPIANGVPMGVVMARPGVAEAFQPGDHGSTFGGTHLACA
                     AATAVLETMRDERLPERAEEMGRYFMGRLNALREKHAKIKEVRGLGLMVGMELQVPEA
                     KAVMLRCLENGLVINATGDTVLRFLPPLIVTRDEIDRAVEILDDSL"
     gene            2008..3024
                     /locus_tag="PLY56_04675"
     CDS             2008..3024
                     /locus_tag="PLY56_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOJ20807.1"
                     /translation="MRERVAMWEQFTDRTSRALFFAYAEAARLGAERVGAQHLLLGLA
                     RDRDSVATRVLGRLGLPGARVRQEMVKRLAAPQDAPGGEIALDPGVNEVFNLALEEAR
                     ALGYDAIGPEHLLLGILRHGQSEAARLFADWGIDLENARREVVYFASGGHDNPLEIPG
                     WQRFRARARRVISLAQDEALAFGQEKVAPEHLLLGLIREEDCFAARLLTRLGADLDRI
                     REDVEARLGQRGHGSAKNVELEESARRVLSAAVEEAWSDFESDVGTEHLLLGLIREGE
                     GIVADTLGAAGVSLERARQETVAALSGFASLRTVVRQRVAESLGRQRGPKKAQASEEQ
                     PGTT"
     gene            3021..3179
                     /locus_tag="PLY56_04680"
     CDS             3021..3179
                     /locus_tag="PLY56_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20808.1"
                     /translation="MTGLGVGRCWVAWASALSACQAWGRLSSPWEGKEGATLGVAGTG
                     DGVAIEEE"
     gene            3274..4212
                     /gene="argF"
                     /locus_tag="PLY56_04685"
     CDS             3274..4212
                     /gene="argF"
                     /locus_tag="PLY56_04685"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ20809.1"
                     /translation="MSDALRGRCLTSIADLSLADLGLILNTATALKELLKRTPSVMPP
                     LLRGKTLAMVFEKPSLRTRVSFETGMTQLGGHAINLQPAEIQLGKRESIGDAATTLGR
                     ICDGIMARTFAHETVLQLAAHAGVPVINGLSDREHPCQALADFMTIREKLGEPRGLAI
                     TWVGDGNNVCHSNLLLAAKFGAHFTVACPEGYEPDAEILETAQTIARESGSRIAVTHD
                     PRAAVKDADVIFTDVWASMGQEAEQAERARAFEGFQVNQALVELAKPESIVLHCLPAH
                     RGEEITDEVIDGPHSVVFDEAENRLHAQKAVLVLLM"
     gene            4292..5485
                     /locus_tag="PLY56_04690"
     CDS             4292..5485
                     /locus_tag="PLY56_04690"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOJ20810.1"
                     /translation="MKKIVLVYSGGLDTSVCIPMVREDYGYDHVITVTVDVGQPAEDI
                     RQAEAKARALGTEHYTVDAKDEFVRDYIFPSIRANGDYMGYPISTSIARPLIGLKAVE
                     IAKRLKADAFCHGCTGKGNDQFRIEFPMRAFCPEMPIIAPMREKNLTRSEEIEYAKQK
                     GVPISQSVEKIWSIDENIWGRSIEGGRLEEPDFAPPEEIFQWTKGHDASPNTPRRISI
                     TFEDGVPVALDDERMGGLALVQRVNAIAGEYGVGRIDIMEDRMLGLKVRENYECPAAV
                     TLLTAHRALEALVLTRPELDMKAIVDRKWSELAYQGLWFDPLKEDLDAFIAATQRRVT
                     GTVFLELFKGHAKVIGRRSPWALYSEDLASFDSKSFDQREAVGMVKNHGMQSRMYLQL
                     KSKLA"
     gene            5589..>6063
                     /locus_tag="PLY56_04695"
     CDS             5589..>6063
                     /locus_tag="PLY56_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOJ20811.1"
                     /translation="MRTLWSHGEERPAEVLAEEWGASFAFDARLLPYDVRGSIAHARM
                     LGATGIITPEESARIVAGLEEILREWEADPTVPVTEYEDVHTYVEARLTEKIGETARK
                     LHTARSRNDQVALDARLFVKDAAGEVREGIRHFQRVLVERAEELADVILPGYTHLQ"
BASE COUNT         1030 a         1878 c         2137 g         1018 t
ORIGIN      
        1 caaggccagc gtcaccacat cggcttcgag tccatcgatc acggcgcggg cttgcttgcc
       61 ggagccgccg tgcgactggc gtatcgacac gcgttgcccc gacttctgct tccaatagcg
      121 cgtgaaagcc cggttgaact cgcggtataa ctcgcgggtt ggatcgtagg aggtattcag
      181 gagcgtcaca tcggcgcgcg cgcccggggc agatagcatc cccaggaccg cggccaagag
      241 caggccgagg gtgcttgtca gtcggtatcg catctctctc ctcgtgcgtc caaggcgttc
      301 gaccgcgttt cggtgaagcc cgcagcctgg cctattgtat cacccatcat cccggttccg
      361 ccttcctctt caggccagac attagcagaa tcgggccaca tctggcctca ccggggcccg
      421 gttgttttgc ttgacaccct tcttcgtttc gtgtagaatc gacctgtagg caggagaacc
      481 ggcgggagta tgccttccgg tgaaagcggg tggcgccggt cgcctccgtc attcctccag
      541 actggtacgg tttgcccgac acccctggcc aatctcgtgg tgtcatgcct ctccgtgccg
      601 gcgatgatcc cgtgctgctc cgagttctgg tggggccggg ctgcgggctg ggtgtgccaa
      661 gacactcgga tgaggagcag ctccctccga atggcgcctg ggttcctctt atcaacgggc
      721 acttgtgacc tttggcggag gtgacgcggg cagagggcgt tgttcggata gatgccgaga
      781 taaggagaca ggtacgttgt ccacgttgca ggagatccag gagcttgata gcgcccacgt
      841 catgcattgc tttgcccggc tgcccgtctg cttcgtccgt ggcgaggggg cccggctgtg
      901 ggatacggag ggcaaggagt atctggattt cctggcgggg atcgcggtct gcgccctggg
      961 ccactgccac ccgcgggtga cggaggcaat ccgcgagcag gccggccggc tagtccacac
     1021 ctccaacctc tactacaacg ccgagcaggc gcgccttgcg cagctcctgg gcgagctttc
     1081 cggcggatat aaagcgttct tcggaaactc gggcgctgag gcgaacgagg gcgcgatcaa
     1141 gctggcgcgc aaatggggca agctgaaccg cggcccggag tgcgtcacga tcttcaccgc
     1201 gctcaactcg ttccacgggc gcacgctcct caccgtctcc gcaaccggcc aggagaaggt
     1261 caagaagaat ttcgatccgc tgatgcccgg tttcgtgcac gtgcccctga acgacctggc
     1321 ggcgctggag gccgcggtgg atgaaaacac cctggcggtg atgctggagc tcgtgcaggg
     1381 cgagagcggg atccacccgt gcgatccgga gtatgtgaag gcggtccgcc gcctgtgcga
     1441 cgagcggaag atgctcctca tcctggatga ggtgcaatgt ggcctgggcc ggacgggcgc
     1501 atttttcgcg tggcagcgct acggcgtcac gccggatatc ttcacgctgg caaagccgat
     1561 tgccaacggc gttccgatgg gcgtggtgat ggcgcgcccg ggcgtggccg aggcgttcca
     1621 gccgggcgac cacggcagca ccttcggggg cacgcacctg gcgtgcgccg cagcgacggc
     1681 ggtgctcgag acgatgcgcg acgagcgcct gccggagcgc gcggaggaga tggggcgcta
     1741 tttcatgggc cggctgaacg ccttgcgcga gaagcacgcg aagatcaagg aagtgcgcgg
     1801 cctcggcctg atggtgggca tggagctcca ggtgccggag gcaaaagcgg tgatgctccg
     1861 ctgcctcgag aacggcctgg tgatcaatgc gacaggcgat accgtcttgc gcttcctgcc
     1921 gccgctaatt gtgacgcgag acgagatcga ccgggcggtc gagatcctcg acgactcgtt
     1981 atgaccgttt gatggctcgc cttgctgatg agagagaggg ttgccatgtg ggagcagttc
     2041 accgatcgca ccagccgggc gctcttcttc gcgtatgcgg aggcagcgcg cttgggcgcc
     2101 gagcgagtgg gcgcgcagca ccttctcctc ggcctggcga gggatcgcga tagcgtcgcc
     2161 acgcgcgtac tgggccggct gggcctccct ggcgcccggg tgcgccagga gatggtcaag
     2221 cggctcgcgg cgccccagga tgcgccaggc ggcgagatcg cgctcgatcc gggcgtaaac
     2281 gaggttttca acctggcgct ggaggaggcg cgggctcttg gctacgacgc gatcggcccg
     2341 gagcacctgc tcctcgggat cctccgccac gggcagagcg aagccgcgcg gctcttcgcc
     2401 gattggggga tcgacctgga gaacgcccgg cgcgaggtcg tctacttcgc cagcggcggg
     2461 cacgataacc cgctggagat ccccgggtgg cagcggttcc gggcgcgcgc ccgccgggtg
     2521 atctcgctcg cgcaggacga ggcgcttgcc tttggccagg agaaggtggc cccggagcac
     2581 ctgctcctcg ggctgatccg cgaggaggac tgcttcgcgg cccggctgct tacgcggttg
     2641 ggcgcagatc tggaccggat ccgcgaggat gtcgaggccc ggcttggcca gcggggacat
     2701 ggctcggcga agaatgtgga gctggaggaa tcagcgcgcc gcgttctgag cgcggccgtt
     2761 gaggaagcct ggagcgattt cgagagtgac gtcggcacgg agcacctcct cctcgggctc
     2821 atccgggagg gcgaggggat cgtcgccgac acgctcggcg ccgcgggggt gagcctggag
     2881 agggctcgcc aggagacggt ggctgctttg agcgggtttg cctcgctgcg caccgtggtc
     2941 cgccagcgag tggccgaatc gctggggcgg cagcgcgggc ccaagaaggc ccaggcgagt
     3001 gaggagcaac ctggcacgac gtgaccggcc tgggcgtggg tcgctgctgg gtggcatggg
     3061 cgtctgccct gtcggcgtgc caggcctggg gacggctctc gtcgccatgg gaagggaagg
     3121 aaggcgccac tctgggagtg gccggcaccg gcgatggagt cgccatcgag gaggagtagg
     3181 caagtgtctc ttgagcgcgc gctgaaggaa ctggaaagtg ctatcgctgg cctgcacgcg
     3241 gcattggccg agtcgggtga ggggaagttt cacgtgtcgg atgccctccg gggccggtgc
     3301 ctgacgtcga ttgccgacct gtcgctcgcc gatctggggc tgatcctgaa cacggcgacc
     3361 gccttgaagg agctgctcaa gcggacgccg tcagtgatgc ccccgctcct gcggggcaag
     3421 acgctcgcga tggtctttga gaagccctct ctgcgcacgc gcgtgagctt cgagacaggg
     3481 atgacgcagc tcgggggcca cgcgatcaac ctgcagccgg cggagatcca gctgggcaag
     3541 cgtgagagca tcggcgatgc cgccaccacc ctcggccgca tctgcgatgg gatcatggcg
     3601 cgcaccttcg cccacgagac ggtgctgcag ctcgccgcgc acgccggcgt gcccgtgatc
     3661 aatgggctga gtgaccgcga gcatccctgc caggcgctgg ccgatttcat gacgatccgg
     3721 gagaagctgg gcgagccgag gggccttgcg atcacctggg tgggcgatgg caacaacgtc
     3781 tgccactcca acttgctcct cgccgccaag tttggggcgc acttcaccgt ggcttgcccc
     3841 gaaggctacg agccggacgc ggagatcctg gagaccgcgc agacgattgc ccgggagagc
     3901 ggctcgcgca tcgccgtgac gcacgaccca cgcgcggctg tgaaagacgc ggatgtgatc
     3961 ttcacggatg tttgggcgag catgggccag gaagcagagc aggcagagcg cgcccgcgcc
     4021 ttcgaggggt tccaggtgaa tcaggccctt gtggagctcg ccaagccaga gagcatcgtc
     4081 ctccactgcc tgccggcgca ccgcggcgag gagatcaccg acgaggtgat cgatggcccg
     4141 cactccgtcg tcttcgatga ggcggagaac cgcctccatg cgcagaaagc ggtgctcgtg
     4201 ctactgatgt gaagtctacc tcggatagcc ggaagggaag gcccgagcgg ctgttcgcac
     4261 tctgccgtcc tctatggact ggggggttcc tgtgaagaag atcgtgcttg tctatagcgg
     4321 aggattggat acctccgtct gcataccgat ggtccgcgag gactatggct atgatcacgt
     4381 gattaccgta acggtggatg tgggccagcc ggcggaagat attcgccagg cggaggccaa
     4441 ggcgcgcgcg ctaggcaccg agcactacac cgtggatgcc aaggacgagt ttgtccgcga
     4501 ttacatcttc ccatcgatcc gcgccaacgg cgactacatg ggctatccga tcagcacgtc
     4561 gatcgcgcgg ccgctgattg gcctgaaggc ggtggagatc gcgaagcgcc tgaaggcaga
     4621 cgccttctgc cacggctgca cggggaaggg gaacgaccag ttccgcatcg agttcccgat
     4681 gcgcgcgttc tgcccggaga tgccgattat cgccccgatg cgcgagaaga acctcacgcg
     4741 ctccgaggag atcgagtacg cgaagcagaa gggcgtgccc atcagccaga gcgtggagaa
     4801 gatctggtcc atcgatgaga atatctgggg ccgctccatt gagggcgggc gcctggaaga
     4861 gcccgacttc gccccgcccg aggagatctt ccagtggacc aagggccacg atgcctcccc
     4921 gaacaccccg cgccggatct ccatcacgtt tgaggacggc gtgcccgtcg ccctggacga
     4981 cgagcggatg ggtggcctgg cgctcgtgca gcgcgtgaat gccatcgcgg gcgagtacgg
     5041 cgtcggccgc atcgatatca tggaagaccg catgctcggg ctgaaggtgc gcgagaacta
     5101 cgagtgcccg gcggccgtga cccttctcac ggcgcaccgc gccctcgaag ccctggtgct
     5161 cacgcgtccg gagctcgata tgaaggcgat cgtcgaccgc aagtggagcg agctcgccta
     5221 tcagggcctg tggttcgacc cgctgaagga agacctcgac gcgttcatcg ccgccaccca
     5281 gcgccgcgtc accggcaccg tcttcctcga gctgttcaag ggacacgcga aggtgatcgg
     5341 gcgccgcagc ccctgggcgc tctatagcga ggacctggcg tcgttcgata gcaagagctt
     5401 tgaccagcgc gaggcggttg gcatggtcaa gaatcacggc atgcagagcc ggatgtacct
     5461 gcaactcaag tcgaagcttg cctgagtggc gataagcgat acggggtgca aaatcgacgg
     5521 cgggcaggag cctctgcccg ccgtctgctc gcatcccgcg ttgcacctcc ccatccccga
     5581 aaccgaagat gagaacactg tggagccatg gcgaggagcg gccggcggag gtgctggccg
     5641 aggagtgggg cgcgtcgttc gcgtttgacg cgcggctgct tccctacgat gtgcgcggct
     5701 cgatcgcgca cgcgcgcatg ctgggcgcaa ccgggatcat caccccggag gagtctgccc
     5761 gcattgtcgc cggcctggag gagatcctgc gcgagtggga agccgacccg acggtgcccg
     5821 tcaccgagta cgaggatgtg catacctacg tcgaggcgcg gctgaccgag aagattggcg
     5881 agaccgcgcg caagctgcac acggcgcgct cgcgaaatga ccaggtggcg ctcgacgccc
     5941 gcctcttcgt caaagacgcc gccggtgagg tgcgcgaggg cattcgccac ttccagcggg
     6001 tgcttgtcga gcgcgccgaa gagctggcgg acgtcatcct tcccggctac actcacctgc
     6061 agc
//
LOCUS       DAOSCV010000134         6063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8275_length_6063_cov_3.340546, whole genome shotgun sequence.
ACCESSION   DAOSCV010000134 DAOSCV010000000
VERSION     DAOSCV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8275_length_6063_cov_3.340546"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLY56_04700"
     CDS             <1..990
                     /locus_tag="PLY56_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20812.1"
                     /translation="RAIQQVPGARVSALCDSIQKRLDGDWRDIRGNIGESAGIVDLSG
                     INKYLEVDKFLADPEVDIVDVCLPTPFHRDIAIRAMEAGKHVLVEKPIALTLEDADAM
                     VEASRRTGKKLMVGQVVRFFPQFDFVKQAVASGEYGALLGIHLKRVISKPSWSAGGWF
                     NDPKQTGGIVLDLHIHDSDFIRYLLGMPDAVQCSGVVSPAGEVVYVSAQYLYEGRNLT
                     VTANSGAIASPTVVFEQGYDVYLEKATIRYNSLDEAPVTLYTDEGKQVVDLQKAVDLP
                     NPDGWVNEIAYAVKCLSEGKEPELLAGASARDSMYLCLKELESVSTGKPVKVS"
     gene            1166..1774
                     /locus_tag="PLY56_04705"
     CDS             1166..1774
                     /locus_tag="PLY56_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOJ20813.1"
                     /translation="MEQALKRLQEEIRACRACVERGYPATPPPVVGLGTRARILLVGQ
                     APGGMESDSRLPFSGPAGKRLFTWLERAGFDPERIREEIYFSALTRCYPGKNARGSGD
                     RRPSREERALCTPFLAREFALIDPKLILLVGQMAIEHFLGKMPLTQAVGAEFEEEDRF
                     YIPLPHPSGASTWLNSPENRDRLEHAIRLIRWHRTHLIPSKR"
     gene            2050..2727
                     /locus_tag="PLY56_04710"
     CDS             2050..2727
                     /locus_tag="PLY56_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20814.1"
                     /translation="MPKQSNRGFTLIELLVVIAIIAILAAILFPVFARARENARKSNC
                     QSNCKQLGMALMQYIQDYDETLPILGWLPGDGVVWPNGKWDACHPWHMRIYPYVKNTG
                     VFNCPSATYKWQGEVSQMIKYGANSTLIAVTPPCTLASIQRPADTVAIADSDGEASYG
                     IQQTPYLGSGSSKPRYLGVRHSDGANFVFMDGHVKWVKVQVDPVTKLPVHPGPQQGVY
                     WRADGTS"
     gene            complement(2986..4023)
                     /gene="aroF"
                     /locus_tag="PLY56_04715"
     CDS             complement(2986..4023)
                     /gene="aroF"
                     /locus_tag="PLY56_04715"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ20815.1"
                     /translation="MVIIMKTGASPEEIAEVTHSIEREGFTPFENPGVERKVIAVLGV
                     IHSHKLQLVDKFQNLPGVERVALISEPYKLPSRQCHPQDTVFEVGGVPVGGKTLAIFA
                     GPCSIESEAQAIETARAVKAAGANFFRGGAYKPRSSPYAFQGLGEEGLKILAACREET
                     GLPVITEVVDPDDVEKVCQYADFLQIGARNMQNYRLLQRVGQARTPVLLKRGFGTRLR
                     EMLMAAEYIMVEGNHRVALCERGIITFEDSTRYTTDINAVPVLKHLSHLPVILDPSHA
                     TGFSRYVGAVAKAGVAAGADGLLLEVHPNPAEALSDGPQSLKPEAFASLMRELARVAS
                     AVDRALPEQGA"
     gene            4420..4890
                     /locus_tag="PLY56_04720"
     CDS             4420..4890
                     /locus_tag="PLY56_04720"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20816.1"
                     /translation="MARTSGEPRAGETPEVGVVLVDSDTRELRVSSEFRHGHRVRAFV
                     REAVEECGFDKQAVADLMLAVTEIFNNAVEHAHHFEAHREVVIRVHRHQGAIQVDITD
                     QGPGFVPREAATPPASFLPTQRGLGLFLARNLMDEIHFSHGAGTTVHLVKHTPR"
     gene            complement(4914..5672)
                     /locus_tag="PLY56_04725"
     CDS             complement(4914..5672)
                     /locus_tag="PLY56_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ20817.1"
                     /translation="MSAGSIPAVVLAGGKPGEALATASGVRYKALVPVRGRPMVAYVL
                     EALAACERVSAVHVVAPEEVAAALPGVRHVADTGSFLGNLTAGVAACEGARFSLVITC
                     DIPFVTAEALAAFIALAEARDVDLAYPIVELGLCNERFPGMRRTSLRLREGRFTGGNA
                     VLIRPAFLQRNAGLIEEAFAARKQPLRLARMVGWDLVPRILLASKLPGLLPLSLAEER
                     VQRLVGGRVAAIPLAYPEIGADIDKPEDLGPLVA"
     gene            complement(5669..>6063)
                     /locus_tag="PLY56_04730"
     CDS             complement(5669..>6063)
                     /locus_tag="PLY56_04730"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="catalyzes the phosphorylation of
                     4-diphosphocytidyl-2-C-methyl-D-erythritol in the
                     nonmevalonate pathway of isoprenoid biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ20818.1"
                     /translation="LDAHLVIAKPAVGVSTARAYAELDALRAGRVPEPCDDAIATMRA
                     ALERGDRAGVVRALRNDLEAPVLAMHPEIAGLKAELLARGAEGALLSGSGSAVFGLFA
                     TEDAARECAGALAERWPWVCVARPWRSR"
BASE COUNT         1080 a         2118 c         1852 g         1013 t
ORIGIN      
        1 cgcgcgatcc agcaggtgcc cggcgcgcgc gtctccgcct tgtgtgactc gattcagaag
       61 cgccttgatg gcgattggcg ggacatccgc ggcaacatcg gggaatccgc cggtatcgtt
      121 gacctcagcg gcatcaacaa atacctcgaa gtagataagt tcctggccga ccccgaggtt
      181 gacatcgtgg atgtctgtct ccccacgcct ttccatcgcg acatcgcgat ccgcgccatg
      241 gaggcgggaa agcacgtcct cgtcgagaag ccgattgccc tcacgctgga ggatgccgac
      301 gccatggtcg aagcctcgcg gcgcacgggc aagaagctga tggtcggcca ggtcgtccgc
      361 ttcttccccc agttcgactt cgtgaagcag gcggttgcca gcggcgagta tggcgccctc
      421 ctgggaatcc acctgaagcg cgtcatctcc aagccgtcgt ggtcggctgg cggttggttc
      481 aacgacccga agcagacggg aggcattgtc ctcgacctgc acatccatga ctccgacttt
      541 atccgctacc tcctcggcat gccggatgcc gtgcagtgca gcggtgtcgt cagcccggcg
      601 ggcgaggtgg tctacgtctc cgcgcagtac ctctacgagg ggcgcaacct gacggtgacg
      661 gccaacagcg gcgccatcgc ctcgccgacg gtagtcttcg agcagggcta cgacgtctac
      721 ctcgaaaaag cgaccatccg ctacaactcg ctggatgagg cgccggtcac cctctacacc
      781 gatgagggca agcaggtggt cgacctgcaa aaggccgtcg acctgccgaa ccccgacggc
      841 tgggtcaacg agatcgccta cgcggtgaag tgcctcagcg agggcaagga gccggagctc
      901 ctcgccggag cctcggcgcg tgactcgatg tacctctgcc tgaaggagct cgagtccgtt
      961 tccaccggca agccggtcaa ggtcagctaa ccttcctggc cgcgtggggg cagatctccc
     1021 cgcccgggct ttgcccccgc gcggccgtcg tttcacttcc ccttttccac tctcgccctg
     1081 cgtgacccca cgcatagggc tctcccgatg atattcctct tgatgcaggc tgaaccgatc
     1141 ccgacgcttt gctgtccttg ggagtatgga acaggcactc aagcggctac aagaggagat
     1201 ccgggcgtgc cgggcgtgcg tcgagcgggg ctacccggcc accccaccgc cggtcgtcgg
     1261 tctgggcacg cgcgcccgca tcctgctggt gggccaggcg cccggtggga tggagagcga
     1321 cagccggctt ccgttcagtg gtccggccgg caaacgcctc ttcacgtggc tggagcgcgc
     1381 cggcttcgat ccggaacgga tccgcgagga gatctacttc tcggcactga cgcgctgtta
     1441 ccctggcaag aacgcgcgtg gatccggcga ccggcggcct tcgcgcgagg aaagggcgct
     1501 ctgcacgcct ttcctcgccc gagagttcgc cctgatcgat ccgaagctga tactcctcgt
     1561 gggtcagatg gcgatcgagc acttccttgg caagatgccg ctcacgcaag ccgtgggcgc
     1621 ggagttcgaa gaggaggacc gcttctacat cccactcccc cacccctcgg gcgccagcac
     1681 ctggctcaac tctcccgaaa accgcgaccg cctggagcac gcaatccgct tgatccgctg
     1741 gcaccgcacc cacctgatcc catccaagag gtgatgcccc cattcccgca gttcggaagc
     1801 cgctaggtgg aatgccggac ccttacggag tccgtagaaa gccacgcggt agctctcctt
     1861 ccccagggcc ggcatgaggc cggaggatag gaaaccacac gtctgtcccc ttcatccgcg
     1921 gtactgatgc cggcgcaggg ccgatggcac gcccatttgg gctcgcttga cagccaggcg
     1981 tgcgcgtgct acgataatcc ccggttacta caggcggcgc tcgctgcgcc ggtgtggaag
     2041 gaaagccgta tgccgaaaca gtccaatcgc gggtttaccc tcatcgagtt gctcgtagta
     2101 attgcgatca tcgctatact tgcagcgatt cttttccctg tgttcgcacg ggcgcgcgag
     2161 aacgcccgca agtcgaactg ccagtccaac tgcaagcagc tgggaatggc gctcatgcag
     2221 tatatccagg actacgatga gactctgccg attctcggct ggctcccggg tgacggcgtc
     2281 gtctggccga acggcaagtg ggatgcctgc cacccctggc acatgcggat ctacccgtac
     2341 gtgaagaaca ccggcgtctt caactgcccg agcgccacct acaagtggca gggtgaggtc
     2401 tcccagatga tcaagtatgg ggcgaactcc actctgatag ccgtgacgcc gccgtgcacc
     2461 ctggccagca ttcagcgtcc ggcggatacc gtggccatcg ccgattcgga cggcgaagcc
     2521 agctatggca ttcagcagac cccttacctc ggcagtggct ccagcaagcc gcgctatctg
     2581 ggcgtgcgcc attcggatgg cgccaacttc gtcttcatgg atggccacgt gaagtgggtc
     2641 aaggtgcagg tggacccggt caccaagctt ccggtgcatc cgggtcctca gcagggcgtc
     2701 tactggcgcg ccgacggcac gagctgagaa tcgacgctcc ttctctaaag ggggccgggc
     2761 ttgcgtggca agcccggccc cctttgttct gggattccga gataaggaat ccgaagccac
     2821 ggaggcagcg cgcaacccgg cgcgcagcct tctccttact tcccggcggc tccgcatcgc
     2881 ggtcaaagag catgaacgtc gcgcgccgcc gtttatcgcc acaatcccct cacccgcgcc
     2941 cgtcgtcatc gcggaaatgg gaagacccgt catccggtcg ggccgtcacg ccccctgttc
     3001 cggaagcgcg cggtcgaccg cactggccac gcgcgccagc tcgcgcatca ggctggcaaa
     3061 cgcctcgggc ttcagcgact ggggcccatc cgagagcgcc tcggcaggat tgggatgcac
     3121 ctcgaggagg aggccatcgg cgcctgcagc cacccctgcc ttcgcaaccg cgccgacata
     3181 ccgggagaaa ccggtggcat ggctcgggtc caggatcacc gggaggtggc tgaggtgctt
     3241 gagcaccggc accgcgttga tatcggtcgt ataacgggtg gagtcctcaa aagtgatgat
     3301 cccgcgttcg cagagcgcca cacggtggtt cccctccacc atgatatact cggcggccat
     3361 cagcatctcg cgaagccgcg tcccgaagcc tcgcttcagc aagacgggcg tgcgcgcctg
     3421 ccccacccgc tgcaacagcc ggtagttctg catattgcgc gcgccgatct ggaggaaatc
     3481 ggcatactgg caaacctttt ccacatcgtc cggatcgacc acctccgtga tcaccggcag
     3541 gcccgtctcc tcgcggcacg cggcgaggat ctttaggcct tcttcgccca ggccctggaa
     3601 agcgtagggc gatgagcgcg gcttgtaggc gcctcctcgg aagaagttgg cccccgccgc
     3661 cttgaccgcg cgcgccgtct ctatcgcctg cgcctcgctc tcgatgctac aagggccggc
     3721 aaagatggcg agcgtcttcc cacccaccgg cacaccaccc acttcaaaca ccgtgtcttg
     3781 agggtggcat tgccgtgagg gcagcttgta tggctccgag atgagcgcca cgcgctcgac
     3841 gccgggcaga ttctggaact tgtccaccaa ctgcagcttg tgcgagtgga tgacgccgag
     3901 cacggcgatc actttacgtt cgacccccgg gttctcgaaa ggcgtgaagc cctcccgctc
     3961 aatcgagtgg gtcacctccg caatttcctc ggggcttgcc cccgtcttca tgatgataac
     4021 catagagagc aatctcctac aagcgcggcc ccgctgaggg ccaccgcaat acgcttccgg
     4081 tgagatagta tcaccggttc aacaccctca ggagtgccac cggggcgtgg tccgttcctc
     4141 gagggatgct ttcatgccgg gaacaaacaa ggaaaaccgt gccactcgct ctccgccaac
     4201 gccctctccc gaccggcgag gcagatcccg ctcgccgcac gctccgtccg atggaacacg
     4261 ccccccagcc tgtgcctcag gcgccggcac gcccacgagg agggagttga cgaggcgttc
     4321 tggctatgat acaatcccag cgttcggcct tccagtgcca ggccatcggg cccggcgagg
     4381 atcgcaccca acgagcgccg ccgccgtcag gtcgcccgct tggcccggac atcgggcgag
     4441 ccgcgtgccg gcgaaacgcc cgaggtaggc gtggtgttag tagatagcga tactcgcgag
     4501 ttgcgggtga gcagcgaatt ccgccatggc caccgcgtgc gtgccttcgt gcgcgaggcg
     4561 gtcgaagagt gtggcttcga caagcaagcc gttgcggatc tgatgcttgc cgtcacggag
     4621 atcttcaaca acgcggttga acacgcacac cacttcgagg cccatcgcga ggtcgtcatc
     4681 cgggtgcatc gccaccaggg tgccatccag gtggatatca cggatcaggg cccaggcttc
     4741 gtaccccgtg aagcagcaac cccgccggcc tcattcctcc ccacgcaacg tggtctcggg
     4801 ctattcctgg cccgcaatct aatggacgag atccacttca gtcacggcgc cggcaccacc
     4861 gtccacctgg tgaagcacac cccccgctag cgagcatcca cggcgaacgc gccctacgcc
     4921 accaacggcc ccaggtcttc tggcttatca atgtcggctc caatctccgg gtacgcgagc
     4981 gggatcgccg cgactcgccc tccgaccagg cgttggacgc gctcctcggc caggctcagc
     5041 ggcaacaacc caggcagctt gctcgccaac agaattcgcg gcacaaggtc ccagccgacc
     5101 atgcgcgcga gcctcagagg ctgcttccgg gcagcgaacg cttcctcgat gaggcccgcg
     5161 ttgcgctgca ggaacgccgg gcggatcagc acggcattcc cgccggtgaa gcgtccctca
     5221 cgaaggcgaa ggctggtccg gcgcatcccc gggaagcgct cgttgcacag cccgagttcc
     5281 acgatcgggt aggccagatc gacatcgcgc gcctcggcga gcgcgatgaa ggccgcgagc
     5341 gcctcggccg tgacgaacgg aatgtcacac gtgatcacca gcgagaagcg cgctccctcg
     5401 catgccgcca cgcccgcggt gaggttcccg aggaagctgc ctgtatctgc cacgtgccgc
     5461 actcccggca gggctgccgc gacctcctcg ggagcaacca cgtgaaccgc cgagacacgc
     5521 tcgcaggcgg ccagggcctc gagcacatag gcgaccattg gcctcccacg cacgggcacc
     5581 agcgccttgt agcggacgcc ggatgcagtg gcgagcgcct ctcccggctt tcccccagcc
     5641 agaaccaccg ccggtatcga ccctgcgctc atcggcttct ccacgggcga gcgacacaca
     5701 cccacggcca tcgctccgcc agcgcgccgg cgcactcgcg cgccgcatcc tccgtcgcga
     5761 aaaggccaaa cacggccgat ccgctgccgg aaagcagcgc cccctccgcg cctcgcgcca
     5821 ggagttctgc tttcagcccg gcaatctccg ggtgcatggc caggaccggt gcttccaggt
     5881 cattgcgcag ggctcggaca acgcccgcgc ggtcgccacg ctccagcgcg gcacgcatcg
     5941 tcgcaatcgc gtcatcgcac ggctcgggaa ctcgcccggc gcgcagggca tccagctccg
     6001 cgtaggcgcg cgcggtcgaa acgcccaccg ccggcttggc gatcacaagg tgtgcatcga
     6061 gcg
//
LOCUS       DAOSCV010000135         6052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8288_length_6052_cov_3.385193, whole genome shotgun sequence.
ACCESSION   DAOSCV010000135 DAOSCV010000000
VERSION     DAOSCV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_6052_cov_3.385193"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLY56_04735"
     CDS             <1..385
                     /locus_tag="PLY56_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ20819.1"
                     /translation="VEKLVTAVNHPNFGVLIDIGNFICVDEDPRVAVGRLIPYAFHCH
                     AKDFHLKPGTLPYPGKPWGVTRGGNYYRGAIIGHGDVPLLPCLRVMKRAGYSGVLSIE
                     FEGMEDVLTALELGQANLRQLVEMA"
     gene            488..2122
                     /locus_tag="PLY56_04740"
     CDS             488..2122
                     /locus_tag="PLY56_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20820.1"
                     /translation="MASVKQRILQVMAEGVTDRLPQFDPATGRFLQEPDGPPAPGARP
                     DEIGWCVINQDIIYPLAVLYTTPGTSFYGDRRILEMVQRGGDAIRDFQYPDGQVEFLK
                     IDGSRWGPTYMAWTNYAWLETYALLRDELGEERRKRWEEGLTLAHDGQAREISNLHVH
                     NIPCWKGMSCYRAGQLFGRPDWQDAGTAMIQAVVAAQQPGGYWAEHGGPSTLYNYVYV
                     HGLGLYYRFTGDERVLPALEAATDFHQAFLYPGGALVETVDGRVSYHDRIMLMGWVGF
                     SACPQGRRLVRYLAEHLNAKRDLHSYQGGAIASSAQHLIEGEEAPIWLDRPSFHEAYR
                     DWALVCREGPWFACLSAFTCPPVASRWGQDRQAFLSLHHDERGLILGGGNSKDQAEWS
                     SFVASGRLMPDRGELLPEGDGIALTYGAVRCLLRIRMDGTRVILTGEAEGGPALQQFI
                     VQARPGDTVTSATGRVATLEAGTALQWGPAELGAWIEVSGARIAVPQGAAFRWPTAAF
                     NPYAADGAAPFGAERGVLSARIDGRPIQWEITAQSR"
     gene            2136..2963
                     /locus_tag="PLY56_04745"
     CDS             2136..2963
                     /locus_tag="PLY56_04745"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ20821.1"
                     /translation="MKKRLALVGAHPDDCDILGSGLAMRYRELDWDVMFVSMANGDCG
                     HHEMPRQELAARRKRETEAVADMLGIEYVVLDTSDCEVFPTLEQRWTLLRVLRRFEPD
                     LVVTHHATDYHPDHRYTSQLVGDVSYLLKVPHCCPDVPALRKDTVHAFFHAWRDTARP
                     DSFCVAIDIDDLWQRKVEVIAKHESQMFEWLPWVDGQDMSQIPPPEDAEGRLRYVDER
                     RGPRAERIADLYRDRLCELYGPTRGRQIRKAEAYVTAPFPTSLSEENYRELFPFFGG"
     gene            2965..3972
                     /locus_tag="PLY56_04750"
     CDS             2965..3972
                     /locus_tag="PLY56_04750"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20822.1"
                     /translation="MKEKTLRVGLIGCGMITQRSHAPGFAKIPDLQIAALCDPVPDRM
                     VAVRDAHAPDAALFESYEELLASGLVDAVTVATPVYLHGPVTIAALKAGCHVLCEKPM
                     AMSRAEAVEMVDAARTAGKVLQINLSRRYDPFYQMIAQLIAEGRIGEPRHIRASRVHT
                     TAPDQGWSPGARWFVTKAQGGGIVGDIGVHVGDMMQWYMGPVESVSAHTATIRPDIDV
                     VDNATALFRFRGGATGVLELSWTSPVNRMCFEIQGTEGLLLATTQNEPVRILRRDGTT
                     EEYGPEQFPQGVRNSFECFADAIRGIAPSPAPGEIGLSIQAVLDAILESGAEGGAPVR
                     V"
     gene            complement(4046..5245)
                     /locus_tag="PLY56_04755"
     CDS             complement(4046..5245)
                     /locus_tag="PLY56_04755"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOJ20823.1"
                     /translation="MMLLENPRALRDQYAKALLEDVIPFWLRHSPDRECGGYFTCLER
                     DGRVFDTDKFVWLQARQVWMFSKFYNALEPRPEFLEIAALGARFLADHGHDANGDFYF
                     ALDRRGTPLVQPYNIFSDCFAAMAFAEFGRASGEEWATQLATRTFERILARWENPKGQ
                     YNKLVPGSRPMRSMVLPMITINLALELGGALPDYPVADVIRENLARVMDDFVDPDTHL
                     VYEHIAPDGSHPDTFDGRLINPGHGIEAMWFVMDAAHQLGEHTLVERAADALEAQLQF
                     GWDPEYEGIFYFMDALGKPPQQLEWDQKLWWVHMETLVGLLLGWHLTRRQSLAEWYDR
                     VHAYAWSHFPDPEYGEWWGYLNRRGEVLLNLKGGKWKGCFHVPRGLFRCWRILDEILA
                     SQEAGNA"
     gene            complement(5287..>6052)
                     /locus_tag="PLY56_04760"
     CDS             complement(5287..>6052)
                     /locus_tag="PLY56_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20824.1"
                     /translation="HPGVDAAGATSWFIGVGDPSEQIATLRRAGIHRACGAPIQVMQP
                     DSFAPIRRLNDQALALRDRFPEFYIPGIQVHPRFPEESCAEIERCCGGEGVRWIGELV
                     GYLMGFGEEYASRNAVKIFREAARFGAVINFHCASLDVIERACRAVPEAQFVLAHPGG
                     GREEFLARIALVARLSNLHLDISGSGIDRYGILHKAIDVAGKEKLLFGTDYPINNPAV
                     YVQGALFERLSEEEYAALFHGNFRRLTGIAGEGSGG"
BASE COUNT          995 a         1970 c         2096 g          991 t
ORIGIN      
        1 cgtggagaag ctggtcaccg cggtcaacca cccgaacttc ggcgtgctga tcgacatcgg
       61 caacttcatc tgcgtcgatg aggacccgcg tgtcgccgtg ggccggttga tcccctacgc
      121 gttccactgc cacgccaagg atttccacct gaaaccgggc acgctcccct acccgggcaa
      181 gccgtggggc gtgacgcgag gtggtaacta ctaccgcggc gcgatcatcg gtcacggcga
      241 cgtgccgctg ctcccctgcc tccgcgtgat gaagcgagct ggctattctg gcgtcctctc
      301 catcgagttc gaggggatgg aggatgttct gaccgcactc gagcttggcc aggcgaacct
      361 ccggcagctg gtcgagatgg cgtgacgatt tgccgggcgc tggctgcttt ccgggaagag
      421 cggcgcccgg cttcccgcct ggcatgtgcc cggcgggacc gagagatccc ggtagagaga
      481 gccagagatg gccagcgtga agcagcgaat cctccaggtg atggcggagg gcgtcaccga
      541 caggttacct cagttcgatc cggccaccgg ccggtttctc caggagccgg atggcccgcc
      601 ggcgcccggc gcccggcctg acgagatcgg ctggtgcgtc atcaaccagg atatcatcta
      661 tcccctcgcg gtgctctaca ccacccccgg cacctcgttc tatggcgacc ggcggatcct
      721 ggagatggtt cagcgcggcg gagacgcgat tcgcgacttc caataccccg acgggcaggt
      781 cgagttcctg aaaatagatg gcagccgctg gggcccaacg tacatggcct ggaccaatta
      841 cgcctggcta gagacctacg cgctcctgcg cgacgagctg ggcgaggagc ggcggaagcg
      901 ttgggaagaa gggctcactc tcgcccatga cggccaggcg cgcgagatca gtaaccttca
      961 tgtccacaac atcccgtgct ggaaggggat gagctgctac cgcgccggtc agctcttcgg
     1021 gcgcccggat tggcaggacg cgggcacggc gatgatccag gctgtcgttg ccgcgcagca
     1081 gccgggcggg tattgggcgg agcacggcgg ccccagcacg ctctacaact acgtctacgt
     1141 gcacggcctg gggctctact accggttcac gggcgacgag cgcgtcctgc ctgccctgga
     1201 ggccgccacc gatttccacc aggcgtttct ctacccgggt ggcgcgctcg tggagacggt
     1261 ggacgggcgc gtcagctacc atgatcgcat catgctcatg ggctgggtgg gcttctccgc
     1321 ctgcccgcaa ggccggcgcc tggtgcgcta tctggcggag catctgaacg cgaagcgcga
     1381 cctgcacagc tatcaggggg gagcgattgc cagcagtgcg cagcacctga tcgaagggga
     1441 ggaagcgccc atctggctcg accggccatc gttccacgag gcgtatcgcg attgggcgct
     1501 tgtttgccgc gagggcccgt ggtttgcctg tctcagcgcc ttcacctgcc cgcccgtggc
     1561 cagccgctgg gggcaggatc gccaggcgtt cctgtcgctg catcacgatg aaaggggcct
     1621 gatcctcggc ggtggcaact ccaaagacca ggccgaatgg tcgagcttcg tagcgagtgg
     1681 gcggctgatg cccgatcggg gcgagctttt gcccgagggc gacgggatcg cgctcaccta
     1741 cggcgcggtt cgctgtctct tgcgaatccg gatggatggc acgcgagtga tcctgacggg
     1801 agaggccgag ggcgggcctg cgctgcagca gttcatcgtg caggcgcgac cgggcgacac
     1861 cgtgacctcg gccacggggc gcgtggcgac cctggaggct ggcacggcgc tccagtgggg
     1921 cccggcggag ctgggcgcgt ggatcgaggt gagcggcgcg cggatcgcgg tgccgcaggg
     1981 cgccgcattc cgctggccga ccgccgcgtt caacccctac gcggccgatg gcgccgcgcc
     2041 cttcggagcg gaacgcggcg ttctctccgc gcgtattgat ggccggccca tccagtggga
     2101 gatcacggcg cagagccggt gaggaggagg tcaaggtgaa gaaacggttg gcattggtcg
     2161 gcgcgcaccc ggatgactgt gatatcctcg gcagcggcct ggccatgaga taccgcgagc
     2221 tggattggga cgtgatgttc gtctccatgg cgaacggcga ttgcggccac cacgagatgc
     2281 ctcgccagga gctggcagcg cggcgcaagc gtgagaccga ggccgtcgcc gacatgctcg
     2341 gcatcgaata cgtggtgctg gatacctccg attgcgaggt cttcccgacg ctggagcagc
     2401 gctggacctt gctccgggtg ttgcgccgct tcgagccgga cctggtggtg acgcaccacg
     2461 ccaccgatta ccacccggac catcgctaca cgagccagct ggtgggcgac gtctcctact
     2521 tgctgaaagt gccccactgc tgcccggatg tgcccgcgct acgcaaggat accgtccacg
     2581 ccttcttcca cgcctggcgc gacacggcgc ggccggacag tttctgcgtg gcgatcgata
     2641 tcgatgacct ctggcagcgc aaggtggagg tgatcgcgaa gcacgagtcg cagatgttcg
     2701 agtggctgcc gtgggtggat ggccaggaca tgagccagat cccgccgccc gaggacgcgg
     2761 aggggcggct gcgatacgtc gatgagcggc gcggtccgcg cgcggaacgg atcgccgatc
     2821 tctaccgcga ccggctgtgc gagctgtatg gcccaacgcg cggcaggcag atccgcaagg
     2881 cggaagccta cgtcaccgcg ccattcccca cctcgctcag cgaggagaac taccgggagc
     2941 tcttcccgtt cttcgggggg taagatgaaa gagaaaacgc ttcgagtcgg cttgatcggc
     3001 tgcggaatga tcacccagcg cagccacgct ccgggctttg caaagattcc ggatctccag
     3061 attgcggcgc tgtgcgaccc ggttccggac aggatggtcg cggtgcgcga cgcgcacgcg
     3121 ccggacgccg ccctcttcga gagctacgag gagctgctcg cgtccgggct ggtcgacgcg
     3181 gtcaccgtgg ccacgccggt ctacctgcac gggccggtga cgatcgcggc gctcaaggcg
     3241 gggtgccacg tcctgtgcga gaagccgatg gcgatgtcgc gtgccgaggc ggtcgagatg
     3301 gtggatgccg cgcgcacggc ggggaaggtc ctccagatca acctctcgcg ccgctacgat
     3361 ccgttctacc agatgatcgc gcagctgatt gctgagggcc ggatcggcga gccgcggcac
     3421 attcgcgcga gccgcgtgca tacgaccgcg cccgatcagg ggtggtcgcc cggtgcccga
     3481 tggttcgtca ccaaggcgca gggcggcggt atcgtgggcg atatcggcgt ccacgtgggc
     3541 gatatgatgc agtggtacat ggggccggtc gaaagcgtga gcgcacacac ggcgacgatt
     3601 cgccccgata tcgacgtggt cgacaacgcc acggcactct tccgcttccg tggcggcgcc
     3661 accggcgtgc tggagctaag ctggaccagc ccggtcaacc ggatgtgctt cgagatccag
     3721 ggcaccgagg ggctgcttct ggccaccaca cagaacgagc cggtgcggat cttgcggcgt
     3781 gacggcacca ccgaggagta cgggcccgag cagttcccgc aaggggtgcg caacagcttc
     3841 gagtgcttcg cggacgcgat tcgcggcatc gcgccctcgc ccgctcccgg agagatcggc
     3901 ctctccatcc aggccgtgct cgacgcgatc ctcgagtccg gcgccgaggg cggcgccccg
     3961 gtgcgcgtgt agagactctg gaagccggtc cggcgctttc ggccgggccg gctttcttta
     4021 ctccgggcgg ggttgccccc acgcgtcagg cgttgccggc ctcttgcgag gccaggatct
     4081 cgtccaggat ccgccagcaa cggaagagcc cgcgcggcac gtggaagcag cctttccact
     4141 tgccaccctt caggttgagg agcacctcgc cgcgccggtt gagatagccc caccactcgc
     4201 catactccgg gtcggggaag tgtgaccagg cgtaggcgtg cacgcggtca taccactcgg
     4261 ccaggctctg gcgccgggtg aggtgccagc cgaggagcaa gccgacgagc gtctccatgt
     4321 gtacccacca cagcttctgg tcccactcga gctgctgggg cggcttgcca agcgcgtcca
     4381 tgaagtagaa gatcccctcg tactccggat cccagccgaa ctggagctgc gcctccaggg
     4441 catcggcggc gcgctcgacg agggtgtgct cgcccagctg gtgggcggca tccatcacga
     4501 accacatcgc ctcgatcccg tggcccgggt tgatcagccg gccatcgaag gtgtccgggt
     4561 ggctgccatc gggggcgata tgctcgtaga cgaggtgggt gtcggggtcg acgaagtcgt
     4621 ccatcacgcg cgctaggttc tcgcggatca cgtcggccac cggatagtcg gggagcgctc
     4681 cgccgagctc cagcgccagg ttgatcgtga tcatcggaag gaccatggag cgcatgggac
     4741 ggctgccggg caccagcttg ttgtactggc ctttcgggtt ctcccagcgc gcgaggattc
     4801 gctcgaaggt gcgcgtggcg agttgcgtcg cccactcctc gccgctggcg cgcccgaact
     4861 cggcgaacgc catggcggca aagcagtcgg agaagatgtt gtagggctgg acgagtggcg
     4921 tgccgcgacg gtcgagcgcg aagtagaagt cgccgttggc gtcatgaccg tggtcggcga
     4981 gaaagcgcgc gccgagcgcc gcgatctcca ggaactcggg ccgcggctcc agggcgttgt
     5041 agaacttcga gaacatccac acctggcgcg cctgcagcca gacaaacttg tcggtatcga
     5101 aaacgcggcc gtcgcgctcc aggcaggtga agtagccgcc gcactcgcgg tcgggcgaat
     5161 ggcgcagcca gaaggggatc acgtcttcca gcagcgcctt ggcatattga tcgcgcagcg
     5221 cgcgtgggtt ctccagcagc atcattcttt cctctccgat ccggggctgg cgtgctcctc
     5281 tcgggcttat ccgccgcttc cttcgcctgc tatgccggtc agccggcgaa agttgccgtg
     5341 gaacagcgca gcgtactcct cttcggacag gcgctcgaac agggcgccct gcacgtagac
     5401 cgcggggttg ttgatcgggt agtccgttcc gaagaggagc ttctccttgc cggccacgtc
     5461 gatcgctttg tggaggatgc cgtagcggtc gatgccggag ccgctgatat cgaggtgcaa
     5521 gttggagagg cgtgcgacca gcgcaatccg cgccaggaac tcctcgcgcc cgccacccgg
     5581 atgagcaagc acgaactgcg cttccggcac ggcgcgacag gcgcgctcga tcacgtccag
     5641 gctggcgcag tggaagttga tgacggcgcc aaaccgggcc gcctcgcgga agatcttcac
     5701 agcgttccgg gaggcgtact cctcgccgaa gcccatcagg tagcccacca gctcgccaat
     5761 ccaacgcacg ccctcgcccc cgcagcagcg ctcgatctcg gcgcacgatt cctccgggaa
     5821 gcgggggtga acctgaatcc ccgggatgta gaactccggg aaacggtcgc gcagcgccag
     5881 cgcctggtcg ttcagccggc ggatcggcgc gaaagagtcc ggctgcatca cctggatcgg
     5941 cgcgccgcac gcgcggtgaa tcccggcccg gcgcagcgtc gcgatctgct ccgagggatc
     6001 gcccacgccg atgaaccacg aagtggcacc cgcggcatca actccggggt gg
//
LOCUS       DAOSCV010000136         6046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8297_length_6046_cov_3.529127, whole genome shotgun sequence.
ACCESSION   DAOSCV010000136 DAOSCV010000000
VERSION     DAOSCV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_6046_cov_3.529127"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2666
                     /locus_tag="PLY56_04765"
     CDS             <1..2666
                     /locus_tag="PLY56_04765"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOJ20825.1"
                     /translation="CPRRDTPQPEEGGSLPHSAMEFLSQALPRTLDPEEFRNPGPAFR
                     PVMMWIWNANLDRVTLREQVAEMAARGIGGFFIHPMPDAFRKHDFIEGMTEPYLGEMF
                     FEWVKDAAEVAREHDLFCWLYDEGGWPSGTLVGRLADGRPELAGRVLRVQCLEAGDTG
                     APDAGSGTEEEVVTLPAPGGKRLRFYASREGYPTDLMNPATTEAFLAATHDAYARCLD
                     GEFGGLVPGVFTDEAAVGGVMGTDRVPWTPALPEEFRRRKGYDLLPYLPVLFGDAAIP
                     AEARPALSPEQAARVRYDFGDVWTRLHADAFYGRMSEWCREHGLLLVGHVGGEDDLIY
                     HATRGFGHFFRIMRYLDVPGVDAIWHQLFPGEPRSHFSKLAGSAARVHGKPLALSETF
                     AVYGYGLTYAQMKWVTDRQFGDGINVMVPMAAPYTTTRGRTYGTMSNLAWGNPNWPLF
                     KTYADYTARLSYLSRLGRPVARVALFYPASSLWVEADPAIAKSFHSVMDLLQEAQIDF
                     DIVDEEDLAGASVESGTLRIGVQAYQAVVLSDTRAFPWAVVERLAAFKEAGGAVAAVD
                     RLPWLCAEAEHQAEFKLALGALFSGERARFFPPEERVRLPEFLARLISRDFVLETPDS
                     NIRYSHRVWDETRLYFVVNASETARAITAVVAGTGSVDLWDPDRGTVTPVEGEPCGDR
                     TRVHLELPPLGSVFVVVRPGGQPRKPHSEPTPAGELPLKGSWEIAPVRVCQLVQGEAR
                     VDAPLPGCEAWSPTELRPWAELGLGTFSGSIAYRRVFSLSELEAARKAVLDLGTVKYA
                     AEVWVNGRAVGARLWPPFTLDVTGYLRPGRNEIRVVVTNTWANQFLQPEEMAHARERG
                     WYNTYAQRVEPFQRESLESGLLGPVSLRLFE"
     gene            complement(2772..5054)
                     /locus_tag="PLY56_04770"
     CDS             complement(2772..5054)
                     /locus_tag="PLY56_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20826.1"
                     /translation="MAVKDQSGAVRPVDSGLTGRVKEFALSQLGADLVGIANIERFEN
                     APLRMSPQGILPSARSVVVMALHHPDACIEMGGIEHPQVIGPYRVQYWMNTRLDEISY
                     RMGLFLEKEGYAAVPIVSSNIWRYKGYKDLTAHFAPDISHIYASVAAGLADLGYSGLA
                     ITPEYGARQRYVTVITDAPLQPTPLLEPGSVCDMCKLCVKHCLSKALSKEVDGLDELW
                     IEGKKYTKARKNLWRCAWGEHFDLDLDLPIPDHVNEEVILEQVRIHGFRGGEMGSCLR
                     YCLPKERRYFDSSYTNAPRRKRNTPDELASRRGLEEQVRSLAYRWAADFVMVTPQADL
                     EAIGIRPTEYLPDGQTAVTVGLHFRTPEGADPAAPARQYLLEMAAYDIARALERQGYS
                     AVCDTAFPEKSFQTTFTGVKEGWSVQTATVITAAPLEPTSRELPQVSVPAPTPEETRA
                     QLKRLLHEWGADLASVVPAERLAALHPQLAALFDGVEVLVARDRSPRMREYDPEVHTE
                     VTRTRAPEDHLKGARSVIVVGLRLPRASVERTALPPAEAVGPYAFAQYESVKLLRNIG
                     YRAIRWLEDRGYRATMSFDLCGTGSVVANPRGEQPDAFCNRFTAVAAGLGHLGKGGFV
                     ITPEFGPNVRFVAIVTDAPIAADPIPAEYLQPVDCGDCRRCLDACHTCAFQDEATVEV
                     NGVAERFYRMDRNRCDWAKRYSLVGEEGVKYVGWEMNVPLPEKIDAEALAEGLRQQPP
                     IPKHRPCNFETCVLACPYSR"
     gene            complement(5196..5954)
                     /locus_tag="PLY56_04775"
     CDS             complement(5196..5954)
                     /locus_tag="PLY56_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOJ20827.1"
                     /translation="MAATVPIQSIKRALDVLDALMEQGLGGEGVSLQAIAGRLGVPPS
                     TAHNILKTMVMCGYVARDDQHLYRLGPRCRDLARGSTLSAKFMAAAQGTIHSLAERTG
                     ESVVFATLVHGRRYPLLRADGHAVVRVSPTYEELSAFFRMVTGRVLAAYATPEELSEL
                     LAVHGLPGESWNGIETEETLEAALAEVRRAGMAEEECGRTQVYSLAVPVLDAREQLLG
                     ALGIHLPASRATPEHREKIRREIAAAANRLGQQA"
BASE COUNT          921 a         2036 c         2037 g         1052 t
ORIGIN      
        1 aatgtccccg acgcgacacc cctcagcctg aagagggcgg ttccctgccg cattctgcta
       61 tggagtttct gtcgcaagcc ctgccacgga cgctcgatcc ggaggagttt cggaacccgg
      121 gaccggcgtt ccgcccggtg atgatgtgga tctggaacgc caatctagac cgggtcaccc
      181 tccgcgagca ggtcgcggag atggccgcca gagggatcgg cggcttcttc atccacccga
      241 tgccggatgc cttccgcaag cacgacttca tcgaggggat gaccgagccc tacctgggcg
      301 agatgttctt cgagtgggta aaggacgcgg ccgaggtggc gcgcgagcac gacctcttct
      361 gctggctcta cgacgagggc ggctggccca gcggcaccct cgtcggtcgg ctggccgatg
      421 gccggcccga actggccggg cgcgtcctgc gcgtgcagtg cctcgaggct ggggacaccg
      481 gcgcgccgga cgcgggctct ggaacggagg aagaggtggt gacgctgccg gcgcctggtg
      541 gcaagcggct ccgcttctat gccagccgcg aggggtatcc gaccgatctg atgaacccgg
      601 caacgacgga agccttcctc gccgcgacgc atgacgccta cgcgcgctgc ctggacggcg
      661 aattcggggg cctcgtgccg ggagttttca ccgacgaggc ggcggttggc ggcgtgatgg
      721 gcaccgaccg tgtcccctgg acgcctgctc tcccggagga gttccggcgg cgcaagggct
      781 atgacctcct tccctacctc cccgtgctct tcggggacgc ggcgatccct gcggaagccc
      841 ggcccgcgct gtcgcccgaa caggccgcgc gcgtccgcta cgacttcggc gacgtctgga
      901 cgcggctcca cgccgatgcg ttctatgggc ggatgagcga gtggtgccgt gaacacggcc
      961 tcctcctggt gggccacgtg ggcggcgagg atgacctgat ttaccatgcc acgcgaggct
     1021 ttggccactt cttccgcatc atgcgctacc tcgatgtgcc gggcgtcgac gccatctggc
     1081 accagctctt ccctggcgag ccgcgctcgc acttctcgaa gctcgccgga tcggcggcgc
     1141 gcgtgcatgg caagcccctc gccctctccg agacgttcgc cgtctatggc tatggcctca
     1201 cctatgccca gatgaaatgg gtgaccgacc gccagttcgg cgacgggatc aacgtgatgg
     1261 tcccgatggc cgccccctac accacgacgc gcgggcgtac ctacggcacc atgtcgaacc
     1321 tcgcctgggg caaccccaac tggccgctct tcaagacgta tgccgactac accgcccggc
     1381 tgagctacct ctcgcgcctc ggccggccgg tggcacgcgt ggcgctcttc tatccggcga
     1441 gcagcctctg ggtggaggcc gatcccgcca tcgccaagag cttccactcc gtgatggacc
     1501 tcctccaaga ggcgcagatc gacttcgata tcgtcgatga ggaggacctg gcgggggcct
     1561 cggtagagag cggaaccctg cggatcggcg tgcaggcgta tcaggcggtg gtcctatccg
     1621 acacgcgcgc gtttccgtgg gcggtggtgg agcgcctggc cgcgttcaag gaggccggcg
     1681 gtgcggtcgc cgccgtggac cgcctcccct ggctctgcgc cgaggcagag catcaggcgg
     1741 agttcaagct agccctgggc gcgctcttct ctggagagcg ggcgcgcttc ttccctcccg
     1801 aggagcgcgt gcgtctgccc gaattcctcg ccaggctgat ctcgcgcgac ttcgtgctgg
     1861 agacgcccga ctccaacatt cgctattcgc accgggtgtg ggacgagacg cgcctctact
     1921 tcgtggtgaa cgcatcggaa accgcccgcg cgatcaccgc cgtggtcgcc ggcaccggtt
     1981 cagtggatct ttgggacccg gaccgcggga ccgtgacccc ggtcgaggga gaaccctgcg
     2041 gcgaccggac tcgcgtgcat ctcgagctgc caccgcttgg gagcgtcttc gttgtcgtgc
     2101 gtccgggtgg gcagccgcga aagccgcact ctgagcccac gccggccggc gagctccctc
     2161 tgaagggatc ctgggagata gcgcccgtgc gcgtctgcca gctggtgcag ggcgaggcgc
     2221 gcgtggacgc gccgctcccc gggtgcgagg cgtggagccc gacagagctt cgcccgtggg
     2281 cggagctggg cctgggcacg ttctcgggga gcatcgccta tcgccgcgtc ttctcgcttt
     2341 cagaattgga agcggcccgg aaggcggtcc tcgacctggg cacggtgaag tatgccgccg
     2401 aggtgtgggt gaatggccgg gctgtgggag cgcgcctctg gccgccgttc acgttggacg
     2461 tgaccggcta cctgcgcccg gggcgcaacg agatccgggt ggtggtgacg aacacgtggg
     2521 ccaaccagtt cctccaaccg gaggagatgg cgcacgcgcg tgagcgcggg tggtacaaca
     2581 cctatgctca gcgcgtcgag ccgttccagc gcgagagcct ggaatccggc ttgcttggcc
     2641 ctgtctccct gcgcctgttt gagtaaaggt ggcgctgctg gtcggttcct gacggcctga
     2701 acggaatgcg cggccgggcg cgcgaatggt atcgccagac ccgccgcgca ttctacaggc
     2761 tctttccccg cttacctgct gtaggggcag gcgaggacgc acgtctcgaa gttacacggg
     2821 cggtgcttcg ggatcggggg ttgctgacgc aggccctcgg caagcgcttc ggcgtcaatc
     2881 ttctctggga gaggcacgtt catctcccag ccgacgtatt tcacgccctc ctcgcccacg
     2941 agcgagtagc gcttcgccca atcgcaccgg ttgcgatcca tccggtagaa ccgctcggcc
     3001 acgccgttga cctccaccgt cgcctcatcc tggaaggcgc aggtgtggca ggcatccagg
     3061 caccgccggc aatcgccgca gtcgaccggc tggagatact ctgcgggaat cgggtcggcg
     3121 gcaatcggcg catcggtgac aatggcgacg aagcggacgt tcggcccgaa ttcgggggtg
     3181 atcacgaaac cgcccttgcc gaggtggccg agcccggcgg caaccgccgt gaaccggttg
     3241 cagaatgcat ccggctgctc gccgcgcggg ttggcgacca ccgatccggt gccgcacagg
     3301 tcaaagctca tcgtcgcccg atagccgcgg tcctccagcc accggatcgc ccgatagccg
     3361 atgttgcgga ggagcttaac cgactcgtac tgcgcgaagg catacgggcc gactgcctcc
     3421 gccggcggca gcgccgtgcg ctcgacgctc gcgcgcggca ggcgcagacc cacgacgatg
     3481 accgagcgcg ctcccttcag gtgatcttcc ggcgcgcgcg tgcgcgtcac ttcggtatgc
     3541 acctccgggt catactcgcg catgcgcggt gagcggtcac gcgcgaccaa gacctcgacg
     3601 ccatcgaaca gggccgcaag ctgcgggtgc agcgccgcca ggcgctccgc cgggacgacg
     3661 ctggcgagat cggcgcccca ctcgtggaga agccgcttga gctgcgcgcg cgtctcctca
     3721 ggcgtgggcg cgggaaccga cacctgcggc aactcgcgtg aggtgggctc gagtggcgcg
     3781 gcagtgatta ccgtggccgt ttggacgctc cagccctctt tcactccggt gaacgtcgtc
     3841 tggaagctct tctccgggaa ggcggtgtcg cacacggcgc tgtagccctg gcgttccagg
     3901 gcacgcgcga tgtcataggc cgccatctcc agcaggtatt gccgcgcggg agccgccgga
     3961 tcggcgccct cgggcgtccg gaagtgaagg cccacagtga ctgccgtctg cccgtccggg
     4021 aggtactccg tcggccggat gccgatcgct tccagatcgg cctggggggt gaccatcacg
     4081 aaatcggccg cccagcggta ggcgagggag cggacctgct cctcaagccc gcggcgcgat
     4141 gcgagttcat ccggcgtgtt ccgcttccgg cgcggggcgt tggtgtagct cgagtcgaag
     4201 tagcggcgct ccttcggcag gcaatagcgg aggcagctcc ccatctcgcc gccgcggaag
     4261 ccgtggatgc gcacctgctc caggatcacc tcctcattca cgtggtcggg gatcgggagg
     4321 tccaggtcga ggtcgaagtg ctcgccccag gcgcaccgcc agaggttctt gcgcgccttc
     4381 gtatacttct tgccctcaat ccagagctcg tccaggccgt cgacttcctt cgagagcgcc
     4441 ttcgagaggc agtgcttcac gcacagcttg cacatgtcgc agacgcttcc gggctccagg
     4501 aggggcgtag gctggagcgg ggcgtcggtg atcacagtga cgtagcgctg gcgggcgcca
     4561 tactccgggg tgattgccag gccgctgtag cccagatccg ccagaccggc ggcgaccgaa
     4621 gcatagatgt ggctgatatc gggcgcgaag tgcgcggtca ggtctttgta gcccttatag
     4681 cgccagatgt tcgagctgac gatgggaacc gcggcatatc cctccttctc caggaagagg
     4741 cccatgcgat aggagatctc gtccaggcgc gtgttcatcc aatactgcac ccggtagggc
     4801 ccgatcacct gggggtgctc gatgccgccc atctcgatgc acgcgtctgg atggtggagc
     4861 gccatgacca cgaccgagcg cgccgagggg agaattccct ggggcgacat ccgaagaggt
     4921 gcgttctcga aacgctcgat gttcgcgatt ccgacgaggt ctgccccaag ctgtgaaagc
     4981 gcaaactcct tcacgcggcc ggtgagcccg gaatcgacag gccgcaccgc tcctgattga
     5041 tccttgactg ccatctcgct tctccttctg gagtgccgtg tggggccgcg ggtgtctgac
     5101 gaggggcgcc tctacggcgc tggtgcctcg cccgcgcccg tctccgtttc gcattcgagg
     5161 ccagcgatga taagctcagt ttcctccctg tactgtcatg cctgctgtcc tagccggttt
     5221 gctgctgcgg cgatctcgcg ccggatcttc tcccggtgtt caggggttgc ccgggaggcg
     5281 gggaggtgga tccccagcgc cccgaggagc tgctcacggg catcgagcac cggtaccgca
     5341 agggaataga cctgcgtgcg tccgcactcc tcctccgcca tgcccgcgcg gcggacctcg
     5401 gcgagcgccg cctccagcgt ctcctctgtc tcgatcccgt tccagctctc tcccggtagg
     5461 ccatgcaccg ccaacagctc gctcagctcc tcgggtgtcg cgtaggctgc cagcacgcgc
     5521 ccggtcacca tccggaaaaa tgcgctcagc tcctcatagg tggggctgac ccggacgaca
     5581 gcatgcccgt ccgcgcggag cagcgggtag cgccggccat gcaccagcgt ggcgaagacc
     5641 accgactccc ccgtgcgctc tgcgagcgag tgaatcgtgc cctgggccgc cgccatgaac
     5701 ttcgccgaga gagtgctccc gcgcgccagg tcgcggcacc gtgggcccag gcggtagagg
     5761 tgctgatcgt cgcgcgccac gtagccgcac atgaccatcg tcttcaggat gttgtgtgcg
     5821 gtggacggag gcactcccag gcgcccggca atcgcctgca aagagactcc ttcacctccc
     5881 agcccttgct ccatcagggc atcgagaacg tcgagcgcgc gcttgatcga ttggatcggt
     5941 acggtggctg ccatctctct gcttccagat agttgaatgc tgttccatta tatagaagcg
     6001 ccgggcccct gtcaagcggt ttttcatgca ggaggcagtg gttcct
//
LOCUS       DAOSCV010000137         6008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8367_length_6008_cov_4.155720, whole genome shotgun sequence.
ACCESSION   DAOSCV010000137 DAOSCV010000000
VERSION     DAOSCV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8367_length_6008_cov_4.155720"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLY56_04780"
     CDS             complement(<1..447)
                     /locus_tag="PLY56_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20828.1"
                     /translation="MPDCSTVINRAGDVPHRRRCRTGTKTAVGGSQKRVLCSQLVEIE
                     WEEDVQAKAKGFSLLIAGVLLALFVSAREQAPSAKSEGTPEEEPVRSAKTAPTVPKFL
                     ESGNAGCQEAANSPRENAEDQVNSLNQKEFLAAVQASDDPAEWNAPE"
     gene            810..2555
                     /gene="iorA"
                     /locus_tag="PLY56_04785"
     CDS             810..2555
                     /gene="iorA"
                     /locus_tag="PLY56_04785"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOJ20829.1"
                     /translation="MLLSGNEGIARGAYEAGARVAAGYPGTPSTEILETIARHYETIY
                     AEWAPNEKVALEVAIGAAFGGARALATMKHVGVNVAADPLLTAAYTGVNAGLVIVSAD
                     DPGMQSSQNEQDNRHYARFAKIPMLEPADSQEAKEFTLRAFALSEEFDTPVLLRTTTR
                     VNHGKSVVSLGEPVAPPERRFERDVQKYVMIPAYGRVRHVFVEERLQRLRRFAETTDL
                     NVVEMGDLAVGVITSGINYQYVKEAMPEASVLKLGLTYPLPEELIRQFASRVKTLYVV
                     EDLDPFLEDQIRAMGIQVIGKERFPSVGELDPVIVARGLGIEREQAEAPAVEDAPGRP
                     PVLCAGCPHRGFYTVLRKLKAIVTGDIGCYTLGVLPPLTTMDTCVCMGASIGNGIGIR
                     RAQAPDDTRPVVAVIGDSTFIHSGITGLIDAVYNNTPVTVCILDNRTTAMTGGQEHPA
                     SGKTLRGTEAPQLDLPGLVRAVGVEDVLQVDPYDLDAVEKALRYTTQCGKPAVIITTR
                     PCVLKRGGARPEVFTVSLEECTGCQLCFRIGCPAIESVRDENGKLKASINPAMCNGCG
                     VCFQVCRSGAIRRRE"
     gene            2687..3268
                     /locus_tag="PLY56_04790"
     CDS             2687..3268
                     /locus_tag="PLY56_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOJ20830.1"
                     /translation="MSEAVTSIRLAGVGGQGILVASEVLCDVLLACGHDVKKSEIHGM
                     AQRGGTVNSDVRFGKKVYSPVIPHGEVDVLVAFEQMEALRYLPSVKEGGVVVINAQRI
                     LPSAVASGKVAYPEDVVARVAQRAGTVISLDALSLAKDAGSLRSVNVCLLGVLSRFIA
                     VEESVWEAVLTERFREKGLAANLKAFALGRAAE"
     gene            3280..4587
                     /locus_tag="PLY56_04795"
     CDS             3280..4587
                     /locus_tag="PLY56_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ20831.1"
                     /translation="MIWNKEAECQPREEREKLQSERLRETVRRCYEHVPAYRKKLDEA
                     GIRPSDIRSIADLKYLPFTTAEDLRDNYPFGMFACPLSEVVRVHSSSGTTGKPKVVGY
                     TRNDVETWAEVMARTLACGGAGPHDIIQNAYGYGMFTGGLGIHYGAERMGATVVPISG
                     GNTKRQVMILEDFGSTMLACTPSYALVIADTAAEMGVDLHKLPLKYGVFGAEPWTDEM
                     RREIEARLGIRATDIYGLSEVIGPGVASACEAQNGLHIFDDHFVPEVIDPNTLEVLPP
                     GTQGELVFTSLTKEAFPILRYRTRDITRLSYEPCACGRTHPRMQRVAGRTDDMLIIRG
                     VNVFPSQIESALMGIEGTEPHYVIVVDRSQGRLDELEVWVEVSPELISGEVRRLELFE
                     RKVTAELTSVLGLSLKVRLVEPKTIARSEGKARRIIDRRELGS"
     gene            4584..5015
                     /locus_tag="PLY56_04800"
     CDS             4584..5015
                     /locus_tag="PLY56_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ20832.1"
                     /translation="MKVNQISVFLENKSGRLADVTRVLADHRINIRALSIADTIDYGL
                     LRLIVNDPEQAAKVLTDQGFTVAQTEVLAIEIPDRPGGLAGIIDILASQGLNIEYMYA
                     FVGRSGEHAIVIFRIEPVDKAIKALKEGGARILTGSELYAL"
     gene            5843..5986
                     /locus_tag="PLY56_04805"
     CDS             5843..5986
                     /locus_tag="PLY56_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20833.1"
                     /translation="MPIWNERCETMQRADLEQLQLERLQATVNRVYRNVPFYQRRVEE
                     LSV"
BASE COUNT         1087 a         1755 c         2091 g         1075 t
ORIGIN      
        1 ctcgggcgcg ttccactccg ccgggtcgtc cgatgcttgc acggcagcca ggaactcctt
       61 ttgattcagg gaattgacct ggtcctccgc gttctcgcgc ggggaattcg ccgcttcctg
      121 gcaaccggcg tttccagatt caagaaactt gggcacggtc ggggctgtct tggccgaccg
      181 gaccggctct tcttcgggcg ttccctcgct ctttgccgag ggcgcctgct cgcgggcaga
      241 gacaaagagt gccagcagca cgcccgcgat caaaagagag aaacctttgg ccttcgcctg
      301 cacatcctcc tcccattcga tttccacgag ctgggagcag agcacgcgct tctggctccc
      361 gcccactgct gtcttcgtcc cggtgcgaca ccgtcggcga tgaggtacat caccagcgcg
      421 gttgatcacg gtggaacaat cgggcatctc gccccctcct tagtggtgcg ggggttttcg
      481 cgctcgccgc gcccgtggac tcggcgtcgt cactcctgtc tacgcgcggg gaaaaaaggt
      541 gcctctgaga aaagtactca tcacgccgag cggctcgggc tgacgcgagg tgatcatgct
      601 ggaggcgtgc gcaggtgaaa acacctgcgc tggttttgtc gcgcgggcgg ttgcgccgcg
      661 ctgagaatct ctatcattga cgaatggtga aaagcccgtt atactgggtg gagcgggtgc
      721 cctgccgata gccgcggtcc cgccccacgt gactgccccg tgcagcctcg gatcagtttg
      781 cggaggttta tcaatccttg agtaaaacca tgctgctctc cggaaatgag ggaatcgcgc
      841 gtggcgcgta tgaggccggc gcgcgcgttg ccgccggcta tcccggcacg ccgagcaccg
      901 aaatcctgga gacaatcgcc cgacactacg agaccatcta tgccgagtgg gctccgaacg
      961 agaaggtggc gctcgaagtc gccatcggag ccgcgttcgg cggcgcgcgg gcgctggcga
     1021 cgatgaagca cgtcggcgtc aacgtcgcag ccgaccccct cctcaccgcg gcctacaccg
     1081 gcgtgaatgc cggcctggtg attgtcagcg cggacgaccc cggcatgcag agctcgcaga
     1141 atgagcagga caaccggcat tacgcgcgct ttgctaagat tccgatgctc gaacccgcgg
     1201 atagccagga ggcgaaggag ttcacgctcc gcgcgttcgc gctcagcgag gagttcgaca
     1261 cgccggtgct cctgcgaacc accacccggg tgaaccacgg caagtcggtg gtttccttgg
     1321 gcgagccggt ggcgccaccg gagcgccgct tcgagcgaga cgtgcagaag tacgtgatga
     1381 tccccgccta cgggcgggtg cgccacgttt tcgtagagga acgcctgcag cgcctgcgcc
     1441 ggtttgccga gaccaccgat ctcaacgtgg ttgagatggg cgacctggcg gtgggagtca
     1501 ttacgtccgg catcaactac cagtacgtga aggaggcgat gcccgaggct tctgtgctga
     1561 agctggggct cacctatccg cttcccgagg agctaatccg gcagtttgcc tcgcgcgtga
     1621 agacgcttta cgtcgtggag gatctcgacc cgttccttga ggatcagatc cgggcgatgg
     1681 ggatccaggt gattggcaag gagcggttcc catccgtggg cgagctggat ccggtgatcg
     1741 tggccagggg cctggggatc gaacgcgagc aggcggaggc gccggcggtc gaggacgcgc
     1801 ccggccggcc tcccgtgctc tgcgcggggt gcccgcaccg cggcttctac acggtgttgc
     1861 gcaaactgaa ggcgattgtc acgggcgata ttggttgcta cactctgggc gtgctcccgc
     1921 cactgacgac gatggacacc tgcgtctgca tgggggcgtc catcggaaac ggcatcggga
     1981 tccgccgcgc gcaggcgccg gacgacacgc gccccgtggt cgccgtgatc ggcgactcca
     2041 cgttcatcca ctcggggatc accgggctca ttgacgccgt ctataacaac acgccggtca
     2101 ccgtctgcat cctcgataac cggaccacgg cgatgaccgg cgggcaggag catcccgctt
     2161 ctggcaagac gctgcgcggg accgaggcgc cgcaactcga tttgccgggc cttgtgcgcg
     2221 cggtgggcgt cgaggatgtg ctgcaggtcg atccgtacga cctggatgcc gtggagaagg
     2281 cgctgcgcta tacgactcag tgtggaaagc cggcggtgat catcaccacg cggccgtgcg
     2341 tgctgaagcg cgggggcgca cgcccggaag tgttcacggt atcgttggag gaatgcaccg
     2401 gctgccagct ctgcttccgc atcgggtgtc cggcgatcga gtcggtccgg gatgagaatg
     2461 ggaagctgaa ggcgtcgatc aacccggcga tgtgcaacgg ctgcggtgtc tgcttccagg
     2521 tgtgccggtc tggggcgatc cggcgccgcg aataggcaag aagcggcatc ggccggggcg
     2581 cgcggagtgc gcgatgaggc cgggtggaga ttttgtctcc tcccactgat gattccaccc
     2641 ctgcacgcga gtgttgggct cttgagagaa tacgaaagcg gcaggaatgt ctgaagcggt
     2701 aacaagtatc cgattggctg gcgtgggtgg ccagggcatc ctggtcgcca gcgaggtgct
     2761 atgcgacgtc ttgctggcct gcgggcacga cgtgaagaag agtgaaatcc acggcatggc
     2821 ccagcgcggg ggcacggtga acagcgacgt gcgctttggg aagaaggttt actcgccggt
     2881 tatcccgcac ggcgaggtgg atgtgctggt ggccttcgag cagatggagg ctttgcgcta
     2941 cctcccttcc gtgaaggagg ggggcgtcgt cgtgatcaac gcccagcgta tcctcccctc
     3001 ggcggtggca tcggggaagg tcgcatatcc cgaggatgtg gtggcgagag tggcgcagcg
     3061 cgcgggcacc gtgatctcgt tggatgccct tagcctcgcc aaggacgcag gatcgctgcg
     3121 gagtgtgaat gtgtgcctgt tgggggtgct gtctcgcttt atcgcggtgg aagagagcgt
     3181 gtgggaagcg gtgctcacag agcgattccg cgagaaggga ctggcggcca acctgaaggc
     3241 attcgctctg ggccgggcgg ccgaatgacg gagaagagga tgatctggaa caaggaagcc
     3301 gaatgccagc cacgcgagga gcgagagaaa ctccagagtg aacgcctccg ggagacggtg
     3361 cggcgctgct acgagcatgt gccagcctac cggaagaagc tggacgaagc ggggatccgc
     3421 ccaagcgaca tccgctccat cgccgatctg aaatacctcc cctttaccac agcggaagat
     3481 ctacgcgaca actatccctt cggcatgttt gcctgcccgc tgagcgaggt ggttcgcgtt
     3541 cactcctctt cgggcacgac aggcaagccc aaggtcgtgg gctatacccg gaacgacgtg
     3601 gagacgtggg cggaggtgat ggcgcgcacg ctcgcctgtg gtggcgccgg gccgcacgat
     3661 atcatccaga acgcctacgg ctacgggatg ttcacgggcg gcctggggat ccactacggc
     3721 gccgagagga tgggggcgac cgtggtgccg atttccggcg gcaacaccaa gcggcaggtg
     3781 atgatcctgg aggattttgg gagcacgatg ctggcgtgca cgccctccta tgccctggtg
     3841 atcgccgata ccgccgccga gatgggcgtc gatctccata agctcccgtt gaagtatggc
     3901 gtcttcggcg cggagccgtg gaccgatgag atgcgccggg agatcgaggc gcgcctgggg
     3961 attcgggcaa ccgacatcta tgggctgagc gaggtgattg gcccgggggt ggcgagcgct
     4021 tgcgaagccc agaacggcct ccatatcttc gatgaccact ttgttccgga agtgattgac
     4081 ccgaacacgc tggaagtgtt gccccccgga acgcagggag agttggtctt tacctcgctg
     4141 acgaaggagg ccttccccat ccttcgctac cgaacgcgcg acatcacgcg gctttcttat
     4201 gagccgtgcg catgtggtcg aacacacccg cgaatgcagc gagttgcggg gcgcaccgac
     4261 gacatgctga tcattcgggg cgtgaacgtc ttcccgtcgc agatcgagag cgccctgatg
     4321 gggatcgagg gcacggagcc acactacgtg atcgtggtgg atcgcagcca gggccgattg
     4381 gatgagctgg aggtctgggt tgaggtctcg ccagagctga tctcgggcga ggtgcggcgg
     4441 ctggagctct tcgagcgaaa ggtgacggcc gagctgacga gcgtcctcgg gctctccctg
     4501 aaggtgcgtc tggtcgagcc gaagacgatt gcgcgcagcg agggaaaggc gcgcagaatc
     4561 atcgacagga gggagctggg ctcatgaagg tgaaccagat ttcggtgttc ctggagaaca
     4621 agtcgggcag actggcggat gtcacgcggg tacttgccga tcaccggatc aacattcgcg
     4681 cgctctccat cgccgataca atcgactatg gtctgttgcg cctcattgtg aacgatcccg
     4741 aacaggcggc gaaggtcctc acggatcagg ggttcaccgt ggcccagacg gaggtcctgg
     4801 cgatcgagat tccggaccga cccgggggcc tggcgggcat catcgatatt ctggcatccc
     4861 aggggctgaa catcgagtac atgtacgcct ttgtggggcg ctccggggag cacgccatcg
     4921 tgatcttccg gattgagcct gtggataagg cgatcaaggc gctcaaagaa ggtggggcga
     4981 gaatcctgac cggcagcgaa ctgtacgcgc tgtgacctgg atcctctcca cacggtcggt
     5041 cgcacaggta gctgcgccgg cgcatggtct acatcgcgca tgacaccggg catgagcgat
     5101 gacgcgccct atctccgatg ggtgatgggc gcgcgggcct aaagcagcga taggcggtgc
     5161 ggcactcagt cgtctccgct ccgccctgtg cggccgctgg atgagcgaga tacgatcaga
     5221 aggatcaccc attccaggaa gcaggcgccg atatcggtgc ctgcttcctg ctgacgggag
     5281 tggccatcga agtagagaaa cgaagcgtgt gggggcgata gccgcctcga gcgcaacgac
     5341 tcggtgattg cccggcaccg agccccgccc gacgggcacc ctcctcgctg tggccagcct
     5401 gaacgcggag gcgcagcatc ccctcaccgc ccgctcagct gcaattcaga tcgcttgcgc
     5461 tccagggcgt gcattgtacg cccggctgaa ccagtggggc gccgggtatc tctggcaagc
     5521 ggtttcccgt ggtgtctggg gtcgaagagc gtgcccggcc gtgcgtgagg catgacagcg
     5581 gcccgcagcg acgagagagc ggtttgagac gaagccgggc gggagcgagc atcccgcgag
     5641 tgggatacaa gtcctggggc gccggcttcg tgcattttgc cgttctacga gggcggcaag
     5701 tatctgccgg gcgaggatgg gtggtgcatc aggcgcctgt tggggattgg accggtcatc
     5761 cttcggggga agggtggaga gtgcaatgcg ccgcgctgac cgtgggaaag gcgacgcgct
     5821 tctgatagag gagtaagaga aagtgcctat ctggaatgag cgttgtgaga caatgcagcg
     5881 agccgatctg gagcagctgc agctggagcg gcttcaggca acggtgaacc gggtttatcg
     5941 gaatgtgccc ttctaccagc gccgggttga ggaactgagc gtgtagccgg aggagatccg
     6001 gtcactgg
//
LOCUS       DAOSCV010000138         6003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8374_length_6003_cov_3.554976, whole genome shotgun sequence.
ACCESSION   DAOSCV010000138 DAOSCV010000000
VERSION     DAOSCV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_6003_cov_3.554976"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="PLY56_04810"
     CDS             complement(<1..1656)
                     /locus_tag="PLY56_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOJ20834.1"
                     /translation="MKYAAGVDGGSSAIKAVLVHEDGAVETVALLPVGSRPLHTAREV
                     LQALRDRVSSSKIRLGVTGTAGRALCQALGIEALDDSVTLAAACSARYPAVRSIVEMG
                     MESQRFLTLAPDELTGRLRIEESNLGHKCAAGSGSFLEHMRKRLNYGSIEEFAQVAAE
                     TEPPATLSGRCSVFTESDIVHLYQKGTPRERIAAGIHQAICRNYRAAMVKGIAIRPKV
                     AVVGGVALNPAVIKYLRIELGVEEDALFVPEDARYLNAIGAALRADAIVDLDEACRLL
                     DARLAQPISYPVAPRLTLERSEILQPPAQQLPACEIPLAALGVDIGSVSTKAALITRI
                     DGQTHVLAAYYRRTNGDPLAAVRDTLAQIQAQVRERGYSIGRVVAATTGSGRYLTADY
                     IGAELIKNEITAQAHGALAFIPDVDSVFEIGGQDSKYIRFENGVIVDFEMNRACAAGT
                     GAFLEKQAANLGIPLEEFGSRALAGKNPPELDWQCTVFSESAAHHYRQNNVPVEDLAA
                     AICLASVRNYLNKNVGNRPIGKVVAFQGAVAFNRGMVAAYETML"
     gene            complement(1948..2625)
                     /gene="phoU"
                     /locus_tag="PLY56_04815"
     CDS             complement(1948..2625)
                     /gene="phoU"
                     /locus_tag="PLY56_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOJ20835.1"
                     /translation="MQPEVARGVRRTFEDHLSDLLDNVLRMGAAVEEMVARAVEALRH
                     GDVTAGEKLDQFDDVVDEYNLNIENECLQLIALQQPMARDLRTIAATLKIITDIERVG
                     DYAVDVAKTAAKLAERPLFKPLVDIPRMGQLVQEMLRDVLESFVNRDIERIRGVIQVD
                     DAVDALNRQLHDELIGYIEKDPSIAHQAVWLILVARYLERMADHITNIGERVHYMETG
                     ELKELHK"
     gene            complement(2665..3474)
                     /gene="pstB"
                     /locus_tag="PLY56_04820"
     CDS             complement(2665..3474)
                     /gene="pstB"
                     /locus_tag="PLY56_04820"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOJ20836.1"
                     /translation="MTEPRPIFQRPNETRALQGEVKIGVRGLNLFYGRFHALKGVTLD
                     IREKAITALIGPSGCGKSTFLRTLNRMNDLITNVQVQGKVEIDGEDIYGPKMDVVDLR
                     KRVGMVFQRPNPFPMSIFDNVAYGPRTHGVRDKHRLAEIVETSLVRAALWDEVKDKLR
                     ESALALSLGQQQRLCIARVLAVDSEVVLMDEPCSALDPISTLHVEELMRSLRENYTIV
                     IVTHNMQQAARVSDETGFFLNGEMIEFGPSFEIFTTPRDKRTEDYVTGRFG"
BASE COUNT         1123 a         1968 c         1791 g         1121 t
ORIGIN      
        1 gagcatcgtc tcgtaagcgg ccaccatgcc gcggttgaag gcgacggcgc cctggaaagc
       61 cactaccttg ccgatggggc gattgccgac gttcttgttg aggtagttgc gcaccgaagc
      121 caggcagata gcggcagcga gatcctcgac cggcacgtta ttctgccggt agtgatgcgc
      181 ggccgactcc gagaaaacgg tacattgcca gtccaactcc ggcgggttct tgccggcgag
      241 ggcgcggctg ccaaactcct ccaggggaat gccgaggttg gctgcctgct tctccagaaa
      301 ggcgcccgtg cccgcggcgc acgccctatt catctcgaag tcaacgataa ctccattctc
      361 aaagcggata tacttggagt cctgcccgcc aatctcgaag acggagtcga catccgggat
      421 gaacgcgaga gcaccatgtg cctgcgctgt gatctcattc ttgatcagct cggcgccaat
      481 gtaatcagcg gtgaggtagc ggccagaacc ggtggtggcc gcaacgacgc gcccgatgga
      541 atagccacgc tcgcggacct gcgcctggat ctgggcaagg gtgtcgcgca ctgcggcgag
      601 cgggtcgcca ttggtgcgcc ggtagtaggc ggcaagcacg tgtgtctgcc catcgatccg
      661 ggtaatgagc gccgccttgg tactcacgga gccgatatcg acgcccagag ccgccagcgg
      721 gatctcgcac gccggaagct gctgggcggg cggctgaagg atctccgatc gctcgagcgt
      781 cagacggggt gcgacgggat aggagatcgg ctgggccagc cgggcatcca gaagccggca
      841 cgcctcgtcc agatcgacga tggcgtccgc gcgcagcgcc gcgccgattg cgttcaggta
      901 gcgcgcatcc tccggaacga agagcgcgtc ctcttccact ccgagttcaa tccgaaggta
      961 cttgatgacg gctgggttga gggcgacgcc accgacgacg gcgactttgg gccgaatcgc
     1021 gatgcccttg accatggccg cgcgatagtt tcggcagatc gcctggtgga ttcccgcagc
     1081 gatgcgctcg cgcggagttc ccttctgata gaggtggacg atgtcggact cggtaaagac
     1141 gctacagcgc ccagagagag ttgcgggcgg ctcggtctcg gccgcgacct gcgcgaactc
     1201 ctcgatggag ccatagttga ggcgcttgcg catatgctcc aggaaggagc cggaaccagc
     1261 cgcgcatttg tggccgaggt tactctcctc aatgcggagt ctgcccgtca gctcatcggg
     1321 cgccagggtc aggaaccgtt gcgattccat ccccatctcg acgatggaac gcacagccgg
     1381 atagcgggcg ctgcaggctg cagcgagagt gaccgaatcg tcgagcgctt cgatgcccag
     1441 ggcctggcag agcgcgcgcc cagcagtacc cgtgactccc agacggatct tggacgacga
     1501 aacgcggtcg cgtagggctt gcagaacttc cctggcagta tgcagcggtc ggctgccaac
     1561 aggcaagaga gcaacggtct ccaccgcccc gtcctcatgg acgaggaccg ccttgatggc
     1621 gctggaaccg ccatcaacgc cggcggcata tttcatagtg catcctcctg gctcacctca
     1681 gctgatggcc tcaggcgcgg ggcagaacag gagaagtgac gcgcccgggc ctctctctat
     1741 cgcctattcg ccccccttcc gctggactcc tgccgcttcc ccttcaagcg gtcgttgtgc
     1801 aagcgcatcc gtggaaggag aagggatggc ccatgaggag gggcagagcg agaacggcgt
     1861 acaggcgcgg gcgacaagga ggagtcatgc gaaagcggcg gcagtcagga agcgcaggga
     1921 ctgccgccgc cggaagggaa cggcgtgcta cttatggagc tctttcagct cgccggtttc
     1981 catatagtgg acacgctcgc caatattggt gatgtgatcg gccatgcgct ccaggtagcg
     2041 cgcgacgagg atgagccaca ccgcctggtg agcaatgctt gggtctttct ctatatagcc
     2101 gatcagctcg tcgtggagct gacggttgag ggcatcaacg gcatcatcga cctgaatgac
     2161 gccgcggatc cgctcgatgt cacgattgac aaagctctcg aggacatcgc ggagcatctc
     2221 ctggacaagc tggcccatac gcgggatatc gaccaggggt ttgaagagcg ggcgctccgc
     2281 gagctttgct gccgtcttcg ccacgtccac cgcatagtca ccaacgcgct caatgtcggt
     2341 gatgatttta agggtcgcgg caatcgtgcg aaggtctcgc gccatcggct gctgcaaagc
     2401 gatcagctgg aggcactcgt tctcaatgtt caggttgtac tcgtctacga cgtcatcgaa
     2461 ttgatccagc ttctcgcccg cagtgacatc accatggcgc agtgcctcga cggcacgcgc
     2521 caccatctct tcgactgctg cgcccatgcg gaggacgtta tcgaggagat cgctgaggtg
     2581 atcctcgaag gtccgccgca caccacgcgc tacttcaggc tgcatgccaa gcccctcctc
     2641 gtccagcgga atgcgtgtcg cagtttaccc aaaccgacct gtcacataat cctccgtgcg
     2701 cttgtcacgc ggagtggtga agatctcaaa ggacggcccg aactcgatca tctccccatt
     2761 caaaaagaac ccagtttcat ccgaaacgcg agccgcttgc tgcatgttgt gcgtgacgat
     2821 gacgatggtg tagttttcac ggaggctccg catcaactcc tcgacatgca gcgtcgagat
     2881 cgggtcgaga gccgagcacg gctcatccat gaggacgacc tccgagtcga ccgcaagaac
     2941 acgcgcgatg cagagccgct gttgctgccc cagggacagc gcgagcgccg actcgcgaag
     3001 cttgtccttg acctcatccc agagagcagc gcgcaccagg cttgtctcga cgatctccgc
     3061 gaggcgatgc ttatcacgaa ccccgtgggt tcgcggacca tacgcgacgt tgtcgaagat
     3121 cgacatgggg aacgggttcg gacgctgaaa caccatgccg acacgctttc gcaggtcgac
     3181 aacgtccatc tttggcccgt agatatcctc cccgtcaatc tccactttgc cctgaacctg
     3241 aacattggtg atgaggtcgt tcatgcggtt gagagtccgc agaaacgttg actttccaca
     3301 tcccgatggt ccgatcaagg cggtgatcgc cttttcgcgg atatcgaggg tgactccttt
     3361 cagagcatga aaccttccgt agaaaagatt cagcccgcgc acgccaatct tgacctcgcc
     3421 ctgcaaagcg cgggtctcgt tggggcgctg gaagatcggt ctgggttctg tcatcgtggc
     3481 ttccataggt ggtccttcac cacagatgcg cactgcgagc gtgcgctcgg gtcagggagg
     3541 taaccgtggt tatcccgagt ctcaccttag acaggacaca cggggcaccc ttttggctcc
     3601 tggacgatac atccgggacc tgggtcgaga cgatccgccg gaggggcctg cctgcaccct
     3661 cgcctagtcg aggcgagacg ccggaggagc ctgcctgcac cctcgcctgg tcgaggcgag
     3721 acgccgatag agagcgcgcc atgaccgcaa tcgaagtcac tgccaccgct ctggccggac
     3781 ggtagcaccc cgccataaga ggcggcctca gcataagcgc gccgcaccgg tccatcacgg
     3841 ctcctattca ggacgctctt ctccctcgag gcgagtggtc cgcaaccgta aagagccccg
     3901 ctctctgccc ggcctcgcca gaggcgtcgt tcgcccgtgt ttcatcatac cacgccgaca
     3961 ttaatagaag cttagccgca ccttaacacc accttaaatg cgccggcctt cgaccaggct
     4021 catcccccgg cacacgcgct gagtatttct tgattccctg ccgcagacgc ccatgttccc
     4081 ccgtctgcgt cgcgccaggc tgccaatgac ccggggcgcg gcctggatct gcatatcgcg
     4141 gtaccgggcg actggcggaa accctcggcg gcaatcgctt cagaccgtcg catcctgtag
     4201 cgatgccgca tttctgtgtg cagcgcctgg aagggtggca tgcacgccgt tcccactgtc
     4261 atccggacag caccggccca atgcgttttc tcttcgggtc acgcgacagc accacccact
     4321 gcccccttgc atggcatcgc tcatctcgcc tgcagggttc ttctgacctc acacaggagg
     4381 ttcgtgacgg agtgacgaac ctggtcaata ccgcctggcc aatggctggg gcatcgtgcc
     4441 ccgaccgcat tgacgtgatg gcctggcaca gccctcggaa cccgatccgc acgttcgctt
     4501 cccgttccgc gcaaggcgat cgggcatggg ccgtgcaggt gagagagaga agggagtgtg
     4561 gtatgcgtaa agcggcaaaa ccaggtgcgg gttctcgaca acgatcacgg catggcggtg
     4621 gtggccgccg gcaggtcgag gtccccacgc gttattgctc ccggtgtgga cagcagatgg
     4681 aggtcgtacg tgcggtaccc ggcgagccga gttggcgcgc gcagtttcgc tgcaccaatc
     4741 cggaatgtgg aaagtagcat ccttgattct tgcccgttcg tgtgatcgca agcctcgcgg
     4801 gtgccgatga ctggtagtcg cgaatgggcc aggcgacagc gattgttggt ccatgacacc
     4861 ttccgcggat gcgcactcta agtgtgcgca gcgatcgcgg gcgatgggtg aacccgggtt
     4921 cacccatcgc tgtgcgggcg tccccctctc cccctggcgg tcactccacc cactgccctg
     4981 atcccgactc ttctccccac cggcttctcc catcccgagc cattccatct cccaggatcc
     5041 cgtcgcgcgc gcgcttctta tgcgcgatgt atcaggcccg cgttgccgcg cgccactggc
     5101 acactactgt ttgccattcg gcggactcct cttgctccgg cctgtggcac cctgtctcgg
     5161 agaaagcccg cgttgccccg ctgtcatgtg atgatcgctt ttcaggacac ttctgacaca
     5221 gcctctggcc gcacgaaccc cccgatcgcc attgcgcccc cgaggagatc acctcgacac
     5281 cggctccatg gatacgcagc cggcgcttgg cctccctccg gaccctcgaa agccccctct
     5341 tctggcccgt ttttcgggcg tccctcgttt tccaaagccc cagtcaggcc ccctcgtccg
     5401 cccgattgcc ccaaaaagcc cctctttcag gcaggtttca cccctcacag gcctttctga
     5461 cgcccttaga gctccaaaaa cgcgctcgtt tcacgtggaa cgggcccgaa tcacgcccgc
     5521 acgcccctct ccccgccgct ccgtacccct cacgaggcag tacgacgcta tagaatgcac
     5581 tcgttgaccc acgatccagc ttcctgcccc acgcgacgcg cacgcgcgca cgctaagccc
     5641 tatgcgtacg ctttcggatt cagcgtatac gatgccgcgt gttccatcag cagaggatgc
     5701 cggctgcaaa gacacgcctg gccaaaggag ccgatgcatc ctgcggcacc cggcgctggg
     5761 acgacaaacg aatccctctc tccctgcccc cgtgttccag acccggcggt gagcgcgtag
     5821 atcgcccaaa tccgccggag ctctagaaga agcgtctcct gcatccctgc atctcacacc
     5881 gcgatcggca ctccaccgga agacccggat cacttcacag ggagaaggtc gcaagcagat
     5941 gccgtgcgcc agttgccgta tcgcactgct acaggagatc cttcggggaa cgcggcggct
     6001 acg
//
LOCUS       DAOSCV010000139         5993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8392_length_5993_cov_3.480802, whole genome shotgun sequence.
ACCESSION   DAOSCV010000139 DAOSCV010000000
VERSION     DAOSCV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8392_length_5993_cov_3.480802"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PLY56_04825"
     CDS             <1..851
                     /locus_tag="PLY56_04825"
                     /inference="COORDINATES: protein motif:HMM:NF028211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ20837.1"
                     /translation="TRHAIVLQARRDGRIFFAVPWEGNTLIGTTDTDYEESPDQVTAL
                     PDEVRYLVEETRRVFPHADLGHVFATTAGVRALVRSRKKAESDVSRRHRIVDHAREGT
                     PGLLSLFGGKITAYRAIAAEAVDLVGRILGRRMPCRTAVEPLPGGSEAPEVTRCRLAP
                     RASAIGLDEEQVEHLVGLYGTRAAEVLALAEADARLRERITPGHPEILAQIHHACRAE
                     MAVTPADFLLRRTGIGFRPDHGLDALERVSREFALLLGWSAEHEREECRRYEAAADYL
                     SAGIEP"
     gene            889..1944
                     /locus_tag="PLY56_04830"
     CDS             889..1944
                     /locus_tag="PLY56_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOJ20838.1"
                     /translation="MPFVSAEELTEIAENALAAYGVPTDEARVVARSLVASNLAGHDS
                     HGVMRLPQYLEAIRAGSVAPGAPIEVIRETPSTAVLDAHWGFGQVAATRAAEIAVAKA
                     EACSLAGVAVHNSTHIGRLGEYVEIAAARGMVGMLFVNGHGGAHNTVPWGGADARLCT
                     NPLACAIPTGDAPIVIDISTSAVAEGKVRTYRIQGKPVPEGWIVDSDGNPTRDPNVLY
                     QQPPGSLLPLGGNVGYKGFALGLMVDVLAGALSGAGCSSPDARRGGNAFTFLAIDPRA
                     FCGRDEFAAFAASLISWVKSARLAPGAREILVPGEVERREAERRQKEGIPVPDEVWEQ
                     LTRLRSPAPTQTSGTRA"
     gene            complement(2082..2864)
                     /locus_tag="PLY56_04835"
     CDS             complement(2082..2864)
                     /locus_tag="PLY56_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20839.1"
                     /translation="MLPTVDFCGLTVTRLIIGANPFGGFSHQSKERDQEMVAYHTPER
                     IRETWARAEAAGINTMITNNETPHVIRTVEEYLAEGGRLQWIAQVANRTYASMFEAID
                     KAVAIGAKALYFHGGLVDNLYAERDEATLRSWIEHARGHDIPVGVAGHSTETHDWVDS
                     LDLVDFHVVCFFNCGSLHSGKGHKFKLADAFAAGQCIQRLAKPCIAYKIMAAGRIDPV
                     MAFEYAFETIKPGDVVNVGMHRGDKDNMVEENVALVKQILGA"
     gene            complement(3389..4297)
                     /locus_tag="PLY56_04840"
     CDS             complement(3389..4297)
                     /locus_tag="PLY56_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ20840.1"
                     /translation="MIRLGFVAGAGIYHAVQFAKMFNGLNEEFKHLDPYGGRPPMARI
                     EGATVVKVFDENRQHAENLSRFANVPVVASSLEEMLEGVDAIYIGDDLTMKQYQYARP
                     FIEKGIPTFIDKPMADSVAAARELMDLARWRKCLLMSSSALKYTKEFAPVTSGEADVG
                     EIVMACTLGPADLRLSRPFLFYGMHAVTLGHTLINSRPVEVFDVGERGRTAVQVKYLN
                     GAQLTVMCPHGVPVGFQGLVQGTRGSFYAKVADSAHYYSTMLKEFLAMVEKGEQTFDL
                     LQAVEVIQICCAAEESVRTGRPVRLG"
     gene            complement(4334..>5993)
                     /locus_tag="PLY56_04845"
     CDS             complement(4334..>5993)
                     /locus_tag="PLY56_04845"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ20841.1"
                     /translation="EYQIRYEYRWGAIRLCQRFRFPGDGARVRRLTLLQWKLRPELGS
                     FAVRPGAPAEASAWLGAFGVCQWGRFTPGASFDCPYESRFVPRYVACAEQGREGLEWF
                     AGSKLSQWDYQACGRPGYGHLYLGPQSRPPAVVCTISPVNIPSGAVPMAGDYTFDSWL
                     GIPVLSGRAHRPFLHRSFNRHDWPTREKIRAWAEAGVRTAHFHHDGDTFRDGLFWRDG
                     SYPPFGPEDMKEYDRVIRELQEHGIRVATYFSNKELHPTTAAYQAHGAEWARLPGERG
                     QQLHNAYAGDEYGAQMCLRSGWLAQLKENIDTVLRNHPLDGVYYDWNVALYCHNPAHE
                     PDAEPGELPDYGGWALSPVGHWDVDELVELMEWTRQRVGRDGLVIIHNTMVPCAVTEN
                     LADYVVAMEWGYGRLATAAPPLASLPLEWSFMGARPRGVIGYGCLERDAPESVHRQMT
                     LRCLLTGTAPWPAQDLDLELFAPLRDYDLSQYRFYDWRSAPVRLEEEGVAAAAYIGAR
                     EALVVVGNLTGEARTARGALASGESFAVELPPDGATVVRIPLDR"
BASE COUNT          999 a         2082 c         1932 g          980 t
ORIGIN      
        1 tcacacggca cgccatcgtg ctccaggctc ggcgcgacgg gcgtatcttc ttcgccgtgc
       61 cgtgggaagg caacacgctc atcggcacca ccgacactga ttacgaggaa agcccagacc
      121 aggtcaccgc cctcccggac gaggtgcgct acctggtgga ggagacacgc cgcgtcttcc
      181 cgcatgccga cctcggccac gtcttcgcga ccactgccgg cgtgcgcgcg ctggtgcgct
      241 ctcggaagaa ggcggagtcg gacgtctcgc gccggcatcg gattgtcgac cacgcccgag
      301 agggtacgcc cgggctcctc tccctcttcg gcgggaagat cacggcctat cgcgccatcg
      361 ctgccgaggc cgtcgatctc gtcggacgca tcctcgggcg ccggatgccc tgccggaccg
      421 ccgtcgagcc gctcccgggc ggcagcgaag ccccagaggt cacgcgctgc cggcttgccc
      481 ctcgcgcttc cgcgattggc cttgatgagg agcaggtcga gcatctggtt gggctctatg
      541 gcacgcgcgc cgcggaggtg ctggccctgg ccgaagccga cgcgcgcctg cgcgagcgga
      601 ttaccccggg ccatcccgaa attctcgccc agatccacca cgcctgtcgc gcggagatgg
      661 ccgtcacgcc cgccgacttc ctcctccggc gcaccgggat cggtttccgc ccggatcacg
      721 gcctcgacgc gctggagcgg gtctcgcgcg aattcgccct gctcctcggc tggagcgcgg
      781 agcacgagcg cgaggagtgc cgccgatatg aagccgccgc cgactacctg agcgccggaa
      841 tcgagccctg acgcatcgcg tggccacgta gccggaagag gagttgccat gccgttcgta
      901 tccgcggaag agttgacgga gatcgccgag aatgccctgg cggcatacgg agtgcccacg
      961 gacgaggcgc gggtcgtggc ccggtcgttg gtcgcctcca acctggccgg gcacgactct
     1021 cacggcgtga tgcgcctgcc gcagtacctg gaggcgatcc gggcagggag cgttgccccg
     1081 ggcgcgccca tcgaggtaat ccgtgaaacg cccagcacag cggtgctcga cgcccactgg
     1141 ggcttcgggc aggtggcggc gacgcgcgcc gctgagatag cggtcgccaa ggcggaggcg
     1201 tgttccctcg ccggcgtggc ggtgcacaac tccacgcaca tcggccggct gggggagtac
     1261 gtggagatcg ctgccgcacg cggcatggtc gggatgctct tcgtgaacgg gcatggcggg
     1321 gcgcacaaca cggtgccctg gggcggcgcc gacgcccggc tctgcaccaa cccgctcgcc
     1381 tgcgccatcc ccacggggga cgcccccatc gtcatcgaca tcagcacctc cgcggtcgcc
     1441 gaagggaagg tgcgaactta ccgcatccag ggaaagccgg tccccgaggg ctggattgtc
     1501 gatagcgacg ggaatccgac gcgcgacccc aacgtcctct accagcaacc gcccggttcg
     1561 ctcctgccgc tagggggcaa cgttggctac aagggcttcg cgctaggcct aatggtggat
     1621 gtgctggccg gcgcgctcag tggcgccgga tgcagcagtc ctgatgccag gaggggcggg
     1681 aatgccttca ccttcctggc catcgaccca cgcgccttct gcgggcgcga cgagttcgcc
     1741 gcgttcgccg ccagcctgat ctcctgggtg aagagcgcgc gcctggcgcc cggcgcgcga
     1801 gagatcctgg ttccgggcga ggtcgagcgc cgcgaggcag aacgccgcca gaaagagggc
     1861 atccccgtgc ccgatgaggt ctgggagcag ctcacgcgcc tgcgatcccc tgccccgact
     1921 cagacgtccg gtacccgcgc ctgaggctca caaaccgcgg ccacgcgggt ggccattctc
     1981 cgaccccgcc gagtccctcg acagcctcgc aggttgcgcc ccccgccccc gacagacgtc
     2041 ttccgagagc ggcgcccggg tgtcgtcccc tgacggatgg cctatgcccc gaggatctgt
     2101 ttaacgaggg cgacgttctc ctcgaccatg ttatctttgt cgccgcggtg catgcccacg
     2161 ttcacaacgt cgcccggctt gatcgtctcg aaggcatact cgaacgccat gactgggtcg
     2221 atgcggccgg ctgccatgat cttgtaggcg atgcacggct ttgccaggcg ttggatgcac
     2281 tggccggcgg cgaaggcatc agccagcttg aacttatgcc ccttgccgct gtgcaggctg
     2341 ccgcagttga agaagcagac gacgtggaag tctaccaagt ccaggctatc cacccaatcg
     2401 tgcgtctcgg tcgaatgccc ggcgacgccg accggaatgt cgtggccacg ggcgtgctcg
     2461 atccaggagc gaagggttgc ctcgtcacgc tcggcataga ggttatccac caggccgcca
     2521 tggaaataga gggctttggc gccaatagcc acggctttat cgatcgcctc gaacatgctg
     2581 gcgtaggtgc ggttggcaac ctgcgcgatc cactggagcc ttccgccctc tgccagatac
     2641 tcctccaccg tgcggatcac gtgaggcgtc tcgttgttgg tgatcatcgt gttgatcccc
     2701 gccgcctcgg cgcgcgccca cgtctcccga atccgctccg gcgtgtggta ggccaccatc
     2761 tcctggtcgc gctccttgct ttgatggctg aagcccccaa aggggttcgc cccgataatc
     2821 agccgggtca cggtcagacc gcaaaagtcg actgtcggca acatctctcc cgccccttct
     2881 acaccaacgt ccacgcgaaa ggccgactgc cccttcctcc agcgtggcgg cgcggcatcc
     2941 agagcgccag catatctctc gattccacgg ggcagcgccc gattcccgcc ggacgcgagc
     3001 gcgagagggg cgcccttcac gcggcatggt gccaggcgcc cctctccagc gcaccatcag
     3061 cccgaagtat tcaccagaat actttttcaa gaagacacat gcctggtgac atatgtcgcc
     3121 acaggcgtcc ggaggcagga tgtttcgggg ttgtgcccac gaacccgcgc ctcgcctggc
     3181 agtgagactt cttttcatta ttctgatgaa taatgcgggt tagcccgaag tattcaccag
     3241 aatacttcaa gaagaaggct cttgtctggt gacacatcgt tgctgccaca agcgcctaga
     3301 ggcagagtgt tttcggggct gtgcctacgg gcccgcgcta cgcctggcag ggagacttct
     3361 tttcattatt cttgatgaat aatgcgggtt agccgaggcg cacgggtcgg ccagtcctca
     3421 ccgactcttc ggcggcacag cagatctgga tcacctcaac cgcctgcagc aggtcgaacg
     3481 tctgctcacc cttctccacc atcgccagaa actctttcag catggtggag tagtaatgcg
     3541 ccgagtcggc caccttcgcg taaaagctgc cccgggtgcc ctgaaccagc ccctggaagc
     3601 cgaccggcac gccgtgcggg cacatcaccg tcagctgggc accgttgagg tacttcacct
     3661 gaacggcggt ccggccgcgc tctcccacgt cgaacacctc cactggccgg ctgttgatca
     3721 gcgtgtgccc gagggtcacc gcgtgcatcc cgtagaagag gaacgggcgt gagagcctca
     3781 ggtccgccgg gcccagagtg cacgccatga cgatctcgcc cacatccgcc tcaccgctcg
     3841 tcaccggcgc gaactccttg gtgtacttca gcgccgagga ggacatcagg aggcacttcc
     3901 gccagcgcgc caggtccatc agctcgcgcg ccgcggcgac gctgtctgcc atcggcttgt
     3961 cgatgaaagt ggggatcccc ttctcgatga agggccgggc atactgatac tgcttcatcg
     4021 tcaagtcatc gccgatgtag atcgcgtcga cgccctccaa catctcctcg aggctgctcg
     4081 cgacaacggg cacgttggcg aaccgcgaca ggttctcggc gtgttgccgg ttctcgtcga
     4141 acaccttgac cacggtggcg ccctcgatcc gcgccatcgg cggccggccg ccatacgggt
     4201 cgaggtgctt gaactcctcg ttcagcccgt tgaacatctt cgcgaactgc acggcatggt
     4261 agatgccggc accggcgaca aatccaagac ggatcatctt cttctcctct ggggcagata
     4321 gccggagtgc tgctcagcga tccaggggga tgcgcaccac cgtggcccca tcgggcggta
     4381 gctccaccgc gaagctctcg cccgaggcga gcgcgcctcg cgccgtacgc gcctctcccg
     4441 tcaggttgcc tacaaccacc agggcttcgc gcgcgccgat ataggccgcg gcagccactc
     4501 cctcctcttc caggcgcacg ggggcactcc gccagtcata gaagcgatac tggctcaggt
     4561 cgtaatcgcg cagtggcgcg aacagctcca ggtcgaggtc ctgcgccggc cagggggccg
     4621 tgccagtgag caggcagcgc agcgtcatct ggcggtggac cgattccggg gcgtcgcgct
     4681 ccaggcagcc gtagccgatg acgccgcgcg ggcgcgcacc catgaagctc cactccagcg
     4741 ggagcgaggc gagcggcggc gccgccgtcg cgagccggcc atagccccac tccatcgcga
     4801 ccacgtaatc ggccaggttc tcggtcacgg cgcacggcac catcgtgttg tggatgatga
     4861 caaggccatc ccggccgacg cgctggcgcg tccactccat cagctctacc agctcatcca
     4921 cgtcccaatg cccgaccggc gagagcgccc atccgccgta atcgggaagc tccccgggct
     4981 ccgcgtctgg ctcgtgcgcc gggttatggc agtagagggc aacgttccaa tcgtagtaga
     5041 cgccatcgag cgggtggttg cgcaggacgg tgtcgatatt ctccttgagc tgggcgagcc
     5101 agccggagcg gaggcacatc tgggcgccgt attcatcccc cgcgtaggca ttgtgaagct
     5161 gctgcccgcg ctcgccggga agccgcgccc attcggcgcc gtgcgcctgg taggccgccg
     5221 tcgtcgggtg cagctccttg ttcgagaagt aggtggccac gcgaatcccg tgctcctgga
     5281 gctcgcggat cacgcggtcg tactccttca tatcttccgg accgaagggt gggtagctcc
     5341 catcgcgcca gaagaggcca tcgcggaagg tatcgccatc gtggtggaag tgcgccgtac
     5401 gcaccccggc ctcggcccag gcgcggatct tctcgcgcgt cggccagtcg tggcggttaa
     5461 acgagcgatg gaggaagggg cggtgcgccc gcccggagag gaccgggatg cccagccagg
     5521 aatcgaacgt gtagtcgccg gccatcggca ccgcccccga ggggatgttc acgggcgaga
     5581 tggtgcacac caccgccggc gggcgcgact gtgggccgag gtagaggtgg ccataccccg
     5641 ggcgcccaca tgcctggtaa tcccactgcg acagcttcga gccagcgaac cactccaggc
     5701 cctcgcgacc ctgctccgcg cacgccacgt agcgcggcac gaaccggctc tcataggggc
     5761 agtcgaaaga ggcgcccggc gtgaaccggc cccactggca gacgccgaaa gcgcccagcc
     5821 aggcagaggc ctccgcgggt gcgccagggc gcacggcgaa cgagccgagc tccgggcgca
     5881 gcttccactg caacagcgtg agccggcgga cacgcgcgcc atcccccggg aagcggaagc
     5941 gctggcagag ccggatcgcg ccccagcggt actcgtagcg gatctgatac tcg
//
LOCUS       DAOSCV010000140         5974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8428_length_5974_cov_3.596891, whole genome shotgun sequence.
ACCESSION   DAOSCV010000140 DAOSCV010000000
VERSION     DAOSCV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8428_length_5974_cov_3.596891"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLY56_04850"
     CDS             <1..201
                     /locus_tag="PLY56_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20842.1"
                     /translation="HYFEKITEGTRVLHVIDYQFHFGALGRICGLMAVDPILRGIFNH
                     AHRATKEVCEQDWRAQESGAGR"
     gene            233..1420
                     /locus_tag="PLY56_04855"
     CDS             233..1420
                     /locus_tag="PLY56_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010585304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ20843.1"
                     /translation="MTTEHPTSPAPAQRTEGFTESVIREMTRLAHRFDALNLAQGFPD
                     FPAPEAIKEAAVRAIMADVNQYAITWGAPSLRRALADKYARHQGLEVDPEREITVCCG
                     ATEAMISALLAVINPGDEVIVFEPFYENYGPDAILSGATPRYVSLHAPDWRIDPEELA
                     SAFGPRTKAIILNTPNNPTGKVFSRAELEMIASLCTRWNALAVTDEIYEHIVYDGATH
                     LSIATLPGMRERTITISGISKTYAVTGWRIGYCIAPPRLTDAIRKVHDFLTVGAPAPL
                     QEAAAVAMGFDAAYYAELARAYQERRDVLVAALREAGFACRPPAGAYYIMADISALGF
                     SDDVQCARWLVEHAGVAAVPGSSFYHGPGKGSQQIRFAFPKRLATLEEAGRRLLKVRD
                     SLG"
     gene            complement(1518..2711)
                     /gene="nagA"
                     /locus_tag="PLY56_04860"
     CDS             complement(1518..2711)
                     /gene="nagA"
                     /locus_tag="PLY56_04860"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ20844.1"
                     /translation="MKLYGTVVGTTGVSEVTIEGEQIVSLCPASSASAPSNLVIAPGF
                     IDIQINGYGGLSFGTPEVTPEALDYVVRAQRRIGVLFICPTVCTTSHENTIHALRMLS
                     EARRDPELAHAVPCYHLEGPYIGKEDGPRGAHPLPHVRPPDWDEFRRFQDAAEGKIGI
                     ITLDPEQPGAIPFIERLVESGVIAAIGHTGATPEQIRDAVAAGARLSTHLGNGSHARI
                     DRHPNYIWEQLAADELCASIIADGHHLPPSVVKCMVRAKGVDRTILVSDAVAAAGLPP
                     GVYPSEGKEIEVLPNGRVQMKGTPYLAGSGLILSEGVGNTVRFAGVSLAEAVQMATRN
                     PASLLGLGETIGTVAPGREANLVLFRWNEQECSLDVESVVVRGRLIRVDEMEDATRPA
                     GHQAF"
     gene            2895..4163
                     /gene="glyA"
                     /locus_tag="PLY56_04865"
     CDS             2895..4163
                     /gene="glyA"
                     /locus_tag="PLY56_04865"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ20845.1"
                     /translation="MAGGSPAETAARPLSQVDPEIARAIDLEKRRQQGNIELIASENF
                     VSRAVLEAQGSILTNKYAEGYPGRRYYGGCENVDIAEELAIARARELFGAEHANVQPH
                     SGSQANMAVYLTMLQPGDTVLGMSLAHGGHLTHGSPVNFSGKLFNIVSYGLDPETEQI
                     DYDAMERLAEECRPKMIIAGASAYPRVLDFERFQAVARKVGAALMVDMAHIAGLVAAG
                     VHPSPVPYADFVTSTTHKTLRGPRAGLILCKQQYAQAIDKMVMPGMQGGPLMHIIAAK
                     AVCFREAATQEFRDYQKQIVRNARALADAFVRLGFRLVSGGTDNHLMLVDLRSKGVTG
                     KVAERVLDEVGITVNKNMIPNDPEKPLVTSGIRVGTPAVTTRGMREPEMEQIAQLIND
                     AIAGREDEAAKARVREAVRELTARFPLYTW"
     gene            4258..5274
                     /locus_tag="PLY56_04870"
     CDS             4258..5274
                     /locus_tag="PLY56_04870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ20846.1"
                     /translation="METQAQQLFMTRLLVIGAAGLAALLAYLFTPFVGELAKRVGAVD
                     RPSHRKIHERIIPRWGGVAIVVALLGAVCFAWWVASLLGVSLVSTPAAKRAFFGVLLG
                     TFMVAVAGALDDIYDLSPALQIAGQGIAATAALFLGVRIEFISNPFAADGSVLFLGDL
                     GAFITVFWIIGITKAVDLIDGMDGLAAGIAAIAAATIALMAVSAHQPQVALIAAALAG
                     GAIGFLRHNFNPAKIFMGTVGAYVLGFVLATSSIIGVLKIPVLIAMVVPILALGVPIF
                     DTSFAIVRRARERRPIFAADKGHLHHRLLNRGLSQRQTVLVLYGVTVVLCAVALAIFH
                     YTVG"
     gene            5401..>5974
                     /gene="wecB"
                     /locus_tag="PLY56_04875"
     CDS             5401..>5974
                     /gene="wecB"
                     /locus_tag="PLY56_04875"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ20847.1"
                     /translation="MSGTFKIMAVFGTRPDALKMAPVVNELKRYPEKVRVVVAVTAQH
                     REMLDQVLSIFDIRPDYDLNIMLPNQTLTQIATRALNGLDPILAAEQPQLVLVQGDTL
                     TTFAAGLAAFFHRVDVGHVEAGLRTDDKYNPFPEEMNRRLTTRLADLHFAPTERARQT
                     LLADGVRPEDIYVTGNTVIDALLATAEKPFE"
BASE COUNT         1025 a         1943 c         1939 g         1067 t
ORIGIN      
        1 cactacttcg agaagatcac cgagggcacg cgcgttctgc acgtcattga ttaccagttc
       61 catttcggcg ctctcgggcg gatctgcggt ctgatggcgg ttgatcccat cctgcgcggc
      121 attttcaacc acgctcaccg cgccacgaaa gaggtctgcg agcaagactg gcgggcacag
      181 gaatcgggcg ccgggcgttg acttgcctgc ccccggctcg tataatgggg gcatgacaac
      241 cgagcatccc acctcacccg cgccggcaca gcgcaccgag ggcttcaccg agtcggtaat
      301 ccgcgagatg acgcgcctag cgcatcgctt cgacgccctc aacctggccc agggctttcc
      361 ggacttcccg gcgccggagg cgatcaagga agccgccgta cgcgccatca tggccgacgt
      421 caaccagtat gccatcacct ggggcgcgcc ctcgctgcgt cgcgcgctcg ccgacaagta
      481 cgcgcgccac cagggcctgg aggttgatcc cgagcgcgag attaccgtct gctgcggcgc
      541 caccgaagcg atgatctccg cgctcctcgc cgtgatcaac ccgggcgacg aggtgatcgt
      601 cttcgagccg ttctacgaga actacgggcc agatgcgatc ctctccgggg cgactccccg
      661 ttacgtctcg ctgcacgcgc ccgactggcg catcgatccg gaggagctgg cgtccgcgtt
      721 tggcccgaga accaaagcga tcatcctcaa cacgcccaat aaccccacgg gaaaggtctt
      781 ctctcgcgcc gagctcgaga tgatcgcgag tctgtgcacg cgctggaatg ccctggcggt
      841 gaccgacgag atttacgagc acatcgtcta cgacggagcg acgcatctca gcatcgccac
      901 gctgccgggg atgcgcgagc gcaccatcac catctccggg atctcgaaga cctacgcggt
      961 caccggctgg cgcatcggct actgcatcgc gccgccacga ctgacggatg ccatccggaa
     1021 ggtgcatgac ttcctcaccg tgggagcgcc ggccccgctg caggaggccg cggcagtcgc
     1081 catgggcttc gatgccgcgt actacgctga actggcgcgc gcctaccagg agcgccgcga
     1141 cgtgctggtg gctgccctcc gcgaggcggg ttttgcctgc cgcccgcccg ccggcgccta
     1201 ctacatcatg gccgatatct cagcccttgg cttctcagac gatgtgcagt gcgcgcgctg
     1261 gcttgtggag cacgcaggcg tcgcagccgt acctgggagc agcttctacc atgggccggg
     1321 gaaggggagc cagcagattc gtttcgcttt cccgaagcgc ctggccacac tggaggaggc
     1381 gggacggcgg ctgctcaagg ttcgcgacag cctgggctga gaagatagga gacaccctcc
     1441 cgtcgcgtgc gagggtgtct ctgcgcatca atcatatctc ccggaaggca tcgccccctg
     1501 ggagtgatag gacgtgctca gaaggcctgg tggcctgcgg ggcgtgtggc gtcctccatc
     1561 tcatcgaccc gaatcaggcg cccacgcacg acgacgcttt ccacgtccag cgagcactcc
     1621 tgctcgttcc accggaagag caccaggttt gcttcgcggc ccggcgccac cgtgccgatg
     1681 gtctctccca gccccagcaa ggaagcgggg ttgcgagtcg ccatctgcac ggcttccgcc
     1741 agcgagacgc ccgcgaagcg cacggtgttc ccaacgccct cggagaggat caacccggag
     1801 cccgcgagat agggtgtacc cttcatctgc acacgcccgt tgggaagcac ctcgatctcc
     1861 ttgccctcgg agggatagac gccagggggc aggccggcgg cagcgacggc gtcgctcacc
     1921 aggatcgtgc gatccacgcc cttagcgcgc accatgcact tcactacgga aggcggaagg
     1981 tggtggccat cggcgatgat gctggcgcac agctcatcgg ccgccagctg ttcccagatg
     2041 tagttcgggt ggcggtcgat gcgcgcatgc gagccgttgc cgaggtgtgt cgagaggcgg
     2101 gcgccagcgg cgacggcatc gcggatctgc tcgggcgtgg cgccagtgtg cccgattgcc
     2161 gcaatcacgc ccgattcaac caggcgctcg atgaagggga tcgcgcccgg ctgctcgggg
     2221 tccaaggtga tgatgcctat cttgccttcg gcggcgtcct ggaagcgccg gaactcatcc
     2281 caatcgggcg gacgcacatg tggcagcgga tgcgcgcctc gcgggccatc ttccttgccg
     2341 atgtacgggc cttccaggtg gtagcagggc accgcgtgcg ccagctccgg gtcgcgtcgc
     2401 gcctcgctca acatgcgcaa ggcgtgaatg gtgttctcgt gcgacgtggt acagaccgtc
     2461 gggcagatga agagaacgcc aatccgtcgc tgtgcgcgga ccacatagtc aagtgcttcg
     2521 ggcgtgactt cgggagtgcc gaagctgagc ccgccatagc cgttgatctg aatatcaatg
     2581 aaccctggag cgatcaccag gttggacggc gcgctcgctg aggaagcggg gcatagcgag
     2641 acaatctgct cgccctcaat ggtgacttcc gagacaccag tagtaccgac aaccgttccg
     2701 taaagcttca cggccaatct cctccgttcg ccgccagtat agcacagggt cccgagtaag
     2761 tcaacgcgac gcccggcgtg cgtcatcgga ggggaatagg gggcgacggc ggcttcgatt
     2821 catgatataa taggggcggc tatcatgagt cacggccagg agagggaaca gaacggacca
     2881 tggacctgaa ggaaatggcg ggtggctctc ccgcggagac tgccgcccgg ccgctgtcgc
     2941 aggtggatcc agagatcgcg cgcgcgattg acctggagaa gcggcggcag cagggtaaca
     3001 tcgaactcat cgcctcggag aacttcgtct cgcgtgccgt cctcgaagcg caaggcagca
     3061 tcctgacgaa caagtatgcc gagggctatc ccggacgccg ctactacggc ggctgcgaga
     3121 acgtcgatat cgccgaggag ctcgccattg cgcgtgcccg agagcttttt ggcgccgagc
     3181 acgcgaatgt gcagccccac tccgggtcgc aggcaaacat ggccgtttac ttgacgatgc
     3241 ttcagccggg cgataccgtt ttggggatga gcctggcgca tggggggcac cttacccacg
     3301 gcagcccggt gaacttctcc ggcaagctct ttaatatcgt ttcttacgga cttgatcccg
     3361 agacggagca aatcgactac gacgcgatgg agcgcctcgc agaggagtgc cgtccgaaga
     3421 tgatcatcgc cggcgccagt gcctacccgc gcgtcctcga tttcgagcgg ttccaggcgg
     3481 tcgcccggaa ggtgggcgcc gcgctcatgg tcgatatggc gcatatcgcg ggcctggtcg
     3541 ctgcgggcgt acacccctcg ccggtgccct acgccgattt tgtcaccagc accacgcaca
     3601 agacgttgcg cggcccccga gcaggcctca tcctctgcaa gcagcagtac gcccaggcga
     3661 ttgataagat ggtgatgccg gggatgcagg gcggaccgtt gatgcacatc atcgccgcca
     3721 aggccgtctg cttccgtgaa gccgccaccc aggagtttcg cgactaccaa aaacagattg
     3781 ttcggaatgc ccgcgccctc gcggacgcgt tcgtgcgcct cggcttccgc ctggtttccg
     3841 gtggcacaga caaccacctg atgctggtcg acctgcgctc gaagggggtg accggcaagg
     3901 tcgccgagcg cgtgctcgac gaggtcggca tcaccgtgaa caagaatatg atccctaacg
     3961 acccggagaa gccgttggtg acttccggta tccgggtggg cacgcccgcc gtgacgacgc
     4021 gcggcatgcg cgagccggag atggagcaga tcgcgcagct gatcaacgac gcgattgccg
     4081 ggcgtgaaga tgaagcggcg aaggcgagag tgcgcgaggc cgttcgggag ttgaccgcgc
     4141 gctttccgct gtatacctgg tagcggggag tgggggagtg ttggagccgc gagcgccatc
     4201 gcgccagcac tccccctgtt cccccagtga caacgtccga ggggctagaa ccagggcatg
     4261 gagacacaag cacagcagct gtttatgacc cgcctgctgg tgattggggc cgccgggctc
     4321 gcggcactcc tggcgtacct gttcaccccc ttcgtgggag agctggccaa gcgcgtgggc
     4381 gccgtagacc ggcctagcca ccggaagatc catgagcgga tcatcccccg gtggggcgga
     4441 gtcgccatcg tcgtcgcgct cctcggagcc gtctgcttcg cctggtgggt cgcctcgctc
     4501 ctcggggtct ccctagtctc cacacccgcg gccaaacgcg ccttcttcgg cgttctcctc
     4561 ggcacgttca tggtggcggt ggccggggcg ctggacgaca tctacgatct ctctcccgcc
     4621 ctccagatcg ccggccaggg gattgccgcc acggcggcgc tcttcctggg cgtgcgcatc
     4681 gagttcatct ccaacccctt tgccgcagat gggagcgttc tcttcctggg cgacctgggg
     4741 gcgtttatta ccgtcttctg gatcatcggg atcaccaagg cggtggatct gatcgatggc
     4801 atggatgggc tggcggccgg gatcgcggcg attgccgccg cgacgattgc cctgatggcg
     4861 gtctccgcgc atcagccgca ggtggcgctc attgcggccg cgcttgccgg gggcgctatc
     4921 ggtttcctgc gccacaactt caatccggcg aagatcttca tggggacggt gggtgcctac
     4981 gtgcttggat tcgtgctggc aacatcctcg atcatcggcg tgctcaagat accggtgctc
     5041 atcgccatgg tagttccgat cctggctctg ggggtgccca tcttcgatac ctcatttgcc
     5101 attgtgcgcc gcgcgcgcga gcgccgtccg atctttgccg cggataaggg ccacctgcac
     5161 catcgcctgc tcaaccgcgg gctctcgcag cggcagaccg tcctggtgct ttacggagtc
     5221 accgtcgtgc tctgcgcggt ggcgctggcg atcttccact atacggtggg gtgagacgct
     5281 ggggagcgcg tcgcgtgggc cagtgcagcg agcgtccgtg ataaacccgg tttcctggcc
     5341 gggctccgtg gcagcccagg atgcccttgc ttccttcact cgataggggg tctaattcta
     5401 atgagcggta ccttcaagat catggccgtg tttggcaccc gtccggatgc gctgaagatg
     5461 gcgccggtcg tcaacgagct gaagcgctat ccggagaagg tgcgcgtggt ggtggccgtc
     5521 accgcgcaac accgggagat gctggatcag gtcctctcca tcttcgatat tcgtcccgat
     5581 tatgacctga acatcatgct cccgaaccag acgctgacgc agattgccac gcgagcgctg
     5641 aacggcctgg atccgatctt ggccgcggag cagccccagc ttgtcttggt gcagggcgat
     5701 acgttgacca cttttgccgc cggcctggct gcctttttcc accgcgtgga tgtgggccac
     5761 gtcgaggcgg gcctgcgcac cgacgacaag tacaatccct tccccgagga gatgaaccgc
     5821 cggctgacga cgcgactggc cgacctccac ttcgcgccca cggagcgcgc ccgacagacg
     5881 ctgctggccg atggcgtgcg ccccgaggat atctacgtga ccgggaatac ggtcatcgac
     5941 gcgctgttgg ccaccgcgga gaagccgttc gagt
//
LOCUS       DAOSCV010000141         5949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8478_length_5949_cov_3.362233, whole genome shotgun sequence.
ACCESSION   DAOSCV010000141 DAOSCV010000000
VERSION     DAOSCV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8478_length_5949_cov_3.362233"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLY56_04880"
     CDS             <1..191
                     /locus_tag="PLY56_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOJ20848.1"
                     /translation="EMSQGIAQLLNDIHIRGTTVVVASHDRAVVNALRKRVITLERGR
                     VVRDEVGGGYSPDEFQTP"
     gene            172..1059
                     /gene="ftsX"
                     /locus_tag="PLY56_04885"
     CDS             172..1059
                     /gene="ftsX"
                     /locus_tag="PLY56_04885"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ20849.1"
                     /translation="MNFRHLEFLLHETLTGIRRNALMSLAAISTIAVSLFLLGSLRLA
                     IVNLEEMAGALCGRFEMRVFLKMHLAADKRDDLGKQIAAMPGVKECQLVRKEEAWPEL
                     CHRLQGTVEISDLGGVNPLPDAFTVHVEDLEQMGALADRIRVLPGVDEVTDTRVAAQR
                     MAAIVRAIRLGGTIAVAILAVVAMVIVNNTIRLTVHARRREINIMQLVGATNACVRTP
                     FLLEGAFHGMAGALGALLLLAVGYSYLHEEVRNVVPFLELIPPSAELLWAQGPALLGA
                     GVGLGLLASLLAVRRYLHG"
     gene            1073..2239
                     /locus_tag="PLY56_04890"
     CDS             1073..2239
                     /locus_tag="PLY56_04890"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ20850.1"
                     /translation="MSRRTRRGWAILCAVVWAIASLGGVTHAAKPKRVPATKRATQRK
                     LASLRQEIRSTQQRLRQTKHRQRKVSEELRESQENLRNARERLRQTRAQLAYAERDLA
                     DAQRRLDIAQKRLATHVSQLRGRLVDIYKYGTASYAVVLLQSNDMWDLTSRGYMLQRI
                     VHHDVDLLNRIKQEKAAIALQTRRLAARRDEVARLRVSHERQIVAVTDCLQTQREIQE
                     ALRRDRARLEAALAELEENSREVERMLKAFEARSDIARRIPKPWLGRFARPVGGRITS
                     HFGTRFHPILRVTKLHTGVDIAAPSGTPIAAVGDGVVVHAGWWGGYGNCVVIAHGNGR
                     STLYAHCSSLAVPRGARVRKGQIIGRVGSTGFSTGPHLHFEVRMNGRPVNPMSH"
     gene            2469..3857
                     /locus_tag="PLY56_04895"
     CDS             2469..3857
                     /locus_tag="PLY56_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ20851.1"
                     /translation="MRRAAWMLVLVGFVAGAFTAGGSAYRLVQRNRQTRSLEMYGSVL
                     RIAGRSPLALPAGNLAKQTDLRPLDLFSNVERRLRANYYEKIENDTVLAFGTADNMLD
                     SLGDPYSRYLEPDQLKAYAERQRGIYRGIGAVLDVRKEQPPANQSGKPEQGAPEEDED
                     SWPAYDDPVDLNSVARSPEGTPLYRYEVFVVATVPGGPAEAAGLLPGDVIRKVNGKFI
                     FTKNLDTLERMLLMPVEGSVRLAIQREGTAKPIDLSVPMRETRVDLVTSRVVDRVGVI
                     TIRALGLGAADGVKAKLREMAASRVSGVVLDLRRNAEGTFEEACAVASLFVPQGPVAW
                     LKEQGGKMRAVNASAPNGRPEERRLAVLIDDTTAGPAELVAAAVKGRGVGRLLGVTTR
                     GRAGRQELFRMPGDSAILLTTGLFFGPTAQAPLLIEGKGVVPDVAVTARGSAQDQALQ
                     RAIAWIKSGGAA"
     gene            3858..5249
                     /locus_tag="PLY56_04900"
     CDS             3858..5249
                     /locus_tag="PLY56_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ20852.1"
                     /translation="MKENRFRHTIFGFAITGLLAFLVGFLYHVVAFASPTPLPVHISQ
                     VLGGTSGGGAAEADDASVKPLEVFWKTYTLVRQRYRLVGVPGWDDDNLIYAGLDGLLR
                     SLGDPYTRFLDPKAYKQMIEQNQGQFQGIGAYLQQSRDQRHILLAPIPNGPAAAAGLK
                     RGDILLKINGTNAVGMDIDKAKNLIRGPAGTTVELTVGRRQDPSRPEGADNPMKTVKI
                     VVERAVIKPDEVKFSAGPPAGHEKETEIGYIRLHQFNEDTENQLDRALVQLGKKRVKG
                     IVLDLRGNPGGLLDVAIGVASRFLEEGPVVFIQEAGGNRRAYNANRRQFNGEHRKYLP
                     ANTPVVVLVNRDSASASEIVAGCLKDHKIAKLVGETTFGKGLVQTIIPLEADGNRGAL
                     KITTAKYFTPNGTDINHKGIEPDYKVVARGPDEDPFAAETTKEDPQLDRAVEVIRKQI
                     QGGAAALAEGAKH"
     gene            5472..>5949
                     /locus_tag="PLY56_04905"
     CDS             5472..>5949
                     /locus_tag="PLY56_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ20853.1"
                     /translation="MESVLDVIRHGGVAMYPLLLCSVLAVAVSLERLLAIRRARVDAG
                     GLIEQLREPLARGDLEEARRICQSAPVPLAAVALAGLAKLGRPRQEMEDAMVHASNLA
                     MDDLESNLPVLGTIGGITPFIGLFGTVLGIMRAFRDIQARGQAGTAVVAGGVSEAQV"
BASE COUNT         1036 a         1753 c         2123 g         1037 t
ORIGIN      
        1 cggagatgtc gcaagggatc gctcagctac tgaatgatat ccatatccgc gggaccacgg
       61 tggtggtggc gagccatgac cgggcagtgg tgaatgccct gcgcaagcgc gtcatcactt
      121 tggagcgtgg ccgagtcgtc cgggatgagg tcggaggggg ctatagcccg gatgaatttc
      181 agacaccttg aattcctgct gcatgagacg ctgaccggca tccgccggaa tgcgttgatg
      241 agcctggccg cgatcagcac gatagccgtc tcgctttttt tgcttggcag cctgcgcctg
      301 gccattgtca acctggagga gatggcgggc gcgttgtgcg ggcgttttga gatgcgggtc
      361 ttcctcaaga tgcacctggc ggcggacaag cgcgatgatc tgggcaagca aatcgctgcg
      421 atgcccggcg tgaaggagtg ccagctggtg cgcaaggagg aggcgtggcc ggagctgtgc
      481 catcgtctgc aagggacggt ggagatcagt gacctggggg gcgtcaaccc cctgcctgat
      541 gcgttcaccg tgcatgtgga ggatctggag cagatgggtg cgctggccga ccggatccgc
      601 gttttgcccg gggtggacga agtgacggac acgcgtgtgg cggcccagcg catggcggcc
      661 attgtgcgtg cgatacgcct gggtgggacc attgcggtgg ctattctggc cgtggtggcg
      721 atggtcatcg tgaacaacac catccgcctc acggtccacg cgcgccgcag ggagatcaat
      781 atcatgcagc tcgtgggcgc gacgaacgcc tgcgtgcgca cacccttcct attggagggg
      841 gctttccacg gaatggcggg ggcgttgggg gccttgctcc tcctggcggt gggctattcc
      901 tacttgcatg aggaggtgcg caacgtcgtg cccttcctgg agctgatccc accatcagcg
      961 gagttgcttt gggcgcaggg acccgcgctg ctcggagccg gggtgggcct ggggctgttg
     1021 gctagccttc tagccgtgcg ccgatatctg catgggtgag gatgaagttt tgttgtctcg
     1081 taggacacgc cgcgggtggg caatcctctg cgcggtggta tgggcgattg cctcgctggg
     1141 gggggtgacc cacgcggcga agccgaagcg cgtgccggcg accaagaggg ccacgcagcg
     1201 caagctggca agcctgcgcc aggagatccg gagcacgcaa cagcgcctgc gccagacgaa
     1261 gcaccgccag cgtaaggttt ccgaggagtt gcgcgaaagc caggagaacc tccggaatgc
     1321 gcgcgagcgc ctgcgccaga cgcgcgcgca gcttgcttac gcggagcgag atctggccga
     1381 cgcgcagcgg cgcctcgaca tcgcccagaa gcggctggcg acgcatgtca gccaacttcg
     1441 cggtcgcctg gtcgatatct acaagtacgg cacggcaagc tacgcggtcg tcttgctcca
     1501 gtccaacgac atgtgggatc tgaccagccg tggctacatg cttcagcgca ttgtccatca
     1561 cgacgttgac ctgctgaacc ggatcaagca ggagaaggcc gcaatcgcgc tccagacgcg
     1621 ccgcctggca gcccggcggg atgaagtggc ccggctgcgc gtctcgcatg agcggcagat
     1681 cgtggcggta accgattgcc tgcaaactca gcgcgagatt caagaggcgc tgaggcgcga
     1741 ccgggcgagg ctggaagcgg ccctggccga gttggaggag aactcgcgcg aggtggagcg
     1801 gatgctgaag gcgtttgagg cccgctcgga tatcgcccgg cggattccaa agccctggct
     1861 cgggcggttt gcccgaccgg tgggggggcg gatcacctcg cacttcggaa cgcgctttca
     1921 tccgatcctg agagtgacca agctccacac gggcgtcgat atcgcggcgc cttccggcac
     1981 gccgattgca gcggttgggg atggcgtggt cgttcatgcg gggtggtggg gtggctatgg
     2041 caactgcgtg gtgatcgccc acggcaacgg gcggtccacg ctctatgcgc actgctcctc
     2101 gctggcggta cctcgcgggg cgcgtgtccg aaaggggcag attattggca gggttggcag
     2161 caccggattt agcaccggtc cgcacctcca tttcgaggtg cgcatgaacg gccgaccggt
     2221 gaatccgatg tcgcactgac cgcggcgttc gataaatcgt ttgggcgcat ggaactctaa
     2281 tcgcctgggg agcgtcctca ccaggggaag agggattctc tgggccgcat cggcttctgg
     2341 cgaaggcgct cggagtgata ccagcgttta gagccggggc ttctgcggaa gactactcag
     2401 ggggcctgcc agcaaaggtt tcaggtacaa ctgaagggcg aaatggggat gcctgtatga
     2461 ggatcccgat gaggcgtgcg gcatggatgt tggtgctggt gggtttcgtg gccggcgcct
     2521 tcacagcggg gggaagcgcc taccggttgg ttcaacgcaa ccggcagacg cgcagcctgg
     2581 aaatgtacgg atcggtgctt cggatcgcgg ggcgctcgcc actcgcgctg cctgcgggaa
     2641 acctggcgaa gcagaccgat ctccggcccc tggatctctt ctccaacgta gagcgccgcc
     2701 tgcgcgccaa ctactacgag aagattgaga atgacacggt gctggccttc gggacggccg
     2761 ataacatgct cgactcgctt ggcgatccct actcgcgtta cctcgagccg gatcagctga
     2821 aggcctacgc ggagcgccag cggggcatct accgcgggat cggagcggtg ctcgatgtgc
     2881 gcaaggagca gccgcccgcg aatcagagcg gaaagccgga gcaaggcgcg cccgaggaag
     2941 acgaggactc ctggccagcg tatgacgacc ccgtggatct caactccgta gcgcgttccc
     3001 cagagggcac gccgctctac cggtatgaag tcttcgtcgt tgcgactgtg ccgggcgggc
     3061 ctgcggaggc cgctggcttg ctcccgggcg acgtgatccg caaggtcaac ggcaagttca
     3121 tcttcaccaa gaacctcgac acgctggagc gcatgttgct gatgcccgtg gagggctcgg
     3181 tgcgcctggc gatccagcgc gagggcaccg ccaagccgat tgatctatcg gtgccgatgc
     3241 gcgagacgcg cgttgacctg gtgacgagcc gggtggtgga tcgcgtcggc gtgatcacca
     3301 tccgagcctt ggggctgggt gccgccgatg gggtgaaggc gaaactccga gagatggccg
     3361 cttcacgcgt cagcggggtg gtgctggatc tgcgtcgcaa cgccgagggt acctttgagg
     3421 aagcgtgcgc ggtcgccagc ctctttgtgc cacagggacc ggtggcgtgg ctgaaggagc
     3481 agggcggcaa gatgcgcgcg gtcaacgcga gtgccccgaa tgggcggccc gaggagcgcc
     3541 gactcgcggt gctcatcgat gatacgacgg cgggcccagc cgagctggta gccgctgccg
     3601 tgaagggccg aggcgtcggc cgactcctgg gcgttacgac gcggggccgg gccggacggc
     3661 aggagctgtt ccggatgccg ggcgactcgg cgattctgct gacgacgggg cttttcttcg
     3721 ggcccacggc acaggctccg ctgctcatcg agggcaaggg cgtggtgcct gacgtggcgg
     3781 tgaccgcgcg cgggagcgcc caggatcagg cgctgcagcg cgcgattgcc tggatcaaga
     3841 gcggaggggc agcctagatg aaagagaatc gcttccgcca cactatcttc gggtttgcga
     3901 tcacggggct gctcgccttc ctggtcggct tcctctatca cgtggtggcg ttcgcgtctc
     3961 caacgccgct tccagtccac atctcccagg ttctgggggg aacgtctggt ggtggcgccg
     4021 ccgaggcaga cgacgcttcg gtgaagccgc tggaggtctt ctggaagacc tatacgttgg
     4081 tgcggcagcg ctaccggctg gtcggcgtgc ccggatggga cgacgacaat ctgatctacg
     4141 ccggcctcga tggcctcctc cggtcgctgg gcgatcccta cacgcgcttc ctggatccca
     4201 aggcgtataa gcagatgatc gagcagaacc aggggcagtt ccaggggatc ggcgcctatc
     4261 tgcagcaatc gcgagaccag cggcacatcc tgctggcgcc cattcccaac gggcccgctg
     4321 ccgcagccgg actcaagcgc ggcgatattc tgctcaagat caacggcacg aacgccgttg
     4381 gcatggatat cgataaggcg aaaaacctca tccgtggccc cgccggaacg accgtggagc
     4441 tgaccgttgg ccgccgccag gatccctcgc gccccgaggg ggccgacaat ccgatgaaga
     4501 cggtgaagat cgtggtggag cgcgcggtca tcaaaccgga tgaggtgaag ttcagcgccg
     4561 gacctcccgc cgggcacgag aaggaaaccg agatcggtta tatccggctt caccagttca
     4621 acgaggatac cgagaatcag ctcgaccggg cgctcgtcca gctgggcaag aagcgggtaa
     4681 agggaatcgt gctcgacctg cgcggcaatc cgggcgggct cctggatgtg gccatcggcg
     4741 tggccagccg gttcctggag gaaggcccgg ttgtcttcat ccaggaagcg ggcgggaatc
     4801 ggcgcgccta caacgccaac cggcgccagt tcaacggcga gcatcgcaag tacctgccgg
     4861 ccaatacgcc ggtcgttgtc ctggtgaacc gcgacagcgc ctcggcctcg gagatcgtcg
     4921 ccggctgtct gaaggaccac aagattgcca agctggtggg cgagaccacc tttggcaagg
     4981 gcctggtgca gacgatcatt ccgctcgaag ccgatggaaa tcgtggcgcg ctcaagatca
     5041 cgaccgccaa gtacttcact ccgaacggca cggacatcaa ccacaagggg atcgagccgg
     5101 attacaaggt ggtggcgcga ggccccgacg aggatccgtt cgctgcggag acgaccaagg
     5161 aagacccgca gttggaccgt gccgtggagg tgatccgcaa gcagatccag gggggcgcgg
     5221 cggccctcgc cgagggggct aagcactaac ccattcgcag agcgcgtggg aagacggccg
     5281 cttcggccga tgcgccttgg atcacgcgca ggatggttac tctatcggcc cagcggctgc
     5341 ccaatcgagg agcagtcgct gggccgtggc attagtggaa cctttcgtgt tgggaacgca
     5401 cctaataata gggcgggcag ggtttgtttc ggcattggtt gtgcctgttg cccctggggg
     5461 gagtgcgaga gttggagagc gttcttgatg tgatacgcca cggcggcgtg gcgatgtatc
     5521 ccttattgct ctgttctgtg cttgcggttg ccgtctcgct cgagcggcta ctggcgattc
     5581 ggcgggcgcg cgtggatgct ggcggcctga tcgagcagct gcgcgagccg cttgcgcgcg
     5641 gcgatctgga agaggcgcgg cggatctgcc agagcgcgcc tgtgccgctc gccgccgtgg
     5701 cgcttgccgg cctggcaaag ctgggccgcc ctcgccagga gatggaggat gcgatggtgc
     5761 acgcctccaa cctggcgatg gatgatctgg agagcaacct cccggtgctc ggcacgatcg
     5821 ggggaatcac tcctttcatc ggcctcttcg gcaccgtctt ggggatcatg cgcgcgttcc
     5881 gtgacattca ggcgcgcgga caggcgggca ccgccgtggt ggcaggcgga gtcagcgagg
     5941 cgcaggtgg
//
LOCUS       DAOSCV010000142         5946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8487_length_5946_cov_3.474792, whole genome shotgun sequence.
ACCESSION   DAOSCV010000142 DAOSCV010000000
VERSION     DAOSCV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8487_length_5946_cov_3.474792"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /gene="thrS"
                     /locus_tag="PLY56_04910"
     CDS             complement(<1..1079)
                     /gene="thrS"
                     /locus_tag="PLY56_04910"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ20854.1"
                     /translation="MEQESLERLRHSTAHVMAQAVKELFPEARLAIGPPIQDGFYYDF
                     DVPRPFTPDDLERIEARMREIIAADHPITRTEQSREEAIRYFEERGEVYKVELIREIP
                     EETVSFYSQDNFVDLCRGPHVDSTGEIKAFKLLSVAGAYWRGNEKNPMLQRIYGTAWP
                     TQEELDAYLANLAERERRDHRRLGKELDLFSQHEEVGAGLIFWHPKGAMIRHLIESFW
                     KEEHIKRGYQFVYTPHIASEELFRISGHLEKYSESMYAPIDIEGQPYRLKAMNCPGHI
                     MIYKTHLRSYRDLPIRYAELGTVYRFERSGTLTGMLRVRGFTQDDAHIFCTPEQLVGE
                     VIGVLDFVDYVMARFGYTYKAYLATR"
     gene            complement(1563..2867)
                     /locus_tag="PLY56_04915"
     CDS             complement(1563..2867)
                     /locus_tag="PLY56_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20855.1"
                     /translation="MLQVGSASVDITPNLGCHLIGYFNDRIATAIHDPLHAKALAISN
                     GEMTIGIVVCDLIAVPGDVVRAAKERIASETGVPPERVMICATHTHTGPSIKSSLGTP
                     ATEGYAEWVAPRIADAFIQASRQLRPAQVAFTRGNCASEVHNRRRVMKDGSVRMNPGY
                     QHPDMVRPAGPTDPELGVLIFRSPDREPLGVLANLGLHYVGAGVHDAVSADYFAEFGV
                     ALQRIAGARFQAILANGCFGDINNCDFDRPARTSPDPYFQIRRVANVVAAEAWKAWNI
                     LREEDFRDEVPLGAQLEMVPFKARVPSPEELAQAKARLAAGDDPNDHEWVYARELVLM
                     ESEPSEWEVPISAMRIGELGLVGLPGEVFSQIGLDIKAASPFAQTMLLGLANGTVGYV
                     ATDQAMDEGSYETRLCRHVRAPKGTHALWMETAVRLLNGLKG"
     gene            3174..3249
                     /locus_tag="PLY56_04920"
     tRNA            3174..3249
                     /locus_tag="PLY56_04920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3207..3209,aa:Ala,seq:ggc)
     gene            3299..3580
                     /locus_tag="PLY56_04925"
     CDS             3299..3580
                     /locus_tag="PLY56_04925"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOJ20856.1"
                     /translation="MEPHADSTEEFVVVMEPRDSVAASLAKGILENEGIPVLVESYQI
                     PWYDGIMTPATGAWGRILVPARLASNAKAVLSAYFAEQEDEAAPTTDPE"
     gene            complement(3585..5417)
                     /locus_tag="PLY56_04930"
     CDS             complement(3585..5417)
                     /locus_tag="PLY56_04930"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20857.1"
                     /translation="MSDSRRREKHSPVPCWNAEVDLAGRDAPTDPPAVASGDWLLLLP
                     LGAGAVGLDLGCGDGALSLELAHYCDQVVAVDPDPANVQIARRRASAAGLSHLHVCRG
                     NLDTLISARRRFDLVVVRDPRRVLAGLRPAQAVERLAELVAPQGCLAIAAGKPPEGVT
                     PLQPGTRARGARSPWWGEYGARRLHRMLEAVGFPNPQILAAVPDPHQPEILFPVGGEG
                     QGRVAGCLISRCLRPGTASRSTLLYATTALSQWRAARWICPGFWVIARAPACMRATMA
                     EEMVRRIVARPRSGEWRRGSATERARVALRARDLNFVLSRPHAGEGDHAALLVVPAGW
                     GHPACILRAGRWRGEAPPVGDGQAGLAELRSRVVGRLRDSLPEPLGASEVREHHAAAH
                     AYIPGVPLHQKVVAASPRQARAVAEHALQAVATWLAELHEQTRASAWLEGEAVLEKVG
                     PLLVPAAGAASGGPLVKRVASGIAEAGLWLGAAHNHLRPCHILLQEDGIAVTGWEHLA
                     LEGLPLMDLLGFAHALGELLAGGGAMDPGVAWRFAYREENWYSRAVRMAMEDYCERVS
                     IDRAAVPFYLATTYLRAAARQDPFGKAGPLIHLAGLSLDTLDWR"
BASE COUNT         1046 a         2007 c         1941 g          952 t
ORIGIN      
        1 cgggtcgcca ggtaggcctt gtatgtgtag ccgaagcggg ccatgacgta gtccacgaag
       61 tccaggacgc caatcacctc gcccaccagc tgctcgggcg tgcagaagat gtgcgcgtca
      121 tcctgggtga agccgcgcac gcgaagcatc ccggtgaggg tgcccgagcg ctcgaagcgg
      181 tacaccgtgc ccagctccgc atagcgtatc ggcaggtcgc gataggagcg gaggtgagtc
      241 ttgtagatca tgatatgccc agggcaattc atcgccttca gccggtaggg ctggccctcg
      301 atatcaatgg gggcatacat gctctcggag tacttctcca ggtggccact gatccggaac
      361 agctcctcgc tggcgatgtg cggcgtgtag acgaactggt agccacgctt gatgtgctct
      421 tccttccaga agctctcgat caggtggcgg atcatcgcgc ccttgggatg ccagaagatg
      481 agcccggcgc ccacctcctc atgctggctg aagagatcca gctccttgcc caggcgacga
      541 tgatcccggc gctcgcgctc cgccaggttt gccagatagg catccagctc ttcctgcgtc
      601 ggccaggcgg tgccgtagat gcgctggagc atcgggttct tctcgttgcc acgccagtag
      661 gccccggcca ccgagaggag cttgaacgcc ttgatctcgc cggtgctatc cacatgcggc
      721 ccgcggcaga ggtcaacgaa gttgtcctgg ctgtagaagc tgaccgtctc ttctgggatc
      781 tcccggatca gctccacctt atagacctcg ccgcgctcct cgaagtagcg gatcgcctct
      841 tcgcgcgact gctcggtccg cgtgatcgga tggtcggccg cgatgatctc gcgcatgcgg
      901 gcttcaatcc gctccagatc atccggcgtg aagggacggg ggacatcaaa atcgtagtag
      961 aagccatcct ggattggggg gccgatggcc agtcgggctt cggggaagag ctccttcacg
     1021 gcctgggcca tgacatgcgc cgtggaatgc cgcaagcgct ccagggattc ctgttccatg
     1081 tgattatcct ccgtgccggc gcgatacaga tgcgccgccg gctaaaatga gggggccctc
     1141 accggcccgc gagacggacg cgcttacggg gcacatcact cctcgcgcac ctgagaagac
     1201 ccacacgcca acaaggggtt gaaacgacag cctcggatag caggcgccga ccgtttgcac
     1261 cgccagcacc tggctccgtg ccacttctcc ccggctctat tgtagtattc ggcagacggg
     1321 agagtcaagg aaagagatgg gattcctggg attccgacat atggagaggg ccgccctcac
     1381 gcgcagtgct tcgagcaccg cgcgtggggg ggcgcgcgat gcagggggaa ccgagttgcg
     1441 cccacgcgtt gcccgggccg gcagcgatcc cgcggctgga gggaacagac gcgggtggga
     1501 tagaggtccc gaaggggccg gcagcatgcg cccccttcgg gatcgaggca tggcgcaggc
     1561 agttagccct tcaggccgtt gagcaggcga acagcggtct ccatccagag ggcatgcgtg
     1621 cccttaggag cgcgcacatg gcggcagagg cgcgtctcgt agctcccctc gtccatcgcc
     1681 tggtccgtcg ccacatagcc cacggtgccg ttggccaacc cgaggagcat cgtctgcgcg
     1741 aaaggcgacg ccgccttgat atcgaggccg atctggctga agacctcgcc cggcaggccc
     1801 accaagccca gctcgccgat ccgcatcgcg ctgatcggca cttcccactc ggagggctcg
     1861 gattccatca ggaccagctc gcgggcgtaa acccactcgt ggtcgttggg atcgtcaccg
     1921 gccgcgaggc gcgccttggc ctgcgccagc tcctccggcg agggcacgcg cgccttgaag
     1981 ggaaccatct ccagctgggc gcccaggggc acctcgtcgc ggaaatcctc ctcgcggagg
     2041 atgttccacg ccttccacgc ctcggccgcc accacgttag ccacgcgccg gatctggaag
     2101 taggggtcgg ggctggtgcg cgccggccta tcgaagtcac agttgttgat gtcgccgaag
     2161 cagccgttcg cgagaatcgc ctggaagcgc gcgcccgcga ttcgctgcag cgccacgccg
     2221 aactcggcga agtagtcggc gctgacggca tcatgcacgc cggcgccgac gtagtggagc
     2281 cccagattgg cgagaacgcc gagcggctcg cgatccggcg accggaagat gaggacgccc
     2341 agctccgggt cggtcggtcc ggcggggcgc accatatccg ggtgctggta gccgggattc
     2401 atgcgcaccg agccgtcctt catgacacgc cgccggttgt gcacttcgga ggcgcagttg
     2461 ccccgggtga aggcgacctg cgccgggcga agctggcgcg acgcctggat aaacgcgtcg
     2521 gcgatgcgcg gagccaccca ctccgcgtag ccctccgtgg cgggcgtgcc cagcgagctc
     2581 ttgatggacg gccccgtgtg cgtatgcgtg gcgcagatca tcacgcgctc cggcggcacc
     2641 ccggtctcgc tggcgatccg ctccttcgcg gcccggacaa catcgccggg cacggcgatc
     2701 aggtcgcaga ccacgatgcc gatcgtcatc tcgccattgc tgatggcgag cgccttggcg
     2761 tggagcgggt cgtgaatcgc cgtcgcgatg cggtcgttga agtagccgat cagatggcag
     2821 ccgagattgg gggtgatgtc gaccgatgct gaacctacct gaagcatgcg agagctcctt
     2881 tcgcccgtcg cctctcctgg gggcgggcaa ccgccctatt gtagccccgg cgcgaatggg
     2941 agtcaagcaa gatccaaggc ccggaggcgg cctgttatag cgcccgcggc aaccatgcgc
     3001 tcacgatgtg atgaggcctc gcgccggtcg atgaggcggc ttcctggcgg gtgcgtatta
     3061 gacgaggggt tgtgcccccc acgtgaggat tcttctcact cagatttaca aaatgctcgc
     3121 ttgaggtgtt gacagatctc tgatgatgcg ctataataag cgtgtcgttg ggcggggctg
     3181 tagcgcagtt gggagcgcgt ctcaatggca ttgagaaggt caggggttcg aatcccctca
     3241 gctccaccac aggcgagtcg gcaaaagcag gctcgccttt ttcattttct ggagagagat
     3301 ggagccgcac gccgatagca ccgaggagtt tgtcgttgtg atggagcccc gagactccgt
     3361 cgcggcgtca ctggccaagg ggatcctgga gaatgaggga atccccgtgc tggtcgagtc
     3421 gtaccagatc ccctggtacg acgggatcat gacgcccgcg accggcgcct ggggccgcat
     3481 cctggtgccc gcgcgcctcg catcgaacgc caaggcagtc ctctcggcct acttcgctga
     3541 gcaggaggat gaggccgcgc ctaccaccga tccagagtga gcgcctagcg ccaatccagc
     3601 gtgtcgaggc tcaagcccgc gagatggatc agggggcccg ccttgccaaa gggatcctgg
     3661 cgggccgcgg cacgcaggta agtcgtcgcc aggtagaacg gcaccgcggc acgatcgatg
     3721 ctgacgcgct cgcagtaatc ctccatcgcc atgcgcacgg cgcgtgagta ccagttctcc
     3781 tcacggtagg caaaacgcca ggcaacgccc gggtccatag ctccaccacc tgccagcagc
     3841 tccccgaggg catgggcaaa tcccagcaga tccatgagcg gcagcccctc cagagcgaga
     3901 tgctcccatc ccgtgacggc gatcccgtcc tcctggagga ggatgtggca cggccggagg
     3961 tgattgtgcg ccgcgcccag ccacaagccc gcttccgcga tgccgctcgc cactctcttc
     4021 acgaggggcc cccccgaagc cgcgccggcg gcgggaacca gcagtggccc gactttctcc
     4081 agcacggctt ctccctcgag ccacgcgctt gcgcgcgtct gctcgtggag ctctgccagc
     4141 caggtggcga ccgcctggag cgcgtgctcc gctacggccc gcgcctgcct cggactggcg
     4201 gccaccacct tctgatggag gggtaccccg gggatgtagg cgtgggccgc cgcgtggtgc
     4261 tctcgcacct cactggcccc gagtggctcg ggaagcgaat cgcgcaggcg ccccacaacg
     4321 cggctgcgca attctgccag tcccgcctgc ccgtcaccaa ccggtggcgc ctcgccacgc
     4381 caccggccgg cgcgcaggat gcaggccggg tggccccagc ccgccggcac caccagcagc
     4441 gcggcatgat cgccctcacc cgcatgcggg cgagaaagca cgaagttgag gtcgcgtgcc
     4501 ctcaaggcca cgcgcgcccg ctccgtcgcg ctcccccggc gccactcccc cgagcgcgga
     4561 cgtgcgacaa tgcgccggac catctcttcg gccatggtgg cacgcatgca tgccggcgcg
     4621 cgcgcgatta cccagaaccc aggacagatc caccgggccg cccgccattg cgagagcgca
     4681 gtcgtggcat agaggagcgt gctccgcgag gccgtgcctg gtcgaaggca ccgactgatc
     4741 aggcaccccg ccactcttcc ctgcccttcc ccacctacgg ggaaaaggat ctctggctga
     4801 tgagggtccg gcacggccgc caggatttgc ggattcggga aacctaccgc ctcaagcatc
     4861 cgatggagac gacgcgcgcc atattctccc caccacggcg accgcgcgcc acgcgctcgc
     4921 gtgcccggtt gcaacggcgt cacgccttca ggcggcttac ccgcagcaat cgccaggcag
     4981 ccctgcgggg caacgagctc cgccagccgc tcgaccgcct gcgccggcct caggccagcg
     5041 agcacacgcc gtgggtcacg caccaccacc agatcgaacc gccggcgggc gctgatgagc
     5101 gtatccaggt tcccgcggca cacgtggagg tgactcaacc ccgccgccga agcccgtcgc
     5161 cgggcaatct ggacgttcgc gggatccgga tctacagcca cgacctggtc gcagtaatgg
     5221 gccaactcaa gggagagcgc gccatcgccg cacccgagat ccaggccgac ggctcccgct
     5281 ccgagcggca gcaagaggag ccagtcgcca ctggccacgg ctggcgggtc tgtgggggca
     5341 tcccgcccgg cgagatcgac ctccgcgttc cagcagggca caggggagtg tttctcccgc
     5401 cggcgtgaat ccgacatgat ccgctccctc ccagaagccc ctgggctgca ggatgcatgc
     5461 caccctgcag cgataccacg gaaagggaac ggacatggat gcagagggca ctacacaggg
     5521 ggggcgccat catcccggat ttcgacaatg aggcttccgc gaagcgcggg cggaggaccg
     5581 cgaaggggcc gaggcacagc cacgcgcgag accatgaaga taggcgcttg gccgagcggg
     5641 aaagccggca tccaattccc ctttcgggag tgatgggacc ctttccacct caagcgcctc
     5701 acccacatag atgatagcac attgcgctcg gaagctcatg tcggaagagg aaaaggcccg
     5761 gatctggagt cgggagcctc taagcccagg agcggattcc ctacctcgga agcccggccg
     5821 attagctggg attccgacat aaggagaagg ccgcccgcac gcgtggagct cgaagctccg
     5881 cgctgcttct ttgcaaagcc ctttcagggc ttctcctccc agccaccgcc gttttgagac
     5941 atctgg
//
LOCUS       DAOSCV010000143         5931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8516_length_5931_cov_3.370490, whole genome shotgun sequence.
ACCESSION   DAOSCV010000143 DAOSCV010000000
VERSION     DAOSCV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8516_length_5931_cov_3.370490"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLY56_04935"
     CDS             <1..413
                     /locus_tag="PLY56_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20858.1"
                     /translation="PGMLTPYIKNDQVFECPSGGSIGAGYGGNQALFVTGTPVALAQI
                     AEPAETILIADASNRRGDIETGIGGDVFEGGYALKERTQANLDARINGTGCIGRGLFS
                     HRHLEMANIAFVDGHVKSMKYEATKTPKNLWDLQ"
     gene            complement(497..1198)
                     /locus_tag="PLY56_04940"
     CDS             complement(497..1198)
                     /locus_tag="PLY56_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20859.1"
                     /translation="MSGRRARGFTLIELLVVIAIIAILAAILFPVFARARENARKSNC
                     QSNLKQIGTALMMYVQDYDEYTPASWLGTNDQNGTIWPDLLVPYTKNMEMFNCPSSTI
                     KVGKDSVGKFLRTQIPYACNTAYWGGHAGSASGPVASHPMNKSMAELKVPAETVFVTD
                     FSGSWESGGQYDVNSAYAQPSTSTTFKAIYRHSDVCNVVFCDGHVKAMTQGALLQTHS
                     VSGVAVKYLFTIQED"
     gene            1785..4388
                     /gene="clpB"
                     /locus_tag="PLY56_04945"
     CDS             1785..4388
                     /gene="clpB"
                     /locus_tag="PLY56_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOJ20860.1"
                     /translation="MRLDKFTIKAQEAIQEAQRLAAQFGQQQIDVEHLLLALLQQEEG
                     VVPPILEKLGASRVPLIQAVQADLKSRPAVHGVAAGQGITPRLSRVLEQAWEEAERLT
                     DEYVSTEHLLLAIADSGDPADRLLKQAGVSKDGIYKAMEAVRGSHRVTDPNPEEKYQA
                     LERYGRDLTALARQGKLDPVIGRDDEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRVVAGDVPEGLKEKRVVALDLGALIAGTKYRGEFEDRLKAVLKEISESEGQIILF
                     IDEMHTIVGAGAAEGAVDAANMLKPMLARGELRAVGATTLDEYRKHIEKDAALERRFQ
                     PVLVGEPSVDDTIAILRGLKERYEVHHGVRISDPAVVAAATLSHRYITDRFLPDKAID
                     LIDEAASRLRMEIDSMPDEIDVLERRRIQLEIEREALRKESDPASLERLEKIERELSE
                     IAEESAGMKAQWQNEKAQILKIRELKEEIERAKIEEQNAERAGDLQRAAELRYGTRLN
                     LQKQLEQENARLAEIQSERKMLKEEVDAEDIAEVVSKWTGIPVSRMMEGEIQKLVHME
                     SRLRERVVGQDDAITAVSDAVRRARAGLGDPNRPIGSFIFLGPTGVGKTELARALAEF
                     LFDDERAMVRLDMSEYMEKHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYSVVLFDEI
                     EKAHPDVFNALLQLLDDGRLTDGQGRTVDFKNTVIIMTSNIGSAHIQELGGLNDQAMK
                     ERVLEAMRAHFRPEFLNRVDEIIVFRTLNAEQLKQIVEIQLERLKERLAERKINLEVT
                     DAAKDVIAAEGFDPVYGARPLKRVIQRRIENELAMRLLQGEFGEGATVRVDAASSGEL
                     LFTPVVEGTPA"
     gene            4818..5174
                     /locus_tag="PLY56_04950"
     CDS             4818..5174
                     /locus_tag="PLY56_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20861.1"
                     /translation="MRVIRPVSPGSITIVLCGLALVITLTTVYVVRHIHVSPPRSLNA
                     SFQSVEISVRDVNDWSLTQQATSHSQEKIAALVAVLRQARRTEDCKCGDIGTLLQQPG
                     AEPVRLGLVPGHERVL"
     gene            5158..5280
                     /locus_tag="PLY56_04955"
     CDS             5158..5280
                     /locus_tag="PLY56_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20862.1"
                     /translation="MSAYYEYRAYDGETFLGIYRVDRQALVSALKAFGVDLPPN"
     gene            5681..>5931
                     /locus_tag="PLY56_04960"
     CDS             5681..>5931
                     /locus_tag="PLY56_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20863.1"
                     /translation="MAGRTRSTVGPAAPSDSTAFIKARKALDQAARLERSGDLLGAIR
                     CCREGIASVADADDVEGLQLRIRLRMQCEAATRLHREAQR"
BASE COUNT         1142 a         1866 c         1978 g          945 t
ORIGIN      
        1 atccagggat gctcacaccc tatatcaaga acgaccaggt cttcgagtgt ccatcgggcg
       61 ggtccattgg cgcgggctac ggcgggaacc aggctctctt cgtcacggga acgccggtcg
      121 ccctggcgca gatcgccgag ccggccgaga cgatcctgat agcggatgcc agcaaccggc
      181 ggggcgatat tgagaccggg atcgggggcg acgttttcga aggcggctac gcgctaaagg
      241 agcggacgca ggccaatctg gacgcgcgga tcaatggaac ggggtgcatc gggcgcgggc
      301 tcttctcgca ccgccacctg gagatggcca acatcgcctt cgtagatggc cacgtcaagt
      361 cgatgaagta cgaggcgacg aagacaccga aaaacctctg ggacctccaa taggctagca
      421 aagccccgga gactggaaag gccggcgctc ctgcatgagc aggagcgccg gcccgatatc
      481 gcgccaagac ggccggctag tcttcctgga tcgtgaagag gtacttcacg gccacgccac
      541 tgaccgagtg cgtctggagc agggcgccct gggtcatcgc cttcacgtgc ccgtcacaga
      601 acaccacgtt gcagacgtcc gagtgccgat agatcgcctt gaaggtcgtg ctcgtggatg
      661 gctgcgcgta ggcagagttg acgtcgtact gcccgccgga ctcccagctg ccggagaagt
      721 cggtgacaaa aactgtctcc gcgggcactt tcagctccgc catcgacttg ttcatcgggt
      781 ggctggcaac aggcccgctc gcgctcccgg cgtgcccccc ccaataggcc gtgttgcacg
      841 catacgggat ctgagtgcgc aggaattttc ccaccgaatc cttgcccacc ttgatcgtgg
      901 acgacgggca gttgaacatc tccatgttct tcgtgtaggg cacgagcagg tccggccaga
      961 tcgtgccgtt ctggtcattg gtgcccagcc acgaggcagg ggtgtactcg tcgtagtcct
     1021 gaacatacat catcagcgcc gtgccgatct gcttcaggtt gctctggcaa ttggacttcc
     1081 gcgcgttctc tcgcgcccgg gcaaagaccg ggaagaggat ggctgcgagt atagctatga
     1141 tcgctattac caccaacaac tcgatgagcg taaacccgcg cgctctccta ccactcatgg
     1201 tgtctactcc ttactcctta actccttcgg gcagcctcca gagaggctcg tggatgcatg
     1261 gtagcattct cttagggagg ttgtcaagcg cgagcgccag cgggcgccgg ccccatccag
     1321 ccgcaccctg gtgtcgatac cacagatgaa gtcgggtgtg cttcacacgc gtgcggagct
     1381 cgaagctccg cgctactttc cggcaaagcc tcttcggggc tccccctccc agaccaccgc
     1441 caccgatccg cgggcggcgt gaaggggcga acgttcactg ttcgccatcc ttccggatgg
     1501 gttgggcagg cacccagggt gcggacaccc tgggctgcgt catgcgctcc ctccgggagc
     1561 ggagcagggc cagccgtgag gtcgcgaaag aagagacgca atagtttaca ataatgttat
     1621 caagtgatat aatagcaatg tgctaaatga cttgccatga ggcagcccgg gagcgatccg
     1681 agggtattga gtggaaaggg gtatcacgaa gaggcggggc aacgcgcgag aagcctcgcc
     1741 ggccagcgcg tctggggcgc ggcccaaacc ccgggaggcg agggatgaga ctcgataagt
     1801 tcacgatcaa ggcgcaggag gcgatccagg aggcgcagcg gctggcagca cagtttggac
     1861 agcagcagat cgatgtcgag cacctgctgc tggcgctcct gcagcaggag gagggcgtgg
     1921 tgccgccgat cctggagaag ctgggcgcca gtcgcgtgcc gctgatccag gcggttcagg
     1981 ccgacctgaa gagccgcccg gcggtgcatg gcgtagcggc tggccaggga attacccccc
     2041 gcctcagccg cgtcctggaa caggcctggg aagaagccga gcgcctcacg gacgaatatg
     2101 tctccacgga gcacttgctg ctcgccatcg ccgactctgg cgacccggcg gaccggttgc
     2161 tcaagcaggc cggggtgagc aaggacggta tctacaaggc gatggaagcg gtgcgcggct
     2221 cgcaccgggt caccgatccc aaccccgagg agaaatacca ggcgctcgag cgctacgggc
     2281 gagacctgac cgccctcgcc cgccagggta agctcgaccc ggtgatcggc cgcgatgacg
     2341 agatccgccg cgtgatccag gtcctctccc ggcgcaccaa gaacaacccg gtgctcatcg
     2401 gcgagccggg cgtgggcaag acggcgatcg tggagggcct cgcgcaacgc gtggtggctg
     2461 gcgatgtgcc cgagggcctg aaggagaagc gcgtggtggc gctcgacctc ggcgcgctca
     2521 tcgctggcac gaagtaccgc ggcgagttcg aggaccgatt gaaagccgtc ctcaaggaga
     2581 tctccgagtc ggaagggcag atcatcctct ttatcgacga gatgcacacc atcgtgggcg
     2641 cgggcgcggc ggagggcgcc gtggatgcgg ccaatatgct caagccgatg ctggcgcgcg
     2701 gcgagctgcg cgcggttggg gcgaccacgc tcgacgagta ccgcaagcat atcgagaagg
     2761 atgctgccct ggagcggcgc ttccagccgg tgctcgtcgg cgagccgagc gtcgacgaca
     2821 ccatcgccat cctccgcggc ctgaaggagc gctacgaggt tcaccacggc gtgcgaatca
     2881 gcgatcccgc ggtcgtcgcc gcggccacgc tcagccaccg ctacatcaca gaccgcttcc
     2941 tccccgacaa ggcgatcgac ctgatcgacg aggcagccag ccggctgcgc atggagatcg
     3001 actccatgcc ggacgagatt gacgtcctgg agcgccggcg gatccagctc gagatcgagc
     3061 gcgaggcgct gcgcaaggag agtgacccgg ccagcctgga gcgcctggag aagatcgagc
     3121 gcgagctctc ggagatcgcc gaggagagcg ccggaatgaa ggcgcaatgg cagaacgaga
     3181 aggcacagat cctaaagatc cgcgagctga aggaagagat cgagcgcgcg aagatcgagg
     3241 agcagaacgc cgagcgcgcc ggcgacctcc agcgcgccgc ggagctgcgc tacggcacgc
     3301 gcctcaacct gcaaaagcag ctcgagcagg agaacgcccg cctcgccgag atccagagcg
     3361 agcgaaagat gctcaaggag gaagtcgacg ccgaggatat cgccgaggtg gtgagcaagt
     3421 ggaccggcat ccccgtctct cggatgatgg agggcgagat ccagaagctg gtccatatgg
     3481 agagccgctt gcgcgagcgc gtggtgggcc aggatgacgc gatcaccgcg gtttcggacg
     3541 ctgtgcgccg ggcgcgcgcc ggcctgggcg atccaaaccg cccgatcggg agcttcatct
     3601 tcctcggccc aaccggcgtg ggcaagacgg agctggcgcg cgcgctggcc gagttcctct
     3661 tcgacgacga gcgcgccatg gtgcgcctgg atatgtcgga gtacatggag aagcacgccg
     3721 tcgcccggct gatcggtgcg cccccagggt atgtcggcta cgaggagggc gggcagctca
     3781 ccgaggcggt gcgccgccgg ccctactccg tcgtgctgtt cgacgagatc gagaaggcac
     3841 acccggatgt cttcaacgcg ctcctgcagc tcctggacga cggccggctg accgatggcc
     3901 aggggcgcac ggtggacttc aagaacaccg tgatcatcat gacgagcaac atcggcagcg
     3961 cccacatcca ggagctgggc ggcctgaacg accaggcgat gaaggagcgc gtgctcgagg
     4021 cgatgcgcgc ccacttccgg ccggagttcc tcaaccgggt ggatgagatc atcgtcttcc
     4081 gcacgctcaa cgcggagcag ctcaagcaga tcgtcgagat ccagctcgag cgcctgaagg
     4141 agcgcctggc agaacggaag atcaacctgg aagtgacgga tgccgccaag gacgtaatcg
     4201 ccgccgaggg gttcgacccg gtctatggcg cccggccgct caagcgtgtc atccagcggc
     4261 ggatcgagaa cgagctggcg atgcgcctgc tccagggtga gtttggcgag ggcgcaaccg
     4321 tgcgcgtcga tgccgcgtcc agcggcgagc tcctcttcac tccggttgtg gagggaacgc
     4381 cggcataacc cgaagtattc accagaatac ttcaagagaa ggctcttgtc tggtggcatg
     4441 ccgttgccgc tacaagcgcc cggaggcagg gtgttttcgg ggctgtgccc acgaacccgc
     4501 cccgcgcctg gcagagagac ttctttcatt attctgatga ataatgcggg ataaggcggc
     4561 agaaacgaaa cgcggggcca gggttggcgg cagccggccc tggccctacg cgttttgggc
     4621 accgtgcaac cgtgcttccg gcgcttgccc ccaggagttc gccgtgctat catacgtgcg
     4681 accgagcacc acacacccgg ttgtggggag ccgggcagtc gcttccggca gtcccgccgc
     4741 gcgggtgatt ccgcgccacg ccggaatgat ggctgctcgg atggtggcca ggtgcaatgg
     4801 cgaggggagg atgcctcatg agagtcataa ggcctgttag ccccgggagt atcacgattg
     4861 tgctgtgtgg cctcgcgctc gtgattacgc tcacgactgt ctacgtggtc cgccacatcc
     4921 acgtcagccc gcctcgaagc ctgaatgcct ccttccagtc ggttgagatc agcgtgcgtg
     4981 acgtaaatga ttggagtctt acccagcagg ccaccagtca cagccaggag aagatcgcgg
     5041 cgctagtagc ggtccttcgc caggcacggc gcaccgagga ttgcaagtgt ggcgacattg
     5101 gcactctcct ccagcaaccg ggcgccgagc cggtccgact cggcctcgtc cctggccatg
     5161 agcgcgtact atgagtatcg ggcatacgat ggggagacct tcctgggaat ctaccgagtg
     5221 gaccgccagg cgcttgtcag cgccctgaag gcctttggcg tcgatctccc gccgaactag
     5281 tggcgttccg ctttgccgtt gatcccaata cccatcgcgc tccctaaaga gcgcgaccgt
     5341 acgcgtcgat gccgcatcca gcggcgagct tctcttcacc ctggttgtgg acggagcgcc
     5401 agtgcccgca cgcggcacaa tcgcatcgga tccggcacac cgtgccaggc aatacggcgt
     5461 cagcgcgcgc ccattcgcca atcgcgcttc ataagaaggc gcgttcccgc ggcgcccgtc
     5521 tgtcccgaag ggagaagccg gtgagaggcg gcctgctcag cgagcaggaa cgaggcggcg
     5581 ggcgtcgaaa gtaagtatta gtacactccg cccgcgtggg aggtgcacgg cccgcgtcac
     5641 gcgtggtctc agcgcgagct gtaggaagaa gagcccgatc atggctggac gaacccgttc
     5701 cacagtcggc cccgcagcac cgagtgactc cacagccttc atcaaggcac ggaaagccct
     5761 tgaccaggcg gcccgcctgg agcgcagcgg agacctgctt ggcgccatcc gctgctgccg
     5821 cgagggcatc gcctctgtag cggatgccga cgatgttgag ggcctccaac tgcgcatccg
     5881 cctccgcatg cagtgtgagg cggccacccg ccttcaccga gaggcgcagc g
//
LOCUS       DAOSCV010000144         5928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8521_length_5928_cov_3.274476, whole genome shotgun sequence.
ACCESSION   DAOSCV010000144 DAOSCV010000000
VERSION     DAOSCV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8521_length_5928_cov_3.274476"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1913)
                     /locus_tag="PLY56_04965"
     CDS             complement(195..1913)
                     /locus_tag="PLY56_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20864.1"
                     /translation="MRGRRRLLAGALLTTLVISAGQLAGPKLKQIGIDDGIRAGDARL
                     LGWVVVVALGVHLATVVAGYFQASLLNRIGQDAIHDLRLRLFDHLQAQSLDYYEREQP
                     GQIISRILNDMDAINELLTSTVTALLADVVVLVGTSVILFRYHTGLALAIHFLIPLMF
                     LLGWAFRTRVHATFMRARQTIAAVTARLHESVAGMRVIQGFAREHRSCEEFGQLLQED
                     RDANMAGATVFATLSPGVELINALGICIVFWYGGLLILRGELAIGVAVAFILYLNHLF
                     DPVRRLSEMFATVQRAVVALERVFEVLDRKPTVTNAPGAVSIPPLRDGVEFRGVCFSY
                     DGAREILRDVSFRAGFGETVALVGPTGAGKSTILKLLARFYDPQAGEILLDGRDLRSV
                     TLESLRRQIGFVPQEPFLFAGSLRDNIRYGRPDAPDAEVERAARLAQAHDFIAALPEG
                     YDTDVHERGVRLSGGQRQLIAFARALLVNPRILILDEATSSVDPFTEKRIQDALRVLL
                     RDRIAFVIAHRLSTVVHADQILVVQDGGIAARGRHEELLYSCSLYRALYERRFQDTAE
                     EEAVVA"
     gene            2375..3274
                     /locus_tag="PLY56_04970"
     CDS             2375..3274
                     /locus_tag="PLY56_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20865.1"
                     /translation="MKRRIVITALLLGIGVATRGAVAGPLPAAVGNSVVARVDPALAK
                     EITAAQDGKGQPFTIALDGAQAEPLAISPVRLRDYQNVYYRVTGKPGAWQLEESGFSP
                     VGADEESRQIRRALGITDEKQVFFLPGREYLLLPAKSRAVIRVAGKSWSFRVSGSSAG
                     ERRPMKAILVRAGDPISLRLAEAVSSAATREGSSVRLTVAEPVMADGLIAVAQGTVLE
                     ARVIRVKRPSFPGNPGMVTLELGSVEAVDGTEIPLAVAGSPRVTFSARASFLLSRGQH
                     VTLAEGTPIAARAARAQAVLAPK"
     gene            complement(3286..4344)
                     /locus_tag="PLY56_04975"
     CDS             complement(3286..4344)
                     /locus_tag="PLY56_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ20866.1"
                     /translation="MWTLALLGLIQAPGDLIYRIQATAPVTLTLPACPEGEGHWIRAA
                     GKRWVRVKPTQAEGGLQFRLDPEEWEGGEALLVVGKTVGVDLADEQPPKVAALRVDGA
                     RVADAPVVDLDWRTAPPREIAWEVEDRSRLDTASLLVQVNGQAFAPGTPGIRVRPSSA
                     GRKLAIAVEPEKLPGIDARVETRVALSLADTSPTRNALERTLVYRRMAPPEGGKPVVA
                     HVDSCFEGYTPETLVDGKVMEPGTSTFGVTWASAETNTPHWAVLIFDKPRAVAGVELY
                     WAHFQGEYMVSTRYAVEAWDGARWRPLAEAKGQEPAKSTAHRFDPVTTTAIRVWQPAG
                     GGHPARPHLMWLTEIKPL"
     gene            4567..>5928
                     /locus_tag="PLY56_04980"
     CDS             4567..>5928
                     /locus_tag="PLY56_04980"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ20867.1"
                     /translation="MRIGLMATLILLLAIPAAVAFPLSDPTAPWVTAGAPGVAPTIDG
                     VVDAREWAGSVPLTGFTGVNTQELSSIQPTVRVARDAAHLYLAVHVPLPGGRSPVANA
                     RQHDGAVWEDDSVEVFLDPGHGHQEYFQFTGNAIGTRLDARGRDSSWNGDWRFQARAG
                     KGSWSAELAIPFATLQTRPPAENAVWGFNVCANIRGLGSSTWSPVLRSFHEPARFGHL
                     RFSTTLAAGVETLAGLIDGGGEIKGVARGTGIAMRWQLLRDGAQATEKRLSPAGEFTL
                     PVELPRDGRFPAAGRYSLRVVAERAGDKMPLMRLAADHTVSAPLELRARAFVNAGRLE
                     TEATVQAAGLDRASLACRFALAPEGGKPSVTREAALTDGRTVRATFQKAEVPEGKVSV
                     SVTLLEKGAPIRTISKVLDRPLSPPWLGDRTGITDEVPAPWTPLVVRGKQARAGARGS
                     AVSV"
BASE COUNT          998 a         2049 c         1950 g          931 t
ORIGIN      
        1 cccgtatagc ccagccaact aggcgctgcc cccgccggcg tgaccacggc caccgccagc
       61 accagagcga accacattct tctcaaggtc gcacctcctc gcatccacgg tgtcgccgcg
      121 cttgcagcgc actctcgcct gagtataccc gcggtatacg aggcccaaac caggcagccc
      181 ccgaggtgcg tcttctatgc tacaacagct tcttcttcag ccgtgtcctg aaaacggcgc
      241 tcgtaaagag cgcggtagag tgaacaggag tagagcaact cctcatggcg gccgcgcgcg
      301 gcaatcccgc cgtcttgcac gacgaggatc tgatccgcgt gcaccaccgt gctcagacga
      361 tgcgcaatca cgaaggcgat ccggtcgcgc agcagcaccc gcagcgcgtc ctggatccgc
      421 ttctccgtga aaggatcgac ggaggaggtc gcctcgtcca ggatcaggat gcgcgggttg
      481 accaggaggg cgcgcgcgaa agcgatcaac tggcgctggc ctccggagag tctcaccccg
      541 cgctcatgca catcggtatc atagccctcg gggagcgcgg cgatgaagtc atgcgcctgc
      601 gccaggcgag ccgcgcgctc cacctcggca tcaggcgcat ccggacgccc atagcgaatg
      661 ttatcacgaa ggctgcccgc gaagagaaag ggttcctggg gcacaaatcc gatctggcgc
      721 cgcagcgatt ccagcgtcac cgagcgcagg tcgcgcccat ccaacaggat ctcgccggct
      781 tgcgggtcgt agaaccgggc aagaagcttc aggatcgtgc tcttgcccgc acccgtgggg
      841 cccaccaggg ccaccgtctc tccgaagccc gcacgaaagc tcacatcgcg gaggatttcg
      901 cgcgccccgt cgtaggagaa gcagaccccg cggaactcca cgccgtcgcg caacggcggg
      961 atggaaaccg ccccaggggc gttggtcaca gtcggcttcc ggtccagcac ctcgaagacg
     1021 cgctccagcg cgacgaccgc gcgctgaacg gtggcaaaca tctcgctcag ccgccgaacc
     1081 ggatcgaaga ggtggttcag gtagaggata aacgccacgg ccacgccgat cgccagctcc
     1141 ccgcgcagga taagaagccc gccgtaccag aagacgatgc agatcccaag ggcattgatc
     1201 agctccacgc ccggggagag ggtggcgaag acggtggcgc cggccatgtt ggcatcgcgg
     1261 tcttcctgaa gcagctggcc gaactcctca cacgaccggt gttcacgggc gaagccctgg
     1321 ataacgcgca tcccggccac gctctcatgc agccgggcgg tgacggcggc gatcgtctgc
     1381 cgcgcgcgca tgaaggtggc atgcacccgc gttcgaaacg cccatcccag gaggaacatc
     1441 agggggatga ggaagtggat tgccagcgcg agcccggtgt ggtagcgaaa caagatcacg
     1501 ctggtgccca ccagcaccac cacatccgcc aggagcgcag tgacggtgga tgtcagcaac
     1561 tcgttgatcg catccatgtc gttcaggatg cgcgagatga tctgccccgg ctgctcgcgc
     1621 tcatagtaat cgagcgattg ggcctgcaag tggtcgaaga ggcgcagccg caggtcgtgg
     1681 atcgcatcct gcccgatgcg gttcagcaga ctggcctgaa agtagccggc cacgaccgtg
     1741 gccaggtgaa cccccagcgc cactacaacc acccacccga gcaagcgcgc gtcgccagcg
     1801 cggatcccgt catcaatccc gatctgcttc agcttcggac cggcaagctg cccggccgag
     1861 atcaccagcg tggtgagcag cgcgcctgcc agcaaccgcc tacgcccccg caagagcgaa
     1921 agtcctcgcc atagaacgtg cgggggcgtg cgggtatcag aatctggaga gttcgccatc
     1981 gtgttcgcgt gtgcgctcct attcttcctc atatacagta tgatacgcat ccgccaagaa
     2041 ccggccggga tcgagtatat catgattgcc gcggcggtgc aaagagaccg cgcactcctg
     2101 tcggcagcct aacccgcggc cccttcccca ccctccggcc cggcgccaac cccagggaag
     2161 aggagctacc gcgcggctct cgcggtggat cccgaaggga tccgacattc cagcctaggg
     2221 tctcctgacc ctgggtctcc ggaccgcccc ctctccatct gtccgcccgc cggtggaagc
     2281 gagcgaaaga ccctgttggg attgccatct ccgccgataa tcgatagtag caggcccccg
     2341 cgcgcggggg agccgcgaga ttggaggaac ggctttgaag aggcgaattg tcatcacggc
     2401 gctgttgttg gggataggtg tcgccacgcg cggcgcggta gcggggccac tgccggccgc
     2461 ggtaggcaac tcggttgtgg cgcgcgttga tccggccctc gccaaggaga tcacggcggc
     2521 gcaggatggg aagggtcaac ccttcaccat cgccctcgat ggcgcgcagg cggagcccct
     2581 cgcgatctcg ccggtgcgcc tgcgcgatta ccagaacgtc tattatcgcg tcacgggcaa
     2641 gccgggggca tggcagctgg aggaaagcgg cttctccccg gtgggcgccg atgaggagag
     2701 ccgccagatc cgccgtgcct tgggaatcac ggatgagaag caggtcttct tccttcccgg
     2761 gcgcgagtac ctccttcttc cggccaagtc gcgcgccgtc atccgggtgg caggcaagag
     2821 ctggagtttc cgggtctccg gctcctccgc gggcgagcgc cggccgatga aagcgattct
     2881 ggtgcgcgcc ggcgatccga tctcactccg tctcgcggaa gcggtgagca gcgcggcgac
     2941 ccgcgaaggg agcagcgtga ggctgacggt ggccgaaccg gtgatggccg atggcctgat
     3001 cgcggttgcc caaggcacgg tgctcgaggc gcgcgtgatc cgggtgaagc ggcccagctt
     3061 cccggggaac ccagggatgg taaccctgga gcttggcagc gtcgaggcgg tggatggcac
     3121 cgagatcccg ctcgcggtcg cgggatcgcc ccgggtcacc ttctctgccc gggcctcgtt
     3181 cctcctcagc cggggtcagc atgtgaccct cgcggaggga acacccatcg cggcccgtgc
     3241 cgcgcgggca caggccgtgc tggcgccaaa gtagagcagc cggcgctaaa gcggcttgat
     3301 ctccgtgagc cacatcaggt ggggccgcgc cgggtgcccg ccgcccgccg gctgccagac
     3361 ccggatcgcc gtggtggtca cgggatcaaa ccggtgcgcc gtgctcttgg ccggctcctg
     3421 cccctttgcc tcggcgagtg gacgccaccg cgcgccatcc caggcctcga cggcatagcg
     3481 ggtggaaacc atgtattcgc cctggaagtg ggcccaatag agctcgacac ccgccactgc
     3541 gcggggcttg tcgaagatga gcaccgccca atggggggtg ttcgtctcgg cagatgccca
     3601 ggtgacgcca aaggtcgacg tgcccggctc catcaccttg ccatccacca gcgtctccgg
     3661 cgtgtagccc tcaaaacagc tatccacgtg ggcgacgacc ggcttcccgc cttccggcgg
     3721 cgccattcgt cgatagacga gcgtgcgctc cagcgcgttg cgcgttggcg aggtatcagc
     3781 gagcgagagt gccacgcgcg tctccacccg cgcgtcgatg ccagggagct tctccggctc
     3841 cacggcaatc gccagcttgc ggcccgctga cgacggcctc acccggatgc ctggcgtgcc
     3901 cggcgcaaac gcctgcccat tcacctggac gaggagagag gcggtgtcga gtcggctcct
     3961 gtcttccacc tcccatgcga tctcgcgcgg tggcgcggtc cgccagtcga gatccaccac
     4021 tggggcatca gcgacacggg cgccatccac ccggagggcc gccacctttg gcggctgctc
     4081 gtccgcgaga tcgacgccca ccgtctttcc aacgaccaac agcgcctcgc cgccctccca
     4141 ttcctccgga tccaggcgga actggagccc gccctctgcc tgcgtcggct tcaccctcac
     4201 ccaccgcttg ccggcagcac ggatccagtg gccttccccc tcggggcagg ccggcagcgt
     4261 gagcgtgacc ggagcggtcg cctggattcg atagatcaga tcaccgggcg cctggatgag
     4321 gcccagcaac gcgagagtcc acatcgcttc tcctcctatt gagcaggatc ttcgccgcca
     4381 aggggcagca tcctttgtat ggccggcgtc aatccgacac tcgcgactgg aatgcccgct
     4441 caccagggcc tgccgggccg tggtggcgct cgcagccata cgccttcggg caaggaccgg
     4501 cgcgcctcgg gggcgaagga tgtagcatgc cggcggttgc atcctggccg gcggaggagg
     4561 caccctatgc gtattggtct aatggccaca ctgatacttc tgctcgccat tccggcggcg
     4621 gtcgcgttcc ccctgagcga cccgacggcg ccatgggtca ccgctggcgc ccccggagtg
     4681 gcgcctacca tcgatggagt tgttgatgcc cgggagtggg ctggctccgt gccgctcacc
     4741 gggtttacgg gcgtgaacac gcaggaactc tcttcgattc agcccacggt acgcgtggca
     4801 cgcgatgcgg cacatctgta cctggcggtt cacgtgccgc tcccaggggg ccggtcgccc
     4861 gtcgccaacg cccggcagca cgatggcgcc gtctgggaag acgactcggt ggaggtcttt
     4921 ctcgatccgg gccacggcca ccaggagtac ttccagttca ccggcaacgc cattggcacg
     4981 cgcctggacg cgcgcggccg ggatagctcc tggaatgggg actggcgctt ccaggcacgc
     5041 gcgggcaagg gcagctggag cgccgagctc gcaatcccgt ttgccacgct ccagacgcgc
     5101 ccgcccgccg agaacgcagt ctggggcttc aatgtgtgcg cgaacatccg cggcctcggg
     5161 agtagtacct ggtcgccggt gctccgctct ttccacgagc cggcgcgttt cgggcacctg
     5221 cgcttctcca ccacgctcgc ggcaggcgtc gaaacgcttg ccggcctgat tgacggcggc
     5281 ggtgagatca agggcgtggc ccgcggcacg ggcattgcca tgcgctggca gctcttgcgc
     5341 gatggcgccc aagcgaccga gaagcggctg tcgcccgcgg gcgagtttac cctcccggtg
     5401 gagctgccgc gcgacggccg attcccggct gcagggcgct actcacttcg ggttgtcgcc
     5461 gagcgcgctg gcgataagat gcccctgatg cgcctggcag ccgaccacac tgtctccgca
     5521 cccctggagc tgcgggcacg cgccttcgtc aatgcaggcc gtctcgagac ggaggcaacg
     5581 gtccaggctg ccgggctgga tcgcgcgagt ctcgcgtgtc ggtttgcgct cgcgcccgag
     5641 ggagggaagc cctcggtgac gcgcgaagcc gccctgaccg atgggcgcac cgtgcgtgcg
     5701 actttccaga aggcggaggt tccggagggg aaggtctccg tctccgtcac gctcctggag
     5761 aagggcgctc ccatccgcac catcagcaag gtgctcgacc ggccgctctc gccgccctgg
     5821 cttggcgacc gcaccggaat caccgatgag gttccggcgc cgtggacgcc gctcgtcgtg
     5881 cgcggcaagc aggcacgcgc tggcgcgcgc ggttccgccg tctcggtg
//
LOCUS       DAOSCV010000145         5923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8532_length_5923_cov_3.561691, whole genome shotgun sequence.
ACCESSION   DAOSCV010000145 DAOSCV010000000
VERSION     DAOSCV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8532_length_5923_cov_3.561691"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="PLY56_04985"
     CDS             <1..1315
                     /locus_tag="PLY56_04985"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ20868.1"
                     /translation="VRTSPLLALKGPQGTDLMQEYGRRWYELLERKPTRAPLVGWNSW
                     DFYSGAVTRQAMDETLDAARERFGDTLKAFVIDEGWERQWGSWEANGKFPEGLADFCR
                     HVKSRGGIPGIWTAPLLVNIYNPLYLEKPEWFAERADGQVQTDSYSYGPMAYLDPTVP
                     EVIEHLKGIFRRLRADGFEVFKVDFSHCILNAVRFRDRRVGRADLIRRAFTAIREAIG
                     PEAYLLSCGSPYESVVGLADAVRTTGDIHIYWGHILRNAHGISGRWWMQGNLWNGDPD
                     FLVVRGPDTAAPPYGRRRVVVPTGPDGGWMSGAEFNEMEARTYALLVHLSGGDVILGD
                     ALHKLNDTGLSIVKRVLEPRRPAAVPVDLFESEQEIPRIWISRGEKDTLVGLFNWSDK
                     PALLSFDPAAYGLTGTPRDFWTDAPVTTLPARMPRRSSLGLRFPA"
     gene            1718..2194
                     /locus_tag="PLY56_04990"
     CDS             1718..2194
                     /locus_tag="PLY56_04990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ20869.1"
                     /translation="MMRCDAQYYWCRLDISSRPENAAVVRRAVERLAIEAGLTPAETD
                     DLDLAVTEACANAIRHGSPEKEQSTVRVTFFLAPECVLVEVHDQGPGFTEGSRRAPAA
                     GELREGGYGLHIMRQLMDDVEIIWHRGTTVRLVKQRVPESAPRVSGRAARPIPAFV"
     gene            2285..3208
                     /locus_tag="PLY56_04995"
     CDS             2285..3208
                     /locus_tag="PLY56_04995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ20870.1"
                     /translation="MVREHPGRCRCIIIGGGPAGLAAALYFARFNCEALVISLEDQRA
                     RWIERTWNVPGYSEGIRGDELIARCREHALKYGATLIHGEVDEVTGRNNDFRVHLVDG
                     RVFYGRHIVFATGVRDTPPNFPNAYKYLGRGLRHCPVCDGYEANGKRLAVFGTGDRVA
                     RRALFLSTFTDHVTILLNGEGSFHEIDPSLRESLENRGIPCLEPPVVSVMDEGTEIRG
                     FLLQDGTRIEVDRAYSAQEIHPRSEVAAKMGVALDERGFIQVDAHCATNIEGVYAVGD
                     VAGTGYAQIIIAMGQAAIAAIHIHGRFDAPG"
     gene            complement(3253..3906)
                     /locus_tag="PLY56_05000"
     CDS             complement(3253..3906)
                     /locus_tag="PLY56_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ20871.1"
                     /translation="MLNISTEMLQRHGRLFEAGQAIFAEGERGDEMYLIHEGKVRIHR
                     RMGGRETTLAVLPPGECFGEMALIDDGPRSASATALTRTQLVVIARSTFETLVSTNSS
                     IFLGLLRKLSERLRRANRQIQVLLMRNDTARVAATLLLLTGEGNPGHEGIVLDRKATM
                     ETLEFMTGVSEKEIERVFDTLQNAAIARMAKREIVVLSSQGLEHFVDYLERRDRLGA"
     gene            4265..4411
                     /locus_tag="PLY56_05005"
     CDS             4265..4411
                     /locus_tag="PLY56_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20872.1"
                     /translation="MPKNESDRERSRRRKRKEERAREAKKAAIAEAATKPKKRKPVAA
                     AATV"
     gene            complement(4622..5464)
                     /gene="nadC"
                     /locus_tag="PLY56_05010"
     CDS             complement(4622..5464)
                     /gene="nadC"
                     /locus_tag="PLY56_05010"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOJ20873.1"
                     /translation="MLQRLQIEPIVRAALAEDIRSGDITTEATVPASLRARAEIVAKQ
                     EGVIAGLEVAALAFALLDPELRFDALAEDGDSVTPGQRVVSIEGRARAILMGERVALN
                     FLQRLSGIATLTRRFVDLVAGTGATIVDTRKTTPGLRLLEKYAVRAGGGQNHRYALDD
                     AILIKDNHIAAAGGITEAVRRARARGGHTVVIEVETQSREQVEEALAAGAGIIMFDNM
                     TPDQMRDCVALVGGRALTEASGGINEATARAVAETGVDLISVGALTHSAPALDLSLKI
                     CTAA"
BASE COUNT          964 a         1903 c         1988 g         1068 t
ORIGIN      
        1 cgttcgcacc tcgcccctcc tcgcgctgaa gggcccccag ggtacggatc tgatgcagga
       61 gtacggacgg cggtggtacg agctcctgga gcgcaagccg acccgcgccc ccctggtggg
      121 atggaatagc tgggacttct acagcggcgc cgttacccga caggcgatgg acgagaccct
      181 ggatgcggcg cgtgagcggt ttggcgacac actgaaggcg ttcgtcatcg atgagggatg
      241 ggagcgccag tggggaagct gggaggccaa tggcaagttc ccggagggcc tggcggactt
      301 ctgccggcac gtgaaatcgc gcgggggcat ccccgggatc tggacggccc ctcttctggt
      361 gaacatctac aacccgctct acctggaaaa gccggagtgg ttcgccgagc gcgcagatgg
      421 tcaggttcaa acggatagct actcctacgg cccaatggcc tacctcgacc cgaccgtgcc
      481 cgaggtgatc gagcacttga aggggatctt ccgccgcctg cgtgccgacg gtttcgaggt
      541 cttcaaggtc gatttctcgc actgcatcct caacgccgtg cgcttccgcg atcggcgcgt
      601 gggccgcgcc gacctcatcc gccgcgcctt caccgccatt cgggaagcca tcggccccga
      661 ggcctacctc ctctcctgcg gctcgcccta cgaatccgtt gttggtctgg cggatgccgt
      721 gcgtacgacg ggcgacatcc atatctactg ggggcatatc ctgcgcaacg cccatggcat
      781 ctcggggcgg tggtggatgc agggcaacct gtggaacggc gaccctgatt tcctcgtggt
      841 acgcggcccg gacaccgccg cgccccctta cggccggcgg cgtgtggtgg taccgaccgg
      901 gccggatggc ggctggatgt ccggcgcgga gtttaacgag atggaggcgc gcacctatgc
      961 cctcctggtg cacctcagcg gcggcgatgt gatcctggga gacgcgctgc acaagctgaa
     1021 cgacaccggt ctttccatcg tgaagcgcgt gctggagccc aggcgcccgg cggccgtgcc
     1081 ggtcgatctc ttcgagagcg agcaggagat cccgcgcatc tggatcagcc gcggcgagaa
     1141 ggacaccctc gtcggcctct tcaactggag cgacaagccg gctctcctga gcttcgaccc
     1201 ggcggcctac ggtctcaccg gcacgcctcg cgacttctgg acggacgcgc cggtcacgac
     1261 gctgccggcg cgcatgcccc ggcgcagctc ccttggcctg cgtttccccg cctaatcccc
     1321 cgatagggag cggagtgccg ccttcagctg gttgagccac actggcggaa ggccggcact
     1381 cccaggcagt ttgccgggag aaaccgcggg ctccatcgta ggccaggact acccccggcc
     1441 ggaatcccca acctatggta cggcgcttcg ccccatccat ccgcgatggg tggatgtccg
     1501 aagcgccgta tctcgtgtgg cacaactcct gcatccaatg ggcgcgtcga ctcggggcgc
     1561 tgcttgtcgg ctggtgggcc ttcccggcgg tgggcggcaa cggggcctac cggggagatg
     1621 cacttcgttg ctgcaaaccg cccgtcttgc cgccccaccc acttgacagg ttagcgcctg
     1681 gccttgttgg gtatgagaga acgagggtat tccggctatg atgcgttgcg atgcgcagta
     1741 ttactggtgc cgtctcgaca tctcgagccg gccggagaac gcggccgttg ttcgccgcgc
     1801 cgtggagcgt ctcgccatcg aggcgggatt gactcccgcg gagacggacg accttgatct
     1861 ggcggtgacg gaagcatgcg ccaacgccat ccgccacggc tcgcccgaga aggagcagag
     1921 cacggtccgc gtcaccttct tcctggcgcc cgagtgcgtc ctggtcgagg tgcatgacca
     1981 gggccccggg tttaccgagg gatcgcggcg cgcgccggcc gctggtgagt tgcgggaagg
     2041 ggggtatggc ctccacatca tgcggcagct catggatgat gtggagatta tctggcatcg
     2101 aggcacgacg gtccgcctcg tgaagcagcg cgtgcctgag agcgccccga gggtgtcggg
     2161 gcgtgcggcc cggccgattc ctgccttcgt ttgagcgccc gggcggtcga acgtgacggt
     2221 gccgccataa gcgcacagat caaaagcctg ccagagcagg gagtgaggta tgggggagag
     2281 tggaatggtc agggagcatc caggacgctg ccgctgcatc atcatcgggg gtgggccggc
     2341 aggcctcgcc gccgctctct actttgcccg cttcaactgt gaggcgctgg tgatctcgct
     2401 ggaggaccag cgcgcccgct ggatcgagcg cacctggaac gtcccggggt attccgaggg
     2461 aatccgcggt gacgagctga ttgcgcggtg ccgcgagcac gcgctgaaat atggggccac
     2521 cctgatccat ggcgaagtgg atgaggtgac cggccgaaac aacgatttcc gggttcatct
     2581 ggtcgatggc cgggtgtttt acgggcgcca tatcgtcttt gccacgggcg ttcgcgatac
     2641 gccccccaac ttccccaacg cctacaaata cctcgggcgg ggcctgcgcc actgcccggt
     2701 gtgcgatggg tatgaggcga acgggaagcg cctggcggtt ttcggcaccg gcgaccgggt
     2761 agctcggcgc gcgctctttc tctccacctt caccgatcac gtgacgattc tcctcaatgg
     2821 cgagggctct ttccacgaga tcgatccctc gctaagggaa tccctggaga accgtgggat
     2881 tccgtgcttg gagccgccgg tcgtgagtgt catggacgaa ggtaccgaga tccgcgggtt
     2941 tctcctgcag gatggcacgc ggattgaggt ggaccgcgcc tacagtgccc aagagatcca
     3001 cccgcgatcc gaagtggcag cgaagatggg cgtggcgttg gacgagcggg gctttatcca
     3061 ggtggatgcc cattgcgcca ccaacatcga gggcgtctac gccgtgggcg acgtggccgg
     3121 aaccgggtac gcgcagatta tcatcgctat ggggcaagcg gcgattgccg ccatccatat
     3181 ccacgggcgc ttcgatgccc ccggctaggc ctggggcagg ggagagtgcc catcttgcgg
     3241 tttgatggtg cgtcatgccc ccagccggtc gcgccgttcc aggtagtcta caaagtgctc
     3301 caggccctgg gatgagagga ccacgatctc ccgctttgcc atgcgcgcga tcgccgcgtt
     3361 ttggagggta tcgaagacgc gctcgatctc cttttcggag acgccggtca tgaactccag
     3421 cgtctccata gtcgctttcc tgtccaggac gatcccttca tggccggggt tgccctcgcc
     3481 tgtgagaaga aggagggtgg cggcaacgcg cgccgtgtcg ttgcgcatca gcagcacctg
     3541 gatctggcgg ttggcgcgac ggagccgctc gctgagcttg cgcaggagcc caaggaagat
     3601 cgaactgttg gtagagacga gcgtctcgaa ggtgctccgc gcgatcacga cgagctgagt
     3661 gcgtgtgagc gccgtggcgc tggcagaacg cgggccatcg tcgatcaacg ccatctcgcc
     3721 gaagcactca ccggggggta gcaccgccag cgttgtctca cgccctccca tccgtctgtg
     3781 gatgcgcacc tttccctcgt ggatgaggta catctcatcc ccgcgctcgc cctcggcgaa
     3841 gatcgcctga cccgcctcga acaggcgacc atgtcgttgg agcatctctg tgctgatgtt
     3901 gagcatcttc tgggatcccc tatagtatct ttttcggcgc atgcgggcgg aaactcaagc
     3961 gcccttccct tgtgcttctg ggattccgag agaggaaatg cgccgcgggg cgcccggttc
     4021 cggcgtggtg tcgcagtcgg cccggggttg gaaagccgtg agacccgcac acttccatcc
     4081 gcatccgtcc gcttcatccg attccgggtg cgagattccc caggagggcg agggcaactg
     4141 accggagcgg cctcatctcg gaatcccacg cggaaggcca ctccgcgttg acaggattgg
     4201 cggcgtggct tataataggc gcgttggagc ccaattgggg caggagggga ggggccaggg
     4261 aagcatgccg aagaatgagt cggatcgcga gcgctcacgg cgccgcaagc gcaaggaaga
     4321 gcgtgcgcgc gaggccaaga aggccgccat cgccgaggcg gcaaccaagc ctaagaagcg
     4381 gaagccggtc gctgccgccg ccaccgtgta aactaagtat ctattcgaat cctatggaag
     4441 cgcctcccgg cagctctgcc ggtggggccg cgccatctct ggcgctttcc ccatggccca
     4501 ggggcgatgg ggttatgtgc gttttcgcga gtgcgtgacg ggtgcctgcg tgggtgcggc
     4561 gcgcacaggt cgcccgggcc cgtcacgtat ccgcgaaacc ccgcgcgagc cactgccgga
     4621 cttatgccgc ggtgcagatc ttcaggctga ggtcgagagc gggcgccgag tgagtgagag
     4681 ccccgacgga gatcagatcg actccggtct ctgccacagc acgcgcggtt gcctcgttga
     4741 tgccgcccga agcttcggtg agcgcccggc cgcccaccag ggcgacgcag tcgcgcatct
     4801 ggtcgggggt catgttatcg aacatgatga taccggcgcc tgcggcaagc gcctcctcta
     4861 cttgctctcg gctttgcgtc tccacctcaa tcaccaccgt atgccccccg cgggcgcgtg
     4921 cgcggcgcac tgcctcggtg atgccgccgg cggctgcaat gtggttgtcc ttgatcagaa
     4981 tcgcgtcatc tagcgcgtag cggtggttct gcccgccgcc ggcgcgcacg gcgtacttct
     5041 ccagcaggcg caggcccggc gtcgttttcc gggtatccac gatggtggcg cccgtgcccg
     5101 cgaccaggtc cacaaaccgc cgcgtgaggg tagcgatccc ggagaggcgc tggaggaagt
     5161 tgagcgccac gcgctcgccc atcaagatgg cgcgtgcccg accctcgata gagacgacgc
     5221 gctgcccggg agtgacggag tcgccatcct ctgccagggc gtcgaagcgg agctccgggt
     5281 cgagcagggc gaaggccagg gcagccacct ccaggccggc gatgacgccc tcctgcttcg
     5341 ccacgatctc tgcgcgcgcc cgcaggctcg ccggcaccgt cgcctccgta gtgatatcgc
     5401 cagagcgaat atcctccgcc agggctgctc gaacgatcgg ctctatctgc aatcgctgca
     5461 acatctctct tccgctctcc tggaatgacc ccgctccgcc gcgctttcac cacctggccg
     5521 gcgggcgaac ctggaaatcg acctcctctg tggggtgata gccgtcgggt cactatccag
     5581 agattcgtga gagggtgcct cgctcctgcc gcgctgcatc cgcgtgcgtg tggagggcca
     5641 gggtgggggg acgacgaggg tgcctgctcc cttggtggaa catgcgcacc tctagtcaga
     5701 tgccccggag tgccggcatc agtgggtccg ggtcaaccgc ctgaagccag cactccgggt
     5761 gcacgcatgg ctaacgggtt gcggagggag tatcggatgg cctcacccgg cactccctgc
     5821 gttcactcac tgacctcgct ggctttggga tgccagcctg cgctcagttc agatcaaacc
     5881 acttgtctgt tgggttttgg ccgtagaaaa actgcgtggt ctt
//
LOCUS       DAOSCV010000146         5883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8596_length_5883_cov_3.470487, whole genome shotgun sequence.
ACCESSION   DAOSCV010000146 DAOSCV010000000
VERSION     DAOSCV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8596_length_5883_cov_3.470487"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLY56_05015"
     CDS             <1..437
                     /locus_tag="PLY56_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20874.1"
                     /translation="QHAEALKELLLAQRTPAGADDAARYRVIAHALDEEAQVLLAEAA
                     KTAEAARSGTLIREEAYTAVKTLVARADALAAEMSQLKAPGAVKDSHHRRTFAYSLLA
                     QCLFAYQGFYESGRATEGANAEALRAQAGRELERARETLGTP"
     gene            460..699
                     /locus_tag="PLY56_05020"
     CDS             460..699
                     /locus_tag="PLY56_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOJ20875.1"
                     /translation="MAGLGTIGKLLVGLGILLVGMGLLLMVMDRLTGGQGWRLPGDIL
                     IRRGNTTIYIPIATSILVSLLLTALLYLISWMSRR"
     gene            703..1716
                     /gene="queA"
                     /locus_tag="PLY56_05025"
     CDS             703..1716
                     /gene="queA"
                     /locus_tag="PLY56_05025"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ20876.1"
                     /translation="MMLSDFDYTLPEEAIAQEPVEPRDASRLLVMHRSTGTLEHRVFR
                     EIIDYLRPEDTLVVNDTRVIPARVHAVKETGGKVELLLLSRKGEGVWEALVKPGRRVP
                     PGTRLLAAGGAIQVDVLERTPSGGRLVRVSGAGDPERALLETGQVPLPPYIHRELADG
                     ERYQTVYAARSGSAAAPTAGLHFTKELLEQIRALGVSVVRVTLHVGLATFQPVRVEEI
                     TQHKMHVEQYEVSPEAAEQINATRGRVIAVGTTSTRVLESSADEAGRVRAGAGTTQLY
                     IYPGYRFRVVDAMVTNFHVPRSTLLIMVSAFAGREAVLHAYAEALQRGYRFFSFGDAM
                     LIL"
     gene            1926..3500
                     /locus_tag="PLY56_05030"
     CDS             1926..3500
                     /locus_tag="PLY56_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ20877.1"
                     /translation="MDAANAAQSRSIDRRTIEEIGIPSLVLMENAGLAVVAIARETLG
                     DVVGRTIVIFCGKGNNGGDGLVVARHLHRQGARVRVVLAAPREEFSGDAEVQARIATN
                     LGVPITQVRAADEVAELLAEADLAIDALLGTGTRGEVTGLVGDLIDLLNRSNVPAIAV
                     DIPSGLNAGTGVPCGRCVRAHATVTFGVMKRGLALYPGAALAGRVVLAPIGIPAEAIA
                     AERIGVSLVDAEAAQALLPRRDAWAHKGSAGSVLVVGGSAGMTGAATLVALSVLRAGG
                     GLVRLAVPNSLNDILETKATEAITLPVAETEARSFASEGLRQLVEVADASECVAVGPG
                     LGRHPETARAVLEWLPGVKAPLVLDADALNALADCPEVFGQLQAPAVITPHPGELSRL
                     LGQTVAEIQADRIAAAEEAARRFGVVTVLKGAGTVIADPKGPVFINSTGNAAMASAGM
                     GDVLTGAIAGLVAQGLPVTLAAVLGVYLHGLAGDIATEELGGPGILAGDVQDRLPAAA
                     ARLRAGRVPPAVSIRW"
     gene            3533..4915
                     /locus_tag="PLY56_05035"
     CDS             3533..4915
                     /locus_tag="PLY56_05035"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ20878.1"
                     /translation="MKILRTVRPAILFTMLLLLGVPARVAWAAGTLVVGMPSGEYTVW
                     LEYKDGKGAAQATPPVKVSGTKAAVDLAPLGSKFTDPVVVILDEKTGNVGTRAVEVKA
                     GETKEITLAESDITAVNTFRVCAVGEDGKPASGGAVTLTDSKGQGQTRPILPGRDGVV
                     EFERVPFGRTNVKVTFADGSTLAQDIEVKADHAPGAFSVELAKATPDGTPAASTEGES
                     GGEAAGAKEGEDSASEASSKQKEASPAKEGEAASDEEQQPASGAGGLQSLVALLILAG
                     IAYGGYRMLREKGVTAEQALGRLGVQIPPAEPAGDAAEKPAPPPVQVPEGACPYCGQQ
                     KDPATGACACSVTASPDAAGMAPPTAPIDSGAPRLVVVQGPALGGTVTLTDAPAVIGR
                     SAESDLPLVQDSTVSRRHASVIRQEGGYLIRDEGSSNGTFVNGQRVTERLLQLGDEIQ
                     VGSTRIRFEA"
     gene            4958..>5883
                     /locus_tag="PLY56_05040"
     CDS             4958..>5883
                     /locus_tag="PLY56_05040"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ20879.1"
                     /translation="MNSRLKILLEAMAGAAGGFVAWAIMEPIEFLTSDTQRLVADAAA
                     WWSLAIFGAIMGSAISAAIGAVEGLATGSRRQFWRSVVIGAVVGLGGGLIGLYFGQVV
                     YARLQPQPAGGALTVSLFGFLQGVVARAVGWALVGLAIGVAQGVPSWSPRKMKHGMIG
                     GGIGGFFGGMMFDFIGEIFQMGGELSRFVGMTITGAAIGFFLGLVQDLMKQAWVVVLR
                     GRNEGREYILDKAVSILGRDELAEVGVFGDPSVAPRHAAIKLDGGAYVIEDLGTSPGG
                     TLVNGQRVSKQGLREGDTIQLGATKILFHEKVG"
BASE COUNT          931 a         1749 c         2184 g         1019 t
ORIGIN      
        1 accaacatgc tgaggcgctc aaagagctgc ttctcgcaca gcggactccg gcgggcgccg
       61 atgatgctgc ccggtatcgg gtgatcgcgc acgccctcga tgaagaggcg caggtgctgc
      121 tcgctgaggc ggcaaagacg gcggaggcgg ccaggagcgg cacgctgatt cgcgaggaag
      181 cctacacagc ggtgaagacg ctggtcgcgc gcgcggatgc cctcgccgcc gagatgtctc
      241 aactgaaagc gcccggtgcc gtgaaggatt cgcatcaccg gcgtaccttt gcctacagct
      301 tgctggcgca gtgcctcttt gcctaccagg ggttctacga gagcggccgc gccaccgagg
      361 gggccaatgc cgaggcgctg cgcgctcagg caggccggga gctcgagcgg gcacgcgaga
      421 cgttgggaac gccctagcta tggaggagag cggtttggga tggcaggcct gggtacgata
      481 ggcaaactgc tggtagggct gggcatcctc ctggtcggga tggggctgct tctgatggtg
      541 atggaccgtc tcacgggagg gcaagggtgg cgcctccctg gagatatcct catccgtcgt
      601 ggaaacacga cgatctatat cccgattgcc acttcaatcc tggtgagcct gttgctgaca
      661 gctctcctct acctgatctc ttggatgagc cggcgctaaa tgatgatgct ctctgatttt
      721 gactatactc tgccggagga ggcgattgcc caggagccgg tagagccccg cgatgcctca
      781 cgactgctgg ttatgcaccg ctctacaggg accctcgaac accgggtgtt ccgcgagatc
      841 atcgactact tgcggcccga ggataccctc gtcgtcaacg atacccgagt cattccggct
      901 cgggtgcacg ccgtcaagga gacgggcggc aaggtggagc ttctcctcct atcgcgaaag
      961 ggcgagggcg tgtgggaggc gcttgtcaag ccagggagac gagtgccgcc cggaacgcgg
     1021 cttctggccg ccgggggagc tattcaggtg gacgtgctgg agcgcacccc atcgggaggg
     1081 cgactggttc gcgtttccgg ggcgggcgat ccggagcggg cgctgttgga gaccggccag
     1141 gtaccgctcc caccctacat ccaccgggag ctggccgatg gcgagcgtta ccagacggtg
     1201 tacgcagcgc gctcgggctc tgcggcggcg cccacggcgg ggctacactt caccaaggag
     1261 ctgctcgagc agatccgcgc gcttggggtt tccgtggtcc gggtcacgct tcatgttggc
     1321 ctggcgacgt tccagccggt gcgcgtggag gagatcaccc aacacaagat gcacgtcgag
     1381 cagtacgagg tctcgcccga ggccgcggag cagatcaacg cgacgcgcgg tcgggtgatt
     1441 gccgtgggca ccacctccac gcgcgtattg gagagcagcg ctgatgaggc tgggcgggtg
     1501 cgcgcgggcg cgggaacaac gcagctctat atctaccccg ggtaccgctt ccgggtggtc
     1561 gacgcgatgg tgacaaactt ccatgtgccg cgctcgacgc tgctgattat ggtcagcgcc
     1621 ttcgccggtc gcgaggccgt gctccatgcc tatgcggagg cgctgcagcg cgggtaccgt
     1681 ttcttcagct tcggtgacgc catgctgatt ctctaatgtg gggtggcgcg gccacggctc
     1741 tcctcggccc ttccggcagt gtggatgttc atccccgcgc ggtcggcacg ggtattgacg
     1801 cccgtgtgat agcggcggcg agcctgcgca ttccaatggg gtgttgttgc gggagacccc
     1861 tggatgtgct ataatggact tgcctgcccg ccctggtatc gtggtcctgc tgggagagag
     1921 aagccatgga tgcggccaac gccgcgcaaa gtcgcagcat cgaccggcgt accatcgagg
     1981 agatagggat cccctccctc gtgctgatgg agaacgccgg cctggcagtc gtcgccatcg
     2041 ctcgagaaac gcttggcgat gtggtagggc gcaccatcgt tatcttctgt gggaagggca
     2101 acaacggggg agatggcctg gtggtcgccc ggcacctgca ccgccagggt gcgcgcgtgc
     2161 gcgtcgtctt ggctgcgccg cgtgaggagt tttctggcga cgcggaggtc caggcgcgga
     2221 tcgcgacgaa tctgggcgtg ccgatcacgc aggtgcgcgc tgccgatgaa gtggccgagc
     2281 tcctcgcgga ggccgatctc gcaattgatg ccctcctggg cacgggtacg cgcggcgagg
     2341 tgacgggttt ggtgggcgac ctcatcgacc tgctcaatcg ctccaatgtg ccagccatcg
     2401 cggtagatat cccttccggt ctgaatgcgg gtacgggcgt gccgtgcggg cgttgcgtgc
     2461 gtgcccatgc aacggtcact ttcggtgtga tgaagcgagg gctcgcgctc tacccgggcg
     2521 cggcgctcgc gggcagagtg gtgctggcgc caatcgggat cccggcggaa gctatcgccg
     2581 cggagaggat tggcgtctcg ctggtagatg cggaggcggc ccaggcgttg ctcccaaggc
     2641 gggatgcctg ggcacacaag ggatcggccg gcagtgtgct cgtcgttggt ggctcggccg
     2701 gcatgacggg cgcagccaca ctggtggcac tttcggtgct gcgcgcgggc ggcgggctgg
     2761 tgcgtttggc cgttcccaat agcctgaacg atattttgga gaccaaagcg accgaggcga
     2821 tcacgctgcc ggtcgccgaa accgaggcgc gctcctttgc gtcggagggc ctgcgccagc
     2881 tggtggaggt ggcggacgcg tccgagtgtg tcgccgtcgg tccgggcctg ggccggcatc
     2941 cggagacggc acgggcggtg ttggagtggc tgcccggggt gaaagcgcct cttgtcctcg
     3001 atgccgatgc gctgaacgcc ctagccgatt gtcccgaggt gtttggccag ctgcaagcgc
     3061 cggcggtgat cacccctcac ccgggagagc tgagccggct gttgggccag acggttgcgg
     3121 agattcaggc cgaccgcatt gccgcagcgg aagaagccgc gcgccgcttc ggagtcgtca
     3181 ccgtgctcaa gggggccgga acggtgatcg ccgacccgaa gggccccgtc tttatcaatt
     3241 cgaccggcaa cgccgcgatg gcctcggcag gcatgggtga tgtgctcacc ggcgcgattg
     3301 ccgggctcgt ggcccagggg ctccctgtga ccctggcggc cgtgttgggg gtgtatctcc
     3361 acggtctggc gggcgatatc gccacggagg agctgggtgg ccccggtatc ctggcgggag
     3421 atgttcaaga tcgcctgcct gccgctgctg cgcgcctgag agcagggcgc gtaccaccgg
     3481 cggtatcgat tcggtggtga tcgcgccggc tttcacggag agggttccgc acgtgaaaat
     3541 actacgcact gttcgcccag ccatactctt cacgatgctc ctgctccttg gggttccagc
     3601 gcgtgtcgcc tgggccgccg gcactctggt ggtcgggatg ccctcaggcg agtatactgt
     3661 ttggctcgaa tacaaagatg gcaagggcgc cgcgcaggcg acgcctcccg tgaaggtctc
     3721 gggcacaaag gcagcggttg acctcgcccc gctggggagc aagttcaccg atcccgtggt
     3781 ggtgatcctc gatgagaaga cggggaacgt cggcacccga gcagtcgagg tgaaggcggg
     3841 cgagaccaag gagatcaccc tggccgagag cgacatcacg gcggtgaaca ccttccgggt
     3901 gtgcgccgtg ggcgaggatg ggaagcctgc cagcggcggc gccgtcaccc tgacggatag
     3961 caaggggcag gggcagaccc ggcccatcct gccggggcgc gacggcgtgg tggagttcga
     4021 gcgcgttcct ttcggtcgca ccaacgtcaa ggtcaccttc gcggatggtt cgacgctggc
     4081 gcaggatatt gaagtcaagg cggatcacgc gcccggcgcg ttctcggtgg aactcgcgaa
     4141 ggccaccccc gacggcacgc cggctgcttc gaccgagggt gaatctggcg gagaggccgc
     4201 aggcgcgaaa gagggcgagg actcagcatc agaggcttca tcaaagcaga aagaggcttc
     4261 gcccgcgaaa gagggcgagg ccgcgagtga cgaggagcag cagccagcat ccggcgcggg
     4321 gggcctccag agcctggtgg cgctgctcat cctcgcgggg atcgcctatg gcggctaccg
     4381 catgctccgt gagaagggcg tgacggcaga gcaggcgctg gggcgcctgg gtgtgcagat
     4441 cccgcctgcc gagccagccg gcgacgcggc ggagaagcca gcgccgccac ccgtccaggt
     4501 gccggaggga gcgtgccctt actgcggcca gcagaaagat ccggcgacgg gggcgtgcgc
     4561 gtgctccgtg acggcctcgc cggatgcggc cgggatggct cctcctacgg cccctatcga
     4621 tagcggggcc ccgcgattgg tggtggtgca gggcccggca ctgggaggta ccgtcacctt
     4681 gacggacgcg ccggccgtga ttgggcggtc cgcggagagc gacctgcctc tcgtgcagga
     4741 tagcaccgtg tcgcgccggc atgccagcgt gatccggcaa gagggtggct atctgatccg
     4801 cgatgagggg agctccaacg gcacattcgt gaatgggcag cgcgtgacgg agcggcttct
     4861 ccaactgggg gatgagatcc aggtcgggag cacgcggatc cgtttcgagg cgtagggatg
     4921 catgcggaat ggcgcccgcg ggtgccatgg gagggcaatg aactccaggc tgaagatact
     4981 gttggaggca atggccgggg cggccggcgg ctttgtcgcg tgggcgatca tggagccgat
     5041 cgagttcctg accagcgaca cgcagcgcct ggtggcggac gccgccgcct ggtggtcgct
     5101 cgcgatcttc ggcgcgatca tgggcagcgc gatcagcgcg gcgattggcg cggtggaagg
     5161 tctggcgacg ggatcgcgcc ggcagttctg gcggtcggtc gtgattggcg cggtcgtcgg
     5221 cctgggcggc gggctcatcg gcctctattt tggccaggta gtctatgcgc gcttgcagcc
     5281 ccagccggcg ggcggagcgc tgacggtcag cctctttggc ttcctgcagg gcgttgtggc
     5341 gcgggccgtg gggtgggcgc ttgtcggact ggcgatcggg gtcgcgcagg gggtgccctc
     5401 ctggtcgcct cgcaagatga agcacggcat gatcggcgga gggatcggcg gcttctttgg
     5461 cggcatgatg ttcgacttca tcggggagat cttccagatg gggggcgagt tgagccgctt
     5521 cgtgggcatg acgatcaccg gggcggccat cggcttcttc ctcggcctgg tgcaagacct
     5581 gatgaagcag gcctgggtcg tcgtcttgcg cgggcgcaac gaaggtcggg agtatatcct
     5641 cgataaggcc gtcagcatcc tcgggcgcga cgagctggcg gaagtgggcg tgttcgggga
     5701 tccctcggta gcgccccggc acgcggcgat caagctggat ggcggggcct acgtgatcga
     5761 ggatctgggg acatcgccgg gcggcaccct ggtgaatggg cagcgcgtgt ccaaacaagg
     5821 tctgcgcgag ggcgatacga tccagctcgg ggcgacgaag atcctcttcc atgaaaaggt
     5881 ggg
//
LOCUS       DAOSCV010000147         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8630_length_5866_cov_3.036999, whole genome shotgun sequence.
ACCESSION   DAOSCV010000147 DAOSCV010000000
VERSION     DAOSCV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8630_length_5866_cov_3.036999"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLY56_05045"
     CDS             complement(<1..790)
                     /locus_tag="PLY56_05045"
                     /inference="COORDINATES: protein motif:HMM:NF025615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HOJ20880.1"
                     /translation="MAKGARVLLPAEHGSWSLMLTPFLIGAGVAWVAGHETSGLALTL
                     ALVAVLALFLARQPLAAWLRARTRAPEAAAEAFRWGLAMLAVAALAGLGLLALGRGAV
                     LWLALPAAMLLAVTLAMSSWLGPRRLATELAGVAGLALAAPAAYVAGTGALDATAAGV
                     WAISALYSGLSVVYVRLRLHQRNERVSRGEGIWVVVAHLLGGAAVLAGIRAGWLPALL
                     ALPFGLMAARAAVAAWRNPPLESVRRFGFTEMALALCFAALAIFA"
     gene            967..1632
                     /locus_tag="PLY56_05050"
     CDS             967..1632
                     /locus_tag="PLY56_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ20881.1"
                     /translation="MGLDRKVTMAEKRKRLTAEERRERILDAALELFAEKGYAATTTR
                     ELARRAGITEPVIYLHFESKEAVLRGVFQRHSFLPYLREFAALEGQAPLRDQLLELGT
                     RWRRFIRERHHLVTFVIREIHRVPEMGRALRETLAAGLPLAAEAVRAHADLTGYRPER
                     LETAIRLFLSSLLQLFLLEGPAFFEDGSRIDALMEESVDLLVDGLRDPLGDAPAKATG
                     ARK"
     gene            1720..3330
                     /locus_tag="PLY56_05055"
     CDS             1720..3330
                     /locus_tag="PLY56_05055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ20882.1"
                     /translation="MSLTHSRVRVAVPVLAIAIVAGLLVYRAGMSRRAAETPEGVIIA
                     SGTVEMTQVAVSSKLSGRLERLLADEGDVVKCGQPLARLDARELEAKQAELEAAVAAA
                     EARLQELEKGARPEELARARAALARAEAAHSGALDAARTAREGHETSTELKRQVDTAE
                     ANLRAARAQEQQARARLSLLRAGPRPEELDQARAALASAKARLALARKGPREEEIQEA
                     EAGLAQAEAVLENAEATLKRTQKLFQEGAVARQQLDDALTARDRARAAVDAARARARA
                     LHAGTRPEEKEQAEAEVRRLEAQVALLEAGTREEEIAAAEAELRRAQANVEGCETALA
                     NARRLYRERLQARGQRETAEAQVRVTAHQVEEARAQLELLRAGATPEAVQAARAARDQ
                     AKAALDQLRARLDDARIMAPIDGVVAEQIAEEGEVISAGQSILTLVNPRDTWVKVYLP
                     VTLLGQIGVGTPARVRVDAFPGEEFPGRVVELSQIPEFTPKNVQTQEQRTQLVFWVKV
                     AVENSSGKLKPGLPADALFDGQGAGNGR"
     gene            3320..4255
                     /locus_tag="PLY56_05060"
     CDS             3320..4255
                     /locus_tag="PLY56_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20883.1"
                     /translation="MAAELAILAEGVRKRFGDVVAVDGVSLAIPRGEIFGLVGPDGAG
                     KTTMMRLLAALFPPDEGILRVAGHDIVRDPESVRPRLGYLSQRFSMYGELTVAENIAF
                     FAEMFGVPRAARREREAELLQMTRLAPFRDRRAQDLSGGMKQKLALICTLIHRPEVIL
                     LDEPTTGVDPVSRREFWKLLADLPSAGITLLVSTPYLDEAERCHRLGFMAQGRLLAVD
                     TPQALKQYAPAVIYRVRVEPLRVARDFLRGRPEVEGAELFGDRLHVSLREGDGKALAA
                     LLAGAGFTASPPERVAPSLEDAFVALTRAQEAAHA"
     gene            4248..5189
                     /locus_tag="PLY56_05065"
     CDS             4248..5189
                     /locus_tag="PLY56_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ20884.1"
                     /translation="MPEAIIEARDLVKRFGRFVAVDHLSLTVNRGEILGFLGPNGSGK
                     STTIRMLCGLLRPTSGSGSVMGYDITREAEQIKERIGYMSQRFSLYEDLTVAENVLFY
                     AGIYRVPRARRKERLEWVLEMAGLKEHRNRLTGTLSVGWKQRLALGCALAHEPEILFL
                     DEPTSGVDPVSRRGFWDLIAGLAEEGVTVMVTTHVMEEVEHCHRAVLIYQGRQIALGT
                     PAELKASLPGALYEVRVEPALAALKALERVEGVEDAALFGTALHVRTRTPIPEAMLEK
                     ALVDAGFPGARVTPAEVQLEDLFISLIAEADRSGKEA"
     gene            5192..>5866
                     /locus_tag="PLY56_05070"
     CDS             5192..>5866
                     /locus_tag="PLY56_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ20885.1"
                     /translation="MWRRLWAICRKEWIQIRRDPRTLAVIWLLPITQLLLFGYGMDFD
                     LKRVHVAVADYDRSQASRVLVAAFDQSEHFEVIPAAGRTAEVERMVVDARARLGVVIP
                     AGFERALRLGQPAPVQVLLDGADPTTGGTALGYTRGLIESFSREWLREHPIRAVAPAE
                     SGSDQAVFVSSRQPAALDVRARVLYNPELRSANFLVPGLMAVILTMIAALLTSNAVAR
                     EKERGTF"
BASE COUNT          952 a         2051 c         2022 g          841 t
ORIGIN      
        1 aggcgaagat cgccagcgcg gcaaagcaaa gcgccagtgc catctcggtg aacccgaacc
       61 ggcgcacgct ctccagcggc gggttgcgcc acgccgcgac cgccgcacgc gccgccatca
      121 gcccgaaggg aagcgccagg agagcgggaa gccaccccgc ccggatccct gccagaacgg
      181 ccgctccccc caggagatgc gccacgacca cccatatccc ctcgccacgc gatacccgtt
      241 cgttgcgctg gtggaggcgc aggcgcacgt agaccacgct aagcccgcta tacaacgcgc
      301 tgatcgccca gacacccgcc gcggtcgcgt cgagtgcccc cgttccggcc acgtaggccg
      361 ccggggcagc aagcgccagg ccggccacac ccgcaagctc cgtcgccagc cggcgtggcc
      421 ccagccacga gctcatcgcc agggtgaccg cgaggagcat tgccgcgggc agtgccagcc
      481 acagaaccgc gccccgcccc agggcaagca ggcccaggcc ggcgagcgcc gcaaccgcca
      541 gcatcgcgag accccaccgg aaggcctccg ccgcggcctc gggcgcgcgt gttcgagcgc
      601 gcagccacgc cgccagcggt tgccgcgcga gaaagagcgc cagcaccgcc acgagcgcga
      661 gcgtcagcgc caggccactc gtctcatgcc ctgccaccca ggccacgccc gcgccaatca
      721 ggaacggcgt gagcatcagc gaccacgacc catgctccgc tggcaggagc accctcgcgc
      781 ctttcgccat ccgatactct ccaagcctcc ccctcacggc ctctcaccac ggcttaccgg
      841 tggattcgcg gcgcaggccc ccatccctgc cgcaagaccg gcgcgccaat cgcaaacaat
      901 cgaaaacacc tattgacgcc gggagggcgt tggaatatac ttagctagtg attactaagt
      961 tcgctgatgg gcctggatag aaaggtgacg atggcggaga agcggaagcg attgacggct
     1021 gaggagcggc gcgagcggat cctggacgcg gcgctcgagc tgtttgccga gaaaggttac
     1081 gcggccacga ccacgcgcga gctggcccgg cgcgcgggga tcaccgagcc ggtcatctat
     1141 ctccacttcg agagcaagga agcggtgctg cgcggggtgt tccagcggca ctcgtttctc
     1201 ccctacttgc gcgagttcgc cgcgctcgaa ggtcaggcgc ctctgcgcga ccagcttctg
     1261 gagctgggca cccgctggcg gcgcttcatc cgcgagcgcc atcacctggt cacgttcgtc
     1321 atccgggaga tccatcgcgt gcccgaaatg ggccgcgccc tccgcgagac gctggccgcc
     1381 gggctgcccc tcgctgcgga agccgtgcgc gcgcacgcgg acctgactgg ctatcgcccg
     1441 gagaggctgg agacggcgat ccgcctcttc ctctcctcgc tactgcaact cttcctgttg
     1501 gaaggaccgg cgttctttga agacggctcg cgtatcgacg cgctcatgga ggagagcgtc
     1561 gaccttctgg tggatggctt gcgcgaccca ctgggcgacg cgcctgccaa ggcgacaggc
     1621 gcccggaaat aagcgaaggt cgatccattc gacgcgagcc ccctggcttg gatcaaggca
     1681 cgccgcgctc atcgcggcgg catgaggatc ggaaagaaga tgagtctcac tcactcccgc
     1741 gtgcgcgtgg ccgtgcccgt gctggcgatt gccatcgtcg cggggctgct ggtctatcgc
     1801 gcgggcatgt cgcgccgggc ggcagaaaca cccgaaggcg tgatcatcgc ttccggcacg
     1861 gtcgagatga cccaggtcgc ggtgagctcg aagctctccg ggcgcctgga acgcctcctc
     1921 gccgatgagg gcgatgtcgt gaagtgcggc cagccactcg cccggctcga cgcgcgcgag
     1981 ctggaggcga agcaggccga gctggaggcg gcggtcgccg ccgcggaggc gcggctgcaa
     2041 gagctggaga aaggcgcccg gcccgaggag ctggcgcgcg ctcgcgccgc gctcgcccgg
     2101 gccgaagccg cgcactccgg cgctctggac gcggcacgaa cagcccggga gggccatgag
     2161 accagcactg agctcaaacg ccaggttgat accgccgaag caaacctgcg cgccgcgcgt
     2221 gcccaggagc agcaggcgcg ggcgcggctc tccctcctgc gcgccggccc ccggcccgag
     2281 gagctggacc aggcccgcgc cgccctggcc tccgcgaagg cacgcctggc gctcgcgcgg
     2341 aaaggccccc gggaggagga gatccaggag gcggaggccg gcctggccca ggccgaggcg
     2401 gtgttggaga acgctgaagc cacgctgaag cgcacccaga agctgttcca ggagggcgcc
     2461 gtggcccgcc agcaactgga tgacgccctc accgcccgag accgcgcccg tgccgccgtg
     2521 gatgccgctc gggcccgggc gcgcgccctc cacgctggaa cccggcccga ggagaaggag
     2581 caggccgagg ccgaagtccg ccggctcgaa gcgcaggtcg ccctcctgga agcgggaacg
     2641 cgcgaagagg agatcgccgc cgcggaagcc gaactgcgcc gggcgcaggc aaacgttgaa
     2701 gggtgcgaga cggcgctcgc caacgcgcgt cggctctatc gcgagcggct tcaggcgcgc
     2761 gggcaacgcg agaccgcgga ggcacaggtc cgcgtcactg cgcaccaggt ggaggaagcg
     2821 cgcgcccagc tggagctgct tcgggccggt gcaactcctg aggcggttca agccgcgcgc
     2881 gccgcccgcg accaggcgaa ggcagccctc gatcagcttc gcgcccgcct ggatgatgcc
     2941 cggatcatgg cgccgatcga tggcgtggtc gccgagcaga tcgccgagga gggcgaagtc
     3001 atctccgcag ggcagagcat cctcacgctg gtcaacccgc gcgacacctg ggtgaaggtc
     3061 tatctcccgg tcacgctcct cgggcagatc ggcgtgggga ctccggcacg agtgcgcgtc
     3121 gatgcctttc cgggagagga gtttccgggg cgcgtggtcg agctgagcca gatccccgag
     3181 ttcaccccga agaacgtgca gacgcaggag cagcgcaccc agctcgtctt ctgggtgaag
     3241 gtggcggtgg agaactcgag cgggaagctg aagccgggcc tacccgccga cgcgctcttc
     3301 gatggccagg gagcgggcaa tggccgctga gctggcaatc ctggccgagg gcgtgcgcaa
     3361 gcgcttcggc gacgtggtcg ccgtggacgg ggtgtcgctc gccatccccc ggggagagat
     3421 cttcggcctg gtcggcccgg acggcgccgg caagaccacg atgatgcgcc tcctggcggc
     3481 gctcttcccg cccgatgagg gcatcctccg cgtcgccggg catgatatcg tgcgcgaccc
     3541 ggagagcgtg cgcccgcgcc tcggttacct ctcgcagcgc ttcagcatgt atggcgaatt
     3601 gaccgtcgcc gaaaacattg ccttcttcgc cgagatgttc ggcgttcccc gggccgcgcg
     3661 gcgcgagcga gaggccgagc tccttcagat gacgcgcctg gcgcccttcc gtgaccggcg
     3721 tgcacaagac ctttctggtg gcatgaagca aaagctcgcg ctgatctgca ccctcatcca
     3781 ccgtccggag gtgatcctgc tcgacgagcc gacgaccggc gtcgatcccg tctcacgccg
     3841 ggagttctgg aagctcctcg ccgacctgcc atcagcggga atcaccctcc ttgtctccac
     3901 gccttacctg gatgaagcag agcggtgcca ccgccttggc ttcatggctc agggccggct
     3961 gctcgcagtc gatacccctc aggcgctgaa gcaatatgcc cccgcggtga tctaccgggt
     4021 tcgtgtagag cccctgcgcg tggcgcgcga cttcctccgg ggccgaccgg aggtagaggg
     4081 cgccgagctg tttggcgacc ggcttcacgt ctcgctccgc gaaggagacg gcaaggcgct
     4141 cgcggcgctt cttgccggtg ccggcttcac ggcttcgcct ccggagcgcg tggcgccgtc
     4201 actggaagac gcgttcgtgg cgctcacgcg cgcccaggag gccgctcatg cctgaggcga
     4261 tcatcgaggc gagagacctg gtgaagcgct tcgggcgctt cgtcgcggtc gaccacctct
     4321 cgctcaccgt gaaccggggc gagatcctcg gcttcctggg tccgaacggc tctggcaaga
     4381 gcacgacgat ccggatgctg tgcggactcc tccggcccac cagcgggagc gggagcgtga
     4441 tgggctacga tatcacccgc gaagccgagc agatcaaaga gcgcatcggc tacatgtcgc
     4501 agcgcttcag cctctatgag gacctgaccg tcgccgagaa tgtcctcttc tacgccggga
     4561 tctaccgggt gccccgcgcg cgccggaagg agcgcctgga gtgggtgctg gagatggccg
     4621 ggctgaagga gcaccgaaac cggctgaccg gcacgctctc cgttgggtgg aagcaacgcc
     4681 tggcactcgg gtgcgcgctg gcgcacgagc cggagatcct cttcctggac gagcccacga
     4741 gcggcgtcga ccccgtctcc cggcgtggtt tctgggatct gatcgccggg ctggctgagg
     4801 agggcgtcac ggtgatggtc acgacgcatg tgatggagga ggtcgagcac tgccaccgcg
     4861 ccgtgctgat ctaccagggg cggcaaatcg ccctgggtac cccggcggag ctgaaggcga
     4921 gcctgcccgg cgccctctac gaggtgcgcg tcgagcccgc gctcgccgcc ctgaaggcgc
     4981 tggagcgcgt cgaaggcgtt gaggacgcgg cactcttcgg cacggcgctg cacgtgcgca
     5041 cgcgaacgcc catcccagaa gcgatgctgg agaaggcgct cgtggatgcc ggttttcccg
     5101 gagcgcgggt cacgccggcg gaagttcagc tcgaggatct cttcatctcg ctcattgccg
     5161 aggcagaccg ctccgggaag gaggcgtagc gatgtggcgc cggctgtggg cgatctgccg
     5221 gaaagagtgg attcagatcc ggcgcgaccc gcgcaccctg gccgtcatct ggctgctccc
     5281 aatcacccag ctgctcctct tcggctacgg catggatttc gatctgaagc gggtccatgt
     5341 cgctgtcgcg gattacgacc gcagtcaggc gagccgcgtg ctggtcgccg ctttcgacca
     5401 gagcgagcac ttcgaggtga tcccggcggc cggacgcaca gcggaggtgg agcgcatggt
     5461 ggtggacgcc agggcgcgcc tgggtgtagt cattcccgcc ggtttcgagc gcgcgctgcg
     5521 tcttggccag cccgcgcccg ttcaggtgct cctcgacggc gccgatccga ccaccggcgg
     5581 caccgccctg ggctacacgc gcggcctgat cgagagcttc tcgcgcgagt ggttgcgaga
     5641 acacccgata cgcgcggttg cccccgccga gagcggttcc gatcaggccg tcttcgtctc
     5701 ctctcgccag ccggctgccc tggacgtgcg cgctcgcgtc ctctacaacc cggagctacg
     5761 cagcgccaac ttcctggtgc cagggctgat ggcggtgatc ctgacgatga tcgccgcgct
     5821 cctcacatcc aacgccgtgg cgcgcgagaa agagcggggc actttc
//
LOCUS       DAOSCV010000148         5852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8659_length_5852_cov_3.177161, whole genome shotgun sequence.
ACCESSION   DAOSCV010000148 DAOSCV010000000
VERSION     DAOSCV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8659_length_5852_cov_3.177161"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLY56_05075"
     CDS             complement(<1..1575)
                     /locus_tag="PLY56_05075"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ20886.1"
                     /translation="MEELPKAYEPGQVEGKWYQYWTERGYFHAEVDRSRPPFCITIPP
                     PNITGSLHMGHALCYTLQDILTRWRRMQGYNTLCLPGTDHAGIATQNVVEKGLAREGL
                     TRHDLGRERFLERVWQWREEYGNTIIRQFQTMGYSFDWDRLRFTLDDHYADAVLEQFV
                     RLYHEGHIYRGARVINWCPRCRTAISDIEVEHEEKAAHLYHIAYPFEDGEGSITVATT
                     RPETMLGDTGVAVHPEDDRYLGAIGRSLVLPLVDRRIPVVADEHVDPEFGTGAVKVTP
                     AHDPNDFEIGLRHRLPQVVVIGPDGAMTEDAGHYQGMDRFACRKQITEDLKAQGLLVR
                     VEDYTHSVGHCARCGTVLEPLLSEQWFMRMGELARPAIDAVKEGRIRFVPDRYARVYL
                     EWMEGIRDWCISRQLWWGHRIPVWTCPEGHYTVTKTAPEACAECGSRELTQDPDVLDT
                     WFSSALWPHATLGWPRETEELDYFYPTSVMVTARDILYLWVARMIMTGLKFVGDIPFR
                     DVYIYPTVQNWEGKRMS"
     gene            1794..2357
                     /locus_tag="PLY56_05080"
     CDS             1794..2357
                     /locus_tag="PLY56_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ20887.1"
                     /translation="MPELEAAKREEFKQLALPNLDALYRSAMRMTRNEADAEDLVQDA
                     LLRAYQFYDQFQQGTNFRAWLFKILTNTFINQYRRRVREPETLSYDESEDFILYNRLV
                     ESDEGEMGNPETVVLRKLQHEQVLAAIDRLPYDYRMVVTLADVEGLSYKEVADILGCP
                     IGTVRSRLNRARHMLQRNLWGYLQEAN"
     gene            2357..2602
                     /locus_tag="PLY56_05085"
     CDS             2357..2602
                     /locus_tag="PLY56_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ20888.1"
                     /translation="MNHSPDYETCVDIMHALGDYLSRELSPAEMEEVEEHLQWCELCM
                     NHYRFEKALTTHIRERAQALRAPETLRKRVLHLLDSA"
     gene            2710..4257
                     /locus_tag="PLY56_05090"
     CDS             2710..4257
                     /locus_tag="PLY56_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOJ20889.1"
                     /translation="MLRQRLRLAPALILFALALVGCGGSGNMATTGAIEGYVFGRASS
                     KAPSALAAPGRQTTPPEGTVPLNGALVTLEPGGRQVTTEVDRRGQGGYFRFLGLKPGS
                     YTVSVASDGYTAASFSVEVKAGATTLAGINNGQTILEPAGASPRRKWTVMVYMDGDGD
                     LEEYGILNMNQMETLGSDANINMVVQFDRGPGFDATNGDWQDTRRFLVTKDDNKQLIS
                     SPVLQNLGEVDMGTPEALTDFIKWTVARYPADRYVLVLWNHGAGWRSRAAASRGIIFD
                     DSSGSYLTMGELEQALEAGGTHFDLIAIDCSLMGMLEVFYEIKDHCDYITASEESPPG
                     PGYPYDTIMARLVANPNMSTEALGRVFVEEHLARYKQDAVTQALIQTSRLDAFAAKVN
                     AFAEALLPLVPLHRQQVDAARVGSQPYAYTYYRDLHDFAEKVKAAIPSPALASAADAV
                     MAGMAGTNGGPVIAEGHSGAAVDRSHGISVYLPSPTQYYTGYEELAFVKAHPKWGQLV
                     RAIANQQ"
     gene            5232..5687
                     /locus_tag="PLY56_05095"
     CDS             5232..5687
                     /locus_tag="PLY56_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ20890.1"
                     /translation="MQDVNLVIIVGEVSKAPVLRVKPSGLPKTEFTVEVERPSSPTSE
                     KRMTDMFLVDTWDNLAEWCISHLRKGSRVAVIGLLQREIFTNRDGEREHLTIIKAKFV
                     RLLDHPVDAQLPTMEAIRQDRWSPDMTADYLRIAGSLFFEKARSLETHR"
     gene            5671..>5852
                     /locus_tag="PLY56_05100"
     CDS             5671..>5852
                     /locus_tag="PLY56_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOJ20891.1"
                     /translation="MKLTDSDGCFACGANNPKGLHLSFRTIGEEHVAEFVPTPDYQGF
                     AGIVHGGILATLLDEA"
BASE COUNT         1139 a         1853 c         1797 g         1063 t
ORIGIN      
        1 gctcatccgc ttcccttccc agttctgcac cgtcggatag atgtagacat cacggaaggg
       61 aatgtctccc acgaacttca ggccggtcat gatcatccgc gctacccaca gatagaggat
      121 atcgcgcgcg gtgaccatca cgcttgtcgg gtagaaatag tccagctcct ccgtctcacg
      181 cggccagccg agcgtggcat gcggccacag cgcggatgag aaccaggtgt cgagcacatc
      241 gggatcctgg gtcagctcgc ggcttccgca ctcggcgcac gcctcgggag ccgtcttggt
      301 gaccgtatag tgcccctccg ggcaggtcca cacaggaatg cgatggcccc accagagctg
      361 gcgggagatg caccagtcac ggatgccctc catccattcg aggtacaccc gggcatagcg
      421 atccggcacg aagcggatcc ggccctcctt caccgcgtca atggcggggc gggccaattc
      481 gcccatgcgc atgaaccact gctcggagag gagcggctcg agaactgtgc cgcagcgcgc
      541 acaatggccc acggagtgcg tgtagtcctc cacacgcacg agaaggccct gcgctttcag
      601 atcctcggtg atctgcttgc ggcaggcaaa gcgatccatg ccctggtaat ggccggcatc
      661 ctcggtcatc gcaccatcgg gcccgatcac tacaacctgc ggcagccggt ggcgcaggcc
      721 gatttcaaag tcgttgggat cgtgcgcggg ggtcaccttg acggcgccgg tgccgaactc
      781 cgggtcgacg tgctcatctg cgacgacagg gatgcggcgg tccacgagcg ggaggaccag
      841 cgagcgcccg atcgcgccaa gatagcggtc atcctcggga tggacagcca cgccagtatc
      901 gccaagcatc gtctccgggc gcgtggtggc gacggtgatg ctgccttcgc catcctcgaa
      961 gggataggcg atgtgataga gatgggcagc cttctcctcg tgctccacct caatatcgga
     1021 gatcgcggtg cggcaacgcg ggcaccagtt gattacgcga gcgccccggt agatatgccc
     1081 ctcgtggtag aggcgcacaa actgctcgag cacggcgtcg gcatagtggt catcgagggt
     1141 gaagcggagc cggtcccagt cgaaggagta gcccatcgtc tggaactgac ggatgatggt
     1201 gttgccatac tcctctcgcc actgccagac gcgctcgagg aatcgctcgc ggcccaggtc
     1261 atgccgggtg agaccctcgc gggcaagccc tttctcaacg acgttctgcg tggcaatccc
     1321 cgcgtggtcg gtgccaggaa gacaaagggt gttgtagccc tgcatgcggc gccagcgcgt
     1381 caaaatatcc tgcaaagtgt agcaaagggc atggcccatg tgcagcgagc cagtgatgtt
     1441 gggtggcggg atcgtgatgc agaagggcgg ccgggagcgg tcgacctcag cgtggaaata
     1501 gccccgttcg gtccagtact ggtaccattt cccttccacc tggcccggtt cataggcctt
     1561 cggcagttcc tccatcaagt cgctcctttc gcgtgtatta taagggtacg tgcgcagact
     1621 gtcaatttat gcgcgccaac acgcgcctgc ccggcaagcg tgcgccgatc acggaatcgc
     1681 ggcggcatgc gcaacgcctt gacacgcctg gccgccatca cctacaatag gatgtgtggt
     1741 ggcaaggagc ctctcgccgc tgctccttca gaagctacgg agggatactt cgcatgcccg
     1801 aacttgaggc cgccaagagg gaggagttca agcaactcgc cctgcccaac ctcgacgcac
     1861 tctaccgctc tgcgatgcgg atgacccgca atgaagcaga cgccgaggac ctggtccagg
     1921 atgcgctcct gcgcgcttac cagttctacg accagtttca acagggaact aatttccgcg
     1981 cctggctctt taagatactg acgaacacgt tcatcaacca gtatcgccgt cgcgtgcgag
     2041 agccagagac tctcagctac gacgagagcg aggacttcat cctctacaac cgtctggtgg
     2101 agagcgatga aggggagatg ggtaacccgg agacggtggt cctgcggaaa ttgcagcacg
     2161 agcaggtcct cgctgcaatc gaccgcctgc cctacgacta ccgaatggtg gtgacgctgg
     2221 cggatgtgga gggcctctcc tataaagagg tcgccgacat cctcggttgc ccgattggca
     2281 cggtgcgctc tcggctcaac cgggcgcggc acatgctaca gcgcaacctg tggggttacc
     2341 tccaggaggc caactgatga accacagccc tgattacgaa acctgtgtgg atatcatgca
     2401 cgccctggga gattacctga gccgcgagct ctctcccgcg gagatggagg aggtagaaga
     2461 gcacctccag tggtgtgagt tgtgtatgaa tcactaccgc ttcgagaaag cactcacgac
     2521 ccacatccgc gagagggccc aggctctgcg cgcccctgaa accctacgca agcgcgtgct
     2581 tcacctcctc gacagcgcct gatccggcat acttcttgcc gtttgggtgg cgcctgattg
     2641 aaccttcggc gtcacccgac cgtcccatag agtgtgtgtc tgcattccga tagcatggaa
     2701 gggaatggca tgcttcgtca acggctccgg ttagctcccg cgctcatcct gtttgcactg
     2761 gcgctcgtcg gatgcggcgg atccggcaac atggcgacca cgggcgctat cgaagggtat
     2821 gtgttcggtc gagcctcctc gaaagctcct tccgcccttg cggcgcccgg ccgtcagaca
     2881 accccacccg agggcaccgt cccgctcaat ggggcgctgg tgacgctgga gcccggcggt
     2941 cgccaggtga cgacagaggt agaccgccgc ggccagggcg gctacttccg cttcctcggt
     3001 ctgaaacccg gctcctacac cgtgtccgtg gcgagcgacg gctacaccgc cgcatccttc
     3061 tccgtcgagg tgaaggccgg cgccaccacc ctcgctggca tcaacaacgg acagaccatc
     3121 ctggagccgg ccggcgccag cccgcgccgc aagtggacgg tgatggtcta catggatggc
     3181 gacggtgacc tcgaggagta cggcatcctc aatatgaacc agatggagac gctgggctcc
     3241 gacgccaaca tcaacatggt cgtccagttt gaccgcgggc ccggcttcga tgccacgaat
     3301 ggcgattggc aagatacccg gcgcttcctg gtgaccaaag atgataacaa gcagctgatc
     3361 tcctcgcccg tgctccagaa cctgggcgag gtggacatgg gcaccccgga ggccctgact
     3421 gatttcatca aatggacggt agcacgctat ccggccgatc gctatgtgtt ggtcctctgg
     3481 aaccatggcg cgggctggcg ctcgcgcgct gccgcctcgc gcgggatcat cttcgacgac
     3541 tcctctggta gctacctgac gatgggagag ctggagcaag cgctcgaggc cggaggcacg
     3601 catttcgacc tgatcgccat cgactgcagc ctgatgggga tgctcgaagt cttctacgag
     3661 atcaaggacc actgcgacta tatcacggcg tctgaagagt cgccccctgg gccgggctat
     3721 ccctacgaca cgatcatggc acgcctagtc gccaacccga acatgagcac cgaggcactg
     3781 ggacgcgttt tcgtcgagga gcacctcgcg cgctacaagc aagacgctgt tacccaggcg
     3841 ctcattcaaa cctcgcggct cgacgcattt gccgccaaag tgaacgcctt cgccgaggcg
     3901 ctcctcccgc tggtgcctct ccaccgtcag caggtcgatg cggcgcgtgt cggctcacaa
     3961 ccctacgcct acacctacta ccgcgatctg cacgatttcg cagagaaggt gaaggccgcc
     4021 attcccagtc cagcgctagc cagcgccgcc gatgccgtga tggccggcat ggcgggaacc
     4081 aatggcggac cggtgattgc tgaggggcat tcgggcgcgg ccgtggaccg ctcgcacggg
     4141 atctcggtct acctcccctc gcctacccag tactacaccg ggtatgagga gctggcgttc
     4201 gtgaaggccc atccgaagtg gggccagttg gttcgggcga tcgccaacca gcagtagacc
     4261 cacgacggag acaaggatcg aacagggggt gcccttgcgt ggcaccccct gttgctttct
     4321 gtgtgctacc gccccccgag gatcggttgc tgcgcacccg ccggcccctc acctccagaa
     4381 agggccgctc gccatgcaaa cggacagata atgtcctact caagcactct ggctgcttca
     4441 gcctgcttgc cagagttgac agcggtgacc aggatgcggt atgataggat acgatgccag
     4501 atgctggcgg cagcctggcg ggcgtcagga agcaggaaag cgggttacgg agttctgaag
     4561 cagcgcgcct accttcaacc gggtggacaa gcagagacga gggcgcccgc ggtgagcgca
     4621 tcctggcgag agaaccagcg gcaaaccatg cacaagtctc tcctttcttg gcacgatgtc
     4681 gacccagatt gcatccgttg atctgcccgg gtctactggg ctcgggccga cgacagcgag
     4741 tgcgtatcgc gctttagccc ggccggagct tccggtcgat acacatatcc atcgcttatc
     4801 acagcgtctc tggttaccga agcagcgcat gggcgcggcc ggctatgtgc cgtgcacggt
     4861 tggtttctgc acaacgagta cgtccgctca tttcacagga cgagagtgcc ttcggctagg
     4921 gcatctgccc ggcgcatctg tcgcgcattg gaggacacgg gcagccttgt aaggttattg
     4981 caggagaagc tttgcgcatg gcgcggccag gccacagagc gcgatgcctc gatggctacc
     5041 gccccgcgcg aacaaagtcg ccaggcacat gctgagaagc cggcggcgat ggttcccact
     5101 tccatcctcg gcagaccagg agtgcagtgg atggggcgtc acacccatct cagtccattc
     5161 atggggcaaa cgctctcatg aagtgtcggg ctgattgcat catcggggtg tgccgccagg
     5221 ggggtttagg catgcaggat gtcaatctgg taatcatcgt gggggaggtc tccaaggcac
     5281 ccgttctgcg ggtgaaacca tccgggctgc ccaagacaga gtttacggtg gaggtggagc
     5341 gccccagctc gccgaccagc gagaaacgaa tgacggacat gttcctcgtg gacacctggg
     5401 acaacctggc ggagtggtgc atcagccacc tgcgcaaggg ctcccgggtc gcggtcatcg
     5461 gcttgcttca gcgcgaaatc ttcacgaacc gtgatggaga acgcgagcac ctcaccatca
     5521 tcaaggcgaa gttcgttcgc ctcctcgacc atcccgtcga tgcccaactt ccgaccatgg
     5581 aagcgattcg gcaggaccgc tggtccccag acatgaccgc cgattatctc cggattgctg
     5641 gctctctctt cttcgagaag gcacgctctc ttgaaactca ccgatagcga cggctgtttc
     5701 gcctgcggcg ccaacaatcc caaaggcctg cacctgagct ttcgcaccat cggcgaagag
     5761 cacgtcgccg agttcgtgcc aacgcccgat taccagggct tcgccggcat cgtgcatggg
     5821 ggcatcctcg cgacgctgct cgatgaagcg at
//
LOCUS       DAOSCV010000149         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8661_length_5851_cov_3.607660, whole genome shotgun sequence.
ACCESSION   DAOSCV010000149 DAOSCV010000000
VERSION     DAOSCV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8661_length_5851_cov_3.607660"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            629..2323
                     /locus_tag="PLY56_05105"
     CDS             629..2323
                     /locus_tag="PLY56_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ20892.1"
                     /translation="MKRWIIALIPLLVLGWLINGRLQEKRAEARMASEQRQARRQMMP
                     VVSLAPAQVRDIVRSFEGVGSVEAPLSVKVSSQITGRITFIQVREGDPVRQGQVLVRI
                     DPSELRAQVAQRRAAVAEAKHRLAQAAENEAPTEVSVTADIRQQEAALASAQARAAQA
                     EQHLDAEVAAAKAAVTEAEGRVESAMASMANAEAAIRSAQANLDNARAAFKRVNDLYQ
                     QGYVAAQDVDNARTQVSVQEAALDAARGQLKAAIAARDSAIAQKQAAEKRVEITRTRC
                     TADVQAARAQVSQARAALEFARANTARKPAYQRNLSALQAAVDAAQASLENAEAMLAH
                     TVITSPIDGFVTARNLDPGAVATPGQQILAVQAMNQVWVTISVPEEVHRQVRKGDTAT
                     VSFDALPGKSFTGKVTQINAAADPVSRQFAIRITLDNRDNLLKPGMFARVTLVTERVP
                     NAVVVPPEAVQTGKNGPFVVVVDAENIARHRPVVTGASDNQGLEIREGLQPGERIVVM
                     TARPLKDGQAVRPAGEGSKQGAGKRPDEERGAPRQSGSGQGPASAAGPGSGGQRPE"
     gene            2320..>4351
                     /locus_tag="PLY56_05110"
     CDS             2320..>4351
                     /locus_tag="PLY56_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ20893.1"
                     /translation="MNIAKFSVSRPVTVTMRIAAMVLLGAICLTRLPVDLLPEVSLPS
                     ISVVTSWPNVAPEEIEAQITRPIERAVSTLSNVYEVSSTSSEGNSSVRIQFNWGTDIG
                     QAAVDVLQQVQRAQRSFPNDPTLQNPTVYKFDPSQMPILTIAVTGIADQVKLRTLLDN
                     EISPIIEAADGVASVAVTGGQERVILVEVDPARLRAHNLTLSQITSRLVQENANLPAG
                     IARQSDTEYLIRSLGWFTDPKEIERLPVGSFNGRLVTLRDVATVSDTHAETRMYSRLN
                     GEPAAGLIVSKQSGANTVNTAKAVFKRVERARELYPELSFHVASDQSHFIEHAVADVK
                     THAIIGGILAVLILLFFLRNVRSTLVVALSIPVSVISTFALLYLCGYTLNTMSLGGLA
                     LAVGLIVDDAVVVLENIFRHIERDGKSAEEAAVTGTQEILSAVVASTLTVIIVFLPLL
                     LIKGQAGQMFAQFALVVVFAISLSLVDATMSVPMFASRLIRGEAHRENLSNGHRPGLL
                     ERAFAAAGRFFDSLDTTYRGALYWALRHRFWVIAIAGAVTAATWLLTPFIGTEMMPET
                     DTGDLTVRINMPVGTALDKTDAVVRKAEEIIMRHPDVEMILASAGTGMALRGTSPRLQ
                     GNEGSVTVKLRADRKRSSAEVIGELRKELSQISGARIQIRSTDLVSRIITGGA"
     assembly_gap    4352..4361
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4362..5523
                     /locus_tag="PLY56_05115"
     CDS             <4362..5523
                     /locus_tag="PLY56_05115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ20894.1"
                     /translation="ATLSSLARTMMDRMREIPGLENLDVNWQEATPEVQWRVDRDKAL
                     QLGVSFTDVANTLKTATSGTIASYFQEDGFQYPILVQLPESKRKNVDDLMNIPLALAG
                     SGGASGEGAQHVLLGQVAQPEFAVGPSDITRLNRRRYISVSGSPQGRPLSEIRADVEK
                     ALADVQMPAGYYRDWGTSMKRQQEEFAGMGLAVILAVLLIYMLLAAQFESFVHPLTIL
                     ASVPLSAIGVALALFLTGRSFGLTAFIGLLMLVGIVVKNGILLIDYTNILRHRGMDRD
                     TAVLTAGPTRLRPILMTSSASILGMLPIATGLGQGMEMQAPMATAVIGGLTTSTFLTL
                     LVVPVVYTLFDDLANLGGRGKRRRAKAASHAEGNGHAQAEATVVTARPDAAS"
     gene            5544..>5851
                     /locus_tag="PLY56_05120"
     CDS             5544..>5851
                     /locus_tag="PLY56_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20895.1"
                     /translation="MPDDTMSNRTVTNTLTRSLLTLALAGAASGTAWAQLTPPISPKT
                     RETPLPPAVTLPAPAEIPEELRERSLTAADAARIALRQHNTVAAARASITQLEGRTRQ
                     A"
BASE COUNT         1026 a         1995 c         1860 g          960 t
ORIGIN      
        1 gtccaaccca gcccggcagg gcttcgctcg caagcagcgc ggtgatttat cgccgtgcgc
       61 cgggttgcgc gctgcctccg aggcttcgga ttccttatgt cggaatcccg gcacattgcc
      121 ccacgctctt tacctgttgg cactcatggg gtatactggc ggtagtgtga gcacctgagg
      181 tggaaaatca ggcaccatta ccccgcgagg ggattgatgc cggggagcct gtccggccag
      241 gtgttcactc ctcattgcct caagagaacc ccgttctcga tgccggtttc acgcgttcgg
      301 atcaccttgc tgctctccag gccggtcctg ccccgttccc ctgcccgccc gggaccagaa
      361 tgttagcgtt ctgttaacac tgacccgcag gcaaggatcc ggcgtcaaag atagcaaaca
      421 ggaagtaatg gtcgagcctg aacctgaggc ggaggtcgag gtaatctccc cttggcgtcc
      481 gccgccctcg gtgtgcaagg cgtcggaatc ctacacaccc ggcgcgaggg gcaatgcgcc
      541 aggccatcta ttccccggcg ctcgttccgc tagtctccct ggaagcaccc aggcgcgctc
      601 ttggccggcc tcgagggatc gcacgaatat gaagcgttgg atcattgcac tcatcccact
      661 ccttgtgctg gggtggctga tcaacgggcg actgcaggag aagcgggccg aggcccgcat
      721 ggccagcgag cagcggcagg cccgccggca gatgatgccg gtcgtctcgc tagcccccgc
      781 gcaggtgcgc gatattgtcc ggtcgtttga gggggtaggc agcgtcgaag cccctttgag
      841 cgtcaaggtt tcttcacaaa tcacggggcg gatcacgttc atccaggtac gggagggcga
      901 tccggtccgc cagggccagg tgttggtgcg catcgatccc tcggagctgc gcgcccaggt
      961 ggcgcagcgg cgggcggcgg tcgccgaggc caagcaccgc ctggcgcagg ccgccgagaa
     1021 tgaggcgccg acggaggtct cggtcaccgc ggatatccgc cagcaagagg cggctctcgc
     1081 cagcgcccag gcgcgcgccg ctcaggcaga gcagcacctg gatgccgagg tcgccgcagc
     1141 gaaggcagcc gtcaccgagg ccgagggccg ggtagagtcc gcaatggcct cgatggcgaa
     1201 tgctgaggcc gcgatccgga gcgctcaggc caatctagac aacgcgcgtg ctgccttcaa
     1261 gcgcgtcaat gacctctacc agcagggcta cgtcgccgcg caggatgtcg acaacgcgcg
     1321 cacgcaggtg agcgtccagg aggcggcgct cgatgccgcg cgcggccagc tcaaggccgc
     1381 catcgcggcg cgcgattcgg ccatcgccca gaagcaggct gcggagaagc gcgtggagat
     1441 cacgcgtacc cggtgcaccg ccgacgttca agccgcgcgc gctcaggtct cccaggcacg
     1501 cgccgcactg gagttcgccc gcgccaacac agcccgcaag ccggcctacc agcgaaacct
     1561 ttctgctttg caggccgccg tcgatgccgc gcaggcttcc ctagagaacg ccgaggcaat
     1621 gctcgcgcac acggtgatca catcccccat cgatggcttt gtcaccgcgc gcaacctgga
     1681 cccgggcgcg gtcgccactc ctggccagca gattctggcg gtgcaggcga tgaaccaggt
     1741 atgggtcacc atctcggtcc ccgaggaggt gcaccggcag gtgcgtaagg gcgatacggc
     1801 cacagtgagc tttgatgcgc ttccggggaa gagcttcacc ggcaaggtga cgcagatcaa
     1861 tgccgctgcc gaccctgtct cgcgccagtt cgcgatccgc atcaccctgg ataaccgcga
     1921 taacttgctg aagccgggca tgttcgcgcg ggtcacgctc gtcacggaac gggttcccaa
     1981 cgcggtcgtc gtgccgccgg aggccgtgca gaccggaaag aacggcccat tcgtggtggt
     2041 cgtcgacgct gagaacatcg cccgccatcg accggtggtg accggcgcga gtgacaatca
     2101 aggcctcgag atccgcgaag gattgcagcc cggcgagagg atcgtcgtga tgacggcccg
     2161 cccgctcaaa gacggccagg ccgtgcgccc cgcgggtgaa gggagcaaac aaggcgcagg
     2221 caagcgcccc gacgaagaac ggggcgcccc acgccagtct ggcagtggtc aaggcccggc
     2281 gtctgccgcg ggccctggct ccggagggca gcggccggaa tgaacatcgc gaaattctcc
     2341 gtctctcgcc cggtgaccgt caccatgcgg atcgcggcga tggtcctcct gggcgccatc
     2401 tgcctcacgc gcctgccggt ggatcttctg ccggaggtat cgcttcccag catctcggtc
     2461 gttacctcct ggcccaacgt ggcgccggag gagatcgagg cgcagatcac ccgcccgatc
     2521 gagcgcgcgg tctccacgct cagcaacgtc tatgaagtga gctccacatc ctcggagggc
     2581 aactcgtccg tgcgcatcca gttcaactgg ggcaccgata tcgggcaggc cgccgtggac
     2641 gtgctccagc aggtgcagcg cgcccaacgc tccttcccca acgacccgac gctgcaaaac
     2701 ccgacggtct acaagttcga cccgtcgcag atgccaattc tgacgattgc agtgaccggg
     2761 atcgccgacc aggtgaagct gcgcaccctc ctggataacg agatctcgcc gatcatcgag
     2821 gccgccgatg gcgtcgcctc ggtggcggtg acgggcggcc aggagcgagt gatcctggtg
     2881 gaggtggatc cggcgcgcct gcgcgcgcat aacctcactc tctcgcagat cacgagccgc
     2941 ctcgtccagg aaaacgccaa cctccctgcc ggcattgccc ggcaaagcga tactgagtac
     3001 ctcatccgta gcctggggtg gttcaccgat ccgaaagaga tcgagcgcct tccggtgggc
     3061 tccttcaatg ggcgcctggt cactctccgc gacgtggcga ccgtctccga cacccacgcg
     3121 gagacacgca tgtactcgcg cctcaatggg gagccggccg ccggcctgat cgtctccaag
     3181 cagagcggcg ccaacaccgt gaacacggcc aaagcggtgt tcaagcgtgt ggagagagca
     3241 cgcgagctct atccggagct cagctttcat gtcgcgtccg accaatcgca cttcatcgag
     3301 cacgccgtcg ccgacgtgaa gacgcacgcg atcatcggcg gcatcctcgc cgtcctgatc
     3361 ctcctcttct tcctgcgcaa cgtgcgcagc acgctggtgg tggcgctctc catcccggtt
     3421 tcggttatct ccacgttcgc gctcctctac ctgtgcggct atacgctgaa tacgatgtcg
     3481 ctgggcggcc tggcgctcgc agtcggcctg attgtggatg atgccgtcgt cgtcctcgag
     3541 aatatcttcc ggcatatcga aagagatggc aaaagcgccg aggaggccgc tgtcaccggc
     3601 acgcaggaga tcctatcggc cgtcgttgcc tcgacactga ccgtcatcat cgtctttctg
     3661 ccgctgctcc tgatcaaggg gcaggccggc cagatgttcg cgcagttcgc gctggtggtt
     3721 gtcttcgcga tctcgctctc gctcgtggat gccaccatgt ccgtcccaat gttcgcatcc
     3781 cgcctcattc gtggcgaggc gcatcgcgag aacctctcca acggccaccg gccggggctc
     3841 ctggagcgcg cgttcgcggc cgcgggccgc ttcttcgatt cgctggatac gacctaccgg
     3901 ggcgcgctct actgggctct tcgccatcgt ttctgggtga tcgcgattgc tggcgcggtg
     3961 acagcggcca cgtggctgct cacgccgttc atcggcacag agatgatgcc cgagactgat
     4021 acgggcgacc tgaccgtcag aatcaacatg cccgtcggaa ccgcgctgga caagacagat
     4081 gccgtcgtgc gcaaagccga ggagatcatc atgcggcacc cggatgtcga gatgatcctc
     4141 gcctcggcgg gcacgggcat ggcgttgcgc ggcacatccc ccaggcttca aggcaacgag
     4201 ggctcggtga ccgtaaagct gcgcgcggat cgcaagcgct cctcggcaga ggtgatcggc
     4261 gagctgcgga aagagctctc ccagatctcg ggcgcgcgca tccagatccg ctccaccgac
     4321 ctggtgtcgc gcatcatcac cggaggcgcg cnnnnnnnnn nggccaccct ctccagcctg
     4381 gcccggacca tgatggaccg catgcgcgag atcccgggac tggagaacct ggacgtaaac
     4441 tggcaggaag ccaccccgga ggtccagtgg cgcgtggatc gcgataaggc gctccagctg
     4501 ggcgtctcct tcacggacgt tgccaacact ctgaagacgg cgactagcgg caccatcgcg
     4561 agctacttcc aggaggatgg cttccagtat cccatcctcg tccagctgcc cgagtcgaag
     4621 cgcaagaacg tggatgacct gatgaacatc cccctcgcgc tcgcgggatc gggaggggca
     4681 tccggcgaag gagcccagca tgtcctcctg gggcaggtgg cgcaaccgga gtttgcggtc
     4741 ggccccagcg acattacacg gctcaaccgt cggcgctaca tctccgtctc cggctccccc
     4801 cagggccggc cactcagcga gatccgcgcg gatgtagaga aagcactcgc ggatgtacag
     4861 atgcccgccg gctactatcg ggactgggga acgagcatga agcgccagca agaggagttc
     4921 gcggggatgg gcctggccgt gatactcgcg gtgttgctca tctacatgct cctcgccgcg
     4981 cagttcgagt cgttcgtgca tcccctgacg atcctcgcct ccgttccgct ctcggcaatc
     5041 ggcgtcgcac tcgcgctctt cctgacgggt cggtcattcg ggctgaccgc gttcatcggc
     5101 ctgctgatgc tggtgggaat cgtggtcaag aacggcattc tgctgatcga ctacacgaac
     5161 atcctgcggc atcgcggcat ggatcgcgac accgccgtgc tcacagccgg accgacgcgc
     5221 ttgcgaccga tcctgatgac gagctccgcg tcgatcctcg gcatgctgcc aatcgccacc
     5281 ggactgggcc agggcatgga gatgcaggcg cccatggcga ccgccgtgat cgggggtctc
     5341 acgacctcca ccttcctcac gctcctcgtg gtgcccgtgg tctacaccct ctttgatgac
     5401 ctggccaacc tgggaggccg gggtaagcgc cggcgagcca aagccgcaag ccacgcggag
     5461 ggcaacggcc acgcccaagc ggaggcgacc gtggtcacag cacgccctga cgccgcatcc
     5521 tgacacgaac cgctgctgga gagatgcctg acgataccat gtccaatcga acagtgacca
     5581 acaccctgac acggagcctg ctgacgctgg ccctcgccgg cgccgcgtcg ggcacggcct
     5641 gggcgcagct cacgcccccc atctcgccaa agacgcgcga gacgccgctg ccccccgcgg
     5701 tcacgctccc cgcgccagcc gagatcccgg aggagctgcg cgagcgctcg ctgaccgcgg
     5761 ccgatgccgc acggatcgcc ctgcgccagc acaacaccgt tgccgccgct cgcgccagca
     5821 tcacgcagct ggaggggaga acccgccagg c
//
LOCUS       DAOSCV010000150         5840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8693_length_5840_cov_3.664996, whole genome shotgun sequence.
ACCESSION   DAOSCV010000150 DAOSCV010000000
VERSION     DAOSCV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8693_length_5840_cov_3.664996"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PLY56_05125"
     CDS             <1..1309
                     /locus_tag="PLY56_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20896.1"
                     /translation="PRGHRHLRGAPSGAEGALVPVTPERPGSVVAAGPALAFFWHSGH
                     PGVGRILSEWACAWAADILSHVDPEEPGRLPAAIDPATCGRLDTGARPSAREKNLLRR
                     FLLDIHRVSGQKDLLRPVALAVAAGDADPVTAALWRAQGHAEHDEQIARDARGADDLR
                     AYAAWIATRNKQFVVDGLQHALERLENERYLATEANPPPEALEIPGDLLLRSLYLGGC
                     ATWQGGMPRVAVTWENIGVDFAAFVTESRTDALKVLLYHFRPEARPVQMRLWALRPGQ
                     YQLEVGPDDDYDDEPDRVTERRVVEVERGVALPVMLPAQRPYVLRLVAKSLRPLAAAP
                     DVALCADEVALSPDRRRVSVVVHNFGTAPAIKVRLTVTDTRRRVIGSGAIASLSWPEN
                     LKTAAARVEIPLVTPAPEAFRVTATLASPELSTANNSVAVYAR"
     gene            complement(1320..2072)
                     /locus_tag="PLY56_05130"
     CDS             complement(1320..2072)
                     /locus_tag="PLY56_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ20897.1"
                     /translation="MTGASTGIGRQIAIELGKRGAAVAIHYHANVAGAGETARAIQES
                     RGRAFLIQANLASPQECTRLIAACASVLGGIDLLINNAALSPEAPFLDLSDEPWERTL
                     PVGLRAPVLCAQAAARQMIRQGRGGRIIYVGSAHGFATASHTGPYTASRGALHMLTKQ
                     MARELAPHRIAVNCVAPGPSETKHEETPFPEYDPELPTHPLLWERAGIPVNIARAVAF
                     LCSDEAGFLTGQVIRVESGEPPGQWKARGASG"
     gene            2273..4384
                     /locus_tag="PLY56_05135"
     CDS             2273..4384
                     /locus_tag="PLY56_05135"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOJ20898.1"
                     /translation="METREQGELSQEALLARLDALGEWFAAGFLSLPGASPARRMAAG
                     IRRYLEHAPLPEYTGRRLYPHGPTIWQTGNAVYHHYVQMEYARAIYERKRAAAATDED
                     RAALDAMDAFFSSYPYAAGYTHSIPNYRRILNEGLDAYRERVEKHRALSEKRGEAARA
                     EWYEAFGEVLDAVAGLRRRCIEKIQAAALESPGAEQNREALLAALARVPFAPARSFTE
                     AMVAENFLFYLDGCDDLGRFDQDLLPYYERSRPSDEEALALIGELWENVDDACAWNVA
                     LGGTTRDGDPAYNPLTLLALRAGKGRRRPNLALRLRRDTPEAVWDTALETLSGGTGIP
                     ALYAEENYLAALEEAHLNVSRADLHDFAFGGCTETMIAGCSNVGSLDDKINLPEILVN
                     ALHAELPACASFDAFLERIRPYYIRQIREITDRVSRYQERRARHHPQLIRSLLIDDCI
                     DNGRDYAAGGARYNWSVINVEGLGNVIDSLAAIREVVYERREVTPERLLSALRDNWEG
                     DEALRRRVQRCPRYGNGDPAVDALAREFSEFIFREFLRYAPYRGGKFLPSCLMFVTYA
                     DEGQRVAATPDGRRAGEPIADSAGAVQGRDRSGPTAALQSAASLDTRHAPGTLVVNIR
                     LSKQLFADREKVKALVRGYFALGGLQVQINVVDQAVLRDALEHPEQHGDLIIRVGGYS
                     EYWTRLSRPLQESVLQRVEHV"
     gene            4397..4915
                     /locus_tag="PLY56_05140"
     CDS             4397..4915
                     /locus_tag="PLY56_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ20899.1"
                     /translation="MGGTARIIGRIFDIQRFSVHDGPGIRTTVFFQGCPLRCRWCHNP
                     EALSYGPALFYNDRSCFFCRACEAACTEAVHSFVGEEHRMHRERCRLCGACAAACVTG
                     ALEISGRTVTPEEVVEVVLRDRVFYETSGGGVTLSGGEPLAQPAFAGEILARCRAEGL
                     HTAVDTCGEGAR"
     gene            4961..5350
                     /locus_tag="PLY56_05145"
     CDS             4961..5350
                     /locus_tag="PLY56_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20900.1"
                     /translation="MKHLDPEAHRAATGRSNERILQNLRYLDSVLCREGGPAPAGARL
                     VLRVPLIPRINDTPENLAALAALARELPSAREMRLLPYHRFQEDKYRRLGREYPGTDL
                     VPPDDATVKAIRERLAAEAGIIVGIGG"
BASE COUNT          873 a         1927 c         2066 g          974 t
ORIGIN      
        1 cccccgcggc caccgtcacc ttcggggcgc tccctcgggt gcggaaggcg cgctggtgcc
       61 ggtgacgccc gagcggccag ggagtgtcgt cgccgccggg ccggcgcttg ccttcttctg
      121 gcattcgggt catcccgggg tgggccgaat cctgagcgag tgggcctgcg cttgggcggc
      181 ggacattttg agccacgtgg atccggagga gccgggccgg cttccggccg cgattgatcc
      241 ggctacatgc gggcgcctcg acaccggggc ccgcccgagt gcccgggaga agaacctcct
      301 gcgacgcttc ctcctggaca tccatcgcgt ctctggccag aaggatctgc tgcgtccggt
      361 ggcgctggcg gtcgccgcgg gcgatgccga tccggtgacc gccgcgctct ggcgcgctca
      421 gggtcacgcc gagcacgacg agcagatcgc aagggatgcc cgaggcgcgg acgacctgcg
      481 tgcctacgct gcctggatcg ccacccggaa caagcagttc gtggtggatg ggctgcagca
      541 cgccctggaa cgcctggaga atgagcgtta cctggccacg gaggcgaatc caccccccga
      601 ggcgctcgag ataccgggag acctgttgct ccgctcgctc tacctgggcg ggtgcgccac
      661 ctggcagggc ggcatgccgc gcgtcgcggt gacctgggag aatatagggg tagattttgc
      721 cgccttcgtc accgaaagcc gcactgacgc gctgaaggtg ctcctctacc atttccggcc
      781 ggaggcgcgg ccggtgcaga tgcgtctctg ggcgctccgc cccgggcaat accagctgga
      841 agtcgggccc gatgacgatt acgacgatga gccggaccgc gtgacggagc gccgtgtggt
      901 ggaagtggag cgcggagtgg cgcttcctgt gatgctcccg gcgcagcggc cctacgtcct
      961 ccgactcgtg gcgaagtcgc ttcgcccgct cgcggccgcc cctgatgttg ccctctgcgc
     1021 cgatgaggtc gcgctttcgc ctgaccggcg gcgcgtgagc gtcgtggtcc acaacttcgg
     1081 cacggccccg gccatcaagg tacgcctgac cgtcacagac acgcgccggc gggtgatcgg
     1141 ctcgggcgcc atcgcgagtc tctcgtggcc cgagaacctg aagaccgcgg cggcgcgcgt
     1201 ggagataccg ctcgtcacgc ccgcgccgga ggcgttccgg gtcaccgcga cgctcgcatc
     1261 cccggagctc tccaccgcga acaacagcgt ggcggtttac gcgcgctagg gttgaggggc
     1321 tacccggaag cgccgcgcgc tttccactgg ccgggcggct cgccgctctc cacgcggatc
     1381 acctgcccgg tcaggaagcc cgcctcgtcg gaacagagga aggcaaccgc gcgggcgata
     1441 ttcactggga tgccggcgcg ctcccagaga agcgggtgcg tcgggagctc ggggtcgtac
     1501 tcggggaaag gcgtctcttc gtgcttcgtc tcggacgggc ccggcgcgac gcagttgacg
     1561 gcgatgcgat ggggcgccag ctcgcgcgcc atctgcttcg tcagcatgtg taacgcgcct
     1621 ctcgaggcgg tgtagggtcc ggtgtgagac gccgtcgcga agccgtgggc ggacccgacg
     1681 tagatgatcc tgccgccgcg cccctggcgg atcatctggc gcgctgcggc ctgggcgcag
     1741 aggaccggcg cgcgcaggcc gacaggcaga gtgcgttccc acggctcgtc ggaaaggtcg
     1801 aggaagggcg cctcggggct gagcgcggcg ttgttgatga ggaggtcgat cccccccaag
     1861 acgctcgcac acgcggcgat caggcgcgtg cactcctggg gactcgccag gttcgcctgg
     1921 atcaggaagg cgcgtccgcg actctcctgg atcgcccggg ccgtctcgcc ggcgcctgct
     1981 acattggcgt ggtagtggat cgccaccgcc gccccgcgct tccccagctc gatggcgatc
     2041 tgccgcccga tcccggtgct tgcgcctgtc accgcggctc tcttgccggc gagtggaccc
     2101 atccgcttcc ccctatctct gtctctcccc gggggcctct ttcggcgcga gagggatgcc
     2161 tcctgccggg caggcatccg gcgtccggcc acgtgggtga tgcccgcggg ccggaagcgg
     2221 gaacttctga gggctggggg agtctgactc gtgaaggaga cggaggagcg agatggagac
     2281 gcgggagcag ggcgagctgt ctcaggaggc gctcctggcg cggctcgacg cgctgggaga
     2341 gtggttcgcg gcagggtttc tgtcgctgcc aggcgcttcg ccggcgcggc ggatggcggc
     2401 gggcatccgg cgctatctcg aacacgcgcc gttgccggag tacaccggcc ggcgcctcta
     2461 cccccacgga ccgaccatct ggcaaaccgg caacgccgtc taccatcact atgttcagat
     2521 ggagtacgcg cgcgcgatct acgagcgcaa gcgcgcggcg gcagcgacgg acgaggatcg
     2581 cgccgccctc gacgcgatgg atgccttctt ctcaagctac ccctacgccg cgggctacac
     2641 gcacagcatc cccaactacc gtcgtatcct gaacgagggc ctggacgcgt atcgtgagcg
     2701 cgtggagaag catcgggcgc tcagcgagaa gcgaggcgaa gctgctcgtg cggagtggta
     2761 cgaggcgttc ggagaggtgc tggacgcggt ggcgggcctg cgccggcggt gcatcgagaa
     2821 gatccaggcg gcggcgctcg aatcgccggg cgcggagcag aatcgggagg cgctcctcgc
     2881 tgcactggcg cgtgtgccct tcgcgcccgc ccggagcttc accgaggcga tggtcgcaga
     2941 gaacttcctc ttctacctgg atggctgcga cgacctggga cggtttgatc aggacctgct
     3001 gccctactac gagcgaagcc ggccttcgga tgaggaggcg ctcgcgctga tcggcgagct
     3061 gtgggagaat gtggacgatg cctgcgcctg gaacgtggcc ctgggtggca ccacccggga
     3121 tggcgatccc gcctacaacc cgttgacgct gctcgcgctt cgcgccggca aggggcggcg
     3181 gcgccccaat ctagcgttgc gcctgcgccg cgataccccc gaggcggtgt gggacacggc
     3241 gctcgagacc ctctccggcg gcaccgggat cccggcgctc tatgccgagg agaactacct
     3301 cgcggcgctg gaagaggccc acctcaacgt atcccgcgcc gacctccatg acttcgcctt
     3361 cggcgggtgt acggagacga tgatcgccgg ctgttccaac gtcggctcgc tcgatgacaa
     3421 gatcaacctt cctgagattc tggtgaacgc gctgcacgcc gaactgcccg cctgcgccag
     3481 cttcgacgcg ttcctggagc gcatccgtcc gtactacatc aggcagattc gcgagatcac
     3541 cgaccgcgtc agccggtacc aggagcgtcg cgcccggcac cacccccagc ttatccggtc
     3601 gctcttgatt gacgactgta tcgataacgg ccgagactac gcggccgggg gcgcacgcta
     3661 taactggagc gtgatcaacg tcgaaggcct ggggaatgtg atcgattcac tcgccgcgat
     3721 tcgggaggtc gtttacgagc gccgcgaggt gacgccggag cgcctgctga gcgcgctgcg
     3781 cgataactgg gaaggcgacg aggcgctgcg ccgacgcgtg cagcgctgcc cccgttacgg
     3841 gaatggcgac cctgcggtgg atgcgttggc gcgggagttc tcggagttca tcttccgcga
     3901 gttcctccgc tacgcgccct atcggggcgg gaagtttctc ccctcctgcc tgatgttcgt
     3961 cacctacgcc gatgagggcc agcgtgtggc cgcaaccccg gatggtcgcc gggccggcga
     4021 gccgattgcc gactctgccg gcgcggtgca gggccgcgac cggagcgggc cgacggcggc
     4081 gctgcagtcg gcggcatcgc tcgatacgcg gcacgctcct ggcacgctcg tggtgaacat
     4141 ccggctttcg aagcagcttt ttgccgaccg cgagaaagtg aaagcactcg tgcggggcta
     4201 ctttgccctg ggcggtctcc aggtgcagat caacgtggtg gaccaggcgg tcctgcgcga
     4261 tgccctggag cacccggagc agcatggcga tctgatcatc cgcgtcggtg gctattcgga
     4321 gtattggacg cgcctctcac gccccctcca ggagagcgtg ctgcagcgcg tggagcatgt
     4381 gtagaaaggg ttgccggtgg gcggtacggc gcggatcata ggcaggatct tcgacattca
     4441 acgcttttcg gttcatgacg gcccgggaat ccggacgacg gttttctttc aggggtgccc
     4501 gctgcgttgc cggtggtgcc acaacccgga agcgctctcg tacgggcccg cgctcttcta
     4561 caatgatcgc tcctgcttct tttgccgggc atgcgaggcg gcgtgcaccg aggcggttca
     4621 ctccttcgtg ggggaggaac accggatgca tcgcgagcgc tgccgactct gcggtgcgtg
     4681 cgccgcggcg tgcgtcacgg gcgcgttgga gatctccggg cgcaccgtta cccccgagga
     4741 agtagtcgag gtggtcctac gcgaccgcgt cttctacgag acttccggcg ggggagtgac
     4801 gctttcgggg ggcgagccgt tggcgcagcc ggcgtttgct ggcgagatcc tcgctcgatg
     4861 ccgcgccgaa ggcctccaca ccgcggtgga tacctgcggg gagggtgctc gctaagccct
     4921 cgatgcgctg atcccgctgg ccgatctgtg gttcttcgat gtgaagcacc tcgatcccga
     4981 ggcgcaccgg gcggcgacgg ggcgctcgaa cgagcggatc ctccaaaacc tgcgctatct
     5041 cgactcggtg ctgtgccggg agggcggccc cgcgcctgcc ggcgcccggc tggtcttgcg
     5101 cgtgcccctg atcccccgca tcaacgacac cccggaaaac ctggcggcgc tggcggcgct
     5161 ggcgcgtgag cttccctcgg cgcgtgagat gcgtctgctg ccgtaccacc gttttcagga
     5221 ggacaagtac cggcgcctgg ggcgcgaata ccccggcacg gacctggtcc cgccggatga
     5281 cgcgaccgtg aaggcgatcc gcgagcgtct tgctgcagag gcgggcatca tcgtgggcat
     5341 cggcggctga attcgggcca gcaggcgccc gggtggaagc cctcccgcca tgacgccaac
     5401 aggccgcgcc gggttccgag atttggagga caccgaagcg gtcccacagg atagcccagg
     5461 gtctcctgac cctgggtacc gtgtccagaa acacacttgg ggccctgaag ggtccgacaa
     5521 gccgcgaact ggcatttgtg cgacctcttc gagttcgtgg ttatcctttg cgggagcgga
     5581 taccctgggc tgagttgtgc gaccgctttg cagccgaagc tgggctgccc gcggggctat
     5641 gggagaagag gccggagaag cccgggcatt ggagccggga tctctatgcc cctgaccgga
     5701 ctccttatct cagaagcaat agaccatgga agagtcttgc cttccgcgga gtgttctgct
     5761 atccttgtgc aaggatggcc gccaggcgga atcggcgcgg caggacaggc tgcggggcct
     5821 ccccgtgatc acctggcaat
//
LOCUS       DAOSCV010000151         5835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8707_length_5835_cov_3.803460, whole genome shotgun sequence.
ACCESSION   DAOSCV010000151 DAOSCV010000000
VERSION     DAOSCV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8707_length_5835_cov_3.803460"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1164
                     /locus_tag="PLY56_05150"
     CDS             7..1164
                     /locus_tag="PLY56_05150"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOJ20901.1"
                     /translation="MPTRRVVGLMSGTSADGIDAALCEISGSGRNARVRLLHAETVPY
                     PPEIRAEVLALSTPGDVCLSRLCRLDSILGQLFADAALKAIAAAGLQPDAIHLIASHG
                     QTLCHLPEPGGPGEWRVRATLQIAEPAVIARRTGILTVANFRAADLAAGGQGAPLVPY
                     ADWVLFTDPRRDRAVQNIGGIANVTYLPAGATLAEVRAFDTGPGNMLIDGAVRRFTAG
                     AESCDRGGARAAAGRVDPGLLAWLMSHPFLSQPPPRSTGREAYGEEFLSQVLARAASR
                     GLGEAEILATLTAYTATSIADAYRRWLPRLPEEMILGGGGVHNATLLRMIADELPTVR
                     LRTHAEFGVPDDAKEALAFALLGNEALLGVPANVPGATGGQPAILGQVAFP"
     gene            2199..3416
                     /gene="waaF"
                     /locus_tag="PLY56_05155"
     CDS             2199..3416
                     /gene="waaF"
                     /locus_tag="PLY56_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOJ20902.1"
                     /translation="MKRRQTPATFSVDRNVGFLLCQLLAPSIGKVRRAPAPAPAPEEV
                     ERIAVLKLAGLGSLVLASPTLASLRVRFPSAHITFITQLANRRLLETNIDVDEVYYLD
                     TSSPCATAAGFWRLFRHLCRGGYDLVVDLEPSSRCTALLSFLSGIPLRAGFDTPGHAR
                     GQLFNLPVPYEEHIHLVEAFHRLAERLGAPAVGRLQLTPIHYPLADEAVVESFALQQH
                     LAPERPLVAIHLGADDGPAGPRWPTERFAQLADALIAEYGVQVVFTGSAKETPLAAEA
                     LRAMHHEAVDASGHLNIPQLASLMARCALVISCDAGPLHIAAAMRTPALGLYGPTAPE
                     KEGPWGEHCTTLYHRLPCSPCSSHACGKPSRCPHGDCLRSITVPEVLGAIRARYASLL
                     RPERILAASTPAK"
     gene            3523..5370
                     /locus_tag="PLY56_05160"
     CDS             3523..5370
                     /locus_tag="PLY56_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ20903.1"
                     /translation="MRREARLRITRPGFRPRSRRFLAASVVKAYPLHPIRRAINLDGA
                     WEFAWLGEADLSSLDPARLRFDEIEAVPGVFDTGIKRYGARGTGVYRRRVPFPAPAGT
                     RLRLRLNGLGLWGRVWWDGECLGDVELPYSGYTFEITANGADCHDLAIAVDNRFDAER
                     TPLFPPYSDFYAYGGIYRSIELEELPPLRVERVRVQTLDLDAARVGLEIRLGGDVPDE
                     LTLRLAFDGDDGELVVARPEGGVVRLERVLPDARPWSPDHPELHTVTVTLAHDQIIER
                     FGLRTVAARDGAIWLNGERVRLLGINRHEAHPQLGPALPPALVAEDLQMIKALGCNFV
                     RLVHYPHDPIALDLCDELGLLVWEETMGWQLGEREFLSPRARAQMVDQARRMVSDGIN
                     HPSIILWAFLNESASDHPTAVPVYREISQAIRELDPTRLVTWASHRYERDLCWEFADV
                     VSLNFYPGWIHPTGDWSTPNLSLIAPYLAEKADYCARPGLEGKPLLVSEIGACGLYGC
                     HDRARAQWSEEFQADYFDEACRAILEEPRYAGLALWQFCDTRSYVNTGNIRVKPRGYN
                     SAGLVDEYRRPKLAYDVVARRFREARWGPFPGSGSRTPSGGAREARARR"
BASE COUNT          897 a         2069 c         1918 g          951 t
ORIGIN      
        1 gcctctttgc cgacgcgccg ggtggtcggc ctcatgtccg gcacctccgc cgatggcatc
       61 gacgcggcac tctgcgagat ctcgggatcg gggcggaacg cccgggtgcg ccttctccat
      121 gcggagacgg tgccctaccc gccggagata cgcgccgagg tgctcgccct ctccacgccg
      181 ggcgatgtct gcctgtcgcg gctctgccgg ctcgactcca tcctcggcca gctcttcgca
      241 gacgccgcct tgaaagcgat cgccgccgcc ggcctgcaac cggatgccat ccacctgatc
      301 gcctcgcacg gccagacgct ctgccatctc cccgagccag gcgggccagg cgagtggcgt
      361 gtgcgcgcga ctcttcagat tgcggagccg gcggtcatcg cgcgccggac gggaatcctc
      421 accgtcgcca acttccgggc ggcagacctg gccgcgggag gccagggcgc ccccctggtg
      481 ccgtacgccg attgggtcct cttcaccgat ccgaggcgcg accgcgcggt gcaaaacatc
      541 ggcggcatcg ccaacgtcac ctacctcccc gccggggcca cgctcgcaga ggtgcgagca
      601 ttcgacaccg gccctgggaa tatgctcatc gatggagcgg ttcgccgctt cacggccggt
      661 gccgagagtt gcgaccgagg cggcgcgcgg gcagccgccg ggcgcgtgga tcccggactc
      721 ctggcctggc tgatgagcca tcccttcctc tcgcagccgc ctccccgctc caccgggcgc
      781 gaagcctatg gcgaggagtt tctgtcccag gtgctcgcgc gtgcggcatc gcgcggtctc
      841 ggagaagcgg agatcctggc gacgctcacc gcctacaccg cgacatcgat cgccgatgcc
      901 taccggcgct ggctgccacg gcttccagag gagatgatct tgggaggggg cggcgtccac
      961 aacgcaaccc tcctgcggat gatcgccgat gagcttccca ccgtgcgctt acgcacccac
     1021 gcggagtttg gcgttccgga tgatgccaaa gaggcgctgg cattcgccct cctcgggaat
     1081 gaggccctct tgggcgtgcc ggcaaacgtg ccgggtgcaa ctggcgggca gccggccatc
     1141 ctgggccagg tcgcttttcc gtaaccagcc caaagacagt cactgtatcg gtctcgcgtg
     1201 tctgcgtggt ttggcgcact cttccgttta ggtaccgtct ggcgccgggt aaatgcttga
     1261 agatgcgcgc tctccacgta cggagtcatc tctgccgcct gggggcagca tgcctcctca
     1321 gcttgcgggg ctcgccccgc agggaaggag tcacgcgacc atgacagatg tgaagcatag
     1381 agcggccatg gatcaaggcg agaagacgga tcggcagtcg ccgcaagacc ggcggccggg
     1441 gcagccggat gcttccgggg gcgatccccc gcgcgatggt gacgagaggc cgagcaaacg
     1501 cgaccagtca cgcccagaaa cgctgccctc tgcgaccact tcgggcgggc tcccctccga
     1561 accgggctgg atgcgctcgt gagcccgccg cgggctggcg tcggcccggc cccgcgcgag
     1621 cggcgacacg gcggatgaag tggacggacg cggatgggat ggtgcgggca cctcgggccc
     1681 ccgccggcat cgacaccacg aatgagagga acgggcacgg atgggcaggg tgcaggcaca
     1741 gtgggctccc acgggcgcgg gcgatatgaa cctcgcagga tagaccagct attccgaagg
     1801 aagctttgtc tgcacccggc atcatcccaa gacgcttcca gttccatttg tccgcgttca
     1861 tctgtgtcca tctgcggatg acgatacgcc cgcgcgaatg cgccggcccc agggaaaggg
     1921 gctaccgcgt gaggctcgcg gcgctgcaga ttgacacacg gcggaacgct atgctagaat
     1981 ggtgccgggc gtgatggcat cgaagatgcc caaacgcctt gcgcggcagc tctttgcctg
     2041 ccgcaaccgc ctacgtccaa accccgaagg cgctgccgca cccgcggcca ggcagcttct
     2101 cccccaaggg gagtatcaaa gcacaggcgt gtgatgccac atttccagga aaggaggaag
     2161 gcgggggccg gattccttcc gcccgaggcg gacgcgatat gaaaagacgg cagacccctg
     2221 ctacattctc cgtggatcgc aacgtggggt tcctgctctg ccagctcctt gccccgtcca
     2281 tcggcaaggt ccggcgtgcc cccgctcctg cacccgcgcc cgaagaggtg gagcgcatcg
     2341 cggtgctgaa gcttgctggc cttggcagcc tggtgctcgc atccccaacg ctcgcgtcgc
     2401 tgcgcgtgcg cttcccaagt gcgcatatca cgtttatcac ccagctggcc aaccggcgcc
     2461 ttctggagac caacatcgac gtcgatgagg tctactatct cgatacgagt tcgccctgcg
     2521 ccacggcggc tggcttctgg cggctcttcc ggcatctctg ccgtggcggc tacgacctgg
     2581 tggtggacct ggagccctcc tcgcgctgca ccgcgctcct ctccttcctg agcggcattc
     2641 ccctgcgcgc cggcttcgac acgccgggcc atgcccgagg gcagctgttc aaccttccgg
     2701 taccctacga ggagcacatc catctcgtgg aggcgttcca ccgcctcgcg gagcggctgg
     2761 gcgcccccgc cgtcgggcgg ctccagctca cccccatcca ctacccactc gccgacgaag
     2821 ccgtggtcga aagcttcgcg ctccagcagc acctggcgcc ggagcggccg ctcgtcgcca
     2881 ttcacctggg cgcggacgac ggtccggcag ggccgcgctg gcccacggag cgcttcgcgc
     2941 aactggcgga cgcgctgatc gcggagtatg gcgtgcaggt ggtcttcacc ggctccgcga
     3001 aagagacccc cctcgcagcc gaagcgttgc gcgcgatgca tcatgaggcc gtggatgcct
     3061 ccgggcatct gaacattccc cagctggcca gcctgatggc gcgctgcgcc ctcgtcatct
     3121 cgtgcgacgc tggcccgctc catatcgcgg ccgccatgcg cacccccgcg ctgggtctct
     3181 acggcccgac cgcaccagaa aaagaggggc cgtggggaga gcactgcacc accctctatc
     3241 accgtctgcc ctgcagcccc tgtagcagcc acgcctgcgg caagccctcg cgatgccccc
     3301 acggcgactg cctccgctcc atcacggtgc ccgaggtctt gggagcgatc cgggcgcgct
     3361 acgcctcgct cctccgcccg gaacgcatcc tcgccgcatc cactcccgcg aaatgaaccc
     3421 gcccggcgcc ggccggcggg gagggacggg gcgcgtggcg aactgcttcg agagcggtac
     3481 tctgcgcggg ccggtcaggc cgcgtatcga tgaggaagga ggatgcggcg agaggcgcgg
     3541 ttgcgcatca cgcgacccgg gtttcgcccc cgaagcagaa ggtttctcgc cgcaagcgtt
     3601 gtgaaggcgt atccactcca ccccatccgg cgcgccatca acctcgatgg cgcctgggag
     3661 ttcgcatggc tgggcgaggc cgatctctcc tcgctcgacc cggcacgcct ccgcttcgat
     3721 gaaatcgagg ccgtgccggg cgtcttcgat accggcatca agcggtatgg cgcgcgagga
     3781 accggcgtct accgccggcg cgttcccttc cccgctccgg ctgggacgcg gctgcgactg
     3841 cgcctcaacg gcctggggct gtggggccgc gtctggtggg atggcgagtg cctcggcgac
     3901 gtcgagctcc cctacagcgg ctacacgttc gagatcaccg caaacggcgc cgattgccac
     3961 gacctggcca tcgccgtcga taaccgcttc gatgcggagc gcacgccgct cttcccgcca
     4021 tacagcgact tctacgccta cggcggcatc taccggagca tcgagctgga ggagctgcct
     4081 cccctgcgcg tcgagcgcgt ccgggtgcaa accctggacc tcgacgccgc ccgggtgggc
     4141 ctggagatcc gcctgggggg cgacgtgccc gatgagctca ccctccggct tgcctttgat
     4201 ggagacgatg gggagctcgt cgtggcccgg ccggagggag gcgtggtccg cctggagcgc
     4261 gtcttgcctg acgcgcgccc gtggtctccc gatcaccccg agctgcatac ggtcaccgtc
     4321 acgctcgcgc acgaccagat tatcgagcgg tttggcctgc gcaccgtcgc ggcgcgcgac
     4381 ggcgccatct ggctcaacgg cgagcgcgtg cgcctgctgg gcatcaaccg gcatgaagcc
     4441 cacccgcaac tggggccggc cctgccgccc gcgctggtcg ccgaggacct ccagatgatc
     4501 aaggcgctgg ggtgcaactt cgtccggctt gtccactacc cgcacgaccc catcgccctc
     4561 gacctgtgcg acgagctcgg cctgctggtc tgggaagaga ccatgggctg gcaactggga
     4621 gagagggagt ttcttagccc ccgcgcgcgt gcccagatgg tggaccaggc gcggcgcatg
     4681 gtgtcggatg ggatcaacca tccgtcgatc atcttgtggg cgttcctgaa cgagagcgcc
     4741 tcagaccatc ccacggcggt gccggtctac cgcgagatct ctcaggcgat ccgcgagctc
     4801 gatcccaccc ggctggtcac gtgggcctcg caccgctacg aacgcgacct ctgctgggag
     4861 ttcgccgacg tcgtctcgct caacttctac cccggctgga tccacccgac cggcgattgg
     4921 agcacgccca atctctcgct catcgccccc tatctggcgg agaaggccga ctactgcgcg
     4981 cgcccggggc tggagggaaa gccgctcctg gtgagcgaga tcggcgcgtg cgggctgtac
     5041 gggtgccatg atcgcgcccg cgctcaatgg tcagaggagt ttcaggcgga ctactttgac
     5101 gaggcatgcc gggcgattct ggaggagccg cgctacgcgg gtctggcact ctggcaattc
     5161 tgcgacacgc gctcttacgt gaacacgggg aatatccgcg tgaagccgcg tggctacaac
     5221 agcgccgggc tggtggacga ataccgccgg cccaagctgg cctacgacgt ggtcgcgcgc
     5281 cgcttccgcg aggcacggtg gggccctttc cccggatccg ggtcgcgcac cccatccggg
     5341 ggcgcccgtg aggcccgtgc gcggcgctga agcactgcgc tgctttccgg caaagcccct
     5401 tcgggccttc tcctcccagc ccttgccatt ctaaggcacc cgcgagtgca gtccaaccca
     5461 gcccggcagg gcttcgctcg caagcagcgc ggtgatttat cgccgcgcgc cgggttgcac
     5521 cctgcctccg tggcttcgga ttccttatgt cggaatctca gcgagctcga acggctggtt
     5581 gacagccagg ccgtttggag gtacactggg gagggaggat ccccctcgct tcgtgcccgg
     5641 gtggctcccc atcttccctc gccactagcc acgtggcagc ggtggatccc cacgtcactg
     5701 tgagcggcga gagcggctct ggaaggagag ataacgatga atgcacgctg gatgaaggct
     5761 ctcgcattcc tggctgccgc cggcgcaaca gccgccgtga tggcgaaatc gcgccgggat
     5821 ccctcctggc tgcgg
//
LOCUS       DAOSCV010000152         5834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8710_length_5834_cov_3.426890, whole genome shotgun sequence.
ACCESSION   DAOSCV010000152 DAOSCV010000000
VERSION     DAOSCV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8710_length_5834_cov_3.426890"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="PLY56_05165"
     CDS             complement(<1..1866)
                     /locus_tag="PLY56_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20904.1"
                     /translation="MRPLDLFSKSVWPKRHCLRSTLSARSLLLALVALVACGLPAGAQ
                     ESDEAELQRHLIQPGQLKRLVIETPLVRQGKPQAVIAIAEGEVYRQGAATINAALKET
                     VGVELPVKPAASVSLEEAGWSHCILLGHLDNNPLVAHLYHTYYDCLDAGYTGKGGYEI
                     RSVHDPFARGVNCIVAGGSDEEGAAAAAHALAKIIRDTAKGSDVVLPRLLELKLRPEG
                     RSGSIPARLSPEEAERTLAQEMALFEKPGVGRAIASNITKHAVLYHRYGDPAHLELYR
                     KLIERHIRYYAEDPYIRSSLRRYDREFRDSWAWHFVVTWDLLEEEPVFSDAERLAITN
                     HCLRLVQECVLYQRWDRPEAVQGWAQNRNIVHNHQTWPALACYFGARYFGRYYNHPYA
                     KEWKIIADGVFNGQKHAAKPQEDSASYQWLPLRHTIIWSLASGDHTFFTEGHARRAAE
                     NALMVVDNLGNQPAFGDHTDWTVTAGIPDFLGRCAWYYRDGRYLWGAMRAGQSLAGEL
                     QETYTPRVEPTPPVDHVGIVISRLPKQCYDHTERQAQYPVKPNLPYEESFDKLTFRDG
                     LDPQNQYLLLDGYARGNHMHFDANAIIHFADRGACWLVDGEYIRNGPKYHNSLTVL"
     assembly_gap    1983..1992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2102..3823)
                     /locus_tag="PLY56_05170"
     CDS             complement(2102..3823)
                     /locus_tag="PLY56_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20905.1"
                     /translation="MNRSHYWFNHHLLAVLVGLAFLVGLAAPAAAQSRAPRKKLIATG
                     WDKPDTARLRENLAQMEQRPFDGVILEAVGTRDDGKSCFLRSTFSAQAWEQRWFNKSV
                     EDLKACRFTRFTDNFVTVGANPGNVDWFDDEGWKNVVEHWRIAAWIAKEGGLKGLHFD
                     PEPYTPPHAQFNYLAQPQKAQHTFAEYQAKARERGREVMRAVIGEYPDLTIFTYFMLV
                     INSNALGHANPNAVLETSGYGLLPAFVDGWLDVIPPAVKLVDGCENAYRYNSTTQFLE
                     AAVRIKGDCQQLISPENRAKYRAQVQAGFGIYLDAYWNPPTSQWYIDGLGEPRVLRLR
                     KNVATALRVADEYVWMYGEKFRWWSTPNPRVNKETWPEALPGSEDALRFARDPADYGR
                     ARIATLEKEGKLANLARNADFASEQAASNQGVQVDWKQGGAPAGWSSWQLETSKGVFS
                     WDRETGHSGKGSARAAGVGNGCFIQSYDVKPGECYAIRAMRRIQGKGNTRIRVRWQTA
                     QGRWTLEELDKTLTTEGPANEWSEIFGVVEVPEGAGKLVVLLGMAGQSTQEDVVWFDD
                     VGVYRVD"
     gene            4122..5723
                     /locus_tag="PLY56_05175"
     CDS             4122..5723
                     /locus_tag="PLY56_05175"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ20906.1"
                     /translation="MAESPSRTRRSIAPILLLLVVTAAAFSEARSFDFVAWDDDVNLY
                     QNERFIRGTPDIAYFWRTPFFGMYIPVTYTVWGLLAAVAKQAPAAPGMPALNPSIFHL
                     ANLGLHLLNVLLVFAILRRLLDKEWAAAAGALLFGVHPIQVEAVAWATGMKDVLGGFF
                     SLLALWGYLSYAAEKRARPRGWKYAAATLAYALALLSKPSSAAMPVVAWVLDCFLLRR
                     SWRQATGALAPWAILAVPAAIATKLAQDPDASVGFITPLWARPLVAGDALAFYLGKLA
                     LPVGLIPDYSRSPASILKSGQVYYTWLVPAALAGIAWTCRRRWPWLPVSLAVFVAGLL
                     PVLGFTRFVFQELSTVADRYVYLSMLGPALALASLAVTCDLRWVRAGMGGILAVLAGL
                     TLFQSLHWYGTQPLFEHTLTVNPRSTIAHNNLGALHYMRGDYARAATHYQASLEVLPN
                     DAPVHDNLAMALLLQGKQEEAVRHWEEALRIDPGWARAHFNYGAYLASTGRMDEAREH
                     WREAVRCDPEFVPARRALEQAGDVP"
BASE COUNT          910 a         1999 c         1857 g         1058 t
ORIGIN      
        1 gagcaccgtc agcgagttgt gatacttggg gccgttccgg atgtactcgc catccaccag
       61 ccagcaggcg ccgcggtcgg cgaagtggat gatcgcgttg gcgtcaaagt gcatgtggtt
      121 gccgcgcgcg tagccatcca ggagcaggta ttgattctga gggtccaggc catcacggaa
      181 ggtgagcttg tcgaagctct cctcataggg caggttcggc ttcaccgggt attgggcttg
      241 ccgctcggtg tgatcatagc actgcttggg cagccggctg atgacaatcc ccacatggtc
      301 aaccggaggc gttggctcga cgcggggcgt gtaggtctct tgcagctcgc ccgcgaggga
      361 ttggcccgcg cgcatcgccc cccatagata gcggccatcg cggtagtacc aggcacaccg
      421 ccccaggaag tcgggaatcc ccgccgtcac ggtccaatca gtatggtcgc caaacgccgg
      481 ctggttgccg aggttgtcca ccaccatcag ggcgttttcc gcggcgcgcc gggcgtgccc
      541 ctccgtgaag aaggtgtggt cgccactcgc gagtgaccag atgatcgtat gccgcagcgg
      601 cagccactgg tagctcgcgg aatcctcctg cggcttggcc gcatgcttct ggccgttgaa
      661 gacgccatcc gcgatgatct tccactcctt cgcgtagggg tggttgtagt agcgtccgaa
      721 gtagcgtgcg ccgaagtagc aggcgagcgc cggccaggtc tggtggttgt gaacgatgtt
      781 tcggttttgc gcccacccct gcaccgcctc gggccggtcc cacctctgat acaggacaca
      841 ctcctggacc aggcggaggc agtggttggt gatcgccaga cgctccgcgt ccgagaagac
      901 gggctcctct tccaggaggt cccaggtgac gacgaagtgc cacgcccagg aatcgcggaa
      961 ttcgcggtcg tagcggcgca gtgagctacg gatgtaggga tcttccgcgt agtagcggat
     1021 atgacgctcg atcagcttcc ggtagagctc caggtgcgcc gggtcgccat agcggtggta
     1081 gagcacggcg tgtttggtaa tgttgctcgc tatcgcccgc cccacgccgg gtttctcaaa
     1141 gagcgccatc tcctgggcga gggtacgctc tgcctcttcc ggcgagagcc gcgcgggaat
     1201 cgagccggat cggccctcgg ggcgcagctt caactccagg aggcgcggga ggaccacgtc
     1261 actccccttc gccgtgtcgc ggatgatttt ggcgagcgcg tgcgcggcgg ccgccgcgcc
     1321 ctcttcgtcg ctcccgccag ccacaatgca gttcacccct cgcgcaaagg ggtcatgcac
     1381 gctgcggatc tcatagcccc ccttgccggt gtagcccgcg tccaggcaat cataatacgt
     1441 gtggtagagg tgcgcgacga gtgggttgtt atcaagatgg ccaagcagga tgcagtggct
     1501 ccacccggcc tcttccagcg agaccgacgc cgcaggcttc accgggagct cgacgccgac
     1561 cgtctccttc aacgcggcgt tgatcgtggc cgcgccctgg cggtagacct ctccctcggc
     1621 aatcgcgatc accgcctggg gcttcccctg cctgacgagc ggcgtctcga tcaccaggcg
     1681 tttgagctgc ccgggctgaa tcagatggcg ctgcagctcc gcctcgtcgc tctcctgcgc
     1741 gcctgccggg agaccgcaag cgacgagggc aacgagagcg aggaggagag agcgcgctga
     1801 tagcgtggaa cggaggcagt ggcgctttgg ccagacactt ttcgaaaaga gatcgagcgg
     1861 tcgcatacgg tctcctcctc gggcggcgcg catcgccggt aagcccgcat ccgccgctgc
     1921 cccttcgcgt ggtggatgcc gcagctgccc agtcctctta caggcagcca gtcatctcca
     1981 gcnnnnnnnn nnggcggtgc ggctgctgta cagcgcgcac gggttcgacg gccgcggcac
     2041 cggcgcgcgc ccacatatcg caggcgtacg ccggtgccag agtctacctt ctctagttcg
     2101 ctcagtccac ccggtacacg ccgacgtcgt cgaaccagac cacatcctcc tgcgtggact
     2161 ggccagccat gccgaggagg acgacgagct tcccggcgcc ctcgggcacc tcgaccacgc
     2221 cgaagatctc gctccactcg ttagccggac cttcagtggt gagcgtctta tccagctctt
     2281 cgagcgtcca gcggccctgc gccgtctgcc agcggacgcg gatccttgtg ttgccctttc
     2341 cctggatccg gcgcatcgcg cgaatggcgt agcactcgcc cggcttcacg tcgtaggact
     2401 ggatgaagca gccattcccc acgcccgcgg cacgcgccga gcctttgcca gagtggcccg
     2461 tctcgcggtc ccacgagaag acgcccttcg aggtttccag ctgccagctg ctccagcccg
     2521 cgggcgcgcc gccctgtttc caatcaacct gaacgccctg gttcgaggcg gcctgctccg
     2581 aggcaaaatc ggcgttgcgc gccaggttgg ccagcttgcc ctccttctcc agggtggcga
     2641 tacgggcgcg gccgtaatcc gccggatcgc gggcgaagcg gagcgcgtcc tcgcttcccg
     2701 gcaacgcctc gggccacgtc tccttgttca ccctggggtt cggggtggac caccagcgga
     2761 acttctcgcc gtacatccag acgtactcgt cggcaacgcg cagcgccgtc gccacgttct
     2821 tgcggagccg gagcacacgc ggttctccca ggccatcgat gtaccactgg gaggtgggtg
     2881 ggttccagta ggcatcgagg tagatcccga agcccgcctg cacctgcgcc cggtacttcg
     2941 cgcggttctc cggcgagatc agctgctggc aatcgccctt gatccgcacc gccgcctcca
     3001 ggaactgcgt ggtgctgttg tagcgatagg cgttctcgca cccgtccacc agcttcaccg
     3061 ccggcgggat cacgtcgagc cagccatcga cgaacgcggg gagaagcccg tagcccgacg
     3121 tctccagcac ggcgttcggg ttggcgtgac ccagggcgtt ggagttgatg accagcatga
     3181 agtaggtgaa gatggtaaga tcaggatact cgccgatgac ggcacgcatc acctcgcgcc
     3241 cgcgctcgcg cgctttcgcc tggtactcgg cgaaagtgtg ctgggctttc tgcggctggg
     3301 ccagatagtt aaactgcgcg tgaggcggag tgtagggctc gggatcgaag tggagcccct
     3361 tcaggccgcc ttccttcgcg atccaggcgg cgatgcgcca gtgctccacc acgttcttcc
     3421 aaccctcgtc gtcgaaccag tcgacattcc ccgggttggc gccgacggtg acgaagttgt
     3481 cggtgaagcg ggtgaaccgg cacgccttca gatcctcgac gctcttgttg aaccagcgct
     3541 gctcccacgc ctgggcgctg aaggtcgacc tcaggaaaca cgatttcccg tcgtccctgg
     3601 tgccaaccgc ctcgaggatc acgccgtcga aggggcgctg ctccatctgg gccaggttct
     3661 cacgcaggcg cgcggtatct ggcttatccc aaccggtggc aatcagtttc ttgcgcggcg
     3721 ccctgctctg cgccgccgcc ggtgccgcga gcccgacgag gaacgccagg ccaaccagca
     3781 cagcgagcag gtggtggttg aaccaataat gagatctgtt catagggtaa gcacctcccg
     3841 accgtgaagt ccccccccgc ccgaggggta gtaacagatt cgcacgagcc gctggctctc
     3901 ctgcttctaa ggaaacgcca gggaatcgcg ccgcggcccg tcacgcctcc ggcatggccc
     3961 acgcgcccgc cccgcgcgga gcggcacatc cctcgcggcg acgcgggagg cgagggcaga
     4021 gaggctcttc aacgcaggag tcggcgagcg ccacccggaa tcatacagta tgtttgtgct
     4081 tgcccggagt accccaacaa cgcgctggct cggaggcgga tatggctgaa tcgccgtctc
     4141 gcacccggcg atccattgct cccatcctgc tcctcctggt ggtcacggct gccgccttct
     4201 cggaggcgcg ctcgttcgac ttcgtcgcct gggacgacga tgtcaacctc taccagaacg
     4261 aacgttttat aaggggcacg ccagatatcg cctacttctg gcgcacgccg ttctttggga
     4321 tgtacattcc ggtcacctac acggtgtggg ggctgctcgc cgccgtggcg aagcaggcgc
     4381 ccgccgcgcc cggaatgccg gcgctgaacc cgtctatctt ccacctggcc aacctggggc
     4441 tgcacctgct caatgtgctc ctggtcttcg ccatcctgcg gcggcttctg gataaggagt
     4501 gggccgcagc ggccggcgcg ctcctcttcg gggtacatcc gatccaggtg gaggccgttg
     4561 cctgggccac cgggatgaag gacgtgctgg gcgggttctt ctcgctcctg gccctgtggg
     4621 gctatctctc ctacgcggca gagaagcgcg ctcgcccccg aggctggaag tacgcagcgg
     4681 ctaccctcgc ctacgccctc gccctcctct cgaagccaag ctccgcggcg atgccggtgg
     4741 tggcgtgggt cctcgattgc ttcctgctgc gccgctcgtg gcgccaggcc acgggggcgc
     4801 tggcgccgtg ggccatcctc gccgttccgg ccgcgatcgc gaccaagctt gcccaggatc
     4861 ccgatgccag cgtcggcttc atcacgccgc tctgggcccg gcctctcgtc gctggcgatg
     4921 ctctcgcgtt ctacctcggg aaactggcgc ttcccgtggg cctcatcccc gactacagcc
     4981 gctcgcccgc ctccattctg aagagcggtc aggtctacta cacctggctc gtgccggccg
     5041 cgctcgctgg catcgcctgg acctgccgga ggcgctggcc gtggctgccg gtctcgctcg
     5101 cggtcttcgt cgccgggctg ctgcccgtgc ttggcttcac gcgcttcgtc tttcaggagc
     5161 tctccaccgt cgccgaccgt tacgtctacc tatcgatgct cgggccggca ctcgccctgg
     5221 ccagcctggc agtcacctgc gatctccgct gggtgagggc cggcatgggc ggcattctgg
     5281 cggtgctggc ggggctgacg ctcttccaga gcctgcactg gtacggcacg cagccgctct
     5341 ttgagcacac gttgacggta aacccacgca gcaccatcgc gcacaacaac ctcggggcgc
     5401 tccactacat gcggggagat tacgcccgag ccgccacgca ctatcaggca tcgctcgaag
     5461 tgctcccgaa cgatgctccg gtgcacgaca acctggccat ggcgctcttg ctccagggga
     5521 agcaagaaga ggcagtgcgt cactgggaag aggcgctgcg catcgacccg gggtgggcgc
     5581 gcgcgcactt caactacggt gcctatctgg ccagtacggg caggatggac gaggcgcgcg
     5641 aacactggcg cgaagcagtg cgctgcgatc ccgaattcgt gcccgcccgg cgcgctctgg
     5701 agcaggccgg agacgtgccg tagagcgtgg tctcttcccc cgcgttttcc gcccctccgc
     5761 catctccccc aacacgccat ccctccacct cgccccactg gcgtacaact tgcacactcg
     5821 ggcacccagg aaag
//
LOCUS       DAOSCV010000153         5807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8763_length_5807_cov_3.335188, whole genome shotgun sequence.
ACCESSION   DAOSCV010000153 DAOSCV010000000
VERSION     DAOSCV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8763_length_5807_cov_3.335188"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            239..514
                     /locus_tag="PLY56_05180"
     CDS             239..514
                     /locus_tag="PLY56_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOJ20907.1"
                     /translation="METITLPESKLPGTGQGDYCVIIFNNNTNSFDEVIHILQVATGC
                     PYEEAEMETWEAHHRGQAYVHFADREECDRVASIISSIGVRTEVCEF"
     gene            complement(673..1734)
                     /locus_tag="PLY56_05185"
     CDS             complement(673..1734)
                     /locus_tag="PLY56_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ20908.1"
                     /translation="MEREYYLTLARSGLRMPIGADLVVREQEDPDAVLTDGVRLGRVL
                     EETARRFRTPLAVPHMDLELEKKDLLRAIGVPEAAASTFHFASCPDAKLFAKAEDGLR
                     GAPSVRLQAAADAIRYIARHTDLLPIGMCIGPFSLMTKLLADPITPVFLAGMGTTAED
                     DDEVRRMEGVLELGMRAIEHSITVQVEAGARMIFIAEPAANTVYLSPKQMAAGSDAFE
                     RYVMRYNRRVKELLDRHGVDLFFHCCGELTDAMVAQFASLDPAILSLGSSRRLWEDAA
                     LVPKNVVLFGNLPSKQFYSDALITAEDVVARAAELTARMHQAGHPFILGTECDVLSVP
                     DCHATILRKVMALIEGAAT"
     gene            complement(1971..3137)
                     /locus_tag="PLY56_05190"
     CDS             complement(1971..3137)
                     /locus_tag="PLY56_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ20909.1"
                     /translation="MHAPNPISSAWLVTEFFEVARDYRRRDPERVLMLVDAHGTPLAP
                     DEPFPCASCGCGISDWHQMVVESLRWGEPYLDTCPEGHYLWAVPLTFNSTLVGGVVAT
                     FARGARGATIGSAVGAAARELLEIVTSRNLTNAALLRENRQAAECEASKACAIHGLKE
                     RMYTSVREMYLREEEPLRAAIGARDRRAAREVLNRILVGVYAVGTQDLELLKAVVLEF
                     LVVIYRAAVEAGGDPVQMLGLNYQSYLSLFRISDEEHLTHWLVRHLERLMDCLELEPK
                     RPPEARLQEAVRYMQEHFPEDLGREQVAAVVGLSPSHFSHLLRDRMDTSFRDLLNEIR
                     IRRACDFLRRTERSLAQIAQDVGFADQSYFTKVFHRHLGCTPREYRISHARRGL"
     gene            3699..4994
                     /locus_tag="PLY56_05195"
     CDS             3699..4994
                     /locus_tag="PLY56_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ20910.1"
                     /translation="MGVNHRRNRRLILGFELIWGTGLPFLQSTTTLPGYLASLGVSGF
                     LIGLVPALFAGGIAIVQPFSLYLIRPGPRRLHHFLWAYRLGIFCYLAMAASAAFLPAD
                     AVTARVALFFICYAGYVFVAGAGDPHYIAVIVESVPKGERGWFFGQRQICFGLGGIIG
                     GVFAVHALRALPAPANFGLSIFIGALLLMLATIHFSRFEYVAQGDPGKRPPLLSCLVD
                     VYHLARRQRSFLLFCAVTALFVLAQGPFAFLALYVKDVLHAGDGLYGKLGFLTMASSL
                     VFAFLLGRAGDRAGHRRAFMMGVSLYAVGLVLMLVGASSTLLLGAYFLAAICGPTWAV
                     CGFNLGYECSDEPDPARVYAGVSLVSAPLRIIGPPAAGAAIDQWGHAAIIAGAAGLAL
                     MALLILALLPARKAVREHPGDASREPGGASQPARAGRRA"
     gene            5215..>5807
                     /gene="rpsB"
                     /locus_tag="PLY56_05200"
     CDS             5215..>5807
                     /gene="rpsB"
                     /locus_tag="PLY56_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ20911.1"
                     /translation="MKDLLEAGVHFGHQTRRWNPKMKRYIYGGRNGIYIIDLHQTLKL
                     FEEAYEFIRETAAEGEVVLFIGTKKQAQDAVREAAEQCRMFHVSQRWLGGMLTNWSTI
                     QKRIQRLRELERMEETGDIARRPKKEQAVLYEEKEKLERILGGIKEMPKLPGAVFVVD
                     LRKEHIAVKEARKLGIPVVAIVDTNCDPDEVDYVIPG"
BASE COUNT         1006 a         1828 c         1905 g         1068 t
ORIGIN      
        1 ccggggtcct tcaagggctg tcgcatctac tcgcggcagc ccttttgctt gtaccgctcc
       61 ataaaggatc gctcatctca ggggacgtaa ccgaggaatc agggatgaga gagtgacaca
      121 ggccttcgcg tgagctgccc ggtacgactc gcaggcgctg gcttcggtga ggggccgtct
      181 tgggaaagag gtggttggag cctaccggga gagctcgctt cgagataggt ggtgagtgat
      241 ggagacgata acgcttccag aaagcaaact gcctggaacc gggcagggcg actattgcgt
      301 gatcatcttc aacaacaaca cgaactcgtt tgatgaggtg atccacatcc ttcaggtggc
      361 caccggctgc ccctatgagg aggcggagat ggagacgtgg gaagcccacc atcgcggtca
      421 agcctacgtc cactttgccg accgcgagga gtgtgaccgg gtcgcgagta tcatctcttc
      481 catcggggtg cgcacggaag tgtgcgagtt ctgaagggcc ggccaagttc tgaagggcgt
      541 ggctgttgcg tcacagacgg catgcaggcg cccctgcgtt gcccgccctt ggggcacccg
      601 cggatcgggg ttcgccacgg gtgagattcc gcttccggcc ggcgccgcca caccggccgg
      661 aagcaccgcc agctatgtgg cggcaccttc gatgagggcc atcacctttc gcagaatagt
      721 cgcatggcaa tcgggcacgc tcagcacgtc gcactcagtt cccaggatga aggggtgtcc
      781 tgcttgatgc atgcgggcgg tcagctctgc tgctcgcgcg accacatcct ctgccgtgat
      841 cagcgcatca gagtaaaact gcttcgaggg caggttcccg aacaggacga cgttcttggg
      901 caccagggcg gcatcctccc acagccggcg cgagctgccc agactgagaa tggcaggatc
      961 gagcgaggcg aactgtgcca ccatcgcatc cgtgagctca ccgcagcaat ggaagaaaag
     1021 atcgacgcca tgccggtcga ggagctcctt tacacggcga ttgtagcgca tcacgtaccg
     1081 ctcgaaggca tccgaacccg cggccatctg cttgggagag agatagaccg tgttcgctgc
     1141 cggctccgcg atgaagatca tccgtgcgcc tgcctcgact tgcacggtga tagagtgctc
     1201 aatggcgcgc atccccagtt ccagcacgcc ctccatccgg cggacctcgt catcatcctc
     1261 cgcggttgtc cccatgccgg ccagaaagac gggcgtgatc gggtctgcga gtagctttgt
     1321 catcagcgag aaggggccga tgcacatccc gatcggtagc agatccgtgt gccgggcaat
     1381 atagcggatc gcgtcggccg cggcttgtag ccggacgctc ggcgctcctc tcaggccatc
     1441 ctccgccttt gcaaacagct tcgcgtccgg gcaggaggca aagtggaagg tgctggcggc
     1501 tgcctctgga actccaatcg cgcgcagcag atctttcttc tccagttcca ggtccatatg
     1561 cggcacggcc agtggtgtgc ggaagcgacg agccgtctcc tccagtacgc ggcccaaacg
     1621 caccccatcc gtgagcaccg cgtccggatc ctcctgctcc cgaactacta ggtccgcgcc
     1681 gatgggcatc ctcaggccgg agcgcgccag cgtcaggtaa tactctctct ccatcgactc
     1741 tctccctttt ctcgtcgcga gaacagttgc ctcggggtcc ggacgccagc aagagacgtc
     1801 ctctgaccat gatcatacgg caaagaggcg ctcacgttct gtaacaacgg tgcgtttgtg
     1861 gtggtaactt cgctcgctgt tttggcattt ttcgaggaaa gttggtcacg gcgtgaggcg
     1921 gcagggggga tgccatcgaa ggcgatgccg gggaattcgc taccggagga tcaaaggccg
     1981 cggcgtgcgt gagagatgcg atactctctc ggagtgcacc cgaggtggcg gtggaagacc
     2041 ttggtgaagt agctctgatc ggcgaaaccg acatcctggg cgatctgcgc caggctgcgc
     2101 tcggtgcgcc ggaggaagtc gcacgcccgg cggatgcgga tctcgttcag gagatcccga
     2161 aagctggtgt ccatgcgatc gcgcaggagg tgagagaagt gtgaggggct cagcccgacg
     2221 acggcggcca cctgctctcg gcccagatcc tccgggaagt gctcctgcat atagcgcacg
     2281 gcctcctgga gacgcgcctc cggggggcgc ttcggctcga gctccaggca gtccatgagg
     2341 cgctccaggt ggcgaaccag ccagtgagtc aggtgctcct catcggagat gcggaagagg
     2401 ctgagatagg actgatagtt cagtccgagc atctgcaccg ggtcgcctcc agcctcgacg
     2461 gcggcccggt agatgacgac gaggaactcc aggaccaccg ccttcaggag ctccaggtct
     2521 tgggtgccga ccgcatagac gccgacgagg atgcggttga gcacctcgcg ggcggcgcgc
     2581 cggtcccggg cgccaatggc agcgcggagc ggctcctctt cgcggaggta catctcgcgg
     2641 accgaagtgt acatccgctc cttcagaccg tggatcgcgc acgccttact tgcctcgcac
     2701 tccgcagcct ggcgattctc acgcagaagc gcggcattcg tcaggttgcg tgacgtcacg
     2761 atctccagga gctcccgggc tgccgcgccc acggcgctcc cgatagtcgc cccacgggcg
     2821 ccgcgcgcga aggtggcgac cacgccaccg acgagcgtgc tgttgaaggt gagcggcacg
     2881 gcccacaggt agtgaccctc ggggcaggta tcgaggtagg gctcgcccca acggagcgac
     2941 tccacgacca tctgatgcca gtcggagatc ccacaaccgc aggatgcgca agggaacggc
     3001 tcatccgggg caagcggcgt gccatgcgcg tccacgagca tgagcactcg ttccggatcg
     3061 cggcgccggt agtcgcgcgc cacctcgaaa aactccgtca cgagccaggc gctggatatc
     3121 gggttcggcg cgtgcacctc tcggtctact gccttcctgc gtcggaatgg gggctcgcgc
     3181 catttcgatg acggtcgagc cggctggaga cgccggagac ctccctatcc aaaggcgtcg
     3241 aactcttccc caccatcaat tgtttcccat ccgggcaacc cgattcctgc cgcaggcgcg
     3301 tgacacgtcg gcagggaacc gtggcgcgct cgctagggct tcggtcggcc gtgcccttgc
     3361 aggcgcgtgt gcccgaccat actaagggcc ccgggccaaa cgcccctttg gggtcgcttg
     3421 cgaaccttcc aagcgagagc cgggtgccac gggctacccg catccaaacg gcgagctatc
     3481 cggggcgcgc gcgaagggaa accggtcgca acggctaatc tgagagggga gccgccgtga
     3541 aagatccccc ggcgtgatat ggaggcgttg cccatgagca cgtttcgtga ggtgcaattc
     3601 gtgagactcg atggtcaccg tccactaccc cacgcggcgg agtggggcag ttccgatctg
     3661 ttgggatggc gggggatctc ccgaggggcg cgacagcgat gggagtgaac caccgtcgca
     3721 atcggcgcct cattctgggc ttcgagctga tctggggaac ggggctgccc ttcctgcagt
     3781 caaccaccac tctccccggc tacctggcct cgctgggcgt ctccgggttc ctgatcggcc
     3841 tggtcccagc cctgtttgcc ggcgggattg ccatcgtcca gcccttttcc ctctatctga
     3901 tccggcccgg tccgcgtcgg cttcaccact tcctctgggc ctaccggctc ggcatcttct
     3961 gctacctggc gatggcggca tcggcagcct tcctcccggc ggacgccgtc acggcccggg
     4021 ttgcgctctt cttcatctgc tacgccgggt acgtctttgt ggccggagcg ggcgacccgc
     4081 actacatcgc ggtgatcgtg gagagcgtgc cgaaggggga gcggggttgg tttttcgggc
     4141 agcgccagat ctgttttggc ctgggtggca tcatcggcgg cgtgttcgcg gtgcatgcgc
     4201 ttcgcgcgtt gccggcgccg gccaacttcg gcctgagcat cttcatcggc gcgctgctcc
     4261 tcatgctggc cacgatccac ttttcccggt tcgaatacgt cgcgcaggga gacccaggca
     4321 agcgcccgcc ccttctctcg tgccttgtcg atgtctatca tctggcgcgc cgccagcgga
     4381 gcttcctgct cttctgcgcg gtgaccgcgc tcttcgtgct agcccagggg ccgttcgcgt
     4441 ttctcgcgct ctacgtcaag gatgtcctcc atgccggtga tggcctctat ggcaagctgg
     4501 gcttcctcac tatggcgagc agcctggtct tcgcctttct cctggggcgc gctggcgacc
     4561 gcgcgggaca tcggcgggcg ttcatgatgg gcgtctctct ctacgcggtg gggctggtcc
     4621 tgatgctggt tggggcgtcg tccacgctgc tcctaggcgc ctacttcctg gccgccatct
     4681 gcgggccgac ctgggccgtc tgcgggttca acctgggcta cgaatgctct gatgaaccgg
     4741 atccggcacg cgtctatgcc ggagtgagtc tggtctcggc gccgctgcgg atcattggcc
     4801 ctcccgccgc cggcgccgcg atcgatcagt ggggccatgc cgcgattatc gcgggtgcgg
     4861 ccggcctcgc tctcatggcg ctcctgattc tggccttgtt gccggcgcga aaagcggtcc
     4921 gggagcaccc gggggatgcg tcgcgcgagc cgggtggcgc cagccagcct gccagggccg
     4981 ggcggcgtgc ctgagcgggc gagcttcgcg cgccaattct tgcattttgg tatcgcatat
     5041 ggtaaactac accggaatga cacacctttc ctgacccgag acagggtggc aggcggccga
     5101 tagctggtgg actacgccgc gcgggcgcgg atgctgtgcc tcgggaacga cgggaaggga
     5161 ggctgaacca gaaaggagac ccagagtttg tctacaacac agccgcacgt cagcatgaag
     5221 gacctgttgg aggccggcgt ccacttcggc catcaaaccc gccgttggaa cccgaagatg
     5281 aagcgctaca tctacggcgg gcgcaacggc atctacatca tcgatttgca ccagaccctg
     5341 aaactctttg aggaagccta cgagttcatc cgcgagaccg cggccgaagg cgaagtggtg
     5401 ctcttcattg gtacgaagaa acaggcccag gatgctgtgc gagaggccgc cgagcagtgc
     5461 cgcatgttcc acgtcagcca gcgctggctt ggtgggatgc tgacgaactg gtccaccatt
     5521 cagaagcgga tccagcgcct ccgtgagctg gagcgcatgg aagagaccgg cgatatcgcg
     5581 cggcgcccga aaaaggagca ggctgtcctt tacgaggaga aggagaagct ggagcggatc
     5641 cttggcggga tcaaggagat gccgaagctt cccggagcgg tcttcgtggt cgatctgcgc
     5701 aaagagcaca tcgccgtgaa ggaagcgcgc aagctgggca tcccggtggt cgccatcgtg
     5761 gatacgaact gcgatccgga tgaagtggac tacgtgatcc cgggcaa
//
LOCUS       DAOSCV010000154         5804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8770_length_5804_cov_3.296051, whole genome shotgun sequence.
ACCESSION   DAOSCV010000154 DAOSCV010000000
VERSION     DAOSCV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8770_length_5804_cov_3.296051"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3316)
                     /locus_tag="PLY56_05205"
     CDS             complement(<1..3316)
                     /locus_tag="PLY56_05205"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ20912.1"
                     /translation="MVRTAVLALVLLLGGPALLAAPIDGRSAGRWVAQPGWSPGRTAE
                     VYGLSGQGEWLEFWAEGKDRLMTWTLRPIPEELGHEPRYLLFTYRAFNLDNTVENYVL
                     SVRDGSPSWRQYVSATDLVTDGQAHTVAIDLFSYRPPEPINWFALRIAPASDARGRLL
                     ARITFADTLPPGVTARTAPPIEPRTQRIEVEKVSWNPSPHFTPRPPTQHEMTPTPAGI
                     RFRMSGYNRSMRWSASAPEGLDLGAMPYVSIRYRARGRFGPFGYALHISAAGTQGEKL
                     GESVMEPGDVEDEGAWHVFRRQLPFAGTAQSLIIGIDSLSPDAEIEMDYIEFSSVPPA
                     DPISQVVEYERRPGPWPRGKDGLTTVALPRAAVPPNIYMIPRMGIGAWFDTMHITVDG
                     IPFEVPDKVSAMPATGTAAEDSVTVDLPRGTTEALILLAAAFPRGEMFGTNWSRPTPL
                     RLLSEPGRVVLEFIYADGTSDQMLPVHAAKKTYGIGRGIAVYAARPSPGKTPVRLALH
                     DRMRNACFGILGVTANTGKPRVPEPARAQVWYPPVKKPPLSDATFSFRTEGGLTWRAI
                     ESPMLGGSVSLAESPVFLMKLGEKEIPSSRWRVQSVQENAGETRITSTYEEAGVALRA
                     VLVARREERNSVRLSLELVNEGTNPVTGTLFFPYVSGLKIGSVDETWYFVARRGGVIH
                     HVPCAWRDEIGEKHPLQVDGFFNPRIGAGICFLPRDTEERFRWYRVEKDESGGTYALE
                     FLPETVQPKRGWSSVPVVVAVVPGDWKDQFNTYLKWVKTWHKPTVPRKQWFREVFAFC
                     PGDPTSNMSLPVEQRIDFVSKARRFKDVIGVCDYVHLFGWAKTEEYGHWGDYDHYDAI
                     GGRERFVQEVRRIQETGTPLGLYLDGYLVSTRSLKPTQEEREKWAVRQADGKMLYHTS
                     YVAHSMCPYVPEWRDYLTKVYQRVAAEVKPDGMYLDEFGKSMVSRICYSAEHGHPVPM
                     GMAPGEGILSRQVREAIPPEIATYCEFVPSDVMTQYLDGGFGHVALDGHRDAGDPVAP
                     HYVNLHRFAFPDFKTFELIYYVPQTNGNWYLLKYPFFNGDGYYLTSVLFSSDEHAQGF
                     YRSVFRVQHEHKDAFTA"
     gene            complement(3580..4104)
                     /gene="tpx"
                     /locus_tag="PLY56_05210"
     CDS             complement(3580..4104)
                     /gene="tpx"
                     /locus_tag="PLY56_05210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOJ20913.1"
                     /translation="MVQKRTGIITFNRKPMTLVGPELQPGDAAPDFRVLKANLAPVTL
                     ADSAGKVRLISVIPSIDTPICHAQTRRFNEEAATLPENVAILTISADLPFALRRWRAA
                     EGIDRIQLLSDYQEHSFGRAYGVLIDELKLLARAIFVVGADGKIVYREIVPEVNQHPD
                     YDAALKAARQAAGE"
     gene            complement(4218..5612)
                     /locus_tag="PLY56_05215"
     CDS             complement(4218..5612)
                     /locus_tag="PLY56_05215"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ20914.1"
                     /translation="MIRMARVGRLCVTGFLAVLAWWSAMPASWAQNRPHLTRLRNRLT
                     VITKEVHSSNVVAVSVFVRGGVSAEPADLPGLANFTHHMLLRGTTHRTAEEIMAPIEA
                     IGGTLRARADHDYSMIYLLCAADALRTGLTVMSDVIRNPRFDPAEVEKQREQILSAFT
                     RLDQDPRWVLQKEMGRLLYASGPYSRVVPGTPESVKRITREQLVSFHRQQYTPENLIV
                     VVVGNIDRAAAEESVARAFGDMSFSMRPPAVPQPSTWRPLGDLGTNVAVRSQATDLAH
                     LLIGFPMGAITREEYPAVLVLNSLLGGGMGSRLMREVRERQGLSYSPGTYLAEYCGPS
                     YLAAYIPTEPVRLVFGLNGPYHTPEMMLNRVKDALLEQFDWVRQHPVSEAELQRARRY
                     TIGTFIYDHQRTVNQARYLGWFELSGLGYQYDEELPKAIEKVTKEDILALARKYFTHY
                     AIALIVPESNSERR"
BASE COUNT          957 a         1908 c         1947 g          992 t
ORIGIN      
        1 ctgccgtgaa cgcgtcctta tgctcgtgct gcacccggaa gacggagcgg tagaaccctt
       61 gggcgtgctc gtcgctgctg aacaagacgc tcgtgaggta gtagccgtcg ccgttgaaga
      121 acgggtactt cagcaggtac cagttgccgt tcgtctgcgg cacgtagtag atcagctcga
      181 acgtcttgaa gtccgggaac gcgaaccggt gcaggttcac gtagtgcggc gccaccggat
      241 ccccggcatc gcgatggccg tcgagcgcga cgtgcccaaa gccgccgtcc agatactgcg
      301 tcatgacgtc ggaggggacg aactcgcagt aggtggcgat ctcaggcggg atcgcctcgc
      361 ggacctgccg cgacaagatt ccctctccgg gcgccatccc catcggcacg gggtgaccat
      421 gctccgcgga gtagcagatc cggctcacca tcgacttccc gaactcatcc aggtacatcc
      481 cgtccggctt cacctcggcc gccacgcgct ggtagacctt cgtgaggtag tcgcgccatt
      541 ccggcacgta cgggcacatc gaatgggcaa cgtagctcgt gtgatagagc atcttgccat
      601 ccgcttggcg gacggcccac ttctcccgct cctcttgcgt gggcttcagc gagcgcgttg
      661 aaaccaggta gccgtcgagg tagagcccga gcggggttcc cgtctcctgg atgcggcgca
      721 cctcctggac gaaccgctcg cgcccgccga tggcgtcgta gtggtcataa tcgccccagt
      781 ggccgtactc ctccgtcttg gcccagccga agagatggac gtagtcgcag acgccaatca
      841 cgtccttgaa ccggcgtgcc ttgctcacga agtcgattcg ctgctccacc ggcagactca
      901 tgttggacgt gggatcgccc gggcaaaacg cgaacacctc gcggaaccac tgcttgcgcg
      961 ggacggtcgg tttatgccag gttttcaccc acttcaggta ggtgttgaac tggtccttcc
     1021 agtcgccagg aacgacggca acgaccaccg gcaccgagct ccagcctcgc tttggctgga
     1081 cggtttccgg caggaattcg agcgcgtagg tgccgccgga ctcgtccttc tccacgcggt
     1141 accagcggaa ccgctcctcg gtgtcgcgcg gcaggaagca gatccccgcg ccgatccggg
     1201 ggttgaagaa gccatccacc tgcagcgggt gcttctcgcc aatctcgtcg cgccaggcgc
     1261 acggcacgtg atggatgacg cccccacggc gggcgacgaa ataccacgtc tcatccaccg
     1321 acccgatctt gaggccagag acgtagggga agaagagcgt tccggtgacc gggttcgtgc
     1381 cttcgttcac cagctccagc gagagccgca cgctgttgcg ctcctcgcgc cgggccacca
     1441 ggacggcgcg caacgccact cctgcctcct cgtaggtgct cgtgatgcgc gtctcgccgg
     1501 cattctcctg cacgctctgg acgcgccagc gcgacgaggg gatctccttc tcaccgagtt
     1561 tcatcaggaa gaccgggctc tccgcgagag aaacgctccc gcccagcatg ggagattcga
     1621 tagcgcgcca cgtgagaccg ccttccgtgc ggaaggagaa ggtcgcgtcc gaaagcggcg
     1681 gcttctttac gggcggatac cacacctgcg cgcgcgccgg ctctggcacg cgcggcttcc
     1741 cggtgtttgc cgtcaccccc aggatcccga agcaggcgtt tcgcatccgg tcgtgcagcg
     1801 ccagccggac cggcgtcttg cccggcgagg gccgggccgc atagaccgcg atcccgcgcc
     1861 cgataccgta ggtcttcttc gcggcatgca ccgggagcat ctgatccgag gtcccatcgg
     1921 cgtagatgaa ttcgaggacc acgcgtcctg gctccgagag caggcgcagc ggggtgggcc
     1981 gagaccagtt ggttccgaac atctcgccgc gcgggaaggc ggctgccagg aggatcagcg
     2041 cctccgtggt cccgcggggc aggtcgacag tcacagaatc ctcggcggcc gtgcccgtgg
     2101 ccggcatcgc ggagaccttg tctggcacct cgaacgggat tccatccacg gtgatgtgca
     2161 tcgtgtcgaa ccacgcgccg attcccatgc gcgggatcat gtagatgttg ggcggcacgg
     2221 cggcgcgggg gagcgccacc gtcgtgagcc cgtccttacc ccgcggccag ggcccgggcc
     2281 gacgctcgta ctccaccacc tggctgatcg ggtctgccgg aggcacagac gagaactcga
     2341 tgtagtccat ctcgatctcg gcgtccggcg agagggaatc gatcccgatg atgagcgatt
     2401 gcgcggtgcc ggcaaacggg agctgccgcc ggaagacgtg ccaggcgcct tcatcctcca
     2461 catcgccggg ctccatgacg gattcgccga gcttctcgcc ttgtgtgccg gcagcgctga
     2521 tatggagcgc gtagccaaac ggcccgaatc gcccgcgcgc gcggtagcga atcgagacat
     2581 acggcatcgc ccccaggtcg aggccctccg gagcgctcgc ggaccaccgc atcgagcggt
     2641 tataccccga catacggaag cggatgcccg cgggcgtcgg ggtcatctca tgctgcgtcg
     2701 gggggcgcgg ggtgaaatgg ggcgagggat tccaggagac cttttccact tcaatccgct
     2761 gggtgcgtgg ctcaattggc ggggcggtgc gcgccgtcac ccccggcggc agcgtgtccg
     2821 cgaaggtgat ccgggcgagc agccggcccc gggcgtccga cgcgggggcg atccggagcg
     2881 cgaaccagtt gatcggctcc ggcgggcggt agctgaacag gtcgatggcg accgtgtgag
     2941 cctggccgtc tgtcaccagg tcggtggcgc tcacgtactg ccgccacgag ggcgagccat
     3001 cccggacgga aaggacgtag ttctcaacgg tgttgtcgag gttgaacgcg cggtaggtga
     3061 agaggaggta gcgcggctcg tggcccagct cctccgggat gggccgcagc gtccaggtca
     3121 tcagccggtc cttgccctct gcccaaaact ccaaccactc tccctggccg ctcaacccgt
     3181 agacttccgc cgtgcgcccc ggagaccatc cgggctgcgc tacccagcgc ccggcggacc
     3241 ggccatcgat cggcgccgcc aagagcgcgg gccccccgag gagcaagacc aacgcaagca
     3301 ctgccgttcg taccatgata cttccccaac ccatctgcag gggtcttcgc gatgtgaagc
     3361 cctccgactg gcccccgact cgcccggatc gtgactatcc ggcgatacag gtcactctga
     3421 tgaggcagga caaaggtacc tgcccgctcg caatggcgcg tcccgccatg cgggggcggc
     3481 ggccttcgcc ctgcccacac ccggctgctt cactcagatc gatccgtcgg tgcctcccgg
     3541 ctacccaggt ctgcacatcg gctcggagcg tgaggcggcc tattcgccgg cggcttgccg
     3601 ggccgctttg agcgccgcgt cgtaatccgg gtgctggttc acctcgggca cgatctcgcg
     3661 atagacgatc ttgccatcgg cgcccaccac aaagatggca cgcgccagca gtttcagttc
     3721 gtcgatgagg acgccgtagg cccggccgaa ggagtgctcc tggtaatccg agaggagctg
     3781 gatccggtcg atcccctcgg cggcccgcca gcggcgaagg gcaaagggga ggtcggcgct
     3841 gatggtgagg atggcgacgt tttcggggag cgtcgcggcc tcctcgttga atcggcgcgt
     3901 ctgcgcatgg cagatgggcg tgtcgatcga ggggatgacg ctgatcaggc gtactttgcc
     3961 ggctgagtct gcgagggtga cgggcgccag gttggccttc agcacgcgga aatcgggtgc
     4021 agcatcaccg ggctggagtt ccggtccgac cagggtcatc ggctttcggt tgaaggtaat
     4081 gataccagtt cgcttctgaa ccatgggttt ctcctggagc agccgccatc aagggggacg
     4141 aagcgcgtgg ctacgaccac tcaccgattt cacggggtcc accgcggagt taggcatcgc
     4201 ggtgaacaga gccttttcta ccggcgttcg ctattcgatt cgggaacgat cagggcgatg
     4261 gcgtagtggg tgaagtactt gcgcgccagc gccaagatat cctctttcgt tactttctcg
     4321 attgccttcg gcagttcctc atcatactgg tagccgaggc cggatagctc gaaccagccc
     4381 aggtaacgcg cctggttcac cgtgcgttga tggtcgtaga tgaaggtgcc aatggtgtag
     4441 cgccgggcgc gctgcagctc tgcctccgag acggggtgct gccggaccca gtcgaactgc
     4501 tcgaggaggg catccttcac ccggttcagc atcatctccg gggtgtggta gggaccgttc
     4561 agcccgaaga cgaggcgcac gggctccgtc gggatgtagg cggccaggta gctcggcccg
     4621 cagtactcgg cgaggtacgt gcccgggctg tagctcaggc cctggcgctc gcgcacctcg
     4681 cgcatcagcc gcgagcccat gccgccaccg agcagcgagt tgagcaccag aacggcggga
     4741 tactcctcgc gagtgatggc gcccatcggg aaaccgatca gcaggtgcgc gaggtcggtc
     4801 gcctgactcc tgaccgccac gttggtgcca aggtcaccca ggggacgcca ggttgagggc
     4861 tggggtacgg ctggcgggcg catcgagaag gacatgtcgc cgaaggcacg tgcgacgctc
     4921 tcctcggcgg cggcccggtc gatattgccg acaaccacca cgatcagatt ctccggggtg
     4981 tattgctgcc ggtggaagga gaccagctgc tcgcgagtga tccgcttcac cgattccggc
     5041 gtgcccggca caacgcgcga gtagggcccg gaggcgtaga gaaggcggcc catctccttc
     5101 tggagcaccc accgcggatc ctgatccagg cgagtgaaag ccgagagaat ctgctcgcgt
     5161 tgcttctcca cttccgccgg gtcaaaccgg gggttgcgga tcacgtctga catgacggtg
     5221 agcccggtac ggagggcatc ggcggcgcag aggaggtaaa tcatggagta gtcgtgatcc
     5281 gcgcgcgcgc ggagcgtgcc gccgatcgcc tcgatgggcg ccatgatctc ctcggccgtg
     5341 cggtgcgtgg tgccgcgcag gagcatatgg tgggtgaagt tggccaggcc ggggaggtcg
     5401 gccggctcgg cggagacgcc gccgcgaacg aagacgctca cggccaccac gttgctgctg
     5461 tgcacttcct tcgtgatgac cgtgagacgg ttgcgcaacc gggtcaggtg cggccgattc
     5521 tgcgcccacg acgcgggcat ggcggaccac cacgccagga ctgccaggaa tcccgtcacg
     5581 cacagccggc cgaccctcgc catccgtatc attcggctcc tcctgcctcg ctcggggtgg
     5641 caccggacgc tgcctggttc cccgactgca tatcgccacc ttcaggcggc tgaggccctt
     5701 cccccgcggg ttgcgcgccg gccggggcag acgccccttc tggccgggta ccctccgtgg
     5761 tgggggtggg tgcctcctct gggcgggtac tctccgcggc cggg
//
LOCUS       DAOSCV010000155         5756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8858_length_5756_cov_3.790388, whole genome shotgun sequence.
ACCESSION   DAOSCV010000155 DAOSCV010000000
VERSION     DAOSCV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8858_length_5756_cov_3.790388"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..364)
                     /gene="mraY"
                     /locus_tag="PLY56_05220"
                     /pseudo
     CDS             complement(1..364)
                     /gene="mraY"
                     /locus_tag="PLY56_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
     gene            383..1372
                     /locus_tag="PLY56_05225"
     CDS             383..1372
                     /locus_tag="PLY56_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20915.1"
                     /translation="MRGEAPANAAVTVNGTPMRRDGTTFVGNVVLRDFETKLTARAEG
                     AGEASITVLWDRNSFPRYRFSVDDNIQFLKDLAVNATRYNSLFDNWYLAFWREMHQRY
                     GARVQFNIYYATDERDFNLSQMPERYRDEWKANADWLRLTFHAYADHPDRPYLRATYE
                     EIERDFVLIHREIERFAGPELLSPFTTIHWGAATLEACRALRDHGMRGFAGYFILQDG
                     EPLVSYYLDREQVEHVNTRDYWKDMRENILFVKHDQVVNTIPLERVVPHLDEVAARPH
                     EAEVIELMVHEQYFCPFLSHYQPDIQQRVEAAIRWVTEKGYKPIFFEEGFLGA"
     gene            complement(1451..5284)
                     /locus_tag="PLY56_05230"
     CDS             complement(1451..5284)
                     /locus_tag="PLY56_05230"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HOJ20916.1"
                     /translation="MAVVFGIWATPSMARAASPASFAYQLHEETASGAYDVSASVRAS
                     GDGEARLCFGYRGPKDHDYVRVTRRAVSLVRVSGGKERLLASPYTLSVPLGPEATPVL
                     VKRRPEKVSVVFRGQLIQVAGDVSNAEGQVGIGATGAFRATDLRLQPVEPPSFSDDFM
                     REPSEAGPWEILEGTWGTTTVGDTEKSANGFFYRGSGTPGLAISGIPFWDDYILQASV
                     KCTKPDGAFGLGVYCQDAKNYLGLVWNVTNGLRLIRVVNGRTTVLATKPVAWVQGQWY
                     RLALQVLDGHLTAMVDGKVCLEARESLFSQGKIALYVEGEEIYFDDIAVKPAVYFRDA
                     DPMARRERWSRVSGGYITGSPSWQDYTYRVRTLPTDLNGVGLIFGWRDPENYYLFRWG
                     ANAGTAPGAGRQQLIRVAEGKRVVIADARGGYEPNRAYVIETAFRPGYAAVRIDGQRV
                     LETADPHLTHGAVGVTPFDSRVKEVTVTLREEPPPPEEEITKEFTDSAAHPDMAEWAN
                     PARAWVLRAGPKGPVFWRKGEYFGDVSVELTLPKHDPSQTLAFHLAARDRDPQSGYTI
                     VARTPEPGKLVLQLLRQAGTEPVAAVTAGIAPGTTPTLTLSYIGSYLLADLDNRRILK
                     YADPNPLRGSCVGLYVVGVSLDLRNVRVRSRNVYDYTFHDAPTDWEPQSGAWEVTSRW
                     SCSPGWSWFGGWSEGLAAIWNKRRFEGDFVLEYYAAPKMDAVAEAYAQRFRDLNATIC
                     ADGKRLSSGYSFLIGGWRNKRTAILRGDRVVAETRSYTLPPQKVGHRQWFYVRIEKIG
                     NRISLSVDDKHLLSYEDPDPLTGDRVALWTQNNGIMLARVLVCYEKEKELPLVTRKPG
                     KPETPATRLAAAASVPVTLPVFNDFETGVGQWRGVNARLATDATTPAAGKRCLAVTNP
                     QPGGDFRVQAIPGRFDAMQAKTLAFDYRVPPGILVNLYVRIGRIYYVIGFTGQPSVNP
                     AAGVLASLGKIEDVRADNAWHRAEFDLLGALRRFFPNANPLFVEELVFANWDSSDENM
                     YADAGLTGNAMGARYYIDNFVLGDMPAAALPQATPAPKPAAAIGPKAPQPVRVATRSA
                     PAIPEPRPESPTPAKPRVALLETFTGGKTTTTWDSMDGKWAVTRDGRFAPTEKPEFAF
                     AFTGDREWRDYALKADLINGQDAGLLVRAQDWNNCVFLVIRPGKRDLWWFVRRNGQWG
                     GVLAPMALPFGTKPLLKVKVLVTGDTYTCYLDGKKVSQIKDATFPKGRIGVYIHPAEP
                     GQAWDNVEVVLLGSS"
     gene            complement(5417..>5756)
                     /locus_tag="PLY56_05235"
     CDS             complement(5417..>5756)
                     /locus_tag="PLY56_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20917.1"
                     /translation="KPPPKLTGECTLTFKVPQDGTYSLWARAWWLDGCGNSFAVSVDG
                     GDRATISSGNYKRWHWVRGPKLRLTAGTHTLVLHNTEDGARLDQIFLTSDPARIPVGK
                     EKATPAALVK"
BASE COUNT         1001 a         1889 c         1863 g         1003 t
ORIGIN      
        1 gccgcttgcc cgtgcgcttg aacaccgcca cctgcaccat caccgaaagc gtttcgagca
       61 cgaacagacc gccgacgatc accagaacga tctcgtgctt tgtcgcgacg gccaccgaac
      121 caagcaaacc gccgagcgcg agcgaaccag tatcgcccat aaacacgcgc gcgggcggcg
      181 cgttgtacca gagaaagcca agcgaggcgc cgagcagcgc gcccagaatt gtcgaaagct
      241 cgcccacgcc tgcaacgtgc ggaatgccaa gatattgcga atagtcgacg cgcccgacga
      301 gatagcagat aacgccgaac gtgcccgccg cgatcatcac cggcacgatg gcaaggccgt
      361 cgagggcggg cttgagatta ctgtgcgcgg agaggcccct gccaacgccg cggtgaccgt
      421 caacggcact ccaatgcgcc gcgacggcac gacctttgtt ggcaacgtgg tcctgcgcga
      481 cttcgagacg aagctcaccg cgcgcgcgga aggcgccggc gaggcaagca tcacggtgct
      541 ttgggaccgc aactccttcc cgcgttatcg cttctcggta gatgacaata tccagtttct
      601 gaaggacctc gcggtcaacg ccacgcgcta caactcgctc tttgataact ggtacctcgc
      661 gttctggcgc gagatgcacc agcgctacgg cgcgcgcgtc caattcaaca tctactatgc
      721 cacggatgag cgcgacttca acctctcgca gatgccggag cgctatcgcg acgagtggaa
      781 agcgaatgcc gactggctgc gcctcacctt ccacgcctac gccgatcatc ccgaccggcc
      841 ctacctgcgg gcgacctacg aggagatcga gcgcgacttc gtgctgatcc accgggagat
      901 cgagcggttt gccggtccgg agctcctcag ccccttcacc acgatccact ggggtgcggc
      961 caccctggaa gcctgccgtg ccctccgtga ccacgggatg cggggcttcg ccggctactt
     1021 catcctgcaa gatggcgagc cgctggtctc ttactacctc gaccgcgagc aggtggagca
     1081 tgtgaacacg cgcgactact ggaaggacat gcgcgagaac atcctgttcg tcaagcacga
     1141 ccaggtggtc aacacgattc cgctggagcg cgtggtcccg cacctggatg aggttgctgc
     1201 acgcccgcat gaggcagaag tgatcgagct gatggtgcac gagcagtact tctgcccgtt
     1261 cctcagccat taccagccgg atatccagca gcgggtggag gcggcaatcc gctgggtgac
     1321 ggagaagggc tacaagccga tcttcttcga agagggcttc ctcggagcgt aacgatccac
     1381 gccgcacgtg gcgcgggccg gccggcccgc gcccgcatcc ccctcatccg ggggtattgt
     1441 caccgggctg ctaactggat cccagcagga cgacctcgac gttatcccac gcctggccag
     1501 gctcggccgg gtggatgtag acgccaatgc gccccttggg gaaggtggcg tctttgattt
     1561 gggagacctt cttgccatcc agatagcagg tgtaggtgtc gccggtcacc agaaccttca
     1621 ccttcagcag cggtttggtg ccgaagggaa gcgccatggg cgccagtacg ccgccccact
     1681 ggccgttccg gcgcacgaac caccacagat ctctctttcc ggggcggatc accaggaaga
     1741 cgcagttgtt ccagtcctgg gcgcgcacca agagaccggc gtcctggccg ttgatcaggt
     1801 ctgccttcag cgcgtagtcg cgccactccc ggtcgccggt gaaggcgaac gcgaactctg
     1861 gtttttcagt gggcgcgaac cggccgtcgc gggtgaccgc ccacttccca tccatcgaat
     1921 cccaggttgt tgtggtcttg ccgccggtga aagtctcgag gagggcgacc cgcggtttgg
     1981 cgggtgtggg agactctggc cgcggctctg ggatggccgg cgcgctccgc gttgccacgc
     2041 gcacgggctg cggcgccttg ggcccgattg ctgccgccgg tttcggcgcc ggagtggctt
     2101 gcggcagcgc ggcagccggc atatcgccca acacgaagtt gtcgatatag tagcgggccc
     2161 ccatggcgtt cccggtcagc ccggcatccg catacatgtt ttcgtcgctg gagtcccagt
     2221 tggcgaagac gagttcctcg acgaagagcg ggttcgcgtt ggggaagaag cggcgcagcg
     2281 cgcccaacag gtcgaactcc gcccggtgcc aggcattatc cgcgcgaacg tcctcaatct
     2341 tgcccaggct ggcgagcacg ccagcggcgg ggttgacgct cggttggccg gtgaatccga
     2401 tcacatagta gatccggccg atgcgcacat agaggttcac cagaatcccc gggggcacgc
     2461 ggtagtcaaa agcgagtgtc tttgcctgca tggcatcgaa ccgcccgggg atcgcctgca
     2521 cccgaaaatc gcctcccggc tgagggttgg tgaccgccag gcatcgcttt ccggctgccg
     2581 gggtagtcgc gtccgtggct aggcgcgcgt tcaccccgcg ccactgccca acgcccgtct
     2641 cgaaatcatt gaacaccgga agcgtcaccg gaacggaggc cgccgccgcc aatcgggttg
     2701 ccggcgtttc tggcttgcct ggcttgcgcg tgaccagcgg cagttccttc tccttctcat
     2761 agcaaaccag gacgcgggcg agcatgatgc cgttgttttg cgtccagagc gccacgcggt
     2821 cgcccgtcaa cgggtcgggg tcctcgtaag agagaaggtg tttatcgtct accgagagcg
     2881 agatgcggtt tccaatcttc tcgatgcgca catagaacca ctggcggtgc cccaccttct
     2941 ggggaggcag agtgtaggag cgcgtttcgg ccacgacgcg gtcgccgcgc aggatcgcag
     3001 ttcgcttgtt gcgccagcca ccgatcagga aggagtagcc gctgctcagg cgcttgccat
     3061 ccgcgcagat ggtggcgttc aggtcgcgga aacgctgggc gtaggcctcg gccacggcat
     3121 ccatcttggg cgccgcgtag tattccagca cgaagtcgcc ctcaaaccgc cgcttgttcc
     3181 agatggcggc taggccctcg ctccatccac cgaaccacga ccacccgggg ctgcaagacc
     3241 accggctggt cacttcccag gcgcccgatt gcggctccca atccgtgggc gcgtcatgga
     3301 aggtgtagtc gtagacgttc cgagagcgca cccgcacatt gcgaagatcc agcgagacgc
     3361 caaccacata gagcccgacg cagctgccgc ggaggggatt cggatccgcg tatttcagga
     3421 tgcgcctgtt gtccaggtcg gcgagcaggt agctcccgat gtaggagagc gtcagagtgg
     3481 gcgttgtgcc gggcgcgatt ccggcggtga cagcagccac gggctcggta cccgcctgcc
     3541 gtaggagctg cagaaccagc ttgccgggct cgggcgttcg cgcgacgatg gtgtagccgc
     3601 tctgtggatc cctatcgcgg gctgcgaggt ggaaggcgag ggtctggctc gggtcgtgct
     3661 tcggcagggt gagttccacg gagacatcgc cgaagtattc ccccttgcgc cagaagacgg
     3721 ggcctttcgg tccggcgcga agcacccagg cgcgtgccgg gttggcccac tccgccatat
     3781 cggggtgcgc ggctgaatcg gtgaactctt tggtgatctc ctcctcgggt gggggcggct
     3841 cctcgcggag ggtcaccgtc acctccttca cgcgcgaatc gaagggggtc actcccaccg
     3901 cgccgtgcgt caggtgggga tctgccgtct ctaggacgcg ctggccatcg atgcgcacgg
     3961 cggcataccc gggccggaac gccgtctcga tcacgtaggc gcggtttggc tcgtagccgc
     4021 ccctggcatc ggcgatgacc acgcgcttgc cctcggccac ccggatgagc tgttgtcggc
     4081 cggcgcccgg tgccgtgccc gcgttggcgc cccagcgaaa gaggtagtag ttctctgggt
     4141 cgcgccatcc gaagatgagt ccgaccccgt tcaggtcggt gggaagggtg cgcacgcggt
     4201 aggtataatc ctgccaggag gggcttcccg tgatatagcc tccgctgacg cggctccaac
     4261 gctcgcgccg ggccatcggg tcggcatcgc ggaagtagac ggccggcttc acggcgatat
     4321 catcgaagta gatctcttcc ccctcgacgt agagggcgat ctttccctgg ctgaagaggg
     4381 attcacgcgc ctccaggcag accttgccat cgaccatcgc ggtcaggtgg ccgtcgagga
     4441 cctggagggc gaggcggtac cactggccct gtacccaggc gaccggctta gtcgccagaa
     4501 ccgtggtgcg gccgttgacg acacggatca ggcggaggcc attggtgacg ttccacacca
     4561 atccgaggta gttcttcgca tcctggcagt acactcccag gccaaacgcg ccgtctggct
     4621 tggtgcactt gacggacgcc tggaggatgt agtcatccca gaaggggatg ccagagatgg
     4681 cgagaccggg agtgccagat ccgcggtaga agaagccgtt ggcgcttttc tccgtatcgc
     4741 cgaccgtcgt ggtgccccag gtaccctcca ggatttccca ggggccggcc tcgctcggct
     4801 cacgcatgaa gtcgtcggag aaggaggggg gctcgacggg ctgcaggcgc agatccgttg
     4861 cccgaaacgc accggtggca ccgatgccta cctgcccctc ggcgttgctc acatcgcccg
     4921 ccacctgaat gagctggccc cggaagacga cgctcacctt ctcagggcgg cgtttcacca
     4981 gcaccggtgt cgcctcgggg cccaggggaa cgctcagagt gtagggagac gccaggaggc
     5041 gctcctttcc tccactcacg cggaccagag acacggcccg gcgggtgacg cggacgtagt
     5101 cgtgatcctt gggcccgcgg tagccaaagc agaggcgcgc ttccccgtct ccagacgcgc
     5161 gcacggaggc gctcacgtcg taagcgcccg aggccgtctc ctcgtggagt tgataggcga
     5221 aagaggctgg ggaagcagct cgggccatgc taggggtggc ccagatcccg aagacgaccg
     5281 ccatggccag ccatctccga tgggcgcccc gtcccatgac ctcgagctgc agcggccgct
     5341 tccgcaccat tggaattctg aggtgcccgc gagcgtacgt gccgcccatt cgtccccttc
     5401 tcccttcaag catcacctac ttcacgagtg ccgcgggagt tgccttctcc ttgcctaccg
     5461 ggatccgcgc ggggtcactg gtcagaaaga tctgatccag gcgcgctcca tcctccgtgt
     5521 tgtgcagcac gagcgtatgc gtgccagccg tgagccgcag cttggggcct cgcacccaat
     5581 gccaccgctt atagttgccg ctgctgatcg tcgcgcggtc gccgccatcc accgagacgg
     5641 caaaggagtt cccgcatcca tcaagccacc acgctcgcgc ccacagcgag taggtgccat
     5701 cctgcggaac cttgaatgtg agggtgcact ccccggtcag ctttgggggc ggcttg
//
LOCUS       DAOSCV010000156         5753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8862_length_5753_cov_3.673921, whole genome shotgun sequence.
ACCESSION   DAOSCV010000156 DAOSCV010000000
VERSION     DAOSCV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8862_length_5753_cov_3.673921"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PLY56_05240"
     CDS             complement(<1..364)
                     /locus_tag="PLY56_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20918.1"
                     /translation="MVSLRLRPTKKVLIILGVAALASLGLSYFVLKAQARQRAEWEAK
                     LAAKMKEAEELESAALQLPDSEAALQDLQYRVSFLRGADEEEYQPALLTEMTELAWRT
                     GVEISGFNPVEGAPTAQSP"
     gene            complement(384..1097)
                     /locus_tag="PLY56_05245"
     CDS             complement(384..1097)
                     /locus_tag="PLY56_05245"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ20919.1"
                     /translation="MPSVNLIWEQRLVQRKHQRIATLLLAVIGIGLLVIAEEVVRTAM
                     KRRECVAGIQKCEERIKQARPLADRNRELQTQIAALEPTVALVKDAQRITLRWVGLMG
                     ELDASVPTPGPVYLTQLNFSTGEASAGKQVRPGTIGTLNLSGVARSYPQIAQMMRRLS
                     AQAHLREVRLLQAQALSEEGQIRFSCRAQFHIPEDEEARLAVNVEEQVAQRDYTAVVQ
                     RAGEKTVLHRTPLTDGETQ"
     gene            complement(1122..2363)
                     /gene="pilM"
                     /locus_tag="PLY56_05250"
     CDS             complement(1122..2363)
                     /gene="pilM"
                     /locus_tag="PLY56_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOJ20920.1"
                     /translation="MGLFGGSSIVGLDIGTSSIKAVRLQAAKNGWDIVAAARVETPRG
                     AVADGMIADERALLPAMHHLFAQIRARRSAVIAAVNFTSGVSVRRVGLPVLTPEALRK
                     QIQTQPDLFGLSPGTEQIHDFEILGTHGEGTAAQMDTLLVSAQRTAVECRLHLLESAG
                     LEPVAMDIEPFALMRSLVEVNPALSGLRGAIGILDLGAVHTDVTVVRGTNYIFTRAIP
                     LGGNSFTAVVQQALECTLEKAEKLKTEIDLNWLLQGPGEQADTPAYQATAAIKSVLDD
                     LLREVRNSILFYQSQLPDDDVEDGMVAMILLAGGTALMKGLPEYMQAAFSLQVQRADC
                     SWSLPYRIALRDGNLGPEESPLYALAAGLAAREPVEQARRETANGGKRRARGKKADAE
                     PAPAQVEEPAQPDEPGDEASQ"
     gene            complement(2496..3584)
                     /locus_tag="PLY56_05255"
     CDS             complement(2496..3584)
                     /locus_tag="PLY56_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOJ20921.1"
                     /translation="MINLQALFDEADRRNVADIYLKVGSPAMIRAEGRTLPFDDHVLA
                     AEETRAVALEIMGEARMREFEKTQEMNLAYSVPGVGRFRCNAFMQRGTVAMVMRRVKD
                     DVPTLEELNLPKQLAKLVMEERGLILVTGATGSGKSTTLAAMIDYRNTHAEGHILTLE
                     DPVEFLHRDKKSIVCQREIGTDTESFAQALKNALRQTPDVILIGEMRDRESVKAALDF
                     AETGHLVLSTLHSNNANQTIERVLQFFPAEEHPQILHHLANNLRGIISQRLLRKADGS
                     GRVAAIEIMIATQRIKDILRSGELIKLKATIEQGQNEGMQTFDQHLYQLHKVENVISL
                     EDALAAADSPNDLRLRIRGLSSGGLTVS"
     gene            complement(3638..4768)
                     /locus_tag="PLY56_05260"
     CDS             complement(3638..4768)
                     /locus_tag="PLY56_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ20922.1"
                     /translation="MDIAELLMLTVEKNGSDLHLNVGAPPCIRVHGEMEPQEYPELTK
                     DDIHGMLYDIMTDAQKSRFEETRDLDFSVELGGVGRFRVNVLMTRRGEGAVFRTIPTK
                     IKSVDELGLPSVCRELTKPERGLVLVTGPTGSGKSTTLAAMIDLINETRRGHILTIED
                     PIEFVHQHKKCLVTQREVGPHTSSFATALRAALREDPDVILVGEMRDYETISLAVTAA
                     ETGHLVFGTLHTSSAHQTIDRIIDVFPEAQQNQIRTQLAESIQGVLSQALLRTADGKG
                     RVCAVEVMVGVPAVRNLIREGKTFQIPSILQTGQSDGMQTMDSCLINLVKSGKVHRDE
                     ALPLVTDKDTFNKQVGMGGAPSAGMGIPMGAPAMGLNLPPRR"
     gene            5081..>5753
                     /locus_tag="PLY56_05265"
     CDS             5081..>5753
                     /locus_tag="PLY56_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009439488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20923.1"
                     /translation="MIDVTKLFCGRDTTGDPIRYGHRRGPAHGNPHPTEGPAHAAPPS
                     ASDRRPVVAWNICRRCNLACVHCYTDSANRHYPGELTTEEGMALIDDLADFRIPALLF
                     SGGEPLIRPDLFTLAAHARARNLRITLSTNGTLIDRPLAQRIAIREVFLRQELVDHRH
                     RRAVGAVAFVEAAPEQQRDTHGLEIAWIDDVQVAVRTGRAWRSFATGDLVGRRPVLVA
                     ERQVAD"
BASE COUNT         1007 a         1950 c         1710 g         1086 t
ORIGIN      
        1 ccggcgattg ggccgtgggc gccccttcca cggggttgaa tccggagatc tccacgcccg
       61 tgcgccacgc cagttcggtc atctccgtca agagggccgg ctggtactcc tcttcatccg
      121 cgcctcggag gaagctcacg cggtattgca gatcttgcag cgcagcctcc gaatcgggaa
      181 gctgcaaggc ggcagactcc aactcctccg cctccttcat cttcgcggcg agcttcgcct
      241 cccactcggc acgctgcctg gcctgcgcct tgagcacgaa gtaggaaagc ccgaggcttg
      301 ccagcgcggc aacccccagg atgatcagaa cctttttggt cggtctcagg cgcagactca
      361 ccatttcccc ctgccggcga ctgctactgc gtctccccgt cggtcagggg agtgcggtgg
      421 agcaccgtct tctcccctgc cctctgaacg acggcagtgt aatctcgttg tgccacctgc
      481 tcctcgacat tcaccgccag ccgggcctcc tcatcttccg gaatatgaaa ctgggcccgg
      541 caggagaagc gtatctgccc ctcttccgat agcgcctgtg cctgcagaag ccgcacctcg
      601 cgcagatggg cctgcgccga caggcgcctc atcatctgag cgatctgggg atagctccgg
      661 gccacccccg acaggttcag cgtgccgatg gtgccaggcc gcacctgctt cccggcgctc
      721 gcttcccccg tggagaagtt gagctgggtg agatatactg gccccggagt cggcacactg
      781 gcgtcgagct ctcccataag gcccacccaa cgcagcgtaa ttcgctgggc atctttgacc
      841 agggccacgg tcggctccag ggcggcgatt tgggtttgaa gctcgcggtt ccgatccgcc
      901 agtggccttg cctgtttgat tcgctcctcg cacttctgaa tgccggccac gcactcccgg
      961 cgcttcatgg ccgtgcgcac gacctcctca gcaatgacca gaagaccgat gccgatcacg
     1021 gccagcagca acgtggcaat gcgctggtgc ttccgctgga cgagccgttg ctcccatatc
     1081 aggttcaccg acggcatgcg ctactcccac ccgcgtccat cctactggct cgcttcatcg
     1141 ccgggctcat cgggctgtgc cggctcttct acctgggcag gcgccggttc cgcgtctgcc
     1201 ttctttcccc gggcgcgccg cttaccgcca ttggcggtct cccgccgtgc ctgctcgaca
     1261 ggctcccgag ccgccaatcc tgcggccagg gcatagaggg gcgattcttc cggtccgagg
     1321 ttcccatcgc gcaaggcaat gcgataggga aggctccagg agcaatcggc gcgttgcacc
     1381 tgaagagaga acgccgcctg catatactcc ggcaggccct tcatcagcgc tgttcccccg
     1441 gcaaggagga tcatcgccac catcccatcc tccacgtcat catccggaag ctgggactgg
     1501 tagaagagga tcgagttgcg gacttctcgc aggaggtcat ccaacaccga tttgatcgcc
     1561 gccgtcgcct gatacgccgg ggtatcggcc tgctcgccgg gcccctgaag cagccagttc
     1621 aggtcgatct cggtcttgag cttctccgcc ttctccaggg tgcactcaag ggcttgctgt
     1681 accaccgcgg tgaacgaatt accccccagt gggatggcgc gcgtgaagat gtagttcgtg
     1741 ccgcgcacca ccgtcacatc ggtatgcacc gcacccaggt cgagaatgcc gatagcgccc
     1801 cgcaatccgg agagcgccgg gttgacctct accagggagc gcatcaacgc gaacggctcg
     1861 atatccatcg ccaccggctc gaggccggca ctctcgagga ggtgcagccg gcattcaacg
     1921 gcggtgcgct gcgcggagac gaggagcgta tccatctgcg cagccgtgcc ctcgccgtgc
     1981 gttcccaata tctcgaaatc gtggatctgc tcggtccccg ggctgaggcc gaacaggtct
     2041 ggttgcgtct ggatctgctt gcggagggcc tcgggagtga gcaccggcag ccccacccgg
     2101 cgcaccgaca ccccggacgt gaagttgact gcggcgatca ccgcggaccg gcgcgccctg
     2161 atctgggcaa agaggtgatg catcgcgggc aacagggccc gctcatcggc aatcatcccg
     2221 tcggcaacgg cgcctctcgg cgtttccact cgcgcggctg cgacgatatc ccagccgttc
     2281 ttggccgcct gcagccggac cgccttgatg ctgctggtgc cgatgtccag accgactatc
     2341 gagcttccgc cgaacagacc catgccatcc ccaatccggg ccattgccgc aagctacccg
     2401 gacgaactcc ccgccccacg agtgcgcccg ccaccctgtc ggggagcggc aggaagcgtg
     2461 ttggcgctcc ctccatgttc aaggcttcgc cttccctacg atacggtcaa gcctcctgaa
     2521 gagaggccac ggatccgcaa gcggaggtcg ttgggactat ccgcagcagc cagggcatct
     2581 tcaaggctga tcacgttttc gaccttgtgc agttggtaga ggtgctggtc aaacgtctgc
     2641 atcccctcgt tctggccctg ttcgatggtt gctttcagtt tgatcaactc gccgcttctc
     2701 agaatatcct taatgcgctg agtggcgatc atgatctcaa tcgcggcgac gcggccagag
     2761 ccatcggcct tgcgcaataa ccgctgggag atgatgccgc gcagattatt ggcaagatgg
     2821 tgcaggatct ggggatgctc ttctgcgggg aagaactgaa gcacgcgctc gatcgtttga
     2881 ttggcgttgt tggagtggag cgtgctgagg accaggtggc ctgtctccgc aaaatcgagg
     2941 gccgctttga cgctctcgcg gtcgcgcatc tcgccgatga ggatcacatc tggcgtctga
     3001 cgcagggcat tcttgagtgc ctgagcgaac gactcggtat cagtgccgat ctcacgctgg
     3061 cagacgatgc tcttcttatc ccgatggaga aactccaccg ggtcctcaag ggtgaggata
     3121 tggccctcgg cgtgggtgtt ccggtagtcg atcatcgcgg cgagcgtggt cgactttccg
     3181 gagccggtcg cgcccgtcac caggatgagc ccgcgctctt ccatcaccag cttggcgagc
     3241 tgcttgggca gattcagctc ttccagcgtg ggcacatcat ctttcacccg gcgcatcacc
     3301 atcgccacgg tgccgcgctg catgaaggcg ttgcagcgga agcggccgac cccaggaacc
     3361 gagtaggcca ggttcatctc ctgggtcttt tcgaactcgc gcatccgtgc ctcgcccatg
     3421 atctcgagcg cgaccgcccg tgtctcctcg gcggccagca catggtcatc aaagggaagc
     3481 gttcggcctt cggcccggat catcgccgga gaacccacct tcaggtagat atctgcgacg
     3541 ttacggcggt cggcctcatc gaaaagcgct tgcaggttga tcacgcccac attctcctat
     3601 gacgtgttcc gctccccaga tccctggccc gagaatccta gcgtcgcgga ggcaggttca
     3661 atcccatcgc gggggcgccc atcggtatgc ccatgcccgc actcggagcg ccgcccatgc
     3721 ccacctgctt gttgaaggta tccttatccg tcacgagtgg caacgcctca tcgcgatgca
     3781 ccttcccgct cttcaccagg ttgatgaggc agctatccat cgtctgcatc ccatccgatt
     3841 ggccggtctg caggatcgac gggatctgga aggttttccc ctcacgaatg aggttgcgca
     3901 ccgccggcac gcccaccatc acctcaaccg cgcagacgcg gcccttgcca tcggcagtgc
     3961 gcagaagcgc ctgcgacagc acgccctgga tcgactccgc gagctgagta cggatctggt
     4021 tctgctgcgc ctccggaaaa acgtcaataa tccggtcgat ggtctgatgc gccgagctgg
     4081 tgtgcagcgt gccaaacacc aagtggcccg tttcggcagc ggtcaccgca agagagatgg
     4141 tttcataatc gcgcatctcg cccacgagga tcacatcggg gtcctcgcgc agagcggcgc
     4201 gcaacgcggt ggcgaaactg gatgtatgcg ggcctacctc tcgctgggtc accaggcact
     4261 tcttatgttg gtggacaaac tcgatggggt cttcaatcgt caagatatgt ccccggcggg
     4321 tctcgttgat caggtcgatc attgcggcca gggtggtcga ttttccagag cccgtcggcc
     4381 cggtcaccaa taccaggccg cgttccggct tcgtcagctc ccggcacaca ctggggaggc
     4441 ccagctcatc caccgactta atcttggtgg gaatggtgcg gaaaacggcg ccctcgccac
     4501 ggcgggtcat caacacattc acgcgaaacc ggcccacgcc ccccagctcg acggagaagt
     4561 ccaggtctcg cgtctcctcg aagcggctct tttgggcatc cgtcatgata tcgtagagca
     4621 taccatggat atcatccttg gtaagctcgg gatactcctg gggctccatc tcgccatgaa
     4681 cacggatgca aggaggcgcc cccacattca gatggaggtc agaaccgttc ttctcgacgg
     4741 tcagcatcaa cagctctgcg atatccactc gggtatctcc ttgccgcacc aatcgagggc
     4801 aaccggccaa accgcctggg gacaacacac tcgcctaata ggcagaccca ctatagcacg
     4861 cgcgcgcgca ggttgtcaag aaaccaggca acgagatcga agccggtgcc tgccctcgcg
     4921 ctgttgcgca ggatcctctc ccggagcggc gaagaatcgt gagagaggcg tgccacgccc
     4981 cgcggtgcct ggtgaacccc gccgccagga cgccctggtc gataccgcgc ctgctgcccc
     5041 cgcgagcaaa tacacctgcg caacgcctgg gagaaagccc ttgatcgacg tcacgaaact
     5101 tttctgcggc agagacacca ccggcgaccc gatccgctac ggtcaccgcc ggggtccggc
     5161 gcacgggaat ccacacccca ccgagggccc cgcgcacgcg gcgcctccct cggcctcgga
     5221 ccggcgcccc gtcgtcgcgt ggaacatctg ccggcgctgc aatctggcgt gcgtgcattg
     5281 ctacaccgac tccgccaacc ggcactaccc aggggagctg accacggaag agggcatggc
     5341 gctcattgac gacctggccg attttcggat cccggcactc cttttctcgg gcggggagcc
     5401 gctgatacgg cccgaccttt tcacgctggc ggcccatgcc cgcgcccgga acctgcggat
     5461 caccctctcg accaacggca cgctgattga tcgcccgctg gcccagcgca tcgccattcg
     5521 ggaagtgttt ctgcgccagg aactggtcga tcaccgccac cggcgcgccg tcggcgcggt
     5581 cgcgttcgtc gaagctgcgc ccgagcagca gcgggatacc catggccttg aaatagcctg
     5641 gatcgatgat gtgcaggtag ccgtgcggac tggccgtgcc tggcgcagct tcgcgaccgg
     5701 cgatctggta ggacgccgtc cagttctggt tgctgaaagg caggttgctg acc
//
LOCUS       DAOSCV010000157         5752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8866_length_5752_cov_3.868878, whole genome shotgun sequence.
ACCESSION   DAOSCV010000157 DAOSCV010000000
VERSION     DAOSCV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8866_length_5752_cov_3.868878"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLY56_05270"
     CDS             <1..670
                     /locus_tag="PLY56_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20924.1"
                     /translation="YRVAFTRADSSPHRKTIHLTVTRGDATITASPTMRFSPQMGSWV
                     HAPAVHRSWQQDLYISPLDYRDREEAATKRADLGRGETMESGSYAFTFEGFDMSEAHA
                     GAGILIGVRLPSGKRVRVTPTLRLGEGGDLVPTPAKLPGTEVAVAVTDLSVPMDRPRE
                     SRITLSVTGLPIESGSSPEESVVVEVSIKPLINLLWLGSVLLLAGGVMAGIRRAKEAQ
                     REAS"
     gene            834..2378
                     /gene="xylB"
                     /locus_tag="PLY56_05275"
     CDS             834..2378
                     /gene="xylB"
                     /locus_tag="PLY56_05275"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOJ20925.1"
                     /translation="MAYLLGIDVGTSGTKALVISEEGVPLGSATADYPLSTPQPGWAE
                     QNPADWWRATAVAIRGACERAGIAPEAISGIGLSGQMHGSVFLDDAHQVIRPAILWCD
                     QRTAAQCERITERVGRDHLVRITCNQVLTGFTAPKIVWLQENEPEAYARVRKVLLPKD
                     YIRFRLTGEFATEVSDASGTSLFDVPRRCWSGEILDALGIPESWMPRVYESPEISGRI
                     HATAAAETGLKEGTPVVGGGGDQAAGAVGSGIVSPGAVSISLGTSGVVFAYADEPATD
                     PELRTHTFCHAVPGKWHVMGVMLSAGGSLRWYRDHLAQHEVEVATRLEVDPYELICAE
                     AARAPLGSEGLLFLPYLTGERTPYPDPHARGVFFGLSLRHDRRHMARAVIEGVSFGLR
                     DSFEILKAMGVPIGQVRVSGGGARSAIWREVLAATLNTPLFQATTEEGAAFGAALLAG
                     VGLGLYPSVPEACARTIAAREVTPAPPAVEVAAYNAYHRIYRQLYAQLKDLFDETARI
                     VQEEQA"
     gene            2375..3196
                     /locus_tag="PLY56_05280"
     CDS             2375..3196
                     /locus_tag="PLY56_05280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ20926.1"
                     /translation="MTNLNQRHPQFTEVAAAYDALMSGVPYKRWVDYIEKLLRRLRAS
                     PRTVLDIACGTGNVTFALADRGYQVAGVDISEPMLSVAREKARQQGRDIPFMHGDMRT
                     LSLPDRYDLAVCLYDSINYLLTLEDVTQAFASVHRVLHPGGLYIFDVNTIYALRADLF
                     TQRNLDPGARVRYDWRSKYNHETRICEVVMDFWVRENGETRHFTEVHYERGYFLRELV
                     DALTQVGFRTLAVYEAYTFHEPRLLSDRVYFVARKQRARKESDAGEMVVPCLDGI"
     gene            3178..5481
                     /locus_tag="PLY56_05285"
     CDS             3178..5481
                     /locus_tag="PLY56_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ20927.1"
                     /translation="MSGWNLSSHRGVRRAAVKPVRAANARRRAGRAVLWGVPVLFLLL
                     LAATSGRAQDAGAMIPTPQPDPAQPAAEAIVPLETEAPALPLLPASPAASRVLGVVDK
                     PELVFLLQKAGLDARPLSYAQLEVGALDLFGLLMIDLEKPLSVAAAAFVSDFVARGGR
                     LIACSWGAAVAPRRQAAFPAYRLLEALQVRVTGWSADGNAYLRAGDAAPLFRALPSFL
                     DVRIRATPLLEPLASGRVGAYWVSEDLSRRGGSDVRSPAIVLAPRCAYFAPNVLAEAL
                     HSRQMLRLLINAISHLVPGLAPDPARLALAELEVVLGEARRVAAISQDPDAPGLLAVA
                     EGKSVTAQALFTQPAGPLDATAALQPPVTGALSPEGEAGATGNGVVDAASAGAPEAAA
                     QEGTSAPAVPSPDAGQTLAAIAEAIAASERVALLQIPCRTVEARGVLLPRRVLPPSRF
                     AIGKLLDSLQAAGINLVFAEVYSAGQTLSPGPHQDPRFAGHNPLASLIAEAGPRGIAV
                     HAWISLLTAGAPGQKGGILSTNARWAAQTRNGGRYVSGGEQWLCPSQVEPRDTVVQRI
                     QQALANVPIQGVLLDGVDYGGLPEACYDPNCVALFRSDTGRNPREALKGEWEQEWLRW
                     RRGRIDTLVRRVARAVRAQHPTVPLHLAVTAASADPRNNSLADWRAWIREGWIDGLCP
                     HSGTGIAPTARKSTERVLRLAPGTRVMPIVDTARVRTARHAMTLVTALQEGGATGILF
                     DLPQPITGAWCAELGRGSFRRTAALPW"
     gene            5519..>5752
                     /locus_tag="PLY56_05290"
     CDS             5519..>5752
                     /locus_tag="PLY56_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20928.1"
                     /translation="MNATLRALNRPLLPGARVYLVGGCVRDLLLGRPIRDLDLVTDGN
                     PRRLASMVARRTGGAPFLMHAELRAYRIAPRGST"
BASE COUNT          864 a         1975 c         1953 g          960 t
ORIGIN      
        1 ctaccgcgtc gccttcaccc gtgccgactc ctcgccacac cgaaagacca tccacctcac
       61 tgtcacccgc ggcgatgcca ccatcaccgc gtctcctacc atgcgcttca gcccgcagat
      121 gggctcgtgg gtccacgcgc cggcggtgca ccggagctgg cagcaagacc tctacatctc
      181 gcccctcgac taccgggata gggaagaggc agccaccaaa cgcgcggatc tgggccgggg
      241 ggagacgatg gagtccggca gctacgcctt caccttcgag ggcttcgaca tgagcgaggc
      301 gcacgcgggc gcgggcatcc tcatcggcgt ccggctgccc tccgggaagc gcgtccgggt
      361 cacccccacg ctgcggcttg gcgaaggggg cgatctggtg cccacgccgg cgaagctgcc
      421 tggaacggag gtcgccgtcg cggtgacgga tctcagcgtg ccgatggacc gacccaggga
      481 atcgcggatc acgctctccg tcaccgggtt gccgatagag agtggctcct cacccgagga
      541 gagcgtggtg gtggaggtga gcatcaagcc gctgatcaac ctgctctggc tgggctcggt
      601 gctcctcctc gccggcgggg ttatggcggg catccgccgg gcgaaagaag cccaacgcga
      661 agcctcctga tgctggcgtg aagcccaggc ggtgccacgc gcgggccagc ccatcctctt
      721 ctcccgctcg caccgcgggt cctccagagg cgagccccag gtcgcaggaa caacgcggtt
      781 ggggagggaa gaggaaaagt ggagaaggca ttgaggcatc tggggagaag gcgatggcat
      841 acctgttagg catcgacgtg ggaaccagcg gcacgaaggc gctggtgatc tccgaggagg
      901 gggttccgct ggggtcggcg acggctgact acccgctctc cacgccccag ccgggttggg
      961 cggagcaaaa tccggccgat tggtggcgcg ccactgctgt ggccattcgg ggcgcgtgcg
     1021 agcgcgccgg cattgccccg gaggcgatct caggcatcgg tctctccggg cagatgcacg
     1081 gttccgtctt cctggatgac gcccaccagg tgattcgccc ggcaatcctg tggtgcgacc
     1141 agcgcaccgc cgcgcagtgc gaacggatca cagagcgcgt gggccgcgat cacctggtgc
     1201 ggatcacatg taaccaggtg ctgacgggct tcaccgcgcc gaagattgtc tggcttcaag
     1261 agaacgagcc ggaagcctac gcgcgcgttc gcaaggtgct cctgcccaag gattatatac
     1321 gcttccggct caccggcgag tttgccacgg aagtctcgga tgcgtcgggc acctcgctct
     1381 ttgacgtgcc gcggcggtgc tggtccggcg agatcctcga tgcgcttggc atccccgaga
     1441 gctggatgcc ccgggtgtac gagtcgcccg agatcagcgg gcgcatccac gcgacggccg
     1501 ccgcggaaac tggcctaaag gagggcactc ccgtcgtcgg tggcggcggg gatcaggccg
     1561 cgggcgcggt gggcagcggc atcgtctcgc caggggcggt ctcgatctcg ctgggcacct
     1621 ccggggtggt tttcgcctat gccgacgagc cggcgaccga tccggagctg cgcacgcaca
     1681 ccttctgcca tgccgtgccc ggaaagtggc atgtgatggg ggtgatgctg agcgccggcg
     1741 gctcgctccg ctggtaccgc gatcacttgg cgcagcacga ggtggaggtg gccacgcgcc
     1801 tggaagtcga tccctacgag ctgatttgcg cggaggcagc ccgggcgcca ctcgggtccg
     1861 aagggctcct cttcctcccc tatctcaccg gcgagcgcac accctatcct gacccacacg
     1921 cgcgcggcgt cttcttcggg ctgagcctgc gccatgaccg gcgacacatg gcgcgcgccg
     1981 tcatcgaggg agtctcgttt ggattgcgcg actcgtttga gattttgaag gcgatgggcg
     2041 tgcccatcgg ccaggttcgc gtctccgggg gcggcgcgcg cagcgccatc tggcgcgagg
     2101 tgcttgccgc cacgctcaac acgccgctct tccaggccac caccgaggag ggcgctgcgt
     2161 tcggcgccgc gctcttggcc ggggtcggcc tcgggctcta tccctccgtg ccggaggcgt
     2221 gcgcgcgaac catcgccgcg cgcgaggtga cgccagcacc gcccgcggtg gaggtggccg
     2281 cctacaatgc ctaccaccgg atttaccggc aactctacgc gcaactcaag gatctcttcg
     2341 acgagacggc gcgcattgta caggaagagc aggcatgacc aatctgaatc agcgacatcc
     2401 ccagttcacg gaggtagccg cggcgtatga tgccctcatg tcgggagtgc cctacaagcg
     2461 gtgggtggat tatatcgaaa agctgctccg gcggctgcgt gcctcgccgc gcaccgtgct
     2521 cgacatcgcc tgcggcacag gcaacgtcac gtttgccctg gccgaccgtg gttaccaggt
     2581 ggccggagtg gacatctccg agccgatgct cagcgtggct cgcgagaaag cacgccagca
     2641 aggccgcgac atcccgttca tgcatgggga tatgcgcacc ctctcgctgc ctgatcgcta
     2701 tgacctcgcc gtttgcctct acgatagtat caactacttg ttgacgcttg aggatgtgac
     2761 gcaggcgttt gcttccgtcc atcgcgtgct ccatccgggc gggctctaca tcttcgatgt
     2821 caacacgatc tacgcgttac gcgccgatct cttcacccag cgcaacctgg atccaggcgc
     2881 gcgcgtccgt tacgactggc ggagcaagta taaccacgag acgcgcatct gcgaggtggt
     2941 gatggacttt tgggtgcgcg agaacggcga gaccagacac tttacggagg tgcactacga
     3001 gcgtggctat ttcctgcggg aactcgtgga tgccctgacg caggttggct tccgcacgct
     3061 ggcggtctac gaggcctaca cctttcatga gccccggctt ctctcggacc gcgtctactt
     3121 tgtcgcccgt aagcagcgag cgcgcaagga atcggatgcg ggggagatgg tggtcccatg
     3181 tctggatgga atctgagtag tcatcgtgga gtgcggcgcg cggcggtgaa gccggtccgc
     3241 gcggccaatg cccggcgcag agcaggtcgg gccgtcctct ggggcgtacc cgtgcttttc
     3301 cttctcctgc tcgccgccac gtcaggaaga gcgcaggacg ctggcgccat gataccgacg
     3361 ccgcaaccgg atccggcaca gccggcggcg gaggcgattg tgccgcttga gacagaagcg
     3421 ccggcgctgc cgctcctgcc ggcgtctccg gccgcatcgc gcgtgctggg cgtagttgat
     3481 aagcccgagc tggtcttcct gcttcagaag gcgggcctcg atgcccgtcc gcttagctac
     3541 gcgcagctgg aggtgggcgc gctcgatctc ttcgggctgt tgatgatcga cctggagaag
     3601 ccgctgtcgg tggcggccgc cgccttcgtc tccgatttcg tggcgcgggg cgggcggctg
     3661 atcgcctgct catggggcgc cgccgtggct ccccggcgcc aggccgcttt cccggcctac
     3721 cgcctgctgg aggcgcttca ggtgcgcgtc accggctgga gcgcggatgg aaacgcctac
     3781 ctgcgtgccg gcgacgcggc gccgctcttc cgggcgctcc catccttcct ggatgtgcgc
     3841 atccgcgcga ctccactcct agagccgctg gcgtcaggac gtgtcggcgc ctattgggtc
     3901 tccgaggatc tttcgcgccg cggtggctcc gatgtgcgct cgcctgccat cgtgcttgcg
     3961 ccccgctgcg cctacttcgc gcccaacgtg ctcgccgagg cgcttcactc ccggcagatg
     4021 cttcggttgc tgatcaacgc gatcagccat ctcgtgcccg gccttgcccc ggacccggca
     4081 cgcctggcgc tggcagagct ggaggtggtg ctgggcgaag cccggcgcgt ggctgccata
     4141 tcgcaggatc cggatgcccc cgggctcctt gccgttgccg aggggaagtc cgtgacggcg
     4201 caagccctct tcactcaacc cgccgggccg ctcgacgcca cagccgcctt gcaaccgccc
     4261 gtgacaggcg ccctgtcgcc agagggcgag gcgggcgcca ctggcaacgg ggtggtggat
     4321 gccgccagcg ccggtgcccc cgaagccgct gcgcaggagg gcacatcagc gccggctgtt
     4381 cccagcccgg acgcagggca gacgctcgcg gcgatcgcgg aggcgatcgc cgcctcagag
     4441 cgggttgctc ttctccagat cccctgccgc accgtggagg cccgcggcgt cctgctcccg
     4501 aggcgcgtcc tgccgccctc gcgctttgcc attggcaagc tcctcgactc gctccaggcc
     4561 gccggcatca acctcgtctt cgccgaggtc tactccgccg ggcaaacgtt gagccccggt
     4621 ccgcaccagg acccacgctt tgccgggcac aacccgctgg cttcgctgat cgctgaagcc
     4681 ggcccgcgcg gcatcgcggt acacgcctgg atatcgctgc tcaccgcggg cgcgcccggt
     4741 cagaaaggcg gaatcctttc tacgaacgcg cgctgggctg cgcagacccg caacggcggg
     4801 cggtatgtct caggcggcga gcagtggctt tgcccctcgc aagtcgagcc gcgagacacc
     4861 gtggtccagc ggattcaaca ggctctggcg aacgtgccga ttcagggggt gctcctggac
     4921 ggggtcgatt acggcggcct gcccgaggcg tgctacgacc cgaactgcgt ggccctcttc
     4981 cgctcagaca ccgggcgcaa tcctcgcgag gcactgaagg gcgagtggga gcaagagtgg
     5041 ctgcgctggc gacggggacg catcgacacg ctggtgcgcc gtgttgcgcg cgccgtgcgc
     5101 gcccaacacc ccacggtgcc gctccacctc gccgtcacgg cagcctccgc ggatccgagg
     5161 aacaactcgc tggccgactg gcgcgcctgg atccgcgagg ggtggatcga cggcctctgc
     5221 ccgcatagcg gcaccggaat cgcgcccacc gcacgcaaga gcacggagcg cgtgctgcgc
     5281 ctggcgcccg gtacgcgcgt catgccgatc gtagatacgg cccgcgtgcg cacggctcgc
     5341 cacgccatga ccctggtaac cgcgctgcaa gagggcggcg ccacaggcat cctcttcgat
     5401 ctgcctcaac cgatcaccgg cgcctggtgc gccgagttgg gccggggcag cttccgacgg
     5461 acggcggccc tcccatggta aaggctggcc tgggcacccg tccgatagga ggccggtcgt
     5521 gaacgccacc cttcgggctc tcaaccggcc cctccttcca ggcgctcgtg tctacctggt
     5581 tggcgggtgc gttcgcgatc tgcttctcgg acggccgatc agggacctgg acctggtcac
     5641 cgatgggaac ccgaggcggc tggcctcgat ggtcgcccgc cggacgggtg gcgcgccctt
     5701 cttgatgcac gcggagctgc gcgcctaccg catcgcccct cgtggcagca cg
//
LOCUS       DAOSCV010000158         5737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8894_length_5737_cov_3.245512, whole genome shotgun sequence.
ACCESSION   DAOSCV010000158 DAOSCV010000000
VERSION     DAOSCV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8894_length_5737_cov_3.245512"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="PLY56_05295"
     CDS             complement(<1..1539)
                     /locus_tag="PLY56_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOJ20929.1"
                     /translation="MSSQGGQVVYTNKAHCRDCYRCLRVCPVKAIGMDDGQAYVVAER
                     CISCGTCIRECPQGAKAFRNDVDQAARLIASGVPVGASIAPSFASAFTEWERKCLPSA
                     LRRLGFAYVGETAIGAYHTALRTREIVDARPNRSHITTACPVVVNYIERYRPDLLDLM
                     IPVVSPMLAHARHIKERLGHGSRVVFIGPCVAKKAEAERPENAGVVDCVLTFTELEEW
                     FEREGIQLAQCEESHFDEEPEGESRYFPLVGGILRTAHMETDVLAPQTISASGFDELR
                     ESLEVLKPSGLPMIIEPLFCHQGCINGPAMPTRANAYARRQVLLGYATSARGRTPQPY
                     TDTHGLDTRFAAQPVLDAEGVSEEQIRAVLEQTGKANPENQLNCGACGYATCRDKAIA
                     VVRGMAEPGMCIPYMRRLAEQRTDRIIETSPNGIVILDEHLRILSMNPAFRRFFMCSE
                     AVCGRPISYLMDAEPFEQLATGKTDLIETTVKHARHNLVCHQIQYALREERQYVGIFV
                     NITRT"
     gene            complement(1652..2884)
                     /gene="hydF"
                     /locus_tag="PLY56_05300"
     CDS             complement(1652..2884)
                     /gene="hydF"
                     /locus_tag="PLY56_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOJ20930.1"
                     /translation="MNNQAPKGMRLHIGLFGRTNVGKSSLLNAITQQNVAIVSAEPGT
                     TTDPVEKAMELLPLGPVLFIDTGGIDDRATLGAERVARTMKAIDRTDLAILVTEPDGW
                     GDAEEGLLHRLQERQVPVMVVVNKRDQGPTAPALREHLERAKIPFAEISAKDAPQETA
                     ALIKEKLLQLAPEEWINSRPIVCDLLQRGDTVILVVPLDEEAPKGRLILPVQQVLREC
                     LEYGVRSYVIGPDQVAESLDQLREPPRLVITDSQAFATVMPATPPEIPLTSFSILFAR
                     FKGDLDALAQGARALMELTPESKVLIAEACTHHPFGEDIGRVKIPRLLRNRVGAGLTI
                     DVASGKEFPEGLADYDVVIHCGGCVLNRRGMLSRILRCREQGVPVTNYGMAIAAALGN
                     LDRVLRPFETLRDARPAA"
     gene            complement(2930..4429)
                     /gene="hydG"
                     /locus_tag="PLY56_05305"
     CDS             complement(2930..4429)
                     /gene="hydG"
                     /locus_tag="PLY56_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOJ20931.1"
                     /translation="MSAQLVEPKPTWASQVIKRDEVTRYLQDGRDFIDDAALEAQLAS
                     AKPDPVRVRDILQKSLAIETLTTEETATLLSVSDEALWDEMYDVAAQVKKKVYDNRVV
                     TFAPIYCSDLCVNNCLYCGFRAGNSGIKRRCLTMEEIAREAEVLAGQIGHKRLILVFG
                     EHPRTDARYIAEAMRTVYAVEVPTRTGSSTGIRRLNINAAPMSIDDLRLLKEVGLGTF
                     QVFQETYHHDTYAKLHPNGTVKSDYAWRITCMHRAMEAGVDDVGIGALFGLYDWRFEV
                     MGLVAHAHELEARFGIGPHTISFPRLTPALDTPFVQETRYRVSDKEFKRLVCVIRLSV
                     PYTGMIITVRERPDVIRECIPMCTQRDASTRIGVGAYQDEYEQQEADRQQFTLGDTTS
                     LDSVIRELAEMGYITSFCTAGYRCGRTGNHIMDLLRSGKEGCFCKLNAILTFREWLDD
                     FASPETAKAGERIIQKEIEETRDKVPEKLFRQLMAYYERTSRGEKDLYF"
     gene            4660..>5737
                     /locus_tag="PLY56_05310"
     CDS             4660..>5737
                     /locus_tag="PLY56_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ20932.1"
                     /translation="MEYLFARRLEGMRRSTIREILKVAEQPDVISFAGGLPAPELFPV
                     DAVRAACDRVLIREGSWALQYGPSEGYTPLREYLVEAMAQRGVACRVENVQVTSGSQQ
                     ALDLLGRCFLNPGDVVLTENPTYLAAIQAFQGSEARFVPVPTDDNGLIPEALPELVAK
                     HQPRFLYTIPNFQNPTGITMPAERRRALYETAAKLSLVIVEDDPYGLLRYTGEHVPPI
                     KALDTEGIVIYASTFSKTIAPGLRVGWVVADRQILDKLLILKQAADLHTSSLDQRVAC
                     EFMTSCDTTAHLAQIRASYGTRYQAMDAALRALLPERYQWTHPEGGMFLWVTCPEGVD
                     TWPLMQRAVEHKVLFVPGCDFFPEG"
BASE COUNT          961 a         1857 c         1819 g         1100 t
ORIGIN      
        1 agtgcgcgtg atgttgacga agataccgac gtactgccgc tcttcgcgca acgcgtactg
       61 gatctggtgg cagaccaggt tatgccgggc atgtttcacg gtggtctcga tcagatccgt
      121 ctttccggtg gcgagctgct cgaacggctc ggcatccatc aggtaggaga ttggccgacc
      181 acatactgcc tcggagcaca tgaagaagcg tcggaacgcc gggttcatgc tcaagatccg
      241 caggtgctca tccaggatga cgatgccgtt ggggctcgtc tcgatgatgc ggtcggtgcg
      301 ctgctcggct aggcggcgca tgtaggggat gcacattccc ggctctgcca tgccacggac
      361 gaccgcaatc gccttatcgc gacatgtcgc gtagccgcac gcgccgcaat tgagctggtt
      421 ctcggggttc gccttacctg tctgctccag gacggcgcgg atctgctctt ccgacacccc
      481 ttcggcatcc aggaccggct gagccgcaaa gcgggtgtcg agaccgtggg tgtcggtata
      541 gggttgaggg gtacgtccgc gtgcggacgt cgcgtagccc aggagcacct ggcgacgggc
      601 gtaagcgttg gcccgggtgg gcatcgccgg accgttgatg catccctggt gacagaaaag
      661 cggctcgatg atcatcggca gcccgctggg cttgagcacc tccagcgact cgcgaagctc
      721 gtcaaagccg ctcgcggaga tggtctgcgg cgcgagaaca tcggtctcca tgtgggcggt
      781 acgcaagatc ccgccgacga gcgggaagta gcgcgattca ccctctggct cctcgtcgaa
      841 atggctctct tcacactgcg ccagctgaat gccctctcgc tcgaaccact cttccagctc
      901 cgtgaaggtg agtacacaat cgaccacgcc ggcgttctcc ggtcgttctg cctccgcctt
      961 tttggcgacg catggcccga taaagacgac tcgcgaacca tggccgaggc gttccttgat
     1021 gtgccgggcg tgtgccagca tcggcgagac gaccgggatc atcagatcca ggagatccgg
     1081 acggtagcgc tcgatatagt tcaccaccac gggacacgcg gtggtgatgt gcgaccggtt
     1141 ggggcgcgca tccacgatct cgcgggttcg cagcgccgta tggtaggcgc cgatggctgt
     1201 ttcgcccacg taggcaaacc cgagccggcg cagcgccgag gggaggcact ttcgctccca
     1261 ttcggtgaag gcagacgcga acgaaggcgc gatgctcgca cccactggaa cccccgaggc
     1321 gatgagacgc gctgcctgat cgacgtcgtt tcggaatgcc ttggcgccct gtgggcactc
     1381 ccggatgcac gtgccgcagg agatgcaccg ctctgccacc acgtaggcct ggccatcatc
     1441 catcccaatg gccttgacag ggcagacgcg cagacaccgg tagcaatccc ggcagtgtgc
     1501 tttgttcgtg taaacgacct gtcccccctg gctgctcatg attctcctcg tcgcgtcacc
     1561 ggcgggcgtt ctattcgccc ctgcccggtg gttggcccag ccgggcaggg gcccctccca
     1621 cgcaccctcc cacaggtatg tcaatcccag cctaagcggc tggccgggca tcccgtagcg
     1681 tctcgaaagg gcgcagcacc cgatcgagat tgccaagcgc ggcggcgatg gccatgccgt
     1741 agttggtgac cggcacgccc tgctcgcggc agcgtaagat gcgcgagagc atcccccggc
     1801 ggttcaacac gcatccgccg caatggatca ccacgtcata gtctgccagg ccctcaggaa
     1861 actccttgcc gctcgccacg tcgatcgtca ggccggcccc gacgcggttg cgcagtaacc
     1921 gggggatctt cacccggccg atatcctccc cgaaggggtg gtgtgtgcac gcttcggcga
     1981 tgagcacctt gctctccggc gtcagttcca tcaacgcgcg cgcgccctgc gcgagggcgt
     2041 ccaggtcgcc cttgaaacgc gcgaacagga tcgagaagct ggtgagcggg atctcgggcg
     2101 gtgtcgccgg catcactgta gcgaacgcct gagaatcggt gatcaccagt cgaggcggct
     2161 cgcgcagctg atcgagtgat tccgccacct gatccggacc gatcacgtag gagcgcaccc
     2221 cgtactcgag gcactcgcgc agcacctgct gcaccggcag gatcagccgg cccttgggcg
     2281 cctcctcatc cagcggcacc accaggatca cggtgtcgcc acgctggaga aggtcgcaca
     2341 cgatcggccg gctgttgatc cactcctctg gagccagctg gagcagcttc tccttgatca
     2401 gcgccgccgt ctcctgcggc gcgtccttcg ccgagatctc ggcgaagggg atcttggcgc
     2461 gttccaggtg ctcgcggaga gccggcgcgg tagggccctg atccctcttg ttcacgacga
     2521 ccatcacggg cacctggcgc tcttgcaggc gatgcaacag gccctcttcc gcgtcgcccc
     2581 acccgtccgg ctccgtcacc aggatcgcca gatcggtgcg gtcgatcgcc ttcatcgtgc
     2641 gcgccacacg ttccgccccg agcgtggccc ggtcgtcaat ccctccggta tcgatgaaaa
     2701 ggaccggccc tagcggcagg agttccatcg ccttctcgac cggatcggtg gtggtgcccg
     2761 gctcggcgga gacgatcgcc acgttctgct gcgtgatggc attcagcagc gacgacttgc
     2821 ccacgttggt gcgcccgaag aggccgatgt gcaggcgcat tccctttggt gcctgattgt
     2881 tcatcctcgt ctcccgcaat gcggctctcg ccggcggcct ccgcttcccc tagaaataga
     2941 gatccttctc gccgcggctg gttcgctcgt agtaggccat cagctgccgg aagagcttct
     3001 cgggcacctt atcacgggtt tcctcgatct ctttctggat gatgcgctcg cctgccttgg
     3061 cggtctccgg agacgcgaag tcatccagcc attctcggaa ggtgaggatg gcgttgagct
     3121 tgcagaagca gccctccttg ccggagcgga ggagatccat gatgtggttt cctgtgcgcc
     3181 cgcagcggta gcctgccgtg cagaaggagg tgatgtagcc catctcggcc agctcgcgga
     3241 tcacgctatc gagcgaggtg gtatcgccca gggtgaactg ctggcggtcg gcctcctgct
     3301 gttcgtactc atcttgatag gcgcccactc cgatgcgcgt cgacgcgtcg cgctgggtgc
     3361 acatcgggat gcattcgcgg atcacgtccg ggcgctcgcg cacggtgatg atcatccccg
     3421 tgtagggcac cgagagccgg atcacgcaga ccaggcgctt gaactcctta tcggagacgc
     3481 gataccgcgt ttcctggaca aacggcgtgt cgagcgcggg ggtaagccgc gggaaggaga
     3541 tagtatgcgg gccgatcccg aagcgcgcct ccagctcgtg cgcgtgagcc accaggccca
     3601 ttacctcgaa gcgccagtcg tagagcccga agagcgcacc gatcccgacg tcatccacgc
     3661 cggcttccat agcgcggtgc atgcaggtga tccgccaggc gtagtccgac ttcaccgtgc
     3721 cgttcgggtg gagtttcgcg taggtgtcgt ggtggtaggt ctcctggaac acctggaagg
     3781 tgcccaggcc cacctctttc aggaggcgca gatcgtcgat cgacatcggc gccgcgttga
     3841 tgttgagccg gcggatcccg gtggaggaac cggtgcgcgt gggcacttcg accgcgtaga
     3901 cggtgcgcat cgcttccgcg atgtagcgcg cgtcggtgcg cgggtgctcg ccgaaaacaa
     3961 gaatcaggcg cttgtgaccg atctggccgg ccagcacctc ggcctcgcgg gcgatctcct
     4021 ccatggtgag gcaacgccgc tttatcccac tgttgcccgc gcggaagccg cagtagaggc
     4081 agttgttcac gcagagatcg ctgcagtaaa tcggcgcgaa ggtgaccacc cggttgtcat
     4141 ataccttctt cttgacttgc gccgcgacgt cgtacatctc gtcccacagc gcctcgtcgg
     4201 aaacgctcaa aagcgttgcc gtctcctcgg tcgtgagagt ctcaatcgcg agcgacttct
     4261 ggaggatatc gcggacgcgc accgggtccg gcttggcgga ggcgagctgc gcctccagcg
     4321 ccgcgtcgtc aatgaaatcg cgcccatcct gcaaatagcg ggtcacctcg tcccgcttga
     4381 tcacctggct ggcccaagtc ggcttcggtt ccactagctg tgcactcatt gcctgctcgc
     4441 tctcgtactc gttccggctt ccgccggtgt ccttcgccgc tctcctgctg gcggctatgg
     4501 atagatcctt atcgataaaa ctgtatccat tgaccattat tgcacacccc cgcacgcttg
     4561 tcaagcatcg ttctgggaat cccgaaagga ggattttcgc gttgacagat ggatcgcatc
     4621 gtgctacgat tcagatatcc tcggtttgga agggctacaa tggaatacct gttcgcgcgc
     4681 cgcctggagg gaatgcggcg ctcaaccatc cgcgagatct tgaaggtggc ggaacagccc
     4741 gatgtgatct cctttgccgg cgggctcccg gctcctgaac tctttcctgt agacgctgtt
     4801 cgcgcggcgt gcgaccgtgt tctaatccgc gaagggtcgt gggcgctgca gtacggcccg
     4861 agcgagggct acacgccctt gcgcgagtat ttggttgagg cgatggcgca gcggggcgtc
     4921 gcctgccggg tggagaacgt gcaggtgacc agtggttctc agcaggcgct ggacttgctg
     4981 gggcggtgtt ttctcaatcc gggcgatgtg gtgctgacgg agaaccccac ctatctggcg
     5041 gcgatccagg cgttccaggg ctcggaggca cgcttcgtgc ccgtgcctac ggatgacaac
     5101 ggcctgatcc ccgaagcgct tccggagctc gtcgccaagc accagccgcg gttcctctac
     5161 acgatcccca acttccagaa tccgaccgga atcaccatgc ctgcggagcg ccggcgtgcc
     5221 ctctatgaga cggcggccaa gctgtcgctc gtcatcgtgg aagatgaccc ctacggcctg
     5281 ctgcgctata ccggagagca cgttccgccg atcaaggcgc tcgacaccga gggcatcgtc
     5341 atctacgcga gcaccttctc gaagacgatt gctccggggc tgcgcgtcgg ttgggttgtg
     5401 gcggataggc agatcctcga caagctcctc atcctgaagc aggcggcgga tctgcacacc
     5461 tccagtctgg accagcgggt cgcctgcgag ttcatgacaa gttgcgatac cacggcgcac
     5521 ctggcgcaga ttcgcgcctc ctacggaacg cgctaccagg caatggacgc ggcgctgcgc
     5581 gccttgctcc ccgagcgcta ccagtggacc catccggaag gcggcatgtt cctctgggtg
     5641 acctgccccg agggggtaga tacctggccg ctgatgcagc gtgcggtgga gcacaaggtg
     5701 ctcttcgtgc ccgggtgcga cttcttcccg gaaggtg
//
LOCUS       DAOSCV010000159         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8951_length_5707_cov_2.999646, whole genome shotgun sequence.
ACCESSION   DAOSCV010000159 DAOSCV010000000
VERSION     DAOSCV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8951_length_5707_cov_2.999646"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLY56_05315"
     CDS             <1..773
                     /locus_tag="PLY56_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ20933.1"
                     /translation="LKIIARWGVGIETIDLAAATRRGIVVTNTPGATTESVADYSFGL
                     ILDLARRLTEMHNALRAGRWERLWGVDVWRKTLGIIGFGHIGQGMARRARGFEMTTLA
                     YDPYAPPEAGAELGAERTSLDDLLERSDFVTLHASLTPETRGMIGEAELRRMKPTAFL
                     VNCGRGALVKTDALVRALREGWIAGAAVDVYDVEPAPHDHPLLSLPNCLTLPHSASLT
                     HECARIINRMNADDLLAVARGDRPRFVCNPEVYEAGKA"
     gene            776..1489
                     /locus_tag="PLY56_05320"
     CDS             776..1489
                     /locus_tag="PLY56_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ20934.1"
                     /translation="MGKRWSAGALEDDTGAPILRVPSLQALALERIRDAILEGRLPPG
                     TPLRINALAAELGMSPIPVREALQVLATEGLAVHLPHRGMTVSPLRAEDVAQTYEVRA
                     VLEGLAARHAAGRLAPAVMANLRALLAEMETTHECQDHEALLRLDRAFHACICEAYPN
                     RWASEFLRQIWNHAYRVRRTYPRSKARLRVVLGEHQAILAALDAGDGERAENLVRRHL
                     EGARDDLLERLQSARSAAA"
     gene            1743..4268
                     /locus_tag="PLY56_05325"
     CDS             1743..4268
                     /locus_tag="PLY56_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOJ20935.1"
                     /translation="MSVCRIELLGALRVHCAEREITRFRTQRTGALLGYLALSYPRAC
                     IRDHLVEAIWPDAEPESGRHNLRTALSSLRRQLEPPGVEPGSVLVCGRNLVALNPDSV
                     ATDVAHFESALRLALRVGSASEKVLAWVEAIETYRGELLPGLSDLWVFGERQRLAGRY
                     FETVERVVSYFERLRDYTSALEYAYRAVRIDPLRDESHRAVIRIQIAAGHPDDALAYY
                     QEWERVSTRELGCPPAEGTRALVEGLEEAARRTRVSLAGREPVGIAREHDGLPFPGSD
                     ASEASPAVHPDRGEPEPLPNDTVTFLLADMAACEVEGGMRPEPNEALAECRLIARAEL
                     EQHGGCELDGEGIVAVFTSETSALRCAIALQQAARARLWPLRVALHTGDATRVDGPDP
                     HGTPAWRQVCQVLQAGGRVLRAMHAGQIVCTEETGILLRRSQYPPVELAELGAYYLAP
                     GGSPERLFLVRDGAMPGDALPPLRAEAVVTPELPAPLTRFYGRREEMCRLRQLLTAPD
                     VRLVTLTGSPGIGKTRLALEVARDLLEAFRGAVWYVPLAEMHDAGGLLDALTDALGLD
                     SSFGGDALSRIVSHLAGRRALLVLDNLEQMGSAVGPVIAALLTRVPQATCLVTSRCAL
                     AIPGEHQVVVHPLPVPAGEEEPQDLLHYASVELFLDRAQAVRPDCQVTSASAPTIAAL
                     CARLEGIPLALELVAARAGVLSLPQMLARLDGLLDWQASPGACATERHRSLRAAIDWS
                     YGLLSPELRRALERLSVVRDGWSLRTTQSVCGQGESLSEKECLDLIWELARHSLLIAE
                     ECCGEMRFRMLGVIRQYASERLWMRGAAEKTRRRYAECFLRLA"
     gene            complement(4320..>5707)
                     /locus_tag="PLY56_05330"
     CDS             complement(4320..>5707)
                     /locus_tag="PLY56_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOJ20936.1"
                     /translation="IKNAGLNFPAGRRITINLAPGDTRKEGPSFDLPIAIGILAATGQ
                     VKSEGLEEWAFIGEMALDGTVRPVNGVLPMAIGCRDAKKKRLIVPEANAREAAVVGEV
                     QVYPATDLAQVVRLLENPDAVSPFVVEQPDGHLIQPLYEHDFAEVKGQEHVKRALEVA
                     AAGGHNVIMVGPPGSGKTMLARRLPTILPAMSVEEALEVTKIYSVSGLLSHNAGLVST
                     RPFRSPHHTVSTVGLVGGGSVPRPGEVSLAHRGVLFLDELPEFRRDALEVLRQPLEDG
                     QVTISRAAAAYTYPASFMLVSAMNPCPCGFYQDPTRQCTCSPGLIQRYLQRISGPLLD
                     RIDIHIEVPRLKLDEFTATAQVGESSAQIRARVEAARQRQTERFRKTGLYCNAQMQSR
                     HIRKWCELGEDGRELLKAAIQQLGLSARAYDRILKLSRTIADLDASDEIRVAHIAEAI
                     QYRSLDRKFWA"
BASE COUNT          841 a         1837 c         2074 g          955 t
ORIGIN      
        1 ggctgaagat cattgcgcgc tggggcgtgg ggatcgagac gattgacctg gcggcggcga
       61 cgcgccgagg catcgtcgtc accaatacgc ccggtgccac cacggagagc gtggccgact
      121 actccttcgg attgatcctg gacctggcgc gccgcctcac tgagatgcac aacgccctgc
      181 gcgccgggcg ctgggagcgg ctttggggcg tcgacgtgtg gcgcaagacg ctcggcatca
      241 tcggcttcgg gcacatcggc caggggatgg cgaggcgcgc gcgcggcttc gagatgacca
      301 cgctcgccta cgacccctac gccccgccgg aggccggcgc ggagctggga gccgagagga
      361 catcgctgga tgacctgctg gagcgctccg acttcgtgac cctccacgcc agcctcaccc
      421 cggagacgcg cgggatgatc ggcgaggcgg agctgcgccg gatgaagccc accgcttttc
      481 tggtgaactg cgggcgcggc gcgctggtga agaccgatgc cctggtgcgg gcgctgcgcg
      541 agggctggat cgcgggcgcg gcggtcgatg tgtacgacgt ggagccggca ccgcacgacc
      601 acccactcct ctcgttgccg aactgcctga cgctgccgca cagcgccagt ttgacgcatg
      661 agtgcgcccg tatcatcaat cggatgaatg ccgatgacct tctggccgtt gctcgtggcg
      721 accgcccgcg ttttgtctgc aacccagagg tgtatgaagc cggaaaggcg tgagtatggg
      781 aaagcgttgg agtgcggggg cgcttgagga tgacacaggg gcccccatac tccgggtccc
      841 gtcgcttcag gcgctcgccc tggagcgaat ccgcgatgcg atcctggagg ggcggctccc
      901 gccgggaacg ccgttgcgga tcaacgcgct ggcggcggaa ctggggatga gccccatccc
      961 ggtgcgggag gcgttacagg ttcttgccac cgagggtctg gccgtgcacc tgccacaccg
     1021 gggcatgacc gtcagcccgt tgagggcgga ggatgtggcg cagacgtatg aggtgcgggc
     1081 ggtcctggaa ggcctggctg ctcgccatgc ggccgggcgg ttggcgccag cggtgatggc
     1141 gaacctgcgg gcgttgctcg ccgagatgga gaccacgcac gagtgccagg atcatgaggc
     1201 gctcctgcgg ctggaccgtg cctttcacgc ctgcatctgc gaagcctacc ccaaccgctg
     1261 ggccagcgag ttcttgcgcc agatctggaa ccacgcctat cgtgttcgcc gtacctaccc
     1321 gcgctcgaaa gcgcggctcc gcgtggtgct tggcgagcat caggcgatcc tggcggcatt
     1381 ggatgctggc gatggcgagc gagccgagaa cctcgtgcgc cgccacctgg agggcgcccg
     1441 cgatgacctg ctggagcgcc tacagagcgc caggagcgcg gccgcgtagc cggccggcga
     1501 ttctcctctc cgggaaggca gcggtcatgg catggccggg gggagagcca gctagcgagg
     1561 cgcgcgtgcc cggaagagac accattcgcc gtgcctcagc agagacactt ctatggagat
     1621 caggcgcgat ggacggccca tctctcattc gccgaaggag tgggtcaccg tgacgtgcgc
     1681 actcgcgcgc gcctggatcg aggcatgggt gcctcgcaca accctggact gggagcccaa
     1741 ccgtgtctgt gtgcaggatc gagttgctgg gcgcgttgcg cgtccactgc gccgaacggg
     1801 agatcacccg atttcgcact cagcgaacag gcgcgctgct tggctacctt gccctgagtt
     1861 accctcgcgc atgtattcga gaccacctgg tcgaagcgat ctggccggac gccgagcctg
     1921 aatcgggccg gcataacctc cggaccgcgc tctcttcgct gcgccgccag ttggagccac
     1981 cgggggtgga gcccggcagc gttctggtct gcgggcgaaa cctggtagcc ctgaaccccg
     2041 attcggtagc gaccgatgtg gctcacttcg agagtgcgtt gcgtctggca ctgagggtgg
     2101 gcagcgcatc cgagaaggtg ctggcctggg tcgaggcgat cgagacctac cgcggcgagc
     2161 tgcttccggg gctctcggat ctgtgggtct ttggcgagcg acagcgcttg gcggggcgct
     2221 acttcgagac ggtcgagcgg gtggtgtcct acttcgagcg gctgcgtgac tacaccagcg
     2281 cgttggagta tgcctatcga gcggtgcgca tcgacccgct gcgcgatgag tcgcatcgcg
     2341 ctgtgatccg gatccagatc gcggcgggac atccggacga tgcgctggcg tattaccagg
     2401 agtgggagcg cgtttccact cgcgagctag gatgccctcc cgcggagggg acgcgcgctc
     2461 tggtggaggg gctcgaagag gcggcccggc gcacgcgcgt ctctctggcc ggccgggagc
     2521 ctgtcggcat tgctcgcgag cacgacggcc tgcctttccc agggagcgac gcctcggagg
     2581 cctcgcccgc ggtgcatccc gaccgaggcg agccagaacc gctccccaac gatacggtca
     2641 ctttcctcct ggccgacatg gcggcgtgcg aggtggaggg tgggatgagg ccggagccca
     2701 atgaggcgct cgcggagtgc cggctgatcg cgcgcgcaga gctggagcag catgggggat
     2761 gcgagcttga cggcgaggga atcgtggcgg tcttcaccag cgagacgagc gcgctccgct
     2821 gcgccattgc gctccaacag gcggccaggg cgcgactctg gccgcttcgg gtggctctcc
     2881 acacagggga tgccacccgc gtggatggcc cggacccaca cggtaccccg gcctggcgcc
     2941 aggtgtgcca ggtgctacag gcgggaggac gcgtcctccg cgccatgcac gcggggcaga
     3001 tcgtgtgcac ggaggagacc ggaatcctcc tccggcgctc gcagtatccc cctgttgagc
     3061 tggcagagct gggggcgtac tatctcgctc ccggcggttc cccagagcgg ctgttcctgg
     3121 tgcgggatgg ggcaatgcct ggcgacgcgc tcccgccatt gcgcgccgag gcggtcgtga
     3181 cgccggagct gcctgcgccg ctcacccgct tctatggccg gcgcgaggag atgtgccggt
     3241 tgcgccagct cctcactgct cccgatgtgc gcctggtgac gctgaccggc tcccctggaa
     3301 tcggtaagac gcggcttgcc ctggaggtcg cgcgtgatct cctggaggcg tttcggggcg
     3361 ccgtgtggta tgtgccactg gcggagatgc acgacgcggg aggtctcctc gacgcgctga
     3421 cagatgccct ggggttggat agttcattcg ggggcgacgc gctgagccgg attgtctccc
     3481 atctcgccgg ccggcgggcg ctgttggtgc tggataacct ggaacagatg gggagcgcgg
     3541 tggggccggt gatcgcggcg ctccttaccc gcgtgccgca ggcgacgtgc ctggtcacgt
     3601 ctcgctgcgc gctggcgata cccggggagc accaggtcgt ggtccatccg ctcccggtgc
     3661 cagccgggga agaggagccg caggatctgc ttcactatgc cagtgtcgag ctcttcctgg
     3721 accgggctca ggccgtgcgc cccgactgcc aggttacctc ggccagtgcc ccgaccatcg
     3781 ccgcgttgtg cgcacggttg gagggcatcc cactggcgct ggagttagtg gccgcccggg
     3841 ccggagtgct gtcgctaccc cagatgctcg cgcgcctgga tgggctgctg gactggcagg
     3901 ccagccccgg cgcgtgcgcg acagaacgcc accgctcgct gcgcgcggcg atcgattgga
     3961 gctacgggct tctctctccc gagttgcggc gcgcgctgga gcggctctcg gttgtgcgcg
     4021 acgggtggag cctgagaacc acccaaagcg tctgcggcca gggcgagtcg ctttcggaga
     4081 aggagtgcct ggatctgatt tgggagctgg cgcgccactc gctgctcatc gctgaggaat
     4141 gctgcggcga gatgcgcttc cggatgctcg gggtgatccg gcagtatgcc agcgagcgcc
     4201 tctggatgcg cggggccgcc gagaagacgc gccggcgcta cgcggagtgc ttcctccgcc
     4261 tggcgtagga gaccggccct gagagatatc ccccggtcag gctggtggct cgcgacggtt
     4321 tacgcccaga acttgcggtc gaggctgcgg tactggatgg cttcggcgat gtgggccacg
     4381 cggatctcgt cgctcgcatc caggtcggcg atggtgcgcg agagcttcag aatccggtcg
     4441 taggcgcggg cggagaggcc taactgctgg atggcggcct tcagcagctc acgcccgtct
     4501 tcgcccagct cgcaccactt gcggatgtgc cggctttgca tctgcgcgtt gcagtagagg
     4561 cccgtcttcc gaaatcgctc agtctggcgt tggcgcgccg cttcgacgcg cgcgcgaatc
     4621 tgtgcggatg actcgccgac ctgggcggta gcggtgaact catccagctt caggcggggc
     4681 acctcgatgt ggatgtcgat ccggtcgagc agggggccag agatccgctg gaggtagcgc
     4741 tggatgagcc cgggcgagca ggtacactgg cgcgtgggat cctgatagaa gccgcagggg
     4801 cacgggttca tcgcgctcac gagcatgaac gacgccgggt aggtgtaagc ggcggccgcg
     4861 cgcgagatcg tcacctggcc atcctccagg ggctggcgca ggacctccag ggcgtcgcgc
     4921 cggaactcgg gaagctcgtc gaggaagagg acccctcggt gggcaaggct cacttctccg
     4981 gggcggggta cgctgccgcc tcccacgagg ccaacggtgc tgaccgtgtg gtgcgggctg
     5041 cggaagggac gcgtggagac cagcccggcg ttgtgcgaga gaagcccgga aacggagtag
     5101 atcttcgtga cctccagcgc ctcttcaacg ctcatcgcgg gcaggatcgt cgggagccgg
     5161 cgcgccagca tcgtcttgcc cgacccgggc gggccgacca tgatcacgtt gtgcccgccg
     5221 gccgcggcca cctccagcgc acgcttcacg tgctcttggc ctttcacctc ggcgaagtcg
     5281 tgctcgtaga gcggctgtat caggtggcca tccggttgct ccaccacgaa gggagagacg
     5341 gcgtccgggt tctccagcag gcgcaccacc tgcgccaggt cggtcgccgg gtagacctgg
     5401 acctcgccga ccacggcggc ctcgcgcgcg ttcgcttccg gcacgataag ccgcttcttc
     5461 ttcgcgtcgc ggcacccgat ggccatgggg aggacgccgt tgacggggcg cacggtgcca
     5521 tcgagcgcca tctcgccgat gaacgcccac tcctccaggc cctcgctctt cacctgcccg
     5581 gtggcggcca ggatgccaat cgcgatcggg aggtcgaagg atgggccttc cttgcgcgtg
     5641 tcgcccggcg ccaggttgat ggtgatcctc cgcccggcag ggaagttgag ccccgcgttc
     5701 ttgatgg
//
LOCUS       DAOSCV010000160         5706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8952_length_5706_cov_3.759158, whole genome shotgun sequence.
ACCESSION   DAOSCV010000160 DAOSCV010000000
VERSION     DAOSCV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8952_length_5706_cov_3.759158"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLY56_05335"
     CDS             complement(<1..609)
                     /locus_tag="PLY56_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20937.1"
                     /translation="MKTLVHVTHEAVVKVGGIGAVLHGLFTSRSYAREFGRSLLIGPL
                     WGHESPETVLSSTGTVLYSSIHGIDVGGYGAQLRPVEEHLGTRFVYGRRRFTDPLTGV
                     SLDPEVLLVDVRRLRPEPLATFKYNLFRRFGMDSYRWDASWEYEQYVRLGVAGYAALP
                     ALEIGRPGDQTCILAHEFMGLPLAYAAIMERNPGFRTFLYAHE"
     gene            946..1021
                     /locus_tag="PLY56_05340"
     tRNA            946..1021
                     /locus_tag="PLY56_05340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:979..981,aa:Lys,seq:ttt)
     gene            1416..5267
                     /locus_tag="PLY56_05345"
     CDS             1416..5267
                     /locus_tag="PLY56_05345"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOJ20938.1"
                     /translation="MRIGLMATLTLLLAIPAAVAFPLCDPAAPWVTAGAPGVAPTLDG
                     VIDAREWAGSVPLTGFGGVNTQELSSIQPTVRVARDAAHLYLAVHVPLPGGRAPVAKA
                     RQHDGAVWEDDSVEVFLDPGHGHQEYFQFTGNAIGTRLDARGKDAAWNGEWQFQARAG
                     KGSWSAELAIPFATLQMRAPGENAVWGFNVCASIRGVGSISWAPVLRSFHEPARFGHL
                     RFSSAGAAGIETLAGLLQGGGEIKGVARGTGVSLRWQLSRDGAQAIEKRLSGSGEFAF
                     PVELPRDGRFPAAGRYTLEIVAERAGDKLPLMRLAADHTVSPPLQLRARAFVYAGRLE
                     TEAAVEAAGLNPADLACRFALAPEGGKPSVTREAALENGRTARATFQKAEVPEGKITV
                     SVTLLEKGMPIRVASKVLDRPLSPPWLGDRTGITDEVPAPWTPLKVRGKEARAGARGS
                     AVSVRPWGRDYRFEGGLFPAQVTAKGASVLAGPITLRAARAGQALPWREAWTKVSKNA
                     PSAVDLAGGAVTTGARLSGTARVEFDGMLRVDLELSPEGGAAPDMLVLEVPLRAEHAR
                     YLYHFPGQWGSVENSGALPAEGWHHAFKPFVWLGDEERGFSWFCESEENWSPADPERA
                     ITIDREGDRVVLRLHLVEGAPVQKPLKYTFGFQATPVKQPEKDAWDYRIHHGGSYTLH
                     KDLYTRQGRVAYPAAGLIRGEQGTFEAWVQPLFDSDPSLPAEKKRGWGNRGIFSVRLP
                     DDTNFGIYWNELVQGPVVWVRENGNVTFYAGAPIACKSGEWHHLAFTWGDAIRCYYDG
                     ECVFERPRRGLTATPLELENTEIVLGERLPHFVIDEVRILDVARAPVVPAGPLEADAH
                     TLFLERFERLGEANTFQPEKGPAGKLHGGATLVEGRFGKGLAMSTRGMAGYTKLDRLA
                     EMGVRTVCFHEHWTPWQSHPYTTPENEPKLAELVKAIHAKKMQLLLYMGRKIADIAPE
                     YELYSEDVLMMPRGRRYTRQPPQTDWGICWRSHWKEFALKGLAETMDRFGSDGWYLDG
                     SEWPCPCTNRDHGCGYVKPDGTIGPTYDIFATREFMRRLYVLTRQRRPDGQLNIHNST
                     VMVIPTLAWGTSTWNGEQLGSIDRGPEFLKLMPLDAFRTEMMGHQFGVPSEFLCYERP
                     WTTHEALSFTLLHDVLVRPNGYDDRLEEVASLWRAMDAFGREKARWLPYWKNADLVRP
                     EHEAVKVSLYTRGKEGALLVVSNLSAEARTAKVELNLKRLGLSAGAKAVDARTNEPVT
                     LENGRLAVELGAFDYAIVRVR"
     gene            5433..5508
                     /locus_tag="PLY56_05350"
     tRNA            5433..5508
                     /locus_tag="PLY56_05350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5466..5468,aa:Met,seq:cat)
BASE COUNT          937 a         1866 c         1954 g          949 t
ORIGIN      
        1 ttcatgcgcg tagagaaacg ttcgaaagcc cgggttgcgc tccatgatgg cggcgtaggc
       61 cagtggcagg cccatgaact cgtgggcgag gatgcacgtt tggtcgcccg gccttccgat
      121 ctccagcgca gggagcgccg catagccggc cacgcccagg cgcacgtact gctcatactc
      181 ccaggatgca tcccagcggt aagagtccat cccgaagcgg cgaaagaggt tgtatttgaa
      241 cgtggccagc ggctccgggc gcaggcgacg cacatcgacc aggagcacct ccgggtcgag
      301 tgagaccccg gtgaggggat cggtgaaccg cctgcgaccg tagacgaagc gggtgccaag
      361 gtgttcctcc accggacgga gctgggcgcc gtagccgccc acatcgatgc catgaatcga
      421 ggagtagagc accgtgccgg ttgatgagag aaccgtctcc ggggattcat gcccccagag
      481 cgggccgatc aggagcgaac gcccgaactc gcgcgcatag gagcgggagg tgaagaggcc
      541 atgcagcacc gcgccgatgc ctcccacttt caccaccgct tcatgggtca cgtgtaccag
      601 tgtcttcatc cgctctcctc cctcccaccg tcgttgacat gcgccgggag cgggaaggga
      661 cgcgaacgct ccgcgggccg tgctcctggc accggccctc ccggccatcc ctcttcagct
      721 gcggcttccc gtttgcgcca ctccaacgga ttccccttag gttcctaccc ggtcgcgcat
      781 gtggccaaac ggagctatcc cgacggcttc cctgttcctt cggcgcacgc acctcgaacg
      841 cctgcttttc ggatgggatg gattcactgt gttgccgcga gcagcatttt ctgctataat
      901 agagatgaac gctggcctcc gcgagggagg accgggtgaa gccacgagcc gttagctcag
      961 ccggcagagc atctcccttt taaggagagg gtcgttggtt cgaatccaac acggctcacc
     1021 agaacttcat atcgaattgt tcccatccac aagctttcct gccctctcgg ctcccaatgc
     1081 ccgtgccgca ggccggctct ctcaaggaga gtgtctgcac agggcgtttg gacgccaaag
     1141 agacggctta ctcgcttcgc aaggttgatg ccggaccgtt cttcctttgc cgcgctcgcg
     1201 tgttgcgtag ccccagatgg agcgtccgct ccctttgctc gtggtctcat tgcccgtgca
     1261 atcagcgatt gactcctcag tggctggcgt acccttttct cgcgggcctg ccacaaacgc
     1321 cagccgacac cccttctggc aaggagcggc gcgcccctag agcgaaggat gtagcatccc
     1381 ggcggctgca ccctggccgg cgaaggaggc acccgatgcg tattggtctc atggccacac
     1441 tcactcttct gctcgccatt ccggcggcgg tcgcgtttcc cctgtgcgat ccggcggcgc
     1501 cgtgggtcac cgccggcgcc cccggagtgg cacccactct cgatggagtc atcgacgccc
     1561 gcgagtgggc cggctctgtg ccgctcaccg ggtttggggg cgtaaacacg caggaactct
     1621 cttccattca gcccacggtg cgcgtggcgc gcgacgcggc gcacctctac ctggcggtcc
     1681 acgtgccgct cccgggaggc cgggcgccgg tcgccaaggc ccggcaacac gatggcgccg
     1741 tgtgggaaga cgactcggtg gaggtcttcc tcgacccggg ccacggccac caggaatact
     1801 tccagttcac gggcaacgcc atcggcacgc gcctggacgc gcgcggcaag gatgccgcgt
     1861 ggaatggcga gtggcagttc caggcgcgcg cgggcaaggg cagctggagc gccgagctcg
     1921 cgattccgtt tgccacgctc cagatgcgcg cgcctgggga gaacgcggtg tggggcttca
     1981 acgtctgcgc gagcatccgc ggcgttggga gcataagctg ggcgccggtg ctccgctcgt
     2041 tccacgagcc agcgcgtttc gggcacctgc gcttctcgag tgcgggcgcg gcgggcatcg
     2101 aaacgctggc cgggctgctc cagggcggcg gcgagatcaa gggcgtggcg cgcggcacgg
     2161 gagtgagcct gcgttggcag ctctcgcgcg atggcgcgca ggccattgag aagcggctct
     2221 cgggttcggg agagtttgcc ttcccggtgg agctgccgcg agatggccgg ttcccggcgg
     2281 cggggcgcta cacgctcgag atcgtcgccg agcgcgcggg tgacaagctg ccgctgatgc
     2341 gcctggcggc cgatcacacc gtttccccgc cgctccaact gcgcgctcgc gccttcgtct
     2401 acgcgggccg gctggagacg gaagcggcgg tcgaggcagc cggactcaac ccggcggacc
     2461 tggcgtgccg gtttgccctc gccccagaag gcgggaagcc ctcagtaacc cgcgaggcgg
     2521 ccttggagaa cgggcgcacc gcgcgcgcta ccttccagaa ggcggaagtg cccgagggga
     2581 agatcaccgt ctcggtcacc ctcctggaga agggcatgcc catccgcgtc gccagcaagg
     2641 tgctcgaccg gccgctctca ccgccctggc tgggtgaccg gaccggcatc accgacgaag
     2701 tgccagcgcc gtggacgccg ctcaaggtgc gcggcaagga ggcacgcgca ggcgcccgcg
     2761 gttcggccgt ctcggtacgc ccttggggcc gggattaccg atttgagggg ggcctcttcc
     2821 ccgcccaggt gacggcgaaa ggcgcctcgg tcttggccgg accgatcacg ctgcgcgcgg
     2881 cgcgcgcggg ccaggcgctg ccgtggcgcg aggcgtggac gaaagtctcg aagaacgctc
     2941 cgtccgccgt cgacctcgcc ggtggcgcgg tgaccacggg cgcgcgcctc tcggggaccg
     3001 cgcgcgtgga gttcgacggg atgctccggg ttgacctgga gctctcgccg gaaggcgggg
     3061 ccgcgccgga tatgctggtc ctggaagttc ccctgcgcgc ggagcacgcg cgctatctct
     3121 atcacttccc ggggcagtgg ggctcggtgg agaactcggg cgcgctcccg gccgaggggt
     3181 ggcaccacgc gttcaagccg ttcgtgtggc taggcgatga ggagcgaggc ttctcgtggt
     3241 tctgcgagtc ggaggagaac tggtcgcccg ccgatccgga gcgtgcgatc acgattgacc
     3301 gggagggcga ccgggtggtg ctgcgcctcc atctggtgga gggagcgccg gtccagaagc
     3361 cgctgaaata tacgttcggg ttccaggcga cgccggtgaa gcagccggag aaggatgcct
     3421 gggactaccg catccatcat ggcggaagct acacgctgca caaggatctc tacacgcgcc
     3481 agggccgggt agcttacccc gcagccggac tgatccgggg cgaacagggc accttcgagg
     3541 cgtgggtcca gccgctcttc gactccgatc cctcgcttcc cgcggagaag aagcgggggt
     3601 ggggaaatcg ggggatcttc tcggtccgac tgccggatga caccaacttc ggcatctact
     3661 ggaacgagct cgtccagggc cccgtcgtct gggtgcgcga gaatggcaac gtcaccttct
     3721 acgcgggtgc gccaatcgcg tgcaagagcg gcgagtggca tcatctggcg ttcacctggg
     3781 gcgatgccat ccggtgctac tacgacggcg agtgcgtctt tgagaggccg cgccgggggc
     3841 ttaccgcgac cccgctcgag ctggagaaca cggagatcgt cttgggcgag cgcctccccc
     3901 acttcgtcat cgacgaggtg cggatcctcg acgtggcgcg cgcgccggtg gtgcccgccg
     3961 ggccgctgga ggccgacgcg cacacgctct tcctggagcg gttcgagcgg ctcggggagg
     4021 ccaacacctt ccaacccgag aagggccccg cagggaagct gcacgggggg gccacgctcg
     4081 tcgagggccg tttcgggaag ggcctggcga tgagcacccg ggggatggcc ggctacacca
     4141 aactcgaccg gctggccgaa atgggcgtgc gtacggtttg cttccacgag cactggacgc
     4201 cgtggcaatc gcatccctac accacgccgg agaacgagcc gaagctcgcc gagctggtga
     4261 aggcgattca cgccaagaag atgcagctcc tcctctatat gggccggaag atcgcggata
     4321 tcgctccgga gtacgaactc tactcggaag atgtcctgat gatgccgcgc ggacgtcgct
     4381 acacgcgcca gccaccgcag accgactggg gcatctgctg gcgaagccat tggaaggagt
     4441 tcgcgctgaa gggcctggcc gagacgatgg accgcttcgg cagcgatggc tggtacctgg
     4501 acggttctga gtggccgtgc ccatgcacca accgggacca cggttgcggc tacgtgaagc
     4561 cggacggtac catcggtccg acgtatgata tcttcgccac gcgcgagttc atgcgccggc
     4621 tctacgtgct gacgcgacag cgccgcccgg atggccagct gaatatccac aactccacgg
     4681 tgatggtgat ccccaccttg gcgtggggca ccagcacgtg gaacggcgag cagctgggca
     4741 gcatcgaccg cggccccgag ttcctgaagc tgatgccgct cgatgccttc cgcacggaga
     4801 tgatgggtca ccagttcggt gtgccctcgg agttcctctg ctacgagcgg ccgtggacga
     4861 cgcacgaggc gctctcgttc accctgctcc acgatgtcct ggtgcgcccc aacggctacg
     4921 acgaccgcct ggaggaggtg gcgtcgctgt ggcgggcgat ggacgcattc gggcgggaga
     4981 aggcgcgatg gcttccctac tggaagaacg ccgacctggt ccgcccggag catgaggcgg
     5041 tgaaggtgag cctttacacc cgtggcaagg agggggcgct gctcgtcgtc tccaatctct
     5101 ccgcggaagc gcggacggcg aaggtggagc tgaacctgaa gcgcctcggc ctgtcggccg
     5161 gcgcgaaggc ggtagatgcc cgaaccaacg agccggttac cctggagaac gggcgtctgg
     5221 cggtggaact gggcgccttc gactacgcga tcgtccgcgt gcgctgattc ggcgcggaaa
     5281 ctcgtcgggc ggggcgccca gatgggcgcc tcgcccgcac gcgtcccctt gctctctgga
     5341 cagagtggcc cccgtcgcac accatcgcga aatcggtggc cgaatttcct cttgacagga
     5401 aagaccaaaa cgagtagaat agaggcggta agggcggagt agctcagtgg ttagagcaca
     5461 cggctcatac ccgtggcgtc gtcagttcga ctctgacctc cgctaccagt tggggcacgg
     5521 attgcgatcc gtgccccgtt tctttttccg cacccggatg ccacgggggt tgggattccc
     5581 gagatcaagg gagggtcgcg cgcacgcgcg gagctcgaag ctccgcgctg cttctctgca
     5641 aagccctttc agggcttctc ctcccagcca ccgccgtttt gagacatctg ggagtatccc
     5701 caatcc
//
LOCUS       DAOSCV010000161         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_8959_length_5704_cov_3.320234, whole genome shotgun sequence.
ACCESSION   DAOSCV010000161 DAOSCV010000000
VERSION     DAOSCV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8959_length_5704_cov_3.320234"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..942
                     /locus_tag="PLY56_05355"
     CDS             268..942
                     /locus_tag="PLY56_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOJ20939.1"
                     /translation="MDGLEALLDRVREAMDAANSARERALTLSRETIRFSANSIRATH
                     REEYPLAQTLLGEARSRVERMRETLADHPEIYWTGYVQDAQKEFAEASLVLALVQGDN
                     PPGPDDLHVEHAAYLNGLGEAAGELRRHFLDLLRRGNVVRAGEVLSRMDDIYDGLVTF
                     DYPDALTSGLRRTTDMVRGVLERSRGDLTAAIRQEQLQERLRRVEEQIARVSGDPPTG
                     PAPSKP"
     gene            952..1581
                     /locus_tag="PLY56_05360"
     CDS             952..1581
                     /locus_tag="PLY56_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20940.1"
                     /translation="MMQQPRTVTREYAIILFCVVGIAAINAWANNRFTIDFNAMYQTV
                     MGAFGRDGIKRGYERMTLYELLDKHASDPQAVQALKGVAVEGQILRERAIDPDDPNFT
                     QAEKVGTTFRLSRRFSETGIEEDAHVISVEVNYPKPEGLEANMWVLVQGPVRTIEEGE
                     NAGAPIIDAVAVTKMRMKPENDILTPEGEPEHLHHDHGGEMPGHEGHGH"
     gene            complement(1682..2755)
                     /locus_tag="PLY56_05365"
     CDS             complement(1682..2755)
                     /locus_tag="PLY56_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ20941.1"
                     /translation="MSETMLAAMVTAPGRVELREVPRPKLGPYDALTRTLACSFCRGT
                     DTHLIHGTLPFGPPMPFILGHEGVGEVIATGERCRKFRVGDRVLRAGAAYPAGMEGAP
                     RIGWGGFAEYGLLTDIEALRADDPSARPTAGSAAMQQPIPLEMDANEATVLITLKETL
                     SALRRARFQPGMDVLILGTGPVGLAFAMCARALGARKILAAGRHVGRLDRMRRFGADE
                     GADLTAGEIGPLVKAATGGRGVDLAIDAVGDRSRIGALLATLARGGSVGIYGVPEGSL
                     AEYHRYAFDAKGAPDWWSLTVINPDEPSAHEEALGLVERGKLDLGRFVTHTFPLERFE
                     DAVAALASPDAVKVVVTFANKGE"
     gene            complement(2872..3684)
                     /locus_tag="PLY56_05370"
     CDS             complement(2872..3684)
                     /locus_tag="PLY56_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ20942.1"
                     /translation="MRLAYQVATPEVHTPDVTAFRGDLREGFAQLARLGYSAVELMVR
                     DPERVPAAYLEALSREYHLPICLVCTGEVYGEDGLSFTDARVAVRAEAGKRMRAAIRL
                     AARLGVDVNVGRLRGRLGGGVPDEVALEWGRTALLGAADLAASLGIRVLLEPIQRRTA
                     DWARTTEEGLALVRALNHPGLGLMLDTGHMHAEGETIPSSLVAAREHLHHVHLCEENR
                     LAPGMGHLDFPAILGALREAGYDGCVSVEAYQHPDSETAMRVSAETLLPLLG"
     gene            complement(3681..5078)
                     /locus_tag="PLY56_05375"
     CDS             complement(3681..5078)
                     /locus_tag="PLY56_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ20943.1"
                     /translation="MPAQRTILAVEDEPVLRQVLVDALSQAGYCVETAEDGVEAVNKV
                     EQQEYAVVILDIGLPRLNGMDVLRFIKKRRPDIPVIIVTVNATMADAIEALKQGAYEY
                     IRKPFDLGELERIVARAMDRRLLIDENRYLHSELKAHYDFDNVIGMTPEVQQAYLMAT
                     QVAESNATVLITGETGTGKEFLARAIHYQSGRAEKPFLKVNCGALPETLLESELFGHE
                     KGAFTHALSRHIGRFERAHGGTLLLDEIGDISLPVQVKLLRVLQEREFERVGGTETIR
                     VDVRVIAATHRDLEAMIREGTFRQDLYYRLNVIPIHLPPLRERAEDIPTLANHFLERY
                     RKETGKQVTGFTYEALELLQSYDWPGNIRELENCVERAVILTRDERIDVGELFLPSRQ
                     GLSTARPAAYSSSLADGERPLRTLKEIEKEAILDTLERLGGNQSRTALVLGIDRKTLR
                     NKMKEYGLLRSGESE"
     gene            5389..>5704
                     /locus_tag="PLY56_05380"
     CDS             5389..>5704
                     /locus_tag="PLY56_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOJ20944.1"
                     /translation="MKKRATLLLAAVALFAVAMAPPASGAVERPDQDLLIVSGRGEAR
                     VTPNIAVVRLGVESRSARAAEAIQDADRRMNQVLQAIRAQNIPERNIQTVIYNVSRQY
                     RPR"
BASE COUNT         1009 a         1918 c         1804 g          973 t
ORIGIN      
        1 ccgcaggggc tttgccggaa agcagcgcgg agcttcgagc tccgcgcgtg cgggcggccc
       61 tctccttatg tcggaatccc aggctgtgcc ttctgtttct tgacaggcgt tcccgcgtat
      121 gctagcatag aaacgcgttc ggaacgcgcg cggtctccct cgggtcatcc ctataggacc
      181 cgcgattgcc gcggacggac cccgtgccgc tcggtggcac tctctattcc tgtgggttgc
      241 gtaccccgta gccagacggt ggtgatcatg gatggactgg aagcactcct ggatcgcgtg
      301 agagaggcga tggacgcggc caactcggcc cgcgagcgcg ccctcactct ctctcgcgag
      361 acgatccgtt tttccgcaaa ctcgatccgc gcgacgcacc gtgaagagta cccgctggcg
      421 caaacgctcc tgggcgaggc gcgaagccgc gtggagcgta tgcgcgagac gctcgccgac
      481 catcctgaga tctactggac tggttacgtg caagacgcgc agaaggagtt cgccgaggca
      541 tcgctggtgc tcgccctggt ccagggcgac aatcccccgg ggccggacga tctccacgtc
      601 gagcacgccg cctacctcaa cgggctcggc gaagcggctg gcgaactgcg ccggcacttc
      661 ttggacctcc tccgccgcgg caacgtcgtc cgcgccgggg aagtgctttc gcgcatggat
      721 gatatttacg acggattggt gacctttgat tacccggacg cgctcacgtc gggcctgcgc
      781 cgtaccaccg atatggtccg tggcgtactg gagcgtagcc ggggcgatct caccgcggcc
      841 atccgccagg agcagcttca ggagcggcta cgccgtgtgg aagagcagat cgcgcgtgta
      901 tcaggtgatc caccaacggg acctgccccg agtaagcctt gaccggaggg gatgatgcag
      961 cagcctcgaa ctgtgactcg cgaatacgcc atcattttgt tctgcgtggt gggtatcgct
     1021 gcaatcaatg cctgggcgaa taacaggttt accatcgatt tcaacgcgat gtatcagacg
     1081 gtgatgggag ccttcggacg cgatggcatc aagcgcggct atgagcggat gacgctctat
     1141 gagctcctcg ataagcacgc gagcgatccg caggcggtgc aggcgctgaa aggcgtggcg
     1201 gttgaaggcc agattctcag ggagagggcc atcgacccgg atgatcccaa cttcacccag
     1261 gcagagaaag tcggcacgac cttccggctc tcgcgccgct tctcagagac aggaatcgaa
     1321 gaggacgccc acgtcatctc cgtcgaggtg aactatccga agcctgaggg ccttgaggcc
     1381 aatatgtggg tgttggttca gggcccggtg cgcaccatcg aagagggaga aaacgcaggc
     1441 gcgcccatca tcgacgcggt cgcggtcact aagatgcgca tgaagccgga gaacgacatc
     1501 ctcacgccgg agggtgagcc cgaacacctt caccacgacc acggtgggga gatgcccggc
     1561 cacgagggcc acggacacta gttcggagcg aagtcgcttt gccgtatgag gcgcgagccc
     1621 acaagcgggc tcgcgcctcg cgcactctgg aaagcaggtg cccggctttg gcgcgcgccg
     1681 atcattcccc cttgtttgcg aacgtgacca ccactttcac cgcgtccggg ctggcgagag
     1741 ccgccactgc atcctcaaac cgctccagcg ggaaggtgtg cgtcacaaac cgccccagat
     1801 ccagcttccc gcgctccacc agaccgagtg cttcctcatg ggcgctcggc tcgtccgggt
     1861 tgatcaccgt cagcgaccac cagtcgggcg cgcccttcgc atcgaaggcg tagcggtgat
     1921 actccgccag cgagccctcc ggcacgccat agatccccac gctccccccg cgcgcgagcg
     1981 tcgccaggag cgccccgatg cggctccgat cgcccacggc atcgatcgcc aggtcaactc
     2041 cccggccccc ggtggccgcc ttcacgagcg gcccgatctc ccccgcggtg agatccgcac
     2101 cttcatcggc gccgaagcgg cgcatacgat ccaaccggcc cacgtgccgg ccggccgcga
     2161 gaatcttccg cgcgcccagg gcgcgggcac acatcgcgaa cgccaggccc accggtcccg
     2221 tgcccaggat cagaacgtcc atgcccggct gaaaccgcgc ccggcgcaat gcggagagcg
     2281 tctccttgag cgtgatcagc accgtcgcct cgttggcatc catctccagg ggaataggct
     2341 gctgcatcgc cgccgatccc gcggtcggcc gtgccgatgg gtcatcggcg cgcagcgcct
     2401 cgatatcggt gaggaggcca tactccgcga aaccgcccca gccgatgcgc ggcgcgcctt
     2461 ccatccctgc cgggtaggcc gccccagcac gcaggacgcg gtcgcccacg cggaacttgc
     2521 ggcaccgctc gccggtggcg atcacctcgc cgacgccttc atgccccaga atgaacggca
     2581 taggcgggcc aaaagggagc gtgccatgaa tcaggtgcgt atcggtgccg cggcagaagc
     2641 tacacgccag cgtgcgcgtc agcgcgtcgt agggccccag cttcgggcgg ggcacctcgc
     2701 gcagctccac ccgacccggc gcggtcacca tggcggcaag catcgtttcg ctcatcggag
     2761 tcctcctgga attggaattc cgagatacgg aatccgagcc cgggcataga gacccacggt
     2821 tcgaatgccg gacctctccg gcccctttca tccgtgggat cggggccggg ctcatcccag
     2881 caggggcagg agtgtctcgg cactcacccg catcgcggtt tcgctatccg ggtgctggta
     2941 ggcctccacc gaaacgcacc cgtcatagcc ggcttcgcgc agcgccccca ggatcgcggg
     3001 gaaatcgagg tggcccattc ctggcgccag acggttctcc tcgcagaggt gaacatggtg
     3061 caggtgctca cgcgcggcta ccagcgacga tgggatcgtc tccccttcgg cgtgcatgtg
     3121 ccctgtatcg agcatgaggc caaggccggg gtggttcagc gcgcgcacca gagccagacc
     3181 ttcttccgtg gtccgcgccc aatcggcggt tcgccgctgg atcggctcca ggaggacgcg
     3241 gatcccgagg gatgccgcca ggtccgcggc acccagcagc gccgtgcgtc cccactccag
     3301 cgcgacctcg tccgggacgc cgccgccgag gcgaccgcgc agccggccca cgttcacatc
     3361 gacgcccaaa cgcgcagcca ggcggatggc cgcgcgcatg cgcttcccgg cctcagcgcg
     3421 caccgcgacg cgcgcatccg tgaaagagag gccgtcttcc ccatatacct cgccggtgca
     3481 gaccagacag atggggaggt gatactctcg cgacagggct tccagatagg cagccggcac
     3541 gcgctctggg tcgcgcacca tcaactcgac agcggagtag ccgaggcgag caagctgcgc
     3601 gaaaccctcg cgcaggtcac cccgaaacgc ggtgacatcg ggcgtgtgga cctctggggt
     3661 cgccacctgg taggccagtc tcactcgctc tctcccgagc gaagaagtcc gtactccttc
     3721 atcttattgc gcagagtctt ccggtcgatg ccgagcacga gcgcggttcg gctttggttg
     3781 ccgcccagtc gctcgagcgt atccaggatc gcttctttct caatctcctt cagagttcgc
     3841 agcgggcgct ccccgtctgc gagcgatgag gaatacgccg cagggcgagc cgtggagagg
     3901 ccctgccggg agggcaggaa cagctcgccg acatcgattc gctcgtcacg cgtgaggatg
     3961 acggcgcgct cgacgcagtt ctccaactcc cggatgttgc cgggccagtc gtaactctga
     4021 aggagctcga gcgcctcata ggtgaatccg gtaacctgct tccccgtctc ttttcgatag
     4081 cgctcgagga agtggttggc cagggtgggg atatcctccg ctcgctcgcg aagcggcggg
     4141 aggtgaatcg ggatcacatt gaggcggtag tagaggtcct ggcggaaggt gccctcgcgg
     4201 atcatcgctt cgaggtcgcg gtgggtggca gcgatcacgc gcacatccac gcggatcgtc
     4261 tccgtccctc caacacgctc gaactcgcgc tcctgaagca cgcgcaggag cttcacctgc
     4321 accggcaggc tgatatcgcc gatctcatcg agcaggagtg tgccaccgtg agcgcgctcg
     4381 aaccggccga tatgccgcga cagcgcgtgc gtgaatgcgc ccttctcgtg gccgaaaagc
     4441 tcgctttcga ggagcgtttc cggaagagca ccacagttga ccttcagaaa cggtttctcg
     4501 gcgcgcccac tctggtagtg aatcgcccgg gccagaaact ccttgcccgt gccggtctca
     4561 cccgtgatga gcacggtggc gttgctctcc gccacctgcg tcgccatcag gtaggcctgc
     4621 tgcacctcgg gggtcatgcc gatgacgttg tcaaagtcgt agtgcgcctt cagctccgag
     4681 tgaaggtagc gattctcatc gatcagcagc cggcggtcca tggcgcgcgc cacgatccgc
     4741 tccagctccc cgaggtcgaa cggcttgcga atatactcgt aggcaccctg cttcagcgcc
     4801 tcgatggcat ctgccatcgt cgcgttcacc gtcacgatga tcacgggaat gtcaggccgg
     4861 cgctttttga taaagcgcag gacatccatg ccgttcagcc gggggaggcc aatgtccaga
     4921 atgacaacgg cgtactcctg ctgctcgacc ttgtttaccg cctccacacc gtcctcggca
     4981 gtctctacgc agtagcccgc ctgagagagg gcatcgacaa ggacctgccg gaggaccggc
     5041 tcgtcttcca ctgctaggat cgtgcgttgg gctggcatct tctctctcaa tcaatcgcgc
     5101 gtctttccat tatacacgcc tggccgctgc ttgtaaatgc tcgccccgcc tccgcagccg
     5161 gtcgaggagg agaggtgcgc cctcgcgcgg aaaagccctc ggaggagata agacgcgccg
     5221 ggaacccgtg gtggcctgca tgaaccggca gatgatcccc cttcggcacc ccctggatgg
     5281 aggcacactc ccccactctg ccgacgatgg catctctggt tggcgcatcc ggccaagcgg
     5341 gtatgctctc cgggagagcg gtgtctcaat agggtaaagg aggtcgtgat gaagaaacga
     5401 gcaactctcc tgctggcggc ggttgccctc tttgcggtgg caatggcgcc cccggccagc
     5461 ggggcagtgg aacggcccga tcaggatctc ctgatcgtct ctgggagggg cgaagcacga
     5521 gtcaccccca acatcgccgt ggttcggttg ggcgttgagt cgcgctcggc ccgagccgcg
     5581 gaggcgatcc aggatgcgga ccgccggatg aatcaggtgc tccaggcgat tcgcgcgcag
     5641 aacattcccg agcgcaacat tcagacagta atctacaacg tctcccggca gtatcggccg
     5701 cgtc
//
LOCUS       DAOSCV010000162         5663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9021_length_5663_cov_3.307418, whole genome shotgun sequence.
ACCESSION   DAOSCV010000162 DAOSCV010000000
VERSION     DAOSCV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9021_length_5663_cov_3.307418"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLY56_05385"
     CDS             complement(<1..390)
                     /locus_tag="PLY56_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20945.1"
                     /translation="MRVPLRVLMVEDSEDDALLLARCLRQGGYDVTWQRVDSAKAMAE
                     ALAGQTWDVILADYTLPGFGGRDALRLVTERGLDIPFIVVSGTIGEEAAVDIMKAGAH
                     DFVRKGNLARLVPVENTAMAERTVIQWD"
     gene            complement(387..836)
                     /locus_tag="PLY56_05390"
     CDS             complement(387..836)
                     /locus_tag="PLY56_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ20946.1"
                     /translation="MMTEKVILLVEDNPDDIELTLRALRKNNIANKVVVARDGVEALD
                     YLFGTGAYAGRDIDGQPQVVLLDLKLPRMDGLEVLKRIRADARTKLVPVVILTSSREE
                     QDLIEGYRSGANSYIRKPVDFDQFTEATRQLGLYWLVLNEPPPKEGR"
     gene            complement(864..3137)
                     /locus_tag="PLY56_05395"
     CDS             complement(864..3137)
                     /locus_tag="PLY56_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ20947.1"
                     /translation="MTSAVDRWERTSSEAPAAVPIAAHRARSLRRAAGVLGLTAAGIG
                     VLTLAGWLLGSPALTSVVPEAGATKANTSVSLFLIGVSLWLLSDPGHHRVPRGVGKLL
                     ALTAASVGAVTLGEYFFGWDLGIDQLLFREPPGAPLTSHPGRMAPITALSLLLLGVAL
                     LLLNRQTRIRALAPWLALVTIIAAIFALATHAFIAGEVFQAESYTVVALPTAIAIFLL
                     AVGVLLAQPEHRLVAIVASDTPGGFAARRLLPVAVIVPLLLAWLRLEGQRAGWYGVGL
                     GLAFLTTASITIIMCLVWWNASLLYNADIQRAAAEEQVRRSRDELEERVNERTAELAQ
                     VNQVLRAQVEERDKAQRALQEERDFVSAILDVVGALVVVLDREGRIIRFNRACEQVTG
                     YSFAEVEGRLFWDLFLLPEEVDGVRAVFDALTAGQFPNRHENYWRTRSGGRRLIAWSN
                     SVLLHEGEVRHVIATGIDITELRAAEDALNALNAELEQRVATRTAELRNAVERLEAEI
                     RERQRAEEQNARLTEDLRQQAAELRAANEELESFSYSVSHDLRAPLRSIDGFSQAVLE
                     DYADKLDETGQDYLRRVRRASQRMGQLIDDILKLSRVTRAEMRRETVDLSALARAISE
                     ELQQTEPDRRVEWHIAEGITAEGDPTLLRVVLDNLLGNAWKFTAHQPSPRIEFGARQE
                     GGICVYYVRDNGVGFDPAYADKLFQAFQRLHTADEFTGSGIGLATVRRIVRRHGGRVW
                     AEGAVGKGATFSFTLGS"
     gene            complement(3339..4268)
                     /locus_tag="PLY56_05400"
     CDS             complement(3339..4268)
                     /locus_tag="PLY56_05400"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOJ20948.1"
                     /translation="MHVISASRRTDIPAFYSQWFMNRIRAGSCLVANPFSGRIHRVSL
                     NPAEVIALLFWTRNPAPLLPHLDELERRGFRCAFEVTLTGYGRDLDRDGPAPERVIEL
                     IRRLAGRIGPEFIHWRYDPIILTGQMTADDHRRRFARLCAALAGNVRGCRISFLQLYR
                     KNRARVEQAAEQGGYTYGYIPVGSVAPPRRGRELSVEERRELADALGEIAASHGIPVQ
                     SCCSELLVNPLSNVHQAHCVDRNVITALRPDLELDLKPKPTRAGCGCYASIDIGAYDT
                     CAHACAYCYATRDPAVARAYLRRHDPNAEMLGA"
     gene            complement(4349..4561)
                     /gene="rpsR"
                     /locus_tag="PLY56_05405"
     CDS             complement(4349..4561)
                     /gene="rpsR"
                     /locus_tag="PLY56_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ20949.1"
                     /translation="MQKKSAKGKFRRTKRKVCSFCVDKVSQIDYKAVTRIRRYVSDRG
                     KILPRRVTGTCAKHQRMVALLPFVAE"
     gene            complement(4582..5007)
                     /locus_tag="PLY56_05410"
     CDS             complement(4582..5007)
                     /locus_tag="PLY56_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ20950.1"
                     /translation="MALNRVVLIGRLTRDPELKYTPSGLPVANFTLAVDRNQAPNAQG
                     EKETDFIPCVAWRQQAEFAANYLGKGRLVAVEGRIQVRKWQGQDGQTRWATEVICDRV
                     QGLDRPRQDGEGVPGGGGIPDAGFGGQDAMDLDDPFSEP"
     gene            complement(5042..5329)
                     /gene="rpsF"
                     /locus_tag="PLY56_05415"
     CDS             complement(5042..5329)
                     /gene="rpsF"
                     /locus_tag="PLY56_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ20951.1"
                     /translation="MRDYELTYIIDPALEEEQVNALVERFSALVTSNGGEVANIDRWE
                     KRKLAYEINGKREGLYVIMTFRGTPELRSELDRQLRITEGVIRHLIVRPEE"
BASE COUNT         1069 a         1903 c         1690 g         1001 t
ORIGIN      
        1 gtcccactgg atcaccgtgc gctcggccat cgccgtgttt tcgaccggca ccaaccgcgc
       61 caggttaccc ttccggacaa agtcgtgagc gcccgccttc atgatgtcga cggcggcttc
      121 ctcgccaatt gtcccggaga caacaataaa cgggatatcc agaccgcgct cggtcaccag
      181 gcgcaaggca tcccggccgc cgaagcccgg caaggtgtag tcggccagga tcacgtccca
      241 cgtctgcccg gcaagcgcct cggccattgc cttggccgaa tcgacccgct gccaggtgac
      301 gtcgtagccc ccttggcgaa ggcagcgcgc caggaggagg gcgtcatcct ccgagtcttc
      361 caccatcaag acgcgcagcg ggaccctcat cggccctcct ttggtggcgg ctcattcaac
      421 acgagccagt agaggccgag ttgtcgggtc gcctcggtga attggtcaaa atcgaccggc
      481 ttgcgaatgt agctgttggc gccggagcgg taaccctcga tcaaatcctg ttcctctcgc
      541 gacgatgtca ggatcaccac tggcaccagc ttcgtgcgcg catcagcacg gatgcgcttc
      601 agcacctcca acccatccat gcgcggcagc ttcagatcaa gcaacactac ctgtggctgc
      661 ccatcgatgt ctcgaccggc gtatgcccct gtgccaaaaa ggtaatccaa cgcctccacg
      721 ccatcacggg ccaccaccac cttgttggcg atgttgttct tgcgcagcgc ccgcagcgtc
      781 agctcaatat cgtcggggtt gtcttcgacc aacaagatta ctttctccgt catcacccca
      841 caccccttcg tagggatatc cacctaactt ccgagcgtga aggagaaggt tgctcccttc
      901 ccgacagccc cctctgccca gacgcgccca ccatgccggc ggacaattcg acgcacggtc
      961 gccaacccga tgccgcttcc ggtgaactcg tcggcagtat ggagccgctg aaacgcctga
     1021 aatagcttat cggcataggc cggatcgaac ccaacaccat tatctcgcac gtagtagacg
     1081 catattcccc cttcctgtcg ggcgccgaac tcgatccgag gggatggttg atgcgccgta
     1141 aacttccagg cgttcccgag gaggttatcg agcacaaccc gcaacagcgt gggatcgccc
     1201 tcggccgtga taccctcggc gatgtgccac tccacccggc ggtctggctc ggtctgctgg
     1261 agctcctccg agatcgctcg ggccagggca gagagatcca ccgtctcgcg gcgcatctcg
     1321 gcacgcgtga cgcgcgacag cttgagaatg tcatcaatca actggcccat gcgctggctt
     1381 gcgcgacgaa cacgccggag gtaatcctga ccagtctcat ctagcttatc ggcataatcc
     1441 tccaacacgg cctggctgaa gccatcgatg ctccggagcg gggcacgcag gtcgtgcgaa
     1501 accgagtagc tgaacgattc tagctcctcg ttcgcagcgc gcagctccgc agcttgttgg
     1561 cgcaggtcct cagtgagccg tgcattctgc tcctcggcac gctggcgctc gcggatctca
     1621 gcctcgaggc gctcgacggc gtttcggagt tcggcggtcc gcgtcgcgac acgctgctcc
     1681 agttcggcgt tcagggcatt gagcgcatcc tccgctgccc ggagctcggt gatgtcgatc
     1741 ccggtggcaa tcacgtggcg cacctcgcct tcatggagga gcacactgtt ggaccaggcg
     1801 atgaggcggc ggccgccgct tcgagtccgc cagtagttct cgtgcctgtt cggaaactgc
     1861 cctgccgtga gagcatcgaa gacggcgcgc accccatcca cttcctcggg tagcaggaag
     1921 agatcccaga agaggcggcc ttccacctcc gcgaaggaat agccggtgac ctgctcgcaa
     1981 gcccggttga agcggatgat ccggccttcc cgatccagga cgaccaccag tgcccccaca
     2041 acatcgagga tggccgagac gaaatcgcgc tcctcttgca gggcgcgctg cgccttgtca
     2101 cgctcctcaa cctgagctcg caacacctgg ttcacctggg ccagttcggc cgtccgctca
     2161 ttgacgcgct cttccagctc gtcgcgcgag cggcgcacct gctcctctgc cgcggcgcgc
     2221 tggatatcgg cattgtagag gaggctggcg ttccaccaga ccaaacacat gatgatcgtg
     2281 atgctcgccg tcgtgaggaa cgccaggccg aggcccaccc cataccagcc ggcgcgctgg
     2341 ccttcgaggc gcagccacgc cagcagcagc ggcacgatca ccgccacggg cagcaggcgc
     2401 cgggccgcaa agcccccagg ggtatcactg gcgacgatgg ccaccaaccg gtgctccggt
     2461 tgtgctagca ggacgcccac cgcgagcagg aagatcgcaa tggcagtcgg gagcgccacc
     2521 accgtgtagg attctgcttg aaacacctca cccgcgataa acgcgtgcgt ggccaaggcg
     2581 aagatcgccg cgatgatggt gaccagcgcc agccacggcg ccagcgcccg gatacgcgtc
     2641 tgcctattca gaaggagaag cgccaccccg aggaggagca aactgagggc agtgatcggc
     2701 gccattcgcc ccggatggga ggtgagcggc gcgcccggcg gttcacggaa gaggagctga
     2761 tcgatcccca gatcccagcc aaagaagtat tcgccaagcg tcaccgcccc gacagaagct
     2821 gcagtgagcg ccagtagctt cccaaccccc cgcggaaccc ggtggtggcc cggatcgctg
     2881 aggagccata gcgacactcc gatgaggaaa aggctcacgc tcgtgttggc ctttgtcgcc
     2941 ccggcctctg gcaccacgct ggtcagggca ggtgacccca gtagccaacc agctagcgtc
     3001 agcacgccga tgcccgcggc tgtcaggccg agcactcccg cagcccggcg aagcgaccgc
     3061 gctcgatggg cggcgatggg caccgcagcg ggcgcctcgg aactcgtcct ctcccagcgg
     3121 tcgacggcac tggtcatcca ctcctgcctt tctgcaggaa gacaccctac tctaccctct
     3181 ggattcctgc ctcaaaccga tgccatgcgc cgagcgcgga aagcgtggag cggcatcccg
     3241 atggctacgg gggatccatc cgtgaggtca cgcgagctgc tcacgagcgc cctcgacatc
     3301 gtagaagcgt tcaaccggcg cttggcctga tggtcctctc acgcgccaag catctccgcg
     3361 ttcggatcgt ggcggcgcag gtaggcacgg gcgaccgcgg gatcgcgcgt ggcatagcaa
     3421 taggcacagg catgggcgca ggtatcatag gcgccgatat cgatggaggc atagcagcca
     3481 cagcccgcgc gcgtcggctt cggcttgaga tccagctcta gatccgggcg cagcgccgtg
     3541 atcacattcc gatcaacgca atgcgcctgg tgcacattcg agagggggtt caccagcagc
     3601 tcgctgcagc agctttgcac cggtatgcca tgggaagccg cgatctcgcc cagagcatcc
     3661 gccagctcgc gccgctcctc tactgatagc tcgcgccccc ggcggggcgg cgccacgctc
     3721 cccacaggaa tgtagccgta ggtgtatcct ccctgctccg cagcctgctc cacgcgcgct
     3781 cgattcttcc ggtagagttg caggaatgag atgcggcagc cccggacgtt cccggccagg
     3841 gccgcgcaga ggcgcgcgaa gcggcggcgg tgatcatcgg ctgtcatctg ccccgtcaag
     3901 ataatcggat catagcgcca gtgaataaac tccgggccaa ttcgcccggc caagcggcgg
     3961 atgagctcga tcaccctctc tggcgcgggt ccatccctgt ccaggtcgcg cccgtagccg
     4021 gtgagggtca cctcaaatgc gcagcgaaag ccgcggcgct ccagctcgtc gaggtgggga
     4081 agcaacggcg ccggattgcg agtccagaag aggagggcaa tgacctctgc cgggttgagg
     4141 gaaacgcggt gtattcgtcc ggagaagggg ttggccacaa ggcaggaacc ggcgcggatc
     4201 cggttcatga accactggct gtagaacgcc gggatatcgg tgcgccggct cgcggagatg
     4261 acgtgcatgg cgttttcgag gcgatgcccg gcccgaagtg cgaaagggcc ggcgaaacaa
     4321 tgctcgccgg ccctttcgtg cgcacttcct actcggcaac aaacggaagg agcgccacca
     4381 tgcgctggtg cttggcgcac gtccctgtca cccgacgcgg aagaatcttc ccgcggtcgg
     4441 agacatagcg ccggatccgt gtcactgcct tgtagtcgat ctgggaaacc ttatccacgc
     4501 agaagctgca taccttgcgc tttgtgcggc ggaacttccc cttcgcgctc ttcttctgca
     4561 tcgaccaacc tcctcatcga cttatggctc gctgaagggg tcatccagat ccatggcatc
     4621 ctgcccgccg aagccagcgt ccggaatgcc accacctccg ggaaccccct cgccatcctg
     4681 gcgaggccgg tcgagaccct gcacacggtc acaaatcacc tcggtagccc accgtgtctg
     4741 gccatcctga ccctgccact tgcggacctg gatgcggcct tccacggcga cgaggcgtcc
     4801 cttgcccagg tagttcgcag caaactccgc ttgctggcgc caagcgacgc acgggatgaa
     4861 atccgtctcc ttctcgccct gagcgttcgg cgcctggttg cgatccaccg caagggtgaa
     4921 gttcgcgact ggcagcccac taggcgtgta cttgagttcc ggatcccgag tcagtctgcc
     4981 gatcaagaca acccgattca gcgccatttg cgtttcctcc tcttgaaccg aacctacgcg
     5041 ctcactcttc gggtcgcacg atcagatgcc ggatcacacc ctcggtgatg cgcagctggc
     5101 ggtcgagttc ggatcgtagc tccggcgtgc cccgaaacgt catgatgacg tagagacctt
     5161 cgcgcttgcc attgatttcg taggcgagct ttctcttctc ccaccggtca atattagcga
     5221 cctcaccgcc attgctggtc actagtgcgg agaaacgctc gacgagggca ttgacctgct
     5281 cctcctcgag ggcaggatca atgatatacg taagctcgta atctctcacg cggtatcacc
     5341 tcccttcgga ctaggcggct ccgagacgat gccacggagc agggtaggct cgtgagtagc
     5401 caccgtccgg caatagggcc agacgccata ctgtccaaga tgattatacc atatggagct
     5461 gtcacaggca atagcctgtt tgcccattcc agaccgtgtt gtccggcact cgccttttct
     5521 caccacgcac gggagcgttc cgcgatggcg ccacccaagg gcgtgtctgg cccgttggcg
     5581 tttgactctc aggagggcga tcggtataat ggcagaagga ggtgcctggg aatcgtgata
     5641 caaacgcacg aggaagcgga tag
//
LOCUS       DAOSCV010000163         5660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9027_length_5660_cov_3.810705, whole genome shotgun sequence.
ACCESSION   DAOSCV010000163 DAOSCV010000000
VERSION     DAOSCV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9027_length_5660_cov_3.810705"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLY56_05420"
     CDS             <1..453
                     /locus_tag="PLY56_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20952.1"
                     /translation="DVVIRVHNPYGMDAGMPGMPGMPGMPGGAPTQSSAPPTPKWIYE
                     RSRDGATFVFGFDEDGRVNLVVVGDDTPYSITRGGRKPPFAGAKTAKGIGLGADFKSV
                     ILAYGFPEATEVLGDEVLLKYFERHGVAFTFRQNFMKVTSIAIKSPEE"
     gene            554..1246
                     /locus_tag="PLY56_05425"
     CDS             554..1246
                     /locus_tag="PLY56_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20953.1"
                     /translation="MKAVWLAMAALVCAGGIAVAGPAPGGGMEIQLAGIQLGKPAVTV
                     VKAYGNPRRVQVGTVTSPGQSGGAGGAGMPGMPGGEMGGMPGGAPPMGAPGMPGPEMG
                     GMPGAPSQPAQAPQTIHWIYEREREGVVYVFGFDEEGTVVAITVGDGLEDSVNRGGTR
                     RPFAGARTSRGIKLGDPFREVIRAYGYPETQQNAGDEVVLTYYEKAGVAFSMKQGVMR
                     VTSITIRDMGAH"
     gene            1274..3097
                     /gene="dnaG"
                     /locus_tag="PLY56_05430"
     CDS             1274..3097
                     /gene="dnaG"
                     /locus_tag="PLY56_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ20954.1"
                     /translation="MSIAEETIEAIRARADIAAVVSQYVTLKRRGRNLVGLCPFHTEK
                     TPSFSVFPERQSWHCFGCGEGGNVFTFLMRIDHLTFPEAVERLGQQVGIPVERSRENA
                     ARRSLRQQILEANQAAAEFFARQLEKAPAAQEVLRRRGLTADSVKALGIGYAPAGWET
                     LSTALGRSVPREVLAQAGLVREREGGGYYDYFRDRLIFPIRDVEGRVLGFGGRALGDA
                     QPKYLNSPETPVFDKSRTLYGLDRARRAIADTGMAIVVEGYMDAAMAQQEGVLNVVAT
                     LGTALGATHLEVLRRYAPRVVLAYDGDAAGAAAAERSLPIFESVDMEGHILILPSGQD
                     PDEFIRKQGVDAFRQAAASALPLVEFQLRRLIERGDRSTPEGRAALIRAVAPVLAAIR
                     QHPKREEYVRRLAEEWSLGQPFGMQHIADALWREVRQASVRSGSAARPAAGPSHTAAP
                     QAPADRDQLVLPPRIALAEQYVLQGLLCGATTSDQVFDVLTPEDFQVPAHRALARILW
                     LMDEEVKDPVDAIRRSGGEDSGVLEGLASHMLAEQEKLPVTTKMVTDSVWAIKETKLR
                     ARISELQLRLAKLQSEPGEERTRVEKELQELEKELRDRKAG"
     gene            3121..4236
                     /gene="rpoD"
                     /locus_tag="PLY56_05435"
     CDS             3121..4236
                     /gene="rpoD"
                     /locus_tag="PLY56_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOJ20955.1"
                     /translation="MPVESVEQSKEMRRLIDKGKKKGTLTYDEISETLYQDENLDEGQ
                     IEDILQTFADEGIQIVNKMHGIPEVPESTPAPAEQTAESEIRDEDLAAVEGIPIDDSV
                     RMYLREIGKVPLLTLEEETSLAKRIDQGDEEAKRKLTEANLRLVVSIAKKYTGRGMSF
                     LDLIQEGNIGLIRAVEKFDYHKGYKFSTYATWWIRQAITRAIADQGRTIRIPVHMVET
                     INRLVKTNRQLLQELGREPTLNELADALEMPVERVAEIIKIAPEPLSLETPIGEEEDS
                     HLGDFIEDQEALSPADAASNMILREQIENVLAELTDREREVLKLRFGLADGSPHTLEE
                     VGSIFKVTRERIRQIEAKALKKLRHPSRKQKLQDYLD"
     gene            complement(4487..5392)
                     /locus_tag="PLY56_05440"
     CDS             complement(4487..5392)
                     /locus_tag="PLY56_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ20956.1"
                     /translation="MIEEERLLLCLAHVPGLGERGLDALLRSGIPLARLFSLPAEELQ
                     RRFGLTARVAAAIAGGLDAIWARVAPLARTVAEQGIHLLRRGHPGYPAGLAECEGAPP
                     LLFAYGNVELPMRPSLALLSSNGESRVGLERTSNLAFACARAGVAVVAGHNRPSYRAA
                     VSGAKAHGGGRIIVLDRGLLDAFQSDFNRDLFPSARIWGYAFDRERSVVLSPYPLDAH
                     FAGLRNRRRDRLVSLLASAIVAVEIRGGGVMEGECRQARARGTPVFVCAGPDIPDGNR
                     ALLADGFPPLPEEDPIARVLCNQAP"
BASE COUNT         1061 a         1730 c         1926 g          943 t
ORIGIN      
        1 gacgtggtga tccgcgtgca caacccctac gggatggatg cgggcatgcc cggtatgcct
       61 ggaatgcctg gaatgcccgg cggcgcgccc actcaaagct cggcgccacc cactcccaag
      121 tggatttacg agcgctctcg cgatggcgcc accttcgtct ttggctttga tgaggatgga
      181 cgcgtcaacc tggtcgtggt tggagacgac acgccctact ccatcacccg cggcgggcga
      241 aagcccccct tcgccggggc gaagaccgcg aaggggatcg gtctgggcgc ggatttcaag
      301 agcgtgatcc tcgcctacgg cttccccgag gccacggagg tgctggggga tgaggtactc
      361 ctgaagtact tcgaacggca tggcgtcgct ttcaccttcc ggcagaactt catgaaggtg
      421 acctcgattg ctatcaagtc gccggaggag tgaaagcaga atggcaggcg cgcgaccccg
      481 ggtcacgcga cgagcgtgtg ccttgccacg ggttgatggg ggcatagtcc ggatggcgaa
      541 cgaggtgaga gcgatgaaag ctgtatggtt ggcgatggcg gcgcttgttt gcgcaggtgg
      601 catcgcggtc gcagggccgg cgccaggcgg cgggatggag atccaactcg cagggatcca
      661 gctcgggaag ccagcggtca cggtcgtgaa ggcatacggc aatccgcggc gtgtccaggt
      721 gggcacggtg acgtcgccgg gtcagtcggg cggagccggt ggcgcaggca tgcccggcat
      781 gcccggtggc gagatgggcg ggatgcccgg tggggctccg ccgatgggcg cccctggaat
      841 gccaggtccg gagatgggcg gtatgcccgg tgcaccatcg cagccggcgc aggctcccca
      901 aaccatccac tggatctatg agcgcgagcg cgaaggcgtc gtctacgtct tcggcttcga
      961 tgaagagggc actgtggtcg cgatcaccgt cggggatggc ctggaagact ccgtcaaccg
     1021 aggtggtacc cgccgcccct tcgcgggggc gaggaccagc cgcggcatca agctggggga
     1081 tcccttccgc gaggtgatcc gtgcctacgg ctatcccgag acgcagcaga acgcggggga
     1141 cgaggtcgtc ctcacctatt acgagaaggc aggggtcgcc ttctcgatga agcagggtgt
     1201 catgcgtgtg acctccatca cgatccgcga tatgggtgcg cattaggcgg atagttgtgg
     1261 ggagagcgac gcgttgtcca tcgctgagga gacgattgaa gcgattcgcg cgcgtgcgga
     1321 tatcgccgcc gtggtatcgc agtatgtgac cttgaaaaga cgggggcgca atcttgttgg
     1381 cctgtgcccc tttcatacgg aaaagacacc ctcgttcagc gtttttccgg agaggcaaag
     1441 ctggcattgc ttcgggtgtg gtgagggggg caacgtcttc accttcctga tgcgcatcga
     1501 ccatctcacc ttcccggaag cggtggagcg tctgggccag caagtgggca tcccggttga
     1561 acggagccgg gagaatgccg cgcgccgaag cctgcggcag cagatccttg aagccaacca
     1621 ggcggcggcg gagttcttcg cccgacagct ggagaaggcg cccgccgccc aagaggtgtt
     1681 gcgccggcga ggcctcaccg cagatagcgt gaaggccctc ggcatcggct acgcgccagc
     1741 ggggtgggag acgctctcga ccgcgctggg ccgctccgtg ccacgcgaag tgctggcgca
     1801 ggcgggtttg gtgcgcgaga gggagggcgg tggctactac gactattttc gggaccgcct
     1861 catcttcccg atccgagacg tggagggacg tgtcctggga ttcggcgggc gcgctctggg
     1921 cgatgctcag cccaagtact tgaactcgcc ggagaccccc gttttcgaca agagccgcac
     1981 gctctacggg ttggatcgtg cccgtcgggc gattgccgat accggaatgg ccattgtcgt
     2041 cgagggctac atggacgcgg cgatggcgca gcaggaaggc gtgctgaacg tcgtcgccac
     2101 gctgggtacg gccctgggcg ccacgcatct ggaggtgttg cgccgatatg cgccacgcgt
     2161 ggtgctggcg tatgatggcg atgccgcggg agccgcggcc gccgagcgca gcctgccgat
     2221 ctttgaaagc gttgatatgg agggccatat cctgatcctc ccctccggac aggacccgga
     2281 tgagttcatc cggaagcagg gtgtcgatgc cttccgacag gccgcggcga gcgcgctacc
     2341 ccttgtggag ttccagcttc gccgcctcat agagcgcggc gatcggagca cgcccgaggg
     2401 gcgggccgcc ctgattcgcg cggtagcgcc tgtgctcgcg gcgattaggc agcatcccaa
     2461 acgggaagaa tacgtgaggc gcctggccga ggagtggagt ctgggccaac cgttcgggat
     2521 gcagcacatc gccgacgctc tctggcgcga agtgcgccag gcctccgtgc gttccggatc
     2581 cgccgctcgg cctgctgccg ggccgtcgca caccgccgcg ccacaggcgc cggcggaccg
     2641 cgaccagctc gtgctcccgc cccgcatcgc gctggccgag cagtacgtcc tccagggcct
     2701 gctctgtggt gcgaccacgt ctgatcaggt gttcgatgtg ttgacgccgg aagatttcca
     2761 ggtgcccgca caccgcgcgt tggcgcgtat cctatggctg atggatgaag aagtgaagga
     2821 cccggtcgat gcgatccggc gttcaggagg ggaagattct ggagtgctag agggactggc
     2881 ctctcatatg ctggcggaac aggagaagct gcctgtaaca acgaaaatgg tgacggattc
     2941 tgtgtgggct atcaaggaga caaagctccg ggcgcgaatc agtgaactac agcttcggct
     3001 ggccaagttg cagtcggagc caggtgagga gcgtacccga gtcgagaagg agttgcagga
     3061 actcgaaaag gagttgaggg atcggaaagc ggggtagccg cgaggagggg gtgagacgag
     3121 atgccagtgg agagtgttga gcagtccaaa gagatgcgac ggctcattga caaaggaaaa
     3181 aagaagggta cgctcacgta tgatgagatt agcgaaacct tgtaccagga tgagaacctg
     3241 gacgagggtc aaatagaaga tattctccag acgtttgcgg atgaggggat ccagatcgtg
     3301 aataagatgc atggcatccc ggaggtcccc gagagcacgc cggcccccgc ggaacagact
     3361 gctgagtcgg agatacgaga cgaggatctg gctgccgtgg aagggattcc aatcgatgat
     3421 tcggtgcgga tgtatctgcg cgagatcggc aaagttccgc tcttgacgct ggaggaggag
     3481 acgtcgctgg ccaaacgtat cgaccagggc gacgaagagg cgaagcgcaa gctcaccgag
     3541 gccaatctcc gcctggtggt cagcatcgcc aagaagtaca ctgggcgtgg catgtcgttc
     3601 ctggatctta tccaggaagg gaatatcggt ctcatccgtg ccgtcgagaa gttcgactac
     3661 cataagggct acaagtttag cacctacgcc acctggtgga tccggcaggc cattacgcgc
     3721 gcgatcgctg atcaagggcg taccattcgc attccggtgc atatggtcga gacgatcaac
     3781 cgcctcgtga agacgaaccg gcaactgctc caggagctgg gccgcgagcc aacgctcaac
     3841 gagctggccg atgcgttgga gatgccggtg gagcgcgttg ccgagatcat caagatcgcc
     3901 cctgagccgc tctcgctcga gacgccgatt ggcgaggaag aggatagcca cctgggcgac
     3961 ttcatcgagg accaggaggc gctctcgcct gccgacgccg catccaacat gatcctgcgc
     4021 gagcagatcg aaaacgtcct cgccgagctg accgaccgcg aacgtgaggt gctgaagctg
     4081 cgcttcggcc tggccgacgg aagcccgcac acgctcgagg aggtcgggag catcttcaag
     4141 gtcacccgcg agcgcatccg ccagatcgag gcgaaggcgc tcaagaagct gcggcacccc
     4201 agccgcaagc agaagctcca ggattacctg gattagcgct gcggcgccgc accaccgcat
     4261 tcaccgggag acaggtgcct tcgcggtgcc tgtctccata gctatgaggc gcagacgctt
     4321 gccgccagcg ttgactcccc cgctcgcccc ttccgactgc gcatctatcg gactgtacct
     4381 cccgcgaagc catccacgcc gggaagccat gccatcccga agttcaacct gccgggcaac
     4441 acaggcgctg cagcttcgcc ggccagtctc cctgtttgat gcgtctttac ggtgcctgat
     4501 tacatagaac ccgtgcgatc ggatcttcct cggggagcgg ggggaagccg tcggccagga
     4561 gcgcgcgatt tccgtcgggg atgtcgggtc cggcgcacac gaagacaggc gtgccccgcg
     4621 ctcgcgcctg gcggcactcc ccttccatca ccccgcctcc ccggatctca acggccacaa
     4681 tcgcgctcgc cagcagtgac accaggcggt cgcggcgacg attgcgcagg ccagcgaaat
     4741 gcgcatccag cgggtacggc gagagcacga cggagcgctc gcgatcaaac gcgtagcccc
     4801 agatccgcgc tgaggggaag aggtcacggt tgaagtcgct ctgaaacgcg tcgagcaggc
     4861 cccggtccaa cacgatgatc cgcccgccgc cgtgcgcctt ggcgccgctg actgccgcgc
     4921 gatagctcgg ccggttatgt ccggctacaa cggccacgcc tgccctggca caggcaaacg
     4981 ccagattact ggtgcgttcc aggccgacac ggctctcccc gttggaggag aggagcgcca
     5041 ggctcgggcg catcggcagt tcgacattgc cgtaggcaaa gaggaggggc ggcgcgccct
     5101 cgcactcggc gagcccggcc ggatagccag gatgcccgcg gcgcaggagg tgaatcccct
     5161 gctccgccac cgtgcgggcg aggggcgcaa ctcgcgccca gattgcatct agccctccgg
     5221 caatggcggc ggcgacgcgc gcagtcagcc cgaagcggcg ttgcagctcc tccgctggga
     5281 gtgagaagag gcgcgcgagg ggaatgcctg aacggagcaa tgcatccaga ccgcgctccc
     5341 caagccctgg aacatgggca aggcacagta gcaggcgctc ctcttcgatc accgcgccac
     5401 ccccttgctg ccctctcggc cgacgagcgg gcggctgcta gttcggcacc aggcacctga
     5461 ttcctgcagg gagtacaacc gtcaccctac ccacctcctt cggtagcgaa gttgcttcac
     5521 cttcgctggc gcgtcctcat cctgctccct ggcggcatag cttctgggat tccgagataa
     5581 ggagaagacc gcctgcacgc gcggtgctcg aagctccgcg ctgctttccg ccaaggtccc
     5641 ttcggggctt ctcatcccag
//
LOCUS       DAOSCV010000164         5651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9039_length_5651_cov_3.089350, whole genome shotgun sequence.
ACCESSION   DAOSCV010000164 DAOSCV010000000
VERSION     DAOSCV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9039_length_5651_cov_3.089350"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /gene="thpR"
                     /locus_tag="PLY56_05445"
     CDS             complement(<1..418)
                     /gene="thpR"
                     /locus_tag="PLY56_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ20957.1"
                     /translation="MGINQRWSGEGAIRAFVAVPLPEDARSCVEGVQRRLRGFAGVKW
                     VEPENFHVTLRFLGNSPPELVQRLAQTLLRTLSDQKAFSLTLAGVGAFPSARRPQAIW
                     VGVSEGKSRLEELARITEESAVASGFAPEERPFRAHL"
     gene            complement(672..1142)
                     /locus_tag="PLY56_05450"
     CDS             complement(672..1142)
                     /locus_tag="PLY56_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20958.1"
                     /translation="MKWLPLAAAFVTSILLFPGCGGSSATVRMTQQAPVLRSVEITPE
                     DLRFSFDGGKITLRVTVDSERAIHAVTAERSLLNTQKPETKLYPLVPVTGGANVYQTS
                     VDVPANTRPDGQPVHYRLSVFATDVAGVQSDRVEREIRVAAPDASGLPSVPFNP"
     gene            2386..>3198
                     /locus_tag="PLY56_05455"
     CDS             2386..>3198
                     /locus_tag="PLY56_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HOJ20959.1"
                     /translation="MTKKEPETVLQPVGKDEMNLAEYPFTLLTRRVSENQKTIEVQQQ
                     VRTETGEVITQSWVVTGSDRYGLPLAIDEDIYIALMKILKDGGFRDRTIPFTRYQILR
                     ILGKDISKREYDRIQQSLDRLVGTTITSKNAFWDNRTRSYVSKAFHIFESYELYREQP
                     GRKSARSPELPMSYVVLSSFLFESVKSGYVKNLDIEFYLSLRTPLAKRLFRFLDKKAY
                     NNRTFEIGVMRLAEKLPVHDAFPSQVKRRLEEPHKELIEKGFGPQARTGPRGA"
     assembly_gap    3199..3208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3209..3843
                     /locus_tag="PLY56_05460"
     CDS             <3209..3843
                     /locus_tag="PLY56_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20960.1"
                     /translation="LAEVNSARRRDGEEKVIYTFKRPRMNAEETLTPLGDPTVEMLVA
                     RGISRSAAEELAARFSEEHIQKHVDAFDQLCAEESPRIRRNPAGYLRRAIEEDYAIPE
                     PRRPKPTARATSAKSADEKPAGTRVRKRPAGESGAAVRDVEEAPLAPRSREGIDPERL
                     ETLRERARQMVLERYPVLAQRSTSPAFDVMVDACLDEILAVEGPATLAAE"
     gene            3970..4044
                     /locus_tag="PLY56_05465"
     tRNA            3970..4044
                     /locus_tag="PLY56_05465"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4003..4005,aa:Glu,seq:ttc)
     gene            4112..4930
                     /locus_tag="PLY56_05470"
     CDS             4112..4930
                     /locus_tag="PLY56_05470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ20961.1"
                     /translation="MSSLPYELIALDLDMTLLNPQLEISPRNRAAVRRCRDLGVKAVI
                     SSGRMHASTLAYQRALELDTPIISYNGALIKCDTTGEVLLHRRVNLEIAREIVALCER
                     EGLHLNYYLDDTLYVREANRWAQLYAERCAVPFHAVGDLRKLTDRAPTKLLIFAEPER
                     IAELNRRLAPVYGDRAYITPSFADYLEVMPLGVDKGQALAVVARHFGVPQEKTIAFGD
                     ALNDVPALRWAGLGVAMANAKPAVKDAADQIAPHHAEDGVAAVLEALFDLPAGS"
     gene            5476..>5651
                     /locus_tag="PLY56_05475"
     CDS             5476..>5651
                     /locus_tag="PLY56_05475"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOJ20962.1"
                     /translation="MKEDVFICSAARTPIGRFDGNLAEFSAVDLGAVALAAALERAGV
                     DPARVEAATLGNVV"
BASE COUNT         1094 a         1800 c         1753 g          994 t
ORIGIN      
        1 tcaagtgtgc gcggaatggc cgctcctctg gcgcgaagcc gcttgcaacg gcgctttcct
       61 cggtaatgcg cgccagctct tccagacggc tcttcccctc gctcacgccc acccagatcg
      121 cctggggccg acgcgcgctc gggaacgcgc ccacaccagc cagcgtcagc gagaacgcct
      181 tttgatcaga gagggttcga aggagggtct gagcaagacg ctgcacaagc tcgggagggc
      241 tgttgccgag gaaacgcagc gtgacgtgga aattctctgg ttcgacccac ttcactcctg
      301 caaagccgcg cagtcggcgc tggacccctt ccacgcacga ccgcgcatcc tcgggaagag
      361 gcaccgccac gaacgcacgg atcgcccctt cgccgctcca acgctgattt atgcccactg
      421 ccagtcactt cccgtgatac gccggccagg tcatgccctg gcgtgcgccc cgctccccac
      481 caggctggtg tcatccaccg aagcactctc cggcatctcc agcagcctca ggcaggatcg
      541 cgcggcgcct ccgcactcgg agcgtgcagc agcgcgcgct ttggagaacg cgccggcaca
      601 cgcacgagat caatccgatg gcgtgagcgg atctctccgg atcctgacgg aggatcaacc
      661 gtctcgtggt gtcacgggtt gaaaggcacc gatggcagac ccgaagcgtc tggagccgcg
      721 acgcggatct cgcgctcgac ccggtcggat tggacgcccg cgacatctgt tgcgaagacg
      781 gagagcctgt agtgaaccgg ctgtccatcg gggcgcgtgt tggctggcac atcgaccgat
      841 gtctgataca cattcgcccc gccagtgaca ggcacgagag gatagagctt cgtctccggt
      901 ttctgcgtgt tcagaagaga tctttccgcg gtgacggcat ggatagcccg ctcgctgtca
      961 accgtcacgc gaagggttat cttccctcca tcaaagctga aacgaagatc ctcaggggta
     1021 atctcaacac tccggagcac cggcgcctgc tgggtcatgc gaacagtggc gctcgaccct
     1081 ccacatccgg gaaaaagcag gattgatgtg acgaacgctg cggcgagcgg cagccatttc
     1141 atgcagtaca ctcctttccg acgccgtagc gagtgtaacc ctcatctggc ctgtgcgcca
     1201 ggatcacgat caccggctgg cagccaggcg ccggattcgc cgaaccctcg gtgccgacag
     1261 caagaccacc ctatccggga agatacaccg ggtaatccgt ggagagtgcg gcacggccat
     1321 ccggaaaacc ctggccaaag ccggtccagc ccgccctcac gccggatccc cccctggcca
     1381 tctcgatgcc agggcagggc ctctttagca gcgaccactc cagaaggaga atacctgccg
     1441 gagtgccaat cgcctgcgcg tattatagcg ttcactacca cggctgtcaa tgccaagcgc
     1501 tcgcgaattg cgtgcccgca cgcctccacc gcgcacccaa ccgctaatca tgtgttttcg
     1561 aacaagccgc tgtgcagcca caacccgcgc atggatcgac cggtcgtgca gccgcatttt
     1621 gacaagagag tgacaggaat agtgtgctat gagacaactg aggagggggc gtcactgggc
     1681 aatgctgtgt ttcgggacaa gagcgcgcct cgtggcgcct tttcgccagg ctggctcgct
     1741 agatgaggga actcaatagt gtgttttcgg acaactcgaa cgggcacgac gggtcgatcc
     1801 cgcgcacgct tccggcgaga ccggagtccg ccgacgccag atcagcctgc cagcacccgt
     1861 ccagcgacga tcccgcccgc cggggtgccg gagaaacgcc ggaatcttgc tccaaaagcc
     1921 ggcgaacagc cccaaaatag tgtgttttgg agcagtacgc caccatttgc ccccgattcg
     1981 gccccgaaac cgcccccgtc taccgcccgg gtacggtaga atagtgtgtc accgagcaag
     2041 cgccggtcga aggcacactt ctgttccctg tgccttcggg caaggaatac cgtgtttcgg
     2101 ggcaagggaa taccgtgcta ccgagcaggg agtgctgtgc tatgggacaa ttcggccggc
     2161 gaatatagtg tgctttcaga caacgaaagc tgtgttttca agcaaacgag gttttcacga
     2221 acccaataac gacgcggctt ccagccatct tcgccgccgt ctgaatttgc agacagaggc
     2281 cttgatcaac actatctgat cagggctggt ggtgatgctc ctcaattagg aagagggaag
     2341 ctgtcaccct tgtgattccg agtagttcgt acgggagagc gagccatgac gaagaaggag
     2401 cccgagaccg tgctacaacc tgttggcaaa gacgagatga atctggcgga gtatcccttc
     2461 acgctcctga cacggcgcgt ctccgaaaac cagaagacca tcgaggtgca gcaacaggtg
     2521 cgcacagaga ccggcgaggt gattacccag agttgggtgg ttaccggaag cgaccgctat
     2581 gggctgcctc tcgccatcga tgaggatatc tacatcgccc tgatgaagat cctgaaagac
     2641 gggggtttcc gcgaccgaac catccccttc acgcggtatc agatcctgcg gatcctcggc
     2701 aaggatatct cgaagcggga gtacgatcgc atccagcagt cgctggaccg cctcgtcggc
     2761 accacgatca cttcgaaaaa cgccttttgg gacaaccgca cccgctccta cgtgagtaag
     2821 gcgttccata tcttcgaatc gtacgaactg tatcgcgagc agccgggccg aaagagcgcg
     2881 cgttcgccag agctgccgat gagctacgtg gtcctcagct ccttcctctt cgagagcgtc
     2941 aagtcaggct acgtcaagaa cctggatatc gagttttacc tcagcctgcg cacgccactt
     3001 gccaaacgcc tcttccgttt tctggataag aaggcgtaca acaaccggac cttcgagatc
     3061 ggagtgatgc gcctggcaga gaaactccca gttcacgacg cgttcccctc gcaggtgaag
     3121 cgacgcctcg aagagcccca caaggagctg atcgagaagg gcttcggccc gcaggcgcga
     3181 actgggccgc ggggcgccnn nnnnnnnntc ctggccgagg tgaactccgc ccgccgtcgc
     3241 gacggcgagg agaaggtcat ctacaccttc aagcgcccgc ggatgaatgc cgaggaaacg
     3301 ctcactcccc tgggcgatcc caccgtcgag atgctggtcg cgcgcggaat ctcgcgctcg
     3361 gccgccgaag aactggcagc gcggttcagc gaggagcata tccagaagca cgtcgatgcc
     3421 ttcgaccagc tctgcgccga ggagagcccg cgcatccgcc gtaacccggc cggctacctg
     3481 cgccgggcaa tcgaggagga ctacgcgatt cccgagccac gccggccgaa gccgaccgcg
     3541 cgcgctacct ctgccaagag cgccgatgaa aagccggccg ggacgcgcgt ccgcaagcga
     3601 ccggcgggcg agagtggggc ggcagttcgc gacgtcgagg aagccccgct ggctccgcga
     3661 tcgcgcgagg ggatcgatcc cgagcgtctg gagactctgc gcgagcgggc ccgacaaatg
     3721 gtgctggagc ggtatcccgt gctggcccag cgctccacca gtcccgcgtt cgatgtgatg
     3781 gtggatgcgt gtctcgatga gatcctagcc gtcgagggcc ccgcgacgct agccgccgag
     3841 taggcgccgg cctgagagcg gggggtggga aggccgcgtc cggcatcctc acccgcggta
     3901 tcagcgtgga aaatacgttc ttgacccaga cccccgctta tggtaaaata agctgtccgc
     3961 gcggggagcg ccccgttcgt ctagaggtca ggacaccaga ttttcattct ggcggcaggg
     4021 gttcgattcc cctacggggt accatggcga ggccgtcttc ggtggaaggc ggcctttttt
     4081 gtgcgctcgc gccctgaagg agtggattcc agtgtcatcc ctgccctatg agctgatcgc
     4141 gcttgacctc gatatgactc tcctcaaccc ccagttggag atcagcccgc gcaaccgggc
     4201 ggcggtgcgc cggtgtcggg acttgggcgt gaaagcggtg atcagctccg ggcggatgca
     4261 cgcaagtacc ctcgcctatc agcgcgcgct tgagctcgat acccctatca tctcctacaa
     4321 cggcgcgctc atcaaatgcg atacgactgg cgaggttctc ctgcaccggc gggtgaatct
     4381 cgagattgct cgcgagatcg tggcgttgtg cgagcgcgag gggctgcacc tgaattacta
     4441 cctcgacgat acgctctatg tgcgcgaggc gaaccggtgg gcgcagctct acgcggagcg
     4501 gtgcgccgtc cccttccatg ccgtgggcga tttgcggaag ctcaccgacc gtgctcccac
     4561 caagctcctc attttcgcag agccggagcg gatcgcggag ctcaaccgcc gcctggctcc
     4621 tgtctacgga gaccgtgcct acatcacccc ctccttcgcg gactacctgg aggtgatgcc
     4681 actgggcgtg gataagggcc aggcacttgc cgtggtcgcc aggcactttg gcgtccccca
     4741 ggagaagacg attgccttcg gagatgcgct caacgacgtg cccgctctac gctgggccgg
     4801 cctgggtgtt gccatggcga acgcgaagcc cgcggtgaag gatgctgccg accagatcgc
     4861 gccccatcac gccgaggatg gtgttgccgc ggttctggaa gccctctttg atctccccgc
     4921 cgggtcctag accccaagtt tcgcacgcgc agtagtggct gctgcgaatc ccaacgcttc
     4981 ctcacgatac acctgctcca cgcgcctgcg acggccattt cgccgcggga cgccctggca
     5041 cggcattcag atggcctgcg ccgtgccggc aaggtgctct tcccaaggct ggcgtcctgg
     5101 agtataataa ggtgtggagc acgacagaga atgggggcag ggctgtactg cggcagacac
     5161 cccctgggag caagagccat cgccagctgg tctggaggtg gcgatgggac gaagtgtaac
     5221 gtcccctccg gatcggcgga gaagtgcctc tggagccgtg gctgcttggc tccggcacgt
     5281 gcgcctgcac ggcccgcgct cctatgagac cgccacgccc gggacccgtt ggggggtgct
     5341 gaagccacgg ggaagtgggc gcccgagaag ctggatctgc ctgacccagg ttcgcatcat
     5401 agctcggaat gcacgcggcg atgtgccgga gtgccggtcg ctattcgtaa ggatcgaaga
     5461 acaatgcatc tcatcttgaa ggaagacgtc ttcatctgct cggcagcgcg aacgcccatc
     5521 gggcgctttg atggcaatct ggcggagttc agtgccgttg atctgggagc ggttgccctt
     5581 gcggcggcgc tggagcgcgc gggggttgat ccagcgcgcg tagaggcggc cactctgggc
     5641 aacgtggtga a
//
LOCUS       DAOSCV010000165         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9059_length_5640_cov_2.890779, whole genome shotgun sequence.
ACCESSION   DAOSCV010000165 DAOSCV010000000
VERSION     DAOSCV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9059_length_5640_cov_2.890779"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PLY56_05480"
     CDS             complement(<1..1863)
                     /locus_tag="PLY56_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOJ20963.1"
                     /translation="MSDNEFLTIDGKEVRIEGERNLLEVIRKAGIDIPTFCYHSHLSV
                     YGACRLCLVEVEGRGIVGSCSTPPEPGMKVKTTTQEIREIRKIAVELLLANHEKTCPT
                     CSKSASCQLQEIARRLGVDEVRFKSTHQPVPVDRSSPSLVRDPNKCILCGDCVRVCHE
                     IQGIGAIDFAYRGASTAVLPAFGKDLKHVECVYCGQCASVCPTGALTVKSEIDAVWEA
                     LSDPKKKVVAQIAPAVRVAIGEHFGLAPGTVSMGQTVAALKAMGFDQVYDTSFAADLT
                     VIEEATEFLARKEKGERLPIFTSCCPAWVKFAEQYYPSLLPHLSSCRSPQQMFGSIAR
                     EVLPEKLGVENKDLVIVSIMPCTAKKFEARQEKFIQDGIPDVDYVLTTQELGRMIEAQ
                     GLRFSRLEPESLDMPFGFKTGAGVIFGTSGGVTEAVLRYAVEKVSGTRLEGVDFRAVR
                     GEEGLREATVNVAPGVDLKLAIVHGLANARRVAERVMAGECDYDIVEVMSCPGGCIGG
                     AGQPVSRDPGASRLRARGLYDSDKMLEFHKSQENHVVTECYDRHLGEVGGEKAHHLLH
                     TNYTSRRRICDEEIALVNGNGQAKVKVNICVGTSCMVRGSQRLLQSLIDQIEERG"
     gene            complement(1910..3829)
                     /locus_tag="PLY56_05485"
     CDS             complement(1910..3829)
                     /locus_tag="PLY56_05485"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOJ20964.1"
                     /translation="MSVSETMQPVPRPDLDAVAREYWQAARTYERRVIICAGTGCVAN
                     GALKVHAAFEQQIAAAGLSVATELRAETAGGDLRLSKSGCQGFCQQGPLVTIEPEGIL
                     YTRVRPEDVAEIIETTLQGGEVVERLLYTDPLSGKQCRGTGEIPFYTLQHRYVLKACG
                     AIDPEDIREYIHNGGYRAAEAAFTRMTPEEDCEEVTYSGLRGRGGGGFPTGKKWSLTL
                     QQPGPKKYVICNGDEGDPGAFMDRSLMEGNPHSIIEGMMIAARAIGADEGYIYVRAEY
                     PLAVSRVRKALADAEALGILGDNVFGTGHSFRIQVMEGAGAFVCGEETALISSIEGKR
                     GMPTPKPPFPAQSGLFGKPTVINNVETLATIPLILSAGAAEFRAMGTEKSPGTKTFAL
                     TGHVANTGLIEVPFGASLREIVHHIGGGVTDATGAITGEGFKAVQIGGPSGGCLTEAQ
                     LDLPLDYDSLRSVGAMVGSGGLVVMNKQTCMVKIAHFFMQFTQNESCGKCVLCREGTK
                     QMLALLEEIIEGQGTLETLDLLEKVARAVQKGSLCGLGKTAPNPVLSTLRYFRAEYEA
                     HVVQKRCPAGQCKALLVPAIDPALCKGCTLCAKKCPVGAISGDRKMPHAIDPEKCIRC
                     GVCAESCKFGAVTGL"
     gene            complement(3826..4335)
                     /locus_tag="PLY56_05490"
     CDS             complement(3826..4335)
                     /locus_tag="PLY56_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ20965.1"
                     /translation="MALALHSASQQHRKFQRVCEIIDEHDRNPGRLVPILQAVQEEYR
                     YLPEDVLTYIATSLGISPARVYGVATFYAHFALEPKGKYIVRLCDGTACHVKGSIPIL
                     EAIRERLGLKEKQSTTPDMLFTVETVSCLGACGLAPVVVINEQVHGQMTPEKAVEVIE
                     EILASEESR"
     gene            complement(4337..4993)
                     /locus_tag="PLY56_05495"
     CDS             complement(4337..4993)
                     /locus_tag="PLY56_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOJ20966.1"
                     /translation="METGKQKGAPLPTVRRLPTYLRLLRQMEKDGREFVSSTAIADEL
                     HLESIQVRKDLAVTGIEGKCRVGYPVSELIQAIEEFLGWDNTTDAFLVGAGSLGAALM
                     GYEGFERHGLNIVAAFDIDDAKVGRPLRGKEVLPLSKFTDLARRMHIRMGIIATPADA
                     APSIANLMVASGIRAIWNFAPAKIIVPENVVVQNEDLSSGLAVLSRQLAEILEKEGEA
                     "
     gene            5349..>5640
                     /locus_tag="PLY56_05500"
     CDS             5349..>5640
                     /locus_tag="PLY56_05500"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOJ20967.1"
                     /translation="MMELLADHCVGEPTIAVLREAGYRVTRLVDVTGGGDGPTLADGK
                     VAAMAARQGRLLLTEDHDFRTRVHYTHRNHPGVILLRDTATHRDAVHRRLLRL"
BASE COUNT          965 a         1917 c         1769 g          989 t
ORIGIN      
        1 gccgcgctcc tcgatctggt caatcagaga ctgcaggaga cgctgggatc cgcggaccat
       61 gcagctcgtg ccgacgcaga tgttcacctt caccttcgcc tggccgttgc cattcaccag
      121 ggcgatctcc tcgtcgcaga tgcgccggcg gctcgtgtag ttcgtgtgca gcaggtggtg
      181 cgccttctcg ccgcccacct cgccgaggtg ccggtcgtag cactcggtga ccacgtggtt
      241 ctcctgcgac ttgtggaact cgagcatctt gtccgagtcg tagagaccgc gggcgcgcag
      301 gcggctggcg cccggatcgc gagagacagg ctggccagcg ccgccgatgc agccgccggg
      361 gcagctcatc acctcgacga tgtcgtaatc acactcgcct gccatcacgc gctcggccac
      421 ccggcgcgcg ttcgcgagcc cgtggacgat ggccagcttc agatcgacac ccggggcgac
      481 gttcacggtc gcctcgcgca ggccttcctc gccgcgcacg gcgcggaagt cgacgccttc
      541 caggcgagtc ccgctcacct tctccactgc gtagcggagc accgcttcgg tgacaccgcc
      601 ggacgtcccg aagatgacgc ccgcgcccgt cttgaacccg aacggcatat cgagcgattc
      661 aggctccagc cggctgaagc ggaggccctg cgcctcgatc atgcggccca gctcctgggt
      721 cgtcagaacg tagtcgacgt ccggaatgcc atcctggatg aacttctcct gcctcgcctc
      781 gaacttcttc gccgtgcatg gcatgatcga gacgatcacc aggtccttgt tctccacgcc
      841 cagcttctct gggagcacct cgcgggcgat ggagccgaac atctgctggg gcgaccggca
      901 agaggagaga tgcgggagca ggctgggata gtactgctcc gcgaacttca cccacgccgg
      961 gcagcaggag gtgaagatcg gcaagcgctc gcccttctcc ttacgcgcga ggaactcggt
     1021 cgcctcttcg atcaccgtga ggtccgccgc gaaggaggtg tcgtagacct ggtcgaagcc
     1081 catcgccttg agcgcggcca ccgtctggcc catgctcacc gtgccagggg ccagcccgaa
     1141 gtgctcgccg atggcgacgc gcaccgcggg agcgatctgg gccaccacct tcttcttcgg
     1201 gtcgctcagc gcttcccaaa ccgcgtcgat ctccgacttc accgtgagcg cccccgtggg
     1261 gcaaaccgag gcgcactggc cacagtagac gcactcgacg tgcttcaggt ccttgccaaa
     1321 ggcgggcagg accgccgtgg acgcgcctcg gtaggcgaaa tcgatcgcgc caattccctg
     1381 gatctcgtgg cagacgcgca cgcagtcgcc acacaggatg cacttattcg ggtcgcgcac
     1441 aagcgagggc gagctgcggt ccaccggcac cggttggtgg gtggacttga agcgcacctc
     1501 gtcgacgccc aggcgccgag cgatctcctg aagctggcag gaggcgctct tggagcaggt
     1561 ggggcacgtc ttctcgtggt tggccagcag cagctccacg gcgatcttgc ggatctcgcg
     1621 gatctcctgc gtggtcgtct ttaccttcat gcccggttcc gggggcgtgg agcacgagcc
     1681 gacgatcccc cgcccttcca cctccacgag gcagaggcgg caggcaccgt agacgctgag
     1741 gtgagaatgg tagcagaagg tcggaatgtc aatccccgcc ttccggatca cctcgagcag
     1801 gttgcgctcg ccctcgatcc ggacctcttt cccatctatc gttagaaact cgttatcgct
     1861 cattatccgt accattcctg gtcagggcca ccgtcattga acaacgcggc tagagacctg
     1921 tgaccgcccc gaacttgcag ctctcggcgc agacgccaca gcggatgcac ttctcaggat
     1981 cgatggcgtg gggcatcttc ctgtcgccgc tgatggcgcc gaccgggcac ttcttggcgc
     2041 acagcgtgca ccccttgcaa agcgcagggt cgatcgcggg cacgagaagc gccttgcact
     2101 ggccagccgg gcaccgcttc tggacgacgt gcgcctcgta ctcggcgcgg aagtagcgca
     2161 gcgtggagag aaccgggttg ggcgccgtct tgcccaggcc gcagagcgag cccttctgca
     2221 ccgcgcgggc taccttctcg agcaggtcca gcgtctcgag cgtgccctgg ccctcgatga
     2281 tctcctcgag gagggcgagc atctgcttgg tgccctcgcg gcacaggacg cacttgccgc
     2341 acgactcgtt ctgggtgaac tgcatgaaga agtgggcgat cttgaccatg caggtctgtt
     2401 tgttcatgac gacgagaccg ccggaaccga ccatggcgcc cacgctccgc agcgagtcgt
     2461 aatcgagcgg gagatcgagt tgagcctcgg tgaggcagcc gccggaaggc ccgccgatct
     2521 gcaccgcctt gaagccctcg cccgtgatcg cacccgtcgc atcggtgacg ccgccgccga
     2581 tgtggtggac gatctcgcgc aggctggctc cgaaaggcac ctcgatgagg ccggtgttgg
     2641 ccacatgccc ggtgagcgcg aacgtcttcg tgccgggcga cttctccgtg cccatggccc
     2701 ggaactccgc ggcgcccgcg ctcaggatca gcgggatggt cgccagcgtc tccacgttgt
     2761 tgatcaccgt gggctttcca aagagaccgc tctgcgccgg gaaaggcggt ttgggggtcg
     2821 gcatgccgcg cttgccctcg atgctcgaga tgagcgccgt ctcctcaccg cacacgaagg
     2881 cgcccgcgcc ctccatcacc tggatgcgga aggagtggcc cgtgccgaag acgttgtcgc
     2941 ccagaatgcc aagcgcctca gcgtccgcta gcgccttgcg cacgcggcta accgcgagcg
     3001 gatactcggc gcgaacatag atgtagcctt catccgcccc gatggcgcgc gccgcgatca
     3061 tcatcccctc gatgatgctg tgcgggttgc cctccatcaa gctgcgatcc atgaaggcgc
     3121 ccgggtcgcc ctcgtctccg ttgcagatga cgtacttctt gggccccggc tgctggagcg
     3181 tcaggctcca cttcttcccc gtggggaaac cgccgccgcc ccgaccgcgc agtccagagt
     3241 aagtgacctc ctcgcagtcc tcttctggcg tcattcgggt aaaggccgcc tccgcagcgc
     3301 ggtagccccc gttatggatg tactcgcgga tatcctcggg gtcgatggca ccacacgcct
     3361 tgagcacgta gcggtgctga agcgtgtaga aggggatctc gccggtgccg cgacactgct
     3421 tgccgctgag cggatcggtg tagagcaggc gctccaccac ttcgccgccc tgcagggtgg
     3481 tctcgataat ctccgccaca tcctcggggc gaacccgcgt gtagaggatc ccttccggtt
     3541 cgatcgtgac aagcggaccc tgctgacaga agccctggca accgctcttc gagaggcgaa
     3601 ggtcccctcc cgctgtctcg gcacgtagct ccgtggcgac cgataggccg gccgcggcaa
     3661 tctgctgctc gaacgccgca tgaaccttca gcgccccatt ggcgacgcac cccgtgcctg
     3721 cgcagatgat cacccggcgc tcgtaggtgc gcgccgcttg ccagtactcc cgggccacag
     3781 catccaaatc gggccggggc accggctgca tcgtctcgga aacgctcatc gtgactcctc
     3841 gcttgccagg atctcttcaa tcacctcgac tgccttctcg ggcgtcatct gaccatgcac
     3901 ctgctcgttg atcaccacga ccggagccag gccgcacgcg cccaagcaag acaccgtctc
     3961 gaccgtgaaa agcatgtcgg gagtggtgct ctgcttctcc ttcagcccca ggcgctcgcg
     4021 gatcgcctcg aggatcggga tcgatccctt cacgtggcag gccgtgccgt cacagagtcg
     4081 gacgatatac ttccccttcg gctccagcgc gaagtgggcg tagaacgtgg ccaccccata
     4141 gacgcgcgcg ggcgagatgc cgagcgaagt ggcaatgtag gtgagcacat cctccggcag
     4201 gtagcggtac tcctcctgca ccgcctgcag gatgggcacc agccggcccg ggttgcgatc
     4261 gtgctcgtcg atgatctcgc agacgcgctg aaacttgcgg tgctgctgag atgcggagtg
     4321 taaggccaga gccatgtcag gcttcccctt ccttctcgag gatctccgca agctgtcgag
     4381 agagaacagc gagccctgat gagagatcct cattctggac cacaacattc tcgggaacga
     4441 tgatcttcgc tggagcgaag ttccagatag cgcgaatccc agaggcgacc atcaggttgg
     4501 cgatagaggg cgcggcatcg gcgggcgtcg cgatgatccc catgcgaatg tgcatccggc
     4561 gagccaggtc ggtgaacttc gacagcggaa gcacttcctt gccacggagt ggccgaccca
     4621 ccttggcgtc gtcgatgtcg aacgcagcaa cgatgttcag gccgtgccgc tcgaacccct
     4681 catagcccat cagcgctgcg cccaggctgc ccgcgcctac caggaacgcg tccgtcgtgt
     4741 tgtcccaccc caggaactcc tcaatcgcct ggatcaactc cgaaacgggg tagccaaccc
     4801 gacatttccc ctcgatgccc gtcaccgcca ggtccttgcg tacttggatg gactctagat
     4861 ggagctcgtc ggcgatggcg gtactcgata cgaactcgcg accatccttc tccatctgcc
     4921 gcaacaggcg aaggtacgtc ggcagccgcc tcactgtcgg cagcggcgcg cccttctgct
     4981 tcccagtctc catgggtccc cctcctgagt aactggcacc tcgtatggat acgcccttat
     5041 cgataaatca ttatccaatt gcattatcgc atagaagaga gcggttgtca acaccctttt
     5101 gggagaatca cgcgctcgag ggagatccgg gaggcaattc aggcggcgag acagggggct
     5161 acgagggaga gccccgacgg ggctttgccg ggaagcagcg cggagcttcg ggctccgcgc
     5221 atgcgcgcgt ccggcccttt catccgtggt atcggcaccg gcgcgcgggg cgctcgcgcg
     5281 ccgagccagg gtttggaagg gggaataccg atcgcgcgcg aaagagaggg aaggatggac
     5341 acgcggcgat gatggaactg ctggcggatc actgcgtcgg ggagccgacg attgccgtcc
     5401 tgcgagaggc tggctaccgc gtgacgcgcc tggtagacgt caccggaggc ggcgacggcc
     5461 caaccctggc agacggcaag gtcgcggcga tggcggcgcg ccaggggcga ttgctgctca
     5521 ccgaggacca cgacttccgc acgcgggtgc attacaccca ccgcaaccac cccggcgtga
     5581 tcctcctccg agacaccgcc acccaccgcg acgccgtcca ccggcgcctt ctgcggctgc
//
LOCUS       DAOSCV010000166         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9108_length_5614_cov_3.650477, whole genome shotgun sequence.
ACCESSION   DAOSCV010000166 DAOSCV010000000
VERSION     DAOSCV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9108_length_5614_cov_3.650477"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1588)
                     /locus_tag="PLY56_05505"
     CDS             complement(167..1588)
                     /locus_tag="PLY56_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ20968.1"
                     /translation="MQDKAPIIRLLLYSQKLVEAGLKRGEPFHFEEGTPSMFDEFFTL
                     ADELGVFEGIASLEDPRKRAYIPLPVIAATVLCRFLYGLESFRCTGRVLLRNHVLLQR
                     LGVAPEVLEKGGYYQCQRAGDGGDPESAPKLFDEESIADVLARVDVAGLNALLSRFVQ
                     QLRKRQPRWFKRGLFIMDSNHFALKGSRRQYKWCCLMLWTPYGMIPVAAVFSATEGEG
                     TGETSVGRQLLERVFETYGERGFVKLLLMDTGYQDGAMLRWLYDEHGVDWVMDPSEEL
                     IATKGMLAAIDEKPQRPWVRVQPPKLELPKEQMPVRHVMWLGERHNFYTYGRPVNGCV
                     VRDTYPPSEKHPQGHVRYFCLLTSRMDWKGAQIHDTSRMRWCIEDTFGLMTNFWGLGR
                     WEIGRFEVYRATILLMALTFGLLVVYQWEKQLRLPLKRIQERLAVQSQWRVLVVCAGA
                     VVSATPAMLNSWIQKGWLRGVPG"
     gene            1986..2921
                     /locus_tag="PLY56_05510"
     CDS             1986..2921
                     /locus_tag="PLY56_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ20969.1"
                     /translation="MERLAHAWLIEGEIASLSQDTLALRRTVLSKLVWFLQHAGHRSF
                     GLREAKDFIFYLATGHQEPNGRWGNPRFRKPPSPRTVQVYALETRRFLKFLAQEGLID
                     PEAPAQMRMPSVRAHQVQPFTRQQVEALLAAARRTRNARRDEALLLFLLDTGCRASDA
                     CNLLMKDLDLKERSCVVMGKGRKRRAVYFSKHTLRALWNYLREEERDPEDPVFLGERG
                     DPLNRDALRTLMRRLGEAAGLQAVRCSPHTLRHTFAVEFLRAGGDALTLQRLLGHENL
                     AMTNRYVALAQADMANQHRQHSPVEALMRKDGRRT"
     gene            2918..3760
                     /locus_tag="PLY56_05515"
     CDS             2918..3760
                     /locus_tag="PLY56_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20970.1"
                     /translation="MSTFDPAGVARNAGGEFPPSATAGAAAILAYAFLRRIEDLLHAA
                     AGPDILIICEDLDMVYISLLEACRKCLTALGEECPCPAFPEHLPPSLYQWVADGDDMM
                     LWSEYWTLDYEPALRKYLAEMRGYLVKHDLVQNFYLLEQDFEQDDVLGLKHVDRLIRD
                     FEQNASKRRAKGMEALREALQETPENYPVAFPQVAVSGAPTRRASRPGSRLFASEQEF
                     LDACLEAYRNCCAPNPTQETLADKLGCDPRLFRKWQAEAGFSGPEGYSKLLAYLKTRA
                     RPAS"
     gene            3906..4091
                     /locus_tag="PLY56_05520"
     CDS             3906..4091
                     /locus_tag="PLY56_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20971.1"
                     /translation="MPATERLKSRRVPVYLTAEQKLMLARIADLEGEHLAATVRMLLR
                     EGAERRGLVPTVGQEAK"
     gene            4410..4955
                     /locus_tag="PLY56_05525"
     CDS             4410..4955
                     /locus_tag="PLY56_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ20972.1"
                     /translation="MNLDELAAGGTREALEALAADTPDWAPPDAAAPAPLAVVRRLAD
                     VQPEAVMWLWPGWLPRGKLALLDGDPGLGKSLITLDLAARVSRGASMPDGAGGGEPAG
                     VVILTAEDDLSDTVRPRLDAAGADVSKIAALAAVRRRTEGGAEVLETPTLMDLEALRQ
                     AIASVSAALVIIDPLMAYLPG"
     gene            4992..5573
                     /locus_tag="PLY56_05530"
     CDS             4992..5573
                     /locus_tag="PLY56_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20973.1"
                     /translation="MAPLAALAAETNAAVLVVRHLSKTTVGNPLYRGGGSIGIMGAAR
                     AGLIVGKDPEDPTGERRVLAVTKCNLAAPPPGLAYRLAADGPVRVEWLGQTALTADAL
                     LAVPPGGEERTALADAEAFLRAFLADGPVEAKTIQARAREAGIAEPTLRRAKARVGVE
                     AFLTGFGAAGRWHWRLPLRRDDHEIPEMITLKV"
BASE COUNT          946 a         1959 c         1772 g          937 t
ORIGIN      
        1 cgatccacgc ccatcggtgt cggttgtggt tttggcagtg tatctgcggc ggctcttgca
       61 ggccgctttc gcacctatgc ctcgtgctcc cggcgcaagc gcgcctgccc ggcacctgac
      121 catgacaggc gtcgcttccc gggtgttcac gggatcgcgg tcagtctcac ccgggcacgc
      181 cccgcagcca tcccttctgt atccaggagt tgagcatcgc tggtgtggcg ctcaccactg
      241 ctcccgcgca caccaccagc actcgccact gcgactgcac cgccagccgc tcctggatcc
      301 gcttcagcgg cagccgcaac tgcttctccc actggtagac cactaacagc ccaaaggtga
      361 gcgccatcag caagatggtg gcccgataca cctcaaagcg cccgatctcc caccgcccca
      421 acccccagaa gttggtcatc aatccgaacg tgtcctcgat gcaccagcgc atccgcgagg
      481 tgtcgtggat ctgtgcccct ttccaatcca tacggctggt aagcaggcag aagtagcgga
      541 cgtgcccttg cggatgcttc tcgcttggcg gataggtgtc gcgcactacg cagccgttca
      601 ccggacgacc gtaggtgtag aagttatgcc gctctcccag ccacatcacg tgccgcaccg
      661 gcatctgctc tttcggcaac tccagcttcg gcggctgcac ccgcacccag ggccgctgcg
      721 gcttctcgtc gatcgccgca agcatcccct ttgtggcgat tagctcctcc gaagggtcca
      781 tcacccagtc cacgccatgc tcgtcgtaca gccagcgcag catcgctcca tcctggtagc
      841 cggtatccat caacagtagc ttgacaaacc cccgctcgcc gtaggtctca aacacgcgct
      901 ccagcagctg ccggccaacg ctcgtctctc ccgtgccttc tccctctgtc gccgagaaca
      961 ccgccgctac cgggatcatc ccatagggcg tccacagcat cagacagcac cacttatact
     1021 gccggcgtga ccccttcagc gcgaagtggt tgctgtccat gatgaacaac ccacgcttga
     1081 accagcgcgg ctgccgcttc cgtagctgct gcacgaaccg cgagagcagt gcgttcagcc
     1141 ctgccacatc cacccgcgcc agcacatcgg caatgctctc ttcgtcaaag agcttaggcg
     1201 cgctttccgg gtctcccccg tcgccagcgc gctggcactg gtagtagcct cccttttcca
     1261 atacttcagg ggctaccccg agccgctgca gtagcacgtg gttgcgcagc agcactctcc
     1321 cggtgcagcg gaagctctct agcccataca ggaaacggca aagcaccgta gcggcgatta
     1381 ccggcagcgg gatgtaggcc cgcttgcgtg ggtcttcgag cgaggcgatg ccctcaaaca
     1441 cgccgagctc atcggcgagg gtgaagaact cgtcgaacat gctcggggtg ccttcctcga
     1501 agtggaacgg ctcaccccgc ttcaggcccg cttccacgag cttctgcgag tagagcaata
     1561 accgaatgat aggcgccttg tcttgcatgc ggtcatgttc gccacccctg caccgggtac
     1621 ctaccacatc tttgtactat tttacttggc gctcaccacg ccccggccca actacccgct
     1681 cattccttaa cgacaatcga caccgatgat ccacgcccgg cagtagaggg gttgggcact
     1741 tcggcgtcga atccgtgata tacacgcaag aggtcggggg ttcgagtccc tcaccgccca
     1801 ccaggttgcg gcaaaaacct aagaacgacg cgggttcgtg gcaccactgg cgcccttcgg
     1861 aaaggcattc ggagggcact gtgggaccaa acagaccgct tgattcgact cgcactgatc
     1921 ggttgattcg gggccgccgg agggccgcag aagcgccctc gaacggcggc atcccggctg
     1981 cggaagtgga gcggctcgca cacgcctggc tgatcgaagg cgagatcgct tccctctccc
     2041 aggacacgct ggcgctgcgg cgcaccgtcc tgagcaagct cgtgtggttc ctccagcacg
     2101 ccgggcaccg ctccttcggc cttcgggagg caaaggactt catcttctac ctagccaccg
     2161 gccaccagga gccgaacggg cgttggggca acccgcgctt ccggaagccc ccctcgccga
     2221 ggacggtgca ggtttacgcg ctggagaccc ggcgcttcct gaagttcctc gcgcaggaag
     2281 gcctgatcga cccggaggcg cccgcccaga tgcggatgcc gtcggtgcgc gctcaccagg
     2341 ttcagccgtt cacccgccag caagtggagg cgctcctcgc cgctgcgcgc cggacgcgca
     2401 atgcccggcg agacgaagcg ctcctcctct tcctcctcga caccggctgc cgggcgtccg
     2461 atgcctgcaa cctcctgatg aaggacctag acctgaagga gcggtcctgt gtcgtcatgg
     2521 ggaagggcag gaagcgccgg gccgtctact tctccaaaca caccctccgc gccctctgga
     2581 actaccttcg cgaggaggag cgagaccccg aggaccccgt cttcctcggc gaacggggag
     2641 acccgctgaa ccgggatgcg ctccgcaccc tgatgcggcg gctcggggag gcggcgggcc
     2701 tgcaggcggt ccgttgctcc ccgcataccc tgcgtcacac cttcgccgtg gagttccttc
     2761 gcgccggtgg tgatgccctc accctccagc gcctcctcgg ccacgagaac ctcgccatga
     2821 cgaaccggta tgtggcgctg gcgcaggccg atatggcgaa ccagcaccgc cagcactcgc
     2881 cggtcgaggc gctgatgcgc aaggacggga ggcgaacgtg agtaccttcg accccgcagg
     2941 ggtcgcccgg aacgcgggtg gcgagttccc cccctcagcc actgccggag ccgccgcaat
     3001 cctggcgtac gcgttcctgc ggcggatcga ggacttactt cacgcggctg ccggtcctga
     3061 catactcatc atctgcgagg acctcgacat ggtttacatc tccctgttgg aggcatgccg
     3121 caagtgtctg acagccctag gtgaggagtg tccgtgccct gccttccccg agcatctgcc
     3181 accttccctc taccagtggg tggccgacgg cgatgacatg atgctgtgga gcgagtactg
     3241 gacgctcgat tacgaacccg ccctccggaa gtacctggca gagatgcggg gttacctcgt
     3301 gaagcacgac ctggtccaga acttctacct cttggagcag gacttcgagc aggacgacgt
     3361 gctcggcttg aagcacgtgg accggctgat ccgcgatttc gagcagaacg catcgaagcg
     3421 gagagccaag ggtatggagg cactgaggga ggcactccag gagacaccgg agaactaccc
     3481 cgtcgcattc ccccaggttg ccgtttccgg agcccctacg cgccgcgcat cacgcccggg
     3541 cagccgtctg ttcgccagtg aacaggagtt tctggacgcg tgcttagagg cgtacagaaa
     3601 ctgctgcgct ccgaacccca cgcaggagac gttggccgac aagctcgggt gcgacccccg
     3661 cctgttccgg aagtggcagg cggaggccgg cttctcgggg ccggagggct actccaagct
     3721 cctggcctac ctcaaaacga gggcgagacc cgcctcgtag tccggcggca ccgccctctt
     3781 ccggtcctct tccggtcccc ttccggtcct cttccggtcc tgtattctcg cgccccccca
     3841 ggctactata gtatcgtagg cctacgaaac ccctttcgtg ggcctacgga aggagggcaa
     3901 agacgatgcc agcgaccgag aggctcaaaa gccggcgtgt gccggtgtac ctgacggccg
     3961 agcaaaagct tatgctcgcg cggatcgccg acctggaggg cgaacacctc gccgccacgg
     4021 tgcggatgct gctacgcgag ggcgccgagc ggcgcggcct cgtcccgaca gtcgggcagg
     4081 aggcaaagtg aaggtgaatg cggcgcgggc agccagggag gccgcaggga tggacctgca
     4141 ccacgccgcc tggcgcgccg ggcgctcccc ggcgtacctg gccgcgctcg aacgcggcgc
     4201 ggcgccgtgg ccactgcccc tggcccaacg cctcgccgcg atctacggtt gctccctttc
     4261 ttcattcctc cccagacgtt cgggcgccgg cggcgccgcg aacccgccct gccgcaacca
     4321 cagcagaggg gggaagggag aggagcaccg cgcgggtgga acgaggcgat gaaccgcact
     4381 gggctggcgg cgcgaacgga ggcgcgcaaa tgaacttgga cgaacttgcc gccggaggca
     4441 cgcgggaggc gttggaggcg ttggcggcgg acacgcccga ctgggcgcca ccggacgcgg
     4501 cggcgcccgc gccgctcgca gtggtacgcc ggctcgcgga cgttcagccc gaagctgtga
     4561 tgtggctgtg gcccggctgg ctcccgcgcg ggaaactcgc gctgctggac ggggaccccg
     4621 gcctcgggaa atccctcatc actttggatc tcgctgcccg ggtttcgcgg ggcgcgtcga
     4681 tgccggacgg cgccggcggc ggcgaacccg ccggcgtcgt catactgacg gcggaggatg
     4741 atctctccga tacggtccgg ccccggctcg acgcggcggg ggccgacgtg tcgaagatcg
     4801 ccgccctcgc ggcggtgagg cgccgcactg aggggggcgc cgaggttctc gagaccccca
     4861 ccctgatgga tctcgaggcg cttcggcagg cgatcgcgtc cgtctccgcc gccctcgtca
     4921 tcatagatcc cctcatggcg tacctgcccg ggtaggttga tagccaccgc gatcaggatg
     4981 ttcgccgtgt tttggccccc ctggcggcgc tcgccgccga gacgaacgcc gcggtgctcg
     5041 ttgttcgtca cctcagcaaa actactgttg gcaatccgct gtaccggggc ggcggcagca
     5101 tcggcatcat gggggcggcc cgcgccggcc tgatcgtcgg taaggacccc gaggacccca
     5161 caggggagcg gcgcgtcctc gccgtgacga agtgcaacct ggccgccccg ccaccaggcc
     5221 tggcctaccg gctcgccgcc gatggcccgg tccgggtgga gtggttgggg caaaccgcgc
     5281 tgaccgccga cgccctgctc gccgtgccgc cgggcggcga ggagcgaacc gcactggccg
     5341 acgcggaggc gtttttgcgc gccttcctcg ccgacggccc ggtggaggcg aagacgatcc
     5401 aggctcgggc gagggaggcc ggaatcgccg aacccaccct ccggcgggcg aaggcacgcg
     5461 tcggcgttga ggccttcctg accggttttg gggctgcggg tcgctggcac tggcggctgc
     5521 cgctgcgcag agatgatcat gagatcccag agatgatcac cctgaaagta tgatcatctc
     5581 tgggtctgat gatcatctct ggtggaagag gagg
//
LOCUS       DAOSCV010000167         5598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9143_length_5598_cov_3.255097, whole genome shotgun sequence.
ACCESSION   DAOSCV010000167 DAOSCV010000000
VERSION     DAOSCV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9143_length_5598_cov_3.255097"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PLY56_05535"
     CDS             <1..981
                     /locus_tag="PLY56_05535"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOJ20974.1"
                     /translation="GNLPRKLKVAVCGCARHCVPVEINDVGLEAARNEEGTLGFALFV
                     GGGLSASPTLAQPVGWIAQDQAVPVVKAIAAIFREHGNRSNRARARMKHLLGEWGIDR
                     FRAELESRLGWAPRVIPGMALHAGSGRDHLGVLPQKDAGRRLVGVPVAAGLLTADQFL
                     LLAKLAAEHGQGELRTTLYQNILLPDIAEPAVDTVLAALAENGLPVSESRWAGHTIVC
                     TGREFCNKAMAETKKPAIRLLDALEEALPGRPARLRISGCPNGCGQHALGEIGLQGSG
                     VKGESGLEERFDVWVREASPDGAPSFGRRVMSRVRPEEVLAVVREQVARF"
     gene            1205..1666
                     /locus_tag="PLY56_05540"
     CDS             1205..1666
                     /locus_tag="PLY56_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ20975.1"
                     /translation="MKLTTRGQYASRAMLDLAERYHEGAVVIEDIARRQGIPKKYLER
                     LLLDLKGAGLVQSIRGRQGGYRLMRPPQEITLGEVVRAMEGPIAPLACVDEDAPQPCD
                     CPCADVCGLRTVWAEVRDAIAGVLDNTSLETVRQRAEKLREQQAGPAMYYI"
     gene            1962..2900
                     /gene="cysK"
                     /locus_tag="PLY56_05545"
     CDS             1962..2900
                     /gene="cysK"
                     /locus_tag="PLY56_05545"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ20976.1"
                     /translation="MFRSDERIAQDVTELIGGTPLVRLRRVTRGLAAEVVAKLESFNP
                     GGSVKDRIALAMLLKAEEEGRLTPRSVVIEPTSGNTGIGLAMVAAARGYRLILTMPET
                     MSVERRALLRAYGAEIVLTPGAEGMKGAVRRALELAAEIPDSFVPQQFENPANPEVHR
                     RTTAEEIWRDTGGRIDIFVAGVGTGGTLTGVGEVLKARKPDLRVVAVEPADSPVLSGG
                     NPGPHKIQGIGAGFVPAVLNREVIDEILRVENEEAFKIGRRLAREEGILVGISSGAAA
                     FAALKVAARPENAGKRIVVVLPDTGERYLSTALFGE"
     gene            2925..4295
                     /locus_tag="PLY56_05550"
     CDS             2925..4295
                     /locus_tag="PLY56_05550"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HOJ20977.1"
                     /translation="MRYYNSILDAIGRTPLIRLSRVTDGLPVTLLAKVESLNPGGSVK
                     DRIGLAMIEKAERTGLLQPGGTIIEATAGNTGVGLALVAASKGYRCIFVMPDKMSEDK
                     VNLLRAYGAEVVITPTAVPPDSPESYNGVADRLAREIPGAFRPNQFANLANPEAHYRT
                     TGPEIWEDTDGKVDVFVAGMGTGGTISGIGRYLKKRNPKVIVVGADPEGSVLSGDSPR
                     SYKVEGIGEDFVPYTFDRRVVDEMVRVSDAESFRMARRLAREEGLLVGGSSGTAVAAA
                     IAYARRLEPGKVIVALLPDTGRNYLSKIYSDRWMEENGFLEPTQERATAGDVLLSKRG
                     MPGLIAIAPEEPAVEAVLRMERYGISQLPVLAGDQVLGSVNEVTLVKLLHDGVDLAAK
                     TASEVMGKPLPAVDAATDVSEVYRLLLAGHGGVIVSHEGAPIGLLARIDLVNYWTGRG
                     EERRAA"
     gene            4292..5452
                     /locus_tag="PLY56_05555"
     CDS             4292..5452
                     /locus_tag="PLY56_05555"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOJ20978.1"
                     /translation="MKFETRAIHAGQGPDPTTGATIVPIYQTSTFTWDGLGEHRGYEY
                     SRTGNPTRTALESCLASLEEGRYGLAFASGMAAVDAVLSALRPGDHVVAADDLYGGTY
                     RILEKVYRPRQVSVSYVDGRDPEAFAQAIRPETRLAWIETPTNPLLHLVDIAAVAKIC
                     HARGVRVAVDNTFATPYLQRPLALGADIVVHSTTKYINGHSDVVGGAVVTSDPEIYAA
                     AKFYQNAAGGTPSPFDVWLTLRGVKTLAVRMRQHCSNASRVAQYLAEHPRVECVHYPG
                     LPSHPQHALAKRQMADFGGMVTLRLPGGRSEADLFLKSLRVFSLAESLGGVESLACYP
                     VEMTHGAIPEEERVRRGITAGTIRLSVGIEDVDDLLADLGHALAAVGAGSAR"
BASE COUNT          964 a         1748 c         1961 g          925 t
ORIGIN      
        1 gggaatctac cgcgcaagtt gaaggtggcc gtgtgtggct gcgcgcgcca ttgcgtgccg
       61 gtcgagatca acgatgtcgg actggaagcg gcgcgcaatg aagaggggac ccttggcttc
      121 gccctcttcg tcggtggagg cctctccgcg tctcccacgc tggcccagcc ggttggttgg
      181 attgcgcagg accaggcggt tcccgtggtg aaggcgatcg ccgctatctt ccgggagcat
      241 ggcaatcggt cgaatcgggc gcgggcgcgc atgaagcacc tcctgggcga gtggggtatc
      301 gaccgcttcc gcgctgagct ggagtcgcgg cttggctggg ccccgcgcgt catccccggc
      361 atggcgctgc acgctggatc gggccgggac cacctcggcg tgctccctca gaaggatgcg
      421 ggccggcgtc tcgtcggcgt tcctgtcgcc gcggggctcc tcaccgccga tcaattcctc
      481 ctgctggcga agctggccgc ggagcatggt cagggtgagc ttcgcaccac cctctaccag
      541 aatatcctct tgcccgatat cgctgagccg gcggtggaca cggtgcttgc ggcgctcgcg
      601 gaaaacggcc tcccggtctc ggagagccgc tgggccggcc acaccatcgt ctgcacgggg
      661 cgtgagttct gcaacaaggc gatggccgag acgaagaaac ccgccatccg gctgctggat
      721 gcgctggaag aggcgcttcc cggaagaccg gctcgtctcc ggatcagcgg gtgcccgaac
      781 gggtgtggcc aacatgctct gggagagatc ggtctccagg gaagcggcgt gaagggcgag
      841 tccggcctgg aagagcggtt cgatgtgtgg gtgcgcgagg catcaccgga cggcgcgccc
      901 tccttcggcc ggcgtgtgat gtcgcgggtg cgccccgaag aggtgttggc ggtcgtccga
      961 gagcaggtcg cccgcttctg agcggccggc gcatcctgga ccgcgtccgc cccaggcgct
     1021 ggccaggacc atcaccggcc ttctggcgcg tcatcgggcg gaggcactcc cgggcgaggc
     1081 tttctctgcc tacgcccggc gcgtcctgtg aaggaatcgg aggcataggg cggttgacag
     1141 catggcggcg gtgggctata ctaggtaatg tcgattgaaa taatagacat taccggaggt
     1201 tggcatgaaa ctgaccacgc gcggccagta tgcgagccgc gccatgctgg atctggcgga
     1261 gcgctaccac gagggtgccg tcgtgatcga ggatatcgcg cgccggcagg ggatccctaa
     1321 gaagtacctg gagcgcctgc tcctggatct gaagggggcg ggcctcgtcc agagcatccg
     1381 cggccggcag ggagggtatc gcctcatgcg cccgccccag gagatcaccc tgggagaggt
     1441 ggtgcgggcg atggagggcc ctatcgcgcc gctcgcgtgc gtggacgagg atgcccccca
     1501 gccctgcgat tgcccctgtg ccgatgtctg cggcctgcgt accgtgtggg ccgaggtgcg
     1561 agacgccatc gccggcgttc tcgacaacac cagcctggag accgtgcggc agcgtgccga
     1621 gaagctgcgc gagcagcagg ccgggccggc aatgtactac atctgacacc agcctaaaac
     1681 gcacttcccc ccctgccaca ctccctgtgg cgagcgcctc ccctgcctcc cagcgaatac
     1741 ctctgccaca aaccccttga caagttcccg gaggagttga tactatacta ccacgatgga
     1801 cttagtagga taaagcccgg gaatcccgag cggcgccgca cagtagtcga ggcatcgcca
     1861 gaggcggcgc ggtcgttgag agaaactggg gcggcgatgg ccttgacgag ggctggcagc
     1921 ccccctatgg ttcgtcagaa caaggagaga aagggaacgc ggtgttcaga tctgacgaga
     1981 gaatcgccca ggatgtgacg gagctgatcg gcggtacacc tctggttcgt ctgcgccggg
     2041 tcacccgggg gctcgcggca gaagtggtgg cgaagctgga gagcttcaac ccggggggga
     2101 gtgtcaagga tcgcatcgcc ctggcgatgc tgctgaaagc ggaagaggag gggcggctca
     2161 cgccgcggag cgtggtcatc gagccgacga gcggcaacac gggcatcggt ctggcaatgg
     2221 ttgctgccgc gcggggctat cgtctcatct tgacgatgcc ggagacgatg agcgtggagc
     2281 ggcgagcgct gttgagggcc tacggcgccg agatcgtgct cacgcctggt gccgagggga
     2341 tgaagggcgc tgtgcgccgc gcgctggagc tggcggctga gatcccggac tcctttgtgc
     2401 cgcagcagtt cgagaatccg gccaaccccg aggtgcaccg gcggacgact gccgaggaga
     2461 tctggcggga taccggcggc cgcatagaca tcttcgtggc cggggtcggc accggaggca
     2521 cgctcaccgg ggtgggcgag gtcctaaagg cgcgcaagcc agacctgcgc gtagttgccg
     2581 tcgagcctgc cgactccccc gtcctctcgg gcgggaaccc cgggccgcat aagatccagg
     2641 gcatcggcgc cgggttcgtg cccgcggtcc tcaaccgaga ggtcattgac gagatcctgc
     2701 gcgtggagaa cgaagaggcg ttcaagatag gtcgccgcct ggcacgcgag gagggaatcc
     2761 tggtcgggat ctcctcgggt gcagcggcct ttgcggcgct caaggtcgcg gcgcggccag
     2821 agaacgccgg caaacgcatc gtcgtggtgc tccccgatac cggcgagcgc tacctcagta
     2881 ccgcgctctt tggcgaataa cgaacccagg aacgcgagcg gaagatgaga tactacaaca
     2941 gcattctgga cgccatcggg aggactccgc tcatccgcct atcgagggtt accgacggcc
     3001 tgccggtgac cctgctggcc aaggtggagt cactcaaccc gggcgggagt gtgaaggacc
     3061 gcatcggcct cgccatgatc gagaaagccg agcgaaccgg ccttctgcag ccgggcggga
     3121 ccatcatcga ggcgacagcc ggcaacaccg gcgtcggctt ggcgctggtg gcagccagca
     3181 aaggctaccg gtgcatcttc gtgatgcccg ataagatgag cgaggacaag gtgaatctcc
     3241 tccgtgccta cggcgcagag gtggtgatca cccctaccgc cgttcctccc gactcgccgg
     3301 aaagctacaa cggcgtggcc gaccgtctgg cccgggagat ccccggagcg ttccgaccga
     3361 accagttcgc gaatctggcg aacccggagg cgcactaccg taccaccggc ccggagatct
     3421 gggaggatac ggatggcaag gtggatgtct tcgtcgctgg gatgggcacc ggcggaacca
     3481 tctccgggat cggtcgctac ctgaagaagc gtaacccgaa ggtgatcgtc gtgggcgccg
     3541 acccagaggg ctccgtcctc tccggggatt cgccacgctc ctacaaggtg gaggggattg
     3601 gcgaggattt cgtcccctac acctttgatc ggcgcgtcgt tgatgagatg gtgcgcgtga
     3661 gcgatgccga atcgttccgg atggcgcgac gtctggcccg cgaagagggg cttctggtcg
     3721 gtggctcgtc gggcaccgca gtggccgcgg ccatcgccta cgcaagacgc ctggagccgg
     3781 ggaaggtcat cgtggccctg ctccccgaca ccgggcgcaa ctacctgagc aagatctact
     3841 ccgatcggtg gatggaggag aacggcttcc tggagcccac tcaggagagg gcgacggccg
     3901 gcgacgttct cctcagcaag cgggggatgc cgggcctgat cgcgatcgcg cccgaggaac
     3961 cagccgttga agcggtgctg cgcatggagc gctacgggat ctcacaactc ccggtgctcg
     4021 ctggcgacca ggtgctaggg agtgtgaatg aggtgaccct cgtgaagctc ctgcacgatg
     4081 gcgtggacct ggcggcgaag acggcatccg aggtgatggg caaacccctg ccggcggtgg
     4141 atgcggctac cgatgtgtcg gaggtctacc ggctgctgct ggcaggccat ggtggcgtga
     4201 tcgtcagtca tgagggcgct cctatcggcc tcctggcccg cattgacctc gtgaactact
     4261 ggacggggcg gggcgaagag aggagagcgg catgaagttc gagacacggg cgatccacgc
     4321 cggtcagggg ccggacccga ctacgggcgc caccatcgta cccatctacc agacgtccac
     4381 cttcacctgg gatgggctcg gtgagcaccg tggctatgaa tactcgcgga ccggcaaccc
     4441 gacgcgcacc gccctggaat cctgcctggc atcgttggaa gaaggccgct acggtctggc
     4501 ctttgcgtcc ggcatggccg ccgtggatgc cgtgctctcg gcactgcgcc cgggcgacca
     4561 cgtggtggca gccgatgacc tctacggggg cacctaccgc atcctggaga aggtgtaccg
     4621 cccccggcag gtctcggttt cctacgttga cgggcgcgat cccgaagcct tcgcgcaggc
     4681 catccggccc gaaacccgct tggcatggat cgagactccc accaacccgc tcctccacct
     4741 ggtagacatt gccgcggtgg ccaagatctg ccacgcgcgc ggcgtgcggg tcgccgtgga
     4801 caacacgttt gccactccct acctgcagcg cccgcttgcg ctgggcgccg atatcgtcgt
     4861 tcacagcacg acgaagtaca tcaacggcca ctcggatgtg gtcggcgggg cggtggtcac
     4921 cagcgacccc gagatctacg cggcggcgaa gttctaccag aacgccgctg gtggaacccc
     4981 aagccccttc gatgtgtggc tcacgctccg cggggtgaag actctggccg tccggatgcg
     5041 gcaacactgc tcgaatgcca gccgtgttgc ccagtacctg gccgagcatc cgcgggttga
     5101 gtgcgttcac taccctggcc tgccaagcca tccgcagcac gccctggcga agcggcagat
     5161 ggccgatttc ggcgggatgg tgaccctccg cctgccgggc ggtcggtcgg aagcagatct
     5221 cttcctgaaa agcctgcgtg tcttctcgct ggcggaaagc ctgggcggcg tggagtcgct
     5281 tgcctgctac cctgtggaga tgacacacgg cgccatcccc gaggaggagc gcgtgcggcg
     5341 ggggatcaca gcaggaacga tccggctctc cgttggcatc gaggatgtcg atgacctcct
     5401 ggccgatctg gggcatgcac tcgctgctgt cggtgcgggt tcggccagat agctgatcct
     5461 tgccggcggc ttggctgccg gaggccggag cgggtgagaa tcatggaacc tcccgggttc
     5521 ctggattgtc tctccttatg ggaatgggag cggtagggta tatgataggg accgctccct
     5581 cgaaaccgag tacaacaa
//
LOCUS       DAOSCV010000168         5586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9168_length_5586_cov_3.418731, whole genome shotgun sequence.
ACCESSION   DAOSCV010000168 DAOSCV010000000
VERSION     DAOSCV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9168_length_5586_cov_3.418731"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLY56_05560"
     CDS             complement(<1..479)
                     /locus_tag="PLY56_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20979.1"
                     /translation="MKTTCVSGGGGAGTGQKKLRHNLTRGQVPGKPRRARRAEGAPER
                     ASHLGRHANGTPLAIGEPDAFDRGAIREAQEVLRGAIRVRHALDDLWDTQIHRLRQSL
                     AQGEGEVGHLCKGGGSAAVDPIGDLAPAEPGMAQAREHILDGRRSHADKHGSGRLKLL
                     "
     gene            496..1128
                     /locus_tag="PLY56_05565"
     CDS             496..1128
                     /locus_tag="PLY56_05565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20980.1"
                     /translation="MLNYNALLEAIRLLNTECGVGARNMTISTVGIVPGIERLAQERL
                     QVNLALSLHAADDETRARLMPAGRRYPLDQVFEALRGYINATHRRVTLEYLLLQDVND
                     SEEDADRLARLIRTELQVSTRQGTGRRGLLPVVNLIPYNETEAGFRRPSEATVRRFQA
                     RLKAHGIPVTRRLERGGTVDAACGQLRRAAARRQREGGARPTHRSDSSRG"
     gene            1121..3004
                     /locus_tag="PLY56_05570"
     CDS             1121..3004
                     /locus_tag="PLY56_05570"
                     /inference="COORDINATES: protein motif:HMM:NF023403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20981.1"
                     /translation="MDESHDVLSAEEEAALLATTAPRTLLRLLQAEPALVGKLFAGFR
                     VSADSLKLSPVRARLRHESKSNPEMQRILREAWTESHAELVAAITQWRSAEIPGELPG
                     LVARWGQDVVRLALRLDPRTEVREIPLPEPLPEPAPQAAKPKDKRKPPASPPPQEVET
                     SPAEVESLREQIRALKEELREARKRETHLRTEMEQAQRSAQRWETTARTALDEASDYR
                     RAVERVTRQLEREQRARSDLEARARDAERGERHATAQLDALRRKVEDARQIRAAHEAL
                     CPPAEEWIENARSLIHHGQAQIAANFLSPFLLAHPEADRVREVLAEAHEALGATEMAI
                     TGYCTLARKRLRQGNLGEAALFICRALLCSPDHPEARRCLEEVKRAAAGRQGELPHAV
                     LRHLEKAAQRAPRARELLNAVVTSPHGCEPLCITLDTPVEWPQGKRGFTATPRWVLDA
                     IDTNRGEAVERARKGLSQLRTHRPEVYASVMARLNEHDPSYSRVLSGKTRPIVVDGSN
                     VAWQATEEGERPRLANLLGVRRELRALGFFPIRIFVDAALVYQIDRRAELETLVSRGE
                     ILVADPGTDADEQILDEARALGAAVVTNDRMEDHDPGGRVPKVRFDIEPGGPVVHVRP
                     VRR"
     gene            3197..4057
                     /locus_tag="PLY56_05575"
     CDS             3197..4057
                     /locus_tag="PLY56_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ20982.1"
                     /translation="MQCSKCRAEVNPQAQFCWQCGAPLSPAAPTSPPADQARSKRWTA
                     VAAALALLLLLAGLLLPRLLRTRGPAVTTSPRELPETSAPPVTAAPPANTPQAEALTT
                     RPAGNLPAGSGLPQGQTPPALPFAEDVAAYLRKLEMIQQRDAQNPMAQLFAMMATGLS
                     GLTDALKGLTDETVGETSGNQTSGYLAQFDQLLQAKQALVRDFQAITPVPQPCQQLHT
                     LYGHYLNTQVRAIYDLRNAVARADNLAAPLGVLPVGKQAEAAQQAASQEEKRIRQQYN
                     IPRAAIPLVP"
     gene            4159..5502
                     /locus_tag="PLY56_05580"
     CDS             4159..5502
                     /locus_tag="PLY56_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20983.1"
                     /translation="MRSITSAITRRFSIRMSMACRKGQALVTAMALVVALTILVAATQ
                     HQVVQELKVTKTERDYERALQLAEAGLNAYINRIDYGAAPADVSNPNDPGHLIPPLRE
                     FPIGAAPSLADFRRGVRDGTYTLIRYPPGSQQGYLVGSIGTPGETARFISFGWSNGVV
                     RRVVGVARQASGDYALYGVASVTMHNNQSVTGNVGTNGQVTMHNNNEIENGYLVLNGP
                     GADVSQHKNPGIRVRRGPTPYRWPTVAEIALEKWPNSGATAPGGLEYLATHNDNDLAT
                     IDGVPGIPGNKIDLKNNSTVVFRGKPGGAHYYLTGLTGKNNGTIHLDNSAGPITIWFG
                     PSGGNGEFEMKNGWDLTQAVTDPAHLCRFYVATRGGVTIKNNGASAIGIYAYNVENGQ
                     PFGSVTLKNNSTFTNCRIIANTISVEQNTTITYTPAPYFYPEDNLYAITDWREFE"
BASE COUNT          997 a         1879 c         1858 g          852 t
ORIGIN      
        1 aggagcttga ggcggcccga cccgtgcttg tcggcatgac tacgccggcc atcgaggatg
       61 tgttcgcgcg cctgggccat gcccggttcc gcgggcgcca ggtcgccgat tgggtctacc
      121 gccgcggagc cacctccttt gcagagatga ccgacctccc cctcgccttg cgccaggctc
      181 tgccggaggc ggtggatctg ggtatcccag agatcgtcca gagcgtgtcg gacgcggatg
      241 gcaccacgaa gtacctcttg cgcttcccgg atggcgccac ggtcgaatgc gtccggctcc
      301 cctatcgcga gtggagttcc gtttgcgtgt cgacccaggt gggatgccct ctccggtgca
      361 ccttctgcgc gacgggcgcg tcggggttta cccggaacct gaccgcgggt gagattgtgg
      421 cgcagtttct tctggcccgt gccggcgcca ccccccccgc tgacacacgt ggtcttcatg
      481 ggtatgggag agccgttgtt gaactacaac gcccttctgg aggcgattcg cctcctcaac
      541 accgagtgcg gggtaggcgc tcgaaacatg acgatctcga cggtcggtat cgtgcccggg
      601 atcgagcgcc tggcacagga gaggctgcag gtgaacctgg cgctctccct gcacgccgcc
      661 gatgatgaaa cgcgcgcgcg cttgatgcct gccggccgcc gttaccccct cgaccaggtg
      721 ttcgaggcac tccgaggcta catcaacgcc acgcaccggc gcgtcacctt ggagtatctg
      781 ctactacaag acgtgaacga ctcggaggag gatgccgacc gcctggcccg cctgatccgc
      841 accgaacttc aggtttccac gcgtcagggt accggcaggc gcgggctttt gccagtcgtc
      901 aacctgatcc cctacaacga gacggaagcg ggtttccgcc ggccctcgga agcaacggtg
      961 cgccgcttcc aggcacgcct gaaggcgcat ggcattccgg tgacccgccg tctcgagcgg
     1021 gggggcacag tggacgcggc ctgcgggcag ctgcggcgtg ctgcggcacg ccggcagcga
     1081 gagggcggcg cccggcccac ccacaggagc gattctagcc gtggatgaga gtcacgacgt
     1141 cctctcagcg gaggaggaag cggcgcttct ggcgacgacg gcgccccgga cgctcctccg
     1201 tctgttgcag gccgagccgg cgctcgtggg aaagctcttc gccggcttcc gagtcagcgc
     1261 cgacagcctg aagctctccc ccgtgcgtgc gcggttgcgc cacgagagca agtccaaccc
     1321 ggagatgcag cggatcctgc gcgaagcctg gaccgagagc catgcggagc tcgtcgccgc
     1381 catcacgcag tggagatcgg cggagattcc gggggagctg cctgggttgg tcgcgcgttg
     1441 gggccaggat gtggtgcggc tggcgctccg gctcgacccg cgcacggagg tgcgcgagat
     1501 ccccctcccc gagccactgc cggagccagc gccccaggct gcaaagccca aggacaagcg
     1561 caagcctccg gcttccccgc cgccgcagga ggtggagacc tcaccggcag aggtggagtc
     1621 gctgcgcgag cagatccggg cgctcaagga ggagttgcgc gaggcgcgca agagggagac
     1681 gcacctgcgc accgagatgg agcaggctca gcgatccgcc cagcgctggg agacaaccgc
     1741 ccgcacggcg cttgatgagg cgagcgacta ccggcgcgcg gtggagcgcg tcacccgaca
     1801 gctcgagcgc gagcagcgcg cgcggagtga tctggaggcg cgtgccagag acgccgagcg
     1861 tggcgagcgg catgccacgg cgcaactcga cgcgctgcgc cggaaggtgg aggatgcgcg
     1921 gcagatccgg gccgcgcatg aagccctctg ccctcccgcc gaggagtgga ttgagaacgc
     1981 ccgttccctg atacaccacg ggcaggcgca gatcgccgcg aacttcctgt cgccgttcct
     2041 tctggcacat cccgaggccg atcgggtgcg cgaggtgctg gcggaggcgc atgaggcgct
     2101 gggagccaca gagatggcga tcaccgggta ctgcaccctg gcgcggaagc gcctgcgcca
     2161 gggcaatctc ggtgaagcgg cgctcttcat ctgtcgggcg ctcctctgct cgcctgatca
     2221 ccccgaggcg cgtcgctgcc tcgaggaggt aaaacgcgcg gctgcgggca ggcaggggga
     2281 gctaccgcac gcggtgctcc gccatctcga gaaagcggct cagcgggcgc cacgtgcccg
     2341 cgagctcctc aacgccgtgg tcacctcgcc ccacggatgc gagccgctct gcatcacgct
     2401 cgataccccg gtggagtggc cccagggcaa gcgtggcttc acggcaacgc cgcgctgggt
     2461 tctcgatgcc atcgatacca accgggggga ggcggtggag cgtgcccgaa aggggctgag
     2521 ccagctgcgc acgcacaggc cggaggtgta cgcgtccgtg atggcgaggc tcaacgagca
     2581 tgatccctcc tactcgcgtg tgctctcggg gaagacgcgt cccatcgtcg tggatggaag
     2641 caacgtggcc tggcaggcga ccgaagaggg ggagcggccc cggctggcga acttgcttgg
     2701 tgtgcgaagg gagttgcgcg cgcttggctt cttccctatc cgcatctttg ttgacgctgc
     2761 gctcgtctac cagatagacc gccgggcgga attggagacg ctcgtcagcc ggggggagat
     2821 cctcgtcgcc gatccgggca ccgatgccga cgagcagatc ctggatgagg cccgcgcctt
     2881 gggggccgcc gtggtcacca acgatcgcat ggaggatcac gatccgggag ggcgcgtgcc
     2941 gaaagtgcgc tttgacatcg agccgggcgg gccggtggtg catgtcaggc cggtgcgccg
     3001 ctgaagccgt tcaaacgaaa gggcccgccg gaaggacggg ccctgttagg gcgaactgtt
     3061 tggattggag gtcagcgacc tcgaggggtg cctaaccccc acagtcacct cgactatcac
     3121 tttgcattat agtcatcacc tcctcgattc ggattatacc accggcatct ggaggtgtca
     3181 aggacggagg aagaagatgc agtgctcaaa atgcagggcc gaagtcaacc cgcaagcgca
     3241 gttctgctgg cagtgtggtg ccccgctttc accggcagcg ccgacctcac ctcccgcgga
     3301 tcaggcgcgg agcaagcggt ggacggcggt cgccgctgca ttggcgctcc tcctcctgct
     3361 ggccgggctc ctgcttcctc gattgctgcg aacgcgcggt ccggccgtca ccacctcccc
     3421 gcgggagctt ccggagacat ccgccccacc agtgaccgcc gcgcctcccg cgaacacgcc
     3481 tcaggccgag gcgctcacca cccgacccgc cggcaacctg ccggccggct cggggctccc
     3541 gcaaggccag acgccgcccg ccctgccctt cgccgaggat gtggcggcct acctgcgcaa
     3601 gctcgagatg atccagcagc gcgatgccca gaacccaatg gcccagctct tcgcgatgat
     3661 ggccaccggc ctgagcggcc tcacggatgc cctgaagggc ctcacggatg agactgtcgg
     3721 cgagacatca gggaaccaga ccagtggcta cctggcgcag ttcgatcaac tgctccaggc
     3781 caagcaggcg ctggtccgcg acttccaggc gatcaccccg gtaccccaac cgtgccagca
     3841 gttgcacacg ctgtatggcc actacctgaa tacgcaggtg cgcgccatct acgacttgcg
     3901 gaacgccgtg gcacgggcag acaacctagc ggcgccgctg ggggtgctgc ccgtaggcaa
     3961 gcaggcggag gcggcgcaac aggcggcgag ccaggaagaa aagaggatcc gccagcagta
     4021 caacatcccg cgggccgcca ttccgctggt gccgtaaagc caaggggaca gtcgctctgg
     4081 cctggcgtgc aagccctcag tttggcatgg aggatgctgt aagcgcttac agttgctccc
     4141 ggacccagga ggagcaccat gcgcagcatc acttcggcca tcacgcggag attctccatc
     4201 cgcatgagca tggcatgccg caaaggccag gcgctcgtca ccgcgatggc gctcgtcgtt
     4261 gcgctcacta tcctggtcgc tgccacccaa caccaggtcg tccaggagct gaaggtgacc
     4321 aaaacggagc gcgattacga gcgcgccctg cagttggcgg aggctggtct caacgcttat
     4381 atcaaccgga tcgactatgg cgcggctccg gcggacgtga gcaatccgaa tgacccgggg
     4441 catctcatcc cgccgctccg tgagttccca atcggtgcgg ctccctcgct ggctgatttc
     4501 cgacgaggcg ttcgcgatgg gacctacacg ctgattcgct atccccctgg ctcacagcag
     4561 ggctacttgg tcgggagcat cggcacccca ggggagaccg cccggttcat ctcctttggc
     4621 tggtccaacg gagtggtccg acgcgttgtc ggcgtggccc gccaggcctc gggagactat
     4681 gccctctacg gggtcgcctc ggtcacgatg cacaacaacc aaagcgtcac cgggaatgtg
     4741 ggcaccaacg gccaggtgac gatgcacaac aacaatgaga tcgagaacgg atacctggtg
     4801 ctcaacgggc ccggggccga cgtgagccag cacaagaacc caggcattcg ggtgcggcga
     4861 gggccaacgc cgtatcgctg gccgacggtt gccgagatcg ccctcgagaa gtggccgaat
     4921 agcggcgcca ccgcgcccgg cggcctggaa tacctggcca cgcataacga caacgacctg
     4981 gcgaccatcg atggcgtgcc cggcattcca ggcaacaaga tcgacctgaa gaacaactcc
     5041 acggtcgtct tccgcggcaa gccgggcggc gcccactact acctgacggg tctcaccggc
     5101 aagaacaacg gcacgattca cctggacaac tccgcgggcc cgatcaccat ctggtttggg
     5161 ccgagcggtg gcaacgggga gttcgagatg aagaacggct gggatctcac ccaggcggtc
     5221 accgacccag cccacctctg ccgcttctac gtcgccaccc gcggcggggt gacgatcaag
     5281 aacaacgggg cttcggccat cgggatctac gcgtacaacg tggagaacgg gcagccgttc
     5341 ggcagcgtca cgctcaagaa caactcgacg ttcacgaact gccggatcat cgcgaacacg
     5401 atctcggtgg agcagaacac caccatcacc tacacccccg cgccctactt ctacccggag
     5461 gataacctct acgcgatcac cgactggcga gaattcgagt agccgtcacg gccggatggc
     5521 cctggagggg ccggcccgag aaagcccggc ccctccatcg gaaggctaga tcgcctcgcc
     5581 ttcgac
//
LOCUS       DAOSCV010000169         5581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9181_length_5581_cov_3.388527, whole genome shotgun sequence.
ACCESSION   DAOSCV010000169 DAOSCV010000000
VERSION     DAOSCV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9181_length_5581_cov_3.388527"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLY56_05585"
     CDS             complement(<1..679)
                     /locus_tag="PLY56_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ20984.1"
                     /translation="MARKRGPWKQGAAISGTRSHRIWVALLLLTALLPGQWGCHTAAD
                     PPEDAPAQNGAPAAPSPQMPEDEFDGERSMAWLRKQVDAGFRVPGTKTHATVRRMLVE
                     GLGPTAQVQEFTHQTRQGHLPLANIIATYGPEEGDRVLLCAHWDTRPFADQDPNPANR
                     NQPIPGANDGASGVAVLMELSRLFRAAPPPVGVTIVLFDGEDLGRGLDEMFLGSRYFA
                     EHPIGGPF"
     gene            complement(915..2213)
                     /gene="traF"
                     /locus_tag="PLY56_05590"
     CDS             complement(915..2213)
                     /gene="traF"
                     /locus_tag="PLY56_05590"
                     /inference="COORDINATES: protein motif:HMM:NF025106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HOJ20985.1"
                     /translation="MKRFLRWCTVGLVAAALAYSAPARADLSFGYEARYVGLGGAGLA
                     VIDNPTQAGLINPAALSLRPAAFGMQWPNIGLRWRGAGIGDAFDQLASGSLSVTEAVD
                     LARELGTKPTRVEANLGFGFALSSLDVRARALGNVAVRPSDLFTSWAISGQEPGAWLQ
                     NSSAASQLRAEFNGVTTVDEANAALNRVGDQLGAHLVGAATVSPSIGFGMKVPNRLLG
                     GSRIGDVRVGVRVKPTRVYYSHWVVKPSAQLSQEDVNPNGGLSDSAWGKIKLQGQAAS
                     ELGGKDSLEETSFGMDFGLLWQPAYIPYTTIALTVDNLIEPSFRIPAGADASIVPGRG
                     DKLMPRTFNLGAAMTIPGGLLLAADLLDITGANSANPNGEAQFRIGAEFRPSLPILKW
                     FAIRAGYNSATGMAAGLSLGNFGIAYAERSPLVASQTFNF"
     gene            2639..4138
                     /locus_tag="PLY56_05595"
     CDS             2639..4138
                     /locus_tag="PLY56_05595"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOJ20986.1"
                     /translation="MKIRVMPLGAEVEAAPGQALQDVLFSLGVEFPCGGRGRCKGCRI
                     QVLEGSLSPRPADYEAFTPEELRDGWRLSCRARVEGDLVIQVRQWDTLVLYDNTPVEF
                     EPQEGLGIAIDIGTTTVVAQLLDLATGQVRAVRAGLNPQARWGADVISRMEHAMAGEA
                     AALSDAINQGLSAMVLALFESTRLPWTELRKIAVVGNTVMQHLMLGFPLEQLAMAPHV
                     PYTMEEQTRSTAELGWHLPVDVPLRFLPNLGGFVGSDILGVILATRILESDVLVAAAD
                     LGTNGEIVAGSREGVLVSSTAAGPAFEGGRIEMGMRAGKGAISRVSVVDGRMEVQVIG
                     GGAPRGICGSGLVDAVAAGLELGLIQPTGRITEGDRVVIQEPVAITQRDVRELQLAKA
                     AIAAGLRMLLEELGKTEEDVQKFYLAGAFGNTLGVTSAEQIGLFRFGSERTIPVGNAA
                     LRGAKIALLSREGGVPAGIQERIRFLNLSARPRFQELFAEKMLLDPGPM"
     gene            4196..4381
                     /locus_tag="PLY56_05600"
     CDS             4196..4381
                     /locus_tag="PLY56_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20987.1"
                     /translation="MADRVARKRARICFLLIAAVVAAWAPHLLPVADRWLEARRIRTN
                     IAILYTTDTRGFLDSCG"
     gene            4505..5545
                     /locus_tag="PLY56_05605"
     CDS             4505..5545
                     /locus_tag="PLY56_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOJ20988.1"
                     /translation="MILRIMGADRYDAVGVGALDLQYAREYAQAAPGQIPLVRALPRD
                     DALARLSVDLRVKRLRGRDIGVTSLPPPPEGTGREAYLDAAARALAEMRQRCDFLVLL
                     SQLGLEGDLELARRCGSAGPHAIIGNMEAATLREPLHVGDTVLLPTGAGGTHVGIAEI
                     VFPAWGRPRIEHVAVVPAKVEGPNRAADPAIEDTIARFYESEQDAFVARIDRRLLAAE
                     MAEGAGDEQRCGSCHRHAVATWRETAHSRAVDTLIERKRLVPECLTCHSTRYAATGRF
                     DHAARAGDVDCTGCHPVVSEKESACPEKAARTRGVASCASCHTPAHSPGFNYTSYLSR
                     VNHAWKPAGIPR"
BASE COUNT          903 a         1785 c         1977 g          916 t
ORIGIN      
        1 ggaagggacc gccaatcggg tgctccgcga agtagcgcga gcccaggaac atctcatcca
       61 ggccacgccc caagtcctcg ccgtcaaaga gcacgatcgt cacgcccacc ggtggcgggg
      121 cggcacggaa gaggcgcgag agctccatca agacggccac tcccgaggcc ccatcgttcg
      181 cacccgggat cggctggttg cgattggcgg gattcgggtc ttgatcggca aacgggcgcg
      241 tgtcccaatg cgcgcagagg aggacgcggt cgccctcttc cggcccgtag gtggcgatga
      301 tgttggccaa agggagatga ccctggcgcg tctggtgggt gaactcctgg acctgcgcgg
      361 tggggcccaa gccctcgacc agcatgcggc gtaccgtggc gtgcgtcttg gtgccgggca
      421 cacggaaccc ggcatccact tgcttgcgca accacgccat gctgcgctcg ccgtcaaact
      481 catcctcggg catctggggg gagggcgcgg cgggcgcccc gttctgagcg ggcgcatcct
      541 ccggagggtc cgcggcggtg tggcatcccc attggccggg aagcagggcg gtcagcagca
      601 gcagcgccac ccatatccga tggctccgcg tgccactgat tgcggctccc tgcttccatg
      661 ggccacgctt tcgtgccatg ctcttttcct tcattctcgc cgtcactccc cgctacttct
      721 aggctccatc gcggaaatgc ccttcgccgc tcgaaaacca ccgatgagga cgcggtgggg
      781 gggcgtctgc ccgcagcatc gagtgcattg tggcgtcgca tagtacgcga cgctcagaaa
      841 tgacgcccgg ctggggcacc ggcgtgatgg cggagggagc gccgggcgtc cggcgctccc
      901 aaacccacgc aaccctagaa gttgaacgtc tggctggcca cgagcggcga gcgctcggcg
      961 taggcgatgc cgaagttacc gagtgagagg ccagcggcca tgccggtggc gctgttgtag
     1021 ccagctcgga tagcgaacca cttgagaatg ggcaggctcg gacggaactc tgcgccgatt
     1081 cggaactgcg cctcgccgtt gggattggcg ctgttggcac cggtgatatc cagcaggtcg
     1141 gcagccagga gcagcccgcc cggtatggtc attgccgcgc cgaggttgaa ggtgcgcggc
     1201 atcagcttgt cgccgcgccc cggaacgatg ctggcgtccg cgcccgcggg aatccgaaag
     1261 ctgggctcga tcaggttatc gaccgtgagg gcaatcgtgg tataggggat gtaggccggc
     1321 tgccacagaa ggccgaaatc catgccgaag ctggtctctt ccaggctgtc tttgcctccc
     1381 agttcgctgg ccgcttgccc ctggagcttg atcttgcccc atgcgctgtc gctcagaccg
     1441 ccattcgggt tgacatcctc ctgcgacaac tgggcgctcg gcttgaccac ccagtggcta
     1501 tagtaaactc gggtgggctt gacacggacg cccacacgca cgtcaccgat gcgagagccg
     1561 ccaaggagcc tgttgggcac cttcatgccg aagccgatgg agggcgagac ggtggcggcg
     1621 ccaacgagat gcgcgcccaa ctgatcgcca acgcggttga gcgcggcgtt cgcttcgtca
     1681 acggtggtca cgccgttgaa ctcggctctc agctggctgg cggcagagga gttctgcagc
     1741 caagcccccg gctcttgccc ggagatcgcc caagaggtga acagatcgct cggccgtact
     1801 gcgacgttac caagggcgcg tgcgcgcaca tcgagcgagc tgagagcgaa gccgaagccc
     1861 aaattggcct cgacgcgcgt tggctttgtg cccagctccc gggccagatc cacggcttcg
     1921 gtcacgctca gcgagccgga cgcgagctgg tcgaatgcat cgccaatgcc ggcgccgcgc
     1981 cagcgcaggc cgatgttagg ccactgcatc ccgaacgcgg cggggcggag cgagagtgcc
     2041 gccggattga tgagcccggc ctgcgtgggg ttgtcgatca ccgccaggcc ggcgccgccg
     2101 aggccgacgt agcgcgcctc gtagccgaag ctgagatcgg cacgggctgg ggccgagtaa
     2161 gcgagagctg cggcgaccaa tcctacggta caccatctga ggaaacgctt catgtggtaa
     2221 atcctcccct ctgggggtag cactgcctcg tttgcgagcg ctgaacgcgg gcatcccttg
     2281 tgggccgacg aagtggccgg ccccatggat ggcgcctgtg cccaacgccc gccgggcccc
     2341 ttgtcggtcg cgcgtgaaat gcccggccct gcgaggatgt tgccgggtag cgcgccacca
     2401 cagcaccgcg cgaagagtgg cgagctgtct cgaggcggaa cacgcggtgt gctcaggtcg
     2461 ccaccgcgtg tctgctacgc ggccagtata gcacgcaccc gcgccagtgt caacgggcat
     2521 gcgcggaagc agcgccgacg cgcccggcgg cgcagaacgc aggactttca ccgcggcggg
     2581 gcgaagagtg acacgacaga ggatttcctc cctgtccaac ggagccaggg gattcctgat
     2641 gaagatacgc gtgatgcccc tcggggctga agtcgaggcc gcgccgggtc aggcgctgca
     2701 ggacgtgctc ttttcgctgg gcgtggagtt tccgtgcggc ggccgtggcc ggtgcaaagg
     2761 atgccggatc caggtgctgg agggctctct ctcaccacgg ccggccgatt acgaggcgtt
     2821 cacccccgag gagttgcgcg atggctggcg gctcagctgc cgggcgcgag tagagggaga
     2881 tctggtgatc caggtccgcc agtgggatac cctggtgctc tacgacaaca ccccggtgga
     2941 gtttgagccg caagagggcc tcgggatcgc gatcgatatc ggcaccacga ccgtggtggc
     3001 gcagttgctg gacctggcaa caggccaggt gcgcgccgtg cgcgcgggcc tgaatcccca
     3061 ggcgcgctgg ggcgctgacg tcatctcgcg catggagcac gcgatggcgg gcgaggccgc
     3121 ggcgctgagc gatgcgatca accagggtct atcggccatg gtgcttgccc tgttcgagag
     3181 cacgcgcctg ccgtggaccg agctccgaaa gatcgccgtc gtgggcaaca cggtgatgca
     3241 gcacctgatg ctcggcttcc cgctcgagca gctcgcgatg gcgccgcatg tgccctacac
     3301 gatggaggag cagacgcgga gcacggcgga gctgggctgg cacctccccg tggacgtgcc
     3361 gctacgcttc ctgcccaacc tgggcggatt cgtgggcagc gatatcctcg gcgtcatcct
     3421 ggcgacgcgc atcctggaga gcgacgtcct ggttgcagcc gccgatctgg ggaccaacgg
     3481 cgagatcgtg gcaggcagcc gcgaaggggt tctcgtctct tcgacggcgg ccggtccggc
     3541 gttcgagggg ggacgcatcg agatggggat gcgcgcgggc aagggcgcca tctcacgcgt
     3601 gagcgtcgtg gacggccgga tggaggtcca ggtgatcggt gggggagcgc cccgcggcat
     3661 ctgtggcagc ggtctggtcg atgccgtcgc cgccggcctg gagctcggcc tgatccagcc
     3721 caccgggcga atcaccgagg gggatcgcgt ggtgatccag gagccggtcg ccattaccca
     3781 gcgagacgtg cgcgagctgc aacttgctaa ggccgcgatt gccgccggct tgcgcatgct
     3841 cctcgaagaa ctgggtaaga cggaggagga tgtccagaag ttctacctgg cgggggcgtt
     3901 cggcaatacc ctgggcgtga cgagcgcgga gcagatcggt ctcttccgat tcgggagcga
     3961 gaggacgatc ccggtgggga atgctgccct gcgtggtgcg aagatcgcgc tcctctctcg
     4021 cgagggcggg gtgccggcgg ggatccagga gcgaatccgc ttcctcaatc tctctgcccg
     4081 accgcgcttc caggagctgt tcgccgagaa gatgctgcta gaccccgggc cgatgtaggc
     4141 ggtcggcccg tgggcgccgc cgatttcctt tacgccttga gaggaggtga tcccgttggc
     4201 cgatcgagtg gcgcgcaagc gcgcgaggat ctgctttctc ctgattgcag ccgtggtagc
     4261 cgcgtgggcg ccgcacctcc tcccggtggc ggaccggtgg ctggaggcgc gccggatacg
     4321 cacgaacatt gccatcctct atacgactga cacgcgcggc ttcctcgata gctgtgggtg
     4381 agggggtgcc ctgggggccg gtctggctca ccgtcacacc ttcttagccc gcgagcgcga
     4441 acgtcgtccc ttctcgctgc tggtcgatgg cggtaacgcg gcggatactc cgacgaagct
     4501 ggaggtgatc ctccgtatca tgggggccga ccgctacgac gcggtgggcg tgggtgcgct
     4561 cgacctgcaa tacgcgcgtg agtatgccca ggcagcacct ggccagattc ccctggtgcg
     4621 ggcgcttccc cgcgacgatg cgcttgcccg cctgagcgtt gacctgcgcg tgaagcggct
     4681 gcgaggccgc gatatcggag tgacttcgct gcccccgcct cccgagggga ctgggcgcga
     4741 agcctacctc gacgcggcgg cgcgtgccct tgccgagatg cgccagcgct gcgacttcct
     4801 ggtcctgctc agtcaacttg gtctagaagg cgatctggag ctcgcgcggc ggtgtggaag
     4861 cgcggggccg cacgcgatca tcggcaatat ggaagcggcc accctccgcg agccgctcca
     4921 tgtgggcgat accgttctgc tacccacggg agcgggcgga acccacgtgg ggatcgccga
     4981 gatcgtcttc ccggcatggg gccggccccg gatcgagcat gtggccgtgg tgccggcgaa
     5041 ggtggagggg ccgaaccgcg ccgccgatcc ggccatcgag gatacgattg ctcgcttcta
     5101 tgaaagcgag caggacgcgt tcgtggcgcg aatcgaccgc cggctactcg cagcggagat
     5161 ggcagagggc gcgggcgatg aacagcgttg cggcagctgc caccggcacg ccgtcgcgac
     5221 gtggagagag acggcgcact cccgggcggt cgataccctg atcgagcgca agcgtctcgt
     5281 ccccgagtgc cttacctgcc attcgacgcg ctatgccgcg acgggccggt tcgatcatgc
     5341 ggcgcgtgcc ggcgatgtcg attgcaccgg gtgtcacccc gttgtctcgg agaaggaatc
     5401 tgcgtgcccg gaaaaggcgg cgcgcacgcg gggagtggcg tcgtgcgcct cgtgtcacac
     5461 cccggcgcat agccctgggt tcaattacac gagttacctg tcgcgtgtca accatgcctg
     5521 gaagccggct gggattccga gataaggaat ccgaagccac ggaggcagca cgcaacccgg
     5581 c
//
LOCUS       DAOSCV010000170         5577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9184_length_5577_cov_3.989859, whole genome shotgun sequence.
ACCESSION   DAOSCV010000170 DAOSCV010000000
VERSION     DAOSCV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9184_length_5577_cov_3.989859"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..977
                     /locus_tag="PLY56_05610"
     CDS             270..977
                     /locus_tag="PLY56_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20989.1"
                     /translation="MATRMRKRGFTLIELLVVIAIIAILAAILFPVFARARENARKST
                     CQSNLKQIMLGVLQYTQDYDECMPIYRWNHAAVPSVWVDRDNSAANDRHFWLEAIQPY
                     LKNTQMLFCPSQSFGPTTLSNGKLYVAYAYNGAMSGKSLALFQSTADKICIGDIGERH
                     ADRTPTGYVENWFINSDGTTSWKQWSWWCSIHLGGGNYGYLDGHVKFHTENDKVLGPV
                     LATGSSLPGDQTGYWMP"
     gene            1333..2235
                     /locus_tag="PLY56_05615"
     CDS             1333..2235
                     /locus_tag="PLY56_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20990.1"
                     /translation="MTAKDHMRGLVELPEGDLQAAERMVRGVKQYAAGAETAPLEAFV
                     RQATPGEALPLFDAQAGFGGASKGDPTTVSADDCLRAMAKVRVHRALVRTEPDTLAVD
                     PLLANDLLLEACEVSDSLVPCPIVLPATGGDVPPEADQVDHLIRCGAGAAMVRPAQDD
                     WQWLPFVYTPLMNALSERRLPLFCIERHVSLGQVADVAARFPDLPIIVANVGYRAQRT
                     LVPLLATYRNVHLSLGSNWTVHRGIEQMVEWLGPEQLLFGTGFPAAEPMMAITQLMYA
                     EISEEARALIGSGNLERLWSGVKR"
     gene            2232..3017
                     /locus_tag="PLY56_05620"
     CDS             2232..3017
                     /locus_tag="PLY56_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ20991.1"
                     /translation="MKTLLERARSGESLADLEIVDMHGHFGRYLHAIPDLTAEGLVRV
                     MDRLGVRSILVSHMQCMSQHTHRGNEEVLTAMRAFPGRILGYAVVWPVDGDTRAEMEW
                     CAAHGFTGLKLHSSNGFAYTDPVYAGALSVANERRMPVLLHAWGAASDFAAIRELAAK
                     YPEASFLFAHSGTGGAEAECIRLAREVPNAYLDLAYSQGPRGLVERLVAGAGAEKVLF
                     GSDCYFFSMTQQIGKVLGADLSDGEKRKILSENALRILERVQR"
     gene            3172..4881
                     /locus_tag="PLY56_05625"
     CDS             3172..4881
                     /locus_tag="PLY56_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOJ20992.1"
                     /translation="MTVRYSYRAGLMAAILLLGGAGQTALLAGQAAGAARVSGEQEGV
                     LQVKDGKLPAGPAEIRQGGAVVGFARVDPEGVARRVLGQAPRDAEIHPIAAQEPRVLV
                     MAPPDHPAAKEAGVLFGAGMSLAPDAPASEADAAILIQLPEGNANIPKAGTLFLSLDA
                     YAALRGLEVRSHQVTKRPALRITAVAPWMPGLEVGHEISWFGKEGDRFTQRSLPAEGL
                     PDGARVLAVSTLDQGPILLEETLPDGRRLVALDLESPNGRAGYDPGSKLKWFFITRAL
                     DTKAFYGRHVPRRLTVDEYTQRLRELAARHTGRATLTEIGKGSAGDPILSLSLGKKDA
                     PRFVFTGVLHGGEVMNAYGLLRLAEVLLENPREDARISRLLGEYGVEIIPLLNAWGYA
                     HGKQTNSRDCDLNRNFDYLWEKFQGDSGWRAAIKPEVLRGPAPFSEGETQAVRDRLLA
                     GKVAGYIDFHQHGMEHGHMLMLPHRPVQRQPEVLRLLHEVLNARLAGRALYGSDQRLQ
                     LVLSGTGSPRPFAQNWAASRGITACTFELPGGVEESLILTDMVVQEALHFMWILAATS
                     PGS"
     gene            complement(5036..5212)
                     /locus_tag="PLY56_05630"
     CDS             complement(5036..5212)
                     /locus_tag="PLY56_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ20993.1"
                     /translation="MAHVINPDTCTSCGVCAEACPVEAISEGTDSYVIDADACTDCGT
                     CVEECPTESISEAA"
BASE COUNT          956 a         1820 c         1843 g          958 t
ORIGIN      
        1 atgaggtgtt cagcttccgc ctgcacggcc tgctatgagc gcgcagcagc gcgcagtctg
       61 gtgaacgcgg cacagaagga ggaaacgggt gtcatcccga aggaaggtac aagcacggtg
      121 gagggagcgc tgatagacgg gggcgcaagg tgagcgccag ttccgcatgc atcggcgctg
      181 gtcccccgcg ggacgacttg ccgctttcgc ccggcgcgaa cgcgagttct cctgctggcc
      241 tccggtcgcg tggatgagga gggatctcaa tggcaacacg gatgcggaag cgcggtttca
      301 cgcttatcga gctgctggtt gtgatcgcga tcatagccat cctggcggcg atcctcttcc
      361 cggtcttcgc ccgcgcgcgg gaaaacgcgc gcaagagcac gtgccagagc aacctgaagc
      421 agatcatgct gggcgttctg cagtacacgc aagactacga cgagtgcatg ccgatctatc
      481 gctggaatca cgccgcagtc cctagcgtgt gggtggatcg tgataacagc gcagcgaatg
      541 accgccactt ctggctggag gcgatccagc cctacctgaa gaacacccaa atgctctttt
      601 gccccagcca gagcttcgga ccaaccaccc tgagcaacgg gaagctgtat gtggcctacg
      661 cctacaacgg cgcgatgagt ggcaagagcc tggccctctt ccagagcacc gccgataaga
      721 tctgtattgg cgatatcggg gagaggcatg ccgaccgcac gcccactggc tacgtggaga
      781 actggttcat caactcggat ggcaccactt cctggaagca gtggtcctgg tggtgctcca
      841 tccacctcgg gggcggcaac tacggctatc tcgatggcca cgtgaaattc cacacggaga
      901 atgacaaggt tttgggccca gtcctcgcca ccggaagcag cctcccgggc gatcagaccg
      961 gttactggat gccctaagtc gaaggcgcgg ctgggatgga cgccgggcgc gcttgcgccg
     1021 cgaccctgcc catcctgtcg ctaccctcat ctttgatcta catgccctcg cttgcttgta
     1081 gcgagggcat gctttccctt gcgacttcgc ccagtcacac cttcccctgt accccgcatc
     1141 cctcgcttcc tacgtagccg cggaggatcc ctcctgctta ggcgaagcca ggcatcaccg
     1201 ctcccgatga aagcagaaac gggcacccgc gcggcgcttc ctatcgctcc tttcgcacac
     1261 aaggggatag gcggcacgcc gtggaatagc aatcgtgcgg agggcctgct ctggagtagt
     1321 gaagggacag tcatgactgc taaagaccat atgcgcggcc tggtcgagct ccccgagggc
     1381 gaccttcagg cagcggaacg catggtccgc ggcgtgaagc agtatgccgc gggcgcagag
     1441 acggcgccgt tggaggcgtt tgtgcggcag gcaacgcctg gggaggcgct ccccctgttc
     1501 gatgctcagg cgggattcgg tggggccagt aagggcgatc ccaccaccgt ctccgcggat
     1561 gattgcctgc gtgccatggc caaagtgagg gtgcaccgcg ccctcgttcg caccgaacca
     1621 gacacgctgg cagtggaccc gctcctcgcg aacgacctct tgctcgaggc gtgcgaggtc
     1681 tctgacagcc tggtgccctg ccccatcgtc ctccccgcga cgggcggcga cgtgccgccg
     1741 gaggccgacc aggtggacca cctgatccgc tgcggcgccg gcgcggcgat ggtccggccc
     1801 gcccaggatg actggcagtg gctccccttt gtttacacgc cgttgatgaa cgcgctctcg
     1861 gagaggcggc tgccgctctt ctgcatcgag cgccacgtct cgctagggca ggtggccgat
     1921 gtggcggcga gattcccgga tctgccgatc atcgtggcga acgtcggcta tcgcgcacag
     1981 cgcacgctgg tgcccctcct cgccacctat cggaacgtgc acctctctct cggaagcaac
     2041 tggaccgtcc accgcgggat cgagcagatg gtggagtggt tgggtccaga gcagcttctt
     2101 ttcggcaccg gcttcccggc ggcagagccg atgatggcga tcacacaact gatgtatgcc
     2161 gagatctccg aggaagcgcg ggcgctgatt ggctccggga acctggagcg cctgtggtcg
     2221 ggggtgaagc gatgaaaacc cttttggaac gtgcccgcag cggcgaatcg ctggcagatc
     2281 tcgagattgt ggatatgcac ggccacttcg ggcgttacct tcacgcgatc cccgacctca
     2341 ccgcagaggg cctggttcgc gtgatggatc gcctgggcgt ccggtcgatc ctggtgtcgc
     2401 acatgcagtg catgtcgcag cacacgcacc gtggcaacga ggaggtgctg acggcgatgc
     2461 gcgcctttcc cgggcggatc ctcggctatg ccgtggtctg gccggtggat ggcgacacgc
     2521 gcgcggagat ggagtggtgc gccgcacatg gctttacggg cctgaagctg cacagcagca
     2581 acggctttgc ctatacggat cctgtctatg ccggcgctct ctccgtggcc aacgaacggc
     2641 gcatgcccgt gctgctccac gcctggggcg ccgcctcgga tttcgcagcg atccgcgagc
     2701 tagcagcgaa atacccggaa gcgagctttc tcttcgcgca ctcgggcacc ggaggcgcgg
     2761 aagcggagtg catccgcttg gcgcgcgaag tgcccaacgc ctatctggac ctggcctaca
     2821 gccagggccc gcgtggcctc gtggagcggc ttgtcgccgg cgcgggcgcg gagaaggtcc
     2881 tcttcggttc cgattgctac ttcttcagca tgacccagca gatcggcaag gtcctcggcg
     2941 ccgatctctc tgatggcgag aagcgaaaga tcctctcgga gaacgcgctc cgcattctag
     3001 agcgcgtcca gcgctgaccg acggcgggat cacgtgcctt cactgccgtc aggtgtcgat
     3061 ggcgctcgca ggccgctgct tgccgacgga ggagcccaga gcggggagcc ggcaagaccg
     3121 attcgggggc gagtaagagc gaacatcatc tgcgcgaaag gagccggatc catgaccgtt
     3181 cgctattctt accgggcggg gctgatggcc gccatcctgc tgctcggcgg ggccggccag
     3241 acggcgcttc tcgcgggcca ggcggctggc gccgcgcgcg tctccggcga gcaagaaggc
     3301 gtgctgcagg taaaggacgg gaagctgcct gccgggccgg ccgagattcg ccagggaggc
     3361 gcggtggtcg gtttcgcgcg cgtggatccc gagggcgtcg cgcgtcgcgt gttgggccag
     3421 gcgccgcggg atgctgagat ccaccccatc gccgcccagg agccgcgcgt gctcgtcatg
     3481 gcgccgccgg atcaccccgc tgcgaaagag gcgggcgtct tgtttggagc tggaatgtcg
     3541 ctcgcgcccg acgcgcccgc gtccgaggcg gatgccgcca tcctgatcca gcttccggag
     3601 ggtaacgcca acatccccaa ggcaggcacg ctcttcctct cgctggatgc ctatgccgcg
     3661 ctccgcggcc tggaggtgcg ctctcaccag gtgacgaagc ggccggcgct gcgcatcacg
     3721 gcggtggcac cgtggatgcc ggggctggag gtcgggcacg agatctcctg gtttggcaag
     3781 gagggcgacc ggttcacgca gcgctcgctc ccggcggagg gcctgccgga tggcgcgcgt
     3841 gttctggcgg tgtctaccct cgatcagggc cccatccttc ttgaggagac gctgccggat
     3901 ggccggcggc tggtcgccct cgacctggag tcgccaaacg gccgggcggg ctacgacccc
     3961 ggaagcaagc tcaagtggtt cttcatcacg cgcgcgctcg atacgaaggc tttctatggg
     4021 cgccacgtcc cgcgccggct caccgtggat gagtatacac agcggttgcg cgagctcgcg
     4081 gctcgccaca ccgggcgcgc gacgctcacg gagataggga aaggctccgc cggcgatccg
     4141 atcctctcgc tctcgcttgg caagaaggac gcgccccgct tcgtcttcac cggcgtcctc
     4201 cacggcggcg aggtgatgaa tgcctatggc ctcctacgcc tggccgaggt gctcctggag
     4261 aacccgaggg aggatgcgcg gatctcgcgc ctgttggggg aatatggcgt tgagatcatc
     4321 ccgctgctca acgcctgggg ctatgcccac gggaagcaga cgaacagccg cgactgcgac
     4381 ctgaaccgca atttcgacta cctgtgggag aagttccaag gcgatagcgg gtggcgggcg
     4441 gcgatcaagc ccgaggtgct gcgcgggccg gcaccctttt ccgaaggcga gacgcaggcc
     4501 gtgcgcgacc gtctgctggc gggaaaagtg gccggctaca tcgactttca ccagcacggc
     4561 atggagcacg ggcacatgct gatgctcccg caccggccgg tgcagcgcca gccggaggta
     4621 ctccgtttgc tgcatgaagt cctgaacgcc cgcctggccg gccgcgccct ctacggaagc
     4681 gatcagaggt tgcagctcgt tctcagcggc acgggctcac cgcggccctt cgcgcaaaac
     4741 tgggcggcct cgcgcgggat cacggcctgc accttcgagc tgcccggcgg cgtcgaggag
     4801 agccttatcc tgaccgatat ggtggtccag gaagcgctcc atttcatgtg gattctggcc
     4861 gccacatcgc cgggttcgtg accccgcccg tggatccacg ggcgggagag cgtgcctctg
     4921 cgttccgaga tctccgagca ggccattgcg tacacgagac ggaccggccg cgcaatcgct
     4981 gcggccggcc cttattcgtg ggcctcatcc ggcaaggccg gacgaggggg ctgcattagg
     5041 cagcctcgct aatcgactcg gtcgggcact cctccacaca ggtgccgcag tccgtgcaag
     5101 catccgcgtc gatgacgtag gaatcggtgc cttcagagat cgcctcgacg gggcaggcct
     5161 cggcgcaaac cccgcacgac gtgcacgtat ccggattgat cacgtgagcc atagtttaac
     5221 gagagtcctt tctacaaaat cgcaaactcg agaccgggcc acgcccagcc ggcagcgtgt
     5281 cgcatcgccg accccattat agcacattcc tggcaagagt gctccctatt tccagaaaat
     5341 agcgcggatt ccgagaaaac cgcgaacccc gcacgatagc cccctgtcgg ttgcgcggct
     5401 ctgagtgccc gcgccccacg gcgccaactg tttcgcaacc gaagctggaa ttccggcata
     5461 aggagagggc cgcccacacg cgcggagctc gaagctccgc gctgctttcc ggcaaagccc
     5521 cctcggggct tctcctccca gccaccgccg ttttgagaca tctgggagta tccccaa
//
LOCUS       DAOSCV010000171         5539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9250_length_5539_cov_3.587710, whole genome shotgun sequence.
ACCESSION   DAOSCV010000171 DAOSCV010000000
VERSION     DAOSCV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9250_length_5539_cov_3.587710"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLY56_05635"
     CDS             <1..642
                     /locus_tag="PLY56_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20994.1"
                     /translation="RGRTLFYLYVPDPAVPIKARIRHQAGKQRNEPIAYAIIDSRGVP
                     LTDDLIQPGETATVTRKAPEPGVYALLVEASGGGGPWYDVSIPEHPYVVGGKKEVYFF
                     RGVSRQYFLVPEGTASFRIHFATGDQNQQARLQLFNSRDERVLDETLISDQGAKKRFE
                     VKVAPEEQGKVWSLLIGRPEKMPSPYYSENYYLRLEGIPSYFSDRPDGLLMPR"
     gene            939..2168
                     /locus_tag="PLY56_05640"
     CDS             939..2168
                     /locus_tag="PLY56_05640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ20995.1"
                     /translation="MLARDVTEAVHLNATAAHVARMALEGISEAASVAALRRRYQGVG
                     DPREEVACIYALVDAFRAPEPRCPRCAFPSLERIPAFSAPVSAPYKVDLALTYRCNNA
                     CAHCYNEPGRKAMPSLTTAQWREGLDRLARLGVLHVIFTGGEPTLFPDLIELIRHADH
                     LGPIVGMNTNGRRLAGPGYARALARAGLSHVQVTLASCRADEHNAITGAKSFDETVAG
                     IRSALESGLHTITNTTLTQRNAGHAVEIVDFLHALGIHTFAMNGMIYSGRGAADPNAL
                     PASEMAPLLARVRDRAEELGMRFLWYTPTEYCQLSPLELRLGMKRCNAGEYSCCIEPN
                     GDVLPCQSYYQPAGNLLRDPWERIWESPLFRAFRNRGSEPEAAGLPEKCRHCPDFPVC
                     GGGCPLEREARARRGEC"
     gene            2171..3472
                     /locus_tag="PLY56_05645"
     CDS             2171..3472
                     /locus_tag="PLY56_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ20996.1"
                     /translation="MPSRNWLQQAFRLQRPEPPPPIPPGLYPFMQETDGAYTRFHLRV
                     EPDGTGTLVANASAAVRLTPSGVVMVKALLEGRQTEEILHDVLALFRGVTPERAREDL
                     GRVQTLLGRLLSPKDRYPILNLQDVALSLHQARLMAPFSADLPLADPVRILPLLARLW
                     DAGIFHAVFLAPDHPNPDHLVRAVERAEDLGMIAGVRARASDLAADRLLQELAQAGVD
                     HLTIPVASLGREIHDGFYGEGDHAAFERAIAVARANEVCPMVEVPLVESTVDALDPLL
                     EWLEGLAIAEVSFFAIAAPDEMPAAQRSDALPASMLPQVAALVEGAARDTRVRFFWQT
                     PMRRDPLQPLADQVREGPRCSGDVAIRVEIDGSVIPPRGPYQPAGNLLRDPWPQIWES
                     QAFRLFRERVEAPSHCPDCPGLAICAAACPRDPADWAEPGR"
     gene            3499..5475
                     /locus_tag="PLY56_05650"
     CDS             3499..5475
                     /locus_tag="PLY56_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20997.1"
                     /translation="MHCRSPWLFLRCSFLLLLALLCVAPAGAQEYSFSVPQLRMQVQV
                     QEDASVRITYDITFANNPGARAIDVVDIGVPHAGYDLANMSASLEGVPLTDIRPSEYV
                     TPGVEVHLGNQAIPPGGERRFHFEFTMPEMVYQDTTREDYASLQITPTWFGEEFVTGT
                     TYLQIAVVTLPGIRPDEVLYQETPFDNKVEYAGGTAVLWRWTDAKATGPRLVGLSFPK
                     RGLSRVVQISRFELLVRRFAESPGARFWAGVASLFLLALLYFRFTGGTGCVLFVPLFI
                     AFFILISASPAAHLLSFAPLIVLVLWNEWYLARRRRRYLPPVAQVEGGGVLRGLSPAE
                     AAVLLEMPLKKVLLLVLFGLLRKGVLREVRSSPWELEVVEAFRTYGAGLPDRKAERLR
                     AARDTGGVIHPYEHAFLDVLEKNRGKPIQAIDFGGAMKKLIESTVGKMEGFDLSDTQD
                     YYRARVAHALAEAKAAVQPEERQSAIDRNLEWILLDRNPGPIFTGGGYGYRPVWTRRA
                     GGEARAPQAPPEEAASTPFSPSQQGRAGEGAASTSFGVSRPQAPGEEAASTSFAGWRE
                     GKGAGAASTSFADWADQTAEELSGAIPSGSVRIEGARGGIIDLSAADRATADLFEALS
                     ESSGGGGGGGGGGCVCACACAGCACACACAGGGR"
BASE COUNT          866 a         1904 c         1833 g          936 t
ORIGIN      
        1 cgcgggcgca cgctgttcta cctctatgtg cccgaccccg ccgtcccgat caaggcgcgc
       61 atccgacatc aggcgggcaa gcagcgcaac gagccgatcg cctacgcgat catcgactcg
      121 cgcggcgtgc ccctgaccga tgacctgatc cagccgggcg aaacagcgac ggtgacacga
      181 aaagcgcccg aacccggcgt gtatgccctc ctggtagagg cgagcggagg cggagggcca
      241 tggtacgacg tctccattcc agagcacccc tacgtggtgg ggggcaagaa ggaggtctac
      301 ttcttccggg gggtgagccg gcaatacttc ctcgtgcccg agggaaccgc gtcgttccgg
      361 atccattttg ccaccggaga ccagaatcag caggcgcgcc tccagctttt caacagccgc
      421 gacgagcgcg tgctggacga gacgctcatc tccgaccagg gcgcgaagaa gcggttcgag
      481 gtgaaagtcg ccccggaaga gcaggggaag gtctggtcgc tcctgatcgg ccggcccgag
      541 aagatgccct cgccctacta ttcggagaac tactacctcc gcctggaggg catccccagc
      601 tacttcagcg accggcccga tggcctgctc atgcctcgct aaagggaggg cggtcgagac
      661 cagcacgcgc cggctcgcgt caggacaggg cacctggggc tctctggcga atggggtggg
      721 ggggattcaa gcgatgggct ctcgtgtgcc gataggagtc gcgtgatgac ggattgtgcg
      781 tccctgatcc gccgcgcggc cggcaacctg cgcaaagagg tgcaggaact gcgtgctgcc
      841 tccagccgcc accggcgccg gggctctaca cctatcggct cgaacaggag agccaccacg
      901 ggcgggtcca cctgcgtatt catccagacg ggagcgggtt gctcgcccgc gacgtgaccg
      961 aggcggtgca tctgaacgcc accgcggccc atgtggcccg gatggcgctc gaaggcatat
     1021 cggaagccgc gagcgtcgcc gcgctgcgcc ggcgctacca aggcgtgggt gacccgcgcg
     1081 aggaagtggc atgcatctac gcgctcgtgg atgcattccg cgctcctgaa ccccgatgtc
     1141 ccaggtgcgc cttcccctcc ctggagcgaa ttcccgcatt cagcgcgccc gtgagcgcgc
     1201 cctacaaggt cgatctcgcg ctcacctacc ggtgcaacaa cgcctgtgcc cactgctaca
     1261 acgagcccgg gcgcaaggcg atgccctcgc tcaccaccgc ccagtggcgc gaagggctcg
     1321 accgcctggc gcgcctgggt gttctccacg tcatctttac cggcggcgag ccaacgctct
     1381 tcccggacct gatcgagctg atccggcacg ccgatcacct gggtcccatc gtgggcatga
     1441 acaccaacgg ccgccggctg gccgggccgg gctacgcgcg cgcgttggcc agggccggcc
     1501 tcagccacgt tcaggtgacg ctcgcctcat gccgggccga tgagcacaat gcgatcacgg
     1561 gcgcaaagtc gttcgacgag acggtggccg gcatccggag cgccctggag agcggtctcc
     1621 acacgatcac caacaccacc ctcacccagc gcaacgcggg gcatgccgtg gagatcgtcg
     1681 acttcctgca tgcgctcgga atccacactt tcgccatgaa cgggatgatc tactcgggac
     1741 gcggtgccgc ggatccaaac gccctcccgg caagcgagat ggcgccgctg ctggcacgcg
     1801 tgcgcgaccg ggccgaggag ctggggatgc gcttcctctg gtacacgccg acggagtatt
     1861 gccagctttc gccgctggag ctgcgcctgg ggatgaagcg gtgcaacgcc ggcgagtatt
     1921 cgtgctgcat cgagccgaac ggcgacgtgc ttccctgcca gtcgtactac cagccggcgg
     1981 gcaatctgtt gcgcgatccg tgggagcgca tctgggagag cccccttttc cgcgctttcc
     2041 gaaaccgcgg ctccgagccg gaagcagccg gtcttccgga aaagtgccgc cactgtcccg
     2101 acttcccggt gtgcgggggt ggctgccccc tggaacgcga ggcgcgcgcc cggagaggag
     2161 agtgttgacc atgccttcgc gcaactggct ccagcaggcg ttccggcttc aacgcccaga
     2221 accgccgccg ccgatccccc cgggcctcta tcccttcatg caagagacgg atggagcgta
     2281 cacgcgcttt cacttgcgcg tcgagccgga tggcaccggc accctcgtcg ccaacgcctc
     2341 ggccgccgta cgcctgaccc cttctggcgt ggtcatggtg aaggcgctgc tggagggccg
     2401 ccagacagaa gagatcctcc atgacgtgct ggcgctcttc cgcggagtca cgcctgagag
     2461 agctcgcgag gacctcggcc gggtacagac gctgttggga cggctcctct cgccaaagga
     2521 ccgctacccc atcctgaatt tgcaggacgt cgccctctcg ctgcatcagg cgcgcctcat
     2581 ggctcccttc agcgccgatc tgcctctggc ggatcccgtg cgcatcttgc ccctcctcgc
     2641 gcggctctgg gacgccggca tcttccatgc cgtctttctc gctcccgacc accccaaccc
     2701 ggatcacctc gtgcgcgccg ttgagcgagc cgaggacctg gggatgatcg ccggcgtgcg
     2761 cgcgcgcgcc tccgacctgg cggccgatcg gctcctccag gagcttgccc aggccggcgt
     2821 cgatcacctg acgatcccgg ttgcgtctct gggcagggag atccatgacg gtttctacgg
     2881 ggagggcgat catgccgcct tcgagcgggc catcgcggtc gcgcgcgcca atgaggtgtg
     2941 cccgatggtc gaggtgcccc tggtcgaatc gaccgtggat gccctcgatc cgttgctgga
     3001 gtggctggag ggactggcga ttgccgaggt ctccttcttc gccatcgctg ccccggacga
     3061 gatgccggcg gcgcaacgca gtgacgccct gccggccagc atgcttcccc aggtagccgc
     3121 cctggtcgag ggcgccgcgc gcgacacgcg ggtgcgtttc ttctggcaga cgccgatgcg
     3181 ccgagatccg cttcaacccc tcgctgacca ggtgcgcgaa ggcccacgct gctcgggaga
     3241 tgtggcgatt cgcgtcgaga tcgacggctc cgtgattccg ccgcgcgggc cctaccagcc
     3301 ggcaggcaac ctgctgcgcg atccctggcc gcagatctgg gagagccagg cttttcgcct
     3361 cttccgcgag cgcgtagagg cgccatcgca ctgccccgat tgtcccgggc tggcgatctg
     3421 cgcggcggcc tgcccgcgcg atccggccga ctgggcagag ccggggagat agccatgtcc
     3481 ctcggatgga gtgaacggat gcactgtaga tcgccgtggc ttttcctgcg ttgtagcttc
     3541 ctgctcctcc tcgcgcttct gtgcgtggcc cccgcgggcg cgcaggagta ctctttcagt
     3601 gtgcctcagc tcaggatgca ggtgcaagtg caggaagacg cttccgtgcg gatcacctac
     3661 gatatcacct ttgccaacaa cccgggagcc cgcgccatcg acgtggtcga tatcggagtg
     3721 ccgcacgccg gctatgatct cgccaacatg agcgcctccc tggagggcgt ccccctcacc
     3781 gatatccggc catcggaata cgtcaccccc ggcgtagagg tgcatctggg caaccaggcg
     3841 atcccgcccg gcggcgagcg ccgcttccac ttcgagttca ccatgccgga aatggtatat
     3901 caggacacca cacgagagga ctacgcctcg ctccagatca cgccgacgtg gtttggcgag
     3961 gagttcgtca ccgggaccac gtatcttcaa attgccgtag tcaccctccc cggaattcgg
     4021 ccggacgagg tcctctacca ggagacgccc ttcgataaca aggtggagta tgccggtggc
     4081 accgccgtgc tgtggcgctg gacagacgcg aaagcgaccg gcccgcgctt ggtgggtctc
     4141 tccttcccaa agcgcgggct atcacgagtg gtccagatct cgcgtttcga gcttctggtg
     4201 cgaagattcg ccgagagccc gggcgcgcgc ttctgggccg gcgtggcctc gctctttctt
     4261 ctcgcgctcc tctacttccg tttcaccggg ggaacgggct gcgtcctctt cgtgcctctc
     4321 ttcatcgcgt ttttcatcct gatcagcgcg agccccgcgg cgcaccttct ctcgttcgcg
     4381 ccgctcatcg tgctcgtcct gtggaacgag tggtacctgg cgcgccggcg ccggcgctat
     4441 ctgcccccgg tggcccaggt ggagggcggg ggcgtgctcc gcgggctgag ccccgcagag
     4501 gcggccgtgc tcctagagat gccgctgaag aaggtccttc tcctcgtcct cttcggcctg
     4561 ctgcgcaagg gcgttctgcg cgaggtgcgc tcttcgccgt gggagctgga ggtggtggag
     4621 gcgttccgga cgtatggcgc cgggctgccc gaccgcaagg cagagcgcct ccgtgccgcc
     4681 cgagataccg ggggtgtgat ccacccctac gagcacgcct tcctggatgt gctggagaaa
     4741 aaccgcggga agccaatcca ggcgatcgac tttggcggcg ccatgaagaa gctgatcgag
     4801 agcaccgtgg gcaagatgga gggcttcgac ctttcggata cccaggatta ctatcgcgct
     4861 cgcgtggcac atgcgctcgc cgaggcgaaa gctgccgtcc agccggaaga gcgccaatcg
     4921 gccatcgacc gaaacctgga gtggatcctt ctcgatcgca acccgggccc gatcttcacc
     4981 gggggtgggt acggataccg cccggtctgg acccggcgcg cggggggcga ggctcgggca
     5041 ccgcaggcac ctcccgagga ggcagcctcc actcccttct ccccctcgca gcaggggcgc
     5101 gccggcgaag gagccgcctc tacctcgttc ggagtctctc ggccgcaggc ccccggcgag
     5161 gaagcggctt ccacctcgtt cgccggatgg cgcgagggaa aaggcgcggg agctgcttct
     5221 acctccttcg cggactgggc tgaccagacg gcggaagagc tttctggcgc cattcccagc
     5281 ggttctgtgc ggattgaggg cgcccgaggt gggatcatcg acctcagcgc cgcggaccga
     5341 gccaccgccg atctcttcga ggcgctcagc gaatcctctg gcggaggagg aggcggtggt
     5401 ggcggcgggt gcgtctgcgc gtgtgcctgt gccggctgcg cgtgtgcctg cgcctgtgcc
     5461 ggcggcgggc ggtgaggcgg tgttccaccc aggagaagcg agcgagcgca acctcctccg
     5521 catgcaggca gcctggctg
//
LOCUS       DAOSCV010000172         5535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9258_length_5535_cov_4.036861, whole genome shotgun sequence.
ACCESSION   DAOSCV010000172 DAOSCV010000000
VERSION     DAOSCV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9258_length_5535_cov_4.036861"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLY56_05655"
     CDS             <1..605
                     /locus_tag="PLY56_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ20998.1"
                     /translation="AILFPVFARARENARKSSCQSNLKQIGLAVQQYAQDYDETYPGL
                     YISSPSAVSYVLPNGTNYTSQYVLWPTLIYPYVKNIQVYNCPSCNHRWTGTYSGTMCY
                     GINEQSFGSAGRAMAAINYPSELMYFADSNTAASNSYRFDITTGNPDTNGDGINDYKI
                     DMRHSDGANVCYTDGHVKWIKINAVPAYSTSSRFWRYDAP"
     gene            complement(731..2929)
                     /locus_tag="PLY56_05660"
     CDS             complement(731..2929)
                     /locus_tag="PLY56_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ20999.1"
                     /translation="MSRTHTWEFPLPRTHTGVLLGNGTLGAIVWGAGPELRITLGRAD
                     HWDHRGGMPWTEKHSYRAIRECLEQGDEQRLRALFEEKVREGEPRRPTVLPVGRVDLH
                     LGEGATLQSATLEMERGELVVTLEKAGETCTLRVVMAMDRPVLLLLLPDALKDARARS
                     VPAWEYVGEELKKVSYEPPVVLEGGEIAGWVQACPADPALCAGYRQEGSILSIAVERG
                     ESVAAARDRAARRVADALARGYREEAEASRLWWQAYWDSVPRVEIPNETLSFLYDYGM
                     YKFAAFTNPDGVPATLQGAWVEEYRLPPWSNDYHFNINVQLCYQPAYHGNRLENLRPL
                     FQMIDSWREVLRHNARVFVGIDDGVMLPHAVDDRCTCMGGFWTGSIDHGCTAWVALMM
                     YRYYRYTLDRDFLRETAYPFMHGAMRVYEEMLEREGERLLLPVSVSPEYRGAQMNAWG
                     RNASFQLAAIHALCEALEQAAADLGEEPRPIWREIRERLPRACLMDGQIILWEGTPLE
                     ESHRHHSHLAGITPFDILDLEDPVWRGHIERSLRHWILMGPGLWSGWCVPWASMIHSR
                     VGNAEAAELLLEVFDRFYTNEGHGTLHDAAAAGFSLMGMPAIQGPPSRPDLMQMDGGM
                     GAVAAVQEMLLHTRRGVNYLFAGAPARWKRVSFERMRTDGAFLVSAAREKGVVEYVTV
                     ESEAGGVFRARNPWPGAARVHRASGEERVEGTFLEIAMHPGERVVLRPAG"
     gene            complement(2932..4104)
                     /locus_tag="PLY56_05665"
     CDS             complement(2932..4104)
                     /locus_tag="PLY56_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21000.1"
                     /translation="MSVQPQIASTEHFWYRLQPEGPYIDTQRDNKAFGFKDGRIYLSE
                     DCGHTWPYSSEFPEAEKITFSVILKNGNILFATGAQLYLSTDNLKSYHPVTVKNPDGS
                     DYLPHTPQNPENPGWYFHPLNGVKSWDVNGIEMLVWGNYCNVLGGASPANIYYSVDGG
                     ETVKIAYAFGQNPYFRDNGSPGGGREGTLLGNPDNPVICRHVHTVAYNPDENAFYACT
                     GDGDRPEGFECHWLRGTYDAAADRWDWKVVHSDHLNSRYKGGGITFVDGMLYWISDSN
                     GPEPYDRGIFRCAPEDLTHPERHERLFNPEVESANMVIQDNVILASHCAPASPMATGI
                     IVSLDGGKTWAQHDLAELGQRSPCRFHEKNTEGWFRVDLRAGWIERAEVLFIKPKE"
     gene            4285..4809
                     /locus_tag="PLY56_05670"
     CDS             4285..4809
                     /locus_tag="PLY56_05670"
                     /inference="COORDINATES: protein motif:HMM:NF015142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HOJ21001.1"
                     /translation="MRYCIRISLLLLLFSFSAGGLAAPATKALAGIPNFHTVAPGIYR
                     GGAPTREGLKALSKMGVRTIIDLRIEKQAKRVEKPLAEKMGFTWIHIPMGREAPTEKQ
                     VATFLATLAKAPERPVFVHCQHGADRTGCMIGIYRVQVQKWPFERAWAEMLRYGFKPW
                     LHELKAAVRSRARS"
     gene            5046..>5535
                     /locus_tag="PLY56_05675"
     CDS             5046..>5535
                     /locus_tag="PLY56_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ21002.1"
                     /translation="MGSPHTNGSTAKLLEALLASAREAGAQTERVDLAGLKMEFCRGC
                     VQCYRTGRCVRKDDVEQIKEQMLAADGIVLGSPVYIRSVSAQLKVLMDRCAYFVHCFL
                     LEGKYGAAVATAGGADQEETAEFANGFLRMCGAYTVGTASALSDGANSVREPETALAQ
                     AAA"
BASE COUNT          951 a         1883 c         1727 g          974 t
ORIGIN      
        1 ccgcgatcct ctttccggtg ttcgcgcggg cgcgggagaa cgcgcgcaag agctcgtgcc
       61 agtccaacct gaagcagatc ggtctcgccg tgcagcagta cgcccaggac tacgatgaga
      121 cctatccggg actctacatc tcatcccctt ccgcggtcag ctatgtgctg ccgaatggca
      181 ccaactacac cagtcagtat gtcctctggc ccacgctcat ctatccttac gtgaagaaca
      241 tccaggtgta caactgcccc tcgtgcaatc acaggtggac aggcacctac tcggggacga
      301 tgtgctacgg catcaacgag caatccttcg gttcggcggg ccgggccatg gccgcgatca
      361 actatccctc ggagctgatg tatttcgccg attcgaacac ggcggcatca aactcgtatc
      421 gcttcgatat caccacgggc aatcccgata ccaacggcga cgggatcaac gactacaaga
      481 tcgatatgcg ccattccgac ggcgccaacg tctgctacac agacggccac gtgaagtgga
      541 tcaagatcaa cgcggtgccc gcgtactcga catccagccg cttctggcgc tacgacgcgc
      601 cgtagacggt tctgcgcgct ggtgggaccg gctggctcca gtttcccagc gcgcaataaa
      661 caagtagcaa cagcagccgc cttgcgcgag gcggctgcct tagtatccca gagccgctgg
      721 agttcctcac ctatccggcg gggcgcagca cgacgcgctc gcccgggtgc atcgcgatct
      781 ccaggaacgt gccttcgacc cgctcctcgc ccgatgcccg atggactcgc gccgcccccg
      841 gccacgggtt gcgcgcgcgg aagacacccc ccgcttcgct ctccaccgtg acgtactcca
      901 cgacgccttt ctctcgtgcc gcgctcacca ggaaagcccc gtcggtgcgc atcctctcga
      961 acgagacgcg cttccaccgg gcaggcgcac ccgcaaagag gtagttcacc ccccggcgcg
     1021 tatgcaacag catctcctgg accgccgcca ccgctcccat ccccccatcc atctgcatca
     1081 ggtccgggcg gctagggggc ccctggatcg ccggcatccc catcagcgag aatccggctg
     1141 cggcggcatc gtgcagcgtg ccgtgcccct cgttcgtgta gaaccggtcg aacacctcca
     1201 ggaggagctc cgctgcctcg gcgttcccca cgcgactgtg gatcatcgaa gcccatggca
     1261 cgcaccaccc cgaccagagc cccggtccca tcaagatcca gtggcgcaga gagcgctcga
     1321 tgtggccccg ccacaccggg tcttccaggt cgaggatatc gaagggagta atcccggcga
     1381 ggtgcgagtg atgccggtgg ctctcctcca gaggagtgcc ctcccagagg atgatctggc
     1441 catccatcag gcacgcccgc ggcaggcgtt ctcggatctc ccgccagatc ggccgtggct
     1501 cctcgcccag atcggcggcg gcctgctcca gcgcctcgca aagcgcatgg atcgcggcga
     1561 gctggaagct ggcattgcgc ccccatgcgt tcatctgcgc gcctcgatac tccggcgaca
     1621 cgctcaccgg gaggaggagc ctctctccct cgcgctccag catctcctca taaacgcgca
     1681 tcgccccgtg catgaagggg tatgcggtct cgcgcaagaa gtcgcgatcc agggtgtagc
     1741 ggtagtagcg gtacatcatc agcgccaccc acgccgtaca cccgtggtcg atgctcccgg
     1801 tccagaaccc gcccatgcag gtacaccggt catccaccgc gtgcgggagc atcacgccgt
     1861 cgtcgatgcc cacgaaaacc ctcgcattgt gccgcaacac ctcgcgccac gagtcgatca
     1921 tctggaagag agggcggagg ttctccagac ggttcccgtg gtaggcaggc tggtagcaga
     1981 gctgcacgtt gatgttgaag tgatagtcgt tcgaccaggg gggcagccgg tactcctcga
     2041 cccacgcgcc ctgcaaggtg gccggcacgc cgtccggatt ggtgaaggcc gcgaacttgt
     2101 acatcccgta atcgtacagg aaggacaagg tctcgttggg gatctccacg cgcggcaccg
     2161 aatcccagta ggcctgccac caaagccggc tcgcttccgc ctcctcgcgg tagcctcggg
     2221 cgagggcatc ggccacccga cgggcggcgc ggtcgcgggc agctgccacg ctctctccgc
     2281 gctcgacggc gatagagagg atgctccctt cctggcggta gccggcgcag agcgcgggat
     2341 ccgccgggca ggcctgcacc cacccggcga tctcccctcc ctcgagcacc accggcggct
     2401 cgtaagagac ttttttcagc tcctcgccca cgtactccca cgcgggcacg ctccgcgccc
     2461 gcgcgtcttt cagcgcgtcc ggcagcaaga ggagcagcac cggccggtcc atcgccatca
     2521 ccacgcgcag cgtgcacgtt tcgcccgctt tttcgagcgt caccaccagc tcgccgcgct
     2581 ccatctcgag cgtggcggat tgtagggtgg ccccctcacc caggtgaagg tccacccgac
     2641 ccacggggag gacagtgggc cggcgcggct cgccctcgcg gactttctcc tcgaacagcg
     2701 cgcgcaggcg ctgctcgtcg ccctgttcca ggcactcgcg gatggcccgg taggagtgct
     2761 tctcggtcca cggcatcccg ccgcggtgat cccagtggtc ggcgcggcca agcgtgatcc
     2821 gcagttccgg gccagcgccc cacacgatcg cgccaagcgt gccgttgccc aggagcacgc
     2881 ccgtatgcgt ccgcggcagc gggaactccc acgtgtgggt cctgctcatg atcactcctt
     2941 cggcttgatg aagagcacct cagcgcgctc gatccagccg gcgcgcaggt ccacccggaa
     3001 ccaaccctcg gtgttcttct catggaaccg gcagggcgag cgctggccaa gctccgccag
     3061 gtcgtgctgc gcccacgtct tgcctccgtc cagcgagacg atgatcccgg tagccatcgg
     3121 cgaggccggc gcgcagtggg aggccaggat gacgttgtcc tggatgacca tgttggcaga
     3181 ctccacctcg gggttgaaga gacgctcgtg cctctcggga tgcgtcaggt cctcgggcgc
     3241 gcaccggaag atcccccggt cgtacggctc gggcccgttg gagtcgctga tccagtagag
     3301 catcccgtcg acgaaggtga tcccgcctcc cttataccgc gagttgaggt ggtccgagtg
     3361 caccaccttc caatcccagc gatccgccgc ggcgtcgtag gtgccccgca gccagtggca
     3421 ctcgaagccc tcgggccggt cgccatcgcc ggtgcaggcg tagaacgcgt tctcgtcggg
     3481 gttgtaggcg acggtgtgga cgtgccggca gatcaccggg ttgtccggat tgccgaggag
     3541 cgtgccctct cggcccccgc caggcgagcc gttatcgcgg aaataggggt tttggccgaa
     3601 ggcataggcg atcttcaccg tctccccgcc atcgaccgag tagtagatgt tcgccggcga
     3661 agctccgccc aggacgttgc agtagtttcc ccacaccagc atctcgatgc cgttcacgtc
     3721 ccacgacttc acgccgttca gcgggtggaa gtaccagccc gggttctccg gattctgcgg
     3781 ggtgtgcggg aggtaatcgg aaccatccgg gttcttcacg gtcaccggat ggtagctctt
     3841 gaggttatca gtgctgaggt agagctgcgc gccggtggcg aagaggatat tgccattctt
     3901 caggatgacg ctgaaggtga tcttctccgc ctcgggaaac tcggagctgt agggccaggt
     3961 gtggccgcaa tcctcggaaa ggtagatccg cccatccttg aagccaaacg ccttgttatc
     4021 acgctgcgtg tcgatgtagg ggccttctgg ctgaagccgg taccagaagt gctcggtcga
     4081 agcgatctgc ggttgtacgc tcatcgatgt gtacctttca ggccctgctg ccgggagcga
     4141 gcccggccag cgcgggcact ccccatctgc tcctcttgct ccttcagacc ggtttggagc
     4201 gccttgacgc gggctcgggg cgttgggtag aatgaagccg gcgctcgcgc cccaccccgc
     4261 tttcgacgaa ttggaggagc gtcaatgagg tattgcatac ggatctcgct tctcctgctg
     4321 ctcttctcct tctcggcggg cgggcttgcc gcgcccgcga ccaaggcact cgccggcatc
     4381 ccaaacttcc acacggtcgc ccccggcatc taccgcgggg gtgccccgac acgcgaaggc
     4441 ttgaaagcgc tctcgaagat gggcgtgcgc acgatcatcg acctgcggat cgagaagcag
     4501 gcgaagaggg ttgaaaagcc gctcgccgag aagatgggct tcacctggat tcatattccg
     4561 atgggccgcg aggcaccgac ggaaaagcag gtcgccacct ttctcgccac gctcgcgaag
     4621 gcgcccgaga ggccggtctt cgtccactgc cagcatggcg cggatcgcac cgggtgcatg
     4681 atcgggatct accgggtgca ggtgcagaaa tggccgttcg agcgtgcgtg ggccgagatg
     4741 ctccggtacg ggttcaagcc ctggcttcac gagctgaaag ccgccgtgcg ctcccgcgcc
     4801 cgctcctagt ccgcattatt catcagaata cttcgggcta gagggcatcg ccggcccctc
     4861 accttgggag gagccggctt ttctggcgcc gaatgggtgt ggtggatccg gctcggaccg
     4921 gctcatgccc cggcatcacc cggatccgtt cacttcatag aggggtggcc ggcacctcag
     4981 acgtatcgtg ggttccgtgc cccctcggcc tattgaaagt cacgcttatg agaatccttg
     5041 gtatattggg tagcccacac acgaacggta gcacggcaaa gctgcttgag gcgctccttg
     5101 cgagcgcgcg ggaggcaggc gcgcagacag agcgcgtgga cctcgccggc ctgaagatgg
     5161 agttctgccg tggctgcgtg cagtgctatc gaaccggccg atgcgtccgc aaggatgacg
     5221 tggagcagat caaggagcag atgctcgcgg ccgatggaat cgttctgggc agccccgtct
     5281 acatccgaag tgtgtcggcg cagctcaagg tgctcatgga tcgatgcgcc tacttcgtcc
     5341 actgcttctt gctggagggc aagtatggcg ccgcagtagc cacggcgggc ggcgccgacc
     5401 aggaggagac ggcggagttt gccaacggct tcctgcgcat gtgcggtgcc tacacggtag
     5461 gtaccgccag cgccttgagc gatggcgcca actccgtgcg cgagccggaa accgccctcg
     5521 cgcaagcggc ggctt
//
LOCUS       DAOSCV010000173         5533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9266_length_5533_cov_3.621577, whole genome shotgun sequence.
ACCESSION   DAOSCV010000173 DAOSCV010000000
VERSION     DAOSCV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_5533_cov_3.621577"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="PLY56_05680"
     CDS             complement(<1..864)
                     /locus_tag="PLY56_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21003.1"
                     /translation="MNNPYQSLDWQHVIRVASTPHMHLAAPWENPPLSPRRRLENARR
                     HGLRHFPISNYYPSAPCTPSTRLSDFRLRQAWSTTRNGVPVPAPINWNELITWQDELE
                     EEYRQQLPFQETDVLFPDLPADILLSKNAEHHSFTNSPCHITCPGSAFCSGNFDARNR
                     FRLRDHGYPTGYAGPWQEGFREMIEHLEYPDGGGIVIAHPTWSHLTDAAVLEMLDFSP
                     CVLGIEIYNDGVARGIREGRYPLEEGEPVPGYSTHMWDRILSTGRQCLGFAVPDHNIG
                     EQGDWLGRVVLL"
     gene            1331..1591
                     /gene="hfq"
                     /locus_tag="PLY56_05685"
     CDS             1331..1591
                     /gene="hfq"
                     /locus_tag="PLY56_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOJ21004.1"
                     /translation="MTKPQVNLQDAFLNQVRKENTGVVIYLINGAQIKGMVRGFDQFT
                     VVLESQGRLQLVYKHAIASVVPTKPIPAMNSESASRERAHIQ"
     gene            1588..2430
                     /gene="dapF"
                     /locus_tag="PLY56_05690"
     CDS             1588..2430
                     /gene="dapF"
                     /locus_tag="PLY56_05690"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ21005.1"
                     /translation="MNFLKYHGIGNDFILVDGFKEELPEELLSPQAQRLCDRHFGVGG
                     DGLILVLPSRVADFRMRMFNPDGSESEMCGNGIRCFAKYLYDYGLTRETQVTVETLAG
                     IIAPKMIVTDGKVQMVCVDMGEPRLARQEIPMEGGDAGSRVIAEKIDVDGERFEVTAV
                     SMGNPHCVTFVEDVEHFPVAKIGPLIERHPLFPRRTNVEFIQVLSNEELQMRVWERGA
                     GETLACGTGACASAVAAALNHRTGRKVTIHLPGGDLQIEWAGDNHVYMTGPAEEVFSG
                     TIAL"
     gene            complement(2525..3592)
                     /locus_tag="PLY56_05695"
     CDS             complement(2525..3592)
                     /locus_tag="PLY56_05695"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOJ21006.1"
                     /translation="MAKTYNIAVIPGDGTGPEVVAEGLKVLDAAAQRFGFTLAKTSFD
                     FGGDRYLRTGETLPEGAVDDLRRFDAIYLGAIGHPDVKPGILEKGILLALRFALDQYI
                     NLRPVKLYPNVDTPLKDKGPEDIDFVVVRENTEGLYCGAGGFLKKGTPDEVAVQESIN
                     TRKGVERCLRYAFEYTRQRAKRNTLTLCGKTNVLTYAFDLWARAFQEIGERDYPDIKR
                     EYAHVDATCMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGIAAGGNINPEGVSM
                     FEPIGGSAPKYTGQGVINPLAAIAAGQMMLETLGEKEAAAKVEQGIVHTLSKLKSLSA
                     GKMGYSTSEVGDMVATHVASS"
     gene            3954..>5533
                     /locus_tag="PLY56_05700"
     CDS             3954..>5533
                     /locus_tag="PLY56_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21007.1"
                     /translation="MFQIGVFWEEGFPSADTSPLALNGIRRALDGFDVHVVGYSELER
                     IPSVDLLILPFGSAFPALGWPLLQVYLHSGGNLLNLGGAPFWVPCAREHVLWKRQARQ
                     SSYHRAIGIRHYHVVDPSRIALLDVPPEEPLLTGVREPVRARAIFAPVTSLAGEGAAG
                     AGERVLRPLLYGLDTSGRPVAAPVFALDHIGGPFHGGRWVFAALDGAIPLDMIRRLAF
                     YAAQGASDLAVSASFPCYYPGEVPRLQVHASLFGNVPGIQEASVTVIAPDGKRATCEL
                     PPIGFPDRPAYRWSECAAPLFDQAPAPGLHVIEASLTSQELREFAARLRLPAAARAEF
                     RHHRSGFYVWDPRQATGEPTLTVGRDYLRCEGLSGPVIGTVHSSPQGVTSGLTSDPYR
                     WDSELRLMRKAGIRVVRVNLRAIWARVGLNCPPVEESLLRAVDAFVAAARAHGLLVIL
                     APLEAEGDTPDDAGDLVLESLMGLSTRYHPLARTSPNIVDSLRLLAGRLTAAEVAAAG
                     RANQQPAHPPLGQLVDEYV"
BASE COUNT          889 a         1782 c         1803 g         1059 t
ORIGIN      
        1 cagcaagacg actcttccga gccaatcgcc ctgctcgccg atgttgtggt ccggaacggc
       61 gaagccaagg cattgccggc cggtggagag gatgcgatcc cacatgtggg tcgagtagcc
      121 gggaacgggc tcaccttcct ccaaggggta gcggccttcg cggatgcccc tggcaacgcc
      181 gtcattgtag atctcgatgc ccaggacgca cgggctgaaa tcgagcatct ccagcaccgc
      241 cgcgtcggtc aggtggctcc aggtcgggtg ggcgatgacg atcccgccgc catctgggta
      301 ctccaggtgc tcgatcatct cccggaagcc ctcctgccag gggccggcat acccggtggg
      361 gtagccgtgg tcgcgcaatc ggaagcggtt gcgggcgtcg aagtttccag agcaaaatgc
      421 cgagccaggg caggtgatgt ggcaggggga gttggtaaag gagtggtgct ccgcgttctt
      481 gctgaggagg atatccgccg gcagatcggg aaagagcacg tcggtttcct ggaaggggag
      541 ttgctggcgg tactcctcct cgagctcgtc ctgccacgtg atgagctcgt tccagttgat
      601 gggcgccgga acgggaacgc cgttgcgggt ggtgctccag gcctggcgca gccggaaatc
      661 gctcaagcgc gtgctgggcg tgcaaggcgc cgagggatag tagttggaga tcggaaagtg
      721 tctcaggcca tgccggcgcg cgttttccag gcgccggcgc ggcgacagcg gcgggttttc
      781 ccagggcgct gccaggtgca tgtgcggggt cgaggcgacc cggatcacgt gctgccaatc
      841 gagtgattga tacgggttgt tcatgttcca tctccatcca gcgggcgccg atctcttcgc
      901 ccacgctcgc gagggcatgg ggggagagag gcacggggtc agcgccggtg ctggcacctc
      961 acgcgtcctg ctcgtgggct tcggcggcac gccacagacc tcctcctgga acttccgata
     1021 atgtctctta tgttgacttt gccctcgggg ggggctgtgg cctgaacgat gggggtagcc
     1081 ccgccggtcg agcgagcgcg agggcggttt gagagcggtt gttcccgcgc tcacggaggc
     1141 gaggaagatc gcccgcgcgt gcccttgcca aaatggcgga gaatagtgta gaatggtgcc
     1201 atcagatgtg gatggaaatg cctattgacc gcgaccatac agaccacact tcgcttctgt
     1261 ttcgtctgca ccggccggct cggcgggacg caagcgcccg aggcgatgtg aatggcggga
     1321 ggctcccggc atgacgaagc cgcaggtgaa tctgcaggat gcctttctca accaggtgcg
     1381 caaggagaac acgggggtgg tgatctacct catcaacggc gcgcagatca aaggcatggt
     1441 gcgcggtttc gaccagttta cagtcgtcct ggagagccag ggccggctgc agcttgtcta
     1501 taagcatgcc atcgcctctg ttgtgcccac caagccgatc cccgcgatga atagcgagtc
     1561 ggcatcccga gagcgagccc acatccaatg aacttcctga agtaccacgg tatcggcaac
     1621 gacttcatcc tggtagacgg ctttaaagag gagcttcccg aggagctcct ctccccacag
     1681 gcccagcgcc tatgcgaccg ccacttcggc gtgggcggcg atggtctcat cctcgtcctc
     1741 cccagccgcg tggcggactt ccggatgcgc atgttcaacc cggatggcag cgagtcggag
     1801 atgtgcggca acggcatccg ctgttttgcc aagtaccttt acgactatgg cctcacccgc
     1861 gagacccagg tgaccgttga gacgctcgcc ggcatcatcg ctccaaagat gatcgtcacg
     1921 gatggcaagg tccagatggt ctgtgtggat atgggtgagc cgcgcctggc ccgccaggag
     1981 atccccatgg agggtggcga tgccggaagc cgggtgatcg ccgagaagat cgacgtggat
     2041 ggcgagcgct tcgaggtcac cgcggtttcg atgggcaacc cgcattgtgt cacctttgtg
     2101 gaggacgtcg agcacttccc tgtggctaag atcggcccgc tcatcgagcg gcatcccctc
     2161 ttcccgcggc gaacgaacgt ggagtttatc caggtgctga gcaacgagga gctccagatg
     2221 cgcgtctggg agcggggcgc cggcgagacg ctggcgtgtg gtacgggggc gtgcgcgtct
     2281 gcggtcgcgg cggcgctcaa ccaccgcacc ggtcggaagg tgaccattca cctgcccggc
     2341 ggagacctgc agatcgaatg ggcaggcgac aaccacgttt acatgaccgg cccggcggaa
     2401 gaagtcttta gcggtaccat cgcgctctaa agtactcagg gctagaagca tttagggccc
     2461 gggagagaag atcacttcac ttcacttccc gggccctttc tacgtctgct tcagtgcgcg
     2521 gctgttagct gcttgccacg tgggtggcga ccatatcgcc gacctccgag gtggagtagc
     2581 ccatcttgcc cgcggagagg ctcttcagct tcgacaaggt gtggacgatc ccctgctcca
     2641 ccttcgccgc ggcttccttc tcgccaagcg tctcgagcat catctgtccg gcggcgatag
     2701 ccgcgagcgg gttgatcacc ccctgccccg tgtacttggg cgccgagccg ccgatcggct
     2761 cgaacatgga gacgccttcc ggattgatgt tgccccctgc ggcgattccc atgccgccct
     2821 ggatcatcgc gccaaggtcg gtgatgatat cgccgaacat gttgtcggtg acgatcacgt
     2881 cgaaccactc cgggttcttg accatccaca tgcaggtggc atccacgtgc gcgtactcgc
     2941 gcttgatgtc gggatagtcg cgctcgccga tctcttgaaa cgcgcgtgcc cacaggtcaa
     3001 acgcgtacgt cagcacgttc gtcttcccgc acagggtgag cgtgttccgc ttggctcgct
     3061 ggcgggtgta ctcaaacgcg tagcggaggc agcgctcgac acccttgcgc gtgttgatcg
     3121 attcctggac ggcgacctcg tcaggggtgc ccttcttgag gaagccgccg gccccgcaat
     3181 agagaccctc ggtattctcg cgaacgacca cgaaatcgat atcctcgggg cccttgtcct
     3241 tcagcggggt gtccacattc ggatagagct tgaccggacg caggttgata tactggtcca
     3301 aggcaaaccg gagcgcgagg aggatgccct tctccaggat gcccggcttc acatccggat
     3361 gcccaatagc ccccaggtag attgcgtcga agcgacgcag gtcgtccacg gcgccctcgg
     3421 ggagcgtctc tcccgtgcgc aggtagcggt cgccgccaaa atcaaaagaa gttttcgcca
     3481 gggtgaaacc aaagcgctgt gccgcggcat cgagtacttt caggccctcc gccacgacct
     3541 cggggcccgt gccgtcgccg gggattacgg caatgttgta agtcttggcc acaatccaac
     3601 ctcctcttgg gatctctcgc gggcatcatt gagggcacgc cactggcccc ggggatctcc
     3661 cacggctagc tcgcccctcc cggatgcgcg ggcgcctgcc cttgctccct gacacccgtc
     3721 tctctgattc cccgccgggt agcgaactcc tttgccttcg gaatctgcgg taacggcgcc
     3781 ggcgtggaga gagccttgcc tttcttccgg ggctgtgcta cacttgcggt gggcctgagc
     3841 gtgccccacg tgctgctggt ctcttcgagg ccgcatcatc ctggcttccc cggtctcccc
     3901 gggtcgatcg gggcgtagcc tcacgcgcgt tgcgatacac ctagaaggaa tcaatgtttc
     3961 agataggcgt tttttgggag gaggggttcc cctccgcaga tacatcaccc ctggcgctga
     4021 acgggatccg gcgggcgctg gacgggtttg acgtccatgt cgtgggctac tcagagctcg
     4081 agcgcatccc gagcgttgac ctcctcatcc tgccgttcgg ttccgcgttt ccggccctcg
     4141 gctggccgct tcttcaggtg tacctgcact cgggtggcaa cctattgaac cttggaggcg
     4201 cgcccttctg ggtgccttgc gcgcgcgagc acgtcctctg gaagcgacag gcgcggcaga
     4261 gctcctacca ccgggcgatc ggcatccgcc attatcacgt cgtggaccct tctcgcatcg
     4321 cgttgctgga tgtgcccccg gaagagccgc tcctgactgg cgtgcgcgag ccggtacgcg
     4381 cgcgcgcgat cttcgcgccg gtgacatccc tggccggcga gggcgcggcc ggcgccggcg
     4441 agagggttct ccgcccgctt ctctatggct tggatacctc cggacgcccg gtcgccgcgc
     4501 cagtcttcgc cctagaccat atcggcgggc ccttccacgg cgggcggtgg gtctttgccg
     4561 ccctggatgg cgctattccg ctcgatatga tccgccggct cgcgttttac gcggcccaag
     4621 gcgcctccga tctcgctgtg agcgcttcct tcccctgcta ctatcccggt gaggtgcccc
     4681 ggctccaggt gcacgcctct ctcttcggca acgtgccggg catccaggaa gcgagtgtca
     4741 cggtcatcgc cccagatggg aaacgcgcga cgtgcgagct tccccccatc gggtttcccg
     4801 accggccggc ttatcgatgg agcgagtgcg ctgcgcctct ctttgatcag gcgcccgctc
     4861 cagggctcca cgtcatcgag gcgtcgctga ccagccaaga gctgcgcgag tttgccgccc
     4921 gactgcgcct ccctgcggcg gctcgggcag agttccgcca ccaccgcagc gggttctacg
     4981 tctgggatcc caggcaggcc accggagagc ccactctcac tgttggacgc gactacttgc
     5041 gctgcgaagg tctctcgggg ccggtgatcg gcaccgtcca ctcgtctccg cagggtgtga
     5101 cctccggctt gacgagcgat ccttatcggt gggacagcga actccgcctc atgcgcaagg
     5161 ctggcatccg ggtggtgcgc gtaaacctgc gcgccatttg ggcccgagtg ggcctgaact
     5221 gccccccggt tgaggagagc ctcctccggg ccgtggatgc gttcgtcgcc gccgcgcgcg
     5281 cgcatggtct tctcgtcatt cttgccccgc tcgaggccga aggcgacacg ccggatgacg
     5341 cgggcgacct ggtgctggag tcgctgatgg ggctctccac ccgctaccat ccgctggcgc
     5401 gcaccagccc gaacatcgtc gacagcctgc gcctcctcgc gggccgcctg acggcggccg
     5461 aggtcgcggc ggcgggccgg gcgaaccagc agcccgcgca tccgccgctc gggcagttgg
     5521 tcgacgagta tgt
//
LOCUS       DAOSCV010000174         5530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9271_length_5530_cov_3.181187, whole genome shotgun sequence.
ACCESSION   DAOSCV010000174 DAOSCV010000000
VERSION     DAOSCV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9271_length_5530_cov_3.181187"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLY56_05705"
     CDS             complement(<1..357)
                     /locus_tag="PLY56_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOJ21008.1"
                     /translation="MIGIVTARGDEDRCSRELREAAERQGGGQYIDPLSLIIEVEGSA
                     RLLTSGTPFEGFDAVILRGLNRDGDIDMQFEVFELLETLGIPVLNPASALSAAESKPV
                     TLFRLHRAGLPIPRTLV"
     gene            complement(405..1064)
                     /locus_tag="PLY56_05710"
     CDS             complement(405..1064)
                     /locus_tag="PLY56_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ21009.1"
                     /translation="MPSGAYYLRNLGLLEGLPEGACRLLEQQWRVHHYGRRETIVEQG
                     APADQVHLLAEGSAELVRREQEKETILVVLSPGEFIMGPVEDATAGSLVCARALDECA
                     VCTIGQRELEAIIQMYPMLAWRLLTWSSRNTEQAYSRIQSLASCEVTDKLLALIRHLA
                     GRHGVPTADGLEITLRLTHHDLAALIGSCREAVSRGLKALRDRGVIAVNARCITLRES
                     G"
     gene            complement(1316..2194)
                     /gene="ispH"
                     /locus_tag="PLY56_05715"
     CDS             complement(1316..2194)
                     /gene="ispH"
                     /locus_tag="PLY56_05715"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOJ21010.1"
                     /translation="MKVIVAEQIGFCFGVKRAINLAKKVLSERGEVCILGDLVHNRRV
                     SRELEQMGLRKVSNLSECSSGIMLVRAHGLPAAEIERARAAGLEVIDATCPTVRQAQR
                     AARRLEERGCQVVIAGDADHPEVRGILGSLRDPSAHLVLDSVEEVRQAHAERRFRRPV
                     GVVFQTTHSFERCHALVGELMLVIPELQIINTLCRPVRNRQLDAIRLATEVEVMLVVG
                     SFKSANSIELTHLLRAHNPNTIQVENADQLELARFAGVKSVGIASGLSTPEDVVDAVR
                     ARLEAAFPEPDSSPAL"
     gene            2372..3376
                     /locus_tag="PLY56_05720"
     CDS             2372..3376
                     /locus_tag="PLY56_05720"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ21011.1"
                     /translation="MVTIALAGDVMLGRGVNEVLGKMRPEEPWGDVLPLMTGGHGYPE
                     VDLRIINLECALTTRLQPWTRTPKVFHFRADPSVVSVLQAARIDACSLANNHTLDYEE
                     EGLLDTLRHLDEAGIQHAGAGKDERHAARPVILRAGQEGTRVGLVAVTDNEPAFAAGP
                     DRAGTHYLPVSMEIEVLRRVEDAVGMARAAGAEIVVFSNHWGPNMVMHPSRLFQEFAH
                     AVIDRGADLYYGHSAHLFQGVEIYRGKPILYDTGDFIDDYAVDPVLRNDWSFLFVATL
                     ERGSFRRLEMHPVVLSYARVSRATGTEREAILERMEHLSAELGTSLLRAEEHLVAEPG
                     "
     gene            complement(3455..4354)
                     /locus_tag="PLY56_05725"
     CDS             complement(3455..4354)
                     /locus_tag="PLY56_05725"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOJ21012.1"
                     /translation="MEKNPFRSPGRWFRGNTHTHSTRSDGRVSVAGRFEAYREKGYDF
                     LVLTDHGKVSDISAFTDNTFLAISGSELHPENPYGGATYHLVALDLHEPIDVAGMHPN
                     EVIQAVHAQGGHVIIAHPYWCGHTLFDLLPLEGGFAVEVYNDTCAGIGKEISEVQWDD
                     LLDRGKRLLGIACDDAHGTEHDCFHGWVMVKSEELTREAILEALLKGCYYSTTGPEIL
                     DLDLVETERDGQPARKVVVRTSPARSIVFKAQRYFGRRCLPPEGGLLEYAEYTLHGSE
                     RYVRVEVTDAEGRKAWTNPFFLD"
     gene            complement(4401..5234)
                     /locus_tag="PLY56_05730"
     CDS             complement(4401..5234)
                     /locus_tag="PLY56_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ21013.1"
                     /translation="MTIWWYTLGSTFLISLIALVGILSLYLSADRVREWLLPMVGFAA
                     GALLGNVFLHLLPELVERKGSFDLASSLLVLLGIVTFFLMEQGIRHWQAEHTGREDHI
                     EHLAHLRPRTVDLEQGAGHLLSARPYAAVSLGSDLLHNFIDGLVVGGAFLFSIPLGIS
                     TALVVALHEVPQELGDYAILVHAGLDRVRALLYNFVTALTAVAGGVVALLIGAARETF
                     PLYLIPFTAGTFIYLAVGSLIPELHSHGEEHRTIRGVVGMLVGIGVMVVTHVAAHQVG
                     H"
BASE COUNT          995 a         1881 c         1767 g          887 t
ORIGIN      
        1 caccagggtc cgaggaatag ggaggccggc ccgatggagt cggaagaggg ttaccggctt
       61 actctctgcg gcggagagcg ccgatgccgg attgaggacg gggataccga gcgtctccaa
      121 cagctcgaac acctcgaact gcatgtcgat atcgccgtcg cggttgaggc ctctcaggat
      181 aaccgcgtcg aagccttcaa acggggtgcc actggtcaga aggcgcgcac tcccctccac
      241 ctcgatgatg agcgagagcg gatcgatgta ttggcccccg ccttgccgct ctgccgcctc
      301 gcgaagctct cggctgcaac gatcctcatc cccccgagcg gtcacgatgc ctatcaccgc
      361 atcctccaat caggagcgca ccggcagccc gcgccattcc ccggtcatcc agactcgcga
      421 agggtgatgc agcgcgcatt gactgcgatc accccgcggt cgcgcagggc tttcaggcca
      481 cgggagactg cctcgcggca actcccgatg agcgccgcca ggtcgtgatg ggtcagtcgc
      541 aacgtgatct caaggccatc agccgtcggc acgccatgcc ggcccgccag gtgccggatc
      601 aaggccagga gcttatcggt cacctcgcaa gaggcgagcg attgaatgcg cgagtaggcc
      661 tgctccgtgt tccggctcga ccaggtgagg aggcgccagg ccagcatggg gtacatctgg
      721 atgatcgcct ccaactccct ctgacctatc gtgcacacgg cgcactcatc cagcgcccga
      781 gcacacacga gggacccagc tgtcgcatcc tccacggggc ccataatgaa ctcgccggga
      841 gacagcacca ccaggatcgt ctccttctcc tgctcccgcc gcaccaactc cgccgagccc
      901 tcggcaagaa ggtgtacctg gtccgccggc gccccctgct ccacgatggt ctcgcgccgc
      961 ccgtagtggt gcacgcgcca ttgctgttca agcaagcggc aggccccctc gggaaggccc
     1021 tccaacagcc ccaggttcct caggtagtag gctccagacg gcatgccctc tcccgatgcc
     1081 gctctctcac cccggatcac acacccattc catcagggaa agcgggggcg ccagcctcgc
     1141 cggattccct gacctcgcga gcgggctggg catccgcgat agcctgcccg cctgcccatc
     1201 ggcggatcta tacccgacag gggatgcacc aaaacgcggg cgcgcgccag cgagaggcaa
     1261 cgccggtctg gtggcgcgtg gttccgcagg agaggagtgg ccgcagtgga acgccctaga
     1321 gcgccggact cgaatccggc tccgggaagg cggcctcgag ccgcgcacgc accgcgtcca
     1381 ccacgtcctc cggggtagag agcccagacg caatgccgac actcttgacc ccggcgaacc
     1441 gggccagctc cagctggtcc gcgttctcga cctggattgt gtttgggttg tgcgcgcgaa
     1501 ggaggtgagt tagctcaatg gagttggcgc tcttgaagct gccgacgacc agcataactt
     1561 ccacttcggt cgccaggcga atggcatcga gctgccggtt gcggaccggc cggcagaggg
     1621 tgttgatgat ctgcaactcc gggatgacca gcatcagctc gccgacgagg gcgtggcagc
     1681 gctcgaacga gtgggtggtc tggaacacga ctcccaccgg gcggcggaac cgccgctccg
     1741 cgtgggcctg ccgcacctcc tcgaccgaat cgaggaccag atgagccgac ggatcgcgga
     1801 gactgccaag gatgccgcgc acctccgggt gatcggcgtc gccggcaatc accacctggc
     1861 agccacgttc ctccaggcgg cgggccgccc gctgcgcctg ccggaccgtt gggcaggtgg
     1921 catcgatcac ctccagcccg gccgcgcgcg cgcgctcgat ctccgccgcg ggaagcccgt
     1981 gcgctcgcac cagcatgatg ccgctgctgc attcgctgag gttgctcacc ttccgcaacc
     2041 ccatctgctc cagctcgcgg ctcaccctgc ggttgtggac cagatccccg aggatacaca
     2101 cctctcctcg ctcggagagg accttcttgg ccaggttgat ggctcgcttg acgccgaaac
     2161 aaaacccgat ctgctcggcc actatcactt tcatcggtcc ctagttctcg cagccccacg
     2221 ggcttccctg ctggcgacag gcgcctttct gccccgcggc gggcggccag gaggctgccg
     2281 ccctgagcta agggctatct ggacaagcgc ggcgcgctgc ccgttttgac cggggcggcg
     2341 cgggcaacca tcgcgcggga gggaacgaga agtggtcacc atcgcgctgg cgggggatgt
     2401 gatgctgggc cggggcgtga acgaggtgct cgggaagatg cgccccgagg agccctgggg
     2461 cgacgtgctc ccgctgatga ccggcgggca cggctatccg gaggtggacc tgcgcatcat
     2521 caacctggag tgcgcgctca cgacacggct tcaaccctgg acgagaacgc ccaaggtgtt
     2581 ccacttccgc gcggatccca gcgtggtctc ggtgctacag gcggcgcgga tcgatgcctg
     2641 ctccctggcg aataaccaca ccctggacta tgaggaagag ggcctgctcg acaccctgcg
     2701 ccacctggac gaggccggga tacaacacgc cggcgccggc aaagatgagc gacatgccgc
     2761 tcgcccggtg atcctgcgcg ccgggcagga gggcactcgc gtcgggctcg tggcggtcac
     2821 cgacaatgag cccgctttcg cggcgggccc ggaccgggct ggcacgcact acctaccagt
     2881 ttcgatggag atagaggtcc tccggcgcgt tgaggatgcc gtggggatgg cacgcgccgc
     2941 gggcgccgag atcgtggtct tttcgaacca ctggggtccg aacatggtga tgcaccccag
     3001 ccgcctcttc caggagttcg cgcatgccgt gatcgaccgg ggcgcggatc tctactacgg
     3061 acacagcgcc cacctcttcc agggggtcga gatctaccgc ggcaagccga tcctctacga
     3121 cacgggggat ttcatcgacg actacgccgt ggacccggtg ctgcgcaatg attggtcctt
     3181 cctcttcgtg gccaccctgg agcgcggcag cttccggcgg ctcgagatgc acccggtggt
     3241 tctctcctac gcgcgcgtct cccgcgccac gggcacggaa cgcgaggcga tcctggagcg
     3301 gatggagcac ctcagcgccg aactgggcac atcccttctc cgcgcggagg agcacctcgt
     3361 cgcagaaccc ggataagcgc cccaaggcgc ggcgtgccct cagtcgcgcg ggctccgggc
     3421 attccgcttg cccactgcgc tcaccctggc ggcgtcaatc caggaagaag gggttcgtcc
     3481 acgctttccg gccttcggca tcggtcactt ccacgcgcac gtagcgctcc gacccgtgga
     3541 gcgtgtactc ggcatactcc agcagaccgc cttccggagg caggcagcgc cggccaaagt
     3601 agcgctgcgc cttgaagacg atggagcgcg ccggcgaggt gcgcactacc actttccgtg
     3661 ccggctgccc atcgcgctcg gtctccacca agtcgaggtc gaggatttct ggcccggtag
     3721 tcgagtagta gcagcccttc aggagagctt ccaggatcgc ctctcgggtt agctcctcgc
     3781 ttttcaccat cacccagccg tggaagcagt cgtgctccgt gccatgcgcg tcatcgcagg
     3841 cgatgccgag caggcgcttg ccgcgatcca ggaggtcgtc ccactgcacc tcggagatct
     3901 ccttcccgat cccggcacac gtgtcgttgt acacctcgac ggcgaagcca ccctccagcg
     3961 gcaagaggtc gaagagcgtg tgaccgcacc agtacggatg agcgatgatc acgtgaccgc
     4021 cctgggcatg gaccgcctgt atcacctcgt tggggtgcat gccggcaaca tcaatcggct
     4081 cgtggagatc gagggcgacc aggtgatagg tggcgccccc gtaggggttc tctggatgca
     4141 gctcgcttcc cgagatggcg aggaaggtgt tatcggtgaa ggcagagata tcgctcacct
     4201 tcccatgatc ggtgagcacc agaaagtcgt agcccttctc acgataggcc tcaaaacgcc
     4261 cggccaccga gacgcggccg tcggatcgcg tgctgtgcgt gtgcgtattg ccccgaaacc
     4321 accgcccggg agatcggaat gggttcttct ccatgttcct gatgccacct tccaccacgt
     4381 gcccggtgaa cacccgtcgc tcaatggccc acttgatgag cggcgacgtg cgtcacgacc
     4441 ataaccccaa tcccgacgag catcccgacc acccctcgga tggtgcggtg ctcctcgcca
     4501 tgcgagtgta gctccggaat gaggctgccc acggccagat agatgaaagt gcccgctgtg
     4561 aatgggatga gatagagcgg aaacgtctcc ctcgcagcgc cgatcaggag ggccaccact
     4621 ccgccggcga ccgcggtgag cgcggtgaca aagttgtaga ggagcgcgcg cacgcggtcg
     4681 agccccgcat gcaccagaat ggcataatcc cccagctcct gaggcacctc gtggagcgcc
     4741 accacgagtg ccgtggagat cccgagtggg atcgagaaga gaaacgcccc gccaacaacg
     4801 agaccgtcga tgaagttatg gagcaggtcg ctacccaggc tgacggcggc atagggccgg
     4861 gccgacagaa gatgcccggc gccttgctcg agatccacgg tgcggggccg caggtgagcg
     4921 agatgctcga tgtggtcttc gcggccggtg tgttcggcct gccagtgccg gatcccctgc
     4981 tccatcagga agaaggtgac gatcccgagc aacaccagga gcgaggaggc cagatcgaac
     5041 gagcctttgc gctcaaccag ctctggaagc aggtggagaa agacgttgcc aagcagcgcc
     5101 cctgcggcaa acccgaccat gggcagcagc cactcgcgca cccggtccgc gctcaaatag
     5161 aggctgagga tgcctacgag agcaatcagg gagatgagaa acgtgctccc cagcgtatac
     5221 caccagattg tcatcaagtg ctcccccgta gccatccatc gccttctatc gcgttcactc
     5281 tatcgaattc gccgccgcga ggggcaaccc cttcccccta gagggcttgt tgtgcggcct
     5341 gcacaggcgt aaggatccgc gccgcgtccc ggctggaggc ctcaagatcg cgcgggcaca
     5401 aatacccacg cgcctggcag cttagggcga agccggctct gtcaccgggg cgagatgcgg
     5461 acgtagaagt tgcctggcag gcggcgggca gcgcccgcca tctccaggag ggtgagcgcg
     5521 gagaggacct
//
LOCUS       DAOSCV010000175         5514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9313_length_5514_cov_3.272944, whole genome shotgun sequence.
ACCESSION   DAOSCV010000175 DAOSCV010000000
VERSION     DAOSCV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9313_length_5514_cov_3.272944"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLY56_05735"
     CDS             <1..288
                     /locus_tag="PLY56_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21014.1"
                     /translation="IPFVDDLNGPVLCEFKPSFLRGARVMVGHSILLRYAGVSYISYE
                     ADIAEKNAFFESVGALPPLTAPVAARLAEMGEEKAYAAIRDAAARGAWPGA"
     gene            complement(365..1003)
                     /locus_tag="PLY56_05740"
     CDS             complement(365..1003)
                     /locus_tag="PLY56_05740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ21015.1"
                     /translation="MAAGNALLIVEFAVVFVGVPALLTTYRLPVPKILLLLAVAGACL
                     TWLLRAPGFDRKVLWNTEGFRRGARGVLRLFAVTAPLLVAGVLVFDPESWLGLARRSP
                     IIWLLVMVLYPLLSVYPQELIYRAFLFHRYRPLLRTEAMLTWASALSFAWMHIIYGNA
                     VALLLSGCGGFLFATTYRRSGSLLLASFQHALYGCLIFTIGLGRHFYRGPAF"
     gene            complement(1336..1533)
                     /locus_tag="PLY56_05745"
     CDS             complement(1336..1533)
                     /locus_tag="PLY56_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21016.1"
                     /translation="MLARMHLSGACQMMAHGKPLYAHELALCGLALLANAPQTHACRD
                     LREQLLTLLQEITRERPPRGM"
     gene            complement(1717..1932)
                     /locus_tag="PLY56_05750"
     CDS             complement(1717..1932)
                     /locus_tag="PLY56_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21017.1"
                     /translation="MEEQQSRRLLLWGTLLLLNGLVIAGYGLWQYVQANPGTPAWVGA
                     WTGLIAGHLLAIAGILTALYYFIRRVV"
     gene            complement(2063..2797)
                     /locus_tag="PLY56_05755"
     CDS             complement(2063..2797)
                     /locus_tag="PLY56_05755"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOJ21018.1"
                     /translation="MHPGGGPPIFISGCNRGGTSILSLLLGQHPQLSNVGKGPHHEGQ
                     WIWKRRFYDGSHHRWALPPWLATLRKTEADATPELLAFFRQAFAAACPTGRLLEKTPS
                     NAIRIPFINCLYPDCFVIHVVRDGRHTVASLIARGVPMPHAPCQWVSVHTIALPDLAA
                     LGSKRGLLVRYEELLQAPMEVLTRICQHCALDDGPKAMAALAAGVERHLKQPYDRWRT
                     LDDKTRHKILDVIGDLQSSLRYPVEM"
     gene            complement(3365..5251)
                     /locus_tag="PLY56_05760"
     CDS             complement(3365..5251)
                     /locus_tag="PLY56_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21019.1"
                     /translation="MHLILGTLALLGSVAAPEGFTDHGVAAKVAEGRGVVTTRDSNGR
                     YLAIVMVRDVGSPVGYILFTDIDTGKTKQFHYPQDAANSDIFASLMSRNGRFYTGAGK
                     VLLEFDPTTEKWLYHGVPQPKAQHFVGAAICDGPDGRIYIGTYPNCHLVSYDPETQTI
                     QDHGQLDPKEQYFNYLVFDSKGWAYAGIGTARCNLVAFHPQTGERRQLMPEEERKPGT
                     VSVVAGAGGEAYAYFGGQWYRLFDGKAEKIPVEAVGPRVRTGAISWGNKRGSFPDGRQ
                     VVDYSVPERWVEVADAKGEQKKRIPLEFEAAGADITSLAAGPGGDVYISTCHPMHLAR
                     LETATGTLHDLGPIPRVGGGNFCAMDTLGNQLFGAAYAHGGLWAYDPAQPWKPAQKVS
                     EPGNPRLLAEWQKDVCRPRTALAHPDGKHVLFAGFADYGYTGGGIGIVNVETGEATLL
                     TADKDLLPGHSTITLKALPDGTLVGGTSIQAPGGGHVIAKEPELYLLDWKTKKVIYRA
                     PVVPGGGDIISIHVGPDGLVYGLTANSTFFVFNLRERKVVHQESFAQYGGVPRHALQV
                     GPDGKIYAMLTRAIVRITPGRFAHEKLADAPVTIEAGGALVKGRLYFSSGPRVWSYQV
                     PGLR"
BASE COUNT          960 a         1805 c         1875 g          874 t
ORIGIN      
        1 atcccgttcg tggacgacct gaacggcccc gtgctctgcg agttcaagcc gagctttctg
       61 cggggcgcgc gcgtgatggt cggccactct attctcctgc gctacgcggg ggtctcctac
      121 atctcgtacg aagcggatat cgccgagaag aacgccttct tcgagtcggt cggggcgctg
      181 ccgccgctga ccgcgccggt ggccgcccgg ctggcagaga tgggcgagga gaaagcctac
      241 gccgccatcc gcgatgccgc ggccaggggc gcctggccgg gagcgtaacg cggtctccgc
      301 gccggcctcg cgcgcgagaa gcccgtgaca gctgcaggag caggagcgac cgagccgggc
      361 gcgctcagaa cgcggggccg cggtagaaat gccggcccaa gccaatcgtg aagatgaggc
      421 agccgtagag cgcgtgctgg aacgaggcga gcaacaaaga gcccgagcgg cggtaggtcg
      481 tcgcgaagag aaagccgccg catcccgaga gaagcagggc cacggcgttc ccgtagataa
      541 tgtgcatcca ggcgaacgag agtgcgcttg cccaggtgag catcgcctcg gtccggagga
      601 gcggccggta gcggtggaag aggaacgcgc ggtagatcag ctcctggggg tagaccgaga
      661 ggagcggata gagcaccatc accaggagcc agatgatcgg cgagcgtcgc gccagcccga
      721 gccacgattc cgggtcgaat accaggaccc cggcgacgag caggggggcg gtcacggcga
      781 agagccgcag cacgcccctc gcgccccggc ggaagccctc ggtgttccat agcaccttgc
      841 ggtcgaagcc cggggcgcgg aggagccacg tcaggcaggc gccagcaacg gcgagaagga
      901 gcaggatctt gggcacgggc agccggtagg tagtcagcaa cgccggcacg ccgacgaaga
      961 caaccgcgaa ttcaacgatg agcagggcgt tgcccgctgc cacgatggct cctctccagt
     1021 cgggtgccgc atagttgcgg cactcccttc atccctcctg gcaagtgcag gcgcgcgcga
     1081 gccttggcgt cattccgtgg acagtggtca cgaaccctgg ccggcagaag cgcgcgagcg
     1141 gcaggatggc actggcaaga gattcgatgg cgagcgcgcg gaatcccgcc agggaagggc
     1201 ggtttcccgc ggcaccgaaa gctgcgtttg agccatgcgg cagcgatccc gggcgcgggg
     1261 cgttgccggc gtcgcctcga tgacgggcag accggcgcgc gccatcatga acgcttcgcg
     1321 gagccgagga tggcgtcaca tgcctcgcgg tggcctctcg cgcgtgatct cctgaaggag
     1381 cgtgaggagc tgttcgcgca agtcgcgaca cgcatgcgtt tggggagcgt tggcgagaag
     1441 cgccaggccg cagagcgcca gctcgtgtgc gtagagtggc ttgccatgag ccatcatctg
     1501 gcaggcgcct gacaggtgca ttcgtgcgag catcggaatc tccgcgggcg tgaagggctc
     1561 gtcctcgcgt cgcgcccgtt ccgcgcagtc gcggcatcgg gcccgggggg aatgcacgcg
     1621 catcacagca cctcccaaac agcgagcggc gcgcctgggc ggggggcggc cgcatggagg
     1681 tatgctaccc cgaaacagcc cgtcctaatc ctccgctcat acgacgcgcc ggatgaagta
     1741 gtaaagcgct gtcaggatgc cggcgatggc cagaagatgc ccggcgatga gccctgtcca
     1801 cgcgcccacc catgccggag tgcccgggtt ggcctgaaca tactgccaca ggccgtagcc
     1861 cgcgatcacc aatccgttca acaagagcag cgtgccccag agtaagagcc gcctgctctg
     1921 ttgctcttcc attggcgcct gcccctcccc gtttcgcacg cgagacaacc agtgtcaccc
     1981 tcactctacc acaggagagc tccgtctcaa acactgcttc accgggccca gcgccccggc
     2041 agcacgcgat acgatgggga agctacatct ctaccgggta gcgcagggat gactggagat
     2101 ctccaatcac gtcgaggatc ttatgacgtg tcttgtcatc gagcgtgcgc cagcggtcgt
     2161 agggttgctt gagatggcgc tccacacccg ccgcgagtgc agccatcgcc ttggggccat
     2221 cgtcgagcgc gcagtgctgg cagatgcgcg tcaacacctc catgggcgct tgcagcagct
     2281 cctcgtagcg caccagcagc ccgcgcttgc taccaagcgc cgccaggtcg ggcagggcga
     2341 ttgtgtgtac ggagacccac tggcacggtg cgtgcggcat cgggacgccg cgagcgatga
     2401 gcgacgccac cgtgtgccgg ccatcacgga ccacatggat cacgaagcaa tctgggtaga
     2461 ggcagttgat gaagggaatc cggatggcat tcgagggagt cttctccagc aggcggcccg
     2521 tagggcaagc ggcggcaaac gcctggcgaa agaacgccag cagctccggg gtggcatctg
     2581 cctcggtctt gcgcagagtg gcgagccagg ggggcagcgc ccaccgatgg tggctgccgt
     2641 catagaaccg acgtttccaa atccactggc cctcatgatg ggggcctttg cccacattgc
     2701 tcagctgggg gtgctggccg aggagcagcg acaggatcga ggtgccgccc cgattgcagc
     2761 cggagatgaa gatcggcggc ccgccccccg gatgcaagag gtcgccatct ccgcgcgcgc
     2821 gtccgcgcca ccatcgccag ggggagaacc aggggaatcc tgcaacctgg agaagacctg
     2881 ggcggacagc gagcgcaccc tgcttatcag cgaccagtcg ttcctgcatc ctggggctaa
     2941 gagacaccgt gtgggactcc ctccttctgc aacgacactg ggcgcgacca agcggggctc
     3001 ggtcgtgcca ggtgttccat cgtgcatccg cacgggcatg ccgggccccc atgcctatcc
     3061 ggaagtcgtt cgggtcagga tgcggtgtcc ggacacgagg cgcaagccca ccggctcgcc
     3121 gccagcaggg cgatggcgtg ccacttcgct gggcaggccc ctggtggtgg gaaggggcct
     3181 gcccctcatg agtacccgcc tgcgtcggca ttccagatcg ccgcgcagtg gcgcctgccc
     3241 gcgcctctcg cgtgcggcat agctcccgcg agagaagcac tatatcgcgg ggagtgacca
     3301 gacggcgtga aaccagagcc gacgcgccac ccaggaccat ccaggcatgg gggaggatac
     3361 tctatcagcg taggcccggc acctggtagc tccagacgcg cggcccgctg gagaagtaga
     3421 gccggccttt caccagcgcg ccccccgcct cgatcgtcac cggcgcgtcc gcaagcttct
     3481 cgtgcgcgaa gcggcccggc gtgatccgca cgatcgcgcg cgtgagcatc gcgtagatct
     3541 tgccatccgg tccgacctga agcgcgtgcc ggggcacgcc gccatactgc gcgaagctct
     3601 cctgatgcac cactttccgc tcgcgcaggt tgaacacgaa gaaggtgctg ttcgcggtca
     3661 gcccatacac caggccatcg gggccgacgt gaatgctgat gatatcgcca ccgcccggca
     3721 ccaccggcgc gcggtagatt accttctttg ttttccaatc cagcagatag agctctggct
     3781 ccttcgcgat gacgtggccg ccgccgggcg cctggatgct ggtgccgccc accagggtgc
     3841 catccgggag ggccttgagc gtgatggtgc tatggccggg gagaaggtcc ttgtccgcgg
     3901 tgaggagggt ggcctcgccg gtctctacgt ttacgatgcc gatgccgccg ccggtgtagc
     3961 cgtagtcggc gaagccggcg aacaggacgt gcttgccgtc aggatgggcc agcgccgtgc
     4021 gtggccggca gacgtccttc tgccattccg ccagcagcct ggggtttcca ggctcgctca
     4081 ccttttgggc cggcttccac ggctgagccg ggtcgtaggc ccagaggccg ccgtgggcat
     4141 aggccgctcc gaacagctgg ttgcccagcg tatccatcgc acagaagttc ccgccgccga
     4201 cgcgtgggat cggccccagg tcgtgcagag ttccggtcgc cgtctctagg cgcgccaggt
     4261 gcatcgggtg gcaggtggag atgtagacgt cgccgcccgg accggccgca aggctggtga
     4321 tgtccgcgcc ggccgcctcg aactcgagcg ggatccgctt cttctgctcg cctttggcgt
     4381 ccgccacctc cacccatctt tccggaacgc tgtagtccac cacctgccgg ccatcgggga
     4441 agctccccct cttgttgccc cagctgatcg cgccggtgcg tacccgcggc ccaacggcct
     4501 ccacggggat cttctccgcc ttgccatcga agaggcggta ccactggccg ccgaaatagg
     4561 catacgcctc gccaccggcg ccggcaacca cgctcacggt gcccggcttg cgctcctcct
     4621 ccggcatcag ctggcgcctt tcccccgttt ggggatggaa cgccaccagg ttgcaccgcg
     4681 cggtcccaat tccggcgtag gcccagccct tgctatcgaa aacgagatag ttgaaatatt
     4741 gttccttggg gtcgagctgg ccgtggtctt ggatggtctg ggtttcgggg tcgtaagaga
     4801 cgaggtggca gttggggtag gtgccgatgt agatccgccc gtccgggccg tcgcatatcg
     4861 cggcgccgac aaagtgctgg gccttcggtt ggggcactcc gtggtagagc cacttctcgg
     4921 tggtggggtc aaactcgagc aaaaccttcc ccgccccggt atagaagcgc ccgttccggc
     4981 tcatcagcga ggcgaagatg tccgagttcg cggcgtcttg cgggtagtga aactgcttcg
     5041 tcttcccggt gtcgatatcc gtgaacagga tgtagccgac cgggcttcct acatcgcgaa
     5101 ccataacgat agccaggtag cgcccgttgc tgtcgcgcgt agtcaccacg ccgcgaccct
     5161 ccgctacctt cgccgcgacg ccgtggtcgg tgaacccctc gggtgcggcc accgagccca
     5221 gtagagcaag cgtgcccaag atcaagtgca tgatgtgcct ccggttccgg tcgagtggga
     5281 gccatgggag cgtgcggcct tggcccacgc ctcgccacag gaacggttcg gcataggcag
     5341 ggcacctcct gcctcgcggc gaagctacca ggggaaggag aagagcatgc aggaagtgac
     5401 catgcacccg ggaggaagcg ccgaggtcga gcagcgcgcg ctgcagaccg tggcggatct
     5461 gatctgtttg gcggcgcgca cggcgccaaa ggcaagaggg gccgaccatc ttgt
//
LOCUS       DAOSCV010000176         5512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9319_length_5512_cov_3.283672, whole genome shotgun sequence.
ACCESSION   DAOSCV010000176 DAOSCV010000000
VERSION     DAOSCV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9319_length_5512_cov_3.283672"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1845
                     /locus_tag="PLY56_05765"
     CDS             160..1845
                     /locus_tag="PLY56_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ21020.1"
                     /translation="MRFLRLLPVFLLLATGPACADWQVWTLARTERVLREDPAGTGIA
                     VQLGAARNEWECFQVLMRSDQPVTGIRLEPGDLKGPQGAVLPARDARLYRQHQLEITI
                     GSYRNEAFRPGWYPDPLIPFDHPVTRQPLGEARFKAIPFDLPANETHGFLVDLYVPAN
                     AVPGVYRGDYRLTAPGRPAVTIPVTLTVWKLTLPNVPTLQTALGSPASRLRTHYQQRA
                     KDGKEAEPTDWAAVEEQCAELLSRHRINATPPPGTLAVQAQPDGSFRIPKEQVDRFRE
                     FVDRYHVNAFSVPRPTSVVKDPEAEREKLHAWLKAWDEAARELNRPHVTFYTYLRDEP
                     NDEEAYRFVQKWGRAIRAARSVVKVLVVEQTWTQNEQWGDLYGAVDIWCPLWPLFKEE
                     SARTRQALGETVWCYTALCQREPTPWWLLDTPLLNYRVPTWIAWRYGIRGLLYWGGMA
                     YWQQVDDPWTDPKTLDRRNRHPEGKGPLYNGEGSLLYPGRAVGYDGIVESLRVKALRD
                     SIEDYEYLAQLEAAGKRAQAEEVVRPLAGSWFEWEKDPAAYEKARIALARLME"
     gene            1869..3287
                     /locus_tag="PLY56_05770"
     CDS             1869..3287
                     /locus_tag="PLY56_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21021.1"
                     /translation="METPTKKSRFALFFGNRGFFPGSLMASAREELTRVLSTMGHETL
                     VLDASATRYGAVETPAEGEIYARFLKENEGKFDGVILSLPNFGDENGAVAALREAGVP
                     ILIQAYPDEMDKMAPATRRDAFCGKLSIMDVFCQNGIPFTALKPHTVSPASPRFAQNV
                     EYFDRVCRVVKGMRNLVVGAVGARTTAFKTVRIDEVALQRHRITMETFDLSDLFARMR
                     AVDPNSDAYRAKEDRYLGYADFSSVPGDKRDTLVRLGVALDTLIEEARLDTIALRCWE
                     EMQRQLGISPCVVLSEMNNRGITAACEVDVGNAIAMHALGMASGEVTACLDWNNNYGD
                     DDDKCILFHCGPVPQSMMTAKGKVEEHAILANAVGRSCSIGCNVGRIAPTSFTFASMT
                     TDSGRLKFYVGEGEFTNDPIPPEFFGCAGVARVPALQDVLLHVGRCGHRHHVSVTPGQ
                     VADPVCEALTNYLGYDVARPQP"
     gene            3583..4233
                     /locus_tag="PLY56_05775"
     CDS             3583..4233
                     /locus_tag="PLY56_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21022.1"
                     /translation="MQSTRRGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCT
                     SNGRQLGTALMQYCQDYDETLPYGQHEAPDSRFSFQVLLPYMKNEEIFACPSDKNVVT
                     SFVADGYGSARTIPHGWGVDQYHYPYRTIYSQPVTLANISRVSESAVFFEKTNAVTTM
                     PYVYCPIHNAPLSTQVAGRHNDGLIVVFYDGHAKWMKREEVIGGPNVARLWLHDNS"
     assembly_gap    4655..4754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4755..5311
                     /locus_tag="PLY56_05780"
     CDS             <4755..5311
                     /locus_tag="PLY56_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21023.1"
                     /translation="RARENARKSTCTSNGRQLGTALMQYCQDYDETLPYGHHEAPDSR
                     YSFQCLLPYMKNESIFACPSDKNVVSTFVADGYGPARTIPHAWGVCQHHYPYRVIYQQ
                     PMTLGQISRPSESGVFFEKTNVVTTMPYVYCPIHNPANVTQVADRHMDGLIIVFFDGH
                     AKWMKKDEVIGGPNVARLWLHDNS"
BASE COUNT         1009 a         1802 c         1706 g          895 t
ORIGIN      
        1 cacgcgggcg cgggtctgac gcccctacca cacgttcgct cgcacatcgc gttgcccgca
       61 ccatccgagc ggtagatgca gcaccgggat gcggctcgcg caaggaggag ttggcgcgcg
      121 gagggctaat gcagaatgca acacaaaagg agggtagcga tgcgattcct caggttactc
      181 ccggttttcc ttttactagc caccggaccg gcatgcgccg attggcaggt gtggacgctt
      241 gccagaacag agcgcgtgct gcgcgaggat ccggcaggca ccgggatcgc cgtgcagctt
      301 ggcgcggcgc gcaacgagtg ggagtgtttc caggtgctga tgcgctcaga tcagccagtc
      361 actggcatcc gcctggagcc cggcgatctg aaggggcccc agggagccgt gcttccggca
      421 cgcgatgcac gcctctaccg ccagcaccag ctcgagatca ccatcgggag ctaccggaac
      481 gaggcgttcc gccccggatg gtatcccgac ccgctcatcc ccttcgacca cccggtgacg
      541 cgccaacccc tgggtgaagc ccgtttcaag gcgatcccct tcgacctgcc agcgaacgag
      601 actcacggct tcctggtgga cctctatgtc ccagcgaacg ccgtgccggg cgtgtatcgc
      661 ggcgactacc ggctgaccgc cccgggccgg ccggccgtca ccattccagt cacgctcacc
      721 gtctggaagc tcacgctgcc caacgtcccg acgttgcaga cggccctggg cagcccggca
      781 tcgcgcctgc gcacccacta ccagcagcgc gcgaaggatg gaaaggaggc cgagcccacc
      841 gattgggcag cggttgagga gcaatgcgcg gagttgctct cgcgccaccg gatcaacgcc
      901 acgccccctc cgggcaccct cgccgtccag gcacaaccgg atggctcctt ccgcatcccc
      961 aaggagcagg tggaccggtt ccgcgagttc gttgaccgct accatgtgaa cgccttcagc
     1021 gtgccccggc ccacttcggt ggtgaaggac cccgaggcgg agcgcgagaa gctgcacgcc
     1081 tggctgaagg cctgggacga ggcagcccga gagctgaacc gcccccacgt taccttctac
     1141 acctacctgc gcgacgagcc aaacgatgag gaggcctatc gcttcgtgca gaagtggggc
     1201 cgcgccattc gcgccgcgcg ctccgtggtg aaggtgctcg tcgtggagca aacatggact
     1261 cagaacgagc aatggggtga cctctacggg gccgtggaca tctggtgccc tctgtggccg
     1321 ctcttcaagg aggagagcgc gaggacgcgc caggcgctgg gcgagacggt ctggtgctat
     1381 acggcgctct gccagcgcga gccgactccc tggtggctgc tcgacacccc gctcctcaac
     1441 taccgcgtgc ccacctggat tgcctggcgg tatggcatcc gcggcctcct ctattggggc
     1501 ggaatggcct actggcagca ggtggatgac ccgtggaccg atccgaaaac gctcgaccgg
     1561 cgcaaccggc acccggaagg caagggcccg ctctataacg gtgagggcag cctgctctac
     1621 cccgggcgcg ccgtgggcta cgatggcatc gtggaaagcc tccgcgtcaa agcgctgcgc
     1681 gactcgattg aagactacga gtacctcgcc cagctcgaag ccgcgggaaa acgggcgcaa
     1741 gccgaggagg tggtccgccc cctcgccggc tcgtggttcg aatgggagaa ggatccagcc
     1801 gcctatgaga aggcgcgcat cgctctggcg cgtctgatgg agtagaccat gcgaagggag
     1861 accgcgagat ggagactcca acgaagaaat cgcgctttgc tctcttcttc ggcaaccggg
     1921 ggttcttccc cggctctctg atggcgtcgg cgcgtgagga gctgactcgc gtgctgagta
     1981 ctatggggca cgaaacgctc gtgctggatg cttcggctac gcgctatggc gcggtggaga
     2041 caccggcgga gggagagatc tacgcccggt tcctcaaaga gaacgagggt aagttcgacg
     2101 gcgtcatcct ctccctcccc aatttcggcg acgagaacgg cgccgtcgcg gcgttgcgcg
     2161 aagccggcgt gccgatcctg atccaggcct atccggacga gatggacaag atggcgcccg
     2221 caacacggcg cgacgccttc tgcggcaagc tctcgattat ggatgtcttc tgccagaacg
     2281 gcatcccctt cacggcgttg aagccgcaca ccgtctcgcc cgcctcgccg cgcttcgccc
     2341 agaacgtgga gtacttcgac cgcgtctgcc gcgtggtcaa ggggatgcgc aacctggtag
     2401 tcggcgctgt cggcgcgcgc accaccgcgt tcaagaccgt gcgcatcgac gaggtcgcgc
     2461 tccaacgcca ccggatcacg atggagacgt tcgacctctc cgatctcttc gcgcggatgc
     2521 gcgcagtgga cccgaatagc gacgcctacc gcgccaagga ggaccgctac ctgggctatg
     2581 ccgacttctc cagcgtaccc ggcgacaaga gggacaccct cgtccgcctc ggcgtcgccc
     2641 tcgataccct catcgaagag gcacgccttg ataccatcgc cctgcgctgc tgggaggaga
     2701 tgcagcgtca gcttggcatc tcaccgtgcg tggtcctcag cgagatgaac aaccgcggga
     2761 tcaccgcggc atgcgaggtc gatgtcggca acgccatcgc gatgcatgcc ctcggcatgg
     2821 catccggcga ggtgaccgcc tgcctcgact ggaacaacaa ctatggggat gacgacgaca
     2881 agtgcatcct cttccactgc gggcccgtgc cgcaatcgat gatgaccgcc aagggcaagg
     2941 tggaggagca cgccatcctt gccaacgcag tcggccgtag ctgcagcatc gggtgcaatg
     3001 tcggccggat cgcgcctacg tccttcacct tcgcgagcat gaccaccgat tccggccgcc
     3061 tgaagttcta tgtgggcgag ggcgagttca cgaacgaccc gatcccaccg gagttcttcg
     3121 ggtgcgccgg cgtcgcccgc gtgccggcgt tgcaagacgt gctgctccac gtcgggcgat
     3181 gcgggcatcg ccaccacgtc agcgtaacgc ccggccaggt ggccgatccc gtctgtgaag
     3241 cgctgaccaa ctacctcggc tacgacgtcg cccgtccgca accgtgacag aatggagggg
     3301 cacccggcgg tcgcgcaagc gagcgcggcg agtgcccctc ccccctctca cccaaggctg
     3361 agagactcgc ccgcgaaacg ctcccaacac agcgcgcgag ggggaaggaa agccctttac
     3421 ggcgctgaat tagtggtcaa taagccagct cggtgagcgc gtgacagcgc ggccacggaa
     3481 ggtgcgccgg ctgcttcatc gcggttacgc aagctgctcg ctccgcctgc cactccgcct
     3541 cgcagtgcgg aggcagagac gcgacacggt ttggaggaga ctatgcagag caccagacgc
     3601 ggtttcacgt tgatcgagct actcgtcgtc attgccatta tcgccatcct cgcggcgatc
     3661 ctctttccgg tgttcgcgcg cgcgagggaa aatgcgcgca agtccacctg cacgagcaac
     3721 ggacgccagc tgggtacggc gctcatgcaa tactgccagg attatgacga gacgctgcct
     3781 tacggccagc acgaggcgcc ggactcgcgc tttagtttcc aggttctcct gccttacatg
     3841 aagaacgagg agatcttcgc gtgcccctcg gataagaacg ttgtcacgtc gtttgttgcc
     3901 gacggctacg gctcggcacg cacaatcccc cacgggtggg gcgtggacca gtatcactat
     3961 ccttaccgca cgatctacag ccagccggtc actctcgcca atatttcgcg tgtttccgag
     4021 tcggccgtct tcttcgagaa gaccaacgcg gtgaccacca tgccgtacgt ctactgccct
     4081 atccacaacg cgccgctttc cacccaggtg gctggccggc acaacgacgg cctgatcgtc
     4141 gtcttctatg acgggcatgc caagtggatg aagagggaag aggtgatcgg cggtccgaac
     4201 gtcgcccggc tctggctaca cgacaactcc tgagaagcgt gcctcgcgca ggttggcgcg
     4261 gagtggcgct cggttccccg ccgcgggggg gaatccggcg ggccggcgca ggtggccacc
     4321 gcggatcacg cggtcggccg cgaagtgttg cctcaaacgg cagtcaccgg aacaacccgc
     4381 cgcagccctg tagaatcacg agaggaaagc cctcacccgc gctgaatcaa tgggcaagga
     4441 accagcccgg cgaagcgcgt agtagagcgg ccacagaagg agctccggct acctcgccac
     4501 ggtttcgcag gctactcact ccgccagcca ctccgccccg tagagcggag gtctggaaga
     4561 gctgcagaat ggttgggagg agatcatgca gagcaccaaa cgcggtttca cgttgatcga
     4621 gctactcgtc gtcattgcca ttatcgccat cctcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnncgcgcg cgagggaaaa tgcgcgcaag tccacctgca cgagcaacgg
     4801 acgccagctc ggcacggcgc ttatgcagta ctgtcaggac tatgacgaga ctctcccgta
     4861 cggccaccat gaggcaccgg actcacgcta tagcttccag tgcctcctgc cttacatgaa
     4921 gaacgagagt atcttcgcgt gtccatcgga taagaacgtc gtctcgacgt ttgttgccga
     4981 cggctacggc ccggcccgca ccatccctca tgcctggggc gtgtgccagc atcactaccc
     5041 ctaccgtgtc atctaccaac agccgatgac actcggccag atctctcgcc catccgaatc
     5101 gggcgtcttc ttcgagaaga ccaacgtggt gaccaccatg ccgtacgtct actgccctat
     5161 ccacaatccg gcgaacgtca cccaggtcgc cgaccggcac atggacggcc tgatcatcgt
     5221 tttcttcgac gggcatgcca agtggatgaa gaaggacgaa gtgatcggcg gtccgaacgt
     5281 cgcccggctc tggctgcacg acaactcctg agaatcgcgc cccggttggg gcatgtacct
     5341 tgaaacccgc ccggtttccc tgccgcggga accgggcggg cccacgcagg tggtccccgc
     5401 gtcacgcggg gggcaggagg gggagaatct ctgcttcgag acctgtttcg cggatgtaga
     5461 ggcgtgcgag ggtgacgggg aggtggaagc ggcgtgggcc ggcactcccc gg
//
LOCUS       DAOSCV010000177         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9383_length_5482_cov_2.842086, whole genome shotgun sequence.
ACCESSION   DAOSCV010000177 DAOSCV010000000
VERSION     DAOSCV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9383_length_5482_cov_2.842086"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..1097
                     /gene="dapB"
                     /locus_tag="PLY56_05785"
     CDS             294..1097
                     /gene="dapB"
                     /locus_tag="PLY56_05785"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ21024.1"
                     /translation="MQPIRVLVRGAHGRMGREVVKAVMAEPDLRLVGAIDRVGVGEDA
                     GRVAGTREAGVAIRPPEELEAVLEATDPEVAVDFTKGPEALELFRAAVPRNTSPVVGT
                     TGMADEALAEARALCEQHSVGALIAPNFAIGAVLMMKFAQEAAKYLGDVEIIELHHER
                     KMDAPSGTAKRTAQLIAEARTAQRAERPATLDEAPGARGTEQHGVPIHSVRLPGLVAH
                     QEVLFGGVGQMLTIRHDSIDRTSFMPGVVLAIRRIRGHKGLLVGLENLL"
     gene            1308..4286
                     /locus_tag="PLY56_05790"
     CDS             1308..4286
                     /locus_tag="PLY56_05790"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ21025.1"
                     /translation="MRFLLLIAALAGMTPALVPAAAAPVPDVLVLDDFEGEIRWQGVT
                     PTEEIARGGKRAGRWHNVKATPRIALENAPKDWSRYDRICFWLHSRKANKQALTLLLQ
                     SDNPKDKEGWDYYYYHFEVDWEGWRFFSLRFGHEIQASRRPRGWDQIDGVSLSATGWG
                     HTPLPDTELTLDDVVLVRDPVAVTPLPAEAREADGSLIVETTVQVTNRSSTARAFSLE
                     YDRSGLRDFRLDAPDKTPVIEPDGQARVAVRMTLPPERRAGAKPLARESVMMKVHAPG
                     ASEGAPAPPPVQVEVAANVPLPHRTRPRLFATAADLAAAKARAERYPWARTIYDQILD
                     AAVRALEAPLDVPDKGGQWPHHYACPKHGRTLQNVSPTEHRCPVDGETFSGWPYDDVI
                     ITRRHGRNSSDVVNLGLAYQWTGDRRYADKAAEIFRQYAAKYPTYAIHDTRGHESRSG
                     ARMYAQTLDEAVSFISFAWGYDLVADSGALSPEEQKAVEGRFFREAARVIQRHDAKIS
                     NWQSWHNAGVGAIGFALNDAALAAWALHGPSGLMMQLSESVLEDGWWYEGAPAYHFYA
                     LSALVYLADAARLAGIDVWRDPRMKRLFDAPLLYTYPDLRFPAVNDSDVFSLRGQAGL
                     YEIAYARFRDDRYLPILAGERKTRQALLWGVETLPTASTAAALPSHDFSALGTVVLRA
                     GEGADATYVHLDYGPHGGGHGHPDKLNLILFALGQEVSPDPGRLAYSVPAHRSWYRQT
                     VAHNTVVVDAASQAPTEGSLPFLAPGGALQVATGLSTGAYPEVTLQRTVALVAVPRRA
                     APPYLVDFYTAIAKAERTFDWVYHNRGELRGGEATPAKPLGEGEGYQHLEKLRRAADP
                     ATPVDWVLSEGGIRLWSAAPATERYFAEGMGQPPSEAIPLVLDRKRGTTAVFASVIEP
                     YRGQPAIRGVRALPVTVDGKPAAPETALALEIDRGEEKDVILLAPGIQGEKSASGVRT
                     AKQAALVVLRGTQELLREEK"
     gene            4568..4939
                     /locus_tag="PLY56_05795"
     CDS             4568..4939
                     /locus_tag="PLY56_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21026.1"
                     /translation="MQRAFSERKGTARRLMALVAICALLLAGALASYHQHGGPLPPAG
                     PSYHVGAPGAHPDESDLWDSMDGCLACLWTRAAVGCQVAAGPTVIPSQAAERVVSPDE
                     SGYRHIATLSRHSRAPPSSSL"
BASE COUNT          877 a         1901 c         1846 g          858 t
ORIGIN      
        1 cctatgcgtt cggtggcgat ttctggcggc ggatcgatga ggtcgtggag cacgtggaga
       61 ggatcaccat cgaggatgtg aaccgcgtcg cgcgcgcgat ccttcccaag gagacgactt
      121 cggtcgtcat cctgggcccg aaggcgcgct cccggcgctg accacagttg aacgcagcgg
      181 agcaggaatg cccggtagag cggacggctt tccgagaggc aggcgctcgc tcggagccat
      241 accgcttgca acttccacgc ttgcctgctc ccatcctgga ggtacagtag cagatgcaac
      301 cgatacgagt tctggtccgc ggcgcgcatg ggcgcatggg ccgcgaggtc gtcaaggcgg
      361 tcatggcaga gcctgacctg cggctggtgg gtgcaatcga ccgcgttggc gtcggcgagg
      421 acgccgggcg cgtagccggt acccgagagg ctggcgtcgc catccggccc ccagaggagc
      481 tggaagctgt cctggaggcg acggatcccg aagtcgccgt tgacttcacc aagggtccag
      541 aggcgctgga gttgttccgt gccgccgtcc cgcgcaacac ctcaccggtg gtgggcacga
      601 cgggaatggc cgacgaggcg ctggctgaag cgcgcgcttt gtgtgagcaa cacagcgtgg
      661 gcgccttgat cgcgccaaac ttcgccatcg gcgcggtgct gatgatgaag ttcgcccagg
      721 aggccgcgaa gtaccttggc gacgtggaga tcatcgagct gcaccacgag cggaagatgg
      781 atgccccctc cggcacggca aagcgcacgg cacagctgat cgccgaggcg cgcaccgccc
      841 agcgcgcgga acgcccggcg accctggatg aagcccccgg cgcccgaggc accgagcagc
      901 atggggttcc gatccactcc gtgcgccttc ccgggttggt cgcccatcag gaggtcctct
      961 ttggcggcgt tggccagatg ctgactatcc gccatgactc catcgaccgt acctccttca
     1021 tgccgggggt cgtcctcgcc atccggcgca tccgtgggca caaggggctt ctggtcggcc
     1081 tggagaatct tctttgagga gcccgaagcg cgacgccggc ggcacgaggc gcgagcgccc
     1141 cgccacgcat catcccagat cgtgaggggg caggccttgc gcccatcccg gcgccggcgc
     1201 gcgaatctcc tgcatcctga acggcgagcc tgacccaggc gcgagagccg ggcaggccac
     1261 cgacgaggcg cggcgcgcct ctctcgtgat ctggaaaggc gttttcgatg cgcttcttac
     1321 tcctgatcgc agctctggcc gggatgacgc cggccctcgt gccagcggcc gccgcgcccg
     1381 ttcccgatgt gctcgttctc gatgacttcg agggcgagat ccgctggcag ggagtgacgc
     1441 ccacggaaga gatagcgcgc ggcggcaagc gcgccggccg gtggcacaat gtgaaggcca
     1501 ctccccgcat cgccctggaa aacgcgccga aggattggtc acgctacgac cggatctgct
     1561 tctggctgca ctcgcgcaag gcgaacaagc aagccctcac cctcctgctc cagtcggaca
     1621 acccgaagga taaggagggg tgggattact actactacca cttcgaggtg gattgggagg
     1681 gctggcggtt cttctcgctc cgcttcggcc acgagatcca ggcctcgcgc aggccccgcg
     1741 ggtgggacca aatcgatgga gtgagcctca gcgccaccgg ctggggccac acgcctctcc
     1801 ccgatacgga gctcaccctg gatgacgtgg tcctcgtgcg tgatcccgtc gccgtcactc
     1861 ccctgcccgc cgaggcccgc gaggcagatg ggagcctgat cgtcgagacc acggtgcagg
     1921 tgacgaaccg ctcgtctacc gctcgagcct tctccctgga gtacgaccgc tcgggcctgc
     1981 gcgacttcag gctggacgcg ccggacaaga cgcccgtgat cgagccggac ggccaggccc
     2041 gcgttgcggt ccggatgacc ctgcccccag agcgtcgcgc gggggcgaaa cccctcgcga
     2101 gggagagcgt catgatgaag gttcacgcgc caggcgcttc cgagggcgcg cccgcgccgc
     2161 cgcccgtgca ggtggaggtc gcggcgaacg tgcccctgcc ccaccgaacg cgcccccgcc
     2221 tcttcgccac ggccgccgat ctcgcggcgg ccaaagcacg cgcagagcgc tatccttggg
     2281 ccaggacgat ctatgaccag atcctcgacg ccgccgtccg ggcgctcgaa gcccccctcg
     2341 acgtgcccga taagggcggg cagtggcccc accactacgc ctgtccaaag cacgggcgaa
     2401 ccctgcagaa cgtttcgccc actgagcacc ggtgcccggt ggatggcgaa acgttctccg
     2461 gctggcccta cgacgacgtg atcatcaccc gccggcacgg gcgaaactcc agcgacgtgg
     2521 tgaacctggg cctcgcctac cagtggacgg gtgaccggcg ctatgccgac aaggcggccg
     2581 agatcttccg gcagtacgcg gcgaagtatc ccacctacgc gatccacgac acgcgcgggc
     2641 acgagagccg cagcggcgcg cgcatgtatg cccagacgct cgatgaagcg gtgagcttca
     2701 tctcctttgc gtgggggtat gacctggtgg ccgatagcgg cgcgctctcg ccggaggaac
     2761 agaaggcggt cgagggccga ttcttccgcg aagcggcgcg agtgatccaa cgccacgacg
     2821 cgaagatcag caactggcag tcgtggcaca acgccggcgt gggcgcgatt ggcttcgcgc
     2881 tgaacgacgc cgccctagct gcctgggcgc tgcatggccc cagcgggctg atgatgcagc
     2941 tttccgaaag cgtactcgaa gacggctggt ggtatgaggg cgcgcccgct taccacttct
     3001 acgcgctgtc ggcgctcgtg tacctggcag atgctgcgcg tctcgcgggg atcgacgtgt
     3061 ggcgcgaccc gcggatgaag cggctgtttg atgccccctt gctctacacc tacccggatc
     3121 tgcgcttccc ggccgtcaac gatagcgatg tcttctcgct ccgaggccag gccgggctct
     3181 acgagatcgc ctacgcgcgc ttccgggatg accggtatct ccccatcctg gccggcgagc
     3241 ggaagacgcg ccaggccctc ctgtggggcg tggagacact ccccacggcc agcaccgcgg
     3301 ctgcgctccc cagtcacgac ttctccgcgc tcggcactgt cgtcctccgc gccggcgaag
     3361 gcgcggacgc cacctatgtg cacctggatt acggtcctca tggcggaggg catggtcatc
     3421 cagacaagct gaacctcatc ctgttcgccc tcggccagga ggtctcgccc gatccggggc
     3481 gtctcgccta cagcgttccc gcgcaccgga gctggtatcg ccagaccgtg gcacacaaca
     3541 cggtggtggt ggatgccgcc agccaggcgc cgacggaggg ttcgctcccc ttcctcgccc
     3601 caggcggcgc tctccaggtg gctaccgggc tctcgacggg cgcctatcct gaggtcacgt
     3661 tgcagcgcac ggtcgcgctg gtcgccgtgc ccaggcgcgc ggcgccgccc tatctggtgg
     3721 acttctacac cgccatcgcg aaggcggagc gcacgttcga ttgggtgtat cacaaccgcg
     3781 gcgagctgcg tggtggcgag gccactcccg cgaagccgtt gggcgaggga gagggctacc
     3841 agcacctgga gaagctccgc cgcgcggccg atcccgccac gccggtagac tgggtgctgt
     3901 ccgagggagg gatccgtctc tggagcgcgg cgccagcgac ggagcgctac ttcgccgagg
     3961 ggatgggaca accaccctcc gaggcgatcc cgctggttct cgaccggaaa cgcggcacca
     4021 cggcggtgtt tgcatccgtg atcgagccgt atcgcggcca gccggcgatc cggggcgttc
     4081 gcgcactccc ggtgaccgtt gatggcaagc cggccgctcc cgagaccgcc ctcgccctgg
     4141 agatcgaccg cggcgaggag aaggacgtga tcctgctggc acccggcatt cagggcgaga
     4201 aatcagcgtc cggggttcgt acggcaaagc aggcggcgct cgtcgtcctg cgcggcacgc
     4261 aggagctcct gcgcgaggaa aagtagcgtc ggctgatctt tcggccgggg aaaccctttg
     4321 gtgttcgccc cttcacgtcg cccgcggttc ggtggcggtg gcctgggagg gggagcccga
     4381 aggggctttg cagggaagca gcgcggagct tcgagctccg cgcgcgggca gccctctcca
     4441 gccgtgttcg tcgccttcat ccgcatctcg aaactctcgc gcctccgcgc ctccgcccgc
     4501 ggcttgacag gtgaccgcaa ggcacctata atgacgccag atgcacgcgg gtattgggag
     4561 gtgcacaatg cagagagcgt tcagcgaaag gaagggcact gcccggcgcc tgatggcgct
     4621 ggtggccatc tgtgccctcc tgctcgcggg cgcgctcgcc agctatcacc agcatggagg
     4681 ccctctacca cccgcagggc cttcctacca tgtgggcgcg cccggtgcgc atccggacga
     4741 atcggatctc tgggattcga tggatggatg ccttgcctgc ctctggacgc gtgcggcggt
     4801 tggctgccag gtggcagccg gccccaccgt catcccctcg caggcagcgg aacgggtagt
     4861 atcccccgat gagagcggct accgccacat cgccaccctg agccgccact cccgcgcccc
     4921 tccttcctct tctctctaaa cgcgtggcag ggccgcagct cgtcgcgccc gtttcgccgc
     4981 gcttcactta acgtcatttc agcataccgc cgggttcctc gtcccgccgc gacccgctat
     5041 caggggccga cacgcgcggg cttcccgacg tgggtgatcc cgtcactcgg ccccggacgc
     5101 ctggtacagg gcgttccccc ggccgggcgg catcgcgccg ctggcgccgg acggccggga
     5161 gacgcgcggc catcggggcg gtggagagaa cccgacacgc tatgctggcg gtttgtcact
     5221 cgcgcactgg caagaacccc atgacgctgc gcctggtctg gccggctgtc gtatggctcg
     5281 ccggcgcgac gcgacagtga gaagaggagt tagcatgtct cacgcacggt tgatcgcatc
     5341 cctggcgctc gccgcaatcc tgatggcagc atcctggcag cttgctcagg ccgagaccgc
     5401 accaccaccc ccgcctccac cggccgaggc agaggcggaa acggaagcca tagagggaac
     5461 cacggccccg gctcaggccg gc
//
LOCUS       DAOSCV010000178         5473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9396_length_5473_cov_3.169251, whole genome shotgun sequence.
ACCESSION   DAOSCV010000178 DAOSCV010000000
VERSION     DAOSCV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9396_length_5473_cov_3.169251"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLY56_05800"
     CDS             complement(<1..586)
                     /locus_tag="PLY56_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HOJ21027.1"
                     /translation="MDLMGTLKGSLLENFYPAGWDLAKIDACCSNPPETITERQPWWN
                     PRFTPIPCATLAEFDTYMGHEIAMQIRKAREAGRKLALILPVGPMGMYRWAVYFLKEW
                     GVKADHVYGFNMDEWSDEEGNTLPPDNPGAFRFAMEDAFYGPLGELTVPENQRHFATK
                     DMLPTYAERIAALKKEGAELNVIFGIGRVFHIAFW"
     gene            complement(767..3349)
                     /locus_tag="PLY56_05805"
     CDS             complement(767..3349)
                     /locus_tag="PLY56_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21028.1"
                     /translation="MAEEKHAPASLDECSDSRALAEFVAAGQARLRDAFRASPSGRAH
                     SLALTELFDAVIRRLFLLASREAEADVPPTGIEIAVLATGGYGRRELCPRSDIDVAFI
                     AAGEEDPYLAAVVRRMFFLIMDVFTVHSDLKVGYAFRVLDDCPELDPQTRTALLDGRL
                     IAGSEALARRFHAELLRTMRPAAFLYDRLAERERLRRKYGGSVHCTEPDLKECAGGLR
                     DLHFMGWAARAQSSQPLDDPWAYLVAIGDIEPGERARAAEAHEFLLRVRHALHYARER
                     RFDRLSRERQEAVAAALGYADGRALMTDYFAHAEWIHAGAVRVGERLRRRMLPLTDGL
                     VVKEGEIDTADPSATWPDLASIVALFRHSQVYGLPLCADLGERILAVASDLPPEPSGG
                     AAGRLFLEMLRDATGVEAVLRQMTRWGVLQRLVPEFGEALRLVPQALVHEYTIGEHSL
                     RVVGFLEQARASAAPERAYAQAFSELQRLDLLFLAALLHDIGKRTEGARHAEVGATEA
                     HAVALRLGLDPEAAEMVAFLVRHHLLMSDTARLRDLTETQVIRDFTATVATAEQLNSL
                     FLLTYADLRATGGGVWTPVQERFLEDLYYRAHHAITAPEPEEDLMVRVQRRRRRMQRE
                     LTLENLPPEAVQEHCNAMPPSYLLNTPAEVIAQHIRLVERVKVEGPVVEFLDSPGRDF
                     TTLTLCTFDDPQPGLLSKIAAALYAHSVNVHAAQVFTREGQPGIAIDTLWVDRDGRRL
                     RSFERAEIERDLKGVLSGQITVESLLQRRGKLPQADLSAADVRVHNDVSEARTVIEVR
                     ARDCSGLLYRLTHRLSTLGWDIHSARINTIGGDVRDAFYVTNHQGEKLVDDPAVIRAR
                     LLEP"
     gene            complement(3433..3795)
                     /locus_tag="PLY56_05810"
     CDS             complement(3433..3795)
                     /locus_tag="PLY56_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ21029.1"
                     /translation="MEGNGQVVAREGPADLTVTIVEGTPPVVVLAGEVDLATAPEFEK
                     ALLSLLSQGRDSLAVDLGRVTYLDSTGINVLMKAVKLCSERGGKLAIVAASERAMRVM
                     RLLNLDRVMPIRQSAPMP"
     gene            4104..4589
                     /locus_tag="PLY56_05815"
     CDS             4104..4589
                     /locus_tag="PLY56_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HOJ21030.1"
                     /translation="MERESTQSRWAINPRRALALLRITLGLLLLGAFLENYQKGLYTA
                     ETYAGYIESLITRGATPDFWKGALQTLAEHAATVAPLQAVLELSLGGLLFLGLLTRLA
                     AGTTFLLLSARWLSEWGIASVGEMAFPMIVALLLAFSNAGHWAGLDGVLSRRRPTLTF
                     G"
     gene            5006..>5473
                     /locus_tag="PLY56_05820"
     CDS             5006..>5473
                     /locus_tag="PLY56_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ21031.1"
                     /translation="MDKELLQKWFYQMKLIRVFEERCAELYAYGKIGGFLHLYIGEEA
                     IAVGALAAVTDEDHLLTHYRDHGWALARGTDPKPLMAELLGKSTGVSKGKGGSMHLCD
                     PERNFYGGYAIVGGHLPLATGIGFAMRYQGSDRVVVAVFGDGATNIGEFHEAMN"
BASE COUNT          992 a         1826 c         1766 g          889 t
ORIGIN      
        1 cccagaaggc gatatggaac acgcgcccga tgccgaagat cacgttcagc tccgcgcctt
       61 ccttcttcag cgcggcgata cgctcggcat aggtaggaag catatcctta gttgcgaagt
      121 ggcgctggtt ctcgggaacg gtcaattcgc caagagggcc atagaaggca tcttccatgg
      181 caaagcgaaa agcgcccggg ttatccggcg gcagcgtgtt gccttcctca tcggaccact
      241 cgtccatgtt gaagccatag acgtggtctg ccttcactcc ccactccttg aggaagtaga
      301 ccgcccagcg gtacattccc atcggcccga cgggcagaat gagggcgagc ttgcggccgg
      361 cctcccgcgc cttccgaatc tgcatggcga tctcgtggcc catataggtg tcaaactccg
      421 cgagcgtggc gcaagggatc ggcgtgaagc ggggattcca ccagggctgc cgctcggtga
      481 tcgtctccgg gggattgctg cagcacgcat caatcttggc caggtcccag ccggcgggat
      541 agaagttctc cagcagggag cctttcaagg ttcccatcag atcgatcgag cctgtcggtt
      601 tactcatcgg cgtacctcca aacggtgtga ttcccagcgg gtggtcgcat ggactatcat
      661 acccttccat gccagaagcc agagtcctgc cagcgggagg agggcaagcg ggtgcttctc
      721 ccccgctcca gggggcagcg ccgcagtctc tcaccgggga gcgaccctac ggctccagca
      781 gacgggcacg gatcaccgca ggatcgtcga caagcttttc gccctgatgg ttggtgacgt
      841 agaaggcatc gcggacgtcg ccaccgatgg tattgatgcg cgccgagtgg atatcccagc
      901 ccagcgtgga gaggcggtgc gtcagccggt agaggagccc ggagcagtct cgagcacgca
      961 cctcgatcac ggtgcgcgcc tcggaaacat cgttgtggac gcgcacatcc gccgccgaaa
     1021 gatctgcttg ggggagcttt ccacgccgct ggaggaggct ctccacggta atctgccccg
     1081 acagcacccc cttgagatcg cgctcgatct cggcgcgttc gaaggatcga aggcgccggc
     1141 catcccggtc cacccagagc gtatcgatgg cgatccctgg ctggccctcg cgcgtgaaga
     1201 cctgcgcggc gtgcacgttc acgctatgcg cgtagagcgc cgccgcgatc ttgctcagca
     1261 ggcccggctg cggatcgtcg aaggtacaca gggtcagcgt tgtgaaatcg cgcccgggcg
     1321 aatcgaggaa ctcgacgacc ggtccctcta ccttcacgcg ctcgacaagc cggatgtgct
     1381 gcgcgatcac ctccgccggc gtgttgagca ggtaggaggg cggcatggca ttgcagtgct
     1441 cctggaccgc ctcgggaggg agattctcga gcgtcaactc gcgctgcatg cgccgccgcc
     1501 ggcgctgcac acggaccatc aggtcctcct ccggctccgg cgcggtgatg gcgtggtgcg
     1561 cgcgatagta gagatcctcc aggaagcgct cttgcacggg agtccaaacc ccaccgccgg
     1621 tggcgcgcag atccgcgtag gtgagcaaga agaggctgtt gagctgctcg gcagtcgcaa
     1681 ccgtcgccgt gaagtcgcga atcacctgtg tctcggtgag gtcgcgcaag cgcgccgtgt
     1741 cggacatcag gagatggtgg cgaaccagga aagcgaccat ttcggcggcc tcggggtcga
     1801 ggcccagacg cagcgcaacc gcgtgggcct cggtggcgcc cacttccgcg tgacgcgcgc
     1861 cctcggtgcg cttgccaatg tcgtgcagga gcgccgccag gaagagaagg tcgagacgct
     1921 gcagttcgga aaacgcctgc gcataggcgc gctcgggcgc ggcactggcg cgcgcctgct
     1981 ccaggaaacc aacgacgcga agcgagtgct cgccaatagt gtactcgtgg acgagcgcct
     2041 ggggaacgag gcggagtgcc tcgccgaact ccggcaccag gcgctgcagc acgccccagc
     2101 gtgtcatctg ccgcagaacc gcctccaccc cggtggcatc gcgtagcatc tcgagaaaga
     2161 ggcgtccggc agcgcccccg gatggctctg gaggcagatc gctcgccacc gccaggatgc
     2221 gctctcccag atcggcgcac agcggcagcc catacacctg actatggcgg aatagcgcca
     2281 cgatgctcgc caggtccggc caggtggcgc tcggatcggc tgtatcgatc tcgccctctt
     2341 tgaccaccag gccgtcggtg agcgggagca ttcgccggcg caggcgctcg ccgacgcgca
     2401 ccgccccggc gtggatccac tccgcgtgcg cgaagtagtc ggtcatcagc gcgcgcccgt
     2461 ccgcataacc aagtgccgca gccaccgcct cctgacgctc tcggctgagg cggtcgaaac
     2521 gacgctcgcg tgcatagtgc aaggcatgac gcacgcgcag gaggaactcg tgcgcctcgg
     2581 ccgcgcgcgc gcgttccccg ggctcgatat cgccgatggc aaccaggtag gcccatggat
     2641 cgtccagggg ctggctcgac tgggctcggg ccgcccaccc catgaagtgg agatcgcgca
     2701 atccgccggc gcactccttg agatccggct cggtgcagtg aaccgagccg ccatacttgc
     2761 gtcgcaggcg ctcgcgctcg gcaagccggt cgtagaggaa ggcagccggg cgcattgtgc
     2821 gcagcaactc ggcatggaag cgacgcgcca gcgcctcgct cccggcgatc agacggccat
     2881 ccagcagcgc cgttcgcgtc tgcgggtcca actcggggca atcatccagg acgcgaaacg
     2941 catagcccac cttgagatcc gagtggaccg tgaagacgtc catgatcagg aagaacatgc
     3001 gccggacgac ggcagcgagg taggggtcct cttccccagc ggcgatgaag gcgacgtcaa
     3061 tatcggaccg ggggcagagc tcgcgccggc catatccccc ggtggccagg acggcgattt
     3121 cgatcccggt tggcggaaca tcggcctccg cttcgcggct ggccaggaga aacagccgac
     3181 ggatcaccgc atcgaaaagc tcggtgagcg ccaggctgtg cgcccggccc gaaggagacg
     3241 cgcgaaaggc gtcgcgcagg cgcgcctgcc cggcggctac gaactccgcc agcgcgcgcg
     3301 aatccgaaca ctcatccagg gaggcggggg cgtgtttctc ttctgccatc tttcctcgcg
     3361 aaccaggagc cgtctgaggc caggccgaaa tgccgcggcc tgcccaaacg tatcacaccc
     3421 ctggcacgcg tgtcaaggca tcggcgcact ctgccggatc ggcatcaccc ggtccagatt
     3481 gaggagacgc atcacccgca tagcgcgctc ggatgccgcc acgatggcca gcttccctcc
     3541 ccgctcgcta cacagcttca ccgccttcat caagacgttg atcccggtgc tatccagata
     3601 ggtcactctc cccaggtcca ctgccaggga gtctcggccc tggctcagga gcgagaggag
     3661 agctttttcg aactcgggcg cggtagcgag atccacctcg cctgccaaaa ccacgaccgg
     3721 cggagtgcct tcgacaatgg tcaccgtcaa atcggccggt ccttccctcg cgaccacctg
     3781 gccattgccc tccatcacta tctccacggg cccggccacc acagggtgtc acctccatgc
     3841 tccaccccgg cagacgcgcc cgacatcgca aacccaccgc tccggcggct gcccgtccgg
     3901 cggcacgcct tcatgccaac tggcaacacc ctatattccc aaccggacgc ctccgcaaac
     3961 gccggtcgag ggcatacggt gtggctacat ccgggcccgc cgggcgcgac ccgagagcga
     4021 aagcattcgt gtttgaccca cgggcggaag gggtaggctg gaggggtaca ccaccccgcg
     4081 aaacccacga gagggggaat ctcatggaga gggaatcgac ccaatcacgg tgggcgatca
     4141 atccgcgtcg cgccctggcg ctgctgcgga tcacgttggg gctgctcctc ctcggcgcgt
     4201 ttcttgagaa ctaccagaag gggttgtaca cggcggagac ctatgccggg tacattgaga
     4261 gcctgatcac acgcggggca acgcccgatt tctggaaagg cgccctgcag accctcgcag
     4321 agcacgctgc cacagttgct cccctgcagg cggtcctcga gctgagcctc ggtggcctcc
     4381 ttttcctcgg tcttctcacc cgcctggccg ccggaacgac gttcctcctc ctcagcgcgc
     4441 ggtggctctc cgaatggggc atcgcgtcgg tcggggaaat ggcttttccc atgattgtcg
     4501 cgctgcttct cgcattcagc aacgcgggcc actgggccgg gctcgacggc gtcctgtcgc
     4561 gccgccggcc aaccctcacc ttcggctagc ctccccaacg gcggcgcccc ctcacgccac
     4621 ccggtactct cggacccttt cagggtccca attgtatcct ggccacaaga cccagggtca
     4681 ggagaccctg ggctaggatg tcggacgcct ccggcatcct ccgagtctcc ccgagcagtc
     4741 tgaccacgat cccaggtaag gcccacgatc ccaggcacgc gccgctgaac ttgacgcgcc
     4801 cgggaatgcg tggaacagta ggagtacccc gcgcgcgatg gaagagactc atgggtagca
     4861 ggcgcgcgga gtaggcacgg tttaggtacg cccgctttgc gggaagaata gggagatgga
     4921 cgcgccagcg tccgggtgcg gtttgcagcg ccttctttgc cggcgggcta acccggttgg
     4981 cttccgttcg gcgggtgagg agaacatgga caaagagctt ctccaaaagt ggttctacca
     5041 gatgaagctg atccgcgtct tcgaggaacg atgcgcggag ctgtatgcct atggcaagat
     5101 tggcggcttc ctgcacctgt acatcggtga ggaggccatc gcggtagggg ctctggccgc
     5161 ggtgaccgac gaagaccacc tgctgaccca ctaccgcgac catgggtggg ccctggcccg
     5221 cggaaccgac cccaagccgc tgatggcaga gctgctgggg aagtccaccg gtgtcagcaa
     5281 gggcaagggc ggatcgatgc acctgtgtga tccggaacgg aacttctatg ggggatacgc
     5341 cattgttggc gggcacctgc cgctggcaac aggcatcggg ttcgccatgc gctaccaggg
     5401 gagcgaccgg gtggttgtcg ccgtctttgg cgacggcgca acgaacatcg gggagttcca
     5461 cgaggcgatg aac
//
LOCUS       DAOSCV010000179         5471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9402_length_5471_cov_3.524926, whole genome shotgun sequence.
ACCESSION   DAOSCV010000179 DAOSCV010000000
VERSION     DAOSCV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9402_length_5471_cov_3.524926"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLY56_05825"
     CDS             complement(<1..258)
                     /locus_tag="PLY56_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOJ21032.1"
                     /translation="MNTFGYDTYLSPATWRYGSREMRAIFSEVNRRRHWRKVWVAIAQ
                     TKCEIGLITPEQLADIEAHANDVNVERSLEIERTLQHDLAAE"
     gene            complement(272..757)
                     /gene="purE"
                     /locus_tag="PLY56_05830"
     CDS             complement(272..757)
                     /gene="purE"
                     /locus_tag="PLY56_05830"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOJ21033.1"
                     /translation="MRVAVIMGSDSDLPVMKEAVAILKELGVETEQRILSAHRVPHRC
                     AEFAENAEKNGIEVIIAGAGGAAHLAGVVAALTPIPVIAVPIRSAALNGLDSLLSMVQ
                     MPPGVPVATVGIDAARNAGILAAQILGVKDARIRENVRAYKARMASQVEAKDRALQEQ
                     Q"
     gene            complement(811..2091)
                     /gene="purD"
                     /locus_tag="PLY56_05835"
     CDS             complement(811..2091)
                     /gene="purD"
                     /locus_tag="PLY56_05835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ21034.1"
                     /translation="MRVLVLGGGGREHALVWKLRQSPRVTDLFACPGNAGIAQMAETV
                     QLSLDDPNALAQFAIENRIDLTFAGPEAPLVAGVGDMFGARLLHLFGPGKAQARLEGS
                     KVYAKKLMRELGIPTAEFEVFADATKAIEYARAANRPLVVKADGLAAGKGVIVCDGPA
                     EAEDAIRQIMVDQAFGAAGKEIVIEERLEGEEATLMFFLDGRTSAPMLPSQDHKRRDE
                     GDAGPNTGGMGCYAPVPAVPEALVAEVQQGVVAPILEALDLRGIPYKGVLYVGLMLTR
                     EGYRVLEFNVRFGDPEAQTVLPLLESDLVDVATTVIDGGLYRLKPRWSSQKALTVVLA
                     SGGYPGSYETGKVIEGLEDAAKVPGVVVFHAGTAERDGQIVTAGGRVLNVTAVGETHA
                     EAAKRAYEAVSKIHFEGMYYRRDIGWRVISKEQA"
     gene            complement(2107..3486)
                     /gene="dnaB"
                     /locus_tag="PLY56_05840"
     CDS             complement(2107..3486)
                     /gene="dnaB"
                     /locus_tag="PLY56_05840"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ21035.1"
                     /translation="MSLQRAARVPPQSLEAEQATLGAMLLERAAIEKACELLQPEDFY
                     REAHRIIFQAAVDLSDRSEPVDLTTLAAELENRGQLEQAGGRPYLAALLDAVPTAAHV
                     EYYARIVEQKAILRRLITVGTEVVETCFGEVENIEDVVDRAESAILSVAQRRTGRAFT
                     PLKPLLDEAMDSIERLYRDKVETTGVPSGLRALDEMTNGFQKSDLIIVAARPSMGKTS
                     LCINIGVHAAVHAKVPVAIFSLEMSKEQLAMRIIQSEARVDSRRLRTGHLNEEQWRRM
                     TEGVNRVWHAPIYIDDTPGISAMEMRGKARRLRAEVGPLGLVIVDYLQLMRWHGRVES
                     RQQEISEIARSLKNLARELEVPVIALSQLSRAVERREDKRPILSDLRESGSIEAEADL
                     VMMLYRPSYYQYDREEDAEDVEQAGAERVDETQLIIAKHRNGPTGTVDLIFMPDYARF
                     GDQADAGYY"
     gene            complement(3508..4071)
                     /gene="rplI"
                     /locus_tag="PLY56_05845"
     CDS             complement(3508..4071)
                     /gene="rplI"
                     /locus_tag="PLY56_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ21036.1"
                     /translation="MRVLLLHDVPGVGKAGEIANVADGYGRNYLLPRKLAQLATAGLT
                     RNLEFQQKAIQRRAERERADAEAVARRIEAEPITIEVNTGVGGKLFGSVTSQDIANQI
                     QEKHGIEVDKRKIELAEPIKLVGTYTVPVRFMRDVVAQLVVNVVAVGGETAEATQEAA
                     EAPAEAAAEAPAEAAAEAAPEAAAQGE"
     gene            complement(4145..>5471)
                     /locus_tag="PLY56_05850"
     CDS             complement(4145..>5471)
                     /locus_tag="PLY56_05850"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOJ21037.1"
                     /translation="LISHVGRVGLILVVGAVVFSGIFYLLSGRYAQRNSYSITVVFDN
                     ALGLQYGSKVRMAGVDIGMVADVGLTPDNRARLRLNIDKKYGIPEGSRFTIATGALIG
                     EKFLEILPARSSRLMEPETIVQGPQQVQKPFQIEEVGTEVNKLLADLQKIAKSLDTIL
                     SNRDLQRGVVETVTNLARTTARTERLIAEAQEVVLRNQSQVDQTMANLAQASIELRAA
                     IAEVHGVVKRPDLQENVVETAAALRTASERLDAVVQDVRDITSDPDVSRNLRESAQNL
                     SEATAHARDVGERVSRFLGSGQPKITVNLPKPKLQSGSRFVIEAAGSGSGNTRTDVIF
                     TLPTGQNTGLRLGLYDFTEADKIIAQRVHWVDPKTAMRYGIFGGHPGVGMDYDISRKF
                     SMSADLYHPKYPRLDVRGRYFLTKDLGTVFGVDGILRQPRAIVGIQWRQ"
BASE COUNT          930 a         1917 c         1625 g          999 t
ORIGIN      
        1 ctcggcggcg aggtcgtgct gaagcgtgcg ctcgatctcc agcgagcgct cgacgttcac
       61 gtcgttcgca tgtgcctcga tatcggcgag ctgctccggc gtgatcagcc cgatctcgca
      121 cttcgtctgc gcgatggcga cccatacctt gcgccagtgc cggcggcggt ttacctccga
      181 gaagatcgcc cgcatctccc ggctgccata gcgccatgtg gcgggggaaa ggtaggtgtc
      241 atatccaaag gtgttcacgg tgatctcttc cctactgctg ttcctgaagc gcgcgatcct
      301 tcgcctccac ctgagaagcc atgcgggcct tgtaggcgcg cacgttctcc cggatccgcg
      361 cgtccttgac gccgagtatc tgcgccgcga ggatgccggc gttgcgggcc gcgtcaatgc
      421 ccaccgtggc cacaggcacg cccggaggca tctgcaccat cgaaaggagc gaatcgagcc
      481 cgttgagcgc agccgaccgg atcggcacgg cgatcaccgg gatgggcgtc agcgcggcaa
      541 cgactcccgc caggtgcgcg gcgccccccg ccccggcgat gatcacctcg atcccgttct
      601 tttcggcgtt ctcagcaaac tccgcgcagc ggtgcggcac ccgatgcgcc gagagaatcc
      661 gctgctccgt ctcgacgccc agctctttca ggatcgccac cgcttccttc atcacgggca
      721 ggtccgagtc actccccatg atcaccgcga cacgcatgca accaacccct tcatgcctca
      781 ttcatcgatg aaaccagccg cccggtaagg ctacgcctgc tccttcgaga tgacgcgcca
      841 gccgatgtcg cgccggtagt acatcccttc aaaatggatc ttcgagaccg cctcgtaggc
      901 gcgcttcgcc gcctcggcgt gcgtctcccc cactgccgtc acgttcaaca cgcgcccgcc
      961 ggccgtgaca atctggccgt cgcgctctgc cgtgccggcg tggaacacca ccactcccgg
     1021 caccttcgcc gcatcctcaa gcccctcgat caccttgccc gtctcgtagg agcccggata
     1081 gccgccggac gccaggacca ccgtcagcgc cttctgcgac gaccatcgcg gtttcaggcg
     1141 atagagcccg ccatcaatca cggtggtggc gacatccacc aggtcgctct ccaggagagg
     1201 caacaccgtc tgcgcttccg ggtcgccaaa acgcacgttg aactcaagca cccggtagcc
     1261 ctcgcgcgtg agcatcaaac cgacgtagag cacgcccttg tagggaatgc cgcgcagatc
     1321 gagcgcctcg aggatcggcg cgaccacgcc ctgctggacc tcggcgacga gcgcctccgg
     1381 caccgcgggc accggggcat agcaccccat gccgcccgta ttcggcccgg catcgccttc
     1441 atcgcgccgc ttgtggtcct gcgaggggag catcggcgcg gaggtgcgcc catccaggaa
     1501 gaacatgaga gtcgcctctt cgccctcgag acgctcttcg atgacgatct ccttcccggc
     1561 ggcgccaaag gcctggtcca ccatgatctg gcggatcgca tcctcggctt cggcggggcc
     1621 atcgcagacg atcacgccct tgcccgctgc caatccatcc gccttcacca cgagggggcg
     1681 attcgcggca cgcgcgtatt caatcgcttt cgtggcatct gcgaagacct cgaactccgc
     1741 cgtgggaatc ccgagctcgc gcatcagctt cttcgcgtag accttgctgc cctccagacg
     1801 ggcctgcgcc ttgccaggcc cgaacaggtg aagaaggcgc gcgccaaaca tgtcgcccac
     1861 gcccgcaacg agcggtgcct ctgggcctgc gaaggtgaga tcgatccggt tttcaatggc
     1921 aaactgcgcc agcgcgttcg ggtcatcgag ggagagctgg accgtctcgg ccatctgcgc
     1981 gatgccggcg ttgccggggc acgcgaagag gtcggtcacc cgcgggctct gccgcagttt
     2041 ccacaccagc gcatgctcgc gcccgccccc acccagtacc agtactctca cagtgttctc
     2101 cagttcctaa tagtagcccg catcggcctg gtcgccaaag cgcgcgtagt ccggcatgaa
     2161 gatcaggtcc acggtgccgg tggggccatt gcggtgcttg gcgatgatca gctgcgtctc
     2221 atccacgcgc tcggccccag cctgctccac atcctccgca tcctcctcgc ggtcatactg
     2281 atagtagctg ggccgataga gcatcatcac cagatcggct tccgcctcaa tcgagccact
     2341 ttctcgcagg tccgagagga tcgggcgctt gtcttcgcgg cgctccacgg cgcgcgagag
     2401 ctgcgaaaga gcgatgacgg ggacttccaa ctcgcgggcc aggttcttca gggagcgggc
     2461 aatctccgaa atctcctgct ggcggctctc gacgcggcca tgccagcgca tgagctggag
     2521 gtaatccaca atcacgaggc caagcggccc tacctcggcg cgcaggcggc gcgccttgcc
     2581 acgcatctcc atggcggaga tgccgggcgt atcgtcaata tagatcggtg catgccagac
     2641 gcggttgacc ccctccgtca tccggcgcca ctgctcctcg ttcaggtgcc ccgtacgcag
     2701 ccggcggcta tccacccgcg cctccgactg gatgatgcgc attgcaagct gctctttcga
     2761 catctccagg ctgaagatgg cgacggggac cttggcatgg acggccgcat gcacgccaat
     2821 gttgatacag aggctggtct tgcccatgct ggggcgcgcg gcaacgatga tcaggtcgga
     2881 cttctggaag ccgttggtca tctcatcgag ggcacgaagt ccggaaggca ccccggtcgt
     2941 ctccacctta tcgcgataga gacgctcgat ggagtccatc gcctcatcga gcaggggttt
     3001 gaggggtgtg aaggcacgtc cggtgcgccg ctgggctacg gaaaggatag cgctctcagc
     3061 acggtcgaca acatcctcga tgttctcgac ctcgccaaag catgtctcga ccacctcggt
     3121 gcccacggtg atcaggcgcc gcaggatggc cttttgctcg acgatgcgcg cgtaatactc
     3181 gacgtgcgcc gcagtcggaa ccgcgtccag cagggcggcc aggtagggcc ggccaccggc
     3241 ctgctccagc tggccgcggt tctcgagctc ggctgcgagc gtggtgaggt ccaccggctc
     3301 gctacgatcg gagaggtcta ccgccgcctg gaagatgata cgatgcgcct cgcggtagaa
     3361 atcctccggc tggaggagct cgcaggcttt ctcgattgcg gcgcgctcga ggagcatcgc
     3421 gcccagggtg gcctgttcag cctccagtga ttgcggaggg acgcgggcag ctctctgtaa
     3481 ggacatcttc cagggccctc aagatcgcta ttcgccctgc gccgcggcct cgggcgcggc
     3541 ttccgccgcg gcctcggcag gcgcttcggc agcagcctcg gctggcgctt ccgcagcttc
     3601 ctgcgttgcc tcggcggtct cgccaccaac cgcgacgacg ttcaccacca actgcgcgac
     3661 cacatcgcgc atgaagcgga cgggcaccgt gtaggtgcca actagcttga tcggctccgc
     3721 gagctcgatc ttccgcttgt cgacctcaat gccatgcttt tcctgaatct gattggcgat
     3781 atcctgggag gtgacggagc caaacagctt gccgcccacg cccgtattca cctcgatggt
     3841 gatcggctcg gcctcgatgc gccgcgcaac cgcttccgca tccgcgcgct cgcgctcggc
     3901 gcgccgctgg atcgccttct gctggaactc cagattgcgc gtcaggcccg cggtcgccag
     3961 ttgcgccagc ttccgcggca gcaggtagtt gcggccgtag ccatccgcca cgttggcgat
     4021 ctcgcccgcc ttgcccacac caggaacatc atgcaacagt agcactctca cccgcgtcta
     4081 tactccttcc tttgccgggc ggcacggggt gcccccacgg caaacgccaa caggcatccg
     4141 cccatcattg acgccattgg atgccaacaa tggctcgggg ttggcgcagg atcccatcca
     4201 ccccgaacac cgtcccgagg tccttcgtga ggaagtagcg accccgcaca tcgagccggg
     4261 gatactttgg atggtaaaga tccgccgaca tgctgaattt cctgctgatg tcgtaatcca
     4321 tgccgacccc gggatggccc ccgaagatcc catagcgcat cgctgtcttg gggtccaccc
     4381 agtgaacgcg ctgcgcgatg attttgtctg cctccgtaaa atcgtagaga ccgaggcgca
     4441 gaccggtgtt ctgccccgtc gggagcgtga agatcacatc ggtgcgcgta ttgcccgatc
     4501 ccgagcccgc cgcctcgatg acaaagcgcg agccgctctg cagcttcggt ttcggcaagt
     4561 tcaccgtgat cttcggctgt ccagagccaa ggaagcggct cacgcgctcg cccacatcac
     4621 gcgcgtgcgc cgtcgcctcg ctcaggttct gcgccgattc gcgcagattc cgcgacacgt
     4681 ccggatcgct tgtaatgtcg cgtacatcct ggaccacggc atccaggcgc tccgaggccg
     4741 tgcgcaaggc tgccgcggtc tccaccacgt tctcctgtag atccggccgc ttcaccacgc
     4801 cgtggacctc cgcgatggcg gcgcgcagct cgatggacgc ctgcgccagg ttcgccatcg
     4861 tctggtccac ctgcgactga ttgcgcagca ccacctcctg cgcctccgcg atcagccgct
     4921 cggtgcgcgc cgtggtgcgc gccagattgg tcaccgtctc caccacgccg cgctgcaagt
     4981 cgcggttgct caggatggtg tccaggctct tggcgatctt ctgcaaatcg gcgagaagct
     5041 tgttgacctc ggtgccaact tcctcgatct gaaatggctt ctgaacctgt tgcgggccct
     5101 ggacaatcgt ctccggctcc atcaaccggg aggaacgggc gggcagaatc tccaggaact
     5161 tctcgccgat cagggccccg gtggcgatag taaagcgtga cccctcgggt attccgtact
     5221 tcttgtcaat attcaggcgg agcctggcgc ggttatccgg cgtcagcccc acatccgcga
     5281 ccatcccaat gtccactccg gccatgcgca ccttggaccc atactgcaat ccgagggcat
     5341 tgtcgaacac caccgtgatc gagtaggaat tcctctgcgc gtatcggcct gagagcaggt
     5401 agaagatgcc gctaaagaca acggcaccca ccactagaat caggcccact ctgccgacat
     5461 ggctaatcag g
//
LOCUS       DAOSCV010000180         5456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9444_length_5456_cov_3.482503, whole genome shotgun sequence.
ACCESSION   DAOSCV010000180 DAOSCV010000000
VERSION     DAOSCV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9444_length_5456_cov_3.482503"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PLY56_05855"
     CDS             complement(<1..1089)
                     /locus_tag="PLY56_05855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ21038.1"
                     /translation="MRIGHVTFGYRPTLGGAETYLVELIRVLAEAGHTQRVYQRSTGD
                     AGPDILRVPTCRRLSPGMQFWSLAATLPLRWPWLAREDLLIVHYPLYALPVLWHPRMI
                     GLSHGVTWDDRPGSRAARLKKRLARIAFARSRAYVANDTFFLREMGLNAPPGQGAFQQ
                     VAPGRWFIPNCVDTEWFVPTPGQDELRALNAVLVPRNLYRNRGIHLAIAAFPRVLEAY
                     PETHLVIVGGEGDPGYGEEVRRQVANSGVQHRVRFLGSVPWREMRAVYSAARVTVIPS
                     ICGEGTSLSALESMACGTPVVATAVGGLPDLPAWLAEPNPDALSEAILCVLARRDEVA
                     RAQQAEVRESYHLGRWAAAWRQVVKEVIE"
     gene            complement(1324..5325)
                     /locus_tag="PLY56_05860"
     CDS             complement(1324..5325)
                     /locus_tag="PLY56_05860"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ21039.1"
                     /translation="MPLRMERLRGIAAVREGATPLAWQPAEGGVLVLLPGTTSTGMAR
                     RIVVEAGKHADPPRSDLQVTETANTITIANLYYRVEHPKRGNGGFPTSIRFNQSGVVE
                     KGFVFEDRLFEKTRGSQAWRSDAEATARVLEAGPVQVVIETRAKAAKEPGNPRAVYRY
                     HYRASSPVVEVTARIERDDDFTWSELHFLQISRKDSSFGRWAGGEPLQTGVFTDARKG
                     YALSRWAMMANDEDAIGLSLNGTVSLYDGANSYYNYIQQTMGPFRAPAADLRARVYLG
                     PARAPEEIRARLAEESQLQVHEDVAPGGHDRSVPSPQPVSIASGSLRLGFARPEEGLG
                     ATSLFSHRTGLEFLTPSEKPLVWRLVLRGRDLPDLRIDNTAAARCSAAETPSKDGKRL
                     ELTWEDIDTGEEKGALRVRVTVDLPKASPGMSLWRIDVDNSSERYGLWEVHFPLFARL
                     SEKGMPDVAVPRGNWGYLYRSASAQIFGSYPSADWPMQFLLVNEGEHGLYLAAHDPGA
                     SPKHFAFTPGGEFRLTTPVEGMGVPGSDFRAPFPVAIGVYQGDWWQGAKRYREWALKQ
                     PWTRRGPVATRKDMPEAMKRLGLWMLASGTREQVVPRMLEAKRLFGVPMGVHWYNWHQ
                     IPFDVHYPDYFPTKPGFAEGVRELVKEGIIAMPYINGRLWDTANDNFAVGRAAACKQP
                     DGTSTYIEEYGSGAKLAPMCPATKLWQEKVNEIVGRLIRECGVNAIYLDQIGAARPQL
                     CFDPTHGHPLGGGRHWVDGYREMLDRVRAQTAGRLGITTENNAEPYMDNVDAFLVWNP
                     RRDAEIPMMTAVYSGYTLYFSSPARTMEPLAFRMVQGRDFLWGCQPGWMGFELLDPAN
                     RENAEYLREVARYRAVAAEFVVTGELIGELRPVAPLASLEAEWSTGRGKAPAALPATL
                     GTVWRARDGHVGLLIANLSDSFQRYAFRFEPGAWGLSGSDQWVVRRLTPGAGKGHAAS
                     PIALVSANDESLTQALAPREIRVLDLAPVRPGQASAIRARIDRDGQAAPTPEELPQAP
                     RTDVRFVTPPRAGETTELNVCVWGGAKPVTDVRLLLHLPASWSVEPGRALVIDRLEPG
                     EVLSFPVRCQVPEGAAGSVTVRADLVTPQPERRVPVAPVRPAATARRFAQPPTLDGDL
                     SEWQGVEPIAVSAQAQSKVNGWKGTTDLAGKVRVAWDAKNLYVAAEVTDDHFDQSYRE
                     KEIWRGDCIQIAFRPSGPAPTPGYDGVHEFGLALTPGGPLIWKWLPDEHTVTTGTLVI
                     KRVPGGLVYEAAIPWLEVGQATPVPGGCLGFSFTINDADGDGFRGWMEWTPGICGDGK
                     DASPFGRLSLLSE"
BASE COUNT          936 a         1883 c         1766 g          871 t
ORIGIN      
        1 ctctatgacc tccttcacca cctgccgcca cgcggcagcc caacgcccta ggtggtagct
       61 ctcgcgcacc tcggcctgct gagcgcgcgc aacctcgtct cgccgggcaa gcacgcacag
      121 gatcgcctct gacagcgcat cggggttcgg ctccgccagc catgccggga gatccggcag
      181 gccaccgacg gcggttgcca cgacgggcgt gccgcacgcc atgctctcca acgcggaaag
      241 cgaggtgccc tcgccacaga tggaggggat cacagtcacg cgcgctgccg aatagacggc
      301 tcgcatctca cgccagggca cgcttccgag gaaacgcacg cggtgctgca ctccgctatt
      361 cgcgacctgg cggcgcacct cctccccgta gccagggtcg ccctcccccc ccacgatcac
      421 caggtgggtc tccggatagg cctccagaac tcgtgggaaa gcagcgatgg cgaggtggat
      481 cccgcggttt cggtagaggt tgcgcggcac caacacggcg ttgagggcac ggagctcatc
      541 ctgccccggc gtcggcacga accactccgt gtcgacgcag ttggggatga accaccgccc
      601 cggggcaacc tgctggaacg ccccctggcc cggcggggca ttcaggccca tctcgcgcag
      661 gaagaaggta tcgttggcca cataggcacg gctgcgcgcg aaggcaatcc gggccagccg
      721 tttcttcaag cgagccgcgc gggatccggg ccggtcgtcc caggtcaccc catgtgatag
      781 cccgatcatc cgcgggtgcc atagcacggg cagcgcgtag agcgggtagt gaacgatgag
      841 gagatcctcg cgagcgagcc agggccagcg caggggcagg gtggccgcca acgaccagaa
      901 ctgcattccg ggcgagagcc gccggcaggt tggcacccgc agaatatccg ggcccgcgtc
      961 tcccgtgctg cgctggtaaa cccgttgggt atgccccgcc tcggccagga cgcgaatcag
     1021 ctcaaccagg taggtctccg cgccccccag tgttggccgg tatccaaagg tgacgtgccc
     1081 gattcgcatg gtggtttcgt gtggccggcc gcacccgcgg cgcgccggct tcattctacc
     1141 acgggagcgc cgtcacacgc aaacacgaga ccagacgcgg ggagagcagg cgggcgcgga
     1201 ggcgaaccgc cgcgcgatac aggatgggcg gtgttcaccc gcgcgatgca cgcagcacgg
     1261 gccccggaga aggcaagcca ggaggcgcgg aaggcgcctc ctggatcgtg cggggttact
     1321 cccctactcc gataacaggc tcagacggcc gaagggcgag gcatccttgc cgtcgccgca
     1381 gatgccgggg gtccactcca tccaaccgcg gaagccgtca ccatcggcgt cgttgatcgt
     1441 gaaggagaag ccgaggcagc cgccgggcac aggcgtcgcc tggcccacct ccaaccaggg
     1501 gatcgcggct tcgtagacga ggccgcccgg cacgcgcttg atcaccagtg tccctgtggt
     1561 gaccgtgtgt tcgtctggca gccacttcca gatgagcggg ccgccgggcg tcaaagccag
     1621 gccaaactca tgcacgccgt catatcccgg agtgggtgcc ggaccggaag gccggaaggc
     1681 gatctggatg cagtcccctc gccagatctc cttctcccgg tagctctggt cgaaatggtc
     1741 gtcggtgact tcggcagcca cgtagaggtt tttcgcatcc caggccacgc gcactttgcc
     1801 agcgagatcc gtcgttccct tccacccgtt caccttcgat tgtgcctgag cgcttacggc
     1861 gatgggctcc accccctgcc actcagagag gtctccatct agagtgggag gctgggcaaa
     1921 ccgcctggct gtagctgcag ggcgcaccgg cgccacggga acgcgccgct ccggttgggg
     1981 agtgacgagg tcggcgcgga ccgtcaccga gcccgcggca ccttcgggca cctggcagcg
     2041 taccgggaag gagagcacct cgcccggctc cagccggtcg atcaccagcg cacgcccggg
     2101 ctccacgctc caggatgccg gcagatgaag cagcaggcgc acgtcggtga ccggcttcgc
     2161 gcctccccag acgcacacgt tcagctcggt ggtctcgccg gcgcgcggcg gggtcacgaa
     2221 gcggacgtcg gtgcgcggtg cctgcgggag ctcctcgggg gttggcgctg cctggccgtc
     2281 gcgatcgatg cgtgcgcgaa tcgcggacgc ctgtccgggg cggacgggcg cgaggtccag
     2341 cacgcggatc tcgcgcgggg caagtgcctg agtcagcgat tcgtcgttcg ccgataccag
     2401 cgcgatgggg gacgccgcgt ggccttttcc ggctcctgga gtgagccggc gaacgaccca
     2461 ttgatcactg ccggatagtc cccacgcccc cggctcgaag cggaaagcgt agcgctggaa
     2521 gctatccgaa aggttggcta tcaggagtcc gacgtggcca tcgcgcgcgc gccacaccgt
     2581 tccgagtgtg gcgggtagcg ccgcgggcgc ctttccacgc ccggttgacc actcggcctc
     2641 cagcgaggcc aacggcgcca ccgggcggag ctccccgatc aactcgcctg tgaccacaaa
     2701 ctccgcggct accgcgcggt agcgcgccac ttcgcgcagg tactcggcgt tttcgcggtt
     2761 ggccggatcg agcagctcga agcccatcca gcctggctgg catccccaca ggaaatcacg
     2821 cccctgaacc atccggaagg caagcggctc catcgtccgc gccgggctgg agaagtagag
     2881 cgtgtagccg ctgtagacgg cggtcatcat cgggatctcg gcgtcgcggc gcgggttcca
     2941 gaccaggaaa gcatccacgt tatccatgta gggctcggcg ttgttctccg tggtgatgcc
     3001 caagcggcca gcagtttgag cacgtacgcg gtcgagcatc tcccggtagc catccaccca
     3061 atgccggcca ccgcccagcg ggtgcccatg cgtgggatcg aagcagagct gtggcctggc
     3121 cgcgccaatc tggtcgaggt agatggcatt cacaccacac tctcggatca ggcgtccgac
     3181 gatctcattc actttctcct gccacagctt tgtcgcgggg cacatcggcg cgagtttggc
     3241 gccactgcca tactcctcga tgtaggtcga ggtgccatcc ggctgcttgc acgcggcggc
     3301 acgccccacc gcgaagttat cattcgccgt atcccatagc cgaccgttga tgtagggcat
     3361 ggcgatgata ccctctttga caagctcgcg cacgccctcg gcaaacccgg gctttgtggg
     3421 gaaatagtcg ggatagtgga catcaaacgg gatctgatgc cagttgtacc agtggacgcc
     3481 catcggcacg ccgaacaggc gctttgcctc aagcatccgc ggcaccacct gctcgcgcgt
     3541 cccgcttgcg agcatccaga gaccgaggcg cttcatcgcc tctggcatat ctttgcgcgt
     3601 ggccaccggg ccgcgccggg tccacggctg tttcagcgcc cattcgcgat accgttttgc
     3661 cccctgccac cagtcgccct gatagacgcc aatcgccact gggaaaggcg cccggaaatc
     3721 ggacccaggt acccccatcc cttcgacggg agtggtgagg cgaaactcgc cgcccggcgt
     3781 gaaggcaaag tgcttggggc tggcgcccgg gtcatgcgcg gcgagataga ggccatgctc
     3841 cccctcgttg accagaagga actgcatcgg ccagtcggcg ctcgggtagg agccaaagat
     3901 ctgcgcgctg gcgctgcggt agaggtagcc ccagttgcct cgcggcaccg ccacgtccgg
     3961 catccccttc tcggagaggc gggcaaagag agggaagtgc acttcccaaa gcccataccg
     4021 ctcgctggag ttgtccacat cgatccgcca caacgacatt ccgggcgagg ccttgggcag
     4081 gtccactgtc acgcggacgc gcagcgcccc cttctcctcc ccggtatcga tgtcctccca
     4141 ggtgagctcc aggcgcttcc catccttcga gggcgtttca gcggcagagc accgcgccgc
     4201 cgccgtatta tcaatcctca aatcaggcag atcgcggccg cgtaggacca aacgccacac
     4261 caggggtttc tcggacggcg tgaggaactc gaggccggtc cgatggctga agaggctggt
     4321 ggcccccaac ccctcttctg gacgggcgaa gccaaggcgg aggctgccgc tcgcgatgct
     4381 caccggctgc ggcgaaggaa cgctccggtc atggccaccc ggcgctacat cctcgtgcac
     4441 ctgcagctgg ctctcttctg ccaggcgcgc ccggatctcc tccggcgcgc gcgccggccc
     4501 gaggtagacg cgggcgcgca gatcggcggc aggcgcccgg aaggggccca tcgtctgctg
     4561 gatatagttg taatagctgt tggcgccatc gtagagggaa accgtgccgt tgaggctcag
     4621 gccgatggca tcctcatcgt tggccatcat cgcccatctc gagagggcat agcctttccg
     4681 ggcatcggta aacaccccgg tctggagcgg ctcgccgccc gcccagcgcc cgaagctgct
     4741 atccttgcgc gaaatctgca agaagtggag ctcgctccag gtaaagtcgt catcgcgctc
     4801 aatacgggct gtgacctcca ctacgggcga ggaagcccgg taatgatagc gatagacggc
     4861 gcgcggattg cctggctcct tcgcggcctt cgcccgcgtt tcgatgacca cctgcaccgg
     4921 acccgcttca agcacgcgcg ctgtcgcctc ggcatccgag cgccacgcct gagagccgcg
     4981 cgtcttctcg aaaagccggt cctcgaaaac aaaccccttc tccaccactc ccgactggtt
     5041 gaaccggatg gaggtcggga agcccccgtt gccccgcttt ggatgctcga cccggtaata
     5101 gagattcgcg atcgtgatgg tgttggccgt ctccgtcacc tggaggtcgc tgcgcggagg
     5161 atccgcgtgc ttgcccgctt cgacaacgat ccggcgcgcc atccccgtgg aagtcgtccc
     5221 cggcaagagg acgaggacgc caccttcagc gggctgccag gcgagaggcg tcgcgccctc
     5281 tcgcacggcg gcaatacccc gcaggcgctc catgcgcaga ggcatgagca cgctcccctg
     5341 ccacacactc cggccggcgt tcaccacggt aaacgcggtc gtgggctcgg ctggaagtgc
     5401 ttggggcctc cccagtagtg ccatgagggt ggcaaacagc cacacagatc gtctca
//
LOCUS       DAOSCV010000181         5452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9453_length_5452_cov_3.425977, whole genome shotgun sequence.
ACCESSION   DAOSCV010000181 DAOSCV010000000
VERSION     DAOSCV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9453_length_5452_cov_3.425977"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLY56_05865"
     CDS             <1..231
                     /locus_tag="PLY56_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HOJ21040.1"
                     /translation="GSGNSPNVLRAVEYANRIGMRTFGVTGFNGGRLREMVRACLHVP
                     CDDMGVVEAVHAIFFHYLVDALRLRFQGEGDA"
     gene            320..1372
                     /locus_tag="PLY56_05870"
     CDS             320..1372
                     /locus_tag="PLY56_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ21041.1"
                     /translation="MTVERLQQILDQFARARVLVAGDFFLDKYLVIDAGLTERSIETG
                     LPAYQVVEKRVWPGGAGAVSANLRVLGTGTVQALGLVGRDGEGYELRMGLEALGIDTS
                     LLVETNACFTPTYTKPVWREAGTEREMNRLDIRSRHPCPPDVEQRVLLQLRESVATAH
                     AIVVVDQVPERNRGVITDAVRAVLTELARAHPDTVFIADSRMRAGEYPGLSVKPNRAE
                     AIRTVFGERRAEPTFEEVLAAGRELQARSGRPTFITLAEDGLLIFDGKRVWHAPALRQ
                     TGPVDPCGAGDSVMAGIALALCSGASALEAGIVGNVVASITVQQIGVTGTASRDEVLE
                     RFRAAGDAFLPRPVDG"
     gene            complement(1379..2977)
                     /locus_tag="PLY56_05875"
     CDS             complement(1379..2977)
                     /locus_tag="PLY56_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOJ21042.1"
                     /translation="MTTREMKVTERYLHLPVKNGAPLRRMRFLVEGSVEREFDIELAE
                     GAPDFWVFSDLSAFEGKWLRVEAEGLPDGSDALAALRQAPSVPDADTIYRERYRPQFH
                     FSPRRGWNNDPNGLVYHRGEYHLFFQHNPYGREWGNMHWGHAVSRDLVHWEELPVALY
                     PRRYGDWVYSGSAVVDTRNTAGFQRGLEDALVAIYTSTGRGECIAYSTDRGHSFTEYP
                     GNPVVRHRGRDPKVIWYAPGKHWVMAVYDEQGEGDALSKGIAFYTSPDLKVWKFASRV
                     EGYYECPELFELPVDGNPENTRWVLHAADGDYTIGHFDGQRFVAESGKHRFYHSDCFY
                     ASQTFNNIPCEDGRRIQIAWARAGHPEMPFNQMMGFPVELTLRTTEDGIRLFAWPVRE
                     IETLYRARHAWDHLTIPEGSLSLDGVSGELLDLSLELDPGSSTAAGASVHGVPVTYDC
                     VKRELSCLRWSAPLSPVNGRLRIRVLVDRLSLELYANDGRVYLPVCAVIPEGSQGVEL
                     FTRGGTAVATRVEIHELRSAWEPA"
     gene            complement(2974..4503)
                     /locus_tag="PLY56_05880"
     CDS             complement(2974..4503)
                     /locus_tag="PLY56_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21043.1"
                     /translation="MIALLALMAMTATESAEASSWPIRGLIENEDCTNFFYYRRFGEG
                     VDGGGMVDRYVDTLADAGVRVLMINTNARRTNYRSQAWDAFWDGLDPAGPDDQPFLKP
                     VPREGIAAYRRLVQSMRDLHAQGVDYPARMAARCRQRGISPWISLRINDVHNNDILDH
                     PFHGRFWVENRHLFRKGATGYFANALDYAHPEVRGLYLALIRESLDRYDIDGLELDFM
                     REPYVFSPGKEAEGAPVLTAWLRDVRGLVDEAAARRGHPVRLGVRVPSRPETAKALGL
                     DAIAWAREGLVDLVVPAPRWATLEFDMPIAEWRRLLRPFPKVMLAGGMEILYRPHPGA
                     KPVSVSPEHAHAAAAAIWEAGADAVYLFNYFQDDWRPETYRKLLLTLGSREKVAQAPR
                     WNAVTYRDIVGPGEEYRPPLPAEGTSLRFDLPVCPAPGRDWAVRVVLGLENAAQPPAI
                     RVNGEEGVLEKAEAHEAVYSVPSAAFAGRARAEIGVHAGQPVRVVKVEVRVTPPAEGE
                     R"
     gene            4732..>5452
                     /locus_tag="PLY56_05885"
     CDS             4732..>5452
                     /locus_tag="PLY56_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21044.1"
                     /translation="MRALVFAMLACIAPFLTLPCSGAPAREPLPLKPFTYVEDFEEPR
                     QMVVWATNGKQEIHFSGVTEEKAFSGKRSYKLDVTIREGAYHYFGIRLPRVPCEGELK
                     LSGRLWVEALPQGARAGLGYNIILPPSQHSGCAAVETIDRPTPEWKAVSGDLVAAGKQ
                     TATGVIGRHTAEMDGSEVAPFLELWSVPIWATAGQRITLYVDDVRIEGRVPDADAFAA
                     AAQERFTAARERTARRIAGWRA"
BASE COUNT          895 a         1849 c         1826 g          882 t
ORIGIN      
        1 ggttccggga acagccccaa cgtcctccgc gcggtggaat acgccaaccg cattgggatg
       61 cgcaccttcg gggtcaccgg gttcaacggc ggccggctgc gcgagatggt ccgcgcctgc
      121 ctgcacgtgc cgtgcgatga catgggcgtg gtcgaggcgg tccatgccat ctttttccac
      181 tacctggtgg atgccctgcg gctccggttt caaggagagg gggacgcatg agcagccggg
      241 tatcgccggg ccggcactct cccactcgca tcctccatcg ccggcgggcc agcaccgcga
      301 tctcgagagg tacgccttga tgacggtcga gcgtttgcaa cagatcctgg accagtttgc
      361 tcgcgcccgg gtgctggtgg ccggcgactt cttcctcgat aaatacctgg taatcgacgc
      421 cgggctcaca gaacgctcga ttgagaccgg gttgccggcg tatcaggtgg tggagaagcg
      481 cgtctggccg ggaggtgcgg gggctgtctc tgccaacctg cgcgtcctgg gcaccggcac
      541 ggtccaggcg ctcgggctcg tgggacgtga tggcgaaggc tacgagctcc ggatgggact
      601 cgaggcactt ggcatcgaca catccctcct ggtggagacg aacgcctgct tcacgcccac
      661 ctacaccaag ccggtgtggc gcgaggcagg gaccgagcgc gagatgaacc ggctcgatat
      721 ccgaagccgc cacccgtgcc cgccagacgt ggagcagcgc gtgctcctcc agctgcgcga
      781 gagcgtcgct accgcccatg ccatcgtcgt ggtcgaccag gtgccggagc gcaaccgcgg
      841 ggtgatcacc gatgccgtgc gcgcggtgct gacagagctg gcgcgcgccc atcccgatac
      901 ggtctttatc gccgattcgc gcatgcgcgc aggcgagtat ccgggcctct ccgtgaagcc
      961 aaaccgggcc gaggcgatcc gcacggtttt cggcgagagg cgggcagaac ccacgttcga
     1021 ggaggtgctc gccgccgggc gagaactcca ggcgcggagc gggcgtccca ccttcatcac
     1081 tctggcggag gatggtctcc tcatcttcga tgggaagcgc gtttggcacg cgccggcgct
     1141 tcgccagacg gggcccgtgg acccctgtgg cgcaggcgat agcgtcatgg ccgggattgc
     1201 cctcgccctc tgctcgggcg cgagcgcgct ggaagccggc atcgtcggca acgtggtcgc
     1261 gtcgatcacg gtccagcaga tcggcgtcac cggtaccgcg agtcgggacg aggtcctgga
     1321 gcgtttccgc gccgcgggcg atgcgttcct cccgcgcccg gtcgatggat aggcgtgcct
     1381 aagccggctc ccacgcggag cgcagctcgt gaatctccac ccgggtggcc acggcggtgc
     1441 cgccccgcgt gaacagctcg acgccctggc ttccctcggg gatgacggcg cagaccggaa
     1501 ggtagacgcg cccgtcgttc gcgtacagct cgagcgagag acggtctacc agcacccgga
     1561 tccggagccg gccattcacc ggcgagagcg gcgcgctcca gcgaaggcac gagagctccc
     1621 gcttcacgca gtcgtaggtg accggcacgc cgtggaccga cgcgcccgcc gcagttgagc
     1681 tgccgggatc gagctcgagc gagaggtcga ggagctcccc ggagacaccg tcgagagaca
     1741 gtgagccttc tgggatggtc aggtgatccc aagcgtgtcg cgcgcggtag agcgtttcga
     1801 tctcccggac cggccaggcg aagaggcgga tcccatcctc ggtggtccgc agcgtcagct
     1861 ccacggggaa gcccatcatc tggttgaacg gcatctccgg gtgcccggcg cgtgcccagg
     1921 cgatctggat ccgccggcca tcctcgcacg ggatgttgtt gaacgtttgc gaggcgtaga
     1981 agcagtcgct gtggtaaaag cggtgcttgc ccgattccgc gacaaaccgc tggccgtcga
     2041 agtggccgat agtatagtcg ccatcggcgg cgtgaagcac ccagcgagtg ttctcagggt
     2101 tgccatcgac gggcagctcg aacagctcgg ggcattcgta gtagccctca acacggctgg
     2161 caaacttcca caccttcagg tctggcgagg tgtagaaggc gatacccttg ctgagagcgt
     2221 cgccctctcc ctgctcgtca tacaccgcca tcacccaatg cttgccgggc gcgtaccaga
     2281 tcaccttcgg atcgcgcccg cggtgcctca ccaccgggtt ccccggatac tcggtgaagc
     2341 tgtgcccccg gtcggtgctg taggcgatgc actccccccg gccggtgctg gtgtagatgg
     2401 cgacgagagc atcctctagc ccgcgctgga aacctgcggt gttccgggtg tccaccaccg
     2461 cgctcccgga atagacccag tcgccgtagc gacgcgggta gagcgccacc ggcagctcct
     2521 cccaatgcac caggtcacgg ctcaccgcgt gcccccagtg catgttgccc cactcgcgcc
     2581 cgtaggggtt gtgctggaag aagaggtgat actcgccccg gtggtagacc aggccgttgg
     2641 ggtcgttgtt ccagcctcgc cggggagaaa agtggaactg aggtcggtag cgctcgcgat
     2701 agatcgtgtc ggcgtccggc accgaggggg cctggcggag ggctgccagc gcgtccgatc
     2761 catccggaag cccctccgcc tcgacgcgca gccactttcc ctcgaaagcc gaaaggtcgc
     2821 taaagaccca gaagtcgggc gccccctccg ccagctcgat atcgaactcg cgctcaacgc
     2881 ttccctcgac gaggaagcgc atccggcgca gaggcgcgcc gtttttcacc ggcaggtgga
     2941 ggtagcgctc tgtcaccttc atctcacgcg tggtcatctc tcgccctccg cgggcggggt
     3001 cacgcgcacc tccaccttta ccacgcgcac cggctgtccg gcgtgaacgc cgatctcagc
     3061 ccgtgccctc cccgcgaacg ccgcgctcgg caccgaatag actgcctcgt gggcctccgc
     3121 cttttccaaa acgccctcct ccccattcac gcggatcgcc ggcggctgtg ccgcgttttc
     3181 caggccgagg acgacgcgaa ccgcccaatc tcttcctggc gccgggcaaa ccggcagatc
     3241 gaagcggagc gacgttccct cggccggcaa aggcgggcgg tactcctcgc ccgggccgac
     3301 gatgtcacgg taggtgactg cgttccagcg cggcgcctgc gccaccttct cgcgcgagcc
     3361 cagggtcagc agcagcttcc ggtaagtctc cgggcgccag tcgtcctgga agtagttgaa
     3421 gaggtagacc gcgtccgcgc cggcctccca gatcgccgcc gcggcggcat gggcgtgctc
     3481 gggcgaaacg gagacgggct tcgcacccgg atgggggcga tagaggatct ccatgccgcc
     3541 cgccagcatc accttcggga agggacgcaa caagcggcgc cactccgcga tcggcatgtc
     3601 gaactccagc gtggcccacc ggggcgcggg caccactaga tcgaccaggc cctcgcgcgc
     3661 ccaggcgatc gcgtcgaggc ccaaagcttt cgccgtctcg ggccgggagg ggacgcgcac
     3721 gcccagccgc accggatgcc cccggcgcgc tgccgcctcg tccaccagcc cacgcacatc
     3781 ccggagccac gcggtgagca ccggggcgcc ctcggcctct ttgcctgggc tgaagacgta
     3841 cggctcgcgc atgaagtcca gctccaggcc atcaatgtca taccggtcga gcgactcgcg
     3901 aatcagcgcc aggtagaggc cacgcacctc gggatgggcg taatcgaggg cgttggcgaa
     3961 gtagccggtg gcgcctttcc ggaagagatg ccggttctcc acccagaacc ggccgtggaa
     4021 gggatggtcc agaatgtcat tgttatggac atcgtttatg cgcaggctga tccagggaga
     4081 gatgccccgc tgccggcagc gcgcggccat ccgggcgggg tagtccactc cctgcgcgtg
     4141 gaggtcgcgc atgctttgga ctaggcggcg atacgccgcg atgccctcgc gcggcaccgg
     4201 cttcaggaac ggctggtcat ccggacctgc ggggtcgagg ccatcccaga acgcgtccca
     4261 cgcctggctg cgatagttgg tgcgccgggc gttggtgttg atcatcagca cgcgaacgcc
     4321 ggcatcagcg agcgtatcca catagcggtc caccatgccg ccgccatcga cgccctcgcc
     4381 gaagcgacgg tagtagaaga agttggtgca atcctcgttc tcgatcagcc cgcgtatcgg
     4441 ccaggaagac gcctccgcgc tctctgtggc ggtcattgcc atcaacgcca gcaaagcgat
     4501 catcgcgaac atccctcctc tgccctcctt gggcgcacac cccttcgtgc ctcgcccgcc
     4561 tgaattcgtt ggcgggcatt atagccccct ggcggcgctt cgtcaaccgc gtgattacag
     4621 ggagggattc ttcgccggag gccgtatgat cccccataaa gagaccggcc cggttcatgt
     4681 ctgcgccgcg gtgcctggcc ccggacgaca ggagaaccac ggaggagagc tatgagagcg
     4741 cttgtcttcg ccatgttggc gtgcattgcc cccttcctga cactcccctg ctctggcgcc
     4801 cccgctaggg agccgctccc gctgaagccg ttcacttacg tggaggactt cgaggagcct
     4861 cgccagatgg tcgtgtgggc gaccaacgga aagcaggaga tccacttcag tggcgtgacc
     4921 gaggagaagg cgttctccgg gaagcgctca tacaagctgg acgtgacaat tcgcgagggc
     4981 gcctaccact acttcgggat tcgcctgccc cgcgtgccgt gcgagggaga gctaaagctg
     5041 agtggccgcc tctgggtaga agccctgccc cagggcgctc gggccggcct gggctacaac
     5101 atcattctcc cgcccagcca gcacagcggg tgcgccgcgg tggagacgat cgacagaccg
     5161 acgccggagt ggaaggcggt atccggggat ctggttgccg cagggaagca gacggccacc
     5221 ggcgtgatcg ggcgccacac cgcggagatg gatggcagcg aggtcgcacc cttcctggag
     5281 ctttggagcg tgccgatctg ggcgacggcg ggccagcgga tcaccctcta cgtcgatgat
     5341 gttcgcattg aggggcgggt gccggacgca gatgcctttg ccgccgcggc ccaggagcgg
     5401 ttcacggcgg cccgagagcg gacagcgcgc cggatcgccg gctggcgcgc cc
//
LOCUS       DAOSCV010000182         5441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9479_length_5441_cov_3.194207, whole genome shotgun sequence.
ACCESSION   DAOSCV010000182 DAOSCV010000000
VERSION     DAOSCV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9479_length_5441_cov_3.194207"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLY56_05890"
     CDS             complement(<1..434)
                     /locus_tag="PLY56_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21045.1"
                     /translation="MSEWANLVHELGMLARLERSGLHFLGSGRQSVAEHSHRVACIAL
                     ILARRCKEPVDEARLAWLCLFHDLPETRIGDHNYVNRKYLRVDMERLRADISTASPLG
                     KTLIATIDEFEQGDTLEARLARDADQIDLLALLREEADLGNPR"
     gene            718..1224
                     /gene="ruvC"
                     /locus_tag="PLY56_05895"
     CDS             718..1224
                     /gene="ruvC"
                     /locus_tag="PLY56_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008686995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOJ21046.1"
                     /translation="MLALRVLGIDPGLGTTGYGVVELVAGAPLIREAGTVRTRRGDAL
                     PERLRTLYEGISEVIRDCHPEVIALEDLYTEYQFPRTALLMAHARGAICLAAAHQAIR
                     VVSFAPSEVKSAVVGFGRASKEQVQGTVQRVLGLSQPPHPNDVADALALALTGCYREG
                     SPLQARER"
     gene            1221..1874
                     /locus_tag="PLY56_05900"
     CDS             1221..1874
                     /locus_tag="PLY56_05900"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HOJ21047.1"
                     /translation="MIGYLRGNLVELEDERVILCVGGVGYEVILPAIEMRALRARLGE
                     ALYDQERLRAQEVELYIYEHIADRQPNTLFGFNQRLEKEFFRLLITVSDIGPATAAKA
                     MAIPVAEIAAAIERRDTRVLSSLPGIGKRKAEQMVATLKGKSLPFCLAPIPDLEEAPP
                     PEDVSQFVKDVQQILVEQLGYKPHEAEAMVTRALQRRPELTDAQALLEEIWTGERPS"
     gene            1880..2908
                     /gene="ruvB"
                     /locus_tag="PLY56_05905"
     CDS             1880..2908
                     /gene="ruvB"
                     /locus_tag="PLY56_05905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ21048.1"
                     /translation="MARERLITNEELPEDQAATVALRPRSFDDYIGQQEVVRPLRIAL
                     EAARQRGEPLEHVLLHGPPGLGKTTLAHIIRHYMGVEVIATSGPALKRPGDLMGLLTN
                     LKEGDVFFIDEIHRLDPAVEEYLYPAMEDFIVDFVVDKGANARMLNFKLRRFTLVGAT
                     TMAGLLTAPLRDRFGLVYRMNYYTPDELRAVILRSAKLLRIEIGEDAAAEIARRSRGT
                     PRIANRLLRRVRDYAQVEAGGVITREVADAALRLEGIDNAGLDNLDRALLRTIIDVYQ
                     GGPVGIEALAATLNESQSTLVEVVEPYLLQIGFLARTPGGRKATLAAYQHLHVPTSGQ
                     AGQPNLGL"
     gene            3219..4154
                     /gene="trxB"
                     /locus_tag="PLY56_05910"
     CDS             3219..4154
                     /gene="trxB"
                     /locus_tag="PLY56_05910"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOJ21049.1"
                     /translation="MPDDVRDVIILGSGPAGLTAAIYTARAGLSPLVIAGDQPGGQLT
                     ITTEVENWPGMREGIMGPQLMEDMRYQAERFGTEFIDERATEVDLQGRPFQVYVGPDI
                     HLCKTLIIATGASARLLGLDSEAKLMGHGVSACATCDGFFFRDRDVMVVGGGDTAMEE
                     AIFLTRFAKHVTVVHRRDTLRASKFMQDRAMKNPKIGFIWDSVVDHILDVDQGKVTGV
                     RLRNVKTGELSEHAIDGLFIAIGHRPNTGLFEQQLELDAKGYIVTHDGTRTSVPGVFA
                     CGDVQDPVYRQAVTAAGSGCMAAIDAERFLEAEEG"
     gene            4286..4531
                     /locus_tag="PLY56_05915"
     CDS             4286..4531
                     /locus_tag="PLY56_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOJ21050.1"
                     /translation="MSTREITTCYLRSLKHTPHIADRLKSAYPVIVNVAEMPEEERVQ
                     ALDFISGVTYAVDGSYDQVGDNTFLFVPGNVILVDDE"
     gene            complement(4557..5033)
                     /locus_tag="PLY56_05920"
     CDS             complement(4557..5033)
                     /locus_tag="PLY56_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21051.1"
                     /translation="MSFARAASVGCGGVLAFFGAGIIGGTALSFGKEGATLGDLVMML
                     LFGLLPFALGTYWVTQVLKAHRKEAWEALEREVLSYAMRKGGVVTALEIAAHTNLSYD
                     EVKRYLDGLADKGHIEVTPTDNGLAYRFPGADTNPGAPLWSPPSTEAPHLPNRTSE"
BASE COUNT          930 a         1785 c         1748 g          978 t
ORIGIN      
        1 cgtggattgc ccaggtcggc ctcttcgcgc aggagtgcaa ggagatcgat ctggtcggca
       61 tcgcgcgcca ggcgtgcttc cagcgtgtcg ccctgctcaa actcatcaat cgtggcgata
      121 agcgtcttcc ccaaaggcga cgcggtgctg atgtcggcgc gcagtcgctc catatcgacg
      181 cgcagatact tccggttcac gtagttgtga tccccgatcc gtgtctccgg caggtcatga
      241 aagaggcaga gccaggccag ccgggcttca tccaccggct ccttgcaccg gcgcgcgagt
      301 atcaaagcaa tgcacgccac ccggtgggaa tgctccgcga ccgactgccg gccgctcccg
      361 aggaagtgta gcccgctccg ctccagccgc gccaacatgc ccaattcatg caccagattc
      421 gcccattcgc tcatgcggaa ctcctatctc tcgtcttcac tgccgccagt ataccaatcc
      481 ccgcgcctca gatcaacgct cgacgccgcg atgccgcgcg ggccgggcgc atcgcgcagg
      541 catggacatc cgtgccgcct gtggggaccg gaggtgcccg caccttcccc atccgtgtcc
      601 gcccccttca tccgtggcat cgctgccggt gttgggccgg ctgctgggaa aacctttgct
      661 ttgacgcagc aaaccgatgt atgctataat gcgatcgcga gcagagagcg agagggggtg
      721 ctggcgttgc gtgtgcttgg gatcgacccg ggtctgggga ccaccggcta tggcgtcgtg
      781 gagctcgtcg cgggcgcgcc cctgatccgc gaggcgggaa cagtgcgcac ccggcgcggc
      841 gatgcgcttc cggagcggct gcgcacgctc tacgagggca tctccgaggt gattcgcgat
      901 tgccatcccg aggtgatcgc gctggaggat ctgtacaccg agtatcagtt tccgcggaca
      961 gcgctgctca tggcacacgc gcgcggcgca atctgcctgg ctgccgcgca ccaggcgatt
     1021 cgcgtggtca gctttgcgcc cagtgaggtg aagagcgccg tggtgggatt tgggcgggca
     1081 agcaaggagc aggttcaggg cacggttcag cgcgtccttg gtctcagcca gccgcctcac
     1141 cccaacgatg ttgccgatgc gctcgctctg gcgctcacgg gatgctaccg ggagggcagc
     1201 cccttgcaag cgagggagag atgatcggct atttgcgcgg caacctggtg gagctggaag
     1261 atgagcgcgt catcctctgc gttggcggcg tgggctacga ggtgatcctt cccgccatcg
     1321 agatgcgcgc cttgcgcgcc cgcctgggcg aggcgctgta cgaccaggag cgcctgcgcg
     1381 cacaagaggt ggagctctac atctacgagc atatcgccga ccgccagccg aacaccctct
     1441 tcggtttcaa ccagcgtctg gagaaggagt tcttccgcct cctcatcacc gtcagcgata
     1501 tcggccccgc gacggccgcc aaggcgatgg caatcccggt ggcggagatc gctgcggcca
     1561 tcgagcgccg cgacacgcgc gtgctcagca gccttcccgg cattggcaag cgcaaggcgg
     1621 agcagatggt cgccaccctg aagggaaagt cgctcccctt ctgcctggcc cccattcccg
     1681 acctggaaga ggcgcccccg ccagaggatg tgtctcagtt cgtgaaggac gtgcagcaga
     1741 tcctcgtgga gcagctcggc tacaagccgc atgaggcgga ggcaatggtc acgcgcgcgc
     1801 tccagcgccg tccggagctg acagatgccc aggcgttgct ggaggagatt tggaccggcg
     1861 agaggccgtc gtaagagcca tggcgagaga gcgccttatc accaacgaag agttgccaga
     1921 agaccaggcc gcgacagtgg cgctgcgacc tcgctcgttc gatgactaca tcgggcagca
     1981 ggaggtcgtc cggccgctgc gcattgctct ggaagccgcg cgccagcgtg gcgagcctct
     2041 cgagcatgta ctgctgcacg gtccccctgg cctggggaag acgacactgg cgcatatcat
     2101 ccgccactac atgggtgtcg aggtgatcgc tacctctggc cccgcgttga agcgcccagg
     2161 cgacctgatg ggcctgctta ccaatctgaa ggagggcgac gtcttcttca tcgatgagat
     2221 tcaccgcctg gatccggcgg tggaggagta cctctatcct gcgatggagg acttcatcgt
     2281 tgacttcgtc gtcgataagg gcgccaatgc ccggatgctc aacttcaagc tgcgccggtt
     2341 caccctcgtc ggtgccacga cgatggccgg gctgttgacg gcgccgctgc gcgaccggtt
     2401 tggcctcgtc taccggatga actactacac cccggacgag ctacgcgccg tgatcctccg
     2461 ctccgcgaag ctgttgcgga tcgagatcgg ggaggatgcc gcggcggaga tcgcccgccg
     2521 gtcgcggggt accccccgca tcgccaaccg cctcctgcgc cgcgtgcgcg actatgccca
     2581 ggtggaggcc ggtggcgtga tcacgcgcga ggtcgcggat gccgcgctcc gcctggaggg
     2641 gatcgacaac gccggtctgg acaacctgga ccgcgccctc ctgcgcacga tcatcgatgt
     2701 ttaccagggc gggcccgtgg ggatcgaggc gctcgccgcc acgctcaacg agagtcagag
     2761 caccctcgtg gaggtcgtcg agccctacct gctccagatc ggcttcctcg ctcgcacccc
     2821 gggcgggcgc aaggctacgc tggccgccta ccagcacctg cacgtgccca cctctggcca
     2881 agccggtcaa ccgaacctgg gcctgtaacc cggcgcgttc gctccacgat cacgcgccgg
     2941 cgtctcctcg cgcacgcctg cggggccgcg ctgctccccg acgaaggccc ttcgggttct
     3001 ccatgcccca ccccgcgagc tcttgttgtc cgagcacatc gcttactcgg cccggtaggg
     3061 ctttgaatgc aagcggcgcg ggtaccaacg cccgcgcgac gcgcgtagcg cgaatcgtta
     3121 ccgtcatccc ttccagggct cgcgcggcgt gtacctcctc cttggcaaac cgctcgttct
     3181 cgtgtatact tagacttggt gaatgggagg aacacgacat gccggatgac gtgagggacg
     3241 tgattatcct gggctcgggg ccggccggac tcaccgcggc gatctacacc gcgcgcgccg
     3301 gcctttcccc cctggtcatc gccggtgacc agccgggtgg gcagctcacg atcaccaccg
     3361 aggtggagaa ctggccgggc atgcgcgagg ggatcatggg cccgcagctc atggaggaca
     3421 tgcgctatca ggcggagcgg tttggcaccg agtttatcga cgagcgtgcc accgaagtgg
     3481 acctccaggg ccgccccttc caggtctacg ttggtcccga tatccacctg tgcaagacgc
     3541 tgatcatcgc cacgggcgcc tcggcccggc tgctggggct cgactccgag gcgaagttga
     3601 tgggccatgg cgtctcggcg tgcgcgactt gtgacggttt ctttttccgt gaccgcgacg
     3661 tgatggtggt tggcggcggc gacacggcga tggaagaggc gattttcctc acgcgctttg
     3721 cgaagcatgt caccgtagtg catcgccggg atacactgcg cgcctccaag tttatgcagg
     3781 atcgcgcgat gaagaatccc aagatcgggt tcatatggga ttccgtcgtc gatcacattc
     3841 tggatgtgga tcagggaaag gtgaccggcg tccgcctccg caacgtgaag acaggcgagc
     3901 tcagtgagca cgcgattgat ggtctcttta tcgccatcgg ccaccgccca aataccggcc
     3961 tgttcgagca gcagctcgag ctcgatgcta agggatatat cgtcacccac gacgggacgc
     4021 gcaccagcgt tcctggggtc tttgcgtgcg gcgatgttca ggacccggtc taccgccagg
     4081 cagtcaccgc ggcgggatct gggtgcatgg cagccattga tgccgagcgc ttcctagagg
     4141 cggaggaagg atagcgccgg ggttctggtc cggcagccgg cggtcctcat agcgggagtg
     4201 gatggtctgg cggttctgtg attgcccggg cgcgcgttga cgcggggccg gtgatgtggg
     4261 ggttattttt gtgcgcaagc gagaggtgtc tacgcgagag attacgacct gctaccttcg
     4321 ctccctgaag cacaccccgc atattgccga ccggctgaaa tcagcgtacc ccgtgatcgt
     4381 caacgtcgcg gagatgcccg aggaggagcg cgtgcaggcg ctcgacttca tcagtggggt
     4441 gacctacgca gtggatggct cctacgacca ggtgggtgac aacaccttcc tgttcgtgcc
     4501 cggcaacgtc atcctcgtgg atgacgagtg acctctcgtt cgctactctg cggccttcac
     4561 tctgaggtgc gattgggcag atggggtgcc tccgtggagg gtgggctcca gagaggggcg
     4621 cccggattgg tatcagcccc tgggaagcga taggccaaac cgttgtcggt tggcgtcacc
     4681 tcgatgtggc ccttatctgc caggccatcg aggtagcgct tcacctcatc gtacgagaga
     4741 ttcgtatgcg ccgcgatctc cagcgcggtc accacgccgc ctttccgcat cgcgtagctc
     4801 agcacctcgc gctcgagagc ctcccaggct tcctttcgat gagccttcag cacctgagtg
     4861 acccaatagg tgcccagagc aaacggaagc aagccgaaga ggagcatcat gacgaggtcg
     4921 ccgagggtgg ccccctcctt gccaaaggag agcgccgtcc ccccaatgat accggctccg
     4981 aagaacgcga gtactccgcc acagccaacg ctggctgcac gcgcgaacga catcccctgt
     5041 ctcccgggca tcactcaatc ccttcacaag cgtctcaaac ccacgctgct ccgtgatatc
     5101 catcggcact ctgccagagt gggttgaggc agccatgcca gcggtcagaa gcgcccctcg
     5161 caccctgacc gttgtacaga ttcacggctg gtcacgcctg ctccttctct gggaaagcgg
     5221 gaatgccccc ctgcccgact gtccacaagg aacgaagcgt cttcctgtcg ggcgccccct
     5281 cacgctccgc catcacggag cgatctcatc cgcaggggag cataccggca gcttccaagg
     5341 cgagggccac cgcgccgcgc acgccggcat cgtagccgtt ctcggccggc aggatgcggg
     5401 ccgcgcgata ggggtattcc atgcaatagt cgcgcacgcg t
//
LOCUS       DAOSCV010000183         5438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9487_length_5438_cov_3.841167, whole genome shotgun sequence.
ACCESSION   DAOSCV010000183 DAOSCV010000000
VERSION     DAOSCV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9487_length_5438_cov_3.841167"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /gene="fliF"
                     /locus_tag="PLY56_05925"
     CDS             complement(<1..1224)
                     /gene="fliF"
                     /locus_tag="PLY56_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOJ21052.1"
                     /translation="MRERWDQLPGQRQKTLVLLALISVVTALGILAWAFQPRYSRLYS
                     QLSVEDAGAIVERLKSEKIPYRISDGGSAIEVPSEQVHELKMKMATEGLPRSSGPGWE
                     LFDKSPFGMTEFTQKLNFQRALQAELAKTIMQLSQVEQARVHLTIPEPSLYQEDAEEP
                     TASVVLHLSGTKPLAKNEIKGIVHLVSSAVVGLKPENVTLIDARGNILSTEEVMADED
                     PDATPDQTETQLKLQAQIERKVQREIQSMLDKALGPEQAVVRVRAQMDFTRRESNTET
                     YTPAVQGQDAKGVLNEQHTVEENYSGRRNAGQVPGAASNIQGMLPGMVGYRAEDTSPG
                     AGYVRRETTARYHVSKKVERLVSHPGETIRMSVAILVNGPIDEAKREALRDAATAAAG
                     LDIARGDQISVVGIAF"
     gene            complement(1341..1679)
                     /gene="fliE"
                     /locus_tag="PLY56_05930"
     CDS             complement(1341..1679)
                     /gene="fliE"
                     /locus_tag="PLY56_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOJ21053.1"
                     /translation="MRIAFPGDSLIRVPVPDPVERVQPGLPLGEAARPGAADETRSFS
                     EMMRDSLNEVNRLQLEVDDLSEKVSTGSVEDLHTAMIAVQKASLALELTLQVRNKAIE
                     SYQEIMRMQL"
     gene            complement(1676..2065)
                     /gene="flgC"
                     /locus_tag="PLY56_05935"
     CDS             complement(1676..2065)
                     /gene="flgC"
                     /locus_tag="PLY56_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOJ21054.1"
                     /translation="MRLFTAFEISGSALSAQRVRMDTIATNLANAESTRTPEGGPYQR
                     KMVTFREVLNEGVAVAGVVTDPTPGPRVYEPAHPDADAQGYVQLPNVQVAIEMVDMIS
                     ATRAYEANVSALTAAKRMALKALEIGQ"
     gene            complement(2067..2447)
                     /gene="flgB"
                     /locus_tag="PLY56_05940"
     CDS             complement(2067..2447)
                     /gene="flgB"
                     /locus_tag="PLY56_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOJ21055.1"
                     /translation="MGFLQDATWGALERSLDAAAVRQRAIAQNMANIDTPGYKRSVVR
                     FEEALARALDTPGNPDERARKVSSVMPRVERIGTSLRADGNNVDIDLEGAMMAENALH
                     YEAVSRMTSARVRLLRTAIMEGRR"
     gene            complement(2786..4168)
                     /gene="rpoN"
                     /locus_tag="PLY56_05945"
     CDS             complement(2786..4168)
                     /gene="rpoN"
                     /locus_tag="PLY56_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOJ21056.1"
                     /translation="MDQTLSLRQEMSVRMIPEQIVASMILRMNAEELRNHLEEELENN
                     PALDLRETVADTTTPFTCPFPTTPVLETPSRWRERASDDEESDLTADLPAHETLRDHL
                     WRQFVTVSLPEERAVGRYLIECIDDNGYLSVPLLEVAEELGKSLDAVEAVLHIIQALD
                     PPGVGARDLRECLLLQLRAGNWTEHRDAALAGEILERGWDLLVRQDFEAMSRHLGYSE
                     QRIQEGYAFIRRYLVPYPGRGVRPPWEMNAQEAPLIPDVCVRRTSAGYRVEVTDIDLQ
                     ASSLCINREYQNICRAMQRGKGTFSEDERSHVQEALERARFLLKGLARRARTLQRVTE
                     YVIAQQRAFVEGDAQALRPLTRRDVALQMGLSESTVCRATIGKCVELPSGSIVPFEVF
                     FDVAGAIKRRIEALLRAEDPAAPLRDDDIADRLRADGIDIARRTVSKYREELGVPAYA
                     ERRRRYRASR"
     gene            complement(4580..>5438)
                     /locus_tag="PLY56_05950"
     CDS             complement(4580..>5438)
                     /locus_tag="PLY56_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21057.1"
                     /translation="GALPESSAELRAPRVRAAFSLCRNPRPARRMVDARPGMGYNAHM
                     QGDVGAAGGRQSGLAAMDADQLLQRFTRCLVHNMSNPLAAILGCGQLLERMLQDGEPV
                     DAGQCRAYVSMILSETEKCARALERLALLARPPELRPIPTDLHELIQRALDAFEVPDG
                     ISFALEREAWDPTVIADPELTCHVLVNLLQNAAEAMPEGGTITVRTENCHEGEKWVRL
                     VVADEGPGIAPDLLPRITDPFFTTRQRHNGIGLTLCAQFISRQNGRLRIENGSPTGVR
                     AIVVLPQED"
BASE COUNT          940 a         1790 c         1671 g         1037 t
ORIGIN      
        1 aaacgcgatt cccacgaccg agatctgatc gccccgagcg atatccaatc cggccgccgc
       61 ggtcgccgcg tctcgcaatg cttcgcgctt cgcttcatcg atcggtccgt tgaccagaat
      121 cgccacggac atccgtatcg tctcgcccgg atggctgacg agacgctcca ctttcttcga
      181 aacgtgatag cgtgccgtcg tctctcttcg gacgtagccg gcgccagggg atgtgtcctc
      241 ggcccgatag ccgaccattc cgggaagcat cccctggatg ttcgaagcgg ctcctggcac
      301 ctggcccgcg ttccggcggc cggagtagtt ctcctcaacg gtgtgctgct cgttgagcac
      361 cccttttgca tcctggcctt gtaccgcggg cgtgtaggtc tccgtgttgc tctccctgcg
      421 cgtgaagtcc atctgagcgc gcacgcgcac cacggcctgc tctgggccga gggccttatc
      481 cagcatgctc tggatctccc tctgaacctt gcgctcgatc tgcgcctgga gcttgagctg
      541 cgtctcggtc tgatccgggg tggcgtccgg atcctcgtcc gccatgactt cctcggtgga
      601 gaggatgttg ccgcgcgcgt cgatgagagt cacgttctct ggcttcaacc cgacaacggc
      661 gctggagacg aggtgaacaa tccccttgat ctcgttcttc gccaggggct tcgttcccga
      721 aaggtggagc accaccgagg ccgttggctc ctcggcatcc tcctggtaga gagatggctc
      781 cgggatggtc aggtggacgc gcgcctgctc tacctgggag agctgcatga tcgtcttggc
      841 gagctccgcc tgaagcgcac gctggaagtt cagtttctgc gtgaactctg tcatcccgaa
      901 ggggctcttg tcgaaaagct cccacccagg cccgctgctc cgtggcaggc cctcggttgc
      961 catcttcatc ttcagctcgt ggacctgctc ggagggcacc tcgatggcgc tgcccccatc
     1021 gctaatacgg tatgggatct tctcggattt caggcgttcg acaatcgcgc cggcatcctc
     1081 gacgctgagt tggctataga ggcggctgta gcgtggttga aacgcccacg cgaggatccc
     1141 aagggccgta accacgctaa tgagcgccag cagaaccagg gtcttctgcc gttggcccgg
     1201 aagctgatcc caccgctctc tcaagcggct tgcgttctct ttgaaggcat ccgacatggc
     1261 tgttgatgaa gactttcccc tgccgacggc gttagaccct gatcctgcct gagcctactc
     1321 gcccgcaccg ccctcggcgc ttagagctgc atgcgcatga tctcctggta cgactcgatc
     1381 gccttgttcc tcacctggag cgtgagctcc agggcaaggc ttgctttctg caccgcgatc
     1441 atggcggtgt gcagatcctc cacactgccc gtgctcacct tctccgagag gtcgtctacc
     1501 tccagctgga gccggtttac ctcgttgagg gaatccctca tcatctcgct gaaggagcgc
     1561 gtctcgtcag cagcgccagg ccgtgctgct tcaccgagcg gcaatcccgg ctgcacgcgc
     1621 tccaccgggt ctggtaccgg cacccggatc agggagtcgc caggaaacgc gattctcatt
     1681 ggccaatctc cagcgccttc agcgccatgc gcttggcggc ggtcaaagcg gaaacgtttg
     1741 cctcataggc acgtgtcgcc gagatcatgt cgaccatctc gatcgccacc tggacattgg
     1801 gcagctgaac atagccctgc gcgtcggcgt cgggatgcgc cggctcatac acgcgtggcc
     1861 ccggggtggg atccgtgacg accccagcca ccgccacccc ctcattgagc acttcgcgga
     1921 aggtcaccat cttgcgctgg tagggaccgc cctcgggagt gcgcgtgctc tccgcgttcg
     1981 ccaggttcgt cgcgatcgta tccatgcgga cgcgctgggc agagagagcc gagccgctga
     2041 tctcaaatgc ggtaaacaga cgcacgctaa cgcctccctt ccatgatcgc ggtgcgcaag
     2101 aggcgcaccc gagccgatgt catgcgcgac accgcctcgt agtggagggc gttctcggcc
     2161 atcatcgcgc cctccaggtc aatatccacg ttgttgccat cggcgcgcag tgatgtcccg
     2221 atccgctcca cccttggcat caccgatgac accttacgcg cgcgctcatc tggattgccc
     2281 ggcgtgtcga gggcgcgcgc cagggcttct tcgaaacgga ccactgaacg cttgtagcca
     2341 ggcgtgtcga tgttggccat gttttgggcg atggcccgct gccggacagc cgccgcatcg
     2401 agcgaccgct ccagcgcgcc ccaggttgca tcctgcagga atcccatggg cacctccatc
     2461 caacgttagc tggcccccaa gggggcgatg gacccgcaaa ggtattccgc aagcaggatg
     2521 ccgcgaatcg atctccgggg ccagaacgcc aggaataccg cctctactgc agtccgagtg
     2581 gctgcaaaga gcgccaacgt tcgctgagac ggaggcggca ctccccttcg ggtgtggggt
     2641 gtttcgtctc gcggacggtc gactggccca aggtgggaaa gcccgagcgc gccagatgcc
     2701 agacaaggac tcgcatggag tagcatggct ttggggagaa tcgggacatc ctcaaggcat
     2761 ccgctgcggt tttttgcgcc agggttcagc gggaggcgcg ataacggcgc cggcgctcgg
     2821 cgtaggcggg aacacccagc tcctcgcggt acttgctcac cgtacgccgg gcgatgtcaa
     2881 tgccatcggc tcgcaaccgg tccgcgatat catcgtcccg gagcggcgcg gcaggatcct
     2941 cggcgcgcag cagcgcctca atccgccgct tgatggcgcc ggccacatcg aagaagacct
     3001 cgaacggcac aatgctaccc gaggggagct cgacgcactt gccgatggtc gcacgacaga
     3061 cagtggactc gctcaggccc atctggagcg cgacgtcgcg tcgggtgaga gggcggaggg
     3121 cctgcgcatc accttcaaca aaggcgcgct gctgggcgat cacgtactcc gtcacccgtt
     3181 gcagcgtgcg cgcgcgccgc gccaacccct tgagcaggaa tcgtgcgcgc tcgagcgcct
     3241 cttgcacatg gcttcgctcg tcctcgctga acgtgccctt cccgcgctgc attgcccggc
     3301 agatattctg atactcgcgg ttgatgcaca gagaggaggc ctgcaggtcg atatcggtca
     3361 cttccacccg gtagcctgcg ctcgtgcgcc gcacgcacac atccgggatc agcggggctt
     3421 cttgggcgtt catctcccag ggcggccgca ccccccgccc cgggtagggc accaagtacc
     3481 ggcggatgaa ggcatagcct tcctgtattc tctgctcgct atagcccaaa tggcgcgaca
     3541 tcgcctcgaa atcctggcgg accaagaggt cccaaccgcg ctcgaggatc tcgccggcga
     3601 gggcggcatc ccggtgctcc gtccaattac cagcgcgcag ctgaagcagc aggcactcgc
     3661 gcaggtcgcg tgctccgacc ccgggcgggt ccaacgcctg aatgatatgc agcactgcct
     3721 cgaccgcatc caggctcttg cccagttcct cggccacctc gaggagaggc acgctcagat
     3781 agccgttgtc gtcgatgcac tcgatcagat agcgccccac agcgcgctct tcgggcaagc
     3841 tgacggtaac gaactgacgc cagagatggt cgcgcagcgt ttcatgggcc ggcagatctg
     3901 cggttaaatc ggactcctca tcatcagacg cccgctccct ccaccgggat ggcgtttcca
     3961 gcacgggggt ggtaggaaaa gggcacgtga agggtgtcgt cgtgtcggca acggtctcgc
     4021 gcagatccaa agccgggtta ttctctagct cctcttccag atggttgcgg agctcctccg
     4081 cgttcatgcg gagaatcatg ctcgcaacga tttgctccgg gatcatccgg acagacatct
     4141 cttgccggag gctgagcgtt tgatccacca cggaactccc acgttactcg cgtcgtcata
     4201 atagtgtctg cctctggtcc cgctccattc gtgtgggggg gtggcctcgt tactgaggcg
     4261 gcagatgtgc cttctcagat gaatgttggc ggcgcttaag cgccgggcga agccagccga
     4321 acgatgacac ggaggccgtt gaggacctct caggatggtt cgaggtggcg gctcctcaag
     4381 ccatatcagg ccacccgtgc agtatgccca cagcgagcag aaggccggtt cagggtcaca
     4441 ggaatcgcga cgctggggat tgccaccaca gcgccagctc ttgacgcgag cgaatgatgc
     4501 aggccgaaca atcggcccgg cggcatcctg gccccattag ctacctactg caatctccgt
     4561 gccgcgtttt tcgaggcgtt caatcctcct gcgggagaac aacgatcgcg cgtaccccag
     4621 tgggggatcc attctcgatc cggaggcggc cgttctggcg tgagatgaac tgcgcgcaga
     4681 gggtcagtcc gattccgtta tggcgctggc gggttgtgaa gaacggatca gtgatccgcg
     4741 ggagcagatc cggggcaatg cctgggcctt catcggccac caccaggcgc acccacttct
     4801 cgccctcgtg gcagttctcg gtccggaccg tgatcgtgcc gccctccggc atcgcctcgg
     4861 cggcgttctg gaggaggttg accagcacat ggcacgtcag ttccggatcg gcgatcaccg
     4921 tgggatccca cgcttcccgc tccaaggcaa aagagatccc atctggaacc tcgaaagcat
     4981 cgagcgcccg ctgaatgagc tcatggagat cggtgggaat cggccgcaat tcgggcgggc
     5041 gcgcaagcag cgccagccgt tccagggcac gagcgcattt ctctgtttcc gagagaatca
     5101 tgctcacata cgcccggcac tggccggcat cgacgggctc gccgtcctgc agcatgcgtt
     5161 cgaggagttg cccgcagcca agaatggcgg cgagcggatt gctcatgttg tggacaaggc
     5221 agcgcgtgaa acgctggagg agctggtccg catccatggc agccagacct gattggcggc
     5281 cacccgctgc cccgacatcg ccttgcatgt gtgcattgta gcccattccc ggacgcgcgt
     5341 caaccatgcg tcgggcgggc ctgggattcc gacataagga gaaggccgcc cgcacgcgcg
     5401 gagctcgaag ctccgcgctg ctttccggca aagcccct
//
LOCUS       DAOSCV010000184         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9492_length_5436_cov_3.653038, whole genome shotgun sequence.
ACCESSION   DAOSCV010000184 DAOSCV010000000
VERSION     DAOSCV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_5436_cov_3.653038"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLY56_05955"
     CDS             complement(<1..1087)
                     /locus_tag="PLY56_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOJ21058.1"
                     /translation="MQTLRIGLYQMNAWVGDLDRNVQTILTALEEARARGVDVVAFPE
                     LAITGCPPDDLLLRPRFLQENRRALERVAAATTGLTAIVGFVDFQESLFNAAAVLHNG
                     HLAGVYHKRVLSRSGAFDETRYFQPGEETRVFSLGGVTFGVTIGEEGWAPDGPASTQC
                     LLGGAQVVFNLSAAPFVMGQRPARERRLAIRAAEDACFLAYLNGTGGQDELVFDGGSV
                     LLGPDGEMLLRAPVFAETLVVADLDLSSVTRARLRDPSWGQRRRARQAGGEAVERVIL
                     EPVALRPDRPPVAAESAPVRDRLEEVYTALVTGTRDYVTKNGFKDVVIGLSGGIDSAM
                     VACIAVDALGKERVVGVNMPSVYSSEET"
     gene            1286..2581
                     /locus_tag="PLY56_05960"
     CDS             1286..2581
                     /locus_tag="PLY56_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21059.1"
                     /translation="MSQSRDTRADAPAIALSGSPKAVGTLWGETNRSTIHNDLESLFL
                     APARERGLTTDVLIARSRRFVEIAEQLAPHWIEEAHSIARAAGVEGELYLAFIAGVYR
                     GLFLHEEESAPDPAASEDECTSYTIAPAETAGNAILFHKTRDNVARAQSAFVLRSTSP
                     GIQAFLAVSDASVLAAMMMVNERGLAGSADMMGLDEVRPRFRGMMNTFILRHIAECAS
                     CCAEALEIIREFVSRGWYAGGAKTGTHWLFVDREGTALEVSNNAVEVQSRLHPEGVYF
                     SLRADTPGAEALRQAPRPVEFTTFHNVSRHPSLCLETSISGMTVEIDRTCPEKLTTAW
                     VTFPARSLAFPLFMGIRETPHALIDGSVDRAGQAVTGEIKRYEEMEQAIYARQQAIRE
                     EARALLTSGHAAKVEELLDTWVQRQAMENLAALQELARA"
     gene            complement(2698..3693)
                     /locus_tag="PLY56_05965"
     CDS             complement(2698..3693)
                     /locus_tag="PLY56_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOJ21060.1"
                     /translation="MPLMTTKPMFDLAYDGGFAIGAFNVNNMEILQGIIEAAKEERAP
                     LILQISKGAREYAKMVYLRALINAAVEDSGDLPIAIHLDHGDSVELCKQCVDDGFTSI
                     MIDASKHPFEENIRITKEVVDYCHPRGVTVEAELGQLGGIEEHVVGVDDPMKHLTDPN
                     QVAEFWQRTGCDSLAVAIGTSHGAYKFKSAPKLAIDRVSEIMEKAPGLPLVMHGSSSV
                     LQEFVEEINKYGGNMPNTMGVPESAVTQVSQMGVCKVNIDTDLRLALTAKIRKTFAEK
                     PAEFDPRKYLGPGREAIKEMVKHKLHVLGVAGKADAVIDQWKKLGCPVPDYYKNK"
     gene            complement(3817..5403)
                     /gene="pckA"
                     /locus_tag="PLY56_05970"
     CDS             complement(3817..5403)
                     /gene="pckA"
                     /locus_tag="PLY56_05970"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOJ21061.1"
                     /translation="MPQSRVGLQEQGLVNLGRMYWNLCPAVLVELALARREGILASTG
                     ALAVKTGARTGRSPKDKFIVEDPASRDQIHWGRVNRPFPREQFEALYTQVLGYLQGRD
                     VFVLDAMAGADPRYQVPIRVVNELAWHNLFARQLFRRISVEEQHAHRPEYTVIAAPGF
                     QADPSRHGTHSEAFVILDLERKLILIGGTRYAGEMKKSVFSALNYELPRRGVFPMHCA
                     ANVGPEGDVALFFGLSGTGKTSLSADPKRRLIGDDEHGWSDDGVFNFEGGCYAKCFRL
                     SPKYEPQIWNALCFGAVLENVILDPETRVPDYDDDSVTENTRAAYPVEFIENAMVTGV
                     AGHPTTVLFLAADAFGILPPIARLNRNQALYHFLSGYTAKLAGTETGMGDEPEATFST
                     CFGAPFLPLAPGVYADMLGQRLDRHGSRVYLVNTGWVGGPYGVGSRISIAYTRAMVDA
                     AISGRLEGVAWRTDPVFGIEVPTECPGVPTEILDQRSTWASPSEYDHRARELAERFIR
                     NFAQFKDVSKEISQAGPRLG"
BASE COUNT          940 a         1788 c         1762 g          946 t
ORIGIN      
        1 gcgtctcttc cgacgagtag accgaaggca tgttgacccc gacgacgcgc tccttgccta
       61 gggcatcgac ggcgatgcac gcgaccatcg ccgagtcgat tccgccgctg agcccgatca
      121 ccacatcctt gaagccgttc ttggtcacgt agtcgcgtgt gccggtcacc agcgcggtgt
      181 acacctcttc caggcgatcc cgcacgggag ccgattccgc cgccaccggt gggcggtccg
      241 gcctcagtgc aaccggctcc aggatcaccc gctccaccgc ctcgcctccg gcctggcgcg
      301 cccgccgacg ctgcccccag gagggatccc gcaggcgcgc gcgggtcacg ctcgagaggt
      361 caagatcggc gaccaccagc gtctcggcaa agacgggcgc gcgcaggagc atctcgccat
      421 ccggcccgag gagcaccgag ccgccatcaa agacgagctc gtcctggccg cccgtgccgt
      481 tgagatacgc gaggaagcag gcatcctcgg cggcccggat agcaagcctc cgctcgcgag
      541 cgggccgctg gcccatcacg aagggcgcgg cgctgaggtt gaaaaccacc tgcgcccctc
      601 ccaggaggca ctgggtgctg gccggcccat ctggagccca gccctcctcg ccgatggtaa
      661 cgccgaaagt gacgccaccc agcgagaaga cgcgggtttc ctcgcccggc tggaagtagc
      721 gcgtctcgtc aaacgcaccc gacctggaga gaacgcgctt gtggtatacc ccggcgagat
      781 ggccattatg gaggacggcg gcagcgttga aaaggctctc ctggaagtcg acgaagccga
      841 cgatggccgt caggcccgtg gtcgccgcgg ccacgcgctc gagcgcgcgc cggttttcct
      901 ggagaaagcg gggtctcagc aacaagtcgt cgggcgggca cccagtgatc gcgagctctg
      961 gaaaggcaac cacgtccacc cctcgggcgc gggcctcctc cagggccgtc aggatcgtct
     1021 ggacgttccg gtccagatcg cccacccagg cgttcatctg atacaggcca attcgtagcg
     1081 tttgcattct gtatgtcctc ctggcggggc attcaccgcc tctttattct agcataacgc
     1141 ctgtctgctc gcaacgctca gcacgccggg cgaatgcgcg ggcgcatttc gcccggccgg
     1201 atcaagtcgg cgcaggaggg gtcgggcgca gcgcggaagc aaccaccgac ggccggcgtc
     1261 cagcccggcc ctggagggtg aacggatgtc acaatctcgt gatacgcgcg cggatgcacc
     1321 agccattgct ctctctggct ctccgaaggc agttggcacg ctctggggcg agacaaacag
     1381 gagcaccatc cacaatgatc tggagtcgct cttcctggcg ccggcgaggg agcgcggcct
     1441 gacgactgac gtgcttatcg cccggtcgcg gcgtttcgtt gagatcgccg aacaattggc
     1501 gccccattgg atcgaggaag cgcacagcat cgcccgcgcc gccggagtcg agggcgagct
     1561 ttacctcgcg tttatcgccg gggtctaccg cgggctcttc ctccatgagg aggagagcgc
     1621 gcctgatccg gccgcgagcg aggatgaatg cacctcctac acgatcgcgc cagcggagac
     1681 ggcgggaaac gcgatcctct tccacaagac gcgcgataac gttgcccggg cgcaatcggc
     1741 tttcgtgctc cgctccacgt caccggggat tcaggcgttc ctcgcggtga gcgacgcgag
     1801 cgttctcgcc gcgatgatga tggtgaacga gcgcgggctc gccgggtcgg ccgatatgat
     1861 ggggcttgat gaggtccggc cgcgcttccg agggatgatg aacaccttca tcctccgcca
     1921 catcgcggag tgtgcgtcgt gctgcgcgga agcgctggag atcatccgcg agtttgtcag
     1981 ccgcgggtgg tacgcgggcg gggccaagac gggcacccac tggcttttcg tggatcggga
     2041 gggcacggcc ctggaggtga gcaacaacgc cgtcgaagtg cagtctcgtc tccaccccga
     2101 gggcgtctat ttcagcctgc gcgccgacac tcccggcgcg gaggcgctgc gccaggctcc
     2161 gcgtcccgtg gagttcacca cgttccacaa cgtctcgcgc cacccgtcgc tctgccttga
     2221 gacctctatc tccgggatga cggttgagat cgaccggacc tgcccggaga agctcacaac
     2281 cgcgtgggtc acctttccgg cgcgctctct cgccttcccg ctcttcatgg ggatccgcga
     2341 gacgccgcac gcgctcatcg atgggagcgt ggatcgggcc gggcaggccg ttacgggaga
     2401 gatcaagcgg tatgaagaga tggagcaagc gatttatgcc cgccagcagg cgattcgaga
     2461 ggaagcacgc gcgctcctga cgagcgggca cgctgcgaaa gtggaagagt tgctcgacac
     2521 gtgggttcag aggcaggcga tggagaatct ggcggcgctc caggaactgg cacgcgcata
     2581 ggccagcgcc cactgtggga agaggtgttt agcccgggcg ggccctcccc cgagcgagga
     2641 gggtccgcat gactgagtta gagggccgcg aggcagccac tccatctatc gccgggacta
     2701 cttgttcttg tagtagtcag gcacggggca accgagcttc ttccactgat cgatcacggc
     2761 atccgccttg ccggcgacgc caagcacgtg cagcttgtgc ttcaccatct ccttgatcgc
     2821 ctcgcggccg gggcccaggt acttgcgcgg gtcaaactcg gccggcttct cggcgaaggt
     2881 cttgcggatc ttggcggtga gagccaggcg caggtcggta tcgatgttga ccttgcacac
     2941 gcccatctgc gagacctgag tgacggcgct ctcgggaacg cccatggtgt tgggcatgtt
     3001 gccgccgtac ttgttgatct cctcgacgaa ctcctggaga acgctgctgc tgccgtgcat
     3061 gacgagcggc aggccggggg ccttctccat gatctcggag acgcgatcga tggcgagctt
     3121 cggcgcgctc ttgaacttgt aggcgccgtg gctggtgccg atggcaacgg cgagcgaatc
     3181 gcacccggtg cgctgccaga actcggccac ctggttcggg tcggtgaggt gcttcatcgg
     3241 gtcatcgacg ccgaccacgt gctcctcgat gccgcccagc tggcccagct ccgcctcgac
     3301 ggtgacgccg cgcgggtggc agtaatcgac gacttccttg gtgatccgga tgttctcctc
     3361 gaaggggtgc ttcgaggcgt cgatcatgat gctggtgaag ccgtcatcca cgcactgctt
     3421 gcacagctcg acgctgtcgc cgtgatcgag atggatggcg atcggaaggt cgcccgaatc
     3481 ctcgacggcg gcgttgatca gggcgcgcag gtacaccatc ttcgcgtact cgcgggcacc
     3541 cttggagatc tggaggatga ggggggcgcg ctcttccttg gcggcctcga tgatcccctg
     3601 gaggatctcc atgttgttga cgttaaaggc gccgatcgcg aacccgccgt cataagcgag
     3661 atcgaacatc ggcttggtgg tcatcaacgg catagcaaag ggctcctctc tgagatagtg
     3721 gtggcaatca ggccattatg cgccagacgc gacgaaacgc cgcgcagcag gcgcgcggcg
     3781 tccgtgctcc tgcactattc acttttcggt ttcgccctag cctaggcgag gtcctgcctg
     3841 agagatctcc ttcgaaacat ctttgaactg cgcgaagttg cgtataaacc gctctgccag
     3901 ctcacgggcg cgatggtcat actcgctcgg gctcgcccac gtagagcgct ggtcgaggat
     3961 ctcggtgggc acgcccgggc attcagtcgg cacctcgatg ccaaagaccg ggtcggtgcg
     4021 ccaggcaacg ccctccaggc ggcccgagat cgccgcatca accatggccc gggtgtaggc
     4081 gatgctgatc cggctcccca ccccatacgg ccctcccacc catccggtgt tcaccagata
     4141 gacgcgcgag ccgtggcgat ccagacgctg cccaagcata tcggcataca cgccgggcgc
     4201 cagaggcagg aagggcgccc caaagcaggt gctaaaggtg gcctccggct catcgcccat
     4261 ccctgtctcg gtgccggcca gcttggccgt gtagccggag aggaagtggt agagcgcctg
     4321 attccggttc agccgggcga tggggggcag gattccaaac gcatctgcgg caaggaagag
     4381 cacggtcgtt gggtggcccg caacgcctgt gaccatcgcg ttctcaatga actccaccgg
     4441 gtaggccgcg cgcgtgttct ccgtgaccga atcatcatca tagtccggca cgcgcgtctc
     4501 cgggtcaagg atcacgtttt ccagcacggc gccaaagcaa agcgcgttcc agatctgcgg
     4561 ctcgtacttg ggagagaggc ggaagcactt ggcgtagcac ccaccctcga agttgaatac
     4621 gccatcgtcg ctccacccgt gctcgtcatc gccgatgagg cgccgttttg ggtctgccga
     4681 aaggctcgtt ttccccgtgc ccgagagccc gaagaagagc gcgacatcgc cctcggggcc
     4741 cacgttggca gcgcagtgca tcggaaagac cccacggcgc ggcagctcgt agttgagcgc
     4801 gctgaagacc gatttcttca tctcgccggc gtagcgggtg ccgccgatca ggatcagctt
     4861 gcgctccaaa tcgaggatga caaacgcctc cgagtgggtg ccatgccgtg aaggatccgc
     4921 ctgaaaccca ggagcggcga tcacggtgta ttccgggcgg tgtgcgtgct gctcctccac
     4981 gctgatccgg cggaagagct ggcgcgcgaa gagattatgc cacgccaact cgttgaccac
     5041 gcggattggc acctggtagc gcggatcggc ccctgccatc gcgtccagca cgaagacatc
     5101 tcggccctgc agataaccca ggacctgcgt atagagcgcc tcgaactgct cgcgcgggaa
     5161 gggccggttc actcggcccc agtgaatctg gtcgcggctg gccggatcct cgacgataaa
     5221 cttgtccttg ggcgaccgtc cggttcgcgc cccggtcttc accgccagcg cgccggtgct
     5281 cgccaggatt ccctcacgcc gcgccagtgc cagctccacc agcaccgcgg ggcagagatt
     5341 ccagtacatc cgtcccaggt tgaccaggcc ctgttcttgc agccccaccc tactctgcgg
     5401 cattcggcgt gctcctgttc ccgagggctc cggtcg
//
LOCUS       DAOSCV010000185         5432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9499_length_5432_cov_3.466989, whole genome shotgun sequence.
ACCESSION   DAOSCV010000185 DAOSCV010000000
VERSION     DAOSCV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9499_length_5432_cov_3.466989"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1600)
                     /locus_tag="PLY56_05975"
     CDS             complement(191..1600)
                     /locus_tag="PLY56_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOJ21062.1"
                     /translation="MENSTLGVIIGNRGFFPDHLCEEGRKEILSVLSQEGFRVVALSP
                     EETPFGSVESREDAKRCADLFKRHASEIDGVLVTLPNFGDERGVAETLRLAGLNVPVL
                     VHAFPDVPGKMTLSDRRDSFCGKLSVCNNLRQFGIRFSLTRQHTMDPQSPAFREDLRW
                     FDQVCRVVKGLRSARVGAIGSRPAAFNTVRYSEKILEAHGISVEVVDLSEILGRIGRL
                     YDTDGRVHDRIALIREYQPMMNVPEASLLKMAKLAVVLDEWIEETGVVATAMQCWTSI
                     EEFYGVAPCTVMSMMSNRLLPSACEVDITGAIGMYALTLATGVPAALLDWNNNYGEDP
                     DKCVLFHCSNVPKACFSHMQMGCQDIVGSTVGFENAHGTCVGRIKPGPFTFARVSTDD
                     LSGRIRAYAGEGEFTEDPLETFGGAGVAHIPDLQRLLHSLCANGFEHHVAIAHARVAR
                     ALGEAMGNYLGWDIQLHNA"
     gene            complement(1639..>1964)
                     /locus_tag="PLY56_05980"
     CDS             complement(1639..>1964)
                     /locus_tag="PLY56_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ21063.1"
                     /translation="LLMQIYADITGRTMEISRATQACALGAALFGAVVAGPEAGGYAT
                     AGEAQAAMTGTKERIYRPDPERHAVYQELYALYRRLHDSFGVAGTQECLADVMKKLLE
                     LKSRA"
     assembly_gap    1965..1974
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1975..3343)
                     /locus_tag="PLY56_05985"
     CDS             complement(<1975..3343)
                     /locus_tag="PLY56_05985"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HOJ21064.1"
                     /translation="MATERYSVGLDYGTNSVRAIIVEVSTGREIATAVYDYQRGDQGV
                     ITDDSNPHIARQHPADYVEGLRACTTAALEQARAEPGFAPERVIGIGVDTTGSTPLPV
                     DRDGVPLAFHAEFQENLNALAWLWKDHSAIAEAEQITAMAARHRPHYLAKSGGTYSSE
                     WFFSKIWHCLNVDPRVFAAAHSWVELCDYVPALLTGATRPEQIKRSVCAAGHKAMYSS
                     EWGGLPDAEFLAMLDPKLAGLRPRLYEQAFSSECRAGVLCAKWAQSLGLPEGIPVAVG
                     AIDAHYGAVGSGVAPGTLVKIIGTSACDITVAPQGAEALDIPGVCGIVHGSVLPGYYG
                     IEAGQSAVGDIFNWFVREICEGGASLHEKLAAEASSLRPGQSGLLALDWNNGNRSVLV
                     DARLTGLLVGQTLATTRAEIYRALIEATAFGALTIQNRLAEYGVPIERVVDHDEWMSR
                     VFGGGH"
     gene            3826..4863
                     /locus_tag="PLY56_05990"
     CDS             3826..4863
                     /locus_tag="PLY56_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ21065.1"
                     /translation="MATQAEIARRAGVSQMTVSRALRGHPHVDAATRARVLEMAREIG
                     YGAALNGDARRLASRRRGGVVRTHIVCILRYAPGQARVWRDPWAGLLSEAMQAPLFDA
                     EMQVLLADIRESRVPANLRAGVVDGILAICPRSDVVEALTKLDVPVVGVANTLLPSYA
                     CQDDYAVGRIATEHLLSLGHTRIALFHPLASPNARLRQQGFVDALESAGRSPDPDLIV
                     TAEGERAEEAVPLFQELWRRRQDFTALVAYNDEVAAWLLRAAEGQGIEVPRDLSIIGV
                     DDLPWVTLLKPSLTTVRLPLMEIGEAAARILLQRLTAWWRGEPDPPAGEAACRLPVEL
                     VVRGTTAPAPR"
     gene            4877..>5432
                     /locus_tag="PLY56_05995"
     CDS             4877..>5432
                     /locus_tag="PLY56_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HOJ21066.1"
                     /translation="MAKLKAIPQSEFEQRWRTLQEKMAAHDLDVVIAHGDEADPANVR
                     YLADYWPLFESGGVAIGRTGDPILLIGPETLTYAQTRGKTPAIKQILQYRESAEPDYP
                     GMKLNTFEEVFGELAGGKPKRIGIAGLSVMPVTVYQAIKEAAGNAEVVRADDLLTEMR
                     MVKSENEIALMREAFRISELATEKV"
BASE COUNT          909 a         1806 c         1786 g          921 t
ORIGIN      
        1 tcggttgtca cgcggcgcac caggccgccg cttgcttcca aagcgttttc gtaagaaggc
       61 atgcgattac tcccaatcgg gccacttctc cgtggcctgt ttcatggcga cccaagagac
      121 tgggcgccaa agcgtcgcaa ccgcgctccc gccggagcgc gtctcttccc atgctgccag
      181 atcatgcggt ctaggcgttg tgcaactgaa tatcccaccc gaggtagttg cccatcgcct
      241 cgccgagggc gcgcgccacg cgcgcgtgtg ctatcgcgac gtggtgctcg aatccgttcg
      301 cgcacagcga gtgcagcagc cgctggagat ccgggatgtg ggcgacgccg gcgccgccaa
      361 acgtctccag gggatcctct gtgaactcgc cttcgcctgc gtaggcgcgg atgcggcccg
      421 acagatcatc ggtggagacg cgcgcgaagg tgaacgggcc cggcttgatg cggcccacgc
      481 aggtgccgtg ggcgttctcg aagccgaccg tgctcccgac gatatcctgg cagcccatct
      541 gcatgtgcga gaagcatgcc ttcggcacgt tgctgcagtg gaagagaacg cacttgtccg
      601 ggtcctcgcc gtagttgttg ttccaatcga gaagcgccgc gggcacgccc gttgccaggg
      661 tgagcgcgta cattccgatc gcgccggtga tgtccacctc gcaggcgctc gggagaaggc
      721 ggttgctcat catgctcatc accgtgcatg gcgccacccc gtagaactcc tcaatggagg
      781 tccagcactg catcgccgtg gccactacgc cggtctcctc aatccactcg tcgaggacga
      841 ccgccagctt cgccatcttc aagagcgatg cctctggaac attcatcatc ggctggtact
      901 cgcgaatgag cgcgatcctg tcgtgcaccc ggccatcggt gtcatagagg cgcccgatgc
      961 gcccgagaat ctccgataga tccaccactt ccacggagat gccatgggcc tcgaggatct
     1021 tctcgctgta gcgaaccgtg ttgaaagcgg ctgggcgcga cccgatcgcg cccacgcggg
     1081 cactgcgcag ccctttcacc acacggcaca cctggtcgaa ccaccggaga tcctcgcgga
     1141 acgccggcga ttgcgggtcc atcgtatgtt gccgcgtcaa cgagaagcgg atgccaaact
     1201 ggcggaggtt gttgcacacc gagagcttgc cgcagaagct atcgcgccgg tcagagagcg
     1261 tcatcttgcc aggcacatcg gggaaggcgt ggacgagtac cggcacgttg aggccggcca
     1321 ggcggagggt ctcggcgacg ccgcgctcat ctccaaagtt cggcagcgtc acgagcacgc
     1381 cgtcgatctc gctcgcgtgc cgcttgaaga ggtcggcgca ccgcttcgca tcctcgcggc
     1441 tttccaccga gccaaagggc gtttcctccg ggctcagggc caccacgcgg aaaccttcct
     1501 gcgagagcac cgaaaggatc tccttgcgcc cctcctcgca caggtgatcg gggaagaagc
     1561 cccggttgcc gatgatcacg ccgagtgtgg agttttccat cagttccgcc tcctcaagcc
     1621 tccgtggacg ggggcgttct acgcccggct cttcagctcc agcagcttct tcatcacatc
     1681 cgccaggcac tcctgcgtgc cggcgacgcc gaagctatcg tgcaggcgcc tatacagcgc
     1741 gtacagctcc tggtaaaccg cgtgccgttc cggatcgggc cggtagatcc tctccttggt
     1801 gccggtcatc gccgcctggg cttctccggc ggtcgcgtag cctcccgcct cggggcccgc
     1861 gaccacggcg ccaaagagcg ccgcgcccag ggcgcacgcc tgcgtcgcgc gcgagatctc
     1921 catcgtgcgg ccggtgatat cggcgtaaat ctgcatcagg agggnnnnnn nnnncgtggc
     1981 cgccgccgaa caccctgctc atccactcgt cgtggtcgac aacacgctcg atcggcacgc
     2041 catattcagc caggcggttc tgaatggtga gcgcgccgaa cgccgtcgcc tcgatcagcg
     2101 cccggtagat ctcggcgcga gtggttgcca gcgtctggcc cacgaggagg ccggtgagcc
     2161 gggcgtcgac gagaaccgac cggttgccgt tgttccaatc gagggcgagg aggccgctct
     2221 ggcccgggcg gagcgaggac gcctctgcgg ccagcttctc atggagactg gcgccgcctt
     2281 cgcaaatctc gcgaacgaac cagttgaaga tgtcgcccac ggccgactgg cccgcctcga
     2341 tgccgtaata cccggggaga accgagccat gcacgatgcc gcacaccccc gggatgtcga
     2401 gcgcctccgc cccctggggc gccacggtga tgtcgcaggc ggaggtgccg atgatcttca
     2461 cgagcgtgcc aggcgccacc ccggagccaa cggcgccgta gtgcgcgtca attgcgccca
     2521 cggcgaccgg gatcccttcc ggaagaccga gtgactgcgc ccacttggcg cagaggacgc
     2581 cggcgcggca ctcggaggaa aatgcctgct cgtagagccg cgggcgcagc ccggccagct
     2641 tcggatcgag catcgccagg aactccgcgt ccggcaggcc gccccactcg ctggagtaca
     2701 tcgccttatg cccggcggcg caaacgctcc gtttgatctg ctctgggcgc gtcgcccctg
     2761 tgaggagcgc tggcacgtag tcacacagct ccacccagct atgcgccgcg gcaaagacgc
     2821 gcgggtccac gttcagacag tgccagatct tcgagaagaa ccactcggat gagtaggtgc
     2881 cgcccgattt ggcaaggtag tgagggcggt gccgggccgc catcgccgtg atctgctccg
     2941 cctcagctat ggcggagtga tccttccaaa gccacgccag ggcgttcagg ttctcctgga
     3001 actctgcgtg gaaggcgagc ggcacgccat cgcggtcgac cggcaagggg gtgcttccgg
     3061 tggtatccac gccgatcccg atcactctct ccggggcgaa gccgggctcg gcgcgcgcct
     3121 gctcgagcgc ggcggtcgtg cacgcgcgga ggccctccac gtaatcggcc ggatgctgcc
     3181 gggcgatgtg cgggttggaa tcgtcggtga tcacgccctg gtcgccgcgc tggtagtcgt
     3241 acaccgcagt ggcgatctcg cgcccggtac tcacctcgac gatgatggca cgcacggagt
     3301 tggtgccata gtccagtccg acggagtatc tctcggtagc caatgttgcc tccttcgccg
     3361 ccgtgaggat agagagaagc gctgagaagg gccggccagc ccgccgggca cctgtgccat
     3421 gttcaggaga agtctaacat ggttgcaggt cggtgtcaat caacacatcg ggtgaagctg
     3481 cgccgccatc gataccacgg atgaacggag aggctccatt ccggtgcgcg gagccaaagc
     3541 gccgcgctga ttccagacaa agcccttcgg gctcccccag gcctcgccgt cgccggctac
     3601 ctgggagtgc aggccggcct agcttggcag ggcttcgctc gcaagcagcg cggtgatcta
     3661 tcgccgcgcg agacatggag ggccggttca tctgcgttca tctacggata accacggacc
     3721 gggcgcctgc ccagcaggcg cctggcctcc gttgacaggc ctgtctgctt gtgttacact
     3781 aggccggtcg cgctgtttac gcaaacatcg cgggggggct ttcacatggc gacgcaagcg
     3841 gaaatagcca ggcgggcggg tgtcagccag atgacggtct ctcgcgcgct ccgcggccac
     3901 ccccacgtgg atgcggcgac gcgagcgcga gtgctcgaga tggcgcgcga gatcggctac
     3961 ggcgccgcgc tgaatggcga cgcgcgccgt ctggccagcc ggcgccgcgg tggcgtggtg
     4021 cgcacccaca tcgtctgcat cctccgctac gcgccgggac aagcccgcgt gtggcgcgat
     4081 ccttgggccg ggctcctttc ggaggcgatg caggccccgc tcttcgacgc ggagatgcag
     4141 gtactcctgg ccgatatccg cgaaagccgg gttccggcga atctccgcgc gggcgtggtg
     4201 gatggcatcc ttgcgatctg tccccggtct gatgtggtcg aggcgctcac gaagctggat
     4261 gtgccggtcg tgggggtagc caacacgctc cttccctctt acgcctgcca ggacgattac
     4321 gccgtcggtc ggatcgccac ggagcacttg ctctccttgg ggcacacgcg gatcgcgctc
     4381 ttccatcccc tcgcatcgcc gaatgcgcgg ctgagacagc aaggctttgt ggatgccctg
     4441 gagagcgccg ggcgctctcc cgatccggac ttgatcgtca ccgccgaggg cgagcgtgcg
     4501 gaagaggcgg tgcctctttt ccaggaactt tggcggcgca ggcaggactt taccgccctg
     4561 gtcgcctata acgatgaagt agccgcgtgg ctcctacgcg cggcagaggg ccaggggatt
     4621 gaggttcccc gtgacctgag tatcatcggc gtggatgacc tcccctgggt caccctgctg
     4681 aagccgtcac tcaccacggt gcggcttccc ttgatggaga tcggtgaggc cgccgcgcgc
     4741 atcctcctcc agcgcctgac agcctggtgg cgcggtgagc ctgatccgcc ggcgggtgaa
     4801 gctgcctgcc ggttgccggt ggagctggtg gtgcgcggca cgacagcgcc ggcaccccgg
     4861 taatggagag gcaaagatgg cgaagttgaa ggcgattcct cagtcggagt tcgagcagcg
     4921 ttggcggacc ctccaggaga agatggccgc ccatgacctg gatgttgtga ttgcccacgg
     4981 cgatgaggcg gaccccgcca acgtgcgcta cctggcggac tactggccgc tcttcgagtc
     5041 tggcggtgtg gcgattgggc gcacgggcga cccgatcctc ctcatcggcc ccgagacgct
     5101 cacctacgcc cagacgcgcg ggaaaacccc ggcgatcaag caaatcctcc aataccgcga
     5161 gtcggccgag ccggactacc cgggcatgaa gctgaacacc ttcgaggaag tcttcggcga
     5221 gctggccggc ggaaagccga agcggatcgg catcgcgggt ctctcggtga tgcccgtcac
     5281 ggtctaccag gcgatcaagg aagccgctgg taacgccgag gtcgtccgcg ccgacgacct
     5341 gctgacggag atgcgcatgg tcaagagcga gaacgaaatc gcgctcatgc gcgaggcgtt
     5401 ccggatcagc gagcttgcca ccgagaaggt gc
//
LOCUS       DAOSCV010000186         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9528_length_5421_cov_3.199031, whole genome shotgun sequence.
ACCESSION   DAOSCV010000186 DAOSCV010000000
VERSION     DAOSCV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9528_length_5421_cov_3.199031"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..2619
                     /locus_tag="PLY56_06000"
     CDS             229..2619
                     /locus_tag="PLY56_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ21067.1"
                     /translation="MGWPGFCFILLLVATQAMAAPKRGENLLANPGFEQAVETAAASW
                     NPAGKGYTLAPGSGRRGGYAIACAANQPDEYYGAMQEVHFNPPLQHPFEVSGWSKAEN
                     AEGIDYCLYMDVFYADGTNLWGVRGDFSRGTHDWERVSYTFDVKKPVAKIQYFILFRK
                     CTGKAWFDDVSISLVPFRVESERLVAGLGGGNNLEFRGILSFPGRWTASVLRGDEAVY
                     SASGEGEQIAFQWPGTDSAGRALPGGAYTLRLDAQETSSDEALRREWPVRTPRGPGAA
                     YLAWVERSMHRVLRDALPPSRKRPLRASVALAGNEYESFQLCLRATPGKDLEACTVSL
                     SDLRDSRGNVLKKEHFEWHQVGFVKISELYPHPEMKDALPGWWPDPLLPVSRFDVSGG
                     TTQSVWFTLYAPPGTPAGTYRGEFTVTPGNAPALKIPVEARVYGFTLPTQPRLKTAFA
                     LMDGFLESVYGPLTPELRRKYGDYVLRHRLNPDDISRTDPPGLDDLTHYTSRGLNAFN
                     VQNLVEPRGKRTWVCWSPISVYTPQFKESLIQRLDPYAAELKRRGLASKAYVYSFDER
                     GEEFWPIIREYFGLVKERWGIPTLTTAKIPQDPAVMKELNVDWNCPLTARYDLDAAER
                     CRAAGLQVWAYVCCSPRYPYANWLADDPLVEARVIWWQAYHQKMDGMLYWGLNIWGRA
                     NNKTMIDPEKDGPLLNFGITTEAVWADRLHGDGLLLYPGKDGPIGSIRLANIRDGLED
                     YDYLSLLAEREGSIEKARQACLPVTTSLTQFTRDPETVLAQRDRIARRIERAGAKR"
     gene            2674..4005
                     /locus_tag="PLY56_06005"
     CDS             2674..4005
                     /locus_tag="PLY56_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21068.1"
                     /translation="MAEVENQPALEAGAAQVCITPPVGTQLAGYFHQRVSESVRDDLF
                     ARALVLRQGNAAVALVSCDLICIEKGFAQPAKERIAARTGIPPENVLLCATHTHTGPE
                     VRPGRICEVNEPWLERLPGLIADAVVTADQSRFAAQLSPARGSEGDLSFNRLFRMKDA
                     TELFGRGPAGSAIGPAGGIDPEVLVLKVSDSEGRPRAFVVNHALHVDVIGGGGGRFLS
                     ADWPGEMARALSAIYGQDVVTLFLNGCCGDINHVPHQPTSLPRSGPAKSVQLGRAFAG
                     VAMNAAEKAEPVAVDRIAARLEELAIPYYTRESAMRAYVDSLREKPNRSDFEEYTIKA
                     FDEWPLDGQMATVPVQAFRVGEIAFVGLPGEIFVAHGLEVKKWSPAAFTFIAELANDW
                     FGYVPTTDQAERGAYGARPILSRRLSSDAGRRMTDAIQKALWKLWEEDAGQ"
     gene            complement(4060..5313)
                     /locus_tag="PLY56_06010"
     CDS             complement(4060..5313)
                     /locus_tag="PLY56_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21069.1"
                     /translation="MAEKKVRVGVFGIGRGRMFARQAAAMEGVELVAICDQREKPMRE
                     FAAQYPGVATYTEYERFLEHDMDAVVLANYATEHAPAAIQAMRAGKHVQSENIAVKTM
                     AEAVALVRAVEETGKIYMYAENCCYMNTVQEIRRLYAAGELGEFRYGEGEYVHPVSPD
                     EKIRLSPTWDHWRNWLPATYYCTHAMGPLMYISRTRPVQVTGFEVPYDFEDPTCTQTA
                     KRSDTAGIIICRMDNGGYVKLLQGNLKCHLNWYRIYGNRGSAETLRHGDTASIRVRKE
                     PWEKGEEEPVERIYRPEFPEFHDLASRTGHGGGDFFVVHEFIRAIRTGEPPYLDVYTG
                     LQMSCVGILAHRSALNNNATYEVPRFRDPAELKRWENDHLSPDPADPTEARIPASTLG
                     EIEKPPEVRASFERRARELCPDWYE"
BASE COUNT          881 a         1840 c         1789 g          911 t
ORIGIN      
        1 cagtgggtgc ctttctccta ccgcttcacc acgcccccgg aaaccgccgc gatccggctc
       61 tggatccata gcttcaacgc ttcgcaggtc gaggcgttcc tggacgacct ggaagtgcgc
      121 ctagtgaagt agtacgcggc atgccccttc cccctcttgg cccggtggga aggggctcgc
      181 ctatccatgt gccggccgcc tggccggcgc cacggaggag aagttattat gggatggcca
      241 ggcttctgct ttatcttgct gctggtggcg acgcaggcga tggcggcgcc aaagcgcggt
      301 gagaacctgc tcgccaatcc gggatttgag caggcggtcg agaccgcagc cgccagctgg
      361 aaccctgcgg gcaagggcta cacactcgcg cccggctccg ggcgaagagg cggctacgcg
      421 atcgcctgtg cggccaacca gccggacgaa tactacggcg cgatgcagga ggtccacttc
      481 aacccgccgc tccagcaccc gttcgaggtc tcgggctgga gcaaagcgga gaacgcggag
      541 ggcatcgact actgcctcta catggacgtc ttctacgccg atggcacgaa cctatgggga
      601 gtgcgcggcg acttctctcg gggcacgcac gactgggagc gcgtctccta caccttcgac
      661 gtgaagaagc cggtcgcgaa gatccagtac ttcatcctct tccgcaagtg taccggcaag
      721 gcgtggtttg acgacgtttc gatctcgctc gtcccgttcc gggtggagtc cgagcgcctg
      781 gtggccggcc tgggcggggg gaacaacctg gagttccgcg ggatcctcag cttcccgggg
      841 cggtggacgg catccgtcct gcggggagac gaggcggttt actccgcatc gggcgagggg
      901 gagcaaatcg ccttccagtg gcccggcacc gattccgccg ggcgcgcgct ccccggcggc
      961 gcctacacgc tccgcctgga tgcccaggaa accagctcgg acgaggcgct gcgccgcgag
     1021 tggccggtgc gaaccccgcg cggcccggga gcggcctacc tggcctgggt ggaaaggagc
     1081 atgcaccgtg tgctgcgcga cgcgctgccc ccctcccgga agaggccgct ccgcgcctcc
     1141 gtagcgctcg cgggcaacga gtacgagagc ttccagctct gcctgcgcgc caccccgggc
     1201 aaggacctgg aggcatgcac cgtctcgctg agtgacctgc gcgacagccg cgggaatgtg
     1261 ctgaaaaaag agcatttcga gtggcaccag gtcggcttcg tgaagatctc agaactctac
     1321 ccgcatccgg agatgaagga cgctctgccg ggctggtggc ccgatcccct gctccccgtg
     1381 agccgtttcg acgtgtcggg cggcacaacc cagagcgtgt ggttcaccct ctacgccccg
     1441 ccgggcaccc cagccggcac ctaccgcggc gagttcaccg tgacgcccgg aaacgcgccg
     1501 gcgctaaaga tcccggtcga agcccgcgtt tacggcttca cgctgcccac ccagccccgg
     1561 ttgaagacgg cgttcgcgct catggatggc ttcctggaga gcgtttacgg gccgctgacc
     1621 ccggaactgc ggcgcaagta cggcgactac gtcctccggc accggctcaa tccggacgac
     1681 atctcgcgca ccgacccgcc gggcctggac gacctgaccc actacacctc ccggggtctg
     1741 aacgccttca acgtgcagaa cctggtggag ccgcgcggga aacgcacctg ggtctgctgg
     1801 agcccgatta gcgtctacac gccacagttc aaggagagcc tgatccagcg cctcgaccct
     1861 tacgcggcgg agctgaagcg gcgcgggctg gcgagcaagg cctacgtcta ctccttcgac
     1921 gagcgcggcg aggagttctg gccgatcatc cgcgagtact ttggcctggt gaaggagcgc
     1981 tgggggattc ccaccctgac cacggccaag atcccgcaag acccggcggt gatgaaggag
     2041 ctcaacgtcg attggaactg cccgctcacg gcgcgctacg atctggacgc cgccgagcgc
     2101 tgccgcgcag ccgggttgca ggtgtgggcc tacgtctgtt gctcgccccg ctatccctat
     2161 gccaactggc tggccgacga cccgctcgtc gaggcgcgcg tgatctggtg gcaggcgtat
     2221 caccagaaga tggacggcat gctctattgg ggcctgaaca tctggggccg cgcaaacaac
     2281 aagacgatga tcgacccgga gaaggacggc cccctgctca actttggcat caccaccgaa
     2341 gcggtatggg ccgaccggct tcacggcgat ggcctgcttc tctacccggg gaaggatggc
     2401 ccaatcggca gcatccggct ggcgaacatc cgcgacggcc tggaagatta cgactacctc
     2461 tcgctgctcg cggagcgcga gggctccatt gagaaagcgc gccaggcgtg cctgccggtg
     2521 acgaccagcc tcacccagtt tacgcgcgat cccgaaacgg tcctggccca gcgcgaccgg
     2581 atcgctcgcc gaatcgagcg cgccggagcg aagcggtagt cgtctgccct tcgcggaagc
     2641 tcccacgaga gcagggattg gatggaggag agtatggctg aagttgaaaa tcaaccggcc
     2701 ctggaggcag gggccgctca ggtctgcatc accccgccgg tgggcacgca gctggccggg
     2761 tatttccacc agcgcgtctc cgaaagcgtg cgcgacgacc ttttcgcgcg cgcgctggtg
     2821 ctccgccagg ggaatgcggc ggtagcgctg gtctcgtgcg acctgatctg catcgagaag
     2881 gggttcgcgc agcccgccaa ggagcggatc gcggcgcgaa ccgggatccc tcccgagaac
     2941 gtgctcctct gcgccacgca cacgcacacc ggcccggagg tgcgccccgg ccggatctgc
     3001 gaggtgaatg agccgtggct ggagcgcctg cccgggctga ttgccgatgc cgtggtcacc
     3061 gccgaccaat cgcgtttcgc ggcgcagctc tctcccgcga ggggttcgga gggcgatctc
     3121 tcgttcaacc gcctcttccg catgaaggac gctaccgaac tgttcgggcg cggccctgcc
     3181 ggaagcgcca tcggccccgc tggcggcatc gaccccgaag tgctcgtgct gaaggtgagt
     3241 gactccgagg gccgaccgcg cgcgttcgtg gtcaaccacg ccttgcatgt ggacgtcatc
     3301 gggggtggtg gcggacgctt cctctccgcg gactggccag gcgagatggc gcgcgctctc
     3361 tccgcgatct acggccagga cgtggtcacg ctcttcctca acggctgctg tggcgatatc
     3421 aaccacgtgc ctcaccagcc gacttcgctg ccccgcagcg ggccggcgaa gtcggtgcag
     3481 ctgggtcggg cgttcgccgg cgtcgccatg aacgccgccg agaaggccga gccggtggcg
     3541 gtcgaccgga tcgcggctcg cctggaagag ctcgcgatcc cctactatac gcgcgagagc
     3601 gcgatgcgcg cgtatgttga tagcctgcgc gagaaaccga accggagcga tttcgaagag
     3661 tatacgatca aggcgttcga cgagtggcct ctggacgggc agatggcgac ggtgccggtg
     3721 caggcgttcc gcgtcggcga gatcgccttc gtcggcctac cgggcgagat cttcgtcgcc
     3781 catggtctgg aggtgaagaa gtggtcgccc gccgccttca ccttcatcgc ggagctggcc
     3841 aacgactggt tcggctacgt accgacgacc gaccaggcgg agcgcggagc ctacggcgcg
     3901 cggcccatcc tctcgcgccg gctctcttcc gatgccggcc ggcggatgac ggacgcaatc
     3961 cagaaggcgc tgtggaagct gtgggaggag gacgccggcc agtaacgccc ggtatcggcc
     4021 gagcaggcgg cgcttcgcgc cgcctgctcg gctcaggtct cactcgtacc agtcggggca
     4081 cagctctctc gcgcgccgct caaacgaggc gcgtacctcg ggcggcttct caatctcgcc
     4141 cagggtgctc gcggggattc gcgcctcggt cgggtctgcc gggtcgggcg agagatggtc
     4201 gttctcccaa cgcttcagct ccgcggggtc gcggaaacgc ggcacctcgt aggtggcgtt
     4261 gttgttgagc gcgctccggt gcgccagtat ccccacgcag ctcatctgca gcccggtgta
     4321 cacgtccagg tacggcggct cgcccgtgcg aattgcgcgg atgaactcgt gaacgacaaa
     4381 gaaatcgcca ccgccatgcc cggtgcgcga ggccagatcg tgaaactccg ggaactccgg
     4441 cctgtagatc cgctcgaccg gctcctcctc gcccttttcc cagggctcct tacgcacgcg
     4501 gatcgaggcg gtgtcgccgt ggcggagcgt ctcggcgctc ccccggttcc catagatccg
     4561 gtaccagttg aggtggcact tcaggttccc ctggagtagc ttgacatagc cgccgttatc
     4621 catccggcag atgatgatgc ccgccgtgtc gctccgcttg gccgtctggg tgcaagtggg
     4681 gtcctcgaag tcgtagggca cctcgaagcc ggtcacctgc accgggcgcg tccgcgagat
     4741 gtacatgagg gggcccatgg cgtgggtgca gtagtaggtg gcggggagcc agttgcgcca
     4801 gtggtcccag gtgggcgaga gccggatctt ctcgtccggc gagacggggt ggacgtactc
     4861 cccctccccg tagcggaact cgcccagctc gcccgcggca tagagccggc ggatctcctg
     4921 gaccgtgttc atgtagcaac agttctcggc atacatgtag atcttgccgg tctcctccac
     4981 cgcgcggacc agggccaccg cctcggccat cgtcttcacc gcgatgttct cgctctgcac
     5041 atgcttgccc gcgcgcatcg cctggattgc cgcgggcgcg tgctccgtgg cgtagttcgc
     5101 caggaccacg gcgtccatat cgtgctccag gaagcgctcg tactccgtgt aggtcgccac
     5161 cccgggatat tgcgccgcga actcccgcat cggcttctcg cgctggtcgc agatcgcgac
     5221 aagctcgacg ccttccatcg cggccgcctg ccgggcgaac atccgccccc ggccaatgcc
     5281 gaacaccccg acgcgcactt tcttctccgc catcttttcc cccggttcct tccgaatgcc
     5341 tcccgcgcgc tcaaagtacc cctgcccgca tgattcatca gaataatgaa aagaagtctc
     5401 tctgccaagc gtggtgcggg t
//
LOCUS       DAOSCV010000187         5419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9532_length_5419_cov_3.111484, whole genome shotgun sequence.
ACCESSION   DAOSCV010000187 DAOSCV010000000
VERSION     DAOSCV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9532_length_5419_cov_3.111484"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3577
                     /locus_tag="PLY56_06015"
     CDS             <1..3577
                     /locus_tag="PLY56_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ21070.1"
                     /translation="LSDSGKLTLYRGATPVKKAAVAGKPVRIEVNAGRVRVLGTPEGG
                     EGEPVCLLEYADSKPLARGRVGVRFARWVSTETVARAARVASPRLAEYRFDTAPVDWG
                     IEAGEWIATTRWACVPHWNFFGGRGDPMATLWNKRRFGGDQWIEVFVAPREGTSDRMH
                     FTAPINLNLSFCAEKEQIGSGYSLLYRSHDEPALLYRRGKVVAENREIVLLRWRNDQM
                     FVYYRITQTWHHFVILREGGRIRVWVEVPSYGRGRLERRLLFDYTDPDPLPGDRLGLW
                     TWGDNGMAVARVRVCAARRGAPVASFLQAPASPTAAGDEQVSVNPINGGWFRTDLGRW
                     VDPGSKPVVAFEFQADPRAALALYAVAGGQRFRARLLGDVTEDGDSVPMGSVRLTKLA
                     GGWTRAEFPLRQALRTFFPSGRVPVVEQLFLANLSTRPELIAGLTANPKGCVFRWRRV
                     SPGASPRSMPAFRLATPKSEEVPAGDLRIAVADTRLDPETCAVVVNGKRLRLGTPGLR
                     WDGIRGEFVVNLTAAGFRFRDGERVGLQVMAPGQDALSLAWRMRVGADKVPPTAPVVA
                     TPSGPDRVDTFEQDVGSWRRLGGEQGGTPWRDETTKASGRFSLRLTHRRVAGSFGTAV
                     REKPFDVRRWPRLTFWYRVRPEVHLSLVLEIDGKWYEIGFTDRDSTFPVIGAIAGVKA
                     DGTWRRAEVDLAAAVRASGAPTTVISKLFFADTGWMNNIQGISWHVDDFRFVPALPVG
                     SNEALTLHADDLSGIAGYSWVFDNAAETVPDTTIEMGPARIPPGASIFHVRACDGAGN
                     WGPTTHFRFVPLEAGDGPAPEATPLVASGASIPAPAMDVTLKNAGALDVESVRWWVRR
                     GGLLKAYDLSGGGLRWDRAKGVIRWEDPTLATEKPAPLLCGLKARDLAGKTVLYHEWR
                     WTVDPGLDTMPPAAPYVSYIPQNRLSRIDFEHGVPPEVELRRSAWVFHESGDAAVGRG
                     SARVVNLEADDFFSVFLRKTPYAVHDYPRVRFDYRFETAGCNLNIVSVVNGDLQTIGF
                     AGENGAYPPFVQNNIGRIKGVKQDGQWHAAEFDFGALLRRRYPNMPRFFADYLGTWAT
                     GVRSTYDNPGGVSLWLDNITIYSPRATSAAFEWQPPADPNGIRGYSYVVDQKQETLPN
                     ERIVTREPRCHVENLKPGAWVFHVRACDGAGNWGPAAHLPFQLTQ"
     gene            complement(3650..5233)
                     /locus_tag="PLY56_06020"
     CDS             complement(3650..5233)
                     /locus_tag="PLY56_06020"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ21071.1"
                     /translation="MDPTASTEGTTEAVETRRAPRAPCCNGTPAAPQWRDTLRWLIAL
                     GSTLAALAITLAARDLVAPTVFVLFFAAVAISAWFGGLLPGLLSTALSAWLIHQYVEP
                     RDPTTPWRVDELVRLAFFMGVAILISSLSDSLRRTQTALRAIIQASPLPIVTLDWSGH
                     IRGWNPAARRAFGWQSPQMLGRLLPLADDEDPTLLAERVCTRGVIHGLETRIASGDYH
                     VIDLSLSAAPLPDASGRTTGILIIAEDITERKRVLNALRATQEETRQALGRERRIAER
                     FQAALLPDQCAAVPGYAIAHTYRPALIEADVGGDFYNIFPMDDRYLCLVIGDVGGKGL
                     DAAVIGARMQHGISALALHERGHPEIILDGIQQTFAAAGDPDRLATLFLAFLDRETGR
                     LTYASAGHEPALVWRAREQRVEQLTTHGSALIPAANGPYDSRETVLTPGDILVLYTDG
                     LVDPHRPGDPDLFGVERVAATLAAHSAEGPSTILNTLYDAAAAHSAGNLRDDIALMAI
                     RRLDDPQQPGEYVFVTPPA"
BASE COUNT          877 a         1679 c         1933 g          930 t
ORIGIN      
        1 cttgtcggat tccggaaagc tgacgctcta tcggggcgcg acgccggtga agaaggcggc
       61 ggtggcaggg aagcccgtgc gcatcgaggt gaacgccggg cgcgtgcggg tgcttggcac
      121 gcccgaaggc ggcgagggag agccggtgtg cctgctggag tacgccgact cgaagccgct
      181 tgcgcgcggg cgcgtgggcg tgcgcttcgc ccggtgggtc tcaacggaaa ccgtggcgcg
      241 cgcggcgcgc gtggcctcgc cgcggctggc agagtaccgc ttcgataccg cgccggtaga
      301 ctggggcatc gaggcgggcg agtggatcgc caccacgcgg tgggcgtgcg ttccccattg
      361 gaacttcttc ggcggtcgcg gcgatccgat ggccacccta tggaacaagc gccggtttgg
      421 tggggatcaa tggatcgaag tgttcgttgc tcctcgggag ggcacgagcg accgaatgca
      481 cttcaccgcg ccgatcaacc tgaacctgag cttctgcgcg gagaaggagc agatcggctc
      541 tggctactca ctgctctatc gctcgcatga cgagcccgcg ttgctctacc gtcggggcaa
      601 ggtagtggcc gagaaccggg agatcgtcct cctgcgatgg cgcaacgacc agatgtttgt
      661 ttactaccgg atcacgcaga catggcacca cttcgtgatc ctgcgcgaag gcgggcggat
      721 ccgagtgtgg gtggaggtgc cctcttacgg ccggggacgc ctggagcgtc ggcttctctt
      781 cgactacacc gaccccgatc cgcttcctgg agaccgcctg gggctttgga cgtggggtga
      841 taacggcatg gcggtggctc gcgtgcgcgt ctgcgcggcg cgccgcgggg cgcccgtggc
      901 cagcttcctc caggcgccgg cttctccgac cgcggcgggc gacgagcaag tcagcgtcaa
      961 tccgatcaac ggcgggtggt tccgcaccga cctggggcgt tgggtggacc ccggaagcaa
     1021 gccggtggtg gcgttcgagt ttcaggcgga tccgcgagcg gccctggcgc tctacgcggt
     1081 ggccggcggg cagcgattcc gcgcgcggct tctgggtgat gtcacggagg atggcgactc
     1141 cgtgccgatg gggagcgtgc gcctgacgaa gctcgccggc ggatggacgc gcgccgagtt
     1201 tccgctgcgc caggcgttgc gcaccttctt tccctcgggt agggtgcccg tcgtggagca
     1261 gctcttcctt gccaacctga gcacccggcc ggagctgatc gccgggctca cggccaaccc
     1321 aaaggggtgc gttttccggt ggcggcgtgt ctcgccgggc gcgtctcctc gatccatgcc
     1381 ggccttccgt ctggcaaccc ccaagagcga agaggtgccg gcgggtgacc tgcgcattgc
     1441 cgttgccgat acccggctcg acccagagac gtgtgccgtc gtggtgaatg ggaagcgcct
     1501 gcggttgggc acgccgggcc tgcgctggga cggcatccgt ggtgagttcg tcgtcaacct
     1561 caccgcggcc gggttccgat tccgcgatgg tgagcgggtg ggcctgcaag tcatggcgcc
     1621 ggggcaggac gcgctctcgc tggcctggcg tatgcgcgtg ggcgcggata aggtgccgcc
     1681 cactgcgccg gtggtagcca ccccctcggg gccggaccgc gtcgatacct tcgaacagga
     1741 cgtgggctcc tggcggcgcc tgggcggcga gcagggaggg acgccctggc gcgatgagac
     1801 tacaaaggcc tctggtcgct tcagcctccg actcactcat cgccgtgtgg ccggctcttt
     1861 cggcacggcc gtgcgcgaga agccgttcga tgtgcgccgc tggccgcggc tcaccttctg
     1921 gtaccgcgtt cgtcctgagg tgcacctcag cctggtcctg gagatcgacg gcaagtggta
     1981 cgagatcggg tttaccgacc gcgacagtac cttcccggtg atcggcgcga ttgctggggt
     2041 gaaggcggac ggcacgtggc ggcgggcgga ggtggatctg gcggcagcgg tgcgcgcctc
     2101 gggggcgccc acgaccgtca tctcaaagct gttcttcgcc gataccggct ggatgaacaa
     2161 tatccagggg atctcctggc acgtggatga cttccgcttc gtgcccgcgc tccccgtggg
     2221 atcgaatgag gcgctcacct tgcacgccga tgacctctcc ggaatcgcgg gatacagctg
     2281 ggtcttcgac aacgccgcgg agaccgtgcc ggatacgacg atcgagatgg ggccggcccg
     2341 cattccaccc ggcgccagca tcttccatgt gcgcgcctgc gatggcgccg gcaactgggg
     2401 cccgacgacc catttccggt tcgtaccact ggaggcggga gatggtccgg caccggaggc
     2461 gacgcccctc gtggcctccg gcgcgtcgat tccggcaccg gccatggatg tgacgttgaa
     2521 gaacgcgggc gctctcgacg ttgagagcgt gcgctggtgg gtgcgacgcg gcggtctgct
     2581 caaggcctac gacctttccg gcggcggcct gcgatgggat cgggcaaagg gagtcatccg
     2641 ctgggaagac ccgacgctgg caacggagaa gccggcgccg ctcctgtgtg gcttgaaggc
     2701 gcgcgacctg gccggcaaaa cggtcctcta tcacgagtgg cgctggaccg tcgatccggg
     2761 gctcgacaca atgccgcccg ccgcgcccta cgtctcgtat attccccaga accggctgtc
     2821 gcggattgac ttcgagcacg gagtgccgcc ggaagtggag ctgcgccggt cggcatgggt
     2881 gtttcatgaa tccggagacg cggccgtggg gagaggctcg gcgcgcgtgg tgaacctgga
     2941 agccgatgat ttcttctcgg tgttcttgcg gaagacgccc tacgcggttc atgactaccc
     3001 gcgagtccgc ttcgactacc gctttgagac agccggctgc aatctgaaca tcgtctccgt
     3061 ggtgaacggc gacctgcaga cgatcggctt cgcgggcgag aacggcgcct atcctccctt
     3121 tgtgcagaac aacattggcc ggatcaaggg tgtgaagcag gacggtcagt ggcacgctgc
     3181 ggagtttgat tttggcgccc tcctgcgccg gcgctatccg aacatgccgc gcttcttcgc
     3241 cgactacctg ggcacctggg caaccggggt tcgctccact tacgataacc cgggcggcgt
     3301 gagcctctgg ctcgacaaca tcacgatcta ctcgccgcgg gcgacctcgg ccgcgttcga
     3361 gtggcagcca ccggcggatc ccaacggaat tcgcggctac tcctatgtcg tcgatcagaa
     3421 gcaggagacg ctgccgaacg agaggatcgt gacgcgcgag ccgcgctgcc atgtggagaa
     3481 tctgaagccg ggcgcctggg tcttccatgt gcgtgcttgc gatggcgccg gcaactgggg
     3541 tccggcggcg cacctcccct tccagctgac gcaatagtat gggtgggaga gcgtacgcag
     3601 ggcgtcgctc gcgccagccg caacgggaga agcgcgcgcc tgcgcgtgct caagctggcg
     3661 gtgtgacgaa gacgtactcg ccgggctgct gggggtcatc caaccgacgg attgccatga
     3721 gcgcgatgtc gtctcgcaga ttgccggcgc tgtgggcagc cgcagcgtca tacagggtgt
     3781 tcaggatggt cgacgggcct tccgcggagt gcgccgcgag agtcgctgca acgcgctcta
     3841 ccccaaacag atcgggatct cccgggcggt gcgggtccac cagaccgtcg gtgtagagca
     3901 ccaggatatc gccaggggtt agaaccgtct cacggctatc ataggggccg ttggcggcag
     3961 ggatcaacgc ggagccgtgg gttgtgagct gctccacgcg ctgctcgcgg gcgcgccaca
     4021 ccaaggcggg ctcatgtcca gcggaggcat aggtcaatcg gcctgtttct cggtccagga
     4081 aggccaagaa gagggtggcc aggcggtccg ggtcgccagc ggcggcgaag gtctgttgga
     4141 tgccatcgag gatgatttcc ggatggcccc tctcatggag cgccagagca gagatgccat
     4201 gctgcatgcg cgccccgata accgccgcgt ccaggccctt cccgccgaca tccccgatca
     4261 ccagacagag gtagcgatcg tccattggga agatgttgta gaagtcgccg ccgacatccg
     4321 cttcgataag cgccggtcgg taagtgtggg caatcgcgta gccgggtacc gcggcacatt
     4381 gatccgggag cagggcggcc tggaaccgct ccgcgatgcg ccgctcgcgt ccgagtgcct
     4441 ggcgggtctc ctcttgagtg gcgcggagcg cattcagcac gcgtttgcgc tcggtgatat
     4501 cctcggctat gatgaggatg ccggtggtcc gcccggaagc atcgggcagg ggtgctgccg
     4561 agaggctcaa gtcgatcacg tgatagtcac cgctagcgat gcgtgtctcc agcccgtgga
     4621 tgactccgcg agtgcaaacg cgctccgcga gcagcgtggg atcttcatcg tctgccaggg
     4681 gaagtagccg tccgagcatc tgcggagact gccagccgaa ggctcgccgg gcagccgggt
     4741 tccagccgcg aatatgcccg ctccaatcga gggtgacgat cggcaaggga gaggcttgga
     4801 tgatagcgcg gagcgccgtc tgcgtccgcc gcaggctatc actcaaggag ctgatcagaa
     4861 tagcgactcc catgaagaac gcgaggcgca cgagttcatc cacgcgccat ggggtcgtgg
     4921 gatcgcgtgg ctccacatat tggtggatga gccaggcaga gagcgcagtc gagagcaagc
     4981 caggcagaag gccgccaaac caggcgctga ttgctacggc tgcgaagaag aggacgaaga
     5041 ctgtcggcgc gacgagatcg cgagccgcca gcgtgatcgc gagcgcggcg agcgtggatc
     5101 ccaaagcgat gagccagcgg agtgtgtcgc gccattgagg tgccgcgggg gtgccattgc
     5161 agcaaggtgc acgaggggcc ctgcgcgttt cgaccgcctc ggttgtaccc tccgtcgatg
     5221 ccgtgggatc catcaggcct tcccctgcac aagatcagtc gccctggccg catcaccagg
     5281 aaaaaggccg gccgcgaatt tggccacgcc aatggcgcgt tgttgctatg aaagggggta
     5341 tggaaggctg ccttccatag tgaattccag ccagcgcgcg gcggcaacat cgcgggtctg
     5401 cgtcaggtgg gttggagcc
//
LOCUS       DAOSCV010000188         5362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9677_length_5362_cov_2.991709, whole genome shotgun sequence.
ACCESSION   DAOSCV010000188 DAOSCV010000000
VERSION     DAOSCV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9677_length_5362_cov_2.991709"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLY56_06025"
     CDS             complement(<1..182)
                     /locus_tag="PLY56_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21072.1"
                     /translation="MPEATRCFWAKPLRGNLSHPALLRLRRECGQVQPGRASLASSAV
                     IYRRAVPGGVACVQVTR"
     gene            complement(247..2181)
                     /locus_tag="PLY56_06030"
     CDS             complement(247..2181)
                     /locus_tag="PLY56_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21073.1"
                     /translation="MTLSTRAIFLGLVAVAVVVFIVSWAELVTGQIMIGFLQLPPVVL
                     ALLFLLVLVNRGVARWGGKMALNPQELAVIHVMMLLAAMISSRGLMEKLVPTLVGQNY
                     FADSTNNWASLYFQHIAQWLVPWDVKGGEKQWLAVRFFEGLRSWETLPWIAWLRPMLA
                     WSVLVLAMFFAYLCLATILRKQWVENEKLSFPLAQLPLELIRGGEGSFFSSRIFWIGF
                     AIPFTIFTINGLHNLFPTIPGVTTQINVKQYLTNRPWSMVSFFMAFLSPAAIGFFFLL
                     PTDLLFSLWFFFILGKVQEASYIQLGLVPESTPHGGAHLPVGAQSTGAWFALAAYLLY
                     VSRPHLKRVWHAARTGRDEGSRGELLSYRTAVFGLAFSFVVMIVWAVAAGMSPWLAFF
                     QLAVFLFVQAIIMARSTSERGLLMTEGTFVPADLLVAGMARNTLGPQNLTVLAFLDGM
                     FTRDLRGILLTGFLDGQRVADGVGLSRRRLLTACTLAVVVAMVLAGALHIWLPYTKGG
                     LNLYSYIYRSHSIQFFRENQVAMQGTVPTKWYYLGWVGCGVAVTAFLGWMRMRFYWWP
                     LHPLGYALTSTWTILVFWCPILIAWVCKASISRYGGMAAYQRARPFFLGMVFGEFTAA
                     MGWTLVAMATNAPAPAFPWP"
     gene            2521..5196
                     /locus_tag="PLY56_06035"
     CDS             2521..5196
                     /locus_tag="PLY56_06035"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ21074.1"
                     /translation="MKHPILIILGGLMFLPVAALPGAPAISSHRDLYRLPLPEKLPPH
                     PRLFCTSADLERIHRDAAAGDPYTRATIERIASEAEAALARPLPEGKPSPADFRAAAI
                     LGQGYALTANEAFGRRCRDMLLAFAAICPTLKTTRAAGRFTESTLLEGPVAVNAAMAY
                     DLVAGAPFMSAPDRKRIEEDLLRILAWECGHRCHHRNSSNWRTWALAIIASCGFAIGD
                     RALIEEAVNGIWDPERNAYLYGAVQTLTHSIFSDGIHWERSIGYTYYTASALMYVMVA
                     AKNSGIDLWHAKLPGILGPFEGCAPHEEFGPPGDRSIRSFLDAPFFYAFPNGLIARVG
                     DSGTRELAYHPIYELAYREYHDPRYAWLIRRHRKGYGRAAAAGWNVWTPSGHPTAALA
                     EGEGRLGRNAFRLKCAAGDRAALVQDVRMPADRPARVTAWVRALAMDGGSAHIRCNFD
                     STFLFTERVKQPGDWRQLAVEIPARPGAKPGDQRSIRLHLFLEEGAGEVLWDDVTVTA
                     DGVPGNLAINPGFDETTVDDRALDFWSLVHAPREVPEGRYSLDDDVTIGLSGRHVNGC
                     TLFPVGGFAILRQRPATPESTAVNFTFGPYGSGHDHPDRLHFDLYGLGEILMPDAGSW
                     GYNNPMHVTWANQSIAHNTLTVDEVAQEPQGTSLSVFAAERGDQRVFGVLRLFHPGNL
                     LRAARATCDTAYPGVSMDRTLCLVGSYVLDVFRARSDAEHTYDLAFHGLGDVSTEAPL
                     KPAETFTATGYAHLTQVRRGVPGGSSFRADFRAGERALLLVQAQPAGAEVILAKDPDR
                     GTPTSCCIARRRGKNALYVTVLEPYTSAPTVRNLSVESGDSAVTVTVEHASGKDLFAL
                     ENALDGRITLRPIDATGQARSEQTGPGSRTGSPPM"
BASE COUNT          985 a         1775 c         1751 g          851 t
ORIGIN      
        1 cgcgtcacct ggacgcaagc cacgccccca ggcaccgcgc ggcggtaaat caccgcgctg
       61 cttgcgagcg aagccctgcc gggctggacc tggccacatt cgcggcgcaa gcgcagcagc
      121 gcgggatgag agaggttgcc ccgcaggggc tttgcccaaa agcagcgcgt agcttccggc
      181 atcgcacctg cgtgagcccg tctctgcccc cttcatccgt ggcatcgatg gcccggcgca
      241 ggcccactat ggccatggga aggcaggtgc cggcgcgttg gtcgccatcg cgacgagcgt
      301 ccatcccatg gcggcggtga actcgccgaa gaccatcccc aggaagaagg ggcgcgcgcg
      361 ctggtaagct gccatgccac catagcgcga gatactcgcc ttgcagaccc aggcgatcag
      421 gatggggcac cagaagacga ggatggtcca tgtcgaggtg agcgcgtagc ccagcgggtg
      481 gagcggccac cagtagaacc gcatccgcat ccatcctagg aacgcggtca ccgccacgcc
      541 gcaccccacc caacccaggt agtaccactt cgtgggcacg gtcccctgca tcgccacctg
      601 gttctcgcgg aagaactgga tgctgtgcga gcgatagata taggagtaaa ggttgaggcc
      661 gcctttggtg tagggcagcc agatgtggag cgccccggcc agcaccatcg ccacgaccac
      721 cgccagcgta cacgccgtca gaagccgacg gcgcgagagg ccgacgccat ccgcgacgcg
      781 ctggccatcc aggaaaccgg tgagcaggat gccgcgcagg tcgcgcgtga acatgccatc
      841 gaggaaggcg agtacggtca ggttctgcgg ccccagggtg ttgcgcgcca ttcctgccac
      901 caggaggtcg gcgggaacga aggtgccctc ggtcatgagg aggccgcgct cgctggtgct
      961 cctcgccatg atgatcgcct ggacgaagag gaagacggcc agctggaaga acgccagcca
     1021 gggcgacatc cccgcggcga cggcccacac gatcatgacc acgaaggaga atgccaggcc
     1081 aaagaccgcg gtccggtagg agagcagctc accgcgagaa ccctcgtcgc gccccgttct
     1141 ggccgcgtgc cacacacgct tcaggtgggg ccggctcacg tagaggaggt aggccgccag
     1201 ggcaaaccag gcgcctgtcg actgagcgcc caccgggagg tgcgctccgc cgtgcggcgt
     1261 gctctcaggc accaaaccga gctgaatgta gctcgcctct tgcacctttc ccaggatgaa
     1321 gaagaaccag aggctgaaga gcaggtcggt gggcaacagg aagaagaagc cgatggcggc
     1381 cggcgagaga aacgccatga agaagctgac catgctccag ggccggttgg tgaggtattg
     1441 cttcacgttg atctgcgtcg tcaccccggg gatggtggga aagaggttgt gcagaccgtt
     1501 gatggtgaag atcgtgaagg ggatcgcgaa gccgatccag aagatccggc tgctgaagaa
     1561 ggaaccctcg ccgccgcgga tcagctccag aggcagttga gccagcggga aggagagctt
     1621 ctcgttttcg acccactgct tgcgcaggat agttgccagg cagaggtagg cgaaaaacat
     1681 cgccagcacc agcaccgacc atgccagcat cgggcgcagc cacgcgatcc agggcagcgt
     1741 ctcccaggaa cgcagcccct cgaagaagcg cactgccagc cactgcttct cgccgccctt
     1801 gacgtcccag ggcaccagcc attgggcgat gtgctggaag tagagcgagg cccaattgtt
     1861 ggtgctatcc gcgaagtagt tctggccgac cagcgtgggg accagcttct ccatcaaccc
     1921 gcgcgaggag atcatcgccg ccagaagcat catcacgtgg atgacggcca gctcctgcgg
     1981 gttgagcgcc atcttcccac cccagcgcgc gacgccccgg ttcacgagca ccaggaggaa
     2041 gagaagcgcc aggacgaccg ggggcagctg gaggaagccg atcatgatct ggcctgtcac
     2101 cagctccgcc caggagacga tgaagacgac aaccgccacg gctaccaggc cgaggaaaat
     2161 cgcgcgcgta gagagtgtca cgtcgcgtgc tgctccttca gtgtggaagt gtgggcgtag
     2221 cgccatggtg caacaacggc gtgtctcgtg gaaccggtct cgttgggtct catcactgga
     2281 ccgcgggtcg cccgctccgg caatcgaacg cccatcgagg cggagggcgg acctgttgca
     2341 ccaggttgca tcacgacacg cctcattata tcggccttgg agcacgcggt caacctctgg
     2401 atgacatccg gttgggctct ctcgtgccgc gctggcatcc gagtgctaca ggacggagcc
     2461 gcctctcccg cgaaatgagt tacgacattc cgggggcgtg cgcgaaaggg agtatggcgt
     2521 atgaaacacc cgatcctcat catcctggga ggcctgatgt tcctgccggt tgccgcgctg
     2581 ccaggggcgc cggcgatctc ctcgcaccgc gacctttacc ggctgcctct cccggaaaag
     2641 cttccgcctc acccacgtct cttctgcacc tctgccgatc tggagcggat ccatcgagat
     2701 gcagcggccg gcgatcccta cacacgcgcc acgatcgagc ggattgccag cgaggcggag
     2761 gcagcgctgg cccggccgct cccagaaggg aaaccatccc ccgccgactt ccgcgccgcg
     2821 gcgatcctgg ggcaaggcta cgcgctcacc gcgaatgagg cgttcgggcg aaggtgccgc
     2881 gacatgctgc ttgccttcgc ggcgatctgc cccacgctga agaccactcg cgccgcaggg
     2941 cgtttcaccg agagcacgct gctcgaaggt ccggtggcgg tcaacgcggc gatggcgtat
     3001 gatctggtgg cgggcgcgcc cttcatgagc gcaccagacc ggaaacgcat cgaggaagac
     3061 ctcttgcgga tcctggcgtg ggagtgtgga caccgctgtc accaccgcaa ttcgagcaac
     3121 tggcgcacct gggcgctcgc catcatcgcg tcgtgcggtt tcgccattgg cgaccgcgcg
     3181 ctgatcgagg aagcggtgaa tggcatctgg gatccggagc gcaacgccta cctctatggc
     3241 gccgtgcaga cgctgacgca ctccatcttt tccgacggca ttcactggga gcgcagcatc
     3301 ggctatacct actacaccgc gtcggcgctg atgtacgtga tggtggcggc gaagaactcc
     3361 gggatcgacc tgtggcacgc gaagctgccc ggcatcctcg gccccttcga ggggtgcgcg
     3421 ccgcacgagg agttcggtcc tcccggggat cgctcgatcc ggtcgtttct ggacgcgccc
     3481 ttcttctatg cattccccaa tgggctcatc gcgcgcgttg gcgattccgg cacgcgcgag
     3541 cttgcctacc accccatcta tgaactggcg tatcgtgagt atcacgaccc gcgctatgcc
     3601 tggctgatcc gccggcatcg gaagggctac ggacgtgccg cggccgcggg atggaacgtc
     3661 tggaccccat cgggccatcc cacggcagct ctcgccgaag gagagggccg acttggccgg
     3721 aacgccttcc gactgaaatg cgccgcaggc gaccgcgcag ccctcgtcca ggatgtgcgc
     3781 atgcctgcgg ataggccggc gcgggtgact gcctgggtgc gcgcgctcgc gatggatggg
     3841 gggagtgcgc acatccggtg caatttcgac agcaccttcc tcttcaccga gcgcgtgaaa
     3901 caacccggcg actggcggca gctggccgtc gagatcccgg cacgcccggg tgcaaagccg
     3961 ggcgaccagc ggagcatccg cctccatctc ttccttgagg agggcgccgg cgaagtgctg
     4021 tgggacgatg tcaccgtcac cgccgatggc gtgccgggca atctggccat caaccccggg
     4081 ttcgacgaga caaccgttga tgaccgtgcc ctcgatttct ggagcctggt tcacgctccc
     4141 agggaggtgc ccgaggggcg ttactccctc gatgacgacg tgaccatcgg cctcagcggc
     4201 cgccacgtca acgggtgcac cctcttcccg gtcggcggtt tcgccatcct gcggcagcgt
     4261 cccgcgacgc ccgagtcgac cgcggttaac ttcacctttg gcccgtatgg cagcgggcac
     4321 gaccatccgg accgtctcca cttcgacctg tatggcctgg gggagatact gatgccggat
     4381 gccggctcgt ggggctataa caacccgatg cacgtcacgt gggccaacca gagcatcgcg
     4441 cacaacacat tgactgtgga tgaggtggcc caggaaccgc agggcacctc gctcagtgtt
     4501 ttcgcggcag agaggggcga ccagcgcgtt ttcggggtct tgcgcctctt ccacccaggt
     4561 aatctcctcc gggcggcacg cgccacctgc gataccgcct acccgggagt ttcgatggac
     4621 cgcactctct gcctggtagg gtcgtacgtt ctggacgtat tccgggcacg ctctgatgcc
     4681 gagcacacct acgatctcgc cttccacggc ctcggggatg tgagcacgga ggccccgctg
     4741 aagccagcgg agacgttcac ggccaccggc tacgcccatc tgacccaggt gcgccggggc
     4801 gtgccaggcg ggtcatcctt ccgtgccgat tttcgcgctg gcgagcgcgc gctgctcttg
     4861 gtgcaggctc agcccgccgg ggctgaggtg atcctggcaa aggatcccga caggggcacg
     4921 ccgacgtcgt gttgcatcgc gcgccggcgc ggcaagaatg cgctctatgt caccgtgctg
     4981 gaaccgtaca cctcggcacc caccgtgcgt aacctgtcgg tggaatcggg cgatagcgcc
     5041 gtgacggtca ccgtggagca cgcctccgga aaggacctct tcgccctcga gaacgccttg
     5101 gatggccgga tcaccctccg tcccatcgac gccacggggc aggcgcgctc ggaacagacg
     5161 ggcccgggca gccggacagg ttctccacca atgtaacggg ccgcgcgggc gtcccctcgc
     5221 gtgagagagg aatggagatc atgcgaattg gagccatcat agcgatgcta ctcgtgttcg
     5281 cggcgacgca gaccgccgcg gatccggaaa acctcgcggc aggcaagccg gtccgcttcc
     5341 tgccaccccc gaactacgct ct
//
LOCUS       DAOSCV010000189         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9693_length_5354_cov_2.990753, whole genome shotgun sequence.
ACCESSION   DAOSCV010000189 DAOSCV010000000
VERSION     DAOSCV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9693_length_5354_cov_2.990753"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLY56_06040"
     CDS             complement(<1..373)
                     /locus_tag="PLY56_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21075.1"
                     /translation="MHSKWTRVTKRGTLHCNETWEGDRRRTTLQLAAPGQEPVTPDLV
                     LWADSDHGVVVCQQVVLPDAPAHVMADTLRRAIDHPAPGGGPPRQPRCIRVNRQEIAE
                     AVRPVTDPLGIRVEVTDTLPLA"
     gene            672..748
                     /locus_tag="PLY56_06045"
     tRNA            672..748
                     /locus_tag="PLY56_06045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:706..708,aa:Val,seq:gac)
     gene            811..3252
                     /locus_tag="PLY56_06050"
     CDS             811..3252
                     /locus_tag="PLY56_06050"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOJ21076.1"
                     /translation="MSFCSRAEVLACGRFPVNLILGAQCRYRIPILLVAGSLATHTAH
                     CSPYPSGRVQSVNPTPLGTVYLVLLGLAIAIAIGMMGMAWKRRTQRCAPSLLVLVSLV
                     ALLAAASALEAIVPRIECKEFWVNIQYLGYQLIPIAYLTMVFQYVGFQRWLAPSRILL
                     ISAVPLITVILAWTNDYHLLMRRDLFIEPAEGLGFLGKTYGPWFSLASVYSYLITLCG
                     LTLLTWASRRAASVYRRQALLLIIGTVLPLAWSLAYVLRLPLLPPIDFMPPITALSLL
                     LIALAVLRFRLLEIVPVACHQVVQAMEDGLLVLDDERQVAAINPAAARMLSVTPSAAV
                     GEPATQILEAFPQVLAPILAETPPNSIVVSVGTPDRAAYWEVRISELRSERGHPLGQA
                     VILRDVTEQKQGEKARRAIEARMLQAQKLESLGVMAGGIAHDFNNLLQVILSNTDLIR
                     IALPEDHSTQKLLRGIIRSTERAASLASQMLAYSGKGRFTLEQLDPARLVSGMADLLR
                     SLAPKKVALALEISTDIPSIHADAAQIRQLIVSLVSNAAEAIGEREGTITLRLAQVHA
                     TRQLLAPLHQSGELPEGNYVQLTITDDGCGMDEATQARVFEPFFTTKFTGRGLGLAAA
                     LGIVHAHRGAIGIKSAPGRGTTVTVLIPGAGASPPVQEVGIPLLCERQASGTILVIDD
                     EAGIRDTAMMALRSYGYRVLAASDGLEGIDLFREHQNEIDAVVLDLAMPRMSGEEVVP
                     ALRGLRSDVPIILTSGYPEEEALRRFAGRDLSGFLQKPYRPHLLAAQLDTLIARTIRP
                     REAVAHINEGGKPHE"
     gene            3245..3694
                     /locus_tag="PLY56_06055"
     CDS             3245..3694
                     /locus_tag="PLY56_06055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOJ21077.1"
                     /translation="MSEAASQHTPCRPPLACEMPLPVRIYDTDLLGVVNNVVYIRWLE
                     DLRTAWCDRYWPLTEQMAAGYAPVIVSITIDYLSPVRFGDHVIGRVWVTPRKARLVFD
                     TEIHANGRPAATARQIVALVHLDGMKPTRIPHWLIERYEEALQAANP"
     gene            complement(3771..4406)
                     /locus_tag="PLY56_06060"
     CDS             complement(3771..4406)
                     /locus_tag="PLY56_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21078.1"
                     /translation="MAAVSVPGCAPNETLHRERPPVNQIRAEVLAGGQPLSGVTVALA
                     GTESRTETTGPNGIAAFDGLPAGSFTVTISGFDQSRWRFVRTSTPVTLGAPIGQVTVQ
                     FRGDPLPTGAITGRVTVGGRPLSQLQGESISLTLVPPEGDEVTRTLAGSDGTFAFTGL
                     REGEFTLRLAYRGQTRALTVTLARGERRILSLDLLLPGGERRSADDSGVPR"
     gene            complement(4734..>5354)
                     /locus_tag="PLY56_06065"
     CDS             complement(4734..>5354)
                     /locus_tag="PLY56_06065"
                     /inference="COORDINATES: protein motif:HMM:NF037639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HOJ21079.1"
                     /translation="SVAEGQQLQANLEFASLPSFNQGPSGEPTGQVRAQVTAAGTPLS
                     GVTVTLNGAAQRVATTDASGLAVFQNLPDGTYTVSIAGWDASQFLFTTTSTTVTLDPG
                     VGLVTAQFQGVAAANGTLTGLVTINGQAPADIPDQTVTITLVPPVGNALTGDLTGTTG
                     EYTFAQLQPGVHTVRVAVGGQVRSTTVTLAQGERRELNLEFNQLTP"
BASE COUNT          899 a         1847 c         1617 g          991 t
ORIGIN      
        1 cagcaagggg gagtgtatcc gtgacctcca cgcgaatgcc cagaggatcc gtgactggac
       61 ggaccgcttc ggcaatctcc tggcggttca cgcggatgca gcgcggctgc ctggggggcc
      121 ccccgccggg cgccggatgg tcaatggcgc ggcgcagcgt atcggccatc acgtgggcgg
      181 gcgcgtccgg gagcacgacc tgctggcaga cgacgacgcc gtggtcggaa tcagcccaga
      241 ggaccaggtc gggagtgacg ggttcttgtc cgggagcagc aagctggaga gtcgtccggc
      301 gccggtcgcc ctcccacgtt tcgttgcagt gtagtgtgcc gcgcttcgtg actcgggtcc
      361 acttgctgtg cattgccgtc ctcctccctc tcattgtacc ccaccccgcg gcgattgtcc
      421 atcccccaga gcgatacctc tccacgcacg gctgtctggt gcgcgtctga ggcattccct
      481 cattcggttc ggcatcggac cacactcccc cttcgcggtg tatcgaccac ggatcatggt
      541 ctcggcgctc gcaccgattt ggtgagtgcc agggcaaatc ccaccccctt tcggatggga
      601 ttgtaatgaa atatgacttc gatctggggt tgacaaaaat ggggtatcac gctataatgt
      661 gcatggttgg cgggcggtta gcccagttgg ttagagcgct tgcatgacac gcaagaggtc
      721 acaggttcga gtcctgtacc gcccaccatt ctctctctaa tagatgctgg aggcaactac
      781 ccatcctcct gtcttagaca gggagcggta atgtcgtttt gttctcgggc cgaagttctg
      841 gcttgtggcc gcttccctgt taatttgatc ttaggcgcgc agtgccgata ccgcatccct
      901 atccttctgg tcgcaggttc cctcgccacg cacacggccc attgcagtcc ctacccctca
      961 ggtcgagttc aaagcgttaa tccaaccccc cttggtaccg tttacctggt ccttctggga
     1021 ctcgccatcg cgatcgccat cggcatgatg ggaatggcat ggaagcggcg gacacagcga
     1081 tgcgcgccgt cactcctcgt tctcgtctct ctcgtagctc tcctcgccgc agccagtgcc
     1141 ctggaagcca tcgtcccccg catcgagtgc aaggagttct gggtcaacat ccagtacctg
     1201 ggataccagt tgatccccat cgcctatctg accatggtct tccagtacgt tggcttccag
     1261 cgatggctcg cacccagccg catcctgctc atcagcgcgg tccccctcat cactgtgatc
     1321 ctggcgtgga ctaacgacta ccacctgctc atgcgacgcg acctcttcat cgagccagca
     1381 gaggggcttg gctttctagg aaaaacctac gggccgtggt tttctctcgc cagcgtctac
     1441 tcttacctca tcactctctg cgggctcact ctcctgacct gggccagccg gcgcgctgcc
     1501 tcggtctacc gccggcaggc actcctcctc atcatcggca cggtgctacc gttggcctgg
     1561 tcactcgctt atgtgctgcg ccttccgctg ttgccgccca tcgacttcat gccccccatc
     1621 acggcgctca gcctgctcct catcgccctc gccgtccttc gcttccggct actagagatc
     1681 gtgccggtgg cgtgccacca ggtcgttcag gcgatggagg acggcctcct cgtccttgat
     1741 gacgagcgcc aggtcgcggc catcaacccg gcggccgccc gcatgctctc agtgactccc
     1801 tccgcggcgg tgggcgagcc cgcaacccag atactcgaag cgtttccgca ggtgctcgcc
     1861 cccatcctcg ctgagacacc ccccaactcc atcgtggtgt cggtgggcac cccggaccgc
     1921 gccgcgtatt gggaggtgcg gatctcggag ctgcgctccg agcgagggca ccctttgggg
     1981 caggccgtca ttctgcgcga cgtcaccgag cagaaacaag gcgagaaggc aaggcgcgcg
     2041 atagaggcac gtatgctcca ggcacagaag ctggagagcc ttggggtgat ggccgggggc
     2101 atcgcccacg atttcaacaa cctgctccag gtgatcctca gcaacaccga cctgatccgg
     2161 atagccctgc cggaagacca ctccacccag aaactgctgc ggggcatcat ccggagtacc
     2221 gagcgcgccg catctctggc cagccagatg ctcgcctact ccgggaaagg gcgcttcacc
     2281 ctcgagcagc tggacccagc gcgcctggtg tcggggatgg ccgatctgct ccgttctctc
     2341 gcgcccaaga aggtggcgct cgccctggag atctcaaccg atatcccttc gatccacgcc
     2401 gacgcggcgc agatccgcca gttgatcgtc agcctcgtct ccaatgccgc cgaggcgatt
     2461 ggcgagcgcg aaggcaccat caccctccgg ctcgctcaag tccatgcaac acgccagctc
     2521 ctggctccgc ttcaccaatc gggcgagctg ccagagggca actacgtgca gctcaccatc
     2581 accgacgacg gctgtggaat ggacgaggcc acgcaagccc gcgtcttcga gcccttcttc
     2641 accaccaagt tcaccgggcg cgggctaggc ttggcggcag ctcttgggat cgtgcatgct
     2701 caccgcggtg ccatcggcat caagagcgcg ccggggcgcg gcacaacggt caccgtcctc
     2761 atcccgggtg ctggtgcgtc tccgcccgtt caagaggttg gtatccctct cctttgcgag
     2821 aggcaggcaa gcggaaccat cctcgtgatt gacgatgagg ccggcatccg cgacacggcg
     2881 atgatggcgc tacgcagcta tgggtatcgt gtcctcgctg cttcagacgg ccttgaggga
     2941 atcgacctct tccgcgagca tcagaacgag atcgacgcgg tggtgctcga cctggcgatg
     3001 ccgcgcatga gcggcgagga agtggtgcct gccctgcgtg gcctccgcag cgatgtacct
     3061 atcatcctca caagcggcta tccggaggag gaagcgcttc gccgatttgc tggaagggac
     3121 ctttcgggct tcctccagaa gccgtaccgg ccgcatctcc tcgcggcgca actcgacacg
     3181 ctcatcgcgc gcaccatcag gccacgggag gcagtggccc acatcaatga aggaggaaag
     3241 ccgcatgagt gaagctgcaa gccagcatac cccctgcaga ccaccactcg cttgcgagat
     3301 gccgttgccg gtgcgcattt acgacacgga tctcctcggc gtcgtcaaca acgtggtcta
     3361 catccgctgg ctggaggacc tgcgcacggc ctggtgcgac cgctactggc ccctcaccga
     3421 acagatggcg gccggctacg cgcccgtcat cgtctccatc actatcgact acctcagccc
     3481 cgtgcgtttc ggcgatcacg tcatcggccg cgtctgggtc accccgcgga aagcacgcct
     3541 ggtctttgat acggagatcc atgccaacgg ccgccccgct gcaaccgccc ggcagatcgt
     3601 ggcgttggtc cacctggacg ggatgaagcc cacccgcatc ccccattggc tgatcgagcg
     3661 gtatgaggag gccctccagg cggccaaccc gtaatcctct tcgctccccg acgatctgcc
     3721 ggtcgccggt cgcacctgga gtgagccaca cccgcccggg atagcatccc ctatcgaggg
     3781 acgccactat cgtcggcgga ccgtcgctcc ccgccaggca agaggagatc gaggctcagt
     3841 atgcggcgct cgcctcgggc aagcgtcacg gtgagtgctc tggtctggcc acggtacgca
     3901 aggcgcaggg tgaactcgcc ttcccgaagc ccggtgaacg caaacgtgcc gtcgcttccc
     3961 gcaagcgttc gggtgacctc atcgccctcg ggaggcacaa gcgtgaggga gatactctct
     4021 ccctgaagct gcgagagggg gcggccaccc accgtcaccc ggcccgtaat cgcgcctgtg
     4081 ggcaggggat cgccgcgaaa ttgcaccgta acctgaccga tcggggcgcc gagcgtcacg
     4141 ggggtagacg tgcgcacgaa gcgccagcgg ctctggtcga aacccgaaat ggtgacggtg
     4201 aatgagcctg ccggcaggcc gtcgaacgcg gcgattccgt tggggccggt cgtctccgtg
     4261 cggctctccg tgccggccaa cgcaaccgtc accccggaaa gcggctggcc gcccgcaagc
     4321 acctcggcgc gtatctggtt gacgggcggc ctttccctat ggagagtctc gtttggggca
     4381 catcccggca cgctgacggc tgccattccg atcaccacgg ccgcgcgcca tctccatctc
     4441 ttggcgcatc gcgcgcccct cttcctcacc gccacgcggg cggtcttcag cgcgcctgct
     4501 tcacgcgctg gtgcggcact gctgccgcat cggagcatac cgaaggcctc ccttccgata
     4561 cccgccggca caccacccgc ctcgtcagac tgcatcccga aagaatgcgc cctttggacg
     4621 ccggccggcg cggaccggac cggtgtatgg ggcgaagcat tcgcgggcac acggcccacg
     4681 ccaatgcgcc cgatgcgaat gcttcgcccc cgccagcacc cccatgcgca aagttacggg
     4741 gtgagctggt tgaactccag gtttagctcg cggcgctctc cctgtgccag ggtgacggtg
     4801 gtggatcgga cctgaccgcc aaccgcgaca cgcaccgtat gtacccctgg ttggagttgc
     4861 gcgaaggtgt actccccggt tgttccggta aggtcaccgg tcagcgcgtt gcctacgggc
     4921 ggcaccaggg tgatcgtcac ggtctggtcc gggatatccg ctggcgcctg accattgatc
     4981 gttacgaggc cggtgagcgt gccgttagcc gctgccacgc cctggaactg cgccgtcacc
     5041 aacccaacgc ctggatccag cgtcaccgtg gtgctcgtcg tagtgaagag gaactggctg
     5101 gcatcccacc cggcgataga gacggtgtag gtaccatcgg gcaggttttg aaaaacagcc
     5161 aggccgctcg cgtccgtcgt cgccacccgc tgggccgcgc cattcagggt caccgtgacc
     5221 ccggaaaggg gcgtgccggc ggcagtcacc tgagcgcgca cctggcctgt cggctcgccg
     5281 ctcgggccct gattgaaaga cggcaggctg gcgaactcca ggttcgcctg aagttgctgt
     5341 ccttccgcga cgct
//
LOCUS       DAOSCV010000190         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9712_length_5345_cov_3.395274, whole genome shotgun sequence.
ACCESSION   DAOSCV010000190 DAOSCV010000000
VERSION     DAOSCV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9712_length_5345_cov_3.395274"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PLY56_06070"
     CDS             complement(<1..357)
                     /locus_tag="PLY56_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOJ21080.1"
                     /translation="MEERVRCAVLPNGIRVLTEEIPHVLSATVGIWVGVGSVHEPPAL
                     AGICHALEHMLFKGTATRSARDIAETLDTVGGQLNAFTDKEATCYHARVLAEHVPLAI
                     DLLCDMLLNSRLHPADL"
     gene            complement(500..2767)
                     /locus_tag="PLY56_06075"
     CDS             complement(500..2767)
                     /locus_tag="PLY56_06075"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ21081.1"
                     /translation="MPTVVEATLGEATVSIETGRVAKQAGGAVLIRSGDTVVLVTATA
                     SQEPREGVDFFPLTCDFEERMYAVGRIPGGRIKREGRPSEFAVLTSRLIDRPIRPLFP
                     TGLRNEVQIVATPLSVDQDHPPDVLAVTGASAALAVSDIPFPNLIAAVRVGRVDGQFI
                     INPSWAQAEEGDLDLVVAGTKTSVVMMEAGAKEVTEEDLLAALEFAQEHIRALIAAQE
                     ELAAKAGKPKRDFPSFIVDPELIDAVREREATRIREAIQEPDKAAREAGLQLLRGEIV
                     AELAPDFPEREAELSEAVDKVIKEELRALILDEGKRPDGRGVEEVRPITAEVSLLPRT
                     HGSALFTRGQTQVLATTTLGSPGEGQKLEGLELALEQKHFMLYYNFPPYSVGETRVLR
                     GPGRREIGHGALAERALQPMLPPDSEFPYTMRVVCEVLESNGSSSMGSVCAGCLALMD
                     AGVPMRKPVAGVAMGLVTRGDQYVVLTDIQGMEDFTGDMDFKVAGTRDGITAIQLDIK
                     IEGLSMEMLANALAQAKRGRMHILEKMVDVLPAPREELSEYAPRIFILEIHPDKIGEV
                     IGPGGKIIKKITAETGATIDIEQDGRVFITSVEAAGGEMARKMIDDITRDVRVGETYL
                     GKVVGIQSFGAFIEILPGREGMVHISELAHRRVARVEDVVKIGDEVMVKVIDIDPQGK
                     IKLTRKGLLEPEEGEAAAPAEAPRGERPSGGQRGGGQRGGGQRGGGQRGGGGGGRPGG
                     EERPSAKFRPKERRG"
     gene            complement(2940..3203)
                     /gene="rpsO"
                     /locus_tag="PLY56_06080"
     CDS             complement(2940..3203)
                     /gene="rpsO"
                     /locus_tag="PLY56_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ21082.1"
                     /translation="MQPSQKQGIIAQHKKHEQDTGSPEVQVALLTARINQLTEHLRTH
                     KKDHHSRRGLLMMVGHRRRLLNYLSNKDIERYRALVAALGLRR"
     gene            3649..>5345
                     /locus_tag="PLY56_06085"
     CDS             3649..>5345
                     /locus_tag="PLY56_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ21083.1"
                     /translation="MSIVDDLGRAPSTDVATVVDIPNLIEIQTASYHWFLKDGLRELF
                     HNFSPIEDPTGTYALYLDDYLLKDAKYTIEECRDRDITYERPISARIRLLNKRTGEIM
                     EDEVYLGDLPVMTEKGTFVINGAERVVVSQLARSPGVYFRDNIDYSGRVLFSAQVIPA
                     EGAWIEVETDANDVIFVRIGQARKFPITTLLRALNAFDHELGETQRPQTATTYQIYRL
                     FGTVRVVENPTVENLISFDAGPVIRIYQDVTDPNSGKVLAKAFDKLDRPLAEKIEAAG
                     IQNIEIIEIPRYIEATIEQDAVTEKENPQRAEDEALLDIYRKVRPGDPATRESARALL
                     SSIFFDIKRYDLARVGRYKMNKKLGIDLPLTVRSLTKDDLVAIVRYIIKLSHNEGSVD
                     DIDHLENKRVRSVGELLQSQFRLGLLRMEKVAKERMTSLEPDTVSPQAVVSVKPISAA
                     IKSFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLSRQSAKLEVRDVHHSHYGRIC
                     PIETPEGPNIGLIGSMAMHARINEFGFLETPYRKVVDGKVTDQIDYLTADEEAPYHIA
                     "
BASE COUNT          980 a         1736 c         1654 g          975 t
ORIGIN      
        1 gagatcggcc gggtggaggc gcgagttgag gagcatgtcg cacaggaggt cgatggcgag
       61 gggcacatgt tcggcaagca ccctggcgtg atagcaggtt gcctccttat cggtgaaggc
      121 gttcaactgc ccgccgacgg tatctagcgt ctccgcgatg tcacgcgccg accgcgtcgc
      181 cgttcccttg aacagcatgt gttccagggc gtggcagatg ccagccagcg cgggcggttc
      241 atgcaccgac ccgacgccta cccagatccc gaccgtggcc gagaggacgt gcggaatctc
      301 ctcggtaaga acgcggatcc cgttgggtag caccgcgcag cgaactcgct cttccaacca
      361 gacgcccttc ccaagcggag cgccgaaaca gcgcgcccac gcagacataa caatgcgcgc
      421 ctcgcagcct gttgccagac cgcgaagacg cgcatcctgt agtgctacgg tttggggtca
      481 ggccccaaat cccctcgaat cagccccggc gctcctttgg ccggaacttg gcgctggggc
      541 gttcctcgcc accgggccgg ccaccgccgc caccgccgcg ttggccaccg ccacgctggc
      601 caccgccgcg ctgaccgccg ccacgctggc cgccactggg gcgctcgccc cgaggggcct
      661 cggccggggc agctgcctcg ccctcctccg gctccagaag gcccttgcgc gtcagcttga
      721 tcttgccctg cgggtcaatg tcgatgacct tgaccatcac ctcgtcaccg atcttgacca
      781 cgtcttcgac gcgggcaacc cggcgatgcg ccagctcgga gatgtggacc atcccctcgc
      841 ggccaggcaa gatctcgatg aaggcgccga acgactgaat ccccaccacc ttgccgaggt
      901 aagtctcgcc gacgcggacg tcgcgggtga tgtcgtcgat catcttgcgc gccatctcac
      961 cgccagcggc ctccaccgag gtgatgaaga cgcggccgtc ctgctcgata tcgatggtgg
     1021 cgccggtttc ggcggtgatc ttcttgatga tcttgccacc tgggccgatg acctcgccga
     1081 tcttgtccgg atggatctcc aggatgaaga tgcgcggcgc gtactcggag agttcctcgc
     1141 gtggcgcggg gagcacgtcg accatcttct ccaggatatg cattcggccg cgctttgcct
     1201 gcgccagggc gttcgccagc atctccatcg agaggccctc gatcttgatg tcgagctgaa
     1261 tcgccgtaat gccgtcgcgg gtgccggcga ccttgaaatc catgtcgccg gtgaaatctt
     1321 ccatcccctg gatatcggtg agaaccacgt actgatcacc gcgggtgacc aggcccatgg
     1381 cgacgccggc gaccggcttt cgcatcggca cgccggcatc catcagcgcg aggcagccgg
     1441 cgcacaccga gcccatggaa gaagagccgt tgctctccaa cacctcgcag actacgcgca
     1501 tggtgtaggg gaactcgcta tcgggcggaa gcatcggctg gagcgcgcgc tctgccaggg
     1561 caccgtggcc gatctcgcgc cggccggggc cacgcaacac gcgcgtctcg cccacgctgt
     1621 aaggcgggaa gttgtagtag agcatgaagt gcttctgctc cagcgccaac tccaggcctt
     1681 ccagcttctg accctcgcca ggcgatccca gggtggttgt ggcgagtacc tgtgtctgcc
     1741 ctcgggtgaa gagggcagag ccgtgggtac gcggcaggag ggagacttcg gcggtaatcg
     1801 gacgaacctc ttcgacgccg cggccatccg ggcgcttccc ttcgtccagg atcagggcgc
     1861 gaagctcttc cttgatcact ttatcgaccg cctcgctcag ctccgcttcg cgctcgggga
     1921 agtcgggggc cagctcggca acgatctcgc cgcgcaggag ctgaagtccg gcttcgcggg
     1981 cggccttgtc cggctcctga atcgcctcgc gaatgcgcgt tgcctcgcgc tcgcgcacgg
     2041 cgtcgatcag ctcgggatcg acgatgaagg aggggaaatc gcgtttcggc ttacccgcct
     2101 tggcggccag ctcctcttga gcggcgatga gggcgcgaat atgctcctgg gcgaactcta
     2161 acgccgcgag cagatcctcc tcagtcacct ctttggcgcc ggcctccatc atgacgacgc
     2221 tcgtcttggt ccctgcgacc accaggtcga ggtccccctc ctcagcctgt gcccacgagg
     2281 ggttgatgat gaactggcca tccactcggc ccacacgcac ggcggcaatc aggttaggga
     2341 aggggatatc gctgacggca agggcggcgg aggcgcctgt gacggcgagt acatcgggcg
     2401 ggtgatcctg atccacggag agtggagtcg ccacgatctg gacttcgttg cgcaggccgg
     2461 tggggaaaag ggggcgaatc gggcggtcga tcaggcggga ggtgaggacg gcaaactcgc
     2521 tcggccggcc ctcacgcttg atgcgcccgc cggggatccg gccaacggcg tacatgcgct
     2581 cttcgaagtc gcatgtcagg ggaaagaaat caacgccctc acggggttcc tggcttgcgg
     2641 tggccgtgac caggaccacc gtatcaccgg agcggatcaa gaccgctccg cctgcctgct
     2701 tggctacgcg accggtttca atgcttacgg tcgcctcacc gagtgttgct tccactacgg
     2761 taggcatcag tatttagctc tcctctgagt gcgcagttgc cacgcctgct cggagctcaa
     2821 cccgaggcaa ggtgcccggg agaggccacc gagggtctct cccgtccctg ctgcaagatt
     2881 ggctccgcaa aacgcgacct gtggcgaaca atgagcgggc gcgcgccgca acccgtctct
     2941 tagcgcctca gaccgagagc ggccaccaac gcccgatagc gctcgatatc tttattgctg
     3001 aggtagttga gcaggcgccg ccgatggccc accatcatca acaaaccacg ccgcgaatgg
     3061 tggtccttct tatgcgtgcg caggtgctcc gtgagctggt tgatgcgcgc cgtgagcagt
     3121 gcgacctgca cctccggcga gcccgtgtcc tgctcgtgct tcttgtgttg cgcaatgata
     3181 ccctgcttct gactaggctg caaaggcatg tccgtaaagt cctcctgctt gggaagggct
     3241 gctgcagcga gaaaccgctg ccggcgcgcg gcccgttcca cactccttct cttagttatt
     3301 ccgactcccg tcgccgccca ttgtagcata aagcagggcg gtgtgtcaaa gcctgcatcg
     3361 cgcggatgaa ggaaacgcgc agggacggtg gaaggacggc tgcccggcgt gggccggcat
     3421 cggcgcgcag gcccgcggcg ttcctccgcg cggcagggaa cccacacctc tcttccggtg
     3481 tcccaaataa ggtatcgtac agcacgcgca tggaggggca ggtttaggat cctgctagaa
     3541 aggggaaaac cactctaaga ttctccgttt cgacgcattg acagattccc ctgcctatga
     3601 tatgatgtta gttcgtgtgg tgtagctaac tggagacgga gggcatgcat gagcatagtt
     3661 gatgatttgg gcagggctcc ttccacagac gttgccacgg tcgtcgatat ccccaacctg
     3721 atcgagattc agaccgcatc gtatcactgg tttctcaagg acggcttgcg tgagttattc
     3781 cacaactttt cccccatcga ggatccgacg ggcacttacg cgctctacct ggatgattac
     3841 ctcctgaagg acgcgaagta caccattgag gagtgccgcg accgcgacat tacctacgag
     3901 cgccccatca gcgcgcgcat ccgcctcctg aacaagcgta ccggcgagat catggaggat
     3961 gaggtctacc tgggcgatct gccggtgatg accgagaagg gcacctttgt cattaacggc
     4021 gccgagcgtg tcgtcgtcag ccagttggcc cgttcgcctg gcgtctattt ccgcgataac
     4081 atcgactact ccgggcgcgt gctcttctcg gcccaggtga tccctgcgga gggggcctgg
     4141 atcgaggtgg agaccgatgc caacgacgtg atcttcgtgc gcatcgggca ggcgcgcaag
     4201 ttcccgatca ccacactgct gcgcgcgctg aacgctttcg accatgagct cggcgagacc
     4261 cagcgcccgc aaacggcgac cacctatcag atttatcggc tctttggcac cgttcgggtg
     4321 gtcgaaaacc ccaccgtcga gaacctgatc tccttcgatg ccggcccggt gatccgcatc
     4381 tatcaggacg tgacggatcc caactccgga aaggtcctcg ccaaggcgtt cgataagctg
     4441 gatcgccccc tggctgagaa gatcgaggcg gccggaatcc agaatatcga gattattgag
     4501 atcccgcgct acatcgaggc gaccatcgag caagacgccg tcaccgagaa ggaaaacccc
     4561 cagcgggccg aggatgaggc gctgctcgat atctaccgaa aggttcgccc cggtgatccg
     4621 gcgactcgcg agagcgcgcg cgctctcctc agcagcatct tctttgacat caagcgctac
     4681 gaccttgccc gggtcgggcg ctataagatg aataagaagc tgggcattga cctcccgctc
     4741 actgttcgaa gcctgaccaa ggacgacctg gttgccatcg tccgctatat cattaagctg
     4801 agccacaatg aaggctccgt cgatgatatc gaccacctgg agaacaagcg cgtccggtcg
     4861 gtcggcgagc tgctgcagag ccagttccgc ctgggcctcc tgcgcatgga gaaggtagcc
     4921 aaggagcgca tgacctccct ggagccggac acggtctcgc cccaggcggt tgtctccgtg
     4981 aagccgatct cggccgccat caaaagcttc ttcgggagca gccagctctc ccagtttatg
     5041 gatcagacga acccgctggc tgagcttacg cacaagcgcc gcctgagcgc gcttggcccg
     5101 ggcgggcttt cacggcagag cgccaagctg gaggtgcgcg acgtccacca cagccactac
     5161 gggcgcatct gccccatcga gacgccagaa ggcccgaaca tcggtctgat cggctcgatg
     5221 gcgatgcacg cacgcatcaa tgagttcggg ttcctggaga ccccgtaccg caaagtcgtc
     5281 gatgggaagg tgacggacca gatcgactat ctgaccgccg atgaggaggc gccctaccac
     5341 atcgc
//
LOCUS       DAOSCV010000191         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9733_length_5333_cov_3.401857, whole genome shotgun sequence.
ACCESSION   DAOSCV010000191 DAOSCV010000000
VERSION     DAOSCV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9733_length_5333_cov_3.401857"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLY56_06090"
     CDS             complement(<1..389)
                     /locus_tag="PLY56_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ21084.1"
                     /translation="MSDVISLGILVADVVAKPIKQWPEEGQLILVDQFELHTGGCAMN
                     TAVALSRLGLSAGIIGKVGQDAFGDYLVAALQREGVDTAGIRRQEGVNTSASMVCVTP
                     SGERSFIHYLGANAELGAEDVDFDIVER"
     gene            complement(422..1438)
                     /locus_tag="PLY56_06095"
     CDS             complement(422..1438)
                     /locus_tag="PLY56_06095"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOJ21085.1"
                     /translation="MKAVVIDEPRSVRLADLPMPHAGADEVVIRVRACGVCGTDLHIF
                     HGGFRADYPVIPGHEFSGEVVEAGAAVEHVRVGDHVTIDPNISCGVCPFCRRGEIHLC
                     THLTALGVNRPGGFQEFCLAPAKQVYQLPHDLPWEAGAFVEPLACCVHGIDRAQIRAS
                     DTVVILGAGSIGLLITQLVRLAGASRVIVSEPSEEKAELARALGADTVLNPRSTDLTQ
                     AVRDLTGIGADLVIECVGHPGTVAQALTLPRRGGRVLLFGVNPKEAEVPIRPYEVFLN
                     ELTIIGSYINPCTHARAIDLVASGRVKVDPLVSHRLPLDDFPRAMEIAEAGGAVKIMV
                     AGRS"
     gene            complement(1488..3668)
                     /locus_tag="PLY56_06100"
     CDS             complement(1488..3668)
                     /locus_tag="PLY56_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21086.1"
                     /translation="MRRHRSLSYAILLAVAAATALLPLVTPALAAEKAEPAQPVIASP
                     PGAAGTYQDRDGVPHRWHVNEAFALLWDGAPYLPAGMVFQPRSLAEPEKAEAWEADSA
                     TLHAWKEAGIRDVLIQPDRPATAIPVAAWQRLIDQLETLRLCYGIALPIAPIQPAAGY
                     YVRTGAFRLGPLKAAGEHATEIAIPDVKDLRAQRIAAATIGVDSGALLRVDWAKASPA
                     AGGLRATVNLDKAPREEAIIELRPLLASTPGLPDLWSHFGAVRDSLIALAPLKFGSGL
                     RFFVHPLAGLIDLDGSAGYVVPTSEAYRLEFEAFLARKYRKVEALRSQWDFRGELPSD
                     LQTAARLVPLAISSARGRTTGYLLDEKESRCYAIDPGDSRFWYDHLYFREHSLREQAN
                     QLAQFIGEQLADVPVVMECAGSIRYFHINNQPSGGFAGIGVRAAPEHVAAKAATAIGA
                     ARLAASRPWCLALALEACDTAARLQGAVDTVRQAGAKGWFVAPPSTAPAELPAWVVAQ
                     RAAQESRGDAAGYTPQYILFPHSIRETGGFGHQQQPVGADPGPVREDCWWIPTLAGWD
                     PIDLGPDLRGFGIRDQSGYRILMWSPKGKRRIHLLPRTYETVEVRNLDGKVIARHKKG
                     KRLRIDLSEQPVIITQLNPLQATPIELAQAELEELERLTRELGLKGHSVRAFQGAITL
                     ARTLKPEVDPMGVRQLIRAPLATARRLLEAPETPETSPTMHSLE"
     gene            complement(3766..4458)
                     /locus_tag="PLY56_06105"
     CDS             complement(3766..4458)
                     /locus_tag="PLY56_06105"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ21087.1"
                     /translation="MARGWRVPLLSVILAMAAEAAWAGPAHVVKRDANALRFKAVYMR
                     EWVHVRKVPADIVVNANFYWRGAPLGILVKEGRLVNGGLKQKPPRTSFVVQQVNGRQR
                     VRILEVYEKGGRLYTSSGPLTNVRTLVQAGPRLVTNGRVAVARSREGFRNDVARRTRH
                     VGLGLTRDGKLLIVAQSDVTLTEFARTFVRLGAQDAMNLDGGSSATLAVNGKVRMGSG
                     RILAGLAINSPK"
     gene            4793..4987
                     /locus_tag="PLY56_06110"
     CDS             4793..4987
                     /locus_tag="PLY56_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21088.1"
                     /translation="MHPPQHPLHGGAAAGETEWYPRVIGKPADSDEGAHAASIQEWTM
                     LQIQGNRASAPGNRSRGEPL"
BASE COUNT          927 a         1739 c         1797 g          870 t
ORIGIN      
        1 cgctcgacga tatcgaaatc tacgtcttcg gcacccagct ctgcgttggc ccccaggtag
       61 tggatgaaag agcgctcgcc actcggagtg acgcagacca tggaagccga ggtgttcacg
      121 ccctcttgcc ggcgtattcc agcggtatcc actccctcgc gctgcaacgc ggcgacgaga
      181 tagtcaccga aggcgtcttg gcccaccttg ccgatgatgc ccgcagagag cccgaggcgc
      241 gatagcgcca cggcagtgtt catcgcgcac ccgccggtgt gcagctcaaa ctggtcgacc
      301 agaatcagct gcccttcttc aggccattgc ttgatgggtt tggccaccac gtcggcgacc
      361 aggatgccca gcgatatgac gtcgctcacg ccagttacct cccttggcgg ccgaccgggc
      421 gctaggaccg accggctacc atgatcttga cggctccacc cgcttcagca atctccatag
      481 cgcgcgggaa atcgtcgagc ggaagccggt gcgagaccag cggatctacc ttcactctgc
      541 cgctggcgac caggtcgata gcgcgtgcgt gcgtgcaggg attgatgtag gatccgatga
      601 tcgtcagttc gttcaggaac acctcgtagg gccggatggg cacttcggcc tccttcgggt
      661 tgaccccgaa gagaagcacc cgcccgccgc gccgggggag tgtcaacgcc tgggccacag
      721 tgcccggatg gccgacacac tcgatcacca gatccgcgcc gattcccgtc agatcgcgca
      781 cggcctgtgt cagatccgtc gatctggggt tgagcacggt gtctgctccc aatgcgcgtg
      841 ccaactccgc cttctcctcc gagggttcag agacgatcac ccgcgacgcc cctgctaacc
      901 ggaccagctg ggtgatcagc aggccaatcg agcctgcacc caggatgacg accgtatccg
      961 aagcccggat ctgcgcgcga tcgatcccat gaacgcagca agccagcggc tccacgaacg
     1021 cccctgcctc ccaggggagg tcgtggggca actggtatac ctgcttcgcg ggcgccaggc
     1081 agaactcctg gaaaccaccc gggcgattta cccccaaagc cgtgagatgg gtgcacaggt
     1141 ggatctcgcc gcgtcggcag aaggggcaga cgccgcatga gatgtttgga tcaatggtga
     1201 cgtggtcgcc tacgcgcacg tgctcgaccg cggcgccggc ctccactacc tcgccactga
     1261 attcatgacc agggatcacc ggatagtcgg cacggaatcc accgtgaaag atatgaagat
     1321 cggtgccgca gacgccgcac gcccgcacgc ggattacgac ttcatcggct ccggcatggg
     1381 gcatgggcag gtctgcgagg cgcaccgaac gcggttcatc aatgacaaca gctttcatta
     1441 agccctccac ccctcatgct accatagaga gtggcgcggt cacaaggtta ctccagacta
     1501 tgcattgtcg gagacgtctc gggtgtttcg ggggcctcca ggaggcggcg cgcggtggcg
     1561 aggggcgcgc ggatgagctg gcgaacgccc atggggtcca cctccggctt cagggtgcgc
     1621 gcgagcgtga tggcgccttg gaacgcgcgc acgctgtgcc ccttcaagcc gagttctcgc
     1681 gtcaatcgct ccaactcttc gagctcagcc tgggccagct caatcggcgt ggcctgcagc
     1741 gggttaagct gggtgatgat caccggttgc tcgctgagat cgatgcggag gcgcttgccc
     1801 ttcttgtgcc gggcgatcac tttgccatcg aggttgcgca cctcaacggt ctcgtaggtg
     1861 cgcggcagga ggtggatgcg gcgcttccct ttgggcgacc acatcaggat gcggtagccg
     1921 gattggtcgc ggatcccgaa gccgcgaagg tccggcccga ggtcgatcgg gtcccacccg
     1981 gccagggttg ggatccacca gcagtcttcc cgcaccgggc cgggatccgc gccgactggc
     2041 tgctgctgat gcccgaagcc ccctgtttct cggatgctat gcgggaagag gatgtactga
     2101 ggagtgtagc cggcagcgtc gccacggctt tcctgggcgg cgcgctgggc cacaacccac
     2161 gccggcagct ccgcaggtgc cgtggacggt ggcgcgacaa accatccctt cgcgccggcc
     2221 tggcgcacgg tgtctacggc cccctgaagc cgggcagccg tgtcgcatgc ctccagggcg
     2281 agcgccaggc accatggccg gcttgccgcc aggcgcgccg cgccgatggc ggtggcggct
     2341 ttagcggcca cgtgctctgg ggcggcgcgc accccgatac cggcgaaccc gccagacggc
     2401 tggttgttga tgtggaagta gcggatgctg cctgcgcact ccatcaccac cggcacatcg
     2461 gcgagctgct cgccgatgaa ctgggcgagt tggttggcct gctcgcgcag cgagtgctcg
     2521 cggaagtaaa ggtgatcata ccagaagcgt gagtcgccgg gatcgatggc gtagcagcgc
     2581 gactccttct catccagcag gtagcccgtc gtccgtcctc gagccgatga gatggccagg
     2641 ggaacaagcc gcgcggcggt ctgtagatcg ctcggaagct cgccgcggaa gtcccactga
     2701 gagcgcaacg cctcgacctt gcggtacttg cgcgcgagaa acgcctcgaa ctccaggcgg
     2761 taggcctctg aggtgggcac gacgtaaccc gccgacccgt cgaggtcgat gaggccggcc
     2821 agtggatgca caaagaaacg aagtccgctc ccgaacttga gtggcgccag ggcgatcagc
     2881 gagtcgcgga ccgcgccaaa gtggctccac aggtcgggca ggcccggcgt gctggcgagg
     2941 agcgggcgca gctcgatgat cgcctcctcg cgaggcgcct tatcgaggtt caccgtagcc
     3001 cgcaggccac ccgccgccgg agacgccttc gcccagtcca cgcgcagcag cgccccggaa
     3061 tcgacgccga tggtcgcggc ggcgatccgc tgggcgcgaa ggtccttgac gtcggggatg
     3121 gcgatctccg tcgcatgctc gcccgccgcc ttcaggggcc ccaggcggaa tgccccggtg
     3181 cgcacgtagt agcctgccgc cggctggata ggagcgatgg gcagggcgat cccatagcac
     3241 agcctcaacg tctcaagctg atcgatcagg cgctgccagg cggcgaccgg tatcgcggtg
     3301 gcgggccggt caggctggat cagcacgtca cggatgccgg cctctttcca cgcgtgcagc
     3361 gtggcggagt cggcttccca cgcctctgcc ttctcgggct ctgccaggga gcgtggctgg
     3421 aacaccatcc cagcaggaag atagggggcg ccgtcccaca ggagcgcgaa cgcttcgttg
     3481 acgtgccacc ggtgcgggac gccatcgcga tcctggtagg tgccggctgc tccgggggga
     3541 gaggcgatca ctggctgggc gggctctgct ttctctgccg ccagggccgg cgtgaccagg
     3601 ggaaggagcg cggtggccgc cgcgaccgcg aggagaatag cgtatgagag agagcggtgg
     3661 cgtcgcattc tcgtgccagg ttcctttcca gggcgatggg gtgacgaagg cattgtatca
     3721 taggggtagg ggggagtcaa tgaacgctgc gcgcctcgac gggcgctatt tgggggagtt
     3781 gatcgccaat ccggcaagga tgcgccccga gcccatgcgc actttcccgt tgacggcgag
     3841 cgtggccgag ctcccgccat ccaggttcat cgcatcctgc gcgcccaggc gtacgaaggt
     3901 acgcgcgaac tcggtcaggg tgacatcgga ttgcgccacg atgaggagct tcccgtcgcg
     3961 agtgagcccg aggcccacgt gtcgcgtccg ccgggccaca tcgttccgga aaccctcccg
     4021 cgatcgcgcg acggcgaccc ggccattcgt cacgaggcgc ggcccagctt gtacgagcgt
     4081 gcgcacattc gtgagtggac ccgaactcgt gtagagccgg ccccccttct cgtacacctc
     4141 cagaatgcgc acgcgctgcc ggccattcac ctgctgcacg acgaaggatg tgcgcggcgg
     4201 cttctgcttc agcccgccgt tgacgagccg gccctctttc acaagaatgc ccaggggagc
     4261 gcctctccag tagaagttgg cgttgacgac gatatcggcg ggcaccttgc gcacatgcac
     4321 ccactcacgc atgtacacgg ctttgaagcg tagcgcgttg gcgtcgcgct tgactacgtg
     4381 agcggggccg gcccaagccg cctccgctgc catcgccagg atcaccgaga gcagcggcac
     4441 ccgccagccc ctcgccatgc cggatctccc tatgccgcgt ccgccatcca ccatcaccct
     4501 gtgccgtcac accgggagga tgcatggagg gtgccagtca ccggagcctc atttggagag
     4561 aacgagcagg ggatggtgcc acgccgcatt gcacggacgc gcgtgagcga gagtgtcgga
     4621 ccgcgtggtg ccagagagag cccgcgagtt ccctcctggc ggcgcctcag gcggtaaaag
     4681 cgccctacag ggtcggaggt ccggacgcgg agcctacatc gaggagagca tccgtgggtt
     4741 tggggtggga ttcgctggag gccgcggcgg gcaagtcaaa gagggtgtgg agatgcatcc
     4801 gccgcagcat ccgctccacg ggggcgctgc agcgggagag acggagtggt atccccgcgt
     4861 gatcggcaag ccggcggatt cggatgaggg cgcacacgcc gcgagcatcc aggagtggac
     4921 aatgctccag atccagggca atcgcgcgag cgcccctggc aatcgctccc gcggcgagcc
     4981 gctctaacgc agcactcgtc tgcgcatcga gggcgcaccg cgcctgcacg actgactcgt
     5041 caccggttcg ctcaacagag agctcagttt tggctggcca catgcctctt accccttcct
     5101 gagcgggcga tgcgaggcgc gcggatgcaa catcgaggcc aaacgaaaga ggcagcgcgg
     5161 gctagcgccc gggatccgta atgccgagat cgacccctgt ggttttcaag tcgcgcacga
     5221 ggcggttgaa gcgctctgtg aggcgggaat aacgggcgtc caggatggcg acgcgtgatt
     5281 gaatctgttg ggcatccggg gcagagacgg cggcgccttc ggagtcggtc acc
//
LOCUS       DAOSCV010000192         5332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9735_length_5332_cov_3.517718, whole genome shotgun sequence.
ACCESSION   DAOSCV010000192 DAOSCV010000000
VERSION     DAOSCV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9735_length_5332_cov_3.517718"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..1998)
                     /locus_tag="PLY56_06115"
     CDS             complement(475..1998)
                     /locus_tag="PLY56_06115"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ21089.1"
                     /translation="MRRIAIFDTTLRDGEQSPGASMNLSEKLDVARQLARLGVDVIEA
                     GFPISSQGDFDAVRLVAEKVTGPTIAGLCRVVPADIDRAGEALRPAAKRRIHTFIATS
                     DIHLKHKLKKSREEVLEMAVAGVKRARNFTDDVEFSAEDASRTDPDYLCQVVEAVIDA
                     GATTVNIPDTVGYAVPHEFARLIATLRERVPNIDRCVLSVHCHNDLGLAVANSLAAIM
                     AGAGQVECTINGIGERAGNAALEEVVMAINTRADQFQVSTQINTREIYRTSRLVTDVT
                     GIQVQPNKAVVGANAFAHEAGIHQHGVLQERTTYEIMSAQDVGIAESRLVLGKHSGRH
                     AFEKRLHELGYTLSKEDLDRAFARFKELADKKKEIFDADLESIVADEVHSVPQEYSLE
                     YMTVVAGLEGYPTATIKVRRGQSELVDAGLGVGTIDAVYKTIDKLLGIPHELVEYSVK
                     SVTGGTDALAEVTVKVGADGRTYAGRGASLDVVEASAKAYMQALNKLVHYRATSAQR"
     assembly_gap    2139..2148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2149..3125)
                     /gene="ilvC"
                     /locus_tag="PLY56_06120"
     CDS             complement(<2149..3125)
                     /gene="ilvC"
                     /locus_tag="PLY56_06120"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007304727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOJ21090.1"
                     /translation="MAKVYYDADVNLDLLKGKTVAVIGFGSQGHAHALNLADSGVQVV
                     VGAVPGGRGWAAAEKAGAKVMTTPEAAAAGDVIMILTPDETQPAIYEEQIRPHLKPGN
                     ALAFAHGFNIHFGQIVPPKDVDVIMIAPKGPGHLVRRQYQEGKAVPALIAVYQDATGH
                     AKDLALAYAKGIGSTRVGVLETTFKEETETDLFGEQAVLCGGASALIMAGWETLVEAG
                     YQPEIAYFECLHELKLIVDLIYEAGIAGMRYSISNTAEYGDVTRGPRVITEETRAEMR
                     RILGEIQRGEFAREWILECKAGKPVYRALTRMGAEHPIDLALLFDEARA"
     gene            complement(3277..3630)
                     /locus_tag="PLY56_06125"
     CDS             complement(3277..3630)
                     /locus_tag="PLY56_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21091.1"
                     /translation="MRELIDEQTANAVMNTLAVALPLAGAVIGALAGTLARRPVPGAL
                     RGLAVGCLGILNWVLWRLYNAIMDQLGLDTVKNLLVNLALFVALGIAGGVAAGLYLRS
                     RQPAPDEETPPAQQA"
     gene            complement(3656..4141)
                     /gene="ilvN"
                     /locus_tag="PLY56_06130"
     CDS             complement(3656..4141)
                     /gene="ilvN"
                     /locus_tag="PLY56_06130"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ21092.1"
                     /translation="MKHTLSVLVENRFGVLARVAGLFARRGYNIETLAVAPTDDPSIS
                     RMTIVVAGDDSDLEQITKQLNKLIDVIKVNDYTDVATVERELALIKITAEPGVRGEIM
                     QIADVFRANVVDVGERSFILEVTGKVDKIDALTRLLEPYGIREIVRTGKIVLSRGIQP
                     L"
     gene            complement(4397..>5332)
                     /locus_tag="PLY56_06135"
     CDS             complement(4397..>5332)
                     /locus_tag="PLY56_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ21093.1"
                     /translation="ETHPLSLGMLGMHGTAYANYAVHHCDLLVAIGARFDDRVTGKLQ
                     TFAPEAKIIHIDIDPAEIGKAKPADVPIVGDAKGILEAILAKVEPSDHSEWLHQIRQW
                     KEEFPLQYREDGTLKPQQILQELHAVTGGRAIVSTDVGQHQMWAAQYYLCDRPRQFLA
                     SGGLGTMGFGLPAAMGAQFGRPDETVVCVCGDGGFQMTLQEMIVAVEHRLPVKIILLN
                     NGYLGMVRQWQQMFWQNRLSGVDISMQPDFVKLAEAYGAAGREITEARDVRPALEWAM
                     EIRDRPCLLNFRVAREENVFPMIPAGGSIDQMLID"
BASE COUNT          895 a         1770 c         1687 g          970 t
ORIGIN      
        1 gcagagagcg tgtcaggcgc gagcctgcca gttagggggt gttgttgggg atcaagccgc
       61 ggcgcggctc caaccgatgg actgccggtg gcgtatgtcc cggacgtgcg tggacagcca
      121 ccggcaggta gtctccttct ccgtatgagc gccggcggtg aaccggcgcg atgaaaactc
      181 tcttttcaca attgccatta taccacacgc ttgcacgagc tgcaaggcga tagccgcggg
      241 tctgaagtcg cgcgagttgc gcccgcgcga tgcgcggagg cgagcacgtt acgcaggagc
      301 gcggcctccc ccggcgcgcg aagccagaag ctccgcgctg cttctcagca aagccccttc
      361 gggcctgttc agcccagccc ggcagggctt cgcacgcaag cagcgcggag gttaaacgcc
      421 gcgcgacccc ccggcgtgcc gtttgctccc gcgtgaccga tgcaatgcgg gtgtttatcg
      481 ttgcgcggat gttgcgcggt agtggacgag cttattgagc gcctgcatgt aggccttggc
      541 gcttgcctcc accacatcga ggctcgcacc gcgaccggcg taggtgcgcc catcggcgcc
      601 caccttcacg gtcacctcgg caagcgcgtc tgtgccgccc gtcaccgatt tcaccgagta
      661 ttccaccaac tcgtgcggga tgccgaggag cttgtcgatc gtcttgtaca ccgcgtcgat
      721 ggtgcccacg ccgaggccgg cgtccaccag ctccgattgc ccgcggcgca ccttgatggt
      781 cgccgtggga taaccctcca gaccggccac caccgtcatg tactccagcg aatactcttg
      841 gggtaccgag tggacctcgt cggcgacaat cgactcgagg tcggcgtcga agatctcctt
      901 cttcttatcc gccagctcct tgaaacgggc gaaagcgcgg tcgagatcct ccttgctgag
      961 cgtgtagcca agctcatgga ggcgcttctc gaaggcatgc cgtccggagt gcttgcccag
     1021 gaccaggcgg ctttcggcga tgccgacatc ttgcgcgctc atgatctcgt aggtggtgcg
     1081 ctcctgcagc acgccgtgct ggtgaatgcc tgcttcgtgt gcaaaggcgt tggcgccgac
     1141 gactgccttg ttcggctgga cctggatccc ggtcacgtcg gtgaccaagc ggctggtgcg
     1201 gtagatctcg cgcgtgttga tctgggtgga aacctggaac tggtcggcgc gcgtgttgat
     1261 cgccatcacc acctcttcga gggcggcgtt gccggcgcgc tcgccgatgc cgttgatggt
     1321 gcactccacc tgcccggcgc cggccatgat cgccgccagc gagttggcga cggccagccc
     1381 gaggtcgttg tggcaatgga cgctaagcac gcaccggtca atattcggca cgcgctcgcg
     1441 aagcgtggcg atcagccggg caaactcgtg gggcaccgcg tagccaacag tatccgggat
     1501 attgaccgtg gtggcgccgg cgtcaatgac cgcctcgacc acctggcaga ggtagtctgg
     1561 atcggtgcgc gaggcatcct cggcagagaa ctccacgtca tccgtgaagt tgcgcgcccg
     1621 cttcacgccg gccacggcca tctccaggac ttcctcgcgc gacttcttca gcttgtgctt
     1681 cagatggata tcggacgtgg cgatgaaggt atggatacgt cgcttcgcgg ccgggcggag
     1741 ggcttcccct gcgcggtcaa tgtccgcagg gacaacacga cagagcccgg caatggttgg
     1801 cccggtgacc ttttccgcga cgaggcgtac cgcgtcgaaa tctccttgag agctaatggg
     1861 gaaccccgcc tcaatgacat caacgcccag gcgtgccaac tggcgtgcca catccagctt
     1921 ctcgctcagg ttcatgctgg cgccaggcga ttgctcgccg tctctcaaag tggtgtcaaa
     1981 gattgcgatg cggcgcatgg gattcacccc ttcagatatt ggagtggcgc gcgcgctggc
     2041 gcgcgccact ccgatgggtt tacgagacgt ccttcaggtc tttcttctgc aggtagggca
     2101 tcatcgcgcg aagccggcgg cccacctctt cgatcgggnn nnnnnnnntg cgcgcgggcc
     2161 tcatcgaaga gcagggcgag gtcgatcggg tgctcggcgc ccatgcgcgt gagcgcgcga
     2221 tacaccggct tgccggcctt gcactcgagg atccactcgc gagcgaactc gccccgctga
     2281 atctcgccca ggatgcggcg catctcggcg cgggtctcct cggtgatcac gcgcgggccg
     2341 cgcgtcacat cgccatactc tgccgtgttg ctgatcgagt agcgcatccc ggcgatgccc
     2401 gcctcgtaga tcagatcgac gatcagcttc agctcgtgca ggcactcgaa gtaggcgatc
     2461 tccggctggt agccggcctc gaccagcgtc tcccacccgg ccatgatgag ggcggaagca
     2521 ccaccgcaaa gcactgcctg ctcgccgaac agatccgtct ccgtctcctc tttgaaggtg
     2581 gtctcaagca cgccgacgcg cgtggagccg atccccttgg cgtaggcgag cgcgagatcc
     2641 ttggcgtggc cggtggcgtc ctgatagacg gcgatgagcg ccggcacggc tttcccctcc
     2701 tggtactggc ggcgcaccag gtgcccgggg cccttcgggg cgatcataat cacatccacg
     2761 tccttggggg gcacgatctg gccaaagtgg atgttgaagc catgcgcgaa cgcgagggcg
     2821 ttccccggct tcagatgggg gcggatctgc tcttcataga tggccggctg cgtttcgtcc
     2881 ggcgtcagaa tcatgatcac atcgccggcc gcggctgcct cgggggtggt catcaccttg
     2941 gcgcccgcct tctccgcggc agcccaaccg cggccgccgg ggaccgcgcc aacaacaact
     3001 tgcacgccgc tgtccgccag gttgagcgca tgcgcgtgac cctggctgcc gaacccgatc
     3061 acggccaccg tcttgccctt gagcaagtcc agattcacgt ccgcgtcata atagacctta
     3121 gccaatgtcc ttctccttcc gcgcgcctgg ggcgcgcgcg tccaggtgtt ggggaatctg
     3181 gagccgggcg ccagtatggc acctttcctc atgcttgggg ggagagcaga cgcgtgccct
     3241 cccagatccc cgcacctgga tgcaactcac ccgctgttac gcctgctgcg ccgggggcgt
     3301 ctcctcatcc ggggccggtt gcctcgaacg cagataaagc cctgccgcca cgccgccagc
     3361 gatcccgagc gcgacgaaga gggccaggtt gaccagcagg ttcttgaccg tatctagacc
     3421 aagttggtcc ataatggcgt tataaagacg ccacaagacc cagttcagga tgccgaggca
     3481 gcccacggcc aggccgcgca gggcacccgg gaccggccgg cgcgcgagcg tgccggcgag
     3541 ggcgccgatg accgcgccgg ccagtggtag ggccaccgcc agcgtgttca tcaccgcgtt
     3601 cgccgtctgt tcgtcaatca gctcgcgcat ctccatggcc ggcaccccgc gtcactcaca
     3661 gcggctggat gccgcgcgag agcacaatct tgcccgtacg gacgatctcg cggatgccat
     3721 agggctccag cagccgggtc agcgcgtcaa tcttgtccac cttgccggtc acctcaagaa
     3781 tgaagctgcg ctcgcccaca tccaccacgt tggcgcggaa aacgtcggcg atctgcatga
     3841 tctcgccgcg cacgcccggc tctgccgtga tcttgatcag cgccaattcg cgctcgacgg
     3901 tggcgacatc cgtatagtca ttcaccttga tcacgtcgat cagcttgttg agctgcttcg
     3961 tgatctgctc aaggtccgaa tcatccccgg cgacgacgat cgtcatgcgc gagatggatg
     4021 gatcgtctgt cggggccacc gcgagcgtct cgatattgta gccgcggcgg gcaaacaagc
     4081 cggctacccg ggccagcacg ccgaaacggt tctccaccag aaccgacaga gtatgtttca
     4141 cagaattagt ggcgtcgaaa gcccacatgc cgcagcggtg ggcggaagac accagtcgcc
     4201 cgccgagggc gacccctcct ttctttcgag ttttgcaaac cccatgagtg tggcacagcg
     4261 atctcgcgca aggcaccccc tcggggagat aacgctcggt gtgccgcacc ttgaaggcct
     4321 gggagtggcc ggctcgcgca cagaagcgcc cgcgcggcaa gccacgccgg cgcgcgcgcc
     4381 caagcccgcc tctctcctaa tctatgagca tctggtcgat ggacccccct gcggggatca
     4441 tcgggaaaac gttctcctcg cgtgccacgc ggaaattgag caggcagggg cggtcgcgga
     4501 tctccatcgc ccattccagt gccgggcgca cgtcgcgcgc ctcggtgatc tccctgccgg
     4561 cggcgccata tgcctcagcg agcttcacga aatccggctg catggagata tccaccccgg
     4621 agaggcggtt ctgccagaac atctgctgcc actgccgcac cataccgagg tagccgttgt
     4681 tgagtaggat gatcttcacc gggaggcgat gctccacggc gacgatcatc tcctggagcg
     4741 tcatctggaa gccgccatcg ccacagacgc acaccacggt ctcgtcgggt cggccgaact
     4801 gcgcgcccat ggccgcgggc aggccgaagc ccatcgtgcc caacccgccc gaggcgagga
     4861 attggcgcgg ccggtcgcac aggtagtatt gcgcggccca catctgatgc tggcccacgt
     4921 cggtagagac gatcgcccgg ccgccggtca ccgcatgcag ctcttggaga atctgctgcg
     4981 gcttcaacgt gccatcctcg cgatactgga gcgggaactc ctctttccac tggcggatct
     5041 ggtgaagcca ctccgagtgg tcggaaggct ccaccttggc caggatggcc tcgaggatgc
     5101 ctttggcatc gccaacgatc ggcacatccg ccggcttggc cttgccgatc tctgccgggt
     5161 cgatgtcaat atggatgatc ttggcctcgg gggcgaacgt ctggagcttt ccggtcacgc
     5221 ggtcatcaaa gcgcgcgccg atggcgacga ggagatcgca gtggtgcacc gcgtagttgg
     5281 cgtaggccgt gccatgcatc cccagcattc ccagcgagag ggggtgggtc tc
//
LOCUS       DAOSCV010000193         5316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9772_length_5316_cov_3.066907, whole genome shotgun sequence.
ACCESSION   DAOSCV010000193 DAOSCV010000000
VERSION     DAOSCV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9772_length_5316_cov_3.066907"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /gene="acnA"
                     /locus_tag="PLY56_06140"
     CDS             complement(<1..1557)
                     /gene="acnA"
                     /locus_tag="PLY56_06140"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HOJ21094.1"
                     /translation="MPDSFGSRSSLRVDGREYQIYRLDALERAGFSLARLPYSLKILL
                     ENLLRTEDGVSVTAQEIEALARWDPAAEPSREIAFTPSRVLLQDFTGVPAVVDLAAMR
                     DAMAALGGDPRRINPLQPVELVIDHSVQVDAFGTPDAFRINARHEFERNRERYEFLRW
                     GQKAFDNFRVVPPDTGIVHQVNLEYLARVVFATEKGGIVTAYPDTLVGTDSHTTMING
                     LGVLGWGVGGIEAEAAMLGQPISMLIPQVVGFELKGALPEGATATDLVLTVTQMLRAK
                     GVVGKFVEFYGEGLASLSLADRAVLANMAPEYGATCGIFPVDAETIRFLKMSARPEHQ
                     VRLVEAYMKEQGLFHDADTPVAAYSDTLSLDLGSVEASIAGPRRPQDRVPLREAKRQF
                     AAALPALLEAAKGSASDINAQATCTLDGKEHTLGHGAVVIASITSCTNTSNPSVMIAA
                     GLLAKKAVERGLKSQPWVKTSLGPGSKVVTDYLADAGLLPYLEQLGFHLVGYGCLTCI
                     GNIGPLPEPVR"
     gene            complement(2009..3121)
                     /locus_tag="PLY56_06145"
     CDS             complement(2009..3121)
                     /locus_tag="PLY56_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ21095.1"
                     /translation="MTDRRAEIESYMKFVVDRQGSDLILKSGRRPVARVFGDMTDVTG
                     TPVMHDELVRELVFSLLTDQQREQFLREYELDFAHEIPGLARFRVNLMFQRGTVTMVA
                     RVIPFTIKTAKELGLPPVCLEFCMRPRGLVLVTGPTGSGKTTTLAAMVHHINQNRPVH
                     ILTIEDPVEFVHTPIKALINQRELGRDTHSFANALREVLRQDPDVILVGEMRDLETIS
                     LAVTAAETGHLVLATLHTTDAVQTVDRLIDVFPPHQQAQIRMQISVNLVGIISQTLVK
                     RADGSGRVAAYETMKATPAISNLIREGKTHQIGSMVQTGLKQGMISLDQYLVYLVRNK
                     VVSYQAALDKSQNPSEFETMYKEAEAEKAAAAAKQR"
     gene            complement(3230..4381)
                     /locus_tag="PLY56_06150"
     CDS             complement(3230..4381)
                     /locus_tag="PLY56_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOJ21096.1"
                     /translation="MALNYTMDDLLRALVEHGGSDLHIRVGLPPLVRIRGDLTPLNYP
                     PLTPEDAEELMFSLMNEERKRKFLETNELDMAYSVKDVARFRVNALRQQGYVGGVMRC
                     IPYEIKTLEQLGLPPVIKDIVLRPRGLVLVTGPTGSGKTTTLAAMIRYINETTAKHII
                     TIEDPIEFVHEDIKSIVQQREVHEDTHSFQNALKHVLRQAPDVILVGELRDLETIHLA
                     ITAAETGHLVFGTLHTTDAAQTVDRAIDVFPPDQQAQIRMQLSVTILAIICQTLLPTA
                     DRQSRIAAFEIMIATPAIRSLIREGKTHQLPTEIQQGGELGMQALDGHLVQLLVSGKV
                     TYEEALLKCGNPKEFEMRVEKAFSQRGLPLPTQMTSQAPASGQAGQQRK"
     gene            complement(4635..>5316)
                     /locus_tag="PLY56_06155"
     CDS             complement(4635..>5316)
                     /locus_tag="PLY56_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ21097.1"
                     /translation="SSTLASMVDLINSTKKVHIITVEDPIEFVHPDKVGIVSQREVGV
                     DTENFQDALKYMMRQSPDVIMVGEMRDLETFTVALQAAETGHLVFSTLHTASAPETLD
                     RIVNMFPPHEKPMISQRLSNSLRGVISQKLVPRMDGSGRMAAVEVMVVTPTVAKLIEE
                     GRFNATYPVIAEGEHWGMQTMNQCLLRYYTSGLISADEALFAAGNQTELRQMMRQALE
                     SARASQRG"
BASE COUNT          890 a         1652 c         1800 g          974 t
ORIGIN      
        1 gcgcaccggc tccggcagcg gaccgatgtt gccgatgcag gtcaggcagc catagcccac
       61 caggtggaac ccgagttgct ccaggtaggg gaggagcccc gcgtctgcca gatagtcggt
      121 gaccacctta gagccggggc cgaggcttgt cttgacccag ggctgtgatt tcaggccgcg
      181 ttccaccgcc ttcttcgcga gcaggccggc ggcgatcatc accgagggat tggaggtgtt
      241 ggtgcagctg gtgatcgagg cgatcacgac cgcgccatgc cccagcgtgt gttccttgcc
      301 atccagagtg caagttgcct gggcgttgat atcactggcg ctgcccttgg ccgcctcaag
      361 gagagccggg agggcggcgg cgaactgccg ctttgcctcg cgcaggggca cgcggtcctg
      421 cggccggcgc gggcccgcga tgctggcttc cacgctgccc aaatcgagcg agagcgtatc
      481 ggagtaggcg gcgacgggcg tatccgcgtc gtggaagagg ccctgctcct tcatgtaggc
      541 ttcgacaagg cgcacctggt gctcgggccg ggcgctcatc ttcaggaagc ggatcgtctc
      601 ggcatctacg gggaagatac cgcaggtggc gccatactcc ggggccatgt tggcaagcac
      661 ggcgcggtcg gccagcgaaa gcgaggccag gccttcgccg tagaactcga cgaacttgcc
      721 caccactcct ttggcgcgca gcatctgcgt gacggtgagg accaggtcgg tcgctgtggc
      781 tccctcgggg agcgcgccct tcagctcgaa gccgaccacc tgggggatga gcatcgaaat
      841 cggctggccc agcatggcgg cctccgcctc gatgccgccg acgccccaac caaggacgcc
      901 caggccgttg atcatcgtgg tgtgcgaatc ggtgccgacg agcgtatcgg ggtaggcggt
      961 gacaatcccg cccttctcgg tggcaaagac gacgcgcgcg agatactcca ggttcacctg
     1021 gtggacgatc ccggtgtccg gaggcacgac ccggaagtta tcgaacgcct tctgccccca
     1081 gcggaggaac tcatagcgct cgcgattgcg ctcgaactcg tgcctggcgt tgatgcggaa
     1141 ggcgtcgggc gtgccgaagg catccacctg aaccgagtgg tcgatcacca gctcgacggg
     1201 ctgcaacgga ttgatgcggc gcgggtcgcc cccgagggcg gccatggcgt cgcgcatcgc
     1261 cgcgaggtcc accacggcgg gcacgccggt gaagtcttgg aggagcacgc gactgggcgt
     1321 gaaggcgatc tcacggctcg gctcggccgc gggatcccag cgcgccagcg cctcgatctc
     1381 ctgcgcggtg acgctgacgc cgtcctcggt gcgcaggagg ttctccagca ggatcttgag
     1441 cgagtaggga aggcgcgcga gcgagaagcc ggcgcgctcc agggcatcca ggcggtagat
     1501 ctggtattcg cggccatcca cgcgcaggga cgaccggctg ccgaaggaat cgggcatctg
     1561 cggatactcc tctttctgcc aggcaatatg gtgttgagct ggccatggaa tgccgcgagg
     1621 gttgcggccc aacaggcctt ggccagctcg atcagggaat agccccattt aaacaaccca
     1681 cccgcttcga gtgggttggg gaaacccggg cacgggccgc aggagcccgg gccacagggc
     1741 cccgatactc tatcttaccg gaaaacggtc gcttcgtcca gatccgcgag gagagccaac
     1801 caggccgcag atggagcatc ggcttgaggc gttggggccg caccggccaa agccggcgcc
     1861 tggaaagcgt tggccggtgg caaacacgag cacgacgtgc ccgccagccc ggccaacgct
     1921 ggcacggaca cgccggaggc ggaccctgtt ccccagaagg atacactctc tcggtgaaaa
     1981 gccctctcgc cgcggggatg cggggcgttc agcgctgctt ggctgccgcc gcggcctttt
     2041 ccgcctctgc ctccttgtac atcgtctcga actcgctggg gttctgcgat ttgtcgaggg
     2101 ccgcctgata cgacaccacc ttgttgcgta ccagatagac caggtactgg tcgaggctga
     2161 tcatcccctg cttcaggccg gtctgcacca tcgagccgat ctggtgcgtc tttccctcac
     2221 ggatcaggtt ggagatggcc ggagtcgctt tcatcgtctc ataggcagcg acgcggccgg
     2281 agccatcggc acgcttcacc agcgtctgcg agatgatgcc gaccaggttg acggagatct
     2341 gcatgcggat ctgcgcctgc tggtgagggg gaaagacatc gatcaggcgg tccacggttt
     2401 gaacggcgtc cgtcgtgtgc agagttgcca ggaccaggtg gccggtctcc gcggcggtga
     2461 cagcgaggga gatcgtctcc aggtcacgca tctcaccgac caggatgaca tccgggtctt
     2521 gtcggagtac ctcgcggagg gcgttggcga aagagtgggt gtcgcggccc agctcgcgct
     2581 ggttgatcag ggctttgatc ggcgtgtgca cgaactccac cgggtcctcg atagtgagga
     2641 tatgcacggg gcggttctgg ttgatgtggt ggaccatcgc ggccagcgtg gtcgtcttac
     2701 ccgagccggt ggggccggtg acgaggacga ggccgcgggg cctcatgcag aactcgaggc
     2761 agacgggcgg gagccccagc tccttggcgg tcttgatcgt gaacgggata acgcgagcga
     2821 ccatggtcac ggttccgcgc tgaaacatca gattgacgcg gaagcgcgcg agcccaggga
     2881 tctcgtgggc gaagtcaagc tcatactcgc gcaagaactg ctctcgctgc tggtccgtga
     2941 ggaggctgaa gacaagctcg cgcaccaact cgtcatgcat cacgggcgtg ccggtgacat
     3001 cggtcatatc gccgaagacg cgggcgacgg gccggcgccc tgacttcagg atcaggtcgg
     3061 atccctgcct gtcgacgaca aacttcatat aggattcgat ctcggctctt cggtcggtca
     3121 tgcccattcc tccgggtgac gacgtggctg ctcggcgaag atccggcgcg gcggtgccgc
     3181 cccgcgtgtc tggggggcga gaggtggccg cgcccggtag agcacaccct tacttccgct
     3241 gctgccccgc ttgcccgctg gcgggcgcct gggaggtcat ttgcgtgggc aacggcaggc
     3301 ccctctgcga gaacgccttc tcgacgcgca tctcgaactc cttggggttc ccgcacttca
     3361 acagcgcttc ctcatacgtc accttgccgc tgacgagcag ctggacgaga tgcccgtcga
     3421 gcgcctgcat gcccagctcg cctccctgct ggatctccgt gggcagctgg tgggtctttc
     3481 cttcgcgaat gagcgaacgg atggcaggcg tggcgatcat gatctcgaac gccgcgatgc
     3541 ggctctggcg gtccgcggtc ggcaacagcg tctggcagat gatcgccagg atggtgacag
     3601 aaagctgcat gcggatctgc gcctgctgat cgggcgggaa gacgtcgatg gcgcggtcca
     3661 ccgtctgcgc tgcgtccgtg gtatgcaggg tgccgaagac gaggtggcct gtttccgccg
     3721 cggtgatggc caggtggatc gtctccaggt cgcgcaactc gccgacgagg atcacgtccg
     3781 gtgcctggcg gaggacgtgc ttcagggcat tctggaagct atgggtatct tcgtgcactt
     3841 cgcgctgctg gacgatgctc ttgatatcct catgcacgaa ctcaatcggg tcctcgatgg
     3901 tgatgatgtg cttggcggtc gtctcgttga tgtagcggat catcgccgcc aaagtggtcg
     3961 tcttgcccga gccggtgggg ccggtgacga ggacgagacc gcgaggccgc agcacaatat
     4021 ccttgatcac tggcgggagg ccgagctgct ccagcgtctt gatctcatag gggatgcagc
     4081 gcatgacgcc gccgacgtac ccctgctggc gcaacgcatt cacgcggaag cgagcgacgt
     4141 ctttcaccga gtaggccatg tccagctcat ttgtctcgag gaacttccgc ttgcgctcct
     4201 cattcatgag gctgaacatc aactcttccg cgtcttccgg tgtgagcggc gggtaattga
     4261 ggggcgtgag gtcgccgcgg atacgaacga gcgggggcag gcccactcga atatgcaggt
     4321 ccgacccgcc gtgctctacg agggctcgaa gcagatcatc catcgtatag ttgagagcca
     4381 tgtccgtctc ctggtgcggg cacggcgccc tcctccggat acctcgccgg cgggcgccag
     4441 tctctatgcc acaggattct ccagactctt ctcattctcc tgttaccagc gcgaacaacg
     4501 gtgtgaggtg ttcccttggg ggcgcaggat gtcttgaggg aggggcgctt atttacggaa
     4561 gcgaagggcc gcggaggcga ccggcgctgc tccacggccc tcaccacgcg cttctatcgg
     4621 cccggggagg gcggtcaacc gcgctggctg gcgcgggccg attccagggc ctgacgcatc
     4681 atctggcgta gctccgtctg gttgccggcg gcgaagagcg cctcatccgc ggagatcagc
     4741 cccgaagtgt agtagcgcag gaggcactgg ttcatcgtct gcattcccca gtgctctccc
     4801 tccgcgatca cggggtaggt ggcgttgaaa cggccttcct cgatcagctt cgccaccgtg
     4861 ggagtgacga ccatcacctc gaccgcggcc attcggccgg agccatccat gcgtgggacg
     4921 agcttctgcg agatcacgcc tcggagcgag ttcgagaggc gctgcgagat catcggcttc
     4981 tcgtgaggcg ggaacatgtt cacgatccgg tcgagcgtct cgggggcgct ggccgtgtgc
     5041 agcgtcgaga agacgaggtg gcccgtctcc gccgcctgga gcgccacggt gaacgtctcc
     5101 aggtcgcgca tctcgcccac catgatgacg tcggggctct ggcgcatcat atacttgagc
     5161 gcgtcctgga agttctccgt gtccactccc acctcgcgct ggctcacgat gcctacctta
     5221 tcgggatgca caaactcgat gggatcctct acggtgataa tgtgcacctt cttcgtgctg
     5281 ttgatcaagt cgaccatcga ggccagggtg ctggac
//
LOCUS       DAOSCV010000194         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9785_length_5309_cov_3.908641, whole genome shotgun sequence.
ACCESSION   DAOSCV010000194 DAOSCV010000000
VERSION     DAOSCV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9785_length_5309_cov_3.908641"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLY56_06160"
     CDS             <1..1017
                     /locus_tag="PLY56_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21098.1"
                     /translation="RCWIRYREEWMYERALAIADFVVESHRPNGRIPEAMDPSSGQWT
                     RLGDGTHYEGVGLCIDALLRLYQLRRDAEHKDDIRLKRAATRFLELALEQRRRRIDRE
                     RRRGKRRHDQETSFPYSGSDITESTNVNMLIILERMRQLTNDRRIDHVREDVEKWVLH
                     NVINDMSWRGGDPIHRGLEARSLLRFLEYAVLRCEATQGKRYLEMAEILASYFMLTLV
                     PKDLEWCAGRTKGLQIANAEWRALTTFYADHVTPVPALHRLGKLCNDRFYAQLADYLV
                     HVAMVAQCDEKGVPGYGGWMPAVNSPDGNAFPLNGLPYDGELCVTSIVPAVLETYLYL
                     TEKK"
     gene            1075..1530
                     /locus_tag="PLY56_06165"
     CDS             1075..1530
                     /locus_tag="PLY56_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21099.1"
                     /translation="MREILGPEEALRWRREALVRTLAVSASLRPARFVGAARGRFGEL
                     LEERSGTRVVRVVPAVVEHYRKHAGQFDLRRAEALLPTVLADPLKVVQGKKATTLVAV
                     GEYDAEHYLVVPVKSLPGENWLETLYIDRKQKFEDRKWVVGGMIFKRRD"
     gene            complement(2019..3386)
                     /locus_tag="PLY56_06170"
     CDS             complement(2019..3386)
                     /locus_tag="PLY56_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21100.1"
                     /translation="MARKNATGLGFWTTFFRMLVGVVLGAGIVTGIVWLIPRYYTSSA
                     VLLFPGAKGSGSAEASSAPSGGSATTDQPAVPLMQGVLSVPRPGTSPSSAALILTSRK
                     VTVDLINRFNLTKEWNRSLERSIQRFHERFVCQEGKAGDLRVSFTDRDPHRARAVVEA
                     AIETLERSVEDLSLDPAARNVDFLRDNVQDAEESCARIQREIVKLQRGLGGSPPDTQV
                     QALSQILNDIQRDLTNAQVEANVAQSSMQAATETAKKMIRAAQDPSGAEGTLIRSLYQ
                     DMVQKEADLALLREKFTDRRAEVVQARQALNIARKSLEKEIARQLESVEKGSSPLVRD
                     AVVQVFTAQARVDGLRQCATEIRERLEDLPAAQVRYAQLQADLRDERSRLSLLRSEYL
                     RARLIVESRGPQFVMLDPPILPKRPNGWELYYWSIAGAIIGGLSVLIVAVTRWLRQSL
                     QDMGM"
     gene            complement(3542..>5309)
                     /locus_tag="PLY56_06175"
     CDS             complement(3542..>5309)
                     /locus_tag="PLY56_06175"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ21101.1"
                     /translation="KASAYARILRFRRFEGGRRVERTLDAQALLADPNHADNMLLRDG
                     DILTLENVTRQVRERVAIRGNVSFPGDYSLQRTPTAKALLLEARPRIGTYWERADLTR
                     CLPDGTPVIIPLPLKDLLDGKAEDVPLLDLDEIVVYEADEKKIVPLATVEGAVKHPAT
                     YRVGDGMRVSDLLFAAGGMLQDASKQVAHLYRRIGPNEFQIVRLAPDAALKGNPAENP
                     VMQDGDRLVIYRQQDVEFRFDRVTAVGELQKPGEYRSYEGMTLYDLLLLAGGPTPEAA
                     GIVEVATPVLDEDSNKRTQIETYALADVMAGKHEPVPIKAGMMVSLPRRDDKLTQPCR
                     VELRGRFRRPGTYALLYEGEDLESLLKRAGGFADDADPFGMSLTRSRDKMLSLATSEQ
                     IRTVMETMDQLLPTRKGAGDGTSVEVLETQSGSGVPLLGGANRTEKVLLVSPRQLTGM
                     PNNTRISFHLEDRESYISRLGKVRLCDGDIIEVPRRSDVVQVLGAVQSPGPVFHSAGR
                     SAREYINLAGGSAPDADLKRSVVIKVTGGVRRLADSKTVDPGDVIVVASKHQIIQPPV
                     QRKLQDTILDLLGVALVIRSLQ"
BASE COUNT          965 a         1732 c         1662 g          950 t
ORIGIN      
        1 cggtgctgga tccgctaccg cgaggagtgg atgtatgagc gggcgctggc gatcgccgac
       61 ttcgtcgtgg agtctcaccg gccgaatggg cgcatcccgg aggcgatgga cccgagcagc
      121 ggtcaatgga cccgcctggg ggacggcacg cactatgagg gcgtagggct ttgcatcgac
      181 gcgctcctcc gcctctacca gctccgccgc gatgccgagc acaaggatga tatccggctc
      241 aagcgcgccg ccacccgctt cctcgaactc gcgctcgaac agcgccgccg ccgcatcgac
      301 cgcgagcgac gccgggggaa gcgccggcat gaccaggaaa ccagtttccc ctactccgga
      361 tccgatatca ccgagtcgac caacgtgaac atgctgatca tcctggagcg gatgcggcag
      421 ctgacgaacg atcggcggat cgatcatgtg cgcgaagacg tagagaagtg ggttctacat
      481 aacgttatca acgacatgtc ctggcggggc ggcgatccca ttcatcgcgg tctggaagcg
      541 cgctcgctac tgcgcttcct cgagtatgcc gttctgcgct gcgaagccac ccaggggaag
      601 cgctacctgg agatggcgga gatcctggca tcctatttca tgctcacgct ggtgcccaaa
      661 gacctggagt ggtgcgccgg acgaacgaag ggtttgcaga tcgccaacgc cgagtggcgc
      721 gcgctcacga cattctacgc cgatcacgtg acccccgtgc cggcgctcca tcgcctcggc
      781 aagctgtgca acgaccgctt ctatgcccaa ctggcggatt acctggtgca cgtggcgatg
      841 gttgcccaat gcgatgagaa aggcgtgcct ggatacgggg gatggatgcc ggcggtgaac
      901 tcccctgatg gcaacgcatt cccgctgaac gggctcccct acgatggcga gctctgcgtg
      961 acatccatcg tgcccgcggt gctggagacc tacctctatc tgaccgagaa gaagtagccg
     1021 ccagcccaca ttcgctacag cgaggaatgg ggcacgacgc gctatgccag gagcttgcgc
     1081 gagatcctgg ggccggagga ggcgctgagg tggcggcggg aagcgctggt tcgcacgctg
     1141 gccgtatcgg cgtcgctacg cccggcgcgt ttcgtcggcg ctgcccgcgg gcggtttggc
     1201 gagttgcttg aggaacggtc tggcacgcga gtggtgcggg tggttcctgc cgttgtggag
     1261 cactatcgga agcacgccgg ccagttcgac ctgaggcgcg cagaggcgct gttgcccacg
     1321 gtgctagcgg atccgctgaa ggtggtgcag gggaagaagg ccacgacact ggtagccgtt
     1381 ggggagtatg acgcggagca ttatctggtg gttccagtga agtcgctgcc cggcgagaac
     1441 tggctggaga cgttgtatat cgaccggaag cagaagttcg aggacaggaa atgggttgtt
     1501 ggcggaatga tcttcaagag aagagactga acgcccggct gggcgggccg ctccagcatc
     1561 aatcccctcg cggggtaatg gtgccctttc cacctcgggc gttcattgac atcattatag
     1621 cagactgagg cgcaactgtc aagttgttca cgagaaatag aagacagggg ccatcttaga
     1681 gggtggcccc tcgtgattct ggggctggtc agaaatggcc ggcccctgct gattccagga
     1741 gcacaacgat ggaagagacc aaggtccagg agaccaacga gagcggccag gagccgcaga
     1801 accagcagca gaccgcattc gtcagaataa tgaaaagaag tctctctgcc aggcgtggca
     1861 cgggttcgtg ggcgctgtcc ccgagaacac tctgcttccg ggtgcttgta gtagcaacgg
     1921 tgtgtcacca gacaagagac ttctcttgaa gtattctgat gaatacttcg ggctagagac
     1981 ctgcagggat cgcgccgggg cgcgatcccc gcttggcgtc acatccccat atcttgcagc
     2041 gactgccgca gccaccgcgt gacggcgaca atcagcacgc tcaggccccc aatgatggct
     2101 ccggcgatac tccagtagta gagctcccag ccattggggc gcttgggcag aatgggggga
     2161 tcgagcatga caaactgcgg gccgcggctc tccacgatca gccgcgcccg gagatactcc
     2221 gagcgcagga ggctgagccg actgcgctcg tcacgcaggt cagcctgcag ctgggcgtag
     2281 cgcacctgtg ccgcaggcag atcctccaga cgctcgcgaa tctccgtcgc gcactgccgt
     2341 aggccatcca cgcgcgcttg cgcggtgaat acctgaacca ccgcgtctct cacgagggga
     2401 gagctgccct tctcgacgct ctcgagctgg cgtgcgatct ccttctccag gctcttgcgc
     2461 gcgatgttga gtgcctgccg cgcctgcacc acttccgccc gccggtcggt gaacttctcg
     2521 cgaaggaggg ccaggtctgc ctccttctgg accatatcct gatagagcga ccggatgagc
     2581 gtgccttctg ccccggaggg atcctgagcc gcgcggatca tcttcttggc ggtctcggtt
     2641 gcggcctgca tgctggactg ggccacgttc gcctccacct gagcgttcgt caggtcacgc
     2701 tggatgtcgt tcaggatctg gctcagtgcc tggacctgag tgtcgggcgg ggagccaccc
     2761 agcccacgct gaagcttgac aatctcgcgc tggatccgcg cgcaactctc ctcggcatct
     2821 tgaacgttgt cgcgaaggaa atcgacattg cgcgccgccg gatcgaggct caggtcctcc
     2881 accgaacgct ccagagtctc aatcgccgct tccaccaccg cccgcgcccg atgaggatcg
     2941 cggtcggtga aactcacccg caggtcaccc gccttcccct cctggcagac gaaccgctca
     3001 tgaaaccgct ggatggagcg ctcgagcgag cggttccact ccttggtgag gttgaagcgg
     3061 ttgatcaggt ccaccgtcac tttgcggctg gtcaggatca gcgccgccga cgaggggctc
     3121 gttcccggtc ggggcaccga gagcacccct tgcatcaggg gaacggcagg ttggtccgtg
     3181 gtcgcactcc ccccggaagg tgcgctcgat gcctctgcac tgccgcttcc cttggcgccc
     3241 gggaagagca acaccgcaga agaagtatag tagcgcggga tcagccaaac gatcccggtg
     3301 acgatccctg ctcccagaac tactcccacg agcatccgga agaaggtcgt ccagaacccg
     3361 aggccagttg cgttcttacg tgccatgtat gattgccttc gctgattgca gccttaccag
     3421 accggttgcc tccggcaccc cacgccatgg aaaggcatcc aggcgcgcag tagccggcga
     3481 ccacccgcgc ctgtgcgcgg tcgccctccg tgtccgtcgg tttccacctg tggctgcatc
     3541 cttactgcaa gctgcggatc accaacgcaa cgcccagcag gtcgaggatg gtatcttgca
     3601 gcttgcgctg caccggcggc tggatgatct ggtgcttgga ggccaccacg atcacgtcgc
     3661 cgggatcgac cgtcttgcta tccgcgagcc ggcgcacccc accggtgacc ttgatgacca
     3721 cgctccgctt cagatcggca tccggtgcgc ttcccccggc caggttgatg tactcccgcg
     3781 cgctccgccc agcgctgtgg aacaccgggc ccggcgactg caccgcgccc agcacctgaa
     3841 ccacgtcgct acgccgcggc acctcgatga tgtccccatc gcagagccgc accttgccca
     3901 gccggctgat atacgattcc cggtcctcca gatggaagct gatccgggtg ttgttcggca
     3961 ttccggtgag ctgccttggc gagacgagca gcaccttctc cgtccggttc gcgcccccca
     4021 agagtggcac accggaaccg gattgcgtct ccagcacctc gacgcttgtt ccatcccccg
     4081 cgcccttgcg cgtcggcagg agctggtcca tcgtctccat cacggtgcgg atctgctcgg
     4141 aggtcgccag cgagagcatc ttgtctctgg agcgcgtcag cgacatgccg aagggatccg
     4201 cgtcatcggc gaagccaccg gcgcgcttca gcagcgactc caggtcctcg ccctcataca
     4261 agagcgcata ggtgcccggg cggcgaaaac gcccacggag ttccaccctg cacggttggg
     4321 tcagcttatc atcgcggcgc ggcagcgaga ccatcatccc cgccttgatg ggaaccggtt
     4381 cgtgctttcc agccatcaca tcggccaggg cgtaggtctc gatttgggtg cgcttgttcg
     4441 aatcctcatc cagaacaggc gtcgccactt ccacgatacc ggcggcctcc ggcgtcggtc
     4501 ccccggcgag cagaagaagg tcgtagagcg tcatcccctc atacgagcga tactcgcccg
     4561 gcttctgcaa ctcgccgacg gcagtcactc tatcgaagcg gaactcgacg tcctgctggc
     4621 ggtagatcac cagccggtcg ccgtcctgca ttaccgggtt ctccgccggg tttcccttca
     4681 gcgcggcatc cggcgcaagc cggacgatct ggaactcgtt cggtccgatg cgccggtaca
     4741 ggtgcgccac ctgcttcgaa gcgtcctgca acatgccgcc cgccgcgaag agcaggtccg
     4801 aaacccgcat cccatcgccc acccgatacg tcgcagggtg cttcaccgca ccctcgacgg
     4861 tagccagagg aacgatcttc ttctcatccg cctcgtagac cacgatctca tccaggtcga
     4921 gaagcggaac atcctcggcc tttccatcca ggagatcctt cagaggcagc gggatgatga
     4981 ccggcgtgcc atccgggagg cagcgggtca gatcggcgcg ttcccagtag gtgccaatcc
     5041 gcggccgcgc ctccagaaga agtgccttcg cggtgggcgt gcgctgcaac gagtagtcgc
     5101 ccgggaaaga aacgtttccg cggatggcaa cacgctcgcg cacctgacgg gtgacgtttt
     5161 ccaacgtgag gatatcgcca tcgcgcagga gcatgttatc ggcgtggttt gggtccgcga
     5221 gcaacgcctg cgcgtcgagg gtgcgctcca cgcgacgccc gccctcaaac cgccggaagc
     5281 gaagaatgcg cgcgtacgca gacgccttc
//
LOCUS       DAOSCV010000195         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9831_length_5291_cov_3.672460, whole genome shotgun sequence.
ACCESSION   DAOSCV010000195 DAOSCV010000000
VERSION     DAOSCV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9831_length_5291_cov_3.672460"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..439)
                     /locus_tag="PLY56_06180"
     CDS             complement(152..439)
                     /locus_tag="PLY56_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21102.1"
                     /translation="MKLLTLVYDADLEDQITSVFQRSMAVTRYTKIEGVVGARMDALA
                     ESDYATDRRNNMVLVVADEETMELIVRELRALRDQVGHGMRGVVTRAEALI"
     gene            complement(635..2404)
                     /locus_tag="PLY56_06185"
     CDS             complement(635..2404)
                     /locus_tag="PLY56_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOJ21103.1"
                     /translation="MPDIELLETINVRGAHGQIYKIRVRSQSPAGQRLAAMKVPTTPD
                     RGFVREMEIAELLRHYQHPYIIRFIATGIPTDESFILMDLHEEGDLEDLIRMHGPLPI
                     REALDLLIQVAEAIHFMHGLGIYHRDIKAENIVISQTGAPKVMDFGTARVYRRRGGIL
                     LPGPEALASERQVDRRRDARMLGEVLYHALSGTKPYEARLFALAQRRRLAPAASTRQD
                     AHRHPALYATVDAVLRRALDPGYTQVYEGVRYFLSDLRRLRQECEREEQPLPRTTPLH
                     ERLLPTGPGFLERARQEAWAATEREPDNAAHWIHLGNAHLVALQARAARRAYAQALQR
                     DPGNVIARLNQAYAEALLGHWESAMHLAISGAAAAPSELGAVARARHVREARHLVQDA
                     AELYAILDDRLPFNRLMRGYAYDAQGAGQSASQEYRKALELDPAYSPAAEALAFLAIR
                     QGDPDAAMAHALAAVRIGQERAEAWLALGRAHLARGEPHDAAVALTTAVGLHPNHPYC
                     RRALAEAWTALGEAERAEDEMRHVVRIDPCWCEARLAWGDALRTLGRHADGEAAYRTA
                     VSSNPECIDADGRPGLSFALDTL"
     gene            complement(2415..3203)
                     /locus_tag="PLY56_06190"
     CDS             complement(2415..3203)
                     /locus_tag="PLY56_06190"
                     /inference="COORDINATES: protein motif:HMM:NF027034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOJ21104.1"
                     /translation="MTDNETMERLTWLRICGPEGAHTRQNNLESIGRMKSGDPYVTLG
                     IAAVEEAVRNGERWNTTALLDAIASVTGCSKDPRHTVGQGYISPRATLRGLRECARKV
                     RETALRGGSIFFGTGHAGCLVACYQVIADAARELGAEIVHAADGLEVQSWEFVDWVGD
                     VCVVSNGSGLLHTHTQAAAEEVISDWGKVDLAVTDHGFLGPIVNRGIPTVAFFDTNDP
                     APALAYRLGLDITLVPLNDNRPNAILREAGLVVAEEIRNQVLVA"
     gene            complement(3345..4190)
                     /locus_tag="PLY56_06195"
     CDS             complement(3345..4190)
                     /locus_tag="PLY56_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOJ21105.1"
                     /translation="MNVALNHAGQEPAERDQRHPVAVEILAVGNELLDGITQDTNTHW
                     LCRQITALGAAVRRAVVVRDDTQAIAEEVRGARDRGTALLLVMGGLGPTADDLTLQGV
                     AMAAGQPLEEHPDARAMVAARYEALYRDGAVATAEVTPDRLKMAILPRGAEPVHNPVG
                     AAPGVLLHLGETTVVCLPGVPRELKAIFEGPLRPTLLSILGAGVAREREVLVASNDES
                     ALAPLIREVVALFPDLYIKSHARDFESGHGIRVTLSGRGTDPVRLDQRLDEAVRALRD
                     RLPTS"
     gene            4246..>5291
                     /locus_tag="PLY56_06200"
     CDS             4246..>5291
                     /locus_tag="PLY56_06200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ21106.1"
                     /translation="MAENKRPKTLRLVDRVLARLSRVTSSGNFIPEIDGLRFFAIAAV
                     VLFHIAEHVRFHGPVHYADAPRSNIYRLCSAGFVGVELFFAISGFILFLPFAAHFLRG
                     GKQPRLRQYYFRRVTRIEPPYLIHLGIAFVIALVINDRDPLQLAPHLMASMFYLHNLI
                     YGMSSTLNMVAWSLEIEVQFYLLAPLLAGLYRIRHSLQRRLAWLALTMSAMAWRPCLS
                     SPLHCSLLAYLPYFLVGFLLADLFVTTWNRPPTATWKQDLMATGAWLLLIPATELDAW
                     RQWILPPLILIAYVGALRGRYWRQIVSNCWLVVTGGMCYTIYLYHVFIIGLVGRVASG
                     VMVTQLYWANFLLFL"
BASE COUNT          837 a         1765 c         1739 g          950 t
ORIGIN      
        1 gggtttccgg aggcgagatc tccggctggc cggcgtcatc cggctcgaac gcgaggaggg
       61 cgcgcgcgcc gatggagagc gtgagggcta cggcaacgct cgcaaccggc atcaccccgc
      121 gacgcgggca ttctctcccg ttgcggttca ttcagatgag tgcttccgcg cgggtgacaa
      181 caccgcgcat tccgtggccc acctgatcgc gcagggcgcg caactcgcgg acgatgagct
      241 ccatcgtctc ttcatcggcg actaccagaa ccatgttgtt cctccgatcg gtggcgtagt
      301 cgctctcggc cagggcatcc atgcgcgcgc cgaccacacc ctcgatcttg gtgtagcgtg
      361 tgaccgccat actgcgctgg aagaccgagg tgatctgatc ctccagatcc gcatcgtaca
      421 cgagcgtaag caacttcatc cgcgcttatc cttcccgggc caccggggcc aatgcaatcg
      481 tttgtttatc agtctccccc tgattctacc agaggtcggc acgggggtca agcggcagct
      541 ggatgttcag gaagtgggga gaagggcgtg gccggcggcc ccgcggcttt gtcactggca
      601 ttccactcct caggcgggat ttgacatggc ccggctagag ggtatcgagc gcgaaggaga
      661 gcccgggacg gccgtcggca tcgatgcatt ccgggttgct ggaaaccgcc gttcggtagg
      721 cggcctcgcc gtcggcgtgc cggccgaggg tgcgcagggc atccccccag gccaggcggg
      781 cctcgcacca gcagggatcg atccgcacca cgtggcgcat ctcatcttcg gcgcgttccg
      841 cctcgcctag ggcggtccag gcctctgcga gcgcccgccg gcagtagggg tgattcggat
      901 gcaggccgac ggcggtcgtg agcgccactg cggcatcatg gggttcgccg cgggcgaggt
      961 gcgcgcgacc cagggcaagc cacgcctccg cgcgttcctg accaatgcga accgcggcga
     1021 gggcgtgcgc catggcggca tcggggtctc cctgtcggat cgccagaaag gccagcgcct
     1081 cggccgccgg agagtaggcg ggatcgagct ccagtgcctt gcgatactcc tgagaggcac
     1141 tttggccagc gccttgggca tcgtaggcat acccccgcat cagtcggttg aagggcaggc
     1201 gatcatccag gatggcgtac agctcggcgg cgtcttgcac gaggtgacgt gcctcgcgca
     1261 cgtgccgggc gcgcgcaacg gcaccgagct cactgggtgc ggcggcagcg ccactgatcg
     1321 ccaagtgcat cgcgctctcc cagtgaccaa ggagcgcctc cgcatacgcc tgattgagcc
     1381 gggcgatcac gttgcccgga tcccgctgga gcgcctgcgc ataggcgcgg cgcgctgccc
     1441 gagcctggag agccaccagg tgcgcgtttc ccagatggat ccagtgagcc gcgttgtccg
     1501 gctcgcgctc ggtcgccgcc cacgcttcct ggcgggcgcg ctccaggaac cctgggccgg
     1561 tcggcagcag gcgttcatgg agcggcgtgg tacgcggcag cggctgctcc tcccgctcgc
     1621 actcctgtcg caggcggcgc agatcggata gaaagtatcg gacgccttcg tagacctggg
     1681 tatagcccgg gtcaagcgcg cgccggagga cggcatccac ggtggcatac agcgcgggat
     1741 ggcggtgagc atcctggcgc gtgctggccg cgggcgccag tcgccggcgc tgcgccaggg
     1801 cgaagagcct cgcctcgtac ggcttcgttc cggagagggc atgatacagc acctcgccca
     1861 gcatgcgcgc atcccgccgc cggtcgacct gccgctcgct ggcgagtgcc tccggccccg
     1921 gcaggagaat gccaccccgc cttcggtaga cgcgcgcggt gccgaaatcc atcaccttgg
     1981 gcgcgcccgt ctgcgagatg acgatattct ccgccttgat atcgcggtgg tagattccga
     2041 gcccgtgcat gaagtgaatt gcctcggcga cttgaatgag gagatcgagc gcctcgcgta
     2101 tggggagggg gccatgcatc cggatcagat cctctagatc gccctcctcg tggagatcca
     2161 tcaggatgaa cgactcgtcg gtggggatgc cggtggcgat gaaccggatg atgtagggat
     2221 gctggtagtg gcgcagcagc tcggcgatct ccatctcgcg cacgaaaccg cggtcaggcg
     2281 tggttggcac cttcatcgcc gccaggcgct ggccggccgg cgattgcgag cgcacccgga
     2341 tcttgtagat ctgtccgtgc gcgccgcgaa cgttgatcgt ctccaggagc tcgatgtcag
     2401 gcaccctctg ctcctcacgc gacgagaacc tggttgcgga tctcctcggc aaccacgagc
     2461 ccggcctccc gcaggatcgc gttgggccgg ttgtcgttta gtggcaccag ggtgatatcg
     2521 agacccaggc ggtaagcgag ggcgggcgcc ggatcgttcg tgtcgaaaaa cgccaccgtc
     2581 gggatgcccc ggttgacgat cgggccgaga aagccgtggt cggtgaccgc caggtccacc
     2641 ttcccccaat cgctgatcac ctcttcagcg gcggcctgcg tgtgcgtgtg caacaggccg
     2701 ctgccgttgc tcaccacgca cacatctccc acccagtcca cgaactccca tgactggact
     2761 tccaggccat cggcggcatg cacgatctcc gcgcccagct cgcgcgcggc atcggcgatt
     2821 acctggtagc acgccaccag acacccggca tgccccgttc cgaagaagat cgatcccccg
     2881 cgcaacgctg tctcgcgcac ctttctggcg cactcacgca gcccgcgaag tgtggcgcgg
     2941 gggctgatgt agccctgacc gacggtgtgc cttggatcct tggagcagcc ggtgacgctg
     3001 gcgatggcgt ccagcagcgc cgtggtgttc cagcgctcgc cattgcgcac cgcctcctcg
     3061 accgccgcga tgcccagggt gacgtaggga tcgcccgact tcatcctgcc gatcgattcg
     3121 aggttgttct ggcgcgtgtg agcgccctct ggcccgcaga tgcgcagcca ggtgagccgc
     3181 tccatcgtct cgttatctgt caccgctgcc gcccctcctc atcaacgcga agtgagaccg
     3241 ccgcagccgc gctgccctgc cgtagcaaac agtgatttga tgcggctttt ccccggtgcg
     3301 ggaagcgcga aacgcggggc gcgagcggcc tccgggaagg gacgtcagga agtgggcaga
     3361 cggtctcgca gagcgcgcac cgcctcgtcg agacgctggt cgaggcggac gggatcggtg
     3421 ccgcgccccg agagcgtgac gcggatcccg tgtccagact cgaagtcgcg cgcgtgcgac
     3481 ttgatgtaaa gatcggggaa gagcgcgacc acctcgcgga tgaggggtgc cagggccgac
     3541 tcatcattgc ttgcgacgag cacctctcgc tcgcgggcga cacctgcgcc aaggatcgag
     3601 aggagcgtcg ggcgcaacgg accttcgaaa atcgccttca actccctcgg cacgcccgga
     3661 aggcagacga ccgtggtctc ccccaggtgc aggagcaccc caggcgccgc gccgaccggg
     3721 ttgtggaccg gctccgcgcc tcgcgggagg atcgccatct tcaaacggtc gggggtgacc
     3781 tccgccgtgg cgacggcgcc gtcgcggtaa agcgcctcgt agcgggcggc taccatggct
     3841 cgggcatcgg ggtgctcctc cagcggctgc ccggcagcca tcgccacgcc ctggagggtg
     3901 agatcgtcgg ctgtgggccc caacccgccc atgaccagga gcagcgcggt gccgcggtct
     3961 cgcgcgccgc gcacctcttc cgcgatcgcc tgggtgtcgt cgcgcacgac gaccgcgcgg
     4021 cgcaccgccg cacccaatgc cgtgatctgg cggcagagcc agtgagtgtt ggtatcttga
     4081 gtgatgccgt cgagaagctc gttccccacg gccaggatct caacggcaac agggtgtctc
     4141 tgatctcttt cggcgggctc ctggcccgca tggtttagtg ccacattcat cggaatctgt
     4201 ctcaaagagg gttaggtgcc acgatacgtg ggtagaccac acggtatggc agagaacaaa
     4261 cgtccgaaga cgctccgctt ggtggaccga gtgctggctc ggctctctcg ggtgacttca
     4321 tccgggaact tcatcccgga gatcgatggc ctgcgcttct ttgcgatcgc cgccgtcgtg
     4381 ctgttccata tcgccgagca tgtgcgattc catggcccgg tacactatgc ggatgcaccg
     4441 cggagcaaca tctaccggct atgcagcgcg ggcttcgtcg gcgtggagct tttcttcgcc
     4501 atcagcgggt tcatcctatt cctgccgttc gctgcgcact tcctgcgagg ggggaagcag
     4561 cccaggctgc gccagtacta cttccgacga gtcacgcgca tcgagccgcc ctacctcatc
     4621 cacctgggta tcgccttcgt gatcgcgttg gtgatcaacg accgtgatcc actgcagctc
     4681 gcgccgcatc tcatggcgag catgttctac ctgcacaacc tgatctatgg gatgagcagc
     4741 acgttgaaca tggtggcgtg gtcgctggag attgaggtcc agttctattt gttggcgccg
     4801 ctactggcag gcctctaccg catccgccac tcgcttcaga ggcgcctggc ctggctggcc
     4861 ctcacaatga gcgccatggc ctggcggccc tgcctctcct cgccactcca ctgctcgctt
     4921 ctggcctacc ttccctactt cttggttggt ttcctgctgg ctgacctctt cgtcaccacc
     4981 tggaaccggc ctccaacggc aacatggaag caggatctga tggcaaccgg ggcctggctg
     5041 ctgctgatac cggccaccga gctggatgcc tggcggcaat ggattctgcc gccgctgatc
     5101 ctgatcgcct acgtgggtgc actccggggc aggtactggc gccagatcgt cagcaactgc
     5161 tggctggtcg ttaccggcgg catgtgctac acgatctacc tgtaccacgt cttcatcatc
     5221 ggcctggtgg ggcgcgtggc aagcggggtg atggtcacgc agctctattg ggcgaacttt
     5281 ctcctcttcc t
//
LOCUS       DAOSCV010000196         5291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9832_length_5291_cov_3.316272, whole genome shotgun sequence.
ACCESSION   DAOSCV010000196 DAOSCV010000000
VERSION     DAOSCV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9832_length_5291_cov_3.316272"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLY56_06205"
     CDS             complement(<1..298)
                     /locus_tag="PLY56_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21107.1"
                     /translation="MERIPVIMIRADLEGLPSFPLPAGYRIRCYRQGEESHWAEVESA
                     AGEFQSSERALARFLEEFGGALAEMEERCFFLETDAGRVIGTATAWYHPNFRGAR"
     gene            complement(346..1038)
                     /locus_tag="PLY56_06210"
     CDS             complement(346..1038)
                     /locus_tag="PLY56_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ21108.1"
                     /translation="MAEQFAVTAGNEEYLEWIYRLSKEKSTVNPLDLARTLKVSPASV
                     TAMLKRLSAASLIEYQPYQGITLTERGREIAMRMVRRHALLERLMTDILGLPWEKADE
                     LACQLEHYVDEEVDERLATLLGNPTTCPHGRQIDLESPDHTCPLTEVSLGEEVEIAYV
                     SDERSDLLGYLATLGLRPGVRVRLTHQAPFNGPLTLRVGEVSHAVGREAAEKVRVHLL
                     PTTADEPASSSE"
     gene            complement(1115..1300)
                     /locus_tag="PLY56_06215"
     CDS             complement(1115..1300)
                     /locus_tag="PLY56_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21109.1"
                     /translation="MEKREEPREREKAWRPEKPVGGGPESPGGDTEPSNWPGETRERG
                     MRPPDPQPILPNEPDER"
     gene            2056..3060
                     /locus_tag="PLY56_06220"
     CDS             2056..3060
                     /locus_tag="PLY56_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21110.1"
                     /translation="MSIEARLSKLQSLVEETFLLWDQVRIGWSWRHYYLNHTLRVRAL
                     ALELGKHEGADPDVVAFAATLHDITKRYDGNVIMGPDGKRLVDKNGYWMTETLQPTRE
                     NRVTRLYDALGLAGQPHHYSGAVLAEHLLTEEGFSPEFAASVAAVIRAHVAPQTASPE
                     EREALYRAPEARVLSDADLIDANLGLVAFYRNIQIHAGRMLQQTGCVDPGAYIEVVER
                     WVNSKDSFLESLLTPSGREVAAQRQARNRQLVEWIAEERALGPVAWEYGLLGVIAFLL
                     EDSYDPCLTRALRLLEELWLPNRQARLSNDGSSPHARALLVRSDQCLNLLHEEVAGRL
                     "
     gene            complement(3158..>5291)
                     /locus_tag="PLY56_06225"
     CDS             complement(3158..>5291)
                     /locus_tag="PLY56_06225"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOJ21111.1"
                     /translation="PAVLRAEYLDEEFCESQTCPYIQVLPEFIDAALQLGKAEPQVLT
                     PRLYFDRGRRHLLRALGGMIRQMQQPSSEYARRDGQVPRERGSERTRPAPPLPPISAG
                     DIRRALDTALDTLSRFRRRVAARGEEILRSLPADATAFVVLGRPYTLHDPAVNMHIGK
                     KIQDLGILAIPQDFLPLPQAKAADAWEGLYSRQIQNRIAAARIIRKDPRLRAVVLTYF
                     GCGPDSFGNQFLRDELNEPCYVMQLDEHTADAGVITRIEAFADTVRAPRTPEPFVPFT
                     TDSIHPNSLEGRTLWIPDACAAARVLAATFRAYGIDARVLPRSPDPALNLARAAIPED
                     VCVPALYTTEDILYRIRQPDFKPEQEAFFQGNAAGPCRYGMYSLLQRRILDKENLPQV
                     DIATVGARYVGGGLSTSSSLVAWDSLVTHDLLEKMLLRTRPYEKNPGEADALFNRLLE
                     ELCAEIKPHRDLVESLTGKLSILSGRHLKRFRDLLRRAQEEFARIPRREEERPLVGVI
                     GEFYVRLHDRSNQDLIRRLERGGVEVWLAPLTEFLGYANRIKHELAGDEYRDTRSGDA
                     FRRWALCGLQDWLAVHDEHLLWEAALPYLEGYDEMTPGTLIDRGEEFIHRSFGGEAIC
                     SMGKAVDFVHRGADGIVSAIPFNCMPGISVQAISRALRRRYGGIPFLTLDYDGFADSG
                     RESRLASFLALVKERRAARNGQKCHSRV"
BASE COUNT          943 a         1709 c         1706 g          933 t
ORIGIN      
        1 agcgcgcgcc gcggaagttg ggatggtacc acgccgtcgc ggtaccgatg acgcgcccgg
       61 cgtccgtctc caggaagaag cagcgctctt ccatctccgc gagagccccg ccgaactcct
      121 ccagaaagcg cgcaagcgcc cgctcggaac tctggaactc gcccgccgcg ctctccactt
      181 cagcccagtg ggactcttcg ccctgccggt agcaccggat ccgatagccg gcgggaagcg
      241 ggaaggaggg caggccctcc aggtccgcgc ggatcatgat gacgggaata cgctccatcg
      301 cctcgctccc tcaccgagaa tcgggagtga cgtaccggcg cgccctcact cggaggagga
      361 tgccggctcg tccgcagtag ttggcaagag gtgaacgcgc accttctcgg cagcctctcg
      421 gccgacggca tgtgaaacct cgccgacgcg caaggtgagc ggcccgttga agggagcctg
      481 gtgcgtgagg cgcacgcgca ccccggggcg cagcccgagc gtggcgagat agcctagaag
      541 atcgctccgc tcgtcgctga cgtaggcgat ctcgacctct tcgcccagcg agacctccgt
      601 gagggggcag gtgtgatccg ggctttccag gtcgatctgc cggccatgcg ggcaggtggt
      661 gggattgccc aagagcgtgg ccagccgctc gtccacctcc tcgtcaacgt agtgctcgag
      721 ttggcaggcg agctcatccg ccttctccca gggaagacca aggatatcgg tcatcagccg
      781 ctcgaggagg gcatgacgcc ggaccatgcg catcgctatc tcacggccgc gctcggtgag
      841 ggtaatcccc tggtaaggct gatattcgat caggcttgct gccgacaggc gcttgagcat
      901 cgccgtcacc gaggccgggg agaccttcag ggtccgtgca aggtccagcg gattgaccgt
      961 gctcttctcc ttcgaaagcc ggtagatcca ctccagatac tcctcattcc ccgccgtcac
     1021 ggcgaactgt tcagccacag cagctccctt cctcaaaggc gattatacgg cgggctgcgt
     1081 ggtttgtcaa atggaatgag gcaggaagcc actttcagcg ttcatccggc tcgttgggaa
     1141 gaatcggttg cggatcgggg ggccgcatcc cgcgctcgcg cgtctctccc ggccagttcg
     1201 acggttcggt atctcccccg gggctctccg gccctccgcc taccggcttc tccggtcgcc
     1261 acgccttctc tcgctctcgc ggctcttcgc gcttctccat cggtcaacct ccaccaggag
     1321 gcgcgccccg gggccttctg gatacacgcc aagaaccatc caggcccggc gcgtcctttc
     1381 cacgcatccc gaggctgttg cccctgatgg agacctattg ccagacacgc gggaatccaa
     1441 acgatgcccg cgcctactcg catttctgga tcagtggcga ttccaaggat tggtcccccg
     1501 ggcccccgcc tcacaaggct ctcatgcagg cgacgcgccc ggtgccctgc gtgttgaaat
     1561 ggttacgcac gcagctgcag cacgtgccct tattgccgca cggttcgtag gtgcagatac
     1621 agaagtcgag gttgcgcgaa aggttggtgg tgcatttcac tgcctggagg gagagcgcca
     1681 tcgtctctgg agcgcgcttt gccccccgca tgcaggagac cagactcttg ttgccggcgt
     1741 gcctttgcaa gcattcgcag cagatgccat ggtttgcgca tgtcgtattc gtgcaggggc
     1801 acatctgcgt gttgatctcg tagtttggac agttttccct cgcagtaggc atggcactta
     1861 tctcctcgtg gaaggctggg tgacaacgct cgcaccggca agcgccggtg tccactacgc
     1921 ggttcggtag cagccgcccg aactcctacc agcccccgac accgtctgcc tgcggagcga
     1981 gaaggcccga acgcccctcg cggcgaatcc ttttccggcg cgaaatccgc gccagaacgg
     2041 tgaatgggag agaagatgtc aatcgaggcg cggctgtcaa agctccagtc gctggtggaa
     2101 gagacgtttc ttctctggga tcaggtgcgc atcggctggt cctggaggca ctactacctg
     2161 aatcacacgc tgcgcgtgcg tgccctggct ctagagctgg ggaagcacga aggtgccgac
     2221 ccggatgtcg tcgccttcgc tgccacgctc catgatatca ctaagcggta tgacggcaac
     2281 gtcatcatgg gcccggatgg caagcgactg gtcgacaaaa acggctactg gatgacggag
     2341 acgctccagc cgacgcgcga gaaccgcgtg acgcggctct acgacgcgct gggtctcgcc
     2401 ggccagcccc accactattc cggcgccgtg cttgccgagc atctcctgac cgaggagggc
     2461 ttctctccgg agtttgccgc ttccgtcgcc gccgtcatcc gggcgcatgt ggccccgcag
     2521 actgcctctc cggaggagcg tgaggccctc taccgggctc ccgaggcacg cgtgctcagc
     2581 gacgccgacc tgatcgatgc caatctcggc ctggtcgcct tctatcgcaa catccagatt
     2641 cacgccggcc ggatgctcca gcagacgggc tgcgtggatc ctggcgctta catcgaggtc
     2701 gtcgagcgct gggtcaatag caaggattcg tttctagaga gcctgctcac tccttcaggg
     2761 cgcgaggtgg cagcgcaacg ccaggcgcgc aaccgccagc tggtggagtg gatcgccgaa
     2821 gaacgcgcgc tggggccagt ggcatgggag tatggtctac tcggagtgat cgcgtttctc
     2881 cttgaggata gctatgatcc ctgtctgacc agagctctcc gacttctaga agagctatgg
     2941 ctccccaatc ggcaggcgcg tctctcgaac gatggctcct ctccacacgc gagggcgctc
     3001 ctggtgcgtt ccgatcagtg cctgaacctg cttcacgagg aggttgcggg gcgcctctaa
     3061 gcaccggcgc ccggatgctc ttaggtgaga gggttaggat aagagccgtg gcggcggggg
     3121 cacctcccgt gggagacgtc cccgccgcgc cgcttttcta gacgcgcgaa tggcacttct
     3181 ggccgttgcg ggccgcccgg cgctctttca ccagggcaag gaagctagcc aggcggcttt
     3241 cgcgcccgct atcggcgaat ccgtcgtagt cgagagtgag gaagggaatg ccgccatagc
     3301 gccggcgcaa agcgcgggag atggcctgaa cgctgatgcc cggcatgcag ttgaacggga
     3361 tcgcgctgac aatgccatca gcgccgcggt ggacgaaatc caccgccttc cccatgctac
     3421 agatcgcctc gccgccaaag gagcggtgga tgaactcctc gccacgatcg atcagtgtgc
     3481 cgggggtcat ctcgtcgtag ccctccagat agggaagcgc cgcttcccag aggaggtgct
     3541 catcatgcac agcgagccaa tcctgaaggc cgcagagcgc ccaacgccgg aacgcatcgc
     3601 cgctgcgggt atcgcggtat tcatcgcccg cgagctcgtg cttgatgcga ttggcatagc
     3661 cgaggaactc ggtgagcggc gccaaccaca cctccacccc tccccgctcc agccgacgga
     3721 tgagatcctg gttggagcgg tcgtgcagac ggacatagaa ctcgccaatg acaccgacca
     3781 agggccgctc ttcctcacgg cgcgggattc gggcaaactc ttcctgcgcg cgccggagga
     3841 gatcccggaa gcgcttcagg tgccgccccg agaggatgga aagcttccct gtgaggctct
     3901 cgaccagatc gcgatgcggc ttgatctcag cgcacagctc ttcgagtagc cggttgaaga
     3961 gggcgtctgc ctcgccaggg ttcttctcgt aggggcgggt gcgcaggagc atcttctcca
     4021 acaagtcatg cgtcaccagg ctatcccacg ccaccagcga ggaggaggtg cttaggccgc
     4081 caccgacata gcgcgccccc acggtcgcga tatccacctg ggggagattc tccttatcga
     4141 ggatacggcg ctggagcaga gagtacatgc cgtagcggca tgggcccgcg gcattgccct
     4201 ggaagaaggc ctcttgctcc ggcttgaaat ccggctggcg aatgcggtaa aggatatcct
     4261 cggtggtgta tagcgccggc acgcacacat cctcggggat cgctgcgcgc gccaggttga
     4321 gggccggatc gggcgagcgc gggagcacgc gtgcatcgat gccataggcg cggaaggtgg
     4381 cagcgagcac tcgcgcggca gcgcaggcgt ccggaatcca gagcgttctc ccctccagcg
     4441 agttcggatg gatcgaatcg gtggtgaaag gaacgaacgg ctcgggagta cgcggcgcac
     4501 gcacggtatc ggcaaaggcc tcgatgcgag tgatcacgcc cgcatccgcc gtgtgctcat
     4561 ccagctgcat gacgtagcac ggctcgttga gctcgtcgcg caggaactgg ttgccgaagg
     4621 aatcggggcc gcagccgaag taggtcagga cgacggcacg cagtcgagga tccttccgga
     4681 tgatgcgcgc ggcagcgatc cggttctgga tctggcgcga gtagaggcct tcccacgcat
     4741 cagccgcctt cgcttgcggc aacggcagga agtcctgggg gatcgccagg atgcccagat
     4801 cctggatctt cttgccgatg tgcatattga cggcgggatc gtggagcgtg tagggccggc
     4861 ccaacacgac aaaggccgtc gcatcagccg ggagtgaccg gaggatctcc tcgccgcgcg
     4921 cggcgacacg ccgccggaag cgagagagcg tatccagagc cgtatcaagg gcgcggcgta
     4981 tatcgcccgc tgagatgggt ggcagtggcg gagccggcct ggtcctctcc gaaccccgct
     5041 cccgtggcac ctggccatcg cgcctggcat actcggacga cggctgctgc atctggcgga
     5101 tcatcccccc aagagcgcgt agcaggtggc gacgaccccg gtcaaagtaa agccgcgggg
     5161 tgagtacctg gggttcggct ttgcccagct gcagggccgc atcgatgaac tccgggagca
     5221 cctggatata ggggcatgtc tgactctcgc agaactcctc atcaaggtac tcggcacgca
     5281 gcaccgccgg c
//
LOCUS       DAOSCV010000197         5288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9841_length_5288_cov_3.234474, whole genome shotgun sequence.
ACCESSION   DAOSCV010000197 DAOSCV010000000
VERSION     DAOSCV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9841_length_5288_cov_3.234474"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLY56_06230"
     CDS             <1..233
                     /locus_tag="PLY56_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ21112.1"
                     /translation="DLDNQAIATHPEVRQLLRAEIDRLSDGLAEFERVKRFAVLDREF
                     SIELGELTPSLKPRRGVILERFAPQVNSLFRS"
     gene            complement(241..1548)
                     /locus_tag="PLY56_06235"
     CDS             complement(241..1548)
                     /locus_tag="PLY56_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ21113.1"
                     /translation="MLPELRTEVPGPRSREMSRELRRWESPNITYVSPDFPIFWESAS
                     DCLVTDVDGNTFLDLSAGFGAAAVGHANPRVVTAIQAQASRLPHGMGDVHPPAVKVEL
                     LRALARVCPGRLEQAILGSSGGESIEAALKTAAVATGRAGVIAFEGAYHGLTYGALAA
                     TGRDDFRRPFREQLGRFVRHLPYPVDATEAARVLEQVAEWLRCPPPDLPPVGAVLVEP
                     IQGRGGIRIPPSGWLSDLLALCHERGALLIADEIFTGFGRTGTWFASEVVPDILCVGK
                     AMTGGFPISACVSTPEIMATWGESQGEALHTSTFLGNPLGCAAALAAIAEIEKRGLVE
                     RCRQLGEWLRPRLEALCERHALVAEARGRGLMWGLELRDASGTPATHAAARVVVEALR
                     RGVILLPAGPDGNVLELVPPYTITEQQLQHALDVLDACLKTAS"
     gene            complement(1548..2828)
                     /locus_tag="PLY56_06240"
     CDS             complement(1548..2828)
                     /locus_tag="PLY56_06240"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOJ21114.1"
                     /translation="MRERIEAGLADLRQARAESLAPLTVTERVELLDAIGRRLEEALA
                     GNERCPLAQSLQALLAREPVAEGTRAGIQAFATSLRRDTLWALLQEELGDPRVLDEFR
                     PRATGGWHRATAPEVTACFLAGNTPLLAWPPLAACLLAGSAVFGKLSRADTECPRLFQ
                     EAVAAVHPELGRCIALEVWPGGSPELDAALCAGADAVIAFGSDATIATLRARTPAGTP
                     FHGYGHRLSIALLLQGADEAAAAAGLARDVLLYDQQGCLSASMLFVEGDVPEAARFGE
                     RLARALEDQAVRWRLGPRDTEGCARVRVVRETALFAPNAHVWGDPGYRWSVVLAPGTR
                     PEPHGGECVVPIYPFTEMSDLDRLLRPLRGALQGAAVAGPSSRMPAAAEILARAGVSR
                     ICAPGTLQVPSFAWHHNGSSIVRHLLHWTDLETA"
     gene            complement(2821..3930)
                     /locus_tag="PLY56_06245"
     CDS             complement(2821..3930)
                     /locus_tag="PLY56_06245"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ21115.1"
                     /translation="MHHEAQEISGGSAEERRLFDALAAFIDSGDESDAGFDQHARALF
                     AYQYAHNEPYRRFCTARGVTPDRVTHWSQIPAAPAAAFKAFDLTCTPPEQTARVFRSS
                     GTTGRPSSRHYLSATALELYDRSLRAGFARFVLPDGARLPIWALVPPPETAPESSLSH
                     MLGSLMAQDPDLDHRFFWSDHGPDIPGLAGALAGVREPLILFGTAFAWLLFFDGQDGR
                     FALPEGTRVLETGGFKGRTREIPREELYALFTERLGVPPARCLAEYGMCELCSQFYDL
                     PPSSTGSGQETRRKQGPPWIRTRVVDPRTGEECPPGVPGVLCHYDLANLNSVLAVATE
                     DLGVRAGDGFELLGRVPGAELRGCSLTAEEWHARA"
     gene            4708..4989
                     /locus_tag="PLY56_06250"
     CDS             4708..4989
                     /locus_tag="PLY56_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOJ21116.1"
                     /translation="MESEILKVSGQSSPKMVAGAIASVVRRDGGVSVQAVGAGAVNQA
                     VKAIAIARDYVAPNGIDLVSVPSFFKIETDDGTKTGIRFLLEPRAPHEA"
     gene            5065..>5288
                     /locus_tag="PLY56_06255"
     CDS             5065..>5288
                     /locus_tag="PLY56_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ21117.1"
                     /translation="MHLKRLELFGFKTFADRTELEFLPGVTAVVGPNGSGKSNIADAI
                     QWVLGEQSMRSLRSLSAQDVIFAGTQRRRPL"
BASE COUNT          888 a         1772 c         1836 g          792 t
ORIGIN      
        1 ccgacctgga taatcaggcg attgctaccc atcccgaggt gcggcagctc ctgcgtgctg
       61 agatcgaccg gctcagcgac ggcctcgcgg agttcgagcg cgtgaaacgc tttgccgtgc
      121 tcgaccgcga attcagcatc gagttgggag agctgacgcc gtcgctcaaa ccgcgacggg
      181 gtgtcatcct ggagcggttc gcccctcagg tgaacagcct tttccggtcg tagccaatgg
      241 ctacgacgcg gtcttgaggc aggcgtccag cacgtccagc gcgtgctgga gttgctgctc
      301 ggtgatggtg tagggcggca ccagctccag gacattccca tcaggaccgg cgggcagcag
      361 gatgaccccg cggcgaagcg cctctacgac gacgcgcgcg gcggcgtggg tggccggcgt
      421 cccggaggca tcgcgtagct ccaggcccca catcagcccg cggccacgcg cctctgccac
      481 cagcgcgtgc ctctcgcaca gcgcctccag gcgcgggcgc agccactcgc ctagctgtcg
      541 gcaacgctcc accagcccac gcttctctat ctcggcgatg gcagccagcg cggcggcgca
      601 gcccaagggg tttccaagga aggtgctcgt gtgcagcgcc tcgccctgcg attcgcccca
      661 tgtcgccatg atctccggcg tgctgacgca cgcggagatc gggaagccac cggtcatcgc
      721 cttgcccacg cagaggatat ccggcacgac ttcgctggca aaccaggttc cggtgcgtcc
      781 gaagccggtg aagatctcgt cggcgatcag cagagcgccc cgctcatggc agagcgcgag
      841 gaggtcggag agccagccgg aaggcgggat gcgaatcccc ccgcggccct gaatcggctc
      901 cactaagacg gcgcccacgg ggggcaggtc cggcggtggg cagcgcagcc actccgcgac
      961 ctgctcgagc acgcgcgctg cctccgtggc atcgacgggg taggggaggt gccggacaaa
     1021 gcggcccagc tgctcgcgga aggggcggcg gaaatcgtcg cgccctgtgg ccgcgagagc
     1081 tccgtaggta agcccgtgat acgcgccctc aaaggcgatc accccggcgc ggcccgtggc
     1141 aaccgccgcc gtcttcagcg ccgcttcgat gctctcgccc ccggagctgc ccagaatcgc
     1201 ctgctccagg cgtccggggc agacgcgcgc caatgcacgc agcagctcca ccttcacggc
     1261 cggcgggtgg acatcgccca tgccatgcgg caggcggctg gcctgcgcct gaatcgcggt
     1321 aacgacgcgc gggttcgcat ggcctacggc ggcggctcca aaaccggcgc tgaggtcgag
     1381 gaaggtgttg ccgtcgacgt cggtcaccag gcaatcggac gcgctctccc agaagatggg
     1441 gaaatcgggg ctgacgtagg tgatgttcgg gctctcccag cggcgtagct cgcgagacat
     1501 ctcgcgcgag cgtggcccgg gcacctcggt tcgtagctca ggcagcatca ggcggtctcc
     1561 aggtcagtcc agtgcagcag gtggcgaacg atggaactcc cgttgtggtg ccaggcgaag
     1621 gatggcactt gcagagtgcc gggggcgcaa atccggctca ctccagcgcg cgccagaatc
     1681 tcggccgccg cgggcatgcg cgagctggga ccggcgacgg cggctccctg cagcgcgccg
     1741 cgcaggggcc ggagaaggcg gtccagatcc gacatctccg tgaacgggta gatcgggacg
     1801 acgcactccc ccccgtgcgg ctcaggccgc gtgcccggcg cgaggactac gctccagcgg
     1861 tagccgggat ctccccagac gtgggcattg ggcgcgaaaa gcgccgtctc gcgcacgacg
     1921 cgcacgcgcg cgcatccctc cgtgtcgcga ggacccagtc gccatcgaac cgcttggtcc
     1981 tccagcgccc gcgcgaggcg ctcgccaaac cgcgctgcct cgggcacgtc gccctcaaca
     2041 aaaagcatcg atgccgagag gcacccctgc tggtcgtaaa ggaggacatc gcgcgccagt
     2101 ccggcggctg cagcagcctc atcggcgcct tgcaagagga gcgcgatgct caagcgatgg
     2161 ccatagccgt ggaacggcgt tcccgccgga gtgcgcgcgc ggagggtggc gatggtggca
     2221 tcggatccga aggcgatcac ggcgtcggcg ccggcacaca gggcggcatc gagttcaggc
     2281 gagccgcccg gccagacctc cagggcgatg caccggccga gctccgggtg caccgcggcg
     2341 acggcctctt ggaaaagccg cgggcactcc gtatcggcgc gcgagagctt tccgaacacg
     2401 gcggagcccg cgaggaggca cgcggcgagc ggcggccagg cgaggagcgg cgtattcccg
     2461 gctaggaagc acgcggtgac ctccggagcg gtggcccggt gccatccccc agtggcgcga
     2521 gggcggaact cgtccagaac gcgcgggtcg cccagttcct cttggaggag cgcccagagc
     2581 gtatcgcggc gcaacgaggt ggcgaatgcc tggatgccag cgcgcgtgcc ctcggcgacc
     2641 ggctcccgcg cgaggagagc ctgcagtgac tgggccagtg ggcagcgttc attgcccgcc
     2701 agcgcctcct ccaggcgccg gccgatggcg tccaggagct ctactcgctc ggtgacggtc
     2761 agcggggcaa gagattccgc gcgagcctga cgcagatcgg ccaagcctgc ctcaattcgc
     2821 tcacgcacgg gcgtgccact cctccgcggt cagcgagcag ccgcgtagct ccgcgccggg
     2881 gacgcgcccc aacagttcga agccatcgcc agcgcgcacc cctaggtcct ctgtcgcgac
     2941 ggcgagcacg gagttcaagt tggcgagatc atagtggcag agcaccccgg gcacgcccgg
     3001 agggcactcc tcgccggtgc gcgggtccac gacccgggtg cggatccacg ggggaccctg
     3061 cttgcgccgg gtttcctggc cgctgccggt ggaagacggc ggaagatcgt agaactgtga
     3121 gcacaactcg cacatgccgt actcggccag gcagcgcgcc ggcggaaccc ccagacgctc
     3181 ggtgaagagt gcgtacagct cctcgcgcgg gatctcgcgc gtgcgtccct tgaagccgcc
     3241 cgtctcaagg acgcgcgttc cctctggcag ggcgaaccgc ccatcctgcc cgtcgaagaa
     3301 gaggagccac gcgaaggcgg tgccaaagag gatcagcggc tcgcgaacgc cggcaagcgc
     3361 cccggccagg cccggaatgt cggggccgtg gtcgctccag aaaaaacggt ggtccagatc
     3421 ggggtcctgc gccatgagcg agcccagcat atgcgagagc gacgactccg gggcggtttc
     3481 gggagggggc accagtgccc agatgggcag gcgcgcgcca tccgggagca cgaatcgcgc
     3541 gaagcccgcg cgcagcgagc ggtcgtacaa ctcgagcgcc gtggcggaga ggtagtgccg
     3601 gctggacggc cggcccgtcg tgccggagga acggaagact cgggcggtct gctccggagg
     3661 cgtgcatgtc agatcgaacg ccttgaacgc tgccgcgggt gccgcgggga tctgcgacca
     3721 gtgggtgacg cgatccggcg tgaccccgcg cgcggtgcag aaccggcggt acggctcgtt
     3781 atgggcgtac tgataggcga agagagcgcg cgcgtgctgg tcgaagccgg catcgctctc
     3841 atcgcccgaa tcgatgaacg ccgccagggc gtcaaagagg cgccgctcct cggcgctgcc
     3901 cccagagatc tcctgtgcct cgtgatgcat tcgtcctccc ggctcccatt ctaccacagg
     3961 ggggtgaccg tggcaactcg ccggccgcag tctcgaggtg gtcatctggc tctcgcagga
     4021 gacgcgaaac cgaggctgtt cgcaccctcc gcatccgtgt ctgtttccca catccgtggt
     4081 atcgccgccg gcgcgcgaaa aaggggcttg ccaaaaccgc gcatttctcc tataataggg
     4141 cggcaccggg aaccgggcgg atggtgggga aatgctcgac agaggagcgg tttccccttt
     4201 acttttgcct gggcactggg aacgttctct tttcgacgcc tggaccccaa agacgtccgg
     4261 agtagccccg agtttcgcca tcacggccgg ttcctcaccg gccggggcgc gggcaaattc
     4321 ggtggccgga ccgccgtcct caggtcacgc gtacgataac taacaaggat gggtgaagtt
     4381 ttagcgcgcc gcgaatcaac cgcattcagc accgaatcag cgagcgccgc ggctgtttgc
     4441 ccggcgggct gcctgtccgt atcgaccgga tgggcgagca gcaccctgct ccttctatcg
     4501 acctggttat gcaggcctcc atggtgcatg cccgggagta tccgcggaag ctctcagcgc
     4561 gcctgatccc atgcagcgca ggttgccgac tccgcttcgc catgctacag accttcgctg
     4621 aaacagccgt taccggtcat caagccttct cgccaccggc ccagtgccgc ggggaggcgt
     4681 tcgcactcaa ttgcaggagg cgagtgtatg gagagcgaga tcctgaaagt atctggacag
     4741 tcgtccccga agatggtcgc cggcgcgatc gcttcagtgg tccgccgtga tggcggtgtg
     4801 agcgtgcagg cggtcggcgc gggtgccgtc aaccaggccg tcaaggcgat cgcgatcgcg
     4861 cgcgactatg tggcgccgaa tggcattgac ctggtatcgg ttccctcttt cttcaaaatc
     4921 gagacggatg acggcaccaa gacggggatc cgtttcctcc tggagccgcg cgcaccgcat
     4981 gaagcgtgac gtggatcacg cgcgcgtcgc tgcttgttgt gggtggcccg cggtcccgga
     5041 gccaggcacg aggggttagt atgaatgcac ctcaaacggc ttgagttgtt cgggttcaag
     5101 actttcgcgg accggacgga gctggagttc cttccgggag tgaccgcggt ggtgggcccc
     5161 aatggcagcg gcaagagcaa catcgcagac gcgatccaat gggtcctggg cgagcagagt
     5221 atgcgctcgc tgcgcagtct ctccgcccag gacgtgatct ttgccggcac ccaacgccgg
     5281 cgccctct
//
LOCUS       DAOSCV010000198         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9848_length_5285_cov_2.986998, whole genome shotgun sequence.
ACCESSION   DAOSCV010000198 DAOSCV010000000
VERSION     DAOSCV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9848_length_5285_cov_2.986998"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLY56_06260"
     CDS             complement(<1..711)
                     /locus_tag="PLY56_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21118.1"
                     /translation="MKRMLRWRIRRCVASLLVLAMTFPLGEGLMARALAPAAQAAPSR
                     QRTIVVFEFDNKSKEGGEGLARALTRQIRLAMEQSPAFDLVSFSTTRPYNATIERAIQ
                     NGQLLEQELAGVPDSTTAVKIARILGAETALVGEIDADGYEYDANQNRVALRVTAYLY
                     NAATGEPTLTVTVNGEGKGEGLRGALEDRARMDVAKRLEEKRAGREPKEPRKPGARRK
                     WLPWALGGVALAVLAISLL"
     gene            complement(795..>5285)
                     /locus_tag="PLY56_06265"
     CDS             complement(795..>5285)
                     /locus_tag="PLY56_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOJ21119.1"
                     /translation="MYNRRWFQQPHFNLPKTDPASPPRQVFHPPFEIRVDPQITVDQV
                     QLRGDTGLGLIGIPVPVAALRDNGGGNKRRVALFGDDLNRARPSRLRSTDEIQWRFYY
                     TQSEGLPPTVCQMYLFRQDENGSFVPDPRSPIACTPLASGANGMDPLYKDFSDPTLSS
                     AFFAQYGVKLPGLAAPALEPGVYRYFFRVSDGRRTAEWPGDSDGGTSLTTRAQVSHIA
                     NTLRINHPPELSQHRAVPTVDPKTGAVSWTIEVVYRDLDGDPPSTPHLVFPDPSEPDN
                     AAKSLVLVMQRDPSTSDFTQGVRYRLQSATDPTVGAFQGRREYYFAFTDNWKDANNPE
                     DGEQVTLLDVDPNNPTGPGKPFFLNSKPFLLDVKVENATDPGKGAKGSLSDNFKFSVR
                     YGDNERDTPAGDLLELVIDGQDVHQMVRDPAQPNPDYAAGVIYTVTLNGRQIGAGAHS
                     FRIVAKDPLTNEEVQVQGTGPSIYVPTLSEPSVQRTDGTTEGSPSAGKPGTGFRYSVR
                     YRHEGDKPGDRVFVHILNPASGGQATVLDTVELKPEDPNPAPGTVAGQGVVWRNETPY
                     TFNAPGTYSYYFEASDGDSVRQFYADDANKVPFTGPVVGMPVLTPQAVTPLRSVVAQT
                     YEFSVLYSHAGSIEPQSVQLRIFDPKGALVDLPNQGAMTPPAGASRAALANPGWLFSL
                     PVKLDQPGVYRYGFTASDGVTTVETPVADGPTVMAIELPVLTGESVSPTPGQPGKVTY
                     RLTYRQSQNLVPSILVVAPNQDGNPESRPMVQENPADTDFTDGAVFAYTGASPAGSGP
                     WSGPYSYYFLASVPGIQDPIRYPNEGGAHGPNVVPTLMLDTEVGGSLPRLNPTTGHTG
                     TPFTFSVRCRDADAPANWKEAGLPRVSVIVGNQKLPMTYVEGDVRGKAGALFQTTTTL
                     PAGSLMHSFTAEDGFDTATLSDQNGRPFAGPMVLPETRLNLTLTPEQLTLDGLTPMRV
                     SGALDPVIPSTDIKITYAWVGADGQPVEMYVNTVTATSEGRFTDAYVPVRSGRWQVTA
                     EFESDAARGIGGGYEQVAVDVSPGEVSIPAGLSMIGVPVTPDQGQISDIFAGTDFQLA
                     RWNVALGRYDFGPGGTMPPVKPGDGFWVNTKGGIVARTVGETVPHNAAVSVPVQKGWN
                     QVANPFLLPISWSSVTFQDEQGTVMSLSEAVMAPPAERKIFDHAWIFDGSQYVLVHAT
                     VPGARRDVLPWQGLWVKSNVAGRLVFAPPMARSARLPEEPTRRRERGRTGEWTLQLVA
                     GNGVQSDSYNFAGVTSEGRALAISGLESPPPTLGQQIDLYFTGQAGTGRYATDFRPSA
                     SGLPLVFDFVVETNARDRAVSVRCPNLAELPKELDVILEDVDAAGKRVYLRTASGYSF
                     NPGDTGTRRLRLIVQPRTEGALRIDGITFGTTRGGRSISFSVSRASVATVEIIAPNGK
                     RLKKVADRRAVEQGMNTVTWDLTRADGSRLPRGLYLVQITATTPEGQTARAVRPIAVV
                     TW"
BASE COUNT          863 a         1816 c         1661 g          945 t
ORIGIN      
        1 cagaagcgag atcgccagca ccgccagggc aacaccaccg agagcccagg gcagccactt
       61 ccggcgcgct ccgggcttgc gaggctcctt cggctcgcgt cctgcgcgct tctcttccag
      121 gcgcttcgcc acgtccatgc gcgcccgatc ctcaagagcc ccgcgcaggc cctcgccctt
      181 gccctcgcca ttcaccgtga cggtgagcgt cggctcgccg gtcgctgcgt tgtagaggta
      241 cgccgttacc cgcaacgcca cgcggttctg attcgcgtcg tactcgtaac cgtcggcatc
      301 gatttcgccc acgagagccg tctcggcgcc gaggattcgc gcgatcttca ccgcggtggt
      361 ggaatcagga accccggcca gttcctgctc gaggagctgg ccattctgaa tcgctcgctc
      421 gatggtcgcg ttgtagggcc gtgttgttga gaagctaacg aggtcgaacg caggcgattg
      481 ctccatcgcc aagcggatct gccgggtcag cgcccgggcc agcccctcgc cgccctcttt
      541 gctcttgttg tcgaactcga aaacgacgat cgttcgctga cgcgagggag cggcctgcgc
      601 ggccggtgcc aacgccctgg ccatcaggcc ttcgcccaac gggaacgtca tcgccagtac
      661 caggagcgaa gcgacgcatc gcctgatccg ccacctaagc atgcgtttca tccagcctga
      721 actcccttcg cgctggcgcg tcggaagcgc ggccgccccg ggggaccgcc tggggcgggc
      781 cgccacttcc actctcacca ggtcaccact gctatcggtc tcactgctcg ggcggtctgc
      841 ccttcaggtg ttgttgccgt aatctgcacc agatacaagc cccgcggcag gcgagagcca
      901 tccgcgcggg tgaggtccca cgtcacggtg ttcatcccct gctccaccgc gcggcggtca
      961 gccaccttct tcaggcgctt gccgttcggc gcgatgatct ccaccgtcgc cacggaagcg
     1021 cggctcacgg agaaggagat gctccggccc cctcgggtcg tcccgaaggt aattccatcg
     1081 atgcgcagcg cgccctccgt gcgcggctgc acgatgaggc gaagccggcg cgtgccggta
     1141 tcccccgggt tgaaggagta gcccgatgct gtcctcagat agacgcgctt gcccgcggca
     1201 tccacatcct cgaggatgac atccagctcc ttgggcaact ccgcaaggtt ggggcaccgc
     1261 acactcacgg cgcgatcccg tgcattcgtc tccaccacga agtcgaacac tagtggcagc
     1321 ccactcgcgc tcgggcggaa gtcggtcgcg tagcggccgg ttccggcctg ccccgtgaag
     1381 tagagatcga tctgctggcc gagcgtcggc ggcgggctct ccagaccgga gattgccagc
     1441 gcgcggccct cgctcgtcac gccggcgaag ttatagctat cggactgaac gccatttccg
     1501 gcgaccagtt ggagcgtcca ctctcccgtg cgcccgcgct cgcggcgtct cgtcggttcc
     1561 tcgggcaggc gggcgctgcg cgccatcggc ggcgcgaaga ccagccggcc agccacgttc
     1621 gacttgaccc acaggccctg ccacggcaac acgtctctgc gcgcgccagg cacggttgca
     1681 tgcacgagca cgtactgcga gccgtcgaag atccatgcgt ggtcgaagat cttccgttcc
     1741 gccggaggcg ccatgaccgc ctccgagagc gacatcaccg tgccctgctc atcctggaac
     1801 gtcacgctgc tccaggagat cggcagtagg aacgggttcg ccacctggtt ccagcccttc
     1861 tgaacgggca cgctcacagc ggcgttgtgc ggaaccgtct ctcccaccgt cctcgcgacg
     1921 atgcctccct tggtgttcac ccagaagccg tcacccggct tgacgggcgg catcgtccca
     1981 cccgggccaa agtcatagcg acccagcgcc acgttccagc gcgcgagttg gaagtccgtc
     2041 cccgcaaaga tgtcgctgat ctgcccctgg tcgggcgtga ccggcacgcc gatcatcgac
     2101 agcccagccg gaatgctcac ttcgccaggg ctcacgtcta ccgccacctg ctcgtatccg
     2161 ccaccgatgc cgcgcgcggc gtcactctcg aactcggcgg tcacctgcca ccggccgctt
     2221 cggaccggaa cataggcatc cgtgaaccgg ccctcgctcg tcgcggtgac ggtgttcacg
     2281 tacatctcaa caggctggcc atccgcgccc acccacgcat aggtgatctt gatatccgtg
     2341 gagggaatca cgggatcgag cgcgccgctg acccgcatgg gggtcaaccc atcgagcgtc
     2401 aactgctccg gggtgagcgt caggttcagg cgtgtctcgg gaaggaccat gggtcctgcg
     2461 aacggcctgc cgttctgatc cgagagcgtg gcggtatcga acccgtcctc ggcggtgaag
     2521 ctatgcatca ggcttcctgc cggaagcgtg gttgtcgtct ggaacagtgc gcccgccttg
     2581 ccgcgaacgt cgccctcgac gtaggtcatc gggagcttct ggttgcccac gatgacggag
     2641 acgcgcggca gacccgcttc cttccagttt gcgggcgcgt cagcgtcgcg gcagcgcacg
     2701 ctaaaggtga acggagtacc cgtgtgtccc gtggtcgggt tcagcctcgg gaggctaccg
     2761 cccacctcgg tatccagcat gagcgtgggc accacgttcg ggccatgcgc accaccctca
     2821 ttcgggtagc gaatcgggtc ctggatgcca ggaacgctcg ccaggaagta gtagctgtac
     2881 ggcccactcc acggcccact ccccgccggg ctcgcaccgg tgtacgcgaa caccgcgcca
     2941 tcagtgaagt ccgtatccgc cgggttctcc tgcaccatcg gccggctttc ggggttgccg
     3001 tcctggttcg gggcgaccac gaggatggag ggcaccaggt tttggctctg gcgataggta
     3061 agccggtacg tcaccttgcc aggctggccg ggcgtcggcg agacgctctc ccctgtcagc
     3121 accggcagct cgatcgccat cacggtaggc ccgtcagcaa ccggcgtctc cacggtagtc
     3181 acgccgtcgc tcgccgtaaa tccataccga tagacgccgg gctgatccag cttcaccggc
     3241 agggagaaga gccacccggg attcgccagg gccgccctgg aagcacctgc tggcggggtc
     3301 atcgcgccct gattgggcag atccacgagc gcgcccttcg ggtcgaagat tcggagctgc
     3361 acgctctgtg gttcgatgct ccccgcgtgc gagtacagaa cgctgaactc gtacgtctgc
     3421 gccaccactg aacgcagagg cgtcactgcc tgcggcgtga gcaccggcat gccgaccacc
     3481 gggcccgtga atggcacctt attcgcgtcg tcggcataga actggcgcac gctgtcgcca
     3541 tccgacgcct cgaagtagta gctgtaggtg cccggtgcgt tgaacgtgta cggcgtctcg
     3601 ttcctccaca ccaccccctg ccccgcaacc gtcccaggag ctgggttcgg atcctccggt
     3661 ttgagctcca ccgtgtcaag cacggtcgcc tggccgccac tcgcggggtt caggatgtgc
     3721 acgaacaccc tatcgccggg cttgtcgccc tcatgtcggt agcgcacgct gtagcggaag
     3781 ccggtcccgg gcttacccgc gctgggagaa ccctccgtgg tgccatccgt gcgctggacg
     3841 ctgggctcgc tcagagtcgg cacatagatg ctcggcccgg tcccctggac ctgcacctct
     3901 tcgttggtca acggatcttt cgctacgatc ctgaaggagt gcgcccctgc cccgatctgc
     3961 cggccgttga gcgtgacggt atagatgaca ccggctgcgt aatccggatt cggttgggcc
     4021 ggatcgcgca ccatctgatg gacgtcctgg ccgtcgatca cgagctccag gaggtcgccc
     4081 gcgggcgtgt cacgctcgtt atcgccgtac ctcacgctga acttgaagtt gtcgctcagc
     4141 gaacccttgg ctcccttgcc gggatccgtg gcgttctcga ccttgacatc gagcaagaac
     4201 ggcttgctat tcaggaagaa cggcttccca ggcccagttg ggttgttggg gtccacatcc
     4261 aacagggtga cctgctcacc atcctccggg ttgttggcgt ccttccagtt atcggtgaac
     4321 gcgaagtagt attcgcgtcg gccctggaac gcgcccacgg tggggtcggt cgccgattgc
     4381 aggcggtagc gcaccccctg ggtgaagtcc gacgtgctcg gatcccgctg catgacgagg
     4441 accaggctct tcgcagcatt gtccggctca ctgggatccg ggaaaacaag gtgcggcgtg
     4501 ctggggggat cgccatcgag atcccgatac accacctcaa tggtccagct cacggcgccc
     4561 gttttcggat cgacggtcgg caccgcgcgg tgctgcgata gctccggcgg gtggttgata
     4621 cgaagcgtat tcgcgatatg gctcacctgc gcgcgcgtgg tgagcgaggt gccgccatcg
     4681 gaatcgcccg gccattccgc cgttcgccgg ccatcgctga cgcggaagaa gtagcggtag
     4741 acgcccggct caagcgcggg cgccgccaga cccggtaact tcacgccata ctgcgcgaag
     4801 aacgcagacg agagcgtggg atcagagaag tccttgtaga gcgggtccat gccgtttgcc
     4861 ccgcttgcaa gcggtgtgca cgcaatgggg gaccgtgggt cgggcacgaa gctcccattc
     4921 tcatcctggc ggaagaggta catctggcac acggtcggcg gcaggccctc ggactgcgtg
     4981 taatagaacc gccactgaat ctcatccgtg gagcgaagcc gactggggcg cgcgcgattc
     5041 aggtcatctc cgaagagagc cacacgcctc ttgttgccac cgccattgtc acggagcgcc
     5101 gccaccggca ccggaatccc gatcagcccg aggccagtat cccctcgcaa ctgtacctga
     5161 tccaccgtga tctggggatc cacgcggatc tcaaacggcg ggtggaacac ctggcgcggg
     5221 ggcgaggctg ggtccgtttt cggcaggtta aagtgcggct gctggaacca gcgccggttg
     5281 tacat
//
LOCUS       DAOSCV010000199         5264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9893_length_5264_cov_3.660587, whole genome shotgun sequence.
ACCESSION   DAOSCV010000199 DAOSCV010000000
VERSION     DAOSCV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9893_length_5264_cov_3.660587"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PLY56_06270"
     CDS             <1..974
                     /locus_tag="PLY56_06270"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21120.1"
                     /translation="LGLEGHAHIGKPYTEVIANDQVKEMLRLALAANQEVSQAELTLT
                     FVDPGSGQEHEAAFQIQTALVRGTDSGDLIGVALVFNDISEICSLERMKSAFLSTISH
                     ELNTPLTTIRGFISTLREDTKGFYDLKTRQEFYQIVDAECERLSRLIGDLLSLSRIQS
                     TGRLELTLAPVDIAALVERVVETQRAYVSKHTLRADLFPGLPPILGDEAKIEQVLVNL
                     VSNAIKFSPGGGEVLVQVTPEDDGIRVSVRDHGVGIPREELPRIFERFHRLDNRDKRQ
                     TYGCGIGLYLVKHLVEAHGGSVSVESEVEKGSLFSFCLPLRPPDAEE"
     gene            complement(1039..1953)
                     /locus_tag="PLY56_06275"
     CDS             complement(1039..1953)
                     /locus_tag="PLY56_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ21121.1"
                     /translation="MSDHWDLLGLGAVAVDDLLYVEHYPQPETKTPVHDRRREGGGLA
                     GTALAAAARQGAKAAYFGVLGDDELSRFTLREFERDGVDCSMVIRRAGARPIHSTVII
                     EASTGRRTILFTRAGFTAPQPDEIPASLVARCRILFLDHTVAEAGVRAASIAREHGIP
                     VLADLERDTGPKLAELLPLIDHLVLGIRFGSHLTGEEDPVAITRALACPARRSCVVTA
                     GERGCWYSIAGGEVRHLPAFPVRVVDTNGCGDVFHGAYAAQLARGERIDAAVRFASAA
                     AAIKATRPGGRSGIPQHGEVERFLAERG"
     gene            2269..2898
                     /locus_tag="PLY56_06280"
     CDS             2269..2898
                     /locus_tag="PLY56_06280"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ21122.1"
                     /translation="MTYTILARWIGVWILLWVGFAAPAEARKEIVVDYEAGLVYPLEN
                     GRLVRGPIITNHGVVFKKKYLGTFRVTEKLIEKRSNLYNRHGQPIRNGEEGARMPYWM
                     RLGHTAQGFHYSSLFSATGPRRRSRGCYRLSKADAIWLFNWTPIGTPVHVVWSVKQSR
                     FAYLQKATSSRRASLPLEPQGVSVRPALPMRTVAQGVAHPGLPARRYHG"
     gene            3312..4403
                     /locus_tag="PLY56_06285"
     CDS             3312..4403
                     /locus_tag="PLY56_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HOJ21123.1"
                     /translation="MPKLFGKQFSKEELRTHVGSMEQLAGVRAGVLADGRAAGTRVAD
                     FWTGTGLEFSVLLDRGMDISAARWQGKSLCWRSSAGVAHPAYYEPEGLGWLRTFGGGL
                     VTTCGLTYAGAPTVDQGEELGLHGRVSHLPADSVHLDAEWDGDDYVIWASGKVREARV
                     FGENIVLSRKIFTRLGENRFWIHDRVENEGYQTTEHMLLYHINLGFPVVDEGSVLLAP
                     SRSVQPRDAEAEKEREAYAEFHAPRPGYQEKVYYHDLAAGADGMVSAAVVNRDTDAGT
                     GFGVYVAYSRNELPYLVEWKMMGQGTYVVGMEPSNCLVGGRDQERAAGRLQFLEPGEC
                     REYRLEIGVLSSLLEITAFEEQVQALLGK"
     gene            4440..4994
                     /locus_tag="PLY56_06290"
     CDS             4440..4994
                     /locus_tag="PLY56_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21124.1"
                     /translation="MEKKRTAGAMAGAALVAAVVGEMLRRSGYSVRVALKRVIHADKE
                     AIIDLVRQVDREVQLIPAIRSVSILESSPEGVRYRVEGTSPLGAWWAEYHKWWDYEAG
                     TIGWASDKGAFGLRQRGRMNLEPVPDGTEVTLTTEYTSIAPVVGPAIAAAGKTLMVQP
                     NFQAWLGNIAAIVEKKEEQEPATS"
BASE COUNT          914 a         1596 c         1814 g          940 t
ORIGIN      
        1 tgttggggct cgagggccat gcgcatatcg gcaagcccta cacggaggtg atcgccaacg
       61 accaggtcaa ggagatgctg cgcctcgcgc tcgcagccaa ccaagaggtc tctcaggcgg
      121 agttgacgct caccttcgtt gatccgggca gcgggcaaga gcacgaggcc gcctttcaga
      181 tccagacggc gctcgtccgg ggcaccgata gcggcgacct gatcggcgtg gcgttggtgt
      241 tcaacgacat ctccgagatc tgtagcctcg aacggatgaa atcggctttc ctctccacca
      301 tctctcacga gctgaacacg cccctcacca cgatccgagg cttcatctca accctccgcg
      361 aggataccaa ggggttctac gatctgaaga cacgtcagga gttctatcag atcgtggatg
      421 ccgagtgcga gcggctctcg cgcctgattg gcgatctcct cagcctctcg cggattcaat
      481 ccacggggcg gctagagttg acgctcgcgc cggtggatat cgccgcgctg gtggagcgcg
      541 tggtggagac gcaacgcgcg tatgtgtcca agcacaccct gcgcgccgat ctcttcccag
      601 ggcttcctcc tattctcgga gacgaggcaa agatcgagca ggtgctcgtc aacctcgtca
      661 gcaacgcgat caaattctca cctggcggag gcgaggtcct ggtccaggtc acccccgaag
      721 acgatgggat tcgcgtctcg gtgcgcgacc atggagtggg catcccgcgc gaggagttgc
      781 cgaggatttt cgaacgtttt caccgcctgg ataaccgcga caagcgccag acgtatggat
      841 gcggaatcgg cctctatctg gtgaagcacc tggtggaagc gcacggaggg agtgtctctg
      901 tggagagcga ggtggagaag gggagcctct tcagtttctg cctcccgttg cgcccgccgg
      961 acgccgagga gtaggccgca gggccgtggc ggaactcgcc gcgcccttgc cttcgcccgt
     1021 cgccaccacg ggttgcggtc accctctctc ggccaggaag cgttccacct cgccgtgttg
     1081 cgggattccc gatcttcccc cgggccgcgt ggccttgatc gcggctgccg cgcttgcgaa
     1141 gcgcaccgcc gcatcgatcc tctcgcctcg tgcaagctgc gccgcgtagg ccccatgaaa
     1201 cacgtcgccg cacccgttgg tatcgacgac gcgcaccggg aacgccggaa ggtggcgtac
     1261 ctccccgccg gcgatggagt accagcaccc gcgctctccc gccgtcacca cgcaacttct
     1321 gcgcgccggg caagcgagcg cgcgcgtgat cgcgacagga tcctcttcgc ccgtcaggtg
     1381 tgacccaaag cggatcccca gaaccaggtg atcgatcagg gggagtagct ccgccagctt
     1441 cgggccggta tcgcgctcga ggtcggcgag tacgggaatg ccatgctccc gggcgatgct
     1501 cgccgcgcgc actcccgcct cggctaccgt gtggtcgagg aagaggatgc ggcatcgagc
     1561 gacgagcgat gcggggatct catctggctg tggcgcggtg aatccggcgc gcgtgaagag
     1621 aatggtgcgt cgcccggtag aggcctcgat gatcactgtg gagtggatgg gccgggcgcc
     1681 agcacgccgg atcaccatcg agcaatcgac gccgtcgcgc tcgaactcgc gcagtgtgaa
     1741 gcgagagagt tcatcatccc ccaaaacacc gaaataggcg gctttcgcgc cctggcgggc
     1801 tgcggccgcc agcgcggtgc cggccaaccc gcctccttcc cggcgcctgt catgcaccgg
     1861 tgtcttcgtt tccggctgcg gatagtgctc cacatagagc aggtcgtcaa cggcgactgc
     1921 ccccagaccg aggagatccc agtggtcgct catgcgccgc tccgcgctgt ctcctggaaa
     1981 aggggcgccc ctgcgtgggt cgggcgaacg aggatgcgca tcttgatgaa ctctccaaat
     2041 cggtagtggc cccggcgaca tcgcggatgc gagcgcggta cgatggggct cgctcacttc
     2101 gttcgacgcg ctgctccggg ctacctttcg gtccgagccg gggatcgcgg gccggcagag
     2161 cgatctcggg catcctccag tgggttgcga cgatcctggc atgagtcgcc gtgggcgggg
     2221 gtaggtgcag tgtatgctgc cgatgtgaca agatgtgttg gaggaggcat gacatacacg
     2281 attctggcca ggtggatcgg cgtgtggatc ttgttgtggg tgggcttcgc ggcgccggcg
     2341 gaggcgcgga aggagatcgt ggtcgactac gaggcagggc tagtctaccc gctggaaaac
     2401 gggcggttgg ttcggggccc gatcatcaca aaccacggag tcgtcttcaa gaagaagtac
     2461 ttgggtacct ttcgtgtcac cgaaaagctg attgagaagc gctcgaacct gtacaaccgg
     2521 catgggcagc cgatccgcaa cggtgaggaa ggggcgcgca tgccgtattg gatgcgcctc
     2581 gggcacactg cccagggctt ccattactcc agccttttta gcgcgaccgg gccgcgccgg
     2641 cgctcccgag ggtgttaccg cctctccaaa gccgacgcga tatggctctt caactggacg
     2701 cccatcggca ctcccgtgca cgtggtatgg agcgtgaagc agtcgcggtt tgcctacctg
     2761 cagaaggcaa cctcctcgag gagagcgagc ctgcccctgg aaccgcaggg agtgtcggtg
     2821 cggccggcac tcccgatgag aactgtggcc cagggtgtgg cgcatccggg cctgccagcg
     2881 cggcggtacc acggatgaag gtgacggata cggatgggga tatgcgggca cccagcccgg
     2941 tttgccgccc tttaggatgg ggggctcttg ccggaacact agccggggtg cccgcaccct
     3001 gggctgagtt atccgacccc ttcgtggccg tagcatgggc gctcttcggg ttcagggtgt
     3061 agctcggcat ccactcgtgg ccagagcgtt gcccttgaca cgccctctgc cctatggtac
     3121 aataacgcgc gacgaagcag cagggatgac ggagtaggtc gcggcggctc cgccctggtg
     3181 tgtcggaccc caagcccgcg cgagcggtga ttcctcggcc gtcgcttcga ctcctcgcct
     3241 ttcccgtgta ctctggcccg gtgatcctgc cagggcggag gcacgcgggt gaccaactcg
     3301 tgaggtgcac gatgccaaaa ctcttcggca agcagttcag caaggaggag cttcggacgc
     3361 acgtggggag catggagcag ctcgccggcg ttcgagcggg ggtgctggcg gatggccgcg
     3421 ctgcgggtac gcgcgtggcc gatttctgga ctggcaccgg cctggagttc agcgtgctct
     3481 tggaccgcgg gatggatatc tccgcagcgc gctggcaggg caagtcgctc tgctggaggt
     3541 cctccgctgg cgtagcccat cccgcctatt atgagccaga gggactggga tggctgcgca
     3601 cgttcggtgg tggcctggtg accacctgcg gcctgaccta tgctggtgca cccacggtgg
     3661 atcagggcga ggaactgggg cttcatgggc gcgtctcgca ccttcccgcc gacagcgtgc
     3721 atctggatgc ggagtgggat ggcgacgact acgtcatctg ggcgtccggc aaggtgcgcg
     3781 aggcgcgcgt gtttggcgag aacatcgttc tctcccggaa gatcttcact cgcctgggcg
     3841 aaaaccgctt ctggatccat gaccgcgtcg agaacgaggg ctaccagacg accgagcata
     3901 tgcttctgta tcacatcaac ctgggcttcc ccgtggtgga tgagggctcg gtgctcctcg
     3961 cgccgtcgcg cagcgtgcag ccgcgcgacg cggaggcgga gaaggagcgc gaggcatacg
     4021 ccgagtttca cgcgccgcgg cccgggtatc aggagaaggt gtactatcac gacctggcgg
     4081 ccggcgccga tggcatggtt tcggcggccg tcgtgaaccg agacaccgac gcgggcaccg
     4141 ggtttggcgt ctacgtcgct tattcgcgca acgaactgcc gtatctcgtt gagtggaaga
     4201 tgatggggca gggcacctac gtggtgggca tggagccttc caactgcctc gtgggcgggc
     4261 gcgatcagga gcgcgcggcg ggcaggctcc aattcctcga accgggagaa tgcagggaat
     4321 accggttgga gattggggtg ctctcgtcgc tgcttgagat cacggccttc gaggagcagg
     4381 tgcaggctct gctggggaag tagattctgg ggcagcgcct gcctcggaag gaggagcgga
     4441 tggagaagaa gcggacggct ggggcgatgg cgggagctgc cctggtcgcc gccgtcgtcg
     4501 gagagatgct ccggcgcagc ggttactccg tgagagtcgc tctcaaacgc gtgatccatg
     4561 ccgataaaga ggcgatcatc gacctcgtac gccaggtaga ccgcgaggtg cagctcatcc
     4621 ctgcgatccg gagcgtctcc attctggaga gctctcccga gggggtgcgc tatcgcgtgg
     4681 aggggaccag ccccttgggc gcatggtggg cagagtacca caagtggtgg gactacgagg
     4741 ccggcaccat cggttgggcc agtgataagg gtgcgttcgg gttgcggcaa cgcgggcgta
     4801 tgaacctgga gccggtgccc gatggcaccg aggtgaccct tacaaccgag tatacctcca
     4861 tcgccccggt cgttggaccc gcgattgccg ccgcggggaa gaccctcatg gtgcagccga
     4921 acttccaggc atggctcgga aatatcgccg ccatcgtgga gaagaaggaa gagcaggagc
     4981 cggccacctc ttgagcacgg agttgagcac gaggtggggc tctctatctc gcctcgtgcc
     5041 ctctatcctg ccgggcgagt ggcaagatct ttgcgccagc ggaaaaatgg cgtataatag
     5101 ttagtaggta gaccgggtgc aatcatgcag cccggcagtt cggtgaacgc ggtgcctgga
     5161 aggtgacacg agatgttgaa tagcgtcaaa acggcggtgc tgctggcggc gctcaccggg
     5221 atactggttg ccatcggcaa tgcgctgggc gggccagaag gcgc
//
LOCUS       DAOSCV010000200         5258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9908_length_5258_cov_2.909667, whole genome shotgun sequence.
ACCESSION   DAOSCV010000200 DAOSCV010000000
VERSION     DAOSCV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_5258_cov_2.909667"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLY56_06295"
     CDS             <1..192
                     /locus_tag="PLY56_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21125.1"
                     /translation="EGAREAMARVEQCRPFVMETPIQGCLRFPDKSTADGFQPRRSTR
                     VDDYTFEAVFEDARFIHEF"
     assembly_gap    259..268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            442..1806
                     /gene="der"
                     /locus_tag="PLY56_06300"
     CDS             442..1806
                     /gene="der"
                     /locus_tag="PLY56_06300"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019419171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ21126.1"
                     /translation="MSKAVVAVVVRPNVGKSTLFNRVVGRREAVVENIPGVTRDRLYG
                     IAEWNGREFTIVDTGGLVLDESDPMAARIRAQAELAIEEADVIVMVVDGTDGLTPLDE
                     DVAKSLRPANKPVLVAVNKTEGKKGKAGVPDFYALGIGELFPISATQGDGVADLLDEV
                     VARLPPPEQAEEFPEDVLRVALIGRPNVGKSSMLNAILGEERVIVSNVPGTTRDAVDT
                     YFERGGQKYVLVDTAGIRRPGKVQGSIEYYCVLRASRAIESCDVAIVVINGEEGLVDG
                     DKRVAGMAHEEGRACVIAVNKWDLMRKGHAKWTEQLGRRIRREFTEHVRAEMPFLDYA
                     PIVFVSALMGENIDDLLDTVNDVAESHARRVPTGELNRVIQDAVDANPRTEKGRTLRI
                     YYATMSEVSPPTIILFVNDPELMHFSYERYLINRLRQVFGYEGTPIRLITRRRTKEMA
                     RQTG"
     gene            1817..2350
                     /gene="amrA"
                     /locus_tag="PLY56_06305"
     CDS             1817..2350
                     /gene="amrA"
                     /locus_tag="PLY56_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOJ21127.1"
                     /translation="MADTQATGAHTPPVILARNAIIAYVTEGRVLDVPADLPLLLQQP
                     SGCFVSLKKHGALRGCIGTITPVHPSLAEEIIHNGIAACRDPRFPHPVSRDELGDLVI
                     TVDVLEPPEPVAGLADFDPKRYGMILRAGSRRGLLLPDLEGVETPEEQYEIVCRKAGI
                     FPGEAVEFSRFKSTRYH"
     gene            2418..2798
                     /locus_tag="PLY56_06310"
     CDS             2418..2798
                     /locus_tag="PLY56_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21128.1"
                     /translation="MRTMLDKVLDLAFGAADMTRERAQAIVDELIRRGEATREQGNTM
                     VEDLLRRGQQQREELRALIRREVSDTMSHMNMATRDDTARLERRIQELETLVEDLRGA
                     APAGSAEATAVASPEIAPAAETTE"
     gene            2795..3397
                     /locus_tag="PLY56_06315"
     CDS             2795..3397
                     /locus_tag="PLY56_06315"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOJ21129.1"
                     /translation="MRRRIILASASPRRRELLSQVGIPFEVIPADVPEQGRPETDPCE
                     LARTLALEKAQATAARIDDGLVLGADTIVVLDSRILGKPADAEDARAMLRALSGCTHQ
                     VITGVVLIDRQHGETVATDVAHAVTDVTFLPWSDEAIDAYVATGEPLDKAGAYAIQGY
                     AAVLIEGICGCYFNVVGLPLAVVARMLRARGVPVNPAPLA"
     gene            3514..4503
                     /locus_tag="PLY56_06320"
     CDS             3514..4503
                     /locus_tag="PLY56_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21130.1"
                     /translation="MTTDTALRWLGGVPLFDPSQGSTVIASPGQGPGWWVGAPSALYD
                     RESGPTYLYYRVRKPRALGRGGECRIAASRDGLNFETVWSAKKDAFGSPSIERGCLVK
                     TPEGRWRLYLSFVDPADDRWRIDLLEADTPEALDPGERRKILTAADIGGEGVKDPVVY
                     LLGGLYWMVVSYAPGRPDATAAEKAQMHATADVYNTGIVKSHTGLALSPDGVHFRWEG
                     DLLSPPDTGWDRYCTRISTLLYTPPVWTALYDGSGSVEENYEERAGVAVSLDGCHFER
                     LTLNGPALISPHASGSVRYVEALRLDTEIRYYYEFALPDGSHELRCSRVPLSG"
     gene            4585..>5258
                     /locus_tag="PLY56_06325"
     CDS             4585..>5258
                     /locus_tag="PLY56_06325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOJ21131.1"
                     /translation="MKRIGVLTSGGDASGMNACIRSVVRTAISRNVEVIGIRRGYDGL
                     IRGDVFPMDAASVGGILNQGGTILLTARSKEFMTREGQEKAVATLKRHGIEGLIVIGG
                     EGSFHGARVLGTEWGVPVVGVPATIDNDIAGTDFSIGFDTAVNVALEAIDKIRDTAAS
                     HERIFVIEVMGRSVGFIALEVGLAGGAEAILIPEVPYDLDTVCKRIASGRARGKISSI
                     VVVAEGA"
BASE COUNT          895 a         1661 c         1783 g          909 t
ORIGIN      
        1 gaaggcgcgc gcgaggcgat ggcgcgcgtc gagcagtgcc gtcccttcgt catggagacc
       61 cctatccagg gctgcctccg cttccccgac aagagcaccg cggatggttt ccagcccagg
      121 cgatccacgc gtgtggatga ctataccttc gaagccgtct tcgaggatgc gcgtttcatc
      181 cacgagttct gatagccacc gtcccttctt ccgcgcgcct gcccatcaca cggcgatggg
      241 acgggcaatt gttcagctnn nnnnnnnngg gctggccatc cgattggtcg tggggtgcgc
      301 ctgcgagccg ctccaatagg ctggaaaagc cggccctggc tggccgctgc atcctcgcgg
      361 acgcggaagc gtctcctccg ggcgatgaga aacccggttt ttccgttgac tccacccccc
      421 cctttgtgct accatgtctg tatgagtaag gcggtagtcg cagtcgtcgt gcggcccaac
      481 gtgggcaaat cgaccctgtt caaccgggtg gtggggcggc gtgaggccgt agtggagaac
      541 ataccgggcg tcacgcgtga ccgcctctat gggatcgcgg agtggaatgg ccgcgagttc
      601 accatcgtgg ataccggcgg cctcgtcctg gatgagagcg atccgatggc ggcgcgcatc
      661 cgcgcccagg cggagcttgc catcgaggaa gccgatgtca ttgtgatggt ggtcgatggc
      721 accgatgggc tgacaccact cgatgaggat gtggcgaaga gcctgcgtcc ggcgaacaag
      781 ccggtgctgg tcgctgtcaa caagacggag gggaagaagg gtaaggcagg cgttcccgac
      841 ttctatgccc ttgggatcgg cgaactcttc cccatctcgg ccactcaggg ggatggcgtg
      901 gccgacctcc ttgacgaggt ggtggccagg cttccaccgc cggagcaggc ggaggagttt
      961 cccgaggatg tgttgcgcgt tgccctcatc ggccggccca acgtgggcaa gtcgtcgatg
     1021 ctgaacgcga tcctcggcga agagcgcgtc atcgtcagca acgtacctgg gacgacccgc
     1081 gacgcggtag atacctattt cgaacgcggg ggccagaagt atgtgctggt ggacaccgcc
     1141 gggatccgcc gccccggaaa ggtgcagggc tcgatcgagt actactgcgt gctccgcgcc
     1201 agccgggcta tcgagagctg tgacgtcgcg atagtcgtca tcaatggcga agagggcctg
     1261 gtcgatggcg ataagcgcgt ggccggcatg gcccatgagg aaggccgggc gtgcgtcatc
     1321 gcggtcaata agtgggacct gatgcgcaag ggccacgcca agtggaccga gcaactcggc
     1381 cggcgcatcc ggcgggagtt caccgagcac gttcgcgcgg agatgccctt cctcgactat
     1441 gcgcccatcg tcttcgtctc cgcccttatg ggcgagaata tcgatgacct gctcgacacg
     1501 gtgaacgatg ttgccgagtc gcatgcccgg cgggtgccta cgggggagct gaaccgggtg
     1561 atccaggatg cggtggacgc caatccccgc acagagaagg ggcgcacgct gcgtatctac
     1621 tacgccacga tgagtgaggt cagcccgccc acgatcatcc tcttcgtcaa cgacccggag
     1681 ctgatgcact tctcttacga gcggtatctc atcaaccggc tgcgccaggt attcggctac
     1741 gagggcacgc cgatccgcct catcacgcgc cgccgcacga aggagatggc ccgccagacc
     1801 ggctaaggag gcagcgatgg ctgataccca agcgactgga gcgcatactc ccccggtgat
     1861 actggcgcga aacgccatca tcgcctatgt caccgaaggg cgcgttcttg atgttccggc
     1921 cgatctgccg cttctgcttc agcagccatc ggggtgcttc gtctcgctga agaagcacgg
     1981 cgccctgcgg ggctgtatcg gcaccatcac gccggtccat cccagtctgg cggaggagat
     2041 catccacaac gggatcgccg cgtgtcgtga cccgcgcttt ccccaccccg tctcgcgcga
     2101 cgagctgggc gacctggtga tcaccgtgga tgtgctcgag ccgcccgagc ctgtggccgg
     2161 gctggcagat tttgatccga agcgctacgg catgatcttg cgcgcgggca gccggcgggg
     2221 ccttctgcta ccggatctgg agggggtcga gacgcccgag gagcagtacg agatcgtctg
     2281 tcggaaggca ggcatctttc ctggcgaagc agtagagttc tcccgcttca agtcgacgag
     2341 gtatcactga gatcggcctc gacgggccag gtgcccattc gcccgccggg gaacgtgccg
     2401 tctgagagga gagcgcgatg cgcacaatgc tggataaagt cctggacctg gcgttcggcg
     2461 cagcggatat gacgcgcgag cgagcgcagg cgattgtgga tgagctgatc agacgtggcg
     2521 aggcgacacg cgagcagggc aacacgatgg tggaggatct gctgcgccga gggcagcaac
     2581 agcgcgagga gttgcgggcc ctcattcgcc gtgaggtgag cgataccatg tcgcacatga
     2641 acatggccac gcgcgacgat accgcccgcc tggaacgccg cattcaggaa ctggagacgc
     2701 tcgtggaaga cctgcgtggc gccgcgcccg ccggttcggc ggaggcaacc gccgtagcct
     2761 ctcctgagat cgctccggca gccgagacca ccgagtgaga cggcggatca ttctggcatc
     2821 ggcgtcgccg cgccggcgcg agcttctctc gcaggttggc atcccgttcg aggtgatccc
     2881 ggcggatgtg cccgagcagg gccggccgga gacagatcct tgcgagctgg cgcgcacgct
     2941 ggccctcgaa aaggctcaag ccacggccgc ccgcattgac gatggcctcg tcctgggcgc
     3001 cgataccatc gtcgtcctcg attcgcgcat ccttggcaag cccgccgatg ccgaggatgc
     3061 gcgagcgatg ctccgcgcgc tttccggatg cacgcatcag gtgatcaccg gcgtcgtcct
     3121 catcgaccgg caacacggcg aaacggtcgc caccgacgtc gcgcacgcgg tcaccgacgt
     3181 cacctttctc ccctggagcg acgaggcgat tgacgcctac gtggccacgg gcgagcccct
     3241 cgataaggcg ggcgcctacg cgatccaagg ctatgccgcc gtcctcatcg aaggcatctg
     3301 cggctgctac ttcaatgtgg tcggcctgcc cctggcggtt gtcgctcgca tgctccgcgc
     3361 ccggggcgtg cctgtcaacc cggcgccgct tgcctagcgc atggagtgca tcgatacctg
     3421 actgccgcat cggccacgcg ggcagagccc gaggcccgca cgcgctcttg gagcacggca
     3481 gcagcgggca tcggccagaa cggaaggagt gcgatgacaa cggatacggc actgcgctgg
     3541 ctgggcggag tgcccctctt cgacccgagc cagggctcca ccgtgatcgc gtctcccggg
     3601 cagggaccag gctggtgggt tggagccccc agcgcgctct acgaccggga gtctggaccc
     3661 acctacctct attaccgggt gcgcaagccc cgcgcgcttg gccgcggtgg cgagtgtcgc
     3721 atcgccgcga gccgcgacgg cctgaacttc gaaacggttt ggagcgccaa gaaggatgcg
     3781 tttggatcgc cttccatcga gcgtggttgt ctggtgaaga cgcccgaagg ccggtggcgg
     3841 ctctatctct ccttcgttga ccccgcggat gatcggtggc ggatcgacct gttggaggcg
     3901 gatacgccgg aggcgttgga cccaggagag cgccggaaga tcctcaccgc ggcggatatt
     3961 ggcggcgagg gcgtgaagga tccggtcgtc tacctcctcg gcgggctcta ctggatggtc
     4021 gtgagctacg ctcccggccg gccggacgcg acggccgcgg agaaggcaca gatgcatgcc
     4081 accgccgatg tctacaacac gggcatcgtg aagtcgcaca ccgggctggc cctcagcccc
     4141 gatggtgtcc acttccgttg ggaaggcgac ctcctctccc cgcctgatac gggctgggat
     4201 cgctactgca cccggatcag cacgttgctc tatacgccgc cggtctggac cgccctctat
     4261 gatgggagcg gctcggtgga ggagaattac gaagagcgcg ccggcgtcgc ggtcagcctg
     4321 gatggctgcc atttcgagcg gctgaccctc aatgggccgg cgttgatctc gccccacgcc
     4381 tccggaagcg tgcgttatgt cgaggcgctc cggttggata cggagatccg ctactactac
     4441 gagttcgccc tcccggatgg ctcccacgag ctgcgctgca gccgggtgcc gctttcggga
     4501 taggcccatc tgtctggatt cgcctgtcgg gcgcggattg gtcagcgccg gacaggcgct
     4561 gacgagcact aacttactgc cactatgaag agaatcggag tcttgacgag cggaggcgat
     4621 gcctccggga tgaacgcgtg tatacgctcg gtggtacgca ccgccatcag tcgtaacgtg
     4681 gaggtcatcg gcatccgccg aggatatgat ggcctgattc ggggcgacgt gttcccgatg
     4741 gatgccgcct cggtgggcgg catcttgaat cagggcggca cgatcctgtt gaccgcgcgg
     4801 agcaaggagt tcatgacgcg cgagggccag gagaaggccg tcgccacgct caagcgccat
     4861 ggtatcgagg gcctgatcgt catcggcggg gaaggctcgt tccacggggc gcgcgtgctc
     4921 ggcaccgaat ggggcgtgcc ggtcgtcggc gtgccggcca cgatcgataa cgacatcgcc
     4981 ggcacagact tctccatcgg cttcgataca gcggtgaacg tcgcgctgga ggcgattgat
     5041 aagatccgcg ataccgccgc gtcccatgag cggatcttcg tgatcgaggt gatggggcgc
     5101 tcggtcggct tcatcgcgct cgaggtgggc ctggccggcg gcgccgaggc gatcctcatc
     5161 cccgaggtgc cctacgacct cgataccgtc tgcaagcgga ttgccagcgg gcgcgcgcga
     5221 ggcaagatct cctccatcgt cgtcgtcgcg gagggcgc
//
LOCUS       DAOSCV010000201         5257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9912_length_5257_cov_3.050750, whole genome shotgun sequence.
ACCESSION   DAOSCV010000201 DAOSCV010000000
VERSION     DAOSCV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9912_length_5257_cov_3.050750"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="PLY56_06330"
     CDS             <1..1386
                     /locus_tag="PLY56_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21132.1"
                     /translation="LLTLARRDWGRHAPEVARAWRWFCDGYSQYPTAHIFGYYGPMHD
                     GPAWPLYLIPRRLPLAPTWQIGYPPSGDYIAECVTNRFTLREILTLCRRMAERWERGV
                     RILKKLRPHFADNPECLADIGVATALGLQFRSGYNILRFYALREELAVAAAPETRLDL
                     LSRMREIVLDEMAIDDELIPLAEADSRLGFHSEAEGYKYTPEMLRWRKRQLQRLLDEE
                     FPEVEARVHEPEPLFPEYTGERPAGAWLSCPRVEHAPAMDGLPFGGVWDELPTAECNH
                     WLRQVYNVERWKRCAYDPHDHVPVAEEDHRGRATTWKACRDARWLYLGVCCEAGDLGD
                     FSGNAVQVMVERFRTEPRRIFTVNSDGSARYTEDDGYIPREAPAFRVVSRIEGGRWSF
                     VLRLPLSWLGRGGAKGLAPLRINVERALPVPGKEGAASASWAKKVPARGRLVWGYLNP
                     ATDFGWLLFDR"
     gene            complement(1491..2387)
                     /locus_tag="PLY56_06335"
     CDS             complement(1491..2387)
                     /locus_tag="PLY56_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ21133.1"
                     /translation="MFIDCHAHAYRKRPPAYSFSTAEQVLERYDELGIEKGALLPIVS
                     PEIYLPQANEDILDMVEQYPDRFFAFCNIDPRALTNSADAPLGNLLRYYKDRGCRGLG
                     EVMPNLPVMHPMVQNLFRHAEEVGLPVTTDGSDQLTGDFGLYDDPGLPQLEHTLQRFP
                     NLIILGHGPVFWSEIARLETPGERGYIFRIGGGQVGRLPKGPVKEEGVVPKLMRLYPN
                     LYGDLSDGTAYNAIARDPEYGPKFLTEFQDRLLFGTDICYPTMAIPLVDLLIEWRDTG
                     KISEEVFRKVARENAIRLFNLE"
     gene            complement(2438..5155)
                     /locus_tag="PLY56_06340"
     CDS             complement(2438..5155)
                     /locus_tag="PLY56_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ21134.1"
                     /translation="MQRAFRTLLLTACAILWLTIQPVAAAPIEFHVAPGGNDKAAGTA
                     KAPFRTLARARDAIRALKRGKGLPEGGARVILHGGVYRLEEPFTLGPEDSGTAKAPII
                     YMAAPGERPVISGGRIIRGLRRQADGSWSVTLPEAAGHRWVFRQLFINGRRYIHARSP
                     NDGQFYIVRSVPDENDPNRKAKDRFAFRGNDIQPWPNLSDVEIRVTFSWNDGHFPIQA
                     VDTENRIVTLAGPAVWELPKPGMPTCGYIVENHPGACDAPGEWQLNRETGELRVIPFE
                     GEDLSDAEVIAPVIECLVLAKGDPEKGRYVEHVRFHGLSFQHAAWHLPPDGFSTPQAA
                     CKLGAALEFHAARHCAVTRCEVACVGRYAIWFDLDSSYNEIRRCHLHDLGAGGVRIGD
                     AERPAGYERVAHHNVVDNNFIHNGGHIHPGATGIFMAYGHNNVFSHNEVSDLRYTGIS
                     LGWTWDIVYSGTRENIVEYNHVHHVMRALEDGGGIYSLGLTPGSVIRNNLVHHVGSSI
                     NPVGHGIYIDGGSSGLLVENNICHDCGDGGIRIQHGTSCITVLNNISAFCGFGLGIDS
                     ERTNIFQYNIVLLDGAGTPFRDVPEWQSYNKIIDRNLYWRLDGKPIKFLGFTWEEWRK
                     KEGIKDIWYTPRMDAHSRIADPKFVDAKERDFRFQPDSPALQMGFRPIDTSRVRLYGE
                     KEWTSLPARVQLSPLLPQEIQPRLVLPADNFDDARPGQKPALASVVENVEKGAFVEIT
                     DRRALSKPHSLRFVVAPGLTYYYPHIYYQPAVTGDIRLRLEFDLYREPGAMLWSEWRR
                     TAAIDKVGPCFYIEKDGQLLLNEKRPAGVRLPEGEWIHFEITDGVGALADARWEMKIT
                     GQDGRVLFEGKDLPCGPDFDRLLWLGFVAHGTEPAEMYIDNISLRRVEP"
BASE COUNT          841 a         1677 c         1817 g          922 t
ORIGIN      
        1 ctcctgacgc tggcccggcg cgactggggc cggcatgccc ccgaggtggc gcgggcgtgg
       61 cggtggttct gcgacggcta ctcgcaatac cccacggcgc atatcttcgg ctactacggc
      121 ccgatgcacg atggacccgc gtggccgctc tacctgattc cacgccgcct gcccctcgct
      181 cccacatggc agatcggcta cccgccgagt ggtgactaca tcgccgaatg cgtgaccaac
      241 cgtttcacgc ttcgggagat tctcacgctc tgccggcgca tggccgagcg ctgggagcgg
      301 ggcgtccgca tcctaaagaa actgcgcccc catttcgcgg acaaccccga gtgcctggcc
      361 gatatcggcg tggccaccgc gctggggctg cagttccgca gtggctacaa catcctccgc
      421 ttctatgcct tgcgcgagga gctggcggtg gccgccgcgc ctgagacgcg cttggacctc
      481 ctctcccgga tgcgcgagat cgtcctggat gagatggcga ttgacgacga gctgatccct
      541 ctcgccgagg ccgattcgcg cctgggcttc cactccgagg ccgagggcta caagtacacg
      601 ccggagatgc tccggtggcg aaagcgccag ctccagcgtc tcctggacga ggagtttccc
      661 gaggtcgaag cccgggttca cgagcccgag ccactcttcc cggagtacac cggagagcgg
      721 cccgcgggcg catggctctc ctgcccgcgc gtggagcacg cgccggcgat ggatggcctg
      781 ccatttggcg gcgtgtggga cgagcttcct accgctgagt gcaaccactg gctgcgccag
      841 gtgtacaacg tcgagcggtg gaaacgatgc gcttacgacc cgcatgacca tgtgccggtt
      901 gcggaagaag accatcgagg ccgggccacg acgtggaagg cgtgccgcga cgcgcgctgg
      961 ctctacctcg gcgtctgctg cgaggcgggc gatctgggcg atttctccgg caacgcggtg
     1021 caggtgatgg tggagcggtt ccggacggag ccccgccgga tcttcacagt gaactcggac
     1081 ggctcggcgc gctacaccga ggacgatggc tatatcccgc gcgaggcgcc ggcgttccgg
     1141 gtggtctcgc gcattgaggg tgggcggtgg tcgtttgtcc tccgcctccc gctctcgtgg
     1201 ttggggcggg gcggcgcgaa gggcctggcg ccgcttcgga tcaacgtgga gcgcgcactc
     1261 ccggtgcctg gcaaggaggg ggcggcctcc gcctcctggg cgaagaaggt gccagcgcgc
     1321 gggcgcctgg tgtggggcta cctcaatccg gccaccgact tcggctggct cctctttgac
     1381 cggtaggccg ccgggcggct actgcccggg taaggcggtt tgcctggaga ggcggcttcg
     1441 ccggcggccg aaagccgccg gcgaagctga ggccttgaga gaaccctggg ttactccagg
     1501 ttgaagaggc ggatcgcgtt ctcgcgcgcc accttgcgga agacctcctc ggagattttg
     1561 cccgtgtctc gccactcgat caggagatcg acgagcggaa tcgccatcgt ggggtagcag
     1621 atatcggtgc cgaagaggag gcggtcctga aactccgtca ggaacttcgg gccgtactct
     1681 gggtcgcgcg cgatggcgtt gtaggccgtg ccgtcagaga ggtcgccgta gaggttcgga
     1741 tagaggcgca tcagcttggg cacgacgccc tcttccttca cggggccctt gggaagccgc
     1801 cccacctggc cgccgccaat ccggaagatg tagccccgct cgccgggcgt ctccagacgc
     1861 gcgatctcgg accagaagac tgggccgtgg ccgaggataa tcaggttggg gaagcgctgc
     1921 agcgtgtgct cgagctgggg cagccctggg tcgtcgtaga gcccgaagtc gccggtgagc
     1981 tggtccgagc cgtcggtagt cacgggcagg cccacttcct cggcgtgccg gaagaggttc
     2041 tgaaccatcg ggtgcatcac cgggaggttc ggcatcacct cgcccaggcc gcggcatccg
     2101 cgatccttgt aatagcggag caggttgccc aggggcgcat cggccgagtt ggtgagcgcg
     2161 cgcgggtcga tgttgcagaa ggcgaagaag cggtccggat actgctccac catatcgagg
     2221 atatcctcgt tggcctgggg gaggtagatc tccgggctca cgatggggag gagcgctccc
     2281 ttctcgatcc cgagctcgtc gtagcgctcc agtacctgct ccgcggtgga gaaggagtag
     2341 gctggcgggc gcttccgata ggcatgggca tgacagtcaa tgaacatcct tcttttctct
     2401 cctcgtggtg tgcgctgcgg tcattccgga cggcgctcta cggctcgacc cggcgcagcg
     2461 agatgttgtc gatgtacatt tcggcgggct cggtgccgtg ggcgacaaac ccgagccaca
     2521 gcaggcggtc aaagtcgggc ccgcagggga gatccttgcc ctcgaacagc acgcggccat
     2581 cctgaccggt gatcttcatc tcccagcggg catcggccag ggcgccgaca ccatcggtga
     2641 tctcgaagtg gatccactcg ccttcgggca ggcgcacccc cgccggccgc ttctcgttga
     2701 gcaggagctg gccgtccttc tcgatataga agcaaggccc caccttatcg atggcggcgg
     2761 tcctccgcca ctcgctccac agcatggcgc ccggctcgcg gtagagatcg aactccaggc
     2821 gcaggcggat atcccctgtc accgccggct ggtagtagat gtgcgggtag tagtaggtga
     2881 gcccgggcgc gaccacgaag cgcaggctat gaggtttgga gagcgcgcgc cggtcggtga
     2941 tctccacgaa ggcgcctttc tcgacgttct ccaccaccga cgcgagcgcc ggcttttgcc
     3001 ccggccgggc gtcgtcgaag ttgtccgcgg gcagaaccag gcgtggctgg atctcctgcg
     3061 gtaggagcgg ggagagctgg acgcgcgcgg gaagcgacgt ccactccttc tcgccgtaaa
     3121 gccgcacccg gctggtatcg atggggcgga agcccatctg gagcgcaggc gagtcgggct
     3181 ggaagcggaa atcgcgctcc ttcgcgtcga cgaacttcgg atcggcgatc cgcgagtgcg
     3241 catccatgcg cggggtgtac cagatgtctt tgatcccctc ttttttgcgc cactcctccc
     3301 acgtgaagcc gaggaacttg atcggctttc catccaggcg ccagtaaagg ttgcggtcga
     3361 tgatcttgtt gtagctctgc cactcgggaa cgtcgcggaa cggcgtgccg gcgccgtcga
     3421 ggaggacgat gttgtattgg aagatgttcg tccgctcgga atcgatcccg aggccgaagc
     3481 cgcagaacgc ggagatgttg ttgagcacgg tgatgcacga cgtgccgtgc tggatccgga
     3541 tgccgccatc gccgcagtcg tggcagatgt tgttctccac cagtagccca gaactgccgc
     3601 cgtcgatgta gatgccgtgg ccaaccgggt tgatcgacga gccaacgtgg tgaaccaggt
     3661 tgttgcggat caccgagccg ggagtgagcc cgaggctgta gatgccgccg ccgtcctcca
     3721 gcgcgcgcat cacatggtgg acgtggttat actcgacgat gttctcgcgc gtgcccgaat
     3781 agacgatatc ccacgtccag ccgagcgaga tgccggtgta gcggaggtcg gagacctcgt
     3841 tatgcgagaa gacgttgttg tgcccgtagg ccatgaagat gccggtggcg cccgggtgga
     3901 tgtgcccgcc gttgtggatg aagttgttgt cgaccacgtt gtggtgggcg acccgctcgt
     3961 atccggcggg gcgctccgcg tcgccgatgc gcacgccgcc ggcgcccagg tcgtggaggt
     4021 ggcaccggcg gatctcgttg taggaggaat cgagatcgaa ccagatcgcg tagcgcccga
     4081 cgcaggcgac ttcgcaccgg gtgacggcgc aatggcgcgc ggcgtgaaac tccagggcag
     4141 cgccgagctt gcacgcggcc tgtggcgtgg agaagccgtc cggcggcagg tgccacgcgg
     4201 cgtgctggaa gctgagcccg tggaagcgaa cgtgctccac gtaccggccc ttctcggggt
     4261 cgcccttagc aaggaccagg cactcaatga cgggcgcgat cacctccgca tcgctcaggt
     4321 cctcgccctc gaacgggatg acgcggagct cgcctgtctc gcggttcagt tgccactcgc
     4381 cgggggcgtc gcacgcgccc ggatggttct ccacgatata gccgcaggtg ggcatccccg
     4441 gcttgggaag ctcccacacc gccggcccgg ccagagtgac gatccggttt tccgtatcaa
     4501 ccgcctggat cgggaagtga ccatcgttcc agctgaaagt gacgcggatc tcaacgtcag
     4561 agaggttggg ccacggttga atatcgttcc cccggaaggc gaagcgatcc ttcgctttgc
     4621 ggttcggatc gttctcatct ggcacgctgc gcacgatgta gaactggccg tcgttgggcg
     4681 agcgggcatg gatgtagcgt cggccgttga tgaagagctg gcggaaaacc cacctgtgcc
     4741 cggcggcctc gggaagcgtg accgaccacg acccatcggc ctgcctgcgt aggccacgga
     4801 tgatgcgtcc ccccgagatc acggggcgct cgccgggcgc cgccatgtag atgattgggg
     4861 ctttcgccgt gccggaatct tccggcccga gcgtgaacgg ctcctccagc cgataaaccc
     4921 cgccgtggag gatcacgcgc gcgccaccct cggggaggcc tttgccacgc ttgagcgccc
     4981 ggatcgcatc gcgggcgcgc gcgagcgtgc ggaacggcgc tttggcggtc cctgccgcct
     5041 tgtcgttgcc gcccggcgcc acgtggaact cgatcggcgc ggcggcaacg ggctgaatgg
     5101 tcaaccagag gatcgcgcag gccgtgagca gtaatgtccg aaaagcacgc tgcatcgaac
     5161 gttccctccc gggtcaggca aacaggcgcg gcccctccat cgcgctcgcc gcccattccg
     5221 cggctgttcc tgatccacgt tcaccgaccg gcggtgc
//
LOCUS       DAOSCV010000202         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9934_length_5249_cov_3.644397, whole genome shotgun sequence.
ACCESSION   DAOSCV010000202 DAOSCV010000000
VERSION     DAOSCV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9934_length_5249_cov_3.644397"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1613)
                     /locus_tag="PLY56_06345"
     CDS             complement(138..1613)
                     /locus_tag="PLY56_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21135.1"
                     /translation="MAAPTEGPVNYEAFGAVGDGVADDLPAICKAHEYANAHNLPVRS
                     KPNATYHLGRQALTAVIATDTDWSTSRFIIDDTDVENHRKALFEVRSLLAPVELKIER
                     LRRDQAQVDVRPETDCWVQVESDAKRVYIRRGLNRNNGTAMRDCFIVRRDGTVEGAID
                     WDYDTITRIEARPIDEKPLLIKGGVFTTFANRMKQEKGYNYWARNIVITRSNTEVDGL
                     THYVVGETAVGHPYSGFLSISRCANVTLRNCFVSGHKTYSTIGAAGKPVNMGSYDLSA
                     NQVINLRLIGVRMNHILDSTRWGVIGTNYCKNILLENCVLSRMDVHQGVSGTYTIRRC
                     ELGYAGLNAIGRGTLLVEDSTLYGRAFISLRSDYGSTWEGDVVIRNCRWVPSGGQTCW
                     PHLIHASNDGTHDFGYPCFMPREIRIDGLFVDDSNHPEEYEGMYLFTDPDGAAKSDQA
                     RPFPYKLTEKVIIRGLKTASGKKPLLSPDPEMAKSTIVIEQ"
     gene            complement(1991..2896)
                     /locus_tag="PLY56_06350"
     CDS             complement(1991..2896)
                     /locus_tag="PLY56_06350"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOJ21136.1"
                     /translation="MSASLPRVRKVVIPAAGRGTRLLPATKSQPKEMLPVARKPVIQY
                     VVEEMVDAGLGQILIVSGRTKRAIDDHFDPDPVLQAELSRRNDRELMAQFAFEHLDAH
                     YFYVRQSEPRGLGHAVLLAEEFVAGEPFAVALGDSIVWNGGGKSLMRQLVQTHVETGA
                     AATIAVEEVTAQDVVNYGIVKPVDKVGHVFQISDLVEKPSVEDAPSRLAIAARYIFGP
                     QVFSALRDTAPGHGGEIQLTDAIRILLSAGCPVWCVRLEGGCRRYDIGNFDSYFKAFV
                     EISLQDPELGESLRAHLASLLKEEA"
     gene            complement(2903..4078)
                     /locus_tag="PLY56_06355"
     CDS             complement(2903..4078)
                     /locus_tag="PLY56_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21137.1"
                     /translation="MRHLAYVAPPLEIGLSMIIHTLANARVGLIGNPSDGYFGKTISL
                     TIKNFWAQVTLWESPTVQILPHPVYDPLEFDSLESLESVAVRDGYYGGQRLLFAACKR
                     FAEYCRTHGITLDRRGFTISYDTNIPRQVGLAGSSAIVTAAVKALMRFYDIGEREIPK
                     AVLPNLILSVESEELGIAAGLQDRVIQVYGGMVYMDFARDLMEGRGYGEYVPLDPELL
                     PGRLFLAYTEKPSDSGVIHSDVRFRFRQGDPEVVSAMREFARLADEAREALEMRDARR
                     LGELMNANFDLRRQTFGDAALGAQNLEMIGIARALGVPSKFPGSGGAVIGLFSTDEEG
                     RALHQAYTERGYRFVEAKPAPADEGSHREPNPEERGVPARALPVGHRGRIQITREER"
     gene            4531..5109
                     /locus_tag="PLY56_06360"
     CDS             4531..5109
                     /locus_tag="PLY56_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ21138.1"
                     /translation="MDAPDTLLIQKALAGDTAAFDELVKRHRDHMHRQLYSRCMDTEQ
                     IEDTVQETFVQAYRALGQFKGLSAFSTWLYRIAHNICLRKRQQARRTAALSLDEMTES
                     GGVSAPREVPDPGESPEQTLLSRELREAMDREVANLPDSWKKVFILRDVEDLSTAEVS
                     EALGISEAAVKARLHRARARLKQQLRPYLTVE"
BASE COUNT          920 a         1672 c         1678 g          979 t
ORIGIN      
        1 ctggtggcac gccgttgccg ccgcaagcgc ccggtggcag agtgttttcg ggacagtgcc
       61 cacgaacccg cgtggcgcct ggcagagaaa cttcttttca ttattctgat gaataatgcg
      121 ggctagcggc agggccgtca ctgctcgatc acgatcgtgc tcttcgccat ctcgggatct
      181 ggcgagagga ggggcttctt tccgctggcc gtcttgagcc cccggatgat cactttctcg
      241 gtcagcttgt aggggaaggg ccgcgcctgg tcgctcttcg ccgcaccatc aggatcggtg
      301 aagaggtaca tcccctcata ctcctcggga tgattgctgt catctacgaa cagcccatca
      361 atccggatct cccggggcat gaagcaggga tagccgaagt catgcgtgcc gtcgttggac
      421 gcatggatta ggtgcggcca gcacgtctgt ccgccggatg gcacccaacg gcagttgcgg
      481 atgaccacgt cgccctccca ggtgctgccg taatcgctgc ggagactgat gaacgccctg
      541 ccgtacaggg tggagtcctc gaccagcagg gtgccgcggc cgatggcgtt gagccccgcg
      601 tagcccagct cgcagcgtcg gatcgtgtag gtgccggaga ccccctggtg cacgtccatc
      661 ctcgagagca cgcagttctc cagaaggata ttcttgcagt agtttgtgcc gatcacgccc
      721 cagcgagtgg agtcgaggat gtgattcatc cggacgccaa tcaggcggag gttgattacc
      781 tggttggccg agagatcata ggagcccatg ttgaccggct tccccgccgc gccgatggtg
      841 gagtaggtct tgtgcccgct gacgaagcag ttgcgcaggg taacgttggc gcagcggctg
      901 atgctgagga agccggagta ggggtgcccc acggccgtct ctcccacgac ataatgggtg
      961 aggccgtcca cctccgtgtt cgaacgggtg atgacgatgt tcctggccca gtagttgtag
     1021 cccttctcct gcttcatccg gttggcgaag gtagtgaaaa ccccgccttt gatcaagagc
     1081 ggcttctcat cgatcgggcg ggcctcgatg cgggtgatcg tgtcgtaatc ccagtcgatg
     1141 gcgccctcca ccgtgccatc ccggcgcacg atgaagcagt cgcgcatcgc cgtgccgttg
     1201 ttgcggttga ggccccgccg gatgtaaacc ctcttcgcgt cgctctccac ctgcacccag
     1261 cagtcggttt ccggacggac atccacctgc gcctggtcgc gtcgcagccg ttcaatcttc
     1321 aactccacgg gcgccagcag tgagcgcacc tcgaagagcg ccttgcggtg gttctctacg
     1381 tcggtgtcat cgatgatgaa acgcgaggtg ctccaatccg tatcggtggc aatgaccgcc
     1441 gtgagcgcct ggcggccgag gtgataggtc gcgttgggct tggatcggac gggcaggttg
     1501 tgcgcattgg catattcatg cgccttacag atggcaggga gatcatcggc cacgccgtca
     1561 cccaccgcgc cgaacgcctc gtaattgacc gggccttccg ttggtgccgc catcggtgcc
     1621 gctcctccca ccgctggtgc cgcctggatc gggccggtct tccatcctgc ccatcccagg
     1681 atgagaagca gcgctcccag gctcgcttga cggacccgaa cacgtcgatg cctcttcatg
     1741 tgtatcacct gtcagacttt cccatgcctt cgttcggccc ggagaacgcc cgacccctgg
     1801 cgcacgctgg cacgcgccgt ggcgccggca acgatggacg ggctgccacg agggccccca
     1861 gccgtccagg tgtcagcctt cgcgctccgg gaacagctgg atgggacacg gcgtttaaga
     1921 gctccggacc caggtgcgct tacgcggggc agaccctggg acggattggg cacttgttcg
     1981 cgaacgggcc ctaagcctcc tccttcagga gtgacgcgag gtgagcgcgc aggctttcgc
     2041 cgagctccgg gtcttgcagg ctgatctcca cgaatgcctt gaagtagctg tcgaagttgc
     2101 cgatgtcata acgccggcac ccgccctcca gcctcacgca ccagacggga cagccggccg
     2161 agagcaggat gcggatggca tcggtaagct ggatctcgcc gccgtggccg ggtgccgtgt
     2221 cgcgcagcgc gctgaaaacc tgcggaccga agatgtagcg ggcggcgatg gctagacgac
     2281 tcggcgcatc ctcgaccgag ggcttctcga ctaggtctga gatctggaag acatggccca
     2341 ctttgtccac cggcttgacg atgccgtagt tgaccacgtc ctgagcggtg acctcttcga
     2401 cggcaatcgt tgccgccgcg ccggtctcca cgtgggtctg cacgagctgg cgcatcaggc
     2461 tcttgccgcc gccattccat acgattgaat cgccaagggc cacggcgaaa ggctcgccgg
     2521 cgacgaactc ctcggccaga agcaccgcat gccccaggcc gcgcggctcg ctctggcgta
     2581 cgtagaagta gtgcgcatcg aggtgctcga aggcgaactg cgccataagc tcgcggtcat
     2641 tcctgcgcga gagctctgcc tgtagcacgg gatcggggtc gaagtggtcg tcaattgcgc
     2701 gtttggtccg cccggagacg atcaggatct gaccgagacc ggcatccacc atttcttcca
     2761 ccacatactg gatgacgggc ttgcgcgcca ccgggagcat ctccttgggc tgcgacttag
     2821 tggccggaag caagcgtgtt ccacgacccg ccgccgggat aacgaccttt cgcacacgtg
     2881 gcagactggc agacatggac ggttatctct cctctcgggt tatctggatc cgaccgcggt
     2941 ggcccacggg aagcgctcgg gcgggcacgc cccgctcctc cggattcggc tcgcggtgcg
     3001 aaccctcatc tgccggagcc ggcttggcct ccacaaaacg atagcctctt tctgtgtagg
     3061 cctggtgcag ggcgcgcccc tcctcgtccg tgctaaacaa tccgatgaca gcaccccccg
     3121 accctgggaa tttgctgggc acccccaggg cgcgcgcgat gccgatcatt tcaaggttct
     3181 gggcgccgag tgccgcgtcg ccgaaggttt gtcggcgcag gtcgaaattg gcgttcatca
     3241 attcgcccag ccgccgcgca tcgcgcatct cgagggcctc gcgtgcctcg tcggcaaggc
     3301 gtgcgaactc ccgcatcgcg ctgacaacct cgggatcacc ctggcggaag cgaaagcgca
     3361 cgtcgctgtg gatcacgccg gagtcgctgg gcttctccgt ataggcaagg aagagccgtc
     3421 ccggcaacag ctccgggtcc aggggaacgt attccccgta gccacggccc tccatcagat
     3481 cgcgcgcgaa gtccatgtat accatgccgc catacacctg gatcacgcgg tcttggagac
     3541 cggcggcgat gcccagttcc tccgattcaa cgctgaggat gaggttgggt aggacggcct
     3601 tgggaatctc tctctccccg atgtcgtaga agcgcatcag ggccttcacc gcggccgtga
     3661 cgatagcgct cgagccggcc aggcccacct ggcgaggaat gttcgtatcg tacgagatag
     3721 tgaacccccg tcggtcgagg gtaatcccgt gcgttcggca gtactccgca aaccgcttgc
     3781 atgcggcgaa gagaagacgc tggccgccgt agtagccgtc tcggacggcg accgactcca
     3841 gcgactccag ggagtcgaac tccagcggat cgtacactgg gtgcggcagg atctgcaccg
     3901 ttgggctctc ccacaaggtc acctgagccc agaagttctt gatagtcagt gagatcgtct
     3961 tgccgaagta gccatccgat gggttgccga tcaatcctac ccgagcgttt gcgagtgtgt
     4021 ggataatcat cgatagaccg atctccaggg gcggcgccac gtacgcgagg tgtctcaatc
     4081 atgccttgga cctcaacccg tcgggcccgg tggtacgagc acgcttcctt gcctgtgttc
     4141 tgtcgggcac cggtgaactc cttcatcggc cgccggtccg gccccgcggt cggctggcag
     4201 gggggcttgg ggcagtagaa agcggcacaa cggacggcgc cttggggctc gtagagaggc
     4261 cattcatggc cggggcggtg gcgcattccg ctcgctgtag ctctcgggcg gtcggtggag
     4321 agcgacgcca tgaatgccgc cactcggaat ctcgccctcc cgagtgcgag gttgaccgcg
     4381 tggcgccgcc cggctataat gagctatcgc cacgatcaga ggaatgcgcg attgcgcgcc
     4441 gttcgcgacg gcagctcatc cgcagcggat cccgaatcgc tccgtggcga gccagcagcg
     4501 atggaaacag gtgtagtggt gaggaggagg atggacgcac ccgatacgct cttgatccag
     4561 aaagccttgg cgggagacac agcggcgttc gatgagctcg tcaagcgtca ccgcgatcac
     4621 atgcatcgcc agctctacag ccggtgcatg gatacggagc agatcgagga cacggttcag
     4681 gagacgtttg tgcaggccta tcgggcgctc ggccagttca agggtctctc cgcgttttcc
     4741 acgtggctct atcgcatcgc gcataatatc tgcctgcgca agcggcagca agcccggcgc
     4801 actgccgcgc tctctctcga tgaaatgacg gagtctgggg gcgtttctgc gcctcgcgag
     4861 gtgccggacc ccggcgagag ccccgagcag accctcctca gccgcgagct gcgggaggcg
     4921 atggatcgcg aggttgccaa cctcccggat tcctggaaga aggtgttcat cctgcgcgat
     4981 gtggaggatc tctccaccgc ggaggtgagc gaggcgttgg ggatcagcga ggcggccgtc
     5041 aaggcacgtc tccaccgcgc gcgagcacgc ctgaagcagc agctgcgccc ctacctgacc
     5101 gtcgagtagg ccccccgatc cggcgcggcg gagcaccaac caagaggaaa gggcaggttc
     5161 attgaacctg ccctcttgga gcgggagacg agactcgaac tcgcgacctt ctccttggca
     5221 aggagacgta ctaccactgt actactccc
//
LOCUS       DAOSCV010000203         5245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9941_length_5245_cov_3.451252, whole genome shotgun sequence.
ACCESSION   DAOSCV010000203 DAOSCV010000000
VERSION     DAOSCV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_5245_cov_3.451252"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLY56_06365"
     CDS             <1..382
                     /locus_tag="PLY56_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21139.1"
                     /translation="LYAGFPDQIVTGTWVDQGFPHFSGVLEYSQPVTIKADYFDYRLM
                     LAWENAAEIVDVLVNGRPVGVRPWPPNAVDISEHAFAGTNLITLRVTNTAVNALLGQP
                     RPSGILGAVRIEPYARCEVKVPKD"
     gene            419..1531
                     /locus_tag="PLY56_06370"
     CDS             419..1531
                     /locus_tag="PLY56_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOJ21140.1"
                     /translation="MDNSKSSLRICVVGAGHGGSAMAAHLALLGFPVRLYNRSEARIK
                     PILALGGIDLITRDIEDAPYGLAELEVVTTNMAEALDGVDLILVATRRNGHRFIAEQC
                     APHLRDGQVIVLNPGITCGAIEFYCVLREAGVKADVTVAEAQTFIYASRMLNPAQVRI
                     QRVKNSIPVAAIPATRTADVVKLLNRAYPQFVMGSNVINTSFDNIGSAFAPAVTVLNA
                     GRIESTHGDFEYYVEGVTPSVARILEAIDRERVAVAAGLGFKSISAREWLYVAYDATG
                     SNLYEAMKSNRGYQGIKSPLTTNHPFITEDVPMTLVPIASLGELVGVSTPTIRTIIHL
                     ASLLHEVDYWREGRTVERLGLAGCSVEQIRSLVLGE"
     gene            complement(1686..2990)
                     /locus_tag="PLY56_06375"
     CDS             complement(1686..2990)
                     /locus_tag="PLY56_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ21141.1"
                     /translation="MRRSLGWIRTCAGLAAGCAALWLSGCGGAGTPTWSSTGPAYPGS
                     SRSSASLGSVGTPATPAGIVRGMVEAPARPTGFARPIALFEWLRIGAPAYAEARLARM
                     PLADARVCLIDRATRETVSTVVTTDAKGEFRIDRVTLSPRFSVRASEGQQTLEAVVPQ
                     GGDQEVRVVVNEATTVGAEAARQAEAEGLSPEGAVLLAQQITEAQEEYQTAHPAEVPD
                     LTRPEEIQARARKHLLRTADAAVAAALKKADKAEAWHAVASAQYVAREKMKLPAKVRL
                     TPAQTEALVSAITREETCAVNTDKLLAALKKGGIKARNGKAVTKDDLTAALASLRETF
                     PSLKEVKLEKVPVLVALLMVEQKDTGFQVTSREQVKGFLKELVGEVDEKPASSKEKQQ
                     ASANGKSAEAKEKGNGGAGAAKGEKKGAAKPGKPASTGASAS"
     gene            complement(3246..3908)
                     /locus_tag="PLY56_06380"
     CDS             complement(3246..3908)
                     /locus_tag="PLY56_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ21142.1"
                     /translation="MYIIVMGGGQVGYHLTRTLMQKEHEVLLVEKDPRRAARITKELG
                     PVVFEGDGCEFRTLQQVGTNRADVVAAVTGDDEDNLVMCQISKRKFGVPRTIARVNDP
                     RNEEIFRRLGIDETVSSTKVIDDLIEQEMETGDVIPLAALRRGDLEIVEASIRQGSPA
                     ANVAIKDLPLPEESLISCIIRDGKAMIPQGDTVLHDDDVVIALVRANQAAALQAALRG
                     TA"
     gene            complement(3915..4352)
                     /locus_tag="PLY56_06385"
     CDS             complement(3915..4352)
                     /locus_tag="PLY56_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ21143.1"
                     /translation="MKVIIVGIGRVGSSLANMLDREGHQVTVVDRDALALQRRLSPDF
                     SGTKLVGNAMDQDVLERAGIRQADVLLTITEGDNRNIMIAQIAKVVFGVPKVMARIVD
                     PLRAQAYRELGIESIDQTTLVSEHMRRVVLGETASELAAQREE"
     gene            complement(4405..4704)
                     /locus_tag="PLY56_06390"
     CDS             complement(4405..4704)
                     /locus_tag="PLY56_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21144.1"
                     /translation="MRDTTRGARQRSGANRTPWSSSRGRLMFNMILSGIATVFGLILV
                     FAPRLLGANSDSRMLGGVLAGYGLVRGYYFYRRWQSHAASQPPAGNQPGARRRRG"
     gene            5039..5242
                     /locus_tag="PLY56_06395"
     CDS             5039..5242
                     /locus_tag="PLY56_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ21145.1"
                     /translation="MPTGKVKWFNASKGYGFIEMDEGRDVFVHYTAIQVPGFRTLDEG
                     QVVNFEVVDSPKGLQAAHVTLVD"
BASE COUNT          870 a         1687 c         1706 g          982 t
ORIGIN      
        1 cctctacgcg ggctttcccg accagatcgt gactggcacg tgggtagatc agggcttccc
       61 gcacttctcg ggggtgctgg agtactcgca gccggtcacc atcaaggcgg attacttcga
      121 ctaccgcttg atgcttgcct gggagaacgc cgccgagatt gttgacgtgc tggtcaatgg
      181 gaggccggtc ggggtccgcc cgtggccgcc aaacgcggtg gatatctcgg agcatgcgtt
      241 tgcgggcacg aacctgatca ccctgcgagt gaccaacacg gcggtgaatg ccctgcttgg
      301 ccagccgcgt ccctctggca tcctgggcgc ggtgcgcatt gagccctacg ccaggtgcga
      361 ggtgaaggtg cccaaggatt agaccggatg ggaggtttgg agatgctggg gtgcttccat
      421 ggataatagc aagagcagtt tgaggatctg cgtggtgggc gccgggcacg gcggttcggc
      481 gatggcggcg catctggcgc tactgggctt tccggtgcgc ctctacaacc ggagcgaggc
      541 tcggatcaag ccgatcctgg cgctcggcgg gatcgacctc atcacgcgcg acatcgagga
      601 tgcaccctat gggctcgccg agctagaggt ggtgaccacg aacatggccg aggcgctcga
      661 tggggtggat ctcatcctgg tggcgacccg gcgcaatggc caccgcttca tcgcggagca
      721 gtgcgcgccc cacctgcgcg acggtcaggt catcgtcctc aaccctggga tcacctgtgg
      781 cgccatcgag ttttactgcg ttctgcgcga ggccggcgtg aaggcggatg tgaccgtggc
      841 cgaggcacag actttcatct acgccagccg catgctcaac ccggcccagg tgcgtatcca
      901 gcgagtgaag aacagcatcc cggtcgccgc cattccggca acacgcaccg ctgatgtcgt
      961 gaagctgctc aaccgggcct atccccagtt cgtgatgggc tcgaacgtga tcaacaccag
     1021 ctttgataac atcggctcgg ccttcgcgcc cgccgtgacg gtgctcaacg ccgggcgcat
     1081 cgagagcacg cacggcgatt tcgagtacta cgtggaaggg gtgaccccct cggtggcgcg
     1141 tatcctcgaa gcgattgacc gcgagcgtgt cgccgtggcg gccggcctgg ggttcaaatc
     1201 gatctcggcg cgcgagtggc tctacgtcgc ctatgatgcg accggcagca acctctacga
     1261 ggcgatgaag agcaaccgcg gctaccaggg gatcaagtcg ccgctgacca cgaaccaccc
     1321 cttcatcact gaggatgtgc cgatgactct ggtgcccatc gcctcgctgg gcgagctggt
     1381 cggcgtgtcc acaccgacga ttcgcaccat catccacctg gcgtcgctgc tgcacgaggt
     1441 cgattactgg cgtgaggggc gcacagtgga gcgcctcggc cttgccgggt gcagcgtgga
     1501 gcagatccgg agcctggtac tgggggagtg agccctggcg ggcttagata gctgctgcga
     1561 gggtggtgac cctgccccgg gcgagcgccc ggggcacacc gcttgcacga gatggagagc
     1621 cgcgcgggag attggctgct ccattcccgt gggatcccaa ccgggatcgc tctacaggcc
     1681 gcgtcctagc tggcgctagc tcccgtggat gctggcttgc cgggcttggc cgcgcccttc
     1741 ttctctcctt tggccgcgcc ggcaccgccg ttgcccttct ccttcgcctc ggcggacttc
     1801 ccattggcag aagcctgctg cttttccttc gaggatgccg gcttctcgtc gacctcgccg
     1861 accaactcct tgaggaagcc cttcacctgc tctcgcgagg ttacctgaaa gccggtatcc
     1921 ttctgctcca ccatcaggag cgccacgagc accggcacct tttcgagctt cacctctttg
     1981 agggagggga aggtctcgcg caacgacgcg agcgccgccg tgaggtcatc tttggtgacg
     2041 gccttgccat tccgggcctt gatgcccccc ttcttcaggg cggcgaggag cttgtcggtg
     2101 ttgaccgcgc acgtctcctc ccgggtgatc gcactcacca gcgcctccgt ctgagccgga
     2161 gtgaggcgca ctttggccgg cagcttcatc ttctctcgcg cgacatattg cgcggacgcc
     2221 acggcatgcc atgcttctgc cttgtccgct ttcttcaggg cggcggcgac cgcggcatcg
     2281 gcggtgcgca ggaggtgctt tcgggcgcgc gcctggatct cctccgggcg cgtcaggtcc
     2341 ggcacttcgg caggatgggc agtctgatac tcctcctgcg cctcggtgat ctgctgtgca
     2401 aggagcaccg cgccttcggg actgaggcct tctgcctccg cctggcgcgc ggcctcggcg
     2461 cccaccgtcg tggcttcatt cacgacgacg cgcacctcct gatcgccccc ctgcggcacc
     2521 accgcttcca gggtctgctg gccttcgctg gcgcgcaccg agaagcgggg gctgagagtg
     2581 acgcggtcga tgcggaactc gcccttcgcg tctgtggtca ccacggtcga gacggtttcc
     2641 cgagtggcac ggtcgatgag gcagacgcgg gcatccgcca ggggcattcg ggcgaggcgc
     2701 gcttcggcgt aggcgggagc gccgattcgc agccactcaa agagggcgat ggggcgggcg
     2761 aagccggtcg ggcgcgcggg ggcttccacc atgccacgca cgatgccggc gggagtggcc
     2821 ggagttccca ccgatcccag gctggcgctg gaacgagagc tgcccgggta ggcaggcccg
     2881 gtgctggacc acgtcggcgt gccagcgccg ccgcatcccg agagccagag cgcggcacag
     2941 ccagccgcca ggccggcaca cgtacggatc caccccagag aacgtctcat cgcatcactc
     3001 ctccgaacca gcgcgcgtct ctccgagaga cgctgctact gggacgttcg ccacgcgggc
     3061 gaagtttccc ctcagagacg cttgtgtctg ggagcatagg cacggcctgc tgggggcccg
     3121 gactgaccac cgggggccca gatcttgaag ggctgcccga tgggcgcagc accgtcgcgg
     3181 actcctgatc ccccctcgtg ggcatcgcct gcggccggct ctgcccgatc cggcggaccg
     3241 cgcgattagg cggtgccgcg gagcgccgcc tgcagggcgg ctgcctggtt ggcacggacg
     3301 agcgcgatga cgacatcatc gtcgtgcaga acggtatcgc cctgggggat catcgccttg
     3361 ccatcgcgga tgatgcagga aatcagcgac tcctcgggaa gaggtagatc cttgatcgcc
     3421 acgttggccg cgggtgagcc ctggcggatg ctcgcctcga cgatctccag atcgccccgc
     3481 cgcagggcgg cgaggggaat gacgtcaccg gtctccatct cttgctcgat cagatcgtcg
     3541 atcactttgg tgctcgatac ggtctcgtcg atccccaggc gtcggaagat ctcctcgttg
     3601 cgcgggtcgt tcacacgcgc gatcgttcgg ggaactccga acttgcgctt cgagatctgg
     3661 cacatgacga ggttgtcctc gtcatccccg gtcaccgcgg cgacgacatc ggctcgattg
     3721 gtgcccacct gttgcagcgt gcggaactcg cacccatcgc cctcgaagac gaccggaccg
     3781 agttcctttg tgatccgcgc cgctcgccgc ggatccttct ctacgaggag cacctcgtgt
     3841 tctttctgca tgagggtgcg tgtcagatgg tatccaacct ggccgccacc catgacgatg
     3901 atatacatcg ggccctactc ttcccgctga gccgcgagct cactcgcggt ctcccccagc
     3961 accacgcgcc tcatatgctc gctcacaagc gtcgtctgat cgattgactc gataccaagc
     4021 tcgcggtatg cctgagcgcg caggggatcc acgatgcgtg ccattacctt gggaacgcca
     4081 aagacaacct tggcaatctg ggcgatcatg atgtttcggt tatcgccctc ggtgatcgtg
     4141 aggagaacat ccgcctgtcg gatgccggca cgttccaaaa cgtcctggtc catggcattg
     4201 ccgacgagct tggtgcccga gaaatcgggg ctgagacggc gctgcagggc gagcgcgtcg
     4261 cggtctacca ccgtcacctg atggccctcg cggtcgagca tgttggccag gctggaacca
     4321 acgcggccga tccccacaat gatgaccttc atggtcactc ccccataacg atgcttctcc
     4381 gggatagatg cgccgcggag aagtctaacc gcgccggcga cgagctccag gctggtttcc
     4441 tgcgggcggt tggcttgcag catgggattg ccagcgccgg tagaagtagt agccgcggac
     4501 aaggccgtaa ccggcgagca ccccgccgag catcctggaa tcactgttgg ctccaagcag
     4561 gcgcggggcg aacaccagga tcagcccgaa cacggtggcg atcccagaaa ggatcatatt
     4621 gaacatgagg cgaccgcgcg acgacgacca gggagtccgg tttgctccac tgcgctggcg
     4681 ggcccctctc gtagtatctc tcacggcatc acgtttccgt tctctaactc ccatcggtcg
     4741 cacgccagta tacaaccatt gccagggagt gtcaaggaat cgggagagcg cgcacgggtg
     4801 gggagagacc gcggggaggg gcatgctacg cgggcgggct gcacgagagc ttagctacca
     4861 tgcggattct ttcgcagcgc ctgctttaca catcacacag gagtgtgcta tactagccgt
     4921 gcagttaacc tgcaccggtg cttccgatga tgcagagcga agctgttcag aggtccgtcg
     4981 atccgcctcc ccgtgctttc atctgccgcc acggagggat ctcaaggagg tagtaggaat
     5041 gcctaccggt aaggtgaagt ggttcaacgc atccaagggc tacggattca tcgagatgga
     5101 tgaggggcgc gatgtcttcg tgcattatac cgccattcag gttcccggct tccggaccct
     5161 ggatgagggt caggtggtga atttcgaggt tgtcgatagc cccaagggtt tacaagcggc
     5221 gcatgtgacc cttgttgact agggc
//
LOCUS       DAOSCV010000204         5241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9948_length_5241_cov_3.453914, whole genome shotgun sequence.
ACCESSION   DAOSCV010000204 DAOSCV010000000
VERSION     DAOSCV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9948_length_5241_cov_3.453914"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLY56_06400"
     CDS             <1..363
                     /locus_tag="PLY56_06400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ21146.1"
                     /translation="VPTPPNPGIALALRAQSFWDWAFLILVVVVMAPIFEEIFFRGAL
                     YGAIRRHAGAGAAIAVTSLFFALVHPQLPLGFLPILVLGIVFAIVVELRRSLIPSIVA
                     HMLNNGVALLLLAVVRTP"
     gene            360..662
                     /locus_tag="PLY56_06405"
     CDS             360..662
                     /locus_tag="PLY56_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOJ21147.1"
                     /translation="MKRQPSKAPSALPATTLRVRVQPRASRSEIVGWRDGVLQVRLTA
                     PPVEGAANAACIELLADTLGVPKSAISLASGAHAREKLFRIVGLSDEEARRLLPAP"
     gene            690..1235
                     /locus_tag="PLY56_06410"
     CDS             690..1235
                     /locus_tag="PLY56_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ21148.1"
                     /translation="MIRTLLTGISGTGKSTVIRELAARGHKAVDLDSDEWSKWVPFVP
                     FPGVKEVPGDPEQEWVWREDRIQALLSTEDAEMLFVSGCASNMRQFLDQFDHIILLSA
                     PADLIVERLITRTNNPYGKDPEERARVLGHLESVEPLLRRRAGHEINTQLPLGQVVEE
                     VLRIARSRPREARSCAHRAAG"
     gene            complement(1232..2368)
                     /locus_tag="PLY56_06415"
     CDS             complement(1232..2368)
                     /locus_tag="PLY56_06415"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016442084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOJ21149.1"
                     /translation="MGGRAVEQYRRQCIGILTSGGDCPALNAAIRGIGKAALGHYGME
                     VIGILDGFRGLVENRARPLAASDLSGILTKGGTFLGTSRDKPHRIPVADGTEVDLSAV
                     AIANYRRLHLDCLVCLGGGGTLKNAHRLMKQGGINVVHLPKTIDNDVWGTDACFGYDT
                     ALQIVSEAIDRLHSTADSHHRIMVIETMGHNAGWLALGAGVAGGADVILIPEIPYNLD
                     YVAESLVARLRKGRRFSIVVVAEGAAPISPDAESGNGGKKRKKRNSGELSVAGAGFTG
                     IGAELAAELQARTHLEARVTVLGHLQRGGTPSPADRILATRLGTAAADLIARGQYGVM
                     VAVRGGECVPVPIEEVAGKLRTVPLDHPLIDTARKVGTCLGDQL"
     gene            complement(2543..2893)
                     /locus_tag="PLY56_06420"
     CDS             complement(2543..2893)
                     /locus_tag="PLY56_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21150.1"
                     /translation="MADVSGVEEQVEETAQAQPAAEGQGEAKPHPGALREAEVVAEME
                     IGRTSKLVFQVGKRRTGDMAFDIREYVDRPARGNSPGYTGPTRSGFRLHEENYEEFLE
                     AVKSIGRALGYHVD"
     gene            complement(2984..3994)
                     /locus_tag="PLY56_06425"
     CDS             complement(2984..3994)
                     /locus_tag="PLY56_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ21151.1"
                     /translation="MTLRLTRTATPVQTPAPPVQPPLQPTGTVDPVVSIVKRIAPAVV
                     SIQTLTPGEQTFFGVTPPREGQGSGVIVTPDGRILTNSHVVAGSAEIRVRLSNGETLP
                     GRLIGQDRRSDIAVVQVQGRNLPTAPLGDSSALQVGEAVVAIGNPMGFQSTVTTGVVS
                     ALNRTLEPQPGTVLEDMIQTDAAINPGNSGGPLVNSRGEVVGINTLIISTAQGLGFAI
                     PINGAQRVAEQLVERGRVVRPWLGVATVPLPEEMAAAVGLPVGTGAYVAEVFPGSPAA
                     RAGIERGDILVRAGGQLLRGTDALREVIQSRQVGAQITVELLRDGERIEIPVRLGEAP
                     VP"
     gene            4251..5129
                     /gene="hisG"
                     /locus_tag="PLY56_06430"
     CDS             4251..5129
                     /gene="hisG"
                     /locus_tag="PLY56_06430"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ21152.1"
                     /translation="MDKRVLKIGLPKGSLQESTFGLFRKAGYNLSVNGRSYHVDMDDP
                     ELSGLLIRPQEIPRYIEDGIIDCGLTGYDWILSSGADLVQVGELVYAKVGFGPVRIVC
                     AVHKDDPIQDVRELQGKRIATEYVELTKMWLATHEVDAQVEFSWGACEMKVPDLADAI
                     VANTETGNSLRANGLRIAFELMKSTTRFVANHRSWEDPWKRKKAENLAMLLQGAINAQ
                     DLVGLKMNVRRRDLDAVINVLPALRKPTISTLSDPEWVAMETILDPRKVRDLVPELKR
                     AGAEGIAAYPLTMVVY"
BASE COUNT          867 a         1785 c         1627 g          962 t
ORIGIN      
        1 gtccccacgc cgccgaaccc gggaatcgcg ctcgctctga gggcacaaag cttctgggat
       61 tgggcgttcc tcattctggt ggtcgttgtg atggcaccca tcttcgagga gatcttcttc
      121 cgcggggcac tttatggggc gatccgcagg cacgccgggg cgggcgctgc catcgccgtc
      181 acgagcctct tcttcgccct cgtccacccg caactcccgc tcgggtttct cccaatcctg
      241 gtcctgggga tcgttttcgc gatcgtcgtc gagctgcgcc gttcgctcat cccgagcatc
      301 gtggcgcaca tgctgaacaa cggcgtggct ctgttgctcc tggcggtggt aaggacacca
      361 tgaagagaca accatccaaa gcgccctcgg cgctgccggc cacgacactt cgggtgcgcg
      421 tgcagccacg cgcatcgcgc tcggagattg tcggctggcg cgacggcgtg ctccaggtgc
      481 gcctcacggc cccaccggtc gaaggcgccg ccaacgccgc gtgcatcgag ctcctggccg
      541 acacgctggg tgttcccaaa tctgctattt cccttgcttc cggggcccac gcccgcgaga
      601 agctctttcg cattgtcggg ctgagtgacg aggaggcgag gcggctgctg cccgcgccgt
      661 agacgcgccc actctgacga gaggatccca tgattcgcac gcttctgacg ggcatctctg
      721 gaaccgggaa gtcaaccgtc atccgcgagc tggcggcacg cggccataag gccgtggatc
      781 tggatagcga cgagtggtcc aagtgggttc cgttcgttcc gtttcccggc gtgaaggagg
      841 tccctggcga tccggaacag gaatgggtgt ggcgggagga tcggattcag gcgcttctct
      901 ccacggagga cgccgagatg ctgttcgtga gcgggtgcgc gagcaacatg cgccagttcc
      961 tcgatcagtt cgaccacatc atcctgttga gcgcgcccgc cgatctcatc gtggagcggt
     1021 tgatcacccg aaccaacaac ccgtacggca aggatccgga agagcgggcg cgcgtgctgg
     1081 gccatttgga gagcgtggag ccgcttctcc gaaggagagc cgggcacgag atcaacaccc
     1141 aactccccct gggtcaggtg gtggaagagg tgctccgaat cgcgcgctcg cggccacgcg
     1201 aggctcgctc ctgcgcgcat cgcgccgctg gctagagctg gtcgcccagg caggtgccca
     1261 ccttgcgcgc cgtgtcgatc aggggatggt cgaggggcac cgttcgcagt ttgccggcga
     1321 cctcctcgat gggtacggga acgcactcgc cgccgcgcac ggcgaccatc accccatatt
     1381 gcccccgggc gatcagatcc gcggcggccg tccccaggcg ggtggccagg atgcggtcgg
     1441 ctgggctggg cgttccgccg cgctgcaagt ggccaaggac ggtgacgcgc gcctccagat
     1501 gggtgcgggc ctgaagctcc gcggccagct cggccccaat cccggtgaac ccggcgcctg
     1561 caacggatag ctctcctgag ttgcgtttct tgcgtttctt tccgccgtta ccggattccg
     1621 cgtccggcga tatcggcgcg gcgccttccg ccaccaccac gatgctgaag cgtcggccct
     1681 tccgcaaacg cgccaccagg ctttcggcga cgtaatccag gttatagggg atctccggga
     1741 tgaggatgac gtccgcgccg ccggccacgc ccgcaccgag cgcgagccac cccgcgttat
     1801 gccccatcgt ctcgatcacc atgatccggt ggtggctatc cgccgtggag tgcagccggt
     1861 caatcgcctc cgacacgatc tggagggccg tatcgtaacc gaagcaggca tcggtgcccc
     1921 agacatcgtt gtcgatcgtc tttggcaggt ggacgacgtt gatcccgccc tgcttcatca
     1981 ggcggtgggc gttcttcagg gtcccgcccc cgcccaggca gacgaggcaa tcgaggtgaa
     2041 gccggcggta gttggcaatc gccacagcgg agaggtccac ctcggtgcca tcggcgacgg
     2101 ggatgcggtg cggcttgtcg cggctggtgc ccagaaaggt gccccccttg gtcaagatgc
     2161 cagagaggtc gctcgcggcg agggggcgcg cccggttttc caccaggcca cggaagccat
     2221 cgaggatgcc gatcacctcc atgccgtaat gcccgagcgc cgccttgccg atgccacgaa
     2281 tggccgcgtt cagcgccggg cagtcgccgc cagaggtgag gatgccgatg cattgtctcc
     2341 ggtactgctc caccgctctc cctcccatac cgcttgcctg gcagctttgg gcaagccgcg
     2401 catgtaccca cgagggatct attcaacgcg ccgcggtggg ctccctgcct gagacgcgct
     2461 ctcgccggcg cgatggcgag ctccgggccg gcctcactcg catccctggc cccccgggca
     2521 tcctccggag gaccctccgg gctcaatcca cgtgatagcc cagcgcgcgc ccgatagatt
     2581 tcaccgcttc caggaactcc tcgtagttct cctcgtgcag tcggaagcca ctgcgcgtgg
     2641 ggcccgtgta gcccgggctg ttgccgcgcg ccgggcggtc cacatactcc cgaatatcaa
     2701 acgccatgtc gcccgtgcgt cgcttgccta cctggaaaac gagcttgctc gttcgcccga
     2761 tctccatctc cgcaacgact tcggcttctc gcaacgcgcc tggatgcggc ttggcctctc
     2821 cctgcccctc ggccgccggc tgtgcctgcg ccgtctcctc aacctgctcc tcaactccac
     2881 tcacatccgc catctatctc tctccttgcg cacgccccgc tgaggggacc atcgggctcc
     2941 gattccgttc ttccccatcg gtgccatcat ttcgcttact aaatcagggt actggtgctt
     3001 ctcccaggcg gaccggtatt tcgatcctct cgccgtcgcg taggagttcc acggtgattt
     3061 gggcccccac ctgccggctc tggatcactt ctcgcagtgc atcggtaccg cgaagcagtt
     3121 gaccgcccgc gcgcaccagg atatcgccac gctcaattcc cgcccgagcc gccgggctgc
     3181 cggggaaaac ctccgccaca taggcccccg tgccaacagg caggcccacc gcggccgcca
     3241 tctcctccgg gaggggaacc gtggccactc caagccacgg ccggacgaca cgcccgcgct
     3301 cgacaagctg ctcggccacg cgctgcgcgc cgttgatcgg aatggcgaag ccaagcccct
     3361 gcgccgtgga gatgatcagc gtgttgatcc cgaccacctc gccacgggag ttcaccagcg
     3421 gccccccaga gttgcccggg ttgatcgccg catccgtctg gatcatatcc tccagcacgg
     3481 tccccggctg gggctccagc gtccggttca acgcgctcac cacgcccgtg gtcaccgtgc
     3541 tctggaagcc catcgggttg ccaatcgcca ccaccgcctc gccaacctgg agcgcggatg
     3601 agtcgcccag gggcgcagtc ggcaagttgc gaccctgcac ctgcactact gcgatgtcgc
     3661 tgcgccgatc ctggccgata agcctgccag gcagcgtctc tccattggag aggcgcacgc
     3721 gaatctcggc cgagccggcc accacgtggc tattcgtcag aattcggcca tcgggggtga
     3781 caatcactcc tgatccctgc ccttcccgcg gtggcgtcac tccgaagaag gtctgctccc
     3841 cgggggtgag cgtttggatc gagacgaccg ccggcgcaat ccgcttgacg atgctcacca
     3901 ccggatcgac cgtgcccgtg ggctgcagag gcggctgcac tggcggcgcc ggcgtctgaa
     3961 ccggtgtagc cgttcgagtc agcctaagcg tcactccagt tcccacggct gcacccagca
     4021 gcgcggccaa gaacaatgcg actaggctcc tgtgctgcat ctaagcctca ctccctaaca
     4081 cgcctggcag gcaggcgcct tctgcagtat accacgactg cacgcggctg aaaaccccca
     4141 gtggaagccc cctttgttcc tgtatcaggg aggagacgtg ccattgcacc gcgaaagcag
     4201 agggtgttgg gtggttctgc ccttactttt gtgaacgagg actaactgct gtggacaaac
     4261 gcgtgctcaa gattgggctt ccaaaaggga gcctgcagga atcgactttt ggccttttcc
     4321 gtaaagccgg atacaacctg tcagtgaacg gtcgctccta ccatgtcgac atggacgacc
     4381 ctgagctgtc cggtcttctc atccgcccgc aggagattcc gcgctacatt gaggatggca
     4441 tcattgactg cggccttacc ggatacgact ggatcctctc ttctggcgcc gatctggtgc
     4501 aggttgggga gttggtctac gccaaagtgg gcttcggccc tgtgcgcatt gtctgcgccg
     4561 tgcacaagga tgatccgatc caggatgtgc gcgagcttca gggcaagcgc atcgccacgg
     4621 agtacgtcga gctcacgaag atgtggctgg ccactcatga ggtggacgct caggtggagt
     4681 tctcatgggg cgcctgcgag atgaaggtgc ccgatctcgc cgatgccatc gtcgccaaca
     4741 ccgagacggg gaactcgctc cgggccaacg gccttcgcat cgccttcgaa ttgatgaaga
     4801 gcacgacgcg ctttgttgcc aaccaccgct cctgggagga tccctggaag cggaagaagg
     4861 cagaaaacct ggcgatgctg ctgcagggcg ccatcaatgc ccaggatctc gtcggattga
     4921 agatgaatgt tcgccggcgc gacctggatg cggtgatcaa cgttctgcct gccttgcgga
     4981 agccgacgat ctcgacgctt tccgaccccg agtgggtcgc catggagacg atcctcgacc
     5041 cgcggaaagt tcgggatctg gtgcccgagc tgaagcgcgc gggcgcagag ggaattgctg
     5101 cttatcccct caccatggtc gtctactgat ctcgcggacc ctgctgggat tccgagataa
     5161 ggaatccgaa gccacggagg cattgagcaa cccggcgcgc ggcgataagt caccgcgctg
     5221 cttgcgagcg aagccctgcc g
//
LOCUS       DAOSCV010000205         5237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9959_length_5237_cov_3.734080, whole genome shotgun sequence.
ACCESSION   DAOSCV010000205 DAOSCV010000000
VERSION     DAOSCV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9959_length_5237_cov_3.734080"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLY56_06435"
     CDS             complement(<1..611)
                     /locus_tag="PLY56_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOJ21153.1"
                     /translation="MFGQDTIAAIATAPGEAAIGVIRISGPSAIAVAAAIFHPASGAD
                     VARLASHRVYLGEIRVPDTGEPVDQVLLSLFRAPRSYTGEDVVEISGHGGPVPLRRIL
                     SLVLAQGARLAHPGEFTSRAFLNGKLDLAQAEAVMDVVAARTDRSLGVAIRQLEGRLS
                     QVVRSARDALLPLVAHIEATIDFPEDVPEMPRADLRAQIEAAH"
     gene            complement(627..1214)
                     /locus_tag="PLY56_06440"
     CDS             complement(627..1214)
                     /locus_tag="PLY56_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ21154.1"
                     /translation="MRAIDDTDKAVEEVTEPTEGAESEPAQATGEAPEEGQRAATEFG
                     AHVRDVTQRIVDLMGLQLEARVAQADRHQVEIDLQGPDARIIIGKHGQTLEALQQIVS
                     LIVNKTATRRHRVLVDAEGYQQRRRARREQELLEMVRLDAQRAKETGQEAVIQYAVGP
                     YERRIVHMAFANDPDFITYSEGEEPYRYVVISPRD"
     gene            complement(1231..2460)
                     /locus_tag="PLY56_06445"
     CDS             complement(1231..2460)
                     /locus_tag="PLY56_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOJ21155.1"
                     /translation="MSFVRQWRIWGVLLLLALALFWPGRAPQAAEIPVPVRTRLEQVD
                     QMRREGQSEEAARILTDYGKEVRQDKVAHAAVQFVLADIKRQDLKDEKGAFALYNQLA
                     MTQIEGEVEIPGFGTVDIQDSALQVQDQRNQSHWLYKMMAGIVYALAHFPVIGKALEP
                     HTGVLAILLLSIVIKVALTPLTKKSFRAMREMQKLQPLMKDLQQRYRDKPQELQKRTM
                     ELYQEHGVNPLSGCLPMLLQMPIIILLWRAIALYQYPLSQESFLWIKSLAVADVPLAL
                     IYAASLYASSKLTMMPAADPAQEQQQKMMAIMMPVMFFFLFKSYPAGFILGWFFFNVL
                     TTAQQWYLMRSCTDEAAPGAGPGSSDGGNGGGGNGGGPRRPVSPNGGASRGKSKPTAS
                     RTINKRKKHVAAYHRGR"
     gene            complement(2574..2780)
                     /gene="yidD"
                     /locus_tag="PLY56_06450"
     CDS             complement(2574..2780)
                     /gene="yidD"
                     /locus_tag="PLY56_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007305055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ21156.1"
                     /translation="MKALALQLIRGYQAVSRLMPPTCRYYPSCSEYTAQAIAKYGVLH
                     GSYLGLKRILRCHPFHPGGYDPVQ"
     gene            complement(2789..3148)
                     /gene="rnpA"
                     /locus_tag="PLY56_06455"
     CDS             complement(2789..3148)
                     /gene="rnpA"
                     /locus_tag="PLY56_06455"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ21157.1"
                     /translation="MLPRRERLTRSGAFRAVVARGRSFRGRFLALYFLPRSSGPTRLG
                     VAAGKKVGGAVQRNRAKRLLREAFRGLKEKLDGAYYVVLVARPAAARAGLDAVRTELE
                     SLMWRARIVARPAGESS"
     gene            complement(3263..3397)
                     /gene="rpmH"
                     /locus_tag="PLY56_06460"
     CDS             complement(3263..3397)
                     /gene="rpmH"
                     /locus_tag="PLY56_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ21158.1"
                     /translation="MKRTYQPHNRRRQRTHGFRARMRTPGGRRVLAARRMRGRHRLAV
                     "
     gene            3585..>5237
                     /gene="mutL"
                     /locus_tag="PLY56_06465"
     CDS             3585..>5237
                     /gene="mutL"
                     /locus_tag="PLY56_06465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ21159.1"
                     /translation="MGRIAILDEATIGQIAAGEVIERPVSVVKELLENSIDAGARRIT
                     ISVEEGGCRAITVVDDGEGMERDDAVLAFQRHATSKIRDACDLATTRTLGFRGEALTS
                     IAAVSVLEMATRARGVVSGVRLAARGGTITDLQEAGAPEGTSITVRHLFFNAPVRRRY
                     LKSAATELGHIADCVAQYAVAHPGISFQLSQSGETILATTGQGHLFDAIVAILGAEAG
                     RQLIPVDRPGEQCSLSGYIARPTLTRGTRSGQWFFVNARPIRSRVIAHAVEQAYHTLV
                     PSGRHPIAVLLLTMDPALVDVNVHPAKSEVRFLSEGEVHRFVSHALREALGSLDLTPR
                     VEAHPGSPLPVAGTATLPDTLASGHAGSDAPALPFAEGDTCGKAPPEVRAQIHDTYIL
                     AEGEDGLLIIDQHTAHERALFEQILSGTAGDLMRPLPLAVPFVLSLDSRQASALRDSL
                     DALEAVGFSLHPFGRDAYLVRAVPAALASTDCESLLRDAIEEILSGSAPRSIEARREQ
                     IASFLACRSAVKSGDRLTLQEMQAIVRGLYALQSRFTCQHGRP"
BASE COUNT          889 a         1847 c         1589 g          912 t
ORIGIN      
        1 tcgtgggcgg cctcgatctg cgcccggaga tcggcgcgcg gcatctcggg aacatcctcc
       61 gggaaatcga tcgtggcttc gatgtgtgcg acgaggggga gaagggcatc acgcgcgctg
      121 cgaacgacct gcgacagcct gccctcaagc tggcggatcg ccaccccaag agagcggtcg
      181 gtacgcgcgg cgaccacatc catcaccgct tcggcctgtg ccaagtcaag cttgccattg
      241 aggaaggcac gactggtgaa ctcccctggg tgagccaggc gggctccctg ggccagtacg
      301 agggagagga tgcggcgcag tggcaccggg ccgccgtgcc cggagatctc cacaacatcc
      361 tcgccggtgt aggagcgagg cgcgcgaaag agggagagaa gcacctgatc gaccggctca
      421 cccgtatcgg gcacgcgaat ctcgcccaga tagacacgat gagaggcgag acgagcgacg
      481 tcggcgccgc tcgccggatg gaagatcgcc gcggcaaccg cgatcgcgga cggcccgctg
      541 atccggatca cgccgatggc ggcttccccg ggcgcggtgg cgattgccgc gatggtgtct
      601 tggccaaaca tgggccgaac cggtgctcag tcgcgaggtg agatcaccac gtagcggtac
      661 ggctcctcgc cctcgctata ggtgatgaag tcaggatcgt tggcaaaggc catgtggacg
      721 atgcggcgct cgtaggggcc caccgcatac tggatcacgg cctcttgccc tgtctccttg
      781 gcgcgctgcg catcgaggcg aaccatttcg agcagttcct gctcgcgacg ggcgcgacgg
      841 cgctgctggt atccctcggc atccaccagc acccgatgcc gccgcgtcgc cgtcttgttc
      901 acgatcaggc tgactatctg ctggagcgcc tccagcgtct gtccatgctt gccgatgata
      961 atgcgcgcgt ccggcccttg cagatcgatc tccacttggt gtcggtcagc ctgggccacc
     1021 cgggcctcca actggaggcc catcagatcc acgatgcgct gcgtgacgtc gcgcacgtgc
     1081 gcgccaaact ccgttgccgc gcgctgaccc tcttccggcg cctcgccggt cgcctgagcc
     1141 ggctcgctct ccgcgccctc ggtgggttcc gtcacttctt ccaccgcctt gtcggtgtcg
     1201 tcgatcgctc tcatgcctct tgcgtcctgc ctatctgccg cgatggtacg ctgccacgtg
     1261 cttcttccgc ttgttgatgg tccgcgacgc cgttggcttc gacttgccgc gagacgcgcc
     1321 gccattcggt gagacgggac ggcgtggccc gccaccgttg ccgccaccac cattgccacc
     1381 gtccgacgag cccggaccgg cgcccggcgc ggcctcatcg gtgcagctcc gcatcaggta
     1441 ccactgctgc gcggttgtca gcacgttgaa gaagaaccag cccaggatga agcctgccgg
     1501 ataggacttg aacaggaaga agaacatgac cggcatcatg atggccatca tcttctgctg
     1561 ctgttcttgc gccggatccg ccgccggcat catcgtcagc ttggacgaag cgtagaggct
     1621 cgcggcgtag atgagcgcca gtggaacatc cgccaccgcc aggctcttga tccacaggaa
     1681 cgactcctga gacagcggat actgatagag cgcgatcgcc cgccacagca ggatgatgat
     1741 cggcatctgc agcagcatcg gcaagcagcc cgagagcgga ttgacgccgt gctcctgata
     1801 cagctccatc gtccgcttct gcagctcctg cggcttatcg cgataccgct gctgcagatc
     1861 cttcatcagg ggctgcagct tctgcatctc gcgcatcgcg cggaacgact tcttcgtcag
     1921 gggcgtcaac gccaccttga tcacgatgct gagcagtagg atcgccagca cgcccgtgtg
     1981 gggctccagc gccttcccga tgaccgggaa atgcgccagg gcgtagacga tgccggccat
     2041 catcttatag agccagtggc tctggttcct ctgatcctga acctgcagcg ccgaatcctg
     2101 gatatccacc gtgccaaagc cggggatctc cacctccccc tcaatctgcg tcatggcgag
     2161 ctgattgtag agggcaaagg cacccttctc gtccttcaga tcctggcgct tgatatccgc
     2221 cagcacaaac tggaccgcgg catgcgcgac cttatcctgg cgcacctctt tgccgtagtc
     2281 cgtgaggatt ctcgctgcct cctcagattg tccttcgcgg cgcatctggt ctacctgctc
     2341 cagacgcgtg cgcaccggca ccgggatctc cgctgcctgg ggcgcgcgcc ctggccagaa
     2401 aagcgccagc gccagaagga gcaacactcc ccatatccgc cactgtctca caaagctcat
     2461 attgttagcc caaggtaatc tcaatagtcc agaaaagccc aatcattccg gcgcactcgc
     2521 cgcagccccg cccggatcct ggaatccggt ggcttccgct tgcggctcgc gacttactgc
     2581 accgggtcgt aaccgccggg gtggaacgga tgacagcgca ggatccgttt caggccaagg
     2641 tagctcccat ggaggactcc gtacttggca atcgcctggg cggtgtattc ggagcagctc
     2701 gggtaatagc gacaggtggg cggcatcagc cgcgaaaccg cctggtatcc ccgaatcaac
     2761 tggagcgcca atgctttcat ttgctccttc atgaactctc ccccgcaggt cgggccacga
     2821 tccgagcgcg ccacatcagc gactccaact ccgttcgaac cgcgtccaga ccggcccgcg
     2881 ccgctgccgg ccgggccacc aggaccacat agtaagcgcc gtccaacttc tccttcaatc
     2941 ctcgaaacgc ctctcgcagg aggcgcttgg cccgattccg ctgcaccgcg ccgccgactt
     3001 ttttgccagc ggccacccca agacgggtgg ggccgctgct ccgaggcaga aagtagagcg
     3061 caagaaacct tccccggaag cttcgacccc gggcaaccac cgcccggaac gctccggatc
     3121 tggtcaaccg ctctcgcctc ggcagcatgc agcccctcaa taacactcaa tgcgaagcat
     3181 gggaaaacgg gacgaccggc acgcgcaccc gcggtgccgg ctccgcttct cctttttcca
     3241 cgctccaaag gttgagcccc actcacacgg ccagtctgtg ccggcctcgc atgcgccgcg
     3301 ccgcgagaac acggcgcccg ccgggggtgc gcatccgcgc gcgaaatcca tgggttcgtt
     3361 gccgccgccg gttatgcggc tggtaagtac gcttcactga acgctcctcc agtcctgtcc
     3421 cgggccgcgc catcggcgag agccccgact caacaagaac actgcggctt attatagcac
     3481 aaaagatggg gcccgggcaa ctgtgggtca gcggctgcct gccaggccca tgcttgacgc
     3541 gtcgcgccct gcctgatata ctcctgccga gaggggggat cgccgtgggc cgcatcgcga
     3601 tcctggatga ggcgactatc ggccagatcg ccgctggcga ggtcatcgag cgccccgtct
     3661 ccgtggtgaa ggagctcctg gagaacagca tcgatgccgg cgcccgccgc atcaccatct
     3721 ccgtggaaga agggggatgc cgcgcgatca ccgtggtgga tgatggcgag ggcatggagc
     3781 gcgacgacgc ggtgctcgcc ttccagcgcc acgccaccag caagatccgc gacgcttgcg
     3841 atctcgccac gacacgcacg ctcggcttcc gaggcgaggc tctgacaagc atcgccgctg
     3901 tcagcgtcct cgagatggcg acgcgggccc gaggcgtggt ttccggggtg cgcctcgccg
     3961 cgcgcggggg caccatcacg gatctgcaag aagctggcgc ccccgagggc acgagcatca
     4021 ccgttcgcca cctctttttc aacgcgcccg tgcgccgtcg ctacctgaaa agcgcggcca
     4081 ccgagctggg ccacatcgcc gattgcgtgg cccaatacgc cgtcgcgcac ccaggcatca
     4141 gctttcagct ctcccaatcg ggcgagacca tccttgccac caccggccag ggccacctct
     4201 tcgatgcgat cgtggccatc ctcggcgctg aggcggggcg gcagctgatc cccgtcgacc
     4261 gacccggcga gcagtgctcc ctctctggct acatcgctcg cccaacgctc acccgtggca
     4321 cgcgctccgg ccagtggttt ttcgtgaatg cccgtcctat ccgcagcagg gtgatcgcgc
     4381 acgcggtgga gcaggcctac cacacgctcg ttccgtccgg gcgtcaccct atcgccgtgc
     4441 ttctcctcac gatggacccg gccctcgtcg atgtgaacgt tcatccagct aagagcgaag
     4501 tgcgctttct ctcggaagga gaggtgcacc gcttcgtctc gcatgccctc cgggaggcgc
     4561 ttggaagcct ggacctgact ccccgagtgg aagcgcatcc cggctcgcct ctcccagtgg
     4621 caggaactgc cacgctcccg gataccctgg cgtccgggca cgctggttct gatgcgccag
     4681 cgctcccttt tgccgaaggc gacacctgcg gcaaggcgcc cccggaggtg cgggcgcaaa
     4741 tccacgatac ctatatcctc gccgaagggg aagacggcct tctaatcatc gaccagcaca
     4801 ccgcgcacga gcgcgcgctt ttcgagcaga tcctctctgg caccgcgggc gacctgatgc
     4861 gccccctgcc cctcgccgtt cccttcgtcc tcagcctcga ctctcgccag gcatccgccc
     4921 tccgcgatag tctcgacgcg ctggaagcgg tcgggttctc gctccatccg ttcggtcgcg
     4981 atgcctacct ggtgcgcgcc gtgcccgccg ccctggccag caccgactgc gaatcgctcc
     5041 tgcgcgatgc catcgaggag atcctgagcg ggagcgcccc gcgcagcatc gaggcgcggc
     5101 gggagcagat cgcctcattc ctcgcgtgcc gcagcgcggt gaagtccggc gaccggctga
     5161 cgctgcagga gatgcaggcg attgtccgcg gtctctacgc cttgcagtcg cgtttcacct
     5221 gccagcacgg ccggccc
//
LOCUS       DAOSCV010000206         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9971_length_5232_cov_3.159552, whole genome shotgun sequence.
ACCESSION   DAOSCV010000206 DAOSCV010000000
VERSION     DAOSCV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9971_length_5232_cov_3.159552"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLY56_06470"
     CDS             complement(<1..1008)
                     /locus_tag="PLY56_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOJ21160.1"
                     /translation="MKKRTLALMLLLGIAAAWTALRAQATVSLVEPAVTPAAGQPGEY
                     TFSVKYVHPDDLPPITPGVQLHIETTTGSPVPGSPFTLSTNDSSPVYSTGVVFSTTVS
                     LPAGSYKHRFTASDGGPTVTLGPLDGPLVNTPPTLSGGSVTPASGSSTTLFEYRVTYT
                     DADGHAPDFVRAIIDGGQELLLTRADSNPYETGSLFTGKTTLAPLFHSFHFEASDGYV
                     ATPVVLKDTGNVPFAGPVNGAKFVGKVTNTATGDPVSGATVTTGQTQTVTDSQGDYTL
                     FGPVGSYELTAYPPFGENLLEAQVSVSVAAGEEQTRNFALPVGGVLEGVVRDPDSAPV
                     EG"
     gene            complement(1005..2072)
                     /locus_tag="PLY56_06475"
     CDS             complement(1005..2072)
                     /locus_tag="PLY56_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21161.1"
                     /translation="MRRAHYAVIVGVAALACAAYVLGGAGMQGERAGSKWWAPVGADT
                     RGTTKSAGRADDAPDALLLRLVGEWYGIPEPALAQAARDRLSPAEVALAANIAARVGR
                     PFAEILECRKAGQEWDAIGRRFGLEPPMLAEPIRPPGSQEDEALFRELLSRNFQLSEL
                     EVARLRREGFAMPDIVVAAVLAGGDAAAVSETLHARKQGESWQRAGARFVGRRLMAEE
                     ASARAAARRGAAERNAGGADTAESTAYARALLTGYYGVRPRDVDAALNRGVPAMELLI
                     AANAAARSGQEFSQVLQLRGQGLSWREIERRLGLAMGDLCKLPDPGARRTGRPGEGGD
                     ASGAKKAVGTGREPGGRGKSR"
     gene            complement(2115..>5232)
                     /locus_tag="PLY56_06480"
     CDS             complement(2115..>5232)
                     /locus_tag="PLY56_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21162.1"
                     /translation="AVRGSSAGADVSPTASLMWCDQGLVYVDAAGEQILLHPGYETLV
                     AKGEAPPPPTPMSDNTHALNQILNLEASLSQSESTVVLEGASVGSGSPEEQRMIDAER
                     VVDDLFKAYSEEQSAKVEALIHDNFITNDVPGLARNRMDVILACDADFEQLANTHFVL
                     QPATSRRFYTDNDHVSLYKRWVIDAILTSGARFQQGGETLFVIAADMRIIRWEGDLPF
                     GRFKPEPGADDEMLIATPTVLTPPAGDAPDVIVTQADAALGSTGVELTNVTAQIPING
                     VMVQTSASQGSTIQVGNTSTIVATNATFTVNGAQVTADNVSIQNSSTFNATGVQVNVD
                     GTQMQAGALNVSGGTVQLQDVILTDAQGNMVSLVTATINPGPDGGPPVISGVTDTGQT
                     IHTGGGQIVYEPAPGAPLLAGRGERLGDGDGFTFATSAVTAGPSDLYVSGGRLDLDGL
                     AADLGVADFAGVVQAPATGYTLDLIPLDSLVPGQVLVVQLAGGGYAKIRVRAGTTAEQ
                     LYFDWVYNPDGAALSRGRRWPSRAAITFDAKVYNRTASGHQRFIPGGVESVAEFRAET
                     YRNLSNGASVEAYIAPRLSTDRQLDSKTAIVDRFGVRYFMPNQFEVTLGDISSEFTPY
                     TLNRTILGLSAWRNFSLGPVGTARLSTAIGPRWQAGSAFRLDGFVTGIRLSTEELRDL
                     GPYIEDMVVGLNLVKSYRGKRQRGDYLHSTVASMDASLRLKNGLSLMAELARSRATDN
                     KRDYNGDAVLLRAAYRVGPLRLILDRERVGTGFTTVSGSAAADQERTNFWLRYRPNEW
                     LSANVNFLHTRDNLTGNKAFTTRLDMPRYGLSISPFYPILSDGFLRNLTVDLLLRETE
                     RDSDDEQHTVNKKARTLSVSLIQRMGDFFLTLGRDEIRDLDYNSSGVSRRGHATDLSL
                     RWRPRGGFLNIPISPVLGYRTGRENYTGGTAGPVSSRITSRRIGVDLGDEDWLAVELG
                     YELLDNSRTDLGGYDRRILNVDLTRALDREGNRLARISYRFSDNDQENGARSYSEGQF
                     TGSISQKF"
BASE COUNT          880 a         1777 c         1698 g          877 t
ORIGIN      
        1 cccctcgaca ggagccgaat cgggatcgcg cacgacgccc tcgagcactc caccaacggg
       61 cagcgcgaaa ttacgcgtct gctcctcgcc cgcggcaacg ctcaccgaga cctgggcctc
      121 gagcaggttc tccccaaacg gcgggtaagc cgtgagctcg taactgccca ctggcccgaa
      181 aagcgtatag tccccttgcg agtccgtaac cgtttgagtc tgcccggtgg tgaccgtggc
      241 cccgcttacc gggtcgccgg tggcggtgtt cgtcaccttc ccgacaaact tcgcgccatt
      301 cacagggccg gcaaacggca cgttgcccgt gtccttgagg accaccggcg tggccacgta
      361 tccgtcgctc gcctcgaagt ggaacgaatg gaacagcggc gccagcgtag tcttgccggt
      421 gaagagagag ccggtctcat agggattgga atccgcccgc gtgagcaaga gttcctggcc
      481 gccatcgatg atggcgcgca cgaagtccgg cgcgtggcca tcggcgtcgg tgtaggtgac
      541 ccggtactcg aagagggtgg tgctggagcc gcttgccggc gtgacgctcc ccccggagag
      601 ggtggggggg gtgttgacaa gggggccatc aagcgggcca agggtgaccg tcggcccccc
      661 atcggacgca gtgaagcggt gcttgtagga gccggcggga agagagaccg tcgtgctgaa
      721 gacgactccc gtactataga cgggactgga atcattggta gacagggtga agggcgatcc
      781 cgggacgggc gatccggtcg tagtctcgat gtggagctgc acacccggcg tgataggggg
      841 aaggtcgtcc gggtgaacat acttcaccga aaaggtgtac tctcccggct ggccggcggc
      901 aggggtcacc gctggctcca ccaagctcac cgtcgcctgc gcccgcagcg cggtccaggc
      961 tgcggcgatg ccgaggagta gcatgagagc gagcgttctc tttttcatct cgatttcccc
     1021 ctcccgccgg gctcacgccc cgtcccgacg gcctttttgg ccccagaggc atctccgccc
     1081 tcgccggggc ggcccgtccg gcgcgctccg ggatccggga gcttgcacag gtcacccatg
     1141 gccagtccca ggcgccgctc aatctcgcgc cagctcaatc cctgtcctct cagctggagc
     1201 acttgcgaga actcctgtcc agagcgcgcg gccgcgttgg cggcgatcag caactccatc
     1261 gcgggcaccc cgcggttcag cgcggcgtcc acatcccgcg ggcgaacgcc gtaataccct
     1321 gtcaggagcg cacgggcata cgcagtggat tccgccgtat ccgcgccgcc cgcgttcctc
     1381 tccgccgccc cacgacgcgc agccgcgcgt gcggaagcct cctcagccat caggcgccgg
     1441 cccacgaagc gcgcccccgc acgctgccag ctctcgccct gcttgcgcgc gtggagcgtc
     1501 tcggagacgg cggcagcatc cccaccggcg aggaccgcgg cgaccacgat atcgggcatg
     1561 gcaaaaccct cgcggcgcag ccgggcaact tccagctcgg agagctggaa gttgcgcgat
     1621 aggagctcgc gaaagagcgc ctcatcctct tgtgaaccgg gcggccgaat cggctccgcc
     1681 agcatcggag gctccaggcc gaatcgccgg ccgatagcat cccactcctg cccggccttc
     1741 cggcactcca ggatctcggc gaaaggacgc cccaccctcg ccgcgatgtt cgccgccagc
     1801 gcgacctcgg cgggagagag cctgtcgcgc gccgcctggg cgagcgccgg ctcaggaatg
     1861 ccgtaccact cccccaccag gcgcaggagc aaggcgtccg gcgcgtcatc cgcgcgtccg
     1921 gcggacttcg tggtgccccg cgtgtcggcg cccacaggcg cccaccactt cgagcccgcg
     1981 cgctcgcctt gcatcccagc cccgcccagc acgtaggcgg cgcaggccag ggcggccact
     2041 ccaacgatca cagcgtagtg cgcacgacgc atgagcgtcc tctctcactt ccaggatgcc
     2101 agcgctcccg gttgtcagaa cttctggctg atgctccccg tgaactgccc ttcgctgtag
     2161 gagcgcgcgc cattctcctg gtcgttatcc gagaagcgat aggagatccg cgccaggcgg
     2221 ttcccctcgc gatccagcgc ccgggtcagg tccacgttca ggatccgccg gtcgtaaccg
     2281 ccgagatcag tgcggctgtt atccagaagc tcgtagccca actccacggc cagccagtcc
     2341 tcatcgccca gatccacccc gatgcgacgc gacgtgatcc ggctggagac cggcccggca
     2401 gtgcctccgg tgtaattctc acgcccggtg cggtaaccaa ggaccggcga gatggggatg
     2461 ttcaggaagc cgcccctcgg gcgccaccgc aggctaaggt ccgtcgcatg gccacgccgg
     2521 ctcaccccgc tcgagttgta atcgaggtcg cggatttcat cgcgtcccag ggtgaggaag
     2581 aaatccccca tccgctggat gagcgagacc gagagcgtcc gcgccttctt gttgacggtg
     2641 tgctgctcgt catcgctatc gcgctccgtc tctcgcagca ggagatccac ggtgaggttt
     2701 ctcaggaagc catcactgag tattgggtag aaggggctga tgctgaggcc gtagcgcggc
     2761 atatccagcc gggtggtgaa cgccttgttg ccagtgaggt tgtcacgcgt gtgcaggaag
     2821 ttcacattgg cgctcagcca ctcattgggc cggtagcgca gccagaaatt cgtccgctcc
     2881 tggtcggcag cagccgagcc gctcaccgtc gtgaacccgg tgcccacgcg ctcgcggtcg
     2941 aggatgaggc gcagggggcc cacgcgatac gccgcgcgga ggagcacggc atcgccatta
     3001 tagtcgcgct tgttgtcggt cgctcgcgaa cgggccagct ccgccatcag gctcaggccg
     3061 ttcttcaggc gcagcgaggc atccatactc gccaccgtgc tgtgcaggta gtctccccgc
     3121 tggcgcttgc cgcgatagga cttgaccagg ttgaggccca ccaccatatc ctcgatgtag
     3181 ggccccaggt cgcgcagctc ctccgtagag agccggattc cggtgacgaa gccatccagg
     3241 cggaaggcgg agcccgcctg ccagcgcggc ccgatggcgg tggagaggcg cgcggtacca
     3301 accggtccca gggagaagtt gcgccaggcc gagaggccca ggattgtccg gttgagcgta
     3361 tagggggtga actcgctgga gatgtctccc agggtgacct caaactggtt aggcatgaag
     3421 tagcgcacgc caaaccggtc cacgatagcg gtcttgctat ccaactgccg atccgtgctc
     3481 aggcgtgggg cgatataggc ttcgaccgaa gcgccattgc tcaggttgcg gtatgtctcc
     3541 gcgcggaact ccgccacgct ctccacgccg ccggggataa agcgctggtg cccggacgcg
     3601 gtgcggttat acaccttcgc gtcgaaggtg atcgccgccc ggctgggcca gcgccggcca
     3661 cgcgaaagcg ccgcgccatc cgggttgtaa acccaatcga agtaaagctg ctctgccgtg
     3721 gtgcccgcgc gcacccggat cttggcatag ccgccgccgg cgagctgaac gaccagcacc
     3781 tgcccgggca ccagggagtc gagcgggatc agatccaggg tgtagccggt ggcaggcgcc
     3841 tgtacgacac cagcaaaatc ggcaaccccc aggtcggccg cgaggccatc caggtcgagc
     3901 cgaccgcccg acacatagag gtcgctgggg ccggccgtga cggcactggt tgcgaaggtg
     3961 aagccatccc cgtctccaag gcgctcccct ctccctgcga gcagcggcgc gcccggagcc
     4021 ggttcataga cgatctggcc cccacccgta tggatcgtct gcccggtatc cgtcacgccg
     4081 gagatgacgg gcggcccccc atcagggccc gggttgatcg tcgccgtgac cagcgagacc
     4141 atgttccctt gcgcgtccgt aagaatgaca tcctgaagct ggacggtgcc cccggagacg
     4201 ttgagcgctc cggcctgcat ctgggttccg tccacattga cctggacccc ggtcgcgtta
     4261 aacgtgctcg agttctggat ggagacgtta tcggccgtca cctgcgcgcc gttcacggtg
     4321 aacgtggcgt tggtggcgac aatcgtgctc gtgttcccca cctgtatcgt gctcccctgg
     4381 ctcgcgctcg tctgaaccat gacgccgttg atcgggatct gcgccgtcac gttcgtcaac
     4441 tccacgccgg tgctgcccag cgccgcatcc gcctgggtca cgatcacgtc cggcgcgtcg
     4501 cctgccggtg gggtgagtac cgtgggcgtc gcgatcagca tctcatcatc ggcgcctggt
     4561 tccggcttga agcgcccgaa ggggaggtcg ccctcccagc gtatgatgcg catatcggca
     4621 gcgatcacga agagcgtctc gccgccctgc tggaagcggg caccactggt gaggatggca
     4681 tcgatcaccc agcgtttgta cagggagacg tggtcattgt ccgtatagaa gcggcgcgaa
     4741 gtggccggct ggagaacgaa atgggtattc gccaattgct cgaagtcggc gtcgcaggca
     4801 agtatcacat ccatgcggtt tcgcgccagg cccggcacgt cgttcgtgat aaagttgtca
     4861 tggatgagtg cttctacctt cgcggattgc tcctcgctat acgccttgaa caagtcatca
     4921 accacccgct cggcatcgat catgcgctgc tcttcgggcg agccggatcc gaccgaggcg
     4981 ccttccagca ccacggtcga ttcggactgg ctgagcgacg cctccaggtt caggatctgg
     5041 ttgagggcat gcgtgttgtc gctcatgggc gtcggcggtg ggggcgcctc gcccttcgca
     5101 acgagggtct catagccggg atggagcagg atctgctcgc cggccgcatc cacatagacc
     5161 aggccttgat cgcaccacat cagggaagcg gtcgggctca catcggcgcc ggccgacgac
     5221 ccgcgcacgg cc
//
LOCUS       DAOSCV010000207         5229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9980_length_5229_cov_3.317356, whole genome shotgun sequence.
ACCESSION   DAOSCV010000207 DAOSCV010000000
VERSION     DAOSCV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9980_length_5229_cov_3.317356"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..802)
                     /locus_tag="PLY56_06485"
     CDS             complement(179..802)
                     /locus_tag="PLY56_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ21163.1"
                     /translation="MLDHLFERTVAEWAFLGLALMIAIPIHEFAHAWAARWAGDDTAA
                     SMGRVTLDPFKHLDPLGTILILLGGFGWGKPVPVNPYNFRSPRWGSLFVSAAGPASNI
                     LLALLIGVVGFRTGLLQLGADGFAGVMQQLIWLNLALALFNLIPVPPLDGSKVLLGLL
                     PSQTAITVARVFDKFGLLMLVLLLITGMVGAIIRGPAIFLYRAILGV"
     gene            complement(812..3472)
                     /locus_tag="PLY56_06490"
     CDS             complement(812..3472)
                     /locus_tag="PLY56_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ21164.1"
                     /translation="MDVITTHVGADFDALASVIAASRLYPHALPVLSGGADRNAAEFL
                     SLHQELLDLRSLKEVDLDAVTTLVVVETQSSSRLGDLGKVALRPNVRVIVYDHHQDSG
                     DLNPERAVVKRYGATTTILVEEIRARDLPVTPFEATLFLLGIYEDTGGLTFPSTTPQD
                     ADAVAWLLRQGANLEVVSGFLIRGLTPDQRALMNDLLAHVRYHDINGARVAVTSAEVP
                     HYVDEVALLAHRLADTEGADALFVLVRMQENLLVVARARSWGIDVARALEPLGGGGHE
                     RAASATIKGGDPVAVEAALLESLQQVVRPRARARDLMSWPVRSVAPDTPVSEVNRALV
                     RYGIGGMLVVENGQPVGVIDRRDIDKAIRHRLGHAPVLAFMSRDVHPVSPDTSLRDIE
                     RLMIEERVGRVPVMHEGRIVGIITRTDVIRSLHQSLPVVHRGGRVAGGQESLSPAVVA
                     DLLARRLPGRVHDILLRVGETGDELGVHVFAVGGFVRDLLIGVENLDVDLVVEGDGID
                     FARALADRLDADAALHHRFGTAVLTLPDGFRVDVASTRTELYEQPAALPVVEHSSLRD
                     DLARRDFTFNAMAIQLNAYARGRLVDPFRGRRDLRAGRVRVLHSLSFIEDPTRLFRGV
                     RFEARYGFRFAPGTERLARTAIEHHVVARLSPARLRKEVVLTLQEQRALLAIERWDEL
                     GLFPALWPGLELTRETRERLQRADPTIAWYNDLGCRARIERWLVLFAILLAPLGVESA
                     TRFMETRLHLRARHREIVRAALFALPRLCAVLEQPWHAPGQMGVRASEVYWACRDLPV
                     EALLLAVIVQGSEQARDRVALYLRELRHVRPRLNGDHLIALGYPRGPLVGTVLREILD
                     RRLDGELPSVEAETEYAQERLKELVAPATG"
     gene            complement(3487..4803)
                     /gene="lysA"
                     /locus_tag="PLY56_06495"
     CDS             complement(3487..4803)
                     /gene="lysA"
                     /locus_tag="PLY56_06495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOJ21165.1"
                     /translation="MLLGTQRINAQGHLEIGGCDTVALAREFGTPLYVMDEALLRANC
                     RAYKRAFADRYPENDIIYAGKAFLCTAMCALLAEEEMCLDVASAGELHTALMAGFPPE
                     RIFMHGNNKSRAELEMALDARVHRIIVDNELELEMLIALAKERDRQVDVLFRITPGID
                     PHTHARIRTGQVDTKFGFEVPNGQAARVIERAIAHPNLNVRGIHFHVGSQLLDLEAHR
                     QAIEIAVELLAQFREDIGYVAEELDIGGGLGIRYLSSHEPPSIASYADVVVGTLDRCL
                     KEHNLPRPRLLQEPGRSIVGEAGTTLYTIGPVKEIPGVRTYVVVDGGLSDNPRPALYD
                     AVYEVLVANKANQPADLTVTVAGKHCETDTLFQDVRVARPEPGDILAVQSTGAYNYAM
                     ASNYNRFTHPMVVLVHNGQADVIVERQTLEDLVRHDVMPARLARPK"
BASE COUNT          999 a         1745 c         1691 g          794 t
ORIGIN      
        1 gcctaccgcg tgcgcaccac gctgatcacc ggtgatgggc aacgggtgga cgcaggcacg
       61 cactcgttcc agcggatccc tagcccctac tgaggggctt cgcggaagac cgccggatat
      121 gtagcgaggc gccccgtctc ttcgagacgg ggcgcctcgc tacgttactg gagcggcgtc
      181 acacccccag gatcgcgcgg tagaggaaga tcgcagggcc ccggatgatg gctccgacca
      241 tgccggtgat caacagcagg accagcatca gcaatccgaa cttgtcgaac accctggcca
      301 ccgtgattgc cgtctggctg ggcagcaagc cgaggagtac cttcgagcca tcgaggggtg
      361 gcacggggat cagattgaag agggcaagcg ccagattcag ccagatcagc tgctgcatca
      421 cccccgcgaa gccatcggcg cccagttgga gcaagccggt gcgaaagccg acgacgccga
      481 tcaacagtgc cagcaggatg ttggacgccg gtccggcggc agagacgaac agcgagcccc
      541 agcgcggact gcgaaagttg taggggttca ccggaaccgg ctttccccac ccaaacccac
      601 cgaggaggat cagaatagtg ccaaggggat ccaggtgctt gaacggatcg agggtcaccc
      661 ggcccatgga agcggctgta tcgtcgccgg cccaacgagc cgcccaggca tgggcgaact
      721 cgtgaatggg gatggcgatc atcagcgcga ggcccagaaa ggcccattcc gccaccgttc
      781 gctcaaagag atgatctagc acacttgctc cttatccagt tgccggggcc acaagctcct
      841 tcaaccgctc ctgtgcatac tccgtctccg cctccaccga ggggagttcc ccatcgagac
      901 ggcggtccag gatctcgcgc agcaccgtgc cgacgagggg gccgcgcggg tatccgagcg
      961 cgatcaggtg atcgccattc aggcgcgggc gcacgtgccg caactcgcgc aaatagagtg
     1021 ccacgcggtc gcgtgcctgc tcgcttccct ggacgatcac cgcaaggagc aacgcttcca
     1081 cgggaaggtc ccggcaggcc caatacacct cgctggcgcg cacgcccatc tgacccgggg
     1141 catgccacgg ctgctccaac acggcgcaga gccggggcaa tgcgaagagc gccgcgcgca
     1201 cgatctcccg gtgacgggcc cgcaggtgca ggcgagtctc catgaagcgc gttgccgatt
     1261 ccacccctaa cggcgccagc aggatggcga acaacacgag ccacctctcg atgcgggctc
     1321 ggcaccccaa atcattatac catgcgatcg tcggatccgc ccgttggagg cgctcgcgcg
     1381 tttcgcgcgt caactccaat ccaggccaaa gcgcggggaa tagtccgagt tcatcccacc
     1441 gctcaatggc cagcaaggcg cgctgctcct ggagggttag caccacttcc ttgcgcaacc
     1501 gcgccggcga gagccgggca acaacgtggt gctcgatggc agtgcgggcg agccgctccg
     1561 tgcccggggc gaagcgaaag ccgtagcgag cctcgaagcg cacgccgcgg aagaggcgcg
     1621 tggggtcttc gataaagctc aaggagtgca ggacgcgcac ccggccggcg cgcaggtcgc
     1681 gccggccgcg aaagggatcc accaggcggc cgcgggcgta ggcgttgagc tggatcgcca
     1741 tcgcgttgaa tgtgaagtca cgtcgggcca ggtcatcccg cagcgacgag tgctcgacaa
     1801 caggaagcgc cgcgggttgt tcgtacagct ccgtgcgcgt gctcgccaca tcgacgcgga
     1861 atccatcggg gagcgtgagc accgccgtgc caaaccgatg gtggagcgcg gcatcggcat
     1921 ccaggcggtc ggccagggcg cgggcaaaat cgatcccatc gccctccacc accagatcca
     1981 catccaggtt ctccaccccg atcaggaggt ctcgcacgaa cccacccacg gcaaagacgt
     2041 ggacgcccag ctcatcccct gtctcgccca cgcgaaggag gatatcgtgg acacgtcctg
     2101 gcaagcgcct ggccaggagg tccgccacga ccgccggcga tagactctcc tgccccccgg
     2161 ccacgcgtcc gccgcgatgc accaccggga gagactggtg cagcgagcgg atcacatcgg
     2221 tgcgcgtgat gatccccacg atccgcccct cgtgcatcac gggcacgcgc ccgacgcgct
     2281 cctcaatcat caggcgctcg atatcgcgca acgaggtgtc cggcgacacg ggatgtacat
     2341 cgcgcgacat aaaggcgagc accggggcgt gccccaggcg gtggcgaatc gccttatcga
     2401 tgtcgcggcg gtcgatgacg cccaccggct ggccattctc cactaccagc atcccaccga
     2461 tgccatagcg cacgagcgcc cggttcacct cgctaacggg cgtatccggc gcgacgctgc
     2521 gcaccggcca cgacatgagg tcgcgtgccc ttgcccgtgg ccggaccacc tgctgtaggc
     2581 tctccaaaag cgcggcctcg accgcaacag gatcgccccc cttgatggtg gcggatgcgg
     2641 cacgctcgtg ccctccgccg cccaacggtt cgagcgcgcg cgccacatcg attccccagg
     2701 agcgagcgcg cgccaccacc agcaggtttt cctgcatgcg cacgaggaca aagagggcat
     2761 cggcaccctc ggtatccgcg aggcggtgcg ccagcagcgc cacttcatcg acgtagtgtg
     2821 ggacctcggc cgaagtgacc gccacgcggg cgccgttgat gtcgtggtag cggacgtgcg
     2881 ctaggaggtc gttcatcaac gcgcgctggt cgggcgtcag gccacgaatg aggaagccgg
     2941 aaacgacttc caggttggcg ccctggcgga ggagccaggc gacagcgtcg gcgtcctgcg
     3001 gggtggtgct gggaaaggtg agcccccccg tatcctcgta gatgcccagg aggaagagcg
     3061 tcgcttcaaa gggggtaacc ggcaagtcgc gcgcgcgaat ctcctcgacc agaattgtgg
     3121 tggtcgcccc atagcgcttg accacagcgc gctccgggtt gagatcgcca ctatcctggt
     3181 gatgatcata gacgatcacg cgcacattag ggcggagggc gaccttgccc aggtcgccca
     3241 gccgtgaaga ggattgcgtc tcgaccacca cgagcgtggt caccgcatcc aggtctactt
     3301 ctttcaggct gcgcaggtcg agcagctcct ggtgcaggct gaggaactcc gcggcgttgc
     3361 gatcggcgcc gccggagagc acgggaaggg cgtgcggata gagccggctg gccgcgatca
     3421 ccgacgcgag ggcgtcgaag tccgcgccca catgcgtggt gatcacgtcc attgagccgg
     3481 cactccctac ttcgggcgcg ctaaccgtgc gggcatcaca tcatgccgca ccagatcctc
     3541 cagtgtctgg cgctcgacga tcacgtcggc ctggccgttg tgcaccagca ccaccatcgg
     3601 atgcgtgaag cgattgtagt tgctcgccat cgcgtagttg taggcgccgg ttgactgaac
     3661 ggcgagaatg tcgccaggct ccgggcgcgc cacccgcaca tcctggaaga gcgtgtccgt
     3721 ctcgcaatgc ttgccggcaa cggtgacggt gagatccgcc ggctgattgg ccttgttggc
     3781 gacgagcacc tcgtagaccg cgtcgtagag cgcggggcgc gggttgtcgg agaggccgcc
     3841 atccaccacc acataggtgc gcacgccagg gatctccttc accgggccga tggtgtagag
     3901 ggtcgttcca gcctcgccga caatcgagcg ccctggctcc tggagcaggc gcggccgcgg
     3961 caggttgtgc tccttcaggc agcgatccag cgtgccaacc accacatccg cgtaactcgc
     4021 gatcgagggg ggttcgtggc tggagaggta gcgaatcccc aggccaccgc cgatatccag
     4081 ctcctcggcg acgtagccaa tatcctcgcg gaactgggcc agaagctcga cagcgatctc
     4141 aatcgcctgc cggtgcgctt ccaggtcgag aagctgcgag ccgacgtgga aatggatacc
     4201 gcgcacgttc aggttcgggt gggcaatggc gcgctcgatg acgcgggcgg cctggccgtt
     4261 cggaacctca aagccaaact tggtatccac ctggcctgtg cggatgcgag cgtgggtgtg
     4321 cggatcgata cccggagtga tacggaacag cacatccacc tgccggtcgc gctctttcgc
     4381 gagcgcgatg agcatctcca gctccagctc attgtcgaca atgatgcggt gaacgcgcgc
     4441 gtcgagggcc atctccaact ccgcgcgcga cttgttgttg ccgtgcatga agatgcgctc
     4501 cggcgggaaa ccggccatca gggcagtgtg tagctcccct gccgaagcca catcgaggca
     4561 catctcctcc tcggcgagga gtgcgcacat cgccgtgcag agaaacgcct tcccggcgta
     4621 gataatatcg ttttctggat accggtcagc aaaagcgcgc ttgtaggccc ggcagttcgc
     4681 gcgcagcagc gcctcatcca tcacatagag cggagtgcca aactcacgtg ccagcgccac
     4741 ggtatcgcag ccaccgatct caaggtgacc ttgtgcgttg atgcgttgtg tgcccagtaa
     4801 cacccttatc cgactccttc tcgtcgtagc taccgccctc atccagccgc tccgtgccca
     4861 aaccagagag aaggctggga tccatgatgg gagagcacga tcaatatgat ggaccagatg
     4921 acaagccaag cggctcgccg ccctcaagag cagatcaaac ccatacggct cgcgcagggc
     4981 cgacgcggcg caccgcggca cggaacgccc gtagtctaac ataagccaag gggcagagtc
     5041 aaggcagggg tgccgcacgc gccggcgccg gaggtggacg gggcgccctg gctcgcgcgc
     5101 ggagccggca gctccgcgcg atgcctccgg ctcatgggat tccgacataa ggaatccgaa
     5161 gcctcggagg cagcgcgcaa cccggcgcgc ggcgataaat caccgcgctg cttgcgagcg
     5221 aagccctgc
//
LOCUS       DAOSCV010000208         5227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_9983_length_5227_cov_3.520108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000208 DAOSCV010000000
VERSION     DAOSCV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9983_length_5227_cov_3.520108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLY56_06500"
     CDS             <1..208
                     /locus_tag="PLY56_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21166.1"
                     /translation="ESPATPEEAVEPEPEPEAEPEEPEETERPPEILPTISPTEERLR
                     RLAAEDPEFVARLLRSWLINQGKR"
     gene            322..1344
                     /gene="fliG"
                     /locus_tag="PLY56_06505"
     CDS             322..1344
                     /gene="fliG"
                     /locus_tag="PLY56_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOJ21167.1"
                     /translation="MEERPVPVAQLPAVRKASIVLISLGQELSANVLKHLNRDEIEML
                     TLEIARIKHVSPDLRDAVLAEFSQMIVAQECIIEGGIEYAQGVLQQALGKETASEIIS
                     RLVTMLQVQPFDSVKSVDPTQLLQLLQNEHPQTIALVLAHLPPETSAMVLSGLPQKLQ
                     AQVAIRIVGMDRASPEVVREVEGILQARITATGTQDFDKAGGPSALADILNRADRSTE
                     RAILDDLMENSPEIAEEVKKRLFVFEDIITLGRRDIQLVLREVDGKDLALALKGAPEN
                     VRNVIYENMSERAAQSLREEMEFMGAVRLRDVEAAQQKIVSVIRALEEAGEVVISRGE
                     ENETII"
     gene            1328..2170
                     /locus_tag="PLY56_06510"
     CDS             1328..2170
                     /locus_tag="PLY56_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOJ21168.1"
                     /translation="MRPLSRVLKAPYRDHAPHVISAPVFPSFDTELLVPDEDEAAEPE
                     LLCEEEAPIEEPPEPEPEPPPEPTAEVDEEEEEEEPAGPSPEEEAAAIRAEAEQMLAE
                     ARSQVAEFLREAQQQAQAMEAEARERGFREGMEAAQDAQRRLLADLEALRAAVQAERD
                     LFFENLEPEVVRLALAIAEKIIRQEVDLHPEIVVGVARAALLQLKDAQTARVSAHPSS
                     VDALRQHLASSLPGGPSEISIHATPLVSPGGCMIDSDRGSVDARLETQMERIATALQE
                     GDTG"
     gene            2308..3606
                     /gene="fliI"
                     /locus_tag="PLY56_06515"
     CDS             2308..3606
                     /gene="fliI"
                     /locus_tag="PLY56_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOJ21169.1"
                     /translation="MSRLGEVASFLLSGRVVRVVGTVIESIGPPCAIGEVCRIHTADG
                     KEIYAEVVGFKDERLILMPMDEVGNLEPGSTVVTTGAPLRVPVGESCLGRIFDGLGRP
                     IDNGGPLLGTTLRAVHASPPDPLTRERVNQPLVTGIRAIDGLLTCGRGQRIGIFAGSG
                     VGKSTLLGCIARHSTADVNVIGLIGERGREVREFLERDLREEGLRRSVVVVATSDQSP
                     LQRLRGAAVATTFAEYFRDQGLDVVLMMDSLTRFAWAQREIGLAAGEPPATRGYTPSV
                     FGALPRLLERAGTASRGSITGFYSVLVDADDMNDPIADTVRGILDGHIVLSRDLARQG
                     HYPAIDVLSSVSRIMSEVTEEDHRQAATRMRDLLAVYREAEDLINIGAYVAGSNPRID
                     AAIAHIDAIRQFLRQDAQAISPYEETLRLLASCVGQNGAE"
     gene            3608..4051
                     /locus_tag="PLY56_06520"
     CDS             3608..4051
                     /locus_tag="PLY56_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21170.1"
                     /translation="MKPFVFNLQTVLEVRRIREEQARADLAALRYREKQEEERIARLC
                     QQRQDVHRRLVDGNAPLSGSQVLAAASTERSLDLAIHDSQRQKEEITRQREVKLEQTR
                     MVITQRKTVELLREKAWGRHLYEAAVAAERQVDELCSRRVEERAG"
     gene            4069..4719
                     /locus_tag="PLY56_06525"
     CDS             4069..4719
                     /locus_tag="PLY56_06525"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ21171.1"
                     /translation="MLENLQRVEARIAVLEQRLSAISAPPAEAGASPASSFAQALAAA
                     SRSGESGTLPPAPVAPMAAAPLRDAPANLPPPPRWVGHAPDADDAEEAVRRAARRHGV
                     DPSLAVAVARAESGFNPLAVSSSGALGLMQLMPGTARSLGVSDPFDVEQNADGGVRYL
                     AEQLRRFGDVRLALAAYNAGPNRVARYGEVPPIPETQRYVERVLEYQQQYRASGTP"
     gene            4756..5139
                     /locus_tag="PLY56_06530"
     CDS             4756..5139
                     /locus_tag="PLY56_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOJ21172.1"
                     /translation="MTDRRETMRIDGTRSTASGGGSEVSANAAKALSKDFMHLLVTQL
                     QHQDPLQPMQDRELIAQLAQLSSLESLQRIEELTARDHSLNQLSQAAGLIGKVITATT
                     ADGAVSGKVSAAMVRPGTILSQTPR"
     gene            complement(5085..5227)
                     /locus_tag="PLY56_06535"
                     /pseudo
     CDS             complement(5085..5227)
                     /locus_tag="PLY56_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
BASE COUNT          937 a         1598 c         1845 g          847 t
ORIGIN      
        1 ggaatccccc gccacgccgg aggaagcggt cgagccagag ccagagcccg aggcggagcc
       61 cgaggagcca gaggaaaccg agcgcccgcc agagatactg ccgacgatct ccccgacgga
      121 agagcggctg cgccgcttgg cggccgaaga tccggagttc gtggcgcggc tcctcagaag
      181 ctggcttatc aaccagggga agcgctgacg tatagagcgc ctgtgtgagg ggcatctgga
      241 gttgggcctg aatggactcg agagtgcccc tcctttggag aggcgcgaag aaagggatct
      301 gccgtggatc cggtgatcgg aatggaagag cgcccggtgc cggtggcaca gctgccggct
      361 gtgcgcaagg cgtcgattgt gctgatctcg ctggggcaag agctttccgc caacgtcttg
      421 aagcacctca accgcgatga gatcgagatg ctgacgctgg agatcgcgcg catcaagcac
      481 gtctctcccg atctgcgcga cgcggtgctc gccgagttct cccagatgat cgtcgcccag
      541 gaatgcatca tcgagggcgg cattgagtat gcccagggtg ttctgcagca ggcactcggc
      601 aaagagacgg ccagtgagat catttcgcgc ctggtgacga tgctccaggt gcagcccttc
      661 gactcggtca agagcgtcga cccgacgcaa ttgcttcagc tgctccagaa cgagcacccg
      721 cagacgatcg cgctggtgct ggcacacctg ccaccggaga catcggctat ggtcctctcc
      781 ggactgcccc agaagctgca ggcccaggtc gccatccgga tcgtgggtat ggatcgcgcc
      841 tcgccggagg tggtgcgcga agtagagggc atcctgcagg ctcgcatcac cgcgacgggg
      901 acgcaggact tcgataaggc gggtgggccc tccgcgctgg cagatatcct caaccgcgcc
      961 gaccgctcga cggagcgcgc gatcctggac gacctgatgg agaacagccc cgagatcgcc
     1021 gaggaagtga agaagcgcct cttcgtcttc gaggacatca tcaccctggg tcggcgcgat
     1081 atccagctcg tgttgcgcga agtggacggc aaggacctgg cgttggccct caagggcgct
     1141 ccggagaacg tgcgcaacgt gatctacgag aatatgtcgg aacgcgctgc gcaatcgctg
     1201 cgtgaagaga tggagttcat gggagccgtg cgcctgcgtg acgtggaagc ggcccaacag
     1261 aagatcgtct cggtgatccg tgccctcgaa gaggccggcg aggtcgtgat ctcgcggggt
     1321 gaggagaatg agaccattat ctagagtgct caaagccccc taccgggatc acgcgccgca
     1381 tgtgatcagc gctcccgtct ttccctcctt cgataccgag ttgctggtgc ccgacgagga
     1441 tgaggcggcg gagcccgagc tcttgtgcga ggaagaggcc cccatcgaag agccgccgga
     1501 gccggagccg gagccgcccc cggagcccac ggcagaggtg gatgaagagg aagaggaaga
     1561 ggagccggcg ggcccgagcc ccgaagaaga ggcggccgca atccgtgccg aggcggagca
     1621 gatgctggcc gaggcgaggt cgcaggttgc agagtttctg cgcgaggccc agcagcaggc
     1681 acaggcgatg gaggcggagg cgcgcgagcg cggctttcgt gaggggatgg aggccgctca
     1741 ggatgctcag cgccggctcc tcgcggatct ggaggcgctg cgcgcggcgg tgcaggccga
     1801 gcgagacctc ttcttcgaga acctggagcc cgaggtggtc cgtctcgccc tggccattgc
     1861 ggagaagatc atccgccagg aggtggatct gcatccggag attgtcgtag gggtggcgcg
     1921 cgccgccctc cttcagctga aagatgctca aaccgcgaga gtgagcgcgc acccctccag
     1981 cgtggacgcg ttgcgccagc accttgccag cagcctcccc ggcggtccat ccgagatctc
     2041 tatccatgcc actcccctgg tcagccccgg cggatgcatg atcgattctg atcgggggag
     2101 cgtcgatgcg cgcctggaga cgcagatgga gcggatcgcc acagcgcttc aggagggaga
     2161 caccggatga tcgccgatgg ccggccgatg cttgagaccc ccggattcct gggggatgag
     2221 ggtcgcgagg gagaggccgg gccagggtac acccctcacg gatcagccct cgaggctcgt
     2281 tgtccaaaaa tcgatctatc gccctatatg agccggctgg gtgaggtcgc ctctttcctg
     2341 ctcagcgggc gcgtggtgcg ggtggtgggc accgtcatcg agtccatcgg ccccccctgc
     2401 gctatcggag aggtctgtcg catccacacg gcggatggca aggagatcta tgccgaggtc
     2461 gtcggcttca aggacgagcg gctgatcctg atgccgatgg atgaggtggg caacctggag
     2521 cccgggagca cggtggtgac caccggagct ccgctgcgcg tgcccgtggg cgagagctgc
     2581 ctggggcgga tcttcgatgg ccttggccgg cccatcgaca acggcggccc gctccttggc
     2641 accacgctcc gtgcggtcca cgcctctccg ccggatcctt tgactcgcga gcgcgtgaat
     2701 cagcccctgg tgacagggat ccgcgccatt gatggcctgc tcacgtgcgg gcgcggccag
     2761 cgcatcggca tcttcgctgg aagcggcgtg gggaagagta ccttgctggg atgcatcgcc
     2821 cggcattcaa cggcggatgt caacgttatc ggcctgatcg gagagcgtgg ccgggaagtg
     2881 cgcgagttcc tggagcgcga cctgcgcgaa gagggcctgc ggcgctccgt ggtggtggtg
     2941 gcgacttcgg accaatcgcc tcttcagcgc ctgcgcggcg ccgcggtggc cacaacattt
     3001 gccgagtatt tccgggatca gggcctggat gtggttctga tgatggattc gctgacccgc
     3061 tttgcctggg cgcagcgcga gattggcctg gccgccgggg agccgccggc gacacgtggc
     3121 tacacccctt cagtgttcgg cgcgcttccg aggctcctcg agcgcgccgg cacggcatcg
     3181 cgcggctcga tcaccggatt ttactccgtc ctcgtggatg ccgatgatat gaatgatccg
     3241 atcgcggata ctgtgcgcgg aattctggac ggtcacatcg tcctctcgcg ggatctcgcg
     3301 cggcagggac actatccggc gatcgatgtg ctctccagcg tgagccgcat catgagtgag
     3361 gtgaccgagg aagaccaccg acaggctgcc acgcggatgc gcgatctcct cgctgtttac
     3421 cgcgaggcag aggacctgat caacatcggc gcctatgtgg ctggaagcaa cccgcggatc
     3481 gatgctgcca tcgcgcacat cgacgcgatc cgccagttcc tgcggcagga tgctcaggcg
     3541 atctccccct atgaggaaac gttgcggctg ctggcgagtt gcgtggggca gaatggagca
     3601 gagtagggtg aaaccttttg tcttcaacct gcagacagtt ttggaggtgc gtcgcattcg
     3661 ggaagagcaa gcccgggcag atctggcggc ccttcgctat cgggagaagc aggaggagga
     3721 gcgaatcgcg cgcctgtgcc agcagcggca ggatgtacac cggcgcctcg tcgatggcaa
     3781 cgctcccttg agtggctccc aagtcctcgc cgcggccagc acggagcgct cgctggatct
     3841 ggccatccac gattcccagc ggcagaagga agagatcacc cgccagcgcg aggtgaagct
     3901 ggaacagacg cggatggtga tcacgcagcg caagaccgtc gagcttcttc gtgagaaggc
     3961 ctggggacgg cacctgtatg aggccgcggt tgcggcagaa cgccaggtgg atgagttgtg
     4021 ctcccgcagg gtggaagagc gcgccgggta gagagcggga ggagcagaat gctggagaat
     4081 ctgcaacgtg tcgaggcgcg gatcgccgtg ttggagcagc ggctctcggc gatctcggct
     4141 ccgcccgcgg aggcaggcgc ctcgccggcg tcgagcttcg cgcaggcgct cgcggcggca
     4201 tcgcgcagtg gggaaagcgg aacgcttccc ccagccccgg tggcgccgat ggcggcagcg
     4261 ccgctccggg acgcgccggc gaatctgccc ccgccgccgc gctgggtggg ccatgcgccg
     4321 gatgcggatg acgcggagga ggcggttcgc cgcgcggcgc gccggcacgg ggtggatcca
     4381 agcctcgccg ttgccgtggc acgggcggaa tccgggttca acccgcttgc cgtcagctct
     4441 tccggagcgc ttggcctgat gcaactgatg cccggcacgg cccgctccct cggagtgagc
     4501 gacccgtttg atgtggagca gaacgccgat ggcggggtgc ggtatctggc cgagcagctg
     4561 cgccgcttcg gcgatgtgcg cctggcgctt gcggcctaca acgctggtcc gaaccgtgtc
     4621 gcccgctatg gcgaggtgcc cccgatcccg gagacacagc ggtatgtcga gcgcgtgctg
     4681 gagtatcaac agcagtatcg cgcgagcggc actccgtgat gcgctatcca ggggcaagct
     4741 gagagcaaga ggctgttgac cgataggaga gagacgatgc gtatcgatgg cacgaggagc
     4801 accgcgagcg gcggtggaag cgaggtatcc gcgaacgcgg ccaaggcgct gagcaaagat
     4861 ttcatgcacc tcctggtcac gcagctgcaa caccaggatc cgttgcagcc gatgcaggac
     4921 cgcgagttga tcgcccagtt ggcccagctc tcctcgctgg agtcgctgca aaggatcgag
     4981 gagctcaccg cgcgagatca ctcgctgaat caactctcgc aggccgcggg actcatcggg
     5041 aaggtgatca cggccacgac tgcggatggg gcggtctccg gcaaggtgag cgccgcgatg
     5101 gtgaggcccg gcacgatctt gtcgcagacg ccgagataga cgaccgaatc gaacatgttg
     5161 tgcgagaggc cgacgcccgc cgccatggcg atcaagctgc ccccgttgct aaagcctagc
     5221 gccactt
//
LOCUS       DAOSCV010000209         5210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10015_length_5210_cov_2.830068, whole genome shotgun sequence.
ACCESSION   DAOSCV010000209 DAOSCV010000000
VERSION     DAOSCV010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10015_length_5210_cov_2.830068"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            793..1158
                     /locus_tag="PLY56_06540"
     CDS             793..1158
                     /locus_tag="PLY56_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ21173.1"
                     /translation="MLSERERPAKRPDSSLLRIRLLDEGEGICRVECAGEIDIQTAPR
                     FGEELAKALEKAPCRVIVDLRQVSRIDSVGVRHLLDARAGVAVGSKLEVQATDPRVRM
                     MLEIAGVERERARSIPGVR"
     gene            1210..1626
                     /locus_tag="PLY56_06545"
     CDS             1210..1626
                     /locus_tag="PLY56_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21174.1"
                     /translation="MSPEALQCPSCGSRRLFTCALCGKMVDAPLNPPQLLGNGAVACI
                     RHAPWARCSTCGRVGTYTEVTTTGWVYLGDRSAVWLLRRYPGATAGALRVRATQRWLC
                     PACNEIYRQESQSSKARLAAGVAAVWAALREALTRT"
     gene            1678..2094
                     /gene="rnk"
                     /locus_tag="PLY56_06550"
     CDS             1678..2094
                     /gene="rnk"
                     /locus_tag="PLY56_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOJ21175.1"
                     /translation="MSDKAGQRDIYVTEEDRARLLEMLRRDPPASEGLRTLAAELDRA
                     RVVTAAEVPPDVITMDSQVQLEDLDTGQEMVCTLVFPEDADASQGKISVLAPIGMAML
                     GYRVGDVFEWQVPSRKRRLLVKAVLYQPEASRVASR"
     gene            2448..3158
                     /locus_tag="PLY56_06555"
     CDS             2448..3158
                     /locus_tag="PLY56_06555"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ21176.1"
                     /translation="MEARHELIRMLVPGCRAILAYAGRTLPAIICSAYDAGFRVQIHA
                     VDVSERPFAVGERVAFLAQNDRGLAFFDTRILASYAGNPACLDLEWPSRFEWRPRREH
                     TRIQERIPVTVIEPTRHGQPGPSRSGHTLDISVSGIQLFLAGDAGKNLEPGRQVDLLL
                     GLPDGWPPLEATAMVVRTLAATVDDTPGWALGLRFTAASEQAWDRIARHIQVLMRRAN
                     DRSRGSVPDLRRAAHHER"
     gene            complement(3311..3928)
                     /locus_tag="PLY56_06560"
     CDS             complement(3311..3928)
                     /locus_tag="PLY56_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOJ21177.1"
                     /translation="MSRMSWVYSEKVKDHFMNPRNILEDEESFEADGRGVTGNVKCGD
                     EMLVLIKVDKERGIITDCRWRTYGCASAIASTSVLSEMVKGMPLDEAYKLGAKDVNRA
                     LGGLPEHKVHCSVLGDKALRAAINDYYARNGMHDKIRQEEQRVVCQCMGVTDLEIESA
                     VLDGARTYYEVQERTKVGTVCGGCEDEVKQLIEFYTNKHFAEDAA"
     gene            complement(3925..5115)
                     /locus_tag="PLY56_06565"
     CDS             complement(3925..5115)
                     /locus_tag="PLY56_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ21178.1"
                     /translation="MPERQVYFDNNATTPLHPAVKKTLVEAMDLYGNPSSLHAAGRLA
                     RNAVEEVRERVAEFIGATPDEVIFAGSGSEANNTVLSIPTCPSKGCDCVMHESTEIVT
                     SVIEHPCILETAKCLGERGVSIRYIGVDQYGRVDPEELRRSITERTRLVSIMMANNEV
                     GTIQEIKKLAAIAHERGALFHTDAVQAVGKVPVNVQELGVDFLTLSGHKIYGPKGVGA
                     LYIRKGAPFCPLIRGGHQEKGRRAGTENTLGIIGLGKAIELRKEEMEEEEKRLRAMKA
                     ALRQGIEEKIPDVHFNGHPTESLPGTLNVSFDGAEGEALLLYLDLEGIAVSTGSACAS
                     GSLEPSHVLLAIGCPVERAHGSIRISLGRENTMADVERMLEVLPKVVERVRRMSTAYG
                     REVA"
BASE COUNT          883 a         1706 c         1640 g          981 t
ORIGIN      
        1 cgctcgcaag cagcgcggtg atttatcgcc gcgcgacggg ttgcgcaatg cctccgaggc
       61 ttcgaactcc ttatgtcgga atctcagctt gcccctcggc gggattgaca ggcccgggtt
      121 tggatggtag catagggcca tgggaaggct cctgacgagg ttggccggtc aaggtatcgg
      181 tggcagagcg gctggatggc gcgtctggtt gccggtggca ttcgcggcgc tcctcttggc
      241 ggtgggcctt tcctcgcatg ctcccgtcta cgggtgcatg cgccctgccc tgatcgctgc
      301 aggcacccca cgcctgcagc cctgtcccac actctctaag catccgtccc atagcggccc
      361 cgaggcctgc tgcgaccggc aggatagtcc ggtccatgcc acacgatcgc actcgcgggc
      421 ggcgcgacct atctgcctgc ttcgcgccgg gcgtgccaca cgctggtgta gcctcggcac
      481 gccgatttcc catgcttgcc attcgcggtc tgggccgcag cacacccttc cccatctccg
      541 ggccgagtac agtgcctgga atggtatcgg cagcccgttg cgatagcaca ctgccatccc
      601 actactgtgc ccgcctcgct ggcccgggca actctcatcg ttgcactgcc cctgcatgat
      661 ccaccagggg cttgcctggc aggagcggca caggtccttt ccgttttccc gggcactctc
      721 ttcaccagcg attaccatga ggggagcgcc ttcaaacagc tatgcccagg tggcggcaga
      781 gaggattgat cgatgctgtc cgagagggag agacccgcaa agcgcccgga tagctctctc
      841 ctgagaatcc gtctgcttga cgaaggggag ggcatctgcc gggtggagtg cgcgggggaa
      901 atcgatatcc aaacggcacc tcgcttcggt gaggagctgg ccaaggcgct cgagaaagcg
      961 ccttgccggg tgatcgtgga cctgcggcaa gtgtctcgaa tcgacagtgt aggggttcgt
     1021 cacctgctgg acgcccgtgc gggggtcgcc gtaggctcca agttagaggt ccaggccacg
     1081 gacccgcgtg ttcgcatgat gttggagatc gcgggagtgg agcgcgagag agcccgctcc
     1141 attccgggag ttcgctgatt gaggatgccg cgcgacgcga ggatgcggtg tcagatctgt
     1201 ggagcggccg tgagcccaga ggcgctccaa tgcccctcgt gtggctcgcg ccggctcttc
     1261 acgtgcgccc tatgcggtaa gatggtagat gcaccgctga atccgccgca actcctgggt
     1321 aatggagcgg tcgcctgcat ccgacatgcc ccctgggcca ggtgttccac ctgcggcaga
     1381 gtgggtacct acacggaggt gacgacgacc ggatgggtct acctggggga ccggagtgcc
     1441 gtctggcttc tgaggcgcta ccccggggcg acggccggag cattgcgtgt gcgggcgacg
     1501 cagcggtggc tctgccccgc atgcaatgaa atctatcgtc aggagtctca gagcagcaag
     1561 gcccgcctgg cagcgggagt cgcggcggtg tgggccgcgc tgcgcgaggc tctgaccaga
     1621 acgtgaggct ggggatccct gcgccggatg acggcgcaac ccggcaggag agaagagatg
     1681 agtgataagg ctggtcaaag agatatctat gtaaccgagg aagaccgggc gcgcctgctt
     1741 gagatgctgc gccgggatcc ccccgcgagc gaaggcttgc ggaccctcgc ggccgagctc
     1801 gatagggccc gggttgtcac cgccgcggag gtgcctccgg atgtcatcac catggactcg
     1861 caggtgcagc tggaagacct ggataccggc caggagatgg tctgcacgct cgtcttcccg
     1921 gaagacgcgg atgcatcaca gggcaaaatc tcggtgttgg cccccatcgg catggcgatg
     1981 ctcggctacc gggtgggcga cgtcttcgag tggcaggttc catcgagaaa gagacggctg
     2041 ctcgtcaagg cggtcctcta ccagccggag gcatcgagag tcgccagcag gtaagaccag
     2101 cgagcggaga gcgagccccc aatgctcaga aaggggtcga taccgaactg tccgccctcg
     2161 ccgcgccggt cgcggtgggg gcacgttttt ctctgccagt aaccctgtgt ccaccccgga
     2221 tgcctcctgc ttgatccgcc gccccttcaa gacccgggtg gggaggaacc ggccgctaca
     2281 gcgcgaactt caaggcagct tgcactcgga gttgggattc tgttccgctt cctgcgatta
     2341 tgctgtctat tgggccagag acgggatgat gaggaagagg atgccctgcc acaggcctct
     2401 gagtgtggaa gtaatctatc gcgcgatcgc gtgaaaggac gggaccgatg gaggcccgcc
     2461 acgaactgat tcgtatgctg gtgcccggct gccgggcgat cctggcctac gcagggagaa
     2521 cgcttcctgc catcatttgc tctgcatacg atgcggggtt tcgcgtgcag atccacgcag
     2581 tggatgtttc cgaaaggccc tttgctgtgg gcgagcgcgt ggcattcctc gctcagaatg
     2641 ataggggtct ggcgttcttc gacacgcgta tcctggcctc ttacgccggc aaccccgcct
     2701 gtctggatct ggaatggccc tcgcgcttcg agtggcgccc gcgccgcgag cacacgcgca
     2761 tacaagagcg gatccccgtt accgtgatcg agcccacgag gcatgggcaa ccgggcccat
     2821 cgcgctccgg tcataccctc gatataagcg ttagcggcat ccaactcttc ctggcgggag
     2881 atgccggcaa gaatctcgaa cctggccggc aggtggatct gctcctgggg ttacctgatg
     2941 ggtggccccc cctggaggcc accgcgatgg tggtgcgaac gctggcggca accgtagatg
     3001 acacacccgg ttgggcgctg gggctacggt tcaccgcagc gagcgagcaa gcgtgggaca
     3061 ggatcgcccg ccatatccag gtcctgatgc gccgggccaa tgaccgatcc cggggctcag
     3121 tgccggacct gcggcgagca gctcaccatg aacgttaagc ccgggaagcc cctccggtac
     3181 cggaggggct tcccaatcct tcccgcggtg ctatcaccgt agcgagtggt attctgctgc
     3241 acgtcgccac agatcagcgg gagatatcgc cacggccggg gtcttagcca gccggtttgg
     3301 ccggcatcgc tcaggcggcg tcttcggcga agtgcttgtt cgtgtagaac tcgatgagct
     3361 gcttcacttc atcctcgcac ccgccacaca cggtgcctac cttggtgcgc tcctgaacct
     3421 cgtagtacgt gcgcgcccca tcgagtacgg ccgactcgat ctcaaggtcc gtcacgccca
     3481 tgcactggca gacgacgcgc tgctcctcct ggcggatctt gtcgtgcatc ccgttgcgcg
     3541 cgtagtaatc gttgatcgct gcgcgcagcg ccttatcgcc cagcacggag cagtggacct
     3601 tgtgttccgg caggccgccg agcgcgcggt tcacatcctt cgcgccgagc ttgtaggcct
     3661 catccagggg catccccttc accatctccg agagcacgga ggtgctggca atcgcgctag
     3721 cgcagccata cgtcctccag cggcaatcgg tgatgatccc gcgctccttg tccaccttga
     3781 tcaggacgag catctcatcg ccacacttca cgttgccggt caccccgcgc ccatccgcct
     3841 cgaagctctc ctcgtcctcg aggatgttcc gcggattcat gaagtggtct ttcaccttct
     3901 cggagtagac ccagctcatc cggctcatgc gacctccctt ccataggccg tggacatgcg
     3961 ccgcacccgc tcgacgactt tgggcaacac ctcgagcatg cgctcgacgt ctgccatcgt
     4021 attctcacgg cccaagctga tccgaatcga tccgtgcgct cgctcgacag gacacccgat
     4081 ggcgaggagc acgtgcgaag gctccagcga gccggatgcg cacgcggaac ccgtcgagac
     4141 ggcgatgccc tccagatcga ggtagagaag gagcgcctcg ccctccgcgc catcgaagga
     4201 gacgttgagc gtgccgggaa gcgactccgt ggggtggcca ttgaagtgca cgtcgggaat
     4261 cttctcctcg atgccctggc gcagcgccgc cttcatggcc cgcagccgct tctcttcctc
     4321 ctccatctcc tccttgcgca actcgatcgc cttgcccagg ccgataatcc ccagcgtgtt
     4381 ctcggtgccg gcgcggcgtc ccttctcctg atgccctccg cggatgagcg ggcagaaagg
     4441 tgcccctttg cggatgtaga gcgcacctac ccccttgggt ccgtaaatct tgtgccccga
     4501 aagcgtgagg aaatcgacgc cgagctcctg gacgttcacc ggcaccttgc cgaccgcctg
     4561 caccgcatcc gtgtggaaca gcgcgccacg ctcgtgggca atcgccgcta gcttcttgat
     4621 ctcctggatc gtgcccacct cattgttcgc catcatgatc gagacgaggc gggtgcgctc
     4681 ggttatcgac cggcgcaact cctccggatc gacgcgaccg tactgatcca cgccgatata
     4741 gcggatgctc accccgcgct cgcccaggca cttggccgtc tcgaggatgc acggatgctc
     4801 gatcaccgag gtgacgatct cggtcgattc gtgcatcacg cagtcgcatc ccttcgaagg
     4861 gcacgtgggg atcgacagca cggtgttgtt cgcctccgag ccactgccgg cgaagatcac
     4921 ctcgtcgggg gtcgcgccga taaactcggc gacgcgctcg cgcacttcct cgaccgcatt
     4981 ccgggccagc cttcccgcgg catgaaggct ggacggattt ccataaagat ccatcgcctc
     5041 caccagcgtc ttcttcaccg cagggtgaag cggggtggtg gcgttgttat cgaaatagac
     5101 ttgcctctcc ggcatcaaat cctccattac gaaccatact ttactcgggc gctgcatccg
     5161 cccccggtgc gcagcccagc gccgccgctc ttcgcctcga ccccgacaac
//
LOCUS       DAOSCV010000210         5191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10057_length_5191_cov_3.373832, whole genome shotgun sequence.
ACCESSION   DAOSCV010000210 DAOSCV010000000
VERSION     DAOSCV010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10057_length_5191_cov_3.373832"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLY56_06570"
     CDS             complement(<1..249)
                     /locus_tag="PLY56_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ21179.1"
                     /translation="MSLADAALLREFIQHVPAAVAMFDRQMRYIFVSERWMEDYRLGD
                     QEIIGRTHYEVFPEIPERWKELHRRALACEVVRCDEDRF"
     gene            683..1582
                     /locus_tag="PLY56_06575"
     CDS             683..1582
                     /locus_tag="PLY56_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ21180.1"
                     /translation="MTKPWEAERVVDPELARRLIRAQAPGIAAEQIELLGAGWDNTVY
                     RVDGEWVFRFPRREIGARLIAIELAILPRIAGRLPAPIPNPVWAGEPTPDFPWPFAGY
                     RYLPGEPASHLDLDDETRVALAEPLGQFLAALHALPIAELVALGLPPDTLGRFDLDRR
                     IPQAQEQLDRALHLGLISSLEPYLPLMEGLPRGPRPTCLVHGDFYARHLLLCDRRLCG
                     MIDWGDLHAGDPAVDLMVLAAFLPACARPAFLASYGPIEDETLRLARFRALYHTLALL
                     LYAHDLGDAPLLREARRALGYLS"
     gene            complement(1603..2049)
                     /gene="arfB"
                     /locus_tag="PLY56_06580"
     CDS             complement(1603..2049)
                     /gene="arfB"
                     /locus_tag="PLY56_06580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOJ21181.1"
                     /translation="MVENEASGVLQITPEVSVRESDLQFRTSRSGGPGGQNVNKVETR
                     VELLFDTACLGPLSDEQRERALADLRPWLGSDGVLHLVSQRHRSQRRNREEVIERFVS
                     LLQWALRPRVARKRTRVPRAAREARLAEKRHRARVKQQRRPVRDID"
     gene            2188..4149
                     /locus_tag="PLY56_06585"
     CDS             2188..4149
                     /locus_tag="PLY56_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21182.1"
                     /translation="MRSSHVLEKTHPSEAPSEEPAQEEAHGGGRRAWVIGLLGVLFLC
                     LAAPYTDLTLMGSELAGNHLPLGAVCVFLALVLGSRLLRRVFPRWRELSARELLLAFA
                     MMLVAAGLPTFGLAAYLFPAASAPVYYATPENRYAEVLLSYVPAWLIPQDADAVRHFY
                     LGLPPGATIPWGAWVRPLAAWSALALAAYLVILSLGVLMSDQWIRRERLSYPLMQLPL
                     EVTTQKRWNWRDPLLLLGMAVPMALHTLNGLHSVAPEFPEIRLKNVPFLDLGTDKPLS
                     YLRSSINIYFCVIAVAYLLPAEVSFSIPFFFFFNKVQYMAAYMHGSDLAFGKVVEAQY
                     VGAFLVFVFAGVWAARPHLREVWRSALGQHAKVGGKEYRWALLGLLAGAIGMVAWLAV
                     AGVRPVVGLAVVVIFIAICIGLGRIVVESGALFAKATQMRPLTAFDYLTGTTWLRPAD
                     VPVVGLAQYVFFYDLKVFLMPAVAHALKLGDAAGTRRRTMLGAMAAAILLGMVASYAS
                     LLWMAYHHGAIKMNSWFFAIGPRSHLDSFVSTTTAPRAFEASKLATVAAGGAFAWFLA
                     AMRQRFFWWPFHPVGYVLAYSSETTRIWFPFLAGWLCKVLLLRGGGLRLYRSGCRFFW
                     GLMIGEFAAAALWLVVSTATGIEGYKIFP"
     gene            4414..>5191
                     /locus_tag="PLY56_06590"
     CDS             4414..>5191
                     /locus_tag="PLY56_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ21183.1"
                     /translation="MHNPKWCLFYDVHTQPALPDVGEGFDPEAWADRFQRCGVDYVVF
                     HARCNMGMAYYNTKIGIRHPSLKYDLFGAFVEACQKRNIAVTAYVNVGLSHEEGLLHR
                     DWLVLNPDGTTYLPNRMGNFMRMMCPNTGYGDHVVAMCREIVENYPVAGLFLDCMHQH
                     PCVGVECIREMKQRGIDWEDPRQLREFANLSRVRIAQRIADAAKSVRPDLLLYFNGVS
                     YEYQQEIGTYLEYECLPTGGWGYEALPLYGRFLGTLGKPLL"
BASE COUNT          798 a         1764 c         1652 g          977 t
ORIGIN      
        1 aaagcgatcc tcatcgcagc gcaccacctc gcacgccagc gcccgccggt gaagctcctt
       61 ccaacgttcg ggaatctctg gaaagacctc gtagtgtgtg cgcccgatga tctcttgatc
      121 gccgaggcgg taatcctcca tccaccgctc gctgacgaag atgtagcgca tctgccggtc
      181 aaacatcgcc accgcagcgg gcacgtgctg gatgaactca cgaagcagcg ccgcatccgc
      241 cagagacacg ccgcctgggt gcatccccct tcgcatccgg cggccctgcc gcgatggcga
      301 actccaccgt ctccgtggcg atctcctcag acgacgcatc gctccccttc tcggccatgg
      361 cccgcatcgt gttcccatca gatggccggc aagcctgaac gcggcacgcc cggcatccca
      421 tacccccgtt tcccattctg gcatgggata ctccatgagg cgatccccat atggaaaaac
      481 gcgtccggtc gccatcctgc cagattcgcg gggcggcctg aatccgaccg gggtagctca
      541 tctgccctga ccgcgaggag tttctctacc aggtgcaggg gcccctgcaa aatgagccga
      601 aaagagaatc cgtgcagcac cgaaagggca tttcagtaga atgggcaagt gaaggcgcgg
      661 atgcgcgctt gcgggaggag cgatgacaaa gccgtgggag gcagagcggg tggtggatcc
      721 ggaactggcc cggagattga tccgcgcgca agcgcccggg atcgcggcag agcaaatcga
      781 gttgctgggc gcgggctggg acaacaccgt ctaccgcgtc gatggcgagt gggtgttccg
      841 ttttccgcgc cgggagatag gcgcccgtct gattgccatc gaattggcga ttctcccccg
      901 gatcgccggc cggctgccgg caccgatccc caacccggtg tgggcaggcg agcccacgcc
      961 tgacttcccc tggccgtttg ccggctaccg ctatctgccg ggcgagcccg ccagccacct
     1021 cgacctggat gatgaaacgc gcgtcgctct cgcggagccc ctggggcagt ttctggcggc
     1081 gctccatgct ctcccgatag cggaactggt ggcccttggc ctcccacccg acaccctcgg
     1141 ccggtttgac ctcgaccggc gtatcccaca ggcgcaggaa cagctggacc gcgcgctcca
     1201 tctggggctc atctcctctc tcgagcccta cctgcctctg atggaggggt tgccgcgagg
     1261 gccgcggccg acctgcctgg tacacggcga cttctacgcg cgccacctgc tcctgtgcga
     1321 tcgccggctc tgcgggatga ttgactgggg cgatctccac gcgggcgacc cggcagtcga
     1381 tttgatggta ctcgccgcct tcctgccggc ttgtgcccgg ccagcgttcc tcgccagcta
     1441 tggccccatc gaggacgaaa cgctgcgtct ggcgcgcttt cgggcgctgt accatacgct
     1501 ggcgctcctc ctctacgcgc atgacctggg ggacgctcct ctgctacgcg aggcccgccg
     1561 cgcgctcggc tacctgagct aagcgaggaa ccagcagccg gctcagtcga tatcgcgcac
     1621 tgggcggcgc tgctgcttga cccgcgcgcg gtgccgcttc tccgccaggc gtgcctcgcg
     1681 ggcagcgcgc ggcacgcggg tgcgcttgcg ggccactcgt ggccgcaacg cccattgcag
     1741 cagcgagacg aagcgctcga tcacctcctc acgattgcgg cgctggctgc ggtggcgctg
     1801 cgacaccagg tgcagaacgc catcactccc cagccagggg cgcaggtcgg caagggcgcg
     1861 ctcgcgctgc tcgtcggaaa gcggccccag gcacgcggta tcgaagagca gctccacgcg
     1921 cgtctccacc ttgttgacgt tctgcccacc cggaccgccg cttcgcgatg tgcgaaactg
     1981 aaggtcgctc tcgcgcaccg atacctccgg cgtgatctga agcacgccgc tcgcttcgtt
     2041 ctccaccatg tggtcctcta ttccccctcg tggggatgcc ccgatttctt gttccgaacg
     2101 accagcaggc ttccggccat tcaggggcga agttagcttc cagcgttctg gcgccgttag
     2161 gctccggcgc atcgatggag ggctatcgtg cgttcctcac acgtgctaga gaaaacccac
     2221 ccgagcgagg caccctcgga agagcccgcc caggaggagg ctcacggagg aggtcgccgt
     2281 gcgtgggtga tcgggcttct gggagtactt ttcctgtgtc tggccgctcc ctataccgac
     2341 ctcaccctca tgggttcgga gctggcgggc aaccacctgc ccctgggcgc agtttgcgtc
     2401 ttcctcgctt tggtgctggg gtcgcggctg ctgcgccggg tcttccctcg ctggcgagag
     2461 ctctctgcac gggaactcct gttggccttc gcgatgatgc tggtcgcggc ggggcttccg
     2521 acgttcggcc tggccgccta cctcttcccg gccgcctcag cgccagtcta ctacgccacg
     2581 ccagaaaacc gctacgcgga ggtgctcctc tcctacgtgc cggcctggct gattccccag
     2641 gacgcggacg cagtgcgcca cttctacttg gggttgccgc cgggcgcgac gatcccctgg
     2701 ggagcgtggg tgcggcccct tgccgcctgg tcagcgctcg cgctcgctgc ctacctggtg
     2761 atcctgagcc tgggcgtact gatgtcggat caatggatcc ggcgcgagcg cctctcgtat
     2821 ccgttgatgc aactcccgct ggaggtgacg acgcagaagc gttggaactg gcgcgacccg
     2881 ctcctcctcc tcggaatggc cgttccgatg gccctgcaca cgctgaacgg gctacactcg
     2941 gtcgccccgg agtttccgga gatccgcctg aaaaacgtgc ccttcctcga tctgggaacg
     3001 gataagccgc tctcctacct gcgctccagt atcaacatct acttctgcgt gatcgccgtg
     3061 gcctatctgc ttccggcgga ggtctccttc agcatcccat tcttcttctt cttcaacaag
     3121 gtgcagtaca tggcggccta catgcacggc tccgacctgg ccttcggaaa ggtggtcgag
     3181 gcccagtatg tgggcgcgtt cctggtgttc gtcttcgcgg gcgtctgggc tgcgcgccca
     3241 cacctgcgcg aggtgtggcg cagtgccctg ggccaacacg cgaaggtcgg gggaaaggag
     3301 tatcgctggg cgctgctggg cctgctggcc ggtgcgatcg ggatggtggc gtggctggca
     3361 gtggcgggag tgcgccccgt cgtgggcctg gccgtcgtgg tgatcttcat cgccatctgc
     3421 atcggtttgg ggcgtatcgt cgtcgagagt ggggcgctct tcgccaaggc gacacagatg
     3481 cgcccgctca ccgccttcga ttacctcacg ggcacgacgt ggctgcgtcc ggcggatgtg
     3541 ccggtggtcg gcttggcgca atacgtcttc ttctacgact tgaaggtctt cctgatgccg
     3601 gcggtggcgc acgcgctgaa gctgggagat gccgccggca cccgccggcg gacgatgctg
     3661 ggcgccatgg ccgcggccat cctcctgggg atggtggcgt cgtacgcgag tctcctctgg
     3721 atggcttacc atcacggcgc catcaagatg aactcgtggt tcttcgccat cggcccacgc
     3781 tcgcacctcg acagcttcgt cagcaccacc acggccccac gcgccttcga ggcatcgaag
     3841 ctggccaccg tcgccgcggg cggcgccttt gcctggttcc tcgccgcgat gcgccagcgg
     3901 ttcttctggt ggccgtttca tccggtgggc tacgtcctcg cctactcttc cgagacgacg
     3961 cgcatctggt tcccgttcct ggcgggctgg ctgtgcaagg tgctgctgct gcgcggcggt
     4021 ggactccggc tctaccgctc tggctgccgc ttcttctggg ggttgatgat cggggagttc
     4081 gctgcagccg ccctgtggct ggtggtcagc accgccaccg ggatcgaggg ctacaagatc
     4141 ttcccgtagg atcccttgtg gccatgcagg agccgcagga agctctgaca acggtcgaga
     4201 aggctaagga aacctggctg caatcggtgg gcgccgtggt ggggggcagc aggcgagcag
     4261 ataccggttc aggaggcacg gtcgcaggca caagggcagc ccatcagacc acacgcactg
     4321 gcgatgggtt gccgtgccag cgggcctcgc gcgcctctgc tcgccacccc tgaagtgaca
     4381 ggccgcggat ccggaagccg gatggaggga atcatgcaca acccgaagtg gtgtctcttc
     4441 tatgatgtcc atacgcaacc cgctttgccg gacgtaggcg agggcttcga cccagaggcg
     4501 tgggcggatc gtttccagcg ttgcggcgtc gattatgtcg tcttccatgc tcgctgcaat
     4561 atgggcatgg cctactacaa cacgaagatc ggcattcgcc acccctcgct caagtacgat
     4621 ctgtttggcg ccttcgtgga ggcgtgccag aagcgcaaca tcgccgtcac cgcctatgtg
     4681 aacgtggggc tcagccacga ggagggcctg ctccatcgcg actggctcgt gctcaacccc
     4741 gacggcacca cctacctgcc aaaccgcatg ggcaacttca tgcgcatgat gtgccccaac
     4801 accggctacg gcgatcacgt cgtggccatg tgccgcgaga tcgtcgagaa ctacccggtg
     4861 gcgggcctct tcctcgactg catgcatcag cacccctgcg tgggcgtgga gtgcatccgc
     4921 gagatgaagc aacgcggcat cgattgggag gacccccggc aactccgcga gttcgccaac
     4981 ctctcgcgcg tgcgcatcgc ccaacgcatc gccgacgccg cgaaatcggt tcgccccgac
     5041 ctgctcctct atttcaacgg ggtgagctac gagtatcagc aagagattgg cacctacctg
     5101 gagtacgagt gccttcccac cggcggttgg ggttacgagg cgctcccgct ctacgggcgt
     5161 ttcctgggca cgctcggcaa gccgctcctg a
//
LOCUS       DAOSCV010000211         5161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10114_length_5161_cov_4.498629, whole genome shotgun sequence.
ACCESSION   DAOSCV010000211 DAOSCV010000000
VERSION     DAOSCV010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10114_length_5161_cov_4.498629"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLY56_06595"
     CDS             complement(<1..881)
                     /locus_tag="PLY56_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ21184.1"
                     /translation="MSAVNDASQLAVNGGPKVRQQPWPGRALFGEEEKAAVVALFDEA
                     IRTGSAFGYNGPQEEAYCREFAEYLGGGYADAVNSGSSAVYVALRSLKLEPFTEVICP
                     AITDPGGIMPVAMLNCIPIVADVCPGSYNIGAEQIAACLSERTSAIIVAHIAGEPADM
                     DPILELARSRGIPVIEDCAQAHGARYKGRPVGTLGDVGAFSTMSGKHHATAAQGGVVF
                     TRNEATYWEARRCSDRGKPFGLEGVPSNVIAAPNMNLNDLSAAVGRVQLKKLDRIVAR
                     RRDVARAIHEGLKKRSRV"
     gene            complement(982..2121)
                     /locus_tag="PLY56_06600"
     CDS             complement(982..2121)
                     /locus_tag="PLY56_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21185.1"
                     /translation="MSLRIAVIGASGRGLAMARLFEQVSGCRAVAFADPFAPAREMAA
                     ERFPGAALFESCEELLDRSDVDAVLVASADHAHEANGLAVLARRKHLYLEKPMAQSVA
                     GCDALLRTWSGTNTVFMVGLELRECVLFRRVREILDAGEIGRPVMGLAIDNVSVGGDY
                     FYHNHYRKKAYTRSLLLQKGVHTIDLLNWFMGGNPCRVFASGGLDYFGRKEDPEKRCR
                     DCERARSCRYYVVPKSPAAKAADGCVWSRDIDLNDNSQLLIDYDNGSRAVYMECHFTP
                     EYTREFTLFGDEGKLTAFYNNECDFLIRVTKPGGSVKELRPAPEKGGHGGSDINLVRA
                     FFQAIEDGKCPVSFLVGARNAAAVGAAGEDSIETGLPVTIPPPPL"
     gene            complement(2250..2711)
                     /locus_tag="PLY56_06605"
     CDS             complement(2250..2711)
                     /locus_tag="PLY56_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21186.1"
                     /translation="MEIRALSLRVTEDELNGWIGRLLEGSDKVQRLQIRIGTEGLVAD
                     GEAEFMGHQVPFDATIHLHAEESCIVLRLSRFEAAGFFPVPGWFILKKLRKHQSEWLQ
                     IEDDCVTLNVERLLEERGVVLATNLSGVRYEPGALILDAQAPEPVQAAAHP"
     gene            complement(3059..3823)
                     /locus_tag="PLY56_06610"
     CDS             complement(3059..3823)
                     /locus_tag="PLY56_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ21187.1"
                     /translation="MTDSVIAAQLYTVREFLKTPADIATTLKKISDIGYKAVQVSGMG
                     PIEPAELKKIADDNGLAIVSTHIPYERMRDDFDAVVAEHKLWNCEYPGIGGLPVSYRN
                     AEGFVRFAKEASEVARRFKEHGLTFVYHNHSFELEKFGDRTGLQILYEESDPEAFTAE
                     IDTYWIQHGGGDPAWWIRSLKGRAHVVHFKDMTMKGTTQLFAEVGEGNLNWPAILDAC
                     KEAGVRWHIVEQDVCQRDPFESLAISLRNLNKMGLK"
     gene            4115..4315
                     /locus_tag="PLY56_06615"
     CDS             4115..4315
                     /locus_tag="PLY56_06615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21188.1"
                     /translation="MGAIRLANRRAFTLIELLVVIAIIAILAAILFPVFARARENARK
                     STCQSNLKQLAMGILMHVRAGS"
     gene            4296..4844
                     /locus_tag="PLY56_06620"
     CDS             4296..4844
                     /locus_tag="PLY56_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21189.1"
                     /translation="MYVQDHDEKAPPNHAAYKIVVNGNERWAEWWYLVHPYTKNYQVF
                     TCPSNSGGFSVPNPEGGPNIRVTYAKRGCYEDVIPGDGSNAFWRGDFVQIEEPAQTIC
                     LGDWGTGSGHRLCPHYHAGSTYVGYPHVNLHMDGCNYAFWDGHVKWMKYDQTLRPVNL
                     WKMSQKTAADSLPPVPAWPWPL"
BASE COUNT          935 a         1745 c         1583 g          898 t
ORIGIN      
        1 actcgcgagc gctttttcaa cccctcgtgg atcgcccgag ccacgtcccg gcgtcgggca
       61 acgatccgat ccagcttctt gagctgcacc cggccaaccg ccgcggagag gtcgttcaga
      121 ttcatgttgg gtgctgcgat cacgttgctc ggcacgccct ccaggccgaa gggtttgccg
      181 cgatccgagc accgccgcgc ctcccagtag gtcgcctcgt tgcgcgtgaa gaccacgccg
      241 ccctgggccg cggttgcatg gtgcttcccg gacatggtcg agaaggcgcc gacatcgccc
      301 agcgtgccga cgggccggcc cttatagcgc gcaccgtgcg cctgagcgca gtcctcaatg
      361 accggtatgc cgcgcgaccg ggcgagctcc aggatcgggt ccatatcggc gggctcgccg
      421 gcaatgtgcg ccacgatgat ggcgctggtg cgctcggaaa ggcaggcggc gatctgctcg
      481 gccccgatgt tgtaggagcc ggggcacacg tcggcgacaa tcgggatgca gttcagcatg
      541 gcgaccggca tgatgccgcc cggatcggtg atcgccgggc agatcacctc ggtgaacggt
      601 tccagcttca gcgagcgcag ggccacataa acggcgctgc tcccggagtt gaccgcgtca
      661 gcatagccgc ccccgaggta ctccgcgaac tcgcgacagt acgcctcctc ctggggcccg
      721 ttgtagccga acgcgcttcc ggtgcggatc gcctcatcga agagggcgac cacagcggcc
      781 ttctcctcct cgccaaaaag cgctcgtcca ggccacggct gctgccgcac ctttggcccg
      841 ccattcaccg cgagctgact ggcatcattg actgcactca tccctctctc ctccttgccg
      901 gcgcgcaggc cgcgccggcg cgcaaaactg ggagcgtagt tcccgcccgt ccgtccgggt
      961 tcctgcccgt gcggacgtgc ctcacagggg cgggggcgga atggtgaccg ggagtcccgt
     1021 ctcaatcgag tcctcgccgg cagcgcccac ggccgcggcg ttgcgcgcgc caacgaggaa
     1081 agaaaccggg cattttccat cctcaatggc ctggaagaag gcgcgtacca ggttgatatc
     1141 gctgccccca tgcccgccct tttccggcgc ggggcgcagc tccttcacgc tgccgcccgg
     1201 cttggtcacg cggatgagga agtcgcactc gttattgtag aacgccgtca gcttcccctc
     1261 gtcaccaaag agggtaaact cgcgggtgta ctccggcgtg aagtggcact ccatgtagac
     1321 cgcgcgcgag ccgttgtcat aatcgatcaa gagttgggag ttatcgttca ggtcgatatc
     1381 acggctccag acgcagccat cggcggcctt ggcggcaggg ctcttgggca cgacgtagta
     1441 acggcaagag cgtgcccgct cgcagtcacg acaccgcttc tccggatcct ccttgcgccc
     1501 aaagtaatcg agcccgcccg aggcgaagac gcggcacgga ttgcccccca tgaaccagtt
     1561 gaggaggtcg atggtgtgca cgcccttctg caggagcagg ctccgcgtgt acgccttctt
     1621 ccgatagtgg ttgtggtaga agtagtcacc gccgacggag acgttatcga tcgccaggcc
     1681 catcaccggc cggccgatct cgccggcatc aaggatctcg cgcacgcggc ggaagagaac
     1741 gcactcgcgc agctcgagtc cgaccataaa gaccgtgttc gtgcccgacc aggtgcgcag
     1801 gagggcatca cacccggcaa cgctctgcgc catcggcttc tccaggtaga ggtgcttgcg
     1861 gcgcgcgagc acggcgaggc cgttcgcctc atgagcgtgg tcggcggagg ccacgagaac
     1921 ggcatccacg tccgacctgt ccaacaactc ctcgcagctc tcgaagagcg cggccccagg
     1981 aaaacgctcg gccgccatct cgcgagcagg ggcgaaagga tctgcaaagg cgaccgcgcg
     2041 gcacccgctc acctgctcga agagccgggc catcgccagc ccccggccac tcgcgccgat
     2101 tacagcgata cgaagagaca tgatatcagc cttccaaacc ctcgcgcggg gaagggagcc
     2161 ctgcccaccc cggggccagg taacacgcga gcgctcccca cgtgccagcg ctttccaccc
     2221 tgcgcgccag ccgtcaccga tgggtctcgc tacggatgag ccgcagcttg caccggctcg
     2281 ggcgcctggg catccagaat gagcgcaccc ggctcgtagc gcacgccgct cagattcgtg
     2341 gcaagcacga caccccgctc ctccagaagg cgttccacgt tcagcgtcac gcagtcgtcc
     2401 tcgatctgga gccactcgga ttggtgcttg cggagcttct tcaggatgaa ccaacctggc
     2461 accggaaaga accccgccgc ctcgaagcgt gacaggcgca ggacaatgca gctctcctcc
     2521 gcgtggaggt ggatcgtggc gtcgaaggga acctggtggc ccatgaactc ggcctcccca
     2581 tcggcaacca ggccctcggt gccaatccgg atctgcaacc gctggacttt atcgctcccc
     2641 tcgagcaagc gaccaatcca tccgttcaac tcatcctcgg ttacgcgcaa gctcagggcg
     2701 cggatctcca tcggctacct tcccgatcgt tctccttgaa ccggaacgcc gcgctccaag
     2761 gagcgccacg ctgcttccat caacttcatc acgtgccggc cacgcagcgg gccatgggga
     2821 accatccggc tgtggcgcaa ctcgcgggac cgcagagtgc ccggtcgctc cctctaacca
     2881 ggattgcaca ccgggggtcc aaaatcctgt ttgaaaggtt gcttctgata tcatccgtca
     2941 caggcggagg gtttcttaga agtacgcggg aagcgagggg agatggccaa gggcggcccg
     3001 gcgatgcgct ggcgcgcgtc cccaggccgt cctcacgttc agtgcaagct ggcgcggcct
     3061 acttgaggcc catcttgttc aggttgcgca ggctgatcgc caggctctcg aagggatccc
     3121 gctggcagac atcctgctcg acgatgtgcc agcgcacgcc cgcttccttg cacgcatcga
     3181 ggatggcggg ccagttcagg ttgccctctc ccacctcggc gaagagctgc gttgtcccct
     3241 tcatcgtcat atccttgaag tggacaacgt gcgcgcgccc tttcaggctg cggatccacc
     3301 atgccgggtc gccaccgccg tgctgaatcc agtaggtatc gatctcggcc gtgaacgcct
     3361 ccgggtcgct ctcctcgtag aggatctgca ggccggtgcg atcgccgaac ttctccaact
     3421 cgaagctgtg gttgtgatag acgaaggtga ggccgtgctc cttgaagcgg cgggccacct
     3481 ccgacgcctc cttcgcgaag cgcacgaagc cctcggcatt gcgataggag accggcaggc
     3541 cgccgatgcc cgggtattcg cagttccaca gtttgtgctc ggccaccacg gcgtcgaagt
     3601 catcgcgcat ccgctcgtag gggatgtgcg tcgagacgat cgccaggcca ttgtcatcgg
     3661 cgatcttctt cagctccgcc ggctcgatcg gccccatccc ggaaacctgc accgccttgt
     3721 aaccgatatc gctgatcttc ttcagcgtgg tggcgatatc ggccggcgtc ttgaggaatt
     3781 cgcgcacggt gtagagttgc gcggcaatca cggaatcggt catcttcgca tccttccttt
     3841 gccaacccaa caggggcggg ctgacccagc gtccctacac cacacattcg gccctcaccc
     3901 gccaacgcct tctccgattc ggcgcattgc gagttgcgcc gatgcccatt tgatggtata
     3961 ctaggccatg cgtgtcctaa ccgctcgctc tcacatctgt cgggcggcgg gcaggcggca
     4021 acgccgcgca ctagtcacag gcaaggcatc tatcgccgcc ccctcaaacg gtgcaaaccc
     4081 tgttgttcaa ggtgaccagc aaaggagggt tccgatgggc gccatcaggc ttgccaacag
     4141 aagggcgttt accttgatcg agctgctagt cgtgatagcc atcatcgcga ttctcgcagc
     4201 catcctcttt ccggttttcg cgcgcgcaag ggagaacgcg cggaaatcga cctgccagag
     4261 caacctgaag cagctagcta tgggcattct catgcatgta cgtgcaggat catgatgaga
     4321 aggccccgcc caaccacgcc gcctacaaaa tcgtcgtcaa cggcaacgaa cgctgggccg
     4381 agtggtggta cctggtgcat ccctacacga agaactacca ggtgttcacc tgcccgtcca
     4441 atagcggcgg cttctcggtg cccaacccgg aaggcggacc gaacatccgc gtcacctatg
     4501 ccaagcgcgg ctgctatgag gacgtcatcc ccggcgatgg cagcaatgcg ttttggagag
     4561 gcgatttcgt tcagatcgag gagccagccc agacgatctg cctcggcgac tggggcacgg
     4621 gtagcggcca ccgtctctgc ccgcactacc atgccggaag cacgtacgtc ggctaccccc
     4681 acgtgaacct gcacatggat gggtgcaact acgcgttctg ggacggccac gtgaagtgga
     4741 tgaagtatga tcaaacgctg cgcccggtga acctatggaa gatgtcgcag aaaaccgcgg
     4801 ccgatagcct cccgcctgta cccgcatggc cctggcctct ctaagcggcg cgactcagat
     4861 tttggcgcgg gacggcgaga gaggctgcgc gtcccgcgcc cccgtaaacc ccacccggga
     4921 ggcagccgcg cctgggcgcg ggcatcctgc cggcctgccc gcttctctgg gattccgata
     4981 taaggaatcc gaagcctcgg aggtagcgcg caacccgtcg cgcggcgata aatcaccgcg
     5041 ctgcttgcga gcgaagccct gccgggctgg gttggggata ctcccagatg tctcaaaacg
     5101 gcggtggctg ggaggggaag ccccgagggg gctttgccgg aaagcagcgc ggagcttcga
     5161 g
//
LOCUS       DAOSCV010000212         5160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10122_length_5160_cov_3.328501, whole genome shotgun sequence.
ACCESSION   DAOSCV010000212 DAOSCV010000000
VERSION     DAOSCV010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10122_length_5160_cov_3.328501"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLY56_06625"
     CDS             <1..619
                     /locus_tag="PLY56_06625"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ21190.1"
                     /translation="EEVLPRFRGAIQQIPPMYSAIHHQGQRLYELARRGKEVERRPRD
                     VVIHQLELLSFEPGTTARARLRVVCSKGTYIRALCADLGEALGIPAHMCALTRTRVGP
                     FRIEDAVSLEELAEAASGPQGLAGLFQTGLLRPPDAAVAHLPCEQVKGDALVRVRHGN
                     AIPVRTPAEEDRPVRIENNEGELVGIGHYERVADEWRLQPDKIME"
     gene            879..1628
                     /locus_tag="PLY56_06630"
     CDS             879..1628
                     /locus_tag="PLY56_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21191.1"
                     /translation="MNSALRHRIALGICLTACGTFLLAGSQSGHAAPAKRTTAKPAAK
                     AAAKPAAEVTVKSKVSSFTNGVLLLTNGKKFRLDPVQTAVTTRRGKTPPTQWQATISP
                     GDEVTVVYDSKKAITRSVKIEPPYQLPNGEYEVLGQTTYLYSNPSNPAAESKEVPLLW
                     VRSSDPSKKQRYVVELIDTTALVGEGIEAGTAGSQAIKQMLQPKSIIQLQMFTSVFPT
                     VDKYGDPESPTAVNGVVKLIKVTQAPAATEN"
     gene            complement(1734..2726)
                     /locus_tag="PLY56_06635"
     CDS             complement(1734..2726)
                     /locus_tag="PLY56_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ21192.1"
                     /translation="MPEDHALRDVLVVDWPESAAAELGHLLEEAGFRPCPAGSGAEAG
                     ALVSTHTYAAAVISSKLPDMPGGACIRELQRRDRDLPCVVVATAPTVESVIAVANLCV
                     FGYVVRPVRPREFLDLVTRAVEHRRLEAELRAERLRTAQLLGVLQTIETIQDRINNPL
                     QVILGYAELLSSSHDEEDAETHQFAHRIMEAGGAIADVIAKLRRVRRPMTQAWSFGET
                     LDLDQAGPPPNDRRQHPRFISVMLVRFRGRAGWRWHYVDDISRGGLAIHITGATEIPE
                     CVELELADPESGQTYQCQAEKVWVDPNQARAGLRFTMLDPRLAERLRPEPGGPS"
     gene            2929..3972
                     /locus_tag="PLY56_06640"
     CDS             2929..3972
                     /locus_tag="PLY56_06640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ21193.1"
                     /translation="MPGKGRRLLVTGAAGFMGSHLVDALIAEGHTVFGVDDMSGGYWR
                     NIHPQSEFTVLDLRNREATDAYIRKVQPEIIFHMAADATEGRSQFTPLSSTERNYLAY
                     LNVLVPAIREGMRKMVLISSMSVCGAQSPPFHEDMPRQPEDIYAIAKAAMERATEILA
                     EVHGFQYTIIRPHNVYGERQNIADPYRNVIGIFINRLLAGKPFFIYGDGEQKRAFSHI
                     DDFTPYTVRAGFDDVANGQIFNIGPREEYTINEVARLVLKHFGYDPDNPPPEVKPIYH
                     PPRPKEVKEAYCTLDKAQRLLGYRTTVSLDEGIRRMVAWAKELGPQPMRYLKDGLELI
                     TENTPATWVKQMM"
     gene            complement(4064..4279)
                     /locus_tag="PLY56_06645"
     CDS             complement(4064..4279)
                     /locus_tag="PLY56_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21194.1"
                     /translation="MTGKDGKRPNWLIWDGRGTRVCELLNFWRETGYWWEGEHEKDFY
                     RVQCDRRQYDIYRDRVTGEWFLYRIHE"
     gene            4588..4908
                     /locus_tag="PLY56_06650"
     CDS             4588..4908
                     /locus_tag="PLY56_06650"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21195.1"
                     /translation="MKTHQHSRQKGFTLIELLVVIAIIAILAAILFPVFARARENARK
                     ATCQSNINQLNKGILMYAQDYDERLPTRYDGKAQWKDQIMPYLKNTDSLRCPSTKQYS
                     IGWA"
     gene            4960..>5160
                     /locus_tag="PLY56_06655"
     CDS             4960..>5160
                     /locus_tag="PLY56_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21196.1"
                     /translation="MILCDAGMVNTNTGGKAYDWHFNIPSLFGNPPLPPADEVNGIPL
                     PSDTAYQGRPCPLHMDGCNVGFV"
BASE COUNT         1018 a         1669 c         1573 g          900 t
ORIGIN      
        1 cgaggaggtg ttgccccgct tccgaggcgc cattcagcag attcccccga tgtactccgc
       61 gatccaccac cagggacagc ggctctatga actggcgcgc cgcggcaaag aggtggaacg
      121 ccgcccccga gacgtggtga tccatcagct cgagttgctg tcgttcgagc cgggaacgac
      181 ggcgcgcgct cggctccggg tcgtctgctc caagggaacc tacatccgcg cgctctgcgc
      241 cgatctgggc gaggcacttg gcatccccgc gcacatgtgc gcgctcacgc gaacccgcgt
      301 aggccccttc cggattgagg acgccgtgag cctggaggag ctggcagagg ccgcttccgg
      361 gccacagggt ctggccggcc ttttccagac cggcttactg cgcccgccgg acgcggctgt
      421 ggcgcacctg ccgtgcgagc aagtgaaggg agacgccctg gtgcgcgtgc gtcatggaaa
      481 cgccattccc gtgcgcactc ccgcggagga ggatcgaccg gttcgcattg agaataacga
      541 aggcgagttg gttgggatcg ggcactacga gcgtgttgcc gacgagtggc gcctgcaacc
      601 ggataagatc atggagtagc cctccccttg ggagggcgcc agcccctgca gagggaactg
      661 gacactgccg ataatagcac taggaaaccg gagttggccc gcgcccgccc gtgcatgacg
      721 gcgtgcccgt gccagtatct cgcggcaagg atgcttgcgg gccagcgcag gttctgccca
      781 caagcccccg cacttgtcgc gccgtgaagc tgaacgcaaa gcgcatctcc ccgggggcca
      841 tgccctcggg caccatcgcc tgcttggagg agaagttgat gaattctgcc ctgcgtcatc
      901 ggatagccct tggcatttgc ctgactgcct gcggcacgtt tctgcttgcc ggatcccaat
      961 cgggccacgc cgcccctgcc aagcgcacca ccgcgaagcc ggccgcgaag gcagccgcaa
     1021 agccggccgc cgaggtaacg gtcaagagca aggtctcttc gttcacgaac ggcgtccttc
     1081 tgctcaccaa cgggaagaag ttccggctgg atccggtgca gacagccgtg acgacgcgcc
     1141 gtggcaagac gccgcccacg caatggcaag cgaccatctc gccgggggac gaggttaccg
     1201 tcgtctacga ctcgaagaag gctatcactc gatctgtgaa gatcgagccc ccttaccagc
     1261 tccccaacgg ggagtatgag gtgctcggac agacgaccta cctctacagc aacccgagca
     1321 atcccgcggc agagagcaaa gaagtgcccc tcctgtgggt ccggtcttcc gacccgagca
     1381 agaagcagcg ctatgtcgtg gagctgatcg atacaaccgc gctggtcggc gaaggcatcg
     1441 aagcgggcac cgccggcagc caggcaatca agcagatgct ccagcccaag agcatcatcc
     1501 agctccagat gttcacctcg gtgttcccca cggtggataa gtacggcgat cccgagagcc
     1561 cgaccgcggt gaacggcgtc gtgaagctga tcaaagtcac gcaggcgcca gcggccaccg
     1621 agaactgagg agtctacctg ccgtcccgtt gtgacggtgg cgtcttattg ggataagggg
     1681 aagggcgagc ccacccatgc ttgcccctcc ccttttgctg tttccacgca gtctcaggat
     1741 ggcccgcctg gctcgggtcg gaggcgctcg gcgaggcgcg ggtccagcat ggtaaaacgg
     1801 aggcccgctc gggcctgatt gggatccacc cacacctttt ccgcctggca ctgataggtt
     1861 tgtccgctct ccggatccgc cagttccagc tcgacgcatt cggggatctc cgtggcaccg
     1921 gtgatgtgga tggccaggcc accacggctg atgtcatcca catagtgcca ccgccagccc
     1981 gctcgcccac gaaagcgcac cagcatcacc gaaatgaaac ggggatgctg acgccggtcg
     2041 ttggggggcg ggccggcctg gtcgagatcg agcgtctctc cgaaggacca tgcctgggtc
     2101 ataggccggc gcacgcggcg caacttggca atgacatccg cgatagcgcc gcccgcttcc
     2161 ataattcgat gagcgaactg atgggtctcc gcgtcctcct catcatggga gctgctgaga
     2221 agctcggcat agccaaggat aacctgcagc ggattgttga tacggtcctg gatcgtctcg
     2281 atggtctgca gcaccccgag gagctgggcg gtccggaggc gctcggctcg cagttctgcc
     2341 tcgaggcggc gatgctccac agcgcgcgta accagatcga ggaactcacg cgggcgaact
     2401 ggacggacca catagccgaa gacacagagg ttggcaaccg cgatgacgct ctccacggtg
     2461 ggagcggtgg cgaccaccac gcagggaagg tcgcggtcgc gacgttgcaa ctcgcggata
     2521 caagccccgc caggcatatc cggaagcttg ctggagatga ccgcagcggc gtaggtgtgg
     2581 gtggaaacaa gcgccccggc ttccgcgcca gatcccgccg gacacggcct gaatccggct
     2641 tcttcgagta gatgacccaa ctcggccgcg gcgctctctg gccaatcgac gacgaggaca
     2701 tccctcaggg catgatcttc aggcattcct gcatcctaat gctgcccggc ggtggcccac
     2761 ccgccgcagc cgcgcttttt actcttttgt tccgtatgat ggcggtactt cctcctctca
     2821 ggattgcccg actgcaaagg agatggtata atgcggtatc ggcgtccggc cccgcctgac
     2881 caggcggctg ccggtgcacg cgacaccata tggagatggg gagaagcgat gccaggaaag
     2941 ggcaggagac tgcttgtcac cggagccgca ggcttcatgg gctcacacct cgtagatgcc
     3001 ctgattgccg aagggcatac ggttttcggc gtggatgaca tgtcgggcgg atactggcgg
     3061 aatattcacc cgcagagcga gttcaccgtg ctggacctgc gcaaccgcga ggccacggac
     3121 gcctatatcc gcaaggtgca gcccgaaatc atcttccaca tggcggcaga tgccacggag
     3181 gggcgcagcc agttcactcc gttgagcagc accgagcgaa actaccttgc ctatctcaac
     3241 gtgctggtgc cggcgatccg tgagggaatg cgcaagatgg tgctcatctc ctcgatgagc
     3301 gtttgcggcg cgcagtctcc tcccttccac gaggatatgc cgcgccagcc cgaggatatc
     3361 tacgcgatcg ccaaggccgc gatggaacgc gcgaccgaga tcctcgctga ggtacacggg
     3421 ttccagtaca ccatcatccg gccgcataat gtgtacggag agcgccagaa cattgcggat
     3481 ccttaccgca acgtcatcgg catcttcatc aaccgcctgc ttgcaggcaa gcccttcttc
     3541 atctacggcg atggcgagca gaagcgcgcc ttctctcaca tcgacgattt cactccctac
     3601 acggtgcgcg ccgggttcga tgatgtcgcc aacgggcaga tcttcaacat cggcccgcgg
     3661 gaagagtaca ccatcaacga ggtagcacgc ctggtgctga agcacttcgg ctatgacccg
     3721 gacaacccac cgcccgaggt gaagccgatc taccacccgc cccgccccaa ggaggtgaaa
     3781 gaggcctact gcaccctgga caaggcgcag cgccttcttg gctatcggac gacggtgtcg
     3841 cttgatgagg ggattcggcg catggtggcc tgggccaagg agctgggccc gcaaccgatg
     3901 cgctacctga aggatggcct ggagctgatc actgaaaata cgccggccac ctgggtaaag
     3961 cagatgatgt agcaggggcg tatggtcccc tctcctgtct aagcggcggg agcgctcttc
     4021 gcgcgctccc gccgcttaga caggcaatcc tgagacgcga cattcactcg tggatccggt
     4081 agaggaacca ctcgccggtg acacggtcgc gatagatgtc gtactgtctc cggtcgcact
     4141 gcacgcggta gaaatccttt tcgtgctcgc cctcccacca gtagccagtc tcgcgccaga
     4201 agttgaggag ctcgcataca cgcgtgcccc ggccatccca gatcagccag ttgggccgct
     4261 ttccgtcttt tcctgtcaag gcgcagagcg ccgtccgcga ttgatttgcc gccgtttgac
     4321 acccgcctac cgctggaacg ggaatggttc gtccgtccgg ggaggattcg cgcgtatgag
     4381 ctccgaagca gttcctcagc acggcccgat catctcatga atcatcgacc gtccgcgagg
     4441 aggataggtc gaatgaacag gtagctggca gcacaagcga gtgcgtgatc ctgccgcctg
     4501 gccatctgat ggttcccgcc tgcgcactcg cgcactctac ggagtgacgg accgccccgc
     4561 agcagtcgga gcagaaaggg attagagatg aaaacgcacc agcattcgcg acagaaaggc
     4621 tttaccctca tcgagctgct cgttgtgatc gccataatcg ccatcctggc ggcgatcctt
     4681 ttcccggtct tcgcccgtgc gcgtgaaaat gcgcgaaagg ccacctgcca gtcgaacatc
     4741 aaccagctca acaagggcat tctgatgtac gctcaggact atgatgagcg cctgccaacc
     4801 cgctacgatg gcaaagctca gtggaaagat cagatcatgc cctacctcaa gaacaccgat
     4861 tccctgagat gccccagcac caagcagtac tccatcggct gggcctaggg ctacctcgat
     4921 agggtcacgc tggcccagat ccagtcgccc gctgaaaccg tgatcctctg cgacgcggga
     4981 atggtgaaca ccaacaccgg cggcaaggcc tacgactggc acttcaacat cccaagcctc
     5041 ttcggcaacc cgcccttgcc accggcggat gaagttaatg gcattcctct tcccagcgat
     5101 accgcctacc agggtcggcc ctgtcccctg cacatggatg gctgcaacgt cggcttcgtg
//
LOCUS       DAOSCV010000213         5157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10127_length_5157_cov_3.182869, whole genome shotgun sequence.
ACCESSION   DAOSCV010000213 DAOSCV010000000
VERSION     DAOSCV010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10127_length_5157_cov_3.182869"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..4489
                     /locus_tag="PLY56_06660"
     CDS             137..4489
                     /locus_tag="PLY56_06660"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ21197.1"
                     /translation="MARFILRATFLVAALWLPALVAAAPAAPHVNLLANPSFEEAAKD
                     DPTRAAGWASYQCGYTRTRDRSYAPEINGPWSVRIAGTGKDDEKGFGGLNTHVTTDLP
                     AHGKFVAYNSIYIDSYTQGAIYGAYITATYTDGTQKTFSFLLSDEQIRSNLKRWKTYR
                     LVFSTDPEKKLRDITHWCLVWRKGETKFIGTVYFDDLELRLLPEGEAMGASVPFTAVS
                     RTATPPALDGRADDACWKESPQFSPFVLASGLELASQQTAVRATYDDRHLYLFLECAE
                     SVLDPAVQKREAFKAQYTEHDSAVYKDDAVELFLMPPGGEAYYHLAINSLGTVYDARC
                     RSDSAVDPGWNSGAVAKGQVGERAWSLEVAIPWQAMGARAPQAGEAWRVNICRAEQAN
                     KENSCWSPTGGAFHTPASFGVASFGASPVAVDVIDLGRRGKGTNELRLSLTNPSAQPV
                     AVTVNAFIDGTGRPVEAGRQVVRLGPGATERVKVEYVSPGGGGVLRYEVAEAGRVLYT
                     SAGFPLRFDTVFMARVTVLVANHTRPITEFHVVQGEWLALPLVVTPSVSPEAFREAQA
                     ILEVPRFLRLVNPLAGARRVPAPLRVTEARIERDGKPYRRYTLELGAAHVTFPEAAAQ
                     REFVLNPLLFCADLTGSRPEEVATYPLGFDIRVNGQSSVAGEVPITLLSPLPRRSPSN
                     LVVCNWPCGMAFQVPFFYRLSELEQRTIAESWLRAGFNFSMHTGILREVGKGHPIKSG
                     YMLPGNLNDISASLPGIAEYLAAHPQYRTVCLNGKVLDRVVSPAHLLEPDCPLRPLVR
                     ELAGKLAKQYPVLSWDYEVPVLLPQSIGFDAANLAAFRRFAKIPEAAPLTPEIVARDY
                     RELWVDFRCRQNAEIAGLLQEGIKQANPECLFFVYSGYQGPNTRQTYGVNWEYMRPHI
                     DQGWCGYGRPVSLIQETRRALADRPLVGGELAWFGDGTPYNQDEAEPHLFRRLTDSAG
                     GFMVYYDWFLDGRFTRAIARAAAVAADFEAFFLQGKRDDSLAAVDAGDAANVAVYALG
                     DERLLFLFNHTAKPQPFRITMAGLAPGVAGIDYWTRKPVSLRPALETTVPAYTVQVIY
                     ACRQNTGAVPTTPRLLEPSGEIVTDYRPLLRWENEGSARCTYTIELSREKDFPAASTR
                     RIEGVPGTSWVVSEALDETARIYWRVRAVDALTGKASAYSRVGEFTLGTLGVEVAPAV
                     FSPNGDGKYDTASLRAELRSEAPWKVAVASASGQVVREIAGQGDHVEITWDGKDAGGK
                     VVPDGRYLLRLVVKGRDVASERVEVNSRFGVPNPRLEQWCEWQAVALEGGETLQDYLV
                     APEGLPYSLKLTGRDTEAKAYWSNYRTGTEIPITPGKEYVFTGLVKGDLAPDGVAEIR
                     LHFFTKENRWAPIPGLSAEWDGVTAELKGKKEWTRLTVSCRAPENAAKAVLFFRLTGK
                     GSAWFGAAEFGEKK"
     gene            4623..>5157
                     /locus_tag="PLY56_06665"
     CDS             4623..>5157
                     /locus_tag="PLY56_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21198.1"
                     /translation="MKRRSLGFLRGRVEEGKVCLTAYKTQRYCGEEYAAEFSRLRVYR
                     KEVEFLFNTDYAEFFDGLSPSPSDLLFEGPLEAGNGRKFTYIDRTAQVGRTYAYFLGS
                     ASGDIPLTGPVPVRVRDQEVWWPYARVVDRLRRMEQAYPGLARVEEIGRTARGNALHG
                     VLVGRGERTIGLVGVIHA"
BASE COUNT          836 a         1659 c         1797 g          865 t
ORIGIN      
        1 tatcgcacca agtaggggct tggcgaaagg actcggcagg cgcttcgcag ggcgcgaagc
       61 gcctgcccca ttgccggcgc tctccctctt cccctgactt gttgagagcg acccgctcag
      121 agaggagctg ctcctgatgg ctcgattcat actccgcgca acattccttg tcgcggctct
      181 atggctgccg gcgctcgtgg ccgccgcgcc ggctgctcca cacgtgaacc tgctcgcaaa
      241 cccctccttt gaagaagcag ccaaggacga ccccacccgg gccgcgggct gggcctctta
      301 ccagtgtggc tacacccgga cgcgcgaccg gagttacgct ccggagatca acggtccatg
      361 gagcgtgcgc atcgctggta cgggcaagga cgacgagaag ggctttgggg gattgaatac
      421 ccatgtgacc accgacctcc ccgcgcacgg gaagttcgtc gcctacaaca gcatctatat
      481 cgattcctac acccagggcg cgatctacgg ggcctacatc accgccacct acaccgacgg
      541 cacccaaaag actttcagct ttctcctctc cgacgagcag atccgctcga acctgaagcg
      601 atggaagacc taccgcctgg tcttctccac cgacccggag aagaagctga gggacatcac
      661 ccattggtgc ctggtgtgga ggaagggcga gacgaagttc atcggcacgg tctacttcga
      721 tgatctggag ctgcgcctgc ttccggaggg cgaagcgatg ggcgccagcg tgcccttcac
      781 ggcggtgagt cgcacggcca ctccccccgc cctcgatggc cgggccgatg acgcgtgctg
      841 gaaggagagc ccgcaattca gccctttcgt gctggcgagc ggcctcgaac tcgcgtcgca
      901 gcagacagcc gtgcgcgcga cgtatgatga ccgccacctc tacctctttc tggagtgcgc
      961 ggagagcgtt ctagaccccg cggtgcagaa acgggaggca ttcaaagcgc agtacacgga
     1021 gcacgactcg gctgtgtaca aggatgacgc ggtcgagctt tttctgatgc cgcccggcgg
     1081 cgaggcatat taccacctcg cgattaacag cctgggcacc gtgtacgacg cgcgctgccg
     1141 gtccgattcg gctgttgacc cgggctggaa ctcgggagcg gtagcaaagg ggcaggtagg
     1201 cgagcgcgcc tggagcctgg aagtggcgat cccctggcag gcgatgggcg cgcgggcccc
     1261 ccaggcggga gaggcctggc gggtcaacat ctgccgcgcg gagcaggcca acaaggagaa
     1321 ctcctgctgg tcacccacgg gcggcgcctt ccacacgccg gcgagcttcg gagtcgccag
     1381 ttttggggcc tcgccggtgg ccgtggatgt gattgacctg ggccggcgcg gcaagggaac
     1441 gaatgagctc cgcctgagcc tgacgaaccc ctcggcgcag ccggtcgccg tgacggtgaa
     1501 cgccttcatt gacggcaccg ggaggccggt ggaggccggg cggcaggtcg ttcgcctggg
     1561 tccgggcgcc accgagaggg tgaaggtgga gtacgtgtcg ccgggcggcg ggggcgtgct
     1621 ccgctacgag gtggcggagg ccgggcgcgt gctctacacc tctgccgggt tccccctccg
     1681 gttcgacacg gtcttcatgg ctcgggtgac ggtactggta gccaaccaca cgcggccgat
     1741 cacggagttc cacgtggtcc agggagagtg gcttgccctc ccgctggtgg tcaccccgag
     1801 cgtcagtccg gaggcgtttc gcgaggcgca ggcgatcctg gaagtgcccc ggtttctgcg
     1861 cctggtgaac ccgctcgcgg gtgcgcgccg ggtgcccgcg cccttgcgcg tgaccgaggc
     1921 ccgaatcgag cgcgatggga agccataccg gcgctacacc ctggagctcg gcgccgcgca
     1981 cgtgactttc cccgaagcgg cggcgcagcg cgagttcgtc ctcaatccgc tcctcttctg
     2041 cgcggatctc accggatcgc ggcccgagga agtggcgacc taccccctcg gcttcgatat
     2101 ccgggtgaat ggccagagct cggttgccgg cgaggtgcct atcacgcttc tctcgccgct
     2161 gccgagacgc tcgccgtcca acctagtggt gtgcaactgg ccgtgcggca tggcctttca
     2221 ggttccgttc ttctaccggc tgagcgagct cgagcagcgc acgattgccg agtcgtggct
     2281 gcgcgcgggg ttcaacttct cgatgcacac cgggatcctg cgtgaggtgg gcaaaggcca
     2341 tccgatcaag agcggctaca tgctgccggg caacctcaac gacatcagcg ccagcctgcc
     2401 tgggatcgcc gaatacctgg cggcgcatcc ccagtaccgc accgtgtgcc tgaacgggaa
     2461 ggtgctggac cgcgtggtct cgccggcgca cctgctggag ccggactgcc ctctccggcc
     2521 gctcgtgcga gagctcgccg gcaagctggc aaagcagtac cccgtgctga gctgggacta
     2581 cgaagtgccc gtgctgctcc cgcagtcaat cggtttcgat gccgccaacc tggcggcgtt
     2641 ccggcgcttt gccaagatcc cggaggcggc gccactgacg cctgagatcg tggcgcgcga
     2701 ctaccgcgag ctctgggtcg atttccgctg ccgccagaac gctgagatcg ccggcctgtt
     2761 gcaggagggg atcaagcagg ccaacccgga gtgcctcttc tttgtctaca gcggctacca
     2821 ggggccgaac acgcgccaga cgtacggcgt caactgggag tacatgcgcc cgcacatcga
     2881 ccaggggtgg tgcggctatg gccggcctgt gtctctgatt caggagacgc gacgcgcgct
     2941 ggcggaccgg ccccttgtgg gaggcgagct ggcgtggttt ggcgatggca ctccctataa
     3001 ccaggatgag gccgagccgc atctcttccg gaggctgacc gattccgccg gcgggttcat
     3061 ggtctactac gactggttcc tggatggccg gttcacccgg gcgatcgcgc gcgcggccgc
     3121 ggtggccgcc gatttcgagg cgttcttcct ccaggggaag cgcgatgatt cgcttgcggc
     3181 ggtggacgcg ggcgatgccg ccaacgtcgc cgtctatgcc ctcggcgacg agcgcctcct
     3241 cttcctcttc aaccacacgg caaagccaca gcccttccgg atcacgatgg ccggcctggc
     3301 gccgggcgtc gccgggatcg actattggac gcgcaagccg gtctcgctgc ggcccgcgct
     3361 ggagacgacg gtgcccgcgt acaccgtaca ggtgatctac gcgtgccggc aaaacacggg
     3421 ggcggtgccc accacgccgc gcctgctgga gccttccggc gagatcgtga ccgattaccg
     3481 cccgctgttg cgctgggaga acgaggggag tgcgcgctgc acctacacga tcgaactctc
     3541 gcgggagaag gacttcccgg cggcgagcac gcgccggatc gagggcgtgc cgggcacgtc
     3601 atgggtggtc tctgaggctc tggacgagac cgcgcgcatc tactggcggg tgcgcgccgt
     3661 ggatgccctc accggcaagg cgagcgctta ctcccgcgtg ggcgagttca ctctcggcac
     3721 gctaggcgtg gaggtggcgc cggccgtctt ctcgccgaat ggcgacggga agtatgacac
     3781 ggcctccctg cgggcggagc tgcggagcga ggcgccgtgg aaggtcgccg tggcgagcgc
     3841 gtccggccag gtcgtccgcg agattgccgg gcaaggcgac cacgtcgaga tcacctggga
     3901 cgggaaggat gctgggggaa aggtggtgcc cgacggccgc tatctgctcc gtctcgtggt
     3961 gaaggggaga gatgtcgcct cggagcgcgt ggaggtcaac tcgcggttcg gcgtgcccaa
     4021 cccgcgcctg gagcagtggt gcgagtggca ggcggtggcg ctagagggcg gtgaaaccct
     4081 ccaggactac ctggtagcgc ccgagggcct gccatactcc ctcaagctga ccgggcgcga
     4141 tactgaggcg aaggcctact ggtccaacta ccgcacgggc accgagattc cgatcacgcc
     4201 cggaaaggaa tacgtcttca ccgggctggt gaaaggcgat ctggctcctg acggggtggc
     4261 ggagatccgc ctccacttct tcacgaaaga gaaccggtgg gcgcccattc cgggcctgtc
     4321 cgccgagtgg gatggcgtca cggccgagct gaaagggaag aaggagtgga cgcggctcac
     4381 cgtctcgtgt cgcgccccgg agaacgcggc gaaggcggtg ctcttcttcc gcctcacagg
     4441 gaaggggagc gcctggttcg gcgcggccga gttcggcgag aagaagtgag ccatcccacg
     4501 gcgcaggcgc gcgggcttct gcccctcgcg ctgcctgtgg gagttttgca tcttacgggg
     4561 tgcaggcatc tcgcctgggc gagatgcctg caccctggca tttcagtgtg atgggagcgg
     4621 cagtgaagcg acggtcactc ggatttcttc gcgggcgcgt tgaggaagga aaggtctgcc
     4681 ttaccgccta caagacgcaa cggtattgtg gagaggagta cgcggcggag ttctcgcgcc
     4741 tccgggtcta ccggaaggaa gtggagtttc tcttcaacac cgactacgct gagttcttcg
     4801 acgggctctc tccctcgccg tccgatctcc tcttcgaggg cccgctggag gcgggcaacg
     4861 ggcgcaagtt cacctacatc gaccgcaccg cgcaggtggg gaggacctac gcctactttc
     4921 tcggatccgc cagcggggat attccgctga caggccccgt gccggtgagg gtgcgtgacc
     4981 aggaggtgtg gtggccctat gcgcgggttg tggaccgcct gcgccggatg gagcaggcat
     5041 atccgggact ggcgcgcgtg gaggagatcg ggcggacggc acgcgggaac gccctgcacg
     5101 gcgtcctcgt tggccgcggt gagcggacga ttggtctggt gggcgtgatc cacgccg
//
LOCUS       DAOSCV010000214         5155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10132_length_5155_cov_3.648039, whole genome shotgun sequence.
ACCESSION   DAOSCV010000214 DAOSCV010000000
VERSION     DAOSCV010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_5155_cov_3.648039"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..>428)
                     /locus_tag="PLY56_06670"
     CDS             complement(167..>428)
                     /locus_tag="PLY56_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21199.1"
                     /translation="GATVTVPSEFIVMPATGDAIRSGRLEEVADGIDAVGFSLHDALV
                     RLVVEEKVSLAEAEGHAIDRRRFREEVSQRLKRVSGPIGAGP"
     assembly_gap    429..438
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<439..1546)
                     /locus_tag="PLY56_06675"
     CDS             complement(<439..1546)
                     /locus_tag="PLY56_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ21200.1"
                     /translation="MAQIEALFEFLIHQGGYELFIPSDDNCQLTDATGTHPLNQVMSA
                     REVAALIHEILPVSQRDAWSTGQRVELVQRTGAGEFRVTAEQSEEASWIRVARTPRNY
                     IEHLAAMAREAGAEELLLAEGAPAFQRREGVTERLGNAPAMPDGMLAAFLRQEAPTPG
                     EDGSPRRFLTSLACAGRARVTSLWGEAGPAAVFRLLPEEVSLPDDLPASFRELIHRRS
                     GLVLVAAQPGQGLTTLLTALLCMVAAARVAHIVTIEDLVEIPVPAENALLTRCVLGTD
                     GRRGEAILAAMTLMPEVLFVDEIPDAATLWACLDAARAGILVIAGCHGVTVQAALGRL
                     LALVGPETRAEVHERLGEALLGAVAQTLCRSKDGK"
     gene            complement(1740..3101)
                     /locus_tag="PLY56_06680"
     CDS             complement(1740..3101)
                     /locus_tag="PLY56_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21201.1"
                     /translation="MQLPIREAYCPGHFGNSYEAMWPREMAAYLAEIRHWGFNRFGDW
                     MTCTDVCNPYATDAYWNLPLEMYSRKKAAFRAAQELGMANDLILTPNHVFLDQLRPDL
                     LATKAPKIFGQLLCPSKPEARTIMLENADRQFRDLAESGVRIDSFTAFAYDYGGCACA
                     DCAPWILTWARLVRDIHAVAQRYYPEIEPWLCAWWWTEEEHVLFNDWAAREAPGWAKA
                     IIFHIPYGRTRFADLPVPEGCRRLAFIHIGYGDVPGNGDIYAKHGAVVASQRLSATLD
                     EIATMGAEGFQAYSEGQFDDINKALVAGLSSGQFDDASAVLRAYSARYFGASPAQAER
                     WASWLAAWGDRAAVDLPAATQEFEALSREATPGWRLEHLRSKLTLERLDREIGPEGEW
                     DARRLALADAFWAEQERLQRDAYRLGPVRHIFAPKFSPPKWYESWRAVSGAAAAQAMP
                     EEA"
     gene            complement(3252..3668)
                     /locus_tag="PLY56_06685"
     CDS             complement(3252..3668)
                     /locus_tag="PLY56_06685"
                     /inference="COORDINATES: protein motif:HMM:NF015720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HOJ21202.1"
                     /translation="MIRALTHRTVDYVVGIVLVLAPFLFGFWGVASATWVAILTGLVL
                     IVYSLFTQYAPGMDRNISLSTHLTLDIILGIFLAISPWLFGFAGATWNVWVPHLLVGL
                     LFIATSLLTTRVAAGQTSMAGQLGGEAPPEERRRRS"
     gene            complement(3890..4315)
                     /gene="mraZ"
                     /locus_tag="PLY56_06690"
     CDS             complement(3890..4315)
                     /gene="mraZ"
                     /locus_tag="PLY56_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ21203.1"
                     /translation="MFIGGYFHTLDDKGRVVIPPRFRNLLGERFIITKGLHGCLWILK
                     EDRWAVIQQRLQTESMNKELLVLQRFLSGGAAECVPDSQGRILVPPVLREYARIEREV
                     VIIGAVNRVELWGKEMWDDYNARLSDEAVEACLERVDLC"
     gene            4459..>5155
                     /locus_tag="PLY56_06695"
     CDS             4459..>5155
                     /locus_tag="PLY56_06695"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HOJ21204.1"
                     /translation="MEREQLLQLLAELLVTHSPPGDEREMDALLLPRFRESCAEVRQD
                     PAGNIIGRIPGRTREGAIQVQAHKDELGLIIKRIDADGKIQARPLGGCVPWKYGEGPI
                     DLLGPEGPIPAVLCVGSTHTSAETTRVQSARTGPLTWENVYLDAKLSREELAARGIRI
                     GSRAVMSRQRKAPLVLGDYVCGWGLDDKGAVAIMLGVMERLAPLSGDLPVDVYFAATS
                     EEEATDALTREDVW"
BASE COUNT          910 a         1741 c         1675 g          819 t
ORIGIN      
        1 gctgggatct cctctccgcc tacccgcgtg atgaccgcct gcagctctgc cgggagcacc
       61 ccaacgcgcg ccttgtccgg caaacgtgga accagcgagg ttggagggcc tatctcttcg
      121 cactggacgg cacggactga cgccggagag ctgataccgc ctgcgcctat gggcctgcgc
      181 cgattgggcc ggacacacgc ttgaggcgct gggacacctc ctcgcgaaag cgcctccggt
      241 cgatggcatg cccctcggcc tcggcgaggc tcaccttctc ctcaaccacc aggcgcacga
      301 gcgcatcgtg gagagagaag ccgaccgcgt caatgccatc ggccacctcc tcgaggcgcc
      361 ccgagcggat tgcatcgccg gtggcgggca tgacgatgaa ctcggacggc accgtcacgg
      421 tggccccgnn nnnnnnnngc tttccatcct tgctccggca gagggtctgc gcgaccgccc
      481 ccagcagcgc ttccccgagg cgttcatgga cttccgcgcg agtctccggg ccgaccaggg
      541 cgagcagccg gccgagggca gcctggaccg tgacaccatg gcagccggcg atgactagaa
      601 tgccggcgcg cgcggcatcg aggcacgccc acagcgtcgc ggcatccggg atctcatcga
      661 cgaagagaac ctccggcatg agcgtcatcg ccgcgaggat cgcttcaccg cggcggccgt
      721 ctgtgccgag gacacaccgg gtgagaagcg cgttttccgc aggaaccggg atctccacca
      781 gatcctcgat ggtgacaatg tgcgccaccc gcgcggcggc caccatgcat aggagagcgg
      841 tcaggagcgt ggtcagcccc tggcctggct gagccgcgac cagcaccagg ccggagcgcc
      901 ggtgaatcaa ctcgcggaaa cttgcgggca gatcatccgg caggctcacc tcctcgggca
      961 gcaggcggaa cacggccgcg ggacctgcct cgccccagag cgacgtgacc cgcgcgcgcc
     1021 cggcgcatgc cagcgaggtg aggaagcggc gcggcgagcc gtcttcgcca ggggtcgggg
     1081 cctcctgtcg caagaaggcg gcgagcatcc cgtcgggcat cgcgggagcg tttccaaggc
     1141 gttcggtgac gccctcgcgc ctctggaacg ctggagcgcc ttcggcaagg agcaactcct
     1201 ctgcgccggc ctcccgggcc atcgctgcga gatgctcgat atagttccgg ggggtgcgcg
     1261 ccacccggat ccaggatgcc tcttcgctct gctccgcggt gacgcgaaac tcgccggctc
     1321 cggtgcgctg gaccagctcg acccgctggc cggtagacca ggcatcgcgc tgggatactg
     1381 ggagaatctc gtggatgagc gcggccacct cccgcgcgct catcacttgg ttcagcgggt
     1441 gcgtgccggt cgcatccgtc aactgacagt tgtcatccga ggggatgaac aactcgtatc
     1501 caccttgatg gatgaggaac tcgaacagcg cttcgatctg ggccatccag ccacactccc
     1561 tcccacgcgc gctgccccca cccgccggca gcgtgcccca tgtcaccagg acagcagtcc
     1621 gtcctgcgca cgcccctatt ctatcacgaa tgcgcgcagg tggaaagcgg ggggctgcct
     1681 gagaccagcc ggatgctccc gcgcggcgcg ctaccggtgg gacgattcag gatgccgagt
     1741 caggcctctt ccggcatcgc ttgggccgcg gcggcgccag agaccgcgcg ccacgactca
     1801 taccatttcg gcgggctgaa cttgggcgcg aagatgtgcc gcaccggccc gaggcggtag
     1861 gcgtcgcgct ggaggcgctc ctgctcggcc cagaaggcat cggccagggc cagccggcgc
     1921 gcgtcccact ccccttccgg cccaatctcc cgatccaggc gttccagcgt cagcttgctg
     1981 cgcaggtgct ccaggcgcca tcccggcgtc gcctcgcgcg agagcgcctc gaactcctgc
     2041 gtggcggctg gcagatcgac cgcggcgcga tcgccccagg ccgccagcca cgaagcccaa
     2101 cgttcggcct gcgccggaga tgccccgaag tagcgcgcgc tgtaggcgcg caggacggca
     2161 ctcgcgtcgt caaactggcc gctggagagg ccagccacga gcgccttgtt tatatcatcg
     2221 aactgaccct cgctgtaggc ctgaaagccc tccgcgccca tcgtggcgat ctcgtccagc
     2281 gtggcggaaa gacgctgcga cgcgacgacg gcgccatgct tggcgtagat atccccattg
     2341 cctgggacgt cgccatagcc aatatggatg aaggcgagcc ggcggcaccc ctcgggtacc
     2401 ggaagatcgg caaatcgcgt ccgcccatat gggatgtgga agatgatcgc cttcgcccat
     2461 cccggcgcct cgcgcgcggc ccaatcgttg aacaggacgt gctcctcctc ggtccaccac
     2521 caggcgcaga gccacggctc gatctccgga tagtagcgct gtgccacggc gtggatatcg
     2581 cgcaccagcc gcgcccaggt gaggatccac ggggcgcagt ccgcgcaggc acatccaccg
     2641 taatcatagg caaacgcggt gaaggaatcg attcgaacgc cgctctcggc caggtctcga
     2701 aactggcggt cggcattctc cagcatgatc gtccgggctt ccggcttgct ggggcagagg
     2761 agctggccga agatcttggg ggccttggtg gctagcagat ccgggcggag ctgatcaagg
     2821 aagacgtggt ttggggtgag gatcaggtca ttcgccatcc ccagctcttg cgccgcgcgg
     2881 aaggccgcct tcttgcgtga gtacatctcc aggggcagat tccagtaggc gtcggtcgcg
     2941 taggggttgc atacatcggt acacgtcatc cagtcgccga agcggttgaa cccccagtgc
     3001 cgtatctcag cgaggtaggc ggccatctcg cggggccaca tcgcctcgta cgagttgccg
     3061 aagtgcccgg ggcagtaggc ttcacgaatg ggcagttgca tggtgcctcc aatcctgccc
     3121 attccttcca cggcggcctg actgcctcct gcccggcgaa gaggtgaggg cgggagcaac
     3181 cgctctcccg cccggccacc gtggcaagaa gactccggct gccctttttg ccccgggcaa
     3241 cccgccagtc atcagcttcg acggcggcgc tcttcagggg gcgcctcgcc accgagctgt
     3301 ccggccatac tggtttggcc ggcggccact cgcgtcgtga gtagcgacgt ggcgatgaac
     3361 aataggccaa cgagcaggtg aggcacccac acgttccacg tcgcacccgc gaacccaaag
     3421 agccacggag atatcgccag gaagataccc aggatgatat ccaaggtcag atgcgtgctg
     3481 agcgatatgt tccgatccat gcctggcgcg tactgcgtga agaggctgta cacgatcagg
     3541 accagaccgg taagaatcgc cacccatgtc gccgacgcaa ctccccagaa cccgaacagg
     3601 aacggcgcca ggacaagcac gatcccgacg acatagtcga ccgtccgatg tgtcagcgct
     3661 cggatcatct ctgcacctcc tctcctatgg agtcactttc ctctcctatc cttaccctcc
     3721 cgggccgatg cgaaacgaga tgagacgcgg gaattcccac tgcgcgagcg tccccaggcg
     3781 gtgagtgggg agccattgga tccgtgggct ttcgcggggg acatcggtag ttcggagcgg
     3841 agtgcgcggc cacgctcagg aaacaagggg ctggtgcatg gaaaggggat cagcagaggt
     3901 caacgcgctc gaggcaggct tcgacggctt catcgctgag gcgggcgttg taatcgtccc
     3961 acatctcctt gccccacagc tcgacgcggt tgacggcgcc gatgatcacc acttctcgct
     4021 cgatgcgcgc atactcgcgc aagacggggg gcacaaggat gcggccttga ctatcgggaa
     4081 cacactcggc ggcaccaccg gagaggaagc gctggaggac cagcagctct ttgttcatgc
     4141 tctccgtctg gagacgctgc tggatgaccg cccaccggtc ctccttgagg atccagaggc
     4201 agccgtgcag acccttggtg atgatgaagc gctcgcccag cagattgcgg aagcgaggcg
     4261 ggatcaccac ccggcctttg tcatccaggg tatggaagta gccgccgata aacacaggtc
     4321 gcctctccgg acgcgagtac agcgcccggt tcctggatta taccaaccgg gtgggggcgt
     4381 gtcaagcaaa cattgacacg ccgtgccgcg cgtgctaaga ttgaaggcgg gccaacccgt
     4441 tggcccggga ggaccacctt ggagagagaa caactgctcc agctcctcgc ggagctgctg
     4501 gtcacgcatt ccccgcccgg cgatgagcgc gagatggatg ctctccttct ccctcgcttc
     4561 cgggaaagct gcgcggaggt ccgccaggac ccggccggga acatcattgg gcgcatccct
     4621 gggcgcactc gcgagggcgc gatacaggtc caggcccaca aggatgagct gggcctcatc
     4681 atcaagcgca tcgatgccga tggaaagatc caggcgcgcc ccctcggtgg ctgtgtcccg
     4741 tggaagtacg gcgagggccc catcgatctc cttggcccgg agggccccat cccggctgtg
     4801 ctctgcgttg ggagcacgca cacctcggca gagactaccc gcgtgcaaag cgcgcgcaca
     4861 ggaccgctca cctgggagaa tgtctacctg gatgccaagc tctcgcgcga ggagctggcc
     4921 gcgcgcggca tccgcatcgg ctcgcgtgcc gtcatgtcgc gccagcgcaa ggcgccgctc
     4981 gtacttggcg attacgtgtg cgggtggggc ctggatgaca agggcgcagt cgcaatcatg
     5041 ctcggggtga tggagcgcct tgccccgctc tccggcgatc ttcccgtaga tgtctacttc
     5101 gccgccacca gcgaggagga ggccaccgac gcactcaccc gcgaggacgt ctggg
//
LOCUS       DAOSCV010000215         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10164_length_5143_cov_3.156447, whole genome shotgun sequence.
ACCESSION   DAOSCV010000215 DAOSCV010000000
VERSION     DAOSCV010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10164_length_5143_cov_3.156447"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2570
                     /locus_tag="PLY56_06700"
     CDS             <1..2570
                     /locus_tag="PLY56_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21205.1"
                     /translation="GRPFRLSFQACYNRDLRRIHGHKELYWSRIRWLDAAGNEAGVNS
                     FTFGETAERWREHVVQGTIPKNARQAVLSIGFDSPNLPTGSYVCLDDLSLSILTDPPR
                     YVESGELISCPVRIPPGARLSYQAETPRDTRVLLRVRTAPDDAGAPGRWTGWMGPDGS
                     EKTAFPAAGAPLPAASRAHAWAQYRVHLLTSNPAATPVVRSVTLGAGTRKVTERAWSG
                     PDRRPPVLVRRSPGRTAQADAPVRFVLHDGEGSGIDPRSVRVFVDGRLVTRGLTREPD
                     GTYRYTPEQPFTPVGIQPGFGGWAKANYQGLLSISEGEPREPGGAPSLLLRGRTGDTA
                     FALTSPVVAVQGGATYHVRFWSRHTMPLDKAGEGSGSYSSGIFFLDAQGRPVGERVPI
                     DFGDADPQWHETQMSVVAPPEARAAYIRIGWDHPNIGPGEEVAFADLSFEGPPAQAPW
                     IPNLHQITVDASDLCGNALSDEMWLLIREPRTRGIVTLRDDGVTLIDGQPFFPIGIYA
                     VWKREHNQQSFDRAFQELKEAGFNMAHTYNSARTPEFREFLDAATRHGFKLFISARGG
                     ANTRDPASVLLDVVADEAEPALLAWYLADDTASHIGPEELRRIHQAVRDIDPAHITVQ
                     ADPVGSPENSRYADYVESTDGFLPELYPIRGDGPDQVPLIIRDMKTIAADLKRAGTTR
                     KAIWAIVQVFEGWGWPRYPTDAEVRAMTYLSLIHGATGMTYYTYGGHGKNHGATHDPR
                     VWANLKRVAGELAQLQNVLVERDPGVKVACEVVAGPARDALGYPSISTMVRRHSGFTY
                     LFTASSSAENVKARFRVPGLGQKVECLFEERALSPQGGLLEDSFEPYGVHVYRWRNP"
     gene            2689..>5143
                     /locus_tag="PLY56_06705"
     CDS             2689..>5143
                     /locus_tag="PLY56_06705"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOJ21206.1"
                     /translation="MRLVWAIALCIFLGAGAPLSAATVLFDFETDEEVTLWHDERKTT
                     LGADKRLERSQAFAASGQFSMRFWTGKWRPEEHGGARKWPAFEGKPPITDWSRYDRLV
                     MELVNVTDTPHRLMLFITDSEKPTRHGLSHREMLPPHTRIQAVIPVRKGFADRKVDAS
                     DIQVMHFFTEDPPEEMVIHIDRLLLLEPGEEIPPLPASYLKELATLQAPNVEKARADL
                     GEAAGRLEEAARGKPRLAEWATKEIAGLRKELSRVEAALAQGDEAVLEAPKALARLRE
                     ETARLESLLALRVRFERIRPAVQVGPAEDVVVGFASSMEKVLPRAGAVSPQIRERVQI
                     ALARNEKESLQVVVLPCERDLKRVQVRARDLFAGPGRRFSARNIQAVPVGYVETKATP
                     PYGSPHIGWWPDPILDFMKDADVAKGDAQAFWVRVRAPKSQAPGLYHGKLEVLVEGRP
                     RFAFDLAVQVYGFTMPDASPLPLAITFSPHDHPTDQTKEVQAKWREMEEYPVNAWRRY
                     RSRWADFLADYYITYDSLYEYSGWEPNFEDLARLHRQGRLGRFNLGYFPVYTEDGWPD
                     VIKRIRARYEKAKALGLLEYAYIYGCDEHPKEKFPGVERAAAELKKEFPDVMVMTTTY
                     DHSFGLESEIQSVDAWCPLTSRFDPERAAAARARGKEVWWYICCGPHHPHANMFIEYP
                     AIEGRLLMGALTAKYRPDGFLYYQISIWNSRKPITGGPFTDWDPRSWTTYHGDGSWTC
                     VGPGGTPLPTIRLENFRDGLEDYAYARILEATIAKVEASPQLHERQAKWLETAKALLE
                     VPAEVAESMTRYTDDPAAVY"
BASE COUNT          856 a         1714 c         1760 g          813 t
ORIGIN      
        1 gcgggcgccc gttccggctc tccttccagg cctgctacaa ccgcgacctg cgccgtatcc
       61 acgggcataa agagctgtac tggtctcgga tccgatggct ggatgccgcc ggcaacgagg
      121 ccggcgtaaa ctcgtttact ttcggcgaga ctgcggagcg ctggcgcgag cacgtggtgc
      181 agggcaccat ccccaagaac gcgcgccagg cggtcctctc catcggcttc gactcgccca
      241 atcttcccac cggctcctac gtctgcctgg atgacctctc gctttccatt ctcaccgacc
      301 cgccgcgcta cgtggagagc ggcgagttga tctcgtgtcc ggtgcggatt cctccgggcg
      361 cgcgcctctc gtatcaggcg gagacgccac gagacacccg cgtactcctt cgggtgcgca
      421 cggccccgga tgacgccggc gcgcccgggc ggtggaccgg ctggatgggt ccggatggga
      481 gtgagaaaac cgcgttccca gcggccggcg cgccgctgcc ggccgcgtcg cgcgcgcacg
      541 cctgggcgca gtaccgcgtt cacctcctta cgagcaatcc ggcggcgacg cccgtggtgc
      601 gctcggtgac gctcggcgct ggcacccgga aagtgacaga gcgcgcctgg agcggcccgg
      661 accggcgccc gccggtgcta gtccgccgga gcccggggcg aaccgcgcag gcggatgccc
      721 cggtgcgctt cgtgctccac gatggcgagg ggagcggcat cgacccgcgc tcggtgcgcg
      781 tcttcgtgga cgggcgcctg gtgacacgcg ggctcacgcg cgagccggac ggcacctacc
      841 gctacacccc ggaacagcct tttacccccg tggggatcca gcccggattc gggggatggg
      901 cgaaggcgaa ctaccagggg ctcctctcga tcagcgaggg cgagcctcgt gagccgggcg
      961 gagcgccctc gctgctcctc cgcgggcgca cgggcgatac ggcgttcgcg ctcacctcgc
     1021 ccgtggtcgc ggttcagggc ggggcgacgt atcacgtgcg cttctggtcg cgccacacga
     1081 tgccgctgga caaggcgggc gaggggagtg gatcctactc aagcgggatc ttcttcctcg
     1141 atgcccaagg ccggcccgtc ggggagcggg ttccaatcga ttttggcgat gcggacccgc
     1201 agtggcacga gacgcagatg agcgtggtcg cgccaccgga ggcgcgcgcc gcctacatcc
     1261 gcatcggctg ggaccatccg aacatcggtc caggggagga ggtggcgttc gcggatctct
     1321 cattcgaggg gcctcccgcg caggcgccat ggattccaaa cctgcaccag atcacggtgg
     1381 atgccagcga cctgtgcggc aacgcgctct cggacgagat gtggcttctg atccgcgagc
     1441 cgcgcacgcg cgggatcgtc accctgcgcg atgatggcgt gacgctcatc gacggccagc
     1501 cgttcttccc tatcgggatc tacgcggttt ggaagcgcga gcacaaccag cagagcttcg
     1561 accgcgcctt ccaggagctg aaggaggccg gattcaacat ggcgcatacc tacaactcgg
     1621 cgcgcacccc ggagttccgc gagttcctgg acgcggcgac ccgccacggg ttcaagctgt
     1681 tcatctcggc gcgcgggggc gccaacacgc gcgatccggc cagcgttctg ctggatgtgg
     1741 tcgccgatga agccgagccc gccctcctcg cctggtatct ggccgacgac accgccagcc
     1801 atatcggacc cgaggagctg cgccgcattc atcaggcggt gcgcgatatt gacccggccc
     1861 atatcacggt ccaggccgat ccggtcggct cgccggagaa ctctcgctac gcggattacg
     1921 tggagtcgac cgacggcttc ctcccggagc tctatcccat ccgaggggat gggcccgatc
     1981 aggtgccgct tatcattcgc gatatgaaaa cgattgccgc cgacctgaag cgcgccggca
     2041 ccacccgcaa ggcgatctgg gcgatcgtcc aggtcttcga gggctggggg tggccgcggt
     2101 atccgaccga cgccgaggta cgcgcgatga cctacctgtc gctcatccac ggggcaaccg
     2161 ggatgaccta ctacacctac ggcggccacg gcaagaacca tggcgccacc catgaccccc
     2221 gggtctgggc caatttgaag cgggtggccg gcgaactggc gcagctccag aacgttctgg
     2281 tggagcgcga tccgggagtg aaggtggcct gcgaagtggt ggccgggccg gcacgcgatg
     2341 ccttgggcta cccctccatc agcacgatgg tccgcaggca cagcggcttc acctacctct
     2401 tcacggcgag ctcgagcgcc gaaaacgtga aagcgcgctt ccgcgtgccc ggcctgggcc
     2461 agaaggtgga atgcctcttt gaggagcgcg ctctctcgcc tcagggaggc ctcctggagg
     2521 acagcttcga gccgtatggc gtccacgtct atcgctggcg gaatccatga gccgcaggga
     2581 agacggcgag atcccgccct cgaacgagcg gttcgagggc ccggccccgg cgggcaacgc
     2641 ggcccccgcg ggcgacgtat ctcatcgggt ggaaaggagt ggcgtgagat gaggctcgta
     2701 tgggcgatcg cgctctgcat ctttctcggc gcgggcgcgc cgctctcggc ggcgacggtg
     2761 ctgtttgact tcgagaccga cgaagaggtg acgctgtggc atgacgagcg caagacaacg
     2821 ctcggcgccg ataagcgcct ggagcgctcc caggcgtttg ccgcctcggg ccaattctct
     2881 atgcgcttct ggacagggaa atggcggccc gaggagcacg gcggcgcacg caagtggccg
     2941 gcgtttgaag ggaagccccc gatcaccgac tggtcgcgct acgaccgcct ggtgatggag
     3001 ctggtcaacg tgacagacac gccccaccgg ctgatgctct tcatcaccga ctcggagaag
     3061 ccaactcgtc acggcctgag ccatcgcgag atgctcccgc ctcacacgcg gatccaggcc
     3121 gtgatcccgg tgcggaaggg tttcgccgac cgaaaggtgg atgcatccga tatccaggtg
     3181 atgcacttct tcaccgagga cccgcccgag gagatggtga tccacatcga ccggctgctc
     3241 ctcttggagc cgggcgagga gatcccgccc cttcccgcga gttacctgaa ggagctcgcc
     3301 acgctccagg cgcccaacgt ggaaaaagcc cgcgccgatc tcggcgaggc ggcgggccgc
     3361 ctggaagagg cggcgcgcgg gaaaccccgc ctggcggagt gggcgacgaa ggagattgcc
     3421 ggcctccgga aggagctctc gcgtgtcgag gccgcgcttg cccagggcga tgaagcggtg
     3481 ctcgaggcgc caaaggcgct cgcgcgcctg cgagaggaga ccgccaggct agagtcgctc
     3541 cttgccctgc gcgtgcgctt cgagcggatc cgaccggcgg tgcaggtggg gcccgccgag
     3601 gatgtggtcg tcggctttgc gagctcgatg gagaaggtgc tgcctcgggc gggggccgtc
     3661 tcgccgcaga tccgggagcg cgtgcagatc gcgctggcgc gcaacgagaa ggagagcctg
     3721 caggtggtgg ttctcccctg cgagcgcgac ctaaagcgcg ttcaggtgcg cgcgcgtgac
     3781 ctctttgccg gcccgggaag gcgcttctcc gcacgcaaca tccaggcggt gccggttggc
     3841 tacgtggaga ccaaggcgac gcctccctac ggctcaccgc acatcggctg gtggccggac
     3901 ccgatcctcg acttcatgaa ggatgccgat gtggcgaagg gagacgcgca ggcgttctgg
     3961 gtgcgcgtcc gtgccccgaa gtcgcaggcg cccgggctct accacggcaa gctcgaggtc
     4021 ctggtcgagg gccggccgcg gttcgccttc gacctagcgg tgcaggtcta cggctttacc
     4081 atgccggatg cctcgccgct tccgctggcg atcaccttct cgccccacga tcacccgacc
     4141 gatcagacaa aagaggtgca ggcgaagtgg cgggagatgg aagagtaccc ggtcaacgcc
     4201 tggcgccggt atcggtcgcg atgggccgac ttcctggcgg actactacat cacctacgat
     4261 agcctctacg agtactcggg atgggagccc aacttcgagg acctggcgcg cctgcatcgc
     4321 cagggccggc tcgggcggtt caacctcggc tatttccccg tctatacgga ggatggctgg
     4381 cccgacgtga tcaagcggat ccgggcgcgc tatgagaagg cgaaggcgct cggtctcctg
     4441 gagtatgcct acatttacgg gtgcgacgag catccgaaag agaagttccc tggcgtggag
     4501 cgtgcggctg cggagctcaa gaaagagttc cccgatgtca tggtaatgac caccacctac
     4561 gatcacagct tcggcctgga gtcggagatc cagtcggtgg atgcctggtg cccgctgacg
     4621 tcgcgctttg atccggagcg agcggccgcc gcgcgtgccc ggggcaaaga ggtgtggtgg
     4681 tacatctgct gcgggccgca ccatccccac gccaacatgt tcatcgagta cccggcgatt
     4741 gagggccggc tcctgatggg cgcgctgacg gcgaaatacc gcccggacgg cttcctctat
     4801 taccagatca gcatctggaa ctcgcggaag ccgatcaccg ggggcccctt caccgattgg
     4861 gacccgcgga gctggacaac ctaccacggc gacggctcct ggacgtgcgt tggtccgggc
     4921 ggaacgccgc tgcctaccat ccgcctggaa aacttccgcg atggcctgga agactatgcc
     4981 tacgctcgga ttctcgaagc cacgatcgcg aaggtggaag cctcgcccca gctgcacgag
     5041 cgccaggcca agtggctgga gacggccaaa gctctgctcg aggtgcccgc ggaagtggcc
     5101 gagagcatga cccgctacac cgacgacccc gccgcggtgt acc
//
LOCUS       DAOSCV010000216         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10171_length_5141_cov_2.816359, whole genome shotgun sequence.
ACCESSION   DAOSCV010000216 DAOSCV010000000
VERSION     DAOSCV010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10171_length_5141_cov_2.816359"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLY56_06710"
     CDS             <1..379
                     /locus_tag="PLY56_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21207.1"
                     /translation="RASALLGGTIGTAIKVFSIGYAVRVFGPQINSFINSVMAQRGIE
                     REGGTKVVPILSVGQGLYIGAAQVMGPASRVDNVRSVAQGEVTLGDVRLNGLFPLNAT
                     VPLISPTSTVPGVGISAIIDFDV"
     gene            402..1115
                     /locus_tag="PLY56_06715"
     CDS             402..1115
                     /locus_tag="PLY56_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21208.1"
                     /translation="MEKRRAIALAAIVLMVTAVGLASGVGQMRAALAQPSLINVSARE
                     RQVDGQSQGEVLVGNQVVLRIRSSAGGLSAMQRANQVAQRLQAFLRSQQDVTAADFRV
                     ARAGNEWAVFAGDTLLITADAQQAALNGTTPERLARSWRDNIAQAVTTQVAGYRAEAE
                     RSSSKIVPIVSLGSGTRVGAAQITGPATNVAQASVVGQIETTFQDVVRIRIFVPLESV
                     TNIQRVPQVNVSAYGDIRL"
     gene            complement(1094..3865)
                     /locus_tag="PLY56_06720"
     CDS             complement(1094..3865)
                     /locus_tag="PLY56_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOJ21209.1"
                     /translation="MQSRGRDTLRRATGQMLGARVVPTRRTYVAVPLRLRVPALLIGL
                     WLALAAWPASAAYVSRVTATRIEVERLPRHTEPGSFLLAGPETAPRGYLRVVEVKGRR
                     AQCRLVAGTVHPGDETEPIASRPVRLALLTDNPQAPAIAELRTLFPGVAILGPEGRGL
                     DASRFDAAISLLYHPESIPLFRFAAMAAFASAGRPVFMGLSEYASRRGISVSVDRQEA
                     PHRVRCARTHPLLAGLAVGDELHWHGREGSGYVQRRLTAMPEGASPLLVPVGSARGAV
                     AVVEEVGRRGGFIAAFDFISPNGQPGVDPGATWKWLPVGNALHRSIRFARSLAQRQSY
                     DELVERMRDLERRFPGRVALQPLGNDAAGTPIFGLRLGDVSRPAFLIQAGIHGAEWVN
                     TAALLRLAEVLATPQDDPRPRWILAHFSVLLVPVMSPAEYARGAPPGETGRDLECNYD
                     YQWEELAGTLADEWRSALPAERLKGPRPWSEREARILRDLLVNQPVIGYISLHMHGEK
                     RGWRMLFPSTNAAHHTPLLDDLCALVNARLRGRFLRGAEQVWLQPEEWRDEPSSFNWA
                     SVRRIPACALETTGTGEQTAITTEVVNEAALGFMEVVAAHALARPQPWAVAGRREIEH
                     PAGRSDLIAFLFGQGSRLRVAYWKHRGVGVLRMLPLPHEAFLRDMKGHVVAFSRTESG
                     LHLPVGEPLLFDPGGAPAEKVVRAFQEASLLPAPRQVIWIPADRARLFGRMQHASRLR
                     LKDPGALADWIVCTGKPDRDRMPSWYAEFAINIPATDTYTIWARLRSPSDQAGAFAIA
                     APGTQISRDPALLLGNTDGGVTHWHWASQGSEEDTPPGSDRLRLRLTRGRMLLRVYAR
                     EAPGTAETNPRLDFLCLTNDPDYTPSDADVPQALRKDQAKRTSLGPPASPSPPARRVP
                     SDGYSRISP"
     gene            complement(3895..>5141)
                     /locus_tag="PLY56_06725"
     CDS             complement(3895..>5141)
                     /locus_tag="PLY56_06725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOJ21210.1"
                     /translation="VSSDSFSRNLRDTSRPVDSGGRPCYHPGVSINPNDPNTWPEAAR
                     ALLRRREYATTGRPELIALIRERIEAQGPLSFAEFMRLALYHPEHGYYTTPDLRFGRE
                     GDFLTAPETHPAFGALVCRFLARLWSRMGAPARFTVVEAGAGTGALAAQIWRYAAERH
                     PDLAAALEYLLVETSPSLRARQQRALEAVAANARWLESLEEIPPGSVEGCLLSNELLD
                     ALPVHRVQMTARGLQELYVGLGDGEFCDLPGPLSMPEIEQFFVQVGVMPPEGAIAEVG
                     LEGVAWYRQAALCLGRGVLLTIDYGYPAAELYTPERPRGTLLCYYRHTTNEDPYRRIG
                     TQDITAHVDFTALERAGEEAGLQTRALQTQREWLHSLGIEEWLDGRERWPAGEWPRRD
                     LIALVHPGALGRLRVLEQAKRL"
BASE COUNT          838 a         1723 c         1795 g          785 t
ORIGIN      
        1 cagggcgagc gctctcctcg ggggcacgat tggcaccgcg atcaaggtgt tcagcatcgg
       61 gtacgcggtg cgcgtctttg gcccccagat taactccttc atcaacagcg tcatggctca
      121 gcgcggcatc gagcgcgaag ggggcaccaa ggtggtgccg atcctctccg tggggcaggg
      181 cctctacatc ggcgccgcgc aggtgatggg cccggcgtcg cgagtagaca atgtgcgctc
      241 cgtcgcccag ggcgaggtaa ccctcggcga tgtccgcctc aacgggctct tcccgctgaa
      301 tgcgacggtg cccctgatca gccctaccag caccgttccc ggggtgggca tctcggcgat
      361 cattgacttc gatgtctaga ccagggcgag aggagaaggt catggagaag cgcagagcga
      421 ttgcgctggc cgccatcgtg cttatggtga cggccgtggg gctcgccagc ggtgtcggac
      481 agatgcgcgc cgcgctcgcc cagccgtcgc tgatcaacgt gtccgcgcgc gagcgccagg
      541 tcgatggcca atcgcagggc gaggtgctgg ttggtaacca ggtggtgctc cggatccgct
      601 cgagcgccgg cgggctatcg gcaatgcagc gcgccaatca ggtggcgcag cgcctgcagg
      661 cattcctccg gtcacagcag gatgtgactg cggccgactt tcgcgtggcc cgggcgggta
      721 acgagtgggc cgtctttgct ggcgacacgc ttctgatcac cgccgacgcg caacaggcgg
      781 cgctcaacgg taccacgcca gagcgtcttg cacggtcctg gcgagacaac atcgcccagg
      841 ccgtgacgac ccaggtcgcg ggttatcgcg ccgaggccga gcgctcgtcg agcaagatcg
      901 ttccgattgt cagcctgggg agcgggaccc gtgttggggc ggcacagatc accggcccgg
      961 caaccaacgt agcccaggcg agtgtcgtgg gtcagatcga gaccacgttc caggatgttg
     1021 tccgcatccg catcttcgtg ccgctggaat cggtcacaaa tatccagcgc gtgccgcagg
     1081 tgaacgtctc ggcctacggg gatatccggc tgtagccgtc cgatggaacg cgccttgccg
     1141 gaggagatgg tgaagcgggc ggccccaggg acgtccgctt cgcctggtct ttccgaagcg
     1201 cctgcggcac atctgcgtcg gagggcgtgt agtccgggtc attcgtgagg cagaggaagt
     1261 caagccgagg gttggtctcg gcggtgcccg gggcctctcg cgcatacacg cgcagcagca
     1321 tcctccctcg cgtcaagcgg agccgcaggc gatccgaacc cggcggcgtg tcctcttcag
     1381 atccctggct ggcccagtgc caatgggtca cgccgccgtc ggtgttgccc aggagaaggg
     1441 cgggatctct cgatatctga gtgccgggag cggcaatggc gaaggcgcct gcctggtccg
     1501 atggcgagcg caggcgtgcc cagatggtgt aggtgtccgt cgccgggatg ttgatggcaa
     1561 attcggcgta ccaggatggc atccggtctc gatccggctt tccggtgcag acaatccaat
     1621 ccgccagggc tccgggatcc ttcaggcgga ggcgagacgc gtgttgcatg cgcccgaaca
     1681 ggcgggcgcg atccgcgggg atccagatca cctggcgcgg tgccggcaag agggatgcct
     1741 cctggaaggc gcgcacaact ttctccgccg gagcgccccc gggatcgaag aggagaggct
     1801 ctcccacggg gaggtggagc ccggattcgg tgcgcgagaa ggcgacaaca tgccccttca
     1861 tgtcacgcag gaacgcctcg tggggtagcg gcagcatccg gagcactccc accccgcggt
     1921 gcttccagta ggcgacgcgc agccggctcc cctggccgaa gaggaaggcg atcaggtcgc
     1981 tccggcccgc cgggtgctcg atctcgcgcc ggccggctac ggcccacggc tggggacgcg
     2041 ccagagcgtg cgccgcgacg acttccatga agccaagagc ggcttcattg accacctccg
     2101 tggtgatcgc cgtctgctcc ccggtgccgg tggtttcgag ggcgcacgcg ggaatccggc
     2161 gaacgctcgc ccagttgaac gaagagggct cgtcccgcca ctcctcgggc tgcagccata
     2221 cctgctcggc gccccggagg aaacgtccgc gcagacgggc gttgaccagc gcgcagaggt
     2281 catccaggag tggggtgtga tgcgctgcgt tggtgctcgg gaagagcatg cgccacccgc
     2341 gcttctcgcc gtgcatatgg agcgagatgt agccgatcac cggttgattc accagcaggt
     2401 cgcggaggat gcgcgcctct cgctccgacc acggccgtgg tcccttgagc ctctcggccg
     2461 ggagcgcgct ccgccattcg tcggcgagcg tgccggcgag ctcctcccac tggtaatcgt
     2521 agttgcactc caggtcgcga ccggtctcgc ctggcggcgc tccgcgcgca tattcggcgg
     2581 ggctcatcac gggcaccaga agaacggaaa agtgcgcgag aatccagcgc gggcgcgggt
     2641 cgtcctgggg tgtcgcgagc acctctgcca ggcgcaagag cgcggccgtg ttcacccatt
     2701 cggcgccatg aatgccggcc tgaatcagga aagcgggccg cgagacgtct cccaggcgca
     2761 ggccaaagat cggggtgccc gccgcatcgt tcccgagcgg ctggagtgca accctccccg
     2821 gaaagcggcg ctccagatcc cgcatccgct ccaccaactc atcgtaagac tggcgctgtg
     2881 ccagcgagcg tgcgaagcgg atgctccggt gcagcgcgtt gcccaccggc agccacttcc
     2941 atgtggcgcc ggggtctacc ccgggctggc cgttgggcga gatgaagtcg aacgccgcga
     3001 tgaagccgcc gcgccggcct acctcctcga ccaccgccac cgcgccgcgg gccgatccca
     3061 cggggaccag cagcggcgat gcgccttcgg gcatggctgt cagccggcgt tgcacatagc
     3121 cgctgccctc gcggccatgc cagtgtagct cgtcgcccac cgccagcccg gccagaagcg
     3181 ggtgcgtccg cgcgcatcgg acgcgatggg gcgcctcctg gcgatccacc gatacggaga
     3241 tcccgcgtcg cgatgcatac tccgagagcc ccatgaagac cgggcgtccg gcagaagcga
     3301 acgccgccat cgcggcaaac cggaacaacg ggatcgattc tgggtggtag aggagggaga
     3361 ttgccgcgtc gaaccgcgag gcatcaaggc cccggccctc ggggccgagg attgccactc
     3421 cagggaagag ggtgcgtagc tccgcgatgg ccggcgcctg cggattgtcc gtgaggagcg
     3481 ccagcctgac ggggcgcgac gcgatcggct cggtctcgtc acctgggtgg accgtgccgg
     3541 ctaccagccg gcactgcgcc cgacgcccct tgacctccac gacccgcagg tatccccggg
     3601 gcgccgtctc gggtcccgcc agcaagaagc tgcctggctc ggtgtggcgg ggaaggcgct
     3661 ctacttcgat ccgcgtcgcg gtcacgcgcg agacgtaggc agcggatgca ggccaggcgg
     3721 ccagcgccag ccacaagccg atgaggagcg caggcacgcg cagccgcaat ggcaccgcca
     3781 cgtaggtgcg gcgtgtcggg actacccgcg cgcctagcat ctggccggtc gcacggcgga
     3841 gggtatcgcg acctctgctc tgcatacagc ctgcctccgc ggtaccattc ggcgtcagag
     3901 gcgtttcgcc tgctccagca ctctcaatcg gcctaaggca cccggatgca ccagggcgat
     3961 cagatcccgg cgcggccact ccccggcggg ccagcgctcg cggccatcca gccactcctc
     4021 aatccctagg gagtgcagcc actcgcgctg tgtctggagc gcgcgcgtct ggagcccggc
     4081 ctcctcgccg gcgcgctcca gcgccgtgaa atcgacgtga gcggtgatat cctgggtgcc
     4141 gatgcgccgg tacggatcct cgttggtcgt gtggcggtag tagcagagga gcgtgccgcg
     4201 gggccgctcg ggcgtgtaga gctcagcggc gggatagccg tagtcgatag tgagcaacac
     4261 tccccgcccc aggcagagcg ccgcctggcg gtaccaggcc acaccttcga gaccgacttc
     4321 agcgatggcg ccctcgggtg gcatgacgcc cacttggacg aagaactgct cgatttccgg
     4381 catggagagc ggtccgggaa ggtcacagaa ctcgccgtcg cccagcccga cgtacagctc
     4441 ctgcaatccg cgggcggtca tctgcacccg atgcacgggc agggcgtcca gcagctcgtt
     4501 gctgaggaga cacccctcca cgcttccggg gggaatctcc tcgaggctct ccagccaccg
     4561 ggcgtttgcc gcgacggcct cgagcgcccg ctgctggcgc gcgcgcagcg aggggctggt
     4621 ctccaccagg agatactcca gcgcggcggc gaggtcgggg tggcgctctg cggcatagcg
     4681 ccagatctgc gcggcgagcg cgcccgtgcc cgctcccgcc tccaccacgg taaaccgcgc
     4741 cggcgcgccc atccgcgacc agaggcgcgc cagaaaccgg cagaccagcg cgccgaaggc
     4801 cggatgagtt tcgggggctg tcaggaagtc gccttcgcgt ccgaagcgga gatctggggt
     4861 ggtgtagtag ccgtgctctg gatggtagag cgccaggcgc atgaactcgg caaacgacaa
     4921 cggaccctgc gcctcgatcc gctcgcgaat cagcgcgatg agctcgggcc ggcccgtggt
     4981 ggcgtactcg cgcctgcgaa gcagcgcgcg cgccgcctct ggccaggtgt taggatcgtt
     5041 tggattgatg gacacacccg gatggtagca tggccggcca cccgagtcaa ctgggcgtga
     5101 cgtatccctg agattccgag agaaggaatc cgaagacacg g
//
LOCUS       DAOSCV010000217         5110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10238_length_5110_cov_3.705242, whole genome shotgun sequence.
ACCESSION   DAOSCV010000217 DAOSCV010000000
VERSION     DAOSCV010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10238_length_5110_cov_3.705242"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLY56_06730"
     CDS             <1..854
                     /locus_tag="PLY56_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ21211.1"
                     /translation="ERPFGITTHIGLLELSDMNRPLLRRLLQEAPGTYIHSLNVGQLA
                     EAAAEAIGADALLARVGAYYHDIGKVMRPYCFVENQTLENIHNRLNPTLSTLVVTSHI
                     KDGVELAREYRLPGVIQEIIRSHHGTGLVKYFYHQAMLASEGDHRVPEEQFRYEGPRP
                     RTKEAGIIMLADSVEGAVRSSLMERPDPARIQAIVTRIIKEKTGDGQLDECDLTFRDL
                     TKIGVTFTRILQGMFHSRIKYPEPLTPEGQKLDAIDNGHGSPAEHPAIPFDEVGDRAD
                     RREAAGT"
     gene            805..1230
                     /gene="ybeY"
                     /locus_tag="PLY56_06735"
     CDS             805..1230
                     /gene="ybeY"
                     /locus_tag="PLY56_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ21212.1"
                     /translation="MTRLEIERIAGKLLERENLPGEIEISVLFTDNEGIRQLNREYRG
                     IDTPTDVLSFPLSTPEELVQAPEETEVLLGDVVISVETAREQARQQGHSIKRESALLL
                     THGLLHLLGYDHGSEEERERMRQMESEVVAGAAYLDRAP"
     gene            1227..1931
                     /locus_tag="PLY56_06740"
     CDS             1227..1931
                     /locus_tag="PLY56_06740"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOJ21213.1"
                     /translation="MKSRTLLDSFRFAVEGVLHVFRTQRHMRFHFFMMVLVLVLAKIY
                     RLNRTELLVLFFSICFVLIAEMFNTAVEAVTDMITEAYHPLAKQAKDVSAGAVLIASI
                     NAVVVGFVLFLDDARIRQVLAGAYESSPMPIAYIAVIEVVMLLAVLVVLRVMGYSPTD
                     SRSPINGHAALAFSLATLIVLVAQQEVAVGVFALLLAALVVQSRLERGLQGFRAVFTG
                     ALLGISLPVILMRLLP"
     gene            2071..3546
                     /locus_tag="PLY56_06745"
     CDS             2071..3546
                     /locus_tag="PLY56_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ21214.1"
                     /translation="MGGLWLFLGPMLAVGAMLAAGYGIAAPGTAGPVPARPPRPFPWD
                     IVIAITAMILFVAFLAAAETALGSMRKTRLRQLIEDGVRAAQPAQRLLEEPARFVATV
                     QIGITLASMFAAAIAARAPMERLASWLGGLAREQGADWLVAYTFEASFVITILIVGFV
                     ELLLGELIPKQLAHRNPEPIALRVAGPIEAMAVIASPVVWVLTQLSSAVAGLFSGREE
                     VAAHMTEEEIKTIVEESEKEGVLEEQETEMIHSVLEFTDIVVREVMVPRIDMVVVEVT
                     RSIDDLLTVVLESGHTRIPIYEETVDNIVGIVHAKDLLRVVRQADPNTDIRSANVMRS
                     VYYVPENMKVDDLLAEFQRTKHQLAVVVDEYGGTAGLVTLEDLLEEIVGPITDEYDVE
                     NEPTISLVDENVALVDARMAVDDVNKELGIHLPEEESETLGGFVFGILGKIPTVGEVV
                     EYDDLVIEVIEADSRRVTRVKITRQPKPAESEAGDESSAAR"
     gene            4166..>5110
                     /locus_tag="PLY56_06750"
     CDS             4166..>5110
                     /locus_tag="PLY56_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21215.1"
                     /translation="MILFWCALPASLPFARSVAGAPQREVALVEECDPSFALRISFRV
                     AKPGGEAALMLRGPGGQGAYRLALSGSEIRLEEVGRARRRLARCAVRYAGANELLVVR
                     RESLLRVALNGEIRLERDDARIPIARVTLADPERRIVLLGEARYQPTEEIFLADDFMT
                     TPDAPSRWESVAGTWHVAGVSNPRAASDPFKFIGFASGEGMALSSESYWFWNDYRVAV
                     SSRLLGATSGMGLLFGHREGGSVYGLSWGGPGAAKGKANRLRLWRREGGRVSVLAERP
                     AYARVGQWYRLSVETQGAQITARIDENVVLTARDSRLLG"
BASE COUNT          881 a         1531 c         1739 g          959 t
ORIGIN      
        1 tggagcgccc gtttgggatc acgacgcaca ttgggctgtt ggagctatcc gatatgaacc
       61 ggccgttgct gcgtaggcta ttgcaggaag cgccgggaac ctatatccat agcttgaacg
      121 ttgggcagtt ggcagaggcg gcggcagagg ccatcggcgc cgacgcgctg ttggcgcggg
      181 tgggcgctta ttaccacgac atcgggaagg tgatgcgccc ctactgcttt gtcgaaaacc
      241 agacgctgga gaatatccac aaccggctga acccgacgct gagcaccctg gtggtgacga
      301 gccacatcaa ggatggcgtg gagctggcgc gcgagtaccg gctgccaggg gtgatccagg
      361 agatcatccg ctcgcaccac ggcaccggac tggtgaaata cttctaccat caggcgatgc
      421 tggcgagcga aggcgaccat cgggtgcccg aggagcagtt tcggtatgag ggcccccgtc
      481 cgcgcaccaa agaggccgga atcatcatgc tggcggactc ggtggagggg gccgtgcgct
      541 catcgctgat ggagcggcca gacccggcgc gcatccaggc gattgtgacg cggatcatca
      601 aggaaaagac cggggacggc cagctggatg agtgcgacct gacgtttcgc gacttgacga
      661 agatcggcgt gacgtttacg cgcatcctcc agggtatgtt ccattcgcgg atcaagtatc
      721 cagagccgct gacgccggaa gggcagaagc tagatgccat cgataacggt catggttcgc
      781 cggcagaaca tcccgcgatc ccctttgacg aggttggaga tcgagcggat cgccgggaag
      841 ctgctggaac gtgagaacct gccgggggag atagagattt ccgttctctt taccgataat
      901 gaaggtatac ggcagctaaa tcgtgaatac cggggaatag acactcctac cgacgtgctc
      961 tcctttccac tgagtacgcc cgaggagctg gtacaggcgc cggaagagac agaggtgctg
     1021 ctgggcgatg tggtcatctc agtggagacc gctcgcgagc aggcacgcca acaagggcac
     1081 tccatcaaac gggagtcggc tctgctcctg acgcacggac tcttgcacct gctgggctat
     1141 gaccacggga gcgaggagga gcgggagcgg atgcgccaga tggagagcga ggtcgtggcc
     1201 ggggccgcat acctggatcg cgcgccatga agagccgcac gttgctcgat agtttccggt
     1261 tcgccgtcga gggagtgcta catgtgttcc gcacgcagcg gcacatgcgt ttccacttct
     1321 tcatgatggt gctggtgctt gtgctggcga agatctaccg gttgaaccgc acggagctgc
     1381 tggtgctctt tttcagcatc tgctttgtgc tgatcgccga gatgttcaac accgccgtgg
     1441 aggcggtgac cgatatgatt accgaggcgt accatccgtt ggcaaagcag gccaaggatg
     1501 tgagcgccgg cgcggtgctc atcgcctcga ttaacgccgt ggtcgtgggg tttgtgctct
     1561 tcctcgatga tgctcggatc cggcaggtgc tcgccggggc ctacgagtcc tcgccgatgc
     1621 cgattgcgta tatcgccgtc atcgaggtgg tcatgctgtt ggccgtcctg gtggttctcc
     1681 gggtcatggg ctacagcccc accgattcac gcagtcccat caatggccac gctgctctcg
     1741 cgttctcgtt ggcgacgctc atcgttctgg tggcgcagca agaggttgcc gtgggagttt
     1801 ttgcgctgct gctggcagcg ctggtggtac agagccgcct tgagagaggc ttgcagggct
     1861 tccgggcggt cttcaccgga gcgcttcttg gaatctcact tccggtgata ttgatgcggc
     1921 tgctgccata ggaaaggaga gcgtttgggt tcgaccgaac cccccgggaa acgcatagcg
     1981 atgtcgcctg cgcgggcgac tcccgacggg gtacagtctc aagatggccg tatttcctcg
     2041 cgccatggga aatcggatga gaaggcagca gtgggagggc tctggctctt ccttggccca
     2101 atgctggcgg tgggggcgat gctggccgca ggctatggca tcgcggcgcc agggaccgcc
     2161 ggccccgtgc cggcacggcc cccgcggccc ttcccctggg atattgtcat cgccatcacg
     2221 gcgatgatcc tcttcgtcgc cttcctggcg gcagccgaga cggctctcgg cagcatgcgc
     2281 aagacgcggt tgcgccagct aatcgaggat ggcgtgcgcg cggctcagcc ggcacagcgc
     2341 ctcctggagg agccggcacg ctttgtggcg acggtgcaga tcggcatcac gctcgccagc
     2401 atgtttgctg ccgcgattgc cgcgcgcgcg ccgatggagc gcctggcaag ctggctgggc
     2461 ggcttggcgc gcgagcaggg tgccgattgg ctggtcgcct acaccttcga agccagcttc
     2521 gtcatcacca tcctgatcgt tggcttcgtg gagctcctcc tcggcgagtt gatccccaaa
     2581 cagctcgctc accgcaaccc cgagccgatc gcgcttcgcg tcgccggccc catcgaggcg
     2641 atggccgtca ttgcctcgcc cgtagtctgg gtgctcactc agctgagcag cgctgtcgcc
     2701 ggcctcttct cgggccgcga ggaagtcgcg gctcatatga cggaagaaga gatcaagacg
     2761 atcgtcgagg agagcgagaa ggagggcgtc ctcgaagagc aagagacgga gatgatccac
     2821 tccgtgctcg agttcaccga cattgtcgtc cgcgaggtga tggtgccccg aatcgacatg
     2881 gtggtcgtcg aggtgacccg aagcatcgat gacctcctca ccgtagtcct ggagtcgggg
     2941 catacgcgca ttccaatcta cgaagagacg gtcgataaca tcgtcggtat cgtccatgcc
     3001 aaggacctct tgcgcgttgt gcgccaggcc gatccgaata cggacattcg ttccgccaac
     3061 gtgatgcgct cggtctacta cgtgcccgag aacatgaagg tggatgatct cctggcggag
     3121 ttccagcgca cgaagcacca gctagccgtc gtcgtggatg agtatggcgg cacggccggc
     3181 ctggtgacgc tggaggatct cctcgaggag attgtcgggc cgattaccga cgagtatgac
     3241 gtggagaacg agcccacgat ctccctggtc gacgagaatg tcgccctcgt ggatgcgcgc
     3301 atggcggtgg atgatgtgaa caaggagctg gggatccacc ttcccgagga ggagagcgaa
     3361 accctgggtg gctttgtctt cggcatcttg ggcaagatcc ccactgtcgg cgaagtggtt
     3421 gagtatgatg acctcgtgat cgaggtgatc gaggccgata gccggcgtgt cactcgcgtg
     3481 aagatcaccc ggcagccgaa gccggcggaa tcagaggcgg gtgacgagtc atctgccgct
     3541 cgctagagat cgatctgccc cgggggcgat catgacactc ccggggcagc acccactctt
     3601 gccatgacta ggaatccggt ctgggcacag agacttccgg ctccaatgcc cggatctctc
     3661 cggcctcctc tcccatagcc ccgcgggcag ccctgcccgg ccccttcgcg gtcctccgcg
     3721 agcccaacgc ggtgggccat gccccgatcc cggatgggtc gcacatcccc aggcgcccgc
     3781 gcctcgccat ccgtgttcgc ccccttcatc cgcggtatcg aaggcaggac gcgggcggga
     3841 tatcggtggc gacgcgcggc gatagatcac cgcgctgctt gtgagcgaag ccctgccggg
     3901 ctggattgga cgacaccctc gggggcctca taacggcagg gtctgggatg gggggctccg
     3961 aaggggcttt gccgagaagc agcgcggagc ttcgagcacc gcgcctgggt gagcatcccg
     4021 cccggtcctc ttcgaaacaa aacgtggtct ggcggcgcct aagagaggag cgcgtcccgc
     4081 acgggggcgc tatttcgcca tggggagact ggtcagggga atgcggtttc gaacgagcct
     4141 tcaactgcta cggagatcgc cgctgttgat cctgttctgg tgcgcgttgc ccgcctcgct
     4201 gccttttgcc cggtcggtcg caggggcgcc gcaacgcgag gtggcgttgg tggaagagtg
     4261 cgatccctcc ttcgcgcttc ggatctcctt tcgcgtggca aagcccggcg gcgaggcagc
     4321 gctgatgctg cgcggcccgg gcgggcaggg agcgtatcgg ctcgctctct caggatcgga
     4381 gatccggttg gaggaggtgg gtagggcacg ccggcgcctg gcccggtgtg ctgtccgcta
     4441 tgccggcgcg aatgaactcc tcgtggttcg ccgcgagtca ctcctgcggg tggccctcaa
     4501 cggtgagata cgcctcgagc gcgatgatgc acgcatcccg attgcgcgag tgacgctggc
     4561 cgacccggaa cgccgaatcg ttctccttgg ggaggcgcgc taccagccca cggaggagat
     4621 ctttctcgca gatgacttca tgacgactcc cgacgcgccc tcgcgctggg agagcgtcgc
     4681 cggcacatgg catgtggcgg gcgtgtcgaa ccctcgggcc gcctcagatc ccttcaagtt
     4741 catcggcttc gcgagcggcg aggggatggc gctcagcagc gagtcttact ggttctggaa
     4801 tgactatcga gtggccgtct cttcccggct tctgggcgca acgtctggca tgggcctcct
     4861 ctttgggcat cgcgagggcg ggagcgtcta tggcctctcg tggggcgggc cgggcgcggc
     4921 aaagggaaag gccaaccgcc tgcggctgtg gcgacgggag gggggccggg tgagcgtgct
     4981 cgccgagcgg cccgcttacg ctcgcgttgg ccagtggtat cgactctcgg tggagaccca
     5041 gggggcccag atcacagcgc gcattgatga gaacgttgtc ctgacggccc gagactcgcg
     5101 cctcctcggc
//
LOCUS       DAOSCV010000218         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10268_length_5102_cov_3.825243, whole genome shotgun sequence.
ACCESSION   DAOSCV010000218 DAOSCV010000000
VERSION     DAOSCV010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10268_length_5102_cov_3.825243"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="PLY56_06755"
     CDS             <1..1704
                     /locus_tag="PLY56_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21216.1"
                     /translation="YQIALTRSLERQPDGTMAPASYWNQWEMESQDLRGAGLDQIAEN
                     PRRDNLYRLVRLEYLPSAQDPTTAQPEVRPYLLRAILPSDFDPSQPDQFREPGWLVDE
                     SLFPRKPKGTGIPPLCNSVAVANRRWRLEENGLLVGLTPKEDVDAAWVTYTPPPSGSP
                     AGTPGTWSAKPLIRFEPLAVVNEALDPVTQPGASYPATYRADYGHWQWKRRKAWSHPG
                     YGTSSQLPAWARYFQVFSIEVRRRYGNQPAVTYYVSAGRTDSASGVPATDTSHYYVWR
                     LTDREPVDGAWPASDQPTFDMTRYKARIEAFRQGPPSSDQQLAFWQSTNPYDTNLLPS
                     PGGTEPYWPEMAFLADVERGSINFRVDAPVRPPVANPVTSAFEPQPPGPLAPFQAPYF
                     GTGPDAVPAPDGERTDWLIPDVARAATSSNYRNSDGTTWIRIVPETMRVMVGQPINGD
                     PAQGYTWSVFTRVAEGTLPGRGQFCYDENYGLLTFAEEDASLWDGNTKIRAWYQLQTN
                     VQRAYASSPINSADGISVSASYLTKEVIRFTLGLRGYDKDTGKAHPFQLTSTIRPRNL
                     R"
     gene            1784..4978
                     /locus_tag="PLY56_06760"
     CDS             1784..4978
                     /locus_tag="PLY56_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21217.1"
                     /translation="MSPAAAGEKTLSATRAGQRARREEAGTGTIALVHTHSRQSRTSS
                     RSGQALILAVVIMLLLLFLGTIFLRTVLANLGHAEESVAAANEYSAAEAGIRFADTNL
                     TTSELGADWRPRASYSPGVVRPDDPDYYWIQPFDNKGDNDPSNDVGGYVRYNLGDSRF
                     LLRVTYNPNNDPRAPVMPDAKVVNRPSALSRFIMIEVVGRRGSAAEIDKEYEDAKHGD
                     PIDPTIFKATGAEASHRFRRELIAFKAIGINNYLRFVTDRNRLGRTTYFGVDQMFIRD
                     RAGSVPQNPPQPDAEVDYPFRLGIDPAGFTNTDGIAEWARATGSPIRVNGNLVWYGSA
                     PSTTGGLPEITLNLLDPRDQPWNVVEVAGKISREGRNQWKHPDRMALRVYDPSQAGPG
                     ELPLNYTSQTPLPTSDDDAAFAAVLEGSQSPALTVADCYLDNKWLTAEPQEKQGVDPR
                     RSIRRIEPPQIETVEPGTGLLRYQAMTYGAPAVTGSTAGASVSWQARLAARYGYGAGV
                     YIDNARHNSNHTRCQGDVQFNHSAEKLRDDWMRPYSETPQGSGSGWKHPGRVYEPVGT
                     LIILEPDVTVPGGPEKLGAGLMSITRADYRGWRSPTGERLPVQTVYFEIQPGTVASGV
                     QEYTLLPKIWSGSQWQTWDWTSRTWVPYDGTNGTRYPFNGVIFAEGNVRIRGRLPRDT
                     EHGGMRLTVVSRGTIYLEGNLLKGDPAPGMAPQSFLAVLARDYVCLNTTQFVQVQTDF
                     VPPTEGSIPPFFWRLPNNRPDLEFQASWDFGVPPTDESVQTYYGLSDPSNPDLNLCPA
                     LFLRHQADRDPVSIQWHLNNHPYRHYLYEHGYDSSVAQDFYPLFHVGEWEVFRDLVSL
                     MPFPLFPEPRAGSDPIAHRMPGKRNIIRIENYDPGQAPDGTATTGGDYNLGAIAVQPL
                     DIWIQALIYAEHNSWFVIPGVPFQDTDAGDPEPAISPRYPRNGEPLDVQIHIWGAIAE
                     GQAASLEDVTQWTARWRGANRTAYEAYGDPALTVDPTFTKRGGINYAFDPKLITDTRW
                     VRFAQGEPMHQLPALPRLPVCPDLVYFGERPVQE"
BASE COUNT          918 a         1652 c         1689 g          843 t
ORIGIN      
        1 tatcagatcg cgctcacgcg gtcgctcgag cggcagccgg atggcacgat ggcgcctgcc
       61 tcctactgga accagtggga gatggagagc caggatttac ggggagcggg cctggatcaa
      121 atcgcggaga atccgcgccg ggataacctc tatcggctgg tccgcctgga gtaccttcct
      181 tccgcgcaag atccgacgac ggcgcagccg gaggttcggc cctatctcct gcgcgcgatc
      241 ttgcccagcg acttcgatcc ctcccagccg gatcagttcc gcgagcccgg ctggctggtg
      301 gatgagtcgc tcttcccgcg caagccgaag ggcacgggta tcccgccgtt gtgcaactcc
      361 gtggcggtgg ccaaccggcg ctggcgcctg gaagagaacg ggcttctcgt aggtctgacg
      421 ccaaaggagg acgtggatgc ggcctgggtg acttacacgc cgcccccgtc cggttcaccc
      481 gcgggaacgc cgggcacatg gtcggccaag ccgctgattc gattcgagcc gctggcggtg
      541 gtcaacgagg cgctggatcc ggtgacccag ccgggcgcgt cctatcccgc gacgtatcgc
      601 gccgactacg ggcactggca gtggaagcgg cgcaaggcgt ggtcgcaccc gggctatggc
      661 acttccagcc agttgccggc gtgggcgcgc tacttccagg tgtttagcat tgaggtgcgc
      721 cgccgctacg gaaaccagcc ggccgtcacc tactacgtca gcgccgggcg aaccgacagc
      781 gctagtggcg tgccggcgac ggatacgagc cactactacg tgtggcgcct gaccgatcga
      841 gagccggtgg atggagcatg gccggccagc gaccagccca ccttcgatat gacgcgctac
      901 aaggcgcgga ttgaggcgtt ccggcagggg ccgccgtcgt cggaccagca gcttgcgttc
      961 tggcagagca ccaacccata cgataccaac ctgctcccga gcccgggagg caccgagccc
     1021 tactggccgg agatggcctt cctggcggat gtggaacgcg ggagtatcaa cttccgtgtg
     1081 gatgccccgg tgcgcccgcc cgtggccaac ccggtcacga gcgccttcga gccgcagcct
     1141 ccgggcccgc tggcgccgtt tcaggcgccc tactttggca cgggccccga tgcggtcccc
     1201 gcgcctgatg gcgagcggac cgactggctg attcccgatg tggcgcgggc cgcgacctcc
     1261 tcgaactatc ggaacagcga tgggacgacc tggatccgga tcgtgccaga gacgatgcgt
     1321 gtgatggtgg ggcaaccaat caacggggat cccgcgcagg gatacacctg gagtgtcttt
     1381 acccgggtgg cagagggaac gctccccggg cggggccagt tctgctacga tgagaattac
     1441 gggctcctca ccttcgccga ggaagatgcg agcctgtggg atggaaacac caagattcgc
     1501 gcgtggtatc agctgcagac gaatgtgcag cgcgcctacg catcgagccc gatcaacagc
     1561 gccgatggga tcagcgtgtc cgcttcctac ctgaccaagg aggtgatccg ctttaccctc
     1621 gggctgcgag gctacgacaa ggatacgggc aaggcacacc cgttccagct gacgtcgacg
     1681 attcgcccgc gcaaccttcg gtgagaacgg gcctgagccg gagatgaacg gagaatggga
     1741 gtggcgaatg gctgccacca gaaatgatag gagagggccg cagatgagcc cagcagcggc
     1801 aggggagaag acgctgtcgg ccacgcgcgc cggccagcgc gctcggcggg aagaggccgg
     1861 aacgggcaca atcgcgctcg ttcatactca ttcgcggcaa tcccggacct cctcgcgctc
     1921 tggtcaggcg ctgatcctcg ctgtcgtgat catgctcctg ctgctcttct tgggcaccat
     1981 cttcctcagg accgtgctgg cgaacctggg gcatgccgaa gagagcgtgg ccgcggcgaa
     2041 cgagtactcc gccgccgagg cgggcattcg cttcgcggat accaacctga cgaccagcga
     2101 gctaggcgcc gattggcggc cccgtgcctc ctattcgcct ggcgtcgtgc gccccgatga
     2161 tccggattac tattggatcc agccgttcga taataagggg gataacgacc ccagcaacga
     2221 cgtgggcggc tacgtgcgct acaacctggg cgattcgcgc ttcctgttgc gcgtcaccta
     2281 taacccgaac aacgatccca gggcgccggt gatgccggat gccaaagtgg tcaaccggcc
     2341 ttcggcactc tcgcgcttca tcatgatcga ggtggtgggc cgcaggggga gcgccgcgga
     2401 gatcgataag gagtacgagg atgccaagca tggggatccc atcgacccga cgatcttcaa
     2461 ggcgaccggt gccgaggctt cgcaccgctt ccggcgcgag ctcatcgcct tcaaggcgat
     2521 tgggatcaac aactacctgc gcttcgtgac tgatcgcaac cgccttgggc ggaccaccta
     2581 cttcggcgtc gatcagatgt ttatccgcga ccgggctggc agtgttccgc agaacccgcc
     2641 tcaacccgat gctgaggtgg attatccctt ccggctgggg atcgacccgg ctgggttcac
     2701 caacacggat ggcattgccg aatgggcgag ggcaaccggt tccccgatcc gcgtcaatgg
     2761 caatctggtc tggtacggct ccgcgccttc taccacgggc ggcctgccgg agatcacgct
     2821 gaacctgctg gacccgcgcg accagccctg gaacgtggtg gaggtggccg ggaagatcag
     2881 ccgagaaggc cgcaatcagt ggaaacaccc ggatcgcatg gcgctgcggg tctatgaccc
     2941 ctcgcaagcc gggccgggag agctgccgct caactacacc agccagacgc cgctgcccac
     3001 cagcgacgac gatgccgcct tcgcggccgt cctggaaggc tcgcaatcgc cggcgctgac
     3061 agtggcggac tgctatctgg acaacaagtg gctgaccgcg gagcctcaag agaagcaggg
     3121 ggtcgacccg cggcgcagca tccgccggat cgaaccgccc cagattgaga cggtggagcc
     3181 gggcaccggc ctcctgcgct atcaggcgat gacttacggc gcgcccgccg tcactggttc
     3241 cacggcaggg gccagcgttt cgtggcaggc ccgcctggcc gcgcgctatg gctatggcgc
     3301 tggcgtctat attgataacg cccggcacaa ctcgaaccac acccgttgcc agggcgacgt
     3361 tcaattcaac cacagcgccg agaaactgcg cgacgattgg atgcgcccgt actcggagac
     3421 cccgcagggc agcggcagcg ggtggaagca tccgggccgg gtgtatgagc ccgttggcac
     3481 gctgatcatc ctggagccgg acgtcacggt gccgggcggg ccggaaaaac tcggggccgg
     3541 gctgatgagc atcacccggg ccgattaccg cggctggcgc agcccgacag gcgagcgcct
     3601 gccggtgcaa acggtctact ttgagatcca gccgggcact gtcgcctcgg gcgtccagga
     3661 atacaccctc ctgccgaaga tctggagtgg gtcgcaatgg cagacgtggg actggacaag
     3721 ccgcacctgg gtcccctatg atggcacgaa tggcacgcgc taccccttca acggcgtcat
     3781 cttcgccgaa gggaacgtgc ggatccgcgg ccggttgccg cgggataccg agcacggcgg
     3841 gatgcggctt accgtcgtct cgcgcggcac catctacctg gaaggaaacc tgttgaaggg
     3901 ggaccccgcc cctgggatgg ccccccagtc cttcctggcg gtcctggcgc gcgactatgt
     3961 ctgcctgaac acaacgcagt tcgttcaggt gcagaccgat tttgtgccac cgacggaggg
     4021 ctctatcccg ccgttcttct ggcgcctgcc gaataaccgc ccggatctgg agtttcaggc
     4081 gtcctgggac ttcggggttc cgccgaccga tgagtccgtg caaacctact atgggttgag
     4141 tgacccgagt aacccggatt tgaacctctg cccggcgctc ttcttgaggc accaggcgga
     4201 tcgcgacccg gtgagcattc agtggcacct gaataaccac ccttaccggc actacctgta
     4261 cgagcatggc tacgactcga gcgtggctca ggatttctac ccgctcttcc acgttggaga
     4321 gtgggaggtc ttccgcgatc tggtctcgct gatgcccttc ccgctcttcc cggaacccag
     4381 ggcgggttcc gacccgattg cgcatcgcat gcctggcaag cgaaacatca ttcggatcga
     4441 gaactacgat cccgggcagg cgccggatgg cacagcgacg accgggggcg actataacct
     4501 gggggcgatt gccgtgcagc cgctggatat ctggatccag gcgctgatct acgcggagca
     4561 caactcgtgg ttcgtgatcc cgggcgttcc gttccaggat accgacgcgg gcgatccgga
     4621 gccggcaatc tcaccgcgct atccgcgcaa cggtgagccc ctggatgtgc agatccacat
     4681 ttggggcgcc atcgccgagg gccaggccgc gtcgctggaa gacgtgacgc agtggacagc
     4741 gcgctggcgc ggggccaatc gcacggcata cgaggcgtat ggcgatccgg cgctgactgt
     4801 ggatccgaca tttaccaagc gcgggggaat caactacgcc ttcgatccga agctgattac
     4861 cgacacgcgt tgggtgcgct ttgcgcaggg cgagccgatg caccagctcc ccgcgctgcc
     4921 ccgattgccc gtgtgtccgg acctggtcta cttcggcgag cgcccggttc aggagtgagc
     4981 cgccagggac gcgaggcgat tgaggtgggg catcgatcga tatcgatatc cgggccaaga
     5041 cgcgccggcg gcgcaccgcc agcgagccac gccgcaagcc ggtggcggaa gaccgatggc
     5101 tg
//
LOCUS       DAOSCV010000219         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10273_length_5100_cov_3.508226, whole genome shotgun sequence.
ACCESSION   DAOSCV010000219 DAOSCV010000000
VERSION     DAOSCV010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_5100_cov_3.508226"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLY56_06765"
     CDS             complement(<1..463)
                     /locus_tag="PLY56_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21218.1"
                     /translation="MPEQRNLNSLLASLVAAFKVPDLRKRLFFVLQMFAVYMIALHIP
                     LPGIDRAKMDALFRSGGLMSLLDTFSGGALRKYTILAMSITPYINASIIMQLLTVAIP
                     QLEELQKEGEPGRKQISRYTRWLTVILALFQAVAMNLMFKRMGFLDVNALQF"
     gene            complement(483..965)
                     /gene="rplO"
                     /locus_tag="PLY56_06770"
     CDS             complement(483..965)
                     /gene="rplO"
                     /locus_tag="PLY56_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOJ21219.1"
                     /translation="MSEYLSRLRPAAGSVHSRKRVGRGIASGRGKTAGRGTKGQKARE
                     TVRPGFEGGQTPLARRIPHLRGFRPPAKKEYAIVNVAQLAVFDSGAQVTPEALLQYRL
                     ISDLKDGIKILGDGELDRPLVVRAHKFSASAQEKIRQAGGSVEIIPVGRQRGQPEAEA
                     "
     gene            complement(1055..1240)
                     /gene="rpmD"
                     /locus_tag="PLY56_06775"
                     /pseudo
     CDS             complement(1055..1240)
                     /gene="rpmD"
                     /locus_tag="PLY56_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
     gene            complement(1615..2112)
                     /gene="rpsE"
                     /locus_tag="PLY56_06780"
                     /pseudo
     CDS             complement(1615..2112)
                     /gene="rpsE"
                     /locus_tag="PLY56_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(2113..2472)
                     /gene="rplR"
                     /locus_tag="PLY56_06785"
     CDS             complement(2113..2472)
                     /gene="rplR"
                     /locus_tag="PLY56_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ21220.1"
                     /translation="MRIKRTLRERRHLRVRKRVHGTAERPRLGVFRSSKHIYAQLIDD
                     EVGVTLAAASTLDKEFVAEGVSTASKAAAKEVGRLVAKRAVEKGIKRVVFDRGGFLYH
                     GRIASLADGAREGGLEF"
     gene            complement(2551..3108)
                     /gene="rplF"
                     /locus_tag="PLY56_06790"
     CDS             complement(2551..3108)
                     /gene="rplF"
                     /locus_tag="PLY56_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ21221.1"
                     /translation="MSRVGRKPIPVPAGVDVKIADREITVKGPKGTLSHQLPPDLIVT
                     REDGQIVVSRPDDEKIHRCLHGLTRTLIANMIEGVTQGYTKTLEIQGVGYRATKASDT
                     RVTLTLGFAHPVEVIARPGITFDVEGTNRVIVKGIDKQLVGQTAAEIRAVRKPEPYKG
                     KGIRYLGEEVRLKAGKTAKAAKGGK"
     gene            complement(3242..3643)
                     /gene="rpsH"
                     /locus_tag="PLY56_06795"
     CDS             complement(3242..3643)
                     /gene="rpsH"
                     /locus_tag="PLY56_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ21222.1"
                     /translation="MAVTTDPVADLLTRIRNANAANHDQVEVPASKLKQEICRILQDE
                     GYIKSYGVFRVGVHDVIRIQLKYGPRKEKVITQLKRVSKPGLRIYRGKRELPRVLDNL
                     GIAIVSTSQGVMTDREARRRGVGGEVLCYVW"
     gene            complement(3659..3844)
                     /locus_tag="PLY56_06800"
     CDS             complement(3659..3844)
                     /locus_tag="PLY56_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOJ21223.1"
                     /translation="MAKKALIEKAVRPPKFKVQRYYRCRLCGRPRAYMRKFGICRICF
                     REMASRGLIPGITKSSW"
     gene            complement(3934..4476)
                     /gene="rplE"
                     /locus_tag="PLY56_06805"
     CDS             complement(3934..4476)
                     /gene="rplE"
                     /locus_tag="PLY56_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ21224.1"
                     /translation="MARLKERYREQIASALMKEFQYENVMQVPKLVKIVINMGVGQAT
                     QNPKLLDGAVEELAAISGQKPIIRRAKKSIAAFKLRAGMAIGAKVTLRGDRMYEFLDR
                     LVNVALPRIRDFQGVSAQGFDGHGNYSLGLKDQTVFPEIDYSQVERVRGMDITFVTTA
                     SSDDEARALLRHFGMPFRAA"
     gene            complement(4623..4991)
                     /gene="rplX"
                     /locus_tag="PLY56_06810"
     CDS             complement(4623..4991)
                     /gene="rplX"
                     /locus_tag="PLY56_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ21225.1"
                     /translation="MRRQPEKKRGTPKSLFVHKGDQVVVLSGKDVGKRGKVLRALPRE
                     NKVIVEGVNIVKRHQKPRGQVTTKIQAGIIEKPAPIWASKVMVICPGCDEPSRVQHRF
                     LESGEKVRWCRACNSPIDQK"
BASE COUNT          827 a         1808 c         1480 g          985 t
ORIGIN      
        1 caaactggag cgcgttcacg tccaggaagc ccatgcgctt gaacatcagg ttcatcgcca
       61 cggcctggaa gagcgccagg atgaccgtca gccagcgcgt gtatcgagag atctgcttcc
      121 gcccgggctc gccctctttc tgcagctcct ccagctgggg aatagccacc gtgaggagct
      181 gcatgatgat cgaggcgttg atgtagggcg tgatcgacat cgccaggatc gtgtatttgc
      241 gcaacgcgcc tccggagaag gtgtccagca agctcatcag gccgccggag cggaagagcg
      301 cgtccatctt ggcgcggtcg atccccggaa gcggtatatg caacgcgatc atgtataccg
      361 caaacatctg aagcacaaag aacagacgct tgcgcagatc cggtacttta aaggcagcaa
      421 ccagggaagc cagcaacgaa ttcaggttcc gctgctctgg catgggtcct ccttaccgat
      481 ccttacgcct ccgcctcagg ctggccgcgc tgccgtccga cggggataat ctccacggag
      541 ccgccggcct ggcgaatctt ctcctgagcg gaagcactga acttgtgcgc acgcacgacc
      601 agcggccggt ccagttcccc gtcaccgagg atcttgatgc cgtccttgag gtcactgatc
      661 aaccggtact gaaggagcgc ctccggcgtg acctgcgcac cgctatcaaa caccgccaac
      721 tgtgccacgt tgacgatggc atattccttc ttcgcgggcg gccggaaacc gcgcagatgc
      781 gggatccgcc gcgcgagggg cgtctgcccc ccttcaaaac cgggccggac ggtctctcgc
      841 gccttctggc ccttcgtgcc ccgaccggcg gtcttgcccc tccccgaggc aatcccccga
      901 cccacgcgct tgcgtgaatg cacggagccc gcagccggtc ggagtcgcga tagatactcg
      961 ctcattcgtt ggctccttct gtctaggccg cacccgcttc aggtgcggac tgtgccccgg
     1021 gagcaggcgc ctttgcaggc tcctgcgccg gtgccacgct ctccactcgc accaggtgct
     1081 cgatggtgcg gatcatgccc cggatctccg ggcggtctgg gaccacgacg ctcttgttca
     1141 gccgcgtcag ccccagagac cgggcgctgg cgcgttgccg ctcggtattc ccgatgacgc
     1201 tgcgcaccag cgtcactttt atctgctggt caaccaccat actctcccgt tcgtgcgcat
     1261 ccaggcaagt gggccgggct gggtccgcag gctccggcgc cgcggggtcc cggcgtgctt
     1321 gcccttcgcg cccaagcgcg gcggcgtcgg cccgccaggg ccggatcccg gcgctcgctg
     1381 gcgccgggcg caccgccacg acgactactc gccagtcgcc tgcgtctcag cggcctccgc
     1441 agtcggttcg ctcgtctggg cctcggccac ttcctccgca ggaacctgcg ctgcctcggc
     1501 ggtctccgcc tgcgcctcgg gcgtctcagc cgctacggct tcttcctcga cggcctcggc
     1561 ttcggcgccc tcttcctcag ctgccagcac cgagcgcgac tcgcgcagcc agggaaccag
     1621 ctcctcggcg gtcttaccgc gcatccgggc gacctcctcc gcgcgcttca gtcggcgcaa
     1681 gccgtccata gcagcccaca ccgtattgat cgcgttggac gagcccagcg acttgctgag
     1741 gacgtcgctc acgcccgcgg cctcgagcac tgcgcgcacc gcgccgccag caatcacacc
     1801 ggtacccgga gaggccggct tcagcagcac ttccgaggca cccgcgcgtc cgatcaccat
     1861 gtgcgggatc gtggttgcca cccggggaat ggcgatgatg ttcttcttgg cgtcttcgac
     1921 gcccttgcgg atcgcctcgg gaacttcctt tgccttccca agacccgcgc cgacgtgccc
     1981 gttgccatca cccaccacca cgagcacgtt gaagctcggg gtgcgtccgc ccttataggt
     2041 cttcgccacc cggttgatgc gcaccacccg ctcttccagg ttcgatagcg tgttgggatc
     2101 gattcttctc atctagaatt ccagtcctcc ctcacgcgcg ccgtcggcga gtgatgcaat
     2161 ccgtccgtgg taaaggaacc cgccccggtc aaagacgacc cgcttgatgc ccttttcgac
     2221 agcgcgtttg gcgacgaggc ggcccacttc cttggcggcc gccttggaag ccgtggaaac
     2281 cccctcggcc acgaactcct tatcgagcgt cgaggcagca gccagggtca cccccacctc
     2341 gtcatcaatc aactgagcat agatgtgctt gctggagcgg aacacgccca gccgggggcg
     2401 ttccgccgtt ccatgcaccc tcttgcgaac gcgcaagtgg cggcgctcgc gcaaggtcct
     2461 cttgatcctc atcctgttac tcccaccgcg aggtcgctcg ccgcgtcggc cccagaagcg
     2521 gggcgcgcac ctgcgaggag cgcaacccca ctacttgcca cccttggcag ccttggccgt
     2581 cttgcccgcc ttcaggcgca cttcctcgcc aaggtagcga atgcctttgc ccttgtacgg
     2641 ctccggcttg cgcacggcgc ggatctccgc cgccgtctgc ccaaccagct gcttgtcgat
     2701 ccccttgacg atgactcggt tcgtgccttc cacgtcaaag gtaatcccag ggcgcgcaat
     2761 gacctcgacg ggatgcgcga agccgagcgt gagcgtcacg cgcgtatcgg acgccttcgt
     2821 cgcgcggtaa cccacgccct ggatctccag cgtcttggtg tagccctggg tcacgccttc
     2881 gatcatgttg gcgatcagcg tgcgcgtcag gccatggagg caccgatgga tcttctcatc
     2941 gtcgggccgg ctgaccacga tctggccgtc ttcccgggtg acaatcaggt caggcgggag
     3001 ctggtgagag agcgtcccct tggggccctt gaccgtgatc tcgcgatcgg cgatcttcac
     3061 gtccaccccg gcgggcaccg gtatcggttt tctccctact ctcgacattt catatcccca
     3121 atgcagctca gcgcggttcc ccgcgcggcc gcacatcgtc tccaatcacg gaagcaggaa
     3181 cgcgccgccg ctccggctcg gtcgcgtggg cgccctgcct cgcattcgcg ggccgcgcgg
     3241 tttaccagac gtaacagagg acctcgcccc cgacgccgcg gcggcgtgcc tcgcggtcgg
     3301 tcataacgcc ctgggaggtg ctcacgatgg caattcccag gttgtccagc acgcgcggaa
     3361 gctctctctt gccccggtag atccggaggc ccggcttgct cacgcgcttg agctgagtga
     3421 tgaccttctc cttgcgtggc ccgtacttca gttgaatgcg gatcacatcg tggacgccaa
     3481 cgcggaagac gccatagctc ttgatgtagc cctcatcctg aaggatgcgg cagatctcct
     3541 gcttcagctt cgaggccggg acctccacct ggtcgtggtt ggccgcattg gcgtttcgaa
     3601 tgcgcgtcag caggtccgca acgggatcag ttgtcactgc catcttctct cctctgcctt
     3661 accagctcga cttcgtgatc cccgggatca ggccacggga ggccatctcc cggaagcaga
     3721 tgcgacagat cccaaacttg cgcatatacg cgcggggtct cccgcacaga cggcagcggt
     3781 aatagcgttg caccttgaac ttaggcggcc gcacggcttt ctctatgagc gcctttttcg
     3841 ccacgtagtt tccctccttg ctgtgtttcg tagggggcga ttcctcgccg tcgctccggg
     3901 cgccgctacg aacacagtct cacacgggtc aggttaagcc gcgcggaacg gcataccgaa
     3961 gtgccgtagt agcgcgcgcg cctcatcgtc gctgctcgcc gtggtcacga aggtgatgtc
     4021 catgccacga acgcgctcaa cctggctgta atcgatctcg gggaagaccg tctggtcctt
     4081 cagcccgaga gagtagtttc catggccatc aaagccctgg gccgacaccc cctgaaagtc
     4141 tcgaatgcgg gggagggcga cgttcaccag ccggtccaga aactcgtaca tccggtcacc
     4201 gcgaagcgtc accttggcac caatcgccat gccggcgcgc aacttgaacg cggcaatcga
     4261 cttcttggcc cggcggatga tcggcttctg gccggagatg gccgcaagct cctcaacggc
     4321 gccatccaac agtttcgggt tctgggttgc ctgacccaca cccatgttga tcacgatctt
     4381 cacgagcttc ggcacctgca tgacgttctc gtactggaac tccttcatga gggccgacgc
     4441 aatctgctct cgataccgct ctttgagcct tgccaagtat ctaacctccc gagggtcgcg
     4501 cctgcggctc gattcccgac cgagccgctc cctggcggac tgcagaatgg gggcttaatc
     4561 ctcacaccgc tcacgcggcg cctgggcccg acattccaca atctgcgatc agggtgctcc
     4621 ccctacttct ggtcaatggg gctgttgcac gcacggcacc agcgcacttt ctccccactc
     4681 tcaaggaacc ggtgctgcac ccgggacggc tcgtcacatc ccggacagat caccatgacc
     4741 ttcgacgccc aaatcggcgc cggcttctcg atgatgcccg cctgaatctt cgtggtgacc
     4801 tggccgcgcg gcttctgatg gcgcttgacg atgttcacgc cctcgacgat caccttgttc
     4861 tcgcggggca gcgcgcgcaa tacctttccg cgcttgccca cgtctttgcc ggagagcacc
     4921 accacctggt cgcccttatg gacaaagagg ctcttgggcg tccctcgctt cttctcaggt
     4981 tgccttctca tccagtccaa ctcctaccag tccgtgctgc ccgacgggac agcgcggccc
     5041 accatacgcg cgaggctaca gcacctcggg cgccagcgag ataatccgca tgaactcgcg
//
LOCUS       DAOSCV010000220         5095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10294_length_5095_cov_3.255952, whole genome shotgun sequence.
ACCESSION   DAOSCV010000220 DAOSCV010000000
VERSION     DAOSCV010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10294_length_5095_cov_3.255952"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3556)
                     /locus_tag="PLY56_06815"
     CDS             complement(<1..3556)
                     /locus_tag="PLY56_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21226.1"
                     /translation="MILLGIVFLAFGLSAVSAAEAPLLPLDTAEGVRATMTPRGVTSK
                     IEVVTGEGNTTDGKGALHLEGTSPTAQGNKYFGVRIPLKEPVDLRERRILIDARTNFP
                     VETRAFYVRLYNRGEKQPAWSFASWSGQLRKEWRTFSIQVGFSPDGLDWEASVVENRV
                     ASHIDAIELIIGTSEDEKTVDLLCDNIRVAERIQTIQDLKAPKPLVRETVLVRDGKPV
                     ATIIHPESQAGRKAAQAISDAVKAQTGVALPLRAATLADREREAAETQVLLGNVDTNP
                     ALLLPYARYQTPVDSVCPGPGGYLVHTIHDPYGKGTNLVVVGATDDAGLERAAATFAG
                     IIAKQAKGQALVLPRLFERGYSDEFLKRFAWASSKPDPKRLEQGLAEGRRALETGRHT
                     SVAGVLANVANRYRFTGHSVEAKLFVALWDLYGESAVADPRKFGGPWGFDSDFPSVLV
                     VSGWDTIEEDPALTDEERLRVTKIMARWLTEAVIPSVGTSAHHVPHNHQTFPGLGALM
                     AGLYFSNGYNVLEGPRWLAIADNLFRPQAERFKPHEDCNGYQWLTNGHQMRYALARPY
                     FALFTNGSGQKVIDYCIGTMDNLGYQVPYGDTGSWQCWTSELVCLDMFAYATGDPAAT
                     WVANHKRQLRNLMETHAFYRPDKGERPTRFDGVRVWPLEPMYYHSFKAEERPPLERCF
                     DKVSFREALDPKAAYLLLDGLSNGGHKHLDGNSIPRLTQHERIWLADNDYFKAQTKYH
                     NTALVFKEGQASPIPDYIELVGASETKRYGYSRSRASGYSGVDWDRAIVWVKEHKAFL
                     VLDRMTAREVGEYQLRVLWHGVGEVVPAADGMLLRQQGPSLRIQMAPGPRVSIKDDPE
                     LGTNWRGYPYAEPVVRSMTATATVRLKAGESYLFATALHSNPAGDTVPWRMVHLAGTD
                     AVRVSTGKEVFVVGMGPAKALPTADLSTDAHVWAMDQHGVTVLDATRAALGKTTLHAS
                     KARASADQPARVAPQLVRIPSRPAVPNLQAGGQAPAHPVVWAVRPAPERLILSGNMNR
                     PGAVNLGVKLTVDPEPAKANVFSPTGPNRAEALLDGDAQNSTATSVMWDPDQKVTATL
                     DLGAPCLVERVHWAQWWATTSSKKTSYLPEQTVVFLSNDNFQADVRRVGVVTDAGPHP
                     NFGTPINYAVDAGKAPARYVRIEFTPKAGSAIYLSEIVV"
     gene            3836..4213
                     /locus_tag="PLY56_06820"
     CDS             3836..4213
                     /locus_tag="PLY56_06820"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ21227.1"
                     /translation="MPMLESIKPHPSIPLAGAVRVGNMLYVSGQVGYLPGTSEIVPGG
                     VAAQTRQALENIRAILEEAGSSLNHVVKTTVILTDVQRDFAVMNQVYAEVFGDHRPAR
                     TTLGAALARPGLEVEIECIAVIP"
     gene            complement(4331..>5095)
                     /locus_tag="PLY56_06825"
     CDS             complement(4331..>5095)
                     /locus_tag="PLY56_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21228.1"
                     /translation="DEHRHRRRFAIARGQRVEGHLPLLRRSAKLRTAKGETVRLGSDE
                     WVLAREKVGDHFVYGGLKVTLPPGASLRWPAYQHDPYKKDGSSSIGSAKLVVVLPFTE
                     VEEQTVVLSHESPSPFDGLAFEARDLRFTHSEGTYTKRLDDLGSQFIGRTKVGSTLTF
                     TLPEVKPGRYELLGEFVMAHSYGIVRVLLDGKPVGQPFDGYCDGVDGDGERVSFGQVE
                     LGAGPHEVSVELIGKNEKATAMLFSVKRWLLRPIGR"
BASE COUNT          871 a         1693 c         1632 g          899 t
ORIGIN      
        1 ccacaacgat ttcggagagg tagatagccg atcccgcctt cggcgtgaac tcgatccgga
       61 cgtaacgtgc aggcgctttc cctgcgtcca ccgcgtagtt gatcggcgtc ccgaagttgg
      121 gatgcggccc ggcatcggtg acgacgccca cgcggcggac atctgcctgg aagttatcgt
      181 tgctcaggaa gaccaccgtc tgttcgggga gataggatgt cttcttgcta gacgtggtgg
      241 cccaccactg tgcccagtgc acgcgctcca ccaggcaggg tgcgccgaga tcgagcgtgg
      301 ctgtcacctt ctgatccgga tcccacatca cggaggtcgc cgtcgagttc tgggcatcgc
      361 catccaacag cgcctcggcc cggttggggc cggtgggact gaagacgttc gccttggcgg
      421 gctccggatc caccgtcagc ttcacgccca ggttcacggc gccgggtcgg ttcatgttgc
      481 cggagaggat cagccgctcg ggcgccggcc gcacagccca gacgacggga tgcgcggggg
      541 cctgaccacc ggcctgcagg ttgggcacgg ccggccgcga tgggatacgc accaactggg
      601 gagcgaccct ggccggttgg tcggcggatg cccgcgcctt ggacgcgtgc agtgtcgtct
      661 ttccaagcgc ggcgcgcgtg gcatcgagta ccgtgacgcc atgctggtcc atcgcccaga
      721 cgtgcgcgtc cgtgctgaga tcggccgtcg gcagcgcctt cgccgggccc ataccaacga
      781 caaagacctc cttgccggtg gaaactcgca cggcatcggt gccggcgaga tggaccatcc
      841 tccagggcac cgtatcgccc gccggattcg agtggagcgc cgtggcgaag aggtagctct
      901 ctcccgcctt gaggcgcacg gtcgcggtcg cggtcatgct gcggacgacc ggctcggcgt
      961 acgggtagcc acgccagttg gtgccgagct cggggtcatc cttgatcgag acccggggcc
     1021 caggcgccat ctgaatccgc agcgacgggc cctgctggcg caggagcatg ccatccgcgg
     1081 cggggaccac ctcgcccacg ccatgccaaa ggacgcgcag ctgatactcg cccacctccc
     1141 gggccgtcat ccgatcgagc accaggaatg ccttgtgctc tttcacccag acgatcgccc
     1201 ggtcccaatc cacgcccgag tagccagagg cgcgcgaccg gctataccca tagcgcttcg
     1261 tctcgctggc acctaccagc tcgatgtagt cggggatggg cgatgcctgt ccttccttga
     1321 agacgagagc cgtattgtga tacttcgtct gcgccttgaa gtagtcgtta tcggccagcc
     1381 agatccgctc gtgctgggtg agccgcggaa tgctgttgcc atccaggtgc ttgtgcccgc
     1441 cgttgctcag cccatcgagg agcaagtagg ctgccttggg atcgagcgcc tcgcggaagg
     1501 agaccttatc gaagcagcgc tccaggggcg ggcgctcctc ggccttgaag gagtggtaat
     1561 acatcggctc cagaggccag acccggacgc catcaaagcg cgtgggtcgt tcgcccttgt
     1621 caggccggta gaaagcgtgg gtctccatca gattccggag ctggcgcttg tggttcgcta
     1681 cccaggtcgc cgcgggatcc cccgtggcgt aggcgaacat atcgaggcac accaactcgc
     1741 tggtccagca ttgccaggag ccggtatcgc catagggcac ctgatagccg aggttatcca
     1801 tggtgccgat acagtaatcg atcaccttct gcccgctgcc gttggtgaag agggcgaagt
     1861 agggccgggc cagggcatag cgcatctggt gcccgttggt gagccactga tagccgttgc
     1921 agtcctcgtg cggcttgaag cgctctgcct gcgggcggaa aaggttatcc gcgatggcaa
     1981 gccatcgggg tccttccaga acgttatagc cgttagagaa gtagaggccc gccatgagcg
     2041 cccccaggcc ggggaacgtc tggtggttgt gcggtacatg atgggcgctc gtgccgacgc
     2101 tggggataac cgcctcggtc agccagcgcg ccatgatctt ggtgacacgc aggcgctcct
     2161 catccgtgag cgcggggtct tcctcaatgg tatcccagcc ggaaacgacc aggacggagg
     2221 ggaagtcgct atcgaagccc cacgggccgc caaacttgcg cgggtcggcc acggcgctct
     2281 cgccatagag gtcccacagc gctacgaaga gcttcgcctc cacgctgtgg ccggtgaagc
     2341 gataccggtt ggcgacgttg gcaagcacgc cggcgaccga ggtgtgccgg cccgtctcca
     2401 gggcccggcg gccctcggcg agcccctgct ccaatcgctt aggatccggc ttggagctgg
     2461 cccaggcaaa ccgcttcagg aactcatcac tgtagccacg ctcgaagagg cgcggcagga
     2521 caagcgcctg acccttcgcc tgttttgcga taatgccggc gaatgtcgcg gcggcgcgct
     2581 ccaaaccggc atcgtctgtc gcccccacga ccaccaggtt cgtccccttg ccgtaggggt
     2641 catggatggt gtggaccagg tagccgcccg gcccgggaca gacgctatcg accggcgtct
     2701 gataccgcgc gtagggcagg agcagcgccg gattcgtatc cacgttccct aggagcacct
     2761 gcgtctctgc cgcttcgcgc tcgcggtcgg cgagcgtcgc cgcccgaagg gggagcgcta
     2821 ctcccgtttg cgccttcacc gcgtccgaga tggcctgcgc cgccttgcgc ccggcctggc
     2881 tctcagggtg gataatcgtc gccaccggct tcccatcacg caccagcacc gtctcgcgca
     2941 cgagtggctt cggcgctttc agatcctgga tcgtctggat gcgctcggcg acacggatgt
     3001 tatcgcacag gagatcgacg gtcttctcgt cctccgacgt gccgatgatc agctcgatgg
     3061 catcgatgtg actggcgaca cgattctcga caaccgaggc ctcccagtcc aggccatccg
     3121 gactgaagcc cacctggatg gagaaggtgc gccactcttt ccggagctgg ccactccagg
     3181 aagcgaaact ccaagcaggc tgcttctccc cccggttgta gaggcgcacg tagaacgccc
     3241 gggtctccac cgggaagttc gtccgggcat cgatgagaat ccgccgctcg cggagatcga
     3301 ccggctcctt cagcgggatt ctgaccccga agtacttatt cccctgggcg gtgggcgaag
     3361 tcccttccag gtgaagcgcc cccttgccgt ctgtcgtgtt cccctccccc gtgaccacct
     3421 cgatcttcga ggtgactccc cgaggggtca tagtcgcgcg cacgccctcc gctgtatcga
     3481 gaggaagcaa gggcgcctct gccgccgata ccgccgacag cccgaatgcc agaaaaacga
     3541 tacctagaag aatcatgtcg ttctctccat atgagacacc ccgaaacgcc gatgcccgag
     3601 gatcttcatc gcctaatggt tcggcattcg ttcacctccc ccctgcctgc cacaaagctt
     3661 tctgattggt tgccccgcgc cgtcctgatc tcctgccaga gcgcgggtgg gcagagcgaa
     3721 ggaatgcgcg cgcccagggc agtcgatccc tccaccgtgg cagcgaacgg tgagagggag
     3781 cggaaggagg gcacgcgcct ggtcacgaag gggttttacg aaaaggaggc cttgcatgcc
     3841 gatgttggag tcgatcaagc cacacccaag cattccgctg gcgggcgccg tgcgcgttgg
     3901 caacatgctc tatgtctccg ggcaggtggg ttatctaccc ggcacgagcg agatcgtgcc
     3961 cggcggcgtc gccgcgcaga cacgccaggc gctcgagaat attcgcgcca ttctggagga
     4021 ggccggctcg tcgctgaatc atgtcgtgaa gacgacggtg atcctcacgg atgtgcagcg
     4081 cgattttgcc gtgatgaacc aggtctacgc cgaggtgttc ggcgatcacc ggccggcgcg
     4141 caccaccctg ggcgccgctc tcgcccgccc gggcctcgaa gtcgagatcg agtgcatcgc
     4201 ggtcatcccg taatgctcag cccgcacccg ctgacgggac aaggcgcgtc ttccaagggg
     4261 atatgacaag gggtggccac cgcgcccgct actggggcgc cggtaccacc ccttgctgtt
     4321 cggatagaat ctaccggccg atggggcgca gcagccagcg tttcacgctg aacagcatcg
     4381 ccgtcgcctt ctcattcttc ccgatgagct ccacgctcac ctcatgcggg cccgccccga
     4441 gctccacctg gccgaaggag acgcgctcgc cgtcgccatc cactccatcg caatagccat
     4501 cgaaaggctg ccccaccggc ttgccatcga gtagcacccg gacgatccca tagctgtgcg
     4561 ccatgacgaa ctcgccgagc agctcgtagc gcccgggctt cacctctggg agcgtgaagg
     4621 tgagcgtcga gccgaccttc gtccggccga tgaactggga accgaggtca tccaggcgct
     4681 tggtgtaggt gccctcgctg tgcgtgaagc ggaggtcgcg tgcttcgaag gcaagtccat
     4741 cgaatggcga cggtgactcg tgcgacagga cgacggtttg ctcctccact tccgtgaagg
     4801 gaagcaccac caccagcttc gcgctcccaa tggaggagct gccatccttc ttgtaggggt
     4861 catgctggta cgccggccaa cgcagcgatg cccccggggg cagagtcacc ttcaggccgc
     4921 cgtagacgaa gtggtcgccc accttctcgc gcgcgagcac ccactcgtct gagccgagac
     4981 gcaccgtttc gccctttgcc gtgcgcagct tggcagaacg acgaaggaga ggcaggtgcc
     5041 cctcgacgcg ctggccgcgc gctatcgcga agcggcgccg gtgccggtgt tcgtc
//
LOCUS       DAOSCV010000221         5085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10327_length_5085_cov_2.899801, whole genome shotgun sequence.
ACCESSION   DAOSCV010000221 DAOSCV010000000
VERSION     DAOSCV010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_5085_cov_2.899801"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLY56_06830"
     CDS             complement(<1..1181)
                     /locus_tag="PLY56_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase"
                     /protein_id="HOJ21229.1"
                     /translation="MRELEERFADCLVVIGVHSAKFTAESVFANLRQAVLRYDIRHPV
                     LNDAGSIVWQEYAVSAWPTTVVIDPAGRVVGEHVGEFEAGQMAAIVQEIADEYDRQGK
                     LDRTPVAWPLEKEREPARPLSFPGKVLADARSRRLLIADTAHHRILIATLDGGLIAVA
                     GSGSPGLRDGDFTTASFHSPQGIALDGEVLYVADTGNHCIRRIDLQARQVERLAGTGE
                     QARLARRGGPALQTALSSPWDIALRNGTLVIAMAGTHQLWELDLAVGEARRTVGTGRE
                     ALHDDTLQRSALAQPSGLAVANGLIYFADSESSAIRVANLQADHVETLVGEGLFEFGD
                     RDGQGRTVRLQHPLGVAFHDHSLYVADTYNNKIKHIDPARWTTETFAGTGEADHQDGP
                     G"
     gene            complement(1209..1346)
                     /locus_tag="PLY56_06835"
     CDS             complement(1209..1346)
                     /locus_tag="PLY56_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21230.1"
                     /translation="MATRMRGVVRAPEFPAGMEWLNTAAPLSLAGLRGKIVLLDFWTY
                     G"
     gene            1468..2502
                     /locus_tag="PLY56_06840"
     CDS             1468..2502
                     /locus_tag="PLY56_06840"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOJ21231.1"
                     /translation="MKAGLLTAIREVALGEMPDPKPGPGDVLVAVRSVGVCGSDLHYY
                     LRGCIGSQVVEEFPFVLGHEAAGEVVAVGPDVRKLRPGDRVAVEPGIPCGECEPCVTG
                     RYNLCRAVRFLGTPPVHGAYCEYLAVPERYAHPLPPELSYDEGAMIEPLAIGLFASDL
                     AAIQPGYSVAILGAGSIGLVTLMSALAAGAGPVFVSEPLDARREAAKRLGATQVLNPR
                     TDPVVDAILDATDGRGVDVVFEAAGSVEAHQQMVEVVRPAGTIVPIGSCAEELVPLPL
                     ASFRRKGVEVRFVRRFCHTYPRAIGLAQSGKANLSALVTHRFPLEDLAGAFRLLEDYS
                     DGVIKPVVTL"
     gene            2685..3623
                     /locus_tag="PLY56_06845"
     CDS             2685..3623
                     /locus_tag="PLY56_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ21232.1"
                     /translation="MDAARQVAEAVETVILGKSDVVRLAVVCLLSNGHLLIEDIPGVG
                     KTMLARALSRSIGGVFKRAQFTPDLLPADLTGTAIFDQRTGNFEFREGPVFANVLLAD
                     EINRATPKTQSALLECMEERQVSHDGRTYQLPRPFLVIATENVEEYQGTFPLPEAQLD
                     RFLMRLSLGYPDHDNEVAILTRQMKEHPITRLSPVTDPDGIARLQAAVADVHVDESLK
                     DYIVRVVEATRSHPAVHLGASPRGSLGLMRAAQAYAAVEGRNFLIPDDVKALAPAVLC
                     HRLVLRPDARARGATPQEVIDEVLRAVPVPLDAERV"
     gene            3638..4966
                     /locus_tag="PLY56_06850"
     CDS             3638..4966
                     /locus_tag="PLY56_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ21233.1"
                     /translation="MGLYSIWPSLIALVVLAALLVFVLRIRNPKITTLVVAPIFLYLV
                     SAILGSAQLYFMGTMLLGICVVAFLFAHFSLRGILVERTATTPVARGDQVEVRVEVTN
                     RDRLPKLFLRVRCRLPEWLRAEQETVAIAGLWPGQRSVVRVYARALKRGRYALPPVEV
                     VGADPLDLFQRLARFRAPQEVVVHPTPDVAPSLELLGDDLLGTVAPRRVMRPGHGLDF
                     HGLREYRPGDDVRAIDWKATARLSRLITIEFEPTQVGDLVILLDNREKPQIGTGPDST
                     LERAISLAAGAAAAVLEGRGSVHLRAFREGAAFTVSAAAGDLRVLMDALAGVEPEKGD
                     LEALVPEDVRGRTVLLVTPSAGPEVVATARQAAAGGGRAVALLLDRAAWTEERPASGI
                     RRYIQRDAGADGDAETVAAMLHDAGARALVVGRDGLSRERAIRVWEEMAS"
BASE COUNT          736 a         1670 c         1786 g          893 t
ORIGIN      
        1 tctccagggc catcctggtg gtcggcctct cccgtgcccg cgaacgtctc ggtggtccag
       61 cgcgcgggat cgatgtgctt gatcttgttg ttataggtgt cggccacgta gagggagtgg
      121 tcgtggaagg ccaccccgag cgggtgctgc aggcgaaccg tccggccctg gccatcgcgg
      181 tcaccgaatt cgaagaggcc ctcgccgacg agcgtttcga cgtggtcggc ctgcaagttt
      241 gccacgcgga tggcgctgct ctcgctatcc gcgaagtaga ttaggccgtt ggcaacggca
      301 aggccgctcg gctgggcaag ggcgctgcgc tggagtgtgt cgtcatgcag cgcctcgcga
      361 cccgtgccaa cggtccgtcg ggcttcgccc actgccagat cgagctccca gagctggtga
      421 gtgcccgcca tggcgatcac cagcgtgccg ttgcgtaggg cgatatccca cggtgagctg
      481 agcgcggtct gcagcgcggg ccctccgcgt cgggccaggc gagcctgctc cccggtaccg
      541 gcgaggcgct ccacctggcg cgcctgcagg tcgatgcgcc ggatgcagtg gtttcccgta
      601 tcggcgacgt agagtacctc gccatcgagg gctatcccct gtggcgagtg gaagctggcc
      661 gttgtgaagt caccatcgcg caggccgggc gagccggagc cggcaacggc gatcagcccg
      721 ccatcgaggg tggcgataag gatgcggtgg tgcgccgtat cggcgatcag cagccgccgc
      781 gaccgggcat ccgccaacac cttgccgggg aaggagaggg ggcgcgccgg ctctctctct
      841 ttctccagcg gccacgcgac gggcgttcga tccagcttcc cctggcggtc atactcatcg
      901 gcgatctctt gcacgatagc cgccatctgg ccggcctcga actctcccac atgttcgccc
      961 accacgcgcc ccgccgggtc gatcacgacc gtggtgggcc aggcagagac ggcatactct
     1021 tgccagacga tggagccagc atcattgagc accggatggc ggatatcata gcgcagcacc
     1081 gcctggcgca ggttggcgaa aacgctctcg gcggtgaact tggccgaatg cacgccgatc
     1141 accaccaggc aatccgcgaa gcgctcttcg agctccctca actgagggag gacgtggatg
     1201 cagttgattc agccgtaggt ccagaaatca agcaggacga tctttcctcg caggccggcc
     1261 agggagagcg gcgccgccgt gttgagccac tccatgccgg cagggaactc tggggcacgc
     1321 accaccccac gcatcctcgt cgccatgact gcctcctccc gggaggatcc taccccggat
     1381 agtcgtggtg gcaaccccgc ggcggatccg ccaaggagga acacacagcc tgaccggcgt
     1441 aaagtgtgcg cagggagggt tgagcagatg aaggcagggc tgttgacggc gattcgcgag
     1501 gtcgcgttag gcgagatgcc ggatccgaag ccgggtccgg gagatgtgct cgtcgccgtc
     1561 cgctccgtgg gcgtatgcgg ttcggacctg cactactact tgcgggggtg catcggctcg
     1621 caggtggtcg aggagtttcc atttgtcctg ggccatgagg cggcgggcga ggtggtggcc
     1681 gtcgggccgg atgtgcggaa gctgcgccca ggcgaccgcg tcgccgtcga gcccggcatt
     1741 ccgtgtggcg agtgcgagcc atgcgtgacc gggcgctaca acctctgccg cgcggttcgt
     1801 ttcctgggaa ccccgcccgt ccacggcgcc tattgcgagt atctggcggt gccagagcgg
     1861 tatgcccacc cgctgccgcc ggagctctcc tacgacgagg gcgccatgat cgagccgcta
     1921 gctatcggcc tcttcgcgag cgatctggcg gccatccagc cgggctactc cgtggcgatt
     1981 ttgggcgctg gttccatcgg cctggtcacg ctgatgtcgg cgctcgccgc gggagctggc
     2041 cccgtctttg tcagcgagcc gctcgatgcc cggcgcgagg cagcaaagcg cctgggcgcg
     2101 acacaggtcc taaacccgcg tacggatccg gtggtggacg caatcctcga tgccaccgat
     2161 ggccgcggcg tggatgtcgt cttcgaggcg gccggttccg tcgaggcgca ccagcagatg
     2221 gtcgaggtcg tccggccggc gggaaccatc gtgccgattg ggagctgcgc cgaggagcta
     2281 gtgcccctgc ctcttgcgag tttccggcgc aagggggtgg aggtgcgctt cgtgcgccgg
     2341 ttctgccaca cctacccgcg cgccatcggc ctcgcgcaat caggcaaggc gaatctgagc
     2401 gcattggtga cgcatcgctt tccgctcgag gatctggcag gtgccttccg cctgctggag
     2461 gactactcgg acggcgttat caagccggtg gtcacgctct gagactacag ctgtctggca
     2521 gatgggcaga gccgtggcgt agcggctggc atcgcccagt gggcgccttg ttcagcggcg
     2581 attagggatg gtgccgcctg gtcttagccc tccgggagca gcttgaaagg atcgcggtct
     2641 tggcgaacgt cgaaaggagc gccccagcga gtggggcggg tgtgatggat gcggcacgcc
     2701 aggtggctga ggcggtcgag acggttatcc tggggaagtc ggatgtggtg cggcttgccg
     2761 tcgtctgtct tctcagcaac gggcacctgc tgatcgagga tatccccggc gtcgggaaga
     2821 caatgctggc tcgcgcgctc tcgcgctcca tcggtggcgt cttcaagcga gcgcagttca
     2881 cccccgacct tcttccggcg gacctgaccg gcaccgccat cttcgaccag cgcaccggca
     2941 atttcgagtt tcgcgagggc ccggtgttcg ccaacgtcct cctcgccgac gagatcaacc
     3001 gggcgacgcc gaagacgcaa tcggcgcttc tcgagtgcat ggaagagcgc caggtgagcc
     3061 acgacggccg gacgtaccag ctgccacgcc cctttctcgt gatcgctacg gagaacgtgg
     3121 aggagtatca gggcaccttc ccgctcccgg aggcgcagct ggaccgcttc ctgatgcgcc
     3181 tctcgctcgg ctatccagac catgacaacg aggtggcgat tctcacgcgc cagatgaagg
     3241 agcacccgat cacgcgcctc tcgccggtga cagaccccga tgggattgcg cggttgcagg
     3301 cggccgtggc cgatgtgcac gtggatgaat cgctgaaaga ctacatcgtc cgcgtggtgg
     3361 aagcgacgcg ctcgcacccc gcggtgcact tgggggcgag cccgcgcggc tcgctcggct
     3421 tgatgcgcgc ggcgcaggcg tacgccgccg tcgaggggcg caacttcctc atccccgatg
     3481 atgtgaaggc actggctccg gccgtgctgt gccaccgcct ggtcctgcgc ccggacgcgc
     3541 gtgcccgagg cgccacgccg caagaggtca tcgacgaggt gctccgcgcc gtgccggtgc
     3601 cgcttgacgc cgagcgtgtg tagccgggag ccggcggatg ggtctctaca gcatctggcc
     3661 ttccctgatc gcgctcgtcg ttctggccgc cctcctggtc ttcgtcttgc gcattcgcaa
     3721 tccgaagatc accacgcttg tcgtcgcccc tatcttcctg taccttgtct ccgccatcct
     3781 cggatccgcc cagctctact tcatgggcac gatgctcctg gggatctgcg tcgtggcatt
     3841 tctcttcgcc cacttctcgc tccgggggat cctggtagag cgaacggcta cgacgcccgt
     3901 cgctcggggc gaccaggtag aggtgcgcgt ggaggtgacc aaccgcgacc ggctgccgaa
     3961 gctgttcctg cgtgtgcgtt gccggctccc ggaatggctg cgcgcggagc aggagacggt
     4021 cgcgattgcc gggctgtggc cgggccagcg gagcgtggtt cgcgtgtacg cgcgcgcgct
     4081 gaagcgtggg cgctatgctc tcccgcccgt ggaagtggtg ggcgccgacc cgctcgatct
     4141 ctttcagcgc ctggcgcggt ttcgcgcacc gcaagaggtg gtcgttcacc ccactcctga
     4201 cgtcgcgccc agcctggagc tgttgggcga cgaccttctg ggtaccgttg caccccggag
     4261 ggtgatgcgt ccggggcacg gcctcgactt ccacggcctg cgcgagtacc gtccgggcga
     4321 cgacgtgcgc gctatcgatt ggaaggccac cgcgcgcctc tcgcgcctga tcaccatcga
     4381 attcgagcct actcaggtgg gcgacctggt tatcctgctg gataatcggg aaaagccgca
     4441 gatcggcaca ggaccggatt ccaccctgga gcgcgccatc tcgctcgccg caggggcggc
     4501 cgccgcagtg ctggagggcc gcggcagcgt tcacctccgc gccttcaggg aaggcgccgc
     4561 gttcaccgtc tctgcggccg ccggggatct gcgggtgctg atggatgccc tggccggcgt
     4621 cgagcccgag aaaggcgacc tggaggcgct ggtgccggag gacgtgcgcg ggcgcaccgt
     4681 gctcctggtg acgccgtcgg ccggccccga ggtggtcgcc acggcgcgac aggcggctgc
     4741 cggcgggggc cgggccgtag cgctgttgct ggatcgcgct gcgtggaccg aggaacgccc
     4801 tgcctcgggg attcgccgct acatccagcg tgatgcgggg gcggatggtg acgcggagac
     4861 cgtggccgcg atgcttcacg acgcgggcgc gcgcgccctg gtggtgggcc gcgacgggct
     4921 gagccgggag cgtgcgatcc gcgtctggga ggagatggcg tcgtgaggtg gcctggctgg
     4981 ctgacgctgc tcccaggatg gcgcgcgcgc ctgcgcgtgc ttctccccct gtacatcgcc
     5041 acctggctcg cgggttactg cgctcttctg gggctcggct ccgtc
//
LOCUS       DAOSCV010000222         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10354_length_5077_cov_3.086420, whole genome shotgun sequence.
ACCESSION   DAOSCV010000222 DAOSCV010000000
VERSION     DAOSCV010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10354_length_5077_cov_3.086420"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLY56_06855"
     CDS             <1..1218
                     /locus_tag="PLY56_06855"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ21234.1"
                     /translation="GAAKHVAAREGEKPAPRGRLVLKQKEMLQAASPWMHAAYLKPAG
                     TEMINPRGVSTDNGRTWSRIEPTPAFDKGLPHGYRREVAPPFVDPVNGNVLRVVLSMD
                     TPGLDPKIVEPPVALETYYLRYRVSTDGGRSYLFDERIIQKGKTAENPFDGVWVGKNG
                     IFLGDVGSQPIRTREGLILVPAQVCVLGPDGKLASPGGGFTYTDVLILIGRWMEGGRL
                     EWEISERIQADPARTTRGVIEPTLAEMPDGRILCVMRGSNGGRKDPEFALPSYKWYSV
                     STDGGRRWSAPRPWTYEDGTPFYSPSSMSQLLKHSSGRVFWLGNISPTNCRGNSPRWP
                     LVIGEVDPKSLLLKKESVLVLDTKRDDEEGINLSHWWAVEDRETGDIMVAGARHNIGY
                     TARQPYLIRVGVE"
     gene            complement(1284..2339)
                     /locus_tag="PLY56_06860"
     CDS             complement(1284..2339)
                     /locus_tag="PLY56_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008006237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21235.1"
                     /translation="MGKLRVGFIGTGKRKEKRDALGYAMAYDHGAAYQALDYVEMVAC
                     ADIVEENARAFADHFGIPKVYTDYHEMLEKEKLDVVSICTWMHLHEPMVIDCCKAGVR
                     AVYCEKPMANTWGGARRMAEAAKKAGVQLTFNHQRRYGVAWTTAKKLLDEGAIGQLTR
                     LESEAPNIYEFGTHSIDMLSFFNNETPAKWVIAQIDYREEKLVFGGHTENQNLVLVEY
                     ENGVFGFLANGAGGARPVGCFTRLIGTDGVIEVYPVGGPALRYRPAGSKEWIVPELPP
                     EVNMITEAISDALTCLREGRKCQLDVSNALIGTEIIFGAYESSRRRGRVDFPLDIDDN
                     PLEAMVASGDLKPAKPA"
     gene            complement(2484..3641)
                     /locus_tag="PLY56_06865"
     CDS             complement(2484..3641)
                     /locus_tag="PLY56_06865"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ21236.1"
                     /translation="MSNCTAILNATLIDGTGRPPLPESVVVIRSGWFEAVGQAGEVAV
                     PGDVETIDAAGRYLIPGLFDCHMHIGGGPYRAIKTLRDTLAMGVTTVAHVGGFRAGAE
                     VAALRDAIEQGLVGPCSRLVAGAVVAATAGHVMGRTADGPWEVRKAVREQRVANIDFI
                     KTAASGGFWAEHEECWWRDYTYEELEALVDEAHAVGKPVAVHAHTQPGLNNAIRAGAD
                     MIHHGAFIDDEALRGIAERNIFFIPTLRVTSERNIAIKEQAGRPWEARKMREAHPIHR
                     EGVRKARALGIRIGVGTDLPSTPPWRAGQTAVELLELAACGFSPMETIIAATRTSAEC
                     LGKAHELGTIEPGKRADLILLDADPLVALSILTEERHIRMVIKDGEVAIRR"
     gene            complement(3702..>5077)
                     /locus_tag="PLY56_06870"
     CDS             complement(3702..>5077)
                     /locus_tag="PLY56_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ21237.1"
                     /translation="AGGDLSAQGAEWALFHRDPEPPASIPSMALPDRFFPAWKIGYLR
                     SGKDGRRGAAVISASDWGGHHHLDSLNLFYWQDGAELLSDLGYLWDNPDMRNLSRTVA
                     HNLVVVDEANQRTTGRGGWLHAFDISPRVKVIEVSSDAYPQASDYRRTVFLVEHPEGV
                     YLADIFRTSGGKTHDYVFHGPNPEMELQELAADPAPDLTAYQFTQVHATQATRPWRAL
                     WTVDKEVRFQAISLPQPGETSMVAQGWGQRVPQQKGWTLPYIVRRIASDTDATTSAFT
                     SVFEGYRGQAFVRAARRLEVKAPGAVALAVETATGGTDYIVSAPEGTAVTVQTHRGPL
                     VVEGGLAVVATAGEKVRFASLVGGKRLEWNEHRLLLQEPVLRGKVARYENEGPDCWLE
                     LDRALPNPNALVGRTILAGKGEKCTGYEIRAIEGKRIYVRKDGAGVDLLPSEEWRLLL
                     SASLNAE"
BASE COUNT          773 a         1728 c         1697 g          879 t
ORIGIN      
        1 ggagcggcaa agcacgtggc tgcgcgcgag ggggaaaagc cggctcctcg cggtcggctg
       61 gttctgaagc agaaggagat gctccaggcg gcttccccct ggatgcacgc cgcttacctg
      121 aagccggcgg gcacggagat gatcaacccc agaggggtct cgacggataa tgggcgcaca
      181 tggagccgga tcgagcccac tccagcgttc gataagggcc ttccccacgg ctatcgccgg
      241 gaggtcgcgc cgccgttcgt cgatcccgtc aacggcaacg tcctgcgggt tgtcctctcg
      301 atggacaccc ccgggctcga cccgaagatc gtcgagccgc cggtggcgct ggagacctac
      361 tacctgcgct atcgcgtctc cacggatggc gggaggagct acctcttcga cgagcggatc
      421 atccagaagg gaaagacggc ggagaacccc ttcgatggcg tttgggtcgg caagaacggc
      481 atcttccttg gcgatgtcgg ctcgcagccg atccgcacgc gcgagggcct gatcctggtg
      541 ccggcgcagg tgtgcgtcct cggccccgac gggaagctcg cctcgccggg cggaggcttc
      601 acctacaccg acgtgctgat cctgattggc cggtggatgg aggggggccg gctggagtgg
      661 gagatctccg agcggattca ggccgacccg gcgcgcacga cgcgcggcgt gatcgagccg
      721 acgctcgctg agatgccgga cgggcgcatc ctctgcgtga tgcgggggag caacgggggg
      781 cgcaaggacc ccgagtttgc cctgccgagc tacaagtggt attcggtctc taccgacggg
      841 ggccggcggt ggagcgcgcc acgcccgtgg acgtatgagg atggcacccc cttttactcg
      901 ccgagtagca tgtcgcaact cctgaagcac tccagcggac gcgtcttctg gctggggaat
      961 atcagcccca ccaactgccg tgggaacagc cctcgctggc cgctcgtcat cggcgaggtg
     1021 gatccaaaga gcctgctcct gaagaaggag agcgttctgg tcctcgatac gaagcgcgac
     1081 gatgaggagg gcatcaacct ttcgcattgg tgggccgttg aggaccgcga gacgggcgat
     1141 atcatggtgg ccggggcccg gcacaacatc ggctacaccg cccggcagcc gtacctcatt
     1201 cgcgtaggcg tggagtaaac gaaacgcgcc tggcggggaa cccctgcgct ccccgccagg
     1261 cgctgatgcc gtggcaggct gcctcaggcc ggcttggccg gcttgaggtc gccgctggcg
     1321 accatcgctt cgagggggtt atcgtcgata tcgagcggga agtccacgcg tccgcggcgc
     1381 cggctcgact cataggcgcc gaagatgatc tcggtgccaa tgagcgcgtt gcttacgtcg
     1441 agctggcact tgcggccctc gcgaaggcaa gtcagcgcgt ccgagatcgc ctccgtgatc
     1501 atgttgacct ccgggggaag ctcgggcacg atccactcct tcgaccccgc cggccggtag
     1561 cgaagcgcgg gccccccgac gggatagacc tcgatgacgc catccgtgcc gatgaggcgc
     1621 gtgaagcatc ctaccgggcg cgccccgccc gccccgttgg ccaggaagcc gaagacgccg
     1681 ttctcatact ccaccaggac caggttctgg ttctccgtgt gcccgccaaa gactagcttc
     1741 tcctcgcggt agtcgatctg ggcgatcacc cacttcgcgg gcgtctcgtt gttgaagaag
     1801 ctcagcatgt cgatggagtg cgtgccgaac tcgtagatgt tcggggcttc gctctccagg
     1861 cgcgtcagct ggccgatggc gccctcatcc aggagctttt ttgccgtggt ccaggccacg
     1921 ccatagcgcc gctggtggtt gaaggtgagt tgcacccccg ccttcttggc ggcctctgcc
     1981 atccgccgtg cgcctcccca ggtgttggcc atcggcttct cgcagtagac ggcgcgcacg
     2041 cccgccttgc agcaatcgat gaccatcggc tcgtgcaggt gcatccaggt gcagatgctg
     2101 acgacgtcca gcttctcctt ctccagcatc tcgtggtagt cggtgtagac cttcggtatc
     2161 ccgaaatggt cggcaaacgc ccgggcgttc tcttccacga tatcggcgca cgcgaccatc
     2221 tccacgtagt ccagcgcctg atacgcggcg ccgtggtcat acgccatcgc gtagcccagg
     2281 gcatctcgct tctccttgcg cttcccggtg ccgataaacc caacgcgcag tttgcccatc
     2341 gtctctggtc ctcccctgtt tcttcactcg cggtgccccg ctgggagcgc ctgcccaatc
     2401 acttcgctcc tggtgccgct ctccctgccc ccacgcgtgc tcttctggct ttcaggcaac
     2461 cgcccggata cccgcgcggc gcgctaccgg cggatcgcca cctcgccatc ctttatgacc
     2521 atccggatgt gccgctcctc cgtgaggatg ctgagagcca cgagggggtc ggcatcaagc
     2581 agaatgagat cagcacgttt gcccggctcg atggtgccca gttcgtgagc cttgcccagg
     2641 cattcggcgc tggtgcgggt ggcggcgatg atcgtctcca tcggcgagaa gccgcacgcg
     2701 gcgagctcca gcagctcgac ggcggtctgc ccggcgcgcc aggggggcgt gctggggagg
     2761 tcggtgccca cgccgatgcg gattcccagg gcgcgtgcct tgcgcacgcc ctcgcggtgg
     2821 atcgggtgcg cctcgcgcat cttgcgcgcc tcccacgggc gcccggcctg ctccttgatg
     2881 gcgatgttcc gctcggaggt gacgcgcagg gtggggatga aaaagatgtt gcgctcggcg
     2941 atcccccgca gtgcctcgtc atcgatgaac gcaccgtggt ggatcatatc cgcgccggcg
     3001 cgaatcgcgt tgttcagccc gggctgcgtg tgcgcgtgaa cggccaccgg cttgcctacg
     3061 gcgtgcgcct cgtcgaccag ggcttccagc tcctcatagg tgtagtctcg ccaccagcac
     3121 tcctcgtgct cggcccagaa cccgcccgag gcggccgtct tgatgaagtc gatgtttgcc
     3181 acgcgttgct cacgcaccgc cttgcggacc tcccaaggac cgtccgccgt gcgccccatc
     3241 acgtgccccg ccgtggcggc cacgacggcg cccgcgacca ggcgcgagca ggggcccacc
     3301 aggccctgct cgatggcgtc acgcaacgcg gccacctcag cgcccgcgcg gaacccaccc
     3361 acgtgggcta cggtggtcac gcccatcgcc aaggtgtcgc gcagcgtctt gatcgcccgg
     3421 tagggccccc cgccgatgtg catgtggcaa tcgaacagcc ccggaatgag gtagcgcccg
     3481 gcggcgtcga ttgtttccac gtcgcccgga accgccacct cgcccgcctg cccgaccgct
     3541 tcaaaccacc cggatcggat cacgactacg ctctcgggga gcggcggccg gccggtgccg
     3601 tcaatcagtg tcgcgttgag aatcgcggtg cagttgctca cgttcgcccc aatcctcccc
     3661 gcggggggcg atgcgccatg cccgcgggga gctttcagcg ctcactccgc gttcagcgac
     3721 gcggagagaa gcaggcgcca ctcctcggac ggcaggaggt cgacgcctgc gccatccttg
     3781 cgcacgtaga ttctcttccc ttcaatcgcc cggatctcgt aaccggtgca cttctcgcct
     3841 ttgcccgcga ggatggtgcg gccaacgagg gcgttcgggt tgggcagcgc ccggtcgagc
     3901 tccagccagc agtcggggcc ttcgttctcg tagcgcgcca ctttgccgcg cagcaccggt
     3961 tcctgcagca gcagccgatg ctcgttccat tccaggcgtt tgccaccgac cagcgaggcg
     4021 aagcgcacct tttcgccggc ggtcgccacc acggcgaggc caccctctac caccaggggc
     4081 ccacggtgcg tctggacggt aaccgccgtt ccctcggggg ccgagacgat gtagtcggtg
     4141 ccaccggttg ccgtctccac ggcaagcgcg acggctccgg gtgccttgac ctcgaggcgg
     4201 cgcgcggcgc gcacgaacgc ctggccgcgg tagccctcga agaccgaggt gaaggcgctc
     4261 gtcgtggcgt ccgtatcgga cgcgattcgc cggacgatgt aggggagcgt ccaccccttc
     4321 tgttggggca cgcgctggcc ccatccctga gcgaccatcg aggtctcgcc cggctgtggg
     4381 agcgagatcg cctggaaacg aacctccttg tcaacggtcc acagcgcccg ccagggccgg
     4441 gttgcctgcg ttgcgtgcac ctgggtgaac tgatacgccg tcaggtcggg cgccgggtct
     4501 gcggccaact cctgcagctc catctcgggg ttggggccgt ggaagacgta atcgtgcgtc
     4561 ttcccgccgc tggtgcggaa gatatcggcg agatagacgc cctcgggatg ctctaccagg
     4621 aagacggtgc gccggtagtc gctcgcctgc gggtaggcgt cggaggagac ctcgatcacc
     4681 ttcacgcgcg gcgaaatatc gaaggcgtgg agccagccgc cccggccggt ggtgcgctgg
     4741 ttcgcctcgt ccaccaccac caggttgtgc gccaccgtgc ggctgaggtt gcgcatgtcc
     4801 gggttgtccc acaggtagcc gaggtcggag aggagctccg cgccatcctg ccagtagaag
     4861 aggttgaggc tgtcgaggtg gtgatggcct ccccagtcgc tggcgctgat gacggcggcg
     4921 ccgcgccggc cgtccttgcc gctgcgcagg tagccgatct tccaggcggg gaagaagcgg
     4981 tctggcagtg ccatcgatgg aatggatgct gggggctcgg ggtcgcggtg gaagagcgcc
     5041 cactcggcgc cctgagcgga gagatcgcca ccggcca
//
LOCUS       DAOSCV010000223         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10433_length_5046_cov_2.971749, whole genome shotgun sequence.
ACCESSION   DAOSCV010000223 DAOSCV010000000
VERSION     DAOSCV010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10433_length_5046_cov_2.971749"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="PLY56_06875"
     CDS             <1..1563
                     /locus_tag="PLY56_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ21238.1"
                     /translation="EYLFDLLNRNGYEGRVLTVNGTNTDDRSGAIYREWLKRHQGEPV
                     VTGSKAVDLRAALVEQFRDHADILVATEAAAEGVNLQFCSLVVNYDLPWNPQRIEQRV
                     GRCHRYGQAHDVVVVNFLNRENAADQRVFELLSEKFRLFDGVFGSSDEVLGALESGVD
                     FERRIAGIYQSCRTTEEIDAAFNSLQQQLEEEIAARMADTRAKLLEHFDEEVHERLRM
                     SREASRSYLARAERWLWSLTQHELGDVAAFDEERLEFDLHCVPHGWPDAPLGRYQFVT
                     RTTQFDGHHAYRLGHPLAQAAIEHAKERSLPCAQVTFSYTAYAGRVGLVEPLVGRSGY
                     LTLTRLTVSALEDEDHLLFAACDDAGNLLHPEVCERLFHVPGLVSGEAQVPPPVDNTL
                     QQRLHALQEETLARIGQRNARFFDDEVEKLDRWADDLKHGLETELKDLDFQIKQARKE
                     ARLADSLERKLALHRTIKDLETERTRKRRSLYEAQDDIDARKEALISKVEARLHRTTR
                     TENIFTIRWHVG"
     gene            1590..1805
                     /locus_tag="PLY56_06880"
     CDS             1590..1805
                     /locus_tag="PLY56_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21239.1"
                     /translation="MADPERPRTPNEELAAVIAEALAQEGLVPPSRVSEIRGMLASGR
                     AKESDWSLWMGPPPRPKRREGGDDEAR"
     gene            1792..4140
                     /locus_tag="PLY56_06885"
     CDS             1792..4140
                     /locus_tag="PLY56_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ21240.1"
                     /translation="MRRVEKITLKCFRGATKPVELAFDPKRPIALLFGENGTGKSTII
                     DAIDIVCNRQVGSLQGRSSASEREHLPSIGHRTGDVSVSFQFGESTWYATYSGNQIRI
                     AGPDGVPTAHVLRRSQLLRLVEAEPSKRYEQLRRFIDVGAVEKGESSLRDALRTAEAN
                     LNREADRKTDADRQLQGLWQAEGCPGAPAKTALEWAAERGAADDGELELTVKSIEGLV
                     SRIDALDRASDALDVACRAREAAIQVAKDVDEEQAALEATASRDQVDLVEILRRAAEY
                     LGSPRAPNACPVCQRSIEAATLCREIKERLSRMALADELRCKRDNAARGLEQANAMLA
                     NAQAALVRTAVAALAWIDANPVAALQRVPFARGTYRLLTGDAETTAALPQAQDLRAVL
                     SATKPILESDLGIARRDLNLANALRLHHGNIMDANERIQYADALRGALRRALEIVHDE
                     RIRFTQKALDKVVDEWNRLYGLVHPDEPVGSLRLYLDENRKGSIHQDGSFCGRAGVAP
                     QGYFSDAHLDTLGFCLFLALAKAAGAGDRILALDDVFTSVDQAHLARISDVLQAESQH
                     FSHVIITTHLRSWQERYRHGVGPAANVQLISLRRWTPERGVCADKAKLAVEDLADTLA
                     VEPLDRRGVASQAGVLLEFVLDHLTLQYRCSLPRTPEGVYTLGALCDGTAKLAKQLRI
                     ERPAYPETKSGVVEQPEEVLLKPFIDKVSGITFIRNWVGAHYNALGSDISDGEVEGFG
                     KAVLDLLSALVCPVCGDLARRPDGSYWRCECRKTRMFPLAAP"
     gene            4460..>5046
                     /locus_tag="PLY56_06890"
     CDS             4460..>5046
                     /locus_tag="PLY56_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21241.1"
                     /translation="MLKPFHEVRDPIHVFVRFDGIERDVINSRPFQRLRHIHQLALSY
                     LVYPGATHRRFEHSLGVMELASRVFDVVTAREALSDDVLDLLPADAVNDDSRRYWRRV
                     VRMAALCHDMGHLPFSHAAEKELLPAGFTHEHLTRAIITSQEMEQIWGAMTPPLRSVD
                     VVKVALGPREAPDLHFSNWETILAEIIVGSAFGVDR"
BASE COUNT          929 a         1561 c         1709 g          847 t
ORIGIN      
        1 gagtacctct tcgacctgct gaaccgaaac ggctacgaag gccgcgtgct caccgtgaac
       61 ggcacgaata ctgatgaccg ctccggggcc atctaccggg agtggctgaa gaggcaccag
      121 ggggaacccg tcgtcaccgg gtccaaggcg gtcgatctgc gggcggctct ggtcgagcag
      181 ttccgtgacc atgccgacat ccttgtcgcc acagaagctg ccgccgaggg tgtgaacctc
      241 cagttctgct ccctggtggt caactacgac ctgccatgga acccgcagcg gatcgagcag
      301 agggtgggcc gctgtcatcg ctatggccaa gcgcacgatg tggtggtggt gaacttcctg
      361 aaccgcgaga acgctgccga ccagcgcgtc ttcgagctgc tctccgagaa gttccgcctc
      421 ttcgacggcg tgttcggctc atcggacgag gtgctcggtg ctctggagtc cggcgtggat
      481 ttcgagcgcc ggatcgccgg gatctaccag tcctgtcgca ccacggagga gatcgacgcg
      541 gccttcaaca gcctgcagca gcagcttgag gaggagatcg ccgcacggat ggccgacaca
      601 cgggccaagc tgctggaaca ctttgatgag gaagtgcatg agcggctgcg catgagccgt
      661 gaggccagca ggagctatct cgcccgcgca gagcgctggc tctggagcct gacgcagcac
      721 gaacttggtg atgtggcggc gttcgacgaa gagcgtctgg agttcgacct gcattgcgtg
      781 ccgcacggtt ggccggatgc gcccctgggc cggtaccagt ttgtcacgcg gacaacccag
      841 ttcgatgggc accacgccta ccgcctgggc caccccctcg cgcaagcggc tatcgagcac
      901 gccaaggagc gctcgctgcc gtgcgcgcag gtgaccttct cctacaccgc ctacgcaggt
      961 agggtcggct tggtggagcc gcttgtgggc cggtcgggct acctcaccct gactcgcctc
     1021 accgtatccg cccttgagga tgaggaccat ctgctctttg cggcctgtga cgacgcgggg
     1081 aatctgctac atcccgaggt gtgtgagcgc ctgttccacg tgccggggct ggtctccggc
     1141 gaggcacagg tgccgccgcc ggtggacaac acgctgcagc agcgccttca cgccctgcag
     1201 gaggagacac tcgcacggat cggccagagg aacgcccgct tctttgacga cgaggtggag
     1261 aaactggacc ggtgggcgga tgacttgaag catggcctgg aaaccgagct aaaggacctc
     1321 gacttccaga tcaagcaggc acgcaaggaa gcacggttgg ccgactcgct ggagcgcaaa
     1381 ctggcgctcc accgaaccat caaggacctg gaaaccgagc gcacgcggaa gcgtcggtcg
     1441 ctctacgaag cgcaggatga catagacgcc aggaaggaag ccctgatctc gaaggtagaa
     1501 gcacggttgc accggactac acgaacggag aacatcttca cgatccgctg gcacgttggg
     1561 tagtccctgc agtaccccag gaggtgctca tggcagatcc cgagaggccc agaaccccga
     1621 acgaggagtt ggccgcagtc atcgccgagg cgctcgctca ggagggtctc gtgccgccta
     1681 gccgcgtgag tgagatccgc ggtatgctgg catccgggcg cgctaaggag agcgactgga
     1741 gcctatggat gggtccgccc ccgagaccca agagacggga gggcggcgac gatgaggcgc
     1801 gttgagaaga tcaccctgaa gtgcttccgg ggggcgacca agccggtcga gttggcgttc
     1861 gacccgaaaa gacccattgc gctgctcttc ggtgagaacg gcaccggcaa gtctaccatc
     1921 atcgatgcca tcgatatcgt gtgcaatcgg caggtagggt cgctccaagg gcgcagttcg
     1981 gccagcgagc gcgaacatct gccctccatc ggccaccgca ccggggacgt gtcggtttcg
     2041 ttccagttcg gcgagagcac ctggtatgcg acctacagcg gcaaccagat caggattgct
     2101 ggccccgacg gcgtgcccac ggcgcatgtc cttcgccgct cgcaactctt gcggctggtg
     2161 gaggcggagc catcgaagcg ctatgagcag ttgaggcgct tcatcgatgt gggcgcggtc
     2221 gagaagggcg agtcgagcct gcgggacgcc ctccgaacgg cagaggcgaa cctgaaccgt
     2281 gaggccgacc gcaagaccga tgccgaccgg cagttgcagg gcctctggca ggccgagggt
     2341 tgtcctgggg caccagcaaa gacggcgctc gaatgggctg cggaacgagg ggcggctgat
     2401 gacggcgaac tggagctgac cgtcaagagc atcgaaggtc tggtaagccg tattgatgcg
     2461 ctcgaccgcg cgtccgacgc cctcgacgtg gcctgcaggg cgcgggaagc tgccatccag
     2521 gtagccaagg acgtggatga ggagcaggcg gctcttgagg ctacggcgtc gcgtgaccaa
     2581 gtggacctgg tggagatcct ccgcagggcg gcagagtatc tcggctcccc tcgcgcaccg
     2641 aacgcgtgcc cggtctgcca gcgctccatc gaggcggcaa cgctgtgcag ggagatcaaa
     2701 gagaggctat cacgtatggc cttggcggat gagttacggt gcaaacgaga taatgccgcc
     2761 agggggctag agcaggccaa tgccatgctc gcgaatgcgc aggccgcgct cgtgcggacg
     2821 gcagtggcgg cactggcgtg gatcgatgcc aacccggttg cagcattgca gcgcgtgccc
     2881 tttgcgaggg gcacttaccg cctgctgacc ggtgatgcgg agacgaccgc ggccttgccg
     2941 caggcgcagg accttcgcgc cgtgctctcg gcgacaaagc cgatcctgga gagcgacctc
     3001 ggtatcgcgc gcagagacct gaatctggca aatgcccttc ggctgcacca cgggaacata
     3061 atggacgcga acgagcgcat ccagtacgcc gatgccctga ggggtgcgct gaggcgcgcg
     3121 ctggagatcg tccatgacga gaggatccgc ttcacccaga aggcgctgga caaggtggtc
     3181 gatgaatgga accggctcta tggcctcgtt cacccggacg aaccggtcgg tagtctcagg
     3241 ctttaccttg acgagaaccg gaaggggtcc attcaccagg acggctcctt ctgcgggcgt
     3301 gcgggggtcg ctccacaggg ctacttcagc gacgcgcacc tggacaccct ggggttctgc
     3361 ctcttcttgg ccctcgccaa ggccgcggga gcgggtgata ggatcctcgc cctggacgac
     3421 gtgttcacgt ccgtggacca ggcccacctc gcgcggatct cggacgtgct ccaagccgag
     3481 agccaacact tcagccacgt gatcatcacg actcatctcc gctcgtggca ggagcgctac
     3541 cggcacggcg tcggacctgc cgcgaacgtg cagttgatct cgttgcgtcg gtggacgcca
     3601 gaacgcggcg tttgtgccga caaggcgaag ctggcggtgg aggatttggc ggacacgctg
     3661 gcggtggagc cgctggaccg gcgcggggtg gcatcacagg cgggggtgct actggagttc
     3721 gtgctcgacc acctgacgct gcaataccgc tgctccctgc cccgcacgcc agagggcgtc
     3781 tacaccctgg gcgcgctgtg cgacggcact gcgaagctgg cgaagcagct acgcatcgag
     3841 cgccccgcct atcccgaaac gaaatccggc gtcgtcgagc agcccgagga agtgctcctg
     3901 aagccgttca tcgacaaggt gagcgggatc acgttcatcc gcaactgggt cggggcgcac
     3961 tacaacgctc tggggagcga tatctcagat ggcgaggtgg agggcttcgg caaagcggtc
     4021 ctggacctgc tgtcggcgct ggtgtgcccg gtctgtggag acctggcccg ccgcccggat
     4081 ggcagctact ggagatgcga gtgccgaaag acccggatgt tccccctcgc cgctccgtaa
     4141 tcaaacctgg agcgcgaggt gcccttgcgg tgaggcttcg gggtaggcgt gtgcctcgcg
     4201 cctgtgagca atcgccgacg ccacgctatc actggcccag aggtacttgg tagacaggcg
     4261 ggagaagtgc atccgccaaa cccaagagga gatgtttgcg caaggggcga acagatgtat
     4321 actatggtag tggggccacg cattggcggt cgcgtcggca cggcctcgcc gtctggtgtg
     4381 gctgcgccga ccgcccttac ctgcgtagcg ccaaggcgcg gcgttggccg gattctgacc
     4441 agggagcggg tgtaactgga tgctgaagcc attccacgag gtaagggacc cgatccacgt
     4501 cttcgttcgc tttgacggca ttgagcgaga cgtgatcaac tcccgcccgt tccagcgcct
     4561 tcggcatatc caccagttgg ccctgtccta ccttgtctat cctggggcga cccaccgtcg
     4621 cttcgagcac tctctcgggg tcatggaact cgcttcacgc gtgttcgacg tggtgactgc
     4681 gcgagaggcg ttgagcgacg atgtcctgga tctgctgcca gcggatgcgg tgaacgatga
     4741 ctcccgccgc tactggcggc gcgtggtccg catggcggcg ctctgccacg acatgggcca
     4801 tcttcccttc tcgcacgccg ccgagaagga attgctgcct gcgggattca cacacgagca
     4861 cctgacacgg gcgatcatca cctctcagga aatggagcag atctgggggg cgatgacacc
     4921 cccgctgcgc tctgtggacg tggtgaaggt ggccctcggc cctcgcgaag cccccgacct
     4981 ccacttctcg aactgggaga cgatcctcgc cgagatcatc gtggggtcgg ccttcggggt
     5041 agaccg
//
LOCUS       DAOSCV010000224         5040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10456_length_5040_cov_3.399198, whole genome shotgun sequence.
ACCESSION   DAOSCV010000224 DAOSCV010000000
VERSION     DAOSCV010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10456_length_5040_cov_3.399198"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLY56_06895"
     CDS             complement(<1..346)
                     /locus_tag="PLY56_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21242.1"
                     /translation="MNQRILVVDDELGPRESFRMLLKDRFEVHTAASGEECLASIREQ
                     EYALVFLDVRMPGMDGIETLARLRQLRPTLPVVIVTAYTTMEAASRAVEMGAFGSLIK
                     PFTRRDVERYVGL"
     gene            complement(430..2016)
                     /locus_tag="PLY56_06900"
     CDS             complement(430..2016)
                     /locus_tag="PLY56_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ21243.1"
                     /translation="MKLALILTDSPDRVRDLQRNLSGEFSVIVGRSVAESLDLVQTLP
                     VDLALVDLRSPSVRSDDIPERIREWREHCSVVALLPDEGMDGMLAASYDASLRTDSTP
                     YEISAVLDRVMEHQRLLEELGQQREEIRRLRARSERLQAARAPFRPVDKIIKAFSRAL
                     SSGFDQERLLGLFLDTVVEMMHVSKVSVLLHDGNPGEYRVRCYRGLRPDVAEGLRLRS
                     SAGLAAWLAREGCIMTRELADDPGVSAELRKEMDALQAMVSIPMLCGGVLVGILNLNN
                     RVTGSPFQEDELETLFTLASHMAVAVRDMGALQQVRPEKAYTEQVVAAMGAGVITIND
                     REEVVMCNQRAAEILGKAASEVVNHDLRTLPSPLGDLLYEVMCTGNSVRDKEVLLLPA
                     RTPLIVNAYPLAGATGDVMGSALVFNPCPAVSRRDVESLAAAVQQLQTGIGSLAEEMR
                     QSLGEAQAAIQTIPEGPLDPVQVGELRDVLAQELSRLTSLVEKLAATGQDGEGRRERP
                     IVLGPVEQPVESPVSEARRL"
     gene            complement(2021..4213)
                     /locus_tag="PLY56_06905"
     CDS             complement(2021..4213)
                     /locus_tag="PLY56_06905"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21244.1"
                     /translation="MDYPTLLLLLAGTAHLTVGCFVFGRNPKNAVNRAFLVMEVLFAV
                     WALLGAAIYLPELGTDPVVVGRLAHAVALFLWAGFLSLVLVYPEGDTRTPLSLRVLVW
                     VVSGTIAAVAIFTPDLIKEAEVQKRQLRAVYGPLLPAYFGCIGLFLCWILGESLYRRK
                     HSRGKRRVQIDYLLTGLALAGLSPLPSNMVAPLLGIHSMVWVGPCSTLIFTAMVAHAV
                     VHHRLMGIRLVVGRGVANVLALLIAALLFFGLLWLMNVLLVGTGTAKTSIAMLATALL
                     FQPLRNRVQSAVLRYFHRPTYDYQSTVRDASRALTATLDLNAAIDYLLRTVCATLRVE
                     RGLLLLHDRAAGGFRLVGAHSGWAEQVEEEEAPNPGGLARISPLAIILEEVQEPVVRE
                     ELAVRLPGERGRAIERDMEHFGADVAVPLLSGNVLWAILLVGPKISGDVFTSQDIDLL
                     STVGAEAASAVVNAQLHEDAMQADRLATLGGLAAGIAHEVKNPLVAVRTFAELLPEKH
                     DDVEFREQFSQVVVREVDRIDRLIGQLLHYARPQPPRLEPVDLNELIRETLQLLSYEA
                     ARCSVQVETEFDQSLPRAMADASQMRQVVLNIALNAIQAMPGGGILRITTRLLQGRTA
                     APYLLPAEERIEIVFANTGPRIPEAHLARLFEPFFSTRSEGTGLGLSICKRIVTEHHG
                     EIRAGTTEEGLTAFVIHLPAVCPAEIDPEVCLLEEDAGGLVAVGWARG"
     gene            complement(4218..>5040)
                     /locus_tag="PLY56_06910"
     CDS             complement(4218..>5040)
                     /locus_tag="PLY56_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOJ21245.1"
                     /translation="ISIACRCEGPTSTISAGCASGTAAIGAAREEIRSGRADVMLSGG
                     ADGAVTPYVLAAMCATRVLPSQKDGEDPGEISRPFDATRRGGVLAEGAGVLVLEELNH
                     ALRRGARIYGEIVGYGSNAEAHGMVGFADNDTFLERSMRLALADGRLRPNEVDYICAH
                     GPSDGCDTQETRAIKAVFGAHAYSLAVSSIKSMIGNPLASAGPLQVITAALALEHQKV
                     PPTTNYQVPDPECDLDYVPNQPRVCRVNVALVNSHGFGGANHSLVVARYRDYRRG"
BASE COUNT          959 a         1734 c         1545 g          802 t
ORIGIN      
        1 ccaggccaac atagcgctcg acatcgcgac gggtgaatgg cttgatcaac gagccgaagg
       61 cacccatctc aaccgcgcga ctggcagcct ccatcgtcgt gtaggccgtg acgatgacga
      121 ccgggagcgt gggccgaagc tggcgcaacc tggccagcgt ctcgatccca tccatcccgg
      181 gcatgcgcac atccaggaac accagagcat actcctgctc ccggatgctt gccagacact
      241 cctcgccgct cgcggcggta tgcacctcga accggtcctt gagaagcatc cggaacgact
      301 cgcgcgggcc cagctcgtca tcgactacca gaatgcgctg attcatgccc ttcacactct
      361 cctgccgcac acccccgaac catattcggc ggcaacttca cggctccttg ctcaggaaac
      421 ctgctccgct cacaaccggc gtgcctcact gaccgggctc tccaccggct gctcaaccgg
      481 ccccagcaca atcggcctct ctctccgccc ctcgccgtct tggccggtcg ccgcgagctt
      541 ctccaccaag ctggtcaggc ggctcaactc ctgtgccagg acgtctcgca gctcgcccac
      601 ctgcaccgga tccagtggcc cctccggaat ggtctgaatg gctgcctgcg cctcaccgag
      661 cgattggcgc atctcttctg ccagtgaccc gatgccggtc tgcagctgct gcacggctgc
      721 ggcgaggctc tcaacatccc gccgagagac agccgggcac ggattgaaca ccaaggcgct
      781 tcccataaca tcgccggtcg caccggccag gggatacgca ttcacgatca agggggtgcg
      841 cgctggcagg aggagcacct ccttatccct caccgaattg cctgtgcaca tcacctcata
      901 cagcaagtca cccagtggag agggcaaggt gcggaggtcg tgattcacca cttccgaagc
      961 ggctttcccc agaatctcgg ccgcgcgttg gttgcacatc accacctcct cgcggtcatt
     1021 gatggtgatc acgcccgcgc ccatggcggc caccacctgc tcggtgtagg ccttctcagg
     1081 gcgcacctgt tgcagggcgc ccatatcgcg cacggcgaca gccatgtggc tggcgagggt
     1141 gaaaagcgtc tccagctcat cctcctggaa tggcgacccg gtgacgcggt tgttgaggtt
     1201 gaggatcccc accagcacgc cgccgcacag catcgggatg ctcaccatcg cctgcagcgc
     1261 atccatctcc ttgcggagct ctgcagagac tcctgggtcg tcggccagct cgcgggtcat
     1321 gatgcagccc tcgcgcgcca gccacgcggc aagcccagca gaagagcgaa gccgcagccc
     1381 ctccgccacg tcggggcgaa gcccgcggta gcagcgcacg cggtactctc ccggatttcc
     1441 atcgtggagg agcaccgaga ccttgctgac atgcatcatc tcgaccacgg tatcgaggaa
     1501 gaggccaagg aggcgctcct ggtcgaagcc ggatgagaga gcgcgcgaaa acgccttgat
     1561 gatcttgtcc accggccgga agggcgcacg tgctgcctgg aggcgctcgc tccgtgcccg
     1621 cagacgccgg atctcctcgc gttgctgccc cagctcctcc agaaggcgct ggtgctccat
     1681 cacccggtcg agaaccgcgc tgatctcata cggggtcgag tccgttcgga gggaggcatc
     1741 gtacgaggcc gccagcatgc catccatccc ctcatccggc aacagcgcga ccacggagca
     1801 gtgctcgcgc cactcacgga tgcgctctgg gatgtcgtcg gagcgaacac tcgggctccg
     1861 gagatcgacc agcgccagat ccaccggtag ggtctgcacc aggtccaggc tctccgccac
     1921 cgaacgcccg acgatgacgg agaactcgcc agaaaggttg cgctgcagat cgcgcacgcg
     1981 gtcaggagaa tcggtcagga tgagtgccaa cttcatagta ctaccctcgc gcccacccca
     2041 ccgctaccag gccccccgca tcctcttcga gcaggcagac ttctggatcg atctcggcgg
     2101 ggcacacggc cggcaagtgg atgacaaatg cggtcaggcc ctcttcggtc gtccctgcgc
     2161 ggatttcgcc gtggtgctcc gtcacaatcc gcttgcagat ggaaagcccc aggccggtcc
     2221 cctcacttcg ggttgagaag aagggttcga agagtctcgc cagatgggcc tccgggatgc
     2281 gcgggcccgt gttggcaaag acgatctcga ttcgctcctc ggctggcagg agatagggag
     2341 cggccgtgcg cccctgcaac agccgcgtgg tgatgcgcag aatgccaccc ccaggcatcg
     2401 cctgaattgc attcaacgcg atattgagga ctacctgccg catttgcgag gcgtcggcca
     2461 tcgcccgtgg cagggactga tcgaactctg tctccacctg aaccgagcag cgtgccgctt
     2521 cgtaggaaag gagctggagc gtctcccgga tcagctcgtt cagatcgacg ggctcgagcc
     2581 gcggcggctg ggggcgcgca taatgaagca gttgcccgat caaccggtcg atccgatcca
     2641 cttccctcac aacgacctgg gagaactgct cgcggaactc gacatcgtcg tgcttctccg
     2701 gcaggagctc cgcgaacgtt ctgactgcga ccaacgggtt cttgacctcg tgcgcaatcc
     2761 cggcggccaa tccaccgagg gtcgccagcc ggtcggcttg catcgcgtcc tcatgaagct
     2821 gcgcgttcac aacagcgctt gcagcctccg cccccacggt ggagaggagg tcgatatcct
     2881 gggaggtgaa gacatcaccc gagatcttgg ggccaacgag gagaatcgcc cacagcacgt
     2941 ttccagagag gagcggcacc gccacatccg cgccaaagtg ctccatgtcc ctctcaatcg
     3001 cccggcccct ctccccgggc aggcgcacgg caagctcctc acgcacgacc ggctcctgga
     3061 cttcctccag gataatcgca agggggctga ttcgggcgag cccccctgga ttaggcgctt
     3121 cctcttcctc tacctgctct gcccagccac tgtgcgcacc caccaaccgg aagcccccgg
     3181 cggcgcggtc gtgcaggagc agcagcccgc gctccacacg aagagtcgcg cagacggtgc
     3241 gcagcaggta atcgatcgcg gcgttcagat ccagcgttgc ggtcaaggca cggctggcat
     3301 cacgcaccgt actctgatag tcgtaggtgg gccggtggaa gtagcgcaac accgcgctct
     3361 gaactcgatt gcgtagtggc tggaagagca acgccgttgc cagcatagcg atgctggtct
     3421 tggcggtgcc cgttcccacc agaaggacgt tcatcagcca gaggaggccg aagaagagca
     3481 acgccgcgat caggagcgcc agcacgttgg caacgccgcg cccaaccacc agccggatcc
     3541 ccatcagccg gtgatgcacc acggcatggg ccaccatggc ggtgaagatg agcgtggaac
     3601 agggccctac ccacaccatc gagtgaatac cgaggagcgg ggcgaccatg ttcgatggca
     3661 gcgggctcag cccggccagg gccagtccgg tcagaagata gtcgatctgc acccggcgct
     3721 tgccgcgcga gtgcttccgg cggtagaggc tctcgccgag gatccagcag aggaagaggc
     3781 cgatgcaccc gaagtaggcc ggcagcagcg gcccgtaaac ggcgcgcaac tgcctcttct
     3841 gcacctcggc ttccttgatc aggtcgggcg tgaagatggc gaccgccgcg atcgtgccac
     3901 tgaccaccca caccagcacg cggagagaga gcggtgttcg ggtatctccc tcggggtaga
     3961 cgagcacgag cgagagaaac cctgcccaaa ggaagagcgc cacggcatgg gccaaccgcc
     4021 ccaccaccac gggatccgtc cccagctccg ggaggtagat cgcggcgccc aggagtgccc
     4081 agacggcaaa cagcacctcc atcacgagga aggcacggtt caccgcgttc ttcgggttgc
     4141 gcccgaagac gaagcacccg acggtgaggt gcgccgtgcc cgccaggagc aggagaagag
     4201 tggggtagtc catggggtca cccccgcctg tagtcgcggt agcgagctac cacgagcgaa
     4261 tggtttgcgc ccccgaaacc atgcgaattc accagcgcca cattcacccg acacacccgg
     4321 ggctggttgg gaacgtaatc gaggtcgcat tcggggtcag ggacctggta gttggttgta
     4381 ggcggcacct tctggtgctc cagggccaac gccgcggtga tcacttgcag gggccctgcc
     4441 gaagccagcg gattgccgat catcgacttg atagagctaa ccgccaggct ataagcgtgc
     4501 gccccgaaca ccgccttgat agcgcgcgtc tcctgcgtat cgcagccgtc ggagggcccg
     4561 tgggcgcaga tataatcgac ctcattcggg cgcagccggc catccgcgag ggcaaggcgc
     4621 atactgcgct ccagaaaggt gtcgttgtcc gcaaatccca ccatgccgtg ggcctcggcg
     4681 ttggagccgt agcccacaat ctcgccatag atccgggcac cgcgacgaag cgcgtggttc
     4741 agctcctcca ggacgaggac tcccgcgccc tcggcgagaa cgcccccgcg gcgagtggca
     4801 tcgaacggcc gagagatttc gccgggatcc tcgccatcct tctgggatgg aagtacccgg
     4861 gtggcgcaca tcgcggccaa gacatagggc gtcaccgcgc catcggcccc gcccgacagc
     4921 atcacatcgg ctcgtccgga gcggatctcc tcgcgggccg ccccaattgc cgcagtgcca
     4981 gatgcacacc cggcggagat cgtgctggtg gggccctcac accggcaggc gatggagatt
//
LOCUS       DAOSCV010000225         5039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10459_length_5039_cov_3.535514, whole genome shotgun sequence.
ACCESSION   DAOSCV010000225 DAOSCV010000000
VERSION     DAOSCV010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10459_length_5039_cov_3.535514"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLY56_06915"
     CDS             complement(<1..663)
                     /locus_tag="PLY56_06915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOJ21246.1"
                     /translation="MIVTVTLNPAVDRTLWVRSLAPGDTNRVLRSVMDPGGKGINMSL
                     VLRECGVQTTALGFIAGHTGQFIKTYLENRGIATSFVEVKGETRINIAVEDAGGGPPT
                     TLHEPGPEVGMEDLRQLWDRIRPLLKQAQFIAFGGSLPPGVPNDVYATLIRNCQDNGV
                     RAILDADGTPLMAGLNAKPYLVKPNRAEAERLLGRSIRSIEDAASGALELVQRGVSFA
                     VIS"
     gene            complement(735..1310)
                     /gene="raiA"
                     /locus_tag="PLY56_06920"
     CDS             complement(735..1310)
                     /gene="raiA"
                     /locus_tag="PLY56_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ21247.1"
                     /translation="MRITVKGKRMEVTDGLKSYAEKKLRKLEKYFHHIQEVQITQSVE
                     RQWHVVEVLVQGDGILLRGEERTSDMYASIDQVLEKLERQLQKFKGKRILLPREQTAQ
                     MKERLGDAAAEELVEEVPAEPEPGESPLDSIRVVRTKRFAVKPMSPEEAAMQMELLGH
                     DFFVFANAETEQINVVYRRRGGDYGLIEPEV"
     gene            complement(1325..2524)
                     /gene="ispD"
                     /locus_tag="PLY56_06925"
     CDS             complement(1325..2524)
                     /gene="ispD"
                     /locus_tag="PLY56_06925"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ21248.1"
                     /translation="MGATCAAVVPAAGRGERVGSPCNKLLLPLAGKPVLARTLAALDA
                     CPEIAAIILAVREQEREAICALVRAHGIRKVREVVAGGASRQESVYHGVQAVPDEIPL
                     VAVHDGARPLILPSQITACIEEAARVGAALLALPVTDTLKDVDPEGQVRRTLDRSCVW
                     RAQTPQVFRRDWLLDAHRRAAAEGYQATDCCDLLERLGYPVKVVPGSPENIKITSPQD
                     FAIAEGILRERSGQKPGMAGVRVGFGYDAHRLAPGRALVLGGVRIPYERGLEGHSDAD
                     VLLHAVTDALLGAIAAGDIGRLFPPTDPAYKDADSRLFLRRAGECVRERGMRILCVDA
                     TLMAEAPKIAPYVPQMRAAIAETLQIELDRVSVKATTTEGMGFTGRGEGMAAQAVATV
                     CESEAGG"
     gene            complement(2454..3632)
                     /locus_tag="PLY56_06930"
     CDS             complement(2454..3632)
                     /locus_tag="PLY56_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOJ21249.1"
                     /translation="MVTRYLSWAFLILFSIIGGILGKAAGDIYFRIGAVRRLMGTSEL
                     YSITATIAFIALGVIMTSYLSQVINRQVMLLSNNLEKLSTAEKLEVLIGVVVGITVAT
                     IMVYPWPLSATIKVCVWMVAGTAFAYLGARLFLSMKEDIFLILPSGSGLAKAPAQQDQ
                     GEKPRERIKILDTNVIIDGRISDVCRTGFVEGPIYVPGCVLEELHHIADSTEPLKRAR
                     GRRGLDILQQMQKDMHLLVRTYDHLPLEGNGEVDYQLVQLAKKLDGAIITNDFNLNKV
                     AELEGVTVLNVNELANALKPVVLPGEEMSVTLIREGKEINQGVAYLEDGTMVVVEGGR
                     RHIGETLDVTVTSVLQTVAGKMIFAEVKSANGGEEGGSNMRSGSAGGGQRRKSRQSVQ
                     "
     gene            complement(4002..>5039)
                     /gene="radA"
                     /locus_tag="PLY56_06935"
     CDS             complement(4002..>5039)
                     /gene="radA"
                     /locus_tag="PLY56_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ21250.1"
                     /translation="RVLYVSGEESTQQIKLRADRLSVAADELFLLSETDIDLIEGQVA
                     ASQPQFLIIDSIQTVYTPGIESAPGTVSQVRECTARLMRLAKATHLPVFLVGHVTKEG
                     NLAGPRVLEHMVDTVLYFEGDRHQAFKVLRAVKNRFGSTDELGIFEMRDSGLTEIANP
                     SEIFLSERPMQVPGSVVIAALEGTRPLLVEVQALASPAYFGTPRRVAAGIDYNRMCLI
                     VAVLEKRLGLGLGARDVYLSIAGGVRVTEPAADLGVAVSIASSFFDAPVDPTTVFIGE
                     VGLAGEVRAVQQIDRRLNEAARLGFTRAIVAERQAVKSGAPRGGLRVVGVETAHQAVD
                     LVLGNGQETHE"
BASE COUNT          871 a         1678 c         1552 g          938 t
ORIGIN      
        1 cgagatgacc gcaaagctca ccccgcgctg aacaagctcc agggctccgg acgcggcatc
       61 ctcgatagag cggatcgacc ttccgagaag gcgctctgcc tcggcgcgat tcggcttcac
      121 cagatacggc ttcgcattca aacccgccat gaggggcgtg ccgtcggcat cgaggatggc
      181 gcgcacgcca ttgtcctggc aattgcggat cagcgtggca tagacgtcgt tgggcactcc
      241 gggaggcagg ctcccgccga aggcgatgaa ctgggcctgt ttgaggaggg gccggatccg
      301 gtcccacaac tggcgcaggt cctccatgcc tacttccggc cccggctcat ggagcgtcgt
      361 gggggggccg ccgcccgcgt cctccaccgc gatgttgatc cgtgtctcgc ccttcacctc
      421 cacgaaggag gtcgcgatgc ctcggttctc caggtaggtc ttgatgaact gacccgtatg
      481 cccggcaatg aagccgaggg cagtggtctg gacgccgcac tcgcggagca cgagcgacat
      541 gttgatccct ttgccgcccg gatccatcac actacgcagc acgcggttcg tatcgccggg
      601 cgccagactg cgcacccaca gcgtgcggtc caccgccggg ttcagggtga cagtgacgat
      661 catccgctta taccattctc atcgggacag gacgcccttg cgcgccttag ccgtcatgat
      721 gagccaagag ggggctacac ctcgggttcg atcaacccgt agtcgccgcc tcggcgccgg
      781 tagaccacgt tgatctgctc ggtctcggcg ttggcgaaga cgaagaagtc gtgaccaagc
      841 agctccatct gcatcgctgc ttcttctgga gacatcggct tgactgcgaa gcgcttggtg
      901 cgcacgacgc ggatgctgtc gagagggctc tcgcccggtt cgggctctgc cggaacctcc
      961 tcgaccagct cttccgcggc cgcgtctccc aggcgctcct tcatctgggc tgtctgctcg
     1021 cgcggaagca gaatccgctt ccccttgaac ttctggagct ggcgctccaa cttctcaaga
     1081 acctggtcga tggaggcgta catgtccgac gtgcgctctt cacccctcag gaggatgccg
     1141 tccccctgga cgaggacctc gaccacgtgc cactggcgct ccacactctg cgtgatctgc
     1201 acctcctgga tgtgatggaa gtacttttcg agcttacgca acttcttctc agcgtagctc
     1261 ttcagcccat ccgtcacttc catgcgcttg cctttgaccg tgattcgcat cgcacactcc
     1321 tcctctatcc gccagcctcc gactcacaca ctgtggcaac agcctgtgct gccatgccct
     1381 cgcctctgcc ggtgaagccc atgccttcgg tggtggtggc cttcacgctc acccggtcga
     1441 gctcaatctg cagcgtctcc gcgatagcgg ctcgcatctg gggcacgtag ggggcgatct
     1501 ttggggcctc ggccatcagc gtggcatcga cgcagaggat gcgcatgcca cgctcgcgca
     1561 cgcactcgcc tgcccggcgc aggaagaggc ggctatcggc gtctttatac gcggggtcgg
     1621 tgggtgggaa cagccggccg atatcgccgg ccgcgatagc gccgagcagc gcatcagtca
     1681 cggcgtggag cagcacatcg gcgtccgaat gcccctccag cccccgctca tacgggatgc
     1741 gcacgcctcc cagcaccagc gcccgccctg gcgccaggcg atgcgcatcg tatccgaacc
     1801 ccacgcgtac gcctgccatg cccggcttct gcccgctcct ttcgcgtaag atcccttccg
     1861 cgatcgcgaa gtcctgcggt gatgtgattt tgatgttctc cggtgaaccc ggcacaacct
     1921 tcacgggata gcccaggcgc tccaggagat cgcagcaatc cgtggcctga tagccttccg
     1981 ccgctgcgcg ccggtgggca tccaacagcc aatcgcggcg aaacacctga ggcgtttgag
     2041 ccctccacac gcagcttcgg tcgagtgtgc ggcgtacctg gccctccggg tcgacatcct
     2101 tcagggtatc ggtgaccggg agggcaagca gggcagcccc aacgcgcgcg gcttcctcga
     2161 tacacgccgt gatctgactg ggcaggatga ggggacgcgc gccatcgtgg acggcgacga
     2221 gggggatctc gtctggaacc gcctgcacgc catggtacac cgactcttgc cgcgaggcgc
     2281 cgccggccac tacctcgcgc accttgcgta tcccgtgggc gcgcaccaag gcgcagatcg
     2341 cctcgcgctc ttgttcgcgc acggccaaga tgatggcggc gatttcagga catgcgtcca
     2401 gggcagccag cgtgcgcgca agaaccggtt tcccggcgag cggcagtagc agcttattgc
     2461 acggactgcc gactctttcg cctctgcccg ccgccggcac taccgctgcg catgttgctc
     2521 ccaccctctt cgcctccatt tgccgacttg acctcggcga aaatcatctt gccagctacc
     2581 gtctggagca cgctcgtgac agtgacatcg agcgtctcgc cgatgtggcg gcggccgccc
     2641 tcaaccacga ccatggtacc atcttcgaga taagcgacac cctggttaat ctctttccct
     2701 tcgcgaatca gggtgacact catctcctcg cccggcagca ccacgggctt cagcgcgttc
     2761 gcgagttcat tgacgttgag gacagtaacc ccctccagct cggccacctt gttgaggttg
     2821 aagtcgttgg tgatgatggc cccatccagc ttcttggcca gctgcaccaa ttggtagtcc
     2881 acctcgccat tcccctccag ggggagatgg tcgtaggtac ggacaaggag gtgcatatct
     2941 ttctgcatct gctggaggat atccaggccg cggcgcccac gcgcgcgctt gagcggctcc
     3001 gtggagtcgg cgatatggtg tagctcctcg aggacgcagc ccggaacgta gatagggccc
     3061 tcgacgaagc cggtgcggca cacatccgag atccggccat cgatgatcac gtttgtatcc
     3121 aggatcttga tccgctctcg cggcttttca ccctggtcct gctgtgccgg tgccttggcc
     3181 agcccgctcc ctgaaggcaa gatcaggaag atatcctcct tcatgctgag gaacagacgg
     3241 gcgcccaggt aggcgaacgc ggtgcccgcg accatccaca cgcacacctt gatcgttgcc
     3301 gacaacggcc agggatagac catgatcgtg gccaccgtga tgcccaccac gacgccgatg
     3361 agtacctcca gcttctcggc ggtggaaagc ttctcgaggt tgttgctcaa cagcatcacc
     3421 tggcggttga tcacctggga gaggtagctg gtcatgatga cgcccagggc tatgaatgca
     3481 atcgttgccg tgatggagta gagctcggag gtgcccatca gccgcctgac cgctccgatc
     3541 cggaagtaga tatcacccgc ggctttgcca aggatccccc cgatgatgct gaaaagaatc
     3601 aggaaggccc aactcaggta gcgcgttacc actcggtgtc acccctcttt ctacgccata
     3661 tcccagggaa cgctggccca gtcacacgcc ctcgcgcgat ctggtcttgc ggttcaggcg
     3721 gtcgtccggg ggatagtggc cgggtcgtcg ctatccgggg ggaagaaacc cggtatcagc
     3781 gagtggaccc gttcccggtt gcgtctgatg gcgagcaggc agaatagacg gcatgggatc
     3841 ctctgcccga gacgcactct tactctatat gtatgcgccg gatcctcccg agtccttctt
     3901 cgcgagcagg caggcactgc cccttcccag gatgcgccgg cgggttgcga cggctagaaa
     3961 gcgcggtcgg ctggatgccc cgggcaagcc aggatgcgag gctattcatg ggtctcctgg
     4021 ccattcccca gtaccaggtc gacggcctga tgggccgttt ccacgcccac cacacggagc
     4081 ccgcctcggg gggcgccgga cttcaccgcc tgacgttcgg cgacgatggc gcgcgtgaat
     4141 ccaaggcgcg cggcctcgtt tagccttcgg tcgatctgct gcacggcgcg cacctccccg
     4201 gcgagaccca cttcaccgat gaagacggtg gtcggatcca ctggggcatc gaagaaactc
     4261 gacgcgatgg agacggccac gccgagatcc gccgccggct cggtgacgcg gactccgccg
     4321 gcgatgctca ggtagacgtc gcgagctccc agacccagcc cgaggcgctt ttcgagcacg
     4381 gcgacgatca gacacatgcg gttgtaatca atgccggcgg caacgcggcg cggcgtgccg
     4441 aagtaggcgg gagatgcgag cgcctgcacc tccaccagga gtggccgggt gccttccagg
     4501 gcggcgatga cgacggatcc cggcacctgc atcggtcgct ccgagaggaa gatctcggag
     4561 gggttggcga tctcggtcaa tccgctgtcg cgcatctcga agatgcccag ctcgtcggtg
     4621 gagccaaagc ggttttttac ggcgcgcagc accttgaacg cctggtgccg gtcgccttcg
     4681 aagtagagga cggtatcgac catatgctcc aggacgcgcg ggcctgccag gttgccctct
     4741 ttggtgacgt gccctaccag gaagaccggc aggtgagtcg ccttcgcgag gcgcatcagc
     4801 cgggcggtgc actcacgcac ctggctcacc gtgcctgggg cgctctctat gcccggcgtg
     4861 tacaccgtct ggatggaatc gatgatgaga aactgcggtt gggaagccgc aacctggccc
     4921 tcgatgaggt cgatgtcggt ctccgagagc aggaacaact cgtctgcggc caccgagagc
     4981 cggtcggcac gcagcttgat ctgctgagtc gactcctcgc cggagacgta gaggacgcg
//
LOCUS       DAOSCV010000226         5032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10487_length_5032_cov_3.193691, whole genome shotgun sequence.
ACCESSION   DAOSCV010000226 DAOSCV010000000
VERSION     DAOSCV010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10487_length_5032_cov_3.193691"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLY56_06940"
     CDS             complement(<1..532)
                     /locus_tag="PLY56_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21251.1"
                     /translation="MILTTLFGAGADAAPAQGGPITLEAEAAQLDPNRTEIVAQESFA
                     SKRGVALKAGVASNVGKPDTAPDLVFRVRAPQAGRYWIRTHAATDAHGTELMRRATGK
                     QASLRLMISVDGSRPTSRVVFVPWSRPESCKQATGKFDFNGQEQEIRVWLPAGVRLDY
                     LQVSPYVPPRVPAQVEK"
     gene            complement(626..1900)
                     /locus_tag="PLY56_06945"
     CDS             complement(626..1900)
                     /locus_tag="PLY56_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CIA30 family protein"
                     /protein_id="HOJ21252.1"
                     /translation="MRYRNALFVFCVALLACASSAGAAQRGATFLIDSMDQIDQWTVS
                     RVKETPTVKLALCDDREGRPGAAFSVKADVGRLSALAMRRIQPDEAWNRYDGLSFWVK
                     GDGSENWANIRLQARNGQAAWVASFPLRQTEWHEVRLAWGDFVPVTARIDELGTAKGI
                     RPAEIDRVGFGKEWNFNIFHKSPEISFCIDDLAFVRGVKSSRRRVPIGKLPPLSSVVR
                     KLETGQPVTILTLGDSITWGTSAGGNDYAYPAVLASLLRERYPKAEITLVNRAIGGSS
                     TSRGREWLMRDVLGIEADLITVMMGYNERPAADEDVAAVTDRYLANLVRYVEEVAGVM
                     KKRPTCVLLAPTPGRQTNWMALDAYAQGVRELGKRHRNVMIADANAHMKQLGQEAYAK
                     LMADEAHPNPEGQKVMAEIVFRAITGQAPARH"
     gene            2336..3736
                     /gene="asnS"
                     /locus_tag="PLY56_06950"
     CDS             2336..3736
                     /gene="asnS"
                     /locus_tag="PLY56_06950"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOJ21253.1"
                     /translation="MILKGNTIGDILKKPAGTPVEAHGWVKTRRDSKGIHFIQLNDGS
                     SFTDLQVVVEEGAVPPEVLAGATTGASLRVSGELVESPAAGQAVELRARAIHVYGTAE
                     PTTYPLQKKGHTMEFLRQIAHLRARSNTFGAVFRVRNVLSNAIHRFFQERGFLYIHTP
                     IITTSDCEGAGAMFQVTTLDLMNLPRNEVGQVEYASDFFEKPAYLTVSGQLEAEALAL
                     AFTNVYTFGPTFRAELSTTPRHLAEFWMVEPEMAFADLDDDRRLAEEFLKYIITAVLD
                     EASEDLAFFNKRIDSTVLSTLEHVATSAFEHIPYSEAIRLLQKAQDQGRQWEFPVHWG
                     ADLQSEHERYLTEEVFKKPVVVTDYPKEIKAFYMRVNDDGRTVRAMDVLVPRIGEIIG
                     GSQREERYDVLRDRIREQGLNEEDYWWYLDLRRYGSAPHAGFGLGLERMMMYVTGMKN
                     IRDVIPFARTPGDAEF"
     gene            4138..4722
                     /locus_tag="PLY56_06955"
     CDS             4138..4722
                     /locus_tag="PLY56_06955"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ21254.1"
                     /translation="MLVVTIFLERLWAFSRMGRPPRELLHRVESRLTAGQWQEALRLL
                     DESPSPYARVAKAGLLRRQASPQEVSDMLTLACETEVASASRGLPVLGTIGNVAPFFG
                     LFGTVLGIMRAFQEVSHRGSAEAAAISGGIAEALVATALGLGVGIAAVIGNNWLQALV
                     EGFRLRLERTATEWVYFLQHLSEPVAERDREPVA"
     gene            4719..>5032
                     /locus_tag="PLY56_06960"
     CDS             4719..>5032
                     /locus_tag="PLY56_06960"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ21255.1"
                     /translation="MIRTRRGKESGTITGINITPFTDVCLVLLIIFLVTAHSFARESS
                     LSLNLPRAASARTPLPTSVTVKINRQGQLFLDEARVTLGTLGERLKEARARHGVELLV
                     IKA"
BASE COUNT          844 a         1719 c         1582 g          887 t
ORIGIN      
        1 acttctccac ctgcgccgga actctcggtg gcacataggg gctcacctgc aggtaatcga
       61 ggcgcacgcc tgccggcagc cagacccgga tctcctgctc ttgtccgttg aagtcgaact
      121 tgccggtagc ctgtttgcag ctttccggcc ggctccacgg cacgaagacc acccggcttg
      181 tcgggcggct gccatccact gagatcatca accggagcga ggcttgcttc ccggtcgctc
      241 ggcgcatcag ctcggtgcca tgggcgtctg tcgccgcgtg ggtgcggatc cagtagcgcc
      301 ccgcctgggg agctcgcacc cgaaaaacca ggtccggcgc ggtatccggc ttgcccacgt
      361 tgctggcgac gcccgccttg agcgcgactc cccgtttgct tgcgaagctc tcttgcgcca
      421 cgatctcggt ccggttcggg tcaagctgag cggcctctgc ctcgagcgtg attggcccgc
      481 cctgcgcggg cgcggcatcc gcgccggccc cgaaaagtgt tgtcaatatc attgtgctaa
      541 tccccaccac ccttgctccg aggtgcatgc cgccctccta ttcctccatc ctacctccgc
      601 gcccgactat ccctctggcg gccggtcagt gccgggcggg cgcctggcct gtgatcgcgc
      661 ggaaaacgat ctccgccatc accttctgcc cctccggatt ggggtgggct tcgtcggcca
      721 tcagcttcgc gtaggcctcc tgccccagct gcttcatatg ggcgttagcg tccgcgatca
      781 tcacgttccg gtggcgcttc cccagctccc tcactccctg agcataggca tccaacgcca
      841 tccagttggt ctgccggcca ggcgtcgggg caaggagcac gcacgtcggc cgcttcttca
      901 tcacgccggc cacctcctcc acgtagcgaa ccaggttggc caggtagcgg tcggtgacgg
      961 cggccacgtc ctcatcggca gccgggcgct cgttgtagcc catcatcacc gtgatcaggt
     1021 ccgcctcgat cccgagcacg tcgcgcataa gccactcgcg acccctggag gtggaggagc
     1081 cgccgatggc acgattgact agggtgatct ccgccttggg gtaacgctct cttagtaggc
     1141 tcgccagcac agcgggatag gcgtagtcgt tgccacccgc ggaggtgccc caggtgatcg
     1201 aatcgcccag cgtgaggatc gtgacaggct ggccggtctc cagcttgcgg accacgctgg
     1261 agagcggcgg cagcttgccg atgggaaccc ggcgccggct ggacttcacg ccccggacga
     1321 acgccaggtc atcgatgcag aacgagatct cagggctctt gtggaagatg ttgaagttcc
     1381 actccttgcc gaagccgacg cgatcaatct ccgcgggccg gatgcctttg gcggtgccca
     1441 gttcatcgat acgcgcggtc accggcacaa agtcgcccca tgccaggcgc acctcgtgcc
     1501 actccgtctg ccgcagcggg aaggaggcaa cccacgccgc ctggccgttg cgcgcctgaa
     1561 gccggatgtt cgcccagttc tccgagccat cgcccttcac ccagaacgac agcccgtcgt
     1621 accgattcca cgcctcgtcc ggctggatgc ggcgcattgc gagcgccgag agccggccca
     1681 cgtccgcctt caccgagaac gccgctcccg ggcggccttc acggtcgtcg cagagcgcca
     1741 gcttcaccgt tggggtctct ttcaccctgg agacggtcca ctggtcgatc tggtccatcg
     1801 aatcgatgag aaaagtggcg ccgcgctgtg ccgcgcccgc tgagctcgcg caggcgagca
     1861 gcgccacgca aaagacaaag agagcgttcc gatatctcac aagagcttct ccttcccggt
     1921 ctgggccttg ctagcttggc caccaagttc cgcgcgcggg ccgcaacctc ctgcctccgg
     1981 caccacctgg cgggcatgta ggtatccgca gatggacaca gatgagaaga gttgatgggt
     2041 tccggcatga tgccgggtgc aggctcgctt tcgactggca tgaccggttc gcgagtgcat
     2101 gatctccgca cctgtggagg ctccaggcgc ccacaccctc cgcatccgtg tctgtcccct
     2161 tcctccgtgg tatcggtccc ccgggtagcc ggccgccagg aggagcccag gcccgcacat
     2221 ctctccattg acagaggcgg cggttcaggg cataatgggg gcgactggat ttgaggcaag
     2281 ggcccgccgc gcaaggcagg ccgtggcccg gcgagcgttg ggggaaggaa cgacgatgat
     2341 tctgaagggc aacaccatcg gcgatatcct gaagaagccg gcgggcacgc cggtcgaggc
     2401 gcacggatgg gtgaagaccc ggcgcgacag caaggggatc cacttcatcc agctgaatga
     2461 tggctccagc ttcacggacc tgcaggtcgt cgttgaggag ggcgcggtcc ccccggaggt
     2521 gttggccggt gcgactacgg gcgcatcctt gcgcgtctcg ggcgagctgg tggagtcgcc
     2581 cgccgccgga caggccgtgg agctcagggc gcgcgccatc cacgtctatg gcacggccga
     2641 acccaccacc tatccgcttc agaaaaaggg gcacacgatg gagttcctgc gccagatcgc
     2701 gcacctgcgc gcgcgcagca acaccttcgg cgccgtcttc cgggtgcgca acgtcctctc
     2761 caacgccatc caccgcttct tccaggagcg gggcttcctc tatatccaca cgccgatcat
     2821 caccacctcg gattgcgagg gcgccggcgc catgttccag gtcaccacgc tcgacctgat
     2881 gaacctgccc cgaaacgaag tcggccaggt agagtacgcg agcgacttct tcgaaaaacc
     2941 ggcctacctt accgtgagcg gccagctcga ggcggaggcg ctggctctcg cgtttacgaa
     3001 cgtctacacc ttcgggccca ccttccgcgc cgagctttcc accaccccgc gccacctggc
     3061 ggagttctgg atggtcgagc cggagatggc gtttgccgat ctcgacgacg accgccggct
     3121 ggccgaagag ttcctcaagt acattatcac cgccgtgctg gacgaggcga gcgaggatct
     3181 cgcgttcttc aacaagcgga tcgacagcac cgtcctcagc acgctggagc acgtggccac
     3241 cagcgccttc gagcacatcc cctactccga ggcgatccgc ctcctgcaga aggcgcagga
     3301 tcagggccgc cagtgggagt ttcctgtcca ttggggcgcc gacctgcaga gcgagcacga
     3361 gcgctacctc accgaggaag ttttcaaaaa gccggtcgtg gtcaccgact accccaagga
     3421 gatcaaggcg ttctacatgc gcgtgaacga tgacggccgc actgtgcgcg cgatggacgt
     3481 gctcgtcccg cgcatcggcg agatcatcgg cggtagccag cgcgaggagc ggtatgatgt
     3541 cctccgcgac cgaatccggg aacaggggct gaatgaagag gactactggt ggtacctcga
     3601 cctgcgccgc tacggttcgg cgccccatgc cggcttcggc cttggcctcg aacgcatgat
     3661 gatgtacgtc accggtatga agaatatccg cgacgtgatc cccttcgcgc gcactccggg
     3721 ggatgccgaa ttctgagctg cgccggcgag ccattccttg ccaaggaggc gctcgaatgg
     3781 agcgcctcct ccagcgccag ggagcccccg cgcgcgcagg aaaccttctc tgcaaccgat
     3841 acgtcctcta agcattcgcc gtgtgaaccc cggaggccag gaacggcgcc ggcgagagcg
     3901 atccagatgc tcttcccaaa gcgcgcgttc gcggtgctcg ccgcgagtct gggcccggga
     3961 tcaggaagcc aggcaccagc cgcggctcat cgcgcgcatt gaacactccg ggtgtttcca
     4021 cggtctacct ggatcaggac actgctagaa cggggaaccc actgtatgcc ttcgcactca
     4081 ttcttgagcc tcatccagca gggaggaatc tccctcgtcc cgctggggat ctgctccgtg
     4141 cttgtggtca ctatcttcct ggagcgcctc tgggccttct ctcgcatggg ccggcccccg
     4201 cgagagctac ttcaccgcgt ggagagccgg ctcaccgccg ggcaatggca agaggcactc
     4261 cggctattgg atgagtcgcc cagtccctac gcgcgcgtgg caaaagcagg cctgctccgc
     4321 cggcaggcaa gcccgcagga ggtctcagat atgctcacgc tcgcctgtga gacggaggtg
     4381 gcgtccgcct cgcgcgggct gcccgtgctg ggcactattg ggaatgtcgc ccccttcttc
     4441 ggtctcttcg gcacagtgct cgggatcatg cgtgccttcc aggaggtctc gcaccgcggg
     4501 agcgcggagg cagccgcgat ctccggcgga attgccgagg cactcgtcgc cacggcgctt
     4561 ggtctgggcg tgggcatcgc cgcggtgatt ggcaacaact ggctgcaagc cctggtggag
     4621 ggcttccggc tgcgcctcga gcgaacggct acggagtggg tctacttctt gcagcacctg
     4681 agcgagcctg tcgccgagcg cgatcgggag ccggtcgcat gattcgaacg cgacgcggca
     4741 aggaatccgg cacgatcacc gggatcaaca tcacgccctt taccgatgtc tgcctggtgc
     4801 tgctgatcat cttcctggtg accgcgcaca gcttcgcgcg cgagtcgagc ctcagtctca
     4861 acctgccgcg ggcggcatcc gcgcgcacgc ccctgcctac ctcggtcacg gtgaagatca
     4921 accgccaggg ccagctcttc ctggatgagg cccgggtgac gttgggaaca ctcggcgagc
     4981 gactgaagga ggcgcgcgcg cgtcatggag tcgaactgtt ggtgatcaag gc
//
LOCUS       DAOSCV010000227         5025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10507_length_5025_cov_3.409859, whole genome shotgun sequence.
ACCESSION   DAOSCV010000227 DAOSCV010000000
VERSION     DAOSCV010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10507_length_5025_cov_3.409859"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLY56_06965"
     CDS             <1..800
                     /locus_tag="PLY56_06965"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ21256.1"
                     /translation="REYILDKAVSVLGRDELVDVGVFGDPSVAPWHAAIKLDGGAYVI
                     EDLETSPGGTLVNGQRVSRQVLREGDTIQLGATKILFHEKVGSAPARRARDVSATPSP
                     RVPAVADNICAFCGQEKDPRTGDCACTPVAPPAPGFAPAMEAAGATPGTGAVMPRLSE
                     MPATAAQARLVGISGPYAGQTIPLSGEVLTIGREAGRDLQLSGDGTVSRRHAVLRVED
                     GGYVIADEGSSNGTFVNGVRINAQLLRSGDRIRIGASEFVFEAGGAF"
     gene            797..1741
                     /locus_tag="PLY56_06970"
     CDS             797..1741
                     /locus_tag="PLY56_06970"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ21257.1"
                     /translation="MKDNLKIFSGSSNPELAEEICRCLDIPLGRVVRSRFANDNLFIQ
                     IEESVRECDVFVVQSSCPPVSEGIVELLILIDALRSASASRITAVLPYYPYVRSDKKD
                     QPRISITAKLMAQLLRAAGAGRVLTMTLHSPQIQGFFDIPVDHLQSTPVLCSYYAQLP
                     VIRECVAVAPDAGSAKRAGSFAQRLDIPLAFVDKRRLGNTDDVASRAVVGDVRDKHVI
                     IFDDEISTAGSLMGAVDVLKNAGAREIYVGCTHGVLCGPAIERIAASPVKEVVVTNTV
                     PLPPEKRHPKIKTLSVAPLFAEAIKRTHTGQSIHSLFL"
     gene            1746..2915
                     /locus_tag="PLY56_06975"
     CDS             1746..2915
                     /locus_tag="PLY56_06975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ21258.1"
                     /translation="MKVCLVYPNLPPVVDGVGEYTCHLAGEMAAQGAEVTVISGPVPE
                     GAGKVCPVSGNVRLGRTIHRWGVRGHGRLVGAIRETGCDVVVLQYTPYAYARCGLGVG
                     VALLPHILRVRLPCKVAVALHEPWIPFQGGPGRLLVAGWQRLATGYMVGACHRVIVTT
                     EKRRRQLQRLVFPVGGNVSVVPVSSNIPVVECDRAAARAHLGVGDGETLFAIFGMSHR
                     HRDYEAVYRCLHTLIAEGHACRLVNIGKIASPGRLQALCRLEKELGLEGYISWTGFCD
                     AEMVSRILQAADVAVMPERFGISTRQTTLMAALSHALPVVAWRGPETDPVLERSGAIV
                     FVNAGDEDGLTAAMRAAVLNPGTRNAMGMRGRELFEEHFSWPRVAELTLKVLGRP"
     gene            complement(2918..3886)
                     /locus_tag="PLY56_06980"
     CDS             complement(2918..3886)
                     /locus_tag="PLY56_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ21259.1"
                     /translation="MEVIAPAARPLGKSTREAFGEALRDLGYARPDVVVVDADLNTST
                     RTQLFAVAFPDRFYNVGIAESNLVGVASGLAACGKQVWIASFGVFLLANAFDQLRMSV
                     ALPRLNVKLVGTHTGITVGEDGPSQMAVEDMALACALAGVRVFVPADEVETRAMVQAA
                     AEIDGPVYIRCGRPPVPVVHESGCVFRPGRATVLREGSDLTLAANGIMVAASLDAAEE
                     LARQGLSARVLNLSSVKPIDEDALEQAARETGALVVAEEHLAHGGLGSIVARVLSARY
                     PVPLGFVNLGDTYAESGEPRALVERYGLTAGAIAQAARAVVLRKPT"
     gene            complement(3877..4722)
                     /locus_tag="PLY56_06985"
     CDS             complement(3877..4722)
                     /locus_tag="PLY56_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ21260.1"
                     /translation="MADAAALQAASLEALREKARVLRKHIITMTTAARSGHPSSSLSA
                     VEILVALYFGGILRHDPERPTWPERDRFILSKGHAAPALYAALAEAGYFPVEELATLR
                     RIGSRLEGHPILGKLPGVEASTGSLGQGLSIGVGHALAGKLDSLPYHVFVMTGDGEVD
                     EGQVWEAALSASKYRLDNLTCIVDHNGYQQTGSTRDVMPLSPLPDKWRAFGWHTLEVD
                     GHDLAAVLSTLAAARAFPGQPVAIIAYTRKGKGVSFVESDYAYHGKALSPEEADRALE
                     ELGWK"
BASE COUNT          823 a         1597 c         1739 g          866 t
ORIGIN      
        1 gtcgggagta catcctcgat aaggccgtca gcgtcctcgg gcgcgatgag ctggtggatg
       61 tgggcgtctt cggagatccc tcggtagcgc cctggcacgc ggcgatcaaa ctggacggcg
      121 gggcctatgt gatcgaggat ctggagacat cgccgggcgg caccctggtg aatgggcagc
      181 gcgtgtccag gcaagtgttg cgcgagggcg atacaatcca gctcggggcg acgaagatcc
      241 tcttccatga aaaggtgggt tcggcgcccg cgcgccgcgc gcgcgatgtg agcgcgacgc
      301 cgtctccgcg ggttccggcg gtcgccgaca acatttgcgc cttctgtggc caggaaaagg
      361 atccccgtac cggggactgt gcgtgtacgc cggtcgcgcc gccagccccg gggtttgccc
      421 cggcgatgga ggcggccggc gcaactccgg gaacgggcgc ggtgatgccc cggctctcgg
      481 agatgccggc gacggcggcg caggctcggc tggtgggtat cagcgggccc tacgccgggc
      541 agacgatccc gctatcgggt gaggtgctca ccatcggtcg cgaagcgggg cgcgatcttc
      601 agctgtcagg agatggcacc gtgtcgcgac ggcacgctgt gctgcgtgtt gaggacggtg
      661 gatacgtcat cgccgatgag ggcagctcga acggcacgtt cgtgaatggc gttcgtatca
      721 atgctcagct gctgcggagt ggcgaccgca tccgtatcgg cgcgagcgag ttcgtttttg
      781 aagccggagg tgcattctga aagacaatct caagatcttc tcgggcagtt cgaacccgga
      841 actagcggag gagatctgtc gctgtctcga tattccactg ggtcgcgtcg ttcgctcgcg
      901 ctttgccaac gataacctct tcatccagat cgaggagagc gtgcgcgaat gcgatgtgtt
      961 cgttgtgcaa tcctcctgcc cgcccgtgag cgaggggatt gtcgagctgt tgatcctgat
     1021 cgatgcgctc cgcagcgcgt ccgcgtcgcg gatcacggcg gtattgcctt actatcccta
     1081 cgtgcgctcc gataagaagg accagccacg gatctcgatc acggcaaagc tgatggcgca
     1141 gttgctccgc gcggcggggg ccgggcgcgt gctcaccatg acgctccact cgccgcagat
     1201 ccaggggttc ttcgatattc cggtcgacca cctgcaatcc acgccggtgc tctgcagcta
     1261 ttacgcgcag ttgccggtga tccgggagtg cgtcgcggtg gcgccggatg ccgggagtgc
     1321 caagcgcgcc ggcagctttg cccagcgcct ggatattcct cttgctttcg tggacaagcg
     1381 ccggctgggc aacaccgacg atgtggccag ccgcgcagtc gtgggcgatg tgcgtgacaa
     1441 gcacgtgatc atcttcgatg atgagatcag caccgctggc tcgctgatgg gcgcggtgga
     1501 tgtgctcaag aacgcgggcg cgcgcgagat ctacgtgggg tgcacgcacg gggttctgtg
     1561 tggcccggcc atcgagcgga ttgccgcgtc gccggtgaag gaggttgtcg taacgaacac
     1621 ggtgccgttg ccgccggaga agcgacaccc gaagatcaag acgctctcag tggcgccact
     1681 tttcgcggag gcaatcaagc gaacgcatac aggccagtcg atccactcgc tcttcctctg
     1741 agatcatgaa ggtctgtctg gtctacccca atctgccgcc agtggtggat ggggtagggg
     1801 agtatacctg ccacctggcg ggcgagatgg ctgcgcaagg cgccgaggtg accgtgatca
     1861 gcggccccgt tcccgaaggc gcggggaagg tctgcccggt ctccggaaat gtgcgtcttg
     1921 ggcgcaccat ccatcgctgg ggggtgcgcg gacacgggcg attggtgggc gcgattcggg
     1981 agacagggtg tgatgtggtc gtcctccaat acaccccgta tgcgtacgcc cgatgcgggt
     2041 tgggggtggg cgtcgcgctc ctgccgcaca tcctccgcgt ccggctgcct tgcaaggtgg
     2101 cagtggcgct gcacgagccc tggatcccat ttcagggcgg ccccgggcga ttgcttgtcg
     2161 cgggatggca gcgccttgcc accggctaca tggtcggcgc atgtcaccgt gtgatcgtga
     2221 ccacggagaa gcgccggcgg cagctccaac gcctcgtctt cccggtgggg ggcaacgtct
     2281 ccgtcgtgcc cgtcagctcg aatatccccg tggtggagtg cgaccgcgcg gctgcccgcg
     2341 cgcatctggg cgttggagac ggggagacgc tctttgccat cttcggcatg tcgcaccggc
     2401 accgcgacta tgaggcggta taccgctgct tgcacacctt gattgccgaa ggccacgcct
     2461 gccgcctggt gaacatcggt aagattgcca gccccgggcg gctgcaggcg ctgtgccgtc
     2521 tggagaagga actcggcctg gaaggctaca tctcgtggac cggtttctgc gatgccgaga
     2581 tggtctcgcg gatcctccag gcagccgacg tcgcggtgat gccggagcgc ttcgggatct
     2641 ccacgcgcca gacgacgttg atggcggctc tctcgcacgc cttgcctgtc gtggcgtggc
     2701 gtggcccgga gacggatccc gtgctggaac gcagcggcgc gattgtcttt gtcaacgcgg
     2761 gggatgagga tggcctgacc gcggcgatgc gcgccgccgt gctgaatccc ggcacgcgca
     2821 acgcgatggg catgcgcggg cgagagctgt tcgaggagca tttctcgtgg ccacgggtgg
     2881 cggagctgac cctcaaggtg ctggggcgcc cctgatctca ggtcggcttg cgtagcacga
     2941 ctgcgcgagc cgcctgggcg attgccccgg cggtgagccc gtatcgctcc accagggcgc
     3001 gcggctctcc ggactccgcg taggtatccc ccaggttgac gaaaccgagc ggaaccggat
     3061 agcgtgctga tagcacccgc gccacaatgc tcccaagccc tccgtgggcc aggtgctcct
     3121 ctgccacgac gagcgcgccg gtctcccgcg cggcctgctc tagagcatcc tcatcgatcg
     3181 gcttcacgct cgaaaggttg agaacgcgcg cggagaggcc ctgccgggca agttcctcgg
     3241 ccgcgtccag cgaggccgcg accatgattc cattggccgc gagcgtgagg tcgctcccct
     3301 ccctcagcac ggtggctctt ccggggcgaa aaacgcaccc tgactcgtgg accaccggca
     3361 ccgggggccg gccgcagcgg atgtaaaccg ggccatcgat ctcggccgcg gcctgcacca
     3421 tcgcgcgcgt ctcgacctca tccgccggga cgaagacgcg aacgccggcc agcgcgcagg
     3481 cgagggccat gtcttccacg gccatctggg atgggccgtc ttcgcccact gtgatccccg
     3541 tgtgcgtgcc caccagcttc acattcaggc gcgggagagc aacgctcatg cggagctggt
     3601 caaaggcgtt cgcgagcagg aagacgccga aggaggcgat ccagacctgc ttgccgcatg
     3661 ccgcgaggcc actggctacc ccgaccaggt tcgactcggc gatccccacg ttgtagaagc
     3721 gatccgggaa ggccaccgcg aagagctggg tgcgggtgga tgtgttgaga tccgcgtcta
     3781 ccaccaccac gtcggggcgg gcatagccaa gatcacggag ggcctcccca aaggcctctc
     3841 gggtcgactt ccccaacgga cgagcggccg gcgcaatcac ttccatccca gttcctccag
     3901 ggctcggtcg gcctcctcgg gcgagagtgc cttgccgtga taggcatagt cggactcgac
     3961 gaaggagact cccttcccct tgcgcgtgta ggcgatgatt gccacgggct ggccgggaaa
     4021 agcgcgagcg gccgcgagcg tggagaggac cgcggccagg tcatggccat ccacctcgag
     4081 ggtgtgccat ccgaaggcac gccacttatc cgggaggggc gataggggca tcacgtcgcg
     4141 ggtgctccct gtctgctggt agccgttgtg atcgacgatg caggtgaggt tgtccagccg
     4201 gtacttggat gcggagaggg cggcttccca gacttgcccc tcgtcaacct cgccgtcgcc
     4261 ggtcatcacg aaaacgtgat acggcaggct atcgagcttg cctgccaggg catgccccac
     4321 gccaatggag agcccctgac cgagagagcc ggtggatgcc tcgaccccgg gcagcttgcc
     4381 caggatcgga tgcccctcca ggcgcgagcc aatgcgccgg agcgtggcga gctcctcgac
     4441 cgggaagtag cctgcctcgg cgagggccgc gtagagcgcc ggagccgcgt gccccttaga
     4501 gaggatgaag cggtcgcgct ctggccaggt ggggcgttcg ggatcatgcc ggaggatccc
     4561 gccaaagtag agcgctacga ggatctctac ggcggagagt gagctgctcg ggtgccctga
     4621 acgcgccgcc gtggtcatcg tgatgatatg cttacgcagg acgcgcgcct tctcgcgcag
     4681 cgcctctaga gaagcggcct gcagcgccgc ggcatctgcc atcgggttcc ctcccggtga
     4741 tcggtccgcc gcgagaaaga agcaggccgg acgtggcgca ggaaaaccct ggcgcacatc
     4801 cggcccgcta cggacctacc acggggagtc taccccgtct cccacgttct acacccgaga
     4861 cagtggcttc ttaaccgcgg ccaagcagcc ggagcgcacg ggaagtgacg ttctcggggg
     4921 tgaacccaaa ctcgcgggcc agcacctcgg ccggggccga cgcgccgaag tgatcgagcg
     4981 cgatgatgtc gccgtcgatg cccacgtact tatgccaccc catgg
//
LOCUS       DAOSCV010000228         5007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10553_length_5007_cov_3.678110, whole genome shotgun sequence.
ACCESSION   DAOSCV010000228 DAOSCV010000000
VERSION     DAOSCV010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10553_length_5007_cov_3.678110"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..2923
                     /gene="mutS"
                     /locus_tag="PLY56_06990"
     CDS             311..2923
                     /gene="mutS"
                     /locus_tag="PLY56_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ21261.1"
                     /translation="MSDEKLTPMLRQYQSIKQQHPEVILLFRLGDFYEMFGDDAVTAA
                     PILDVVLTGRDAGALGRIPMCGVPHHSVDKYIAKLIAAGHRVAVCDQMEDPRFARGLV
                     RRQVTRVVTPGTVLEDSMLDAKANNYLAAVAGDVGGYGLAIVDISTGEFLVTQLTGET
                     AEADLMEELQRLAPAEVLVPADAEGLAGAIRKSLQLNVTPREKAAFAEATARERLMKH
                     FGVASLRGFGCEELPLAIEAADAALEYLRETHIASLGHLKSLGTYSAQQYMALDAATR
                     RNLELNLSLGEGTKTKSLLGVLDATLTPMGARLLRRWLEQPLLHVAEIRARQEVVAEL
                     HDHLLMREELRAALRKVGDLERLIARIATGTANARDLLSLRASLEQVPVAQEALASAE
                     AEPVRILRDGLRPLPEVTDLIARAIREDAPLTLREGGLIQEGFDEELDQLRRSSAEGK
                     AWIAGLEASERERTGIKSLKVGYNAVFGYYIEVSKPNLQFVPDNYIRKQTMANAERFI
                     TPELKEYEAAVLGAEEKVVQLEYRLFQQVREKVASRAESVLACARILAQLDVLAGLAQ
                     VASERYYVRPEVDDGDQIQITNGRHPVVEKYQAEERFVPNDVFLNTSDHRLLIITGPN
                     MSGKSTFLRQVALITLMAQIGSYVPADSARIGIVDRIFTRVGAQDDLASGQSTFMVEM
                     NETANILNNATERSLLILDEIGRGTSTYDGLAIAWAVAEYINAIGCKTLFATHYHHLN
                     ELEKSLEGVRNYRIAVKEEGDRIVWLRKIMPGGTDRSYGIEVARLAGLPAPVIARARE
                     VLTALEQNGASATGIPSDRAVIPTHAESLQLKLFDIAEHPVVEELRQLDITTMTPVEA
                     LTTLYNLQKRATRE"
     gene            3125..4987
                     /locus_tag="PLY56_06995"
     CDS             3125..4987
                     /locus_tag="PLY56_06995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ21262.1"
                     /translation="MRRVDPLLLAIVIAGLGLRLIGIGWGLPNIDRYHSYHPDEALQV
                     GATLRVDPLGGDFDPGFYNYGSLSFFLTRLAWEFSEGISPSSREPWALSARLHLVGRL
                     WTVFFGTLTLLLVFDIGRRLIRNDRRLSRFTPPADASGASSEPPLPEGGVLRRATYSV
                     LLTAAGRGPASETGVSYWRSELGARVAGLCAAGLLAMAPAHVAHSHYFTVDIGAAFWA
                     TAAVAAGLRLLEGPRIRDAILAGLCAGLAAATKYNTGLVLLAPLMALALSYRGRLAEQ
                     GPLPAEGAESACPPLGKRVPFGRGFLVPALACIGTCAVAFLAGCPGVALNTAAFLRDF
                     LFEAQHVRTGHGLVFVRTLPAWIYHITTSLRFGLGLPLLAIVLLAIAQAAKRRSPADL
                     VVAAWVIPYYLLIGSAQVKFLRYTLPLLPMLAAWAGVLCADWLLNTKRSRWRALATSA
                     LVGIGFWTLGAAVSYDLFFASPDPRDRAAGAIRSAVTPGATVALTTEPWFYTPPLVPW
                     NGGPQTRIEFEAARESWPYQFLVGGWSAERVARERPECFIASNLEEQIRDGVRLGLPE
                     AEQLVAILEREYRKEVFAPVASWPGTLGPGHAPEDWMYPAPTINVYLRDDIGGR"
BASE COUNT          825 a         1716 c         1611 g          855 t
ORIGIN      
        1 cagaccagac gattcgcgct ggcggatgag gcggcccgtg ggatgtccca gaatatggac
       61 gcaccccgtc cctaacgcgt tcaccacgcg cccaggtatc tcctttcgcc tcgtgccaaa
      121 gcgagggacg catcgggtta aatccagggc catacgccgc atggggcgag ggatggctcg
      181 cactctgcca ccgccgcttg cccgacaccg cccgagcgtg gtatcatctg aagagagttg
      241 tcccggttca gctggcgcac gtggcgcctg aaccccaacc cttgagatcc tgaacccagc
      301 gggagacctt atgtccgacg agaagctcac gccgatgctg cggcagtacc aatcgatcaa
      361 gcagcagcat ccggaagtca tcctcctgtt ccgcctcggc gacttctacg agatgttcgg
      421 cgacgacgcc gtcaccgccg cgcccatcct cgacgtcgtg ctcaccgggc gcgatgccgg
      481 cgccttgggc cgcatcccga tgtgtggcgt gccccaccac tccgtggaca agtatatcgc
      541 aaagttgatc gctgccgggc accgcgtcgc cgtgtgcgat cagatggaag accctcgctt
      601 cgctcgcggc ctggtgcgcc gtcaggtcac ccgcgtcgtc acccccggca ccgtcctcga
      661 agactcgatg ctcgacgcga aggcaaacaa ctaccttgcc gcggtcgctg gcgacgtcgg
      721 aggctacggg ctcgcgattg tcgatatctc cacgggcgag ttcctggtga cgcagcttac
      781 gggggagacc gccgaagcgg atctgatgga ggagttgcag cgcctcgcgc cggcggaggt
      841 gctggtgccc gccgatgcgg aggggctggc cggcgccatc cgcaaatcgc tgcagctgaa
      901 cgtcacgcca cgcgagaaag ccgcgttcgc cgaggccaca gcgcgcgaac gcctgatgaa
      961 gcacttcggc gtcgcctcgc tgcgcggttt cgggtgtgaa gagcttcccc tcgcaatcga
     1021 ggcagctgat gcagccctgg agtacctgcg cgagacgcat atcgcttctc tggggcacct
     1081 gaagtcgctc ggcacctact cggcacagca atacatggcg ctcgatgccg ccacccggcg
     1141 caacctggaa ctaaacctct ctctgggcga gggcaccaag acaaagagcc tgctgggcgt
     1201 gctcgacgcg actctcaccc cgatgggcgc ccgcctgttg cgccgatggc tagagcagcc
     1261 gctccttcac gtcgctgaga tccgggcgcg ccaggaggtg gtggcggagc tgcatgatca
     1321 cctgttgatg cgcgaggagc tgcgcgcggc cctgcgcaag gtgggcgatc tggagcggct
     1381 catcgcgcgt atcgccaccg gaaccgccaa cgcgcgcgat ctcctctcgc tgcgcgcctc
     1441 actggagcag gtgcccgtcg cgcaggaggc gctggcgtcc gccgaagcag aaccggtgcg
     1501 catcctccgg gatgggcttc gtccgcttcc cgaggtgacc gatctgatcg cccgtgccat
     1561 ccgcgaggat gcgccgctca cccttcgaga gggcgggctc attcaggagg gcttcgacga
     1621 ggagctcgac cagctccgcc gcagcagcgc cgagggcaag gcgtggattg ccggcctgga
     1681 ggcaagcgag cgcgagcgca ccggcatcaa gagcctgaag gtgggctaca acgccgtctt
     1741 tggctactat atcgaggtga gcaagccgaa cctccagttc gtgccggata actacatccg
     1801 caagcagacg atggcgaacg cggagcgctt catcacccca gagctgaagg agtatgaggc
     1861 ggcggtgctg ggagccgagg agaaggtggt ccagctagag taccgcctct tccagcaggt
     1921 gcgcgagaag gtggcatcgc gcgccgagag cgtgctcgcg tgcgcgcgga tcctcgctca
     1981 actcgatgtg ctcgccggct tggctcaggt cgcttctgaa cgctactacg tccgccccga
     2041 ggtggacgac ggcgatcaaa tccagatcac caacggccgg catcccgtgg tggagaagta
     2101 ccaggccgag gagcgtttcg tgcccaacga cgtcttcctc aacacgagcg accaccgcct
     2161 cctgatcatc accggcccca acatgtccgg gaaatccacc ttcctccgcc aggtggcgct
     2221 gatcacgctc atggcgcaaa tcggctcgta tgtgcccgcg gactccgccc gaatcggcat
     2281 cgtcgaccgg atcttcaccc gcgtcggcgc ccaggatgac ctcgcgtccg ggcagtccac
     2341 cttcatggtg gagatgaacg agaccgcgaa catcctcaac aacgccaccg aacgctcgct
     2401 tctcattctg gatgaaatcg gccggggcac aagcacctac gacggtctgg ccatcgcgtg
     2461 ggcggtcgcc gagtacatca acgccatcgg gtgcaagacg cttttcgcca cgcattacca
     2521 tcacctgaac gagctggaaa agagcctgga gggcgtgcgc aactaccgga ttgccgtcaa
     2581 ggaggagggc gaccgcattg tctggctgcg caagatcatg ccgggaggaa ccgaccgctc
     2641 ctacggcatc gaagtggccc gcctggcggg cctgcccgcg ccagtgatcg cgcgcgcaag
     2701 agaggttttg acagctctgg agcagaacgg cgccagcgcg actgggattc cgagcgaccg
     2761 cgccgtcatc cccacgcacg ccgaatcgct ccagctcaag ctgtttgata tcgccgagca
     2821 tcccgtcgtt gaggagctgc gccagctcga tatcacgacg atgaccccgg ttgaagcgct
     2881 gactacgctc tacaacctgc aaaagcgggc gacacgcgag taggctacgg gggagatcgg
     2941 atgccctggc ccaggggaga agcaaccggt cggcgcaagc acttcccggc gcccactcct
     3001 gaaagggcgc gaatgggccg cgaatcgttg aggcgccttc acgcggcgca tccggctggg
     3061 acccgtccac gagggatctg gccgatatgg tataatagtg ctgaggaaca atagggagag
     3121 ccggatgcgc cgcgttgatc ccctgctgct ggccatcgtg atcgccgggt tgggcctgcg
     3181 cctcatcgga attggatggg ggctgcccaa catcgatcgc taccattcct atcaccccga
     3241 tgaggccctg caggtcgggg ccacgctccg ggtagacccc ctgggcggag acttcgatcc
     3301 gggcttctac aactatggct cgctgagctt ctttctgacc cgcctggcct gggagttctc
     3361 ggaggggatc tcgccgtcaa gccgcgagcc ctgggcgctc tcggcgcggc tccacctggt
     3421 cggccggctc tggaccgtct tttttggcac cctcacgctg ctcctggtgt tcgatatcgg
     3481 ccggcggttg atccggaacg accggcgcct ctctcgcttc acgccgccag cggatgcgag
     3541 cggggcaagt agcgagccac ccttgcccga gggtggagtc ctccggcgcg ccacctacag
     3601 cgtgttgctc accgcggcag gccggggccc cgcgtcggag acaggggtct cctactggcg
     3661 atccgagttg ggggcgcgcg tggccggcct gtgcgcggcc gggctactgg cgatggctcc
     3721 cgcgcatgtg gcgcattcgc actacttcac tgtcgatatt ggcgccgctt tctgggccac
     3781 ggcagccgtc gccgcgggac tgcgcctgct ggaaggccct cggatccgcg atgccatcct
     3841 tgcaggactc tgcgccggcc tcgcggcagc caccaaatac aacaccggtc tcgtgctcct
     3901 cgcgccactc atggcgctgg cgctctccta tcgcggccgg ctggccgagc aaggaccact
     3961 ccccgctgag ggcgcagaga gcgcgtgccc gcccctgggc aagcgggtgc cctttgggcg
     4021 cggcttcctg gtgccggcgc tggcatgcat cgggacgtgc gccgtggcgt ttctggcggg
     4081 gtgccccggc gttgcgctca acacggctgc ctttctgcgc gatttcctct tcgaagcgca
     4141 gcatgtgcgg accggtcacg gcctggtctt cgtgaggact ctgcccgcct ggatctacca
     4201 catcaccacg tcactccggt tcgggcttgg cctgccgctc ctggcaatcg tgctcctggc
     4261 gattgctcag gccgcgaagc ggcgctcgcc ggccgatctg gtcgtggccg cgtgggtgat
     4321 tccctactac ctgctgatcg gctccgccca ggtgaagttc ctgcgctaca ctctcccgct
     4381 gctcccgatg ctcgcagcct gggcaggcgt cctctgcgcc gactggctcc tcaacaccaa
     4441 gcggtcgcgc tggcgcgccc tggccacctc tgccctcgtc ggcatcggct tctggacgct
     4501 aggcgccgcg gtctcctatg atctcttctt cgcttcgccg gacccgcgcg accgcgccgc
     4561 gggagcgatc cggtccgctg tcacgcccgg cgcaaccgtc gccctgacaa ccgagccgtg
     4621 gttttacacc ccgccgctcg ttccctggaa cggtggccct caaacacgga tagagttcga
     4681 ggcggcgcgc gaatcctggc cctaccaatt cctcgtcgga ggctggagcg cggagcgggt
     4741 ggcgcgcgag cgcccggagt gcttcatcgc cagcaacctg gaggagcaaa tccgcgatgg
     4801 ggtgcgcctc gggctcccgg aggcagagca gctggtcgcc atccttgagc gcgagtatcg
     4861 caaggaggtc ttcgccccgg tggcgtcttg gcccggaaca ctcggccccg gtcatgcccc
     4921 cgaggactgg atgtaccccg cgccaacgat caacgtctac ctgcgcgacg acatcggtgg
     4981 ccggtagtgc ctcatttcat accccac
//
LOCUS       DAOSCV010000229         4999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10574_length_4999_cov_4.664239, whole genome shotgun sequence.
ACCESSION   DAOSCV010000229 DAOSCV010000000
VERSION     DAOSCV010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10574_length_4999_cov_4.664239"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLY56_07000"
     CDS             <1..711
                     /locus_tag="PLY56_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21263.1"
                     /translation="FGNILMGVGGSLEVMLLMAEKEGFSPRIVERMQMMHRELMRGDN
                     IVKRLLSFARGSAPSTRPVSPSEVMEGVVTLCQAHPLSKGAKVTWCVDEGTPDILADP
                     SQLREVLMNLVLNALQATSKGGSVWLNARAEECDPDLERAQKPPQGASRLVVGSSTFR
                     GRGVSLTVADTGCGIPEEDMPRIFMPFFSRRLGGTQGTGLGLCVSLNMVDVMGGRICI
                     ESTVGQGTTFTIRLPAAA"
     gene            782..1735
                     /locus_tag="PLY56_07005"
     CDS             782..1735
                     /locus_tag="PLY56_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21264.1"
                     /translation="MSARRVLLVDDEEMVRLSLRDMLELAGYEVVGDAANGEEAVGLA
                     KAVRPDIILMDINMPRKDGVQAAQEIMASFPLPIVFLTAYSDSRNVSRASAAGAFGYL
                     VKPCRQADLAPAIETALARFREARAQQQDAEHVRWALETIRRLSREITTNPDIDGVVG
                     LALRCVLSAMKVPAVALFLARRDHLFIRAHLGVDDAGASAFCVPLGETLFWRAIVTGQ
                     PAAFTGSDEKSHRGTPVALPPPFGWAGSAVAAPLHDTEARGQADFRGCLAVFRPEEVA
                     FPAAQISILQALASELVVAFQMAKTRQALAWRLWQNAGPNA"
     gene            complement(1765..2076)
                     /locus_tag="PLY56_07010"
     CDS             complement(1765..2076)
                     /locus_tag="PLY56_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21265.1"
                     /translation="MAGTTTRFEDYCTHCRAGVLSRDSLVMVKGERLCLRCCQAHEEM
                     EARIRRLSGPLLLASVVIGGSLGYVVAKGVGVIPWIGLFPGVVVGLVAAGLIEGLLRR
                     Y"
     gene            complement(2368..2724)
                     /locus_tag="PLY56_07015"
     CDS             complement(2368..2724)
                     /locus_tag="PLY56_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ21266.1"
                     /translation="MAGTLQIGPVIRRLRERWRLTQQDVVEYARLERSSSYISSIETG
                     KTSPTLAELEALAVVFRTTLFEMIREAGGGSAEGAPADDLTPQLNALFRELSPEDQEL
                     ALEILRLLARRKPAGA"
     gene            3193..>4999
                     /locus_tag="PLY56_07020"
     CDS             3193..>4999
                     /locus_tag="PLY56_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ21267.1"
                     /translation="MIPCRKLLGAWAGWIALFCLTMSGAAADGQPMVMVGRCEAPRLD
                     GRLSDPCWRDAAVAAPFIRNDGSGPASQQTVVRLCWDERALYLGFQCFERALSPATQQ
                     LHLLRQDARRQDGPVFADESIELFLRPGGKGEYFHLATNMRGTRYDARGMDASFNASW
                     DVATTADAEAWYVEMAIPFQSLGVPAPRPGDRWTLNFCRTEQILKERSSWSGLQGAFH
                     VPEAFGEVLFSERAVPVQLVDLADLAASASALRLRIGKGDAPMQAELQTKDTTVRRSK
                     AAVPAGMGEVRVAYPVGREGGAGSARYEVTFADGTPVYRSPWFAQMGSVLTLTGHLVL
                     EGGDGTLLRNGQDAVRLPGGKRVPVSLRLEPGENVIALTAPGKTRLSGKLMCGAQEIP
                     VGEGWRWSEAPASGWDQPGFFAEEWRAVASAKGRLALPAAERVHLRRVIAVPVRRERF
                     WPLQQEVNLPRGSRMFLKPLFGCVGEPPADYVYYLDLPAPMRILATDNLDGVPLKPLQ
                     SRRIRREGRDYTRYRLEPVGTLAGGFTLELVWSNNAKTSHTYVPAFSLGGTFDWRDFA
                     IEVTSPQYAEQLRVLCLKWQNRGIHGTCWFDDISIC"
BASE COUNT          798 a         1552 c         1726 g          923 t
ORIGIN      
        1 ttcggcaaca tcctcatggg cgttggaggc agcctggaag taatgctcct catggccgag
       61 aaggaggggt tctctccgcg catcgtggag cggatgcaga tgatgcatcg cgagttgatg
      121 cgtggtgaca acatcgtcaa gcgcctcctc tcctttgcgc gcggctcagc gccttccacc
      181 cggcccgtat ccccgtcgga ggtgatggaa ggcgtggtga ccctgtgcca ggcacatccc
      241 ctcagcaagg gggccaaggt gacgtggtgc gttgatgagg gtacccccga catcctggcc
      301 gacccctcgc agctacgcga ggtgttgatg aacctcgtcc tcaatgcgct gcaggccacc
      361 tcgaagggtg ggagtgtctg gctgaacgct cgcgccgagg agtgcgaccc ggacctcgaa
      421 agagcgcaga agcctccgca aggtgccagc cggttggtgg tcggctcgag cacgttccgt
      481 gggcggggtg tctcgctgac ggttgcggat accggatgcg gcattccgga ggaggatatg
      541 cctcgcatct ttatgccctt cttcagtcgg cgcttggggg gcacccaggg caccggtctg
      601 ggactatgcg tcagtctcaa tatggtggat gtgatgggcg ggcgcatctg catcgagagc
      661 acggtgggcc agggcactac cttcacgatc cggcttcccg cagcggcgtg aagtggaaag
      721 actctgggcg gcggcctggg gccaggctgt cggcctgatg agtgagcggg ggtgacagag
      781 aatgagcgca cgccgggtgc tgctggtaga tgacgaggag atggtccggc tgagtctgcg
      841 cgacatgctg gagctcgccg gctacgaggt cgtcggggat gccgcgaatg gcgaggaagc
      901 ggttggcctg gcaaaggccg tacgcccgga tatcatcctc atggatatca atatgccgcg
      961 caaggatggt gttcaggcgg cacaggagat catggcgagc tttcccctgc cgatcgtctt
     1021 tctcaccgcc tattccgata gccgcaacgt ctcgcgggcc agcgcggcgg gcgcgtttgg
     1081 ctacctcgtg aagccatgcc gccaggcgga tctggcgccg gccatcgaga cggccctggc
     1141 gcgcttccgg gaagcgcggg cgcaacagca ggacgcggag cacgttcgct gggcgctcga
     1201 gacgatccgt cgcctctccc gtgagatcac caccaacccc gatatcgatg gcgttgtcgg
     1261 tctcgcgctg cgctgcgttc tgagcgcgat gaaagtgccg gctgtggcgc tcttcctggc
     1321 gcggcgcgat cacctcttca tccgtgctca ccttggagtg gacgacgccg gcgcgagcgc
     1381 gttttgcgtg cccctcggag agacgctttt ttggagggcc attgtcaccg gccagcccgc
     1441 cgccttcacc ggaagcgacg agaagagcca tcgcggaacg ccggtggcgc ttccaccgcc
     1501 gtttggctgg gccgggagcg cggttgccgc gcccctgcac gacactgaag cccgcggcca
     1561 ggccgatttc cggggctgcc ttgctgtctt tcggccagaa gaggtcgcct ttcccgcagc
     1621 gcagatcagt attctccagg cgcttgcgag cgaactggtc gtcgccttcc agatggccaa
     1681 gacacgccag gctctggcgt ggcgcctatg gcagaacgcg gggcctaacg cctgaacaaa
     1741 gcacctttcg ccagccgtga tgggtcagta gcggcgcagc aatccctcga tgaggccggc
     1801 ggccaccagg ccgaccacca cgccgggaaa caggccgatc catgggatca cgccgacacc
     1861 tttggccacc acatagccga gagagccgcc gatcaccacg ctggcgagga gaagcgggcc
     1921 ggatagccgc cggatgcggg cctccatctc ctcgtgggcc tggcaacatc ggaggcatag
     1981 gcgctcgccc ttgaccatga cgagggaatc acgcgagagt accccggcgc ggcagtgcgt
     2041 gcagtagtcc tcaaaacgtg tggttgtacc agccattggt gtttcccccc cgatagcttc
     2101 gcacgttctc tgcttcttgc aagcgttgtg ccacagctcg ctctttgggg ggctcgcacc
     2161 tcacctaggg gaacgccaca cgcgtctgtc agactttgat gggcgttacc catcctggcc
     2221 cgatgtgtgt aggcagcgag ttgccttcgt tattcacgag ggagagagga atctcctgca
     2281 agcgcgtggt gcccatcctg tcggcaggcg ttgggaaggg atgcgcgcgg cgaaccgaga
     2341 accgatcgaa aaggccgctt cgcgcagtca ggcgccggcc ggtttccgcc tggcaagcag
     2401 ccgcaggatc tccagggcca gctcctggtc ctcgggcgag agctcgcgga agagcgcgtt
     2461 gagttgcggc gtgaggtcat ccgcgggcgc gccttcggcc gatccgcctc ccgcttcgcg
     2521 aatcatctcg aagagcgtgg tgcggaagac cacggcgaga gcttcgagtt ccgctagcgt
     2581 cgggctcgtc ttgcccgtct caatgctcga gatgtagctt gaactgcgct ccaggcgcgc
     2641 atattcaacg acatcctgct gggtcagacg ccagcgctcg cgtagccgtc gaatgacggg
     2701 gccgatctga agggtgccag ccatcgtgag tgtttcgctc ctccttcgct attcgccacg
     2761 atgccgccac agtgcgtggg cacgcctcat ccggtggcgc aacccgactg cggctgcttg
     2821 attctaccgg aggcgcccgt gggtgtcaag gaaatccagt ttcgtcctgg gattccgaga
     2881 taaggaatcc gaagccacgg aggcagcacg caacccggcg cgcggcgata aatcaccgcg
     2941 ctgcttgcga gcgaagccct gccgggctgg gttggggata ctcccagatg tctcaaaacg
     3001 gcggtggctg ggaggagaag cctcgaaggg gctttgccag aaagtagcgc ggagcttcga
     3061 gctccgcgcg tgcgggtggc cttctcctta tgtcggaatc ccagagtttc gtcttactcc
     3121 ccccgcgcgc ggggggaggg gggccgggag ttctcgcgcc ctgaacccct cttctgtgaa
     3181 cggaggccag agatgattcc ctgccgaaag ctcctcggcg cgtgggccgg ctggatagca
     3241 cttttctgtt tgactatgtc gggcgcggcg gcggatggcc agcccatggt gatggtcggg
     3301 cggtgcgagg cccctcgctt agatggccgc ctgagcgatc cctgttggcg ggacgccgcg
     3361 gttgcggcgc ccttcatccg caatgatggg tcggggccgg cctcgcagca gacggtcgtg
     3421 cgtctctgtt gggacgagcg cgcgctgtac cttggcttcc aatgcttcga gcgcgcgctc
     3481 tcgcctgcca ctcagcagct tcatctcctg cggcaggatg ctcgcaggca ggatgggcct
     3541 gtcttcgccg acgagagcat cgagctcttc ctgcgcccgg gagggaaggg agagtatttt
     3601 catctcgcca ccaatatgcg cggcacgcgc tatgatgccc gggggatgga tgcctccttc
     3661 aacgcctcgt gggacgtggc caccacggcg gatgccgagg cgtggtacgt ggagatggcc
     3721 attccgttcc aatcgctggg ggttcccgca ccgcggcctg gcgatcgctg gacgctcaac
     3781 ttctgccgca cggagcagat tctcaaggag cggagcagct ggtcggggct acagggtgcc
     3841 ttccacgttc cagaggcgtt cggggaggtg ctcttcagcg agcgggctgt gcccgttcaa
     3901 ctggtggatc tggccgacct ggccgcctcg gcgtcggcgt tgcgtctccg tatcggcaag
     3961 ggcgacgcac ccatgcaggc ggagctgcag acgaaagaca ccacggttcg ccggtcgaag
     4021 gcagccgtgc cggccggcat gggcgaggtg cgtgttgcct atccggtggg ccgggaaggg
     4081 ggcgcgggtt cggcacgcta cgaggtgacc tttgccgatg gcactcctgt ctaccggtcg
     4141 ccgtggttcg cgcagatggg atcggtgctc acgctcaccg ggcatctcgt gttggaaggc
     4201 ggcgatggca cgctgctccg caacggccag gacgctgtgc ggcttcctgg gggaaagcgc
     4261 gtgccggtca gtctccgcct ggagcccggc gagaatgtga tcgcgcttac tgctccgggg
     4321 aaaacgcgcc tgagtggaaa gctgatgtgt ggtgctcagg agattccggt gggcgaaggg
     4381 tggcggtggt ccgaggcgcc tgcatcgggt tgggatcagc ctggattctt cgcggaggag
     4441 tggcgtgccg tagcgtcggc gaaggggcgg ctggcgctcc cggcggcgga gcgcgtccat
     4501 ctccggcgcg tgatcgcggt gcccgtgcgc cgagagcgct tctggccgct gcagcaggag
     4561 gtcaatctcc cccgtggcag ccgcatgttc ctcaagccgc ttttcgggtg tgtgggagag
     4621 ccgccggccg attatgtcta ctacctggac ctgcccgccc cgatgcgtat cctcgcgacc
     4681 gacaacctgg atggcgtgcc gctcaagcca ctccagtcca ggcgcatccg gcgcgagggg
     4741 cgagattaca cgcgctaccg cctggagccc gtgggcaccc tggccggcgg cttcacgctg
     4801 gagctggtgt ggtccaacaa cgccaagacg agccacacct acgtgcccgc cttctcgctc
     4861 gggggcactt tcgactggcg tgactttgcc attgaggtga cctcaccgca gtatgcggag
     4921 cagctgcgcg tcctctgcct caagtggcag aaccgcggga ttcacggcac gtgttggttc
     4981 gacgacatct cgatctgcg
//
LOCUS       DAOSCV010000230         4998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10582_length_4998_cov_3.288691, whole genome shotgun sequence.
ACCESSION   DAOSCV010000230 DAOSCV010000000
VERSION     DAOSCV010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_4998_cov_3.288691"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLY56_07025"
     CDS             complement(<1..138)
                     /locus_tag="PLY56_07025"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOJ21268.1"
                     /translation="MPPVKNCDVTECFYNQARKCHAGAINVGGSHPQCDTFTQSGTHA
                     SP"
     gene            361..1323
                     /locus_tag="PLY56_07030"
     CDS             361..1323
                     /locus_tag="PLY56_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21269.1"
                     /translation="MINLGLIGCGALGKIHAQCIAQIPEARFVAYVDKVEDAAEAALK
                     QFGGDYATTDVETLLCDDSVDAVYICTRHDSHASLAIAAARAGKHILIEKPLALRIEE
                     CEAVAEAVKRAGVTLMPAFKMRFYPLVQMAHEFIPNPQVMVGQMMDNRWGDNIWPQDP
                     VQGGANVHSQGCHTTDLLRFFAGSEPTRLWASGGTLTHPGHPCIDQCIASIQFANGHT
                     AAWIQGDAATSHFTSKFFFELFGNGKSVQLYDRFKKATFFDGQKTWVEERPDEEGFLL
                     ENREFISALQEGRQPVLTAHDGIQATRIVLAADRAIRTGEVQTL"
     gene            complement(1472..2896)
                     /locus_tag="PLY56_07035"
     CDS             complement(1472..2896)
                     /locus_tag="PLY56_07035"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ21270.1"
                     /translation="MIRFAGSLLGVVALVVGLAAGAGAQGVGDPPATPPPADVIISPG
                     DVITITVVGEPDLSRRVTVTDDGKVPVAYVGEVKVSGLKPHEAAAAIRKALGRYLRNP
                     EVTVDLVEANRASVFGKVMRPGTYVLPPQARALDLIQMAGGFDEGADRSSIRILRKGA
                     SLTADLAQYAATGDARFNPALQAGDVVSVGERPVLSISVFGQVLRPGVFTVPVDARVM
                     DAVQMAGGFTERADRARVVLLRSGAQIPVNLSNEVVTSGEGENPPLQAGDTITVPEKR
                     MLTVAVLGQVAKPSVYQVVEGTTVAEVIALAGGIVEGADLSLAKLIHRNQEASPIDLR
                     QALEDGNAAANIALEDGDTVSLPKPVIERSFGVLGAVRAGGYFVMERDRVTIMEALSR
                     AGGPTATADLRRAVLVRRSGDEITRTVLDVGKMLKGDERTANVTMGPGDVLFLDDKKE
                     PVFSRANILAAVGLLTSLIYAFGR"
     gene            3089..4834
                     /locus_tag="PLY56_07040"
     CDS             3089..4834
                     /locus_tag="PLY56_07040"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HOJ21271.1"
                     /translation="MALSMREVAEAFVALEKSSRRTTLVAILGDLLARAETAEMERLV
                     YLCQGRLGPPFSAPEIGLQAKLMMHAVADATGCTEEAIRHLYDERGDIGTVAGEILPA
                     EGRGISLMEVYHQLLAIATASGRGAQAQKIGLLADLLRRLSGPEARYVLRIAQGRLRL
                     GVGDATILDALATSAGDPSLKEAIERCYNLSSDLGLVARVLREEGEPGLRRFQARPGR
                     PVRPALAQRVASPEEALRRLGRCQAEPKYDGFRLQIHRVGDAVHLFSRNMEDVSGQFP
                     EIAEATLRQVGAESAILEGEAVGYLPETGEYLPFQVTVQRRRKHRVEAMQEAIPLRLF
                     AFDLLYADGREFLTAPLTERRAALERLVTPTPDSALALSPVLVTDDATELERFFDEMV
                     SEGLEGIMVKRLDGAYTAGARSFNWIKLKRGYRAELADTVDCVLVGYLYGRGQRARFG
                     IGSLLAAVYDEKRDRFQTVAKIGSGLSDEGWVKMRELLDQDSVPHRPARVDSVLQPDV
                     WCEPHYVVEVQADEITRSPLHAAGRDGEPTGYALRFPRMVGWIRADKHPEDATTVEEI
                     IEMFRIQRLRATSAL"
BASE COUNT          852 a         1668 c         1623 g          855 t
ORIGIN      
        1 aggcgaggcg tgtgttccgg actgtgtgaa cgtgtcgcat tggggatgag agccgcccac
       61 gttgattgcg cccgcatggc atttgcgcgc ctgattatag aaacactcgg tcacgtcaca
      121 gttcttcaca ggtggcatca tcggctgggt cccctttcgg aaagcgagca tcccggcgcg
      181 cccggggctc cggctctctg ccctgtgccc gcgcgacgca gggtggccag agataccgcc
      241 acaagagcac gcgggtccca cgatgtatac ccactccaca tcgcgtagaa acggtccgga
      301 ggccggcagg cgtttccctg gggcgtagcg aatggaatcg ctgcaggagg aagtaagtcc
      361 atgatcaacc tgggtcttat cggctgcggc gcgcttggca agattcacgc ccagtgcatc
      421 gcacagatac ctgaagcgcg tttcgtcgcc tatgtagata aggtggagga cgcggcagag
      481 gctgcgctca agcagttcgg tggcgactac gcaacgacgg acgttgagac gctgttgtgc
      541 gacgacagcg tggatgccgt gtacatctgc acgcggcacg actcgcatgc ctcgcttgcc
      601 atcgcggccg cgcgagcagg caagcatatt ctgatcgaga agcccctcgc gctccggata
      661 gaggagtgcg aggcggtggc cgaagccgtg aagcgcgccg gcgtcaccct gatgccggcg
      721 ttcaagatgc gcttctaccc gctggtgcaa atggcccacg agttcatccc caacccgcag
      781 gtgatggtcg ggcagatgat ggacaaccga tggggggata acatctggcc gcaggatccg
      841 gtgcaagggg gcgccaacgt ccatagccag ggatgccata ccaccgatct cctccgcttc
      901 ttcgccggaa gcgagcccac tcgcctctgg gcgtccggcg gaacgttgac tcatccgggg
      961 cacccctgta tcgaccagtg catcgcctcc attcagtttg ccaatggcca caccgccgcc
     1021 tggattcagg gcgatgccgc cacgagccac ttcaccagca agttcttctt cgagctgttc
     1081 ggcaatggca agagcgtgca gctgtatgac cgctttaaga aggccacctt cttcgacggc
     1141 cagaagacgt gggtcgaaga gcgcccggat gaggagggct tcctgctaga gaaccgcgag
     1201 tttatcagcg cgctccagga aggccgccag ccggtgctta ccgctcatga tggcatccag
     1261 gcaacgcgca tcgtcctcgc tgccgatcgc gccatccgca caggcgaggt gcagacgctg
     1321 tagctgctgc cagcggcgtg tctgagcgcc cggctatcga gcgttccgac gggtgaaacg
     1381 ccgcggtcgt taccactgcg ggggaaggct tcctgtgaag ttccaggcag ccttcccccg
     1441 cgcgcgtgtt catccctgcg agtgaccgcg cctatcttcc gaatgcatag atcagcgatg
     1501 tgagcaggcc gacagccgcg aggatattgg cccggctaaa cacaggctcc ttcttgtcgt
     1561 cgaggaagag gacatcgccc gggcccatcg tcacattggc agtccgctca tcccctttga
     1621 gcatcttgcc cacatcgagc acggtgcggg tgatctcgtc acctgaccgc cggacgagga
     1681 ccgcccggcg cagatccgcc gtggcggttg gcccgccggc acgcgagagg gcttccatga
     1741 tcgtcacgcg gtcgcgctcc atgacgaagt agccgcccgc gcgcacggca ccgagcacgc
     1801 cgaaactccg ctcgatgaca ggcttgggga gcgagacggt atcgccatcc tccagcgcga
     1861 tgttggcggc ggcgtttcca tcctccaacg cctggcgcag gtcgatgggg ctcgcctcct
     1921 gatttcggtg gatcagcttc gccagcgaga ggtctgcgcc ttcaacgatc ccccccgcga
     1981 gggcaatcac ttcggcgacg gttgttccct ccaccacctg gtagaccgag ggctttgcca
     2041 cctgccccag caccgcgacg gtcagcatcc gcttttcggg cacggtgatc gtatcgcctg
     2101 cctgcagggg tgggttctcc ccctccccgc tggtgaccac ttcgttcgag aggttcaccg
     2161 ggatctgcgc gccgctgcgg agcaagacca cacgcgcccg gtcagcgcgc tccgtgaagc
     2221 cgcccgccat ctgcacggca tccatcacgc gggcatccac gggcacagtg aagacccccg
     2281 gacgcaacac ctggccaaac accgagatag agagcacggg gcgctcgcct acgctcacca
     2341 catcgcctgc ctgcagcgcc gggttgaagc gtgcatcgcc agtggccgcg tactgagcca
     2401 gatccgcggt gaggctggcc cccttgcgca aaatccgaat gctcgagcgg tccgcgccct
     2461 catcgaagcc gcccgccatc tggatcagat ccagcgcccg cgcctgtggc ggcagcacat
     2521 aggtgcccgg cctcatcacc ttgccaaaga ccgaggcacg gtttgcttcg accaggtcaa
     2581 ccgtcacctc ggggttgcgc aggtagcggc ccaacgcttt gcgaatcgcc gcggcggcct
     2641 cgtgcggctt caggccagag accttgacct caccaacgta agcaaccggc accttcccat
     2701 catcggtgac ggtgacacga cgcgacaggt cgggctctcc cacgaccgtg atcgtgatca
     2761 cgtcgccggg agagatgatc acgtcggcgg gtgggggggt ggccggtggg tctccaacac
     2821 cctgcgcgcc agcgccggca gcgaggccaa ccaccagcgc caccacgccc agtagtgatc
     2881 ctgcaaatcg tatcatactc tctcctccct ctccgggaaa gccgatgcaa gccacctgcg
     2941 cgggtgcaaa aggcatgccg gagcccctgc gacttcgggt ccggcatgcc cccagccctc
     3001 cgcatctgga agccgggagg atggtttcgt gcctctcttc ggaattggca gtgcccctcg
     3061 ctgcgccctg gggtatgagg gagatacgat ggcactctcg atgcgggaag tcgcggaggc
     3121 gtttgtggcg ctggagaaga gcagccgccg gaccacgctg gtggccatcc tgggggatct
     3181 cttggctcgg gcggaaaccg cggagatgga acgcctcgtc tacctctgcc aggggcgctt
     3241 agggcccccc ttcagtgctc ctgagatcgg ccttcaggcg aagctgatga tgcacgccgt
     3301 cgccgacgcc accggatgca ccgaggaggc gatccggcat ctctacgatg agcggggcga
     3361 catcggaacg gtggccggtg agatcctgcc tgcggaggga agaggtatct ctctgatgga
     3421 ggtgtaccac cagctcttgg ccatcgccac ggcatccggc cgcggggcgc aggcgcagaa
     3481 gatcgggctg ctggcggatc tcctgcgccg cctctccggg cccgaggcgc gctacgtcct
     3541 ccggatcgcg cagggccggc tccgcctggg cgtgggcgat gccaccatat tggacgctct
     3601 ggccacttca gcaggcgatc cctcgctcaa ggaggcgata gagcgttgct acaacctctc
     3661 ttccgatctg ggtctggtgg cgcgcgtctt gcgcgaggag ggcgagcccg gcctccgaag
     3721 attccaggcc cgccccggcc ggcccgtgcg tccggcgctg gcccagcgcg tggcttcccc
     3781 cgaggaggca ctgcgccgcc tcgggcggtg ccaggccgag ccgaaatacg atggcttccg
     3841 cctccagata caccgggttg gcgatgcggt ccatctcttc tcacggaaca tggaggacgt
     3901 ttcggggcag tttccggaga tcgccgaggc cacgctgcgc caggtgggag ccgagtctgc
     3961 catcctcgaa ggtgaagccg taggctacct gccagagacg ggcgagtacc tgccgttcca
     4021 ggtgaccgtc cagcgccggc gcaaacaccg cgtcgaagcg atgcaggagg cgattcccct
     4081 ccgcctcttc gcttttgacc tcctctatgc cgatgggcgc gagttcctca ccgccccgct
     4141 caccgagcgt cgtgccgccc tggagaggct cgtcacgccc acccccgact ccgccctggc
     4201 cctctcgccg gtcctggtca cggacgacgc caccgaactg gagcgcttct tcgacgagat
     4261 ggtctccgag ggcctggagg ggatcatggt gaagcggttg gatggcgctt acacggctgg
     4321 cgcgcgctcc ttcaactgga tcaagctgaa gcgcggttac cgggcagagc tggcggatac
     4381 cgtcgattgc gtgttggtgg gctacctcta cgggcggggg cagcgcgcgc gctttgggat
     4441 cggctcgctc ctcgccgctg tctatgatga gaagcgcgac cgcttccaga cggtcgccaa
     4501 gatcggctcg ggcctctcag atgaggggtg ggtgaagatg cgcgagcttc tggatcagga
     4561 ctccgtgccc caccggccgg cgcgcgtgga ttcggtgttg cagccggatg tatggtgcga
     4621 gccgcactat gtcgtggagg tgcaggcaga cgaaatcacg cgcagcccgc tgcacgcggc
     4681 cggccgcgat ggcgagccca ccggctacgc cttgcgcttc ccgcgcatgg tcggctggat
     4741 tcgggccgac aaacaccccg aggacgcgac caccgtggag gagatcatcg agatgttccg
     4801 catccagcgg ctgcgagcaa cctcggcctt gtaggctcaa tcccacccgg cgacccgcca
     4861 gcgcacgccg gcgcgcggga tagttacgtg cgacagcgcc tcggcatcct cgggagcgtg
     4921 gccatagaga cggaggaagt ccgcctccag ttcctcttcc agggcggtcg gtgcggtcag
     4981 gggtagctcc acccaggt
//
LOCUS       DAOSCV010000231         4959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10700_length_4959_cov_3.356036, whole genome shotgun sequence.
ACCESSION   DAOSCV010000231 DAOSCV010000000
VERSION     DAOSCV010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10700_length_4959_cov_3.356036"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLY56_07045"
     CDS             complement(<1..194)
                     /locus_tag="PLY56_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21272.1"
                     /translation="MKPSRRQVVWALLLLLLVAAATIGIVASGRRGDRGVGRSLGGVL
                     CGLLGGAGTFFGLQGLLHRFV"
     gene            complement(181..1113)
                     /locus_tag="PLY56_07050"
     CDS             complement(181..1113)
                     /locus_tag="PLY56_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOJ21273.1"
                     /translation="MHASFRGACLLLAVALSFAGCRQQEPPVAPARVPAQAAVIDDLG
                     RPWDGHLPARRIVSLAPNVTEIVCAVGGQEYLVGIDTASDYPPAVKRIPQVGEFLRPS
                     IERIVSLRPDAVIVSSATLPAPEADRFAQTLRAPVFVLNPVHLEDVCRNVRIIGQLLG
                     LEQEAEAVAAKTERRLADVARRAGGQPRRRVFLEIWHEPLTTVGGGTFLDDLIRLAGG
                     QNVAASQKKPFPTFSLEALLAANPEVYIVTTGMGAAPDPAKRPGFGALRAVRNRRVVS
                     VPGDPVMRPTPRLAEGAEHLLRAIHPEAQADEAE"
     regulatory      complement(1195..1344)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1727..3145
                     /locus_tag="PLY56_07055"
     CDS             1727..3145
                     /locus_tag="PLY56_07055"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HOJ21274.1"
                     /translation="MEKIPTGIEGLDLILMGGIVRGHAILVEGTPGAGKTTVGIQFIH
                     EGVVRYGEAGLIISFEQFPAQLYRDAANFGWDLRALEAQNKVRVCCTSPEVLRDQLQK
                     PDGLIDEWIQEMGARRILIDSISHLDTLSADPMERRVLAVSLLNALKQRGLTVLVTKE
                     VTSEEGYGCSFEEYVVDAVIRVSNTADRETGRRQRHVEVLKSRGQDHISGRHYFRFGP
                     EGIEVFPLQVVPTRQAVPGEAALLPTGVAGLDHLLDGGLRQASSLLLSGESGTGKTIL
                     GLQFLQEGASLGEKGLLFLYQETPEQVQQVAASFGWDLAHLEAEGLLTVVYAPFAELS
                     LAEHLWVVRVQLQETGARRVVFDSLSAMLHEVSQQPHMAKERTDQLVRLTKGLGAAAI
                     FIAEVPAGSSRISTYGVEESLVDSVVLLRSTRDGLRRKRGIEVFKARGANHVMGEHRM
                     RITPSGIKVFYRPARGAERDDA"
     gene            3135..3896
                     /locus_tag="PLY56_07060"
     CDS             3135..3896
                     /locus_tag="PLY56_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HOJ21275.1"
                     /translation="MMPSADGGRREAPQSASPARYVHFAPVDAILAEPLPFGTAWQVE
                     GDSGIGKTILASHFAIQGLRRGESVLFVACDDAPQRVRASLQRFGFSVGSYERQGSFV
                     LVDAFSEGGDEPYLLYDRSDPEELAYLLAEGLSQVARPTRVIIDSMTSVAAYLAPRDL
                     IHLVYEKNRILKEPDVVLLDLYLADAMDPRETNSVSNAYDVSLRLFYAEDRGGIPRRN
                     LQIRKVRGGKFDPRPFPFSIHREHGVLVDSDYYHR"
     gene            4002..4373
                     /locus_tag="PLY56_07065"
     CDS             4002..4373
                     /locus_tag="PLY56_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ21276.1"
                     /translation="MKELRITTEPTKAGGHAVQVVRVAGYIDAHSFPRFEQEMLALIN
                     QGSYRLLLDLREATYICSTALGLLMSVFRQVRHNAGDLVIAGMSDKIANIFNLLGFSK
                     LIRAFSTEEEALQCLAMTEGQ"
     gene            4370..4852
                     /locus_tag="PLY56_07070"
     CDS             4370..4852
                     /locus_tag="PLY56_07070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21277.1"
                     /translation="MSTQRERRDLALVVPGETDYLYLIRDFVCAVARKMGFPDQRVGE
                     IEIAVDEACANIIEHAYSKVPSAGRVIVARYPDGAMQAITLRVSVFSNRIELRILDQG
                     PDFSISELGDLDLSAFTTGDRKRGMGTYIIRNFMDEVHHTYHPGVGNELTLVKYLSQA
                     "
BASE COUNT          877 a         1526 c         1693 g          863 t
ORIGIN      
        1 acgaagcggt gcagaaggcc ttgcaggccg aagaacgtgc ccgcgccgcc aagaaggccg
       61 cacaggacgc cgccaaggct gcgcccgaca ccacggtcgc cccgccgccc gctggcaacg
      121 atgccgatgg tggcggcggc taccagcagc aggagcagaa gcgcccagac gacctgcctt
      181 ctactcggct tcatcggctt gcgcctccgg atggatggcc cgcagcaggt gctcggcgcc
      241 ctcggccagg cgtggcgtcg gccgcatcac gggatcgccg ggaacagaga cgacgcgccg
      301 gttgcgcacg gcgcgcaggg cgccgaaacc aggccgcttt gccgggtccg gcgcggctcc
      361 cattccggtg gtcacgatgt acacctccgg attggcggcg agaagcgcct ccaggctgaa
      421 ggtggggaag ggcttcttct gcgacgcggc gacgttctgg cctcccgcca ggcggatcag
      481 atcatccagg aaggtgccgc cccctacggt ggtaaggggt tcgtgccaga tctcgaggaa
      541 gacacgccgc cgcggttgcc cgccggcccg gcgtgcgacg tccgccaggc ggcgctccgt
      601 cttcgcggcg acggcctccg cctcttgctc cagtccgagc agctggccaa tgatgcgcac
      661 gttgcggcag acgtcctcca agtgtaccgg gttgagcacg aagaccggag cgcgaagcgt
      721 ctgggcgaag cggtcggcct caggagcggg cagggtggcg ctagagacga tcacagcatc
      781 cgggcgcagc gagacaatcc gctcgatgct agggcggagg aactctccaa cctggggaat
      841 gcgcttcacg gcgggaggat agtcggatgc ggtatcgatc ccgacgaggt actcctggcc
      901 tccgaccgcg cacacgatct ctgtcacgtt cggggcgagc gagacgatgc ggcgtgccgg
      961 cagatggcca tcccaaggcc tcccgagatc atcgatcacc gccgcttgtg cgggcacgcg
     1021 cgctggagcg actggcggtt cttgctggcg gcacccggca aaggagagag cgacggccag
     1081 caggaggcag gctccccgaa atgacgcatg catccggcat tcctcatctt ggggagtggt
     1141 gggctggacc gccggcggca gaggcagcgt cactcccccc tttcacgcga aggaagagaa
     1201 tgaccggcag cgggctgggt gtctgactcc cgcagggcgt ctgcggttac agtagcggga
     1261 ctgcgccgga gttgcaccgg acttccccat ttcagcccgt ggcggcttgc gcccccatga
     1321 gccacccgcg ccgttgcccc ggcgcctgcg cgcgtggcag ccgtccgggg tgtcgcgctg
     1381 attatagcgc gcccgccccc gcagtgtcaa ggcgcggttg gccccgcgcc ggcgtcgata
     1441 cagtcgatac cacggctgaa gggagcggac gcggatggga aagcggggga tcccccggcc
     1501 tcctcagcca cgaacgtgat gaaccttcaa gaataagccg gccatgccga aaagaacatt
     1561 gcctgtccct aacactacat taaagaccag cggatttctt ctggctccaa tgcgtccgtg
     1621 ggatacacgc ggcgtgggcc ctgggcggtt gcgtcgggga gcgcaggctt gaccaattat
     1681 gcgccaggaa gtataatctg cctaacaggc ggggggatga ttcggaatgg aaaagatccc
     1741 tacagggatc gaaggcctcg acctcatctt gatgggcgga atcgtgcggg gtcatgcgat
     1801 ccttgttgag gggaccccgg gcgcggggaa gaccacggtt gggatccagt tcatccacga
     1861 gggagtcgtc cggtatggcg aggcgggact gatcatcagc ttcgagcagt tcccggctca
     1921 gctctaccgc gacgcggcca acttcgggtg ggaccttcgc gctctggaag cccaaaacaa
     1981 ggtgcgcgtc tgctgcacct ccccggaagt gttgcgcgac cagctccaga agccagacgg
     2041 cctgatcgat gagtggatcc aggagatggg agcccgccgc atcctcatcg atagcatcag
     2101 ccatctcgat acgctcagcg ccgaccccat ggagcgccgg gtgctcgccg tcagcctcct
     2161 caatgcgctg aagcagcgcg gcctgacggt tctggtaacc aaggaagtca cctcggagga
     2221 ggggtatggc tgctccttcg aggagtacgt ggtcgacgcc gtcatccggg ttagcaacac
     2281 cgccgaccgg gagaccggcc gccgccagcg gcatgtcgag gtactcaagt cgcggggcca
     2341 ggaccatatt tcgggccggc actacttccg cttcgggccg gaagggatcg aggtcttccc
     2401 cctgcaggtg gtacccacgc gccaggccgt gcccggagaa gcggcgctct tgccgacagg
     2461 ggtcgcgggc ctcgatcacc tgctggatgg cgggttgcgg caagcttcca gcctgcttct
     2521 gagcggcgag agcggcacag gaaagacgat cctcggcctc cagtttctcc aggagggcgc
     2581 ttctttgggc gagaaggggc tcctcttcct ctaccaggag acgcccgagc aggtgcagca
     2641 ggtggcggca tccttcggtt gggacctggc tcatctggaa gcggagggtc tgctgaccgt
     2701 ggtctacgcg cctttcgccg agctgagcct tgccgagcac ctctgggtgg tgcgggtgca
     2761 gctgcaggag acgggcgccc ggcgggtggt gttcgactcg ctatcggcga tgcttcacga
     2821 ggtgagccag cagccgcaca tggccaagga gcgcaccgac cagttggtgc gcttgaccaa
     2881 ggggctaggg gctgccgcca ttttcatcgc cgaggtgccg gccggcagct cgcgcatcag
     2941 cacctacggc gtggaagagt cgttggtcga ttcggtggtc ctattgcgca gcacgcgcga
     3001 tggcctgcgg cgcaagcgtg gcattgaggt cttcaaggcg cggggtgcca accacgtcat
     3061 gggcgagcac cgcatgcgga tcacgccgtc aggtatcaag gtcttctacc ggccggcacg
     3121 aggagcggaa cgagatgatg cctagcgcgg atggcgggag gagagaagcg ccccagagcg
     3181 caagcccggc gcgctacgtg cacttcgcgc cggtggatgc gatcctggcg gagccgctgc
     3241 cgttcggcac ggcatggcag gtggagggcg attcggggat cggcaagacg atcctcgcct
     3301 cgcacttcgc tattcagggg cttcgccggg gcgagtccgt gctcttcgtg gcgtgtgatg
     3361 atgccccgca gcgggtgcgt gccagccttc agcgcttcgg cttctccgta ggtagctacg
     3421 agcgccaggg gagcttcgtc ctggtggacg ccttttccga ggggggcgac gagccgtatc
     3481 ttctctatga tcgctcggat ccggaggagc tcgcctacct tctagccgag ggcctctctc
     3541 aggtggcccg gcccacccgg gtgattatcg actcgatgac ctctgtggcg gcctacctgg
     3601 cgccgcgcga cctgattcac ctggtttacg agaagaaccg tattctcaag gagccggatg
     3661 tggttctcct ggatctttac ctcgcggatg cgatggatcc acgcgagacg aactccgtgt
     3721 ccaacgcgta tgatgtatcg ttgcggctgt tctacgccga ggaccgcggg ggaatccccc
     3781 gacgcaacct ccagatacgc aaggtacgcg gaggcaagtt cgacccgcgc ccgtttccat
     3841 tcagcattca ccgggagcac ggggtgttgg tcgatagcga ttactaccat cgctgaggct
     3901 gatggatcta acagttggaa tacacgcttg cccggcgtga tgagaggcgc cgggattccg
     3961 taggccggtg cagatgaccg atccggcagg agtggctgtt catgaaagag ctgaggatta
     4021 ccaccgaacc gacgaaggca ggtgggcacg cggtacaggt tgtacgcgtg gccggctata
     4081 ttgacgcgca ctctttcccg cgcttcgagc aggagatgct cgccctgatc aaccagggga
     4141 gctatcgatt gctgctcgac cttcgcgagg caacgtacat ctgcagcact gcgttgggcc
     4201 tgttgatgag cgtattccgc caggtcaggc acaacgccgg cgacctggtg atcgcgggga
     4261 tgtccgataa gatcgccaat atcttcaatc tgcttggctt ctccaagctg attcgcgcct
     4321 tcagtacgga ggaggaggcg cttcagtgcc tggctatgac cgagggacaa tgagcacaca
     4381 acgcgagcgc cgggacctgg ctctggttgt gcccggcgag acggattacc tatacctcat
     4441 ccgtgacttt gtctgtgccg tggcacgcaa gatgggcttc ccggaccaac gcgtgggcga
     4501 gatcgagatc gcggtggacg aggcgtgcgc caacatcatc gagcacgctt acagcaaagt
     4561 gccttccgct ggtcgcgtga ttgtcgctcg ctaccctgat ggggcgatgc aggcgatcac
     4621 gctgcgcgtc tccgtcttct ccaaccggat tgagctgcga atcctggatc agggtccgga
     4681 cttctccatc agcgagctgg gcgatctgga tttgagcgcg ttcaccacgg gcgaccggaa
     4741 gcgagggatg ggcacctaca tcatccgcaa cttcatggac gaggtgcatc atacctacca
     4801 tccgggagtt gggaacgagc tgaccctcgt caagtacctt tctcaagcgt aggaggggtg
     4861 catgcgccgg ggcgaaggag gggctatcgt ggagccagag gagcatccgc tgtgtgcccg
     4921 ctaccgggat ctgctggccg gctacctgga tggcgggga
//
LOCUS       DAOSCV010000232         4956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10717_length_4956_cov_3.170986, whole genome shotgun sequence.
ACCESSION   DAOSCV010000232 DAOSCV010000000
VERSION     DAOSCV010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10717_length_4956_cov_3.170986"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..100)
                     /locus_tag="PLY56_07075"
     CDS             complement(<1..100)
                     /locus_tag="PLY56_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ21278.1"
                     /translation="MPTISQLVRKGRRKVQPTSKSPALKGDPQRRGV"
     gene            951..1607
                     /locus_tag="PLY56_07080"
     CDS             951..1607
                     /locus_tag="PLY56_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21279.1"
                     /translation="MRRWTRDDIAMEILQLYASGGELNYSSMAETQPSLLRAATRHFG
                     SWRSAVEYAGLSYDQIRKYRSWTRARIIARIIELHQQNADLSWRNVSEKVDPQLAAAA
                     TKPRGFGSWQAAIEAAGLDYDAIRRYRRWDEARIVAGLRELAAQGVRLNSKEAQASYI
                     TLFAAAVRHFERWDRALEAAGLNARSIRMRAPFQRRRTHRRSLVELFERRQRARRCSK
                     "
     gene            complement(1830..2765)
                     /locus_tag="PLY56_07085"
     CDS             complement(1830..2765)
                     /locus_tag="PLY56_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HOJ21280.1"
                     /translation="MQINTRTLASRRLARVLALGAAVLWAAGCGGALDSVDGTGTPPP
                     PGGTELRATLRGTQEVPAVQTLGSGSASAILSADQSTLTVTLQTQGLTNVQLAHIHVA
                     PVGQNGPIILPLYDAATDGAFPATLSRVLTAADLQPQPAAGVTTFADAVQAILNGRTY
                     INVHTQQNPGGEIRGQLGPVSLRAVLSGDNEVPPVITTATGNADIVLNGLQSQVQVVL
                     TTSGLQNVVAAHIHVGAAGEEGGIILPLYDASIDGVFPAVLTRVVTAADLQAQPTGGI
                     VNFDDAINAILSGNTYVNVHTQQNPDGEIRGQITP"
     gene            complement(3315..4181)
                     /locus_tag="PLY56_07090"
     CDS             complement(3315..4181)
                     /locus_tag="PLY56_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ21281.1"
                     /translation="MQKRALGKGLDALFHSNPPEAEMAQGSVSMVPTASIRANPYQPR
                     GEMDPERLAELAESIRAHGVLQPLVVRPHKEGYELVAGERRFRASQAAGLEEVPVIIR
                     ECTNREMLELALIENIQREDINPIDAAIAFRRLIDEFGLTQEEVAARVGKSRPAVANT
                     LRLLNLPPAIRQRVASGELSEGHARALLSLESSPDAQLALADRIVAEHLTVRDTEKLV
                     RQCAEQKRPAESTTPAPTPRDPYLTKIEEHLQRYFGTRVSISRNGERGAIRIDFYDQE
                     QFERILELLTTT"
     gene            complement(4159..4935)
                     /locus_tag="PLY56_07095"
     CDS             complement(4159..4935)
                     /locus_tag="PLY56_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOJ21282.1"
                     /translation="MPRVIAVVNQKGGVGKTTTAVNLSACLASQSRRVLLIDSDPQGN
                     ATSGLGVGKAHLRQCIYDILINDAAITDVVLHTEVENLDLVPATINLAGAEIELVAEM
                     SREVRLRNALEAVLDDYDYILIDSPPSLGLITLNVLAAARSVLIPIQCEYYALEGITQ
                     LMNTVQMVRKHLNKSLALLGVVLTMHDFRTRISNEVIREVRGYFKGSVFQTIIPRNVK
                     LSEAPSHGQPIHIYDPKSRGAEAYAQLATEVMRIAEESSR"
BASE COUNT          849 a         1549 c         1632 g          926 t
ORIGIN      
        1 acacaccgcg gcgctgggga tcgcccttaa gcgcgggcga cttggacgtt ggctgaacct
       61 tgcgtcgccc tttccgtacg agctggctga tcgttggcat gaacttctct tctccctcag
      121 gtgcttctag tttcttcctg aacgcgccgg gctcgcgaac ctggcgcggc tttcctcgga
      181 acgctatcgg ttggccaacc ttttgcgtcc tgaagctgct ctcttggtgc gcccctcaac
      241 cgtccatcaa tcacgcgcaa gaggcggcac gaaaaaaccc ctcgggcatg ggaggggctg
      301 gcgttccacg tcacgtggtg gcggatcgtc cgacgttacc gctgtaggga aaccgactct
      361 tcctgtattc cagcccccta tcacgacgtg cccgccagcg cggacactat cgaaagcgcg
      421 tcacgcgtca caagggcacg tgggcgcgtt ggttgctctg tttggcaggc atactccgtg
      481 ttgacacttc acgaatgccc gagtgcagag tataacacgc gtgagagtat ttgtcaacaa
      541 gagatccttg cagaaagagc acattcgctt ggaatctctt ctcacgggag ggcatcgagc
      601 gcttccggcg ggtacacgcc ggggctgata ccgcggatga agggtacgaa cgcggatggg
      661 gaggtggggg gcgcctccgg atctcctacc cccagccccc tctctgtccg cccgccccac
      721 accaggcaca gggaaggaaa gcgaccgcat cgcgctcctg gagagcctcg aaggcctgcc
      781 caatacggac ccctgtgctt cctggcttcg ggagcgagag ccagacatgc ccccagaggc
      841 ggatcctggg cctttacatc aaagcgtgtg ctgtgctata attgcgcacc gcattcgtaa
      901 gcctcaacgt ttgctgtctg tctctgcttt gtgcggaaaa ggcgggtgac atgcgccggt
      961 ggaccagaga tgacatcgcg atggagatcc tgcagttgta cgcgtctggc ggggaactca
     1021 actacagcag catggctgag acgcaaccct cgctcctccg agcggcgacg cgccactttg
     1081 ggtcttggcg gagtgccgtg gagtatgcgg gcctttcgta cgatcagatt cggaaatacc
     1141 gatcctggac tcgggcacga atcatcgccc gaatcatcga actgcaccag cagaacgcag
     1201 atctgagctg gcgcaacgtc agtgagaaag tggatcccca actcgctgcc gcggctacaa
     1261 agcctcgggg ctttggaagc tggcaagccg ctatcgaggc ggccggcctc gattacgacg
     1321 cgatccgccg ctaccggcgt tgggatgagg cccggatcgt cgccgggctg cgggagctcg
     1381 ccgcccaggg agtccgcctc aactcgaagg aggcgcaggc cagctatatc accctcttcg
     1441 cggccgcggt ccgacatttc gagaggtggg accgcgccct agaggctgct ggcctaaacg
     1501 cgcgctcgat tcgaatgcgt gcccccttcc agcgccggcg aacacacagg cgcagcctcg
     1561 tggagttgtt cgagcgccgc cagagagcgc gccgttgctc caagtaatcc ggcggctctt
     1621 tctccggggt gccagcgtga gcctgcgctg gtccggtctg ggcatccatg caccccacgg
     1681 gctcgcacct ccgcgccggc cggctgattc ttttcctcta tattaatact acatatcgcg
     1741 ggggtgcatg acgagcgccc gcatatgttg ggatctagtg ggaggggagg ggggtgcatc
     1801 ctccggtggc cctcccctca agaatagcct tacggcgtga tttgcccgcg gatctcgcca
     1861 tcagggttct gctgggtatg cacgttcaca tacgtgttcc cgctcaggat ggcgttgatc
     1921 gcgtcgtcga agttgacgat cccgcccgtg ggctgcgcct ggagatcggc cgctgtgacc
     1981 actcgggtga gaaccgcggg aaacaccccg tcgatgctgg cgtcgtagag cggcaggatg
     2041 atcccgcctt cctcgccagc cgcgccaaca tggatatgcg ccgcgaccac attctggagc
     2101 ccggacgtcg tcaagaccac ctgcacttgc gactgcaagc cattgagcac gatatccgcg
     2161 ttgcccgtcg cggtggtgat aaccgggggc acttcgttat cgccagacag tacggcgcgt
     2221 agcgagacgg gtcccaactg cccgcggatc tcgccgccag gattctgctg ggtgtgcacg
     2281 ttgatgtagg ttcggccatt caggatcgcc tgcaccgcat cggcaaacgt ggtcacgccc
     2341 gctgccggct gaggctggag gtctgccgct gtcagcacgc ggctgagggt cgcgggaaac
     2401 gcgccatcag tcgccgcgtc gtacaggggc agaatgatgg gaccgttctg tcccaccggc
     2461 gccacatgaa tatgggccaa ctgaacattg gtcaagccct gggtttgcag ggtgaccgtg
     2521 agggtagact ggtcggcgct cagaatcgcg ctggccgacc cactgcccag ggtctgtacg
     2581 gcgggcactt cctgggttcc tcgaagcgtg gcccggagct ccgtgccgcc ggggggtggg
     2641 ggcgtaccgg tgccgtcgac tgagtcgagc gcaccgccgc agccggccgc ccacagcacc
     2701 gccgccccaa gcgccagcac gcgcgcgaga cgcctcgagg ctagtgttcg ggtgttgatc
     2761 tgcatcgagc cttctccttt cgcctcactc ctgccgccgg gcgctcattg ctgggtaccg
     2821 cggcgggcga ggggtctctg acaggatagg tcgcgcaagc ggatgcgggc caggtccatg
     2881 ccagaagccc cccctgtcat gcagctcttc ggagcaggtt ccgggccaga ttggggggcg
     2941 tggatggcgg tcggtttcag gcgccaggcg gtgcacctgc aagcgaccgg cggcacgggg
     3001 gcatatcctc ttcgcggagt ggccagcggt aagcactcgg ttcgacctac aggacgggga
     3061 acgagctcgc caccgtgccg cgccactctc ccggcaggag tgttcgtact cctggcggag
     3121 caggggatcc gtgccgaggc ccagtccaca agaccacgat cactttcggc cgtgtgacca
     3181 gcaggcaggg tggattatcg ggtgccggct ggccgccaga cgcgaggaag cacgccaacg
     3241 cgaccgggca cgaagcgatg cccggtctca tcgcgcgcca ggggcggccc ctgaaaccgt
     3301 gggtagtcga ggcgttacgt cgtggtgagg agctccagga ttcgctcgaa ctgctcctgg
     3361 tcgtagaaat cgatgcgaat cgcaccccgc tcgccgttgc gcgagatgga gacgcgggtg
     3421 ccgaagtagc gctggagatg ctcctcaatc ttcgtgaggt agggatcccg cggggttggg
     3481 gcgggagtcg tgctctcggc aggtcgcttc tgctccgcac actgccggac cagcttctcg
     3541 gtgtcgcgca cggtgaggtg ctccgcgacg atgcggtcgg cgagggcgag ttgtgcatcc
     3601 ggtgaggact cgaggctcag gagggcgcgt gcgtggcctt cggagagctc gccagaagcc
     3661 acccgctgcc ggatagcggg cgggaggtta aggaggcgga gggtgttggc caccgctgga
     3721 cgtgatttgc ccacgcgagc cgcgacttcc tcctgggtga gcccgaactc atcgatcagc
     3781 cggcgaaagg cgatggcggc atcgatggga ttgatatcct cgcgctggat attctcgatg
     3841 agcgccagct cgagcatctc ccggttggtg cactcgcgga tgatgaccgg cacctcttcc
     3901 agaccagcag cctgcgatgc ccggaagcga cgctcgccgg ccacgagctc gtagccctct
     3961 ttgtgcgggc gcacgacaag cggctgcaga acgccatggg cgcggatcga ctccgccagt
     4021 tctgccaggc gctccgggtc catctcgccg cgaggctgat atggatttgc ccgtatgctg
     4081 gcggtcggca ccatagacac gctcccctga gccatctccg cctcgggagg gttgctgtgg
     4141 aacagggcat ccaggccttt accgagagct ctcttctgca attcgcatta cctccgtggc
     4201 gagctgcgcg tacgcttcgg ccccacgaga cttggggtcg tagatatgga tcggctggcc
     4261 gtggctgggc gcctcgctca gcttgacgtt ccgggggatg atcgtctgga agacgctccc
     4321 cttgaagtag ccccgcacct cgcggatcac ctcgttggag attcgggtgc ggaagtcgtg
     4381 catggtcagg acgacgccga ggagcgccag gctcttgttc aggtgcttcc gaaccatctg
     4441 caccgtgttc atgagctggg tgatcccctc gagcgcgtag tactcgcact ggatcgggat
     4501 gagcacggat cgcgccgcgg cgagcacatt gagcgtgatc aggccaagcg agggcgggga
     4561 gtcgatcaga atgtagtcgt agtcatccag gaccgcctca agcgcgttgc gaagacgcac
     4621 ctcgcgcgac atctcagcga ccaactcgat ctccgcgccc gcgaggttga tcgtcgccgg
     4681 cacaagatcc aggttctcca cctcggtgtg caacaccacg tcggtgatag ccgcgtcatt
     4741 aatcaggata tcgtagatgc attgacggag gtgcgccttc cccacgccga gcccgctggt
     4801 agcgtttccc tgcgggtcac tgtcgatgag gaggacacgc cgtgactggg atgcaaggca
     4861 tgcggaaaga ttgacggcgg tggtggtctt gccgaccccg cccttctggt tgactacggc
     4921 gataactcgt ggcatattcc gtccacgggg cggttt
//
LOCUS       DAOSCV010000233         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10755_length_4942_cov_3.843667, whole genome shotgun sequence.
ACCESSION   DAOSCV010000233 DAOSCV010000000
VERSION     DAOSCV010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10755_length_4942_cov_3.843667"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLY56_07100"
     CDS             <1..377
                     /locus_tag="PLY56_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOJ21283.1"
                     /translation="DERSEAYPFEVSAEGDARVLDLRPAIREIVHARRAGEPVSLLAA
                     RFHATVAEGIVQLCRWVAEETGLRCVCLSGGTFQNVRLLEAVVTGLENSGLQVYWHRV
                     VPTNDGGLSLGQAVVAGAILAT"
     gene            428..670
                     /locus_tag="PLY56_07105"
     CDS             428..670
                     /locus_tag="PLY56_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOJ21284.1"
                     /translation="MCLAIPMQVKAVEGSEAVVTQGGTELRVRTDLLDAVEVSDYVLV
                     HAGFALQVVDPEEARATLAMLREMAGDAPQEGEARG"
     gene            663..1784
                     /gene="hypD"
                     /locus_tag="PLY56_07110"
     CDS             663..1784
                     /gene="hypD"
                     /locus_tag="PLY56_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOJ21285.1"
                     /translation="MAETGPDGAMRMMDEFRAAGAVEALRAAIAAAVPRPVKLMEVCG
                     THTMAIARAGLRGLLPQEITLLSGPGCPVCVTAQGEIDAFLALGERPNVVLTCFGDMM
                     RVPGSAGSLEQMRARGTEARVVYSPMDALVLAIEQPSKEVVFFGVGFETTVPTVAATL
                     KEASRRGVRNFSVLAAHKTIPGALAALASAPDLAVDGFLLPGHVSVILGEAPYRFLAE
                     EHGVACAIAGFEATDILDGIRALVEQIRDARPTVANRYHRAVRSEGNPTARALVDSVF
                     APVDATWRGIGLIPGTGLAIREEYAPWDARARFADLDWERFANVPEPRGCRCGEILKG
                     ALTPRDCPLFGRACTPARPVGPCMVSSEGTCAAYYRYQL"
     gene            1845..2849
                     /gene="hypE"
                     /locus_tag="PLY56_07115"
     CDS             1845..2849
                     /gene="hypE"
                     /locus_tag="PLY56_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOJ21286.1"
                     /translation="MSIVLLGHGGGGRLMAELIEGLFLPRLGSPDLARADDSAVLPEP
                     GGQIAFTTDSFVVDPPIFPGGDIGRLAVCGTVNDLAASGARPLYLSTAFILEEGLPLE
                     TLERVANSMAAAAEEAGVRLVAGDTKVVPRGKADRLFITTSGIGVIPEGMNVSGHHAR
                     PGDVVLCSGTLGDHGMAIMVTREKLGFAGPIESDVAPLNGLVEALHPIRHGLHALRDA
                     TRGGAAAVLNEIASQSGVAIEVAEEAVPVRPAVRAACELLGIDPLYVANEGKLIAVVA
                     PEVAEEALAIWRAHPLGRDAVRLGEVLESPAGRVHARTPFGTTRLLDRPLGEQLPRIC
                     "
     gene            complement(2851..>4942)
                     /locus_tag="PLY56_07120"
     CDS             complement(2851..>4942)
                     /locus_tag="PLY56_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ21287.1"
                     /translation="EGGTNLLLRLRYRVAGQPESVELHVRPLDDKGQVLLPVEEYPQV
                     RARLSMAEGWSDFSRVCYFPPQVAAYDITLRLQGEGTLAIDAIEGQALRPEEFAVPSL
                     GARIKGACSEAVTVWYESSLKKVYRDARPPEVETPAVEITAARGETEAFQIVLLPRED
                     AEGVRVRWQDLLGPGVLHREHGRVAWVEYLHVTRPRAPLARAGWIPDPLLPDDTRDLR
                     GGQVQPVWLSFTVPKDARPGLYRGAVQIEPAEREDYYGYYSRPSLLVTRIPVKLRVLS
                     YALPEEPALTTMARITRCPPEGREAFRENFRAHRVTGEAYAGPLPARVSPDGNVALEF
                     APFDEAVERYLAKGLLLLNFPGTFLGDARGFFEEDGRWHGLELFSPGFNQAFTSYVYQ
                     VARHLREKGWLRHAILQLWDEPTGEAREMHRRLASLVRAAAPDARVCLATPPDSELLD
                     DVDIWNVPLPDGYEESAARAFRERGGELWGYDNRLYSLDVESSSIEMRAYPWRLYRYG
                     FSGAEWWSVSEWLGDPYTEPDRCGAQNGGGFLLYPPRDGAVAPVNSIRWELYREGVED
                     YDALALLAEKYTAARESLGVPDGSFDVETLLARITGRVARSIADATRDPRDVIAAREL
                     VDRLIEMASASSPCVVRFKEDRKGVFLEGLTLPRAQVSFGGVKARANAAGRVRFALPD
                     EWRLPTGAGASPRN"
BASE COUNT          751 a         1668 c         1737 g          786 t
ORIGIN      
        1 gcgacgagcg cagcgaggcg tatccctttg aagtgagcgc ggaaggcgat gcgcgtgtcc
       61 tggatctgcg cccggccatt cgggagattg tccacgcccg gcgcgcgggt gagccggtct
      121 cgcttctcgc ggcgcggttc cacgccacgg tggccgaggg gatcgtgcag ctctgccgct
      181 gggtggcgga agagacgggc ctgcgctgcg tctgcctgag cgggggcacc ttccagaacg
      241 tccggcttct cgaggcggtg gtgaccggcc tggaaaactc gggattgcag gtgtattggc
      301 accgcgtggt gccgacaaat gacggcgggc tctcgcttgg ccaggcggtc gttgccggcg
      361 cgatcttggc aacgtaggcc gagcctatgc tgcccacgtc ttacgttaga gatggcagga
      421 taaccgtatg tgtctggcga taccgatgca ggtgaaggcg gtagagggct ctgaggcggt
      481 cgttacccag ggcggcacgg agctgcgcgt gcgcaccgac cttctggacg ccgtggaggt
      541 ctccgattac gtcctggtac acgccggctt cgcgcttcag gtggtggacc cggaagaggc
      601 gagggcgacg ctggcgatgc tccgggagat ggccggggat gcaccccagg agggagaggc
      661 gcgtggctga gacggggccg gacggcgcaa tgcgcatgat ggacgagttc cgcgccgccg
      721 gcgcggtgga agcgctgcgc gcggcgatag ccgctgccgt gcctcgcccg gtgaagctga
      781 tggaggtgtg cggcacgcac acgatggcaa tcgcgcgcgc cggcctgcgc ggcctgctgc
      841 cccaggagat cacgctcctc tctggcccgg gctgccccgt ctgcgtcacc gcgcagggcg
      901 agatcgacgc cttcctcgcc ttgggcgagc gcccgaacgt ggtcctcacc tgcttcggcg
      961 acatgatgcg cgtgcccgga agtgccggca gcctggagca gatgcgcgcc cgcggcaccg
     1021 aggcgcgcgt cgtctactcg cccatggatg cgctcgtcct ggccatcgaa cagccttcaa
     1081 aggaggtggt cttcttcgga gtgggcttcg agacgacggt accgaccgtc gccgccactt
     1141 tgaaggaggc aagccggcgc ggcgtgcgca acttctcggt tctggccgcg cacaagacga
     1201 tcccgggggc gctggctgcg ctcgcctcgg cccccgatct cgccgtggat ggcttcctcc
     1261 tccccggaca cgtgagcgtg atcctgggtg aggcgccgta tcgcttcctc gccgaggaac
     1321 acggcgttgc ctgcgcgatt gccggcttcg aggcgacgga tattctggat ggcattcggg
     1381 cgctggtgga gcagattcgc gatgcccggc ctacggtcgc gaaccgctac catcgcgcgg
     1441 tccgctcgga gggcaacccc acggcgcgcg ccctggtcga ttcggtcttc gcgcccgtcg
     1501 atgccacctg gcgcggcatc gggctcatcc cgggtaccgg cctggcaatc cgtgaggagt
     1561 acgcgccgtg ggacgcgcgc gctcgcttcg ccgacctcga ttgggagcgc ttcgccaacg
     1621 tgcccgaacc ccgaggctgc cgctgcggcg agattctgaa gggcgccctg acgccgcgcg
     1681 actgccccct cttcggacgc gcgtgcacgc cggcgcgccc tgtcggtccg tgcatggtct
     1741 cgagcgaggg cacctgcgcg gcctattacc ggtatcagct ctgagcgcgg gccgcttcgg
     1801 tccaggaacc gcgctttcga gaagcgggaa ggaaaccgtg acacatgagc atcgtgctgc
     1861 taggtcatgg aggcggcggt cgcctgatgg cggaacttat tgagggcctc tttctgcccc
     1921 ggctggggag cccggatctc gcgcgcgccg atgatagcgc cgttctcccg gagccgggcg
     1981 ggcagatcgc cttcacgacc gactccttcg tcgtcgaccc gccgatcttt ccgggaggag
     2041 acatcgggcg tttggccgtc tgcggcacgg tgaacgacct ggccgcgagc ggcgcgcgtc
     2101 cgctttacct gagcacggcg ttcatcctgg aggaggggct tccgctggag accctcgaac
     2161 gtgtcgccaa ttccatggcg gctgcggcag aagaggccgg agtgcgtctc gtcgccggag
     2221 atactaaagt ggtcccacgc ggcaaggcag accggctttt catcaccacg tccgggatcg
     2281 gcgtcattcc cgaggggatg aacgtctccg ggcaccatgc caggccgggc gatgtggtcc
     2341 tctgtagcgg cactctgggt gaccacggca tggcgatcat ggtcacgcgc gagaaactcg
     2401 gcttcgccgg gccgattgag agtgacgttg cgccacttaa cggcctggta gaggcgctcc
     2461 acccgatccg gcacgggctg cacgccctac gcgacgcgac gcgcggcggt gccgcggcgg
     2521 tgctgaacga gatcgcttct cagtctggag tagccatcga agtggccgaa gaggcggttc
     2581 cggtgcgccc cgcagtgaga gcggcgtgcg agctcctcgg catcgacccg ctttacgtgg
     2641 caaacgaggg gaagctgatc gccgtcgtcg cgcccgaggt cgccgaggag gcgctggcga
     2701 tctggcgcgc ccacccgctg ggccgcgacg ccgttcgcct gggtgaggtg ttggaaagcc
     2761 ccgccggccg ggttcacgcc cgcaccccct ttggcaccac gcgcctgctt gatcgcccgc
     2821 tgggcgagca gctcccgcgg atctgctgag ctagttccgc ggcgaagcgc ccgcccctgt
     2881 cggcagccgc cattcatccg ggagagcgaa gcgcacccgt ccggcggcat tcgcgcgcgc
     2941 cttcaccccg ccgaaagaga cctgggcacg cgggagagtg aggccttcga ggaagactcc
     3001 tttgcgatcc tccttgaacc ggaccacgca cggggaagag gcactcgcca tctcgatcag
     3061 gcggtcgacc agctcgcgcg ccgcgatcac atcgcgcggg tcgcgcgtgg catcggcgat
     3121 ggagcgggcc actcgcccgg tgatgcgggc caggagggtc tccacgtcaa agctcccgtc
     3181 cggcacgccg aggctctcgc gggcggcggt gtacttttcc gccagaaggg ccagggcgtc
     3241 gtagtcctcc acgccttcgc gatacagctc ccagcggatg ctgttcactg gtgcaaccgc
     3301 gccatcgcgc ggcgggtaga gaagaaagcc gccgccgttc tgggcgccgc accggtccgg
     3361 ctccgtgtag ggatcgccca gccactcgct gaccgaccac cactccgcgc cgctgaagcc
     3421 ataccggtag agcctccagg ggtaggcgcg catctcgatg gacgagctct ccacgtccag
     3481 gctgtaaagc cggttatcat agccccacag ctccccgcct cgctcgcgaa acgcgcgcgc
     3541 ggcggattcc tcgtaaccat caggcagggg cacgttccag atgtcgacat cgtcgaggag
     3601 ctcgctatcc gggggcgtgg ccaggcaaac gcgggcgtcc ggcgcggcgg cacgcacgag
     3661 cgaggcaagc cgacgatgca tctcgcgcgc ctcgccagtg ggctcgtccc atagctgcag
     3721 aatggcgtgg cgcaaccacc ccttctcgcg gaggtgacgc gctacctgat acacgtaact
     3781 cgtgaacgcc tggttgaagc ccggtgagaa gagctccagg ccatgccacc ggccgtcttc
     3841 ttcgaagaag ccgcgcgcgt ctcccaggaa ggtgcccggg aagttgagga ggagcaaccc
     3901 tttcgccagg taacgctcca cggcctcgtc aaacggcgca aactccagcg caacgtttcc
     3961 atcgggcgac acgcgcgcag gcagaggccc ggcgtatgcc tcgccggtga cgcgatgggc
     4021 ccggaagttt tcgcgaaacg cctcgcgccc ttcgggcggg caccgggtga tccgcgccat
     4081 cgtggtgagc gccggctctt ccgggagcgc gtagctgagc acgcgcagct tcacgggaat
     4141 gcgcgtcacc agcaggctgg ggcgggagta gtagccgtaa taatcctctc gctccgcggg
     4201 ttcaatctgc accgcgccgc ggtagagccc cgggcgcgcg tctttgggca cggtgaagct
     4261 gagccagacc ggctggacct gcccgccccg gagatcccgc gtgtcgtccg gcaagagagg
     4321 gtcggggatc cacccggcgc gcgcgagcgg ggcgcgcggg cgcgtgacgt gaagatactc
     4381 cacccaggcc acccggccat gttcccggtg gaggacaccc ggaccgagga ggtcttgcca
     4441 gcgcacgcgc acgccttcgg cgtcctcgcg agggaggagc acgatctgaa acgcctcggt
     4501 ctcgccgcgc gccgcggtga tctcaacggc gggagtctcc acctcgggcg gcctggcatc
     4561 gcgatatacc tttttgagcg agctctcgta ccagacggtg accgcctccg agcaggcgcc
     4621 cttgatccgt gccccgagcg agggcacggc gaactcctcc gggcgcagcg cctgcccctc
     4681 gatcgcgtcg atggcgagcg tgccttcgcc ctgcaggcgg agggtgatat cgtaggcggc
     4741 tacctgcggc gggaagtagc aaacgcgcga gaagtcgctc cacccctcag ccatcgacag
     4801 gcgcgcgcgc acctgggggt actcctccac cgggagcagc acctggcctt tatcgtccag
     4861 cgggcgtacg tggagctcga cgctctccgg ctggccggcc acccggtagc gcaggcggag
     4921 gaggaggttc gtcccgcctt ct
//
LOCUS       DAOSCV010000234         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10770_length_4938_cov_3.437641, whole genome shotgun sequence.
ACCESSION   DAOSCV010000234 DAOSCV010000000
VERSION     DAOSCV010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10770_length_4938_cov_3.437641"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLY56_07125"
     CDS             complement(<1..498)
                     /locus_tag="PLY56_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ21288.1"
                     /translation="MMDVSHLIRTPEEIDRLFPWLREERVRGIPHFISPAWNTDRSHF
                     DPEYYAECWERLGFRSVTLLTGHHDGCLLYPSRLSHQQPGRDYFGEQVAACRKRGIRV
                     LAYYSLSLDSLVGSEHPEWRIRDLAGRAHPVDYRGFSHYHWLCLNSPYRDFAIQQLQE
                     VVQNYP"
     gene            complement(495..1295)
                     /locus_tag="PLY56_07130"
     CDS             complement(495..1295)
                     /locus_tag="PLY56_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21289.1"
                     /translation="MKVVISALHFPWSDMKACLRHATAGLGLDGVELSWHSSFARPHC
                     TREDIEQLVRLAPGSGIELCAHIWENLARMEPGSAEDALRGWLELCGRTGVTGLVIHG
                     GSYDDQREGIARTRRLLERVLPQFERQGVTLLLENHYAYDYRNCHELFSEPWEFQEVL
                     SLESPALRICFDTGHAHMTRNIDELLTSLSDHLQHIHLADNDGRDDLHCAYAAGTVPF
                     ERIYDTLATLGFDGTFCIESPVRDDLRSFRKCLSMIRERWPGAAGRGR"
     gene            1647..3143
                     /locus_tag="PLY56_07135"
     CDS             1647..3143
                     /locus_tag="PLY56_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOJ21290.1"
                     /translation="MAHRVVRALACITFLLLFPAASEAAPAVTAARVLRVADDGTLAL
                     DTGEHLRLLGTDFPHDPVAALKSRRILESMVGGKNVRVVTGEPREDAQGVSLAYIYLE
                     DGALVNARLIKQGYAFAAAGYPFAKREEFRQYQRQARDAGAGVWAKPKEIVHRASDPA
                     PSPGRMPAPASRASRSGSGFRLPVSESVGGRLLAATVIGFLSFYLLVYLVLLWKSEAR
                     ADRLLAQLAMSGLGAVGLTLLMPLVLLLLSESFEPTGGSWAAGVLTALGVLSGRSAAR
                     AWQRGQLLRGTPTTRLRSTSHGFCKARGFTYPAYGILRSGIGGIPCLYYSEVTEEYQR
                     HTETYYDAQAKKTRTRVVYRWVPIHSATRAVNFGLNDNTATATVVVDGAQFFPAHRAF
                     LYNNRPAGHLPWTARVGDIRTTVEYIAPETALTVWGRYVETGRESVDQVDRQIRYDEH
                     HGCLVICEGNESRVYGHHTGAGLGLALLTAIIFLGVILLIAVPVATGF"
     gene            3162..3725
                     /locus_tag="PLY56_07140"
     CDS             3162..3725
                     /locus_tag="PLY56_07140"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ21291.1"
                     /translation="MDTLSTIAVVGFVLGCIVVIWLISTYNSLVNLNGQVKRAWANVD
                     VVLKQRWDLVPNLVETVKGYARHEQEVFGRIAELRAQALGAPNRKQRINAEQQLDRLM
                     PRVVAWLEQYPMLRASENFGQLQAELSRLEEQIADRRELYNEAATHYNVYRASFPALL
                     LAQSLGSEEAPLFEIVAQEREAPRVKF"
     gene            complement(4286..>4938)
                     /locus_tag="PLY56_07145"
     CDS             complement(4286..>4938)
                     /locus_tag="PLY56_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ21292.1"
                     /translation="MIGAMLGVRESAHFIRGLENLWADIYEHPDELRRLLDILVEMNL
                     YAIQRYAKADVDGFMFCDDWGLQERLMISPDKWREFWKPCYARVYAAAREAGMLTFLH
                     SCGYIVDILDDLIEAGLDVIQMDQQENMGLENLGERFGGRITFYCPVDIQKTMVYGSL
                     DDIRAYCRRMVRALGRPEGGFIPKWYGDPKGAGHTQEAIDAMCTEFLRISDEMYSR"
BASE COUNT          826 a         1522 c         1750 g          840 t
ORIGIN      
        1 cgggtagttc tgcaccacct cctggagctg ctggatggcg aaatcgcggt aggggctgtt
       61 gaggcagagc cagtggtagt gcgagaagcc gcggtaatcc acgggatggg cgcgcccggc
      121 gagatcgcgg atccgccact ccgggtgctc gcttcccacc aggctgtcga gtgagagcga
      181 gtagtaggcg aggacgcgga tcccgcgctt gcggcacgca gccacctgct cgccgaagta
      241 atcgcggccc ggctgctggt gcgagagacg ggaggggtag aggagacagc cgtcgtgatg
      301 ccccgtgagc agggtgacgg agcgaaaccc gagccgctcc cagcattcgg cgtagtactc
      361 tgggtcgaaa tgcgagcgat ccgtgttcca cgcaggcgag atgaaatgcg ggatcccgcg
      421 cacccgctcc tcacgcagcc acgggaaaag ccggtcgatc tcctcggggg tgcgtatcag
      481 gtggctgacg tccatcatct ccctcttcct gcggcgccgg gccagcgctc gcggatcatc
      541 gaaaggcact tccggaagga gcgcaggtcg tcgcgcaccg gagactcgat gcagaaggtt
      601 ccatcgaagc cgagcgtggc gagcgtgtcg tagatccgct cgaacggcac cgtgccggcg
      661 gcatacgcgc agtgcaggtc gtcgcggcca tcgttgtcgg ccaggtggat gtgctgcagg
      721 tggtcgctca ggctggtcag gagctcgtcg atgttgcgcg tcatgtgggc gtgcccggta
      781 tcgaagcaga tgcgcagcgc cgggctctcc agcgagagga cctcttgaaa ctcccacggc
      841 tcgctgaaca gctcgtgaca gttgcggtag tcgtaggcgt agtggttctc caggaggagc
      901 gtgacgcctt gccgctcgaa ctggggcagg actcgctcca ggaggcggcg cgtccgcgcg
      961 atcccttccc gctggtcgtc ataactgccg ccgtggatga ccaggccggt gacgccagtt
     1021 cgcccgcaaa gctcgagcca gccccgaagc gcgtcctcgg cggagccagg ctccatgcgc
     1081 gccaggtttt cccagatgtg cgcgcacagc tcgatgccgc tgccgggcgc cagccggacg
     1141 agctgctcga tatcttcgcg cgtgcagtgg gggcgggcaa aggatgaatg ccacgagagc
     1201 tccacaccgt ccaggcccag gcccgcggtc gcgtgccgga ggcacgcctt catatcactc
     1261 caggggaagt ggagcgcgct gattacgacc ttcatcgctg cctctatctt tcaaagcggt
     1321 gggacgatcc gttgcgcggt tccatctcgc tcccggcgct tctggagccg ggatcaccct
     1381 ctcgttcgac ggggaggcgg ccactcctgc ggcgcgtctc ggaaagagtg ctcgcccgcc
     1441 gggccggtgg caggaggacg gcgagcgcgt gtagaagcag ccagacggcg gcgcgcgctt
     1501 gagggccgac aaatgctgcc cgcggggagt gttacggggt ggcgtaaaga agggtgcaga
     1561 gcgggaaacg caccgcgctc ctgtcgggga gcaccggccg gcgggagcgc ggcgcgcttc
     1621 gcgacgggtt gggagacagc cgggagatgg cacaccgggt agtgagagcg ctggcatgta
     1681 tcacgttcct cctgctattc cctgctgcct cagaggcggc accagcggtg acggcggcgc
     1741 gggttctccg cgtggcggat gatggcacgc tcgcgctcga taccggggag catctccgcc
     1801 tcctgggcac cgactttccc catgacccag tggccgcctt gaaaagcaga cgcatcctgg
     1861 aaagcatggt gggcggcaag aacgtgcgcg tggtgaccgg tgagccgcgc gaggatgctc
     1921 aaggcgtgag tctggcgtat atctatctgg aggatggcgc gctggtcaac gcccgactga
     1981 tcaagcaagg ctacgccttc gccgccgcgg gctacccctt cgcaaaacgc gaggagttcc
     2041 ggcagtatca acgccaggcg cgtgacgccg gggccggtgt gtgggcaaag cccaaggaaa
     2101 ttgtgcatcg cgcgtcagat ccggcgccct cgccgggccg gatgccggcg ccggcctctc
     2161 gcgcgtctcg gagcggctcc gggttccgat tgccggtttc ggagagcgtg ggcgggcgcc
     2221 ttctggcggc gaccgttata ggcttcctct ctttctacct cctggtttat ctcgtgctcc
     2281 tctggaagag tgaggcgcgc gccgaccggc ttctcgcgca gctggcgatg agcggcctgg
     2341 gcgccgtggg actcacgctg ttgatgcctc tggtgctcct actcctgagc gagagtttcg
     2401 aaccgacggg cgggagctgg gcagcaggcg tgctcaccgc gctgggcgtt ctctccgggc
     2461 gttcagcggc acgggcgtgg cagcggggcc agctgttgcg cgggacccca acgacgcgcc
     2521 tacggagcac atcgcacggc ttctgcaagg cgcgaggctt tacgtaccct gcctacggca
     2581 tcctccgcag tggcatcggc gggatcccgt gcctctacta ctcggaagtg actgaggagt
     2641 accaacgcca taccgaaacc tactacgacg cgcaggcgaa gaagacgcga acgcgcgtcg
     2701 tctaccggtg ggtgcccatc cacagcgcca cgcgcgcggt caacttcggg ctgaacgaca
     2761 acactgcgac ggcgaccgtt gtggtggacg gggcgcaatt ctttccggcg caccgagcgt
     2821 tcttatacaa caaccgcccg gcggggcacc tgccatggac cgcacgggtg ggcgatatcc
     2881 gtacgacggt ggagtatatc gccccagaga cggcgctcac cgtgtgggga cgctatgtgg
     2941 agacgggccg ggagagcgtg gaccaggtgg atcgccagat ccgctacgat gagcaccacg
     3001 gctgcctggt catctgcgaa gggaatgagt ctcgggtgta cgggcaccac actggcgctg
     3061 ggttgggatt ggcgctgctg acggcaatca tctttctggg ggtcatcctg cttatcgctg
     3121 tgcccgtggc gacgggcttc tgaggggaag ggtggtatcg gatggatacg ctttcgacga
     3181 tcgcggtcgt ggggtttgtg ctcggttgta tcgtggtgat ctggctgatt tccacttaca
     3241 actcgctcgt gaatctgaat ggacaggtga agcgcgcgtg ggccaacgtc gatgtggttc
     3301 tgaagcagcg ttgggacctg gtgccgaacc tcgtggagac ggtgaagggc tacgcgcggc
     3361 acgagcagga ggtgttcggg cgcatcgccg agctccgcgc ccaggcgttg ggcgcgccga
     3421 accggaagca gcggatcaac gccgagcagc agttggatcg gctcatgccg cgggtggtcg
     3481 cctggctcga gcagtacccg atgcttcggg cgagcgagaa cttcggccag ctgcaggccg
     3541 agctgagccg cctggaagag cagatcgccg accggcgcga gctgtataac gaggcggcga
     3601 cgcactacaa cgtgtaccgg gcgtccttcc cggcgcttct cctggcgcag agcctcggca
     3661 gcgaggaagc cccgctcttc gagatcgtgg cccaggagcg cgaggccccc cgcgtcaagt
     3721 tctaagccgg ctggtcgcac ggacctaaag ctccgcgctc cttgtgggcg aagccctgcc
     3781 gggctgggcc gggcggcatt cccgggtgcc tcaagaaggc gagggttggg agggggagcc
     3841 cgaagggctt tgctgaaaag cagcgcggag ctttgagctc cgcgcgtgag tgagcaccga
     3901 ccccgcttat gtagcacgga tctaaagctc cgcgcttctt gcgagcgaag ccctgccggg
     3961 ctgggccggg cgacattctc gggtgcctca agaaggcgag ggttgggagg gggagcccga
     4021 agggctttgc tgagaagcag cgcggagctt tgagcaccgc gcgtgagtga gcaccgaccc
     4081 cgcttgtgta gcgcggacct aaagctccgc gctccttgtg ggcgaagccc tgccgggctg
     4141 ggcaactcgc ctgcgcaacg cagaggggcg ggagctgggc gtgggatgcc cgaagggctt
     4201 tgctgagaag cagcgcggag ctttgagctc cgcgcgcgag tgagcatcgg cccctcatcc
     4261 gtggtatcgc tgcccggccc ggcgcttagc gggagtacat ctcgtcgctg atgcgcagga
     4321 actcggtgca catcgcgtcg atcgcctcct gagtgtgccc ggcgcccttc gggtcgccat
     4381 accacttggg gatgaacccg ccttcaggcc gaccgagcgc acggaccatg cgccggcagt
     4441 aggcgcggat gtcgtcaagg ctgccgtaga ccatcgtctt ctggatgtcg acggggcagt
     4501 agaaggtgat ccgcccgccg aagcgctcgc cgaggttttc gagccccata ttctcctgct
     4561 ggtccatctg gatcacgtcg aggccggcct cgatcaggtc gtcgaggatg tccacgatat
     4621 agccacagga gtggaggaag gtgagcatgc ccgcctcgcg cgcggcggcg taaacgcgcg
     4681 cgtagcacgg cttccagaac tcgcgccact tgtcgggcga gatcatcagc cgctcctgca
     4741 ggccccaatc gtcgcagaac atgaagccgt cgacgtcggc cttggcgtag cgctggatcg
     4801 cgtagaggtt catctctacc aggatgtcca gcagccggcg cagctcgtcc gggtgctcat
     4861 agatgtcggc ccataggttc tcaaggccgc ggatgaaatg ggcgctctca cgcacaccga
     4921 gcatcgcgcc gatcatca
//
LOCUS       DAOSCV010000235         4936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10779_length_4936_cov_2.833436, whole genome shotgun sequence.
ACCESSION   DAOSCV010000235 DAOSCV010000000
VERSION     DAOSCV010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10779_length_4936_cov_2.833436"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..972
                     /locus_tag="PLY56_07150"
     CDS             340..972
                     /locus_tag="PLY56_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ21293.1"
                     /translation="MERYRLDAAPRTLLGKQKVKRLRAAGYYPAIMYGRGMEPTPLQV
                     SQEAFNKMLKTVGANTLVDLYIEGTDGPQTVLVKKLMRDSLSMQLLNIDFHRVSVTDV
                     ITNRIPVELVGEPRGVSEGGTLMQPLIDVEVRGVAASLPASLQVDVSHLGIDESLHVS
                     EISVPEGVEIVAAPDEVVAVVHAPRAAVEEPVEPEQITEPELVVKKEEEE"
     gene            complement(1038..2048)
                     /locus_tag="PLY56_07155"
     CDS             complement(1038..2048)
                     /locus_tag="PLY56_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21294.1"
                     /translation="MSTLGAAVIGARMGSGHVLGYVNNPHTELRAVCDTDLESARRVA
                     EKHSVPLVTDNWEELLERDDIQIISVATPDPLHEPMTVRSLEAGKHVLCEKPMALTVP
                     ECERMIAAADRTGKLLMVGQVCRFAPGFALAKRLVDRGEIGDLFFVESEYAHDYTHVG
                     GVGGWRKDPKVKREPFVGGGCHAVDLLRWVAGNMVECSAYSNHKVLTDWPVDDCTIAI
                     YKFESGVIGKVMCSIGCKRPYTMRSVFYGTRGTIICDNTSPAIQLYTKDLPESNGWIS
                     LPVNLASHNVTAEVNELVECILEGKPLLTDGREGARTVAACRAAVESAATGKPVAVRT
                     QF"
     gene            2289..2825
                     /locus_tag="PLY56_07160"
     CDS             2289..2825
                     /locus_tag="PLY56_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOJ21295.1"
                     /translation="MIKLRVVMAALAVLVLAACAPPASAQEQGRLAITGGLSWFTGDV
                     NDDNTWIAGLQYSFPTMTGQRSRGTFLLSADFLQVDSTRLDAGIPVTDHVNLIPVLLN
                     YRHSLGATPWYIGAGAGIFLSEENIPEMGISSDVNFAWQLFAGYNLGNNWFVEGRWLA
                     GNDPGDDGMLIAQLGFRF"
     gene            3372..4514
                     /locus_tag="PLY56_07165"
     CDS             3372..4514
                     /locus_tag="PLY56_07165"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOJ21296.1"
                     /translation="MRIVIAPDSFKGSLSAQEVCDAIARGVLRAAPETVIDTVPMADG
                     GEGTVQSLVDATGGELVMQHVTGPLGEPVTATFGLLGDGETAVIEMASASGLPLVPEG
                     RRNPLVTTTRGTGELMRAALDRGRRKLLIGIGGSATNDGGAGMAQALGAALLDGNGRP
                     IGPGGGALASLARIDLSGMDPRLAETEVVVACDVENPLTGARGASAVYGPQKGATPEM
                     VRQLDANLRHYAEIIRRDLGKDVAEVPGAGAAGGLGAGLIAFTGASLRRGVEIVIEAV
                     RLRERLRGADLVITGAGRTDGQTVFGKTPAGVARVAGELGIPVVGLVGSYSSDAGVVH
                     AHGIDALVSILHEPITVAEAIAQGATLIERAAEELMRLLLVGQRLR"
     gene            4701..>4936
                     /locus_tag="PLY56_07170"
     CDS             4701..>4936
                     /locus_tag="PLY56_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21297.1"
                     /translation="MLALRISTAVLVTLLAAGPALAAEAAKRLPWQREVDQPDPTAVK
                     PLPDLVSERWRSTGGAEGSVAPSAENGPWGAPYLR"
BASE COUNT          834 a         1632 c         1597 g          873 t
ORIGIN      
        1 gccggcttca atgccacgga tggaggagac ggagcgggct ccgacaacac gcgcttgaac
       61 gatgcgcccc ttcgatgtcg aggcgtatcc aacggggcgc tggcgggagg acccttcgat
      121 accggtttga cagcaacgcc ctgattgtgc tatactgcgc gcgtcattgg cactcccgcg
      181 gcggcgcacg gcgactacgg gggagaggca tcccgctcga atgtggcgcc tcaacctctc
      241 tggcgccggc cccacccttc acctttgttg ataccgcgag tgtcgcgcct ctggcgcgac
      301 cgacgccgcg gggaagcccc ggagaggagt attgtaccag tggaacgcta tcgtttggat
      361 gccgcgccgc gcaccctgct tggcaaacag aaagtgaagc gcctgcgagc cgccggctat
      421 taccccgcaa tcatgtatgg acgcggcatg gagcccaccc cgcttcaggt cagccaggag
      481 gccttcaaca agatgctgaa gacggttggg gccaacacct tggtcgatct ctacatcgag
      541 ggcaccgacg gcccccaaac cgtgttggtg aagaaactga tgcgcgattc cctctcgatg
      601 cagctgttga acatcgactt ccatcgcgtc tctgtcacgg acgttatcac caaccggatt
      661 cccgtagagc tggtgggaga gccgcgcggc gtcagcgagg gtggaacgct gatgcagcca
      721 ctcatcgacg tcgaagtccg cggcgtcgcc gcctcgctgc ccgcgtctct gcaggtagat
      781 gtctcgcacc tgggaatcga cgagagcctg cacgtgagcg aaatctctgt tcctgagggc
      841 gtcgagatcg tggctgcgcc cgacgaggtg gtggcggtgg ttcacgcgcc gcgcgccgct
      901 gtcgaggagc cggtggagcc cgagcagatc accgagccgg agctggtcgt gaagaaggaa
      961 gaggaagagt aatccgcttc cgcgtaaggc agcggggaga tcccgaggga tctccccgct
     1021 gccttttaaa gcgggcgtca gaactgggtt cgtaccgcca ccggtttgcc ggtcgccgcg
     1081 gattccaccg ccgcgcgaca ggccgccacg gtgcgcgccc cctcccgacc atcggtcaat
     1141 agtggcttcc cctccaagat gcactccacc agctcgttca cctctgccgt gacgttgtgc
     1201 gatgccagat tcacgggcaa cgagatccag ccgttgctct ctggaaggtc cttcgtgtaa
     1261 agctggatgg cgggcgaggt gttatcgcag atgatcgtgc cgcgggtgcc atagaacacg
     1321 gaccgcattg tgtagggtcg cttgcatcca atggagcaca tcactttgcc gatcacgcca
     1381 ctttcgaact tgtaaatcgc gatcgtgcag tcgtcgaccg gccaatccgt caacacctta
     1441 tggttgctat acgccgagca ctccaccatg ttcccggcga cccaccggag aagatccacc
     1501 gcgtggcagc caccgccgac gaacggctcg cgcttcactt tgggatcctt gcgccagcca
     1561 ccgaccccgc cgacgtgcgt atagtcgtgc gcgtactccg actccacgaa gaagaggtcg
     1621 ccgatctccc cccgatcgac caggcgtttg gcaagcgcaa atccgggcgc gaaacggcag
     1681 acctgcccca ccatgagcag cttgcccgtc cggtcggcgg cggcgatcat ccgctcgcac
     1741 tccggcacgg tgagcgccat cggcttctcg cacagaacgt gcttgcccgc ttccagcgag
     1801 cgcacggtca tcggctcgtg aagcggatct ggcgttgcca cagagatgat ctggatatca
     1861 tctcgctcca gcagctcttc ccagttatcg gtcaccagcg gcaccgagtg cttctctgcc
     1921 acccgccgcg cgctctccag gtcggtatcg cagacggcgc ggagctcggt gtgcgggttg
     1981 ttgacgtagc ccagcacatg tcccgaaccc atacgcgcgc cgattaccgc agctcccagc
     2041 gtagacatcg acgcaaactc ctttcccggg tccaccccta gcgcgtggac cgtaactccg
     2101 ttcgcgcgca gtgcggcaac tcctctcccc tgctcttcca agctcgcgag gccctgaacg
     2161 atgtttggta tccctactgc cctgggaata ggttggatga cacccctgcg aaggagaggg
     2221 cgcctatctc tccccggcgg catggaacga acggcgaatg ccgccgaaag cgttcagagg
     2281 aggttacgat gatcaagctt cgtgtcgtga tggccgcgct cgcggtcctg gtactggcgg
     2341 cgtgtgcgcc acctgcctcc gctcaggagc aaggccggct ggcaatcacc ggtgggctct
     2401 cctggttcac gggagatgtc aatgacgaca acacgtggat tgccgggctc caatactcgt
     2461 tccccaccat gaccgggcag cggtcaaggg gtaccttcct gctcagtgcg gacttcctcc
     2521 aggtggactc gacccggctg gatgcgggta tcccggtgac ggatcacgtg aatctgatcc
     2581 ccgtgctgct caactacagg cattcgctgg gtgccacgcc gtggtacatc ggcgccggtg
     2641 ccggtatctt cctctcggag gagaacatcc ccgagatggg catcagcagc gatgtgaact
     2701 tcgcctggca gctcttcgcg ggctacaatc tcggcaacaa ctggttcgtc gaggggcggt
     2761 ggctggcagg caacgaccca ggtgacgacg gcatgctgat cgcgcaactg gggttccgct
     2821 tctagcgctt gcagaaggag agccgcagca ggtgggaggc acgttccttc atccgcgggt
     2881 tcatatgcgg ctctccttct gccacaatgc gtgtgggccg gcggaccgcc ggccgtgtct
     2941 agcgccgccg tcaaccacct ggcaagcacg cgccaccaga tgacgccgat cccggatccg
     3001 caggcttgct ggcaaaagta gagaccgccc ggcgggtctt cctgctgccg caccggggac
     3061 acggcgtgcc agtacgcggc gcgctcatcg gctgtagctc agagaagagt gcatggcact
     3121 cctcgcaacg gtactcatag agtggcatga ttgcaactcc cccttcacgt cgcttccggc
     3181 cgggccgaag cggaccgtcc cattggatac gcgcgggcac ttcctggatt cgcatcctgg
     3241 cgcctctctc accctgcggt tgacactgcc ctcccggatg cgctatgcta gcacttgccg
     3301 ggaaagccgg cccgattgca acctcgaatg ccgggctgcc cggactacac cttcaggatt
     3361 gaagaaccaa gatgcgaatc gtcatagccc cggactcgtt taagggaagt ctgagcgcgc
     3421 aagaagtgtg cgacgcgatt gcccgcgggg tgctgcgcgc cgcccccgaa accgtcatcg
     3481 acaccgtgcc gatggcggat ggcggcgaag gcaccgttca gtccctggtg gatgccaccg
     3541 gcggagagct ggtgatgcag cacgtcaccg gccccctggg cgagcccgtc actgccacat
     3601 tcggcctcct cggcgatggg gagaccgctg tgatcgagat ggcgtccgcg tctggcctcc
     3661 ccttggtacc ggaaggccgt cgcaacccac tcgtgaccac gacccgaggc accggcgaac
     3721 tcatgcgtgc cgcgctcgac cgcggtcgca ggaagctgct gatcgggatt ggtggaagcg
     3781 cgaccaatga cggtggcgcg ggtatggcgc aggcgcttgg cgccgcgctc ctcgacggca
     3841 atggccggcc aattggcccg ggcgggggag cgcttgcatc cctggcccga atcgatctct
     3901 cgggcatgga cccacgcctg gccgagaccg aggtggtcgt cgcctgtgac gtggagaatc
     3961 ccctgacggg cgcgcgtggc gcgtcggctg tctacggccc acagaagggc gccaccccgg
     4021 agatggtccg ccagctcgat gcgaacctgc gccactatgc cgagatcatc cggcgcgacc
     4081 tgggcaagga tgtggccgag gtgcccggcg ccggcgcggc gggtggcctg ggcgccggcc
     4141 tgatcgcctt cacgggcgcc agccttcgcc gcggcgtcga gattgtcatc gaggcggtga
     4201 ggctccgcga gcggctgcgg ggcgccgacc tggtgatcac tggagcagga cgcaccgatg
     4261 ggcagacggt ctttggaaag acgcctgcag gggttgcgcg cgttgccggt gaactcggga
     4321 tcccggtggt aggcctggtc ggctcctact cctcggatgc gggggttgtg catgcgcacg
     4381 gtatcgacgc gctggtgagc atcctgcacg agccgatcac tgtggccgag gcgatcgcgc
     4441 agggagccac tctgatcgag cgtgctgccg aggagttgat gcgcctgctt ctagtaggcc
     4501 agcgtctgcg gtaagcccgg ctttcgttcc cgcgggcgct tccattcccg ggttgtgctc
     4561 acacgcgccg gccagatcgg cctgcctgcc aacgatgccg tggcggtgtc gccttctatg
     4621 gcatcgccgc attcgcgcgc ggcgcggggc tcgccgctgc tgtcggacgg tgccccgcgc
     4681 gccggatcag gaggcttctg atgcttgctc ttcggatctc gactgcggta ctcgtcacgc
     4741 tcctggctgc tggcccagcc ctcgccgcgg aggcggcaaa gcggctcccc tggcagcgcg
     4801 aagtagacca gcccgatccc acggcagtca agccgctccc ggatctggtc tccgaacgct
     4861 ggagatcaac gggaggcgcc gaggggagcg tggccccatc ggcagagaac gggccctggg
     4921 gtgcccccta cctccg
//
LOCUS       DAOSCV010000236         4913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10838_length_4913_cov_3.295801, whole genome shotgun sequence.
ACCESSION   DAOSCV010000236 DAOSCV010000000
VERSION     DAOSCV010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_4913_cov_3.295801"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLY56_07175"
     CDS             complement(<1..698)
                     /locus_tag="PLY56_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOJ21298.1"
                     /translation="MLVPLIMAGGKGTRLYPASREARPKQLLRVFSDLTMIQETVARI
                     RDLASPEQIFIGTTLELLDRIREQVPEVPPDNYIIEPIGRDTAPAIAYAALTIQRRYP
                     DAVMAVLSADQVIKPASRFHEVLTTAVQAATETHGLITMGIRPNRPETGYGYIKPGKP
                     QGAVYAVERFTEKPDRETAARFVEEGYLWNSGMFVWEVAAICDALRAHVPDIYEGIRG
                     IVDARRPAEEVFPTL"
     gene            complement(793..1884)
                     /gene="holB"
                     /locus_tag="PLY56_07180"
     CDS             complement(793..1884)
                     /gene="holB"
                     /locus_tag="PLY56_07180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOJ21299.1"
                     /translation="MRMEELVGHRRPTALLQRSLLNGRVSNAYLFVGEEGIGKTLAAI
                     AFGRALVCENPDARPEHGIYEACGHCRSCLLSAAGNHPDLRVIAPEGERIRIDQMRDL
                     RHEVSLTAFGGARRAYIIQHAEAMTEESANCILKTLEEPPPGVTLILVTESLSQLLPT
                     ILSRCQIVRFRPAAPGAIEALLVERHHVPAAHARFIASISGGRAGWAVRAAQQPAILD
                     VRERLMHLLARLPEADPVFVFRAAEEIRDMAVALADAGTPEPSAGEAVSASGASSEDR
                     QIRANADAVLDIARAWFRDLLVLKMASEMPLINVDRRGELETAAERTPLPRIHKALDA
                     LSHTRYYLARNANVSLALEVMMARLVVGG"
     gene            complement(1888..2217)
                     /locus_tag="PLY56_07185"
     CDS             complement(1888..2217)
                     /locus_tag="PLY56_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020620805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ21300.1"
                     /translation="MKLVTAVIHDRDKNRMFDVLVTAGFPFTKIASTGGFLREGNVTL
                     LLGVEDERLEELLSLIQENCKTREQYVNILPPDTRPIGTFLPQPVKVEVGGAIVFVVD
                     VERFERF"
     gene            complement(2397..3017)
                     /gene="tmk"
                     /locus_tag="PLY56_07190"
     CDS             complement(2397..3017)
                     /gene="tmk"
                     /locus_tag="PLY56_07190"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ21301.1"
                     /translation="MARGLFITFEGPEGAGKTTQAHLLGEELRRVGHEVQVTREPGGD
                     PLAESIRALLQNPELALVPRAELLLFLAARAQHVERVIRPALEDGKIVLCDRFADSTV
                     AYQSYGAGLLLESVHQLNHFATSGLEPDLTLLLDLDVSLGLQRQDDWNRMELRGVEFH
                     RRVRAGFLALAKEHPKRIRVLDASRPVPEVARDIWACVEALLRERS"
     gene            complement(3017..4603)
                     /locus_tag="PLY56_07195"
     CDS             complement(3017..4603)
                     /locus_tag="PLY56_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOJ21302.1"
                     /translation="MAIATPVSDAAEQRAVTDNLEQLLEVLPPAIRSRMEAHNHLEDL
                     LEIVLDLGREAEARFPDQVVYLGVTVMQEDIDHVTSRVGVFGKDNRAGIERTLHRISA
                     IRNRAGRVIGLTCRIGRAVYGTIDIIRDVVEEGHSILLLGRPGVGKTTKLREVARVLS
                     DELKKRVVIVDTSNEIAGDGDIPHPAIGHARRMQVSTPDQQHAVMIEAVENHMPQVVV
                     IDEIGTEQEAFAARTIAERGVQLVATAHGNTLDNLLMNPTLSDLVGGIQAVTLSDEEA
                     RRRRTQKTVLERKAPPTFDVLIEIMEMDKLAIHHDVAGVVDRMLRGIPPRPEIRVRNP
                     DGEVEILIGQEPEEAPVPEYEGGNGRTAREMLAPEERPVRRPGATVRIFPYGVSRSRL
                     EKAIRDLRVPAVITKDWHEADVLLTLKAHYRREPGKLKEIVGEEVPVYVIKSNTYVQV
                     ASALRDMFQMRDEGSDEENAQREAEEAAERVLAVDEPVELAPQNAALRRLQHLVAQQY
                     QLHSESVGTEPNRRVRISRA"
BASE COUNT          792 a         1621 c         1588 g          912 t
ORIGIN      
        1 agggtgggga agacctcttc tgcgggccgg cgagcgtcga caatcccgcg aattccctca
       61 tagatatcgg gaacgtgggc ccgcagggca tcgcagatag ccgccacctc ccagacaaac
      121 atcccactgt tccagaggta gccctcctct acgaagcggg ctgccgtctc tcggtccggc
      181 ttctcggtga agcgttctac cgcgtagact gcaccctgcg gcttgccggg cttgatgtag
      241 ccataccctg tctccgggcg atttggccgg atgcccatgg tgatcaagcc gtgcgtttca
      301 gtagcggcct ggacggcggt ggtgaggact tcgtggaagc ggctggccgg tttgatcacc
      361 tggtctgcgc tcaggacggc catcacggcg tctgggtagc ggcgttggat ggtgagcgcg
      421 gcgtaggcaa tcgccggcgc ggtatcgcgg ccgatgggtt caatgatgta gttatccggt
      481 ggtacttccg gtacttgctc gcggatccgg tccaggagtt cgagcgtcgt gccgatgaag
      541 atctgctcgg gagacgccag gtcccggata cgggcaaccg tctcctggat catggtgagg
      601 tcgctgaaga cacgcagcaa ctgctttggg cgtgcttccc tgcttgccgg gtagaggcgt
      661 gttcctttgc caccggccat tatcagtgga accagcattc gttcctccca ttcatgccag
      721 gcgcgaacca attccgggcg gcgggtatga tgcggggaag agaagtgcca tgccctaggg
      781 gcacggcacg gctcacccac cgaccaccag tcgagccatc atcacttcca gggccaacga
      841 gacgttggca ttgcgcgcga ggtagtagcg cgtgtgcgaa agagcgtcca gagccttatg
      901 gatgcggggt agcggggtgc gctcggcggc ggtttccagc tcgccacgcc gatcaacgtt
      961 gatcaggggc atctcgctcg ccattttcag aacaagcaga tcacggaacc aggcgcgcgc
     1021 gatatcgagg acggcatcgg cgttggcgcg aatctgccgg tcttcgctgc tggcgccgga
     1081 agcggagacc gcctctcccg ccgacggctc tggcgtcccc gcgtcggcca gggcaaccgc
     1141 catgtcgcgg atctcctcgg ccgcgcggaa gacgaagacg ggatctgcct cgggaaggcg
     1201 cgcgagcagg tgcatcagcc gctcgcggac atcgagaatg gcgggttgtt gcgcggcgcg
     1261 cacggcccag ccggccctcc cgccggagat gctggcgatg aagcgggcat gagcggccgg
     1321 cacatggtgc ctctccacga gcagcgcctc aatggccccc ggcgctgccg gacgaaaccg
     1381 aacgatctgg caccgcgaga ggatcgtcgg aagaagttgc gacaggctct ctgttaccag
     1441 gatcagggtg acgccgggcg ggggctcttc cagcgtcttc aggatgcagt tggcagactc
     1501 ctcggtcatc gcctctgcgt gctggatgat gtaggccctg cgggcgcctc cgaaggcggt
     1561 cagcgagacc tcatggcgca ggtcgcgcat ctgatcgatc cggatgcgct cgccctcggg
     1621 tgcaatgacg cgcagatcgg gatggttgcc agccgcgctg agaaggcacg accggcaatg
     1681 cccgcacgcc tcgtagatcc catgctccgg gcgcgcgtcg gggttctcgc agacgagggc
     1741 gcgcccaaag gcgatggcgg ccagcgtctt gccgatcccc tcttctccca cgaacaggta
     1801 ggcgttggag acgcgtccgt tcagcaagct cctctgcagc agtgctgtcg ggcggcgatg
     1861 gcctaccagt tcctccatgc gcatctgtca gaacctctcg aaccgctcca catccacgac
     1921 gaagacgatg gcccctccca cctctacctt gaccggctgc ggcaagaagg tgccgatggg
     1981 gcgggtatcg ggcggcagga tattgacgta ctgctcgcgc gtcttgcagt tctcctggat
     2041 gagcgagagc agctcctcaa gccgctcgtc ctcaactccc aggagcagag tgacgttgcc
     2101 ttcacgcagg aagccgcccg tgctcgcaat cttggtgaag gggaagccgg cggtcacgag
     2161 cacatcgaac atccgattct tgtctcggtc gtggatgacc gctgttacga gcttcatggt
     2221 atctccgtcc taaccggaaa tgcggctaaa cgcgcgctcg cgcggagcgc cagcgttttg
     2281 tcgccgtgct gcacgaggag gcgctctgcg ttccgtctcc gtctggtcct atgcgctccg
     2341 cgatccgccg aaccggccgg ctcaccggat gagggcccag gctgggacgc ggctggttac
     2401 gaacgctcgc gaaggagcgc ctcgacgcaa gcccaaatgt cgcgggcgac ttcaggcacc
     2461 ggacggctgg catccagcac gcggatccgc tttgggtgtt ccttcgcgag agccaggaag
     2521 ccggcgcgca cgcggcggtg aaactccacg ccacgcaact ccatgcggtt ccaatcgtcc
     2581 tggcgttgca gacccaggct cacgtccaga tcgagcagga gcgtcaggtc cggctcgaga
     2641 ccactggtcg cgaagtggtt cagctggtga acgctctcca gcagcaagcc ggcgccgtag
     2701 ctctgataag cgaccgtcga gtcggcaaag cggtcgcaga ggacgatttt gccatcctcg
     2761 agcgccgggc ggatgacacg ctcgacgtgc tgcgcgcgcg ccgcgaggaa gaggagcaac
     2821 tctgcccgcg gcacgagtgc cagctcggga ttctgcagca gcgcccggat gctttcggcc
     2881 agggggtcgc cccctggctc gcgggtcacc tgcacctcat gaccgacgcg gcgcagctct
     2941 tcgccgagga ggtgcgcctg cgtcgtcttc ccggccccct cggggccttc gaatgtgatg
     3001 aagaggccgc gtgccactag gccctcgaga tccgcacgcg ccggttcggc tcggtgccaa
     3061 cgctctccga gtggagttga tactgttgtg ccacgagatg ctgaaggcgg cgcagagccg
     3121 cgttctgggg tgccagctcc accggttcgt ccacggcgag aacgcgttct gctgcttcct
     3181 cggcctctcg ctgtgcgttc tcctcgtcgc tgccctcatc ccgcatctgg aacatatcgc
     3241 gcagcgccga agccacctgg acgtaggtgt tgctcttgat cacgtagacc ggcacttcct
     3301 cgcccacaat ctccttgagt ttgccgggct cacggcggta atgcgccttc agcgtcagga
     3361 gcacatccgc ctcgtgccag tctttggtga tcaccgcggg cactctcaga tcgcgaatcg
     3421 ccttctccag gcgactgcgg ctgactccgt acgggaagat gcgaaccgtt gctccgggcc
     3481 ggcgcaccgg ccgctcctcc ggagcgagca tctcccgggc agtgcgtccg ttgccgccct
     3541 cgtactctgg gacgggcgct tcctccggct cctgtccgat caggatctca acttcgccgt
     3601 ctgggttccg aacccggatc tccgggcggg gcggaatgcc gcgcaacatg cggtcgacga
     3661 ccccggccac atcatggtgg atggcgagct tgtccatctc catgatctcg atcaagacgt
     3721 cgaaggtggg cggagccttc cgctccagga ccgtcttctg tgtgcgccgg cggcgtgcct
     3781 cctcatctga gagggtgacc gcctggatgc cccccacgag gtcggagagc gtcgggttca
     3841 tcaacaggtt gtcgagcgtg ttgccgtgcg ccgtggccac cagctggacg ccgcgctcgg
     3901 cgatggtgcg cgccgcgaac gcctcctgct ccgtgccgat ctcgtcgatc accactacct
     3961 ggggcatgtg gttttcgacg gcttcgatca tcacggcgtg ctgctggtcg ggcgtgctca
     4021 cctgcatccg ccgcgcatgc ccgatggcag ggtgggggat atcgccatcg cccgcgatct
     4081 cgttcgaggt gtcaacgatc accacgcgct tcttcaactc gtcggaaagg acgcgcgcca
     4141 cctcgcggag cttggtcgtc ttgcctacgc ccggtcggcc cagcaggagg atggagtggc
     4201 cctcttccac gacgtcgcga atgatatcga tggtgccata caccgcgcgc ccgatccggc
     4261 aggtgaggcc aatcacccgt ccggcgcgat tgcgaatcgc ggagatccgg tgaagcgtcc
     4321 gctcaatccc cgcccggttg tccttgccaa agacgcccac acggctggtg acgtgatcga
     4381 tatcctcctg catcacggtc acgccgagat agacaacttg atctgggaag cgcgcctcgg
     4441 cttcgcggcc cagatccagc acgatctcca gcaggtcctc caggtgattg tgggcctcca
     4501 tccggctccg aatggcgggc ggcagaacct ccaggagttg ctccagatta tctgtcacgg
     4561 cccgttgctc ggccgcgtcc gaaaccggag ttgctatggc catttgtctc cctgctatcg
     4621 tttgggttgc ccttcgcgct cccatgccgt tctcgcgtcg ttggtgcgat tattcctctt
     4681 ggatccgctc tccaaccatc acgccgtcgg accgagtggg tccgcggctc gttgcctccc
     4741 gcgggtggct cctcaccgag cccatcgcca cgcgccgggc atcaagagcg acgccggagt
     4801 gatgttcggg cagaaacgcc ggcccggcgg aaggggcacc gcgacggcac tcccactccc
     4861 ttcccatttc ccccggcccc gttgccagga ggatctggtg cgtactggaa gcc
//
LOCUS       DAOSCV010000237         4893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10882_length_4893_cov_3.223853, whole genome shotgun sequence.
ACCESSION   DAOSCV010000237 DAOSCV010000000
VERSION     DAOSCV010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10882_length_4893_cov_3.223853"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLY56_07200"
     CDS             <1..1224
                     /locus_tag="PLY56_07200"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOJ21303.1"
                     /translation="EAGACDGVLLDLHGAMVTETLEDAEGDLIASVREVVGPTVPIIA
                     TLDLHANVTPLMARHATALIGFDEYPHSDMYKRGVEAALMMGAALRGELRPVLASVQL
                     PLLTMPPRQCTLCEPMRTLLRKAHEMEQEPGIANVTLSLGFPFADIRDAGVCVSVTAD
                     RSAELARRKAAEMARAIWEKRDDFTVKLTPVTEALEYVRSRPNGLVVLADGSDNPGGG
                     APCDGTEILRQLVDADARDAVVAVIADPEAVAQAAAAGVGSTITLEVGGKTDDQHGAP
                     VRLTAYVRLLSDGEFVQKGPMGRGVRACMGRTAVLVAGGVEVVVCERRIQPYDTALLR
                     SVGIEPTDRRLIALKSAVHFRSTYEEIAERIFDADTPGVHRPDFAQYEYRRLRRPIYP
                     LEQVPEGESALSFLR"
     gene            1338..2471
                     /locus_tag="PLY56_07205"
     CDS             1338..2471
                     /locus_tag="PLY56_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21304.1"
                     /translation="MQEPTYRVAIIGTGGIANHHAGYYAKHPRTTIVAAADIRPDALA
                     AFCEKHNVPARYTDYRELLEKEQPDLVSVCTWTATHPEVTIAAAEAGVRGILSEKPMS
                     AHLGDARRMVAVCQSRGIPLAIHHQRRFEPGLVEARKRIAAGQIGVPIAGNFRTVGGF
                     LNNGTHGVDFHRYLLGDPAPEWVMTQLERRTNRYERGVLVEDRILSSIRFENGYTLTI
                     EVDMDEKPAPHWWVFGTEGTIRFGQGGTVLFARGEPEPLAAESGPGPLDELIAWIEGG
                     PESRNAGHIALVAHEIMMASYESARTRSRVRVPLDAGVERSPLYTMHEEGLLPVSGPA
                     YDIRSLDALRYAFEREGIPLTGEEYLEGQGNWTGQGLAGQVRK"
     gene            complement(2563..3060)
                     /locus_tag="PLY56_07210"
     CDS             complement(2563..3060)
                     /locus_tag="PLY56_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21305.1"
                     /translation="MSNRCFRPVRPEDVERIAQIAVRAWQPIYAYYREALGEELFARL
                     HTGWEERKADQVRQAARARPDCVYVTEIDGAIAGFITFMVNPETGVGEIGNNAVDPEC
                     QTSGIGTAQHREALRIMRERGMHTARVTTGLDPAHAPARRSYEKAGFDHSTSSITYYM
                     DLSDL"
     gene            complement(3159..4418)
                     /locus_tag="PLY56_07215"
     CDS             complement(3159..4418)
                     /locus_tag="PLY56_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ21306.1"
                     /translation="MNASYNPASVVVIAAAVVLLLVLLRARSPLLTVALIGPLFFFIV
                     AAILHASQLFVMGAMVLGIALLSYLLSTLSLHHVAVSREVPSPLTAGEAAPFTLTVEN
                     KGRLPKVFLRISAGIPPGIDAVPKHHLLAVLWPGQRASLEGVITAPTRGAYPLAPVEV
                     VATDPLGLFHRRRALPGTGEILVYPRICRIEYHEYLGESAYGSTSPRHSVLPGHGLDF
                     HSLRDYQPGDDLRRVDWKTSARMGRMVVIEFEPVTVGDVLVVLDSRASGPPLELAVTA
                     AASWLGQVLDQGGQGRLRFARGKTVDQVDGYGPEAIPLLLEALARVEPNAKLSPADLA
                     EGVLPGRTTLIITPSADQAVVEAAIRLQSGGETLVLLIDAAQPGRAGRRTAAATQVNA
                     TRLRHHGLRVVVVGAADLQVPAEYPTH"
     gene            complement(4415..>4893)
                     /locus_tag="PLY56_07220"
     CDS             complement(4415..>4893)
                     /locus_tag="PLY56_07220"
                     /inference="COORDINATES: protein motif:HMM:NF036831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ21307.1"
                     /translation="PLPLAQLDRFLMRLSLGYPSNEEELLLLERQMKRHPIEDVKPVV
                     TPEEVVTLQEMVTSIHIERALREYVVKLVDATRRHSAIHFGASPRGSLGLMRASQAYA
                     ATAGRTYVIPDDIKAVAPAVLTHRISLKPEVRARGITAEGTVEEILRTVPVPVLRR"
BASE COUNT          811 a         1610 c         1701 g          771 t
ORIGIN      
        1 gaggcgggcg cgtgcgatgg cgtgctcctc gacctgcatg gcgccatggt gacggagacg
       61 ctggaggatg ccgaaggcga cctcatcgcc tcggtccgcg aggtagtcgg cccgacggta
      121 ccgatcatcg ccacgctcga cctccatgcc aacgtcacgc cgctgatggc ccggcacgcc
      181 accgcgctca tcggcttcga cgagtacccg cactctgaca tgtataagcg cggggtggaa
      241 gccgcgctga tgatgggcgc ggcgctccgc ggcgagttgc gtccggtgct tgccagcgtg
      301 cagctcccgc tcctcacgat gccgccgcgg caatgcaccc tgtgcgagcc gatgcgcacg
      361 ctcctccgca aagcccacga gatggagcag gagcccggga tcgccaatgt caccctctcc
      421 ctcggctttc ccttcgccga tatccgcgac gccggggttt gcgtgtcggt gacggcggat
      481 aggagcgccg aactcgcacg gcgcaaggct gcggagatgg cccgagcgat ctgggagaag
      541 cgggacgact tcacggtgaa gctcactccg gtcactgagg cactggagta tgtgcgctct
      601 cgcccaaacg gcctggtcgt cctggccgat ggctcggaca accccggcgg tggcgcgccg
      661 tgtgatggca ccgagattct gcgccagctg gtcgatgcgg atgcccgaga cgcggtggtg
      721 gccgtcattg ccgatccaga ggcggtggca caggcagccg cagccggcgt ggggagcaca
      781 atcaccctgg aagtcggggg caagaccgat gaccagcatg gcgcgccggt gcgcctgaca
      841 gcctatgtgc gcctgctctc ggacggcgag ttcgtgcaga agggaccgat gggcaggggg
      901 gtgcgtgcgt gcatgggccg aacggccgtg ctggtggctg gcggcgtcga ggtggtcgtc
      961 tgcgagcgcc ggattcagcc ctacgatacg gcgttgctcc gcagcgtggg gatcgagccg
     1021 acggaccgcc ggctgatcgc gctgaaatcc gcggtgcatt tccggagcac ctatgaggag
     1081 attgccgagc ggatcttcga tgccgacacg cccggcgtgc accggcccga ctttgcccag
     1141 tacgagtacc gccggctccg ccggcccatc tatccgctgg agcaggttcc agagggagag
     1201 tccgccctct ctttcctgcg ctgagggcag atgtgctgag ccttgggaat tgataacgcg
     1261 gtgggggcag aaacgagcgc ccgctgagtc tggtacctga cgagcgactg aactgcctcc
     1321 tggagagaga agaagcgatg caggagccga cctatcgcgt cgccatcatt ggcacggggg
     1381 ggatcgccaa ccaccacgcg gggtactacg cgaagcaccc gcgcacgacc atcgtggcgg
     1441 ctgcggacat ccgaccggac gcactggccg ctttttgcga gaagcataac gtgccggcgc
     1501 gctacaccga ttaccgggag ctactggaga aagagcagcc cgacctggtg agcgtttgca
     1561 cctggacggc gacccatccc gaggtgacga tcgccgccgc ggaggcaggc gtgcgcggca
     1621 ttctctcgga aaagccgatg agtgcgcacc tgggggatgc ccggcgcatg gtggccgtct
     1681 gtcagtcgcg gggcattcct ctcgccattc accaccagcg ccgtttcgag cccggactcg
     1741 tcgaggcgcg caagcggatc gctgccgggc agatcggcgt gccgattgct ggcaatttcc
     1801 gcactgtcgg cggcttcctc aataacggca cgcatggcgt cgatttccac cgctacctgc
     1861 tcggcgatcc ggctcccgaa tgggtgatga cgcaattgga gcggcgcacg aaccgctacg
     1921 agcgcggagt tctggtagaa gaccgcatcc tctccagcat ccgcttcgag aatggctaca
     1981 ccctcacaat tgaagtggat atggacgaga agcccgcgcc ccattggtgg gtcttcggaa
     2041 ccgagggcac gatccgcttt ggccaggggg gcacggtcct cttcgcgcgc ggcgagccgg
     2101 agccgcttgc cgcggagtcc ggccctggcc cgctcgacga gctgatcgcg tggattgaag
     2161 gcgggccgga gtcgcgcaac gccgggcaca ttgccctcgt cgcccatgag atcatgatgg
     2221 cctcctacga gtcggcgcgc acgcgctctc gagttcgcgt gccgctcgac gccggagtgg
     2281 agcgctcgcc cctctacacc atgcatgaag aaggcctcct gccggtctcc ggcccggcct
     2341 acgatatccg gtcgctggat gcgctccgct atgccttcga gcgcgagggg attccgctca
     2401 cgggcgagga gtacctggag ggtcagggca actggaccgg ccagggtctt gctggccagg
     2461 tgcgcaagta acgccaccga ccggggcagg aaaggcaggc gaagccggcc ggccaggaac
     2521 cggcgccacg ggcgcgcgcc cgtggcgccg gctctgccgc gttcagaggt cgctcagatc
     2581 catgtagtag gtgatgctac tcgtggagtg gtcgaagccc gctttctcgt aagagcgccg
     2641 ggcgggcgcg tgcgccggat cgaggcccgt ggtcacgcgg gcggtgtgca tcccgcgctc
     2701 gcgcatgatc cgcagggctt cccgatgctg cgccgtgccg atcccggagg tctggcactc
     2761 tggatcaacc gcgttgttgc cgatctcgcc gacacccgtc tcgggattga ccatgaaggt
     2821 gatgaagcca gcgatggcgc cgtcgatttc ggtgacgtag acgcagtcgg gccgggcgcg
     2881 cgcggcctgg cgcacctgat ccgccttccg ttcctcccag ccggtatgca gccgggcgaa
     2941 aagctcctcg cctagcgcct cgcgatagta ggcatagatc ggctgccatg cccggacagc
     3001 aatctgcgcg atccgctcca catcctccgg gcgcacgggt cggaaacacc ggttgctcat
     3061 gcttctctcc gtttcggttg ggacggcgcc cgccgtccca gatgcatctc tcactggcag
     3121 gtgacttcgg cggggaatgg cgcgttccct cccgcggcct agtgcgtggg atactccgcg
     3181 ggaacctgca ggtcggcggc gccaacgacg accacccgga ggccgtggtg gcgcaatcgg
     3241 gtagcattca cctgcgtggc ggccgcggtc cggcgcccgg cgcggccagg ctgcgcggca
     3301 tcgatcagga gcaccagtgt ctcgccaccg ctttgcagac ggatggcggc ttccaccacc
     3361 gcctgatcgg cggatggcgt gatgatgagc gtggtccgcc ccggcaggac gccttcggcc
     3421 agatcggcgg gcgagagttt ggcattcggt tccacccgcg ccagcgcctc cagaaggagg
     3481 gggatcgcct ccggcccgta gccatccacc tgatccacgg tttttccgcg cgcaaaacgg
     3541 agtcgcccct ggcccccttg atccagaacc tggcccagcc acgacgccgc ggcggtgacc
     3601 gccagctcca gtggcgggcc agaggcgcgc gagtcgagga ccacgagcac gtcgcccacg
     3661 gtgaccggct cgaactcgat gacgaccatg cgtcccatgc gcgcgctggt cttccaatcg
     3721 acgcggcgga gatcatcccc cggctggtaa tcccggaggc tgtggaagtc gagcccgtgg
     3781 ccgggcagca cggagtggcg cggcgacgtg ctgccgtagg cgctctcacc cagatactcg
     3841 tgatactcga tccggcagat gcgagggtac accaagatct cgccggttcc gggcagggcg
     3901 cgccgtcggt ggaagagccc cagcggatcg gtagccacca cctcgaccgg cgcgagcgga
     3961 tacgcccccc gcgtaggcgc ggtgatcacg ccctccagag aggcacgttg gcccggccag
     4021 agcacggcca gcagatggtg tttcggaacg gcgtcaatcc cggggggaat ccccgcgctg
     4081 attctcagaa acaccttggg cagccggcct ttgttctcca cggtcagcgt gaaaggagcc
     4141 gcctcgccgg ccgtaagcgg agagggaacc tcgcgcgaga cggcgacgtg atgaagcgag
     4201 agcgtgctga gaaggtagga gaggagagcg atgccaagga ccatcgctcc catgacgaag
     4261 agctggctgg catggaggat ggcggcgacg atgaaaaaga agagcggccc gatgagggcc
     4321 accgtcagca gcgggctgcg cgcgcgcagg agcacgagga gcagcactac cgccgcggcg
     4381 atcaccacca cgctggccgg gttgtacgag gcgttcatct gcgtagcacc ggcacgggca
     4441 ccgtgcgcag gatctcctcg acggtgccct ccgcggtgat tccgcgcgct cgaacctccg
     4501 gcttgagcga gatccggtgg gtgagcaccg cgggcgccac tgccttgatg tcgtccggga
     4561 tcacgtaggt ccggcccgcg gtcgcggcgt aggcctgcga ggcgcgcatc agcccgagcg
     4621 agccgcgcgg gctggccccg aagtggatcg cggagtggcg gcgagtggca tccacgagct
     4681 tcaccacgta ctcgcgcagg gcgcgctcga tgtgaatgct ggtgaccatc tcctggagcg
     4741 tcaccacctc ctccggcgtg accaccggtt tcacatcttc gatcggatgg cgcttcatct
     4801 gccgctccag cagcagaagc tcctcttcgt tgctcgggta gcccagcgag agacgcatca
     4861 ggaagcggtc gagctgcgcg agcggcagcg gga
//
LOCUS       DAOSCV010000238         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10890_length_4888_cov_3.463066, whole genome shotgun sequence.
ACCESSION   DAOSCV010000238 DAOSCV010000000
VERSION     DAOSCV010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10890_length_4888_cov_3.463066"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLY56_07225"
     CDS             complement(<1..859)
                     /locus_tag="PLY56_07225"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOJ21308.1"
                     /translation="MTPARQERESTAAMRPRRRIVVRGTVQGVGFRPFVYRLACSLNL
                     TGWVRNGPDGVAIEVEGRPDDVARFVPRLQAELPPLARLTHLEEIPLAPEGSTEFSIR
                     DSEEGVRPTALIPADLTTCEQCAAEVADSTNRRYRYPFTNCTDCGPRFTIVEGVPYDR
                     ARTTMRAFTMCPECRAEYEDPRSRRFHAEPNACPACGPRLWLARARERSAVKPDASCP
                     DPIAEAARLLHAGKVVAVKGLGGFHLACDARRGDVVTLLRARKHRDHKPFAVMVSGLA
                     AARALADIDA"
     gene            1066..2631
                     /locus_tag="PLY56_07230"
     CDS             1066..2631
                     /locus_tag="PLY56_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ21309.1"
                     /translation="MVRGRVRQTSHCLEGREPMGNRLRCPAWRWMILVIITLVSALPA
                     AAQPLEERVVEHTLKNGMKFLFVERRVAPIFTGEIVFRAGGVDEQVGATGLAHMLEHM
                     AFKGTRAIGTRNYRKEKPLLDAMDRVAKDLARERARGSAADEKRIEALEREMKRLQEQ
                     ASEYSDGEEFSAIYTRNGGAGLNAGTSKDITAYHVSLPSNRLELWALMESQRMAEPVL
                     REFYVERDVVAEERRRTYDSSPDRKLYEQLVATAYTAHPYGVPIIGWMSDIQSLTADQ
                     ARAFHKTYYVPGNAVAALVGDIDIPQAIRVVEKYFGGIPAGPPPPPVVTVEPPQAGER
                     RVNVVFDAEPQVMIAYHKPTAPHPDDTAFDVLHGLLAGGRTSRLYTSLVKEKRIAAGV
                     GGLGGPGSRYANLWGVQAYPLAPHTTGEVEQAIYAEFERLMREPVPERELQKVKNQLD
                     ADFVRGLSSNRGLASQLTYYEAVVGDWRYLTRWREMIAQITPEDLQRVARKYLVPENR
                     TVATLVRKESAGQ"
     gene            2628..4061
                     /locus_tag="PLY56_07235"
     CDS             2628..4061
                     /locus_tag="PLY56_07235"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ21310.1"
                     /translation="MTRYALLAILSLMLPMPAPARADYPFSRTHPSTLKYPELRFTPP
                     EPVERTLPNGMTLYLMEDRELPLLRVEAYVKTGSIYDPAGKVGTARLTATVMRTGGSE
                     RLQGDAIDEKLEFVGGKLEVSVGDEFTTVSGSALSKDADALLEVLAEVLRRPVFPEEK
                     LALAKAQMMEGIRRQNDQPASIANRVFAQALYGKESPWARQPTIAEVEKLSRADLVGF
                     HQRTYAPNNTVLAIAADMSVDEMARRVERWLGDWERRTLRLPEPEPVKDAARPEVIIV
                     AKETQQANLRVGHLGISRDNADRYPVRVMNHLFGLGGFSSRLMRDVRSSKGLAYAVWG
                     YLGEGRDRGVFETGAETKVASARAAVDAMRQTLSDLLEKPVAPLELSDAQSALVNSFV
                     ARFTSPFQIASQRALLDVLGFPEDYLRSYTREIGAVTAADVHRVARAHLHPDRLVIVA
                     VGPAKELRESMAGLGPVRVISPDETVR"
     gene            4343..>4888
                     /gene="dnaN"
                     /locus_tag="PLY56_07240"
     CDS             4343..>4888
                     /gene="dnaN"
                     /locus_tag="PLY56_07240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ21311.1"
                     /translation="MKVFCRRKDLYEGVQTVGRVVSARSTLPILSHILLQASEDRLRL
                     VATDFEIGMDVTVPASVDVEGAVTVPARLLIDILGSLPDTDVALSVDENNVIELKCLR
                     SEYVLRGLPAQEFPKLPEVEDGRSVEVEQALLRQLINETIFAVSTDETRALLTGALAV
                     LGDGFIKMVATDTHRLAVSTMP"
BASE COUNT          826 a         1573 c         1702 g          787 t
ORIGIN      
        1 cggcgtcgat gtcggccagc gcccgcgccg cggccaggcc gctcaccatg accgcgaacg
       61 gcttgtggtc gcggtgcttg cgcgcgcgca acagcgtgac gacgtcgccg cgccgcgcgt
      121 cgcacgcgag gtggaacccg cccaggccct tgaccgcgac caccttgccg gcgtggagca
      181 ggcgcgcggc ctcggcgatc ggatccggac aagaggcgtc tggctttaca gccgaacgtt
      241 ccctggcgcg cgccagccag aggcggggcc cgcaggccgg acacgcgttc ggctcggcat
      301 ggaagcgccg gctccgcgga tcttcatact cggcccggca ttcagggcac atcgtgaacg
      361 cgcgcatcgt ggtgcgtgcc cgatcatagg gcacgccttc gacgatggtg aaccgcggcc
      421 cgcaatcggt gcagttggtg aaaggatagc gatagcgccg gttagtcgag tcggccactt
      481 ccgcggcgca ctgctcgcac gtggtgaggt cggcggggat gagcgcggtg ggccggacgc
      541 cctcttcgct gtcgcggatc gaaaactccg tgctcccttc cggggcgagc gggatctcct
      601 cgagatgggt gagccgggcg aggggaggga gctcagcctg gagccgcggc acgaagcgcg
      661 ccacgtcgtc cgggcggcct tccacttcaa tcgcgacgcc gtccggcccg ttgcgcaccc
      721 agccggtgag gttgagcgag catgccaggc gatagacgaa gggccgaaac ccgacgccct
      781 gcacggtgcc ccgaaccacg atccgccgcc ggggacgcat cgccgccgtc gattccctct
      841 cttgcctggc tggtgtcatg ctctccctgg tgttaacgaa gaagtgactg ccggcttcat
      901 tataggcgag gaccctggca gcgtcaagca acagacaggc gctcgcggct attgcccgcg
      961 ggccctaaca gagcgagcac ctggcgggat gcgttgccgt ctggtccatg agaggggatc
     1021 cccgacgcag gcgcgcgtgt cttggaatcg aagaggattc ccgcgatggt tcgtggtaga
     1081 gtgcggcaga cgagccactg cctggaaggg agagagccga tggggaatcg actgcggtgc
     1141 ccggcatggc gctggatgat cctggtgatc atcaccctgg tatccgcgct gccggccgcc
     1201 gcccaaccgc tcgaggagag agtggtcgag cacaccctga aaaatgggat gaagtttctc
     1261 ttcgtggaac ggcgcgtcgc cccgatcttt actggggaga tcgtctttcg cgcgggtggc
     1321 gtggatgagc aggtgggcgc tactggcctg gcacacatgc tggagcacat ggcgttcaag
     1381 ggaacgcgcg ccatcggcac gcgcaactac cgcaaggaga agccactcct ggatgcgatg
     1441 gaccgcgtcg cgaaggacct ggcacgcgag cgcgcgcgcg gcagcgcggc cgacgagaag
     1501 cggatcgagg cgctcgagcg tgagatgaag cgtctccagg agcaggcgag cgagtatagc
     1561 gacggcgagg agttctcggc aatctacacg cgcaatggcg gcgccggtct caatgccgga
     1621 acgagcaagg atatcacggc ttaccacgtc agcctgccct cgaaccggct ggagctgtgg
     1681 gcgctgatgg agtcgcagcg catggcggag ccggtactgc gcgagttcta tgtcgagcgc
     1741 gacgtcgtcg ccgaggagcg ccggcgcacc tatgacagca gccccgaccg caagctgtac
     1801 gagcaactcg tcgccaccgc ctacaccgcg catccatacg gggtgccgat catcggctgg
     1861 atgtccgata tccaatcgct gaccgccgac caggcgcgcg cgtttcacaa gacgtattac
     1921 gtgccgggca acgctgtcgc cgctctcgtc ggcgatatcg atatcccgca ggcgatccgg
     1981 gtcgtggaga agtacttcgg gggaatcccg gcggggccgc cgcctccgcc cgtggtgacc
     2041 gtagagccgc cccaggctgg cgagcggcgc gtgaacgtcg tcttcgacgc ggagccccag
     2101 gtgatgatcg cctaccacaa gcccacggcg ccgcacccgg atgacaccgc ttttgacgtg
     2161 ctccatggtc tccttgccgg cgggcgcacc tcgcgcctct acacatcgct ggtgaaggag
     2221 aagcgcattg cggcgggcgt ggggggattg ggcgggccgg gcagccgtta cgccaacctc
     2281 tggggcgtgc aggcctatcc gctcgcccca cacaccaccg gcgaggtgga gcaggcgatt
     2341 tacgcggagt tcgagcgcct gatgagagag ccggttccgg agcgcgagct gcagaaggtg
     2401 aagaaccagc tcgatgcgga tttcgtccgc ggcctgtcgt cgaacagggg tctggcctcg
     2461 cagctcacct actacgaggc cgtggtgggc gactggcgct acctgacacg ctggcgcgag
     2521 atgatcgccc agatcacgcc ggaggatctc cagcgggtgg caaggaaata cctcgttccg
     2581 gagaaccgca ccgtggccac actcgtgagg aaggagagcg cggggcaatg acacgttacg
     2641 cgctgctggc gatactctcg ctgatgcttc ctatgcccgc gcccgcaagg gcggactacc
     2701 ccttctcgcg cacgcatccg agcacgttga agtacccgga gctgcgtttc actccaccgg
     2761 agccggtcga gcgcaccctc cccaacggga tgacgctcta tctgatggag gatcgggaac
     2821 tcccgcttct ccgggtggaa gcctacgtga agaccggttc catttacgac ccggccggca
     2881 aggtgggaac ggcgcgcctc accgctacgg tgatgcgcac cggtggcagc gagcggcttc
     2941 agggcgacgc catcgatgag aagttggagt tcgtgggtgg caagttggag gtctcggtgg
     3001 gcgacgagtt taccaccgtc tccggatccg cgctgagcaa ggacgcggat gcgctcctgg
     3061 aagtgctcgc cgaggtgctg cgccggcccg tcttcccgga ggagaagctt gccctcgcca
     3121 aggcccagat gatggagggg atccgccgac aaaacgacca gccagccagc atcgccaacc
     3181 gtgttttcgc gcaggcgctc tacggcaagg agagcccatg ggctcgccag ccgaccatcg
     3241 ccgaggtgga gaagctgtcg cgcgcggatc tcgttggctt ccaccagcgc acctacgcgc
     3301 ccaacaacac ggtcctcgcc atcgccgccg acatgagcgt ggatgagatg gcgcgccgcg
     3361 tcgagcgctg gttgggcgat tgggagcgcc gcacgctcag actcccggag ccggagccgg
     3421 tgaaggatgc ggcgcggccc gaggtgatca tcgtggccaa ggagacccag caggcgaacc
     3481 ttcgcgtcgg gcacctgggc atctccaggg acaacgcgga tcgctacccg gtgcgcgtga
     3541 tgaaccacct gttcgggctc ggtgggttct cctcgcgttt gatgcgcgac gtgcgctcca
     3601 gcaagggcct ggcctacgcc gtttggggct acctgggcga ggggcgcgac cgcggggtct
     3661 tcgagacggg cgcggagacg aaggtcgcct cggcccgggc cgcggtcgat gccatgcgcc
     3721 agacgctgag tgacctcctt gagaaacccg ttgccccctt ggagctgagc gacgcgcaga
     3781 gcgcgctggt caactccttc gtggcgcgct tcacctcgcc attccagatt gccagccagc
     3841 gggcgctcct cgacgtgctc ggctttcccg aggactacct gcgctcctac acgcgcgaga
     3901 ttggcgccgt gaccgccgcc gatgtgcacc gcgttgcgag agcccacctg cacccggacc
     3961 ggctggtgat cgtggccgtc ggcccggcga aggagctgcg cgaatccatg gccggcctgg
     4021 ggcccgttcg cgtcatctcg ccggacgaaa ccgtccgcta agccggcatc ggcccgccgc
     4081 ggaggcagcc ggtggcattg tctccgcggc ggagggcggc cggtccgtga caaggtccac
     4141 aaaatgccac ccggggagac cgcctccggt aggaggaggg catgcgcccg gcgaactcca
     4201 cccatatggg tttcacaaat cccctcgcag ctccagccga accaccgtca cgtcgcgaag
     4261 ctcaccgcgg gtgccggacg cggcttgact gcgcggccgc gcggtggctg cggccgcctc
     4321 acggattgga aggagcagaa ctgtgaaggt tttctgcagg cgtaaagacc tctatgaggg
     4381 ggtgcagacg gtcggtcggg ttgtatccgc aagaagcacg ctgccgatcc tcagccacat
     4441 actgttgcag gcgtccgagg atcgcctgcg tctggtcgcg accgattttg agattggcat
     4501 ggatgtcacc gtgccggcct cggtggatgt cgagggcgcg gtgacggttc cggcgcgtct
     4561 cctgattgat atccttggct cgcttcccga tacggacgtt gctctctcgg tcgatgagaa
     4621 taacgtgatc gagttgaagt gccttcgatc cgagtatgtg ctgcgcgggt tgccggcgca
     4681 ggagttcccg aagctgcccg aggtcgagga tgggcgctcc gtggaggtgg agcaggccct
     4741 gctgcgccag ttgatcaacg agacgatctt cgccgtctcc accgatgaga cgcgggcgct
     4801 gctgacgggc gcgctggcag tgctcggcga tggctttatc aagatggtgg cgacggacac
     4861 gcaccgcctg gcggtctcca cgatgccg
//
LOCUS       DAOSCV010000239         4875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10919_length_4875_cov_3.616805, whole genome shotgun sequence.
ACCESSION   DAOSCV010000239 DAOSCV010000000
VERSION     DAOSCV010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10919_length_4875_cov_3.616805"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..347
                     /gene="csx15"
                     /locus_tag="PLY56_07245"
     CDS             9..347
                     /gene="csx15"
                     /locus_tag="PLY56_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx15"
                     /protein_id="HOJ21312.1"
                     /translation="MWRCVSELERVIAVPTQFSHDQPFAEQARALIDQIELTPTEWQI
                     APLLINLPSLNTIAAVVIAELHGRMGYFPAILRLRPVAGSVPPQFEVAEIINLQGIRD
                     AARAGRHPGV"
     gene            596..1312
                     /locus_tag="PLY56_07250"
     CDS             596..1312
                     /locus_tag="PLY56_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21313.1"
                     /translation="MWGWIKDLSDFADPINNLVGLLGVLAIIYARLVDAPRRARALLR
                     RLMHGPARPPVLTRKRPEKRRGLILMVSRLEPCEKAIAYHRPVLERCWLICSLQSLEV
                     ARTLMKRHPDVCESAPIVINDVHDPLEYRNAADRIYTQELPEGWSEADVIADFLGMTA
                     QGSVGMTLACLSPSRPLQYTVPHFDNDGKPLEPLDPIEIVLDWSSVVHAEGVGLASGE
                     PSPGQDAGLADAPKQVAKAG"
     gene            1812..2636
                     /locus_tag="PLY56_07255"
     CDS             1812..2636
                     /locus_tag="PLY56_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOJ21314.1"
                     /translation="MAIHIGTSGFSYPDWRGFFYPENLPERDMLAHYSQQFNTVEANF
                     TYYRLPGPATFARMQQMVPPGFRFAVKANRQMTHEGGDGAPAMGAWHRAGPETDAFFA
                     RFVEALAPLAEAAQLACVLVQFPWSFRCCDETREYARRLPERMQGLPTVVEFRNREWL
                     DDSLFELLRECGLGFCCVDEPRLKGLMPPLAVSTSSVGYVRFHGRNARAWWRHDEPWQ
                     RYDYLYTQEELAEWVPRIARLAAQTSDTFVFFNNHYQGKAGKNARMLAQLLLPLPK"
     gene            3378..3731
                     /locus_tag="PLY56_07260"
     CDS             3378..3731
                     /locus_tag="PLY56_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ21315.1"
                     /translation="MDLEIVVRKETGEIAEVALVGRVDMQTAELLKNRLAAVVEEGCR
                     HILLNMRRVKFIDSVGLGSLIGARRRVVEAGGSLRLVHLSPAVSRALEIANLMRLFDV
                     HESTQDAVAALARGA"
     gene            4032..>4875
                     /locus_tag="PLY56_07265"
     CDS             4032..>4875
                     /locus_tag="PLY56_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21316.1"
                     /translation="MPGRLLTECPIWSPDGRALLLTTGGRRIYLLDRTEGRVRFLTYG
                     CDPAWSPDGRQIAFVCLGRRHEVGIIRADGQGGVRILRIPSGVRPPLLWDRVGASLCF
                     ADARGRVIGVRVPDGTPMSWSLPSPVDALAASPDGEWLAFTAPCEMPGVLCLYLYHRR
                     DRSLRVIAQVPPCRLYWTRKGRALTALAEDAAEPSLQYEMATGRVRRLHVPEGLVPLV
                     PSPTDDAMLYLETRAPGQMMVGEAEGNRLRLFAHGYFPAWSPDGESIAFYDNPDEEAS
                     LCMQS"
BASE COUNT          796 a         1566 c         1615 g          898 t
ORIGIN      
        1 tgccgcgttt gtggagatgc gtttcagagc tcgagcgcgt gatcgcggtg cccacccagt
       61 tctctcatga ccaacccttc gcggagcagg cgcgtgcgct tatcgatcaa atagagctga
      121 cgcccaccga gtggcagatt gccccgctct tgatcaacct acccagccta aacaccatcg
      181 ccgccgtggt catcgccgag ctccacggga ggatgggcta cttccctgcg atcctccgct
      241 tgcgcccggt tgctgggagt gtgccccccc agttcgaggt ggccgagatc atcaacctcc
      301 agggcatccg ggatgcggcg cgggccggac gccacccagg cgtgtaggta cggcgcgaga
      361 ggaggataag acacttccga atccggcaac acctggtgcc cagcggtgct acccaggcat
      421 aagcacccgc gcaccggctc gcacatcatc cgatgtggtt gagataatgg atgcagcagc
      481 atcccggggc tgggttccga taagcaccac ccttcggtgc gatgagtgcc actacttcac
      541 cgtggccttt cgggcatcgc tgcttcctgc gtggtatcat taaggaggaa cacccttgtg
      601 gggatggata aaggacctca gtgatttcgc ggaccccatc aacaacctcg taggcctctt
      661 gggagttctc gcaattatat atgctaggct ggtagacgcc ccgaggcgag cccgggcgct
      721 tctgcgccgc ctgatgcacg gaccggcacg ccctccggtg ctgacgcgga agcggcccga
      781 gaagcgccgg ggactgatcc tgatggtgag caggctagaa ccctgtgaga aggcgattgc
      841 ctaccaccgc ccggtgctcg aacggtgctg gctcatctgc tcgctacaga gcctggaggt
      901 ggcgaggaca ctcatgaagc gccaccctga cgtttgcgag agcgccccga tcgtgatcaa
      961 cgacgtccac gacccgcttg agtaccgcaa tgccgcggat cgcatctaca cgcaggaact
     1021 gccagaaggc tggagcgagg cggacgtgat cgccgacttt ctgggcatga ccgcgcaggg
     1081 gtctgtcggc atgaccctcg cgtgcctgtc acccagccgg ccgctccaat acacggtgcc
     1141 ccattttgat aacgacggga agcctcttga accactcgac ccaattgaga ttgtgctgga
     1201 ctggagcagc gtggtgcacg cggagggtgt tggcctggcg tcgggcgagc catccccggg
     1261 ccaggatgcc gggcttgcgg atgcccccaa gcaggttgcg aaggccggct gacccgcctg
     1321 aggcctcttg cgcgtgggag cgatctcttg ctgtccgcca tcgtactcca gttggagccg
     1381 gtcaccgagg cgcgcctgcc gctttcccac ggcgcgttcg ctcatgccgc ggcgatggag
     1441 ctcttcctgc gcctcgaccc ggcgcctggg gtcagattcc ctttgcatcc acaccacggt
     1501 gcctcctgag attcagagat aaggaatccg aagcctcgga ggcattgcgc aacccggcgc
     1561 gcggcgctaa aagcaccgcg ctgctttccg gcaaagccct ttcagggctt ctcctcccag
     1621 ccaccgccgt tttgcaacgt ctgggggtat ccccaaccca gcccggcagg gcttcgctca
     1681 caagcagcgc ggagcttcga gctccgcgcg tgcgcggcac tctccttatc tcggaatccc
     1741 agcggtgcct cgcagactct tgacggcggt ggcgaggggt gttatactgg gatagccggc
     1801 agggagagct catggcgatt cacatcggca cctctgggtt cagctatcca gactggcgcg
     1861 gcttcttcta tccggagaac ctgcctgagc gggacatgct cgcccattac tcccagcaat
     1921 tcaataccgt ggaggccaac ttcacgtact accggctgcc tgggcccgcg accttcgccc
     1981 gcatgcagca gatggttccg cccggattcc gcttcgcggt gaaggcaaac cgccagatga
     2041 cgcacgaggg gggcgatggc gcgccggcga tgggcgcctg gcaccgggct ggcccggaaa
     2101 ctgacgcctt ctttgcccgc ttcgtcgagg ctctggcccc gctggcggaa gcagcccaac
     2161 tggcgtgcgt gctggtccag ttcccatggt ccttccggtg ttgcgacgag acccgggagt
     2221 acgctcggcg tctgcccgaa cggatgcaag gcttgcccac cgtggtggag tttcgcaacc
     2281 gcgagtggct ggatgacagc ctcttcgaat tgttgcgaga gtgcgggctg ggcttctgct
     2341 gcgtcgatga gccccggctg aaggggttga tgcccccgtt ggcggtttcc acgtcgagcg
     2401 tgggctatgt gcgcttccac gggcgcaacg cccgcgcgtg gtggcggcac gatgagccgt
     2461 ggcaacgcta cgactacctt tatacccagg aagagctcgc ggagtgggtg ccgcgcatcg
     2521 cccggctggc agcgcaaaca agcgacacct ttgtcttctt caacaaccac taccagggca
     2581 aggcggggaa gaacgcccga atgctcgccc agctcctcct tccactgccg aagtgacgcc
     2641 ctcccatgac gcgggcgggt tgtgacctcc tcgcgagcga ccccgagcat ataggaggcg
     2701 gcttcatgtt ggcgagccgg gtttccgctg ggttgcatgc cccgacaagc ctggtttgat
     2761 cgcccgcgaa aatggaaagg atggttgcac gggtagtacg cagccagccg atccgagacg
     2821 gtgatgcccc gactcccgcg ggccccatat gcggcaggac aggcgcgtga ggccctggtg
     2881 caggcatcag cggatacgcc gtgcaaggtg gttggagcgg cgggtggcct ggaagcacgg
     2941 gggcctcatc ctgcacagtc gcgcttggcc tctgtctccc ggctctctga tgatcagaag
     3001 ggctccgggg tggtgggacg gctcgccgtt catttctgag cccgttgagc gcgcgtgatg
     3061 gcgctggtga gtgagggttg tggcccgcgc gcgggaaaag cggggcggca ccctacttgc
     3121 agaagcttcc accggtgccg gagcttggca tcgccgacag tgttctcttg ctcgcccgag
     3181 ggcacagaag ggcgttcttc gcaaaatggc ggcggaggtc gcagggccga ttcggccgcc
     3241 gcaggcagga gagcctgccg cgctatcacc cggagtgcgc agagacgcct ccggcattcg
     3301 cgagttttgc tcgtcacccg ccggcccggc caagggccgc cgccagggtg gaattccgta
     3361 ccagagaagg tggaacgatg gatctggaga tcgtagttag aaaggaaacc ggcgagatcg
     3421 ccgaggtggc cctcgtaggg cgggtggata tgcagacggc ggagctcctc aagaaccggc
     3481 tcgctgcggt ggtcgaggaa ggctgtcggc atattctgtt gaacatgcgc cgcgtgaagt
     3541 tcatcgacag cgtggggttg ggttccctta tcggagcccg acgccgagtg gtcgaggcgg
     3601 ggggcagctt gcgcctggta cacctcagcc cggccgtctc gagggccctg gagatagcga
     3661 acctcatgcg cctcttcgat gtccacgaga gcacgcagga cgcggtggcc gcgctcgctc
     3721 ggggagcatg aggcctcctc acgtttagtg gctccaccag cgcctggtaa gccttcgcgg
     3781 cggcaccgtc ctccttgcgt ttctgcgtac ttcccgcgat ctgctttggt gctcgccgct
     3841 gtgggccatg cccttgccgc gtcttctcgc aatcgagcgt tggcttcacg gcgctccccg
     3901 agacgctgcc acttgcccag taggcgcctg tgcaacgcag gaacgccctg gtggctccgc
     3961 gaacactcca ccagatggtg cccgaagctg ccgatttgag gcagcacgcg gagcgcgtga
     4021 ggtggcatgc gttgccggga cgactcctga cagaatgccc catctggtcg ccggatggga
     4081 gagctcttct ccttaccacc gggggacggc gcatctatct cctcgatcgg accgaggggc
     4141 gcgtgcgctt cctgacatat gggtgtgatc ccgcgtggtc gcccgatgga cgccagattg
     4201 cctttgtgtg tctgggccgg aggcacgaag tgggcatcat acgcgcggat ggccagggtg
     4261 gagtgcgcat cctccgaatc cccagcggcg tgcgcccgcc gcttctgtgg gatcgcgtgg
     4321 gtgcctcgct ctgctttgcg gatgcgcggg gccgcgtcat tggggtgcgc gtgccggatg
     4381 gcacgccgat gtcatggtcg ctcccttcgc cggtcgacgc cctcgccgct tccccggatg
     4441 gcgagtggct ggcgttcacg gcgccgtgcg agatgcccgg cgtgctttgc ctctatctct
     4501 atcaccggcg cgaccgttcc ctccgagtca tcgcgcaggt gccaccatgc cgtctctact
     4561 ggacgaggaa gggacgcgct ctcacggccc tggcggagga tgcggcggaa ccatcgcttc
     4621 agtatgagat ggcgaccggc cgggtgaggc gcctccatgt gcccgagggc ctggtgcccc
     4681 tggtgccttc ccccacggat gacgccatgc tctacttaga gacgcgggcc cccgggcaga
     4741 tgatggtggg cgaagcggag ggcaaccgcc tgcgtctctt cgctcacggc tacttcccag
     4801 cctggtcgcc ggacggcgag agcatcgcct tctacgacaa cccggatgag gaggcgagcc
     4861 tgtgcatgca gagcc
//
LOCUS       DAOSCV010000240         4870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10940_length_4870_cov_3.106334, whole genome shotgun sequence.
ACCESSION   DAOSCV010000240 DAOSCV010000000
VERSION     DAOSCV010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10940_length_4870_cov_3.106334"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(533..2962)
                     /locus_tag="PLY56_07270"
     CDS             complement(533..2962)
                     /locus_tag="PLY56_07270"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ21317.1"
                     /translation="MLLSTAPARAGEANLVLPPLGAQEYTILHIVFASAIVSLLFGYW
                     LVRKVMAVDEGTQAMKDVAAAIHDGAMAYLARQIKTMIWLVIGLAILLFFVYKGVFVH
                     AAEVARAADPTYQEIMSPTALAVGLAFAFILGVAASYGAGFVGMKMAVRGNVRVAAQA
                     LSSYKGALETAFRAGSVAGMFTVGLGLLGATIVFYIFRQEAMKVLLGFGFGGALAALF
                     MRVGGGIFTKAADVGADLVGKVEQGIPEDDPRNAATIADNVGDNVGDCAGMAADVFES
                     YEVTLVAAIILGAAAGPVGSPLAPFVMKLIMYPLLVRAVGVVASIIGILSVRGKDDEN
                     MDPMAPISRGYWIAAGVAVIGFGVITRFFMNDFVADDGTVIAPWYSFFCATLMGIILA
                     LVIERLTEYFTATEKAPVTEIANSTKTGPATVILSGLSVGLESSVWSVVAIGATIIGS
                     VLIFPGSPALAAYGIALSGLGLLTTTGYMLAMDTYGPISDNANGIFEMSGALEGNEGA
                     SRIVAKLDAVGNTTKALTKGLAIATAVIAATALFRSFMADAGLLPPEGMAALSAGAED
                     LLRSGIHLDWPEVFVGMLIGGAVPFLFSSFAINAVSRAAFMLVQEVRRQFREIPGIME
                     GKNKPDYQRCVDISTAAAQKELLGPGILAVFAPILVGFGLGFADPLKGAASLGGFLAG
                     AILSGQLMAVFMSNTGGAWDNAKKKVEDGFLGGKGTDAHKAAVIGDTVGDPLKDTAGP
                     ALNPMIKVMNLVAVLIAPIVIQDISLAARTLVVLMAIAFLAVAVLFSKKGGITTKEAE
                     EVPAKTRVGKA"
     gene            complement(3290..3613)
                     /locus_tag="PLY56_07275"
     CDS             complement(3290..3613)
                     /locus_tag="PLY56_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOJ21318.1"
                     /translation="MPSDEPASPTAKAEGAEPSPGARTEDAASNGEGALFRVEVGAPA
                     TRADAERLASELRQHGFASHVVVEGNIIHVQAGAFRSRRNAERLATRLGDKGYAATIA
                     GGPDE"
     gene            complement(4489..>4870)
                     /locus_tag="PLY56_07280"
     CDS             complement(4489..>4870)
                     /locus_tag="PLY56_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21319.1"
                     /translation="SSCTSGRKRPIPPPTPVRNLLELIRLRVDAAGVGVESIAAKEDH
                     LTVILAPRNRLDDKTVARLWRHPVANRPGWRWVQVRPDRIVVELQGGSALGAAAAVVR
                     ALAEPAPARPSSKGRQGSATRRNP"
BASE COUNT          888 a         1596 c         1578 g          808 t
ORIGIN      
        1 gttccctcca cggagccgag aaggcctccg caccctgcga gggctactcc aagcgccagc
       61 ggcgcgagag cagccagata ccgaaggttc ttcacacttg gtttcaaagc agtgtccccc
      121 tttctctcct gtggaccgtc ggagaggacc agagtcatca ctgagtcacc cggccaggat
      181 cgggttccgt gtttcccctc ctggttgctt ccttccgtcc ggcctcagtg cccgggtcgt
      241 tgcgcaggcg cagcgcccgg atatctatca gtttgactgc ctaggtgcaa gtgcgcgtag
      301 atggcgtctg gctggtgcac gcaccaaccg ctgcaagtcg ttggcactgc tcccgccgca
      361 gggcatcctg ccgtgcggcc cgggggcgtc cctctcgggc gagggtaacg ctataaccat
      421 agagttcgag agggccgcga aggtttcctc tagctgatca aaagcgtgct tttcgagcgt
      481 ggaatatgca gcggggggca ggttagcctg ccccccgcca gtcggttgtc agttaggctt
      541 tcccgacgcg agtcttcgca ggaacctcct cggcttcctt cgtggtgatt ccgcccttct
      601 tgctgaagag gacggccacg gcgaggaacg caatcgccat gaggacgacg agcgtgcgtg
      661 ccgcgaggct gatgtcctgg atgacaatcg gcgcgatgag cacggccacg aggttcatga
      721 ccttgatcat cgggttgagc gccgggccgg ccgtatcctt gagcgggtca cccacggtgt
      781 cgccgatgac ggcggccttg tgggcatccg ttcccttgcc gcccaggaag ccatcctcaa
      841 ccttcttctt ggcgttgtcc catgccccac cggtattgga catgaagacg gccatcaact
      901 ggccactgag gatggcgcca gcgaggaagc cgccgaggct ggcagcgccc ttgagcgggt
      961 cggcgaagcc gaggccgaag ccgacgagga tcggagcgaa gaccgcgagg ataccggggc
     1021 cgagcagctc cttctgagcc gccgcggtgc tgatatcgac gcagcgctga tagtcaggct
     1081 tgttcttccc ctccatgatg ccggggatct cacggaactg gcggcgtact tcctgcacca
     1141 gcatgaacgc cgcgcggcta accgcgttga tagcgaacga gctgaacagg aagggcaccg
     1201 cgccaccgat cagcatgccc acgaagacct cgggccaatc gaggtgaata ccggatcgga
     1261 ggagatcctc ggcgcctgcg ctgagggcag ccattccctc gggcggcagc aggccggcat
     1321 cagccatgaa ggagcggaag agcgccgtgg cggcgataac cgctgtggcg atagcgagac
     1381 ccttggtgag ggccttggtc gtgtttccaa ccgcgtcgag cttggcgacg atgcggctgg
     1441 cgccctcgtt cccctccagc gcgccagaca tctcgaagat gccgttcgcg ttgtcggaga
     1501 tggggccata ggtgtccatg gccagcatgt agccagtggt agtcagaagg cccaggccag
     1561 acagggcgat gccgtaggcc gcgagggcgg ggctgcccgg gaagatcagg accgagccga
     1621 taatggtggc gccaatggcg acgaccgacc acacagagga ctcgaggccg acgctcaagc
     1681 cggagagaat aaccgtagcc gggccggtct tggtcgagtt tgcaatctcg gtgaccgggg
     1741 ccttctcggt ggccgtgaag tactcggtca ggcgctcgat gaccagggcc aggatgatgc
     1801 ccatgagcgt agcgcagaag aaggagtacc acggggcgat cacggtgccg tcgtcagcga
     1861 cgaagtcgtt catgaagaag cgagtgatca cgccgaagcc gatcacagcc acgccagcgg
     1921 cgatccagta accgcggctg atcggggcca tgggatccat gttctcgtca tccttgccgc
     1981 gaacgctgag gatgccgatg atcgaggcca caacgcccac tgcacgaacg agaagcggat
     2041 acatgatcag cttcatcacg aacggggcaa gcggcgagcc caccgggccg gccgcggcgc
     2101 cgaggatgat ggcggcgacc agggtgacct cgtagctctc gaagacgtct gcggccatac
     2161 cggcgcagtc gccgacgttg tctcccacgt tgtccgcgat ggtcgcggcg ttgcgcgggt
     2221 catcctccgg gatgccctgc tcgaccttac cgaccaggtc ggcgccgaca tcggctgcct
     2281 tggtgaagat accaccgcca acgcgcatga agagcgcggc gagggcgcca ccaaagccga
     2341 agccgagcag aaccttcatc gcctcctgtc ggaagatgta gaagacgatg gtggcgccca
     2401 gcaggccaag acccaccgtg aacatgccgg ccaccgagcc agcccggaac gccgtctcga
     2461 gagcaccctt gtaggagctc agcgcctgag cagcgacgcg cacgttcccg cgcacggcca
     2521 tcttcatgcc gacgaagcca gcgccgtaag aagcggccac gcccaggatg aacgcgaacg
     2581 ccaaccccac cgcgagcgcc gtaggcgaca tgatctcctg gtaggtggga tccgcagcgc
     2641 gggcaacttc agcggcgtgc acgaagacgc ccttgtaaac gaagaacagc aggatcgcca
     2701 ggccgatgac cagccagatc atcgtcttga tctggcgggc caggtaggcc atagcgccgt
     2761 cgtggatggc agcggccaca tccttcatcg cctgcgtgcc ttcgtctaca gccatgacct
     2821 tgcggacgag ccagtagccg aagagcagcg agacgatggc cgaggcgaac acgatgtgga
     2881 ggatggtgta ctcttgggcc cccaagggcg gcaggacgag attcgcctcg cctgcacgcg
     2941 ccggggctgt gctcagcagc atccccaaag cggatagagc gaacaaaccg gtcttacccg
     3001 ccgcgcgacc gcgtgtggat cgacgcgcgc ggactaacgt gtgcatggtc ttgtgcgtcc
     3061 cccttctgat ttcggaccag gcctgcgggt tgatcccgct gcctatgctc gagctgcccg
     3121 ggatggtccg cttcctcttc aggcccgtgc cgcaaggaga gggaagcctg ctccgcggca
     3181 tgtacttaag aggtgcgccc catcgcgggc cgcgctccgc gttttacgca gatggccagt
     3241 cacgcccggc gtgatggtct ctgagtggta tgccggcgcc tacgccaagc tactcgtccg
     3301 ggccgccggc aatggtcgcc gcatacccct tgtctcccag gcgggtggca agacgctcgg
     3361 cgttgcgccg gctgcgaaat gccccagcct gaacatggat aatgttgcct tccacaacca
     3421 cgtgcgaggc aaagccgtgt tgacgcagct cactcgcgag acgctcggcg tccgcgcgtg
     3481 ttgctggtgc tcctacctcc acacggaaga gtgccccctc gccgttgctc gcggcatcct
     3541 ctgtgcgcgc gccaggcgat ggctcagccc cctccgcctt tgccgtgggg gaggccggtt
     3601 catcggaagg cacggcatct ctagctggag agatggtcgc cttaggccaa tccctggcag
     3661 gggcggcggg ctgggaaggc agatcgaccg acggcgcggg ctctggggcc ggccggacag
     3721 tgacgctctg gggcgcctct gtgcctgtcg tccccccggt aagcggggtc tccgccacgt
     3781 ggcggctctc ttccgctcga gtctccgggc gcgcctcgcg caccgactcg gtagccggcc
     3841 gaggcttgtg ctctcgggat ccgactctct cgcgatcttc tatcgttggc agggcaacac
     3901 ggttctcagt gagatccgcc tcgtccctgg atacttgcgg gctggtgtca gccgggctgc
     3961 gcgacgggta tacgcggggc gcaatccaat acaagctgac aaagaacgaa gccaaagatg
     4021 caacaacaag aatactgccc cacttgaccc aagcgagcgc cgtttcatcc ctgccacggc
     4081 ccctgcgagg acggtgcttg ccagacaccc tctccacttc gggcatacca tacccccgtc
     4141 cacgacgtga tccgaaggac ctcgccggat cgccaggagg agccgccgca catcgcgaaa
     4201 ccatgtgcac gacgcatccc cgcagggaat gcctgacgca cctgaggtcc gctacggact
     4261 cgattcactc ttcggccaac aaaccatacc gcgtcagtga aacaaccccg cgcccccccc
     4321 atgggcagac gcaagcacgc aagtcacccc gcgccgtggc atgctcaccc gccggcgagc
     4381 cctctctgga ccaaaaaggg agagacgcaa gcagctttgc ggcaggcgta tctggatgct
     4441 tcccagacac atatgtcgcg gagagtatac aatgtttcca tgcgcctgtc aagggttccg
     4501 gcgtgtggca gaaccctgtc tccccttgct ggaggggcgt gcgggggccg gttccgcgag
     4561 tgctcgaacc acggcagccg ccgcccccag cgccgatcct ccctggagtt cgacaacgat
     4621 ccggtcgggg cgcacctgca cccaccgcca ccccgggcgg ttggcgacgg gatggcgcca
     4681 tagccgggcg accgtcttgt catctagccg gttgcgcggc gccaggatca cggtgaggtg
     4741 atcctccttg gcggcgatgc tctccacgcc aacaccggcg gcatccacgc gcaggcggat
     4801 cagctccagc aggttgcgca cgggcgtcgg tggcgggatc gggcgtttcc tgcccgacgt
     4861 gcagctggag
//
LOCUS       DAOSCV010000241         4869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10941_length_4869_cov_4.018488, whole genome shotgun sequence.
ACCESSION   DAOSCV010000241 DAOSCV010000000
VERSION     DAOSCV010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10941_length_4869_cov_4.018488"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLY56_07285"
     CDS             <1..521
                     /locus_tag="PLY56_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HOJ21320.1"
                     /translation="TLLPNAVRTALCRSTGVKVYVCNVMTQPGETTGYTASDHVRALQ
                     KHGAGDCFDFVLVNTEPPPPDAAERYRRTGAGPVAADLDAIAKLGYIPVAAPLLNMDQ
                     FVRHDPRKLANALRWIWGGLADRASFPTQFASDRGEIRPPIAGEIEPPVAEAGARPAE
                     PVIGVAMAGDSR"
     gene            complement(544..1833)
                     /locus_tag="PLY56_07290"
     CDS             complement(544..1833)
                     /locus_tag="PLY56_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21321.1"
                     /translation="MKRIFRWVAGARLPGEGMHPLSNPTHHRITQSAIAALPGEDREF
                     LAPEADLLIWTYCGFPDMNWHWYGTFAEDPSIPFDRRLPDIRREWEISRYCAWNSLTR
                     EGRWYPHWPPDALEATADHFSRAVKTLAEGGYHDALRILGAAVHYAEDCGSPAHAGHI
                     DPPLHLPMERIDRPEMEAAIAIPGYTPRLLGSALETACDGIRERGQKVVAFAETAAGK
                     IRELCEAGEQHEAQSLMVPCAQECARYVADLLHTFCVLAEHARPETPIAPHGCNLIAN
                     ADLSESDDVPQVPRGWYIHWNDLRDRNGRAGLVASDHSPALMVESPPEQGIHWRTPWP
                     RAARVRLGMRIHLAIRYRTEGPQGSNRCSIRFYTGNTTLVGIFCTDLLPPSEEWCDVG
                     LSAEVPEGASWARVSLEASGGEGRILFRDPRLVRLNP"
     gene            1977..2738
                     /locus_tag="PLY56_07295"
     CDS             1977..2738
                     /locus_tag="PLY56_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ21322.1"
                     /translation="MLETVDGFLLDLDGTCYLGDRAIEGAPEFVARCRARGKRVLWLT
                     NNCSRTATEYAEKLCRLGFEATPRDIFTSGDATIQVLGSEGVTRVFLLGTPSLEEEFR
                     QAGITLTATDPERVVVGFDLGLTYDKLKEACRLIRSGVPWVATHPDLNCPTPEGPIPD
                     CGAICALIAASTGARPQVIGKPEAGMAEAAAMRIGIPRQRLAMVGDRLYTDIRMAREN
                     EMTAILVLSGEATREDVAQSPFQPHHIVPSLAFLL"
     gene            2875..4206
                     /locus_tag="PLY56_07300"
     CDS             2875..4206
                     /locus_tag="PLY56_07300"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOJ21323.1"
                     /translation="MQDNDREYVLKTAKEQNVRFIRLWFTDILGFLKSVAITVDELEE
                     ALEEGMGFDGSSIEGFARIDESDMIAMPDPSTFQILPWRPREEAVARMFCDIRTPEGE
                     PYEGDPRWVLRRALKRAADMGYTFYVGPELEYFYFKNARGIPEGLDKGGYFDLTPLDV
                     ASNLRRETVLTLEEMGIGVEYSHHEVAHSQHEIDLRYTDALTMADNAMTYRLVVKEVA
                     MRHGIHATFMPKPIFGQNGSGMHTHQSLFRGDTNAFFDPTAPYHLSKIARHYIAGLLR
                     HIPEVTLVLNQWVNSYKRLVPGYEAPIYLSWAVKNRSDLIRIPQYKPHKESAMRVELR
                     SPDPACNPYLAFAVMLACGLEGILNEYPLGDPVECNVYEMTAEERDARGIRTLPEDLS
                     AAVQLAEKSDLLRRTLGNHVFEKLIENKKIEWERYRAQVTTWELDQYLPVL"
     gene            complement(4201..>4869)
                     /locus_tag="PLY56_07305"
     CDS             complement(4201..>4869)
                     /locus_tag="PLY56_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21324.1"
                     /translation="RPSLLVLRLLCCACGHSLAFVLAPAFAAMAVLQDKRSRMWEDLL
                     LTRLASEEIVVATLAAALQIPALLLTVQAALVLLALIGGLWHTDAGTSWQECLFRLVV
                     MLPALVTGAVAAASFLVVGGLIGIRAGLHFGDALGGVASAILGTLAAGVLTVAAGILT
                     MPRMVSPIQALAPPRGLLLSPVWSVELWAAMSLEMALLALLNALLASLLWRACVRAVR
                     PASS"
BASE COUNT          888 a         1638 c         1548 g          795 t
ORIGIN      
        1 ctacgcttct gcccaacgcg gtccgcaccg ccctctgccg cagtaccgga gtgaaggtat
       61 acgtctgtaa cgtcatgacc cagccagggg agacgaccgg ctacaccgcc agcgaccacg
      121 tgcgcgcgct ccagaagcac ggcgcgggtg attgcttcga ctttgtgctg gtcaacacgg
      181 agccgccgcc acccgatgcc gcggagcgtt accggcggac aggggccggc ccggtcgcgg
      241 cggatctcga tgccatcgcc aagctgggct atatcccggt ggccgcccct ctcctgaata
      301 tggaccagtt cgtccggcac gatcctcgca agctggccaa cgccctgcgg tggatctggg
      361 gggggctggc agaccgagcg tccttcccca cccagttcgc ctcagacagg ggtgagatcc
      421 ggccgcccat cgcgggcgaa atcgagccgc ctgtggcgga agcaggcgcc aggccggctg
      481 aacccgtcat cggggtcgca atggcgggag actctcggta gcgcgcgctc gaaaggggcg
      541 tggttaggga ttcaggcgga cgaggcgagg atcccggaaa aggatcctcc cttccccacc
      601 ggacgcctcc agggaaaccc gtgcccagga ggcgccttcg ggcacctcgg cgctcagccc
      661 cacatcacac cactcctccg acggcgggag caggtccgtg caaaagatcc cgaccaacgt
      721 ggtgtttccg gtgtagaagc gaatcgaaca gcgattggag ccctgcggcc cctccgttcg
      781 atagcggatc gccaggtgga tgcgcatccc caggcgcacg cgcgcggcgc gtggccacgg
      841 ggttcgccag tggatcccct gctccggggg agattccacc atcaatgccg ggctgtggtc
      901 actcgccacc agaccagccc gaccgttgcg atccctgagg tcgttccagt gaatgtacca
      961 gccgcgcggc acctgtggca catcatcgct ttcagacaga tcggcgttgg caatcaggtt
     1021 gcagccatgc ggtgcgatgg gcgtctccgg gcgcgcgtgc tcagccaaca cgcagaaggt
     1081 gtggagcagg tccgcaacat agcgcgcgca ctcctgcgcg cagggcacca tcaagctttg
     1141 ggcctcgtgc tgctcccccg cctcgcacag ctcccggatc ttcccggcag ccgtctcggc
     1201 gaacgccacc accttctggc ctcgctcgcg gatgccgtcg cacgccgtct ccagggcact
     1261 tcccagcagt cgcggggtgt atcccgggat ggcgatagcg gcctccatct ccggacggtc
     1321 gatccgctcc atggggaggt ggagtggcgg gtcgatatgc cccgcatgcg cgggcgagcc
     1381 acaatcctcg gcatagtgaa cggctgcccc gaggatgcga agtgcgtcgt gatagccgcc
     1441 ctccgcaagc gtcttcacgg cgcgggagaa gtggtcggcc gtggcttcca gcgcatccgg
     1501 gggccagtgg ggataccatc gaccctctcg cgtgagcgag ttccaggcgc aataacgcga
     1561 gatctcccat tccctgcgga tatccgggag acgcctgtcg aaggggatgc tggggtcctc
     1621 ggcaaaggtc ccataccagt gccaattcat atcggggaag ccgcagtagg tccagatgag
     1681 gagatccgcc tcgggcgcca ggaactctcg atcctcgccc ggaagcgccg cgatagccga
     1741 ttgcgtgatg cggtggtgcg tcggatttga gaggggatgc atgccttcgc ctggtagccg
     1801 cgcgcctgca acccatcgga agatccgctt caccttctcc cccattccaa ccggccggcc
     1861 tggcgggaac cggccccgat tgctacctac ttcgtcgccg ggccatgcct gcccttctcg
     1921 cgccctggcg ccagccggaa ggattcccgg cggccgcatc caaccctctc cgtgctatgc
     1981 ttgagactgt ggacggcttc ctgttggacc tggatggaac ctgctatctc ggggatcgcg
     2041 cgattgaggg agcgcccgag tttgtagccc gctgtcgggc gcgcggcaag cgcgttctct
     2101 ggctcaccaa caactgctcg cgcaccgcaa ccgagtacgc ggagaagctg tgccgccttg
     2161 gcttcgaggc cacgccacgc gatatcttca cctccggcga cgcgacgata caggtgctcg
     2221 gaagcgaggg cgtcacccgc gtcttccttc ttggcacccc cagccttgag gaggagttcc
     2281 gccaagccgg catcaccctc acggcgaccg atccagagcg agtcgtcgtg ggcttcgacc
     2341 tgggtctcac ctacgacaag ctgaaggaag cgtgccgcct catccgttcc ggcgtgccct
     2401 gggtcgccac ccatcccgac ctcaactgcc caacccccga gggccctatc cccgattgcg
     2461 gcgccatttg tgctttgatc gccgcgtcca ccggcgcccg cccgcaagtg atcgggaagc
     2521 cggaggcggg gatggcggaa gcagccgcga tgcgcatcgg catccccagg cagcggctcg
     2581 ccatggtagg tgaccgtctc tacaccgaca ttcgtatggc ccgggagaac gagatgacgg
     2641 cgatcctcgt cctctccggg gaggccaccc gggaagacgt cgcgcagtcg cccttccaac
     2701 cgcaccatat tgttccttcg ctcgcattcc tcctctgagg ggcccagatg gttcaccgtg
     2761 cggtgacgct tccgtaacgt ggttgttaca atccggcggt ataatgagtt gacagatcgc
     2821 caggcaaggc ggcgcccggt gtcgcctggc ctgccgcgca cagggagagt tgccttgcag
     2881 gataacgacc gtgagtacgt actgaagacg gccaaagagc agaacgtccg gtttattcgg
     2941 ctgtggttca ccgatattct cggcttcctg aagtcggtcg cgatcacggt ggatgagctt
     3001 gaggaggccc tggaagaggg aatgggcttt gatggctcgt ccatcgaggg gttcgcgcgc
     3061 atcgacgaga gcgacatgat cgccatgccg gatccctcca cctttcagat cctgccatgg
     3121 cgaccgcgcg aggaggccgt cgcccgcatg ttctgcgaca tccgcacccc ggagggggag
     3181 ccgtacgagg gcgatcctcg ctgggttctg cggcgggcac tcaagcgcgc ggcggacatg
     3241 ggctacacct tctacgtcgg ccctgaacta gagtacttct acttcaagaa cgcgcgcggc
     3301 attccggagg gactcgataa gggaggctac ttcgatctga cccccctcga tgtggcgagc
     3361 aatctgcgcc gcgagacggt cctcaccctc gaggagatgg ggatcggcgt ggaatactcg
     3421 caccacgagg tcgcgcatag ccagcatgag attgacctcc gctatacgga cgcgctgacc
     3481 atggcggaca acgccatgac ctatcgcctg gtggtgaaag aggtggcgat gcggcacggg
     3541 atccacgcca cctttatgcc caagccgatc tttggccaga acggcagcgg gatgcacacg
     3601 caccagtccc tctttcgcgg cgacaccaac gccttctttg atcccaccgc cccctaccac
     3661 ctctcgaaga tcgcccggca ctacatcgcc ggcctgctgc gccatatccc agaggtgacc
     3721 ctcgtcctca accagtgggt caactcctac aagcgtctgg tccccggcta cgaagccccg
     3781 atctacctct cctgggcggt gaagaatcgt tctgatctga tccgcattcc gcagtacaag
     3841 ccccacaagg agagtgcaat gcgcgtggag ctacgctcgc ccgacccggc ctgcaatccc
     3901 tacctggcat tcgccgtcat gctcgcctgc ggactcgagg gaattctgaa cgagtatccc
     3961 ctgggcgatc cggtggaatg caatgtgtat gagatgacag ccgaggagcg ggatgcgcgc
     4021 ggcatccgca ccctgccaga ggacttgagc gccgcggtcc agctcgccga gaagagcgac
     4081 ctcctgcggc gcaccctggg caaccatgtc ttcgagaaac tgatcgagaa taagaagatc
     4141 gagtgggagc gttatcgggc acaagtgacc acgtgggagc tggaccagta tctgccggtg
     4201 ttatgagctc gctggacgca cggcgcgaac gcaggccctc cagagcaggc tggcgaggag
     4261 ggcgttgagg agcgccagga gcgccatctc cagcgacata gcggcccaca gttcgacact
     4321 ccacacgggc gatagcaata gcccccgggg cggggccagg gcctggatcg gactcaccat
     4381 ccgtggcatc gtcaggattc cggcggcgac cgtcaacact ccggcggcca gcgttccaag
     4441 gatggcgctc gccacgccgc caagagcgtc gccgaagtgc aaccccgcgc ggatgccgat
     4501 cagcccaccc acgacgagga aactcgcggc tgcgacggcg cccgtgacga gcgcgggcaa
     4561 catcacgacc agcctgaaca gacactcctg ccaggaggtg ccggcgtccg tgtgccagag
     4621 cccgccgata agagcaagca acaccagggc ggcctgcacc gtcagcagga gggccgggat
     4681 ctggagcgcc gccgccagcg tagccacgac gatctcttcg gaggcaagcc gggtcagcag
     4741 gagatcttcc cacatccggg agcgcttgtc ttggagaacg gccatcgccg cgaacgcggg
     4801 cgccagcaca aaggcgagcg agtgcccgca cgcgcagcac aagagcctca acaccagcag
     4861 cgaggggcg
//
LOCUS       DAOSCV010000242         4865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10957_length_4865_cov_3.486694, whole genome shotgun sequence.
ACCESSION   DAOSCV010000242 DAOSCV010000000
VERSION     DAOSCV010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10957_length_4865_cov_3.486694"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLY56_07310"
     CDS             <1..792
                     /locus_tag="PLY56_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOJ21325.1"
                     /translation="KIAHAHGVPLVIDNTFPSPYLVKPIRWGANVVVHSTTKFIGGHG
                     TSIGGIIIDGGNFNWANGRFPAFVDPDPSYHGLKIWETFGDFPGAGNIAFVMKARLQL
                     LRDLGPSMSPFNAFLFLQGLESLHLRMQRHSENALAVARWLEKHPCVTWVSYPGLESH
                     PQHAIAKKYHQGGFGAMIGFGIKGGVEAGRRLIDHVKLFSHLANVGDAKSLIIHPAST
                     THQQLSEEEQLASGVTPDFVRLSIGLEHVDDIIADLDQALAAACA"
     gene            821..1987
                     /locus_tag="PLY56_07315"
     CDS             821..1987
                     /locus_tag="PLY56_07315"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOJ21326.1"
                     /translation="MAAKPEPGRDDRSVGLVETQYLTFAEPPDEMVLTSGAKLGPITL
                     AYETYGQLNPARSNAILVLHALSGDAHVAGRHTPEDRKPGWWDEMVGPGKALDTNRYF
                     VLCANVIGGCKGSTGPNSINPATGKPYGLRFPVVTIQDMVAAQVKLVDHFGIDQLYAV
                     IGGSMGGMQALQWAVAYPDRVRHCIPLATTHRLTAQGIAFNAVGRQAIMSDPGWAGGD
                     YYGGPGPTRGLAVARMVGHVTYLSEEAMRLKFHRKLRPDQESYHYAFENEFQVETYLE
                     YHGKGFVGRFDANSYLYITKAMDYFDLGADWGGLGNALARADARFLIVSVSSDWLFPT
                     SRSRLLVQHLQAAGKDVTFVELQSPYGHDAFLLEVEALTALIEAFFAADPPRES"
     gene            1996..2712
                     /gene="metW"
                     /locus_tag="PLY56_07320"
     CDS             1996..2712
                     /gene="metW"
                     /locus_tag="PLY56_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOJ21327.1"
                     /translation="MTDGSSKPAAFGEVGAALHKRGDLQLITELVPHGARVLDLGSGD
                     GLLLEHLRREKGADGRGIERDPQAILACMRRGIPVVQADLDAGLADFPDRSFDVVIIS
                     HTLQQVARPRHLLLEAGRVGRQVIVGFPNFGHWQIRSALLLTGRMPKSPMLPYEWYDT
                     PNIHLLTLADFEDFCAREGFGILRRMVLRYPCWRQVRLLPGLRAAYALYLLDATGVHS
                     DHGAGASRQEAAEQILVVEE"
     gene            2746..4683
                     /locus_tag="PLY56_07325"
     CDS             2746..4683
                     /locus_tag="PLY56_07325"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ21328.1"
                     /translation="MDPIRIDRMSDEAPVQWAVSELQKYLARATSRRVEVASGATGAG
                     PAIRVGTFEAFSEGVPARASDHPYDDEIFIEAGPGGAVIAGANPRSVVLAAYRYLTEL
                     GCRWVRPGADGEYIPRIDDPFATCVAVHERPSYRHRGVCIEGAVSLEHVCDMVDWLPK
                     VGMGAYFIQFREAYQFFVRWYHHEFNPLQPKEAFTIEDARAYTRRIAEEIEKRGMILH
                     MVGHGWTCDPFGVAGLGWFPYTDPVPESYYQHVAEVNGKRDLWGGIPLNTNLCYGNPE
                     TRRIVVQAVADYAAEHPEVDIIHLWLADGSNNQCECPLCRDHLPSDLYVKLCNEVDAE
                     LTARGLPTKIVFLIYVDLLWPPREEKLANPDRFILMFAPITRTYSSSFTAGATGEGTI
                     PPFERNKLKFPRTPEGNLAFLRGWQKSFDGDSFDFDYHLMWDHFRDPGAWALARVLHE
                     DIRGLRSIGLNGFISCQEQRTFLPSGLLMAVMARTLWNRDASFDEIANDHFRAAFGAD
                     WEKAKEYTQRLSELFDPPFLRGERDEAGRKDAAAKLAQVPALVTEFMPVIDRNLNLAV
                     QCHAQSWRYLKDHAEICLRMAPAAEALARGDKEQARVLARRLLDVVRHLEPQVHPVFD
                     VALFIRTIGGALGLTAEEMAR"
BASE COUNT          821 a         1602 c         1603 g          839 t
ORIGIN      
        1 aagattgccc acgcgcatgg cgttcccctg gtgatcgaca acactttccc ctcgccttac
       61 ctggtgaagc cgatccggtg gggcgcgaac gtagtggtcc actcgacgac gaagttcatc
      121 ggggggcatg gcacgtccat cggcgggatc atcatcgatg gcggcaactt caactgggcc
      181 aacgggcgct tcccggcttt cgtggacccg gatccgagct accacggcct gaagatttgg
      241 gagacgttcg gagacttccc gggggctggc aacatcgcct tcgtcatgaa ggcacgcctc
      301 cagctgttgc gtgacctggg cccgtcgatg agcccattca acgccttcct cttcctccag
      361 ggcctggagt cgctgcacct gcgtatgcag cggcacagcg agaacgcgct ggccgtggcg
      421 cgctggctgg agaagcaccc gtgcgtcacc tgggtgagct accctggcct ggagagccac
      481 ccgcagcacg cgatcgcgaa gaagtatcac cagggtggct tcggcgcgat gatcggcttt
      541 gggatcaagg gcggcgtcga ggccggccgg cggctgatcg accacgtcaa gctcttctcg
      601 cacctggcca atgtgggcga tgccaagtcg cttatcatcc acccggcgag caccacgcac
      661 cagcagcttt ccgaggaaga gcagctggcc agtggggtga cgcccgactt cgtgcgcctc
      721 tccatcggtc tggagcacgt ggacgacatc atcgcggacc tggaccaggc gctcgcagcg
      781 gcgtgcgcgt gaaacgtggc ccggaaacct gagaggacct atggctgcca aacctgaacc
      841 gggccgggac gaccgctccg ttggcctggt ggagacgcaa tatctgacgt ttgccgagcc
      901 gccggacgag atggtactca cctccggcgc aaagctgggt ccgatcacgc tggcctacga
      961 gacgtatggc cagctcaacc ccgctcggag caacgcgatc ctcgtgctgc atgctctctc
     1021 cggcgacgcg cacgtcgccg gccggcacac gcccgaggat cgcaagcccg ggtggtggga
     1081 cgagatggtg ggtcccggca aggcgttgga taccaaccgc tactttgtcc tatgcgccaa
     1141 cgtgatcggc gggtgcaagg gctccaccgg gccaaacagc atcaaccccg ccaccggaaa
     1201 gccctatggg ctgcggtttc ccgtggtgac gattcaggat atggtcgcgg cgcaggtgaa
     1261 gctggtcgat cacttcggga tcgaccagct ctacgccgtg atcggcgggt cgatgggggg
     1321 aatgcaggcg ctgcagtggg cggttgccta tccggatcgc gtgcgccact gcatccccct
     1381 ggccacgacg caccgtctga cggcgcaggg gatcgccttc aacgccgtcg gccggcaggc
     1441 gattatgtcc gacccggggt gggccggcgg cgattactat ggcggcccgg ggccgacgcg
     1501 cggcctagcc gtggcgcgca tggtggggca cgtcacctac ctcagcgagg aggcgatgcg
     1561 cctgaagttc caccgcaagc tgcgtcctga tcaggagagt taccactacg ccttcgagaa
     1621 cgagttccag gtagagacct acctggagta tcacggcaaa ggcttcgtcg ggcggttcga
     1681 tgccaacagc tacctctaca tcacgaaggc gatggactac ttcgatctgg gcgccgattg
     1741 gggcgggctg ggcaacgcgc tggcgcgggc agatgcgcgc ttcctgatcg tctcggtcag
     1801 ctccgactgg ctcttcccca cgagccggtc gcgcctgctc gtgcagcacc tgcaggcggc
     1861 cggtaaagac gtcaccttcg tggagctcca atcgccctat gggcacgacg cgttcctgct
     1921 cgaggtggag gcgctgacgg cgctcattga ggcgtttttc gcggctgacc cgccccgaga
     1981 aagctagtga aaccaatgac ggacggatcc tcaaaaccag ccgcgttcgg cgaggtgggc
     2041 gctgccctcc acaagcgcgg tgacctgcaa ctgatcacgg agctcgtgcc tcatggcgcg
     2101 cgagtgctcg acctcgggtc gggcgatggg ctgctcctgg aacacctgcg ccgggagaag
     2161 ggcgcggatg gccggggaat cgagcgcgat ccccaggcga ttctcgcttg catgcgccgg
     2221 ggcatccccg tcgtccaggc cgacctggat gcaggcctgg cggactttcc cgaccggtcg
     2281 ttcgatgtcg tgatcatcag ccacacgctc cagcaggtgg cacggccgcg ccacctgctg
     2341 ctggaggcgg gccgcgtggg caggcaagtg atcgtcggtt tcccgaactt cgggcactgg
     2401 cagatccgct ccgcgctgct cctcacgggg cgcatgccca agtcgcccat gcttccctat
     2461 gagtggtatg acacccccaa catccacctg ctgactctgg ccgacttcga ggatttttgc
     2521 gcccgcgaag gcttcggcat cctgcgccgc atggtgttgc gctatccttg ctggaggcag
     2581 gtgcggctgc tccccgggct ccgcgccgcc tacgcgctct atcttctcga cgccacggga
     2641 gtacactccg accacggcgc tggcgcttcg cgacaggaag ccgcggagca gattctcgtc
     2701 gtcgaagagt aggggagggt gccccgcgtg atcgggagga gagagatgga ccccattcgt
     2761 atcgaccgca tgagcgacga agcccccgtg cagtgggccg tttcggagct gcagaagtat
     2821 ctagcccgcg ccaccagcag gcgggtggag gtcgcgagtg gcgcgaccgg cgccggcccg
     2881 gcgattcgcg tcggcacctt cgaggcgttt tcagagggcg tgcccgcgcg cgcttccgac
     2941 cacccctatg acgacgagat cttcatcgag gcggggcccg gcggtgctgt gatcgccggt
     3001 gcgaacccgc gcagcgtggt cctggccgcc taccgctatc tcaccgagct cggctgccgg
     3061 tgggtgcgcc ccggtgccga tggtgagtac atcccccgga tagacgaccc cttcgccaca
     3121 tgcgtggccg tgcatgagcg cccctcctac cggcaccgcg gcgtctgcat cgagggcgcg
     3181 gtgagcctgg agcacgtgtg cgacatggtc gactggctcc cgaaggtggg gatgggtgcc
     3241 tacttcatcc agttccgcga ggcgtaccag ttcttcgtgc gctggtacca ccacgagttc
     3301 aacccgctgc aacctaagga ggcgttcacc atcgaggatg cccgcgccta cacgcgccgc
     3361 atcgccgagg agatagaaaa gcgcgggatg atcctgcaca tggtgggcca cggctggacg
     3421 tgcgatccct tcggcgtggc cggccttggc tggttcccct acaccgaccc ggtacccgag
     3481 agctattatc aacacgtggc cgaggtgaac ggcaagcgcg acctgtgggg cgggatcccg
     3541 ctgaacacca acctttgcta cggcaacccc gagacgcgcc ggatcgtggt ccaggccgtc
     3601 gccgactacg cggcggagca cccggaggtc gatatcatcc acctatggct ggccgatggg
     3661 agcaataacc agtgcgaatg ccccctgtgc cgcgaccacc tgccgtccga cctttacgtg
     3721 aagctgtgta acgaggtgga cgccgagttg accgcgcgcg gcctgcccac caagatcgtc
     3781 ttcctgatct acgtggacct tctctggcca ccccgggagg agaagctggc gaacccggac
     3841 cgcttcatcc tcatgttcgc gccgatcacg cgcacctact cctcttcttt caccgccgga
     3901 gcaaccggcg agggcaccat cccgccgttc gagcggaaca agctgaagtt cccgcgcacg
     3961 ccggagggga acctcgcctt cttgcgcggc tggcagaaga gcttcgatgg agacagcttc
     4021 gattttgact atcacctgat gtgggatcac ttccgggatc cgggtgcctg ggcactggcg
     4081 cgcgtgctcc acgaggatat tcgtggcctg cgctccatcg gcctgaacgg gttcatcagc
     4141 tgccaggagc agcgcacctt cctccccagc ggccttctca tggcggtgat ggcgcgaaca
     4201 ctgtggaacc gcgacgcttc gttcgatgag attgccaacg accacttccg agccgctttc
     4261 ggcgccgatt gggagaaagc gaaggagtac acgcagcgcc tctccgagct gtttgaccca
     4321 ccgttcctgc ggggcgagcg tgacgaagcc ggccggaaag acgccgccgc gaaactggcc
     4381 caggtgccgg cgctggtgac cgagttcatg ccggtgatcg ataggaacct caaccttgcg
     4441 gtgcagtgcc acgcgcaatc gtggcgctat ctgaaggacc acgcggagat ctgcctccgg
     4501 atggcgcctg ccgcagaagc cctcgcgcgg ggcgataagg agcaggcgcg cgtgcttgcg
     4561 cggcgcttgc tcgacgtagt tcgccacctg gagccgcagg ttcaccccgt cttcgacgtg
     4621 gcgctcttca tccggaccat cggcggggcg ctcggattga cggcggagga gatggcgcgg
     4681 tagcccgcgc atggcttgcc tccacggcgc tatgtgccgt accagatgat gagtgtgctc
     4741 aggttctcgt actgactgag gaagcgcagc cgcagcctct ccgtgcgcat ggagagggcc
     4801 accgaggtgc cgtgggggcg gtccaccacg cggatcgcct gacgcacatc ctccgcatcc
     4861 acccg
//
LOCUS       DAOSCV010000243         4865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10958_length_4865_cov_3.258420, whole genome shotgun sequence.
ACCESSION   DAOSCV010000243 DAOSCV010000000
VERSION     DAOSCV010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_4865_cov_3.258420"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLY56_07330"
     CDS             complement(<1..428)
                     /locus_tag="PLY56_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21329.1"
                     /translation="MSSDAPSEALSEGLNAALDTLGVLLLEYGRNGFDTDEMTARQLH
                     IRCEQWARHLLSGGPKPGEEAAKQGLPIHQRDWRGVCQFFVAHRREECRYINRSSVDL
                     RQSLWTLIQVLDATVAQDEEADQLVQAELEQLKRAAESDSA"
     gene            complement(572..2167)
                     /locus_tag="PLY56_07335"
     CDS             complement(572..2167)
                     /locus_tag="PLY56_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016949876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOJ21330.1"
                     /translation="MNQLLKEIQRRRTFAIISHPDAGKTTLTEKVLLYAGAVRLAGSV
                     KARKGQRHTASDWMEMEQERGISISSTVLQFDYGDYHINLLDTPGHQDFSEDTYRVLT
                     AVDNAVMVIDSANGVESQTRKLFEVCQRRRVPVFTVINKMDRPGKEPLDLLGEIEDVL
                     GIATVPLNWPIRANGVFVGVYDLRERKVHLFERTHHGSHPAPVQVTDLQDPTLESLLG
                     PQPLQQLRDEVELIEAAGDCFDRERVQRGELTPVFFASALTNFGVQLFLDAFLEMAVG
                     PAPRVSNDRVVPPDHPHFSGFVFKLQANMDRRHRDRLAFVRICSGRFERDMVVTHVRT
                     GKKIRLTRTLKLFAQERESLDEAYPGDVVGLINPGIFSIGDTLCTGERVEFREIPRFP
                     PEHFATLSLPEVGQRKAFQKGLDQLHEEGLVQIFYPVGDVMRQPILAAVGPLQFEVLV
                     NRLENEYSVNVTLHPLPHQHALWLEGDAAAIQGMRLVPGCMRVEDCEARPVLLAPDDW
                     SVRYMTEKNPEVVLHRMPVTTQE"
     gene            3053..4222
                     /locus_tag="PLY56_07340"
     CDS             3053..4222
                     /locus_tag="PLY56_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21331.1"
                     /translation="MEALCLALALCWGAAAGMPPEGIMEQEAARVARAFLGDPTIALP
                     KPRMVGPGDDPSFSYELPDGRTVAVNRSTGRVEWYYDRSAELDQRPAVLVSEADTERE
                     IRRFAQEHYPDVLERHLVVRANSSGKNVWVIYIERIPENGALNRMGSLLIEANRTTGR
                     VASVLDKYRPLPPEARRKPNVSRKAAIDTAIGATQFLRVYGRPEVELAWTRPIFADGP
                     ALVWAVSFKGYLGRNRANSVTVWVDALKGGVVGEPAYGVAYFVAEENQCVLHQDWGML
                     PAIPIVVLTLRPRVDEGKLWMPVRFAELFDLKVALEGDTVSLSGKDGECVILARLPVR
                     RWEEEEYLPAEEMLKAVEKQVWEVKADVAHASLLVGVHSAKPLERALALKLGRRP"
     gene            4459..>4865
                     /locus_tag="PLY56_07345"
     CDS             4459..>4865
                     /locus_tag="PLY56_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21332.1"
                     /translation="MKKVRIGVLGVGRGAGLARSARLAPSVELAALCDMSEERLAKAS
                     ATMGVQTTYTSYEAMLDSDVDAVLVASPMPLHVKHAVQALRAGKHVLSEVTAATSIEE
                     CWELFEAVRDSGLKYMLAENYCYMRPWSIVLNMV"
BASE COUNT          866 a         1462 c         1708 g          829 t
ORIGIN      
        1 gctgaatcgc tttcggccgc tctcttcagt tgctccaact ccgcctgcac gagttggtcg
       61 gcctcctcat cctgagcgac ggtggcatcg agcacctgga tgagagtcca tagcgactgc
      121 cgcagatcca cggaggagcg gttgatgtag cggcactcct cgcggcgatg ggcgacgaag
      181 aactgacata ctccgcgcca atcgcgctgg tggatgggca gcccctgctt tgcggcctcc
      241 tcccctggct ttggcccccc cgagaggagg tgccgagccc actgctcgca gcggatatgc
      301 agctggcggg ccgtcatctc atcggtgtcg aagccattgc gcccatactc caagaggaga
      361 acgccaagcg tatccagtgc ggcgttgagg ccctcggaga gggcttcaga aggcgcgtcc
      421 gatgacaccg ctggcgatgc ctcttctgct ggcgccgagc tctctctctt gcggttccac
      481 caccccatca tgtcgggcct ttccaaagat aactatacca gattccggcc cgtagggcaa
      541 ccggatgcct gcctgccacg ggcttcactg gttactcctg cgtcgtgacc ggcatccggt
      601 ggagcacgac ctcggggttc ttctcggtca tatagcgcac ggaccagtca tccggggcca
      661 ggagcacggg ccgcgcctcg caatcttcga cgcgcatgca gcccggcacg aggcgcatcc
      721 cctggatcgc cgcggcgtcg ccttccagcc agagcgcgtg ctggtgcggc agcgggtgca
      781 gcgtgacgtt cacggagtac tcgttctcca ggcggttgac cagcacctca aactgcagcg
      841 ggcccaccgc ggcgaggatc ggctggcgca tcacgtcgcc gaccggatag aagatctgca
      901 ccaggccctc ctcgtggagc tgatccaggc ccttctgaaa cgctttgcgc tggccaacct
      961 ctggcagcga cagggtggca aagtgctccg gcgggaagcg agggatctcg cggaactcca
     1021 cgcgctcgcc agtgcatagt gtgtcgccaa tggagaagat gccggggttg atcaggccca
     1081 cgacgtcgcc gggataggcc tcatcgagcg actcgcgctc ttgggcgaac agcttcagcg
     1141 tgcgcgtgag ccggatcttc ttgccggtgc gcacgtgcgt caccaccata tcgcgctcga
     1201 aacgccccga gcagatgcgc acaaaggcga gacggtcgcg atggcggcgg tccatgttcg
     1261 cctggagctt gaagacaaac ccggagaagt gcgggtgatc cggcggcacc acgcggtcgt
     1321 tcgagacccg gggggccggt ccgacggcca tctccaggaa cgcatcgagg aagagctgca
     1381 cgccgaaatt ggtgagcgcg ctggcgaaga agacgggggt cagctcgccg cgctgcacgc
     1441 gctcgcggtc gaagcagtcg cccgcagcct cgatcagctc cacctcgtcg cgcaactgtt
     1501 gaagcggctg ggggcctagc aggctctcca gcgtcggatc ctggaggtcg gtcacctgca
     1561 ccggggcagg atggctgccg tgatgggtgc gctcgaagag gtgcaccttg cgctcgcgca
     1621 gatcgtagac gcccacaaag acgccgttcg cgcggatcgg ccagttgagt ggcacggtgg
     1681 cgataccgag cacgtcttcg atctcgccca gcaaatcgag cggctccttg cccgggcggt
     1741 ccatcttgtt gatcacggtg aagaccggca cgcgccggcg ctggcatacc tcgaacagct
     1801 tgcgcgtctg cgactcgacg ccgttggcgc tgtcgatcac catcaccgcg ttatccacgg
     1861 cggtgagcac gcgataggta tcctcgctga aatcctggtg gccaggcgta tcgagcaggt
     1921 tgatgtgata atcgccgtaa tcgaactgaa gcaccgtcga ggagatggag atgccgcgct
     1981 cctgctccat ctccatccag tcggacgcgg tatgccgctg accctttcgc gctttgacgg
     2041 acccagccag gcgcaccgcg ccggcgtaga gcaacacttt ctccgtcagc gtggtcttcc
     2101 ccgcgtccgg gtgcgatatg atggcgaagg tcctccgccg ctggatctcc ttcaacagtt
     2161 gattcattat gctctgtccg ttttgcacca acccgcctgg cccagcggct ggctgctggc
     2221 cgttgcgcat tctaccccac gattccgccg ccaatcgcac gcctggcccg gctgccgccc
     2281 ggctacgtgg aatcgcacga ccgcagaggg cacactcggg agggtgcgaa tggaacggcg
     2341 cgcccgggca caaccgaaac gccggggcgg ccctcaatgc cgaagcccgc gagcgggcgc
     2401 gataggagct gagaggccgg ggtaccctgc cacgctggcg cgccgcgatg cacggtggcc
     2461 gtggggggcg ctccaggtcg ccgcgtgaag cggcggtttc gtccaacatc actgcataga
     2521 gaagattcta gcagaatcgc ctggtgatgt aaaggccgcg agcgcccgat ctggtacttc
     2581 tcggggcatg gcatgcaccc gggcgacccg acggtgctcg gcgccgaggc gggatttgtg
     2641 ttgtgcgggc cggcgccggg cagcgtgaag gtgatcacga ggtcacagcc cgcgaacgcg
     2701 gatctaagcg gggcacgggc atccatccac aacgcctgcc tcacggctct ggcccgagcc
     2761 tgattgacgt gatacacgcc aagggaccgt gcgccatcgg gtttgagaaa ccggtgtcga
     2821 acagtccggc aaccgagtgg gcgaagcgga tgtgggacag agccacgcgg gaaggcgtgg
     2881 agcttggcta agcggccaat atggcgaagg aggagttgac gatggatccg aaaggcaatc
     2941 ccaagcggta cggtggcatc gaaagcctcg tggtgaaggg tagctgcagg ctgtgggagt
     3001 gacgaacgtg cctgctcatg tgcagatcca gcccgcggaa ggagagccac gaatggaagc
     3061 tctttgtctt gcgctcgcgc tgtgctgggg tgcggcagcc ggtatgccgc cggaggggat
     3121 catggagcag gaggcggctc gcgtggcgcg cgctttcctt ggggatccaa cgatagcgct
     3181 gccgaagccg cgtatggtgg ggcccgggga tgacccgtcg ttctcctatg agctgccgga
     3241 tggccgtacg gtggcggtga accggagcac tgggcgggtc gagtggtact atgaccgtag
     3301 cgcggagctc gaccagcgac cggccgtcct ggtctcggag gcggatacgg agcgagagat
     3361 ccggcgcttc gcgcaagagc actatcccga tgtgctagag cggcacctgg tggttagagc
     3421 caactccagc ggtaagaatg tgtgggtcat atatatcgag cgcattcccg agaacggggc
     3481 gctgaaccgt atgggcagcc tgctcatcga ggcaaaccgc acaacgggtc gggtagccag
     3541 tgtcttggac aagtaccggc ccttaccccc ggaagcgagg cgcaagccga acgtctcacg
     3601 caaggctgct attgacacgg ccataggagc gacacagttc ctgcgtgtgt atggccgccc
     3661 cgaggtagaa ctggcgtgga ccaggcccat atttgcagac ggtccggcac tggtgtgggc
     3721 ggtgtcgttc aagggctacc tcggtcgcaa ccgcgcgaac agcgtcacgg tgtgggtgga
     3781 tgcgctcaag gggggcgtcg ttggcgagcc tgcctatggc gtggcttact tcgtggcgga
     3841 ggagaaccag tgtgtactcc atcaggactg ggggatgctc ccagcgattc ccattgtggt
     3901 gctgacgctg cgcccgcgcg ttgacgaggg caagctatgg atgcctgtgc gctttgccga
     3961 gttgttcgac ctgaaggtgg cgttggaggg cgatacggta tcgctttctg ggaaagacgg
     4021 cgagtgcgtt atcttggccc gcctgccggt gcgccgctgg gaggaagagg agtatctgcc
     4081 ggcggaggag atgctgaagg cggttgaaaa gcaggtgtgg gaggtcaagg cggacgtggc
     4141 gcatgcatcg ttgctagttg gcgtgcacag cgcgaagccg ctggagcgcg cgctggccct
     4201 gaagctagga aggcggccgt aggagcgcgt cttcgtaccc ggcagcgcca gcgccggcaa
     4261 gatccggggg caggcaaccg catgagatcc ccctgcgata ccggaaggat ccgctgggcc
     4321 ggacgcctgg cgagcaggcg cgaaggaatg ggggagccgg cgggcgaacc ccggagggag
     4381 aacgcaagcg tgccggacgc aacgcggttc aacgcgcggg tctgggcgca tgatagcggt
     4441 cctggagggt gatctgagat gaagaaggtg cgtatcggcg tgctgggcgt gggccggggc
     4501 gcggggttgg cgaggtcggc gcggctggct ccaagcgtgg agctcgcggc gctgtgcgac
     4561 atgagtgaag agcgcctggc aaaggcctcc gccacgatgg gcgtgcagac gacctacacc
     4621 tcctacgagg caatgctcga ttcggacgtg gatgcggtgc tggtggcgtc accgatgccg
     4681 ctgcacgtga agcacgcggt gcaggcgctg cgcgcgggca agcacgtgct ctcggaggtg
     4741 acggcggcga cgagcatcga agagtgctgg gagctgttcg aggcggtccg cgattccggg
     4801 ctgaaataca tgctggcgga gaactactgc tacatgcggc cttggagcat cgtgctcaac
     4861 atggt
//
LOCUS       DAOSCV010000244         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10977_length_4858_cov_3.927337, whole genome shotgun sequence.
ACCESSION   DAOSCV010000244 DAOSCV010000000
VERSION     DAOSCV010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10977_length_4858_cov_3.927337"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..680
                     /locus_tag="PLY56_07350"
     CDS             246..680
                     /locus_tag="PLY56_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21333.1"
                     /translation="MAAGSPRILVVDDEPGIVESLCEALKRTGYQVQTAFNGQQALDA
                     VEQACPDLIVMDVMMPGMDGLQALEILRGKAATRNTPIILLTALGTDMDIIAGVRAGA
                     TMYLTKPVENSRIVSLISAILGGRQALGCESAEDLRSAASKR"
     gene            688..954
                     /locus_tag="PLY56_07355"
     CDS             688..954
                     /locus_tag="PLY56_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21334.1"
                     /translation="MKDFAFYLGIGFTLLGLAYTAVVLFMRGATFGTERRPYVFNGIA
                     LILLGISLLARGLGVGNWLVYILWVVAAYLAIRASREILASRHR"
     gene            complement(951..1916)
                     /locus_tag="PLY56_07360"
     CDS             complement(951..1916)
                     /locus_tag="PLY56_07360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ21335.1"
                     /translation="MQQEMMGETPPAWQITRREPGRWEAAQQRDDGTTATITLICGDC
                     LEGMSGLQPGSIDVVVTSPPYNLGIRYSRYDDSIPREEYLEWIGRWAAAVKRVLAPQG
                     SLFLNVGSKPSDPWVPLQVALRVGDYLQLQNTIHWVKSIAIDPETMGRAGREATPLGI
                     GHFKPINSARFLNDCHEYIFHFTHAGDVPLDRLAIGVPYQDKSNVRRWRQAADDVRCR
                     GNTWFIPYDTIQNGKTQRPHPASFPIALPEWCYRLHGSNAASLCMDPFLGIGSSALAA
                     ARLGLSFIGFELDEGYFAEAVARLEARACHMESSQGRGTAGGSRR"
     gene            2332..2568
                     /locus_tag="PLY56_07365"
     CDS             2332..2568
                     /locus_tag="PLY56_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21336.1"
                     /translation="MAKREGEMTVREAGRKGGETTAERHGREFYEEIGHLGGEKGGEV
                     RKRQLGHEGYEELGHKGGQRVRELIEKGKEAESE"
     gene            complement(2668..3462)
                     /locus_tag="PLY56_07370"
     CDS             complement(2668..3462)
                     /locus_tag="PLY56_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ21337.1"
                     /translation="MKALILAAGYATRLYPLTKDRPKPLLPIGGRALIDYLVDQLETL
                     PDLDGIAVVTNHRFADHFRDWAHSRQARLPLEVLDDGTDTPENRLGAIGDAAWTIRIL
                     GLDEPLLIAAADNIFPFSFRDFAAFAAKKGTDCLSCYRLDDPERLKRTGIVAVSPEGR
                     VLRFEEKSPQPWSDLSVPPVYLYQRETLPLFEEYLAGGNNPDAPGHFVPWLLARRPVH
                     AFRFEGPVMDIGTPQSYAAACRAFESKAGTSRTAPPHAQETQEGRF"
     gene            complement(3459..4655)
                     /locus_tag="PLY56_07375"
     CDS             complement(3459..4655)
                     /locus_tag="PLY56_07375"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOJ21338.1"
                     /translation="MKTLSETQEAALTAAHSAGADMEAIRWVYSPLRICLLGAHVDHQ
                     GGDVLGTAIDRRILLAFSPSPDAQVALCSRDYEGRVCFSADAPPAELSPAWGRYAAGA
                     AVALNRRRPLARGIQGGIAGEAPVGGLSSSAAVGVAYLLALEHANALEVTPEENIELD
                     RIIENEYIGLKNGILDQSMILLSRKGALTAMNCRSGEYTHIPPGNDLPEFRLAVVHSG
                     IEQALVSTGYNRRVAECQEAARLLLEASGQPPRERPVLGDVSPELFAEYGHRLPADLA
                     KRARHFFTEQERTSEGRAAWAAGDLRRLGALIAASGESSITNYECGTPALIDLVRILN
                     GVPGVYGARFSGAGFRGACFALAAPDAGPAIEEALQREFNPRHPELAGRWHLDLCRPD
                     EGARVL"
BASE COUNT          808 a         1493 c         1697 g          860 t
ORIGIN      
        1 ggcgcgggtt tcctgaatcc cccggccctt tcctgccttc cggcccctcg cctggcctgg
       61 ggaaggggag cggattcgcg cggcaggcgc ggtttacagg caggcggata ggtgctataa
      121 tacgggcgcg ttgcaccttg ccgcccggtc attgccctcc ctcgcagtgg cccctggggc
      181 actgttcggg agtgactgcg ccagcaggtg ccagcaacac gtgctctgca gcaaggagga
      241 tgcccatggc agcgggttcg ccgaggatcc tggtagtgga tgatgagcca ggcatcgtgg
      301 agtccctctg cgaggcgctc aagcggacgg gctaccaggt acagacggcg ttcaacggcc
      361 aacaggcgtt ggatgcggtg gaacaggcgt gccccgatct gatcgtgatg gatgtgatga
      421 tgcctggcat ggatggcctg caggcgctgg agatacttcg cggcaaggcg gccacccgaa
      481 acaccccgat catcctgctg accgcgcttg ggacggatat ggatatcatc gcgggggtca
      541 gagcgggcgc cacgatgtac ctcaccaagc cggtggagaa ctccaggatt gtctcgctca
      601 tttccgccat tctggggggg cggcaggcgc ttggatgcga gagcgcggag gatctccgtt
      661 cggccgcttc aaagagatag gcactctgtg aaggatttcg ctttttacct ggggatcggt
      721 ttcaccctcc tcggcctggc ctatacggcc gtggtcctct tcatgcgcgg cgccactttt
      781 ggcacggagc gccggcctta tgtcttcaac ggcatcgcgc tcatccttct tgggatctcc
      841 ctgctggcac gcggtctggg agtcggcaac tggcttgtct acatcctctg ggtggtggct
      901 gcttacctcg ccatccgcgc cagccgcgag attctggcct cgcgccaccg ctagcggcgc
      961 gatccccctg cggtcccccg gccctggctc gattccatgt ggcacgcgcg tgcctctaac
     1021 cgcgctactg cctccgcgaa atagccctca tccagctcga agccgatgaa ggagaggccg
     1081 agccgcgcgg cggcaagcgc ggagctgcca atgcccagga agggatccat gcatagagac
     1141 gcggcgttgc tgccatgcaa ccggtagcac cattcgggga gggcgatggg gaaagaggcc
     1201 ggatgggggc gttgcgtctt gccgttctgg atggtgtcgt acgggatgaa ccaggtgttc
     1261 ccacggcagc gtacgtcgtc cgccgcctgc cgccagcggc gaacgttcga cttatcctga
     1321 tacggcacgc cgatggcaag tcggtcgagc ggcacgtcgc cggcgtgcgt gaagtgaaag
     1381 atatactcat ggcagtcgtt tagaaaccgc gctgaattga tcggcttgaa gtggccgatc
     1441 cccaatggcg tggcctcccg cccggcacgc cccatcgtct ccgggtcgat ggcaatcgac
     1501 ttcacccagt ggatggtgtt ctgcagttgc agataatccc ccacgcgtag agcgacctga
     1561 agaggtaccc agggatcgct cggcttgctc ccgacgttta gaaagagcga gccctgcggg
     1621 gccaaaactc gcttcacggc cgctgcccag cggccgatcc actccaggta ctcctcccgc
     1681 ggaatggaat cgtcataccg ggaatagcgg atacccaggt tgtagggggg cgaggtcacc
     1741 actacgtcga tgctgcccgg ttggagcccg gacattcctt cgagacagtc gccgcagatc
     1801 aacgtgatcg tggcggttgt tccatcgtcg cgctgttggg ctgcctccca tcggccgggc
     1861 tcccggcgag tgatttgcca ggcgggtggt gtctctccca tcatctcttg ctgcatcgtt
     1921 ggtctttgtt tctgaagccg gctccgaaaa ccgcgagaga gaccagcgag ggcggttctg
     1981 gctcggcggg tgggccaact gaagggtgat tcgcggaaca cgtcattggg gcccgcggtt
     2041 gttacttgtc gcgtggcgtg ctgatgggtt cttgttcgcc atgagcgacc cgcctccctt
     2101 ttcttcccga gtagccaggg cgggggtgca tgactcccgg ggggccttcg cgtcactgcc
     2161 agcggttcat ttcgtcatcc ggagaccatc acaggtatta tctgtgttca tctgtgccat
     2221 ctgcggatac tccccataga ggacgtttgg ggcacaaagg ggccgggtac gacggggacg
     2281 gctgggggcg gcggatgcca tgccggctcc gggatgggga ggtgaagcgc gatggcaaaa
     2341 cgcgaaggcg agatgacggt tcgcgaggcc ggccgcaagg gcggcgagac gaccgcggag
     2401 cgccatggcc gcgagttcta cgaggagatt ggccacttgg gtggcgagaa gggcggcgag
     2461 gtgcgcaagc ggcagcttgg gcacgagggc tacgaggagc ttggtcacaa aggcggccag
     2521 cgcgtacgcg agttgatcga gaagggcaag gaagctgaga gcgagtaacg ggcctggagg
     2581 agccgggcga gcaggtggcg cgggcgtcag ggcccgcgtc gcctgctttg cgcgggcgta
     2641 aacccccgcg cgtaatcctc ggttccctca gaagcggccc tcttgggtct cctgcgcgtg
     2701 gggcggggcg gtgcggctgg ttccggcttt tgattcgaag gcacggcagg cggccgcgta
     2761 gctttgcggg gtgccgatat ccatcacagg gccctcgaag cggaaggcat gcaccgggcg
     2821 ccgggcgaga agccacggta cgaaatggcc gggcgcgtcc gggttgttcc cgcccgccag
     2881 gtactcctca aaaagcggca acgtctcgcg ctggtacagg tagaccggag gcaccgatag
     2941 gtcgctccag ggctggggcg acttctcctc gaagcggagg acgcggccct cgggggagac
     3001 agccacgatt cccgtgcgtt tcaggcgctc cggatcgtcc agccggtagc aggaaaggca
     3061 gtccgtgcct ttcttggcgg caaaggcagc aaaatcccgg aaggagaagg ggaagatgtt
     3121 gtcggccgcg gcgatcagga gcggctcatc gagaccgagg atccggatcg tccaagcagc
     3181 gtcgccgatg gcgcccagtc ggttctccgg ggtatcggtg ccgtcgtcga gcacttccag
     3241 gggcaggcgc gcctgcctgc tgtgggccca atcccgaaag tgatcggcaa agcggtggtt
     3301 ggtcaccacg gcgatgccat ccagatccgg cagagtctcc agttgatcca ccaggtagtc
     3361 gatcagggcg cgcccgccga tggggaggag cggcttgggt cggtctttgg tcaggggata
     3421 gaggcgggtg gcataacccg cggcgaggat gagcgctttc atagaactcg ggccccttca
     3481 tctgggcggc acaggtcgag gtgccaccgt ccggccagtt cgggatgacg cggattgaac
     3541 tcgcgctgta gtgcttcctc gatggccggc cccgcatccg gagccgccag cgcgaagcag
     3601 gcgcctcgga agcccgcccc gctgaagcgc gcgccataga cgccgggaac gccgttcagg
     3661 atgcgcacga gatcgatgag cgccggcgtg ccacactcgt agttggtgat ggaactctcc
     3721 ccggaagcgg cgatgagcgc gccgaggcgg cgcaggtcac ccgcggccca ggccgcgcgg
     3781 ccctcgctcg tccgctcctg ttccgtgaag aaatgccggg cacgcttcgc tagatcggcc
     3841 ggcagccggt gaccgtactc cgcgaacagc tccggggaaa cgtcgcccag cacggggcgc
     3901 tcgcgtgggg gctggccgga cgcctccaac agcaaccgtg ctgcctcctg acactcggcg
     3961 acccggcggt tgtagccggt gctcaccaac gcctgctcga tccccgagtg gaccacagcc
     4021 agccggaatt ccggcagatc gttgcccgga ggaatgtgag tgtactcccc gctcctgcag
     4081 ttcatcgcgg tcagcgcgcc cttgcggctg aggaggatca tcgattgatc caggatgccg
     4141 ttcttcaggc cgatgtactc gttttcgatg atccggtcta gctcgatatt ctcctcgggc
     4201 gtgacttcca gggcgttcgc gtgttccaga gcgaggaggt aggcgacacc cacggcagcc
     4261 gaggagctga ggccgcccac gggagcctcg ccggcgatcc cgccctggat cccgcgcgcc
     4321 agcggtcgcc ggcgattcag ggcaaccgcc gcgcccgcag cgtagcgtcc ccaggccggc
     4381 gagagctccg ctggcggagc atccgccgag aagcagacgc ggccttcgta atcgcgactg
     4441 cagagggcca cctgcgcgtc tggcgaggga ctgaacgcga gcagaatccg ccggtcaatg
     4501 gcggtgccga gcacatcgcc gccctggtgg tccacgtgcg cgccaagcaa gcagatccgc
     4561 aggggcgaat agacccagcg gatcgcttcc atgtcggcgc ccgcggaatg ggcggccgtc
     4621 aaggcagctt cttgtgtctc cgagagcgtt ttcatgttca tcctcggggg cgaggggggc
     4681 cccgcgttga tacggcctgg tgtcggggcc tcgagtcttc tctgctccac cggcagggga
     4741 atgcgcgtga ctcactgacc gatttcgcgc gcgcgctcct gagtcctgcc catgctgaat
     4801 gggattgggc gtggcaaaga actggtcagg ggagggggat gcgtggtggc cggcggag
//
LOCUS       DAOSCV010000245         4855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10988_length_4855_cov_4.847917, whole genome shotgun sequence.
ACCESSION   DAOSCV010000245 DAOSCV010000000
VERSION     DAOSCV010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10988_length_4855_cov_4.847917"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="PLY56_07380"
     CDS             complement(<1..120)
                     /locus_tag="PLY56_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21339.1"
                     /translation="MKRRHTGFTLIELLVVIAIIAILAAILFPVFARARENARK"
     gene            complement(580..1251)
                     /locus_tag="PLY56_07385"
     CDS             complement(580..1251)
                     /locus_tag="PLY56_07385"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21340.1"
                     /translation="MKRRHTGFTLIELLVVIAIIAILAAILFPVFARARENARKSSCQ
                     SNLKQLGMAVQQYAQDYDETYPGYCIDCSTSVTYVLPNGATQTGQYVLWPVLIYPYVK
                     NTQVYNCPSSTTKWTGNYASAMCYGLNARSFGTSGLAMASVNYPSDLMYFADANTAAS
                     NSYHFSSGDAADTDSDGIKDYKVDKRHSEGANVCYADGHVKWIKVNAVPEPTASSRFW
                     YYNAP"
     gene            1430..2233
                     /locus_tag="PLY56_07390"
     CDS             1430..2233
                     /locus_tag="PLY56_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ21341.1"
                     /translation="MLNAQEIKRVAREEYGADLVGIASIDRFANLPAEASPVSIQPDA
                     RAVIVLGFSIPRGGLRGVEAGTAWSTFNLSLPTGTIVTECTYLVCRHLESEGWEATPL
                     YPQSADLRGQGVRVHPDKPEPNVILDLVYAAHAAGLGEIGRGKFFLTPEFGVRQILTA
                     ILTDAPLEADAPFRGSLCDDCGACAEACPARALDRSRLNEAALCEGTARWYPLHLESC
                     RVCKTCVLPMPYSTGSEPLRVAAACGRACVAHLEDSGKLTRKFASRFRE"
     gene            2246..3313
                     /locus_tag="PLY56_07395"
     CDS             2246..3313
                     /locus_tag="PLY56_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21342.1"
                     /translation="MLTAERLKEFALNEVRVDKIGIANIERFAQAPPDMHPLNIMPNA
                     RSVVVFIKRIVRGTYRGIDEGTHWPSYSIFGYAHLNRMLGSSAYKIARFIERFGYEAT
                     GLSGAAASREIGVRGPRPAPGKPAREVVLHHRIAATLAGLGEIGWSKVFLTEEFGPRQ
                     RIGVVLTEAELEPDPIRTGHLCDRCKRCAKECPAGAISTERSVGIEVEGHRIEWGDLD
                     LGKCKLTHFGLNKRSSPFFAKRFPGLSLPVSEQEVTWREAWDLGYALFPTMPAFQSLA
                     AHPIAICGARGCIQGCMKHLEKRGKVKNRFRTRPVFSEAEPWCLPETPAHTFAEHHGF
                     IYDPEVDEVEDVESQAGSSWY"
     gene            3822..4070
                     /locus_tag="PLY56_07400"
     CDS             3822..4070
                     /locus_tag="PLY56_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HOJ21343.1"
                     /translation="MKVTYDEQVDAVYCYLSDREAARTIQVSRHVLLDLDSDENLRGI
                     SILDASRIFPAAPYAGGLLVDEDMEPGDWPLLMPLGHA"
     gene            complement(4180..4749)
                     /locus_tag="PLY56_07405"
     CDS             complement(4180..4749)
                     /locus_tag="PLY56_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOJ21344.1"
                     /translation="MADRTSNLDVLSRKRIVIINGEIREEMVENIVKELFRLEAANPT
                     EDISVYINSPGGSVIAGLAIHDVMKMVRCDISTVCYGQASSMAALLLMSGARGKRYIS
                     PNARVLIHQPSGGAFGTATEVETTALEITRIKKRLLEMVSFYTGRPIEQVAIDMERDF
                     YMDADAAVRYGVVDQIRYPGQGGTPSADS"
BASE COUNT          877 a         1454 c         1615 g          909 t
ORIGIN      
        1 cttgcgcgcg ttctcccgcg cccgcgcgaa caccggaaag aggatcgcgg ccaggatggc
       61 gatgatggcg atcacaacga gcaactcgat cagggtgaag ccagtgtgtc ttcgtttcat
      121 gccagaagtc ctttctcgaa taggctcccg attggcggcg gtaccattcg cgcaagggca
      181 ttataccaca acacggcccc gatgtgcaac cctgtctcca ggagtcgcat cgggttccat
      241 cctcctgcat caggaggttc ttgggggagg gagccaccgg aggccatcct gaggctgctt
      301 ttcctcgcgc gacgggagca caccgccctc tcttggtgcc acgggatctt gaagggcccg
      361 gggctctagt cagcgcggat gggggggatg ggcacggtgg gcggcgaacc atgagatgct
      421 gttgcagacg cgcgatgggg tcaaccacct tacggggctc actaccggga actttggggg
      481 cctggaggga gaggtgtgcc ggaggcagcc gccccgtacg aagcggctgc cctggccact
      541 tgtttattgc gcgatggaga gtcggtggct accagccccc tagggcgcgt tgtagtacca
      601 gaagcggctg gaagcggtgg gctcgggcac cgcgttgacc ttgatccatt tcacgtggcc
      661 atccgcgtag cagacgttgg cgccctccga atgccgcttg tcgaccttgt agtccttgat
      721 cccgtcggag tcggtatccg cggcgtcgcc ggagctgaag tgatacgagt ttgatgccgc
      781 cgtgttcgca tcggcgaagt acatcagatc cgaggggtag ttgaccgagg ccatcgccag
      841 gcccgaggtg ccgaaggagc gcgcgttcag gccgtagcac attgcgctcg cgtagttgcc
      901 cgtccatttg gtggtgctgg aggggcagtt gtaaacctgt gtgttcttca cgtaggggta
      961 gatgagcact ggccagagga catactggcc ggtctgggtg gcgccattcg gcagcacata
     1021 ggtgacgctc gtcgagcagt caatgcagta cccgggatag gtctcatcgt agtcctgagc
     1081 atactgttgc acggccatgc ccagctgttt caggttcgat tggcaggagc tcttgcgcgc
     1141 gttctcccgc gcccgcgcga acaccggaaa gaggatcgcg gccaggatgg cgatgatggc
     1201 gatcacaacg agcaactcga tcagggtgaa gccagtgtgt cttcgtttca tgccagaagt
     1261 cctttctcga ataggctccc gattggcggc ggtaccattc gcgcaagggc attataccac
     1321 aacacggccc cgatgtgcaa ccttatctct aggagccgct tccggtttcc ggccgccctg
     1381 ggcaaggagg ccacgggaga tctgagtctc gaaacacaag gaggaacgca tgttgaatgc
     1441 acaggagatc aagagggtgg cgagagagga gtatggcgcc gatctggtcg gcatcgcctc
     1501 catcgaccgt tttgccaacc ttccggcaga ggccagcccg gtaagcatcc agccggatgc
     1561 cagggcggtc atcgtgctcg ggttcagcat ccctcgtggt ggcctgcgcg gcgtcgaggc
     1621 ggggacggcc tggagtacct tcaatctgtc gctccccacg ggaacgattg tgacggagtg
     1681 tacctacctg gtctgccggc acctcgagtc cgaggggtgg gaggccacgc cgctctaccc
     1741 acagagcgcc gacctgcgcg gccagggggt gcgcgtccac ccagacaagc cggagccgaa
     1801 cgtgatcctg gatctggtct atgccgcaca cgcggcgggc ctgggcgaga taggacgcgg
     1861 gaagttcttc ctgacgccgg agttcggggt gcgccagatc ctgaccgcta tcctcacaga
     1921 tgctcccctc gaagcggatg cgcctttccg tggatcgctg tgcgatgact gtggcgcttg
     1981 cgccgaggcg tgcccggcgc gtgccctcga ccgctcgcgc ctcaacgagg cagcgctgtg
     2041 cgagggcacg gcgcgatggt atcctctcca cctggagagc tgccgggtgt gcaagacgtg
     2101 tgttctcccg atgccgtatt ccaccgggtc cgagccgctg cgcgtggccg cggcgtgcgg
     2161 acgtgcctgc gtcgcgcacc tggaggatag tgggaagttg acgcgcaaat tcgcgtcgcg
     2221 gttccgggaa tagggaaggt gcgccatgct gacagccgag agactgaagg agttcgcgct
     2281 caacgaggtg cgcgtcgata agatcggcat cgccaacatc gagcgttttg cgcaggcgcc
     2341 tcccgatatg catccactga atatcatgcc gaacgcgcgt tccgtggtcg ttttcatcaa
     2401 gcggatcgtg cgcggcacct accggggaat tgacgagggc acgcactggc cctcctattc
     2461 gatcttcggc tatgcccatc tcaaccggat gctaggctcc tcggcctaca agattgcccg
     2521 gttcatcgag cgttttggct atgaggccac gggcctcagt ggcgcggctg ccagccgcga
     2581 gatcggcgtg cgcggcccac gtccggcgcc tggcaagcct gcgcgcgagg tggtgctgca
     2641 ccatcgaatc gcggcgaccc tggccgggct cggggagatt ggctggtcga aggtcttcct
     2701 caccgaggag ttcgggcccc gccagcgcat cggggtggtc ctcactgaag ccgagctgga
     2761 gccggatccg atccgcacag gccatctgtg tgaccggtgc aagcggtgcg cgaaggagtg
     2821 cccggcgggc gccatttcaa ccgagcgctc ggtggggatc gaggtggaag gccaccgcat
     2881 cgaatggggg gatctggacc tggggaagtg caagctgacg cactttggcc tgaacaagcg
     2941 gtcgagcccc ttcttcgcga agcgtttccc ggggctttcg cttccggtga gcgagcagga
     3001 agtgacctgg cgagaggcct gggatctcgg ctatgcgctc ttcccgacga tgccggcgtt
     3061 tcaatcgctc gccgcgcacc caatcgccat ctgtggggcg cgcgggtgca tccagggttg
     3121 catgaagcac ctggagaagc ggggaaaagt gaagaaccgc ttccgcaccc gcccggtctt
     3181 cagcgaggcg gagccatggt gcctccccga gacgccggcg cacaccttcg cggaacacca
     3241 cgggttcatc tatgaccccg aggtggacga agtggaggat gtggagagtc aggccggatc
     3301 gagctggtac tgacgccacg aagcgctgtt tggaggcgca tcacggggcc gggcgtgggc
     3361 gccccgcccg cgtccggccc caaaacccgt cgcgttcgta cggagcgaag gcagggcgaa
     3421 ccccgaaggt tcgccggcgg gggtggcgcc ttctcgcccc tgaaaggggc gtggaacacg
     3481 gcccaggatg tctgcaccca gggtgacgca ccggaaaatg tgacaacgac cctaaagggg
     3541 tcggacaaac cgctactgcg taatgtcgca ccctttcagg gtgctagctg ggattccgac
     3601 ataaggagag ggccgcctgc gcgcgcggag ctcgaagctc cgcgctgctt tcggcctgcc
     3661 atccgcggaa tcgctgccgg cgggggcagg gaagctggcc gtcactctct ccataagccc
     3721 tcacatgaat cccggtagaa ctgaccttga cacgcggttg gagactggca tataatctga
     3781 cccgtatgtc ctgagtgggg aatagggaat tggagacgtg gatgaaggtg acttacgacg
     3841 agcaggtgga tgcggtatac tgctatctca gcgatcgtga agctgcccgc acgattcagg
     3901 tatcgcgtca tgtcctcctc gatctggaca gtgacgagaa cctgcgcggg atcagcatcc
     3961 tcgatgcgag ccgcatcttc ccagcggctc cgtacgcggg gggtctgctc gtggatgagg
     4021 atatggagcc gggcgactgg ccgttgctca tgccactagg ccacgcctaa gatctgaaac
     4081 cgcggagtgc agtttcaaat cggcctccct taccccaaat tgctggggta agggaggccg
     4141 atttcttgtt gaaggatccc tggctgccgg tacgcctggt caagaatccg cggaaggcgt
     4201 gccgccctgg cctgggtagc ggatctggtc cacaacgccg taacgcaccg ctgcatcggc
     4261 gtccatatag aagtcgcgct ccatatcgat ggccacctgc tcaatcggcc ggccggtata
     4321 gaaggaaacc atctccagga gccgcttctt gatgcgagtg atctcgagag cggtggtctc
     4381 cacttccgtc gcggttccaa aggcgccgcc actcggttgg tggatcagca cgcgagcgtt
     4441 tgggctgatg tatcgcttgc cacgcgcgcc ggacatgagc agcagtgccg ccatcgagga
     4501 ggcctgcccg tagcagactg tggagatatc gcaccgcacc atcttcatca cgtcgtggat
     4561 ggctagcccc gcgatgacgc tcccgcccgg cgagttgatg tagacgctga tatcctccgt
     4621 ggggttggct gcttccaggc ggaacagctc cttgacgatg ttctccacca tctcctcgcg
     4681 gatttcgcca ttgatgatga cgattctctt ccgcgatagg acatcgaggt tgctcgtacg
     4741 atctgccata aaccccttcc ctttgccgta cggcgcggat gcatgagagg atcctcgacg
     4801 tatggcaacg ccggcagcgc cggaatagtc gtgaagccag caagagggca cgcgc
//
LOCUS       DAOSCV010000246         4853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_10995_length_4853_cov_3.594414, whole genome shotgun sequence.
ACCESSION   DAOSCV010000246 DAOSCV010000000
VERSION     DAOSCV010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10995_length_4853_cov_3.594414"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1313)
                     /locus_tag="PLY56_07410"
     CDS             complement(207..1313)
                     /locus_tag="PLY56_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOJ21345.1"
                     /translation="MFDTIAVIGAGNGGKASAADLALQGKRVRLFELPEYAGNLEAVA
                     QSRRLIATGAVAGEAELERATTELEAAISGAELILVCTQAPAHDRVARILAPLVRPEQ
                     VVVLSPGSTGGTLHFANVFRQMGVERLPILVELSTLPYGCRASGESVRVFVKVGRVKY
                     GVLPGRETERIGPALEALYPGFVRSESVLEAGLNNGNPVIHPAVTLLNAARIEKEGTA
                     MYFYRDGVSPAVARAIEALDGERMALLRALGYPAQPEPLTCVQQGYATASGYLECYSQ
                     GPGFIDFRSPDTLDHRYLHEDIGMGLLFYTSLGRLLGVPTSLSDAFVTMGSALSGIDY
                     RARNARSLESLGLSGITVEALKEYLQSGILPPGA"
     gene            1587..2069
                     /locus_tag="PLY56_07415"
     CDS             1587..2069
                     /locus_tag="PLY56_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOJ21346.1"
                     /translation="MQHLWAPWRMAYVGVEQPQGCIFCVKPAEDRDAENYLVHRGKHA
                     FVMLNAFPYSSGHLLIAPYRHTADFTGLSEEELLELMQLAQQGITVLQRVYRPEGCNL
                     GMNLGRVAGAGIAEHLHLHVVPRWGGDTNFMTVVADTRVLPEALADSYRRLREGWEAL
                     "
     gene            2066..2743
                     /locus_tag="PLY56_07420"
     CDS             2066..2743
                     /locus_tag="PLY56_07420"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ21347.1"
                     /translation="MTEIAALEALKAECLQCQACPLAKTRTKVVFDDGNPNAPLVIIG
                     EGPGENEDRTGVPFVGRAGKLLDRVLLENALSRRWVYICNTVKCRACDISEGRARNRA
                     PLPAEMAACRPYLERQLALIRPVVILCLGGPAASTIIHKDFRMTQERGRWFTDSPFAP
                     WVMASWHPAYVLRQGGEAYARTRAELVADIDQCRKKVIEIRQQGGLLARQVAQAEPVE
                     ESEPGLF"
     gene            2906..3358
                     /locus_tag="PLY56_07425"
     CDS             2906..3358
                     /locus_tag="PLY56_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21348.1"
                     /translation="MSRRRQLAAALVAALALGTAGARAAKIDLGSIGDAVKIMGIGFA
                     VKTFGGQINQAINTVLMQNNIERHGATKVVPILSVGQGAYIGAAQVTGPENEVAQVQA
                     VVQGEVTASGGQVRFNALVPVDSINPFQSLPKPKFKGVGITAIIDFNV"
     gene            3375..4085
                     /locus_tag="PLY56_07430"
     CDS             3375..4085
                     /locus_tag="PLY56_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21349.1"
                     /translation="MNRRKAIALPLGVIGLALLLVAALPSQTRRAAAQEDWFTSEPEE
                     IQVTSRAQELGGLPVGEVLLGESVVTRIRSAAGGKTAPQRAETAAARLSGVLRAGSPA
                     PEEFRVAQMNGQYVVAVRDELIVTADTYHAQVNGTTPVKLATQWRDNLVQAYRRELDR
                     PIGKICPIVSVGKGTRLGLAHITGPRSQVHKAQFVGQLETTFQKSVRIRIFVPIKAQD
                     GVDRVPQVNVNAYADLKM"
     gene            4287..>4853
                     /locus_tag="PLY56_07435"
     CDS             4287..>4853
                     /locus_tag="PLY56_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HOJ21350.1"
                     /translation="MIAAAIDVGTNSVLLTVGERLADGEVRILEERSEITRLGQGVDH
                     ARTLRAEAMERTATAVAGFASRARELGAVHLRVVGTSAARDARNATEFACLLRQRAGV
                     ELEILSGEEEARLSYQAVRADPSLGLPAGDLIVVDIGGGSVECIWGDEAGIRFHASID
                     CGAVRVTERFFASDPPTLAERDRAIHWID"
BASE COUNT          814 a         1591 c         1632 g          816 t
ORIGIN      
        1 agccccgaag ggctttgccg gaaagcagcg cggagcttcg agctccgcgc gtgcgtgcgg
       61 ccttctcctt atctcggaat cccagccgag ctcgccaggg cggggcggtg ccgcaggcaa
      121 tgtgattctc gatgaatgtt ttgtaggggt ggccccttgg tattcgccct gtttcctcgc
      181 gggcggctga cggtcgattg ggagttctac gctccagggg gcaggatgcc gctctgcaaa
      241 tactccttca gagcttcgac cgttatcccg ctcagaccga gggactcgag cgagcgcgcg
      301 ttcctcgcgc ggtagtcgat gccggagagc gcggatccca tcgtcacgaa agcgtcgctc
      361 agagacgttg gcacgccgag gagccggccg agcgaggtgt agaagagcag gcccatgcca
      421 atatcctcgt gcagatagcg atggtcgagc gtgtcggggc tgcggaaatc gatgaagccg
      481 ggcccctgcg agtagcactc cagatagccg gaagcggtgg catagccctg ctgcacgcag
      541 gtcaacggtt ccggctgcgc tgggtatccg agagcgcgca ggagagccat gcgctcgccg
      601 tccagcgcct cgatggctcg tgccaccgcg ggcgagacgc cgtcgcgata gaagtacatg
      661 gctgtgccct ccttctcgat gcgcgccgca ttgaggaggg tcaccgccgg gtggatcacc
      721 ggattgccgt tgttcaggcc ggcctccaac acgctctcgg agcgcacgaa ccccggatag
      781 agcgcctcaa gcgcgggccc gatccgctcc gtctcgcgcc ccgggagcac gccatacttc
      841 acccggccca ccttcacgaa aacgcgcacg ctttctcctg aggcgcggca gccgtaagga
      901 agcgtagaga gctccaccag gattggcagc cgctccacgc ccatctggcg aaagacgttc
      961 gcgaagtgga gcgtgccgcc ggtggagccg gggctgagga ccaccacctg ctccgggcgg
     1021 accaggggcg ccagaatccg tgcgacgcga tcatgcgccg gtgcctgcgt gcagacgagg
     1081 atcagctctg cgcccgagat cgctgcctcc agctccgtgg tggcgcgctc cagctctgcc
     1141 tcgcccgcga ccgcccccgt ggcgatcaac cgccgcgatt gcgcgacggc ctccaggttc
     1201 ccggcgtact cgggcagctc gaaaagccgg acgcgcttcc cctgcaaggc caggtcggca
     1261 gcggatgcct tgccgccgtt gcctgcaccg atcaccgcga tcgtatcgaa cacttcgctc
     1321 tcccgtcttt atatcactcg cgtgagggcc agaccgcttt gccccccacg agcattccag
     1381 gcgcttgcgc ccgcgtgtga taccaccgtg cccttggggc acccttcgcc tcggaagagg
     1441 ttcgccagcc ggggcgctct tcctgtccgg atggcaccca ccgcgtctcc ccgcgccgga
     1501 gtggcgggag gatcgctgcg agcgtcgaac cttacacttg agcagcgtgc cagcgtcttc
     1561 gcatgctggg agggagagtt cgagtcatgc agcatctttg ggctccgtgg cgcatggcct
     1621 acgtgggggt tgagcaaccg caggggtgca tcttctgcgt gaagcccgcg gaggatcgcg
     1681 acgcggagaa ttacctcgtg catcggggaa agcacgcctt tgtcatgctg aatgcgttcc
     1741 cctatagtag cggccacctg ttgattgccc cgtaccggca taccgccgat ttcaccggcc
     1801 tgagcgaaga ggagctgttg gagttgatgc agctcgctca gcaaggcatc accgttcttc
     1861 agcgggtcta ccgcccggag gggtgcaacc tggggatgaa cctcgggcgc gtggctgggg
     1921 cgggcatcgc cgagcacctc cacctgcacg tcgtcccccg ttggggaggc gacaccaact
     1981 tcatgaccgt ggttgccgat acccgggtgc tgcccgaggc gctcgccgac tcttaccgcc
     2041 gtcttcgcga ggggtgggag gcgctgtgac tgagatagcc gctctcgagg ctctgaaggc
     2101 ggagtgcctg cagtgccagg cctgccctct ggcgaagacg cgaacgaagg ttgttttcga
     2161 cgatggcaac ccgaacgcgc cgctggtgat catcggggaa ggccccggag agaatgagga
     2221 ccggacgggg gttcccttcg tgggtagggc tgggaagctg ctggatcgcg tgctcctgga
     2281 gaacgcgctg agccgcaggt gggtctacat ctgcaacacg gtgaagtgcc gcgcctgcga
     2341 tatctccgag gggcgcgcga ggaaccgggc accgctgcca gcggagatgg cggcctgccg
     2401 cccctacctg gagcgccagc tcgcgttgat ccgccccgtg gtcatcctct gcctgggcgg
     2461 gccggcggca agcaccatca ttcataagga tttccgcatg acccaggagc gcggtcgctg
     2521 gttcaccgat tcccccttcg ccccgtgggt gatggcctcg tggcatcctg cctacgtcct
     2581 tcgccagggg ggcgaagcct atgcgcgcac ccgcgccgag ctcgtggctg atatcgacca
     2641 gtgccgcaag aaggtgatcg aaattcgcca gcagggaggc ttgctcgccc ggcaagtggc
     2701 ccaggctgag ccggttgagg agagcgagcc cggtctcttc tgaccaggcg caatcggacc
     2761 gccgctcgcg cgtctccgaa ccgaatgcct gctagcgtcg tctcaagcca cgaaccaccg
     2821 acgaccggcc tctctcgcgg ggacgggcaa tgcctcccat actcgcgtga gaggacgctg
     2881 ccggacagtg aaaggaggag acaggatgtc acggagaagg caactcgcag cggccctggt
     2941 cgccgcgctg gccctcggaa ccgccggtgc ccgcgcggcc aagatcgacc tggggagcat
     3001 cggggatgcc gtgaagatca tggggatcgg cttcgccgtc aagaccttcg gcggccagat
     3061 caaccaggcg atcaacacgg tcctgatgca gaacaacatc gagcgccacg gcgccaccaa
     3121 ggtggtacca atcctctcgg tgggccaggg cgcttacatc ggcgccgcac aggtcaccgg
     3181 gcctgagaac gaggttgcgc aggtccaggc cgtcgttcag ggcgaggtca cggctagcgg
     3241 cggtcaggtg cgcttcaacg cgctcgtgcc cgtcgactcc atcaacccgt tccaatcgct
     3301 gcccaagccc aagttcaagg gtgtcgggat cacagcgatc atcgatttca acgtgtaatg
     3361 agaggatgcc ggaggtgaac agacgcaaag caatcgcgct gccactcgga gtgatcggcc
     3421 tggccttgtt gctggtcgcc gctctgccga gccagacgcg ccgcgccgca gctcaggaag
     3481 actggttcac ttcggagcct gaggagatac aggtgacatc cagagcgcag gagctcggcg
     3541 gactgccggt gggcgaagtg ctcctcggag agagcgttgt cacccgcatt cgctcggccg
     3601 ccggcggcaa gacggccccg cagcgagccg agaccgcggc tgcccgcctc tcaggcgtac
     3661 tgcgcgcggg aagcccggcg ccggaggagt tccgtgtcgc ccagatgaat ggccaatacg
     3721 tggtggcggt gcgcgacgaa ctcatcgtca cggccgatac ctaccacgcg caggtgaatg
     3781 gcactacccc ggtgaaactg gccacccagt ggcgcgacaa cctggtccag gcctatcgcc
     3841 gcgagctgga tcgccccatc ggcaagatct gccccatcgt cagcgttggc aagggcaccc
     3901 gcctcggcct cgcgcacatc accgggccgc gctcgcaggt ccataaggcc cagttcgtcg
     3961 gccagctgga gacgacgttc cagaagagcg tgcgcatccg catctttgtg ccgatcaagg
     4021 cccaggatgg cgtcgatcgt gtgccgcagg tgaacgtcaa cgcctacgcc gatctgaaga
     4081 tgtagccctc gcttcgacac gccggaggag ggcgcctcgg tgaagccacc ctcctccctt
     4141 tccccctcct cacgcttgaa accgcccctg tgcgtgccct atggtgccgt ctggtaggtg
     4201 acccaggccg gggcaggtag ggaagagtgg cggcgccccc tctgaaccgg cgctgcccga
     4261 cggccaatct ctcgcgggag aatgtggtga tcgctgctgc aatcgatgtc ggtacgaact
     4321 cggtgctcct cacggtcggg gagcggttgg cagatggaga ggtgcgcatt ctggaggagc
     4381 ggagcgagat cactcgcctg gggcagggcg tcgatcatgc gcgcacactc cgcgcggaag
     4441 cgatggagcg caccgcgacc gcggtggccg gctttgccag ccgcgcgcgt gagctgggcg
     4501 ccgtgcatct gcgcgttgtg ggcaccagtg ccgcgcgcga tgcccgcaat gccacggagt
     4561 tcgcctgtct cctgcgccag cgagccgggg tcgaattgga gattctctcc ggcgaggagg
     4621 aagctcggct ctcctaccag gccgtgcgtg ccgacccctc cctgggcctc cccgcgggcg
     4681 atctgatcgt ggtcgatatc ggcggcggga gtgtggagtg tatctggggc gatgaggcgg
     4741 gcattcgctt tcacgccagc atcgattgcg gggctgtgcg cgtcaccgag cggtttttcg
     4801 cctcggatcc ccccaccctg gcggagagag accgcgcgat ccactggatt gat
//
LOCUS       DAOSCV010000247         4848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11010_length_4848_cov_3.280409, whole genome shotgun sequence.
ACCESSION   DAOSCV010000247 DAOSCV010000000
VERSION     DAOSCV010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11010_length_4848_cov_3.280409"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            999..1391
                     /locus_tag="PLY56_07440"
     CDS             999..1391
                     /locus_tag="PLY56_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21351.1"
                     /translation="MAKILVVDDEPNIVKLTSFHLRRHGHEVVSADNGAAALVTANQE
                     RPDLIILDVMMPVMDGFETLRSLRAAEETREIPIIMLTCRNLDHDIAHGLMEGADLYI
                     TKPFEMDKLMLAVRRLLAVAGDAAGTTD"
     gene            1687..2382
                     /locus_tag="PLY56_07445"
     CDS             1687..2382
                     /locus_tag="PLY56_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21352.1"
                     /translation="MAGPGDPGMDAPGAAEPAGGGAASEEAISFTQMPFVTRPKRVMV
                     RQPGGKLVEHVEFRYHAKGDPEALLAFLNPSPSAAPDEGSVWFVRMPHAATAGKKTPG
                     EWENLFCTYVVPDNESIERSRRALQARLTQLRNQVYRQKIYTVDLLAGDRLPQTAQVN
                     AMDTAASYVPDPSARLSLYIDLTQRALDARRVDQAKLYLAAAGAAFADLSYENGRHGD
                     ALWMYQEMPRRAK"
     gene            2478..4067
                     /locus_tag="PLY56_07450"
     CDS             2478..4067
                     /locus_tag="PLY56_07450"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOJ21353.1"
                     /translation="MLQAGASFLSPEPQVVLIDAFRRPFDTAVAAARTCYSARGIIRP
                     EEVAGDDAPDDEARERARARRDALAASIFRAGHHTVFQHAHLQFALSNVSRHFVWSFL
                     HAHPFYNSEQVSQRYVAVKPGNYAIPPLAGEALAVYEATIAAQFAAYEELGRMLLPAT
                     EAAYFRRFPGRRHQAPRWQGEIEKRALEVARYILPIATFTALYHTISALTLLRYHRVS
                     QLFDVPLEQRIVVGKMVDCLLAAAPESAPLLGEPVAPEETAEYSILAEPGGDPRAFRD
                     EFDTSLEGHRSRLVDWKAGNEAILAQSVREVLGLPRTRLSDEEAIRMALDSGRNPMLA
                     QTLNLTMHGKLTRCLVHPSYTFRKRLSHTADSQDQRHRLTPASRPVLQAHVDGQPDVI
                     VPDLIASDPACARRYADAVERSWEGVARLRSLGVAPEFALYLLPNGVAVRFTESADLL
                     GLRHKLAMRLCYNAQEEIWRVALDEARQIREVNPVIGSYLFPPCRLREMAGITPPCPE
                     GKRFCGARVWKLDLDAYQRDI"
     gene            4141..4596
                     /locus_tag="PLY56_07455"
     CDS             4141..4596
                     /locus_tag="PLY56_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOJ21354.1"
                     /translation="MNEESAPVPAAHRQVLTFRIANEWYALDVAQVRRVEKAPTIVAV
                     PSAPPALPGVFISQGQLIPAVDPRVILELPPATEESPEFVVIACHEEYVGGILVDWVD
                     EVTRVDAAAIEPPPVGAGALYASGQTLLQGRLVSLLRLEALFQAATGEL"
     gene            4597..>4848
                     /locus_tag="PLY56_07460"
     CDS             4597..>4848
                     /locus_tag="PLY56_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21355.1"
                     /translation="MPAERWLLLRAGSRRIAAPLEQVAGLAEAGDGGDASVSDAEIAL
                     SERLWGEQESVPGVSLRVHSDPGARVRVAAAEEISEECEL"
BASE COUNT          765 a         1553 c         1694 g          836 t
ORIGIN      
        1 cgcgatggtg ggtgcggatg accaggtcta cctggccgtg ggcgaggaga tggttctcct
       61 gagcgatgtc gcgctggtga ccgcctgaga ccgcctgctg caggggccgc agccttcacc
      121 ggcacgcatg ccgctgtggc tacgctggcg cgcgcctcct gtttcatcct tctcccggcc
      181 gtggtttgac ctgattcagc ccggccctgc cggggatctg gcgaggcacc cagaccgcgg
      241 gatcctgagg aatctggagt ctgtccaggc acgggccgag accaggaaga ccgtattccg
      301 cggcaaggtc gcccacatcc gtgggtgccc cgccaggatg caggatctcc accccgagcc
      361 cttctcccgg ctggaggatg ccaaccccta gcgcatacca ggcgtggaga gctaggttaa
      421 cggtatctct cctgagtagg cgcttcctcc ccatgacggt gagccctccc ggcgcctgct
      481 tctgggatgc ctggcgtcat tcccctgcgc aggcgatgat ccagagaggg ccggccgcgg
      541 gcgggagcct ccggcgtgtc atggggatgg gcgcacatgc cagccccggg gagcggaagg
      601 ggaggggagt tgagagagtg cgcggagacg gcaaatgcct acgaggagaa tccggcgcgg
      661 ggggagaaga tttgctatga gggtctgggg ccgacggccg gtgcaaaggc gccggtactg
      721 gctcacctgg gccgagcgga ggatgcactg ccatcctggc gttggcgagt tggccgcgct
      781 ggcaggtggc tgcatgcgcc ccgaagcggg gaaccgcttt acccaggagc gcggcagcgt
      841 cgggtggggc cccgatgggc gtccgtctga taattcagac ggacggcgct tctcccgaag
      901 gcgcgtcacc caatattgcc ggatggtcgg ggcacgagtg ggcgttctgc cttgccccag
      961 gcgtgattcg gtccggcaca cggaagaaac gagagtctat ggccaagatc ctagtggtgg
     1021 atgacgaacc gaatatcgta aagcttacct cttttcacct gcgccggcat ggccatgagg
     1081 tggtctctgc ggataacggg gccgcggcgc tggtgacggc gaaccaggag cgtcctgacc
     1141 tcatcatcct ggatgtgatg atgccggtca tggacggctt cgagacgctc cgctcgctgc
     1201 gcgccgcgga ggagacccgc gagatcccga tcatcatgct gacgtgtcgc aacctggatc
     1261 acgacatcgc gcacggcctg atggagggcg cggatcttta tatcaccaag ccttttgaga
     1321 tggacaagct gatgctcgcg gttcgccggc tgttggcggt cgccggggat gcggcaggca
     1381 caaccgattg acaggggagc acgtctttgt tatagtatgg atgggcgggc acgccggcgg
     1441 cgtggccggc tggccccctg gcgcctatcg caacctggag agaaggcact catgatatcg
     1501 tggcgcaacc tccgctctgt gtgtgccgtg acggcattgc ttctgacgct tccccttctc
     1561 ggtgccggct gcagtggctc gggcgaggaa gaaacgtcct ctccgaaccc cgacgccgcg
     1621 ggcatgggcg ggccgcctgc tggcatggag ggccctccag gagggccggg accgggtgat
     1681 cccgcgatgg caggaccggg agaccccgga atggatgctc ctggggcagc ggagcccgct
     1741 ggtggaggag cggcttcgga agaggccatc tccttcacgc agatgccgtt cgtcacccgg
     1801 cccaagcggg tgatggtccg ccagccgggc ggaaagctgg tggagcatgt ggagttccgc
     1861 taccatgcca aaggcgaccc cgaggcactg cttgcattcc tgaatccgtc gcccagcgca
     1921 gcgcccgatg agggctccgt ctggttcgtg cgcatgcccc atgccgccac ggcgggtaag
     1981 aagaccccgg gggagtggga gaacctcttc tgcacctacg tggtgccaga taacgagtcg
     2041 atcgaacgct ctcgccgcgc gcttcaggcg cggctgacgc aactgcggaa ccaggtgtac
     2101 cggcagaaga tttatacggt cgacctcctg gcgggagatc ggctgccgca gacggcgcag
     2161 gtcaacgcga tggatacggc ggcctcctac gtgcccgatc cgagtgctcg cctgagcctc
     2221 tatatcgacc tgacgcagcg cgctctggat gcacgccggg tggaccaggc gaagctgtac
     2281 ctggccgcgg cgggcgccgc gtttgctgac ctttcgtatg agaacggccg gcatggggac
     2341 gccctctgga tgtatcagga gatgccccga cgcgccaagt agccgcgtcc ggcacctctc
     2401 caaagccacg cggtgatgga accttgaggg cgggattggt ggcggcacgc cgccgcgatc
     2461 gacgaggagc gtgagtcatg cttcaggccg gcgcgtcctt cctttccccc gagccgcaag
     2521 tcgttctgat cgacgctttc cggcgcccat tcgatacggc tgtcgcggcg gcgcgcacct
     2581 gctattccgc gcgtggcatc atccgcccgg aggaggtagc cggtgatgac gcccctgatg
     2641 acgaggcgcg ggagcgtgcg cgcgcgcggc gcgatgccct ggcggcttcg attttccggg
     2701 caggccacca taccgttttc caacacgccc accttcaatt tgccctcagc aacgtctcgc
     2761 gccactttgt ctggtcgttc ctgcacgcgc atccgttcta caattcggag caggtcagcc
     2821 agcgctatgt ggccgtgaag ccgggcaact acgcgattcc gccactcgcg ggcgaggctc
     2881 tggcggtcta tgaggccacc atcgcggcgc agtttgctgc gtatgaggag ctgggccgga
     2941 tgctcctgcc agcgacggaa gccgcctatt tccggcgttt tcccggccgc cggcaccagg
     3001 cacctcggtg gcagggcgag attgagaagc gtgcgctgga ggtggctcgc tatatcctcc
     3061 ccatcgctac cttcaccgcc ctctaccaca ccatctcggc gctcacactc ctgcgctatc
     3121 accgcgtcag ccagctcttc gacgtgcccc tggagcagcg gatcgtcgtg ggcaaaatgg
     3181 tcgattgcct gttggcggcg gcgcctgagt ccgcccccct gctgggagag ccggtcgcgc
     3241 ccgaggagac cgccgaatac tccatcctcg cggagcccgg tggcgacccg cgcgcgtttc
     3301 gtgacgaatt tgacacgtcg ctggaggggc atcggtcgcg cctggtcgac tggaaggcgg
     3361 ggaacgaggc catcctggca caatcggtcc gcgaggtact gggcttgccg cgcacgcgtc
     3421 tctcggacga ggaagcgatc cgcatggcgc tggattcagg gcgcaacccg atgctggcgc
     3481 agacgctcaa cctaaccatg catgggaagc tcacccgttg cctggttcac ccctcctata
     3541 ccttccgcaa gcggcttagc cacaccgcgg attcccagga ccagcgccac cggctgacgc
     3601 cggcgtcgcg cccggtgctc caggcccacg tggatgggca gccggatgtg atcgtgccgg
     3661 atttgatcgc gtcggatccc gcctgcgcgc gccgttacgc ggacgcagtg gagcggagct
     3721 gggagggcgt ggcgcgcctg cggtcgctgg gagttgcacc ggagttcgcg ctttacctgc
     3781 tgccgaacgg cgtggccgta cgcttcaccg agtccgcgga cctgctcggc ctgcggcaca
     3841 agctcgcgat gcgcctctgc tacaatgcgc aggaggagat ctggcgggtg gcgctcgatg
     3901 aagcgcgcca gatccgcgag gtgaacccgg tgattggcag ctacctcttc ccgccctgcc
     3961 ggctgcgcga gatggccggg atcacacccc cctgcccgga gggaaagcgc ttctgcggcg
     4021 cgcgcgtgtg gaagctcgac ctggacgcgt atcagcgcga catctgagag aggccgggcg
     4081 gtctgctccc gccaggagat ggggcgcgaa cgtcgaaagc aggcagcgag gcatgcagtg
     4141 atgaatgagg agtccgcgcc agtaccagcc gctcaccgcc aggtcctgac ctttcgcatc
     4201 gccaacgagt ggtatgcgct cgacgtggcc caggtgcgcc gggtggagaa ggcccctacg
     4261 attgtggccg tgcccagcgc tccgccggcg ctgccgggcg tctttatctc tcagggccaa
     4321 ttgatcccgg ccgtcgatcc cagagtcatc ctggagctgc cccccgccac ggaggaatcc
     4381 ccggagttcg tggtgatcgc ctgtcacgag gagtacgtgg gcgggatcct ggtcgattgg
     4441 gtcgatgagg tcacccgcgt ggacgcggca gcaatcgagc cgcctccggt cggcgccggc
     4501 gccctctatg ccagcggcca gaccctcctt caggggagac tggtctcgtt gctccgcctg
     4561 gaggcacttt tccaggcggc cacgggagag ctgtagatgc cggcggaacg ctggctgctg
     4621 ttgcgcgcgg ggtcgcgccg gatagcggcg cccctggagc aggtggcggg actcgcggag
     4681 gccggcgatg gcggggatgc cagtgtctcc gatgcggaga tcgccctctc ggagcgcctc
     4741 tggggagagc aggagagcgt ccccggggtt agccttcggg tgcattccga tccgggcgcg
     4801 cgggtgcgtg tcgctgctgc cgaggagatc tcggaggagt gcgagctg
//
LOCUS       DAOSCV010000248         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11064_length_4831_cov_3.180486, whole genome shotgun sequence.
ACCESSION   DAOSCV010000248 DAOSCV010000000
VERSION     DAOSCV010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11064_length_4831_cov_3.180486"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PLY56_07465"
     CDS             <1..1821
                     /locus_tag="PLY56_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21356.1"
                     /translation="RGLRLADEPLSDRFVFATALGLGCVAYAIFALGALQLFRPVPML
                     LLLTGLLAWAAAPALALVREAWAGLRAGCSSRSLLSLALGGAVALLMGLALVAALAPP
                     AGNDWDGLSYHLADPAIYLRRGGIVPLFWESHSNFPFTVEMLYTVALLAKSAGAARAI
                     HWLYLVLACLTLAAAWRRLWPEDAPGARWLPPLLLCSSPIILWSATVAYIDLALMAYE
                     LLALYGVVLWWKTGRTGWLWAGAIALGFALGTKMTGLLFLALLAPLVLAAGLQAKESW
                     GGALRRVVTWGAIAVAIGAPWYVKSYLYTGNPVYPFFYHLFPNTRYWNADLAAIYAKH
                     QDDDFGLGDTLAHLVSAPWDLLAHPDSFTDGAPAFRILFGSLGPAFLALAPIALFLRR
                     RDPLAFGSLLFAAAYGVLWFHMTQQTRHLLPVAPLLALAGARGLSALVDERRFARFPG
                     FGAAGVQVAHALLIGVLMAGPAWPVVTGRIPAHDYLRNALDIYPICEYVNEYLPEDAR
                     LLLIHEVRGFYLERNYLWGNEGHHAAIPWSGFRNEAEMRRYLHQELGVTHVLINHRIQ
                     PREARPEGWERTLWEAIRAGTLEPVMEERGYCVYAVQPQE"
     gene            1874..3286
                     /locus_tag="PLY56_07470"
     CDS             1874..3286
                     /locus_tag="PLY56_07470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ21357.1"
                     /translation="MARKTSKKTGSGKEQVPPGPARTPVSGRDLLRERPAWLVALLAV
                     YFVLGGLYLATVPFGHAPDESAHLLYVEQLASGQLPVFRAEDRANFEAHQPPLAYLVA
                     LPAYLLAGGEGDAARYAVRLVSLVLGAVVIWLVAAAVRLWLPGQPGVALAAGAVAAFM
                     PMRLAVFSSFSNDPLTETLFCAVLFLLGLGLCNGFDRRRCLWVGALIGLGLLTKTTCI
                     LLLPVALVALFLEWRRHRSRAREYLANAGLLAGVAVAIGLPWMARNVVLYGDPFAWKA
                     FEAFFGDRATPEYWMARQGLSFRQYIFFWVPAFTIRSFWGVFDHMNLWMGGPVRFPPP
                     EIKPHLIPLGTPDAPIYLVLTRVAQLLAVGAVVAGIRWWRSKPEAWQRDIALTWGLCA
                     ALIVAAFFRFNTQYYQAQARYLYPAVLPIAATMAAGWLVAWPERLRQMGALLAAALLA
                     GLAAHAWHGTLLRVFGKVGW"
     gene            3286..3978
                     /locus_tag="PLY56_07475"
     CDS             3286..3978
                     /locus_tag="PLY56_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ21358.1"
                     /translation="MAPLVLTVLMPAYNEIDTIREAIRRVCETDLGEGISKEVIVIDD
                     ASTDGTREVLAEIPGIIVVKHARNQGKGAAIRSGLARATGDVVVIQDADLEYDPADLR
                     AMLTPIRHGKAAVVYGSRFLKGRPRMRLANYIANRILAWSATLLFGQRITDEATCYKM
                     FRTDVLRAMNLRAERFEFCPEVTAKARRAGYRIVEVPISYQARTLAEGKKITWRDGLE
                     AIWTLVKYRFVR"
     gene            4026..4574
                     /gene="lptC"
                     /locus_tag="PLY56_07480"
     CDS             4026..4574
                     /gene="lptC"
                     /locus_tag="PLY56_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOJ21359.1"
                     /translation="MKRNRALLAMVAGLLMLGGAGCHRRPSVPAQRPPEPPRPERPFE
                     PRLRASGVSWTLSDPEGLPVWEVKAKSGSGAAAEGIAELREVRCNVYSAGKLALETQA
                     AQVKADYSRKRLALYGGVDARTPDGTRSFHCERVSLTVEEEKRAVVDATGAVRLKVDG
                     VEMEGARLITNPRLDEGQLLSQ"
BASE COUNT          682 a         1559 c         1720 g          870 t
ORIGIN      
        1 cgcgggctga ggctggcaga tgagcctctt tccgaccggt tcgtcttcgc caccgctctc
       61 gggctcgggt gcgtggccta cgccatcttt gcccttggcg cgctccagct cttccggccg
      121 gtgccgatgc tcctgctact caccggcctc ctggcctggg ccgccgcgcc tgcgcttgcc
      181 ctcgtccggg aggcctgggc cggtctgcgt gccggctgca gctctcggag cctcctctcc
      241 ctggccctcg gcggtgccgt tgccctcttg atggggctgg cgctggtcgc ggctctggct
      301 cccccggctg gcaacgactg ggacggtctc tcctatcacc tggccgatcc ggcgatctac
      361 ctgcgccgcg ggggaatcgt gccgctcttt tgggagtcgc actcgaattt ccccttcacc
      421 gtggagatgc tctacacggt ggcgctcctg gccaaaagcg cgggcgcggc acgggcgatt
      481 cactggctct atctggtact cgcctgcctg acgctggcag cggcatggcg ccggctctgg
      541 cccgaggatg cgcccggcgc gcgctggctt ccgcccctgc tcctctgctc gtcgccgatc
      601 atcctctgga gcgccacggt ggcctacatc gacctggcgc tgatggccta cgagctgctg
      661 gcgctctacg gcgtggtcct ctggtggaag acggggcgca cgggctggct ttgggcggga
      721 gcgatcgccc tcgggtttgc cctcggcacg aagatgaccg gcctgctctt cctggcgctg
      781 ctggcgcctc tggtactggc ggccggcctg caggcgaagg agagctgggg gggcgctctg
      841 cggcgagtag tgacatgggg ggccatcgcc gtggccatcg gcgcgccatg gtacgtgaaa
      901 agctatctct acacgggtaa cccagtctat cccttcttct accacctatt tccgaacacg
      961 cgctactgga acgcggatct cgccgcgatc tacgcgaagc accaggatga cgatttcggc
     1021 ctgggcgaca ccctggccca tctggtgtcc gcgccgtggg acctgctcgc gcatccagac
     1081 agcttcaccg atggcgcccc ggcattccgc attctcttcg gctcgcttgg ccccgctttc
     1141 ctggcgctcg cacctatcgc gctcttcctg cgccggcgcg acccgctcgc gttcgggtcg
     1201 ctccttttcg cggcagccta cggcgtgctc tggttccata tgacgcagca gacgcgccat
     1261 ctcctgccgg tggcgccgct gcttgcgctc gccggggcac gcgggttgag cgcgctggtt
     1321 gacgagcgcc ggttcgcgcg cttcccgggg tttggcgccg cgggcgtcca ggtggcgcac
     1381 gcgctcctga tcggcgttct gatggccggg ccggcgtggc cggtcgtcac cgggcgcatt
     1441 cccgcgcacg actacctgcg gaatgccctc gacatctatc cgatctgtga gtacgtgaac
     1501 gagtatctgc cggaagatgc gcgcttgctc ctgattcacg aggtgcgcgg gttctacctg
     1561 gagcgcaact acctgtgggg gaacgaaggc caccacgccg ccattccttg gtccgggttc
     1621 cggaacgaag ccgagatgcg ccgctacctg caccaggagc tgggcgtgac gcacgtgctg
     1681 atcaaccacc ggatccagcc gcgagaggct cgtcccgagg ggtgggagcg cacactctgg
     1741 gaggcgatcc gcgcgggaac cctggaaccc gtgatggagg agaggggtta ctgcgtctac
     1801 gcagtccagc cacaggaatg agggagcagg gaggaagggc tccccgcttc cccgcggggc
     1861 gatagtgaac gcaatggcgc gcaaaacgtc caagaaaaca gggtcgggta aggagcaggt
     1921 cccgccgggg cctgccagaa cgccagtctc aggcagggat ctcctccggg aacgcccggc
     1981 gtggcttgtc gcgctcctgg cggtctattt cgtgctggga gggctctacc tggcgacggt
     2041 gccgttcggg cacgcgccgg atgagagcgc gcacctgctt tacgtggagc agcttgccag
     2101 tggccagctg ccggtcttcc gcgcggagga tcgcgccaac ttcgaggcgc accagccgcc
     2161 gcttgcttac ctagtggcgc tgccggccta tctcctcgcc gggggcgagg gtgacgcggc
     2221 gcgctacgcg gtgcgcctgg tcagcctggt cctgggcgcg gtggtgatct ggctggtggc
     2281 tgcggcggtc cggctgtggc ttccggggca gcccggggtg gcgctcgcag caggcgccgt
     2341 agcggcgttc atgccgatgc gcctggcggt cttctcctct ttcagcaacg acccgctgac
     2401 ggagacgctc ttttgcgcgg tgctgttcct gctggggttg ggcctgtgca acggctttga
     2461 ccggcggcgc tgcctgtggg tgggcgcgct gatcgggctg ggcctcctga cgaagacgac
     2521 ctgcatcctg ctgctgccgg tggcgctggt ggcgctcttc ctggagtggc gccggcaccg
     2581 gagccgggcg cgggagtatc tggcgaacgc cggccttctg gcgggggtcg cggtcgccat
     2641 cggtctcccc tggatggcgc gcaacgtggt gctctatggc gatcctttcg cctggaaggc
     2701 cttcgaggcg ttctttggcg atcgggcgac cccggaatac tggatggccc ggcaggggct
     2761 ctcgtttcgc cagtacatct tcttctgggt gccggctttc accatccgca gcttctgggg
     2821 tgtgtttgac cacatgaacc tgtggatggg cgggccggtc cggttcccgc ctccggagat
     2881 aaagccgcac ctcatcccgc tgggcacccc ggacgcgccg atctacctgg tgctgacccg
     2941 cgtcgcccaa ctgctggcgg tcggggcagt cgtggcaggc atccgctggt ggcggagcaa
     3001 accggaggcg tggcagcgcg atatcgccct gacctggggg ttgtgcgcgg cgctaatcgt
     3061 cgccgcgttc ttccggttca acacgcagta ctaccaggcg caggcgcgct acctctaccc
     3121 ggcggtgctt cccatcgccg cgacgatggc ggcggggtgg ctggtggcgt ggccggagcg
     3181 cctgcgacag atgggcgcct tgctcgccgc ggcgctgctc gccggcctgg cggcgcacgc
     3241 gtggcacggc acgctcctgc gcgtctttgg aaaggtgggg tggtagtggc gccgctcgtc
     3301 ctgacagtcc tcatgcctgc ctataacgag atcgacacaa tccgggaagc gatccggcgg
     3361 gtgtgcgaga ctgacctggg cgagggcatc tccaaggagg tcatcgttat cgatgacgcg
     3421 tccaccgacg gcacgcgcga ggtgctggcc gagataccgg gcatcatcgt ggtgaagcac
     3481 gcgcggaacc aggggaaggg cgcggcgatc cgctccgggc tggcgcgcgc caccggcgat
     3541 gtggtggtca ttcaagatgc cgacctggag tacgatccgg ccgatctgcg cgcgatgctc
     3601 acaccgatcc ggcatgggaa ggcagcagtg gtttacggct ctcgcttcct gaagggccgg
     3661 ccccggatgc gcctggcaaa ctacatcgcg aaccggatcc tggcctggag cgcgacactg
     3721 cttttcggcc agcggatcac ggacgaggcg acctgctata agatgttccg gaccgacgtc
     3781 ctccgtgcca tgaacctgcg cgcggagcgc ttcgaattct gcccggaggt gacggccaaa
     3841 gcgcggcgcg ccggctaccg catcgtggag gtgccgatct cttaccaggc aaggacgctt
     3901 gccgagggga agaagatcac ctggcgcgat ggcctcgagg cgatctggac cttggtgaag
     3961 taccgctttg tgagatagcg gcgggtggtg acactgggga agcacgcgtg ttgtctaata
     4021 gaaagatgaa gagaaaccgc gccctcctgg cgatggttgc ggggttgctc atgctgggag
     4081 gggcaggctg ccaccggcgt ccatccgttc ccgcacagcg cccgccagaa cccccacgcc
     4141 cggagcgccc tttcgagccg cgcctgcgcg cgtcgggagt ctcctggacg ctctccgatc
     4201 cggagggcct gccggtctgg gaggtgaaag cgaaaagcgg gtcgggagcc gccgccgagg
     4261 ggatcgccga gctgcgcgag gtgcgatgca acgtgtactc cgcggggaag ctggccctgg
     4321 agacgcaggc ggcgcaggtg aaggcggatt actccaggaa gcgtctcgcg ctgtatggcg
     4381 gcgtggacgc acgcaccccg gatgggacgc gctcgttcca ttgcgagcgg gtttcgctga
     4441 cggtggagga ggagaagcgc gcggtggttg acgcgaccgg tgcggtccgc ctgaaggtgg
     4501 atggcgtgga gatggagggg gcgcgcctga tcacgaaccc gcggctcgat gagggccagc
     4561 tgctctcaca ataagcgacg gggatcgcga cacccgtcgc tggcagcctg atttcacgcg
     4621 cgcccgatct cttcgcaggc aagaagagtg cctcggaaca gatagagaat acaggagtgg
     4681 gaatcatgaa ggcagataga aaagcgctta tggcgctggt ggccgtgttg gccggcgtgt
     4741 gcgccgtgca tgcggcgccg gccaagaagc cggccagcgt gaagtggccc ggcggccagg
     4801 tctccgcgga cgggcagatc ggcttcaaga c
//
LOCUS       DAOSCV010000249         4827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11080_length_4827_cov_3.039816, whole genome shotgun sequence.
ACCESSION   DAOSCV010000249 DAOSCV010000000
VERSION     DAOSCV010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11080_length_4827_cov_3.039816"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="cas5"
                     /locus_tag="PLY56_07485"
     CDS             complement(<1..327)
                     /gene="cas5"
                     /locus_tag="PLY56_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOJ21360.1"
                     /translation="MRVTRVEVEGPTTSFRYPHFLVGRQPTYEMPPLATLYGHICSAV
                     GEWLDPSGLRIGFRFTYTAKADDKEQIHIITASAPRAKLPGTDMPANVETGAPQSIQI
                     REYLFEP"
     gene            complement(415..1470)
                     /gene="cas7i"
                     /locus_tag="PLY56_07490"
     CDS             complement(415..1470)
                     /gene="cas7i"
                     /locus_tag="PLY56_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HOJ21361.1"
                     /translation="MAHLNGLWLIDAPASALNNLGQAEGGRTDNVVGVKHIRTREGAS
                     PYVSAQAFRYWLRTTLETHVPEWKAAPMFREKKVAYTDGDPIKWWDDDLFGYMRAASK
                     KADAKEAREASPAENQTPADIELTRTSPFKVGTLVSIAPVNITDDFGVMARHEGDPVP
                     HEHQFYRTTLKGLFALDLHACGTFWHRYKTGFRNLDTTRKKEAEELGLEPFDEGRAYR
                     LPVEQRVERIAALLAGLSQLEGGAKQALHYTPVAPSWFIMAVTEGGNNIFQHVVGADS
                     RGLPLVKTEALLEAVRVHEDQLMSPIYVGWVRGYLDDQREKVCTALSEWQVVVDHPRR
                     VFDRLIADLKEHPEWLA"
     gene            complement(1482..2900)
                     /gene="cas8a1"
                     /locus_tag="PLY56_07495"
     CDS             complement(1482..2900)
                     /gene="cas8a1"
                     /locus_tag="PLY56_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HOJ21362.1"
                     /translation="MLRYTGHPFVDVGAATLTAFAEKERPEHLTSDDLQVAAEYMEKV
                     YMGKHMNSFLVCVFPNSAYVNPTSGADKKQQFLDFHLYGFRQAATPEAPPCVACGQRA
                     LGPTFRQHVPLLTGEDVLNFFPNAQPGLPLCGACLLAIAALPLGALKCSGRALILHAD
                     DDSITLDFAREHLERNRRLLQLAGQSNSKYPDAKEVRTRLMEQLCAWAERQRRRGSET
                     RALSLTAYHFTNSGQGPDIDIYHLPSQLVDFVRTATGARYASAWNAIVRRAWWAGRDA
                     SDAADTRNVFYEDLLRLPDNAPLFIRRYFLREAYRAAPKDDPRGGYNLAAELDVVSWP
                     LTDLFLRKVVNMDRSRIEAIRALGDRLAEYIESQNDRRFWRRLYTADKYYILRNALIK
                     ASESEIKAGRPPLITFDDFILVFEEGEELVRTDWGLARDLALIRVIEQLHARQWFAKN
                     PEALSEESDTSESDEEKEAATA"
     gene            complement(3140..4123)
                     /locus_tag="PLY56_07500"
     CDS             complement(3140..4123)
                     /locus_tag="PLY56_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ21363.1"
                     /translation="MTRPSTRPPIARLHWWDAALRENRYPTIAECMERFEISTRTAYR
                     DLDYLRYQMNAPLAYDRQRRGYCYTDPGFLLPAVRLSDGEIIALFLAEKILKQYRGTP
                     YADYLREAFDKIRAALPDEVSLDPSGLSEALSFDLGPARPVEVDVFQTLLRAVRERRS
                     IDIVYFSQARGVETERRVDPLHLHAFSGDWYLIAFCHLRGEVRDFALSRIRRLEGTDE
                     RFSPPAGFDRDAYLGQAFGLLKGGTPQEVAVWFDAYQARWVRERQWHPTQVLEEQPGG
                     ALILRMTVPVTDDVLRWVLSYGSHCRVLAPAELQAKVRDEVEKMRAGLMGS"
     gene            complement(4192..>4827)
                     /gene="cas6"
                     /locus_tag="PLY56_07505"
     CDS             complement(4192..>4827)
                     /gene="cas6"
                     /locus_tag="PLY56_07505"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOJ21364.1"
                     /translation="LVGSGNEFLPYFLLALNELASAGIGAGRSAVAVERVVAYHPFTG
                     ESEPVWEGRNTPVRSTGIAVTWEDAVARAAELPEETATIRFLTPTCLKVGGTVLSRPD
                     FPALVRCLLRRVSRLAQGYCGGASPLDYASLIRQAEGIRIVASDLHERQWERHSYRQQ
                     RRIPSRGITGTITYAGPLRPFLPLVLIGGLVHIGDNAALGQGRYEVVVPGG"
BASE COUNT          850 a         1572 c         1490 g          915 t
ORIGIN      
        1 gggctcaaag aggtactcgc gaatctggat gctctgcgga gcgcctgtct ccacattcgc
       61 gggcatatcg gtgccaggca gcttcgcccg cggggccgag gccgtgatga tatggatttg
      121 ctccttatcg tccgccttgg ccgtgtaggt gaaccggaac ccgatcctca gccctgaagg
      181 atccagccac tcgcctacgg cgctgcagat gtgaccgtac agcgtggcca gcggcggcat
      241 ctcataagta ggctgccggc ccactaggaa gtgcgggtag cgaaacgaag tggttggccc
      301 ctccacctca acacgtgtga cgcgcatggc acatcccttc tgccggttcc tgattgtgcc
      361 ccgggtaggc gaaacccgcg tgggtccggt tcctaccttc atgggcagcc accctcatgc
      421 cagccactcg gggtgctcct tcaggtctgc gatcagccgg tcgaacacgc ggcggggatg
      481 gtcgacgacc acttgccact ccgacagggc ggtgcagacc ttttcgcgct ggtcatcgag
      541 atacccgcgc acccaaccga cgtagatcgg agacatcagt tgatcctcgt gcacccgcac
      601 cgcctccaag agcgcttcag ttttcactaa cggcaggccc ctggaatccg cgccgaccac
      661 gtgctggaag atgttgttgc ctccctcggt caccgccatg atgaaccacg atggagcgac
      721 cggcgtgtag tgtagcgcct gcttcgcgcc accctcgagc tggcttagcc cggccagcag
      781 agccgcgatc cgctccactc gctgttcgac tggcaggcga taagcccggc cctcatcgaa
      841 cggttccaga ccaagctcct ccgcttcttt cttgcgcgtt gtgtccagat tacggaagcc
      901 ggtcttatac cggtgccaga aggtgccgca ggcatggagg tcgagggcga agaggccctt
      961 cagggtcgtt cggtagaact ggtgctcatg gggtaccggg tctccctcat gccgggccat
     1021 gaccccgaag tcgtcggtaa tgttgaccgg tgcgatggaa accagggttc ccactttgaa
     1081 cggcgaggta cgcgtcagtt cgatatccgc tggagtctgg ttctcggcgg gcgatgcctc
     1141 ccgagcttcc ttcgcgtcgg cctttttcga tgccgccctc atatagccaa acaggtcatc
     1201 gtcccaccac ttgatcgggt ccccatccgt gtaggcgacc ttcttctccc ggaacatcgg
     1261 tgccgccttc cactcgggca cgtgagtctc cagcgtcgtc cgcagccagt aacggaacgc
     1321 ctgtgcggac acataggggg atgcgccctc gcgcgtgcgg atatgcttca ctcctacgac
     1381 attgtcggtg cgaccaccct ccgcctggcc caggttgttc agggccgagg ccggagcatc
     1441 aatcagccaa agtccgttca ggtgtgccat ggttttcctc ctcacgcggt agctgcttct
     1501 ttctcctcgt cagattccga cgtgtccgat tcctcagaca gcgcctcggg gttcttagca
     1561 aaccactgcc tggcatgcaa ctgttcgatg actcggatca gcgccaggtc tcgggccagc
     1621 ccccagtctg ttcgaacaag ctcttcgccc tcctcgaaga cgagaataaa gtcgtcgaaa
     1681 gtgatcagag gaggacgacc ggctttgatc tcactctcag atgccttgat cagggcattc
     1741 cgaaggatgt agtatttgtc ggcggtgtag agcctgcgcc agaagcgccg gtcgttctga
     1801 gactcgatgt actcggctag tcggtctcct agagcgcgga tagcttcgat gcgggatctg
     1861 tccatgttta ccacctttcg gaggaacagg tcggtcaggg gccaagagac gacatccaac
     1921 tcggcggcga ggttgtaccc gccccgcgga tcatctttgg gcgctgcgcg atatgcctct
     1981 cgcaggaagt agcgccggat gaagagcggc gcgttgtccg gcaaccggag gagatcttcg
     2041 tagaaaacgt tccgggtgtc cgctgcatcc gaggcgtcgc ggccagccca ccaggctcgc
     2101 cggacaatcg cgttccacgc ggaggcgtag cgtgctcccg tggccgtcct tacgaaatct
     2161 accagctgcg acgggagatg gtagatatcg atgtctggcc cctggcccga gttggtgaag
     2221 tggtacgccg tgagcgagag tgcccgcgtt tcgcttcctc gcctgcgctg gcgttcggcc
     2281 caggcacaca gctgctccat caaccgggtg cgcacctcct tggcgtcggg gtacttactg
     2341 ttgctctgcc cggccagctg tagcaggcgt cggtttctct ccagatgctc gcgggcgaag
     2401 tctagcgtta tcgaatcgtc atcagcgtgg agaatgagcg cgcgtccact gcatttgagc
     2461 gctcccagtg gcagcgccgc gatcgccaag aggcaggcgc cgcatagggg aaggccaggc
     2521 tgcgcgttcg ggaagaagtt cagcacgtct tcgccagtga gaagcggcac gtgctggcgg
     2581 aatgtggggc ccaacgcccg ctgtccgcac gccacgcatg gaggtgcctc cggcgtcgca
     2641 gcctggcgga agccgtaaag atggaaatcg aggaactgct gcttcttatc cgctcccgat
     2701 gtggggttga cgtacgcaga atttgggaag acgcacacca ggaagctgtt catgtgcttt
     2761 cccatataca ccttttccat gtactctgca gccacctgca aatcatcact cgtcaggtgc
     2821 tccggtcgct ctttctccgc gaacgccgta agcgtggccg cgcctacgtc cacaaatggg
     2881 tgtcctgtgt aacgcaacat ctcgctgtcc cttcaccctg cccgaatcgg caaagggcca
     2941 gcccggccca gtgcccggca aaccgcaact tcatcttagc atagttactc tgccatttgc
     3001 tgtcactctg acaggggagt gtgtaccata ggggagccta ttggcctctc cgcatttcct
     3061 tatgctgtct ctctagtcaa atgggcctgg cggtgagtac ttcccgtgcc gttatacgcg
     3121 cgggcgttac agcttgcggc tatgatccca tcaaccccgc gcgcatcttc tccacctcat
     3181 cccggacctt cgcctgcagc tcggcggggg ccagaacgcg gcagtggctg ccgtaggaga
     3241 ggacccaccg gagcacgtcg tcagtcacgg gcacggtcat gcgcaggatc agggcgccgc
     3301 cgggctgctc ctcgagcacc tgcgtcggat gccactggcg ctcgcgcacc cagcgcgcct
     3361 ggtaggcgtc gaaccacacc gccacctcct gcggcgttcc ccctttcaac aggccaaacg
     3421 cctggcccag atacgcgtcg cggtcgaacc ccgctggcgg tgagaagcgc tcgtcggtcc
     3481 cctcgagccg gcggatccgc gagagcgcga agtcgcgcac ctctccccgc aagtggcaaa
     3541 acgcgatcag gtaccagtcg ccgctgaagg catgcaggtg aagcgggtcg acgcgccgct
     3601 ccgtctccac gccgcgcgcc tggctgaagt agactatgtc gatgctccgg cgctcacgca
     3661 ccgcgcgcag cagggtctgg aacacgtcca cttcgaccgg acgcgccggg ccgaggtcaa
     3721 atgagagcgc ctcgctcagc ccggatggat cgagcgacac ctcgtccggc agcgccgcac
     3781 ggatcttatc gaatgcctcg cgcagatagt cggcatacgg cgtcccgcga tactgcttca
     3841 ggattttctc cgccaggaac agcgcgataa tctccccatc cgagaggcga acggctggca
     3901 gtaagaaccc gggatcggtg tagcaatagc cgcgccgctg cctgtcgtag gcgaggggag
     3961 cgttcatctg atagcgcagg tagtccagat cgcgatacgc ggtccgcgtg ctgatctcaa
     4021 accgctccat gcactcagca atggtgggat agcggttctc gcggagcgcc gcgtcccacc
     4081 aatggagccg tgcaattggc ggtcgggtgg atggtcttgt catcgccccc tcctcgcgag
     4141 cggcgacgtg aaagagccgc ctcgcgcgtg gatggagttg cagcacccgg attagccgcc
     4201 tgggaccacg acttcgtagc gcccctggcc gagtgccgcg ttatccccga tatgcaccag
     4261 gcccccgatc aaaacgagcg gcagaaacgg gcgcaacggc ccggcgtagg tgatcgtgcc
     4321 ggtgatgccg cgagacggga tccgccgctg ctgccggtag ctgtgccgct cccattgccg
     4381 ctcgtggagg tcggaagcca caatccggat tccttccgcc tggcggatga gcgaggcgta
     4441 gtcgagtggg gaagcgcccc cgcagtagcc ttgcgcgagc cgggagaccc ggcgcaagag
     4501 acagcgcacg agcgcgggaa agtccggcct ggagagcacc gttcctccca ccttcaagca
     4561 ggtgggcgtg aggaagcgga tcgtggccgt ctcttccgga agctccgccg cgcgcgccac
     4621 cgcgtcttcc cacgtcacgg cgattccggt gctccggacg ggcgtatttc gcccctccca
     4681 gaccggctcg gactctccag tgaacgggtg gtacgcgacc acgcgctcga cggcgacggc
     4741 gctgcgcccg gcgccgatgc cggcgctcgc gagttcattc agcgccagaa ggaagtaggg
     4801 gagaaactcg ttgccgctcc cgacgag
//
LOCUS       DAOSCV010000250         4820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11108_length_4820_cov_3.049528, whole genome shotgun sequence.
ACCESSION   DAOSCV010000250 DAOSCV010000000
VERSION     DAOSCV010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11108_length_4820_cov_3.049528"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="PLY56_07510"
     CDS             complement(<1..1723)
                     /locus_tag="PLY56_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOJ21365.1"
                     /translation="MRIVRETCVPRPEVLHGELDDAIFAADFGHVIGGNAPQVYQDPC
                     LFFRNTHPAEPLRHLVRTVFDRLAEPDEAGALLRLRTGFGGGKTHALIALWHLARHAS
                     ESTLGTDLLPAAGRPSAVAVAGFDGEKAGTAVVARHDGLETHSLWAELAWQLGGTDGY
                     AVMRDVDDPEKSPDSGLIRDVLPAKPVLILLDEVVKYMAQLSEHARGCLLRFLGALSA
                     EITARKQAGLFITDPAGQTAYLTETGLLNQALADARRLDDELGRRFTDLDPIGKESVQ
                     VIARRLFEEIEKAAADQASAEYHQLYQRVAAEHPGSIPAEAAGMGYADRIVQCYPFHP
                     RLLEVAQNRLGALERFQKSRGTLRLFARILRDIWESESNITLISEGDLNWSSPRIQQD
                     LLERLDRASLKAAVDADVHDHAGQLDGEYSTDIHRRVASALLLESLQEPSRPAVTKEE
                     LTLAVLRPTEVGYDPGEALDRLMERCWHTYSDATGQRYHFGYQPNNVKRIEERMAAPG
                     MEENARQRVRTMAQQYFRGHIFTLCPFPSAPSAVPDTQTPKLVLADSEKQAQEICDWA
                     NDSDPQAK"
     gene            2691..2906
                     /locus_tag="PLY56_07515"
     CDS             2691..2906
                     /locus_tag="PLY56_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21366.1"
                     /translation="MDLTGVFLGCGIADEDRGGYRLTISAAALAQHVDALQIAASNCP
                     ADYVGVVDDFREVITICSEEVGRLIVV"
     gene            complement(4217..4480)
                     /locus_tag="PLY56_07520"
     CDS             complement(4217..4480)
                     /locus_tag="PLY56_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21367.1"
                     /translation="MDRPYWDAEKPLVIQTERNCLRFFEKAERLQVGFAPYVNRRGEE
                     RMVKAVTLNLAALKEADRDTIIAAAELFEKLAGELRREADAKR"
BASE COUNT          780 a         1516 c         1611 g          913 t
ORIGIN      
        1 tcttcgcctg ggggtccgag tcgttcgccc agtcgcagat ctcctgcgcc tgcttctcgg
       61 agtcggcgag gaccagcttg ggcgtctggg tgtctggcac cgcggaggga gcgctgggga
      121 aggggcacag cgtgaagatg tggcctctaa agtattgctg agccatcgtc cggacgcgct
      181 ggcgggcgtt ctcttccatg ccgggcgcgg ccatgcgctc ctcgatccgc ttgacgttgt
      241 tcggttggta gccgaagtgg tagcgctggc cggtagcgtc agagtaggtg tgccagcagc
      301 gctccatgag gcggtccagc gcctcgccgg ggtcgtagcc gacctcggtg gggcggagga
      361 cggcgagtgt gagttcctcc ttggtgacgg cgggcctgct tggttcctga aggctctcga
      421 ggaggagggc ggaggcaacg cgccggtgga tatcggtgga gtactcgcca tcgagctggc
      481 cggcgtggtc gtgcacatcg gcatcgacag cagccttgag ggatgcccgg tcgaggcgct
      541 ccaggaggtc ttgctggatg cgggggctgc tccagttgag gtcgccctcg ctgatgaggg
      601 tgatgttaga ctcgctctcc cagatgtcgc ggaggatgcg ggcgaagagg cggagggtgc
      661 cccggctctt ctggaaccgc tccagggcgc cgagccggtt ttgggccacc tcaaggaggc
      721 gcgggtggaa ggggtagcac tgaacgatgc ggtcggcgta gcccatgccg gcagcctcgg
      781 cagggatgga tcctgggtgc tctgcagcca cgcgctggta gagctgatgg tactcggcgg
      841 aggcctggtc ggccgcggcc ttttcgatct cctcgaagag gcggcgggca atgacctgga
      901 cagactcctt gccgatggga tcgaggtctg tgaagcggcg gccgagttcg tcgtccagcc
      961 gccgggcgtc cgccaacgcc tggttgagca atccggtctc ggtcaggtag gcggtctggc
     1021 cggcgggatc ggtgatgaag aggccggcct gcttgcgggc ggtgatctca gcggagaggg
     1081 cgccgaggaa gcggaggagg cagccacgcg cgtgttcgga gagctgcgcc atgtacttga
     1141 cgacctcatc gagcaggatg aggactggct tggcaggcag cacgtcgcgg atgaggccgg
     1201 agtcaggcga cttctctgga tcgtccacgt cgcgcatcac tgcgtagcca tcggtgccac
     1261 cgagctgcca tgccagctcg gcccagaggc tatgggtttc gaggccgtcg tggcgagcga
     1321 ccactgcagt gcctgccttc tcgccatcga aaccggcgac ggcaacggcg gagggcctgc
     1381 cggctgcagg gaggagatcg gtgcccagcg ttgactcaga cgcgtggcgg gcgaggtgcc
     1441 acagggcgat gagggcatgg gtcttgccgc ctccgaaccc ggtacggagg cggaggaggg
     1501 cgccggcctc gtccggctcg gccagacggt caaacacggt acggaccagg tgcctgaggg
     1561 gttctgccgg gtgagtattg cggaagaaga ggcaggggtc ctggtacacc tgcggggcgt
     1621 tcccgccgat cacgtgtccg aagtcggcag cgaagatggc gtcatcaagc tctccgtgca
     1681 gcacttctgg ccgaggaacg caggtctcgc gaacaattct catcggttcc cctctcattg
     1741 tggtgcattg ttggggcacg ggtgctggcc ggcacgcaca ggaacgggtg cagggcggag
     1801 tgccagcgca ggaggagcgt ccgggcgccg gcctcccggg tgccgcgcat acccctattc
     1861 tggggcaggg agggcagctt cctgcgggcc caggagacaa tgacacattc tgccccttgt
     1921 gccgcttcgc acgcgcctgg agctcgctac ggcaaccact ttggagaagg ggttcggcgc
     1981 gccttcccct tttcccatcg cgaacggctg tacccctccc gcccaccagt gtggaaagtg
     2041 ccgccgcgaa gccccagcga ggcagagcgg ccgtgcagca atgggccggg cgtggcgagc
     2101 ccgagcatgt gccgcttggc ttccttggca cattcgttcc tgccggacgg gtcgcgcgct
     2161 ccaacccggt ccgctgctgc agatcgagtg tccaccagtt cgaggcgacg tccgcagggc
     2221 gacctggagg ccctacggcg cgtgaccaga gaaagcaact gccgttgctg atggaacagc
     2281 aggaaaagga tcattgccgg cgaaaggaag ccagcaatga agaggcccgg ggggtaagct
     2341 cgggcctggt gaacggagca ggttgcgccg cgaactccgt ggttccaccc actggttcca
     2401 cccgcacacg agtggacagt gctctacttc ggcaaccctc gcggagttcc tcctgctctt
     2461 cttcaccagg ttcgttccac cccggcccgt gtgcaggcgc gcgggaagtg accgcttgac
     2521 tcggtgagtc actgaccgct ttcttcgccg gttccgagac ggcgcggagg tgccaaaaac
     2581 ccgcatggta cctggattct tccccgaaaa gcgggggagt tcatgacacg aacaccgacg
     2641 ggggtctgga cgattaggaa tggaacgatg ttctggttgt agacccgtcg atggatctca
     2701 cgggcgtctt cctcggttgc ggcatcgcag acgaagaccg tgggggttac cgcctcacca
     2761 tctcggcggc tgccttggcg cagcacgtag acgcccttca gattgcagcc agcaactgcc
     2821 ctgcggatta cgtgggcgta gtcgatgact tccgagaagt catcaccatc tgttctgagg
     2881 aagttgggcg actgatcgta gtgtagcagt tctagaacat cagacatggc aacaccctcg
     2941 gcttggaccc ggcagagtgt gtcactaccg gggcacaccc accgcgcccg ccgaactcac
     3001 cgcccatgaa acatctgccg ctgacaggcc actaagggcc aaccggatct tagatgtatt
     3061 cgctgccata ccatattctc cttgcgctcg tctgtggcaa ttggtgtctg catcgccgcc
     3121 atctgctgcg caagcgttcc tcacctatcc caccgtcggc tcggtcacgg ctgtaccggt
     3181 gtcaggcacg ttgctggttg acgatgcgaa gtggcgcccg aagaaccgcc tgccgctcct
     3241 cgcagccaag cggaaccgtg ttcctcggaa ggtggtcttc gcgaatcggg gcgccggccg
     3301 gccagtcacg aatcggcagg gtggcagcca tcgcactgat gcgcttgggt ggggttaggc
     3361 gtaagctacg atagtcgtca cgttacgtta cgccaccact ggcaacgcgc ctctcacttg
     3421 ccgtgagaag agggagtctc ttgctggcct tcctgcctgg ggatgaggcc ggcttccctc
     3481 atccgggcca gggctgcctc aaagcgccga cgatggtcac gagggcgggg gatgggcgtc
     3541 agacctgccg ccatccgtgc gcccatcgag atccatacga tcttgctccg cctgccgtgt
     3601 accaccgacc ggccgccacg cgctccgtga tagtgacaga cctcccaccc aggcttcgcg
     3661 tggcgtttgc accgctctcc ggttcgcttg cttgtcgctc tacactgcat tgaaaatcct
     3721 ccatgagcta atctgacatc catggggtta tgaagatgcc ctgttggggg ccgttttggc
     3781 ggatagtcca tggtcgcagt cgtgcccgtc aggatcgtag caccggagtt cgccgccgtt
     3841 cgtgcccgcc aagaccagag ctgccggttg cccttcggaa tcgtagaccg ctaccatgcc
     3901 gccccccagc cggccgatca tggccacaga gagccttccc tccgagccaa agagccgtaa
     3961 gagcggcaat ccgtctcggt ccttccccac atcgagcaga agccggccct cttcgtcaac
     4021 gacccgaagg ggggcgcgta gcaccgttgc ggcctgttcc tggggcgcct gtggcggctt
     4081 gcctcggcag gcatgtttcc gttcccggta tgcccgttgt cgggccgccg cgtctgggta
     4141 cactcgttgc cgtgccatca gttcctccgt ttcgtcacgt tacggcggca ttcatctgcg
     4201 gtgtaggtag ccccgctcac cgcttggcgt ccgcttcccg ccggagttcg cccgccagct
     4261 tctcgaacaa ctcggcggcc gctatgatgg tgtcgcggtc ggcttccttg agtgccgcga
     4321 ggttgagtgt cacggccttt accatgcgct cctcaccacg ccggttcacg tagggggcaa
     4381 agcctacctg tagtcgctcc gccttctcga agaaacgcaa gcagttcctc tccgtctgga
     4441 ttaccagcgg cttctcggcg tcccagtatg gacggtccat cagcttctcc tttcttcttg
     4501 ccttgaccgc tgtgcctgcc atcggcaggg accgcagcgg ccatccgatg cctttgggtt
     4561 gaccaactcc actccgcagg ccacgcacca ggccggctgt tcgcctggga gatagaccag
     4621 gagagagcgt tcctcgaagg ccagcacggg ggccgcctca gagccgatgt cccagtcttg
     4681 gcctggttca tactctatgc ccgcaggggc acacgatgga gcggcggcac cgtcggcggg
     4741 ggccgttcgt gccctgcgcc gttgctcggc caccactcgc agcgcaatct ccaagagcga
     4801 acccattgtc tctctcccta
//
LOCUS       DAOSCV010000251         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11123_length_4815_cov_4.226891, whole genome shotgun sequence.
ACCESSION   DAOSCV010000251 DAOSCV010000000
VERSION     DAOSCV010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11123_length_4815_cov_4.226891"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLY56_07525"
     CDS             <1..942
                     /locus_tag="PLY56_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ21368.1"
                     /translation="LLPAVKKSRFWAPRPKPAPEPLGELAVTAFAAYLSCPYRFYLSR
                     ILKLRDAADEAEEMDALLFGSLVHDTLRALATGTARDSTDPSEIAAFLEADLERRICE
                     QFGGEPAPAIRLQQGQALRRLQEFAYWQARHAREGWRIRADLAECELTAPLDVDGETF
                     LLKGRIDRVDEHPDGSFLLLDYKTGDTARTPEETHGVRGDGEGEWTDLQLPLYRLLAA
                     SMGVAGNRVSLGYINLSKETEKDTLALASWDEACLSGAIETAREVIRRIRRQEFWPPG
                     AASSYGDAFSGICFDNCPNRAELLQNEPPVNAERRDG"
     gene            939..4112
                     /locus_tag="PLY56_07530"
     CDS             939..4112
                     /locus_tag="PLY56_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ21369.1"
                     /translation="MNTLIRASAGSGKTYALTTRYLRLLRAGASPQTILATTFTRKAA
                     GEILERVLKRLADAAAEEKALTDLRVALDSPELSAVECRRLLLHLCRNLHRVSISTLD
                     SFFHRLCRAFQLELGIPSGVTPVEQGSLADALLRRDALRATLTGNDLETMRDILNRLL
                     QGKAARSVVGVMSDVASQLYELYREAPAEAWQRLHVPPMPSPAELDAALAFLGAAASK
                     AKSKSMARAIEEDLARARAAKWAEFLDRGLAAKLVKGETTYSRQPIDPEVADAYEILF
                     QAAKATLLGELRARTEATYHLLSHFASHYDELRQRRGLMLFSDAPRALVGIASGCSPE
                     EIAYRLDAGVEHLLLDEFQDTSPEQWAILRPFAQACTASESRSFFCVGDVKQAIYGWR
                     GGSAEIFGRMEQDLAGLMAESLDVSYRSSQVVLDAVNAVFTGIANNPALQEYPKVAAT
                     WGAGYNAHKAHRDLPGWVELVTFPAASDTPEEGDGEGEATDPLDYVARRVREVHAAAP
                     QAEIGILMRTNAAVHAMLDALRREGVDASGEGPGRVDDDPAVELILSAMTLADHPGHS
                     AAAFHVAHSPLAAALDAPADLHVDAPCAARIARAIRRRLLVDGYGRVVADWARVLAEA
                     EYLDRRNTRRLLQLIEMADAFDAEASLRPGEFVQYVRKTSIEDAATSQVRVMTIHRSK
                     GLEFDAVFLPELHKRIVGQTPLVVIDRPSPTEPIRGVYRYPDKFHQLLLPELADVVAA
                     HRSREVSESLCVLYVAMTRARHALYLTVPPLKPLKSGKPASPGLSHAAILRYALVPDG
                     RPEGPAGGETLYAQGDPHWYTGLSAREEAAREAAAHHAPVRLKPVSGRPTRNYPRVTP
                     SSLGKDQRVPVEVLFGSPARGGQGALDSLEGARYGTRLHRLLALVSWSDEPLPDDETL
                     LRELRHALPDDTEAQRRRVLEQFHRLLAQPEVRGALARPKEASEVRLWRETPFSVLLD
                     DGIVEGRFDRVVVYRDAEGLLRADLLDYKSDTLSSTSLEAAAEAHRPQVAAYARALQK
                     MLGIPSTQVRAGLLFTAAGLCREVRVG"
     gene            4413..>4815
                     /gene="pyrR"
                     /locus_tag="PLY56_07535"
     CDS             4413..>4815
                     /gene="pyrR"
                     /locus_tag="PLY56_07535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOJ21370.1"
                     /translation="MDEQDIRRALTRIAHEIIEKNRGAEHIALVGIRTKGAPLAARLA
                     GLIQRIEGVPVPAGALDVATHRDDDRRAATDTSSIQFDITGRVIVLVDEVFYTGRTTR
                     AALDALMERGRPAAVQLAVLVDRGHRELPIRP"
BASE COUNT          780 a         1649 c         1560 g          826 t
ORIGIN      
        1 cttcttccgg ctgtaaagaa gagccgcttc tgggcgcctc gcccaaagcc ggccccagag
       61 ccgctgggcg aattggccgt gacggcgttt gccgcctatc tctcgtgccc ctatcgcttc
      121 tacctttctc gcatcctgaa gctgcgcgat gccgccgatg aagccgagga gatggacgcg
      181 ctcctcttcg gcagcctcgt tcatgacacg ctccgggcac tggcgactgg aacggcgcgc
      241 gattcgaccg atccctcaga gatcgccgcg ttcctcgaag cggatctgga gaggcgcatc
      301 tgcgagcagt ttgggggaga gccggcgcct gccatccgtc tgcaacaggg acaggccttg
      361 cgccgcctcc aggagttcgc ctattggcag gcgcggcacg ccagggaggg gtggcgtatc
      421 cgggccgacc tcgccgagtg cgagttgacc gcgccgctgg atgtggatgg tgagacattc
      481 ctcctcaagg gccggatcga ccgcgtagac gagcatcccg acgggagctt ccttctcctc
      541 gactacaaga ccggcgatac cgcgcgcacg ccggaagaga cgcatggagt ccgcggggat
      601 ggggaaggcg aatggaccga cctccagctc ccgctttacc gcctgctcgc cgccagtatg
      661 ggcgtggccg gcaaccgcgt ctcccttggg tatattaacc tgtccaagga gactgagaaa
      721 gatacattgg cgcttgcctc ctgggacgag gcctgtcttt cgggcgcaat cgagaccgct
      781 cgcgaggtga tccgccgcat tcgcaggcag gagttctggc cgccaggggc cgcatcttcc
      841 tatggcgatg ccttctccgg gatctgcttc gacaactgtc ccaatcgggc cgagctgctg
      901 cagaacgagc cgcccgtgaa tgctgagagg agagacggat gaataccctc atccgtgctt
      961 cggccggttc gggaaaaact tacgccctga ccacacgcta tctgcgcctc cttcgcgcgg
     1021 gcgcatcgcc ccagacgatt ctggcgacga ccttcacgcg caaggccgcg ggggagatcc
     1081 tggagcgtgt cctcaagcgt ctggccgatg ccgctgcgga ggagaaagcg ctcaccgacc
     1141 tgcgcgtggc cctcgatagc cccgagctca gtgccgtgga atgccggcgg cttctgctgc
     1201 acctctgccg caacctccac cgcgtttcca tctcgacact ggatagcttt ttccaccggc
     1261 tctgccgcgc ctttcagctg gagctgggca ttccatcggg agtcaccccg gtggagcagg
     1321 gcagcctcgc tgatgccctc ttgcgccgcg atgcccttcg cgctaccctc accggcaacg
     1381 acctcgaaac gatgcgcgat atcctcaacc gcttgcttca ggggaaagcg gcgcgctcgg
     1441 tcgttggggt gatgagcgac gtggcctcgc agctctacga gctgtatcgc gaagcccccg
     1501 ccgaggcgtg gcaacgcctc cacgtgcccc ctatgccctc gccagcggag ctcgacgcgg
     1561 cgctcgcctt cctcggggct gccgcgtcga aggcaaagag caagtcgatg gcccgcgcga
     1621 tcgaggagga tcttgcccgg gcacgcgcgg caaagtgggc cgagtttctc gaccgcggcc
     1681 tcgccgccaa gttggtgaag ggcgagacca cctacagccg gcagcccatc gatccagagg
     1741 tggccgatgc gtatgagata cttttccagg cggcgaaggc cacgctgctt ggcgagttga
     1801 gggcgcggac cgaggcgacc taccaccttc tgagtcactt cgccagccat tacgacgagc
     1861 tgcgccagcg ccgcggcttg atgctcttct ctgatgcccc ccgtgccctc gttgggatag
     1921 ccagcgggtg ctctccagaa gaaatcgcct accgccttga tgccggcgta gagcacctgc
     1981 tcctggatga gtttcaggat accagcccgg agcagtgggc gattctccga cctttcgctc
     2041 aggcatgcac cgcgagcgag tcgcgctcct tcttctgcgt tggcgacgtc aagcaggcaa
     2101 tttacggctg gcggggcggc agcgccgaga tcttcgggcg catggaacag gacctggccg
     2161 ggctcatggc cgagagcctc gatgtcagct accgctcgtc gcaggttgtc ctcgacgcgg
     2221 tgaacgccgt cttcacgggg atcgcgaaca acccagcgct ccaggagtac cccaaggtcg
     2281 ctgccacctg gggggccggc tacaacgccc acaaagcgca ccgcgacctc cccggctggg
     2341 tggagctggt gaccttccct gcggcgtcgg acacgcctga agaaggtgat ggcgagggag
     2401 aagccaccga tccgctggat tacgttgccc ggcgcgtgcg cgaggtgcac gcggccgcgc
     2461 cccaggccga gattggcatc ctgatgcgca cgaacgccgc ggtgcacgcc atgctcgatg
     2521 cgctgaggcg cgagggggtg gatgccagcg gcgagggccc cggacgcgtg gatgacgatc
     2581 ccgctgtgga gctgattcta tcggcgatga ctctggccga tcaccccggg cattccgccg
     2641 ccgcgttcca tgtggcccat tctcccctgg cggccgcgct tgacgcgccc gccgatctcc
     2701 acgtggatgc cccgtgcgcc gcccgcatcg cccgcgccat ccggcgccgc ctgctggtgg
     2761 acggctacgg ccgcgtcgtc gccgattggg cgcgcgtcct ggccgaggcg gaatacctcg
     2821 accggcggaa tacgcgacgg ctcctgcaac tcatcgagat ggcagatgcg ttcgatgccg
     2881 aagcgagcct gcgccccggc gagttcgtcc agtatgtgcg caagaccagc atcgaagacg
     2941 cggccacctc gcaggtgcgc gtgatgacga tccaccgctc gaaaggcttg gagttcgatg
     3001 ccgtttttct cccggagctg cacaagcgga tcgtcggtca gacgccgctg gtggtgatcg
     3061 accgcccatc gcctaccgag ccgatccggg gcgtctatcg ctatccagat aagttccacc
     3121 aactgctgct tccagagctg gctgatgtcg ttgccgcgca tcgatcgcgc gaggtcagcg
     3181 aaagcctctg cgttctctac gtggcaatga cgcgcgcccg ccatgccctc tatctgacgg
     3241 ttccgccgtt gaagccgctg aagagtggca agccggcgtc ccccgggctc tcgcacgcgg
     3301 cgatcctccg ctatgccctc gtgcccgacg ggcgaccgga ggggccggcg gggggcgaga
     3361 cgctctatgc ccagggagat cctcactggt ataccggtct ttcggcacgc gaggaggccg
     3421 cacgtgaggc tgccgcccat cacgcgccgg tgcgcttgaa accggtttcg ggccggccca
     3481 cgcgcaacta cccgcgagtt acgccgagct cgcttggcaa agatcagcgc gtgcccgtgg
     3541 aggtgctctt cggttccccc gcgcgcggtg gccaaggcgc cctggattca ctggagggcg
     3601 cccgctatgg cacgcgtcta caccgcctcc tcgctctcgt ctcctggtcc gacgaaccgc
     3661 tgccggacga cgagacgctc ctgcgcgagc tccgccatgc cctcccggat gacaccgagg
     3721 cgcagcgccg ccgtgtcctg gagcagttcc accgtctcct tgcccagccg gaagtgcgcg
     3781 gcgcgctggc gcgtcccaag gaagcgagcg aggtgcgcct ctggcgggag acgcccttca
     3841 gcgtgctcct cgacgatgga atcgtggagg gccgtttcga ccgcgtcgtg gtctaccgcg
     3901 atgccgaggg cctcctccgg gcggacctcc tcgactataa gagcgatacc ctctcatcca
     3961 cttccctgga ggcggccgcg gaggcccacc gcccccaggt ggcggcctac gctcgagcgt
     4021 tgcagaagat gcttgggatt ccatccacgc aggtgcgtgc cgggctcctc ttcaccgcgg
     4081 ctggcctgtg ccgcgaggtc cgcgtggggt aggggggccc agaggcacgg agaacccgga
     4141 atcctgccca cagaaacatg gaaaattccc tccttgacag agcgccgcgg atcgtgctag
     4201 actctcgtta acaagattgg gccgcaggca agcggctgga gacacccatc gcctttaagt
     4261 cggtcccgtg aggccgacaa ggtcggatgg tgagcactca tacctgggag cctccatgcg
     4321 ccagaaggcg aggaggctcc ctttcttatg taccgggttc ccgtgcaggg acctgaggag
     4381 atggcgtggc agcaacacat ggcacgcgcg tgatggatga gcaggatatc cgccgggcgc
     4441 tcacccgcat cgcgcacgag atcatcgaga agaaccgcgg cgccgaacac attgccctcg
     4501 tcggcatccg caccaagggc gcgcccctcg ctgcgcgcct ggccggattg atccagcgca
     4561 tcgagggcgt tcctgttcct gccggtgcgc tggatgtcgc cacgcaccgt gatgacgacc
     4621 ggcgcgccgc caccgacacc tccagtatcc agttcgacat caccggccgg gtcatcgtcc
     4681 tggtcgatga ggtcttctac accggccgca ccacgcgtgc tgctctcgat gccctgatgg
     4741 agcgcggccg acctgctgcg gtccagctgg ccgtcctggt cgaccgcggg caccgcgagt
     4801 tgccgatccg tccgg
//
LOCUS       DAOSCV010000252         4798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11175_length_4798_cov_2.851782, whole genome shotgun sequence.
ACCESSION   DAOSCV010000252 DAOSCV010000000
VERSION     DAOSCV010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11175_length_4798_cov_2.851782"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="PLY56_07540"
     CDS             <1..1064
                     /locus_tag="PLY56_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ21371.1"
                     /translation="VGWRVRFIVVIVRLSVILTYVAHPLVERGCRWRPRAIRHSSWRF
                     LVTLGVFLTLIALSVALMLWIWVPFQAQLNDLHQSIGDYQTAIEARLAGLREWYDRLP
                     ESMRAALDQQPDERVTHYLLGLVGGILARTVDWAARIWELILIPVLAFYFCLDSRSLK
                     RELLYLVPYRKVRETLFLLRQATRIMQSYVVAQLVLCLVAGVVVGGGLWILKVPYPLV
                     LGVLAGITRAVPIVGPVIGAIPITLLAMTVSLKMGMSVLLFFCILHLVESKVLLPELV
                     GHRMKLHPAIILIVLLVGAEFYGILGMFLAAPVAAILRMLLGFYLIEPEMRRRRALGG
                     MATGRLAPEEANDLFRKVS"
     gene            1096..2163
                     /locus_tag="PLY56_07545"
     CDS             1096..2163
                     /locus_tag="PLY56_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ21372.1"
                     /translation="MDPIAKRHGEGVEQETRQGWAARARQSLRHWLELPEEWHRLSPA
                     ARRRFVRESFQAYTFLLPALVIIGVFHLFAIFYAVYISLHDWSILKEAYIGLANYSKL
                     LADPEFRQSLLVTLWYVIGTVPVGLALSLGVAVLLFRPLRGVGFYRTLFFLPYITSLV
                     AAAAVWQWMYNPDYGVLNLALARLGLPTQRWLLEPRGVFALALEPLGIALPEWAAGPS
                     LALVSIIVMSIWSGLGFNVIVYLAGLGSIPRELQEAARVDGAGEWQVFRQVTMPLLSP
                     TTFFLVIIATIRAFQAFNQIYVMSPNDPTTRTLTMFIFQTFYGSSRAGYGAAAAMALF
                     VILLLLTIAQMRLGQEKVHYG"
     gene            2229..3167
                     /locus_tag="PLY56_07550"
     CDS             2229..3167
                     /locus_tag="PLY56_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ21373.1"
                     /translation="MAEEQRDKERAGTLAGAAREGERLAEAPGTTRPARSWARVRPGS
                     ALLIHAVLIVAGLAVAFPFYWMVITSLKTATETTSIPPTLFPKALTFSNYAEVWRAAP
                     FGRYFLNTVGIAGCVTLGVLFTSALAGHAFARMRFPGRQVLFVLLLATLMIPFEVLLI
                     PDYILITKLHWEDTYLAMIVPWMASVFGIFLMRQFFLGIPDELWDAARLDGCGHGTYL
                     SRIAVPLAGSGLAVVAIVSFLGSWNALLWPLVVVTRHPEIRPIQLGLAVFTTEFGVYF
                     HLVMAAATVTILPVLLLFLVAQRQFVESIARTGVKG"
     gene            3245..3997
                     /locus_tag="PLY56_07555"
     CDS             3245..3997
                     /locus_tag="PLY56_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21374.1"
                     /translation="MASGWSRDARVEGSQRRSRGVSGIARAGFLVMVAGILMTTGCAR
                     VPDEAAGPLVRRTLTVDFTVAGRINPLYYYYIALDTGGNPVEGPVPVVAFPWGNGWGT
                     GKITHFVLYHLGTFGVFRFVPGTDLLQADALGTPFDFNRPEDAPGNRIRVTLDVDATL
                     GQDVNVVNLNIIATDRINIEPTSLETKVVDALGFTGNQFLTLPLDIPQTFTNAQLTEP
                     EQGGDVPPALQPGVEEEDLDIIDWSAEVKINQ"
     gene            complement(3991..>4798)
                     /locus_tag="PLY56_07560"
     CDS             complement(3991..>4798)
                     /locus_tag="PLY56_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21375.1"
                     /translation="LISLNYSLMRKNGYTPFTSDYAPEAHQMSLSATFGSRIRRGVST
                     PAGYSGTYSSYSTGLTGGYGVGTSYAGAPYGWDNRRLDPAAAGKTYITFNTGYDFDLR
                     NASDLVVRTQYQPNAHHLIEMESAYDWFGKRRFGTQKRLRDIRARWYVDYGDPLQLAV
                     GTVWDTSRNRLGTIKASLSSQISPKWQVQSLYGRVASGYGSSQPYTQVMLTRDLHCWE
                     ASAIYTEGTFLGRKERELRFVMSIKAFPVNKEFGVSPSGQYLSTDIGSIY"
BASE COUNT          746 a         1503 c         1560 g          989 t
ORIGIN      
        1 acgtggggtg gcgggtacgc ttcatcgttg tcatcgtccg cctgtcagtc atcctcacct
       61 atgtcgccca tccgctcgtg gagcgcgggt gtcgttggcg tccgcgcgcc atacgccaca
      121 gctcatggcg ctttctggtt accttgggcg tcttcctcac gctgattgcg ctctcggttg
      181 ccttgatgct gtggatctgg gtaccattcc aggcacagct gaacgacctg caccagagca
      241 ttggcgacta tcagacagcg atcgaggcgc gccttgccgg tctgcgcgag tggtatgacc
      301 ggctcccgga gtcgatgcgt gcggcgctgg atcaacagcc ggatgagcgg gtcacccact
      361 acctcctcgg gctggtcggc gggattctgg cgcgaacggt agactgggca gcgcgtatct
      421 gggagctgat cctgatcccg gtgctcgcct tctacttctg cctggatagc cgcagtctga
      481 agcgagagtt gctctatctg gtcccctacc gcaaggtgcg tgaaacgctc tttctcctgc
      541 gccaggcgac gcgcatcatg cagagctacg tggtggcgca gctcgtcctc tgcctggtcg
      601 ctggcgttgt ggtgggaggg ggcctgtgga ttctgaaggt tccttacccg ctggtgctcg
      661 gggtgttggc agggatcacg cgtgcggtgc cgatcgtcgg ccctgtgatc ggcgcgatcc
      721 cgatcaccct cctggcgatg acggtctctc tcaagatggg gatgtcggtg ctcctcttct
      781 tctgcatcct gcatctggtg gagagcaagg tgctgcttcc tgagttggtt gggcaccgga
      841 tgaagctgca cccggcgatc atcctcatcg tgctgttggt aggcgccgag ttctacggca
      901 tcctcggcat gttcctggcg gctcccgttg ccgccatact tcggatgctc ctcggcttct
      961 acctgatcga gccggagatg cgccggcggc gtgccctggg cgggatggcc acggggcgcc
     1021 tcgccccgga agaggccaac gatctcttcc gcaaggtatc gtgaggcgcg agaggaagag
     1081 ggaggtttac gccgagtgga tccgatagcc aaacgccacg gggagggagt ggagcaggag
     1141 acgcgccagg gctgggccgc gcgcgcgcgc cagtcgctcc ggcactggct cgaattgccg
     1201 gaggagtggc accgcctctc tcccgcagcc cggcgccgct tcgttcgaga gagcttccag
     1261 gcctacacct ttctcctgcc cgcgctcgtg atcatcggcg tgttccatct tttcgccatc
     1321 ttctacgcgg tctatatcag cctccacgat tggtcgatcc tgaaagaggc gtacatcggc
     1381 ctggccaact actccaaact ccttgccgac ccggagttcc gccagtctct gctcgtcacc
     1441 ctctggtatg tcatcggcac cgtgccggtg gggctggcgc tctcgcttgg ggtggccgtg
     1501 ctcctcttcc gaccgctgcg cggcgtgggg ttctaccgga cgctcttctt cctgccgtat
     1561 atcacgtcac tggtcgccgc cgcggcggtt tggcaatgga tgtacaatcc ggattacggc
     1621 gtgctcaacc tggcgcttgc gcgccttggc ctgccaactc aacggtggtt gctggagcca
     1681 cggggcgtgt ttgcgctggc actggaaccg ctcggcatcg cgctcccgga gtgggccgcc
     1741 ggccccagtc tcgcgctcgt ctcgatcatc gtgatgagca tctggagcgg actcgggttc
     1801 aacgtcatcg tctacctggc cggcctgggg agcattcccc gcgagttgca ggaggcagcg
     1861 cgcgttgatg gggcaggcga gtggcaggtc ttccggcaag tcacgatgcc actcctctcg
     1921 ccaacgacct tcttcctggt aatcatcgcc acgatccgcg ccttccaggc gttcaaccag
     1981 atctatgtca tgtcgcccaa cgacccgacg acgcgcacgc tgacgatgtt catcttccag
     2041 acgttctacg gctccagccg cgcaggctat ggtgccgccg ccgcgatggc gctctttgtc
     2101 atcctgctcc tcctcaccat cgcgcagatg cgcctgggcc aggagaaggt gcactatgga
     2161 tgatcatcgg cgcgagggat gccgggagag atccttcccg caccatagga cgatacaggg
     2221 gatgctgcat ggctgaagag caacgagaca aagagagagc tggtacgctt gccggcgccg
     2281 cgcgcgaggg ggagcggttg gcggaggcgc caggaacaac acggccggcg cgctcctggg
     2341 cccgcgtgcg ccctggctcc gcgctgctca tccatgccgt cttgatcgtg gcggggctgg
     2401 ccgtggcgtt ccccttctat tggatggtga tcacgtcact caagacggca accgagacga
     2461 cgagcatccc cccgacgctt ttcccgaaag cgctcacctt ctccaactac gcggaggtgt
     2521 ggcgggcggc tcccttcggt cgctacttcc tgaacaccgt cggcattgcc ggctgcgtga
     2581 cgctgggggt gctgttcacc tcggccctcg ccggacacgc ctttgcccga atgcgcttcc
     2641 cgggccggca ggtgcttttt gtcctgctgc tggcgacctt aatgatcccc tttgaagtgc
     2701 tgctgatccc cgactacatc ctgatcacga agctgcactg ggaagacacc tacctggcga
     2761 tgatcgtgcc gtggatggcg agcgtcttcg gcatcttcct gatgcgccag ttcttcctgg
     2821 gcatcccgga cgagttgtgg gatgccgcgc gcctggatgg ctgcgggcac ggcacctatc
     2881 tgagccgcat cgcggtaccg ctcgcgggat cggggctggc ggtcgttgcc atcgtcagct
     2941 tcctcggctc atggaacgcg cttctctggc cgctggtggt agtgacacgc catcccgaga
     3001 tccggccgat tcagcttggg ctcgccgtgt tcaccaccga gtttggcgtc tacttccacc
     3061 tagtcatggc agccgccacg gtgacgatct tgccggtgct gttgctcttc ctcgttgccc
     3121 agcgccagtt cgtggagagc atcgcgcgca ctggcgtgaa gggatagaat agcgcgtcgg
     3181 ggagtggggg tttgtgccca ggcggttggg gaatgctatc atcgcttcgg taaggagatg
     3241 ggtcatggcc agtgggtgga gtagggatgc gcgcgtggag gggagccagc ggcggagccg
     3301 gggcgtatcc gggatcgctc gcgcgggctt cctggtgatg gtggccggca tcctaatgac
     3361 cacggggtgc gcgcgcgtgc ccgatgaggc ggcggggccg ctggtgcgcc ggacgctgac
     3421 agtcgatttc accgtcgccg gtcggatcaa tcccctctac tactactaca tcgctctgga
     3481 taccgggggc aatccggtgg agggaccggt gcccgtggtc gccttcccct gggggaatgg
     3541 ctggggcaca ggtaagatca cgcactttgt cctctatcac ctcggcacct ttggcgtctt
     3601 ccgatttgtg cccggcaccg acctcctgca ggcggatgcg ctcggcaccc cctttgattt
     3661 caaccgccca gaggatgccc cgggaaaccg cattcgcgtc actctggatg tcgatgcgac
     3721 actcggccag gacgtgaatg tggttaacct gaatatcatc gccacagacc ggatcaacat
     3781 cgagccgacc tcgctggaga cgaaggtggt ggatgctctc ggcttcacgg gcaaccaatt
     3841 cctgacgctg ccgctggata ttccgcagac cttcaccaac gcgcagctca cggagcccga
     3901 gcaagggggc gacgttcccc cggcactgca gccaggagtg gaggaggagg acctcgacat
     3961 catcgactgg agcgccgagg tcaagatcaa tcagtagatg ctgccgatat cggtcgagag
     4021 gtactggccg ctggggctaa ccccgaactc cttgttgaca gggaacgcct tgatgctcat
     4081 cacgaagcgg agttcccgtt ccttgcgccc gaggaaggta ccctcggtgt agatggcgct
     4141 tgcctcccag cagtgaaggt cgcgcgtcag catcacctgg gtgtagggct ggctggatcc
     4201 atagccgctc gcgactcgcc cgtagagcga ttggacctgc cacttgggag agatctgcga
     4261 gctgagcgat gctttgatgg tgcccaaccg gtttctggag gtgtcccaga cagtcccgac
     4321 ggccagctgc agcgggtccc catagtccac gtaccagcgc gcacggatat cgcgcaggcg
     4381 cttttgcgtg ccaaaccggc gcttgccgaa ccagtcgtac gcgctctcca tctcgatgag
     4441 gtgatgcgcg ttgggctggt actgcgttcg caccaccagg tcgctggcgt tgcgcaggtc
     4501 aaaatcatac cccgtgttga aggtgatgta ggtcttgccc gcagcggccg ggtcgaggcg
     4561 ccggttatcc cagccgtagg gcgcgcccgc gtaggaggtg ccgaccccat agccgccggt
     4621 gagcccggtg ctgtagctgg aataggttcc gctatatccc gctggcgtgc tgacgccacg
     4681 ccggatccgg ctgccaaacg tagccgatag cgacatctgg tgcgcctcgg gggcgtaatc
     4741 ggaagtgaac ggcgtgtatc cgttcttgcg catcagcgag tagttgagcg agatgagc
//
LOCUS       DAOSCV010000253         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11227_length_4780_cov_3.988360, whole genome shotgun sequence.
ACCESSION   DAOSCV010000253 DAOSCV010000000
VERSION     DAOSCV010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11227_length_4780_cov_3.988360"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLY56_07565"
     CDS             complement(<1..1080)
                     /locus_tag="PLY56_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21376.1"
                     /translation="MWWCVGVGLWALFPSQGMAQPVLEHQEVTEHHAPWDPGKRVVIR
                     HSYGAVRLRAVEEGTTVSVRAHHQLAGMTRQAIQAYLHAFRINVQAFDDRVEVVTIRP
                     TGEHKLLNQTTVDLDVVVPAEVLVDLSTRFGDLDVQGVGELKATAPFGAISVRSVAGN
                     VTLEGKHGKVQIHDVRGDVRVTHSLGDVEVTRVEGSLVARNQFAQIRAQGIRGRAVVE
                     NRSGPITLVNLSGGAEVTCQHAQVAVSRVTGDLQVTNRDESVAVADVQGTVVVVNANG
                     PVKARDINGDLKVDSRFGTVEVSQVRGKLTVEHSNGELSVRNVKGPAVLTNSHGLVQV
                     DGVEGDCRIRNEFGAVTVRMVLGRLD"
     gene            complement(1175..2185)
                     /locus_tag="PLY56_07570"
     CDS             complement(1175..2185)
                     /locus_tag="PLY56_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ21377.1"
                     /translation="MNCRYARKRLSEYIDGELSAVEHARIRAHLGECTECRAEEAALR
                     RTASLAQMWGVYQPGPELRWRLDSEYLAARARSLERLPWSARLQTFAPPAAVAMALLL
                     AVGLGHPFHSRRSASESSAPPRRVALAPPRPAPVVEAPAIEAPKPALPAEAQALPDEK
                     RVRETRAVPAKEPVQARPERPAPARRASRPAQIRSRSASQPSRIVARVQRRPSTASTA
                     QAPAQAAGKAPAKPEAGRVVTARLERVKLPEPLMRAEDLYASGRYLEAAGVLNAAIER
                     SSSQMPERVRSVYVERNALADATIAECSMALLHDPGNANARKFLDEAYESKVELLRSL
                     AG"
     gene            complement(2382..3827)
                     /gene="gltX"
                     /locus_tag="PLY56_07575"
     CDS             complement(2382..3827)
                     /gene="gltX"
                     /locus_tag="PLY56_07575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ21378.1"
                     /translation="MADQKEVRVRVAPSPTGDPHVGTAYIALFNYVFARHSGGKFILR
                     IEDTDRTRSTRESEEAILRALRWIGLEWDEGPDVGGPYGPYRQSERVEIYKEYAWELV
                     RKGRAYPCFCTAERLAELRAAQKQAKQDLGYDSLCRSIPPAEAEARVKAGECHVIRLA
                     VPDEGETIVPDRLRGELRFQNALIDDQILLKSDGFPTYHLANVVDDHLMKITHVIRAE
                     EWISSTPKHVLLYDAFGWEMPEWIHMPLLRNPDGSKISKRKQPVSIDYYRDIGILPEV
                     LLNFLALMGWSMGGDRERFTLDEMIAEFSFDKVTLGGPVFDLEKLSWLNGAAIRELSP
                     RELLERLKAWRLNDDFLLEVIPLVHERVRRLDEFIPATTFYFAGDLDYANVPELVPKN
                     RDARETAKLLEAALEIVDANPNLDKEILETRFRDLAAAHDWKPRELFMTLRVAVTGRT
                     VSPPLLESMQVIGREITRRRLRQAAAFLKGA"
     gene            complement(3941..>4780)
                     /locus_tag="PLY56_07580"
     CDS             complement(3941..>4780)
                     /locus_tag="PLY56_07580"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ21379.1"
                     /translation="AMQAARYAAELARATGGSVVLARAVSEAVARSRAYPARPPEDPV
                     LHEARDELRSAGVPIETRLIVARPGEAITNELKAEAYDLVTLGSRGKGESGSASLFLG
                     STALEVIRKARVPILVVRATEMGDPAGGEKPPRISTLLVPTDGSLASLSAADLGASLA
                     DALGAQATLLHVHPPRTPRKAGQAEAEILRTTAEAFTRRGVGVQTMAAAGDPSSAILR
                     VAREGPFDLILMGTYGAGEAWQYRMRMGSVAETVLTGAPCPVILVKHEVTMELDGGVR
                     RHE"
BASE COUNT          755 a         1695 c         1534 g          796 t
ORIGIN      
        1 atccagccgg cctagcacca tgcgcaccgt caccgccccg aactcattgc ggatccggca
       61 gtcgccttcc acgccatcca cctggaccag cccgtggcta ttcgtgagca cagcgggccc
      121 tttgacgttt cgcacgctca actcgccgtt gctgtgctcc accgtcagtt tcccgcgcac
      181 ctgcgacacc tccacggtgc caaaccggct gtccactttc agatcgccgt tgatgtcgcg
      241 cgccttcacc ggaccattcg cgttgaccac gaccaccgtg ccctgcacgt cggccaccgc
      301 gacgctctca tcgcggttgg tcacctgcag gtcgccagtc acgcggctca ccgccacctg
      361 ggcgtgctgg caggtcacct cggcgccgcc gctcaggttc accagggtaa tcggaccgct
      421 gcggttctcc accaccgctc gcccgcgaat cccctgcgcc cgaatctggg cgaattggtt
      481 gcgcgccacc aggctcccct cgacgcgcgt cacctccacg tcgccgaggg agtgcgtcac
      541 gcgcacgtcg ccgcgcacgt cgtggatctg aaccttgccg tgcttcccct ccagcgtgac
      601 gttcccggcg acgctgcgca ccgagatcgc cccgaaggga gcggtcgcct tcagctcgcc
      661 cacgccctgc acatccagat cgccgaagcg cgtggagaga tccaccagga cttccgccgg
      721 cacgacgaca tcgagatcca ccgtcgtctg gttgaggagc ttatgctccc cggtcgggcg
      781 gatcgtgacc acctcgacgc ggtcatcaaa cgcctgcacg ttgatccgga aggcgtgcag
      841 gtaggcctgt atcgcctggc gggtcatgcc agccagctgg tgatgcgcgc gcacgctgac
      901 agtggtgccc tcttccacgg cgcgcagacg taccgcccca taggaatggc ggatcaccac
      961 ccgcttgccg ggatcccagg gcgcgtggtg ctccgtcacc tcttgatgct cgagcaccgg
     1021 ctgggccatc ccctgcgagg gaaatagcgc ccacaggcca acgcctacgc accaccacac
     1081 gatcttccag gtagaaaaag ccctcatgca cgccacaccg cggcatcacg ccgccaggtc
     1141 ttgatcgccc cgcgccgttc tcccgcgctg cttatcagcc ggcgagcgag cgaaggagct
     1201 ccacttttga ttcgtatgcc tcgtccagga acttgcgcgc gttggcattt cccgggtcgt
     1261 gcagcagcgc catcgagcac tccgcgattg tggcatcagc cagggcgttc cgctccacgt
     1321 aaaccgagcg gacccgttcc ggcatctgag aggaagaacg ctcgatggcg gcgttcaaaa
     1381 cccctgcggc ctccaggtaa cgcccgctgg catacaggtc ctccgccctc atcaggggtt
     1441 cgggcagctt cacccgctcc aggcgagcgg tgaccacccg tccggcctcc ggcttcgcag
     1501 gagccttacc ggctgcctgt gccggggcct gcgccgtgct agccgtggag ggccggcgct
     1561 gtacgcgcgc cacaatgcgc gacggctggg aggcactcct gctccggatc tgtgccggcc
     1621 ggctggcgcg tcgcgctggc gccggccgct ccgggcgtgc ctgtacaggc tctttggccg
     1681 gcacggcccg cgtctctcgc actcgcttct catccgggag agcttgcgcc tcggcgggta
     1741 gcgccggctt gggcgcctca atggctggcg cctcgacgac cggtgcggga cggggcggcg
     1801 cgagggccac gcggcgaggc ggagcactac tctcgcttgc cgagcgtctg gaatggaagg
     1861 gatggcccaa gcctaccgcg agcagcagcg ccatggcaac cgccgctggc ggggcaaacg
     1921 tctggaggcg cgcgctccac ggcaatcgct cgaggcttcg ggcacgcgcc gcaaggtact
     1981 ccgaatccag acgccaccgg agttcggggc ccggctggta gacgccccac atctgggcca
     2041 gtgacgcagt gcgccgcagc gccgcctcct cagcacgaca ctcggtgcac tcccccaggt
     2101 gcgctcttat tcgcgcgtgc tcaaccgccg atagctcccc gtcgatatat tccgaaagcc
     2161 ttttcctcgc gtaacggcag ttcatgggcg ttcacccctc acggtcgttg ccgcttcttg
     2221 gactcggcag aacgccgaaa gtttcagcag accgcaagga tgcaagccac cgaaggcgtg
     2281 tggaggcggg ccggcccttc gccggtggcg tgggtggcat accccttcct gaccgacagg
     2341 gccggtccac tcgccgctca tcggtggttc gcgcgcatct cctacgcgcc cttgaggaag
     2401 gcggccgcct ggcgcagccg gcggcgggtg atctcgcggc caatcacctg catgctctcg
     2461 agaaggggcg gcgacaccgt gcgcccggtc acggccactc gcagcgtcat gaacaactcg
     2521 cgcggcttcc aatcgtgcgc agcggccaga tcgcggaagc gtgtctccag aatctccttg
     2581 tccaggttgg gattcgcgtc gacgatctcg agcgccgcct ccagcagctt cgccgtctct
     2641 cgggcgtcgc ggttcttcgg aacgagctcc ggcacgttgg cgtagtcgag atcccccgcg
     2701 aagtagaacg tcgttgccgg gatgaactca tccagccggc ggacccgctc gtggacgagg
     2761 gggatcacct ccagcaggaa gtcatcgttc agccgccagg ctttcaggcg ttccaggagc
     2821 tcgcgcgggg agagctcgcg gatagccgct ccgttcagcc acgagagctt ctccagatcg
     2881 aaaaccggtc ctcccagcgt caccttgtcg aacgagaact ccgcgatcat ctcgtccagg
     2941 gtgaagcgct cgcggtcgcc gcccatcgac cagcccatca gcgccaggaa gttgaggagc
     3001 acctcgggca ggatgccgat atcgcgatag tagtcgatgg agacgggctg cttccgctta
     3061 gagatcttac tcccatccgg gttgcgcagg aggggcatgt ggatccactc gggcatctcc
     3121 cacccgaagg cgtcgtagag gagcacgtgc ttcggcgtag aggagatcca ctcctcggcg
     3181 cggatgacgt gggtgatctt catgaggtga tcgtcgacca cgttcgccaa gtggtaggtg
     3241 ggaaagccgt cgcttttgag gaggatctgg tcgtcaatca gtgcgttctg gaagcgcagc
     3301 tcgccgcgga gccggtctgg cacaatcgtc tcgccctcgt cgggcacggc gagccggatg
     3361 acgtggcact cgccggcctt cacgcgcgcc tcggcctcgg cgggggggat ggagcggcaa
     3421 agcgagtcgt agcccaggtc ctgcttcgcc tgcttctgcg cggcgcgcag ctcggccagg
     3481 cgctccgcgg tgcagaagca ggggtaggca cggcccttgc gcacgagctc ccaggcatat
     3541 tccttgtaga tctcgacgcg ctccgactgc cggtaggggc cgtagggccc accgacgtcc
     3601 ggcccctcgt cccactccag gccgatccag cgcagcgcgc gcaggatcgc ctcctcgctc
     3661 tcgcgagtcg agcgcgtgcg gtcggtatcc tcaatccgca agatgaattt cccgcccgaa
     3721 tggcgggcaa agacgtagtt aaacagagcg atataggcgg tgccaacgtg gggatcgccc
     3781 gtcgggctgg gcgccacgcg cacgcggact tctttctgat cagccatcac ttcctccagg
     3841 ccccattata gcaggcgcct gggaatgagg caacgtagcg gcgcaaatct agaaggttgg
     3901 ggggatgcta ccagggcaac cgcgccgtcg ccgcccgaga tcactcgtgg cgcctgacgc
     3961 ccccatccag ctccatcgtg acctcatgct tcaccaggat caccgggcag ggggcgccgg
     4021 tgagcaccgt ctcggcgaca ctccccatgc gcatgcggta ttgccacgcc tcccccgcgc
     4081 cgtaggtgcc catcaggata aggtcgaagg ggccttcgcg cgccacccgc aggatagcgg
     4141 acgacgggtc tccggcggct gccatggtct ggactccgac gccccggcgt gtgaaggcct
     4201 cggcggttgt ccgcaggatc tccgcctcgg cctggccggc cttccggggc gtcctggggg
     4261 gatgcacgtg caggagagtt gcctgcgcgc ccagggcatc cgctagcgag gcaccgaggt
     4321 cggcggcgct cagggaggcc agcgagccat cggtcggcac gagaagcgta gagatgcgtg
     4381 gcggcttctc gccgccggcg ggatcaccca tctccgtggc gcgaacgacg aggatcggca
     4441 cccgcgcctt acggatcacc tccagggcgg tgcttcccag gaaaagactg gcgctcccac
     4501 tctcgccctt gccacggctg ccgagcgtga ccaggtcgta ggcctccgct ttcagttcgt
     4561 tcgtgatcgc ctcgccgggc cgggcgacga tcagtcgggt ttcgattgga acgcccgcgg
     4621 agcgaagctc gtcacgcgcc tcgtgcagca ccggatcctc gggcgggcga gccgggtagg
     4681 ctctggaacg tgccaccgct tccgacaccg cgcgcgcaag gaccaccgag ccgccggtcg
     4741 cccgcgccag ctccgccgca tagcgtgcgg cctgcatcgc
//
LOCUS       DAOSCV010000254         4777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11238_length_4777_cov_3.425455, whole genome shotgun sequence.
ACCESSION   DAOSCV010000254 DAOSCV010000000
VERSION     DAOSCV010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11238_length_4777_cov_3.425455"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="PLY56_07585"
     CDS             complement(<1..1783)
                     /locus_tag="PLY56_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ21380.1"
                     /translation="MWLTRLSITRPITILMLVGALLVIGLKSRSQLPVDLYPDIEFPA
                     VVIVTTYPGTGPEEIETLITKPIEDSVSSVNGIDKLSSTSSEGLSMVVIRFELGTDLD
                     TAVSDVRSKLDVTRGTLPDDADDPIVIKADTSAMPVLALSLSSNIRTPLELRDLAEDV
                     IKDRLSQVPGVASVAVSGGDVREIRVEVDRDRLEAHRLSITQVVAALQAENLNVPSGS
                     VDEARRTTAVRVLGEFQGPEEILDVRIPTATNPDLRIRDIAEVRDTVAEQTAYTRVNG
                     VPSVAIRVVKQSDGNTVEVVEGVRKELDRLTGGALSGKRQGGTKAAGPGGQQGPGGAS
                     LIPDDVQITIASDESTFIKDTIRDVNRSLLEGAFLAVVIVFLFLHMLRGTFIVALAIP
                     TSIIATFGVMHALGFSMNMMSMMGLALSVGILVDDSIVVLENIHRHLKMGAPPKEAAY
                     RGRSEIGLAAITITMVDVVVFFPIAFMGGMIGQFFRQFGVVVATATLFSLFISFTLTP
                     MLASRWLRPVSEEETGGQGLGARLAAAWDSAYGKLDRFYRGLLAWSLEHGSVAIAIGL
                     MTMVGSIATAMPKPAWPRLFAGLPAMAA"
     gene            complement(1793..3484)
                     /locus_tag="PLY56_07590"
     CDS             complement(1793..3484)
                     /locus_tag="PLY56_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ21381.1"
                     /translation="MWKRYVLVVAVVGVAGVGLSLRPGDKVRARPPAESAAGAAVEKD
                     AERRPAVSTMAVQASTIVHRVNVSGTLRAADEAVVTARSAGRVVWVIGKEGTPVRQGQ
                     VVIRLDDTDAQARVRSARAALEGARARLKQAKASATQQTTATDSGIRNAEAAVAAAEA
                     ALKQERTNAEAQEAALNAQVKAAEAALDAAQSQLALRRKGARDQERSMAEQSVRLAQA
                     TYDNAKTNYDRALKLFKTGAIAKAELDSAETQMRVSKAQLESAKEQLSLVETGSREEE
                     IQAAEAAVRQAEQGLAVARANLKQLDVARGRVTIAEANLRQAKAGLDAARANRQVNVV
                     RDQEVLAAETAVRQAQEALTLALQELDYMQIKSPVDGVVSKKQVEVGQSISPNVTLLQ
                     LAATDALYFEASVPELQATSLRAGQKVELRVDALAGGQASLYSDSKTQAIEGSVEKVV
                     PVVDQQTRSFTVRVTVPRHASLMPGMFARGSVIVARHEKAISVPRDVLVEKGDRRTVF
                     VVEDGVARARSVTTGVVDGNSVQITKGLAVGDQVVIVGQQTLADGDQVRVVTPGR"
     gene            3981..4205
                     /locus_tag="PLY56_07595"
     CDS             3981..4205
                     /locus_tag="PLY56_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21382.1"
                     /translation="MLSIILRTVELAILVGVVLLAKRQGWLVRRRRDAAGIAVLLLLW
                     LLLAWAVAAREQNQAGPTSPPPPVTALHTR"
     gene            4378..>4777
                     /locus_tag="PLY56_07600"
     CDS             4378..>4777
                     /locus_tag="PLY56_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ21383.1"
                     /translation="MSHYLTFDIGTTALKSALISSEGRLLALHTSEYTLRAPHPDRAE
                     MSPETYWRAAIEGARAVYVTAGAAPRALAAIGFSSQGQSFIPIDRAGRPLHDAIVWVD
                     SRAQAIAEEWEAAWLSREEYRRISGYAWIPA"
BASE COUNT          818 a         1670 c         1461 g          828 t
ORIGIN      
        1 cagcggccat ggccggcagg cccgcaaaca accgcggcca ggcgggcttg ggcatggcag
       61 tcgcgatact tcccaccatc gtcatcaatc cgatggcaat ggcaaccgac ccatgctcca
      121 gagaccaggc cagcaggcca cgatagaacc ggtccagctt tccataggca gagtcccacg
      181 ccgccgcaag gcgcgcgccg aggccctgcc cgccggtttc ctcctcgctc accggcctca
      241 gccagcgtga agcgagcatc ggggtgagcg tgaaggagat aaagagcgag aagagggtgg
      301 ccgttgccac cactacgccg aactgtcgga agaactgccc gatcatgccg cccataaagg
      361 cgatcgggaa gaacaccacc acgtccacca tcgtgatcgt gatagccgcc aggccaatct
      421 ccgaacgccc tcggtaggca gcctcttttg gcggggcccc catcttaagg tggcggtgga
      481 tattctccaa caccacgatc gagtcgtcca ccaggatgcc gacggagagg gcgaggccca
      541 tcatcgacat catgttcatg gagaagccga gggcgtgcat caccccgaac gtggcgatga
      601 tcgaggtggg aatcgccagc gcgacgatga acgttccgcg cagcatgtgc aggaagagga
      661 agacgatgac gaccgccagg aacgcccctt ccagcaacga gcggttcacg tcgcgaatgg
      721 tgtctttgat gaaggtcgac tcatccgagg cgatggtgat ctgcacgtcg tcgggaatca
      781 gcgaggcgcc tccgggcccc tgctggccac ccggccccgc tgcctttgtg cctccctggc
      841 gcttcccgga gagcgcgccc ccagtcagcc ggtctagctc cttgcggacg ccctcgacca
      901 cctccacggt gttgccatcg gactgcttga ccacccggat ggccacactg gggacgccat
      961 tcacgcgcgt gtaggcggtc tgttcggcca cggtatcgcg cacctcggcg atatcgcgta
     1021 tccgcaggtc gggattggtg gccgtgggaa tgcgcacatc caggatctcc tctggaccct
     1081 ggaactcgcc cagaacgcgc acggcggtcg tgcgccgcgc ctcgtcgacg ctaccggagg
     1141 gcacattcag attctcggcc tggagagcag ccaccacctg cgtgatgctg agccggtgcg
     1201 cttccaggcg atccctatct acttcgacac ggatctcgcg cacatcccca ccagagaccg
     1261 cgaccgatgc cacaccgggg acctggctga gacggtcctt gatcacatcc tctgccaggt
     1321 cgcgcagctc cagaggcgtg cgaatattgc tggagaggct gagcgcgagg accggcatcg
     1381 cgctcgtgtc cgccttgatg acaatcgggt cgtcggcatc gtctggcaag gtgccccggg
     1441 tcacatcaag cttcgagcgc acgtcggaga cagcggtgtc cagatcggta cccagctcga
     1501 accggatcac gaccatggag aggccctccg aggaggtcga ggaaagcttg tcgatcccgt
     1561 tgacggagct aaccgagtcc tcgattggct tggtgatgag cgtctctatc tcttccggtc
     1621 ccgtgcccgg ataggtggtc acgatgacga ccgccgggaa ctcaatatcg ggatagaggt
     1681 cgaccggcaa ttgcgagcgc gatttcaggc cgatcacaag cagcgccccc accagcatca
     1741 ggatggtgat cggacgcgtg atagaaagcc gagtgagcca catatgagtt tcctatctac
     1801 ccggcgtcac aacgcgaacc tggtcgccgt cggcgagggt ttgctgtcca acaatcacca
     1861 cctggtcgcc cacggcaaga cccttggtga tctgcaccga attaccatcc accactccgg
     1921 tggtcaccga acgcgcacgc gccacgccat cctccaccac gaacacggtc cggcgatcgc
     1981 ccttctcgac gagcacatcg cgcggcacgc tgatcgcctt ttcgtggcga gcgacgatca
     2041 cgctcccgcg ggcaaacatg cccggcatca gcgaggcatg ccgcggcaca gtcacccgaa
     2101 ccgtgaagct gcgcgtctgc tggtccacga cgggtaccac cttttcgacg cttccctcga
     2161 ttgcctgcgt cttggagtca gagtagaggc tcgcctgccc gcccgccagg gcatcgacgc
     2221 gcagttccac cttctgcccg gcgcgcagag acgtcgcctg aagctcggga acgctcgcct
     2281 cgaagtagag cgcatcggtg gcagcaagct gcaacagggt gacgttgggg gagatactct
     2341 ggccgacctc aacctgcttc ttggagacga cgccatccac cggcgacttg atctgcatgt
     2401 aatccagttc ctgaagcgcg agagtcaacg cttcctgggc ctggcgcacc gccgtctccg
     2461 cggcaagcac ttcctggtcg cgcaccacat tcacctgccg attggcgcga gccgcatcca
     2521 gccccgcctt ggcctggcgc aggttcgcct cggcaatcgt cacacggccc cgggccacat
     2581 caagttgctt caggttggcg cgggccaccg ccagaccctg ctccgcctgg cgcaccgcgg
     2641 cctcggcggc ctgtatctct tcctcgcgag agcccgtctc taccagtgag agctgctcct
     2701 tcgccgactc gagctgggct ttggagactc gcatctgcgt ctccgcgcta tccagctctg
     2761 ccttcgcgat ggcgcccgtc ttgaacagct tcagggcgcg gtcatagttg gtcttggcgt
     2821 tgtcgtacgt agcctgggcc agtctcaccg actgctcggc catggagcgc tcctggtccc
     2881 gggcgccctt gcgcctcagc gccagctggc tctgggcggc atccagcgcg gcttccgccg
     2941 ccttcacctg ggcgttgagc gcggcctcct gggcctcagc gttcgtacgc tcctgcttca
     3001 aggcagcttc agccgccgcc accgccgcct cggcgttgcg gatgcccgaa tccgtagccg
     3061 tcgtctgctg cgtggccgat gccttcgcct gcttcaggcg ggccctcgcg ccttcgagcg
     3121 ccgcgcgcgc agagcgcacc cgggcctggg cgtcggtatc atcgaggcga atcaccacct
     3181 gcccctggcg tactggcgtg ccttcttttc cgatcaccca gaccacccga cctgcactcc
     3241 gcgcggtgac caccgcctcg tccgccgcgc gcagcgttcc cgagacgttc acccggtgca
     3301 ctatcgtgct ggcctgcacc gccatcgttg aaacggccgg ccgtcgctcc gcgtccttct
     3361 caactgccgc tcctgcggcc gattcggcgg gaggccgcgc gcgcacctta tcacctgggc
     3421 gcaggctgag ccccacgccg gccactccaa ccactgcgac taccagaaca taccgcttcc
     3481 acatgcgacc ctccgctcca caagagggcc cgagaggccc ctctcgcacc tcatgagtac
     3541 gcgctcgctc gctccacgcc gcattcctct ggcacagcct caccccgcga gatgatcaac
     3601 ggattggagg tcagacgagc ggcacgcgct tgcagagttc ggatgccgtg tcggggctcg
     3661 ccgcgagttc tcgccatgcc aatgctggct gaatgagtga gcacgcactc aatatgatag
     3721 caatagcttt tccaggtgtc aaggagtttt cactcatacg aggccctgtt ttttcaacga
     3781 ttttgctggc cacgtgtgaa ccccactcaa tccctcggtg ggatccgtgg agagccgcgc
     3841 gcgcggcatc gtgccaaggc gacggggctg ggaggaaccg tgcctgcacg agccgaatcg
     3901 tgagcagggg ctgcccgagt gaaacccgcg ctctggcttc ggagcgtcgc tgccggcgcg
     3961 ggcaggaagg atgcgtgctt ttgctgtcga tcattctgcg cacggtcgag ctcgccatac
     4021 tcgtgggcgt cgtgctcctc gcgaaacgcc aggggtggct cgtgcgccgc cgccgcgatg
     4081 ccgcgggtat cgccgtgctc ctcctcctgt ggctgcttct cgcctgggcg gtcgcagcga
     4141 gagagcagaa tcaggcaggg cccacctccc cgccccctcc cgtgaccgct ttgcacacgc
     4201 gctgattgcg cagatggcga ggagagaggc gacatgagca tagagtggtt cccgcagccg
     4261 ggtgcaacga cacggagacc ccccgcccgg gagtagcgcg ggcctctcag caccgtgcgc
     4321 aacacccgcg ctctataccc gaacgcaacg tgcgcgaacg cacgcaggta cccggcgatg
     4381 tctcactacc tcaccttcga tatcggtacg acggcgctga agagcgcgct catctcctcc
     4441 gagggccgct tgctggccct ccacaccagc gaatacaccc tccgtgctcc ccatccggac
     4501 cgggcggaga tgagccccga gacctattgg cgtgcggcta tcgagggagc gcgcgccgtc
     4561 tatgttacgg ctggagcggc gccgcgcgcg ctcgcggcca tcggctttag ctcgcagggg
     4621 cagagcttca ttccgattga ccgtgccggc cgcccgctgc acgacgccat cgtctgggtg
     4681 gatagccgcg cccaggccat cgccgaggag tgggaggccg cctggctgtc gcgcgaggag
     4741 taccgccgga tcagcggtta tgcctggatc ccggcca
//
LOCUS       DAOSCV010000255         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11248_length_4775_cov_3.405932, whole genome shotgun sequence.
ACCESSION   DAOSCV010000255 DAOSCV010000000
VERSION     DAOSCV010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11248_length_4775_cov_3.405932"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLY56_07605"
     CDS             <1..1237
                     /locus_tag="PLY56_07605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ21384.1"
                     /translation="VSWQDTFLASRGGGTCRHRGQDILAPKMTPVVAAFDGTVRPRAR
                     NGHYLLTLVGDHGWSAEYMHLNNDTPGTDDGQGGELHAFAPGIYPGVRVVAGQLLGWV
                     GDSGNAEGTVPHLHFELVHQATGVAVNAAPSLRLARRIAAPRLPELLPDLKPGPGEVR
                     WNGFARAVDTGRGSIDLHVSARAERHEPLTALTRPQVCTVHCGEGTSLAVLGAGEARL
                     SLSEITPGAQLVVVGRKVDGEIVARAIYAEAAGLVPGKPLLAALAALVPAGPAVAATP
                     ALGPAGPAVATTPAVPSRREDPKRSNTAAAVAPVQSAEGLLWVHGYVLTDLSDGKMKI
                     RMDVVRLPGSLEMIRLRRALVKEVIVGTRTRLRDPRGKPIRPEALVANAEVEILGEEE
                     GKTAPLHAATLILRAAPRK"
     gene            1490..3394
                     /locus_tag="PLY56_07610"
     CDS             1490..3394
                     /locus_tag="PLY56_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ21385.1"
                     /translation="MQKATGRVSRRFAPVSFTDVAIEDRFWAPRIRVNREKTLAHQYA
                     QCKETGRIDAFRLDWKPGQEPVPHIYWDSDVAKWIEAASYSLATHPDRELDALLDEVV
                     SLIASAQQPDGYLNPHFTVVEPEKRWTNLRDCHELYCAGHLMEAAVAHFQATGKRTLL
                     DALCRYADYIGTVFGTEPGKKRGYCGHEEIELALVKLYHATGERRYLELACYFVDERG
                     RQPYYFDEEARARGEDPSRYRSGNYDYCQAHLPVREQTEVVGHAVRAMYLYSAMADLA
                     GETGDATLLAACERLWDNLTLRRMYLTGGIGPTRANEGFTCDYDLPNETAYAETCAAI
                     GLVFWAHRMLQLDCDSRYADVMERALYNGVISGVSLDGERFFYENPLASLGHHHRQPW
                     FGCACCPPNIARLLASLGQYVYSMGEGGVAVHLYIAGSARLRLNGALVTLRQETEYPW
                     TGRVVLRLEMEQPARFTLRLRIPGWCRGTAARVNGEAVDLSGKVVKGYACLEREWRNG
                     DRVELELPMPVERVYAHPEARQDIGRVALQRGPLVYCLEDVDNPVPIHRVMLPADAEL
                     TARFEEGVLGWEVMLAGHAVAVSDEGWESALYRTQPPVRVPVTIRAVPYCVWDNRAPG
                     RMAVWLPECA"
     gene            complement(3413..4531)
                     /locus_tag="PLY56_07615"
     CDS             complement(3413..4531)
                     /locus_tag="PLY56_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ21386.1"
                     /translation="MPEMTSRQRLLAAMRREQPDRVPIHVRGVPAWNEEWVRTRHPSY
                     APVIEAVARHGDLVATWSPPQGFFLSAAPLETETTRIPAGDWEIEQITVETPLGPLTR
                     ERHISRSGMPGLTTRFWIETLEDVERFLSLPYEPVKVDATPFFELERQLGERALVITG
                     LNNPATYTHMLLGSERLAIWSIEERALISRVMGLFAERVYDLVRALLEAGVRGVFGFT
                     GEEYIGPPLQSPRDFREWGTCLEQPIAELLHRYGCLMHIHCHGPMDAILEEFPAIGCD
                     CLHPIEAPPLGDVPLADAKRRIGDRVCLEGNVQIGDLYSLTPPEIRELVRHAIDDGAP
                     GGGFILCPTASPFTPTLEPKVVENYLAFIEAGVEWGRA"
BASE COUNT          782 a         1505 c         1717 g          771 t
ORIGIN      
        1 ggtttcgtgg caggacactt tcctcgcctc gcggggcggg ggcacgtgcc ggcaccgcgg
       61 ccaggatatt ctggccccga agatgacgcc ggtggtggcg gcgttcgatg ggaccgttcg
      121 cccgcgcgcc cgcaacgggc actacctcct cacgctggtt ggcgatcacg gctggtcggc
      181 tgagtacatg catctgaaca acgatacgcc cggaacggac gacggccaag ggggcgagct
      241 gcatgccttc gcgcccggca tctatccggg cgtccgggtg gtggccggcc agctcttggg
      301 gtgggtgggc gatagcggca acgccgaggg gacggtcccg caccttcatt tcgagctggt
      361 ccatcaggcg acgggcgtgg cggtgaacgc tgccccctcc ctgcgcctgg cgcgaaggat
      421 tgccgcgccc cggttgcccg aactgctccc ggatctgaag ccggggcctg gcgaggtccg
      481 ctggaacggc tttgcgcgcg ctgtcgacac cggacgggga agcatcgacc tgcacgtgag
      541 cgcgcgcgcg gagcgacacg aaccgttgac cgcgctgaca cgcccgcaag tatgcaccgt
      601 tcactgtggg gaaggcacgt cgctcgcggt tctaggggca ggcgaggcac gcctctccct
      661 gtcggagatc acacctggcg cgcagctggt cgtagttggc cgaaaagtgg acggtgagat
      721 cgtggcccgc gccatttacg ccgaggcagc cggtctcgtg cccggcaagc ccctcctcgc
      781 cgctctcgcg gcgcttgtgc cggccgggcc ggcggttgct gccaccccgg cgctcgggcc
      841 ggccgggccg gcggttgcta ccacccccgc tgtcccctcg cgccgggaag atccaaagag
      901 gtcgaacacg gcggctgcgg tcgcgccggt gcaatctgcg gagggcctcc tatgggtgca
      961 tggctacgtc ctcacggatc tgagcgatgg gaaaatgaag attcgtatgg atgtggtgcg
     1021 cctgcccgga agcctggaga tgatccggct ccgtcgcgcc ctggtgaagg aggtcatcgt
     1081 tggcacccga acccgcttgc gtgaccccag gggcaagccg attcgcccgg aggcgttggt
     1141 cgcgaatgcc gaagtggaga tcctgggcga ggaggagggg aagacggctc cactgcacgc
     1201 cgcgacgctc atcctgcggg ccgcgccacg gaagtaggcg ggaagcgccg gctgccctcc
     1261 cttgaagctt tgggaaggag caaaggggca agccaggtgc gggaattggg cgcatgctcg
     1321 ccgaactctg gcacagggcg atggtctctt gccggcacgc ttctcttggg cctggacaaa
     1381 ggagcatccc attcaggaca cggctggtga ggagcgagcg acccgcctca taggtggaca
     1441 tgacgcgccc gataggaacc actcgggctg gcgtaaagga gagttatcga tgcagaaggc
     1501 gaccggtcgg gtgagccggc ggttcgcgcc ggtgtcgttt acggatgtcg cgatagagga
     1561 ccgcttttgg gcgcctcgga tccgggtgaa ccgcgagaaa acgctcgcgc accagtacgc
     1621 gcaatgcaag gagacgggcc ggatcgatgc cttccggctc gattggaagc cgggccagga
     1681 gccggtgccc cacatctact gggactccga cgtggccaag tggatcgagg cggcgagcta
     1741 ctccctcgct acccatccag accgggagct ggacgcgctc cttgatgagg tggtgagcct
     1801 gatcgcttcc gcgcagcaac cggatggcta cctgaacccg cacttcactg tggtggagcc
     1861 ggagaagcgg tggacgaacc tgcgcgactg tcacgagctc tactgcgccg gccacttgat
     1921 ggaggcggca gtcgcccatt tccaggcgac ggggaagcgc acgctgctcg acgccctctg
     1981 ccgttacgcc gattacatcg gcaccgtctt cggcaccgag cctgggaaga agcggggcta
     2041 ctgcgggcac gaggagatcg agctagcgct agtgaagctc taccacgcca cgggcgagcg
     2101 ccgctatctg gagctggcct gctacttcgt ggatgagcgt gggcggcagc cctactactt
     2161 cgatgaggag gcaagggccc ggggcgagga cccctcgcgc tatcgctccg gcaactacga
     2221 ctactgccag gcgcacctgc cggtgcgcga gcagaccgag gtggtgggcc acgccgtgcg
     2281 cgcgatgtac ctctacagcg ccatggcgga cctggcgggc gagacgggcg atgccacgct
     2341 cctggccgcg tgcgagcgcc tctgggataa tctgacgctg cgccggatgt acctgaccgg
     2401 cggaatcgga ccgacgcgcg cgaacgaagg cttcacctgc gattatgacc tgcccaacga
     2461 gaccgcctac gccgagacgt gcgcggcgat tggcctcgtc ttctgggcac accggatgct
     2521 ccagctcgat tgcgactcgc gttatgccga cgtgatggag cgggcgctct ataacggcgt
     2581 gatcagcggc gtctcgctcg atggcgagcg attcttctat gaaaaccccc ttgccagcct
     2641 ggggcatcac caccgccagc cgtggtttgg gtgcgcgtgt tgcccgccca acatcgcccg
     2701 cctcctcgcc tcgttgggac agtatgtcta ctccatgggc gaaggcggcg tggccgtgca
     2761 cctctacatc gccgggagcg cgcgcctgcg attgaatggt gcgctggtca cgctccgaca
     2821 ggagacggag tacccctgga ctgggcgcgt ggtgctccgc ctggagatgg agcagccggc
     2881 gcggttcaca ttgcgcctcc gcattcccgg atggtgccgg ggcacggcgg cgcgcgtcaa
     2941 cggcgaggcg gtcgaccttt caggcaaagt ggtgaaaggc tatgcctgcc tggagcgcga
     3001 gtggcgaaac ggcgaccgtg tcgagctgga gctaccgatg ccggtggagc gcgtctacgc
     3061 ccatcccgag gcgcgccagg atatcgggcg cgtggcgctg cagcgcggtc ccctcgtcta
     3121 ctgcctggag gacgtggata accccgtccc gatacaccgg gtgatgctgc cggccgatgc
     3181 cgagttgacc gcccgcttcg aggaaggcgt gttgggctgg gaggtgatgc tcgctggcca
     3241 tgccgtagcg gtctcggatg agggatggga gagcgccctc taccggaccc aacccccggt
     3301 gcgcgtgccg gtcaccatcc gcgccgtgcc gtattgcgtt tgggacaacc gtgccccagg
     3361 gcgcatggcc gtctggcttc ccgagtgcgc gtgagtcgtt cgggcatgcg gctcacgccc
     3421 tgccccactc cactccggcc tcgatgaacg ccaggtagtt ctcgaccacc ttaggttcca
     3481 gggtgggggt gaaaggcgag gcagtggggc acaggatgaa gccgccgccc ggcgcgccat
     3541 cgtcgatcgc gtgccggacc agctcccgaa tctcaggcgg agtgagcgag tagaggtcac
     3601 cgatctggac gttcccctcc agacaaacgc ggtcgccgat gcggcgtttc gcgtccgcga
     3661 ggggcacatc gccgagggga ggcgcttcga ttgggtggag gcaatcgcac ccgatggcag
     3721 ggaactcctc caggatggca tccatcgggc cgtggcagtg gatgtgcatc aggcagccat
     3781 accggtgcag gagctccgcg atcggctgct ccaggcacgt gccccactcc cggaagtctc
     3841 ggggcgactg gagcggcggg ccgatatact cctctcccgt gaagccgaaa acgccgcgga
     3901 cgccggcttc caggagggcg cgcaccaggt cgtacacgcg ctcggcgaag aggcccatca
     3961 cccgggagat gagcgcgcgc tcctcaatgc tccagattgc cagccgctcg gatcccagaa
     4021 gcatgtgcgt ataggtggcg gggttgttca ggccggtgat gacgagagcg cgctcgccaa
     4081 gctgacgttc cagctcgaag aagggggttg catccacctt cacgggctcg tagggaagcg
     4141 agaggaagcg ctcgacatct tcgagcgttt cgatccagaa gcgcgtggta agcccgggca
     4201 tccccgagcg gctgatatgg cgctcgcggg tgagcgggcc caggggcgtc tccacggtga
     4261 tctgctcgat ctcccaatcg cccgcgggaa tgcgcgtcgt ttcggtctcc agaggggccg
     4321 cggagaggaa gaagccctgc gggggcgacc aggtggcgac gagatcgcca tgccttgcga
     4381 ccgcctcgat aacgggggca taggaggggt ggcgggtgcg cacccactcc tcgttccagg
     4441 cgggcacgcc gcggacgtgg atgggaacgc ggtcgggctg ttcgcggcgc atcgcggcga
     4501 gcagccgctg gcgcgatgtc atctctggca tggcctgagc ctccgcggag aagttcgctg
     4561 gcgatgggcg tgcctccttg ctcggggtgc tgggaatgcc catgcaggcc gggaccggcg
     4621 gtccaggaga gttggtcgag agcgagcagt tcgcgcgggg ctgcccgtgc gaggcattgc
     4681 tgatgccggc agatggtcaa gataggagag agaagatgcc cgaggagcga gcgtatttgc
     4741 gcgaattggc ccggcgcgtg gcggagatcg ccgcg
//
LOCUS       DAOSCV010000256         4763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11283_length_4763_cov_2.745115, whole genome shotgun sequence.
ACCESSION   DAOSCV010000256 DAOSCV010000000
VERSION     DAOSCV010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11283_length_4763_cov_2.745115"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1796
                     /locus_tag="PLY56_07620"
     CDS             <1..1796
                     /locus_tag="PLY56_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOJ21387.1"
                     /translation="EHPVGRCFRYSDIGFILLGEVVRRVSGLRLDEFTQRHLFAPLGM
                     SETCFCPPASWAARCAATELRDGVSLRGQVHDENAAALGGVAGHAGLFSTASDLARFC
                     QMLLNDGELEGTRVLSPLSVRAMLAPQSTCLGSARGFGFDLHSAYASIRGDLLPSGSA
                     GHSGFTGTSLWIDRDLGLAIILLTNVVHPTRERSARRLRSRVSNVVAAALKPPSATVP
                     DRVRDPLLVRLGVDLLREEGCRRLAGEAVGLITNHTGRCADGTPTLRMLLEHGVKVRA
                     LFSPEHGIAGRLDEEVASGRDEETGLPIHSLFGETRSPLPEWLEGLETLVFDIQDIGA
                     RFYTYISTMGLCMEAAAKHGLRFVVLDRPNPITGGHVEGPILDLRLRNFAGYYAIPIR
                     HGLTVGELARLINVEIGCHLEVVPVQGWRRSMWWDQTGLEWVNPSPAMRTLNAATLYP
                     GLCCLESANLSMGRGTDTPFEVIGAPWIEPRRLAAELTARDIPGIGFVPVHFTPQLRD
                     YQGERCGGLYCVVTDREAFQPVRMVIHLAELLHRFHTREFTFDRIDTLVGTERVRVLL
                     EQFAPAEAIIAEWEEDEAGFRERRAPFLLYE"
     regulatory      2134..2242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2323..3651
                     /gene="thiC"
                     /locus_tag="PLY56_07625"
     CDS             2323..3651
                     /gene="thiC"
                     /locus_tag="PLY56_07625"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOJ21388.1"
                     /translation="MREQWVASRRGENVTQMHYARRGEVTEEMRFVAEREQVSPEFVR
                     AEVARGRAIIPANIRHTRLEPMAIGTNFRCKVNANLGNSSTTSDIEGELEKLRIALEC
                     GADTVMDLSTGGDIAVIREAILAASPVPVGTVPVYEALARVEAPEELTPALLLETIEA
                     QARQGVDYMTIHAGLLQEYLPLAARRVTGIVSRGGAILAQWMVAHNAQNPFYTHFEEI
                     CEIFRTYDVCFSLGDGLRPGCLADANDEAQFAELATLGELTERAWKHDVQVMVEGPGH
                     VPFDRIEENIRKQRELCHEAPFYLLGPLVTDIAPGYDHITSAIGGTMAAFCGAAMLCY
                     VTPKEHLGLPNPEDVRAGMVAHKIAAHAADIARGRPGARQRDDEMARARFGFNWERQF
                     ELALDPDTARAMHDETLPEPCHKSAHYCSMCGPRFCAMRMTHQIKERVRE"
     gene            3688..>4763
                     /locus_tag="PLY56_07630"
     CDS             3688..>4763
                     /locus_tag="PLY56_07630"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21389.1"
                     /translation="MWYNRAMHPTLAILAAKRQSVRNKLRPLPFELAVSLPLLAVMVA
                     VFLLGGYRFFFILFRFLRDSPPFGTMLTERLLAMVFLAFLSMLVFSNIVTSLSTLYLA
                     GELEFLLSRPIPYAVTFFAKFWESVLYSSTAFVVMGLPIFLAYGRVHEAPLYFYPLVF
                     LFYLPFLIAPAAAGTVITMLLARIFPARKTRATFCALFVALLAGLAVAAHLLGKGTFA
                     QVEAGVTMSQLMDALRLSDIPYLPNHWISQGIIASAQGDLRTAGFYFLALLSTALLCL
                     ELCSWLAPRVYYPGWARTRESDTRRSGRVVLPIFTWLEWLMRPIPRPVRALLLKDLRT
                     FWRDPAQWAQLLLLFGLLIIYISNL"
BASE COUNT          713 a         1631 c         1527 g          892 t
ORIGIN      
        1 tcgaacaccc ggtgggccgt tgcttccggt acagcgatat cggcttcatc ctcctcggag
       61 aggtggtccg gcgcgtctct ggcctgcggc tggatgagtt cacccaacgc cacctcttcg
      121 cgcccctggg catgagcgaa acctgctttt gcccgccggc atcctgggcc gcgcggtgtg
      181 ccgcgacgga gcttcgcgac ggcgtttccc tccgtggcca ggtccatgat gagaacgcgg
      241 cggcgctggg cggcgtcgcc gggcatgccg ggctcttctc caccgcctcg gacctagccc
      301 ggttctgcca gatgctgctc aacgacggcg agttggaggg cacgcgtgtg ctcagcccgc
      361 tcagcgtgcg cgcgatgctg gcaccgcagt ccacctgcct gggaagcgca cgcggtttcg
      421 ggttcgatct ccactcggcc tatgcatcga tccgcggcga cctgctgccg tcgggatccg
      481 ccgggcatag cggcttcacc ggcacgtctc tctggatcga ccgcgatctg gggctggcca
      541 ttatcctcct gaccaacgtg gtgcatccca cgcgggagcg ctccgctcgc cggctgcgct
      601 cgcgcgtcag caacgtggtg gccgccgcgc tcaaaccgcc ctcggcaacg gtgccggacc
      661 gcgtgcgcga tccgctcctg gtgcgccttg gggtggatct ccttcgcgag gagggatgcc
      721 ggcggctggc cggagaggcc gtgggcctca tcaccaacca caccgggcgc tgcgccgacg
      781 gcacgcctac cttgcggatg cttctggagc atggcgtgaa ggtccgggcg ctcttcagtc
      841 ccgagcacgg aatcgccggc cgcctggatg aagaagttgc atccggccgg gatgaagaga
      901 cgggcctgcc gatccacagc ctttttggcg agacgaggag ccccctgcct gaatggctgg
      961 agggcctgga gaccctcgtc ttcgacatcc aggatattgg cgcgcgcttc tacacctaca
     1021 tcagcacgat gggcctgtgc atggaggccg cggcaaagca tggactgcgc tttgtcgtgc
     1081 tcgaccggcc caacccgatc accggtggcc acgtcgaggg tcccatcctg gatctgcgcc
     1141 tgcgcaactt cgccggctac tacgcgatcc cgatccgcca cggcctcacc gtgggcgagc
     1201 tagctcggct gatcaacgtg gagatcgggt gtcacctgga ggtggtgccg gttcaggggt
     1261 ggcggcgctc gatgtggtgg gatcagaccg ggctggagtg ggtgaacccc tcgccggcaa
     1321 tgcgcacgct gaacgccgcc acgctctacc ccggactttg ctgcctggag agcgccaacc
     1381 tctcgatggg ccgtggcacc gacacacctt tcgaggtgat tggcgccccc tggatcgagc
     1441 cgcggcgcct tgccgcggag ctcaccgcac gcgacatccc cggaatcggc ttcgtaccgg
     1501 tccacttcac tccccagctg cgtgactatc agggcgagcg ctgcggcggc ctctattgcg
     1561 tggtcaccga ccgcgaggcg ttccagcctg tccgaatggt cattcacctg gcggagctgc
     1621 tccaccgctt ccacacgcgc gagtttacct tcgaccgcat cgacaccctc gtcggcacag
     1681 agcgcgttcg cgttctcctc gagcagttcg cccccgcaga ggcgatcatc gccgagtggg
     1741 aggaagacga ggccggcttc cgcgagcgcc gcgccccctt cctcctctac gagtagggct
     1801 caccagcgga agcgcaaacc ccacgggatt gcgcggggga tctgggattc cggcatatgg
     1861 agagggccgc ccgcacgcgg agctcgaagc tccgcgctgc tttccggcaa agccccttcg
     1921 gtgcttcccc tcccagccac cgccgttttg aggtatccgg gtgtatcccc agtccagccc
     1981 ggcagggctt cgctcgcaag cagcgcggtg atttatcgcc gcgcgccgga ttgcgcgctg
     2041 cctccgaggc ttcggattcc ttatgtcgga atctcagctt cctccgggtt tggcttgaca
     2101 cccgcgtcgg gcgcggggta taatcggctt gagaacactc gctgggggtg ggcccgtccc
     2161 tgagaaccgc ctgcgtggag gcggtaaccc ctggaacctg atccgggtaa tgccggcgta
     2221 gggaaagcga ccgcgacgcc atcatcgcga tactcttcgc gccgtccagc cgccttccgc
     2281 ttcgccggaa gcgcggctgt ggttgttttt tggagcgtaa agatgcgaga gcagtgggtg
     2341 gcaagccggc ggggcgagaa cgtgacgcag atgcactatg cccgcagggg cgaagtgacc
     2401 gaggagatgc gctttgttgc cgagcgcgag caggtctcgc cggagtttgt ccgggcagag
     2461 gtggcccgcg gacgcgccat catccccgcg aacatccggc acactcgcct ggagccgatg
     2521 gcgattggca cgaacttccg gtgcaaagtc aacgccaacc tgggcaactc atccaccacc
     2581 tcggatatcg aaggagagct cgagaagcta cgcatcgccc tggagtgcgg cgccgatacg
     2641 gtgatggacc tgagcaccgg cggcgacatt gcggtgatcc gcgaggcgat cctggcggcc
     2701 agtcccgttc cggtgggcac ggtgccggtc tacgaggcgc ttgcccgtgt cgaggcgccg
     2761 gaagagctga cgccggcgct attgctggag accatcgagg cgcaggcgag gcagggcgtg
     2821 gactacatga cgattcacgc gggcctgctc caggagtatc tccccctggc ggcgcggcgc
     2881 gtcaccggca tcgtgagccg tggcggcgcc atcctggctc agtggatggt cgcccacaac
     2941 gcgcagaacc ccttctacac gcactttgag gagatctgcg agatcttccg gacctatgac
     3001 gtctgcttca gcctgggcga tggccttcgg cccggatgcc tggccgacgc gaatgacgag
     3061 gcgcagttcg cggagctagc cacattgggc gagctgaccg agcgcgcctg gaagcatgat
     3121 gtccaggtga tggtcgaggg tccggggcac gtgccgttcg accgcatcga ggagaatatc
     3181 cgcaagcagc gagagctctg ccacgaggcg cccttctacc ttctcggccc gctcgtcacc
     3241 gatatcgctc ccggctatga ccacatcacc agcgccattg gaggcacgat ggccgccttt
     3301 tgtggcgcgg cgatgctctg ctacgtcacg cccaaggagc acctgggcct gcccaacccg
     3361 gaggacgtgc gcgccggcat ggtggcgcat aagatcgccg cgcacgcggc cgatatcgcc
     3421 cgcggccgac cgggcgcgcg ccagcgcgac gacgaaatgg cccgcgctcg cttcggtttc
     3481 aattgggaac gccagttcga attggccctc gatcccgaca ctgcgcgtgc catgcacgac
     3541 gagacgctcc ccgagccatg ccacaagtcc gcgcactact gctccatgtg cgggccgcgg
     3601 ttttgcgcca tgcgcatgac gcaccagatc aaagagcggg ttcgcgaata gcgcggaaag
     3661 tgatggggcg gcgcgcgggg cggccggttg tggtataata gggccatgca tccgacgctc
     3721 gccatcctgg ctgccaagag gcaatcggtc cgcaacaagc tccgccctct cccgttcgag
     3781 ctggcggtga gcctgcctct gctggcggtg atggtggcgg ttttcctcct cgggggctac
     3841 cgcttcttct tcatcctgtt ccgcttcctt cgcgatagcc cgccgtttgg cacgatgctc
     3901 accgagcgcc tgctggcgat ggtcttcctc gccttcctct cgatgctggt attcagcaat
     3961 attgtcacct cgctcagcac gctctacctg gccggcgagt tggagtttct cctctcgcgg
     4021 cccattccgt acgccgtcac tttcttcgcc aagttctggg agtcggtgct ctatagctcc
     4081 accgccttcg tcgtgatggg gctgccgatc ttcctcgcgt atggccgtgt ccatgaggcg
     4141 cccctctact tctatccgct cgtttttctc ttctatctcc cctttctgat cgcgcccgcg
     4201 gcagccggca ccgtgatcac aatgctcctg gcgcggatct tcccggcgcg caagacccgc
     4261 gccaccttct gcgccctctt tgtcgccctt ctggccgggc tcgccgtcgc ggcgcacctg
     4321 ctggggaaag gcaccttcgc gcaggtggaa gcgggcgtca cgatgagcca gttgatggat
     4381 gccctccggc tttcggatat cccctacctt ccaaaccact ggatctcgca ggggattatc
     4441 gcctcggccc agggcgacct gcgcacggcc ggcttctact tcctggcgct cctcagcacg
     4501 gcgctcctct gcctggagct ctgctcgtgg ctcgctccga gggtctatta ccctggatgg
     4561 gcgcgaacac gcgagtcgga cacgcggcgc tccggccgcg tcgtgcttcc gatcttcacc
     4621 tggctcgagt ggctgatgcg cccgataccc cgccccgtgc gcgcgctcct gctgaaggat
     4681 ctgcgcacgt tttggcgcga cccggcgcag tgggcgcagc tcctgcttct cttcggcctg
     4741 ctgatcatct acatcagcaa cct
//
LOCUS       DAOSCV010000257         4751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11332_length_4751_cov_2.843271, whole genome shotgun sequence.
ACCESSION   DAOSCV010000257 DAOSCV010000000
VERSION     DAOSCV010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11332_length_4751_cov_2.843271"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..949
                     /locus_tag="PLY56_07635"
     CDS             158..949
                     /locus_tag="PLY56_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21390.1"
                     /translation="MKTKQHGFTLIELLTVIAIIAILAAILLPVFGRAREQGRRTSCM
                     SNLHQIALAMKMYKQDCRGYPLDWPETVAGHRPFYTGSRWYGNDSKTQPGYGIATLYP
                     DYVPNLRVFNCPNNDETNPKFSNGVDGKDYAHWIGDGDENNYRHFNSYDGMDPLFVIS
                     GDEELTAHGSAYKYRRRPWATDAYTGGSAVISRDQAKRMLIWRNPSEDTVITWCSQHR
                     ADPYSGTPRSGDLDLIVYLDGSTKTVKSNPGAGESGHGSNAAGAQ"
     gene            1037..1843
                     /locus_tag="PLY56_07640"
     CDS             1037..1843
                     /locus_tag="PLY56_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ21391.1"
                     /translation="MRTSRAGFTLVEILTVIAIIVILAAVLLPVFGRAKEQSNRTHCL
                     SNLHQIATAIKLYKLDERGYPYDLQETVASPSPRPWIPGERWFGSGTDADGDGLVDTP
                     DKQHPGYGLATLYPHYIENIRVFNCPNNETDVLKDSEMEDPPDPPITEAQAEALTRWS
                     FQSYDGWDTALNQLKYDRYWRNEPGPSDPPDRHYIRQLRWRYPPEDTVLTWCTFHRAV
                     IDPTDPQLENLRKTDRDLVVYLDGTTRVVPSWAEVAEQGSGHLSTPGEQE"
     gene            2256..4184
                     /locus_tag="PLY56_07645"
     CDS             2256..4184
                     /locus_tag="PLY56_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21392.1"
                     /translation="MRAFESDITGKVLAGITRPGATRVGERGTTLLEVLVVSLVLLVG
                     ILTVIRVFPTGFDVVERGAYVTMANRYAKTLLDDLCSRPEALPDGVLPAVYLSTEYQG
                     TRWWINDDQVQTSDLEPRPPGTGDGGLRAWANSLVQFRRIHQETTRVPRAAYRDTDGS
                     LLARYALRFAPIEFYNSETPGYYDLMTVYSLRPYVKVDRDILERYAGRDGYYAIDPDE
                     EDEQHAGAPRQIYFSRVSYRRTFKIDFAVRPVNVNPNDYNPDDVEYVTDRIVWVDADS
                     DVAEDAAGVVNLGDGVSTVLVPESEVVLRRLRDARAEPGSGDPLWYRLINATVGLLEF
                     GPGTAGLPVGINYSVMDWQILHDDVDVPMEPDPGQTAPAEEQLAWVKLSFPFIERNPI
                     GAPEDDRPGMQEAIILQNIDPNSPNYGEVIDPNNDNNGADDYPTWTFDDPQRATDNSL
                     AGRLGLRVAKFNQLGVRRVRVFYRTRDGFAAMVVKPYARYTPVPAAARVPSYNRYRSS
                     GHRLYFAVADVGKTVAVDYSYQDGEDGPVRRITGEMHTIGVTRQTLDSNVGPRDYGFI
                     DLNVPGGGNSIRSIDSVRGQSVEVRVTWPKRGVTNRMHTILQTAWGGGLPEFWDEGQE
                     GRMLVQSVSAVVTKPRDL"
     gene            4314..>4751
                     /locus_tag="PLY56_07650"
     CDS             4314..>4751
                     /locus_tag="PLY56_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ21393.1"
                     /translation="MSAYIADCEWRGAGGRSGDPEAAARPWKSAICSSRGMTLPEILA
                     AIAILSILFGLLFMPLMKAFGYLHTANTAISVQNSARTLFESVTREISDAIYIYDNCD
                     DPTLASLIVVLPQGAGTNVSQVPSTGSSVPILINPGAGLDYGVN"
BASE COUNT          875 a         1468 c         1583 g          825 t
ORIGIN      
        1 cccttggagg atgcagtcgg gttcgtggcc gcggtgggag tgcggtggtg cggccaggcc
       61 cgtatgggtg atgcgcgctc cgggtcggcg cgccatggga gaacgggtga cttatcacta
      121 ccacactgtt ctcacaagaa cggaggaatg gacgaagatg aagacgaaac aacacggctt
      181 cacactcatc gagctcctca ccgtcattgc gatcatcgcg atcctcgcgg cgatcctcct
      241 gccggtgttc ggacgcgcgc gtgagcaggg ccggcgcacc tcgtgcatga gcaatctgca
      301 ccagattgcc ctggccatga agatgtacaa gcaagactgc cgcggctatc cgctcgactg
      361 gccggagacg gttgccggac atcgcccctt ctatacgggc tctcgctggt acggcaacga
      421 cagcaagacg cagccggggt acggcatcgc cacgctctac cccgactacg tgcccaacct
      481 ccgggtcttc aactgcccga acaacgatga gacgaacccg aagtttagca acggcgtcga
      541 tggcaaggat tacgcgcact ggatcggcga cggcgacgag aacaactacc ggcacttcaa
      601 ctcctatgac ggcatggatc cgctcttcgt gatctcggga gatgaagagc tgaccgccca
      661 tggctccgcg tataagtatc gccgccggcc atgggccaca gacgcctaca cgggcggtag
      721 tgccgtcatc tcgcgtgacc aggccaagcg gatgctgatc tggcggaacc catccgagga
      781 caccgtgatc acctggtgct cgcagcaccg cgcggatccg tactcgggca cgccccgcag
      841 tggcgacctg gacctgatcg tctacctcga cggcagcacg aaaaccgtca agtcgaatcc
      901 tggcgccggc gagtccgggc atggctcgaa cgccgctgga gcgcagtagt agccgagagc
      961 gatgtgcctt cgccgtcctc ggacctgtgc tccggggccg gcgaagggac gcgcattccg
     1021 ggagatggag agagcgatgc gcacgtcaag agcaggcttc acgctggtcg aaatcctgac
     1081 ggtcatcgcc atcatcgtca tcctggcggc ggtgctgctg ccggtgtttg ggcgcgcgaa
     1141 ggaacagagt aaccgcaccc actgcctgtc gaacctccac cagattgcta cggccatcaa
     1201 gctgtataag ctggatgagc ggggctatcc ctacgatctt caggagacgg tggcgagccc
     1261 atcacctcgc ccctggatcc cgggcgagcg gtggtttgga agcggcacgg atgccgatgg
     1321 cgacggcttg gtcgatacgc cggataagca acaccccggc tatgggctcg cgacgctata
     1381 tccccactac atcgagaata ttcgggtgtt caactgcccg aacaacgaga cggacgtgct
     1441 gaaagactcg gagatggagg atcctcccga tccgcccatc accgaggcgc aagcggaggc
     1501 tctcacgcgc tggtcgttcc aatcgtacga tgggtgggac acggcactca accagctgaa
     1561 gtacgatcgg tactggcgga atgagcccgg cccgagcgac ccgcccgacc ggcactacat
     1621 ccgccagctg cggtggcgct atcctcccga ggataccgtg ctcacctggt gtactttcca
     1681 ccgtgcggtg atcgatccca cggacccgca gcttgagaac ctgcggaaga ccgaccgcga
     1741 tctcgtggtc tacctggatg gcaccactcg ggtggtgccg tcgtgggcgg aggttgccga
     1801 acagggatcc gggcacctct cgactccggg agagcaggag taggcgcgag aggctatccg
     1861 agaggcattg ccccggcggg agcaacgcgc gcctcgccga aagggcgttc cggggcagct
     1921 cttcgttcag gggcagagtg cgtgatgggg tgggagccaa cccgccgggt tctccttgcc
     1981 ccgatggtaa cggaaccgga tgtggcggcc gcgggattcc cgcggccgcc gcgcacgttt
     2041 gttagcatga cgcggccagc gtcggaggca ggcgccagtt gaacgcaccc ttgaggtgca
     2101 gcgggctcct tgcggaacaa tcatttctgg cgccgcgtct aacgcctaga gaggccgtgc
     2161 agggcagcgg tcgagaacta cgctgaggag tggcctcccg atccgcgccg gcgcagccgg
     2221 acgggatgcg ggagacgaag gcatgaggag cgatcatgcg cgctttcgag agtgacatca
     2281 cgggcaaggt actagccggg attacgcggc cgggcgcgac gcgcgtaggt gagcgcggca
     2341 cgacgctgct tgaggtcctc gtggtgtcac tcgtcttgct ggtgggcatc ctcaccgtca
     2401 tccgcgtttt cccgaccggc ttcgatgtgg tagagcgcgg cgcctacgtc acgatggcga
     2461 atcgctacgc aaaaacgctc ctcgatgatc tctgctcgcg gccggaggcg cttcctgacg
     2521 gcgtgctccc tgcggtctac ctgagcacgg agtatcaggg aacgcggtgg tggatcaacg
     2581 atgaccaggt gcagacgtcc gatctggagc cgcgcccgcc gggcaccggc gatggaggcc
     2641 tgcgcgcctg ggccaacagc ctggtgcagt tccgccgcat ccatcaggag acgactcgcg
     2701 tgcctcgggc ggcctaccgt gatactgacg gcagcctgtt ggcgcgttac gcgctccggt
     2761 tcgcgccgat agagttctac aacagcgaaa cccccggcta ttacgacttg atgaccgttt
     2821 acagcctgcg gccttatgtc aaggtggacc gggatatcct cgaacgctac gccggccggg
     2881 acggatacta cgccatcgat ccggatgagg aggacgagca gcacgcgggc gccccccggc
     2941 agatctattt cagccgggtg agctatcggc gcaccttcaa gatcgatttc gccgtgcgcc
     3001 ccgtcaacgt gaacccgaac gattacaatc ccgacgatgt ggagtatgtg accgaccgga
     3061 tcgtttgggt ggatgccgac tcggacgtgg cggaggacgc cgcaggtgtg gtgaacctgg
     3121 gcgatggggt ctctaccgtc ttggtgcccg aatccgaggt cgtcctgcgc cggcttcgtg
     3181 acgcgcgcgc cgagcccggc agcggcgatc ccctgtggta ccggctgatc aatgcgaccg
     3241 tgggcctcct cgagttcggt ccgggcacgg ccgggttgcc cgtgggcatc aattactcgg
     3301 tgatggactg gcagatcctg catgacgacg tggatgtgcc gatggagccg gacccagggc
     3361 agacggcacc tgcggaggag cagcttgcct gggtgaagct gagtttcccc ttcatcgagc
     3421 gcaacccgat cggcgccccg gaggatgacc ggcccggcat gcaggaggcg atcatcctgc
     3481 agaatatcga cccgaacagc cccaattacg gggaagtgat cgatccgaac aacgataaca
     3541 acggcgctga tgactatccc acctggacgt tcgatgatcc ccagcgggct accgataact
     3601 cactcgccgg ccgcctgggg ttgcgcgtag cgaagttcaa ccaactcggc gtccgccgtg
     3661 tccgcgtctt ctatcgcacg cgtgatggct tcgccgcgat ggtcgtcaag ccatatgccc
     3721 gctatacccc ggtgcctgct gccgcgcgcg tgccgagcta caaccgctat cgttccagcg
     3781 ggcaccggct ctatttcgcg gtggcggatg ttggcaagac cgtggcggtg gactacagct
     3841 accaggatgg cgaggacggg ccagtgcgcc ggatcaccgg cgagatgcac accatcggcg
     3901 tcacccggca gacgctcgac tcgaatgtgg gcccgcgcga ctatggattc atcgacctga
     3961 acgttccggg gggaggcaat tcgattcggt cgattgactc ggtgcgcggg cagtcggtgg
     4021 aggtccgggt gacatggccc aagcgcggcg tgaccaaccg gatgcatacc atcttgcaga
     4081 ccgcctgggg cgggggattg ccggagtttt gggacgaggg gcaagagggc cgcatgttgg
     4141 tgcaaagtgt ctcggcggtg gtgacgaagc cgcgagacct gtagcctccg cccttcgcgc
     4201 ggtggaggcg agagccgcgg gttggcgact gatggcccgc cgtttggtgc ggggaggact
     4261 ggatgttagg acgcggcctg gggagccgtg aagagatggg attggagccg ctgatgagcg
     4321 catatatcgc ggattgcgag tggcggggtg cgggtggccg ttcgggcgat ccggaagccg
     4381 cggcgcgacc ttggaagtcc gcaatctgca gttccagggg aatgacgctc ccggagatcc
     4441 tggcggcgat tgccatcctc agcatccttt tcggcctgct gttcatgccg ttgatgaagg
     4501 cgttcggcta tctgcacacc gccaacaccg ccatcagcgt tcagaatagc gcgcgaaccc
     4561 tcttcgagag cgtgacgcgc gagatctcgg acgccatcta tatctacgat aattgcgacg
     4621 atcccacgct cgcttcgctg atcgtcgtcc taccccaagg cgccgggacg aacgtcagcc
     4681 aggtgccttc cacgggcagc tcggtgccga tcctgatcaa tccgggcgct gggctcgact
     4741 atggcgtgaa c
//
LOCUS       DAOSCV010000258         4721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11428_length_4721_cov_3.055722, whole genome shotgun sequence.
ACCESSION   DAOSCV010000258 DAOSCV010000000
VERSION     DAOSCV010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11428_length_4721_cov_3.055722"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PLY56_07655"
     CDS             complement(<1..713)
                     /locus_tag="PLY56_07655"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ21394.1"
                     /translation="MARIAERPPIVIGVDLGNASCRMAPWRNVNSHSPTPDTMGFRSC
                     PAVVARDHEEGGWLAGASALEIAYRDGVELFDHLKDGILQERGANARKTAVQRLLSKL
                     KFDLQAECAARVIDAVLSVPYSYTLKQRRALRNACSLAGLPVAGLINEPEAAVLGYRI
                     DEQIEEGSERILVFDAGATRCTASVVRLSKQGAYIDIELLSEAHAVTEEGEPVGGDRI
                     DARLLEMFERGRLQIPPLPS"
     gene            complement(718..2835)
                     /locus_tag="PLY56_07660"
     CDS             complement(718..2835)
                     /locus_tag="PLY56_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ21395.1"
                     /translation="MANAISVGIDLGTSTCEVAYWDSVARRIRNVSFGGEGGPIVPSA
                     VWFDRERGRTVVGEQAYVMRVPKHREVVLNIKRQMGTDYRFALGGREYTPAEISGEIL
                     KYLKAEAERFTDGEVKQAVITVPAKFDTLAREHTEEAARIAGLETLMLLPEPVAALIG
                     YESVAQDPEREYVAMVYDFGGGTFDCSVVHARGRQYRVLSKAGVHRLGGAQIDERIAD
                     QLIRPKVLHAGFDLASEEEWALKIRAKILEVAEAAKKSLSDESRRSVVAVLPHLVAPG
                     RPPQTVRVEITQEDLQKTIADLLRVANEQMALALENAGISRAELDMVVLVGGSTRLPA
                     IREAVRQFMGEKAFDGRDGGVRKVLADANPDLVVSRGAARVATSYDLRDERIELVDIL
                     LDTVYLRVPGSLTQFRPLFARGTEIEQAKRTLRLQWPRGDTVAVDLYEGEKDGAPGAK
                     YVGTLKLGPASVPKGTPVELTIQYRTDRALPEVTARVRKTGTPIRATVEYEQHSVQAS
                     GSARLQVTPELDLVFCIDTTGSMVHGDHLEKLRHCCTRFVNALAESDLDLRLAAISFG
                     DRAGGERETVYSFTPDPRLFITRVGSLNENDGGDERESALDALAVALRLPFREGDTRH
                     HIVLVTDAPPHRPEKGSLEQLVSQLAAHNIRVDVVGPFCQEYDTVVAGTGGRLYNIDD
                     ELDQLFADLEESIGHASSGTEGA"
     gene            complement(2835..3728)
                     /locus_tag="PLY56_07665"
     CDS             complement(2835..3728)
                     /locus_tag="PLY56_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21396.1"
                     /translation="MAGFTLALGEPARAAMYQPLLRRAVAAEWEMAFARLAAGGAGAV
                     AGIVSREGEGDLQEGMFALPRLVHRTGIEPYRALFLAALHDRILDRQEHGVCLFIDWD
                     TGGLGVYAVDFADGNARILTSSFQPAICTVALGEAVARHVLLPAAMPGGASLWNDLES
                     EERARLRGAGEGLLRRCWLGSRERFVCAIPPVPRGRALARGAVLSITREALFAQCRLA
                     LATAVDALSGALETVMSRCDTPLRVVVSGEAARWHEFQSAVRRTCGDLPVHLAPRPES
                     FAARGAALAVAEEHLGTKGTV"
     gene            complement(4030..>4721)
                     /locus_tag="PLY56_07670"
     CDS             complement(4030..>4721)
                     /locus_tag="PLY56_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21397.1"
                     /translation="VFHNRRWDFDFATVRQVIAEGWIGEPFLYESSITGYGAPRGWRA
                     DAGAAGTLLADWGAHLIDQALLLVDADVESVWCDVQARKWEATSGSHGLLQMRFATGV
                     LFRVEVSRLAKAPKPRWYILGEEGGLVKTGRDPQEPAMIAGDIDAAREDPADRARVFT
                     QRGGLSAEMVVESLRPSWKSYYQNIADVLLKGTELAVKPEEARRVVAVLDAAAESLRT
                     RASVAVASTWG"
BASE COUNT          778 a         1676 c         1506 g          761 t
ORIGIN      
        1 gaggggagcg gcgggatctg cagccggccc ctctcgaaca tctcgagcag ccgggcgtcg
       61 atcctgtcgc cgccgaccgg ctctccctcc tctgtaactg cgtgcgcctc gctcaggagc
      121 tcgatatcga tgtaggcccc ctgcttgctc agccggacca cgctcgcggt gcaccgcgtg
      181 gccccggcat cgaagacgag aatccgctcg ctgccttcct caatctgctc gtcaatccga
      241 taccccagaa cagccgcctc cggctcgttg atcagcccgg ccacggggag acccgccagc
      301 gagcaggcgt tgcggagcgc ccggcgctgc ttcagcgtgt acgagtaggg aacggaaagc
      361 acggcgtcaa tgacgcgcgc ggcgcactcc gcctggagat caaacttgag cttggagagc
      421 aggcgctgga cagccgtctt ccgcgcgttc gccccgcgct cctggaggat gccgtccttc
      481 aggtggtcaa acagctccac cccatcccga taggcgatct ccagggcgct ggcgccggca
      541 agccacccgc cctcctcatg gtcgcgcgcc accaccgccg ggcaggagcg aaagcccatc
      601 gtatcgggcg ttggcgagtg gctgttcacg ttgcgccagg gcgccatccg gcaggaggca
      661 ttccccaggt ccacgccgat aacgattggc ggcctctcag caatccgtgc catggtgtca
      721 tgccccttcc gtgccgctgc tcgcgtgccc gatactctcc tcgagatcgg caaacagctg
      781 atccagctcg tcgtcgatgt tgtagagccg ccctcccgtg ccagcgacca cggtgtcata
      841 ctcctggcag aacggcccga ccacatcgac tcgaatgttg tgggcagcca gttgcgagac
      901 cagttgctct agcgagccct tctccggccg gtgcgggggc gcgtcggtaa cgagcacgat
      961 gtgatgccgg gtgtctccct cgcggaaggg aagccggagc gccacggcca gtgcatcgag
     1021 cgccgattcc cgctcgtctc ccccgtcgtt ctcgttcagg cttccaacgc gcgtgatgaa
     1081 gaggcgcggg tccggggtga aggagtagac cgtctcgcgc tcgccgccgg cgcggtcgcc
     1141 aaagctgatc gccgccaggc ggagatccag atcactctcg gcaagggcgt tgacgaagcg
     1201 ggtgcagcag tggcgcagct tctcgaggtg gtcgccatgc accatgctcc ccgtggtgtc
     1261 gatgcagaag acgaggtcca gctccggggt cacctgcagc cgtgccgatc ccgatgcctg
     1321 gacgctatgc tgctcgtact ccaccgtggc ccggatgggg gtgcccgtct tcctcacccg
     1381 cgccgtcacc tctgggagcg cgcggtcggt gcggtactgg atggtgagct caacgggagt
     1441 ccctttgggc acagaggccg gacccaactt gagggtgccc acgtacttcg ctcccggggc
     1501 gccgtccttc tcgccctcat agagatcgac ggcgacggtg tcgcctcggg gccactgcag
     1561 ccgaagcgtg cgcttcgcct gctcgatctc ggtgccccga gcgaagagcg gcctgaattg
     1621 agtgagggaa cccggcacgc gcaggtagac agtgtcgagg aggatatcga ccagctcaat
     1681 tcgctcgtcg cgcaggtcat aggaggtggc cacgcgcgcg gcgccgcgcg agacgacgag
     1741 atccggattg gcatcggcga gcaccttgcg aaccccgcca tccctgccgt cgaacgcctt
     1801 ctcgcccatg aactggcgaa cagcctctcg aatcgcgggc aggcgcgtcg agccaccgac
     1861 aaggacgacc atatcgagct cggcgcgaga gattcccgcg ttctccaggg cgagcgccat
     1921 ctgctcgttg gccacacgca acaggtcagc gatggtcttc tgcagatcct cctgggtgat
     1981 ctccacgcgc acggtctgag gaggccgccc cggcgccacg agatggggga gcacggcgac
     2041 cacggagcgc cggctttcat cggagaggct tttcttggcg gcctccgcaa cctcgaggat
     2101 cttcgcgcgg atcttgagcg cccactcctc ctcgctggcc aggtcgaagc ccgcatgcaa
     2161 caccttgggg cggatcaact gatcggcgat ccgctcgtca atctgggcgc cgcccaggcg
     2221 gtgtaccccc gccttagaga gaacgcgata ctgcctgccc ctggcatgga caacactgca
     2281 gtcgaaggtg ccgccgccga agtcgtagac catggcgaca tactcgcgct cgggatcctg
     2341 ggccacgctc tcatagccga taagcgcggc cacgggctcc ggcaggagca tcagcgtctc
     2401 caggccggcg atccgcgccg cttcctcggt gtgctcgcgc gccagcgtgt caaacttggc
     2461 cggcacggtg atcaccgcct gcttcacctc gccatcggtg aagcgttcgg cctccgcctt
     2521 cagatacttg aggatctcgc ccgagatctc ggcaggcgtg tactcccgcc cacccagcgc
     2581 gaaccggtag tcggtgccca tctggcgctt gatgttgagc acgacctccc ggtgtttggg
     2641 cacgcgcatc acgtaggcct gctcgcctac gaccgtgcgt cctcgctcgc gatcaaacca
     2701 caccgcggag ggcacaatgg gcccgccttc accgccgaag gagacgttgc gtatccgccg
     2761 ggctacggag tcccaatagg cgacctcgca cgtgctggta cccagatcga tccccacgga
     2821 aatggcgttt gccatcacac ggttcccttc gttccaaggt gctcctctgc caccgccagg
     2881 gcggcgcctc gcgccgcgaa agactccggc cttggcgcca gatgcaccgg aagatcgccg
     2941 catgtccggc gcacggcgct ctgaaactcg tgccagcggg ccgcctcgcc cgacaccacc
     3001 acccggagcg gcgtgtcgca gcggctcatc accgtctcca gcgcaccact cagcgcgtca
     3061 accgccgtcg caagggccag ccgacactgc gcgaagagcg cctcgcgcgt gatcgagagt
     3121 accgcgccgc gggcgagagc ccggcccctc ggcaccggcg ggatcgcgca cacgaaccgc
     3181 tcccgacttc caagccagca ccgccgcagc aggccctccc ctgccccgcg gagacgcgct
     3241 ctctcctcgc tctccagatc gttccagagg ctggccccgc ctggcatggc tgccggcagg
     3301 agcacgtgcc tggcgaccgc ctctcccagc gccaccgtgc agatcgccgg ttgaaatgag
     3361 gaggtgagga tgcgcgcgtt cccatcggcg aagtcaacag cgtagacgcc aagcccaccg
     3421 gtatcccagt cgatgaagag gcacacgccg tgctcctggc gatcgaggat ccggtcgtgg
     3481 agcgccgcga ggaagagggc acgatagggc tcgatgccgg tccggtgtac gaggcgcggg
     3541 agagcgaaca tcccctcctg gagatcgccc tcgccttcgc gagacacgat gccggcgacc
     3601 gcccccgcgc cgcccgcggc cagccgggca aaggccatct cccactccgc cgcgaccgcg
     3661 cggcgtagca ggggctgata catcgctgcc cgtgccggct ctcccaacgc cagggtaaac
     3721 cccgccacga tgggttgctt cctggcagga agaggtcgtg tcactcccgc gttcccccaa
     3781 ctcgatcgcg ctgagtgatt ggcgcggccc aaggggtctt cacgtgcggt agctccatga
     3841 aggcatgaga acacctcttc gtgatttcaa cttctatgac gagtggcgct actccttccg
     3901 caaccagtga tacgcgcggg cgtcacggtc agagaagctt tgacagcgcg gggagggacg
     3961 ggttgggggc ttccatccat cctccgggca accgcggggc ggctggcggg cgatgcgcga
     4021 gaagaacgcc tatccccagg tgctggcgac cgccacgctg gcgcgggtac gcagcgactc
     4081 cgcggccgcg tccagaacgg cgacgactcg gcgcgcctct tccggcttca cggcgagctc
     4141 cgtgcctttc agaagcacat cggcgatgtt ctggtagtac gatttccagc ttgggcgcag
     4201 gctttcgacc accatctcgg cggagagccc gccgcgctgg gtgaagacgc gagcccggtc
     4261 ggcggggtct tccctcgccg cgtcgatatc gccggcgatc atggcgggct cctgtgggtc
     4321 acgccccgtc tttaccaggc ccccctcttc cccgaggatg taccaccgcg gcttgggcgc
     4381 cttcgccagc cggctcacct cgacgcggaa gagcaccccg gtggcaaacc gcatctgcag
     4441 gaggccatgg ctgccgctgg tcgcttccca tttgcgcgcc tgcacgtcgc accacacgct
     4501 ctcgacgtcg gcatcgacta ggagaagcgc ttggtcaatc aggtgggcgc cccaatcggc
     4561 caggagcgtg ccggccgccc cggcatcggc gcgccagccg cgcggcgccc cgtagccggt
     4621 gatggaagac tcgtagagga aaggctcgcc gatccatccc tccgcgatca cttggcgcac
     4681 ggtagcaaaa tcaaagtccc agcggcgatt gtggaagacg c
//
LOCUS       DAOSCV010000259         4669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11591_length_4669_cov_3.382315, whole genome shotgun sequence.
ACCESSION   DAOSCV010000259 DAOSCV010000000
VERSION     DAOSCV010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11591_length_4669_cov_3.382315"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..483
                     /locus_tag="PLY56_07675"
     CDS             94..483
                     /locus_tag="PLY56_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HOJ21398.1"
                     /translation="MAQGHGHKLWIIPDGYIATTPPDAVGPSGYVTHESVCILNDGDE
                     PAQCTLDVYFEDRDPMLGIPFTVGAQRSLHLRLDRLKTPSGEEIPRDTCYSLRVTSDR
                     NIVVQHSRLDVTQSNATLFTTMAFPVE"
     gene            complement(504..1070)
                     /locus_tag="PLY56_07680"
     CDS             complement(504..1070)
                     /locus_tag="PLY56_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004968729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOJ21399.1"
                     /translation="MVAEPHNNPGGWANHLRRLGAHYMRDLVFGANDGIITTFAVAAG
                     VAGAQMPNRVVLILGFANLLADGVSMGASNFLSIRSEHAANPNEENADGALDPVKHGF
                     ATFLAFVVAGTIPLLAYLVPHLFRTPPFIDAVLLTIVALFGVGAARTFVYPRCWWLSG
                     LEMLVVGAIAAGAAYGVGHFLAGLVNSV"
     gene            complement(1134..1571)
                     /locus_tag="PLY56_07685"
     CDS             complement(1134..1571)
                     /locus_tag="PLY56_07685"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOJ21400.1"
                     /translation="MTDLELARAERERLGLALAIARDGEVIATGNRGGVADLLEMLRR
                     TDLELAGASLSDRVVGRAAAMLARCAGIVAVQGGIMSEPAIQALRDAGIPYSAERQVP
                     HILNRAGDGMCPMERLCLEQTDPHACREAIEAFVACQASRARP"
     gene            complement(1656..>2402)
                     /locus_tag="PLY56_07690"
     CDS             complement(1656..>2402)
                     /locus_tag="PLY56_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HOJ21401.1"
                     /translation="IPERQAALDRADEELRALGFDPAVYAQHRQLCRALEMRRETRLQ
                     EFHRADRERERALAEQQQADARYDDYQCQVEIIHERRLEKENRDELLALISAFRVDLI
                     GRIRPTLATKASRLVRDLTDGKYDQIELDEDYNLWMHDDGERRELRSFSGGENDLVHL
                     CVRLAISQMIAESAGGAEHGFLVLDEVLGSQDGERRGLILQALGALSRQFRQILMITH
                     LDDVKEMVEHAIELRELPNGSSEVVASNGM"
     assembly_gap    2403..2412
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2413..4054)
                     /locus_tag="PLY56_07695"
     CDS             complement(<2413..4054)
                     /locus_tag="PLY56_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOJ21402.1"
                     /translation="MRLISLDLENFLKFERLALEFPDGLTGILGPNGVGKSSLVEAIA
                     WALYGSPVSRTRNEGLPFRRNVPCRVALTLEIEGDEYRVVRELRGASLTAHAEVSING
                     VPAEVGTERTNRLIASRLRMDHRAFMVSFFARQKELDALIAWEPRDRKRYILRMLGLE
                     RVDRAIEALRRDITDAERDLRAREAALPSLDAIQAARKAARERVAALTRQREEAQAAL
                     EATDRELSEARQRAEEMEAQQRRYTAAERARDLARQALEAARREEAIYAGRVRDLHAK
                     QEELRRLEPSLQPLPELRARRQALELAAGLQRRRNALVRTIAHHEKTGQDLLKQAASL
                     SKELSTRDGLQLAHNQAEALLAQTRAELTDIQGQIRSNEARLMQIKRERQQLDHDLKS
                     VKELGAAGRCPTCQRVLDASYTEVVSHLEGELKALDGERDDYTRFLAEDSARATQLQR
                     QVADTEAERQRLNLALRRVAQAEARLSEVQSAIARNTAELARAQAELEEMGEQEYDPA
                     AHQRVVDQLATLEKVEHRVIGLRQEVERLPAEEQALARA"
     gene            complement(4029..>4669)
                     /locus_tag="PLY56_07700"
     CDS             complement(4029..>4669)
                     /locus_tag="PLY56_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21403.1"
                     /translation="FRMAEVNEQILGEDQLPKAMDCVLLGHYHKHVSLQPGVWYAGSI
                     ERFSFGEVGQTKGYCLFDPEDPQPQFREIPVRPMLDLDPIDATEMDADDLLEAMAGRL
                     QAQPLSGAIVRLGLRHVPPATRANLDLRQLRDLVADALHFAIRWEREGDGPEEKEVSS
                     GTFRPLEDEWRSFMETADVHGRDRSALTRAGLEYLTSVVGEDEPDAVDLFGS"
BASE COUNT          738 a         1583 c         1519 g          819 t
ORIGIN      
        1 gcggagcttc gagctccgcg cgtgcgggcg gccttctcct tatctcggaa ttccagggca
       61 gtctcagcga ttctggacaa ggaaggtact cctatggccc aaggacatgg ccacaagctt
      121 tggatcatcc ctgatggcta catcgccacg accccgccgg acgcggtggg cccctcaggg
      181 tacgtcacgc acgagtcggt ttgcatcttg aatgacggcg acgagcctgc gcaatgcacg
      241 ctggatgtct acttcgagga tcgcgacccg atgctcggca tcccgttcac ggtcggcgcg
      301 cagcgctcgc tgcacttacg cctggacagg ctgaagacgc cttcaggcga ggagattccc
      361 cgagacacct gctactcgtt gcgcgtgacg agcgaccgca acatcgtggt ccagcactct
      421 cggctcgatg tgactcagtc taacgccacc ctcttcacga cgatggcttt tccggtggag
      481 tagccggcgc cgcgccgtcg cggctacacg ctgttgacca gcccggcgag gaagtggcct
      541 actccgtagg cggcccccgc cgcgatggcc ccgacgacga gcatctccag gccgctcagc
      601 caccagcagc gcgggtagac aaacgtgcgc gccgctccga cgccgaagag ggcgacgatc
      661 gtgaggagaa cggcatcgat gaagggtggc gtgcggaaga ggtggggcac cagataggcg
      721 aggaggggga tggtgcctgc gaccacaaag gccaggaagg tagcaaagcc gtgcttcacg
      781 gggtcgaggg cgccatcggc gttttcctcg ttcggattgg ccgcgtgctc cgagcggatg
      841 ctgaggaagt tactggcacc catcgagacg ccatccgcca ggaggttggc gaagccgagg
      901 atcagcacca cgcggttcgg catctgagca ccggcaacgc cggcggccac ggcgaaagtg
      961 gtgatgatcc cgtcgttggc gccaaagacg aggtcgcgca tgtaatgcgc gcccagcctg
     1021 cgcaggtggt tggcccaccc cccggggttg ttgtgcggtt cggccaccat tgccgggctt
     1081 tttcccgcga gcggtgttta ccaaaccacg ggccaggcag agctcagcgc cgcttagggc
     1141 cgggcgcgcg aggcttggca tgcgacaaac gcctcgatcg cctcgcgaca ggcgtgggga
     1201 tcggtttgct ccaggcagag gcgctccatc gggcacatgc cgtcgccggc acggttcagg
     1261 atgtgcggca cctggcgctc cgcggagtag gggatgcccg catcgcggag cgcctggatg
     1321 gccggctcgc tcatgatccc gccctggacc gcgacgatcc cggcgcagcg cgcgagcatc
     1381 gccgccgccc ggccaaccac gcggtcggag agcgacgcgc ctgccagctc cagatcggtg
     1441 cgccggagca tctccagcag atccgcaacg ccgccgcggt tccccgtcgc gatcacctcg
     1501 ccgtcacggg cgatggcgag tgccaatccc agccgctctc gttctgcgcg ggccaactcc
     1561 aaatctgtca tgcttcctcc tgttaccaat cccaacacca cccgccggat gtcatgggct
     1621 gcctgctagc gggataacgg cgccccctcg ccggctcaca tcccatttga tgccactact
     1681 tcgctggatc cattgggcag ctcgcgcagc tcgatggcgt gttcgaccat ctccttcaca
     1741 tcatcaaggt gggtgatcat taagatctgg cggaactgcc tgctcagcgc gccgagtgcc
     1801 tggagaatga ggccacggcg ctcgccatcc tgacttccga gaacttcgtc caggacgagg
     1861 aagccatgct cggcgccgcc ggcgctctcg gcgatcatct gcgaaatggc caggcgcacg
     1921 cacaggtgga cgaggtcgtt ctcgccaccg ctgaaggagc gtagctctcg gcgctcgccg
     1981 tcatcgtgca tccagaggtt atagtcctca tctagctcaa tctgatcata cttgccgtca
     2041 gtaaggtctc gcacgaggcg cgacgctttg gtggcaagcg tcgggcggat ccggccgatg
     2101 agatccaccc ggaacgcgga gatcagcgcc agaagttcgt cacggttctc cttctccagg
     2161 cgcctctcgt ggatgatctc gacctggcac tgatagtcat cgtatcgtgc atccgcctgc
     2221 tgttgctcgg ccagggcgcg ctcgcgctcg cggtcagcgc ggtggaactc ctgcaggcgt
     2281 gtttcgcgcc gcatttccag cgcgcggcag agctggcggt gttgggcata caccgcgggg
     2341 tcgaatccaa gggcgcgcag ctcctcatcg gcacgatcca gcgcggcctg gcgctccggg
     2401 atnnnnnnnn nngggcacgg gcaagcgcct gctcctcagc gggcagccgc tccacctcct
     2461 ggcgtagtcc aatcacccgg tgctccacct tctccagcgt cgcgagctgg tccacgacgc
     2521 gctgatgcgc cgccggatcg tactcctgct cgcccatctc ctcgagctcc gcctgcgctc
     2581 gggccagttc cgccgtgttg cgcgcgatgg ccgattgcac ctcggataga cgtgcctctg
     2641 cctgggccac ccggcgcagg gcgaggttga gccgctgtcg ctccgcctcg gtatccgcca
     2701 cctggcgctg cagctgcgtg gcgcgcgcgc tatcctccgc gaggaagcgc gtgtagtcat
     2761 cgcgctcgcc atccaacgcc ttcagctcgc cctctagatg gctgaccacc tcggtgtagg
     2821 atgcatcgag cacacgctgg caggtggggc atcttcccgc ggcgcccagc tccttcaccg
     2881 atttcaggtc gtggtcgagc tgctgccgct ccctcttgat ctgcatcaag cgcgcttcgt
     2941 tgctgcggat ctgcccctgg atatcggtga gctccgcgcg cgtctgtgcc aggagcgcct
     3001 cggcttggtt gtgggccaac tgcaagccgt cgcgcgtcga gagttccttg ctgagcgagg
     3061 cggcttgttt caggaggtcc tgtccggttt tctcgtggtg tgcgatggta cgcaccaggg
     3121 cattccgtcg gcgctgcagg cccgctgcga gctccagcgc ctggcggcgc gcgcggagct
     3181 ccgggagcgg ttgcagcgag ggctcgaggc ggcgcagctc ttcctgcttg gcgtgcaggt
     3241 cgcgtacgcg tccggcgtag atcgcctcct cccgacgggc ggcctccagc gcctggcggg
     3301 ccaggtcgcg cgcgcgttcg gccgcggtgt atcgcctttg ctgcgcctcc atctcctcgg
     3361 cgcgctggcg cgcctccgat agctcgcggt ctgtcgcttc gagggcggcc tgggcctcct
     3421 cgcgctgacg ggtgagcgcc gccacgcgct cccgagccgc cttacgcgcc gcctggatcg
     3481 catccagaga gggaagcgcc gcttcgcgcg cccgcagatc gcgctccgcg tcggtgatgt
     3541 cgcgacgcag cgcctcgatg gcgcgatcca cgcgttccag gcccagcatg cgcaggatgt
     3601 agcgcttccg atcccgcggc tcccacgcga tgagggcgtc tagctccttc tgacgggcaa
     3661 agaaggagac catgaaggcg cggtgatcca tgcggaggcg ggatgcaatg agccggttgg
     3721 ttcgctcggt gcctacctcg gcgggcaccc cgttgatcga aacctcggcg tgagcggtga
     3781 ggctggcgcc gcgcagctct cgcaccacgc gatactcatc cccctcgatc tccagggtga
     3841 gggcgacccg gcagggtacg ttgcgccgga aagggagccc ctcgttccgg gtgcggctga
     3901 ccggcgagcc ataaagcgcc cacgcgattg cctcgacgag ggacgacttc cccacgccgt
     3961 tgggcccgag gatgccggtg aggccatcgg ggaactccag agccaggcgc tcgaacttca
     4021 ggaagttctc aagatccaaa gagatcaacc gcatccggct catcctcccc gaccacgctc
     4081 gtcagatact ccagaccggc gcgcgtcagc gcgctccgat cgcggccatg cacatcggcc
     4141 gtctccatga aagagcgcca ctcgtcttcc agaggacgga acgtgccgct cgagacctcc
     4201 ttctcctccg gtccatcgcc ttcacgctcc catcggatcg cgaaatggag cgcgtcggca
     4261 accaggtcgc gcagctgccg caggtctagg ttggcgcgcg tggcaggtgg aacatggcgc
     4321 agccccagcc ggacgatggc gcctgaaagc ggttgtgcct gcaggcgccc ggccattgcc
     4381 tcgaggaggt catcggcatc catttccgtg gcatcgattg gatccagatc cagcatgggg
     4441 cgcaccggga tctcgcggaa ttggggctgg ggatcctccg ggtcgaagag gcaatagccc
     4501 ttggtctggc caacctcgcc aaagctgaaa cgctcgatgg agccggcata ccacacgccg
     4561 ggctggagcg atacgtgctt gtgatagtgg ccgaggagca cgcaatccat cgccttcgga
     4621 agctggtcct cgcctaggat ctgctcgttg acctccgcca tgcgaaact
//
LOCUS       DAOSCV010000260         4658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11632_length_4658_cov_2.870302, whole genome shotgun sequence.
ACCESSION   DAOSCV010000260 DAOSCV010000000
VERSION     DAOSCV010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11632_length_4658_cov_2.870302"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /locus_tag="PLY56_07705"
     CDS             <1..1388
                     /locus_tag="PLY56_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ21404.1"
                     /translation="EQHIRRVLEALFRRDGYEVYTAETPQRALALAAETDIDVLVADL
                     IMPDLTGVQLMQEIRQLHPGVATVMITAHGSVRSAVDAMKLGAFDYIAKPFDMDELLR
                     VVRKAIDWKRLNGEAKCARELPDAEWTLDNIVGSSPQMQQVCRIVRRAANSRATVLLR
                     GESGTGKELVALALHKNSPRARHPFVPVAAAALSEELLESELFGHEKGSFTGATNQRI
                     GRFELADGGTLFLDEIGDISPALQVKLLRVLQEREFERVGGTKTIKVDVRLIAATNRD
                     LEAAVAAGRFRHDLYYRLQVIQITMPPLRQRKEDLPALVDHFIRKYAGEDGRQIQGVS
                     DEAMEILTRHSWPGNVRELENCIEYAVVMSEPDATLITPDVLPLSVRGLVASGDTIQV
                     PAGTTFDEAVAVVERELLLDALKKTGWNLRAASAHLGMSDRSLEHYIRKHGLESQEDW
                     MPKVPPAIRRG"
     gene            1960..2763
                     /locus_tag="PLY56_07710"
     CDS             1960..2763
                     /locus_tag="PLY56_07710"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOJ21405.1"
                     /translation="MSERAEIGVFGGSGFYAFLDKVEEVKVETPYGAPSDRIALAEVH
                     GRRVAFLPRHGREHSLPPHKIPYRANLWAMKSLGVKRIIAPTAAGSLQPDVHPGDFVI
                     CDQFVDRTYGRADTFYDGPVVTHISTAAPYCPQLRALAAQVGEAQGVRVHRTGTVVVI
                     QGPRFSTAAESRWFSQMGWHVVNMTQYPECVLARELEICYANISLITDYDAGLVGLPG
                     IEPVSVEKMLEVFHRNNARVKELILGMIEAMPEARDCDCGCALRTARIE"
     gene            2763..3839
                     /gene="mtnA"
                     /locus_tag="PLY56_07715"
     CDS             2763..3839
                     /gene="mtnA"
                     /locus_tag="PLY56_07715"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOJ21406.1"
                     /translation="MATPDELRPVEWADGAVRLIDQTRLPHAFVVKEYRDYREVAEAI
                     RTMEVRGAPAIGAAAALGIALAALEGADLAPEAFRAHMDEAARVLASTRPTAVNLFWG
                     INRTMAAVAAAGAGSPAEAAKRAVEEACAILEEDITVNRAMGRHGAALIRDGDTVLTH
                     CNAGALATVGYGTALGVIRAAVEQGKRIRVFADETRPRLQGMKLSAWELAREGIDVTI
                     ISDNMAPALMRRGLIQCAVVGADRVAANGDVVNKIGTYGVALACRAHGIPFYVAAPLS
                     TVDLQMPSGDEVPIEERASEEVTHVDGVRIAPEGVPVLNPAFDVTPAELVSALITEVG
                     VLRAPYEAALREAVAGAARPGEER"
     gene            3842..4363
                     /locus_tag="PLY56_07720"
     CDS             3842..4363
                     /locus_tag="PLY56_07720"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOJ21407.1"
                     /translation="MTLDEIRALIRTVPDFPKENIMFRDITPVLRNPAALRAVIEHLA
                     DCCRDDKVDVVVGIESRGFMFAVPLALALGAGFVPVRKLGKLPAEVVQAEYALEYGTN
                     TVEMHRDAIQPGDRVIIVDDLIATGGTAGAAIDLVEQLGGEICRLLFLVELAGLKGRE
                     GLQGYEVHSLITF"
     gene            4335..>4658
                     /locus_tag="PLY56_07725"
     CDS             4335..>4658
                     /locus_tag="PLY56_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21408.1"
                     /translation="MRYTVSSRSNRKIDAPLEERKQRILRAVVRDYVETAEPVGSEAL
                     AERYDFGVKSATLRNEMAEMAEQGYLQQPHTSAGRIPSDKGYRYYVDWLITPRTPSAS
                     RRQQMR"
BASE COUNT          787 a         1425 c         1631 g          815 t
ORIGIN      
        1 acgagcagca tattcgtcgg gtgttggagg cgctcttccg gcgggatggc tatgaggtgt
       61 acacagccga gacgccgcaa cgcgccctcg cgctcgccgc tgagacggat atcgatgtgc
      121 tggtcgccga tctgatcatg ccggatctga cgggcgtgca gttgatgcag gagatccgcc
      181 agcttcaccc gggcgtggcc accgtgatga tcacggcgca cggctccgtg cgctcggcgg
      241 tggatgcgat gaagctgggc gcctttgatt atatcgccaa gccgttcgat atggacgaat
      301 tgctgcgcgt ggtgcgcaag gccattgatt ggaagcgcct gaacggagag gccaagtgcg
      361 cgcgcgagct ccccgacgcg gagtggacgc ttgacaacat cgtcggctcc agcccgcaga
      421 tgcagcaggt ttgccggatc gttcgccggg cggccaacag ccgggctacg gttttgctcc
      481 gtggcgagag cggtaccggc aaagagctgg ttgcgctggc gttgcacaag aactctcccc
      541 gggccaggca ccccttcgtg ccggttgccg ccgcggctct ctccgaggag cttctcgaga
      601 gcgagctgtt tggccatgag aagggctcct tcaccggggc caccaaccag cgcatcggcc
      661 gattcgagct ggccgatggg ggcacgctct tcctggatga gatcggcgat atctcgccgg
      721 cgcttcaggt gaagctcctc cgcgtgttgc aggaacgcga gttcgagcgt gtcggcggca
      781 caaagacgat caaggtggat gtccggctca ttgccgcgac caatcgcgac ctggaggccg
      841 ccgttgcagc cggccggttt cggcatgacc tctactaccg cctccaggtg atccagatca
      901 cgatgccgcc gctgcgccag cgcaaggagg acctcccggc gctggtggac cacttcattc
      961 gcaaatacgc gggtgaggat ggccggcaga ttcaaggggt ttccgacgag gcgatggaga
     1021 tcctgacgcg gcactcgtgg cccgggaacg tccgcgagct ggaaaactgc atcgaatacg
     1081 cggtggtcat gtccgagccg gacgcgactc tgatcacccc ggatgtgctg ccgctcagcg
     1141 tgcgcggcct cgtcgcctcc ggcgatacca ttcaggtgcc ggccggaacc actttcgacg
     1201 aggcggtcgc cgtcgtcgaa cgcgagttgc tcctcgatgc actcaagaag accggctgga
     1261 atctgcgcgc cgcctcggct cacctcggca tgagcgaccg ctcgttggag cactacatcc
     1321 gtaagcacgg cctggaatcg caagaggact ggatgccgaa ggtgccgccg gccatccgga
     1381 ggggatgagt ccggatcggg ggagagcggg ccccagcggt ccgacatggg ggcctgggag
     1441 ggggagctgc cgcgcgcgga tctagagccc tgcgttgctt ccaggcaaag cccctttcga
     1501 ggctccccct cccagcccac gcctgagccg ctccccaagg gtgtggccag cccacgcacg
     1561 cggagctaga agcatcgtgc tgcttcccgg cgaggcccct ttcggggctc cctcccaact
     1621 tgcgcctgcg ctgcctgccc gcgcatgcgg cctaaccggg cctggcaggg cttcgttcgc
     1681 aagcagcgcg gtgatttacc gccgcgtatg ggatgcggct cgcacctcca tgcgcgtccc
     1741 cctcccatcc tgcccccttc acccgtggca tcgccgccgg cgcggggtcg aagggcaggg
     1801 agggcgccgg gagccgcgcg gatcagccgt gtctcggaat cccggagcgc cttgcatctt
     1861 gtttgacaaa gctcgcgagc gtctgctatc attgagcaag gtggcaccag gcagagcggc
     1921 atccgccaat ccgccatttg agatccagcg ggggtgacga tgtctgagcg agcagagatc
     1981 ggggtcttcg ggggttctgg gttctatgcc ttcctcgaca aagtggagga agtgaaggta
     2041 gagacgccgt atggcgcgcc gagcgaccgg attgcgctgg cagaggtcca tgggaggcgg
     2101 gtggcattcc tgccccggca tgggcgagag cactcgttgc cgccacacaa aatcccctac
     2161 cgggcgaacc tctgggcgat gaaatcgctg ggtgtgaagc ggatcatcgc gccaaccgcg
     2221 gcgggaagcc tgcagccgga tgtgcatcca ggcgattttg ttatttgcga ccaatttgtc
     2281 gaccgcacct acgggcgtgc cgataccttc tatgatgggc cggtggtcac gcacatcagc
     2341 acggctgcgc cctactgccc ccagctacgg gctctggctg cccaggtagg cgaggcgcag
     2401 ggcgtgcgcg ttcatcgcac cggcaccgta gtcgtcatcc agggaccgcg cttttccacg
     2461 gcagccgaaa gccgctggtt ctcgcagatg ggctggcacg tcgtcaatat gacccagtac
     2521 ccggagtgcg ttctcgcgcg cgagctggag atctgctacg ccaacatttc actcatcacc
     2581 gactatgacg ccggccttgt gggtctgccc ggcatcgagc cggtctccgt ggagaagatg
     2641 ctggaggtgt tccatcggaa caacgcgcgc gtcaaggagc tgatcctcgg gatgatcgag
     2701 gcgatgcccg aggcgcgcga ctgcgactgc ggatgtgccc tgagaacggc gaggattgaa
     2761 tgatggcaac gcctgacgag cttcgccccg tggaatgggc cgatggcgcg gtgcgcctga
     2821 ttgaccagac gcgcctgccg cacgccttcg tcgtcaagga gtatcgcgac taccgggaag
     2881 tggccgaggc gatccggacg atggaggtgc gcggcgcgcc cgcgattggc gccgcggccg
     2941 ccctgggtat cgccctggcg gcgctggagg gcgccgacct ggcccccgag gcgttccggg
     3001 cgcacatgga tgaagccgcg cgcgtgttgg catccacccg gccgacggcg gtgaatctct
     3061 tttgggggat caaccggacg atggcggcgg tcgccgcggc aggggcagga tccccggccg
     3121 aagcagcgaa acgagctgtc gaggaggcat gcgctatcct ggaagaggat atcacggtga
     3181 accgggcgat ggggcggcat ggcgccgcgc tgatacgcga tggcgataca gtgctcaccc
     3241 actgcaatgc gggcgcgctg gcaaccgtgg gctatggcac ggcgctgggc gtgatccggg
     3301 ccgccgtgga gcagggcaag cgcatccgag tttttgcgga cgagaccagg cctcgcctgc
     3361 agggcatgaa gctgagcgcc tgggagctgg cgcgtgaggg catcgatgtc accatcatct
     3421 ccgacaatat ggctccggcg ctcatgcgcc gcggcctgat ccagtgcgcg gtggtcggtg
     3481 ccgaccgcgt ggctgccaat ggagatgtgg tgaacaagat cggcacctat ggtgtggcac
     3541 tggcgtgccg ggcgcatggc attcctttct acgtggccgc tcctctctcc acggtggatc
     3601 tgcagatgcc atcgggagat gaggtgccga ttgaggagcg agcgagcgag gaagtgacgc
     3661 acgtggatgg agtgcggatc gcgcccgagg gcgtgccggt gctgaacccg gcgtttgatg
     3721 tcacgcctgc cgagctcgtc tcggcgctga tcacggaggt gggggtgcta cgggcccctt
     3781 atgaagcggc gttgcgggaa gcggtcgctg gcgcggcgcg ccccggagag gagcggtgat
     3841 gatgactcta gacgagatta gagcgctcat tcgcacggtt cccgatttcc ccaaagagaa
     3901 catcatgttc cgggacatca ctcccgtgct gcgtaatccg gcggcgctgc gcgcggtgat
     3961 cgagcacctg gcggattgct gtcgcgacga caaggtggat gtggtggtgg ggatcgagtc
     4021 gcgcgggttc atgttcgcgg tacccctggc tttggctctg ggcgcgggct tcgtcccggt
     4081 gcgcaagctg gggaagctgc ccgccgaagt cgtccaggcc gagtatgcgc tggagtatgg
     4141 caccaacacc gtggagatgc atcgggatgc gatccagccc ggcgaccggg tgattatcgt
     4201 cgatgatctg atcgcgaccg gcggcacggc gggtgctgcg atcgatctcg ttgaacagct
     4261 cgggggcgag atctgccgcc tgctgttcct cgtcgagctt gcggggttga agggccgcga
     4321 gggtctgcag ggctatgagg tacacagtct catcacgttc taaccggaaa atcgatgcac
     4381 ctttggagga gcgcaagcag cgaatcctcc gcgctgtggt acgggactac gtggagacag
     4441 cagagccggt cggctctgag gcgcttgcgg agcgctatga cttcggggtc aaatccgcca
     4501 cgctgcgcaa tgagatggcg gagatggccg aacagggcta tctgcagcag ccgcatacgt
     4561 cggcggggcg cattccgtcg gataagggat accgttacta cgtggactgg ctgatcacgc
     4621 ctcgcacccc cagtgcctcc cggcgccagc agatgcgc
//
LOCUS       DAOSCV010000261         4655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11644_length_4655_cov_3.592174, whole genome shotgun sequence.
ACCESSION   DAOSCV010000261 DAOSCV010000000
VERSION     DAOSCV010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11644_length_4655_cov_3.592174"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLY56_07730"
     CDS             <1..644
                     /locus_tag="PLY56_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21409.1"
                     /translation="IWKVQIHPQRTPLAEDVDFRALAEAYALSGGDIKNAVLKAAQMA
                     AGEEGPDHRKRIHQRHFEAAVQEVLEAKRVMDQSLFGEVDLNGPASAVVTAAFQEVES
                     RITELEKEAEAIPPQIATIRQEVSIVLEGFSARVEGIQEEHAARIAEVKRQLEETRQS
                     MEAQLQALSAQWSERLKRVTLVPLPRWQAYLVGGLGMVAAAAAGAAAKAFGLF"
     gene            complement(752..913)
                     /locus_tag="PLY56_07735"
     CDS             complement(752..913)
                     /locus_tag="PLY56_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ21410.1"
                     /translation="MGHLLCALWAEEDGQTLVEYSLIVALISVTIITALGLVSGKIQA
                     KYSDIRNAM"
     gene            complement(1102..1263)
                     /locus_tag="PLY56_07740"
     CDS             complement(1102..1263)
                     /locus_tag="PLY56_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ21411.1"
                     /translation="MKSLLCKLWVEEEGQTLVEYALIIALIAVAAIAAMSYLSGKVQT
                     NYSSVGNHL"
     gene            complement(1318..1479)
                     /locus_tag="PLY56_07745"
     CDS             complement(1318..1479)
                     /locus_tag="PLY56_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ21412.1"
                     /translation="MKSLLCKLWVEEEGQTLVEYALIIALIAVAAIAAMSYLSGKVQT
                     NYSSVGNEL"
     gene            1759..3276
                     /locus_tag="PLY56_07750"
     CDS             1759..3276
                     /locus_tag="PLY56_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21413.1"
                     /translation="MKPRREALSLETRRFLSLDNVYLAAAVAVIMIVPLLTRVAPHDF
                     WWHVAMGRIIVREGWIPTVDSFSFTRAGEPYFNQGWLAQCVMYALHQAGGLPLLVVVQ
                     AVLMAVAYLLLLWMVVRKTGRLRLAAAVLLLAIEPNAYTNWSIRPQSYAIPLFASFLV
                     ILTGYRQRWWRTLWPLVPMMAVWVNLHGSFPLGLGLMGLVLLGEGIRRKAGDSGALPA
                     REWWRLAGYTVAVALATLANPTGPRVLGYVSHLMTAKSVVNLVTEWAPPTPRDAAGAI
                     FFALVIALFVALIYARRRPALTDLFLMLAFLWLGLSAGRNIVWFAMVAYLPLAEALAS
                     HWSDAPPASKRDGAGQGIPAANAVLVSALATAVVLSLPWIRPLLGRPVSLLTEDTPVK
                     AVEKLRALPPEERPKRLFHTTGFGSYLIWAAPEQPVFIDPRFEFYPVDQINDCRFASA
                     GYNVEETLARYRIDGLLLDRKSQEKLEERARASGRWCEVYRDATAVLMVPARPAP"
     gene            complement(3464..3625)
                     /locus_tag="PLY56_07755"
     CDS             complement(3464..3625)
                     /locus_tag="PLY56_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOJ21414.1"
                     /translation="MKSMLRKLWAEEEGQTLVEYALIIALIAVAAVSAMTYLSGKVQS
                     NYSSVGDKL"
     gene            4104..>4655
                     /locus_tag="PLY56_07760"
     CDS             4104..>4655
                     /locus_tag="PLY56_07760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOJ21415.1"
                     /translation="MRQQCLARVGKITALALVLAGLVWQGTRTPALVDPSAGDYEAYW
                     MAARLLLTGGNPYDLDAVAALQGVPPLQNGSVKVAWNPPWALAVMLPFGLVEFPLSRM
                     LWFLLMVAVLFASTSVFWLRDGGPLSRRWVAALLCILFPPALLALWNGQMTPLLLAGI
                     AGFLHSGAQGRWGRAGAFLALGAL"
BASE COUNT          747 a         1430 c         1587 g          891 t
ORIGIN      
        1 tgatctggaa ggtgcagatc cacccgcagc ggacccctct cgcggaggat gtcgattttc
       61 gggccctggc cgaggcctat gcgctctcgg ggggcgatat caagaacgcg gtgctgaagg
      121 cggcccagat ggcggcgggc gaggaagggc cggatcaccg caagcggatt caccagcggc
      181 acttcgaggc ggcggtgcaa gaggtgctcg aggcgaagcg cgtgatggac cagagcctct
      241 ttggcgaggt ggatctcaac gggcccgcga gcgccgtggt caccgcggcg ttccaggaag
      301 tagagtcacg catcacagag cttgagaagg aggccgaagc cattcccccc caaatcgcga
      361 cgatccgcca ggaagtcagc atcgtgctcg agggcttctc ggcgcgcgtg gaggggatcc
      421 aggaggagca cgcggcgcgc atcgcggagg tcaagcgaca gctagaggag acccgtcaga
      481 gcatggaggc acagcttcag gcgctctcgg cgcagtggag cgaacgcctg aagagggtga
      541 cgctggttcc gttgccccgc tggcaggcct acctggtggg gggcctgggg atggttgccg
      601 ccgctgccgc aggtgccgcc gcgaaggcgt ttggcctgtt ctaggctgtt tgggaggaga
      661 ggttccaggg gccgggccgg cgccgtccgg cccggcttac tgtgggggcg aagccggcgc
      721 cggtgcccga ttcacgcagt gcgcgaggac gctacatcgc gttgcgaatg tcgctgtact
      781 tggcctgtat cttcccgctc acgagcccga gggcggtgat gatcgtgacg gagatgaggg
      841 cgacgatcag gctgtattcg accagcgtct gcccatcctc ctccgcccat agcgcgcata
      901 gcaggtgtcc catcggtact actccttccc gagggctgtc ggaggtccgg gagttagggg
      961 cactcctgag agtggtgacg agccgcgaag gccggtggaa ctcatcggga atcgtgccca
     1021 tatgggaatc aggggcgcgt tgcccatgcc gggcagcgcg cccccggtgc tctcaggtta
     1081 cgaacacccc tattcagacc gtcagaggtg attgccgacg gagctgtagt tcgtttgcac
     1141 cttgccgctg aggtaggaca tcgcggcgat cgccgcaacg gcgatgaggg caatgatgag
     1201 agcgtattca accagcgttt gcccttcttc ctccacccac agcttgcaca gcagagactt
     1261 catgtgcatc tccttccacg agggcactcg ccgatggtcg gcgggctgtc atgcgtgtta
     1321 gagctcgttt ccgacggagc tgtagttcgt ttgcaccttg ccgctgaggt aggacatcgc
     1381 ggcgatcgcc gcaacggcga tgagggcaat gatgagagcg tattcaacca gcgtttgccc
     1441 ttcttcctcc acccacagct tgcacagcag agacttcatg tgcatctcct tccacgaggg
     1501 cctctaccca tgattctggc aagcgcccat agagtgcgca tgggaagggc ggcccaggcg
     1561 atctgggcca atagtcctgc cacatctcat tctcggacgg gcagggcccc ttttggcccg
     1621 ccgatccggc ggaaggaatc gaagccggtg gcaacagaat gaagtggata agatggaaac
     1681 gttcttttgt gactggcgtc tccgtgccgc aggcgtgaag tgcatctgct cattagggca
     1741 acggttggag tggctcgcat gaagccccgg cgcgaagcac tctcgctcga gacccgccgt
     1801 ttcctgagcc tggacaacgt ctacctcgcc gcggcggtgg cggtcatcat gatcgtgccg
     1861 ctcctcaccc gcgttgcgcc ccacgacttt tggtggcacg tggcgatggg ccggatcatc
     1921 gttcgcgagg ggtggatccc cacggtcgat tccttctcct tcacgcgcgc aggtgagccg
     1981 tacttcaatc aggggtggct ggcgcagtgc gtgatgtacg cgcttcacca ggcgggcggg
     2041 ctgccgctgc tcgtggtggt ccaggcggtg ctaatggcag tggcctacct gttgctcctc
     2101 tggatggtcg tccggaagac ggggcggctc cgcctggccg cggcggtgct tctcctggcc
     2161 atcgagccga acgcctacac taactggagc atccgcccgc agagctacgc catcccgctc
     2221 ttcgccagct tcctggtgat cctgaccggc taccgtcaac ggtggtggcg cactctctgg
     2281 ccgctggtac cgatgatggc cgtgtgggtg aacctgcatg gctccttccc gctggggttg
     2341 ggcttgatgg gcctggtgct gctgggggag gggatccgtc gcaaggcagg agattccggc
     2401 gccctcccgg cgcgcgagtg gtggcggctg gccggctata ccgtggccgt ggcgctggca
     2461 actctcgcca accccaccgg tccgcgcgtg ctgggctatg tgagccacct gatgaccgcg
     2521 aagagcgtgg tgaacctcgt gaccgaatgg gcgcccccga cacccagaga cgcggctggc
     2581 gcgatcttct tcgcgctggt gatcgctctc ttcgtggcgc tgatctacgc gcgccggcgc
     2641 ccggcgctca ccgatctctt cctcatgctg gcgtttctct ggctggggct ctcggccggg
     2701 cgcaacatcg tgtggtttgc gatggtggcc tatctcccgc tcgccgaggc cctggcgtcg
     2761 cactggagtg acgcgccgcc ggccagcaag cgggatggcg cgggacaggg gatccccgcg
     2821 gccaacgccg tgctagtcag cgcgctggcg accgcggtcg ttctgtcgct gccgtggatc
     2881 cgccccctcc tggggcgtcc ggtgtcgttg ctcacggagg acacgccggt caaggcggtg
     2941 gagaagctgc gcgcgctccc gccggaagaa cgccccaagc ggctgttcca caccacgggc
     3001 ttcgggagct acctgatctg ggcggcgccg gagcaaccgg tcttcatcga tccccgcttc
     3061 gagttctatc cggtggacca gatcaacgac tgccggtttg ccagcgcggg atacaatgtc
     3121 gaggagaccc tggccaggta tcggatcgac gggttgctgc tggaccggaa aagccaggag
     3181 aaactggagg agcgcgcgcg ggcttccggg cggtggtgcg aggtgtaccg cgatgctaca
     3241 gcagtgctga tggtgccagc taggcccgcg ccctagcggg cgaggtgccg cccgaggaac
     3301 caaagctcag gggcgcgctg cgcgcgcccc tgaatcgctg aaaaggatgt tcttggcgag
     3361 tgctcctttg ccactgactc ctgcgccgtt cctctgcagg gcagagcggg ctaactaagg
     3421 gtgcaatcct aactgcccgc aggaagagca ctctggtgat gcatcagagc ttgtcaccga
     3481 ccgagctata gttcgactga accttaccgc ttaggtaagt catcgcgcta acagcagcga
     3541 ctgcaatcaa cgcgatgatc aaagcgtatt cgaccagcgt ttggccttct tcctcagccc
     3601 agagctttcg gagcatcgac ttcattcgtt ttcttctccc tccagcagta gcctgctggt
     3661 gtatattatc tcattcctga gcggatgagg taatgggtta ttggacccag cgctcctagg
     3721 ccaatagtcc tatgcccacg ggcataagga tcacgcgggc gtaggcagca cggatataaa
     3781 cgtccgcact gcgtgtgggc gaagccctgc gagctgctgg ggctatgttc gccggcaatc
     3841 taggcaggcg ggcgcggggg cggcggaggc ccgtaggacc tttgctggga tgggaaggcc
     3901 cgcccttacc catccgcgtc ggtcctccat ccgtggtatc cccggcccgc gcgcctgtcg
     3961 gcgcgcgccg cagtgaaggc ttggcgagtg caggcaggca tggtatagta tagttggcgc
     4021 caggcgggac ggaggcgcgc cggttggtca gatcctcaag cgccgtgcgg agggctcgtg
     4081 gtgtgaaagc aaaggcaggt gaggtgaggc agcagtgtct ggcgcgcgtg gggaaaatca
     4141 ccgcgctcgc tctcgtgttg gccggtctcg tttggcaagg gacgcgcacc ccggcactcg
     4201 ttgatccctc ggcaggagac tacgaagcct attggatggc agcgcgcctg cttctcacag
     4261 gaggcaaccc ctacgatctg gatgcggttg ccgccttgca gggcgtgcca cccctccaaa
     4321 acggaagcgt gaaggtcgcc tggaatcccc cctgggctct ggcggtgatg cttcctttcg
     4381 ggctcgtgga atttccgttg agccgcatgc tttggtttct cctcatggtg gcggttctct
     4441 tcgcgagcac gagcgttttc tggctacgcg acggtggccc gctctcgcgg cgctgggtag
     4501 cggctctcct gtgcatcctc ttcccgccag cgctcctggc gctgtggaac ggtcagatga
     4561 cgcccctgtt gttggcgggg atcgcgggct tcctccacag cggcgcgcag ggccgatggg
     4621 ggcgcgccgg cgcgttcctt gctctggggg cactc
//
LOCUS       DAOSCV010000262         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11646_length_4654_cov_3.193520, whole genome shotgun sequence.
ACCESSION   DAOSCV010000262 DAOSCV010000000
VERSION     DAOSCV010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11646_length_4654_cov_3.193520"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLY56_07765"
     CDS             complement(<1..1155)
                     /locus_tag="PLY56_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ21416.1"
                     /translation="MLAALLLLVAGGTAEPARDARRETPRIPIVLFDLVCEAPPPDEA
                     PDANWLTRSLTEALIEALKARGYDPMRFNPESPSIIRAVDRERILTLEDTAHPEREAS
                     ARKIAAAYGARYVLFPKLMELTLDLPGAQARLRLGLRVIPDIGATREVTGDGMAQLKP
                     EKRAKKLAERILAQAGQAAAEELVQSLSAMASGGPGSATAPREAETYYAQAMQSLKEN
                     RLELVIPLLERATLLDPQNAKYAVALGESYQQKGQMANALMEFRRAVTIEPGNIRHRV
                     RVAKILLERKLEREALSELRRAVTLAPEDEEARAALLEAYQRSGMVEEALEEARRMVQ
                     ARPKDAGLRVTLGDLLARQGAGDDAIREYQEAVTLDPESPVPHERLAALYR"
     gene            complement(1437..2747)
                     /locus_tag="PLY56_07770"
     CDS             complement(1437..2747)
                     /locus_tag="PLY56_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ21417.1"
                     /translation="MQAPSPAPPVDHGKVAVLMEAAFSCHREGKIDEAIEKCLQATAL
                     NDQSTSAHSLLALLYEEKGQLDLAVVHLRRVLELNPDSVADQEKLARLLGEAPLQDAT
                     ADEPNPPSWAADLLSRPLALSIGAGVLVMALVLAVSIPRLSSPAGRGRTEVARRPAPT
                     TPPPAYSTGGAVRNSAPMGPSVAPPSNPQVSQPAQGAPAPAMVPPRADESAPLAQERE
                     MRSRTAQPPAPPSPSPTPTPVLMPEDEIEPLPPPAPRRVASPPVIYLPPTRRPAMETP
                     PAQEPPPSAVTAPPAARAQGPVEVAVTIEQGTPTPTRAEQGTPRAPSPPAPAPKPPAR
                     AREASQTDYQERGLAASARGDYDEALAHFRKALEGARTSRERGKIHQHMAYAYQQLGR
                     SNDAGMEYRRAISAYEEQLREGTNTDLARSSIRSCQDGLRMLGM"
     gene            complement(3027..4436)
                     /locus_tag="PLY56_07775"
     CDS             complement(3027..4436)
                     /locus_tag="PLY56_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21418.1"
                     /translation="MTFLLGGAVVLFASALALTPFESHDLWWHLRAGDEILRAGRPPT
                     RNLFSFTAPDHPWITHEWLSEVLFAWVYGQIGPGALVVLKVLVIAATFLVVYGSAWRE
                     SRNAWAAVATTALAAFAARFTYDIRPQLFTYLFLGLCLAAIARHRQRGDRGLLLLVPL
                     FCVWANMHSGFIVGLILLALAAATMPRHARAFLGTLALATAAALVTPFHWRGLWFPVE
                     VSRTRLFTDTLTEWFSPDFHSPWLFGFEVLLLVCIGVLASSPARPTLFELGTLLGMAH
                     LALQHQRHVPLFAVAVAPILARHLACLAERVAERPVIPSRWAPLAAGAATASALVFLC
                     LAVPRANAFDQVTRRDTFPTAAAEFLRRQPPLRLYNSYIWGGYLIRSCPEQPVFIDGR
                     ADAYPHQVLRDYLAIERLQPGWRELLVNYRVGAVVYQPGTPLADALQEDPEWRVAYAD
                     AVAVVFLRRTNSWDDAGTP"
     gene            complement(4475..4627)
                     /locus_tag="PLY56_07780"
     CDS             complement(4475..4627)
                     /locus_tag="PLY56_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ21419.1"
                     /translation="MFHLGMPELLILLAIILLLFGAKRLPELARGVGKSITEFRKAAN
                     GEEKEA"
BASE COUNT          782 a         1594 c         1561 g          717 t
ORIGIN      
        1 ccggtaaagc gccgccagac gctcatgcgg caccgggctc tcggggtcga gcgtgaccgc
       61 ctcctgatac tcccggatcg catcatcccc ggccccctgg cgtgccagca gatcccccag
      121 ggtcacccgc agtccggcat ccttcggacg ggcctgcacc atccgcctgg cttcctcgag
      181 cgcttcctcc accattccac tgcgctggta ggcctccaac agggcggcgc gcgcttcctc
      241 gtcctccggg gcaagcgtca ctgcgcggcg caactccgaa agcgcctcgc gctctagctt
      301 ccgttccagc aatatcttcg ccactcgcac ccggtgcctg atgttgcctg gctcaatcgt
      361 cacagcgcgc cggaactcca tcagggcatt ggccatctgc cccttctgct gatagctctc
      421 gcccagcgcc accgcatatt tggcgttctg aggatcgagc agcgtcgccc gctccagcag
      481 cgggatcacc agctccagac ggttttcctt caggctctgc atcgcctgcg cgtagtacgt
      541 ctccgcctct cggggggcag tggcggaccc aggcccgccc gacgccatcg ccgacaggct
      601 ctggaccagc tcttcggcag ccgcctgtcc ggcctgcgcg agaatccgct ccgccagctt
      661 cttggcgcgc ttctccggct tgagctgcgc catgccatct cccgtcacct cgcgcgtcgc
      721 gccgatatcg ggaatgacgc gcaggccaag ccgcaggcgg gcttgtgccc cgggcagatc
      781 cagggtgagc tccatcagct tcggaaagag gacgtagcgc gcgccgtatg ccgcggcgat
      841 cttgcgcgcc gatgcctcgc gctccggatg cgcggtatcc tccagcgtga ggatgcgctc
      901 gcggtcgacc gcgcggatga tcgagggcga ttccgggttg aaccgcatcg ggtcatagcc
      961 acgcgctttc agcgcctcga tcagcgcctc ggtcaggctc cgcgtcagcc agttggcatc
     1021 aggagcctcg tcggggggcg gcgcctcgca caccaggtcg aagaggacta tcgggatgcg
     1081 aggagtttcc cggcgggcgt cacgagccgg ctcggcggtt ccacccgcga caagaagcag
     1141 gagtgccgcg agcatcaggg gcacgctctg aagcccacgg cggaacgcgc gcgcaggggc
     1201 cgccggccag gccggcgaag acgcagctag tcgcgcgcct gcgcgtattg cggatctcca
     1261 caaggtgcgc attacactcc tctcccctgc acggcgatac tcccaccagg agcgatccgg
     1321 cttctggaag ccataggttt ctggatcgat agtgcgggaa tcgctccacg aaggtcctgg
     1381 cgaggcaggc ggaccgcctt tccggcggca atcacgagcg aggtggcggg gcgccgtcac
     1441 atgcccagca tgcgcaggcc atcctggcac gagcggatgc tggaacgcgc cagatcggtg
     1501 ttggttccct cgcgaagctg ctcctcgtag gcggagatag cccggcgata ctccatcccc
     1561 gcgtcgttgc tacgccccag ctgctggtag gcataggcca tatgctggtg gatcttgccc
     1621 cgctcgcgcg aggtgcgcgc cccctccaat gccttccgga aatgggcgag cgcctcatca
     1681 taatcgcccc gcgcagatgc cgcgagcccg cgctcttgat agtcggtctg cgaagcctcg
     1741 cgcgcccgcg cgggcggctt cggcgccggc gccggaggcg aaggagcgcg cggcgtgccc
     1801 tgttcggcgc gcgtcggcgt tggagttcct tgctcgatgg tcaccgccac ctccaccgga
     1861 ccctgcgcgc gtgccgccgg gggcgccgtc accgcgctcg gaggcggctc ctgcgccgga
     1921 ggcgtctcca tagccggcct ccgcgtcggt ggcaggtaga tcacgggagg cgaggccacg
     1981 cgccgaggcg cgggtggcgg cagcggctcg atctcatcct cgggcatcaa cactggggtc
     2041 ggggtcggcg acggcgatgg cggtgccggc ggctgtgccg tacgcgagcg catctcgcgc
     2101 tcctgcgcca ggggtgcgct ctcatcagcg cgcgggggta ccatcgccgg agccggagct
     2161 ccctgcgccg gctgagacac ctgcggattc gatggcgggg cgacgctcgg ccccatcggc
     2221 gcgctgttcc gaaccgcgcc cccggtgctg taggccgggg ggggagttgt cggcgccggc
     2281 cggcgcgcca cttccgtgcg cccacgcccg gcaggactgc tcaggcgcgg aatcgagacc
     2341 gcaagcacca atgccataac caagacgccc gcgccgatgc tcagcgccag cgggcggctg
     2401 agaagatccg cggcccacga cggggggttc ggctcatcgg cagtcgcatc ctggagcggg
     2461 gcttcaccca gtaaccgcgc cagtttctcc tgatcggcca ccgaatcggg gttcagttcc
     2521 agcacgcgcc ggagatgcac caccgccagg tcgagctggc ccttctcctc atagaggagc
     2581 gccaacagcg agtgcgccga cgtgctctgg tcgttgaggg cagtcgcctg aaggcacttc
     2641 tcaatcgcct cgtcgatttt tccctcgcgg tgacaggaga aggccgcctc cataaggaca
     2701 gcgaccttgc cgtgatcgac cggtggcgcg ggactaggtg cctgcaaatc ggcgaactgt
     2761 tcctcactga gcgtccgggg agcccgttcg ggcagcctgg tgccacactc gcgacaaaat
     2821 ttgttgatat ccgggttctt ggtcccgcaa tggccgcaga acacggcgac ctcctttgcc
     2881 ggcagccggg tcgcagtctg ggggctcctt gcaccgcacg acagcgcgcg aacgccgacc
     2941 cgaccgcccc acgccggctg gcgttccgac cccgtcgtag taacgctgtt tctggtacag
     3001 attatatcgc gcgccccaac ccggcgtcaa ggcgtgcccg catcatccca agaattcgtt
     3061 cggcgcagga agaccacggc caccgcatcg gcgtaggcca cccgccactc aggatcctct
     3121 tgcagcgcat ccgccagggg cgttcccggt tgatagacga ccgcacccac gcggtagttc
     3181 accagaagtt ctcgccaccc tggctgaagc cgctcaatgg ccaggtaatc gcggagcacc
     3241 tgatgcgggt aggcatcggc gcggccatca atgaaaaccg gctgctccgg gcaagagcgg
     3301 atgaggtagc ccccccagat gtaggagttg tagaggcgga gcggcggctg gcgccgcaga
     3361 aactcggccg cggcggtggg gaacgtatcg cgccgcgtca cctgatcgaa ggcgttggcg
     3421 cgaggcacgg ccaggcatag gaacactagc gcgctcgcag tggcggcgcc ggcggcgagt
     3481 ggggcccatc gggatgggat cacagggcgt tcggccactc tttcggctag gcacgctaaa
     3541 tgccgcgcca ggatgggcgc aaccgccacg gcaaagagcg gcacatgccg ttggtgctgc
     3601 aacgccaggt gcgccatccc caggagggtg cccagctcga agagcgtggg ccgtgccggc
     3661 gaactcgcga gcaccccaat gcagaccagg aggagcacct cgaagccaaa cagccacggg
     3721 gagtggaagt ccggtgagaa ccactccgtg agcgtatcgg tgaagagacg ggtgcggctc
     3781 acctccaccg gaaaccacag cccgcgccag tggaatgggg tcaccaaagc ggcggcggtt
     3841 gccagcgcga gcgttcccaa gaaggcacgc gcatgccggg gcatcgtcgc cgcagcgagc
     3901 gccaggagga tcaggcccac gatgaacccg gaatgcatgt tggcccacac gcagaagagt
     3961 gggacgagga ggagcaggcc ccggtcgcct ctctgtcgat ggcgggcgat cgccgctagg
     4021 cagaggccga ggaagaggta ggtaaagagc tgggggcgga tgtcgtaggt aaagcgggcg
     4081 gcgaacgcag cgagtgccgt cgtggccacc gcggcccacg cgttgcggct ttcgcgccac
     4141 gcgctcccgt agacgacaag aaacgtcgcc gcgatcacca ggaccttgag caccaccagg
     4201 gcaccgggac cgatctgccc gtacacccac gcgaagagaa cctccgagag ccactcgtgg
     4261 gtgatccagg ggtggtctgg cgcggtgaag gagaaaaggt tgcgcgttgg cgggcggccg
     4321 gcccgcagaa tctcatcccc cgcccgcaga tgccaccaca ggtcgtggct ctcgaacggg
     4381 gtgagcgcca gcgcggacgc aaacagcacg acagcgccgc cgagaagaaa ggtcacggcc
     4441 cgcttcgcgc ggtgtggaga cacggtggcc ggcttcaggc ttccttctct tcgccgtttg
     4501 cagccttgcg gaactccgtg atgctcttgc caacgccccg ggcgagttcc ggcagacgct
     4561 tcgctccaaa gaggagcagg atgatcgcaa ggaggatcag aagctctggc atgccaagat
     4621 gaaacattac gacctctcct tctggatgct gtta
//
LOCUS       DAOSCV010000263         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11648_length_4654_cov_2.692977, whole genome shotgun sequence.
ACCESSION   DAOSCV010000263 DAOSCV010000000
VERSION     DAOSCV010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11648_length_4654_cov_2.692977"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2482)
                     /locus_tag="PLY56_07785"
     CDS             complement(<1..2482)
                     /locus_tag="PLY56_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21420.1"
                     /translation="MRPRTPVSVACLALALLASSAPMARDEITLLDGPIVIFPGQSFR
                     VALEQPAGSGDLDAAVPPTLELFDRWPKDSIQRFYFRALAPGDATLRFHGQAGELTLS
                     LKVTPWSEVLQPRQWNKIDFPRIWPMAEPGLRELKTRRTLHSPEEIAAIKAGGKPGAI
                     ARKWHALSDEEIWNIIPGACVPRTCLMVLSPFEDARGKGCPVCGPKIYEGRNAFYPWR
                     IDPEGHPWKVGCPSCNTWFPSNDFAKGDMHSGDFPDDGYGCEPARPVISPNGKPWRWP
                     FIAYYHQWAAYMDHFSQGIPQCAEASIITGDRTYARKAAIALFRISESLLDLAVNMNH
                     RKIPVRNGVYQPPIGAPDAKAMARVAGSFLYIQPNWDTPRLESYARAWDLIFDQLEGD
                     EALLEFCRAHHHPEIRTLEDFRWFIESGILRVAAQACLDDAVSRNYPMQEATVATLAL
                     ALGTPRASELADWVINGPGGIRFGLSNEFFKDGSGHESESYNAIGIRDMERLFLTLER
                     LYALDPERYRPPRFPSLLKDPKYPLLFHFPLANSLIGRTYPSTGDTGGPRTAPSAPSQ
                     GYPLSQAQFVEVYRRTRDPRFAQALYGPAGEIPSALHEPELRAEVERIGKERGWQVQT
                     KSNFLDGYGHAILRSGTGDRQRALWLRYGRALKHLHYDMLTFGLSALQRNLLPELGYP
                     QGWTYASVWEFSWGTHYGTHITGVATSAFPRGRLTLFADSPPAQVAVAEASTGPGKDD
                     AWRQRTLVLVDLPGENCYAVTLERVRGGQEHLHSFHGPDGEATLFGLSPKPQGGGTVL
                     GTDIAYGDVSKAGHDRELAILAYLPNPSR"
     gene            2617..3375
                     /gene="minC"
                     /locus_tag="PLY56_07790"
     CDS             2617..3375
                     /gene="minC"
                     /locus_tag="PLY56_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOJ21421.1"
                     /translation="MDPTKTREPVSVKGIRDGFLILLDEDGEFGGVLGHLREKLSGAA
                     EFFRGAGAIVDIGRRELSREEFARLIHTLQDEYGLVVRRVVSNLPERNRPGGVEGAVV
                     LQQWSVAEEGDPEPERPVSAAVLPSTRDQSGQRQQQIEERTLFLHRTLRSGQRVQYDG
                     SVVIIGDVNAGAEVVASGDILVMGALRGLAHAGARGAANAIVSALCLAPTQLRIANVI
                     GRPPDGDLARPRPEVASVRDGMLLIEPYPGWEEH"
     gene            3552..4355
                     /gene="minD"
                     /locus_tag="PLY56_07795"
     CDS             3552..4355
                     /gene="minD"
                     /locus_tag="PLY56_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOJ21422.1"
                     /translation="MGEVVVITSGKGGVGKTTTVANLGVGLTALAGKKVALVDTDIGL
                     RNLDVVMGLENRIVYDLVDVAEGTCKLRQALIKDKRYDGLFLLPAAQTRDKNAVSPDQ
                     MRKICQDLRSEFDFVLIDCPAGIEQGFRNAVAGAQKAVVVTTPEVSAVRDADRIIGLM
                     EAEELRDIRLIVNRVRAHMVKRGDMMDINDISEILSVPILGVVPEDEAIVVSTNRGEP
                     AVMDPKAGASRAYKNITRRLVGEDVPLMSLEEDDGMLGRIRRFLGIGKA"
     gene            4373..4636
                     /gene="minE"
                     /locus_tag="PLY56_07800"
     CDS             4373..4636
                     /gene="minE"
                     /locus_tag="PLY56_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOJ21423.1"
                     /translation="MPFLSRLFGRPEPSGKVASERIRRVLVQDRASVSPQTMERMRAN
                     LTRVLSEFMDVDEQNSVITITDTGGAVSLVATVPIRRMKRGKR"
BASE COUNT          807 a         1360 c         1678 g          809 t
ORIGIN      
        1 aacgcgaggg gttgggcagg taggcgagga tggccagctc gcggtcatgc cccgccttgg
       61 acacatcgcc atacgcgata tcagtgccga gcaccgtgcc gccaccctgc ggcttgggcg
      121 acagtccgaa gagagtggct tcaccatccg gcccgtggaa tgagtggaga tgctcctggc
      181 cgccgcggac gcgctcgaga gtgaccgcgt agcagttctc tcccggaaga tcgacgagga
      241 ccagggtgcg ctggcgccag gcgtcgtctt tcccaggccc ggtggaggcc tcggcaaccg
      301 cgacctgcgc cggaggcgaa tcggcgaaga gcgtcagccg accgcgcggg aaggcgctgg
      361 tggccacgcc cgtgatgtgg gtgccataat gggtgcccca gctgaactcc cacacgctgg
      421 cgtaggtcca cccctgggga tagcccagct ccgggagcag gttgcgctgc agggcagaga
      481 ggccgaaggt gagcatgtcg tagtggaggt gcttgagcgc gcgcccgtag cgcagccaga
      541 gggcgcgctg ccggtcgccc gtgcccgagc gaaggatggc gtgcccatag ccgtccagga
      601 agttgctctt cgtttgcacc tgccagccgc gttccttgcc aatccgttcc acctcggcgc
      661 gtagctccgg ctcgtggagc gcgctgggaa tctcacccgc cggcccatag agtgcctgcg
      721 caaaccgggg atcgcgcgtg cgccggtaga cctccacgaa ttgcgcttgc gagagcgggt
      781 agccttgcga gggcgcggaa ggagcggtgc gcggtccacc ggtgtcgccg gtgctggggt
      841 aggtgcgccc gatcaggcta tttgccagcg ggaagtggaa gaggagcggg tacttcgggt
      901 ctttcaggag cgaggggaag cgtggcgggc ggtagcgctc cgggtccagg gcgtagagcc
      961 gctccagcgt caggaagagg cgctccatgt cacgaatccc gatggcgttg tagctctccg
     1021 attcatgccc ggagccatcc ttgaagaact cgttgctcag cccgaagcgg atgccgcccg
     1081 ggccgttaat cacccaatcg gccagctcgc tggcccgggg cgtgcccagc gccagggcta
     1141 aggtggccac cgtggcctcc tgcatcgggt agtttcgaga gacggcgtca tccaggcacg
     1201 cctgcgccgc cacgcggagg atcccgctct cgatgaacca ccggaagtcc tccagggtgc
     1261 ggatctccgg gtggtggtgc gcccggcaga actcgaggag cgcctcgtcg ccttcgagct
     1321 ggtcgaagat caggtcccag gcacgcgcat acgattccag gcggggagtg tcccagtttg
     1381 gctggatata gaggaaggac ccagctaccc gggccatcgc cttcgcgtct ggcgcgccga
     1441 tggggggctg gtagacgccg ttgcgcacgg ggatcttgcg gtggttcatg ttcacggcga
     1501 gatcgaggag gctctcggag atgcggaaga gggcgatggc ggccttgcgc gcgtaggtgc
     1561 ggtcgccggt gatgatgctc gcctcggcgc actggggaat cccctgggag aagtggtcca
     1621 tgtaggcggc ccactggtgg tagtaggcga tgaaaggcca gcgccacggc ttgccgttgg
     1681 gcgagatgac cgggcgggcc ggctcgcagc cgtagccgtc gtcgggaaag tcgcccgagt
     1741 gcatgtcgcc cttggcgaag tcgttggagg ggaaccaggt gttgcacgag gggcagccca
     1801 ccttccaggg atggccctcg ggatcgatgc gccaggggta gaaggcgttt cggccttcat
     1861 aaatcttcgg gccgcacacc gggcagcctt taccacgcgc gtcctcgaaa gggctgagga
     1921 ccatcagaca ggtgcgcggc acgcacgcgc ctgggatgat gttccagatc tcctcatcag
     1981 aaagggcatg ccacttgcgc gcgatggccc caggctttcc gcctgccttg atcgccgcga
     2041 tctcttccgg cgagtggagg gtccggcgag tcttgagctc gcgcaggccc ggctcggcca
     2101 tcggccagat gcgtgggaag tcgatcttgt tccactggcg cggttggagc acctcgctcc
     2161 agggcgtcac cttcagcgaa agggtcaact cgcccgcttg cccgtggaag cggagggtgg
     2221 cgtcaccagg tgcgagcgcg cgaaagtaga atcgctggat ggaatccttg ggccagcggt
     2281 cgaatagctc cagggtgggc gggaccgccg catccagatc gcctgatccg gccggctgct
     2341 ccagggcaac gcggaagctc tgcccgggga agatgacgat ggggccatcg agaagcgtga
     2401 tctcgtcgcg ggccattggg gccgagcttg cgaggagagc caaggccagg caggctaccg
     2461 aaacgggcgt gcgagggcgc atatcgggct cctttctggc actggtttcg cggcggtaga
     2521 gacgcttcct cctctcgatc tctctctgct ggcacgcagg aatcggtggc caagcgcgga
     2581 atgatcacaa cggtaggttg ggagggctag ccaaacttgg atcccacaaa gacacgagag
     2641 ccggtttccg tgaagggaat ccgggatggt tttctgatcc tgttggatga ggacggcgag
     2701 tttggaggag tgctcggcca cctccgcgag aagctgagcg gcgccgcgga gttctttcgg
     2761 ggtgctggcg ctattgttga tatcggtcgg agggagctga gcagggagga gtttgcccgg
     2821 ctcatccata cgctccagga cgagtatggc ctggtggtgc gccgcgtggt ctccaacctg
     2881 cccgagcgca accgccctgg cggcgtcgag ggtgccgtgg tgctccagca gtggagcgtc
     2941 gcggaggagg gagaccccga gccggagagg cctgtatctg ccgcggtgtt gccgagtacg
     3001 cgggatcaga gcggccagag acagcagcag attgaagagc gcacgctctt tctccatcgc
     3061 accctgcgca gcgggcagcg ggtccagtac gatggcagcg tggtgatcat tggcgatgtc
     3121 aacgccggcg ctgaagtcgt ggcgagtggc gacattctgg tgatgggcgc actgcgtggc
     3181 ctggcgcatg ccggcgcgcg tggcgccgcc aacgccatcg tttcggcact ctgcctggcg
     3241 ccgacacaac tgcgcatcgc gaatgtgatc ggccggcccc cagatggtga tctcgcccgg
     3301 ccacgccctg aggtggcgag cgtgcgcgac ggcatgttac tcattgagcc ctatcctggc
     3361 tgggaagagc attgatcgtt gagctcaggg ttcagcgggg cgggctgacc tgtgattggt
     3421 aagggcgaca agtagcactt aggatcctga accagaggcg ggcgagcgcc accggttgaa
     3481 tgcgagcggc ctcggcgccc gatacccatc ccccggatcc ggaccctgta gaccaagaag
     3541 gagcataccc tgtgggtgaa gtagtggtga tcacatccgg taagggcggc gttggcaaga
     3601 ccaccactgt cgccaacctg ggcgtgggcc tgaccgctct ggcaggcaag aaggtcgccc
     3661 tggtagatac cgatatcggc ctgcgcaact tggacgtcgt gatgggcctg gagaaccgca
     3721 tcgtctatga cctggtggat gtggcggagg gcacgtgcaa gctgaggcag gcgctgatca
     3781 aagataagcg ctacgatggc ctctttctgc ttccggccgc tcagacgcgc gacaagaacg
     3841 cggtgagccc ggatcagatg cgcaagatct gccaggacct gcgcagcgag ttcgatttcg
     3901 tgctgattga ttgcccggcc gggatcgagc agggttttcg caacgccgtt gccggggcgc
     3961 aaaaggcggt ggtggtgacg acccctgaag tctcggcagt gcgggatgcg gaccggatta
     4021 tcggcctgat ggaggccgag gaactgcgcg atattcgcct gattgtaaac cgcgtgcgcg
     4081 ctcacatggt gaagcgcggc gatatgatgg atatcaacga catcagcgag atcctctcgg
     4141 tgccgattct tggcgtggtg ccagaggatg aggcgatcgt cgtctcgacc aaccgtggcg
     4201 agccggccgt catggatccg aaagccggtg catcgcgggc ctacaagaac atcactcgcc
     4261 ggttggtggg cgaggatgtt cccctcatgt cgctggagga ggacgacggc atgctcggcc
     4321 ggatccggcg gtttttgggt attggcaagg cataggtagt aagggggaga ccatgccttt
     4381 tctcagtcgc ctttttggcc ggcccgagcc ctccggtaag gttgccagcg agcgcatccg
     4441 ccgggtgttg gttcaggatc gagccagtgt gtcgccgcaa acgatggaac gcatgcgcgc
     4501 caacctgacg cgggtgcttt ccgagttcat ggacgttgac gagcagaatt cggtgatcac
     4561 gatcaccgac acgggagggg ccgtcagcct ggtggcgacc gtgcccatcc gcaggatgaa
     4621 gcgcgggaag cgctgagcga gggcgcgcgc gtcg
//
LOCUS       DAOSCV010000264         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11651_length_4653_cov_3.541105, whole genome shotgun sequence.
ACCESSION   DAOSCV010000264 DAOSCV010000000
VERSION     DAOSCV010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11651_length_4653_cov_3.541105"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLY56_07805"
     CDS             <1..1029
                     /locus_tag="PLY56_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ21424.1"
                     /translation="FWAGLSGLLVLWIGGMEVLHGRLTLPEYVAFEAYLLLLLLPMRM
                     LGYMVSGMQRAAAAINRLTAILDEVPDVRDPERPVRLGRIEGAVRFENVTFGYTRDTP
                     VLRGVSFSVRPGETVGILGATGSGKSTLVALIPRFYDPQEGVVRIDGHDVRALDRREL
                     RRQIGIVFQESFLFTGTIRENIAFGHPDATDAEVEEAARKADIHDFVASLELGYQTPV
                     GERGLTLSGGQQQRVALARALLTDPRILILDDCTSSLDTRTEHRIQTRLASYMAGRTT
                     FIIAQRASSVARADRIFVLENGAIVEEGAPDDLLRNPESLFARLYQLQEQAGSRAGES
                     GLPAPASG"
     gene            1062..1700
                     /locus_tag="PLY56_07810"
     CDS             1062..1700
                     /locus_tag="PLY56_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HOJ21425.1"
                     /translation="MTEAHHPSLDTGLIESLTDLERAREIAAGCVRCPLKETRTLVVF
                     GEGNPGASLMLVGEGPGEEEDARGRPFVGRAGQLLDSALAEAGIPRDAVWVTNTVRCR
                     PTKIEAGRVANRPPRVGEIRACAVWMETELRLVAPRVVVCLGAVAAKALIGKEFRLTQ
                     ERGQLFPGPAGTEAVATLHPSYIIRQRDSESRARAREMLVEDLRLAIRRAGA"
     gene            1852..2982
                     /locus_tag="PLY56_07815"
     CDS             1852..2982
                     /locus_tag="PLY56_07815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21426.1"
                     /translation="MMTGPRACAPSELPQLARLADAVFRSKGGSMQAEYPLVFGEENL
                     NFCRVIEEDGRIVSHVGVSIRDASLLGCSLRVASIGAVCTDAAYRGRGYASLLMEDAR
                     TLALGEGASLMLISGGRGLYHRLGYVNVGSFRSFTARAEILPVPAEGLRVEPCGAAQI
                     PGLIALHQRLPVRFVRPADDWEKLLAAGMIMNQRGDCLGVWEEGRLVAYAAVQRPRDG
                     APARVREYAGSETALFAALGAVARYYEASGVEVVAMPGTEDLARLLRNAGAEEKELAF
                     TGTLGILDVDGFFACIHPYVRECLGAPTDALFLDPRQTNSISFEYAGERYALANRGQL
                     TALVFGGSTEEARSIPEAPPGVGEILKRIFPMPLLWYGYNYV"
     gene            complement(3136..4128)
                     /locus_tag="PLY56_07820"
     CDS             complement(3136..4128)
                     /locus_tag="PLY56_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002645970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ21427.1"
                     /translation="MSAPQVILSGFADEGSKRAEEQFTMLRALGMSYYSIRFVDVGEG
                     VKNVMKLSDAEIKRLCELHDQFEVQVSSIGSPIGKIKLVDQEDGTHNVYMPIDEYLEK
                     EVPRAISLAHSFGTKLIRGFSFYPPKDADPWQYVDQAADYVGRVVEKCKAEGVFYGLE
                     VEANLVGRNGTLLRAIRDRINNDHLYLIFDGANIVCQGYDTEETYHEYTAMKPYIGWM
                     HIKDYERAPGEEWQGYVNEEQLNRMVPADQGDSGHERILRDFKDRLPELTAELQSRGV
                     PGVFLDLEPHVKGGGQFGGYSGPDGFGAALRSLCRVLDYVGIGYHLTGYGEWRK"
     gene            complement(4333..>4653)
                     /locus_tag="PLY56_07825"
     CDS             complement(4333..>4653)
                     /locus_tag="PLY56_07825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ21428.1"
                     /translation="RPGYTGKGDIATLPSLALFLGLASTVLMPAGNALSRVWERQCDR
                     FALDMTRQPDAFIGVMRKLAAQNLADPAPSALIEALLYTHPSIGNRIRMALRWEALNA
                     ERAE"
BASE COUNT          725 a         1506 c         1591 g          831 t
ORIGIN      
        1 ttctgggccg ggctgagcgg cctgctcgtt ctctggatcg gcgggatgga ggtcctgcac
       61 ggccggctca cgcttccgga atatgtcgcg ttcgaggctt acctgctgct gctcctcctc
      121 ccgatgcgca tgctcggcta catggtctcc gggatgcagc gcgcggctgc ggcgatcaac
      181 cggctcaccg ccatcctcga cgaagttccc gatgtgcgcg acccggagcg tccggtccgc
      241 ctgggccgca tcgagggcgc cgtccgcttc gagaacgtca cctttggcta cacccgcgac
      301 acgcccgtcc tgcgtggcgt gagcttcagc gtccgcccgg gcgagacggt tggcatcctg
      361 ggcgcgaccg gctccggcaa gagcacgctc gtggcgctta tcccgcgctt ctacgacccg
      421 caagaaggcg tggtccgcat cgatggccat gacgtgcgcg cgctcgaccg gcgcgagttg
      481 cgccggcaga tcgggatcgt cttccaggag agcttcctct tcaccggcac catccgcgag
      541 aatatcgcct tcggccaccc ggatgcgacg gacgcggagg tggaagaggc cgcccggaag
      601 gctgatatcc atgacttcgt cgcatccctg gagcttggct accagacgcc cgttggcgag
      661 cgcgggctga cgctatccgg cggccagcag cagcgcgtcg ccctcgcgcg cgccttgctc
      721 accgacccgc gcatcctcat cctggatgat tgcacctcga gcctcgacac gcgcaccgag
      781 caccgcatcc agacgcgcct ggcctcctac atggcggggc gcactacctt catcatcgcc
      841 cagcgcgcct cttccgtggc acgcgccgac cgaatcttcg tcctcgaaaa cggcgccatc
      901 gttgaggaag gcgcgcctga cgatctcctg cggaatccgg agagcctctt cgcccgcctc
      961 taccagctgc aagagcaggc gggaagccgc gccggcgaat ccggtttgcc tgcgcccgcc
     1021 tccgggtaag tctaccaaga atacccctcg gaggtggagc gttgaccgag gcacaccatc
     1081 cttccctcga tacgggcctc atcgagagcc tgaccgacct ggagcgcgcg agagagattg
     1141 ccgccggctg cgtgcggtgc ccgctgaagg agacgcgcac cctggtggtc tttggcgaag
     1201 gcaatccagg ggccagcctg atgcttgtcg gtgaaggccc cggtgaggaa gaggacgcgc
     1261 ggggccggcc cttcgtgggg cgtgccggac agctcctgga tagtgcactc gcggaggcgg
     1321 ggatcccgcg cgacgcggtg tgggtgacga acacggtgcg ctgccggccg acgaagattg
     1381 aggccgggcg ggtagccaac cggccgccgc gtgtcgggga gatccgcgcg tgtgccgtct
     1441 ggatggagac ggagctgcgt ctcgttgccc cgcgcgttgt tgtctgtctc ggcgcggtcg
     1501 ccgccaaggc gctcatcggc aaggagtttc gcctcaccca ggagcgtggc cagctcttcc
     1561 ccggaccggc gggcacggag gccgtggcga cgctccaccc ctcctacatc atccgccagc
     1621 gcgactcgga atcgcgcgcg cgggcgcggg agatgctcgt ggaggacctg cgcctggcga
     1681 tccgccgagc aggcgcgtag cggtccacac gccagcggcc agtcggttga tccccaccgg
     1741 ctgaggccgg cggcgccgcg gccggcgcag gaggaacggc ctgagagcgg cgcgaatcgg
     1801 tgattgcgag cacgccacgc aggtggctgc aggcagagga tgagaggagc gatgatgact
     1861 ggaccccgag cgtgtgcccc gagtgagttg ccacagctgg cgcgcctagc ggatgctgtc
     1921 ttccgctcga aaggcggcag catgcaggcg gagtatcccc tggtgttcgg cgaggagaac
     1981 ctcaactttt gccgcgtgat tgaggaggat ggccgcatcg tctcgcacgt gggcgtctcg
     2041 atccgcgatg cttctctcct cggatgcagc ctgcgggttg cttccatcgg cgcggtgtgc
     2101 accgatgctg cctaccgtgg caggggatac gccagtctcc tcatggagga cgcacgcacg
     2161 ctggccctgg gcgagggggc gtcgctcatg ctcatctccg gtggccgcgg gttgtatcat
     2221 cgccttggct atgtcaacgt gggcagcttt cgctccttca ccgctcgtgc ggagatactg
     2281 ccggtgccag cggagggctt gcgcgtggag ccctgcggcg cagcgcagat tccggggctc
     2341 atcgctttgc accagcgcct ccccgtgcgc ttcgtgcgcc ctgcggatga ttgggagaag
     2401 ctattggccg ccgggatgat catgaaccag cgtggggatt gcctcggcgt gtgggaagaa
     2461 ggccggttgg tggcctatgc cgcggtgcag cgcccgcgag atggcgcgcc ggcgcgcgta
     2521 agggagtatg cgggttcgga gacggcgctt ttcgcggctc ttggcgcagt cgcccgctac
     2581 tacgaggcgt ccggcgttga ggtcgtggcg atgccgggaa cggaggatct cgcccggttg
     2641 ctgcgaaacg ccggcgccga ggagaaggag ctcgccttca cgggcacgct ggggatcctc
     2701 gatgtggatg gcttctttgc ctgcatccat ccctacgtgc gcgagtgttt gggtgctccc
     2761 accgacgcgc tcttcctcga cccccgccag acgaatagca tcagcttcga gtacgcgggc
     2821 gagcggtatg cactcgccaa tcgtggtcag ctcaccgcgc tcgtcttcgg cgggtccacc
     2881 gaggaggcac ggagcatccc cgaagcaccg ccaggcgtcg gggagatcct gaagcgcatc
     2941 ttccccatgc cgctcctctg gtatggctac aactacgtct aatcacgcgc cctgagaacc
     3001 ggcgcgttca gggatcagcc ttcggcgggg aagttgcggc gggagagctg agggagaagg
     3061 tgccggtagg ggagagtagt atcagcgcga gcgcgttgct ggaacgggtc gctgcaacgc
     3121 gctcgcgtgc ttagtctact tgcgccactc gccgtagccg gtgaggtggt agccgatgcc
     3181 gacgtaatcg aggacgcggc agagcgagcg caacgcggcg ccgaagccat ccggcccgga
     3241 gtagccgccg aactgcccgc cgcccttgac gtggggctcc agatccagga agacgcccgg
     3301 cacgccgcgg ctctgcagct ccgcggtcag ctccgggagg cgatccttga agtcgcgcag
     3361 gatccgctcg tggccggagt cgccctggtc ggcgggcacc atgcggttga gctgctcctc
     3421 gttgacatag ccctgccact cctcgccggg cgcgcgctca taatccttga tgtgcatcca
     3481 gccgatatac ggcttcatcg ccgtgtactc gtgataggtc tcttcggtat cgtagccctg
     3541 gcagacgatg tttgccccgt cgaagatcag gtagaggtgg tcgttgttga tccgatcgcg
     3601 aatggcgcgc aggagcgtgc cgttgcgccc gaccaggttg gcttccacct cgaggccgta
     3661 gaaaacgccc tccgccttgc acttttccac gacacggccc acatagtcag cggcctggtc
     3721 gacgtactgc cacggatccg cgtccttggg cgggtagaag gagaagcccc gaatcagctt
     3781 cgtaccgaaa gaatgcgcca gggagatggc gcgcggcact tccttctcca gatactcgtc
     3841 gatcggcatg tagacgttgt gcgtgccgtc ttcctggtcg accagtttga tcttgccgat
     3901 gggcgagccg atggaggaga cctgaacctc gaactggtca tgcagctcgc agaggcgttt
     3961 gatctcggcg tcgctcagct tcatgacgtt cttgacgccc tcgcctacgt ccacgaaccg
     4021 gatggagtag taggacatcc cgagcgcccg aagcatggta aactgctctt cggcgcgctt
     4081 gctcccttcg tctgcaaagc cgctcagtat gacttgtggt gcgctcactc gtgtttcctc
     4141 ccaaggcgat gagaaagggc gagagacgcg ggtcgaagtc ggcgatggcc gtcgccccgt
     4201 cttcgccggc ttgagtatcc ttccacccag atccttcggg cctcgggccc tggagtcctt
     4261 ggcggatctc ctctcaacaa gggctgggtg agccagatgc tcgcggcagc ggcgctcgtg
     4321 gctgcggcgg ggttactccg cgcgctctgc gttcagggcc tcccatcgca gtgccattcg
     4381 gattcggttg ccaatagagg gatgggtata gaggagcgcc tcgatcaggg ccgagggcgc
     4441 cggatcggcg aggttttggg cagcgagctt gcgcatgact ccgatgaagg cgtccggctg
     4501 acgcgtcatg tcgagcgcga agcggtcgca ctggcgctcc cacacgcggg agagggcatt
     4561 ccctgcgggc atcagcacgg tgcttgccag gcctagaaaa agggccagtg acggcagcgt
     4621 ggcgatatcg cccttcccgg tgtagccagg tcg
//
LOCUS       DAOSCV010000265         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11698_length_4640_cov_4.089858, whole genome shotgun sequence.
ACCESSION   DAOSCV010000265 DAOSCV010000000
VERSION     DAOSCV010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11698_length_4640_cov_4.089858"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLY56_07830"
     CDS             <1..1028
                     /locus_tag="PLY56_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21429.1"
                     /translation="KAAAVKAGLPETIVARCVDSVCGSALEAVNMTVEAIAAGVMQVG
                     VAGGMEARSQAPYLLGPKFTRNSGEFQKGKRPRMKRAGAYRWALDGAEPEQLKALEIK
                     DSVSYDGLFWPTERKFMREYALAFAKKQGYTVEQINAYAEQSHRKARLAIESGWFVDE
                     IAPCGDVAVDQVVSDEKLAAMREECADDPAGSYNASVPADGAAAVVVASGAAVKALGL
                     TPMARVVACGRVHCPAPEFLTAPVDAANELSEALRAAGQPGDFPIIEANEAFGIQLPF
                     FEASFPGAAINVHGGAVALGHPLGAAGARLLTTLLYAMKRYDHPRGLVTLCFGGGGAV
                     AIAVERC"
     gene            1038..2240
                     /locus_tag="PLY56_07835"
     CDS             1038..2240
                     /locus_tag="PLY56_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOJ21430.1"
                     /translation="MKLVQFGAGNIGRSFIGQLFSRAGYEVVFADVVPEVIRLLNERR
                     RYRVEIKDDPPGTIWVENVRAVDGRDRDQVVAELTTADIAGTAVGPAALPHLFPVIAA
                     ALRARREQGRGPLDIILCENLRGAAATVREGVAALLPGDFPLDESLGLVETSIGKMVP
                     IMTAAQRAADPLAVHAEAYNTLICDARGFRGPIPNVPGLDPKQNMRAYVDRKSFIHNL
                     GHAITAYVGYVCDPRMVYLWEAVERPDVREVAEGAMWESARALIREYPEEFTEANQRE
                     HIEDLLRRFANRALGDTIFRVGRDLPRKLSPEDRLVGALRLGLRHGLSAPNTLLGIAA
                     ALHFRAVDEEGRLHPADAQFVEEWIPQGPEKILTGVCALNPDVPAEAACIHEVTAAYH
                     RLGSGAGL"
     gene            2367..>2860
                     /locus_tag="PLY56_07840"
     CDS             2367..>2860
                     /locus_tag="PLY56_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041734229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOJ21431.1"
                     /translation="MDIIGQIKARAKSAGLTIALPEGEEERNVIAAGQVMREGIAKII
                     LLGREKEVRATAARVGTDLSDIPIVDPSTDPRFEEFSNRFYEMRKAKGMTPDQARVAM
                     CDPIHFAAMMVKLGQADGYVSGAIHATAEVLRPALTIIRPARGIKTVSAWFLMVLPDP
                     TYGA"
     assembly_gap    2861..2870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2871..3411
                     /locus_tag="PLY56_07845"
     CDS             <2871..3411
                     /locus_tag="PLY56_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOJ21432.1"
                     /translation="VLAYGDSGMVIDPDAEQLAEIAIETAESFHALTGAEPRVAMLSF
                     STKGSAKHPLVDKVVQATELVRKRAPDLLVDGELQADAALVPSVAKTKVKGESPVAGR
                     ANVLIFPNLDCGNIAYKLTERLAHAGAYGPLIQGLARPVNDLSRGCSAEDIVNIVAIT
                     ALQAVSAREHEKNAVGTDG"
     gene            3408..4502
                     /locus_tag="PLY56_07850"
     CDS             3408..4502
                     /locus_tag="PLY56_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ21433.1"
                     /translation="MKKVFIAATHQNEGKTTLSLGLLAALQNRGLDVGFIKPVGQRYT
                     EVNGQKIDEDAVLVAAVMDREEELAAMSPIAVESRFTREYIDAPRPDDLLDRITAAFH
                     QVSQGRDFMVIEGTGHAGVGSVFDLSNARVAQHLGAGVILVTGGGIGRPIDEIMLNRC
                     LFAAHGVPVIGVIINRAIPEKLEMIRDYAGRGLARFGVDLLGVIPLRPRLSNPTVRQV
                     VDGLKGRLLNGGDSLDHKIERCVIGTMSPHTALDHFAPGVLAILPGDREDLLLAAMSS
                     CVMGERQSYCVSGIVLTRGILPHATVMRLVKRTQIPVVLVDDESYSVVSKIHDLTVKM
                     QPNDTETIQLAQRLVEKHVDIDRVLAAATS"
BASE COUNT          773 a         1509 c         1528 g          820 t
ORIGIN      
        1 cgaaggcggc cgcagtgaag gctggtctcc ccgagaccat cgtcgcccgg tgcgtcgatt
       61 ctgtttgcgg ttccgcgctc gaggcggtga atatgaccgt ggaggcgatt gccgccgggg
      121 tgatgcaggt cggcgtagcg ggtggcatgg aggcgcgcag tcaggcgccg taccttctcg
      181 gtcccaagtt cacgcgcaat tcgggcgagt tccagaaggg aaagcgaccg cgcatgaagc
      241 gcgccggcgc ctaccgctgg gccctcgatg gcgccgagcc ggagcagctc aaggcgcttg
      301 agatcaagga ttcggtcagc tacgacggcc tcttctggcc taccgagcgc aagtttatgc
      361 gcgaatatgc ccttgccttt gccaagaagc agggctacac cgtggagcag atcaacgcct
      421 atgccgagca gagccaccgc aaagcgcgcc tggcgatcga gagtggctgg ttcgtcgacg
      481 agatcgcgcc gtgtggcgac gttgccgtcg accaagtggt ctccgacgag aagctggcgg
      541 cgatgcgcga ggagtgcgcc gacgatccgg cgggcagcta caacgcctcc gtgcctgcgg
      601 atggcgccgc ggccgtcgtc gttgcttctg gcgccgccgt gaaggcattg ggactgacgc
      661 ctatggcccg ggtggtggcg tgtgggcgag tgcattgccc ggcaccagag tttctcaccg
      721 cgcccgtgga tgccgccaac gagctctcgg aggcgctgcg cgccgcgggc cagcccggcg
      781 atttcccgat catcgaggcg aacgaggctt ttgggattca gcttcccttc ttcgaggcga
      841 gcttccccgg cgccgcgatc aacgtccacg gcggggctgt cgccttgggt cacccgctcg
      901 gcgccgctgg cgcacgtctg ctcaccacgc tcctctacgc gatgaagcgc tatgatcacc
      961 cgcgcggcct ggtcactctc tgcttcgggg gcggtggcgc cgtcgcgatc gctgtcgaac
     1021 gctgctgagg gaatactatg aagcttgtac agttcggcgc cgggaacatc ggccgctcct
     1081 tcatcggcca actcttctcg cgtgccgggt atgaagtggt ctttgccgat gtcgtgccag
     1141 aggtcatccg cctcctcaat gagcgccggc gctaccgcgt cgagatcaag gacgacccgc
     1201 cgggaacgat ctgggtagag aatgtgcgcg cggtggacgg ccgcgaccgt gaccaggttg
     1261 tcgccgagtt gaccaccgcc gatattgccg gcacggcagt aggacccgct gctctcccgc
     1321 acctctttcc ggtgatcgcc gcagcgctcc gcgcgcgccg ggagcagggc cggggcccgc
     1381 tcgatatcat cctctgcgag aacctgcgcg gggcggctgc cacggtgcgc gagggcgtgg
     1441 ccgccctctt gccgggtgat ttccccctgg acgaaagcct gggcctggtg gagacgagca
     1501 tcggcaagat ggtgccgatc atgaccgcgg cacagcgcgc cgccgatccc ctcgccgtcc
     1561 atgccgaggc ctacaacacg ctcatctgcg acgcgcgcgg cttccgcggc cccattccga
     1621 acgtgccggg gctcgatccc aagcagaaca tgcgtgccta cgtggaccgc aagtcgttca
     1681 tccacaacct gggacacgca atcaccgcct acgtcggcta tgtgtgcgac ccgcgcatgg
     1741 tctatctgtg ggaagcggtg gagcgtccgg atgtgcgcga ggtagccgag ggggcgatgt
     1801 gggagtcggc gcgggcgctt atccgcgagt atcccgagga gttcacggag gcaaaccagc
     1861 gcgagcatat cgaggacctt ctgaggcgct ttgccaaccg tgccctgggc gatacgatct
     1921 tccgcgtggg ccgggacctg ccgcgcaagc tctcccccga ggatcgtctg gtcggagcgt
     1981 tgcgcctcgg cctgcgccac ggcctttccg cgccaaacac gctgcttggc attgctgcgg
     2041 ctctccattt ccgcgcggtc gatgaggagg gccggctcca tcccgccgac gcgcagttcg
     2101 tcgaggagtg gattccacag ggccccgaga agatcttgac cggagtgtgt gccctgaatc
     2161 cggatgtgcc cgccgaagcc gcgtgcatcc acgaggtgac ggccgcctac caccgcctcg
     2221 gatccggcgc cgggctgtag cagcgcgccc cgaaaccatc gtggcttccg gcaggtgaac
     2281 gcgcgggggc atgataaagt agttcagtga tcccgttcgt ccggggccac tcccggcgat
     2341 cgctgtggga tgaggagata agactgatgg atatcattgg gcagatcaag gcgcgcgcga
     2401 aaagcgccgg cctgaccatc gcgcttcccg agggggagga ggagcggaac gtcattgctg
     2461 ccgggcaggt gatgcgcgag ggtattgcaa agatcatcct cctgggccgt gagaaagagg
     2521 tgcgcgcaac cgccgcgcgc gtgggtaccg atctcagcga cattccgatt gtggatccct
     2581 ccaccgaccc tcggtttgag gagttctcta accgcttcta tgagatgcgc aaggccaagg
     2641 ggatgacccc ggaccaggcg cgcgttgcca tgtgcgatcc gatccatttt gccgccatga
     2701 tggtgaagct tggccaggcg gatggctacg tctcgggcgc gatccacgcc accgccgaag
     2761 tgcttcgccc ggcgctcacg atcatccgtc cggcccgcgg gatcaagacg gtttccgcct
     2821 ggttcctgat ggtcctcccg gatcccacct acggcgcgaa nnnnnnnnnn ggtccttgcc
     2881 tacggcgata gcggtatggt gatcgacccg gacgccgagc agctggccga gatcgcgatc
     2941 gaaaccgcgg agtcgttcca cgcgctcacc ggtgccgagc cgcgcgttgc catgctctcc
     3001 ttctccacca aggggagcgc gaagcacccg ctggtcgaca aggtggtgca ggcaaccgag
     3061 ctggtgcgaa agcgcgcccc ggatctgctc gtggatggcg agctccaggc cgatgcggcg
     3121 ctcgttccct ccgtggccaa gacgaaggtc aagggcgaga gccccgtggc cggacgtgcc
     3181 aacgtgctga tcttccccaa cctcgattgc gggaacatcg cctacaagct caccgagcgc
     3241 ctggcgcacg cgggggccta cggaccgctt attcagggcc tggcccggcc ggtcaacgat
     3301 ctctcgcgtg ggtgttccgc ggaggatatc gtcaacatcg tcgcgatcac cgcgcttcag
     3361 gccgtctccg cgcgcgagca tgagaagaac gccgtgggta ccgacggatg aaaaaggtct
     3421 tcatcgccgc cactcaccag aacgagggga agacgacact ctctctcggg ttgctggctg
     3481 ctctgcagaa ccgcgggctc gatgtcggct tcatcaagcc ggtcggccag cgctatacgg
     3541 aggtcaacgg ccagaagatc gacgaggatg ccgtgctcgt cgctgccgtg atggaccgcg
     3601 aggaggagct ggcggcgatg agccccatcg ccgtggagtc gcgtttcacc cgcgagtata
     3661 tcgacgcgcc ccggcctgat gacctgctgg atcggatcac agccgccttc caccaggtgt
     3721 cgcagggccg agacttcatg gtgatcgaag gcaccggcca tgccggcgtt ggctccgtct
     3781 tcgatctctc caacgcgcgc gtagcgcagc acttgggcgc gggggtgatc ctggtgaccg
     3841 ggggtggcat cggccggccg atcgatgaga tcatgctcaa ccgctgtctc ttcgccgcgc
     3901 atggcgtgcc ggtgatcggg gtgatcatca accgcgcgat tcccgaaaag ctcgagatga
     3961 tccgcgacta tgccggccgt ggcctggcgc gcttcggcgt cgatctcctc ggggtgatcc
     4021 ccctccggcc acgcctctct aacccgacgg tgcgtcaggt cgtggatggc ctgaaaggcc
     4081 ggctcctcaa cggcggcgat tccctcgacc acaagatcga gcgctgcgtg atcggcacca
     4141 tgtcgcctca tacggcgctc gatcacttcg ctcccggtgt cctggccatc cttcccgggg
     4201 atcgggagga cctgcttctt gccgccatga gctcttgcgt catgggcgag aggcagtcgt
     4261 attgcgtctc tggcattgtg ctcacgcgcg gtattctccc gcatgccacc gtgatgcgcc
     4321 tggtgaagcg cacccagatt cccgtggtgc tcgtggacga tgagagctac tcggtcgtct
     4381 ccaagatcca cgatctcacc gtcaagatgc agccaaacga taccgaaacg atccagctgg
     4441 cgcagcgtct ggtcgaaaag cacgtcgata tcgatcgcgt gctggctgct gccacgtcat
     4501 agcacgccgg gagtcgggcg tcgtctcgcc tccccggttt ccccaatcat gctccaggcg
     4561 aagaaggacc ccccaactcc cccgcggaat catctttcat tctgaaagga gaagccaatg
     4621 acaccgagag cacgcgtgga
//
LOCUS       DAOSCV010000266         4637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11712_length_4637_cov_3.040375, whole genome shotgun sequence.
ACCESSION   DAOSCV010000266 DAOSCV010000000
VERSION     DAOSCV010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11712_length_4637_cov_3.040375"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2662
                     /locus_tag="PLY56_07855"
     CDS             <1..2662
                     /locus_tag="PLY56_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21434.1"
                     /translation="SLSKVTGLLERAATRGDHWLATPLPDLWASEAVDPRADRFQARH
                     ATAQVRLVSVEASRVILESVGGFAKVDGSAIPLARRLRFTFDADGTVQVDVSVEARGA
                     LLLRWLSLGQNEVDPASCRFLAHEGDAAEGFATARPACHAVGEGDLDLGGRFIPWIHF
                     GNDRGGFEVVFPHSDRVSWGWTDTSPYPTGDPLGRAGEGMVIRVASGRPSWEAFAIRN
                     LRTPVDEGWKWSDTFYLSLLPGAMSDPRANDLRVWWPGPHQYRSGWHPPDEKSIAAWA
                     AAGANLVIGGANWRSGDYSHPERPADLDRFIELCHRYGMRVLPYVTFTDLEFGTPAFA
                     AHAEEWRIEPVAEFNYRSHLMCYGAEGWQEHWEREVSAAWERHAFDGLYIDFWAGRLL
                     CRNERHGCAGPYGRCTAPGLRRMARYAADLVRKRQGLIIANTNILPLGMLNSSFDARL
                     LGEWRDPEEADPLSQRVFYNAHRFGCANLLLTGKLQSIDERALALTEAYQGTPVLSGG
                     RTPDERDLLSRRARLLASFGVGAAHAENAFELPPLPGAPGVRASIYRRHDRDECLVTL
                     SNPGAEDVRVPLTALVPLCGPLAGRMVVCVEATRVFTASELSTGEGPYPSVDVPAGSL
                     RTLWIRPDPGAPCLIYALTGRERPSAEWSQEDHALSFTSEHPSLSTAEVLLAGPEPIG
                     IAGDAAVEFQVGIGCVRAEVPCNVPVVASYPPPRLAQLKMRFTRFDRLPEARLPEGYE
                     VRAYRPGDEAAWAAILNATESLGKWDVDRVVRLLQGQRHAVPEGTFFVTWKGLPVATA
                     TTVIGPGNLLPEVGWVGVVPAHQGHRLAFHVCLAVLRYMRDRGFQETYLLTDDFRLPA
                     IKTYLRLGFEPEIADPSHPARWQRILSEIG"
     gene            complement(2680..3480)
                     /locus_tag="PLY56_07860"
     CDS             complement(2680..3480)
                     /locus_tag="PLY56_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ21435.1"
                     /translation="MKLALSARQFMIPGGFSMSLPEFIAFAREVGYDGVEIRRGHLDE
                     TSPAAAVDEARAALEKHGVCCTFLTAPGISDPESLRRAERVVDIASAIGALYVRVSPN
                     SEQSIPWIRRIADYAAERGVKTGAQLHNGTLLDTTERCEIYLPRIDHPNFGLAFEASH
                     LIIAGQEKHAETEIERLAPYIIAVSIQNHKPMPADAEGPEVITLNGRKYALCLPGDPD
                     GVDFDSLFRGLKRIGFDGYLTVMPAAFPGMESTELARVYHNFLSAYLT"
     gene            3766..>4637
                     /locus_tag="PLY56_07865"
     CDS             3766..>4637
                     /locus_tag="PLY56_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21436.1"
                     /translation="MRFVTIGFAHGHIDAAVQGFRKLPGLELVGIADVNSERLADRQA
                     KLGVPAFSDYREMLDTLKPDFAAVCPANADKAAVIVECAKRGVHVFADKPLVTRMPQL
                     TQVEQAVQASGIEAWAYLELRYSPYGYTIKKRIEAGEIGEVVSVYATGPHKLNLPSRT
                     PEMLDADRNGGCLVDLGCHDIDLARWFTRREPIEVTAYLSKRRFLQLDNFWDTAQAFF
                     RFEGDISVYIEPSWLQPDASRFWGDIRITVVGTLGTLEYRTCDSSLMLVTVESGEQAI
                     VAEPAPVGILQDFVA"
BASE COUNT          721 a         1603 c         1538 g          775 t
ORIGIN      
        1 ctcgctttcc aaagtgacgg ggctcctgga gcgcgccgcc acccggggcg accactggct
       61 cgccaccccg ctcccggacc tgtgggcctc cgaagccgtg gatccgcgcg cggaccgctt
      121 ccaggcgcgc catgccaccg cccaggtccg cctcgtctcc gtcgaggcca gccgggtgat
      181 cctcgagagc gtgggcggct tcgcgaaggt cgacggctcc gccatccctc tcgcgcgccg
      241 gctccgcttc acgttcgacg ccgatggcac cgtgcaagtg gacgtgagcg ttgaggcccg
      301 gggggcgctc ctcctccgct ggctttccct cgggcagaac gaggtggatc ccgcctcgtg
      361 ccgctttctc gctcacgagg gggatgccgc agaaggcttc gccacagcgc ggcccgcatg
      421 ccacgcggtt ggcgagggcg acctggatct cggcgggcgc ttcattccct ggatccattt
      481 cgggaacgac cggggtgggt tcgaggtcgt ctttccccat tccgaccggg tctcgtgggg
      541 ctggaccgac accagtccct atcccacggg cgaccctctg ggccgcgccg gcgagggaat
      601 ggtgatccgc gtcgcgtcgg gccggccctc gtgggaagca ttcgcgatcc gcaacctgcg
      661 cacccccgtg gacgagggat ggaagtggag cgacaccttc tatctctcgc tcctcccggg
      721 cgccatgtcc gatccgcgcg cgaacgacct gcgcgtctgg tggcccggcc cgcaccagta
      781 ccgcagcggt tggcatccgc ccgatgagaa gagcatcgcc gcgtgggccg cggcgggcgc
      841 caacctggtg atcggcggcg ccaactggcg ctccggcgat tactcgcatc cggagcgccc
      901 ggccgatctc gaccggttca ttgagctatg ccaccgctac gggatgcgcg tgctccccta
      961 tgtgacgttc accgacctgg agtttggcac gcccgcgttc gcggcgcacg cggaagagtg
     1021 gcggatcgag ccggtagcgg agttcaacta ccgcagccac ctgatgtgct acggcgccga
     1081 gggttggcag gagcactggg agcgtgaggt aagcgccgcg tgggagcgtc acgccttcga
     1141 cggtctctat atcgacttct gggccgggcg cctcctctgc cgaaacgagc gccacggctg
     1201 cgccgggccc tacgggcggt gcaccgcgcc cggcctgcgg cgaatggccc ggtatgccgc
     1261 cgatctcgtc cggaagcgcc aggggctgat catcgccaac accaacatcc tgcccctggg
     1321 catgctgaac agcagcttcg acgcgcgctt gctcggcgag tggcgcgacc ccgaggaggc
     1381 cgatcccctc tctcagcgtg tcttctacaa cgcccaccgc ttcggatgcg cgaacctgct
     1441 tctgacggga aagctgcaga gcatcgacga gcgcgcgctt gcgcttacgg aggcctacca
     1501 gggaacgccg gttctgagtg gcgggcgcac gccggacgag cgtgacctgc tctcccgccg
     1561 ggcgcggcta ctcgcctcgt ttggagtggg cgcggcacat gccgagaacg cgttcgagct
     1621 gccgccgctg ccgggcgcgc cgggcgtgcg cgccagcatc taccggcgtc acgaccgcga
     1681 cgagtgcctt gtcaccctct ccaatccggg cgccgaggac gttcgcgtgc cactcaccgc
     1741 gctcgtgccg ctctgtgggc cacttgccgg ccgcatggtc gtctgcgtgg aggcgacacg
     1801 cgtcttcacc gcgagtgagc tctccactgg cgaaggcccc tacccctcgg tagacgtgcc
     1861 tgctggcagc ctgcgcacgc tctggatccg cccggatcct ggagctccct gcctgatcta
     1921 cgcgctcacc ggccgcgagc ggccatccgc cgagtggtcg caagaagacc acgctctctc
     1981 attcacgagt gagcacccgt cgctttcgac cgcagaggtg ctcctcgccg gtccggaacc
     2041 gatcgggatt gccggtgacg cggcggttga gttccaggtg gggatcgggt gcgtgcgcgc
     2101 ggaggtgccg tgcaacgtcc ccgtggtggc aagctacccg ccgccgcgcc tggcccagct
     2161 gaagatgcgg ttcacgcgct ttgaccggct gcccgaggcg cgcctgcccg aggggtatga
     2221 agtgcgcgcc taccggcccg gggacgaggc cgcttgggcg gcgatcctca acgccaccga
     2281 aagcctgggc aagtgggacg tggatcgcgt ggtccgcctg ctgcaaggcc agcgtcacgc
     2341 cgtgcccgag ggaaccttct tcgtcacgtg gaaaggcctg cccgtggcaa cggcgaccac
     2401 cgtgatcggc ccgggcaatc tcctgcccga ggtaggctgg gtgggcgtcg tacccgcgca
     2461 ccaggggcac cggctggcct tccatgtctg cctggcggtc cttcgctaca tgcgcgaccg
     2521 tggtttccag gagacttacc tgttgaccga cgatttccgc ctgcctgcca tcaagaccta
     2581 cctgcgcctc ggcttcgagc cggagatcgc ggatccgagc caccccgccc gctggcagcg
     2641 catcctctcc gagatcggct gacgcagtga aaacaggcgt caggtaaggt aagcgctcag
     2701 gaagttgtgg tagacgcgcg cgagctccgt gctctccatg cctgggaagg ccgcgggcat
     2761 cacggtcagg tagccgtcaa agccgatgcg cttcaggccc cggaagagcg agtcgaaatc
     2821 gacgccgtcg gggtcgcccg gaaggcatag cgcgtacttg cgtccattga gcgtgatcac
     2881 ctccgggccc tccgcatccg cgggcatcgg cttgtgattc tggattgaga ctgcgatgat
     2941 gtagggagcc agacgctcga tctcggtctc ggcgtgcttc tcctgcccgg cgatgatcag
     3001 gtgcgacgcc tcgaacgcca gcccaaagtt cggatgatcg attcgaggca ggtagatctc
     3061 gcaacgctcc gtggtatcga gaagcgtgcc gttatggagc tgcgcccccg tcttcacccc
     3121 gcgctccgcg gcatagtcgg caatccggcg gatccagggg atcgactgtt cactgttggg
     3181 gctgacgcgc acatagaggg cgccgatcgc cgaggcaata tccaccacgc gctcggcccg
     3241 gcgaagcgat tccggatcgg agatcccagg cgcggtgagg aaggtgcaac acaccccgtg
     3301 cttctccagc gccgcgcgcg cctcatcgac cgccgcggcg ggggacgtct cgtcgagatg
     3361 gccgcggcgg atctcaacgc cgtcgtagcc cacctcgcgg gcaaaggcga tgaactccgg
     3421 caggctcatc gagaacccgc ctgggatcat gaactggcgt gcggaaagcg cgagcttcat
     3481 tgcgacctcc gtatggcgcg gcgagggtcc tggtctggct gctccacacc agcggtaatc
     3541 cgccacgcgc cctgccatcc tgttcgctct cccgcgccat tctcctgccc cccgccaagc
     3601 ccaggcatga acccatcagg gggagccaac gcgttgggga ggaaggcacc cctttgcacc
     3661 gaacctgttc tggaggatag agcttgcgcc tggcgccgcc gggaacctcg catcgtatcg
     3721 gcgcgccgga cgcgcccggc tcggctctct ctagaggagg ctatcatgcg cttcgttacc
     3781 atcggtttcg cgcacgggca catcgacgcc gccgtgcagg gcttccggaa gctgcccgga
     3841 ttggagttgg tcggcatcgc cgacgtgaat tccgagcgct tggctgaccg gcaggcgaag
     3901 cttggcgtgc ccgccttctc cgattaccgg gagatgctcg acaccctgaa gccagacttt
     3961 gcggcggtgt gcccggcaaa cgccgataag gccgcggtca tcgtggaatg cgcaaaacga
     4021 ggcgtgcacg tctttgccga taagccgctg gtcacgcgca tgccgcaatt gacgcaggta
     4081 gagcaggcag tgcaggcctc ggggatcgag gcatgggcct acttggagct gcgctacagc
     4141 ccctacggct acacgatcaa gaaacgcatc gaagccggag agatcggcga ggtggtcagc
     4201 gtctacgcga ccgggccgca caagctcaac ctcccctcgc gcaccccgga gatgctggac
     4261 gccgaccgca acggcggctg cctggtagat ctcggctgcc atgatataga cctggcccgg
     4321 tggttcaccc ggcgcgagcc aatcgaggtg accgcctatc tcagcaagcg ccgcttcctc
     4381 cagctcgaca acttctggga taccgcgcag gccttcttcc gcttcgaggg cgacatctcc
     4441 gtgtacatcg agccaagctg gctgcagccg gacgcttccc gattctgggg tgacatccgc
     4501 atcacggtgg tcggcacgct tggcacgctc gagtaccgca cctgcgatag ctcgctgatg
     4561 ctggtcaccg tggagagcgg cgaacaggcg atcgtggcag aacccgcgcc cgtgggcatc
     4621 ctgcaggatt tcgtcgc
//
LOCUS       DAOSCV010000267         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11744_length_4625_cov_3.024945, whole genome shotgun sequence.
ACCESSION   DAOSCV010000267 DAOSCV010000000
VERSION     DAOSCV010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_4625_cov_3.024945"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLY56_07870"
     CDS             <1..383
                     /locus_tag="PLY56_07870"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HOJ21437.1"
                     /translation="REALTQLYLLVLEHLLRGRELNKVLFSVVAQLPAAHRQLKEDAR
                     EGSRFLSDFLRVRIARGELRPFDTQAAAHFFWGAGYSFFMSHRDHPEEVMRPLLRRFA
                     VEATDIILRGILPEEAAGGSEPEA"
     gene            661..1938
                     /locus_tag="PLY56_07875"
     CDS             661..1938
                     /locus_tag="PLY56_07875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ21438.1"
                     /translation="MAEKLALDGGDRVVPEGMVKAWPPMTQQDKDAVLAVFDSGHLHG
                     SSAPRCLELQERWAEYCGAKYCVVTNSGTAACHMAVATCGVEPGDEVIVPAFTFWSTA
                     AAVLHHNAIPVFVDIDPKTYTIDPKLIEAKITKRTKAIFPVDIHGMPCDMDPIFDIAR
                     KHNLKVISDCCQAHGAKYKGRKVGSIADATAFSCNRSKNLSGGEGGLYTTNNEAWAKH
                     ATMMREFGEVVVAGQPREYNAFGLGWMYRPHEFINAFILSQLTRLDQHNAVRKEFAAY
                     LTEQLAQIPGVKGPYTPDYADPVYFSYVVEFRPQELGLDVPVKEVKNAVIKALRAGGV
                     GLGQWQTMPVPGQSVFQEKKGYGKGCPWTCQFGRDVEYRAEDYPRTIEFIESHSYLSG
                     VYPPNTMELMERYVRGFRKVIDNIDRVMELARE"
     gene            2139..>4625
                     /locus_tag="PLY56_07880"
     CDS             2139..>4625
                     /locus_tag="PLY56_07880"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ21439.1"
                     /translation="MWLLLTFLLSCLATTASAAPGFCPPASPQSGGMRDIMLIYEGGK
                     AWKPEEFRPYVAYVDERGEPKDWFYDAFLFLMYGGAPSGATYIDGATNRADWEHFLNV
                     TFARDRRLDALDQEIEAVGRRLGQGGRVCPVILMIPYPSSRSNAFGDVDGDGVSEDLS
                     RPEDRVKAVRWFLDEALARWKALSPKHLKLWGFYWMNEGIARSDEAIVKATADLVHAR
                     GYGLHWIPWFRAPGLEKWRELGIDFAIVQPNYAFMQPERGFALADQNRLTLNANDAWR
                     LGLGVEMETPFALPGDPGARWLFRQYLNHGDDALDGYQHGAVRAYYQGSDVIARLAAS
                     THPGERQLYDDLYRFHKGTYTRRPTSLAEGARCLLQVAGGTPTPEPRLVDGTWYTRAG
                     REDALIPVKGSSATITLELPAPTLVDDVRLHVAAKELAGLPSAVRVYTADAGSDVFVL
                     RGETIAVPAERSGEWLAGFASVHFFPAPAQRVRIEVAGATDSLFLGEVVMPPAAHPLW
                     GVIARAVGESVEGSAAWLTEGLQDGTPLRWSKGPARLLFDCQEMVGAQVRVRARKAPG
                     RPAPSLRVRTTPEGGWEQGKVVWQDAGCALLAARLPLAPVQHLALEVDGAGEIALEEV
                     QVLPARSWAQHCPYTLSPSFEAKYPDSGGELTDGKLCEQGFGDGRTVGWFSKNPSVVL
                     DLGELREIEGVGAHVEGGGYAAVNFPSRLVVLLSADGETWRPVGSTETVAEKTAERMV
                     GAEIDALGWMRVGFPRQRARFVRFDFTGRVWVMLSELTVFSGGANIARGCAYHLSPTP
                     TSETRYADGDAYLTDGICGSASGGWRTKVGW"
BASE COUNT          762 a         1462 c         1592 g          809 t
ORIGIN      
        1 ttcgtgaggc gctgacgcag ctttatctcc tcgtcctgga gcacctgctt cggggccggg
       61 aactgaataa ggtgctcttc tcggtggttg cccaacttcc ggcagcgcat cgccagctca
      121 aggaagacgc gcgcgagggg agccggttcc tttccgactt tctgcgcgta cgaattgccc
      181 gcggcgagct ccgtccgttc gatacgcaag ccgcggcaca cttcttctgg ggcgcgggct
      241 actctttctt catgtcccac cgggatcacc ccgaggaagt gatgcgcccg ctgcttcgcc
      301 ggttcgcggt cgaggcgacg gatatcatct tgcgcggcat cctcccggag gaagccgcgg
      361 gggggagcga gccggaggct tagccgggcc cgacagccgg ctaccgcggt tctggctgcc
      421 tcatcgctcc ctgcctcctc ctgacctctg ggtagaaaca gcacccctct tgccacaggc
      481 agggaaagag gcagccggct cgaataggga gaagcaagtg ccccagccgg ggcggcgcaa
      541 gctattggct cgtgccccgg cttcgcggtc ctatccggac gcagcgtgct gattgcacgt
      601 gcgccaaccg cgggcggtgt ggccattggc gcaccatcgg ggatcttggg aggatgagaa
      661 atggccgaga agctggcatt ggatggtggc gatcgcgtcg ttccggaggg catggtcaaa
      721 gcatggcctc cgatgactca gcaagacaag gatgcggtgc tggcggtctt cgatagcggg
      781 cacctgcatg gttccagcgc tccgcgctgc ctggagttgc aggagcgatg ggcggagtac
      841 tgcggcgcga agtactgcgt cgtgacgaac agcggcaccg cagcttgcca catggccgtg
      901 gcaacgtgtg gcgtggagcc gggcgatgag gtgatcgtgc ccgcgttcac cttctggtcc
      961 accgcggcgg ctgtgctgca ccacaacgcg atccccgtct ttgtcgacat cgacccgaag
     1021 acgtatacga ttgacccgaa gctgatcgag gcgaagatca ccaagcggac gaaggcgatc
     1081 ttcccggtcg atatccacgg catgccgtgc gatatggacc cgatcttcga catcgcccgg
     1141 aagcacaacc tgaaggtgat ctccgactgc tgccaggcgc acggtgccaa gtacaagggc
     1201 cgcaaggtgg gctccatcgc ggatgccacc gcgttctcgt gcaaccggtc gaagaacctc
     1261 tccgggggtg agggtggcct ctacaccacc aacaacgaag cctgggcgaa gcacgccacg
     1321 atgatgcgcg agtttggcga ggtggtcgtc gccgggcagc cgcgcgagta caacgccttt
     1381 ggcctggggt ggatgtatcg cccgcacgag ttcatcaacg cctttatcct cagccagctc
     1441 acccggttgg accagcacaa cgccgttcgc aaggagttcg ctgcctacct cactgagcag
     1501 ctggcgcaga ttccgggcgt gaagggcccc tacaccccgg attatgccga cccggtttac
     1561 ttcagctatg tcgtcgagtt ccgcccgcag gagctggggc ttgatgttcc ggtgaaagag
     1621 gtcaagaacg ccgtcatcaa ggcgctccgc gccgggggcg tgggcctggg ccagtggcag
     1681 acgatgcccg tgcccggaca gtcggtcttc caggagaaga agggctacgg caagggctgc
     1741 ccgtggacct gccagttcgg gcgggatgtg gagtaccggg cggaggatta tccgcggacg
     1801 atcgaattca tcgaatccca ctcgtacctg agcggcgtct atccgccgaa cacgatggag
     1861 ctgatggagc gctacgtgcg cggcttccgc aaggtgatcg acaacatcga ccgcgtgatg
     1921 gagctcgcgc gcgagtagcg ccgcgccttc ctgtcccttc cctggcgccc tgccttgtgc
     1981 tagcgccagg gaagggcgtg aacgccctgg gttggagaaa tggtccacag ccagggctgc
     2041 gcgcatcgtt ccgccttccc cagggggagt ggggcttggt agacggggaa ggatgccagg
     2101 gggatgcccc acgccggacc ccggaagaga ggtgacccat gtggctcctg ctcaccttcc
     2161 tgctttcctg cctcgcgacc accgcatccg ccgcgcccgg cttctgccct ccggcctctc
     2221 cccaaagcgg cggcatgcgc gatatcatgc tgatctacga ggggggcaag gcatggaagc
     2281 ccgaggagtt ccgcccctac gttgcctatg tcgacgagcg gggggagccc aaggattggt
     2341 tttacgacgc cttcctcttc ctcatgtatg gcggcgcgcc ctctggagcg acctacatcg
     2401 atggggccac caaccgcgcc gactgggagc atttcctcaa cgtcacgttc gcccgcgata
     2461 gacgcctgga tgccctcgac caggagatcg aggctgtggg ccggcgcctg ggccagggcg
     2521 gccgcgtctg cccggtcatc ctgatgatcc cctatccctc ttcccgttcg aacgcgtttg
     2581 gcgatgtgga cggcgatggc gtctcggagg acctctctcg gcccgaggat cgggtgaagg
     2641 cagtgcgctg gttcctcgac gaggcgctgg cgcgctggaa ggcgctctcc ccgaagcacc
     2701 tgaagctgtg gggcttctac tggatgaacg aggggatcgc gcggagcgac gaggcaattg
     2761 tcaaagcgac cgccgacctg gtgcacgcgc gtggctatgg cctccactgg atcccgtggt
     2821 ttcgcgcccc cgggttggag aagtggcgcg agttgggaat cgatttcgcc atcgtccagc
     2881 ccaactatgc cttcatgcag cccgagcgcg gctttgccct ggccgatcaa aaccgcctga
     2941 cgctcaacgc caacgatgcc tggcggctcg ggcttggcgt ggagatggag acaccctttg
     3001 ccctccccgg cgatccgggc gcacgttggc tcttccgaca gtatctgaac cacggagacg
     3061 acgcgctgga cggctaccag cacggggccg tgcgcgccta ttaccaggga agcgatgtga
     3121 ttgctcgact ggccgcgagc actcatcctg gcgaacgcca gctttacgat gacctctacc
     3181 ggttccataa aggcacctac acgcgccggc cgacttccct ggcggagggc gcgcgctgcc
     3241 tgctccaggt agccggcggc acgccgacgc cggagccgag gctcgtggat ggcacttggt
     3301 acactcgggc tggccgcgag gacgcgctga ttccggtgaa aggctcttcc gccacgatca
     3361 cgctcgagct gcccgcgccg accctcgtgg atgacgtccg cctgcatgtc gcggcaaagg
     3421 agctcgccgg gctgccatct gcggtgaggg tctacaccgc cgatgcgggg tcagatgtct
     3481 tcgtattgcg cggcgagacg atagcggtcc ccgcagagcg gagtggggag tggctggccg
     3541 ggttcgcttc ggtgcacttt ttcccggcgc cagcgcagcg cgtgcgtatc gaggttgcgg
     3601 gtgccacgga ctcactcttc ctcggcgagg tggtgatgcc cccggcggcg cacccgcttt
     3661 ggggagtcat cgcccgcgca gtgggagaat cggttgaagg cagcgcggcg tggttgaccg
     3721 aaggcctgca agacggcaca ccgcttcgct ggtcgaaggg gccggcgcga ctcctcttcg
     3781 actgccagga gatggtcggg gcccaggtac gcgtgcgagc gcgcaaggcg cccggccgtc
     3841 cggcgccctc cctgcgcgtt cgcaccacgc ccgagggagg ctgggaacag ggcaaagtgg
     3901 tgtggcagga tgcagggtgc gctctcctgg ctgctaggct gccactggcg cccgtccagc
     3961 acctcgcgct cgaagtggat ggggcagggg agatcgcgct cgaggaggtg caggtcttac
     4021 cggcgcgcag ttgggcgcaa cactgtccgt acaccctctc gccttccttc gaggcgaaat
     4081 acccggacag cggcggcgag ctgaccgatg gcaagctgtg cgagcagggc ttcggcgatg
     4141 gccggacggt tggctggttt agcaagaacc cgagcgtggt gctcgacctg ggcgagctaa
     4201 gggagataga aggggttggg gcccacgtgg aaggcggcgg ctacgcagcc gtcaactttc
     4261 cgtcacggct cgtggtcctg ctttccgcgg atggggagac gtggcgccct gttgggtcca
     4321 cggagaccgt cgcagaaaag acagccgagc gcatggttgg cgccgagatc gacgcgctgg
     4381 ggtggatgcg cgtgggcttc ccgcggcagc gggcgcgctt cgtgcgcttc gacttcacgg
     4441 ggcgcgtctg ggtgatgctg agcgagctga ccgtcttctc cggcggggcg aacatcgcca
     4501 ggggctgcgc ctaccacctc tcgcccacgc ccacttccga gacgagatat gccgacgggg
     4561 acgcctacct caccgatggg atctgcggtt cggcgagcgg agggtggcgc acgaaagtcg
     4621 gctgg
//
LOCUS       DAOSCV010000268         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11763_length_4622_cov_3.806875, whole genome shotgun sequence.
ACCESSION   DAOSCV010000268 DAOSCV010000000
VERSION     DAOSCV010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_4622_cov_3.806875"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLY56_07885"
     CDS             <1..1129
                     /locus_tag="PLY56_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21440.1"
                     /translation="RRWRDVNAMRRPDRAPVWCRPVGAWAELLPSEALVCQDPWMRMV
                     ECELRMTLIKHDIGDDTPVEGTFAVPAILERDPLNTWGVEMNRIPPSRPGGAWVYDPP
                     LKRLEDIERLRLPSVRLSKEQTEAALARAHDLLGDLLPVRLTCEPPLNATICTVAAAL
                     RGLTQLMIDMIESPDWVHRLMAHLRDGVLQTMDQVEAMGLLSPNNVGGMFCSDPIGPD
                     PDGGPLTYANLWGCANSQEFDPVSPAMWEEFLLQYQKPILERFGLTAYGCCENLTEKI
                     DGVLSIPNLRLFVCSAWTDLGRVIERVAGRYVIMWRQKASAVVFTDDPEELRRDLEEG
                     MRQLQGCHYQVVLRELQTLAGHPDRLHVWSRLAIETAEKYA"
     gene            1262..2623
                     /locus_tag="PLY56_07890"
     CDS             1262..2623
                     /locus_tag="PLY56_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOJ21441.1"
                     /translation="MPKVTLIGAGSVVFAKNLLSDCLSYPELSEGTYVLYDIDQERLR
                     TAEKMAHQVAKAWNAKPTIVATTNPVEALEDADYAINMIQVGGYEPCTVTDFEIPKKY
                     GLRQTIGDTLGIGGIFRALRTIPVMLEYADIMEEVCPDVTFLNYVNPMAMNTWAMLEA
                     THINTIGLCHSVQSSYRHLCHAIGVNPDEVNYTCAGINHMAFYLTLEHNGEDLYPRIF
                     EKVASREYYPDWDRVRLDMMLRIGYYITESSEHFSEYTPWHIKRDRPDLIEQYNIPLD
                     EYPRRCINQNASWAKMAADLEAGKGFESLRRTSEYGSRIIHAMETGEPTVIHGNVLNY
                     GLIDNLPEGCCVEVPCLIDKNGIQPMRVGELPPHLAAMNRTNINVQELTVRAALEECK
                     TYVYHAAMLEPHTGAELSLDQIWALVDEMFEAHGDWIPLFKDDCECGYECDGDCDHDC
                     HHG"
     gene            2812..3438
                     /locus_tag="PLY56_07895"
     CDS             2812..3438
                     /locus_tag="PLY56_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ21442.1"
                     /translation="MHYLTIAEAAEALHLSVPTIKRYIYEGKLRSTKLPGGQHRIPQS
                     EIDRILSPEAAPGARMEEAAPPSLAERIAVLERWVTELESEVERLTAALEVVSRYCAL
                     HVGDAPHADEKAARSPSHHRLLVLGPGCRRCDTLYDLVQRALLAGGYRNVSVERVKDL
                     DGIAGFGPVLTPALVMDGTVIVAGRIPSGHDLQGILERHLGPPNPPEA"
     gene            complement(3812..>4622)
                     /locus_tag="PLY56_07900"
     CDS             complement(3812..>4622)
                     /locus_tag="PLY56_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21443.1"
                     /translation="VGAGLGAGLDTVKSLGVHTVQVHAPHEPDRAPDRVAQFRQQFAD
                     AGIQVTVVFAGFPGESYADIPTVQKTIGLVPPGPRAERLKETKAISDFAHALGCDALG
                     MHIGFVPEDASDPNYAGVVKAAQEVCDYCKQNGQRFHLETGQEKADVLLRFFNDVGRD
                     NLAINFDPANLILYGTDEPIPALRSVGRYVKSVHCKDAKRKPDVGGGWAGVEVALGQG
                     DVNMELFLRTLKEIGYFGPLTIEREISGEQQKKDIAEAIGLLQRLRTEILG"
BASE COUNT          807 a         1480 c         1553 g          782 t
ORIGIN      
        1 ccggcgctgg cgggacgtga acgcgatgcg ccggccggac cgcgcgccgg tgtggtgccg
       61 tccggtgggt gcctgggcgg aactccttcc gtccgaggcg ctcgtctgcc aggatccctg
      121 gatgcgcatg gtcgaatgcg agttgcgcat gacgctgatc aagcacgaca tcggcgatga
      181 caccccggtg gagggcacct ttgccgtgcc ggcgatcctg gagcgcgatc ccctcaacac
      241 ctggggcgtg gagatgaacc gcattcctcc atcgcgtccg gggggcgcct gggtctacga
      301 tccgccgctg aagcgcctgg aggatatcga gcgcctgcgc ctgccatccg tgcgcctgag
      361 caaagagcag accgaggccg cgcttgcccg cgcccatgac ctcctcggcg atctcctgcc
      421 ggtccggctt acctgcgagc cgcctctgaa cgcgacgatc tgcacggtgg ccgcagccct
      481 gcgtggcctg acccagttga tgatcgacat gatcgagagc ccggattggg tccaccgcct
      541 catggcgcat ctgcgcgatg gcgtccttca gacgatggat caggtggagg cgatggggct
      601 gctctcgccc aacaacgtgg gcgggatgtt ctgcagcgac ccgatcggtc ccgacccaga
      661 cggcgggccc ctcacctacg cgaacctctg ggggtgcgcc aacagccagg agttcgaccc
      721 cgtttccccg gcgatgtggg aggaattcct cctccagtac cagaagccga tcctggagcg
      781 gtttggcctg acggcctacg gctgttgcga gaacctgacg gagaagatcg acggggtgct
      841 ctcgattccg aacctgcgcc tcttcgtctg tagcgcgtgg accgatctcg gccgggtgat
      901 cgagcgcgtc gccgggcggt atgttatcat gtggcggcag aaggcgagcg cggtggtctt
      961 cacggacgat ccggaggagc tgcggcgcga tttggaagag ggaatgcgcc agttgcaggg
     1021 atgccactat caggtggtcc tccgcgagtt gcagacgctc gccggccatc cggaccgcct
     1081 ccacgtctgg agccggcttg ccatcgagac ggctgagaag tatgcttgag tgtgcgggtg
     1141 aagtgtgtcc ccggagggcg tgaaggcgtg atgggagagg gagccggcgc gagcgggctt
     1201 gctgttcttt acggtttcct ggccttcagg gggaagcgag agagagtaga aaggagtgta
     1261 gatgccaaag gtcaccttga tcggcgcagg gagcgtcgtc tttgcaaaga acctgttgtc
     1321 cgactgcttg tcttatccgg agctgagcga gggcacgtat gtgctctacg acatcgacca
     1381 ggagcgcctg cgcacggcgg agaagatggc gcaccaggtc gccaaggcgt ggaacgcgaa
     1441 gccgacaatc gtcgctacca ccaacccggt ggaagcgctg gaggacgccg actacgcgat
     1501 caacatgatc caggtgggtg gctacgagcc gtgcaccgtc accgacttcg agatcccgaa
     1561 gaagtacggg ctgcgccaga cgattggcga tacgcttggg atcgggggca tcttccgggc
     1621 gttgcgcacc atccccgtga tgctcgaata cgccgatatc atggaggagg tctgccccga
     1681 cgtcaccttc ctcaactacg tcaacccgat ggcgatgaac acgtgggcga tgctcgaggc
     1741 gacccacatc aacaccatcg gcctttgcca cagcgtgcag agcagctacc gccacctttg
     1801 ccatgccatt ggcgtcaacc cggatgaggt gaactacacc tgcgccggca tcaaccacat
     1861 ggcgttctac ctgacgctgg agcataacgg cgaggatctc tacccgcgca tcttcgagaa
     1921 ggtggccagc cgcgaatact atccggactg ggaccgcgtc cgcctcgata tgatgctgcg
     1981 catcggctat tacatcaccg agtcgagcga gcacttcagc gagtacaccc cgtggcacat
     2041 caagcgcgac cgtccggatc tgatcgagca gtacaacata ccgctggatg agtacccgcg
     2101 ccggtgcatc aaccagaacg cgagctgggc gaagatggcg gcggacctgg aagccggcaa
     2161 ggggttcgaa tcgctgcgcc gcacctcgga gtatggctcg cgaatcatcc acgccatgga
     2221 gacgggcgag ccgacggtga tccacggcaa cgtgctcaac tacggcctga tcgacaacct
     2281 tcccgagggg tgctgcgtgg aggtgccgtg cctgatcgac aagaatggca tccagcccat
     2341 gagggtgggc gagctgcctc cgcacctggc ggcaatgaac cgcacgaaca tcaacgtgca
     2401 agagctgacc gtgcgcgccg ccctggagga gtgcaagacg tatgtctacc acgccgccat
     2461 gctggagccc cacaccggcg ccgagctgag cctcgaccag atctgggcgc tcgtggatga
     2521 gatgttcgaa gcccacggcg actggattcc cctcttcaag gacgactgcg agtgtggcta
     2581 cgagtgcgac ggcgactgcg accacgactg ccaccacggg taagctccac cgtttggggg
     2641 ttcagggcgg gtgccctgaa ccccaccccc ttccccatcc atagcatccc aggtgcccca
     2701 ccattttccg ccggtgtttc ctcactttgg cctcccaggg caggcaatcc gccgcagtgc
     2761 atggaaccaa gagagcggga gtgcgcgctg cgcactgacc ggaggagcgg agtgcattac
     2821 ctcacgattg ctgaagcagc ggaagcgctg cacctgagtg ttcccaccat caagcgctac
     2881 atctacgaag ggaagctgag atcgacgaag ctgccgggcg ggcagcatcg gatcccgcag
     2941 tcggagatcg accgcatcct ctcgccagag gccgcgcctg gcgcccgcat ggaagaggcc
     3001 gccccccctt ccctcgcgga acgcatcgcg gtgctggaac ggtgggtgac cgagctggag
     3061 tcggaggtcg agcggctcac ggccgcgctg gaagtggtct cacgctactg cgccctccac
     3121 gtgggggacg cgcctcacgc cgatgagaag gctgcgcgct cgccaagcca ccaccgcctg
     3181 ctggtgttag ggcccggctg tcggcggtgt gatacgctct acgacctggt tcagcgcgcc
     3241 ctgctggcgg ggggctaccg gaatgtgagc gtggaacggg tgaaggatct ggacggcatc
     3301 gccggcttcg gcccggtgct taccccggcg ctggtgatgg atggcacggt catcgtggcc
     3361 ggccggatcc cgagcgggca cgacctgcag gggatcctcg aacgccacct aggccctccc
     3421 aacccaccgg aggcatagcg gagaggggcg ccggtgtcgg ttgcccgcgc gtgcaggttg
     3481 gcttagcccg gcagggcttc gctcacaagc agcgcggtga tctatcgccg cgcgatacac
     3541 gaggggccgg ggcaacacac gcgcgcggag ctggaagctc cgcgctgctt ctcggcaaag
     3601 ccccttgcgg ggctccccct ccctgttgcc cgctaccgag ccgcccgcga gccgtaaggg
     3661 cgaatgttca ctgtttgctc tgcttccgta tgggccgggt gggcgctcag ggttgcgccg
     3721 gccatgcggg ggcgcggacg agaggcgccg gggcctccag ggtggactgg gggaggcccc
     3781 gggcgtttgc ctattgctcc agaaggcgag atcagccgag gatctcggta cgaagccgct
     3841 ggagcaggcc gatggcctcg gcgatatcct tcttctgctg ctcgccggag atctcgcgct
     3901 cgatggtgag cgggccgaag taaccgatct ccttgagcgt gcgcaggaag agctccatgt
     3961 tgacgtcgcc ctggcccaaa gctacttcca cgccggccca gccgccgccc acgtccggct
     4021 tccgcttcgc gtccttgcag tggacgctct tgacgtagcg gcccacggag cgcagcgccg
     4081 ggatcggctc gtccgttccg tagagaatca ggttggccgg gtcgaagttg attgccaggt
     4141 tgtcgcggcc gacgtcgttg aagaagcgga ggaggacatc cgccttctcc tggccggtct
     4201 ccaggtggaa gcgctgcccg ttctgcttgc agtagtcaca gacctcctgg gccgccttca
     4261 caacccccgc gtagttgggg tcggaagcat cttccggcac gaagccgatg tgcatcccga
     4321 gggcatcgca acccagggcg tgcgcgaagt ccgagatcgc cttcgtctcc ttcaggcgct
     4381 cggcgcgcgg tcccggcggc accaggccga tggtcttctg gacggtggga atgtcggcgt
     4441 agctctcgcc ggggaagccg gcgaaaacca cggtcacttg gatccccgcg tctgcgaact
     4501 gctggcggaa ctgcgctacg cgatccgggg cgcggtccgg ctcatggggg gcatgaacct
     4561 gcacggtatg cacgcccagc gacttgacag tatctagccc ggctcccagt ccggcgccga
     4621 cc
//
LOCUS       DAOSCV010000269         4622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11764_length_4622_cov_2.604773, whole genome shotgun sequence.
ACCESSION   DAOSCV010000269 DAOSCV010000000
VERSION     DAOSCV010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11764_length_4622_cov_2.604773"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLY56_07905"
     CDS             <1..218
                     /locus_tag="PLY56_07905"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOJ21444.1"
                     /translation="LSLYHDQGHIAAKTRDFYGTVTATLGLPVLRTSVDHGTAFDIAW
                     QGKASAVSMEAAILAAVDLLSQRAGSK"
     gene            481..3453
                     /locus_tag="PLY56_07910"
     CDS             481..3453
                     /locus_tag="PLY56_07910"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ21445.1"
                     /translation="MKLWKQPIALLAGALMLACLMPGAAAPERGEAPRLTLAGPAAEA
                     PVIDGRLDDACWHQAAQTTGFVLNDSREAATEQTTVYVTYDSAHLYVAFECLESQMDE
                     VRATVTERDGPIFKDDVVELFLDPNQDRFTYYQFAVNVRGTRFDLRGDAAGTAPHWDA
                     PWNAATTRGSDRWWAEFAIPFSSLELHVGKTGDTWGINFAREQKPKGELSVWSYTGGA
                     FGRPDRFGQLSGLAVDFSRYAYSIEVADSGERLIGNNVLKARVSSPFGGKALVDLALY
                     LPDGQPQVTSVRTQLNPQTPATVTLPYSLPREGFYRPIVRVRDPNTRQVLRAVVVPIT
                     VPPILELALYPNHYRQEVMLRPRLNVRQEDLSAFRLTARLLKNGTLVPPVREKPFFVA
                     KEPVIPFDLSKSGPGEYEIHVTLTPKRSGGSIVRDVLRFEHMPLSSKSVPPVSIDADN
                     TLLVRGKRFFPIGIYRYGNFTEHSLRELSESGFNLVQAPYAAPLEELREVVDRIHAHK
                     MMTWIPFGPAIELPENDEAKRQKLGALVKAMADHPALLCWESVDEPAWTGSNAADLYR
                     GYRVVRRLDPDHPVWMNHAPRNTVDELALFNHGADVAGADIYPVPEPQTQSDLPNKTI
                     AVVGDETSKNVLAVLNRKPIFMVLQGFAWADLSRRRGGTEKPIYPSFQQSRFMAYHAI
                     VRGARGLLFWGVNYTPKPSPFWNSLRQLAYELSQMHDVLAGPDTGEVAKVHAGGKAIE
                     TLIRRHCETDQRFIIAVNASPEPVDARIEIPRAMPKVSWRVLFEDRRITGNPIVDHFE
                     PWDVHIYTGSLRFPQEKRLELPPVTQVQRPRPEDLLERGNLLVNPGFEFTEDGEESPI
                     GWSLRPAFYGGVSREVKRSGRYSVFLKGRDATSKPLWLQTGIATETGRRYRFTAQVRT
                     EPAGADCRIYAEWTDAAGKYLGGSVPAQWSAGTAQWQPLTLDFMVNHPDAKKLYVVLQ
                     VRGQAQAWFGDVKLEEMR"
     gene            complement(3680..>4622)
                     /gene="ligA"
                     /locus_tag="PLY56_07915"
     CDS             complement(3680..>4622)
                     /gene="ligA"
                     /locus_tag="PLY56_07915"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ21446.1"
                     /translation="HNEDEIRRKDVRVGDTVVIQRAGDVIPEVVSVVTSKRTGAEKPF
                     VFPRECPVCGGPVERPEGEAVARCTGVACPAQAKRRIEHFVSRTAMNVEGIGPAQVEQ
                     LVDRGLVKDPADLYALTLEQLLPLERMGKTLAQKILRNIEASRQVPLARLIFALGIRH
                     VGEHVAELLATHFHTLDTLEAASEEEIREVPGIGPEIAASVARFFADPQTRQLLERLR
                     AAGVQPQAPEAVPAAPADNPIAGKTFVFTGTLAQMTRPDAEALVKSLGGRATGSVSAK
                     TDYVVAGEGAGSKLEKAQVLGITVLTEEEFRALAGLE"
BASE COUNT          758 a         1585 c         1501 g          778 t
ORIGIN      
        1 tcctctccct ctaccatgac cagggccaca tcgccgccaa gacgcgcgat ttctatggca
       61 cggtcaccgc gacgctgggg ctgccggtgt tgcgcacctc ggtagatcac ggcacggcgt
      121 tcgacatcgc ctggcagggc aaggcgagcg cggtcagcat ggaggctgcc atcctcgccg
      181 cggtggatct cctctcgcag cgcgccggat ccaaatagaa cgctgcccgg tatcctgtat
      241 gtccctcgct ttgcacgcat actttctggc gcgccgcccc gcgcaccggc aggcactcct
      301 gcggcccgcg gcgaatagag actctggagg gggtgtcacc gcctccggcg gctctccgct
      361 ctgacggcgg gagatagccg ctccgggccc gtgttgcgca tcgatccgcg ccctttcgcg
      421 cctttccggg tcccaggccg gttcggatgg ccgatccgac cgttccgatg ggagagaacc
      481 gtgaagctat ggaagcaacc aatcgccctc ctcgccggcg cgctgatgct cgcgtgcctg
      541 atgcccggcg ctgccgcgcc tgaacgggga gaagcccccc gcctcacgct ggccggaccg
      601 gcggcggagg cgcccgtgat cgatggccgc ctggacgacg cgtgctggca tcaggcggcc
      661 cagacgaccg gttttgtcct gaacgacagc cgggaagcgg ccacggagca gacgaccgtt
      721 tacgtgacct acgattcggc gcatctctac gtcgccttcg aatgcctcga gtcgcagatg
      781 gacgaagtgc gcgccacagt gacagagcgc gacggcccca tcttcaagga cgacgtagtc
      841 gaactctttc tcgatccaaa ccaggatcgc ttcacttact accagtttgc cgtcaacgtg
      901 cggggcacgc ggttcgacct gcgaggcgac gcggccggca ctgctccgca ttgggatgcc
      961 ccttggaacg ccgcgaccac ccggggcagc gaccgctggt gggccgagtt cgccatcccc
     1021 ttctccagcc tcgagctcca tgtggggaag acgggcgata catggggtat caacttcgcg
     1081 cgcgagcaga agccgaaagg cgagctcagc gtttggtcct acaccggcgg cgctttcggg
     1141 cgccccgacc gtttcgggca gctctccggg ctggccgtgg acttctcgcg gtatgcctac
     1201 tccatcgagg tggccgatag cggcgagcgc ctcatcggca acaatgtgct gaaggcacgc
     1261 gtgagcagcc ccttcggagg caaggcgctg gtggatctgg cgctctatct gccggatggc
     1321 cagccccagg tgacgagcgt tcgcacccaa ttgaatccgc agacgccggc cacggtcacc
     1381 cttccctatt cgctgccccg cgaaggcttc taccggccca ttgtgcgcgt gcgcgaccca
     1441 aacacccgcc aggtgctgcg tgccgttgtg gtcccgatca ccgtgccgcc cattctggag
     1501 ttggcgctct atccgaacca ctaccgccag gaggtgatgc tccggccccg gctcaacgtc
     1561 cggcaagagg atctcagcgc cttccggctc actgcgcgcc tgctcaagaa tggcacgctg
     1621 gtgccgcccg tgcgcgagaa gccgtttttc gtcgcgaaag agccggttat cccgttcgat
     1681 ctatcgaaat ccggtccggg ggaatacgag attcacgtca cgctcacgcc caagcgctcc
     1741 gggggaagca tcgttcgcga cgtcctccgc ttcgagcata tgcccctctc cagcaagagc
     1801 gtcccgcccg tgagcatcga tgcggataac accctcctgg tcagggggaa gcgcttcttc
     1861 ccgatcggca tctatcgcta tgggaacttc acggagcata gcctgcgcga gctgagcgag
     1921 agcgggttca acctggtgca ggccccctat gccgcccctc tggaggagct gcgcgaggtg
     1981 gtggaccgga ttcacgccca caagatgatg acgtggatcc cgtttggtcc cgctatcgag
     2041 cttccggaaa acgatgaggc gaagcggcaa aagctggggg ctttggtgaa ggcgatggcg
     2101 gaccatccgg cgcttctgtg ctgggagagc gtggacgagc ccgcgtggac cggaagcaac
     2161 gcggcggatc tctaccgcgg ctaccgagtc gtccgccgtt tggatccgga ccaccccgtc
     2221 tggatgaacc acgccccgcg caacacggtg gacgagctgg cgctcttcaa ccacggcgcg
     2281 gatgtcgccg gtgcggacat ctatccggtg cccgagccgc agacgcagtc ggatttgccc
     2341 aacaagacga ttgccgtggt cggcgacgaa acgagcaaga acgtcctcgc cgtgctgaac
     2401 cgcaagccga tcttcatggt gctccagggc ttcgcgtggg ctgatctcag ccgcaggcgc
     2461 gggggcacgg agaagccgat ctacccctcg ttccaacagt cgcgcttcat ggcctaccac
     2521 gccatcgtcc gtggtgcccg cggtctcctt ttctggggcg tgaactacac tccgaagccg
     2581 tcgccgttct ggaacagtct ccgccagctc gcctacgagc tatcgcagat gcatgacgtg
     2641 ctggccggcc ccgacacggg ggaggtggcg aaggtccacg ccggcgggaa ggcgattgaa
     2701 acgctgattc ggcggcactg cgagaccgac cagcgcttca tcatcgcggt gaacgcctcg
     2761 cccgagccag tggatgcccg catcgagatc ccgcgcgcca tgccgaaggt gagctggcgc
     2821 gtgctcttcg aggaccggcg catcaccggg aatccgattg tggaccactt cgagccgtgg
     2881 gacgttcaca tctacaccgg gtcgctccgc ttcccgcagg agaagcggct cgagctgcct
     2941 ccggtgacgc aggtgcagcg tccgcgcccc gaggatctcc tcgagcgggg aaacctgttg
     3001 gtcaaccccg gcttcgagtt cacggaggat ggcgaggagt ctcccatcgg ctggtcgctg
     3061 cgccctgcct tctacggcgg cgtctctcgt gaagtgaagc gcagcgggcg ctacagcgtc
     3121 ttcttgaagg ggcgcgatgc cacttccaag cccctctggc tccagacggg gatcgcgacc
     3181 gagacgggcc ggcggtatcg cttcaccgcc caggtaagga ccgagcctgc cggcgcggac
     3241 tgccggatct acgcggagtg gacagatgcg gccggcaagt acttaggcgg aagcgtgccc
     3301 gcgcagtgga gcgccggcac ggcgcagtgg cagccgctca ccctcgattt catggtgaac
     3361 caccccgacg ccaagaagct gtacgtcgtc ctccaggtgc gcggccaggc acaagcgtgg
     3421 ttcggcgacg tgaaactcga ggagatgcgc tgagaaacac gcgggccgcg cccggcccag
     3481 gcatcgcgcg gaggtgaacc tccgcgctgc ttgggagcga agccctgccg ggctgggcca
     3541 ggctacgtgc ccggccagcc tatacaggcg cgggctggga ggcggagccc cgaaggggct
     3601 ttgcctggaa gcagcgcgga gcttccagct ccgcgcgcga gtcttcatcc gtggtaccac
     3661 ccagcggcca ctcgggccac tactccagcc cggccagcgc gcggaactcc tcttcggtca
     3721 gaacggtgat gcccagcacc tgcgccttct ccagcttcga cccggcgccc tcgcccgcga
     3781 cgacatagtc ggtcttcgcg ctgacgctcc ccgtggcgcg cccgcccagc gacttcacca
     3841 gcgcctcggc atcggggcga gtcatctggg ccagggttcc cgtgaagaca aacgtctttc
     3901 cggcgatggg attgtctgca ggtgccgccg gaactgcctc gggcgcctgg ggctgcacgc
     3961 cggccgcgcg aagcctctcc agaagctggc gtgtctgcgg gtctgcgaag aagcgggcca
     4021 cgctcgcggc gatctcgggg ccgatgccgg gcacttcccg gatctcctcc tcgctcgcgg
     4081 cttccagcgt atccaacgtg tggaagtgcg tggcgagaag ctcggcgacg tgctcgccca
     4141 cgtggcggat gcccagggcg aagatcaacc gggcgagggg gacctgacgc gatgcctcga
     4201 tgttgcgcag gatcttttga gccagcgtct tccccatgcg ctccaggggc agtaactgct
     4261 cgagcgtgag cgcgtaaaga tcggccgggt cctttacgag gccccggtcc accagctgct
     4321 cgacctgcgc cgggccgatc ccctcgacgt tcatcgcggt ccgcgagacg aagtgctcga
     4381 tgcgccgctt tgcctgcgcc gggcaggcga cgcccgtgca gcgcgcgacc gcctcgccct
     4441 cggggcgctc cactggcccg ccgcagaccg ggcattctct cgggaagaca aacggctttt
     4501 cggcgccagt tcgcttgctc gtcaccacgg agaccacttc aggaatgaca tcgccggcgc
     4561 gctggatcac cacggtatcg cccacgcgca catctttgcg ccggatctca tcctcattgt
     4621 gg
//
LOCUS       DAOSCV010000270         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11782_length_4616_cov_3.405174, whole genome shotgun sequence.
ACCESSION   DAOSCV010000270 DAOSCV010000000
VERSION     DAOSCV010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11782_length_4616_cov_3.405174"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..646
                     /locus_tag="PLY56_07920"
     CDS             329..646
                     /locus_tag="PLY56_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21447.1"
                     /translation="MITFLILLTVLALSMVLLARALGRMDRNVAPPVPVEEPSAPEQT
                     GLGDPEWNIVIRHQCEPINCPNLKGNPRQPGKEPGCQVCAALFLERYGERLLNEQREG
                     RNR"
     gene            complement(757..972)
                     /locus_tag="PLY56_07925"
     CDS             complement(757..972)
                     /locus_tag="PLY56_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21448.1"
                     /translation="MDFPKERMEIVSHHGGKESEEGWLVLNLGPFDLEGITKIYVNLD
                     DIADIRRHGERRAEVLEQMRSLLGCDD"
     gene            1165..2760
                     /gene="lnt"
                     /locus_tag="PLY56_07930"
     CDS             1165..2760
                     /gene="lnt"
                     /locus_tag="PLY56_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOJ21449.1"
                     /translation="MRRGIVMGALGAVATGAMLAALFPNVGASGLAWVALVPLILAAR
                     ASTPRGAACQRFAAGVLFFLILIEWIRLFDTTAWIAASLVLAAYVALFALLVRLLAPA
                     PTGWRALLVPASAWTALEWARGLGTFGFTWGGLAYSQHGNLPLLQLASVTGPYGITFL
                     IAAVNAGLASMIGVAWSGKPLAGREGVSPRSGDLRGTLARLGVVVAAVGLCAAWGYYR
                     LHRDAGAAPKVRVAVLQGALGWEGDRLPRVTDAMRESSEVYLRLTEEAARQGAQIVIW
                     PESSVPGQVELDPGLRPLREALVATSTRLRVSLLVGSSHRDEAGRLLNSTFLYTPDSG
                     LAGRYDKVHLVPYGEFTPWRQPLDFLYRHFPVIDHEFHPGKGHFPLRAGEAPVGVAIC
                     FESAFPGIARALRRAGARLLVVMTNDAWFGERSATWQHYHMAAFRAVESGAAVARAST
                     SGITALIDPYGRAITETRLFERTLRVGDLPLAQRPTPYQLAGDVIAWLSALAILLLLG
                     IQWRRSRRSMAGLQGPATPKAEA"
     gene            complement(2840..>4616)
                     /locus_tag="PLY56_07935"
     CDS             complement(2840..>4616)
                     /locus_tag="PLY56_07935"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOJ21450.1"
                     /translation="LDFIHSKREAGKITNANISVGITDDFMAAVEADREWELLFPDTS
                     DPDYDTEWDGNLEAWRAKGKPVIVHKTRPAREIWNAIIESAWASAEPGVFFVDRYNRM
                     SNSWYYAPIMCTNPCGEQGLPAWGVCNLGAVNLARFVADGEVQWAPLGQAIRYAVRFL
                     DDVIDATPYFFEENERQQKSERRIGLNTMGLAEMLIRLGIRYGTAESERFIDRLYEFI
                     AREAYTASAELAMEKGVFPAFDAEKFLRSGYMQAMPESIRSMVREKGMRNVTLLTQAP
                     NGTIGTMVGTSTGIEPFYSWTYLRKSRLGVHEENVAIVREWQESNPGEPLPDFFVTAM
                     DLTPEEHVRVQAAVQRWVDSSISKTCNVPNSYTVEQTRRLYELMYRLGCKGGTIYRDG
                     SRDEQVLLRKEKEPEKAPAAVPAPAPDVPKVRPRPYKRAGVTVSKKTPAGTAHITMNN
                     DEDGRPFEVFVDIGKGGTDVKAMAEAIGRLMSLVLRIASPLSPLDRIREMVNQLKGIG
                     GARWIGFGRDRVRSLPDAVAQALEEHYGVEGNGNGHVVPDELSDPIADTTTSIAADLC
                     PACGQAAFVHEEGCMTCHACGHSEC"
BASE COUNT          701 a         1510 c         1561 g          844 t
ORIGIN      
        1 aggcgagcgc caaccccaac tggcacggtc tctgcaagga cccgagtcag ccgctgaagg
       61 ggagcgccgg cgagagcctg cgtcagaccg caccgatgcc ggcaaaacgg tggcggcccg
      121 ccgagtttag ccgattccgc ccccgggtat atggcaacca actctaaggg agggagatcg
      181 ggggatagcg aggagaggcg ctgccgtcag gtgaggagag ccgccgatct ggggtgagaa
      241 gggcctcgca tctttcttgg atgcgcccgt gggatcagtg ccggaccgct aacgaaaggg
      301 gcgatggggt tgagggggga gcgcgacgat gataacgttt ctaattctac tgacggtgct
      361 ggcactgagt atggtcctgc tggcgcgtgc cctgggccgg atggaccgga acgtcgcgcc
      421 gcctgtgccg gtggaggaac cgagcgcgcc agagcagacg ggcctgggcg atccggaatg
      481 gaacatcgtg atacgccacc agtgcgagcc aatcaactgc cccaatttga aagggaaccc
      541 gcggcagccc ggcaaagaac ccggctgcca ggtttgcgcg gctctcttct tggagcgcta
      601 cggggagcgc ctcctcaacg agcagcgcga gggccgcaac cgctagatgt atcgggccgg
      661 atcgaccagg ggggcttggt cggccggccc aaagcctgtc ccctctcagg gcttcgccgc
      721 gtgaagcctg cccccggtgc acggtcgccc gcgccatcaa tcgtcgcatc ccaggaggga
      781 gcgcatctgc tctagcactt ccgcgcgccg ctcgccatgc cttcggatat cggctatgtc
      841 gtccagattg acgtagatct tagtgattcc ctccagatcg aacggcccga ggtttagcac
      901 cagccatccc tcctcggact ccttgcctcc atggtgactg acaatctcca tccgctcttt
      961 ggggaaatcc atcgtagtcc tccttaacgt cccctgaggc ggtattccac accgtcgccc
     1021 ctttcccttt ccggagtcgc atctggatta cagcactccc ccgcgggagc ccggcccatc
     1081 cgtgtccgtc gccctcatcc gcggtattga tgcccttcgc tcgcgcttgt tccatctgcg
     1141 gggcaatggt ataatgcacc tgtgatgaga cgggggattg tgatgggggc gctgggagcg
     1201 gtggcgaccg gcgccatgct cgcggcgctc tttccgaatg tgggcgcgtc cggcctggcc
     1261 tgggtcgcgc tcgtgcccct gatcctggcg gcccgggcgt ccaccccccg aggcgcggcg
     1321 tgccagcgct tcgccgccgg cgtgctcttc ttcctcatcc tcatcgagtg gatccgcctc
     1381 ttcgatacca cggcctggat tgccgcgtcg ctggtcctcg ctgcctatgt ggcgctcttc
     1441 gccctgctcg tacggctgct ggcgcccgcg ccgaccggtt ggagggcgct cctggtgccc
     1501 gcgtccgcct ggacagctct ggagtgggcg cgcggcctgg ggacgtttgg gttcacctgg
     1561 ggcggcctgg cctattcaca acatggcaac ctaccgctgc tccagctggc aagcgtgacc
     1621 ggcccctatg gcatcacctt cctgatcgcg gcggtcaacg ccgggcttgc ctcaatgatc
     1681 ggcgtggcgt ggtcgggcaa gccgctcgcc ggacgagaag gcgtctcgcc acgctccggc
     1741 gacctgcgcg gcacgctcgc ccgccttggg gtggtggtgg cggctgtggg tctctgcgcg
     1801 gcctggggat attaccgact gcaccgggat gcgggtgcgg ccccaaaggt gcgggtcgcg
     1861 gtgcttcagg gcgccctggg ttgggagggc gaccgcttgc cgcgcgtcac cgacgcgatg
     1921 cgggagagca gcgaagtcta cctgcgcctg acggaggaag cggcgcgcca gggggcccag
     1981 atcgtgatct ggccggaaag ctccgtgccg gggcaggtgg agctggatcc ggggctgcgg
     2041 ccgctccgcg aggcgctcgt ggctacgtcc acgcgcctgc gcgtgagtct cctggttggc
     2101 agctcgcata gagacgaggc gggacgcctg ctcaactcca cgtttctcta cacgcccgat
     2161 tccggcttgg ccgggcgcta cgacaaggtt cacctggtgc cgtatggcga gttcacgccc
     2221 tggcgccagc ctctcgattt cctctaccgc cacttcccgg taatcgacca cgagtttcat
     2281 ccgggcaagg ggcattttcc cttgcgggcg ggcgaggccc ccgtgggcgt ggcaatctgc
     2341 ttcgagtcgg cctttcccgg gattgcccgt gcgttgcgcc gggccggcgc gcgcctcctg
     2401 gtggtgatga cgaacgatgc ctggtttgga gagcgctcgg ccacctggca gcactatcac
     2461 atggccgcct tccgcgccgt cgagagcggg gcggccgtgg cccgagcctc gacatccggg
     2521 atcacagcgc tcatcgatcc ctacgggcgc gcgatcacgg agacgcggct tttcgagcgc
     2581 acgcttcgcg tgggcgatct cccactggcg cagagaccga cgccctacca gttggccggc
     2641 gacgtcatcg cctggctcag cgccctggcc atcctgctcc tcctgggtat ccaatggcgc
     2701 cggtcgcgcc ggagcatggc cggcctccag ggcccggcaa cacccaaggc ggaagcctaa
     2761 atcgggcgcg caccccggag aataagagag ccgccctgcc cggcggtggg gcagagcggc
     2821 tctcaacggc ctggctagat tagcactcgc tatggccgca cgcgtggcag gtcatgcacc
     2881 cctcctcgtg gacgaacgct gcctgcccgc aggcggggca gaggtcggcc gcgatggatg
     2941 tggtggtgtc cgcaatcgga tcggagagct cgtcgggcac gacgtggccg ttcccgttgc
     3001 cttccacgcc gtaatgctcc tccagggcct gggccactgc gtcggggagc gagcggacgc
     3061 ggtctctgcc gaagccgatc cagcgggcac cgccgatgcc cttcagctgg ttgaccatct
     3121 cgcggatgcg atccagcggc gagaggggcg aagcgatccg gaggaccagc gacatcaggc
     3181 ggccgatcgc ctccgccatc gccttgacat cggtgccacc cttgccaatg tccacgaaga
     3241 cctcgaaggg gcgcccatcc tcgtcgttgt tcatggtgat gtgcgcggtg ccggccggcg
     3301 tcttcttgct gaccgtgacg ccagcgcgct tgtagggtcg ggggcgcacc ttgggcacat
     3361 ctggagcggg agctggcacg gcggcgggcg ccttctcggg ctccttctcc ttgcgcaaga
     3421 gcacctgctc atcgcgcgag ccgtcgcggt agatcgtgcc gcccttgcaa cccagccggt
     3481 acatcagctc atagagtcgg cgcgtctgct cgacggtgta gctgttgggc acgttgcacg
     3541 tcttagagat gctggaatcg acccagcgct ggacggcggc ctgcacgcgc acatgctcct
     3601 cgggggtgag gtccatggcg gtgacgaaga agtcggggag tggttctccc ggattgctct
     3661 cctgccactc gcgcacaatc gcgacgttct cctcatgcac gcccaggcgg ctcttgcgga
     3721 ggtaggtcca ggagtagaac ggctcgatac cagtggatgt gccgaccatg gtgccgatgg
     3781 tgccgttggg ggcctgggtg agcaacgtca cgttgcgcat ccccttctcg cgaaccatgc
     3841 tgcggatgct ctccggcatc gcctgcatgt agccgcttcg caggaacttc tcggcatcga
     3901 aagcggggaa aacgcctttc tccatggcga gctcggcaga ggcggtgtag gcctcgcgag
     3961 cgataaactc gtagagccgg tcaatgaacc gctcggactc ggccgtgcca tagcgaatgc
     4021 ccaggcggat gagcatctct gccaggccca tggtgttcag gccgatgcgg cgctccgact
     4081 tttgctgtcg ctcattctcc tcaaagaagt agggtgtggc atcgatcacg tcgtcgagga
     4141 agcgaacggc gtagcggatc gcttggccca ggggagccca ctgaacctcg ccatcggcga
     4201 cgaaccgggc taggttgacc gcgccgaggt tgcacacgcc ccaggcggga aggccctgct
     4261 cgccgcaggg gttggtgcac atgatcggcg cgtagtacca ggagttggac atccggttgt
     4321 agcggtcaac gaagaagact cccggttcgg cgctggccca ggcgctttcg atgatggcgt
     4381 tccagatctc gcgtgccggg cgtgtcttgt gcacgatgac cggcttgccc tttgcccgcc
     4441 acgcctcgag gttgccatcc cactcggtat cgtagtcggg atcgctcgta tcggggaaga
     4501 gcagctccca ctcgcggtct gcctccacgg cggccatgaa gtcatcggtg atccccacgc
     4561 tgatgttggc gttggtgatc ttgccggctt cgcgcttgga gtggatgaaa tcgagc
//
LOCUS       DAOSCV010000271         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11783_length_4616_cov_3.232186, whole genome shotgun sequence.
ACCESSION   DAOSCV010000271 DAOSCV010000000
VERSION     DAOSCV010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11783_length_4616_cov_3.232186"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>543)
                     /locus_tag="PLY56_07940"
     CDS             complement(<1..>543)
                     /locus_tag="PLY56_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21451.1"
                     /translation="LAMLTFLSRGFPVAFSGYQVDSLRAAHQTSEKPRRFIIGLTIAL
                     IAGLALAFYFHLSGYYRVGAQQVRNGIWGWSMATMEYDTAINAATSPRPMDKPRVMAG
                     VTGFGIATVLQMLRTRIVGFPLHPLGYAVGNAFGSLLWWPFFLVWLVKVAVLRFGGMP
                     LYRQTLPGFLGLALGHFFTAG"
     assembly_gap    544..553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<554..1923)
                     /locus_tag="PLY56_07945"
     CDS             complement(<554..1923)
                     /locus_tag="PLY56_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21452.1"
                     /translation="MAMPQHTVSDPMDERLEPGQADSPERRGAVTPRAILTGLFLTVV
                     AGFWIRESEIVALGVYITESVPTIPAVAAILLVLLANTLLTYTPWADRRFSRGEMLFI
                     FFFVCVSTMSFSCGVARFFLALMTAPFYFHHTGNKLEEAQKYLPEWATVRDPDAIRGV
                     YEALRGAPIPWGVWVGPLLAWIGFFVALWLAMMCLVMLMHRPWVEKEKLTFPLVQLPL
                     EITDTEPRSGGIPAFFRNPVMWTGFAIAFVYNLANMIHAVAPSFPAWKNSFSFIPFTT
                     PPFNAVGGLTIYNRPELIGFGYLVSAEITFSIWATFLLERLAAVVMSGMGYREAGMPF
                     MQEQSFGAYIAMALLLLYLGKGHLISIARAAVSSAEPEMRGYRWAFWGFWLSVIALLV
                     FCKMLGMMWWVGALYLGIFLAIALTYARVRAETGIPLVWCFPFGMPKTMLFSILGTAP
                     LAPGGDP"
     gene            2180..2812
                     /locus_tag="PLY56_07950"
     CDS             2180..2812
                     /locus_tag="PLY56_07950"
                     /inference="COORDINATES: protein motif:HMM:NF025013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HOJ21453.1"
                     /translation="MTNTDGKTSAPRNIWQQLARGSVFLLSVAAWGTIIFLMRDLDPI
                     HGPSARRAEVRVLSEMRLYCAHSVALGRLAEAKAIHEEIRQFAGERVVSYSQEVARLD
                     ALLRERHGRMHRAHTREGQDPALARLERLSGAAFETAFLDEALYRNARALQQIESHRQ
                     RIGDTALNYLHDRLCAELRAERKRLEGWRSRWYGSSPTTTPQAASPGEAW"
     gene            complement(2885..3946)
                     /locus_tag="PLY56_07955"
     CDS             complement(2885..3946)
                     /locus_tag="PLY56_07955"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21454.1"
                     /translation="MATKPLRIGVAGLGRIGWSFHCKTLAAHPDFELVAVQDIEPERR
                     QEAERTYGVTAYAEFADLLRHADLDVVTIATPTHLHHDMALDALSAGHHIVIEKPMAQ
                     EVSEAEAIASEARRRGRLVTVYQPHRVMAYFQHLTRILQAGTIGEVYHVRRALFNFAR
                     RNDWQSLQRYGGGMLNNYGAHCIDQVLQLIGYDIRRIFCNLRLVASLGDADDVVKILI
                     ETHKGVLGEIDINQASAIRPYELQVWGAHGAVTLERNTFTVRYFDPAELPEKALDPGL
                     ASANRAYPSDNVPFREESFPVDPSLAVDFYGNLASAIREGTPLLVPPEETLAVMRTIE
                     RCREDAGRILETRIASERP"
     gene            4191..>4616
                     /locus_tag="PLY56_07960"
     CDS             4191..>4616
                     /locus_tag="PLY56_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21455.1"
                     /translation="MNQAAGPGDFPQSFTSNEEADFLVDDDHPRDECGVFGICAPGED
                     VARLAYFGLFALQHRGQESAGIAVSDGRRLKVSKAMGLVTQVFDEEVLQSLRGGFGIG
                     HVRYSTMGSSVLCNAQPVVVETPYGRIAVAHNGNLVNCAP"
BASE COUNT          877 a         1440 c         1522 g          767 t
ORIGIN      
        1 ccggcggtga agaagtgccc cagcgccagg ccgaggaagc cggggagggt ctggcggtag
       61 agcggcatgc cgccgaagcg gagaaccgct accttcacca gccagaccag gaagaagggc
      121 caccagagga gcgacccgaa ggcattgccg accgcgtagc cgagcgggtg cagcgggaag
      181 ccgacgatgc gcgtgcgcag catctggagc accgtagcga tgccaaaccc ggtgacgccg
      241 gccataacgc gcggcttgtc catcgggcgc ggggaagtgg ccgcattgat ggcggtatca
      301 tactccatcg tcgccatgct ccagccccag atcccgttgc gcacttgctg cgcgccgacg
      361 cggtagtagc ccgagaggtg gaaatagaac gcgagcgcca ggccggctat cagggcaatg
      421 gtgagcccga tgatgaagcg ccttggcttc tcggaggtct gatgcgctgc gcgcaggcta
      481 tccacctggt agccagagaa ggcgaccgga aatccgcgcg agaggaaggt gagcatcgcc
      541 agtnnnnnnn nnnggatcgc ccccgggcgc cagcggggcg gtccccagta tggagaagag
      601 catcgtcttg ggcattccga aggggaagca ccagacgagc gggatgcccg tttcggcgcg
      661 gacgcgcgcg taggtgagcg cgatcgccag gaagatgccc aggtagagcg cgccgaccca
      721 ccacatcata ccgagcatct tgcagaagac caggagcgcg atcacgctga gccagaatcc
      781 ccagaaggcc caccgatagc cgcgcatctc cggctctgcc gacgacacag cggcgcgcgc
      841 gatgctgatc agatgcccct tgcccagata gagaaggagc agcgccatgg cgatgtaagc
      901 accgaagctc tgttcctgca tgaagggcat gccggcctcg cgatagccca tgccgctcat
      961 caccacggcc gccagacgct cgagcaggaa ggtggcccaa atcgagaagg tgatctccgc
     1021 cgaaaccagg tagccaaacc cgatcagctc cggacggttg tagatggtga ggccgccgac
     1081 cgcgttgaat gggggcgtcg tgaaggggat gaacgaaaac gagttcttcc acgcggggaa
     1141 cgacggcgcg acggcgtgga tcatgttggc gaggttatag acgaacgcga tcgcgaagcc
     1201 cgtccacatg accgggttgc ggaagaacgc cgggatccct cccgaacgcg gctccgtatc
     1261 cgtgatctcc aggggaagct gcaccagcgg gaaagtgagc ttctccttct ccacccaggg
     1321 ccggtgcatc agcatcacca aacacatcat tgccagccac agggcgacga agaagccgat
     1381 ccacgccagc agcggaccca cccagacgcc ccacgggatc ggcgccccgc gcagcgcctc
     1441 gtaaaccccc cgaatcgcat ccgggtcgcg caccgtggcc cattcgggaa ggtacttctg
     1501 cgcttcctcc agcttgttgc ccgtgtggtg gaagtagaac ggggcagtca tcaaggcaag
     1561 gaaaaagcgc gctacgccgc agctgaacga catcgtggag acgcagacaa agaagaagat
     1621 aaagagcatc tctccacgac tgaaacgtcg gtctgcccag ggggtgtagg taagcaaagt
     1681 gttggcgagt aacaccaaca gaattgccgc gaccgcgggg atcgtgggca cgctctccgt
     1741 gatataaact ccgagagcca cgatctccga ctcccggatc cagaacccgg ctacgaccgt
     1801 caggaagagg cccgtaagga ttgcccgggg ggttaccgcg ccgcggcgtt cagggctatc
     1861 ggcttgaccc ggttccagcc gctcgtccat tgggtcgctc accgtatgct ggggcatggc
     1921 catcagcacg ccatcctttc cacttccaat ccaagttgga gtagttctcc cgtatcaatt
     1981 cgccgtgttt cgcggggttc cttgtgggaa agcgcttctc tttcacacgg gcactcagat
     2041 gcttctcgcc tctcccaggg ggtggtcgct tcctcgccca attcacgctc catttcacgc
     2101 gctcggaggc gttctggcat gcatcgtgcg cccgccaggc gatgcggtcg ggcccagagg
     2161 aatgccggag gagaggcgag tgaccaatac ggacgggaag acgtccgctc cgagaaacat
     2221 ctggcagcag ctggcacgcg gatccgtctt cctcttgagc gttgccgcat ggggaaccat
     2281 catcttcttg atgcgtgacc tggatcccat ccatgggcct tcggccaggc gggcggaggt
     2341 gagagtgctc tccgagatgc gcctctactg cgcgcatagc gtcgccctgg gccggctggc
     2401 agaggcaaag gccatccatg aagagatccg acagtttgcc ggagagcggg tagtctctta
     2461 cagccaggaa gtagctcgcc tggatgcgct actccgcgaa cggcacgggc gaatgcatcg
     2521 cgcccacacc cgcgagggcc aagatccggc gctcgcccgt ctggagcggc tgagcggcgc
     2581 cgcgttcgag acggcattcc tggacgaagc cctttaccgc aacgcgcggg cgctgcaaca
     2641 gatcgagagc caccggcagc gcatcggtga taccgcgctc aactatctcc acgatcgtct
     2701 ttgcgcggag ctccgcgccg aacgaaagcg cctggaggga tggcggtcgc gctggtacgg
     2761 cagctcccca accacgacgc cccaggcagc ttcgccgggt gaggcctggt aacccggacc
     2821 ggctatcgac cggcgtatcg tcctctcgcc cggttccggc gagctgcctc ggcgtgccag
     2881 cggattaggg gcgttcgctc gcgatccgcg tctccaggat gcgcccggca tcctcgcgac
     2941 agcgctcgat ggtgcgcatg acggccaggg tctcctctgg cggcacgagg agaggcgtgc
     3001 cttcgcggat agcagatgcc aggttgccgt agaagtcgac cgccaggctc ggatccacgg
     3061 ggaagctctc ttcccggaag ggcacgttgt cgctggggta agcgcgattg gcgctcgcca
     3121 ggccgggatc caacgccttc tccggcagct cggccggatc gaagtagcgc accgtgaagg
     3181 tattccgctc cagggtgacg gcgccgtgcg cgccccacac ctgaagctcg tagggccgga
     3241 tcgcggaggc ctgattgata tcaatctcgc cgaggacgcc cttgtgtgtc tcgatgagga
     3301 tctttaccac gtcgtcggca tcgcccaagg aagcgaccag gcggaggttg cagaagatcc
     3361 gccggatgtc atacccgatc agctggagca cctggtcgat gcagtgcgcg ccgtagttgt
     3421 tgagcatgcc accgccatac cgctgcaggc tctgccaatc gttgcgccgc gcaaagttga
     3481 agagagcccg gcgcacgtgg tagacctcgc cgatggtgcc cgcctgcagg atgcgcgtga
     3541 ggtgctggaa gtaggccatc acgcggtggg gttgatagac ggtgacaagg cgcccgcggc
     3601 ggcgcgcctc actcgcgatc gcctccgcct cgctaacctc ctgcgccatc ggcttctcga
     3661 tgacgatatg gtggccggcg ctcagcgcgt ccagggccat gtcgtggtgc aggtgggtgg
     3721 gtgtggcgat ggtgaccaca tccaggtccg cgtggcgcag caggtcggca aactcggcat
     3781 aggcggtcac gccataggtg cgctccgcct cctggcggcg ttccggctca atatcttgca
     3841 cggccaccag ctcgaaatcg gggtgcgctg cgagcgtctt gcaatggaag ctccacccga
     3901 ttcgacccag tcccgccact ccgatgcgta agggtttggt cgccatttca tcgcctctcg
     3961 ttccgcaggt ttggcgctgc ctggggcagg cccgctctat tgtactggcc ggggcggtgc
     4021 cggtcaagag gagtaaacat tcagtttgag aatgggatcg gggtagaaac tgccgggcat
     4081 ttctgctata atgggcgttg ggagtccctc cagtagcggc atgccgactg cctcgcgcca
     4141 accggggcca gggggggcaa ccgcgcgaac agcttacgtc gggaggaacc atgaaccagg
     4201 ctgccgggcc gggcgatttt ccgcaatcct tcacgagcaa cgaagaagcc gatttcctcg
     4261 tggacgatga tcaccccagg gatgagtgcg gggtattcgg catctgtgcc cctggcgagg
     4321 atgtggcccg cctcgcctat tttggccttt ttgcgctgca gcaccgagga caggagtctg
     4381 ccggaatcgc cgtctcggac gggcggcgcc tcaaagtgag caaggcgatg ggtctggtga
     4441 cccaggtctt tgatgaggag gtactccaga gcctgcgcgg cggtttcggg atcgggcacg
     4501 tccgctattc taccatgggg tcgagcgtcc tctgcaatgc ccagccggtc gttgtggaga
     4561 cgccatacgg ccggatcgcc gtggcgcata atggcaacct ggtgaactgc gcgccc
//
LOCUS       DAOSCV010000272         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11800_length_4612_cov_2.892693, whole genome shotgun sequence.
ACCESSION   DAOSCV010000272 DAOSCV010000000
VERSION     DAOSCV010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11800_length_4612_cov_2.892693"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..999
                     /locus_tag="PLY56_07965"
     CDS             163..999
                     /locus_tag="PLY56_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21456.1"
                     /translation="MAEGEPLQTLQASTAYRSALLVALGYVVVATVWIFLSDLVLLTI
                     PSDSSWLLFVSLLKGWSFVLVTGGLLYVLLRQHARRILDAERAYRIAVERAEQQKRLF
                     FQNTITAITEGKMLVRSPGALAAMPTPPQMEEDVVSAAQASGVREKIREAALAAGIRD
                     ERAADLVVAAGEAITNAIKHANGGRVSVHVGADCVWVRVTDHGPGIADLMLPRVVLER
                     GYSTKPSLGMGYTLMLALSDRLYLTTGPTGTTVVLEMCRREGKAATVAAIPDRTPLVD
                     AL"
     gene            complement(1192..2151)
                     /locus_tag="PLY56_07970"
     CDS             complement(1192..2151)
                     /locus_tag="PLY56_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOJ21457.1"
                     /translation="MPKKIWLIALSVMATMALAAFPARAQEESPERVAVAVMDFTTGA
                     VPWDLRDDNIGRICANALTVLLQRNPYFEVAERARIETVANEIRMGASGLVDPDKAAE
                     IGKLLAVKYLVYGDVQSIEIGEGIASAISGAIGGGILGGKVRVKGTHCRAHVNYKMVE
                     VETGKIVLSRTFKEIMDVTHVVDGPEARLKLVNETLSKTAERMARAMMPKIEAIVAAV
                     NAEDGTVVITAGATHGVVEGAMFRVYRKNNEVKNPVAGEVIDVQITELAKARCTDVRE
                     KASTLQVGDYVKAKRSNKQEWKVLKEKLAEIKVGDLVGALEAE"
     gene            complement(2231..3370)
                     /locus_tag="PLY56_07975"
     CDS             complement(2231..3370)
                     /locus_tag="PLY56_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21458.1"
                     /translation="MRFLERAIMMAALLLVAATGGSEGAERLHRPEKQTVTVKGTAVI
                     EGGNVAAAEKAARADAMRKGIEQVIGAYVSGKAKAENGQLIDSLVTEKFGGFAQIVRV
                     LSQKQEDGMYEIQAEVAVSPVPMLEIMRKNGLMREWRVMVIIPEQHISTPVPDPAAET
                     EFIRQFVNAGYKVIDQKRYAELRQQNASRLKDPAVAAEIGRKMGADIVITGEAFSQRV
                     VPDPAPGFVSCRARVEVRAILADTAEIVAADATEGPGADLVESVSAKKALQNTAKKLA
                     PLFVRDITLLPASESRNIQVEVSVFASFAKAARFERALKGLPGVTKVSRESYDEGVLV
                     LNVESDTESAEDFALALEEAEAMKPFGLTVGAVSKTRIRATVKQE"
     gene            complement(3548..4579)
                     /locus_tag="PLY56_07980"
     CDS             complement(3548..4579)
                     /locus_tag="PLY56_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21459.1"
                     /translation="MRLSKVLCVGALAAAVSAVPVQAARRDVAVGVQQHVTVNVNLGQ
                     SGVPHRINGLVDYGNNLVYIKTEGLPKEGVRSKAQKRWTAREAAGLYAYRDLALMVVG
                     MMVGGRIGAKEGALVESMVAGAFKAKVRGGKIYDDPNDPKMRALGLAKDLGWNRDDEV
                     YTIVMEARLYGAESVSDAWCSEQVQQALAKIEEMKPQQAAAPGGLEEATPTAPVQYSN
                     VEPVKSAPALGAKTGVIVLCDGLGVQAGLAPKIFRGEQGDAIFGANVPTKVAVEMGAV
                     GYAASEAEARKNSRVGSDPIVIKGIKKRGNYGVAISEEDADMLLKENEKGKFLDSFSV
                     VFVVKDDVE"
BASE COUNT          773 a         1672 c         1296 g          871 t
ORIGIN      
        1 caccagcact ccctggcctc gggtctgcgt tactcctccc gaggccccgg ggatctttca
       61 gacggttagc cgcacgccct ggcgggtaga atcccggcga gagtggctgc gcgaggcaac
      121 gcgccgccag tggatgtgac caccgggaag ggacccgcgc ccgtggctga aggcgagccc
      181 ttacagaccc tgcaagcgag caccgcgtat cgctcggcgc tgctagtggc gctgggttac
      241 gtggtagtgg cgactgtctg gatcttcctc agcgacctgg tgctcctcac catcccatcc
      301 gattcctcgt ggctgctctt cgtgtcgttg ctgaaaggct ggagtttcgt cctcgtcact
      361 ggtggcctgc tctacgttct actccggcaa catgccaggc gcattctgga tgcggaacgc
      421 gcctatcgga tcgccgtcga gcgggcggag cagcagaagc gcctcttctt ccaaaacacg
      481 atcacggcaa tcaccgaagg caagatgctt gttcgcagcc ccggagctct tgcggccatg
      541 ccgacgcccc cccagatgga ggaagacgtc gtttcggcgg cgcaagcatc cggagtgcgc
      601 gagaagatac gggaagccgc cctcgccgct gggatccgtg acgaacgcgc cgccgatctc
      661 gtcgtcgccg ccggcgaagc gatcaccaac gcgatcaagc acgccaatgg cgggagagtc
      721 tctgttcacg tgggagccga ttgcgtttgg gtgcgcgtga cggatcacgg cccggggatc
      781 gccgacctga tgttgccgcg cgtcgtcctc gagcgtggct actccacaaa gccatcgctc
      841 ggcatgggct acactctgat gctcgctctc tccgaccggc tctacctgac aaccggcccc
      901 accggcacca cggtcgtcct cgagatgtgc cggcgagagg gcaaagccgc caccgtcgcc
      961 gccattccgg atcgcacacc tctcgtcgac gcactgtagc ggtccctggc aggggcacac
     1021 gggtgccgga ggcgcaccac tgctaccgtt ccgccctcga agcacacggt cggcccagtc
     1081 aaagcccttc ttgccagagg catcctgatc agttcggaga taaaagcgcg gggaggacac
     1141 ctgggtgccc tccccgcgcc tcgaagtcgc gaaactgctg cactacaagg ttcactcggc
     1201 ttcaagcgcg cctacgagat cgccgacctt gatctcggcc agcttctcct tcagcacctt
     1261 ccactcttgc ttattgctgc gtttcgcctt gacgtagtcg cccacctgaa gggtgctcgc
     1321 cttctcgcgc acgtcggtgc agcgcgcctt cgccagctcg gtaatctgca cgtcgatcac
     1381 ctcgccggcg accgggttct tcacctcgtt gttcttgcgg tagacacgga acatcgcgcc
     1441 ttcgaccaca ccatgcgtgg cgccagcggt gatgaccacc gtgccgtcct cagcgttgac
     1501 ggcggccacg atcgcctcga tcttcggcat catcgcccgc gccatccgct ccgcggtctt
     1561 cgacagtgtc tcattcacca gcttgagccg ggcctcgggc ccgtccacca catgggtgac
     1621 atccatgatc tccttgaagg tgcgcgagag cacgatcttg ccggtctcca cctccaccat
     1681 cttgtagttg acgtgggcgc gacaatgcgt gcccttgacc ctgaccttgc ctcccaagat
     1741 gcccccgcca atggcgccgc tgatcgcgct cgcgatcccc tcgccgatct cgatgctctg
     1801 gacatcgccg tagaccaggt acttgaccgc gagcagcttg ccgatctcgg cggctttgtc
     1861 tggatcaacg agaccgctcg ctcccatacg gatctcgttc gccaccgtct cgatccgcgc
     1921 gcgctcggcc acctcgaagt aggggttgcg ctggaggagc accgtcagcg cgttggcgca
     1981 gattcgcccg atgttgtcgt cgcgcaagtc ccacggcacc gcgcccgtgg tgaaatccat
     2041 cacggccacg gccacccgct ccgggctctc ctcctgggcc cgggcaggaa acgccgcgag
     2101 ggccatcgtc gccatcactg ataaggcgat cagccagatc ttctttggca tccaacactc
     2161 cttaccgcca cgatggatcc gtcaacacac ccctgccaca acgcacccgt cccatcgcga
     2221 ggcacttccg ctactcttgc ttcactgtcg cacggatgcg cgtcttgctg accgcgccca
     2281 ccgtaaggcc gaatggcttc atcgcctcgg cctcttccaa tgcaagcgcg aagtcctcgg
     2341 ccgattcggt atcagactcc acgttcaaaa ccagaacgcc ctcgtcatac gactcccgac
     2401 tcaccttggt cacgccgggc aggcctttca gcgcccgctc gaaacgcgcc gcctttgcga
     2461 aggaagcgaa gacgctcacc tcaacctgga tgttgcggct ttccgaggcg ggaagcagcg
     2521 tgatatcccg gacaaagaga ggcgccagct tcttcgccgt gttctgcagc gccttctttg
     2581 ccgagacgga ctccacgagg tccgcgcccg ggccctcggt ggcatccgcg gccacgatct
     2641 ctgcggtatc cgccagaatc gcgcgcacct cgacgcgcgc gcggcacgag acaaagccag
     2701 gcgccgggtc cggcaccacc cgctggctga aagcctcgcc ggtgatcaca atgtcggcgc
     2761 ccatcttgcg accgatctcc gcggccacgg ccgggtcctt caggcgcgat gcgttctgct
     2821 ggcgcagctc cgcgtaacgc ttctggtcga tcaccttgta gccggcgttc acaaactggc
     2881 gaatgaactc cgtctcggcc gccggatcgg gcacgggcgt cgagatatgc tgctcgggga
     2941 tgatcaccat cactcgccac tcgcgcatga gaccattctt gcgcatgatc tcaagcatcg
     3001 gcaccgggga gaccgcgacc tcggcctgga tctcatacat cccgtcctcc tgcttttgcg
     3061 agaggacgcg cacgatctgc gcgaagccgc cgaacttctc ggtaaccaga ctgtcgatga
     3121 gctggccatt ctcggccttc gccttgccag agacataggc gccgatgacc tgctcgatcc
     3181 ccttgcgcat ggcgtcggca cgcgccgcct tctctgccgc cgccacattg cccccttcga
     3241 tcaccgcagt gcccttcacg gtaaccgtct gcttctcggg ccggtgcagg cgctccgccc
     3301 cctcgcttcc accggtggcg gcgaccagca gcagggcagc catcatgatc gcacgctcta
     3361 gaaaacgcat ccgttctccc tcgctcaaac gccgcggcgg caacctcgct ttcgatagcc
     3421 tcaggcacgc caccgtggga cgttggcagc atacgccagg cacgcgcgct ggccgtgccc
     3481 tgttaccaag acaaccgggc gtcgcagtca gttgccaccg gctgcgacgc cctctgtaga
     3541 ctagccctta ctctacgtca tccttgacga cgaagaccac ggagaagcta tccaggaact
     3601 tgcccttctc gttctccttc agaagcatgt cagcgtcttc ctcgctgatg gcgactccgt
     3661 agttgccgcg cttcttgatc cccttgatca caatcggatc gctgcctacg cgggaattct
     3721 tgcgcgcttc cgcttcgctc gccgcatagc cgacggcgcc catctcgacg gcgaccttcg
     3781 tcggcacgtt ggcaccaaag atggcatcgc cctgctcgcc gcggaagatc ttcggcgcga
     3841 ggccggcctg aacacccagc ccatcgcaga gcacgatcac gcccgtctta gcgccaagcg
     3901 cgggcgcgga tttcacaggc tccacgttgg agtattgtac gggcgcggtg ggagtggctt
     3961 cttccaaacc tcccggcgct gccgcctgct ggggcttcat ctcctcgatc ttcgcgaggg
     4021 cctgctgcac ctgctccgag caccacgcat ccgagacgct ctccgcgcca tacagccgcg
     4081 cctccatcac gatggtgtag acctcgtcat cgcggttcca tcccaggtct ttcgccagac
     4141 cgagcgcccg catcttcgga tcgttgggat cgtcatagat cttcccgccg cgaacctttg
     4201 ccttgaacgc gccggccacc atcgattcga cgagcgcgcc ctccttggcg ccgatgcgcc
     4261 cgccaaccat catgcccact accatcaacg ccaagtcacg gtaggcatag aggccggcgg
     4321 cttcccgcgc ggtccaccgc ttctgagcct tggagcgcac gccctccttg gggagaccct
     4381 ccgtcttgat ataaaccaga ttgttgccat agtcgaccaa gccgttgatt cggtgcggca
     4441 cgccactctg gccaaggttc acattcaccg tcacgtgctg ctgcacgcca accgccacat
     4501 cacggcgggc ggcctgcaca ggcacggcgc tgacagccgc cgccagggcg cccacgcaca
     4561 acactttgct gagcctcatc tcgcaatctc tccttccagc cgcctatcct gg
//
LOCUS       DAOSCV010000273         4607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11821_length_4607_cov_3.448155, whole genome shotgun sequence.
ACCESSION   DAOSCV010000273 DAOSCV010000000
VERSION     DAOSCV010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11821_length_4607_cov_3.448155"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..1275)
                     /gene="alr"
                     /locus_tag="PLY56_07985"
     CDS             complement(151..1275)
                     /gene="alr"
                     /locus_tag="PLY56_07985"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ21460.1"
                     /translation="MEELATAQEHTAWVEVRRDALAHNLAQVRAHIGPDVRLLAVVKA
                     DAYGHGAVEASRTFLDAGADFLGVTRVEEALELRHAGIDAPILVFGAALPNQARVVVA
                     HRLSQTVCTLESARALSAAARASGATAQVHVKVDSGMGRIGIRCAELLEFVRALHALP
                     NLEVQGIYTHFATALERELTFVRKQLSLFLRAIAEVEKAGLGRPLIHAANSAAILRLE
                     ASHLDMVRPGTILYGQYPSAHVPRILELRPAWQLKSRVAFVKQVQRGQSIGYGRDFLA
                     PREMTVATIPLGYADGLTLTPVRTSRSLSELARTAREFARRGRMGVRVHGQHAPFVGR
                     VAMQMCCIDVSHIPNVEVGDEVLLRARFTLTNPRIPRIYC"
     gene            complement(1345..2646)
                     /locus_tag="PLY56_07990"
     CDS             complement(1345..2646)
                     /locus_tag="PLY56_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ21461.1"
                     /translation="MIKAGIRIAVPLLMLLLSVSHAHALYSYSDVVSSLEELCASASS
                     PGGPQMRVVELGTTLKGKPILMAVLSAAPVEDPKSSDDVEEPAEEPAEAPAATAPEPG
                     VDAPESDATEPAVLTLTLDPVDEAPAAVEAAIAAPPAGEDRPRRVFILCRQHGNEPAS
                     TEGVMRFLKEYATTEDPAKLEILRRVTFLVVPMLNVDGVEKDQRRNARNVDLNRDWLS
                     QSQPETRAIVQAVRKWRPDLLMDLHELHPTDQTPSFVEAMEPEAGARGGVGAACNAAI
                     AAVIGPLREQSIRIYARPVFNYREPRLAHRYFAVKEGVPAILVETRRAWGLALEHRTV
                     VHYAAIVAAARYMAGYELTTPSRVIAEWLEPSANTAYRTAVAKRSGSGGDDKVAEAIA
                     KRQRAQLASRKGKPASVDKKRAELIKKRAAQQKKKTPAKKK"
     gene            3146..>4607
                     /locus_tag="PLY56_07995"
     CDS             3146..>4607
                     /locus_tag="PLY56_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ21462.1"
                     /translation="MREELETYREAVRRDPEDGPARLRLALALLDQGGPDAESSEELL
                     AELREAVRLCPDNPLAHTMLGVVLRGKGLREEAVSAWEQAARLAPEDPVVHAFLGDAL
                     CEAGRYADAVPHWRQAVRACPENAEYRDNLVIALARDIETTAGDAAERRARAQERLAA
                     EPDAPEMHAVLGWLLSQERQLTEAIHHWREAIRLGGGTAGIHNNLGLALFYTGAIAES
                     IDEFREAARLEPSLAASHTNRGTALRALGRSREAVDAFREALALDPEETTARHYLADL
                     LAELNEPDEAISHYREVIRRMPQRAPAYRGLGDALDQLGRVEDAMAAYRQALALDRDD
                     PVARYHLAVALDRTGRQEEAIPEYRETIRLCPNHAGAHNNLGVLLFERAKDLPAGPER
                     DAGLKAADEHYCAALRANLGDAEALFNLGEVLSLTGRPAEAVVAFRQASRLNPDDAET
                     QAGLAAALEATGAREEAADAWSRVLASGDAVLAAHAE"
BASE COUNT          738 a         1524 c         1574 g          771 t
ORIGIN      
        1 accctgcgcc ccagtataac agccgttcct gcatgtgtca accgcttttc cagcgtgcgt
       61 ttcacctgcc gcgcagcgaa ggcgttcctc gccgggggat cgcgggatct gggcgccctc
      121 tcagggggct cgattcttca ggggaaaggg ttagcagtag atgcggggga tccgcgggtt
      181 ggtgagcgtg aagcgggcac gcaggagaac ctcgtcgccc acctccacgt ttggaatgtg
      241 gctcacgtcg atgcagcaca tctgcatggc gacgcggcca acgaagggag cgtgttggcc
      301 gtggacgcgc actcccatcc gcccgcggcg cgcgaactcc cgcgctgttc gtgccaactc
      361 gctaagcgaa cgtgaggtgc gaaccggggt gagcgtgagg ccatcggcat agccgagcgg
      421 gatggtggcg accgtcatct cgcggggcgc gagaaaatcg cggccatagc cgatggattg
      481 gccgcgctgt acctgcttca cgaaggcgac ccggctctta agttgccacg ccgggcgcag
      541 ctccagaatc cggggcacgt gcgccgaggg atactggccg tagaggatcg tgccggggcg
      601 caccatatcg agatgggatg cctcgaggcg gaggatggcg gcgctgttcg cggcatggat
      661 caggggccgg cccagcccgg ccttctccac ctccgcgatg gcgcgcagga agagcgagag
      721 ttgcttgcgg acgaaggtga gctcgcgctc caatgccgtg gcgaagtggg tgtagatccc
      781 ctgcacttcg agattgggaa gcgcgtggag cgcgcgcacg aactccaaca gctccgcgca
      841 gcggatgccg atgcgcccca tgccggagtc gaccttcacg tgaacctgcg ccgtagcccc
      901 agaggcgcgt gccgccgccg agagcgcccg ggccgattcc agcgtgcaga ccgtctgcga
      961 gagccggtgc gcgacgacca cgcgcgcctg gttcgggagt gcggcaccga aaaccaggat
     1021 cggcgcgtcg ataccggcgt ggcggagctc tagcgcctcc tccacgcgcg taaccccgag
     1081 gaagtcggct ccggcatcca ggaaggtgcg tgaggcctcc acggcgccgt ggccataggc
     1141 atcggccttg accacggcca ggaggcgcac atcaggaccg atatgggcgc gcacctgcgc
     1201 caggttgtgc gccagcgcgt cgcggcgcac ctcgacccac gccgtatgct cctgcgcggt
     1261 tgccagttct tccatcggta ccttcccgga tgcggggaac ccggcatgat gttatgcggc
     1321 tgcaccacgc cgggcgtgcc tcgcctactt cttcttcgcc ggggtcttct tcttctgttg
     1381 cgcggcccgc ttcttgatca gctcggcgcg cttcttatcc acgcttgcgg gctttccctt
     1441 gcggctggcc agctgggccc tctgacgctt ggcgatcgcc tcggcgacct tgtcatcccc
     1501 gccactcccg ctgcgcttgg ctacagccgt gcgataggcg gtgttcgcgc tcggctccag
     1561 ccattccgcg atgacgcgcg acggagtggt cagctcgtat ccggccatgt aacgcgccgc
     1621 tgcaacaatc gccgcgtagt gaaccacagt ccgatgttcg agggccaggc cccaggctcg
     1681 ccgggtctcc accaggatcg cgggcacgcc ctccttcacc gcgaaatagc ggtgggcgag
     1741 acggggctcg cgatagttga agacggggcg cgcgtagatg cgaatcgact gctcgcgcag
     1801 cggtccgatc accgcggcaa ttgcggcgtt gcaggcagcg cccacgccac ctcgggcgcc
     1861 tgcctccggc tccatcgcct cgacgaagct cggcgtctgg tccgtgggat ggagctcatg
     1921 gagatccatg agcaggtccg gccgccactt gcgcacggcc tgcacgatgg cgcgcgtctc
     1981 cggctgagac tgcgagagcc agtcgcggtt gagatccacg ttgcgcgcgt tacgccgctg
     2041 gtccttctcg acgccatcca cgttgagcat cggcacgacc aggaaggtga cccggcgcag
     2101 gatctcgagc ttcgctggat cttccgtggt ggcatactcc ttcaggaacc gcatcacgcc
     2161 ttcggtgctg gcgggctcat tcccatgctg ccggcacagg atgaatacgc gccgggggcg
     2221 gtcctccccc gctggcggcg ccgctatggc tgcctcgacg gcggcaggtg cttcatccac
     2281 cggatccagc gtcagcgtga gcacggcggg ctcggtcgcg tcgctctcgg gagcgtccac
     2341 gcccggctcc ggtgccgttg ctgcgggagc ctctgcaggc tcctccgccg gctcctctac
     2401 atcgtcggaa ctcttggggt cttccacggg cgcggcgctc aggaccgcca tcagaatcgg
     2461 ctttcctttg agcgtcgtgc cgagctcgac aacgcgcatc tgggggccgc ccggcgaaga
     2521 ggccgacgcg cacagctcct cgagcgagga gaccacatct gaatatgaat agagtgcatg
     2581 agcgtgcgaa acgctcagca acagcattag cagcgggact gcaatacgga tgccggcctt
     2641 gatcaaacct ccgctccttt ccaacacccc tcgccatcca cttgagctac gggccgattg
     2701 tgccagcatc gccccgtctt gcccggagag caaacacgga ccaagccgcg ataccacccg
     2761 tccgctatcg aagtgaacct ccagaccctt ccgatgcgcc caaacgaatg cccgccacgg
     2821 gggacttcac cggcctcgga ggctgttccc gggtatttcg ggctctgtat ggcatccacg
     2881 agcgcctctc catcgggctc ccggagaggg ctggcatcca tgcgatggag gcatgcaatg
     2941 ccgggcgggg atgccagcgg atgtctcgct tcatctgaag ggtatggggt tcacaccccg
     3001 atgctcttgt attggacacg cacccgccat tttccttctc cgagcgctga cgggaacttc
     3061 agaggaggag ttggcagagt gccggcgaag agcgatccgg tgcgcgatca gaagcgcgat
     3121 tcgtcgatag agcggggagg gcgtgttgag agaggagttg gaaacttatc gcgaggcggt
     3181 gcgccgcgat cccgaggatg gcccggcgcg tttgaggttg gcgctcgcgc ttctggatca
     3241 aggtgggccg gatgctgaga gcagtgaaga gctcctcgcc gagctgcgcg aggccgtgcg
     3301 cctctgccca gacaacccgc tggcgcatac catgctgggg gtcgtcctgc gcggaaaagg
     3361 gcttcgcgag gaggccgtct ccgcctggga acaggccgcc cggcttgccc cggaggatcc
     3421 ggtcgtccat gctttcctgg gggacgcgct gtgcgaagcc gggcgttacg cggatgcggt
     3481 gccgcattgg cgccaggcgg tgcgcgcatg ccccgagaac gcggagtatc gagacaacct
     3541 ggtgatcgcg ctcgcgcgtg acattgaaac cacggcgggg gatgccgcag agcggcgcgc
     3601 tcgggcgcag gagcgcctgg cggcggagcc cgacgcaccg gagatgcatg ccgtccttgg
     3661 ttggctgttg agccaggaac gccagctcac cgaggcgatc caccactggc gcgaagcgat
     3721 ccgtctgggg ggcggcaccg ccggcatcca taacaacctg ggtctggcac ttttctatac
     3781 cggggctatc gccgagagca tcgatgagtt tcgcgaggca gcgcgcctcg agccttcact
     3841 ggcggcgtca catacgaacc ggggcacggc gctgcgcgcc cttggccgga gccgggaagc
     3901 ggtggatgcg ttccgcgagg cgctcgccct cgatccggag gagacgacgg cacgccacta
     3961 tctggcggat ctccttgccg agctcaatga accggacgag gcgatctcgc actaccgcga
     4021 ggtgattcgc cgaatgccgc agcgcgcgcc tgcctaccgg ggcctgggcg acgccctcga
     4081 tcagcttggc agggtggagg acgccatggc cgcctatcgc caggcgcttg ccctcgatcg
     4141 tgacgaccct gttgcccggt atcacctggc cgtggcgctc gaccgcaccg gccggcagga
     4201 agaggcgatc cccgaatacc gcgagacgat tcgcctgtgc cccaaccacg cgggcgctca
     4261 caacaacttg ggcgtgctcc tcttcgagcg agcgaaggat ctgccggctg gcccggagcg
     4321 agatgccggg ctgaaggccg ccgatgagca ctattgcgcg gctctccgcg cgaatttggg
     4381 cgacgcggag gctctcttca acctggggga ggtcctctcg ctgacagggc ggccggcgga
     4441 ggccgtggtc gctttccgac aggcgtcccg cctcaacccg gatgatgccg agacgcaggc
     4501 cggactggcc gccgccctgg aggcaactgg cgctcgtgaa gaagccgcgg atgcctggtc
     4561 gcgcgtgctg gcctcgggcg atgccgtttt ggccgcgcac gcggagg
//
LOCUS       DAOSCV010000274         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11865_length_4594_cov_3.363076, whole genome shotgun sequence.
ACCESSION   DAOSCV010000274 DAOSCV010000000
VERSION     DAOSCV010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11865_length_4594_cov_3.363076"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PLY56_08000"
     CDS             <1..355
                     /locus_tag="PLY56_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ21463.1"
                     /translation="QPGTYCTLTREWRDGDTVSFTLPLGIRVTRYTGLDQVPGRNRYA
                     LEYGPVLLGIVGPFNWRGRFTSLKVDPEKPEEWLEPVPGQPGHFTVKGYPRHRVVPYY
                     EIAEERFTCFPALGA"
     gene            979..2448
                     /locus_tag="PLY56_08005"
     CDS             979..2448
                     /locus_tag="PLY56_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOJ21464.1"
                     /translation="MTVEYILVGVGGLLLLSVVASKGSSILGVPALLIFLMIGMLAGC
                     EGPGGIAFDDARLSQGLGVVALIFILFAGGLSTEWRRVHTVLWPSFLLATLGVLITAV
                     LLGGFAVAFLGLTPIEAFTLGAIVSSTDAAAVFAVMRSRNVHLKEHLETLLEFESGSN
                     DPMAVLLTVSMIRLLTAPDTSPLGLVPFFVQQMILGSLLGYLSGRAIVWIINRINLEY
                     DGLYPVLSTTLILLVYALTSLTGGSGILAVYIAGLVMGNSTFLHKNSLTSFHDGLAWL
                     MQIVMFLTLGLLVYPSRLLPLAGIALGASLFLMFVARPVAVFLSLIPFKFPRSEKMMI
                     SWVGLRGAVPIILATYPLLANFPNADAMFHVVFFVVFTSVLIQGPSLPWLARRLRVDR
                     PPAQIARELPSTPAAEGISGSLVEIAVPPGSPFAGRPIVELGLPRGVYIVLVRRGEEM
                     VVPAGSTLINAGDRLLMMGKTEALDSIRSVLAPVYDVAE"
     gene            2645..>4594
                     /locus_tag="PLY56_08010"
     CDS             2645..>4594
                     /locus_tag="PLY56_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ21465.1"
                     /translation="MPITFQHWPWLLLLPPLAAWLVGVHRRSLAGLDPWRWRVALGVR
                     LAGTMLLILALAGTTLVHWSDAQAVWFLVDHSDSMGPEHRRHALDQVTAAAKKMRPGD
                     QAGVILFGADALIEAPLADALSLNQTASTPVTTYTDLARALRLALAAFPDRYARRIVL
                     LSDGNENLGNALEEVALAAAQGVPVDVVPIEQTRGAEALLERMVLPNEVKIGEPFELR
                     VVASSQTAQEATLRLFRNHAYIGAQQVSLSPGKNVVVFVQNLEKPGFHTYEARLEPRD
                     DTLSENNRALGFSLVRGRPRVLYVEGDPGQEKYLAAALRSQQIDADVVNTTGIPRNLA
                     EFQSYDSIVFSNVSALHVSAEQMKMIQSNVRDLGQGFVMIGGDESFGAGGYYRTPIEE
                     LLPVDMDIIKPQEWPSTAVVCVIDKSGSMADGGMGFSKVDLAKEAAIATVEVLDEHDD
                     FGVIAFDQAAKEVVSLQRVRNQKAIQDRIASIVADGGTSLYPALMMAHEWLKKNNARV
                     KHCIVLTDGMSLPGDFKHAVQALNAAGVTLSTVAVGQDADLQLLEGLARGGGGRFYYT
                     DDPWMLPRIFTKEAFLVAKSLIVEEPFVPRVDSSAEVLRGIDWSSRPPLLGYVATSPK
                     ALAAVPMRSPRGDPLFAYWRYGLGRT"
BASE COUNT          724 a         1466 c         1457 g          947 t
ORIGIN      
        1 ccagccgggc acctactgca cgctgacgcg cgaatggcgc gacggcgata ccgtctcttt
       61 caccctgccg ctggggattc gcgtcacccg ctacaccggg ctcgaccagg tcccgggacg
      121 gaaccgctac gccctggagt atgggccggt gctgctgggc atcgtgggtc cgttcaactg
      181 gaggggccgg ttcacctctc tgaaggtgga tcctgagaag ccagaggagt ggctggagcc
      241 ggtgccgggg cagccgggcc acttcaccgt gaaggggtac ccgcggcaca gggtcgtgcc
      301 ctactacgag atcgcggaag agcgcttcac ctgtttcccc gccctgggag catgaggaga
      361 gccagggcac gcatcacgcg cggatcccga agcgagcgtc tgcacccgga gcgccaccgc
      421 aacctgtgcg tgcttttctg gcgctgaagc gcctatttct gcgagctgga cgatcactac
      481 cggggcagat catcgcccga cagaggcatg cgctccaggg gacaggtggt gctatgccgg
      541 cccacgtgga atgcggccga gggaggctct atctgccgca gaccgcccca ccgccctctt
      601 ggccttttcc ccctccttaa taactggtat aatagtgtgg tgcattcagg cactccctcc
      661 ttgggagaaa cggctgggtt gctgatgcgg ccgtaccttc tcaaattggg tatacctggt
      721 tgtaagagag aaatgtctct cgcgtagaac actccgacgt cgcggtaagt ttttttttgc
      781 agagctatcc gtttttggcg gcggggatcc gccactgttc cgcggggcac gccatctggc
      841 ccggcgatcc gcgggccagc gactccctct aggcaacgct cactattgaa gggaccgttt
      901 tgtctcgttc tcagagtctg cgtgcgaggt tgtactgctg attgcgtgat tagccaagaa
      961 ggggaataga caatcgttat gacggtagag tacatcctgg ttggcgtggg gggcctcctt
     1021 ctcctgagcg ttgttgcgag caagggatcg agcatactcg gcgtgcctgc cctcttgatc
     1081 ttcttgatga ttggcatgct cgcggggtgt gaggggcccg gggggatcgc ctttgatgat
     1141 gcccgtctct ctcaggggct gggcgtcgtg gcattgatct tcatcctctt cgcaggtgga
     1201 ttgagcaccg agtggaggcg tgtgcacacg gtgttgtggc cgtcatttct gcttgccacg
     1261 ctcggagtcc tgattaccgc ggtgctcttg ggtgggttcg cggtcgcgtt cctcggcctg
     1321 acccccatcg aggcgttcac actcggtgcg attgtctcct cgacggacgc cgcggccgtc
     1381 ttcgccgtga tgcgctcgcg caacgtccat ctgaaggagc acttggagac gttgctcgag
     1441 ttcgagtccg gcagcaacga ccccatggcg gtgctcctca cggtgagcat gattcgcctg
     1501 ctcacggctc ccgatacttc accgctcggc ctcgttccct tcttcgtcca acagatgatt
     1561 ctggggtcgc tgctgggcta tctctccggc cgggctatcg tttggatcat caaccggatc
     1621 aatctggagt acgatggcct ctatccggtt ctcagcacca ctctgatcct cttggtgtat
     1681 gcgctcacat cgctgaccgg gggaagcgga atccttgctg tctacattgc tggcctggtc
     1741 atgggcaaca gcaccttcct gcacaagaac agtctgacga gcttccacga tggcctggcg
     1801 tggctgatgc agatcgtcat gttcctcacg ctgggactgc ttgtctaccc gtcgcgtctc
     1861 ctccccctgg cggggatcgc gctgggcgct tcgctctttc tcatgttcgt tgcccggccg
     1921 gtcgctgtct tcctgagcct gatccctttc aagttcccgc gcagcgagaa gatgatgatc
     1981 tcatgggtgg gattgcgtgg cgcagtgccg attatcctgg cgacctaccc cctgctggcc
     2041 aacttcccga acgccgatgc gatgttccac gtcgtcttct tcgtggtgtt tacctcggtc
     2101 ctgattcagg ggccctcgct gccttggctc gcccgcaggc ttcgggtgga taggccgccg
     2161 gcgcagattg cccgcgagct gccatccacg cctgctgccg aaggaatctc cggatccctg
     2221 gtggagattg cggtgccgcc cggttccccc ttcgcggggc gacccattgt tgaactggga
     2281 cttccacgcg gtgtctacat cgtcctggtg cgcagaggcg aggagatggt ggtgccagcc
     2341 ggctccaccc tgatcaacgc cggcgatagg ctgctcatga tgggcaagac cgaggccctg
     2401 gacagcatcc gttccgtcct cgctcccgtg tacgacgtgg ccgagtagct ggcgcgcggg
     2461 cgagcaccag ccatccgtcc ctttcacccg tggtatcggc gccggcgtgg ccggggggca
     2521 gagcgatggc tcgcttttcc tcgcggctgc accgtggtat aatggggtgt cggccgtgct
     2581 caccattggg cggctctcca gacacccgtc gccgcggcgg ccggcgcgcc gggagcgatg
     2641 cgtcgtgccg atcacatttc agcattggcc ctggttactg ttgctgcccc ctctggctgc
     2701 ctggctggtg ggcgtacacc gccggtcact cgccggcctg gacccctggc gctggcgggt
     2761 tgccctgggc gtgcgcctgg ccggcaccat gcttctcatc ctcgcccttg ccggcaccac
     2821 cctcgtccac tggagcgacg ctcaggccgt ctggttcctt gtggatcact ccgatagcat
     2881 ggggcccgag caccgccgac atgctctcga ccaggtaact gccgccgcca agaagatgcg
     2941 tcccggagac caggcgggcg ttattctctt tggcgcggat gccctcatcg aagccccgct
     3001 ggctgatgcg ctctccctga atcagacggc gagcacgcct gtcaccacct atacggacct
     3061 tgcgcgcgcc ctgcgcctgg ccctcgccgc gttcccggac cgatatgccc ggcgcatcgt
     3121 gctcctctct gatgggaatg agaaccttgg caacgccctt gaggaggtgg cgctcgccgc
     3181 ggcacagggc gtgccggtcg atgtagtgcc catcgagcag acacggggcg ccgaggcgct
     3241 gttggagcgg atggtgctcc caaacgaggt gaagatcggc gagccgtttg agctgcgcgt
     3301 ggtggcctcc tctcagacag cccaggaggc aaccctgcgc ctcttccgca accatgccta
     3361 catcggggcg cagcaggtga gcctctcgcc gggcaaaaac gttgttgtct ttgtccagaa
     3421 cctcgagaaa ccaggctttc atacctatga ggcgcgcctg gaaccacgcg acgatacgct
     3481 ctccgagaac aaccgggcgc tcggcttctc gctggtgcgt ggccggccgc gcgtgctcta
     3541 tgtcgagggc gatcccggac aagagaagta cctcgcagcg gcccttcgct cgcagcagat
     3601 cgatgccgac gtcgtcaaca cgaccggaat cccgcgcaac ctggcggagt ttcagagcta
     3661 cgatagtatt gttttcagca acgtctccgc gctccacgtc tctgccgagc agatgaagat
     3721 gatccagagc aacgtgcgcg acctgggcca aggcttcgtc atgattggcg gcgacgagag
     3781 ctttggcgcg ggtggctact accgcacgcc aatcgaggag ctcctccccg tcgacatgga
     3841 catcatcaag ccccaggagt ggccgagcac tgcggtcgtc tgcgtcatcg acaaatccgg
     3901 gagcatggca gacgggggga tgggcttttc caaagtggat ctcgccaagg aggccgcgat
     3961 cgccaccgtc gaggtgctgg acgagcacga tgattttggg gtgatcgcct tcgaccaggc
     4021 ggccaaagag gtggtctcgc tgcaacgagt gcgaaatcag aaggcgattc aggaccggat
     4081 tgcgtcgatt gtggccgacg ggggcaccag cctctacccg gcgttgatga tggcccacga
     4141 gtggcttaag aaaaacaacg cccgtgtgaa gcactgcatc gttcttacag acggcatgtc
     4201 gctcccgggc gacttcaagc atgccgtcca ggcgctgaac gccgctggcg tcacgctctc
     4261 caccgtcgct gtcggccagg acgcggatct ccagctgctc gagggacttg ccaggggtgg
     4321 cggcgggcgc ttttactaca ccgatgaccc gtggatgctc ccgcggatct tcaccaagga
     4381 ggcgttcctc gtcgccaagt cgctcatcgt cgaggaacca ttcgttccga gggtggactc
     4441 cagcgcggag gtgctgcgcg gcatcgattg gtccagccgg ccgccgctgc tcggctacgt
     4501 cgccacttcg ccgaaggcgc tggctgcggt gccgatgcgg agcccgcgcg gcgacccgct
     4561 ctttgcctat tggcgctatg gcctgggccg tacc
//
LOCUS       DAOSCV010000275         4588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11884_length_4588_cov_5.774542, whole genome shotgun sequence.
ACCESSION   DAOSCV010000275 DAOSCV010000000
VERSION     DAOSCV010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11884_length_4588_cov_5.774542"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="PLY56_08015"
     CDS             complement(<1..671)
                     /locus_tag="PLY56_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ21466.1"
                     /translation="MPTITAHLRDLQTLIGREISLSELEERMPLAKAELKRDEDGELT
                     IEFNDTNRPDLWSAEGLARQIRAYEEGKSRSYTCFVSPKEAPSVAASSEAIIHVSPAL
                     QEVRPYIAAFLVKGITIDDEGLRQLIQSQEKLSENFGNKRAAVSVGTYRAAHITWPVR
                     YADTDPGSTSFVPLGFDEPMTLREILERHPKGIDYGYTLEGKARYPLLIDAKDEILSF
                     PPIIN"
     gene            complement(747..2312)
                     /locus_tag="PLY56_08020"
     CDS             complement(747..2312)
                     /locus_tag="PLY56_08020"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF003210.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ21467.1"
                     /translation="MADSDAGRNALLESLHALEVRALQVLGSREQVDEEELAEAAGIQ
                     EAQARTALGWLLSKELVYVVSEETRREVRLTETGESYAERGTPEERIWRHLRSAGPTA
                     MPALIAAAGIEKEEQSGAIGMLRATGAITIGPGGIVSAPDTAEPEVVARGRDLLRAVQ
                     ERQPAPLESFTEEEQQRLQEMAGGKRKGKGLVSIDVRRHREYALTEEGKALVQLLAER
                     GYREEVSQLTPAMLQDGSWRQVRFRPYYIGLNPPRALAGKFNSYGEFLQFVRSKLVAM
                     GFTEVRGSLVENEFWDMDALYMPQFHPARNVHDVYFVKEPEYSEPVLEPFATRVAQAH
                     ENGGDTGSRGWEYRFDLQRSRRLILRSQGTALSARTLAAGACIPGKYFAVARCFRYDQ
                     VDATHASDFFQVEGIVVSESVNFRTLLGLLKLFAVEIARATEVVFAPAYFPFTEPSVE
                     MHAKHPTLGWMELGGAGLFRPEVTRPLGVSEPVIAWGLGVDRMAMLALGLHDIRDLFS
                     ADLDLVRTRKFNP"
     gene            complement(2914..>4588)
                     /locus_tag="PLY56_08025"
     CDS             complement(2914..>4588)
                     /locus_tag="PLY56_08025"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ21468.1"
                     /translation="REVPRLGVNFRVVAEEDEIQVVAPVMDADTSLGAILINISLREH
                     HALLDAVRQWGVLMALTTILIACTTAYFLAAVITRPLQRLAHGMRAVAEGNPAPPLDE
                     DGCREVRQMAKSFNGMARSLRRRLAEMESLRILGSATSRSLDLGTVIQSASECVEAAI
                     PDIRAQIWLCDREGGRLIEGGRAAVEPEQVIDLDSDTVLARALQSPEPLLAGAGSEVQ
                     LDPWLAERYQATSVAAVPLRAGQERVGVLLACRQNGTPLTRDNLPFLEAAGTEIALSI
                     QNARMFHRESRVAEVFQRMLLPQTHTFIEQLEVAVRWRPASGDGQIGGDYYDFIALPG
                     GRWGIVIGDVCGKGTLAASYTAMAKYALRSYAYETGSPAEILRRTNAALYAQLNSTEA
                     HEGAPSFITMLCALYDPSTRQLVYSHAGHPLGTLACANSHVVITLDQGGPPVGVVPDA
                     TYQEDVLYLAHGDSLALYTDGVVEASRSEEVIEPARIAELLRMNQENSVHAAADAVLA
                     EAMNAAGGHAADDTTLLVIRVRDLLPPLALPAPSAAETPLPGSVECQPC"
BASE COUNT          763 a         1546 c         1481 g          798 t
ORIGIN      
        1 ctgttgatga tcggcgggaa ggagaggatc tcatccttgg catcgatcag cagcgggtag
       61 cgggccttcc cctccagggt atagccatag tcgatccctt tgggatggcg ctccaggatc
      121 tcgcgcagcg tcatcggctc gtcgaagcca agcggcacga acgaggtgct cccgggatcg
      181 gtgtcggcat agcgcaccgg ccaggtgatg tgcgcggcgc ggtacgtccc cacggagacg
      241 gcggcacgct tgttcccaaa gttctccgag agcttctcct ggctctggat gagctggcgt
      301 agcccctcgt catcgatggt gatccccttc accaggaagg ccgcgatgta aggacgcacc
      361 tcctgcaatg cggggctcac atggatgatc gcctcgctgc tcgccgcaac cgagggggcc
      421 tccttgggag agacaaagca agtgtaggag cgcgacttcc cctcctcata ggcgcggatc
      481 tgccgggcca acccctcggc cgaccagaga tcgggccggt tggtgtcgtt gaactcaatg
      541 gtcagctcgc catcctcgtc gcgcttcaat tccgccttgg ccagcggcat tcgctcttcc
      601 agctccgaga gcgatatctc cctgccgatg agggtctgca aatcacgcaa gtgtgctgtt
      661 attgtcggca tctattctgt tccaatcaca cactaacccc gcaggttcca tgctggaacc
      721 ccacaagctc tcgccgtggg aggacgtcag gggttgaact tccgcgtgcg caccaggtcg
      781 agatccgccg agaagaggtc gcgaatatcg tgcagaccca gggccagcat ggccatgcga
      841 tcgacgccca ggccccaagc gatcaccggt tcgctcactc ccagcggccg cgtcacctca
      901 gggcggaaga ggccggcgcc gcccagctcc atccatccga gcgtcggatg cttcgcgtgc
      961 atctctaccg aaggctcggt gaaggggaag taggccggcg cgaagaccac ctcggtggcg
     1021 cgggcaatct ccaccgcgaa cagcttcagc aggccgagga gcgtgcggaa gttcacgctc
     1081 tcgctcacca cgattccctc gacctggaag aagtcgctgg cgtgggtggc atccacctgg
     1141 tcgtagcgga agcagcgcgc gacggcgaag tacttgcccg gaatgcacgc gccggccgcg
     1201 agcgtgcgcg cggaaagcgc cgtgccctgc gagcgcaaga tgaggcggcg gcttcgctgc
     1261 aagtcaaacc gatactccca gccgcgcgag ccggtatcgc cgccattctc atgcgcctgg
     1321 gccacacgag tggcgaacgg ctctagcacg ggttcggagt attccggctc cttcacgaag
     1381 tagacgtcgt gcacgttgcg cgcgggatgg aactgcggca tgtaaagcgc gtccatgtcc
     1441 cagaactcgt tttcgaccag ggacccgcgc acctcggtga agcccatcgc cacaagcttc
     1501 gagcgcacga attggagaaa ctcgccgtag gagttgaact tgcccgcgag ggcgcgcggc
     1561 gggttcagtc cgatgtagta gggccggaag cgcacctgac gccatgagcc atcctggagc
     1621 atcgcggggg tgagctgcga gacctcctcg cgatagccgc gctcggcgag cagctggacg
     1681 agcgccttcc cttcctcggt gagcgcgtac tcgcggtgcc ggcgcacgtc aatcgaaacc
     1741 aggcccttcc ccttgcgctt tccgccggcc atctcctgga ggcgctgttg ctcctcctcg
     1801 gtaaagctct cgagcggcgc gggctggcgc tcctgcacgg cgcgcagcag gtctcgccct
     1861 cgggcgacca cctccggctc ggccgtgtcc ggcgccgaga caatgccgcc cgggccgatc
     1921 gtgatcgcgc ccgtggcgcg cagcattccg atggcgccgc tctgctcttc cttctcgatt
     1981 ccggcggccg cgatcagcgc cggcatcgcc gtggggcctg ccgagcgcag atggcgccag
     2041 atccgctctt ccggcgttcc acgctccgca tagctctccc ccgtctccgt gaggcgcacc
     2101 tcgcgccgcg tctcttcgga gacgacatag accaactcct tgctcagcag ccaacccagc
     2161 gcggtgcgtg cttgcgcttc ctgaatcccg gcggcttccg cgagctcttc ctcgtccacc
     2221 tgctcgcgcg agcccagcac ctgcagtgct cgcacctcca gcgcatggag actctcgagc
     2281 aacgcgttcc gcccggcatc actatctgcc atggttggtg gtcactcctc ttccacttca
     2341 aagccaagaa ccagtactca ttatctattc gcctccaaga ccaccaatcc tgcctccgat
     2401 gaaaaccgca cgcttgtccc gcagccaacc gcgcgctccc gaacggggga cgggcacccc
     2461 gtccgtgtcc gtcaccatca ttcgcggtat ggaagcctgg gattccgaca taaggagagg
     2521 gccgcgcgca cgcgcggagc tcgaagctcc gcgctgcttt ccggcaaagc ccttcggggc
     2581 ttctcctccc agccaccgcc gttttgagac atctgggagt atccccaacc cagcccggca
     2641 gggcttcgct cgcaagcagc gcggtgattt atcgccgcgc gccgggttgc gtgctgcctc
     2701 cgtagcttcg gtttccttat atcggaatcc cagtttccca gactggattc cgggataagg
     2761 aatccgggcc cggggacaga aactcccggc acgaatgccg gacctctacc atatcctcgc
     2821 ggttagccct gcctggcacc gaaggggccg tgcgacttag cccagggtgt ccgcaccctg
     2881 ggtgtgcacc ggcgcccggg aaaccggagt ctttcagcac ggctggcact cgacactgcc
     2941 cggcaggggc gtttcggctg cggatggggc aggcagagcc aggggcggaa gcaggtcgcg
     3001 gacgcggatc accaggaggg tggtgtcgtc ggcagcgtgc cccccggccg cgttcatcgc
     3061 ctccgcgagc acggcgtctg cggcggcgtg gacggagttc tcctggttca tgcggaggag
     3121 ttcggcgatg cgcgcgggct cgatgacctc ctcgctgcgt gacgcctcca ccacaccatc
     3181 ggtgtagagc gccagggaat cgccgtgtgc caggtaaagc acgtcttcct gataggtggc
     3241 atccggtact acgcctaccg gaggcccgcc ctggtcgagg gtgattacga cgtgcgagtt
     3301 cgcgcacgcc agcgtgccca ggggatggcc ggcgtgagaa tagaccagct gacgggtgga
     3361 tgggtcgtag agggcgcaga gcatggtgat gaagctggga gcgccttcgt gcgcctccgt
     3421 gctgttgagc tgtgcgtaga gcgcggcgtt ggtcctccgc aggatctccg cgggcgaccc
     3481 agtctcgtag gcataggagc ggagggcata tttcgccatc gccgtataag aggcagcgag
     3541 cgtgcccttc ccgcagacgt ctccgattac gattccccat cgcccgcccg ggagggcgat
     3601 gaagtcgtag taatctcccc caatctggcc gtcccccgaa gcggggcgcc accggacggc
     3661 gacctccagt tgttcgatga aggtgtgcgt ttgcgggagc aacatccgct ggaacacctc
     3721 ggccacgcgc gattcgcggt ggaacatccg cgcgttctgt atggagaggg cgatctcggt
     3781 gccggcggct tcgaggaagg gcagattgtc gcgcgtgagc ggggtgccgt tctgacggca
     3841 ggccagcaag acgcccacgc gctcctggcc ggcacggagc ggcacggcgg ccacgctggt
     3901 tgcctggtag cgctctgcca gccaggggtc gagttgcacc tcggagccgg cgcccgccag
     3961 gagcggctca gggctctgca gcgcgcgtgc aagcaccgta tcggaatcga gatcgatcac
     4021 ctgctctggc tccacggctg cccggccccc ctcgatcagg cggcccccct cgcggtcgca
     4081 gagccagatc tgggcgcgaa tgtcgggaat ggcggcctcg acgcactcgc tggccgattg
     4141 gatcaccgtg cccaggtcga gcgagcgcga ggtagctgag ccgaggatac gcaggctctc
     4201 catctctgcc aagcggcgtc ttagcgaccg cgccattccg ttgaagctct tggccatctg
     4261 ccgcacttcg cgacagccgt cctcgtccag gggcggcgca gggttgcctt ccgctacggc
     4321 gcgcatcccg tgggccaggc gttgcagcgg ccgcgtgatc actgccgcga gaaagtaggc
     4381 ggtcgtgcag gcaatcagga tcgtcgtcaa cgccatcagc accccccact ggcgcaccgc
     4441 gtcgaggagg gcgtggtgct ctcgcaagga gatgttgatc aggatggcgc caagggatgt
     4501 gtctgcgtcc atcaccggcg cgaccacctg aatctcatcc tcctccgcga cgacccggaa
     4561 gttgacgcca aggcggggta cctcgcgc
//
LOCUS       DAOSCV010000276         4586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11890_length_4586_cov_2.742000, whole genome shotgun sequence.
ACCESSION   DAOSCV010000276 DAOSCV010000000
VERSION     DAOSCV010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_4586_cov_2.742000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLY56_08030"
     CDS             complement(<1..1177)
                     /locus_tag="PLY56_08030"
                     /inference="COORDINATES: protein motif:HMM:NF016858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ21469.1"
                     /translation="MANGANFDKIRIGIASPEEIRSWSSGEVKKPETINYRTFKPERD
                     GLFCERIFGPVKDWECHCGRYKKIKFKGIICDRCGVEVTRAKVRRERMGHIELAAPVS
                     HTWYLKGVPSPMSLILDVAPRPLEKVLYFVSYIVTHMDRAFLQDHWDAIKEAVSDQIR
                     EEEAARDEHIAALKAQLEEELADEELTDEERAQKRSLQLDRERLEQRNAEDKAKELTE
                     GLQLLQDKDEKQLITEAEYRVMRRVLDVATERTGIDFTKAFRAGMGAAAIKELLEKIN
                     LEELSRQLRKETEESQGAKKLRAIKRLEVVRAFLRSKSRPEWMILEVVPVIPPELRPI
                     VQLDGGRFATSDLNDLYRRIINRNNRLKKITQIRAPESIINHEKRLLQEAVDALIDNG
                     "
     gene            complement(1589..2473)
                     /locus_tag="PLY56_08035"
     CDS             complement(1589..2473)
                     /locus_tag="PLY56_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ21470.1"
                     /translation="MADLTVYFGDNLEVLRSLPTGTVDLIYIDPPFNTGKVQCRTSHA
                     MIRDSNGDRTGFQGMRYRTVKLGSREYSDIFDDYLAFIEPRLREAHRVLKNNGSLFFH
                     IDYREVHYCKVLLDLVFGRESFKNEIIWAYDYGARPKKSWPAKHDNILWYAKDPENYQ
                     FHLEECDRIPYMAPGLVGPEKAARGKTPTDTWWHTIVSPTGHEKTGYPTQKPRGIVDR
                     IVKVHSRPGDLLLDFFAGSGTLGESCVALGRRCILVDNNPEALRVMERRFAQTAVDWR
                     NWTPEVQSGAAMQGSLCT"
     gene            complement(2564..>4586)
                     /locus_tag="PLY56_08040"
     CDS             complement(2564..>4586)
                     /locus_tag="PLY56_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ21471.1"
                     /translation="DANRALMGSNMQRQAVPLLKSERPIVRTGVEFRAAVDSGVVVRA
                     RNDGIVERVTAREIAIPRRDGELDEYRLLHMLRSNQATCITQRPIVKAGDRVRKGQVI
                     ADGPSTDHGHLALGKNVLVAFMPWEGYNYEDAILISERLVMDDVYTSIHIEKYELEAR
                     DTKLGSEEITRDIPNVGEDALRNLDEFGIVRVGAEVTAEDILVGKVAPKGQGELTAEE
                     RLVIAIFGKKAEETKDASLRLPHGEKGRVVDVKVYARFKYRCQQCGSELLLSKKLEDR
                     EAVCRQCGNPMVREAGDELSAGVNQLVQVYVAQKRPIMEGDKMAGRHGNKGVISKILP
                     VEDMPYLADGTPVDIVLNPLGVPSRMNIGQILETHLGMVGRLLGLEEGFVTPIFQGKP
                     EDQILLNVQRLAYHLRNRVLHQYVTMDLGAAGIEGFEGIALPEPTVSSADIDQLPSIE
                     EREAAYAKREQEIYEANLAHVEQSLAKLTEEQQNRLSETLAVLPEKWEAAKSAQDRAR
                     LLVEQIGRVVDERVGMDADTAKFVLYDGKTGQRFDQPVTVGYSYLLKLLHLVEDKIHA
                     RSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGSAYTLQEILTIKSDDVMGRVK
                     TYEAIVKGEPILEPGIPESFKILVKELQSLGLQVSVESDKGKPFDLRDM"
BASE COUNT          766 a         1566 c         1312 g          942 t
ORIGIN      
        1 ggccgttatc gatcaaggca tcgacggctt cctggaggag gcgcttctcg tggttgataa
       61 tgctctccgg cgcgcggatc tgggtgatct tcttcaaccg gttgttccgg ttgatgatgc
      121 gccggtagag atcattcagg tccgacgtcg cgaagcggcc accatccagc tgaacgatgg
      181 ggcgcagctc cggcgggatg accggaacga cctccaggat catccactcc ggccggctct
      241 tcgagcgaag gaaggcgcgc acgacttcga gccgcttgat cgcccgcagt ttcttcgcgc
      301 cctggctctc ctcggtctcc ttgcggagtt gccggctcag ctcctccagg ttgatcttct
      361 ccaacagctc cttgatcgcc gcggcgccca tgcccgcgcg gaacgccttg gtaaagtcga
      421 tgcccgtgcg ctccgtcgcc acatcgagca cccggcgcat cacccggtat tccgcctcgg
      481 tgatgagctg cttctcatcc ttatcctgaa ggagttgcag gccctcggtg agctccttgg
      541 ccttatcctc cgcgttgcgc tgctccaggc gctcgcgatc cagctggagg gagcgcttct
      601 gtgcacgctc ctcgtcggtc agctcctcat cagcgagctc ctcctccagc tgcgccttga
      661 gcgctgcgat gtgctcgtcg cgcgccgcct cttcctcgcg gatctggtcg gataccgctt
      721 ccttgatcgc gtcccagtgg tcctgcagga acgcacggtc catatgggtg acgatatagg
      781 agacgaaata gagtaccttt tcgagcgggc gcggcgccac gtcgaggatg aggctcatcg
      841 ggctgggcac gcctttgagg taccacgtat gcgagacggg cgcggcgagt tcaatatgcc
      901 ccattcgctc gcgccggacc ttcgcccgcg ttacttcaac accacatcgg tcgcaaataa
      961 tccccttgaa tttgatcttc ttatagcggc cgcagtgaca ctcccagtcc ttcaccgggc
     1021 cgaaaatccg ctcgcagaaa agcccgtcac gctcaggctt gaacgtgcgg tagtttattg
     1081 tctccggctt cttgacctct ccggaagacc aggaccggat ctcctctggg gaggcgatac
     1141 cgattcttat cttgtcgaag ttcgcgccgt tggccatacg tattccttcc atgagagttg
     1201 ccaatccggg ccccggcggg tcacgtcgga caacccgcaa cccggagcgc acatcgcagg
     1261 gccagtaagg aacagggagt ctgccgctcg ttccggtgcc gcgcccatgc tgtggctaat
     1321 cctcatccaa ttcctcgcga atgctcccga cggcaaatcc cgtcatctcc gccagcagag
     1381 agatagcatc cgaaagcgag cctccataag gcttcagcca ctcctggggg cgccggcgcc
     1441 ttttctccga tcgctcccac ctgccgagag gggcggctat tgatccactc aaacgggctc
     1501 tccccccgcg cctggtcctc gctcgcgtac tccgcgcgca atgcctcgct gatgcccgcc
     1561 agaatgcggg cctcaggatc gcgaatggtc acgtgcacag actcccctgc atcgccgcgc
     1621 cggactgaac ttcgggcgtc cagttcctcc agtcaaccgc cgtctgcgcg aagcgtcttt
     1681 ccatcacccg cagagcttct gggttgttat ccaccaggat gcacctgcgc cccaacgcga
     1741 cacaagactc cccgagcgtc ccgctgccag caaagaaatc cagcagcagg tcgcccgggc
     1801 gcgaatggac tttcacgatc ctgtctacga tccctctggg cttttgggtc gggtagccgg
     1861 tcttctcgtg cccggtgggg ctgacgatcg tgtgccacca cgtgtccgtc ggcgtcttgc
     1921 ccctggccgc cttctccggt cccaccagac ccggtgccat gtagggaatg cggtcgcact
     1981 cctcgagatg gaactgatag ttctccgggt ccttggcata ccagaggatg ttgtcgtgct
     2041 tggcgggcca gcttttcttg ggccgcgcgc cgtaatcata agcccagatg atctcgttct
     2101 tgaacgattc tctcccgaag accagatcga ggagcacttt gcagtaatgc acctcgcgat
     2161 agtcgatgtg gaagaacaag ctgccgttgt tcttcaacac acgatgcgct tcccgcagcc
     2221 gcggctcaat gaaagccaga tagtcatcga agatgtcgct gtactccctc gagccgagct
     2281 tcaccgtcct gtagcgcata ccctggaacc cagttcggtc cccattgctg tcacggatca
     2341 tggcgtgtga ggtacggcac tggaccttcc cggtgttgaa cggtggatcg atgtaaatca
     2401 gatccaccgt tcccgtcggc aagctgcgga gtacttccag attatctccg aagtaaaccg
     2461 tcaggtccgc caccctcaat ccctcacacg cgctcttacc gactaatacc ggcgtagctt
     2521 gcgttgagcc tggcgctccg cctcggcatc cgcgtactcc tcatcacatg tcgcgcaggt
     2581 cgaacggctt ccctttatcg ctctccaccg acacctgcaa ccccaggctc tgcagctcct
     2641 tcaccaggat cttgaacgac tcgggaatcc ccggctccag gatcggctcg cccttcacga
     2701 tcgcctcgta cgtcttcacg cgacccatca cgtcgtccga tttgatcgtc aggatctcct
     2761 gaagggtgta ggccgagcca tacgcttcca gtgcccacac ctccatctcg ccgaagcgct
     2821 ggccaccaaa ctgcgccttt ccacccagcg gttgctgcgt gatcagcgag tatggtccgg
     2881 tggagcgggc atggatctta tcctccacca ggtggagcag cttcagcaga taggagtagc
     2941 ccaccgtcac cggctgatcg aagcgctggc cggtcttccc atcgtagagc acgaacttcg
     3001 cggtatcggc atccatcccc acgcgctcgt ccaccacgcg gccaatctgc tcgaccagca
     3061 ggcgggcgcg gtcttgcgcg ctcttcgccg cttcccactt ctccggcaaa acagccagcg
     3121 tctcagacag ccggttctgc tgctcctcgg tgagcttagc caggctctgc tcgacgtggg
     3181 caaggttcgc ctcatagatc tcttgctcgc gcttcgcgta ggcggcctcg cgctcctcga
     3241 tcgagggcaa ctgatcgata tccgccgacg aaacggttgg ctccgggagc gcgatcccct
     3301 cgaagccctc gatgccggcg gcgcccaggt ccatggtcac gtactggtgc aggacgcgat
     3361 tgcgcaggtg atacgccagc cgctgcacgt tgagcaggat ctgatcctcc ggcttgccct
     3421 ggaagatcgg cgtcacgaag ccctcttcca ggcccaggag acggccgacc attcctaggt
     3481 gcgtctccag gatctggccg atgttcatgc gcgagggcac gccgagcggg ttgagaacga
     3541 tgtcgaccgg cgttccgtcg gccaggtagg gcatatcttc cacgggaagg atcttcgaga
     3601 tgacgccctt gttcccgtgg cgcccggcca tcttatcgcc ctccatgatc gggcgcttct
     3661 gggccacata gacctgcacc agctggttca cgcccgccga cagctcgtcg cctgcctcgc
     3721 ggaccatcgg gttcccgcac tgccggcaca ccgcttcgcg gtcttccagc ttcttcgaga
     3781 gaagcagctc ggagccgcac tgctggcagc ggtacttgaa gcgcgcatac accttcacat
     3841 cgacgacgcg ccccttttcg ccgtgcggca ggcggagcga ggcatccttc gtctcctcgg
     3901 ccttcttgcc gaagatggcg atcaccaggc gctcctcggc ggtgagctct ccctgaccct
     3961 tcggcgccac cttcccaacg aggatatcct ccgcggtgac ctcggcgccc acgcggacga
     4021 ttccgaactc atcgaggttg cgcagcgcgt cctcgcccac gttggggata tcgcgcgtga
     4081 tctcctcgct gccgagcttg gtatcgcgcg cctccagctc gtacttctcg atgtggatcg
     4141 acgtgtacac gtcgtccatc accagacgct cggagatcag gatggcgtcc tcgtagttgt
     4201 agccttccca gggcatgaac gcgaccaaca cgttcttgcc caaggcgagg tggccgtgat
     4261 cggtggacgg gccatcggcg atgacctggc cctttcgcac gcggtcgccc gctttgacga
     4321 tggggcgctg ggtgatgcac gtggcctggt tgctgcgcag catgtgcagc agccggtact
     4381 catctagctc gccgtcgcgg cgggggatag cgatctcgcg cgccgtcacg cgctccacga
     4441 tgccatcgtt gcgcgcgcgc acgaccacac cagagtcgac ggcagcccga aactcgacgc
     4501 ctgtgcgcac gatcggccgc tccgatttga gcagcggcac ggcctggcgc tgcatgttgg
     4561 agcccatcag ggcgcggttg gcgtcg
//
LOCUS       DAOSCV010000277         4583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11895_length_4583_cov_3.285777, whole genome shotgun sequence.
ACCESSION   DAOSCV010000277 DAOSCV010000000
VERSION     DAOSCV010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11895_length_4583_cov_3.285777"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLY56_08045"
     CDS             <1..1032
                     /locus_tag="PLY56_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21472.1"
                     /translation="PWADNPVGFVVVGLGMGHNRAKMVTQTPGTRLVGVCDLIEERAR
                     RTGEECGVPYSTDLRRWLEDDQVEVVWVMTETGRHAEVAIQALEAGKHVLTTKPMEAN
                     VAACDAMIRAAEAKGLLLGVDLDRRHITSVCTLKAAVAQGRLGKLISGNSSLKVLRTM
                     EYFRANGGWRGTKRWDGGGVLSNQAVHHVDEAAYTLGIPARVRCNVYTQTHDIEAEDL
                     GTAVWEYDSGMVLTFYATTSYPHSTWYYQYELTGTDGAYLEVAGGPFEKPLIRWYIDG
                     AWSEQPPLRVEPEWLNSADNFAAALRAGAELTCTGRDGRRTRAILDAMYRSAYECDGG
                     WVDVEPEME"
     gene            1452..>2785
                     /locus_tag="PLY56_08050"
     CDS             1452..>2785
                     /locus_tag="PLY56_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ21473.1"
                     /translation="MSSDHEALKQASERLESLERELRRTREELYRLMALVDRGPATVF
                     LWRIAPRWPVEYVSANISRFGYDPEEFRSGRISWAELIHPDDTPRLDAEITRAIQDGT
                     HGLVLEYRIITRSGEVRWVEEHALGLLDEHHRLTHYQGIILDITARKRAEAALLAGKE
                     ELESRVAERTADLTRANEKLRREIAQRQRAMEALQLDETRLEALLDLNLMTEASVKEI
                     THFAMEEAVRLTRSTLGYLAFVNESETVMTMHAWSERAMYDCLIAEKPLIYPVETTGL
                     WGEAVRQRRPIITNDYQAPNPWKKGYPEGHVELRRHMNVPIMEGGRVVAVIGVANKET
                     EYDDADVRQLRLLGTGMWRILERKRVVDALKENEERYRSLFAVEPDALVLLDAQTGQI
                     LEANDSACQLFGYELEEFLSLTALDISAEPEKTRAVMDGWQPGRTDKVPYRNS"
     assembly_gap    2786..2795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2796..3490
                     /locus_tag="PLY56_08055"
     CDS             <2796..3490
                     /locus_tag="PLY56_08055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21474.1"
                     /translation="LRKDGTIFPTEIAIRHFELQGRRLLYAAYRDITARVRAEAEMEE
                     AHERLLRAEIEKKQFYRQVIRAVTNNKLHLVDLAQIPVEGELVLETSFDDPESYRALR
                     QRVQQAAITAGMPEENAADFMLAVSEAAANAIKHAKDGRCALYVTPERVIARVSDRGT
                     GIHPENLPATILTPGFSTKVSLGMGYTLMLEVADEMWLTTGPTGTVVQLAKWIYPERH
                     VKEPDLQMWNNL"
     gene            complement(3514..>4583)
                     /locus_tag="PLY56_08060"
     CDS             complement(3514..>4583)
                     /locus_tag="PLY56_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21475.1"
                     /translation="RPAGLKVCLTDSPFHIMEALWAREPDFAEAYGETDPPGKRLCLS
                     YRGKYYQKEIERIVERYRRRRPDLISLDVECFGHALEHVQKCAPCRRLLDDRGVTPVE
                     LATDLYAETARELAAAIAQAARDLGQTPPPIGFYHASPAYRYQNVFDFAKLYPAGCQF
                     NNPEVYVRCWPAAAAEIIRADKRLLPPECPIIAWTSPGTLDWEGEAPSGRLFDTLMEE
                     LLNGATGTLYFLPVHLCSEDLAAQAQVARLLAPFEEIIATSTLCDTKEWRQGSTGHVS
                     AIRSGDEMLVLLADYEHLGKTTVEARIPVERPVDAVELFTGKRTRLAPGANLLRVTIE
                     GGYRSRPFYIGNRWRERVARR"
BASE COUNT          849 a         1490 c         1496 g          738 t
ORIGIN      
        1 ccctgggcgg ataatccggt gggcttcgtc gtcgtcggcc ttggcatggg gcacaaccgc
       61 gcgaagatgg tcacccaaac ccccggcacg cgcctggtgg gcgtttgcga cctgatcgaa
      121 gagcgcgccc ggcgcaccgg cgaagagtgc ggcgtgccct attccaccga cctgcgccgc
      181 tggctggaag atgaccaggt ggaggtcgtt tgggtgatga ccgagacggg ccggcacgcc
      241 gaggtagcca tccaggccct ggaggccggc aagcacgttc tcacaacgaa gccgatggaa
      301 gcgaacgtcg cggcgtgcga tgccatgatc cgcgccgcgg aggcaaaggg gctccttctg
      361 ggcgtggatc ttgaccgccg gcacatcacc agcgtctgca ccctgaaggc agccgtggct
      421 cagggccggc tcggaaagct catcagcggc aactcctcgc tgaaggttct gcgcacgatg
      481 gagtacttcc gggcgaacgg cggctggcga ggcaccaaac gctgggatgg cggcggcgtg
      541 ctctcgaatc aggcggtcca ccatgtggat gaggcggcct acaccctggg aatccccgcg
      601 cgcgtgcgct gcaacgtcta cacccagacg catgatatcg aagcggagga cctgggaacg
      661 gcggtgtggg agtacgactc cggcatggtg ctcacgttct acgcgacgac cagctaccca
      721 cactccacct ggtactacca gtatgagctg acgggaaccg acggcgccta tctcgaagtg
      781 gcgggagggc ccttcgagaa gccgctcatc cgctggtaca tcgatggcgc ctggagcgag
      841 caaccgccgc tgcgcgtgga gcccgaatgg ctgaattcgg cggacaactt cgcggccgcc
      901 ttgcgcgctg gcgcggagct cacctgcacc ggacgcgacg gccggcgcac ccgcgccatc
      961 ctggacgcca tgtaccgctc ggcctacgaa tgcgatggcg gctgggtaga cgtggagccc
     1021 gagatggaat agcgcctgcg cgcgcggcac caccagacta ccgtcatcgg gagggggcgc
     1081 tgctcccagc cccctcccaa acaccctctc ccggcagcga taccgtggat gaagggaacg
     1141 gacgcggatg gagacgtgcg ggcgcgtccg gctatcctat ccccgcacac ccctgcttgc
     1201 ccaccggacc tccgccagcc ctgggggagg ggagctaccg cgcggctctc tcggagaagt
     1261 ccgatgtgat ccgacctgcg agccggcccg ctggatgagg cctggtatcc ggcaccaagc
     1321 cggaggattt gatgcgaaca ccttgtttcg gggggctttc ttcgttccgc aacttttgat
     1381 aaaatggagt caatcggatg agtggcgtgt ggtaggcatg gcgggcatca cttggggaag
     1441 gttgggagcc gatgtcatct gatcacgagg cgctgaagca ggccagtgag cgtctagaga
     1501 gcctggaacg cgagttgcgg cgcactcgcg aggagctgta tcgcctgatg gcgctggtag
     1561 accgcggccc cgccaccgtc ttcctctggc gcatcgcccc aagatggccc gtcgagtacg
     1621 tctccgcgaa catctcccgg tttggctacg accccgagga gttccgctct ggccgcatct
     1681 cctgggcaga gctgatccac cccgatgata cgccgcgcct ggatgccgag atcactcggg
     1741 ccatccagga cggcacgcac ggcctcgtcc tggaataccg cattatcacc cggagcggcg
     1801 aggtgcgctg ggtggaggag cacgccctcg gcctcctgga tgagcaccac agactgacgc
     1861 actaccaagg aatcatcctc gatatcacgg cgcgcaagcg cgccgaggcg gcgctgcttg
     1921 ccggcaagga ggagctcgag tcccgggttg ccgagcgcac cgcggatctg acgcgcgcca
     1981 acgagaaact ccggcgcgag attgcccagc ggcagcgggc catggaagcc ctacagctcg
     2041 atgagacacg cctggaagcc ttgcttgacc tcaacctcat gaccgaggcc tctgtcaagg
     2101 agatcaccca cttcgccatg gaggaggcgg tccgcctgac gaggagtacc ctcggctacc
     2161 tcgccttcgt caacgagtcc gagacggtga tgacgatgca cgcgtggtcc gagcgggcga
     2221 tgtacgactg cctcatcgcg gagaagcccc tgatctatcc ggtggagacc acggggcttt
     2281 ggggtgaagc ggttcgacag cggcggccga tcatcaccaa cgactaccag gcgccgaacc
     2341 cctggaagaa gggctatcca gagggccacg tggagctacg ccggcatatg aacgtgccga
     2401 ttatggaagg agggcgcgtc gtagccgtca ttggcgtggc caacaaggaa accgagtacg
     2461 acgatgccga tgtgcgccag cttcgcctgc tgggaaccgg catgtggcgc atcttggaac
     2521 gcaagcgcgt ggtagacgcg ctgaaggaga acgaagagcg gtatcgctcc ctcttcgcgg
     2581 tggagcccga cgccctcgtc ctcctcgatg cgcaaacagg ccagatcctg gaagcgaacg
     2641 actccgcgtg ccagctcttc ggttatgagc tcgaggagtt cctctctctg accgccctcg
     2701 atatctcggc cgagccggag aaaacgcgtg ccgtcatgga tgggtggcag ccaggccgga
     2761 ccgataaggt tccctaccgg aactcnnnnn nnnnncactt cggaaggatg gcaccatctt
     2821 cccgacggag attgcaatcc ggcatttcga gttgcagggg cgtcgcctcc tctatgccgc
     2881 ctatcgcgac atcacggccc gggtacgcgc ggaggccgag atggaagagg cacacgagcg
     2941 tcttctccgc gccgagatcg agaaaaagca gttctaccgg caggtcatcc gggctgtgac
     3001 caacaacaag ctgcacctgg tggacctggc ccagatcccc gtcgagggcg aactcgttct
     3061 ggaaacatcc ttcgacgatc ccgaatccta ccgtgcgctg cgccagcgcg tgcagcaggc
     3121 cgcgatcacg gcgggcatgc ccgaggagaa tgccgccgat ttcatgctcg ccgtctccga
     3181 ggcggcagcc aacgccatca aacacgccaa agatggccgc tgcgccctct atgtcactcc
     3241 ggagcgcgtc atcgctcgcg tgagcgaccg cggaaccgga atccacccgg agaacttgcc
     3301 ggcgaccatc ctgacgcccg gcttctctac caaggtctcg ctgggaatgg gctacacgct
     3361 catgctggag gtggccgacg agatgtggct gacgacgggt cccaccggca ctgtcgtcca
     3421 gctcgccaag tggatctatc ccgagcgcca cgtcaaggag ccggatctcc agatgtggaa
     3481 caacctctag ccccgtagcg cgctccgcgt gcgttacctc cgagccacgc gctcccgcca
     3541 ccggttcccg atatagaacg ggcgtgaccg atagcccccc tcgatagtca cccgcaggag
     3601 gttagcgccg ggcgcaaggc gcgtgcgctt ccccgtaaac agctccactg cgtccaccgg
     3661 gcgctctacg ggaatccgtg cctccaccgt cgtctttccg aggtgctcgt aatcggccag
     3721 caagacgagc atctcatcgc ccgagcggat cgccgagacg tggccggtgc tgccctgccg
     3781 ccactccttg gtgtcgcaca gcgtggaagt cgcaataatc tcctcgaagg gcgccaggag
     3841 gcgggccacc tgcgcctgcg ccgccagatc ctccgagcaa aggtgaacgg ggagaaagta
     3901 gagggtgccg gtcgcgccgt tgagcaactc ctccatcagc gtgtcgaaga ggcgtccaga
     3961 tggcgcctcg ccctcccaat cgagcgtgcc cggggaggtc caggcaatga tcgggcactc
     4021 tgggggaagg aggcgcttgt cggcccgaat gatctcggct gcggcggccg gccagcagcg
     4081 cacgtacacc tccgggttgt tgaactggca gcctgccgga tagagcttcg cgaaatcgaa
     4141 gacgttttga tagcggtacg ccggactggc atggtagaag ccgatgggcg ggggcgtctg
     4201 gcccaaatcg cgggccgcct gggcgatcgc cgcagccagc tccctcgcgg tctcggcgta
     4261 gagatccgtg gccagctcca ccggagtcac gccgcggtca tccagtagtc tccgacacgg
     4321 cgcgcacttc tggacgtgct cgagggcatg cccgaagcat tcgacatcga ggctgatcag
     4381 gtccggccgg cggcggcggt agcgctccac gatccgctcg atctctttct ggtagtactt
     4441 gccccggtaa gagaggcaga ggcgcttccc cggaggatcc gtctcgccat acgcttccgc
     4501 aaagtctggc tctcgcgccc acaacgcttc catgatgtgg aagggggaat cggtgaggca
     4561 cactttcagg ccggcgggcc ggg
//
LOCUS       DAOSCV010000278         4577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11912_length_4577_cov_3.723795, whole genome shotgun sequence.
ACCESSION   DAOSCV010000278 DAOSCV010000000
VERSION     DAOSCV010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_4577_cov_3.723795"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..915)
                     /locus_tag="PLY56_08065"
     CDS             complement(271..915)
                     /locus_tag="PLY56_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21476.1"
                     /translation="MMEKQSGRGFTLIELLVVIAIIAILAAILFPVFARARENARKTN
                     CASNVKQILLGIHQYAQDYDEVLPYASQWGSPLQPWHYYLQPYLKNSGILKCPSYPGA
                     AQGYGWNYQNFGYYSSGTGAFSNNPGRALAAVPVPAETILIADNPDAGMYGAGGIFAY
                     GPTQMNPSGAAANPGNAPARHGEGGNYGFVDGHVKWLAAQAAASQDRLWTVAED"
     gene            complement(1168..3135)
                     /locus_tag="PLY56_08070"
     CDS             complement(1168..3135)
                     /locus_tag="PLY56_08070"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ21477.1"
                     /translation="MELSGPPYLGAAYYPEDWPLDQIDDDIALMLEAGMNVMRVGEFA
                     WSRMEPREGEYDFEWLHLVIEKLGQAGIPTILGTPTCTPPAWLAERYPEILVMSDQGR
                     RAGHGGRRHACPNSPVYREHCARIVTRMAEEFGHDPRVIGWQIDNELFPAGPRGCCCP
                     VCHAKFQEAMRERFGTIEALNEAWGTHLWSQAYQDFRQLPIPRTDVRHHPSLLTAWMC
                     FQSDSYIEFAHAQAEILHRLATQPVGTDMMPTGALNYHQMHRKLDVVQFNHYNNPENL
                     WQAAFWMDLCRPIKKAPFWNTETSTCWNGGVTANGYKPSGFCRANSWLPIALGAEANL
                     YWLWRAHWSGQELMHGSVISSCGRPLHIFGEVREVARGFRAAADFLNGTRVARPKVAL
                     HFSGFAWWLFEHQPMVNGFRYLDQLLNGFYRPLLQAQIGVDVIDPAASLEPYTVVCSP
                     FLPALDEAGLRERLLPWLEAGGTWIAGPLTDIRTLDATKYRHAPYGSLEEWAGVTCRY
                     QIPGEPQSFALRWRDRSISGGSMWYDGLEPHHAEVVATYADGPLKGLAGVTCTPVGKG
                     QLLLLGTLPRGEDVREMVLGAGGYRAAEATPNLLVVPREGPGGRGLIVLDLGNQAGML
                     TLPQPATDLLTGRRHEGRIEVAPYGVMVLRY"
     gene            3411..4475
                     /locus_tag="PLY56_08075"
     CDS             3411..4475
                     /locus_tag="PLY56_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ21478.1"
                     /translation="MASDLSKLDPEMAGRDADDGIAWYNVQDWGVEGKGWEETEAYYD
                     RLPAKAKGVVRDPVWNLSRHSAGICFHFETDATEISARWVLRSSNLAMPHMPATGVSG
                     LDLYTRGDDGRWHWVGVGRPESGPKVQAKLASGLKPGYRAYRVYLPLYNGVTSVEVGV
                     DPKARFQPVPPRTERPIVFYGTSIVHGGCASRPGMCHPAILGRRLDRPVLNLGFSGNG
                     QMEPEVAALLAELDPCVYVIDCLPNMTAPLITERAVPLVETLRRAHPETPIVLVEDRT
                     FTNALFVPENRERCDSRRDAFRKAYARLLAAGVKHLYYVEGENLLGDDGEGTVDSSHP
                     TDLGFMRMADALEPVLAPLC"
BASE COUNT          820 a         1525 c         1481 g          751 t
ORIGIN      
        1 cgcgcaaccg tctgcgtgcg gcgaaggctg gcggcggcta cattctgagc agcgcctgct
       61 cggtggcgcc gcgcgtgcca ccggagaatc tgcttgctct gcgcgaagtg gttgaagcgg
      121 agggatggta ctgagggaga agcgatgcgc gcgggtcggc accaccaatg accttgggag
      181 ccacgccgcc gcgacgcctt ctcggtaaac caggcgggga gcgccttgtt cggcgctccc
      241 cgccttttcc catttctgcc ttccggagga tcagtcctct gccacggtcc acaggcgatc
      301 ctgcgaggcg gctgcctgcg cggccagcca cttcacgtgg ccatccacga acccatagtt
      361 gccgccctcg ccatgccggg ccggggcgtt gccgggattc gctgctgccc cggatggatt
      421 catctgcgtc ggcccgtagg caaagatacc gcccgcgcca tacatgcccg catccggatt
      481 atccgcgatg aggatcgtct ccgcgggcac cggaaccgcc gcgagggcac gccccggatt
      541 gttcgagaac gcccccgttc ccgaagagta gtacccgaag ttctggtagt tccagccgta
      601 tccctgagcc gcgcccgggt acgacgggca tttcaggatg cccgagttct tcaggtaggg
      661 ctgcagatag tagtgccagg gctgcaacgg cgagccccac tgcgaggcgt aggggagtac
      721 ctcatcgtaa tcctgagcat actgatggat gcccaggaga atctgcttga cgttggacgc
      781 gcagtttgtc ttccgcgcgt tctctcgcgc ccgagcgaaa acggggaata agatcgccgc
      841 gagtatagcg ataatcgcta tgacaacgag cagttcgatg agcgtaaaac cccgaccact
      901 ctgcttctcc atcatcttct acctccacgt gtgtcgggaa agcttgtcac ctgccggcac
      961 gcacctcacc agcggtctgg gggcagatac gcacggtgct cgccttccat tactctcatg
     1021 ataattgccc gcgcctggcc tgtcaagggg aaaagcgaaa gggaacgcgc caggaagaat
     1081 cagtgcgcgt agaagacgcc cttatgggcc ggccagtgcg gcgcggtgtt cgctcgcccg
     1141 ccactgaccg gtccgtcggc gaggccctca gtagcgcagg accatcaccc catagggagc
     1201 cacttcgatg cgcccctcgt ggcgtcttcc cgtcaagagg tcggtggccg gttgaggcag
     1261 tgtcagcatc cccgcttggt ttccgaggtc gagaacgatg agcccgcgcc cccccggacc
     1321 ctctcggggc accaccagca ggttcggcgt ggcttcggcc gcgcgatacc cgcccgcgcc
     1381 caagaccatc tcccgaacat cctcgccgcg cggcagcgtg cccaggagca gcagctgccc
     1441 cttgcctacc ggcgtgcacg tgacccccgc cagccccttg agcggcccat cggcgtaggt
     1501 agccaccact tcggcatggt gcggctccag gccatcatac cacatcgagc cacccgagat
     1561 ggagcggtcg cgccagcgaa gggcaaagct ttgtggctct cccgggatct gataccggca
     1621 ggtgaccccg gcccactctt ccagggagcc atacggcgca tgacgatact tcgtcgcatc
     1681 cagcgttcgg atgtcggtca gcggaccggc gatccaggtg ccgcccgctt cgagccacgg
     1741 caacaggcgc tcgcgcagac cggcctcatc cagcgccggc aagaagggag agcagaccac
     1801 ggtgtacggc tccagagaag ccgcgggatc gatcacatcg acgccgatct gggcctggag
     1861 cagcggtcga tagaaaccat tcagcaactg gtcgaggtag cggaacccgt tcaccatcgg
     1921 ctggtgctcg aagagccacc aggcaaaccc ggagaagtgg agggccacct tcgggcgagc
     1981 gacgcgggtg ccgttgagga agtcggccgc cgcgcggaag ccgcgggcga cctcgcgcac
     2041 ctcgccgaag atgtgcagcg gccggccaca gctggagatg acggagccgt gcatcagctc
     2101 ctggccgctc cagtgcgcgc gccacagcca gtacaggttg gcctctgcgc cgagggcaat
     2161 cgggagccag gagttggccc ggcagaagcc ggacggctta tagccattcg ccgtgacgcc
     2221 gccgttccag caggtggagg tctccgtatt ccagaagggc gctttcttga taggccggca
     2281 aagatccatc cagaacgccg cctgccacag gttctccggg ttgttgtagt ggttgaactg
     2341 gaccacatcc agcttgcggt gcatctgatg gtagttcagc gcgcccgtgg gcatcatatc
     2401 ggtgccaacc ggctgtgtgg ccaggcggtg gagaatctct gcctgcgcgt gggcaaactc
     2461 aatataggaa tccgactgga agcacatcca cgcggtgagg agcgacggat gatgccggac
     2521 gtcggtgcgt gggatcggga gctgccggaa atcctggtag gcctggctcc acaggtgggt
     2581 gccccaagcc tcgttgagcg cctcgatggt gccgaaacgc tcgcgcatcg cctcctgaaa
     2641 cttggcatgg cacaccgggc aacaacagcc ccgggggccg gcgggaaaca gctcgttgtc
     2701 gatctgccag ccgatgacgc gcgggtcgtg cccgaactcc tcggccatgc gcgtgacgat
     2761 acgcgcgcag tgctcccggt aaacggggct gtttgggcag gcgtgtcgcc ggccgccgtg
     2821 tccagcgcgc cgcccctgat ccgacatcac cagaatttcg gggtatcgct ccgccagcca
     2881 ggccgggggc gtgcacgtgg gagtgcccag aatcgtcgga atccccgcct ggcccagctt
     2941 ctcgatcacg aggtggagcc actcaaaatc atactctccc tcgcgcggct ccatgcggct
     3001 ccaggcgaac tcgcccacgc gcatgacgtt catccccgcc tcgagcatca gggcgatatc
     3061 gtcgtcaatc tgatccaggg gccaatcttc cggatagtag gccgcaccca ggtacggcgg
     3121 acctgatagc tccatgcaaa gctcctctcc cacacggcat ccccctctca ttaccacgcc
     3181 gcccgccggg gatccttgtc gagcccacgg acaaccaccg gacaacggaa ggcaggcagg
     3241 aacagcctgc ttcaccaggg aagtacgccg cgggctgaac gctcgtcgat accccgcgag
     3301 gcggaaagcc atcttattgt tgcgtgagac agaacggccg gcgcccgcgc aggcgtgccc
     3361 aaggcgatct ggagggcggg gccgttgcag ggcaggaacg aggagacccc atggcaagcg
     3421 atctgagcaa actggacccg gagatggcgg gacgcgacgc tgatgacggc atcgcctggt
     3481 acaacgtcca ggattggggc gtggagggca aaggctggga ggagacggag gcctattacg
     3541 accggcttcc ggcgaaggcg aaaggcgtgg tgcgcgatcc ggtgtggaac ctcagccgac
     3601 actcggcggg gatctgtttc cacttcgaaa cggatgccac cgagatctcc gcgcgctggg
     3661 tgttgcgctc gagcaacctc gcgatgccgc atatgccggc gaccggcgtg agcggcctcg
     3721 atctgtatac ccgtggcgat gatggccgct ggcattgggt cggggtgggc cgccccgaga
     3781 gcggacccaa ggtgcaggcg aagctcgcct ccggcctgaa gcccggctac cgggcttacc
     3841 gcgtctatct ccccctctat aacggggtaa cttccgtgga ggtgggcgtc gatccaaagg
     3901 cccgcttcca gccggtgcct ccgcgcaccg aacgcccgat tgtcttctat ggcacctcta
     3961 tcgtccacgg cggatgcgca tcccgacctg gcatgtgcca cccggcgatc ctgggccggc
     4021 gcctcgaccg acccgtcttg aacctcggct tctccggcaa tggccagatg gagccggaag
     4081 tggccgcgct gcttgccgag ctggacccgt gcgtctacgt catcgactgc ctgccgaaca
     4141 tgacggcgcc cctcatcacc gagcgcgccg tgcccctggt agagacgctg cgccgtgccc
     4201 acccggaaac gccgattgtc ctggtggagg accgcacctt cacgaacgcg cttttcgtgc
     4261 cggagaaccg cgagcggtgt gatagcagac gagacgcctt ccgcaaggca tacgcccggc
     4321 tcctcgccgc cggcgtgaag cacctgtact acgtggaggg cgaaaacctg ctgggcgacg
     4381 atggcgaggg cactgtggat agctcgcacc caaccgatct cggcttcatg cgcatggccg
     4441 acgccctgga gccagtcttg gcaccactct gctgaaccac gtttcgatgg ggcgctccct
     4501 cccctcgccg ggtgggggag cgccctggag tgccgtgccc ggcttccggg cccccaaaag
     4561 gagcccccgg gaagagg
//
LOCUS       DAOSCV010000279         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11918_length_4576_cov_3.265207, whole genome shotgun sequence.
ACCESSION   DAOSCV010000279 DAOSCV010000000
VERSION     DAOSCV010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11918_length_4576_cov_3.265207"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLY56_08080"
     CDS             complement(<1..214)
                     /locus_tag="PLY56_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21479.1"
                     /translation="MSEYRVGIIGCGRPHKTEGSTGYGQAHRHMLGYQASPEARLVAV
                     ADIVEENARAFQEPYGAEKRYTDYREM"
     gene            complement(276..1685)
                     /locus_tag="PLY56_08085"
     CDS             complement(276..1685)
                     /locus_tag="PLY56_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ21480.1"
                     /translation="MASVHSVSAPKPHGAIPSERQLRWQAMEVTGFIHFTVNTFTDKE
                     WGYGDESPEIFAPTAFDAGQIAGVAAEAGMKGLILTAKHHDGFCLWPSAYTDHSVKHS
                     PWRGGQGDVVRELATACQEQGIGFGIYLSPWDRNHPRYGHPDYLTYYRNQLRELLSNY
                     GPLFEVWFDGANGGEGYYGGARENRRIDKERYYDWETTWSIVRELQPEAVIFSDAGPD
                     IRWVGNESGVAGDPCWGTISPDGMAPGLADPKVLNTGHRNGSHWRPAEVDVSIRPGWF
                     YHAHEDDQVRSPENLLQVYYESVGRGCNLLLNLPPDRRGLIHERDVASLREWRRILDA
                     TFATDLARGARVTASNTRGNDPRFAPTHVIDGRRDTYWATDDDVRTPELVLDLGRHAT
                     FNVVCVREYLPLGQRVDSIALDRWDGERWVEFASATGIGAQRLIRLPERITTDRVRLR
                     VTGSPVCPAISELGLFAEP"
     gene            1973..>4576
                     /locus_tag="PLY56_08090"
     CDS             1973..>4576
                     /locus_tag="PLY56_08090"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21481.1"
                     /translation="MAPGSAQPVDRDGDGMSDVAEAALGTDPKTAEAFTTIVTRKPSE
                     KVADKSRFVTEIALANAGGDRFVWRVQFAEPWPVENANVLLYVDSDSDAKTGRPGHGC
                     EAMIRVTNGEGGVTAYAPDGQVITGSLPRAFVDGRYVYVAHDMPLKQENGQSVFRLML
                     LSETWQPHTGVDSIPYFVARGPAMSDRPKLKLDSDLTESQGMEQTYGPRTVDPLLNAK
                     ENVRLPIFQCETQGFRFVPSEYRADNVIRTGSPARITAKVPRAGSFYPGFVIHDEAGR
                     EVIGLFVNGERQGVAVASVDDNDQRLFFLSRPISLKQGDTIELRVLSSEGPYRIEDLV
                     LLAKKPAARPLRYEFRHLEAGENRITWITTWPAACVVELDNGQRIQEPAPVNNHRVYV
                     PGMKPGQTIRYRITTVTREGKPVATAWQRHTWKPVVEPATKRAGSVVLRVEAPTGTTL
                     KGWPVTSGVPFPQGVLGSADRLALADAAGKPVPLQATVTARWPDGSVKWVLLDFRHSG
                     PSTTYQLRYGPQVRRDALAQASVRPPTLGALTLMDANGKQSTVQLAATRVEEQGSLRT
                     CFYGSGQLKDGDVSRLAYEVRAHSYPGTPWVRVLLTFTNDHPGSEFQTLRSLKWDVPV
                     KTAQSGFVRQHTDDRYAASTGEGKRWSGPLGALFVRDFWQNYPLDLEVGPQGAAFWLL
                     PPLKADEYDWAKGTVDEHRLFYWFQALDNAGGYKLRQGVSKTHEVWIGLDGAAPQHDR
                     PLMAACPPSWYADSKVFGDFAIADAKREVVKEYDAKVAETLEGYLQNRERNREYGALN
                     FGDWWGERVINWGNIEYDTQHAFFLQFARSGDLRFFQAGEEAERHNRDVDTIHAHTND
                     YRVGAVYSHCIG"
BASE COUNT          781 a         1456 c         1544 g          795 t
ORIGIN      
        1 gcatctcccg gtaatcggtg tagcgcttct cggcgccgta cggttcctgg aacgcgcgcg
       61 cgttctcctc aacgatatcc gccacggcga cgaggcgcgc ttctggcgac gcctgatagc
      121 ccagcatgtg ccggtgtgcc tgcccgtagc ccgtggatcc ttccgttttg tgcggtcgcc
      181 cgcacccgat gattccaacg cgatactcgc tcatggaact cctcccggcg tctctttctc
      241 cacgcctggc ggccctcctt gggatggatg cgccgtcatg gctccgcgaa aagcccgagc
      301 tccgagatcg ccgggcagac gggcgacccg gtgacgcgca ggcgcactcg gtcggtggta
      361 atccgctccg gcagccggat caggcgctgc gcgccgatgc cggtcgcgct cgcgaactct
      421 acccaccgct cgccatccca acggtccagg gcaatcgaat cgacgcgctg gccaagaggg
      481 agatactccc ggacgcacac cacgttgaaa gtggcgtgcc ggcccaggtc caggacgagc
      541 tccggtgtcc gcacgtcatc atcggtcgcc caataggtat cgcgccggcc gtcgatcacg
      601 tgggtgggcg cgaagcgcgg gtcgttgccg cgcgtgttac tcgcggtcac gcgcgcgccg
      661 cgggccaggt cggtggcgaa ggtggcgtcc aggatgcgcc gccattcgcg gagcgaggcg
      721 acatcgcgct cgtggatgag gccgcgccgg tccgggggca ggttgaggag gaggttgcac
      781 ccgcggccca cggactcata gtagacctgg agcaggttct caggcgagcg cacctggtca
      841 tcctcatggg cgtggtagaa ccacccgggg cggatggaga catccacttc cgcgggccgc
      901 cagtgcgagc cgttgcggtg gcccgtgttc agcaccttgg gatcggccag tccgggggcc
      961 atcccgtccg ggctgatggt gccccagcag ggatcgcccg cgaccccgga ttcgttaccg
     1021 acccagcgga tatccgggcc ggcgtcgctg aagatcaccg cctcgggttg cagctcgcgg
     1081 acgatgctcc acgtcgtctc ccaatcgtag tagcgctcct tgtcaatccg gcggttctcg
     1141 cgggcgccgc cgtaatagcc ttcgccgccg ttggcaccat caaaccacac ctcgaagagc
     1201 ggcccatagt ttgagagcaa ctcacggagc tggttgcgat aataggtaag gtagtccggg
     1261 tggccatacc gcgggtggtt gcggtcccag ggcgagaggt agatcccgaa gccgattccc
     1321 tgctcctggc aggcggtggc gagttcgcgg acgacatcgc cctgcccgcc gcgccagggc
     1381 gaatgcttca ccgagtgatc ggtgtaggcg cttggccaga ggcagaaacc atcgtggtgt
     1441 ttggcggtga ggatcagccc cttcatgcct gcctcggcgg ccacaccggc gatctgtccg
     1501 gcatcgaacg ctgtcggcgc gaagatctcc ggggactcgt cgccatagcc ccactccttg
     1561 tcggtgaagg tgttcaccgt gaaatggatg aagccggtga cttccatcgc ctgccagcgg
     1621 agctgccgct cgcttggtat ggcgccgtgg ggtttcggcg cggagacgga gtggacgctt
     1681 gccatctgct caacaacctt ccctgggtga tgaccggctg ccgcgcgggc cttcgagccg
     1741 gcctggccga gatcccattg gcttccgccg cggtctcatc ctgcaccgga gggcggggct
     1801 ccggcaggaa aacctgctct tacttggaac agagagacag catgggcgcg cggccagcag
     1861 gcttggtgcc cgtaggatcc gaagaccgga aggcaggagg accatgtacc ctcgaaactc
     1921 tcttcgttgg tggtcggtac ccatcctggc gctcttgttc ttggtgccca ccatggcacc
     1981 agggagcgca cagcccgtcg atcgtgatgg cgatggcatg agcgacgtgg ccgaagcggc
     2041 gcttgggact gaccctaaga ctgcggaggc gttcaccacc atcgtgacac gcaagccctc
     2101 tgagaaggtg gcggataagt cacgcttcgt caccgagata gctttggcca acgccggggg
     2161 cgaccgtttt gtctggcgtg tccaatttgc cgagccgtgg ccggttgaga acgcgaacgt
     2221 gctcctctat gttgacagcg acagcgacgc gaaaacaggg cgccccgggc acgggtgcga
     2281 ggcgatgatc cgcgtgacga acggcgaggg cggggtcacg gcctatgccc ccgatggtca
     2341 ggtgatcacc ggatcgttgc cccgagcctt cgtggatggc cgctacgtgt atgttgccca
     2401 cgacatgccg ctgaagcaag agaatggaca gtctgtcttt cgcctcatgc tgctgtcgga
     2461 gacctggcag ccgcacacgg gcgtcgacag cattccctac ttcgtggcgc gagggcccgc
     2521 gatgagtgac cgccccaagc tgaagctcga ttcggacctc acggagagcc aggggatgga
     2581 gcagacgtac gggccgcgca cagtggaccc gctgctcaac gccaaggaaa acgtgcgtct
     2641 tcccatcttc cagtgtgaga cccaggggtt ccggttcgtg cccagcgaat accgtgcgga
     2701 taacgtcatc cgtacggggt cgccggcgcg aatcacggcg aaggtgccgc gcgcgggcag
     2761 cttctaccct ggttttgtca tccacgacga ggccggacga gaggtgattg gcctcttcgt
     2821 caatggcgag cgtcaggggg tagcggtcgc ttcggtcgac gacaacgatc agcgcctctt
     2881 cttcctgtcc cggccgatct cactgaagca gggtgatacg atcgagctgc gcgtcctctc
     2941 ctcggagggg ccctaccgga tcgaggacct ggttctgctg gccaagaagc ccgcggccag
     3001 gccactgcgc tacgagttcc ggcatctaga agccggcgag aaccggatca cctggatcac
     3061 cacgtggccg gcagcgtgcg tggtggagct ggataacggc cagaggatcc aggagccagc
     3121 tccagtcaat aaccaccgag tctacgtgcc ggggatgaaa cccggtcaga cgatccgata
     3181 ccggatcacg accgtgaccc gcgaggggaa gccggtcgcc accgcgtggc agcggcacac
     3241 ctggaagccc gtggttgagc ctgccacgaa gcgcgcggga agcgtggtgt tgcgcgttga
     3301 ggcgcccacc gggaccactc tgaaggggtg gccggtcacg agtggagtgc ccttcccgca
     3361 aggcgtgttg ggttctgcgg accgcctggc gctggccgac gcggcaggca agccggtccc
     3421 gcttcaggca acggtgacgg cgcgctggcc ggacggcagc gtgaagtggg tgctgctcga
     3481 cttccggcac tcggggccga gcacaaccta ccaacttcgc tatgggccgc aggtgcgtcg
     3541 tgatgcgctc gcacaagcct ccgtgcgtcc gccgacgctt ggcgcgctga ccctgatgga
     3601 tgcgaacggc aagcagtcta ccgtgcagct cgcagccacg cgagtcgagg agcaagggtc
     3661 gctgaggact tgcttctacg gctctgggca gctgaaggat ggcgacgtga gccgtctcgc
     3721 ctatgaagtg cgcgcgcaca gctatccggg cacgccgtgg gtgcgagtgc tcctcacctt
     3781 taccaatgac caccctggaa gcgagtttca gacactgcgc agcctgaagt gggacgtgcc
     3841 cgtgaagacg gcgcagagcg gcttcgtgcg ccaacacacc gacgatcgtt acgcggcgtc
     3901 cacgggcgag ggcaagcgct ggtctggccc gctgggcgcg ctcttcgtgc gcgacttctg
     3961 gcagaactac ccgctcgacc tggaggtcgg acctcagggt gcggcgttct ggctgcttcc
     4021 gccgctaaag gcggatgagt atgactgggc caagggcacg gtggacgagc accggctctt
     4081 ctactggttc caggcgctgg ataatgccgg cggctacaag ctgcgccagg gcgtctcgaa
     4141 aacacatgag gtgtggatcg ggcttgatgg cgcggcgccg cagcacgacc gcccgctcat
     4201 ggccgcgtgc ccgccctcct ggtatgccga ttcgaaggtc ttcggcgatt tcgctatcgc
     4261 ggacgcgaag cgagaggtcg tcaaggagta cgatgccaag gtggcggaga ccctggaggg
     4321 ctaccttcag aaccgcgagc gcaaccgcga atacggcgcg ctcaactttg gcgactggtg
     4381 gggcgagcgc gtgatcaact ggggcaacat cgagtacgac acccagcacg ccttcttcct
     4441 ccagtttgct cgttccgggg atctccgttt cttccaggcc ggcgaggagg ccgagcgcca
     4501 caatcgcgat gtagatacga tccatgcgca tacgaatgac tatcgcgtgg gtgccgtcta
     4561 ttcccactgc atcggc
//
LOCUS       DAOSCV010000280         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11920_length_4575_cov_3.621681, whole genome shotgun sequence.
ACCESSION   DAOSCV010000280 DAOSCV010000000
VERSION     DAOSCV010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11920_length_4575_cov_3.621681"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLY56_08095"
     CDS             <1..290
                     /locus_tag="PLY56_08095"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ21482.1"
                     /translation="PLTPEEGALVRIYHSIPEVVEPEITEEVTWVNEVHIPIRLFEGR
                     FGPLGDLAGQEWRGNLYKCGDETSHPHWATWAPIEGGNFHQPRFFGPLRFE"
     gene            complement(404..1648)
                     /locus_tag="PLY56_08100"
     CDS             complement(404..1648)
                     /locus_tag="PLY56_08100"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ21483.1"
                     /translation="MGTLKIGCGSAVITPGLGAHLAGYFNERRATAVHDDLFARALVF
                     DDGTTRAALLACDVICLPEEEVARVREAIETRTGLSGSSVMVSATHTHTGPQTRAFQL
                     PEDAEPTREWLKTFPERAAGAVVEALEKMEEVQVYHGTAFEDRIAFNRRYRMKDGTVR
                     TNPGYQNPDIVGPAGPIDPEVGVLAYTRADGTRKALLVHYTCHLDNVSGTEISADYPG
                     YLAARLRERLEDQPFVFYAQGACGDINHCDFRSPYPRKGHEHSRWMGETLAGDVCEAL
                     RDLKPLEGTPVKIASKMVPLPVREGVEPLATEAEVQAIRVGDVGFVSIPAEYFVEHQL
                     DIKARSPFQRTFVCELANGWVGYVPTRKAFEENMRHVSWERMTGFDHMGYEVRSALSR
                     GFAPGVGEALADGAVELLQSLR"
     gene            complement(1756..2625)
                     /locus_tag="PLY56_08105"
     CDS             complement(1756..2625)
                     /locus_tag="PLY56_08105"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21484.1"
                     /translation="MGWPVIMHVNYCEQGQSLREICFKAVEWGFDGVEFRRKRTGVEE
                     EPEAYLDALAEAVQASGLKHVLFGYPTANLMTPDAGEREREVENAIAFYRSARQRFPI
                     LLCNAFSGPLLNPDKNIPYREYQKQGSGIATEEHYQWAAEGFRVLAPVAEELGFRFAF
                     ELHMGYLHDLPASAAKLIEMIGHPAVGANLDYGNIIYFPERPTLAESIRTLAGRLYYV
                     HLKNSVALPTGCRLPTALADGEINHREYLRLLVEVGFDGPICIEAPRPGDREWFAQQD
                     LAYIRSVIRDIAS"
     gene            2778..3530
                     /locus_tag="PLY56_08110"
     CDS             2778..3530
                     /locus_tag="PLY56_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ21485.1"
                     /translation="MSLGWEAFIPVHLKKIRLLPDCYPAVDCYPFNVPALRETREIPL
                     TTPVTFFVGENGTGKSTLLEAVARRCGIHIWTSEYTPRMEHNPHEEKLHLYMAVEWTA
                     GSVPGSYFSARIFRTFAEMTEVVAANDPGQLRYYGGKSLLSQSHGQSLLSYFGARYQL
                     RGLHFLDEPETALSPRNQIQLLRLLRDMARAGHAQFLVATHSPILLACPGATLYSLDS
                     SPIQAIAYEETEHYRLCHEFMADPQAYLASIQ"
     gene            3594..3803
                     /locus_tag="PLY56_08115"
     CDS             3594..3803
                     /locus_tag="PLY56_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21486.1"
                     /translation="MTFSARQWRKVWEQLYNSGEMNLSGRIAHEVGHIWNGDNWDEQV
                     TIDFSAESFERVRDAANKAGVLVNW"
     gene            4025..>4575
                     /locus_tag="PLY56_08120"
     CDS             4025..>4575
                     /locus_tag="PLY56_08120"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ21487.1"
                     /translation="MGTLETQIEQYAAMAIQVGVNLQPGQTLFIGGPAGAPIERADFV
                     RLLATKAYDAGASRVHVLWEDEWLTRLTLERASEEALRTFPAPLARWFEEAAAEGSAF
                     LRVSARDPDLLDGVETRRVTESQRAMMAAMAPYMEYTGAMRVSWSIVCAATRAWANKV
                     FPQLPEAERVDALWRALLQAARIS"
BASE COUNT          767 a         1556 c         1460 g          792 t
ORIGIN      
        1 cgccgctcac gcccgaggag ggcgctctgg tccgcatcta ccactccata ccggaggtcg
       61 tcgagccgga gatcaccgag gaggtgacgt gggtgaacga ggtccacatc ccgatccggc
      121 ttttcgaagg ccggttcggg cctctcggtg atctggccgg ccaggagtgg cgcggcaacc
      181 tctacaagtg cggtgatgaa acgtcgcacc cgcattgggc cacgtgggcg cccatcgagg
      241 gcggcaattt ccaccagccg cgcttcttcg gcccgctgcg gtttgagtga cgggccagac
      301 tcagcggggg agggatccac gacccatgga tccctcgccc ctcagattgc cattccgtct
      361 tcctgacgcc tggcaggcgc ccggctggtc gccagatctg gcgtcagcgc aggctctgca
      421 ggagctccac ggcgccgtct gccagcgcct ctccaacgcc aggcgcgaag ccgcgagaga
      481 gcgccgagcg cacttcgtag cccatatggt cgaagccggt catccgctcc caggagacgt
      541 ggcgcatatt ctcctcgaac gccttgcgcg tcggcacata gcccacccaa ccgttggcca
      601 actcgcagac gaaagtgcgc tggaagggcg accgcgcctt gatatcgagc tggtgttcca
      661 cgaagtactc agcggggatg ctgacgaagc cgacgtcgcc cacgcggatc gcctggactt
      721 ccgcctcggt ggcaagcggc tccacgcctt cgcgaacggg gagcggcacc atcttgctgg
      781 cgatctttac cggggtgccc tccagcggct tgagatcacg caacgcctcg cagacgtcgc
      841 cggcgagcgt ctcgcccatc caccgcgagt gttcgtggcc cttccgcggg taggggctgc
      901 ggaagtcgca gtggttgata tcgccgcagg cgccctgcgc gtagaagacg aacggctggt
      961 cctccagccg ctcgcggagc cgcgcggcca gatagccggg gtaatccgcc gagatctcgg
     1021 tgccactcac gttgtcgaga tggcaggtgt agtggacgag gagcgccttc ctcgtgccat
     1081 cggcccgggt gtaggcaagg acgccgacct caggatcgat ggggccggcc ggcccgacga
     1141 tatccgggtt ctggtagccc gggttggtac gcacggtccc atccttcatc cgatagcgcc
     1201 ggttgaaggc gatgcggtcc tcgaacgcgg tcccatggta gacctggacc tcctccatct
     1261 tctccagcgc ctcgacgacc gcgcccgccg cacgctccgg gaaggtcttc agccactcgc
     1321 gcgtcggctc tgcatcttcg ggcagctgga aggcacgcgt ctgcggcccc gtatgcgtgt
     1381 gcgtggcact caccatcacg gagctgcccg aaagccccgt gcgcgtctca atcgcctcgc
     1441 gcacccgggc cacctcctct tccgggaggc agatcacgtc acaggccaag agagcggctc
     1501 gggtggtccc atcgtcgaag acgagggccc gagcgaacag gtcatcatga acggcggtgg
     1561 cccgccgctc gttgaaatag cccgccagat gcgcgcccag accaggcgtg atgacggcgc
     1621 taccgcagcc aatcttcaga gtacccatgc tatctcctca tgccgttccc agcatagacg
     1681 gcctgcccct cttgctcggg gcggcaggat tgcccgccgg agccaatcgc tttgcgcggc
     1741 cattcggggg cccgctcagg acgctatgtc gcggatcacc gaacggatat aggccaggtc
     1801 ttgctgcgcg aaccactccc gatcaccggg gcgcggagcc tcgatgcaga tcgggccatc
     1861 aaacccgacc tcgacgagaa gccgcagata ctcgcggtgg ttgatctcac catccgcgag
     1921 ggccgtggga agccggcatc ccgtgggcaa cgccacggag ttcttcagat gcacgtaata
     1981 gagacgcccg gccagagtgc ggatcgactc ggccagggtc gggcgctcgg ggaagtagat
     2041 gatgttgccg tagtccagat tggcgcccac ggcgggatgg ccgatcatct cgatcagctt
     2101 cgcggcgctc gccggaaggt catgcaggta tcccatgtgg agctcgaaag cgaagcggaa
     2161 accgagctcc tccgcgacgg gtgccagcac gcgaaacccc tccgccgccc actggtagtg
     2221 ctcctccgtg gcgataccgg agccctgctt ctggtattcc ctataaggga tgttcttgtc
     2281 cgggttcagg agcgggcccg agaaggcgtt gcataggagg atcgggaagc gttgcctcgc
     2341 ggaccggtag aacgcgatgg cgttctctac ctcccgctcg cgctcgcccg cgtccggggt
     2401 catcaggttc gcggtgggat agccgaacag gacgtgcttg agacccgagg cctggaccgc
     2461 ctcagccagg gcatccaggt aggcttccgg ctcttcttcc acgccggtgc gcttgcgccg
     2521 aaactccaca ccgtcgaagc cccactccac ggccttgaag cagatctctc gaaggctctg
     2581 cccctgctcg cagtaattga catgcataat cacgggccaa cccatgaagc gtctccttcc
     2641 tcactctgcg ccggcatacg caagcttggt tagccctttg cacgcgattc tcctgcgcga
     2701 aaagacgcca ggggcatggc tatacattga cacgaccgcc ttgcctaggc tatactccag
     2761 gaggccgcat ccattatgtg agccttggtt gggaggcatt catcccggtg catctaaaga
     2821 agatccgcct gctgccggat tgctatccgg cggtggactg ctatccgttc aacgtgccgg
     2881 cattgcgcga gacgcgtgaa atcccgctca cgacgccggt gaccttcttt gtcggggaga
     2941 acggcaccgg caaatccacg ctcctggagg ccgtcgcccg caggtgcggc attcacatct
     3001 ggacgtcgga atacacccct cggatggagc acaaccctca tgaagagaag ctccacctct
     3061 acatggccgt ggagtggacg gctgggagcg tgcccggctc ttacttctcg gcgcggatct
     3121 tccgcacttt cgccgagatg accgaagtgg tcgccgccaa cgacccgggc cagctccgtt
     3181 actacggggg gaagtcgctc ctctcccagt cgcacggcca gtcgctgctc tcctacttcg
     3241 gggcgcgtta ccagcttcgc gggctgcatt tcctggatga gccggagacg gcgctctcgc
     3301 ctcgaaacca gatccagctc ctccggctgc tccgcgacat ggcccgggcc ggccacgcgc
     3361 agttcctggt cgctacccac tctcccatcc tgctcgcctg cccgggggca acgctctata
     3421 gcctcgattc ctctccgatc caggccatcg cctatgagga gacggagcac tatcggctgt
     3481 gccatgagtt catggccgac ccccaggcct acctggcctc catccagtag ctcgccggaa
     3541 agcgcgcata cgggtcgctc tgccggggta tagagcccgg ggagggtatc gacatgacgt
     3601 tttcggcgcg ccagtggcgc aaagtctggg aacagctcta caactccggc gagatgaacc
     3661 tctccgggcg catcgcccat gaggtgggcc acatctggaa cggcgacaac tgggacgagc
     3721 aggtcaccat cgatttcagc gccgagtctt tcgagcgcgt ccgagatgcc gccaacaagg
     3781 cgggagtctt ggtgaactgg taggtctcat cagcaggccg gcggggccgg tgccggcccc
     3841 gtctcccgcc catccctttg gcgaggcact cccctctcac cgatccgccc ccgcgaagat
     3901 gccccgcgcc tctcccgagt gttgacgaga cgcccgcttc tgcggtaagc tagcgttgcc
     3961 cggcatccac cccgctctgc cgggcgcggc tctggcgtgt ccagagccgg cgggaggagg
     4021 atggatggga acactggaga ctcagatcga gcagtacgcc gcgatggcga ttcaggtggg
     4081 ggtgaacctc cagcccgggc agacgctttt catcgggggc ccggcgggag cgcctatcga
     4141 gcgcgcggac ttcgtgcgcc tgcttgccac caaggcgtat gatgccggtg cgagccgcgt
     4201 ccacgtgcta tgggaggacg aatggctcac ccggcttacc ctggagcgcg cgtcggagga
     4261 ggccctgcgc acatttccgg cgcccctcgc gcgctggttc gaggaggccg ccgcagaggg
     4321 ctctgccttc ctgcgcgtct cggcgcgcga cccggacctc ctggacggcg tggagacacg
     4381 acgcgtcacc gaatcgcagc gggcgatgat ggccgcgatg gccccctaca tggagtacac
     4441 cggggcgatg cgcgtcagct ggtcgatcgt ctgcgcggct acgcgggcct gggccaacaa
     4501 ggtgttcccc cagctccccg aggctgagcg cgtggacgcg ctctggcgcg ccctcttgca
     4561 ggccgcgcgc atctc
//
LOCUS       DAOSCV010000281         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11921_length_4575_cov_3.526106, whole genome shotgun sequence.
ACCESSION   DAOSCV010000281 DAOSCV010000000
VERSION     DAOSCV010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11921_length_4575_cov_3.526106"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLY56_08125"
     CDS             <1..1158
                     /locus_tag="PLY56_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21488.1"
                     /translation="EPWYSTFWGANVRGPDYRDDPEVRKIILENERREMRFMKSLGLN
                     SVAFGDDLRKDLELADGRVRLKPDNRFALWMDLYKEAGMGPMPFYGFQPLGAGNLLAW
                     LEREKLKERFTPQWNTAYRSFVEECQRIGKERGWPEILWYISDELSNHGEEGAKLGVE
                     LAKALKGMPGVRTIASMNGPWEHIMVPHLNISMPNIAFPITEETVKKVRDSGSELWLY
                     NCGDERLNLGLYPWRVKAGGRFQWHYRYINADPWDDLDGAGADTQYCLSLPGPDGPVP
                     AVKSQMVREAVDDHRYIATLEQAIARARSDAKKQAAVAKATRFLEELRTRIPVDFRTL
                     VGYQVDPRAAGASVGGEFKNTDALDRVRWAVAQLILELQTAGGGATTGKGK"
     gene            1158..2534
                     /locus_tag="PLY56_08130"
     CDS             1158..2534
                     /locus_tag="PLY56_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HOJ21489.1"
                     /translation="MNAPAHGRIAALSRWRALLFGGLLLLAPAVHQPAQAEAALPRIA
                     VARASSPVVIDGRLDDPAWNTATPHSNFRALAARGRETAPPEETIFRVLFDGEALFLG
                     IVCREPQIGNLVANAEGPDGRIWLDDSVEVLLQPTGRFYYQFGINSRGTRFDARMPVA
                     DDISEKEVAGGALWDGVWESAVSEGDGEWRIELRIPLATLDLGPESLPAWRLNIGRTA
                     ARRMEYSAWAPVEKGFHDLPRFGYLEGIVIDSARFPLDGSALAFPPLLLGRNEIQLSL
                     PARQPGRFRALTSLREWSPDGSATRQTAGEAPVTGGKWSLAFPVPVTRAGVLYELGLE
                     IQDEVTNTPVLLRKHVFRAPDPFDASLDWMISYGSDRTARATMTLAVAESSARGKVEV
                     ALWDGGAKPQQRITRNVSKPGTLHVALPLAKLPDGFYRVEVSATLADIGRLSKELRFY
                     KTSGPFDQ"
     gene            3119..3742
                     /locus_tag="PLY56_08135"
     CDS             3119..3742
                     /locus_tag="PLY56_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOJ21490.1"
                     /translation="MASPDEVKREELWNSISHGIGIPLGIAALVVAVAFASLERDIWK
                     IVSVSIYGTTLVLMYTASTVYHAISGKAKRVWNLLDHSSIFLLIAGTYTPVILAGMRT
                     TLGWVLFGVVWSLAVIGIVMKAVARERALVIGTVLYAVMGWLIVVGFRELVTHLPPVS
                     VQLLFAGGLAYTIGILFFAWRRRYMHFVWHLFVLAGSALHFFAVLML"
     gene            complement(3941..4216)
                     /locus_tag="PLY56_08140"
     CDS             complement(3941..4216)
                     /locus_tag="PLY56_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOJ21491.1"
                     /translation="MMPPVKRCTVTECFYNQAGQCRACAINVGGSHPQCDTFMKSSTH
                     AAPCDLGQVGACKVSQCKYNENLSCTAPGIEVSHHLTHADCLTFEPR"
BASE COUNT          765 a         1519 c         1507 g          784 t
ORIGIN      
        1 gagccgtggt actccacctt ctggggcgcc aacgtccgcg gcccggacta tcgggacgac
       61 ccggaagtgc gcaagatcat cctcgagaac gaacgccgcg aaatgcgctt catgaagagc
      121 ctggggctca acagcgtcgc cttcggtgac gacttgcgca aggatctgga gctggcggat
      181 ggcagggttc gcctcaagcc cgacaaccgc ttcgcgctct ggatggatct ctacaaagag
      241 gccggcatgg gcccgatgcc gttctacggc ttccagccgc tcggtgccgg caacctcctg
      301 gcctggctcg agcgcgagaa gctgaaggag cggtttacgc cccaatggaa caccgcctac
      361 cggagtttcg tggaggagtg ccagcgcatc ggcaaggagc gcggctggcc cgagatcctg
      421 tggtacatct cggacgagtt gagcaaccac ggtgaagagg gcgccaagct gggcgtggag
      481 ctggcgaagg cactcaaggg aatgccaggc gtgcgcacca tcgcctcgat gaacgggccc
      541 tgggagcaca tcatggtgcc ccacctcaac atctccatgc ccaacatcgc cttcccgatc
      601 accgaggaga cggtgaagaa ggtgcgcgac tccggctccg agctgtggct ctacaactgc
      661 ggcgacgagc gcctcaacct ggggctctac ccctggcgcg tgaaggctgg cgggcgcttc
      721 cagtggcact accgctacat caatgccgat ccttgggatg atctggacgg cgccggcgcc
      781 gacacccaat actgcctctc gctgcccgga cccgatggcc cggtccccgc ggtgaagtcg
      841 cagatggtgc gcgaagccgt ggatgaccac cgctacatcg ccaccctcga gcaggcgatc
      901 gcccgggcgc gctccgacgc gaagaagcag gcggccgtcg ccaaagcgac gcgattcctg
      961 gaggagctgc gcacgcgcat cccggtcgat ttccgcacgc tcgtgggcta ccaggtcgat
     1021 ccccgagcag ccggggcttc ggtcggaggc gagttcaaga acaccgacgc gctggaccgc
     1081 gtgcgctggg ccgtggccca gctgatcctc gagctgcaga cggccggcgg gggtgcgacc
     1141 accgggaagg gaaagtgatg aacgctccag cacatggccg catcgcggcc ctttcccgct
     1201 ggcgggctct cctttttggg gggctgctct tgctggcgcc ggctgtccat cagccggccc
     1261 aggcggaagc ggctctgccc cggatagccg tcgcacgcgc aagctccccc gtggtgatcg
     1321 atggccggct ggacgacccg gcctggaata ccgccactcc gcactcgaac ttccgcgcgc
     1381 ttgccgcccg cgggcgcgaa acggcgcctc cggaggagac catcttccgc gtgcttttcg
     1441 atggcgaggc actcttcctt ggcattgtct gccgcgagcc tcagataggg aacctggtcg
     1501 ccaatgccga gggcccggat ggccgcatct ggctggatga ctcggtggag gtgcttctcc
     1561 agccgaccgg acgcttctat taccagttcg ggatcaacag ccgcggcacg cgcttcgatg
     1621 cccgtatgcc ggtcgcggac gatatcagcg agaaagaggt ggccggcggc gccctctggg
     1681 atggcgtgtg ggagtcggcg gtcagcgaag gcgacggcga atggcgcatc gagctgcgca
     1741 tccccctcgc cacgctggac ctgggtccgg agtctctccc tgcctggcgc ctgaatatcg
     1801 ggcgcacggc agcccggcgc atggagtata gcgcctgggc gcccgtcgaa aagggcttcc
     1861 acgacctgcc tcgcttcgga tacctggagg ggattgtcat cgatagcgcg cgcttccccc
     1921 tggatggctc cgcgctcgcc ttcccacccc tcctgctcgg ccggaacgag atccagctct
     1981 cgcttccggc gcggcagccg gggcggttcc gtgccctcac gtcgttgcgg gaatggagcc
     2041 ccgacggaag cgcaacgcgc cagaccgcgg gcgaagcgcc agtcaccggt ggcaagtggt
     2101 cgctggcatt cccggtgccg gtgacccgcg ctggcgttct gtatgaactg ggcctggaga
     2161 tccaggacga ggtcaccaac acccccgtgc tgttgcgcaa gcacgtcttt cgggcgccgg
     2221 acccgttcga cgcgtcgctc gattggatga tctcctacgg gtcggaccgg accgcgcgcg
     2281 ctacgatgac gctcgcggtg gccgagagtt ccgcgcgcgg caaggtggaa gtggccctct
     2341 gggatggcgg cgcaaagccc cagcagcgga tcacccggaa cgtgagcaag ccgggcacgc
     2401 tgcacgtggc gctcccgctc gcgaagctcc cggatggctt ctaccgcgtg gaggtcagcg
     2461 ccacgctcgc cgacatcggc cgcttgagca aggaactccg tttctacaag acatccggcc
     2521 ctttcgatca gtaagggcgc acggcgaggc agggatcgcg cggctgggga gagaaccagc
     2581 agagcatgag cacgctgttg gtagccgcgc ctctcctcgc cgcgccggcc ggggagaggc
     2641 cactgtcccc ctacttcagc gcgcgtgaat tgtcggcgcg atgggcccgc tgcgaggcgg
     2701 atgcgatgcc cgggcccaga gtctaccatt ccgccgctac cctcgcgaac cccgcatcgg
     2761 tggctcccgc ccgaactagc cccggccgcg gcaacacgcc cgtggtagcg cgcgggcgcg
     2821 tgctggccag cccacgggag tctctcggcc ccgcccgtca gatcaacggc catagccgct
     2881 atggcggccc aggcgcccgc gctcccatgg aggcggcgcg gcatctgccg acgcggcggt
     2941 ggcgccggtg cggataagag aaacgccccc ggggcttgcc cgaatgttcc gacccgccgg
     3001 ctccagccga tagagccgca gatcacaaag ggcacctatt tgaaaaaccc cgcattcagg
     3061 cgcacgcatc agcaaccgtt ccgccatttt cgcacagaag gaggagtggg gtactgcgat
     3121 ggcatctccg gatgaggtga aaagagaaga gctgtggaat agcatcagcc acggcatcgg
     3181 catccccctg gggatcgccg cgctcgtcgt ggccgttgcc ttcgcctcgc tggagcggga
     3241 tatctggaag atcgtctcgg tgagcatcta cggcacgacg ctggtactca tgtacaccgc
     3301 ctcaacggtg taccacgcga tctcgggtaa ggcgaagcgg gtgtggaacc tgctggatca
     3361 ttccagcatc ttcctgctca tcgccggcac ctatacgccc gtgatcctcg ccggaatgcg
     3421 caccacgctg gggtgggtcc tcttcggggt tgtgtggagc ctggccgtga tcgggattgt
     3481 gatgaaagcc gtcgctcgcg agcgcgctct ggtcatcggc accgtgctct atgccgtcat
     3541 ggggtggttg atcgtggtcg ggttccggga gctcgtcacc cacctaccgc cggtcagcgt
     3601 gcagctcctt ttcgctgggg gcctcgccta taccatcggc atcctcttct ttgcgtggcg
     3661 ccggcgctat atgcacttcg tctggcacct cttcgtgctg gcgggaagcg cgctccactt
     3721 cttcgcggtg ttgatgctct gaggcgccgg ctggggcggc ccggcgtcag gggaacgagg
     3781 atacgcgggg ttgccgatgc aggcaggttt tcgcctgaag ccaggcatcc tccatcctct
     3841 cactggccac gggcccctgc ccaggcacgg gagggcgttc gtagtggccg gttcggaccg
     3901 gccactacga agctgccgtc tccccctgaa gccgcatccg ttagcgcggc tcgaaggtga
     3961 ggcaatcagc gtgcgtcagg tggtgcgaga cctcgatccc cggagcagtg catgagagat
     4021 tctcgttata cttgcactgc gagaccttac acgcgcccac ctggcccaga tcgcacggcg
     4081 cagcatgcgt cgaggacttc atgaacgtgt cgcactgcgg gtgagagccg cccacgttga
     4141 ttgcacacgc gcggcactgc cccgcctggt tgtagaagca ctctgtgaca gtacagcgtt
     4201 tcactggtgg catcatcggc ttcctccttc tggcgagggt ctctctccat gttgacgata
     4261 cagctcgtca cgggtgaccc cacgcctcgg gtagcgattc gtgcgcagtt gagcggtcag
     4321 aaacggaacg cagtcggcag ccttgcatcc gtttcgtcgc ggattccgca aaccgcctct
     4381 tgagttgacc acatcctcta gcgctcagga gcaaaagctg tgccgagagc cgtcgggcct
     4441 cagcgcggct cgtaggtgat gcaatcagca tgcgccagat gatgcgagac gtcgatcccc
     4501 ggagcagtgc acgagagatc gtggttgaac ttgcactgcg agaccttgca cgcgcccaca
     4561 ctgcccctat cggaa
//
LOCUS       DAOSCV010000282         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11987_length_4559_cov_3.508215, whole genome shotgun sequence.
ACCESSION   DAOSCV010000282 DAOSCV010000000
VERSION     DAOSCV010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11987_length_4559_cov_3.508215"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLY56_08145"
     CDS             <1..531
                     /locus_tag="PLY56_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21492.1"
                     /translation="SYPDLVECARRSFPGHRVIWATTAQGARLLAANSVGVEVLELCE
                     GGFSALALRSAVARMRAAAPSACLIAYENPRRTGNLMLELLALSSGARRLYMLADGSA
                     CRPVGRGRLALRVLAGMGVAVALSAAALVMGGLLACMLPLAAFLYRSFGPRGCVDEHG
                     SSSIGPGERGVRPLRG"
     gene            470..1465
                     /locus_tag="PLY56_08150"
     CDS             470..1465
                     /locus_tag="PLY56_08150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ21493.1"
                     /translation="MNMEAAASALESVGCALCGDSETDALAKIRDLKFFTPGEFCLVR
                     CRRCRLIYLNPRPSPAGIAAYYPKEYWSESVGELPDVYLDRATRHMVNAICRRYPGGR
                     VLDVGCGTGGLVAYFCDRGMDAMGVEPMARPAQLAREAGLNVVTGYLAEAAFPDDSFD
                     VITLVDVLEHTHDPVAVLAEARRVLKPGGMLMAKVPNISSLQARLMGPWWFALDVPRH
                     LYHFSPATLRSALSRAGFRSVFARALPGVSGGLIFELSTLYWLRGLLLARRGITVPPV
                     DERPASESLDGQVYASVPRAGKRAFRWCLRHLAYAPLSIENAIGRSVAIVGTGMK"
     gene            complement(1480..2418)
                     /locus_tag="PLY56_08155"
     CDS             complement(1480..2418)
                     /locus_tag="PLY56_08155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ21494.1"
                     /translation="MTHDIQGQERPSPEKPHDPSEGGLLPISVCIIAHNEEAVIRGCV
                     ESVIGHAAEILVLDAGSTDQTAAILQELQSECPRITLFRGENDPNLNANKMRMIHRAT
                     QPWVLSLDADERIPEALWNEIATAITREDVNGFWIPRKNYFVGRWLRYGDFYPDPQRR
                     LFRRGRGGFAMRHVHEWLDVEGESATLSQPFIHYAFPNVQTFVRKSCFYAQFVAEIWA
                     DRGVRWSLINHLRYGIWGPSRAFFRRYVRHQGFRDGFPGFLAAFILGFQDLLSYATVK
                     ELTTPPNDTIRPHARFGVSRETHGNPDLPDPADEGP"
     gene            complement(2440..4179)
                     /locus_tag="PLY56_08160"
     CDS             complement(2440..4179)
                     /locus_tag="PLY56_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21495.1"
                     /translation="MDSLMARAARLLILAALYTVSFVVMTYPLARDAGNAFVCAPATG
                     STDAYVFLWNIDQFTRSIRSGGPYFWTDWVVHPIGINLLFHTFCPLYGLAGLIIPDPV
                     RALNTMVFLSFVASGLGGYALCRLFCRSRALCAVAGFAYAYSPYKLARLEGHYNLLLT
                     GAIPFFIRFVIRGLAYDETRKRWRVASTRSLAGAGLCLALTILSDHYYTFYLLLFLTL
                     YLVWPPARHSRLARWAKQQPWTAAAICVIVPTLAVLIRKVLQLQIAMHGQMALTLDVL
                     GLLLPPRNGLLFRGQVPASWYHPHDHVAIHGFYIGYAILLPAAWFLLRRRFAAGPPAL
                     AALVFVTAGFALLAMPVVRIHHHPVVALPSVFFYFLPGVGDFREAARAISILMLFLPL
                     FAALAVEERAQRLSAAARLAIAPAWLVLLALEYFQSPLPIVNVRNVPAVYRELARREE
                     GVLLEVPFWLSCGYGSIGDPQVQQQLFQITHQKKLVSGIASRMPQRVFDWYLADPVLR
                     ELTHLEQHPEERPSHPTTNEVDACIRRCSIRYVLVPAEYRATAAGRYAEGAFKAKAVR
                     REEIDGALLLTLY"
     gene            complement(4289..>4559)
                     /locus_tag="PLY56_08165"
     CDS             complement(4289..>4559)
                     /locus_tag="PLY56_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21496.1"
                     /translation="VALAQSAHRFLFTWKNIHDPAWTASHWALLVPHCIESVRRGQVE
                     FARSLVAALPRLPEALRARLQARRQARVSDREVLRLADPGGLQMG"
BASE COUNT          777 a         1374 c         1629 g          779 t
ORIGIN      
        1 agttacccgg atctggtcga gtgcgcgcga cgttccttcc ctgggcatcg cgtgatctgg
       61 gcgacaacgg ctcaaggagc gcggcttctc gcggcgaata gcgtgggcgt ggaggtgctg
      121 gagctctgcg agggaggttt ctccgcgctg gcgcttcgga gcgcggtggc gcgcatgcgc
      181 gcggcggcgc catcagcgtg tctgattgct tacgagaacc cccggcgcac cggcaacctg
      241 atgctggagc tcctcgcgct ctccagcggt gctcgccgac tgtacatgct ggcggatggc
      301 tctgcatgcc gccccgtggg gcgcggccgg ctcgctttgc gggtactggc cggtatgggg
      361 gtggcggttg ccctcagcgc ggctgccctg gtgatgggtg ggcttctcgc gtgcatgctg
      421 ccactggcgg ctttcctcta tcgttcgttt ggacccaggg ggtgcgtaga tgaacatgga
      481 agcagcagca tcggccctgg agagcgtggg gtgcgccctc tgcggggata gcgaaacgga
      541 cgcgctggcc aagattcgcg acctcaagtt cttcactccc ggcgagtttt gcctggtgcg
      601 gtgccggcgg tgccgcctga tctatctcaa cccgcgcccc tccccggcgg ggatcgcggc
      661 ctactacccc aaagaatact ggagcgagtc ggttggcgag cttccggacg tctatctgga
      721 tagggccacc cgacacatgg tgaacgcgat ctgtcggcgc taccccgggg gccgtgtcct
      781 cgatgtcggg tgcggcacgg gcggcctggt ggcctacttc tgcgaccgcg ggatggatgc
      841 gatgggtgtg gagccgatgg cgcgtccggc gcagctcgcg cgcgaggccg gcctcaacgt
      901 ggtcaccggg tatctggcgg aagcggcctt ccccgatgac tccttcgatg tgatcaccct
      961 ggtggacgtg ctggagcaca cgcatgaccc ggtcgccgtg ctggcggagg cgcggcgcgt
     1021 gctgaagccg gggggaatgc tgatggccaa ggttccgaac atttcttcgc tgcaggcgcg
     1081 cctcatgggc ccgtggtggt ttgcactgga tgtgccgcgg cacctctacc atttctcgcc
     1141 cgccacgctg cggagcgcgc tctcgcgcgc cgggttccgg tctgtcttcg cgcgggctct
     1201 gccgggagtg tcgggcggcc tcatctttga gctgagcacg ctctactggc tgcggggcct
     1261 ccttttggcc cggcgaggca tcaccgtgcc gccggtcgat gagaggccgg cgagcgagtc
     1321 tctcgatggc caggtttacg cttcggtgcc tcgggcagga aagcgggcgt tccgctggtg
     1381 tctccggcac ctggcttacg cgccgctttc aatcgagaac gccatcgggc gctctgtcgc
     1441 catcgtgggc accgggatga aatgacaggc gtgcggcgac tacggtccct catccgctgg
     1501 atcgggcagg tccgggttgc cgtgcgtctc tcttgagact ccgaagcgcg cgtggggccg
     1561 gatcgtgtcg ttgggcggcg tcgtcagctc cttcacggtg gcgtaggaga ggaggtcctg
     1621 gaagccgagg atgaaggcag ccaggaagcc ggggaagcca tcgcggaagc cctggtggcg
     1681 cacgtagcgc ctgaaaaagg cgcgcgaggg cccccagatg ccgtagcgga ggtggttgat
     1741 gagcgaccag cgcacgcctc tatccgccca gatctccgcg acaaactggg catagaagca
     1801 gctcttgcga acgaaggtct gcacgttggg gaacgcatag tggatgaaag gttgggagag
     1861 cgtagccgac tcgccctcga catccagcca ctcgtggacg tggcgcatcg cgaacccgcc
     1921 ccggccccgg cggaagagcc ggcgctgcgg gtcggggtag aagtcgccgt agcgtagcca
     1981 tcggccaacg aagtagtttt tcctcggaat ccagaagccg ttcacatcct cgcgcgtgat
     2041 ggcggtggca atctcgttcc acagcgcctc ggggatgcgt tcgtcggcat cgagtgagag
     2101 cacccacggt tgggtggccc ggtggatcat gcgcatcttg ttggcgttca gattggggtc
     2161 gttctcgcca cggaagaggg tgatcctggg gcactcactc tggagctcct gtagaatggc
     2221 ggcagtctga tcggtgctcc cggcatccag gacgaggatc tcggccgcgt gtccgatgac
     2281 gctttcgacg cagcctcgga tcacggcttc ctcgttgtgc gcaatgatgc aaacggagat
     2341 cggcagcagc ccgccttccg aggggtcgtg cggtttctcc ggcgacggtc tctcttgccc
     2401 ctggatgtca tgtgtcatgc aaatcctccg gtgcgcggct caatacaggg tgaggagcag
     2461 cgcgccatcg atctcctcgc gccggaccgc ctttgccttg aatgcacctt cagcgtagcg
     2521 accggccgcg gtggcccgat actccgccgg caccagcacg tagcggatag agcaccggcg
     2581 aatgcacgcg tccacctcgt tggtggtggg atgcgatggc ctttcttcag gatgctgttc
     2641 caggtgcgtg agctcccgga ggaccgggtc tgccaggtac caatcgaaga cgcgctgcgg
     2701 cattcgcgat gcgatgcctg acacgagttt cttctgatgg gtgatctgaa acagctgctg
     2761 ctgaacttga gggtcgccga tgctcccgta gccgcacgag agccagaagg gcacctccag
     2821 gagaacgccc tcctcgcgtc ttgccagctc acgatacacg gctggcacgt tgcgcacgtt
     2881 cactatcggc agcggcgact gaaaatactc cagggcgaga agcacgagcc acgcgggcgc
     2941 tatcgcgagg cgcgccgctg ccgaaagtct ctgagccctt tcctcgacgg ctagcgccgc
     3001 gaagaggggc aggaagagca tgaggatcga gatggcgcgc gctgcctcgc ggaagtcgcc
     3061 gacacccggc aggaagtaga agaaaaccga gggcaaagcc accacggggt gatggtgaat
     3121 gcgcacgacc ggcatcgcca ggagggcgaa accggcggtg acaaagacca gcgcagcgag
     3181 ggcgggcggc cccgcggcaa agcgccggcg caataggaac cacgctgcgg ggagaaggat
     3241 ggcgtagccg atatagaagc catggatggc gacgtggtcg tgcgggtggt accacgatgc
     3301 cgggacctgg ccacgaaaca ggaggccgtt ccggggtggc aagagcaatc cgagcacgtc
     3361 gagggtgagc gccatctgcc cgtgcatggc gatctgtagc tggagtactt tgcggatgag
     3421 caccgccagg gtgggcacga tcacgcagat cgcggcggcc gtccagggct gctgtttcgc
     3481 ccatcgcgcc agcctggagt ggcgggccgg cggccacacg aggtagaggg tcaggaagag
     3541 gagcaggtag aaggtgtagt agtggtcgga gaggatcgtg agggcaaggc atagccctgc
     3601 cccggcgagc gaacgtgtgc tggccactcg ccaccgcttg cgcgtctcgt cgtaggcgag
     3661 cccgcgtatc acgaaccgga tgaagaaggg gatcgccccg gtgaggagca ggttgtaatg
     3721 tccctccagc cgcgcgagct tgtagggcga gtaagcgtag gcgaaaccgg cgacggcgca
     3781 cagcgcccgg ctacggcaaa agaggcggca aagcgcgtag ccccccagcc cggaagccac
     3841 gaaactgagg aacaccatgg tgttgagggc acgcaccggg tctgggataa ttaggcccgc
     3901 cagcccatag agggggcaga aggtgtggaa gaggaggttg atcccaatgg ggtggaccac
     3961 ccagtctgtc cagaagtaag gaccgccgga acggatgctc cgcgtgaatt gatcgatgtt
     4021 ccagaggaag acgtaggcgt ctgtggatcc ggtggccggt gcgcacacaa acgcgttccc
     4081 cgcatcgcgc gccagcggat aggtcatcac cacgaacgac accgtataga gagcggccag
     4141 gatgagcagt ctggccgcgc gggccatcag ggagtccatg ggagcgctgc cgggcctcct
     4201 gagacgggtt gtcggccaag cccatgagtg caagcggatt ctccacccgg gccggccagg
     4261 cacattatac gggggggatc ccgcgttgtc aacccatctg gagcccgccc ggatctgcca
     4321 ggcggagtac ctcgcggtcg gagacccgcg cctggcgccg ggcctggagg cgcgcccgta
     4381 gcgcttctgg caggcgggga agtgccgcca ctaggctgcg cgcgaactcc acctgccccc
     4441 tgcgtaccga ttcgatgcag tggggcacca ggagtgccca atggctcgct gtccacgccg
     4501 ggtcgtggat gtttttccac gtgaagagga agcggtgggc gctctgggcg agggcgacg
//
LOCUS       DAOSCV010000283         4556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_11997_length_4556_cov_3.126639, whole genome shotgun sequence.
ACCESSION   DAOSCV010000283 DAOSCV010000000
VERSION     DAOSCV010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11997_length_4556_cov_3.126639"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3161)
                     /locus_tag="PLY56_08170"
     CDS             complement(<1..3161)
                     /locus_tag="PLY56_08170"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOJ21497.1"
                     /translation="MRLPWIFLLLLAALPPTYGQTPASLIANGNFAQRSPNRTTPDGW
                     NPFAHEGKYQVSVDPTGGRDGSPCVKVEGAAESANGRAGVLSRTETFPAPRGLRASVL
                     TRGTSGRRAIVVQFFDAANPKEIVTVQSATVPGEDAEWREFTAEFEIPRALRRKPIQV
                     IVLLYLYGTGSCAWDDLSLAPVTQVELKVRDEETSPYLIRPNPPDGAVVAQNPPDFRW
                     RPEKNAETYTLQLCRDPGFTGPSLITEEGIPLNCYNLSRPLEPGRWYWRIRFTDDEGQ
                     HSSFGRARSFEVPANAVQFVLPPVEEVLQKHLRRDHPRLYVTREELPALRARRQAEGK
                     AWWEQYEPLLERLLKAEPAAEPVDCDLRGRPIDLEYFKIDSRLRAAAGQVSSGLWNLA
                     FGYLITGEPRYAEGAKRYLLHLSRWDPDGATGFRSNDQVFRDLLIKSAMAYDWIADTL
                     SPEERAPIQKAIADRCRIMYRIYATDNKILNYPLDSHGQSNIGYLGLATLAIAGEVPE
                     ADEWTAYTIRVYSANFPPWGGDDGGWSQGVSYWKWSCSFALQYLDALKTATGIDLYQK
                     PWFRNNGLFKLYFQPPWCGFSYFGDTGPYGVDATDKRNLERYAAVHRNPYYQWYADLV
                     SAPPDSGPYGFLFPRANVPLRPPVDLPQARVSNDIGWVAMYSHLYAPDGVMLLAKASP
                     YGSFNHSHADQNSFVLAAFGEPLAIDSGYYPWYHSPHHKEWAIRTKAHNTILVDGEGQ
                     KEMEITARGQSVAFHAGKSYAYWCGEAAEAYAGRLNRFRRHILHLRPDAFVIYDDLES
                     PQPATFQWLLHAAERMEIDPARQSVLVRRGQARLRVQHLLPAGLNQSQDDDFGVPPEA
                     RGGSMPNQWHYRAETREKAASQAFLTLLQAFREATPPAQTGRLVRGEGATGLVLGSEV
                     IAFATPGAPRTRALGSLRFDGAVAASRQGAGTARYLLVSGTRLQHGATTLIASSAPCR
                     VEAGWVGGSFESAIATDADANIRLHVGKRPTGVRLDGKTLAPGKWTYDAKSGTIRLRV
                     AKGEHALSVVPAGVPQPAPAGAL"
     gene            3461..4342
                     /locus_tag="PLY56_08175"
     CDS             3461..4342
                     /locus_tag="PLY56_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21498.1"
                     /translation="MLKLGIVGLDGHGPVFANVVNGPEPKVEGARVVAAMPVPSVMIP
                     EEKLQENIEKTRALGIDILEDPKELASRVDGVLVLHDDGSKHQELASLFAPFGKPIFV
                     DKPFETTPEKARALVEACRRHSTPVFSASSLRFSKELTAALADNAGGEIVSAMTYSPF
                     SPKPTMPGWIYYAVHSVEPLFQILGRGCQRVCCLPGEYGPVAIGEWSGGRTGIAKATA
                     RGHWSYGFTLWRQKADQMALVDPAHIYPALLREVKAFIETGQSPVDPEQSVEVVAFMA
                     AANESMANGGKPVALNC"
BASE COUNT          717 a         1469 c         1621 g          749 t
ORIGIN      
        1 cagagcgcgc ccgcgggcgc cggctggggt accccggcag gaacgacgct tagagcgtgc
       61 tcgcccttcg cgacccgcag ccggatcgtg ccggacttcg cgtcgtacgt ccacttgcct
      121 ggggcgagcg tcttgccatc caggcgcacg cctgtcgggc gcttccccac gtgcagccgg
      181 atgttggcat cggcatccgt ggcgatcgcg ctctcgaagc tgcctccaac ccacccggcc
      241 tccacgcggc agggtgccga ggaggcgata agcgtggtgg caccgtgttg caggcgcgtg
      301 ccggagacga gcaggtagcg cgcggtgccc gcgccctgcc ggctggcggc gacggcgcca
      361 tcaaagcgca ggctgcccag cgcgcgcgtg cgaggcgccc ccggagtggc gaaagcgatc
      421 acttccgaac cgagcaccag cccggtggct ccttcgccgc gcaccagccg gcccgtctga
      481 gctgggggcg tggcctcgcg aaacgcctgg aggagggtga ggaacgcctg gctcgccgcc
      541 ttctcccgcg tctcggcgcg gtagtgccac tggttgggca tcgatccgcc gcgcgcttcc
      601 ggaggcacgc cgaagtcgtc atcttgcgat tgattcagcc cggctggcag gaggtgctgc
      661 acgcgcagcc gcgcctgccc gcggcgcacg agaacgctct ggcgcgcggg atcaatctcc
      721 atccgttccg ccgcgtggag cagccactgg aaggtggctg gctgcgggct ctccaggtcg
      781 tcataaatga cgaacgcatc cgggcgcagg tggaggatat gccggcggaa gcgattcagc
      841 cggcccgcgt acgcctcggc ggcctcgccg caccagtagg cgtagctctt gccggcgtgg
      901 aacgccacgc tctgcccccg ggcggtgatc tccatctctt tctggccctc gccatccacg
      961 aggatcgtgt tgtgcgcttt ggtacggatc gcccactcct tgtggtgcgg gctatggtac
     1021 caggggtagt agccactatc aatagcgagg ggctctccaa acgcggccag gacgaagctg
     1081 ttctggtcgg catggctgtg gttgaacgag ccataggggc ttgccttcgc caggagcatc
     1141 actccgtctg gcgcgtagag atgcgagtac atcgccaccc agccgatgtc gttggagacg
     1201 cgcgcctgcg ggaggtccac cggcgggcgc agcggcacgt tggctcgggg gaagaggaag
     1261 ccatagggcc cgctatccgg cggcgcgctc accaagtcgg cgtaccactg gtaatagggg
     1321 ttccggtgga cggccgcgta gcgctccagg ttgcgcttgt cggtcgcatc cacgccatag
     1381 ggtcccgtat cgccgaagta ggagaagcca caccacggcg gctggaagta gagcttgaac
     1441 aggccgttgt tgcggaacca cggcttctgg tagagatcga ttccggtggc cgtcttcagc
     1501 gcgtccaggt actggagcgc gaaggagcac gaccacttcc agtagctgac gccctgcgac
     1561 catccgccgt catcgccgcc ccagggtggg aagttggcgg agtagacccg gatggtgtag
     1621 gccgtccact cgtctgcctc gggcacttcg ccggcgatgg cgagggtggc cagacccagg
     1681 tagccgatgt tcgactgccc gtgggaatcg agcgggtagt tcaggatctt gttgtccgtt
     1741 gcgtagatcc ggtacatgat ccggcaccgg tcggcaatcg ccttctggat cggcgcgcgc
     1801 tcctcgggcg agagagtgtc cgcgatccag tcgtaggcca tagcgctttt gatgaggaga
     1861 tcgcggaaaa cctggtcgtt cgagcggaag ccggtcgcgc cgtcgggatc ccaccgtgag
     1921 agatggagga ggtagcgctt cgcaccttcg gcatagcggg gctcgcccgt gatcagatag
     1981 ccgaaggcga ggttccagag gccggacgac acctgcccgg cggccgcgcg gagccgggag
     2041 tcgatcttga aatactccag gtcaatcggg cgcccgcgca ggtcgcaatc gaccggctcg
     2101 gcggcgggct cggccttcag tagccgctcg agcaacggct cgtactgctc ccaccacgcc
     2161 ttgccctcgg cctggcggcg cgcgcggagc gcgggaagct cctcgcgcgt gacgtagagc
     2221 cgggggtggt cacggcgcag gtgtttctgc agcacttcct ccaccggcgg gaggacgaac
     2281 tgaacggcgt tcgctggcac ctcgaacgag cgcgcccggc caaagctgga gtgctggcct
     2341 tcgtcatcgg taaagcgtat ccgccagtac caccgcccgg gctccagggg gcgcgacagg
     2401 ttgtagcagt tgagggggat gccctcctcg gtgatcaggc tcggcccggt gaaacccggg
     2461 tcgcggcaga gctgaagcgt gtacgtctcg gcgttcttct cggggcgcca gcggaagtcg
     2521 ggcgggtttt gggcgacgac cgcgccatcg ggcgggttgg gccggatcag gtagggcgac
     2581 gtctcctcgt ccctcacttt gagctcaacc tgggtgaccg gcgcgagcga gaggtcatcc
     2641 caggcgcagg agccggttcc atagaggtag aggaggacga tcacctgaat cggcttccgc
     2701 cggagcgccc ggggaatctc aaactcggcg gtgaactcgc gccactccgc gtcttcgccc
     2761 ggcaccgtgg cgctctgcac ggtgacgatc tccttcgggt tggccgcgtc gaagaactgg
     2821 accacgatcg cccggcgccc actggtgccc cgcgtcagga cgctcgcccg gagcccccga
     2881 ggcgcgggga aggtctcggt gcgcgacagg actcccgcac gcccgttggc gctttccgcc
     2941 gcaccctcca ccttcacgca cgggctgcca tcacggcctc ccgtcggatc gaccgacacc
     3001 tggtatttcc cttcgtgagc gaaggggttc cagccatccg gcgttgtccg gttggggctt
     3061 cgctgggcaa agttgccgtt ggcgatgagg ctggccggcg tctgcccata cgtcgggggc
     3121 agcgcggcga gcaggaggag aaatatccat ggcaggcgca tcggggtctc cctttcgctt
     3181 ggcatgcggc ggcagcggca cgtatagcca tttccaccgc actattcgcg ctcatagcgc
     3241 gggcacctgc cagcgccgcg gattccgcat caggcaggta gagaacggca accccgttcg
     3301 cgtgagagcg acagggatct ggccccgctg gcacgaaagc acgagagggc gcgtgcgcag
     3361 aggcatggcg cggggagcga gggtgtgctc agggcacgcc ggctcccgcg gccgttcgtg
     3421 tgcttccggc ggcgctcacg tcattatgag gagaaatcat atgttaaagc tcggaatagt
     3481 cggtctggat ggacatgggc cggttttcgc caacgtggta aatgggccgg agccgaaggt
     3541 ggagggggcg cgcgtcgttg ctgcgatgcc ggtgccttcc gtgatgattc ccgaagagaa
     3601 gctgcaggag aatatcgaga agacgcgggc cctcgggatc gatatcctgg aggacccgaa
     3661 ggagctcgcc tcgcgtgtgg atggcgtgct cgtgttgcac gacgatggct cgaagcacca
     3721 ggagctggcg agcctcttcg cgccgttcgg caagccgatc tttgtcgaca agccgttcga
     3781 gacgacgcca gagaaggcgc gcgctctcgt cgaggcgtgc cggcgccact ccacgccggt
     3841 cttctcggcc tcttcgctcc gcttctccaa ggagctgacg gcggcgctcg ccgacaacgc
     3901 gggcggcgag atcgtctcgg cgatgaccta ctcgcccttc tcgccgaagc ccacgatgcc
     3961 cggctggatc tactacgccg tgcatagcgt cgagccgctc ttccagatcc tgggccgggg
     4021 ctgccagcgc gtctgctgcc tgcccggcga gtacggcccc gtggcgattg gcgagtggtc
     4081 gggcgggcgc accgggatcg ccaaggcgac ggcgcgtggc cactggagct acggcttcac
     4141 cctctggcgc cagaaggcag accagatggc cttggtcgat cccgcccaca tctaccccgc
     4201 cctcctgcgc gaggtgaagg cgttcatcga gacgggccag tcgccggtgg atccggagca
     4261 atcggtggag gtggtcgcgt tcatggcggc ggccaatgaa tcgatggcga acggcggcaa
     4321 gccggtcgcc ctgaactgct aacccgcgcc tggcgggcac gcgatccgcg ctgcttgcga
     4381 gcgaagccct tcgagctggg ttgggttgct cccgcgggca atccaaggat ggcggggctg
     4441 ggaagggaag ccccgcgagg ggctttgccg ggaagcagcg cggagcttcg agcaccgcgc
     4501 gcgggccgag caggccccgc gccggtgggg aaccagggcg cggttgggaa cacaga
//
LOCUS       DAOSCV010000284         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12019_length_4548_cov_3.855108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000284 DAOSCV010000000
VERSION     DAOSCV010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12019_length_4548_cov_3.855108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLY56_08180"
     CDS             complement(<1..748)
                     /locus_tag="PLY56_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ21499.1"
                     /translation="MEHSPPHGGGSNLALAIEALESGQTEQAIQLLQFATSMNPNSGE
                     AHFHLGRAYAACDNLTLAETSLRRAVELLPGDAPALCALGKVLARRQSYDEARRVLEA
                     CLDADAQCQEAREALELLPRPEDRAIALPDLDVLPARLELATDTGTRMRRLLLFTASM
                     GILLSILLAFAFLQVQKTLSMHSARTGASHAETSWSARRDGVPTTHEEGLAPDEAGPA
                     VEDAEEALRAAAACLRRLYEAQREYHTHHEH"
     gene            complement(909..1787)
                     /gene="amrB"
                     /locus_tag="PLY56_08185"
     CDS             complement(909..1787)
                     /gene="amrB"
                     /locus_tag="PLY56_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ21500.1"
                     /translation="MAMPAMRAPAVAGLFYPGTADALQQQIQRCFLDAPGPGRLPLAA
                     PEGPRQILGLVVPHAGLVYSGHVAAHAYQHLAEDGLVEVAIILAPNHHGLGADNAVWP
                     EGGWETPLGNMPVAADVAAAILEATPLAHADPMAHLRDHAIEVQLPFLQLLYGAGLSI
                     VPISMKRYELDAAIELGEAIATAMAGRNAVVIASCDFTHHEPASRARTQDERALEMIE
                     HLDPAGLFRQVQTHGITTCGYGPVAAMLAAARRMGAVSAQRLAYHTSGEVTGDYDRVV
                     AYAALKVMRPASGQES"
     gene            complement(1801..3633)
                     /locus_tag="PLY56_08190"
     CDS             complement(1801..3633)
                     /locus_tag="PLY56_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOJ21501.1"
                     /translation="MMLSSIVWTALAALAAISITIRVLRGLRIGQQTPGAALCVACAL
                     LILSAFLARYHAVQKEDAERRFHAGLEARRQRELARADPTKQGTPSPPAVTTPAGILP
                     RPLEPGTHRETPSHRAGPVRIVDYALDLSLFPDRHSLSARAEFTVVARMPIDRIELAL
                     SPEFRVHALHVNGAPVTFLHTNDLVSFPYRLREGKAATLAVRYSRRVSVPLLRAGDLI
                     DPRGIYLRPESRWYPSVGELDFHAPLRLSARVPRGMTVVSSGQLVRKSVEASTVTFHW
                     ESIVPVRMICLAAGRYAHSRNADGPVAIETFLHPNHASRARAYQETVQRIVRFYASRF
                     GAYPYPKLTIAEIPVFPGGYGSTALLLLTEKSFAEKEIPTHFLAHEIAHQWWGNHVAP
                     QGLGAGWLSEAFAEYSAVLYQEHAGGPKALQEALAGLEARYQTRLREGTEEAIAETDP
                     YDQSGAYEGTVYYKGAYVLHSLRCLIGEEQFRRLLRLFAERYGAGTARIADFEALAGE
                     VHGKPLTWFFDQWLRRPGAPRLRVAVAKEGPPAVLTVVQEGAPYRGEMQLLVTDGAGK
                     TRHTLHLDGSLTRVKIPVRGTITRVEIDPDRAWLLHTPQRVHSL"
     gene            complement(3696..4004)
                     /locus_tag="PLY56_08195"
     CDS             complement(3696..4004)
                     /locus_tag="PLY56_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21502.1"
                     /translation="MAKEELKTRQDAVGTSFGVLIFLTGIGLLAFVFLLALGLFKDPG
                     VLTAMATTPASATPAAEGGSGVTGALIGLGVKVVALFIMALSGSLVTSKGIHLYLASR
                     "
BASE COUNT          771 a         1447 c         1555 g          775 t
ORIGIN      
        1 aatgctcgtg gtgggtgtgg tactcccttt gcgcctcata gagccgccgg aggcaggcgg
       61 ccgcagctct caaggcttcc tcggcgtcct ccaccgcggg acccgcctcg tcaggcgcca
      121 ggccctcctc gtgggtggtg gggaccccat cccgccgcgc gctccacgag gtctccgcgt
      181 gcgatgcgcc ggttctggct gagtgcatgg agagcgtctt ctgcacctgt aggaaggcga
      241 acgccagaag aatcgatagc aggatgccca tgctcgcggt gaacaggagc agccgccgca
      301 tgcgggtgcc ggtgtcggtg gcaagctcca ggcgcgctgg tagcacatcc aggtcgggga
      361 gtgcaatcgc gcggtcttcg ggccggggaa gcaactccaa tgcttcgcgg gcctcctggc
      421 attgcgcatc ggcatcgaga caggcttcga gtaccctccg cgcttcgtcg tagctttgcc
      481 gacgtgccag caccttgccg agggcgcaca acgctggagc atcccctggg agaagctcga
      541 cggcgcgccg gagcgaggtt tcggcgagag tgaggttgtc gcacgccgca taggcgcgcc
      601 cgagatgaaa atgcgcctcg ccgctgttcg ggttcatgga cgtggcgaac tggagcagct
      661 ggatcgcctg ctcggtctgc ccgctttcga gggcttcgat cgcgagggcg aggttgctac
      721 ctcccccatg gggcggtgag tgctccatgc gcgtttctcc ttcaggaaag cggagccaac
      781 agcaaatccc cgccgcgcaa cgcgcgagcg gacctgctgg cttcctgtcg ggctgggcgt
      841 atcactccgg aggagcgccg cgggcacgcg gcctccaccg cgttgctcca ggacttcttg
      901 cccgcccgtc agctttcctg cccgctcgcc gggcgcatca ccttcagggc cgcgtatgcg
      961 accacgcgat catagtcacc ggtcacttcg ccggaggtgt ggtaggccag cctctgcgcc
     1021 gagacggctc ccatccggcg ggcggctgcc agcatcgcgg cgacgggccc atagccgcag
     1081 gtggtgatcc catgcgtctg cacctggcgg aaaaggccgg cagggtcgag gtgctcaatc
     1141 atctcgaggg ccctttcatc ctgggtgcgc gcgcgcgagg ccggctcgtg gtgcgtgaaa
     1201 tcgcacgacg cgatgacgac ggcgttgcgg cccgccatcg ccgtggcaat cgcctctccc
     1261 agctctatcg cggcgtcgag ctcataccgc ttcatggaga tgggcacgat ggatagcccc
     1321 gccccgtaga ggagctgcag gaaagggagc tgcacctcga tcgcgtgatc tcgcaggtgg
     1381 gccatcggat ccgcgtgcgc cagcggggtg gcctccagaa tggccgccgc gacatctgcc
     1441 gcaaccggca tgtttcccag gggtgtctcc catcctccct cgggccatac ggcgttatcg
     1501 gcgcccaggc catgatggtt gggggcgagg atgatggcaa cttctaccag gccatcctct
     1561 gccaggtgct gatacgcgtg tgccgcgacg tgcccggagt agaccaggcc ggcgtgtggg
     1621 acaaccagac cgaggatctg gcggggcccc tcgggggcgg ccagcggcag gcgcccagga
     1681 ccgggagcat ccagaaagca gcgctggatc tgctgctgca gcgcgtcggc agtccctgga
     1741 tagaacaggc cagcgacggc aggcgcgcgc attgcgggca tcgccatctc acaccctcct
     1801 tcacaggctg tggacccgtt gcggcgtatg gagcagccat gcgcggtccg ggtcaatctc
     1861 tacacgggtg atggttcccc gaaccggaat cttcacgcgc gtgagggagc catccagatg
     1921 cagagtatgc cgcgtcttgc cggcgccatc ggtgaccagg agctgcattt cgccacggta
     1981 gggcgcgccc tcctggacga cggtgagcac ggcgggcggg ccctccttcg ctacagcaac
     2041 tctcaggcgc ggcgcgccag gccggcgcag ccactgatcg aaaaaccagg tgaggggctt
     2101 cccatgcact tcgccagcca gggcctcaaa gtcggcgatt cgcgctgtgc cggctccata
     2161 tcgctccgcg aagaggcgga gtagccgccg gaactgctcc tccccgatca ggcagcgcag
     2221 cgagtgaagc acataagccc ctttgtagta gacggtgcct tcgtaagctc ccgactggtc
     2281 gtagggatcc gtttcggcga ttgcctcttc ggtgccttct cgcaggcggg tctggtagcg
     2341 tgcctccaag cccgcgagcg cctcctgtag cgccttgggc ccgccggcat gctcctggta
     2401 gagtacggcg gagtactcgg caaacgcctc gctcagccat cccgccccga ggccctgagg
     2461 cgcgacgtgg tttccccacc actgatgcgc aatctcgtgc gccaggaaat gggtggggat
     2521 ctccttttca gcgaagctct tctcggtgag cagcagcaac gccgtggagc cgtagcctcc
     2581 gggaaagacg gggatctcgg caatcgtcaa tttgggatag ggataggcgc caaaccggct
     2641 ggcgtagaag cgcacgatcc gctggactgt ttcctggtag gcgcgcgccc tggatgcgtg
     2701 attcgggtgc aggaaggtct cgatggccac aggaccatcg gcgttccggc tgtgggcgta
     2761 tcgtccagcc gccaagcaga tcatccgcac cggaacaatg ctctcccagt ggaaggtgac
     2821 cgtagatgcc tcaaccgatt tcctcaccag ttggccggag ctgacgacgg tcattccccg
     2881 tggaacacgc gcggagaggc ggagcggcgc gtggaaatcg agctcgccca cggaggggta
     2941 ccaccggctc tccgggcgga ggtagatgcc ccgaggatcg atcaggtctc cggcgcgcag
     3001 gagcggcacc gagacgcgcc gagagtagcg gacggcgagt gtcgcggctt tgccttcgcg
     3061 gaggcggtag gggaacgaga cgagatcgtt ggtgtgcaag aaggtgaccg gggcaccgtt
     3121 cacatgcagc gcgtggacgc ggaactccgg gctgagcgcc agctcaatgc gatcgatggg
     3181 catgcgcgcg accacggtga actcggcgcg cgcgctcaga gaatgtcggt cggggaagag
     3241 ggagagatcc agcgcgtagt cgacgatgcg cacggggccg gcgcggtgag aaggcgtctc
     3301 gcggtgtgtg cccggctcca gagggcgggg caggatgccc gcgggcgtcg tcacggctgg
     3361 tggcgagggc gtcccctgct ttgtcggatc ggcccgcgca agttctcgct ggcgccgcgc
     3421 ctcaagcccg gcatggaagc ggcgctccgc atcctccttc tgaaccgcat ggtagcgggc
     3481 gaggaaggcg gagaggatga gcagcgcgca cgccacgcaa agcgcggcgc ccggggtctg
     3541 ctggccgatc cgcaggcccc gcagcacgcg gatcgtgatg ctgatggccg cgagtgccgc
     3601 cagcgcagtc cagactatgc tggagagcat catcgcggaa ctgccttgcc tggccccagg
     3661 ctacagcggg cggcgctgcg cccagaagcc ccggcctagc gactcgccag gtagaggtgg
     3721 atccccttgc tggtaaccag ggagccggac agtgccataa tgaagagcgc gaccaccttc
     3781 acgcccaggc cgatcaacgc gccggtcacc ccggatccgc cttctgctgc tggtgtggcg
     3841 ctggcaggcg tggtggccat ggcagtcaga acgcctggat ccttgaatag acccaacgct
     3901 agcaggaaga cgaacgccag caggccgatg cctgtgagaa agatgaggac tccaaacgac
     3961 gtcccgacgg catcctggcg ggtcttcaac tcttctttgg ccatgggctt cccctctgaa
     4021 ggcgttgtct gcatgatggc ctattctatc atatgggtac gccagggtca agaaaatgga
     4081 tggtcgggaa cgtcgcaagt agaaggagtc cttaccagaa gcacgaacga ttctcggatg
     4141 aatagtgcac cgcaggccgg atccggagtg ccatcttgag atctgcgcat caagttccag
     4201 gcgtatctct tgagctcgac gccgtgcagg tagctggcag cgcacccatc cgcgggctgt
     4261 tccacagttg gttggggagc cgtgcgggtg acggggcggt gttcctaggg caccgccaag
     4321 ccggagggct gccaccccaa tgcgcgcccc ggggaccgga tggtgccgga tatgcgctcc
     4381 tatgattgcg ctgagcctgt tggcgttttt actcatcgca gggattctgg cgttctcgct
     4441 cctccagtgg gaaggtgcgc agcggcgcga gttactctat ttccatctca ccctcgagaa
     4501 gccaggttcc aactacttcc gatccgctcg cctcgatcgg gagcagta
//
LOCUS       DAOSCV010000285         4545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12026_length_4545_cov_3.711804, whole genome shotgun sequence.
ACCESSION   DAOSCV010000285 DAOSCV010000000
VERSION     DAOSCV010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12026_length_4545_cov_3.711804"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(370..2838)
                     /gene="lon"
                     /locus_tag="PLY56_08200"
     CDS             complement(370..2838)
                     /gene="lon"
                     /locus_tag="PLY56_08200"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ21503.1"
                     /translation="MDVNEGQTPTQTGGEESGVSIPEELSLLPLRDTVLFPVVVVPLA
                     VSRESSVKLIDDAAVSGSRVIGTVAMKSPEIEKPTLDDVYRIGTAAVIHTMLKLPEGV
                     RLIVQGLRRIEILECLQTEPYLRVRVRVLPEDTPEAELPQDAQMELEALRRSVGTLFQ
                     KVVSLSPDLPDELQAIPQNVQEAHVLADLICAHIRLPIQEKQGLLEIIDVRERLRRLA
                     SLLHRDLEILELQTKIQSQVHTELGKMQRDYYLREQLKQIQKELGETDERTAEIEELR
                     AKIEAAQMPEEARKEADRELDRLSKMPPQAAEYTVARTYIDWLVALPWNTSTEDNLDM
                     REVREVLDADHYGIEKVKERILEYLAVRKFNPESHRHPILCFAGPPGVGKTSLGKSIA
                     RALGRKFFRMSLGGIRDEAEIRGHRRTYIGALPGQIIQGIRRAGSNNPLFMLDEIDKV
                     GADFRGDPSSALLEVLDPEQNGSFRDHYLDVPFDLSRVLFITTANVLDTILPPLRDRM
                     EIIELSGYTEQEKIQIARRHLVPKQMGEHGLTPDHIRWEDDGIRAIVLGYTREAGVRN
                     LEREVAAVCRKVTREFAEGRTEPAVIDAEAVRRYLGVPRFEYEEIAERTSQAGVATGL
                     AWTPVGGDVMFIEATAMPGGRSLMLTGQLGDVMKESAQAALSYVRSHATELGIDHNFF
                     RRNDIHVHVPAGAIPKDGPSAGVTMVTAIASLATGRRVRSDVAMTGEITLTGRVLPVG
                     GIKEKVLAAHRAGLKRVILPERNRKDMLEDVPEEVRQSIQIEYANTMRDVLDWVLEPE
                     DAAPAAEETSPRQGQATPERIAAP"
     gene            complement(2928..3407)
                     /locus_tag="PLY56_08205"
     CDS             complement(2928..3407)
                     /locus_tag="PLY56_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ21504.1"
                     /translation="MSFTRYEDVIRQMEKEMERLTEGWRGFFDPMPGRFWQPRTDVYE
                     TERNLVVKVEAAGLRVDPDTGSLPDVRVDLSPDGRVLTIRGQRVEDCGECVGRVRWYR
                     FEIYFGPFEISIPLPGGVAVEREKITGSYKDGFLIVILPKVARAERSVVHTIPVTGD"
     gene            complement(3786..>4545)
                     /locus_tag="PLY56_08210"
     CDS             complement(3786..>4545)
                     /locus_tag="PLY56_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21505.1"
                     /translation="EKQMATTREEARAMIDAAEKAGKNLMIGHNQRLMPPHVCAKQIL
                     ESGKLGKVISFRTAFAHPGPEFWSVDNHAGTWFFKKGIASLGVTGDLGVHKADLMRWL
                     LGDEFSEVSAVITTLHKRYPDGSLIDIDDNAVLTLRTQGGILGTLMVSWTNYGAEDNW
                     TVLYCENGVLSIGTDPVWNVIVNYRNGDVEQHKVGEISTNVKQVSSGVLDQFVAEILA
                     GKKPAIDGEEGYRALNVILTAMEAAAANKTMKIA"
BASE COUNT          788 a         1575 c         1331 g          851 t
ORIGIN      
        1 cacctgctcg tcttccagga gcgcttcctc cacccgctca accagatccg cctcggcccc
       61 gccgcctttc tggtagccca ttctcctgct cctgctccta cgatctccca tgtacggctc
      121 gcggacccgc cggcgcgccc tggcgaggcg ccccttccac cagacgggta ctgatgacac
      181 tactgctatg gacgcaccca tcccgcttct ggttcgcaac agatgcacgc tcctcgatgg
      241 tgttacccga accgttttcc ggcgaaacag gcacggcggc ttagccacca gacatgcaaa
      301 caggccgctg cccatcgagg ggagcggcct gcgcaaatac actgcgctct cacgcgagag
      361 cgacaggcgt cagggggcgg cgatccgctc gggagtagcc tgcccctgtc gcgggctggt
      421 ctcctcagcg gcgggcgcgg catcctccgg ctcgagaacc caatcgagta cgtcacgcat
      481 cgtattcgcg tactcgatct gaatgctctg gcgcacctcc tccggcacgt cctccagcat
      541 atccttgcgg ttgcgctccg gaagaataac ccgcttcagg ccagcacggt gtgccgcgag
      601 caccttctcc ttgatgccac ccacggggag aacacggccg gtgagcgtga tctcgccggt
      661 catcgccaca tcgctgcgaa cgcgccggcc agtggcgagc gaggcaatgg cggtcaccat
      721 tgtgacgccc gcggaaggtc catccttcgg gatcgccccc gccggcacgt gaacgtggat
      781 atcgttgcgc cggaagaagt tgtgatcgat ccccagttcg gtcgcgtgcg agcgcacgta
      841 gctgagcgcc gcctgggccg actccttcat cacatcgccc aactgcccgg tcagcatcag
      901 cgagcgcccg ccaggcatgg cagtcgcctc gatgaacatt acatcgccac cgaccggcgt
      961 ccacgccagc cctgtggcga cgcccgcctg cgaggtgcgc tcggctatct cctcatactc
     1021 gaagcgcggc acgcccagat agcggcgcac cgcctcggca tcgatcaccg ccggctcggt
     1081 gcggccctcg gcaaactcgc gtgtcacctt gcggcagacg gcagcgacct cgcgctccag
     1141 gttacgcacg cccgcctcac gcgtgtagcc caggacgata gcccggatcc cgtcgtcctc
     1201 ccaacggata tgatcggggg tcaggccgtg ctcgcccatc tgcttcggca cgaggtgcct
     1261 gcgcgcaatc tgaatcttct cctgctcggt gtagccggag agctcgatga tctccatgcg
     1321 gtcgcgcaga ggtggcagaa tggtatccag cacgttggcc gtggtgatga acaacacccg
     1381 cgagagatcg aacggcacat ccaggtagtg atcgcggaag ctgccattct gctccggatc
     1441 gagcacctcc aggagcgccg aggaggggtc gccacggaaa tcggcaccca ccttatcgat
     1501 ctcatcgagc atgaagaggg ggttattcga tccggcgcgt cggatgccct ggatgatctg
     1561 gcccggaagc gcgccaatat aggtgcgccg gtgcccgcgg atctctgcct cgtcgcgaat
     1621 gccacccaac gacatgcgga agaacttgcg gccgagcgca cgcgcgatcg atttgccgag
     1681 cgaggtcttg cccacgcccg gagggccggc aaagcaaagg atcgggtggc gatggctctc
     1741 cggattgaac ttgcgcaccg ctagatactc caggatgcgc tccttcacct tctcgatgcc
     1801 gtagtgatcc gcatcgagca cctcgcgcac ctcgcgcatg tcgaggttgt cctcggtgct
     1861 ggtgttccag gggagcgcga cgagccagtc gatgtaggta cgggcgacgg tgtactccgc
     1921 ggcttgagga ggcatctttg agaggcggtc caactcgcgg tccgcctcct tgcgtgcttc
     1981 ctcgggcatc tgagcggcct cgatcttcgc gcgcagctcc tcgatctccg cggtgcgctc
     2041 gtccgtttcg cccagctcct tctggatctg cttgagctgc tcgcggagat agtagtcacg
     2101 ctgcatcttc ccgagctcgg tatgcacctg cgactggatc ttcgtctgaa gctccaggat
     2161 ctccagatcg cggtggagca gcgaggcaag ccggcgcagg cgctcgcgca cgtcaatgat
     2221 ctccagcagc ccctgcttct cctggatcgg caggcgaata tgcgcgcaga tgagatccgc
     2281 gaggacatgg gcttcctgca cattctgtgg aatcgcctgc agctcgtcgg gcaggtcggg
     2341 cgagagcgaa accaccttct ggaagagggt gcccacgctg cgacgcagcg cctccagctc
     2401 catctgtgca tcctgaggaa gctctgcctc tggggtatcc tccggaagca cgcggacgcg
     2461 cacgcgcagg tacggctctg tctgcaagca ctccaggatc tcgatgcgcc ggagtccctg
     2521 cacgatgaga cggacgccct cgggcagctt cagcatggta tggatgacgg cagccgtgcc
     2581 gatccggtac acatcatcca gcgtcggctt ctcgatctcg ggcgacttca tcgccaccgt
     2641 accgatcacc cggctccctg ataccgcggc atcatcgatc agcttgacgg aactctcgcg
     2701 cgatacggcc agggggacca ccaccacggg aaagagcacg gtgtcgcgca gcggcagcag
     2761 gctcaactct tccggaatgc tgacgccgct ctcttcacca cccgtttggg ttggggtctg
     2821 tccctcattt acatccatcg cctatcctca ctgcctcaaa agcctcagtt catgggccgg
     2881 ctgtcacttc ccccttccgc cgtcacggcg gctcaccgcc tggcgggtca gtcgccagtg
     2941 acgggaatcg tgtgtaccac agagcgttct gctcgggcca ccttgggcaa aatgacaatc
     3001 aggaacccgt ctttgtaact accggtaatc ttctcgcgct cgacggccac accccccggc
     3061 agcggaatgc taatctcaaa cggcccaaag tagatctcaa aacggtacca acgcacgcgg
     3121 ccgacgcact cgccgcagtc ctcgacgcgt tggccgcgga tggtgagcac gcgtccatcc
     3181 ggcgagaggt cgacacgcac atcaggaagc gatccggtgt ccgggtcgac acgcagcccg
     3241 gcggcctcga ctttgacgac cagattgcgc tccgtctcgt agacatccgt gcgaggctgc
     3301 cagaaacggc caggcatcgg gtcgaagaag ccgcgccacc cttcggtcag gcgctccatc
     3361 tctttttcca tctgccggat gacgtcttca tagcgtgtaa agctcatcgc gccctaccca
     3421 tcctcatgag tgtgattgaa ctccgctctt cccgatagcg gcccggccgc ccactcggcc
     3481 ggcttccagc cgccggcgct atcgcgcacc ccgcacggca acgcgattcg ccatcaaacc
     3541 tcatcttagc acatacgcag tcgtacttca atacactgcg caactactgg gcaaaccccg
     3601 cggcattgcg ccgtctcact ccggacgggt cgctctcgcc tcaagagccg cagggaagag
     3661 caacgagggg cccttgcacg cacgccccgg cctctctgcc agactacccc aggccgcgtc
     3721 ctcctgtaag caggcggccg cagagctgac tcggcgaccg cctacgcctc ttctgccacg
     3781 cggccctacg cgatcttcat cgtcttgttt gccgcggccg cttccatcgc ggtgaggatc
     3841 acgttgaggg cgcgatatcc ctcttcacca tcgatcgcgg gtttcttgcc ggccaggatc
     3901 tcggccacga actggtccag cacgccgctg gagacctgct tcacgttcgt cgagatctcg
     3961 cccaccttgt gctgctccac atcaccgttg cgatagttca cgatgacgtt ccacaccgga
     4021 tcggtgccga tggagagcac gccgttctcg caatagagaa ccgtccagtt gtcctcggcg
     4081 ccatagttcg tccagctcac catgagggtg cccaggatac ccccttgcgt gcgcagggtc
     4141 aggaccgcgt tgtcatcaat atcgatgagc gagccatcgg ggtagcgctt gtggagcgtg
     4201 gtgatcacag cgctcacctc ggaaaactcg tcgccgagga gccagcgcat caggtctgcc
     4261 ttgtgaacgc ccaggtcacc cgtgacgccc aggctggcga tccccttctt gaagaaccag
     4321 gtgccggcgt ggttatcgac gctccagaac tccggtcctg ggtgcgcgaa tgcagtgcgg
     4381 aaagagatca ccttgccgag cttcccgctc tccaggatct gcttggcgca cacgtgcggg
     4441 ggcatcaggc gctggttgtg cccgatcatc aggttcttgc ccgccttctc cgcggcgtcg
     4501 atcatcgcgc gcgcttcttc gcgcgtggtc gccatctgct tctcg
//
LOCUS       DAOSCV010000286         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12040_length_4539_cov_3.811552, whole genome shotgun sequence.
ACCESSION   DAOSCV010000286 DAOSCV010000000
VERSION     DAOSCV010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12040_length_4539_cov_3.811552"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3176
                     /locus_tag="PLY56_08215"
     CDS             <1..3176
                     /locus_tag="PLY56_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21506.1"
                     /translation="DYSGRSVIVVGPELKLHQCGIPKEMALELFKPFVMKMLVEKGYT
                     SNIKTAKRMIDRMREEVWDALEEVIREHPVMLNRAPTLHRLGIQAFEPVLVDGKAIKV
                     HPLVCHAFNADFDGDQMAAHVPLSATAQAEARVLMLSTNNLFSPADGRPIVAPVQDIV
                     LGCYYLTVTRDAPLKEDVVYRPAEAMLAYEVGELDLHQLIRVRVPVTEREGTAEGNGE
                     VAERQETQEIETTVGRLIFNNILPEDMRYRAEFNRDLDKGAIARVVARCYEMHGSEAT
                     VKLLDDLKDLGFRFATRAGVSIASRDMNIQAGREEIIRRTEQQVASINQQYQDGFLTA
                     NEREENVLKYWTDAGEEIVNAILHKIDRFNPIYMMANSKARGSVKQISQLAGMRGLMA
                     DPFGRIIDSLPIKSNFHEGLNVLEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQ
                     DVIIRAVDCGTHDGIHVSRLLNEDDEEILSLRDRIRGRTAASTIFIQPDGTFVQIPPE
                     GINRADGETGEVLEQEQEPDLPVLVPRGEEISDAVAAKIEESGMKSVHIRSPLTCELR
                     YGICARCYGRDMATGRLAEVGLAAGIVAAQSIGEPGTQLTMRTFHTGGVAAEYLTGVA
                     EVKKKKQEALRELHADIRRGIIRFDVTGAEETGDTSAFDMSVGERERVRQVQELLKVL
                     EEQVKGLLRVVELFEARKPKGQAIITDIDGLVADIETHRLRQVIVHADLPIDGPDKVV
                     GEYAAKEIIDDETGEVLLRKGGEVTERVLRRLRELGKSTITVRRPHLVPYRGKLEVQV
                     GDHIRAGDRLTEGPLHPQDILKLQGIEGVQAYLVAEIQQVYKSQGVDINDKHVEVIVR
                     QMLKKRKVLDPGDTDLLPGQVVDKFELQAENVRVERLGGRPARAEYLLLGITEASLAT
                     ESFLSAASFQKTTKVLTDAAVKGKEDHLVGLKENVIIGRLIPAGTGMVPYRELRVTPT
                     DGDVSAFERLLPADEDLASETYTAARAVSILGEREERSSAAIHFPDVTAGTFEEVTEE
                     GSLGAELGSFEVSAPEATEEETDEDSE"
     gene            complement(3332..3880)
                     /locus_tag="PLY56_08220"
     CDS             complement(3332..3880)
                     /locus_tag="PLY56_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOJ21507.1"
                     /translation="MQVNQTTLTVVRGSVLDQETDAIVNAANTKMRGGGGVDGAIHRA
                     AGPELLEELKRVAPNGAPTGTAVLTGGHRLKQPYIIHTPGPVWHGGTRGEPELLASCY
                     RSCMEVAHKKGLESIAFCSISTGVYGYPIERAAPLALSTVRDFLQAHPDTSLRRVVFA
                     MFGAEEYRVFTEALGTLEKEGA"
     gene            4084..>4539
                     /gene="prs"
                     /locus_tag="PLY56_08225"
     CDS             4084..>4539
                     /gene="prs"
                     /locus_tag="PLY56_08225"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ21508.1"
                     /translation="MIAKRMRLFAGTAHPEFARKLGEHLGLTPDPSEAFHFTDGNLFV
                     KIGVNVRGYEVFLVQTLSPPVNDHLMELIFLIDACKRASSGPVTAVIPYYSYALADKK
                     DEPRVSIRGRIIADLLETAGVDRVLTMDLHSPQIQGFFRKPVDHLYAMPV"
BASE COUNT          834 a         1391 c         1544 g          770 t
ORIGIN      
        1 tggactactc ggggcgctcc gtcatcgtgg taggcccgga gctgaagctg catcagtgcg
       61 gcatcccgaa ggagatggcg ctggagctgt tcaagccgtt tgtgatgaag atgctggtgg
      121 agaagggcta cacaagcaac atcaagacgg ccaagcgcat gatcgaccgg atgcgtgaag
      181 aggtgtggga tgccctggaa gaggtcatcc gcgagcaccc cgtcatgctc aaccgcgcgc
      241 cgacgctgca ccgcctcggc atccaggcgt tcgagccggt gctggtggat ggtaaggcga
      301 tcaaggtgca tccgctcgtc tgccatgcct tcaacgccga cttcgacggc gaccagatgg
      361 cggcgcacgt gcccctgtcg gccacggcgc aggcagaggc gcgcgtcttg atgctctcga
      421 cgaacaacct cttctctcct gcggacggac gcccgattgt ggccccggtt caggatatcg
      481 tcctcgggtg ttactacctg acggtcactc gggacgcccc gctgaaggaa gacgtcgtct
      541 accgcccggc ggaggcgatg ctggcgtatg aggtgggcga gctggatctg caccagctga
      601 tccgggtgcg cgtgcccgtg acagagcgcg agggcacggc ggaggggaat ggcgaggtcg
      661 cagagcgcca ggaaacccag gagattgaga cgacggtcgg tcgcctgatc ttcaacaaca
      721 tcctgccgga agatatgcgc taccgggcgg agttcaaccg tgacctggat aagggcgcga
      781 ttgcccgggt tgtggcgcgc tgctacgaga tgcatggctc ggaagccacg gtgaagcttc
      841 tggatgacct gaaggacctg ggcttccggt ttgccacgcg cgccggcgtc tccatcgcct
      901 ccagggatat gaacatccag gccgggcgcg aggagatcat ccggcgcacg gagcagcagg
      961 tcgcgtctat caaccagcag taccaggatg gcttcctcac cgcgaacgag cgcgaggaga
     1021 acgttctgaa gtactggacg gacgccggcg aggagatcgt caacgccatt ctgcacaaga
     1081 tcgaccgctt caacccgatc tacatgatgg ccaactccaa agcgcgcggt tcggtcaagc
     1141 agatctcgca gctggccggt atgcgcggcc tcatggctga cccgttcggg cgcatcatcg
     1201 actcgctgcc gatcaagtcg aacttccacg aagggctgaa cgtgctggag tacttcatct
     1261 ccacgcatgg cgcgcgcaag ggcctggcag atacggcact ccgcacagcg gacgcgggat
     1321 acctgacccg gcgcctcgtg gacgttgccc aggacgtgat catccgcgcc gtggattgcg
     1381 gaacgcatga tggcatccac gtctcccggc tcctcaacga ggacgacgag gagatcctgt
     1441 cgctgcgcga ccggatccgc gggcgcacgg cggcatccac catcttcata cagccggatg
     1501 gtacgttcgt ccagatcccc ccggagggga tcaaccgtgc cgatggcgag acgggcgagg
     1561 tgctggagca ggagcaggaa ccggatctcc cggtgcttgt gcccaggggc gaggagatct
     1621 cggacgccgt ggcggccaag atcgaagaat cgggcatgaa gagcgtccat atccgctcgc
     1681 cgctgacgtg tgagctccgc tacggtatct gcgcgcgctg ctacggccgt gacatggcga
     1741 ccggacggtt ggccgaggtc ggcctggccg ccggaatcgt cgcggcccag tcgatcggcg
     1801 agccgggcac ccagctcacg atgcgcacct tccacacggg tggcgttgcc gccgagtacc
     1861 tcaccggcgt tgctgaggtc aagaagaaga agcaagaggc gctgcgcgag ctgcacgcgg
     1921 atatccgccg cggcatcatc cgcttcgacg tgactggtgc ggaggagacc ggggatacgt
     1981 ctgcgttcga tatgtccgtg ggcgagcgcg agcgtgttcg ccaggtgcag gagctcctga
     2041 aggttcttga ggagcaggtg aagggcctcc tgcgcgtcgt ggagctgttc gaggcacgca
     2101 agccgaaggg acaggcgatc atcaccgata tcgacggcct cgtcgccgat attgagacgc
     2161 accgcctgcg ccaggtgatc gtgcatgccg acctaccgat tgatggcccg gataaggtcg
     2221 ttggcgagta cgcggcgaag gagatcatcg acgacgagac gggcgaagtg ctcctgcgca
     2281 agggcgggga ggtgaccgag cgcgttctcc gtcgcctccg cgagttgggc aagtcgacga
     2341 tcaccgtgcg ccggccccac ctggtgccgt atcgcggaaa gctcgaagtg caagtgggcg
     2401 accatatccg cgccggcgac cgtctgacgg aaggcccgct gcacccgcag gatatcctga
     2461 agctgcaggg tatcgagggc gtgcaagcct acctggtcgc cgagatccag caggtgtata
     2521 agagccaggg cgttgatatc aacgacaagc acgtcgaggt catcgtccgt cagatgctga
     2581 agaagcggaa ggtgctggat ccgggcgata cggatctgct tcctggccag gtggtggaca
     2641 agttcgagct gcaggcggag aacgtgcgcg tggagcgcct cggtgggagg cccgctcgcg
     2701 ccgaatacct cctgctcggg atcacggagg cctcgcttgc gacggagtcg ttcctgtccg
     2761 ccgcgtcgtt ccagaagacg accaaggtgc tgacggatgc cgccgtcaag ggcaaggaag
     2821 accatctggt tggcctgaag gagaacgtga tcatcggccg gctgatcccg gcgggcacgg
     2881 ggatggtgcc ctaccgcgag ttgcgcgtca cgcctaccga tggcgatgtg agcgctttcg
     2941 agcgcctctt gcccgcggat gaggatctgg cttccgagac ctacacggcg gcgcgcgctg
     3001 tgagcatcct gggcgagcgc gaggagcggt cttcggccgc tatccacttc ccggatgtga
     3061 cggcgggcac gttcgaagag gtaaccgaag agggctctct cggggccgag ctgggatcct
     3121 tcgaggtctc tgctccagag gccacggagg aagagacgga cgaggatagc gagtagcact
     3181 gcgctatagc gcatgctgtg caagagcgcg ggggcggggc caacgttggc cccgcccccg
     3241 cgcttgttcg gagaggccgg gcgtgccggc cattcgtggc gtgcttaaga gcacggcgag
     3301 tcaccaccaa ccgtgccgat atcaggcgct gttatgctcc ctctttctcc agcgtcccga
     3361 gggcctccgt gaacacccga tactcctccg caccgaacat ggcaaagacc acgcgccgca
     3421 gcgacgtgtc gggatgcgcc tggaggaagt cgcgcacagt gctcagagcc aggggcgccg
     3481 cgcgctcgat ggggtagccg taaacaccgg tagagataga gcagaaggcg atgctctcca
     3541 ggcccttctt atgggctacc tccatgcagg agcggtagca ggacgccagt agctctggct
     3601 cgccccgggt gccaccgtgc cacaccggcc cgggcgtgtg gatgatgtac ggttgcttca
     3661 atcggtggcc accagtgagc accgcggtgc ccgttggcgc gccgttaggg gcaacgcgct
     3721 tcagctcctc caggagctct ggaccggcag cacggtgtat cgcgccatcc accccaccac
     3781 cgccgcgcat cttcgtgttt gcggcgttga caatcgcatc cgtctcctgg tcgaggacgc
     3841 ttccccgaac gacggtgagt gtcgtctggt tcacctgcat cgtgcgatcc ctcccaacag
     3901 gccggccctg gcaaggatgt catgggcgag gggccggctc ctcccaggcg gcattatacc
     3961 aggatggcac cgcgtgatca aacggcagca gagtgccggg gcgcggtttg ggcggcccct
     4021 gagacagggt atcctcaagg tggggcaggg catgaggacg ccactgtgtc gggaggacgc
     4081 cagatgatag ccaagcggat gcggctgttc gcgggcaccg cgcatccgga gttcgcgcgg
     4141 aagttgggcg agcacctggg gcttacgcct gacccctccg aggcgttcca cttcaccgat
     4201 ggcaacctct tcgtcaagat cggcgtcaat gtgcgggggt atgaggtctt cctggtgcag
     4261 acgctctcgc cacctgtgaa cgaccatctg atggagctca tcttcctgat cgatgcgtgt
     4321 aagcgcgcca gttcagggcc ggtcacggcg gtcattccct actactcgta cgcgcttgcg
     4381 gacaagaagg atgagccacg cgtctccatc cgcgggcgca tcatcgccga cctcctcgag
     4441 accgccgggg tggatcgcgt ccttacaatg gacctccatt cgccgcagat tcagggattc
     4501 ttccgcaagc cggtggatca cctctacgcg atgccggtg
//
LOCUS       DAOSCV010000287         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12124_length_4513_cov_3.263795, whole genome shotgun sequence.
ACCESSION   DAOSCV010000287 DAOSCV010000000
VERSION     DAOSCV010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12124_length_4513_cov_3.263795"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1591
                     /locus_tag="PLY56_08230"
     CDS             116..1591
                     /locus_tag="PLY56_08230"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF010322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOJ21509.1"
                     /translation="MSQTPARPNILLIMTDQHRGDCLGCAGHPVVETPYLDQLAAEGT
                     RFRCAYTAVPSCIPARATLMTGMHQWHTGILGMGRSGGNMTTAYRHTLPGELGRAGYH
                     TQGIGKMHFHPQRARYGFDNLILDESGRELSPGFKSDYRQWFEEHKDGAYDYRDHSVD
                     WNSWMSRPTHLPEHLHPTHWTAQQAIEWLGRRDPTQPFFLKVSFARPHSPYDPPQVYY
                     DLYRDRPLPEPHLGGWAHIHAGRPEQHDVNAWRSRRSPEEIRRARACYYGSITFIDHQ
                     IGRILYELQRYHREVWQNTLVLFLSDHGDMLGDHHLWRKTYAYEGSAHIPFIVRPPAS
                     WGWPRGQVLDHVVEIRDVMPTLLEAAGVPVPETVDGSSLLPLVQGQAETPWREYIQGE
                     HDWCYSHEQANFYVTDGRWKYIWFPYLGTEQLFNLESDPGECHNLAEDPACAQALAAW
                     RSRLVRELEERDCGLVRNGRLVRLELDQVIKSPHLYRYGCA"
     gene            complement(1725..3227)
                     /locus_tag="PLY56_08235"
     CDS             complement(1725..3227)
                     /locus_tag="PLY56_08235"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HOJ21510.1"
                     /translation="MEFDLLISGGQIVDGTGAPGFPGDVGIRGDRIEAIGDLSAAEAK
                     ERIDARGLVVAPGFIDPHNHANNEMAGGILELPSVENQIRQGVTTLVAGNCSDMARAW
                     PLDEHFEAVSRAPIRQNYAMLLGMGTLRAQVVKEWGRPANPDEIRQMQRMVEQGMDAG
                     ALGISTGYFPAFVTTDEIIEVTKPVAARGGLYASHIRNEGDGLLGAAEEIIEIGARAG
                     LPVQISHIKTYGQRNWWKVDALLSLMERASSRGVDVMADRYPYTACFTGLTSLLPMWA
                     QAETARRGGMSSLRDPAWYPDARRAIADQLDLIGGPQNVLFAPLKPQPEIEGKRLNEL
                     AEERGADVIDVAIDLLQQGGISCIYFLMCEENIRTFYRHPLVMAGSDSHLRVYGEGVS
                     HPRNYGTFPRIVGHYGRDEGLFGVEEAVRKCTSMAAERFRIKDRGVLAKGKYADLVLF
                     DWNRIIDRATFTDQHRYPEGIPWVIVNGQIAVREGEVTEGCYGRVIRRDE"
     gene            3388..>4513
                     /locus_tag="PLY56_08240"
     CDS             3388..>4513
                     /locus_tag="PLY56_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ21511.1"
                     /translation="MAVLKSFVRENPSAVFVRAYPYRSFEAIEADDGFHRGGYEAVAA
                     LFTEGEGTHRELLIKPNLTVGVSPELAANPAYHGGITTHPRFIAGLCDGLLERGERCL
                     TVVEGGIGLGVSVYAQTGYFAMMSEVVGEGPGAATREMRGVRLLWASKHRFEDFREDE
                     ITWVPVPNGVVHREIPVVTPFRKPGTALINAPTLKTHNLAVTTLCCKALQGMVAQGYK
                     HFCASLSAYDDPRAYPPEVLAHFQADFRERVLHEHARHCAMGLPYWSSNPDPVYGVGR
                     FECWAQRTADMLSAFQPFEEHFLLNVVEGIIGRNGTAFSQGEDVPVGLVVAGINPVHV
                     DAIASLLMGHDPQVIPYLVVARERGLGESDPTRIPVFRLPE"
BASE COUNT          741 a         1568 c         1440 g          764 t
ORIGIN      
        1 ggaggagaag ccctgaaagg gctttgcaaa gaagcagcgc ggagcttcga gctccgcgcg
       61 tgcgggcagc tctccttatt tcggaatctc agactgcccc cgaggggagt gaccgatgag
      121 ccagacaccc gccaggccga atatccttct gattatgacc gaccagcacc ggggcgactg
      181 cctcgggtgc gccgggcatc ccgtcgtcga gacgccctat ctcgaccagc tcgccgctga
      241 gggcactcgt tttcggtgtg cctacaccgc cgtgccctcc tgcattcccg cgcgcgccac
      301 gctgatgact ggcatgcacc agtggcatac cggcatcctg gggatggggc gcagcggcgg
      361 caacatgact accgcttacc ggcacaccct tccgggagag ctagggcgcg ccggctacca
      421 tacccagggc atcggcaaga tgcactttca cccccagcgt gcccgctatg gtttcgacaa
      481 cctgatcctg gacgaaagtg ggcgcgagct ctcgcccggc ttcaagagcg actaccgcca
      541 gtggttcgag gagcacaagg atggcgccta cgactaccgc gatcactctg tggactggaa
      601 ctcctggatg agccggccca cgcacctccc cgagcacctc caccccacgc actggaccgc
      661 gcagcaggcg attgagtggc tcggccggcg cgacccaacc cagccgttct tcctgaaggt
      721 gagcttcgct cggccgcatt cgccctatga cccgcctcaa gtctactacg atctctatcg
      781 cgaccggcca cttcccgagc cgcatctcgg cggttgggcg cacatccacg cgggccgccc
      841 ggaacagcac gatgtcaatg cctggcgcag ccggcgctct ccagaggaga tccgccgcgc
      901 gcgcgcctgc tactatggct caatcacctt catcgaccac cagattggcc gcatcctcta
      961 cgagctgcag cgctaccacc gcgaggtatg gcagaacacc ctcgtccttt tcctctccga
     1021 ccatggcgac atgctgggcg accatcacct ctggcgcaag acctacgcct acgagggctc
     1081 ggcccatatc ccgttcatcg tccgcccgcc agcgagctgg ggatggcccc gcggccaggt
     1141 cctcgatcac gtcgttgaga tccgcgacgt gatgcccacc ctcctcgagg cggcgggcgt
     1201 gccggtgccc gagaccgtgg atggcagcag cctcctcccc ctggtccagg ggcaagcaga
     1261 aacgccctgg cgggagtata tccagggcga gcatgactgg tgctatagcc acgagcaggc
     1321 gaacttctac gtcacggacg gacgctggaa gtacatctgg ttcccatacc tcggaacgga
     1381 gcagctcttc aacctggaga gtgatccagg cgagtgccac aacctggcag aggatcctgc
     1441 ctgcgcgcag gcactcgcgg cctggcgcag ccggctggtc cgcgagttgg aggaacgcga
     1501 ttgcggcctg gtgagaaatg gccgtctggt gcgcctcgag ctcgaccagg tgatcaagag
     1561 cccgcacctg taccggtatg ggtgcgcgtg atcgcccccc aatgtatcgg agggccgggc
     1621 gaaaggaggc accgcgctgc aaccctgcgc gcggatcggc ccggcccctc tcgcgcctgg
     1681 ctttccatcc ccgcggtcag aaggcaagga gggagagcgg aggcttactc gtcgcggcgg
     1741 atcacccgac cgtagcagcc ctcggtgacc tcgccttccc ggacggcgat ctggccgttc
     1801 acgatcaccc aggggatccc ctccggatac cggtgctggt cggtgaacgt ggcccggtcg
     1861 atgatccggt tccagtcgaa gagcacgaga tcggcatact tcccttttgc caagacgccc
     1921 cgatctttta ttcggaagcg ctcggcggcc atcgaagtgc acttgcgcac cgcttcctcc
     1981 accccgaaga ggccctcgtc gcgcccgtaa tgaccgacga tccgcgggaa ggtgccatag
     2041 ttgcgtggat gcgaaacccc ctcgccgtag acgcgcaggt ggctatcgga tcccgccatc
     2101 accagggggt ggcggtagaa ggtgcggatg ttctcctcgc acatcaggaa gtagatgcag
     2161 gagatcccgc cctgctggag gaggtcaatc gccacgtcga tcacgtcggc gccccgctcc
     2221 tcggcgagct cgttcaggcg cttcccctca atctcgggct ggggcttcag cggcgcgaag
     2281 aggacgttct ggggcccgcc gatcaggtcg agctggtccg cgatggcgcg ccgggcgtcg
     2341 gggtaccagg ccgggtcgcg tagcgagctc atcccgccgc gccgggccgt ctcggcctgt
     2401 gcccacatcg gcaggagcga ggtgagtccg gtgaagcacg ccgtgtaggg atagcggtcg
     2461 gccatcacgt cgacgccacg gctagacgcg cgttccatca ggctgaggag ggcgtccacc
     2521 ttccaccagt tgcgctggcc gtaggtcttg atgtgggaga tctggacggg aaggcccgcg
     2581 cgggcgccga tttcaatgat ctcttcggcg gcgccgagga ggccatcgcc ctcattccgg
     2641 atgtggctgg cgtagaggcc gccgcgcgct gccaccggct tcgtcacctc gatgatctca
     2701 tcggtggtca cgaacgccgg gaagtagccc gtggagatgc cgagcgcgcc ggcgtccatg
     2761 ccctgctcca ccatgcgctg catctggcgg atctcatccg ggttggccgg ccggccccac
     2821 tccttgacga cctgggcgcg cagcgtgccc atgccgagca gcatcgcata gttttgccgg
     2881 atgggcgccc ggctcaccgc ttcgaagtgc tcatccagcg gccacgccct ggccatgtca
     2941 ctgcagttgc cggcgacgag cgtggtcacg ccctggcgaa tctggttttc caccgaggga
     3001 agctccagaa tccccccggc catctcgttg ttggcgtggt tgtgcgggtc gatgaacccc
     3061 ggcgccacca ccaggccgcg tgcatcgatc cgctctttgg cctctgccgc gctgagatcg
     3121 cctatcgctt cgatgcggtc gccacgaatg cccacgtcgc caggaaagcc gggcgcaccc
     3181 gtgccgtcaa caatctgtcc ccccgagatc agaaggtcaa actccatcgt ggctcctctc
     3241 gcgcccgact ggcgcaggcg tcttgccttg ccgggcgccc tccctgatct tcgcacgcgt
     3301 ctcgcgtttc ccttccacgg tggcgatgcg gcatggggaa tgcacccagc ctggcggtgc
     3361 cccaggggcg ccagatggga gcaagcgatg gcggtgctga agtcgtttgt gcgagagaac
     3421 ccgagcgcgg tcttcgtgcg cgcctatccc taccggagct tcgaggccat cgaggctgac
     3481 gacggcttcc accgaggcgg ttacgaggcg gtggcggcgc tcttcacgga gggcgagggc
     3541 acgcaccgcg agctactgat caagccgaac ctgaccgtgg gcgtctcgcc cgaactcgcg
     3601 gcgaatcccg cctaccacgg cggcatcact acccacccgc gcttcatcgc cggcctgtgc
     3661 gacggcctgc tggagcgcgg cgaacgctgc ctcaccgtcg tggaaggggg catcggtctt
     3721 ggggtgagcg tctatgccca gaccggttac ttcgccatga tgagcgaggt ggtcggcgag
     3781 ggccccggcg cagccacgcg cgagatgcgc ggcgtgcgcc tcctctgggc cagcaagcac
     3841 cgctttgagg acttccgcga ggatgagatc acttgggttc ccgtgccgaa cggcgtcgtc
     3901 caccgcgaga tcccggtggt cacccccttc cgcaagccgg gcaccgccct gatcaacgcg
     3961 cccacgctga agacgcacaa cctggcggtg accaccctct gctgcaaggc gctccagggc
     4021 atggtggctc agggttacaa gcacttctgc gccagcctga gcgcttatga tgacccgcgc
     4081 gcctacccgc cagaggtgct cgcgcatttc caggcggact tccgcgagcg cgttcttcat
     4141 gaacacgcac gccactgcgc catggggctc ccctattgga gcagcaatcc cgatccggtc
     4201 tacggcgttg gccgcttcga gtgctgggca cagcgcacgg ccgatatgct ctctgccttc
     4261 cagcccttcg aggagcactt cctcctcaac gtggtcgagg ggatcatcgg gcgcaacggc
     4321 acggcgttca gccagggcga ggatgtgccc gtcggcctgg tggtggccgg catcaacccg
     4381 gtgcatgtcg atgcgatcgc gagcctcctg atggggcacg acccccaggt gatcccctac
     4441 ctcgtggtcg cccgggaacg cgggctcggc gagagcgacc caacgcgcat cccggtcttc
     4501 cgcctcccgg aga
//
LOCUS       DAOSCV010000288         4512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12126_length_4512_cov_3.581108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000288 DAOSCV010000000
VERSION     DAOSCV010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12126_length_4512_cov_3.581108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..465)
                     /locus_tag="PLY56_08245"
     CDS             complement(142..465)
                     /locus_tag="PLY56_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21512.1"
                     /translation="MWRAIVPAILGACLALGFPAVGSAEAAEETGDQAQPTVVVVPAV
                     PSVVYVGNIPLLLTSRQAVVVRGGARAVIVPVHGRYLVNGTPVAILRPIQVIVVDLSA
                     AAPET"
     gene            complement(629..1807)
                     /locus_tag="PLY56_08250"
     CDS             complement(629..1807)
                     /locus_tag="PLY56_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ21513.1"
                     /translation="MNRTNTTCRALALLARAARSLALAVPICLCAGHMGFAASSEKGT
                     ASTPPQIPLCMIGDSITWSGHGDTWRQFLLEQIPTLAFVGTHTAKLGYSHAGEGGNST
                     AKVLARIDAIPDCPYYHLHIGTNDNNTKDPALVERTAAGTAERIIAIVNALLKKPSVK
                     KVFLASVFPCHTDNPLRDQTNARTNVFLRERFATAFPPDKVVWVEYEKPIRETKNWEA
                     MILLHPTPEGYRLVARILADALREALNLPPSLPAPRPQPGAGVRVVNLWRGGSDGQTT
                     EPIIAGWYTLSFDVVSTDPAGGTVTIQNATEVEKRLRHATAIKPADAGKRIAINFFTG
                     YEGYGYTRTPLSLDVQGCRIDQILLEKQRPSGKASRYGVGSYLDTESPFSLGELLELP
                     "
     assembly_gap    2126..2135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2136..>4512
                     /locus_tag="PLY56_08255"
     CDS             <2136..>4512
                     /locus_tag="PLY56_08255"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOJ21514.1"
                     /translation="VTLLKAAPDPPFFERLATRLLECLAECADPDMALGNLHRWLGQL
                     GHSLAVQRMLVEDQFLLRHLLLLFSSSQYFADILCRDPAYYELLADPARLAQRRTAKE
                     IAEAVARSVAPFRTYEGRLDALRRAKRREILRIGARDLFGLASFPEVVDELSAFADAA
                     VAQAYRMAREEIERRFGSLDGLEFAVIAMGKLGGRELNYSSDIDLIFVYRGSEEHVAS
                     AARIGEHLIEALARPTAEGYLFRVDMRLRPEGQAGPLVRSLDAYRDYYEHHIEHWERQ
                     ALLKARFVAGDETLGRDFHALIEPYVYRLDVPPSLIEELRLNKLRVEKRCEMAGERFT
                     NVKDGWGGIRDIEFTVQLLQLLFGARVPEVRTGNTLEALDALARAGLLPAEECQRIRE
                     AYEFLRVVEHRLQIMDERAVRCLPLASEAVARLARRMPRMWHHDPGAEFTAEYRRHAG
                     TVRGFHQRFFFDPFEREAATPSRLTALGELLLTLDSEDARARIRQYLVARGFLAPERA
                     LSDLDRLAGIGGPRAKAARLEDLALLARVGPQLLETAAMAPDLDAALSFVVEVALKGD
                     REGLLTTLGESGFAREVMCRLGGTAPAATQPIRREPALLDMLFEPEAIASPADEHSLR
                     ERLAIRLARHEDHEARLNSLRRFKRREMLRIAAADVLAAVDIEGVVHQLTLLAEVCVG
                     AAVDLSVDALRARGDLEGEPEFAVFGLGGVGGFEMLYPSDLDLLFVCDLPEGEARERC
                     YLSYSRLAERTQDALTRLLPDGDLYPVDLRLRPEGQGGELALGRNVLVAFVS"
BASE COUNT          697 a         1430 c         1572 g          803 t
ORIGIN      
        1 cagaagaacc cgcgccatgc ttggcaaaga gatttttcat tatactgatg aatcgtgcgg
       61 gctagctcgc ctggccgagg cctcctcaag gggatccgcc aggcaagctg cccgataacg
      121 agctttctcg caccacgcgg gctaggtttc gggtgctgcc gccgacaggt cgacgacgat
      181 cacctgaatt gggcgcagaa tggcgacggg cgttccgttg acgaggtatc gcccatgcac
      241 tgggacgatc accgcccggg cgccacctcg gaccacgacg gcctgacggc ttgtcaggag
      301 cagcgggatg ttgccgacgt acacgacgga cggcaccgcg ggcacgacga caacagtcgg
      361 ctgggcctgg tcgcctgtct cctccgcggc ctcagcggat cccaccgccg gaaaacccag
      421 ggcgaggcat gcgcccagga tcgccggaac gattgctctc cacacggtcg gctgcctcct
      481 ctcggcggtg accacgggtc ccgccgcgac gcgagcaccg atcgattacc cgtaccggga
      541 gggagcaaac cggcgtggtg cgcctctcgc gagccggcaa aaagggcagg gggcagacgc
      601 gacccacgcc gctgcgtaac cttcgtgcct acggaagttc cagcaactcg cccagggaga
      661 agggtgactc cgtgtcgagg taggaaccga cgccgtagcg cgacgctttg cccgaaggcc
      721 gttgcttctc caggagaatc tgatcgatcc ggcagccctg cacgtcgagg gagagcggtg
      781 tccgggtgta gccatagccc tcgtacccgg tgaagaagtt gatggcgatc cgcttcccgg
      841 catcggcggg cttgatggcg gttgcgtgtc gcagtctctt ctccacctcc gtggcgttct
      901 ggatcgtcac cgtgcctccc gcgggatcgg tggagaccac atcgaaggag agcgtgtacc
      961 acccggcaat gatcggctct gtcgtctggc catcgctacc gccacgccac aggttcacca
     1021 cgcgcacgcc tgcaccaggc tgggggcgcg gtgctgggag cgagggcggc aggttcaacg
     1081 cctcgcgaag cgcatccgcc agaatccgcg ccaccaggcg atagccctcc ggcgtcggat
     1141 ggaggaggat catggcctcc cagtttttcg tctcgcggat aggcttctca tattctaccc
     1201 agaccacctt gtccggcgga aaggcggtgg cgaagcgctc gcggaggaag acgttcgttc
     1261 gcgcgttcgt ttggtcgcgc agcgggttgt cggtatgaca ggggaagacg cttgcgagga
     1321 agaccttctt cacgcttggc ttcttcagga gcgcgttgac gatagcgata atccgctccg
     1381 ctgtgcctgc cgcggtgcgc tccaccaacg ccggatcctt tgtgttgttg tcgtttgtgc
     1441 cgatatgcag gtggtagtat gggcaatcag ggatggcgtc gattcgcgcg aggaccttgg
     1501 ccgtgctgtt tcctccctcg ccggcgtggc tatagcccag cttcgcggtg tgcgtgccga
     1561 caaaagcgag cgttgggatc tgctccagga gaaactggcg ccaggtgtcg ccatgacctg
     1621 accaggtgat gctgtcgcca atcatgcaga gggggatctg cggcggtgtc gaggccgtgc
     1681 ctttctcaga agacgcggca aagcccatgt gtcccgcgca caggcaaatg ggcaccgcga
     1741 gggcgagaga gcgcgcggct cgcgccaaga gtgcaagcgc gcggcaagtg gtgttggttc
     1801 ggttcatact gcctcctctg gactattgct tcgtcgcaga aggtcatgtc gcctgcagga
     1861 tcttatggta cttcctacca tccggcccct gcaggttccg agaccgcgga tggaagagcc
     1921 gtaaggcgtt ttcgaaggac cgcgaagcgc accggcattc cggccgggtc tcgcgatctg
     1981 ggggagggca catggtgctt gccagcgccg cgtgattgtg tcataatgac gggggggagt
     2041 tgatggacgc ggcgctcgag agttttggat tggacctgtc ggcgggaggc ttgcggaata
     2101 cggcgagcgc ccggcaggtg tttgtnnnnn nnnnngttac cctcctgaaa gcggccccgg
     2161 atcccccctt cttcgagcgc ctggcgacgc gcctcctgga gtgcctggcg gagtgcgccg
     2221 acccagatat ggcgctgggg aacctccacc ggtggcttgg gcagctcggt cacagcctcg
     2281 ccgtgcagcg catgctggtc gaggaccagt tcctcctgcg ccacctgctc ctgctctttt
     2341 cgtccagcca gtacttcgcc gatatcttgt gccgcgaccc cgcgtactac gagctgttgg
     2401 ccgatccagc gcggcttgcc caaagacgca ccgcgaaaga gattgccgag gcggtggccc
     2461 gcagtgtggc gcctttccgc acctacgagg gccggctgga tgccctgcgc cgcgccaagc
     2521 ggcgcgagat cctgcgcatc ggcgcgcgcg acctcttcgg cctggcgagc ttcccggagg
     2581 tcgtggacga gctctcggcg tttgccgatg ccgccgtggc acaggcctac cggatggccc
     2641 gcgaggagat cgagcggcgc ttcggttccc tggacggcct ggagttcgcg gtcatcgcca
     2701 tgggaaagct gggtgggcgc gagctcaact actcctccga tatcgacctc atcttcgtct
     2761 accgggggag tgaggagcac gttgcctcgg cggcgcggat tggcgagcat ctcatcgagg
     2821 cgctcgcacg gcccacggcg gagggctatc tgttccgggt cgacatgcgc ctgcgccccg
     2881 aggggcaggc cgggccgctg gtccgctcgc tcgatgcgta tcgcgactac tacgagcacc
     2941 acatagagca ctgggagcgg caggcgctgc tgaaagcgcg cttcgtggcg ggcgatgaga
     3001 cgctgggccg cgacttccac gcgctcatcg agccctatgt ttaccgcctg gacgtccccc
     3061 cctcgctgat tgaggagctg cgcctgaaca agctgcgcgt ggagaagcgg tgcgaaatgg
     3121 cgggcgagcg cttcaccaac gtgaaggatg gctggggcgg gattcgcgac atcgagttca
     3181 ccgtgcagct cctgcagctt ctcttcggag cgcgcgtgcc ggaggtgcgc accggaaaca
     3241 cgctggaggc gctggatgcg cttgcgcgtg cgggcctgct ccctgccgag gagtgtcagc
     3301 ggattcgcga ggcctacgag ttcctgcgcg tcgtggagca ccggttgcag atcatggatg
     3361 agcgcgcggt ccgctgcctc cccctggcct ccgaggcggt ggcgcgcctc gcccggcgaa
     3421 tgccgcgaat gtggcatcac gatccaggcg ctgagttcac cgccgagtac cggcgacatg
     3481 ctggcaccgt gcgcggcttc caccagcgtt tctttttcga cccgttcgag agagaagcgg
     3541 cgactcccag ccggttgacg gcgctgggcg agctgctcct cacgctcgat tcggaggacg
     3601 cgcgggcgcg catccggcag tacctggtgg cgcgcggctt cctggcgccc gagcgcgcgc
     3661 tgtccgatct ggaccggctg gcggggatcg gcgggccacg agccaaagcg gcgcgcctgg
     3721 aagacctggc gctgctggcg cgcgtcggac cgcagctttt ggagacggcg gcgatggctc
     3781 cggatctgga tgcggcgctc tccttcgtgg tggaggtggc gctgaaggga gatcgcgagg
     3841 gcctgttgac cacactcggc gaaagcggat tcgcgcgcga ggtgatgtgc cgtttggggg
     3901 gcaccgcgcc cgcagcgacg caaccgatcc ggcgcgaacc ggcgctcctc gacatgctgt
     3961 tcgagccgga ggcgattgcc tcgcccgcgg atgagcactc gctccgagag cggctggcaa
     4021 tccgactggc gcgccatgag gaccacgagg cgcgcctaaa ctcgctccgg cggttcaagc
     4081 gccgggagat gctccggatc gcggccgcgg atgtgctggc cgccgtggac atcgagggcg
     4141 tggtgcatca actgacgctc ctcgcagagg tgtgtgtcgg cgcggcggtg gacctgagcg
     4201 tggacgcgct tcgggcgcgg ggcgacctgg agggcgagcc ggagttcgcg gttttcggcc
     4261 tcggcggagt tggcggcttc gagatgctct acccatccga tctcgacctc ctcttcgtgt
     4321 gcgatctccc cgagggcgag gctcgcgagc ggtgctacct gagctactcg cgcctggccg
     4381 agcggaccca ggatgccctg acgcgcctcc ttccggatgg cgacctctat ccggtggacc
     4441 tgcgtctccg tcccgagggc caggggggcg agctggccct gggtcggaac gtgctggtgg
     4501 ccttcgtctc ct
//
LOCUS       DAOSCV010000289         4511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12129_length_4511_cov_2.917864, whole genome shotgun sequence.
ACCESSION   DAOSCV010000289 DAOSCV010000000
VERSION     DAOSCV010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12129_length_4511_cov_2.917864"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            341..1387
                     /locus_tag="PLY56_08260"
     CDS             341..1387
                     /locus_tag="PLY56_08260"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOJ21515.1"
                     /translation="MKILCYGAGAVGCLISGRLSAASHDVLLLSRAEAAAEIRAHGLA
                     IEEPDGRIIYARPRVITSLAQMRVDEAPPDLIIAAVKAYDLPEVIRDIAQACRAETPV
                     LTLQNGIGSEEAAIDVLGSERVVAGSFTLNVSLPEPGRVAQHTRSGGIALAEAHPSGK
                     RLRSLVDLFTTSDIRAQAYPDWRAMKWSKLLLNLLANASCAILDVTPAEVLLHPALFR
                     MERAAFLEARRVMRRLHLRPVDLPGYPVRRLAFAMGLPAPLARRLVAGRAAGGRGSKP
                     PSLALDLARGKGQSEVRFLNGAVARVASEQGMRAPVNASLASTLEALAQGAADHATYR
                     HSPKALLAECGLSR"
     gene            complement(1590..3944)
                     /locus_tag="PLY56_08265"
     CDS             complement(1590..3944)
                     /locus_tag="PLY56_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOJ21516.1"
                     /translation="MPAWSSFIAGKKAKLNIDFRGAEIDNVLKFFSMAAGVTISKDPA
                     LKGPVTIVNAKQLSLDEAFRVLDAVLNSKGYRLLKEGPLLRIAAAAPQAPPVQWPSPG
                     GFGRAEGPEERPNEIRVFELQHADAQQVARVINDLFPNRGGLAAMARARRTRRDGQQA
                     GMPMVPTGPEVRASADDYTNSVVVNANPEYISKVEVLIRNLDKEVTSTMQTRVFPLEY
                     ASATEMADVISSVLLSTAPGQRTAASQSLPFEQRIRGGGGRTAFYAVSAQVVPNERTN
                     SLIVTATPSNLAIAEMVIQELDKPVEFESTTYVLPLANAQAMDVAYLLDQALRTGGTS
                     SSYYSQQRYRGTTQRRTTTYRPQSYQRRSAASDAPARSRAQASLDEVRLPDALDEEAL
                     SEEGLDGADPASSRQFPGWRGGAGSSGATVRVGRAGSGQIVNLLDLAGQANVVADPNT
                     NSLIITTTPENYDAIKEIVKQLDTVPEQVLIEALVVEAALDSTSKLGMEWSWMQENVA
                     GASVGANVKMLPQDLAQERTNFKFSVIGPSLQTVLTALSTDKRFNVLSTPRIFTANNR
                     QAQINISQRVPYIMSTRETDAGTVTYNYGYLDVGLVLDVTPHITQNGMVNIEVTQEAN
                     ELQGFTSYQAPLISQRSATTTITVRDGETVVLGGMIREQTDRTVNKTPILGDLPILGG
                     LFRSRGVTKGKTELMIFLTPRVVRDVEQAAALSRDQQRQVKIVLPSSAVPPTDAVAPN
                     GVQPAAAVPPTDTVAPNGAQPAPVPPSNTASPNAVQSAPEPAKP"
BASE COUNT          699 a         1485 c         1513 g          814 t
ORIGIN      
        1 cggtatcctc cacccgaacc gcttcgtccc ccccaaaagg cgtgatcagc acggggacca
       61 ttccatcgat cggcagcatc cgaccctcca ggcagaggtg acctgcaagc gccacgtgcg
      121 acgcctgcaa cccctgtcct ctggagagca gcgtgcgcgg ggaactcacc cgctctcccc
      181 ttcctgcttc tgtccgggcc aggcgattcc tgccggtcgc gaagaggctt cgcagggcag
      241 aatcagttca gggcaggcgc gccggcctgc cggttgtatc ctgcggaatg ggccgacacg
      301 cgcgccagga tcccagcgcc tgcaggcaga atcttgaggc gtgaagatcc tatgctatgg
      361 tgcaggtgcg gttgggtgtc ttatcagcgg ccggctctcg gccgccagcc atgatgtgct
      421 gttgctctcg cgcgcggagg cggccgcgga aatccgagct cacggcctgg cgattgagga
      481 gccggacggg cgaattatct atgcccgacc gagagtcatc acctctctcg cacagatgcg
      541 ggtcgatgag gcgcctcccg atctgatcat tgccgcagtc aaagcatacg acctgccgga
      601 ggtgatccgc gacatcgcgc aggcttgcag ggccgagacc cccgtgctca cgctccagaa
      661 tgggatcggc agcgaggaag cggcgattga cgtgctcgga tccgagcgcg tggtggccgg
      721 ctccttcacc ctcaatgtga gtctcccaga acctggcagg gtggcgcaac acacgcggtc
      781 cggtgggatc gccctcgcgg aggcgcaccc ctcgggaaaa cgcctgagat ccctcgtaga
      841 tctattcacc acgtccgaca tccgggccca ggcctatccg gactggcgag cgatgaagtg
      901 gtccaagctc ctcctcaacc tactcgccaa cgcctcatgc gccatcctgg acgtgacgcc
      961 ggcggaagtg cttctccatc cggccctctt ccgcatggag cgcgccgcct tcctggaggc
     1021 gcgtcgggtg atgcgccgcc ttcatcttcg cccggtggat cttcctggct atccagtgcg
     1081 ccgcctcgcc ttcgcgatgg gcttgccagc gcccttggcg cgcaggctgg tcgccggccg
     1141 cgcggcggga ggccggggaa gcaaaccccc ctccctcgcc ctggatctgg cccggggaaa
     1201 gggtcagtcg gaggtccgct tcctgaatgg cgcggtcgct cgcgtggcga gcgaacaggg
     1261 gatgcgggcg cccgtgaacg cttcgttggc ctccaccctg gaagccctcg cccagggcgc
     1321 cgcggatcac gccacatacc gccactcccc gaaggcactc ctggccgaat gcggtctgtc
     1381 gaggtagcac cggcgcgttc aggcgccacc tggcgagatg ctggacgagc agcccctctt
     1441 gtatcagtag ctctgggtga agggcctctg gtcgtcgggc acgccaggac caggcgcgcc
     1501 ccagggcacg gggaacgcat gagggacgcg agtgcagtgg cgatctggcc ccccttccgc
     1561 gccggaaggg gggccagtct atccgtgggc tacggcttcg ccggctcggg tgccgactga
     1621 accgcattcg gagacgctgt gttggagggt ggtacgggtg ccggttgtgc cccattcgga
     1681 gccaccgtgt cagtgggcgg cacggcggcc gccggctgaa ctccattcgg agccaccgca
     1741 tcggtgggcg gcacagcgct ggatggcagc acgattttca cctgccgctg ctggtctcgc
     1801 gacagggcgg ccgcctgttc cacgtcgcgc accacgcgcg gcgtcaggaa gatcatcagc
     1861 tccgtcttcc ccttggtgac gccgcgcgac cggaacagcc cgccgaggat cgggagatcg
     1921 cccaaaatcg gcgtcttgtt taccgtgcgg tctgtctgtt cgcggatcat gccgcccaga
     1981 accaccgtct cgccatcacg caccgtgatg gtggtggtag cggagcgttg gctgatcagc
     2041 ggcgcctggt agctggtgaa gccctgcagc tcgttggcct cctgggtcac ctcgatgttc
     2101 accatgccgt tttgcgtgat gtgcggagtc acgtcgagca cgaggccaac atccaggtag
     2161 ccatagttgt aggtgacagt tccggcatcc gtttcgcgag tgctcatgat gtagggcacc
     2221 cgctggctga tattgatctg cgcctgccgg ttgttggccg tgaagatgcg cggcgtcgaa
     2281 agcacattga agcgcttgtc cgtcgatagc gccgtcagca ccgtctgcaa cgagggcccg
     2341 atcacagaga acttgaagtt ggtccgttcc tgtgccaggt cttgcgggag catcttgacg
     2401 ttggctccca ccgaagcgcc cgccacgttc tcctgcatcc aggaccactc catgccgagc
     2461 ttgctggtgc tatcgagcgc ggcttccacg accagcgcct cgatcaacac ctgctctggt
     2521 acggtgtcga gttgcttgac gatctctttg atggcgtcgt agttttccgg ggtcgtggtg
     2581 atgatcaggc tgttggtgtt cggatcggcg acgacgttcg cctggcccgc caggtcaagc
     2641 aggttgacga tctggccgct tccggctcgc cccacgcgca cagtggcgcc tgaagagccc
     2701 gcgccaccgc gccaccctgg gaactggcgc gaggaagcag gatccgcgcc gtccaaaccc
     2761 tcctccgaca acgcttcctc atcgagcgca tccggcaatc gcacctcatc cagacttgcc
     2821 tgagcgcggc tccttgccgg cgcatcgctc gccgcggagc ggcgctggta gctctgcgga
     2881 cggtaggtgg tggtgcgccg ctgtgtggtg ccacggtagc gttgctgcga gtagtagctg
     2941 gaggaggtcc cgccggtgcg gagagcctgg tccaggagat acgcgacatc catggcctga
     3001 gcgttggcga gaggcagcac gtaggtggtc gactcgaact ccaccggctt atccagctcc
     3061 tggatcacca tctccgcgat ggcgagattc gatggggttg ctgtgacgat gagcgagttc
     3121 gtgcgctcat tcggcaccac ctgggcgctc acggcataga acgccgtccg gccaccaccg
     3181 ccgcggatcc gctgttcaaa cggcaggctc tgcgacgcgg cggtgcgctg cccgggcgcc
     3241 gttgacagga gaacgctgga gatcacatcg gccatctcgg tggcgctcgc gtactccagc
     3301 gggaagacgc gcgtctgcat ggtgctggtc acctctttgt cgaggttgcg gatcagcact
     3361 tccaccttcg agatgtactc cggattggcg ttcaccacga cggagttggt gtagtcatcc
     3421 gcgctcgcgc gcacctccgg tccggtcggc accatgggca tgcccgcctg ctggccatct
     3481 ctccgggtgc ggcgcgcgcg cgccatcgcc gcgaggccgc cccggttggg gaagaggtcg
     3541 ttgatgacgc gggccacctg ctgggcatcg gcgtgctgca gttcgaagac acggatctcg
     3601 ttggggcgct cttccgggcc ctcggcgcgg ccaaaaccgc caggtgaagg ccactgtacg
     3661 ggaggcgcct gcggcgccgc ggccgctatg cgcagaagcg ggccttcctt caacagccga
     3721 tagcccttgg agttgagcac agcgtccagg acgcggaacg cctcatccaa cgaaagctgc
     3781 ttcgcattca cgatcgtcac cggccccttg agcgccggat ccttgctgat cgtgactcca
     3841 gccgccatgc tgaagaactt caacacatta tcgatctccg cgccgcggaa atcgatgttc
     3901 agttttgcct ttttgccggc gataaaggaa ctccacgccg gcaccgccgg ggttccctgc
     3961 cactccgtgc cgcttggcgc ggaggttggg aacgggatcc tgggcggagc cgtggatgat
     4021 cctgacgctg gtgaagccgc cgatggggcc ggagtggctg gtgccggagc gggtggcagg
     4081 cccgaaggct gcgcaggcac cgcggtctcc gttccctgcg gagttgcagg cgcgggggcc
     4141 gcactcgcgg gctgcgcggg cgcggatgga gctggttgcg ccgaggaggg agcggtgggc
     4201 gcggcctccg cctgcttctc gcccgagggg gctggctggg caggagctgt ctctgccggc
     4261 ttgggagccg gggcaggcgg cgcctgctcc tcggttccag ccggggcaag aaccgccagc
     4321 gccaggcaga ggaccagaga tcccggccgc aggagccatc tttggcgcga gggtaagggc
     4381 atcacatcgg ttctcctgtg catcattggg tctgcctctc aaccggagta gagctgggga
     4441 ccgtggcggt cccctctcca cgacgccacc tcaaataacg ttcgcggcgg gcggcgcgtt
     4501 ccgcattcgc g
//
LOCUS       DAOSCV010000290         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12259_length_4473_cov_3.270258, whole genome shotgun sequence.
ACCESSION   DAOSCV010000290 DAOSCV010000000
VERSION     DAOSCV010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12259_length_4473_cov_3.270258"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..753
                     /locus_tag="PLY56_08270"
     CDS             259..753
                     /locus_tag="PLY56_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOJ21517.1"
                     /translation="MNWTDLVVLIIIGSITAAEAKRGLGPALFTVAGALLSARLALLW
                     SGPVSGSLRLTANPGQNQAWALFLLFALLMTVVLAIIHVMHPETMISADPFDGPLGAI
                     CGFFTGCAVAFVFYKGLGFWGARDLLQSSLFAPEILHQRTYHAIVRGLYGLGDAQNER
                     LNIK"
     gene            802..1197
                     /locus_tag="PLY56_08275"
     CDS             802..1197
                     /locus_tag="PLY56_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21518.1"
                     /translation="MQDELQKMEEIRNRLGVSYEEARYALQQAGGDVAAAMVIAEQDR
                     ETAGGGLAAASMAFMDELQEFISQGEMRKVRVKLGHRLLKEFDVSPRTAIAMLGIAVA
                     AVLLTKLRIEVERAPEAAGELPASGFSQE"
     gene            1438..1989
                     /gene="lepB"
                     /locus_tag="PLY56_08280"
     CDS             1438..1989
                     /gene="lepB"
                     /locus_tag="PLY56_08280"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ21519.1"
                     /translation="MRKHSPTWRDWLHTLVVAGGLFLFTETFLLQGFSVHGSCMEPNL
                     YTGERLLGNKLRYRLHPPQRGDVVVFRYPDNPRKIYVKRVVALPGEMIAIRDGTVYLN
                     GKPLHESYVINPAHGSYPPARVPSGQYFVLGDNRDHSSDSRAWGFVPTRHLEGKVWLR
                     YWPLRRAGILPAGRSCPSHGEQS"
     gene            1986..4097
                     /gene="glyS"
                     /locus_tag="PLY56_08285"
     CDS             1986..4097
                     /gene="glyS"
                     /locus_tag="PLY56_08285"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOJ21520.1"
                     /translation="MKELVLEIGVEDLPPAAISPALAEMKQLLGERLRQENIRYQEIR
                     TYGTPRRLVACVTGVAPRQPDTVIEVRGPSAQQAFDSFGNYTYAAFSFARTQDVFPEE
                     LKVKRITGGEYLFVERPKPGRPTVEVAAGFLPELITNLAFLRMARRDESAPRSPIRWL
                     LALYGEDVIPFQVGDVEAGRLTRGHRALHGCTPEISATIEVPKASAYLELIRAAGVIL
                     DPEERRQRILEQARALAQAVGGQPLEEALADELAFLVEQPAAFAGGFDPAFLVLPREA
                     LVLVMQKQQRLFPIVSPEGALLPRFIGIHDGSPEGVEEARAASEQVLHARLSDLRLSY
                     EQDLRTPLPARVDALHSIPYQERLGTLFEKTERLGSLVEELCDRWGVDATTRQNALRA
                     ALLAKADLATDLLKELPELRGRIGQAYALHAGEPPAVADALAGHYLPRVAGDPLPQTE
                     AGRILGVADRIDTLVGHFGMGLVPIGSQDPHALRQQASGLVAILAESERLSLGEMVEL
                     AYGLYGDRGPGWRSLDVTRASLLDFLRQCLESLLSEHGVRYDTADAVLDAGFDDVRGT
                     LIRAQLLEERRDAPEFAETLMAAARLGSLLRFVARSEMAVPPGEVRIDLLQEEAEQLF
                     YAAYLDVCPLVTEAAERGDYAAALDALGGLAAPALEFFDNVLVTSDDELLRANRVRLL
                     GEIHALFLKIADLTRLTVEIE"
     gene            4227..>4473
                     /locus_tag="PLY56_08290"
     CDS             4227..>4473
                     /locus_tag="PLY56_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21521.1"
                     /translation="MEFEIKPFDVSHKAQILGLSLRAWAPVFSGLRPAVPEYVYEAFY
                     PHGWRARQRSDIEAFLDTEGDRVWVAVKERAVLGWVGI"
BASE COUNT          707 a         1478 c         1475 g          813 t
ORIGIN      
        1 caggctgttc cacgtattcc atctctcatg cgcgcccccg actggggcag gtacgcttct
       61 attcgccatc gccctgccag cttccttgct caatcccgct tgtttctgga ggcgcaggag
      121 agtgctccac gctgccgaat cctggagata gacatacacg gataaacgtt ctagcatcgc
      181 ctctctggga gaccgggcgc gcgtcatgtt gactttcact caggcgtcgt ttataatggc
      241 gctggcagga ggggcacgat gaactggact gatctcgtcg tgctgatcat catcggcagc
      301 atcacggccg ccgaagccaa gcggggcctt gggccggcgc tcttcaccgt cgccggcgcg
      361 ctgctttccg cccggctcgc cctcctctgg tctggtccgg tctccggctc tttgcgtctg
      421 acggcaaacc ccgggcagaa ccaggcgtgg gcgctcttcc tgcttttcgc gctcctcatg
      481 acggtcgtgc tggcgatcat ccacgtgatg catccggaaa cgatgatctc cgccgacccg
      541 ttcgatggcc cgctcggtgc catctgcggg tttttcacgg ggtgcgccgt ggccttcgtc
      601 ttctataagg gcctggggtt ctggggcgcc cgagacctgc tccagagttc tcttttcgcg
      661 ccggagatat tgcaccagcg cacctatcat gccatcgtgc gcggtctcta cggcctcggg
      721 gacgcgcaga acgagcgtct gaatatcaag tgaccggttg cgtggcgccg ggaagagcgt
      781 cgcgggaagg aagggcaacg catgcaggat gagctgcaga agatggagga gatccgcaac
      841 cgcttgggag tgtcctacga ggaggcgcgc tacgcgctcc agcaggcggg cggcgatgtg
      901 gcggccgcca tggtgattgc cgagcaggat cgtgagacag cgggcggcgg cctggctgcc
      961 gcgagcatgg cgttcatgga tgagctgcag gagttcatat cccaaggtga gatgcggaag
     1021 gtccgcgtga agctgggcca tcgcctcctc aaggaattcg acgtctcgcc gcgcacggcc
     1081 atcgccatgc tcgggatcgc ggtggccgcg gtgctgctca ccaagctccg catcgaggtt
     1141 gagcgcgccc ccgaggcggc aggcgaactc ccggcatcgg gtttcagcca agagtgacgc
     1201 ttcaggagct catcgccgcg ctcgacggct cctgggctgc gcaggggtgt gtcgtggcac
     1261 cggcctacga tctcaaggtg ggcacgggca ggatgcatcc catcgcctcc ttccgctcgc
     1321 tcggccctcg gccgtggcgc gtcgcccatc ttcagcccgc gcgcaggccg gctggtggcc
     1381 gctgtgggca gaacccaggc cagatgcaac gctcctatca gtacgagggg caagagagtg
     1441 cgcaagcata gccccacctg gcgtgactgg ctacataccc tcgtagtggc aggcggtctc
     1501 ttcctcttca ccgagacctt cctgctgcag ggtttcagcg tgcatggaag ctgcatggag
     1561 ccgaacctct acaccggcga gcgcctcctg ggcaataagc tccgctaccg gctccacccg
     1621 ccgcagcgcg gcgacgtggt tgtcttccgc tatcctgaca acccccggaa gatctacgtc
     1681 aagcgcgtgg tcgccctgcc tggcgagatg atcgccatcc gcgacggcac cgtctacctc
     1741 aatggcaaac cgcttcatga gagctatgtg atcaacccgg cgcatggctc ctatccaccg
     1801 gctcgcgttc cctcggggca atacttcgtg ctcggcgata atcgtgacca cagcagcgac
     1861 tcgcgcgcct gggggttcgt gcccacgcgg cacctggagg ggaaggtgtg gctccgctac
     1921 tggccgctgc gccgggcagg catcttgccg gccggccggt cgtgcccttc ccacggagag
     1981 caatcgtgaa ggagctggtt ctggagatcg gcgtcgaaga tctacccccc gccgctatct
     2041 cccctgccct cgccgagatg aagcaactcc tcggcgagcg gctccggcag gagaatatcc
     2101 gctaccagga gatccgaacc tatggaacgc cccgccgcct ggtggcctgc gtcaccggcg
     2161 tcgcgccgcg ccagccggac acggtgatcg aggtgcgcgg gccctctgcc cagcaggcgt
     2221 tcgactcctt cggcaactac acctacgccg cgttcagttt cgcgcgcacc caggatgtct
     2281 tccccgaaga gctgaaggtg aagcggatca ccggtggtga gtaccttttc gtcgagcggc
     2341 ccaagccggg ccggccgacg gtcgaggtcg ccgcgggctt cctgccggag ctgatcacca
     2401 acctcgcctt tctcaggatg gcgcgccggg atgagagcgc gccgcgttcg cccatccgct
     2461 ggctcctcgc cctctacggg gaggatgtca ttccgttcca ggtgggagac gtggaagccg
     2521 gccggctcac gcgcggtcac cgcgcgctgc atggctgcac gccggagatc tcggccacca
     2581 tcgaggtgcc gaaagcctcc gcctatctgg agctcattcg cgcggccggg gtgatcctcg
     2641 atccggagga gcgccgccag cggatcctgg agcaggcgcg ggcgctcgcc caggcggtgg
     2701 gcggccagcc actcgaagag gcccttgcag acgagctcgc cttcctggtg gagcagcccg
     2761 cggcattcgc cggcggcttc gaccccgcgt tcctcgtgct tccccgcgag gcgctcgtcc
     2821 tcgtgatgca gaagcaacag cgcctcttcc caatcgtctc ccccgagggc gccctgctcc
     2881 cgcgcttcat cggcatccat gatggcagcc ccgagggcgt agaagaggcg cgtgccgcaa
     2941 gcgagcaggt gcttcacgcc cggctctctg accttcgcct ctcttacgag caagacctgc
     3001 gcacgccgct ccccgcgcgc gtggatgctc tccacagcat cccctatcag gagcggctcg
     3061 gcacgctctt cgagaagacc gagcgcctcg gctcgctcgt cgaggagcta tgtgaccggt
     3121 ggggcgtaga cgccaccacc cgccagaatg ccttgcgcgc ggcgcttctg gccaaggcgg
     3181 acctcgccac cgacttgctg aaggagctgc ccgaactgcg gggccggatc ggccaggcct
     3241 acgcgcttca cgccggcgag ccgccggcgg tagccgatgc acttgcgggc cactacctcc
     3301 cccgcgtagc cggcgacccg ctgccgcaga ccgaggcggg gcggatcctg ggcgtggccg
     3361 accggatcga caccctcgtg ggccacttcg gaatggggtt ggtccctatc gggtcgcagg
     3421 atcctcacgc gctacgccag caggcgagtg ggctggtcgc gatcctggcg gaatcggagc
     3481 gcctctcgct gggcgagatg gtagaactcg cctacggcct gtatggcgat cgcggcccgg
     3541 gctggcgaag tctcgatgta acccgcgcct ctttgctcga cttcctccgc caatgcctgg
     3601 agagccttct cagcgagcac ggcgtgcggt atgacaccgc cgatgccgtc ctggacgccg
     3661 gctttgatga cgtgcgtggc acacttatta gagcacagct cctcgaggag cgacgcgatg
     3721 ccccggagtt cgccgagact ctgatggccg cggcacgcct gggcagcctt ctgcgcttcg
     3781 tggcgcgctc ggagatggct gttcccccgg gagaagtacg catcgacctc ctccaggaag
     3841 aggcggagca gctcttctac gccgcgtacc tcgacgtctg ccctctggtg acggaagccg
     3901 cagagcgggg cgactatgcc gcggcgctcg atgccctggg cgggctcgcc gcgccggcgc
     3961 ttgagttctt cgataacgtg ctggtaacga gcgacgatga gctgctgcgg gccaaccggg
     4021 tgcgcctgtt gggcgagatt cacgcgcttt tcctgaagat agcggatctc acgcggctca
     4081 cggtcgaaat agaatagggt gctcgccttt ccggagcgaa agcggagagg gaagaactgg
     4141 ttgccctctc ctcgctgtgc catccaccag cggagtgtag gcagatgagg cggcgagagg
     4201 agacttgcag ggaggcagaa cgtcgggtgg agtttgagat caagcccttt gatgtatcgc
     4261 acaaagcaca gattctcggg ctttcgctgc gcgcctgggc cccagttttc tccgggttga
     4321 gaccggcggt gccagagtat gtctatgagg ccttctatcc tcatggctgg cgagcgaggc
     4381 agcgttcgga catcgaggct tttctggaca cggaggggga tcgcgtttgg gttgcggtca
     4441 aggagagggc cgtgctggga tgggttggca ttc
//
LOCUS       DAOSCV010000291         4473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12260_length_4473_cov_3.090991, whole genome shotgun sequence.
ACCESSION   DAOSCV010000291 DAOSCV010000000
VERSION     DAOSCV010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12260_length_4473_cov_3.090991"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..218
                     /locus_tag="PLY56_08295"
                     /pseudo
     CDS             1..218
                     /locus_tag="PLY56_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
     gene            367..597
                     /locus_tag="PLY56_08300"
     CDS             367..597
                     /locus_tag="PLY56_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21522.1"
                     /translation="MAQIQQPAQKVLFGDSDGNGYAAWRAAGDTYRYYFQSRVQAPHG
                     EGLNVCFYDGHVKWMKKDTLDDRRYWQYNYDF"
     gene            complement(670..1908)
                     /locus_tag="PLY56_08305"
     CDS             complement(670..1908)
                     /locus_tag="PLY56_08305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ21523.1"
                     /translation="MEAREALALHGGPKTKTTPYGTGVKHDATAEAEALRARLEAGPL
                     PLAMGPSIKELRQKVQEMFGVRFCVPTSSGTAAVHTALAALGVTPGDEVITTPVTDHG
                     TVIGIMQLNAIPVFADVNPATMMIDAGTIEPHITSRTRVILPVHLAGCPADMDGICRL
                     ARKHGLKVLEDCAQSWMAEWNGRLVGTIGDAGIFSINESKHISAGEGGVLLTNDEEVA
                     RYADLFADKSYNRSGQGPIDPVMPALNYRMSEVNAVLAIEQLKKVREVAARRWELGER
                     LAAEIEGLPGIRLLRPPAGSKSSYWHGVLLVDKEEAGVDGPEFTRALVAEGISASCPV
                     SRNLLDWPLFRRLNEDPRAFPTYIAPGLEKGRFALESCPNVLALATRSVRVHLNEFCT
                     DQDIDETARAIRKVARWYRG"
     gene            complement(1957..3081)
                     /locus_tag="PLY56_08310"
     CDS             complement(1957..3081)
                     /locus_tag="PLY56_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ21524.1"
                     /translation="MARIDASEVGGLQAALDLLPETGGTVYLPAGKYVITETVEKRLA
                     EGQHLFLVGEGRATVLVNENRDGAPLLRLVGAVGQWWPDLKITIRDLTFVGNYDSGDA
                     LVIDHPNDTMVDGCFFYGHGGHALYLKTQGTNVTCRDCWMRDCKRGVRAENIHHLTLH
                     GLQTRSRDGGQVQAEHLFLDRNCREVRVVNNHFAYGHAEAIILDGTAQHAIVGNTIEG
                     FQVGILARNDRERDCRDITLGSNYIHYGCAIHLEGACNGFVISDNIITDAHAGAVIIE
                     RAQESGAHAITGNVIRKSVYQGQGGIALGDSRGCSVVGNVFEEAHTMPAITAGPGGGE
                     HVIASNNITRAEGEQIVVKDAPGCLVSGNLVNGKRVDQIP"
     gene            complement(3081..4061)
                     /locus_tag="PLY56_08315"
     CDS             complement(3081..4061)
                     /locus_tag="PLY56_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ21525.1"
                     /translation="MSIPFLAKPHQTQPRILKWAAEHPELIRLDSVRQYVGFDVYAVT
                     VTDTSVPDEKKTCCLFTQPHAHEPATTAGMMDFLCQVIEGRHLDGSPADLPREEILRN
                     LLLSIIPDANPDGRSRSPELYWDGSKWSNDEFWYTMRGIDPRTLKMWERFDEWDLREQ
                     SPRPLTIGIVYEQVGPFEYVEPNRSHRSAYFRLIHHLLGQRRYHYLLDLHQTEFERSP
                     HNCMVILPILQPELPEAIRSTNQAWGEEIVAAWAASGASPVPEVAPLNYTGVQAEYFR
                     RSWRDIVRVTSKVTVEIQNNNQRTPPQEQVRLMDVAMRVTVERALRGGAA"
     gene            complement(4215..>4473)
                     /locus_tag="PLY56_08320"
     CDS             complement(4215..>4473)
                     /locus_tag="PLY56_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HOJ21526.1"
                     /translation="AVGVKPIVGSDPFVLVTTASHLRRAMALFEAQGLSPIPAPAEPR
                     VRYRPLPDLRWLYPSAENLQVSTEVVYEFLGMAWARLRGKA"
BASE COUNT          729 a         1501 c         1437 g          806 t
ORIGIN      
        1 gccgcgatcg ggcagcgatg cgcctcatga tcgagtgcat gatcgcgatc ggcgtcggct
       61 tcgtcacgct cgcgatcccg ctcgcgctgg acgtgaagtg gacgtcgtcg gcctgggcac
      121 tggagggcgc cggcgcattc tgggtcggca tgcggcaagc gcgctggatg ccacgcctct
      181 tcggcctgct gctccagttc gtggcggcct tcatcttttt cgtgtcgctc gacggcaacg
      241 tttcgagcat tcccttcgcg aaccccggct tcttccaccg acgaccgaat cctgcgcccg
      301 gcagcgccgc ggagccccgg ccggggcctc tggtattggg acaacatgga cctggatagc
      361 aagtcaatgg cccagatcca gcagccggcg cagaaggtcc tctttggcga ctcggacggc
      421 aacggctatg ccgcctggag ggcggccgga gatacctacc gctactactt ccagagccgg
      481 gttcaggcgc cacacggcga gggtctgaac gtctgcttct acgacggcca cgtgaagtgg
      541 atgaagaagg acaccctcga tgaccggcgc tactggcagt acaactacga cttctgacgc
      601 gtgacttcac agcacggggc gccaggctac gcctggcgcc ccgtattgct ttttccgagc
      661 tctgcggcct cagccgcggt accagcgcgc caccttgcgg atcgcgcgcg ccgtctcgtc
      721 gatatcctgg tcggtgcaga actcgttgag gtgcacgcgc acgctccgcg tcgccagcgc
      781 caggacgttg gggcagctct ccaacgcgaa tcgacccttc tccaggcccg gcgcgatgta
      841 ggtggggaag gcacgcgggt cctcgttcag cctgcggaag agcggccagt cgaggaggtt
      901 ccgggagacg gggcacgagg cactgatccc ctcggcgacg agggcgcgtg tgaactccgg
      961 gccgtccacc ccagcttctt ccttgtcgac caggagcacg ccatgccagt agctcgactt
     1021 gctccccgcc gggggccgca gcagccggat gcccggcagc ccctcgatct cggccgccag
     1081 gcgctcgccc agctcccagc gccgcgcggc cacctcacgc accttcttca gctgctcgat
     1141 tgccagaaca gcgttcacct cgctcatccg gtagttgagc gcgggcatca ccgggtcaat
     1201 gggcccctgc cccgagcggt tatagctctt atcggcgaag agatccgcgt agcgcgcgac
     1261 ctcctcatca ttcgtgagga ggacgccccc ctcgcccgcg ctgatatgct tcgactcgtt
     1321 gatggagaag atgccggcat ccccgatggt gcccaccagc cggccgttcc actccgccat
     1381 ccagctttgg gcgcagtcct ccagcacctt cagcccgtgc tttcgcgcca ggcggcagat
     1441 gccgtccata tccgccgggc agccagccag gtgcacggga aggatcaccc gggtgcgcga
     1501 ggtgatgtgc ggctcgatgg taccggcgtc gatcatcatc gtcgcggggt tcacatcggc
     1561 gaagacaggg atcgcgttga gctgcatgat cccgatcacg gtgccgtggt cagtgaccgg
     1621 cgttgtgatc acctcgtcgc cgggcgtcac gccgagggcg gccagcgcgg tgtggaccgc
     1681 cgcggtgccc gaggaggtgg gcacgcagaa gcggaccccg aacatctcct gcactttctg
     1741 gcgcaactcc ttaatggagg ggcccatggc aaggggaagt ggcccggcct ccagacgggc
     1801 gcgcagcgct tccgcctccg ccgtggcatc atgcttcacg cccgtgccgt agggcgtcgt
     1861 cttcgtcttc ggcccgccgt ggagggcgag cgcctctcgg gcttccatcc tgtatgtacc
     1921 tcctgtcctg acaaggggat aggccatgcc gatggactac ggtatctggt ccaccctctt
     1981 gccgttgacc agattcccgg aaaccaggca cccgggagca tccttcacca caatctgctc
     2041 gccttcggct cgggtgatgt tgttcgacgc gatgacgtgc tcgcctccgc cggggccggc
     2101 agtgatcgcc ggcatggtgt gcgcctcctc aaagacgttg ccgaccacgc tgcacccgcg
     2161 gctatcgccc agggcgatgc cgccctggcc ctggtaaacg ctcttgcgga tgacgttgcc
     2221 ggtgatggcg tgcgctccgc tctcctgcgc gcgctcgatg atgacagcgc ccgcgtgcgc
     2281 atcggtgatg atgttgtcgg agatcacaaa gccgttgcag gcaccctcca gatggatggc
     2341 gcagccgtag tggatgtagt tcgagccaag ggtgatgtct cggcagtcgc gctcgcggtc
     2401 gtttcgggcg agaatgccca cctggaagcc ctcaatcgta ttgccgacga tggcgtgctg
     2461 ggccgtgccg tcgaggataa tcgcctcggc gtgcccatag gcgaagtggt tgttcaccac
     2521 gcggacctcg cggcagttgc ggtcgaggaa gaggtgctcc gcctgcacct gcccgccatc
     2581 ccggctgcgc gtctgcaagc catgcagcgt caggtggtgg atattctcgg cgcgcacgcc
     2641 ccgcttgcaa tcgcgcatcc agcaatcgcg gcaggtgacg ttggtgccct gcgtcttcag
     2701 gtagagcgcg tgcccgccat ggccatagaa gaaacagccg tccaccatcg tgtcgttagg
     2761 atggtcgatg acaagagcat cgccgctgtc gtagttgccg acaaaggtga ggtcgcggat
     2821 ggtgatcttt aggtccggcc accactgccc gacggcaccc accaggcgca ggagaggcgc
     2881 gccatcccgg ttctcgttca ccagcacggt ggcgcggcct tcgcccacca ggaagagatg
     2941 ctggccctcg gccaggcgct tctccaccgt ctccgtaatc acatacttcc ccgccgggag
     3001 atagaccgtg ccgcctgtct ccggcagcag atccagggca gcctgcaacc cacccacttc
     3061 ggaagcatca atccgtgcca tcaggcggct cctccccgca aggcgcgctc caccgtcacg
     3121 cgcatcgcca cgtccatcag gcgcacctgt tcctgcggcg gcgtgcgctg gttgttgttc
     3181 tgtatctcga cggtgacctt ggatgtgacg cggacaatat ctcgccacga gcggcggaag
     3241 tactccgcct gcacaccggt gtagttcagg ggagccacct ccggaaccgg gctggcgccg
     3301 gaagccgccc atgccgccac gatctcctct ccccacgcct ggttggtgga gcggatcgcc
     3361 tcgggaagct cgggctgcag gatcgggagg atcaccatgc agttgtgcgg cgagcgctcg
     3421 aactccgtct ggtgcaggtc aaggagataa tggtagcgcc gctggcccag gaggtgatgg
     3481 atcagccgga agtaggccga gcggtgactg cggtttggct ccacgtattc aaacggccct
     3541 acctgctcgt agacgatccc gatggtgagc gggcgcggcg actgctcgcg caggtcccac
     3601 tcatcgaagc gctcccacat cttcagagtc cgcgggtcaa tccccctcat ggtgtaccag
     3661 aactcgtcgt tcgaccactt gctcccatcc caatagagct ctggcgagcg cgaccgccca
     3721 tccgggttgg catcggggat gatgctaagg agcaggttcc gcaggatctc ctcacgcggg
     3781 aggtccgcgg gcgagccatc gaggtgccgg ccttcgatca cctggcaaag aaagtccatc
     3841 atgccggcag tcgtcgccgg ttcgtgcgcg tgcggctgcg tgaagagaca gcacgtcttc
     3901 ttctcatcag gcaccgaagt gtcggttacc gtcaccgcgt acacgtcgaa cccgacgtac
     3961 tggcgcacgg aatcgagccg gataagctcc ggatgctccg ccgcccactt cagtatcctt
     4021 ggctgcgtct ggtgcggctt ggccaggaac gggatagaca taaggcctcc tcaggagagc
     4081 ggcgcgcccg gcaggcgagg gcgcgccagc gacttgtgat ccccttttcg cgatcgccgc
     4141 cccggtcgcc ttcttactgc agtgaaccaa tggtcctttt attaccggga tggctgttgc
     4201 gcgagcgctc cggctcatgc ctttccgcgg aggcgtgccc acgccatccc aaggaactca
     4261 tagacgacct cggtggaaac ctggagattc tcggcgctag ggtagagcca ccggaggtct
     4321 gggagggggc gatacctcac gcgcggctcc gcgggggccg ggatgggcga gaggccctgt
     4381 gcctcgaaga gcgccatcgc ccgtcgcaag tgcgatgcgg tcgtgaccaa gacgaaggga
     4441 tcgctgccga cgatcggctt caccccgacc gcc
//
LOCUS       DAOSCV010000292         4470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12269_length_4470_cov_3.586183, whole genome shotgun sequence.
ACCESSION   DAOSCV010000292 DAOSCV010000000
VERSION     DAOSCV010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12269_length_4470_cov_3.586183"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLY56_08325"
     CDS             <1..250
                     /locus_tag="PLY56_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21527.1"
                     /translation="PGPDESYTLITARVEVVEPEGEDRFEGSVRGWIRPAGELGAFLQ
                     RYSELGGTHHAALVYTDSSGSDEIAMFGALMGWKVVSL"
     gene            247..1749
                     /locus_tag="PLY56_08330"
     CDS             247..1749
                     /locus_tag="PLY56_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ21528.1"
                     /translation="MSAGHAPSPGGPAYLGLDVGGTGAKAGVFDAAGRRLGQGHVTYV
                     PSVSPEGHAEISIERIYEAARDAARQAVAEAGVPVAALAISSQGQTFVSLDASDRPLH
                     PAILWYDSRASKEAEELRARVASLGAPELARGITAIAVAPKIRWLRRYCPKAMERARR
                     YLLLPDYLNYRLTGEAVTDPSTASSTGLYAYDLPDYPPAALAAAEIDRAQVARIQPSG
                     TSVGRVRPEMAAEWGLSPETLVGVGTNDQYAGALGVGNCRPGILSETTGTCLALVTLA
                     RRLPEPMPPGLLGGRFPIPEYYFALAYSKTAGLVLEWFRRVLASGKSMAELDAMAAGV
                     LPGSRGVVMLHHFDGMVSPRPNPGARGAFLGLSLSHTAADLYRSILESIAFSLRENLE
                     LLGDVGLRVDVIRAIGGGARSDLWLQMKADLCGLPVERPEVTEAATLGSAMLAATGAG
                     RYASLAEASDRCYRAARTFAPNGRAQQQYQEAYAAYRDACERLHPSQRSG"
     gene            complement(1803..2069)
                     /locus_tag="PLY56_08335"
     CDS             complement(1803..2069)
                     /locus_tag="PLY56_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ21529.1"
                     /translation="MIFVRELGEDEHQELRRMTRQAVGRVSLRAHMILLSAQRRSVPE
                     IASLFDTSHATVRFWIERFHERGCHGLYDNPRSGRPRKSQLNTT"
     gene            2767..3408
                     /locus_tag="PLY56_08340"
     CDS             2767..3408
                     /locus_tag="PLY56_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOJ21530.1"
                     /translation="MRCLWKALFLGAGLLLPALAEATPSFLGPVGHVLTPSAETLGWR
                     RYAFTLHHQPHWNLLSASYSPLNSLEVGLTFVDPTRPGEYQTHAGANVKFRLLREQEG
                     LPAISVGVIDALGTLKEGPYRRSYYGVISRHFRVEGIQSPVQVTVGTGSGILKRGFFA
                     VAAPVHPMGTAILEYDGTHFNPCARLFLPRGFMLDVAAVDGQFGVGAAFRAEW"
     gene            3408..4391
                     /locus_tag="PLY56_08345"
     CDS             3408..4391
                     /locus_tag="PLY56_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21531.1"
                     /translation="MPDQPPSRSHPEIDLSPLNTYSIRERRSKVSVDSFATPLAPGAS
                     IGDLLSSLPRILAGNTFREAITALAGAVRSRRAVVLTMGAHVIKCGLAPILIDLMRRG
                     YLSAVATNGAGAIHDLEIALFGQTSEDVESELPAGRFGMARETAEFFNAAASEAAATR
                     TGLGATLGGRLLKAGAPHADVSLLAQAAAIGVPVTVHVALGTDIVHMHPSADGAALGD
                     ASLRDFRILANVLRGINHGGVVVNVGSAVVLPEVLLKCFSLLRNLGHDLSGCTGINLD
                     FIQQYRSGTQVVRRLELLGGRGLALTGHHELMVPLLAWAWTAEVEERRGSE"
BASE COUNT          642 a         1454 c         1578 g          796 t
ORIGIN      
        1 cccgggcccg gacgagagct acaccctgat caccgcgcgt gtcgaggtgg tcgagcccga
       61 gggggaggac cgcttcgagg gctcggtgcg cgggtggatc cggcccgccg gggagctcgg
      121 agcgttcctc cagcgctaca gcgagctggg tggcacacac cacgcggcgc tcgtctatac
      181 cgattcgagc ggcagcgacg agatcgccat gttcggcgcg ctgatgggct ggaaggtggt
      241 ctcgctgtga gtgccgggca cgcgccatcg cccggtggtc cggcctatct cgggctcgat
      301 gtgggcggca cgggcgccaa ggcgggggtg ttcgacgcgg ctggccgccg gctggggcag
      361 gggcatgtga cctacgtgcc atccgtctcg ccggaaggcc acgcggagat ctcgatcgag
      421 cggatctacg aggcggcgcg cgatgccgcg cggcaggcgg tcgcggaggc gggcgtgccg
      481 gtcgccgcgc tcgccatctc cagccagggg cagacgttcg tatcgctaga tgccagtgac
      541 cgcccgcttc acccggcgat cctgtggtac gacagccggg cctcgaagga agcggaggag
      601 ctgcgcgcgc gcgtggcctc tctgggagcg cccgagctgg cgaggggaat caccgcgatc
      661 gcggtggcgc cgaagatccg gtggctgcgc cgctattgtc ctaaggcgat ggagagggcc
      721 cggcgctacc tgctgctccc ggactatctg aactaccgtc tcaccggcga agcggtgacc
      781 gacccctcga cggcatcgag cacgggcctc tacgcctacg atttgccgga ctacccgccc
      841 gcggcgctcg ccgcggctga gatcgaccgg gcgcaggtgg cgcgcattca gccttctgga
      901 acctccgtgg ggcgcgtccg cccagagatg gccgctgagt ggggcctctc gcccgagacg
      961 ctggtggggg tgggaacgaa cgaccagtac gccggcgccc tgggcgtggg caactgccgc
     1021 ccggggatcc tctcggagac gaccggcacc tgccttgccc tggtgacgct cgcccggcgc
     1081 ctgccggagc cgatgccgcc gggactcctc ggcgggcgtt tcccgatccc cgagtactac
     1141 ttcgcgctcg cctactcgaa gacggccgga ctcgtgttgg agtggtttcg ccgcgtgctc
     1201 gcgtccggaa agagcatggc ggaactggat gcgatggcgg ccggcgtgct ccctggcagc
     1261 cggggcgtcg tcatgctcca ccatttcgat ggcatggtct cgccccgtcc aaacccggga
     1321 gcgcgcggcg cgttccttgg actctcactt tcgcacaccg ccgccgacct ctaccggtcg
     1381 atcctggagt cgattgcgtt cagcctgcgg gagaacctgg agcttttggg cgacgtgggc
     1441 ctgcgcgtag acgtgatccg agcgattggt ggaggggcgc gcagcgacct ctggctgcag
     1501 atgaaggccg atctgtgcgg gctcccggtg gagcggcccg aggtgaccga agccgcgact
     1561 ctcggctcgg cgatgctcgc ggcgaccggc gcgggccgct atgcgtcgct ggctgaggcg
     1621 agcgatcggt gctaccgcgc ggcacgaacc ttcgcgccga atgggagagc gcagcagcaa
     1681 taccaggagg cgtatgccgc ctatcgtgat gcctgcgaga ggctccatcc cagccagcgg
     1741 agcgggtgaa tgcgcggggg gcgcgagtgg cgacgcacgg gcctctcact cgcgcctctt
     1801 gctcacgtgg tgttgagctg gctcttgcgc ggccggccac tacgtgggtt gtcgtacagc
     1861 ccgtgacagc cacgttcgtg aaaacgctcg atccagaagc gcacggtggc atggctggta
     1921 tcgaagagcg acgcgatctc gggaacggaa cgccgctgtg cggagagaag gatcatgtgg
     1981 gcacggaggc tgacccggcc tacggcctgg cgtgtcatgc gtctcagttc ttgatgctcg
     2041 tcttcgccga gctccctcac gaagatcatg gtgcgcctcc catgcaggca ccgcgtgcgc
     2101 ggttatctct cccgccgctc tggctgctcg cggcgactgc ggccacggaa gcagtcgcca
     2161 ggcatgggag agacggcccg tatcctgccc gggtgctatc ggtgcaaatt gtgtccaggc
     2221 accgggcgcg tgccgggact ggtgtttttg tgcgtaagtt tcgattactt atacatattc
     2281 tccttcttcc cgcggatgaa gagattcttt cgtggcgcgt tttggccagc gcattggctt
     2341 gtgcgggcga taccgcacga gctcttagct ccgcgcctat gggagcggga gacacctgca
     2401 ctgcccagcc gcgctcgctc ccttcatccg tggcatggcc gcttgaagga ccgaatgctt
     2461 gaccgccggg tttccttgtg ctacaatcgg tgagataccg gaacgcccgg ggcctatgca
     2521 cgggtggtcg cctgggagag catcgaggcc cctcgcccgg cttccccgga gaagaattgc
     2581 agccgctcgc ccgcttcctg gtcgcgccat ggccggcggc ctccggatcc tctcgagccg
     2641 cgcctcggat cctgtgcccc ggggaaggcc cggcgcctgc ctctcctgca cccggaccgc
     2701 gtcttcatga gcatggcggc cgccaggtgg cgccggaacg gccgtgtggc ggaaggaggt
     2761 tgttctgtgc gttgcctttg gaaagccctc ttcctggggg caggcctgct cttgccagcc
     2821 ctggcggagg ccacgccctc gttcctgggg cccgtcgggc atgtgctcac gccatcggca
     2881 gagacgctgg ggtggcgccg ctatgccttc acgcttcacc accagcccca ttggaacctg
     2941 ctcagcgcaa gctacagccc gctgaacagc cttgaagtgg ggttgacttt cgtcgatccg
     3001 acgagaccgg gggagtatca gacacatgcc ggtgccaatg tgaagtttcg cctgctgcgc
     3061 gagcaggagg ggctgccagc catatcggtc ggggtgattg acgctttggg aacgctgaag
     3121 gaggggccct accggcgttc ctactacggt gtcattagca ggcatttccg cgtggagggc
     3181 atccagtctc cggttcaggt cactgtcgga accgggagcg gcatcctgaa gcgggggttc
     3241 ttcgccgtgg ctgcccccgt ccacccgatg ggcacggcga tcctcgagta cgacggcacg
     3301 catttcaacc cgtgcgcccg cctcttcctc ccgcgtggct tcatgctcga tgtggccgcg
     3361 gtcgacgggc agttcggcgt cggcgctgca ttcagagcgg agtggtaatg ccagatcagc
     3421 ctccttcccg gtctcacccg gagatcgacc tctcgccact caacacctat tctatccgcg
     3481 agcgtcggag caaggtgtcg gtggatagtt ttgccacgcc cctggcgccc ggagcgagca
     3541 taggcgacct cctctcatcg ctgccgcgga ttctcgcggg caacaccttt cgcgaggcca
     3601 tcaccgcgct tgccggggcc gtccggtcac gccgggcggt cgtcctcacg atgggcgctc
     3661 acgtgatcaa gtgtggtctg gcgcccattc tcatcgacct gatgcgccgg ggctacctgt
     3721 cggcggtggc gaccaacggc gccggcgcga ttcacgacct cgagatcgcc ctctttggcc
     3781 agacctccga ggacgtggag agcgaactgc cggcggggcg cttcgggatg gcgcgcgaga
     3841 ccgcggagtt tttcaacgcc gctgcctcgg aggccgccgc tacgcggacc ggcctgggcg
     3901 ccaccctggg tgggaggctc ctgaaggcgg gtgcgcctca cgcggatgta agccttctcg
     3961 cgcaggcagc ggcgatcggg gtgccggtga cggttcacgt cgccctgggt accgatatcg
     4021 tgcatatgca cccctcggcg gatggtgcgg cgttgggcga tgccagcctg cgcgatttcc
     4081 ggatcctggc gaacgtgctt cgcgggatca accacggggg cgtagtggtc aacgtgggat
     4141 ccgccgtggt cctcccggaa gtgctgctca agtgcttctc tctcctgcgc aacctggggc
     4201 acgacctctc cgggtgcacc ggaatcaacc tcgatttcat ccagcagtat cgctccggga
     4261 cgcaggtggt gcgccggctg gagttgctgg gcggtcgagg gctggcgctg acgggccacc
     4321 acgagctgat ggtgcccctt ctagcctggg cgtggacggc ggaggtggag gaacggcgtg
     4381 gaagtgagta gggcgcggct gcgtgcgctg gtcgagcgga ttcgcgggcg acgcacgctc
     4441 gtcatcggcg acgtgatgct cgacgagtac
//
LOCUS       DAOSCV010000293         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12291_length_4462_cov_3.799183, whole genome shotgun sequence.
ACCESSION   DAOSCV010000293 DAOSCV010000000
VERSION     DAOSCV010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12291_length_4462_cov_3.799183"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLY56_08350"
     CDS             complement(<1..1349)
                     /locus_tag="PLY56_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ21532.1"
                     /translation="MCGAWKGGDHGAAEAFQGEGFTILESDGSAEAKRAAFDRQIDEL
                     EAGENPPEVYVLVTHDPALAAVADRILAARRDVRVWGLACSAPEELRAEAFQFYSLED
                     LLDLRVRSHALFIDFENVAFCLHDLGYTVDPAVLARHFRERAAQGGGVLHAFAYADWH
                     RLPPMQDATGRSLSSEAQRVFELHNIETRYLISRAGKNTADMKIADDIRTLLHEPNSP
                     DTFIIASGDRDFTPVVDTLLRQGKEVIVWGVRGKTSRSLAEAAHVEYLQEFVPLASSP
                     PQPSDTTAAPTEGVNGLVGDTAPSIWTPLIVMLDETMSRNNWSWVSFSKLVSEANRSG
                     KFGASEEDTRDLINQAIARGVILRERIPNPNPNYPDQFIWACRPNDASPVVQAARIIP
                     PVTTEIMWRSIVERGWDYVAFSFLLREMANSSEIAQVNAAGDDDWRAAWINFLVSEG"
     gene            complement(2488..2694)
                     /locus_tag="PLY56_08355"
     CDS             complement(2488..2694)
                     /locus_tag="PLY56_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ21533.1"
                     /translation="MARGTVKWFDDAKGYGFITRPDGEDLFVHFSSIEGRGYRSLQEG
                     QSVDFDVERGPKGLQAVNVRPVSD"
     gene            3230..>4462
                     /locus_tag="PLY56_08360"
     CDS             3230..>4462
                     /locus_tag="PLY56_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21534.1"
                     /translation="MRWLLGVLLILSHAAVHANQAWELADPTEVRDPHNLLAVQVTGG
                     MLCGMTEWDPYFWLVLPEDGVDARLLSCVTMRLYSSAPADHVAVYYKSPNGEWGLGKS
                     LPVVEGWATYRLDMTEANWGESGGGAGARQWGGSTGKVISFRIDPGNEAGRWIMVDSV
                     RLHGKEAGFTPGVQAETAGAGVLEGLRVSPRVNAGQPIEVDATLRVERPGGGPTPAFI
                     WLFQGETLVRWRSLHAETGTGRVALREAFATSPYAFPAQFRVRVGIPGVTIPGVGLDS
                     EGTQVAFANPRAGKVKPPAVTVKRIGGDPAILLEGKPIAPFFVGSNDRDRIGRHKEFA
                     GYDLHLYSDWLAGSSTAGMLGQVKPGVYDYSVFDTYFAEVLEADPDAYFLPHVNLTAP
                     LWWQKAHPEELCAYADGGK"
BASE COUNT          788 a         1436 c         1477 g          761 t
ORIGIN      
        1 ttcccctccg agacgagaaa gttgatccat gccgcgcgcc agtcgtcatc gccagccgcg
       61 ttcacctggg cgatctccga cgagttggcc atctcacgca gcaggaagga gaacgcgacg
      121 taatcccagc cgcgctccac aatactgcgc cacatgatct cggtggtgac cggcgggatg
      181 atccgcgccg cctggacgac cgggctggca tcgttgggcc gacaggccca aatgaactgg
      241 tccgggtagt tggggttggg gttcgggatg cgctcgcgca gaatgacccc gcgtgcaatc
      301 gcctggttga tcagatcgcg cgtatcctcc tcgctcgcgc cgaacttccc gctgcggttt
      361 gcctcagaga cgagcttgct gaaggagacc cacgaccagt tgttgcgcga catcgtctcg
      421 tcgagcatca cgatcaacgg cgtccatatg gaaggcgccg tatcgcccac cagtccattc
      481 acgccctccg tgggcgcggc ggtcgtgtct gatggttgag gggggctcga tgcgaggggc
      541 acaaactcct gcaggtactc cacatgggcg gcttccgcca ggctccggct cgtcttgccg
      601 cgcactcccc aaacgatcac ctccttgccc tgacgcagca gcgtatccac cacgggggtg
      661 aaatcacggt cgccgctggc gatgatgaag gtgtcggggg agttcggttc atggaggagg
      721 gtgcggatgt catccgcgat cttcatgtcg gcggtgttct tgccggcgcg ggagatgagg
      781 tagcgcgtct cgatgttatg gagttcgaaa acacgctggg cctccgagct cagactgcgg
      841 cccgtggcat cctgcatggg agggaggcga tgccagtcgg cgtaggcgaa ggcgtgcaac
      901 accccgcctc cctgggccgc gcgctcgcgg aagtggcgcg ccagcacggc gggatcgacc
      961 gtgtagccca gatcgtgcag gcagaaagcc acattctcaa agtcgataaa gagcgcgtgc
     1021 gagcggacgc gcagatccag gaggtcctcc agcgagtaga actgaaacgc ctcggcgcgc
     1081 agctcttcgg gcgcggagca ggccaggccc caaacgcgca catcacggcg agcagccagg
     1141 atgcgatcgg ccactgccgc gagggccgga tcgtgcgtca ccaggacgta gacctccggg
     1201 ggattctcgc cggcctccag ttcatcgatc tggcggtcga acgctgcccg cttggcctcg
     1261 gcgctcccat cggactcgag gatggtgaag ccttcgccct ggaatgcttc tgcggcgccg
     1321 tggtcgcccc ccttccaggc accacacaca tatccgacga ggcctcccgt cagcaatctt
     1381 gcagaccgtc tcaggctttg agccaatacc gccggtgcaa ccggcaaaca cgctactcgt
     1441 agtgcttcat caatcccgtc aaaatcaacg aatagggccg taacgcgcat gctcctccct
     1501 ttccacacca caataaccag gccaatacac aaccggcgct cgaacacaac tccgccggtg
     1561 cggcagaaag cctgatcgcc aaatcacccc cggtcagcat ggggagtgca agcgatccca
     1621 aaacgctcat acgctgcacc taccccagaa gacggtcgcg aatgctatcg cggtttgccg
     1681 tgctcccttt ctctcccggg ctcagtatgg ctcgtcagag catctgtgcc ccgtaccgcg
     1741 gcatcgcaag cggcgtccgg tggacgaccg ccgctccacc agttcggaac gtggctacca
     1801 ctcagttcga cttcaggcac cgaatgccct ttcgcaaaga acacgccact tgcgggtgaa
     1861 gccatagctg caccgggcgg aagccagaat gcgccaaagc ccggatcccc cagccccgtg
     1921 cgaccgggcc ggcgattgct ggcctggcgc gaggtacttt cccgcgcgga tctcgggctg
     1981 ccgccatcag aacccgcccc tcttatctga aaaggcctcc ttacgtccct cgcgttacag
     2041 gacgacgagg ggcgtacgac gtcctgtgcg cgagtgccag gccggtgaag aggcgccaga
     2101 tggagaacgg ctcgcatcgg cggaggccgg gcgaccatcc cgaagtatgc accagaggtg
     2161 ccggccgggg gatggccacg gggctgctac ggtccgatcg aacggcgctc tctcgggcgg
     2221 cccgagcgtg agcgagcgcg gcctggcagg tggccgtggg cggcgtgcct gcgcgagcaa
     2281 gcaagatgct ccgaaaggat ggcgacactt gggaaagacg tgccggtcac tggagatccc
     2341 gttgaggatg cgccgtgccg ggagagtggc gtgtgtggcc gagagctgtg tggaacgccg
     2401 caaccgaggc ccgcaggcca gtggagtggg ccccccgggg tgtgaaccga acggtaagcc
     2461 tgctctccac tggcctgcta tcgactgtca gtcggagacc gggcgaacgt tgacggcctg
     2521 caagcccttg ggaccgcgct cgacgtcaaa gtcaacgctc tgcccctcct gcaggctgcg
     2581 atagccgcga ccctcgatgg atgagaagtg gacgaacagg tcctccccat ccggccgggt
     2641 gataaacccg taccccttcg cgtcatcaaa ccacttgacc gtgcctctgg ccattcctga
     2701 gaaacctcct atgctcccta tcgctggacg cctgatgctg gcggtcggag gggttcggaa
     2761 acagggccgc tcttgaagcg gccggcctcc gatatcccca gggagggcga tcccctcttt
     2821 tccagagccg gcggcatcct gacagggcag ccgggctcgc ggaccgtcgc aacttcgccg
     2881 taggtgcgcc caagccagta tacccgctgc cccgtcccag caaaccagga gagaacctcg
     2941 caaccgtggc tggatgaggt agttcccggg cgaggcggtc gggtgtcaac cctcgcacct
     3001 gttgataccg ataggcttcg cgcgggctag attcgggcgg catcatggca ggccggggcg
     3061 aaggattccc cccgtggctc ccgaagtcgt ggttcatgag gcgggcaggc gctgccatat
     3121 caaggtggcg atggcctgaa ggggcggggc gagggaatgc gcggattgcg gcccgcgttc
     3181 cgtttccacc gttgagtctg gcatctcgcc tggcgggagg gtgttcgcga tgaggtggtt
     3241 gctcggtgtt ctcctgatcc tctcacacgc cgcggtgcac gcgaatcagg cgtgggagtt
     3301 ggcggatccg accgaggtcc gggatccgca caacctcctc gcggtacagg tgaccggcgg
     3361 catgttgtgc ggcatgacgg aatgggaccc ctacttctgg ctggtgctgc cagaggatgg
     3421 ggtggacgca cgcttgttat cgtgcgtgac gatgcgcctc tactcctccg cgcctgcgga
     3481 ccacgtggcc gtctactaca aatctcccaa tggcgagtgg ggcctcggca agtcgcttcc
     3541 cgtcgtggaa gggtgggcga cttaccgtct cgatatgacc gaggccaact ggggagagag
     3601 cgggggcggc gccggtgccc ggcaatgggg cgggagcacc gggaaggtca tctccttccg
     3661 aatcgatccg ggcaacgagg ccggccgctg gatcatggtc gattccgtgc gcctgcatgg
     3721 caaggaagcg gggtttaccc ccggagttca ggctgagacc gcgggggctg gcgtcctgga
     3781 ggggcttcgt gtatcgccac gggtgaatgc gggccagccg attgaagtgg acgccacgtt
     3841 gcgcgtggag cgcccgggag ggggcccgac ccccgccttc atctggcttt tccagggcga
     3901 gacgctggta cggtggcgca gcctgcacgc cgaaacggga acaggccgcg tggcgctccg
     3961 cgaggcgttt gccacctcgc cctatgcctt tcccgcgcag tttcgggtgc gcgtgggcat
     4021 cccgggagtg acgatcccag gcgttgggct cgattccgag ggaacgcagg tggccttcgc
     4081 caatccgcgc gccggcaagg tcaagccgcc cgcggtcacc gtgaagcgca tcgggggaga
     4141 cccggccatt ctcctcgaag gcaagcctat cgcgcccttc ttcgtcggtt cgaacgaccg
     4201 cgaccgtatc ggccggcaca aggagtttgc tggctacgac ctccacctct actcagactg
     4261 gctggcagga tcgagcaccg cgggaatgct ggggcaggtg aaacccggcg tttacgacta
     4321 cagcgtcttc gacacgtact tcgccgaggt tctggaagcc gacccggacg cctatttcct
     4381 gccgcatgtg aacctcacgg cgcccctgtg gtggcagaag gcgcatcccg aggagctgtg
     4441 cgcctacgcg gatggcggga aa
//
LOCUS       DAOSCV010000294         4454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12323_length_4454_cov_3.484656, whole genome shotgun sequence.
ACCESSION   DAOSCV010000294 DAOSCV010000000
VERSION     DAOSCV010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_4454_cov_3.484656"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1437)
                     /gene="ffh"
                     /locus_tag="PLY56_08365"
     CDS             complement(103..1437)
                     /gene="ffh"
                     /locus_tag="PLY56_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ21535.1"
                     /translation="MFESLTDRLQGIFKGLRNKGKLTEQDVNDALRQVRLALLEADVN
                     FKVVKDLIAAIKERAIGQEVLQSLTPEQHVIKIVHEELTKLLGSTETRIQIASKPPTV
                     IMLVGLQGSGKTTQAGKLALHLRKQGKRPLLAALDIHRPAAIKQLQVVGAGLDIPVFT
                     LGEQQPAQIARAAVSYAESHGHDVVILDTAGRLHVDDEMMHEAKQIADAVTIHETLLV
                     VDAMTGQDAVNIAEQFSAALPVDGFILTKLDSDTRGGAAISIRAVTGRPIKFVGIGEK
                     MDALETFHPDRMANRILGMGDMLSLIERASQAVSKERERELEERLRSKDFNLEDYLEQ
                     LAAVKQMGPLDQVLGMIPGLSGLMSRRQVQVDENHLKRVEAIIQSMTVQERRHPHIIN
                     GSRRRRIARGSGTAVQDVNQMLNQFEQMKKMLRQFNEMESGRKRPTRLPFSF"
     gene            1740..3182
                     /locus_tag="PLY56_08370"
     CDS             1740..3182
                     /locus_tag="PLY56_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOJ21536.1"
                     /translation="MAYRDLKEFVKILRERGELKEIQTEVDPHLEITEITDRVVKRQG
                     PALLFTNVKGHRWPVLTNAFGTMERMALALGAESIDALAAEIAELLEPRVPATLVGKL
                     QMLPRLKVLADMAARTVASGPCQEVVITDSPSLDLLPIIQCWPLDGGPYITLPVVITK
                     HPERGTRNVGMYRLQKFDARTLGMHWQRHKGGAEHYRVAEALGRRLEVAIALGPDPAV
                     TYAATAPLPDEMDELMLAGYLRKRPVELVKCRTIDLEVPANAQIVLEGYVEPGERRRE
                     GPFGDHTGFYSLADDYPVFHLTAITHQRDPIYPTTVVGVPPMEDGPIGKATERLFLPL
                     IRKTVPEIVDLNLPVEGAFHNLAIVSIEKRYPGHARKVMHAIWGLGQLMTTKIVVVVD
                     KECNVHDIGEVIWRVGANLDPRRDVVLSEGPTDVLDHASQMPDWGGKLGLDATRKWPE
                     EGHTREWPPEIVMSPEVKARVDAIWPELGL"
     gene            3563..4078
                     /locus_tag="PLY56_08375"
     CDS             3563..4078
                     /locus_tag="PLY56_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21537.1"
                     /translation="MRDENESAARDQPGKDEENALKQLLEGRPGFEQRVGDYTIAVAT
                     GGARGAWVWHAGALHWRKPSTGENQHVDVVVRDAADGRFIPGLSVYVTLSTPGGQELG
                     TKVQPFLWHPFLYHYGANWCIPEEGDYNVTVRVEPATFPRHGKEMGERYTLEAVALFT
                     GLHMSPAVEEE"
BASE COUNT          773 a         1414 c         1429 g          838 t
ORIGIN      
        1 tgcccatgcg ccggagcctg atcttaactg ccaagagtgg ttccctcctc ggtgtgtttc
       61 gaggtcgccc cgcgcccgaa tagctggcgc ggtcgcctct cctcagaatg aaaacggcaa
      121 acgtgtcggt cgcttgcgcc cgctctccat ttcgttgaac tggcgcagca ttttcttcat
      181 ctgctcgaac tggttcagca tctgattgac atcctgcacg gcggttccgc tgccgcgcgc
      241 gatccgccgc cggcggctcc cgttgatgat gtggggatgc cgtcgttcct gaaccgtcat
      301 cgattggatg atcgcctcta cccgtttcag gtggttttcg tccacctgaa cctgacgccg
      361 actcatcagg ccgctgaggc ccggtatcat cccgagcacc tggtccaatg ggcccatctg
      421 tttgacggcg gcaagctgct cgaggtagtc ctcgagattg aagtccttgg agcgaagccg
      481 ctcctctagt tcacgctctc gctccttgct cacggcttgc gacgcgcgct cgatcagcga
      541 gagcatgtcg cccatgccca ggatgcggtt ggccatccgg tctggatgga acgtctccag
      601 ggcatccatc ttctcgccga tgccaacaaa cttgatgggc cggcctgtca ccgcgcggat
      661 cgaaatcgcc gcgccgccgc gcgtatcgct gtccagcttc gtgaggatga agccatccac
      721 cggcagcgcc gcgctgaact gctcggcgat gttcaccgcg tcctgacccg tcatcgcatc
      781 gactacgagg agcgtctcgt ggatcgtcac ggcgtccgcg atctgcttcg cctcgtgcat
      841 catctcatca tccacgtgca ggcgccctgc cgtgtcgagg atgacgacgt cgtggccgtg
      901 cgattcggca tagctgaccg ccgcgcgcgc gatctgcgcc ggctgctgct cgcccagcgt
      961 gaagacgggg atgtcgagac cggcgcccac cacctgcaac tgcttgatgg ccgccgggcg
     1021 gtggatatcg agggcggcaa gcagcgggcg ctttccctgc ttgcgcaggt ggagcgcaag
     1081 cttgccagcc tgggtcgtct ttccacttcc ctgcaggccc accagcatga tcacggtggg
     1141 gggcttcgag gcaatctgga tgcgcgtctc ggtgctgccc aggagcttcg tcagctcttc
     1201 atggacgatc ttgatgacgt gctgctccgg ggtgagactc tgcaacacct cttgcccgat
     1261 ggcccgctcc ttgatggccg cgatcagatc cttgaccacc ttgaagttga cgtccgcctc
     1321 gagcaaggcg agccgaacct gccggagcgc gtcgttgacg tcttgctccg tcagcttccc
     1381 cttattgcgc aggcccttga agatgccctg taacctatct gtcagactct caaacatagc
     1441 gacattctcg acgtaccgga caagtatagc acaggtgacg aacccaagtc aatgcaagat
     1501 tcgtgcgtga gatggatggc gctgctctcc gcgagagcgc tctccacgtc ctgcgcctcc
     1561 tgatggcagg attccgccgg gcaggagccc gaataggagc aaggctgcct gcgcatccgc
     1621 gcgtttgccg ctgccaccaa gcgatgccca cagcgcgcag aacggcgatg aaggagttct
     1681 ctgccaacgc tgggcggtag ccgcgtggca ttgcgggatg agggaatctg gaatcaggta
     1741 tggcgtatcg cgacctcaag gagtttgtga aaatcctccg ggaacggggg gagttgaaag
     1801 agatccagac cgaggtagat ccccacctcg agattaccga gatcaccgac cgtgtcgtga
     1861 agcggcaggg gcccgcgctc ctcttcacca acgtgaaggg ccaccgctgg ccggtgctga
     1921 ccaacgcctt cggcacgatg gagcggatgg cccttgcgct tggtgctgag agcatcgacg
     1981 cgctggccgc cgagattgcc gagctgctgg agccgagggt tcccgcgacg ctcgtgggca
     2041 agctgcagat gctcccgcgc ctcaaggtgc tggcggatat ggcggcgcgc accgtcgcgt
     2101 ctggaccgtg ccaggaagtg gtgatcaccg attccccctc gcttgacctg cttccgatta
     2161 tccagtgctg gccgctggat ggcggcccct atatcaccct tcccgtggtg atcacgaagc
     2221 atccggagcg agggacgcgc aatgtcggga tgtaccggct ccagaagttt gatgcgcgta
     2281 ccctgggaat gcactggcag cgccataagg gcggcgccga gcattaccgc gttgccgagg
     2341 cgcttggccg ccgcctggaa gtggccattg ccctcggacc cgacccggcg gtgacctacg
     2401 cggccacggc gcctcttccc gacgagatgg atgagctgat gctggctggc tacctgcgca
     2461 agcggcccgt ggagctggtg aagtgccgca ccatcgacct ggaggtgccc gcgaacgcgc
     2521 agattgtcct ggagggctat gtggagccgg gagagcgccg gcgcgagggc ccgttcggcg
     2581 accacaccgg cttctactcg ctggcagacg actaccccgt tttccacctc acggcgatca
     2641 cgcaccagcg cgatccaatc tacccgacca ccgtggtggg cgttccgccg atggaggatg
     2701 gcccgattgg gaaggcgacg gagcgcctct tcctcccgct gatccgtaag accgtacccg
     2761 aaatcgtcga cctcaacctg ccggtggagg gcgccttcca caacctggca atcgtctcca
     2821 tcgagaagcg ctatccgggg cacgcgcgca aggtgatgca tgccatctgg ggtttgggcc
     2881 agcttatgac aacgaagatc gtcgtcgtcg tagacaaaga gtgcaacgtc cacgacatcg
     2941 gcgaggtgat ctggcgcgtg ggcgccaacc tcgatcctcg gcgcgatgtg gtcctctccg
     3001 aggggcccac cgatgtcttg gatcacgcct cgcagatgcc ggactggggc ggcaagctcg
     3061 gcctcgatgc aacgcggaag tggccggagg aagggcacac ccgggaatgg ccaccggaga
     3121 tcgtcatgtc gccggaagtg aaggcgcgcg tggatgcgat ctggccagag ttggggctgt
     3181 agtctgtcgc gcctcttaaa aagagtggag gcggagggtg cgggcgcgcc tccgcctcct
     3241 gtagaggaac agggccagta gttcctccca ccactttcaa ttatcggctt gttcaaagcg
     3301 ccttccatgc gccaatcggc ctgctcgaca cggggcgcca gtcctaccgg ggtagctact
     3361 ccatcggcgt atatatccca cccgcgaggg atgcaagtgt atgctcccag tgctcgctct
     3421 gcttcgtccc taacgagggg tatctccctc acgtccttgc ccgctctgtt tgccgctgcc
     3481 tggcttgggg tacactacaa ccaggcggcc tccagcgggg tgggggcatc aggctagatg
     3541 ccgcctggta gggagatgac gaatgcggga tgagaacgag agcgctgccc gagatcaacc
     3601 cggcaaggac gaggaaaacg ccctgaagca gttgctggag gggcgcccgg gcttcgagca
     3661 gcgcgtgggc gactacacga tcgccgtcgc tacaggaggc gcgcgggggg catgggtgtg
     3721 gcatgcgggc gctctccact ggcggaagcc ctctacgggc gagaatcagc acgtggatgt
     3781 cgtggtgcgc gacgcggcgg acggccgttt catccctggc ctgagcgtct atgtgacgct
     3841 ctccacgcct ggggggcaag agctgggcac caaggtgcag cctttccttt ggcacccttt
     3901 cctctaccac tacggcgcca actggtgcat tccggaggag ggcgactaca acgtcaccgt
     3961 gcgcgtcgag ccggccacct tcccgcgaca cgggaaggag atgggcgagc ggtataccct
     4021 cgaagccgtg gccctcttca ccggcctcca catgagcccc gcggttgaag aggagtagtc
     4081 ccgtcgccgg gtggtccggc gtcgatacgg cggacgaagg agtcgggcac ctcaggctcg
     4141 gtagtgcgcg ccctttgggg ttgagactgg gccaccgcgt ggcgctggca gaggacattg
     4201 aaagggtccg ctattcgagc ccgatggttc cgcaccccgg gagccggcca cccagccggg
     4261 caagctatta gagagcatcc ctgtcggttc tggcatgacc gacgcggcat gaccaaaggc
     4321 ccttcttcag gacagagtct ccgctttgtg ttataatagg gtatcggcgt cctacgccag
     4381 ggcgtcctaa tccgacgatc ggcaatctgc gatccgcgat tgccggagga gtgaccatgc
     4441 gcagcgacac gatc
//
LOCUS       DAOSCV010000295         4443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12365_length_4443_cov_3.410893, whole genome shotgun sequence.
ACCESSION   DAOSCV010000295 DAOSCV010000000
VERSION     DAOSCV010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12365_length_4443_cov_3.410893"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLY56_08380"
     CDS             <1..443
                     /locus_tag="PLY56_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ21538.1"
                     /translation="LQWARGITFTGNTFFNGQRHSIHARQSTHLLVGSCVFDHNPDYP
                     RDAQDGLLFEECEGVTLTGLHLVDLLAGSPERGGAIELRACRGVSITDCQILNPAHRG
                     IALERCDRVLVANCLIADTRAQPSMVEAIRDEGSTRACFTGNLS"
     gene            complement(449..1684)
                     /locus_tag="PLY56_08385"
     CDS             complement(449..1684)
                     /locus_tag="PLY56_08385"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ21539.1"
                     /translation="MEGLELELWEVRQHLDTREIADVAGATRAALEGLATSHPWPRGG
                     EVAITAGSRGIDRIVDVLRAAAEYARARGCRPFILGAMGSHGGSTAAGQMEVLRGYGI
                     TEEGMDCPVRTCVESVEIGRTPEGLPVLCNRLALEADGVLLVNRVKPHTILTGDLGSG
                     LMKMAAIGLGGPRGADTIHARGLPECIVPATRVLLERLPILGGIGIVENAADRVCVLE
                     AVPPSAIEATDRRLLALARSILPSIPFDPIHVLVTRWMGKNLSGTGMDPNVIGMHRRL
                     GGPPERRIDRIVALDLTPESHGNAIGVGMADVITMRLRNQIDWQATYANGLTSGFFAG
                     IKLPVALPTDREAIATALKGFPRETVRLVIIRDTAHLERMLVSPALLEEARAHPRLSI
                     AETPTPLRFGEAGELLSVP"
     gene            complement(1755..2957)
                     /locus_tag="PLY56_08390"
     CDS             complement(1755..2957)
                     /locus_tag="PLY56_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ21540.1"
                     /translation="MEFEKSYDLLVAGAGVAGCAAALEAARAGLKVALLEKTVFTGGL
                     ATTGLINIYLPLCDGYGRQVLFGMAEEFLHESARYGPGDVPAQWRQSPVDGKHYRYRL
                     TFSSASFTLALDELLMKAGVDLWLDTLVCLPILDGNRVVGVEVENKSGRGAVYAKCVI
                     DATGDADVAYRAGCECAYGENFTSIWIFQASLATARQVAESGDASALLDGVRLRSQPG
                     QASIDPERRRWDATDGKDVTAYVQETYHLLREHYAKLHAEGGEKDRHNLYPITLPAMA
                     QFRRTRRIVGQATLSDGQDGQRFEDSIGISPDWRKPGPIWEIPYGTLVPRGRDAILAA
                     GRCIDSEGDAWEVLRVIPPAAHTGQVAGLAATLAIQNGTVPREVPVDAIQKALREKGI
                     PLHIDEVL"
     gene            complement(2999..3997)
                     /locus_tag="PLY56_08395"
     CDS             complement(2999..3997)
                     /locus_tag="PLY56_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ21541.1"
                     /translation="MLENEQEFIAQEWPFQPGERRVKMHLELPAAGISANTGLMLVLH
                     NWGGRYNEPHYVAWCRRFADRYNVIAVSVNYLQSGGGEPVIPGEKPYDHGYLQAIDCL
                     RALYHIRRQLQDAGVKFNPRRCYSMGGSGGGNVSLMVNKFAPHTFACVVDICGMPGLT
                     DGIAYGTGECGSQLNAGYSRDPSSPAYLSPARQELLDPGHPSHLVLQYAANPANKVVI
                     VHGLDDTSCPAIHKITIFRNMVAAGFRPDAHFLTPWHVDGEAVTTTGHPVGNRDRVVE
                     RFADCYLREEGEAALQIPGPEDFERASQVIYPVTGGRYVIDYSQGPPSIWFEEAEE"
     gene            4259..>4443
                     /locus_tag="PLY56_08400"
     CDS             4259..>4443
                     /locus_tag="PLY56_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ21542.1"
                     /translation="MPPVGALGAEERPRLGLALSGGAALGLAHVGALVWLEENRVPVD
                     YVAGASMGGLVGGLYAMG"
BASE COUNT          655 a         1520 c         1525 g          743 t
ORIGIN      
        1 acctccagtg ggcgcgcggc atcaccttca ctggaaacac cttcttcaac gggcagcggc
       61 actctatcca cgcccgccag tccacccacc tcctcgtcgg ctcctgcgtc ttcgaccaca
      121 accctgacta cccgcgcgac gcgcaggatg gcctgctgtt cgaggagtgc gagggcgtga
      181 ccctaaccgg cctccacctg gtcgatctcc tcgccggctc gccggaacgc ggaggcgcca
      241 tcgagctgcg cgcctgccgg ggggtctcca tcaccgactg ccagattctg aacccggccc
      301 atcgcggcat tgccctggag aggtgtgatc gcgtcctcgt cgccaactgc ctcatcgccg
      361 acacccgggc acagcccagc atggtggagg cgatccgcga cgaggggagc acccgcgcct
      421 gcttcaccgg caacctttcg tagccgggtc agggcaccga caacagctct cccgcctcgc
      481 cgaaccggag cggcgtaggc gtctcggcga tgctcagtcg cgggtgggcg cgtgcctcct
      541 ccaggagggc gggagagacg agcattcgct ccaggtgtgc cgtgtcgcgg atgataacca
      601 ggcgcaccgt ctcgcgcggg aagcctttca gcgcggtggc gatggcctcg cggtcggtgg
      661 ggagggccac ggggagcttg atcccggcga agaacccgga agtgagcccg ttcgcgtagg
      721 tggcctgcca gtcgatctgg ttgcgcaggc gcatggtgat gacgtcggcc atgccgacgc
      781 cgatggcgtt gccgtggctt tcgggcgtga ggtccagggc gacgatccgg tcaatccggc
      841 gctcgggcgg gcctcctagc cggcggtgca tgccgatcac gttggggtcc atgccggtgc
      901 cggagaggtt tttccccatc cagcgcgtca ccaggacgtg gatcgggtcg aacgggatcg
      961 atgggagaat cgagcgcgcg agcgccagca accggcggtc tgtcgcttca atggcgctgg
     1021 gcggcaccgc ctcgaggacg caaacgcggt cggccgcgtt ctcgacgatt ccgatcccgc
     1081 ccaggatggg gagacgctcc aggaggacgc gcgtggctgg cacgatgcac tccgggaggc
     1141 cgcgcgcgtg gatcgtatcg gcgccgcgcg gccctcccag gccgatggcc gccatcttca
     1201 tcagcccgct cccgaggtcg ccggtgagga tcgtgtgcgg cttcacgcgg ttgacgagga
     1261 gaacgccatc cgcctcgagt gccagccggt tgcagagcac gggcaggccc tccggcgttc
     1321 ggcctatctc cacggattcc acgcaggtgc gtacagggca gtccatgccc tcctcggtga
     1381 tcccgtagcc gcgcaggacc tccatctgtc cggcagcggt tgaaccgccg tgactgccca
     1441 tggcgcccag gatgaacggc ctgcacccgc gagcgcgcgc gtattccgcg gcggcgcgca
     1501 ggacgtccac gatccggtca atcccccggc tgccggcggt gatcgcgacc tcgccaccac
     1561 gtggccaggg gtgcgaggtc gcgagcccct ccagcgcggc gcgcgtagcc cccgcgacgt
     1621 cggcgatctc gcgcgtgtcg aggtgctggc ggacctccca cagctccagt tcgagcccct
     1681 ccacgccggc gccccccacc gcctgggtgg gtgagaacgt tgggcctggg gcttgcgccc
     1741 cggcggcctg cgacttacag cacttcatcg atgtgcagcg ggattccctt ctcgcggagc
     1801 gccttctgga tcgcgtccac cggcacttcg cggggcacgg tgccgttttg aatcgcgagt
     1861 gtggccgcga gcccggccac ctgcccggtg tgcgccgcgg gcgggatcac gcgcaggacc
     1921 tcccaggcgt cgccctcgct gtcgatgcac cgtccggccg cgaggatggc atcgcgcccg
     1981 cgcggcacca gggtgccgta cgggatctcc cagatggggc cgggcttgcg ccagtccggc
     2041 gagattccaa tcgagtcctc aaaccgctgg ccgtcctgac catccgagag cgtggcctgg
     2101 cccacgattc gccgggtccg gcggaactgc gccatcgccg ggagggtgat cgggtagagg
     2161 ttatgccggt ccttctctcc accttccgcg tggagctttg cgtagtgctc gcggaggagg
     2221 tggtacgtct cctggacgta ggcggtcacg tctttcccgt cggtggcatc ccagcgccgg
     2281 cgctccggat cgatgctggc ctgcccgggc tggctgcgca ggcgcacgcc gtcgagcagg
     2341 gcagaggcat cgccggattc ggccacctgg cgcgccgtgg cgagcgacgc ctggaagatc
     2401 cagatgctgg tgaagttctc gccgtaggcg cactcgcacc cggcgcggta ggcgacgtcg
     2461 gcatcgcccg tggcgtcgat gacgcacttc gcgtaaacgg cgccgcgccc gctcttgttc
     2521 tccacctcaa cgccaacgac gcggtttcca tccagaatgg ggaggcagac aagcgtgtcg
     2581 agccacaggt cgacccccgc cttcatcagg agctcgtcca gggcgagggt gaacgacgcg
     2641 gaagagaaag tgaggcggta gcgatagtgc ttgccatcca cgggcgactg ccgccactgc
     2701 gcgggaacat cgcccgggcc gtagcgcgcg ctctcgtgga ggaactcctc ggccataccg
     2761 aagaggacct ggcggccgta gccgtcgcag agcgggaggt aaatgttgat caggccggtc
     2821 gtcgccaggc cgcctgtgaa gaccgtcttc tccagaagcg ccactttcag gccagcgcgc
     2881 gccgcctcca gcgcggcggc gcacccggcc acaccagcgc cggcgactag gagatcgtag
     2941 ctcttctcga actccatttg gctcacactc caatcgcaac gtgcccctcg ggcggggctt
     3001 attcctccgc ctcctcaaac cagatggacg gcgggccctg cgagtaatcg atgacataac
     3061 gcccgccagt cacggggtag atcacctggc tggcgcgctc gaagtcctct gggcctggga
     3121 tttgcagcgc ggcctctccc tcctcgcgca ggtaacaatc ggcgaaacgt tccaccaccc
     3181 ggtcgcgatt gcccacggga tgaccggtgg tcgtcaccgc ctcgccatcc acgtgccacg
     3241 gcgtcaggaa gtgcgcatcc gggcggaagc cggcggcgac catgttgcgg aagatggtga
     3301 tcttgtgtat cgcagggcag gaggtgtcat ccaggccgtg gacgatcacc accttgttcg
     3361 ccggattcgc cgcgtactgc agcaccaggt ggctcggatg accggggtcg aggagctcct
     3421 ggcgggcggg cgagaggtag gccgggctgc tggggtcgcg cgagtagccg gcgttcagct
     3481 ggctgccgca ttccccggtg ccatacgcga tgccgtcggt cagcccgggc atgccacaga
     3541 tatcgaccac gcaggcgaag gtgtggggcg cgaacttgtt caccatcagg ctgacgtttc
     3601 caccgccgct tccgcccatc gagtagcacc gcctggggtt gaacttcacc ccggcatcct
     3661 gaagctggcg tcggatgtgg tagagggcgc gcaggcaatc gatcgcctgc aggtagccgt
     3721 ggtcgtacgg cttctctcca gggataaccg gctcgccgcc gccgctttgg aggtagttga
     3781 cgctcaccgc gatgacgttg tagcggtcgg caaaccgccg gcaccacgcc acgtagtggg
     3841 gctcgttgta gcgcccgccc cagttgtgga gcacgagcat cagccccgtg ttggcgctga
     3901 ttcccgccgc gggaagctcc agatgcatct tcaccctgcg ctcgcccggt tggaagggcc
     3961 actcttgcgc gataaactcc tgctcgttct ccagcactcg cgttctccct gccggcgcct
     4021 ggccggcgtg ccatgcctgc cggctcttct gccgtcaccg agatgctttc gccgctttgg
     4081 acgcggaaac ctgcacgaga ccggcccggc gcgtggccct gccgcgcccg gcgcgccctg
     4141 ttctccctcg cccgtgtgat cttccagccg aacgggtatg attacagcgg ggtagaggga
     4201 gagggatttg cggcattggc gatgcttcct ggtcggagtg ctggtggctg ctggcctcat
     4261 gccaccagtg ggcgcgctcg gtgcagagga gcgcccgcgg ctgggactcg ccctcagtgg
     4321 aggggccgcc ttgggattgg cgcatgttgg agccctcgtg tggctggagg agaaccgcgt
     4381 gccggttgac tacgtggccg gcgcgagcat gggcgggctg gtcggcggcc tctacgccat
     4441 ggg
//
LOCUS       DAOSCV010000296         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12394_length_4434_cov_4.127198, whole genome shotgun sequence.
ACCESSION   DAOSCV010000296 DAOSCV010000000
VERSION     DAOSCV010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12394_length_4434_cov_4.127198"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLY56_08405"
     CDS             <1..233
                     /locus_tag="PLY56_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ21543.1"
                     /translation="SEKAESAAKTVEKVNPAYTSQNCSACGERVLKALSVRWHSCPYC
                     GAELHRDHNAALNIRKKGGGTALVDVAALAAA"
     gene            409..2427
                     /gene="asnB"
                     /locus_tag="PLY56_08410"
     CDS             409..2427
                     /gene="asnB"
                     /locus_tag="PLY56_08410"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOJ21544.1"
                     /translation="MCGICGILNKRADRPVAPDLLQRMNEVLRHRGPDDAGDWSVPRA
                     NALPGNIHPRLGAPPHVSLAMCRLAIIDLVTGHQPMANEDGTLHLVLNGEIYNHCALR
                     QELQARGHHFATASDVEPVLHAYEEYGVDCVEHLEGMFGFALWDERHERLLLARDPFG
                     IKPLYYTDTGDRLLWASEIKALLQDPGVRRRVDPEALDHYLTFLYVPSPRTMFEGIHR
                     LPPGHRLIWERGTLTVEEYWRGPSAYIDPSDGARLSSAEPGGYEKALLQVLGDAIEAQ
                     LASEVPLGAFLSGGIDSTAVVLLMAERIGKPVQTFSIGFSNAGMYDELSWARIVAECC
                     RTDHREFVVGPETVELLPRIAWHLDEPLADASAIPNYLVSQLARKHVTVALTGVGGDE
                     LFGGYRRYYADAIARRWQSGILRPLARRALIEPAVRLLPPGGHTRLHNGVRLARKFFD
                     HLDRDPESRYLAWNASFTPGMKEALYVPGFRREEWTPSFSLTRPYFERVSHLPFAEQA
                     MYVDMKTYLPEDPLMMADKMTMANSLEARVPFLDRRVTEFAAALPLSQKMHGRRTKVL
                     LRNALRGRVPDDILFRPKQGFGTPIDLWLRRELRAQVARVLAPEVVAARGYFRPESVT
                     ALLQRHQQGRDDVSQHIWALFLLELWHRIFIDQDLSRRPNLGFQDLGL"
     gene            2453..3811
                     /locus_tag="PLY56_08415"
     CDS             2453..3811
                     /locus_tag="PLY56_08415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ21545.1"
                     /translation="MQLPPAQRSRPLTILMAADVDPIAVIGGAERVLNEHSKRLAARG
                     HRVIVLTRREDPARPTHEIFSGVEVYRHPVCQGNPVTFMRSTIREGARAVSQILARES
                     IDLINVHQPLAASAVLHAPGATRYPFVYTFLSPWADEFRVRAKARSTRDGRRLSNTLR
                     WAIIKAGACARRRIENHALAACRRILVLSDFSADQLWSIHGIGGERITRIPGGVDADR
                     FRPVSDRHALRRALGRPTDGLLLFTVRNLVPRMGLENLLEAMVPVVQARPDVHLVIGG
                     TGPLRDALHAQTDRLGLSEHVTFAGFIAEDLLPKYYAAADLFVLPTALLEGFGLVTIE
                     ALACGTPAVGTPIGGTQEILRDLDPRFLFRSPSADDIAAGLLERIPEVAGNQALRARC
                     RQFVEERYSWEAVIPRLEALFYATAGLPGPDPREVGAPAWASAEDGSGGGHSADGVQR
                     HS"
     gene            3924..4430
                     /locus_tag="PLY56_08420"
     CDS             3924..4430
                     /locus_tag="PLY56_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ21546.1"
                     /translation="MRIAHIITRMVLGGAQENTLLTAAAQQAAGHEVLLITGPTEGAE
                     GELVSRAEELGVRMHLVPDMVREPDPARDVRALKTLIHLLREGRYTLVHTHMSKSGVL
                     GRIAARLAGVPVIIHTPHGHVFHSYYSPAKTRLFWLLERTCALFTDRIIALTESLGDE
                     SPHPLGVG"
BASE COUNT          738 a         1509 c         1336 g          851 t
ORIGIN      
        1 tgtcggagaa ggctgaaagc gccgcaaaga cagtcgagaa ggtgaacccc gcctatacgt
       61 ctcagaactg ttcagcgtgc ggcgagagag tgctgaaggc cctctctgtc cggtggcact
      121 cctgccccta ctgcggggca gaactccacc gcgaccacaa cgccgccctg aacatccgaa
      181 agaagggcgg gggcaccgcc ttggtggatg tggcagcact ggctgccgcg tgagaaacca
      241 gaaccccaca ggctttagcc gtgggagtgt cagcgagtca cttatgtctc cggtaacctg
      301 atggggtctg tgcgatgact gaggttcctt cgcccggtat cggcccctcg gtcctgttca
      361 ctgcccggca gcggtagcgt gccggcgtct gcgagctgta ctccaactat gtgtggcatc
      421 tgtggcatac tgaataagcg agccgaccgt ccggtggccc cggacctgtt gcagcgcatg
      481 aatgaggtgc tacgtcatcg gggccccgat gacgcgggcg attggtcggt gcctcgcgcc
      541 aatgcgttgc caggcaacat acatccacgg ctaggtgctc caccacacgt gtctcttgcc
      601 atgtgccgcc tggccatcat cgatctcgtc actggtcacc aaccgatggc caatgaggac
      661 ggcaccctcc atctcgtcct caacggtgag atttacaacc actgtgcctt gcggcaggag
      721 ctgcaggcac gcgggcatca ctttgccaca gccagcgatg tcgagcctgt tctccatgcc
      781 tacgaagagt acggcgtgga ttgtgtcgag cacctcgaag gcatgttcgg ctttgccctt
      841 tgggacgagc gtcacgagcg cctgctcctt gctcgcgatc cgtttgggat caagccgctc
      901 tactataccg acaccgggga ccggctcctc tgggcctcgg agatcaaagc cctcctgcag
      961 gatccgggag ttcgccgccg cgtggatccg gaggcgctcg accactacct cacctttctc
     1021 tacgtgcctt ctcctcgcac catgttcgag ggtatccacc ggcttccacc tgggcatcgt
     1081 ctcatctggg agcggggcac gctcacggtg gaggaatact ggcgaggccc atccgcttac
     1141 attgacccca gcgatggtgc ccggctctcc agcgcagagc caggtggcta tgagaaggcg
     1201 ctcctgcagg tgctgggcga tgccatcgaa gcacaattag ccagtgaggt gcccctgggg
     1261 gcgtttctta gcggtggcat cgactccacg gccgtcgtcc tccttatggc cgagcgcatc
     1321 ggcaagccgg tgcagacttt ctccattggc ttctccaatg cgggcatgta tgacgagctc
     1381 tcctgggcac gtatcgttgc cgaatgctgc cgcacggacc atcgtgagtt cgtggtcggt
     1441 ccggagaccg tggagcttct tccccggata gcctggcacc tcgacgagcc actggctgat
     1501 gcctcggcga tcccaaacta cctcgtctcc cagcttgccc gcaaacacgt cacggttgcc
     1561 ctcaccggcg tggggggtga tgagctcttc ggtggctacc ggcgctacta tgccgatgcc
     1621 atagccaggc gttggcaaag cggtatcctt agaccgcttg ctcgccgtgc cctcattgag
     1681 cccgcagtgc gcttgctgcc gccggggggc cacactcgac tgcataacgg tgtgcgcctg
     1741 gcacgcaagt tcttcgacca tctggatcgg gacccagagt cccgctacct cgcctggaat
     1801 gcttccttca cgcccgggat gaaagaagca ctctatgtgc ctggcttccg tcgtgaggag
     1861 tggacgccct cctttagcct cacgcgcccc tatttcgagc gtgttagtca tctgccgttc
     1921 gctgaacagg cgatgtacgt cgacatgaag acctacctcc ctgaggaccc cttgatgatg
     1981 gccgacaaga tgaccatggc caactccctg gaagcccggg tgcctttcct tgaccggcgc
     2041 gtcaccgagt ttgccgccgc gctaccactc tcgcagaaga tgcacggccg ccgcaccaaa
     2101 gtgctcttgc gcaatgcgct ccgtggccga gtgccggatg acatcctctt ccggccaaag
     2161 cagggcttcg gcacgccgat cgatctgtgg ctacgccgtg agttgcgggc acaggttgcc
     2221 cgagtgctgg cacccgaggt ggttgccgcc cgcggctact tccgaccgga gtctgtcacg
     2281 gcgctccttc agcgtcacca gcagggccgc gacgatgtca gccagcacat ttgggccctc
     2341 tttctgctgg agttatggca ccgcatcttc atcgatcagg acctctcccg tcgccccaac
     2401 cttggtttcc aggatctcgg tctctagcca gggaatacac aggtaagcgc ctatgcaact
     2461 acccccggca caacgcagcc gcccgctcac catcctcatg gcagccgacg tcgatcccat
     2521 cgccgttatc ggaggggcgg aacgcgtcct caacgagcac agcaagcggt tggctgcacg
     2581 agggcaccga gtcatcgtcc tcacccggcg tgaggatcca gcccggccca ctcatgagat
     2641 cttttctggc gtagaggtat accggcatcc ggtctgccaa gggaacccgg tcaccttcat
     2701 gcgctccacc atccgcgagg gcgcccgtgc cgttagccag atcctggccc gcgagtcgat
     2761 cgacttgatc aacgttcacc aacccctcgc tgcctcggca gtgttgcacg ccccgggtgc
     2821 tacccgctat ccgtttgtct acaccttcct ctccccctgg gcggatgagt tccgggttcg
     2881 ggcgaaggca cggtcaactc gggatggccg tcgtctgtcg aacaccctgc gatgggcgat
     2941 catcaaggct ggtgcctgtg cgcgtcgacg gatcgagaac catgccctcg cggcttgtag
     3001 gcgcatcctc gtcctcagcg acttctcggc tgatcagctc tggtccatcc acgggatcgg
     3061 tggcgagcgc atcacgcgca ttccgggggg cgtcgacgcc gaccggtttc gcccggtgtc
     3121 cgaccgccac gccctgcggc gtgccctcgg aagacccacc gatggcttac tcctgttcac
     3181 cgtccgtaac ctggtgcccc ggatgggctt ggagaacctg ctcgaagcga tggtaccagt
     3241 ggtgcaggcc cgccctgatg tacacctcgt catcggcgga accggtcccc tgcgcgacgc
     3301 cctgcatgca cagaccgatc gcctcgggct ctccgagcac gtcacctttg ctggcttcat
     3361 cgccgaggac ctgctgccga agtactacgc ggcggccgat ctcttcgtct tgcctacggc
     3421 tctcctcgaa ggctttggcc tggttacgat cgaggcgctg gcttgcggca cccccgcggt
     3481 aggcactccc atcggcggta cgcaggagat cctgcgcgac ctggatccgc gctttctgtt
     3541 ccgcagtcca tcagcggacg acatcgcggc gggcctcctg gagcgcatcc ctgaggtggc
     3601 cggcaaccag gcgctccgcg ctcgctgccg ccagttcgtc gaagagcgct atagctggga
     3661 agccgtgatc cctcgcctgg aggcgctctt ctacgccacg gccggcttgc cagggcccga
     3721 tccacgcgag gtaggcgcgc cggcctgggc ctcggccgag gacggcagcg ggggagggca
     3781 tagcgccgat ggagtgcaga ggcactcgtg accgcggccc ctcaagtgac accggggccc
     3841 cgcggattcc cgcgagggcg ctccatcagc ctgccgccct gcctgccgcg gcggtatgaa
     3901 ccagtgatcg gcaatccggc ctgatgagga tcgcgcacat catcactcgc atggtcttgg
     3961 ggggagcgca ggagaacacg ctcctgaccg ctgccgccca gcaggccgcc ggccatgagg
     4021 tgcttctcat caccggacct accgagggcg ctgagggcga gctcgtctcc cgggccgagg
     4081 aactcggcgt tcgcatgcac ctggtgccgg atatggtgcg cgaaccggac ccggcccgcg
     4141 atgtccgcgc cctgaagaca ctcatccacc tcctacggga ggggcgctac acgctggtgc
     4201 atacgcacat gtctaagtcg ggcgtgctag gccgcattgc cgcgcgcctg gctggcgtgc
     4261 cggtgattat ccacacgccc cacgggcacg tcttccatag ctactacagc ccggccaaga
     4321 cccgcctctt ctggctcctg gagcgcacct gcgccctctt caccgaccgc atcattgccc
     4381 ttacagaatc cttgggcgat gaaagcccac accctttagg ggtgggatga aatc
//
LOCUS       DAOSCV010000297         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12403_length_4432_cov_3.294722, whole genome shotgun sequence.
ACCESSION   DAOSCV010000297 DAOSCV010000000
VERSION     DAOSCV010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12403_length_4432_cov_3.294722"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLY56_08425"
     CDS             <1..364
                     /locus_tag="PLY56_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21547.1"
                     /translation="YWEKKGSKESVGIVDECLGILKAINPRLELKYNRYYIGLTEDGR
                     PNNFVVFRAKKQFARAEVLLGDQASWGQRLEAAGLAVLTGEKERARIHFRISRDDPAK
                     NRELLHEIFEAAYQEQQG"
     gene            complement(610..900)
                     /locus_tag="PLY56_08430"
     CDS             complement(610..900)
                     /locus_tag="PLY56_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21548.1"
                     /translation="MPLAKIEYPADTLIIAEADWTRSTDDYGYSNSYFIQIPFHVSRF
                     IPQRHTEGANIVFVDGHAKWHKIPLDPSYTGSGSVKLTLPPAGVKWYADGSK"
     gene            complement(1027..1278)
                     /locus_tag="PLY56_08435"
                     /pseudo
     CDS             complement(1027..1278)
                     /locus_tag="PLY56_08435"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
     gene            complement(1503..>4432)
                     /locus_tag="PLY56_08440"
     CDS             complement(1503..>4432)
                     /locus_tag="PLY56_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ21549.1"
                     /translation="KTRPRIWVNAESLPQVRANLTRGENAPHWKKVQEIAAEPFEFQV
                     APNTEVSHNAKLEQAATYKAFVYLMAGDKARGREAVTLIRDYLSAVQFDNLLDITREI
                     GRAIYSAALVYDWCYDLMTPEERESIRKDLMRLADDMEIGWPPFRQTIVNGHGNEAQV
                     NRDLLCMAIALYDEDPVPYRYCAYRILEELVPMRRFEYQSPRHNQGISYGPYRFGWDL
                     HAAWLFRRMTGKPVFDDNIGEVYKFWLYMRLPIGQMLRDGDGFSDGQQVNLGLTPLLA
                     YAYTRDPVIKGDFVRQGFRADPLMILLLNDPELPAQKSLDHLPLTIDFGPVLSGMVAR
                     TGWNLGRNLADVVVEMKGGGYHFGNHQHSDAGSFQIYYRGLQAVDLGQYRFYGTPYDT
                     NFCKRSVAHSMMLVVDPEEKFGRAPANDGGSRFVQTCPTTVEQVLNNPMFAYGKKVSS
                     SFGPSPQRPFYSYFSVDLKSAYSEKIQEFVRTFCFLNLGNLETPAALIILDHITAAKP
                     EFRKYWQVNTLNPPQPTPDGVILRNSALGLSGKVSVRMLRPRPEEREMQVLSGEATHN
                     VFGQQFTPPSPEKPEGRGHRVLFSPKTPRASDVFLTVLPMSDEKAPELPVSLSETETT
                     FALTLADRVVVLSKSSQFLKEPFQVQTPAGEESQLLLTGLAPGAWSIRTRDNRIRFNA
                     RVEAGKNTAFFVVPGGDYEVRPEAIPGAPEYQAPADLVPALAPSLNHRVLLNDQVLPT
                     PPVRSADTHLLVPALAVFKALGIETEEAGGALRVRARGRSARFVAGSTTLELNGRRFQ
                     MPGPAVQEKGEWYLPDSVVAAVAGQELTRDQEGVGVTLAPSRIPGDDAILWIEANKES
                     DLAALRAMLADIPGRREYWAAEGKDVHFDLVLARPVKLRGVGIRWHQGAARQARFALE
                     TSSDGVTWRKVFDGASSGKSADLETYTFDPHEAGYVRFRGFGNTQNGWNSIVHFRLLP
                     AEP"
BASE COUNT          794 a         1426 c         1385 g          827 t
ORIGIN      
        1 ctactgggag aagaaaggct cgaaggagtc ggttggcatc gtggacgagt gccttggcat
       61 cctgaaagcg atcaacccga ggctcgaact gaagtacaac cggtactaca tcggtctcac
      121 cgaggatgga cggccaaaca acttcgtcgt gttccgcgcc aagaagcagt ttgcccgggc
      181 agaggtgtta ctcggtgatc aagcgtcctg gggccagcgc ctcgaagcgg ccggtctggc
      241 agtcctcact ggcgagaagg agcgagcgcg catccatttc cgtatctcaa gggacgaccc
      301 ggcgaagaac cgtgagctgc ttcacgagat attcgaggca gcctatcaag aacagcaggg
      361 ctaaggcacg ccatcacccc gcatcctctg ttcacaccac ccgtgtctgg ctcggcgggc
      421 aatgcctgta cgcaggctgt cattggatac atagcccgcg ttggccttga gcacttcggt
      481 gatttcgccg tgtccgccca ccattcaaag cggctccatc ttcgcttagt cgagcacctt
      541 tccaacaata aggggcgcgg gccccacttc tggacccgcg cccacttcca gctcttcgcg
      601 atctgcccgc tatttggagc cgtcggcgta ccacttcacg ccggccggag ggagcgtcag
      661 cttcaccgag cccgagccgg tgtagctcgg atcgagcgga atcttatgcc acttggcatg
      721 gccgtccacg aagacgatgt tcgcgccttc cgtatgtcgc tgcgggataa agcgcgagac
      781 gtggaacgga atttggatga aataggagtt gctgtagccg taatcgtccg tgctgcgcgt
      841 ccaatccgcc tctgcgatga tgagcgtatc ggcaggatac tcgatcttcg ccagcggcat
      901 atcgccggcc gcaagcgaga ggtgcgtgtt gatgccgtac gcggtatcgc ccgagtagtt
      961 ccccttccac accttcccgc tgctggggca gttcatcacc ccggtgctct tgtgatacgg
     1021 ctccagtgcc atataccata gcagtttcga gtggttgtag ccgttatagg tgaaaggcgc
     1081 tgggagctcg atctggcgcc gcgggagaag ttcatcgtag tcctggtaat acatcatcag
     1141 ggcgttgccc atctgcttgc agttgctcat gcaggtggtc ttgcgcgcgt tctcgcgtgc
     1201 cctggcaaag accgggaaca gaatcgccgc cagaatcgcg atgatagcaa tgactacgag
     1261 cagctcgatg agcgtgaagc ccctgtgtgt gtaggagagt catggcagtc tcctttcatg
     1321 catattcgct agtgaaagtc tagcacaggc gctctcacct gtcaacgccg cgtttcccgc
     1381 gagatcgccg aggaaactct cgcttcaaac tcctctggtt cggtgcccgg catcccccgc
     1441 ctcccacccc tggccgggcg cgtgccccgc gcctatccgg cgaagggcac gcgcctcatg
     1501 catcacggct cggctggaag caggcggaag tggacgattg agttccaacc gttctgcgtg
     1561 ttgccgaaac cacgaaaacg cacgtagccg gcctcgtgcg ggtcaaaagt gtacgtctcc
     1621 aggtcggccg acttgccgga gctggcaccg tcgaacacct tccgccacgt gacgccatcg
     1681 ctgctggtct cgagggcaaa tcgcgcctgg cgggcggcgc cctgatgcca tcggatgcca
     1741 acccctcgca gcttcaccgg ccgggcgagc accaggtcga agtgcacgtc cttcccctcg
     1801 gccgcccaat actcgcgcct gccgggaata tccgcgagca tggcgcgcaa cgccgcgagg
     1861 tctgactcct tgttcgcctc gatccagagg atcgcgtcgt caccaggaat ccggcttgga
     1921 gccagggtta cccccacgcc ttcctgatcc cgcgtgagct cctgtccggc caccgcggcc
     1981 acgacgctat cggggagata ccactccccc ttctcctgta ccgccgggcc cggcatctgg
     2041 aaccgccgcc cattgagctc gagcgtggtg gagcctgcca caaagcgcgc gctccggccc
     2101 cgggcgcgca cgcgcagggc gccgccagcc tcctcggttt cgatccccag cgccttgaaa
     2161 accgccagcg ccggcacgag caggtgcgta tccgccgagc gcacgggcgg cgtcgggagc
     2221 acctggtcgt tcaagagcac gcgatggttg agcgacggcg cgagagccgg caccaggtcc
     2281 gccggagcct ggtactccgg cgccccagga atcgcctccg ggcgcacctc gtagtcgccg
     2341 ccgggcacga cgaagaacgc ggtgtttttt cccgcctcca cacgggcgtt gaagcgaatc
     2401 cggttgtcgc gggtgcgaat gctccaggca cccggggcca ggccggtcag caggagctgt
     2461 gactcctcgc ccgcgggggt ctgcacctga aacggctcct tcaggaactg gctgctcttg
     2521 ctcaaaacga ccacccggtc tgccaaggtg agcgcgaacg tcgtctccgt ttccgacagc
     2581 gagacgggca actccggcgc cttctcgtca gacatgggga gaacggtgag gaagacgtca
     2641 ctggcacgcg gcgtcttggg cgagaagagg acgcggtggc cacggccctc aggcttttcc
     2701 ggggagggcg gggtgaactg ctggccgaag acgttatgcg tcgcttcgcc gctcaggacc
     2761 tgcatctcgc gctcctctgg gcgcgggcgc agcatccgga cgctcacctt cccggagagg
     2821 cccagggcgc tgttccgaag gatcacgcca tctggcgttg gctgcggtgg gttcagcgtg
     2881 ttcacctgcc agtacttccg gaactccggt ttcgctgccg tgatgtgatc caggatgatc
     2941 agggccgccg gcgtctccag gttgcccagg ttgaggaagc agaaggtacg cacgaactcc
     3001 tggatcttct cgctgtaggc cgatttcagg tctacggaga agtagctgta gaacggccgc
     3061 tggggcgagg gcccgaagct ggacgacacc ttcttcccgt aggcaaacat cgggttgttc
     3121 agcacctgct ccacggtcgt cgggcaggtc tggacaaatc gggagccgcc atcgttcgcc
     3181 ggggcacgcc cgaacttctc ctccgggtcc accaccagca tcatgctatg cgcgaccgag
     3241 cgcttgcaga agttggtatc gtagggcgtg ccgtagaacc ggtactggcc gagatcaacc
     3301 gcctgcaggc cccggtagta gatctggaag ctgcccgcat ccgagtgctg gtggttgccg
     3361 aagtgatacc cgcctccctt catctcgacc acgacatcgg ccaggttgcg cccgaggttc
     3421 cagccggtgc gagcgaccat gccgctcagc acggggccga agtcaatcgt cagcggcaga
     3481 tgatccaggc tcttctgcgc gggcagctct gggtcattca gcaggaggat catgagcgga
     3541 tcggcccgga agccctggcg gacgaagtca cctttgatga ctggatcgcg cgtgtaggca
     3601 taggcgagca gtggagtcag gcccaggttc acctgctggc catcggagaa gccgtcgcca
     3661 tcgcggagca tctgcccgat gggcaggcgc atgtagagcc agaacttata gacctcgccg
     3721 atgttgtcat cgaagacggg cttgccggtc atccggcgga agagccaggc cgcgtgcaag
     3781 tcccagccaa accggtaggg gccatagctg atgccctggt tgtgccgcgg cgactggtac
     3841 tcgaaccggc gcatcggcac cagctcctcc aggattcggt aggcgcagta gcggtagggc
     3901 accggatcct catcgtagag ggcgatcgcc atgcaaagga ggtcgcggtt cacctgcgct
     3961 tcgttgccat gcccgttgac aatcgtctgc cggaagggcg gccagccgat ctccatgtcg
     4021 tctgccaggc gcatcagatc cttccggatg ctttcacgct cctccggcgt catcaggtcg
     4081 tagcaccagt cgtagaccaa cgccgccgaa tagatcgccc ggccgatctc gcgcgtgata
     4141 tcgaggaggt tgtcaaactg cacggccgag aggtagtctc ggatcagcgt caccgcttcg
     4201 cgcccgcgcg ccttatcacc ggccatcaga tagacaaacg ccttgtaggt agcagcctgc
     4261 tccagcttgg cgttgtggct cacttcggta ttcggcgcca cctggaactc gaacggctcg
     4321 gccgcgatct cctgaacctt cttccagtgt ggagcgttct ctccccgggt gaggttggcg
     4381 cgcacctgcg gcagcgactc cgcgttaacc cagattcgcg ggcgcgtctt gg
//
LOCUS       DAOSCV010000298         4430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12411_length_4430_cov_3.241600, whole genome shotgun sequence.
ACCESSION   DAOSCV010000298 DAOSCV010000000
VERSION     DAOSCV010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12411_length_4430_cov_3.241600"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLY56_08445"
     CDS             complement(<1..373)
                     /locus_tag="PLY56_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ21550.1"
                     /translation="MEASVVWDHGLSFTGTAGSGFEVALGGAREAGGDEDGFRPLELM
                     LISLAGCTAMDVILIIKKQRQAVTGLEVKVHAERAAEHPKVFTAIEVEYIVRGRDIKE
                     AGLEKAIELSETRWQYDVDRLI"
     gene            complement(450..1001)
                     /locus_tag="PLY56_08450"
     CDS             complement(450..1001)
                     /locus_tag="PLY56_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21551.1"
                     /translation="MPQDLRRQALASVLPQDYRLDRLGVTIRAAEPSDLPTISCLRSD
                     EPERARRRLDSQESGHVLYLLALINNLPVGTALLHWQSITRVPVGASVSDLYVIPQLR
                     GLGIGTQLLKTSEALARAAGLHQISLAVNPTENRRVRHYYERLGYRDPGGQTYLNATY
                     TRVDENGNEYVFEDWVVDMVKRL"
     gene            1352..2137
                     /locus_tag="PLY56_08455"
     CDS             1352..2137
                     /locus_tag="PLY56_08455"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ21552.1"
                     /translation="MIRKALVIAVLLAWPALGHAQSLSITGPARSGLLEGELYRITWK
                     ATGLKGISVVATGERTPLGTNSRGTFTTIIAESAPASRGTAPWRVPWIDAVELTIKVK
                     GYDARGQLVATDMRRYHFRPAVMAHRMKNGIYLDLHLPRRQRLYVQRDGRITHAFLSS
                     SSARYSWQPRNRHPAAPHDHAGVYRVLEKRKMHWSQLFDVPMPYAIRYHGGHFIHATS
                     RNLYAHLGRPASAGCNRLTRHDASLLYQMTPIGTRVEVIGPGG"
     gene            complement(2152..2814)
                     /gene="uppS"
                     /locus_tag="PLY56_08460"
     CDS             complement(2152..2814)
                     /gene="uppS"
                     /locus_tag="PLY56_08460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOJ21553.1"
                     /translation="MGSKQFARLPRHVGFIPDGNRRWAEQRGLPRSEGYAAGIEPGLL
                     LLDECARLGIAEVSIYGFTQENTRRPKEQTQAFRAACVEFALRAAARGVALRVLGDAT
                     SPVFPPELIPYAERRVGPGPLRVNLLVNYGWAWDLENALRHGASAGRRDVMQCLASAD
                     ASRLDLVVRWGGRCRLSGFLPVQSVYADLYVVDALWPDFQIEQFHEALRWHARQDVTL
                     GG"
     gene            complement(2918..3688)
                     /locus_tag="PLY56_08465"
     CDS             complement(2918..3688)
                     /locus_tag="PLY56_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21554.1"
                     /translation="MASEDARWLLRCAAGHRDGHLWWWALAAFVLLLVLWNSWEAPQG
                     EGGLQPSDTTRGAATAWGAFTGVAATVGDVTAAPHSFLGVIVTVSGEVSRVIDARAFT
                     LGGKMFGCPELLVIAASPIPGIQERPPQTPLLAGDLVLVTGPVHSFNRVHFEREAGVV
                     LGKARFAGWSGRPAILALDVRVTPRLLPVPGTLPAAPATGAAPPIGSAPALPASPVPS
                     PPAMGYIPEPPGNAPAVPSTPAFPGMGAPPGLTGPPNG"
     gene            complement(3861..4259)
                     /locus_tag="PLY56_08470"
     CDS             complement(3861..4259)
                     /locus_tag="PLY56_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21555.1"
                     /translation="MPEFTNPFIGTVPRQMTHEELVRAIMLNISAEFEAVHLYLAHRD
                     ATTNEDARKVLYDIALEELVHVGEFTSLLYRLDPVSAQKVEEGIEEVRELLDSKAPAE
                     ASAGAEAAEGTESADASRRLTVGSLLGKEG"
BASE COUNT          799 a         1507 c         1401 g          723 t
ORIGIN      
        1 tgatcaaccg gtcgacatcg tactgccagc gggtctccga gagctcgata gccttctcca
       61 gaccagcctc cttgatatcg cgcccgcgca cgatgtactc cacctctatc gcggtgaaga
      121 ccttcgggtg ttcggcggcg cgttccgcgt gaaccttcac ctccagcccc gtcactgctt
      181 gccgttgctt cttgatgatc aggatcacat ccatcgcggt gcatccggcc agcgagatga
      241 gcatcagctc cagcgggcga aacccatcct cgtcgccccc cgcctcgcgc gctcctccca
      301 gagccacctc aaacccactg ccggccgtcc ccgtgaagga gagaccgtgg tcccacacca
      361 ccgaagcttc catccgcctg ccctccgttc gcttgctgtc ccgggggcct cgtggccatc
      421 gtggtgaggc ccctcctgcc ggtgacggtc tagagacgtt tcaccatgtc cacaacccaa
      481 tcctcaaaga cgtactcgtt gccgttctca tcgacgcggg tgtacgtggc gttgaggtag
      541 gtctgccctc caggatcgcg atagccgagg cgctcgtagt agtgacgaac ccgacggttc
      601 tcggtcgggt tcacggcgag tgagatctga tgcaggccag cggcccgggc gagtgcctca
      661 ctcgttttga gcaactgcgt gccgatgccg aggccgcgaa gctgcgggat cacgtagaga
      721 tcggacacgc tggcgcccac cggcacacgc gtgatgctct gccagtgcag gagcgcagtg
      781 ccgaccggca gattgttgat gagcgcgagg agataaagaa cgtgccccga ctcctgagag
      841 tccaggcgcc ggcgcgcacg ctctggctca tccgaacgga ggcaggagat ggtcggtaga
      901 tccgaaggct cagcagcccg gatggtgacg ccgaggcgat cgagacggta atcctgcggg
      961 agcaccgagg caagggcttg cctgcgcagg tcttgcggca tggttcccct cctttgcttc
     1021 gacgaacatg gccaaaccgc cggaggcgag cctcccggct gcgcgccgaa ctcgggaagc
     1081 cagcgcgggc tccgaaggat ggctcgcgaa aaccgttgcc gatggcggcc gccagacgag
     1141 tccagcttcc cgatcctaca ggcagtccgc ggcgtcgaca gcacctcggg ccggtgccgg
     1201 gcttagtcgc cgcgactcgt caggcgttgc cacgggggcc tctccactcc ccctcggtat
     1261 tatccttttg ttcgggcatc gcgcaggaac tcctcccatc cccctcgttt cggggtcccc
     1321 ggggcgaggt ataccagtcc gagggaacat catgatacga aaagcgctgg ttatcgcggt
     1381 actcctggcg tggccggcgc tcggccatgc ccaatcactc tcaatcaccg ggcccgctcg
     1441 ctccgggctc ctggagggcg agctctaccg gatcacctgg aaagcgacgg ggctgaaggg
     1501 catcagcgtg gtggccaccg gcgagcggac gcccctgggc acgaactcgc gtggcacatt
     1561 caccacgatc atcgcggaga gcgcgcccgc atctcgcggc acggcgccgt ggcgcgtgcc
     1621 atggatcgat gccgtggagc tgacgatcaa ggtgaagggt tacgacgcgc gcgggcagct
     1681 cgtcgccacc gatatgcgcc gctaccattt ccgtccggcg gtgatggcgc accggatgaa
     1741 gaacggcatc tacctggact tgcacctgcc gcggcggcag cgactgtacg tgcaacgcga
     1801 cgggcggatc acccacgcct tcctctccag ctcctcggcg aggtactcgt ggcaaccgcg
     1861 gaaccggcat ccggcggcgc ctcacgacca cgccggcgtc taccgcgtgc ttgagaagag
     1921 gaagatgcac tggtcgcagc tcttcgatgt gccaatgccc tacgcgatcc gctatcacgg
     1981 cgggcacttc atccatgcga ctagccgcaa tttgtatgcg cacctgggcc ggcccgcatc
     2041 cgccggatgc aaccgcttga cgcggcatga tgcgagcctg ctgtatcaga tgacgccgat
     2101 tggcacgcgc gtcgaagtga tcggccctgg cgggtgaggc tggcgcgtgt ctcacccgcc
     2161 gagggtgaca tcctggcgag cgtgccagcg cagcgcctca tgaaactgct cgatctggaa
     2221 atcgggccat agggcatcga ccacataaag atcggcgtag accgactgca ctgggaggaa
     2281 accgctcaag cggcatcgcc ctccccagcg cacaaccaga tcgaggcgcg aggcatccgc
     2341 cgatgccagg cactgcatca cgtcccttcg gcccgcgctg gcgccatggc gcaaggcgtt
     2401 ctccagatcc caggcccagc cgtaattcac caggaggttg acccgcaggg gtcctggccc
     2461 cactcgccgc tcggcgtaag gaatcagctc tggaggaaag acaggagatg tggcatctcc
     2521 cagaacgcgc agggcgacgc cacgggccgc ggcacgcagg gcaaactcga cacacgcagc
     2581 ccgaaacgcc tgggtctgct ccttcgggcg gcgcgtattc tcctgggtga atccgtagat
     2641 cgagacttcc gcgatgccga ggcgcgcaca ctcgtcaaga aggagcaaac ccggctcaat
     2701 tccggcagca tacccctcgc tccggggcag gccgcgttgt tctgcccaac gccgattgcc
     2761 atccggtatg aagccgacgt gcctgggtag gcgcgcgaac tgttttgaac ccatgccctc
     2821 ctcccacgcc gcggatccgg ggagaaggcg ggggatccca ttccgggatg gagtgaacgg
     2881 ctcccaccgc atccgagcgg catgatgcgg agcagagtta cccatttggc gggccggtca
     2941 aacccggtgg ggcacccatg ccgggaaaag caggcgtcga gggcacagca ggcgcgttcc
     3001 caggaggctc cggaatatag cccatcgccg gaggggacgg caccggactc gcgggcaggg
     3061 cgggcgcact ccctatggga ggcgctgcgc ccgtcgccgg cgcagcgggc agcgtgcccg
     3121 gcactggcaa gaggcgtggc gtgacccgca catcgagagc gaggatcgct ggccggccgc
     3181 tccatcccgc gaaccgagcc ttccccagga cgactcccgc ctcgcgttcg aaatgaaccc
     3241 ggttgaagct gtgaactggg ccggtgacga gcaccagatc gcccgccaag aggggcgtct
     3301 ggggaggccg ctcctgaatg ccaggaattg ggctggcggc gatgacgagt aactcggggc
     3361 atccgaacat cttgccgccg agggtgaacg cgcgggcgtc aatgacgcgg ctcacctcgc
     3421 cgctcacggt cacaattacg ccaaggaagg agtgcggcgc cgcggtaacg tcgcccacgg
     3481 ttgcagcgac gccggtgaag gccccccatg ccgtcgctgc gccgcgcgtc gtatctgaag
     3541 gctgaaggcc cccttcgccc tgtggagcct cccagctatt ccataggacc agcaggagca
     3601 cgaaggcggc gagggcccac caccagaggt ggccgtcacg atgtcccgca gcgcagcgca
     3661 gcaaccatcg ggcatcctcg cttgccaccc tctcccccaa ccacccatgc gctgagcaag
     3721 gcagactgcc gacgcaaccg gcgtcgcctc atgccatggc gtcactccgc ggccggtcat
     3781 ccagcgttag acccggccgc gagatccacg ccaacggtat accctcgccg cccgtgcatc
     3841 aaaacggagc gcgaccgaac ttagccctct ttccctagca agctgccaac ggtgaggcga
     3901 cggctcgcat cggcgctttc cgtgccctcc gcggcctctg cgccagcgga tgcctcggca
     3961 ggcgctttgc tatcgaggag ctcgcggact tcctcgatcc cttcctcaac tttctgcgcg
     4021 gagaccggat ccaatcggta gaggagactg gtgaactcgc ctacatggac caattcctcg
     4081 agagcaatgt catacaggac cttgcgcgca tcctcgttgg tcgtggcgtc tcggtgggcc
     4141 aggtagagat ggaccgcctc aaattccgcc gagatgttga gcatgatggc acgcaccaac
     4201 tcttcgtgcg tcatctggcg gggaaccgtt ccaatgaaag ggttggtaaa ctcgggcatg
     4261 cggtgactcc tcccccggcg tcgctctccc atgcgttttc tcgacagtgg tgatatgggc
     4321 aggcgcccgc gtcactgacg acaggagcga cgcgcgcttg gatgcccgca ccgatgcgct
     4381 tcccctgcac ggcagaggta caggcgcatg gaggacaccc cttaaggcat
//
LOCUS       DAOSCV010000299         4401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12518_length_4401_cov_4.486884, whole genome shotgun sequence.
ACCESSION   DAOSCV010000299 DAOSCV010000000
VERSION     DAOSCV010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_4401_cov_4.486884"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /gene="arsB"
                     /locus_tag="PLY56_08475"
     CDS             complement(<1..984)
                     /gene="arsB"
                     /locus_tag="PLY56_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOJ21556.1"
                     /translation="MLKPPVSQPVDAGCPSSISGQLSFLDRYLTLWIFLAMAIGVGSG
                     FAIPGLVKAITALQVGTTSIPIAVGLILMMYPPLAKVRYEELGEVFRNTRVLGLSLVQ
                     NWIVGPILMFLLAITFLRGYPEYMVGLIMIGLARCIAMVIVWNELAKGDTEYAAGLVA
                     FNSVFQVLFYSVYAYLFITVLPPLFGLHGAIVRVSIGQIAESVAIYLGIPFFSGMITR
                     FVLLKAKGREWYEKEFIPRISPITLVALLFTIVVMFSLKGEVIVRLPLDVLRIAVPLL
                     IYFVVMFLVSFAMGRRLGADYSKTATLSFTAASNNFELAIAVAVAVYGINSG"
     gene            complement(984..1394)
                     /locus_tag="PLY56_08480"
     CDS             complement(984..1394)
                     /locus_tag="PLY56_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ21557.1"
                     /translation="MAGEVAQYPRTRLTRGIDAQDALRRADEVESLAQRLSALSDGNR
                     LKILHLLHTCGEMCVCELQDVLGLTAPNLSFHLKILRYTGFVRARKQGKWVFYRAVSE
                     QARGTGDAVRALFQGDPPQVSDSACAPCGTGEEE"
     gene            complement(1783..3462)
                     /locus_tag="PLY56_08485"
     CDS             complement(1783..3462)
                     /locus_tag="PLY56_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOJ21558.1"
                     /translation="MSWLLELIWGESIAHAILILALVAGTGLALGHLRVAGIGLGIAG
                     VLFTGLLFGHFGLGINAEVLDFAREFGLILFVYSIGIQVGPGFFDSLRRQGLPLNLMA
                     AGIILGGVVVTLLIHFLAGIETPIAVGLFSGATTNTPSLAAAQQALRDVPGFTTEMAK
                     LPGLGYAVAYPFGVIGIILTMLLVRSAFRIDLMRETEALKEEQAAGGRASLARMHLEV
                     KNPGVDGQRVREVLAQLGSEAGVVISRILHGGEIQVALPDTRIHQGDVILAVGPRDRL
                     EALRDLVGTESPIDLTSIESEITATRIVVTRKAIVGKSVAELNLPERHGVTITRVTRA
                     GVEFSPGPAVRLQLGDSLLAVGPRDAIERAGRELGNSARELGVPEIIPIFTGIALGIL
                     LGSWPIPVPGVPAPVKLGLAGGPLVVAILLSRVGHIGPLFWYLPQSANLVLREIGIVL
                     FLAAVGLRAGDRFVETLVSGDGFVWMGLAALITLVPLLVVALIGRLFLKVNYLTLCGL
                     LAGSMTDPPALAFAGSFTRSEAPSVAYATVYPLTMILRVLSAQLMVLLLMR"
     gene            complement(3492..>4401)
                     /locus_tag="PLY56_08490"
     CDS             complement(3492..>4401)
                     /locus_tag="PLY56_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21559.1"
                     /translation="AGWVRADGTATPALNVVAEMAAFLERNQQRMGDPLPSPVAMVVP
                     SSRLLSAPDAAALAIRRCIRTLVYRNAMPLRAIGEYSLQELESARLMLLPSPRVLQQE
                     AWETLLSRVEEGACLLVTGPFDRDRYWQRHDRLSAFELHARTQPVAREEVLSLYGDPV
                     RLHFGETKTEWVEKAVIEGYPPQVMALKHGRGQLLFSPLPLELADDVEPLAELYETAI
                     VESGVERPFDMEPRPSGVLAIPLCFESVVLYALVSQTDTACRVTLRHGPDRVAIPVDV
                     PAQRAVLLLVERGTGKVLDHYRPAGV"
BASE COUNT          911 a         1460 c         1365 g          665 t
ORIGIN      
        1 gccggaattg atcccgtaca ctgccacggc caccgcaatc gccaactcga agttattgct
       61 tgccgccgtg aaagagagag tggccgtctt ggaataatcg gccccaagtc tccggcccat
      121 cgcgaacgac accaggaaca tcaccacgaa gtaaatcagc agtggcacgg cgatccgcag
      181 cacgtcgagc ggcaggcgga caatcacctc tcccttcaga gagaacatca ccacgatagt
      241 gaagagcagg gccaccaggg taatcgggct gatccggggg atgaactcct tctcatacca
      301 ctcccggccc tttgccttca ggagcacgaa gcgggtgatc atgccgctga agaacgggat
      361 gcccagataa atcgccacgc tctccgcaat ctgcccgatg gatacccgga caattgcccc
      421 gtgcaggccg aacagcggcg gcaggacggt gataaacagg taggcataga cggaatagaa
      481 caatacctgg aaaacgctgt tgaaggcgac caacccggcg gcgtattccg tatccccctt
      541 cgccagctcg ttccagacga tcaccatcgc aatacaccgg gccagcccaa tcatgatcag
      601 gcccaccata tactccgggt agccccgcag gaaggtaatc gccagcagga acatcaggat
      661 ggggccgaca atccagttct gcaccagtga taatccaagg acgcgggtgt tgcggaagac
      721 ctctcctaac tcctcgtatc tcaccttcgc gagcggcggg tacatcatca ggatgaggcc
      781 cacagcaatc gggatagagg tggtgcccac ctggagcgcc gtgatcgcct tgaccaaccc
      841 gggaatggcg aacccggagc cgaccccaat cgccatcgcc aggaatatcc acaacgtcag
      901 gtagcggtcc aggaaagaga gttggcccga gatactggaa gggcacccgg catccacagg
      961 ttgagagact ggcggtttca acattattcc tcctcccccg tgccacaggg ggcgcaggct
     1021 gagtcagaca cctgcggcgg gtcgccctgg aagagcgccc gcaccgcatc tccggtgcct
     1081 ctggcctgct cagacaccgc ccggtagaag acccacttac cctgcttccg ggctcttacg
     1141 aacccggtat agcgcagtat cttcaggtgg aaagagaggt tgggggccgt tagccccaac
     1201 acatcctgca gttcgcacac gcacatctcc ccgcaggtgt gcagcaggtg gagtatcttc
     1261 agacggttcc catccgacag cgcactcagg cgctgcgcga gtgactccac ctcatccgcc
     1321 cgcctcaagg cgtcctgagc gtctatcccg cgggtgagcc gcgtccgggg atactgggct
     1381 acctctcctg ccatgccgct tcctctccgt caccagagct ctataccggc atttgattcg
     1441 aagctcgggt tgcttcaaca ttcaagctgg tatttaattg agactagtat agcacttccg
     1501 ccgaacacga tcaaggcggc ggaagggtgc ctgggattcc gagataagcg cacgcgcgga
     1561 gctcgaagct ccgcgctgct tgtgagccaa gccctgccgg gctgggttgg ggatactccc
     1621 agacgttgca aaacggcggt ggctgggagg ggaagccctg aaagggcttt gccggaaagc
     1681 agcgcggtgc ttttagcgcc gcgcgccggg ttgcgcgctg catccgaggc ttcggattcc
     1741 ttatctcgga atctcagaaa ggtgccaagc cgggcacttg cctcaccgca tgaggaggag
     1801 caccatgagc tgggcactca gcacccgcag gatcatcgtc agtgggtaga ccgtcgcgta
     1861 ggcgacagaa ggcgcctccg accgggtgaa actgcccgcg aacgccagcg ccggcgggtc
     1921 ggtcatgctg cccgccagga ggccgcacag cgtcaggtag ttcaccttca ggaagagccg
     1981 gccaatgagg gcaacgacga gcagcggcac gagagtgatg agcgccgcca gccccatcca
     2041 gacgaagcca tccccagaga cgagggtttc gacgaagcgg tcgccggcgc gcaagcctac
     2101 ggcggcgagg aagaggacaa tcccgatctc gcggaggacc aggttggcgc tctggggcag
     2161 gtaccagaag agcgggccga tatgtccaac gcggctgagg aggatggcga cgacgagagg
     2221 cccgcccgcc agcccgagtt tcaccggcgc gggcacgccc ggcaccggga tgggccagct
     2281 tccgagcagg atgcccaggg caatcccggt gaagatcggg atgatctccg gcacccctag
     2341 ctcccgggcc gagttgccca attccctgcc agcgcgctca atcgcgtcgc gcggcccaac
     2401 cgccaggagg gagtcgccca actggaggcg gactgccggt ccaggcgaga actccacgcc
     2461 ggcgcgcgtg acgcgggtga tcgtgacgcc atgccgctcc gggagattca gctccgccac
     2521 cgatttgcct acgatcgcct ttcgcgtgac gacgatgcgc gtcgccgtga tctcgctctc
     2581 gatgctggtc aggtcgattg gggattcggt gcccaccagg tcgcgcaacg cttcaagccg
     2641 gtcgcggggg ccaaccgcca gaatcacatc cccctggtgg atgcgcgtgt cgggcagggc
     2701 cacctggatc tcaccgccgt ggagaatacg cgagatgacg accccggcct cggatccgag
     2761 ctgcgcaagc acttcacgca cgcgctgtcc atccacgccg gggttcttca cctccaggtg
     2821 catccgcgcg agtgaagcgc ggcctcccgc agcctgctcc tccttcaggg cttccgtctc
     2881 ccgcatcagg tcgatccgga aggcggaccg gacgagcagc atcgtgagga taatgccgat
     2941 caccccgaag ggataggcga ccgcgtagcc cagaccaggg agcttcgcca tctcggtcgt
     3001 gaagcccgga acgtcgcgca gggcctgctg ggccgccgcg agactgggcg tgttggtcgt
     3061 ggcgccggaa aagaggccca ccgcgatggg ggtctcgatt ccagccaaga aatgaatcag
     3121 gagggtgaca accacccctc cgaggatgat gccggcggcc atcagattga gcggcagccc
     3181 ctgtcggcgg agcgagtcaa aaaagccggg gcccacctgg atcccgatgg agtagacaaa
     3241 gagaatcagc ccgaactcgc gggcgaaatc gagtacctcc gcgttgatcc cgagcccgaa
     3301 gtggccaaac aggagcccgg tgaacaggac gccagcaatg cccaaaccga tgccggccac
     3361 gcgcaggtga cccagcgcaa gccccgtccc cgccaccagg gccaggatca ggatcgcgtg
     3421 cgcgatcgac tctccccaaa tcagctccaa caaccaagac atccgatctc ttctctcttc
     3481 ccgtacaccc ctcagacccc agccggccga tagtgatcga gcaccttccc cgtgccgcgc
     3541 tccacaagca gcagcaccgc gcgctgggcc ggcacatcca ccgggatcgc gacgcgatcc
     3601 ggaccatggc gcaaagtgac ccggcatgct gtatcggtct gcgaaacgag cgcgtagagg
     3661 acgacgctct cgaaacaaag cgggatcgcg agcacgccac tgggtcgtgg ctccatgtcg
     3721 aacgggcgct cgacgccgct ctccacgatg gcggtctcat acagctccgc gagcggctcc
     3781 acatcgtcgg ccagctcaag cggcaaaggc gagaagagaa gctggccccg gccatgcttc
     3841 agcgccatca cctggggcgg atacccctcg atcaccgcct tttccaccca ctccgtcttc
     3901 gtctcgccaa agtggagacg caccgggtcg ccatacagcg agaggacctc ctcacgggcg
     3961 accggctgcg ttcgcgcgtg cagctcgaac gccgacagcc gatcgtgcct ctgccagtag
     4021 cggtcgcggt caaacggccc ggtaacaagc aggcaagcac cctcctcgac acgggaaagg
     4081 agggtctccc aggcttcctg ttgcagcacc cgcggggagg gaagcagcat gagccgggca
     4141 ctctccaact cttgcaggga gtactcgcca atggcccgga gaggcatagc gttgcggtaa
     4201 accagcgtgc ggatgcaccg cctgatggcg agagcggccg cgtcgggcgc cgagaggagc
     4261 cgcgaggaag gcacgaccat cgccacgggg gaggggagtg ggtcgcccat ccgctgctgg
     4321 ttccgctcca ggaaggcagc catctccgcc accacattga gcgccggggt cgcggttcca
     4381 tcggcgcgca cccacccggc g
//
LOCUS       DAOSCV010000300         4383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12574_length_4383_cov_3.315388, whole genome shotgun sequence.
ACCESSION   DAOSCV010000300 DAOSCV010000000
VERSION     DAOSCV010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12574_length_4383_cov_3.315388"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="atpD"
                     /locus_tag="PLY56_08495"
     CDS             complement(<1..846)
                     /gene="atpD"
                     /locus_tag="PLY56_08495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ21560.1"
                     /translation="MAEVNQGGATGHVVQIIGPVLDIRFPAGQLPKILNAIEVEMPQN
                     GQTSRIIAEVAQDLGNDTVRCVAMAPTDGLPRGAKARDTGQPITVPVGRNTLGRVFNL
                     LGEPVDDMGPVKGNDYYPIHRPAPSFEEQSTEMQILETGIKVIDLLCPYLKGGKIGLF
                     GGAGVGKTVLVQELIRNIATEHGGFSVFAGVGERTREGNDLWLEMRESGVIEKTTMVF
                     GQMNEPPGARLRVALSALTMAEYFRDVERQDVLLFIDNIFRFTQAGSEVSALLGRIPS
                     AVGYQP"
     gene            complement(855..1721)
                     /gene="atpG"
                     /locus_tag="PLY56_08500"
     CDS             complement(855..1721)
                     /gene="atpG"
                     /locus_tag="PLY56_08500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOJ21561.1"
                     /translation="MASMRDIRQRIRVVRNIQKITSAMQMVAAARLKRAQNRVGAARP
                     YAEKMAEVMRHLAGAASGIEHPLLEVREPRRMAVVVITADRGLCGSYNTNIVRTAMEL
                     LSEHKDLESVVLPVGRKGPSAFRQRGCTLGATFELPSRDVALADAQAITREVRRLFGS
                     GEIDIAYLVYTRFISALRLQPTVQQLLPLQPPQAAESRLQGVEEYLFEPVPERFLASL
                     LPRYLDVQVYQALVESVASEHGARMTAMRSATDNAAEMIDRLTLSLNRARQAAITREI
                     AEIVGGADALKG"
     gene            complement(1856..3364)
                     /gene="atpA"
                     /locus_tag="PLY56_08505"
     CDS             complement(1856..3364)
                     /gene="atpA"
                     /locus_tag="PLY56_08505"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOJ21562.1"
                     /translation="MAVRPEEVTQVLEQELRGYKKELEEVGVGRVLQVGDGIARAYGL
                     RDCLSSEMLEFENGVFGIALNLEEDNVGIIILGDDRDIREGDIVRRTGRIVDVPVGEK
                     LVGRVVNAIGEPIDGKGPIVTNQRRHIEERGPGVVERQPVVEPLQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTALAIDTIINQKGQDVYCIYVAIGQKQSTVASVMKTLEDYGAM
                     EYTTIVSATASDSAPMQYIAPYSGCAMGEYFRDTKRHALVIYDDLSKHAQAYRQVSLL
                     LRRPPGREAYPGDVFNLHSRLLERAAKLSDALGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLATDLFYAGVRPAVDVGISVSRVGGKAQTKAMKTVAGGLRLDLARYREL
                     EAFTQFASDLDKATRQQLNRGAHLVEVLKQPQYQPMSVADQVVIIWAATNGYIDDLPL
                     DQVARFEQELLAFVHDRYQDIIQRISKEKAISDETRQVMEKAVREFKTQFQV"
     gene            complement(3509..4057)
                     /gene="atpH"
                     /locus_tag="PLY56_08510"
     CDS             complement(3509..4057)
                     /gene="atpH"
                     /locus_tag="PLY56_08510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOJ21563.1"
                     /translation="MIASQTVARRYAEALFVAARGEDQVNAIERDLASLSALVERTPQ
                     FLEALHNPLLPVERKQAIIHRLLGEEVQPITLRMLNLLVEKRRSEVLPYLYSLYTEMA
                     NDYRGVAQASVVSAMPLTPEEEEALVSRLAAMTGKRIELRTEVRPELIGGLRVTVGDT
                     VIDGTVVGYLRQLRGRLKEALL"
     gene            complement(4057..>4383)
                     /locus_tag="PLY56_08515"
     CDS             complement(4057..>4383)
                     /locus_tag="PLY56_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21564.1"
                     /translation="KLQREYETRMAQAEAEARDRIQDGIREGQAMRAELLAQAQQERD
                     RIIQQGHQEIREEREKMVYVVRETVANMAVDAAGKIIARSMDENTHRALVQEFLAALP
                     AGEKRA"
BASE COUNT          740 a         1512 c         1315 g          816 t
ORIGIN      
        1 cggctgatag cctacggccg agggaattct cccgaggagg gccgatacct cggaacccgc
       61 ctgggtgaac cggaagatgt tgtcgatgaa gaggagcacg tcttggcgct cgacatcgcg
      121 gaagtactcg gccatggtga gggcgctcag ggccacgcgg aggcgggcac cgggcggctc
      181 gttcatctgc ccgaagacca tcgtggtctt ctcaatgacg cccgactcgc gcatctccag
      241 ccagaggtca ttcccctcgc gcgtgcgctc gccgacgccg gcgaagacgg agaagcctcc
      301 gtgctcggtc gcgatgttgc ggatcagctc ctggacgagg accgttttcc caacgccagc
      361 gccgccgaac aggccgatct tgccaccctt cagatagggg cagaggaggt caatcacctt
      421 gatgccggtc tcgaggatct gcatctcggt gctctgctcc tcgaaggagg gcgctggccg
      481 gtggatcggg taatagtcat tccccttcac cgggcccata tcatccacgg gctcgcccaa
      541 caggttgaag acgcgtccga gtgtattccg acccacggga acggtaatcg gctgtcccgt
      601 atccctcgcc ttcgcgcctc ggggcaatcc atccgtcggc gccatggcca cgcagcgcac
      661 cgtgtcgttg cccagatcct gcgccacctc agcgatgatg cgcgaggtct ggccgttctg
      721 cggcatctcg acctcgattg cgttgagaat ctttgggagt tggcccgctg ggaagcggat
      781 atccagcacc ggcccgatga tctgaacgac gtgtcccgtc gccccgcctt gattcacctc
      841 tgccatcgaa acaactatcc cttcagcgcg tcggcgccgc ccacaatctc ggcaatctcg
      901 cgggtgatcg cggcttggcg cgcgcggttc aatgaaagcg tcaatcggtc gatcatctcg
      961 gcggcattat ccgtggcgct ccgcattgcg gtcatgcgtg cgccatgctc actcgccacc
     1021 gactccacca gtgcctggta cacctgcaca tccaggtagc gtgggagcag gctggccagg
     1081 aagcgctctg gcaccggctc gaagagatac tcctcaactc cctgcaaccg tgattctgct
     1141 gcctgcggtg gttgcagcgg caggagttgc tgcacagtcg gctgcaggcg gagcgccgag
     1201 atgaagcgcg tatagacgag gtacgcgata tcaatctcgc cgcttccgaa gagccggcgc
     1261 acctcgcggg tgatcgcctg agcatccgcc agggccacat cgcgcgaggg tagctcgaac
     1321 gtcgccccca gggtgcaccc acgctgccga aatgccgacg gccccttgcg cccaaccggc
     1381 aacaccacgg actctagatc cttatgctcg gagaggagtt ccatcgctgt gcggacgatg
     1441 ttggtgttgt agctgccgca caggccccgg tccgcggtga tcaccaccac cgccatccgc
     1501 cggggctcgc gcacctccag caacggatgc tcgattcccg aggcagcacc tgccaggtgg
     1561 cgcatcactt ccgccatctt ctcggcgtag ggccgcgccg ccccgacacg gttctgagcg
     1621 cgcttcaggc gcgctgccgc caccatctgc atcgcgctgg tgatcttctg tatgttccgc
     1681 acgacccgga tccgctgccg gatatcgcgc atgctcgcca tcgcttaacc cccggactcc
     1741 ctgctgggga tgcggtgtgc gagagagggg atgcgtggcc cggcgccctc cgggccgtga
     1801 gtgccggccc tcaggtgccg cacgttccgc tcacatcccg cgtctcccgc tcgcatcata
     1861 cctggaactg cgtcttgaac tcgcggaccg ccttctccat cacctgccgc gtctcgtcgg
     1921 agatcgcctt ctccttggag atccgctgga tgatgtcttg gtagcgatca tgcacaaagg
     1981 cgagcagctc ctgctcgaaa cgcgccacct ggtccagcgg cagatcgtcg atatacccat
     2041 tggtggcagc ccagatgatg accacctggt cggcaacgga catcggctgg tactggggct
     2101 gcttcaggac ctccaccagg tgagcgccgc ggttcagctg ctggcgcgtc gccttatcga
     2161 gatccgaggc aaactgcgta aacgcctcca gctcgcgata gcgtgccagg tccaggcgga
     2221 ggccgccggc caccgtcttc atcgccttcg tctgcgcctt gccgccaacg cgcgagacgg
     2281 agataccaac gtcaacagcc gggcgcacgc cggcgtagaa gaggtccgtc gccaggtaga
     2341 tctggccatc ggtgatcgag atcacgttgg tggggatgta agccgagaca tcgccggcct
     2401 gcgtctcgat gatcggcagc gccgtgagcg agccaccgcc gagggcatcg ctcaacttgg
     2461 ccgcgcgctc caggaggcgc gaatggaggt tgaagacgtc acccgggtag gcctcgcgac
     2521 caggcgggcg gcgcaggagc agcgaaacct ggcgataggc ctgcgcgtgc ttggagaggt
     2581 catcatagat gaccagagcg tgccgcttcg tatcgcggaa atactcgccc atcgcgcacc
     2641 cggagtaggg cgcgatatac tgcatcggcg cagagtcgct cgccgtggcg ctcacgatgg
     2701 tcgtatattc catcgcgcca tagtcctcga gcgtcttcat caccgaggca acggtcgatt
     2761 gcttctggcc gatcgccacg tagatgcagt agacgtcctg ccccttctga ttgatgatgg
     2821 tgtcaatcgc gagggctgtc ttacccgtct gacggtcgcc aataatcagc tcgcgctgcc
     2881 cccggccaat cgggatcatc gagtcgatcg ccttcagacc cgtctgcagc ggttcgacaa
     2941 ctggctggcg ctcgacgacg cccggaccgc gctcctcgat atgccggcgc tgattcgtga
     3001 caatggggcc cttgccatcg atcggctcgc caatcgcgtt caccacgcgg ccaacgagct
     3061 tctcgccgac gggcacatcg acgatgcggc ccgtgcggcg cacaatatcg ccctctcgga
     3121 tatcgcggtc gtcgccgagg atgatgatgc cgacgttgtc ttcctcgagg ttgagggcga
     3181 tcccaaagac accgttttca aactccagca tctcgctcga cagacaatcg cgcaggccat
     3241 aggcacgcgc gatcccgtcg cccacctgga ggacgcgccc gaccccaacc tcctccaact
     3301 ccttcttata tccccggagc tcttgctcca gtacctgagt gacctcttcg ggcctgacag
     3361 ccatggaacc ccccaagcag attgcagatt atcgttcgtt gaagtcaagc cgccggggct
     3421 tgctcccccg gctccgcgag acgccggtct gcggaccgcg tcccgggcac tctcacccgc
     3481 cgtgcctctt gcacgccgga accgtttatt acagcagggc ttctttcagc cggccgcgca
     3541 gctgccgcag gtagcccacc accgtgccat caatcacggt gtcgcccacg gtgacgcgca
     3601 gccccccgat cagctcgggg cgcacttcgg tgcgcaactc gatgcgcttg ccagtcatcg
     3661 ccgccagccg cgataccaag gcttcctctt cctccggtgt caacggcatc gccgagacaa
     3721 ccgacgcctg agcaacgccg cggtaatcgt tcgccatctc cgtgtagagc gagtagagat
     3781 agggcagcac ctcactgcgt cgcttctcca cgaggagatt gagcatgcgc agcgtgatcg
     3841 gctgcacctc ctcgcccagg aggcggtgga taatcgcttg ctttcgctcc accggcagca
     3901 gcggattgtg gagcgcctcc aggaattgcg gcgtgcgctc gaccaacgct gagagcgacg
     3961 cgaggtctct ctcgatggcg ttcacctggt cttcgccacg cgccgcgacg aagagcgcct
     4021 cggcataccg cctggcaacc gtctgcgagg caatcatcag gccctcttct ctcctgccgg
     4081 cagtgccgcc aggaactcct ggacgagggc gcggtgcgtg ttctcatcca tggagcgcgc
     4141 gatgatcttc ccggcggcgt ccacagccat gttggccacc gtctcgcgca ccacgtaaac
     4201 catcttctcg cgctcctcgc ggatctcttg gtggccctgt tggataatcc ggtcgcgctc
     4261 ctgttgcgcc tgcgccaaca gctccgcgcg catcgcctgg ccttcgcgaa tcccgtcctg
     4321 gatccggtcg cgtgcctcag cttccgcctg cgccatccgc gtctcgtact cgcgctggag
     4381 ctt
//
LOCUS       DAOSCV010000301         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12590_length_4379_cov_2.887835, whole genome shotgun sequence.
ACCESSION   DAOSCV010000301 DAOSCV010000000
VERSION     DAOSCV010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12590_length_4379_cov_2.887835"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /gene="ccsA"
                     /locus_tag="PLY56_08520"
     CDS             complement(<1..1546)
                     /gene="ccsA"
                     /locus_tag="PLY56_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOJ21565.1"
                     /translation="MAGFGTALLWGGLATALVAFGAYVAASRGQATRALARAAFALTF
                     VTALAACLLLLAGFVQGRYEIRYIAEYSSRDLPILYRVSALWAGQEGSFLLWALLSAI
                     LGLLLIRYSGDWETRVMPPYAATQAYLGLLLVAKSPFLLSQERLSDGRGLNPLLQDPW
                     MAIHPPMVFLGYAALAIPFVFALAALVWRDYDSWVSRALPWTLFAWVTLGAGICIGGY
                     WAYRVLGWGGYWGWDPVENASLVPWLLATALLHGTLVQRARGGLKHANLLLTSVAFAA
                     MVYGTFLTRSGVLSNFSVHSFVSPGSAVYSLLLMGVFFSLLVPLSLYATRAGEIQAKG
                     TVESTLSREFTFFLAILVFCISAAMVALGTSAPILTTILGRPTSVQPRFYNLTQFPVA
                     VLLALLVGIAPLLEWGASRPETVVRRLLPGLAVALAGAVAALALGVHRFNAWMLLLVA
                     AAAFALAVGGRIVLRGLPRRPVHIGGHLAHVGLALMLLGIVGSSVCSRTEKLSLRRGE
                     AAEALGY"
     gene            complement(1715..2098)
                     /locus_tag="PLY56_08525"
     CDS             complement(1715..2098)
                     /locus_tag="PLY56_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ21566.1"
                     /translation="MPEKEIVSTNNAPAAIGPYSQGVKAGPFLFISGQIPVDPATGEL
                     VTGSIEEQTRRVMENLKAILAAAGLEMSRVVKTTIFLTDLSHFAAVNAVYGDYFPTNP
                     PARSTVEVSSLPKGVGVEVEAVALL"
     gene            complement(2120..3061)
                     /locus_tag="PLY56_08530"
     CDS             complement(2120..3061)
                     /locus_tag="PLY56_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ21567.1"
                     /translation="MNKRDALLSVLESSKPQEYVPAAFFTHFPPEYHGGQAAVEKHLE
                     FFRFTGMDFIKIQYERKFPPVPQIRRPEDWAKIPFYDLEFYEPQLQAVEGLVKAAKSE
                     ALVLCTLYSPLMCAGHTTSHETVTAHLSEDPDAVAKGLERITESLRLFVRECIRLGVD
                     GFYHSTQGGEAGRFPHPGIFRDFIKPFDLSLMEEINQKCPFNILHVCDYHGGYDDLTP
                     FLDYPGHVVNAPLVVAGQPITGQEIARLFGRPFMGGMDRLGVLATGTEEDVRAEARRA
                     LEQAPSPFILAADCTVPPGTPWENLRAAIDEAHRARA"
     gene            complement(3260..>4379)
                     /locus_tag="PLY56_08535"
     CDS             complement(3260..>4379)
                     /locus_tag="PLY56_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21568.1"
                     /translation="IGFGYLVSTEISFTIWVTYVLERLVAVIGSAWGYREAGFPFDQE
                     QSMGAYLAIALVLLYYGRHHLAGVIRAALWSGDPEMRAYRLPFWGFWAGLAAMLLFCH
                     ALGMRLWVAGVYLGIVLCVGLVYARLRAEIGVPLIWLFPYGAPKMLLLSLFGTAPLAP
                     GGDPATLTGLAALTFLSRGFPVALAGYQIDALRAGHFVGEKPRCVAGALTLALIAGVL
                     LAFYFHLTGYYKVGAQQVRGGIWGWDMACTEFNNAINWSESPKPRDVPRCIAAGSGFL
                     TALLLQAVRSQIVGFPLHPLGFAAGNAYGRLLWGSFLLVWVIKGLVLRLGGMPLYRKT
                     VPAFLGFALGHFLTAGVVWGFLGTFGKDLYQGYIVVFG"
BASE COUNT          851 a         1417 c         1502 g          609 t
ORIGIN      
        1 ggtagcccag agcctccgcc gcctcgccgc ggcgcagtga cagcttttcg gtgcggctgc
       61 agaccgagga gccgacgatg ccaagcagca tcagggcgag ccccacatgg gcgaggtgcc
      121 cgccgatgtg gaccggccgg cgcgggagcc cgcgaaggac gatccggcca cccaccgcca
      181 gggcaaacgc ggctgctgcg acgaggagga gcatccaggc gttgaagcgg tggacaccaa
      241 gagcgagcgc ggccactgcg cccgcgaggg ccacggcgag cccggggagc aggcggcgca
      301 ccaccgtctc cggacgagac gccccccatt ccaggagcgg tgcaatcccg acgaggagcg
      361 cgaggagcac ggccaccgga aactgcgtca gattgtagaa gcgtggctgc accgaggtcg
      421 ggcggcccag gatcgtggtg aggatcggcg cggacgtgcc cagggcgacc atcgccgcgg
      481 agatgcagaa gaccaggatc gccaggaaga aagtgaactc gcgcgagagg gtgctctcga
      541 ctgtgccctt cgcctggatc tcgcccgcgc gcgtcgcgta gagcgagagt ggcacgagaa
      601 gggagaagaa cactcccatc agcaggagac tatagaccgc ggatccgggg ctgacgaagg
      661 agtgaaccga gaagttactc aagacgccgc tgcgggtgag gaaggtccca tagaccatcg
      721 ccgcgaacgc cacgctcgtc agaaggaggt tggcgtgctt cagcccgccg cgcgcacgct
      781 gcacgagcgt accatggagc aaggccgtcg cgaggagcca cgggacgagt gacgcgttct
      841 ccacgggatc ccagccccag tagccacccc agcccagcac gcgatatgcc cagtacccgc
      901 cgatgcagat acctgccccc agcgtcaccc aggcgaagag agtccaggga agcgcgcgcg
      961 acacccagga gtcgtagtcg cgccacacga gcgccgccag cgcgaacacg aacgggatgg
     1021 cgagcgcggc atagcccagg aagaccatcg gggggtggat cgccatccag gggtcctgga
     1081 ggagcgggtt cagcccgcgt ccatccgaaa gcctctcctg ggagaggaga aacgggctct
     1141 tggcgaccag aagcagcccg aggtacgcct gcgtcgcggc atagggaggc atcacgcgcg
     1201 tctcccagtc gccgctatag cggatcagca gcagcccgag gatggcgctc agaagtgccc
     1261 acagaaggaa gctgccctcc tggccagccc acagcgccga gacgcgatag aggatgggaa
     1321 gatcgcgcga tgagtactct gcgatgtagc ggatctcgta gcgcccctgc acaaaccctg
     1381 ccaggagcag gaggcacgcg gccagcgccg taacaaaggt gagcgcgaac gccgcgcggg
     1441 cgagcgcccg cgtcgcctgc ccgcgcgaag cggcgacgta ggcgccaaaa gccaccagcg
     1501 cggtcgcgag tcctccccag aggagtgcgg tgccaaaacc ggccatgcgg gaaagcccct
     1561 tcaagtacca ccgcggctgg cctcagcacc tcttgccagc cgccagcaca ccctgccatg
     1621 gtaccatccg gggcacgcct cgtctatcag tcattgcccg tatcaggctc ggggttttcc
     1681 cgcagggtca ggtgcgtgtt tctccccggc cggctcagag aagcgccacg gcttccacct
     1741 caacgcccac gcccttgggc aaggaagaga cctccacggt ggagcgtgca ggcggattgg
     1801 tggggaagta gtcgccgtaa accgcattga cggccgcgaa gtggctcaga tcggtgagga
     1861 agatggtggt tttcaccacc cggctcatct ccagacccgc ggcggcgagg atcgccttca
     1921 ggttctccat cacgcgcctc gtctgctcct cgatggagcc ggtgaccagc tcgccggtcg
     1981 ccgggtccac cgggatctgg ccggagatga agaggaatgg cccggccttc actccctgcg
     2041 agtaggggcc gatcgccgcg ggggcgttgt tggttgagac aatctccttt tcgggcatga
     2101 tctactctcc tcggtctctc tatgctcgcg cgcggtgcgc ctcgtcgatg gcggcgcgca
     2161 ggttctccca gggggttccg ggcggcacgg tgcaatccgc ggccaggatg aagggcgatg
     2221 gcgcctgctc cagagcgcgg cgggcctccg cgcggacatc ctcttccgtt cccgtggcca
     2281 gaacgcccag gcgatccatg ccccccatga agggccggcc gaagaggcgc gcgatctcct
     2341 ggccagtgat cggctggccg gccacgacca gcggcgcgtt gactacatgc cccggatagt
     2401 cgaggaaggg cgtcaggtcg tcatagccgc cgtgatagtc gcacacgtga aggatgttga
     2461 aggggcactt ctggttgatc tcctccatca gcgagaggtc aaacggcttg atgaagtcgc
     2521 ggaagatgcc ggggtggggg aagcgcccgg cctccccgcc ctgggtagag tggtagaagc
     2581 catccacgcc cagccggata cactcgcgca cgaagaggcg caggctctcg gtgatccgct
     2641 cgaggccctt cgccaccgca tcgggatcct cgctcaggtg cgcggtgacc gtctcgtgac
     2701 tggttgtgtg cccggcgcac atcagcggcg agtagagtgt gcacaggaca agcgcctcgg
     2761 atttagccgc cttgaccagc ccctcgacag cctgaagctg cggctcgtag aactccaggt
     2821 cgtagaaggg gatcttcgcc cagtcctcgg gccggcggat ctggggcacc ggcgggaact
     2881 tccgctcgta ctggatcttg atgaaatcca tgccggtgaa gcggaagaac tcgaggtgct
     2941 tctccacggc ggcctgcccg ccatggtatt ctggggggaa gtgcgtgaag aacgccgccg
     3001 gcacgtactc ctgcggcttg ctactctcca gaacgctaag caaggcatcg cgcttattca
     3061 tgccactctc tcccacggga aaaacgcccg cgccgccggg ttggatggcg cgcgggcgta
     3121 ctatgatttc aggatcgacg ggtcggcttc cttttcacca cactgcgcgg ggaaatggcg
     3181 gaccgagggt tcctcggccc ttcatcagtg gtaacgcgct ctctcctggg gccaccgcgc
     3241 gcttgcgtgc cttggattcc tacccaaaga cgacgatgta gccctgatac aggtccttgc
     3301 caaaggttcc aagaaagccc cagacgacgc cggctgtgag gaagtgcccg agggcgaagc
     3361 cgaggaaggc gggcacggtt ttccgataga gtggcatgcc gccgaggcgg aggaccagcc
     3421 ccttgatcac ccagaccagc aggaacgagc cccagagaag ccggccatac gcgttgccgg
     3481 cggcaaagcc gagggggtgc agcggaaagc cgacgatctg cgaccgcaca gcctggagca
     3541 gcagcgccgt gaggaagccg gagccggcgg caatgcagcg gggcacgtcg cgcggcttgg
     3601 ggctttctga ccagttgatg gcgttgttga actcggtgca ggccatgtcc caaccccaga
     3661 ttccgccgcg tacctgctgg gcccccacct tatagtagcc cgtgaggtgg aagtagaagg
     3721 cgaggaggac gccagcaatc agggcgaggg tgagcgcccc ggccacgcac cgtggcttct
     3781 cgccaacgaa gtggccggcg cggagcgcgt cgatctgata gcccgccagg gcaacgggga
     3841 agccgcgcga gaggaatgtg agcgccgcga gtccggtcag cgtcgcggga tcgccgccgg
     3901 gcgccagggg ggccgtgccg aagagggaga ggaggagcat cttcggcgca ccgtagggga
     3961 agagccagat cagggggacc ccgatctccg cgcgcaaccg ggcgtacacc agtcccacgc
     4021 agaggacgat ccccagatag acgccggcaa cccagagccg catcccaagc gcgtggcaaa
     4081 agaggagcat cgctgccagg ccggcccaga agccccagaa ggggaggcgg taggcacgca
     4141 tctctggatc gccgctccag agagccgcgc ggatcacgcc ggccagatga tgccggccat
     4201 agtagaggag caccagcgcg attgccaggt aggcgcccat gctctgctcc tggtcgaagg
     4261 gaaaacctgc ctcgcgatac ccccaggcgc tgccgatcac cgccacgagc cgctccagga
     4321 cgtaggtgac ccagatcgtg aagctgatct ccgtggagac gaggtagccg aagccgatc
//
LOCUS       DAOSCV010000302         4377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12602_length_4377_cov_3.951874, whole genome shotgun sequence.
ACCESSION   DAOSCV010000302 DAOSCV010000000
VERSION     DAOSCV010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12602_length_4377_cov_3.951874"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(712..1386)
                     /locus_tag="PLY56_08540"
     CDS             complement(712..1386)
                     /locus_tag="PLY56_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21569.1"
                     /translation="MRKQGFTLIELLVVIAIIAILAAILFPVFARARENARKATCQNN
                     CKQLGMAMLQYVQDYDETFPGRAVGVSPHDTASGQTVCWVGLVFPYVKNTGVFLCPSY
                     ASRYTQHAWQGSTRIDIQGNMGYNFCGVGGSTNASCSMAQITTPAEVPLIGDAVCAGL
                     KSTGTDPRNCLYIGPGTNTTGVYPNEVHPRMSVHTDGINLVFCDGHVKWFKASQVPRG
                     SFWARK"
     gene            1742..3556
                     /gene="aspS"
                     /locus_tag="PLY56_08545"
     CDS             1742..3556
                     /gene="aspS"
                     /locus_tag="PLY56_08545"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOJ21570.1"
                     /translation="MPASLRRDHHCGELNLNHVGREVTVMGWAHHVRDLGGLIFIELR
                     DRSGIVQVAFSPQEAPEAHALAQEVRSEFVLGVRGVVRRRPAGTENPNLPTGEVEVYG
                     SHLEILNTCQPLPFPVAEEVDVDETTRLKYRYLDLRRAPMQRMLELRHRVTSAVRSFL
                     NSQGFWEVETPLLMKSTPEGARDYLVPSRVHPGKFYALPQSPQLLKQILMVAGVEKYY
                     QIARCLRDEDLRADRQPEHTQIDLEMSFVTRDDIFDVAERMWQYVFREALGEEISIPF
                     RRMTYAEAMARYGSDKPDLRYGMELVDISSLVAGTEFKVFSGAVASGGLVKGLNARGC
                     ASFARREVDELVEVSKTFGAKGLAYFYVEESGLRGPIAKFFTTEQLEAIRAALDGKPG
                     DLLLFVADQPDVTNTALGRLRVHLAKQLNLADPAQRRFVWIIDFPMFEWKPEEKRYDF
                     MHNPVSAPHPDDLHLLEEGWKSEAEPGSPEHPWTRIRANQYDLVLNGSEVASGSIRNH
                     RADLQERVFEILGISREKAHERFGFLLDAFQYGAPPHGGIAPGLDRMVAIMAGAESIR
                     DVIAFPKTASATDLMMDAPSEVDPAQLEELHIRVVPPK"
     gene            3584..>4377
                     /locus_tag="PLY56_08550"
     CDS             3584..>4377
                     /locus_tag="PLY56_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HOJ21571.1"
                     /translation="MHQGGHAAFIFSEAFMEYDFGPLHPLRPERLRLLRERLQADGVL
                     DSPHVRLVEAQPATEEEVLAVHTREYYDAVAALSSGHRVVKREEYGFGGLDNLPFPGM
                     LEASLLYTGASLQAARLVSRGEVPRAMNPAGGLHHAMPGRASGFCIFNDPAVAIADLL
                     RRHERVLYLDIDAHHGDGVQAVFYESPRVLTLSLHESGRTLFPGTGSPEEIGTGAGEG
                     YSVNAPLMAGTGDDTFLRVFDALVPPLVEWFKPEVIVAQLGIDTHHA"
BASE COUNT          755 a         1458 c         1416 g          748 t
ORIGIN      
        1 tgaattagat gagtgtcctc gggtgcgagg gaaggaggtg ggtggcatgc aggtaatgaa
       61 atgcccagag tgtggagaga gaaaccccgt ggctcgcgag tattgcggag catgcggcga
      121 gtatctatgg ccacgggctg aatgccagca caccggtcac gcgcggcttg ccgtctcgcc
      181 cgcgcagtct cctcccgctg gactgcgtgc gaccgccgcc cggttgttgc ggaggctgtt
      241 cgggccccat tctcccgatg cttgatgcca ggggcgctgt ctggccggct ccttgcgggt
      301 agtaccgcac ctttcctgga gcgcctttcc gggcgcgagg gcggcatggc aaggagccgg
      361 cgggcagaca gcaggcgcgt ctgactgttc gaaatcgccc aaggcgtgtg gttcccaccc
      421 ccaagacaat cgcagacacc tcacaccaat ccgtcgcggc cacaatcgac cgccatccag
      481 caccgcgggc ttccctggtg gcggattcga ggccagtcgc ctgcttgata cccgggcgcc
      541 ccccttactg gcgcctcgat ggcggaatcg accagcggcg ccgtctccga catccgcaac
      601 aggcgccggc ctcgccgtgc caaccaccac ccggccatcg ccacccggga gcactctccc
      661 agcgtccgga gggagccagc gccactcccc cgctggctgg aagcaacgcg gctacttgcg
      721 cgcccagaag ctgccgcgtg gcacctggct cgccttgaac cacttcacgt gaccatcgca
      781 gaacaccagg ttgatcccgt ccgtatgcac ggacatgcgc gggtggacct cattcggata
      841 gacacccgtc gtgttggtgc ccggaccgat gtagaggcag tttcgggggt ctgtgcctgt
      901 gctcttgagg cccgcgcaca ccgcgtcgcc gatcagcggc acctcggccg gcgtggtgat
      961 ctgcgccatg gagcaggacg cgttcgtcga gccgccgaca ccgcagaagt tgtagcccat
     1021 gttcccctga atatcgattc gggtggaacc ttgccaggcg tgctgggtgt agcgactcgc
     1081 gtagctcggg cacaggaaga cgcccgtatt cttcacgtag gggaatacca gtccgaccca
     1141 gcagaccgtc tggcccgacg ccgtgtcgtg gggcgagacg ccgacggcgc gccctgggaa
     1201 cgtctcgtca tagtcctgaa cgtactgcag catcgccatg ccaagctgct tgcagttgtt
     1261 ctggcaggtc gccttgcgcg cgttctctcg ggcgcgcgcg aaaaccggga agaggatcgc
     1321 cgcgagaatc gcgataatgg cgatcacaac gagcaactcg atcagggtga aaccttgctt
     1381 cctcatctcc gtccccctct ctgtcacccg gaagtggccc gctcagatgg caggcgggcc
     1441 cccttcctgc aggcgctttc gcgctcacca aggagaactc cttctggacg aatgcgcccc
     1501 accgcgggcc ccgattccaa ggaaggccgc acagccaggg gggcccgcac atccccatcc
     1561 gcgtccttcc atatcatccg ctgtatcggc acccgcgtgg ggtcaggttt cgttgacaaa
     1621 acccacccca tccggtacaa tgaacgcaag aatactatgc cggcacggca acagaagcgc
     1681 gcgactgctt gggcgcgttg ggagggcgcc gcccgccggc tcttgtgatg aggaacccag
     1741 catgccagca tctttgcgca gggatcacca ctgcggcgaa ctgaacttga accacgtcgg
     1801 ccgcgaggtt accgtgatgg ggtgggcgca ccatgtgcgc gaccttggcg gcctgatctt
     1861 cattgaactg cgcgaccgca gcggcatcgt ccaggtcgcg ttcagccccc aggaggcacc
     1921 cgaggcacac gccctcgctc aggaagtgcg cagcgagttc gtccttggcg tccgcggggt
     1981 ggtccgccgc cgcccggcag gcacggagaa ccccaatctg cccacgggcg aggtggaagt
     2041 ttacggctct caccttgaga ttctgaacac gtgtcaaccc ctccccttcc ccgttgccga
     2101 ggaggtggat gtcgatgaaa cgacgcggct gaagtatcgc tacctcgacc tgcgccgcgc
     2161 gccgatgcag cgaatgctgg agttgcgcca ccgcgttacc tccgccgtcc gcagcttcct
     2221 caacagccaa gggttctggg aggtcgagac gccgctgttg atgaaaagca cgccggaggg
     2281 cgcccgcgac tatctggtac ccagccgcgt ccatccgggc aagttctacg cgctgcccca
     2341 gtcgccacag ctgctgaagc agatcctgat ggtcgcgggc gtggaaaagt attaccagat
     2401 cgcccgctgc ctccgcgacg aggacctgcg cgccgaccgc cagccggaac acacgcagat
     2461 cgatctggag atgtcgttcg tcacccgtga cgatatcttc gacgtcgcgg agcggatgtg
     2521 gcagtacgtc ttccgcgagg cgctgggcga agagatctcc atcccgttcc ggcggatgac
     2581 ctatgccgag gcgatggccc gctacggcag cgataagccc gacctgcgct acggcatgga
     2641 gctggtggat atctcctcac tcgtggcggg caccgagttc aaggtcttct ccggtgccgt
     2701 cgcctcgggt ggcctcgtga agggcctgaa cgcccgcggc tgcgccagct tcgcgcgccg
     2761 agaggttgac gagctggtgg aggtgtccaa gacgttcggc gcgaagggcc tcgcctactt
     2821 ctacgtcgag gagagtggcc tgcgcggccc catcgcgaaa ttcttcacga ccgagcagct
     2881 ggaggcgatc cgcgccgccc tggatggcaa gccgggcgac ctgcttctct ttgtcgccga
     2941 ccagccggac gtcaccaaca ccgccctggg ccgcctgcgc gtgcatctgg ccaagcagct
     3001 caacctggcc gaccccgcac agcgccgctt cgtctggatc atcgacttcc cgatgttcga
     3061 gtggaagccc gaggagaagc ggtacgactt tatgcacaat ccggtgagcg ccccgcaccc
     3121 ggatgatctc cacctccttg aggaggggtg gaagagcgaa gccgagcccg gcagcccgga
     3181 gcatccctgg acgcgcatcc gcgcgaatca gtatgacctg gtgctcaacg gcagcgaagt
     3241 cgcctccgga agcatccgaa accaccgggc cgacctccag gagcgcgttt ttgagattct
     3301 gggaatcagc cgcgagaagg cacacgagcg gttcggtttc ctgctggacg ccttccagta
     3361 tggagcgccg cctcatggcg gaatcgcccc agggctggat cggatggtgg ccatcatggc
     3421 cggcgcagag tcaatccgcg acgtcatcgc cttcccgaag acggccagcg caaccgatct
     3481 catgatggat gcgccctcgg aagtggatcc ggcacagctt gaggagcttc atatccgggt
     3541 ggtgcctccg aaatagaagc cgggcaagcg ctcgggagga gccatgcacc aaggtgggca
     3601 tgcggcgttt atcttctccg aggcattcat ggagtatgac ttcgggccgc tccacccgct
     3661 gcgcccggag cgcctgcgcc tcctgcgcga gcggctccag gccgatgggg tgctagacag
     3721 ccctcatgtc cgcctggtgg aggcgcagcc ggccaccgag gaggaggtgc tggcggtcca
     3781 cacccgggaa tactacgacg cggtggcggc gctcagctct ggccaccgcg tcgtgaagag
     3841 agaggagtat ggcttcgggg ggctggataa cctcccgttt cccgggatgc tggaggcttc
     3901 cctgctctat acaggggcgt cgttgcaggc agcgcgcctc gtgagccgcg gagaggtgcc
     3961 gcgggcgatg aaccccgccg ggggcctgca ccacgccatg cccgggcgcg ccagcgggtt
     4021 ctgcatcttt aacgacccgg cggtcgccat tgccgatctc ctgcggcggc acgagcgagt
     4081 gctctatctg gacatcgatg cgcatcacgg cgatggagtc caggcggtct tctatgaatc
     4141 gccgcgcgtg ctcaccctct cgctgcacga gtccgggcgg acgctcttcc cgggcaccgg
     4201 ctccccggag gagatcggca cgggcgcggg cgagggctac agtgtcaacg cgccgctgat
     4261 ggctggcacg ggcgacgaca ccttcttgcg cgtctttgac gccctggtgc ccccgctggt
     4321 ggagtggttc aaaccagagg tgatcgtcgc ccagcttggg atcgatacgc accatgc
//
LOCUS       DAOSCV010000303         4375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12606_length_4375_cov_3.497917, whole genome shotgun sequence.
ACCESSION   DAOSCV010000303 DAOSCV010000000
VERSION     DAOSCV010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12606_length_4375_cov_3.497917"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PLY56_08555"
     CDS             <1..1297
                     /locus_tag="PLY56_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21572.1"
                     /translation="VAREASAGVRRLRNHPCLALWCGNNECEMDTEANAVWRGKRLFH
                     DTIARIVRQLDPGRVYWPSSPYGGEIAHSSEHGDYHGEPWFHVAKLGPDHWRECLEKD
                     RGLFMSEFPTQGPPEVESIRRFMPAGSLFPVSGAAWEEHLENNAQRQERTGYTSRELL
                     LRNIRAFMGEPETIEEFARLGGILQGEFLASQIEFYRRHKGTMGGALFWMFGEAWPAV
                     SYGVVDHYLRPKPAYYYVKRACAPVLLSFDHQGGEAEAWIVNDRLEPVQGLLSVGRVA
                     GGVLAGPMERLPVTVADNSIQRVWSGPAADDPAEEWLIGMLDMGGRVVGRASHFFSLP
                     RQVGFPAPAVHLQQEYLDGILIIAVAGDTYTRAISLEGLPDQARPDDNYFDLFPGEVR
                     RIGVAGLSPEEAAQVRVITGQSVTPARRGQPAPALAGAR"
     gene            complement(1493..2422)
                     /locus_tag="PLY56_08560"
     CDS             complement(1493..2422)
                     /locus_tag="PLY56_08560"
                     /inference="COORDINATES: protein motif:HMM:NF019127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HOJ21573.1"
                     /translation="MRITANRLGGLLALTFLFLTAPAHSQAPAPAAASPTPPAYRDVE
                     RILQAPGRMEEGAFVVHAPRTDLQVTMGQPLPPGFDLTSFIAWTFRPEGYLTSGYLVV
                     REEEMNPVVSELRAAGIRIAALVRHMIGEQPRIMYVHFQGRGSGPTLAQGIRRALGRT
                     GTPLPTPPGALALPPPAGLDVNRIQLALRRSGIIEGGVLKVTQGRPGVRLQGVELPPG
                     MGPFSVAYFTGTDANALVGGEVVLTEREIDRAIDTFRQAGFLVAAQHIHLVGEEPKLY
                     FLHYSGTGPAETLARVVRSVFDQVRGPAVGSRS"
     gene            complement(2748..3188)
                     /locus_tag="PLY56_08565"
     CDS             complement(2748..3188)
                     /locus_tag="PLY56_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21574.1"
                     /translation="MRFISTYVHGIIDYAAAAALALAPNVFRFARLGGAPVRTLRGLS
                     VFAVLYSVFTDYEWGAVKVLSMRTHLKIDAAFGLFLLAAPRALKFAGLDARARRTYEG
                     FGLFSLAASLLTKTEVPCPSVVCMRSEDEEAVPARPAQPAAGTG"
     gene            complement(3800..>4375)
                     /locus_tag="PLY56_08570"
     CDS             complement(3800..>4375)
                     /locus_tag="PLY56_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ21575.1"
                     /translation="KRPPVEDTRKKEIYALVERYGLSEAARRSGTPVSTINSWIRRDR
                     AKGAPVPESVTARASVQERYRIVLDARQRGVTRAARDSSTAINTILRWEEEFVQGKHP
                     DYPLPPRQKQHPEDLVQQDGAALRAARLSAGMTQRAVAAAAGIPQPSLVRYEGEYVRC
                     PESVLRRLAEVIGVPYEALLAEPAGDEEKPA"
BASE COUNT          684 a         1478 c         1540 g          673 t
ORIGIN      
        1 ggtggcgcgg gaggcctcgg cgggggtgcg ccggctgcgc aaccatccct gcctggcgct
       61 ctggtgcggc aacaacgagt gcgagatgga tacggaggcg aacgccgtgt ggcgagggaa
      121 acgcctcttc catgacacga tcgcgcgcat cgtccgccag ctcgaccccg gacgggtcta
      181 ctggccctcc agcccctacg ggggagagat cgcccactcg tctgagcatg gcgattacca
      241 tggcgagccc tggttccacg tcgccaagct cgggcccgac cactggcgcg agtgcctgga
      301 gaaggaccgt gggctgttca tgagcgagtt cccgacccag ggcccacccg aggtcgagtc
      361 cattcgccgc ttcatgccgg caggcagcct cttccccgtg tccggagcgg cctgggagga
      421 gcacctcgag aacaacgcgc agcgccagga gcgtaccggc tataccagcc gtgagctgct
      481 gctgcgcaac atacgcgcgt tcatgggcga gccggagacc atcgaggagt tcgcccggct
      541 agggggcatc ctccaagggg agttcctggc ctcgcagatc gagttctacc gccggcacaa
      601 gggcaccatg ggcggcgccc tcttctggat gttcggcgag gcgtggccgg ccgtcagcta
      661 cggcgtggtg gaccactacc tgcggcccaa gccggcctac tactacgtga agcgcgcctg
      721 tgccccggtc ctcctctcct tcgaccacca gggcggggag gcggaggcgt ggattgtgaa
      781 tgaccggttg gagccggtgc agggcctgct ttcggtgggc cgcgtggccg gcggagtgct
      841 tgccggcccg atggagcgcc ttccggtcac ggtggcggac aacagcatcc agcgcgtctg
      901 gtccggcccg gcggcggatg atccggccga ggagtggctc atcggcatgc tcgacatggg
      961 gggccgggtg gtggggcggg ccagccactt cttttccctg ccgcgccagg taggcttccc
     1021 ggcgccggcg gtccacctgc agcaggagta cctggacggc atcctcatta tcgcggtcgc
     1081 cggcgatacc tacacgcgag ccatttcgtt ggaggggctt cccgaccagg cccgtccgga
     1141 tgacaactac ttcgacctct tccccggtga ggtgcggcgg atcggcgtgg ccggcctgag
     1201 cccggaggag gcggctcagg tacgcgttat cacagggcaa tccgtcactc ccgcacgtcg
     1261 tgggcaaccc gcaccggcgt tggccggcgc gcggtgacgt gaaggtttgc ggagatgccc
     1321 ctgtggacgt ggtcgccgga agcccgctcg ccttggccgg cgcggcaggg aggccctacg
     1381 ccacccgcca cctcggcggg tgggtcatcg ggtataggag aggctccggg aggtccggag
     1441 ccttcacggt ttgggcgatg cgacctctac cggcgagagg cgatccttcc cactacgacc
     1501 ggctaccgac ggccgggcct ctcacctggt cgaagacgga gcgcacgacg cgcgccagcg
     1561 tctccgccgg cccggttccg gaatagtgca ggaagtagag cttcggctct tcgccgacga
     1621 ggtggatatg ctgcgcggcg acgaggaagc cggcctgccg gaaggtgtcg atagcgcggt
     1681 cgatctccct ctcggtcagc acgacctcac cgcccacgag ggcgttggcg tccgtgcccg
     1741 tgaagtaggc gaccgagaac ggccccatcc cgggcggcaa ttcgaccccc tgcagccgca
     1801 caccggggcg cccctgcgtc accttcagca caccgccttc gatgatgccc gaccggcgca
     1861 aggcaagctg gatgcggttc acgtccagcc ccgctggggg cggcagggcg agcgcaccgg
     1921 gcggtgtcgg caggggggtg ccggttcgtc cgagagccct gcggatacct tgagcgaggg
     1981 tggggccact cccacggccc tggaagtgca cgtacatgat tcgcggctgc tcgccgatca
     2041 tgtggcggac gagtgcggca atccggatgc cggccgcccg caactccgaa acgaccgggt
     2101 tcatctcctc ctcacgcacg accaggtagc cggaggtcag gtatccctcc ggtctgaacg
     2161 tccacgcgat gaacgaggtg aggtcgaacc cgggcgggag cggctggccc atcgtcacct
     2221 gcagatcggt gcgtggggcg tgtactacga aggccccctc ttccatcctt ccgggggcct
     2281 gcagtatccg ctccacgtct cggtatgccg gtggcgtcgg tgacgccgct gccggggccg
     2341 gcgcctggga gtgcgccggt gccgtgagga agaggaacgt gagggccagc aggcccccaa
     2401 gcctattcgc tgtgattctc atcggcagat ctcctatggg tggttggcga aggtcgtcgt
     2461 ctgggtcctg gaacgacgtg acctatcgcg tttggcgacc ggaccgctgc cgggtgtgtc
     2521 ggcgcaacac aagacgcttc cacctccgcg aggcccgacg ccaatcaagg caggtactac
     2581 ccggtctccc cggcagcaaa cgccgggcag cgagtgggcg cacaggagtg cttcgcggtc
     2641 gcccgacctc gcaccgcggc gtctggcccg tggagcacgg tagcgcgcca gggcgcagcc
     2701 tgccctctgc catgatccgt gtgagacagc aagaggcacg gcgcgggtca gcccgtgccc
     2761 gccgccggct gggccgggcg ggccggcact gcctcttcat cctcggagcg catgcacacc
     2821 acggacggac acggcacctc ggtcttcgtg aggagcgacg cggcgagcga gaagagcccg
     2881 aagccctcgt aagtgcgccg agcgcgcgca tccagcccgg cgaacttgag tgctcgcggc
     2941 gctgccagca gaaagaggcc aaaggccgcg tcgatcttca ggtgggtgcg catggagagc
     3001 accttgactg ccccccactc gtagtccgtg aagacgctgt agagcacggc gaacacactc
     3061 aagcctctca gagtgcgcac cggcgcgccc cccagccgcg caaagcggaa gacgttcggg
     3121 gcgagcgcta gcgccgccgc tgcggcgtag tcgatgatgc cgtgtacata ggtcgagatg
     3181 aaccgcatga cacctcctga tcgctgccgg tccaggcccg ggcccagcgg aagcgccgca
     3241 aagcggcggg cggaatcccg gctccgtgct taaccggcgc cggggcctgc cctcgtttcg
     3301 ttcgatgtcc gcctgctgct cgcatcaggt gctccgggca tgtctcatcc tcacggcccc
     3361 ttacggccga gggataccat cacgatgctt ggccgacagc tccgggaccc tcctgtctcg
     3421 gggttcccgc cgtcagcgcg gcgcaaaccg cgagtgagca catccaactg ccggggcgaa
     3481 ggtgatccga gaggactacc agaagcggtt cccggaagag gccaccggct ccgcgggcat
     3541 acctcgtggc gagagggtac ggtcaggaat aacccagaga caggcggcgc acgtctccta
     3601 tcacaacgcg tggcgtaatg ccccaggagg gcacgatact cgcgacattc gttcctgagc
     3661 cgatccctag cgccctgcta cgtgccatct cggccgatac ccatccagcg gcagctcttc
     3721 ccacacttgc tctactccat agaacagcac cagagcagcg cccactacgc cttgtgccag
     3781 gcccggggca tagtccctct catgccggct tctcctcatc acccgccggc tcggcgagta
     3841 gggcttcata gggcacaccg atgacctcgg cgaggcggcg gaggacgctc tcagggcagc
     3901 ggacgtactc gccctcgtag cggacgagcg acggctgggg gatgccggcc gcggcggcga
     3961 cggcgcgctg ggtcatgccg gccgagagcc gggcggcacg gagggcggcg ccgtcctgct
     4021 gcaccaggtc ctcggggtgc tgcttctggc gcggcggcag gggatagtcg gggtgcttgc
     4081 cctggacgaa ctcctcctcc cagcggagga tggtgttgat ggcggtgctg gagtcgcggg
     4141 cggcacgggt gacgccgcgc tgccgggcat cgaggacgat gcggtagcgc tcctgcaccg
     4201 aggcgcgcgc ggtgaccgac tccggcaccg gcgcgccctt ggcacggtcg cgccggatcc
     4261 aggagttgat ggtggagacg ggcgtgccgg agcggcgcgc ggcctcggag aggccgtagc
     4321 gctcgaccag ggcgtagatc tccttcttgc gcgtgtcctc gaccggaggg cgctt
//
LOCUS       DAOSCV010000304         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12623_length_4371_cov_2.604032, whole genome shotgun sequence.
ACCESSION   DAOSCV010000304 DAOSCV010000000
VERSION     DAOSCV010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12623_length_4371_cov_2.604032"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="PLY56_08575"
     CDS             <1..1209
                     /locus_tag="PLY56_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ21576.1"
                     /translation="AGKPVKKFIEAETQQQAAERLRAAGLRVTEIKEAGKFSMAALNA
                     ALQKMGGVRPQSLVVFSRQFAVMINAGIPVIRCLDVLENQADDPVFAPIISRVKSDVM
                     AGKTLTEALLSHPQAFSPLFCSMVRAAEVGGILDIVLNRLAAFLEKEAEIRSKVKSAM
                     MYPTVIFLFAIAVTTGLFLFVLPTFKKMFTEISIDGKPLELPFLSNLIFMVGDLIKAY
                     FFIPVGLVIGGVIAYRSLQKTEKGQRIIDGAKLRLPIVGDLIRKIAISRFSRTFGTLT
                     QSGVPIMQALEIVADTAGNTIVRDAVLAARLSIREGQRLSPPLAASGVFPPMVTQMIE
                     IGEEAGKVSEMLDKIADFYDTEVDATIKGLTSMIEPLMIMFVGSIVGLIAIAVLMPIP
                     KMQEALRKTK"
     gene            1412..1813
                     /locus_tag="PLY56_08580"
     CDS             1412..1813
                     /locus_tag="PLY56_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21577.1"
                     /translation="MMYVRERKRGGFTIIELLIVIAIIAILAAVAIPALLRAQKSGKE
                     SAALQVLKSLHNAEQTFKVRSTANPKTFGTLDELKADGSSPVGEDGVEPASGYTFASE
                     GVTTDTYTIIATPPKTDMITYTLNETGAVTP"
     gene            1951..3009
                     /locus_tag="PLY56_08585"
     CDS             1951..3009
                     /locus_tag="PLY56_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HOJ21578.1"
                     /translation="MRACPLFELQKAQGAVFRPHAGFSVPAHYGDPDTECAVARKSCV
                     VTDLSYCGKLSIRGEDRGAVLDRALVGDVTSLRAGCGLHTLALTPTGRILADMVIYAL
                     DDWHLVELDLENVPRVAGQLLQLDDDADVEVQDLSEAWGLLSLQGPESADALAYLGVG
                     IPPPSCIRTAVLAGHPILVAERRRASAPGFDLFAAAGALIDCWNSILERGVLPAGLDA
                     LNALRIEAGIPWAGTELDEQVTPTEAGLEAALSSIKEGYIGHAAVVRARRRPPARRLY
                     RLVIETELAPTPGAPVYVASRVVGKVTSAARCSAGGGAVALAFLKRDWNKDGAPVMVE
                     VDGGKCPARVMHLPAMKG"
     gene            3017..3841
                     /locus_tag="PLY56_08590"
     CDS             3017..3841
                     /locus_tag="PLY56_08590"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ21579.1"
                     /translation="MVIRDRNAADGARGRRGSGSGAILRGLIWPVLVLCSLGFLAAGR
                     LQRGRGWHEISPGMEYRRLEVQASDLGDTADLHVLRVDLKHYRIRLLRASKAPAASIR
                     ELATQSRAAAAINGGFFDEELRPLGLRVTGGKELRPLRRANWGVFVIRNGKPAILHAR
                     DYRPEGVTEALQCGPRLVVNGEVTRLKKQYARRACIGIDRAGRVLLAVTNGSTMDATY
                     LARVLRAPEPKSGLGCVNALNLDGGGSAQLYVDHHGFRLDLPGAWGVPDGLGVFPR"
     gene            4165..>4371
                     /locus_tag="PLY56_08595"
     CDS             4165..>4371
                     /locus_tag="PLY56_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21580.1"
                     /translation="MDASTWSDGSSPRRVMIVDDEDLVRASLADMVELSGYQVCGQAA
                     NGYEAARLIGSAHPDIVLMDIMMPE"
BASE COUNT          759 a         1352 c         1451 g          809 t
ORIGIN      
        1 gcgggcaagc cggtcaagaa gttcatcgag gccgagacgc agcagcaggc ggccgagcgg
       61 ctgcgcgctg cgggcctgcg cgtgacggag atcaaggaag ccggcaagtt ctccatggcc
      121 gcgttgaacg ccgccctgca gaagatgggg ggcgtccggc cccagtcgct tgtggtcttc
      181 agccggcagt tcgcggtgat gatcaacgcc gggattcccg tcatccggtg tctggacgtt
      241 ctggaaaacc aggccgacga cccggtcttc gccccgatca tctcgcgagt caagtcggat
      301 gtcatggccg gcaagacgct caccgaggcg ctgttgagcc accctcaggc cttctctcct
      361 ctcttctgca gcatggtgcg tgcggcggaa gtgggcggca ttctcgatat cgtcctcaat
      421 cgcctggcgg cgtttctgga gaaggaggcg gagatccgct cgaaggtgaa gagcgcgatg
      481 atgtatccga ccgtcatctt cctcttcgcc atcgccgtga ccacgggtct cttcctcttc
      541 gtgctgccca ccttcaagaa gatgttcacc gagatcagca tcgatggcaa gccgctggag
      601 ttgccattcc tcagcaacct gatcttcatg gtgggcgacc tcatcaaggc ctacttcttc
      661 attccggtgg ggctcgtgat cggcggcgtg atcgcttacc gatccctgca gaagacggag
      721 aagggccagc gcattatcga tggcgcgaaa ctgcgcttgc ccatcgtggg cgacctcatc
      781 cgaaagatcg ccatctcgcg cttctcgcgt acctttggca cgctgacgca gagcggagtg
      841 ccgatcatgc aggccctgga gatcgtcgcc gatacggccg gcaacaccat cgtgcgcgat
      901 gccgttctgg ccgcccgact gagcattcgc gaaggccagc gcctcagccc gccactggct
      961 gccagcggcg tcttcccgcc catggtgacc cagatgattg agatcggcga ggaagctggc
     1021 aaggtgagcg agatgctcga caagatcgcc gacttctacg atacggaggt ggatgcaacg
     1081 atcaaaggcc tcacttcgat gatcgaaccg ctgatgatca tgtttgtcgg gtccatcgtt
     1141 ggcctgatcg ccatcgccgt gttgatgccc attcccaaga tgcaggaggc gctgcgcaag
     1201 acaaagtagc gccaccgagt atgtgccgag aaacagctgc ctgcggatcc ccgttccgca
     1261 gagaaaggag actggcccta tagaaagcgc aggtagcaac cgataatcag gattagaggg
     1321 caagtgccgg cagatccggg cctaagccct cccccttccg ttcgacacca aacgaagcat
     1381 tgaagtatcc cgagaggagg tgaaacactc catgatgtac gttcgagaac gcaagcgagg
     1441 cggcttcacg atcatcgagt tgctcatcgt catcgcgatc atcgccatcc tggcggcggt
     1501 ggcgattcct gcccttctgc gcgcgcagaa gtcgggcaag gagagcgcgg ccctgcaggt
     1561 cctcaagagc ctccacaacg cggagcagac cttcaaggtt cgctctaccg cgaatccgaa
     1621 gacgtttggc accctagacg agctcaaggc cgatggctcg agcccggtgg gcgaagatgg
     1681 agttgagcct gccagcggat acacgttcgc gagcgagggc gtgacgaccg atacctacac
     1741 catcattgcc acgcccccga agacggacat gattacctac acgctgaacg aaaccggtgc
     1801 ggtgacaccg tagcctggtc cgatacgtcg gtctgtcagc gggcgccggc cctgtggggc
     1861 ggcgcccgct gtgcgttgca cccctctcgc gaccgggttg tgtggcggca catcgtggca
     1921 gaatgggtat agcctctggc ggggggagcg atgcgagcgt gcccgctttt cgagcttcag
     1981 aaggcccagg gcgccgtctt tcggccccat gctggcttct cggtacccgc gcattatggt
     2041 gacccggaca ccgaatgcgc cgtcgcgcgc aagtcatgcg tcgtcaccga cctctcttac
     2101 tgcgggaagc tgagcatccg tggtgaggac cgtggcgccg tgcttgaccg cgccctcgtc
     2161 ggcgacgtga cttcactgag agcaggatgc gggctccaca ccctggcgtt gacgcccacc
     2221 gggcgcatcc tggccgacat ggtcatctac gcgctcgacg actggcacct ggtggagctt
     2281 gatctggaaa acgtgccgcg ggtggccggc cagctgttgc agctggatga cgacgcggat
     2341 gtggaggtgc aggacctgag cgaggcgtgg gggctcctct cgcttcaggg gccggagagc
     2401 gccgacgccc tggcctacct tggggtgggc attccgcccc cgagctgtat ccgcaccgcc
     2461 gtgctagcag gccatccgat ccttgtggcc gagaggagac gggcgtccgc tcctggcttc
     2521 gatctctttg ccgccgccgg agccctcatc gattgctgga actccatcct ggagcgcggc
     2581 gtccttcctg ccgggttgga cgcgctgaat gcgctgcgca ttgaggcggg gattccctgg
     2641 gcgggaacgg agctggacga gcaggtgacg ccgacggagg cgggcctgga ggcggcgctc
     2701 agcagcatca aggaaggcta tatcgggcac gccgccgtcg tgcgcgcgcg ccgtcggccc
     2761 cctgcccggc ggctgtatag acttgtgata gagacggaac tcgcccctac gcccggcgct
     2821 cctgtctatg ttgcttcgcg agtggtgggc aaggtgacaa gcgccgctcg ctgctcggct
     2881 ggtggggggg cggtggctct cgcctttctg aaacgggatt ggaacaaaga tggtgcgccg
     2941 gtgatggtgg aagtggatgg cggtaaatgc ccggcacggg tgatgcacct gccggcaatg
     3001 aaggggtgag tgtgggatgg tgatccgaga tcgcaacgcc gccgatggcg cgcgagggcg
     3061 gcggggatcc gggagtggtg cgatactgcg tgggttgatc tggccggtgc tcgtcctgtg
     3121 cagcctcggc ttcctcgcgg cgggtcgcct tcagcgtggc cgcggatggc acgagatctc
     3181 gccgggcatg gaataccggc gcctggaggt ccaagcctcg gatttgggag ataccgccga
     3241 tctccacgtg ttgcgcgtgg atctgaagca ctatcgcatc cgcctcctgc gggccagcaa
     3301 ggcgccggcg gccagcatcc gggagctggc tacgcagagc cgcgctgccg ccgcaatcaa
     3361 cgggggcttt tttgacgagg agctgcggcc tctggggctg cgcgtcaccg gtggcaagga
     3421 gctgcgccca ctccgacgcg ccaactgggg ggtcttcgtc atccgcaatg gcaagcctgc
     3481 catcctccat gcgcgcgact accggccgga gggcgtcacc gaggccctcc aatgcggacc
     3541 ccgcctcgtg gtcaacgggg aagtgacccg gctgaagaag cagtacgcgc gccgggcttg
     3601 cattggcatc gatcgggccg ggcgggtgct tctcgctgtc accaatggga gcaccatgga
     3661 cgccacctat ctggcgcgtg ttctccgcgc tcccgagccc aagagtggtc tcggctgcgt
     3721 caacgcgctc aatctcgatg gtgggggatc ggcgcagctc tacgttgacc accacggctt
     3781 tcggctggat cttccgggtg cctggggagt tccggatggc ctgggcgtct ttccccgctg
     3841 agtggccgcc ttagccagtg ggcatcacct ggctcgcccg gaaagggtga tctctctagc
     3901 tactggacta atacctgcgc gtgggaccat cggttcatat aaaggtttta taaagagccg
     3961 ataatcgtaa ttggagggag gagctactgg ccctgctcct cccaacggac ggatggccgc
     4021 ccgcgccatc cgtccgatct atccctctgc ttgccgctgc cgtaagccgt gcgggatctc
     4081 ctccgcgggc tgcatggcat cgcaaggcgc cgctcccggc accatcggtt ttcgctgggc
     4141 cggtggagag gaagcacaga gacgatggac gcctcgacct ggtctgatgg aagcagcccg
     4201 cgccgcgtga tgatcgttga tgatgaggac ctcgtgcgtg ccagccttgc cgatatggta
     4261 gagctatccg gctaccaggt gtgcggccag gccgccaatg gctatgaagc cgcccggctg
     4321 atcggcagtg cccatccgga catcgtgctc atggatatca tgatgccgga a
//
LOCUS       DAOSCV010000305         4370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12625_length_4370_cov_2.700348, whole genome shotgun sequence.
ACCESSION   DAOSCV010000305 DAOSCV010000000
VERSION     DAOSCV010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12625_length_4370_cov_2.700348"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PLY56_08600"
     CDS             <1..349
                     /locus_tag="PLY56_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ21581.1"
                     /translation="LDYLRRSEELPQVIHDIYVVASREDQRLVGVVPLREIVISPPER
                     PLGEIMETEFIRVPATESNREVARMMARYDLLALPVVDEQGILLGIVTVDDAMELLLP
                     GEWTKRFPRFFRS"
     gene            517..1755
                     /locus_tag="PLY56_08605"
     CDS             517..1755
                     /locus_tag="PLY56_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOJ21582.1"
                     /translation="MRRTGFLRVLAIIGPGLIAANAGNDAPGIATYSSAGAQYGYSLL
                     WAMLLIAFGVAIVQEMAARMGAVTGKGLTDLIRENFGVRWTLIAVIAFLIAASSTIVA
                     NFAGIGAALAFFGIPIYLSVPVIAALIWYLVVRGSYTAVERAFLAMSLVFLGYIASAF
                     LARPDWSAVASGLRPRLQTDSGYILLLVALIGTTISPHMQLFVQSAVAEKGIRMRDYP
                     LERLDVYLGSFFACTIATFIIITTGATLHRQGITIESAADAAMALRPLAGPYATALFS
                     IGLFGASMLATGVLPLATAYAVSEAFGFERGVSRSFEEAPFFTGLFTVLLVIGALVAL
                     IPGINPIDLMIYSQVIQGILLPVTLLFIMRLVNDRDLMGDYVNSRLFNFAAWSVTVVV
                     SILSVVLIVVTLLQAAGVIA"
     gene            complement(1815..2864)
                     /locus_tag="PLY56_08610"
     CDS             complement(1815..2864)
                     /locus_tag="PLY56_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOJ21583.1"
                     /translation="MPRRGERQSISVVMGHESVVPDLVRRLMTVLVLVALVTLVLWLD
                     RAGLRDTAHPERPLELPDVVYFAVVTITTVGYGDIVPVTPRARLISTFLVTPMRIVVL
                     ILFIGTAYELVFQRYREAYRMRRLKGRLANHIIVCGYGVKGHATVEELICFGHSPDEI
                     VVIDASADAARDAARLGVAALRGDATKEATLEAAVIEKATDIVVDVDRDDTTVLICLT
                     AKHLNPEICVVAAAREAENVPLIYQSGADVVVAAPVSGGRMLAIATHLTHAPRFVDDI
                     LSFGRGLGFGEYRLCQADAGRTTSEIPELSGKLVLGVYHEGRTVTYERLPGLRLREGD
                     VVIYLEAKPETAHGR"
     gene            3116..>4370
                     /locus_tag="PLY56_08615"
     CDS             3116..>4370
                     /locus_tag="PLY56_08615"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ21584.1"
                     /translation="MVDAVLRAEMMRSQPDYVVYMPKSLDGSTFDTGNEHFLVFEGPD
                     GSLMAIWTQSSYEGAGDHRILFSRSEDDGVTWAPPVRVAGPKRPGDGYMASWGFPLVS
                     KSGRIYVLWNQYQGIDDVVHQHTGTMDCCYSDDMGKTWSTPATVPMPRSPHDDPDPSV
                     PSNWIVWQLPIRDLRGRWFTGFTRWVSKAVRTPPHNDSWTAWESVVEFMRFENIDDDP
                     EPAEIRITYSAWGDQALRVPHYANPLLSIAQEPSLVRLPDDRLFCVMRTMSGYIWYSL
                     SADDGATWSNPRPLLRKDHGRPILQPLCCCPIYQLADGRYVLLHHNNDGRFEGCTPEE
                     TGKNRRPAFLALGEFRPGAEQPIWFSESKQFMDNDGARLGPLKRIDIGVYPSFTTRNG
                     NNVLWHPERKFFLLGKRVSSEFLADL"
BASE COUNT          764 a         1472 c         1319 g          815 t
ORIGIN      
        1 gttggattac cttcgccgca gtgaggagct gccccaggtg atccatgaca tctacgtcgt
       61 ggccagccgc gaggatcagc ggctcgtggg cgttgtgcct ctgcgcgaga tcgtgatctc
      121 gcccccggag cgccccctcg gcgagatcat ggagaccgag ttcatccgcg tgcccgccac
      181 ggagagcaac cgcgaggtgg cgcggatgat ggcacgctac gacctcctgg ccctcccggt
      241 tgttgatgag cagggcattc tcctggggat cgtcaccgtc gatgacgcga tggagctcct
      301 tctcccagga gagtggacca aacgcttccc tcgcttcttt cgctcgtaag gcgtccgggg
      361 ccccctggcc catgccagga gggccacccg ggaagcagct tgccacgagc cgacgcggca
      421 gcccccctgg ccgtcgtata tgtaaggaga gtagctgtcc gcgccccgtc aggcggtgcg
      481 gcagagcgac ctgtcgtgtg gttggagagg atgcgcatgc ggagaaccgg gttcctgaga
      541 gtattggcca tcatcggacc cggtctgatc gccgccaacg caggcaacga tgcccctggg
      601 atcgccacct actcctcggc gggcgctcag tatggctaca gcctcctctg ggcgatgctc
      661 ctcatcgcgt ttggcgtcgc cattgtccag gagatggccg cgcgcatggg cgccgttaca
      721 ggcaagggcc tcaccgacct gatccgcgag aacttcggcg tgcgctggac tctcatcgcg
      781 gtcatcgcct tcctcatcgc cgccagcagc accatcgtcg ccaacttcgc cggcatcggc
      841 gcggccctcg ccttcttcgg cattccgatc tatctctccg tgccggtcat cgccgccctc
      901 atctggtacc tggtcgtgcg tggcagctac acggcggtcg agcgcgcctt cctcgccatg
      961 tcgctggtct tcctgggcta catcgcgtcc gcgttcctgg cccggccgga ttggtccgcc
     1021 gttgccagcg ggctgcggcc ccggctccag acggacagcg gttacatcct gttgctcgtc
     1081 gcgctcatcg gtacgacgat ctcgccgcat atgcagctct tcgtgcagtc cgccgtcgcc
     1141 gagaagggca tccgcatgcg cgactacccc ctggagcgcc tcgatgtcta tctcgggtcg
     1201 ttctttgcct gcacgatcgc gaccttcatc atcatcacga ctggcgcgac gctccaccga
     1261 caggggatca cgattgaaag cgccgccgac gcggcgatgg cgttgcgccc gctcgccggc
     1321 ccctacgcga ccgcgctctt ttccattggc ctcttcggcg cgtcgatgct tgccacagga
     1381 gtgttacccc tggcgaccgc gtatgccgtc agcgaggcgt tcggcttcga gcgaggcgtc
     1441 tcgcgctcct tcgaagaggc gccctttttc accgggctct tcacggtgct gcttgtgatc
     1501 ggtgccctgg tagcgctgat ccctggcatc aacccgatcg atctcatgat ctactctcag
     1561 gtgatccagg gcatcctcct ccccgtcaca ctcctcttta tcatgcgcct ggtgaatgac
     1621 cgcgatctga tgggcgacta tgtgaacagc cgcctgttca acttcgccgc atggagcgtg
     1681 acagtcgtgg tcagcatcct ctcggtggtg ctcatcgtgg tgacgctgct gcaagccgca
     1741 ggcgtgatcg cctgagccgc gggatgctct tgcgctcgct tgcgatggag ccggatcgat
     1801 gcgcggcgaa gacactacct tccgtgagcc gtctctggtt tggcctcaag gtagatcacc
     1861 acatcaccct ctcggagacg caaccccggg aggcgctcgt aagtaaccgt tctcccctcg
     1921 tggtatacgc ccagcacgag tttcccggat agctccggaa tctcgctcgt cgtgcgcccg
     1981 gcatcggcct ggcagagccg gtactcccca aaacccaggc cccggccaaa agagaggata
     2041 tcatcaacga aacgcggcgc atgggtgagg tgagtggcga tcgcgagcat gcgcccgccc
     2101 gatacgggag cggccaccac cacatcggca ccgctctgat agatgagggg aacgttctct
     2161 gcctcccgcg cggcggccac cacgcagatc tcgggattga ggtgcttcgc cgtcaggcag
     2221 atgagcacgg tggtgtcgtc tcgatccaca tccacgacga tgtcggtcgc cttctcaatc
     2281 accgccgcct cgagcgtcgc ttccttggtc gcatcgccgc gcagcgccgc cacccccagg
     2341 cgcgccgcgt ctcgggccgc atcggcgctg gcatcgatca ccacgatctc gtccggcgag
     2401 tgtccaaagc agatcaactc ctcgacggtg gcatggcctt tcacgccata gccgcaaacg
     2461 ataatgtgat tggccagccg tcccttgagt cgacgcatcc tgtatgcctc tcgatagcgc
     2521 tgaaacacca gttcatacgc cgtgccaatg aacaggatca agaccacgat ccgcatcggc
     2581 gtcaccagaa acgtgctgat cagccgcgcg cgcggcgtca cggggacaat atcgccgtag
     2641 cccaccgtgg tgatcgtgac aaccgcgaaa tagaccacgt ccggcagctc taacgggcgc
     2701 tcggggtggg cggtatcacg cagccccgcg cggtccagcc acaacaccag ggtcaccagg
     2761 gcaacgagca cgaggaccgt catcaaccgg cgaacgagat ccggaacgac actctcgtgc
     2821 cccatcacga cactgatgct ctgtctttct cctcgtcgcg gcatcgcctt cactactccc
     2881 cctcgttgcc tacccacttt ttgaccgtgg cacacgcttt cagcaccaag cggtcacatc
     2941 atctagacgg ctagacacct cgggccaggc catgtaacgc cttcagaacc acgagaggac
     3001 cccagcgtgg ctctgctggg ggagtccaaa ggggtccgag tctccagagg ccggccagca
     3061 ggaggtctcc ccggatagga ggaacccctc ggtgtgaagg gacctggagg aagagatggt
     3121 ggatgcagta ctacgcgccg agatgatgag gagccagcca gactacgtgg tgtacatgcc
     3181 gaaaagcctg gatgggtcca ccttcgatac ggggaacgaa cacttcctgg tctttgaggg
     3241 gccagacggc tctctgatgg caatctggac tcaaagcagc tatgagggcg cgggcgatca
     3301 ccgcattctg ttttcccgca gcgaggatga cggcgtcacg tgggcgccgc cggtccgggt
     3361 ggctggcccc aagcgccctg gcgatggcta catggccagc tggggcttcc cgctggtctc
     3421 caaatccggg cgcatctatg tcctttggaa ccagtaccag ggaatcgatg acgtggtgca
     3481 tcagcacacc ggcaccatgg attgctgcta cagcgatgat atgggaaaga cgtggtcgac
     3541 acccgccacc gtgccgatgc cgcgcagccc gcatgatgac ccggacccga gcgtaccttc
     3601 caattggatc gtctggcagc tgccgatccg cgatctgcga gggaggtggt tcaccgggtt
     3661 cacgcgctgg gtgagcaagg cggtgcgtac ccctccgcac aacgatagct ggacggcctg
     3721 ggaaagcgtc gtcgagttca tgcgctttga aaacatcgat gacgacccag agccggcgga
     3781 gatccgcatc acctactcgg cctggggcga ccaggcgttg cgagtgcccc actacgccaa
     3841 tccgctgttg agcatcgctc aggagcccag tctggtgcgc ctgccggatg accgtctctt
     3901 ctgcgtgatg cgcacgatgt ccgggtacat ctggtacagc ctctcggcag acgatggcgc
     3961 cacctggagc aatccccgcc cgctgctgcg caaggatcat ggccggccga tcctgcaacc
     4021 cctctgctgc tgcccgatct accagctcgc ggatggccgc tatgtgctct tgcaccacaa
     4081 caacgacggc cgcttcgaag ggtgcacacc ggaggagacg ggcaagaacc gccgcccggc
     4141 gttcctcgcc ctgggtgagt ttcgccccgg agccgaacag ccgatatggt tcagcgagtc
     4201 gaaacagttc atggacaacg acggcgcgcg cctcggcccg ctgaagcgga tcgatatcgg
     4261 cgtctacccg agcttcacca cgcgaaacgg gaacaacgtc ctctggcatc ccgagcggaa
     4321 gttcttcctc ctcggcaagc gcgtctcctc cgagtttctg gcagacctgg
//
LOCUS       DAOSCV010000306         4350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12698_length_4350_cov_2.324098, whole genome shotgun sequence.
ACCESSION   DAOSCV010000306 DAOSCV010000000
VERSION     DAOSCV010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_4350_cov_2.324098"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>358)
                     /locus_tag="PLY56_08620"
     CDS             complement(<1..>358)
                     /locus_tag="PLY56_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012847914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOJ21585.1"
                     /translation="ALASTLLSMVREVVFGVGFALLLPLFFGLDGVLYSMPAADVFTF
                     VISAAVIRRTLRSLADMDAILAQITEGKVERALVIGGGFIGLELTEALRERGIGVTLV
                     ELAPQVMAPLDPEMAAP"
     assembly_gap    359..368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<369..732)
                     /locus_tag="PLY56_08625"
     CDS             complement(<369..732)
                     /locus_tag="PLY56_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005446897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ21586.1"
                     /translation="MPRSTRIVVIGGVAGGASAATRARRISEDAEIILLERGPHISYA
                     NCGLPYHIGGVIAEREKLLVATPEQLSTRYRIDVRTRTEAVAIDRERKEVVARNLVTG
                     VEERIPYDKLILSPGAEPV"
     gene            1139..2218
                     /locus_tag="PLY56_08630"
     CDS             1139..2218
                     /locus_tag="PLY56_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ21587.1"
                     /translation="MKLIDRYVVRELGVPFGIGISVAVMLLIGTLLFNNASEFISRGI
                     PLLLVIKLVLYRAPQFSVIAMPMAMGFAASLAINRLARDSEITPMRMAGIPFRRIVFP
                     LALVGAVTSLGSYLLAEKVAPWANEKGNQIVRQIWMQSVVPVLQPNVVFRTEGYTFYV
                     GWLNKASKDLFHLREVLVYDTRRPGYPVWYVAPEATTTRRFWTLRRVVRRDIGKDGLS
                     TVETYLPELTIDLKKDMDFIGGMATPEEMTGDQLMAQIRMLKETGAQNLARSYAVQYH
                     SRFALPLACLILAVLSAPLSLRFSRGGTFVGILVSIIVGFLYWNIMLLTKILGENGVL
                     PPLVAAWSMNALFGVVAAILVWRTG"
     gene            4165..4350
                     /locus_tag="PLY56_08635"
                     /pseudo
     CDS             4165..4350
                     /locus_tag="PLY56_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
BASE COUNT          724 a         1494 c         1326 g          796 t
ORIGIN      
        1 ggggcgcggc catctcgggg tcgagcgggg ccatcacctg gggcgccagc tccaccagtg
       61 tcacgccgat tccgcgctcg cgcagggctt cggtgagctc caggccgatg aacccgccgc
      121 cgatcaccag cgcgcgctcc accttgccct cggttatctg ggcgaggatt gcgtccatat
      181 cggccagcga gcggagcgtg cggcggatca cagctgctga aatcacaaaa gtgaaaacat
      241 ccgccgccgg catggaatac agcacgccgt cgagcccgaa gaacagcggg agcagcagcg
      301 cgaagcccac gccgaacacc acctcccgca ccatggacag cagcgtggag gccagcgcnn
      361 nnnnnnnngc accggctcgg cgccggggct caggatcagc ttgtcataag ggatgcgctc
      421 ctcgacgccg gtcaccaggt tgcgcgcgac cacctccttg cgctcgcggt cgatagcaac
      481 ggcttccgtg cgagtgcgca cgtcgatgcg gtagcgcgtg ctcaactgct ctggggtcgc
      541 caccaacagc ttctcgcgct cggcaatcac cccgccgata tgatagggca gaccgcagtt
      601 agcgtaggag atgtgcgggc cccgctccaa caagatgatc tccgcatcct ctgagatgcg
      661 tcgcgcccgc gtcgcggcgg acgctccgcc tgccactcca ccaatcacga caatccgggt
      721 acttcgaggc attcgcgtgc tccttctcca caaggaaacc tctcatgaga gggtgccgca
      781 cctcgcccgc ggccctcttt tgattagata caggccacgc tcggaggatt cgcctccgtt
      841 ttcggagtct tcctgccgcg cccgccacga cgataccgcg gataaggcga cggatgcgga
      901 tcgggaagcc tgcggccggc ccaataggcg cgggcccgaa gacccgggct gcttccccgc
      961 agcggccctg ccggccttcc ccgacagcct gctttgcttt cgcatgtggg ccggcccagc
     1021 ccggcagggt ggccattgcc acccgggtcc aacctccctc tgaatgtggc ctctcttgtg
     1081 gccccgccgg gtcgctggcg tgcttgccgc ccctccccaa agatgctata atggcgccgt
     1141 gaagctcatc gaccgctatg tggtacgcga gttaggggtg ccgttcggca tcgggatctc
     1201 cgttgccgtg atgctcctga tcggcacatt gcttttcaac aatgccagtg agttcatttc
     1261 gcgtggcatc ccgctgctgt tggtgatcaa actggtcctc tatcgcgcgc cacagttctc
     1321 ggtgatcgcc atgccaatgg cgatgggctt tgctgcttcg cttgcgatca accgtctggc
     1381 ccgcgatagc gagatcaccc cgatgcggat ggcggggatc cctttccggc gaatcgtgtt
     1441 tcctctagcc cttgtcggcg ccgtcaccag cctgggctcc tacctccttg cagagaaggt
     1501 ggcaccctgg gcgaacgaaa aggggaatca gatcgtccgt cagatatgga tgcagtcggt
     1561 agtgccggtg ttgcagccaa acgtcgtttt cagaaccgag ggctacacct tctacgtggg
     1621 ctggctcaat aaggcgagca aggatctctt tcacctgcgt gaagtgctgg tctacgatac
     1681 ccgtcgcccg ggctatccgg tgtggtatgt ggccccggag gcgaccacga cgcgccgttt
     1741 ctggaccctg cggcgtgttg tccgccgcga cattggcaag gatggcctct cgaccgtcga
     1801 gacctacctt cccgagttga cgatcgacct gaagaaggat atggacttca tcgggggcat
     1861 ggcaaccccc gaggagatga ccggcgacca gttgatggcg cagatccgca tgctgaagga
     1921 gacaggcgct cagaacctgg cgcgctccta cgcggtgcag taccactcgc gctttgctct
     1981 gccgctggcg tgcttgatcc tggcggtact cagcgccccg ctcagcctgc gcttctcgcg
     2041 cggcggcacc tttgtcggca tccttgtcag catcattgta gggttcctct actggaatat
     2101 catgctcctc accaagatct tgggtgaaaa cggggtgctc cccccactcg tcgcggcgtg
     2161 gtcgatgaac gccctctttg gtgttgtcgc cgcgattctg gtgtggcgca ccgggtgagc
     2221 gatcacggcg tctatcggga atcggcagaa gtaccccgca tgagaaacgt tgtcggaggg
     2281 ttggcggctt catcgcccgg cgccaggcta ttgccgtgcc agggcgcggc gcgcgcacag
     2341 cgcgaggttt tggcgcaccg cgacgtgggc gcgagggccg ctctcctgcg ggcaattctc
     2401 ctggccgcct gcctcctcgg cctccccttc accgcagttt cgggcgatac ggccgcccag
     2461 catgcacccg tcgcccagac ggaggtcgcc ccctcagcca cggatgacac ggccactcag
     2521 ggagcgccgg gcgccgagac ggaggccgcc cccccagccg caggtgacac ggctacccag
     2581 aatgcacccg ccgccgcgac ggaggccgcg cctgcgcccc aggccaccct ggaggctgcc
     2641 gggcagccgg tggcttcccc gccggcccct gccggcaagg agatcccctt ccgcatcctc
     2701 gaggccgacg ctatcagcta tgatgagcag tcgaaccgcc tggttgctca ggggcatgtg
     2761 gtggtggagt acaaggatta ccgggtctcc gcggatgttt tggccgccga tttcgaggat
     2821 cgggtggcca cctttcgcgg gcgcgtggtc ctcaccacgc ccgagcgggt gatccacggc
     2881 acgctcctct cgctcgatct ccgctcgcgc gcctacgcgc tcgagggcgc gaggatgctg
     2941 ctggctcctg aggaggtgca gatgggcgcg caacagcccc tctacgcctc aagcgcacgc
     3001 atcgacgggg agcccggagt ggtctccgtg aaacagggcg ggatcacctc ttgcgacctt
     3061 ggctcgcctc actacctctt cgatgcccag aagatccagg tgattcccgg caagcggctc
     3121 acggcgcgca aggtttcggt ctacgccctt gggcacaagc tgatcaccct ccccgggctg
     3181 acgattcccc tctcctcgtt cgatacgccg cggagcaacc ggtttgtgcc cctcttcggt
     3241 cagagcctgg acgaaggcta cttcatgaaa gccgcctatt tctacgttct cggctcgctc
     3301 atcgggaatc tgcgcctaga cctgatgtcg aagaagggca ccgggatcgg cctcgatcaa
     3361 ccttatgatt tcccgaacta ctccggcggg atccacgtgt accagctcaa caaccggcgt
     3421 gacaaccgca gggactttac ggcgcgcgtt gatcaccagc accaactcgg cgagtttgcc
     3481 ctccgctttg gcggcgacta ccgcttgaac tcgctcccct atgccacggc ggacagctcc
     3541 agcctgctga cagacctggc gatcacccgg cgcggcgccg atggaaccac caccgacctc
     3601 ggcttcaacc gcagtgccag cacctatagc gggaccacca ccggcaactg gctcgcgcgc
     3661 ctccgtcact cgcagaactt tggctttgtg cgcgccaacc tcagcatgga catgagccag
     3721 ccgttcggga tgagcacggc ttcctccggg cgcctgagtt ccaggttgga cctccaacac
     3781 tcgaatcggc ttttcgatgc cggcctcctg atcgatcgca tcgacaccct gggcggcacc
     3841 gcgaacttct tcccggggat gcagcacctc ccggagttgc gcctggccac ggcgagccag
     3901 cgcctgacca actccgactt cttcacaaag acgctgccga tctcgatgga gctacgcgtc
     3961 ggcaacctgc gcgagggcgg ctatgcgctc tctggctctt cgagtgcgcc cgcgatgacc
     4021 gagcggttgc gcacctattt ccgcgtcagc agcagcccgc aggtgttgcc atccaagggc
     4081 cggtttgccg tcacgctccc ggtcgatttc cagcagaatt actaccgaga ggatgaagcg
     4141 atgtgggcgg cgggtatcag cccggcgctc acctaccgca tgggtgcgca caccaccagc
     4201 gacgacccca ccaagtatcg ggatgacgcc gaactcgact tctgggtcag gcacgacccg
     4261 atcgtgcgct tcgaggccta cctccgctct cgcggggccg acgacgcgtt cttcgcggaa
     4321 ctcgcggagg aggcgcacga cgcgctctac
//
LOCUS       DAOSCV010000307         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12704_length_4349_cov_2.746856, whole genome shotgun sequence.
ACCESSION   DAOSCV010000307 DAOSCV010000000
VERSION     DAOSCV010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12704_length_4349_cov_2.746856"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..404
                     /locus_tag="PLY56_08640"
     CDS             99..404
                     /locus_tag="PLY56_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21588.1"
                     /translation="MRGGHRESALVAEIVRALRKLPDVIVRKRHGSAWGVAGDPDLYG
                     SINGRHFEIEVKRPGAEGPTPLQQARLRQWAATGALVGVARSVEDALRIVSANGRHG"
     gene            401..631
                     /locus_tag="PLY56_08645"
     CDS             401..631
                     /locus_tag="PLY56_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21589.1"
                     /translation="MRVAPYSLEALREYRLKQAGKPAPTPVRLADIPPDAPMEEGIVI
                     GCWNGRAFVSWEKWLASLPAEALRVSAEDDHG"
     gene            624..2396
                     /locus_tag="PLY56_08650"
     CDS             624..2396
                     /locus_tag="PLY56_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21590.1"
                     /translation="MAEPGTPRWFLEALFADMPGELYLLIWTLADKRSRWFRDLDAAA
                     AAVEALASQDVYVGVGLSTKDYGPTQRCPSEEIVGVVAAWADFDILSEAHPKKSLPST
                     LEQALSVVPPAMPPSIVISTGNGAHAWWILREPYLFDSDEDRRWAASVVLRWQTLLRY
                     NAANRGWAFDRLADLARVLRIPGTLNCKDPAHPKSVTVYQVTDRRYNLSDLEEYLDEL
                     GIEDPEAHEQKRRAWAERFKDKPIIVNLKAELPEDLVRRLVDADLRFRNTWFRQRHDL
                     KDQSQSGYDLALADFGMDAGLSEQQIVDLIIQHRRMHRQKARTRADYYERTLAKAAQR
                     SDAVRELPPLCQATADASATDRPAEQGVERTEPPEAKQERPSDAMAAKAALCEYISQI
                     LGVRILRIVKITGKEPTYQLILENAKIELAHVGKLLDQNSVRMAIATATNKLIKRLKP
                     KLWDQLAQSMLDALIEEEGGPETQLEGAMRMYLEQYLTDVAFIPAIEGQSSNAIRRPM
                     VLGGQIAVNSSDLQLFINKTFLQNLSVKAVASMLAAVGAKSIRVRSAKNGEQGRWLLP
                     ADQFNPADYTAPAKPEEAGDAKAK"
     gene            2380..4086
                     /locus_tag="PLY56_08655"
     CDS             2380..4086
                     /locus_tag="PLY56_08655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOJ21591.1"
                     /translation="MPRPSEIQSAPVGTLEGIGEQDHSVTAEYRIFGPPGTGKTTNLS
                     RQIRRAVERFGPDSVLVTSFSKAAAAELAGRDLPISTDRIGTLHSHCYHALGAPAIAE
                     ANVEEWNRENPHLAISPVKKQTRLDGEEGIEDDEEQRDGDRWLRDLNRFRGMMIPPEW
                     WPANVRHFAARWEQYKAAHDLLDFCDLIETCYRDLPMAPTRPEVIFVDEAQDLSPLQM
                     ALIRKWGERAQYFVVVGDEDQLLYSWCGCVPESLLDPPLSPDRIIILKQSYRVPRTVH
                     AAADRLIRQVTRRQEKPYLPRPAEGLCTFTGGTWRSPGYGILKAAIEHLERGQTVMFL
                     ASCSYMLRPVIQVLRKNGIPFHNPHRKANGLWNPLRVGRRGSAASRILALLVAHPDLG
                     QDSRPWTRRDLALWAEWLSSRGILKRGTKGEIEGANAADAVTIETLDQVFEPGALEEL
                     LSCFEGDYRRLLEWWRRRLTASVASRVEFPANIAMARGPRALLETPRVTVGTIHSVKG
                     GEADVVFLFPDLSQAGDAAYQRFGSPRDAVIRTFYVGMTRAREVLYIAEKASAMAASL
                     GA"
     gene            4160..>4349
                     /locus_tag="PLY56_08660"
     CDS             4160..>4349
                     /locus_tag="PLY56_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21592.1"
                     /translation="MIPRVQHPKVHEAVKCRRDGWWWFFRYWADEVRPDGTAQTVRKR
                     QWLGPSRGEKKISKKEAEV"
BASE COUNT          827 a         1378 c         1443 g          701 t
ORIGIN      
        1 cgcatcggcg gcaagctcgg ggtggccagg ggtgagtcga agccacgcgc catcacccta
       61 gccatcgccc gcgccctggg gctggaggag gtggacgaat gaggggtggg caccgtgaat
      121 ccgcgctggt cgccgagatc gtgcgcgctc tccggaagct gccggacgtg atcgtgcgaa
      181 agcggcacgg gagcgcctgg ggtgtcgccg gagatccgga cttgtacggc tccatcaacg
      241 gccgtcactt cgaaatcgag gtcaagcggc ctggcgcgga gggcccaacg ccactccagc
      301 aggcccggct ccgacagtgg gcggccaccg gcgccctggt cggcgtagcc cgcagcgttg
      361 aggatgcgct gcgcatcgtg agcgcgaacg gaaggcacgg atgagggtcg caccgtacag
      421 cctggaagcg ctgcgcgaat accgcctgaa gcaggccggc aagccggcgc cgactccagt
      481 acggctcgcg gacatcccgc ccgacgctcc catggaagaa ggcatcgtga tcggctgctg
      541 gaacggcagg gccttcgtga gctgggagaa gtggctggct tcgctgccag ccgaggcgct
      601 gagggtgtcg gcggaggacg atcatggctg agccgggcac gccgcgctgg tttctcgagg
      661 cgctgttcgc cgacatgccc ggcgagctct acctgcttat ctggacgctg gccgacaagc
      721 gctcccgctg gttccgcgac cttgatgccg cggctgccgc ggtagaagcc ctggccagcc
      781 aggatgtcta cgtcggcgtg ggcctgtcca cgaaggacta cggccccacc cagcgctgcc
      841 cgtcggagga aatcgtaggc gtggtggctg cctgggcgga ctttgacatc ctctccgagg
      901 cgcatccaaa gaaatccctg ccgtccacgc tcgaacaggc tctttcggtc gtgccgccgg
      961 cgatgccgcc aagcatcgtg atatcgaccg gcaacggcgc ccacgcctgg tggattctgc
     1021 gagagcccta cctgttcgac agcgatgagg atcggcgttg ggcggcctcc gtagtgctcc
     1081 gctggcagac gctactcagg tacaacgccg ccaaccgcgg gtgggccttc gaccggttgg
     1141 cagacctcgc ccgagtgctc cgcatccccg gaacgttgaa ctgcaaggac cccgcgcacc
     1201 cgaaatcggt gaccgtctac caggtcacgg accgccgcta caacctctca gacctcgagg
     1261 aatacctgga tgagcttggc atcgaagacc cggaagccca tgagcagaag cgccgcgcgt
     1321 gggccgagcg gttcaaagac aagcccatca tcgtgaactt gaaagccgag ctgccggaag
     1381 acctcgtcag acggttggtt gatgccgacc tgcgcttccg gaatacctgg ttccgccaga
     1441 ggcacgacct gaaagaccag agccagagcg ggtacgacct cgcactggcg gatttcggca
     1501 tggatgctgg cttgagcgag cagcagatcg tcgatttgat catccaacac cggcgcatgc
     1561 accgacagaa ggcgcggacg cgcgcggact actacgagcg cacgctcgcc aaggcggcgc
     1621 agcgcagtga cgccgtaaga gaactgcccc cactgtgcca ggcgaccgcc gatgcctcgg
     1681 ccaccgatag acctgccgaa caaggcgtgg agcggacgga gccgccggaa gcgaagcaag
     1741 agcgccccag tgatgcgatg gccgccaagg cggcgctctg cgaatacatc tcccagatcc
     1801 tcggcgtccg cattctgcga atagtgaaga tcaccggcaa ggagccgacg tatcagctca
     1861 ttctggagaa cgccaagatc gagcttgcac acgtcggcaa gctcctcgac cagaacagcg
     1921 tccgcatggc gattgcgacg gcaacgaaca aactgatcaa gcgcctcaag ccgaaactct
     1981 gggatcaact tgcccagtcg atgctggacg cactgatcga agaggagggc ggaccggaaa
     2041 cgcaacttga gggtgccatg cggatgtatc tggagcaata cctcacagac gtcgcattca
     2101 tcccggcgat cgaagggcag tcctcgaacg ccattcgccg ccccatggtg ctcggtgggc
     2161 agatcgccgt gaattcctcc gacctccagc tcttcatcaa caagaccttc ctccagaacc
     2221 tctccgtcaa agcggtggcc tcgatgctgg cggccgtcgg ggccaagagc atccgggtgc
     2281 gcagcgccaa gaacggcgag cagggacgct ggctgctgcc tgccgatcaa tttaatccag
     2341 ccgactacac cgctccagcc aaacccgagg aggccggcga tgccaaggcc aagtgagatt
     2401 cagagtgcgc ccgtcgggac gctcgagggt atcggcgagc aggatcactc ggtcacagca
     2461 gagtatcgaa tcttcggccc gccggggaca ggtaagacga ccaatctctc gcgccagatt
     2521 cggcgtgccg tggagcgatt cgggccagat tctgtgctgg ttacgagctt ctcgaaggcc
     2581 gctgctgcgg agctggccgg tcgcgaccta ccaatcagca cagatcgcat tgggaccctg
     2641 cactcgcact gctaccacgc cctcggcgcg cccgcgattg ctgaggccaa cgtcgaggag
     2701 tggaaccggg agaatccgca tctggcgatc tcgccggtca aaaagcaaac caggctggac
     2761 ggcgaagaag gaatcgaaga cgacgaggag caaagggacg gagatcgctg gcttcgggat
     2821 ttgaatcgct ttcgcgggat gatgattccg ccggagtggt ggccggcgaa cgtgcgccac
     2881 ttcgcagctc gctgggagca gtacaaagcg gcccacgacc tactggattt ctgtgacttg
     2941 atcgagacct gctaccgcga cctgccaatg gcgcccacac gaccggaggt catcttcgtc
     3001 gacgaagccc aagatctttc cccactccag atggctctca tccgcaagtg gggcgagcgg
     3061 gcgcagtact tcgtggtcgt tggggacgag gaccagcttc tgtactcctg gtgcggctgc
     3121 gtgcctgagt cgcttctgga tccgccgctc tcgcctgatc gcattatcat tctcaagcaa
     3181 agctaccgcg tgccccgcac cgtgcacgcc gccgccgaca ggctcatccg ccaggttacg
     3241 cgccggcagg agaagcccta cctgccacgg ccggcggagg ggctatgcac ctttacgggc
     3301 ggtacctggc gctcgcccgg gtacggaatt cttaaggcgg cgatcgagca cctggagcgc
     3361 ggccagacgg tgatgttcct ggcaagctgc tcctacatgc tgcgccctgt gatccaggtg
     3421 ctccgcaaga acgggatccc gtttcataac ccgcatcgga aggccaacgg gctctggaat
     3481 ccgctgcgcg tcggccggcg cggctcggct gccagccgaa tcctcgctct gcttgtggcg
     3541 cacccggatt tgggccagga ctcgaggccc tggacccgcc gagatctggc actgtgggca
     3601 gagtggctgt cctcgagagg catcctgaag cggggcacca aaggcgagat cgagggagcg
     3661 aatgcggccg atgcggtgac gatcgagacg cttgatcagg tgtttgagcc cggtgcgctc
     3721 gaggagctct tgagctgctt cgagggcgac taccgaaggc tgcttgagtg gtggcgccgg
     3781 cgcctgaccg cctcagtggc cagccgcgtt gagttcccgg ccaatatcgc catggcgcgg
     3841 ggcccgcggg cgctgctaga gacgccgcgc gtgaccgtcg gcacgatcca ctcggtgaaa
     3901 ggcggcgagg ccgacgtcgt gttcctgttc ccggatctca gccaggcggg cgatgcggcc
     3961 tatcagcggt tcggctcgcc tcgggacgcc gtgatccgga cgttctacgt gggcatgacc
     4021 cgggcgcgtg aggtcctgta catcgccgag aaggcctcgg ccatggctgc ctcgctaggg
     4081 gcctaactta ttcagaacat tggacttgat tcccatggga gccgaaggta accttctgac
     4141 gctgaccgga ggcttgcgag tgatacccag ggttcagcac ccgaaggtcc acgaggcggt
     4201 gaagtgccgc cgggacgggt ggtggtggtt cttccgctac tgggccgatg aagttcgccc
     4261 ggacgggacc gcgcagacgg tgcgcaagcg ccagtggctt ggccccagcc ggggcgagaa
     4321 gaagatctcg aagaaggaag ccgaggtcg
//
LOCUS       DAOSCV010000308         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12711_length_4347_cov_3.063141, whole genome shotgun sequence.
ACCESSION   DAOSCV010000308 DAOSCV010000000
VERSION     DAOSCV010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12711_length_4347_cov_3.063141"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..328
                     /locus_tag="PLY56_08665"
     CDS             179..328
                     /locus_tag="PLY56_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21593.1"
                     /translation="MVSAWATESGLALGQIKVDGKSNGITALPGLLKLLELANCVVTA
                     PSVRR"
     gene            295..621
                     /locus_tag="PLY56_08670"
     CDS             295..621
                     /locus_tag="PLY56_08670"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HOJ21594.1"
                     /translation="MRGDRTIGEKVTSEMRHFISSLEGGAAKLIRPIRSHWCIENSLH
                     WGLDVSMGEDACRIWQDHAPANMATLRRVALTLPKQERTNKRGIKARSKKAGLDGNYL
                     LKVLVG"
     gene            complement(758..2287)
                     /locus_tag="PLY56_08675"
     CDS             complement(758..2287)
                     /locus_tag="PLY56_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOJ21595.1"
                     /translation="MGKPTGFLEIPRMKAPRRPVEERVRDWREVEQPLPTDALQKQAA
                     RCMDCGIPFCHQGCPLGNVMPDFNDLVYRNRWDEAIERLHATNNFPEFTGRLCPAPCE
                     ASCVLGATADPVTIKYIEHAIIERAWQEGWVRPQPPAIETGKRVAIIGSGPAGLACAQ
                     QLRRAGHQVTLFERADRIGGLLRYGIPQFKLEKHVLDRRLAQLEAEGVEFRPGVNVGV
                     ECDAAALRREYDAIVLAGGATHARDLNIPGRQLKGIYQAMHFLPMANRFAIGDVVNEA
                     GDILVKPVPPYEGDGQPVALLSARGRKVVIIGGGDTGADCLGTALRQQCESVAQFEIV
                     PKPPETRAPQNPWPQWSNIFRVSSAHEEGGIREYQISTQEFLGDAEGNVCALRTVRVE
                     MQMQAGRPTFVEIPGTEEIWEADLVLLAMGFLGPEKGAVMEQLGVEFDERGNVKSDEY
                     RQTNVEGVFTAGDMARGQSLIVWAIAEGRHCAHGVDRYLMGESALPRPLAHGQDKRPF
                     T"
     gene            complement(2342..4347)
                     /locus_tag="PLY56_08680"
                     /pseudo
     CDS             complement(2342..4347)
                     /locus_tag="PLY56_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019503722.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
BASE COUNT          686 a         1465 c         1454 g          742 t
ORIGIN      
        1 agggcttcgc tcgcaagcag cgcggtgatc tatcgccgcg cgccgggttg cgcgctacct
       61 ccgaggcttc ggattcctta tatcggaatc tcagggtgct ctttggctgg tccgggcttt
      121 ggacaggaag acactgcgcc atagctttga tatagccaca ggctaggctg ccatccatat
      181 ggtaagcgcc tgggcgacgg aaagcgggct tgccttgggg cagatcaagg tggacggcaa
      241 gagcaacggg atcacggcgt tgcccgggct gctcaagctg ctggaactgg cgaattgcgt
      301 ggtgaccgca ccatcggtga gaaggtgacc agcgagatgc gccacttcat cagcagcctt
      361 gagggaggcg cggcgaagtt gatacggccc attcggtcgc actggtgcat cgagaacagc
      421 ttgcactggg ggctggatgt aagcatggga gaggatgcct gccgaatctg gcaggatcat
      481 gctccggcga acatggccac gctacggcgg gttgctctca ccctacccaa gcaggagcgc
      541 accaataaac ggggtatcaa ggcccgtagc aagaaggccg gcctggatgg caactacctg
      601 ctgaaggtgt tggtcggata gccatcaata agatgctctc gccctgcgag cgcagtctcg
      661 gcttcgcggc agggacggcg cgaggcgcag gccgcaccgg aaaggcgccc gcataccggg
      721 gcaagagccc ggtaggatcc cccagccgtg gccccggcta agtaaatggc cgcttgtcct
      781 ggccgtgggc cagggggcgc gggagggcgc tctcgcccat caggtagcgg tcgaccccgt
      841 gggcgcagtg gcggccttcg gcgatggccc agacgatcag gctttgcccg cgcgccatgt
      901 cgccggcggt gaagacgcct tctacgttgg tctgccggta ctcgtccgac ttcacgttgc
      961 cgcgctcgtc gaactcgacg ccgagctgct ccatcacggc gcccttctca gggccgagga
     1021 agcccatcgc cagcagcacc aggtcggctt cccagatctc ctcggtacca gggatctcca
     1081 cgaacgtcgg ccggccggcc tgcatctgca tctcgacgcg cactgtgcgt agggcgcaga
     1141 cgttcccctc tgcgtcgccc aggaactcct gggtgctgat ctggtactcg cggatgccgc
     1201 cttcctcgtg cgccgaggag acgcggaaga tgttgctcca ctgcggccag gggttctgcg
     1261 gcgcgcgcgt ctccggcggc ttcggcacga tctcgaactg ggcgacgctc tcgcactgct
     1321 ggcgcaacgc cgtgccgagg cagtcggccc cggtgtcgcc gccgccgatg atgaccacct
     1381 tgcggcctct ggcggagagg agagcgaccg gctggccgtc gccttcatag gggggcaccg
     1441 gcttcaccag aatgtcgccc gcttcattga caacatcgcc aatggcaaag cggttggcca
     1501 tcgggaggaa gtgcatcgcc tggtagatgc ccttgagctg acggccgggg atgttcaggt
     1561 cgcgtgcgtg cgtcgctccg ccggcgagga cgatggcatc gtactctcgg cgtagcgcgg
     1621 cggcgtcgca ctccacgccc acgttcacgc cgggccggaa ctcgacgccc tcggcctcca
     1681 gttgcgccaa ccggcggtcc agaacgtgct tctccagctt gaactggggg atgccatagc
     1741 gcaacaggcc gccgatgcgg tcggcgcgct cgaagagtgt cacctggtgg ccggcgcggc
     1801 ggagctgctg ggcgcacgcc aggccggcag ggccggagcc gatgatggca acgcgcttgc
     1861 ccgtctcgat ggctggcggc tggggccgaa cccagccctc ttgccaggca cgttcgatga
     1921 tggcatgctc gatgtacttg atggtcaccg ggtcggcggt agcgccgagg acgcacgagg
     1981 cctcgcacgg cgccgggcag agccggccgg tgaactccgg gaagttgttg gtagcgtgga
     2041 gacgctcgat cgcctcatcc caccggttcc ggtagaccag gtcgttgaag tccggcatca
     2101 cgttgccgag ggggcacccc tggtggcaga aggggatgcc gcagtccatg cagcgtgccg
     2161 cctgcttctg cagcgcgtcc gtggggagcg gctgctcgac ctcgcgccaa tcccgcacgc
     2221 gctcctcgac agggcggcgc ggcgccttca ttcgcggtat ctcaaggaat ccggttggct
     2281 tgcccatctg tctgctctcc tcgatttcct gccacggtcg ggcatgatat accctgccgc
     2341 ttcaggcgac aacgggagag gccggactga cagccgacgc gcccgccagc gcttcgcttg
     2401 cctgctcccg cttccgctct tcgatcacct tgcggtagtc gcgagggtag accttgacga
     2461 accggccctg cagcgcctcc cactgcgcca ggacgcgggc agccacgggg ctgccggtct
     2521 cccgtgcgtg ctcctcgacg agcgcacgta ccatcgcctg atcttccagg tctgtgaggg
     2581 gctcaatgtc caccatctcc tggttgcagc gcagggcaaa gtcgcccgcc tcatcgagga
     2641 cgaaggcgag gccgccgctc atgcccgccg cgaagttgcg ccccgtgggg ccgaggatga
     2701 cggcgacgcc acccgtcatg tactcgcagc cgtggtcgcc gcacccctcg acgacggcgt
     2761 aggcacccga gttgcgcacg cagaagcgct cgcccgccag gccctggaag aaggcacggc
     2821 ctcgcgtcgc cccgtagaga gccgcgttgc ccacgagaac ctgctcctcg gcggcgaagg
     2881 aggactcccg cgggggaaag acgacgatct tgccgccgga gaggcccttc cccctccagc
     2941 gtgagcgaga tgccgcgcgg cacgaacgcc ccgaagctct gcccggccga ccccgtcagg
     3001 tggatccgaa tgctctcctc cggcaggccc tcgctcccga acttgcgcgt gatctcactc
     3061 ccgaggatgg ccccgaccgt ccgatggacg ttccgaatgg gcagcttcag ctcggcgcga
     3121 gggatctcta tgccggcaag ggagggcact gcttcgccct tcacatcgcg ctgcaccggc
     3181 gggcaccagc ggatatgcac cgggaggtcg aggatccgct ggatcttggg atcggccagg
     3241 atctcgctgt ccagcgactc tccgatcgcg tgctcctgcg ccttcacccg gcgcgtggcg
     3301 accgcggggc ccacctccgg ccggtagagg atcttgctga agtccaggcc cctcgcctta
     3361 tagtgctcga tggccttgcg catgtcgagg aggtcgctgc gcccgaccat ctccgcgacg
     3421 gtgtggaagc cgagctgcgc catgatctcg cgcagctcct cagcgatgaa ctggaagaag
     3481 gtgaccacgt actccggctt gccctggaag cgcttgcgca gctctgggtt ctgcgtcgcg
     3541 atgcccatag ggcaggtgtc catgtgacag acgcgcatca ggacgcaccc catcaccacc
     3601 aggggcgccg tggcgaaccc gaactcctcg gcgccgagga gcgccgcaat ggcgacgtcg
     3661 cggccggtgc ggagctggcc gtccgtctgg acgatgatgc ggtcgcgcag gttgttcagc
     3721 accagcgtct gctgcgtctc cgccaggccc agctcccagg gaacgcccgc gtgcttgatc
     3781 gaggtgagcg ggctggcgcc cgtgcccccg tcatgcccgc tgatgaggac cacgtcgctg
     3841 tgggccttgg cgacgcccgc ggccacggtg cccacgccca cctcggaaac gagcttcaca
     3901 ctaatgcgtg ccctcgggtt cgcgcacttc aagtcgtaga tgagctgcgc caggtcctca
     3961 atggagtaga tgtcatgatg gggcggtggg ctgatcagaa ccacgcccgg cgtcgtgtgg
     4021 cgcaccttgg caatccaggg gtagacctta tgcgctggca gctcgccccc ttcgcccggc
     4081 ttggcgccct gggctatctt gatctgcagc tcgtcggcgc tcaccaggta ctcgctggtc
     4141 accccgaagc ggcccgaggc cacctgcttg atgcgactgc gcctgctgtc gccgttctcg
     4201 tccgggaaga agcggtccgg gtcttcaccg ccctcgccgg tgttgctcca cccacccagg
     4261 cggttcatgg caatagcaag cgtctcgtgc gcctctttgc tgatggagcc gtaggacatg
     4321 gccccggtgc agaatcgccg gacgatc
//
LOCUS       DAOSCV010000309         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12719_length_4344_cov_3.528095, whole genome shotgun sequence.
ACCESSION   DAOSCV010000309 DAOSCV010000000
VERSION     DAOSCV010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12719_length_4344_cov_3.528095"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..73)
                     /locus_tag="PLY56_08685"
     CDS             complement(<1..73)
                     /locus_tag="PLY56_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21596.1"
                     /translation="MKERPGAKGFTLIELLVVIAIIAI"
     gene            326..1669
                     /locus_tag="PLY56_08690"
     CDS             326..1669
                     /locus_tag="PLY56_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21597.1"
                     /translation="MAEVEECICIHPKKRRARWLLLAGACVVAGLALLWWRHINTVPR
                     YAMPRPALPQPNAFDYYLSAGDALDSLRGYPLVQSPSTSPEYLALVVEANKPALATFR
                     AGLRYSFLTPPPDPQALAYRFPYLESFERLGYLLWAESRASLAIDDVEGALESALLLI
                     RLGIDISRGGLLTHVQVGRAIEQRGLAQLAVVGKELDAQQCRKVLHRLRELEARRPPL
                     TEAIHRERDLLREFLWRLSCRQPDAIYALDTFLRRRWSSGPRLLYLINLRGAHDQLVQ
                     RADEAARLSALPAGQRAPLTPATSPLLERLDLVDRLHFALEGETILRARSRLLQTALA
                     LRAYRLEQGRSPDSLEDLVPRYLPSVPLDPYGEGGPMRYEPRPEPHAYSLGPDGTDDG
                     GRTLPWRSLTRANYPPEGDLKVILGEYDMWRSGSPRMPRSAFRDPPRPPHPAHPR"
     gene            1864..3672
                     /gene="lepA"
                     /locus_tag="PLY56_08695"
     CDS             1864..3672
                     /gene="lepA"
                     /locus_tag="PLY56_08695"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ21598.1"
                     /translation="MDNSQRHIRNFCIIAHIDHGKSTLADRILQFTGAISDRDMQDQV
                     LDTMDLERERGITIKMTAVRLTYRASDGEEYTLNLIDTPGHVDFTYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVNLALNNNLELIPVINKIDLPAADPERVKHEIEEALVLDAS
                     GAILASAREGIGTEEILEAVVQRIPPPSGDPDAPLRALIFDSHFDAYLGVVAYVRVVD
                     GCIRPGQPIRMMSNGKPFEVSTVGIFRPRMERVSELRAGEVGFLTAGIKSVSDCQVGD
                     TITDAKRPAATPLPGYKRAKPMVFCGLYPVDSRDYPELKDALAKLQLNDAALTFEPES
                     SAALGFGYRCGFLGLLHMEIIQERLEREFELDLVATAPSVVYRVTTTDGAVVNVDNPA
                     ALPPPTKIEKIEEPYVQATIIAPSDFIGPCMELCQDRRGVYRALEYPAPNRALIRYDL
                     PLSEILMDFFDQLKSRTKGYASFDYEFKGYEESSLVKLDICLNGDPVDALSFITHRER
                     AYPRGKALVERLKSILPRQQFEIRIQASIGNKVIAAERISALRKNVIAKCYGGDITRK
                     RKLLERQKEGKKRMKQIGSVEVPQEAFMSVLRIGEG"
     gene            3832..>4344
                     /locus_tag="PLY56_08700"
     CDS             3832..>4344
                     /locus_tag="PLY56_08700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ21599.1"
                     /translation="MRAGLLDKLMLDLCGDVAGLRVVDCGCGEGRFCRMLAARGAAHV
                     LGLDLCEAMIAAARELQSDNEEYRVADVQELSFLADETVDLAVSYLNQCDLPNIDANN
                     REVFRILRPGGRFIIANLHPMRSASGFWHRDSEGKKLHVILDRYFDEGERHWTMLGVN
                     FTNFHRTLATY"
BASE COUNT          767 a         1464 c         1313 g          800 t
ORIGIN      
        1 ggatggcaat gatggcaatg acgacgagca actcaatgag cgtgaagccc tttgcaccag
       61 gtcgctcttt catgaaactc cctcctgaag atcccggccg gaactcctca gcaccccgcg
      121 ctacctgctc gggggagcag gtgtaccctg cgtttcgctc gatcacagcc ggaagccctc
      181 aacgccctgt atagtgatag catacgacgc caggcaggcg cgttcctttc cgccacctgc
      241 cttccccttt cacgttggat ctaggccgcg atttgggagg gattcggccc ttggcggtgg
      301 aacagagagc gcggaagggc tccgcatggc cgaggtcgaa gagtgcatct gcatccatcc
      361 gaaaaagcgc cgcgcgcgat ggctcctcct ggctggcgcc tgcgtcgtgg ccggtctggc
      421 tctcctctgg tggcggcaca tcaacaccgt gccccgatat gccatgcccc ggcccgcgct
      481 tccccagccc aatgcgttcg attactacct cagcgccggc gatgctctcg actccctccg
      541 cggctatccg ctggtgcaaa gcccatcaac ctccccggaa tacctggcgc tcgttgtcga
      601 agcgaacaag ccggcactcg ccacgtttcg cgccggcctg cgctattctt ttctcacccc
      661 gccaccggat ccgcaggcgc tcgcgtaccg cttcccctat ctagagagct tcgagcgcct
      721 ggggtacctc ctctgggccg aaagccgtgc atccctcgcc atcgatgacg tcgagggcgc
      781 cctggagtcc gcccttctcc tgatccgcct gggtatcgat atctcgcgcg gcgggcttct
      841 cacccacgtc caggtgggtc gcgccatcga gcagagaggc ctggcccagc tggccgtcgt
      901 gggcaaggag ctggacgcgc agcaatgccg gaaggtgctg catcgcctcc gtgagctaga
      961 agcgcggcgc ccgccgctca ccgaggcgat ccaccgcgag cgagacctgc ttcgcgagtt
     1021 cctctggcgt ctgtcgtgcc gccagccgga tgccatctac gcgctcgata cctttctgcg
     1081 ccggcgttgg tcctcgggcc cgcggctgct ctaccttatc aatctgcgcg gcgcccacga
     1141 tcagttggtg cagcgcgcag atgaggccgc ccggctttcg gcgttgccgg ctggccagcg
     1201 tgctcctctc accccagcca cttcgcccct cctcgaacgg ctggatctgg tggatcgcct
     1261 ccacttcgca ctcgagggag agaccattct gcgcgcccgc tcgcgcctcc tacagactgc
     1321 gctggcgctc cgcgcctatc gcctggaaca gggccgctct cctgattccc tggaagatct
     1381 cgtcccgcgc tatcttccct cggttccact ggatccttat ggagagggcg gtccaatgcg
     1441 ctacgagccc cgccccgagc ctcacgccta cagcctgggg cctgatggca ccgacgatgg
     1501 ggggcgcacc cttccctggc gctcgctcac cagggccaac tatccccccg agggcgatct
     1561 caaggtgatc ctgggcgaat atgacatgtg gcgctctgga tcgccccgta tgcctcgctc
     1621 cgctttccga gacccgccgc gccccccgca ccctgctcat ccccggtaaa tcggacacgc
     1681 tcacacgcca tcgccttgcc gaatggcgcg atagcccaca ctttccgcca gcggtgcctt
     1741 ctgttcagcg catttggggc cggcgcggaa gcgccgccag cgcacccgcg agagggttca
     1801 agcgtttggt tcgttgacac tcctgcccgg gtctgttaga atctcttttg gactcatacg
     1861 cccatggata atagtcagcg acatataaga aacttctgca ttattgccca tattgatcac
     1921 ggtaagtcga ctctggccga ccgcatcctg cagttcaccg gcgccatctc ggatcgggat
     1981 atgcaggatc aggtgctcga tacgatggat ctggagcgcg agcgcgggat cacaatcaag
     2041 atgaccgcgg tccgcctcac ctatcgcgcc agcgacggcg aggagtacac actcaacctg
     2101 atcgacaccc ccgggcacgt cgacttcact tatgaagtct cgcgctcgct cgcagcgtgc
     2161 gagggcgccc tgctcgtcgt agatgcggct cagggagtgg aggcgcaaac cctcgccaat
     2221 gtgaacctgg cgctcaacaa taacctggag ctgatcccag tcatcaacaa gattgacctc
     2281 cccgcggccg acccggagcg cgtgaagcac gagattgagg aggccctcgt cctggatgct
     2341 tccggcgcga tcctcgcgag tgcgcgggag gggatcggca ccgaggagat tctcgaagcc
     2401 gttgtccagc gcattccgcc gccgtcgggc gatcccgacg cgccactgcg cgcgctcatc
     2461 ttcgactcgc actttgatgc ctacctgggc gtggtggcct atgtccgcgt tgtggatggt
     2521 tgcatccgcc ctggacagcc gatccggatg atgtccaatg gcaaaccgtt cgaggtgagc
     2581 accgtcggca tctttcggcc gcgcatggag cgtgtctcgg agctgcgcgc cggcgaggtg
     2641 ggcttcctca ccgccggcat caagtcggtg agcgactgcc aagtgggcga tacgatcacc
     2701 gacgccaaga ggcctgctgc caccccgctc ccgggctaca agcgtgccaa gccgatggtc
     2761 ttttgcggcc tctatccggt ggacagccgc gactacccgg agttgaagga cgcgctggcc
     2821 aagctgcagc tcaacgacgc cgcgctcacc ttcgagccgg agtcctccgc ggccctcggg
     2881 tttggctacc gctgcggctt cctcgggctc ctgcacatgg agatcatcca ggaacgcctg
     2941 gagcgcgagt tcgagctcga tttggttgcc accgccccga gcgtggtcta ccgcgtcacc
     3001 accacggatg gcgccgtggt gaacgtggat aacccggcgg cgcttcctcc gcccaccaag
     3061 atcgagaaga tcgaggagcc ctacgtccag gcgacgatca tcgcgccctc agatttcatc
     3121 gggccgtgca tggagctatg ccaggaccgc cgcggcgtct accgcgcgct ggagtatccc
     3181 gccccgaacc gggcgttgat ccggtatgac ctgccgctct cagagatctt gatggacttc
     3241 ttcgaccagc tgaagagccg gacgaagggc tacgcatcct tcgactacga gttcaagggc
     3301 tatgaagagt cgagcctggt gaagctggac atctgcctga atggcgaccc ggtagatgcg
     3361 ctctccttca tcacgcaccg cgagcgcgcc tacccgcgcg ggaaggccct cgtggaacgc
     3421 ctgaagtcga tcctgccccg ccagcagttc gaaatccgca tccaggcatc cattggcaac
     3481 aaagtgatcg cggccgagcg gatcagcgcc ctgcgcaaga acgtcatcgc caagtgctat
     3541 ggcggcgaca tcacccgcaa gcgcaagcta ctcgaaaggc agaaagaggg caagaagcgc
     3601 atgaagcaga tcggtagcgt cgaagtgccc caggaagcct tcatgagcgt gctccgcatt
     3661 ggcgagggat aggcatcagc ccggaaaaca ggcccgtcat ccgggcgcgg gcgacgccgg
     3721 cgaccgctgc gagttgctga aaggatcgtg atggatagag ccgacctcca acgcgaatgg
     3781 cacgcgctat cgaaaccatg gatcgatgaa gtgcggtttg ggaggaacgc agtgcgcgcc
     3841 gggctgctgg acaagctgat gctcgacttg tgcggcgatg tggccgggct gcgggtggtt
     3901 gattgcggat gcggcgaggg aaggttctgc cggatgctcg ccgctcgcgg cgccgcgcac
     3961 gtgttggggc tggacctgtg cgaagcaatg atcgccgcag cacgggagct gcagtcggac
     4021 aacgaagagt accgcgtggc tgacgttcag gagttgagct tcctcgcgga cgaaaccgtt
     4081 gatctcgccg tgtcttacct gaaccagtgc gacctgccga acatcgatgc caacaaccgc
     4141 gaggtgtttc gcatccttcg gccgggaggc cgcttcatca tcgccaatct gcacccgatg
     4201 cgctccgcgt ccggtttctg gcatcgcgac agcgagggca agaagctgca tgtgatcctc
     4261 gaccgttact tcgacgaggg cgagcgccat tggacgatgt taggcgtcaa cttcaccaat
     4321 ttccatcgca cactcgccac ctac
//
LOCUS       DAOSCV010000310         4329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12781_length_4329_cov_3.620730, whole genome shotgun sequence.
ACCESSION   DAOSCV010000310 DAOSCV010000000
VERSION     DAOSCV010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12781_length_4329_cov_3.620730"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLY56_08705"
     CDS             complement(<1..842)
                     /locus_tag="PLY56_08705"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ21600.1"
                     /translation="MLRVIPLGGVTEIGKNMTIVEQDDDILIVDCGLMFPENDMLGVD
                     IVINDFRYLIERADKVRGVILTHGHEDHIGALPYLLRDLNVPIWGTRLTLGLVDAKLS
                     EWGLEEQATKHVIEAGETVTIGPFTIDTMHVGHSIPDGIGLGIHTKEGIIVHTGDFKF
                     DPTPVDGRMTDFAGFERLGNQGVLLLITDTTNVERPGYVPSERIVGQTFYDVFQRAER
                     RVIIATFASNIHRIQQAIQVSADFGRKVAILGRSMAANTDIASQLGYLDVPADTRIAV
                     PEID"
     gene            1233..2030
                     /gene="thiD"
                     /locus_tag="PLY56_08710"
     CDS             1233..2030
                     /gene="thiD"
                     /locus_tag="PLY56_08710"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOJ21601.1"
                     /translation="MRIALTIAGSDSGGGAGIQADLKTFAAFGVFGTSAITAITAQNT
                     LRVERADALDPTLVGAQIRAVATDLHPHAAKTGMLANAAIIDTVIACVTEFNIHPLVV
                     DPVMVASSGDVLLEPEAVVALRSLLLPLADLITPNLAEAEVLTGQPVRDESEMRAAAI
                     ALVEMGASAALVKGGHLSGDEMVDLLFDGREFHALRARRLPADNPHGTGCTLSAAIAA
                     GLARGVSLPLAVSRGVEYVRRAIETAPGLGRGRGPLNHFPESSPTRE"
     gene            2969..4207
                     /locus_tag="PLY56_08715"
     CDS             2969..4207
                     /locus_tag="PLY56_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOJ21602.1"
                     /translation="MSDRAHETPASVRPADHPPVSEGIKPSRPVEALVAPAPSQPSEH
                     PGPAATVASLGAGSQPAEPRLALLNLVTPPEELILDPTAVPVASATRVAASDRPAGPA
                     PVETAWEARGAVLEDILAASRNRSAQGGESMLTADPMQTEVPPAHSAPDGVADGALRF
                     VEGMEPARIPAGDESARAAVAPRQRELTLLRSTIPAQNLLHPIPEDAHNVEAAQESDL
                     PAGIPAVESGESAATGDTIGLRPEQGVLHSPQVTSASPSGAEPARAVAPTRYEGLALD
                     GPAQQIVTAAHLRLRGDGGTVEMRLDPPELGQVRLQVTLQDGVLVAHLGVETAAACQL
                     LEGQAAALRSALLDCGLPVQSLEISMGLEHSSGHPAHANQEETPRWGQQSPPEGVPVS
                     PASPAPGRSSHRVAGIDYFA"
BASE COUNT          756 a         1471 c         1414 g          688 t
ORIGIN      
        1 aaatcgatct ccggcaccgc gatgcgggta tcggcaggca cgtccagata gccaagctgg
       61 gaggcaatgt cggtgttggc cgccatgctc cgccccagaa tcgccacctt gcgcccgaag
      121 tcggcggaga cctggatcgc ctgctggatg cggtgaatgt tcgacgcaaa cgtggcgata
      181 atcacccggc gctccgcgcg ctggaagaca tcgtagaagg tttgcccgac gatccgctcc
      241 gagggcacat agcccggccg ctccacgttg gtcgtatcgg tgatcaggag gagcacgccc
      301 tgattgccca ggcgctcgaa gccggcgaag tccgtcatcc gaccatccac cggggtggga
      361 tcgaacttga agtcgccggt gtggacgatg atgccctcct tggtatggat gccgaggccg
      421 atcccatcag ggatgctgtg cccgacgtgc atcgtgtcga tggtgaacgg cccgatcgtg
      481 acggtctcac ccgcctcgat gacatgcttg gtcgcctgct cctccaggcc ccactcggag
      541 agcttggcat ccaccaagcc aagggtgagc cgcgtgcccc agattggcac gttcaggtca
      601 cgcaggaggt acggcagcgc gccgatgtga tcctcgtggc catgggtcag gataacgccg
      661 cgcaccttat cggcacgctc gatgaggtag cgaaaatcgt tgatgacgat gtcgaccccg
      721 agcatatcgt tctcgggaaa catgagcccg cagtcgacga tcaggatgtc gtcgtcctgc
      781 tcgacaatcg tcatattctt cccaatctcg gtaacacccc cgagcgggat tacacgaagc
      841 ataaagtcct cttggcggcc gtagtgagtt gtcaggcgga ggccggccag gctccgcccc
      901 ggcgttcgcg ttggggatgg catcgtgagg taaggtcgcc acgctgagcg tagcgaaaca
      961 cccccgaaag ggagatgctt ggctgcgccc aggatgacgg gccacgatgg cgtccgaacc
     1021 caaatggaac acccctgcgt ccctcggatc tcggacggca ctgccgaaga cgagacgggg
     1081 aacgcatgcc ttctcgttcc tctgtaacaa ttatagcgga tcaccccatg cggatcaaca
     1141 gaagcctccc gcctcacgaa cgcgcgcgct gcgtccaacg agtggttgac cgccggcctc
     1201 agagatgcta tgattgggcg cggagagaga tgatgcgcat cgctttgact atcgccgggt
     1261 ccgactctgg tggaggcgcc ggcatccagg ctgatctcaa gactttcgcc gcgttcggcg
     1321 tcttcggcac ctccgcgatc accgccatca cggcccagaa caccctccgg gtggagcgcg
     1381 cggatgccct cgatccgacg ctggtggggg cacagatccg ggccgttgcc accgacctgc
     1441 acccgcacgc cgccaagacg ggcatgcttg ccaacgccgc catcatcgac acggtgatcg
     1501 cgtgcgttac cgagttcaac atccaccccc tggtggtcga tcccgtaatg gtggcctcgt
     1561 ctggcgacgt cctgctggaa cccgaggcgg tcgtggcact gcgttcgctg ctactcccgc
     1621 tcgccgacct gatcaccccg aacctggccg aagcggaggt actcacgggc cagccggtga
     1681 gagacgagtc ggagatgcgc gcggctgcta tcgcgctcgt ggagatggga gccagcgccg
     1741 cgctcgtcaa gggagggcat ctctccgggg atgagatggt cgaccttctc tttgatggcc
     1801 gcgagtttca cgcattgcgc gcgcgccgcc tcccggcaga caacccgcac ggcacgggat
     1861 gcacgctctc ggcagccatc gccgccgggt tggcgcgcgg cgtctcgctt cctctcgccg
     1921 tttcgcgggg cgtggagtat gttcggcggg cgattgagac ggcaccgggc ctgggccgcg
     1981 gtcgcggtcc tctcaaccac ttcccggaga gctccccaac aagggagtag gccggtgaac
     2041 aggcgcagcc aggcgcgggc tcagcagccc cctccgggcc tagccgccgt gtgctgcagc
     2101 tgccccacct atcgcgttaa gctccgctcc ttccatgccg aattcggagg tggcggtccc
     2161 tctggagcgc cggtgagatt tcaaccgaag gagcatgcga gtggtgaccg tgccgataga
     2221 gcctcccgta tccggccagc ccgtcccttc cgcgctgccc gcgcctgttg cgcccgatgc
     2281 cggtttctcg cgattgcttg aggagaccct cgcgcagcct gtagtggcgg atcccttacc
     2341 catcccagaa cccgtcgtga gtgaggacgc gccctccact cacgcgacag tcgatgggat
     2401 cccccatcag gccggccagg cggtgatctg cctccgtgaa gggcaaggcc aaccggtacc
     2461 ggatgccgcg cggcccgtgg tcagttccga gagcgcccca cctgaccagg aggtggacgc
     2521 ccaggcctct gatccacccc tccgtgcgga gcaagagccc gaggaggggg cggagcacgc
     2581 tctctatccg acactattgc cggcgctgat cccccaaccc gcccccgagc cggctgcgcc
     2641 tgcggcaaac cccactgcgg acgggtcgtc agatcgcctg gtggcgcgcg cggcggatgc
     2701 cgtacgcccg catcacccgt gggagaacac cgacggccgt ccgatgccgg tcgcggaagc
     2761 gggaacaagc gcaccggctc atgagtctcg cgccagtgcc gacgccgagg gtgcaggagc
     2821 ccctctccca ccccaggcac cagctccagc gccggcagcc cggatcgatt ttggtagcga
     2881 gagcccggcg gttccagaaa ccgcttcggt ttacgataca tctcaaccca cggatagcgc
     2941 gcgagaggca actccgcagc agaccagaat gtctgatcga gcgcatgaga caccggcctc
     3001 cgtccggccg gcagaccatc ccccggtgag cgagggcatc aagccctcgc ggccagtgga
     3061 agcactcgtc gcgcccgcgc cgtcgcagcc atccgagcat ccggggccgg ccgccacagt
     3121 ggcatccctg ggcgcgggca gccagccagc ggagccccgt ctcgcgctgc tcaacctggt
     3181 taccccccca gaggagctga ttctcgatcc gaccgcggtt ccagtggcga gcgccacgag
     3241 agtggccgcc tccgataggc cggcaggtcc cgcccccgtg gaaacggcgt gggaagcacg
     3301 gggggcagtc ctggaagaca tcctcgccgc ttctcgcaat cgctcagccc agggcgggga
     3361 gagcatgctg acggcagatc caatgcagac cgaggtgcca ccggcacaca gcgcccccga
     3421 tggcgtggcg gacggcgcgc tccgcttcgt ggagggcatg gagcccgctc ggattccagc
     3481 tggcgacgag tcggcacgag cggcggtcgc accgcgccaa cgggaactca ccctgcttcg
     3541 cagcacgatc cccgcgcaga acctgctgca tccgattccc gaagacgcgc acaacgtcga
     3601 agcggcccaa gagagtgact tgcccgccgg catcccggcg gtggagagcg gcgagagtgc
     3661 agctacgggc gataccatcg gcctgcgtcc agagcaggga gtcctccatt cgccgcaggt
     3721 cacgagcgcc agccctagtg gggccgagcc agcgcgcgct gttgcaccca ctcgatatga
     3781 gggactcgcg cttgacggtc ccgcgcaaca gattgtgacg gcggcgcatc ttcgcctgcg
     3841 aggcgatggt gggaccgtgg aaatgcgcct cgatccccct gaattgggtc aggtccgtct
     3901 ccaggtgacg ctgcaggatg gcgtcctggt tgcccacctg ggcgtagaaa cggcggcagc
     3961 ttgccagcta ctcgagggcc aggccgcggc gcttcgctcc gcgctcctcg attgtgggct
     4021 gccagtccag tcgctggaga tcagcatggg cctggagcat tccagtgggc acccggccca
     4081 cgccaatcaa gaggagacgc cccgttgggg ccagcaaagc ccccccgagg gtgtgccagt
     4141 ctccccggct tcaccagcgc cggggcggtc ctcgcaccga gtcgcgggga tcgactattt
     4201 cgcatgagac gccgccaggt cgaggtgcta aagccaaggc gctcgtctgc cgatacagat
     4261 aaggggcacc ttggtgttcg agtagggaga gcatgggcag gcgcgggtag gcgagcagga
     4321 tgcgccgga
//
LOCUS       DAOSCV010000311         4324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12803_length_4324_cov_2.602015, whole genome shotgun sequence.
ACCESSION   DAOSCV010000311 DAOSCV010000000
VERSION     DAOSCV010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12803_length_4324_cov_2.602015"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..605
                     /locus_tag="PLY56_08720"
     CDS             3..605
                     /locus_tag="PLY56_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21603.1"
                     /translation="MIELLVVIAIIAILAAILFPVFARARENARKSNCQSNIKQLATA
                     QLQYAQDYDEMVLRAIYRDPASNYLWSRALSPYIKNTGVYKCPSSANHRYTEVSGADR
                     GYMSVGYNWALADVDVKLSQFEHPTEIVLFGDTYNGDASVGYRGYEFAMTAARMCDTY
                     EALSARHGDGANLGFADGHVKWVPRNTINAKTGLRFYAPW"
     gene            complement(707..1723)
                     /locus_tag="PLY56_08725"
     CDS             complement(707..1723)
                     /locus_tag="PLY56_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HOJ21604.1"
                     /translation="MPESLNILFTAKEQVTLERSPVAEPGPGEVLVRTTWTLVSTGTE
                     CICDGQLYEPGTHWERYARYPMAAGYSHAGVVERIGPGVTDLAPGDRVASFANHRQWV
                     CAPAGQFLRVPEGVSDEEATFYALATVAQNAVRRAAHEMGDIVAVIGLGILGQLVVQY
                     ARVMGAWEVIAIDPSETRLGMAAAHGATHTLCCRADAARDAIAEITRGRMADVVYDVT
                     GFPEAFAPAQRLARRFGRLVLLGDSWAPSEQRLTQDFLWRGLQVIGAFSGDPPATADD
                     HHFWTRPHMCQLFFDYLRRDQMRVRDLITHRFHPEQAPEVYAMLLRDRSSTLGVLFDW
                     RDVA"
     gene            complement(1736..3505)
                     /locus_tag="PLY56_08730"
     CDS             complement(1736..3505)
                     /locus_tag="PLY56_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ21605.1"
                     /translation="MKSSDKPGILLRLALAAALCLACLAPARAAGVPVSELKAAYRDG
                     QIFLTWREEATPEGTTFNVYRHHQPITEANLAKATKVGHHVERHSARDWWQDPASFAP
                     DTPAAPPAGFRIQAGAAPLDPSGGLFVHTVTPQTAGASYFAVISSSATGVEDTTLVPG
                     VNTLIEPVVGRVALAQPIWQEDGPAPAPGSGKGKSLTFVLHGRGGGKTAGPGAQRVNY
                     LAFGDAKQGWREGLPFKFLVSISDTTVRITPCDRHWAGRPVTESPDRRDHCPAISSFW
                     YGYNSRLYETTRTTKSVVPNYTEEQILWMMRWAQAYLGTDPNRTYLTGGSMGGSGAIS
                     LAMHYPQHFAAVMATVPVVSFTTRGGGRGSLARLDCACGPVDATTVNHEGVPLLEHMN
                     GELQAARAKADLPYLFVTHGRTDRSIPWANNPPFYRAMNAARQGLSVYWNNEDHAGVA
                     RACPDDVKAWTPLLERFALNRSFPVFTNCSDNKDPGDGDPARGDIIGWMNRGLDWKDV
                     VDTPTLYAITVIAAHPEIRYPVTVDLTLRRLQQFKVRAGEVVKLSVGGQPPIPLRVDE
                     KGLITISNVRIESAEGTRIQLAR"
     gene            complement(3739..>4324)
                     /locus_tag="PLY56_08735"
     CDS             complement(3739..>4324)
                     /locus_tag="PLY56_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21606.1"
                     /translation="MPDMPITLYDRGSAIAAVGDTEVLATIGAPYFNRHWDGEHHFFY
                     LPPDKPTNRPAVTVSGPVAHISHPLFTSYYNHAQVPMRTLIGHLIARLMPNEMVKTEG
                     MPSFGRVTVTRQPNRRMVHLLSYVPERRGATIDMIEEPVELRDIAVALREDGQAVSRV
                     YLAPTGQDLPFEVAGGYVKTRVPVVPGYAMVVFE"
BASE COUNT          720 a         1420 c         1464 g          720 t
ORIGIN      
        1 ccctgatcga gttgcttgtt gtcattgcga ttatcgccat cttggcagcc atcctctttc
       61 cggtttttgc ccgggcgcgg gagaatgcgc ggaagagcaa ctgtcagagc aacatcaagc
      121 aactcgcgac ggcccaactg cagtacgcgc aggactacga tgagatggtg ctgcgagcta
      181 tctaccgcga ccccgcaagc aattacctct ggtcgcgggc gctcagcccc tatatcaaga
      241 acacaggggt ctacaagtgc cccagctccg cgaaccaccg ctacaccgag gtttcgggag
      301 cggatcgggg ctacatgtcc gtgggctaca actgggcgct ggccgatgtg gatgtgaaac
      361 tctcgcagtt cgagcacccg acggagatcg ttctgttcgg cgatacctac aatggcgatg
      421 cctcggtcgg ctatcgcggc tatgagtttg ccatgacggc ggcccggatg tgcgacacgt
      481 acgaagcact gagcgcgcgg catggggatg gcgccaacct tggcttcgcc gatgggcatg
      541 tgaagtgggt gccccgaaac acgatcaacg ccaagacggg cttgcgcttc tacgcgccct
      601 ggtaggccgg acacgcgggg cagtcggagc cgccccgact gccccgctgc ctccgttcat
      661 ctgaggataa cgaaaacccc gcgcggcagg cgtcgtgttg cggttttcag gcgacgtcgc
      721 gccagtcgaa gaggacgccg agggtggagg aacgatcgcg caggagcatg gcatacacct
      781 caggggcctg ctccggatgg aagcggtggg tgatcaggtc gcgcacgcgc atctggtcgc
      841 gacggagata gtcgaagaag agctggcaca tatgggggcg ggtccagaag tggtggtcgt
      901 ccgcggttgc cggcgggtcg ccgctgaagg cgccgatcac ctggaggccg cgccatagga
      961 agtcctgagt gagccgctgc tccgagggcg cccaggaatc gcccagaagg accaggcgcc
     1021 cgaaccgccg ggcgagtctc tgcgctggcg cgaatgcctc cgggaagccg gtcacatcgt
     1081 agacgacatc cgccatgcgc ccgcgcgtga tctcggcgat ggcatcacgt gccgcatcgg
     1141 cgcgacagca aagggtgtgc gtggcgccgt gcgcggcggc catgcccagc cgggtctccg
     1201 aaggatcgat cgcgattacc tcccacgcgc ccatcacccg cgcatactgc accacgagct
     1261 gccccagaat gccaaggccg atgaccgcca caatgtcgcc catctcgtgc gcggcccgcc
     1321 ggaccgcatt ttgcgccacg gtggcgaggg cgtagaaggt ggcctcctcg tcgctcaccc
     1381 cctccggcac gcgaaggaac tgcccggcgg gcgcgcagac ccattgccga tggttggcga
     1441 agctcgccac gcggtcgccc ggcgcgagat cagtgacgcc cggcccgatg cgctccacca
     1501 ccccggcgtg gctgtagccc gcggccatcg ggtagcgcgc gtatcgctcc cagtgcgtgc
     1561 ccggctcata cagttgtccg tcgcagatgc actcggtgcc agtgctgacc agcgtccagg
     1621 tcgtgcgcac cagcacctcg cccggacccg gctcggcgac gggcgatcgc tccagcgtaa
     1681 cctgctcctt cgcggtgaag aggatattca gcgattccgg catggcccct cccagtcatc
     1741 gcgcgagctg gatgcgggtt ccctccgcgc tctcgatccg aacgttcgag atggtgatca
     1801 agcccttctc atccacccgc agcggaattg gcggctggcc tcccacggag agcttcacca
     1861 cctccccagc gcggactttg aactgctgta gccggcgcaa ggtgagatcc accgtcaccg
     1921 gatagcggat ctccggatgg gccgcaatga cggtgatcgc gtagagggtg ggcgtgtcca
     1981 ccacatcctt ccaatcgagg ccccgattca tccacccaat gatgtctccc ctggccgggt
     2041 cgccatcgcc gggatccttg ttatcggagc agttcgtgaa gacggggaag ctccggttga
     2101 gcgcgaatcg ctccagaagc ggggtccatg ccttcacatc atcggggcag gcgcgggcca
     2161 ctccggcatg gtcctcgttg ttccagtaga ccgagaggcc ctgccgggca gcgttcatcg
     2221 cccggtagaa gggcgggttg ttggcccagg ggatcgagcg gtcggtgcgc ccgtgagtga
     2281 cgaagaggta ggggagatcc gctttcgcgc gcgccgcttg gagctcgccg ttcatatgct
     2341 ccagcagggg cacgccctcg tggttgaccg tcgtcgcgtc caccggcccg caggcgcaat
     2401 cgaggcgggc caggctgccg cgccccccgc cgcgcgtggt gaaggagacg accggcacgg
     2461 tggccatcac cgccgcgaag tgctgcgggt agtgcatcgc cagcgagatg gcgcccgatc
     2521 cgcccatgga gccgccggtg agataggtgc ggttgggatc cgtgcccaga tacgcctgcg
     2581 cccagcgcat catccagagg atctgttcct cggtgtagtt tggcaccacg ctcttggtcg
     2641 tccgcgtcgt ctcgtagagc cggctgttgt agccgtacca gaagctgctg atcgcggggc
     2701 agtggtcgcg ccggtccggc gactcggtga cgggccgtcc cgcccaatgg cgatcgcagg
     2761 gcgtgatgcg caccgtggta tcactgatgg agaccaggaa cttgaacggc aggccctcgc
     2821 gccatccctg tttagcatcg ccgaaggcca ggtagttcac ccgttgcgcg cccgggcccg
     2881 cggttttgcc gccgccgcgg ccatgcagca cgaaggtgag gctctttccc ttccccgatc
     2941 cgggcgccgg cgccggcccg tcctcttgcc agatgggctg cgccagcgcg acacggccga
     3001 caaccggctc tatcagcgtg ttcactccgg gaacgagggt tgtgtcttca accccagtgg
     3061 cgctggagga tatgaccgcg aagtaggatg caccggccgt ctggggggtg acggtatgca
     3121 cgaagagacc ccccgaggga tcgagaggag ccgcgcctgc ctggattcgg aagccggccg
     3181 gcggcgccgc aggcgtgtcc ggggcgaagg atgccgggtc ttgccaccaa tcgcgcgcgc
     3241 tgtgccgctc cacatggtgg cccaccttcg tcgcctttgc caggttggcc tccgtgatcg
     3301 gctggtggtg ccggtagacg ttgaaggtgg tgccctccgg ggtggcttcc tcgcgccaag
     3361 tgaggaagat ctgcccgtcg cggtaggcag cctttagctc agagacgggc acgcccgccg
     3421 cgcgcgcggg cgcgaggcag gcgagacaca gcgccgccgc gagggccagc cgcaagagga
     3481 tgccaggctt atccgatgac ttcatctgat ctcgtccgtc ccaaagatcc gttcggaggc
     3541 atcaccgccg atgccccccc gcgggatgcc cggagcgagg gcatcccgcg gcactccggg
     3601 caaccgtgcg cggcgatccg gcaacgcgag ccatcgtgct ccccggtggg gatgctcgcc
     3661 catccgacgg cgctctcccc accgaggctc catcccttgc tgataaggat ggcttttcca
     3721 gcacacgcag ccgtgccgct actcgaagac gaccatcgca tacccgggca cgacgggaac
     3781 ccgcgtcttg acataaccgc ccgcgacctc gaaggggagg tcttgccccg tgggcgccag
     3841 gtagacacgg ctcacggcct gcccatcctc gcgcaaggcg acggcgatgt cgcgcagctc
     3901 caccggctcc tcgatcatgt cgatggtggc accccggcgc tccggcacgt aggagaggag
     3961 atggaccatc cggcggtttg gctggcgcgt cacagtcact cgcccgaagg agggcatccc
     4021 ctcggtctta accatctcgt tcggcatcag ccgggcgatg agatggccga tcagggtgcg
     4081 catcggcacc tgggcgtggt tgtagtagga ggtgaagagg gggtggctga tatgcgccac
     4141 cgggccactc accgtgacgg ccgggcggtt ggtcggctta tcgggcggga ggtagaagaa
     4201 gtggtgctcg ccgtcccagt gccggttgaa gtaaggagcg ccaatcgtgg ccagcacttc
     4261 ggtatcgccg acggcggcga tggcgctgcc gcggtcgtag agggtgatcg gcatatcagg
     4321 catt
//
LOCUS       DAOSCV010000312         4322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12810_length_4322_cov_3.365831, whole genome shotgun sequence.
ACCESSION   DAOSCV010000312 DAOSCV010000000
VERSION     DAOSCV010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12810_length_4322_cov_3.365831"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4166
                     /locus_tag="PLY56_08740"
     CDS             <1..4166
                     /locus_tag="PLY56_08740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ21607.1"
                     /translation="IDRWRVDGPNPRPFTVGARAKAEVTVNVVAGRGIYAAHYPVHAY
                     AEFTEGGQPLRAHAVLVIEVARAAVEVAKPRPPAVAQVAALSAEAPVKVDGDLSEWTN
                     AIPVPLDSSHASVGPVEPGDFGAVLTALHDGERLYLGARVSDDEISATDRASRDFMDS
                     DYIRLYLSARSPLERSASGLTEEDLVLAINPFGGERPLVKVPDYGLKTRPLPDPGAVQ
                     VAARRTPGGYEAEIALPLSLIGPDVKEGKRLGFNVMLGDADGGRRVAELTLGHRDRDY
                     WLSPSAYVPLELSSRTRGDGTLQLPVNVIAGRGATRLDRLTGFRVCIAREGKPLIEKP
                     LGWTGHDRETGTSFTPGTASRPDARPCITIHPPYRGGAGTSWADARFTLPNVKPITLR
                     FATAIRDHMPGEGVSDGVEWRVYVAEESAPSGQPGTTAEGAGFVQLFERFSLSKTWEP
                     ATVDLSAYAGKTVTLRLWNGPGPKGNTICDSGYWAEPTLVVGKPPAEETPQARASRRQ
                     KAEAKAKQARAGKTDHLSWRTQSRAGLFGIALVPGPRGLVDGVLAVAGERETVSFEGF
                     LIEVAGERLGDWRSAASVEEVKILPPTKANPNAVTIRHTVLLNEARIPVDAVLRPVAG
                     SVRLSFSMPGVKRDRRGQPRFTLLGIGPASRKARRVYAGFGNVIQEPGRFTLHGSGFQ
                     LSTRHVGIDFENGVSLAQACDVFPDRFEVDPEKRLYSLQTHHDASFFLVPSARGAFAA
                     ARVYRDLAGFKPAGGVAKLKGKMCIDWWGGHEIAADIRRAAAYGLTDSVFIKHAWQRW
                     GYDYRLPDIYPPDCDPVIWNDWVSACRENGILFGVHDNYIDFYPDAEGFSYKHIVFNE
                     DGTPQRAWFNKGPQAQSYRWLPHAFKPWLERNMKLIKEGFAPTASFVDVFTAHAVIDY
                     YDEEGNFYTKMKCAKHWGECFDYIREVLGDNAPTISEAGHDALIGHVDGVQSDHYSAK
                     TWNWPCTDAERVPWHDMATHGAMILFAGGLGQRYAGDEPYASWGSDDYFSNTVIGGRN
                     PMCTGPCTRDTVKTYWLLHDVCKELAHQSLETHEFVGDDIHRQHTTFDGGGKVWTNRG
                     KTPWEVGGFMLPRYGFLAKAGQTMAAIFAREGLSVALARSPGVTFVDARPLSLDVASR
                     APVKTRVLGARHLGGGQVEVEFEWEVLRPLPEEYRPFVHVSHPKAVAQGSDRIAIHAP
                     SGLEPEKLQQVGTHRCTIRFTFASAPYDGDYYLRYGIYSPKRGNRLLPLADIDGTRVR
                     GGIFTVKRVQGAIESVKYAPERPGPDAPKENAEGKKIDFGSVVTNGAFRLLHSGNEWR
                     LYPLQGSFPFQADLRLATLGAKGKRVTSVDGLDQSGKPLGAVTFEQKGDEVHLDLDAA
                     AFSYRIVLR"
BASE COUNT          723 a         1423 c         1498 g          678 t
ORIGIN      
        1 tcatcgaccg gtggcgcgtg gatgggccca acccgcgtcc tttcacagtg ggagcccgcg
       61 cgaaggccga ggtgacggtg aacgtggtcg ccggcagggg catctacgcc gctcactacc
      121 cggtccacgc ctacgcggag ttcaccgagg gtgggcagcc cctgcgcgcg cacgccgtgc
      181 tcgtcatcga ggtggcgcgc gctgctgtgg aagtggcgaa gccgcgcccg ccggcagtcg
      241 cccaggtggc ggcactgagc gcggaggcgc ccgtgaaggt ggatggcgac ctctcggaat
      301 ggaccaacgc gattcccgtg cccctcgata gcagccacgc gagcgtgggc cccgtcgagc
      361 cgggcgattt cggcgccgtg ctcaccgcgc tccatgacgg cgagcggctt tacctcggcg
      421 cgcgcgtctc ggatgacgag atcagcgcca ccgaccgcgc gagccgcgat ttcatggata
      481 gcgattacat ccgcctctac ctctcggcgc gctccccgct tgagcgctct gcgagcgggc
      541 ttaccgagga ggacctggtc ctcgccatca atcccttcgg cggcgagagg ccgcttgtga
      601 aagtgcctga ctacggcctc aagacgcgcc cgctcccgga tccgggcgcg gtgcaggtcg
      661 ccgcccgacg cacgcccggg ggctatgagg cggagatcgc cctccccctg tcgctgattg
      721 gccccgacgt gaaagaggga aagcggcttg gattcaacgt gatgctgggc gacgccgatg
      781 ggggccggcg cgtcgccgag ctcacgctcg gacaccgtga ccgtgactac tggctctcgc
      841 cctctgccta tgtgcctctg gagctctcct cgcgcacgcg cggcgatggc acgctgcagc
      901 ttccggtgaa cgtcatcgcc gggcggggcg ctacccggct ggaccggctc acgggcttcc
      961 gggtgtgcat cgcccgtgag ggaaagccgc tgattgagaa gccgctcggg tggaccgggc
     1021 acgaccgaga aacgggaacg agcttcacgc caggaacggc atcgcgcccg gatgcccggc
     1081 catgcatcac gattcaccca ccctatcggg gtggcgccgg cacctcgtgg gcagacgcgc
     1141 gcttcaccct cccgaacgtg aagccgatca cgctccggtt tgccacggcg atccgtgacc
     1201 acatgcccgg cgagggcgtg agcgatggcg tcgagtggcg cgtctatgtg gcggaagaga
     1261 gcgcgccatc cggccagccc ggcacgacgg cggagggggc cgggtttgtc cagctgttcg
     1321 aacgcttcag cctctccaag acgtgggaac cggccacggt cgacctttcg gcctacgcgg
     1381 gcaagacggt gacgctgcgc ctctggaacg gccccggccc gaaaggaaac acaatctgcg
     1441 attccggcta ctgggcagag ccgacgctgg tggtgggcaa accgcccgca gaggagacgc
     1501 cccaggcgcg cgcctcacgg aggcagaagg ccgaggccaa ggcgaagcaa gcccgcgccg
     1561 ggaagactga ccacctctcg tggagaacgc agagccgcgc cggcctcttc ggcatcgcgc
     1621 tagtacctgg cccgcgcggc ctggtagatg gcgttctcgc cgtggccggc gagcgtgaga
     1681 cggtttcgtt cgagggcttt ctgatcgagg tggccggcga gcgcctgggc gactggcgct
     1741 ccgccgcgag cgtagaggag gtcaaaatcc tgcctcccac gaaggcgaac ccgaacgccg
     1801 tgacgatccg gcacaccgtc ctcctaaacg aggcgcgaat tccggtggac gcggtgctcc
     1861 gtccggtggc gggtagcgtc cgcctctcct tctcgatgcc gggcgtgaag cgcgaccggc
     1921 ggggccagcc gcggttcacg ttgcttggga tcggcccggc cagccggaag gcgcggcgcg
     1981 tttacgccgg tttcgggaac gtgatccagg agccggggcg gttcaccctg cacgggagcg
     2041 gcttccagct gagcacccgc catgtgggaa tcgatttcga gaacggcgtc agcttggcgc
     2101 aggcgtgcga tgtcttcccc gaccggtttg aagtcgaccc ggagaaacgg ctctactcgc
     2161 tccagacgca ccacgacgca tcgttcttcc tggtgccctc ggcacgcggg gcgttcgccg
     2221 cggcgcgcgt ctaccgcgat ctcgccggat tcaagccagc aggcggcgtc gcgaagctga
     2281 aggggaagat gtgcatcgac tggtggggcg ggcacgagat cgccgcggat attcgccggg
     2341 ccgccgccta cggcctcacc gactccgtct tcatcaagca tgcctggcag cgctggggct
     2401 atgactaccg cctgcccgat atctatcccc cggactgcga cccggtgatc tggaacgact
     2461 gggtgagcgc gtgccgcgaa aacggcatcc tctttggcgt gcacgataac tatatcgact
     2521 tctacccgga tgccgagggc ttcagctaca agcacatcgt cttcaatgag gacggcacgc
     2581 cccagcgcgc gtggttcaac aagggccccc aagcgcaaag ctaccgctgg ctcccccacg
     2641 cgttcaagcc gtggctggag cgcaatatga agctgatcaa ggagggcttc gcgcccactg
     2701 cctcgtttgt cgatgtcttc accgcgcatg cggtgatcga ctactatgac gaggaaggca
     2761 acttctacac gaagatgaag tgcgcgaagc actggggcga gtgcttcgat tacatccgcg
     2821 aggtcctggg agataacgcg cccacgatct ccgaggccgg acacgacgcg ctgatcgggc
     2881 acgtcgacgg cgttcagtcg gaccactact cggcgaaaac ctggaactgg ccctgcaccg
     2941 atgccgagcg cgttccctgg cacgacatgg cgacgcacgg ggcgatgatc ctctttgccg
     3001 gcggcctggg ccagcgctac gcgggcgacg agccctacgc gagctggggg agcgacgact
     3061 acttctccaa cacggtgata ggcgggcgaa acccaatgtg caccgggccg tgcacccgcg
     3121 acactgtgaa aacctactgg ctgttgcacg acgtgtgcaa ggagctcgcc caccagtcgc
     3181 tcgaaacgca cgagttcgtg ggcgatgaca ttcaccgcca gcacaccact tttgacggcg
     3241 gcggcaaggt gtggaccaac cgcggcaaga cgccctggga ggtcggaggc ttcatgctcc
     3301 cgcgctacgg tttcctggcg aaagcggggc agacgatggc cgcgatcttc gcgcgcgagg
     3361 ggctttcggt cgctctggcg cgcagcccgg gtgttacctt cgtggacgcg cgcccgctgt
     3421 cgctcgatgt cgccagccgt gctccggtga agacgcgcgt gctgggcgca cgccacctgg
     3481 gaggcggcca ggtggaggtg gagtttgaat gggaggtgtt gcgccccctg ccggaggagt
     3541 accgaccgtt cgttcacgtc tcgcatccca aggcggtggc tcaggggagc gaccggatcg
     3601 cgattcacgc gccgagcggg ctggagcccg agaagctgca gcaggtaggc acacaccgat
     3661 gcacaatccg gttcaccttc gccagcgcgc cgtatgacgg cgattactac ctgcgctacg
     3721 ggatctactc gcccaagcgg gggaaccggc tcttgcctct cgccgatatc gacggcaccc
     3781 gggtgcgcgg cggcatcttc acggtgaagc gcgtccaggg cgcgattgag tcggtgaagt
     3841 acgccccaga acggcctggc cccgacgccc caaaggagaa cgcggaaggg aagaagatcg
     3901 atttcgggtc ggtcgtcacg aacggcgcgt ttcgcctgct ccactctggg aatgagtggc
     3961 ggctctaccc gctccagggg agcttcccct tccaggcgga tctgcgcctg gccacgctgg
     4021 gcgccaaagg gaagcgcgtc acctcggtgg acggcctcga ccagtccggc aagcctctcg
     4081 gcgctgtgac tttcgagcag aagggagacg aggtgcatct cgacctggac gcggcggcgt
     4141 tctcctaccg gatcgtgttg aggtagcgag tgatggccga accggctggg gatggagatc
     4201 ggatggagag cctgcaagcc gccgcgcggg ggcgcatgct ccgcgcggga cagcggccag
     4261 cgctcccgcc gccggaaggc gtggagctgc gtcggagcgt ggtgtatggc cgggccggtg
     4321 gt
//
LOCUS       DAOSCV010000313         4319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12824_length_4319_cov_2.878987, whole genome shotgun sequence.
ACCESSION   DAOSCV010000313 DAOSCV010000000
VERSION     DAOSCV010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_4319_cov_2.878987"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..1991
                     /locus_tag="PLY56_08745"
     CDS             297..1991
                     /locus_tag="PLY56_08745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ21608.1"
                     /translation="MAQLDQRPEIPGKTILRVMVVACLVGIIVVVDLALLPQVEGAFI
                     YYLAIVLATNWFGGWGILLVPISLGLYHWNVAVTLPPGVRYPYLEDLTSLISFSLIAA
                     LTLQARERYHRVLAAEREQERLREAAEARGTRLEALYRVASAANVSRDPEEFLSLILD
                     EVRRLIPHTAAATVLERVDSHLVRRAASGPLPSVAIGEKIPLGHGIAGRVAAYGEPVI
                     LEGAAFQQAFIESPYLRALHPGAAAGVPLQIKGEETGSIVLFGDRDAHFSADDIRVLQ
                     SIASEAALVLREMHLLASLTRTNEELQERQRILDEQLDLARDVQKAIISVCPEQCTLG
                     PFQTAAYHASALQVGGDLLRIIPQEEGAALFVGDVMGKGVPAALLMTMMTAELSSPTV
                     SADRLEVILRDANHALAHNIASLDASGFVTVFYAFLSKEGPCVRYASAGHPPGLLVHA
                     DGSMEALAAQSLPLGVFADTEYEARERRLAPGDRLLLYTDGLIEARDGSGGFYGCERL
                     ERLLAEARQASALDLRDRIVADVNAFMKGNPPADDIAFLVVAVQEEAPQSSEPASS"
     gene            complement(1897..2973)
                     /locus_tag="PLY56_08750"
     CDS             complement(1897..2973)
                     /locus_tag="PLY56_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ21609.1"
                     /translation="MEAGLEIGDALTARLLSRVQRAFPLDPRPFAALGEALGLSEEQL
                     LSRISELKAQRVIRQISGIFDSRRLGYQSCLVAAQVPPSILEQAATAINRHPGVSHNY
                     ERAHAFNLWFTLTVPGQADLPAEATRLGMEAGAIQVRLFPTVRTFKIGVILDVGGDDG
                     GHPALPATAASPSGTADSAGARRLSAREIAAVRALQNDLPLVAHPFLALAKEAGMAEE
                     ELLAHARQFIEGGEMRRFAAVLFHRRAGFTANGMVVWNVPAERVVAVGQQFAGYPAVS
                     HCYQRPCYPDWPYSLFTMIHARSQEECERHVREMAGAAGIHDHAILYSVREFKKTRVR
                     YFEEPLPEQPPRETRCRPQADSPS"
     gene            complement(3001..3870)
                     /gene="lipA"
                     /locus_tag="PLY56_08755"
     CDS             complement(3001..3870)
                     /gene="lipA"
                     /locus_tag="PLY56_08755"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOJ21610.1"
                     /translation="MAHSVRPEWLRVTAPGGVNYRDLKRLVSSHHLHTVCESARCPNI
                     GECWERRTATFMILGNTCTRNCAFCAVPTGRPTEYDTEEPERVAAAVVRLGLRHAVIT
                     SVTRDDLADGGASIFAATIQAIRRQAPRCSVEVLIPDFKGSREALQVVMDARPTILNH
                     NLETVASLQRSVRPQADYQRSLAVLRMAREMAPDALTKSGIMLGLGEEWDEILETMAD
                     LRSVECNILTLGQYLRPSLDHLPIRRYYTPEEFETLREKGLAMGFTHVEAAPLVRSSY
                     HAAEQAEAPLGGR"
     gene            complement(3886..>4319)
                     /gene="nadA"
                     /locus_tag="PLY56_08760"
     CDS             complement(3886..>4319)
                     /gene="nadA"
                     /locus_tag="PLY56_08760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOJ21611.1"
                     /translation="WKGFCSVHQRFTVEQIAAAREKYPDVRVIVHPECRHEVVQAADD
                     DGSTEYIIKRITEAPAGTTWAVGTEVNLVHRLAQQQPDKQIFCLDPVVCPCSTMYRIH
                     PMYLLWVLDHLLQGEVVNEIVVRPDIIAQARVALDRMLGFA"
BASE COUNT          726 a         1390 c         1456 g          747 t
ORIGIN      
        1 agccacgggt cgggcgatct gcctgtgcca ccctgttgag gcgcatcccg tcgttgcccg
       61 ccgcatccaa gcgggcgatc ccgggcagag gcagcgcggc catcagcatg aaggagatca
      121 ggcctgcgcg gccaccgggg aagcgtcttc ggctcaccat cgtcttcgag tcctccatcg
      181 ctcacttcaa cacacgatgg ggttctcctt cgggccccag caacccccgc tgtttgatgg
      241 cgcacaagcc agggtaaatc caggcgagag tgaccggggc acaacgagcc ggaggggtgg
      301 cccagttgga ccagaggcca gagataccag gcaaaacaat cctccgcgtc atggtggtag
      361 cgtgcctggt gggcatcatt gtcgtggtag acctggcgct gttgccacag gtggaagggg
      421 cgttcatcta ctacctggcg attgtgctgg caacgaactg gtttggcggg tggggcatac
      481 tcctcgttcc gatctcgctc ggcctatacc actggaacgt tgcggtcact cttcctcctg
      541 gggtacgcta cccctatctc gaggatctga cgagtctcat cagcttctcg ctcatcgcgg
      601 cgctcacgct gcaggcgcgg gaacggtatc accgcgtcct cgccgcggag cgcgagcagg
      661 agaggctgcg cgaggccgcc gaggcgcgag gcacgcgcct ggaggcgctc tatcgcgtcg
      721 ccagcgccgc caacgtctcg cgtgatccgg aggagttcct ctcgctcatc ctggacgagg
      781 tgcgccgtct gatcccgcac accgcggcgg ccacggtctt ggagcgcgtg gatagccacc
      841 tggttcgccg ggcggccagc gggccgttgc cctcggtggc gatcggagag aagatccctc
      901 tcgggcacgg catcgcgggg cgcgtcgccg cttatggaga gccggtgatt ctcgaaggcg
      961 ccgcgtttca gcaggcgttc atcgagagtc cctatctgcg cgctctccac cccggcgccg
     1021 ctgccggagt gcccctgcag atcaaagggg aggagaccgg aagtattgtc ctctttggcg
     1081 accgcgacgc gcacttctcg gccgatgaca ttcgtgtgct ccaatcgatc gcctccgaag
     1141 ccgcgctggt gttgcgggag atgcacctgc tcgccagcct gacccgcacc aacgaggagc
     1201 tgcaggaacg ccagcgcatc ctggatgagc agctcgatct ggcgcgcgac gttcagaagg
     1261 cgatcatctc cgtttgcccg gagcagtgta ctctcgggcc tttccagacc gcggcatatc
     1321 atgcttcagc tctgcaggtg ggcggggatc tcctccggat catcccccag gaggaagggg
     1381 ccgccctctt cgtgggcgat gtgatgggga agggcgtgcc ggctgccctg ctgatgacga
     1441 tgatgaccgc ggaactaagc agccccactg tctccgcgga tcgcctcgag gtgatcctcc
     1501 gggatgcgaa tcacgcgctc gcccacaaca tcgcgtcgct cgatgcatcc gggtttgtca
     1561 cggtcttcta cgcctttctc tccaaagagg ggccgtgcgt ccgctacgcg agtgccgggc
     1621 acccgccggg attgctggtc catgcggatg gctccatgga ggcattggcg gcgcagagcc
     1681 tccccctggg ggtcttcgcc gatacggagt atgaggcgag ggagcgccgc ctggcgccgg
     1741 gagatcgcct tctcctctac accgacggat tgatcgaggc acgcgacggc tccggcggat
     1801 tctacggctg cgagcgtctg gaacgcctcc tcgccgaggc acgccaggcg tcggcgctcg
     1861 acctgcgtga ccgaatcgtg gccgacgtga acgcgttcat gaaggggaat ccgcctgcgg
     1921 acgacatcgc gtttctcgtg gtggctgttc aggaagaggc tcctcaaagt agcgaacccg
     1981 cgtcttcttg aactcgcgga cgctgtaaag gatcgcgtga tcgtggatac cggccgcgcc
     2041 ggccatctcg cgcacgtggc gctcgcactc ttcctggctc ctcgcgtgaa tcatggtgaa
     2101 gagggaatag ggccagtccg ggtagcaggg gcgctggtag cagtggctca ctgcgggata
     2161 gccagcaaac tgttgaccca cggcgactac gcgctcggcc ggcacgttcc agaccaccat
     2221 tccgttggcg gtaaagccgg cgcgccggtg gaagaggacc gcggcgaagc ggcgcatctc
     2281 gccgccctcg atgaactgcc gcgcgtgagc gagtagctcc tcctccgcca tgccggcctc
     2341 tttagccagc gcgaggaaag ggtgcgccac cagcggcaga tcgttctgga gggcgcgcac
     2401 cgcggcgatc tcccgcgcgc tcaacctcct ggcgccggcg gaatcagcag tgcccgatgg
     2461 ggaagcggcg gtcgcgggca gggccggatg gcctccgtcg tcgccgccaa catccaggat
     2521 gaccccaatc ttgaaggtgc gcaccgtggg gaagaggcgg acctggatgg cgccggcttc
     2581 cataccgagc cgggtcgctt ccgcggggag atcggcctgc ccagggacag tgagcgtgaa
     2641 ccacaggttg aaggcgtggg cgcgttcgta gttatggctc accccaggat ggcggttgat
     2701 cgccgtggct gcttgctcca ggatgcttgg tggcacctgg gcggcgacaa gacagctctg
     2761 atagccgagg cggcgggagt cgaagatacc gctgatctgg cggatgacgc gctgtgcctt
     2821 gagctcgctg atccgggaga ggagttgctc ctcggaaagg cccagggcct cgcccagcgc
     2881 cgcgaaaggg cggggatcga gcgggaacgc gcgctgcacc cgcgagagga ggcgggcagt
     2941 gagggcatca ccgatctcca gtccagcctc catgcgtctc tctccgaaaa agtggccggg
     3001 ctatctgcct cccaggggag cctcagcctg ctccgcggca tgataggagc tgcgcaccag
     3061 tggcgccgcc tctacgtgcg tgaagcccat ggcgagacct ttctcgcgaa gcgtctcgaa
     3121 ctcctccggc gtgtagtagc gccggatggg gaggtgatcc agcgagggcc gcaggtactg
     3181 cccgagcgtc aggatattgc actccaccga gcgcaggtcg gccatcgtct ccaggatctc
     3241 gtcccactcc tcgcccaggc caagcatgat ccccgatttg gtgagcgcat ccggcgccat
     3301 ctcgcgggcc atgcgcagga cggcgagcga ccgctggtaa tccgcctggg ggcgcactga
     3361 acgctgcagc gaggcaaccg tctccaggtt gtggttgagg atggtcgggc gcgcatccat
     3421 caccacctgc agcgcctcgc ggctgccctt gaagtctggg atcagcacct cgacgctgca
     3481 tcgcggggcc tgccggcgga tcgcttgaat ggtggccgcg aagatcgatg cgccgccatc
     3541 ggcgaggtca tcgcgggtga ccgaggtgat cacggcatgg cgcaggccaa ggcggacaac
     3601 ggcggcggcg acgcgctccg gttcctcggt gtcgtactcc gtgggccggc ctgtcggcac
     3661 cgcgcagaag gcgcagttgc gggtgcatgt gtttccgagg atcatgaagg tcgccgtgcg
     3721 ccgctcccaa cactcgccga tattcgggca gcgagcgctc tcgcagaccg tgtggagatg
     3781 atggcttgaa accaggcgct tcagatcccg gtaattcacg ccaccgggtg ccgtgacccg
     3841 cagccactcc gggcggaccg agtgtgccaa accaaccccc tttgactacg caaagcccag
     3901 catgcggtcg agtgccacgc gcgcctgggc aatgatatcg ggccgcacca caatctcgtt
     3961 gaccacttcg ccctggagca ggtggtcgag aacccacagc agatacatcg ggtgaatccg
     4021 gtacatcgtc gagcaggggc agaccaccgg atcgagacag aagatctgct tgtccggctg
     4081 ctgctgcgcc aggcggtgga ccaggttcac ttcggtgccg acggcccagg tggtgccagc
     4141 gggcgcctcg gtgatgcgct tgatgatgta ctcggtggag ccatcatcat ccgcggcttg
     4201 aacgacttca tggcggcatt ccgggtgcac gatcacgcgc acatcgggat acttctcgcg
     4261 cgccgcggcg atctgctcga cggtgaagcg ctggtgtacc gagcagaaac ctttccaca
//
LOCUS       DAOSCV010000314         4318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12829_length_4318_cov_3.084682, whole genome shotgun sequence.
ACCESSION   DAOSCV010000314 DAOSCV010000000
VERSION     DAOSCV010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_4318_cov_3.084682"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLY56_08765"
     CDS             complement(<1..571)
                     /locus_tag="PLY56_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ21612.1"
                     /translation="MGRIYLDYAATTPVDPQVVEAMRPYWGDVFGNPSSAHAFGREAR
                     AAVDTARDTVARCLGARSDEIFFTSGGTESINLAIRGVVEASEHPSPHLITSAIEHHA
                     VLDTCRYLERRGAQLTVLPVDANGLVDPEAVRGAITPQTVLVSIMLANNEIGTLQPIA
                     EIARICREAGVLIHTDAVQAVGHLRLSFAD"
     gene            complement(589..1533)
                     /gene="cysK"
                     /locus_tag="PLY56_08770"
     CDS             complement(589..1533)
                     /gene="cysK"
                     /locus_tag="PLY56_08770"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ21613.1"
                     /translation="MKIASDITRLAGGTPLVRLNRVVEGAEATVVAKLEMFNPCSSIK
                     DRIGVAMIDAAEAAGLINKETVLIEPTSGNTGIALASVCAARGYKLVLVMPETMSQER
                     RSLLKMLGAQLVLTPGAEGMPGAVRRAEELVAEAPNRWMPQQFRNSANPDIHRRTTAE
                     EIWRDTDGQVDIVVGGVGTGGTLTGIGEALKPRKPGLRIVAVEPAESPMITEGRRGPH
                     PIQGIGAGFIPDVLRLDILDETITVNGSDALQMARRLAREEGILVGISSGAAAWAAVQ
                     VAKRPENAGRMIVVILPDTGERYLSTQLFQDLPERSYI"
     gene            complement(1825..2082)
                     /locus_tag="PLY56_08775"
     CDS             complement(1825..2082)
                     /locus_tag="PLY56_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21614.1"
                     /translation="MNSDREQAKGRIERTFTGDAPTGENVVDTTGPEAVPGMDVGASE
                     LPTRGDLGFPGSPNEELEELCTGPIDVGAMRPRDMEEDSPR"
     gene            complement(2459..>4318)
                     /locus_tag="PLY56_08780"
     CDS             complement(2459..>4318)
                     /locus_tag="PLY56_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOJ21615.1"
                     /translation="KAVPRKSPFIDADLPLGKPVGKSKGKKEAEAPAEEPAVAVLPEP
                     KPEPEPEAEPEPEPIQPVLWNARSKKEPEPAVEEAPAEEPPAPKKPAKAKPAEAPAES
                     AEQVEEKAEEEGDPAATQREYTLPPTTLLQVAQVVPDGPGAVSDTARNIKILEETLAQ
                     FKIEAKVVEIAQGPTFTRYEIRLAPGIRVNRIVSLGDNIAMNLAALDVRIQAPIPGKA
                     AIGIEVPNKSMAMVPLRDVVESPEFQNATSKLTFALGKDVTGRSWVADLARMPHLLIG
                     GATNSGKSVCLNGLIASILFRVKPEEVKFLLVDPKRVELSLFDGIPHLLYPVITDIKK
                     AAGMLNWAVREMDRRYELLEKNGCRDIYSYQARAAEEGLEQLPFIVIVVDELADLMLQ
                     LAAEVEQTICRLAQLARATGIHLVIATQRPSVDVITGLIKANIPSRIAFAVSSHIDSR
                     TILDKRGAERLLGRGDMLFEPIGAAKPVRIQGAYVSEREIETLVEYLRRQATPVYTAE
                     PVEAPNAGGNGHAGGGDADGAEDSLFEDAVRLVVTTRRASTSMLQRKFSIGYTRAARL
                     IDMMEERGIVSALDGAKPREVLVSREQIEQFFRSPLNAPPEEPAGSEDENPFV"
BASE COUNT          670 a         1422 c         1406 g          820 t
ORIGIN      
        1 tgtccgcgaa ggaaagccgg aggtggccga cagcctgcac ggcatcggtg tggatgagga
       61 cgcccgcctc ccgacagatg cgggcaatct ccgcgatcgg ctgcagcgtg ccgatctcat
      121 tgttggcgag catgatggag accaggacgg tctgcggggt gattgcgcca cggacggcct
      181 ctggatccac caatccgttt gcgtccacgg ggagcacggt gagctgcgcg ccccgcctct
      241 ccagatagcg gcaggtatcg agcacggcat ggtgctcgat ggccgaggtg attagatggg
      301 gcgaggggtg ctcactggcc tccaccacgc cccggatggc caggttgatc gactcggtgc
      361 caccgctggt aaagaagatc tcatccgagc gtgcgcccag gcatcgggcg accgtgtcac
      421 gcgcggtatc caccgcggcg cgggcctcgc gcccgaaagc gtgcgcgctc gatggattgc
      481 cgaagacgtc gccccaatag gggcgcatcg cctcaacaac ctgcggatcc accggggttg
      541 tggcagcgta gtcgagataa atccggccca tcagatatag aacccctgct agatatagga
      601 cctctccgga agatcttgga agagttgggt ggagaggtag cgctcgcccg tatcggggag
      661 gatcaccacg atcatgcgcc cggcgttttc cggccgcttg gctacctgca cggccgccca
      721 cgcagcggcc ccggaggaga tcccgacgag gatgccctcc tcgcgggcaa ggcggcgggc
      781 catttgcagc gcgtccgagc cattcaccgt gattgtctca tcgaggatgt ccagacggag
      841 cacgtctggg atgaagccag cgccgatgcc ctggatgggg tgaggaccgc gtcgcccttc
      901 ggtgatcatc ggggactcgg ccggctcgac cgccacgatt cgcagcccgg gcttgcgtgg
      961 cttgagcgcc tcgccaatgc ccgtgagggt acctccggtg ccaaccccgc cgacaacgat
     1021 atccacctgg ccatccgtat cgcgccagat ctcctcggcg gtggtgcggc ggtggatatc
     1081 cgggttggca gagtttcgga actgctgggg catccagcgg ttgggggctt ccgccaccaa
     1141 ctcctcggcc cggcgcacgg cccctggcat tccctcggct cccggcgtga gtacgagctg
     1201 cgcaccgagc atcttcagga gtgagcgacg ctcctggctc atcgtctccg gcatgacgag
     1261 caccagctta tagccacgag cggcgcagac agacgccagt gcgatccctg tgttgccgga
     1321 ggtcggttcg atcagaaccg tctccttgtt gatcaaccct gctgcttccg ccgcatcgat
     1381 catggccact ccgatgcgat ccttgatgct ggagcaggga ttgaacatct ccaacttcgc
     1441 gacgaccgtg gcctcggccc cttccacgac tcggttcagc cggacgagcg gtgtcccgcc
     1501 tgctaaccgt gtgatgtcac tggcgatctt cacctttcca ctccttcagg cgtcactata
     1561 acaccagtcc tgaacgaatg tcaaggtgat cctcaggcgt ttggagtcac agcgcgggct
     1621 gtgcaggggc gcgcacaaac acccgcgctg cttgtgggca aagccctgcc gggccgggtc
     1681 acgatgggca cttgccgatg ggtatgccct tgtcacccat acgtgtccgc cccctccatc
     1741 cacggcatcg gtgtcccggg atgggcggtc gtcgggacgg tttacctcat gattccggca
     1801 ccctgggtcg tgcggtcttc gggctcagcg cgggctgtcc tcctccatgt cgcgcgggcg
     1861 catggcgcca acatcgatgg ggccggtgca cagctcctcc agctcttcgt tgggcgatcc
     1921 ggggaagccg agatcgccac gggtgggcag ttcgctggcg ccgacatcca ttccggggac
     1981 ggcttctggg cccgtcgtgt ccaccacgtt ctcgccggtg ggcgcatcac ccgtgaatgt
     2041 gcgttcaatg cgcccctttg cctgctctcg atccgaattc atagcgctct ctccctcgaa
     2101 gccggcgtgc cgatgcggcc atctaggtgc ttgttccggc gcgctgcccc gccttcagct
     2161 tcatcctctg gcgtggcgct tctggccgga cgactccaaa gagttgtttc gcgtccggct
     2221 ttccccggct ggcgtcctgc caaacccagg gagatagtgg gagaggagtg gggaagggag
     2281 gtcgtgtcag gaaggatgga tgaaccgcgg tagctcacgc actcctgaaa tcgctgcagg
     2341 agaatgcgcc gcctgcccct cctggcacgg tgggccgggg gtagggcttc cccgagagga
     2401 gcgcatctag ctcagcctcg cgcccaggag agtctgcccc cgggcgcgct ctctcttgtc
     2461 agacgaaggg gttttcatcc tcggaaccgg cgggctcctc gggaggcgcg ttcagcggcg
     2521 agcggaagaa ctgctcgatc tgctcgcggc tgacaagcac ctcgcgcggc ttggcaccat
     2581 ccagggcgct gacgatcccg cgctcctcca tcatatcgat gaggcgcgcc gcgcgcgtgt
     2641 agccgatgga gaacttccgc tgcaacatcg aggtggacgc ccggcgggtg gtcaccacga
     2701 ggcgaaccgc gtcctcgaag aggctgtctt cggccccatc cgcgtctccg cccccggcgt
     2761 ggccgttgcc gccggcgttc ggggcttcca ccggctccgc ggtgtagacc ggggttgcct
     2821 ggcggcggag atactcgacg agtgtctcga tctcgcgttc gctgacgtaa gccccctgaa
     2881 tgcgcaccgg cttcgcggct ccgatcggct cgaagagcat gtcgccccgg cccaggagac
     2941 gctcggcgcc gcgcttatcg aggatggtgc ggctatcgat gtgcgaggag acggcaaagg
     3001 cgatacgcga ggggatgttc gctttgatca ggccggtgat tacatcgaca gagggccgct
     3061 gcgtggcgat caccaggtgg atgccggtcg cgcgcgcgag ctgcgccagg cggcagatcg
     3121 tctgctccac ctcggcggcg agctgcagca tcagatccgc cagctcatcg accacgatga
     3181 cgatgaaggg aagctgttcc agaccttcct ctgcggcgcg tgcctggtag gaatagatat
     3241 cgcggcagcc gttcttctcc agcagctcgt agcggcggtc catctcgcgc accgcccagt
     3301 tgagcatgcc cgctgccttc ttgatgtcgg tgatcaccgg gtagagcagg tgtgggatac
     3361 catcgaagag gctaagctcc acgcgctttg ggtcgaccag gaggaacttc acctcctccg
     3421 gcttcacccg gaagagaata ctcgcgatga ggccgttgag gcagacgctc tttcccgagt
     3481 tcgtcgctcc cccgatcagc aggtgcggca tgcgcgccag gtcggccacc cacgagcgcc
     3541 cggtgacgtc cttgccgagg gcgaaggtga gtttggacgt ggcgttctga aactcgggcg
     3601 attccaccac atcgcgcagg ggcaccatgg ccatggactt gttcggcacc tcgatgccga
     3661 tagccgcctt gcccggaatc ggcgcctgga tccgcacatc cagcgccgcc aggttcatgg
     3721 cgatgttatc gccgagcgac acgatccggt tcacgcgaat cccgggggca agccggatct
     3781 cgtagcgggt gaaggtgggg ccctgggcaa tctccactac cttggcctcg atcttgaact
     3841 gggcgagcgt ctcttcgagg atcttgatgt tgcgcgccgt atcgctcacc gctccggggc
     3901 catcaggcac cacctgcgcc acctgcagca gcgtggtggg cggtagcgta tactcccgct
     3961 gggtggccgc cggatcgcct tcctcctcgg ccttttcctc cacctgttcc gctgattcgg
     4021 ccggcgcctc tgccggtttc gcctttgccg gcttcttcgg cgcggggggc tcctcggctg
     4081 gcgcctcctc gaccgctggc tccggttcct tcttgctcct cgcgttccag agaaccggct
     4141 ggatcggctc cggctcgggt tccgcctccg gctcgggttc aggttttggt tcggggagaa
     4201 ccgcgaccgc cggctcctcg gcaggtgcct cggcttcctt cttgcccttg ctcttgccaa
     4261 ctggcttgcc caagggaaga tccgcatcaa tgaagggcga cttgcgaggc actgcctt
//
LOCUS       DAOSCV010000315         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12894_length_4300_cov_3.628975, whole genome shotgun sequence.
ACCESSION   DAOSCV010000315 DAOSCV010000000
VERSION     DAOSCV010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12894_length_4300_cov_3.628975"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..546)
                     /locus_tag="PLY56_08785"
     CDS             complement(247..546)
                     /locus_tag="PLY56_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOJ21616.1"
                     /translation="MPVDVPERVTERPLPTERVPGTGDGDHVVIVFNNDYNTYDEVIA
                     ILQAATGCSREEAEMETWEIDHLGRSLVHYGQREECERAAAIIRTIGIRVSVSEL"
     gene            complement(738..935)
                     /locus_tag="PLY56_08790"
     CDS             complement(738..935)
                     /locus_tag="PLY56_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21617.1"
                     /translation="MRNTLYLLLAAAGLVGLAALLARRGRPSRRDPTREVERLLDRCR
                     ELLDGIESAARSLDSSEPSPS"
     gene            complement(1048..1464)
                     /locus_tag="PLY56_08795"
     CDS             complement(1048..1464)
                     /locus_tag="PLY56_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21618.1"
                     /translation="MAKRILVVDDERHIVRLVQVNLERVGYEVVTAFDGKEAVGKVKE
                     VNPDLIVCDVMMPYMDGFEVLRTIRADPETRDIPFIMLTAKAQDADIFRGWSSGVDAY
                     LTKPFNPMELLAFVKRIFQSLSEPGEKRFDLGGDGS"
     gene            complement(1643..2068)
                     /locus_tag="PLY56_08800"
     CDS             complement(1643..2068)
                     /locus_tag="PLY56_08800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21619.1"
                     /translation="MGSAETVFVEFTAAPEYVAVARRVAEVIGARAGLSPVRLEELQI
                     AVSEVCNRLVNAAQADPGRAHSVSLRFRIDAGAVVAEVGARSTALEQTLRLLLDEEFP
                     LYLMRRLVDRVERFQSQEGPGLRLIKGRSPRGEQNGSLP"
     gene            complement(2068..3624)
                     /locus_tag="PLY56_08805"
     CDS             complement(2068..3624)
                     /locus_tag="PLY56_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HOJ21620.1"
                     /translation="MAEQRGGAVRPPEGDDRLSALLTNANAVRAVASAVAGTLGPKGL
                     DCMLLNSGGDVIITNDGSTILDQIAVSHPAAHLLINAARAQEREVGDGTTTTTLLANA
                     LIDAAVHHVTRGVPVVRLLEGMRVAMARAQSFLDEIAVACPDLDDPFLCRAALVAGRG
                     YPEIADLVVRAARLVGREMLSDPSFRLADRVVAQDGLESAVLEGVVIRKGRMNRQMPV
                     QLAEARVLIIDDALEPEALGEEALATEAGFRRFLQVQEEFKAGLRMLMESGVRCVLVE
                     RSVDPMAEEALTEAGVLVVRRVAPSDLASVAELCGARPVKRTVLKRRPEEVSAALGYA
                     DKVVVDDRRQFLCITGGRGQRVATILVGASTREVRDERERIARDAASAVQAVAVGGVV
                     PGGGAAELAAARAVQALRAEMRGMAAYGADCAIEALRVPLMQIVSNAGFNPLEKVEDV
                     LAAQAARESPALAIDCDTGEVTDMMRAGVVDPLPVKKQALRTAFEVAEAILRIDRIIK
                     KRQEPEAAGE"
     gene            complement(3636..>4300)
                     /locus_tag="PLY56_08810"
     CDS             complement(3636..>4300)
                     /locus_tag="PLY56_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HOJ21621.1"
                     /translation="PPALEEDVVRQVHLAVIDRERLLVVIALGTGRVEHAVVSPGVDL
                     MSVDLGRIEHQVGTAIRGHTVAQALDAVPDPVEGYLPDAQKVYHAIMHAAADVLKRIG
                     TADAGEVVVEGTAAVLRQPEFGHTEVIRELVAFLEERRQLFSLLRDVYDEESRIIIGS
                     EHSDRRMQMCSLVLARYTVLGRYAGTIGVLGPTRMDYDRTLPVVRFMARSVGELLTQL
                     RL"
BASE COUNT          763 a         1527 c         1330 g          680 t
ORIGIN      
        1 agctccggca ggagaagtac gccacagagg cctggaacct gcgccggtag ccggtcgcct
       61 gcctccaccg gcgcgttgca ggccgggcta aacaggccct ctcgccaccg cccatcgtag
      121 gcccccccag tatctctgcg cggtgcccat caggagacgc gggatcgcgc tacggttttc
      181 ctgggggcgg aagatcggaa ggggcgcgcc agcgacggca gagcgaccgg aaagcagtgg
      241 gagcggttag agctcgctca cggagacgcg gatgccaatg gtgcgaatga tggcagcggc
      301 gcgctcgcac tcttcgcgct ggccgtagtg gacaagggag cggccgagat ggtcaatctc
      361 ccatgtctcc atctccgcct cttcgcgaga acagccggtg gccgcctgga gaatggcgat
      421 cacctcatcg taggtgttgt agtcgttgtt gaagacgatc accacgtggt cgccgtcgcc
      481 cgttccgggc acgcgctccg tgggtagtgg gcgctccgtc acccgctcgg gtacgtctac
      541 aggcatcgtc tctcctccaa gcccagactg acaccgtgat tatacccccc cccttgcaag
      601 aaataacgcg aaggaggagg aaacccgggc atccgagagg cacaccgcga gcgcgccggc
      661 agcacccgca gcgaggaaga catcgcctgt ttgcctgccg gcgcatcgat gcccaccgga
      721 tgcaccgagg cacgcgctca ggaaggggag ggctcggagc tatccagact gcgtgcggcg
      781 ctctcgatgc cgtcgagcaa ctcgcgacag cggtcgagca agcgctcgac ctcgcgtgtc
      841 ggatcgcgac gcgacggccg cccgcgacgg gccaggaggg cagcaagccc caccaggccg
      901 gctgccgcca gcagtagata aagggtattc cgcatagaag caggctctcg gtaatgcagc
      961 atcgattccc ggagcatcag gccaggcaaa cggtttgagg ggcctgacgg ggaatcgccc
     1021 gaatgtatcg ggtcacagag gcgccagcta gctgccgtcg ccgcccagat cgaagcgctt
     1081 ctcgcctggc tcgctcagcg actggaagat gcgcttgacg aaagcgagga gctccatcgg
     1141 gttgaatggc ttggtcagat acgcgtcgac gccggaggac catccccgga agatatcggc
     1201 atcctgggcc ttcgccgtca gcatgatgaa ggggatatcc cgggtctcgg ggtcggcccg
     1261 gatggtgcgc agaacttcga agccgtccat atacggcatc atgacgtcgc acacgatcag
     1321 gtccgggttc acctctttga ccttgccaac cgcttcctta ccgtcgaagg cggtcaccac
     1381 ctcgtagccg acacgttcga gattcacctg gaccaggcga acgatatgcc gctcatcatc
     1441 cactaccaga attcgctttg ccatgagcgt ctaacgcctc ctcagctttc tatatgtacg
     1501 gcgccggact tgggagaatg ggctgggctg ctgttccggc gaggtggcag caaccgccac
     1561 aaggcctgcg gtatcggtcc attgtgggcg ctagcgatcg actcgctacc gtggccatta
     1621 tacacgaacg ccagaatcgt tgtcaaggca acgaaccgtt ctgttctccc cgggggcttc
     1681 gccccttgat caggcgcagg cccggccctt cctgcgattg gaatcgctcc acgcggtcca
     1741 caagccggcg catcaggtag agcgggaact cctcgtccag caagagccgc agcgtctgct
     1801 cgagggcagt actgcgggcg cccacttcag ccaccacggc gccggcatcg attcggaagc
     1861 gcaggctgac cgagtgggct ctgcccgggt cggcctgagc agcgttgacc agccggttgc
     1921 acacttcgga gacggcgatc tggagttcct ccaggcgaac gggcgaaagg cccgcgcgcg
     1981 cgccgatcac ctccgccacc cggcgggcaa cggccacgta ttcgggcgcc gccgtgaact
     2041 ccacaaagac cgtctcagcg cttcccatca ctcccccgcc gcctccggct cttgccgctt
     2101 cttgatgatc cggtcgatgc gcagaatcgc ctcggccacc tcgaacgccg tccgaagcgc
     2161 ctgtttcttc accggaagcg ggtccactac gcccgcgcgc atcatgtcgg tcacctcgcc
     2221 ggtatcacag tcgatcgcga gtgccggcga ttcacgcgcc gcctgagccg cgagaacatc
     2281 ttccaccttt tcgagagggt tgaagcccgc gttggacacg atctgcatca gcggcacccg
     2341 cagcgcctcg atggcgcaat cggcgccgta ggccgccatc ccgcgcatct ccgcgcgcag
     2401 ggcttgcacg gcgcgcgcgg ctgccagttc ggccgcgcct ccgcccggca caaccccacc
     2461 cacggcaacc gcctggacgg cggaagcggc atcccgcgcg atccgctcgc gctcgtcgcg
     2521 cacctcgcgc gtagacgcgc ccaccaggat cgttgccacc cgctggcccc tgcccccggt
     2581 gatgcaaaga aactggcggc ggtcgtccac caccaccttg tcggcgtatc ccaacgccgc
     2641 agaaacctct tccgggcggc gcttgagcac ggtgcgcttc accgggcgcg ccccacacag
     2701 ttctgccacg ctcgccaggt cgctcggcgc aacccgacgc accaccaaca cgccggcctc
     2761 ggtcagtgct tcctcggcca tgggatcgac ggagcgctcg accagcacgc agcgaacgcc
     2821 ggactccatc aacattcgca ggccggcttt gaactcctcc tgaacctgca agaaccgccg
     2881 gaaacccgcc tcggtcgcca gcgcctcttc gccgagggcc tctggctcca gggcatcatc
     2941 gatgatgagc acccgtgcct cggcgagttg caccggcatc tggcggttca tccgcccctt
     3001 gcggattacc acgccctcga ggaccgcgct ctcgagccca tcctgggcta cgacacgatc
     3061 cgccagccgg aaggatgggt cgctcagcat ctcccggcca accaggcgcg cagcgcgcac
     3121 caccaggtcg gcgatctccg ggtagccccg gccggccacg agcgccgccc ggcacaggaa
     3181 cgggtcatcc agatccgggc aagctacggc gatctcgtcc aaaaagctct gcgcacgcgc
     3241 catcgccacg cgcatcccct ccaggaggcg cacaaccgga accccccgag tgacgtggtg
     3301 aaccgccgcg tcgatgagcg cgttggccag gagagtggtg gtcgtcgtgc cgtcgcctac
     3361 ctcgcgctcc tgagcccggg ccgcattaat gagcaggtgc gccgctgggt gcgagaccgc
     3421 gatctggtcc aggatggtgc tgccatcatt ggtgatgatg acatcccccc cgctgttcaa
     3481 cagcatgcag tcgaggcctt tgggccccag cgtgcccgcg acggcagacg cgaccgcccg
     3541 cacggcattt gcgttcgtca agagcgccga aagccgatcg tcaccttcgg gcgggcgcac
     3601 tgcccctcct cgctgttctg ccatcgctct cctaattaga gccggagttg ggtgagcaat
     3661 tcgccgaccg agcgagccat aaaccgcaca acggggagcg tccggtcata gtccatgcgc
     3721 gttgggccga ggacgccgat ggtgcccgca taccgcccga gcacggtgta gcgcgccaac
     3781 acaagcgagc acatctgcat gcgccgatcc gagtgctcgc tgccgatgat gatgcgcgac
     3841 tcctcgtcgt agacgtcacg cagcagcgag aagagctggc gacgctcctc cagaaaagcc
     3901 accagctcgc gaatcacctc ggtgtgcccg aactccggct ggcgcagcac cgccgccgtg
     3961 ccctcgacca cgacttcgcc cgcatccgcg gtgccgatcc gcttcaggac atcggcggcc
     4021 gcgtgcatga tggcgtgata caccttctgc gcgtccggca gatagccctc gaccgggtcg
     4081 ggcacggcat cgagcgcctg ggctaccgtg tgcccgcgga tggcggtgcc cacctggtgt
     4141 tcgatccgcc ccagatcgac gctcatcagg tccacgcccg gcgacaccac ggcgtgctcg
     4201 acgcgccccg ttcccaaggc gatcacaacc aagaggcgtt ctcggtcgat caccgccaga
     4261 tgtacctggc gaaccacgtc ctcctcgagc gccggggggg
//
LOCUS       DAOSCV010000316         4297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12909_length_4297_cov_3.501650, whole genome shotgun sequence.
ACCESSION   DAOSCV010000316 DAOSCV010000000
VERSION     DAOSCV010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12909_length_4297_cov_3.501650"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLY56_08815"
     CDS             <1..820
                     /locus_tag="PLY56_08815"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOJ21622.1"
                     /translation="EIIHYLGIAYLEPGDEVVQAHPSFVRYEAAAILNGCQCIQVPLK
                     DYTHDLDAMADAVTPRTKLLFITNPNNPTGTIVTRSAVDRLLDRIPERVLVVMDEAYY
                     EYVEDPEYPDSLAYIREGRNVISLRTFSKIFALAGLRVGYGIARPEIIWAIHQVREPF
                     NVNSIAQVGAIACLDDPDQVERGRRVNRDGRTYLYAACERLGLPYVPSQANFVFIDVK
                     ADSRVVFQELLRRGVIVRTGDIFGHPTHLRVTVGTPEENARFVRALEEVLAALK"
     gene            991..2037
                     /gene="aroF"
                     /locus_tag="PLY56_08820"
     CDS             991..2037
                     /gene="aroF"
                     /locus_tag="PLY56_08820"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOJ21623.1"
                     /translation="MIVVLKKNATEADLHTLSEEIEKRGYQVHLSRGVERTLVGCIGA
                     PEKDKDLLADQFAAFPFVEDVVRILKPYKIVNREFQPERSQIRIGNVVVGGDALVIMA
                     GPCSVESREQLLETAHGAKAAGAHILRGGAFKPSTSPYSFHGLGEEGLKLLAEARAAT
                     GLPIITEVMDTRDVELVARYADCLQVGTRNMANFSLLRELGEVRKPVMLKRGMSATLE
                     EWLQAAEYIASRGNYEIILCERGIRTFEPYTRNTFDISAIPACRELSHLPIVADPSHA
                     TGKRTLVPAVAKAAIAAGADGVMVEVHSRPERALKDGPQALTLIEFAQLMRDLEPFAA
                     AAGRPLKQGEAETE"
     gene            2253..3116
                     /locus_tag="PLY56_08825"
     CDS             2253..3116
                     /locus_tag="PLY56_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HOJ21624.1"
                     /translation="MAYSSICIIGMGLLGGSLGMALKQRGLARRVVGVARRPEALERA
                     RELGSIDEGGLDATAAVAGADLTFLCVPVLAITRLLPLLAPHVRAGGVVTDVGSTKTA
                     IVDAGESVLPGRFLGGHPMAGSEQAGIEGADPLLFEGATWALTPGEASAPVEPLSELI
                     TGLGARPLLLPPEEHDRAVAATSHLPHATAAALARVVADALGEAEAAPRLAAGSYRDT
                     TRVAASSPELWRDVCLTNREPILAALGRMQGYLAELAEALRQDDVVAVERFFRQGREA
                     KARMESARRKG"
     gene            3122..>4297
                     /gene="aroA"
                     /locus_tag="PLY56_08830"
     CDS             3122..>4297
                     /gene="aroA"
                     /locus_tag="PLY56_08830"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ21625.1"
                     /translation="MASLRIPTAGPLQGAARVPGDKSISHRAVMLGALAEGVTEIEGF
                     LPGADCLSTIACFRALGIQIEQPDPTRVIVHGAGEAGLQEPEDLLYVGNSGTTLYLTL
                     GVLAGQAFTSVLTGDSSIRRRPVQRVGIPLRQMGATILARRDGTLPPVTIRGGALQPI
                     TYTLPSASAQVKSAVLLAGLFAEGVTTAISPAVSRDHTERMLRGFGADVRVEGMRASV
                     RGPARLRAQPVSVPGDISSAAFLLVAGCIVPGSEILVESVGINPTRSGIVDALQAMGA
                     RITVEREREVAGEPVADLRVCASELRGTSLAGAIIPRLIDEIPILAVAAACATGDTVI
                     RDAGALRIKETDRIATTAALLRAYGVAVEVLEDGMVVHGAGDPRALKGAEIDSEGDHR
                     "
BASE COUNT          668 a         1374 c         1497 g          758 t
ORIGIN      
        1 tgagattatc cattacctgg gcattgccta cctggagccg ggggacgaag tggtccaggc
       61 acacccctcg ttcgttcgct acgaggcggc ggcgatcctg aatgggtgcc agtgcatcca
      121 ggtcccgctg aaggactata cgcacgacct cgacgcgatg gccgacgcgg tgacgccgag
      181 gacgaaactc cttttcatca ccaacccgaa caacccgact ggcaccatcg tgacgcgtag
      241 cgccgtggat cgcctgctcg accgcatccc cgagcgcgtg ctcgtcgtga tggacgaggc
      301 ctactacgaa tacgtggagg atccggagta tcccgattct ctcgcgtaca tccgcgaggg
      361 acgcaacgtg atctcgctgc ggaccttctc gaagatcttc gcgctggctg gcttgcgcgt
      421 tggctatggc atcgctcgtc cggagatcat ctgggcgatt caccaggtgc gcgagccgtt
      481 caacgtcaac tcaattgccc aggtcggggc gattgcctgc ctggatgatc ctgatcaggt
      541 ggagcgcggc cgtcgcgtca atcgcgacgg ccgtacgtac ctctatgccg cctgcgagcg
      601 tttgggcctg ccctatgtgc cctcgcaggc gaacttcgtc tttatcgacg ttaaggcgga
      661 cagccgggtc gtcttccagg agctgctgcg ccgcggggtg atcgtgcgaa cgggcgatat
      721 cttcggccat cccacgcatc tccgcgtgac cgtcggcact cccgaggaga atgcccgttt
      781 cgttcgcgcc ctggaggaag tgcttgccgc gctgaagtag ccgcaagcca ggccaagggt
      841 cgggagaagg gcgcgtctgt ctctggactg agacccgccc ctgcctcgtc gttgaggcgt
      901 ggggcgggat gggcattcgg gggctcgccg cttctcccgt tcttctccag ccgcacactc
      961 caggtattcc ctgatcggga ggctggcaca gtgatcgtcg ttttgaagaa aaacgcaacg
     1021 gaagcggatc tgcacacgtt atcggaggag atcgaaaagc gtggttacca ggtgcacctg
     1081 tcgcgcgggg tcgagcgcac gctcgtcggc tgcatcggtg cgccggaaaa agataaggac
     1141 ctgttggcgg accagttcgc tgccttcccc ttcgtggagg acgtggtccg gatcctgaag
     1201 ccatataaga ttgtcaaccg cgagttccag ccagagcgct cgcagatacg catcgggaat
     1261 gtagtggtcg gcggtgatgc gctggtcatc atggcgggac cgtgctctgt ggagagccgg
     1321 gagcagcttc tagagacggc tcacggcgcg aaggccgcag gggcgcacat cttgcgcgga
     1381 ggcgcgttca agccgagcac gtcgccctac tccttccacg gccttgggga agaggggctg
     1441 aagcttctcg ccgaggcaag ggcagcgacg ggcctgccga tcatcacgga ggtcatggat
     1501 acgcgcgatg tcgagctggt cgcgcggtat gccgactgcc tgcaggtggg cacgcgcaac
     1561 atggccaact tctcgctgtt gcgcgagctc ggcgaggtgc gcaagcccgt gatgctgaag
     1621 cgcgggatgt cagcaacgct tgaggagtgg ctccaggctg cggagtacat cgcttcccga
     1681 ggcaactacg agatcatcct gtgcgagcgg ggcatccgca ccttcgagcc gtacacccgg
     1741 aacaccttcg atatcagcgc gattccggca tgccgggagc tctcgcacct gcccatcgtg
     1801 gcggatccga gccatgccac gggcaagcgc acgttggtgc cggctgtggc caaggccgct
     1861 attgccgccg gcgccgatgg cgtgatggtg gaggtgcact cccggccaga gcgtgccctc
     1921 aaggatggac cccaggcact gacgctcatc gagttcgccc agttgatgcg ggatctggag
     1981 ccgttcgcgg cggctgctgg ccggccgctg aagcagggcg aagccgaaac ggaataggcg
     2041 cttcgccaaa gagcagatac ggccgcgaga gggcggatgg gatatcgcgc ggtgccctat
     2101 ccgccactct ccccggccgg gggccggcct ggtcccgcgc gcgtctatcc gcctgaaaac
     2161 gcgcccgata gagagcagct gctcctcagg agctcgcgag gccgatctga ccgggcaggc
     2221 cgcgcgtggc gagtgactcc ttcctgcaca ctatggcata ctcatctatt tgcatcattg
     2281 gcatgggtct gcttggcggt tcgctcggca tggctttgaa gcagcgcggg cttgcccggc
     2341 gggtggtggg cgtcgcccga cgcccggagg cgttggagcg cgcccgggag cttggctcca
     2401 tcgacgaggg cgggttggat gccacagcag cggtagccgg cgcggatctc acctttctct
     2461 gtgtgccggt tctcgccatc accaggctcc tgcctctcct tgcgccgcat gtgcgcgccg
     2521 gaggcgtggt taccgacgtg ggcagcacga agaccgccat tgtggacgcg ggcgagagcg
     2581 tgttgcccgg ccgcttcctc gggggacatc cgatggccgg ctcggagcag gcaggcatcg
     2641 agggggcgga cccgcttctc ttcgagggcg ccacatgggc gcttaccccc ggggaggctt
     2701 ccgcgccggt ggagccgctc tccgagttga tcactggatt gggtgcccga ccgcttcttc
     2761 ttccgcccga ggagcatgac cgggccgtcg cggctaccag ccacctgccc catgccacgg
     2821 cggcggcgct ggcccgggtg gttgcggacg cgcttgggga ggctgaggcg gcgccccggc
     2881 ttgctgccgg ctcgtatcgc gatactacgc gcgttgccgc gagcagccca gagctgtggc
     2941 gcgacgtgtg cctgaccaat cgggagccga tcctcgccgc gcttggccgg atgcagggct
     3001 acctggcgga gctggcggag gcccttcggc aagacgacgt ggtggcggtc gaacgcttct
     3061 tccgccaggg aagagaggcg aaggcgcgca tggagagcgc gcgccggaaa ggatagttgg
     3121 tttggcgtca ctccgcattc ccaccgctgg accccttcag ggcgcggcgc gggtgcccgg
     3181 cgataagtcg atctcccacc gcgccgtgat gcttggcgct ctggccgagg gggtcaccga
     3241 gatcgagggg ttcctcccag gggccgattg ccttagcacc atcgcctgct tccgcgcgct
     3301 cggcattcag atcgagcagc cggatccgac tcgcgtcatc gtccatggcg ctggcgaggc
     3361 cggcctgcaa gagccggagg atctgctcta cgtgggcaac tcgggcacca cgctctacct
     3421 caccctgggt gtgctcgccg ggcaggcatt caccagcgtt ttgaccgggg actcctcgat
     3481 tcggcggcgt ccggtgcagc gcgtggggat cccactccgc caaatggggg ccacgatcct
     3541 ggcgcgtcgg gatggcacgc tcccgccggt cacgatccgg ggcggggccc tccaaccgat
     3601 cacctacacg cttccatcgg caagcgccca ggtgaagtcc gccgtcctcc tggccgggct
     3661 gttcgccgag ggcgtcacca ccgccatctc gccggcggtt agccgagacc ataccgagcg
     3721 gatgctacgc ggcttcggcg ccgacgtgcg cgttgagggg atgcgcgcct ccgtgcgcgg
     3781 gccggcgcgg ctgcgcgccc aaccggtctc cgttccggga gacatctctt cggcggcttt
     3841 cctcctggtg gcgggctgca tcgtgccggg ttccgagatc ctggtcgaaa gcgtggggat
     3901 caacccaacg cgttccggca ttgtggacgc gcttcaggcg atgggcgcgc gcatcacggt
     3961 ggagcgcgag agagaggtgg ccggcgagcc ggtggccgac ttgcgcgtct gcgcttccga
     4021 gctgcggggc acgtctctgg ccggtgcgat catccctcgc ctcatcgacg agatcccgat
     4081 cctcgctgtg gctgcggcgt gcgctaccgg tgatacggtg atccgcgacg cgggcgcgct
     4141 ccggatcaag gagaccgacc ggattgcgac gacggcggca ctcttgcgcg cctacggcgt
     4201 ggccgtggaa gtgctggagg atggcatggt ggtgcacggg gctggagacc cgcgtgccct
     4261 gaagggagct gagatcgaca gcgagggtga ccatcgc
//
LOCUS       DAOSCV010000317         4286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12965_length_4286_cov_3.470102, whole genome shotgun sequence.
ACCESSION   DAOSCV010000317 DAOSCV010000000
VERSION     DAOSCV010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12965_length_4286_cov_3.470102"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..>223)
                     /locus_tag="PLY56_08835"
     CDS             complement(27..>223)
                     /locus_tag="PLY56_08835"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ21626.1"
                     /translation="LFEFAMANGAVAGKACGAGGGGCLLFLSAPGRKEDLEMVLRDQG
                     VRVIPFRFDFDGLQVEVKHE"
     assembly_gap    224..233
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<234..986)
                     /locus_tag="PLY56_08840"
     CDS             complement(<234..986)
                     /locus_tag="PLY56_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21627.1"
                     /translation="MIVQSKAPVRIDFAGGWTDVSIFAASAGGAVVNATINKYVHGRM
                     YVLDTEDPVKRRVSGPSGKYVVETDAGEGIEVAYRSDLPAGSGLGTSATLNVVWLSLI
                     KSKVQSDEDRRTIAELSYDLERILGILGGKQDQYASAFGGFNYFTFGEAVGVERLNVA
                     PETVAELESRLVLCYTGKSRLSSGIHGDVWGNYRGGNPDTVNALYSLRNVAIRMRTVL
                     LEGNVEEFGDLLNQNWRHQKALHPSVTNSQIDS"
     gene            complement(1152..2453)
                     /gene="hemL"
                     /locus_tag="PLY56_08845"
     CDS             complement(1152..2453)
                     /gene="hemL"
                     /locus_tag="PLY56_08845"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOJ21628.1"
                     /translation="MARSIQSSRELFRRAQEVIPGGVNSPVRAFRSVGGDPLFIDHGQ
                     GSSLVDVDGNRYIDYVGSWGPLILGHAHPRVVAAAQAAAARGTSYGAPTPGEVEFAEA
                     ICAAVPSVESVRLVNSGTEAVMSAIRLARAYTERERIVKFEGGYHGHFDALLAHGGSG
                     IATLGIPDTPGVPACFAALTHTLPYNDPAAVDSLFAARGDEIAAVIVEPVAGNMGVIP
                     PEPGFLEHLRRVTARHGALLIFDEVITGFRVAPGGAQERYGVTPDLTTLGKVIGGGFP
                     LAAYGGKAEIMARMAPSGPVYQAGTLSGNPVAVAAGLETLRALQRPGFYEELEQKGEE
                     LQAGLEAAAARAGVPATVNRVGSMMTTFFTEGPVRDYAAARRSDTARYAAFFRAMLAR
                     GIYLAPSQFEAAFLSEAHTSGDISATVAAAEEALREVASAG"
     gene            complement(2464..2928)
                     /locus_tag="PLY56_08850"
     CDS             complement(2464..2928)
                     /locus_tag="PLY56_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21629.1"
                     /translation="MDQVIFEEEQWFPDFVTAMAVAAAAGMAVALALLPLARWARPVL
                     ASTASILGTLAVVAPMRTRVTPEGVRVTFGMLGWIPFRIAADQIRSVEPVTYRPLAEF
                     GGWGIRFGRGGKRAYSARGDRGVRIQTPSREYLIGSQRPEALAHALRQIADL"
     gene            complement(3067..3657)
                     /locus_tag="PLY56_08855"
     CDS             complement(3067..3657)
                     /locus_tag="PLY56_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21630.1"
                     /translation="MTPIDEAVGVLRGLASQTNRVEPHCIELRTDPEEGTYLSVEYLC
                     GDLALWVQVQVEEGGQIDENGKVWFTAQLEPISLPQPLGINRLMNLSQRLQTIWPSLR
                     ADVSVRNRGNEECLVEGEWYGDLGPGKDHSLFAAVAAILHEMDAAALSLKEALLDAER
                     MQQVEERVAGLLLGVQHTSPRRRSPAHSKRRRGQRR"
     gene            4022..>4286
                     /locus_tag="PLY56_08860"
     CDS             4022..>4286
                     /locus_tag="PLY56_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOJ21631.1"
                     /translation="MAKQGGEMTVREAGRKGGEIRKRELGSSGYSELGKKGGSRVREL
                     MPWDLAAELAAAEPPLVLDVREPAEYAAAHIAGALHVPRGVLEQ"
BASE COUNT          732 a         1418 c         1422 g          704 t
ORIGIN      
        1 ggatagtgcc agctaaaccg aagcccttac tcgtgcttga cctcgacctg gagaccgtcg
       61 aaatcgaagc ggaaggggat aacgcgcacg ccctggtcgc gaaggaccat ctccagatcc
      121 tcttttcgtc cgggggcaga gaggaagagc agacacccgc cgccgccagc tccgcacgcc
      181 ttcccggcca ccgcgccgtt ggccatcgcg aactcgaaga gggnnnnnnn nnnggagtcg
      241 atctgcgagt tggtcaccga aggatgcagc gccttctgat gccgccagtt ctgattcagc
      301 aggtcgccga actcctcgac attcccctcg agcagcacgg tgcgcattcg gatggcaacg
      361 ttgcgcaggc tgtagagtgc gttgaccgtg tcgggattgc cgccacggta gttgccccac
      421 acatcgccgt ggatgccgct ggagagacgc gacttcccgg tatagcagag gaccaggcgg
      481 ctctccagct cggcgaccgt ctcgggcgcg acattgagcc gctcgacgcc aaccgcttca
      541 ccaaaggtga agtagttgaa gcctccaaaa gcgctcgcgt actgatcctg cttgccgccc
      601 agaatcccca ggatccgctc caggtcgtag gagagctcgg cgatcgtccg gcgatcctcg
      661 tcactttgga ctttcgactt gatcagcgag agccagacca cgttgagggt ggcggatgtt
      721 ccgaggccgg accctgccgg caggtcggag cgataggcga cctcaatccc ctcgccggcg
      781 tccgtctcca ccacatactt tcccgagggg ccgctcacac gccgcttcac gggatcctcg
      841 gtgtcgagca catacatgcg gccgtgaacg tacttgttga tggtcgcatt aacgaccgcg
      901 cctccggcgc tggccgcgaa gatgctgaca tcggtccaac ctccggcgaa gtcgatgcgc
      961 acgggggctt tgctctgtac aatcacgaag gcacctccag agaaacccag gattccgctg
     1021 gcggctctcg atcacacggt cgaggcgctg gttctgggac gtggttttcg aatggtattg
     1081 tatccggttg gcatcatagt gtcaacctgg tagtttcaca gccggcgctt gcacgctggt
     1141 cgcttgaggg ctcatccagc ggaggccacc tcccggaggg cttcctccgc ggctgcgacc
     1201 gtggcggaga tgtcgccgga agtatgcgct tccgagagga acgcggcctc gaactgcgac
     1261 ggcgccaggt agatcccgcg tgccagcatc gcccggaaga acgccgcgta gcgggccgta
     1321 tccgagcgcc gggcggccgc gtagtcgcgc acgggcccct cggtgaagaa ggtcgtcatc
     1381 atcgagccca cgcgattcac ggtggccggc actccggcgc gcgcggcagc tgcttccaga
     1441 cctgcctgga gctcctctcc cttttgttcc agctcctcgt agaatccggg gcgctgcagg
     1501 gcgcgcagcg tctccaggcc cgcggccacg gccaccgggt ttccggagag cgtgcccgcc
     1561 tggtagaccg ggccactggg cgccatgcgt gccatgatct cggcctttcc cccgtaggct
     1621 gccaggggga agccgccacc gatgaccttc cccagagtcg tcaggtccgg ggtcacgcca
     1681 taccgctcct gggcgccgcc gggggccacc cggaatccgg tgatcacttc gtcgaagatg
     1741 aggagcgcgc catggcgggc ggtgaccctc cgcaggtgct ccaggaaccc tggttcaggc
     1801 gggatcaccc ccatattccc ggcgactggt tcgacgatga ccgcggcgat ctcatcgcct
     1861 cgcgcggcaa agagcgaatc cacggcggcg ggatcgttgt agggcagggt gtgggtgagc
     1921 gcggcgaagc acgcgggcac gcccggcgtg tcggggatgc cgagcgtggc gatcccagag
     1981 ccaccgtggg cgagcagcgc gtcaaagtgg ccgtgatagc ctccttcgaa cttcacgatg
     2041 cgctcgcgct cggtgtaggc gcgtgccagc cggatcgccg acatcaccgc ctcggtcccc
     2101 gagttcacca agcggacgct ctcgaccgag ggaacggcgg cacagatcgc ctcggcgaac
     2161 tccacctcgc ccggggtggg ggctccgtag ctcgtgccac gcgcggctgc ggcctgcgcc
     2221 gcggcgacta cacgcgggtg cgcatgtccg agaatcagcg gcccccacga gccgacataa
     2281 tcgatatagc ggttgccatc cacatcaacc agagaggatc cctggccgtg gtcgatgaag
     2341 agggggtcgc ctcccaccga acggaacgct cgcacggggc tattcacccc gccggggatc
     2401 acctcctggg cccggcggaa gagttcgcgc gaactctgga tgcttcgtgc catctttctc
     2461 cccttagaga tcagcgatct gacgcagggc gtgcgcgagg gcctcggggc gctggctgcc
     2521 gatcaggtac tcgcgcgacg gcgtctggat ccgcactccc cgatccccac gcgcggagta
     2581 ggcgcgcttg ccgcctcgcc caaaacggat tccccagccg ccgaactcgg caagtgggcg
     2641 gtacgtcacc ggctccacgg atcggatctg gtcggccgcg atccgaaacg gaatccagcc
     2701 aagcatgccg aaagtcaccc tcacgccctc cggggtgacg cgggttcgca tcggtgcgac
     2761 gacggccagg gtgccaagga tgctggcggt gctggcgagc accgggcgag cccagcgcgc
     2821 cagaggaagc aacgccagcg cgacggccat cccggccgcc gcggcaacgg ccatcgccgt
     2881 gacgaagtcg ggaaaccact gctcctcctc aaagatcacc tggtccattt gccacctcca
     2941 gtggggagca tcgcgatgcc tgacgcgacc atcagatgga gagccggagg gcccaggtgt
     3001 gctggccggg ccgctcacgg ggcgccattg ggtgttgggc cggacaagcc gctttctcgc
     3061 tgccgctcat cggcgctgcc cgcgccggcg cttggagtgt gccggcgagc gcctgcgcgg
     3121 ggaagtgtgt tggactccta gcagaaggcc ggccacccgt tcctccacct gttgcattcg
     3181 ctcggcatcg agcaacgcct ccttcaggct cagagccgcc gcatccatct cgtgcaggat
     3241 ggcggcgacc gcggcgaaga gggagtggtc ctttccaggg ccgaggtcgc cgtaccattc
     3301 gccctcgacc aggcactcct cgttgccacg gttgcgaaca ctgacgtcgg cgcgcagtga
     3361 aggccagata gtctgaagcc gctgcgagag gttcatcagc cgattgatgc caaggggctg
     3421 gggcagcgag atcggctcta gctgcgctgt gaaccacact ttcccgttct cgtcgatctg
     3481 cccgccttcc tcgacctgga cctggaccca cagagccaga tcgccgcaca ggtactctac
     3541 agagaggtag gttccctcct ccgggtcggt tcgcaactcg atgcagtgcg gctctaccct
     3601 gttcgtctgg ctggccaggc cgcggaggac gccgaccgcc tcgtctatcg gtgtcattac
     3661 ttctccctag gcagcgacgg gcggtcccgc cgcggggtac tactctaacg atactattgt
     3721 atcacgcggt acccagtggt caaggggaca tgtgcttgcg gggcgggtac atcaggatga
     3781 aaacggcatg acgcatccgg gaaggggcgg ataggccacc ggggcggcac actgatgtgg
     3841 ctccctcccc ccgttcccgg gccgccgcgt cgaagccgaa acccctctcg cgccagggga
     3901 tgttttccag acctaggctg tgggtaatag cagcgtgatg ggggatgttg gctaaggtga
     3961 tcccccgaag atggcaccgt gcatcagggc acgcttgtga agacacaggg gaggtgaaaa
     4021 catggcaaag caaggtggcg agatgaccgt tcgtgaggcg gggcgcaagg gcggtgagat
     4081 ccggaagcgc gagctgggga gcagcggcta ctccgagctc ggcaagaagg gtggcagccg
     4141 cgtgcgcgag ctgatgccct gggacctggc cgccgagctc gcggcggccg agccgccgct
     4201 cgtgctcgac gtgcgcgagc cggccgaata cgccgccgcg cacatcgccg gtgcgctgca
     4261 cgtgccgcgc ggcgtgctcg agcagg
//
LOCUS       DAOSCV010000318         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12977_length_4284_cov_3.283991, whole genome shotgun sequence.
ACCESSION   DAOSCV010000318 DAOSCV010000000
VERSION     DAOSCV010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12977_length_4284_cov_3.283991"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLY56_08865"
     CDS             <1..217
                     /locus_tag="PLY56_08865"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HOJ21632.1"
                     /translation="RRAVKAMEARIAAAEARMQELYNILASGSTYTDGDRVREVHAEY
                     EALNNSLPELYAEWERLAEAAEAISAR"
     gene            353..679
                     /locus_tag="PLY56_08870"
     CDS             353..679
                     /locus_tag="PLY56_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ21633.1"
                     /translation="MADYNKVGLLAIREDRILLCQKRGLSRLILPGGTLEPGETPEAC
                     LHREIREELGDVVARDLEYLGTYRDRAASDDPSVVKTVEIQLYRGEIAGQPVASRDRG
                     AHLVRS"
     gene            764..2602
                     /locus_tag="PLY56_08875"
     CDS             764..2602
                     /locus_tag="PLY56_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010673279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOJ21634.1"
                     /translation="MTDSVELLAPARDLECGRTAIDCGADAVYIGAPRFGAREAAGNS
                     LDDIAALVEHAHLYWARVYVTLNTLLRDDELPEAVRLAEQLYALGVDGLIIQDVGLLE
                     CDLPPLPLISSTQMHNATPERVAFLEKVGFQRVILARELTLDQIREIRRQTRIELEYF
                     VHGALCVSYSGQCYLSYALGGRSGNRGQCAQPCRKPYSLVDARGKTLVRRAHLLSLRD
                     LNLSEHLRPLLEAGVRSLKIEGRLKDRAYVANVVAHYRALLDELLPAMGLRRSSSGMA
                     RPGFQPDVSKTFNRGGTTYFLYGRGEQIGAHDTPKMKGERLGKVTRVTPRGIYLEGNA
                     TASSGDGICFFDRRGELRGTRVHGSREGALLLASMEGIAPGTVVYRNHDHEFLTQVAK
                     SRPQRRVAVWMTLERNAEGLSLTTVDEDGIRAQGTLACPLEPAQKPEQALATIRAQLA
                     RCGDSEFECAEVAVALDDVPFVPLSALNALRRDTLQQLARARAAARPILRGGIKRNEV
                     AFPERTLSFEGNVLNRWAEAFYRRHGVTTIEPAAESGLDLRGRRVMTTRYCLKHELGL
                     CARDGARVRQAEPLSLVDDDGRRLILRFDCARCEMEVYLPEAAPPS"
     gene            complement(2734..3192)
                     /locus_tag="PLY56_08880"
     CDS             complement(2734..3192)
                     /locus_tag="PLY56_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ21635.1"
                     /translation="MEQSDFVSTDTEESFSFLLSQAARAVARDLQARLHPYGVTPAQF
                     NVLSCLWEDDALPANVLAERSGFDAPTITGILDRLERQELAVRRRGSDDRRVVLACLT
                     ERGSALRSALPEIVRAADQAALDGLDPERVALLRHALKQVIANLSSAPRR"
     gene            complement(3245..>4284)
                     /locus_tag="PLY56_08885"
     CDS             complement(3245..>4284)
                     /locus_tag="PLY56_08885"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ21636.1"
                     /translation="ETFRGYGPERGYAFLAEAIAAHDFAPRGIDLAVDEIFISDGAKS
                     DTANIQELFAAESIVALTDPVYPVYVDSNVMAGRAGTLGDEGRYDRLVYLPCTAENNF
                     RPPLPESHVDLIYLCYPNNPTGAVATRAMLEEWVAYAQREKAVILYDAAYEAYIRDDA
                     LPHSIYEIPGAREVAIEFRSFSKTAGFTGTRCAYTIVPKELYAYAPDGTPVSLNALWD
                     RRQSTKFNGVPYVVQAGAAAVFTPEGQRQVREVIDFYMENARIIREGLSSAGLTVYGG
                     VHAPYIWVKTPDGLSSWEFFDRLLNEVNVVGTPGVGFGPSGEGYLRLTAFGKREDAEE
                     AVARIRARLSA"
BASE COUNT          726 a         1353 c         1491 g          714 t
ORIGIN      
        1 gcgtcgggcc gtgaaggcga tggaggcgcg catcgccgcc gcggaggcgc ggatgcagga
       61 gctctacaac attctggcct ctggctccac ctataccgat ggcgaccgcg tccgggaagt
      121 gcatgccgag tatgaggcgc tcaacaactc gctgccggag ctctatgccg agtgggagcg
      181 cctggccgag gccgccgagg cgatcagcgc ccgatagtag agaggccccg gactcgcggg
      241 taatagccgc caagccgtcg tgagcgaggc cgttcctcac gcgggtctgt gcaaggctcc
      301 tcaagagtgt gaggcgcgtg tattctggaa ccccacctgg agagggggat taatggcgga
      361 ttacaacaag gtcgggctgc tggcgattcg cgaggaccgg atcctgctct gtcagaagcg
      421 cgggctctcg cggctcatcc tgcccggcgg gaccctggag ccgggcgaga cgcccgaggc
      481 gtgcctgcac cgcgagatcc gtgaggagct gggcgatgtc gtcgcgcgcg atttggagta
      541 tctcggcacc taccgtgatc gcgccgcatc cgatgacccc tcggtggtga agacggtcga
      601 gatccagctc taccggggcg agatcgcggg ccagcccgtg gcctcccgag atcgaggcgc
      661 tcatctggtt cggtcgtgag agtgaccgcg agctgctctc gccgatcctc atcaacgcca
      721 tcctgccgga cctggcccgg cggggagtgc tgaggtgggg tgaatgaccg attccgtcga
      781 gctcctggcg ccagcccgcg acctggagtg cgggcggacg gcgatcgatt gcggcgccga
      841 tgccgtgtac atcggggccc cacgcttcgg cgcccgggaa gcggcaggta acagcctcga
      901 cgatatcgcg gcgctcgtgg aacacgcgca tctctactgg gcgcgggtct acgtgacgct
      961 gaacacgctc ctccgcgacg atgagcttcc cgaggcggtg cgcctggcgg aacagctcta
     1021 tgccctcggg gttgatggcc tgatcatcca ggacgtaggc cttctggagt gcgacctgcc
     1081 gcccctaccg ctgatttcca gcacgcagat gcacaacgcc acgcccgagc gagtggcgtt
     1141 cctggaaaag gtaggcttcc agcgggtgat cctggcgcgc gagctgacgc tcgaccagat
     1201 ccgcgagatc cgccggcaaa cgcgcattga gttggagtac ttcgttcacg gtgcgctctg
     1261 cgtatcatac agcggccagt gctatctcag ctatgccttg ggcgggcgca gcggcaaccg
     1321 ggggcagtgc gcgcagccgt gccgcaaacc gtactccctg gtagatgccc gtgggaagac
     1381 gctggtacgc agggcgcatc tcctctcact ccgcgatctg aacctgagcg aacacctgcg
     1441 cccgctcctg gaagccggcg ttcgctcgct aaagatcgag ggccggctga aagatcgggc
     1501 ctacgtcgcg aacgtggtcg cccattaccg cgccctgttg gatgagctgt tacccgcgat
     1561 gggcctgcgg cgcagctcgt ctgggatggc gcgcccgggt ttccagccgg acgtgagcaa
     1621 gacgttcaac cggggcggca ccacctattt cctctacggg cgtggcgagc agatcggcgc
     1681 gcacgatacg ccgaagatga aaggagagcg cctcgggaag gtgacgcgcg ttaccccgcg
     1741 aggcatctac ctggagggga acgcgaccgc aagtagtggg gatggcatct gctttttcga
     1801 ccgcaggggc gagctacgcg gcacgcgggt ccacggtagc cgcgagggcg cgctgctcct
     1861 cgcctcgatg gaggggattg cgcccggcac ggtcgtctac cgcaaccacg atcacgagtt
     1921 tctgacgcag gtggcaaaga gccgcccgca gcgccgggtg gccgtctgga tgacgctgga
     1981 gcggaacgcg gagggcctct cactgacgac ggtggatgag gatggcatcc gagcccaagg
     2041 cacgcttgcc tgtcctctgg agccggcgca gaagccggag caggcgctcg ccacgatccg
     2101 cgcgcagctg gcgcgctgtg gcgactccga gtttgagtgc gcggaggtgg cggtggcgct
     2161 ggatgatgtg ccgttcgtgc cgctctcggc gctcaacgcc ttgcgtcgtg acacgctgca
     2221 gcaactggcg cgggcgcgcg cggcggcacg ccccattctg cgcgggggca tcaagcgcaa
     2281 cgaggtcgcc tttcccgagc ggacgctctc cttcgagggg aatgtcctca accggtgggc
     2341 ggaagcgttc taccgccggc acggcgtgac gacgatcgag cccgcggcgg aatcggggct
     2401 cgacctgcgc ggccggcggg tgatgacgac gcgctactgc ctcaagcatg agttagggct
     2461 gtgtgccagg gatggcgcga gagtccgtca ggccgagccg ctctccctgg tggatgacga
     2521 tggtcggcgc ctgatactgc gcttcgattg cgcgcgctgc gagatggaag tgtacctacc
     2581 cgaagcggcg ccgccctctt gaggggcggc accgctcaac acacctgcgg atagggcact
     2641 ggccgaaacg tgggaggcgt ggagacctct atcgcatccg ggcgaagagg gccggaagtg
     2701 cgcgaatgga gcccctgcca cttcacgtca tgctcacctt ctcggagcgc tcgaaaggtt
     2761 tgcgatcacc tgctttaggg cgtggcgtag gagggcaacc cgctcggggt ccaggccgtc
     2821 gagggcggcc tggtcggcgg cgcgcacgat ctccgggaga gcgcttcgaa gagcggagcc
     2881 acgctctgtc aggcaggcaa gaaccactcg tcggtcgtcg ctcccgcggc gtctcactgc
     2941 cagctcctgg cgttccaggc ggtccaggat gccggtgatc gtcggggcgt cgaacccgga
     3001 acgctcggcg aggacattcg ccggcagggc atcatcctcc cacaggcagg agaggacgtt
     3061 gaactgcgcc ggagtaacgc catagggatg aagccgggcc tgcaggtcgc gcgcgacagc
     3121 gcgcgcggcc tgggagagga gaaaactgaa gctctcctcg gtatcagtcg aaacgaagtc
     3181 gctctgctcc atagttcaca ccgcgatact ctcggacgcc ctctcctggc agcccatggt
     3241 ggcctcaagc agagaggcgc gcgcggatgc gcgcgacagc ctcctcggca tcctctcgct
     3301 ttccgaacgc tgttaggcga agatagccct cgccgctcgg cccgaagccc acgcccggcg
     3361 tgcctaccac attgacttca ttcagcagcc ggtcgaagaa ctcccaggaa gagaggccgt
     3421 cgggcgtctt gacccagata tagggggcgt ggacgccgcc gtacacggtg agacccgcgc
     3481 ttgacaggcc ctcgcggatg atccgtgcat tttccatgta gaagtcgatc acctcgcgca
     3541 cctgccgctg gccttccggg gtgaaaacag cggcggcgcc cgcctggacc acgtagggca
     3601 ccccgttgaa cttggtgctc tggcgtcggt cccatagtgc gttgaggctc acgggagtgc
     3661 catctggcgc gtaggcgtag agctccttgg gcacgatggt ataggcacag cgggtgcccg
     3721 tgaagccggc ggtcttggag aagctgcgaa actcgatcgc cacttcgcgg gcgccgggaa
     3781 tctcgtagat gctgtgcggg agggcatcgt cgcggatgta cgcctcgtaa gcggcatcgt
     3841 agaggatcac cgccttctcg cgctgcgcgt aggccaccca ctcctccagc atggcgcggg
     3901 ttgccacggc gcctgttggg ttgttgggat agcacaggta aatcagatcg acatggctct
     3961 ctggcagcgg cggccggaag ttgttctccg ccgtgcaggg gaggtagacg agccggtcgt
     4021 agcgcccctc gtccccgagg gtgcccgcgc gccctgccat cacgttgcta tcaacgtaga
     4081 cgggatagac cgggtccgtc agcgccacga tgctctcggc ggcgaagagc tcctggatat
     4141 tcgccgtatc gctcttggcg ccatcgctga tgaaaatctc atcgactgcg aggtcaatgc
     4201 cacgaggtgc gaagtcgtgg gcggcgattg cctcggcgag gaacgcatag ccgcgctcgg
     4261 ggccatatcc acgaaaagtc tcgg
//
LOCUS       DAOSCV010000319         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_12992_length_4280_cov_3.293018, whole genome shotgun sequence.
ACCESSION   DAOSCV010000319 DAOSCV010000000
VERSION     DAOSCV010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12992_length_4280_cov_3.293018"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLY56_08890"
     CDS             complement(<1..246)
                     /locus_tag="PLY56_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21637.1"
                     /translation="MLRRGNETMEFPGNTLYGTAPEPYEIEEDRAGGALVLRTPFYAI
                     EHDLRAGGAIRAIRLFQGSGENLLLAPMGCEVVLEGDA"
     gene            complement(293..3631)
                     /locus_tag="PLY56_08895"
     CDS             complement(293..3631)
                     /locus_tag="PLY56_08895"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOJ21638.1"
                     /translation="MPYPRLAPGFRALFLFLIATGVACLPAWVGAAPLPPPCRHVPVD
                     QEISLEEVLRLVDPGHPAMREVVAARDAGNLTRARDLLARHMAARERPVVPPTRFPGL
                     GPGNSTLILPGSAADRARVDQTTMRHRFTLRNNDAGTVETYDLGPRIEWMKNPGKALS
                     WILYLNQLNHLAELAGVYRDTRDEKYAAEIVAQLSSWTQQCCRCYGYMRAERMVPSGM
                     EVRNRLCNMVAAYDVVRRSPSLTPDAHLTFWKFFIACARELMRYGGTSYPGLLPLAVI
                     FPEFQESRAWFEAGAANLRAMIVDRTSPEGAWDTHSISYQTVSVPWAARVLEFLDANP
                     GAPGVDELAAMVKRQGGKLLSAPLWVVMPNGGLPNIGDTYGRPDWDGSTAQGFLRSYL
                     AGFTGLPPAEEARLSNIADPYERLLATLAAVSGSDGPVPAVTSVALPGSGWYAMRGGW
                     NRDAAYLYFDLSTQAIGHAHEDACHFDLYAYGKPLLTDTGDYFLGWGYRTALHNTVEV
                     DGRSQARGANAPMIPIEWVTTRDFDFVDGAHGGYGDLGVTYRRKILFVKPGYFVLCDL
                     LTGAGRHLLEQFFHFAGPAQTLPAEVSLDPKTGRARTLHANTANVEVIPAYTAGLRSG
                     FAEAVETDMRVEDQRERKAMLGWMVTTGSFQRVKSPVAVYTREGDLPQAFYDVLFPTP
                     RGGEASVTLRELPVEEDGRSLSPHEAAGLEIRCRMARPAPEAESAQMEMGPNLALGKP
                     GFAEISRGQFPEGAKLLTDGDTGPRTISAALSSAPHQPGVPLTGRFGVDFGTPVAVNA
                     VVLHHGIWNGKTILYPAEKMSIQYWDGRAWHDVTGARTSWHDDQVSRTFFDTVRTTRL
                     SVAVERAGGGRLALREFEAYHVSSEELARFERARQARVTEEWTDVILISHRGARARRY
                     GDYLFDGEIALIRKDAAGRVARVGVKTGKSVREGGRTLLESPELLDSFCAEWQGEAVR
                     LDGPAPRGLALLAQNARRKECAGSALAAEPRGGALVVTRAPQAAPPRITEVRVRLEPP
                     QKGLAGAQPFAWVTWKTDVPATSRVEYIAEGQPARRTPVDAALKREHRVRVDFLVPGE
                     EYSFRAISVDAWGQRAEAPATRAD"
     gene            4097..>4280
                     /locus_tag="PLY56_08900"
     CDS             4097..>4280
                     /locus_tag="PLY56_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21639.1"
                     /translation="MRASLNLPHLALALTALAAATPGAEAAEPPLDPDRVAAIAAMLP
                     ERPEGLGRPISDRAAWE"
BASE COUNT          669 a         1527 c         1419 g          665 t
ORIGIN      
        1 cgcgtcgccc tccaagacaa cctcgcaccc catcggcgcc aggagcaggt tctcccccga
       61 gccctggaac agccggatcg cgcggatcgc cccgccggcg cgcaggtcgt gctcgatggc
      121 atagaaaggc gtgcgcaaaa cgagcgcccc gcccgcgcga tcttcctcaa tctcatacgg
      181 ctccggcgcc gtgccgtaaa gcgtgttccc tggaaactcc atcgtctcgt tgccccttct
      241 caacaccgcc ggccctctgg catctctccc gagcgcccac gctaggccgg gtctaatccg
      301 cgcgcgtggc gggcgcctcg gccctctggc cccaggcatc gaccgagatc gcgcggaagg
      361 agtattcctc gcccggaacc aggaaatcga cccggacgcg gtgctcccgc ttcaacgccg
      421 cgtcgaccgg cgtgcgccgc gccggctggc cctccgcgat gtactccacg cgcgaagtgg
      481 ccggcacgtc cgtcttccag gtgacccacg cgaacggctg cgcgccggcc agccccttct
      541 ggggcggctc caggcgaacg cgcacctcgg tgatccgggg cggcgcggct tgcggagcgc
      601 gggtcaccac cagtgcgccg ccgcgcggct ctgccgcgag cgcgcttcca gcacactcct
      661 tgcgccgggc attctgcgcg aggagcgcca ggccccgcgg cgccggacca tcgaggcgga
      721 cggcttcgcc ctgccactcc gcgcagaagc tgtccaggag ctccgggctc tccaggagcg
      781 tccgccctcc ctcgcgcacg ctcttccccg tcttcacgcc cacgcgggcc actctccccg
      841 ccgcgtcctt acgaatgagc gcgatctcgc cgtcgaagag gtaatcgcca taccggcgcg
      901 cgcgggcgcc gcggtgggag atcaggatga cgtcggtcca ctcctcggtc accctcgcct
      961 gacgggctct ctcgaagcgc gcaagctctt ccgacgagac gtggtaggcc tcaaactcgc
     1021 ggagcgccag ccggcctccc cccgcgcgct ccacggccac gctcagccgg gtggtgcgca
     1081 cggtgtcgaa aaacgtccgg ctcacctggt cgtcgtgcca gctggtccga gcgccggtga
     1141 cgtcgtgcca ggctctccca tcccaatact ggatgctcat cttctccgcg gggtagagga
     1201 tcgtcttccc attccagatg ccgtggtgca aaacgaccgc gttcaccgcg actggggtgc
     1261 caaaatcgac cccgaaacgc cctgtgaggg gcacgccggg ctggtgcggc gccgaggaga
     1321 gtgccgcgct gatagtgcgt gggccggtat cgccatcggt caggagcttc gcgccttcgg
     1381 ggaactgccc ccgcgatatc tcggcgaacc ctggctttcc gagggcaagg ttggggccca
     1441 tctccatctg agcgctctct gcctctggcg ccggacgcgc catccggcag cggatctcca
     1501 ggccggcggc ctcatgcggc gagagcgacc tcccatcctc ctccacgggc agctcgcgga
     1561 gcgtaaccga tgcctctcca ccgcgcggcg tggggaagag cacgtcatag aacgcctgcg
     1621 gcaggtcgcc ttcccgggtg tagaccgcca cgggcgattt cacccgctgg aagctccccg
     1681 tggtgaccat ccacccgagc attgccttgc gctcgcgctg atcctccacc ctcatatcgg
     1741 tctccaccgc ctcggcgaag ccggagcgca gcccggcggt gtaggcgggg atcacctcca
     1801 cgttcgccgt gttcgcgtgc agagtgcgcg cgcgtccggt tttcggatcg aggctcacct
     1861 ccgcaggcag tgtctgagcc ggcccggcaa agtggaagaa ctgctcgagg aggtggcgcc
     1921 cggcgccggt gaggaggtcg cagaggacaa agtagcccgg cttcacgaag aggatcttcc
     1981 gccggtaggt cacgcccagg tcgccatagc cgccgtgggc gccatcgacg aagtcgaagt
     2041 ccctcgtggt gacccattcg atgggaatca tcggggcgtt cgcaccccgc gcctgtgagc
     2101 gcccgtccac ctccaccgtg ttgtggagcg ccgtgcggta gccccacccc aggaagtagt
     2161 cgccggtatc ggtcaggagc ggcttcccgt aggcatacag gtcgaagtgg caggcgtctt
     2221 catgggcgtg cccgatggcc tgggtcgaga ggtcgaagta aagataggcg gcatcgcggt
     2281 tccagccgcc gcgcatcgcg taccacccgg atccgggcag ggcaaccgag gtgaccgcgg
     2341 gcaccgggcc gtcgctcccc gagacagcgg ccagcgtggc cagcaggcgt tcataggggt
     2401 cggcgatgtt gctgaggcgc gcttcctccg ccggcggcaa tccggtgaac ccggccaggt
     2461 aggagcggag gaagccctgg gccgtgctcc catcccagtc cggccggccg taggtatcgc
     2521 cgatgttggg gaggccgccg ttgggcatca caacccagag cggggcgcta aggagcttgc
     2581 ccccttgccg cttcaccatc gccgcaagct cgtccacgcc gggcgcgccc gggttggcat
     2641 ccaggaactc gagcacccgc gccgcccacg gcaccgagac cgtctggtag gagatggagt
     2701 gggtatccca ggcaccctcg ggcgaggtgc ggtccacgat catggcgcgc aggttagccg
     2761 cgcccgcctc aaaccaggcg cgcgactctt ggaactcggg gaagatcacc gcgagcggga
     2821 gcagcccggg ataggaggtg cccccgtagc gcatcagctc gcgcgcacac gcgatgaaga
     2881 acttccagaa ggtgaggtgc gcatccggcg tgagcgaagg cgagcggcgg accacgtcgt
     2941 aggcggccac catgttgcag agccggttgc gcacctccat gcccgaggga accatccgct
     3001 cggcgcgcat gtagccgtag caccggcagc actgctgcgt ccagctggag agctgcgcga
     3061 cgatctcggc ggcgtatttc tcgtcgcgcg tgtcgcgata gacgccggcc agctccgcca
     3121 ggtggttgag ttggttcagg tagaggatcc agctgagcgc tttgcccggg ttcttcatcc
     3181 attcgatgcg cggcccgagg tcgtaagtct ccaccgtgcc ggcgtcgttg ttgcgcaggg
     3241 tgaaccggtg gcgcatcgtc gtctgatcga cgcgcgcgcg gtcggcggcg ctcccaggga
     3301 ggataagcgt ggagttaccc ggaccaagcc ctggaaaacg cgtgggcggc accacgggcc
     3361 tctcgcgggc ggccatgtgc cgggctaaaa ggtcgcgagc gcgcgtgaga ttgcccgcgt
     3421 ctcgcgccgc caccacctcg cgcatcgcgg gatggccggg atcgaccaga cggagcacct
     3481 cctccagcga gatctcctga tcgacgggca catgcctgca cgggggcggc aggggcgccg
     3541 ctccaaccca cgcgggcaag cacgccacgc cggtggcaat cagaaacaga aacaaagccc
     3601 ggaagccggg cgccaatcgc gggtatggca tccagtcact ctcctcgcgg cggtcgcggg
     3661 ccgccgggga atcgttcggc cccaagagga tatccccttc cgggtgttca ttatccgcag
     3721 ctgggctatg gcgggcattc gcctaactcc caaccccgtg ccatccccaa ggaagggggg
     3781 ctaccgcatg gggctcgcgg gcacctccga agggaacacg ccccccaggg tgcccgtcct
     3841 taccattcgc gcccgtcctt ctatccgcgg tatcggtgcg gtcaattggc agctcgattt
     3901 gcttgactct cccccacggg cctgttatac tccctgcaaa gcggtggctg gcgatggccc
     3961 ggctgccgcg tggatagtgg cacagaacga aaccggagag gaggccgggc gttgcagggg
     4021 atgatttctc tctccctcgc gcaccccgca ttcggagggg cgagcgatcc ctcccctgcc
     4081 cccgggtcgg tctgaaatgc gcgcttccct caatttacct cacctggctc tcgcactcac
     4141 agcactcgcg gcagcgacac ctggggcaga agcagccgag ccgccgctcg atcccgaccg
     4201 cgtcgcggcg attgcggcga tgctcccgga gcgccccgag ggtctgggcc gtccgatctc
     4261 cgatcgcgct gcctgggaac
//
LOCUS       DAOSCV010000320         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13145_length_4242_cov_2.652496, whole genome shotgun sequence.
ACCESSION   DAOSCV010000320 DAOSCV010000000
VERSION     DAOSCV010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_4242_cov_2.652496"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..>4242
                     /locus_tag="PLY56_08905"
     CDS             100..>4242
                     /locus_tag="PLY56_08905"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOJ21640.1"
                     /translation="MFGSKRKQPLGTRERPYLNLTNPGQRPGGGRAIPVQNVNLTRSP
                     ADDIDAVWSPDGTKILFASNGEDTNGDMRIDKVRDDGTYHIWIMNPDGSGQQQLTHDP
                     GNQVEPAWSPNGLRIAYASNEEGDYEIYLLTLSTREKVKLPVGPSEERHPTWAAGGDT
                     LAFSSDREGLPKIYSMKADGRHLTPLTKGPSEDRNPVWSPDGQSILFESNGVDSDGDG
                     VIDATTNTTRVWRMSYFGSQQQQLTAFEVPDGTVIDRNPAWNSDGRVIFFSSNRKDAN
                     GDGTWETVGDFDVYEYPLVIQGGVVANAETPNSSSAIKRTQLSPSEIEPLTGNEDHPG
                     LFPIVDFKNPKVMYVTDQAGARDIWLTSITDVTDPRLEALPEVSPKLTIPGSSVVITA
                     RVSDLESGVKAVYAQIRDPDDAVTDAQGIDHKVYYVQPDNPDSATNPMPIWTEVDAHM
                     VHATNYTYHIPPYQPRMDDLSALDFAMPPHDGWLQLYDDGDPKHGDAVAGDGIYSNSW
                     KTPTNAQSDFYVDIIAYDNAGNWKIYDGITCFSTQQFKAMHGILLVLDYVHGQKFMQG
                     DRSNFTYASEYFPMWFPVESYYTRNPTGMPASLINLGAYANNIINIDDFGGVPPSPPA
                     LSAAPTDTFIRNTAEEGYDIWRVVCRGRLPPDVLAAYLPRVVEQPDFQDPYNAVRQQL
                     VADRGIVWASPYSGNLWVGEGTIVDGDTQAKLINFLDRGGRLVVTGQDVAWALTRDGI
                     VKDPPLLARMGVQYIDDAPSWTLTGGAADDRYFLTGAGGQVTTQPWATHWHAEAQPTA
                     LQMPVCFFPVIQAWGSTPSLSISVGSTSAREQGNNTSTDAAGNQFWMDGILPVAPSQT
                     LYSYRSPNTGISGRTDAVAAVYRIDPEVNSRSAFFAFGFEGIHRKYASHGSYGTHCEN
                     LRHNLMHNLICWMRTGTFYGLVQFTAGLGPAKGALVQVFEESIPLNEERLVAQAFTRE
                     DGTYEVVGLPPRVYRVRALLPGYTYDHAQRAAVHGAGREEINFRLSEAPAGSISGKVS
                     EQDGVTPIEGATITVTLQGEYKGEEIVRTVTTDQMGIYAVTDLPTGQYRVTCVADGFA
                     EQQYPTLVTVNPGTEAQNINFVMGGMPGGVVGTVTRQSDGQVVAGATVELRSGTTLVA
                     NTVTDDTGQFAFQNVPAGTYELTVTGAAGILPTSVTVVVEANAQVRRNVVANTPPPAQ
                     VAGMVTDALGNPVTGAKVDVVSGGTVIKTAVSGDVQTEGTNQFNYKITGLAAGNYEIR
                     ASKGGFTGSSASVRLSESQQLFNVNFTLESLHVYSSGLTMVSAPYDYSDVDAANFLGL
                     PANQTKVASWVGNRYAFYPENPADRFRIGKGYFLLLEKPVVLTREGKAADTSKPATLN
                     LTA"
BASE COUNT          813 a         1347 c         1384 g          698 t
ORIGIN      
        1 gggggcggat cggttcgcct cccagggggt tctgcgcctg tggccacgcg cgccgggcgc
       61 gcgcctgtca cctccgcaat ggcgacaggc cttccaaggg tgttcgggag caagcgaaag
      121 caacccctgg gcacgcgcga gcggccctat ctgaacctga ccaacccggg gcagcgcccg
      181 ggcggtggcc gcgcgatccc ggttcaaaac gtgaacctca cccgctcgcc ggcggatgat
      241 atcgatgcgg tctggtcgcc ggatggcacg aagatcctct tcgccagcaa cggcgaggat
      301 acgaacggcg atatgcgcat cgacaaggtg cgcgacgatg gcacctacca catctggatc
      361 atgaacccgg atgggtcggg ccagcagcag ttgacccacg atcccggcaa ccaggtggag
      421 cccgcctggt ctcccaacgg cctgcgcatc gcctacgcct ccaacgagga aggcgattac
      481 gagatctacc tgctcacgct ctcgacccga gagaaggtca agcttcctgt cggaccgagc
      541 gaggagcgac atcccacatg ggctgccggt ggcgatacgc ttgccttctc gagcgaccgc
      601 gagggcctgc cgaagattta tagcatgaag gcggatggcc gccacttgac gccgctcacc
      661 aagggcccga gcgaggatcg caacccggtg tggtctcccg atggccagag catcctcttc
      721 gagtcgaacg gtgtcgatag cgacggcgac ggcgtgattg atgcgaccac gaacaccacc
      781 cgcgtgtggc gcatgtccta tttcgggtcg cagcagcagc agttgaccgc gttcgaggtg
      841 cccgacggca cggtgatcga ccgcaatccg gcgtggaaca gcgacggccg cgtcattttc
      901 ttctcgtcca accgcaagga tgccaatggc gacggcacct gggagaccgt gggcgatttc
      961 gacgtctacg agtatcccct cgtcattcag ggcggcgtgg tggccaacgc ggagacgccg
     1021 aatagcagct cggcgatcaa gcgcactcag ctctcgccca gcgagattga gccgctcacg
     1081 gggaatgagg accatcccgg gctcttcccg atcgtggatt tcaagaaccc gaaggtgatg
     1141 tacgtcaccg accaggcggg agcacgcgac atctggctca cctcgatcac cgacgtcacc
     1201 gatccgaggt tggaagcgct gcctgaagtc tctcccaagc tcacgattcc cggatccagc
     1261 gtggtgatca cggccagggt gagcgatctg gagagcggcg tcaaggccgt gtatgcccag
     1321 atccgcgatc cagatgacgc cgtcacggat gcccagggga ttgaccataa ggtctattac
     1381 gtccagccgg acaatcccga ttccgcgacc aaccccatgc cgatctggac ggaagtcgac
     1441 gcgcacatgg tccacgcgac caactacacc taccacattc cgccctatca gccgcgcatg
     1501 gatgacctgt cggcgctcga tttcgctatg ccgccgcacg atggctggct tcagctttac
     1561 gatgacggtg acccgaagca tggcgatgcc gtcgcagggg atgggatcta cagcaattcg
     1621 tggaagacgc ctaccaacgc ccagagcgac ttctacgtcg atatcatcgc ctacgacaac
     1681 gccgggaact ggaagatcta cgacggcatc acctgcttca gcacgcagca gtttaaggcg
     1741 atgcatggaa tcctgctcgt gttggactat gtccacgggc agaagttcat gcagggcgac
     1801 cggtcgaact tcacctacgc cagcgagtac ttcccgatgt ggttcccggt ggagagctac
     1861 tacacccgga acccaaccgg aatgccggcg tcactcatca atttgggcgc ctacgcgaat
     1921 aacatcatca atatcgatga cttcggcggg gttccgccca gcccgccggc gctctcggcg
     1981 gcgccaaccg acaccttcat ccgaaacacg gcggaggagg gctacgatat ctggcgggtg
     2041 gtgtgccgcg gtcggctgcc accggatgtg ctcgccgcgt atctgccgcg cgtcgtcgag
     2101 cagcccgact tccaagatcc gtataacgct gtccggcagc aactcgtcgc ggacaggggg
     2161 atcgtgtggg cgagtcccta ctcggggaac ctatgggtcg gcgagggtac catcgtcgat
     2221 ggcgacaccc aggcgaagct gatcaacttc ctggatcgtg gcggccggct cgtggtcacc
     2281 ggccaggacg tggcctgggc gctgacccgc gatggcatcg tgaaggatcc gccgctgctc
     2341 gcccgcatgg gcgtgcaata tatcgatgat gccccttcgt ggacactcac gggcggagcg
     2401 gccgatgacc gctacttcct gaccggtgcg ggcggccagg taaccaccca gccgtgggct
     2461 acccactggc acgcggaggc gcaaccgacc gcgctgcaga tgccggtctg cttcttcccg
     2521 gtcatccagg cgtggggctc gacgccgtcg ctctcgatct ccgttggctc gacgtccgcg
     2581 cgcgagcagg gcaacaacac gagcacggac gcggcgggca accagttctg gatggatggc
     2641 atcctgccgg tggcgccttc gcagacgctc tactcctacc ggtcgcccaa cacggggatc
     2701 agcgggcgca ccgatgcagt tgccgcggtc taccggatcg atccggaggt caactcgcgg
     2761 agcgcgttct ttgccttcgg gttcgagggg atccaccgca agtacgcgtc gcacggctcg
     2821 tatgggacgc actgcgagaa cctgcggcac aacctgatgc acaacctgat ctgctggatg
     2881 cgcacgggca ccttctatgg gctggtgcaa ttcacagcgg ggttggggcc ggctaagggc
     2941 gccctggttc aggtgttcga agagagcatc ccgctcaacg aggagcggct cgttgcgcag
     3001 gcgttcacac gcgaagatgg cacctatgag gtggtaggcc tgccgccgag agtctatcgc
     3061 gtgcgcgcgc tcctgcccgg ctatacatat gaccacgcgc agcgcgccgc cgtgcatggc
     3121 gcgggccgag aggagatcaa cttccggctg agcgaggcgc ccgccgggtc gatctccggc
     3181 aaggtgagcg agcaggatgg cgtcaccccg attgagggcg ctacgatcac ggtcaccctg
     3241 cagggcgagt acaaaggcga ggagatcgtc cgcaccgtca cgactgacca gatgggcatc
     3301 tacgcggtga cggacctgcc gaccggccag taccgggtga cctgcgtcgc cgatggattc
     3361 gccgagcagc agtatcccac cctggtcacc gtgaatccgg ggaccgaggc tcagaacatc
     3421 aacttcgtca tgggcgggat gcctggcgga gtggtgggta cggtgacacg gcagtccgat
     3481 ggccaggtgg tggccggtgc cacggtggag ctgcggagcg gcaccacgct cgtcgcgaac
     3541 acggtgacgg atgacacggg ccagttcgcc ttccagaatg tgccggcagg cacctacgag
     3601 ctcacggtta cgggagctgc agggatcctg ccaacgagcg tcaccgtggt agtggaagcg
     3661 aacgcccagg tgcgccgcaa cgtggtggcg aatacgccgc cgccggcgca ggtagccggt
     3721 atggtcaccg atgccctggg caacccggtc acgggcgcga aggtggacgt ggtcagcggc
     3781 ggcaccgtga tcaagacggc ggtctccgga gacgtgcaga cggagggcac gaatcagttc
     3841 aactataaga tcacggggct tgctgcaggc aactatgaga tcagggcgag caagggcggg
     3901 ttcaccggga gcagcgcctc ggtgcggctc tcggagagcc aacagctgtt caacgtgaac
     3961 ttcaccctgg aatcgctcca cgtctattcg agcggcctga cgatggtctc cgcgccgtac
     4021 gactacagcg atgttgacgc cgccaacttc ctcggcctgc cggcgaacca gacgaaagta
     4081 gcgagctggg tcggcaaccg gtacgcgttc tacccggaga acccggcgga tcgcttccgg
     4141 atcgggaagg gctacttcct gttgctggag aagcctgtgg tgttgacgcg cgagggcaaa
     4201 gcggcggaca cctcgaagcc ggcgactctg aacctgacgg cc
//
LOCUS       DAOSCV010000321         4239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13156_length_4239_cov_3.632648, whole genome shotgun sequence.
ACCESSION   DAOSCV010000321 DAOSCV010000000
VERSION     DAOSCV010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13156_length_4239_cov_3.632648"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLY56_08910"
     CDS             complement(<1..1166)
                     /locus_tag="PLY56_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21641.1"
                     /translation="MRRAAKVGLLPFYLALYDSALPEARHTGERFVRQIVGELERLGL
                     EVQTAPLCRVEAEFDAAVRGFEDAGVDAILTLHVAYSPSLESAPVLARTSLPIIVVDT
                     TPEYAFGPDQSPDAIMANHGIHGVQDLCNMLLRLKKPFRVEAGHWEHSDLLHRVARWA
                     NAACLVSALRRARVGRIGRPFPGMGDFAVPPERLRATIGVETVPCEPETIRGRLDEIT
                     EEEVDAEVAGDLAACETEDLALGAHREAVRAGLAVRRWIESEKLTAFTVNFLDVDRAS
                     GLPTVPFLEASKAMARGIGYAGEGDVLTAALVGALATRYPETTFTEMFCPDWAGNRVF
                     LSHMGEVNLNLVAGKPKLVVRPFPWTDADDPVVAVGRLKAGHALWVDLAPGPDE"
     gene            complement(1281..2342)
                     /locus_tag="PLY56_08915"
     CDS             complement(1281..2342)
                     /locus_tag="PLY56_08915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ21642.1"
                     /translation="MSQLFEVKEIDRKFYAERVLDFLPERIIDIHTHVWLERFRSAVM
                     TAPRRTVTWPSRVASSNSIEDHLESYRLLFPGKHVLPMIFGNAVDPVDDIEAGNAYIR
                     RCAAEYSLPALLFATPRWSSAELEERVLAGGFLGVKVYLSLAEPYIPAAEIRIFDFLP
                     PHQLEVLDRHGWIVMLHLPRPKRLRDPVNLAQMLQIEERYPNVKVIIAHVGRAYCPED
                     VGNAFEVLRRTRRMRFDISANTNAWVFERLIEAVGPKRILFGSDLPIVRMRMRRICES
                     GTYINLVPRGLYGDVSGDPNMREVDGEEAERLSFFMYEEIDAFRSAAEVTGLTREEVG
                     DIFWGNGARLLREVGFEVA"
     gene            complement(2339..3127)
                     /locus_tag="PLY56_08920"
     CDS             complement(2339..3127)
                     /locus_tag="PLY56_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21643.1"
                     /translation="MRRMLADGNAVIIACDHGEFDGPIPGMVDLRETVAKIRPEVDGV
                     LLSPGMIRHVGEYFARKGAPLVVGRLNWDTVYCFHWGYNEAPAVEAFSAEEAAALGVD
                     VALISLTLKTGSEERDARNVALFRKLAADCHRLGLPVIGEYFPAHSDTQSPEAMHQEV
                     KIGCRILAELGADMIKTFHTRAFPEVVAGCPIPIFALGAAKLPTPLDALRLAHREVSD
                     GARGVVFGRNALQVEDPRRFQSALLRVVKEGMEPEVALAEAGLQ"
     gene            complement(3165..>4239)
                     /locus_tag="PLY56_08925"
     CDS             complement(3165..>4239)
                     /locus_tag="PLY56_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21644.1"
                     /translation="LKLKDNASEHQRWIYLSVPRQQVSAAEVVFDLDQARFSVPLRQV
                     LERRMIARAFENGLQFTLFPYRRQIDMPVHLGRKEVSFTARVWPEIPTEQYHLRLTTV
                     SGKVFRSRPLLLPGAGAQPKRPLRVYSQREKRPLEVQVSSDRVPVLDYEFSPERGAVL
                     LTPAGRPFWASLGGFTSTTTGRGALNGLFTGIYPAQATRTAPEWVEGEGGSYLRFDGT
                     GTYLELPREALPRRGAFTLELEVRPATDRDQCLLTTRTVGHQNGLGLSIVGGKLHATF
                     RAEDWSTVSCPTELSVPAGAWSTIRVRHDFEKLTIAVNGRDESFPVTMPAYNIGFTLI
                     GEGWKGSWFAGDLRRLRVAHHAE"
BASE COUNT          737 a         1493 c         1330 g          679 t
ORIGIN      
        1 ctctcgtccg ggcccggggc gagatccacc cagagcgcgt gccctgcctt cagccggccg
       61 acggcaacga cggggtcgtc ggcatcagtc cacgggaagg ggcgaacgac gagcttcggc
      121 ttgcctgcga ccaggttgag gttcacctcg cccatgtggc tgaggaagac gcggttgccc
      181 gcccaatcgg ggcagaacat ctcggtgaag gtcgtttccg gataccgggt ggcaagtgcg
      241 ccgacgagcg ccgcggtgag cacgtcgcct tcccccgcgt agccgatgcc ccgggccatc
      301 gccttgcttg cctccaggaa cggcacggtt ggcaagccgg aggcacgatc cacatccagg
      361 aagttgacgg tgaacgccgt tagcttctcg ctctcgatcc accggcgtac ggccaggccc
      421 gcccgcaccg cttcacgatg ggcgcccaag gccaggtcct ccgtctcgca cgccgccagg
      481 tccccggcca cctcggcatc cacctcttcc tcggtgatct cgtcgaggcg cccgcggatc
      541 gtctccggct cgcatggcac ggtctcgacg ccaatcgtcg cgcgcaggcg ctcgggaggc
      601 acggcgaagt cacccatccc cgggaaaggc cggccaatcc ggccaacccg cgcccggcgc
      661 agagcggaaa ccaggcacgc cgcgtttgcc caccgcgcca ccctgtgcag gagatcggaa
      721 tgctcccaat ggcccgcctc cacccggaac ggctttttca ggcgcaggag catgttgcag
      781 aggtcctgca cgccatggat gccgtggttc gccatgatcg cgtccggcga ttggtccggc
      841 ccgaaggcat actccggagt ggtgtcgacg acgatgatcg gcagcgaagt gcgcgccagg
      901 accggcgccg actccagcga gggtgaatag gcgacgtgga gcgtgagaat ggcatccacg
      961 cccgcgtcct cgaagccacg gacggcggcg tcgaactccg cctccactcg gcagagggga
     1021 gccgtttgga cctccagacc aagccgctcc aactcgccga cgatctgccg gacgaaccgc
     1081 tcgcccgtgt gccgggcttc cggaagcgcg ctatcgtaaa gcgccaggta gaacggcaga
     1141 agacccactt tggctgcgcg tcgcatcgcc gcttgctcct ctcacaggta tccaacaggc
     1201 ggctggccgg ccgctatcac tgccggaaca ccatcgccct tccccggaag cataccctat
     1261 tccgcggacc gcgccggggg ttatgcgacc tcgaagccaa cctcccggag caggcgcgcg
     1321 ccattgcccc agaagatatc cccgacctct tcccgagtga gcccggtcac ctcggcggca
     1381 ctgcggaagg catcgatctc ctcgtacatg aagaaggaga ggcgctcggc ttcctcacca
     1441 tccacctcgc gcatgttcgg gtcgcccgag acgtcgccat aaagccctct tggcaccagg
     1501 ttgatgtagg taccggattc gcagatccgg cgcatgcgca tccgcacgat cggcagatcg
     1561 ctcccgaaga ggatccgttt cggtccaacc gcctcgatca ggcgctcgaa cacccaggcg
     1621 ttcgtgttcg cgctgatgtc gaagcgcatg cgccgggtgc ggcgcaacac ctcgaacgcg
     1681 ttgcccacgt cctcggggca gtaagcccgg cccacgtggg cgatgatcac cttcacgttc
     1741 ggatagcgct cctcgatctg cagcatctgc gccaggttca ccgggtcgcg caggcgcttc
     1801 ggacgcggca ggtggagcat cacgatccag ccgtgccggt cgagcacctc cagctggtgg
     1861 ggcgggagga aatcgaagat ccggatctcc gccgccggga tgtagggctc ggcgaggctg
     1921 agatagacct tgacccccag gaatccgcca gcgaggacgc gctcttccag ctccgcgctg
     1981 ctccagcgtg gcgtcgcaaa gaggagcgcc ggcagggagt attccgccgc gcacctccgg
     2041 atgtaggcgt tccccgcctc aatgtcatca acgggatcga ccgcgttccc gaagatcatc
     2101 ggcaggacgt gctttcccgg aaaaaggagc cgatagctct ccaggtggtc ttcaatcgag
     2161 ttgctgctag ccacccggga gggccaggtc accgtccgcc ggggggcggt catgacagcg
     2221 gagcggaagc gttccagcca gacgtgcgta tggatgtcga tgatgcgctc tggaaggaaa
     2281 tcgagcacgc gctccgcata gaacttccgg tcaatctcct tgacctcaaa cagctggctc
     2341 actgcagacc tgcctccgca agcgccacct cgggctccat gccctccttc accacccgca
     2401 agagcgcgga ctggaagcgc cgcgggtcct ccacctgcag ggcgttgcgc ccgaaaacca
     2461 cccccctggc gccatcgctc acctcgcgat gcgccaggcg cagcgcatcg agtggcgtgg
     2521 gcagcttggc ggcccccagc gcgaagatgg gaatcgggca gccggccacc acctcgggga
     2581 aggcgcgcgt gtggaacgtc ttgatcatat ccgcgcccag ctccgcgaga atgcggcatc
     2641 cgatcttgac ttcctgatgc atcgcctcgg ggctctgcgt atcgctgtgc gccgggaaat
     2701 actcgccgat gacgggcagg ccaaggcgat ggcaatcggc ggccagtttc cggaagagtg
     2761 ccacgttgcg cgcgtcgcgt tcctcgctcc ccgtcttcag cgtgagcgag atgagcgcca
     2821 cgtccacccc gagtgcggcc gcctcctccg cggaaaacgc ctccaccgcc ggcgcttcgt
     2881 tatagcccca atggaagcag tagaccgtgt cccagttcag ccggccaacg acgagcggag
     2941 cgcccttcct cgcgaaatac tctcccacgt gacggatcat ccccgggctg agcaacaccc
     3001 cgtccacttc cggacggatc tttgccaccg tctcgcgcag atccaccatt ccggggatgg
     3061 gaccgtcgaa ctcgccgtgg tcacacgcga tgatcacggc gttgccgtcg gccagcatcc
     3121 gtcgcatttt gatctctacc gcattcatcg attgtcccct taccctactc cgcgtggtgc
     3181 gcgacccgca ggcggcgcaa gtcgcccgcg aaccaggacc ccttccaccc ctcgccgatc
     3241 agggtgaagc cgatattgta ggccggcatg gtcacaggga aactctcatc cctgccattc
     3301 acggcgattg tcagcttctc gaaatcgtgg cgcacacgga tggttgacca ggctccggcc
     3361 ggcacagaca gctccgtggg gcagctcacc gtgctccaat cctcggcccg gaaggtggcg
     3421 tgcagcttgc ccccgacgat gctcagcccc aggccgtttt ggtgacccac agtgcgcgtg
     3481 gtgagcaggc actggtctct gtccgtcgcc ggcctcactt ctagctccag ggtgaacgcc
     3541 ccgcgccgcg gcagcgcctc gcgcggcagc tccaggtagg tgccagtgcc atcaaaccgc
     3601 aggtaggagc caccctcgcc ctcaacccat tccggcgcgg tgcgagtggc ctgcgccggg
     3661 tagatgccgg tgaacagccc gttgagcgcg ccccgccccg tcgtggtgct ggtgaaccca
     3721 cccaggctcg cccagaaggg ccggccggcc ggcgtgagca acaccgcccc gcgctccggc
     3781 gaaaactcgt agtcgagaac cggaacgcgg tcgctagaaa cctgcacttc cagggggcgc
     3841 ttttcgcgct gcgagtagac gcgcagcggc cgcttcggtt gggcgcccgc gcccggtaga
     3901 agcagcggcc gcgagcggaa caccttgccg ctcaccgtgg tcaggcgcag gtgatactgc
     3961 tccgtgggta tctccggcca gacgcgcgcc gtgaacgaga cctccttgcg gcccaggtgg
     4021 accggcatat cgatctggcg ccgataaggg aaaagggtaa actgcaggcc gttctcgaaa
     4081 gcccgcgcga tcatccggcg ctccagcacc tggcgcaacg gcacggagaa gcgcgcctgg
     4141 tcgaggtcaa acaccacttc cgccgcgctc acctgctgtc ggggcacgga gaggtagatc
     4201 cagcgctgat gctcggaggc gttgtctttg agcttcagg
//
LOCUS       DAOSCV010000322         4238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13160_length_4238_cov_3.759503, whole genome shotgun sequence.
ACCESSION   DAOSCV010000322 DAOSCV010000000
VERSION     DAOSCV010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13160_length_4238_cov_3.759503"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLY56_08930"
     CDS             complement(<1..860)
                     /locus_tag="PLY56_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21645.1"
                     /translation="MNARIIVWAVIIALVGITAGTKGAEEGTPADVVHRVGPTGFSVQ
                     PVEGVKAGVVKAGEQEVFSVSSANARAVFAVDLGWMSVEPNRAYRARCSFRAPGLDAP
                     ASARLMIREHEAKGVRPITPYHVTPVARRPVTPGTPEAMFTRELTFTTGPKTRTLSGA
                     LVIAELKGEILLTSFELVDAARQEEAARQQAQAEYEKLMAEIRAKADARVPLLPRPLV
                     FSRSQMKYGLELNYYHAWNDRPLLVNRAYRVPRKYVTPLPGYKRTLQEVAKYDLDGLA
                     FFPETKDRMG"
     gene            1106..1705
                     /locus_tag="PLY56_08935"
     CDS             1106..1705
                     /locus_tag="PLY56_08935"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOJ21646.1"
                     /translation="MAQPKHTEQELLLVDYENVQNIELDRLGDHYRIIIFVGANQKSI
                     PIDLVTRTQPLGRRCEWLKVDGNGSNALDFFIACHLGRVLEKSPQTHCTILSKDKGFD
                     PLLRHLNKIGLKCKRISSLLELDPKPAGPEEPNYQRVVELLSKSAKKSRPRKLTTLSQ
                     HISSMFQKNIAQPEVDRIIDILFANKMISESNGAITYEF"
     gene            complement(1827..3284)
                     /locus_tag="PLY56_08940"
     CDS             complement(1827..3284)
                     /locus_tag="PLY56_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21647.1"
                     /translation="MSSKIAIGWAGRDITPDRPVSLRGLFNLRIATRVRDPLTLTALA
                     LESGGDQAILVSIDACAVDQEVLDRSRALLRERLPEFDARKLVASATHTHTAPFAGGG
                     IALQKDVEYLDDIRARYPNYMTTAEYTDLLVDALVSAACEAWQNRREGSVGWGYSYAV
                     VGENRRVRYFDGRAMMYGKTSEPDFSHIEGHVDHGVNLLFTYDPAGALTGVLVNVACP
                     SQANEGGQDYVSADFWHDTREKLRELFGTGLFILPQCSAAGDQTPHRLIQTRAEERML
                     ALKYGEGTYHRRDNSALRRDIARRIGEAVADAEPAVRKDLRDEVVLKHVQRALDLPHW
                     DVTDKEYAALQEEIGRLRAQLETVGDSDRLNSTYTALRSRIAWCQRAVNRYHEPLKSV
                     PVEINILRLGDIAFVTSPFEYYLDFGDRIKGRSPALQTFVVQLAGGGTYLPTERAAEG
                     LSYGAVPASCRVSPAGGQVIVDEAVSTLNTLFAEE"
     gene            complement(3315..4103)
                     /locus_tag="PLY56_08945"
     CDS             complement(3315..4103)
                     /locus_tag="PLY56_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21648.1"
                     /translation="MKLCMMSYTMARQGVGVEEIVRCAEELGLEGIDWVSTYGRDARE
                     LRALSDAAGLTVACHTFFLRSFPEGDGWLDEAKQGIDDAVALGAPVVMIPTGTRPEMD
                     RSEFRRRWIDALAEIMPFAREAGVILTVENFPGAASAFVTAADFYEAQRQVPGLKLTF
                     DNGNAASGEDPVASFRQCAADVVHIHLKDWTVRSTPAEGYRQMLDGRYYRPALIGEGD
                     MPTAECWQAFREAGYGGYINIEYEGNEIPAAEAMRRAVSYLRSL"
BASE COUNT          749 a         1475 c         1287 g          727 t
ORIGIN      
        1 atgcccatcc gatccttggt ctccgggaag aaggccaggc cgtcgaggtc gtacttcgct
       61 acttcctgaa gggtgcgctt atatccaggc aagggggtga cgtacttgcg cggcacgcga
      121 taggcgcggt tgaccaggag cggacggtcg ttccaggcgt ggtagtagtt gagctccagg
      181 ccatacttca tctgcgaccg gctgaagacg agcggccggg gaaggagagg cacgcgcgcg
      241 tctgccttgg cgcggatctc cgccatcagc ttctcatact ccgcctgcgc ctgctggcgc
      301 gccgcttcct cttgccgagc cgcgtccacc agctcgaagc tcgtcagaag aatctccccc
      361 ttcagttcgg cgatcaccag ggcaccgctc agggtgcgcg tcttcggccc ggtggtaaag
      421 gtgagctcgc gcgtgaacat ggcctccggg gtgcccggcg tcaccgggcg ccgagccacg
      481 ggggtgacgt ggtaaggcgt aattgggcgg acgcccttcg cctcgtgctc gcggatcatc
      541 aggcgcgcgc tcgccggcgc atccaatccg ggcgcgcgga agctacaccg cgcgcgatag
      601 gcacggttcg gctccacgct catccatccc agatcaaccg caaacacggc gcgcgcgttg
      661 gcgcttgaga ccgagaagac ctcctgctcg ccggctttca ccacgcccgc tttcacgcct
      721 tccacgggct gcaccgagaa gcccgtgggc ccgacgcgat gcactacgtc cgccggcgtg
      781 ccctcctctg cccccttggt cccagccgtt atccccacca gggcgatgat aaccgcccac
      841 acgattatcc ttgcgttcat ccctctcctg ccaacatcgc gattcgcccg ttccggcagc
      901 tcgcctgctt agtgagcggg ctgctcctgc attcgccgct cgcggggaga accccttgcc
      961 ccaacgcgcc gccggtgtgt gggcagattc cggagttgtt gacgcgctac cggcggggtg
     1021 ctataatcca aacagaacaa gcgttcgcgt gccacattgc tccaacctgg cacgcgctcc
     1081 gcatcctgat gagtggaagc gagacgtggc acagccaaag cacacggaac aggaattact
     1141 cctcgttgac tacgagaacg tgcagaatat cgaactagac cgcctcggtg accattaccg
     1201 catcatcatc ttcgtcggcg ccaaccagaa gagcatcccc atcgatctcg tcacccgcac
     1261 gcagccgttg ggccgccgct gcgaatggct gaaagtggat ggcaacggaa gcaacgcgct
     1321 cgatttcttt attgcgtgtc atctgggccg cgtcctggag aagtctccgc agacgcactg
     1381 cacgattctc tcgaaagaca aggggtttga tcccctcctc cgccacctca ataagattgg
     1441 cctgaaatgc aagcggatta gcagcctgct tgagcttgac cccaaaccgg caggaccgga
     1501 ggagcccaac taccaacgcg tcgtcgagct gctcagcaag tcggccaaga aatcacgacc
     1561 acgaaaactg acgacgctgt ctcaacacat ctcctcgatg ttccagaaga acatcgctca
     1621 accggaggtc gaccgtatca tcgacatcct ctttgccaac aagatgatct ccgagagcaa
     1681 cggggcgatc acctacgagt tctgacgggc gcgggcagcg agccggggca agtcttgagg
     1741 aacgggcgcg cggttgcttc tccccaaaaa gcggaatgcg cgcccgttta ggcgcgcact
     1801 ccgctggtca tctctgaggc tctcgcctac tcttcggcaa acagcgtgtt gagcgtagag
     1861 accgcctcat ccacaatcac ctggccgccg gccggcgaaa cacggcagct ggccggcacg
     1921 gcgccatagc tgagcccctc cgcagcgcgc tccgtcggca ggtaagtacc gccgcccgcg
     1981 agttggacca cgaaggtctg tagcgccggg ctgcgcccct tgatgcgatc gccgaaatcg
     2041 aggtagtact caaagggcga ggtgacgaat gcgatatcac ccaagcgcag gatgttgatc
     2101 tctaccggca cgctcttcag gggctcgtga tagcggttca cggcgcgctg acaccacgcg
     2161 attcgagagc ggagcgccgt gtaggtgctg ttcaggcggt cactatcacc cacggtctca
     2221 agctgcgcgc ggagccggcc gatctcctcc tggagcgcgg catactcctt gtccgtgacg
     2281 tcccaatgcg ggagatcgag cgcgcgctgc acgtgcttta gcaccacctc atcgcgcagg
     2341 tccttgcgca cggccggctc ggcgtcggca acggcctcac cgatccggcg cgcgatatcg
     2401 cggcgcaggg cggaattgtc gcgccggtga taggtgccct cgccgtactt gagcgccagc
     2461 atccgctcct cggcgcgggt ctgaatgagg cggtgcggcg tctggtcgcc cgccgctgag
     2521 cattggggca ggatgaagag accggtgccg aaaagctcgc gcagcttctc gcgcgtgtcg
     2581 tgccagaagt cggccgagac gtagtcctgc ccgccctcgt tcgcctggga ggggcaggcg
     2641 acgttgacca ggacgccggt cagcgcgccc gcggggtcgt aggtgaacag caggttcacg
     2701 ccgtgatcca cgtgcccctc gatatgcgag aagtcgggct cgctcgtctt gccatacatc
     2761 atggcacggc cgtcgaagta gcgcacacgc cggttctcgc ccaccaccgc gtacgaatag
     2821 ccccagccaa cagagccctc gcgccggttc tgccacgcct cgcacgccgc cgacacgagc
     2881 gcatccacca gcaggtcggt gtattcggcg gtggtcatat agttggggta gcgggcgcgg
     2941 atgtcgtcga ggtactccac gtccttctgc agggcaatgc cgccgccggc aaagggcgcg
     3001 gtgtgagtgt gcgtcgctga ggcgaccagc ttgcgcgcgt caaactccgg gagccgttcc
     3061 cgcaagagcg cccgggagcg gtccagaacc tcttgatcca cggcgcaggc atcgatggag
     3121 acgaggatcg cctggtcccc cccgctctcg agggcaagcg ccgtcagcgt cagcgggtcg
     3181 cggacgcgcg tcgcgatccg caggttgaag agcccccgca acgacacggg gcgatccggc
     3241 gtaatgtccc ttccggccca accgatagcg atcttggagc tcatcttgtc cctcctgtgc
     3301 cctctccggc ttgatcacag gctgcgcagg taggagacgg cgcggcgcat cgcctcggcg
     3361 gccgggatct cgttcccctc gtactcgatg ttgatatagc cgccgtatcc cgcctcccga
     3421 aacgcctgcc aacattccgc cgtgggcatg tcgccctcgc ctatcagcgc cgggcggtag
     3481 tagcgcccgt ccagcatctg gcgatacccc tcggccggcg tgctccggac cgtccaatcc
     3541 ttcaagtgga tatgcaccac gtccgcggcg cactgccgga acgaggcgac agggtcctcg
     3601 ccactcgccg cgttcccatt atcgaaggtt agcttcaggc ccggcacctg ccgttgcgcc
     3661 tcatagaaat cggccgccgt gacaaacgcg ctggcggcac ccgggaagtt ctccaccgtc
     3721 aggatgacgc ctgcttcccg cgcgaacggc atgatctccg ccagggcgtc gatccagcgc
     3781 cgacggaact cggagcggtc catctccggg cgcgtgccgg tcgggatcat taccacgggc
     3841 gcgccaaggg ccacggcgtc gtcgatcccc tgcttcgcct catccagcca cccgtcgcct
     3901 tccgggaaag aacggagaaa gaaggtgtgg caggcgaccg taagccccgc cgcgtcgctc
     3961 agcgccctca gctctcgcgc gtcacggcca tacgtggaga cccagtcgat cccttccagg
     4021 cccagctcct cggcgcaccg gacgatctcc tcaacaccaa ccccctgccg ggccatcgtg
     4081 taggacatca tgcacagctt catcgcgttc cccatccctt cacccgcgcg cgcccccggc
     4141 acatcgccct catggcgctg cccgggcaca gccggcgtgc caacgaccaa tgcgccggtc
     4201 ctcactcagc cagccggtgt cttccaccag ttcgccag
//
LOCUS       DAOSCV010000323         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13196_length_4230_cov_3.834012, whole genome shotgun sequence.
ACCESSION   DAOSCV010000323 DAOSCV010000000
VERSION     DAOSCV010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13196_length_4230_cov_3.834012"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLY56_08950"
     CDS             complement(<1..661)
                     /locus_tag="PLY56_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21649.1"
                     /translation="MSGRLQLDGAAPPRARRLLVYRYWGWSLGITTALLILYRTHPYY
                     VRILGSPNAPLPESRVQLALLLIYLAYLFVILSAPFRGDISKSRILLVANLLESLPGR
                     LRQYAWGPQPAYEGLVPLTPETRTALLSLVVKAFFLPIMLDFFLEHLAGLQQAGYRWM
                     SSIGPPTLHMGLDVVIQLLFVVDTGIFVFGYAVESERLGSTIRSVEPTLLGWVVTLAC
                     YP"
     gene            complement(666..1622)
                     /gene="era"
                     /locus_tag="PLY56_08955"
     CDS             complement(666..1622)
                     /gene="era"
                     /locus_tag="PLY56_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ21650.1"
                     /translation="MEHTVESAMAMEGERFRSGFVLLWGRPNVGKSTLLNAILGRKVA
                     IVTPKPQTTRNRIAGVLTTKTAQFVFVDTPGIHAPHDALGEYMLATAREALPDADVVL
                     FVVDASLPPREADREALRLLQSIETPTLLVLNKIDLITEEILAERREEYAALGSFSDT
                     ITVSAVQEAGTQGLLQRTEELLPIGPMYYPAEMVSDHPDSFLIAELVREQALLQTQKE
                     VPYSLAVIVEEMAPRPEDLIYVRAVLYVERDSQKKILIGQGGNKLREIGKAARAQAEA
                     LLGKRIFLDLWVKVREHWRDDESVLARFGYPIPKKRKKRKAR"
     gene            complement(1870..2088)
                     /locus_tag="PLY56_08960"
     CDS             complement(1870..2088)
                     /locus_tag="PLY56_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21651.1"
                     /translation="MDDRGRAKEYLVDRLKRDGVISGTPEALAADAGFTARAMEEALA
                     ELVAENRIQPFQDDEGNLEYQWKEYQLF"
     gene            complement(2188..3675)
                     /gene="cobA"
                     /locus_tag="PLY56_08965"
     CDS             complement(2188..3675)
                     /gene="cobA"
                     /locus_tag="PLY56_08965"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOJ21652.1"
                     /translation="MRENGFVYLVGAGPGDPGLITCRGLECIQTADCLVYDRLASQRL
                     LAHARPDAERVYVGKEAGRHALPQEEINLLLIARAREGKLVCRLKGGDPYVFGRGGEE
                     AAALHAAGIPFAVVPGVTSAIAVPAYAGIPVTHRGVASSFAVITGHEDPSKPHSSLRW
                     EHLSTAADTLVFLMGVENLPQIVAKLIAHGRPESTPVAVIHQGTTPQQQTVVGTLADI
                     AARVQESGLRPPAVTVVGEVVCLREPLRWFDSQPLFGRRIVVTRARAQASDLAAHLER
                     LGAEVVEFPTIRIVPLEWDEEVAGYDWLLFTSANGVACFFDRIWSRGGDVRAIGPARL
                     GAIGPATAAALEARGLRVDYVPPRFVAEEVADHFPEDPRGKRILIPTARQARPVLAER
                     LTARGATVRVLPVYETVPDAAAADEIAAQIRAGTVDLVTFTSSSTVRHFVDALGAALA
                     AQVPAAVIGPLTAETARAAGLKVAIKAGEHTIPGLVDAVVAHFSA"
     gene            complement(3668..>4230)
                     /gene="hemC"
                     /locus_tag="PLY56_08970"
     CDS             complement(3668..>4230)
                     /gene="hemC"
                     /locus_tag="PLY56_08970"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOJ21653.1"
                     /translation="LRRQAQLRHHRPDLRFCDLRGNVDTRLRKLDEGAMDAIILAAAG
                     LIRLGLAERISARLPEEISLPAVCQGALAIECRADDESTLTLLQPLEDSETRCCVTAE
                     RALLRALGGGCAVPVGGLARIEGTALVLQGAIAAPDGTRLVREIQTGSPDDPEGLGAA
                     LGARLLATGGQEILQEMHSQRGIQDA"
BASE COUNT          724 a         1398 c         1433 g          675 t
ORIGIN      
        1 agggatagca cgccagggtc acgacccaac cgaggagagt tggctccacc gagcggatgg
       61 tggaaccgag acgctcggac tccaccgcgt agccgaagac gaagatcccc gtatcgacca
      121 cgaaaagcag ctgtattacc acgtccagcc ccatgtggag tgtcgggggc ccgatgctgc
      181 tcatccagcg gtaccccgcc tgttgcagcc ccgccaggtg ctcgagaaag aagtcgagca
      241 tgatcggcag gaagaacgcc ttcaccacca ggctgaggag cgccgtccgg gtctcaggag
      301 tgagcggtac cagcccctca taggctggtt gtgggcccca cgcatactgg cgcaggcgtc
      361 cgggaaggct ctccagcagg ttggcgacca gcaagatccg gctcttggag atatcgcccc
      421 ggaagggcgc gctgaggata acgaaaaggt aggccaggta gatcaggagt agcgcgagct
      481 ggacgcgcga ctcaggcaac ggcgcgttcg gggatccgag aatccgcacg tagtagggat
      541 gggtgcggta aaggatcagg agcgccgtcg tgatgcccag cgaccacccc cagtagcggt
      601 agacgagcaa ccttctcgcg cgcggtggcg cggcgccgtc taactggagg cggccgctca
      661 tagccttagc gcgcctttcg tttcttgcgc tttttgggaa tcgggtagcc aaagcgcgcc
      721 agcacggact catcatcgcg ccagtgctcg cgcaccttca cccacagatc caggaagatc
      781 cgcttgccga gcagagcctc cgcctgagcc cgcgccgcct tcccaatctc gcgcagcttg
      841 ttgcccccct ggccgatcag gatcttcttc tggctgtcgc gctccacgta gagcaccgcg
      901 cgcacataga tcaagtcctc tgggcgcggc gccatctcct ccacgatgac tgccagcgag
      961 taggggacct ccttctgcgt ctgcaacagc gcctgctcgc gcaccaattc ggcgatcagg
     1021 aaagaatcgg gatgatccga caccatctcg gccggatagt acatcggccc gatggggagg
     1081 agttcctccg tgcgctgtag cagcccctgc gtgcccgcct cctgaacggc tgagaccgtg
     1141 attgtgtcgc tgaagctgcc aagagcagca tactcttccc ggcgctcggc cagtatctct
     1201 tccgtgatga ggtcgatctt attcaggacg agcagcgtcg gggtttcgat cgactggagc
     1261 agccgcagcg cttctcggtc ggcctcccgc gggggcaagg atgcatcgac gacaaagagc
     1321 accacgtccg catcggggag tgcttcgcgc gcggtggcga gcatatactc tcccagggca
     1381 tcatgcggcg catggatccc cggggtatcg acaaagacga attgcgccgt cttggtcgtg
     1441 agcacgcccg cgatgcggtt gcgcgtggtc tgtggcttcg gggtgacgat cgccaccttc
     1501 cgcccgagaa tggcattgag cagcgtggat tttccgacgt tgggccgccc ccagaggagt
     1561 acaaagccag atcgaaagcg ttcgccctcc atcgccatcg ccgattccac tgtgtgttcc
     1621 atgcttccgc attataccgg agtcctggcg atgggtcaag ctggcggtcg gtggtatcgc
     1681 gcggggttga ctccccgcgc tgcttgtgag cgaagtcgtg tcgggctagg ccgggttaca
     1741 ctcccgacgc aagcgcagcg gcgtgggatg ggaacggcgc attcgcgcgg agacaaatca
     1801 ccgtgcgctg ggtagcccgc cctcgccatc cgtgcccgtc cccgtcatcc gtgggatcgc
     1861 cgccggcgct cagaacagct gatactcctt ccactggtat tccagattgc cctcgtcatc
     1921 ctggaaaggc tggattctgt tctcggcgac gagctcggcg agtgcttcct ccatggcgcg
     1981 cgctgtgaac ccggcgtcgg cggccagcgc ctcgggcgtg ccgctgatta ccccgtcgcg
     2041 tttcagtcgg tcaaccagat actcctttgc tctgccccgg tcatccatcg cccacctccg
     2101 atcgggatct accccaccgg ctggatggca gaaacccggg ttggggagcg ccacgccggc
     2161 ggatcgctgg caatggatcc cgcctgacta tgccgaaaag tgggctacca ccgcatcaac
     2221 caggccgggg atggtgtgct cgccggcttt gatggcgacc ttgaggccgg ccgcgcgcgc
     2281 cgtctcggcg gtgagcggcc cgatgacggc ggcgggaacc tgggccgcga gggcagcgcc
     2341 cagggcatcg acaaaatgac gcactgtcga ggagctcgtg aaggtgacca agtccacggt
     2401 gccggcgcgg atctgggctg cgatctcatc cgcggccgcg gcatcgggca ctgtttcgta
     2461 aaccgggagc acgcgcaccg tggcgccgcg cgcggtgagg cgctcggcaa gcacggggcg
     2521 ggcctggcgc gctgtgggga tgagaatccg cttgccacgc ggatcctccg ggaagtggtc
     2581 ggccacctcc tcggcgacga agcggggtgg cacgtagtcc acgcgcaacc cgcgcgcttc
     2641 gagcgccgcc gccgtcgcgg ggccgatggc gcccaggcgc gccggaccga tggcgcgcac
     2701 atcaccgccg cgagaccaga tccggtcgaa gaagcaggcg acaccgttgg cgctggtgaa
     2761 gaggagccaa tcgtatccgg ccacctcctc gtcccactcc aggggaacga tgcggatggt
     2821 cggaaactcc accacctcgg cgccgaggcg ctccaggtgc gccgccagat cggaggcctg
     2881 cgcccgcgcc cgagtcacca caatccgcct gccgaaaagc ggctgcgagt cgaaccagcg
     2941 gagaggctcg cgcaggcaga ccacctcgcc aaccaccgtc acggcgggcg ggcggaggcc
     3001 gctctcttgc acccgggcgg cgatatcggc gagggtgccg accaccgtct gctgttgcgg
     3061 cgtggtgccc tggtggatca ccgcgacggg tgtgctctcg gggcgaccgt gcgcgatcag
     3121 cttcgcgacg atctgaggca ggttctcgac gcccatcaag aagacgagcg tgtctgcggc
     3181 cgtggagagg tgctcccagc ggagggaaga gtgtggtttg gatggatcct catggccggt
     3241 gatcacggcg aagctggagg ccaccccgcg gtgggttacc ggaatccccg cgtaagccgg
     3301 cacggcgatg gcggaggtga cccccggcac cacagcaaag gggatgcctg ccgcgtggag
     3361 tgccgcggcc tcctcgccac cgcgaccaaa gacgtagggg tcgccgccct tcaaccggca
     3421 gaccagcttg ccctctctcg cgcgcgcgat gagaagcagg ttgatctcct cctggggcag
     3481 ggcatgacgc ccggcctcct tgcccacgta aacgcgctcg gcatccgggc gcgcgtgggc
     3541 tagaagtcgc tggctggcca gacggtcgta gaccaggcaa tcggcggtct ggatgcactc
     3601 caggccgcgg caggtgatca gccccggatc gcccgggccg gcgccgacga gatagacgaa
     3661 gccgttctca cgcatcctgg attcccctct gggaatgcat ctcctgaagg atctcctggc
     3721 cgccggtcgc gaggaggcgc gcgcccaagg cagcaccgag tccttccgga tcgtccggcg
     3781 atccagtctg gatctcgcga accagccggg ttccgtccgg ggcggcgatg gcaccctgga
     3841 ggacgagcgc cgttccctcg atgcgcgcga ggccgcccac cggcacggcg cagccaccgc
     3901 cgagcgcccg gagaagagcg cgctccgcgg tgacgcagca gcgggtctca gagtcctcga
     3961 ggggctgcaa cagcgtgagc gttgactcgt catcggcgcg gcattcgatg gcgagtgctc
     4021 cctggcacac ggcgggaagg gagatctcct cgggcaaccg tgccgagatc cgttccgcca
     4081 gccccaggcg gatcaggcct gccgccgcga ggatgatggc atccatcgct ccctcatcca
     4141 gcttgcgcag gcgcgtatcc acgttgccgc ggaggtcgca gaagcggagg tcggggcggt
     4201 ggtggcgaag ctgggcctga cggcggaggc
//
LOCUS       DAOSCV010000324         4218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13240_length_4218_cov_3.419890, whole genome shotgun sequence.
ACCESSION   DAOSCV010000324 DAOSCV010000000
VERSION     DAOSCV010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13240_length_4218_cov_3.419890"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="PLY56_08975"
     CDS             <1..1777
                     /locus_tag="PLY56_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21654.1"
                     /translation="VVLLFLLVSWSLVWRRFSPRLGRTLALSRSELVLIYLMATVGTA
                     LAGECWAVYVVPALLNTAAYQAETARPEWAAWLGDSPSWFAVQDPEVIRQAHRGNASL
                     YRVSVLRALAVPVLAWSVLMLALALFTQCLAQLVRRQWIERERLSFPLIHLPMTLVAF
                     DAPRPWWRTRLFWAGVTVAGIIQLINGLNYLVPNVPYIPVKWIQFPPASDRPWSGLGR
                     FQIAFYPFVIAIGFMVPLEVSFSMWFFFWLGRVQDMVVTILGHRSVGGHAPSLPPYHL
                     HQAAGAFLVLGAAFLWRSRHEIAGIWRKAAEPTGRRAGWALIGSVGVILAWAYLARIP
                     LPLATLFLVIYGLFAIVLGRLIAESGSAMSMSPLGVHETAALLTNLGTQTQQSLVAFG
                     WWHHLGERLSDNLLPHQVTGMRMAEDVSAGRRVQLFLAVGAVVGVLTGIWALLHIYFK
                     YGIMSAEVRDWPARWAPQYAYGLIQNWLRGQTEIPREKLIAFVAGGGVSALLLWLRQA
                     YLGWPLHPIGYAVAGHFAMQQLWCPFFVAWLVKLLTMRFGGIRTYRALLPFFLGAILG
                     DYLVPMGWAVVGLLSGQQMYLSYPH"
     gene            complement(1787..3862)
                     /locus_tag="PLY56_08980"
     CDS             complement(1787..3862)
                     /locus_tag="PLY56_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ21655.1"
                     /translation="MRFAMAAAVNVEDLVGILGQVPLAVCALEIPSLRILAANQALAS
                     LAAAGNPAEIVGVTLSEEVQRGVLSDLLPPLREAAAAGGNLPPREVHAQLGGESRDLL
                     VQCHPLGLHAGAMVAILTEVTTQRQMERALCEREHQYRALFEENPYPMYIYDQETLAF
                     LAANDAMLRQSGYSRDELLSMTVKEIWPPEDAPAFLESERQNQPGKVMAGVWRHRRED
                     GSTLYVDLFAHSIIYQGRAARVVLAHDVTERIEAEERLKLTTSELSAIFQALPDLYFR
                     LKADGTILDYRAGHEASLYLPPSEFKGRRVQDVLPPPAACHILKGIERTSREHALTTV
                     EYPLPNETGTRIFEARLVPVLDGQVVAIARDITESREAEERLRTSEINYRSIFDAVND
                     AILVHDPATGRILDVNRKMREMYGYTTEETRALTIGDLSANEEPYTLSRALEWVHRAA
                     QGEPQLFEWLAKDRSGRRFWVEVNLKRATIGGEERLLAVVRDITERKRAIQEVEAAHQ
                     RMRRGEDERKRFYREMIRAVTHDKLHLVDAHEIPTVGQLVLSVPLDQPDHYARLRDEL
                     RQIAQKAGMTPETTSDLLMAAGEAATNAIKHATCSESTIYLAPDRIIVRVSDRGSGIQ
                     PEDLPGALLLPGFSTRVSLGMGYTIMLRLADRVWLATSAEGTVVQLEKWIHPEDHQEP
                     ALTSALEKM"
     gene            complement(3989..>4218)
                     /locus_tag="PLY56_08985"
     CDS             complement(3989..>4218)
                     /locus_tag="PLY56_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21656.1"
                     /translation="ACEDPLIRAVVVGGCDGHTYLLVVHFGDRETETAVELGERASAA
                     VDLVSGTEVRLSAGRLALTLGSGEARLARLR"
BASE COUNT          594 a         1420 c         1391 g          813 t
ORIGIN      
        1 ggtggtgttg ctcttcctgc tcgtctcctg gtcgctggta tggcgccgct tctcgccgcg
       61 cctgggccgg acgctggcgc tctctcgctc ggagctggtg ctcatctact tgatggcgac
      121 agtgggcacc gcgctcgcgg gcgagtgttg ggccgtgtat gtcgtgcccg cgctcctcaa
      181 caccgcggca taccaggcgg agacggcgcg cccagagtgg gccgcgtggc tgggggattc
      241 cccctcctgg ttcgccgtgc aggaccccga ggtgatccgc caggcgcatc ggggaaacgc
      301 gtcgctttac cgggtctccg tgttgcgcgc cctcgccgtg ccggtgctgg cctggagcgt
      361 gctgatgctc gcgctggcgc tctttaccca atgcctggcg cagctcgtgc gccgccagtg
      421 gatcgagcgt gagcgcctca gtttcccgct gatccacctc ccgatgacgc tggtggcgtt
      481 cgacgcgccg cgcccctggt ggcgcacgcg ccttttctgg gcgggggtga cggtggccgg
      541 catcatccag ttgatcaacg gcctcaacta cctcgtgccg aacgtcccct acatccccgt
      601 gaagtggatt cagtttcctc cggcctcaga ccgcccctgg tccggcctgg ggcgtttcca
      661 gatcgccttc tatcccttcg tcatcgccat cggcttcatg gtgcccctag aggtcagctt
      721 cagcatgtgg ttcttcttct ggctggggcg cgtgcaggac atggtggtga ccatcttggg
      781 ccatcgcagc gtgggaggcc acgcgccctc gctgccgcct taccatctcc atcaggcggc
      841 gggcgcgttc ctggtccttg gtgccgcatt cctctggcgc agccggcacg agatcgccgg
      901 gatctggcgc aaggccgccg aaccaacggg gcgccgcgcc ggctgggcgc tgatcggaag
      961 cgtgggtgtc atcttggcct gggcctatct cgcgcggatc ccgctccccc tcgcaacgct
     1021 ctttctggtc atttacggcc tcttcgccat cgtcttgggc cggctgattg ccgagagcgg
     1081 cagcgcgatg tcaatgtcgc cgctcggggt gcacgagacg gcggcactcc tcaccaatct
     1141 gggcactcag acgcagcaat cgctggttgc tttcgggtgg tggcatcacc tgggcgagcg
     1201 gctttcagat aacctgctgc cccaccaggt gactggcatg cgcatggccg aggatgtgag
     1261 cgcgggccgc cgtgtgcagc ttttcctggc cgtgggcgcc gtggtcgggg tgctgacagg
     1321 gatctgggcg ttgctccata tctacttcaa gtacgggatc atgtctgccg aagtgcgcga
     1381 ctggccggcg cggtgggcgc cccaatatgc ctacggcctg atccagaact ggctccgcgg
     1441 ccagacggag atcccgcgtg agaagctgat tgccttcgtg gccggcgggg gggtctccgc
     1501 gctgctcctg tggctgcgcc aggcgtacct tggctggccg ctccacccga tcgggtatgc
     1561 cgtggccggg catttcgcca tgcagcagct ctggtgcccc ttctttgtcg cgtggctggt
     1621 gaagctgctg acgatgcgct tcggcgggat ccgcacctac cgcgccttgc ttcccttctt
     1681 cctcggcgcc attctgggtg actacctggt gccgatgggt tgggccgttg tgggccttct
     1741 ttccgggcaa cagatgtacc tgtcgtaccc gcactgacga gcgcgactac atcttctcca
     1801 gggcggaggt gagcgccggc tcctggtggt cctccgggtg gatccacttc tcgagctgca
     1861 ccacagtgcc ctcggcgctg gtggcgagcc agacgcggtc ggccagccgg agcatgatcg
     1921 tgtagcccat cccaagagag acgcgtgtgg agaagccggg gagaagcagg gctcccggga
     1981 ggtcctccgg ctggatgccg ctgccgcggt cggagacgcg cacgatgatg cgatccggag
     2041 ccaagtagat cgtgctctcg ctgcaggtgg cgtgtttgat cgcgttcgtc gcggcctcgc
     2101 cggcagccat caggagatcg ctggtggttt cgggagtcat cccggccttc tgggcaatct
     2161 ggcgcagctc gtcgcgtagg cgcgcgtagt ggtccggctg gtcgagtggc acgctgagca
     2221 ccagctggcc cacggtaggt atctcgtgcg cgtcgaccag gtggagctta tcgtgggtca
     2281 ccgcgcgaat catctcgcga tagaagcgct tgcgctcatc ctcgccccgg cgcatgcgtt
     2341 ggtgggccgc ctccacctcc tgaatcgcgc gcttgcgctc ggtgatgtcg cgcaccaccg
     2401 cgagcaggcg ctcctcgcct ccgatggtgg cacgcttcag attcacctcc acccagaagc
     2461 gccggcccga tcgatccttc gccagccact cgaacagctg gggctcgcct tgcgccgccc
     2521 gatgcaccca ctccaaggcc cgcgagagtg tatagggctc ctcgttggcg ctcaggtcgc
     2581 cgatcgtcag cgcccgcgtt tcctcggtcg tgtagccata catctcgcgc atcttgcggt
     2641 tgacgtccag gatccgcccg gtggcgggat cgtgaaccag gatggcgtca ttcacggcgt
     2701 cgaagatgga gcggtagttg atctccgagg tgcgcagccg ctcctcggcc tccctactct
     2761 cggtgatatc gcgggcgatg gcgaccacct ggccatcgag gacgggcacc agtcgggcct
     2821 caaagattct ggtcccggtt tcgttcggca ggggatactc cactgtcgtg agagcgtgct
     2881 cgcgcgaggt gcgctcgatt cccttcagga tgtggcacgc ggccggcggc gggaggacat
     2941 cctgcacccg tctgcccttg aactcggaag gaggcaggta cagactggct tcgtgtcccg
     3001 cacggtaatc gagaatcgtc ccatcggcct tcagccggaa gtagagatcc ggcagcgcct
     3061 ggaagatggc gctcagctcc gaggtagtca gcttcagccg ctcttcggcc tcgatccgtt
     3121 cggtgacatc gtgcgcgagg acgacgcgcg cggcccttcc ttgatagatg atcgagtgtg
     3181 cgaagagatc gacgtagagg gtcgagccgt cctcgcgccg gtgccgccag acgcccgcca
     3241 tcaccttgcc gggctggttc tggcgctcac tctccaagaa cgcgggcgca tcctccggcg
     3301 gccagatctc cttcaccgtc atcgagagga gctcgtcgcg cgagtagccg gactggcgga
     3361 gcatcgcgtc gttcgcggcc aggaacgcga gcgtttcctg gtcgtagatg tacatcggat
     3421 agggattctc ttcgaagagg gcgcggtact ggtgctccct ctcgcaaagc gcgcgctcca
     3481 tctggcgctg cgtcgtgacc tccgtcagga tcgccaccat cgcaccagcg tgaaggccca
     3541 gcgggtggca ctggaccagg agatcgcggc tctcaccccc cagttgggcg tgcacttcgc
     3601 gtggaggcag gttgccgcca gcggctgcgg cctcccggag cggtggcagc agatcggaga
     3661 gcacgccgcg ctgcacttcc tcggagaggg tcacgcccac gatctcggcg gggtttcccg
     3721 cagccgcgag tgaagccaag gcctggttcg cggcaaggat gcggagcgag ggaatctcca
     3781 gagcgcagac cgccagcggg acctgcccca ggattcccac aaggtcttct acattgactg
     3841 ccgctgccat tgcgaaccgc atgccactcc ccattcccct ctgcgctggc agcgcatagg
     3901 ctgcgggcca ttccgcccag ccggcgctcg aagtctgagc attctacgcg ccgttctgtt
     3961 gcctgccgca gagtgcctgc cgcacatcct atctcagccg cgccagccgc gcctcgccgg
     4021 atccgagcgt aagcgcaagc cggccagcgc tcaaccgcac ctcggtgccg gagacgagat
     4081 ccacggccgc ggaagccctc tcgcccagct ccaccgcggt ctccgtctcc ctatctccga
     4141 aatggacgac gagcagatag gtgtgcccgt cgcatccccc gacgaccacc gcgcggatga
     4201 gcggatcctc gcaggccg
//
LOCUS       DAOSCV010000325         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13250_length_4215_cov_2.968750, whole genome shotgun sequence.
ACCESSION   DAOSCV010000325 DAOSCV010000000
VERSION     DAOSCV010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13250_length_4215_cov_2.968750"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(400..486)
                     /locus_tag="PLY56_08990"
     tRNA            complement(400..486)
                     /locus_tag="PLY56_08990"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(450..452),aa:Ser,seq:tga)
     gene            complement(763..2190)
                     /locus_tag="PLY56_08995"
     CDS             complement(763..2190)
                     /locus_tag="PLY56_08995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ21657.1"
                     /translation="MDELSIRHILVVEDDPDHAMLAMRTLHRYFRDVTIQLATSAEEG
                     EALLADGEWDAVVVDYGLPCANGLHLLEAARDRRPHLPIVMLTGRGDEQTAVAAMKGG
                     AADYVTKTGDYLALLPAAVDAAVRRSRVAADRERLARELEQRNRHLAAVSTVAVAVSR
                     SLHLDEMLQEVVTHVTSISGLPAALFLPDGQSTRLRLAAHANLAPAIVSCLENYGLPV
                     PPAGATSAGATVRVDALAGLSVEPRFFSLAADGEPQGLLAVFPEKGRTLDAPEAEMLG
                     TLAHVVGMGVRNSLLFEETRSLSLRDELTGLGNRRLFNIKLQSDFNTARRYGTRLALL
                     MLDIDHFKAINDCYGHPSGDWVLRRLAAVLLERSRSSDLTFRYGGEEFAMLLPHTSDE
                     AAVRVAERVRRIVAAEEFAVRGAVVQITVSIGIGCYREAMADETELINAADRALYGAK
                     QSGRNCVCLCDGNRLRLAETLVSSR"
     gene            complement(2309..>2569)
                     /locus_tag="PLY56_09000"
     CDS             complement(2309..>2569)
                     /locus_tag="PLY56_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21658.1"
                     /translation="PDLVLLDLNLPKVDGLSVLEEIKGSEGLRTIPVVVLTTSQRQDE
                     VKRAYGAGANSYVTKPVQFADFSEKIRMVGAYWLSTSSLPGN"
     assembly_gap    2570..2579
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2580..2719)
                     /locus_tag="PLY56_09005"
     CDS             complement(<2580..2719)
                     /locus_tag="PLY56_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21659.1"
                     /translation="MVEDDPDHAILTIRSLRKAGFEQQVVWVKDGREALEHLPDLLLV
                     DA"
     gene            complement(2950..>4215)
                     /locus_tag="PLY56_09010"
     CDS             complement(2950..>4215)
                     /locus_tag="PLY56_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ21660.1"
                     /translation="ALENMRLTVEAQDRARIERLVNRISQRIQSTLDVDTVMGTAVRE
                     LCVAAGASRCLIMRHQEGAPATVTHEYCQPGIEPVGVGREPAAGVCQRALVTDSTVIC
                     DDVLHDPDLSPEDREELLSLGTRAALATALRHRDQIFGAVTLHECGRPRQWRDADVAL
                     VETVAGQIAVGVENALLYEETRRKSEEISAKNRELEAFVYTASHDLRAPLVTIQGFAS
                     ILLSDHGDALDEDAHFYLERIRTNTGRMERLIDDLLELSRAGRATGQFMDVPVGEVVA
                     EALDALHARLREREVELVVADSWPTIACDRERLLQVFTNLVGNAVKFLGPDNKHPRIE
                     LGWCEREESAEFFVRDNGIGIEPDYHERIFAVFQRLNDLEGVEGTGVGLAIVKRIIDL
                     HGGRVWVESSRGEGATFRFTLPWRREASS"
BASE COUNT          796 a         1451 c         1251 g          707 t
ORIGIN      
        1 ctccgacaca tattggcatg acaaacaaac aagacatgca tggggagagc cattgagctc
       61 ccttcgcaac acctaggagg ccggaagccc gcgggcttcc cacgcaggaa ggaaacgcct
      121 ctcacccgca tgggcgcttc ttcccccagg tcgaacgggg ctctggccac gccccgtcac
      181 cctcgctctc ccacacgcag gcacgccagc aggagacgcc caccacacaa atccctctct
      241 cataccctta tctcacgcgg gatgatgcca gccgcagatc caaatagcaa agagagggga
      301 tgtggggact tctctcacgt tcacgcgccg acctcacgag ttccacgatg gaaccgcgcg
      361 cgtccgcgcc acgggagata accgagatgt atcttgattt ggcggaaggg gagggattcg
      421 aacccccggt cctcatcgga ctactgtttt caagacagtc gcgatcgacc actccgccac
      481 ccttccatac catatagatc ctacacgacg caagcggtat ctgtcaagag ttttcagtca
      541 cgctcgttca ccagcagcgg cgcgtgcagg cgggcgatcc cagtgggaat gccgtgggcg
      601 ccggccacaa tggcgaggcg atcacgctgg gccaagggag cagcatgcgc gtagttcccg
      661 tctccggaac ggccgtgcgc ctgggatagg ccgcggcacg ggggtcaggg ccgagcgcag
      721 catcgctagg cgacaggagc tgatgggaaa gagcggtcga ggctatcgcg aggagaccag
      781 cgtctccgcc aggcgtagcc ggttgccatc gcataagcag acgcaattcc gtccagactg
      841 cttggcgcca tagagcgccc ggtcggccgc gttgatcagc tccgtctcat ccgccatcgc
      901 ctcgcggtag cagccaatgc cgatgctgac cgtgatctgg acgacggcgc cgcgcacggc
      961 aaactcttcc gcggccacga tccggcggac gcgctctgcc acgcgcaccg ctgcttcatc
     1021 actcgtgtgc ggcaggagca ttgcgaactc ttccccacca tagcggaagg tgagatcaga
     1081 gctccggctg cgctccagga gcactgcggc caggcgtctc aagacccagt caccggatgg
     1141 atgcccatag cagtcgttga tcgccttgaa gtggtcgata tcaagcatga gcagcgccag
     1201 gcgcgtgcca tagcgcctgg cggtgttgaa atcactctgg agcttgatgt tgaagaggcg
     1261 ccggttgccc aatccggtca gctcatcgcg cagcgacagc gaccgggtct cctcgaagag
     1321 gaggctgttg cgaacgccca tgcccaccac gtgggccaac gtcccgagca tctccgcttc
     1381 gggcgcgtcc agcgtccttc ccttctctgg aaagaccgcc aggagtcctt gtggctcccc
     1441 atccgccgcg agcgagaaga accgtggttc cacagacaac ccggccagcg catccacccg
     1501 aacggtagcc cctgcgctcg tcgcacctgc ggggggcaca ggcaggccgt agttttccag
     1561 gcaacttacg atcgctggcg ccagattcgc atgggccgcc aggcgcaggc gcgtgctctg
     1621 gccatcaggc aggaagagcg cagccggcag gccggaaatc gacgtcacgt gcgtgacgac
     1681 ctcctggagc atctcatcca gatgcagcga gcgagagact gccacagcca ccgtgctcac
     1741 ggcggccaga tggcggttgc gctgctccaa ctccctggct agccgctccc gatcggcagc
     1801 gacacggctg cgccgcacgg ccgcatccac cgccgccggc agcaacgcca gatagtcgcc
     1861 cgtcttggtc acgtagtcgg ccgcgccgcc cttcatggcg gcgaccgccg tctgctcatc
     1921 gccacggccg gtcaacatca caatcggaag atgtggtctg cggtcgcgcg ccgcttccag
     1981 gagatgcaag ccgttggcgc agggcaatcc gtagtcgacg accaccgcgt cccactcgcc
     2041 atcggcaagc agcgcctctc cttcctcggc agaagtcgcc agctggatcg tgacgtcacg
     2101 gaaatagcga tggagcgtgc gcattgccag catggcgtgg tcggggtcat cctcgaccac
     2161 cagaatatgt cggatgctca gttcatccat cagcgacgca ggtgtccatc ccgcctgatg
     2221 ccaggccacg atcgcggatg ctttcggcag cgcgcgtctc gccaggtatc ctggggatct
     2281 agtgagagcg cggccacgcc tcgcccgctc agtttccggg caggctgctc gtggacaacc
     2341 aataggcgcc caccatccgg atcttttccg agaaatcggc gaactgcacc ggcttcgtga
     2401 cgtaggagtt ggcgccagcg ccgtaggcgc gcttcacctc atcctggcgt tgagaggttg
     2461 tcagcaccac caccgggatg gtgcgcaggc cttccgagcc cttgatctcc tctagaacgc
     2521 tcaagccatc taccttcggc aagttcagat cgagcaacac caggtccggn nnnnnnnnnt
     2581 cggcgtcaac gagaagcagg tccggaagat gctcgagggc ctcacgccca tccttcaccc
     2641 atacgacctg ctgctcgaag ccggccttac gcaaagatcg aatcgtcagg atcgcatggt
     2701 cgggatcatc ctcgaccaac agaatgcatg ctggagagga gttcaaactg cttcttcctc
     2761 cctgaaagaa cgcctaccac tcactcatgg ggtcacaccg ggcggcgcac ccactgcggc
     2821 gacggcgccc ggaacgagag cccgcgagct cccgctgccc ctcccacctt atccaacggc
     2881 cacttcacga ctttcctata gccgcgctcc gcccgcgtcc cactgtctcg cgagttcgcc
     2941 ccagctccct cacgatgagg cctcgcgcct ccatgggagc gtgaagcgga aagtggcccc
     3001 ttccccccga gaagactcga cccagacccg gcccccgtgg agatcgatga tccgtttcac
     3061 gattgccagg ccaaccccgg tgccttccac cccctccagg tcattcagcc gctggaagac
     3121 agcgaagatc cgttcgtggt aatccggctc gatgccaatc ccgttatcgc gcacgaagaa
     3181 ctcggcgctc tcctcgcgct cacaccaccc gagctcgatg cgcgggtgct tgttgtctgg
     3241 cccgaggaac ttcaccgcgt tgcccaccag attcgtgaag acctgaagca gacgctcgcg
     3301 gtcgcaggca atcgtgggcc agctgtcggc gacgaccagc tcgacctcgc gctcgcgcag
     3361 gcgcgcgtgg agagcgtcga gcgcttcagc gaccacctcg cccacgggaa catccatgaa
     3421 ttgtccggta gcgcggcccg ctcgcgacag ctcgagaagg tcatcaatca agcgctccat
     3481 tcgccccgtg ttggtgcgaa tgcgctcgag atagaaatgc gcgtcctcat ccaacgcatc
     3541 cccatgatcg gagagcaaga tcgaggcaaa cccctggatc gtgacgaggg gcgcgcgcag
     3601 atcatgcgac gccgtgtaaa cgaaggcctc cagctcgcgg ttcttggcgg agatctcctc
     3661 gctcttgcgc cgcgtctcct cgtacagaag agcgttttcg acgccgaccg cgatctgtcc
     3721 tgccacggtc tcgaccaggg ccacgtccgc gtcgcgccat tggcggggcc ggccacactc
     3781 gtggagggtc actgccccaa agatctggtc gcgatggcga agcgcggtag ccaaggccgc
     3841 gcgcgttccg agggagagta gttcctcgcg atcctcgggc gagagatcgg gatcgtgcag
     3901 cacgtcgtcg cagatcaccg tgctatccgt caccagcgca cgctggcaga cgcctgcggc
     3961 tggctcgcgc cccaccccca ctggctcgat acccggctgg caatactcgt gagtcacggt
     4021 ggcaggcgca ccttcttgat ggcgcatgat cagacagcgg ctcgcaccgg cggccacgca
     4081 gagctcgcgc accgcggtcc ccatcacggt atcgacatcc agcgtgctct gaatgcgctg
     4141 gctgatccgg ttcaccaggc gttcgatgcg cgcgcggtcc tgcgcttcta ccgtgagacg
     4201 catattctcg agggc
//
LOCUS       DAOSCV010000326         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13306_length_4203_cov_3.610656, whole genome shotgun sequence.
ACCESSION   DAOSCV010000326 DAOSCV010000000
VERSION     DAOSCV010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13306_length_4203_cov_3.610656"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLY56_09015"
     CDS             <1..1130
                     /locus_tag="PLY56_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ21661.1"
                     /translation="AAIIRKNLHRSALYGGRIEGRGPRYCPSIEDKIVKFPDRERHQI
                     FLEQEGWHTDEIYVQGASTSLPEEVQLAFLRSIPGLEEVRVLRYGYAVEYDALPPEQL
                     KPTLETKRVAGLFSAGQVNGTSGYEEAAAQGLIAGINAALQIQGRPAFTLGRADAYIG
                     VMIDDLITKGVNEPYRLLTSRAEHRLLLRQDNADLRLTAKGRAVGLVSDERWRRFCEK
                     QRQIDLALDRLNRVMLRPTASVQESLRALGTAELPKPVELAQILRRPGIRYADLRALD
                     PEMPELPPDVVEQVEIEVKYAGYIERQRAQVEHHRRLEKRLIPEGFPYEQVRALSHEG
                     REKLMRVRPASVGQAARIPGLTPADISVLLVALERASSPRQ"
     gene            complement(1124..1591)
                     /locus_tag="PLY56_09020"
     CDS             complement(1124..1591)
                     /locus_tag="PLY56_09020"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOJ21662.1"
                     /translation="MDERFMRMAIEKAREGVAQGGSPFGACIVRDGEVVACAHNRVWQ
                     ETDITAHAEIVAIREACRKLGTIDLSGCEIYSTCEPCPMCFAACHWARLDRVIFGARI
                     ADAAACGFNELPISNREMKEKGGSPVEITGDFLRDECVELFRSWRGKEEGRTY"
     gene            complement(1654..2160)
                     /locus_tag="PLY56_09025"
     CDS             complement(1654..2160)
                     /locus_tag="PLY56_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21663.1"
                     /translation="MKRIVDTNVIVVTNGRSEQVSPACVMECARQLKAITGGACTLVL
                     DDAFHILREYRSNCSESGQPGLGDAFLKWVLTNWRDPRRCECVHITQLGDDNTAFSEF
                     PDDDSLAGFDPSDRKFVAVALAHPERPPILQAADAKWWTYRDALRAFGVEVLFLCGPD
                     AAAFTGGA"
     gene            complement(2157..3527)
                     /locus_tag="PLY56_09030"
     CDS             complement(2157..3527)
                     /locus_tag="PLY56_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HOJ21664.1"
                     /translation="MITRLRLENFKSWEDTGDLRLGRLTGLFGTNSSGKTSLLQALLM
                     MRQTVESRDRSRVVHTGDDRSLVDLGTFHDLLYNHDAERPLRLSFSWTLPERLSIANP
                     ETPEGVLYRISALSFTTEIRERQERPVVESFAYQFDGHCFGMRRREENGHEKDAYDLN
                     TGDFPAKRVQGRPWPLPSPVKCYGFPDEATGYYQNTGFLPDLVLALEGLFSRLSYLGP
                     LREYPHRTYIWGGDRPLDVGQRGEQAVAALLAARAADLKSGRGEGKGRRYKPIEARIA
                     EWLRKAGLIHSFELRPVAEGRKDFEVKVKTARDSAEVLITDVGFGVSQLLPVLVLCHY
                     VPEGSTILLEQPEIHLHPSVQAALADLFIDVVINRNVQIIVESHSEHLLRRLQRRIAE
                     EAIAPDDTALYFCRREGGASVADKLEVDLYGNIVNWPAGFFGDEMGDLFAKTEAEMRR
                     RAGIPR"
     gene            3706..>4203
                     /locus_tag="PLY56_09035"
     CDS             3706..>4203
                     /locus_tag="PLY56_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ21665.1"
                     /translation="MTSIAEELQQRLQGEVHFDHFTRALYSTDASIYREEPLGVVMPR
                     DEEEVAATVALAARAQVPLLPRGAGTSLAGQTVGRALVLDLSRHMDRILEINPEERWA
                     RVQPGVVLDRLNAALRPHGLMVGPDVATSNRACFGGMIGNNSSGARSRVYGITSDGVL
                     SMRAVL"
BASE COUNT          740 a         1410 c         1344 g          709 t
ORIGIN      
        1 ccgccgcgat catccggaag aacctgcacc gctcggcgct ctacgggggg cgaatcgagg
       61 ggaggggccc gcgctactgc ccgagcatcg aggataagat cgtgaagttc ccggaccggg
      121 agcggcacca gatcttcctc gagcaggagg gatggcacac cgacgagatc tatgtccagg
      181 gcgcgtccac cagccttccg gaagaggtgc agctcgcctt cctccgctca attcccgggc
      241 tggaggaggt gcgagtcctt cggtatggct acgcggtgga gtatgacgcg ctaccaccgg
      301 agcagctcaa gcccactctg gagacgaagc gcgtagcagg cctcttctcg gccgggcagg
      361 tgaacgggac gtccggctac gaggaggccg cggcgcaggg cctgatcgcg gggatcaatg
      421 cagcgctcca gatacagggc cggcccgcgt tcacgctcgg acgtgccgac gcctacatcg
      481 gcgtgatgat cgacgacctg atcaccaagg gcgtgaacga gccgtaccgg ctgctcacct
      541 cgcgtgcgga gcatcgcctc ctgctgcgcc aggacaacgc cgatctccgc ctcaccgcca
      601 aggggcgcgc ggtcggcctg gtgagtgatg agcgctggcg gcggttttgc gagaagcagc
      661 gccagatcga cctcgcgctc gaccggctga accgcgtcat gctccggccc accgcgagcg
      721 ttcaggagag cctgcgcgcc ctcggcaccg cggagctacc gaagcccgta gagcttgcac
      781 aaatcctgcg ccggcccggg atccgctatg ccgacctgcg cgcgctcgat ccagagatgc
      841 cggagttgcc gccggacgtg gtggagcagg tcgagatcga ggtcaagtac gcgggctata
      901 tcgagcggca acgcgcgcag gtggagcacc atcgtcgcct ggagaagcgc ctcattccag
      961 agggctttcc ctatgagcag gtgcgcgcgc tttcgcacga gggccgcgag aagctgatgc
     1021 gcgtgcgccc ggcgagcgta ggtcaagccg cgcgcatccc cgggctcacc ccggctgaca
     1081 tctcggtcct gcttgtcgcg ctcgagcgtg cgtcgagccc gcgtcagtag gtacggccct
     1141 cttctttccc gcgccacgac cggaacagct ccacgcactc gtcgcgtagg aagtcgccgg
     1201 tgatctcgac cgggctgccc cccttctcct tcatctcgcg gttggagatc gggagctcgt
     1261 tgaagccgca cgcggccgca tcggcgatcc gcgcgccaaa gatcacgcgg tccagtcttg
     1321 cccaatggca cgcggcgaag cacatcgggc acggctcgca cgtcgagtag atctcgcacc
     1381 cggagaggtc gattgtcccc agcttccggc aggcttcgcg tatcgcgacg atctcggcgt
     1441 gcgccgtgat atcggtctcc tgccacaccc ggttgtgcgc gcaggcgacc acctcgccat
     1501 cgcgaacgat gcacgccccg aaaggcgacc cgccctgggc cacgccctct cgtgccttct
     1561 caatcgccat ccgcatgaaa cgctcgtcca tcttcctccc tccacgtgac atggcagcgc
     1621 ccgccctaaa aacacccttt ctctggctgt tgctcaggcg ccgccggtga aggccgcggc
     1681 atcggggccg cagaggaaaa ggacctcaac gccgaacgcg cgcagggcat cgcggtaggt
     1741 ccaccacttg gcatctgccg cctggaggat cggcgggcgc tccgggtgag ccagtgccac
     1801 ggcgacgaac ttgcggtcag atgggtcgaa gccggcaagc gagtcatcgt cagggaattc
     1861 actgaaggcg gtattgtcat cgcccaactg agtgatgtga acgcactcac agcgacgcgg
     1921 atcccgccag ttcgtcagga cccacttcag gaaggcatcg ccgagtcccg gctgaccaga
     1981 ctcgctgcag ttcgagcggt attcccggag gatgtggaag gcgtcgtcca ggaccaacgt
     2041 gcaggcgcct ccggtgatgg ccttgagctg tctggcgcac tccatcacgc aagcggggga
     2101 tacctgctcg gagcgcccgt tggtgacgac gatgacgttg gtgtcgacga tccgcttcat
     2161 cgcgggatac ccgcccgccg gcgcatctcc gcctcggtct tggcgaagag atcccccatc
     2221 tcatcgccga agaagccggc cggccagttg acgatgttgc cgtagagatc cacttccaac
     2281 ttgtcggcta cggatgctcc gccctcgcgc cgacagaagt agagcgcggt atcgtcgggg
     2341 gcaatcgctt cctcggcgat gcgccgctgc aggcgccgca gaagatgctc gctgtggctc
     2401 tccacgatga tctgcacgtt gcgattgatc accacgtcga tgaagagatc ggcgagcgcc
     2461 gcctgcaccg agggatgcag gtgaatctcc ggctgctcca ggaggatggt cgatccctcc
     2521 ggcacgtagt ggcagaggac gagcaccggt agtaattgag agacgccgaa gcccacgtcc
     2581 gtgatcagca cctcagcgct gtctctcgca gtcttgacct tgacctcgaa gtccttacgg
     2641 ccctccgcga caggccgaag ctcgaaggag tgtatcaggc ccgctttgcg gagccactcg
     2701 gcaatcctgg cctcgatagg cttgtagcgc ctgcccttcc cttcccctcg cccgcttttc
     2761 aggtcggccg cgcgtgccgc caagagcgcg gccacggcct gctcgccacg ctgtccaacg
     2821 tcaagcgggc gatcaccgcc ccagatgtag gtgcgatggg gatactcccg aagtgggccc
     2881 aggtagctca gacggctgaa aagcccctca agcgccagaa ccaggtccgg cagaaaaccc
     2941 gtgttctggt agtagccggt ggcctcatct ggaaacccgt agcacttcac cggagacggg
     3001 aggggccagg gccgaccctg cacacgtttg gccgggaaat cgccagtgtt caggtcgtaa
     3061 gcgtccttct catgcccgtt ctcttcccga cggcgcatgc caaagcagtg gccgtcaaac
     3121 tggtacgcaa agctctcaac aaccgggcgt tcctgacgct cgcggatttc cgtcgtgaat
     3181 gaaagcgcac tgatccggta aagcacgccc tcaggcgtct cggggttggc aatcgagagc
     3241 cgctcgggaa gcgtccagga gaacgagagc ctgagagggc gttctgcatc gtggttgtac
     3301 agcaggtcgt gaaatgttcc gaggtctacg agggacctgt catcacctgt gtgaaccacg
     3361 cgagaccggt ccctggattc gacagtctgc cgcatcatga ggagcgcctg aagcaggctc
     3421 gtcttcccgg agctgttggt accgaagagg cccgtgaggc ggcccagccg caagtctccg
     3481 gtatcctccc atgacttgaa attctccaag cgcaagcgcg tgatcatggc aaccgtctcc
     3541 cctgcctctc ttcgccgcgc gccagggctt ccccttgcga acccgtgcag gccggaggcc
     3601 tcgacaagca cacctgccca cgctcgacac attctacggt cccgacctgc tccttgccgg
     3661 gcgttcctgc ttgcggtata atgaggcagg aaaagagcgc gcgccatgac atccatagcc
     3721 gaggaactcc agcagcgcct gcaaggggaa gtccatttcg accactttac ccgggcgctc
     3781 tacagcaccg atgccagcat ctaccgcgaa gagccccttg gggtggtgat gccccgcgac
     3841 gaggaggagg tggccgcgac cgtcgccctc gccgcgcgcg cgcaggtccc gctcttgccc
     3901 cgtggcgccg gcaccagcct cgccgggcag accgtcgggc gtgccctggt gctcgacctc
     3961 tcccggcaca tggatcgcat cctggagatc aaccccgagg agcggtgggc gcgtgtgcag
     4021 ccaggcgtgg tgctcgaccg cctgaacgcc gcgctccgcc cacatgggct gatggtcggc
     4081 ccggatgtgg cgacgagcaa ccgcgcctgc tttggcggga tgattgggaa caactcgtcg
     4141 ggcgcgcgct cgcgggtcta cgggattacc tcggacgggg tgctttcgat gcgtgccgtg
     4201 ctt
//
LOCUS       DAOSCV010000327         4199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13328_length_4199_cov_3.086873, whole genome shotgun sequence.
ACCESSION   DAOSCV010000327 DAOSCV010000000
VERSION     DAOSCV010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13328_length_4199_cov_3.086873"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PLY56_09040"
     CDS             complement(<1..929)
                     /locus_tag="PLY56_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOJ21666.1"
                     /translation="MRSIFKACDVRGVYGRELTEEKVCRIGAAVATLLRRRGRPACIL
                     VGGDVRVSTPSLKAALVAGLTECGAECLDLGTVPTPVFYFARQHLGVPAGVMVTASHN
                     PASHNGLKLVLGELPVTPEDLGEIRGMAEAGDFEKAPGSAAVVSVADAYARHLLEATA
                     PWHAPSKEAPRIVVDPGGGCWSRWAASLLRDAGYPALSIHDTPDGRFPFRDPNPSVPE
                     HLLALREAVVAEGAGLGVAFDGDGDRVAFVDERGRALPADETAILLIRHLVPGEPGAR
                     VAHDIKCSQAVADEVRRCGGVPLMERSGHTFLKT"
     gene            complement(983..2461)
                     /locus_tag="PLY56_09045"
     CDS             complement(983..2461)
                     /locus_tag="PLY56_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ21667.1"
                     /translation="MRQTAVNGKETGRVVIGLDVGTQGARALAVSADGEVVAAASRRL
                     EGAGPALPEGYSEQDPEAWWAAARACLREVAGRAGAERVACLAVTSTSGTVVSVDAAG
                     RALRPAIMYNDGRAVDEAAEVQAAGRAQAERLGYQFQPSFGLPRLLWIRRHEPGVFER
                     AACFLHAADFLVARLTGEVGRSDSSNALKTGYDLENLCWGDWIERDLGIPLAKLPRVV
                     QPGEAIGALSRAAAEETGLRVGTPVAAGVSDGTAAFLASGAISPGEWNSTLGTTLVVR
                     GVSANLVRDPRGRLYCHRHPEGYWLPGGASNTGGEWLEHRFPDADWLALDQSALSLSP
                     TEQVVYPLVRRGERLPFIDPRAEGFIEAPAGVAPERLYAAHLEGTACVERWIYEVIES
                     LGVPVGPRVFATGGGARSREWLQIRADLLGRVLVRPRVVEAAFGAALLAAARTLHPSL
                     SDAVRSMVRADCKVTPRPGLRAAYDELYARFRETCARRGYVG"
     gene            complement(2531..3277)
                     /gene="kdsB"
                     /locus_tag="PLY56_09050"
     CDS             complement(2531..3277)
                     /gene="kdsB"
                     /locus_tag="PLY56_09050"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOJ21668.1"
                     /translation="MNAVVIIPARYASTRLPGKPLAEIAGRPLIQHVYERASRARGIA
                     EVVVATDDERIRDAVLAFGGKVAMTSSEHRTGTDRVAEAAEAYDAEIVVNVQGDEPLI
                     PPEVVGKVAGALEADETVAMASAMVRVRTPEERDAPSVVKVVVDQQGNALYFSRYPIP
                     YVRDAGVTHMPYKHLGIYAYRREFLRLFTRLSQTPLERLESLEQLRALEHGYRIRMVE
                     VEYDPVSVDTPEDLARVRALMEQGSASPAR"
     gene            complement(3274..3762)
                     /gene="rfaE2"
                     /locus_tag="PLY56_09055"
     CDS             complement(3274..3762)
                     /gene="rfaE2"
                     /locus_tag="PLY56_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOJ21669.1"
                     /translation="MNVARKKILTWDELAREVSRRKREGQRIVFTNGCFDLLHVGHAR
                     YLQQARALGDCLIVGLNSDESVRALKGPNRPLIPAEERAEMLAALECVDAVAIFPQRT
                     PDALIRLVAPHVHVKGGDYRKEDLPEAALVEALGGEVCIVPLVEGRSTSEIVLRILER
                     YR"
     gene            complement(3759..>4199)
                     /locus_tag="PLY56_09060"
     CDS             complement(3759..>4199)
                     /locus_tag="PLY56_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ21670.1"
                     /translation="NPKPPNLLRLRGATAVTLNHLEAEAAAGRRLQDEGALREAGQVM
                     RSELELDALLVTRGSRGMMLFSNDGHCRAIPAVPVEVYDVVGAGDTAFATLSLALVAG
                     AGFEEAATLANLAGAAVVRKVGTAVATPDELLAMIEECASGVGE"
BASE COUNT          672 a         1606 c         1355 g          566 t
ORIGIN      
        1 gtcttcagga aggtgtgccc cgagcgctcc atcagcggca cgcccccgca ccggcggact
       61 tcgtcggcga ccgcctgcga gcacttgatg tcgtgcgcca cccgggcgcc cggctcgccc
      121 ggcaccaggt ggcggatcag gaggatcgcc gtctcatcgg ccggcagggc gcgcccacgc
      181 tcgtcgacaa acgccacccg gtcgccatcg ccatcaaacg ccacgccaag tcccgccccc
      241 tcggccacga ccgcctcgcg caacgccagg agatgctccg gcacggaggg gttgggatcg
      301 cggaagggaa aacgcccgtc gggcgtgtcg tggattgaga gagccggata gccggcgtcg
      361 cgcaggagac tggccgccca cctggaccag cagccgcccc ccgggtcgac cacgatccgc
      421 ggagcctcct tggatggggc gtgccacggc gccgttgcct ccaggaggtg ccgggcgtag
      481 gcatcggcga cggagaccac ggctgccgag ccgggcgcct tctcgaagtc gccggcctcc
      541 gccatgccgc ggatctcgcc cagatcctcc ggcgtcaccg gcagctcgcc cagcaccagc
      601 ttcaggccgt tgtgcgacgc ggggttgtgt gacgcggtca ccatcacccc ggcaggcacg
      661 ccgagatgct gccgggcaaa gtagaagacg ggagtcggca ccgtacccag gtcgaggcac
      721 tccgcgccgc actcggtgag accagccacg agcgccgcct tgagcgaagg cgtgctcacg
      781 cgcacgtcac cgcccaccag aatgcacgcc gggcgcccgc gccggcgcag gagcgtcgcc
      841 acggcggcgc caatccggca caccttctcc tcggtcagct cccggccata gacgccgcgc
      901 acgtcacacg ccttgaagat gctccgcatt caggccacac cgcctatccc gggaaggccg
      961 cgccgccgcg agcccccctt tctcatccaa catacccccg gcgcgcgcac gtctcccgga
     1021 agcgcgcgta gagctcgtca taagcggcgc gcaggccggg ccggggcgtc accttgcaat
     1081 cggcgcggac catgcttcgc accgcgtccg agagcgaggg atgcaacgtg cgcgccgccg
     1141 ccaggagcgc cgcgccaaac gccgcctcaa cgacgcgcgg ccggacgagc acgcgcccga
     1201 gcaggtccgc gcggatctgt agccactcgc ggctgcgcgc acctccgccc gtcgcgaaga
     1261 cgcgaggccc caccggaaca ccgagcgatt cgatcacctc gtagatccac cgctccacgc
     1321 acgcggttcc ctccagatgc gccgcgtaga gccgctccgg cgcgacgcca gcaggagcct
     1381 cgatgaatcc ctcggcgcgc gggtcgatga aggggaggcg ctcgccgcgc cggaccaggg
     1441 ggtaaaccac ctgttctgtc ggcgaaaggc tcagcgcact ttggtcgagc gccagccaat
     1501 cggcgtctgg aaagcgatgc tccagccact cgccgccggt gttgctggca ccgccgggaa
     1561 gccagtagcc ctccggatgg cggtggcagt agaggcgccc tcgcgggtcg cggaccaggt
     1621 tcgcgctcac gcctcggacc acgagcgtcg tgccgagcgt ggagttccac tcgcccggcg
     1681 agatcgcgcc cgacgcgaga aacgcggcgg tgccatccga gacgccagcg gccacagggg
     1741 tgcctacacg caggcccgtc tcctctgccg cggctcggga gagcgccccg atcgcttcgc
     1801 ccggctggac cacgcggggc agcttagcca gggggatacc caggtcgcgc tcaatccaat
     1861 cgccccagca gaggttctcg aggtcgtagc cggtcttcag cgcgttcgaa gagtccgagc
     1921 gccccacttc gcccgtcagc cgggccacca ggaaatcggc cgcgtggaga aagcaggcag
     1981 cgcgctcaaa cacccccggc tcgtgccgtc ggatccagag aagccgcggg agcccgaacg
     2041 agggctggaa ctgataaccc aaccgctccg cttgggcgcg cccggcggcc tgaacctcgg
     2101 cggcctcatc caccgcgcgc ccgtcgttat acatgatcgc cggacgcaac gcccggcccg
     2161 cagcatccac cgacaccacc gtgcccgaag tggacgtgac tgccaggcag gccacgcgct
     2221 ccgcgcccgc cctaccggcc acctcgcgca ggcacgcgcg cgccgccgcc caccacgcct
     2281 ccgggtcttg ctccgaatag ccctccggaa gcgccggtcc cgcgccctcc aggcgcctgg
     2341 acgccgccgc cacgacctcg ccatcggcgc tcaccgcgag cgcgcgcgcc ccctgcgtcc
     2401 ccacatcgag cccgatgaca acgcggccgg tctctttccc gttgacggcg gtctgtcgca
     2461 tggcatccct ccggtcagat cgcgaagccg cgcccgcgcg gggcctcagg gcgcgggaga
     2521 cgtctcgggg tcagcgcgcc gggctggcgc tcccctgctc catcagagcg cgaacccgcg
     2581 cgagatcctc tggcgtgtcc acgctcaccg gatcatattc gacttcgacc atccggatcc
     2641 ggtagccgtg ctccagagcg cggagctgct cgagcgattc gaggcgctcc aggggcgtct
     2701 gcgacaggcg cgtgaagagg cgcagaaact cccggcgata ggcgtagatt cccaggtgct
     2761 tgtagggcat gtgcgtcacg cccgcgtcgc gcacgtacgg gatcgggtag cgagagaagt
     2821 agagggcgtt gccctgctgg tcgaccacga ccttcacgac cgagggagcg tcgcgctctt
     2881 ccggcgtgcg aacgcggacc atcgccgaag ccatcgccac cgtttcatcc gcctcgagcg
     2941 cgcccgcgac tttccccacg acctccggcg gaatgagcgg ctcatccccc tgaacgttga
     3001 ccacgatctc ggcgtcgtag gcctccgcgg cctcggccac gcggtccgtg cccgtgcggt
     3061 gctcggacga ggtcatcgcc accttgccgc caaacgccag gacggcatcg cggatgcgct
     3121 cgtcatccgt ggccaccacc acctcggcga tcccgcgagc gcgcgacgcg cgctcgtaca
     3181 cgtgctggat gagcgggcgc ccggcgatct cggcgagcgg cttcccaggc agccgggtcg
     3241 aggcgtagcg cgcaggaata atcacaacgg cattcatcga taacgctcca aaattcgcag
     3301 gacgatctcc gatgttgagc gcccctctac caggggcacg atgcacacct cgcccccaag
     3361 cgcctcgacg agcgccgctt cggggaggtc ttccttccgg tagtcgccgc ccttgacgtg
     3421 cacgtggggc gccaccagcc ggatcagcgc atccggcgtc cgctggggga agatcgccac
     3481 ggcgtcgacg cattcgagcg cggcgagcat ctcggcgcgc tcctcagcgg gtatcagcgg
     3541 gcggttgggg cccttcaacg cgcgcaccga ttcgtcgctg ttcaggccga cgatcaggca
     3601 atcgcccagc gcccgtgcct gctggaggta gcgcgcatgc ccaacatgca agaggtcgaa
     3661 gcacccgttg gtgaagacga tgcgctgccc ttcgcgcttg cgtcggctca cctcgcgcgc
     3721 gagctcatcc caggtgagga ttttcttgcg cgccacgttc actcccccac tcccgaggcg
     3781 cactcctcga tcatcgcgag aagctcatcc ggggtagcca ccgccgtgcc caccttgcgc
     3841 acgaccgcgg cgcccgcgag gtttgccagc gtcgccgctt cctcaaagcc agcgccggcc
     3901 accaatgcga gcgaaagcgt ggcaaacgcc gtgtcgcccg cgccaaccac gtcatacacc
     3961 tccaccggca ccgccggaat cgcacggcag tgcccgtcgt tggagaagag catcatcccc
     4021 cggctgcctc gagtcaccag gagcgcatcc agctccagct ccgagcgcat cacctgcccg
     4081 gcctcgcgca gggccccctc gtcctgcagc cggcggcccg cggccgcttc cgcttccaga
     4141 tggttcaacg taacggccgt agccccgcgc agccgcagca ggttcggcgg tttcgggtt
//
LOCUS       DAOSCV010000328         4198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13333_length_4198_cov_3.704321, whole genome shotgun sequence.
ACCESSION   DAOSCV010000328 DAOSCV010000000
VERSION     DAOSCV010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13333_length_4198_cov_3.704321"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..673
                     /gene="smpB"
                     /locus_tag="PLY56_09065"
     CDS             212..673
                     /gene="smpB"
                     /locus_tag="PLY56_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOJ21671.1"
                     /translation="MREEGKKVVAVNRKARHEYEIEESHEAGIVLTGTEIKSIREGKA
                     NLQDAFARIEGNEVWVHHMHISPYEAGNRYNVDPVRPRKLLLHRREIERLRGKVEQRG
                     LTLIPLQLYLRGGRAKIELGLARGKRLYDRREEIAKRTAARDVERAMRERD"
     gene            806..1831
                     /locus_tag="PLY56_09070"
     CDS             806..1831
                     /locus_tag="PLY56_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21672.1"
                     /translation="MKPNRISAQWVGALILSLAGLLLAGCGGSDDGAKSGADLTEYTF
                     SVVGTAYLEREPATRGLASGDIRLGGATYRVLRRSDSGTSVHQTGTTAPDGSYSLAVD
                     VPVGASPDDLWQLEIFAPGVRIPDANGSTVVANVLLLSTLVPGPGALLSRDANIASTL
                     VAGHLEALYDAGGPVMQITRAQINTLEQRVAELARTPAPGSTPVRLSAVSTHLSTPEA
                     AHASVVSGLAEPVVNVLNSLVSRIRVLNTGVEATSVSIPVGTGTTLIAEPLDSAGNPI
                     PAGVEFLVDTGANSIRVAPSAYNTVILEGVAAGNAGLVARLTIPGTIDIAPKSTRVDV
                     TVLGPGQ"
     gene            2034..2711
                     /locus_tag="PLY56_09075"
     CDS             2034..2711
                     /locus_tag="PLY56_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ21673.1"
                     /translation="MESILVLSDDALWRDALVSELVGAGHRAHASTTSAFLDDPEDQT
                     THLTALVVDLRGASRPHPPLGRVRQAVGNPRVYVLAALSRAQLDHLDFTSGFDDFLVE
                     PLSGQEVTARIRQLRWRANRTDMADLLCAGDLVINLASCEVSLRGVPLALTYQEYELL
                     RYLVEHRGRVFTREQLLSELWGYNYYGGTRTVDVHIRRLRAKLGPEHEELIQTVRNVG
                     YRFRRDA"
     gene            complement(2797..3408)
                     /locus_tag="PLY56_09080"
     CDS             complement(2797..3408)
                     /locus_tag="PLY56_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21674.1"
                     /translation="MNRGNGGTTEGELVFRGGGERALFIVEAGDLARAKRLLAYFDGM
                     ACEVRPIAVGPVVVCAAWVEQPGSGTFESMAEHLRDGYSLSICEPGFSPSMYRVALQL
                     ARDTEGELRPLECCSVCGAPDPFPTTLSLRVGGEEARFLFCQSCSGAVMDAPDAVARL
                     LLGKAAERSPEWRDVELGPPMKSGRAWRFPIRRAADALSPSHR"
BASE COUNT          725 a         1454 c         1313 g          706 t
ORIGIN      
        1 actcccaggg cggcggcatc ggcggctaga cccgacacgt tgggtccacg caccctgtgt
       61 cgcccaatcg gacggcactg acaaccctac cggcgggggg atgctcatcc ccccgccgcg
      121 ttctttttct gggcaacctc gggcccgcgc ggccaccact ctccgaagcc aggtggctat
      181 cccccccaac cgaggcaaag gcacgaagga aatgcgcgaa gaaggcaaga aagtggttgc
      241 ggtcaaccgc aaggcacggc acgaatacga gattgaggaa agccacgagg cgggcattgt
      301 gctcaccgga acggagatca agtcgatccg cgaggggaag gccaacctcc aggatgcctt
      361 cgcacgcatc gaggggaacg aagtgtgggt gcatcacatg cacatcagcc cctacgaggc
      421 gggcaaccgc tacaacgtcg atcccgtccg gccccggaag ctgctgctcc atcgccgcga
      481 gatcgagcgg cttcggggca aagtggagca gcgcggcctc acgctgatcc cgcttcagct
      541 ctacttgcgt ggtggacgcg ccaagatcga gctcggcctg gcgcggggga aacgtctcta
      601 cgaccggcgc gaggagattg ccaagcgcac cgcagcgcgc gatgtcgagc gtgccatgcg
      661 cgagcgcgac tgagcccgcg ctcccccgcc cctggcggcg caccgccatg cctccgtcgc
      721 ggatgatcgc cgtgccgctc agcagcagga aagtgcgtat cgcgacgcga actcagcgca
      781 caggaccgat aagggggaca ctgacttgaa gcccaatcgc atctctgccc agtgggtggg
      841 cgccttgatt ttgtcgcttg ccggccttct cctcgcaggc tgcggcggtt ctgacgatgg
      901 tgcaaaatct ggcgccgacc tcaccgaata cactttttcc gtggtcggta ctgcctacct
      961 cgagcgcgag ccagccaccc gtggccttgc gagcggcgat atccgcctcg gcggtgccac
     1021 ctaccgtgtg ctgcgccgaa gcgactcggg tacctctgtt caccagaccg gcaccaccgc
     1081 gcccgacggc tcctacagcc tagccgtcga tgtgcctgtt ggcgccagcc cggatgatct
     1141 ctggcagctc gagatcttcg ctcccggagt gcgcatcccc gatgccaacg ggagcaccgt
     1201 cgttgccaac gtccttctct tgagcaccct ggtgcctggt cccggggcgt tgctcagccg
     1261 cgatgccaac atcgcgtcca ccctcgtcgc gggtcacctg gaggcgcttt acgacgccgg
     1321 cggccccgtg atgcagatca cgcgcgccca gatcaacacg ctggagcagc gcgtcgctga
     1381 actcgcacgc acgcctgccc cagggtcgac cccggtacgc ctctctgccg tctccactca
     1441 cctctcgacg cccgaggcag cgcatgcgtc cgtggtcagc ggcctggcgg agcctgtggt
     1501 caacgtgctc aatagcctgg tgagccggat tcgggtgctc aatacagggg tggaggccac
     1561 ctcggtatcc atccccgtgg ggactggtac caccctgatc gctgagccgc tcgattccgc
     1621 gggcaacccc attcccgctg gcgtcgagtt tctggtcgat accggcgcca attccatccg
     1681 cgtcgcccct agcgcctaca acacggtgat actcgaaggc gtggctgcgg ggaacgcggg
     1741 ccttgtcgcg cggctcacta tcccgggaac gattgatatc gccccgaaat cgacgcgggt
     1801 cgatgtgacc gtgctcggtc ccgggcaata gccgccacgc tctgccgccc tggtgcattg
     1861 ctggcgtcgc cgcgaagatc ccgcacagga gggcccgcaa agagaggcga taggtagacg
     1921 cccggcgggc cctgtcgttt atcggtcgcc tggctccgta tggtgccgcg tatcttgcct
     1981 acggttggcc agctgcgcca gcatgcaccg cgcggataga gatcctagat tcgatggaga
     2041 gcattctggt cctgagcgat gatgccctct ggcgcgacgc cctcgtctcc gaactagtcg
     2101 gcgcgggcca tcgggcgcac gcatccacca catccgcctt cctggacgat ccggaggacc
     2161 agaccactca cctgaccgcc cttgtcgttg acctgcgcgg cgccagtcgc ccacatcctc
     2221 ctcttgggcg tgtgcgtcag gctgtgggta accccagggt ctacgtgctc gctgccctga
     2281 gccgcgccca gctcgatcac ctcgatttca ccagcgggtt tgatgacttc ctcgtggaac
     2341 ccctctctgg ccaggaagtg acggcacgca tccgccagtt gcggtggcgc gcgaaccgca
     2401 ccgacatggc ggatcttctc tgcgcaggcg atctcgtcat caacctcgcg agctgcgagg
     2461 tctccctgcg tggcgtaccg cttgccctca cttaccagga gtatgagctc ctccgctacc
     2521 tggtggagca ccggggacgc gtcttcacgc gcgagcagct tctcagcgag ctctggggct
     2581 acaactacta tgggggcacg cgcaccgtgg acgtgcacat tcggcgcttg agggccaagc
     2641 taggccccga gcatgaggag ctgatccaga cggttcgcaa tgtgggctac cgcttccgcc
     2701 gggatgcctg aaccgcgcct tccaggccgg aacacgccgg acgagcggat atggcgcgca
     2761 gagtgcgccc tcgggcccat ctggccgggg agacggctat cgatggctcg gcgagagcgc
     2821 atccgccgcg cgacggatcg ggaaccgcca cgcacggccg cttttcatgg gcggccccag
     2881 ctcaacatcg cgccactcag gcgagcgttc cgctgccttg cccaggagca gacgcgccac
     2941 cgcgtctggc gcatccatca ctgcaccgct acagctctgg cagaagagga agcgagcctc
     3001 ttccccgccg acccgcaagg agagcgtggt tgggaaggga tcgggcgcgc cgcacacgct
     3061 gcaacactcc agtggccgca gctctccctc cgtgtcgcgc gcaagctgga gagccacccg
     3121 atacatgctt ggcgagaagc ccggctcgca gatggagagc gagtagccat cgcgcaggtg
     3181 ctccgccatg ctctcgaacg tccccgaccc gggctgttct acccacgcgg cacagaccac
     3241 caccggacca accgcgatcg gacgcacctc gcacgccatg ccgtcgaaat acgccagaag
     3301 ccgcttggca cgcgccagat cccccgcttc gacgatgaag agtgcccttt caccgccacc
     3361 gcggaacacc agctctccct ctgtggtccc accgttccct ctgttcacgg tctcttctcc
     3421 tgacacaatg acgggctatg atagcccatg cccaacagag aagactatag caggagtgtg
     3481 accgcatgtc aacgccccga catctagggg gatcggctgt ccaagccccc cgccgatagg
     3541 gtgctcggcg gaggagcgcc ctttctgggc acgccggaag ggcgcctcgg gagagggatg
     3601 gggatgggcc atgcacgggg tggagccatt ccgatgccgc gaagtatcgg ggatgcccgg
     3661 cccgaatgca ggcgggcaag ggccagcggc acttaaggga gccagtcgcg caggcgttca
     3721 gtcgtctctc atatcactcg gaggagccag gaggggccgg ctatatcccg gcatcctctt
     3781 ccctggcggc aggtggccgt gcatcccggc cgcgttgctt gcccggctcc accgggcagg
     3841 aacgaggcgg tcaggcagat tcgcccaggc acggcaggag ccacacctcg aggaagaacc
     3901 tcacggcgat gaccaccagt gtggccagtc gcatcgtagc ccctttgctt atgcgccccc
     3961 tcgctgcggt tctcacccag gagacgccag aggatgcttt gttggcattc tgactgctga
     4021 agagggccgc gccatcctat gatgacgcgg ccctcgatca attgcgttct gatggactgc
     4081 tactgcccga gcttcaccgg ccggccaaga cgcgccgatt catagacggc gttgaggatt
     4141 tcgaccgtgt gcctggcgct ctccgccgtg atcgccggat ctcggtcttc gcggatgg
//
LOCUS       DAOSCV010000329         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13363_length_4190_cov_4.114148, whole genome shotgun sequence.
ACCESSION   DAOSCV010000329 DAOSCV010000000
VERSION     DAOSCV010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13363_length_4190_cov_4.114148"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLY56_09085"
     CDS             <1..1041
                     /locus_tag="PLY56_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ21675.1"
                     /translation="TLAQVTGRSEDEVITRVERRAQQREEIRQWRDFNRALSSFPHVR
                     ATSAAVEGQGTLTRGEKVTGVRILGADPDAHNRIIRLTEDLIDGRYLGLATEEVVIGY
                     RLAEEMGLALGDRARITSSRGIEQTFTVAGIFDTGQEGIDASWAFVTLRAAQRLFATG
                     TAVTTISLALDDLFAANEVADQVIASLGLEADSWMRENPQALSGLRAQSATAFLISAF
                     SLIAAGFAIASVLIVSVLKRSREIGILKAMGARERQILRVFTLEGLGVALLGATVGAA
                     LGIALILAARQIPQVGVRPGQVPEPLLPGVVRWQTVLGTMGAAIAITILASVFPARRA
                     ARLDPVEVIRGG"
     gene            1034..1714
                     /locus_tag="PLY56_09090"
     CDS             1034..1714
                     /locus_tag="PLY56_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ21676.1"
                     /translation="MAEPIIEAKGIRKVYGGVEPVEVLHGIDLTIRAGEFVAVVGQSG
                     SGKSTLLNILGALDRPTEGTVALEGVPFQELESDDLANLRNCKIGFIFQYHYLLDEFT
                     CLENALMPILIRKGVPDPEDVERVTELLRRVGLGHRLGNRPPALSGGEQQRTAVVRAL
                     ANEPRLVLADEPTGNLDSISGKQVYDLMRSMNRELGIAFVMVTHDDRLAREADRVLRI
                     QDGYLEEA"
     gene            complement(1893..2693)
                     /locus_tag="PLY56_09095"
     CDS             complement(1893..2693)
                     /locus_tag="PLY56_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ21677.1"
                     /translation="MLKGKRILIMGLLDTRSFAWAVGSHAVELGAEVIYTVQNERFRD
                     VLLRRSFKREGLNVDDYNILPCDVTDDDQIAALFERTGPLNGLVHSIGYARPETCLSE
                     NLGDAPREDVLKAFEISAASLAFVAREAQKVLTPGSAIVTLSFNSQQVFPNYNWMGIC
                     KAALEAVAKHLARDLGPLGIRVNCVSAGPQATMASTHIPGFQAIGDAWLQRSPLAWDL
                     NEDRHAVADGVLFLLSDMARRITGEVLYVDSGFHIMGLERQPAKKSDS"
     gene            complement(2871..3305)
                     /locus_tag="PLY56_09100"
     CDS             complement(2871..3305)
                     /locus_tag="PLY56_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21678.1"
                     /translation="MYRSLFIALLLAFPAVATAQSNTASPAAVTVDSAGWLAIGGVSI
                     IQLRDKSVCNSLSDRSSRIMSRMVNALEKIHKKGRFHDINVAVLPPRPGEPSGEPWIA
                     VEGELVMKVTFEDAAANDQKPDKLAAAYAEQFRKALRRIYTP"
     gene            complement(3475..>4190)
                     /locus_tag="PLY56_09105"
     CDS             complement(3475..>4190)
                     /locus_tag="PLY56_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOJ21679.1"
                     /translation="VLVLNGAEPEDPQGNLLLDRLLGARVLFVPEGDRARKAAANAEA
                     ERLRERGERPYVIPTGGSTPLGSLGYALAVQELAEQSEALGIRFRRLFVASGSGGTHA
                     GLHLGARAFLPDLQVMGVAVGEEVEWLRSKVLACAAGAAEAFGIAPAPTAEEITLLGE
                     YAAPGYGVPGPAALEAMRLAARTEGLFVDPVYTAKALSALIDHARTGRLDPEEPVLFW
                     HTGGLPGLFANPAFCLEEA"
BASE COUNT          723 a         1364 c         1403 g          700 t
ORIGIN      
        1 actcttgcac aggtgaccgg gcgttcggag gacgaggtga tcacgcgggt ggagcgccgg
       61 gcacagcagc gcgaggagat ccggcagtgg cgcgacttca accgcgctct cagcagtttt
      121 ccgcacgtgc gcgccacctc ggcggcggtg gaggggcagg gcacgctgac tcgcggcgag
      181 aaggtgaccg gcgtgcgcat cctcggggcg gatccggacg cgcacaaccg catcatccgc
      241 ctcacggagg atctcatcga cggtcgctat ctgggccttg ccactgagga ggtcgtcatc
      301 ggctaccgcc tcgccgagga gatggggctc gccctgggcg accgcgcccg gatcacctcc
      361 agccgcggaa tcgaacagac cttcaccgtc gccggcatct tcgataccgg tcaggagggg
      421 atcgatgcat cctgggcctt tgtcactctt cgcgcggcac agcgtctctt cgccacggga
      481 accgccgtga cgacgatctc cctggcgctg gatgacctct ttgccgccaa cgaggtcgcc
      541 gatcaggtga tcgcttcgct ggggttggag gcggatagct ggatgcgaga gaacccccag
      601 gcgttgagcg gcctgcgcgc gcaatccgca actgcgtttc tcatctcggc gttcagcctg
      661 atcgcggctg gcttcgccat cgccagcgtg ctgatcgtca gcgtcttgaa gcgctcgcgc
      721 gagattggca tcttgaaggc gatgggcgcg cgcgagcgcc agatcctccg agtcttcacc
      781 ttggagggct tgggcgtggc cctcctcggg gccacggtag gcgcggcgct cgggatcgcc
      841 ctgattctgg cggcgcggca gatcccccaa gttggcgtgc gccccggcca ggtgccggag
      901 ccgctcctcc ctggcgtcgt ccggtggcag accgtcctcg gcacgatggg cgccgccatc
      961 gcgatcacga ttctcgccag cgtctttccg gccaggcgcg cggcccgtct cgatccggtg
     1021 gaggtgatcc ggggtggctg agccgatcat cgaggcgaag gggatccgca aggtctatgg
     1081 cggcgtcgaa ccggtcgagg tgctccacgg gattgatctg accatccggg ccggcgaatt
     1141 cgtggccgtg gtgggccaat ccggctccgg caaatcgacg ctcctcaaca tcctcggcgc
     1201 cctcgaccgg cccaccgagg ggaccgtggc cctggaggga gtgccgttcc aggagctgga
     1261 aagcgacgac ctggccaacc tacgcaactg caagatcggt ttcatcttcc agtaccatta
     1321 cctgctcgat gagttcacct gcctggagaa cgccctgatg ccaatcctga ttcgcaaggg
     1381 cgtgccggat ccggaagatg tggagcgcgt gaccgaactg ctgcgccgcg tcggcctggg
     1441 ccatcgtttg ggcaaccgcc cgccggcgct ttccggcggc gagcaacagc gcaccgcggt
     1501 agtacgggcc ctggcgaacg agccgcgctt ggttctcgcc gacgaaccca ccggaaacct
     1561 cgatagcatc agcggcaagc aggtgtacga cctgatgcgc tcgatgaacc gcgagctggg
     1621 gatcgccttc gtcatggtaa cccatgatga ccgtctcgcc cgagaggcgg accgggtgct
     1681 ccggattcaa gatggctacc tagaggaggc gtgagccgcc cactggatgg ctgttacaca
     1741 ggaagtggca ggtggggaag tttctgctcg cgcgcgggtt cgaggcagag agcggcccca
     1801 gggtgggggc ggctctctgc ctctggaagg tggggcaagc cgacgagcgc aggcttactc
     1861 catggaggcc gtcagtgacg gcgtgcctgg ggtcaggagt cgctcttctt ggccggctgg
     1921 cgctccagac ccatgatgtg gaagccgctg tccacgtaga gcacctcgcc ggtgatgcgg
     1981 cgtgccatgt cgctgagcaa gaagaggacg ccatccgcca cggcgtgtcg gtcctcattc
     2041 agatcccagg cgaggggcga gcgctggagc caggcatcgc cgatcgcctg gaagcccggg
     2101 atatgcgtgg acgccatggt ggcctggggg ccggcgctca cgcagttcac gcggatccca
     2161 aggggaccca gatcccgcgc cagatgcttg gcgacggcct ccagggctgc cttgcagatc
     2221 cccatccagt tatagtttgg gaaaacctgc tgcgaattga aggagagagt gacgattgca
     2281 gagcccggtg tgagcacttt ctgggcttcg cgggcgacaa atgccagcga cgccgcgctg
     2341 atctcgaacg ccttgagcac atcctcccgg ggggcatcgc ccaggttctc ggagaggcac
     2401 gtttcagggc gggcataccc gatgctgtgc accaaaccgt tcagcgggcc cgtgcgctca
     2461 aagagcgccg caatctggtc gtcatcggtc acgtcgcagg ggaggatgtt gtagtcgtcg
     2521 acgttcagac cctctcgctt gaacgagcgg cgcagaagca cgtcacggaa gcgctcattc
     2581 tgaacggtgt agatgacctc cgcgccgagt tccaccgcgt ggctaccaac cgcccaggcg
     2641 aagctccgcg tgtcgagcag acccatgatc agaatgcgtt tgcctttgag cacgtgattg
     2701 tcctttcacc gcgtatgggg ccgggagcga ggcgcacaca accccaattc atccttcggc
     2761 ggcggggtgc cggttcctac cgataaggga gatctctatg tcaggatgct acaaagaaac
     2821 agcgcggcct gccgtggtgg cgggccgcgc tgagagaaac catcctgctt ttagggcgtg
     2881 tagatgcggc gcaacgcctt gcggaactgc tccgcgtagg cagccgccaa cttatctggc
     2941 ttctgatcgt tcgcggccgc gtcctcgaaa gtcaccttca tcaccagctc gccttcgacg
     3001 gcgatccagg gctcgcccga gggctctccg ggccggggtg ggagcaccgc cacgttgatg
     3061 tcgtggaagc ggcccttctt gtgaatcttc tcgagtgcgt tcaccatgcg agacatgatg
     3121 cggctcgagc gatcgctcag cgaattgcaa accgacttat cgcgaagctg gatgatggag
     3181 acgccaccaa tcgccagcca tcccgcggag tccacggtga cagccgcagg ggaagccgtg
     3241 ttgctctggg cagtggcaac ggcagggaac gccaagagga gagcgatgaa cagagaccga
     3301 tacatatgag cgtcctccga tttccacgcc aggctcccgc aggcgatgca ggcggccaga
     3361 cgaggtgaaa cctgggagcg gcgcgtgtaa cgccagcact cgtattatag cagcggtgtt
     3421 caggcacgtc aaccctctgc ccggcggcgt ctgccctaca agaggtgttg ccccctacgc
     3481 ctcctccagg cagaaggcag gattggcgaa gagcccgggc aggcctcccg tgtgccagaa
     3541 gaggactggc tcttccgggt cgagacggcc agtacgcgcg tggtcgatca aagccgatag
     3601 cgccttcgcc gtatagacgg gatcgacgaa gaggccctcc gtgcgcgcgg cgaggcgcat
     3661 tgcctccagt gccgccgggc cgggcacgcc atatcccgga gccgcgtatt cacccagaag
     3721 cgtgatctct tccgcggtgg gcgctggcgc gatcccgaac gcctccgccg cgccggccgc
     3781 gcacgccagc accttgctgc gcagccactc cacctcctcg ccaacggcga ctcccatcac
     3841 ctgcaaatcc ggcaggaacg cgcgcgcgcc caggtggagc ccggcgtgcg tgccgcccga
     3901 cccactggcc acgaagaggc gccggaagcg aatccccagc gcctccgact gctcggccag
     3961 ctcttgcact gccagcgcgt agccaaggct gccgaggggt gtggagccgc cggtcggaat
     4021 cacgtacggg cgttcgccac gctcgcggag gcgctcggcc tccgcgttcg ccgcggcctt
     4081 tcgcgcccga tcaccctccg gcacgaagag gacgcgcgct ccaagcagcc ggtccaagag
     4141 aaggttgccc tgcggatctt cgggctccgc gccattgaga acgagcacgg
//
LOCUS       DAOSCV010000330         4171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13445_length_4171_cov_3.071672, whole genome shotgun sequence.
ACCESSION   DAOSCV010000330 DAOSCV010000000
VERSION     DAOSCV010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13445_length_4171_cov_3.071672"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="pilM"
                     /locus_tag="PLY56_09110"
     CDS             complement(<1..556)
                     /gene="pilM"
                     /locus_tag="PLY56_09110"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOJ21680.1"
                     /translation="MVRVTRWVGVDIGTACVKAVVLQRRGGKLEVTGAAKADLPVSDN
                     PTTPPEPAVLAGALRSALGAAVARASDAAVALSSYHFSARVIRMSGAPSDALARAALL
                     EGTRDIPFPADELAGGVHLQAGRDEGESLALVAAARSSLVGAYRKGLEAAGVRANHLG
                     ASSFAAVPATSDPGGGPWVLVDIGA"
     gene            complement(550..1881)
                     /locus_tag="PLY56_09115"
     CDS             complement(550..1881)
                     /locus_tag="PLY56_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOJ21681.1"
                     /translation="MKMPPASLRTTRDARPVRFHTGPVLGEARGSALVMALILLVLLV
                     TLATAAATTVRLRVRAARANAAEVQCTYLAQAGTHLAEAVLRRDDSSVDSREEEWATL
                     GDHGAEAFPLGTGWFRVEIIDAASRVNINAATKEMLMHLPGVDDALSDAILDWRESGE
                     TPRPAGAKADYYQAWPEPYLPREGPFETVGELLLVRDVTPALLYGPPQETGMGEPPSD
                     DRPLSEKVTVLSQERNVNAEGEARVNINEATPEELMGIGDQVLGQAQAAAILQYRQQR
                     GTFSSLGELFSVPGISTDDVRAIVDHVTVTSSTMLPGRVNVNTASEELLAMVPGLTPE
                     TAAAIIQARDGDHGPFESIGELLEPDLLDTEAFSEAVDHLCTRSAAFLVRAMGRVRES
                     RRVVAIEALVERTGESTRIVRWMQVERAPGWIAWGWPRRTADPETGGEIAW"
     gene            complement(1856..2674)
                     /locus_tag="PLY56_09120"
     CDS             complement(1856..2674)
                     /locus_tag="PLY56_09120"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HOJ21682.1"
                     /translation="MSASERGIRVSWRARASCAGKGSARTLHPAPRQGRSEGYTLVEL
                     LVSLVILGTLVGGLGLALSTGLDADERARREGERAQEIRLIINALRADIQHAALTPGS
                     ERSWLIGSDESDGDRDTDTLRLTTRSRRISLGRLELLESSSPAWEETRDADWSAVTYS
                     LAPLDTGEGLGLFRREQTPPGIDPLEELGEEELLSDAVTGFNVRFFDGEAWADAWDTT
                     EEGSGVLPRVIEVTLDLFGGDGEENGVSVTAAFPIRALPPVEATETDEDATSLP"
     gene            complement(2671..3048)
                     /locus_tag="PLY56_09125"
     CDS             complement(2671..3048)
                     /locus_tag="PLY56_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21683.1"
                     /translation="MRGERGFTLIEMIVATLVLAMGIAGALATFGAISRASGMAAEYD
                     QAALLAERRLAEIAVLGVSELTEESGDFGDEAPGWQWEQELLETEIEGVIELRVTVRW
                     ESGNRRREFQVSTYVPEEGAEQS"
     gene            complement(3038..3640)
                     /locus_tag="PLY56_09130"
     CDS             complement(3038..3640)
                     /locus_tag="PLY56_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21684.1"
                     /translation="MRILETGRKRSTRPAGFTVIELCVVLTLLAIAASAVLPAYPQYV
                     RSQRLKATASQLASAIRYASEWSALHEWPVLLSYDEGRHLFTLAAEETDPTEELLARP
                     VPADGEQKEEEIPELDSAWHLLRLPVEVTLEGHEVGEAGSGGPPELRFLPDGRCDDAL
                     FVLGAGDLRRSVRVDGRRGRVVVEDGDALANAPEERADAR"
     gene            complement(3603..4061)
                     /gene="gspG"
                     /locus_tag="PLY56_09135"
     CDS             complement(3603..4061)
                     /gene="gspG"
                     /locus_tag="PLY56_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOJ21685.1"
                     /translation="MLQQRTRRVASAGFTLIELLVVMVILAVLAAAVIPRVVGRTEDA
                     KRARAVADIESLGTALDMYHADNGEYPTTEQGLMALRQAPTSEPVPKGWNGPYLKKAI
                     PLDPWGNEFVYTSPGEHNPESYDLVSYGADGQPGGEGRDADIGNWEEEEY"
BASE COUNT          700 a         1572 c         1219 g          680 t
ORIGIN      
        1 aagccccgat atccacgagt acccagggcc cgccacccgg atcgctcgtc gcgggcaccg
       61 ccgcaaaaga gctggcgccc agatgattgg cgcgcacgcc ggcggcctcc agccctttcc
      121 gataggcacc caccagactc gatcgggccg cggcgacgag cgccagactc tcaccctcat
      181 ctcgccccgc ctgtagatgc acgcctccgg ccagctcatc cgccgggaaa gggatgtcgc
      241 gtgtcccctc caggagagcg gcacgcgcca gggcatccga aggagccccg gacatgcgaa
      301 tgacacgcgc cgagaagtgg taggacgaga gcgccacggc tgcatcgctc gcacgcgcca
      361 cggccgcgcc caacgcggag cgtagcgcgc cggccagtac cgcgggctcg ggaggcgtcg
      421 tcgggttatc actcactggc agatccgctt tcgcggcgcc cgtcacctcc agcttgccgc
      481 cgcggcgctg aaggacgacc gccttcacgc acgccgtgcc gatatctacg cccacccatc
      541 gggtcactct taccacgcta tctctccacc tgtctctgga tccgcggttc gccgcggcca
      601 tccccaggca atccaccctg gggcacgctc tacctgcatc catctcacga tgcgtgtact
      661 ctcgccggtg cgctccacca gcgcctcgat ggcgactacg cggcggctct cccgaacgcg
      721 ccccatcgcg cgcaccagga aggctgccga ccgcgtgcaa agatgatcga ccgcctcgct
      781 gaaggcctcc gtgtcgagga gatctggctc caacagctcg ccgatgctct cgaatgggcc
      841 atgatcgccg tcgcgcgcct gaatgatggc ggcggccgtc tcgggcgtca gccccggaac
      901 catcgccaga agctcttcac tcgccgtatt cacgttcacc cggcctggca gcatggtcga
      961 tgacgtcacc gtcacatgat ccacaatcgc ccgcacatca tccgtgctga tcccaggcac
     1021 cgagaacagc tcgccgagag aggagaacgt gcctcgctgc tggcgatact gcaggatggc
     1081 tgctgcctgc gcctgcccga gcacctggtc gccgattccc atgagttcct ctggcgtcgc
     1141 ctcgtttata ttgacgcgcg cctccccctc ggcgttgaca tttcgctctt gcgaaagtac
     1201 ggttaccttc tcgctcagcg gccggtcgtc agatggcggc tctcccatcc ccgtctcttg
     1261 gggcgggccg tagaggagcg ctggcgtcac atcgcgcacc aacagcagct cgcccaccgt
     1321 ctcgaacggg ccttcccgag gcaggtaggg ctcgggccac gcctggtagt aatccgcctt
     1381 ggcccccgcc ggacgcgggg tctctcccga ctcgcgccag tccaggatcg cgtcggagag
     1441 ggcgtcatcc acccccggga gatgcatcag catctccttg gtcgccgcgt tgatattcac
     1501 ccgagacgct gcgtcgatga tctccacccg gaaccaccct gtcccgagag ggaacgcctc
     1561 cgccccgtga tcacccagcg tcgcccactc ttcctcgcgg gagtccacgc tggaatcatc
     1621 ccgccggagg accgcctccg cgaggtgggt ccccgcctgc gccagatagg tgcactgcac
     1681 ctcggccgcg ttggcgcgcg ccgcgcgcac tctcagccgc accgtggtcg cggcggccgt
     1741 cgcgagcgtc acgaggagga ccaggagaat cagcgccatt accagcgccg agccccgcgc
     1801 ctctcccagc actggtccgg tatggaatcg cacaggccgc gcgtcgcgcg tcgttctaag
     1861 ggaggctggt ggcatcttca tcggtttccg tcgcctcgac agggggcaga gcccggatcg
     1921 ggaacgctgc ggtcaccgaa accccgttct cctcgccatc ccccccgaaa agatcgaggg
     1981 tcacctcgat cacccgcggc agaacgccac tgccctcctc cgtcgtatcc cacgcgtccg
     2041 cccatgcctc gccatcgaaa aagcgcacgt tgaagccggt caccgcgtct gaaagaagct
     2101 cttcctcgcc cagctcctcc aggggatcga tcccaggcgg ggtctgttcc cgccggaata
     2161 accccagccc ctcccccgta tccaagggcg ccagcgagta ggtgaccgcg gaccaatccg
     2221 cgtcgcgtgt ttcctcccac gccggggacg acgactccag cagctccagc cggcccagtg
     2281 atatccggcg actgcgcgtc gtcagccgca gcgtgtccgt atcgcgatcc ccgtcactct
     2341 cgtcgctgcc aatcagccat gagcgttcgg atcccggcgt cagcgcagcg tgctggatat
     2401 cggcgcgcag cgcgttgatg atgaggcgga tctcctgcgc gcgctcgcct tcccgccttg
     2461 ctcgctcatc tgcatccagg cccgtgctca gcgccaggcc caacccgccc accaaagtgc
     2521 ccagaatcac gagcgaaacc agcagctcca cgagcgtgta gccctcagag cgcccctggc
     2581 gaggcgcggg atggagggtt ctcgcactcc ccttgcccgc gcaacttgcc ctagcgcgcc
     2641 aggagactcg tatgccacgc tccgaggcgc tcaactctgc tccgctccct cctcgggcac
     2701 gtaggtggaa acctggaact cgcggcgccg gttaccactc tcccagcgaa ccgtgacccg
     2761 gagctcgatc actccctcga tctcagtctc gagaagctct tgctcccatt gccacccggg
     2821 agcctcgtct ccaaagtcgc ccgactcttc cgtgagctcg gaaacaccca gcaccgcgat
     2881 ctcggccagg cggcgctccg ccagaagcgc cgcctggtca tactcggctg ccatgcccga
     2941 cgcgcggctg atcgcgccga aggtggccag cgccccggcg atccccatgg cgagaaccag
     3001 ggtcgccacg atcatctcga tcagggtgaa accacgctca ccgcgcatcg gcgcgctcct
     3061 ccggcgcatt cgccagcgca tccccgtcct cgaccacgac gcggccacga cggccatcca
     3121 cgcgaacgct ccgccgcagg tcgcccgcgc ccagaacgaa gagggcatcg tcgcatcgcc
     3181 catccggcag gaagcgtaat tccgggggcc cccccgagcc agcttcgcct acctcgtgcc
     3241 cttcgagggt tacctccacg ggcagccgga ggaggtgcca agcgctatcc aattcaggga
     3301 tctcctcctc cttctgctca ccgtcggcag gaaccggtct ggccagcagc tcctcggtag
     3361 gatccgtctc ctccgcagcg agcgtgaaga ggtggcggcc ctcatcatag ctgaggagaa
     3421 ccggccactc gtgaagcgcg ctccactccg aggcgtagcg gatagcggat gccaactgcg
     3481 acgccgtggc tttgaggcgc tggctgcgca catactgcgg ataggccggg agcacggcgg
     3541 atgcggcgat ggcgagcagc gtcagaacga cgcagagctc gatcaccgtg aagccggctg
     3601 gtctagtact cctcttcctc ccagtttcca atatccgcat cgcggccctc tccccctggc
     3661 tgcccatccg cgccatagga gaccaggtcg tacgattcgg ggttgtgctc acccggcgac
     3721 gtatagacaa actcgttgcc ccacgggtcg aggggaatcg ccttcttcag gtagggcccg
     3781 ttccagccct tgggcacggg ctcggaagtg ggggcctggc gcagggccat caacccctgc
     3841 tcagtcgtcg gatactcgcc gttatccgca tggtacatat ccagggcagt gcccaggctc
     3901 tcgatatccg cgacggcgcg cgcgcgcttg gcatcctccg tgcgtcccac cacacgcggg
     3961 attacggctg ccgccaagac ggcaaggatc accatcacca ccagcaactc gatcagggtg
     4021 aaaccagcgg atgcaacccg ccgcgttctt tgctgaagca tgagtcccct ttctacaatg
     4081 gcctgtcccg tgcgcttgca gcaggcgggt tcagaacaac tcccggcccc gcaaacggct
     4141 ctctccctat gagcggctgc tccccgatca c
//
LOCUS       DAOSCV010000331         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13496_length_4158_cov_3.164026, whole genome shotgun sequence.
ACCESSION   DAOSCV010000331 DAOSCV010000000
VERSION     DAOSCV010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13496_length_4158_cov_3.164026"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..793
                     /gene="deoC"
                     /locus_tag="PLY56_09140"
     CDS             68..793
                     /gene="deoC"
                     /locus_tag="PLY56_09140"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ21686.1"
                     /translation="MLNRTDLVKAIDHTVLRPDATLDDVLRACDEAEQYHFASVVVHP
                     YWVPHVERRLANSEVKVGTVVGFPLGATTTRVKVYEMRTVVAMGAQEVQAVINISALK
                     SGDMTRFRRDIEEVLFASHLTGLTQEAQTTLTQFVLETAYLTEREIVLVCELVREIGG
                     EFVVTSTGYAPGGASTNDLRLIRQAIGANMGVKAVGGIRTLDFALDLLNAGANRIGLS
                     ASVPIVEEFIAREEKVPVGSRRR"
     gene            797..1591
                     /gene="recO"
                     /locus_tag="PLY56_09145"
     CDS             797..1591
                     /gene="recO"
                     /locus_tag="PLY56_09145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ21687.1"
                     /translation="MTLRVYTTPVVVLHRTNLGEHDKILTLCTLERGKLNAVAKGARK
                     PISKLAGATELFTHSRVQLAVGRSLDVITQAEITDSFQGIRNDLTRIAHASLMTELVD
                     RFTEERDPHPQLFWLLVNALRALEQTAHPDLVTHCFEMHLLSEVGYGPHLADCAICGT
                     EIHADPAPFSPALGGVLCPAHRRRAADTMPAGLGTLSLGRTLLHISPRREDALEALAA
                     HTSPEIRRGLERLLRAHMQYRLERPLKSPDFIREVTALYTPPSNGR"
     gene            complement(1632..2039)
                     /locus_tag="PLY56_09150"
     CDS             complement(1632..2039)
                     /locus_tag="PLY56_09150"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ21688.1"
                     /translation="MKSQQYRAVVLAALAVAVVAVVAMRRPSGPDASAQAGIPRLLEL
                     GSVSCVPCKMMEPILEELRVEYAGQLQIDFVDVLKETEVARKFDIQIIPTQIFFDAAG
                     KEIYRHVGFLPKEEILAAFQEHGVSLTPPAAAP"
     gene            complement(2036..2269)
                     /locus_tag="PLY56_09155"
     CDS             complement(2036..2269)
                     /locus_tag="PLY56_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013389050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ21689.1"
                     /translation="MTIQILGTGCAKCRTLYANAQQAVAEAGLDAQVEKVEELSAILA
                     FGVMITPALAVDGKVKSAGRVLSPDEIKRILSE"
     gene            complement(2327..>4158)
                     /locus_tag="PLY56_09160"
     CDS             complement(2327..>4158)
                     /locus_tag="PLY56_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ21690.1"
                     /translation="FEGFEEGTGSWRGAGALVTRDTSTAASGKASLRITNTSLAGSFS
                     VTAIGKSFNVGRTPLLRFAYRVPEGVCINPAVITRETPVGIRFTDRHDSYPVTGFIRD
                     AMRDGQWHTAEVNLQQALAGLRFRPDQFDAVQLVFGDRGYTAAPAGASYSLDDFQLIP
                     TASGAAGITLSWSAADVTGIQAYRYKWSDKPDDIPDTVLPGSTTQQTFRGLPEGRLYF
                     HLQAQDGAGNWSEPSHTPFLIDNTPPRLSAPSPAPGKIATSQWTIRLSDTGGSGFDPR
                     SLKMTVNGKEYPLAEFVTTYAHTKRTLTWDWSTATGLFSDPIPDGQAVEMTLAPVKDF
                     AGNAAEPTTFRYTVDRASDKEPPRAPAVDAQGRTLICYDTFTRDVGQWAPYGGGLPGV
                     TLHLDPERGDTCARLSTSGSSYGYWIRMSAFDAVKAPFISFDYRCPQTVNLHLALHIK
                     GRWYTIPLSGKNPTHPNLGPVKGFNPDGKWHTIAFNYLDILRAAKMGGESVPITHLAL
                     WHQGSGGGQFYDIDNFQIFGSGTPEFEVTWSAADPTGIHAFSYRIDADPEAGLAEQGG
                     TETTERKAKITGLKPGMWFLRVRARDGAGNWSAPALVTWHVPG"
BASE COUNT          739 a         1324 c         1348 g          747 t
ORIGIN      
        1 agcgcgatac gacggtgtaa ggctcacgac gccggggaac cacgaagcgg gaggtgagtc
       61 tctcgcgatg ctaaatcgaa cggatctggt gaaggcgatc gaccacaccg tcctgcgccc
      121 ggatgcgacg ctcgacgatg tgttgcgggc ctgcgacgag gcggaacaat accacttcgc
      181 atccgtggtg gtccatccct actgggtgcc gcacgtggaa cggcgtctcg caaacagcga
      241 ggtcaaggtg ggaacggtgg tgggcttccc ccttggggcg accacgaccc gcgtcaaggt
      301 gtatgagatg cgaaccgtgg tggcgatggg cgcccaggag gtgcaggcgg taatcaacat
      361 cagcgccctc aagtcgggcg atatgacccg cttccggcgt gacattgagg aggttctgtt
      421 cgcgtctcac ctgacaggtc tgacccagga ggcgcagacg acgttgacgc agttcgtgct
      481 ggagacggcc tacctcactg agagggagat cgtactcgtt tgcgagttgg tgcgcgagat
      541 tggtggcgag ttcgtggtga cctccaccgg ctacgctccc ggaggggcgt caacgaatga
      601 ccttcgcctg attcgccagg cgattggggc caacatgggg gtcaaagcgg tcgggggaat
      661 ccgaacgctc gattttgccc tcgatctctt gaacgccggc gccaatcgga tcggcctatc
      721 ggcgagcgtg cctattgttg aggagttcat cgcgcgtgag gagaaggtgc ctgttggatc
      781 gagacggaga taggcgttga cgctgcgcgt atacaccacg ccggtcgtcg tgctgcaccg
      841 gaccaacctg ggcgagcatg acaagatact taccctctgc acgttggagc gcggcaagct
      901 gaacgcggtg gcgaaggggg cgcgcaagcc aatcagcaag ctcgcgggcg ccaccgagct
      961 cttcacgcat agccgggttc agcttgccgt tggccgcagt ctcgacgtga tcacccaggc
     1021 ggagatcacc gactctttcc aggggattcg caacgacctc acgcggatcg cgcatgcctc
     1081 gctcatgacg gagttggtgg atcgctttac cgaagagcgc gacccgcatc ctcagctctt
     1141 ctggctcctg gtgaacgcgc tgcgggcgct agagcaaacc gcgcacccgg acctggtgac
     1201 gcattgcttc gagatgcatc tcctctcgga ggttggctac gggccgcacc tggccgattg
     1261 cgccatctgc ggcacggaga tccacgcaga tcctgcgcca ttcagccccg cgctgggggg
     1321 cgtgctctgc ccggcgcatc gccggcgcgc cgccgatacc atgccagcgg gcctgggaac
     1381 gctctccctg gggcgaacac tcctccacat ctcgcccagg cgcgaggatg cactcgaagc
     1441 cctcgccgct catacatcgc cggaaatccg gcgcgggctg gaacgcctcc tgcgtgcgca
     1501 catgcagtat cgcctggaga ggcccctgaa aagcccagat ttcattcgcg aggtgacggc
     1561 gctctatacc cccccgagca atgggcgctg aggcgcatat ccgggactac ttcaatgcga
     1621 ccgcgcacag ctcacggggc cgccgcgggc ggcgtcagag agacgccatg ctcctggaag
     1681 gctgccagga tctcttcctt cggcaagaac ccgacgtgcc ggtagatctc tttccccgcg
     1741 gcatcgaaga agatctgcgt cgggatgatc tggatatcga actttcgagc gacctctgtc
     1801 tccttcaaca catcaacgaa gtcgatctgc agctgcccgg cgtactcgac gcgcagctcc
     1861 tcaagaatcg gctccatcat cttgcacgga acgcagctga ccgatccaag ctcgagcagg
     1921 cggggaatac ctgcctgcgc agaggcgtcg ggcccggacg gccggcgcat agccacgact
     1981 gcgaccacag cgacagcgag cgcggccagc acaacggcgc ggtactgctg gctcttcatt
     2041 cgctgagtat cctcttgatc tcgtccggcg atagcacccg cccggcggat ttaaccttgc
     2101 cgtccaccgc cagcgcgggc gtgatcatca cgccaaacgc taggatggcg gagagctctt
     2161 ccaccttctc cacctgggca tccagccctg cctccgcgac tgcttgctgc gcgttggcat
     2221 acagcgtccg gcacttggcg caacccgttc ccaagatctg aatcgtcatc tgccacgttc
     2281 tcctctcgca gctctgctgg cgctcccatc atagcctagc gcgcctctat ccgggtacat
     2341 gccaggtgac aagggcgggg gcgctccagt tgccggcgcc atcgcgggcg cgcacgcgca
     2401 ggaaccacat tcccggcttc aggccggtga tcttcgcctt gcgctcggtg gtctcggtac
     2461 caccctgctc ggcgaggccc gcctctgggt cggcatcgat tcgatagctg aaggcgtgga
     2521 tcccagtggg atccgcggca ctccaggtga cttcgaactc tggagttccg gagccgaaga
     2581 tctggaagtt gtcgatatcg tagaactgcc ctccgccgct cccctggtgc cacagggcga
     2641 ggtgggtgat cggaacgctc tcgcccccca tcttggcggc gcgtaagata tcgaggtagt
     2701 tgaacgcgat ggtgtgccac ttgccgtccg gattgaaccc cttcactggg cccaggttcg
     2761 ggtgggtcgg gttcttgcca ctgagcggta tggtgtacca tcgccccttg atatggagcg
     2821 cgaggtggag gttcaccgtc tgcgggcaac gatagtcaaa cgagatgaac ggcgccttca
     2881 ccgcatcgaa ggcgctcatg cggatccagt agccgtaaga gctgcccgat gtcgacaggc
     2941 gcgcgcacgt gtcgccccgt tccgggtcca gatggagcgt gacgccgggc aatccgccgc
     3001 cgtacggtgc ccactggccc acatcgcgag tgaaggtgtc gtagcagatc agggtgcgcc
     3061 cctgcgcgtc cactgccggg gcgcgtggcg gctccttgtc cgacgcgcgg tccacggtgt
     3121 agcggaaggt ggtcggctcc gcagcgttcc ccgcgaaatc cttgaccggc gccagcgtca
     3181 tttccaccgc ctgcccatcg gggatcggat ccgagaagag tccggttgcg gtggaccaat
     3241 cccacgtcag cgtcctcttg gtgtgggcat acgtggtgac gaactccgcc agcggatact
     3301 ctttgccgtt cacggtcatc ttcagcgacc gaggatcgaa cccggagcca ccggtgtcgc
     3361 tgaggcggat cgtccactgc gacgtggcga ttttgccggg cgcgggcgag ggcgcgctaa
     3421 ggcgcggcgg cgtgttatcg atcaggaaag gcgtatgcga gggctcgctc cagttgccag
     3481 cgccgtcctg agcctgcaga tggaagtaca gccgcccctc cgggagaccc cggaacgtct
     3541 gctgggtggt gctgccaggg agcaccgtgt ccgggatatc atccggcttg tcgctccact
     3601 tatagcggta agcctgaatc ccggtcacgt ccgcggcaga ccaggagagc gtgatccccg
     3661 cggcaccact cgccgtcggg atcagctgga aatcatccag cgagtaggac gcgcccgcgg
     3721 gcgctgcggt gtagccgcga tcaccaaaca caagctggac ggcgtcgaac tggtctggcc
     3781 ggaagcgcag gcctgccagc gcctgctgga ggttgacctc agccgtatgc cattggccat
     3841 cgcgcatcgc gtcgcgaatg aagccggtga ccggatagct atcgtgccgg tcggtaaagc
     3901 ggatgccgac cggggtttcg cgcgtgatga ccgccgggtt gatgcaaacc ccctcgggaa
     3961 cacggtaagc aaagcggagg aggggagtgc gcccaacatt gaagctcttc ccgatagcag
     4021 tgaccgagaa cgagccggcg agactggtgt tggtgatccg caggctcgct tttccgctgg
     4081 ctgcggtaga ggtatctcgg gtgacaagag cgcctgcccc cctccaggac ccggtgccct
     4141 cttcgaagcc ctcgaaga
//
LOCUS       DAOSCV010000332         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13508_length_4156_cov_4.005852, whole genome shotgun sequence.
ACCESSION   DAOSCV010000332 DAOSCV010000000
VERSION     DAOSCV010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13508_length_4156_cov_4.005852"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLY56_09165"
     CDS             complement(<1..283)
                     /locus_tag="PLY56_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21691.1"
                     /translation="MLVVDDEPAITDPIQAALQMEGYQVGVRTTAAACLAAVRTWRPD
                     LILLDIMLPGGMTGLDALRIIRQETDLPVILITGRSEDAYKVLGLEYADD"
     gene            complement(550..714)
                     /locus_tag="PLY56_09170"
     CDS             complement(550..714)
                     /locus_tag="PLY56_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21692.1"
                     /translation="MSATSGGVGAFELPQRVLLRADEQGRFVALGNRVNLTRLLEMEC
                     QRASREDAPG"
     gene            1024..1443
                     /locus_tag="PLY56_09175"
     CDS             1024..1443
                     /locus_tag="PLY56_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ21693.1"
                     /translation="MKSYREELWFNVPQRRAFINITPQVEECLRKSGIREGLLLCNAM
                     HITASVFINDDERGLHHDYEEWLENLAPHEPISRYWHNRTGEDNGDAHLKRQVMGREV
                     VVAITNGKLDFGPWEQIFYGEFDGGRRKRVLVKIIGE"
     gene            1661..1978
                     /gene="sugE"
                     /locus_tag="PLY56_09180"
     CDS             1661..1978
                     /gene="sugE"
                     /locus_tag="PLY56_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HOJ21694.1"
                     /translation="MAWVILFLAGLFEVAWAVGLKSTAGFTKFWPSLVTLAFMAASVF
                     LLGLAVRDLPVGTAYAVWTGIGAIGTVIFGILVLGEPRHAIRLVCLALIAAGIIGLKV
                     TSA"
     gene            complement(2209..2610)
                     /locus_tag="PLY56_09185"
     CDS             complement(2209..2610)
                     /locus_tag="PLY56_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOJ21695.1"
                     /translation="MARRLFVGGLSFDTSAAELRKAFEAVGPVVAADVVMDRETGRSR
                     GFGFVEMANDEDAATAVAQLNGTTLGGRTLTVNEASARSERGGGGRSGAGRGGGPGRG
                     RGEREQRSRSVEGGRAGRIPGAGGYGGKNSW"
     gene            complement(2906..3763)
                     /locus_tag="PLY56_09190"
                     /pseudo
     CDS             complement(2906..3763)
                     /locus_tag="PLY56_09190"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT          721 a         1420 c         1276 g          739 t
ORIGIN      
        1 agtcatctgc gtattcaagg cctaatactt tgtaggcgtc ctcgctgcgc ccggtaatca
       61 agatgaccgg caggtccgtc tcctggcgta tgatgcgcaa ggcatccaaa ccggtcatcc
      121 ccccaggaag catgatgtcg agcagtatca gatccggacg ccaggtgcgc accgcagcca
      181 gacaggccgc cgcggtggtg cgcacaccca cctgatagcc ttccatctgc agcgcagcct
      241 gaatcggatc cgtgattgct ggctcatcat caacgacaag caccttcatc ggttatacag
      301 tcggccttcc taacaccaca gcgatgcttc cacttggtat gcgatctctc caggcgattc
      361 ctctggcggc cacgggtctg actcctcccc ctggcccctc cgcagagggc cgcccagggc
      421 tactcccgta tctctactcg aacgcccgaa ccctcccttg cccggcgaag accggctgga
      481 ccgacgcgcg gaaggctctg gctcgcggaa gccaagaggc ccggaaggaa accgatcgtg
      541 tctccccgct catccgggag catcctcgcg tgaggcacgc tggcactcca tctcgagcag
      601 tcttgtcagg ttcacccggt tgccgagagc gacgaagcga ccctgctcat ccgctctgag
      661 gagtacccgc tggggaagct cgaatgcgcc aacgccgcca ctcgtcgcgc tcatggccgg
      721 cgcgctcgcc tttcgtgcga tccgcgcccc ccggcaacca tgccgcctac gtcgatgctg
      781 gctttggtct tcgctgcctg cattaccgat attctcccgt tcgccgaggc agcggcagcc
      841 tcctgcggga tgacggcacc acaagagttg gacgctaact gtttggtcgc ttagcaggca
      901 gccgggaggg cgctagagga gaggatcgcg ggcgaatggg gcagcagcgc cctttcggct
      961 ccgcggcgcg tagggcacgg gcaggccgtt gcgtatcggg taggttcatg gtatcataca
     1021 ggcatgaaga gctaccgtga ggaactgtgg ttcaacgtcc cgcagcggcg ggcattcatc
     1081 aacatcacgc cccaggtgga ggagtgcctg cgcaagagtg gcatccgcga gggcctgctg
     1141 ctttgtaacg ccatgcacat caccgcctct gtcttcatta acgacgacga gcggggcctg
     1201 caccacgatt acgaggagtg gctggagaac ctggcccctc acgagcctat cagccggtac
     1261 tggcacaacc gcacgggcga ggacaacggc gacgctcacc tgaagcgcca ggtgatgggc
     1321 cgggaggtgg ttgtcgccat caccaacggc aagctcgatt ttggcccctg ggagcagatc
     1381 ttctacggcg agtttgatgg gggccggcgc aagcgcgtgc tggtgaagat catcggcgaa
     1441 taggcggggc ccgctcctcg ctgaagtgcc ttgatcgttt ggcagcgtgg cttcacactc
     1501 tgaccctgct cgaaatggcc ctgagactga gagtgctgat gagacggtgc gacactgatt
     1561 tcagtgtcgc caaccagccg gccttttggc cccgttggga cgacccagcg tgcgctgcta
     1621 tcgcgaaggg gacgccccct tcacaccgga ggatcggtgc atggcgtggg tgattctttt
     1681 tctagcgggg ctgttcgagg tcgcttgggc cgttggcctg aaatcgacgg ctggcttcac
     1741 taagttctgg ccctctctgg tcacgcttgc cttcatggcg gcaagcgtct ttctcctcgg
     1801 gcttgccgtg cgagatctgc cggtcggcac ggcttacgcc gtgtggacgg ggattggggc
     1861 cattggcacc gtgatcttcg gcatcctcgt gttgggcgag ccccgccacg cgatccggct
     1921 cgtgtgcctc gcgctgattg cagcggggat catcggactc aaggtcacct ctgcctagga
     1981 gaagcgggtg actaacaggc catccgtcat cacctgatcc gggtcgctgg caggtcgcga
     2041 gcaacaaccg gacaggctcc aggcccgaag ggccgtgcat gcaacgacgc ctccgcgcgg
     2101 cgctcctggg gaacgcgcgt tccctgaccc aagccgcatg gagaggcacg cgggagggag
     2161 atccctgctg gcacgccagg ccttcatgcg gtagccggga gtgccggcct accaggagtt
     2221 cttgccgccg tagccgccgg caccgggaat gcgcccggcg cggcccccct cgacactgcg
     2281 actgcgttgt tcacgctcgc cacgcccgcg gccaggcccc cctccacggc cggcgccgct
     2341 ccgacctccg cctccgcgct cggatcgcgc cgacgcctcg ttcaccgtca gcgtgcgccc
     2401 acccaaagtg gtcccattga gctgagcgac agcggttgcc gcatcctcgt cgtttgccat
     2461 ctccacgaag ccaaatcccc ggctccgccc cgtttcccgg tccatcacca catccgccgc
     2521 gacgaccggc cccactgcct caaacgcctt ccgaagctcc gccgcgctcg tatcaaagga
     2581 aagaccaccc acgaacagcc gtcttgccac ttgcggctcc tttcacggcc catcactctt
     2641 gacgcggatg gaaagccggc cccgatgcgg actgcgcttt ccgcatgcga ttgcccgcag
     2701 accacgggcg atcccgcata cccccattat acccacttgc gttgcgctcg aatgcagcag
     2761 gcgacgctcc accggctctt gctttcgttc gcggcagcca ccagccagct cacagggcgt
     2821 ccggtcgccg gcaccggcgg cccggcctct ctccctctcc gcgcgcggca ttccgccgtt
     2881 gccacgccgg tgcgccttgc acattctatc tcacgcccgg gatgagccgg gcggcgttct
     2941 ccgcgtacac cttgcgcagg acatcatcgg gcaggaaaat gccgtagatg cgccatcggc
     3001 ccttctgggg gcgcggtccg atagcgtatg ggaagtactc gtcatcggtt tcgagaaagc
     3061 gaaagagcac gcggtaatgc cacgcttctg gctcgaaatc ggcgccgaac aggatgcgat
     3121 ccgcgtgccg gaggaagaag cgccgcgcgg tgtagggctg ccggcccagc tcctcgtaac
     3181 gcgcggagag gtccacgttc aagttggggc aggcatccag aaggtcgctc acccatctca
     3241 ggttctccac ataggagcag agatgcgccg agatgaaggt ggtgttcgga tgtcggcgca
     3301 ccaggcgcgc ctgggcttcc atcagcacct cgaactgggg gaaatcgccc ccgtagaagt
     3361 gccagtcggg gtgtcacgcc agctgctcca agcgttcgtt gcggccatcc agcggccgga
     3421 agaaggcgcg tggatccgca acgtggtaga gcacaggaat gcccaactca cccgcggctg
     3481 cgaagatagg cgcaaggcgc tcatcatccg gcatgagcag ccttccctgg ggatcccgaa
     3541 tccgcaggcc gaggctcttg tggatcttca cgccctgggc cccgcgcgcg acggcctcac
     3601 gcaagtcggc cgccatccgc tccccgaagc cctcgccctc ctcaagccgc ttccagttga
     3661 gggtatggaa caggatgaag cgctccgggt agggctcttt caggaggcgc aagcactctt
     3721 ccagggagac cgcgctgccg taacccgcat ccagattgac catcgcccgc aggccgaggc
     3781 tgtccatcag atccaacagt cgggagacag cctcggcccc ctggcgcacg atccctccca
     3841 aggtggttgt gcgcatcgat cgccgggaac cgggggcggt cgacctggtg ttccggcagg
     3901 accagctccg actccggcag gaaatccttc agcttccagt catcgatact ctgcggcagc
     3961 atgagttgcc ctccggacaa agcggctagt cctatcgcgc gccccccagt cgggtcacca
     4021 tgcgctctgg atgccagccg gccgctcggt gctgctggca gccggcccct cagaactcca
     4081 ccgtcatgcc atcataggcg agggtgatcc ccttctcggc aacagcagca gtttccgcag
     4141 cgaatggggc gtatga
//
LOCUS       DAOSCV010000333         4135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13594_length_4135_cov_2.704902, whole genome shotgun sequence.
ACCESSION   DAOSCV010000333 DAOSCV010000000
VERSION     DAOSCV010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13594_length_4135_cov_2.704902"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLY56_09195"
     CDS             <1..247
                     /locus_tag="PLY56_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ21696.1"
                     /translation="PAWLALMTELAGDEHRGGVVGIMNTGQGIGAALGAAVGGSLYDY
                     LGPRAPFLASALLFTVSVLVVFRYVRRVPARRQVGIR"
     gene            292..1464
                     /locus_tag="PLY56_09200"
     CDS             292..1464
                     /locus_tag="PLY56_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOJ21697.1"
                     /translation="MAGAFALTFPWIVLRLRHWHETADPLAVAAISGLAILGAAFILS
                     WAAEVAQMDISQSLALAFLALISILPEYAVDMYFAWQAGKNPEYMGYATANMTGANRL
                     LIGLGWSAVVLLYWLRSRKTTVTLEPGQSTEMTFLALATIWSFTIPLRHEIGMIDLVV
                     LLTIFVLYMWQASKAEHDEPEFTGPPLALSLLPKARRRMAVVGFFIWAAAIILASAEP
                     FSEGLVETGKRWGVDEFLLVQWLAPLASESPEFIVASLFALRGTPSLGLGALISSKVN
                     QWTLLVAMLPLVFSISAGRPGGLILDARQEEEMLLTSAQSLFALAVILNFRMSIWEAF
                     GLIFLFVTQLFFTDTHIRYAYAFAYIGLTVIVIARHPDRLRNLWSLVRCGGHRSEG"
     gene            complement(1564..2982)
                     /gene="selA"
                     /locus_tag="PLY56_09205"
     CDS             complement(1564..2982)
                     /gene="selA"
                     /locus_tag="PLY56_09205"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOJ21698.1"
                     /translation="MRERLREIPAVDEILRHERVVCLLEQTPRPLAVDAIRLVLDGLR
                     QRIRHGEEVDTSLPAVAEEAAGAIVQRGRRSLRRAINATGVLLNTGLGRAVLAPQAVE
                     AVCEVAGSHSVLEVEIETGRRGSRLAPVERLLRALTGAPAATVVNNNAAAVLLALNTL
                     AAGREVIISRGQLVEIGGQFRMPDVIHASGCRMVEVGTTNKTHLYDYEKAITPETALL
                     LHVHPSNYRIVGFAEQVPLPDLVALGHQRGILVVSDLGSGSFFDVSRAGLSPEPTVPR
                     IVATGVDLVTFSGDKLLGGPQAGLLLGGEEVIRRITRNPLMRALRCDKLTLAALEATL
                     RLYLDEKSALQQIPILRALTRSLEEIEALAQQVAREAARPGVKLSVVATTCEVGGGSL
                     PAEELPSFAVRVEAAGCSPEEVARSLRGGDPAIFGRIARDAFLLDMRAVSDQEADEVI
                     SALSRLAGPEVEPDAGTKEASS"
     gene            3246..3860
                     /gene="lepB"
                     /locus_tag="PLY56_09210"
     CDS             3246..3860
                     /gene="lepB"
                     /locus_tag="PLY56_09210"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ21699.1"
                     /translation="MPRGRTGGAASGFGKGKLRVSNPRYHTAWWRVFETVVLIAALAG
                     AAYLRLHVLLPARIISQSMVPTLQVDDYVLINRLAYRERLPKPGEIVAFNTLDRQQVW
                     VKRAVAGPDDRVELRAGKLYRNGQPVDESYIVQRRRISFPPVRVPPDHIFVLGDNRDN
                     SDDSRYWGPLHRQYVVGKVVWVYWPWERKGRPGTPPIEANRGAE"
     gene            3903..>4135
                     /locus_tag="PLY56_09215"
     CDS             3903..>4135
                     /locus_tag="PLY56_09215"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ21700.1"
                     /translation="MMWRGVLCLCLVLGLGAPAAPEYARPEDHGGLRDIALIYRASAP
                     RTVEELLPLVAYVDVAGQPNAWFFDGFLFLTQQP"
BASE COUNT          646 a         1359 c         1345 g          785 t
ORIGIN      
        1 gcctgcgtgg ctggccctga tgactgagct cgcgggggac gagcaccgtg gaggcgtggt
       61 cgggattatg aacacggggc aggggatcgg ggcggctctg ggtgccgccg tgggcggcag
      121 cctgtatgac tacctgggac cccgcgcgcc cttcctggcc agcgcgcttc ttttcacggt
      181 ctcggtcctg gttgtgtttc gatatgtgcg cagggttccg gcgcgtagac aggtgggtat
      241 ccgttgagca ccaagataac taagcgaaac ttaactggtc actggcttct gatggctggc
      301 gcgttcgcgc tgacgtttcc gtggattgtc ctgcggcttc ggcactggca tgagacggcc
      361 gatcccctgg cggtggccgc catctccggc ctggcgatcc tcggtgccgc gttcatcctc
      421 tcgtgggcgg ccgaggtggc gcagatggac atttcgcagt cactcgcgct cgccttcctg
      481 gcgctgatct ccatcctgcc cgaatacgcc gtggatatgt acttcgcctg gcaggcgggg
      541 aaaaaccccg agtatatggg gtatgccacc gcgaatatga cgggcgccaa ccgcttgctc
      601 atcggcctgg gatggtcggc ggtcgtgctc ctctactggc tgcgcagccg caagacgacc
      661 gtcacgctgg agccggggca atccacggag atgactttcc tggcgctggc cacgatctgg
      721 tcgttcacca tcccgttgcg acatgagatc gggatgatcg acctggtcgt tttgctcacc
      781 atttttgtgc tctatatgtg gcaggcctcc aaggcggagc acgatgagcc ggagttcact
      841 ggcccgccgt tggcgctctc ccttctcccg aaagcccggc ggcggatggc ggtggttggg
      901 ttcttcatat gggcggcggc gatcatcctc gcctcggcag agccattctc ggagggcctc
      961 gttgaaacgg ggaagcgctg gggtgtggat gagttcctac tggtacaatg gctggcgccg
     1021 cttgcctcgg aatcgcccga attcatcgtg gcctcgctct ttgccttgcg cggcactccc
     1081 agcctcggcc tgggcgcgct gatctcatcg aaggtcaacc agtggacgct cctcgtcgcg
     1141 atgctccctc tggtcttcag catttcggcg ggccggccgg gcgggctgat cctggacgcg
     1201 cgccaggagg aggagatgtt gctcacctcg gcgcagtcgc tcttcgcgct ggcggtgatt
     1261 ctcaacttcc gaatgtccat ctgggaggcg ttcgggctga tcttcctctt cgtcacccag
     1321 ctcttcttca cggatacaca tatccgctat gcctacgcct tcgcctacat cggcctcaca
     1381 gtcattgtga ttgcccggca tccagatcgc ctgcgcaatc tgtggagcct ggtgcgctgt
     1441 ggtggccatc gctcggaagg atagcgaggc tttcatccgg tatcgggaac aagcgcgcgt
     1501 ggggaacact catcagtgct ccccacgcgc acgggatctc aaattctaac gccggaatcc
     1561 ctctcacgaa gacgcttcct tggttcccgc gtccggctct acttccggcc cggccaggcg
     1621 tgacagcgcg ctgatcactt cgtcggcttc ctgatccgac acagcacgca tatcgagcag
     1681 aaacgcatcg cgagcgatcc gcccaaagat tgccgggtct cccccgcgta gcgaacgcgc
     1741 gacctcctcg ggagagcaac cggcagcctc aacgcgtacg gcgaagctgg gaagctcttc
     1801 cgcgggcaga gatccaccgc cgacctcgca ggtagtcgcc acgacgctaa gcttcacgcc
     1861 cgggcgcgcg gcctcgcgtg cgacctgctg ggcgagcgcc tcgatctctt ccagagagcg
     1921 cgtgagcgcg cgcaggatgg ggatctgctg gagggcgctc ttctcgtcta ggtagaggcg
     1981 aagcgtcgct tcgagcgccg ccagggtgag cttatcgcac cggagcgcgc gcatcagcgg
     2041 gttgcgcgtg atccgtcgga tcacctcctc gccgccgagg agcagccctg cttgaggacc
     2101 gcccaggagc ttgtcgccgc tgaaggtgac gaggtcgacg cccgtcgcga cgattcgggg
     2161 cacggtgggc tcgggggaga gcccggcgcg cgatacgtcg aaaaacgagc cgcttccgag
     2221 gtcgctgact accaggatcc cgcgctggtg tccgagggca acgagatcgg gaagagggac
     2281 ctgctcggca aaaccgacga tccggtagtt gctcgggtgc acgtggagca ggagcgccgt
     2341 ctcgggggtg atcgcctttt cgtaatcgta gaggtgcgtc ttgttggtgg tacccacttc
     2401 caccatgcgg cacccgctcg catggatcac gtccggcatc cggaactggc cgccgatctc
     2461 cacgagctgc ccgcgcgaga tgatcacctc gcgcccggcg gcgagcgtat tcagcgccag
     2521 gaggaccgcc gcggcgttgt tgttcaccac ggtggccgcc ggcgcccccg tgagcgcgcg
     2581 taacagccgc tcgaccggcg cgagccgtga cccacggcgt cccgtctcga tctcgacctc
     2641 cagaaccgag tgcgaccccg cgacttcgca caccgcctcg accgcctgcg gtgccaggac
     2701 agcccggccc aggccggtat tcaaaagaac gcccgtggcg ttgatcgcgc gccgcaggga
     2761 ccgccggccg cgctggacga tggcacctgc agcctcctcg gccaccgccg gcaacgaggt
     2821 gtctacctcc tcgccatgcc ggatgcgctg gcggaggccg tccagcacca accggatcgc
     2881 gtctaccgcg agcgggcgcg gcgtctgttc caacaggcac actacccgtt catggcgcag
     2941 gatctcatcc accgcgggga tctcgcggag gcgttctctc atcaccgaat cttccatgtg
     3001 gattgacttc tggaatcgcg ggcgcggctt gcctaaccct cggccccttc cctaccctcc
     3061 gcccccgagc cagccccagg gaaggggagc gaccgcgtgg acgtcgtgga cggtgccgga
     3121 tggatcgcgc actccgggca tccatacctg ccgcgctcat ccgtggtagc gatgcccgcg
     3181 agcgttgtgt tgcgatccct gttcggctcg cgtgtggttg acacctcccc gcgggcggga
     3241 gtacaatgcc gagagggcgc acgggcggcg ccgcgtccgg ctttgggaaa gggaaactga
     3301 gggtgagcaa cccgcgctat cataccgcct ggtggcgggt ctttgaaacg gtggtcctga
     3361 ttgccgccct ggcaggagcc gcttacctgc gtcttcacgt cctccttccc gcgcgcatca
     3421 tttcccagtc gatggttccc acgcttcaag tggatgacta cgtcctgatc aaccgcctgg
     3481 cctaccggga acgcctcccc aagccgggcg agatcgtcgc gttcaacacg ctggaccgcc
     3541 agcaggtctg ggtcaagcgc gcggttgcgg gtccggatga ccgcgtcgag ctacgcgccg
     3601 gcaagctcta ccgcaacggc cagccggtgg atgagtcgta catcgtgcag cgccggcgaa
     3661 tcagcttccc tcccgtgcgc gttcccccag atcacatctt cgttttgggc gataaccgcg
     3721 acaatagcga cgacagccga tactggggcc cgctgcatcg gcagtacgtg gtgggcaagg
     3781 tggtgtgggt ctactggccc tgggagcgca agggccgtcc cgggacgcct ccgatagagg
     3841 caaaccgcgg cgcggagtga gaccgaaact gcttcaacac tcgttaatca cccaggagga
     3901 tcatgatgtg gcgaggggtc ctctgcctct gtctcgttct tgggttaggg gcgccggcgg
     3961 cgccggagta tgcgcgcccg gaggatcatg gcggcctgcg tgacatcgcc ctgatctacc
     4021 gagcgagcgc gcctcgaacg gtggaggagt tgcttcccct ggtcgcctat gtcgatgtgg
     4081 ccggccagcc gaatgcgtgg ttctttgacg gcttcctctt cctcactcag cagcc
//
LOCUS       DAOSCV010000334         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13636_length_4124_cov_3.776112, whole genome shotgun sequence.
ACCESSION   DAOSCV010000334 DAOSCV010000000
VERSION     DAOSCV010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13636_length_4124_cov_3.776112"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1184)
                     /locus_tag="PLY56_09220"
     CDS             complement(162..1184)
                     /locus_tag="PLY56_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ21701.1"
                     /translation="MIKGLILAAGRGTRLRPLTDRRPKPLVPIAGRPILQYNIENLRD
                     AGIDDIVIVVNPDSDEVERYFGDGSRIGVRLTYVAQHELRGTAHAVYQARDAIGDSPF
                     ILVFGDNMTGYRLRDLAASLEKYSGGSVLAVCAGGDHRKHAVVCVDGDRVCLIEERPE
                     IPRGPYTCAGMYAFTPSIFPAIEKIQPVANGEYYLPHAIQELINDGQPVYYCLAEGWR
                     VNINTPADMMQALAYVVRDMADGYRHALRRGAAPEVLLGANVHLDENVSLGGCSYIGS
                     GCRIGSNSYIGNSMLMENVLVGSDCVVHNAILGQGVVVPDGTRALGDGAPLVVPDGRV
                     ITPSRN"
     gene            complement(1218..2669)
                     /locus_tag="PLY56_09225"
     CDS             complement(1218..2669)
                     /locus_tag="PLY56_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21702.1"
                     /translation="MPQTPKLMYNYRGVYHRDLTHEVAYYAGKYTGEYYRMRSGVTTP
                     LCLVANDHRFSSNSIKHYVCQGLIEAGCQVVDNGCLPTSVASYCIRKRADGGVVVTAS
                     HNPPEHNGLKFFDSTGGVLKPHVEEVLIAKVLAQVAKDDTIPHRLITRGYALQGPEDV
                     MELYISDVERTITLQHSLRVAVDCRFGTAYTIMPQLLERLGCEAALLHGQLHPYFIKS
                     NGDYLNPEPKESNIGEIRRFIGSGTYDVGLIFDGDSDRNVIVDDKGTYVTDDTVLLLL
                     ALEYADRAKPVAVTIDTALMVEQVLRKEGFELLVTPVGDPFIAQALEDTDASFGGVPN
                     GHYIFPDFNLYSDGIYSAAVMLRLVAKLKSQGTTLSEFVGTLPPGYILKAKHPFGQSR
                     AAFHAEVAPRVRDLIARHCNQIDDRQTDDCVVTGRSDHCKLLVRYNRWDNNFNVQAES
                     LESAGHAQELQDSLMSLLGSICTNASTKARTNA"
     gene            complement(2878..>4124)
                     /locus_tag="PLY56_09230"
     CDS             complement(2878..>4124)
                     /locus_tag="PLY56_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21703.1"
                     /translation="IGTRNYFGERGPFVANARGQFRIEGVQLSPGDQVVLNLEVTHPG
                     FLPGFATSVAPLTVQIGDGFLVAGERSRDILVQAVETSDVIAVLQRVGSTSVIGHSGG
                     EITAPVPQPSTVTTPPAVITVRVPQGALATAVPVSLTPLTPSALVATTAHLPSTLPTP
                     TMPAVPGRPISVSTDDPIDLAVVTPFSAFDLRVGTSANQSFVFPAGYEPSVLCPLPIS
                     LPAGTRVPVMEFVTEPVAAPNAFWRFVTDGIVGPNGRVIEFKISRPGIYAAVAELRWS
                     LLAVLNDSERQVQDFNPLTQFPQGYLATSINATLTIQGLNDSDHPAELAFIRSALAAI
                     LDDTAHGPIRVVVPPGDRFGIRCTPVKRDIGLRLIVGSGEREFTYNGLWRFWRQQQRE
                     LPPLQQIPPHLQGHVQGGGVER"
BASE COUNT          759 a         1248 c         1342 g          775 t
ORIGIN      
        1 caccctgcgt tgattgccag ggtgatgcat accctctgac gccgagcgag gcaccagttg
       61 tgctctaaac atgcgcaggg gcctgatact catggtgcag cgagtatcag gcccctgcgt
      121 acgtctaaac tgcttgtcgc gtgtctaaat tctacgtgcc gctaattgcg tgacggcgtg
      181 atcaccctgc catctggcac taccagggga gcgccatcac cgagcgcgcg cgtgccatcc
      241 ggaaccacga ccccttgtcc gaggatggcg ttatgcacta cgcaatccga gccgaccaga
      301 acgttctcca tgagcatcga gttgccgatg tagctgttgc tgccgatccg gcagccgcta
      361 ccgatgtagc tgcaaccgcc gaggctcacg ttctcatcca ggtgcacatt ggcccccaag
      421 agtacctcgg gggcggcgcc gcggcgcaac gcatggcggt aaccgtctgc catgtcccta
      481 accacgtagg ccaacgcctg catcatatcc gccggggtat tgatgttgac gcgccaccct
      541 tcggcgagac agtagtaaac cggctggcca tcgttgatca gctcttgaat ggcatgtggc
      601 aggtagtact cgccgttggc gacaggctgg atcttctcaa tcgcagggaa gatgcttggg
      661 gtgaaggcat acatccccgc gcaggtgtac ggtcctctgg gtatctcagg ccgctcctcg
      721 atcaggcaga cgcggtcgcc gtccacgcag accacggcgt gcttgcgatg atcaccgcct
      781 gcgcacacgg caaggacgct tccgcctgag tacttctcca ggcttgccgc taggtctcgg
      841 aggcgatagc cggtcatgtt atccccgaag acgaggatga agggcgaatc accaatggcg
      901 tctctcgcct ggtaaacggc gtgggccgtg ccccgaagtt cgtgctgtgc tacataggtg
      961 aggcgcacgc cgattcgcga accatccccg aagtagcgct ccacctcatc ggaatcggga
     1021 ttgaccacga tgacgatgtc atcaatcccc gcgtcgcgca ggttctcaat attgtactgg
     1081 agaatgggcc ggcccgcgat cggcaccaga ggcttaggcc ggcggtcggt gagcgggcgc
     1141 aggcgtgtgc cccgtccggc tgcaagaatc aaccctttaa tcacagagct tccctttccg
     1201 cgtcacacgg ctccgtatca cgcgttggtg cgagccttgg tgctggcatt cgtgcagata
     1261 cttccaagca ggctcatgag agaatcttga agttcctgtg catggccggc cgactccagt
     1321 gactctgcct gcacgttgaa gttgttgtcc cagcggttgt accgtacgag cagcttgcaa
     1381 tgatccgagc gccctgtcac cacgcaatca tccgtctggc gatcatcgat ctgattgcaa
     1441 tgccgggcga tgagatcgcg cactcgggga gcaacctccg cgtggaaagc ggcgcggctc
     1501 tgtccgaagg ggtgctttgc cttgaggatg tagccagggg gcagagtacc cacaaactcc
     1561 gagagggtgg tgccctgact cttcagcttg gccaccaggc ggagcatcac cgccgcggag
     1621 tagatgccat cgctgtagag gttgaaatcc gggaagatgt agtgtccgtt gggcacgcca
     1681 ccgaaggatg cgtcggtatc ctccagcgcc tgcgcgataa aggggtctcc gactggggtt
     1741 acgagcagct cgaatccctc cttgcgcagg acttgctcca ccatgagcgc cgtgtcgatg
     1801 gtgaccgcga ccggcttcgc ccggtcggca tactccaggg cgagcaggag gagaacggta
     1861 tcatcggtga cataggtgcc tttatcatcg acgatcacgt ttcggtcgga gtccccatcg
     1921 aagatcaagc ccacgtcata ggtgccggat ccgatgaagc gccgtatctc gccgatgttg
     1981 ctctccttcg gctcggggtt gaggtaatcg ccgttcgact tgatgaagta gggatggagc
     2041 tgaccgtgga ggagcgcagc ctcgcatccc aggcgctcga gaagctgggg catgatggtg
     2101 taggcggtgc cgaaacggca atcgacagct acgcgcagcg aatgctgtag cgtgatggtg
     2161 cgctcgacgt cgctgatata gagctccatc acatcctctg ggccctggag ggcgtagccg
     2221 cgcgtgatca gccggtgcgg gatggtatca tccttagcga cttgcgcgag cactttggcg
     2281 atcagcacct cctccacgtg tggcttgagc actccccccg tggagtcaaa gaacttcagg
     2341 ccgttatgct ctggcgggtt atgcgatgcg gtcacgacca cgcccccatc ggcgcgcttg
     2401 cgaatgcagt atgaagcgac agatgtcgga aggcacccgt tgtctaccac ctggcacccg
     2461 gcctcaatca gcccctggca aacatagtgc ttgatcgagt tgctggagaa ccggtggtcg
     2521 ttcgcgacga ggcagagcgg ggtggtgacg ccgctacgca tacgatagta ctcccccgtg
     2581 tattttcccg cgtagtaggc cacctcgtgc gtcaggtcac ggtgatagac gccacggtag
     2641 ttgtacatca gcttaggtgt ctgcggcatg gtcccctctt tcgagccagt gcgggctggc
     2701 ttgaagtcaa ctgcctctgg tgcgggggcg tgcctagtag gctctggccc caggaattcc
     2761 ggacagaagg cgcatctcat ccacggtccg ctcttcacgg gttccgcggt ggagcccatc
     2821 tgccttgtgt atgggaggga gcccggagag ccaggctccc tcgtgcagga ggagcggtca
     2881 gcgctcgacg ccaccgccct ggacgtgccc ctgtagatgg ggcggtatct gctgcagcgg
     2941 gggaagttct cgctgctgct gcctccagaa gcgccagaga ccgttgtagg tgaactcgcg
     3001 ctcgccactc cccacgatga ggcgcagtcc gatgtcgcgc ttcaccggcg tgcagcggat
     3061 cccaaacctg tcgccaggcg gaacgaccac gcggatcggc ccgtgggctg tatcgtccag
     3121 gatggcggca agcgcgcttc gaatgaatgc cagctccgcc gggtggtcac tgtcgttcag
     3181 accctggatg gtcagcgtcg cgttgatgct ggtggccagg tagccctgcg gaaactgcgt
     3241 cagcggattg aagtcttgca cctggcgctc gctgtcgttg aggacggcga gcagcgacca
     3301 gcgcaactcg gcgaccgcgg catagatccc cggccgggat atcttgaact cgatcacgcg
     3361 cccgttcgga ccgacgatgc catcggtgac aaagcgccaa aaagcattgg gcgcggcgac
     3421 gggctcggtg acgaactcca tgaccggcac gcgcgtgccg gctggcagcg agatcggcaa
     3481 ggggcagagc acagaaggct catagcccgc agggaagacg aagctttggt tggcgctggt
     3541 gcccacgcgg agatcgaacg ccgagaaggg ggtcactacc gcgagatcga tcggatcatc
     3601 ggtgctgacc gagatcgggc ggcccggcac cgcgggcatc gtcggcgtcg ggagagtgga
     3661 cgggaggtga gcagtggtcg caacgagagc gcttggggtg agtggcgtca ggcttaccgg
     3721 cacggcggtg gctagcgcgc cctgcggcac gcgaacggtg atgacggcgg gaggcgtcgt
     3781 gacggtgctc ggctggggta cgggtgctgt gatctccccc cccgagtggc cgatgaccga
     3841 cgtgctgccg acccgctgca ggacggcgat cacatcggag gtttccaccg cctgcaccag
     3901 gatgtcgcgc gagcgctctc ccgcgacgag gaagccatcg ccgatctgga ctgttaacgg
     3961 cgccacgctg gtggcgaagc cggggagaaa gccggggtgg gtgacctcca ggttcagcac
     4021 cacctgatcg ccgggcgaga gttgcacgcc ctcaatccgg aactgcccgc gagcgttcgc
     4081 cacgaagggg ccccgctcgc cgaaatagtt gcgcgtgccg atct
//
LOCUS       DAOSCV010000335         4118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13669_length_4118_cov_2.878415, whole genome shotgun sequence.
ACCESSION   DAOSCV010000335 DAOSCV010000000
VERSION     DAOSCV010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13669_length_4118_cov_2.878415"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PLY56_09235"
     CDS             <1..1190
                     /locus_tag="PLY56_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOJ21704.1"
                     /translation="RDEAEIRGHRRTYIGSMPGRVIQAIRQAGTRNPVFMLDEVDKVG
                     ADFRGDPTAALLEALDPEQNTAFSDHYLEVPFDLSDVMFITTANLLDPIHPALKDRME
                     VIHFPGYTEAEKLEIARRFLVPKQREAHGLSSDHLVITDEALVRLIREYTREAGVRNL
                     EREIAAICRKIARRIASGNTDPIRVTADDVPGYLGPRQYRYGMAEEHDEVAVALGLSY
                     TEFGGDVLPIEVILMRGKGNLMLTGQLGDVMKESAQAAHSWLRARAQQLGIDEEVFGR
                     TDIHVHVPAGAVPKDGPSAGITIATALASAFTGRAVRKEIAMTGEITLRGRVLPVGGV
                     KEKVLAAHRAGVKRIVLPEENDKDLEEIPDNVRGDLEFLFVKSVDEVLAAALVPSAAA
                     GHA"
     gene            1295..3226
                     /locus_tag="PLY56_09240"
     CDS             1295..3226
                     /locus_tag="PLY56_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21705.1"
                     /translation="MILAVLAALVLVFSSTGCRKEPADQELPQAKLVSEDPWLLAIKE
                     FGEARAYLGNGYLGVNASQEGLGFDREEQLPTFISGHYVNESFAPGPAWAAPNLSIDG
                     QRVTAAEARSYEQTFDLRRGVLVTRYVWRGKPRTEVEITLFPARHDPYLGVIQVALTP
                     ARKARVSLDAVTSAEGCAVAAVDRLADGSPITFPGEVELSARERWVLTRYVGICAERD
                     AADPQATARQSAEAAARAGFDAVLSEHTEAWERLWKRDIIIEGDPAAQRVVNALRYYL
                     LASVREGRADSVPPMGLSSSIYQGHIFWDADVWVFPALVPQYPELARSIVEYRYRTLP
                     AAMENARKQGYRGADYAWESAESGKETVSGEFSRGRHVNAGIALAQWQYYLWTGDREW
                     LRTRGFPVLRATADYWVSRAWHNEPAARYEILDVMGPDEVRGPVDNNAWTNSMAQLNL
                     EVAARAAEIVGVPGDPRWRTVAEKLWIPFDATEKRYVQCGAPDQRGHKQADCELLLYP
                     RRLKMAEDVAARTFDHYKATVSRYGPAMTTSIYAIIAAQLGRAEEAGKHFKESYEPFL
                     LPPYYLYSEKRTSPRPCFVTGLAGTLQAILYGFAGMEPTEDGLRTKFLLPPGWERLKI
                     TGIQWRGETRDLDSYRRGP"
     gene            complement(3269..>4118)
                     /locus_tag="PLY56_09245"
     CDS             complement(3269..>4118)
                     /locus_tag="PLY56_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ21706.1"
                     /translation="CREFGFDGVQLSSGVTAEAEAFGARVEQPADGPPILREYLLADL
                     RRLDALRAIDPSQGGRMPLFFEAVARVVSSLGDEGFVLATLRGPLLAAAQLRGVEQIL
                     LDTIDHPREVEAILDFTAETALHLGKQLLATGAHGLILGEATCSPNFISPALYRRLVL
                     PRHRALIGRLKDAGWQAVGIHICGDITPIIEDIISTGADFMDVDYQVTPEAALARACG
                     RIALRGNLDPASLLRFGTPEAIETVTAALRASVTGTQWILSTGCDIPPATPAANVRAF
                     ATAARS"
BASE COUNT          698 a         1344 c         1431 g          645 t
ORIGIN      
        1 tccgggatga ggccgagatc cgcgggcacc ggcgcaccta tatcggctcc atgcccggcc
       61 gggtgatcca ggcgatccgc caggctggca ctcgcaaccc ggtctttatg ctggatgagg
      121 tggataaggt gggtgccgac ttccggggcg acccgacggc ggccctgctg gaggcgctcg
      181 acccggagca gaacacggca ttcagcgacc actatctgga ggtccccttc gatctctcgg
      241 acgtcatgtt catcacgacg gcgaacctgc tcgatccgat ccaccctgcg ctgaaggatc
      301 gcatggaggt gatccacttc cccggctaca ccgaggcgga gaagctggag atcgcccgcc
      361 gcttcctggt gcccaaacag cgcgaggcgc atgggctgtc gtccgaccac ctcgtgatca
      421 ccgatgaggc gctcgttcgc ctgatccgag aatacacccg cgaggcgggc gtgcgcaacc
      481 tggagcgcga gattgccgcc atctgccgaa agattgcccg ccggattgcc tcgggcaaca
      541 cggatcccat ccgcgtcacg gcagacgatg tgcccggcta tctgggcccg cgccagtacc
      601 gctacggcat ggccgaggaa catgacgagg tggcagtcgc cctcggtctc tcctacaccg
      661 agtttggtgg cgatgtgctt cccatcgagg tgatcctcat gcgcgggaag ggcaacctga
      721 tgctcaccgg gcaactgggc gacgtgatga aggagtcggc gcaagcagcc cactcgtggc
      781 tgcgagcgcg cgcccagcaa ctcgggattg acgaggaagt cttcgggcgc accgatattc
      841 acgtccacgt gccagccggt gccgtgccca aggatggccc ctccgccggg atcaccatcg
      901 ccacggcgct cgcttcggcg tttaccgggc gcgcggtgcg caaggagatc gccatgaccg
      961 gcgagatcac cctccggggg cgggtgctgc ccgtcggcgg cgtgaaggag aaggtcctcg
     1021 ccgcgcatcg cgccggggtg aagcggatcg tgcttcccga ggagaacgac aaggacctgg
     1081 aggagatccc ggacaacgtc cggggcgacc tcgagttcct cttcgtcaag agtgtcgatg
     1141 aagtgttggc cgcggccctg gtgcccagcg cggccgcagg ccatgcctaa gaagagccgt
     1201 gacccgagag cagaaaccag agaccacgca acgacatgat accggaaccg ccgggcagcc
     1261 gcgcggaccc cgcgtgcttt ggcgttccgg tgcgatgatc cttgccgtgc tggcggcgct
     1321 ggtgctcgtc ttctcaagca ccgggtgtcg gaaggaaccc gccgatcagg agctgccgca
     1381 ggcgaaactc gtttcggagg acccctggct gctcgccatc aaggagtttg gagaggcgcg
     1441 cgcctatctg gggaatggct acctgggggt gaacgcctcg caggagggcc tgggcttcga
     1501 ccgcgaggag cagctcccga cgttcatctc cgggcattat gtgaatgaga gctttgctcc
     1561 cggtccggcc tgggcggcgc cgaatctgag catcgacggc cagcgcgtca ccgccgccga
     1621 agcgcgctcc tacgagcaga cgttcgacct gcgccggggt gtcctggtga cgcgctatgt
     1681 ctggcgcggc aagccacgga ccgaggtcga gatcacgctt ttccccgcgc ggcatgatcc
     1741 ctatctgggc gtgatccagg tggcgctgac gcctgcccgg aaggctcgcg tctcgctgga
     1801 cgcggtgacc tccgccgaag gctgcgcggt ggccgcggtg gaccgtctgg cggatggctc
     1861 cccgatcact tttccaggcg aggtcgagct ttcggcccgg gagcgctggg tactgacgcg
     1921 gtatgtcggg atctgcgcgg agcgcgacgc ggctgatccg caggccacag ctcgccagag
     1981 cgccgaagcg gccgccaggg cgggcttcga tgccgtcctc agcgagcaca ccgaggcgtg
     2041 ggaacggctg tggaagcgag atatcatcat cgagggcgac ccagctgcac aacgggtggt
     2101 gaacgccctt cgctactacc tcctggcgag tgtgcgcgag ggccgcgccg acagcgtgcc
     2161 tccgatgggc ctctccagca gcatctacca ggggcatatc ttctgggatg ccgatgtctg
     2221 ggttttcccc gccctggtgc cccagtatcc ggagttggcc cgctcgattg tcgaataccg
     2281 ctatcgcacg cttcccgccg cgatggagaa cgcgcggaag cagggttatc gcggcgcgga
     2341 ctatgcctgg gagtcggcgg aaagcggcaa ggagaccgtc tccggcgagt tctcgagggg
     2401 ccgacatgtc aacgccggga tcgccctggc gcagtggcag tactacctct ggacgggcga
     2461 ccgcgaatgg ctgcgcacgc gcgggtttcc ggtgcttcgc gccaccgcgg actactgggt
     2521 gagccgcgcc tggcataacg agccggcggc ccgctatgag atcctggacg tgatggggcc
     2581 cgacgaggtc cgcggccccg tggacaacaa cgcctggacc aactccatgg cgcagctcaa
     2641 cctggaggtc gcagcgcgag cggccgagat tgtcggcgtg ccaggcgatc cacgctggcg
     2701 caccgtggcg gagaagctgt ggatcccgtt cgatgccacc gagaagcgct atgtccagtg
     2761 tggggcgccc gatcaacggg gccacaagca ggcggattgc gagctactcc tctacccacg
     2821 ccggctgaag atggcggagg acgtggccgc gcgcacgttt gaccactaca aggcgaccgt
     2881 gagccgctat ggcccggcga tgaccacctc gatctacgcg atcatcgcgg cccagctcgg
     2941 gcgcgcggaa gaggcgggga agcacttcaa ggagtcgtac gagccgttcc tcctgccacc
     3001 ctactacctc tacagcgaga agcgcacctc gccccgacca tgctttgtca ccggcctggc
     3061 cggcaccctc caggcgattc tctacggctt cgcgggcatg gagccgactg aggatggcct
     3121 gcgcacgaag ttccttctcc cccccggctg ggaacgcctc aagatcacgg ggatccagtg
     3181 gcgtggagag acgcgtgacc tcgacagcta ccggcgcggg ccgtgatcac gcctcggcag
     3241 ggcgagaggg aacgcttcgc gctggcggct atgatcgcgc ggcggtggcg aaggcgcgca
     3301 cgttggcggc cggggttgcc gggggaatgt cgcatccggt ggagagaatc cattgcgtgc
     3361 cggtgacgct ggcacgcagc gccgccgtga ccgtctcaat cgcctccggg gtgccaaagc
     3421 gcaggagcga cgccggatcg aggttgccgc gcagggcgat gcgcccgcag gcgcgcgcca
     3481 gcgccgcttc gggggtgacc tggtaatcca catccatgaa atcggctccc gtggagatga
     3541 tatcctcgat gatgggcgtg atgtcgccac agatgtggat gcctacggcc tgccacccgg
     3601 cgtccttcag ccgcccgatg agcgcgcggt gtcggggcaa cacgagccgg cggtaaagcg
     3661 cgggcgagat gaagttgggc gagcaggttg cctctccgag gatcaggccg tgggcgccgg
     3721 tggccaagag ctgcttcccg aggtgcagcg ccgtctccgc ggtgaaatcg aggatcgcct
     3781 ctacttccct gggatggtct atcgtgtcca atagaatctg ctcgacgccg cggagctgcg
     3841 ctgccgcgag gagcggtccg cggagagtgg ccaggacgaa tccctcgtcg cccagggagg
     3901 acacgacgcg cgccaccgcc tcgaagaaga gcggcatgcg gcctccttga gagggatcga
     3961 tggcgcgcag ggcatccagt cggcgcaggt cggcgaggag atactcgcgc aggatcggcg
     4021 gcccatcggc gggctgctcc acgcgcgcgc cgaacgcctc cgcctcggcg gtcacgccgg
     4081 aggagagctg caccccatcg aatccgaact cccgacaa
//
LOCUS       DAOSCV010000336         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13716_length_4109_cov_3.734090, whole genome shotgun sequence.
ACCESSION   DAOSCV010000336 DAOSCV010000000
VERSION     DAOSCV010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13716_length_4109_cov_3.734090"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLY56_09250"
     CDS             <1..978
                     /locus_tag="PLY56_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21707.1"
                     /translation="MLADEFCQRQRENGTYSGVAFNDARAARTLLAMHALTGDRRYLA
                     SARRWGEEVLRQQREDGGYRMGYGITSVGEECYVADGGEIATGIIRLALDLPARERGR
                     YLKSLEQYIRFREEFRCEGGGIGVGYCKTDYGVRPMVPLKEIRRIYAPEINLYTIGCT
                     LATAAAHATLFGGLEEQQRAAGDARWLMERQKNTIHGAYVESFIWAHALLPDKALRAE
                     IEAFLRERMIQPLCGPDRPWWIWGAGRSALTLDGFIYAANTFAPEPAVQAEAARAIYG
                     LCADSTPWSLHRLRAEPKWNHHDWIYASYAGVSLADALRPGITLPPTGK"
     gene            982..2349
                     /locus_tag="PLY56_09255"
     CDS             982..2349
                     /locus_tag="PLY56_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ21708.1"
                     /translation="MEREYDVVVVGAGAAGIGAAVGAARNGARTLLVESESAPGGDLV
                     TGLPILGACNARGEWVAGGVLRDLLERVDALGGYIGPVCDWRAVHGVCVDPEALRLVL
                     AAALRESGVRLLLSAIAAGVDVSEGTITGVRVVTRSGQHDVRARYLVDATGDASLCAL
                     AGAPVCAGDGQDHFQPLSLVFRVAGVDFEALLNFAEENPGEFLLAESPVMPTDPRECA
                     RRLRERGYPYLALSASGSLLGRRIGDGSLFPCTAFFLTPTSLAKRELCVNATRLADVD
                     ATDPEQRSKAYGSLAAQAEAALHFMKAELPGCREIVLSAVAHRVGVRETRRIHGEKTL
                     GTEAVIHGLDAPDGIARGAHHVDIHGSGTHQVRIPVRDGGSYAIPFGCLIPRGLRNLL
                     AAGRCLSSTREANGSARVMGTCLATGEAAGTAAALAAARGLKDIRQIPVADLRDTLAA
                     QGAIL"
     gene            complement(2428..3102)
                     /locus_tag="PLY56_09260"
     CDS             complement(2428..3102)
                     /locus_tag="PLY56_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ21709.1"
                     /translation="MNVRRGFTLIELLVVIAIVAILAAILFPVFARARENARKANCQS
                     NLKQVATAMLAYVQDYDECFLSGRYPGTCLFGHSHAAPNAINDYYGWANHLQPYVKNT
                     RVFFCPSTQPTTCTSGSEAARITDAYGYNYDGCCGRSQATVQYPSDQMMFMDMQDSFV
                     IPKRPTYTNCMNGMGNGLTRHSDGANVAFVDGHVKWYAANKIRGMIPQTAGTYCTFLN
                     ITITTD"
     gene            3496..>4109
                     /locus_tag="PLY56_09265"
     CDS             3496..>4109
                     /locus_tag="PLY56_09265"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21710.1"
                     /translation="MSDSVEFRSLAAAVSGGERAVWSDGLVAPARASLLAVLHAAAPA
                     RPLLILTPSTERADQLAEDLLALGVASTRVYPLVTPPAADGQGDAAVALDTGALRERL
                     QVLQDLAAEAPSVVVAPIAAALQPVPDPTSLRESSLLLRPGERADLEELSRRLTAMGY
                     ERVSLVEEAGQFATRDGILDLFPLNDTHPARVDLFGDEVESIRC"
BASE COUNT          643 a         1369 c         1380 g          717 t
ORIGIN      
        1 atgctcgcgg acgagttttg ccagcgccag cgggagaacg gcacctacag tggcgtcgcc
       61 ttcaacgatg cccgcgcggc gcgcacgctc ctggcgatgc acgcgctcac cggcgaccgg
      121 cgctacctgg cgtcggcgcg ccgctggggc gaggaggtcc tgcggcagca gcgcgaggat
      181 ggcggttacc gcatgggcta cgggatcacc tccgtaggcg aggagtgcta cgtggccgac
      241 ggcggcgaga tcgctaccgg gatcatccgc ctggcgctcg acctacccgc gcgcgaacga
      301 gggcggtatc tgaagtcgct ggaacagtat atccgcttcc gcgaggagtt ccggtgcgag
      361 ggtggcggca tcggcgtcgg ctactgcaaa actgactacg gcgtgcgtcc catggtgccc
      421 ctcaaggaga tccggcgcat ctacgcgccg gagatcaacc tctacaccat cggatgcacg
      481 cttgcaaccg ccgcggccca cgccacgctt ttcgggggtc tggaggagca gcagcgtgcc
      541 gccggggatg cgcgctggct gatggagcgg cagaagaaca ccattcacgg cgcctatgta
      601 gagagcttca tctgggcgca cgcgctcctg ccggacaagg cgctgcgcgc cgagatcgag
      661 gcgtttctcc gcgagcggat gatccagccg ctgtgcggcc cagaccgccc ctggtggatc
      721 tggggggccg gccgctcggc gctcaccctc gatggcttca tctacgcggc gaacaccttc
      781 gccccggagc cggccgtcca ggcggaagcc gcgcgtgcca tctacggcct ctgcgcggat
      841 agcacgccct ggtccttgca ccggctgcgc gccgaaccaa agtggaacca ccacgactgg
      901 atctacgcga gctacgcggg agtcagcctg gccgatgccc tgcgcccagg gatcacgctg
      961 cccccaactg gaaagtgacc tgtggaacga gagtacgacg tggtggtcgt gggcgccggc
     1021 gcagcgggga tcggcgccgc cgtgggagcc gcccggaatg gcgcgcgcac gctcctggtg
     1081 gagtcggaaa gcgcaccggg cggcgacctg gtgaccggcc tccccattct gggggcgtgt
     1141 aacgcccgtg gggaatgggt ggcaggcggg gtccttcgcg acctcctgga gcgcgtggac
     1201 gcgctgggtg gctatatcgg ccccgtctgt gactggcggg cggtgcatgg cgtgtgcgtg
     1261 gaccccgagg cgctgcggct agtgctcgcc gccgccctcc gcgagagcgg ggtccgcctg
     1321 ctcctctccg cgattgccgc aggcgtggat gtttccgagg gaacgataac gggcgtgcgc
     1381 gtggtgacgc gctccgggca acacgacgtg cgcgcccgct atctggtaga tgccacgggc
     1441 gacgcctcac tctgcgccct tgctggagcg cccgtatgcg cgggcgatgg gcaggaccac
     1501 ttccagccgc tcagcctggt gtttcgcgtg gcaggagtcg acttcgaggc gctcctcaac
     1561 tttgccgagg agaacccggg agagttcctg ctggcagaga gcccggtgat gcccaccgat
     1621 ccgcgggagt gcgcccggcg cctgcgcgag cgtggctacc cctacctggc gctttccgcg
     1681 tcgggatcgc tcttgggccg gcgcattggt gacggctcgc tcttcccctg cacggcgttc
     1741 tttctaacgc ccacctctct cgcgaagcgc gagctgtgcg tgaacgcgac gcgcctggcc
     1801 gacgtggacg ccaccgatcc cgagcagcgt tccaaagcct atggctctct ggccgcccag
     1861 gcggaggcgg cgctgcactt catgaaagct gagctgcccg gatgccggga gatcgtgctc
     1921 tccgcggtgg cccaccgcgt cggggtgcgc gagacgcgcc gcatccacgg cgagaagacg
     1981 ctcggcaccg aggcggtgat ccacggtctg gacgcgcccg acggcatcgc tcgcggggcg
     2041 catcatgtgg atatccacgg gagcggcacc caccaggtgc ggatccccgt tcgcgacggg
     2101 ggttcctacg ccatcccctt tggctgcctg atccctcgcg ggctccggaa tctcctcgcc
     2161 gccgggcggt gcctctcgtc cacgcgagag gccaacggct ccgcgcgcgt gatggggacc
     2221 tgcctggcca cgggcgaagc cgccggcacc gccgcggctc tcgcggcagc gcgaggcctg
     2281 aaggacatcc gccagattcc cgtggcggat cttcgagaca cactcgcggc tcagggagcg
     2341 atcctctgag ccgcgagcga gcttcgtgcc ctcaaagcga acccgcatcc cggcgcgcct
     2401 gcgggccccc gggtgcgaga gcgaagccta gtccgttgta atcgtgatgt tgaggaaggt
     2461 gcagtaagtg cccgcggtct gcgggatcat cccccggatc ttgttcgccg cgtaccactt
     2521 cacgtgacca tcgacgaagg cgacgttggc gccatcgctg tgccgcgtga gcccattgcc
     2581 catcccgttc atgcagtttg tgtacgtggg gcgtttcggg atcacgaagg agtcttgcat
     2641 atccatgaac atcatctggt ccgatggata ctgcaccgtc gcctggctgc gcccgcagca
     2701 gccgtcatag ttgtagccgt aagcgtcggt aatgcgcgct gcttccgagc cgctggtgca
     2761 ggtcgtcggc tgcgtgctag gacagaagaa gacccgggtg ttcttgacat acggctgcag
     2821 gtggttcgcc cacccgtagt agtcgttgat tgcgttcgga gccgcgtgcg aatggccgaa
     2881 caggcacgtg cccggatatc ggcccgaaag gaagcactcg tcgtaatcct ggacgtaggc
     2941 gagcatcgcc gtggcaacct gcttcaaatt gctctggcag ttcgccttgc gcgcattctc
     3001 ccgggcccga gcgaaaaccg gaaagaggat cgctgcgagg atggcaacga tggcgataac
     3061 gaccagcagc tcaattaacg tgaaccctcg acggacgttc atggcggact cctctcttgg
     3121 gtgattccgt acgtgattcc gtacgggaga gcacaactct ttatactttt cgctattata
     3181 tcagacgcgt ctgcaagtgt caacagaggt tttcccccgt gttcaaaggc cgccccccgc
     3241 cctacccggt gtgcccgcac atccctatcc gcatccgttc acttcatccg tggtatcgct
     3301 cggcctctcc accggacttc cccaaggcgg cgccgggtgg tataatgagt gcagatagat
     3361 tcataccatc tcatatcgcc gagagtgaga ggattcggcg ttggcaatgc cggagcccgt
     3421 tttgtggtgt acggcccggc ccaaagaagt gaactggcga ttgctagaat gcacctttct
     3481 gatattatac ccgttttgag cgattcggta gagttccgct ccctggccgc cgcggtctcg
     3541 ggaggcgagc gcgccgtgtg gagcgatggc ctcgtagccc cggcacgggc atccctcctc
     3601 gcggtgttgc atgcggcggc gccggcgcgg cccctcctca tcttgacgcc cagcacggag
     3661 cgcgcagatc aactcgcgga ggatctcctg gccctcggcg tagcatccac gcgcgtctac
     3721 ccactggtca ccccgccggc agccgatggc cagggggatg ccgcggtggc tctcgatacc
     3781 ggggcgctcc gcgagcgcct ccaggtgctg caagatctgg cggccgaggc accctccgtg
     3841 gtggtcgcgc caatcgccgc ggccctgcag ccggtgcccg atcccacgag cttgcgcgag
     3901 agcagcctcc tgcttcgccc gggagagcgc gcggatctcg aagagctgtc gcgccggctg
     3961 acggcgatgg gctacgaacg tgtctccctg gtcgaagaag ccgggcagtt tgccacgcgc
     4021 gatgggatcc tggacctctt ccccctgaac gacacgcatc cggcgcgcgt tgacctcttc
     4081 ggcgacgagg tcgaatccat ccgctgttt
//
LOCUS       DAOSCV010000337         4107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13728_length_4107_cov_3.481491, whole genome shotgun sequence.
ACCESSION   DAOSCV010000337 DAOSCV010000000
VERSION     DAOSCV010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13728_length_4107_cov_3.481491"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLY56_09270"
     CDS             <1..559
                     /locus_tag="PLY56_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOJ21711.1"
                     /translation="VASRILSLGEKSQRVGEINEIITDIAGKTHLLALNAAIESAAAG
                     EFGARFAVVAAQVKELADETREATSQVKAVVQEIQSAINATVMATGETTAEVDRGAQL
                     AMRSGEAIDEIVERVQGIHVATQQQLAGSEQVVNAMRDIQEVVRQSAAGSQQAAEAAA
                     RLEEIASQLGAVISRLSHDYEEKKG"
     gene            563..1618
                     /gene="cheB"
                     /locus_tag="PLY56_09275"
     CDS             563..1618
                     /gene="cheB"
                     /locus_tag="PLY56_09275"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOJ21712.1"
                     /translation="MRVLIVDDVAPARRAIRSLLEEDPEITVVGEAENGEEAVALALR
                     LRPDVITMDVLMPVLDGYAATARIMSECPTPIVAVTSFSLEDGTVMARMIEAGAVDVM
                     SKAFSRAPKEWERMRRELVHRVKAAARVSVRGIPEGHASAAQVGCNAAFAASRPPAGR
                     RPRLVVVAASTGGPPALRTLLGGLEASLAAPVLVVQHISKGFTAWMAEWLNGSIGRVV
                     SVAQDGERARSGRVYLAPDDRHLRVLPEEILRLSEAPPVRSLRPSADVLFRSAAQAYG
                     RDVVGVVLTGMGDDGARGARAIKEAGGRVLAQSEESATIFGMPRAVIEAGLADEVLAP
                     NEMARRLLFLCGRGEAS"
     gene            1615..3024
                     /locus_tag="PLY56_09280"
     CDS             1615..3024
                     /locus_tag="PLY56_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOJ21713.1"
                     /translation="MRGARDQRGIPEPVLLRIAELIEEQSGLALDDLQCRALDAAVVE
                     RMAALNMGEPWDYLALLRQVRSGELRVLIERIANGETAFFRNAPHFRALRDVVLPVLL
                     TVARRVVFWSAGCATGEEAYSLAIACLEAGLPAVAPVMVLGTDINRSALAAAERGEYA
                     GRHLANVGPELRERYFEPTPRGWRVNDTVRALVSWAPHNLLNTELPVPPESVDLLFCQ
                     NVTIYFRPETIRRVTERLATVLRPGGHFFPGFSEMPWCRAPGLELVSFGDCFAYRKQL
                     PRSSGSVQDRPMKRRLAGGRALNAGEGSLQGSRRARLPQSVPEVPTAAAAESAHPAGS
                     GSLESARQAASRGDLARALQEVSAALEADPMRPEAWELFGLLQRQAGRRDEAERGFRR
                     QLYLAPESPLPHFHLATLFRERGQTNDARREYARAARILETLSPETQLAGVTADLLLR
                     ACRRFGLAGDTESESEGPS"
     gene            3141..>4107
                     /locus_tag="PLY56_09285"
     CDS             3141..>4107
                     /locus_tag="PLY56_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ21714.1"
                     /translation="MLRIGTAQAVITPPIGTPLAGYFSPRPSQGIHDDLYCRALVLDD
                     GETVLAHVACDLISVRRALVAEVRRLVEEATGIPGERVAVSATHIHTGPYTSRLLKGG
                     TDSSYLAILPRTIAGAVIAAYHSRSEGRLGIGSGHVESIAFNRRYVMRDGGVRTNPGV
                     LNPDIVAPAGPIDPQLGVLKATDPAGNPRALWVNYTVHTDVVGGEFISADYPGYLARA
                     VQAVLGPQVIVPYANGASGDLNHINVKGPGAAPEEQRGQEHARWMGTILAGEALRVFM
                     ETPTTDEVRLDAASETFTIPVREVPADLQAWARERLARAEAKRAAA"
BASE COUNT          636 a         1299 c         1515 g          657 t
ORIGIN      
        1 cgtggcgagc cgcatcctct cgctgggcga gaagagccag cgcgtgggcg agatcaatga
       61 gatcatcacc gatatcgccg ggaagacaca cctgctcgcg ctgaacgccg ccatcgagag
      121 cgcggcggca ggcgagttcg gcgcccggtt tgctgtggtc gctgctcagg tcaaggagct
      181 cgccgacgag acgcgcgagg caaccagcca ggtgaaggcc gtggtccagg agatccagag
      241 cgcgatcaat gccaccgtca tggccaccgg cgagacgacc gcggaggtgg atcgcggcgc
      301 gcagcttgcg atgcgctccg gagaggcgat tgacgagatc gtcgagcgcg tgcaggggat
      361 ccacgtggcc actcagcagc agctggctgg tagcgagcag gtggtcaacg ccatgcgcga
      421 cattcaggag gtcgtccgcc agagtgcggc aggaagccag caggcggccg aggctgctgc
      481 gcgcctggag gagatcgcca gccagctcgg cgcggtgatc tcccgcctct cgcacgacta
      541 tgaggagaag aagggctagc tgatgcgcgt cctcatcgtc gacgacgtgg cgccggcgcg
      601 ccgggcgatc cgcagcctcc tggaggagga cccggagatc accgtcgtcg gcgaggcgga
      661 gaacggggaa gaggcggttg cccttgccct gcgcctgcgt ccggatgtga tcaccatgga
      721 cgtgctgatg ccagtcctcg acggctatgc cgcgacggcg cgcatcatgt ctgagtgccc
      781 gactcccatc gtcgcggtca cctcgttttc tctggaagat ggcacggtga tggcccgcat
      841 gatcgaggcc ggcgcggtcg atgtgatgag caaggcgttc agccgcgccc cgaaggagtg
      901 ggaacggatg cgccgggaac tggtccaccg cgtcaaggca gccgcccggg tctctgtgcg
      961 cgggattccc gaggggcacg cgtccgccgc ccaggtcggg tgcaacgcag cgtttgccgc
     1021 ttcccggccg ccagccgggc ggcggccgcg cctcgtggtc gtggccgcgt cgacgggggg
     1081 gccgccggcg ctccgcacct tgctgggtgg gctcgaagcc agtctcgcgg ctccggtgct
     1141 cgtggtccag cacatctcga agggctttac tgcgtggatg gccgagtggc tgaatgggag
     1201 catcggtcgc gtggtaagcg tggcccagga tggggagagg gcgcgctcag gccgggtcta
     1261 cctggcgccc gatgaccggc atctgcgggt gcttccggag gagatcctcc ggctgagcga
     1321 ggcacctccc gtgcggagcc tgcgcccctc ggcggatgtg ctcttccggt cggctgccca
     1381 ggcctacggg cgggatgtcg tcggcgtcgt gctcacgggg atgggggatg acggcgcgcg
     1441 gggggcacgc gccatcaagg aagcgggcgg ccgggtgctt gctcagagcg aggagagcgc
     1501 gacgatcttc ggcatgcccc gagccgtcat cgaggccgga ttggcggatg aggtgctggc
     1561 gccgaatgag atggcacgtc ggctgttgtt cctgtgcgga cggggagaag cctcgtgagg
     1621 ggagcgcgcg accagagggg catcccggag ccggtgctgc tgcgcatcgc cgagctgatc
     1681 gaggagcaga gtggcctggc gctggatgat ctccagtgtc gcgccctcga tgcggccgtc
     1741 gtggagcgga tggcggcgct gaatatgggc gagccttggg actatctcgc cctccttcgc
     1801 caggtgcgct ccggcgagct acgcgtgctg atcgagcgga ttgccaacgg ggagaccgcc
     1861 ttcttccgca acgcgcccca cttccgtgcg ctgcgcgatg ttgtgctgcc ggttctcctg
     1921 acggtggcgc gtcgcgtcgt cttctggagc gcgggctgtg ccacgggaga ggaggcgtat
     1981 agcctggcga tcgcgtgctt ggaagccggt ctgccggctg ttgcccccgt catggtgctg
     2041 ggcacggata tcaaccgctc ggctctggcg gccgcggagc gcggggagta cgcgggacgc
     2101 cacctcgcta acgtcggccc ggagctgcgc gagcgctact tcgagcctac cccgcgcggg
     2161 tggcgggtga acgacacggt gcgcgcgctt gtctcttggg cgcctcacaa tctgctgaac
     2221 acggagctgc ccgtgccgcc cgaatccgtg gacctcctct tctgccagaa tgtgacgatc
     2281 tacttccggc cagagacgat ccggagagtg acagaacggc tggcaacggt gctgcgccca
     2341 ggcggacact tcttccctgg gttttccgag atgccctggt gccgcgcgcc gggcctggag
     2401 ctagtcagtt ttggcgattg cttcgcctat cgcaagcagc ttccgcgctc ttcaggcagc
     2461 gttcaggatc gccccatgaa gcgccggttg gcaggaggaa gagcgttgaa cgccggcgaa
     2521 ggcagcctcc agggaagccg gcgcgccagg ttgcctcaga gcgtgccgga ggtgccgact
     2581 gctgccgcgg cagagagtgc gcatcccgcc ggatctggaa gcctggagtc cgctcgtcag
     2641 gcagcctcac ggggagacct ggcacgcgcc ctccaggagg tgagcgcggc gctggaagcc
     2701 gatccgatgc gcccggaggc gtgggagctc ttcgggttgt tacagcggca ggcaggacgc
     2761 cgcgacgagg cggagcgtgg attccgacgt cagctctatc tcgcgccaga gtcccccctg
     2821 cctcattttc acctggcgac gctcttccgg gagcgcgggc aaacgaacga tgcgcgccgt
     2881 gagtacgcgc gtgccgctag gatattggag acgctgtcgc cggaaactca gcttgccggc
     2941 gtgacggcgg atctgctgtt gcgcgcctgc cggcggtttg ggctggcggg cgacacggaa
     3001 agcgagagcg agggcccgtc ctgaatcctc cgccgagaca ccctgagagg gcttcccaag
     3061 ggcagaccct ctcgcggatg tcggcgcgaa gaaggcggac gtgccctctg cgagacctcg
     3121 tgaatgacaa ggagagaaag atgctgcgta ttggtacggc tcaggcggtg atcacgcctc
     3181 cgataggaac gccgctggcc ggatacttta gcccccgccc ctcgcagggg attcacgatg
     3241 acctgtactg ccgcgctctg gtcctcgatg atggcgagac ggtccttgcc catgtggcgt
     3301 gtgatctgat ctccgtccgg cgcgctctgg tcgctgaggt gcgtcggctg gtggaggaag
     3361 cgacggggat tccaggcgag cgcgtggcgg tcagcgcgac gcatatccat acagggccct
     3421 acacctcgcg cctccttaaa ggcggaaccg attccagcta tctggccatc ctgccaagga
     3481 caatcgccgg cgcggtcatc gcggcttatc actcgcgctc ggagggccgc ctggggattg
     3541 gcagcggcca cgtggagagc atcgccttca accgccgcta cgtgatgcgt gatggcgggg
     3601 tgcgcaccaa tcccggcgtg ctcaaccccg atatcgtggc gccggccggg cctatcgacc
     3661 cgcagctggg cgttctgaag gcgacagacc cggcagggaa cccccgcgcc ctttgggtga
     3721 actacaccgt ccacaccgac gtggtcggcg gcgagttcat ctccgcggac tatcccggct
     3781 acctggcgcg cgccgtgcag gcggtgctcg ggccgcaggt gatcgtgccg tatgccaacg
     3841 gtgccagcgg cgacctgaac cacatcaacg tgaagggccc gggcgcggcg ccggaggagc
     3901 agcgcgggca ggagcacgcg cgctggatgg ggacgatcct ggcaggcgag gcgttgcgcg
     3961 tcttcatgga gacgccaacc accgacgagg tccggctgga cgcggcttcg gagacgttca
     4021 cgatcccggt gcgcgaggtg cccgcggatc tgcaggcgtg ggcgcgagag cgcctggcgc
     4081 gcgccgaggc gaagcgtgcc gccgcgg
//
LOCUS       DAOSCV010000338         4107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13731_length_4107_cov_2.811945, whole genome shotgun sequence.
ACCESSION   DAOSCV010000338 DAOSCV010000000
VERSION     DAOSCV010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_4107_cov_2.811945"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLY56_09290"
     CDS             complement(<1..1275)
                     /locus_tag="PLY56_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21715.1"
                     /translation="MSDVVIEPKVEGFARTYLLDSITDCLLTAEEPLKVSEIVASIQH
                     DGVFTSRLLRAAMESSDRFQMIDRRWMLAAPEVDPRRPLEANIESVLQHIGRPLASSQ
                     IAQQLAEGLGRPPDVLLSSVKQVLTGRDKYFVVGDRWGLTSWLLDLDDQDEEEILFRN
                     FFLDEDELTRFREKMGSFSWDPGKPIESAARLLNKAGEPVPNKVLQFLAWEVMHRGFR
                     PQEFFAGLFAHEEVYFLSSGHWCGGDVIGEFNQTLEVFTEQLIEAGETAPEEGGEPRE
                     FHVTEEEIAETAAILADRRSHRISEIIEAIYELSPGERDYNAAFGAMWGAMGADERFA
                     WVGGERWRLAGTVPRLTHKIPEILELPYLPYFVNEDGEPVDVELSEDGFEGDLIESVK
                     DPRVMIAGQPVPEGTVPEEAPAKVSVPIRYEHR"
     gene            complement(1676..2632)
                     /locus_tag="PLY56_09295"
     CDS             complement(1676..2632)
                     /locus_tag="PLY56_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ21716.1"
                     /translation="MALFLCLFSLLDGSPAAAAKAASKKPAATFQLPKVRLVAISTPV
                     AMATVRASKKPVSIGTVSYLIGDYRTSYDVLVTPRRDGGLVWMARQQGKSRVQEQGTI
                     GAAQLKNAAEGHLCIIVEKEGDRPSGVTASGKRFRLFSRFSPSYRAMLVACPKQVPTK
                     PVRTALFIESDNQPLGSDEPKGATGAGTAGSPVAWQLGAKAYRAALDAHKRHKKPVAL
                     YFFEKNSKACKQMDSELMTACRVQEVLSPAVKVRIDPSAGAEEKAIAARYGVTSTPAL
                     LIIPAGTDKATWVVPEKPGQKFDPSMPCHFHDLLAAALKPKT"
     gene            3140..4036
                     /gene="modA"
                     /locus_tag="PLY56_09300"
     CDS             3140..4036
                     /gene="modA"
                     /locus_tag="PLY56_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ21717.1"
                     /translation="MRTERNPATAQTSSALRLRSCLRAALGLLAVIALLLAPVAGHAA
                     SGEAPLGEIKRPKGGLVVLCGAGLRAPMEECRKEFERRHKVPIRVIYGGSQCLLAQLA
                     VTYRNALIDIYAPGEEFYAKQAQDRGYVTTYKPMAYFIPVIMVREGNPKKIHTLKDLT
                     KPGLRIGVGEPKACAVGKTTQDLLKKHGLTQQVGKNVVMHTAMAPELGNAVKLGSVDA
                     VINWDAVAAGYEDGAEIIPIPPDQNIIVKSSIGLVKNAREKRMARLFIEFVTGPEGRA
                     IFQKNRYTIDLKAPTYPVKASR"
BASE COUNT          768 a         1329 c         1292 g          718 t
ORIGIN      
        1 gcggtgctcg tagcggatcg gcacggagac ctttgccggc gcctcttccg gcactgttcc
       61 ctcaggaacc ggctggccgg cgatcatcac tcgcgggtcc ttgacgctct cgatcaggtc
      121 accctcgaat ccatcctcgg agagctccac atcgaccggc tcgccatcct cgttcacgaa
      181 ataggggagg tagggcagct ccaggatctc cggaatcttg tgcgtcaacc ggggcaccgt
      241 tccagccagc cgccagcgct cgccgccaac ccaggcaaag cgctcgtccg cgcccatcgc
      301 gccccacatg gcgccaaagg cggcgttgta gtcgcgctcg ccgggcgaaa gctcgtagat
      361 cgcctcgatg atctcgctga tgcggtgcga cctgcggtct gccagaatcg ccgccgtctc
      421 ggcgatctcc tcctcggtga cgtggaactc tctcggctcc ccaccctcct cgggtgcggt
      481 ttcgcccgcc tcgatgagct gctcggtgaa aacttccaga gtctggttga actcgccgat
      541 cacatcgccg ccgcaccaat ggccggatga gaggaagtag acctcctcgt gagcaaagag
      601 accggcaaaa aactcctgcg ggcggaagcc gcgatgcatc acttcccagg cgaggaactg
      661 gagcactttg ttgggcacgg gctcgccggc cttattcagc aatcgggccg ccgattcgat
      721 gggcttccca ggatcccagg agaagctgcc catcttctct cggaagcgcg tcaactcatc
      781 ctcgtccagg aagaagtttc ggaagaggat ctcctcttca tcctggtcat ccaggtccag
      841 cagccaggag gtgagccccc agcgatcccc caccacgaag tacttgtcgc gtccagtgag
      901 cacctgtttc accgaggaga ggagaacatc aggcggacgc cctagcccct cggccagctg
      961 ttgggcaatc tggctcgagg cgagcggccg cccgatgtgc tgaagaacgc tctcgatatt
     1021 ggcttcgagc gggcggcgag gatccacctc cggcgcggcc aacatccaac gccggtcaat
     1081 catctgaaag cggtcgctcg actccatcgc cgcgcgcaag agccgcgacg taaagacgcc
     1141 atcgtgctgg atagacgcga cgatttccga gaccttcagg ggctcctcgg cggtcaacag
     1201 acagtcggtg atactgtcca gcaggtaggt tctggcaaag ccctctacct tgggctctat
     1261 gacaacatcg ctcaagatgc cacctccgca tctgatactc cgggaggagt gtcagggatt
     1321 tcgcatttgc atcgccggcc ctctagcgcg cgccaagcgc gcgaagagca gctgtccgac
     1381 cggcagggcc cgccgggata cggcccttcc ccgcgtccca cgcccgtgca acagagcagc
     1441 gcgatgcacc gcgaaagcag agaacctgag cgcatgaggc agaccggccg cgccggccag
     1501 acaaggtttc tggcggcaac caccgggttc agcgaagcgg ggcgcggccg ttccacgggc
     1561 catatatcca gcctcatgtt accacaatca ggggtgttag tcaatttagg tagacgagcc
     1621 cctcagctcc gtggtccagg gagccggcct ctggaagccg atggagaggg gccgcctacg
     1681 ttttcggttt gagcgccgcc gccaaaaggt catggaaatg gcagggcatc gaggggtcga
     1741 acttctgccc cggcttttcc ggcaccaccc aggtggcctt gtccgtgccg gctgggataa
     1801 tcaggagggc cggcgtcgag gtcacgccat agcgcgcggc aatcgccttc tcctccgcgc
     1861 ccgcgctcgg gtcgatgcgc actttcaccg cggggctcag cacttcttgc acccggcagg
     1921 ctgtcatcaa ctcggagtcc atctgcttgc atgccttcga gttcttctca aagaagtaca
     1981 gcgccacggg cttcttgtgg cgcttatggg catcgagggc agcgcggtag gccttggccc
     2041 ccagttgcca tgccaccggg cttccggcgg tgccggcgcc cgtggcgccc ttcggctcgt
     2101 ccgaacccag gggttggttg tcactctcga tgaacagcgc cgtgcgcacg ggcttggtgg
     2161 gcacctgctt cgggcacgcc acgagcatgg cgcgataact gggcgagaag cggctaaaga
     2221 ggcgaaaacg cttccccgag gcggtcactc ccgatggcct atcgccttcc ttctcgacga
     2281 tgatgcacag gtggccctcc gccgcgtttt tgagttgggc ggcgccaatc gtgccctgct
     2341 cctgcacgcg gctcttgccc tgttgccggg ccatccagac caggccgcca tcgcggcgcg
     2401 gtgtgacgag gacgtcgtag ctggtgcggt aatcaccgat gagatacgag acggtgccga
     2461 tcgataccgg cttttttgaa gcgcgcacgg tggccatggc aaccggcgtg ctgatcgcga
     2521 ccaggcgcac tttcggcaac tggaaagtgg cagccggttt cttgcttgct gccttcgcgg
     2581 ccgctgctgg tgagccatcg agcagcgaaa agaggcagag gaagagagcc acgccggcag
     2641 cggagcgcag cccgggattg cgcgtttata cagccatcat cgtgctaccc cctggctcct
     2701 atcttaccag ctttgcttgg gagcgtcaat caggcgtgcc cgtgagtttc ctacccccgg
     2761 ccccacgcca acggcgatac cacagaggaa ggggatggac gcatgatggg tgggtgcgcc
     2821 cccccggccc gggggaggtt cgaacctgat cacctggtat ctgtgtccat ctgcagatag
     2881 cgaaacgcca gcgcgagatg ttaagcggac actagagatt tgttaagaga tgttaagtcc
     2941 gcccggtttt ctggcgtgca gccgggtgcc cggttgccat cggcgcatga aagatgttat
     3001 gatacttctg caagaaccgc gcgctgtgcg cactatcgtg tcctagagtg ccggtgcctg
     3061 cccctgccta tcggtacaac ttcgtggaag ccgtgtgaga ggccacacgc cggcgccatc
     3121 cggtcgggag gactgagaga tgcggaccga acgcaaccca gcaactgccc agacgagcag
     3181 cgctctccgg ctccgctcgt gcctgcgggc tgccctcggg ctgctcgcgg tgatcgcgct
     3241 tctgctcgcg cccgtggccg gccacgctgc ttcgggcgag gctcccctcg gtgagatcaa
     3301 gcggcctaag ggagggctag tggtcctatg cggcgccggc ctacgcgccc cgatggagga
     3361 gtgccgcaag gagttcgagc gccggcataa ggttcccatt cgcgtcatct atggcggctc
     3421 ccagtgtctc ctcgcgcagc tcgcggtgac ctaccggaac gcgctgatcg acatctacgc
     3481 tccgggcgag gagttctacg cgaagcaggc tcaggatcgc ggctacgtca ccacgtacaa
     3541 gccgatggcc tatttcatcc cggtgatcat ggtgcgcgag ggcaacccaa agaagatcca
     3601 cacgctgaaa gacctgacaa agccgggcct gcgcatcggc gtgggcgagc cgaaggcatg
     3661 cgctgtgggc aagacgacgc aggacctgct gaagaagcac gggctgacac agcaggtggg
     3721 gaagaacgtg gtgatgcaca ctgcgatggc gccggagctg ggcaacgccg tgaagctcgg
     3781 cagcgttgac gccgtgatca actgggatgc cgtggccgcg ggctatgagg atggcgccga
     3841 gatcatcccg atcccgccgg atcagaacat cattgtgaag tcgtccatcg ggctcgtcaa
     3901 gaacgcgcgc gagaagcgca tggctcgcct cttcatcgag ttcgtgacag gacccgaggg
     3961 ccgcgccatc ttccagaaga accgctacac catcgatctg aaggcgccca catacccggt
     4021 gaaagcaagc cgctgacgtg caagccgaag ccacgaaccc agcacgcgcc agcgagcagc
     4081 aagcgcaaaa gccaacccgg agcggga
//
LOCUS       DAOSCV010000339         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13746_length_4103_cov_3.361660, whole genome shotgun sequence.
ACCESSION   DAOSCV010000339 DAOSCV010000000
VERSION     DAOSCV010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13746_length_4103_cov_3.361660"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..2031)
                     /locus_tag="PLY56_09305"
     CDS             complement(334..2031)
                     /locus_tag="PLY56_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21718.1"
                     /translation="MKHEITIIPGVDPREAAKALVNIKDDADLRAVMNRLEPSDAARV
                     IAHAPDIQAQTRLIWSMDKPQRAATLDHLAPVTVAAIVQNQERRNRRLLGDISLEHFA
                     RILEFCSPKQRYYWLALANSFADVGANLLILLLPPKDVAEALLTVPEFRRKRHTLRRF
                     LPGGFDPFPPVEDRRLKAVLVRLAEYDGDRYKEVLETACAMVEEAREQSDTFAELESE
                     PIILPRIPHVPPLMPEEAPVTLDEDEGDQASHPLVPVPLPDASDSLMRAAASKLPPAR
                     RQSLQHEIEKLFRDEVMAQGGSVAMSDLERAAAKLQAYLRLGLRDVPEDPEKVADILT
                     RVRAGDVVQRGITALEQLRQIALRLRPFAAVMDLRQRDLLLALVHPEATIEPKSGEAA
                     LRVPALDRRRRPEAIPVANMHEHLADISVWVAISRALGVRALTEKLASAPNGSLAVLA
                     ALAVSLITRGCWDPETLEMGSLRAFRDEHFDRKQGTWKPGLKETARQKARAWVAQSGI
                     DPSLQPRAVLLIMAALDQLEEFLRTRRVITWERFAPGKRASRRAGGRGANTGGNPKA"
     gene            2220..>4103
                     /locus_tag="PLY56_09310"
     CDS             2220..>4103
                     /locus_tag="PLY56_09310"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ21719.1"
                     /translation="MLRAAFALLLLLLASHVARADVTAVRAGDALVLSNSLIRVRLDL
                     SKGGCPTEVALATEKTSLCGTASLSLYYEGEKRWVTDSSIAGATAKIERRPENAGGGI
                     VVQVDLPAFPGWDLRKSYTLREGSPILSVAYRMRVREALRPYPFIPVNLVGSASADTL
                     LAEGGAVSRDELPVSDFALVMNESPWYAFAKGADGPGVAVALSKWPEWYRRHTIGRRK
                     DGTLFLHARGFPEAFTPGQEVEFSYHLVAFEKEGRRAVAPARALGVAAVPPFEGGAAA
                     PGAASETLARLTQGVRTLCVPQVSTAPVLDGVLDDPCWTVAAVADQFVRSNGSALAAT
                     ETVGRVVYDADALYIGIRCAEPLMANLVTNSREPGPNVWQDDCVEVRLDPRRDRKTSL
                     QFIVNALGVRQDNLLGEGGLTRTRWSAGAHRGADFWSVEIRIPWADLDVTPPRPGDAW
                     GFNLNRERRPVRETTGWCATPAGFHQAERYGDLLFGRGEVEVTRVEPGIDRDGKGIRV
                     YLRSRRAEAVTVTASVAIARDGKTPVFARGEVSVPPKGEATLTLPFEAREAGTYRVAV
                     TLAALGEIFYAVPFTGTLWAEGLNSVLWPAEEHDQTLYLARETLQHFFFIPTKHSQKA
                     YTAF"
BASE COUNT          644 a         1319 c         1422 g          718 t
ORIGIN      
        1 acggcgaccg ggcgcaccga gatcgacgcg agcggcaagt tgtatggcga cctgaagacg
       61 aagtcgctcg tgatcgacga tggcgccgtc ttccagggtg cgtgcatcac cgaggagacc
      121 acgcgggttt cggcgccgaa gcaggatgtc gcctgacgcc gcagcgagag ggtgggggac
      181 tggccggaga aaatcaccgg tacgcaggtt taggcttttg tagaggtggg tattctctga
      241 aagacaagat agcctcccgg tttgcggggt tccgggatgc gattgggcgg ggcgacctcc
      301 agtggccgcc ccgctggttt tttggggcgt tactcaggcc ttgggattgc ccccagtgtt
      361 ggcgcccctt ccgccggcac gccgggaagc acgcttccca ggggcaaacc gttcccaggt
      421 gatgactcga cgcgtccgca ggaactcctc cagctggtcc aacgcggcca tgatcaggag
      481 cacggcccgc ggctggaggc tgggatcgat tcccgactga gccacccagg ctcgggcttt
      541 ctggcgcgcc gtctccttca aacctggctt ccaggttccc tgcttgcgat cgaagtgctc
      601 gtcgcggaat gcccggaggc tgcccatctc gagggtctcc ggatcccagc atccgcgggt
      661 gatcagcgag acggcgaggg cagcaagcac cgcgagcgag ccgtttgggg cgcttgccag
      721 cttctccgta agcgcgcgca cgccaagagc ccgcgagatg gccacccaca ccgagatgtc
      781 ggcgagatgc tcgtgcatat tcgccacggg gattgcttcg ggccggcgcc ggcggtcgag
      841 ggcgggcacg cgcagggccg cctcgccaga cttgggctca atggtcgcct ctggatgcac
      901 gagcgcgagg agcaggtcac gctggcgcag atccatcacc gcggcaaagg ggcgcaggcg
      961 cagggcgatc tggcggagct gctccagcgc ggtgatgcca cgctgcacca cgtctcccgc
     1021 gcgtacccgc gtcaggatgt cggcgacttt ctcgggatcc tccggcacgt cacgcaatcc
     1081 caggcgcagg taggcttgca gcttcgcagc ggcccgctcc aagtccgaca tggcaaccga
     1141 tcctccctgg gccatgacct cgtcgcggaa cagcttttcg atctcatgtt gaaggctttg
     1201 ccgacgggcc gggggaagct tgctcgcggc ggcgcgcatc agcgagtcgg atgcatccgg
     1261 aagcggcacc ggcaccagcg ggtgtgatgc ctggtcgccc tcgtcttcat ccaacgtcac
     1321 gggagcctct tcgggcatga gcggcggcac gtgtgggatc cggggaagga tgatcggctc
     1381 ggactccagc tcggcgaagg tgtcggactg ctcgcgtgcc tcttccacca tcgcgcaggc
     1441 ggtctcgagc acctccttat agcggtcgcc atcatactcc gccagccgga ccagcaccgc
     1501 cttcagacgc cggtcctcga ccggagggaa gggatcgaaa ccaccgggaa gaaaccgccg
     1561 cagcgtgtgg cgtttgcgcc ggaactccgg cacggtgagc agcgcctcgg ccacatcctt
     1621 tgggggcagc agcaggatga gcagattcgc accgacatcc gcgaaagagt tggcgagcgc
     1681 cagccaatag tagcgctgct tggggctgca aaactccagg atccgcgcga agtgctccag
     1741 ggagatatcg cccagaagcc ggcggtttcg ccgctcctgg ttttggacta tggcggcgac
     1801 ggtgaccggg gccaggtggt cgagcgtggc ggcgcgctgc ggtttgtcca tgctccagat
     1861 cagccgcgtc tgtgcctgga tgtcgggcgc atgggcaatg acgcgcgctg catcgcttgg
     1921 ctccagccgg ttcatgaccg cgcgaaggtc cgcgtcatct ttgatattga cgagcgcctt
     1981 cgcggcctct cggggatcca ctcccgggat gattgtgatc tcatgcttca cggtttcttc
     2041 catcatcctc cgtaactcga gtctaccatc gtgggggcgt ggtgtcaaat cggggttcac
     2101 ccggcgtggg ccgggcgggg ccaggagact cgcgccccgg gggtgaattg aggatgggcc
     2161 agggccacca tgcgcatgag aacggctggc ccgcgtggat gcatgtgttg gaggaaaaga
     2221 tgcttcgcgc cgcgtttgcc cttctcttgt tgctgttggc cagccatgtc gcgcgcgccg
     2281 atgtgacggc ggtgcgcgcg ggagatgctc tcgtgctgag caactcgctc attcgggtgc
     2341 gcctggatct ttctaagggc ggctgcccga cggaggtcgc tctggccact gagaagacga
     2401 gcctctgcgg cacggcctcg ctcagcctct actacgaagg ggagaagcga tgggtgaccg
     2461 atagctccat cgctggggcg acggcgaaga tagagcgccg gcccgagaac gccggaggag
     2521 ggatcgtcgt ccaggtcgat ctccccgcct ttcccgggtg ggatctgcgc aagagctaca
     2581 ccctgcgcga gggaagcccg atcctctctg tcgcctatcg catgcgcgtt cgtgaggcgc
     2641 tccggcccta tcccttcatc cccgtgaacc tcgtggggag cgccagcgcg gacacgctgc
     2701 tcgcggaggg cggggccgtc tcacgtgacg agctccccgt gagtgacttt gccctggtaa
     2761 tgaacgaatc gccctggtac gccttcgcga agggcgcgga tgggcccgga gtggcggtgg
     2821 ccctgtcgaa gtggccggaa tggtaccggc ggcacaccat cgggcggcgc aaggacggca
     2881 cgcttttctt gcacgcgcgc ggcttcccgg aggcgttcac gcccggccag gaggtggagt
     2941 tctcctacca cctcgtcgcg tttgagaagg aggggcggcg cgcggttgcg cccgcgcgag
     3001 ccctgggggt ggccgcggtg ccccccttcg agggaggtgc ggccgctccc ggcgctgctt
     3061 cagagacgct cgcgcgcctc acgcagggtg tgcgcacgct ctgcgtccct caggtgagca
     3121 cggcgccggt cctcgatgga gttctcgacg atccctgctg gacggtggcc gccgtggccg
     3181 accagttcgt gcgctcgaac ggcagcgccc tggctgcgac ggagacggtg ggccgcgtag
     3241 tctacgatgc cgacgcgctc tatatcggca ttcggtgtgc cgagcccctg atggccaacc
     3301 tggtgaccaa ctcgcgcgag ccaggcccaa acgtctggca ggatgactgc gtcgaagtcc
     3361 ggctcgatcc ccggcgcgac cgcaagacga gcttgcagtt cattgtgaac gcgcttggcg
     3421 tgcgccagga taatctcctt ggcgagggtg ggttgacgcg gacccgctgg agcgccggcg
     3481 cccatcgcgg cgccgacttc tggagtgtgg agatccgcat cccgtgggcc gatctcgacg
     3541 taaccccgcc tcgccccggg gatgcctggg gcttcaacct caaccgcgag cgccgtccgg
     3601 tgcgcgagac taccgggtgg tgcgccacgc ccgccgggtt tcaccaggcc gagcggtatg
     3661 gcgacctcct tttcgggcgc ggtgaggtgg aagtgacgcg tgtggagccg gggatcgatc
     3721 gcgatgggaa ggggatccgc gtctatctgc gcagcaggcg cgccgaggca gtgaccgtca
     3781 ctgcgtcggt ggctatcgcc cgtgatggaa agacccccgt ttttgcccgc ggtgaggtaa
     3841 gcgtgccccc gaagggcgag gcgaccctca ctcttccctt cgaggcgcgc gaggcaggga
     3901 cctatcgggt ggccgtgacg ctggccgcct tgggcgagat cttctacgct gtgcccttca
     3961 ccggcaccct ctgggcggaa ggtctcaaca gcgtcctctg gcccgccgag gagcacgatc
     4021 agaccctcta cctggcgcgc gagacgctcc aacacttctt cttcattccg accaaacact
     4081 cgcagaaggc gtacactgcc ttc
//
LOCUS       DAOSCV010000340         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13750_length_4102_cov_3.497653, whole genome shotgun sequence.
ACCESSION   DAOSCV010000340 DAOSCV010000000
VERSION     DAOSCV010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13750_length_4102_cov_3.497653"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /gene="lpdA"
                     /locus_tag="PLY56_09315"
     CDS             <1..1142
                     /gene="lpdA"
                     /locus_tag="PLY56_09315"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOJ21720.1"
                     /translation="AVERSRRVTSRLVKGVQGLMKKNGIDVHAGNGYLSGPGRVQVRP
                     DGPELQARNVIIATGGRSRAIPGVAFDGERILSSREAVVLKELPSSMLIIGGGPIGLE
                     FGYVFASYGCAVTVVEMMPHVLPLEDPEVAAVVEKQLTKQGMRIFTGTRVLSVETGDK
                     GVKARVSGPKGEEELEADRILVAIGVQGNIEDLGLEALGIQTERGFIQVNDRMETNVP
                     GIYAIGDVTGKMLLAHVASAQGKIAAEVIAGQSPRPLVYTDMPRAVYCHPQVASVGLT
                     EAMAVERGYEVKTGSFPFRANGKALGQHDWEGFCKFVMDARSGELLGGHLVGPEVTEL
                     LPELGVTRTLEGTVEDIAHTVHAHPTLSEVLHETALVTLGRGVHI"
     gene            1167..2210
                     /locus_tag="PLY56_09320"
     CDS             1167..2210
                     /locus_tag="PLY56_09320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ21721.1"
                     /translation="MNTTPTAPPPSRRLVVMEEDAGTRIDQALASRYDDLTRSRIQKL
                     IESGHITVNGAPTRASYPLRVGDRVCIAFPETTRPEGLLPEEIELDIVYEDEDLLVIN
                     KPVGMVVHPAVGSTRGTLVHALLAYPGALSSIAGEDRPGIVHRLDKDTSGLIMVARND
                     YAHNDLSRQIRERTAGRRYLALVWGNPRFDRVRVEVPIGRHPTDRKKMTVYPAGERRP
                     PSARDATTDLTVLERFTLPCALLEAKLKTGRTHQIRVHCSYIGHPVMGDPQYGGQRKL
                     PVRDLELEDLIERVEGQALHAYRLSFTHPRTGAPMEFTAPPPRPYLELLNYLRAHYPM
                     EGSGPSPLVGLNW"
     gene            complement(2307..3353)
                     /locus_tag="PLY56_09325"
     CDS             complement(2307..3353)
                     /locus_tag="PLY56_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOJ21722.1"
                     /translation="MSSRILCLAVLLAMATPALRADEIQIGGKDRLSGTILGMRGNQV
                     RIGTEHLGEVALPWAAVTDLRSDVDLAIYPEGAEVIVGVITRLAGGVLEIRTAQGIRT
                     LDKSRVNLISTELPRGGKPAPVVKWRGKAEFGFGLVTGNKESNNYLIGTELRRRSPSA
                     EILITAQAEQGQLRQNGAWERDVSYARGSVGLTRNQGGRLYLAGNTTLEHDEISRLAL
                     RQSHAFSVGYRLIPGSPNLWHVELGGNYLWEKYNPLQGDDYSNDGMTLRLRSLTEWKV
                     FGSSRLKLDVVAHPSLEAPHGLRATSNLSLAFPLTGALNLQLSATHDYNTNPRPAGVK
                     ASDLRTRTTLVYGF"
     gene            3649..>4102
                     /locus_tag="PLY56_09330"
     CDS             3649..>4102
                     /locus_tag="PLY56_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ21723.1"
                     /translation="MLTAVVGLLVLSSTARAAEMPWRTSVEDALAEAAKSDKLVMIDF
                     SDTDCVWCDRMENETLADTSVVTACERVIPVRVYVEETPDLARKHEIEGVPSFIFVES
                     TGDEAGRIVGFLPPEEFIHRLNLVLEEQERVVSLRKRVAAEPQDMEAKA"
BASE COUNT          716 a         1253 c         1426 g          707 t
ORIGIN      
        1 cagcggtgga gcgcagccgg cgcgtgacaa gccgcctggt caagggcgtg cagggcctga
       61 tgaaaaagaa cgggatcgac gtccacgcgg ggaacggcta tctctccggg cctggccggg
      121 tgcaggtgcg cccggatggc ccggagctgc aggcacgcaa cgtgattatc gcgacagggg
      181 gccggtcgcg cgctatcccc ggagtcgcgt ttgatggcga gaggatcctc tcctcgcgcg
      241 aggcggtggt gctcaaggaa ctgccctcct cgatgctcat catcggcggt ggaccgattg
      301 gcctggagtt tggctatgtc ttcgcctcct acgggtgcgc cgtgacggtg gtcgagatga
      361 tgccgcacgt gctcccgcta gaggatccgg aggtggccgc cgtcgtcgaa aagcagctga
      421 cgaagcaggg gatgcgcatt ttcaccggca cgcgcgttct ttcggtcgag acgggggata
      481 aaggagtgaa ggcccgcgtt tccgggccaa agggcgagga agagcttgag gcagaccgca
      541 tcctcgtcgc gatcggggtt cagggaaaca ttgaggatct ggggctcgag gcgttgggga
      601 tccagacgga gcggggattc atccaggtga acgaccggat ggagacgaac gtgcccggca
      661 tctacgccat tggcgacgtc accgggaaga tgctcctggc gcatgtggcg tccgcgcagg
      721 ggaagattgc cgcggaggtg atcgccggac agtcgccccg cccactcgtg tacaccgata
      781 tgccgcgcgc cgtttactgc catccccagg tcgccagcgt gggcctcacc gaagcgatgg
      841 ctgtcgagcg cggctatgag gtgaagaccg gctcattccc gttccgggcg aatggcaagg
      901 cgcttggcca gcacgactgg gagggcttct gcaagttcgt gatggacgca cggagcggcg
      961 agttgctggg cggccatctg gttggcccgg aagtgacgga gctgctgcca gagctgggag
     1021 tgacgcgcac gctggagggc acggtggagg atattgcgca cacggtccac gcgcatccga
     1081 cgctttccga ggtgctgcat gagacggcgc tggtgacgct gggccggggg gtgcatatct
     1141 aaggtgatgg ggggaggcgg ggcatcatga acaccacacc tacggcgcct cctccgtcac
     1201 gcaggctggt cgtgatggag gaggatgcag gaacgcgcat cgaccaggcg ctcgccagcc
     1261 gctacgatga cctcacccgg tcgcggattc agaagctcat cgagagcggc catattacgg
     1321 tgaacggcgc gccgacgcgc gccagctacc ccttgcgtgt gggagaccgc gtttgcatcg
     1381 ccttcccgga gacgacgcgc ccggagggcc tgctcccgga ggagatcgag cttgatatcg
     1441 tctatgaaga cgaggatctc ctggtgatca acaagcctgt cggcatggtc gtccatcccg
     1501 ccgtcggttc gacgcgtggc acgctggtgc atgcgttgct cgcctatccg ggcgcgctct
     1561 ccagcatcgc cggcgaagac cggcccggca tcgtccaccg gctcgataag gacacctccg
     1621 ggctgatcat ggtcgcgcgc aacgactacg cgcacaacga tctctctcgc cagatccgag
     1681 agcgcacggc aggccgccgc tatctggcgc tggtgtgggg caacccgcgt ttcgaccgcg
     1741 tgcgcgttga ggttcctatc ggccgacatc cgacggatcg gaagaagatg accgtctatc
     1801 ccgccggcga gcgccgccct ccgagcgccc gtgacgccac caccgacctg acggtgcttg
     1861 agcggttcac cctaccgtgc gcgctcctgg aagcgaagct gaaaaccggt cgcacccacc
     1921 agatccgcgt gcattgctcg tacatcggcc accccgtgat gggcgacccg cagtatggcg
     1981 gccaacgcaa gctgccggtg agggatctgg agttggagga cctcatcgag cgggtggaag
     2041 gccaggcgct tcatgcctac cggctcagct tcactcaccc gcgcaccggg gcgccgatgg
     2101 agttcacggc accgccgccg cgcccctatc tggagctgct caactatctc cgcgcgcatt
     2161 acccgatgga aggaagcggg ccgtcgcccc tggtggggct aaactggtag ccagtttgcc
     2221 ctctcgaggg ggcggctgca gaatacgaga agggcatggc cggaggcaac ctgccatgcc
     2281 cttctgctat ctatcagacg ttctgctcag aagccgtaga ccagggtcgt acgggtccgc
     2341 aggtcagagg ccttgacgcc ggccgggcgc gggttcgtgt tgtagtcgtg agtcgcgctc
     2401 agctggaggt tgagagcccc ggtgagcggg aaggcgagcg acaggttgct ggtcgcgcga
     2461 agcccgtggg gggcctccag gctgggatgg gcaacgacat ccagcttgag gcgcgaggag
     2521 ccaaacacct tccactccgt gagcgagcgc aggcggaggg tcatgccgtc gtttgagtag
     2581 tcatcgccct ggagcgggtt gtacttctcc cagaggtagt tgccgccgag ctccacatgc
     2641 cagaggttag gcgagcccgg gatcaggcga tagccaacag agaaggcgtg gctctggcgc
     2701 agcgccagcc ggctgatctc atcatgctcc agggtagtgt tgcccgccag gtagaggcgc
     2761 ccgccctggt tgcgggtaag gccaacgctg ccgcgcgcgt aggagacgtc gcgttcccag
     2821 gcgccattct ggcgaagctg gccctgctcg gcctgcgcgg tgatcaggat ctcggccgaa
     2881 gggctgcgcc ggcgtagctc cgtgccgatc aggtagttgt tgctctcctt gtttccagtc
     2941 accagcccaa agccaaactc tgccttgccc ctccacttga cgaccggggc gggcttccca
     3001 cctcggggga gctcggtgct gatcaggttg acccggctct tgtcgagggt gcggatgccc
     3061 tgcgccgtcc tgatctcaag gacgccgcca gcgagccgtg tgatgacgcc gacgatcacc
     3121 tctgctccct cggggtagat agcaaggtcc acatcactgc gcaaatccgt cacggcggcc
     3181 cacggaagcg ccacctcgcc gagatgctcg gtgccgatgc gcacctggtt ccctctcatc
     3241 ccaagaatgg tgccgctcag gcggtccttc ccaccgatct gaatctcatc tgcgcgcaga
     3301 gccggggttg ccatcgccag gagcacggcg aggcacagaa tacgtgagga catgacgctg
     3361 ctcctttctc aatcacggtg catggtgctg aaccgaggcg tggaggacaa gtggactcca
     3421 agaccggggc tactataaca tgcgctcact ctgctgtcaa gcgcgcgagg cgtgtggccg
     3481 cggcgtctcc gcctgcgttg ggggtatgga atcgcgagtg tttcatcttt ctccccagcg
     3541 ggcatcttgc cactatggac ggacccatgc cgtaacgctc aagagagaat gccgctcgca
     3601 cgatttgggg gcggggggaa aaggaatatg ctgcggagag gttggatagt gctgacagcg
     3661 gtggtgggcc tgctggtcct ctcttccacc gcgcgggcag cggagatgcc atggcgcacc
     3721 agcgtggagg acgccctggc cgaggccgcc aagagcgata agctcgtcat gatcgacttc
     3781 tcggatacgg actgcgtctg gtgcgaccgc atggagaacg aaacgcttgc cgatacctcg
     3841 gtggtgacgg cgtgcgagcg cgtgatcccg gtgcgggtct atgtggagga gacgccggac
     3901 ctggctcgga agcacgagat cgagggcgtg ccgagcttca tcttcgttga gtccaccgga
     3961 gacgaggcgg ggcgcatcgt cgggttcctg ccgccggagg agttcatcca ccgtctgaat
     4021 ctggtcctcg aagagcagga gcgcgtggtg agcctgcgca agcgggtcgc cgcggagccg
     4081 caggatatgg aggccaaggc cg
//
LOCUS       DAOSCV010000341         4099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13764_length_4099_cov_3.533383, whole genome shotgun sequence.
ACCESSION   DAOSCV010000341 DAOSCV010000000
VERSION     DAOSCV010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13764_length_4099_cov_3.533383"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLY56_09335"
     CDS             complement(<1..819)
                     /locus_tag="PLY56_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOJ21724.1"
                     /translation="MAGERTEAATPKHRQEARKRGQVARSHDLTAGIALTAGFAVLIA
                     SQGFFRQTLESAFHLAYADLHRPDKVFNSIFLWMAPIVRQGAVALAPICAATALAALA
                     TQLAQVGFLVSSQAVRFDLNRINPLEGIKRLVTMRTAVELLKGIVKCLILGGIIAHWT
                     YRQRLAFMELAGLPVSVGVAAFADLCRGLATRVCVALLAIGAIDYFYQRFEYNRSLRM
                     TKQQVKEEYREQEGDPQLKARVRQIQREMARRRMVQDVAAADVVITNPTHLAVAL"
     gene            complement(809..1615)
                     /gene="fliR"
                     /locus_tag="PLY56_09340"
     CDS             complement(809..1615)
                     /gene="fliR"
                     /locus_tag="PLY56_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOJ21725.1"
                     /translation="MAVIDYIAAHIVVWLLVLVRVSGLFVTAPIFGNYQVPAQVRILM
                     ALAFTVVLSPLAVPQTAPFPSAVLPFAAAILREALLGLALGFIPMLLFLALQYAAELI
                     DLQVGLGMGGWVDPTFRSTISPLSYLQYLLALVLFLVLDGHHVVLEGLANSFQMIPLG
                     TFSYTSAMTSGFVELFIRMGLIGMQVAAPVLSILLIADLGLGILGRAAPQLNLLIQSL
                     SVKAVVALLALAAALPLFGYLMAHLFAEAREQLALFLRISTSTLGVGYGR"
     gene            complement(1617..1889)
                     /gene="fliQ"
                     /locus_tag="PLY56_09345"
     CDS             complement(1617..1889)
                     /gene="fliQ"
                     /locus_tag="PLY56_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOJ21726.1"
                     /translation="MLSQEAVLELARGALTLAVQLMLPAVGLSMMVGLAISVVQAATQ
                     IQESTLQVVPRIIAVFVALALFGSWMLRMAIDFTIRVFTQMPGLAK"
     gene            complement(1901..2650)
                     /gene="fliP"
                     /locus_tag="PLY56_09350"
     CDS             complement(1901..2650)
                     /gene="fliP"
                     /locus_tag="PLY56_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645606.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOJ21727.1"
                     /translation="MARILSRTLVLLGAGLLLPGIAFAQEIPRVNVSIGGAQGPRDVA
                     ATLQIILLLTVLSLAPAILLTLTSFVRVVIVLAFTRSALGVQQVPPNQVLIGLALFLT
                     FFLMQPVWKEINSQALQPYLAHQITLDEAVRRGEGPVRAFLFSQTRPKDLALFLRLGG
                     VEQPRTRADVPTYVLVPAFLISELKTAFQMGFMIYVPFLVIDIVVAVILLSMGMMMLP
                     PVMISLPFKVLLFVMVDGWNLVIQSLMLSFR"
     gene            complement(2635..3570)
                     /locus_tag="PLY56_09355"
     CDS             complement(2635..3570)
                     /locus_tag="PLY56_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOJ21728.1"
                     /translation="MLRDRSGQAPEGRRRCASLPACIPAAWTQMRDLLAANPALRKRV
                     MIGAAAMGILVLAYIGGAFRPSDGRGQQAASPAANVQAAQQGSSPVQGRLPASPLTGR
                     PEDEIPLDLTEASASPEKGGGLRLVGWLFGVALKLGVVLALAYVCLSLLKRFMPGAAA
                     TDSDADVGIRRTIVLGAGRTIHLLDVEGHRLLIGCTPQQISLLADLTGAVAPEEEVEG
                     ELDTSGQGAESFRAQLGALLEKQEASQPAPERKPVAAPEATPEVRVPGPETYTDEELR
                     EEVRRRLFTLNRSAAGLQRLAERAREGEQERWPGS"
BASE COUNT          828 a         1370 c         1285 g          616 t
ORIGIN      
        1 cagggcgacg gccagatgcg tggggttggt gatcacgaca tcggcggcag cgacatcttg
       61 caccatccgc cggcgtgcca tctcgcgctg aatctgccgc acgcgcgcct tgagctgcgg
      121 atcaccctcc tgctcgcggt actcctcctt cacctgctgc ttggtcatcc gcaggctgcg
      181 gttatactcg aaccgctggt agaagtaatc aatcgcgcca atcgccagaa gagccacgca
      241 gacgcgcgtt gccaggccac ggcagagatc cgcgaaggca gcgacgccca cgcttactgg
      301 caggccggcc agttccatga aggccaggcg ctgccgatac gtccagtggg cgatgatccc
      361 tcccaggata aggcacttga ctatcccctt gagcagctcg acggcggtgc gcatcgtcac
      421 cagccgcttg attccctcca gtggattgat gcggttgaga tcaaaccgca cagcctgcga
      481 gctgacgagg aagccgacct gggccagctg ggtcgccagg gctgcgagag cggtcgccgc
      541 gcagatcggc gcgagggcaa ccgcgccctg gcgcacgatg ggcgccatcc ataggaagat
      601 agagttgaaa accttgtcgg gccggtgaag atcggcatag gccaggtgga acgcgctttc
      661 cagcgtctgc cggaagaagc cctgcgaagc gatcaacacc gcgaagccag ccgtgagagc
      721 gataccagcc gtgaggtcgt ggctgcgcgc cacctgaccg cgtttgcgcg cttcctggcg
      781 atgtttgggt gttgcagcct cagttcgttc accggccata acccaccccc agggttgacg
      841 tcgagatgcg caggaagagc gcgagttgct cgcgcgcttc tgcgaagagg tgcgccatca
      901 gatagccgaa cagcgggagc gcagctgcta gtgccaacag ggcaaccact gccttgaccg
      961 atagtgattg aatcagcagg ttgagctggg gtgccgccct gcccagaatc ccgagtccga
     1021 gatccgcgat gagcaagatc gagagcaccg gggcggccac ctgcatgcca atcagcccca
     1081 tccggatgaa gagctccaca aacccggagg tcatcgcgct ggtgtatgag aaagtcccga
     1141 gcggaatcat ctggaagcta tttgccagcc cctctaggac aacgtgatgg ccatcgagca
     1201 ccaggaacag caccagggcc aggaggtact gcaggtagct cagcggggag atggtggatc
     1261 ggaaagtggg gtcgacccat ccgcccatgc ccaggcccac ctgcaggtcg attagctctg
     1321 cggcgtactg caacgcgagg aagagcagca tcgggatgaa gccaagcgcc aacccaagga
     1381 gcgcctccct caaaatggcc gcggcaaagg gaagcaccgc cgatgggaag ggcgccgtct
     1441 gcggcaccgc cagcggcgag agcaccacgg tgaacgccag cgccatcaag atgcgaacct
     1501 gggccggcac ctggtagttc ccgaagatcg gcgccgtgac gaagagaccg gagacacgca
     1561 ccaacaccag gagccagacc acgatgtgcg ccgcgatgta atcgatgacc gccattctac
     1621 ttcgccaatc ccggcatctg cgtgaagaca cgaatggtga aatcgatcgc catgcgcagc
     1681 atccacgatc cgaagagcgc cagggccacg aagaccgcga tgatgcgcgg cacgacctgc
     1741 agcgtgctct cctgaatctg cgtcgctgcc tgcacgacgc tgatagcgag cccgaccatc
     1801 atcgagagcc caaccgccgg cagcatcaac tgcaccgcca gagtgagcgc gcctcgcgcc
     1861 agttcgagaa cggcttcctg cgacaacatc cgaccgctcc ttaccgaaag ctgagcatca
     1921 acgactggat gaccaggttc cacccatcca ccatcacgaa gagcaacact ttgaagggca
     1981 acgagatcat caccggcgga agcatcatca tgcccatcga caggaggatc acggcgacca
     2041 caatgtcgat caccaggaaa ggcacgtaga tcatgaagcc catctgaaac gccgtcttta
     2101 gctcgctgat gaggaaagcg ggcaccagca cgtaggtagg tacatcagct cgtgttcgcg
     2161 gctgctccac acctcccagg cgcaggaaga gcgctaggtc cttgggccgg gtctgcgaga
     2221 agaggaaagc gcgcactggg ccttcaccac gcctcaccgc ctcatcaagc gtgatctgat
     2281 gcgccaggta aggctgaagc gcctggctgt tgatctcctt ccataccggc tgcatcaaga
     2341 aaaaggtgag aaagagcgcc agtccgatga gcacctgatt cggcggtacc tgctgcacgc
     2401 cgagcgcgct gcgcgtgaaa gccagcacaa tcacgacgcg cacgaaagaa gtgagcgtca
     2461 gcaggatcgc aggcgcaagg gaaaggaccg tgagcagcag gatgatctgg agcgtggcgg
     2521 ccacgtcgcg tgggccctgg gccccaccaa tgctcacgtt cacgcgcgga atctcctggg
     2581 cgaaggcgat gcctggcaga agcaggcccg cgccaaggag caccagagtt ctgctcagga
     2641 tccgggccat cgctcctgct ccccttctct cgcgcgctcg gcgagcctct gtagccccgc
     2701 agcactccgg ttgagcgtga agaggcggcg gcgcacctcc tcgcgaagct cctcatccgt
     2761 gtacgtctcc ggccccggca cccgcacctc cggggtcgcc tccggcgccg ccaccggctt
     2821 tcgctctggc gcgggctggc tcgcctcctg cttttccagg agcgcgccta actgagcccg
     2881 gaagctctcc gctccctggc cactcgtgtc cagttccccc tcgacctcct cctcgggcgc
     2941 caccgccccc gtgaggtcgg ccaggaggga gatttgctgc ggcgtgcagc cgatcagaag
     3001 gcggtgcccc tccacgtcca acaggtggat agtccgcccg gccccgagca cgatggtgcg
     3061 ccggatcccc acatcggcat ccgagtccgt cgctgcggcc cccggcataa accgcttgag
     3121 aagagagagg cagacgtaag cgagcgcgag caccacgccc agcttcaaag ccacgccaaa
     3181 gagccacccg accaggcgca agccgccccc cttctcaggg ctggccgaag cctcggtgag
     3241 gtcaagaggg atctcatcct ctggccggcc cgtcagcggc gaagcgggca gacgcccctg
     3301 caccgggctt gaaccctgtt gcgccgcctg aacgttcgcc gcaggcgaag ccgcctgctg
     3361 gccacgcccg tccgaaggcc ggaaggcgcc accgatgtaa gcgagcacca ggatccccat
     3421 cgccgccgct ccgatcatca cccgtttgcg gagcgcaggg ttcgccgcga gaagatcgcg
     3481 catctgggtc catgcggccg gaatgcacgc cggcaggctg gcacatcgcc gccggccctc
     3541 cggtgcctgc ccgctcctat ctcgaagcaa ggacccactc tcgtcgctct tcactgcttg
     3601 ctctcctctc tcccccgccc gggcggcacg cgcccgggct acagagagac tcagcaagca
     3661 tcttgccacg ttgcccccac ccggtgtcga taccacagat gaaggagacg gacacggatg
     3721 agcagggcgg gcgcgcctag gatggtgcgc ctcacccggg atcgggtcat ggccatccgc
     3781 gtggggcatc cgaagggccg cgaagcgatc aggcgatttc ctggtcccga gcacgccgca
     3841 ggggtgtgga atctttcctc agcaagctgc gcgtgtgggg cgaacggccg catatggagg
     3901 gggaacgata ggctggcatc ctccgggggg tgagtgccat gcctcggctc atcctggagg
     3961 gcatagcgaa cgcgcagcgt gccgctggaa tccccgcctc tctctccggg cgcgtggaga
     4021 gagaggcggc agagtgaaac cggctggcgc cggcacttca aggcagcacg cgccatgcga
     4081 gtgggaactc tatcacgcg
//
LOCUS       DAOSCV010000342         4087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13823_length_4087_cov_3.604415, whole genome shotgun sequence.
ACCESSION   DAOSCV010000342 DAOSCV010000000
VERSION     DAOSCV010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13823_length_4087_cov_3.604415"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="PLY56_09360"
     CDS             complement(<1..493)
                     /locus_tag="PLY56_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21729.1"
                     /translation="MMRYRLLPLLIGMALSGLAAAGSADPAASANAMEKMLLFGGDTA
                     KEWSAAECTVEASRDRVKATLTALRWSIPVDHTTGEPNYPVGWPRFGRTIPEGPLRDW
                     RGWDYLHFWVYTETSRDTLPREPASLGLHVPDRARRWSRSLAELKKGEWVEVKIPLSQ
                     IPDH"
     gene            complement(615..1163)
                     /locus_tag="PLY56_09365"
     CDS             complement(615..1163)
                     /locus_tag="PLY56_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin"
                     /protein_id="HOJ21730.1"
                     /translation="MKEPKGFIGLTRFGPVAAEIEEHLRCELARRFGLPIRRLGEELA
                     PVGWNPERQQYLGEAMLAELHHAAPPDAVRVLALTEVDLYAPEMEFIFGQAEVGGRTA
                     IVSLHRLHPEASGEAPNRILFLRRALVECVHELGHTFGLVHCPFASCVMHFSEGLEET
                     DRKGPGFCHEHARQLRAALAQV"
     gene            1588..>4087
                     /locus_tag="PLY56_09370"
     CDS             1588..>4087
                     /locus_tag="PLY56_09370"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOJ21731.1"
                     /translation="MIPVLPLSNRVTSFGSEGIALIHLSWRNTRRFLAIIAALSGLAS
                     ATSATAAVKIDPKEIMRVEEVRPGMKGVGRTVFQGVKIEPFDVTVLGVLPKENLGRPL
                     ILVRMDGGPIVKREANIIGGMSGSPVYIKGRLVGAVAYGMTFPREPIAMLTPIEDMLE
                     ALDPALPDKPSWASERHASVPGLLTSPLGPGERELKSPVRIGDRTYERIAFRAQPYHV
                     KDARQPRPGTLEMAPIATPAMVSGISQRSLERLTETFAPFGVQPVSGPGAMKYDKPIP
                     LVPGSAAGASLVVGDIDMTAVGTVTYRKGNQILAFGHPFLQMGAVDLPLTTAWVHDVF
                     PSIHSSFKIASPVQIVGSVRQDRPWGISAHIGQQSDLIPLEATVKDYGSQRRQTIRAR
                     VIDNRFITPQLIAMVANEAIFRIRSVPGDATARVRTRITTDRLGTIERENLYYDALGI
                     DAAALGDLLTALGMLNNNPWERVGIRGVQLDVNIDNSRRTAVIESAVVDRVKYQPGDV
                     VQVNVTLRPWEREPTKRTLSVRIPEYAPNGHVTLRVHGGSASGLPMAAQAGVGGAMMR
                     TAPSPLPAATSLRQMVQQYLEKERNDELVATLALPSFTANIDGQKMSAVPPTLLEVMR
                     NPRNSGVRLQNDEVKASDVTPWVISGMAAVSLTIERKSYSEKAGAPSAPPMPQPQQPQ
                     PAPSPAPGGPQPALEMGLPDAGVLPDGVSGASALSRPDSMVRFAAIQSEPVSPKGEAK
                     LPPTPAGEAAAESKQEQKSGADGKKEEAKKPEVDASAPVKAAGRSPMVWRQESAEAFE
                     RGQLDGVAVSTRGEVTLAPSIQKVCSLDATYVWSL"
BASE COUNT          690 a         1318 c         1368 g          711 t
ORIGIN      
        1 ggtggtcggg gatctgcgag agggggatct tgacctccac ccactcgccc ttcttcagct
       61 cggcaagtga gcgactccag cggcgcgcgc gatcaggcac atgcagcccg aggctggccg
      121 gctcgcgggg cagggtatcg cgtgaggtct cggtgtaaac ccagaagtga aggtagtccc
      181 agcctctcca gtcgcgcagc gggccctcag gaatggtgcg cccgaagcgc ggccatccga
      241 ccggatagtt cggctcgccc gtggtgtggt cgaccgggat gctccagcgc agcgccgtta
      301 gggttgcctt cacgcggtca cgcgaggcct ccacggtgca ttccgcggca gaccactcct
      361 ttgccgtatc acctccgaaa aggagcatct tctccatcgc gttcgcggac gctgccggat
      421 ctgccgaccc cgcggccgcg agacccgaca gcgccatgcc gatgagcagt ggtaacaaac
      481 ggtatcgcat catgcaccct cccaccttgt gcctggattg tgaagcacac cctcctgggg
      541 gaatgcccta cgagtgctcc agcgcggcgc catcttgctt tggcgccgca aatctggcgt
      601 gtctcccgca tctgtcacac ctgcgccagc gccgctctta gctggcgggc gtgttcgtgg
      661 cagaagccgg ggcctttccg atccgtctcc tcaagccctt ccgagaagtg catgacgcac
      721 gaggcaaacg ggcaatgcac gagaccgaag gtatgcccca gttcgtgcac gcactccacg
      781 agcgcccggc gaagaaagag gatgcggttc ggagcctcgc cgcttgcctc cgggtggagg
      841 cggtggagcg agacgatcgc ggttcggccg cccacttctg cttgcccgaa gatgaactcc
      901 atctccgggg cgtagagatc gacctcggtg agggcaagga cgcgcacagc gtctggtgga
      961 gccgcgtggt gcagctcagc gagcatcgcc tcgccgaggt actgttgcct ctcggggttc
     1021 catcccaccg gggcgagttc ctccccgagg cgacgtatcg gcaggccgaa gcggcgtgcc
     1081 aactcgcatc tcaggtgttc ctcgatctcc gcggcgaccg gcccgaagcg tgtcaggcca
     1141 atgaaaccct ttggttcttt catcggtcct cctgtttggg gtgctccgag aaatgccttg
     1201 gacggcccca gacgcctagt tgggaacctg tttcgcgtcc atgcgcgcgt ttcctgtcgg
     1261 gcgaaggtgt ttgcagaggg gcggtctcgt gctattgacg ccatctccag gggagtgata
     1321 tactcctggg aaagcccatg cagagacgaa tgtgtgataa tctctcccgc cgaaaaaccc
     1381 gtgctggggg gccacctgtc tgcttgaccg ggcgcccaac ccgcgcttgc gtatgttggg
     1441 ctcccagcag cgggggaggg gtcacaccca gttcgatgcc tctggctcgt ctcgccgggc
     1501 gcctgtctgg aacgcttctt gcgacatacg gcaagacgtg atgctgagtt gttgtgcatt
     1561 acagcgagcg gtgcccggga gagccgagtg ataccggtcc tgcctctctc gaaccgcgtt
     1621 acttcatttg ggagtgaggg gattgctttg attcaccttt cgtggcgcaa cacccgtcga
     1681 ttcctcgcca tcattgccgc cctttccggg ctggcctcgg ctacctcggc gaccgccgct
     1741 gtcaagattg atccaaaaga gatcatgcgg gtggaagagg tgcgtcccgg gatgaagggc
     1801 gttggccgca cggtgttcca gggcgtcaag atcgagcctt ttgatgtaac cgtgctgggg
     1861 gtgctgccaa aggagaacct gggccggccc ctcatcctgg tacggatgga cggcgggccc
     1921 atcgtcaagc gcgaggcgaa catcatcggc ggaatgagcg gcagcccggt ctacatcaag
     1981 ggccggctgg tgggcgccgt cgcctatggc atgacgttcc cgcgcgagcc gattgcgatg
     2041 ctcacgccca tcgaggatat gctggaggcg cttgacccgg cgcttccgga caagccctcc
     2101 tgggccagtg agcgccacgc cagtgtgccc ggcctgctga cctccccgct gggaccgggc
     2161 gagcgcgagc tgaagagccc ggtgcgcatc ggcgaccgca cctacgagcg gatcgcgttt
     2221 cgcgcccagc cgtatcatgt caaagatgcg cggcagccgc gccctggcac gctggagatg
     2281 gcgcccatcg caaccccggc catggtgtcg ggcattagcc agcgcagcct ggagcgcctg
     2341 accgagacgt tcgcgccctt cggcgtgcag ccggtgtccg gccctggcgc tatgaaatat
     2401 gacaagccga tcccgttggt gcccggctcg gccgcgggtg cctcgctggt agtcggcgat
     2461 atcgacatga ccgccgtggg cacggtcacc taccgcaagg gcaatcagat cctcgccttc
     2521 gggcacccct tcctgcagat gggcgccgtg gatctcccct tgacaactgc ctgggtgcat
     2581 gatgtctttc cgagcatcca cagctccttt aagatcgcct ctcctgtgca gatcgtgggg
     2641 agcgtgcgcc aggatcgccc ctggggcatc agcgcccaca tcggccagca gtcggatctg
     2701 atcccgttgg aggcgacggt caaggattac ggctcgcagc gccggcagac gatccgcgca
     2761 cgcgtgatcg ataaccgctt catcacgccg cagctcatcg cgatggtggc gaacgaggcg
     2821 atcttccgca tccgcagcgt tccgggagac gcgacggcgc gcgtgcgcac gcggatcacc
     2881 accgataggc tgggcaccat cgagcgtgag aacctctact atgatgcgct aggtatcgat
     2941 gccgccgctt tgggggacct cctcacggcc ctcgggatgc tcaacaacaa cccctgggag
     3001 cgcgtgggga tccgtggcgt gcagctcgat gtcaacatcg ataactcccg caggaccgcc
     3061 gtgatcgaga gcgccgttgt cgaccgtgtg aagtaccagc cgggagacgt ggtgcaggtg
     3121 aatgtgaccc tgcggccttg ggagcgggaa cccaccaagc gcacgctctc ggtgcggatc
     3181 cccgagtacg ctcccaacgg gcacgtcacc ctgcgcgtcc atggcggatc ggcatctggt
     3241 cttccgatgg cggcgcaggc gggggtgggg ggcgccatga tgcggacggc gccgtcgccg
     3301 ctgccggccg caacgagctt gcggcagatg gtgcagcaat atctggagaa ggagcgcaac
     3361 gacgagctgg tggcgactct tgcgctgcca tcctttacgg caaacattga tggccagaag
     3421 atgagcgcag tgccgcccac cctcctggaa gtgatgcgca acccgcgcaa cagcggtgtc
     3481 cggctccaga acgacgaggt gaaggcaagc gatgtgacgc cttgggtcat ctctggcatg
     3541 gccgccgtct cgctgacgat tgagcggaag agctactcgg agaaagccgg ggcgccctcg
     3601 gcgccgccga tgccgcagcc ccagcagccg cagccggcgc cctcgccggc gccgggcgga
     3661 ccgcagccgg cgctcgagat gggtctgcct gacgcgggcg tcctgcccga tggcgtctca
     3721 ggcgcatctg ctctctcgcg gcctgattcg atggtgcgct tcgcagccat ccaaagtgag
     3781 cctgtgagcc ccaagggcga ggccaagctg ccgccgaccc ccgcgggtga ggccgctgca
     3841 gagagcaagc aggagcagaa gagcggggca gacgggaaga aggaggaggc aaagaagccg
     3901 gaggttgatg cctcggcacc ggtgaaggcc gccgggcgtt cgccaatggt gtggcggcag
     3961 gagagcgccg aggcgttcga gcgcgggcag ctggacggcg tggccgtctc gacccggggc
     4021 gaggtgacgc tcgcgccctc catccagaag gtgtgctccc tggatgccac ctacgtgtgg
     4081 tctctct
//
LOCUS       DAOSCV010000343         4084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13839_length_4084_cov_3.095061, whole genome shotgun sequence.
ACCESSION   DAOSCV010000343 DAOSCV010000000
VERSION     DAOSCV010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13839_length_4084_cov_3.095061"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PLY56_09375"
     CDS             complement(<1..969)
                     /locus_tag="PLY56_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21732.1"
                     /translation="MVTPSHLDYLRILERWPAYAERFWWNDPARPDLGCFGTGYNSWG
                     VQTNQKYLGAMAILATHPELDEAAAGRSREAILDRALRALRYSLATHVSGDHHCSDGT
                     RWGHTWISALGIERMMHGVEALEEHLTDADRAGLRRVLTSEADAILAMEVQGTKWARD
                     GGNKPESNIWNGAILARVCHMYPDAPRVPDWMEKAHRFLMNGISIAADALDEREVAGR
                     PVREWHVGPNFFDHYALDHHGYLNVGYMVICLSNIAFLHFACTTHGWTPPESLYHHAA
                     DLWDLVKRLVFADGRLLRIGGDSRQRYCYCQDYLIPALLYCAHHLDD"
     gene            complement(1160..3916)
                     /locus_tag="PLY56_09380"
     CDS             complement(1160..3916)
                     /locus_tag="PLY56_09380"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21733.1"
                     /translation="MPVPGGVKLSEADGLLTIDTGAMVATVSTKHGRIVEEVRRGSTV
                     VKPASEVWDFALEDETGRVVRTRDAIIKETEIVERGPMRALIVRKGSFTDAKGTLVDF
                     RIHLEATAGSDALRVLTHIINREETPEVYLKRWSMDLRRADAATGKVWLSPEETRAAN
                     AGAVLYQHREDTVTWTGSDGARSRAKGKSPGYVRLPGMAVGTRWFWERYPQAIRFDTE
                     GVRLDFIPEAFDEGDLPKQWLARLREMTERFTVGGVGYPQSPGKMGLFRLAQGEALGQ
                     EILLVMDGRGTDATDASLFAPLAAPLRAVPEPEYTASTGAFGEFHPSDPVRYARYEES
                     VERTYKGILAQRASRREYGFENFGDSTFEWGYGPSYTYWSNSEYDHHNGYALQYLRSA
                     DPRWWDLCEQTARHTRDVSQIHHAAPGNIQLGGPHHHNATSLWMPQHPEQYWIADHTL
                     AGAHPSHAWAEGMIAYWFLTGDPWTKECVDLLADWYCEVVERNQYGAGGQERGPGWAL
                     IAISALTRATGGERIRNAGWIVANWLLDWQDPIRGVVSVPISEQPSYEGGSTFMHGIV
                     GRGLGRWYDVTGDPRVKDAAIGIAEWITTEPMGEPGTFWYKQSPQNSKRYGADGQCLS
                     ALTYAYKLSGDPWFAEVAKALLSRTGADRRSMSWYPQALGHLASILPPISIRVPASSP
                     AAGETAEVKVVLRAPKGNALKGEIALEAGGGAVVQPAAHPVSAPGGEIVEATFRVTLP
                     ESLPAGPAVFPLRASLKGASKPMEATTQIRAADVPLKGAIVVQAEAFVAQEGGAVQIR
                     DDKAGASGRCFSHWDSAGHWLRWRVRVPEAGTYRLLLRYCTGRDTQREVRVDSEAPLK
                     QTLRNSGGFSSVTSNDWAHHVVTGADGKPYLFRLSAGEHTITLTNTDGNGVNLDYLAL
                     VKER"
BASE COUNT          641 a         1378 c         1375 g          690 t
ORIGIN      
        1 atcatctagg tggtgcgcgc aatagagaag cgcggggatg aggtagtcct ggcagtagca
       61 gtagcgctgc cggctatcgc caccgatgcg caggagacgg ccatcggcga aaacgaggcg
      121 cttcaccagg tcccacaggt cggcggcgtg gtgatagagg ctctcgggcg gggtccagcc
      181 atgggtggtg cacgcgaagt ggaggaaggc aatgttgctc aggcagatca ccatgtagcc
      241 cacgttcaga tagccgtgat ggtcgagggc gtagtgatcg aagaagttgg ggcccacgtg
      301 ccactcgcga accgggcggc cggccacctc gcgctcgtcc agggcatcgg cggcgatgga
      361 gatgccgttc atcaggaagc ggtgcgcctt ctccatccag tcaggcacac gcggggcatc
      421 gggatacatg tggcagacgc gcgccaggat cgccccgttc cagatgttcg actccggttt
      481 gttgccgcca tcgcgcgccc atttcgtgcc ctgtacctcc atcgcgagga tggcatccgc
      541 ctcggaggtg agcacgcggc gcaggccagc gcgatccgca tcggtgagat gctcctccag
      601 cgcctccacg ccatgcatca tccgctcgat ccccagggcg gagatccagg tgtgccccca
      661 gcgcgtgccg tcgctgcagt ggtggtcgcc actgacgtgg gtggccagcg agtagcggag
      721 ggcgcggagc gcgcggtcca ggatcgcctc gcgcgagcgc ccggcggcgg cttcatcgag
      781 ctccgggtgg gtggccagga tggccatcgc gccgaggtac ttctgattgg tctgcacgcc
      841 ccagctgttg tagccggtgc caaagcagcc gaggtcgggg cgggccgggt cgttccacca
      901 gaagcgctcc gcataggcgg gccagcgctc caggatccgc aggtaatcca gatgggaggg
      961 ggtcaccatt cgctttcctc ctctcgtggg cgggaacgct ccgcattggt cggcgcccgt
     1021 gtggaaatcg gaaggagcgc gccgtctctc ggggcggctg ataagaaggc ggggagcatc
     1081 ccttcttcgc tcgggtgctc cccgcctgtc gcacagacgc tcagagccga gagtcaggcc
     1141 gggcgatgcc ggccgcgcct cagcgttcct tcacgagcgc caggtagtct agattgacgc
     1201 cgttgccgtc ggtattcgtc agggtgatcg tgtgctctcc ggcggagagg cggaagagat
     1261 acggtttgcc atccgcgccg gtgaccacat ggtgtgccca gtcattgctt gtgacggagg
     1321 agaagccgcc actgttgcgc agtgtctgct tcagaggcgc ctcggagtct actctcacct
     1381 cgcgctgagt atcgcgcccg gtgcagtagc ggaggagcag gcgataggtg cctgcttccg
     1441 gcacgcgaac gcgccagcgc agccagtggc cggcgctgtc ccaatgcgag aagcacctgc
     1501 ccgaggcccc cgccttgtca tcccggatct ggaccgcgcc accctcttgc gcaacgaacg
     1561 cctctgcctg gaccacgatg gctcccttca ggggcacatc cgccgcacgg atctgcgtcg
     1621 ttgcttccat cggcttggac gcgcctttga ggctggcacg cagtgggaag acggccggcc
     1681 cggcgggcag gctctccgga agcgtcacgc ggaaagtggc ctccacgatc tctccgccgg
     1741 gcgcactcac cgggtgcgcc gccggctgga ctacggcgcc accccccgcc tcgagagcga
     1801 tctcgccctt cagggcgttc cccttgggcg cgcgcaggac gaccttcacc tcagctgtct
     1861 ccccggctgc gggggagctt gccggtaccc ggatggagat gggcggcaga atggatgcca
     1921 ggtgaccgag cgcctgggga taccagctca ttgagcgccg gtcggcgccg gtgcgcgaaa
     1981 gcagcgcctt cgccacctcg gcgaaccagg ggtcgccgct cagcttgtag gcgtaggtga
     2041 gcgccgagag gcactggccg tcggcaccat accgcttgct gttctgcggg ctctgcttgt
     2101 accagaaggt gcccggctcg cccatcggct cggtggtgat ccattcggcg atgccgattg
     2161 ccgcgtcctt gacccgtggg tcgcctgtca cgtcgtacca gcggcccagg ccacggccaa
     2221 cgatgccgtg catgaaggtc gagccgccct cgtagctagg ctgctcggag atcggcacgg
     2281 agaccacgcc gcggatcggg tcttgccagt cgagcagcca gttggccacg atccagccgg
     2341 cgttgcgaat ccgctcgccg cccgtcgctc gcgtcagcgc cgagatggca atcagcgccc
     2401 agcctgggcc gcgctcctgc ccacccgcgc cgtactggtt gcgctccacg acctcgcagt
     2461 accaatcggc gagcagatcc acgcactcct tcgtccaggg atcgccggtg aggaaccagt
     2521 aggcgatcat tccctcagcc cacgcgtggc tgggatgcgc accggcgagt gtgtggtcgg
     2581 cgatccagta ctgctccgga tgttgcggca tccagagaga ggtggcgtta tggtgatgcg
     2641 gcccgccaag ctggatattg cccggtgccg catggtgaat ctgagagacg tcgcgagtat
     2701 gccgcgctgt ctgctcgcac agatcccacc agcgggggtc ggcgctgcgc agatactgca
     2761 gcgcatagcc gttgtggtgg tcgtattcgc tgttgctcca gtacgtgtac gaggggccgt
     2821 atccccactc gaaggtgctg tcgccgaagt tctcgaagcc atactcgcgc cggctggcgc
     2881 gctgggccag aatgccctta taggtgcgct cgacgctctc ttcgtaacgg gcatagcgca
     2941 ctggatcgct cgggtggaac tcgccaaacg ctcccgtgct cgccgtgtat tccggctcgg
     3001 gcaccgcgcg cagcggcgcc gccaggggag cgaagagcga cgcatcagtc gcgtccgtcc
     3061 cgcgcccgtc catcaccagg agaatctcct ggccaagcgc ctcgccctgg gccaggcgga
     3121 agaggcccat cttacctggc gactgcgggt agccgacgcc acccaccgtg aagcgctccg
     3181 tcatctcgcg cagccgggcg agccattgct tgggcagatc gccctcgtca aacgcctcgg
     3241 ggatgaagtc caggcgcacc ccttccgtgt caaagcggat cgcctgcgga tagcgctccc
     3301 agaaccagcg cgttcccacg gccatgcccg gcagacggac gtatccgggc gattttccct
     3361 tggcccgcga gcgtgctcca tcggagcccg tccaggtgac cgtatcctcg cgatgctggt
     3421 agagaaccgc cccagcgttg gcggcgcgtg tctcttccgg cgagagccac acctttccgg
     3481 tggccgcatc cgcgcggcgc agatccatgc tccagcgctt caggtagacc tcgggtgtct
     3541 cctcgcggtt gatgatgtgg gtgaggacgc ggagcgcatc cgagcccgcc gtcgcctcca
     3601 ggtggatgcg gaaatcgacg agcgtgccct tggcgtcggt gaaggagccc ttgcgcacga
     3661 tgagcgcgcg catcggcccg cgctcgacga tctccgtctc cttgatgatg gcgtcgcgcg
     3721 tgcgcaccac ccggccggtc tcatcttcca gggcgaagtc ccacacctcg ctcgcgggct
     3781 tcaccaccgt gctgccacgg cgcacctcct cgacgatccg cccgtgcttc gtgctgacgg
     3841 tggcgaccat ggcacccgta tcgattgtca ggaggccatc cgcctcggag agcttcacac
     3901 cgccgggcac cggcaccggg cgacggcggc ccgactcgtc cggctcccca agctccatgc
     3961 gcacacaacg catggcgcgc acgttgccgg cctcgtcgcc gagcacggct ggaagctgca
     4021 gacggcccag gagaagagct ggctggccga ggagctgaac cagtcgcgca acttcaaggc
     4081 ctgg
//
LOCUS       DAOSCV010000344         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13872_length_4077_cov_2.866982, whole genome shotgun sequence.
ACCESSION   DAOSCV010000344 DAOSCV010000000
VERSION     DAOSCV010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_4077_cov_2.866982"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1634)
                     /locus_tag="PLY56_09385"
     CDS             complement(<1..1634)
                     /locus_tag="PLY56_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ21734.1"
                     /translation="MQALSIAAAALLGYLAGSIPFGYLLVKALRGIDIRDYGSHNIGV
                     SNVARVAGKGTAVLCLVLDAGKGLIPVLLAQRMGAGPWGLVLAGTGACVGHAYSLVFL
                     LKEGRFSRGKAVASGLGAVIGFSLLGAIPAGVLGAVLLVWATCLGLFRFMSLASMAGA
                     AAFAVAVWVTPVDLAYRVFGTVIFLFIVWKHKENLGRLIDGTEVRVGEKVPLANIDGD
                     EVACAFVIHPFEMADCFKSRRFRLLAGWLPTGITRRLLRYVRPMKNDVITGITTRDGR
                     RARVYLIGVPLLAEQIKKDEALAVKRAIQAAELAHHLGASVIGLGAFMSVVGEKGAAV
                     QRHSPIPVTNGGSLTAGSVRLGLQALTERLSDQLGTSTVAVVGANGVVGFKICRDLAP
                     AMRRLILVGTNLERLESGAKLLRKVNPLLEITTSVSCDALVDADLIFSATSDPNAVIF
                     SRHLKEGALVFDLGRPADVDPAVLRERPDVKVIPGGTIRMPGEHVKYHIDLGFGPGNI
                     PACLAETIIIALERAYDRVTLGDGTSAENVEYFVRKA"
     gene            complement(1819..>4077)
                     /locus_tag="PLY56_09390"
     CDS             complement(1819..>4077)
                     /locus_tag="PLY56_09390"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOJ21735.1"
                     /translation="SRSIRLNFTGWKRFRCYLQQMSAARQPLGWNQIHGLRFTASGWG
                     NEPHPEAVVCIDDVILTHEGKEVGPRMTDEQLFEALNLEYPGLGEVKAAVAKGDLAAA
                     KRALAQYYRNRQTPKWYFPAGAKADPKPENPDTARADRVLRHELESIGLRHQFGPEID
                     WTFDLTTAPGSKYAPNNEWTWQLNRHADWSALARAYRDTGDERYAREFTAQMMQWVRT
                     CPVPEDAANTARSTWRTIEAGIRAAQVWPDLFYRFLLSPEFTDEALVTMVKSFAEHAM
                     YLLPHPTSGNWLCMEGNGLFHVGVLFPEFKDAAKWRDTAVEWLYAEMENQVYPDGVQI
                     ELSSGYHHVSLSNFLGVFRLAQLNDIALPADYLKRIERMYDFDDYAAMPDRRLPGVQD
                     GGYMDIRRPLGEAARLFPERKEFLWYATDGKEGEPPAKTSYAFPYAGYFIMRSGWEPD
                     ACWMLFDGGPFGYGHQHEDKLNVIVHAYGKLLLVDPGNYQYERSKWRQYFIDSAAHNV
                     VLVDGQPQRRRGRPRSEYVVKEPLPHVWATAPEYDYVEATFDEGFGGEVKQGVAHTRR
                     VLFVKPDLWILCDTLSAKDGKEHSYEALFHLDAPVKADAAGLRLFTGNEDAANLAIAA
                     RPASGLSLKIVEGQQDPLQGWLPDRGLKSAHPAPVAVFTATGGGDTHLLWVLAPARPG
                     APDPVAAIEPLGDNRLSARIRLRDGRAYEVAFSGGSPADLRVGAARGRGRALLVETRP
                     DGQPGRTIVAAP"
BASE COUNT          728 a         1378 c         1321 g          650 t
ORIGIN      
        1 gccttgcgaa cgaagtactc cacgttctcc gcgctcgtgc catcccccag ggtcacccgg
       61 tcataggccc gttccagggc gatgataatc gtctccgcca gacacgccgg gatgttgccc
      121 ggtccgaacc cgaggtcgat gtggtacttc acgtgctcgc ctggcatgcg gatcgtgccg
      181 ccagggatca ccttgacatc ggggcgctcg cggaggaccg ccgggtcgac gtcggccggc
      241 cggcccaggt caaagacgag cgcgccctcc ttgaggtggc gcgagaagat cacggcgttc
      301 gggtcagagg tcgccgagaa gatgaggtcc gcatccacga gcgcgtcgca gctcaccgag
      361 gtggtaatct ccaggagcgg gttcaccttc cgaaggagtt tcgccccgct ctccaggcgc
      421 tccagattgg tgcccacaag aatcagccgg cgcatcgcgg gcgccagatc ccggcagatc
      481 ttgaagccca ccacgccatt ggcaccgacg accgcgaccg tggacgtccc cagttgatct
      541 gagaggcgct cggtgagagc ctggaggccc aggcgcacgc tccccgcggt gagcgagcct
      601 ccattggtca ccgggatcgg tgaatgccgc tggaccgccg cgcccttctc gcccacaaca
      661 gacatgaacg cacccaggcc gatcaccgat gctcccaggt gatgcgccaa ctcggcggcc
      721 tggatcgccc gcttcaccgc gagcgcctca tccttcttga tctgctcggc aagaagcggg
      781 acgccaatga gatagacgcg cgcgcgccgg ccatcgcgag tggtgatccc ggtgatcaca
      841 tcgttcttca tcgggcgcac gtagcgcagg aggcggcgcg ttatcccggt cggcagccat
      901 ccggcgagca gccggaatcg gcgcgactta aagcaatccg ccatctcaaa ggggtgaatc
      961 acgaaggcac acgccacctc atcgccatcg atgttcgcta ggggtacctt ctcgcccacg
     1021 cggacctcgg tgccgtcgat caaccgcccg aggttctcct tatgcttcca gacgatgaag
     1081 aggaagatga cagtgccaaa cacgcgatac gccaggtcga ccggggtgac ccacacagcg
     1141 acggcgaacg cggccgcgcc ggccatcgat gccagcgaca tgaagcggaa caggccgagg
     1201 catgttgccc aaactaggag gacagcgccg agaacacccg ccgggatcgc cccaaggagg
     1261 ctgaagccga tcaccgcgcc caggccggag gcgacggcct tcccgcgaga gaagcggccc
     1321 tccttcagga ggaaaacgag cgagtaggca tgccccacac acgcacccgt gcccgcgagc
     1381 accaatcccc acggtcctgc tcccatgcgc tgggcaagca gaacggggat caggcccttc
     1441 ccggcatcga gcaccagaca gagtacggcc gtgcccttcc cggcaacccg cgcgacgttc
     1501 gaaaccccga tgttgtggct gccgtagtca cggatatcga tgccccgaag ggctttgacc
     1561 agaaggtagc cgaatgggat agaccccgcc aggtagccaa ggagggccgc cgctgcgatg
     1621 gacagcgctt gcatggcacg attttaccac aagcccggag caactgcaag ccccgcatcc
     1681 ccttcatccg cggcatcggc gccggcccgg ggcgcgaggg ccggccccca tcccctggtg
     1741 atcctggcac ggcaggcttg gagagcccct ggcacaagcc tcacgccagt gccccccaag
     1801 ccagggttgg caccgggatc agggcgctgc gacgatcgtc cggccgggct ggccatccgg
     1861 ccgggtctcg accaggaggg cgcgcccacg gcctctcgct gcgccgaccc gcagatccgc
     1921 cgggctcccc ccagagaaag ccacctcgta cgcccgccca tcgcgcaggc gaatgcgcgc
     1981 cgacaatcgg ttgtctccga ggggttcaat ggcggcaacc gggtctggcg cgccgggccg
     2041 cgcgggggcc agtacccaaa ggagatgcgt gtcacccccg cctgtcgcgg tgaagacggc
     2101 cacgggagcc gggtgcgcgc tcttgagtcc gcggtcgggc agccagccct gcaacgggtc
     2161 ctgctgtccc tccacgatct tcaatgagag cccggaggca ggccgggcgg cgatggcgag
     2221 gttggccgcg tcctcgttgc cagtgaagag gcgaaggccg gcggcatccg ccttcaccgg
     2281 ggcatcgagg tggaagagcg cctcatagga gtgctccttg ccgtctttcg cggagagggt
     2341 gtcgcaaagg atccacagat ccggcttcac gaagaggacg cgccgggtgt gcgccacccc
     2401 ctgcttcacc tcgccgccga agccctcgtc gaaggtggcc tccacgtaat catactcggg
     2461 cgcggtggcc cacacatggg gcaacggctc cttgacgacg tactccgatc tcgggcggcc
     2521 gcgccggcgt tgcggttgac catccaccag aacgacgttg tgcgcggcag agtcgatgaa
     2581 gtattgccgc cacttggacc tctcgtactg gtagttgccc ggatccacga ggagcagctt
     2641 gccgtaggca tgcacgatca cgttcagctt gtcttcgtgc tggtggccat agccgaaggg
     2701 cccgccgtcg aagagcatcc agcaggcatc cggctcccag ccggagcgca tgatgaaata
     2761 gccggcgtag gggaaggcat aggaagtttt cgcgggcggc tctccttcct tgccatcggt
     2821 ggcgtaccag agaaactcct tgcgctcggg gaagagccgg gcggcctcac ccagtggcct
     2881 tcggatatcc atatagccgc catcctgcac gccggggagg cggcggtccg gcatcgcggc
     2941 atagtcgtcg aagtcgtaca tgcgctcgat ccgcttcagg taatccgccg gcagcgcgat
     3001 gtcgttgagt tgggcaaggc ggaacacgcc caggaagttg ctcaggctga cgtggtggta
     3061 gccgctggag agctcgatct gcaccccgtc ggggtacacc tggttctcca tctcggcata
     3121 gagccactcc accgccgtgt cgcgccactt ggccgcgtcc ttgaactctg ggaagagcac
     3181 tcccacgtga aagaggccgt ttccctccat gcacagccag ttgccggagg tgggatgggg
     3241 aagaagatac atcgcgtgct cggcgaacga tttgaccatc gtcaccagcg cctcgtcggt
     3301 gaactccggc gagaggagga agcggtagaa gagatccggc caaacttgcg cagcgcggat
     3361 acccgcctcg atggtgcgcc aggtcgagcg cgcggtgttg gcggcgtcct ccggcaccgg
     3421 gcaggtgcgc acccactgca tcatctgcgc cgtgaactcg cgcgcgtaac gctcatcgcc
     3481 cgtatcacgg taggcccgag ccagagcaga ccaatcggcg tggcggttga gctgccaggt
     3541 ccactcgttg ttcggggcgt acttcgatcc gggcgctgtg gtcaggtcga acgtccaatc
     3601 gatctccggc ccgaactggt ggcgcaggcc gatgctctcc aactcgtggc ggagcacccg
     3661 gtcggcacgg gcggtatctg gattctccgg ctttgggtcg gccttcgctc cggctgggaa
     3721 gtaccacttc ggcgtctggc ggttgcggta atactgcgcc agggcgcgct tcgccgctgc
     3781 caggtcgccc ttcgccaccg cggctttcac ctcgcccaac cccggatact ccaggttcag
     3841 ggcctcgaaa agctgttcat cggtcatgcg gggccctacc tccttcccct catgcgtcag
     3901 gataacgtcg tcgatgcaga cgacggcctc ggggtgcggc tcattccccc acccagaggc
     3961 ggtgaagcgc aacccgtgga tctgattcca cccgagcggc tggcgagccg cgctcatctg
     4021 ctgcaggtag cagcggaagc gcttccagcc agtgaagttg aggcggatgc ttcgcga
//
LOCUS       DAOSCV010000345         4074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13885_length_4074_cov_4.182632, whole genome shotgun sequence.
ACCESSION   DAOSCV010000345 DAOSCV010000000
VERSION     DAOSCV010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_4074_cov_4.182632"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLY56_09395"
     CDS             <1..251
                     /locus_tag="PLY56_09395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017548218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21736.1"
                     /translation="VAVHPEFQGRKLAKPLVGAAMHRLAELHTRAYLTTQTTSWKAVK
                     IYLDFGFVPLMETPRCEDAWRLLAEITGHPALTQFRGR"
     gene            complement(264..956)
                     /locus_tag="PLY56_09400"
     CDS             complement(264..956)
                     /locus_tag="PLY56_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ21737.1"
                     /translation="MLDARSSREEVSASSWEGLLSALRRGEHERARALASRIDQEICA
                     RSGESRAHTRRRALRLALDLADAAVQGGVPAERADAAVLRAAGEIVACTEDDLGGAMR
                     RLLDALLLAPTAPGQSAARAVRQAVEYIQRNFRRSLTLPEVARHVRLSPAYFSVIFKR
                     ERGIGFVRFLRELRLAEARRLLRETGDTVEAIANAVGYDDVHYFSRVFTREMGVPPGR
                     YREQYQESAARE"
     gene            1608..1919
                     /locus_tag="PLY56_09405"
     CDS             1608..1919
                     /locus_tag="PLY56_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21738.1"
                     /translation="MRAEQRMRLRAALFPAVARVRLQMRPLRRQAEELAALVRSTDYR
                     SIDLDDLTARVRHFHASVREFSDTALPAMDEALEDVRAILQEESPERAPCQAPSDSPM
                     P"
     gene            2206..>4074
                     /locus_tag="PLY56_09410"
     CDS             2206..>4074
                     /locus_tag="PLY56_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21739.1"
                     /translation="MNCERFFLGREEPYLHRVVRLLWDQYARAGFWDLSGLVVAVPGG
                     RAGRRLAELLTEAAARNGEGHTALLLPRIVTAGDLPEELYSCQEPVADGLTALLALAA
                     ALRGLGRERLAPLVPRPPESDSLLDWVSLAREVERLRTDLASEGLRVTDAARRCAALP
                     GFHDGARWEVLSAVEEAYRDLLAGQGWIDRHEARFWALEQESLEPSGPVLLVATVDLP
                     QITRQILSRSGATVTALIPASPEEADGFDELGCLVADYWIDRHVMIPDSALRIVDRPV
                     DQSLALLDALAELEGTRTPDEITVGVGDEKLAPALERALRLSGVPVRSIFGRPLTSTR
                     PALLLGALRDYLASRRTEDFAALLRHPDLEAYLYRRAKEDDSRIDLWRAPDRFDAYVS
                     EHLPLHLDPTREDLPGEIATLCRWIGELAAELLSEPCPLSEWSARLLQVLRDVYAPLL
                     LDPGDATDRQLLRALRALVEQLENQSRLPAEGPLAARVSFSEAISFTLGQLSGAVLPQ
                     ESDQPAVELLGWLELALDDAPVLIVTGVNEGAIPASVQVDPFLPDSLRRSLGLVDNRH
                     RYARDLLLMTVILQTRPQVTLIAGRRDADGDPLTPSRLLFACDEEPPRRQRVAVRGP"
BASE COUNT          620 a         1395 c         1325 g          734 t
ORIGIN      
        1 gggtcgcggt ccaccccgag tttcagggca ggaagctcgc gaaacctctg gtcggcgcgg
       61 cgatgcaccg cctggcggag ctgcacaccc gggcctatct caccacgcag acaaccagct
      121 ggaaagccgt gaagatctac ctcgacttcg gcttcgtgcc cctgatggag acgccacgct
      181 gcgaggatgc ttggcggctc ctcgccgaga ttaccggcca ccccgcgctg acccagttcc
      241 gtggtaggtg aacggggcgc aggtcattct ctcgccgcgc tctcctgata ctgctcccgg
      301 tatctccctg gtggcacgcc catctcgcgc gtgaagacgc gtgagaaata gtggacgtcg
      361 tcatacccga cggcgttcgc gatcgcctcc acggtgtccc ctgtctcgcg gagcagccgc
      421 cttgcctcgg ccaggcgcag ctctctcagg aagcggacaa agccgatgcc acgctcccgc
      481 ttgaaaatca cgctgaagta ggcaggactc aggcgcacgt gtcgggcgac ctcgggcagc
      541 gtcagactgc gccggaagtt ccgctggata tactccaccg cctgtcgaac ggctcgggcg
      601 gcgctttgcc ccggagccgt aggggcaagg agcagggcat ccagcagccg gcgcatggcc
      661 cctcccagat cgtcttccgt gcaggcgacg atctcgcccg ccgcgcgtaa caccgccgca
      721 tcggcgcgtt ccgccggaac tcccccttgc accgccgcgt cagcgagatc gagcgccagt
      781 cgaagcgcgc gccggcgcgt gtgcgcccgg ctctctccac tgcgagcgca tatctcctgg
      841 tcgatgcggg atgccagggc acgcgctctc tcgtgctctc cccgacgcaa cgcgctgagg
      901 agcccctccc atgaggaagc cgatacctcc tctcgcgaac tcctggcatc aagcacgtgt
      961 gcccatccct ccagtgcctt tcgtgggagt tccgaattca cgagcacgtg gatgcctggg
     1021 ccgcccgctt ttccccgact tctccttgtc tccactccgc cggcgccggg gagaggagtt
     1081 gcgagcgaat ggaagcgaga tgctcagagt ggatctggtc gcgcgctgct cccaccgccc
     1141 acggatctag ggggaacggc acgggatgtg gggaggggag acgggagagc gcatcggcca
     1201 acctccctgc caggccctgt actcatgcaa tcgtgaacga tgactcctct ttcaccgtag
     1261 catagcggcc ggggggatgt caaggcgtgc gcagcggctc tgggattccg agataaggag
     1321 aaggctgccc gcacgcgcgg agctcgaagc tccgcgctgc tttccggcaa agccccttcg
     1381 ggcttctcct cccagccagc gccgttttga gacatctggg agtatcccca acccagcccg
     1441 gcagggcttc gctcgcaagc agcgcggtga tttatcgccg cgcgccggct tgcacgctgc
     1501 atccgttgct tcggattcct tatctcggaa tcccagcagc ggctatagaa atgagcggaa
     1561 agcgccgaag ggggggataa ggcccgtggg ttggcaggga gatagcgatg agagctgaac
     1621 agcgaatgcg ccttcgcgcg gcgctcttcc cggcggttgc gcgtgtgcgc ttacagatgc
     1681 gcccgctgcg ccgccaggca gaggagcttg cggcgttggt ccgaagcact gactacagga
     1741 gcattgacct cgacgatctg accgcgcgag tacgtcactt ccacgcgagc gttcgggagt
     1801 tcagcgatac cgcgctcccg gcgatggatg aggcgctgga ggatgttcgc gccatactcc
     1861 aggaagaatc ccctgaaagg gcgccgtgcc aggcgccttc tgattccccc atgccttgag
     1921 cgggcacgag ggtgtgcgcg ggcgtttgcg cccgcgcctg tcggcccggc aaaacgtcgc
     1981 gtgggcataa gcatccgcgc cccattggca cgtgcgtggt acccgcgcgt ccatgcatcc
     2041 ccaggtgacc cctcgaaaag gggactaggg cgtgcttatc gaacggcttg tccagctgag
     2101 gcatgagtta acgctcgctc gtgcctcggg cgcatgcaag ccggagaagc tccgaatggc
     2161 ccggctcgcc cccctacgtg cgcatcctcc agaggggaac gactgatgaa ctgcgaacgc
     2221 ttctttctcg gccgcgagga gccctacctc caccgggtgg tgcgcttgct gtgggaccag
     2281 tatgcccgcg ctggcttctg ggacctgagc gggctcgttg tcgccgtgcc gggtggccgc
     2341 gctggccggc gcctggcgga actgctgacc gaagcggctg cccgcaatgg ggaggggcac
     2401 acagcgctcc ttctcccgcg aattgtgacc gccggcgacc tccccgagga gctctattcc
     2461 tgccaggagc ccgtggccga tggtctcacc gcgcttctgg cgctggccgc cgcgctgcgc
     2521 ggcttgggcc gcgagcgcct ggcccccctc gttccccggc ccccggaatc cgacagcctc
     2581 ctcgattggg tgtcactcgc gcgcgaggtg gaacgcctgc gcacggatct ggcgagtgag
     2641 ggcctgcgcg tgacggatgc cgctcgccgg tgcgccgctc ttcccgggtt ccatgatggc
     2701 gcgcgttggg aggttctttc cgccgtggaa gaggcctacc gcgatctcct ggccggacaa
     2761 ggctggattg accggcatga agcacgtttc tgggcgcttg agcaggaaag cctggagcct
     2821 tctggcccgg tcctgctcgt cgccacggtg gacctgcctc agatcacgcg ccagatcctc
     2881 tcgcgcagtg gggctaccgt gacggcgctt attccagcgt ccccagagga agccgatggc
     2941 ttcgatgagc tcgggtgtct cgtggcggac tactggatcg accggcacgt gatgattcca
     3001 gacagtgcgc tgcggatcgt cgaccggccg gtggatcagt ccctcgcgct tcttgatgcc
     3061 ctggccgaac tcgaaggcac gcgtaccccg gatgagatca ccgtgggcgt gggtgatgaa
     3121 aagctcgcgc ctgccctgga gcgcgcgctg cgcctgagcg gcgtgcccgt gcgctccatt
     3181 ttcggccgtc ccctcacatc gacgcgcccg gctctgctcc tgggcgcatt gcgcgattac
     3241 ctagcctccc gccggaccga ggactttgcc gcgctcttgc gccatccgga tctggaagcc
     3301 tatctctacc ggcgcgctaa ggaggacgat agccgcatcg atctctggcg ggctcccgac
     3361 cgctttgatg cctacgtctc ggagcatctg cccctccatc tggatcccac ccgtgaggat
     3421 ctccctggcg agatcgccac tctctgccgg tggatcggag agctggcggc ggaactactc
     3481 tcggaaccgt gtcccctttc ggagtggtcc gcgcgcttgc ttcaggtgct tcgggatgtc
     3541 tatgcgccgc tgttgctcga tccgggtgac gcgacagacc gtcaactcct tcgtgcgctg
     3601 cgcgcgctgg tggaacagct ggaaaaccag agccggctgc ccgcggaggg gcctttggcc
     3661 gcccgtgtct ccttctcgga ggcgatctcc ttcaccctcg ggcagctctc gggagccgtg
     3721 cttccacagg agagcgatca acccgccgtc gagctgctcg gctggttgga attggcgctc
     3781 gatgatgccc ccgtcctgat cgtcacgggc gtgaacgagg gtgccattcc tgccagcgtt
     3841 caagtggatc ccttcctccc ggatagcctc cggcgctccc tgggactggt ggataaccgt
     3901 caccgctacg cgcgcgacct gctgctgatg acggtcatac tccagacgcg cccccaggtg
     3961 acgctgatcg cgggccgtcg ggacgcggat ggcgacccgc tcaccccgag ccggctcctc
     4021 ttcgcctgcg atgaggagcc gcctcggcgg cagcgggtcg ccgtccgcgg cccg
//
LOCUS       DAOSCV010000346         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13919_length_4067_cov_3.768943, whole genome shotgun sequence.
ACCESSION   DAOSCV010000346 DAOSCV010000000
VERSION     DAOSCV010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13919_length_4067_cov_3.768943"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PLY56_09415"
     CDS             <1..1599
                     /locus_tag="PLY56_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21740.1"
                     /translation="SILWNGAYVGDRVRIDGAVVGRNCSIDRGSELLEGAVIGDRTHI
                     QRSSLVFPHIKIWPDKTVEAGARVTESLVWGPRWSRTLFSEGGVSGLANIEMTPDFAT
                     KLATAYGSYLGRGATVIISRDAHPTSRMIKRALASGLMSAGINVQDLRSIPVSVSRFL
                     LAESGARGGVHVRISPHDPRMITVELCNAKGAILDKNAQRKVENIFFREDFVRADFVG
                     VGTLEYVPWTVERYTERLTELVDCKACHDSRFRVMLDCAYGGASAVLPSALGALGCDL
                     VTLNGFSRPLEEPRTAEEKERSVLELSNLVKSVEAEIGLLVHNDGETMHVTDHTGRPI
                     ADSMLLSIMCMLAFATGDARVVAVPASAPSVIEQIAEGFRGRVIRTKSDPAALMGLAL
                     ADRDGVDLIGDTEGRFIFPRFHPAFDAMISCAKLIEYLARSGKRRLADVVALVPRYHL
                     RRADVSCPWEKKGEIMRRLTEEAEQGRADFTEGVKVITDRGWVLVSPDAFEPTFHIYA
                     EAGSPEHATELIVRFREKIETLCS"
     gene            1800..3104
                     /locus_tag="PLY56_09420"
     CDS             1800..3104
                     /locus_tag="PLY56_09420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ21741.1"
                     /translation="MGEGPLLRPVLPENTEFVILSFEGPDPYSNAGGLGVRVTELSRA
                     LADAGFRTHLFFIGDPEKPPIEAGGGRLILRRWGQWISRYYPNGVYQGEKQKLYDFHS
                     SVPGVIADEVVAPARREGRTVVIMGEEWHTASTMCAISDLLHARGQRDQAILLWNANH
                     TMGFENIDFGRLNFTTTITTVSHYMKHTMWLWGINPIVLPNGIPDRLLSAPPAEEVQA
                     IRDVVAGDPMLVKVGRWDPDKRWNMAVHAVAGLKRMGLRPRFVCRGGIEPYEGEVLQT
                     ARSLGLSIYDVPKPETQDLAGWCAALADTRDADIINLKFFVPEHLLRLLYAGADAVLA
                     NSGKEPFGIVGLEVMASGGIAVTGATGEEYARAFENAIVLETDNANEIVTSLAYLREH
                     PEEAARIRAQARVTAAEYTWARVVELLCKRIQLLSTSQNLSR"
     gene            3127..>4067
                     /locus_tag="PLY56_09425"
     CDS             3127..>4067
                     /locus_tag="PLY56_09425"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOJ21742.1"
                     /translation="MAEHLVTYTVVHQPRRIKLPAQPIPHGATPEDIARCLFDERMNE
                     RYLHKVAEYCYYPATRMFLEMVQEQDFRLAIGFSLSFLRQAQMWDPDLLALMKKLVAH
                     ENVELVCVEPYHSFLFYMDIGLFIERMKKTREELYALFAKRPVVTDTTEMFISNDIYF
                     ALDQAGFHAAVLDGREWVMNWRETTHLYNYAGKSLRLLPRHYKLSDDVGYRFSNKGWE
                     GWPLQAGDYAHWIREAMGEFVFLAWDYETFGEHHSRDTGIFEFMRCLPGELKFRGVQP
                     LTPSQALERFADRVYELPLPQFPSTWAGSGGSEFFLG"
BASE COUNT          717 a         1323 c         1298 g          729 t
ORIGIN      
        1 agcatcctct ggaacggcgc ctatgtgggc gaccgcgtcc gcatcgatgg cgccgtggtc
       61 ggccgcaact gcagcatcga tcgcgggagc gagctcctcg aaggcgcggt gatcggcgac
      121 cgcactcata tccaacgcag cagcctcgtc ttcccccaca tcaagatctg gccagacaag
      181 acggtggagg caggcgcgcg cgtgacggaa agcctggtat gggggccgcg ctggtcacgc
      241 accctcttct ccgagggcgg cgtcagcggc ctggcgaaca tcgagatgac ccccgatttc
      301 gcaaccaagc tcgcgaccgc ctatggctcc tacctggggc gcggcgccac ggtgatcatc
      361 agccgcgatg cccacccgac cagccgcatg atcaagcgtg cgctggcctc cggcttgatg
      421 tccgcgggca tcaacgtcca ggatctgcgc tccatccccg tctctgtctc ccggttcctg
      481 ctggcggagt ccggggctcg tggtggcgtg catgtgcgga tctcgcctca cgacccacgc
      541 atgatcaccg tcgagctgtg caacgccaaa ggtgccattc tcgataagaa tgcccaacgc
      601 aaggtggaga atatcttctt ccgcgaggac ttcgtgcgcg ccgatttcgt gggggtgggc
      661 acgctcgagt acgtgccgtg gacggtcgag cgctacaccg agcgcctcac cgagctcgtg
      721 gattgcaagg cgtgccacga ttcgcgcttc cgcgtgatgc tggactgcgc ctatgggggc
      781 gccagcgcgg tgctcccctc ggctctgggt gccttggggt gcgacctggt cacgctcaac
      841 ggcttctcac gcccgctcga agagccacga accgcggagg agaaggagcg cagcgtcctc
      901 gagctgagca acctggtgaa gagcgttgag gcggagattg gcctgctcgt ccacaacgac
      961 ggcgagacga tgcatgtcac agatcacacc ggccggccaa tcgccgactc gatgcttctt
     1021 tcgatcatgt gcatgctcgc gttcgccacc ggcgacgcgc gcgtcgtggc ggttccggcg
     1081 tccgcgccct cggtgatcga gcagatcgca gagggcttcc gagggcgcgt gatccgcacg
     1141 aagagcgatc ccgccgccct gatgggcctg gccctcgcgg atcgggacgg ggttgacctg
     1201 atcggtgata ccgaaggccg ctttatcttc ccgcggttcc atccggcgtt cgatgctatg
     1261 atctcctgcg ccaagctgat cgaatatctg gcgcgctccg gcaagcggcg tctggccgac
     1321 gtggtggcgc tggtgccccg ctatcacctg cgccgggccg acgtaagctg cccctgggaa
     1381 aagaagggcg agatcatgcg ccgtctcacc gaagaggcgg agcaaggtcg cgccgatttc
     1441 accgaagggg tcaaagtgat caccgataga gggtgggttc tggtctcgcc ggatgccttc
     1501 gagcccacct tccatatcta cgcggaggcg gggtcgcccg agcatgccac ggagctgatc
     1561 gtccgcttcc gcgagaagat cgagaccctc tgttcgtagc gaacgcagcg gggcgagcgg
     1621 acggctgctt ggataaccga cgcccgcccc gcgcggctgc ctcgcaagga gctccctggc
     1681 attccgaacg gtctgcgcaa cgggagtggc tcgggcgagt ccggcgcgcc gtgccggcgc
     1741 cgggcacgcc cgcgtgacac tctcggctgt gaacagatat gaggaaggaa gagcgtaaca
     1801 tgggtgaagg tccgctcctc cggcccgtct tgccggagaa tacggagttt gtgatcttat
     1861 ccttcgaggg cccggatccc tattcgaatg cgggcgggct aggcgtgcgc gtcaccgagc
     1921 tgtcgcgcgc gctcgcagac gcagggtttc gcacacacct cttcttcatt ggcgatccgg
     1981 agaagccgcc catcgaggcc ggcggcggtc gcctcatcct gcgccgctgg gggcagtgga
     2041 tcagccgcta ctacccgaat ggcgtctatc agggcgagaa gcagaaactg tatgacttcc
     2101 actccagcgt gcccggggtg atcgcggatg aggtcgtcgc ccccgcccgg cgcgagggcc
     2161 gaacggtcgt tatcatgggc gaggagtggc acacggcgtc cacgatgtgc gccatcagcg
     2221 acctcctcca tgcccgcggg cagcgcgatc aggcgatcct cctatggaac gccaaccaca
     2281 ccatgggctt cgagaatatc gacttcgggc gcctgaattt caccaccacg atcaccaccg
     2341 tcagccacta catgaagcac accatgtggc tgtggggcat caaccccatc gtcttgccca
     2401 acggcatccc cgaccgcctg ctgagcgcac cccccgccga ggaggtgcag gccattcgcg
     2461 atgtggtggc cggcgatccg atgctggtca aggtggggcg ctgggaccca gacaagcgct
     2521 ggaatatggc ggtacatgcc gttgcaggtc tcaagaggat ggggctgcgc ccgcgcttcg
     2581 tctgccgcgg cggcatcgag ccgtacgagg gagaggtgct acagacagcg cgctcgctgg
     2641 ggctctccat ctacgacgtt cccaagccgg agacgcagga cctggcagga tggtgcgccg
     2701 cccttgccga cacgcgcgac gccgatatca tcaacctgaa gttctttgtt ccagagcacc
     2761 tgctccgcct gctctacgcc ggcgcggacg cggttctcgc caacagcggc aaggagccgt
     2821 tcgggatcgt cggtctggag gtgatggcca gcggcggtat tgccgtgacg ggcgccacag
     2881 gcgaagagta tgcccgggcc ttcgagaacg ccatcgtcct cgaaacagat aacgcgaatg
     2941 agatcgtcac ttcgctcgcc tacctccgcg agcatccaga ggaggccgcg cgcattcgcg
     3001 ctcaggcgcg cgtcaccgcc gcggagtata cgtgggctcg ggtcgtggaa ctgctctgca
     3061 agcgtattca gctgctctcc acgagccaga atctctcgag gtagttccct gagaggagga
     3121 atccccttgg ctgagcatct cgtgacctac acggtcgtcc atcaaccgcg ccgtatcaag
     3181 ctgcctgcgc agccgattcc ccacggtgcc acacccgagg atatcgcgcg ctgcctcttc
     3241 gacgagagga tgaacgagcg ctacctgcat aaggtggcgg aatactgcta ctatccggca
     3301 acccgcatgt tcctcgagat ggttcaggag caagacttcc gtctcgccat cgggttctcg
     3361 ctctccttcc ttcgccaggc ccagatgtgg gacccagatc tcctggcgct gatgaagaag
     3421 ctcgtcgccc acgaaaacgt cgagttggtg tgcgtggagc cctaccactc cttcctcttc
     3481 tatatggata tcgggctgtt catcgaacgg atgaagaaaa cccgagagga actctacgct
     3541 ctctttgcga agcgccccgt cgtcaccgac acgaccgaga tgttcatctc gaatgacatc
     3601 tacttcgcgc tcgaccaggc tggcttccac gcagccgtgc tcgatggccg cgagtgggtc
     3661 atgaactggc gagagacgac gcacctctac aactacgccg gcaaatcgct ccgtctcctg
     3721 ccgcgtcact ataagctgag cgatgacgtc ggttaccgct tctccaacaa gggctgggag
     3781 ggctggccgc tgcaagccgg ggattatgcc cactggatcc gcgaggcgat gggcgagttc
     3841 gtcttcctgg cgtgggacta cgagacgttc ggcgagcatc actcgcgcga caccggcatc
     3901 ttcgagttca tgcgctgtct gcctggcgag ctgaagttcc gaggcgttca gccgctcact
     3961 ccttcccagg cgctggagcg cttcgcggac cgggtgtacg agctgccgct gccgcaattc
     4021 cccagcacct gggcaggaag cggcgggagc gagttcttcc tggggaa
//
LOCUS       DAOSCV010000347         4067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13920_length_4067_cov_3.428215, whole genome shotgun sequence.
ACCESSION   DAOSCV010000347 DAOSCV010000000
VERSION     DAOSCV010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13920_length_4067_cov_3.428215"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PLY56_09430"
     CDS             complement(<1..754)
                     /locus_tag="PLY56_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21743.1"
                     /translation="MKSVKYGVIGLGWFGEKHCEALAAIPNVELYALCTRTPSRLAEV
                     SERFGVQRTYTDYNEMLADPELEAVSVVTMWDQHRAPAVAALKAGKHVFLEKPMASTV
                     EDCDAIVAAAKATDKFFMVGHICRFNPRYAAAKAAIAEGKIGKIVSMYARRNIPAWVG
                     ADVLPKIGPIIGDGVHDTDLMLWYSGAKVVSAYAQSLNVRGFKHPDLGWTMYRFDTGA
                     IGVLENVWFLPDKTAFQIDERMEIIGTEGSIHI"
     gene            1152..2408
                     /locus_tag="PLY56_09435"
     CDS             1152..2408
                     /locus_tag="PLY56_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOJ21744.1"
                     /translation="MWVLLALYLVLSSIVMPGAHAKEAGAFAQVDAHAEKAPPSAEKS
                     VSALAKYLVRQARNDEEKARAIFRWITLNVSYDTARYQSGAPGAMSPEEVLHRRKAVC
                     SGYAALFEHLAKEAGLEAVTIRGHSKGYPISASERQDSDLNHAWNAVKIDGKWRLLDC
                     TWGAGHVTPSMKFVRKFSEHYFLTPPEEFLYGHFPLEPKWQLLDRPVARHEYERMVPL
                     RPDFFRCGLALHSHRWASFEAEDSATVTLTGPEDALLLCNLMRDGKELGRSYTLTQRE
                     KGKIVTRVRFPRPGNYILRYFVKRRNESGPYSWALDYQITASRGAKERAEFPKTYGTF
                     QESGAILEEPMVWRLKAGERQRFSLRVPGAAAVAVSVDGKWTHLQRTGDRFTGEVALR
                     PGPVQLCASLDPAKRSYSVLLEYEAK"
     gene            2405..3157
                     /locus_tag="PLY56_09440"
     CDS             2405..3157
                     /locus_tag="PLY56_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21745.1"
                     /translation="MSLHLGAWDVALVVAVSAQATVLAYLYAPRWKALVWMLPIPFTM
                     ASLAVGRPVDATNVLGLPLLWVFTHGVRVLHLRARLPIVAAIVVSTAVYIAGGWLLAD
                     IVPEGDTAFWISACAMLLIGWVLQRIEPSRDEPGYRSPLPLRYKLPAIIAVVTALLFI
                     KRGLAGFTTLFPMVGVIAAYEARYSLHTMARTVPGIMLTLVPLMMVCRLLQERIGLGP
                     ALAVGWVVYLVLLTRLTRSTWKPQADSCASQR"
     gene            3189..3959
                     /locus_tag="PLY56_09445"
     CDS             3189..3959
                     /locus_tag="PLY56_09445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ21746.1"
                     /translation="MKLLALDVGNTNIVLGIFEDQRLVASWRLSTDRGRTADEYGVLA
                     TLMLAQAGITPKDVGGIAVCSVVPPLDHTLRTLLTRTFDAPILTVDHSTDTGIRICYE
                     TPSDVGPDRIVNAAAAYHQVGGPCVVVDFGTATTFDVVTANGEYHGGAIAPGIGISME
                     ALFARAARLPRIDLARPPRVIGKTTRESMQSGFVFGFAGQADAIISRLCAELGPETRV
                     VATGGLAELIAPETQSIRHVRPELTLEGLEIIWRRQQR"
BASE COUNT          683 a         1349 c         1316 g          719 t
ORIGIN      
        1 ggatatggat cgaaccctcg gtgccgatga tctccatgcg ctcgtcgatc tggaacgccg
       61 ttttgtccgg caggaaccac acattctcga gcacgccgat agcgcccgtg tcgaagcggt
      121 acatcgtcca ccccaggtcc ggatgcttga agccgcggac gttgagcgac tgggcgtagg
      181 ccgaaaccac cttcgcgccc gaataccaga gcatgaggtc ggtatcgtgg acaccgtccc
      241 caatgatcgg gccgatcttg gggaggacat cggcgccgac ccacgcggga atgttgcgcc
      301 gggcgtacat cgagacgatc ttgccgatct tgccctcggc aatcgccgcc ttcgcagcgg
      361 catagcgcgg gttgaagcgg cagatatgcc ccaccatgaa gaacttgtcg gtggccttcg
      421 ccgcggccac gatagcatca cagtcctcga cggtggaagc catcggcttc tcaaggaaga
      481 cgtgcttgcc tgccttcagc gcagccacgg cgggcgcccg gtgctggtcc cacatcgtga
      541 ccacgctgac ggcttccagc tctgggtcgg cgagcatctc gttgtaatcc gtgtaggtgc
      601 gctgaacccc aaaacgctcg gacacttcgg caagccggga gggcgtgcgc gtgcagagcg
      661 cgtacaactc cacgttggga atggctgcca acgcctcgca gtgcttctcg ccgaaccatc
      721 ccaaaccgat caccccatac ttgaccgact tcatcccatg acctcctgtc tcagtaccga
      781 ccggacccgg tctcgaccgg ggaaggcttg cccggctatt ccgcatccag ccaccgagat
      841 cctcctgctc ctccgcatcg acagaggcat cgatcctgca cgcgacgctc tgagaggatt
      901 ccccggcacc ggaccggaat ggtagcgtac ggcctcgccc gctgccgcac cggcagccgg
      961 gagccactat ccgcgctcac cccagcggcg gtcggggagc acctcgcacg gggcgctgag
     1021 ccgcgggcag cctgcaaggc ggaaccaact tcacgcctcg cctggcctca gcgagcagaa
     1081 gattccctta tcgcgcgcag agcagactcg cccacggggc ctggaaagcg cgggtgagca
     1141 gggaggaagg gatgtgggtg ctgcttgcac tctatctggt gctgtcatca atcgtcatgc
     1201 cgggcgcgca tgccaaggaa gccggggcct tcgcccaagt ggatgcgcac gccgagaagg
     1261 caccgccttc agcggagaag agcgtgtctg ccctggcgaa atacctggtt cgccaggcca
     1321 ggaacgacga ggagaaggcg cgcgcgatct tccgatggat caccctcaat gtgagctacg
     1381 acaccgcccg ctatcaatcg ggagcccccg gcgctatgag tccggaggag gtgctgcacc
     1441 ggcgcaaggc cgtctgctct ggctacgccg cgctcttcga gcacctggct aaggaggccg
     1501 ggcttgaggc ggtgacgatc cgcgggcact cgaaggggta tcccatctcg gcgagcgagc
     1561 gtcaggatag cgacctcaat cacgcctgga acgcggtgaa gatcgatgga aagtggcgtc
     1621 ttctggactg cacctggggc gccgggcacg tcacaccttc gatgaagttc gtccgcaagt
     1681 tctcggagca ttacttcttg acgccgcccg aggagttcct ttacggccac ttcccgctcg
     1741 agccgaagtg gcagctcctc gaccggccgg tcgcccgcca cgaatatgag cgcatggttc
     1801 cccttcgccc ggacttcttc cgctgcgggc tggccctgca cagccaccgc tgggcgtcgt
     1861 tcgaggctga ggattcggca accgtgaccc tcaccggtcc tgaggatgcg ctgctcctgt
     1921 gcaacctgat gcgcgatggg aaggagctag ggcgctccta cacgctcacg cagcgggaga
     1981 aggggaagat cgtcacgcgg gtgcgcttcc cgcggcctgg caactacatc ctccgctatt
     2041 tcgtgaagcg acggaacgag tccgggccct attcctgggc gctcgactat cagatcaccg
     2101 cctcgcgtgg cgccaaagaa cgggcggagt ttccgaagac ctacggcact tttcaggaga
     2161 gcggcgccat cctggaagaa ccgatggtct ggcggctgaa ggcgggggag cgccagcgct
     2221 tctcactgcg ggtgccgggt gccgccgcgg tcgctgtctc cgttgacggg aagtggaccc
     2281 acctccagcg aacgggcgac cggtttacgg gcgaggtcgc gcttcggccg ggcccggtgc
     2341 aactctgcgc cagcctcgac cccgcgaagc ggagctactc tgtgctcctg gagtatgagg
     2401 cgaagtgagt cttcatctgg gcgcctggga cgtggcgctg gtggttgcgg tctcggcgca
     2461 ggcaacggtc ctggcctacc tgtacgcccc gcgctggaag gcgctggtct ggatgctccc
     2521 cattccgttc accatggcgt cgctcgccgt gggccggccc gtggacgcga ccaacgtcct
     2581 cgggctgcca ctactgtggg tcttcactca tggggtgcgc gtgctccacc tgcgggcgcg
     2641 tctcccgatc gtggccgcca tcgtggtctc gacggcggtc tacatcgccg gtgggtggtt
     2701 actggcggat atcgtgccgg aaggcgatac ggcgttctgg atctccgcgt gcgccatgct
     2761 gctcatcggc tgggtgctcc aacgcatcga gccgagccgg gatgagccgg gctaccgcag
     2821 tcccctgccc ctccgataca agctgcccgc gatcatcgct gtggtcactg cgctgctctt
     2881 catcaagcgt ggcctggccg ggttcaccac gcttttcccg atggtggggg tgatcgccgc
     2941 ctacgaagcg cgttacagcc tccataccat ggcgcggacg gtgcccggga tcatgctcac
     3001 cctggtgccc ctcatgatgg tgtgccgctt gctgcaagag cggatcggtc tgggccccgc
     3061 gctggccgtc ggctgggtcg tctacctggt tctcctgacc cggctcaccc gttccacctg
     3121 gaagccacag gccgattcgt gcgccagtca gcgttgacgc ggtggcaaac gggcgctata
     3181 atgatcgcgt gaagctgctt gccctggatg ttggaaacac caatatcgtc ctcgggattt
     3241 tcgaggacca gcgcctggtc gcatcgtggc ggctttcaac ggaccgtggt cgcaccgccg
     3301 acgagtacgg tgttctggcc acgctgatgc tggcgcaagc cggcatcacc cccaaagacg
     3361 tggggggaat tgccgtctgt agcgttgtgc cgccgctgga ccacaccctg cgcaccctgt
     3421 tgacgcgtac cttcgacgcc ccgatcctga ccgtggacca ctccaccgac acagggatcc
     3481 gcatctgcta cgagacaccc agcgacgttg gcccagatcg gatcgtcaac gcggccgcgg
     3541 cataccacca ggtggggggg ccgtgcgtcg tggtcgattt cggcacagcc actacctttg
     3601 acgtcgtcac cgcgaacgga gagtatcacg gcggggcgat tgccccaggc atcggcatct
     3661 cgatggaggc gctcttcgcc cgggcggcgc gcctcccgcg catcgatctg gcgcgcccgc
     3721 cacgcgtgat cgggaagact acgcgcgaga gcatgcagtc cggcttcgtc ttcgggttcg
     3781 ccggccaagc ggatgccatc atctcacgcc tctgcgccga gcttgggccg gagacgcggg
     3841 tggttgccac gggcggcctg gcagagctga tcgctcccga aacgcagagc atccgccacg
     3901 tccgcccgga gctgacgctg gagggcctgg agatcatctg gcgtaggcaa cagcgctgac
     3961 cgttttcccc ggcgaaggcc aggatgcaag agcagcgcgc ggccttcacc ggggcgcctt
     4021 tcctaaaagg cactggccgg gttatagcgg acgaaaagat tcgcgtg
//
LOCUS       DAOSCV010000348         4066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13928_length_4066_cov_2.634505, whole genome shotgun sequence.
ACCESSION   DAOSCV010000348 DAOSCV010000000
VERSION     DAOSCV010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13928_length_4066_cov_2.634505"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLY56_09450"
     CDS             complement(<1..124)
                     /locus_tag="PLY56_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOJ21747.1"
                     /translation="MLILAAIAEISGKRYGESEEVTKAFRIIADHMRCSTFIMGD"
     gene            complement(139..957)
                     /locus_tag="PLY56_09455"
     CDS             complement(139..957)
                     /locus_tag="PLY56_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21748.1"
                     /translation="MPSLVLLHTNDMHGRLAAEAAARIRAEKSRDPNTLLVDAGDALR
                     AGNLGVPLASETVLRHMNTAGYDLMAAGNRETHLWPWALRAKLRQAAFPVLCANVTGP
                     GVAAGDPVARTPAELRALARSEALYRPYVIVRIPNNLRVGIFGLTVPMVTREMAARHF
                     SSYLFRDPVETARDLVPLLARNADLVVGVLHLGLGRDCEIAREVPGIDLLVGGHTHDL
                     LPEPVVVGRTVVLQAGSYARYLGRVEVSVEAAGVSVRSHLLELREMEGGAQRSG"
     gene            complement(970..1503)
                     /locus_tag="PLY56_09460"
     CDS             complement(970..1503)
                     /locus_tag="PLY56_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOJ21749.1"
                     /translation="MMGGDPKRTRLLAMDVDGVLTDGTLYLTASGDEMKAFHVRDGMG
                     IALALCGGLEIAFVTGRSSDLVRRRATELGVRHVLEGIHDKRAALMELAERLGLAPAE
                     IAFVGDDLNDLPALSCCGLPVAVADAPQQVRAAAAWITEAPGGRGAIREVAERILEAQ
                     GKLDEAIARYLSGRTRQ"
     gene            complement(1770..2138)
                     /locus_tag="PLY56_09465"
     CDS             complement(1770..2138)
                     /locus_tag="PLY56_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015789384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOJ21750.1"
                     /translation="MAIKELLESIAERVGRAGVSMVFGEPHVVRTRTVIPVASVSFGF
                     GGGGGESAHDEGDQPASSGGGGGGGGKATPVAVIEVSDEGTHVLPIVDITRIRLAGFA
                     CLALGLWVLGRALPRIMRHR"
     gene            complement(2140..2889)
                     /locus_tag="PLY56_09470"
     CDS             complement(2140..2889)
                     /locus_tag="PLY56_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ21751.1"
                     /translation="MPRRVVLALVADIHCRAEIIEDLREGLMPVNDEADILVMCGDLC
                     NMGRVEEAKALADVLRGVRIPMVAVLGNHDYHSGYVNEVVAILEGVGVHLLQGTGHTI
                     NIDGTTVGFAGAKGFCGGYGTYCLTPFGEQGIKCFINETHDDANNLDACLQTLDTDYR
                     VVLLHYAPIKETLFGEPPELYPFLGSSILAEPVDKHGCDLVVHGHAHNGVEEGETAGG
                     ILVKNVAMPVIKRSYVLHELDASARARKGRQ"
     gene            complement(2889..3137)
                     /locus_tag="PLY56_09475"
     CDS             complement(2889..3137)
                     /locus_tag="PLY56_09475"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ21752.1"
                     /translation="MISDTTIARSAKTALANDPRVGAMDITVHSHRGRVQLVGYVTSP
                     EQIKAAEEVARSIPGVVEVINNLRLVRLTSRHETMEES"
     gene            complement(3429..>4066)
                     /locus_tag="PLY56_09480"
     CDS             complement(3429..>4066)
                     /locus_tag="PLY56_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ21753.1"
                     /translation="ASLAIEATAPSTEARDLDARLFRAINLKPGHKDALDPLLLSIMP
                     LGDRTAIAIPLGLYAWGRMEDNKQLRDAGVLTAAALASSALATEGLKGLVARKRPAER
                     FGDAHTRIVGRRAADGRSFPSGHTSNAFAWATVLADLYPKGSAAFYGTASAIAFGRVR
                     LGAHYPSDVLAGALIGYMAGRFVVSNRDFLMRREDPPTGGTAVGISRQFHF"
BASE COUNT          685 a         1341 c         1378 g          662 t
ORIGIN      
        1 gatcgcccat gatgaaggtg gagcagcgca tgtggtcggc gatgatgcgg aaggccttcg
       61 tgacctcctc ggattcgccg taacgcttgc cggaaatttc ggcgatggcc gcgaggatga
      121 gcaccagaag cagcgcggtc accccgatcg ttgtgcccct ccctccatct ctcgcagctc
      181 aagcagatgg gagcgcacgg agacgccggc cgcctcgacg ctcacctcca ccctccctag
      241 atagcgcgca tagcttcccg cctgaagcac gacggtccgg ccgaccacca ccggctcggg
      301 caggaggtcg tgcgtgtggc cacccacgag gagatcgatg ccggggacct cccgggcaat
      361 ctcgcaatcg cgccccaacc ccaggtgtag gacgcctacc acgagatcgg cgttcctggc
      421 gaggagcggc accagatcgc gggccgtctc caccggatcg cggaagagat agctgctgaa
      481 atgtcgtgcg gccatctctc gcgttaccat cggcacggtc agcccgaaga tcccgacgcg
      541 gaggttgttg gggatgcgca cgatcacata ggggcgatat agcgcctcgc ttctggccag
      601 agcgcgcagc tcggcgggcg tgcgggcgac gggatccccc gcggcgactc cgggtccggt
      661 gacgttggcg cagagcacgg gaaatgcggc ttgccggagc ttggcgcgca gtgcccacgg
      721 ccagaggtgc gtctcgcgat tgcccgcggc catcaggtcg tatccagcgg tgttcatgtg
      781 ccgcaggacc gtctcactcg cgagcggcac tccaagattg ccggcgcgaa gagcatcgcc
      841 ggcatccacc aagagcgtgt tcggatcgcg cgacttctcg gcacggatcc gcgcggcggc
      901 ctctgccgcg agccggccgt gcatgtcgtt ggtgtggagc aggacgagag agggcatgcg
      961 tgaactcgcc tactgtcgcg ttctgccgga gaggtagcgc gcgatcgcct catccagctt
     1021 gccctgcgcc tccaggatgc gctcggccac ttcacgaatg gcgccacgac cccctggcgc
     1081 ctcggtgatc caggccgcgg cggcgcgcac ctgctgaggg gcatccgcga cggcgaccgg
     1141 cagcccgcaa caggagagcg ccggaaggtc attcaggtcg tcgccgacga atgcgatctc
     1201 tgcgggcgct aggcccagcc gctccgccag ctccatcagg gcagcgcgct tgtcgtggat
     1261 tccctccagc acgtggcgga cgcccagctc tgtggcgcgc cggcggacga gatccgacga
     1321 gcgccccgtg acaaacgcga tctccaaccc gccgcagagg gccagcgcga tccccatccc
     1381 atcgcgcacg tggaacgcct tcatctcgtc gcccgaagcg gtcaggtaga gcgtgccgtc
     1441 ggtgagcacg ccatccacat ccatcgccag gaggcgggtt ctctttggat ctccacccat
     1501 cataggactt gctccaccgg gcggaccgcg gcggcgtccc ccgattggct ctcattataa
     1561 catacgtgcc tgggggcggc aatggcgcga gcagccactg agacaacgcc ctgggctaca
     1621 gagctgggat tccttatgtc ggaatctcag ggtacagagg cggtgcctcc ccgtgatgag
     1681 gggtggaagc atgtcgccga gagatggatg tggggagcca cgaggcgcgc gcccgccggg
     1741 cgagaaggcg gatgctctcg cgctcgcggc tagcggtggc gcatgattcg gggaagcgcg
     1801 cgccccagga cccagagacc cagggcaagg caggcgaatc ccgccaggcg aatccgcgtg
     1861 atatcgacga tggggaggac gtgggtgccc tcgtccgaga cctcgatgac agcgacggga
     1921 gtcgccttgc cgccgccacc gcccccaccg ccggagcttg cgggctgatc gccctcatca
     1981 tgcgcgctct ccccgccgcc gcccccgaag ccgaacgaga ccgaggcgac cgggatcacc
     2041 gtgcgcgtac gcaccacgtg cggctcgccg aaaaccatgg agacgcccgc gcgtccgacg
     2101 cgctcggcga tgctttcgag aagctctttg attgccacgt tactgtctcc ccttgcgcgc
     2161 tcgcgcgctg gcatcgagct cgtgaaggac ataggagcgc ttgatgacgg gcatcgccac
     2221 gttcttcacc aggatgccgc ccgccgtctc tccctcctcc acgccattgt gcgcatgccc
     2281 gtggaccacc aggtcgcacc catgcttatc gactggttcg gcgaggatcg aggaccccag
     2341 gaaggggtag agctctggtg gctcgccgaa gagcgtctcc ttgatggggg catagtgcaa
     2401 cagcacgacg cggtaatcgg tatcgagcgt ctgcaggcag gcatcgaggt tgttggcgtc
     2461 atcgtgcgtc tcgttgatga agcacttgat gccctgctcg ccaaagggcg tgaggcaata
     2521 ggtgccatat cccccgcaga agcccttggc gccggcgaag ccaaccgtcg tgccatcgat
     2581 gttaatggtg tgcccggtgc cttgcaggag atggacgccg acaccctcca ggattgcgac
     2641 cacctcgttg acatacccgg agtgatagtc gtggttgccg agaacggcga ccatcgggat
     2701 ccgtacgcca cgcagcacgt ccgcgagcgc cttcgcctct tccacgcggc ccatgttgca
     2761 caggtcgccg cacatgacga ggatatcggc ctcgtcgttg acaggcatca ggccctcgcg
     2821 tagatcctcg atgatctcgg cgcggcagtg gatatctgcg acgagtgcca gcaccacgcg
     2881 ccgaggcatc agctctcctc catcgtttcg tggcgagagg tcaggcgcac caggcggagg
     2941 ttgttgatca cttccaccac ccctggaatg ctgcgcgcca cctcctcggc cgccttgatc
     3001 tgctccgggc tggtgacgta tcccacgagt tgcacgcgcc cgcggtggct gtgtaccgtg
     3061 atatccatcg cccccacgcg gggatcattc gccagcgccg tcttggccga tctggcgatg
     3121 gtcgtatccg aaatcatggc ccctccatcg gtgcgtggcg ggtggccact tccgccctgc
     3181 gcgtacccgt tgcccgcaga gcgcgcgctc gtgccggaac tggaagtacg cgggatagcc
     3241 ctgggtccgg gcgaggttgc tcaccgcgat gggcggaacc tcgtcttcgc ccgcgtaacg
     3301 gtttacccat aggggcggct gagtaaacgc caacgcggcg ttcgatccgg tatggggccg
     3361 gcaggcaagg ggggacccgg gggtcgcatc aggatcggga tgccggggtg acggcctggc
     3421 gggtggatct agaaatggaa ctggcgtgat atgcccacgg cggtgcctcc cgtgggggga
     3481 tcctcccggc gcatcaggaa atcccggttg ctgacaacga agcggccggc catgtagcca
     3541 atcagggcgc cggcgaggac gtcgctcggg tagtgggcgc cgaggcgcac ccgcccgaac
     3601 gcgatggcgc tcgccgttcc atagaaggcc gcggagcctt tgggatagag atccgccagc
     3661 acggttgccc aggcgaatgc attggacgta tgccccgacg ggaaggagcg tccgtcagcc
     3721 gcgcgccggc caacgatgcg ggtgtgggca tctccaaagc gttcggctgg gcgcttgcgc
     3781 gccacgagcc ccttcagccc ctcggtggcg agggcactgg aagccagcgc ggccgcggtg
     3841 agaacacccg cgtcgcgcag ctgcttgttg tcctccatcc ggccccaggc atagaggccg
     3901 agggggatgg cgattgccgt gcggtccccg agcggcatga tggagagcaa gagggggtcg
     3961 agggcatctt tatgcccggg ctttagattg atggcgcgaa agagccgcgc gtcgaggtca
     4021 cgcgcttccg tagagggagc ggtggcctcg atggcgagcg atgcgg
//
LOCUS       DAOSCV010000349         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13959_length_4060_cov_3.612484, whole genome shotgun sequence.
ACCESSION   DAOSCV010000349 DAOSCV010000000
VERSION     DAOSCV010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13959_length_4060_cov_3.612484"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLY56_09485"
     CDS             complement(<1..742)
                     /locus_tag="PLY56_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21754.1"
                     /translation="MSPGVTFRCTCEHCGAIFFGPDRKATACIKCAKRHKIRTELPRQ
                     TADLDDDPFAAALISADPRPSRGSHRRAAAVPRGSSDAVVASRSAAPPGRTVTAHADA
                     SPMRGPRATELTEERRAQILSAYQEYEGRDETPLRRIHAEIAEKTNLARALVARVIAE
                     VRTPPAALTEEQRESIIARYREYVRTMARPATGRRATIAREMGLSRQQVVTVVREWGA
                     SQPTVTDLSREELFRIECAYCSALEAGEP"
     gene            1150..1226
                     /locus_tag="PLY56_09490"
     tRNA            1150..1226
                     /locus_tag="PLY56_09490"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1184..1186,aa:Glu,seq:ctc)
     gene            1897..>4060
                     /locus_tag="PLY56_09495"
     CDS             1897..>4060
                     /locus_tag="PLY56_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ21755.1"
                     /translation="MLAEVPMNNSRKFPEQWPVWLHCGVVILTVAVAASIRVIGWPVL
                     QTEIPWLLFVPGVMLVAWVGGLIPGLAATVLSALTVDYLLLEPLRSLGVHNYSQAVGY
                     GLFLSTGILISLWVDHARAVQARLRETAHGLVLANEALREGEARLQAIVDGLNEGIVI
                     ADLEGHLVGWNRAALAMHQIAHDPIGPRSLEELESFYELSTLDGTVLPPEQWPLARVL
                     RGERLQGWDLRIRRLDTDWERFFSYGGTLAYGEDGRPLIAVVTINDITERKHAEQALR
                     RANEEWERTFDSVPDLIAILDVNYQVVRTNRAMAERLGVTPGATIGTPCYRCVHGAKQ
                     PPEGCPHALTLKDGKQHVAEVHEEHLGGHFLVSTTPLHDEQGRMIGSVHVARDITERK
                     RSEERTRFLSEASSILASSLDYDETLQRVAELALPALADVCVIDLVEEDARVRRVTAR
                     ASDPAQQALVDRLCTDGSSGPCPASISEVIASGKPVFVPEIDAAALAAAARTGRCLEI
                     LRQLSLCSFLAVPLVARGRVLGAFSLMMTTSGRRCCEDDLQIAQELGRRAALAIDNAQ
                     LYAGERKARAAAEEADRAKDQFVALVSHELRNPLNAIQAGVGLLQHLGTSDPRAARTL
                     EIIGRNVKLQARLVNDLLDLSRIQREKLQFQHAPVEIARMVTAAVQASASEASEAGLS
                     LTSTVIPDLWVHGDFDRLQQVVMNLLSNAIKFTPSGGEI"
BASE COUNT          706 a         1335 c         1271 g          748 t
ORIGIN      
        1 acggctctcc cgcctccagc gcggagcagt aggcgcactc aatccggaag agctcttccc
       61 ggctcagatc ggtgaccgta ggctggctgg caccccactc tcggaccacg gtgaccacct
      121 gctggcggct gagccccatc tcccgggcaa tcgtggcgcg gcgcccggtg gccggacgcg
      181 ccatagtccg cacatactca cggtaacggg caataatcga ctcgcgctgc tcctcggtga
      241 gcgcggcggg tggggtgcgc acctcggcaa tcacccgtgc gacgagtgcc cgagcgagat
      301 tcgtcttctc agcaatctcc gcgtggatac ggcgtagggg agtctcatcg cgcccctcat
      361 actcctgata ggcgctcaag atctgggcac gccgctcctc ggtcaattcg gttgcccttg
      421 ggccccgcat tgggctggca tcggcatggg cggtaaccgt gcggccagga ggcgccgccg
      481 agcgcgaggc cacgacggca tcactggagc cgcgtggcac ggccgcggcg cggcggtggg
      541 aaccacgcga tgggcgagga tccgcgctga tcaacgccgc ggcaaagggg tcatcatcga
      601 gatcggccgt ctgccgaggc agctccgtgc gaatcttgtg gcgcttggcg catttgatac
      661 aggcggtcgc ctttcggtcc ggcccgaaga agattgcccc acagtgctcg cacgtacagc
      721 ggaaagttac gcctggactc atcagcttct ccatcggtgc gactgcggcg cccgatacgc
      781 cgcggtgcgg cgccggcacc acactacacg cacggggaat cgcgcactcc atgcctcgct
      841 tttgcctgcc gccagcgggc aggcaagcca gacataccca gtcgtctctc tatccaccgg
      901 ccccgggcgc cacgccctcc ggcaataaga gggcagccaa caccacggcg acggggcaca
      961 acaggtcggt ttgcctatta tagcacgatc ccacaccatt tggcaaagtc cctcacaacc
     1021 tgcgccggcg gtcgtgaaca aaccccaaaa tgaagcgcaa attcggggtt gacaggcaag
     1081 ccgcctcatg ctataatctg ggtgacgttg cgggagggaa gtttcttcaa ttcccgcgat
     1141 agagctggtg gcccggtcgt ctaattggtt aggacaccgc cctctcaagg cggagcttgc
     1201 gggttcgatc cccgtccggg ctaccatttt ttatgtacag tatccgctgg aatacaggtt
     1261 ccatccgccc cagccttctg tcccatcagt atccgaatgg atgatcctgc tcactcgggc
     1321 tcgttccaca gccacgcctt ttccaccgac tgcccgatgg atacggcacc cgagtttgct
     1381 ctctgtccac gctccttttc aacctactag ccgttcgatt cgggtttgtt ctcgtgccta
     1441 gcgattgccc tctccggcat cggccgggtg cggaaggcgg aagagctctt tccttgcctc
     1501 cgggagggcg ttcgattctc tctccagacc ctgggcaact gctgtgcgca gcgcgcatgc
     1561 cttcgcaggc cgccagaacg ggtgggccag gaggaacgtg cctcacgaaa ccgcgggggc
     1621 gccggggcac ctggcatcca agactgccct ccaaagcaaa gcacttcctc cggaacccgg
     1681 atggggaatc tgccgtatac atggttgagc acgggtgcct ggaagccgct ggtcggtgcc
     1741 acgcgatggc agtggctgcg agtaccctcg cacttgccga aattctttgt actttcccga
     1801 atgagatgct atcgaaacgg gaatactgat tcatgggggc tcttctctcg ccgccaactt
     1861 agcgccctcg gatcaccttc catggaaaag gagcccatgc ttgctgaggt gccgatgaac
     1921 aactccagga agtttccgga gcagtggccg gtctggttgc attgtggcgt cgtgattctg
     1981 accgtcgctg ttgcggcctc catccgggtg atcggctggc ctgtcctaca gaccgaaatc
     2041 ccctggctcc tcttcgtccc cggggtgatg ctggtcgcct gggtgggggg tctgatcccc
     2101 gggcttgctg caaccgtctt gtccgcgctc acggttgatt acctccttct ggaaccgctg
     2161 cgttcactgg gagtccacaa ttacagccag gcggtgggct atggcctctt cctctccacc
     2221 ggcatcctga tcagcttgtg ggtagatcat gcgcgcgccg ttcaggcacg cctgcgggag
     2281 acagcacacg ggttggtgct ggccaacgaa gccttgcgcg aaggtgaggc ccggctgcaa
     2341 gccattgtgg atggcctcaa cgaggggatc gtgatcgccg atctcgaagg ccacctggtt
     2401 ggctggaacc gtgcagccct cgcgatgcat cagattgccc acgaccccat tggccctcgc
     2461 agcctggagg agctcgaaag cttctatgaa ctctccaccc tggatggcac cgtattgccc
     2521 ccggagcagt ggccgctcgc ccgcgttctg cgcggcgagc gcctgcaggg ttgggactta
     2581 cgcatccggc gcctcgatac cgactgggag cgcttcttca gctatggagg aaccctcgcc
     2641 tatggggaag atggccggcc gctcattgca gtggtcacta tcaacgatat caccgagcgc
     2701 aagcatgctg aacaagcgct gaggcgcgcc aatgaggagt gggagcgcac tttcgatagc
     2761 gtcccggatc tgatcgcgat cctcgacgtg aactaccagg tggtgcgaac caaccgggcg
     2821 atggcagaac gcctgggggt cacccctggc gcgactatcg gcacgccctg ctatcggtgt
     2881 gtgcatggcg ccaagcaacc accggaaggt tgccctcatg cgctgacgct gaaggacggg
     2941 aagcagcacg tggccgaggt gcacgaagag cacctcggcg gccatttcct ggtgagcacc
     3001 accccgctgc atgacgagca gggccgcatg atcggctcgg tacacgtcgc tcgcgacatc
     3061 actgagcgca aacggagcga ggagcgcacc cgcttcctct cggaggctag cagtatcctg
     3121 gcatcctcgc tggactacga cgagacgctg cagcgcgtgg ccgaactggc gctgccggca
     3181 ctcgccgatg tgtgcgtcat cgacctcgtg gaagaagacg cgcgcgtccg gcgggtcacg
     3241 gcacgggcca gcgacccggc ccagcaggcg ctcgtcgaca ggctgtgtac cgatggctct
     3301 agcgggccct gcccggcgag catctctgag gtgatcgcct cgggaaagcc tgtttttgtt
     3361 ccggagatcg atgcggccgc gctggcggcg gccgcccgga ccgggcggtg cctcgagatc
     3421 ctgcggcagc tgtccctctg ctctttcctt gccgtgccgc tggtcgcgcg cggccgcgtg
     3481 ctcggggcgt tcagcctgat gatgacgaca tcgggccggc gctgctgcga ggatgacctg
     3541 caaatcgcac aggagctggg ccggcgcgcg gcgctggcca tcgacaacgc gcagttgtac
     3601 gccggcgagc gcaaagcccg tgccgccgcg gaggaggccg accgcgccaa ggatcagttc
     3661 gtcgcccttg tctcgcacga actgcgcaac ccgctcaacg ccatccaggc cggcgtcggt
     3721 ctcctgcagc acttgggcac gagcgacccg cgcgcagccc gcaccctcga gatcattggg
     3781 cgcaacgtta aactccaggc gcgcttggtg aatgatctgc tggatctgtc gcgcattcag
     3841 cgagagaaac tccagtttca gcacgcccca gtcgagatcg cccggatggt cacggcggca
     3901 gtgcaggcct ccgccagcga agcctcagag gcgggcctga gcctcacatc caccgtgatc
     3961 cccgatctgt gggttcatgg cgatttcgac cgcttgcaac aggtggtgat gaacctcctc
     4021 tccaacgcga tcaagttcac cccatccggg ggggaaatcc
//
LOCUS       DAOSCV010000350         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_13981_length_4054_cov_3.254564, whole genome shotgun sequence.
ACCESSION   DAOSCV010000350 DAOSCV010000000
VERSION     DAOSCV010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13981_length_4054_cov_3.254564"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLY56_09500"
     CDS             <1..194
                     /locus_tag="PLY56_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21756.1"
                     /translation="AVLLGVAALAWHRREGRWPRWGALAPLAARIAANPRTRRRTLWM
                     AIQLLLLDLLAPLALIALK"
     gene            complement(235..987)
                     /locus_tag="PLY56_09505"
     CDS             complement(235..987)
                     /locus_tag="PLY56_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ21757.1"
                     /translation="MKPIAIQLYSVREAAAKDFPAVLKRIADMGYKGVEFAGLHGMAP
                     AEVRKVIDDLGLQVSSAHLPLPTAENCAELVDQCRTLGITRLVAGFGPDQLKTVELCK
                     QAAEKVATAVSLLEGTGIALGLHNHWWEFETVEGRFPEDILLEASPSVFAELDVYWAQ
                     VGCGDAVKAVADRKQRVLLLHAKDGMIEPKQPMTAVGSGKLDFPKILAATDPNVLEWV
                     IVELDSCATDMMEAVERSYRYLVDNGLAVGNK"
     gene            1427..2140
                     /locus_tag="PLY56_09510"
     CDS             1427..2140
                     /locus_tag="PLY56_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21758.1"
                     /translation="MLYRQRGSVTGFTLIELLVVIAIIAILAAILFPVFSRARENSRK
                     SACQSNMKQIAMAVLQYVQDYDETVPPHAHRMIDGQTLEDLLAGRPQGPWCVYIQPYV
                     KSWNVFICGSDKERSWNVETSAPANVSVKDPPNSYGWNYNFVQGGPYRMAVIEEPASI
                     VMLCEQSGRLPYGRWWNSAARMGSGSHYIWRYDRHSDGANYAFWDGHVKWMPGNLVPK
                     SGTAAVWPTQNFRFQPVWP"
     gene            2272..3993
                     /locus_tag="PLY56_09515"
     CDS             2272..3993
                     /locus_tag="PLY56_09515"
                     /inference="COORDINATES: protein motif:HMM:NF018606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HOJ21759.1"
                     /translation="MQWMPRIEARIVGLLLAGMLVCAGTPAAKGASGEASAIPAVDEA
                     RLNTVRIFADRVLQYGRDRYREKPTPLFVDGLNVDTLEPVRWVYKGERWIPSNLASQQ
                     NLFRTLVGLSNLTGDPRYRKAAEEAIAYHFEHLRSPCGLLPWGGHRWVDLATGNFVGE
                     GGRPHEFKLNLPFYELMWEVNPSATEQFIKAFWNAHILDWGKLDMNRHGAYGKPMGAL
                     WDNTFEDPEPFFEGDGLTFLNAGTDLIYAGVLLYKFTGDQGALTWAKRLAYQYVKARH
                     PKTGLGVVQYSKPVRRQQPPATGPLPEGSNYGDRAENQFGAEFGEVAREGYMIRSPGT
                     LYEGNAIAQLQLAELLGKEGSDFLKWTVDGLRAYARYGYDPKTNRTRPLWADGTDLTG
                     YVIKRDGYYGKAGTVFRASTASLKLFWSYALAHRLSGDRVLWETARSIARGHGLGDLG
                     SAPGRDVRVNLDTQNAHPMALFGLLEVWRMADVPAYRALARRIADNMVRQRFHRGFFL
                     PSADRVNANFDTLEPLAILSLEAMLRGTPEAVPRYTGGQGFIHGPHDGLGRTYDSTAI
                     WSVRRKR"
BASE COUNT          707 a         1349 c         1302 g          696 t
ORIGIN      
        1 tagccgtcct cctcggggtc gctgcgctgg catggcaccg gcgcgagggg cgctggcctc
       61 gatggggcgc gctcgctccc ctggctgcgc gaatcgccgc caacccgcgc acccgcaggc
      121 gcaccctctg gatggcgatc cagctcctcc tcctcgacct cctggccccg ttggccctca
      181 tcgcgctcaa gtagcaggcc ggggggatcg agatcccccc gtggccactg aaccctactt
      241 gttccccacc gcaaggccgt tatcgaccag gtagcggtag ctgcgctcca cggcctccat
      301 catatccgtc gcgcacgagt cgagctctac gatcacccac tcgaggacgt tcggatcggt
      361 tgcggcgagg atcttcggaa agtcgagctt cccggagccg accgccgtca tcggctgctt
      421 cggctcgatc atgccgtcct tggcgtggag gagcaagacg cgctgcttgc ggtccgccac
      481 ggctttgacc gcgtcgccgc agcccacctg ggcccagtag acatccagct cggcgaagac
      541 cgagggcgac gcctccagaa gaatatcctc cgggaagcgg ccctccacgg tctcgaactc
      601 ccaccagtgg ttatgcaggc ccagggcgat gcctgtgccc tcgaggaggg aaacggcagt
      661 cgcaaccttc tctgccgcct gcttgcacag ctccaccgtc ttgagctggt cgggcccgaa
      721 accggcgacg agccgcgtga tcccaagggt gcggcactgg tcgacgagct ccgcgcagtt
      781 ctccgccgtg ggcagcggca ggtgggcgct gctcacctgc agcccgaggt catcaatgac
      841 cttccgcacc tcggccggtg ccatcccatg caggccggcg aactcgacac ccttgtagcc
      901 catgtcggca attctcttca ggaccgcggg gaagtccttc gccgcggctt cgcggacgga
      961 atagagctga atagcgatcg gcttcatgct cgtggctcct tcgcaatgga tcagatgagg
     1021 agacgaggct tcgtccctcg ggcggtagtg ttcggtgccc ggccggggag gtcctgcgcc
     1081 cggcgtccgc attaccccgg cccatgatgg cgtcgatacc acggatgaag aggacggaca
     1141 cggacgagcg tctgctttcc taacccccga cccctcgcgg accctcgggc gccggccctt
     1201 ctctgagcaa cagtgttttt aggctccacc ctcttgacaa ctatggtacg gttatgatac
     1261 taagagggcc aaggtatcgg gcgagcgccc gcgccagaga aagggggcgg gatggcgagc
     1321 gtctgcgagc cagcgccgaa gaccggccag tcgcggccgg tctcgcgcgg gcagagcctc
     1381 tcgagaggcg ccgcgcccga cacccgacgg gaaggaggtt tcggccgtgc tgtacaggca
     1441 gagaggttcc gtaacaggat ttacgctcat cgagctgttg gtggtcatcg cgattatcgc
     1501 cattctggcg gcgattctgt tcccggtctt ctcgcgcgcg agggagaaca gccgcaagtc
     1561 cgcctgccag agtaacatga agcagatcgc catggccgtg ttgcagtatg tccaggacta
     1621 cgacgaaacc gtgcccccgc acgcgcaccg gatgatcgat gggcagacgc tggaggatct
     1681 gctcgcaggc cggccccaag gcccctggtg cgtctacatt cagccctacg tcaagagctg
     1741 gaacgtgttc atctgtggca gtgacaagga acggagctgg aacgtagaga cctctgctcc
     1801 cgccaacgtt tctgtcaagg atccgccgaa cagctacggg tggaactaca acttcgtcca
     1861 gggcgggccg taccggatgg ctgttatcga ggagccagcg tcgatcgtga tgctgtgcga
     1921 acagagcggt cggctgccct acgggcggtg gtggaacagt gcggcgcgta tgggctctgg
     1981 ctcgcactac atctggcgtt acgaccggca ctcggatggc gccaactacg ccttctggga
     2041 cggccatgtc aagtggatgc ccggcaatct ggtgcccaaa tcgggcacgg ccgcggtctg
     2101 gccgactcaa aacttccgct tccagccggt gtggccctga gccccagtcc ggcctttcgt
     2161 agcggggtgc gctgctggcg cgtcccaccg atctgcccgg gcgggcgcgc gatggcagat
     2221 cgggtcgcgc gcccagaccg cctctcgttt cacaggcgcg aggaggggac catgcagtgg
     2281 atgccgagga tcgaagcacg aatcgtcggg ctgctacttg ctggcatgct ggtgtgcgcc
     2341 ggaacgccgg ccgcgaaggg cgcatcggga gaagcatccg ccataccggc ggtggatgaa
     2401 gcccgcctca ataccgttcg gatctttgcc gaccgggtgc tgcagtatgg ccgtgatcgc
     2461 taccgggaga aaccgactcc cctgttcgtg gatggcctca acgtggatac gttggagccg
     2521 gtgcgctggg tctacaaggg cgagcgatgg attccatcca acctggccag ccagcagaac
     2581 ctgtttcgca ccctggtggg cctgagcaac ctcaccggcg acccgcgcta ccggaaggcg
     2641 gcggaagaag ccatcgcgta tcacttcgaa cacctgcgca gcccgtgcgg cttgctcccc
     2701 tggggtggcc accgctgggt ggatctcgcc accggcaact tcgtgggcga aggaggccgc
     2761 ccgcacgaat tcaagctcaa cttgcccttc tacgagctga tgtgggaggt gaacccctcc
     2821 gccacggagc agttcatcaa agcattctgg aacgcccata tcctcgactg gggcaagctc
     2881 gatatgaacc gccacggcgc ctatggcaag ccgatgggcg ccctgtggga caacaccttc
     2941 gaggacccag aaccgttctt cgaaggcgac ggcctcactt tcctcaatgc cggcaccgac
     3001 ttgatctatg ccggtgtcct cctttacaag ttcacgggcg accagggcgc gctcacctgg
     3061 gcgaagcgcc tggcctacca gtacgtgaag gcacgccatc ccaagaccgg cctgggcgtg
     3121 gtccagtaca gcaagccggt ccggcggcag caacccccgg cgacgggccc cctacccgaa
     3181 gggagcaact acggagaccg cgcggagaac cagttcggcg ccgagtttgg cgaggtcgcc
     3241 cgcgagggct acatgatccg ctcgcccggc acgctctacg agggtaacgc catcgctcaa
     3301 ctccagctcg ccgagctgtt aggaaaggaa ggttccgact tcctcaaatg gacggtggat
     3361 ggcctgcgcg cctacgctcg ctatgggtat gaccccaaga ccaatcgcac ccggccgctc
     3421 tgggccgacg gcaccgatct caccggctac gtgatcaagc gcgacggcta ctatggcaaa
     3481 gcgggcaccg tcttccgggc ctccacggcg agcctcaagc tgttctggtc ctacgcgctt
     3541 gcccaccgtc tcagcggcga ccgcgtgctc tgggagacag cgcgcagcat cgcacggggc
     3601 catggcctgg gcgacctggg atcggcccca ggccgggatg tccgtgtcaa cctggacacg
     3661 caaaatgcgc atccgatggc gctcttcggt ctactggaag tgtggcgcat ggcggatgtg
     3721 cctgcctatc gcgcgctcgc ccgacgcatc gccgacaaca tggtgaggca gcggttccat
     3781 cggggcttct tcttgcccag cgccgaccgt gtgaacgcca atttcgacac cctggaaccg
     3841 ttggcgattc tctcgctcga agcgatgttg cgcggcacgc ccgaggcggt gcctcgttac
     3901 accggcggtc agggcttcat ccacggccca catgatggcc tgggccgcac ctacgactcc
     3961 actgccatct ggtcggtgcg gcgaaagcgc tagcgcggga gcctcgctac cgcggttgat
     4021 agcgacggac gcggatgggt aggaggcgga tcgc
//
LOCUS       DAOSCV010000351         4025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14107_length_4025_cov_3.302267, whole genome shotgun sequence.
ACCESSION   DAOSCV010000351 DAOSCV010000000
VERSION     DAOSCV010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14107_length_4025_cov_3.302267"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PLY56_09520"
     CDS             complement(<1..931)
                     /locus_tag="PLY56_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOJ21760.1"
                     /translation="MSINPTRKRMVKLGFCPIGKFVFSHEDALRQKARLEEKLRLWGV
                     EYASLDGILKDGIVRSQADVDAVVDHFRREEIDALFLPHCNFGTEGAAGMIAKKLERP
                     VLLWGPRDEAPLPDGTRLRDTLCGLFASSKVLHKLGVPFTYIENCGVDDPTFERGFRD
                     FLGAASVAGLFRRGPRIGHIGQRIDFFWSTIINESELLERFRVEVLPLDLVEFIRTAK
                     DRASRARAAYEREARELRQTAIIEHLEDGALINILAARDQMLALAEEHGLDALAVQDF
                     TSLVDEMGAYCFYANSAVGDRYPVGCESDIHGAL"
     gene            1242..2036
                     /locus_tag="PLY56_09525"
     CDS             1242..2036
                     /locus_tag="PLY56_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21761.1"
                     /translation="MEALSPSPWTENQALEWDGLLLSRGTLLGWMGAVIPGVVSDPYD
                     PTPVPYLTAPPTNPRLNQERDLLPQTRQALHSALDGIGIRWFITQGISQDPLSAGTHR
                     PDGRSLSGSYYSAVADISIAGLTTKVPRHRCTPSQARAGKCLTPAQEQQFHRVLRRLR
                     DAGFAAWYRHFYDRRRGIWENEIHAIDPAAPFIKASLRRQMDDYLRGRDGLVGHTTRD
                     AAGPIYPNRAAFCRRAALSASIKGYRPFRSDCIRYQSATKSRYCGR"
     gene            2345..2569
                     /locus_tag="PLY56_09530"
     CDS             2345..2569
                     /locus_tag="PLY56_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ21762.1"
                     /translation="MARKVPEVLTAKQAKDFLQISDSTLRRYIRKGKLKAYRLAGERD
                     LRFRREDLLALLEPANGVVEKEPDQEEPLT"
     gene            2673..3992
                     /gene="mtaB"
                     /locus_tag="PLY56_09535"
     CDS             2673..3992
                     /gene="mtaB"
                     /locus_tag="PLY56_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOJ21763.1"
                     /translation="MDYRVALYTMGCKTNQYDSNRLAAEFEAAGFQLVPFDAAADIYV
                     INTCTVTAEADREARNASRRALARNPEALVIATGCYPQVNPEPMAEIPGVRLVGGNDL
                     KRDLVARVRALLAEEGAPAGSTVESEPAPSRAALTGVSAIGRTRHVVKIQEGCNHRCA
                     YCVVPHARGPARSRPVAEVVEEVRRVADAGFREVVLTGTCIGSYGKDFRPRVKLAECV
                     AAVAAVPGLKRVRISSIEPWDVDDELIALVAGDPVVCAHFHLPLQSGDDGLRRRMRRA
                     GTSAQFADLVARIRERLPDAGITTDVIVGFPGETDAEFEVTRDFCKRMQFSRMHIFRY
                     SPRPFTHAATLPDRVPRAIANARSRVLIRMGEEMSQAFACRWVGRSVSVLVEGQNDPE
                     DRFEGLTESYVRVLFTSERALARGELVNVEVCEAREGCLYGRVSGNE"
BASE COUNT          650 a         1273 c         1416 g          686 t
ORIGIN      
        1 tcagcgcgcc gtggatgtcc gactcgcacc caaccgggta gcggtcgccc accgcgctgt
       61 tggcatagaa gcagtaggcg cccatctcat ccacgagcga ggtgaagtcc tggactgcca
      121 gggcatccag gccgtgctcc tcggccaggg cgagcatctg gtcgcgcgca gcgaggatgt
      181 tgatcagggc gccatcttcc agatgctcga tgatcgccgt ctggcgcagc tcccgcgctt
      241 cccgctcgta ggccgcgcgg gcgcgcgaag ctcgatcctt cgcagtgcgg atgaactcca
      301 ccaggtccag ggggaggacc tcgacccgaa accgctccag aagctcgctc tcgttgatga
      361 tggtggacca gaagaagtcg atccgctggc cgatgtgccc gatgcggggc ccccggcgga
      421 agaggcccgc cacacttgcc gcgccgagga agtcgcggaa gccgcgctcg aaagtgggat
      481 cgtccacgcc gcagttctcg atgtaggtga acggcacgcc gagcttgtgg aggaccttgc
      541 tcgatgcgaa gaggccgcag agcgtgtcgc gcaggcgcgt gccgtccggc aggggtgcct
      601 cgtcgcgcgg gccccacagg agcaccgggc gctccagttt cttcgcgatc atcccggcgg
      661 cgccctccgt gccgaagttg cagtggggga ggaagagggc gtcgatctcc tcgcgccgga
      721 aatggtccac cacggcgtcg acgtccgcct gcgagcggac gatcccgtcc ttcaggatgc
      781 catcgagcga cgcatactcc acgccccaga ggcgcagctt ctcctccagc cgggctttct
      841 gccgcagggc atcctcgtgg ctgaagacga acttgccgat ggggcagaag cccagtttta
      901 ccattcgctt gcgggtggga ttgatgctca tgccggtgcc ctccggcccc attatagcat
      961 gcggccccgc gcagggcaac gccgctgtgc gccgcgcgag cacgaggaat cgctccttcc
     1021 ggcgcgccgg ggaaggcagg aaacttgcct gttaggccag catggcgggc aatctgaaac
     1081 actgcgagcg cctggcctcc gcgcggggcg gaggaccggg gcgacatcac ccgggtggat
     1141 gggcagtcgg ggccgctgcg gtcgcggtga ttgccattgc tgccaccgcc gcattgctgc
     1201 cgctcccatc gggcgtggta tgggcggagc gaggcccggg catggaggcg ctttcgccat
     1261 cgccgtggac cgagaatcag gcgctagagt gggatgggct gctgttatcc aggggtaccc
     1321 tgctcggctg gatgggagcc gtgatcccgg gggtggtatc cgatccgtat gaccccactc
     1381 ccgtgcccta tctgacggcg ccaccgacca accccaggct caaccaggag cgcgatctcc
     1441 tgccacagac gcggcaggcg ttgcacagcg ccctggatgg gatcgggatc cgttggttta
     1501 tcacgcaggg gatcagccag gatcccctga gcgccggcac ccatcgcccg gatggcaggt
     1561 ccctatcggg cagctactac agcgcggtag ccgatatcag catcgccgga ctcaccacga
     1621 aggtgccccg ccatcgctgc accccgagcc aggcgagggc aggcaagtgt ctgacgccgg
     1681 cccaggagca gcagtttcac cgggttctgc ggcgattgcg cgacgcgggc ttcgccgcct
     1741 ggtaccgcca cttctacgac cggcgccggg gtatctggga aaacgaaatc catgccatcg
     1801 acccggcggc gcccttcatc aaggcatcgt tgcgaaggca gatggatgac tacctccggg
     1861 ggcgcgatgg cctggtgggc cacacgacac gcgatgccgc cggtccgatc tatccgaatc
     1921 gggcggcatt ctgccgtcgc gctgctctga gcgccagcat caagggctat cgccccttcc
     1981 gcagcgattg catccggtat cagtccgcca cgaaatcccg ctactgcggc cggtgaagac
     2041 gcgaacgtct ccgcgcgcct cgccgtcact tctcctggcg gggcgcgatg ggcaagcctc
     2101 cgcgcgcccg catccctgcc aagggggcgc ccgccgggcg aggccgtgtg gctcagtctt
     2161 tcggccgcaa ggcccggggt cgcctggctc ggggtgagag cgtgttttga gtaacaaagg
     2221 caagtgttga cgcggtacgg aaggctatga tacaatcctg ggactgagag gatctgcctc
     2281 tccggagatc gtgtgtggag tagccgccgg cgctggccgg tcttggcact aggaggttcg
     2341 ttgtatggcc cgcaaagtac ccgaagttct gaccgccaag caggcgaagg acttcttgca
     2401 gatcagtgat agcacgctgc gccgttacat ccgtaagggc aagctgaagg cgtaccgtct
     2461 tgcgggcgag cgcgatcttc gctttcgccg ggaggacctt ctggcactcc tcgagccggc
     2521 gaatggggtg gtggagaagg agcccgacca ggaggagccg ctgacgtagc ccgagggtgt
     2581 tgtgtcgcgg cgcgggtggg ccagaccacg gccggtcctg cgcctttttc tttgtccggt
     2641 gagtgatgag tgagaggatt gccagcggtc gcatggatta ccgggtcgcg ctctacacga
     2701 tggggtgcaa gacgaatcag tacgacagca accggcttgc tgccgagttc gaggcggcgg
     2761 gattccagct cgttcccttt gatgcagctg cggatatcta tgtgatcaac acctgcaccg
     2821 tgaccgcgga ggcggatcgc gaggcgagaa acgcctcgcg ccgggcgctg gcgcgcaacc
     2881 ccgaggcgct cgtgattgcc accggctgct acccgcaggt caaccccgag ccgatggcgg
     2941 agattcctgg cgtgcgtctg gtcggcggca acgatctcaa gagagatctg gtcgcgcgtg
     3001 tgcgcgcgct gcttgcggag gagggcgcgc cggcaggttc cacggtggag tcggaacccg
     3061 ctccctccag ggcggcgctc accggggtga gcgctatcgg gcggacgcgc cacgtggtga
     3121 agattcagga gggctgcaac caccgctgcg cctactgcgt ggtgccccac gcgcgcggcc
     3181 cggcgcgcag tcgccccgtg gccgaggtgg tcgaggaggt gcgccgcgtc gcggatgccg
     3241 gctttcgcga ggtggtgctc actggcactt gcatcggctc ctacgggaag gacttccggc
     3301 cgcgagtgaa gctggccgaa tgtgtcgctg cggtggccgc ggttcccgga ttgaagcgtg
     3361 tgcgcatcag ctccatcgag ccgtgggacg tggacgacga gctgatcgcc ctggtggcag
     3421 gcgatccggt ggtttgcgcg cacttccacc tcccgctcca gagtggggat gatggcctgc
     3481 gccggcgaat gcgtcgggcg ggaacgagcg cgcagtttgc cgacctcgtc gcccgaatcc
     3541 gcgagcggtt gccagacgcg gggatcacga ctgacgtgat cgtcggcttc ccgggggaga
     3601 cggatgccga gttcgaggtg acgcgcgatt tctgcaagcg gatgcagttt agccgaatgc
     3661 atatctttcg gtattcgccc cggccgttca cccacgcggc gacgctgccc gaccgtgttc
     3721 cccgtgccat cgccaacgcg cgcagccgcg tgctgatccg gatgggcgag gagatgagcc
     3781 aggcctttgc ctgccgctgg gtggggcgca gcgtctccgt cctggtcgag gggcagaatg
     3841 accccgagga ccggttcgag ggcctcaccg agagctatgt gcgagtgctc ttcacgagcg
     3901 agcgcgcgct ggccaggggc gaattggtaa acgtcgaggt gtgcgaggca cgtgaggggt
     3961 gcctttacgg gagagtttca ggtaatgagt gaatgcatct tctgccggat tgccgcgggg
     4021 agtct
//
LOCUS       DAOSCV010000352         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14150_length_4014_cov_3.239454, whole genome shotgun sequence.
ACCESSION   DAOSCV010000352 DAOSCV010000000
VERSION     DAOSCV010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_4014_cov_3.239454"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLY56_09540"
     CDS             complement(<1..649)
                     /locus_tag="PLY56_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21764.1"
                     /translation="MARDLTRSELLRPFWCGSRFTWVAALTACALLSAMTQASARERQ
                     LAGIRLGRPANQVTELFGDPTRTFTGVPTGGQAGMPGAEGMGVPGMAPGGPAMPGMEA
                     GMAGDPMMGMAPGGMGAGAGIAVPPRQKYEGKIAAMKPEEFVGRLLAAPAAAMPVGGV
                     PGMEMAPGAMPGGVTTLFPWLSFPGLSVPGATKATRAIGPRGVTDFLEIEEPGGDV"
     gene            complement(767..2296)
                     /locus_tag="PLY56_09545"
     CDS             complement(767..2296)
                     /locus_tag="PLY56_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ21765.1"
                     /translation="MALLGVAPAHARLAERLERGETITVVALGDSLAAGFELSDPKRD
                     AYPSIFASVLQRRYPRARIRLIPAGIPGDTARGGVRRIPSDVLSHNPDLVTIQFGGND
                     HGRGRAPAEFERDLEEIVRLIREQPGTAALLATPPIVSPDADTPMVRAVKEIGKRAGI
                     PVADFDGAIRARGTGPRGPFPFGRHPDARLHAVMARSLYEAVETLLPCQRHVRVRIQE
                     GARYFPRADSAPVEVLVEAPGPDPVTVVLECDGVEQRQVVDPGARTMVPVSFQVSLAK
                     EGVPARRRLFAWARTGDAFDYDLRWLTATPVARAGQDGLVIDGRGLVLGRDGWRGPDD
                     LSGRATLIREGDFLHLTVTVEDDRVVRNRRIPNAFDSDCVELFLDCRPAGTQARPYWE
                     QGVVGLFIQPGTSHAEKASWKPLEPLPDAWQALEAHSALMPGGYRIDLRIPLSLLGMP
                     GARYVGFDLALDDADGPRGRRMQMVWAGGADDFLDPSLFGAVDLEPEPWAEPQMRVSV
                     W"
     gene            2787..3527
                     /locus_tag="PLY56_09550"
     CDS             2787..3527
                     /locus_tag="PLY56_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21766.1"
                     /translation="MASQEVLWPIYQIQKLDAQIALLQRARGALDDGAALAAEVTQHR
                     GDILSLQESLRQVDRAIRERELELASLEEKLQKVEAILYSGKVTNPKELRGYEGERES
                     LRRRRERLEEETLIRMEEKDSLNERLAAAQKVEKEAYARWQAHVAKFRADVAALDAKY
                     AKLVAERDEVRARADSELLRRYDQLARQRKQLVGLVEQNACGACGMLLPARLVKEYQV
                     NPDAELTCPTCSAHLLWRLPGADSARPA"
     gene            3638..>4014
                     /locus_tag="PLY56_09555"
     CDS             3638..>4014
                     /locus_tag="PLY56_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21767.1"
                     /translation="MRASGLWWVVVVALLGGGCDSLSRMKLAGGADIAADSQDARVTH
                     PQRSLATLLERQGLPQTLLCSGTTWRAVGLGGFRNEQRLAPVKPGIAGYTLLHERGAR
                     RDEPPRLYIRAGERGPLARVYVR"
BASE COUNT          676 a         1336 c         1352 g          650 t
ORIGIN      
        1 ccacgtcgcc ccccggctcc tcgatctcca agaagtcggt cacaccgcgc gggccgattg
       61 cccgcgtcgc cttcgtcgcc cctggaacgg acagccctgg aaaggacagc cacgggaaga
      121 gtgtggtgac gccgccgggc atcgcgccag gggccatctc catccccgga acgccgccga
      181 cgggcatcgc cgccgcgggg gcggccagga gcctgccgac aaactcctcc ggcttcattg
      241 ccgcgatctt cccctcgtac ttctggcgag gtggcaccgc gattccggcg ccggcaccca
      301 tcccgccggg cgccatgccc atcatcgggt cgccagccat gcccgcctcc atgccaggca
      361 tcgccgggcc gccgggcgcc attccaggga cacccatccc ctcggctcct ggcattccgg
      421 cctggccgcc ggtaggcacg ccggtgaagg tacgcgtcgg gtcgccgaac aactcggtca
      481 cctgattcgc gggccggccg agccggatcc ccgcgagctg gcgctcccgg gcgctcgcct
      541 gcgtcatcgc actcaggagt gcgcacgcgg tgagcgcggc cacccaagtg aaacgactgc
      601 cgcaccagaa ggggcggagg agttcgcttc ttgtaagatc tctagccatc gtgtcggcac
      661 ctttccttac gggcaaggag agtccccaat cccaatctgc gtattcgatt tccgcgaaga
      721 cgcatccttc tggtctattc tcgcgtaccc gaccgcgttc cttgtgtcac cagacagaga
      781 cgcgcatctg aggctcggcc cacggctcgg gctccaggtc aaccgcccca aagagtgaag
      841 ggtccaggaa atcgtctgcg cctccggccc acaccatctg catccgccgc ccgcgtggcc
      901 catccgcatc gtccaaagcc aggtcgaacc ccacgtagcg cgcacctggc atgcccagga
      961 gggagagggg gatgcgcaga tcgatccggt agccccctgg cattagggcg ctgtgggcct
     1021 ccagcgcttg ccaagcatcc gggagtggct ccagcggttt ccaagacgct ttttcggcat
     1081 gagacgttcc cggctgaatg aacagcccga caactccctg ctcccagtaa gggcgcgcct
     1141 gcgtgcctgc cggccggcaa tcgagaaaga gttcgacgca atcggaatcg aacgcgttgg
     1201 ggattcggcg gttgcgcacc acacggtcat cctccacggt caccgtcagg tgcaggaagt
     1261 ccccctcgcg tatgagagtc gccctccctg agagatcgtc cgggcctcgc cacccatccc
     1321 tgccaaggac aagcccccta ccatcgatca ccagcccatc ctgccccgcg cgggccaccg
     1381 gggtcgccgt cagccagcgc agatcataat cgaaggcgtc gcccgtgcgc gcccaggcga
     1441 aaagccgccg gcgtgccggc acgccctctt tcgcgaggga cacctggaag ctcaccggga
     1501 ccatcgtcct cgcgcccggg tcaacgacct gccgctgctc cacgccatca cactccagga
     1561 cgacggtgac aggatccggc cctggagcct ccaccaggac ctccacgggg gccgagtctg
     1621 cccgcgggaa gtagcgcgcg ccctcctgaa tgcgaacgcg cacgtggcgc tggcagggga
     1681 gcagcgtctc caccgcttcg tagagggatc gcgccatcac cgcatgcagg cgagcatcgg
     1741 gatgccggcc gaaagggaag ggcccgcgcg gtccggtgcc gcgcgctcgg atcgctccat
     1801 cgaaatcggc gaccggaatg ccagccctct tgccaatctc cttgaccgcg cgcaccatcg
     1861 gcgtgtcggc atcgggtgag acgatgggcg gggtggccag aagggccgcg gtgccaggct
     1921 gctcgcggat caagcgcacg atctcttcca gatcgcgctc aaactccgcg ggcgcgcgcc
     1981 ctcgcccgtg gtcattaccc ccgaactgaa tggtgaccag gtcggggtta tgcgagagaa
     2041 cgtcgctcgg gatgcggcga acgccgccgc gcgccgtatc gccgggaatc ccagcgggga
     2101 taagtcggat ccgtgctctt gggtagcggc gctggagcac actcgcgaag atggatgggt
     2161 aggcatcccg ctttgggtcc gaaagctcga agcccgcggc cagcgagtca ccaagggcga
     2221 ccacggtgat cgtctcgcca cgctccaggc gctcggccag acgcgcgtgc gcgggagcca
     2281 cgccgagtag cgccatcgca agcgcacatg cgctaatgaa gctgacccgc cgccggggaa
     2341 accgccgtat cgccaggcgc tggaccatcc acgttgcctc tactgccata ccttgccgga
     2401 cgcgccagac gatggcccgc gagatgcgcg tctctcctct ctcggccccc gggatacgag
     2461 ccccggctcc ctattggatt cgccacgatg tgcggcccct cctggtaccg ggaatagtgt
     2521 caggaagtat gggcaggaaa tgtcggggca cccggcagat cgcgtcctgg atgcaggagt
     2581 ccgcatcgcc tcgtggaaag ggggggtacc aaggcccttg ccgccggatc ccccggcgcc
     2641 tacggcaagg attctcgccg cgggttgtgg attaccgggc atctgagacg gcgcgtgccc
     2701 ggtgtggcac gcgtgctccc tgctggaggt gccgttggac aggcgcggca acagatcgct
     2761 tctttccgga gggatggtgc gttcgtatgg caagtcaaga agtcctatgg cccatctacc
     2821 agattcagaa gcttgatgca cagatcgcct tgttgcagcg cgcgcggggg gcgttggatg
     2881 atggcgcggc cctggcagcg gaggtaaccc aacaccgtgg cgatattctc tctcttcagg
     2941 agagcctgcg ccaggtcgac cgggcgatac gagagcgcga actagagctg gcgtccctgg
     3001 aagaaaagct tcagaaggtc gaggcgatcc tttacagtgg caaggtgacg aacccgaagg
     3061 agcttcgggg gtatgagggc gagcgcgagt cgttgcgccg ccggcgcgag cgtctcgaag
     3121 aagagacgct gattcggatg gaggagaagg actcgctgaa cgaacgcctg gctgccgccc
     3181 agaaggtgga gaaggaagcg tatgctcggt ggcaggcgca cgtggcgaag tttcgcgccg
     3241 atgtagcggc gcttgatgcg aagtacgcga agctcgtcgc cgagcgcgat gaggtgcgcg
     3301 cgcgcgccga tagcgagttg ttgcgtcgct atgaccagct ggcacgccag cggaagcagc
     3361 tggtgggact ggtggagcag aacgcgtgcg gggcgtgtgg tatgcttctc ccggcgcgtc
     3421 tggtgaagga gtatcaggtg aaccccgatg ccgagctcac ctgccccacg tgttcggcac
     3481 acctcctctg gcggctcccc ggcgcggatt cggcgcgacc tgcctgattg gcacgggtct
     3541 cgcacccatc gtttccagag acgggttgca gggtaagcca tttctgggag attggcgtgg
     3601 tgatgcggcg aagctgccgc gcatgggatg gcgtgtgatg cgtgcgagtg gtctgtggtg
     3661 ggtggttgtg gtggctctcc tgggtggggg gtgcgattcc ctctcgcgta tgaaactggc
     3721 gggcggggcc gacattgccg cggactctca ggatgcgcgg gtgacgcacc cgcagcgaag
     3781 tctggcgaca ctgctcgagc ggcagggtct cccgcagacg ttgctatgca gcggcacgac
     3841 ctggagagcg gttggtctgg gcggctttcg caacgagcag cgcctcgcgc cggtgaagcc
     3901 cggcattgct ggctatacgc tgcttcacga gcgcggcgcg cggcgtgatg agcccccgcg
     3961 cctctacatt cgggcggggg agcggggccc cctggcccgc gtttacgtgc gcta
//
LOCUS       DAOSCV010000353         4010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14162_length_4010_cov_3.919343, whole genome shotgun sequence.
ACCESSION   DAOSCV010000353 DAOSCV010000000
VERSION     DAOSCV010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14162_length_4010_cov_3.919343"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..3098
                     /locus_tag="PLY56_09560"
     CDS             33..3098
                     /locus_tag="PLY56_09560"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ21768.1"
                     /translation="MRTATFFAFVLFMLPVAALAQESGPVTIGGVRYERVDPGEPFQE
                     RPAPKPDWQPPQPTSSERAAGLLAYITSDPGQYRPDGVPRPEEHARELTAFFTPGEDE
                     PVWVGTRALESLKGLAVRVDLADTPVTCDVRHMHFWPQRTGWRSRQWYITPELLLPCR
                     DGRKTVPVTRGVLEERAFDLAAGETAAFWLTLSARPDARPGLYTARVSIQAAGKPALV
                     LPLRIEVLPFQLQRPKDRAWLLYADAGRWRAMTDAQVMAELRDFARHGITGLVEGSLG
                     TADLSGLKEGRVVFDASSYKKLAAQCLEAGIPGPHVCSMGGWPQRVRDALGLKVDLEK
                     GTWPEEIRAGVTAVAKAAVEATKDAPVPWLFYGRDEPSGEDTYAIQDYQCWRAGGATT
                     YATFYQIGFLEKASEYLTAPCFVAGLVANEERARKAREECARTGAEFWWYGTGCYVNP
                     FPQEGYMFHNRYGAGLLFWKTGAKAQVSWTFCRPHEDVFNDFDGSRANRVEPKEQCTV
                     YPHLLKPDDWSTYQGAIPTIAWEALREGVDDYRYLYTLSQTIAEARAHRDERVRAAAR
                     EAQEMLDALVNAVPWANPLGGVAFETSRMQQVRRAAADQILRLRAIMTGKPVAQRRQD
                     ASQVTLVVSATEGASTRALPAIPVLPTTMPPVIDGDLSDPCWAESAVATNFYRAHDGG
                     KPEVDTEARILYDARALYVSFRCPEPAMARLRAIRHGHDTPEIWLDDGVEFFVAGGPR
                     APYAHLIVNTNGAVYDERNQDPSWNSSAEVSVRKEASGYSVEIALPWSELEKAGVRRS
                     PVMAVNFCRSRFAGPEADRLSAWSCTYDGFHVPERFGWALVQEGSLALQSLALPRFWG
                     KQSVEVVLQNRGETPAEARLALENQEQSLRLAPGASGTARFPVNLRQPGARELRFAWG
                     QSGRPASEVTLPVTVPEPAILPRAGGLASDGETLELPVAVHLAASEQKSYRIRVEAGA
                     RRVEIPAKPGAQKRVRLKAGGPVRLRVSLVDRNGAPVGESAEQTLVVLP"
     gene            complement(3117..>4010)
                     /locus_tag="PLY56_09565"
     CDS             complement(3117..>4010)
                     /locus_tag="PLY56_09565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ21769.1"
                     /translation="KPRRRGFISHAHTDHYAAHETIFCTPETADMLALVKPFEEELPP
                     IRPLYLGRTIEHGDLQVTLLPSGHILGAAMMHVTRGGESLLYSGDVRPSGSATTPPAQ
                     VVPADHLIVEDTFGALDEEFVGAEEGAARVVEFCRKCRARNRTPILVTTSNCGKAQEM
                     VVALGQAGLAVALQSKIWHFTRLYERHGIPVSGYTRLRKDEWPDADAVIVTRAYLEHD
                     DLTDRVPRPTYALVSGWAASDEADDFDAAIPWSDHASREELLAFIEAVNPRLVWTFAG
                     EGHLLPELRAAGRDAVHLEAC"
BASE COUNT          620 a         1356 c         1406 g          628 t
ORIGIN      
        1 atgcgggcta tgccggcgat caggagagac cgatgagaac tgcaactttc tttgccttcg
       61 ttctattcat gctgccggtg gcagcgctcg cgcaggaaag cgggccggtc accatcggcg
      121 gcgtccgcta cgagcgcgtg gacccaggcg agccgttcca ggagcgtccg gcaccaaagc
      181 cagactggca gccgccacag cccacctcat ccgagcgcgc cgccggactc ctcgcctata
      241 tcacgtcgga cccgggtcag taccggccag acggagtgcc acgccccgag gagcacgcgc
      301 gcgagctcac cgctttcttc acgcccggcg aggatgagcc ggtgtgggtg ggcacgcgcg
      361 cgctggaaag cctgaagggg ctcgcggtcc gtgtcgatct cgccgacacc ccagtgacgt
      421 gcgacgtgcg gcacatgcac ttctggccgc agcgcaccgg atggcgctcg cgccagtggt
      481 acatcacccc ggagctgctc cttccgtgca gggacgggcg gaagacggtg ccggtgacgc
      541 gcggcgttct ggaggagcgg gcgttcgacc tcgccgcggg agagacggcg gcgttttggt
      601 tgactctgag cgcccgcccg gatgcccggc ctggccttta caccgcgcgc gtctcgatcc
      661 aggcggccgg caagcccgcc ctggtgctcc cgctgcgcat cgaggtgctc cccttccagc
      721 tgcagcggcc caaggaccgc gcgtggctgc tctatgccga cgccgggcgc tggcgcgcga
      781 tgaccgatgc ccaggtgatg gccgaactcc gcgatttcgc tcgccacggg atcaccggtt
      841 tggtggaggg ctccctcggt acggcggatc tctccggatt gaaggagggc cgggtggtct
      901 tcgacgcatc ctcctacaag aagctcgcgg cccagtgcct tgaggccggc attcccggac
      961 cgcacgtctg ctccatgggc ggatggccgc agcgcgtccg tgatgcgctc ggtctgaaag
     1021 tcgacctgga gaagggaacc tggccggagg agatccgcgc cggcgtcacc gccgtggcaa
     1081 aagccgccgt cgaggcgacg aaggacgcac ccgtgccctg gctcttctac gggagagacg
     1141 agccgtccgg cgaggatacg tacgccatcc aggattacca gtgctggcgc gcgggtggcg
     1201 ccacgaccta cgccaccttc taccagatcg gtttcctgga gaaggcctcc gagtacctca
     1261 cggcgccctg cttcgtcgct gggctggtgg ccaacgagga gagggctcgg aaggcgcgcg
     1321 aggagtgcgc gcgcaccggc gctgagttct ggtggtacgg caccggatgc tacgtcaatc
     1381 ccttccccca ggaaggctac atgttccaca accgctatgg cgccggactg ctcttctgga
     1441 agacgggcgc gaaggcacag gtctcgtgga ccttctgccg gccgcacgag gatgttttca
     1501 acgacttcga tggctcgcgc gcgaaccgcg ttgagcccaa ggagcagtgt accgtctacc
     1561 cgcacctcct caagccggac gactggagca cctatcaggg cgcgatcccg accatcgcct
     1621 gggaggcgct ccgcgaaggc gtggatgact accgctacct ctacacgctc tcgcagacga
     1681 ttgccgaggc acgcgcgcac cgcgacgagc gcgtgcgcgc cgctgcgcgc gaggcccagg
     1741 agatgctcga tgcgctcgtg aacgccgtgc cctgggccaa cccactgggg ggcgtggcgt
     1801 ttgagacgag ccggatgcag caggtgcgcc gggccgccgc ggaccagatc ctccggctgc
     1861 gggccattat gactggcaag cccgtggcgc agcggcgcca ggacgcttct caggtcacgc
     1921 tcgtggtgag cgcgacggaa ggagcatcca cgcgagccct accggcaatc ccggtgctcc
     1981 cgaccacgat gccgccggtg atcgatggcg acctttccga cccctgctgg gccgagagcg
     2041 ccgtcgcgac caacttctac cgggcacatg atggcggcaa gccggaggtc gacacagagg
     2101 cccggatcct ctatgacgcg cgcgccctct acgtttcgtt tcgctgcccg gagccggcga
     2161 tggcccggct ccgggccatc cggcatggcc atgatactcc agaaatatgg ctggatgacg
     2221 gcgtcgagtt cttcgtggcc ggggggccgc gcgcgcccta tgcccatctc atcgtgaaca
     2281 ccaacggcgc ggtgtatgac gagcgcaacc aggacccgtc gtggaactcc tccgccgagg
     2341 tgagcgtgcg caaggaggcc agtggctact cagtcgagat cgccctgccc tggtcggagc
     2401 tggagaaggc gggggttcgg cgctcgcccg tgatggcggt caacttctgc cgcagccggt
     2461 tcgctggccc ggaagcggac cggctttcag cgtggtcgtg tacctatgac gggttccacg
     2521 tgcccgagcg gttcggatgg gcgctcgtgc aggaggggag cctggcgcta caatcgctgg
     2581 cgctcccgcg gttctggggc aagcaatcgg tggaggtggt gttgcagaac cggggcgaga
     2641 cacccgccga ggctcgcctc gccctggaga atcaggagca aagcctccgg ctggctccgg
     2701 gtgcgtccgg cacggcgcgc ttccccgtca acctgcgcca gcccggcgcg cgcgagctac
     2761 gcttcgcctg gggccagtct gggcgccccg cgagcgaggt cacactcccg gtcaccgttc
     2821 cggagccggc gatcctcccg cgcgccggtg gcctggcctc ggatggcgag acgctggagc
     2881 taccggtcgc cgtacacctc gctgccagcg agcagaagag ctatcggatc cgcgtcgagg
     2941 ccggcgcacg ccgcgtcgag attccggcga agcctggcgc gcagaagcgc gtgcgcctga
     3001 aggccggcgg cccggttcgc ctgcgcgtct ctctggtgga tcgcaacggc gcgcccgttg
     3061 gcgagagcgc cgagcagacg ctcgtcgtgc tcccgtgacc gaagtcccgc ctggatttag
     3121 cacgcctcca ggtgaaccgc gtcgcgtccg gcggcacgga gctcagggag caggtggcct
     3181 tcgcccgcga aggtccatac caggcggggg ttgacggcct cgatgaacgc gagcagctcc
     3241 tcgcgggagg cgtggtcgct ccacgggatg gcggcgtcga agtcatcggc ctcgtcggac
     3301 gcggcccacc cggagacgag ggcgtaggtc ggccggggca cgcggtccgt caggtcatca
     3361 tgctccaggt aggcgcgggt gacgatcacg gcgtcggcat ctggccactc atccttgcgg
     3421 aggcgcgtgt agccggaaac cgggatcccg tggcgctcgt agagccgcgt gaagtgccag
     3481 atctttgact gcagcgccac ggcgagcccc gcctgcccaa gggcaacgac catctcctgc
     3541 gctttcccgc agttgctggt ggtcaccagg atgggcgttc tgttccgtgc ccggcacttg
     3601 cggcagaact cgaccacacg ggcggcgccc tcttccgcgc cgacgaactc ctcatctaga
     3661 gcgccgaagg tgtcctccac gatcagatgg tcggccggca ccacctgggc cggtggcgtc
     3721 gtcgcggatc cggacgggcg cacatcacca gagtagagca gcgattcgcc cccgcgcgta
     3781 acatgcatca tggcggcgcc caggatgtgc ccgctgggca ggagggtgac ctggaggtcg
     3841 ccatgctcga tggtccggcc caggtacagc gggcggatgg gagggagctc ctcctcgaat
     3901 ggcttcacga gcgccagcat gtcggccgtc tccggcgtgc agaagatcgt ttcgtgcgcg
     3961 gcgtagtgat ccgtgtgcgc gtggctgatg aagccgcgcc ggcggggctt
//
LOCUS       DAOSCV010000354         4010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14164_length_4010_cov_3.339823, whole genome shotgun sequence.
ACCESSION   DAOSCV010000354 DAOSCV010000000
VERSION     DAOSCV010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14164_length_4010_cov_3.339823"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..1749)
                     /gene="glmM"
                     /locus_tag="PLY56_09570"
     CDS             complement(391..1749)
                     /gene="glmM"
                     /locus_tag="PLY56_09570"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ21770.1"
                     /translation="MTRFFGTDGLRGRANVDLTPELAVRVGLAAGFVLGEGEPGILIL
                     GRDTRESGTMLEAAVASGLASAGWNVRLAGVMTSPGVAYLTNLLGADAGCVISASHNP
                     APDNGIKFFTAEGAKLVPAVEEQIEALLDPEVLAHQERPRPGWIRPLKDAGRRYTEAL
                     AEEVRADLTGLRIVVDCANGAASRLAPGLFARLGAEVIPLCHQPDGRNINAGCGATHP
                     ERLASTVLREGADAGVAFDGDADRAIFVDHEGQVRNGDHVLYIIGTQRRAQGKLPGDL
                     VVGTVMSNLGAERALAAQGIRLERTRVGDREVFARMCETGAVLGGEQSGHIIFRDRLN
                     TGDGILTALEVFTAVRKAGKPLAELAAPVELYPQLLINIPLRPGLEWQDSSPLGQALK
                     EVKALVEPEGRIVVRPSGTEPLLRVMIETPHQELLDQASQRIQTADDFPGLTCTLLSP
                     AG"
     gene            complement(1746..2687)
                     /locus_tag="PLY56_09575"
     CDS             complement(1746..2687)
                     /locus_tag="PLY56_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOJ21771.1"
                     /translation="MIRHNLRYKVLALASALALYFMYGRTDEKPLTSAVVRVQVRAVN
                     VPDGLEITTQPVISVSIQGPKHAIDELLNRPDGGASELDATVDLAGKGVGPHMLPVKV
                     NLRKPLMLVPGQLYATVTLEAIVERTLPVEVVPQGSVAPGYRWESTSANPASITIVGA
                     RSAVEQVARVEARVPVRGATGDLERDVPLRALDSRDEVVEHVDVRPSLVSVHVRISRL
                     LRYKVAPIVLQTTGRPANGYRISTVVVEPLVVTVSGSSQALESVQAVSTEPVDLTGAT
                     APIIRRVRLAPPEGIETVSASAARVKVFLVNEAVGTP"
     gene            complement(2684..3718)
                     /gene="cdaA"
                     /locus_tag="PLY56_09580"
     CDS             complement(2684..3718)
                     /gene="cdaA"
                     /locus_tag="PLY56_09580"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ21772.1"
                     /translation="MLKGVTHFVTNLPWQAAFDVLISTVIIYYVIMLIKGTRAVQLAW
                     GLVLLFLFARIADWVEFRTLSLLLGTIATPGATVAIVILFYPELRHALEEVGRGRFWA
                     MRGLMLRREDVSAVVDELIETAQRLSQRRFGALMVIERDDGLDDIATTGTALGATVTP
                     RLLETIFYHGTPLHDGAVIIRGNEVIAAECVLPLSDNSRISSSLHTRHRAALGLAETT
                     DAAVLVVSEETGNISLAYDGRLLTALRPEVARDKLLSLLQRDESLEGRRGNDPGSAGG
                     KGAGSATPKEEAIPEPPEVAGVAAVSALEEPRTLRGMLYSGLRRARGLFGIGNRVSGL
                     RSLFRGEPSD"
BASE COUNT          697 a         1355 c         1313 g          645 t
ORIGIN      
        1 ttgccggaaa gcagggtgga gcttcgagct ccgcgcgtgt ggacggcctt ctccttatgt
       61 cggaacccca gaggggcaga cgcctgatag cgtcgcgcga gcttgccctt gttatacgca
      121 gatagacaca gatggatgag gattggggat atcaccggag ccactcctca ccgtcccggt
      181 gcatcccctt atccttggta ttgccgccgc ccggcggggg aaagattccc gaccccaaat
      241 ggcgcgcaca acccgggatg ggtacacccc gcccatccgt gtccttcatc cgtggtatcg
      301 atgccggtgg ggaaccggat ggccgggcat cgcgcgggcg taaaccggcc ccgcgcgatg
      361 cggaagtggg tgtgacgcta cctctccccg tcagccggct ggcgagagga gcgtgcaggt
      421 gagtccgggg aagtcgtccg cagtttggat ccgctgagag gcctgatcca gaagctcctg
      481 atggggcgtc tcgatcatga cgcgcaggag cggctcggtg ccagaggggc ggaccacgat
      541 gcggccttca ggctcgacga gggccttgac ctctttcagc gcctgcccaa ggggcgagct
      601 gtcctgccac tccagtccgg ggcgcagggg gatgttgatc aggagctgcg ggtacagctc
      661 caccggggcg gccagctccg ccagcggctt gccggccttc cggaccgcgg tgaacacctc
      721 cagcgcggtg aggatgccat cgcccgtgtt caggcggtcg cggaagatga tatgccccga
      781 ttgctcgcca ccgagtacgg cgcccgtctc gcacatgcgc gcgaagacct cgcggtcgcc
      841 cacgcgggtg cgctcgaggc gaattccctg tgcagcgagg gcacgttcgg caccaaggtt
      901 ggacatcacc gtgcccacga cgagatcgcc gggaagcttg ccctgggcgc ggcgctgcgt
      961 gccgatgatg tagagcacgt ggtcgccgtt gcgcacctgg ccctcatggt cgacaaagat
     1021 cgcccggtcg gcatcgccat cgaacgccac accggcatcg gcgccctcgc gcaagacggt
     1081 gctggcgagc cgctccggat gggttgcccc gcacccggcg ttgatattgc ggccatcggg
     1141 ctggtggcac agcgggatca cctcggcccc caggcgagcg aacagcccgg gcgccaggcg
     1201 cgaggcggcc ccgttggcac agtcgacgac gatgcggagc cctgtcaggt cggcccggac
     1261 ctcttctgcg agggcctccg tatagcgccg gccggcatct ttcaacgggc ggatccaccc
     1321 cgggcgcggg cgctcctggt gagccagcac ttccggatcg aggagagcct ctatctgctc
     1381 ctcaacggcg ggcaccagct tggcgccttc cgcggtgaaa aacttgattc cgttgtcggg
     1441 ggcgggattg tgcgacgcgg agatgacgca gcccgcgtcc gctcccagga ggttcgtgag
     1501 gtaggccacg ccgggggagg tcatcacgcc tgccaggcgc acgttccaac cggcgctagc
     1561 caggccagag gcgacggcgg cctccagcat cgtcccggac tcgcgcgtgt cgcgccccag
     1621 gatgaggatg ccgggctcgc cctcgccgag aacgaagccc gcagcgagcc cgacgcgaac
     1681 tgccagctcg ggtgtcagat ccacattggc tcggccacgc aggccatcgg tgccgaaaaa
     1741 tcgcgtcatg gagtacctac cgcttcgttc accaggaaga ccttcacccg cgcggcagac
     1801 gcggaaacgg tctcaatgcc ctctggcggc gccaggcgaa cgcgccggat gatgggagcg
     1861 gttgctcccg tgagatccac aggttcggtg gacaccgctt gcacactctc cagggcctgg
     1921 ctgctccccg ataccgtgac aaccaacggc tccacgacca ccgtcgatat ccggtaccca
     1981 ttcgccgggc gcccggtcgt ctggagcaca atcggtgcca ctttgtagcg cagcagtcgg
     2041 gagatgcgca cgtggaccga gacgagggat gggcggacat cgacatgctc caccacctca
     2101 tctcggctgt ccagagcgcg cagcggcaca tcgcgttcaa gatcaccggt ggcaccgcgc
     2161 acgggcacgc gcgcctccac tcgggccacc tgctccacgg cacttctggc ccccacgatg
     2221 gtgatcgagg ccggattggc actggtcgat tcccagcgat aaccgggagc gacactcccc
     2281 tggggtacca cctcgaccgg cagcgtccgc tcgacaatcg cctccagggt gacggttgca
     2341 tacagctgcc cgggcaccag catcagcggc ttgcggaggt tcaccttcac cgggagcata
     2401 tgcggtccga cgcccttgcc ggcgagatcc accgtcgcat cgagttcgga ggcgccgcca
     2461 tccgggcggt tgagaagctc atcgatggcg tgtttggggc cctggatgct cacggagatg
     2521 accggctgcg tggtgatctc cagcccgtcg ggcacattca cggcgcgcac ctgcacgcgt
     2581 acgacggcgg acgtaagcgg cttctcatcc gtccgcccgt acatgaagta aagcgcgagc
     2641 gcgcttgcca gggccagaac tttatacctg agattatgcc ggatcaatcg ctcggctctc
     2701 cacgaaacag actgcgtagg ccagacaccc ggtttccaat cccaaacagt ccgcgcgcac
     2761 ggcgcaggcc ggagtagagc atgccgcgca acgtgcgggg ttcctctagc gcgctcacgg
     2821 cagccacccc cgccacttct ggcggctccg ggatcgcttc ttcctttggc gtggcagagc
     2881 ccgcaccttt gccgccggcc gacccggggt cgttgccacg gcgcccctcc aggctttcat
     2941 cacgctggag caggctcagg agcttgtcgc gcgccacctc cgggcgcagc gcggtgagga
     3001 ggcgtccatc gtaggcaagg gagatgttgc ccgtttcctc cgagacgacg agcacggccg
     3061 cgtctgtcgt ctccgcgagg cccagcgccg cgcgatgccg cgtgtgcagc gaggagctga
     3121 tccgcgagtt gtccgagagc ggcaacacgc actctgcggc gatcacctcg ttcccccgga
     3181 tgatcacggc gccgtcgtgc agcggcgtgc catggtagaa aatcgtctcc aacagccgcg
     3241 gcgtcacggt agcccccaga gcggtcccgg tcgtcgcgat gtcatcgagg ccatcatccc
     3301 gctcgatcac catcagtgcc ccgaaccggc gttgtgagag ccgttgcgcg gtctcgatca
     3361 gctcgtcgac tacggcactc acatcttcgc ggcggagcat caacccgcgc attgcccaga
     3421 accgaccgcg ccccacctct tcgagcgcgt gacgaagctc cgggtagaac aggatcacga
     3481 tggcgaccgt cgcccccggg gtggcgatgg taccgaggag aaggctgagc gtgcggaact
     3541 ccacccagtc cgcaatccgc gcgaaaagga agaggaggac caggccccag gcgagctgaa
     3601 cggcacgtgt tcccttgatc aacatgatca cgtagtaaat gatcacggtc gagatgagca
     3661 cgtcgaaagc cgcctgccag ggcaggttcg tgacgaagtg ggtcacaccc ttgagcatct
     3721 gcatccctgg agcgcgttct ccgcggcgcc tggccgctgc gcccgagaac gcgcgctgaa
     3781 cctgttgcct acccgttcgg gatcgcgccg gcacgtcgcc ggatacactg aaaggggact
     3841 ttatgccccg gaggcataac gggtggctac gcccgattgc ggggaaggaa ccggcgttca
     3901 ccccgccagg attccgcgat ggcccgaacc acgtcgcaac catgttacca tatcgcggag
     3961 gatagggtca acgccgctcc tgcagttttg ttagaaaagt cagcggggca
//
LOCUS       DAOSCV010000355         4003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14196_length_4003_cov_3.681611, whole genome shotgun sequence.
ACCESSION   DAOSCV010000355 DAOSCV010000000
VERSION     DAOSCV010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14196_length_4003_cov_3.681611"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3977)
                     /locus_tag="PLY56_09585"
     CDS             complement(<1..3977)
                     /locus_tag="PLY56_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOJ21773.1"
                     /translation="MDDVNVESGVELPADAVIPEPVVPVYDRLKELHLRTDLVRGGQP
                     AITIAAPSRYRAEAGAIQAAIREVTGVTVPVVSDAAPEAAVPLKGHLILLGNRSTNRA
                     ISALYDRAYTFLDLKYPGLGGYVVRSLHSPFGDGRNVLFAGGSDDEGVRRAAEALAAK
                     LRAAGGKQGELSVGWLMEIRLGRGLSLPKDVRQMELWEASRMYGSSGYFGWNILSKLM
                     AAYYMTGEEWCLREFLRYAFPDARAIKELEEWDGERVENKSAPLSGPYHYSAHMMILL
                     WDLIEESPLLTDADRLRITNAFAKQLPHRVVEGVYAATSLPAYVGDRHRDWSAVSLYC
                     LGRYFQKDYPSPVWERCITATHQYFSALRDHHWMAGMNDHLFWYTSFYDPMLNYMILS
                     GDRTAVENGNLVKALRTQEILSTGLDADWGLKASSLSFLNKTAYLTGDGRWLYYRERT
                     GLDTDCFRLGQSFWPGDELKPQAPEDLVGRWTIQAMPKPMWLQRASGIPLEQSFRWGS
                     YRSTLGPDGDLVMIKGYNGAGRLPYHTCVLMELRLNGMTLLQGYRNQILSSADGMVEP
                     QVPLDGALLHHGVLGQTASVVLEVPRLPFNRWRRSLAQRVGHYALIVDDLTFRTDSDN
                     MKLEVSWEMPGAKWDPKAGVVRVPRKAGVGLPPGWMHLPALRSEKVACGPGTPQELLS
                     RLHDLGILLLKAPGPGTWVETSFTLAKPFRGEVYVDLLNYRDRGQICFLLDGRPVGSE
                     VDHYDLGAVPAQVSLGTHALAAGEHVLRVEVTGKRAEATRHYVGLVGVSLRPEGAPAQ
                     STSAVYELRSSEPLPFTRRDDALVMEWRGPVRDGGRRFFFHLLGCNPDGSEEGLACAR
                     IAENAAALALPEPALAVAGSYGGCEGELVILAYTHLYARAARRVGLKAPLLAADQPVD
                     IDWDFATGELHVEAGTGATVRLALAPGRVLLDGKPVSGQADADGLLAVRLPAGRHLVQ
                     GARPRTDLSAGLATLLAEARRQRAVLVAASAAPGPKAPEWPPAARARVSGKPGSSAVV
                     PAPDGPLLCVAVEKAIHLLGPDGKEAARFEADAEVRVVHWWPEAKLLLAGCVDEKVIA
                     FDLRGQRRWVFTSEMDRAVYEAGKQYWFKSAHPGIYGLSTGRFVDGQSQCFVGSACTL
                     EILDMEGKLVKRLPTFWGPGRQFLLLDAPDGSRNLLIARWRNDSAAMAIVNSKTLTRT
                     GTGFYGVPDGHTFIGGWDCQNREDNYVIDLDGDGKVEVVSAINGTWNRVTVWNAAGEP
                     LYNAQFGPGNTAPRANLRGMDVADLNGDGRREIIVGTSGGLGRARDHTFRHWWSRATT
                     SPPVVL"
BASE COUNT          665 a         1414 c         1304 g          620 t
ORIGIN      
        1 agaaccaccg gcgggctcgt ggtcgccctg gaccaccagt gccggaaggt gtggtcgcgc
       61 gctctgccca gcccgccgga ggtgcccacg atgatctcgc gccggccatc accgttcagg
      121 tcggccacgt ccatgccgcg cagattggcg cgcggcgcgg tgttgcccgg cccgaactgg
      181 gcgttataca gcggctcgcc cgccgcgttc cacaccgtga cgcgattcca cgtgccgttg
      241 atcgcgctga ccacttccac cttgccgtcg ccatccaggt cgatgacgta gttgtcttcg
      301 cgattctggc agtcccaacc gccgatgaag gtatgcccgt ccggcacccc gtagaatccg
      361 gtgccggtgc gcgtcaacgt cttgctgttg acaatcgcca tcgccgcact gtcgttgcgc
      421 cagcgcgcga tgaggaggtt gcggctgcca tccggcgcgt ccaggagcag gaactgccgg
      481 cccggccccc agaaggttgg caggcgcttg accaacttcc cctccatatc gaggatctcc
      541 agggtgcatg cgctgccaac gaagcactgg ctctggccgt ccacaaagcg cccggtggaa
      601 agcccgtata tgcccggatg cgcgctcttg aaccagtact gcttccccgc ctcatacacc
      661 gcgcggtcca tctcggaggt gaagacccac cggcgctgcc cgcgcaggtc gaaggcgatc
      721 actttctcat ccacgcaccc ggcgaggagc agcttcgcct ccggccacca gtggacgacg
      781 cggacctcgg catccgcctc gaaccgggcg gcctctttgc catccggacc gagcaggtga
      841 atcgccttct ccacagccac gcagaggagc gggccatccg gtgcgggcac cacggccgaa
      901 ctgcctggct tgccggagac gcgcgcccgc gccgccggcg gccactccgg agccttcggg
      961 cccggcgccg ccgacgccgc gacgagcacc gcgcgctgcc gccgggcctc ggcgagaagc
     1021 gtagccagcc cggcgctcaa atccgttcgg ggccgcgccc cctgcacgag atgccgtccg
     1081 gcgggaagcc ggacggcaag gaggccatcc gcgtccgcct ggcccgagac gggcttccca
     1141 tcgaggagca cgcgcccggg cgccagggcc aggcgcacgg tcgccccggt gccggcctca
     1201 acgtggagct cgcccgtggc gaaatcccaa tcgatatcca ccggctggtc ggcggcgagg
     1261 agcggtgcct tcagcccgac gcgccgcgcc gcgcgcgcgt aaagatgggt ataggccagg
     1321 atcaccagct ccccctcaca gccgccgtag ctcccggcaa cagcgagcgc aggctcggga
     1381 agcgccaggg ccgcggcgtt ctccgcgatc cgcgcgcacg ccagcccctc ttcagagcca
     1441 tccgggttgc atcccaacag gtggaagaag aagcgccggc ctccatcgcg caccgggccc
     1501 cgccactcca tcaccagtgc gtcgtcgcgg cgggtgaagg gcagcggctc ggaggaacgc
     1561 agttcataca ccgcggaggt cgattgcgcc ggtgcgccct ccgggcggag agagacgcca
     1621 acgaggccca cgtaatgcct tgtcgcctct gcgcgctttc cggtcacctc cacgcgcaac
     1681 acatgctcgc cagccgcgag cgcgtgcgtg cccagcgaaa cctgcgcggg caccgcaccc
     1741 aggtcgtagt ggtccacctc gctgcccacc gggcgcccgt ccagcaggaa gcagatctgc
     1801 ccccggtcac ggtagttgag aaggtccaca tacacctcgc ctcggaacgg cttcgccagc
     1861 gtgaaggagg tctcgaccca cgtgccgggc cccggagcct tcagcaggag gattcccagg
     1921 tcgtgcaggc gcgagagcag ctcctgcggc gttcctggcc cgcacgccac tttctcgctc
     1981 cggagcgcgg ggaggtgcat ccacccgggg ggcagtccga cgcccgcctt tctgggcacg
     2041 cgtaccactc ccgcctttgg atcccacttc gccccgggca tctcccagct cacctccagc
     2101 ttcatgttgt cgctgtcggt gcggaaggtg aggtcatcca cgatgagcgc gtagtgcccg
     2161 acgcgctgcg caagcgagcg tcgccaccgg ttgaagggga gccgcggcac ctccagcacg
     2221 acgctggccg tctggccgag gactccgtga tgaagcagcg cgccatcgag ggggacctgg
     2281 ggctcgacca tgccatcggc gctcgaaagg atctggtttc ggtagccctg aaggagcgtc
     2341 atgccgttca ggcgcagctc catcaggacg caggtgtgat atgggagacg gcccgcgccg
     2401 ttatacccct tgatcatcac caggtcgcca tcggggccca gggtgcttcg gtagcttccc
     2461 cagcggaacg attgctccag cggaatgcca ctcgctcgct gaagccacat cggcttcggc
     2521 atcgcctgga tcgtccaccg acccacgaga tcctccggcg cctggggctt cagctcgtcg
     2581 cccggccaga acgattgccc caggcggaag caatcggtgt cgagcccggt gcgctcgcga
     2641 tagtagagcc agcgtccgtc gcccgtcagg taggccgtct tgttgaggaa ggagagcgac
     2701 gacgccttca ggccccaatc cgcgtccaac ccggtggaaa ggatctcctg ggtgcgcagc
     2761 gccttgacca ggttcccgtt ctccacggcg gtccggtcgc cgctgaggat catgtagttg
     2821 agcatcggat cgtagaacga cgtgtaccag aagaggtggt cgttcatccc cgccatccag
     2881 tggtgatcgc gcagcgcgct aaagtactgg tgggtggcag tgatgcaccg ctcccagacg
     2941 gggctggggt agtccttctg aaagtaacgg cccaggcagt agagcgagac ggcggaccag
     3001 tctcgatgcc ggtcgcccac gtaggccggg agcgaggtcg cggcatagac gccctcgacg
     3061 acgcgatggg gaagctgctt cgcgaaggcg ttggtgatcc gcagccggtc ggcatccgtg
     3121 aggagtgggc tctcctcgat gagatcccac aggaggatca tcatgtgcgc ggagtagtgg
     3181 taggggccgc tgagaggcgc gctcttgttc tcaacgcgct cgccatccca ctcctcgagc
     3241 tctttgatag cgcgcgcgtc cgggaaggcg tagcgcagga actcgcgaag gcaccactcc
     3301 tcgccggtca tgtagtaggc ggccatcagc ttgctgagga tgttccagcc gaagtagccg
     3361 gaagagccat acatccgcga ggcttcccag agctccatct ggcgcacgtc tttcgggagc
     3421 gacagcccgc gcccaagccg gatctccatc agccagccca ccgagagctc gccctgcttc
     3481 cccccggcag cgcgcagctt cgccgcgagc gcctctgcag cacggcgcac gccctcgtca
     3541 tcgctcccgc cggcgaagag gacgttccgg ccatcgccaa acgggctgtg cagcgagcgg
     3601 accacgtagc cgcccagacc cggatacttc aggtcgagaa aggtgtaggc gcggtcgtaa
     3661 agcgcggaga tcgcccggtt ggtggagcga ttcccgagga ggatgaggtg gcccttgagc
     3721 ggcaccgccg cctcgggcgc cgcatccgag acgaccggca ccgtgactcc cgtcacctcg
     3781 cggatcgcgg cctggatcgc gccggcctcg gcccggtagc gcgatggcgc ggcaattgtg
     3841 atcgcgggct gtcccccgcg caccagatcg gtgcgcaggt ggagctcttt caggcggtca
     3901 tagaccggca caaccggctc gggaatgacc gcgtcagcag gcaattcaac gccagattcc
     3961 acattcacat catccactat caccttcgga gtggtggcct tgt
//
LOCUS       DAOSCV010000356         4001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14203_length_4001_cov_3.532438, whole genome shotgun sequence.
ACCESSION   DAOSCV010000356 DAOSCV010000000
VERSION     DAOSCV010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14203_length_4001_cov_3.532438"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLY56_09590"
     CDS             complement(<1..727)
                     /locus_tag="PLY56_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21774.1"
                     /translation="MRERVEEVAPLAGAGRAITVRSVLISLIVTFLTGFWVRESELEA
                     VGVFISESVPTIPAVGSILLLLLINSFLRGSRHAFSRGELIATFFMVTVATTPYNCGM
                     VRILLAFITAPFYFAGAGNRLGETHPLIPAWATPRDPEVVRGFYEGLRHAPIPWGAWV
                     VPVLAWVGFFLALWLAMACLVALMHRPWVEKEKLAFPLVQLPLEMTEPGGRGVPAFFS
                     NPVMWAGFAVAFVADGAHILHALI"
     gene            913..>1109
                     /locus_tag="PLY56_09595"
     CDS             913..>1109
                     /locus_tag="PLY56_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21775.1"
                     /translation="MTTRPDYMRARDEAGFRWHVSASQGALREVTGIPIREFNLNPEA
                     CIEAYRRGRPLLRELFGPEVRL"
     assembly_gap    1110..1119
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1163..2152
                     /locus_tag="PLY56_09600"
     CDS             1163..2152
                     /locus_tag="PLY56_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21776.1"
                     /translation="MRLQGVSTPAVSYGHVNALGSELLFPEGGEVAHTHIYSSLGEGI
                     RRLREPVRWSEAGMAPFFLEFRERLSQAFLGEPVGFSFGAEGPITTAYELRGEEFFME
                     LLDSPSLAGEFLGAVVESTLEFHRWVCALDGRNPVNPNAGGMCDDLASFIPARLFREM
                     VLPFWEQYYRGTTTGRRHAHVEDLRAEQLPFLEEIGLSFFDPSISPKLTPRIVRDHCR
                     VPFTWRLGSFHYREMDCQEVEDFVYQAVADGASGVHTILAETMCAPEVVEKVRAFIRA
                     AKETERLLSEGATREEIGALVSPAGREKLWDGWCGYLGPKSSRGGRSDGIGHR"
     gene            2133..2600
                     /locus_tag="PLY56_09605"
     CDS             2133..2600
                     /locus_tag="PLY56_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ21777.1"
                     /translation="MALVIDESWYRKPAGIREEDSAGGVVVRLEQGRLLVALVLEGDH
                     QLCVLPKGHVEPGESFEEAARREIAEEAGLTDLTFLGELGTRERLDFARKEWKRTHYF
                     LYLTRQAHGTPTDRSHAYTLRWVPLDADLPLFWPEQAELIRSNRERIRQLAGA"
     gene            2743..>4001
                     /locus_tag="PLY56_09610"
     CDS             2743..>4001
                     /locus_tag="PLY56_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21778.1"
                     /translation="MHLWLLAGSIWITAATMVSGAPAGAREAPLLAGYAGAFFGSDSY
                     KRYLPLLRDHGFNTVDLKVHPADFDMNSPEMEGFITEVVREARAHGLRVHLYLYPEKK
                     GQRDPATATAGPYVGDDGTVHERMYCLYQEEAWLSMFRRVFYLARLSRTLPIEAVKID
                     IEININNNPCYCDDCWRTFDGASGGRDLPASRRKELLRWSGKEKAYLAHLESRLDRAV
                     IAFRERAHRENPRLSLGMMPARDDIFSRAFARHLATKEAPAYLDNWIPYDGLGWRDEV
                     AAARDRMKALNPHNRFIPWFRINRYRPGDLPAHAFALATQTDGYNLWTLSMIHPEEPP
                     RGPGYALPPEHRDAMAYWRAFGEANAAVRQWLARPEDYQPPFALKPLQPLAPGLDLEG
                     IAILALKPLGPRDTRRPVPSPSTYRGRT"
BASE COUNT          679 a         1414 c         1276 g          622 t
ORIGIN      
        1 ggatcagcgc gtggagaata tgcgcgccat cggcgacaaa cgccaccgcg aagccggccc
       61 acatcactgg gttgctgaag aacgcgggca cgccccgccc gcccggctcc gtcatctcca
      121 gggggagctg gaccagggga aacgccagtt tctccttctc cacccagggc cggtgcatca
      181 gcgccaccag gcacgccatc gccagccaga gcgccaggaa gaagcccacc cacgcgagca
      241 cgggaaccac ccacgcgccc cacggaatcg gggcgtggcg caacccctcg tagaacccgc
      301 gtaccacctc cgggtcgcgc ggcgtcgccc aggccgggat gagcgggtgt gtctcgccca
      361 ggcggttgcc cgcgccggcg aagtagaacg gcgccgtgat gaacgccagg aggatgcgca
      421 ccatcccgca gttgtagggc gtggttgcca ccgtgaccat gaagaaggtg gcaatcagct
      481 ctccccgcga gaacgcgtgc cgggagccgc ggaggaagct gtttatcagg agcagcaaga
      541 ggatggagcc gacggcgggg atcgtcggca cgctctcgct gatgaagacg cccaccgcct
      601 ccagctccga ctcgcgcacc caaaaccccg tcaggaacgt gacgatcagg ctgatcagaa
      661 cggagcgaac cgtgatcgcc cggcccgcgc ccgccagggg cgcgacctct tccactctct
      721 cgcgcaaggc cgccatgtgg ataggccccc ctcacgtcgg tgattgcggt aggggggtgt
      781 tcccctccca acgcccgatt cctgctcccc cggagattcc gcgcccggaa ggaggcttct
      841 cccgcccggc gaacgaacga tctgtgctgt gaagcggccc agggcggcga ccgcccttcc
      901 cgaaaggatg cgatgacaac gcgaccagat tacatgcgcg cccgcgacga ggcggggttc
      961 cgctggcatg tctcggcgag ccagggcgct ttgcgcgagg tgaccggcat tccaatccga
     1021 gagttcaacc tgaacccgga agcgtgcatc gaggcgtatc gccgtggccg gcccctgctg
     1081 cgcgagctgt tcggccccga ggtgcgcctn nnnnnnnnng cagctggcct aatacgttgc
     1141 tcaggagaac ggcggccccg gggtgcgcct tcagggcgtc tccacgccgg cggtcagcta
     1201 cggacacgtg aacgccctgg gaagcgagct cctctttccc gagggtggtg aggtcgcgca
     1261 cacgcacatc tactcctcgc tgggcgaggg aatccgtcgg ctgcgcgagc cggtgcgctg
     1321 gtccgaggcg ggcatggcac cgttcttcct ggagtttcgc gagcgcctga gccaggcatt
     1381 cctcggcgag ccggttggct tctccttcgg cgccgagggc cccatcacca ccgcttacga
     1441 gctgcgcggc gaagaattct tcatggagtt gctcgacagt ccctcactcg cgggcgagtt
     1501 tctcggagcg gtggtcgaaa gcacgcttga attccaccgc tgggtctgcg cgctcgatgg
     1561 ccgcaacccg gtcaatccaa acgcgggcgg aatgtgcgac gacctggcga gcttcatccc
     1621 cgcgcgcctc ttccgcgaaa tggtccttcc cttctgggaa cagtattatc gcgggaccac
     1681 gaccggccgg cgccacgccc acgtcgagga cctgcgcgcc gagcagctcc ccttcctgga
     1741 ggagatcggg ctcagcttct tcgacccgtc gatctcgccg aagctcaccc ccaggatcgt
     1801 gcgcgaccac tgccgcgtcc cgttcacctg gcggctcggc tccttccatt accgcgaaat
     1861 ggactgccag gaagtcgaag atttcgtcta tcaggcggtc gccgatggcg cgagcggcgt
     1921 ccacaccatc ctcgccgaga cgatgtgcgc gcccgaggtc gtcgaaaaag tgcgcgcgtt
     1981 catccgcgcc gccaaggaga ccgagcgcct cttgagcgag ggtgccacgc gcgaggagat
     2041 cggcgcgctc gtctctcccg ccgggcgcga gaagctgtgg gacggctggt gcggctacct
     2101 cggccccaag agcagccgcg gtggcaggag cgatggcatt ggtcatcgat gagagctggt
     2161 accgcaagcc cgcggggatc cgcgaggagg attcggccgg cggggtcgtc gtccgcctgg
     2221 agcagggccg cctcctggtc gcccttgttc tggagggcga ccaccagttg tgcgtcctcc
     2281 ccaaaggcca tgtcgagcct ggagagagct tcgaggaggc cgcccggcgc gagatcgcgg
     2341 aggaggcggg gctcaccgat ttgaccttcc tgggagaact cgggacgcgc gagcgcctcg
     2401 atttcgcccg caaagagtgg aagcgcaccc actatttcct ctatctcacc cggcaggcgc
     2461 acggcacgcc caccgacaga tcgcacgcct acaccctgcg ctgggtcccc ctcgatgcgg
     2521 accttcccct cttctggccg gaacaggccg agttgatccg ctccaaccgc gagcgaatcc
     2581 gccagctggc gggtgcctga gagcgatcgc tccaaacccc acccgtggtg agaaggaggc
     2641 cgccctctcc acggacaacc tgtcccccgg gtacccggtg cgtcgcaaca aagcgcgccg
     2701 ttcattgcag gcaggcaacg catcgatggg aaggagttcg ctgtgcatct gtggctgctc
     2761 gctggctcga tctggattac ggccgcgacc atggtaagtg gcgcgcccgc cggcgctcgc
     2821 gaagcccctc tccttgccgg ctacgcgggc gctttcttcg ggagtgacag ctacaagcgc
     2881 tatctgccgc tgctgcgcga ccacggcttc aacaccgtgg acctgaaagt ccatccggcg
     2941 gacttcgata tgaacagccc cgagatggag ggtttcatca ccgaggtggt ccgcgaagca
     3001 cgcgcgcacg ggttgcgcgt tcacctctac ctctatcccg agaagaaggg acaacgcgac
     3061 ccggcaacgg caaccgcggg gccgtacgtg ggcgacgacg gcaccgtgca cgagcggatg
     3121 tactgcctct accaggagga agcctggctc tccatgttcc ggcgtgtctt ctacctggcc
     3181 cggctctcgc gcaccctccc catcgaggca gtgaagatcg acatcgagat caatatcaac
     3241 aacaatccgt gctactgcga cgactgctgg cgcaccttcg acggcgcctc cggcgggcgc
     3301 gacctgccgg cctcgcgccg gaaggagctg ttgcgctggt ccggcaagga gaaggcgtat
     3361 ctcgcacacc tcgaaagccg gctcgaccgc gccgtgatcg ccttccgcga gcgtgcccat
     3421 cgcgagaacc cccgcctctc cctgggaatg atgcccgcgc gagatgatat cttctcgcgc
     3481 gcgtttgccc gccacctggc gaccaaggag gcccctgcct acctcgacaa ctggatcccc
     3541 tacgacggtc tgggctggcg cgatgaagtc gctgccgcgc gcgaccgcat gaaggcgctc
     3601 aacccgcaca atcgtttcat cccctggttt cggatcaacc gctaccgacc gggggacctg
     3661 ccggcgcatg ccttcgccct ggccacgcaa acggatggct acaacctctg gacgcttagc
     3721 atgatccacc cggaggagcc cccgcgcggg cctggctacg ctctgccccc ggagcatcgc
     3781 gacgcgatgg cctattggcg tgccttcggc gaggcgaacg ccgccgtccg gcagtggctc
     3841 gcgcgcccgg aggactacca gccccccttc gcgctgaagc cactccagcc gctggcaccc
     3901 gggctcgacc tggaggggat cgccattctg gcgctcaaac ccctcggccc ccgagatact
     3961 cgccgcccgg ttccgtcacc cagcacctat cgcgggcgca c
//
LOCUS       DAOSCV010000357         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14210_length_4000_cov_3.081369, whole genome shotgun sequence.
ACCESSION   DAOSCV010000357 DAOSCV010000000
VERSION     DAOSCV010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14210_length_4000_cov_3.081369"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLY56_09615"
     CDS             <1..988
                     /locus_tag="PLY56_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOJ21779.1"
                     /translation="YLDRTSAFTLAAGALALRDSGYSVAAGEGLRAGVTMGTAYGCLG
                     TMGAFWEGVLEIGPRLSSSLLFTHSYINTPVSLASIEFNLAGPHGCFCEGAASAGHAL
                     AEAATQLALGRADAMLAGGGESLSTYLYRGYAGMGWLSPGEGGSEGMRPFAASRNGCV
                     LGEGAGFLLLEREEAARQRGARPRARLLGVGLGGGLAGGGAAAAMRAALAEAGVTPAE
                     VDALFASAAGMREADAAEAAGIEEVFGGRCVPVVTLKAALGETLGASGALAALAAVHA
                     LETGLLPGAPNVGESEFSLDLVREPRTATCHYILVNAFDPRGGCVSLLFGRA"
     gene            1460..2263
                     /locus_tag="PLY56_09620"
     CDS             1460..2263
                     /locus_tag="PLY56_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21780.1"
                     /translation="MSRSGGTLQGYFLTPQGQFGPRTFTLVERPGKARESVHPVPSAY
                     YLSIVAKSTTAPIARAEVLTLRNRHPLLSVRMDAPAAEVRLPQGEAAWLGVRPEEGLA
                     FRVVLHLVDGQRVDARIQPDGIRLLRVPPLRVNWLDFVRIEETRRMLRAVGDDVLPGF
                     RADDVPFALLGEEGQCVLVDHPRPPRGFRRYRGPCPVAGRVYVGQMRRENPTPHSSEI
                     EGICTALLPYDPSWFALPMAPGVKRNEREAAWCFEAILHEACTWSGCGG"
     gene            2260..3570
                     /locus_tag="PLY56_09625"
     CDS             2260..3570
                     /locus_tag="PLY56_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21781.1"
                     /translation="MKDDSSPEIEISPRAAAIEEAELDVTCEAIEHLDGDREKLLALV
                     RDYVALRECRRGIELPLPTTELREAIEGLAYFAAWRAAEVARGRYDPILLRQADPFLK
                     ELEVEDPIIRLEPEVRGIDLPELHLSNYLFYQGACQARLLHALDPQALAAAWRNGALL
                     WRALSRVSGYEMLPAGERERLKQEAMARWDCADQDATLREIQRQGEAEQWRAALAARQ
                     GEGVVLRFRVQFLPGSSGCSEAISSWRFSPVYALSAGDFAIACNQPCLVRSLLAGEEA
                     WMELQTVLPREGFLTLRRDGRFTRLQAGEVVIQADQVRVVGPPSSPVIQIDCLRESDR
                     TRVIHGREGAKGSTRSAVMPFPPESCAASGEAPLAWPEVTATVSGLFLNMATGQQRIR
                     ALDLLVERRMMRGPAATGSRGRARRRLPAPGSRDPARAPRFPRG"
     gene            complement(3482..>4000)
                     /locus_tag="PLY56_09630"
     CDS             complement(3482..>4000)
                     /locus_tag="PLY56_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ21782.1"
                     /translation="IAAGLHHLDRAVQLAPTMALNAYHLGLLAMRAGETERAVAAFRR
                     ALEWDPNSPPAWRQLGSLFERTRPEETLRAARRLLEIEQSPYGRIRPLGEHVDVNYAF
                     GHYWMAEHLRHSGDAVAARAEYEKAREILVRYRESLQGIAGLTPEGTAAREPDPEIQA
                     LEAAVSRALATR"
BASE COUNT          553 a         1351 c         1425 g          671 t
ORIGIN      
        1 ctacctcgac cggacctcgg cgtttaccct ggccgcgggc gcgctggcgc tgcgcgactc
       61 cgggtactcg gtggcggccg gcgaggggct ccgcgccggt gttacgatgg gcacggccta
      121 cggctgcctc ggcacgatgg gcgctttctg ggagggcgtc ctggagatag gcccgcggct
      181 ctccagctcg ctgctcttca cgcactccta catcaacacg cctgtcagcc tggcgtcgat
      241 cgagttcaac ctggccggcc cgcatgggtg cttctgcgag ggcgccgctt ccgccgggca
      301 cgccctggcg gaggccgcga cgcagctcgc gctcgggcgc gcggacgcga tgctcgcggg
      361 cggcggcgag tcgctctcaa cctatctcta tcgcggctac gcggggatgg gctggctctc
      421 tccaggcgag gggggcagcg agggcatgcg gcccttcgcg gcctcgcgca acgggtgcgt
      481 cctgggcgaa ggcgccgggt ttctgcttct ggagcgcgag gaggcggcgc ggcagagggg
      541 agcccgcccg cgggcgcggc tgctgggagt cggtctcggc ggcgggctcg ccgggggcgg
      601 cgctgccgcc gcgatgcgcg cggccctcgc ggaagcgggc gtaacacctg cggaggtgga
      661 cgcgctcttc gcctcggcgg cagggatgcg agaggccgat gccgcggaag cggccggcat
      721 cgaagaggtg ttcggcggcc ggtgcgtgcc tgtcgtcacc ttgaaggcgg ctctgggcga
      781 gacgctgggc gcctcggggg cgctcgcggc gctcgcggcg gtccatgctc tggagaccgg
      841 cctcctgccc ggcgcgccca acgttggcga gtccgagttc tcgcttgacc tggtgcgcga
      901 gccacgaacc gccacctgcc actacatcct ggtgaacgcc ttcgaccccc gtgggggctg
      961 cgtctccctc ctctttggcc gcgcctagcc gaaccgcgtc gcgagaatcg cccggccgtc
     1021 tccgccgcgc ctccggctgg cggtagagag agccctgggt atgcgcccgg gttccccggg
     1081 cgcgtccagc gtggggtcgc tccccttcct tgggaccggc gcggggccgg ggactagggc
     1141 gcccggggcg aggcgcgcga tccggcgaac cgcgtccgat cgcgcttccc acgaccggcc
     1201 ttcgccctgg cgatcctgtc cattcttact cagccggcgt gccgtccgag cagtggccag
     1261 gctgcttcgg ctcgcctagc ccgcattatt catcagaata atgaaaagaa gtctctctac
     1321 caggcgtggc gcgggttcgt gggcactgcc ctcgaaaaca ctctgccacc gggcacttgt
     1381 ggcagcaacg gcgcgccacc aggtaaaagt cttttcttga agtattctgg tgaatacttc
     1441 gggctagagg gccacggcgg tgtctcggtc gggggggacg cttcagggct acttcctcac
     1501 accgcagggc cagtttggcc cgcgcacctt caccctcgtc gagcgcccgg ggaaggcccg
     1561 ggagtccgtg catccggtgc cttccgcgta ctacctctct atcgttgcca agtcgacaac
     1621 cgctcccatc gccagagccg aggtactcac gctccggaat cgccacccgc ttctctccgt
     1681 gcggatggat gctcccgccg cggaggtgcg cctgccccag ggggaagccg cgtggctggg
     1741 agtgcgccca gaagagggcc tggctttccg ggtggtcctg catctcgtcg acggccagag
     1801 agtggatgct cgcatccagc ccgacggcat ccgcttgctg cgcgtgcctc ccttgcgtgt
     1861 gaactggctg gacttcgtgc gcatcgagga gacgcggcgc atgctgcgcg cggtcggcga
     1921 cgacgtcttg cccggtttcc gggccgatga cgtgccgttc gcgctcctgg gcgaagaagg
     1981 gcaatgcgtg ctggtcgacc atccccgccc tccgcgcggg ttccggcgct atcggggccc
     2041 ctgccctgtg gcagggcgcg tctatgtcgg ccagatgcga cgagagaatc ccacccctca
     2101 ctcatcggag atcgagggga tctgcaccgc cctgctcccc tacgaccctt cgtggtttgc
     2161 cttgccgatg gcgcccggcg taaagcggaa cgagcgtgaa gccgcctggt gctttgaggc
     2221 catcctccac gaggcctgca cgtggtctgg atgcggcggg tgaaggatga ctcatcaccc
     2281 gagatagaaa tctccccccg tgcggctgct atcgaggaag ccgagctcga tgtcacctgt
     2341 gaggcaatcg agcatttgga tggcgaccgc gaaaagctgc tggcgctcgt gcgcgattac
     2401 gtggcgctcc gcgaatgccg ccgcggcatc gagctaccct tgccgaccac cgaactccgc
     2461 gaagccatcg aggggctggc ttacttcgcg gcctggcgcg ctgccgaggt ggcacgcggg
     2521 aggtacgatc ccatcctgtt gcgccaggcg gatcccttcc tgaaggagct ggaagtcgaa
     2581 gatcccatca tccgcctgga gccggaggtg cgcggcatcg acctgcccga gctgcatctc
     2641 tccaactacc tcttctatca gggtgcctgc caggcacgac tgctccatgc cctcgatccc
     2701 caggcgctgg cggcggcgtg gcggaatggc gctctcctgt ggcgagcgct cagccgcgtc
     2761 tcgggctatg agatgctgcc tgccggcgag cgggaacggc tgaaacagga agcgatggcg
     2821 cgctgggact gcgccgatca ggatgcgacg ctgcgtgaga tccagcggca gggcgaagca
     2881 gagcaatggc gggccgcgct ggcggcccgg caaggcgagg gcgtggtcct gcgattccgg
     2941 gtgcagtttc tgccgggcag ctcgggatgt tcggaggcca tctcatcgtg gcgcttctct
     3001 ccggtctacg ccctctccgc aggtgacttc gcgatagcgt gcaaccagcc ttgcctcgtg
     3061 cgctcgctcc tggccgggga ggaagcctgg atggagctgc aaacggtgtt gcccagagag
     3121 ggcttcctga ctctccgcag agacggtcgg ttcacccggc ttcaggcggg agaggtggtg
     3181 attcaggcag atcaggtgcg cgtcgtgggg ccaccctcct cacctgtgat ccagatagac
     3241 tgccttcgcg agagcgatag gacgcgcgtg atccatggga gggagggcgc gaaggggtca
     3301 acaaggtctg ccgtgatgcc gttccccccg gagtcatgcg ccgcctcagg ggaagcgccg
     3361 ctcgcgtggc cggaggtgac cgccacggtc agcgggctct tcctgaatat ggccaccggg
     3421 cagcagcgga tccgggcgtt ggacctcctg gtcgaaaggc ggatgatgag agggccggca
     3481 gctaccgggt cgcgagggcg cgcgagacgg cggcttccag cgcctggatc tcgggatccg
     3541 gctcgcgcgc cgcggttccc tcgggggtga ggcccgcgat cccctgcaac gactcccggt
     3601 atcggaccag aatctcgcgc gccttctcat actccgcgcg cgcggccacg gcatcgccgg
     3661 agtggcgcag gtgctcggcc atccagtagt ggccgaaggc gtagttcaca tcgacgtgct
     3721 cgccgagggg gcgaatccgg ccatatgggc tctgctcgat ctccaggagg cgccgcgcgg
     3781 cgcgcagcgt ctcttccggg cgcgtccgct cgaagagcga tccgagctgc cgccaggcgg
     3841 gcgggctgtt tgggtcccac tccagggcgc gccggaaggc ggccaccgcg cgctccgtct
     3901 cccccgcgcg catcgccaga aggcccaggt ggtaggcgtt gagcgccatc gtcggcgcaa
     3961 gctgcaccgc gcgatccagg tggtgtagcc ccgcggcgat
//
LOCUS       DAOSCV010000358         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14265_length_3989_cov_2.806050, whole genome shotgun sequence.
ACCESSION   DAOSCV010000358 DAOSCV010000000
VERSION     DAOSCV010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14265_length_3989_cov_2.806050"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PLY56_09635"
     CDS             <1..895
                     /locus_tag="PLY56_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ21783.1"
                     /translation="AQYYATRAQRTVGQRMRTIVEGLPDQASAPGAHGVGDYFPMVTA
                     RLQETSYFHRVEKRLIAAGVPLRAAEFVTICTLVGAAGFLTAAVWSHQPVRALIWGAV
                     GYASPHLVLNALAERRRQILEAQLADTLVMISSALQAGFSFLQGVQMAADQMPQPIGG
                     ELQRMVRLVQLGMPLDEALNQLGDRVQSCDYDLMIAATTLQLATGGNLIRLFKTITET
                     IQERLRIRGEIQATTAEGRLSAMVLVALPVIMLVVLMVIKPDYAHSMLFHPTGVAMLK
                     GAGLLQIVGILAIRRILSLDL"
     gene            1010..1936
                     /locus_tag="PLY56_09640"
     CDS             1010..1936
                     /locus_tag="PLY56_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ21784.1"
                     /translation="MWIAVLIAWTVTLAVGLFFLVTRTDRDIALGRLEEVTEGEIARI
                     AREERARDLSGSPARRLMETLFAPIGEFVKRISSIGGDVEGRLIGAGLTGSLTVGEFV
                     GIKVVALLVLGATALIALPYVRAMGEQSLLLALFALPLAGFVGPDLVLNYLVRARKRM
                     IRKRLVDALDLIVLSMEAGVGFEGALARTAEKIRGPISEELSQMLGEINHGKARADAF
                     SDMAARVQLAELSLLVAAINQADRLGTGIADSLRGLASNLRERNIQIAREAAAKLSVK
                     MVFPLVLFIFPALFIVILGPAIISVIESGGLF"
     gene            2000..2497
                     /locus_tag="PLY56_09645"
     CDS             2000..2497
                     /locus_tag="PLY56_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ21785.1"
                     /translation="MMLHVLCAFAPAWKCAPRGAIRCRQGDERGGSLVELAIVLSVLL
                     LLLLGCGELSGIYKDSLVLCQAAREGARIAAVGATDSAIAARVRSAAPTLDPGRITVE
                     TRFSADDGATFHGVPGVSDGRNDIPAGALIRVRATYPRRLATRVVLPGWTEVTLRCNM
                     IIQRQ"
     gene            2470..2934
                     /locus_tag="PLY56_09650"
     CDS             2470..2934
                     /locus_tag="PLY56_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ21786.1"
                     /translation="MQHDHPAAVGRRRRERGAALVECALVVPFLALLVLGTVEIGRLT
                     RSQLALAQAAREGAQSAARGYSIERCAARVLNTAEHSGLIRANVHTITLEWSTDGGTW
                     ETLANSPSGTENASRRGHLVRVRVRYRHPLISRLVFPGSQREMSQVMVARRQ"
     gene            3178..3660
                     /locus_tag="PLY56_09655"
     CDS             3178..3660
                     /locus_tag="PLY56_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21787.1"
                     /translation="MVILAIMPLVLLTIAILGLTGIIQVEFMRDWSRDMRRALLAGTI
                     AVGSVLYLYHGSYCEVVSPDGHYRASIRYNGLLLRAGMEDPEVVIEGAGRDGLYVVAR
                     QKLDATNGWPQRLVWHPDGRRVACVQQQQGMPPYCRVVRISPHVAVSWYQRVESLERP
                     "
     gene            complement(3717..>3989)
                     /locus_tag="PLY56_09660"
     CDS             complement(3717..>3989)
                     /locus_tag="PLY56_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ21788.1"
                     /translation="HFNAQSPQFVASLITKSEEAYARFFRIVNAPFVGNGFTRWVDGQ
                     YALRRPELGLSNWQFGRLFGRGGILTGDGVYTIRLRVTQTDPNVHR"
BASE COUNT          632 a         1221 c         1407 g          729 t
ORIGIN      
        1 ggcccagtat tacgccacgc gtgcccagcg gaccgtaggg cagcgcatgc gcaccattgt
       61 cgagggcctc ccggatcagg caagcgcccc gggtgctcac ggggtgggtg actacttccc
      121 catggtgact gcgcgattgc aggagacctc ctacttccac cgggtggaaa agcggctgat
      181 agcggctgga gtgccactga gagcggcgga gtttgtcacg atctgcactc tggtgggagc
      241 cgccgggttt ctgactgcgg ccgtttggag ccaccagccg gtgcgcgcgc tgatctgggg
      301 cgcggtcggg tatgcatcgc cacacctggt actcaatgcc ctggcagagc gccgccgcca
      361 gattctggaa gctcagctcg ccgacacgct ggtgatgatc tcttcggcgc tgcaggcagg
      421 cttcagcttc ttgcagggcg ttcagatggc cgcggatcag atgccccagc cgattggcgg
      481 cgagttgcag cgcatggtgc gcctggtgca gcttggcatg cccctggatg aggcgctgaa
      541 ccaactgggc gaccgcgttc aaagctgtga ctacgacctg atgatcgcgg ccacgacgct
      601 gcaactggcg accggcggca acctgatccg tctcttcaag acgatcacgg agacgattca
      661 ggagcgcctg cgcattcgcg gcgagatcca ggcaacgacc gctgagggac ggttgagcgc
      721 catggtcctc gtcgccctcc cggtgatcat gctggtggtc ctgatggtga tcaagccgga
      781 ctatgcccac tcgatgctct tccacccgac gggcgttgcc atgctcaagg gtgcggggct
      841 cctccagatc gtcggcattc tggccattcg gcggatattg agcctggacc tgtagcccgg
      901 caacacgccg tgggtagcgg caagccgggg agcgtgaagc aaaggacggg gccgcgcgtt
      961 gggagcggcc aggtttcttc gtcaccaact tgggctgggg agatagacaa tgtggatcgc
     1021 ggtactcatc gcctggacag tgacgctcgc cgtgggcctc ttcttcctcg tgacgcgcac
     1081 cgatcgcgac atcgccctgg gccggctgga ggaagtgacc gaaggcgaga ttgctcgcat
     1141 cgcgcgggaa gagcgcgcgc gcgatctgag tggctctccc gcgcgccggc tgatggagac
     1201 gcttttcgcg cccatcggcg agtttgtcaa gcgcatctcc agcatcggcg gtgatgtgga
     1261 gggacgcctc atcggcgctg ggctcaccgg atcgctcacc gtcggcgagt tcgtggggat
     1321 caaggtggtg gcgcttctgg tgctgggcgc cacggcgctg attgccttgc cgtacgtgcg
     1381 cgcgatgggc gagcagtcgc ttctcctcgc gctgttcgcg ctaccgctgg ccggcttcgt
     1441 ggggccagac ctggtgctca actacctggt gcgcgcgcgc aagcgcatga tccggaagcg
     1501 attggtggat gcactcgacc tgatcgtgct gagcatggag gcgggtgtgg gcttcgaggg
     1561 cgcgctggcg cgcacggcgg agaagatccg cgggccgatc tccgaggagc tcagccagat
     1621 gctgggagag atcaaccatg ggaaggcgcg tgccgatgcc ttcagcgata tggcggcccg
     1681 ggttcaactc gcggaactct ctctcctcgt tgccgccatc aaccaggcgg accggcttgg
     1741 cacggggatc gccgattcgc tgaggggcct ggccagcaac ctgcgcgagc gcaacatcca
     1801 gatcgctcgc gaggcggcgg ccaaactctc ggtgaagatg gtgtttccgc tggtgctctt
     1861 catcttcccg gcgctcttta tcgtcatcct gggccccgcc atcatctccg tgatagagtc
     1921 cggggggctg ttctgagagt ctgggcagcg tgctcgccgt cgccggccac ggcgagcatt
     1981 ggtgacacat gagggagcga tgatgctgca cgtactctgc gcttttgcac ccgcatggaa
     2041 atgcgcgccg cgcggcgcaa tccgctgccg ccagggagat gagcggggcg gaagcctggt
     2101 cgagctcgcc atcgtgttga gtgtgctctt gctgttgctc ctcggatgcg gcgagttgag
     2161 cggtatctac aaggattccc tggtgctctg tcaggcggcg cgcgagggcg cgcgcatcgc
     2221 cgccgtcggc gcgacagaca gtgcgattgc agctcgggtg cgctcggcgg cgcccaccct
     2281 ggacccgggt cggattaccg tcgagacgcg ttttagtgcc gatgacggcg cgaccttcca
     2341 cggagtgccg ggggtatcgg atgggcgcaa cgatattccc gccggcgcac tcatccgggt
     2401 gcgtgccacc tacccacggc ggctcgccac ccgggtagtg ctcccggggt ggacggaggt
     2461 aacgctgcga tgcaacatga tcatccagcg gcagtaggac ggcgacggcg cgaacgtggc
     2521 gccgcgctgg tagagtgcgc tttggtggtg ccttttctcg cgcttctcgt cctgggaacg
     2581 gttgagatcg ggcggctcac gcggagccag ctggccctgg cgcaggccgc tcgggaaggc
     2641 gcccagtcgg ccgcgcgggg atattccatc gagcggtgcg cggcccgcgt gctgaatacg
     2701 gcggagcaca gtgggttgat ccgcgccaat gtgcacacga ttactttaga gtggagcacc
     2761 gatggtggta cctgggagac gctcgcgaat agcccgagcg gcacggagaa cgcttctcgc
     2821 cggggccacc tggtgcgtgt ccgcgtgcgc taccggcacc cgctcatcag ccggctggtc
     2881 ttccccggaa gccagcgcga gatgagccag gtgatggtgg cccgaaggca gtagccacaa
     2941 aggcgggggc ggcgcggtct gccgtgccct ggcggcaccg cgaaccgcgg gaggcacgtg
     3001 cctccgagac gaacggcact agaagacgag ggctcgaacc agccgttcgt ctcccgcccc
     3061 tgatgcaggg ggggacgggt gacatccgcc cctaatcgcg taggacggcc gccttttcag
     3121 agaggagcgg cccatttccg gggctgaatg ccagaagaga gtgggaggga gacgaggatg
     3181 gttattctgg ctatcatgcc gttggttcta ctgacgattg ccattctggg gctcaccggg
     3241 atcatccagg tggagttcat gcgggactgg tcgcgcgata tgaggcgtgc tcttctggcg
     3301 gggacgattg ctgtggggtc agtactctac ctgtaccatg gctcctactg tgaggtcgtc
     3361 tccccggatg gtcattatcg cgccagcatt cggtataacg gcttgctcct gcgcgccggc
     3421 atggaggatc ccgaggtggt gatcgagggc gccggccgtg atggcctcta cgtggtggct
     3481 cgccagaagc tggatgcaac aaacggttgg ccgcagcgcc tggtgtggca tccggatggc
     3541 cggcgcgtcg cgtgcgtgca gcagcagcag ggaatgcctc cctattgccg cgtcgtccgc
     3601 atctctccgc acgtcgctgt ctcgtggtac cagcgcgtgg agagcctgga gagaccatag
     3661 ccgcaggcgg gggcgggcat tggcgcgccg atgcctgccc ctgcccgcag ttcctgttag
     3721 cgatgcacgt tgggatcggt ctgcgtcacg cgcaggcgga tagtgtagac gccatccccg
     3781 gtcagaatgc ccccgcgccc gaaaagccgt ccgaactgcc agttggagag gcccagctcc
     3841 gggcggcgca gcgcgtactg cccgtccacc cagcgggtga acccgttgcc cacgaagggg
     3901 gcgttcacga ttcggaagaa gcgcgcatac gcctcctcgc tcttcgtgat cagcgaggcg
     3961 acgaactgcg ggctctgtgc gttgaagtg
//
LOCUS       DAOSCV010000359         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14266_length_3989_cov_2.470259, whole genome shotgun sequence.
ACCESSION   DAOSCV010000359 DAOSCV010000000
VERSION     DAOSCV010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14266_length_3989_cov_2.470259"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLY56_09665"
     CDS             <1..383
                     /locus_tag="PLY56_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ21789.1"
                     /translation="FQTWIFQIANNVCVDRFRRTRRERIAYSLDEPVETEEGVVEREI
                     PDWSWDPEAVAQSRELQALVQKTLTTLSDKLRPVIILHDLEGMRYEEIAEILNIPLGT
                     VKSRLFNARVELRNRLRPYMETQA"
     gene            465..995
                     /locus_tag="PLY56_09670"
     CDS             465..995
                     /locus_tag="PLY56_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ21790.1"
                     /translation="MKCRRVRKLLTEYADETLSISVEEQVRLHLSSCRRCATLSEELA
                     QVVGMLQALPRLETSPDFAHQLRRHLPVAVALSRRAPARSGFLSWLHLWRPTGTRRLG
                     LVITPVALGLGLALYLFSLTSLGTQKPIANMEPVISEGAYLATIAREHAGYVSEHPLI
                     DSSAANLKAVATATLP"
     gene            1011..2006
                     /locus_tag="PLY56_09675"
     CDS             1011..2006
                     /locus_tag="PLY56_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOJ21791.1"
                     /translation="MRKRLRACVRVWGAIALLMIGALPPCAAIEFGLPVWRVVGAMES
                     AEAIVRRLVELGRQAEFVGTQETTLFLPKRQFSRQRVIQRDGRRKVIYLSPPQLKGKE
                     ILVDGSRILFDDGTGTAAPDIGFGGELTELSMKHLGTAHVAGRKTHVVEIRPPSRASA
                     SRKVWIDAQEWVPLCWEERDAEGNLVSKTRYITIRFGGGVPRIAPPAAASRSLTPPAV
                     PMTPDEAEKLAGFPVHEPRYLPKGYHRQAVSVISLSKGHRGAYSINLVYSNGLDLITL
                     WQTPAALIENAPRPGKPRVSSFGQWVWVRGGYHYTLSATPPLPKAEMHKVIESIP"
     gene            2443..3456
                     /locus_tag="PLY56_09680"
     CDS             2443..3456
                     /locus_tag="PLY56_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOJ21792.1"
                     /translation="MEPIGQTLREARERKGVTLQEAREALRIHSAYLDSLEAEEFDKL
                     PAPAYTRAFIRQYAAYLGLDPEPLVAAYNASAGTETPSLNVGWSAAPAVKSSWSIRAV
                     PAIATTILAVVLLGGVYGAHLFLVGKEEARRALEEAEKTRKTPVARKAPAPAGARGVQ
                     APEAASVATQPGKAAAEAPAKAEPAGLHPAAKPVAPPTSVPATPAVPVQPSGAATPQP
                     APGRQAQGAQGSASGAATLDESSATPAQSGVHTTLRATAPCWVRIVADGKPVFEGTLQ
                     PGTERTWSAGKVIEMTMGNAGGVRLVVDGKELGAAGRPGEVVRRVFRAESPAQVAGSA
                     QVP"
     gene            3527..>3989
                     /locus_tag="PLY56_09685"
     CDS             3527..>3989
                     /locus_tag="PLY56_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21793.1"
                     /translation="MKRVPLSLSRWLTTERLLLLLAPGILVLLSLNKIGDPDLWWHLK
                     AGEWILDHRAIPQNGLWSWAGEEREWLAHEWLFEVLAAGLYRLAGLRALVVAKALLAG
                     VAFLLVARHARVRGGSAGLASVLTLWAGLAALPFITERPQLLTYILTAFFLS"
BASE COUNT          688 a         1242 c         1370 g          689 t
ORIGIN      
        1 ccttccagac ctggatcttt caaatcgcga acaacgtgtg tgtcgaccgt ttccggcgca
       61 cgcggcgcga gcgcatcgcc tactcgctcg atgagcccgt cgagaccgag gaaggcgtcg
      121 tggagcgcga gatccccgat tggtcatggg accccgaagc ggttgcgcaa agccgggagt
      181 tgcaggcact tgttcagaaa acgctgacca cgctctccga caagctgcgt ccggtcatca
      241 tcttgcatga tctcgagggg atgcgttacg aggagattgc cgagatccta aacatccccc
      301 tgggtaccgt caaatcgcgg ctcttcaacg cgcgtgtcga gttgaggaac cggctgcggc
      361 cctatatgga gactcaagca taaccagtgc cctctacgaa aacgcgagag tgggcatacc
      421 cctgcggctt ccaggaacgc gcaggggtgg gaggatgcga agcaatgaag tgtcgcaggg
      481 tacggaagct gctcacggaa tacgcagacg agaccctgag tatcagcgtg gaggagcagg
      541 tgcgcctcca cttgagcagt tgccgtcggt gcgcaacgct gtcagaggag cttgcgcagg
      601 tagtcgggat gctccaggct ctcccgaggc tggagacctc gccagatttc gcgcaccagt
      661 tgcgcaggca tttgccggtg gccgtggcgt tgtcgcggcg cgcgccggcg cggagtggat
      721 tcctctcctg gttacacctg tggcggccta ccgggacgcg acgcctgggc ctggtaatca
      781 cccccgttgc tctaggcctg ggcctagcac tctatctctt cagccttact tctcttggaa
      841 cgcaaaagcc gattgccaac atggagcccg tgatctcgga aggcgcctat ctggccacca
      901 tcgcgcgcga gcacgccggt tatgtcagcg agcatccgct gattgattct tccgccgcga
      961 acctgaaggc cgtcgccacg gccacactgc catagccggg ggagagacgg atgaggaaga
     1021 ggttgcgtgc ttgcgtgagg gtgtggggcg cgatcgcgct gctgatgatc ggcgcactgc
     1081 ctccctgcgc tgcgatcgag ttcggtttgc ccgtctggcg cgtcgtcggc gcgatggaga
     1141 gcgccgaggc gatcgtccgc cgcctggtgg agctgggtcg acaggccgag ttcgtgggta
     1201 ctcaggagac cacgctcttc ctacccaagc gccaattcag ccgtcagagg gtgattcagc
     1261 gggatggacg ccggaaggtg atctacctat cgcctcctca gctcaagggc aaagagatcc
     1321 tcgtggatgg atcgcgaatc ctctttgatg acggcacggg aacagccgcg ccggatatcg
     1381 gtttcggagg ggagctgacg gagctatcga tgaagcattt gggcacggcg cacgtggccg
     1441 gccggaagac gcacgtcgtc gagatccgcc cgccgagccg ggccagtgcc tcgcgcaaag
     1501 tgtggattga tgcgcaggag tgggttcccc tgtgttggga agaacgcgac gcggagggga
     1561 acctggtctc caagacgcgc tacatcacca tccggtttgg tggcggcgtg ccgcgcatcg
     1621 cgccgcccgc ggctgcttcg cgctcgctga cgccgcccgc ggtacccatg acgccggacg
     1681 aggcggagaa gctggccggc tttcccgtcc acgagccgcg ttaccttccg aagggctacc
     1741 atcgtcaggc cgtcagcgtg atctcgctta gcaaggggca tcgcggcgcc tacagcatca
     1801 acctggtcta tagcaatggt ctggacttga tcacgctgtg gcaaacccct gcagctctca
     1861 tcgagaacgc gccccggccg ggcaagcctc gagtcagttc ctttgggcag tgggtatggg
     1921 ttcgaggcgg ctaccactac acgctttccg ccaccccacc gttgcccaag gcagagatgc
     1981 acaaggtgat cgaatcgatc ccgtagcctg ccggggggcc cgcgagggga aagttgcccg
     2041 ggcgctcgta atctggaacg ggttcgcagt gcatcccacc gggtcaggcc cgaagccagc
     2101 cttcagagca ccctgctgct cgccacgcat cggcgccgcg gaaaacgggt acacgaaggc
     2161 gataggctcc ggaggcgata ggctcggtgc cgtggcagga gtcctgggcc ggcgcgggca
     2221 tggtgagagg cgcaggcatc ttccacgggc cgctcggaac agttgccggg aaggcagcgc
     2281 cggcggtgat cgccgggcct gccagggaat ggcgtcctca ttgtgccgga cccttgtgcc
     2341 ttcggcagcc gccttgactt cggagtgcca cccaattata atggccgtgt ggggccagac
     2401 actcagtttt ttcggcgacg ttgaggtgag ggatggagtg agatggagcc gattggtcag
     2461 actctaagag aagcgcgcga gcgcaaggga gtgacactgc aggaggcacg ggaggcgcta
     2521 cgcattcact cggcctatct ggattcgttg gaggccgagg agttcgacaa gctgccagcg
     2581 ccagcctata cccgggcctt catccggcag tacgcggcct acctggggct ggaccccgaa
     2641 ccgttggtcg cagcatacaa cgccagcgcc gggaccgaga cgccgagcct caacgtgggc
     2701 tggagcgcgg cgcccgcggt gaagtcgtcg tggagcatcc gcgcagttcc agcgatcgcc
     2761 acgacgatac tggcggtggt cctcctcggt ggggtttacg gggctcacct ctttctggta
     2821 gggaaggaag aggctcgtcg ggcactggaa gaggccgaga agacgcgcaa gacccccgtg
     2881 gcgcgcaagg ctcctgctcc ggcgggtgcc cgcggcgtgc aggcaccgga ggctgccagc
     2941 gtcgccactc agccaggcaa ggcagctgcg gaagctccgg cgaaggcaga gcctgccggc
     3001 ttgcatcctg ccgcgaaacc ggttgcgccg ccaacgtccg tcccggctac cccagcggtg
     3061 ccggtccagc cgtcaggcgc ggcgactccc cagccagctc ctggtaggca ggcacagggc
     3121 gcgcaaggga gtgcgagcgg ggccgcgacg ttggatgaga gtagcgccac ccctgcgcag
     3181 agcggtgtgc atacgacgct ccgcgccacg gctccgtgtt gggtgcggat tgtggccgat
     3241 ggcaagccgg tcttcgaggg cacgcttcag ccgggaaccg agcgcacctg gagcgccggc
     3301 aaggtgatag agatgacgat gggcaacgcc ggcggcgtcc gcctggtggt ggatggcaag
     3361 gagctggggg cagcgggacg gcccggtgag gtagtgcgcc gagtcttccg ggcagagtcg
     3421 ccagcgcaag tggccggcag cgctcaggta ccgtaacacc aatcggcagg catagctgcg
     3481 gccaatcggt gccgcgcgat tggccgcagc cgccccgtag agcatgatga agagagtccc
     3541 tctatctcta tcacgctggc tgaccaccga gcgcctcctg ctcctcctcg cgcctggcat
     3601 cctggttctc ctctccctca ataagatcgg cgatcccgac ctctggtggc atctcaaggc
     3661 gggcgagtgg atccttgacc atcgggcgat cccgcaaaat ggcctctggt cctgggccgg
     3721 cgaagaacgg gagtggctgg ctcacgagtg gctgttcgaa gttctcgcgg ccggactgta
     3781 tcgcctcgcg gggctgcgcg cgctcgtggt ggccaaggcg ctgctcgccg gcgtggcgtt
     3841 tctcctcgtg gcccggcatg cccgggtgcg tggcggcagc gcggggttgg cctccgttct
     3901 aacgctgtgg gcggggctgg cggcgcttcc cttcatcacc gagcggcccc aactgctgac
     3961 ctacattctc acggcgttct tcctctcgg
//
LOCUS       DAOSCV010000360         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14277_length_3986_cov_3.690155, whole genome shotgun sequence.
ACCESSION   DAOSCV010000360 DAOSCV010000000
VERSION     DAOSCV010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14277_length_3986_cov_3.690155"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLY56_09690"
     CDS             <1..394
                     /locus_tag="PLY56_09690"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ21794.1"
                     /translation="QERGFGRKVLDIIEAHGISFEHAPTGIDSMCVIFADKELDGKTD
                     ELIADLNRALKPDRIGLVHGLALIATVGEGMAFRVGTAARLFTAIANNGVNVRIIAQG
                     SSEINIIIGVEEKDFVPAVQAIYHAFVE"
     gene            746..2080
                     /locus_tag="PLY56_09695"
     CDS             746..2080
                     /locus_tag="PLY56_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21795.1"
                     /translation="MAKLAGLPIAAMAMGVAVSAQAAPAFELTPKVVPLARETSLTLQ
                     AQPGARFEEIEHEVRIYPMEWRAGSDVAGVTTVKVRPEGGALRISHRFADEQEYLVAV
                     FGNDPKTALASFRVYAVADDLFSRRPYRGDLHQHSHHSDGKESPAYVAAYNRKLGMDF
                     MALTDHHKYAPSLEAIKAFEGLPVDLRIFPGEEVHPPDTGVHIVHFGGKFSVNDLFKT
                     PAYQEGVEQIVASLKEFPAGLNPKSYAACLWALEKIREAGGVSIFCHPYWITGNRYNV
                     PEALITRLFAEKPFDAYEVIGGYWLHQTESNALQVARYYEEVAKGNRVPVVGVSDGHG
                     VHRGLNGWYYTVVLAPSPDYADIRDAIRADYAVAVESLPNAPAARPYGPFRLVKYVSF
                     LLREVFPAHDALCAEEGDAMLAYVAGDQDAVTRLKALQGRVAQLYARLWARE"
     gene            complement(2152..3075)
                     /locus_tag="PLY56_09700"
     CDS             complement(2152..3075)
                     /locus_tag="PLY56_09700"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ21796.1"
                     /translation="MTLSRAEMAGVWSATPTPFTESMEIDEAAVERMVAHHLRLGVKG
                     LFVGGTCGEGPWMTDAQRRALLRAIARSAKGRLFIAAQVTDNSAARILDNMRVAAEEG
                     ADIAVIAPPYFLLNATAQTVLALYQRAICESPLPVGIYDRGSHSSVVVPDEALREIYA
                     EEKVVLVKDSSGEPGRREIALATREKRPELCLLNGNEFRCVEYLQAGYDGLLLGGGIF
                     NGYLAGQIMAAVAAGDIDLANRLQQRMNQMMYSVYGGEQIECWLSGQKKLLVEMGIFS
                     TWRSYLDYPLTASCERAIREVIERERDVLFP"
     gene            3334..>3986
                     /locus_tag="PLY56_09705"
     CDS             3334..>3986
                     /locus_tag="PLY56_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21797.1"
                     /translation="MKISFMTFMTPEWTLDEVIAGARRHGYDGVEPRVTANHKHGIEP
                     DMPAEARKEARERFADAGIEIACIATSCTFAATDPAKRQANIDNLKRHIELAADLGAP
                     GIRVFGGARPEGLSMPDAIAIVAEDLSKGCAQACDAGVGIWLETHDHFSRAEDAAAVI
                     EQANCPCLGINWDLMHPFRNGETTAETWGFLKQGAVVRHTHLHDGVEEESKLRLVPIG
                     "
BASE COUNT          699 a         1287 c         1337 g          663 t
ORIGIN      
        1 ccaggagcgc ggcttcgggc gcaaggtgct tgatatcatc gaagcgcacg gcatcagttt
       61 cgagcacgcg cccaccggca tcgactccat gtgtgtcatc ttcgcggaca aggagcttga
      121 cgggaagacc gacgagctga ttgctgacct caaccgcgct ctgaagccgg accggatcgg
      181 gctcgtccac ggactggccc tcatcgcgac ggtgggcgag gggatggcct tccgggtggg
      241 cactgccgcg cgcctcttca ccgccattgc caacaacggc gtcaacgtgc gcatcatcgc
      301 ccagggctcc tcggagatca acatcatcat cggcgtggaa gaaaaggact tcgtgcctgc
      361 ggtccaggcc atctaccatg ctttcgtaga gtaagcggca gcgcagctag gcaagccagg
      421 gagcggcgtt gccatagaac cgctagggga gcgcgccggt gccagaaaca gattgggcgc
      481 cgcgcgctct ccgcgtgtct ctggcaccga gcaaagccac cactccccct gtggcgggcc
      541 gcatccgtga agcaggcgtt cccggaactg gcgggaggag agcgcatgcc tgcgtggaac
      601 catctgccgg gtagggtgcc caccctgccc gaggcaggct cgcgcccggt gtgggaaaca
      661 cgccggaggg gcctgcgctc agagccgctg gcaaccagtc tctggggatg tcacgcggat
      721 ccggccacgg aaaggaacag gacggatggc aaagctggca ggtcttccga tagcggcgat
      781 ggcgatgggc gtcgcggttt cggcgcaggc ggctcctgcg ttcgagctga ctcccaaagt
      841 ggtgcccctg gcgcgcgaga cgagcctcac tctccaggcg cagcccggcg cgcgcttcga
      901 ggagatcgag catgaggtcc gcatctaccc gatggagtgg agagcgggca gcgatgtcgc
      961 aggggtcacc acagtcaaag tacggcctga gggcggagcc ctgcggatct cccatcgctt
     1021 cgccgatgag caagagtacc tggtggcggt gttcgggaac gatcccaaga cagccctggc
     1081 gagcttccgg gtgtatgccg ttgccgatga cctcttctcc cgccggccct ataggggtga
     1141 tctccaccaa cactcccacc actccgacgg caaggagtcg cctgcctacg tcgccgccta
     1201 caaccgcaag ctgggcatgg acttcatggc gctcaccgac caccataagt atgcgccttc
     1261 cctggaggcg atcaaagcgt ttgaaggcct gccggttgac ctgcgcatct tcccgggcga
     1321 ggaggtgcat ccccccgata ccggggtgca catcgtccac ttcggcggca agttcagcgt
     1381 gaacgatctt ttcaagacgc ctgcctacca ggagggggtg gagcagatcg tcgcgtctct
     1441 gaaggagttc ccggctgggc tcaaccccaa gagctacgcg gcctgccttt gggcgctgga
     1501 gaagatccgg gaagccggtg gagtcagcat cttctgccat ccctactgga tcaccgggaa
     1561 ccgctacaac gtgcccgagg cgctcatcac ccgcctcttc gccgagaagc cgttcgatgc
     1621 ctacgaggtg atcggcggct actggctgca tcagacggag tcgaacgccc ttcaggtggc
     1681 gcgttactat gaggaggtcg cgaagggcaa ccgcgtgccc gtcgtaggcg tctcggatgg
     1741 gcacggcgtg catcgcggcc tcaacggctg gtactatacc gtggtcctgg caccctcgcc
     1801 ggactacgcc gacatccggg atgcgatccg cgctgattac gccgtggccg tggaaagcct
     1861 gccgaacgcg ccggccgcgc ggccctatgg tcccttccgc ctggtgaaat acgtcagctt
     1921 cctcctgcgc gaggtctttc ccgctcatga cgccctctgt gccgaggaag gcgatgccat
     1981 gctcgcttat gtcgccgggg atcaggatgc cgtcacccgg ctcaaggcgc tgcaagggcg
     2041 ggtcgcacag ctgtacgcgc ggctctgggc gcgcgaatga gttgcgcccg cggcagggag
     2101 cgcgggcgtg cctcggagtg aggcggcctg gaacccgcgg ggtgatcccg cctatgggaa
     2161 gagcacatca cgctcgcgtt cgatcacctc gcggatagcg cgctcgcacg acgcggtgag
     2221 gggatagtcg aggtagctcc gccacgtgct gaagatcccc atctccacca ggagcttctt
     2281 ctggccggag agccagcact cgatctgctc gccgccgtag accgagtaca tcatctggtt
     2341 catgcgctgc tgcaggcggt tggcgaggtc gatatcgcca gccgccacag ccgccatgat
     2401 ctggccggcc aggtagccgt tgaagatgcc gcctccgagc agtaggccat cgtaacccgc
     2461 ctgaagatac tcgacgcacc ggaactcgtt gccgttgagg aggcaaagct ccgggcgctt
     2521 ctcgcgagtg gcgagagcga tctcacgacg ccctggctcg cccgagctgt ccttcaccag
     2581 gaccaccttc tcctcggcgt agatctccct caaagcctcg tcgggcacca cgacggagga
     2641 gtggctcccc cggtcgtaga ttccaaccgg gagggggctc tcgcagattg cgcgctggta
     2701 gagcgccagg acggtctgag cggtggcgtt gagcaggaag tagggcgggg cgatcacggc
     2761 gatatcggcg ccctcttcag cggccacgcg catgttatcg agaatgcgcg cggcggagtt
     2821 gtccgtcacc tgcgcggcga tgaagagccg acccttcgcg ctgcgcgcga tggcacgcag
     2881 gagcgcgcgc cgctgcgcgt cggtcatcca ggggccctcg ccacacgttc ctccgacgaa
     2941 aagacctttg acccccaggc gcaggtggtg ggccaccatg cgctcgaccg cggcttcatc
     3001 gatctccatc gactccgtga agggcgtcgg cgtcgccgac cacacgcccg ccatctcagc
     3061 tcgcgatagt gtcatctctt ctcctccgct gcgccttacc cggccgtttc cggtgctctt
     3121 cgccacggcg ctggcagttc ctgcgtgcgc tcttctggcg tgatcgcgtg gatgaaggaa
     3181 gcgggtgcgg agcgggggat tcgcgtcgcg ggagcgccat cgctccgctc atgccacgcg
     3241 tggcgagcac gggaacgatg ggcaggagtt gatcggcagg ggggtgaaag agagctccgg
     3301 cgcggacggg cctccgcgcc ggaggagagt gcgatgaaga tcagtttcat gacgttcatg
     3361 actccggagt ggacgctgga cgaggtgatc gctggggcgc gacggcacgg ctatgacggc
     3421 gtcgagccgc gggtgaccgc caaccacaag cacggcattg agccagacat gcccgctgag
     3481 gcgcgcaagg aggcgcgcga gcgctttgcc gatgccggca ttgagatcgc ctgcatcgcc
     3541 acgtcatgca ctttcgcagc gaccgaccct gcgaagcgcc aggcgaatat cgacaacctg
     3601 aagcggcaca tcgagctagc ggccgatctg ggcgcgcccg ggatccgcgt cttcggtggc
     3661 gcccgtcctg aggggctgag catgccggac gccatcgcca tcgttgccga ggacctgagc
     3721 aagggctgcg cccaggcgtg cgacgccggc gtgggaatct ggctggagac gcacgatcac
     3781 ttctcccggg ctgaagatgc cgcagccgtc atcgagcaag ccaattgccc ctgcctggga
     3841 atcaactggg acctgatgca tcctttccgc aatggcgaga caacagccga gacgtggggc
     3901 ttcttgaagc agggagccgt ggtgcgacac acgcacctgc atgacggcgt cgaggaggag
     3961 agcaagctgc gcctcgtgcc gattgg
//
LOCUS       DAOSCV010000361         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14306_length_3981_cov_3.447020, whole genome shotgun sequence.
ACCESSION   DAOSCV010000361 DAOSCV010000000
VERSION     DAOSCV010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14306_length_3981_cov_3.447020"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /gene="argH"
                     /locus_tag="PLY56_09710"
     CDS             <1..852
                     /gene="argH"
                     /locus_tag="PLY56_09710"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ21798.1"
                     /translation="ERDYARFGGVIDRADWMPLGAGALAGTTFPIDRQQVARELGFSR
                     VAPNSLDAVSDRDFIIEFVSASALLMVHLSRLCEELILWTSREFGFCRLDDAFTTGSS
                     MMPQKRNPDLAELIRGKSGRVTGSLVSLLMMVKGLPLAYNKDMQEDKEPLFDAADTVR
                     GCLKVMAAMIQTARFNRDRLAAATRGDFSTATDLADYLVRKGLPFRDAHDVSRRIVMA
                     CLEQGRALEDLSLDELRSYSPLFEEDALLEVSPERSAARRTSEGGTAPERVREQIAQA
                     RELLGGV"
     gene            976..2478
                     /locus_tag="PLY56_09715"
     CDS             976..2478
                     /locus_tag="PLY56_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21799.1"
                     /translation="MATLLSQGMSASLMAPGAGREAAIPIGSRLELMVDDFLIERLEG
                     GARQVLHHPIPREVVLVHDAPWEGSGCNYYSVFCDGDLYRMYYHGWQLGSPKGAADTP
                     HPLVTCYAESRDGIHWEKPSLGLVEYEGSRANNIVLATGELDGVPICAGHIALCKDEN
                     PDAAPEARYKALAVSWELKGLIALGSPDGFHWRPLSKEPVITKGAFDSQNLAFWDSAR
                     GEYRAYVRFFDGGCRGILTATSPDFLHWTEPLPLEYPGTPKEQLYTNQVKPYHRAPHL
                     LLGFPTRYVERGWSPSMEALPELEHRRLRSRACDRYGMALTEGLFMSSRDGRTFRRWG
                     EAFLRPGPERPDTWNYGHQYIAWHPVETAASLPGAANELSLYATESYWTGTYTLLRRY
                     TLRLDGFVSVNAPLSGGAVLTKPIRFEGSRLWLNFSTSAAGAVRVEIQDASGAPIPGF
                     ALADCHETFGDSIERPVVWKRGSDVSALAGRAVRLRFELSDADLYAFRFR"
     gene            complement(2484..>3981)
                     /locus_tag="PLY56_09720"
     CDS             complement(2484..>3981)
                     /locus_tag="PLY56_09720"
                     /inference="COORDINATES: protein motif:HMM:NF037031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HOJ21800.1"
                     /translation="LPTGKVYRYAFAYRTGPGLTGRPMMILDAYTREGEKGSKQLALR
                     RLEPSTEWKRVEGEVRVDEGIVRTRFLLYLYGVGTVWYDDAFFGEAEPGAANRLKNGG
                     FEPPFSSVYDLAPERRGGPVRLVAGFDNAALGKVKQIGPDEFYVYAVPEEGPRSPYLW
                     FHFRVEGCAGREITFHVNPAPFSRESTSGNGTRIPVVSEDGENWTGVAEKSWSEDGSC
                     LTFKHRFTRSPAWICSFFPFTGERVTRFIARNAASPYFTAGVLGKTREGRDLRYYTIT
                     DPAVPEAEKRVLFFTTLQHDLETTGALALEGLCRYLLSDDPGAARLRRSCVTYVVPMM
                     DPDGIAGGNLYCPVGNMNRQWGLGTTPEVGHVEHFAKQLAERGRPIDLFMDFHGWCTP
                     ERSTSFSTFGRELADEATEADALRLVDCIKKRLQGKAYPVLWRKMESFVTSGVIDLRR
                     MAPGWMRFEASARLAYSIEIFGEGECTQQGYFDWGRAFAEGIDEFYAR"
BASE COUNT          646 a         1354 c         1329 g          652 t
ORIGIN      
        1 gagcgcgact atgcccggtt cggtggcgtg atcgaccgtg ccgactggat gcccctcggt
       61 gcaggcgccc tggctggcac caccttcccc atcgaccggc agcaggtcgc ccgggagctc
      121 ggcttctcgc gcgtggcccc caacagcctc gacgccgtct ccgatcgcga cttcatcatc
      181 gagttcgtct ccgccagcgc cctgctgatg gtccacctct cgcgcctctg cgaggagctg
      241 atcctctgga ccagccgcga gttcggcttc tgccggctgg acgacgcctt taccaccggc
      301 tccagcatga tgccccagaa gcgcaatccc gacctggccg agctgatccg cggcaagagc
      361 gggcgcgtca ccggcagcct cgtctccctc ctcatgatgg tgaagggcct gcccctggcc
      421 tacaacaagg atatgcagga ggacaaggag ccgctcttcg acgcggcgga taccgtgcgc
      481 gggtgcctca aggtgatggc cgcgatgatc cagaccgcgc gcttcaatcg tgatcgcctt
      541 gcggcggcca cccggggcga cttctccacg gcgaccgacc tggccgatta cctggtccgc
      601 aagggtctcc cgttccgcga tgcgcacgac gtctcccgcc ggatcgtcat ggcctgccta
      661 gagcagggcc gggcgctgga ggatctctcc ctggacgagc tgcgctccta cagcccgctc
      721 tttgaggagg atgccctcct ggaggtctcg cccgagcgaa gcgccgcccg gcggacgagc
      781 gaggggggca ccgcgcccga gcgcgtccgc gagcagattg ctcaggcgcg agagctcctc
      841 ggcggcgtgt aggttccgcg tccacaccgc gcaccgcgcc ctctcgcggg agcaaagaaa
      901 acctctcccg gcgcaggaag ctgcccggcg gtggcgaaca ccctggcacc gctcgcggca
      961 cggagggaga gggagatggc gacactgctc tcgcagggaa tgagcgcatc gctgatggcg
     1021 ccaggcgccg gcagggaagc tgcgatcccg atcggctcgc gcctggagct gatggtggat
     1081 gacttcctga tcgagcgcct ggagggcggc gcccggcagg tgctgcacca tccaattccg
     1141 cgcgaggtcg tcctggtgca cgacgcgccc tgggaaggga gcgggtgcaa ctactacagc
     1201 gtcttctgcg acggcgacct ctaccgaatg tactaccacg gctggcagct gggctcgccc
     1261 aagggcgccg cggacacgcc gcatccgctg gtcacctgct acgccgagag ccgtgacggc
     1321 atccactggg agaagccgtc gctcggcctg gtggagtacg agggttcgcg ggcgaacaac
     1381 atcgtgctcg cgaccggcga gctcgacggg gtgccgatct gtgccgggca catcgccttg
     1441 tgcaaggatg aaaatcccga cgccgcgccg gaagcacgct acaaggcgct cgcggtctcg
     1501 tgggaactga agggactgat cgcgctgggc tcgcccgacg ggtttcactg gcgtcccctc
     1561 tcgaaggagc cggtgatcac gaagggcgcg ttcgactcgc aaaacctggc gttctgggat
     1621 tcggcgcgcg gtgagtaccg cgcctacgtc cgcttcttcg atggcggctg ccgaggaatc
     1681 ctcacggcaa cgtcgccgga tttcctccat tggaccgaac cattgcccct ggagtatccc
     1741 ggcacgccga aggagcagct ctatacgaac caggtcaagc cgtatcaccg cgcgccgcat
     1801 ctccttctgg gcttccccac gcgctacgtg gagcggggat ggtcgccctc aatggaggcg
     1861 ctgccggagc tggagcaccg ccggctccgc tcgcgcgcat gcgaccggta tggcatggcg
     1921 cttaccgagg gcctcttcat gagcagccgc gacgggcgca ccttccgccg atggggcgag
     1981 gcgtttctgc gccccgggcc cgagcgcccg gatacctgga actacggcca ccagtacatc
     2041 gcctggcatc ccgtcgagac cgccgcgagc ctgcccggcg cggcgaacga actctcgctc
     2101 tatgccaccg agagttattg gaccggcacc tacacgcttc tccggcgcta taccctccgc
     2161 ctggatgggt tcgtttcggt gaacgcgccg ctgagcggcg gcgcggtgct cacgaagccg
     2221 atccggttcg agggaagccg gctgtggctc aacttctcca cctcggcggc tggcgcggtg
     2281 cgcgtcgaga tccaggacgc atccggcgcg ccgattcccg ggtttgccct ggccgattgc
     2341 cacgagacgt tcggcgattc tatcgagcgc ccggtggtgt ggaaacgcgg aagtgacgtg
     2401 agcgccctcg ccggacgggc ggtgcgcctc cgcttcgagc tgagcgatgc cgacctctac
     2461 gccttccggt tccggtgagc gagttaccgg gcgtagaact catcgatgcc ctcggcgaat
     2521 gcccggcccc agtcgaaata gccctgctgg gtacattcgc cctcgccgaa aatctcgatg
     2581 gagtaggcca ggcgggcgct cgcttcgaag cgcatccacc cgggcgccat ccggcgcagg
     2641 tcgatcacgc cggacgtgac gaagctctcc atcttacgcc agaggacggg gtaggctttc
     2701 ccctgcaggc gcttcttgat gcaatcgacc aaccgcaggg catccgcttc ggtcgcctcg
     2761 tccgcgagct ctcgaccgaa ggtcgagaag gaggtggagc gttccggggt gcaccagccg
     2821 tggaagtcca tgaagagatc aatcggacgc ccgcgctccg cgagctgttt cgcgaaatgc
     2881 tccacgtgcc ccacctccgg cgtggtgccc aggccccact ggcggttcat gttccccaca
     2941 gggcaataga ggttgccgcc ggcgatgccg tccgggtcca tcatcggcac gacgtaggtg
     3001 acgcaggagc gccggagccg ggccgcgccg gggtcatccg agagaaggta gcggcagagc
     3061 ccctccaggg cgagcgcccc ggtcgtctcc agatcgtgct ggagcgtcgt gaagaagagg
     3121 acgcgcttct cagcttccgg cacggccggg tctgtgatcg tgtagtagcg aaggtctcgg
     3181 ccctcgcgcg tcttgccaag cacgcccgcg gtgaagtagg ggctggccgc gttccgggcg
     3241 atgaagcgcg tcacgcgctc tcccgtgaag gggaagaagg agcagatcca ggccggcgag
     3301 cgcgtgaagc ggtgcttgaa cgtcaggcag ctcccatcct cgctccatga cttctcagcg
     3361 acgccggtcc agttctcgcc gtcttcgctg acgaccggaa tgcgcgtgcc gttgcccgac
     3421 gtgctctcgc gggagaaagg cgccgggttg acgtggaaag tgatctcgcg cccggcgcat
     3481 ccctctaccc ggaagtggaa ccacaggtag ggagagcgcg ggccctcctc cgggacggca
     3541 tagacgtaga actcatcagg gccgatctgc ttcactttgc ctagcgcggc gttatcgaag
     3601 ccggcaacta ggcgcacggg cccgccgcgc cgctccgggg cgaggtcata cacggaggag
     3661 aaaggcggtt cgaagccgcc gtttttcaac cggttggccg cgcccggttc ggcctcgccg
     3721 aagaaagcgt cgtcgtacca cacagtgccc acgccataga ggtagaggag gaatcgcgtg
     3781 cgcacgatgc cttcgtccac ccggacttcg ccctccacgc gcttccattc cgtggatggc
     3841 tccaaccggc ggagcgcgag ttgcttggat cccttctcac cctcgcgggt gtaggcgtcc
     3901 agaatcatca tgggcctgcc ggtgagccct ggcccggtgc gataggcgaa agcataccgg
     3961 tacaccttgc cggtgggcag g
//
LOCUS       DAOSCV010000362         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14314_length_3980_cov_3.155669, whole genome shotgun sequence.
ACCESSION   DAOSCV010000362 DAOSCV010000000
VERSION     DAOSCV010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14314_length_3980_cov_3.155669"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..757
                     /locus_tag="PLY56_09725"
     CDS             338..757
                     /locus_tag="PLY56_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21801.1"
                     /translation="MSPAAEIALIIIAVLQVGGWVVLAIASFGIYKQLRGLCGKATTL
                     MNQAEDLFGTVRETATVVSERAERISGEVAEKAQHIADLSEQVAERVVQRVDTTSSIV
                     QEAISGPMINLASVRAGVGKGLEVWQALARAKGGNGK"
     gene            754..1149
                     /locus_tag="PLY56_09730"
     CDS             754..1149
                     /locus_tag="PLY56_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOJ21802.1"
                     /translation="MSDSNNSSRDFALGLVIGGLIGAAAALLYAPMAGVEVRRKVGER
                     ATEYAGQAREKVGEFAGCARERAGEAVTTLRERADGLMEQARSRVDELRGRVTAAVDA
                     GVEAAKEKQDELEERIDETAAPMIKPEDA"
     gene            1682..1996
                     /locus_tag="PLY56_09735"
     CDS             1682..1996
                     /locus_tag="PLY56_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HOJ21803.1"
                     /translation="MAERKQRPGPKPGSEGAARIAQAHRGSHEHDREGGFAADPSLAR
                     TAGRLGGERVKEKYGSSFYTEIGRKGGEAVRDTHGTEFYSRIGKLGREARSRRQREEA
                     SE"
     gene            complement(2112..2684)
                     /locus_tag="PLY56_09740"
     CDS             complement(2112..2684)
                     /locus_tag="PLY56_09740"
                     /inference="COORDINATES: protein motif:HMM:NF040140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21804.1"
                     /translation="MEILRIAFEGDKVQITLRMSEPFNTRDKPHLARGLFRMLPRLKK
                     HRCYNEGNLSFQREAASTEIAHLLEHLVLELQAVTLCGRGIRGVTYWNWREEPTGQFH
                     IDIDAPHPGVALGAVWLAQRIIRAIDERHPETIDIETELRTLRAIAKLPVDAIPALPV
                     EDFPRWEATWAQQVAELVARSKEQALVTSH"
     gene            complement(3400..>3980)
                     /locus_tag="PLY56_09745"
     CDS             complement(3400..>3980)
                     /locus_tag="PLY56_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HOJ21805.1"
                     /translation="TETLLLAGVALSALLSAAISFLIALSAREEVVSAVYFWTLGGFY
                     RADWSRVWIALPYAFLGMGLAWLYSRDLNALLLGEECALNLGVDVERTKRVVLAGAAL
                     ASAAAVSVSGIIGFVGLIAPHIVRLIQGPDHRALIPASALTGALFLVICDTLARTLFA
                     PTEMPIGVLTAFTGVPFFLSLLRRSQRVSRHA"
BASE COUNT          746 a         1165 c         1405 g          664 t
ORIGIN      
        1 gtcctctccg gggcccacgg gtggtatctg cccgctccct gttggcgaga tactccacgc
       61 gccggcgtcg ccgcgtggag taatggctgg ctttttttgc ggcggaaacg attagaggcg
      121 cctctccttg tgggatatac ccgcgaggag gggcgccttt tgcatttgtg gccgggggag
      181 cgcaagttcg gatcggctga tcagaggcta gcgcgaagcc ccatagcgtc tgcttggcgc
      241 ccccttgcgg tggggctggg ggcgcgctcg gtgggcgttt gaggggttga cagagagggt
      301 atcatgtgct agaatgctgt aaaaaggggg atacagcatg agcccagcgg cggagattgc
      361 gctgatcatc atagccgtgc tgcaagtggg cggctgggtc gttctggcga ttgcctcctt
      421 tggcatctac aaacagctcc gaggcctctg cggcaaagcc accacgttga tgaaccaggc
      481 ggaagatctc tttggcacgg tcagggagac cgccacggtg gtttccgagc gtgccgagag
      541 aatcagcggc gaggttgcag agaaggctca gcacattgcg gacctcagtg aacaggtggc
      601 ggagcgggta gtacagcggg tggataccac ctcctccatc gtgcaagagg caatttccgg
      661 tccgatgatc aacctggcga gtgtgcgtgc cggggtaggc aagggattgg aagtgtggca
      721 ggcactcgca agagcgaagg gaggcaacgg caagtgagcg atagcaacaa cagcagcagg
      781 gatttcgccc ttgggctagt aattggtggg ttgattggcg ccgccgcagc gctactctat
      841 gcaccgatgg ccggcgtgga agtgcgccgc aaggtggggg agagagcgac ggaatatgca
      901 ggccaggcgc gcgagaaggt gggtgagttt gccggatgcg cgcgcgagcg cgcgggcgag
      961 gcggtcacca cgcttcggga gcgcgcggat ggcctcatgg agcaggcacg ctcgcgcgtt
     1021 gacgagctgc gcggccgggt gacggcagcc gtggacgcgg gcgtcgaggc ggcgaaagag
     1081 aagcaagacg agcttgagga gcgcatcgac gagacggctg ctcctatgat caagcctgaa
     1141 gatgcataaa agtgtctgcc ggcgccaccc cgtggcgccg gcaggcttgc gcgaatcgca
     1201 gtggtacgat gcccggcaag ggcggtgtgc ccccagcgat tggcgtattg atcataccct
     1261 tcacacatcc tcggaagtcc cggctggctc tgcgattgac cggcagcgtc cgggacaccg
     1321 ccggatccgg gaagcggacc gggtctgagc ggggagatgc gccgcagtcg gttctcgttc
     1381 cggcatcacc gccggaaagg ctggggaacg tagtctatgg gggcaaggga agcgtcgtgc
     1441 tgcgaaaccc cgtgggccgg gctccggcaa agggcgcagg gcaggaaacg caacattccg
     1501 aggggagata catcgcgtaa cacggttcgg tggagcgctc gcggcccgca cggtaacgcg
     1561 ccggtatttt gtgtgccggc gccgtggtat ggggtggtgg tccgcgccgc actcaggttc
     1621 tggtgtcggc tcgccgctgt cccatgagtg acgttgtggt atccgaagga ggtcatccaa
     1681 gatggcagag cggaagcaac ggccagggcc caagcccgga tcggaagggg cggctcgtat
     1741 cgcccaggca catcgtggtt cgcatgagca cgaccgcgag ggtgggttcg cggcagaccc
     1801 ctccttggcg cgcacggcgg gccggcttgg cggagagcgc gtgaaggaga agtacggctc
     1861 cagcttctac acggagattg gccgcaaggg cggcgaagcg gttcgcgata cgcacggcac
     1921 agagttttac tcccggattg gcaagctggg gcgcgaggcg cgctcgaggc gccagcgtga
     1981 agaggcaagc gagtaggttc gagaagacga gtgcgttccg gatgcggccc ctcgaacccc
     2041 cgatcccggg ggctcgaggg gccgaatgct ttctgcgctc tccaggctgg ggcgcgggcg
     2101 ctagccgggc ctcagtgtga ggtgacgagg gcttgctcct tggaacgggc gaccaactcg
     2161 gccacctgct gtgcccacgt ggcctcccag cgcgggaaat cctccacggg gagggctgga
     2221 atggcatcca ccgggagctt ggcaatcgcg cgtagcgtgc gcaactcggt ctcgatatcg
     2281 atcgtctcgg ggtgccgctc atcaatcgcg cgaataatgc gctgagcgag ccacaccgcg
     2341 ccgagcgcta cgccgggatg gggcgcgtca atgtcgatat ggaactgtcc ggttggttcc
     2401 tcccgccagt tccagtaggt gacgccccgg atgcctcggc cgcatagcgt cacggcctgc
     2461 aactccaaaa cgagatgctc cagcaggtgg gcgatctcag tggatgctgc ctcgcgctgg
     2521 aagctcaggt tgccctcgtt gtagcaacgg tgcttcttca gccgcggcag catgcggaag
     2581 agcccgcgcg cgagatgagg tttgtcgcgt gtgttgaagg gctctgacat gcgaagcgta
     2641 atctgaacct tgtccccttc gaaagcgatg cgcaggatct ccaagcgtgg tgcctatcct
     2701 tccaacccgt aattcatatc cggccctcac tccactctcg ttgctccggg cgctcggaat
     2761 cctcctcgct tccccggacg cggggcgctc catccagaag accccgaggg cggatactca
     2821 gcactgcgaa tgggtgcgtc cggctctcgg gcagcaacac ctacatcgcg actctggacg
     2881 cctgctcgcg gcaacgccgg tggccccgat agagcgcgaa tttgcagctc gcggcctctc
     2941 ctcgcctggc aatccatcct ttcggattcc ctagcgaggt gcattcgtga aagcggcgcg
     3001 ctggcccgcg gcctctccca ctaggcatta tcggtcgctt gtccctcttc ttaagaagca
     3061 ggcattctgt gcgaccggaa cgaccgaaac gcagcccggc gaccaccgtc gcacaccgga
     3121 taccggtggg ctggcgaaga ccgccatcag gcagatacga cgcggagttc cccaactcct
     3181 tgaggccgca gatcggaacc gcattgaaag cgcaccgtgc ggactaggcg ccgcatcgcg
     3241 cgggccacgg cacgttggat aacgccttcg cgtgcattct aacacaaacg cctccggtca
     3301 cgcaattgtg accgcttgcc tctctcgcag gagaacagct ggatggggcc tggggaatga
     3361 ggcgggaacg gcgtccggtc tggccgggtc cgtcacgtgt caggcgtgcc gggaaacccg
     3421 ttgcgaccgg cgcaggagag agaggaagaa gggcactccg gtgaacgcgg tgagcactcc
     3481 aatgggcatc tcggtggggg caaagagggt gcgcgccagc gtatcacaga tcacaaggaa
     3541 gagggcgccg gtgagcgcgg atgcggggat gagtgcgcga tggtcgggac cttgaatcag
     3601 ccggacgatg tgcggggcaa tcaagcccac aaagccgatg atccccgata cggaaacggc
     3661 ggcggcgctc gcgagggccg cgccggcgag cacaactcgt tttgtgcgtt ccacatcgac
     3721 gcccagattg agcgcgcact cctcgccgag aagcagagcg ttcaggtcgc gcgaatagag
     3781 ccatgccagg cccatgccga ggaacgcgta ggggagggcg atccacaccc ggctccaatc
     3841 cgcgcggtag aagcctccaa gcgtccagaa gtagacggcg ctcaccacct cttcgcgggc
     3901 gctcagggcg atcaggaagg agatcgcggc ggaaaggagc gcgctcaacg cgacgccggc
     3961 aaggaggagt gtttctgtat
//
LOCUS       DAOSCV010000363         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14321_length_3978_cov_3.570482, whole genome shotgun sequence.
ACCESSION   DAOSCV010000363 DAOSCV010000000
VERSION     DAOSCV010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14321_length_3978_cov_3.570482"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLY56_09750"
     CDS             complement(<1..198)
                     /locus_tag="PLY56_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21806.1"
                     /translation="MPATEEKRQEFELPAKELDGIPWRALLIGTLLMPPLTLFGHLSY
                     VIVQSARWTADTLLRGPVVLLF"
     gene            complement(343..1020)
                     /locus_tag="PLY56_09755"
     CDS             complement(343..1020)
                     /locus_tag="PLY56_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ21807.1"
                     /translation="MDEIRADFDRIARLSTRERWDHNNHYHRYLLKHAPPRCELALDI
                     GCGTGEFTRLLAARSRRVLALDLSPEMIRVAREGSAERANIDYHVADVMQFPLEEGAY
                     DCIASIATWHHLPFAEIARKARRALRPGGVLLVLDLFQGTRADRLMSALVVPVNIAMR
                     LVRGQSLRVPAEIRAAWDAHGRHDTYLTLAEVRRLCAAELPGAIIRRHFFWRYSLVWR
                     KPSGGPS"
     gene            1244..1792
                     /locus_tag="PLY56_09760"
     CDS             1244..1792
                     /locus_tag="PLY56_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOJ21808.1"
                     /translation="MGFAVELHFDPQAEMRVRSLWDELARQGIRSLPPLIGSRPHISI
                     AVFDRVDPARVRPELEELAATLAPLPLTFGAVGTFPAKEGVVFLTPVVTPELLELHQS
                     VHARLDALGLRALEHYRPGRWVPHCTVAIDLPREQLLPALEICRSSRVFGSLTLAEIG
                     LVEYRPVRPICSFPLRDPRQEG"
     gene            complement(1814..2797)
                     /locus_tag="PLY56_09765"
     CDS             complement(1814..2797)
                     /locus_tag="PLY56_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ21809.1"
                     /translation="MAGTEEQTSVGEPLDLAALGKTWTPPQANETTPEVSEILAEMPW
                     WAARGLLYIIVAFLLAAGLWAHFSVLDVVAEARGTLLPEGYTRRVEAQTDGVVQFILV
                     REGDRVEANEALIQLDSAEARVRRDNARQEMRTLEEQLLQLRASGASVVEALEKESAL
                     ARLESEIAALDLALARSTIRSPVEGVVTSLEVRAPGAVVKPGDPVATVAPADARLVVE
                     GKMPNQQIAFVRKGLPAKLKFDAYPYQDYGVVNGTVVDVSPDAQSDEKLGSVYKVTVA
                     PDRPVITAYAREFPLRPGMTATIEVVTDRKSVLSLFLAPFKKVQGDLGSAK"
     gene            complement(2831..>3716)
                     /locus_tag="PLY56_09770"
     CDS             complement(2831..>3716)
                     /locus_tag="PLY56_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HOJ21810.1"
                     /translation="GNVTGPILSIIDLLDDFQQANIALERLTDVYDTKPEEDLAKKAP
                     IHLPPLRGHIVFQNVTFRYPTRPDKNALQNINLEIHPGQTVALVGRSGAGKTTFASIL
                     LRMHAPSEGKVYLDGYDLEQVSLSSLRSQVGVVPQEVTLFSGTIRENIAFGQPNAPLD
                     EVVGAAMLAGAHDFITALPLGYETVIGERGQSLSGGQRQRIAIARALFKKPRILIFDE
                     ATSALDNESERAIQQNLDKILKDRTTFIIAHRLSTVRNADLIVVLDQGVIVETGTHYS
                     LMQQRGLYYYLNSQQLDQ"
     assembly_gap    3717..3726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3727..3969)
                     /locus_tag="PLY56_09775"
     CDS             complement(<3727..3969)
                     /locus_tag="PLY56_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ21811.1"
                     /translation="MVEAVNAINTIKAMAAERPTRWKWEGLMVRALNVEFRSAITTML
                     LSASGNVLQNLQRIFLLWFGAHLVIEGRLTVGQLMAF"
BASE COUNT          667 a         1328 c         1242 g          731 t
ORIGIN      
        1 gaagagcaac accaccgggc cgcgcaacag cgtatcggcg gtccaccgag ccgattgaac
       61 gatcacatag gagaggtgcc cgaagagggt cagcgggggc atgagaagcg ttccgatcag
      121 gagcgcccgc cagggaatgc cgtccagctc cttcgccggt agttcaaact cttgtctctt
      181 ctcttccgtt gccggcatct atcgcgttcc ttctctcttc tccccgcagt gcgcacggcc
      241 ccacaggcgg gactcttttc ccgctcgagg gctccgtttc ctatctgccg ggcccgcccc
      301 cggcgctccg gggaggcggc tccccacact cctgaagaac tcctagctgg ggccgcctga
      361 tggcttgcgc cagaccaggg agtagcgcca gaagaaatgc cggcggatga tcgcccctgg
      421 tagctccgcg gcgcacagcc ggcgcacttc cgcaagcgtc aggtacgtat cgtgccggcc
      481 atgagcatcc cacgccgcgc ggatctccgc gggcacccgc aggctctgcc ctcgaaccag
      541 gcgcatcgcg atgttgacag gcaccaccag cgcgctcatc agcctatccg cccgagttcc
      601 ctgaaacagg tccagcacca gaaggactcc acccggccgc aacgcccgcc ttgccttccg
      661 ggcgatctcc gcgaacggaa ggtggtgcca ggtggcgatg gaggcgatgc aatcgtaggc
      721 tccctcctcc agcggaaatt gcatcacgtc agctacgtgg tagtcgatgt tagcgcgctc
      781 ggccgagccc tctcgcgcca cgcggatcat ctccggggag aggtctaacg cgaggacacg
      841 gcgcgagcgc gcggccagga ggcgcgtgaa ctctccggtg ccgcagccga tgtcgagagc
      901 aagttcgcag cgcggcggcg cgtgcttcag caggtaacga tggtagtggt tgttgtggtc
      961 ccaacgttcc ctggttgaga gcctggcaat ccggtcgaaa tccgcgcgaa tctcgtccac
     1021 taccgcctcc tctcacgctc ttccccctca ccattccggg gaagccagtg cgcttcctct
     1081 tggcccgggc atggacaccc ggcttgcaaa ccaatcgcct gtagggcgca ggagccggcg
     1141 ctctccatgt ggaagtagct cataggccag catcctgagc gggcggatcc cggatcgtgc
     1201 cccttgcgcc cggcctatgg caagccatga tgaggaggga acgatggggt tcgcggtcga
     1261 attgcacttc gatccgcagg cagaaatgag agtacggagc ctctgggacg aactcgcccg
     1321 gcaggggatc cgctcactgc cgcctctgat cggctctcgg ccgcacatca gcatcgccgt
     1381 cttcgaccgc gtggatccag cgcgggttcg ccccgagctg gaggaactgg ccgccactct
     1441 tgctccccta ccgctcacct ttggtgctgt cggcaccttt cccgccaagg agggcgtcgt
     1501 cttcctcacc ccggtggtca ctcccgagct gctggagctc caccagtccg ttcatgcccg
     1561 gcttgatgca cttggcctgc gcgccctgga gcattaccgc ccggggcgat gggtgcccca
     1621 ctgcaccgtg gcgattgatc tgccccgcga gcagctactc ccggcgctgg agatctgccg
     1681 atccagccgc gtcttcggtt ccctgacgct cgctgagatc ggcctcgtgg agtatcgtcc
     1741 ggtgcgtcct atttgctcct tcccgctccg cgatccgcgg caggaaggct agacgcgggg
     1801 agacccgcgc ctgtcacttc gcgctgccca ggtcaccctg taccttcttg aacggggcca
     1861 ggaagaggct gagcacgctc ttgcggtcgg tcaccacttc gatggtggcg gtcataccag
     1921 ggcgcagcgg aaactcccgg gcgtaggctg tgatgaccgg ccggtcgggc gccacggtca
     1981 ccttgtacac ggagccgagc ttctcatcgc tctgcgcgtc gggcgagacg tcgaccacgg
     2041 tgccgttgac tacgccgtaa tcttggtacg gataggcatc gaacttcagc ttggcgggca
     2101 ggcccttgcg cacgaaggcg atctgctggt ttggcatctt cccctcgacc accaagcggg
     2161 catcggcagg cgccacggtc gcgacgggat cccccggctt gacaacggcg cccggggcac
     2221 gcacttcgag ggaggtgacc accccctcca ccggggaacg aatggtggag cgggcaagcg
     2281 ccagatcgag agcggcaatc tcgctttcga gccgggcgag cgcgctctcc ttctcaagcg
     2341 cctcgaccac ggaagcgccc gaggcgcgta gctgcaggag ttgctcttcc agcgtgcgca
     2401 tctcttgccg ggcattgtct cgccggaccc gtgcctcggc ggaatcgagc tggataagcg
     2461 cctcgttcgc ctcgacccgg tcgccctcgc ggacaaggat gaactgcacg acgccatccg
     2521 tctgggcttc gacgcggcgg gtgtagcctt ccggcagcag agtgccgcgt gcctcggcga
     2581 ctacgtcgag cacgctgaag tgcgcccaga ggccggcggc aagaaggaac gccacgatga
     2641 tgtagagcaa gccacgagcg gcccaccacg gcatctccgc caggatctcg gagacctccg
     2701 gcgtggtctc attcgcttgt gggggcgtcc acgtcttgcc cagggccgct agatcgagcg
     2761 gctcgcccac cgaggtttgc tcttccgtgc cagccattcc cttctccatc ctcaccttct
     2821 cggcactcgg ctactgatcc aactgctggc tgttcaggta gtagtaaagc ccgcgctgtt
     2881 gcatcaggct atagtgggta cccgtctcca cgatcacccc ttgatccagg accacgatca
     2941 aatcagcatt gcgcaccgtg ctcaggcggt gcgcgatgat gaaggtcgtg cgatccttga
     3001 ggatcttatc gaggttctgc tggatcgcgc gctcgctctc gttatcgagg gcgctcgttg
     3061 cctcgtcgaa aatcagaatg cgcggtttct tgaagagggc gcgcgcgatg gcgatgcgct
     3121 ggcgttgccc gccagagagg ctctgcccgc gctcaccgat caccgtctcg taaccgaggg
     3181 gaagcgcggt gatgaaatcg tgggcgccgg ccagcatcgc cgcgccgacc acttcatcca
     3241 aaggcgcgtt cggctgcccg aaagcgatgt tctcgcggat cgtgccgctg aagagcgtca
     3301 cctcctgggg caccacgccc acttgcgagc gcaggctgga gagcgaaacc tgctccaggt
     3361 cgtacccatc cagatagacc ttgccttcgc tcggcgcgtg catccgcagg agaatactgg
     3421 cgaaagtcgt ctttccggcg ccgctgcgcc cgacgagggc aacagtctgc ccgggatgga
     3481 tctccaggtt gatattctgg agcgcgttct tatcgggccg ggtcgggtat cgaaacgtca
     3541 cgttctggaa gacgatatgc ccgcgcaacg gcgggagatg gatcggcgcc ttcttggcca
     3601 gatcttcctc gggcttggtg tcgtaaacgt ctgtgaggcg ctccagagcg atattcgcct
     3661 gttggaagtc atccaggagg tcaatgatgc tgaggatcgg tcccgtgaca ttgccgnnnn
     3721 nnnnnngaag gccatcaact ggccaacggt gagccggccc tcgatcacca ggtgcgcgcc
     3781 gaaccaaagg aggaagatgc gctgcagatt ctgaagcacg ttgccggagg cgctgaggag
     3841 catggtggtg atggcgcttc gaaactccac gttgagcgcc cgcaccatca ggccttccca
     3901 cttccaccgc gtggggcgct ccgccgccat cgccttgatg gtgttgatcg cgttcaccgc
     3961 ctccaccatc tgcgattc
//
LOCUS       DAOSCV010000364         3977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14325_length_3977_cov_3.439317, whole genome shotgun sequence.
ACCESSION   DAOSCV010000364 DAOSCV010000000
VERSION     DAOSCV010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14325_length_3977_cov_3.439317"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..933)
                     /locus_tag="PLY56_09780"
     CDS             complement(67..933)
                     /locus_tag="PLY56_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21812.1"
                     /translation="MAEIVDERVASHRRQSRIIYALLAGLMVLFLLFELRLPFIVGPQ
                     SRGLFETVEKLPEDKLALLILNWAPGTYGENYPQTEALIQHFFLAGKPFAIFGVDPVG
                     PTLGHEIAERHAARLGKQYGVDWVTWGYRTLQLPMILGLKRDIPGTIQRDYQGRSLEQ
                     FPIMRGIRSARDLSLIVDVTPSATVEIWIQYFHGAVGTPVGYAPTAVMAPEAYPYLQS
                     KQLVGMLAGIKGAAEYAALLDEHYEEELTWKYPPMRAMNAISIAHVLIVALIILGNYQ
                     YFTRHRRRREQS"
     gene            complement(937..1752)
                     /locus_tag="PLY56_09785"
     CDS             complement(937..1752)
                     /locus_tag="PLY56_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21813.1"
                     /translation="MLTTDTFTLWRNIVLGFVAGIALLLLLDRITNNSSRRRLMVILT
                     FLGGLFYLLEFVLPPGTTLGFLPAPADPTRDNALSPFISTIGSITLVIAGFSFGLGLL
                     NLATIHGRNILGQRSGWHNSLAFFVAMILMVISGLFRGEYASLAALYRVLFEGLYQPL
                     GASVFSVLAFFITTAAYRAFRIRSGEAALMMGAAFIVMMAQVPMGMMLTNWLPETGWA
                     ANLRIEQVSNWLMTILNMAALRAVAFGIGIGGLAMSLRIWLSLERGTYFGKDS"
     gene            complement(1775..2608)
                     /locus_tag="PLY56_09790"
     CDS             complement(1775..2608)
                     /locus_tag="PLY56_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21814.1"
                     /translation="MKLQIEHASAAVLVLEGLLCVVILGKIFAARGGRAPFIRRIPGL
                     AALDESVGRATEMGRPMLFSLGLGGLDIVTLQALAVLGHVARLAARYATRVIVPVVDA
                     VIFPVTEEVLRDAYTAEGRPELFDSNDVRFLSDQQFAYASGVMGIIGREQVASSFLFG
                     SFFAESLILAEAGHQVGAMQVAGTPSTTQIPFFIAACDYTIIGEEYYAASAYLTREPV
                     FLGSLIGQDWGKIALLAIIILMVGAASVWSLTHGGVMDDFQSRVIAFWQWMPGAPAAG
                     Q"
     gene            complement(2610..2936)
                     /locus_tag="PLY56_09795"
     CDS             complement(2610..2936)
                     /locus_tag="PLY56_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21815.1"
                     /translation="MLRDELTAEQRDEITEKLAQRVVKRRLESVATLFLELNKPIAFI
                     GGQALLVGAPIFGTLFGYENMQRLALYFQSPENVERLLVRIEELSQERDAQEKGGAEP
                     SAEGSP"
     gene            complement(2923..3387)
                     /locus_tag="PLY56_09800"
     CDS             complement(2923..3387)
                     /locus_tag="PLY56_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21816.1"
                     /translation="MTGAHPAGAVLEWRVHLARERPRALLFVAGVLCASAALAYAVFG
                     HPLPVLITLALLFSSVSEFVLPVTYRIAPEGVTRRNGVNITTLAWNEVRRMEVYEEGV
                     RLSPFARPSALEPFRGILIRYPADAGMRQHVLDALRVYSPEAGPKGVSDAEG"
BASE COUNT          818 a         1302 c         1236 g          621 t
ORIGIN      
        1 tgtagagaat ggtgaagatc gccagcgtgg agatgcacgc cagcagtgtg atcacgcctt
       61 ccaggctcat gactgctctc tccgccgtcg gtgccgcgtg aagtactggt agttccccag
      121 gatgatgagc gcgacgatca agacgtgggc gatcgagatg gcgttcatcg cgcgcatcgg
      181 cggatacttc caggtgagct cctcctcata atgctcatcc aggagcgccg cgtactccgc
      241 cgcgcccttg atcccggcca gcatccccac gagctgcttc gattgcaggt agggatatgc
      301 ttccggggcc atgacggccg tgggcgcgta gcccaccgga gtgcccaccg cgccgtggaa
      361 gtactgaatc cagatctcca ccgtcgcgga cggggtaaca tctacgatca ggctgaggtc
      421 gcgcgccgac cgaatccccc gcatgatggg aaactgctcc agggaacgac cctggtagtc
      481 gcgctggata gtgccaggta tgtcgcgctt gaggcccagg atcatcggga gctgcagggt
      541 gcggtagccc caggtaaccc aatcgacgcc atactgcttg cccaggcgcg ccgcatggcg
      601 ctcggcaatc tcgtgaccca gggtgggccc gaccgggtcg accccaaaga tggcgaacgg
      661 cttaccggcc aggaagaaat gctggatcag cgcctccgtc tgtgggtagt tctctccata
      721 ggtgcccggc gcccagttga ggatcaggag ggcgagttta tcctccggca gcttctccac
      781 ggtctcgaac agcccgcgcg actgcgggcc cacgatgaag gggaggcgca gctcgaagag
      841 caggaagagc accatcagcc cggcgagaag ggcgtagatg atccggctct gccggcgatg
      901 ggaggcaaca cgctcgtcga ctatctcggc catgccctac gagtcctttc caaagtaggt
      961 gcctctctcc agactgagcc agatgcgaag cgacattgcc agccctccaa tgccgatccc
     1021 aaacgcgacg gcgcgcaaag ccgccatgtt gaggatggtc atcagccagt tgctcacctg
     1081 ctcgatgcgc agatttgcgg cccaccccgt ttctggcagc cagttggtca gcatcatccc
     1141 catcggcacc tgcgccatca tcacgatgaa tgccgcgccc atcatcagcg cggcttcgcc
     1201 agaacggatt cggaaggcac ggtaggccgc ggtggtgatg aagaaggcca gcacgctgaa
     1261 gacactcgcc ccgagcggct ggtaaaggcc ttcgaagagc acccgataaa gggcggccag
     1321 ggaggcgtat tcgcccctga aaagcccgga gatgaccatc aagatcatcg cgacaaagaa
     1381 cgcgagcgag ttgtgccacc ccgaccgctg tcccaggatg ttccgcccgt ggatcgtcgc
     1441 caggttgagc aggcccagcc cgaaggaaaa accagcgatc accagagtga tgctcccgat
     1501 ggtggagatg aacgggctga gcgcgttatc gcgcgtggga tccgcgggcg cgggcaggaa
     1561 gccaagcgtc gtgcccggcg ggagaacaaa ctccaggaga tagaagagcc cgccgaggaa
     1621 ggtgagaatc accatcaggc gccgccgcga gctgttgttc gtgatgcggt ctagcagcag
     1681 caagagggcg atccccgcca cgaacccgag gacaatattc cgccagagag taaacgtatc
     1741 cgtcgtcaac atcgtcgctt gctatccccg catttcactg gccggccgcg ggtgcgccgg
     1801 gcatccactg ccagaaggcg atcaccctgc tctggaagtc atccatcacg ccaccatgcg
     1861 tgagcgacca gaccgaggcc gcgccgacca tgaggatgat gatcgccagg agcgcgatct
     1921 tcccccaatc ctgcccgatc aggctgccca ggaagacggg ctcacgagtc aggtaggcgc
     1981 tcgcggcgta atactcctcc ccgatgatcg tatagtcgca agccgcaatg aagaagggaa
     2041 tctgcgtggt agagggcgtt ccggccacct gcatggcgcc gacctggtgg cccgcctcgg
     2101 caaggatcag cgattccgcg aaaaaggagc caaaaaggaa gctggacgcc acctgctcgc
     2161 ggccaataat ccccatcacg cccgaagcgt aggcgaactg ctggtccgag aggaagcgga
     2221 cgtcattcga atcgaacagc tcgggccggc cctcggccgt gtaggcatcg cggaggacct
     2281 cctctgtcac cgggaagatc accgcgtcta ccacgggcac gatgacgcgg gtggcgtagc
     2341 gcgcggcgag gcgcgccaca tggcccagca ccgcgagcgc ctggagcgtg acgatatcca
     2401 ggccgcccag accgagagag aagagcatcg ggcgccccat ttcggtggcg cggcccacgc
     2461 tctcgtccag ggcggccagc ccgggaatcc gccggatgaa gggggcgcgc ccgccccgcg
     2521 cggcgaagat ctttcccagg atcaccacgc acagcagccc ctcgagtacc aggactgcag
     2581 cgctcgcatg ctcgatctgt agcttcacgc tacggactcc cttccgcaga cggctccgcg
     2641 ccgcctttct cttgggcatc gcgctcctgg ctgagctcct cgatacgcac gagcagccgc
     2701 tcgacattct cgggcgactg gaaatagagc gccaggcgct gcatattctc atagccaaag
     2761 agcgtgccga agatgggcgc tcccaccagc aacgcctgcc ccccaatgaa ggcgatcggc
     2821 ttgttcaact ccaggaaaag cgtagctacg ctctccagac gccgtttcac cacgcgctgt
     2881 gccagcttct ccgtgatctc atcgcgctgt tccgcggtca gctcatccct cagcatcgga
     2941 tactcctttc ggacccgctt cgggcgaata tacccggagc gcatcgagca cgtgctgccg
     3001 cattcccgcg tccgcgggat agcggatcag gatgccccgg aacggctcca gcgcggatgg
     3061 cctggcgaac ggactcaacc ggactccctc ttcgtagacc tccatccgcc ggacctcatt
     3121 ccaggcgagg gtggtgatgt tcacgccatt gcgccgcgtg acgccctccg gcgcaatgcg
     3181 gtaggtgacc ggcagcacga attccgaaac cgacgaaaag agcagggcca gcgtgatgag
     3241 aacaggcaaa gggtggccga agacggcgta ggccagcgcg gcactggcac acagcacgcc
     3301 ggccacgaag agtaaggcgc gcgggcgctc acgcgccaga tgaacgcgcc actccaagac
     3361 agccccggcg gggtgagccc ccgtcacgga ccgctccggc ttggtctgag acggcaactc
     3421 cgcccctgaa cgccgttcgt aagaatccaa agtaatggcc cgccaatatg ctaagcgaca
     3481 gagcgcacca ccgcgacgcg ctccccagat aggacagaac ctacccggaa gcaggttccg
     3541 agtaggctct ccttcgaatc ggaatgccgc atcactgcga cattccgcca tcccacccac
     3601 gattgccgga gcgcagaaac gatcgcgctt ttccgcaccc cattccgaat gcgcacgcag
     3661 accgctgcct ccgggcgatg gtcatagata attgtgaacg tgaagtatac tagcataggc
     3721 gcgagtctgg tgtcaacgcc ggatccggcc agcttgggat tccgagataa gggttccgca
     3781 gtctcgaaga cggcgcgcaa cccggcgcac gagggctaac ctccgcgctg cttgtgagcg
     3841 aagccctgcc gaactggatg gggcgacact cccgggcacc ctagagcggc acgggctagg
     3901 aggggaagcc cgaagggctt tgccgggaag cagcgcggag cttcgagctc cgcgcgtgcg
     3961 ggcggccctc tccttat
//
LOCUS       DAOSCV010000365         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14370_length_3967_cov_3.732362, whole genome shotgun sequence.
ACCESSION   DAOSCV010000365 DAOSCV010000000
VERSION     DAOSCV010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_3967_cov_3.732362"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLY56_09805"
     CDS             complement(<1..502)
                     /locus_tag="PLY56_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ21817.1"
                     /translation="MRRKLIPILILAGVAVGVPLALRARVGSDQKPEFQTATVERGDI
                     TVTVSATGTLQPLTTVDIKSRAGGRVDVLAVDIGSIVKPGQLIAKIDPTDTLANVRTA
                     QADSAASRAKVEQANTNLRLQKLQSEAQIRQAEEALRSARARLAQAEKQAKVQPNLTK
                     SSIAQAQ"
     gene            complement(606..1868)
                     /locus_tag="PLY56_09810"
     CDS             complement(606..1868)
                     /locus_tag="PLY56_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ21818.1"
                     /translation="MPALLSICVWGALACRPSAAAEAESQAWTLESCIRTALEQHGDI
                     LSARENVNAAEAQKRSAASSLFYPKLSLQSTHTEGYTETDRQGTDRSSQQNLLRLGVT
                     FWDGGVQRQNVRRARAGEESAQAALDRTRQGLVYQVTSAYLELLRAQHAQAVAERKLS
                     QAEAQKVMVEARIKAGAAAEVEIYPIEVEIANARVEKIRAANDVRVAGSALRNAMGIG
                     PGGVPEITDLPAEIADAPALEACLEEAAGRRPEIRQAKAALEQQQAGLDLARLNLRPL
                     INVGGGLDRGFAGTYNPNQWSLSATLSWSLYDRADRSQVDAARATLHAAEEQLQQVTR
                     DIAAEVEQAHLALTSARERIAAAEASVAMARKNLEVAEARYKQGLAIPIEIVDAQVAF
                     SNAELQAISARYDYLLARTQLDRAIGSL"
     gene            complement(2593..3195)
                     /locus_tag="PLY56_09815"
     CDS             complement(2593..3195)
                     /locus_tag="PLY56_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOJ21819.1"
                     /translation="MKKTHTALLMGASMLLATSLAWAEGPAGGERPHRGPKMHCAPTL
                     NLTDAQKTKIKAIREKYRGQRQELHKKMEAEVSSVLTREQREQVAKMREARAKRMAWR
                     RTAPRPGGPRANARRLSQELNLTPKQEQKLQEIRDARRAEMRSLFQDARSGKVTRDQA
                     RERAQALREKTTRQMNDVLTRDQQQKLKSLREQWGACCRK"
     gene            complement(3350..3814)
                     /locus_tag="PLY56_09820"
     CDS             complement(3350..3814)
                     /locus_tag="PLY56_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOJ21820.1"
                     /translation="MSVSTDGSRRKAFRAFVSFVVLLAFIVLAVTGLVMYAWRAIGSR
                     EFLGLTRGAWNAVHTSISFLSILAAAAHVYLNWRVLVSYVKRRTSAARSYLRECVVAL
                     GLVVAVIVGTLLGIPPFNHIAPTERPRRPPIEAPMGAPAATPDVPAGPDGPR"
BASE COUNT          605 a         1310 c         1305 g          747 t
ORIGIN      
        1 cctgtgcctg ggcgatgctg ctcttcgtca ggtttggctg gactttggcc tgcttctcgg
       61 cctgcgcgag ccgcgcgcgc gctgatctca gcgcctcctc ggcctggcga atctgtgcct
      121 cgctctgcag cttctgcagg cgcaggttgg tgttcgcctg ctccaccttt gcccgcgagg
      181 cagccgagtc tgcctgggcc gtgcgcacgt tcgcgagcgt gtccgtcggg tcgatcttgg
      241 cgatcaactg ccccggcttc actatgctgc cgatatccac tgccagcacg tccacccgac
      301 cgccagcgcg cgacttgata tccactgtgg tcagtggctg gagcgtgccg gtggcgctga
      361 ccgtgaccgt gatgtcaccg cgctccaccg tggcggtttg gaactcgggc ttctggtccg
      421 agccaacccg ggcgcgcagc gccaggggga cgccgacggc gactccggca aggatcagga
      481 ttgggatgag tttccttcgc atctcttctc ctcgacgtgg gagctatgca gagcatagag
      541 ctcgaatcgt ggatatggca tcgggcgctc gctcgctgct ctgtccccaa atcccgcgcg
      601 cgtcgctaga gggatccgat ggcccggtca agctgcgtcc gggccagcag gtagtcgtag
      661 cgcgcgctga tcgcctgcag ctccgcgttg ctgaaggcga cttgggcatc aacaatctcg
      721 atcgggatgg ccagaccctg cttgtagcgg gcctcggcca cttccaggtt tttgcgcgcc
      781 atcgccacgc tcgcctcggc ggcggcgatc cgctcgcgcg cgctggtcag cgccaggtgc
      841 gcctgctcca cctcggcggc gatatcgcgc gtcacctgct gcagctgctc ctcagccgcg
      901 tgcagggtgg cacgggcggc atccacctgg gatcggtcgg cgcggtcgta gaggctccac
      961 gagagcgtcg ccgacaggct ccactggttg gggttgtagg tgccagcgaa gccgcggtcg
     1021 aggcctcctc cgacgttgat cagcggtctc aggttgaggc gcgcgagatc aagtccggcc
     1081 tgctgctgct cgagggccgc cttcgcctgg cgaatctcgg gtcggcgccc ggccgcctct
     1141 tcaaggcaag cctccagggc gggagcatcg gcaatctctg ccgggagatc ggtgatctcg
     1201 ggcacgccgc cgggccctat ccccatggcg ttgcgcagcg cggatccggc gacgcgcacg
     1261 tcattggcgg cccggatctt ctctacgcgt gcattggcga tctccacctc gatcgggtag
     1321 atctccacct ccgcggcagc ccctgctttg attcgcgcct cgaccatcac cttctgcgct
     1381 tccgcctgcg atagcttccg ctcggcgacc gcctgcgcgt gctgcgcgcg caacaactcg
     1441 agataggcgc tcgtcacctg gtagaccagg ccctggcggg tgcgatccag ggccgcctgc
     1501 gcgctctcct cacccgcgcg cgcccggcgc acgttctggc gctgcacgcc gccatcccag
     1561 aaagtgacgc ccagccggag gaggttctgc tggctcgatc tatctgttcc ctgacgatcc
     1621 gtctcggtgt atccctcggt gtgagtggat tgcagcgaga gcttggggta gaaaagcgac
     1681 gatgcggcgc tgcgcttctg ggcctcggcc gcattcacgt tctcgcgtgc ggagagaatg
     1741 tcgccgtgct gctccagtgc ggtgcggatg cacgattcca gcgtccacgc ctggctctcc
     1801 gcttccgcag cggctgacgg gcggcatgcc agcgcccccc agacgcagat tgaaaggagc
     1861 gccggcaccc aacaccagcg tgcatgcctc tgccagccgg cgacgggcgt tgccccgcga
     1921 caccttccgc cataggagac gggtgtttct cctcctgccg gaggcgccca gcatcctgca
     1981 atatgaccca cgaggccggt gtcatggccc cgcaggggtg gtcggttcct gttcggcatc
     2041 gtttccttcc cttatctcct catcggtcat ggccctcatg gccgtcgtgg gtatcggttg
     2101 cctcccccag gaaggcttgc ttgaatctta acaggcaaag atgaagcaat tatgaaggag
     2161 agaggaaagc ggcaagaaga agagtgggcg atgagcgccg tttcgcgcgt aaagggaacg
     2221 atcgcgtggg gctcgcggac gacgccgaag gcgtccaaca tcccggctca gggtctcctg
     2281 accctggggc tcgcggccca aaagcacctg ggccccttaa aggggccgaa aacacgtgaa
     2341 gggggggtgt ccgacctctt gaggccgtgg gtattctata cggcgcatga cccagggtgc
     2401 agacaaccct gggctgtgtt gtgcgcccct ttcatgggcg agaatgtgct ccccccacgg
     2461 gcatcgatac cacggatgaa ggggactgac acggacgggc agggcgggcg tcggggggtg
     2521 gaagggctgc tacgggccgg ggaaggcggc cgggggcggg ttaccggtcc cccggccgct
     2581 gtcgtaggga gatcacttcc ggcagcatgc cccccactgc tcgcggagcg acttcagctt
     2641 ctgctgctgg tcgcgggtga gaacgtcgtt catctggcga gtggtcttct cgcgaagcgc
     2701 ctgggcgcgc tcgcgcgcct ggtcgcgggt taccttgccg gagcgagcgt cctggaacag
     2761 cgagcgcatc tctgcgcggc gagcatcgcg gatctcctgc agcttctgct cctgcttcgg
     2821 tgtcaggttc agctcctgcg agagacgccg ggcattggcg cgagggccac ccggtcgggg
     2881 cgcggtacgg cgccaggcca tcctcttggc gcgggcttcg cgcatcttcg cgacttgctc
     2941 ccgctgctcg cgcgtgagga cggacgagac ctctgcctcc atcttcttgt gcagctcctg
     3001 gcgctgcccg cggtacttct cgcgaatcgc cttgatcttc gtcttctgcg cgtcggtcag
     3061 gttgagcgtg ggcgcgcagt gcatcttcgg gcctcggtgc ggacgttccc ctcccgccgg
     3121 accctcagcc caggcaaggc tcgtggcgag aagcatgctg gcacccatca gcagggcggt
     3181 gtgagtcttc ttcatcgtct cttatcttct ccctccgttg tgttctatca cacgactggt
     3241 tggcgggccc gccccgttgg atatgggcga cgcccggctc tctacgatga gtatagcaaa
     3301 caaatatgaa ggaatgatga aacgccgcgt ctcgccgcgg cgggcgttcc taacgagggc
     3361 catccgggcc ggcgggcacg tctggcgtgg ccgccggcgc ccccataggc gcctcgatag
     3421 gaggccgtct tgggcgctcc gttggcgcga tgtggttgaa cggtgggatg cccagaagtg
     3481 ttcccacgat cacggctacc acgaggccga gggccaccac gcactcgcgc aggtaggatc
     3541 gcgccgcgct cgtgcgccgt ttcacgtagg agacgagcac tctccagttg agatagacgt
     3601 gcgctgcggc ggccaggatc gacagaaagc tgatgctggt atgcaccgcg ttccatgctc
     3661 cccgggtcaa acccaggaac tcgcgcgagc cgattgcgcg ccaggcgtac atcacgagac
     3721 ccgtgactgc cagcacgatg aacgcgagca gtacaacaaa agaaacgaat gcccgaaagg
     3781 ctttcctgcg gcttccatcc gtcgaaacag acaccggttg aacctcctct ccgcggtcca
     3841 gatggcctgc atcaggccac gcttccacgc ttctatgtta ctgcggaatc gtgaaggaag
     3901 catgaagccc cgctgggatt ccgacataag gagaaggccg cgcgcacgcg cggagctcga
     3961 agctccg
//
LOCUS       DAOSCV010000366         3952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14439_length_3952_cov_3.151142, whole genome shotgun sequence.
ACCESSION   DAOSCV010000366 DAOSCV010000000
VERSION     DAOSCV010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14439_length_3952_cov_3.151142"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1298
                     /gene="dnaN"
                     /locus_tag="PLY56_09825"
     CDS             171..1298
                     /gene="dnaN"
                     /locus_tag="PLY56_09825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ21821.1"
                     /translation="MKIVCRQENLAKGLSIVGRAVATRSALPVLSNILLATDESRLKL
                     SATNLEISVNCWVGADVKEEGAITVPARLLTNFVNSLPPEPIELNLIARTQTLNLRCG
                     RYEANIKGIDAQEFPLILVPEEDKAASFATADLAKMIDCVVFAAATDESRPVLTGVYT
                     ELTPQGVTLAAADSYRLSVISSTIQAPPERVSIIIPARALQELRRISGEAESVTMLVS
                     STRNQVFFRMPEVELISQLVKGTFPDYRQIIPTTHNTRVVATTAELLPALRMASIFAR
                     DAANIVRLQAIPNDGGVGRLIVSATSAEHGDNVSELDVTVDGQPIEIAFNVRYLLDAL
                     EVIDTAQVALETRDPTSPGVIKPVGGPDFVHIIMPMHIFGA"
     gene            complement(1425..1757)
                     /locus_tag="PLY56_09830"
     CDS             complement(1425..1757)
                     /locus_tag="PLY56_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21822.1"
                     /translation="MDVRDALKLLALRAPSLQGEALKALSAPPAAATLRYRTILQLAL
                     CDPEANWTDEERLALLDVLAPPSQGEERTEQLNIRLSPSEVAIVRMMAEAEGMTVSAY
                     VRKRILSE"
     gene            complement(1812..2150)
                     /locus_tag="PLY56_09835"
     CDS             complement(1812..2150)
                     /locus_tag="PLY56_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21823.1"
                     /translation="MERQLWILHDDRIGGNDPVPFWATREEGLADITAQCREIGYEVP
                     ADLALDDVGVYLAPADDRGRPRWDTPAHLLRAAHGIACAVATWEAADTRWDALGALLG
                     ALGEALLGWE"
     gene            complement(2219..2689)
                     /locus_tag="PLY56_09840"
     CDS             complement(2219..2689)
                     /locus_tag="PLY56_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21824.1"
                     /translation="MTDRINLRVRKPGGGHYTTILEGGTFGEMLAAVKALPGRRYIQC
                     YRAWEVTLEQAQALEARFAAGRERQRQEAERQRQQREAERQRAAEEAERRRLTAPATP
                     RQIAYLKDLMAGLGHSNWQPGRLTMAEASTLIHQLLESGDAIDIAPHNPMSEVY"
     gene            2889..3104
                     /locus_tag="PLY56_09845"
     CDS             2889..3104
                     /locus_tag="PLY56_09845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HOJ21825.1"
                     /translation="MSDTSPEWLSIQREAVLSILDSLTPRQREAVLLRLDGLSQREIA
                     QKMGISPRAVRRLVQRARRNVPLFALK"
     gene            3227..3373
                     /locus_tag="PLY56_09850"
     CDS             3227..3373
                     /locus_tag="PLY56_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21826.1"
                     /translation="MGTWPAWAQQLYRDAQRERRSERRWQHFVVRSRDEDELEEIDEG
                     RLIL"
     gene            3373..3660
                     /locus_tag="PLY56_09855"
     CDS             3373..3660
                     /locus_tag="PLY56_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOJ21827.1"
                     /translation="MTTEQRDVWRRVIERDGGRCLLCGRSGSEVHHIISRRYRSAWDV
                     RNMCVLCRACHSRAHGKRMRGALLAEMVRRYGYDYSDPPWAGLLAAYGEGI"
     gene            3657..>3952
                     /locus_tag="PLY56_09860"
     CDS             3657..>3952
                     /locus_tag="PLY56_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21828.1"
                     /translation="MTDSPSIWRNRIVGYGEQPASQFLANPNNWRIHPQAQREALRGA
                     LNEVGWVQAVIVNRRTGYLVDGHERVWQALQNGDALVPYIEVDLSPEEEAYVLAT"
BASE COUNT          748 a         1211 c         1197 g          796 t
ORIGIN      
        1 agcatgggcg tatgcagggc cattgccttg cgatacggcg ggaatgttga ggaggaggaa
       61 tgatgcttga ggagaagcca tgagcgagcc aatggccgtc gcgagatgta tgaaagttat
      121 ctgcgctccc tctatgaggg ggatggcgcg atacgaaggg aggataaaaa atgaaaattg
      181 tctgtcgtca agagaatctt gccaaagggc tttccatcgt agggcgagcg gtagccacgc
      241 ggagcgccct gcccgtgctc tcgaacattc ttctggccac cgacgaatcg cgcctgaaac
      301 tttcggccac caacctagag atcagcgtga actgctgggt gggcgccgat gtcaaggaag
      361 agggagccat caccgtgccg gcccgcctgc tcaccaattt cgtgaattcc ctgccgccgg
      421 aacccatcga actcaacctc atcgcgcgca cgcagacgct caacctccgc tgtggccgct
      481 atgaggcgaa catcaaaggg attgacgccc aagaattccc gctcatcctc gtgcccgagg
      541 aagataaggc cgcttctttt gctacggcgg acctcgccaa gatgattgac tgcgttgtgt
      601 ttgccgccgc tacagacgaa agccgccccg tgctgacggg cgtctatacc gaactcacgc
      661 cccaaggagt tacccttgcc gccgccgaca gttatcgcct ttccgtaatc tcttccacca
      721 tccaagcgcc ccccgaacgt gtgagcatta tcatcccggc gcgcgccttg caagagttgc
      781 gccgcatcag cggcgaagcg gaatccgtca caatgctcgt ttcttccacg cgcaaccagg
      841 tctttttccg catgccggag gtggaattga tctcgcaact cgtcaagggc accttcccag
      901 attaccgtca gatcatcccg accacccaca atacgcgtgt ggtagccact accgccgaac
      961 ttttgccagc cctgcgtatg gcctccatct ttgcgcgcga tgcggcgaat atcgtgcgcc
     1021 tacaggccat ccccaacgat ggtggcgtgg gccggctgat cgtctccgcc acttcggcag
     1081 agcacggtga taacgtcagc gaactggatg taaccgtcga tggccaaccc atcgaaatcg
     1141 ctttcaatgt gcgctatttg ttagatgccc ttgaggtgat tgatacagcg caggtggcct
     1201 tagagacgcg cgaccccacc agcccagggg tcatcaagcc ggtgggcggg ccagatttcg
     1261 tgcacatcat tatgccgatg catatctttg gcgcataggc gctactagag gcggatgagc
     1321 gggcctttca cgccctctat ggggattact gcctcatcgt gtgggatgtg gagggggtag
     1381 aaaagatcag cgcccaggat gtgtatcatc ctgggcgctg acggttattc tgacagtatc
     1441 cgtttacgca cataagcgga tactgtcatc ccctccgcct cggccatcat ccgcacgatg
     1501 gccacttcgg agggagacag gcggatgttg agttgctccg ttcgctcttc cccttgcgag
     1561 ggaggagcaa ggacatcgag gagcgcgagg cgctcctcat ccgtccagtt ggcctcaggg
     1621 tcgcagaggg ccaactgaag gatggttctg tagcgcaggg tggcagcagc cggcggtgcg
     1681 ctgagggcct tcagggcctc gccctgcagg ctcggcgcgc gcaaggcaag aagtttcaat
     1741 gcgtcacgga cgtccatcct tcctcctctt tgccggggcc tcaccaccga ccccggcggg
     1801 ggctgctgtg gttactccca gcccagtagg gcttccccta gggcgcccag cagggcgccc
     1861 agggcatccc accgggtgtc cgccgcctcc cacgtggcca ccgcgcacgc gataccgtgc
     1921 gcggcgcgca ggaggtgggc gggcgtgtcc caccgcgggc gcccacggtc atccgcgggc
     1981 gccaggtaca cgcctacgtc gtccagggcg aggtccgccg ggacctcgta gccgatctca
     2041 cggcactggg ccgtgatgtc ggccaggccc tcctcccgcg tggcccaaaa tggcacgggg
     2101 tcgttaccac cgatccggtc gtcgtgcaga atccaaagtt gccgctccat cgcaaacctc
     2161 ccgaaaaata ttttttgccg gggcctgacc ccctgccccg gcgtggggct gtcagggttt
     2221 agtacacctc ggacatcggg ttgtgcggtg cgatgtcgat cgcatcgccg ctctccagca
     2281 gttgatgaat caacgtgcta gcctcggcca tcgttagtct gcccggctgc cagttactgt
     2341 gaccgagacc ggccatcagg tccttcaggt acgcgatctg ccgcggtgtc gccggggccg
     2401 tcagccgccg ccgctcggcc tcctcggccg cccgttgccg ctccgcctcg cgctgttgcc
     2461 gttgccgttc ggcctcctgc cgctgccgct cgcggccagc cgcgaagcgg gcttccagtg
     2521 cctgggcctg ctcgagggtg acctcccagg ccctatagca ttggatatac cgtcggcccg
     2581 ggagggcctt gacggctgcg agcatctcac cgaacgtccc gccctccagg atggtggtgt
     2641 agtgaccacc ccctggcttc ctaacccgaa ggttaatccg atctgtcata gttccctcct
     2701 ctcattttct acacatagta tagcatatac tatacgtttt gtcaaggggg tttggggcca
     2761 attttcaggg aaaattttgg aatttacgga agttgcaaag gccggctcaa cttgttgact
     2821 tgcagtttat aaaatgatac tattcctgta ggaattacag gagttatccc atagcgggtt
     2881 agcggcatat gtccgatact tctccagaat ggctgtccat ccagcgcgag gccgtccttt
     2941 ctatcctcga cagcctcact ccccgccaac gtgaggccgt cctcctccgc ctcgatgggc
     3001 tatcccagcg cgaaatcgcc cagaaaatgg gcataagccc ccgtgcagtg cgccgtcttg
     3061 tgcaaagagc gcgccgaaat gttcctcttt ttgctctcaa atgactatag atagcagagg
     3121 ggaagtaatg tctaccgctt cgcgctgcga gatttgcgag cggcccgtca cgaacgacca
     3181 ttttttctgt gccgaatgcc ggcggatata cggcctcacg ggaagaatgg gcacgtggcc
     3241 ggcctgggca cagcaactct accgagacgc ccagcgtgag cggcgctccg aacgccgttg
     3301 gcagcacttc gtggtgcgca gcagagacga ggatgagttg gaagagatag acgaggggcg
     3361 gttgatcctg tgatgaccac cgagcagcgc gatgtgtgga ggcgggtgat cgaacgggat
     3421 ggcgggcgtt gcctcctgtg tggccggtcc ggttcggagg tgcaccacat catcagccgc
     3481 cgctatcgca gcgcatggga tgtgcgcaat atgtgcgtcc tctgccgcgc atgccacagc
     3541 cgggcgcacg gcaagcggat gcgaggtgcc ctactcgcgg agatggtgcg gcggtatggt
     3601 tacgattaca gcgatccgcc gtgggccggg ttactcgcgg cttatggcga gggaatatga
     3661 cggatagccc atccatttgg cgcaatcgga tcgtaggata tggcgagcag ccggcctcgc
     3721 agttcctggc caatcccaac aactggcgca ttcacccaca ggcacaacgc gaggccctgc
     3781 gcggggcact caatgaggta ggctgggtgc aggcggtgat cgtcaatcgc cgcacgggat
     3841 atttggtgga tggccacgag cgggtttggc aggctctgca gaatggcgat gcattggtgc
     3901 cctatatcga ggtggattta tcacccgagg aagaggccta cgtcctggcc ac
//
LOCUS       DAOSCV010000367         3949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14458_length_3949_cov_3.824088, whole genome shotgun sequence.
ACCESSION   DAOSCV010000367 DAOSCV010000000
VERSION     DAOSCV010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14458_length_3949_cov_3.824088"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1327)
                     /locus_tag="PLY56_09865"
     CDS             complement(170..1327)
                     /locus_tag="PLY56_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ21829.1"
                     /translation="MEELRSERHPARAEIRKRVLALLLVFAAGVACGAVGYHVPVHRI
                     AGISPADGQQNGTQTPPVRPTTPGGLCGAESNVIQVARAVGPAVVTIVNMQRPSGSSE
                     LRRAGFGSGFIVRSDGLILTNHHVVAGADRVDVVTLGDDPIPARVLGADPRIDIAVLQ
                     VERRNLPVVPIGSSDRLQVGQQAIAIGNSLGFERTVTVGVLSALDRAIPGAGTPLRDL
                     IQTDAAIHPGNSGGPLLDSCGRAIGVNTAAVRGPAGTGGIGFAVPINTAMRAVDDVTR
                     HGRIIVPWMGISYAEISENLARAYDLPVEEGVIVSAVAANSPAARAGLRSGDIIVSLD
                     GAPLTSAGRLQELIREADVGQRVQLGILRDGRRQTLTLVLEEMPREQTVGG"
     gene            1646..>3949
                     /locus_tag="PLY56_09870"
     CDS             1646..>3949
                     /locus_tag="PLY56_09870"
                     /inference="COORDINATES: protein motif:HMM:NF037334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21830.1"
                     /translation="MRHLLLLAALVLLVIPVAGQQEMVFEAEDVSSPKAAWGENITPE
                     NLWNLWSKDTNADKKWSGGRVLQSPRVMADRETPEEGAPVLHTVLTGIPKGKWLVTIK
                     FGRGLAVSLDGKEWKRLSDLGGRLGQFEIRDGRFEFWVDDRYADPNNPGFTYYDCITL
                     TPCPPEVNGVMNGDFEFGSDIRSSGWSFWSREGVTTVDLAREGTKGTRCAKIVNTGER
                     DWYLANSGRTKVKPRQTWIATARMKCVDTASATLHVFGVSQGKVTYSALATDGVWGTT
                     DWTEVRAVAEIPADVDEIYLRVNGYGKAQVWVDDVALRPGPPVPAPKPKQKVNGWAKE
                     RVWEKLDRGLVAMPTEDGKVYLGWRLLESDPETVAFNVYRASGNAAPVRLNQAPITRT
                     TDFVDENPALGSESVYTVRPVVGGKEGPVGGEARLPANPEVKPYLSIKLQGDYTFQKV
                     GVADLNGDGRLDYVIKTPQDNIDPAHSYWTPSPDTYKLEAYLHDGTFLWRYDMGWAIE
                     RGIWYSPYVVWDLDGDGKAEVAVKAGEGDPRGPDGRVHRGPEYCVILDGLTGKPRARV
                     EWPKREAFGGGLTGYNYASRNQMGIAYLDGKTPCLLLARGTYSVMQLVAYQFRRGRLQ
                     ELWRWDNREETVGNWRGQGAHWMHSGDVDGDGRDEVVLGSCTIDDDGKGLWTTGLGHP
                     DRCFLTDIDPSRPGLEIFYHIEPRQKENGLCIVDAATGAILWGLKEQTWHVGSGMCAD
                     IDPRYPGMECWAAEDPKGDPTGQRYHGN"
BASE COUNT          692 a         1307 c         1323 g          627 t
ORIGIN      
        1 tgacacgcgt tcgtctggga ggcgaccggg agccgccggg cctgctatgg cgcgccgatt
       61 tgcgacgcca ccgatccgtg atgaggccgc gaggtcctca gaaccgcggg gagccgggcg
      121 ccccgcgaca aacgcccggc gggctccact ctcctgcgca gtgatcgggc tatccgccga
      181 cagtctgctc gcggggcatc tcctccaaca ccagggtgag cgtctggcgg cggccatcac
      241 gcaagatgcc gagctgaacg cgctggccca catcggcctc gcggatcagc tcctgaagcc
      301 ggcccgcgct cgtgagcggc gcgccatcca gcgatacgat gatatcgcct gaacggagcc
      361 cggcgcgtgc tgccgggctg ttggctgcca cggcgctcac gatcacgcct tcttcgaccg
      421 gcaggtcgta ggcacgtgcg aggttctcgg agatctccgc gtaggagatt cccatccacg
      481 gcacgatgat tcgcccgtgg cgggtgacgt cgtccacggc gcgcatcgcc gtgttgatcg
      541 gcaccgcgaa gccgatgccc cctgtgccgg ccgggccccg aactgccgcc gtgttcacgc
      601 cgatggcgcg cccgcagcta tcgaggagcg gcccgccact gttcccgggg tggatcgccg
      661 cgtctgtctg gatcagatcg cgcagaggcg tccccgcgcc tggaatcgcc cgatccagtg
      721 cgctcaacac gcccacggtc accgtgcgct cgaaaccgag agagtttccg atcgcgatcg
      781 cctgctgccc cacctggagc cggtccgaac tgccaatcgg aacgactggc aggttccgcc
      841 tttccacctg gagcacggcg atgtcgatgc gcgggtccgc ccccaacacc cgcgcgggaa
      901 ttggatcgtc gcctagcgtg accacatcaa cgcgatcggc tcccgcgacg acatggtggt
      961 tcgtcaggat caagccgtcg cttctgacga tgaagcccga tccaaagcca gcgcgccgca
     1021 gctcgctact cccactcggg cgctgcatat tcactatcgt gaccaccgcg gggcccaccg
     1081 cacgtgccac ctggatgacg ttgctctcgg cgccacacaa cccgccgggg gtcgttggcc
     1141 ggaccggggg cgtttgcgtg ccgttctgct gtccatctgc cggggagatc cccgcgatgc
     1201 ggtgaacagg cacgtgatac cccaccgcgc cgcaggccac tcccgccgca aagacgagca
     1261 agagcgcgag aacgcgcttc cttatctcgg cgcgggccgg gtgtcgctcg cttctcagct
     1321 cttccatatc ggctcctctt cactccctcc aggccggcac ctccgcgttg gcgggccgga
     1381 ggaagctgtg tcgccctccc cattgcctgg cgtataccca tattcacggg ccagttcgac
     1441 gcgctcggga gcgaggttgg ccgacctccc ggttcatcgg cccgcgcagg gagagggatg
     1501 ggcgctccgc cacggcctga acctgctttc aggcgccacc tcaccccggg caggactcgc
     1561 catgccggtg cacaataaga gggcaaagaa taccagggca caagaggccc atcgctaatc
     1621 gggctcgccc atctggaggt aggatatgcg acatctgctc ttactcgccg cgctggtcct
     1681 ccttgtgatt cccgtcgcgg gccagcagga gatggtattt gaagcggagg atgtcagctc
     1741 gcccaaagcg gcctggggcg agaacatcac gccagaaaac ctctggaacc tatggagcaa
     1801 ggatacaaac gcggacaaga agtggtccgg cggcagagtc ctccagtctc ccagggtgat
     1861 ggccgaccgg gagacgcccg aggagggggc cccggtgctg cacacggtgc tcaccggcat
     1921 cccgaagggc aagtggctcg tcacgatcaa gttcgggagg ggactggccg tctcgctaga
     1981 tggcaaagag tggaagcgcc tctccgatct gggcgggcgc ctgggccaat ttgagatccg
     2041 ggacgggcgc ttcgagttct gggtggatga ccgctacgct gacccgaaca accccgggtt
     2101 cacctactac gattgcatca cgctcacgcc ctgcccgccc gaggtgaacg gcgtcatgaa
     2161 cggcgacttc gagttcggta gcgatatccg ctccagcggg tggtcgttct ggtcgcgcga
     2221 aggtgtcacc acggtcgatc tggcgcggga gggcaccaaa ggcacgcgct gcgcgaagat
     2281 cgtgaacacc ggcgagcgcg actggtacct cgccaactcc gggcggacca aagtgaaacc
     2341 gcgccagacg tggatcgcca ccgcccgcat gaagtgcgtc gacaccgcct ctgccacgct
     2401 ccatgtcttc ggcgtctctc aagggaaggt gacctactcg gcgctcgcca cggatggcgt
     2461 gtgggggacg accgactgga cggaggtacg cgccgtggcc gaaattccgg ccgatgtcga
     2521 cgagatctac ctgcgggtga acgggtacgg caaggcccag gtctgggtcg atgacgtggc
     2581 tctccgcccg ggcccgccgg tgccggcacc gaagccaaag caaaaggtaa acgggtgggc
     2641 gaaagagcgt gtttgggaaa agctggatcg cggcctcgtc gccatgccta ccgaggatgg
     2701 caaggtctac ctgggctggc gcctgctgga gagcgacccg gagacggtgg cgttcaacgt
     2761 ctatcgcgcc tcggggaacg cggctccggt gcgcctgaat caggcgccga tcacccggac
     2821 caccgacttc gtcgatgaga acccggccct gggtagcgag agcgtctaca ccgtgcgccc
     2881 ggtggtcggt ggcaaggagg gtccggttgg cggcgaagcg cgcctgccgg ccaatccgga
     2941 ggtgaagccc tatctctcca tcaagctcca gggtgactac accttccaga aggtaggcgt
     3001 ggccgacctg aacggcgatg gccggctcga ctacgtgatc aagacgccgc aggataacat
     3061 cgacccggcg cactcctact ggacgcccag cccggacacc tacaagctgg aggcctacct
     3121 gcacgacggc accttcctgt ggcgctacga catgggctgg gccatcgagc gcggcatctg
     3181 gtactcgccc tatgtcgtat gggacctgga tggcgatggc aaagcggaag tggccgtgaa
     3241 ggcgggagag ggtgacccgc gcgggcccga tggccgcgtc caccggggtc cggagtactg
     3301 tgtcatcctc gacggcctta ctggcaagcc gcgagcgcgc gtcgagtggc ccaagcgcga
     3361 ggctttcggc ggcggtctga ccggatacaa ctatgccagc cgcaaccaga tggggatcgc
     3421 ctacctggat ggaaagacgc cctgcctgct gctcgcccgc gggacgtact ccgtgatgca
     3481 gctggtcgcc tatcagttcc gtcggggccg gctgcaagag ctgtggcgtt gggacaaccg
     3541 cgaggaaact gtggggaact ggcgcggcca gggcgcgcac tggatgcaca gcggcgatgt
     3601 ggatggcgac ggccgggatg aagtggtcct tggctcgtgt accatcgatg acgacggcaa
     3661 gggcctctgg acgaccggcc tgggtcaccc agaccgctgc ttcctgacgg atattgatcc
     3721 ctcgcgcccc ggcctggaga tcttctacca cattgagccg cgccagaagg agaacgggct
     3781 ttgcatcgtg gacgcggcaa cgggcgcgat tctctggggc ctgaaggagc agacgtggca
     3841 cgtcggctcg ggcatgtgcg ccgatatcga cccgcgctat ccgggcatgg agtgctgggc
     3901 cgcggaagac cccaagggcg acccgacggg ccagcgctac cacggcaac
//
LOCUS       DAOSCV010000368         3945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14478_length_3945_cov_4.124679, whole genome shotgun sequence.
ACCESSION   DAOSCV010000368 DAOSCV010000000
VERSION     DAOSCV010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_3945_cov_4.124679"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..331
                     /locus_tag="PLY56_09875"
     CDS             176..331
                     /locus_tag="PLY56_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21831.1"
                     /translation="MPEELMGFGLMPGAGDTSSGIAGLFFKAHNARVAFAGKQRSPLG
                     PRLLDHA"
     gene            348..776
                     /locus_tag="PLY56_09880"
     CDS             348..776
                     /locus_tag="PLY56_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21832.1"
                     /translation="MRRKRRWRALYTLLLLVGGSGALAHFFLAARPVQAGDIRRDPQA
                     FAGRHVTVTGTVLGSASLGGALMSAFGGGAGFFLLDDGSGQIGVTVTGLPPAKGKRVR
                     VRGRVEILASLSLPDSWAKVFGGAGELSAVVVRAEELSVE"
     gene            1104..2225
                     /locus_tag="PLY56_09885"
     CDS             1104..2225
                     /locus_tag="PLY56_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21833.1"
                     /translation="MIMLSAFSGSSDRQPDNRRVALLKSLTTNNRFYLLVDPDSGLKN
                     ADTIERFPEESVLGIAYPCLEDGLNDFAIIRSTFETLDQFPDDEELRTELMEIIADAQ
                     RKLVLRLEELRDRISDGQAHFLGTDLEEFKGHIVNLATKGVPRKTLDSMMKLDIFETV
                     ASTEALLKGDFAPEFVYIYEPLPENPLDELLQMPPRPFIGRIFDDVLKSVLAEENYGD
                     RHIFDRNVFQKFVALMFVNYATTDPVFYGTNKADYGVSADKDMDQTPLYVAVGKALRL
                     LERQRQQELQGQGESPQEAPSAAADAPELSPTDQLVLARLLPICDTARILVTLDNGRA
                     RECGEYILREVHHLFTDLQEIGVMSSGAPSPVEQDYRDE"
     gene            2377..3396
                     /locus_tag="PLY56_09890"
     CDS             2377..3396
                     /locus_tag="PLY56_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HOJ21834.1"
                     /translation="MLRQLRLRARRRGATLSPPHSTRRVSTSLWVLCLLLLLAAGLPG
                     VAQPGSGDTPEAWLILREPGLELLYQTGHESVARRAARAATAAIWRISSELGSEPDQP
                     LMLVIHPTHQAFARAVGSRRGQMIVGIASGERAVAHIDGSGALADLDEVIAHEVAHLL
                     LAQAVGGARVPRWFDEGYAEHACGSLEWASRELLADHLEMGRLASLDELSDSFPRDGQ
                     AAAVAYAQSHALVAYLLREAPAGALPELARRLRAGEKFENAVIAATGMSVSAWDEAWR
                     RDFHGPSRWYPWIAFLAGASGIAMSVLCLLAYRAIRRRIEALDEGDEYDRPISIRRAR
                     RWRRR"
     gene            3347..>3945
                     /locus_tag="PLY56_09895"
     CDS             3347..>3945
                     /locus_tag="PLY56_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOJ21835.1"
                     /translation="MTDLSRYGVRGDGVADDTAALQRALDETAGELRLAPGTYRLTRP
                     LEVRLDRGRRAISGSGGTARLLMAGLGPAVRLTGTHRGSAAPEQTAGEVADSQRMPVL
                     FDLEIAGEHPEADGVEIAYAWQPTLSRVQVQRCRHGIHVVANNRNVLISDCHIYHNAG
                     AGIFLDKVGLHQINIIGSHISYNRRGGIKVWRSEIRNLQ"
BASE COUNT          633 a         1227 c         1389 g          696 t
ORIGIN      
        1 aatgcccccc ggcggtgaca cgtggggaga atggctgtat gggcttcgcc tgccattgcc
       61 cggtccgccc gacaccccgc aggtggtccc cctgcccggc atggaggggg ttctcgcgcg
      121 cgtgagtggc gataaaccgg cggatagacc gggatccaca gatgaacaca ggtaaatgcc
      181 agaagagctg atgggctttg gcttgatgcc gggtgcgggc gatacttcct ccggaatagc
      241 cggtttgttt ttcaaagctc ataacgcccg cgtggccttt gctggaaagc agcgcagtcc
      301 tttaggtccg cgcctgttgg accacgccta acggttggga ggtggagatg cggcgtaaga
      361 gacggtggcg tgctctttat accctgctcc tcctggtggg ggggagcggt gcattggccc
      421 acttcttcct cgcggcccgg ccggtgcagg caggcgatat ccggcgagac ccgcaggcgt
      481 ttgctgggcg gcatgtgacg gtgaccggta ctgttctggg gagcgcatcg ctgggagggg
      541 cgctgatgag cgccttcggg ggcggcgccg gctttttcct cctcgacgac ggctccgggc
      601 agatcggcgt cacggttacc ggtctcccgc cggccaaggg gaagcgagtg cgcgtgcggg
      661 ggagggtcga gatcctggcc tcgctcagcc ttccggattc gtgggccaag gtgtttggcg
      721 gcgcggggga gctgtcggcg gtggtggtga gagcggagga gctgagcgtc gagtagcaag
      781 ccgggcgaat tgcgtggaag ccctgcaagg agaacgggcc agggtggcga aaccagattc
      841 taggtgatgt aggagcacct cttcgcgtga agaggtgcga gcgcgttttg gcctggccgg
      901 aaggccaggg ccatgctgat agagtgtgga gacccgccag ggggactggc gaggtatctt
      961 gggggaatgc ctgtgagtgg gcggtctgca tccggtgcgg gctgcaggat tcccatggca
     1021 gcccgccgct cgagggccgg ctgggcatct tggcctggcc gccgcgcgcg gccgacagag
     1081 tggcattcgc gaggaggggt tgaatgatca tgctgtccgc gtttagcggc agttcagacc
     1141 ggcagccgga caaccgcagg gtggctttgc tcaagagtct gaccactaat aatcgcttct
     1201 atctcctggt cgaccccgat agcggcctga aaaacgccga tactatagaa cgctttcccg
     1261 aggaatcggt gcttgggatt gcctaccctt gcctcgagga cggactcaac gacttcgcga
     1321 tcatccgctc gaccttcgaa acgctcgacc agtttcccga tgatgaggag ctgcgcaccg
     1381 agctgatgga gatcatcgct gacgcccagc gtaaactcgt cctgcgcctc gaggagttgc
     1441 gcgaccggat cagcgatggc caggcccatt ttctgggcac cgacctggag gagttcaagg
     1501 gccatatcgt caacctggcg accaagggag tgcctcgcaa gaccctggac agcatgatga
     1561 agctggacat cttcgagacg gttgccagca ccgaggccct cctcaagggc gactttgctc
     1621 cagagttcgt ctacatctac gagcccctgc cagagaatcc cctggatgag cttctgcaga
     1681 tgccgccgcg cccgtttatc gggcggatct tcgacgatgt cctcaagagc gtgctcgccg
     1741 aagagaacta tggcgaccgc catatcttcg accggaacgt cttccagaag ttcgtcgccc
     1801 tgatgttcgt gaactacgct acgacggacc cggtcttcta cggcaccaac aaggcggatt
     1861 acggcgtatc tgccgataag gatatggacc aaacgcccct ctatgtggcg gttggcaaag
     1921 ccttgcgcct gctggagcgg cagcgccagc aagagttgca ggggcaggga gagagccccc
     1981 aggaggctcc cagcgcggct gcggatgcgc ccgagctttc tcccacggac cagctggttc
     2041 tggcccggct gctccccatc tgcgacaccg cccggatact ggtgacgctc gataatggcc
     2101 gggcgcgtga gtgtggcgag tacattttgc gcgaggtgca tcacctcttc accgatctgc
     2161 aggagatagg cgtgatgtcg tctggcgcgc ctagccccgt ggagcaagat tatcgcgacg
     2221 agtgaccgag agcccagagc cgcgggcgtc ggccgcgcgg ctttgggcgg gatggttttc
     2281 gccgtgcact ctcggagagg gcggcgccgt ggtagctcca ccgcgtctcc cgttgcagca
     2341 ggatggccac gaaggagcag cgcaaggccg gtcggcgtgc tgcgccagct gcggctccgg
     2401 gctcgccgga gaggagcgac actctcgccg ccgcattcca cgcgccgtgt cagcacctcg
     2461 ctgtgggttc tctgcctcct ccttctcctt gccgcaggcc tgcccggcgt tgcgcagccc
     2521 ggatccggcg ataccccgga agcgtggctg atcctccgcg agccaggtct cgaactcctc
     2581 taccagactg gtcacgagag cgtggcccgg cgcgcggccc gcgcggccac ggccgctatc
     2641 tggcggatct cttccgagtt ggggagcgag ccggaccagc cgctcatgct cgtcattcac
     2701 ccgacgcacc aggcgttcgc gcgggccgta ggctcgcgcc gcggacagat gatcgtgggg
     2761 atcgcgtctg gcgagcgggc ggtggcgcac atcgatggga gcggcgcgct cgccgatctc
     2821 gacgaggtga tcgcgcacga ggtggcccac ctgctcctcg cgcaggccgt gggcggcgcg
     2881 cgcgtccccc ggtggtttga cgagggctac gcggagcacg cctgcggcag tctcgagtgg
     2941 gcctcgcggg agctactcgc ggatcacctg gagatgggcc gcctggcttc cctggacgag
     3001 ctttcggata gcttcccgcg cgatgggcag gctgccgccg tggcgtatgc ccagagccac
     3061 gccctggtgg cttaccttct gcgcgaggcg cccgccggcg cgctcccgga gctggcccgg
     3121 cgcctgcgcg cgggcgagaa gttcgagaac gcggtgatcg ccgcgaccgg gatgtccgtt
     3181 tccgcctggg acgaggcgtg gcggcgcgat tttcacgggc cctcgcgctg gtacccgtgg
     3241 atcgcctttc tggccggcgc cagcgggatc gcgatgtcgg tgctctgcct tctggcctac
     3301 cgggcgatcc gtcggcgcat cgaggccctg gatgagggag atgagtatga ccgacctatc
     3361 tcgatacggc gtgcgcggcg atggcgtcgc cgatgacact gcggcgctgc agcgcgccct
     3421 tgatgagacc gcgggcgagc tgcgcctggc gcccggcacc taccggctga cgcgccctct
     3481 ggaggtgcgc ctcgaccggg gacgcagagc gatcagcggc tcggggggca ccgcgcgcct
     3541 gctgatggct gggctgggcc cggcggtacg gctcaccggc acgcaccgcg gaagcgccgc
     3601 gcccgagcag actgccgggg aagttgctga tagtcagcgg atgccggtgc ttttcgacct
     3661 ggagatcgcc ggcgagcatc ccgaggcgga tggcgtggag atcgcctatg cctggcagcc
     3721 gacgctgagc cgcgtgcagg tgcagcgctg ccggcatggg attcatgtgg tcgcgaacaa
     3781 ccggaacgtt ctgatcagcg attgccacat ctaccacaac gccggcgccg ggatcttcct
     3841 cgacaaggtg gggctgcacc agatcaacat catcggctcg cacatcagct acaaccggcg
     3901 cggtggcatc aaggtgtggc ggagcgagat ccgcaacctg cagat
//
LOCUS       DAOSCV010000369         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14520_length_3937_cov_3.609480, whole genome shotgun sequence.
ACCESSION   DAOSCV010000369 DAOSCV010000000
VERSION     DAOSCV010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_3937_cov_3.609480"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3937)
                     /locus_tag="PLY56_09900"
     CDS             complement(<1..>3937)
                     /locus_tag="PLY56_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21836.1"
                     /translation="ADESSSEEPKEIALGQSRIPVVTVCDAEQYDCSLRVLAGEDARI
                     YWAWQDARNHYRLDITRSQMALYRVRGGHVEKLASASGAFLEARKPMPLTLRRRANAL
                     YVIARGQIALDCLDSTFSGGPVAGKNLEAAGFQPAEEVFFSDDFMRTEKEQELGDWTK
                     VSGTWQFHSVKETNVHADFRLSVNPFSLGGKDPEGAVVTVGFPFWCDYTASVSVKSQG
                     GVAGLVFGYQGEEDYFLLRMDLRAPYPRPSGVELLRITPQGPRVLASGTVRAASGDWY
                     RLGVRTDGPRVQAILDGDVLFDLIQPGCIGGPVGLYAQGEAECFFDDVKVEPTGVCAF
                     DRAYSLRRNGRPLGEGWRFIAAPGERIQGDTGPHHLVHEGTELARYVLGDPGWQGGVI
                     QTRVQVEGPGGWAGVLFGYQDERNWRLARWRVADGSAAQGGGTAAAGARLQYIRCIDG
                     KKQIAAESTAALENGTWHDLLVDCTNEGYTHIYLDGRLRLRVADPVPAQGRAGLYSFG
                     ATRARFADTAFRLARDVQVEQTISNEVFRNDPFMKLWSSSYGQWFPVKQVPNVYWNKG
                     DFFNSYQIAMPITPGLVLAFATEGEDFGQGYRVSIEPVKGEKETCRVHLSRLEQIVKT
                     AKIQDVSKAPKLTLHRSGDVVWAMLGSRELFSFHDPRPLPGRRVGIATPAKFDLANVE
                     VRRENVADYPFEEAGCDWEQYGNWVVTNRFSCTPTWSHLSALADRACAMWNKYAFSGD
                     LTVEFYAGMRQTNKALSYPRIGDMNVTICGDGRRLDSGYSYVVGAWDPGWTEKWTQLL
                     RGTEVVEQTDRYLAPRVRESSGGRQIEVPWTAEGRPIHGAWYYIKIRRVGNRVECYFD
                     NVKVLTYEDGKPLDGRFLAIWTQMNEVVFARASITYEHRYAPKRLLTRGTPLTPLPEP
                     GGPKGPLPVLASSTHPGFVECFESPETVWKPTGPARDVVVERVAGGPAGSRGCLRVTN
                     IAPGGDLGAAVPLPSDIDASRAVVEFDYRIPKDAKIHLYVTLNGQRHFVRLTGTEPDN
                     AILPLLGEIPIRADGQWHRARFDLGYAVSRLYPGKRPLRIGEMLFGHFHEGYLRAGIG
                     GNGPGVSYAIDNFIVATASATTPVLTWSPPQVPADAKSATQAANPETDKPTFLFSVDR
                     DPLAEPQPTKPTTETKATLKDLGEGLWFAHVRWLQPDGAWSRTAHLPIHVVVQPLSLQ
                     AAELREDAPWGGDPIRFRVQPATGPHLDLSRLAVTVNGRKIDRPARIAEYDPVKRQLS
                     LDLQRASLTLADQQKVKLEVQAATADGPVNKTALSVVVSASADTRPPDPP"
BASE COUNT          676 a         1308 c         1295 g          658 t
ORIGIN      
        1 cgggggatcc ggcgggcgcg tatcggcaga ggcgctgacg acgacgctca gtgccgtctt
       61 gttcaccggt ccgtcagcag tagccgcctg cacctctagc ttcaccttct gctggtcggc
      121 cagagtgagg ctggcgcgtt ggagatcgag cgagagctgc cgcttcactg gatcgtactc
      181 ggcgatgcgc gccgggcgat cgattttgcg accgttgaca gtcactgcca ggcgagagag
      241 gtcaaggtgc gggccggtgg caggctgcac gcggaagcga atcggatcgc cgccccaggg
      301 agcatcttct cgaagctcag ccgcttgcag tgaaagcggc tgaacgacca cgtggattgg
      361 caggtgagcg gtgcggctcc aggcgccatc cggctgcagc cagcggacat gcgcgaacca
      421 caggccctcg cccagatcct tcagcgtggc cttcgtctct gtcgtcggct tcgttggctg
      481 gggctctgcc agcgggtcgc gatcgaccga gaagaggaag gtaggcttat ctgtctctgg
      541 gttggccgct tgagtggccg acttcgcatc cgcgggcacc tgcggaggcg accaggtgag
      601 cacgggcgtt gtcgcggagg ccgtcgccac gatgaagtta tcgatggcgt aggagacgcc
      661 cggcccattg ccgccaatac cggcgcgcag atagccctca tggaaatgac cgaagagcat
      721 ctcgccaatg cggagtggcc gcttgcccgg atagagccgc gagacggcat agcccaggtc
      781 gaaccgggcg cgatgccact ggccatcggc ccggatggga atctcgccca gcagcggcag
      841 aatcgcgttg tccggctcgg tgcccgtgag gcgcacgaag tggcgctggc cgttcagcgt
      901 gacgtagaga tggatcttcg cgtccttggg gatgcggtag tcgaactcca ccacggcgcg
      961 cgaagcgtcg atgtcgctcg ggagaggcac cgcggcaccc agatcgccgc cgggggcgat
     1021 gttggtcacg cgcaggcagc cccggctgcc cgccggaccg ccggcaacgc gctccaccac
     1081 gacatcgcgc gcaggcccag tcggcttcca cacggtttcc gggctctcga agcactccac
     1141 gaacccgggg tgcgtgctgg acgccagaac cgggaggggg cccttcggtc ctccgggttc
     1201 aggaagaggc gtgagcggag tgccccgcgt cagaagccgc ttcggcgcgt agcggtgctc
     1261 gtaggtgatg ctcgcacggg caaagacgac ctcgttcatc tgcgtccaga tcgccaggaa
     1321 gcgcccgtct agcggcttgc cgtcctcata ggtgagcact ttcacgttgt cgaagtagca
     1381 ctccacccgg ttgccaacgc ggcggatctt gatgtagtac cacgcgccat ggatcgggcg
     1441 gccttcggcg gtccagggca cctcaatctg gcggccaccg gatgactcgc gcacccgcgg
     1501 ggcgagatag cggtcggttt gctcaactac ctccgtgcca cggagaagct gcgtccattt
     1561 ctctgtccat cccgggtccc acgcgcccac cacgtaggag tagccgctat ctaggcgccg
     1621 gccatcgccg cagatcgtca cattcatgtc gccaatccgc gggtaggaga gcgccttgtt
     1681 ggtctggcgc atcccggcat agaactccac ggtgagatcg ccgctgaaag cgtacttgtt
     1741 ccacatggcg caggcgcggt cggcgagggc gctcagatgc gaccacgtcg gcgtgcagga
     1801 gaagcggttg gtcacaaccc agttgccata ctgctcccag tcgcagcccg cctcctcgaa
     1861 ggggtagtcg gccacgttct cgcggcgcac ctccacgttg gccagatcga acttggccgg
     1921 cgtcgcgatc cctacgcgcc gccccggcag cggcctggga tcgtggaagc tgaagagctc
     1981 tcgtgagcca agcatggccc agacgacatc accgctccgg tgcaatgtca gcttcggtgc
     2041 cttgctcaca tcctggatct tcgccgtctt gacaatctgc tccagccggc tcaggtgaac
     2101 ccggcaagtt tccttctccc ccttcaccgg ctcgatgctg acgcggtatc cctggccaaa
     2161 atcctcaccc tcggtggcga acgcgaggac caggccgggc gtgatcggca tcgcgatctg
     2221 gtagctgttg aagaagtcgc ccttgttcca gtatacgttc gggacctgct tcactggaaa
     2281 ccactggcca tacgaggacg accacagctt catgaagggg tcgttgcgga acacctcgtt
     2341 ggagatcgtc tgttccacct gcacatcgcg cgcgaggcga aacgccgtat cggcaaagcg
     2401 cgcgcgcgtg gccccgaagg agtagaggcc ggcccgtccc tgagcgggca ccggatcggc
     2461 gacacgcaac cggagccgcc catcgagata gatgtgcgta tagccctcgt tggtgcaatc
     2521 caccagaagg tcgtgccagg tgccgttctc caacgcggcg gtagactcgg cggcgatttg
     2581 cttcttcccg tcgatgcacc ggatgtactg gagccgcgct ccggcggccg ccgtgccacc
     2641 cccttgcgcg gcagatccat ccgccacgcg ccagcgggcc aggcgccagt tgcgctcgtc
     2701 ctgatagccg aaaagaacgc ccgcccaacc accgggccct tccacctgca cccgggtctg
     2761 gatgacaccc ccctgccacc cgggatcgcc aagaacgtag cgcgccagct ccgtgccctc
     2821 atgcacaagg tggtgcgggc cggtatcgcc ctggatgcgc tcgccgggcg ccgcaatgaa
     2881 acgccagccc tcgcccagcg gccggccatt ccggcgcaag gagtaggcgc gatcaaacgc
     2941 acagacgccc gtcggctcca ccttcacatc atcgaagaag cactcggctt cgccctgcgc
     3001 gtagaggcct accggaccgc caatgcaccc cggctggatc aagtcgaaca gcacgtcgcc
     3061 gtcgaggatg gcttgaacgc gcgggccgtc ggtccggacg ccgaggcggt accagtcgcc
     3121 cgaggcggcg cggacagtgc cggaggcgag gacgcgcgga ccctggggcg tgatgcggag
     3181 cagttcaact ccagaggggc gcgggtaggg cgcgcgcaaa tccatacgca ggaggaagta
     3241 gtcttcctcc ccctgatagc cgaagacgag gccggccacg cccccctggc tcttgacgga
     3301 gacgctggcg gtatagtcgc accagaaggg gaacccgacg gtgaccaccg cgccctcggg
     3361 gtcttttccg cccagcgaga aggggttgac gctgagccgg aagtcggcat ggacgtttgt
     3421 ctccttgacg gagtggaact gccaggtgcc gctcaccttc gtccaatcgc cgagctcctg
     3481 ctccttctcg gtgcgcatga aatcatcgct gaagaagacc tcttcggccg gctggaagcc
     3541 agcggcctcc aggttctttc ctgcgacggg acctcccgag aaggtggagt cgaggcagtc
     3601 gagcgcgatc tgcccgcgag cgatcacgta gagggcgttc gcgcgccggc gcagtgtgag
     3661 cggcatcggc ttccgggcct caaggaaggc cccggaggcg ctggcgagct tttcgacatg
     3721 cccgccacgc acccggtaga gcgccatctg cgagcgagtg atatcgaggc ggtagtggtt
     3781 tctggcatcc tgccatgccc agtaaatccg cgcgtcctcg cccgcgagca cccggaggga
     3841 gcagtcgtac tgctccgcgt cacacaccgt gacgacggga atgcggcttt gccctagtgc
     3901 gatctctttc ggctcctccg atgagctctc gtcggcg
//
LOCUS       DAOSCV010000370         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14539_length_3935_cov_2.991237, whole genome shotgun sequence.
ACCESSION   DAOSCV010000370 DAOSCV010000000
VERSION     DAOSCV010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14539_length_3935_cov_2.991237"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PLY56_09905"
     CDS             <1..1510
                     /locus_tag="PLY56_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ21837.1"
                     /translation="STEVAQARAAYQEARAELSNAERQLATTRTLARSGVFTQKSVDE
                     ICEKRDEIASELAARRVEHETELRDAEAAVRTAQARLDRAESARRLAEQELSRRKALV
                     AAGAVQYRPLEEARREMAEAERDCQQAETALRLADANLARAKKLFDTGVRSRRELEEA
                     QAARDAADAELKRAQEQLRIAKQVLARERELLDSGVYASREIQQAENELLQAEKARAE
                     AEAALHQAKQRLSIATSPEKKRVLVELENRLAALDSLLRRETSVARQDLYAQRETQAA
                     EAAVAQARARLLAARNTLKVLRAPVDGEGASVAVTAPIGGRVLERAANRGQVVTAQDV
                     LFTILDLSTVWVEARVYAKDARQVRAGQPVTIVASAFPDEKFTGKVSYMGDVIDEQTR
                     TLVVRCEVANPRRKLKPGVFVKVSIGLGRRQGALSVPASAVQREGERTFVFVATGRSE
                     FQEREVRLGTESGGYYEVLDGLKPGETVVTEGAFLVKSHGKKEELGHAGHAH"
     gene            1550..>3935
                     /locus_tag="PLY56_09910"
     CDS             1550..>3935
                     /locus_tag="PLY56_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOJ21838.1"
                     /translation="MINRLITFALEQRVLVLILAALLIGVGVKSALELPIDAVPDVTN
                     VQVQINTDAPGLSPFEVEKLITFPLELAMSGLPEMAELRSLSKFGLSQITVVFKDHVD
                     IYFARQLVSERLQDASQHLPPGVGAHPEMAPVSTGLGEIYQFVVERIPGANGVGAHSS
                     MSAIHRAEGIHRAEDEEHHRYLAPCGCPCPVCTRRDAGLVQSSPWLTPFDPDAMYLRT
                     LTDWVIKPQLRTVPGVAEVNPFGGFEKQYQVLVDPDRLVSYGLTLRDVFEALGRNNRN
                     AGGAYIEHGAEQYLVRGVGLVRNIEEIKNIVITAHDGIPIRVRDVAEVVVGPEVRQGS
                     ATKDGQGEVVTAVVMLLRGANSRTVVADVKKKVEALNKSLAPAGVRIVPFYDRTELVK
                     KTIRTVEKNLFEGAVLVIAVLFALLGNIRAALIVASAIPLSMLFALTGMQKYGISGNL
                     MSLGAIDFGLIVDGSVIMVENCVRRLTERRRELKRALTPDERLATVRAAALEVRQATQ
                     FGEMIIIASYLPILTLTGYEGKMFKPMALTVVFALTGAMILSLTLIPVLCALFLRDRP
                     VERENRVLGWVRRRYVPALRWAITHRRTTVLEATALFLAGVLLFPFLGSEFVPKLDEG
                     AIAFSAFKLPSVSLSEATRLATTIEKVLMEKFPDEVETAVTKTGRAEIATDAAGPDHG
                     DHFIILKPRSQWKRAKTKEELVNLMNAELSKVPGISYLFSQPIELRVNELISGVRSDV
                     AVKVFGEEIDILREKAEEIAAVLREVSGAADTKVEQVTGLPMLQIEVDRDAISRY"
BASE COUNT          690 a         1240 c         1410 g          595 t
ORIGIN      
        1 cagcaccgag gtggcgcaag cgcgagccgc gtaccaggaa gcgcgggcgg aactctccaa
       61 cgcggagcgc cagctggcga ccacacgcac actggcgcgc tcgggagtct tcacgcagaa
      121 atcggtggat gagatctgcg agaagcggga tgagatcgca agcgagctgg ctgcccggcg
      181 cgtggagcac gagaccgaac tccgggatgc cgaggctgcc gtgcggacgg cgcaggcgag
      241 gctcgaccgc gccgagagcg cgcgccgcct tgccgaacag gagttgagcc ggcgcaaggc
      301 gctcgtggca gccggcgcgg tgcagtaccg gcctctggaa gaggcgcgcc gcgagatggc
      361 ggaggcggag agggactgtc agcaggcgga gacggcgctg cgcctcgccg acgcgaacct
      421 ggcacgcgcg aagaagctgt ttgataccgg cgtccgctcc cgccgggagc tggaggaagc
      481 tcaggcggcg cgggatgcgg cggacgcgga gctgaagaga gctcaggagc agctgcggat
      541 cgcgaagcag gttctggcgc gagagcgcga gctgctcgat tccggcgtct atgccagccg
      601 cgagatccaa caggccgaga acgagctcct ccaggcggag aaggcccgcg ccgaggccga
      661 ggccgcgctc catcaggcga agcagcgcct cagcatcgcg acctcccccg agaagaaacg
      721 ggtgctggtc gagctggaga accggctggc ggcgctggat tcgctgctcc gccgggagac
      781 gagcgtagcg aggcaagacc tctacgcgca gcgcgagacg caggcagcgg aggcggcggt
      841 tgcccaggcc cgggcacgcc tgctagccgc ccgcaacacg ctcaaagtcc tgcgggcccc
      901 ggtggatggg gagggtgcga gcgtggcggt caccgcgccc attggcgggc gcgtcctgga
      961 gcgtgccgcg aaccgcggcc aagtcgtgac cgcgcaggac gtgctcttca cgatcctcga
     1021 cctctccacg gtgtgggtag aagcccgggt gtacgccaag gacgcgcgcc aggtgcgtgc
     1081 cggccaaccg gtcaccatcg tggcgagcgc cttcccagat gagaagttca ccggcaaggt
     1141 ctcctatatg ggagatgtga tcgacgagca aacgcgcacc ctggtggtgc ggtgcgaggt
     1201 cgccaaccca cggcgcaagc tgaagccggg cgtcttcgtc aaggtgagca tcggccttgg
     1261 ccggcggcag ggtgcgctct ccgtgcctgc cagcgcggtt cagcgcgaag gtgagaggac
     1321 gttcgtcttc gtcgccaccg gccggagcga gttccaggag cgagaagtcc gcctgggcac
     1381 ggagagcggc ggctattacg aggtgctcga cggcctgaag ccaggagaga cagtggtcac
     1441 tgaaggcgcg ttcctggtga agtctcacgg aaagaaggag gagctcggtc acgccgggca
     1501 cgcgcactga ccccactctg ccgcaaggga agagggaggc tctcgttcca tgatcaaccg
     1561 cctgatcact ttcgcgctgg agcagcgcgt gctcgtcctg atcctcgccg cgctgctcat
     1621 cggcgttggg gtgaagtcgg cgctcgaact gccgattgac gccgtgccgg acgtgaccaa
     1681 cgtccaggtg cagatcaaca ccgacgcgcc ggggctgtcg ccgttcgagg tggagaaact
     1741 gatcaccttc ccgctggagc tggcgatgag tggcctgccg gagatggccg agctccgcag
     1801 cctctccaag ttcgggctgt cgcagattac cgtcgtcttc aaggaccatg tggacatcta
     1861 cttcgcccgg cagttggtgt cggagcggct gcaggatgct tcgcagcacc tcccgcccgg
     1921 agtgggagcg cacccggaaa tggccccggt gagcaccgga ctgggggaga tctaccagtt
     1981 cgtggtggag cgcatcccag gggcgaacgg agtgggtgcc cactcctcca tgagcgctat
     2041 ccaccgcgcg gaggggatcc atagggcgga ggatgaggag caccaccgct acctggcgcc
     2101 ctgcggctgc ccctgcccgg tctgcacgcg cagggacgcg gggctcgtgc agagctcgcc
     2161 gtggctcacg ccgttcgatc ccgacgccat gtacctgcga acgctcaccg attgggtgat
     2221 caagccgcag cttcgcacgg tgccgggcgt ggccgaggtg aaccccttcg gcgggtttga
     2281 gaagcagtat caggtgttgg tcgacccaga ccggctggtg agctacgggc tgaccctgcg
     2341 agacgtgttc gaggcgctgg ggcgcaacaa ccgcaacgcc ggcggggcct acatcgagca
     2401 cggcgcggag cagtacctgg tgcgcggcgt gggcctggtc cgcaatatcg aagagatcaa
     2461 gaacatcgtg atcacggcgc acgacggcat ccccatccgg gtgcgcgacg tggccgaggt
     2521 ggtcgtcggc ccggaagtgc gccaggggag cgcgacgaag gatggccagg gagaagtcgt
     2581 caccgccgtg gtgatgctcc tgcgcggcgc gaacagccgg accgtcgtcg ccgatgtgaa
     2641 gaagaaggtc gaggctctca acaagagcct ggccccggcc ggcgtccgga ttgtcccctt
     2701 ctacgaccgc accgagctgg tgaagaagac gatccggacc gtcgagaaga acctcttcga
     2761 gggcgcggtg ctggtgatcg ccgtcctgtt cgcgctgttg gggaacatcc gggcggcgct
     2821 gatcgtggct tccgcgatcc cgctctcgat gctcttcgcg ctgacgggga tgcagaagta
     2881 cggcatctcc gggaatctga tgagtctcgg tgcaatcgac ttcgggctga tcgtggacgg
     2941 ctcggtgatc atggtggaga actgcgtgcg ccggctgaca gagcggcggc gcgagctaaa
     3001 gcgcgcgctg accccggacg aacggctggc gaccgtgcgc gccgccgcgt tggaggtgcg
     3061 ccaggcgacg cagttcggcg agatgatcat catcgcttcc tacctgccga tcctcacgct
     3121 caccgggtat gaaggcaaga tgttcaagcc gatggcgctc accgtcgtct tcgcgctcac
     3181 cggcgcgatg atcctcagcc tcacgctgat cccggtgctg tgcgccctct tcctgcgcga
     3241 ccggccggtg gagcgcgaga atcgcgtcct gggatgggtg cgccgtcgct acgtgcctgc
     3301 gctgcgctgg gcgataacac atcgccggac caccgtgctg gaggccaccg cactcttcct
     3361 ggccggcgtc ttgctcttcc cgttcctcgg ctcggagttc gtcccgaagc tggatgaggg
     3421 ggcaatcgcc ttctcggcct tcaagctgcc cagcgtgtcg ctctccgagg ccacgcgcct
     3481 ggccactacc atcgagaagg tgctgatgga gaagttcccg gatgaggtgg agacggcggt
     3541 cacgaagacc gggcgcgccg agatcgccac cgatgccgcc ggtccggacc acggcgacca
     3601 cttcatcata ctgaagccac gcagccagtg gaagcgcgcc aagaccaagg aggaactcgt
     3661 caacctgatg aacgccgagc tctccaaggt gcctggcatt tcctatctct tctcgcagcc
     3721 gatcgaactg cgcgtcaacg agctaatctc gggcgtgcgc tccgacgtgg cggtgaaggt
     3781 gttcggcgaa gagatcgaca tcctgcgcga gaaggccgag gagatcgcgg cggtgttgcg
     3841 cgaggtgtcc ggcgcggcgg acacgaaggt cgagcaggtg accggcctgc cgatgctcca
     3901 gatcgaggtg gaccgcgacg cgatcagccg ctatg
//
LOCUS       DAOSCV010000371         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14589_length_3924_cov_3.308865, whole genome shotgun sequence.
ACCESSION   DAOSCV010000371 DAOSCV010000000
VERSION     DAOSCV010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14589_length_3924_cov_3.308865"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLY56_09915"
     CDS             <1..285
                     /locus_tag="PLY56_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ21839.1"
                     /translation="GRAVLSNHPQTAVAAEEDLLGLSGNAFGPCRSALAVPVPVGAAP
                     AGCLVACRPDPVAFTREEMALVSTMASEVGIAIQALQRQSPKGGQEALWT"
     gene            complement(417..794)
                     /locus_tag="PLY56_09920"
     CDS             complement(417..794)
                     /locus_tag="PLY56_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21840.1"
                     /translation="MAKILVVDDEQRVVDLVAVNLRREGYTVVSARNGGDALVAVGKE
                     KPDLVILDVVMPVMDGVHVAQALKANEATRDLPIVMLTAKAQDADILEGLLAGAEVYL
                     PKPFHPRDLIRVVKSLLPDPSRE"
     gene            complement(817..2643)
                     /locus_tag="PLY56_09925"
     CDS             complement(817..2643)
                     /locus_tag="PLY56_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ21841.1"
                     /translation="MIRRVSRNGRELFVRPLRLTDTDRILSFMVSLSPESRRLFRPHP
                     FNRDDAERAVRDAQTGRSSRFVLVDPQEDEIVGYAYFSESGVGDTRVPMLGIAVADAY
                     QNMGLGRLLMELLFEEARAQGKAGLQLTVYKENARAIHLYTALGFRIVGEADGGKQHA
                     MRVEFAPNTTLVERRGVFLNPMPWGLAPLTADTWSTTEWTAYLDFLHAAGANLLMTFV
                     WPGQYYHPDEASTVRNGWRYGVLRNALEYARGLGMQTLVGFPFTGVPACIWHRHPQQR
                     ATEVGFQGQALCWSRGRDSIQRFARFLLTALADCTDGFVLGIAGPAFCGCSGCRDYAR
                     VVSDAVGHYEGWLSGRSALHIDLTGARELESRIGGAQWEALLRELPKERAALVLPEDR
                     TTSDHLGRAGIPILLCDPGLAGTGGSDLEALIPRPRLRQVDRLVAGNSGAAGVLGQRI
                     MPHTQFVTDFVLMQKLLFPARPNHEILRDLGCHFYARTSHMFEFARAIWALDAWWETG
                     RRGDLLDARDGLMALPADPGGWVRALRDAVDLLFELVRYLEGGERHFDALVTRVHTRM
                     AKSPTFQGCTVDHAWQARARAAVGQHVAGCLDRLRDHLSKLA"
     gene            3205..>3924
                     /locus_tag="PLY56_09930"
     CDS             3205..>3924
                     /locus_tag="PLY56_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21842.1"
                     /translation="MAATSGIASMYAFPWDFHDEGVEKTLATLADKAGMTEVAVACVG
                     IAGTYFLPHNPKRMFYFGEEGALLFKPSANPYQGTRLTAQVAGVVESAEYLAGIAAAC
                     REVGLEFTIWMRYCDNPALGRAFPEYTRQDALGIHHLSQLCPAEPDVRVFCREVTGDL
                     CRQCAPDGLHIEAASFLPYDRGFIHPAAPAPIASFDRLLLGLCFCPLCVAAADAAGDK
                     ITIEPWDYSYYAEKVRTAKYDI"
BASE COUNT          697 a         1320 c         1258 g          649 t
ORIGIN      
        1 ggacgggcgg tgctcagcaa ccaccctcag acggcggttg ctgcggagga ggaccttcta
       61 gggctctccg gcaacgcgtt cgggccatgc cggagcgcgt tggcggtgcc ggtgccggtg
      121 ggcgccgcgc cggccggctg cctggttgcc tgccggccgg atcccgttgc tttcacccga
      181 gaggagatgg cactggtcag cacgatggcc agcgaggtgg gaatagcgat acaggctctg
      241 cagcggcaat cgccaaaggg ggggcaggaa gcgctctgga cctaaccagt cccgcccgga
      301 gaacgcatgg cgatgcgacc gcgtgccctg cgcgttccgt ccgccctgac gactgaaatg
      361 ctgggccacg cgcgatggtg cctggcgcga gcaacgccgg gctcctggac gttcccctac
      421 tcccgggaag gatcagggag gaggctcttg accacgcgaa tcaggtcgcg agggtggaat
      481 ggcttcggca ggtagacttc ggcgccggcc agcaggccct cgaggatatc ggcatcttgc
      541 gctttggcgg tcagcatcac gatggggaga tcgcgtgttg cctcgttcgc tttcagcgcc
      601 tgggccacat gaacgccatc catcaccggc atgacgacat ccaggatcac gaggtcgggt
      661 ttctctttgc cgactgccac gagggcatcc ccgccattcc tggcggatac gaccgtgtag
      721 ccctcccggc gcaggttgac ggcaaccaga tccactacgc gctgttcatc atcgacgacg
      781 agaatcttag ccatggctcc tccaccaccg cgcgacttac gccagcttgc tgaggtgatc
      841 gcgcaatcgg tccagacaac cggcgacgtg ctggccgacg gcggcgcgtg cgcgcgcctg
      901 ccatgcatgg tccaccgtgc agccctggaa cgtgggcgac ttggccatgc gcgtgtgtac
      961 tcgcgtcacg agggcatcga agtgccgctc cccgccctcc agatagcgga ccagctcgaa
     1021 gagtagatcc acggcatcgc ggagcgcgcg cacccagccg cccggatccg ccggcagcgc
     1081 catcagccca tcgcgtgcgt cgagaagatc gccgcgcctg cccgtctccc accacgcatc
     1141 gagggcccag atcgcgcgcg caaactcgaa catgtgagag gtgcgcgcgt agaaatggca
     1201 ccctagatca cgcaggattt cgtgattcgg ccgtgcgggg aagagcagct tctgcatcag
     1261 gacgaaatcg gtgacgaact gggtgtgcgg catgatccgt tggcccagaa cccccgctgc
     1321 tccggagttc cccgccacga ggcgatcaac ctgcctcagg cgcggccttg ggataagcgc
     1381 ctcgagatcg ctccctcccg tgcctgccag accgggatca cacagcagga tcggaatgcc
     1441 tgctctgccc aggtgatcgc ttgtggtgcg gtcttccggc aaaacaagag ccgcgcgctc
     1501 cttcgggagc tcgcgcagga gcgcctccca ctgcgccccg ccgatccggc tttccagttc
     1561 cctcgcgccg gtcaggtcga tatgcagcgc gctgcgcccg gagagccatc cctcgtagtg
     1621 gccaaccgcg tctgatacca cgcgcgcgta gtcccggcag ccggagcagc cgcagaacgc
     1681 cggcccggcg attccgagga cgaagccatc ggtgcaatca gccagggcag tcagcaagaa
     1741 gcgcgcgaag cgctggatcg agtcgcgccc ccggctccaa cacagcgcct gcccctgaaa
     1801 tcctacttca gtggcgcgct gctgcggatg ccgatgccag atgcacgcgg gcaccccggt
     1861 gaagggaaag ccgacgagcg tctgcatgcc caggccacgg gcatactcca gcgcgttccg
     1921 caggacgccg tagcgccacc cgttgcgcac cgtggaggct tcatccgggt ggtagtactg
     1981 cccgggccag acgaaagtca tcagcagatt ggcgcccgcg gcgtgcagga agtcgagata
     2041 ggccgtccac tcggtggtgc tccaggtatc ggccgtgagc ggagccaggc cccagggcat
     2101 cgggttcagg aagacgccgc ggcgctcgac cagcgtcgtg ttcggggcaa actcgacgcg
     2161 catcgcgtgc tgcttgcccc catcggcctc ccccacgatg cgaaacccca gggcggtgta
     2221 gaggtgaatg gcgcgggcgt tctctttata gacggtgagt tgcagaccgg ccttgccctg
     2281 cgcgcgcgcc tcctcgaaca gcagctccat caacagccgg cccagcccca tgttctggta
     2341 cgcatcggcg acagcgatcc cgagcatcgg cacacgcgtg tcgccaacgc ccgactcgct
     2401 gaagtaggcg tagccgacaa tctcatcctc ctggggatcg accagaacaa agcggctgga
     2461 gcgtccggtt tgtgcgtcgc gcacggcacg ctcggcatca tcgcggttga aggggtgggg
     2521 ccgaaagagg cggcgtgact ccggcgagag ggaaaccata aaggagagga tgcggtccgt
     2581 atctgtcagg cgcaaaggcc ggacgaacaa ctcgcgcccg tttcggctga ctctcctgat
     2641 cattccgcgt gtctctccag ccgggcaagc gcccacgccc tggattatac cgcacgtctc
     2701 ctaccccgtc aaggcacgct gagagagcct cgcgcggggc atgcccagac ctcgacgggc
     2761 ttctccttca cggccccgcg agtagccatg tctcggccct gaagggggcg cgcaacaaag
     2821 cccgaggtgt ccgcaccctg agccatgccc cctgaaaagt aaccacgatc ctgaaggggt
     2881 cacggaaacc gctatcacgt attatcgaac cctttcaggg ttccaggtat tttctggatg
     2941 cgagacccag gggcaggaga ccctgggttg ggttgtcgga ccgccacgcg gccctccgag
     3001 agcgtttcgc tcgggccatg cccgatcccg gatggatcac acacccccag gtgcccccac
     3061 ctctccattg gtgttcgtcc cttccatcgg tatccccgga ggaatgagcc ggcgccggaa
     3121 tgggtaagat cgccgcaacg agtctctggc gggccaaacc gaaccgcgcg agacggtggc
     3181 cccccgaggg ggatgggagc gagcatggcg gctacgagtg gcatcgcttc gatgtacgct
     3241 tttccctggg acttccacga tgaaggcgtc gaaaagacgc tcgcaacgct ggcggataag
     3301 gctggcatga cagaagtggc cgtcgcctgc gttggcatcg ctggcaccta ttttctcccc
     3361 cacaacccaa agcgtatgtt ctactttggc gaggaaggag cgcttctctt caagccatcc
     3421 gccaatccgt atcagggaac gcgcctgacg gcgcaggtgg ctggggtcgt cgagtcagca
     3481 gagtacctgg ccggaatcgc ggcggcgtgc cgcgaggtcg gcctcgagtt taccatctgg
     3541 atgcgctatt gcgacaaccc agccctgggg cgcgcctttc cagagtacac ccggcaagac
     3601 gccctgggga tccaccacct ctcgcaactc tgcccggccg agcccgatgt ccgcgtcttc
     3661 tgccgcgagg tgactggcga cctgtgccgc cagtgcgcgc cggacggact gcatatcgag
     3721 gcggcgagct tcttgcccta cgaccggggc ttcatccatc ctgcggcgcc cgcgcccatc
     3781 gcctcgtttg accggctgct gctgggcctc tgcttctgcc cgctctgtgt tgcggccgcc
     3841 gatgcggcgg gcgacaagat caccatcgag ccctgggact acagctatta cgcggagaaa
     3901 gttcgcactg ccaaatatga catc
//
LOCUS       DAOSCV010000372         3913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14640_length_3913_cov_3.119492, whole genome shotgun sequence.
ACCESSION   DAOSCV010000372 DAOSCV010000000
VERSION     DAOSCV010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_3913_cov_3.119492"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    574..583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <584..1850
                     /locus_tag="PLY56_09935"
     CDS             <584..1850
                     /locus_tag="PLY56_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ21843.1"
                     /translation="NEPPWMASNKRWALALLVVLLALLAAGCTRQKAESDQVTLVLAY
                     PADQKTRPLYRETIARFERKHPGIRVRLLEIPKDYYRKVLVMIAGRNAPDLMWMGQSF
                     AEFATRGAFLDITDRVARELNLEEYLPQAIQWYRIEGKQLGIPFGVDMEFIAYNKKLF
                     DEAGVDYPRDDWTLEEFLEKAKRLTKDRNGDGRIDQYGFRGGLDCSAFGAQILAEDGS
                     RACCNTPQMVRHLQFHLDLAHKWRVAPLPDAIQQEGLDIYAAFRQGRAAMMRMYTWDL
                     PALREQCREIDWDIVNGPKVVQRGYWASSQAVLISSETRHPDEAWLLCKEFFGPEFQR
                     SMAQRGLPTNLKIAREVIAANRERPANLAALLKASDALYPFPRVAHLSELLQHWWNAN
                     ESVNCLRATPEVAVARAERAINRAIARGR"
     gene            1855..2781
                     /locus_tag="PLY56_09940"
     CDS             1855..2781
                     /locus_tag="PLY56_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ21844.1"
                     /translation="MRKKGTPVKWRENLTALAFLSPNLIGFLIFTLLPVLAGFLLSLC
                     HWDLFHPPRFVGLRNFVDLLGWYTDEGKVHANDPRFWKYLGNTFFLMMGIPINMAASL
                     LLAMVLNQKLVGRTFFRTIFFLPTICMGVGVMLLWMWILNPEFGLLNYLLSLVNIQGP
                     AWLKSYAWAKPSLMMMQLWVAMGGTSMILYLAGLQGIPPELYEAADVDGASGWDRFRH
                     ITLPLLTPTTFFIFITSVIGGFQGGFDAAYIMTRGGPDGATTTISYYIFSHAFEGFNM
                     GYAAAIAVVLFGLVLVVTLINWRYGGKRVHYV"
     assembly_gap    2938..2947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2948..3628
                     /locus_tag="PLY56_09945"
     CDS             <2948..3628
                     /locus_tag="PLY56_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ21845.1"
                     /translation="PDRLHWENYHKVWVEIDFARALVNSTFVTVAVTAGQVFTSSLAA
                     FAFARLRFFGRDKIFLGYLATLMVPSTVTMIPVFILLRELCWINSYYALIIPAMFSAY
                     GTFLLRQFFLSIPPDLEDAAVIDGCSSFGIYRNVILPLSKPALSALAILTFIGSWRSF
                     MWPLIVTHEKEMFTLPVALASFQEMFTVQWTLLMAGSVIMILPMLLVFIVGQRFFIEG
                     IRVGAVKG"
     gene            complement(3636..>3913)
                     /locus_tag="PLY56_09950"
     CDS             complement(3636..>3913)
                     /locus_tag="PLY56_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21846.1"
                     /translation="GGLLLFGAFWFFVTFAVAHGTRLVRTLQTPYFKAVALLVTLLVC
                     MQMLVSYGDQQLTEYRTMVYLGLLLGTMVRLPAIERQEALQGGSASK"
BASE COUNT          668 a         1294 c         1192 g          739 t
ORIGIN      
        1 cagcgtgccg cgcaggatgc gaaatccgag cacgatcatc gccttcgcat ctgggaagat
       61 gtagcgcggg tccatctgct tcggcgcgcc ctcgaagcgg tccatcgagg cgactccgac
      121 aatatccgcg ccgcactcct tcgcccactt cttcacctga tctgacgtca acatgaaacc
      181 cctccaggga atggcacaag gcagcaccct ggcgctgccc gtgcccccgt gttcggttcc
      241 tggaccggcc tctccttcct catctctctc cgaatcccca ccgcgcggcg ataccacgga
      301 tgaagggggc ggacacggat gggcaggtga ggcgcgaggg atggggcgct ccatctcagg
      361 gggctacgcc aaacgcgcgt gccacggggg tgtggcaagc acgatctctc gggaggaacc
      421 tgcccgcgag cagcaaactt ctgaccaact tctgtctcgg atagcgccgc ggccccgaca
      481 gccgccggcg cgcgctcgga gcagaggatc gcctctgcag ggggctaccg cctatgcttc
      541 tatgcaagag acgcacgcgc tgcgaggcca gggnnnnnnn nnncaatgaa ccgccgtgga
      601 tggcatccaa caagcgctgg gcactggcat tgctggtggt gctcctggcc ctgctggctg
      661 ccggctgcac ccgtcagaag gcagagagcg accaggttac cctcgtgttg gcctatccag
      721 cggaccagaa gacgcggcca ctctaccgcg agacgatagc gcgcttcgag cgcaagcatc
      781 cgggaatccg cgtgcgcctg ctggagatcc ccaaggatta ctaccgcaaa gtcctcgtca
      841 tgattgccgg gcgcaacgcg ccggacctga tgtggatggg ccagagcttc gccgagtttg
      901 ccacccgcgg cgccttcctc gatatcaccg accgagtggc gcgcgagttg aatctcgagg
      961 agtacctccc gcaggcgatt cagtggtacc gcatcgaggg gaagcaactc ggaatcccct
     1021 tcggtgtgga catggagttc atcgcctaca acaagaagct cttcgacgaa gccggcgtcg
     1081 actatccgcg cgacgattgg accctcgagg agttcctgga gaaggcaaag cggctcacca
     1141 aagacaggaa cggcgacggg cgcatcgacc agtatggctt ccgcggcggt ctcgactgct
     1201 ctgcgttcgg ggcgcagatc ctcgcggagg atggttcccg cgcgtgctgc aacacgccgc
     1261 agatggtgcg ccacctgcag tttcacctcg acctggcgca taaatggagg gtggcccccc
     1321 tgccggatgc catccagcag gaggggctcg acatctacgc cgcgttccgc cagggacgcg
     1381 cggcgatgat gcgcatgtac acctgggatc tgcccgcgct gcgagagcag tgccgcgaga
     1441 tcgattggga tatcgtcaac ggcccgaagg tggtccagcg cggctactgg gcatccagcc
     1501 aggcggtgct catctcctcg gagacgcgcc atcccgacga ggcgtggctc ctctgcaagg
     1561 agttttttgg cccggagttc cagcggagca tggcgcaacg cggcctcccc acgaacttga
     1621 agatcgcgcg ggaggtgatc gcggctaacc gcgagcgccc ggccaacctg gccgccctgc
     1681 tgaaggcaag cgacgccctc tatcccttcc ctcgggtggc gcacctctcg gagctgctgc
     1741 agcattggtg gaacgccaac gagagcgtca actgcctgcg cgccacgcct gaagtggccg
     1801 tcgcacgcgc ggaaagggcg atcaaccgcg caatcgcccg ggggaggtag gcgcatgagg
     1861 aaaaagggca cgccagtcaa gtggcgcgag aacctgacgg cgctcgcgtt cctgtcgccc
     1921 aacctgatcg ggttcctcat cttcaccctt ctgccggtgc tggccggctt tttgctcagc
     1981 ctctgccatt gggacctctt ccatcccccg cgcttcgtgg gccttcgcaa cttcgtggac
     2041 ctgctcgggt ggtacaccga cgagggcaag gttcacgcga acgacccgcg cttctggaag
     2101 tacctgggca acacgttctt cctaatgatg gggattccga tcaacatggc ggcatcgctg
     2161 ctgctcgcga tggtgctcaa ccagaagctg gtcgggcgca cgttcttccg aaccatcttc
     2221 ttcctgccga cgatttgcat gggcgtgggc gtgatgctcc tctggatgtg gatcctcaac
     2281 ccggagtttg gcctgctgaa ctaccttctc tcgctggtga acatccaggg tccggcgtgg
     2341 ctgaagagct atgcctgggc caagccatcc ctgatgatga tgcagctctg ggtggcgatg
     2401 ggcggcacaa gcatgatcct gtatctcgcc ggcctgcagg ggatcccccc ggagctgtac
     2461 gaggccgccg atgtggatgg cgcgagcggc tgggatcgct tccggcacat cactctcccg
     2521 ctgctaacgc ccaccacctt cttcatcttc atcacctccg tcatcggtgg cttccagggc
     2581 ggcttcgacg cggcctatat tatgacgcgc ggcggccctg atggcgcgac cacgaccatc
     2641 agctactaca tcttcagcca cgccttcgag gggttcaaca tgggttacgc ggccgcgata
     2701 gcggtcgtcc tcttcggctt ggtgctggtc gtcactctca tcaactggag atacggggga
     2761 aagcgggtcc actatgtcta gtctctcgcg caccacttcc cggcgcctgg cgcacgggct
     2821 ctcttatctc ttcctgagcg tggtgggcat cagcatggtc gtgcctctcc tctggatggt
     2881 cgcgacctcg ctgaaatccc ccactgccga tgttctcgac ccggcgcaat ggcttccnnn
     2941 nnnnnnncca gaccgcctgc actgggagaa ctaccacaag gtgtgggtgg agatcgattt
     3001 cgcgcgcgca ctggtgaaca gcacctttgt cacggtcgcg gtcaccgccg gccaggtgtt
     3061 caccagctcg ctcgccgcgt tcgcgttcgc gcgccttcgc ttcttcgggc gcgataagat
     3121 cttcctcggc tacctcgcca cgctgatggt cccctccacc gtgacgatga tcccggtctt
     3181 catcctgttg cgcgagctct gctggatcaa ctcctactat gccctgatca tccccgcgat
     3241 gttcagcgcc tacggcacct tcctcctccg gcagttcttc ctcagcatcc cgcccgacct
     3301 ggaggacgcg gcagtgatcg acggctgctc ctcattcggc atctatcgga atgtaattct
     3361 cccgctctcc aagccggcgc tctccgccct cgccatcctc accttcatcg ggtcgtggcg
     3421 cagcttcatg tggccgctca tcgtgacgca cgagaaggag atgttcacgc ttccggtggc
     3481 gctcgcgtcg ttccaggaga tgttcaccgt tcagtggacg ctgctgatgg ccggctccgt
     3541 gatcatgatc ctcccgatgc tcctcgtctt catcgtcggc caacgcttct tcatcgaggg
     3601 catccgcgtt ggcgctgtga agggctaggg cgcggctact tggaggcgct ccctccctgg
     3661 agtgcctcct gccgctcgat ggccggcaga cgaaccatcg tacccaggag caggcccagg
     3721 tagaccatag tgcggtactc cgtgagctgc tggtcgccgt aggagacgag catctgcatg
     3781 cagacgagaa gcgtcaccag cagggcgacc gccttgaagt agggcgtctg cagcgtgcgt
     3841 accaggcggg tgccgtgggc gacggcgaag gtgacgaaga accagaacgc tccgaagagc
     3901 agcagccccc cca
//
LOCUS       DAOSCV010000373         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14667_length_3907_cov_2.708723, whole genome shotgun sequence.
ACCESSION   DAOSCV010000373 DAOSCV010000000
VERSION     DAOSCV010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14667_length_3907_cov_2.708723"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="PLY56_09955"
     CDS             complement(<1..1461)
                     /locus_tag="PLY56_09955"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOJ21847.1"
                     /translation="MDKRQIRNAVAALAITAAAVALLWAAGRLHVTAVVLTKLEYTAC
                     DTLLFGGRGPLPTPEEVVVVAIDPPSVQNLGQWPFPRSYHARLVNQLAAMGAKVVAFD
                     IVMDVGRGDQDDRELVDACRRAGNVIMAAQVDSSEDPIGGHRRMMLVMPFEELREVCA
                     LGTVNFTKEEDSKVRFASAGHEVAGVQIPLLSVHAAASFLGVDAERLVTGNTLHVGET
                     RIPLDSSGRFLINYVGKQKDVRTFSYYQFLPPPGESIGVPAEHIRDKVVFVGVTDPLE
                     QDVVDIPYENTFPGVTVHANIASMLIRNQPIRRLPAGFGLLFAAVFAAGLSAASVSRR
                     PQWAAISLVALITAYSMVAASLFSSAGIWIPVVVPLLGAALATIGGIIYGYLVEGRER
                     RRISSLFSKYVSRDVARELMEDEGAAQLGTSRKLPVTCLFSDIRGFTSISERLSPEEV
                     VAMLNQYFDRMVECVFENRGTLDKYVGDAIMATFGVP"
     gene            complement(1984..>3907)
                     /locus_tag="PLY56_09960"
     CDS             complement(1984..>3907)
                     /locus_tag="PLY56_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HOJ21848.1"
                     /translation="EGPYDDLWKLEERGKPPKEEERPRRRIPEEPEKDILGFLIQHAP
                     ELDDWQRDVISIVREEMLYFLPQMQTKIMNEGWACATGDALLATSRGFIRFRDLYEQE
                     MRITIGSGEPGALHPITAFHKEEGVPTLRITTRRGYTLEGALKHRVRLADGSWAFLRD
                     LRQGDRVALARGIEVWAAEEVPIEYQPETPGTIGGPQARPPATLNAPLAYLLGYFTGA
                     GTVTESGVSFTCDDEAHARYLGDLVETALGVPAPVREDGAPTRKRWCIELSSREVARL
                     FETLGAGAGARDVPDAILRSPRHIVSAFLRGCFDASGCPGAEGVTLSTRSDELVQCAQ
                     ILLLNYGILSARSVQADGSARLEITGSSAALFRDAIGHELPCKRAALDAYLEGCSGSQ
                     QEEATDEIVAIEPGNADVFDITVDVAHAYVANGLINHNSFWHGRIMRELDLTDDEFIE
                     YSQLNAGVLSPSPSRRQINPYYVGVKIWEDILRRWDSPTEEERKLFGREPGKGMEKLF
                     EVRELDNDVSFLRNYLTEELVEELDLYVYELRDDEWVITDKDWEKTRDMLVASMTNMG
                     YPVIEVHDGDYRRNRELYLVHAYEGRELDIAYAEKALQHVYTLWGRPVHLETVVDGEP
                     VLLTYDGHDNITRVLN"
BASE COUNT          713 a         1341 c         1196 g          657 t
ORIGIN      
        1 cggcacgccg aaagtggcca tgatcgcgtc gccgacgtac ttgtccagcg tgccgcggtt
       61 ctcgaagacg cactccacca tcctatcgaa gtactggttc agcatggcga ccacctcctc
      121 gggagacaat cgctcgctga tgctggtgaa gccgcgaata tcggagaaga ggcaggtcac
      181 cggcagcttg cgactggtgc cgagctgcgc cgcgccctca tcctccatca gttcccgcgc
      241 cacgtcgcgc gagacgtact tgctgaagag cgaactaata cgccgacgct ctcgcccctc
      301 cacaaggtag ccatagatga taccgccgat agtagccagc gccgcgccca gcaggggaac
      361 gaccaccgga atccagatcc cggcactcga gaagaggctg gcagccacca tggagtaggc
      421 ggtgatcagc gccaccagac tgatggcagc ccactggggc cgacgcgaca cgctcgctgc
      481 gctcaagcca gcagcgaaca ccgcagcgaa gaggagtcca aaaccagcgg gcagccgccg
      541 gatcggctgg tttcggatca gcatgctcgc gatgttcgca tggacggtga ccccggggaa
      601 ggtgttctcg taggggatat ccaccacgtc ctgctccagg ggatccgtca ccccaacgaa
      661 gactacctta tcccggatat gctcggccgg cactccgatg gactccccgg gcgggggcaa
      721 gaactgataa taggagaagg ttcgcacatc cttctgcttg cccacgtagt tgatcaggaa
      781 gcgccccgat gaatcgagcg ggatccgcgt ctcgcccacg tgcaaggtgt ttccggtgac
      841 aagcctctcg gcgtctacgc ccaggaagct cgccgcggca tggaccgaga ggagcgggat
      901 ctgcacgccg gccacttcat gaccggcgct tgcgaaccgc accttgctgt cttcctcctt
      961 cgtgaagttc accgtgccga gggcgcacac ttcgcgaagc tcttcgaagg gcatcacaag
     1021 catcatccgc cggtgccccc cgatgggatc ctcactggag tccacctggg cagccatgat
     1081 cacgttcccg gcacggcggc aggcgtccac cagctcgcga tcatcctggt cgccacggcc
     1141 aacatccatg acgatatcga aggcgaccac cttcgcgccc atcgccgcca gctggttcac
     1201 aaggcgagcg tggtaggagc gcgggaaagg ccactgaccc agattctgca cagagggggg
     1261 atcaatggcg acgacgacca cttcctcggg cgtagggagc gggccccgcc ccccgaacag
     1321 gagtgtatca cacgcggtgt actctagctt cgtcaagacc accgccgtta cgtgcagccg
     1381 tcctgctgcc cacaacagcg ccacggcagc tgccgtgatc gccagggctg caaccgcgtt
     1441 cctgatctgg cgcttgtcca tgaggcagta ccttccgatt gcgaggcgcc atcgatgccg
     1501 ccgacaatcg cccggacact agccccgcgg gcgtctcatg agggggcgcg accgccagca
     1561 agggaagtgt gttgggtgag agcggggaat gcacgctccc gggcgtaccg ccagccaggg
     1621 acgaggtgac gctcgctcgc tactcgctat tatcggatcc ctgccgcaga tcttgaggga
     1681 caacgggatg aacgggctgc gcgccggtgg cggcaccgtt gataccacgg atgaaagggg
     1741 cacacgcaga taggccgggg tatcgacggt gccgcgcgcc gccttctccc attcctcttc
     1801 cgtgggcagc cggcagcccg cctaccgcgc ggaggccatc gcgtcgtccc aggagacgcc
     1861 cacgaccggc aggcggccat ccgtgccatt ccaccggcca caccggcatc gatgccgtcg
     1921 atgggacagc ggcagccccg cggggctcct cccgcggggc tgcaaatacg ggctctctcc
     1981 agcttagttc aggacgcgcg tgatgttgtc gtgcccatca taggtgagaa gcacaggctc
     2041 gccatcgacc acggtctcga ggtggaccgg ccttccccac agcgtgtaga cgtgttgcaa
     2101 cgccttctcc gcgtaggcga tatccagctc ccggccctcg taggcatgga ccaggtaaag
     2161 ctcgcgattg cgccggtagt cgccatcatg aacctcgatg accgggtagc ccatattcgt
     2221 catgctggcg acgagcatgt cgcgcgtctt ctcccagtcc ttgtccgtaa tgacccactc
     2281 gtcgtcgcgc agctcgtaga cgtacaggtc cagctcctcc accagctcct cggtcaggta
     2341 gttgcggagg aacgacacgt cgttgtccag ctcgcgaacc tcgaacagct tctccattcc
     2401 cttgcctggc tcgcgaccga acagcttccg ctcttcctcg gtgggcgaat cccaccgacg
     2461 cagaatgtcc tcccagatct tgaccccgac gtaatagggg ttgatctgcc ggcgcgacgg
     2521 cgaaggcgag agaacgcccg cgttgagctg cgaatactcg atgaactcgt catcggtcag
     2581 gtcgagctca cgcatgatgc gcccgtgcca gaaagagttg tggttgatca ggccattggc
     2641 aacgtaggcg tgggccacat ccaccgtgat atcgaagacg tcggcgtttc ccggctcgat
     2701 cgccacgatc tcgtcggtcg cctcctcctg ctgggaaccg ctgcagccct ctaggtaggc
     2761 atccaatgca gcgcgcttgc acggaagctc gtgcccgatg gcatcacgga acagagcggc
     2821 agaggacccc gtgatctcca ggcgcgcgct tccatctgcc tggacgctcc gggcactcaa
     2881 gatcccgtag ttcaagagaa ggatctgcgc gcactgcacc agctcgtcac ttcgagtgga
     2941 gagcgtcacg ccctccgcgc ccgggcaccc actcgcatcg aagcagcctc tcaggaacgc
     3001 cgagacgata tgccgcggcg agcgcaggat cgcgtccggc acatccctgg caccggcgcc
     3061 cgctcccagc gtctcgaaga ggcgcgccac ctctcgagaa gagagctcaa tgcaccagcg
     3121 tttccgggta ggcgctccat cctctcgcac gggcgccggc acgccgaggg ccgtctcgac
     3181 caggtcgccc aggtagcggg cgtgcgcctc atcgtcgcag gtgaacgaca cgcccgactc
     3241 cgtcaccgtg ccggcgcccg tgaagtagcc gagcaagtag gccagcggcg cattgagcgt
     3301 tgccggcggt cttgcctgcg ggcccccgat ggtgccgggc gtctctggct ggtactcgat
     3361 gggcacctct tccgcagccc agacctcaat gccgcgcgcc agcgccaccc ggtcaccctg
     3421 cctgagatca cgcaggaacg cccacgaacc atcggccaga cgcacgcgat gcttcagggc
     3481 gccttccaac gtgtagcccc ggcgcgtggt gatccgcagc gtcggcaccc cctcctcctt
     3541 gtggaaggcc gtgatcggat ggagcgcgcc aggctccccg ctgccgatcg tgatccgcat
     3601 ctcttgctca tacagatcgc ggaagcggat gaagccgcgc gaggtggcga ggagcgcatc
     3661 cccggtcgca cacgcccagc cctcgttcat gatcttcgtc tgcatctgcg ggaggaagta
     3721 gagcatctcc tcgcgcacga tgctgatcac gtcgcgctgc cagtcatcta gctcgggggc
     3781 gtgctggatg aggaagccga ggatatcctt ctccggctcc tcggggatgc gccggcgggg
     3841 ccgctcctcc tctttcggag gcttgccgcg ctcctccagc ttccagaggt catcataggg
     3901 tccctcg
//
LOCUS       DAOSCV010000374         3905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14676_length_3905_cov_3.237143, whole genome shotgun sequence.
ACCESSION   DAOSCV010000374 DAOSCV010000000
VERSION     DAOSCV010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14676_length_3905_cov_3.237143"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /gene="pilM"
                     /locus_tag="PLY56_09965"
     CDS             <1..1157
                     /gene="pilM"
                     /locus_tag="PLY56_09965"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOJ21849.1"
                     /translation="PPETLSGQEVLDPLRLGHALRNLWRRIGLGEHAASVAVLAPSYG
                     LRALRMPQAPERERNALVRGELEQAQVIPQERGAFDFLWIASGEPGVPQADVYAYHIN
                     ETIIDGLHRSFHGAGLRLNSVEPGSIALMRLYLATRGKRDPVAILCPSARHTDLCIHD
                     GTRVRQLRRIPAGWEDLGTTLERGTRDIIPEPRIWDAHVPSEKRGGMLNLSEGESLPE
                     EAPDAEGTLANEASPPAEPTFQGIPNTWPIRSEFSDEVPDILSIGAGQSPVGGQSAHK
                     SDFLVSEISRTLAFFEREHPEEAHPQEIVVLGPTDAARGFERVLSPAQSLPIVVANPL
                     AGLDLPRAVAVPGLEEDPEAYALAVGVALAASGVDLGIPPVDLSRQTSVA"
     gene            1194..1670
                     /locus_tag="PLY56_09970"
     CDS             1194..1670
                     /locus_tag="PLY56_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21850.1"
                     /translation="MAWSTLWLLASAIAAIGLMIAEARLAETHVQLTNEIAKIHAQRA
                     PLIRAAELSKAAESARQLSEIPAAAVLGRVAAATTTGVGLTSLRISPDGKVSLEGKAL
                     GTSNIQRFALSVGRGKSVRVPVIETTKRDTHGYVTFRIGALFREPAPPPPPATGKE"
     gene            1676..2239
                     /locus_tag="PLY56_09975"
     CDS             1676..2239
                     /locus_tag="PLY56_09975"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HOJ21851.1"
                     /translation="MRGITLSPDLQQLMAVGVPIAALLISLGVVYPGYQRYVEMSAKV
                     EKQRQELHKLKNTPLPPVSRALPAADPEPGESARFLGHLVSLATSANCRFVGLDSAPV
                     RVAHSGGLIRAVRTRVEIEGRYPQIRQFLAELANASRIYVVTSLDIDTRGRTNQPGQA
                     PSTVRAIVEIERYLTVEPETTNVAASR"
     gene            2297..>3905
                     /locus_tag="PLY56_09980"
     CDS             2297..>3905
                     /locus_tag="PLY56_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ21852.1"
                     /translation="MNKRDGVIPNTQTMRAVALRAGTRTAGCTLWAVIACAWLAPPGA
                     ASPDARASAPLRTHVVRAGEHLSKIAKEYGVPVKALAEANGIAHPDRVPPGKKLVIPG
                     AASAEVATPAAKPAKRIEVADVDPDYRPPTRSSKPKARAPGKNASKQTKPQVPITSRG
                     GSNNNPARGTAAGAVPQSGAGTATEAPHTRQLEPPLAQRRAPDPNARISLNFADVPAR
                     DALAAISAHTGVDTLITPGTTGNVSINLRDRTADEAIRQVAAVAGLSVAQIGGTYVVG
                     PAAEVQKAVAELGKTEVVTLRHITPDQAKEVLARAVPRLNVETAGKALILSGLPEDLE
                     AARTALEGIDAAAPAAPSSKTEFALLRHADPEMVERMVCEAFPNLKVTRQDRLFTMTG
                     SPADLASASQAIKMLDAPPPAAQVAATPTPQRETFIYELRYTNPQRAEEALKKLLPDL
                     VVASAPEATAPPAATFQPLSLGMMGGTNGSGFTFSSTGVGTGSGTSGPYAQPLARSTR
                     LVLMGTPADIATARQILEETDVAPPMVR"
BASE COUNT          699 a         1336 c         1284 g          586 t
ORIGIN      
        1 cgccaccgga aacgctctcc ggccaggagg tgctcgaccc gctccgcctg ggacacgccc
       61 tgcgcaacct ctggcggcga atcggcctgg gagagcacgc cgcatcggtc gccgtgctgg
      121 cgccgtccta cgggctacgc gcgctgcgaa tgccccaggc tcccgagcgg gagcgcaacg
      181 ccctggtgcg cggcgaattg gagcaggcgc aggtgatccc ccaggagagg ggggcgttcg
      241 acttcctttg gattgcctcc ggggaaccag gcgttccgca agccgatgtc tacgcctatc
      301 acatcaacga gaccatcatc gacggtctgc atcggtcatt ccatggggcc gggctgagac
      361 tgaattcggt cgagccaggt tccattgccc tgatgcgcct ctacctggca acccgcggca
      421 agcgcgatcc ggttgccatt ctctgcccct cagcgcggca caccgatctg tgcatccatg
      481 acggcacgcg cgtccggcag ctccgccgga tccccgcagg ttgggaagac ctggggacga
      541 cgctcgagag aggcacgcgc gacatcatcc ccgagccgcg tatctgggat gcccacgtgc
      601 cgagcgaaaa gcgcgggggc atgctcaacc tctctgaggg cgagtcactc ccagaggagg
      661 cgcctgatgc cgaggggacc ctggccaacg aggcctcgcc tccggcggag ccaaccttcc
      721 agggcattcc gaatacctgg ccaattcgca gcgagttctc ggatgaggta cccgatatcc
      781 tctccatcgg tgccgggcag tcgcccgtcg gcggacaatc cgcccacaag agcgacttcc
      841 tcgtttcgga gatctcgcgc acgctcgcgt tcttcgagcg agagcaccca gaggaggcgc
      901 atccccagga gatcgtggtc ttagggccga ccgatgccgc gcgtggcttc gagcgcgtct
      961 tgagcccggc gcagagcctt ccgatcgtgg tcgcgaaccc tcttgcaggg ctcgatctac
     1021 cccgcgccgt cgccgtgccc ggcctggagg aggatccaga agcgtatgcg cttgcagttg
     1081 gcgtggcgct ggcagcctcc ggcgtcgacc tggggatccc cccggtcgat ctctctcgcc
     1141 agaccagcgt ggcgtagccc gggcgccggg tgccacatct cctccgagca gggatggcgt
     1201 ggtcgaccct ctggctgctg gcgtccgcca tcgcggcgat cggcctgatg atcgcggagg
     1261 cccgcctggc cgagacgcac gttcagttga cgaacgagat cgccaagatc cacgctcagc
     1321 gggcgccact catccgcgct gccgaactct cgaaggcggc ggagagcgcg cggcagttgt
     1381 cagagattcc tgccgccgcg gtgctcgggc gtgtcgcggc cgctactacc acaggagttg
     1441 gcctcaccag tctccgcatc tcacccgacg ggaaggtgtc tctggagggg aaggcactgg
     1501 gcacgagcaa catccagcgc ttcgcgctca gcgtgggcag gggcaagagc gtgcgcgttc
     1561 ccgtcatcga gacgaccaag cgagacactc atgggtatgt caccttccgc atcggggctc
     1621 tcttccgaga gccggcgcca ccgcccccgc ccgcgaccgg taaggagtag aggtcatgcg
     1681 aggaattaca ctcagtcccg acctccagca actgatggct gtaggcgtgc cgattgccgc
     1741 cctgctgatc tcgctggggg tcgtctatcc cggctaccag cgctatgtgg agatgagtgc
     1801 caaggtggag aagcagcggc aggagctaca caagctgaaa aacaccccgc tgccccccgt
     1861 gagccgggcg cttcccgcgg cagatcctga gcccggggaa tcggcacgct ttctgggaca
     1921 tctcgtatcg ctggccacaa gcgccaactg tcggttcgtt ggcctggatt cggcgcccgt
     1981 acgggttgcg cactcgggcg gcctcattcg ggctgtgcgg acgcgggtgg agatcgaagg
     2041 aagatacccg cagattcgcc agttcctggc ggagctggcg aatgcctcgc gcatctatgt
     2101 cgtcacatcc ctcgatatcg acacccgggg gcggaccaac cagcctgggc aagcaccatc
     2161 cactgtgcgc gccatcgtcg agatcgagcg ctacctgaca gtcgagcccg agacaacaaa
     2221 cgtagcggca agccgctaag agacacgcat cagtggcatg cctggcaggc agcggaggag
     2281 tgaaaggatg gggcatgtga ataagagaga tggtgtgata ccgaacaccc agacgatgcg
     2341 agcggtggca ctgcgcgccg gcactcgcac tgccggatgc acgctctggg ctgtgatcgc
     2401 ctgcgcgtgg ctcgccccgc caggtgccgc cagcccggac gcgcgcgcat ccgcccccct
     2461 gcggacacac gtcgtccgcg ccggggagca cctctcgaaa atcgccaagg agtatggcgt
     2521 gccagtgaag gctctggccg aggcaaacgg catcgcgcat ccggatcgcg tgccgccagg
     2581 gaagaagctc gtcatccctg gcgccgccag tgcggaggta gccacgccag cggccaagcc
     2641 cgcgaagcga atcgaggtgg ccgacgtgga tccagactat cggccaccca cccggtcatc
     2701 gaagccgaaa gcgcgcgccc ctggcaagaa cgcttcaaaa cagacgaagc cgcaggtgcc
     2761 catcacctcg cgtggtggga gcaacaacaa ccccgctcgg ggcacggccg ccggagcggt
     2821 gcctcaatcc ggagcgggta ccgcgacgga ggcaccccac actcgccagc tggagccgcc
     2881 actggcgcag cggagggcac ctgatccaaa cgcgcgcatc tcgctcaact tcgcggatgt
     2941 gccggcccgc gacgcgctcg ccgcgatctc ggcacatacc ggcgtagaca ccttgatcac
     3001 cccgggcaca accgggaacg tctcgatcaa cctgcgcgat cgcaccgccg atgaggcgat
     3061 ccgacaggtc gcagcggtcg ccggcctttc ggtcgcgcaa attggcggta cgtacgtcgt
     3121 tggcccggcg gccgaggtgc agaaggcagt cgcggagctg gggaagacgg aggtggtgac
     3181 gctccgccac atcacgcccg accaggcgaa ggaggtcctc gcgagagcag tgccacgcct
     3241 caacgtggag actgccggga aggcgctcat cctctccggg cttccggaag atctggaggc
     3301 cgcccggacg gccctcgagg ggattgatgc cgcggctccg gcggcgccct catccaagac
     3361 ggagttcgcg ttgcttcgcc acgccgaccc ggagatggtg gagcgcatgg tgtgcgaagc
     3421 cttccccaat ctgaaggtga cgcgtcagga tcgcctcttc acgatgacgg gctcgccagc
     3481 ggatctggcc agcgcgtcgc aggcgatcaa gatgctggac gccccaccgc cagcggcgca
     3541 ggtggcagcg acaccgacac cgcagcgcga aacgtttatc tatgaactcc gctacaccaa
     3601 cccgcagcgc gccgaggagg cgctcaagaa gctgctgcct gatctggtgg tcgcatccgc
     3661 cccggaagcg accgcgccgc cggcagctac cttccaaccg ctgtcgctcg ggatgatggg
     3721 cggcaccaac ggttccggct tcaccttcag ctccaccggg gtcggcaccg gatcgggaac
     3781 tagcggaccg tatgcccagc cgctggcccg atccacacgc ctggtcctga tgggaacgcc
     3841 cgccgacatc gccaccgcgc ggcagatcct ggaagagacg gatgttgcgc cgccgatggt
     3901 gcgga
//
LOCUS       DAOSCV010000375         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14779_length_3886_cov_3.269903, whole genome shotgun sequence.
ACCESSION   DAOSCV010000375 DAOSCV010000000
VERSION     DAOSCV010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14779_length_3886_cov_3.269903"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PLY56_09985"
     CDS             <1..1107
                     /locus_tag="PLY56_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ21853.1"
                     /translation="FCMEEREAERFRDESQRTYRSGMRIVRINGILRPTMELLGATSV
                     AFILWYGGMEIVQGRLTVAGVTMIFYVALEITKGIQQVGRLRMYVDRVHGAAERIYTV
                     LDMQPEVRNAPDALTLEEVQGDIVFEDVHFAYADGDPVLRGVSFKVRPGEALAVVGPS
                     GAGKSTIANLIPRFYDVTSGSVRVDGHDVRAITTQSLRAHIGIVAQETVLFSGTIREN
                     IAYGRPDATEEEIIEAARAANAHDFISQMLPDGYETVVGERGVKLSGGQRQRIAIARA
                     LLKDPRILILDEATSALDTESEALVQEALDKLMQGRTTIIIAHRLSTVRKADRILVLA
                     QGRIQEQGTHADLLQMNGLYARLYEVGAGLEAVK"
     gene            1228..2586
                     /locus_tag="PLY56_09990"
     CDS             1228..2586
                     /locus_tag="PLY56_09990"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOJ21854.1"
                     /translation="MSVARRWRVMHLLYNTLIILALPALLVYFGYRWLRGKSREGWAE
                     RMGYLPVFTGEPPIWVHAVSAGEAVAAEAVVRALRERLPDVPILYTAGTPEGRATARK
                     RMGSLLTHVAYAPYDLPWAVARAMDRVRPRLYLLVESDLWPNLLQAAAVRGVPAMLAN
                     GYISERTLRRGRLVPALYRWIFGNLCSICVQTEAVRRRALALHAVPERVCVVGNVKYD
                     QATAPLTDEQASRWRALFRLRPDEPVLVAGSTHPGEEEKVLAAYSAMRASGIAPRLIL
                     APRHPQRAEEVETLVRHAGFACVRRSHLGEGDGPAPEPDAVILLDTMGELAGVFSLAT
                     VVFLGGSLVPVGGHDILQPLIAGKPVLLGPYTHRQREIVEDALDAGVVWVVTTPQELA
                     RRSLELMKDAGSGDGYAANAVSFVSGRRGASQRCAEEAARLLGEMQPLRTHGVGKAVG
                     KS"
     gene            2631..3851
                     /gene="lpxK"
                     /locus_tag="PLY56_09995"
     CDS             2631..3851
                     /gene="lpxK"
                     /locus_tag="PLY56_09995"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOJ21855.1"
                     /translation="MRFPFSASLPTPYLLRVADGEATGPLAFGIRAGLRALSWLYLAG
                     VGASVLAHRSGLLRCERAPCPVIGVGNLTVGGTGKSTATAEFARWLLEAGLRPAVLSR
                     GYGVQGSPPVRVVSDGKNVLLGPEEGGDEPVMLAHHLAGVPVLIGRRRPHTARAAVER
                     FSADACLLDDGFQVWNLRKDLEVVLLDSRRPFDNGYVLPRGMLREPPSHLGRADAVVL
                     MEPDRVEREALARLRKQVEALAPGALVAEAGRVPERLWDLATGQDYPLSALNGLPIVA
                     LSAIGNPQGFEDLLGSLGARIYPARLPDHHPYTPDDVEWVQRLAKQKGAAAIITTEKD
                     AVKLDECGGARGRRGEAGAWGRALATEASQESVISAAPDGKAPVLVFSVRLRFTAGGD
                     ALRERVLATVREKR"
BASE COUNT          591 a         1176 c         1413 g          706 t
ORIGIN      
        1 ttctgcatgg aggagcgcga ggcggagcgc ttccgtgatg agagccagcg cacctaccgc
       61 agcggaatgc ggatcgtgcg catcaacggg atcctccggc ccaccatgga gctcttgggc
      121 gccacttcgg tggcgttcat cctctggtat ggcggcatgg agattgtgca ggggcggctg
      181 acggtcgccg gggtcacaat gatcttctac gtcgccctcg agatcaccaa gggaatccag
      241 caggtcggcc ggctgaggat gtatgtcgac cgcgtccacg gcgcggcaga gcggatctac
      301 accgttctgg acatgcagcc ggaagtccgc aatgcccccg acgctctcac cctcgaagag
      361 gtgcagggcg acattgtctt cgaggatgtc cacttcgcgt atgcggatgg cgacccggtg
      421 ctgcggggcg tcagcttcaa ggtgcgccca ggtgaagcgc tcgcggtggt cggtccgagc
      481 ggcgcgggga agtccaccat cgccaatctg atcccccggt tctatgacgt gacctcgggg
      541 agcgtccgtg tcgatgggca tgatgtgcgc gcgatcacga ctcaatcgct gcgcgcgcat
      601 atcggtatcg tcgcacagga aaccgtgctc ttttcgggca ccattcgcga gaatatcgcc
      661 tacgggcgcc cggatgccac ggaagaggag atcatcgagg ccgcccgggc cgcgaacgcg
      721 catgacttca tctcgcagat gctccccgat gggtatgaga cggtggtagg cgagcggggc
      781 gtgaagctct cgggcgggca gcgccagcgc atcgcaatcg cgcgcgctct gctgaaggat
      841 ccccgcattt tgatcctgga cgaggccacc tctgcgctcg acaccgagtc ggaagctctt
      901 gttcaggaag ccctcgacaa gctgatgcag gggcgcacga cgattatcat cgcgcaccgc
      961 ctctccacgg tgcgcaaggc agaccggata ctggtgctcg cgcagggtcg gattcaggag
     1021 cagggcacgc acgccgatct gcttcagatg aatgggctgt atgcccggct ctacgaggtg
     1081 ggcgccgggc tggaagcggt gaagtaagcg cgcgatagcg atgaggtggc tgatggatcc
     1141 tctaacgggc cgccgggagc gccgcgcatc tcctctgcat agaagagcgc ctcgccgcgg
     1201 gatcggggcg tgagacagga gagttcagtg agtgtagcga gacgttggcg ggtgatgcat
     1261 ctgctctata acacgttgat catccttgcg ttgcccgcgt tgctcgtcta ctttggctat
     1321 cggtggttgc ggggcaagtc gcgcgaggga tgggcggagc ggatgggtta cctccccgtc
     1381 ttcacgggcg agcctcctat ctgggtgcat gcggtatcgg ccggcgaggc cgtggcggca
     1441 gaggcggtgg tgcgtgccct gcgcgagcgg ctgcccgacg tgccgatcct ctataccgcg
     1501 ggcacgccgg agggccgggc gacggcgcgt aagcgcatgg gaagcctgct aacccatgtg
     1561 gcgtatgcgc cctacgacct tccctgggcg gtggcccgcg ccatggatcg ggttcgcccg
     1621 cggctttacc tgctggtaga gtcggacctt tggccgaact tgctgcaagc ggcggcggtg
     1681 cgcggggtgc cggcgatgct ggccaatggc tacatctccg agcgaacgct ccggcgcggc
     1741 cgtctggtgc ctgcgctcta ccgctggatc tttggcaatt tgtgctcgat ctgcgtgcag
     1801 acggaggcgg tgcgccggcg cgcgttggcg ctgcatgctg tgccagagcg cgtgtgcgtg
     1861 gtcggcaacg tgaagtatga tcaggcgacc gcgccgctca ctgatgagca ggcgagccgc
     1921 tggcgtgccc tgtttcgctt gcggccggac gagccggtcc tggtggcggg cagcacccac
     1981 ccgggcgaag aggagaaggt cctcgcggcc tactcggcga tgcgcgcgag tgggatcgcg
     2041 ccgcgtctta tcctggcgcc tcggcatccg caacgtgccg aggaagtgga gacactggtg
     2101 cgtcacgcgg gatttgcctg tgtgcgccgg agccacctgg gcgaagggga tggacccgcg
     2161 ccagagccgg atgcggtgat tctgctcgat accatggggg agctggccgg cgtcttctcg
     2221 cttgccacag tagttttcct ggggggcagc ctggtgccgg tgggcgggca cgacattctg
     2281 cagcccctga tcgccggaaa gccggtcctg ctcggcccct acactcatcg ccagcgggag
     2341 atcgtggagg acgccctgga tgccggggtg gtgtgggtcg ttaccactcc tcaagagttg
     2401 gcacgccggt cgcttgaact gatgaaggac gccggtagtg gcgacggtta tgcggcgaac
     2461 gcagtcagct tcgtatcagg ccgacgcggt gcttcccagc gctgcgcgga ggaggcggcg
     2521 cgtcttttgg gagagatgca gccgttacgc acccatgggg ttgggaaggc ggtcggtaag
     2581 agttgagagg cgaggtcagc gctggatgcg agaacaggga gcgcctggcg gtgcgtttcc
     2641 cattttctgc ttctctcccg acgccttact tgctgcgcgt cgccgatggg gaggccacgg
     2701 gtccgctcgc gttcgggatc cgcgccgggc tgcgcgccct ctcctggctc tatctggccg
     2761 gcgtcggtgc ctcggtgctg gcgcatcgat cggggctact tcgctgcgag cgcgcgcctt
     2821 gcccggtgat cggcgttgga aatctcaccg ttgggggaac gggcaagagc acggcgaccg
     2881 ctgagttcgc ccgctggcta ctggaagccg gtctccgccc cgccgtgctc agccggggct
     2941 atggggtgca gggcagcccg ccggtgcgcg tcgtctcgga tggcaaaaac gtcttgctgg
     3001 gcccggagga ggggggcgac gagccggtga tgctggcgca tcacctggcc ggagttcccg
     3061 tgctcatcgg tcggcgcagg ccgcacaccg cgcgcgcggc cgtcgagcgc ttctctgccg
     3121 acgcctgcct gctggatgat ggcttccagg tatggaacct gcgtaaggat ctggaagtcg
     3181 tcctcctgga tagccgccgc cccttcgata acggctatgt gctccctcgc ggcatgttgc
     3241 gcgagccgcc gtcgcatctg ggtcgtgcgg atgccgtggt actgatggaa ccggatcggg
     3301 tggagcggga ggcgctggca cgtctgcgta agcaggtgga ggcgctcgcg cccggggcgc
     3361 ttgttgccga agccggccgt gttccagaac gcctctggga tcttgccaca ggccaggatt
     3421 accccctctc tgccctgaac ggccttccca tcgtggccct ttccgccatt ggcaacccgc
     3481 agggctttga ggacctgctg ggcagtttgg gcgcgcgaat ctatcctgcg cgcctgccgg
     3541 accaccatcc gtataccccc gatgatgtgg agtgggtcca gcgcctggcg aagcagaaag
     3601 gcgccgccgc catcattacg acggagaagg acgcggtgaa gctcgatgaa tgcggcgggg
     3661 cgcgaggccg ccggggcgag gcgggcgcct ggggcagggc gctggccacg gaggcttccc
     3721 aggagagcgt gatttcggcg gctcccgatg ggaaggcccc ggtgctggtc ttctccgtcc
     3781 gcttgcgctt taccgcgggc ggcgacgcgc tgcgcgagcg cgtgctggcg accgtgaggg
     3841 agaagcgttg aatacgcgtt tcggaaaacg gttgaagatg aaggag
//
LOCUS       DAOSCV010000376         3884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14791_length_3884_cov_3.407156, whole genome shotgun sequence.
ACCESSION   DAOSCV010000376 DAOSCV010000000
VERSION     DAOSCV010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14791_length_3884_cov_3.407156"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLY56_10000"
     CDS             <1..818
                     /locus_tag="PLY56_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ21856.1"
                     /translation="GELLFAALLSTLLFAYAGFILAVIWADVAWLAMPGAESGKRGIT
                     KVLTSPDVATEVLTALKLSLVTSFISSILAMTVAIPSAYALSRYRLPFRSLIDTVIDL
                     PIVIPPLIAGISLLIFFNQMGFGIQLEKWLRVVYSQRGIVVAQFFIASAFCIRATKAA
                     IDQVNPRFESVARSLGCGPVRAFWQITLPLAKTGLMAGWIMTWARAMGEFAPIMVFAG
                     ATPLYTAVLPVTAFLNLSVGNIETSVAVTVLMIVIASVTLLLFKKLGGQGYLW"
     gene            812..1912
                     /locus_tag="PLY56_10005"
     CDS             812..1912
                     /locus_tag="PLY56_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ21857.1"
                     /translation="MVDLRIEGVFIRLGEFDLRDISLTIEAGEYFCILGPTGAGKSVL
                     IECIAGLLRPDRGRIFLGDQDVTPLTPEQRNIGYVPQDYALFPHMSVRRNIGFGLRVR
                     RLPQKEIDKRVGQLAELLHIEPILEREPLHLSGGEKQRVALARALAVQPPVLLLDEPL
                     AAVDEQTRDRLCADLRDIQRRAGVTVIHISHNFEETLAVADRIGVMNAGRMVQVGTRR
                     QIFSAPATRFVAQFTRSENILPLAGSPRLLPDGRVRLPLDTGDEILAAPSEPLPNTPA
                     LAVVRPEAVRITPQNGEGTPKGLIPATLTRITERGHLLRLEARTDAGTSFVSLELRRD
                     ALLAGFQVGDRVHLHVPPEDVHLIPSEKDTGG"
     gene            complement(2012..>3884)
                     /locus_tag="PLY56_10010"
     CDS             complement(2012..>3884)
                     /locus_tag="PLY56_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21858.1"
                     /translation="GGVHSLAVALYWPTILTACLRFRGRDAVGVALLALLSLISIDTI
                     HHGSAVLLEGTEIALRAGGFLATALAGSALACRLRYEATQRALAAKDHESAGARLRRA
                     AFLDAVATIRREMQDPEAVIHAVLAPIRMETEATDVALLVADQDRGVLRVHAASGASA
                     ECLRGLKLPYDGSLAGECALWARPVVASSAHSGRRFQEAYEARHGAAHSSVLCVPLAR
                     GTSVVGVLALIDRRGLDAFTEEDLEFALSAAEHVADALRDAASRHEGHRQITEMAAFH
                     GFSRVLADGIRREEITSGLLDEAMMAVQADSGCVLLLDPDTGCLRPEVVRTPYRAYRL
                     PIVPLGEGAIGRVAVERLPALIAPLGREPLLLKLAGGRAASGALLLPLVTGNLLAGIV
                     ILVRATAFESHALAAVQGVVEQAAIAFKNEHLRETVRSGYLKTIRALAETVDAKDPYT
                     RQHSERVARYSRAIAEELGLPAGQIEAIEVGALLHDIGKIGISEAVLTKPGRLTEEEF
                     AQMKLHPVIGARIVEPLSFPADLLALVRHHHERFDGRGYPDGLGSTDLALSARIVAVA
                     DSFDAMGSDRAYRRALSLSNIIAELRRGAGSQFDPGIVDAFLSVLDRRGQELVPRLQ"
BASE COUNT          644 a         1418 c         1182 g          640 t
ORIGIN      
        1 tgggcgagct ccttttcgca gcgctcctct ccacgctcct cttcgcctat gcgggcttta
       61 tcctggcggt gatctgggcg gacgtggcgt ggctggccat gccaggcgcc gagtcgggca
      121 agcggggcat caccaaggtc ctcacttccc cggacgtggc gacggaagtc ctcacggcgc
      181 tgaagctgag cctggtcacc tccttcatta gctccatcct cgccatgacg gtggccatcc
      241 cctctgccta cgcgctctcg cgctaccgtc tccccttccg gtcgctcatc gatacggtca
      301 ttgaccttcc aatcgtgatt ccgccgctta tcgcgggcat cagcctcctc atcttcttca
      361 accagatggg cttcggcata cagctcgaga agtggctacg tgtggtctac tcgcagcgcg
      421 ggatcgtcgt cgcgcagttc ttcatcgcgt ccgccttctg cattcgcgcc accaaggccg
      481 ccatcgacca agtcaacccg cgcttcgagt ccgtggcgcg ctcgctcggg tgcggccctg
      541 tgcgcgcctt ctggcagatc accctccccc tggcaaagac cggcctcatg gccggatgga
      601 tcatgacctg ggcgcgcgcg atgggcgagt tcgccccgat catggtcttc gccggtgcca
      661 ccccgctcta tacggcagtc ctgccggtca ccgctttcct caacctctcc gtggggaaca
      721 tcgagacctc ggtcgccgtc accgtgctga tgatcgtcat cgcctcggtc actctgctcc
      781 tcttcaagaa gctgggggga caagggtacc tatggtagac ctgcgcatcg agggcgtctt
      841 catccgcctg ggcgagttcg acctgcgcga catctccctc acgatcgagg caggcgagta
      901 tttctgcatc ctggggccca cgggagcggg caagagcgtc ctcatcgagt gcatcgccgg
      961 cctgctgcgc cccgaccgcg ggaggatctt cctgggcgat caggatgtca ctcctctgac
     1021 gcccgagcaa cgcaacatcg ggtacgtgcc ccaggattac gccctcttcc cgcacatgtc
     1081 cgtgcgtcgg aatatcggct ttggcctgcg ggtgcgccgg ctgccccaga aggagattga
     1141 taagcgcgtc ggccagttgg cggagctact ccacatcgag ccgatcctgg agcgcgagcc
     1201 gctccatctc agcggaggag agaagcagcg cgtggccctc gcccgtgccc ttgccgttca
     1261 gccgccggtc ctactgctcg atgaacccct cgcggccgtc gatgagcaga cgcgcgaccg
     1321 cctctgcgcc gatcttcgcg atattcagcg ccgcgcgggc gtcaccgtga tccacatttc
     1381 ccacaatttc gaggagacgc tggccgtcgc cgaccgtatc ggcgtgatga atgccgggcg
     1441 gatggtccag gtgggcacgc gccgtcagat cttctccgcg cccgcgacac gcttcgtcgc
     1501 ccaattcacg cgctccgaga acattctccc cctggccggt tctccccgcc tcttgccgga
     1561 cggccgcgtg cgcctgccgc tcgacactgg ggacgagatt ctggcggctc catccgagcc
     1621 gctccccaat accccggcgc tggcggtggt ccgcccagag gcagtgcgta tcacacccca
     1681 gaatggcgag ggcacgccca agggcctgat cccggccacg ctcacgcgta tcaccgagcg
     1741 cgggcacctg ctccgcctgg aggcgcgcac tgatgccggc acatccttcg tctcgctgga
     1801 acttcgccgg gatgccctcc ttgcgggctt ccaggtgggc gaccgcgtcc atctccatgt
     1861 acctcccgag gatgtgcatc tgatcccctc ggagaaagac acaggagggt gaccacacct
     1921 ctcaacagcg caaggcacct ctctcgcgga acagatcgaa gcctctccgc gccgggaatc
     1981 ccctccccca ttcgcgggcc cgcagccgcc ctcactggag gcgcggcacc agctcctggc
     2041 cgcgccggtc gagcacgctc aggaacgcat cgactattcc ggggtcgaac tgcgagccgg
     2101 cgccgcggcg cagctccgcg atgatgttcg atagggaaag cgcgcgccgg taagcgcgat
     2161 ccgaccccat ggcatcaaag ctatcggcaa cggccacgat ccgcgcgctg agcgccagat
     2221 cggtgctgcc caggccatct ggatagccac gcccatcgaa gcgctcatgg tgatggcgca
     2281 ccagggccag aagatcggcg ggaaagctga gcggctccac aatcctcgcg ccgatcaccg
     2341 ggtgtagctt catctgcgcg aactcctcct ccgtgagccg gcccggcttg gtcaacaccg
     2401 cctcgctgat tccaatcttg ccgatgtcgt gaaggagtgc gcccacctcg atcgcctcga
     2461 tctggcccgc cgggaggccc agctcctcgg cgatggcacg ggagtagcga gccacgcgct
     2521 cgctgtgctg ccgcgtgtag ggatccttgg catcgacggt ctcggcgaga gcgcgaatcg
     2581 tcttcaggta gcccgagcgc accgtctcgc gcaggtgttc attcttgaag gcgatggccg
     2641 cctgctccac caccccctgc accgcggcca gcgcatggct ctcaaaagcc gtggcacgga
     2701 cgaggatgac gatgccggcg aggaggttgc ccgtgaccag gggcaacagc agcgctccgc
     2761 tcgcggctct tcccccggcg agcttcagca ggagaggctc ccggcccagg ggggcgatca
     2821 gagccgggag acgctccaca gcaacgcgac caatggcacc ctcgcccaac ggaacgatcg
     2881 gcaaccggta agcgcgatac ggagtgcgca ccacctccgg gcgcaagcac ccggtgtcgg
     2941 gatccaagag gaggacgcaa ccggagtcgg cctgcaccgc catcatcgcc tcgtccaaca
     3001 ggcccgacgt gatctcctcc cggcggatcc catcggccag cacgcgggag aatccatgaa
     3061 acgccgccat ctcggtaatc tgccggtggc cctcatgccg agaggccgca tcgcggaggg
     3121 catccgcgac atgctccgcg gcagagagcg caaactccag atcttcctcg gtgaaggcat
     3181 ccaggcctcg ccggtcgatc aatgcgagaa cccccaccac gctcgtcccc cgagccagcg
     3241 gtacgcacag caccgacgaa tgcgccgcgc cgtggcgcgc ctcgtacgcc tcctgaaacc
     3301 gccggccaga gtgggcggat gatgcgacga ccggacgcgc ccatagcgcg cactcgccgg
     3361 caagcgagcc atcgtatggc agcttcaggc cacgcaggca ttcggcactc gcgccgctcg
     3421 ccgcgtgtac ccggagcacc ccccgatcct ggtccgccac cagcagggcc acatcagtcg
     3481 cctctgtctc cattcgaatc ggcgccagca ccgcgtggat gaccgcctcc ggatcctgca
     3541 tctcgcgccg gatggtggct accgcgtcga gaaaggcggc tcgccgcagt ctcgctcccg
     3601 cgctctcgtg atccttcgcc gccaaagcac gctgcgtcgc ttcgtagcga agccggcacg
     3661 ccagggccga gccggccagc gccgtcgcca ggaagccacc ggcccgcagc gcaatctccg
     3721 ttccctcaag gagaaccgcg ctcccgtggt ggatcgtgtc aattgagatg agggagagca
     3781 gcgccagcaa ggccacaccc accgcgtcgc ggccacggaa acgcaggcag gcggtgagga
     3841 tcgtcggcca gtagagcgcc accgccaggc tgtgcactcc gccc
//
LOCUS       DAOSCV010000377         3883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14794_length_3883_cov_3.612330, whole genome shotgun sequence.
ACCESSION   DAOSCV010000377 DAOSCV010000000
VERSION     DAOSCV010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14794_length_3883_cov_3.612330"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLY56_10015"
     CDS             <1..207
                     /locus_tag="PLY56_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21859.1"
                     /translation="GRVLKLAYCPSPSCYPLPAFADRTLNGFPLTPRHSEGCNVLYCD
                     GHVKWVRQETLYSNPNDIFGHSSR"
     gene            560..1849
                     /locus_tag="PLY56_10020"
     CDS             560..1849
                     /locus_tag="PLY56_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ21860.1"
                     /translation="MIREPLVPLFDLVLCVSDAVDLVSPNVAQHHKQVAYLAQAIARE
                     MGVPHDTRHEIVLAGMLHDIGALSLKERLELLQFETRDPHRHAEIASRLLFSFPRLRK
                     AGELVRFHHLPWDEGRGEEFHGLPVPLESHIIHLADRVAVSIDRRQGILVQAPNICKR
                     IAAQRGRLFSPAVVDAFLAAAERECFWLDATSPSLTPALRETVAMDSIELGPDEILAA
                     TNLFRQIIDFRSRFTARHSAGVAAVAEALAALFGFSARELRLMRVAGYLHDLGKLAVP
                     AEILEKPGRLTDEEYSIMRAHTFHTYYTYRTLRSVHGMAVVSAWAAFHHERLDGSGYP
                     FHLKAADLPLGSRIMAVADTFTALTEDRPYRNGMDVDRAFSILMEMAEKRALDREIVE
                     LARGHLGKINDARATAQADATEEYRRLFAELDTAPSP"
     gene            complement(1855..3579)
                     /locus_tag="PLY56_10025"
     CDS             complement(1855..3579)
                     /locus_tag="PLY56_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ21861.1"
                     /translation="MKRITHRADFCVVGGGLAGMCAAIAAARHGAKVVLMHDRPVLGG
                     NASSEIRMWICGARGPNNRETGIVEEIQLENLYRNTSVSYAIWDAILYEKVRFQPNLT
                     LLLNCSCTDLGMDGSRIRWVRGWQGTAETWHTVEATLFADCSGDSILAPLSGAEFRVG
                     REAASEFGEQMAPEAADRKTMGMSCLIQARETESPQPFIPPAWANKYLSDDDLPHRGH
                     GIRTSNFWWMELGGDFDSIHDTEMLRDELLKVAYGVWDHIKNHGDHGAENWILDWVGF
                     LPGKRESRRYVGDHILTQSDVNAEGRFDDVVAYGGWPFDDHHPAGIRHPGFPNEAIPA
                     PSPFGIPYRALYSRNIENLLFAGRNISTTHVAMSTTRVMATCATLGQAVGTAAALAVD
                     AGLTPRGVYHECIRELQQRLMEDDAYLPWNTREVPELTRTARLTASEGDPEPLRNGLD
                     RPIGDADNGWTGSLGSWVEYAFDGPREVKECRFVFDSDLNRRRLNMPSCFPRDLEPHR
                     VPETLVRTFRIEVRNESGEWTLATRVEESHQRLVRVPVNVTTSAIRFIPEATWGAPQA
                     HLFAWDVR"
     gene            complement(3693..>3883)
                     /locus_tag="PLY56_10030"
     CDS             complement(3693..>3883)
                     /locus_tag="PLY56_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21862.1"
                     /translation="VWLSRTEAGTWRLVSSVDVRLNGSVRYRKPGGAWTVARDGLVPA
                     TGMEASLEVEWKPAGAAR"
BASE COUNT          665 a         1353 c         1201 g          664 t
ORIGIN      
        1 ggcagggtct tgaagctcgc ctactgtcct agccctagct gctaccccct gcccgccttc
       61 gcggatcgca cgctcaacgg tttcccgttg acaccccgac acagcgaggg gtgcaacgtc
      121 ctctactgcg atggccacgt gaagtgggtg cggcaagaga cgctctacag caaccccaac
      181 gacattttcg ggcactcctc tcgctaggca ggtggcgcgc aaggccgtcg ctttcgccca
      241 accacggtag cgcggcgtcc gatgtcgcgc gccgagagcg gagggtgctg agaccttccg
      301 ccgcttgcat actctagatc gcgcggcagt caaggaacag gccggggtca atcgtccggc
      361 ctgttcccgc ccttaacccg ggccgggccc cctgccacgc gcctgccagg atgacagcgt
      421 cgggcccaca agagttcaca taccttacag aattgcctct ctgccggcgg ccgtatcccg
      481 agcccctatt tcagactcgg cagttgtgca tgatccgaac gccgatgtac ccgcggcggc
      541 ctttgatgga gatggtggcg tgattcgaga acccctagtc ccgcttttcg acctcgtcct
      601 ctgcgtgtcg gacgccgttg acctggtgag cccgaacgtt gcccagcacc acaagcaagt
      661 cgcctacctc gcgcaggcga tcgcccgcga aatgggcgtc ccgcacgaca cccggcacga
      721 gatcgtactc gccggcatgt tgcacgacat cggcgcgctc tcgcttaagg agcggctgga
      781 gctcctccag ttcgaaaccc gggatcccca ccggcatgcc gagatcgcca gccggttgct
      841 cttctcgttt ccacgtctgc gcaaggcggg tgagctggtg cggttccacc atctcccctg
      901 ggacgagggg cgcggtgagg agttccatgg actccctgtg cccctggaga gccacatcat
      961 ccacctcgcc gaccgcgtcg ccgtctccat cgaccggagg caaggcattc tggtccaggc
     1021 gcccaacatc tgcaagcgga tcgcggctca gagggggagg ctcttctcgc ctgcggtcgt
     1081 cgatgcattc ctcgcagcag cggagcgcga gtgcttctgg cttgatgcca catccccttc
     1141 tctcaccccc gcgctccgag agaccgtggc catggatagt atcgagctgg gccccgacga
     1201 gattctcgcg gcgaccaacc ttttccgaca gatcatcgac ttccgcagtc gcttcaccgc
     1261 ccgccactcc gccggggtcg ccgccgtggc cgaagccctc gccgcgctct tcggcttttc
     1321 cgcccgcgaa ctccggctga tgcgcgtcgc cggctacctg cacgacctgg ggaagctggc
     1381 ggtacccgcg gagatcctcg agaagccggg ccggctcacc gacgaggagt atagcatcat
     1441 gcgggcacac acgttccata cctactacac ctaccggacg ctacggagcg tgcatggcat
     1501 ggcggtcgtg agcgcctggg ccgccttcca tcacgagcgc ctcgacggta gcggctaccc
     1561 cttccacctg aaggccgccg atttgccgct gggctcgcgg atcatggcgg tcgcggacac
     1621 gttcaccgca ctcacggaag accggcccta tcgcaatggg atggatgtcg accgtgcgtt
     1681 ctccatcctc atggagatgg cagagaagcg ggcgctcgac cgggagatcg tggagctggc
     1741 gcgcgggcac ctgggcaaga tcaacgatgc ccgcgcgaca gcacaagcag atgcaacgga
     1801 ggagtaccgg cgcctcttcg cggagctcga cacggcgccg tcaccatagc ggagctaccg
     1861 cacgtcccag gcgaagaggt gcgcctgcgg ggcaccccag gttgcctccg ggatgaaacg
     1921 gatggcggaa gtggtgacgt tcacgggcac gcgcaccagg cgctgatggc tttcctccac
     1981 ccgcgtggcc agcgtccact cgccactctc gtttcgcacc tcgatgcgga aggtgcgaac
     2041 gagcgtctcg ggcacccgat ggggctccag gtcgcgcggg aagcaggagg gcatgttcag
     2101 acggcgccgg ttgaggtcgc tatcgaagac gaagcggcac tccttcactt ctcgtggacc
     2161 atcgaaagcg tactccaccc acgacccgag gctccccgtc cagccgttgt ctgcgtcgcc
     2221 gatggggcga tccaggccgt tgcgcagcgg ctcggggtct ccttcagaag ccgtgagccg
     2281 ggcggttcgg gtgagctcgg gcacctcgcg ggtgttccag gggaggtagg catcatcctc
     2341 catcagcctc tgctggagct cgcggatgca ctcgtggtag acgccgcgcg gagtgagccc
     2401 cgcatccacc gcgagggccg cggccgtgcc caccgcctgg ccaagcgtgg cacaggtggc
     2461 catgacgcgc gtcgtgctca tcgccacgtg cgtggtgctg atgttgcgcc cggcaaagag
     2521 gaggttctct atgttgcgcg agtagagcgc ccggtagggg ataccgaaag gcgagggcgc
     2581 cgggatggct tcattcggga atccagggtg gcgtatcccg gcggggtggt gatcatcgaa
     2641 cggccagcca ccgtaggcca ccacgtcatc aaatcgaccc tcggcattca cgtcgctctg
     2701 cgtcaggatg tggtcaccca cgtaacgccg gctctcacgc ttgccgggga ggaagcccac
     2761 ccaatcgagg atccagttct ccgcgccgtg atcgccgtgg ttcttgatat ggtcccacac
     2821 gccgtaggcg accttcagga gctcgtcgcg gagcatttcg gtgtcgtgaa tcgagtcgaa
     2881 atcgccgccg agctccatcc accagaagtt cgaggtgcgg atcccgtggc cgcggtgcgg
     2941 caagtcgtca tcgctgaggt acttgttcgc ccatgcgggg gggataaacg gctgcggcga
     3001 ttcggtctcg cgcgcctgga tcaggcagct catccccatc gtcttgcggt cggccgcctc
     3061 tggggccatc tgctcgccaa actccgaagc agcctcgcgg ccaacgcgga actcggcgcc
     3121 cgagagcggg gcgaggatgc tatccccgga gcaatcggcg aaaagtgtgg cctccaccgt
     3181 gtgccacgtc tcggcggtgc cctgccagcc acgcacccag cggatccgcg agccgtccat
     3241 tcccaggtcg gtgcacgagc agttcaggag aagcgtcagg ttcggctgga agcgcacctt
     3301 ctcatacagg atagcatccc agatcgcgta gctgaccgag gtgttccgat agagattctc
     3361 gagctggatc tcctccacaa tgccggtctc ccggttgttc ggcccgcggg cgccacagat
     3421 ccacatgcga atctcggaag aggcattccc tcccagcacc gggcggtcgt gcatcaagac
     3481 caccttcgcg ccatggcgcg ctgcggcaat cgccgcgcac atgccggcca acccaccccc
     3541 taccacgcaa aaatcagctc gatgagtgat cctcttcatc agaaaaacct ccacgtggcg
     3601 gcaccatcag ccgctcctca cctccaattg gcgatccccg accgcttctc ctgcgctgac
     3661 caccagccta tcgagagctc ggcgtgagcc cactacctcg cggcccccgc gggcttccac
     3721 tccacctcca ggctggcttc catgccggtg gcaggaacaa gaccatcccg ggccacagtc
     3781 cacgccccgc ccggcttccg gtagcgcacg ctcccgttca ggcgcacatc gacgctcgaa
     3841 acgagccgcc acgtgccagc ctcggtgcgc gagagccaga ccg
//
LOCUS       DAOSCV010000378         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14818_length_3878_cov_3.424536, whole genome shotgun sequence.
ACCESSION   DAOSCV010000378 DAOSCV010000000
VERSION     DAOSCV010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14818_length_3878_cov_3.424536"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLY56_10035"
     CDS             complement(<1..765)
                     /locus_tag="PLY56_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ21863.1"
                     /translation="MNKKTVRDVDVAGKRVLVRVDFNVPQDESGQITDDTRIRAALPT
                     IQYLVDHGARTILVSHLGRPKGADDKLRLDPVAARLQELLGRPVQKANDSIGPEVQEQ
                     AAKLQDGDVLLLENVRFYKEEEKNDPEFARALASLADLYVNDAFGTAHRAHASTEGVA
                     HLLPGVAGFLMEKEIDYLGRVLESPERPFVAILGGAKVKDKIGVIENLLPKVDALLIG
                     GAMAFTFVKAQGGEIGDTLLDEASLGVATEMLAKAKE"
     gene            complement(1088..1969)
                     /gene="ftsY"
                     /locus_tag="PLY56_10040"
     CDS             complement(1088..1969)
                     /gene="ftsY"
                     /locus_tag="PLY56_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOJ21864.1"
                     /translation="MFRGLFRKIDDLLASRRRSIDDELFDDLEELLVCADVSIHTAVR
                     IVGELREAVRRDRIRDADEVREQLKRELVTLLMEGHRPLNQAPSPPAVYTFVGVNGVG
                     KTTSIAKTAHMLKRQRKRVLLAAADTFRAAAIDQLEIWGQRVGVDVIRQKEGSDPAAV
                     VYDAIQAARARHVDVVLVDTAGRLHTKANLMEELRKIHRVIERELGRPPDETLLVLDA
                     TTGQNAVSQARTFKDAVAVTGIVLSKLDGTAKGGNILTIQDELKIPIILIGTGEQLED
                     MEPFDPKAFVDALFAEE"
     gene            complement(1969..>3878)
                     /locus_tag="PLY56_10045"
     CDS             complement(1969..>3878)
                     /locus_tag="PLY56_10045"
                     /inference="COORDINATES: protein motif:HMM:NF019077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HOJ21865.1"
                     /translation="SLLAAKDALYAGTGDRGVVYRIAGESATPVLKTGELQVLALAQD
                     AAGNLYAGTAPRGSLYRLTPEGKSELFWRSPEQYILSLAVDAQGNLYAGTSPHGGVYR
                     ITPDGKGELLARIPDPYVVSLAVSGANLYASGGMGAVVYRITDDRKVSTLFDPGQGTT
                     ASLITVDPTGQLYVGTSPRGALYRVEPDGRATLLHEKSSAGINAIAADGRGALWAAAG
                     DTIYRVSGSPERPVVEKIQPPVAPRFLALATDEAGRVAVGSATGDVYRLAAGSPRRGV
                     LESAVRDAGSVARWGRLTWQARVPAGTRVVLQTRTGNSGEPDATWSPWSAEQTRSGAT
                     VASPPGRYFQYRAILTSEDPSVQPVLSEVAVVYLAHNQKPKLTVQAPKSGDVWSRKQT
                     IRWSGTDPDKDTLLYDVYYSGDEGASWTRLNRIVKEAPAGTPENGPAGNGAAPSAKAP
                     AAPPAAGGKGGATKAPAAAMSPAPANSSNAPAPGAPKRQDPSLKETSFTWDTTEVPDG
                     RYLVKIVASDRLSNPADPASVEEIIGPILISNTPPKLALQGDPAADEAGCIHLAGTAT
                     AGVPLAGVDYRIDAEEWASAEAEDGIFDSETERFRVKTTALDAGEHTIEIRAVDAAGN
                     HVVEKRKVTIP"
BASE COUNT          605 a         1347 c         1253 g          673 t
ORIGIN      
        1 ctccttcgcc ttggcgagca tctcggtagc cacgcccagg ctggcctcat cgaggagggt
       61 gtcgccgatc tcgccgccct gcgccttgac gaaggtgaac gccatcgccc cgccgatgag
      121 gagggcatcc accttgggca ggaggttctc gatgacgccg atcttgtctt tcaccttggc
      181 gccgcccaga atggcgacga aggggcgctc cgggctctcc aacacgcgcc cgaggtaatc
      241 gatctccttc tccatcagga agccggccac acccggcagg aggtgggcca cgccctcggt
      301 ggacgcatgc gcgcggtgcg cggtgccaaa agcatcattc acgtagagat cggcgagcga
      361 ggcaagggcg cgcgcgaact ccggatcgtt cttctcctcc tccttataga agcggacgtt
      421 ctccaggagg agcacatcgc catcctgcag cttcgccgcc tgctcctgga cttcgggccc
      481 gatggaatcg ttcgccttct gcacgggccg gccgagcagc tcctggaggc gcgcggcgac
      541 cggatcgagg cgcagcttgt catcggcgcc ctttggccgc cccaagtggg agacgaggat
      601 ggtgcgggcg ccgtggtcca ccaggtactg gatagtcggg agcgcggcgc ggatgcgcgt
      661 gtcgtcggtg atctggccgc tctcatcctg aggcacgttg aagtccacgc gtaccagcac
      721 gcgcttcccg gctacatcca cgtccctcac cgtcttcttg ttcatcagac cctcctcacg
      781 ctaggcatca caagagaata acgcaaccat cggtgcgggg cctgcgccgt tggggtgccc
      841 ccaccccgcg ggcggcaatc aaccgcatgg agaccacgcg ccgacactcg tcattataac
      901 gggcaggcgg aggcgcgtca agggagcttc caccggcgcc gggtggccgc gcggtgactt
      961 atcgccgcgc gctccgggtg gggcggttgt tcacgcccac gcgttgctcc cccatgacgg
     1021 gcaatgaaaa accccggggg gcggtgctcc cccggggctg gcatagtagc cgagacctac
     1081 ctagcgctca ctcctcggcg aagagtgcgt ccacgaaggc cttcggatcg aacggctcca
     1141 tgtcttcgag ttgctcgccg gtgccgatga ggatgatcgg gatcttcagc tcgtcctgaa
     1201 tcgtcaggat gttgcctccc ttggccgtgc catccagctt ggacagcacg atccccgtca
     1261 cggcaacggc atccttgaag gtacgcgcct gcgagacggc gttctggccg gtggtcgcgt
     1321 ccaggacaag cagcgtctca tcaggcggcc ggcctagctc gcgctcgatc acgcgatgga
     1381 tcttgcgcag ctcctccatc aggttcgcct tggtgtgcag gcgccccgcc gtatccacca
     1441 ggacgacatc gacgtgccgg gcgcgtgccg cctggatcgc gtcgtagacc acggcggcgg
     1501 gatcggatcc ttccttctgg cggatcacat ccacgccgac gcgctgcccc caaatctcca
     1561 gctggtcgat ggcagccgcg cggaaggtat ccgcggcggc gagaagaacg cgcttccgct
     1621 gccgcttcag catgtgcgcc gtcttggcaa tgctggtcgt cttcccgaca ccgttcacgc
     1681 caacgaaggt gtagacggcg ggaggtgacg gcgcctggtt gaggggccgg tggccttcca
     1741 tcaggagggt caccagctcc cgcttgagct gctctcgcac ctcgtcggcg tcgcgaatcc
     1801 ggtcgcgacg cacggcctcg cgcaactcgc cgacgatgcg gaccgcggtg tggatgctca
     1861 catccgcgca caccaacagt tcctctaggt cgtcgaacaa ctcgtcatcg atgctgcgtc
     1921 gccgagacgc gagcaggtca tcgatcttcc ggaaaagccc tcggaacatc agggtattgt
     1981 tacctttcgc ttctctacca cgtggttacc ggcggcgtcc accgcgcgga tctcgatggt
     2041 gtgctcgccc gcgtccagcg cggtcgtctt cacccggaag cgctcggttt ccgagtcgaa
     2101 gataccatcc tcggcttcag ccgacgccca ttcctccgca tcgatgcggt aatcgacgcc
     2161 tgccaggggc acgccggcgg tcgcggtgcc ggcgaggtgg atgcaaccag cctcatccgc
     2221 tgcaggatcg ccctggagcg cgagcttcgg cggcgtgttc gagatgagga tcggaccgat
     2281 gatctcctcg acactcgcgg gatccgccgg gttgctcagg cggtcgctgg ccacgatctt
     2341 caccaggtag cggccatcgg gcacttccgt ggtgtcccag gtgaagctcg tctccttcaa
     2401 ggagggatcc tgacgcttgg gagcaccggg cgcgggggca ttcgacgaat tcgccggtgc
     2461 ggggctcatc gccgccgcag gcgccttcgt ggcccctccc ttgccaccgg cggcaggcgg
     2521 ggccgccggc gccttggcgc ttggtgccgc cccgttcccg gcggggccgt tctcaggcgt
     2581 gccggcaggc gcctccttca cgatgcggtt cagccgcgtc caggacgccc cctcgtcccc
     2641 cgagtagtaa acgtcgtaga ggagcgtatc cttgtccggg tccgtcccgc tccaacggat
     2701 cgtctgcttc ctggaccata catcgccgct cttgggcgcc tgcaccgtca gcttcggctt
     2761 ctggttgtgc gccagataga cgaccgccac ctcggaaagc accggctgca cggacggatc
     2821 ctcgctcgtg aggatcgccc ggtactggaa gtagcgtccg ggcgggctgg ccaccgtcgc
     2881 gccggagcgc gtctgctctg cggaccaggg gctccaggtg gcgtctggct cgcccgagtt
     2941 cccggtgcgt gtctgcagca ccacgcgcgt tccagccggc acgcgcgcct gccaagtgag
     3001 ccggccccac cgagccaccg agcccgcgtc acgcacggca gactcgagca cgccacgccg
     3061 cggcgaaccg gccgccagcc ggtagacatc gccggtggcg cttcccacgg cgacccggcc
     3121 ggcctcatcg gtcgcaagcg ccaggaagcg gggagccacc gggggctgga tcttctccac
     3181 cactgggcgc tcgggagagc cgctcacacg gtagatggta tcgcccgcag cagcccacag
     3241 agcgccccgg ccatccgcag ctatggcatt gatcccggca gacgacttct cgtgcaacag
     3301 cgtcgcgcgc ccgtcaggct caacgcggta gagcgcgcct cgtggcgagg tgcccacata
     3361 gagctgtccc gtgggatcta ctgtaatcag cgacgcggtg gtgccctgcc caggatcgaa
     3421 cagcgtgctc accttcctat cgtcggtgat tcggtagacc acagcgccca ttccgccgct
     3481 ggcgtacagg ttggcccctg ataccgcgag cgagaccacg tacggatcgg gaatgcgcgc
     3541 caggagttcg cccttgccat cgggagtgat gcggtagacc ccaccgtgcg gcgacgtgcc
     3601 ggcatacaga ttgccttgcg cgtccaccgc aagcgagagg atgtactgct cagggctgcg
     3661 ccagaagagc tcgctcttgc cctccggcgt caggcgatag agtgagccgc gcggcgcggt
     3721 tcccgcgtag agattgcctg ccgcatcctg cgccagggca agcacctgca gctcgcctgt
     3781 cttcagtacg ggggttgcgc tctcacccgc gatccgatag acgacgcccc gatctcccgt
     3841 tccggcatag agggcatcct ttgccgctag gagagacc
//
LOCUS       DAOSCV010000379         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14821_length_3878_cov_2.556108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000379 DAOSCV010000000
VERSION     DAOSCV010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14821_length_3878_cov_2.556108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1523)
                     /locus_tag="PLY56_10050"
     CDS             complement(246..1523)
                     /locus_tag="PLY56_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ21866.1"
                     /translation="MDLLAVAGRAEALADETIALAQALVRANTVNPYSGDPTWGNEAN
                     GQAILEPVLRDLGARIERFDCPPDVYQRAGVLGPKNREFAGRPNLQAEMVFSESGPRV
                     VLQAHMDTVGISDMTIEPFSGEVRDGRLYGRGSSDCKGGIAATVAVLRVLHELRGQLR
                     GSVVFQSVVDEECNGGGAGAIACCLRGLKADLGIVTDGTGPFVTRGYSGVLTVEITVP
                     GVSGHAARPDGVSAIEKALVVKEALDAFKRDRESTRKHAAVNLGILRAGIHPAVIPGE
                     AFLALNMSYPIADARAAEEAGAGFGGAPIRADFERRIRERESQDPFLATHPSRIEWVK
                     DLIPFETPADHPLVTGLAETHRAVLGEEPSMEVNPAWSDGCYLPALCGIPTVAYGAGV
                     PGKAHSADEYGEVERIINCSRVLSAYLYRVLAA"
     gene            complement(1527..2576)
                     /locus_tag="PLY56_10055"
     CDS             complement(1527..2576)
                     /locus_tag="PLY56_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21867.1"
                     /translation="MSDDTIRIGIVGAGANTRARHIPLLQRISGVRLMAVCNRRPEST
                     RRVAQEFGIPLTFERWEELVSSPEVDAVVIGTWPYLHCAVTVAALEAGKHVLCEARMA
                     MNAAEAHRMLEASRAHPDLVAQVVPAPMSLRVDPTVRRLLDEGYLGELLVANVRGVTG
                     AFVDPEVPLHWRQDIRLSGLNALTMGIWHEILMRWVGNASRVLARRRVFVPERTDPET
                     GLRRVVCVPDHIDIIADLECGAQATYQFSSVLGLGGGSGIALYGTEGTLVFDGDNDRL
                     LGGRRGDQELAEIPIAPEEEGRWRVEEEFIGAIRGEVPVRLTTFEDGVAYMEFTEAVA
                     RSAEQGRAIRLPLEE"
     gene            complement(2701..3438)
                     /locus_tag="PLY56_10060"
     CDS             complement(2701..3438)
                     /locus_tag="PLY56_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ21868.1"
                     /translation="MKITIIGQSTCIPDQERETACSLIDGKHLVDTGWCSAQAMRPYG
                     FDPLALESILLTHLHQDHYLGLPALLFHHGLRGRGERPPLQIAGPREYLESVVGAALE
                     FLQVERFPEITPNLTLLPLSSGERFTLDDLTVETFGARHVSGHNRHEPTLAYRFTNAA
                     GKSVVFSGDTHPHPPLAEFARGAAVLIHDGAHTPAREAAAIARDAGVGRLLLTHYREE
                     DGPTLLQEAREVFSDTDLAEEGMTLLI"
     gene            complement(3440..>3878)
                     /locus_tag="PLY56_10065"
     CDS             complement(3440..>3878)
                     /locus_tag="PLY56_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21869.1"
                     /translation="LWTVRRSYPLIGCTLPERQTYDLLCGLAALRREAQVHSIALYGK
                     GWTAVLALYAALFDPEVSEVILEAPPETHEDPKAPEFLNILSVGDLPENLALMHPRPV
                     TFVGAMPPAYERVRQVYIDRETPTRFRVLESMAEWRPAEEGPR"
BASE COUNT          630 a         1393 c         1270 g          585 t
ORIGIN      
        1 gttaccaccc cttcaccgcc gagatcgacc acttcgtcga gtgcgtcctg gatggccgcc
       61 agcccacccc gaacatcgag gatgcctgca agacgcagga ggtgtgcctg gccgcggagc
      121 tttcggcgca acgcgacggg gagacggtgc ggatcgaggc atagcccgcc gcccgcatgc
      181 ggaaggcgtg attggtccgg ggcccgcgca agccgcgagc cccggcaccg gctggtgccg
      241 cgcggttacg ccgccagcac ccggtacagg taggccgaaa gaacgcgcga gcagttgatg
      301 atccgctcca cctcgccata ctcatccgcg gagtgcgcct tgccgggcac gccggcgcca
      361 taggccaccg tcggaatccc gcaaagcgcc ggcaggtagc agccgtcgct ccaggcgggg
      421 ttcacctcca tggatggctc ctcccccagc accgcgcggt gcgtctcggc caggccggtc
      481 accagcgggt gatccgccgg cgtctcgaat ggaatcagat ccttcaccca ctcgatccgc
      541 gagggatgcg tggcgaggaa gggatcctgg ctctcgcgct cgcggatgcg acgctcgaaa
      601 tcggcgcgaa tcggcgcgcc gccaaagccg gcccccgctt cctccgccgc gcgagcgtcg
      661 gcgatcggat agctcatgtt gagcgccagg aacgcctcgc ccgggatcac cgcggggtgg
      721 atgccagcac gcaggatccc caggttcacc gcggcgtgct tgcgggtgct ctcgcggtcg
      781 cgcttgaagg cgtccagcgc ttccttcacc accagcgcct tctctatcgc gctcacgccg
      841 tccgggcgcg cggcatgccc gctgaccccg gggacggtga tctcgacggt gagcacgccg
      901 gagtagccgc gagtgacaaa aggtccggtg ccatccgtga cgatccccag gtcggccttc
      961 aggccgcgca ggcagcatgc gatcgcgccg gcacccccgc cattgcactc ctcatccacc
     1021 acgctctgga atacaacaga gccgcgcagc tggccccgca gctcgtgcag cacgcgcagg
     1081 acggccaccg tcgcggcgat cccgcccttg caatcgctgc tgccccggcc ataaagccgg
     1141 ccatcgcgta cctccccgga gaagggctcg atcgtcatat ccgagatgcc cacggtgtcc
     1201 atgtgcgcct ggaggaccac gcgcggcccg ctctcgctga aaaccatctc cgcctggagg
     1261 ttgggccggc cggcgaactc tcggttcttt ggcccgagca ccccggcgcg ctggtagaca
     1321 tcgggcgggc aatcgaagcg ctcgatccgc gcgcctaggt cgcgcagcac gggctccaga
     1381 atcgcctgac cgttcgcctc gttcccccag gtggggtcgc ccgagtaggg attcaccgtg
     1441 ttggcgcgca cgagtgcctg cgcgagcgcg atcgtctcat cggccagcgc ctccgcgcgg
     1501 ccagcaaccg caagtagatc caccgttcac tcctccagcg gcaggcggat ggcccggccc
     1561 tgctccgcgc tgcgcgcaac cgcctcggta aactccatgt aggccacgcc atcctcgaaa
     1621 gtggtcaggc gcaccggcac ttcgccgcga atcgcgccga tgaactcttc ttccacacgc
     1681 caccgcccct cctcttcggg ggcgatagga atctcggcga gctcctggtc gccgcggcgg
     1741 ccgcccagga ggcggtcgtt gtcgccgtcg aacaccaggg tgccctcggt gccatagagc
     1801 gcgatgccgg agccgccgcc caggccgaga acggagctaa actggtaggt agcctgagca
     1861 ccgcactcca ggtcggcgat gatgtcgatg tggtcgggca cgcagaccac ccggcgcagg
     1921 ccggtctccg gatcggtgcg ctccggcacg aagacgcggc gccgggctag gacgcgcgaa
     1981 gcattcccca cccagcgcat caggatctcg tgccaaatcc ccatcgtgag cgcgttcagc
     2041 ccgctgaggc ggatatcctg acgccagtgc agcgggactt ccggatcgac aaacgcgcca
     2101 gtcacgcccc ggacgttcgc gacgagcaac tcgcccaggt agccctcgtc caaaagccgg
     2161 cgcaccgtcg gatccacgcg caggctcatc ggcgcgggca ccacctgcgc cacgaggtcc
     2221 ggatgggcgc gcgacgcctc cagcatgcgg tgcgcctccg cggcgttcat cgccatgcgc
     2281 gcctcgcata ggacgtgctt ccccgcctcc agcgccgcca cggtcaccgc gcagtgaaga
     2341 tagggccagg tgccgatgac gaccgcatcc acctccggcg agctgacgag ctcttcccaa
     2401 cgctcgaagg tgagaggaat cccgaactcc tgggcaaccc gccgggtcga ttccggccgg
     2461 cggttgcaca cggccatcag gcgcactccc gagatgcgct gcagcagggg aatatgccgg
     2521 gcgcgcgtgt tcgctcccgc gcccacgatg ccgatgcgga tcgtgtcatc gctcatcttc
     2581 cactccctcc cgcagtgttc cgatggaagc agcgacgcgt agaccagcgc ggcgaggagg
     2641 atgggcccgc gctcctccgc gacctgcccc tctcccggtt ctctatgagc acaccgtgcc
     2701 tcagatcagc agcgtcattc cttcctcggc cagatcggta tcactgaaca cctcgcgcgc
     2761 ctcctggagg agcgtcgggc catcctcctc gcgatagtgg gtgagcagga gccggccgac
     2821 tccggcatca cgcgcgatgg cggccgcctc gcgcgcgggc gtgtgcgccc cgtcatggat
     2881 cagcaccgcg gccccccgcg cgaactccgc gagcggcgga tgtgggtgcg tatcgcctga
     2941 aaagacgacg ctcttccccg ccgcgttcgt gaagcgatag gcgagcgtcg gctcgtgccg
     3001 gttgtgcccg gagacatgcc gcgcgccgaa cgtctccacc gtcaggtcat ccagcgtgaa
     3061 gcgttccccc gacgagagtg gcaacagcgt gaggttcggc gtgatctccg ggaagcgctc
     3121 gacctggaga aactccagtg ccgcgccgac caccgactcc aggtactctc tcgggccggc
     3181 aatctgcagg ggcgggcgct ccccgcgccc gcgcaggccg tgatggaaga ggagcgcagg
     3241 caggccgaga tagtggtcct ggtgcaggtg cgtcagcagg atgctctcca gcgccagcgg
     3301 gtcgaagcca tacgggcgca tcgcctgggc gctgcaccac ccggtatcca ccaggtgctt
     3361 gccgtcgatc agcgagcagg cggtctcgcg ctcttgatcg ggaatgcaag tcgactggcc
     3421 gatgatcgtg atcttcatct cagcgcggtc cctcttccgc cggccgccac tcggccatgc
     3481 tttcgagaac gcgaaagcgc gttggggtct cacgatcgat gtagacctgg cgcacgcgct
     3541 cgtaggccgg gggcattgcg cccacgaagg tcaccgggcg cgggtgcatc agcgccagat
     3601 tctccggaag gtcaccgacg ctcaggatgt tgaggaactc gggcgccttc ggatcctcgt
     3661 gcgtctcggg cggggcttcg aggatcactt cgctcacttc cgggtcgaag agcgccgcgt
     3721 agagggccag gaccgcggtc catcccttgc cgtagagcgc gatggagtga acctgggctt
     3781 cccggcgcag cgccgccaag ccgcagagga gatcatacgt ctggcgctct gggagggtac
     3841 acccgatgag gggataggag cggcgcaccg tccacagg
//
LOCUS       DAOSCV010000380         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14909_length_3861_cov_3.277194, whole genome shotgun sequence.
ACCESSION   DAOSCV010000380 DAOSCV010000000
VERSION     DAOSCV010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14909_length_3861_cov_3.277194"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLY56_10070"
     CDS             <1..274
                     /locus_tag="PLY56_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ21870.1"
                     /translation="AHIGNCVMRDSSHPAYGDEHPRFGVEGGENGVAELADFMQELLR
                     CGYLKAGEPRIVSFEVKPIAAWGETSQVVVANAKRALQKAWAQVQL"
     gene            372..1223
                     /locus_tag="PLY56_10075"
     CDS             372..1223
                     /locus_tag="PLY56_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21871.1"
                     /translation="MADRKRQTTIRTVRELLFTQPLARLYSSPCVGRAGQTSDTQEPF
                     AEVIAEELLRIQVAQLLGSVGIPPQRAMPYRQGHDGTVSTPTTPRSATSERRLAIALY
                     NYSRDHGGDCFGLLGQPIDYEVPLQAQRGAGIGEIDLLTQVKEESPGIENARGVYVVE
                     LKRKREKSPESLLRSVLQIATYYQQLDREKLLRDYGLYGDPSGYGTHEIRKAVLVFRG
                     SAQEREIQAIKKGSLPNLRRLIEDLGVHLFVIDADDTVVDQGTGPYRPVLQGGGAGVR
                     SVKRVLL"
     gene            complement(1236..2240)
                     /locus_tag="PLY56_10080"
     CDS             complement(1236..2240)
                     /locus_tag="PLY56_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21872.1"
                     /translation="MLTAEMVKKEAKALGADLVGIASMDRYEGAPLQMDPRQIMPEAR
                     SLIVMAFRVMRGSLRGIEEGTFFSNYSAMGYGGLTYLYMPLVVINLCRFIEDHGKEAI
                     PIGHQSDWRAIDGNGFLREGYSRPVEPGRAAPDVMIHLRIAGFLAGLGEIGYSKVFLT
                     PQFGPRQRLGVVLTEAELEPDPIYNGPKLCNRCMACVRECPGKAISATKTVKVTLAGH
                     EVEWGELDTAACDIAFRGGQVADPDHDSPEYMESLYGKKITRSPITPFYRKPRNLYNT
                     GQAICGGRGCLRACMINLEKRDVLGNKFDQPFRRRKPWKMDWSDTGVSPESQAKGEAD
                     "
     gene            complement(2255..3310)
                     /locus_tag="PLY56_10085"
     CDS             complement(2255..3310)
                     /locus_tag="PLY56_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21873.1"
                     /translation="MRLSIGYQLPDEDDSLVEIVRDYRESIAEVYFALPGHASGRSPL
                     GGAGGEGDPEARAILMEELAAISEMGVELVLLLNAACYGEEAISRAFAESIRKTLRDF
                     LAELPLAAVTTTSPFVARVIKEAFPELETRASVNMRVGTVKGMQYLADHFDGFCMQRE
                     YNRDPDQIEELKAWCDAHGKRLYFLANSGCMRSCSWQSFHDNLVAHEAGASTRDNVPL
                     GYPSPCWEFMARRENWVAYLQNTWVRPEDLRHYERWFSLAKLATRMHSNPRRVVAAYA
                     RGRFHGNLLDLTEPSYAPLLRGYILDNQRFPDDWFERTASCDKACHRCAYCESVLQQV
                     LVSVEELLRGVQGGSRC"
     gene            complement(3405..>3861)
                     /locus_tag="PLY56_10090"
     CDS             complement(3405..>3861)
                     /locus_tag="PLY56_10090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ21874.1"
                     /translation="HTAFNPVEPARAMPAVDHPADTPERLRRFGDLEPSGAGLWSTAP
                     DLIRLAQALLTPGKLLTPEVYRRHTELRPSLPAVGGDGVSRRTWGWNREIRPEFTRQP
                     ESGFFHGGATGTLLWIDPDADLIFVFLTNRWGSGNEHCFITLDALYGAA"
BASE COUNT          666 a         1333 c         1214 g          648 t
ORIGIN      
        1 cgcgcatatc ggcaactgcg tcatgcgcga cagctcgcac ccggcctatg gcgacgagca
       61 cccgcgcttc ggcgttgagg gcggcgagaa cggcgtcgcc gagctggccg acttcatgca
      121 ggagctgctc cgctgcggtt acctgaaggc cggcgagccg cggatcgtca gcttcgaggt
      181 gaagccgatc gccgcgtggg gcgagaccag ccaggtcgtc gtcgccaacg cgaagcgcgc
      241 gctccagaag gcctgggcgc aggtccagct ctaaatctaa ccggcctgcg gagggcccgg
      301 agagcccgca cgggttcccc gagtcctccg gggccgctct tggcgcgctc gtctatccgt
      361 cggagatccc gatggctgat cgcaagaggc agacgacgat ccgcaccgtg cgggaactcc
      421 tcttcaccca acccctcgct cgtctctaca gcagtccctg cgttggccgg gccggccaga
      481 cttccgacac gcaagagccc ttcgcggagg tgatcgctga ggagctactg cgcatccagg
      541 tggcacagct cctcggttcc gtgggcatcc ccccacagcg agcgatgccc tatcgccaag
      601 gccatgatgg caccgtcagc acgcccacca cccctcgaag tgccaccagc gagcgacgcc
      661 tagctatcgc gctctacaac tactcgcggg atcatggcgg cgactgtttt ggcctgctcg
      721 gccagccaat cgactacgag gtgccccttc aggcgcaacg gggcgcggga atcggcgaga
      781 tcgacctgct cacgcaagtg aaagaggaga gcccaggcat cgagaatgcg cgcggcgttt
      841 acgtagtcga gctgaagagg aaacgcgaga agagccccga gtcgctcttg cgctcggtgc
      901 tgcagatcgc cacttactac cagcagctcg accgcgagaa actcctgcgc gactatggcc
      961 tctatggcga cccgtccggc tacggcacgc acgagatccg caaagcggtc ctcgtctttc
     1021 gcggctccgc gcaggagagg gagatccagg cgatcaagaa gggctccctg ccgaacttgc
     1081 gccgcctaat tgaggacctc ggagtgcatc tgttcgtgat cgacgccgac gataccgtcg
     1141 tcgatcaggg caccggcccc taccgtccgg ttcttcaggg cggaggagcc ggcgtgcgca
     1201 gcgtcaagcg cgtgctcctc tgagcgcggc ccacgtcagt cggcttcgcc cttggcctgg
     1261 ctctcgggag acacgccggt atcggaccaa tccatcttcc agggcttgcg gcggcggaac
     1321 ggctggtcga acttgttgcc cagcacgtcg cgcttctcca gattgatcat gcaggcacgc
     1381 aggcacccgc gtccgccgca gatcgcctgg cccgtgttgt agaggttgcg cggcttccgg
     1441 tagaacggcg tgattgggct acgcgtgatc ttcttgccgt acagcgactc catgtactct
     1501 gggctgtcgt ggtctgggtc ggccacctgg ccgccgcgga aggcgatgtc gcacgcggcg
     1561 gtgtccagct ctccccactc cacctcgtgg ccggcgaggg tcaccttcac cgtcttcgtc
     1621 gcgctgatcg ccttgccagg gcactcgcgc acgcacgcca tgcagcggtt gcacagcttc
     1681 ggaccgttgt agatcgggtc cggttccagc tccgcctcgg tgaggacgac tcccaggcgc
     1741 tggcgcgggc cgaattgcgg ggtgaggaag accttgctgt agccaatctc gcccaggccg
     1801 gccaggaagc ctgcgatccg caggtggatc atcacatcgg gtgcggcgcg gcccggctcg
     1861 acaggccgac tatacccctc gcgcaggaag ccgttgccat cgatggcgcg ccagtcactc
     1921 tggtgtccaa tcggaatcgc ctccttgccg tggtcctcga tgaagcggca caggttgatc
     1981 acgaccaacg gcatgtagag gtaggtgaga ccgccgtagc ccatcgccga gtagttcgag
     2041 aagaaggtgc cctcctcaat cccgcgcagg ctcccgcgca tcacccggaa cgccatcacg
     2101 atcagcgagc gtgcctcggg catgatctgc cgcgggtcca tctgcagggg agcgccctcg
     2161 taacgatcca tcgaggcgat ccccaccaga tcggccccca gcgccttcgc ttccttcttg
     2221 accatttccg ctgtcagcat cttgaccctc cttgtcagca tcttgaccct ccttgcaccc
     2281 cgcgaagcag ctcctcgacg ctcaccagca cctgctgtag cacgctctcg cagtaagcgc
     2341 accggtggca ggctttgtcg cagctggccg tccgttcgaa ccagtcgtcc ggaaagcgct
     2401 ggttgtccag gatgtagcct cgcaggagcg gggcatagct cggctccgtg agatcgagca
     2461 ggttgccgtg gaaccgcccg cgggcatacg ccgccaccac cctcctgggg ttggaatgca
     2521 tccgcgtcgc gagcttcgcc agtgaaaacc accgctcgta gtggcgcaaa tcctccgggc
     2581 gtacccacgt gttctggaga tacgccaccc agttctccct ccgcgccata aactcccagc
     2641 agggactggg ataccccagc gggacgttgt ccctcgtgct ggctccggcc tcatgggcca
     2701 ccaggttatc atggaagctc tgccacgagc aggagcgcat gcacccgctg tttgccagga
     2761 aatagaggcg cttgccgtgg gcatcgcacc acgccttcag ctcctcgatc tgatccggat
     2821 cgcggttata ctcgcgctgc atgcaaaagc cgtcgaagtg atccgccaga tactgcatcc
     2881 ccttcaccgt gccgacgcgc atgttcactg aggcgcgcgt ctccagctcc gggaacgctt
     2941 ccttgatcac ccgggcgaca aagggagagg tggtggtgac cgccgcgagg ggtagctctg
     3001 ccagaaagtc gcgcagagtc ttccggatgc tctccgcgaa cgcccgcgag atcgcctctt
     3061 cgccataaca cgcggcgttg agtaggagca ccagctcgac gcccatctcc gagatggcag
     3121 ccagctcttc catcaggatc gcgcgcgcct cgggatcccc ctccccgccg gcgccaccga
     3181 gaggagagcg cccgcttgca tgcccaggga gcgcgaaata gacctccgcg atgctctcgc
     3241 gatagtcgcg cacgatctcg acgagcgagt catcctcatc tggaagctga tagccgatgg
     3301 acaggcgcat gactctctcc aaccccgccc cacccgggcg cgcggccacc agccgacatc
     3361 aaggcatgcc agctcgtcac accgtggccc gctccctccc ccggctacgc ggccccgtag
     3421 agggcatcga gggtgatgaa gcagtgctcg ttgccgcttc cccaacggtt ggtgaggaag
     3481 acgaagatga gatccgcgtc cggatcgatc cagaggagcg tgccggtggc acccccgtgg
     3541 aaaaagccgc tctccggttg gcgagtgaac tcagggcgga tctcccggtt ccagccccag
     3601 gtgcgccggc tgaccccgtc gccacccacg gcgggcagac tgggcctcag ctccgtgtgc
     3661 cggcggtaca cctcgggagt gaggagcttg cccggggtca ggagggcctg cgccagccgg
     3721 atcaggtccg gtgcggtcga ccagaggcca gccccagaag gctccaaatc gccaaagcgg
     3781 cgcaggcgct cgggcgtatc cgccgggtgg tccaccgcgg gcatggcgcg cgccggctca
     3841 acggggttga aagcggtgtg g
//
LOCUS       DAOSCV010000381         3858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14925_length_3858_cov_3.275046, whole genome shotgun sequence.
ACCESSION   DAOSCV010000381 DAOSCV010000000
VERSION     DAOSCV010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14925_length_3858_cov_3.275046"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="fliD"
                     /locus_tag="PLY56_10095"
     CDS             <1..1002
                     /gene="fliD"
                     /locus_tag="PLY56_10095"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOJ21875.1"
                     /translation="GGLTVGSDGKLQYDSARLTSALKENPTAVMRLFANYGETADPRV
                     KYIGCTTETVASGAAGYSVHITQVATRSRITAGVAQTEALAGDEQLTINGTTILLTSG
                     MTQADVIAAINAKSSLTGVIARATGADGTGEGNYLTLEASQYGSAYTLSVVSNMSNGG
                     AMPVGNRTGIGSMLVTESDAAGEAGTGSGARGVNVAGTINGEPATGSGQILTGKVGNA
                     TTSGLMLQITSQTTGAHGSVVFTRGVGGELERIIQAAIEGAESTVEQARSSVQAEIDA
                     LTEEMKLADERLAALQERLRAQFTAMESTLSRLQNQSLQLAQQIASLSGQKSSTRGM"
     gene            1018..1431
                     /gene="fliS"
                     /locus_tag="PLY56_10100"
     CDS             1018..1431
                     /gene="fliS"
                     /locus_tag="PLY56_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOJ21876.1"
                     /translation="MQNPYSLYQEQRIQHASPGERVLMLYDGALRFLRGARLAMETGN
                     AAGQSHNIDRAQSILLGLISSLNIQEGGELAICLLRVYEYCYNRLCDASANDDMTAIT
                     EVAALLNNLRGAWADAAAQVERGQEAETAPLAMTA"
     gene            1480..1842
                     /locus_tag="PLY56_10105"
     CDS             1480..1842
                     /locus_tag="PLY56_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21877.1"
                     /translation="MKTLLALYQHLTELCHREAQLIAAEDLVGLDKLLDEKDSVLQKI
                     ILEQARIQATGEREGLDQVLMAAEAAAAAEAAAARGLRLNLDALRQRLRQAGEGRAVV
                     RSYRPDRYPTSRMDHYSA"
     gene            complement(2071..3639)
                     /locus_tag="PLY56_10110"
     CDS             complement(2071..3639)
                     /locus_tag="PLY56_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ21878.1"
                     /translation="MRVIILQPPYPHEGTENAALRCIEWMLQHLDALRPGDQDLILLP
                     EYANAPGLEEKSVLRAFVQTHGLEFASRLAAKSRELGSLLAAGLVSYSGGTWENRAFL
                     FSPKGETVASYAKAHLTAVERERMGLTPGASVVVAEHAGVRFGFATCLDIYLPEFTEA
                     LAARGVDLILHSSYQRSEEPERIRLLCRARALDAGAYVLRSGYAMGRADRGGSSLVAA
                     PDGNILADAGAEPGLVTCALEPKQKFAPPASHGQPPVEHRQRTESLRRPGIYRPVADR
                     AAVLLATRLPWVCAHRGLSTVCPENTLPAFGAAIAVGAQEVEMDLWMTRDGVAVICHD
                     PRLDRTTDGQGVIQEMTWEEIRRFDAGVRFGERWRDLRIPRFEEVLDVVDGRAGLNLH
                     VKDPGPDGALVRMMRDELHKRGLVELAYLGGNEKTLRAAREIAPEIPRACLDRQSEPN
                     RQIDLAVEYGCRRIQFYRHATEEHFRRAHDLGIVCNLFWSDDPAEARAYVERGVDVVL
                     TNAAHQVTRATLLA"
     gene            3626..>3858
                     /locus_tag="PLY56_10115"
     CDS             3626..>3858
                     /locus_tag="PLY56_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21879.1"
                     /translation="MITRIVGLFSSVLARLRQTRKSGRASCRESVAGACSPAGNTRWG
                     CLAEPPGTHTLLLQPAVFPLGCSRGRFPVASSC"
BASE COUNT          678 a         1249 c         1328 g          603 t
ORIGIN      
        1 gggggcctca ccgtgggcag cgatggcaag ctgcagtacg acagcgcccg gctgacgagc
       61 gcgctgaagg agaatcccac ggcggtgatg cggctctttg ccaactatgg cgagacggcc
      121 gatccgcgcg tcaagtatat cggctgtacc accgagacgg ttgcttcggg cgctgcgggc
      181 tactccgtgc atattacgca ggttgccacg cgtagtcgga tcaccgcggg cgtggcgcag
      241 accgaggcac tggctggcga cgagcaactg acgatcaacg gcacgacgat cttgctgacc
      301 agcgggatga cgcaggcgga tgtgatcgcg gcgatcaatg ccaagagctc gctcaccgga
      361 gtcatcgcca gggcgacggg agccgatggc acgggcgagg gcaactatct gacgctggaa
      421 gcgagccagt acggctcggc ctacaccctc tcggtggtct ccaacatgtc gaacggtggc
      481 gccatgccgg tgggcaaccg caccggcatc ggttccatgc tggtcacgga gagcgatgcg
      541 gccggcgaag cgggcaccgg ctcgggagcg cgtggcgtga acgtggccgg cacgatcaat
      601 ggcgagccgg ccaccggatc gggccagata ctgaccggga aagtggggaa tgccaccaca
      661 tccggactga tgctgcagat cacgtcgcag acaaccggcg cgcatgggtc tgttgttttc
      721 acccgcggcg taggtggaga actagagcgg atcatccagg cagcgatcga gggcgccgag
      781 tcgacggtgg agcaggcgcg ctcatcggtc caggcggaga ttgatgcgct gacagaagag
      841 atgaagctcg cggacgagcg gctggccgcg ctccaggagc gccttcgggc gcagtttacg
      901 gcgatggaga gcaccttgag ccggctccag aaccagagcc tgcagttggc gcagcagatt
      961 gcaagccttt ccggccagaa gagcagcacg cgtgggatgt agcaagggag gagagagatg
     1021 cagaatccat actcgctgta tcaagagcag cggatacagc atgcatcgcc gggggagcgg
     1081 gtgctgatgc tctacgatgg ggcgttgcgg ttccttcggg gggcgcgcct ggccatggag
     1141 acggggaacg cggcggggca gagccacaat atcgatcgtg cgcaaagcat cctcctgggc
     1201 ctgatctcgt cgctgaatat ccaggagggg ggcgagctag ccatctgcct gttgcgagtg
     1261 tatgagtatt gctacaaccg gctctgtgat gccagtgcca acgatgacat gactgccatc
     1321 acggaggtgg ccgccctcct taacaacctg cgtggcgctt gggcggatgc cgcggcccag
     1381 gttgagcggg ggcaagaagc cgagacggct cctttggcga tgaccgcctg agccagggga
     1441 aagcgcgtcc ccgatcgtat cgcgaagggt ggcctactga tgaaaacgct cttggctctc
     1501 tatcagcacc tcacggagct gtgtcataga gaggcgcaac tgatcgcggc cgaggatctg
     1561 gtcggtcttg acaagctcct ggacgagaag gatagcgtcc tccagaagat tatcttggag
     1621 caggcgcgca tccaggccac cggcgagagg gaagggctgg atcaggtgtt gatggccgcc
     1681 gaggcggcgg ccgccgctga ggcggcggcg gcgcgtgggc tgcgcctgaa cctggacgca
     1741 ctgcgccagc ggcttcgcca ggcgggcgaa ggccgcgccg tggtccgctc ctaccgcccg
     1801 gaccgctatc ctacctcccg gatggatcat tactccgcct gaggccctct ggggaggtgc
     1861 tagcgggcgg cactcccggg tcgcctcccg gccgggttgc tcgcatccct cggggccgag
     1921 atcaggccag ccgcgcggca ccggcggagg gctccgccgg ctccatcccc tcagcccgag
     1981 ggttcccaac cccggagctg gcttggggcc ggagggcagg gtagggtagg agaaccgccc
     2041 ccagcccccc ggcgttcgtc actcgcttcg ctacgccagc aaagtggcgc gagtcacctg
     2101 atgggcggcg ttggtcagga ccacatccac tccgcgctcc acgtaggcgc gcgcttccgc
     2161 cgggtcgtct gaccagaaaa ggttacacac gatgcccaga tcatgggcgc ggcggaaatg
     2221 ctcctccgtc gcatgccggt agaactggat gcgccggcac ccgtactcca ccgcaagatc
     2281 aatctgccgg ttcggctcgc tctggcggtc gaggcaggca cgcgggatct cgggagcgat
     2341 ctcgcgcgcg gcgcgcagcg tcttctcgtt gccccccaga taggcaagct ccaccaaccc
     2401 gcgcttgtga agctcgtcgc gcatcatccg caccagcgca ccgtcgggcc ccgggtcttt
     2461 gacgtgaaga ttgagccctg cccggccatc gactacatcg aggacctcct cgaagcgggg
     2521 aatccgcagg tcgcgccacc gctcgccgaa ccgcacgccg gcatcgaacc gccggatctc
     2581 ctcccaggtc atctcttgaa tcactccctg gccgtcagtg gtgcggtcga ggcgcgggtc
     2641 atggcagatc acggccacgc cgtcgcgggt catccagaga tccatctcga cctcttgcgc
     2701 gccaacggcg atggccgccc cgaaagcagg cagcgtgttc tccgggcaga cggtgctgag
     2761 cccacgatgc gcgcacaccc acgggaggcg cgttgccagc aacaccgccg cgcggtccgc
     2821 caccggacgg tagatgccgg gacgccggag tgactccgtg cgctgtcgat gctccaccgg
     2881 cggctggccg tgggaagcgg gcggcgcgaa cttctgcttc ggctcaagcg cgcaggtcac
     2941 gaggcccggc tcggcccccg cgtcggcaag gatgttgcca tccggcgcgg cgaccaggga
     3001 ggatccgcct cggtcggcac ggcccatggc gtagccgctg cgcaagacgt acgccccggc
     3061 atccagcgcc cgtgcacggc agagaagccg gatccgttcc ggctcctcgg agcgctggta
     3121 actggagtga agaatgagat cgacgccccg cgccgcaagc gcctcggtga actcaggcag
     3181 atagatatcg aggcaggtgg caaatccaaa gcgcaccccg gcgtgctccg cgaccacgac
     3241 ggaggctccc ggagtgaggc ccatccgctc cctctcgacg gccgtgaggt gtgctttggc
     3301 ataactggcc accgtctcac ccttggggct gaaaaggaag gcgcggttct cccaggtgcc
     3361 accggagtac gaaaccaggc cagccgccag gagcgagccg agctcccggc tcttcgcggc
     3421 cagccggctg gcgaactcca ggccgtgcgt ctgaacgaat gcccggagaa cgctcttctc
     3481 ctcgaggccc ggggcgttgg catactcggg aagaaggatc agatcctggt cgccagggcg
     3541 cagggcatcc aggtgctgaa gcatccactc tatacaacgg agcgcggcat tctcggtgcc
     3601 ctcgtgcggg tagggcggct gaaggatgat cacacgcatt gttggcctct tctcctccgt
     3661 gctcgcccgg ctccgccaaa cgcggaagtc ggggcgtgcc tcctgccgcg agagcgtggc
     3721 gggcgcttgc tcgccggcag ggaatacgcg atggggctgc ctggcagagc cgcctggcac
     3781 ccacactctc cttctccagc cggccgtctt tcccttggga tgctcgcgcg ggcggtttcc
     3841 tgtggcgtct tcctgcca
//
LOCUS       DAOSCV010000382         3854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14941_length_3854_cov_3.580153, whole genome shotgun sequence.
ACCESSION   DAOSCV010000382 DAOSCV010000000
VERSION     DAOSCV010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14941_length_3854_cov_3.580153"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PLY56_10120"
     CDS             complement(<1..344)
                     /locus_tag="PLY56_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ21880.1"
                     /translation="MATVSATAKESFRTDGLLVLQAQAGDERAFELLYERHERRVYHL
                     ALGMLHSVEDAEDVTQETFLNAYRALPQLTDGQAFHAWTTRIAVNLCRRSRARRKAGE
                     TILSLDAESTETG"
     gene            complement(372..1016)
                     /locus_tag="PLY56_10125"
     CDS             complement(372..1016)
                     /locus_tag="PLY56_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ21881.1"
                     /translation="MGVEKRRRLTAEERREKILDAALEVFSERGFAAATTRELAARAG
                     ITEAIIYRHFRSKEEVLRGVVERDSFLQHVPQFVSVDAARPLRDQLLDLGQNWRRFVR
                     ERRSLVTLLLGEMVRSEEMARLLRETMIHDAVSLVVRALRPQVSESVSEERLTMAVRL
                     FYASLLCHFVLEERLGAEEDPHAQARLETTVDLLLDALGAPSGSSGRAPAVRNP"
     gene            complement(1210..1668)
                     /locus_tag="PLY56_10130"
     CDS             complement(1210..1668)
                     /locus_tag="PLY56_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HOJ21882.1"
                     /translation="MRYLVWIALLAGTLSAALAAPAEKEAPMTATLRVTPTRARPGEK
                     FELRLTVTNNGQREERLLFRSGQKFDFTVTRDQKVVWRWSHGRAFTMALEQITLKPGE
                     SRTFEAVWEQKDNAGKEVPEGEYLVTSVITAHPEIRAAPVKVRVAVAGPK"
     gene            1853..2335
                     /locus_tag="PLY56_10135"
     CDS             1853..2335
                     /locus_tag="PLY56_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21883.1"
                     /translation="MDVWRRIGIAIGLAGTLLLGGVGCISRPDTQVRGYRGTPPQTMP
                     EAQPEKDPAKPPGSEGFRIPVHSNTPEGRELCDRMILASEGILADAQIIDNRDLHVVL
                     GSKANPDDLPALMRTLVLIMHHSFPEENVTVRAYDRSGRVLVLATVDVQEGTVTYSFP
                     "
     gene            2459..3217
                     /locus_tag="PLY56_10140"
     CDS             2459..3217
                     /locus_tag="PLY56_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOJ21884.1"
                     /translation="MKAILWCTFGLAGLLWATGLAGQGAPIERVLLDTDTMPVVQEGR
                     VLAPVRAVAEVFRAQVEWLPGAREVMVRHAGREVRLTAGSRSARVDRRLVTLDVAPAI
                     AEGRLLVPLRFLAEALGIAIRYEAATHSVLVDTGRAGEALRVLPLFTVRGGIHVLTPQ
                     PDAQISSPVRVHGQANTFEGNVVIEVQSSDGQVLGRGITTGAMGSYHPFTADVAFQLP
                     PGMERGRLFLYSSGGREGEILHPVSIPVRFASPG"
     gene            3625..>3854
                     /locus_tag="PLY56_10145"
     CDS             3625..>3854
                     /locus_tag="PLY56_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ21885.1"
                     /translation="MSTLLGSNLRRLRTAKGYTQEAVADAARISVAAYRNYETGREIP
                     KVNTLLAIARALGVSLNDLVTEARPLTAVRFRV"
BASE COUNT          645 a         1301 c         1241 g          667 t
ORIGIN      
        1 ccggtctccg tgctctcggc atccagcgag agaatggtct cgccggcctt ccggcgcgcc
       61 cggctccggc ggcagaggtt cacggcaatg cgagtggtcc aggcgtggaa ggcctgtcca
      121 tcggtcagct gtggcaacgc gcggtaggcg ttgaggaacg tctcttgggt gacatcctcc
      181 gcgtcctcga cgctgtgcaa catcccgagc gccaggtgat agacgcgacg ctcgtgccgc
      241 tcatacagca actcgaacgc tcgctcatcg cccgcttgcg cctgaagcac caggaggcca
      301 tccgtacgga agctctcctt cgccgtcgct gaaaccgtcg ccatctttcc tccaccagac
      361 cgcatcgcga ctcacggatt cctgactgcc ggggcccgcc ctgaagaacc cgacggtgcc
      421 ccaagcgcat cgagaaggag gtccaccgtc gtctcgaggc gggcctgcgc gtggggatcc
      481 tcttcggcgc cgaggcgttc ttccagcaca aagtgacaca ggagtgaggc gtagaaaagg
      541 cgcaccgcca tggtcagccg ctcctcggag actgattcgc tcacttgcgg gcgcagggcg
      601 cgcacgacca gtgaaaccgc atcgtggatc atcgtctcgc ggaggaggcg cgccatctcc
      661 tccgagcgaa ccatctcgcc caggagaagc gtgaccagag agcgacgctc gcggacaaag
      721 cggcgccagt tctggcccag gtcaaggagc tgatcgcgca gtggccgggc ggcatcgacc
      781 gagacaaact ggggcacatg ctgcaggaag ctatcgcgct ccaccactcc gcgaaggacc
      841 tcctccttgc tgcgaaagtg gcggtagatg atcgcctcgg tgatccctgc gcgcgctgcc
      901 agctcgcgcg tggtcgccgc tgcgaaccct cgctcggaga agacctccag ggctgcatcc
      961 agaatctttt cccgccgctc ctccgccgtc agccgccggc gcttttcgac acccataatc
     1021 ccccgcaaac aataagtaaa cgcttactta catcatacca acaggcgcgc ctcctgtcaa
     1081 gggaataggc cggccgccat cagagccacg ctgcgcgagg gcttgccgcg caacgggttc
     1141 cagaggcgac gggcaggccc caacggcgct cccacggcgc gccatccgcg ggggatggga
     1201 ggcgaggcat cactttgggc cggccacggc gacccgcacc ttgacggggg cagcacgtat
     1261 ctccggatgg gccgtgatga cgctggtgac gagatactcg ccttcgggca cctctttccc
     1321 ggcattatcc ttctgctccc aaaccgcctc gaacgtccgg ctttcgccgg gcttcagggt
     1381 gatctgctcg agtgccatgg tgaaggcacg gccatggctc cagcgccaca ccaccttctg
     1441 gtcgcgtgtc accgtgaaat cgaacttttg gcccgatctg aagaggaggc gctcctcgcg
     1501 ctggccgttg ttcgtgacag tcagccgcag ctcgaacttt tcgccgggcc gggcccgtgt
     1561 gggggtcacg cgcagggtcg ctgtcatggg cgcctccttt tccgcgggag cggcaagcgc
     1621 cgcgctcaga gtgccagcca ggagcgcgat ccagacgaga taccgcatcg ctacctctct
     1681 cccctcttcg gattcctcaa atcttccttc ggcgtcagcg aggtcgtctc ctggggtttc
     1741 cgcgcgctgg gggccgggta catcggggca agagagattc gctccttcac aggggtgttg
     1801 gggcgcgcgc cgcggcccgg gttccggcgg ccgggcttaa ggaggcaagc cgatggacgt
     1861 ctggcggcgg atcggcatcg cgatcggcct ggcaggaacc ctactactcg gaggagtcgg
     1921 gtgcatctcc cggccagaca cccaggtgcg cgggtaccgg ggcacgcccc cgcagaccat
     1981 gccggaggcg cagccagaga aggatccagc caagccaccg ggctctgagg gcttccgaat
     2041 cccggtgcac agcaacacgc cagaggggcg ggagctgtgc gaccgcatga ttctcgcctc
     2101 ggagggcatc ctggccgacg cccagatcat cgacaaccgt gatctgcatg tcgttctggg
     2161 ctccaaggca aaccccgacg acctccccgc gctgatgcgc acgctggtcc tgatcatgca
     2221 ccactccttc cccgaagaga acgtgaccgt gcgcgcctac gaccgttctg gccgggtgct
     2281 ggtgctcgcc acggtcgatg tccaagaggg cacggtaacc tacagcttcc cttgaggggc
     2341 gcggcgtgcc ggacacaacg gggtcttccc agatcgcggc aggtgaatcc catcgtccgc
     2401 tccgccgcga gagcggactc ccggttcacc gtggcgggaa cggggcacta ttgcctgtat
     2461 gaaggcgata ctttggtgca ctttcggcct cgcggggctc ctctgggcga ccggcctcgc
     2521 tgggcagggc gctcccatcg agcgggtctt gctcgacaca gataccatgc cggtggtgca
     2581 ggaggggcga gtgctggcgc cggtgcgcgc ggtggcagag gtgttccgag cgcaggtgga
     2641 gtggttgcca ggggcgcgcg aggtgatggt gcgccacgcc ggccgggagg tgcgtctgac
     2701 cgctggctcg cggagtgcgc gcgtggatag gcggctcgtc accctcgacg tggccccggc
     2761 tatcgcggag gggcggctgc tggtaccgct ccgctttctg gcggaggcgc tgggaatcgc
     2821 catccggtat gaggccgcca cgcacagcgt gctcgtcgat acgggccgcg cgggcgaggc
     2881 tttgcgcgtg ctcccgctct tcacggtgcg cggcgggatc cacgtcctga cgccccagcc
     2941 ggacgcccag atctcgtccc cggtgcgcgt gcatgggcag gccaacacct tcgagggcaa
     3001 cgtggtgatc gaggtccaga gttcggacgg tcaagtgctg gggcgcggca tcaccaccgg
     3061 cgcgatgggc agctaccacc cctttacggc cgatgtggcc ttccagctgc cacccgggat
     3121 ggaacgcggt cggctcttcc tctactcatc cggcgggcga gagggcgaga tccttcaccc
     3181 cgtcagcatt cccgtgcgct ttgccagccc cggatagggc tcaggagagc gcgggtggca
     3241 agccatcatt ctttgcaggt ccgactcccg catgcgctac actctgcctg gcttcatgcc
     3301 tggcaagtgc ccggcgcgca tactctcagg cgctacggca agacgctctg ccacagcctc
     3361 gggcgatagt gctttgtcca tgcgctgttc ctccctggcc gagcctagaa ccctatgccc
     3421 atcaaggtcg gccaaagcgc gttgtggttg tgcccttcca gggatgcgga tggctctttc
     3481 gcaatcgcct tccagcacct ccggcttgga gactgcctgg atgagcccgt ccgtgatcat
     3541 tacattcacg ttatgcacac aataacacta tttacaagaa ttgtagaaag tgctatccta
     3601 tacttgacag gaaaggaggc ccccgtgtcc accttgcttg gcagcaacct gcgaaggctg
     3661 cgcacagcga aagggtacac ccaggaagcg gtggccgatg ccgcccgcat cagcgtcgcc
     3721 gcttaccgaa actacgagac cggccgggaa atcccgaagg tcaacacgct cttggccatc
     3781 gcgcgcgcgc ttggcgtctc cctcaacgac ctcgtcaccg aggcgcgccc cctcacagcc
     3841 gttcgcttcc gggt
//
LOCUS       DAOSCV010000383         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14955_length_3851_cov_3.725764, whole genome shotgun sequence.
ACCESSION   DAOSCV010000383 DAOSCV010000000
VERSION     DAOSCV010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_3851_cov_3.725764"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..780)
                     /locus_tag="PLY56_10150"
     CDS             complement(97..780)
                     /locus_tag="PLY56_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOJ21886.1"
                     /translation="METRSSGWVAEAGAVFLKDLQQELRNRYALNAMALFAITTLVVV
                     GFAVGSQALPASVVAALLWVVLLFTAVASLSRVFLREEETGTALALRLAVEPEHVYLG
                     KLLFNVVLLGAMALVVAPLFLAVMQARVGNPGLLALVLLLGCIGLAAASTIVAAIIAR
                     AAARGALFGGLAFPLLLPVLIAATGGTTRALEGAPLGAAWAELRLLLSFGVVMITASL
                     LLFEHVWNE"
     gene            complement(749..1402)
                     /gene="ccmA"
                     /locus_tag="PLY56_10155"
     CDS             complement(749..1402)
                     /gene="ccmA"
                     /locus_tag="PLY56_10155"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HOJ21887.1"
                     /translation="MRSPDGGPEVPAEVFLAVRDLAKSFGRLGLFRGVSFELSPGEAL
                     VVTGRNGSGKSTLVRILCGLLRPTRGSIEYRVGERLVPGRDVKRLVGLVSPDLVLYEE
                     LTLLENLRFFARVRGLRRSDMELQTLLTQVGLESEGARRAGTLSTGMRQRLRFAHALL
                     HEPPVLLLDEPTANLDDAGVAMARACMARQRSRGILVVATNNREELELGDQILRLGG"
     gene            complement(1709..2041)
                     /locus_tag="PLY56_10160"
     CDS             complement(1709..2041)
                     /locus_tag="PLY56_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21888.1"
                     /translation="MSTTRTNTETPCPWCRDGRIVLHDPEEEWWVTEEGSAASDSYTG
                     KFPAGIRRKRFRTPPVEWFSCTNCVMTGTVGYLTTVLAQAPQQRSSDTSRGSCSAVKR
                     GSLASAFS"
     gene            complement(2275..2940)
                     /locus_tag="PLY56_10165"
     CDS             complement(2275..2940)
                     /locus_tag="PLY56_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21889.1"
                     /translation="MRTHCTAARAHRGFTLIELLVVIAIIAILAAILFPVFARARENA
                     RKATCQSNLRQIGMATMMYAQDYDETMPKCYLGLTYSIRWYYQSTSNPGMLFPYIKNS
                     RVFKCPSGGCYGANRDILLSNGQGPPLPVASLTAPAVTVMFADVTLWYCDPVTGLGGN
                     DAWGLGLSKWSQFESRLGHGCYGGGCMLARHIGMAMVCFADGHVKAMDPLTTERAPYQ
                     WAP"
     gene            3650..>3851
                     /locus_tag="PLY56_10170"
     CDS             3650..>3851
                     /locus_tag="PLY56_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21890.1"
                     /translation="MHARMSMRWTVTLFTAFFLAVSLSLVLGAMINRSRQQWKEDSLL
                     QARAAAGLFARTSRDALRRVRHQ"
BASE COUNT          713 a         1321 c         1193 g          624 t
ORIGIN      
        1 cgcggaagcc cgcggccggg cccacgccga tgaaagccgc cgcgatcacg ccggccatcc
       61 acgcgccgca cactatccgc cacccggtcg agagccctat tcgttccata cgtgctcaaa
      121 gagaagcagc gacgccgtga tcatcaccac gccgaacgag aggagcaatc ggagctccgc
      181 ccatgctgcg cccaagggcg cgccctccag cgcccgcgtg gtgccgccgg tcgcggcgat
      241 caggacgggc aggagcagtg ggaacgccag cccgccaaac agcgcgcctc gcgccgccgc
      301 gcgcgcgatg atcgccgcga cgatggtgct cgccgcggca agcccgatgc acccaagcag
      361 gaggacaagc gccagcaggc ccgggttccc cacgcgcgcc tgcatcaccg ccagaaagag
      421 cggcgccacc accagcgcca tcgcgcccaa gaggaccacg ttgaacagca gcttgcccag
      481 atagacatgc tccggctcca cggccaggcg cagcgccagc gccgtgcccg tctcctcttc
      541 gcggaggaag acgcgcgaca ggctggccac cgcggtgaag agaaggacca cccacaagag
      601 cgccgcgacg acactagccg ggagcgcctg gctccccacg gcgaagccga cgaccaccag
      661 cgtggtgata gcaaagagcg ccatcgcgtt gagcgcgtag cgattgcgca gctcctgctg
      721 cagatccttc aggaaaaccg cgccggcttc agccacccag ccggaggatc tggtctccaa
      781 gctcgagttc ttcacgattg ttggtcgcca ccaccagaat gccccgggag cgttgccgcg
      841 ccatgcacgc gcgcgccatc gccacccccg cgtcatccag attggccgtc ggctcgtcca
      901 ggaggagcac cgggggctcg tgcaacaggg catgggcaaa ccgcaggcgc tggcgcattc
      961 cggtggagag cgttcccgcc cggcgcgcgc cctcgctctc caggccaacc tgggtgagga
     1021 gggtttgcag ctccatgtca ctccgccgga ggccgcgcac gcgcgcgaaa aaccgcaggt
     1081 tctccaggag cgtcagctcc tcgtagagca ccaggtccgg gctcaccagg ccgacgagcc
     1141 ttttcacatc gcgccccggt accagccgct cccccacgcg atactcgatg ctgccccgcg
     1201 ttggacggag gagcccacag aggatgcgca cgagggttga tttgccggag ccattccgcc
     1261 cggtgaccac cagcgcctct cccggcgaca gttcaaagct cacgccacgg aagagtccaa
     1321 gtctcccaaa gctcttggcc agatcccgga cggcgagaaa gacctccgcc ggaacctcgg
     1381 ggccaccatc cggtgatcgc atcgctctac cggtcgcgcg tccttcttgc ccttccacca
     1441 ccgctggcat ctcgggggca ttatagcatg aacattctgc gagcgtcaaa aaaccggaat
     1501 gccatccgcg ggacggcact ctgaacggtc attgggggat ggccgggcgc ttcatacgtg
     1561 tcgtgcaagg atagcgcgcc cgagtttggg gaaggatcgt ccatgggtct tgtgtggagg
     1621 cccaggcggg ccagcggcgg tccgcctggc caccaccggg cctcctcgat gggcgtgatc
     1681 catggacctc ctcttctggg gaaaagccct atgagaacgc gctcgcgagc gagccacgct
     1741 ttacggcgct acaggagccg cgcgaggtgt cgctgctgcg ctgttgtggc gcctgcgcca
     1801 acaccgtggt caggtagccc accgtgccgg tcatcacgca gttcgtgcaa gagaaccact
     1861 cgacgggtgg tgttcggaag cgtttccgcc gaatgccagc ggggaacttg ccggtatagc
     1921 tatctgaagc agcactccct tcctccgtca cccaccactc ctcttctgga tcgtgcaaca
     1981 cgatccgccc atctctgcac cacgggcacg gtgtctcggt attcgttctg gtcgtggaca
     2041 tctccgtccc tccctcatcc cgggcgattg cccggcgaca cgccactccc aatagctact
     2101 actcctttga cgaccaccgg gcaagaaaag agcgcctgat ccgacgcagg agccattaag
     2161 atcctgtgaa ggtggcgctg gagagggttg ccttcgccgc gcggcgtggt acttttcgat
     2221 ggggaccgcc ccggagccgt ggggcagtcc cccttcaatg ccgggttgga aagatcaggg
     2281 cgcccattga tatggcgctc tttccgtcgt cagcgggtcc atcgctttga catggccatc
     2341 tgcgaagcag accatggcca tcccgatgtg ccgggccaac atgcagccac cgccatagca
     2401 gccgtgccct aagcgcgact cgaactgcga ccacttcgag aggcccaggc cccaggcgtc
     2461 gttccctcca agaccagtca ccgggtcgca gtaccacagg gtgacgtccg cgaacatcac
     2521 cgtcacagcc ggtgcggtga gcgaggcgac cggcaatggg gggccctgac cgttggagag
     2581 aaggatgtca cgattggccc catagcagcc cccggagggg catttgaaca ccctggagtt
     2641 cttgatgtaa ggaaagagca tgcctggatt gctggtactc tggtagtacc accggatgga
     2701 gtaggtgagg cccaggtagc acttgggcat ggtctcatcg tagtcctggg catacatcat
     2761 cgtcgccatg ccgatctgcc tgaggttgct ctggcaggtc gccttgcgcg cgttctcgcg
     2821 cgctcgggca aacaccggga agaggatcgc cgccaggatg gcgataatcg cgattactac
     2881 cagcagctcg atcagcgtga acccgcggtg ggctcgcgct gctgtgcagt gcgtccgcat
     2941 ggtcgtgccc tcccaggggt gccccggtcg ccggctggct gaaaggtggg cgccacccgc
     3001 gaggttggat agatctggca tagcacgccg agccaggccg cgacgggcac ctgtcactcg
     3061 cattatacgt aagagcgaaa cgactgtcaa ccgcgggtcc actatccggc caagcgcccg
     3121 cactgccggc gctaccgcgt cgataccgcg aatgaagcgg acggacacgg gtggaaaggc
     3181 acgcgcccgg gcgggacggc tcccatatca tgttggagac actccggctg aaaccggcgg
     3241 ggaaccaagt gaacttcgac tgcgtctata tagcagccat atatcgctcc cggaagtaaa
     3301 agctcgtccg gttcgtctct gctgagggtt cgcagaacgt cttccctgct cacggcgcac
     3361 ccggtctctt cttggccaag gccttcgggc ccacacctac cccgtggccg gccgctcgta
     3421 gggcaccacc gtcgtcagaa acacgccgaa ccccaggttc tagccattcc gtgcttccct
     3481 ggagcccaga cgacctgcca aggggcaccg tgcgcctgtt ccacacgcga gaacctcgcc
     3541 ttggaggagg tagaggggat ttggagagaa ccccatcggg tcctcatccg cctcctgcca
     3601 tcgcctccga aagtagagag tggaataggg ccgatgcccg gagatttgca tgcacgcccg
     3661 aatgtccatg cgttggacgg tcacgctctt cacggccttc tttctcgcgg tctcgctcag
     3721 cctggtgctc ggggcgatga tcaacaggtc gcgccagcag tggaaggaag actccctcct
     3781 ccaggctcgt gccgccgcgg gtctttttgc gcgcaccagc cgcgatgccc tgcggcgcgt
     3841 gcgccaccag t
//
LOCUS       DAOSCV010000384         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_14967_length_3849_cov_3.680812, whole genome shotgun sequence.
ACCESSION   DAOSCV010000384 DAOSCV010000000
VERSION     DAOSCV010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14967_length_3849_cov_3.680812"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLY56_10175"
     CDS             <1..1091
                     /locus_tag="PLY56_10175"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOJ21891.1"
                     /translation="HVNELQHDYIYLAPDQILDGNLLERYPVLYLPFTVAESEALVQK
                     LDAYLQDGGVLMGDLRCLRTDECGKPYAGPGPLRQLFGVERTDGRVEYGPTTIQVTEA
                     AAGIDLRGKELSLYGRESLRVVEGKPLARHATGEPALIVRPHGKGVAIYLNFSLPEYD
                     VTTRELIRQILARAGIEREIVAENPKGETPPRCYERNTFTRGPIAVHAFIRDHRRCTD
                     SDPVRFRFGKPTHLYDMRAGRYLGHVAETEAVVPPGQTALYACLPYQVTKVTVTLPSK
                     VRGGSVLTARASISAGEARPGDHIFHIQVIDPAGNEVRAYTTNALAPGGQLDLPLSFA
                     WNDPPGAWEVVVRDVLTGVSGAAGFTLE"
     gene            1234..>2036
                     /locus_tag="PLY56_10180"
     CDS             1234..>2036
                     /locus_tag="PLY56_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21892.1"
                     /translation="MKKGFVFSCIPTKRGNGTPMTPEDRLRLAKDAGFQGVEISTMDD
                     LGECERLASQARSIGIEIHSVMASGHWSFPLSSPDEETRQKGVANIRQSVDTAKVSGA
                     DTVLVVPAVVNEQTPYELAWDLALKSMRELAPYAEKNGICLAVENVWNKFLLSPPEML
                     RFIAACESPAVAAYFDVGNILIYGYPDQWIRSLGSTLRKVHFKDFDIQTRTFRNLLQG
                     NVPWDRVRQALLDVGYTDYCSVELPAYQSYPDQFIYDCSKQVDRIFAGE"
     assembly_gap    2037..2136
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2328..>3849)
                     /locus_tag="PLY56_10185"
     CDS             complement(2328..>3849)
                     /locus_tag="PLY56_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ21893.1"
                     /translation="GMSLQQVQNVIFQTLEGHLRAILGTLTVEDINANRQAFANRMMD
                     EAAVDLKKMGVGIDVLTIQQISDDQGYLDSLGQRRTAEVKRDASIGRAEAERDAEIGQ
                     AKANRDATIQASSARQAGEIAKAEAEAKIAAAQRDLEVQRAQYMAQVESERAKASQQG
                     PLAEAQARQAVVMEEVRIEQVRTQAQIAVQEQEALRKEKELVASVLKPAEADRQAAVI
                     KAEADRQAAIITAEGAQRAAVIKAEGDQRAQVALAEGEREARIARAEAQRKELETAAA
                     GQAAQVEQVGNAEASVIQAKGAAEAERIRQTGEAEAAVALAKGKAEAEAARLKLLAEA
                     EGLRQKAEAWKQYGEAAMLQMVVDKMPEIISAMSGPLQAVGNPLAGIDKVVMIDAGNN
                     GATNMQKFAGNVPAIAFSFMEQLGEVTGLDLRSILGRALQTKPADGDGKPARAETQAP
                     TPAPVAEPAPSAPKPAAPAPTPAAAAAKPAQKMTPLTPVAGAQDGELPATLDGKKA"
BASE COUNT          592 a         1273 c         1198 g          686 t
ORIGIN      
        1 agcatgtgaa cgagctgcag catgactaca tctatctggc gcccgatcag atcctggatg
       61 gcaatctgct cgagcgctac ccggtgctct atctcccgtt caccgtcgcg gaatccgagg
      121 cgctcgtcca gaagctggat gcctatctcc aagacggtgg cgtgcttatg ggcgacctgc
      181 gctgcctgcg cacggatgag tgtggaaagc cctacgccgg tccgggtccc ctgcgccagc
      241 tgttcggcgt cgagcgaacg gatggccgcg tggagtatgg gccaacgact atccaggtga
      301 ccgaagccgc cgccgggatt gacctgcgtg ggaaggagct gtcgctttac gggcgcgaaa
      361 gcctgcgcgt cgtcgagggg aagccactcg cccggcatgc caccggcgag ccggcgttga
      421 tcgtgcgccc ccatgggaag ggcgtggcga tctacctcaa cttcagcctg cccgagtatg
      481 acgtgaccac gcgggagctc atccgtcaga tcctggcgcg cgccgggatc gagcgcgaga
      541 tcgtggcaga aaaccccaag ggagagacgc ccccgcgctg ctatgagcga aacaccttca
      601 cccgcgggcc gatcgcggtg catgccttca tccgtgacca tcgccgctgc accgatagcg
      661 atccggtgcg cttccgcttc ggcaagccga cgcacctcta tgacatgcgg gccgggcgct
      721 acctgggcca tgtggcggag acagaagcgg tcgtcccgcc cggccagacc gccctctatg
      781 cctgccttcc ctatcaggta acgaaggtca cggtcacgct cccatccaag gtcagagggg
      841 gctccgtgct caccgcgcgt gcgagcatca gcgcgggcga ggcgagaccc ggggaccaca
      901 tcttccacat ccaggtgatc gaccctgccg ggaacgaggt acgtgcctac accacaaacg
      961 ccctagcgcc cggtggccag ctggatctac cactgtcctt cgcctggaat gatccgccag
     1021 gcgcctggga ggtggtcgtt cgcgacgtgc tgaccggcgt ctccggggcg gctgggttca
     1081 cgctggaatg acgaggcgac gcccgcgctg cttggctggc gcgggccaaa agaccggcgc
     1141 tgcgtaactc gcgcaggcgt agcagggcat ccggaagcgg cgtcgtatga gagaataacg
     1201 agcggctgtg aatgccgccg caggagggca gcaatgaaga aaggttttgt tttcagttgc
     1261 atcccgacca aacgggggaa cgggaccccg atgaccccag aggaccggct tcgcctggcg
     1321 aaggatgccg gtttccaggg cgtcgagatc agcaccatgg atgacctggg tgagtgcgag
     1381 cgcctcgcca gccaggcccg gagcatcggg atcgagatcc acagcgtcat ggcgagcggg
     1441 cattggagct tcccgctctc cagccccgat gaggagacgc gccagaaagg cgtcgccaac
     1501 atccggcagt cggtagatac ggcgaaggtc tccggcgcgg ataccgtcct cgtcgtgccg
     1561 gccgtggtca acgagcagac cccgtacgag ctggcgtggg acctcgcgct gaagagcatg
     1621 cgcgagctgg cgccctacgc ggagaaaaac gggatctgcc tggcggtgga gaacgtctgg
     1681 aataagttcc tcctcagccc gcccgagatg ctccggttca tcgccgcctg tgagagcccg
     1741 gctgtcgccg cctacttcga cgtcggcaac atcctgatct atggctatcc cgaccagtgg
     1801 attcgcagcc tcggctcgac actgcgcaag gtgcacttca aggactttga tattcagacg
     1861 cgcaccttca ggaacctgct tcaggggaac gtgccctggg atagggtccg gcaggcgctc
     1921 ctggatgtcg gctacaccga ctactgcagc gttgagctgc cggcctacca gtcctacccc
     1981 gaccagttca tctacgactg ctcgaagcag gtggatcgca tcttcgctgg cgagtannnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacca gttcatctac gactgctcga
     2161 agcaggtgga tcgcatcttc gctggcgagt agccaacccg tgagagatag gagggggggc
     2221 cagcccccct cttgcattta tgcggcaggt gaatcgcgtc ttcgctggcg agtagcccgc
     2281 gaaagagtca gaggggggct atgcccccct ctcgttttct cagggtgtta cgccttcttg
     2341 ccatccaggg tggcaggaag ctcgccgtcc tgcgcgcccg cgactggcgt caggggcgtc
     2401 attttctgcg cgggcttggc cgcggcagcc gcaggcgtgg gtgcaggagc cgcgggcttg
     2461 ggcgcagagg gcgcgggttc tgccacggga gccggggtcg gggcctgagt ctccgcgcgc
     2521 gcgggcttgc cgtcaccatc agcgggcttg gtctgcagcg cgcgcccgag tatggagcgg
     2581 agatccagtc cggttacctc gcccagctgc tccatgaagc tgaaggcgat tgcgggcacg
     2641 ttgccggcaa acttctgcat gttcgtcgcg ccgttgttgc ccgcgtcgat catcacaacc
     2701 ttatcgatgc ccgcgagcgg gttgcccaca gcctggagcg gcccggacat ggcggagatg
     2761 atctccggca tcttgtcgac caccatctgc agcatagcgg cctcaccata ctgcttccat
     2821 gcctccgcct tctgccgtag accctcggcc tccgccagca acttgaggcg cgcagcctcg
     2881 gcctccgcct ttccctttgc cagcgcgacc gctgcctcgg cctcacccgt ctggcggatg
     2941 cgctcggcct cggcggcacc cttggcctgg atgacgctcg cctcggcgtt accgacctgc
     3001 tccacctgag ccgcctggcc tgcggcggcc gtctccagct ccttgcgctg ggcttcagca
     3061 cgcgcgatgc gtgcctcgcg ctcgccttct gctagggcga cctgggctct ctggtcgccc
     3121 tccgccttga tcacggccgc gcgctgcgca ccctcggcgg tgatgatcgc ggcctggcga
     3181 tccgcttccg ctttgataac cgccgcctgg cgatccgcct ccgccggctt caggacgctg
     3241 gcgaccagct ccttctcctt gcgcagcgct tcctgctcct gaacagcgat ctgcgcctgc
     3301 gtgcgcacct gctcgatgcg cacctcttcc atcaccaccg cctgccgcgc ctgggcctcg
     3361 gcaagggggc cctgctgact cgccttcgcg cgctcggact ccacctgcgc catgtactgc
     3421 gcgcgctgaa cctccaggtc acgctgagcc gctgcgatct tcgcctcggc ctctgccttg
     3481 gcgatctctc ccgcctggcg cgcggacgat gcctgaatgg tggcatcgcg attagctttc
     3541 gcctggccga tctctgcgtc gcgctccgcc tcggcacgcc cgatgctcgc atcgcgcttg
     3601 acctctgcgg tgcgccgctg cccgagcgag tcgaggtagc cttggtcgtc cgagatctgc
     3661 tggatggtga ggacatcgat tccgacgccc atcttcttca ggtcgacggc ggcctcatcc
     3721 atcattcggt tggcaaacgc ctgccggttg gcattgatgt cctcgaccgt gagcgtgccc
     3781 aggatcgcgc gcaggtggcc ttccaacgtc tggaagatca cgttttgcac ctgctggagc
     3841 gacatcccc
//
LOCUS       DAOSCV010000385         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15006_length_3842_cov_2.852654, whole genome shotgun sequence.
ACCESSION   DAOSCV010000385 DAOSCV010000000
VERSION     DAOSCV010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15006_length_3842_cov_2.852654"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..158)
                     /locus_tag="PLY56_10190"
     CDS             complement(<1..158)
                     /locus_tag="PLY56_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ21894.1"
                     /translation="MSNRFLFTSESVTEGHPDKMADQISDAVLDAILAQDPYGRVACE
                     TMLTTGLV"
     gene            complement(320..955)
                     /gene="gmk"
                     /locus_tag="PLY56_10195"
     CDS             complement(320..955)
                     /gene="gmk"
                     /locus_tag="PLY56_10195"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ21895.1"
                     /translation="MSRRGTVFVLSAPSGAGKSTVTQGVLERLPRVRRCITVTTRAPA
                     AGEVDAVDYFFRTPEEFQHLERQGELLESACVHGHYYGTPRWWVEDQIAQGTDILLVI
                     DVQGARSVRAQLERVVSIFLAPPSLPELERRLRTRARDPEAAIRTRLANAVTELQAIP
                     EYDYLVINDDLECAVAEIVAIITAETLRIPPGPAAQEMVKSLLRGDDAPGE"
     gene            1379..2347
                     /gene="corA"
                     /locus_tag="PLY56_10200"
     CDS             1379..2347
                     /gene="corA"
                     /locus_tag="PLY56_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOJ21896.1"
                     /translation="MTRLLVHHARHGFEPNVSVERLASLLSDPEILLWLDLERPTQEE
                     IALLHQVFRFHPLAVEDVIHAFQRPKIEKYENYFFIVLYAVSPHDASEELNPKELALF
                     VGPNYLVTVHPDAIPVIGEVTALWQASDQTSAHNVTWLLYLLLDAIVDDYFPVVDSLA
                     DRTDALEDRIFAEEETAALQGIFELKRVLLALRRVVAPERDVVNVLMRREHPFIDVRY
                     LAYLQDVYDHLSRLTESIDVFRDILASALDAHLSVVSNRLNQVMKTLTALSAMLMTSG
                     LVAAIYGMNFRRMPELEWTYGYPFALLLMLGIVVALGLFFHRKGWL"
     gene            2389..3567
                     /locus_tag="PLY56_10205"
     CDS             2389..3567
                     /locus_tag="PLY56_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ21897.1"
                     /translation="MEPEITLSPEVQEVLADVIAIRREIHAHPELGYQEVRTAALVAE
                     RLQAMGLSVQTGVAETGVVGLLEGGQPGPVVMLRADMDALPIQELTGYPYASQVPGVM
                     HACGHDGHTAALLGAAQVLSAMREQLAGSVKFVFQPAEESRAGAERMVQEGVLQAPRV
                     DAAFGLHLWNSMSLGTVGVTTGPAMAATDYFEIIIRGRGGHAASPHQAVDPVLAAAHF
                     LVTVQSIISRSRDPLAPAVFSIGCIRGGDAGNVIPDEVLLRGTLRTLSQENRTLLLKR
                     IEEVLHGTTQALGATYDYDHRPGYPVLVNDAAMADLAREVAAAVVGKEGVLSPEPTMG
                     GEDMAYYLQEVPGCYLWVGSANPSEEAYPHHNARFNMDERAIGIAVEILVRLARRYLA
                     "
BASE COUNT          662 a         1281 c         1214 g          685 t
ORIGIN      
        1 atcaccaggc cggtcgtcag catcgtctcg caggcgacac ggccataggg gtcctgggcc
       61 agaatagcat ccaacaccgc atccgagatc tgatccgcca tcttatcggg atggccctcg
      121 gttacggatt ctgaggtaaa gagaaacctg ttgctcaagt cgtcctccct ggagtcacag
      181 cgcgcgacgc gctcccactc actccccttg ccgtatgtgc gatcgaccgc ggtctcgcga
      241 tgcttttccg cggcggccgg ccccaccgcg cgggcgggca gtcatggcgc ggtgagccga
      301 ggccagacac tgcggtgcat cattctcctg gggcatcgtc tccgcggagg agcgacttca
      361 ccatctcctg ggcggccgga ccagggggga tgcgcagggt ctccgcggtg atgattgcaa
      421 caatctcggc gacggcgcac tcgaggtcgt cgttgatcac caggtagtcg tattctggaa
      481 tcgcctggag ctcggtgacc gcattggcca ggcgcgtcct gattgcggcc tccgggtctc
      541 tggctcgcgt ccgcaggcgg cgctccagct ctggcagcga tggcggcgca aggaagatgc
      601 tgaccactcg ctctagctgc gcccgcaccg agcgcgcgcc ctgaacatcg atcaccaaca
      661 ggatatcggt gccttgggcg atctgatcct cgacccacca tcgcggcgtg ccatagtagt
      721 ggccgtgcac gcaggcggac tccagcagct cgccctgcct ctcgagatgc tggaactcct
      781 cgggagtgcg aaagaagtaa tcgacggcat ccacctcacc tgcagccggg gcacgcgtgg
      841 tgacggtaat gcagcgccgt acccggggca atcgctcgag gacgccctgc gtgaccgtgc
      901 tcttccccgc tccagaaggc gcggatagta cgaacacggt tccccgacgg ctcactacgg
      961 tctcctcctc ctcgcgatct cgcctcctga tcggcgcgac ctgcccttca tccgggcgta
     1021 ttctagcata atccaggtca acggtcaacc aaccagacgc aatcatgttg ggggccaccc
     1081 gcgcgagcac cgcccggccg catcgccagc ccgcgcaccc acaattctgg cgccccggcc
     1141 agaacgccgg tctgcttcgc gctcctccga gagcacctca cccggacgcg atccacaccc
     1201 ccgggcaccg cgcgagctgg gccgccgcgc tattcccatc cgcgtccggc ccctgcatcg
     1261 acggtatcac agtcggcggg accggcccct tgacgctcgc cagtggccgc tctataatca
     1321 ggatggaatc cggcgcgccg cctcgttgcc gcgtcgcggc ggctccgagg agaggatctt
     1381 gaccaggcta cttgtccatc acgccaggca cggcttcgag ccgaacgtgt ccgtggagag
     1441 gctcgccagc cttctctcgg acccggagat cctgctgtgg ctggacctgg agcgccccac
     1501 gcaggaggag atcgcccttc tccaccaggt gttccgattc caccccctcg cggtggagga
     1561 cgtgatccac gcgttccaga gaccgaagat cgagaagtac gaaaactact tcttcatcgt
     1621 gctctacgcc gtctctcccc atgacgccag tgaggagctg aaccctaaag agctggccct
     1681 ctttgtcggc cccaactacc tggtcaccgt tcacccggat gcaattccgg tcatcggaga
     1741 agtcacggcg ctgtggcagg ccagcgatca gacctcggct cacaacgtta cgtggctcct
     1801 ctacctcctg ctcgatgcca tcgtcgacga ctatttcccc gtggtggact cgctcgccga
     1861 ccgcaccgat gcgttggagg atcggatctt cgcagaggaa gagacggctg ccctgcaggg
     1921 gatcttcgag ttgaagcgcg tgctcctggc gttgcgccgc gtcgtcgccc cggagcgcga
     1981 cgtcgtcaac gtgctgatgc gccgcgagca tcccttcatc gacgtgcgtt atctggcgta
     2041 cctacaagat gtgtatgacc acctgtcccg gctcacggag agcatcgacg tctttcgaga
     2101 tatcctcgcg agtgcgctcg acgcgcacct ctcggtggtc tccaaccggc tgaaccaggt
     2161 gatgaagacg ctcacagccc tctcggcgat gctaatgaca tccgggctcg tggcggctat
     2221 ctatgggatg aatttccggc ggatgccgga gttagaatgg acctacggct atccctttgc
     2281 gctcctgctg atgctgggga tcgtggtggc actgggcctc tttttccatc gcaaggggtg
     2341 gctgtagccg tagaggagaa gaaaatggga gatgggaaag gagcgatcgt ggagcctgaa
     2401 atcacgctca gtccggaagt tcaggaggtc ctggccgatg ttatcgccat acgccgtgag
     2461 atccacgccc acccggagct aggctatcag gaggtgcgca ccgcggcgct ggttgccgag
     2521 cgcctgcagg caatggggtt gtcagtgcaa accggggtgg cggagacggg ggtggtcggc
     2581 ctcttggagg gcggccagcc aggtccggtc gtcatgctcc gcgccgacat ggatgcgctc
     2641 cccatccagg agctgaccgg atacccctac gcgtctcagg tgcctggggt gatgcacgcc
     2701 tgcggtcatg atgggcacac ggcggcgctt ctgggcgcgg cgcaagtgct ctccgcgatg
     2761 cgcgagcagt tggctggcag cgtgaagttc gttttccagc cggccgagga atctcgcgca
     2821 ggggccgagc gcatggtgca agagggagtc ctgcaggcgc cgcgcgtgga tgccgcgttc
     2881 ggactccacc tctggaactc gatgtcgctg ggtacggtcg gcgtcaccac cgggccagcc
     2941 atggccgcga ccgattactt cgagatcatc attcgaggca gaggcggtca tgctgcctcg
     3001 ccccaccagg cggtcgatcc ggtcctcgcc gcggcccact tcctggtcac cgtgcagagt
     3061 atcatcagcc gctccagaga tcccctggcc cccgccgtct tctccatcgg gtgcattcgc
     3121 gggggtgatg cgggcaacgt gatcccggac gaggtgttgc tccggggcac tctgcgcacc
     3181 ctcagccagg agaaccggac tctgctcctg aagcgaatcg aggaggtgct tcacgggacc
     3241 actcaggcgc tcggggccac ctacgactac gatcaccggc caggctatcc cgttctggtc
     3301 aatgatgcgg cgatggcgga cctggcgcgc gaggtggcag ccgcggtcgt gggcaaggag
     3361 ggtgtgctct ccccggagcc aacgatgggc ggcgaagata tggcctacta cctgcaggaa
     3421 gtgcccggct gctacctgtg ggttggctct gcaaacccct cggaagaggc gtacccccac
     3481 cacaacgcgc gcttcaacat ggacgaacgc gccatcggga tcgcggtgga gatcctggtg
     3541 cgtcttgccc ggcgctacct ggcatagaga aacaggggta tgaaatccag aggcagtcgc
     3601 ctttctgtga aagacgcgct ctcctcacag cggctccgcg aagagcaagg gggctcatgt
     3661 cgctccccgg accctccggg ccgctgagga gaagccgccg cagcaacgct taggtggccg
     3721 ccaggcgccg gcggcgcgcg tactccagga gacggcgagg gctgatcacg ccgcggaact
     3781 cgccgtcatc aaccaccgcc agccactcca ggttctggtc ggacatacgc accagcgtct
     3841 cc
//
LOCUS       DAOSCV010000386         3835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15058_length_3835_cov_2.900529, whole genome shotgun sequence.
ACCESSION   DAOSCV010000386 DAOSCV010000000
VERSION     DAOSCV010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15058_length_3835_cov_2.900529"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PLY56_10210"
     CDS             <1..289
                     /locus_tag="PLY56_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ21898.1"
                     /translation="RESIVIYDEYGPVRMVRTREWKYVYRHAYGPHELYDLVNDPDER
                     VNRVEDPAMAARIRELKGLMEEWFTRYANPCRDGLREDGAAMGQTRFLPPA"
     gene            323..2776
                     /locus_tag="PLY56_10215"
     CDS             323..2776
                     /locus_tag="PLY56_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOJ21899.1"
                     /translation="MSPWLLLALVAAAGAPEQPLVWSFDDPQTSWRPRAAGITLEHAP
                     GVSAAPEGKGSLRIHGRIGDGWNYALSETHPMSAGRYYKLSARVRVDRLVPHAPAPFL
                     KCEFVAEDGRSLGRATTESYQTARMGQWQRVEGEFEAPKDTAKCWVALEKGTEGAMEI
                     DAYLDEVRIEPISTLQVFERYRLKPLPAPLHARRGVHPRLFLDTARVEQLRQAIGHTH
                     ADVWQEVRALADRAVRSGPPPYREHDSYSGDEQLWQREVGNTMPTLAIAYLLTKEVKY
                     LDAARAWALASCSYPTWGLGRLDGMDLATGHQLLGLALVYDWCYQDLGEDARRTIRET
                     LARRAGKMFEAAATGRVGWRRAYLQNHLWVNICGMQAAGLALYDELDDAPLWAGLALE
                     KLRLTIEALGPDGASHEGAAYWQYGAEYLLKLMHLSRELLAADFYDHPWWKNTAAYAL
                     YLSLPRQVWTRRNCIVDIADCPRGNWYGPDYILRGLAREYRDGHAQWLAAEVDAANID
                     APGARWLNLIWLDPEVPPLPPTNLPTLRHFEDMGIVSVRTDWSGNEALLVFKCGPFIG
                     HHAVEAFDYDPGGGHVHPDANHFVLFGAGEWLIRDDGYLPKATEHHNTLLVDGKGQLG
                     EGKMWFAGSEALAVKSRPRILQARSTAAVDHLVGDATEAYPKESGLRRYVRHLFFLKP
                     DVLIVADEIVTDQPRTLELRFHPERPAARGEDGAFLARGEKSALRIEVLTPEGAEVTA
                     EELPARSQGHRQGGLLPTIRVRAERQEWRNAVALSWSDAGSIPVRVTMKTEGDRWIFA
                     TKGRTITLDWREGRGDIAR"
     gene            complement(2803..>3835)
                     /locus_tag="PLY56_10220"
     CDS             complement(2803..>3835)
                     /locus_tag="PLY56_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21900.1"
                     /translation="ALGIDGDARRFNREAGLLRVAIEKHLRNPDNGLYYLNIDVDGRA
                     HSEVTADLVFPLIGGVSDEETTRLISERLNHPGFMTEAGLRTLSHFDPLYTPDRLVGL
                     QGGVWPGVTWWYAMSCLHADPGLMVEVLRSSYWQYIREPKRYNTVPGQFSEWFDGEAL
                     QNRGMRLSPWEPPRFLWALLEGALGVHPHYDRLAVEPTLPHDWKWCRVRNLPYRGQSL
                     SWFLARYGDGLHLLTTDPVETPLIMERFDEDVSDLVIPEGGNISVAAFAGSGRILLCL
                     GSTSAAKQPHLIALRALLENVRRYEVTLYSSEVDRWTRLGSYLGGALERLSIDVEGGG
                     FAILLLEAR"
BASE COUNT          648 a         1228 c         1342 g          617 t
ORIGIN      
        1 gcgcgagagt atcgtcatct acgatgagta tggcccggtg cgcatggtgc gaacccgcga
       61 gtggaagtac gtctaccgcc atgcctacgg tccccacgag ctctacgacc tggtgaacga
      121 cccggacgag cgcgtgaacc gggtggagga tccggcgatg gcggcgcgca tccgggagtt
      181 gaaaggcctg atggaggagt ggttcactcg ctacgccaat ccgtgccgcg atggtttgcg
      241 agaggatggc gcggcgatgg gacagacgcg cttcctgccg ccggcatgag agtgcccggc
      301 ggcccgtgat gggagggatc ccatgagccc atggcttctg ctggcattgg tggctgccgc
      361 cggagcgcca gagcagccgc tcgtttggag ctttgacgac ccacagacct cctggcgccc
      421 gcgtgccgcg ggcattaccc tcgagcacgc gccgggcgta tcggcggccc ccgaggggaa
      481 ggggagcctc cgcatccacg gtcggatcgg ggatggctgg aactatgccc tatcggagac
      541 gcatccgatg tcggctggcc ggtattataa gctctcggcc cgggtgcggg tggatcggct
      601 ggtgccccac gcgcctgcgc ctttcctgaa gtgtgagttc gtcgcggagg acgggcgctc
      661 gctggggcgc gcaacgacgg agagctacca gacggcacgg atgggacaat ggcagcgcgt
      721 ggaaggagag ttcgaggcgc cgaaggacac ggcaaagtgc tgggtggctc tagagaaggg
      781 cacggagggc gcgatggaga tcgatgccta tctagatgag gtgcgcatcg agccgatctc
      841 cacgctacag gtgtttgagc ggtatcggct caagccgctg ccagcgccgc tgcacgcgcg
      901 ccgcggcgtc cacccgcgcc tctttctcga taccgcgcgc gtggagcagt tgcgccaggc
      961 gattggccat acccacgcgg atgtctggca ggaggtccgg gcgctcgccg accgggcggt
     1021 tcgatcagga ccgccaccct atcgggagca cgatagttac agcggcgatg agcagctgtg
     1081 gcagcgcgaa gtgggcaaca cgatgcccac cctggcgatt gcctacctcc tcaccaaaga
     1141 ggtgaagtac ctcgacgcgg cgcgcgcgtg ggcgctggcg tcgtgctcct atccaacctg
     1201 gggtctgggc cgcctggatg gcatggacct ggccaccggc caccagctcc tgggactggc
     1261 cctcgtctat gactggtgtt accaggattt gggcgaggat gcgcgccgca ccatccggga
     1321 gacactggct cgccgggccg gcaagatgtt cgaggccgcc gcgacaggca gggtcgggtg
     1381 gcggcgcgcc tatctgcaga accacctctg ggtgaatatc tgcgggatgc aggccgcagg
     1441 gcttgccctc tacgacgagc tggacgacgc ccccctctgg gcggggcttg ccctggagaa
     1501 gctgcgcctc accatcgagg cgctcggccc ggacggcgcc agccacgagg gcgcggccta
     1561 ctggcagtac ggcgcggagt acctgctgaa gctgatgcac ctctcccgag agctgctggc
     1621 tgccgacttc tatgaccatc cctggtggaa gaataccgcc gcgtacgcgc tctatctctc
     1681 gctgccgcgc caggtctgga cccgcaggaa ctgcatcgtc gacatcgccg attgcccccg
     1741 cggcaactgg tatggccccg actacattct gcgcgggttg gcgcgcgagt accgcgacgg
     1801 gcacgcgcag tggctcgccg ccgaggtgga cgcggccaat atcgatgcgc ccggcgcgcg
     1861 ctggctcaac ctgatctggc tcgatcccga ggtgccaccg ctcccgccca cgaatctgcc
     1921 gacgctgcgt cacttcgagg atatgggcat cgtctccgtg cgcaccgatt ggagcggcaa
     1981 cgaggcgctc ctcgtcttca agtgcgggcc gttcatcggg caccacgcgg ttgaagcgtt
     2041 tgactatgat ccagggggtg gccatgtcca tcccgatgcc aaccacttcg tgctcttcgg
     2101 cgccggcgag tggctgatcc gcgacgatgg ctacctccca aaggcgaccg agcaccacaa
     2161 caccctcctg gtagatggca aaggccagct gggcgagggg aagatgtggt tcgcgggaag
     2221 cgaggccctg gcggtcaaat cgcgcccgcg catcctccag gcccgctcta ctgcggcggt
     2281 ggaccatctc gtgggcgatg cgaccgaggc ctatcccaag gagagcggcc tccgccgcta
     2341 cgtgcgccac ctcttcttct tgaagccgga tgtgctgatc gtcgccgatg agatcgtgac
     2401 cgaccagccg cgcacgttgg agctccggtt tcacccggag cggccggcgg cgcgcgggga
     2461 ggatggcgct ttcctcgccc gcggcgagaa gagcgcgctg cgcatcgagg tgctgacacc
     2521 cgagggcgct gaagtgacgg cggaggagct gccagcccgg agccagggcc atcgccaggg
     2581 cgggttgttg cccaccatcc gcgtgcgcgc cgagaggcag gagtggcgaa acgcggtggc
     2641 gctttcttgg tcggacgccg gttccatacc ggtgcgcgtg acgatgaaga cggagggcga
     2701 ccgctggatc ttcgccacga aggggcgcac catcacgctc gactggcgag agggaagggg
     2761 ggacattgcg cgctgaggcg gcgtgaagcg atccgcccag ctctaccggg cttcgaggag
     2821 gaggatggca aagccgccgc cctccacatc gatggagagg cgttccaggg cgccacccag
     2881 ataggagccc aggcgcgtcc accggtccac ctcgctactg tagagcgtga cctcgtagcg
     2941 ccgcacattc tccaggagcg cgcgcagggc gatcagatgc ggttgttttg ctgcgctggt
     3001 gctgcccagg cagaggagga tccgcccgga accagcaaac gcggcgacgc tgatgttgcc
     3061 cccctcgggg atgacgaggt cgctcacgtc ctcgtcgaat cgctccatga tgagcggcgt
     3121 ctcgacggga tcggtggtga ggaggtgcag gccatcgccg tagcgcgcca ggaaccacga
     3181 gagcgactgc ccccggtaag gaaggttgcg cacccgacac catttccagt cgtgaggtag
     3241 cgttggctcg accgccaatc ggtcgtaatg gggatgcacg cccagtgcgc cctccagcag
     3301 cgcccagagg aagcgcggcg gctcccaggg agagaggcgc atcccccggt tttgcagcgc
     3361 ctcgccatcg aaccactcgg agaactggcc gggcacggtg ttgtagcgct tcggctcgcg
     3421 gatatactgc cagtagctgc tccgcaacac ctcgaccatc agtccgggat cggcgtggag
     3481 gcaggacatg gcgtaccacc aggtgacgcc tggccacacg ccgccttgca gccccaccag
     3541 gcgatcgggg gtgtagagcg gatcgaagtg tgagagcgtg cgcaggcccg cctcggtcat
     3601 gaagccgggg tggttcagcc gctcgctgat cagccgcgtc gtctcctcat ccgacacgcc
     3661 accgatcagg ggaaatacca ggtcggcggt gacctcggag tgcgcccggc catcgacatc
     3721 gatattcagg tagtagagac cattgtccgg gtttctcagg tgtttctcga tggcgacgcg
     3781 gaggaggccg gcttcccggt tgaaccgccg ggcatcgccg tcgattccga gggcc
//
LOCUS       DAOSCV010000387         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15064_length_3834_cov_3.153215, whole genome shotgun sequence.
ACCESSION   DAOSCV010000387 DAOSCV010000000
VERSION     DAOSCV010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15064_length_3834_cov_3.153215"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLY56_10225"
     CDS             complement(<1..418)
                     /locus_tag="PLY56_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ21901.1"
                     /translation="MPANLTPAYREAEERFRQATTPEEKIECLEEMLAVIPKHKGTEK
                     LQADIKRRLSKLRQETMSGRGPARRETAFRVEKEGAGQIALVGPPNSGKSSLLAALTN
                     AQPAIGDYPFTTQAPTPGMMAYENVQIQLVDLPPVTT"
     gene            571..1971
                     /locus_tag="PLY56_10230"
     CDS             571..1971
                     /locus_tag="PLY56_10230"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOJ21902.1"
                     /translation="MITQQDLKEIERYVYEIPREFRADMRVPARVYASPELMKKIFSD
                     RSLEQLINTATLPGVEKYVIAMPDIHQGYGCPVGGVVATRADDGIISPGVTGYDINCG
                     VRLLATTTEARAVRKRISTLVDLLFTHIPSGVGGRGPIHLSEGDMAAVLEQGAAWAVK
                     RGYGAKEDLARIEEGGSARDAQAEAITDKARQRGRDELGTLGSGNHFVEVQRVERILN
                     PDEAERMGVFEDQLCFMIHSGSRGLGHQTCTDYVNICRRAMPQYGIQIPDQELACVPY
                     QSPEGQAYYHAMRAATNFAFANRQVLSHSVREAWDRAMAGVVKDREVRTIWEVGHNVA
                     TLFQHEGKELVVHRKGAARTEPGKPLLIPGSMGSASYLLVGTEKAERETFGTCAHGAG
                     RVMSRAQAKKRVQGQELRQQLEEQGVLARTKSYSGLAEEAPFAYKDVDAVIRVVEATG
                     LARPVARLVPLAVVKG"
     gene            complement(1978..3471)
                     /locus_tag="PLY56_10235"
     CDS             complement(1978..3471)
                     /locus_tag="PLY56_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOJ21903.1"
                     /translation="MRGQQAHWLARPRTRSREKAGKKMICTQCGKENPEDARFCAYCG
                     GIPAARSVPPSPAPRASRLPIIAAAVAVVLAGALAVAAAILWRVPRSLLTGRSPERST
                     QAAAVRPGDVPGAPGSLPPLPGSWDRGNPPAGARDVQGASPGAPMPGVTASHPSASPG
                     PPPGQVAANAPAGGITTGVPRSAPGTAVTGAQVPGSQGPGGAAVTAGTPGSLPPGASV
                     TAGTPGNVPSGRSVAAGTPGNQPSAPAVTAHPPSSAPSAPPVTGGRSGEVPPAPSLHA
                     GQPGNLPAGRSVTGREPGETPKSPSVTSSNGGQVPTGPSVQGNRPPASTPPRATAPRG
                     PDPRERNLVAEYLRRLELIQARDNQLNQQIGDLGGGWLGDAYTLGGLQDPAEFILRYA
                     RITQGMGQVAAAKQNLGNQFLNSCGPIARACPPVQNLHNWYIRYFQEWVQATQTLHGA
                     LRTRNLGLAMQVESQLSRVRNTKHQCDHEEDRVRDRYDIPHSPFGPR"
     gene            complement(3493..>3834)
                     /locus_tag="PLY56_10240"
     CDS             complement(3493..>3834)
                     /locus_tag="PLY56_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21904.1"
                     /translation="DTGKVYIFSNGDISMVCNHSMGPVNLFLDLPVPASQDLGQLRAA
                     VDAVGERLEREFGESLLERPHVDGLTAITGAQMTVRIRYRAVAPYQERVQMRLREEIR
                     DEFAQREIKLV"
BASE COUNT          612 a         1218 c         1363 g          641 t
ORIGIN      
        1 cagtggttac gggtgggaga tcgaccagct ggatctgaac gttctcgtag gccatcatac
       61 ccggagtggg agcctgcgtg gtgaaggggt agtcgccaat ggccggctga gcattggtaa
      121 gcgcggcgag caggcttgat ttcccactgt ttggcggtcc aaccagcgcg atctgcccgg
      181 ctccttcctt ctcgacacgg aacgccgtct cgcgccgggc tggaccgcgg ccggacatgg
      241 tttcctggcg gagcttggag agccgtcgct tgatatccgc ctggagtttt tcggtgccct
      301 tgtgcttggg gatgacggca agcatctcct ccaggcactc gatcttctcc tcgggggtgg
      361 tggcctgccg gaagcgctcc tctgcctccc ggtatgcagg agtgagatta gccggcattg
      421 ggtcactcct ctccagctat cgatggcact ttcctctacg attccccgcc ccgacgcggc
      481 cttcctgcag gagcgtccgg cttactcgcc ggtgtcttgc gcgatagggg tttacccggt
      541 ataatgggca gtgtggcctg gagggaggat atgatcacgc agcaggacct gaaagagatc
      601 gagcgctacg tctacgagat accccgggag tttcgcgccg atatgcgggt gccggcgcgc
      661 gtctacgcga gcccagagtt gatgaagaag attttcagcg acaggtcgct ggagcagctg
      721 atcaacacgg caacgctacc gggtgtggag aagtacgtca tcgccatgcc cgatatccat
      781 cagggctacg gctgtccggt gggtggagtg gtggcgacac gcgccgacga tggcatcatc
      841 tcgccggggg tcaccggcta cgacatcaac tgcggggtgc gccttctggc caccacgacg
      901 gaggcgaggg ccgtgcgaaa gcgcatctcg acgcttgtcg atctgctctt cacgcacatt
      961 ccgtccggcg tgggggggcg aggcccgatc cacctgagcg agggagacat ggccgccgtt
     1021 ctcgagcagg gcgctgcttg ggcagtgaag aggggctatg gcgcgaagga ggacctggca
     1081 cgaatcgaag aaggtggctc agcacgcgat gcgcaggcgg aggccatcac tgataaagcg
     1141 cgccagcgcg ggcgtgacga actgggcacg cttgggtcgg gaaaccactt cgtcgaagtg
     1201 cagcgcgtag agcggatcct gaatccagac gaagcagagc gcatgggggt gttcgaggac
     1261 cagctgtgct tcatgatcca cagcgggtcg cgcggcctcg gccaccagac gtgtaccgat
     1321 tacgtgaaca tctgccgccg ggccatgccc cagtatggga ttcagattcc cgaccaggag
     1381 ctggcgtgcg tgccgtatca atcgcccgag gggcaggcgt actaccacgc gatgcgcgcc
     1441 gccacgaact tcgcctttgc caaccgccag gtgctttccc actcggttcg cgaagcctgg
     1501 gaccgcgcca tggccggcgt tgtgaaggac cgtgaggtcc ggacgatctg ggaagtgggg
     1561 cataacgtcg ccacgctctt ccagcacgag ggtaaagagc tggtggtcca tcgcaagggc
     1621 gccgcgcgca cggagccggg gaagccgctc ctcatcccgg ggagcatggg cagcgcctct
     1681 tacctcctcg ttggcaccga gaaggccgag cgcgagacgt ttggcacgtg cgcccacggc
     1741 gccgggcgcg tgatgagccg cgcccaggca aagaagcggg tgcagggtca ggagctgcgc
     1801 cagcaactcg aggagcaggg ggttctggca cgcacgaaga gctactccgg cctggccgag
     1861 gaggcaccgt tcgcctataa ggatgtggat gcggtcatcc gcgtcgtgga agctactggc
     1921 ctcgcccgcc ccgtggcgcg cctggtgccc ctcgcggtgg tcaaagggta ggggctgcta
     1981 tcgcggaccg aaggggctgt gggggatgtc gtaacggtcg cgcacccggt cctcctcatg
     2041 gtcgcactgg tgcttggtgt ttcgcacgcg tgagagctgt gactcgacct gcatcgccag
     2101 gcccaggttg cgggttcgca gggcgccatg aagcgtctgc gtcgcctgca cccactcctg
     2161 gaagtagcgg atataccagt tgtgcaggtt ctgaactggc gggcaggcgc gcgcgatcgg
     2221 gccgcatgag ttcaggaatt ggttgccgag gttctgcttc gccgcagcca cctgacccat
     2281 gccctgggtg atgcgggcat atcggaggat gaactccgcc gggtcctgca agccccccaa
     2341 cgtgtacgcg tcgcccaacc agccgccgcc caggtccccg atctgctggt tgagctggtt
     2401 gtcgcgcgcc tggatgagct ccagccggcg cagatactcg gcgaccaggt tccgctctcg
     2461 cgggtccggc cctcgcggcg ccgtagcgcg cggtggcgtc gaggctggcg gccggttgcc
     2521 ctgcacggag gggccagtcg gaacctggcc cccgttgctc gatgtgacac tggggctttt
     2581 cggggtctct cccggctctc tgccggtgac agagcgcccg gcaggaagat tgcctggctg
     2641 acccgcgtga agcgagggcg cgggggggac ctcgccggat cggccgccgg tcaccggcgg
     2701 ggcgctcggg gcagaggagg gtggatgcgc ggtgactgcg ggcgccgatg gctgatttcc
     2761 cggagttcca gcagccacag atcggcctga tggcacgttg cccggcgtac ccgcggtcac
     2821 gctcgctcca gggggcaatg agccgggggt gcccgccgtc acggcggcgc ccccaggacc
     2881 ctggctgccg ggaacctgcg cgccagttac cgcggtgccc ggggcgctcc tgggaacccc
     2941 ggtggtgatc ccgcccgccg gagcgttcgc agcgacttgg cccggcgggg gccccggaga
     3001 cgcggacgga tggctggccg tcaccccagg catgggcgcg ccgggtgacg cgccttgaac
     3061 gtctcgcgcg ccggccggcg gattgccgcg atcccacgag ccgggaaggg ggggcagcga
     3121 tccgggcgcg ccagggacat cgcccgggcg taccgccgcg gcctgcgtgg aacgctctgg
     3181 ggaacgcccg gtcaggagtg agcggggtac tctccagagg atggcagcag ccacggcgag
     3241 cgcgcccgcg aggacgacag ctaccgcggc ggcgataatc ggcaggcgcg acgcgcgggg
     3301 tgccggagaa ggcggcacgg agcgcgcggc cgggatccct ccgcagtagg cgcagaagcg
     3361 cgcgtcctcg gggttctctt tgccgcactg cgtgcagatc atcttcttcc ctgccttctc
     3421 ccgcgagcgg gttcggggcc gggcaagcca atgcgcctgc tggccgcgca tcgctcctcc
     3481 ccggcgaaag ggctacacca gtttgatctc ccgctgggcg aactcatcgc ggatctcctc
     3541 gcgcaggcgc atctgtaccc gctcctgata gggggccacc gcccgatagc ggatgcgcac
     3601 tgtcatctgg gcgcctgtga tggcggtcaa tccatccaca tggggccgct ccaacaggct
     3661 ttcgccaaac tcgcgctcca ggcgctcgcc caccgcgtcg acggcggcgc gcaactggcc
     3721 gagatcctgt gacgccggaa ccggcagatc gaggaaaagg ttcaccgggc ccatcgaatg
     3781 gttgcagacc atcgatatgt cgccgttcga gaagatgtag accttccccg tgtc
//
LOCUS       DAOSCV010000388         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15110_length_3826_cov_3.015911, whole genome shotgun sequence.
ACCESSION   DAOSCV010000388 DAOSCV010000000
VERSION     DAOSCV010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15110_length_3826_cov_3.015911"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /gene="alaS"
                     /locus_tag="PLY56_10245"
     CDS             complement(<1..1770)
                     /gene="alaS"
                     /locus_tag="PLY56_10245"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ21905.1"
                     /translation="MTTDEIRELYLRFFETKGHLRLPSASLIPDDPTLLLTGAGMNPF
                     KAYFTGTETPPSRRVVTCQKCVRVGDVDNVGRTPRHHTFFEMLGNFSFGDYFKREAIQ
                     WAWEFSTEWLKLDPERIWVTIYLDDEEAYEAWTKDVGLPPHRVGRLGEHDNFWPADAP
                     TLGPNGPCGPCSELYYDLGEERGCGKPDCRPGCDCDRWLEYWNLVFQVYDRKDGGVLD
                     PLPQKNIDTGLGLERAAAILQGTTTNFETDTFRPIVARVEEMTGIRYGEEARTDVFMR
                     VIADHLRAAVFLVGDGVNPGNTGRGYILRRLARRAILRGDLLGLKEPFLHTMVPLVVS
                     LFQGAYPDLPGKETYIGRVLRTEEENFRRTLAQGMQRLEEYLASAEGTVIPGEVAFLL
                     YGTFGFPLELTQEIAAERGFTVDQEGFARSMEEEKRRARERAKMQGDIFVTEKGALGE
                     MIRTLPATEFLGYAVTEAGAQVLGIVFGEKALDEASEGQEVQIILDRTPFYAESGGQI
                     GDTGELRTPGGRVEVRDTIKKGDLFVHQGVVLAGVVRRGDSVTAVVEGSRRLAIRRNH
                     SATHLLHAALRQVLGPHVEQKGSL"
     gene            1998..2192
                     /gene="rpsU"
                     /locus_tag="PLY56_10250"
     CDS             1998..2192
                     /gene="rpsU"
                     /locus_tag="PLY56_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ21906.1"
                     /translation="MAQVTVKPNESLDSALRRFKKQIQEAGVLKEARRHEHYEKPSER
                     RRRAEAARRRKIKAAEEASR"
     gene            2625..>3826
                     /locus_tag="PLY56_10255"
     CDS             2625..>3826
                     /locus_tag="PLY56_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21907.1"
                     /translation="MRAWLRMVETHRLTLGLFTAIVLTAITALHLVPDKVSLEIGEIS
                     TKEVRAPRAARYVDTAATERLRRQYIATVAPQYEVDAYATATAEAAVNHAFSRLETVR
                     GQVAGQPLAERMHRVRAQLVDELGVPLTEESAEAALTVDKQTLDLLRGRARHLVLSTM
                     QHPEGIREGTEDLRRAADSIAEAAAELALPAPQRDLVRAIAQHALRPTLAFSAEKTER
                     ARRQVSAMVKPVIAEILPGEPVIHKGETVTQAHLDKFTALGLRQPRLDWRGGIYLALL
                     MTGIVGLIGAYLYRYHRPIYNDRRLLWLLALVVVGSLTAFKVGGSALGIKLDTTQYGY
                     FGVLCISTASMLISVLLHPALAVVVAALFSLHVGIMASFDLRFAGVALFSSLVGIHGF
                     SRIRDRGDV"
BASE COUNT          672 a         1236 c         1256 g          662 t
ORIGIN      
        1 cagcgagcct ttctgctcga cgtgcgggcc gagcacctgg cgcagggccg cgtggagcag
       61 gtgcgtggcg ctgtggttgc gccggatcgc caggcgccgg ctcccctcga ccacagctgt
      121 gaccgagtcg cccctgcgta ccacgccggc gaggaccacg ccctggtgca cgaacaggtc
      181 gcccttcttg atcgtgtcgc gcacctcgac gcgcccgcct ggtgtgcgca gctcaccggt
      241 gtcgccaatc tgcccgccgg actcggcgta gaacggggtg cgatccagga tgatctggac
      301 ctcctggccc tcgctcgctt catccagcgc cttttctccg aaaacgatcc cgagcacctg
      361 ggcgcctgcc tcggtgacgg cgtagccaag aaactcggtg gcagggagcg tgcgaatcat
      421 ctcgcccagg gcgcccttct cggtgacaaa gatatcgccc tgcatcttgg cgcgctctcg
      481 cgcccggcgc ttctcctctt ccatcgagcg ggcgaagccc tcctggtcga ccgtgaagcc
      541 gcgctccgcg gcgatctcct gcgtcagctc cagcgggaag ccaaaggtgc catagagcag
      601 gaacgccacc tcacccggaa tgaccgtgcc ctcggcgctg gccaggtact cttccaggcg
      661 ctgcatcccc tgggcgagcg tgcgccggaa gttctcctcc tcggtgcgca gcacgcgtcc
      721 gatgtaggtc tccttccctg gcaggtcggg ataggcgccc tggaagagcg agaccaccag
      781 aggcaccatc gtatgcagga acggctcctt caggccgagg aggtcgccgc gcaagatcgc
      841 ccggcgcgcg aggcggcgca ggatgtagcc gcgcccggta ttccccggat tgaccccatc
      901 gcccaccagg aagacggcgg cgcgcaggtg gtcggcgatg acgcgcataa agacatcggt
      961 gcgcgcctcc tcgccatagc ggatgcctgt catctcctcg acgcgggcaa cgatgggccg
     1021 gaaagtatcg gtctcaaagt tggtcgtggt gccctggaga atggcggccg cgcgctcgag
     1081 gccgaggccc gtatcgatgt ttttctgggg cagcggatcc agaacgccgc catccttgcg
     1141 gtcgtaaacc tgaaagacga ggttccagta ttcgagccag cggtcgcaat cacacccggg
     1201 ccggcaatcc ggcttgccgc agccgcgctc ctcgcccagg tcatagtaga gctcggaaca
     1261 ggggccgcag gggccgttcg ggccgagggt gggggcatcg gccggccaga agttgtcgtg
     1321 ctcgccgagg cggcccacac ggtggggtgg caggcccacg tccttcgtcc acgcttcata
     1381 ggcttcctca tcatccaggt agatggtcac ccagatgcgc tccgggtcga gcttcagcca
     1441 ctcggtgctg aactcccacg cccactggat cgcctcgcgc ttgaagtagt cgccaaagct
     1501 aaagttgccc agcatctcga agaaagtgtg atgccggggt gtacgcccga cattatccac
     1561 gtcgccgacg cgcacgcact tctggcaggt gacgacgcgc cgggatgggg gcgtctcggt
     1621 gccggtgaag taggctttga acgggttcat ccccgcgccg gtcaacagga gggtaggatc
     1681 atcgggaatg agcgacgcgc tcggcaagcg caggtgcccc ttcgtctcaa agaagcggag
     1741 gtaaagctcg cggatctcgt ctgttgtcat ggaacgcatg agtcattggc ctcgcaatac
     1801 atgtagaatc gctgggagaa tctttcacga tgcagggggt atcgtcaagg cgagagcgcg
     1861 cgttcggggg caagatccgc cttgacaagg cccactcgtt caggtataat caagcctagc
     1921 gatttttgcc atattggtta agagcgagat tggatgcact tcgacctcgt ctcggccagc
     1981 agaggggggg atgcgagatg gcgcaagtga ccgtgaagcc gaacgagtcg ctcgacagcg
     2041 ccctgcggcg gttcaagaag cagatccagg aagctggagt gctgaaggaa gcgcggcggc
     2101 atgagcacta cgagaagcca agcgagcgcc gccgccgggc cgaggctgcc cgccggcgca
     2161 agatcaaggc tgccgaagaa gcttcccgct gaagctggcg gcgacaataa tcaagctgta
     2221 agagtcgtta cctggtcaca accagagagt cgccgccccg gtctttccgg ggcgggcgcg
     2281 tctatgggat accccagggt tctgatgccg aagctcaagg cagagaggga acgcctcctg
     2341 ccttgggctc caaggcgaag actttggcgg gtggctagac gacctgacgg catcacccac
     2401 ccgtgatggc cctgatcctt tcctggagaa ctgccaggcc atccccattc cgagcggaac
     2461 ggcccgccga aaacggcgtg tcctgagtca gtgttcgtga ctcattgacc gctcgatgta
     2521 ggccattcca ggggtcacgg gtaggaggcc attgcacgtt gagatccgcc aagagcagtg
     2581 ggagcagggg ccacggcagg cgcgaaaaga gcctggcgcg cagcttgcgt gcttggctgc
     2641 gcatggtgga gacgcaccgg ctcacgcttg gcctctttac cgcaattgtg ctgaccgcga
     2701 tcacggccct ccatctggtg ccggataagg tctcgctcga gatcggcgag atcagcacga
     2761 aggaggtgcg cgcgccccgc gcggcgcggt acgtcgatac cgccgccacc gaacgcctcc
     2821 gtcgccagta tatcgccacg gttgctcccc aatacgaggt agatgcctac gcgacggcca
     2881 ccgccgaggc ggcggtcaac cacgctttct cccgcctgga aaccgtgcgc gggcaagtgg
     2941 ccgggcagcc actggccgag cgcatgcacc gagtccgcgc gcagctggtg gatgaactgg
     3001 gcgtgccgct gactgaagag agcgccgagg cagctctcac cgtcgataag cagaccctgg
     3061 acctcctgcg cgggcgggcg cgccatctgg tactctccac gatgcagcac ccggagggga
     3121 tccgcgaagg cacggaggac ctacgccggg cggcagattc cattgccgaa gcggcagccg
     3181 agctcgctct tccggctcct cagcgcgacc tggtgcgcgc cattgcacag cacgctcttc
     3241 gccctacgct tgcattcagc gccgagaaga ccgagcgcgc gcgcaggcag gtttctgcca
     3301 tggtgaagcc ggtgatcgcg gaaatcctcc cgggcgagcc ggtgatccat aagggcgaga
     3361 cggtcaccca ggcgcacctc gataagttca cggcgctcgg cttgcgccaa ccccgcctcg
     3421 attggcgcgg ggggatctac ctggcgctgc ttatgaccgg catcgtcggg ctgatcgggg
     3481 cgtatctgta tcgctatcac cggccgatct acaacgaccg ccggcttctg tggctattgg
     3541 cgctggtggt cgtcggcagc ctcaccgcct tcaaggtggg cggcagcgcg ctcgggatca
     3601 agctggatac cactcagtac ggctacttcg gcgtcctgtg cattagcacc gcgtcgatgc
     3661 tgatcagcgt ccttttgcat ccggcgctcg ctgtggtggt cgctgcgctt tttagcctgc
     3721 acgtcggcat catggccagt ttcgacctgc gcttcgcggg cgtggcgctg ttcagcagcc
     3781 tggtcggcat ccacggcttc tcgcgaatcc gcgaccgcgg cgatgt
//
LOCUS       DAOSCV010000389         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15112_length_3826_cov_2.944577, whole genome shotgun sequence.
ACCESSION   DAOSCV010000389 DAOSCV010000000
VERSION     DAOSCV010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_3826_cov_2.944577"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /gene="prs"
                     /locus_tag="PLY56_10260"
     CDS             <1..542
                     /gene="prs"
                     /locus_tag="PLY56_10260"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ21908.1"
                     /translation="KPVDHLYAMPVLAEDFRARAIPDLVVVSPDMGFAKRARHFALLL
                     DSPVAIGDKVRRGHEERAEVLSIVGDVRGCNALIVDDIIVSGTSLVSLAEVLVREGAK
                     EIYAAATHGVLSEGAVERLAASPIKEVVITDTLPLPPEKHHPKIRQVSVTPLFAEAIR
                     RIHEEESVSFLFQDHPPPP"
     gene            complement(546..1526)
                     /locus_tag="PLY56_10265"
     CDS             complement(546..1526)
                     /locus_tag="PLY56_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21909.1"
                     /translation="MNASAMPPLAVRPYQLLCIVCRIGAGRGDDLGDDRLAAILSAVR
                     DDPLRPVTLRCNADSCYRYQNPGRDEDTPEGALFNEKRDLDILQRMGLVPGDTRPAIE
                     VFERLLATVTETMGICAYPRETSPAWRGCAAARSSNYERGREMGLAAVIPGRPAEEMA
                     AEKEASARRIYEADRLLIRPHHLMCMTCFHGGREELAPIAADNLFEAIDVIHKRPEIP
                     ITLVRGCCMICPPCSLYDPGTGLCRAGMGMNLRDQKKDLDVLQKLGLEYGDTVPAREL
                     LELLYERIPSTRDVCGYGDGKERAWEWRICGEPEGREAYARGRAAKLGLS"
     gene            complement(1639..2802)
                     /locus_tag="PLY56_10270"
     CDS             complement(1639..2802)
                     /locus_tag="PLY56_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21910.1"
                     /translation="MDKLRIGSIGVSGRGALARHWHQPGPKGRSLVVAGADTNEQFLA
                     NFKEKVNPDAFVTTDYRKLLERDDIDAVAVTVPDFLHEEIACAALQAGKHVFCEKPMA
                     ITVEGCDRMLETARASGKKLFIGHNMRYMDIFRTMKEIVDAGVIGEIKAVWVRHFVGL
                     GGRFYFHDWHARRDRANTLLLQKGSHDIDMIHWITGRYSKRVAAFGSLDYFGGERPND
                     LICPECDEFKTCPDAQASWPGHEQCAYRREIDVEDNQVMIMQLEGGIKASYLQCHFTP
                     EYFRNYTFIGTEGRLENLDDSSKIIVKTRRRFGAKGLADRVYEVKPAEGGHGGADPVI
                     CEDFLDLVLLDKPTVATPLAGRMAVATGCAGAESMRQGGVVVDIPQPPAWVTE"
     gene            complement(3000..>3182)
                     /locus_tag="PLY56_10275"
     CDS             complement(3000..>3182)
                     /locus_tag="PLY56_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21911.1"
                     /translation="QRFVETNAALESATTLADARRALALAQETARLLERARESGAPPD
                     AASNSSSFSPSDNGQK"
     assembly_gap    3183..3192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3193..3809)
                     /locus_tag="PLY56_10280"
     CDS             complement(<3193..3809)
                     /locus_tag="PLY56_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21912.1"
                     /translation="MRGLQQEDVLVDAGVTRLTADDERALEAFAENGRPKLKILVLAE
                     EPAGGRASFTLNLHRYLSLGETALVVVTPEGVSAHSEALSREEMQRLTDDAAPEFALS
                     WRQGIEHLGNGIRAEVRARSVRARAALLAFVLAVALLLSWRALQRRRELERVLAEARD
                     RAAELAQAIHEAETDARLSQDPRVKEYYAQASQRFRQLGGRRFTSR"
BASE COUNT          607 a         1261 c         1289 g          659 t
ORIGIN      
        1 gcaagccggt ggatcacctc tacgcgatgc cggtgctggc ggaggatttc cgcgcgcgcg
       61 ccatcccgga tctggtcgtc gtatcccccg atatggggtt cgcgaagcgc gcgcggcact
      121 tcgcgctcct gctggactcg ccggtcgcta ttggcgacaa agtgcgccgc gggcacgagg
      181 agcgcgccga ggtgttgagc atcgtgggcg acgtccgggg gtgcaacgcc ttgatcgtgg
      241 atgatatcat cgtctcgggc acctcgctcg tgtccctggc ggaggtgctg gtgcgcgagg
      301 gcgcgaagga gatctatgcc gcggcaaccc acggggtgct ttcagagggc gcggtggaga
      361 ggctagccgc cagcccgatc aaggaagtgg tgatcaccga cacactccct cttccaccgg
      421 agaagcacca tcccaagatc cggcaggttt ccgtcacccc cctctttgcc gaggcgatcc
      481 ggcgcatcca cgaggaggaa tcggtcagct tcctctttca ggatcatccc ccgccgcctt
      541 agggcttagg agagcccaag cttcgccgcg cgccccctgg catatgcctc gcggccctcg
      601 ggctcgccgc agatccgcca ctcccaggcg cgctccttgc catccccata gccgcagacg
      661 tcgcgggtgg atgggatccg ctcgtagagg agctccagga gctcccgcgc cgggacggta
      721 tctccatact ccaacccgag cttctgtagc acatccaggt ccttcttctg atcgcgcagg
      781 ttcatcccca tgccggcgcg gcagagaccc gtgccggggt cgtagaggct gcagggcggg
      841 cagatcatgc agcacccgcg caccagggtg atgggaattt ccggtcgttt gtggatgaca
      901 tcaatcgcct caaagaggtt gtcggcggcg atgggcgcca gctcctcgcg tccgccgtgg
      961 aagcaggtca tgcacatcag gtggtggggg cggatcagga gccggtcggc ctcgtagatg
     1021 cgccgggcgg atgcctcttt ctcggccgcc atctcctcgg cgggccggcc ggggatgacg
     1081 gcggcgaggc ccatctcccg cccgcgctcg tagttgctac tccgggctgc ggcgcaaccc
     1141 ctccatgccg gtgatgtctc tcgcgggtag gcgcagatcc ccatcgtctc cgtcacggtg
     1201 gccaacaggc gctcgaacac ctcgatggca gggcgcgtgt cgccaggcac gaggcccatc
     1261 cgctggagga tatccaggtc gcgcttctcg ttgaaaagcg cgccttcggg ggtgtcctcg
     1321 tcgcgcccgg ggttttggta gcggtagcag ctatccgcat tgcagcgcag ggtcaccggg
     1381 cgcagcggat cgtcgcgcac ggcgctcagg atggcggcga gacggtcgtc tcccaggtca
     1441 tcgccacggc ccgcgccgat ccggcagacg atgcagagga gctgataggg cctgacagcg
     1501 agaggtggca tcgcagaggc attcacggtc actctcctct cggctctctg agggggcctg
     1561 gcagcgagga attgcaccgg gcatcccggt tctgaaacgc ggcagcgcgc gtttaggggc
     1621 gcgctgccgc agttcacgct actccgtcac ccacgcgggc ggctggggaa tatcgaccac
     1681 cacgccaccc tggcgcatcg attccgcgcc agcgcagccg gtcgccacgg ccatgcggcc
     1741 cgccagaggc gttgccacag tgggcttgtc gagcagaacc aggtcgagga agtcctcgca
     1801 gatcaccggg tcggcgccgc catgaccgcc ttcggccggt ttcacctcgt agacgcggtc
     1861 ggccagcccc ttggcgccga agcgccggcg ggtcttgacg atgatcttgc tggagtcgtc
     1921 cagattctcg aggcggccct cagtgccgat gaaggtgtag ttgcggaagt actcgggggt
     1981 gaagtggcac tgcaggtacg aggccttgat cccgccctcg agctgcatga tcatcacctg
     2041 gttgtcctcg acgtcgatct cgcggcggta ggcgcactgc tcgtggccgg gccaggaggc
     2101 ctgggcgtcg gggcaggtct tgaactcatc gcactcgggg caaatcaggt cgttcgggcg
     2161 ttcgccaccg aagtaatcga gcgagccgaa ggcggccacg cgcttcgagt agcgcccggt
     2221 gatccagtgg atcatgtcga tatcgtgcga gcccttctgc aggaggaggg tgttcgcccg
     2281 gtcgcggcgc gcgtgccaat catggaagta gaagcgcccg cccaggccga caaagtggcg
     2341 cacccacacc gccttgatct cgccgatcac gcccgcgtcg acgatctcct tcatggtacg
     2401 gaagatgtcc atgtagcgca tgttgtggcc gatgaagagc ttcttgcccg aggcgcgcgc
     2461 ggtctcgagc atgcggtcgc acccttccac cgtaatcgcc atcggcttct cacagaagac
     2521 gtgcttcccc gcctgcagcg cggcgcacgc gatctcctcg tggagaaagt cgggcacggt
     2581 gacagcaacg gcgtcgatat catcgcgctc cagcagcttc cggtagtcgg tggtaacgaa
     2641 ggcgtccgga ttcaccttct ccttgaagtt cgcgagaaac tgctcgttcg tgtcggcgcc
     2701 cgcaaccacc agtgaacgcc ccttgggacc gggctgatgc cagtggcgcg cgagcgcccc
     2761 gcgcccggac acgccgatgc tgccgatacg tagcttatcc atcgaaaact cctcctgtat
     2821 ttccaataga agaggcaggt tggctgtgcc gcctccagcc agcgctatct gacggcaccc
     2881 tcaaggtagg ttacgcaccc ggggtgcgtt tcccttgtca aatcggaaga atgcgcgccg
     2941 cggattgccc cacccgccgg accccagggg aaggggagcg accgggtggc ggcgccgcgt
     3001 catttctggc cgttgtcaga gggcgagaag gatgatgagt tgctcgcggc atccgggggc
     3061 gcgcccgact ccctcgcacg ctcgaggagg cgcgcggttt cctgcgccag cgcgagggcg
     3121 cggcgcgcgt ccgcgagagt ggtcgcgctc tccagcgcgg cgttggtctc cacgaagcgc
     3181 tgnnnnnnnn nncgcgaggt aaagcgcctg cccccgagct gccggaagcg ctggctcgcc
     3241 tgggcgtaat actccttgac gcgcgggtcc tgggagaggc gcgcatcggt ctccgcttcg
     3301 tggatggcct gggcgagttc ggcggcgcga tctctggcct cggcgaggac gcgctccagc
     3361 tccctgcggc gttggagcgc gcgccacgag aggaggaggg ccacggcgag gacgaatgcg
     3421 aggagggcgg cgcgagcgcg aacggaacgc gcacggacct ccgcacggat gccgttgccg
     3481 aggtgctcga tcccctggcg ccacgagagg gcgaactcgg gtgccgcgtc gtccgtgagg
     3541 cgctgcatct cctcccgcga gagcgcctct gaatgcgcgg agactccttc aggcgtcacc
     3601 accacgagcg cggtctctcc gagcgagaga tagcgatgca ggttgagggt gaaggatgct
     3661 cttcccccag cgggctcctc tgcaagcacc aggatcttca gcttcggacg cccattttcg
     3721 gcgaatgcct ccagcgcgcg ttcatcatcg gcggtaaggc gtgtgacgcc cgcgtctacc
     3781 agcacatcct cttgctgaag gccgcgcacg accgcctctt catcct
//
LOCUS       DAOSCV010000390         3812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15184_length_3812_cov_3.699494, whole genome shotgun sequence.
ACCESSION   DAOSCV010000390 DAOSCV010000000
VERSION     DAOSCV010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15184_length_3812_cov_3.699494"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1991)
                     /gene="uvrC"
                     /locus_tag="PLY56_10285"
     CDS             complement(99..1991)
                     /gene="uvrC"
                     /locus_tag="PLY56_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ21913.1"
                     /translation="MMESNEEAWHGEWDEMTLQEKLAILPDRPGSYLMKNAAGEILYV
                     GKAVSLRNRVRSYFHASADHTPKVRRLVSEIADIDWIITDTELEALILECNLIKKYRP
                     RFNVRLRDDKHYPYICVTLTEKYPRLMLVREAKKDGNRYFGPYTDARAVRQSIALLSR
                     IFRLRSCHHQVTGNEPEKLCLYYHINECSGPCAGKISQEEYRETVEEACLFLEGRQDA
                     LLKQLRQEMERSAERLNFERAARLRDQIKALETIRERQKVLSTALVDQDVVALVSDDG
                     QACAQLLFMRNGRLVGQEHFYLEGTTDEELSQATAEFLKQYYQDATYAPKEVLVERDI
                     AEHDIIEAFLRQKRGKKVHITAPSRGEKKRLVEMAHTNALDALRQLKVKLDSDKARNQ
                     EALTALKEALEMERVPLRIEAYDISHIQGVETVGAMVVLYAGAPRKSDYRRLIIKSLG
                     PNPDDFAAMKEVVGRRLARGKMGDEKFREMPDLLLIDGGKGQLNAALEAMREMEVEIP
                     EVGLAKQFEEIFLPGQPEALRLPRHSPALHLLQRVRDEAHRFAISYHRKRRSRRSTRS
                     LLDSVPGIGPKRKRELLRHFGSLARIRQATLEELAACPGMNQRAAEALYHSLCTAGTE
                     TAAADA"
     gene            2475..>3812
                     /locus_tag="PLY56_10290"
     CDS             2475..>3812
                     /locus_tag="PLY56_10290"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ21914.1"
                     /translation="MEHKVHIFDTTLRDGEQSPGASLTGREKLEIAHQLARLNVDVIE
                     AGFPCSSPGDFKAVQEIARSVKGPVITALARAVKADIDAVWESVREAERPRIHIVLGS
                     SDIHAKHKFRRSREELLAMGVEAVRYARTLCPDVEYSTEDASRSDRRYLYQVLEAVIE
                     AGATVVNIPDTVGYAVPAQWGRLIADIRRNVRNIDQAILSVHCHNDLGMAVANSLAAV
                     RAGAQQVECTINGIGERAGNASLEEVVMALKVRRNYYGAYTDVVTSELCRTSSLVSRL
                     TGLTVARNKAVVGANAFAHSSGIHQDGVLKNRDNYEIFSPDDVGASGTEIILTARSGR
                     HAVRHRLEGLGYTLTPEELERVHQRFLAVADAKKEVFDEDLHALMADERGTSNQTYDL
                     ESFRVTSGTGVCPTATVALRSGEAVLEQSASGDGPMDACFSAIDRITGLGVTLK"
BASE COUNT          645 a         1203 c         1287 g          677 t
ORIGIN      
        1 cttcggtcgg gttgcgcgct cctcctggcc ccctccgcca cgcgcgcgcc ccgaacgcgg
       61 agaccagcgc tgcaagaggc gtgccattgt caccgggctc aggcgtccgc ggcggcggtc
      121 tcggtgccgg cggtgcaaag gctgtgatag agggcttccg cggcgcgctg gttcatgccg
      181 gggcaggcgg cgagctcctc gagagtggcc tggcggatgc gggcgagcga gccgaagtgg
      241 cgaagcaact cgcgcttgcg cttcgggccg atgccaggaa cgctgtcgag cagggagcgc
      301 gtggatcgcc tggagcggcg cttgcgatgg tagctgatgg cgaagcggtg ggcttcgtct
      361 cgcacgcgct gcaagaggtg gagcgccggc gagtggcgtg gcaggcgcaa cgcctccggc
      421 tgcccaggaa ggaagatctc ctcgaactgt ttcgcgagcc caacctcggg gatctcgacc
      481 tccatctcgc gcatcgcctc cagcgcggcg ttcagctgcc ctttcccgcc atcgatgagg
      541 agcaggtcgg gcatctcgcg gaacttctca tcgcccatct tgccgcgcgc caggcgacgc
      601 ccgacgacct ccttcatcgc ggcgaagtcg tccggattag gccccaggct cttgatgatg
      661 aggcgccggt aatccgactt tctgggcgcg ccggcataca ggaccaccat ggcgccgacc
      721 gtctcaacgc cctgaatgtg tgagatgtcg taggcctcga tccgtagggg cactcgttcc
      781 atctcgagcg cctccttcag cgcggtgagc gcctcctgat tgcgcgcctt gtctgagtcg
      841 agcttcacct tgagctggcg gagcgcgtcc agggcgttgg tgtgcgccat ctccacgagc
      901 cgcttcttct cgccgcggga gggcgccgtg atatgcacct tcttgccgcg tttctggcgc
      961 aggaacgcct cgatgatatc gtgctcggcg atatcgcgct cgaccaggac ctccttgggc
     1021 gcataggttg cgtcctggta gtactgcttc aagaactccg ccgtcgcctg gctgagctcc
     1081 tcatccgtgg tgccctccag gtagaagtgc tcctggccga cgagccttcc attgcgcatg
     1141 aagaggagct gggcgcacgc ctggccatca tcgctcacca gggcgaccac gtcctgatcg
     1201 acgagggcgg tagagagaac cttctgtcgc tcccgaatcg tctccagcgc cttgatctga
     1261 tcgcgcaggc gggcggcccg ctcgaagttg aggcgttccg cggagcgctc catctcctgg
     1321 cgcagttgct tcagcagggc gtcttgccgg ccttccagga agaggcacgc ctcttccacc
     1381 gtctcgcgat actcctcctg gctgatcttc cccgcgcagg gaccgctgca ctcgttgatg
     1441 tggtagtaga ggcagagctt ctcgggctca ttccctgtca cctgatgatg gcacgagcgg
     1501 aggcggaaga tgcggctgag gagcgcgatg ctctgccgga cggcccgagc gtcggtgtag
     1561 ggcccaaagt agcggttgcc atcctttttg gcctcgcgga ctagcatcag ccgggggtac
     1621 ttctcggtca gcgtgacgca gatgtaggga tagtgcttgt cgtcgcgcag gcggacgttg
     1681 aaccgcgggc gatacttctt gatcaggttg cactcgagga tgagcgcctc gagctcagta
     1741 tcggtgatga tccaatcgat gtcggcgatc tcactcacga gccgccggac cttgggcgtg
     1801 tgatccgcgg aggcatggaa ataggagcgc acccggttgc gcagcgacac cgccttgcct
     1861 acgtacaata tttcgccggc cgcattcttc atcagatacg aaccgggccg gtccggtaga
     1921 atcgccagtt tctcctgcag cgtcatctcg tcccactcac cgtgccacgc ttcctcatta
     1981 ctttccatca taacaaagcg attcaccgtt gtcaaacgcg cgttcgcggg cgccaagatg
     2041 cttgacaggc gggcgtccga tgcatataat gaatactgac aaacgaatac gctcgaaaga
     2101 ctgtgaaagg gagtagtaag gcggcagtcc ggactgccag agagccgggt cgctgggaac
     2161 cggcgtcgca ggagccgccc gaactcgccc tggagttgcc cgccgaaacc ggtgccaggc
     2221 gcgccgggag tagtccggga cggtctctcc ccccgtcagc agggaggcgc tgtcgccccg
     2281 aggggccgca gcggcaaagg gggccgtgcg cgcgagcgcc ggtcaagcag agtggtaccg
     2341 cggaaggaat ggcctttcgt ctctgttgga gagacgaagg gctttttgca tttatggggg
     2401 gcgcgggagt caccgtgaag tccttcgggc gagcggcgat tggagggggc gcgagccatc
     2461 ctcgggagtg agaaatggaa cataaggtac acatctttga cacgaccctt cgcgacggcg
     2521 agcaatctcc cggcgccagc ctcaccgggc gtgaaaagct ggagatcgcg caccagctgg
     2581 cgcggctgaa cgtcgatgtg atcgaggcgg ggttcccctg ctcttccccg ggcgacttta
     2641 aggcggtgca agagatcgcc cgctcggtga agggacccgt gattacggcg ctcgctcggg
     2701 cggtgaaggc cgatatcgat gccgtgtggg agtcggtgcg cgaagcggag cgcccgcgca
     2761 tccacatcgt cctgggcagc tcggacatcc acgccaagca caagttccgg cgcagccgcg
     2821 aggagctcct cgccatgggc gttgaggccg tccgctacgc gcgcacgctc tgcccggacg
     2881 tcgagtactc caccgaggat gcctcgcgca gcgaccggcg ctacctctat caggtgctcg
     2941 aagcggtgat cgaggccggg gccacggtgg tgaatatccc ggacacggtg ggctatgccg
     3001 tgccggcgca gtggggtcgg ctcatcgcgg acatccggcg caacgtgcgc aacatcgacc
     3061 aggcgatcct cagcgttcat tgccataacg acctgggcat ggccgttgcc aactcgctgg
     3121 cggcggtgag ggcaggtgcc cagcaggtgg aatgcaccat caacggcatc ggtgagcgcg
     3181 ccggcaatgc ctctctcgaa gaggtggtca tggcgctgaa ggtgcgccgc aactactatg
     3241 gcgcgtacac cgacgtggtc accagcgagc tgtgccgcac cagcagcctg gtcagccggc
     3301 tgacgggcct caccgtggcg cgcaacaagg cggtggtggg cgcgaatgcc ttcgcgcact
     3361 cttcggggat ccaccaggat ggcgtgctca agaatcgcga caactacgag atcttcagcc
     3421 ccgacgatgt gggtgcctcc ggcacggaga tcatcctcac cgcccgatcg ggccggcacg
     3481 cggtgcggca tcgcctggag ggcctgggct acacgctcac tcccgaggag ctggagcgcg
     3541 tccatcagcg gttcctggcc gtagccgacg ccaagaagga ggtctttgac gaggacctgc
     3601 atgcgttgat ggcagacgag cgcggcacca gcaaccagac gtacgatctg gagagcttcc
     3661 gggtgacctc cggaacgggc gtgtgcccca cagcgaccgt cgcgctgcgt agcggggagg
     3721 cggttctcga acagtcggcc agcggcgatg ggcccatgga tgcctgcttc agtgccatcg
     3781 accgcatcac cggcctgggg gtgacgctga ag
//
LOCUS       DAOSCV010000391         3810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15193_length_3810_cov_3.188549, whole genome shotgun sequence.
ACCESSION   DAOSCV010000391 DAOSCV010000000
VERSION     DAOSCV010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15193_length_3810_cov_3.188549"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1183
                     /locus_tag="PLY56_10295"
     CDS             272..1183
                     /locus_tag="PLY56_10295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ21915.1"
                     /translation="MAKLLIAEDDHDAGALMKRVLMKGGHDVRLVDSGEAVLNACAED
                     APDVVLLDVSMPGMDGYEVARVLKSRPVTADIPIVFVTGSCETRQKVQGFSLGANDYI
                     TKPFHRDELVARVEVAIRIKQQSDALKAANAQLAALSVTDELTGLYNRRHLDQRLAEE
                     VALAKRHQFPLSCLMVDVDRFKRINDTFGHQEGDRVLQVVAQVLRESTRVSDVVGRYG
                     GEEFMVITPGTPLLSACKLGERIRTRTEAQDVQAAGDVIHITVSIGVAQLGQADTPES
                     LVASADRALYMAKRSGRNRVATEPQEA"
     gene            1229..1474
                     /locus_tag="PLY56_10300"
     CDS             1229..1474
                     /locus_tag="PLY56_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007141404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOJ21916.1"
                     /translation="MQEHYKRRATASRAYSDYSVVPLEDGTYRVEKVDGTTYLVNLDA
                     GTCTCPDFQRRIARREELAGVWCKHMHLCAASFGRSR"
     gene            complement(1484..2245)
                     /locus_tag="PLY56_10305"
     CDS             complement(1484..2245)
                     /locus_tag="PLY56_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21917.1"
                     /translation="MVNIIACNLGSYGRYRAVAYEHLRAIGLTNVEIPVPAPDALQGV
                     LDDLDRYGLKVTSVLGKCDLSNPACVEGLKPQAEAATRLGAKIVFLSVKTNGIPLSEA
                     YERLRGVGEVMAAAGLTAALETHPDLIQNASVALETMRAVDHPCVRVNFDTANIYYYN
                     EGCTAVEELRKIAPYVVSVHLKDTNGGYRAHHFPTLGQGVVDYPEVFRILNGQGMYGP
                     FTLELEGIAGEQLSEEQQKARVADSFAYLKQIGAA"
     gene            complement(2326..2607)
                     /locus_tag="PLY56_10310"
     CDS             complement(2326..2607)
                     /locus_tag="PLY56_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOJ21918.1"
                     /translation="MTCLSEDEATTRLQGLPGWRICENGIRKTYAFRSFREAIEFTQA
                     VAEIAEAYKHIPLIEIRRNEVTITFPPQGGCGISDLDIALATHVDLAQE"
     gene            complement(2777..3655)
                     /locus_tag="PLY56_10315"
     CDS             complement(2777..3655)
                     /locus_tag="PLY56_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ21919.1"
                     /translation="MGLQLSGVRISAAGKAVPPKGWVCPRRTLSEHLLLYVRKGTGTL
                     QAGEQTLALRGSHLCLIRAGTPHLLGADSGRVLEHWYLHFVLLDAHGAALAPEALRLP
                     EAVVLPARRATEREFLRCVLCAGEQGCVSAAGMLASFFTVLEQVLQEGTGAAGGEGAA
                     SLQGRGTQARRSAQVMEAVELMMRHVSDDLSMEEIAAQVGMSASHFEKVFRRFTGTTP
                     YTYYLGVKMERAKALMAASNRRLSDIAIELGFSSLHHFSRAFKQYEGELPSRYAARVR
                     PPRPKGSGRAPQDANL"
BASE COUNT          684 a         1251 c         1197 g          678 t
ORIGIN      
        1 cttcgctcgc aagcagcgcg gtgatttatc gccgcgcgcc ggattgcgcc ctgcctccgt
       61 ggcttcggtt tccttatgtc ggaatcccag gggttcccga tgcggggttg ctggcaacgg
      121 aaggagattc tccggccgaa agcaaacctt cctacagggc atcgcttgtt ccgggggagg
      181 gctagctttt tcctcctgac gcgggccatt gaaagccctc gaggagctcc tgcctgcccc
      241 tgatgggata gtgcctcaca ggtggagatc catggctaag ttgctcattg cagaagatga
      301 ccatgacgcg ggcgctttga tgaagcgtgt cctgatgaag ggggggcacg acgtccggct
      361 ggtggatagc ggggaagcgg tgctgaacgc ttgcgccgag gatgcccccg acgtggtgct
      421 gctggacgtc tccatgcccg ggatggacgg ctacgaggtc gcccgcgtcc tgaagagtcg
      481 gccggttacc gccgacatcc ccatcgtctt cgtgaccgga tcttgcgaaa cgcgccagaa
      541 agtgcagggc ttcagcctgg gggcgaatga ttatatcacc aagcccttcc accgcgatga
      601 actcgttgcc cgcgtcgagg tggctatccg catcaagcag caatcggatg ctctgaaggc
      661 agccaacgcc caactcgccg cgctttccgt caccgacgag ctgactgggc tctacaaccg
      721 ccgccatctg gatcaacgcc ttgccgagga ggtggcgctc gctaagcggc accagttccc
      781 gctctcgtgc ctgatggtgg acgtggaccg gttcaagcgc atcaacgata ccttcggcca
      841 ccaggagggc gaccgcgtgc tccaggtcgt cgcgcaagtt ctccgggaat ccactcgtgt
      901 atccgatgtt gtgggccgct acggcggtga agagttcatg gtgatcacgc ccggcacgcc
      961 gctgctctct gcctgcaagc taggcgagcg cattcggact cggacggagg ctcaggatgt
     1021 ccaggccgcc ggtgatgtga tccatatcac ggtaagcatc ggggtagccc aactcggcca
     1081 ggcagatacg ccagagagtc tcgtcgcgtc cgccgaccgg gcgctctata tggccaagag
     1141 aagcgggcgc aaccgcgtcg ccacggaacc gcaggaggcg tagcgcgaga tagcgccttg
     1201 gatcaggggg aaggcggtga acaggcgcat tcaggagcat tacaagcgac gggcgaccgc
     1261 cagccgcgcg tatagcgatt actcggtggt gccactggaa gatggcacct atcgggtcga
     1321 gaaggtagac ggcaccacct acctcgtgaa tctcgatgcc ggcacctgca cctgtcctga
     1381 cttccagcgg cgcatcgccc gccgcgagga gctggccggc gtctggtgca agcacatgca
     1441 cctctgcgcg gccagcttcg ggcgctctcg ctagtggcgc ctatcaggcg gcgccgattt
     1501 gcttcaggta ggcgaaggag tctgccaccc gcgctttctg ctgctcctcc gaaagctgct
     1561 ccccggcgat cccctccagc tccaaggtaa acgggccgta catcccctgc ccgttcaaga
     1621 tgcggaagac ctctggataa tccacgactc cctgacccag cgtggggaag tggtgggcgc
     1681 ggtagccccc gttcgtatcc ttcaggtgca cactcaccac gtagggcgcg atcttgcgca
     1741 gctcctccac ggccgtgcag ccctcgttgt agtagtagat gttcgccgta tcgaagttga
     1801 cgcgcacgca cggatggtcc accgcgcgca tcgtctccag ggcgacgctg gcgttctgga
     1861 tcaggtctgg atgcgtctcc agcgcggccg tcagccccgc ggcggccatc acctcgccca
     1921 cgccgcgaag gcgctcatag gcctccgaca gcgggatgcc gttcgtcttc acgctgagga
     1981 atacaatctt agcgcccagg cgcgtggcgg cctctgcctg cggcttcagc ccctcaacgc
     2041 acgccggatt cgacagatcg cacttcccca ggaccgaggt caccttcaag ccgtaacggt
     2101 ccagatcatc cagcaccccc tggagcgcat ccggcgccgg cacggggatc tcgacgttag
     2161 tcaggccgat ggcgcgcagg tgctcatatg ccactgcccg atagcgacca tagctgccca
     2221 ggttacaggc gatgatgttg accatagctg ccccttcctt gcgcctgccc ggcgaactac
     2281 cgggcaggat agaccgcgtc gatagggact tctctggcca aggcgtcact cctgcgccag
     2341 atccacgtga gtcgctaggg caatatcgag gtcggaaatg ccacaaccgc cttgcggggg
     2401 aaaggtgatc gtcacttcgt tgcggcggat ttcgatcaga gggatgtgct tgtacgcctc
     2461 cgcaatctcg gcgacggcct gcgtaaactc aatcgcctcc cggaaactgc gaaacgcgta
     2521 cgtcttccga atcccgtttt cgcaaatcct ccaccccgga agtccctgga ggcgcgtggt
     2581 ggcctcatcc tccgagagac atgtcatcgt catcctcctc ccggcgcgct gctgctcggc
     2641 gtccgctctc agccaggcaa gaactgcgcc atatgccggc gcttcgcggg aggaggggcc
     2701 cctcagagcc agcgtctctc actctccacc gcggggagga tccgtcgagg gcgttagcta
     2761 cacgacgctc cgacggtcaa aggttggcat cctgtggagc gcggcccgat cccttggggc
     2821 gtggagggcg aacgcgggcg gcatagcggg agggcagctc gccctcgtac tgcttgaacg
     2881 cgcgcgagaa atgatgcaag ctggagaaac cgagttcgat ggcgatatcg ctgagacggc
     2941 ggttggaggc ggccatcagc gccttggccc gctccatctt cacgccgagg taataggtat
     3001 agggagtggt gccggtaaag cgtctaaaca ccttctcgaa atgcgacgcg ctcatgccca
     3061 cctgcgcggc gatctcctcc atcgagaggt catcggagac gtgacgcatc atcagctcga
     3121 cggcttccat cacctgtgcc gagcggcgag cttgcgttcc tctgccttgg agcgaggccg
     3181 cgccctcccc cccggccgcc ccggtcccct cctgcaacac ttgctccagc acggtaaaga
     3241 aggatgcgag cattcccgcc gcggagacgc atccctgctc ccccgcgcac aggacgcagc
     3301 gcagaaactc tcgttccgtg gccctgcgcg cgggcaacac caccgcctcc ggcaggcgca
     3361 gcgcctctgg ggcgagcgcc gcgccatggg catccagaag cacaaagtga aggtaccaat
     3421 gctccagcac ccggccggaa tcggcgccga ggaggtgggg agtgccagca cggatcaggc
     3481 agaggtggct gccgcgcaac gcgagagtct gctcgccagc ctgaagcgtt ccggtgcctt
     3541 ttcgcacata gagaagcaga tgctccgaga gcgtccgccg ggggcagacc cagcccttgg
     3601 gaggcaccgc tttgcctgcc gccgagatcc gtacaccact aagctgcaac cccattgccc
     3661 gcgtttcccc tggtcatcct cagccctact ccgctcacgg ggtggctcct cccccgcaga
     3721 cggaacgatc tctcgtagta taggcgcttg ccagcgggca tgtcaaggag ggtgtaggtt
     3781 cggcaccggg cgggggaagt ggttcggcag
//
LOCUS       DAOSCV010000392         3799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15249_length_3799_cov_4.333868, whole genome shotgun sequence.
ACCESSION   DAOSCV010000392 DAOSCV010000000
VERSION     DAOSCV010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15249_length_3799_cov_4.333868"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="PLY56_10320"
     CDS             <1..1130
                     /locus_tag="PLY56_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ21920.1"
                     /translation="RRLPFTTKEDMRAAYPYELFAVPLREIVRIHSSSGTTGRATVVG
                     YTRNDLRTWSELVARIISAVGVTDQDVVQITFGYGLFTGGFGLHYGAERVGASVIPVS
                     SGNTERQIQIMRDFRTTALVGTPSYALHIAEVMKERGIDRSSLCLRVGLFGGEPSSEQ
                     MRKKLEESLDIMATDNYGLSEVLGPGVAGECECQCGMHINEDHFIAEVIDPQTLEPVP
                     YGQEGELVLTTLTKEGIPVIRYRTRDLTSLNPEPCRCGRTNVRMKKVLSRTDDMLIVR
                     GVNVYPSQIEQVLFEVEGVEPHYQIVLERRAALDEMTILVEVSRELFTGQMSRLVELE
                     TKIRSRMHSVIGITPRLKLVEPRSLERSLGKARRVVDNRKI"
     gene            1666..3147
                     /locus_tag="PLY56_10325"
     CDS             1666..3147
                     /locus_tag="PLY56_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096537.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOJ21921.1"
                     /translation="MLGIGYYCMRRTRTVSDFFLAGRNLGPWMSAFAYGTSYFSAVLF
                     VGYAGRLGWGFGLNALWIAAGNVLFGSILAWWIFAGPTRRMTARLDALTMPEFLGARF
                     GSPFLKFASALVIFVFLVPYSASVYQGLSLLFEKNLGIPYGLAVGFLAFLTGVYLVMG
                     GYLALAVTDFLRGIIELAGVVVMVLFLVHLGGGMSAAVSTLSKPEMAPALSAPGPFPG
                     WVVLWSLVFVTSFGPWGLPQMVQKFYSIKSEADVKRAMVVASAFALVLSFGAYFTGAL
                     THLFYYAPVEGVPAPAGMTPVPANLDDLMPHFLTHYVPAWVSMVILLLVFSASMSSLS
                     SLVLVSSSAIAVDLYAGLRKDASKESVMLLMRVLCGVFVAISLLMALKKVDFIVNLMV
                     ISWGALAGAFLAPYCFGLFWKRTSKAGAIAGMLSGLATMVLIYAKYGGVGVPVAGAAG
                     MVVPVIVVPLVSLLTPPPDREILDRAFGEAVEPTAPEPEMVEV"
BASE COUNT          572 a         1097 c         1372 g          758 t
ORIGIN      
        1 ttcgccggtt gccgttcacc accaaggagg atatgcgtgc ggcataccct tacgagctgt
       61 tcgccgtgcc gctgcgagag attgtgcgca ttcactcctc gtcgggcacg accgggcggg
      121 cgacggtcgt gggctacacg cgcaatgacc tgcgcacctg gtccgagctc gtggcgcgca
      181 tcatcagcgc cgtaggcgtg acggaccagg acgtggtgca gatcaccttc ggttacgggc
      241 tgttcacggg tgggttcggg ctgcactatg gagcggagcg cgtgggcgct tccgtgatcc
      301 cggtctcgtc gggcaacacc gagcgtcaga tccagatcat gcgggatttc cgcacgacgg
      361 cgctggtggg caccccgagt tacgcgctcc atatcgccga ggtgatgaag gagcgtggca
      421 ttgatcggtc gtcactctgc cttcgagtgg gccttttcgg cggcgagccc tcttccgagc
      481 agatgcgcaa gaagctggag gagagcctcg atatcatggc gacggataac tacgggctca
      541 gcgaggtgct gggtccgggc gttgccggcg agtgcgagtg ccagtgcggg atgcacatca
      601 acgaggacca cttcatcgcg gaggtgatcg acccgcaaac cctggagccg gtgccctacg
      661 gccaggaagg cgagctggtg ctgacgacgc tcaccaagga aggcatcccc gtgatccgct
      721 accggacgcg cgacctcacc tcgctcaacc cggagccgtg ccggtgtgga cgcacgaacg
      781 tgcgcatgaa gaaggtcctg agccggaccg acgacatgct gatcgtccgc ggcgtgaatg
      841 tgtatccctc gcagatcgag caggtcctct tcgaggtaga gggcgtggag ccgcactacc
      901 agatcgtctt ggagcgccgc gccgcgctgg atgagatgac gatcctggta gaggtctcgc
      961 gcgagctgtt cactggccag atgagccggc tggtggagct ggagacgaag atccgatccc
     1021 ggatgcactc ggtgatcggc atcacgccgc gcctgaaact ggtggagcct cggagcctgg
     1081 agcgtagcct cggcaaggcc cgccgcgtgg tggataaccg taagatctaa agccacagag
     1141 gggatgccca gcccgcgtcg gaagtggccg gcttggggcc ggccgtaagc aatgcgcggg
     1201 cgggcgtgcc cgcccttgtc ccgcaccgca atggatcggg gccgtgcccc tccccgttcg
     1261 gattctcgag tacctcctgc gggtgccggg gatgggggcg gctagcctgg cggatgtggc
     1321 catctcggtc gcgagcggcg caagaggcgg cgctgactgc gggcaggagt tctgtgccgg
     1381 gatggtgaat gctcacccca gggtccatca tcggtggata gcatggttct cctcgtctct
     1441 tccaaagcct cgattaacga cccccagatg tgaaggggat aacctgcgtt gcttgcttcc
     1501 cctcgtgcct ggtgaagagg aaagggcgac cctccggaat ggactctaag cgggcgtgtg
     1561 tggcactctg ggatgcgacg gcagcgcgag tgcatggtat ggcccacggg gaaaggtgag
     1621 gatctgttgt ttaagctgct gattgtcgtg gtttatgtgg cgctgatgct tggcatcggc
     1681 tactactgta tgcgcaggac gcgcaccgtg agcgatttct tcctggcggg gcgcaatctg
     1741 ggaccatgga tgtcggcctt cgcctatggc accagctatt tctccgcggt cctgttcgtg
     1801 ggctacgcgg ggagattggg gtggggcttc ggcctgaacg cgctctggat cgccgccgga
     1861 aacgttcttt tcgggagcat cctggcgtgg tggatcttcg cggggcccac gcgacgcatg
     1921 acggcacgcc tcgatgcgct caccatgccc gagttcctgg gcgcccggtt cgggtcgccc
     1981 ttcctgaagt ttgcctcagc gcttgttatt ttcgtcttcc tggtgcccta ctccgcgtca
     2041 gtctatcagg gactgtcact tctcttcgaa aaaaacctgg ggatcccata tgggctcgcc
     2101 gttggctttc tggcgttcct aaccggggtg tacctggtga tgggggggta tctggcgctt
     2161 gcggtcaccg acttcctccg agggattatc gagctggcgg gtgtcgtggt gatggtgctc
     2221 ttcctggtac acctcggcgg cgggatgagc gcggcggtga gcacgctttc gaaaccggag
     2281 atggcaccgg cgctgagcgc gccagggccc ttccccggct gggtggtgct gtggtcgctg
     2341 gtgttcgtga cgagcttcgg gccgtggggc cttccgcaga tggtgcagaa gttctactcg
     2401 atcaagagcg aggcggatgt gaagcgggcg atggtcgtgg catcggcgtt cgcgctcgtc
     2461 ctctcgtttg gtgcctactt caccggggcg ctcacgcacc tgttctacta tgcgccggtg
     2521 gagggcgtgc cggcgcccgc cgggatgact ccggtgccgg ccaatcttga tgacctgatg
     2581 ccgcacttcc tcacgcacta cgtgccggca tgggtgtcga tggtgatcct tctcctggtc
     2641 ttctccgcat cgatgtcgag cctctcctcg ctcgtcctgg tctcgagctc ggcgattgct
     2701 gtggacctgt atgcggggtt gcgcaaggac gcgtcgaagg agagcgtcat gctcctgatg
     2761 cgcgtgctat gcggagtgtt cgtggcgatc tcgctgctga tggcgctgaa gaaggtcgat
     2821 ttcatcgtca acctgatggt gatctcgtgg ggcgcgctcg cgggcgcgtt tctggcgccg
     2881 tattgcttcg gactgttctg gaagcgcacc tccaaggctg gggcgattgc cgggatgttg
     2941 tcgggcctgg cgacgatggt cctgatctac gcgaagtatg gtggggtggg cgtgcccgtg
     3001 gccggcgcgg cggggatggt ggtcccggtg attgtcgtgc cgttggtcag cctgctgacg
     3061 ccgcctccgg atcgggagat cctcgaccgg gcgtttgggg aggctgtcga gccgacggcg
     3121 cccgagccgg agatggtcga agtgtagccg cgcgcaggct tgcgactgcg ggcagggatg
     3181 cggggtcaga aagccccctc cctgcccttc gtgtttgtag ggcctcgcgc gtttcacgtg
     3241 aaacgttggc gagggccgcg tcaccgggtg atcgtcggtg gcattcggag tcataccggg
     3301 gggctgcttg aggggtgtct gttgccgacc ggcggtcgct ccccttccct ggggtcagcc
     3361 cgggtttgaa tgtccgggat agggccggag tctgggcgaa ttgccgggca ggcatcggca
     3421 cgacagatca gggggcgaag atagaggggg atgcgtgtgc atcggggatc gcgccgcccg
     3481 accgggcgtg tatgctcccc cggtggcttc ctgcctctgg ccggcgcgcg gccgagaggt
     3541 ggggagagga cctggggaca ggaaggcagg cctgggcccg actgggatcc gacataagga
     3601 atccgaagcc tcggaggcag ggcgcaaccc ggcgcgcggc gataaatcac cgcgctgctt
     3661 gcgagcgaag ccctgccggg ctgggttggg gatactccct gatgcctcaa gacggcggtg
     3721 gctgggaggg gaagcaccga aggagctttg ccggaaagca gcgcggagct tcgagctccg
     3781 cgcgtgcggg cggccttct
//
LOCUS       DAOSCV010000393         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15305_length_3790_cov_3.172423, whole genome shotgun sequence.
ACCESSION   DAOSCV010000393 DAOSCV010000000
VERSION     DAOSCV010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15305_length_3790_cov_3.172423"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLY56_10330"
     CDS             complement(<1..773)
                     /locus_tag="PLY56_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ21922.1"
                     /translation="MKLTFLGTSAGEQYPGFWCRCEYCDKARQLGGRNLRKNSCAWLD
                     PDTLIDFPPEIFMQAERFGIPVVDARTILITHSHQDHFYPNLFHWRAMRPDIPLPPPH
                     TIGGPRFSPVVPLRVYGNQQVCDAVLARVGDRLELCAMEVHQVEPFREIEEGAMRFVA
                     VPGNHADGDGHALTYIIQREGKTFLYALDTGWFLPESYAEIARHRYDLVVIEGTFGFG
                     VDSEQHMNFRKLEEVRRLFEIFFFFNDTATTEIYTSVHTL"
     gene            complement(1262..>3790)
                     /locus_tag="PLY56_10335"
     CDS             complement(1262..>3790)
                     /locus_tag="PLY56_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ21923.1"
                     /translation="DRFERADGTVDWVRWEIRPWYTISGKVGGVTMFTEVITERKHVE
                     DAIRASETRFRILSTSAPIGIFELDAEGRCQYVNTRWEAITGRTSEEGLGYGWHQTIH
                     PEDREWLLQEARERIRAGFPGEREFRILRPDGSVRWVRVHVAPMVDPQAGRPSGFVGT
                     LDDITAEKEAEEKLQASEQRFMALVNSNIVGIVLGDMSGRIYKANDAFLKMAGYTRAD
                     LAAGKVRWSLLTSPESAPEDQRALAELQEKGTAGPWEKEYTRKDGSRVPVLIGLARLG
                     ATGSQTIGFVLDLSERKRAEGALRLIAQASRVLASSPDFPDAVNRIAHLLVPTLGDWC
                     VIHVLDEEGNVQRAAVAAADPAQASLARQWMEAPPPAPEAIQPLLQALRTGKTQLLKG
                     VSDELWPAMINSEARVAVMRQVGATSMLVVPFIVRGHLTGWASFLTTRPGRTYQPPDI
                     TLAEEVAHHVAQALDNARLYQEAREANAAKDQFLAVLSHELRNPLSPILTGVEVLRRS
                     IPLDPRTERVLTLIERNAELQARLITDLLDFSRIRRGAIGIERSPVPLDAAVNAAVES
                     TQGEAKAAGLAVKLEIQPGVWVLGDLDRLQQIVVNLLTNAIKFTPPGGEVRVRVERGE
                     HVGRVIVEDTGVGIDAALMPRLFEMFQQGEIEGQRARGLGIGLALVKHLTELHGGRVW
                     AESEGVGKGSRFTVELPLAPAAAREPAEKEAREPMAVRILLVEDNADARELLQAGLEL
                     LGYTVEAVGSGEEALALLSEMRPDVIVADIGLPGVDGYEFLRQARRIPGLANVPAFAA
                     TGYGRPEDVARAHEAGFTDHFVKPVDIADLDARIRSYLEMKQRE"
BASE COUNT          659 a         1315 c         1155 g          661 t
ORIGIN      
        1 agagtgtgaa cgcttgtgta gatctcggtg gtcgccgtat cattaaaaaa aaaaaaaatc
       61 tcgaagaggc gccggacctc ttccagcttt cggaagttca tgtgttgctc ggagtccacc
      121 ccaaacccaa aggtcccctc gatgacaacc aggtcgtatc ggtgccgggc gatctcggcg
      181 tagctctccg gaaggaacca gcccgtgtcg agggcataaa gaaacgtctt cccctcgcgc
      241 tggatgatgt aggtgagcgc gtgtccgtcg ccgtccgcgt gattcccggg aacggccacg
      301 aagcgcatcg cgccttcctc tatctcgcgg aagggttcca cctggtgcac ctccatcgcg
      361 cacagctcca ggcggtcacc cacccgggcg agcaccgcgt cgcacacctg ctgattgccg
      421 tacacgcgca ggggcacgac tggcgagaag cgcggtcccc cgatcgtgtg tggcggggga
      481 agcggtatgt cggggcgcat cgcgcgccag tgaaagaggt tcgggtagaa gtgatcctga
      541 tgcgaatgcg tgatcagaat cgtgcgcgca tccaccaccg gaatgccgaa gcgctccgcc
      601 tgcatgaaga tctcgggcgg gaagtcgatc agggtatcgg gatcgagcca ggcgcacgag
      661 ttcttgcgga ggttgcgacc ccccaattgg cgcgccttgt cacaatactc gcagcgacac
      721 cagaagccgg ggtactgttc tccagccgag gtgccaagaa aggtgagttt catctctcgc
      781 gcttctttct atgaagcaag cagatccctc gtgccacgtg gttcgcgtat ccgccggcgc
      841 gctacatcac gaagcacgag gagcagaccg acactcctct tccaaccctg cccactctcc
      901 cggggggaga gcacagtcgc ccgcgcgact acaggcagac gcggccgggt ggccgggtga
      961 ggaatcgccg ggcaaggtgg caggccggcc catcgcgtca ggttgcaggg cccccggtgg
     1021 cagcccagtt cgctatgggc gtgcccgccg tgttggccct ctccgcatcg cctctcgttt
     1081 cggcggcagc gcctgcgctc ctgccgcgaa ccgcccacgc gcccatccgc gaggcgggat
     1141 ggacctgaat gcgccagcga aatggaatcg gtgagcggat gggcggaaac ggacgcaccg
     1201 tggggggggc gctccccgtt cggcacgccc cccagccact tcctggagcg gccgggcatg
     1261 cctattcgcg ctgcttcatc tccagatagc tgcggatgcg cgcgtccagg tcggcgatat
     1321 cgaccggttt gacgaagtga tccgtgaagc cagcttcatg cgcccgcgcc acgtcctcgg
     1381 gccgcccata gccggtggcg gcaaaggcag gcacattcgc caggcccggg atgcggcgag
     1441 cctgccgtag aaactcgtag ccatccacgc cgggcaggcc gatatccgcc acaatgacat
     1501 ccgggcgcat ctccgagagc aacgcgagcg cctcttcgcc gctacccacc gcctcaaccg
     1561 tgtagcccag caactccagg cccgcctgca ggagctcacg ggcatcggcg ttgtcttcca
     1621 ccaggagaat cctgaccgcc atcggctccc gcgcctcttt ctcggctggt tccctggccg
     1681 cggcaggagc caggggcagc tcaaccgtga aacggctccc tttccctacc ccttcgctct
     1741 cggcccacac tcgcccgcca tgcagctctg tgaggtgctt gacgagggcc aggccgatcc
     1801 ccaggccacg ggcgcgctgc ccctcgatct ccccctgctg gaacatctca aagagccggg
     1861 gcatcagcgc tgcgtcgatg cccactcccg tgtcctcgac gatcacccgg cccacatgct
     1921 ccccgcgctc gacgcgcacg cgcacctcgc ccccgggcgg tgtgaatttg atcgcattgg
     1981 tgaggaggtt caccacgatc tgctgcaggc gatccaggtc acccaggacc catacccccg
     2041 gttggatctc tagcttcacc gccaaccccg ccgccttcgc ctcgccctgc gtcgattcga
     2101 ccgcggcgtt caccgcggca tcgagaggaa caggtgagcg ctcgatgccg atggctccgc
     2161 gccggattcg cgagaaatcg agcagatcgg taatcaggcg ggcttgcagc tcggcattcc
     2221 gctcgatcag ggtcagaacg cgctcggtgc gcggatccag ggggatgctg cgccggagta
     2281 cctccacgcc cgtcaggata ggcgagagtg ggttgcgcaa ttcatgcgag aggacggcga
     2341 gaaactggtc tttggcggca ttggcctcgc gcgcctcctg gtaaagacgc gcgttatcca
     2401 gtgcttgcgc cacatggtgg gccacttcct cggccagggt gatatccgga ggctgatacg
     2461 ttcgtcccgg ccgggttgtc agaaaactcg cccatcccgt gaggtgtccg cgcacgatga
     2521 agggcacgac caacatcgag gtcgccccca cctggcgcat gacggccacg cgcgcctcgc
     2581 tattgatcat cgctggccac aactcgtccg aaacgccctt cagtagctgg gtcttgccgg
     2641 tgcgcaaggc ctgcaagagt ggctggatcg cctccggcgc gggcggaggc gcctccatcc
     2701 attgacgagc aaggctcgcc tgcgccgggt ctgccgcggc aacggccgcg cgctgcacgt
     2761 tgccctcctc atcgagcacg tggatgacgc accaatcacc cagcgtgggc accaggaggt
     2821 gagcgattcg gttgacggca tccggaaaat caggcgacga agccaacacg cggctcgcct
     2881 gggcgatgag gcgcagcgcc ccttccgcgc gctttcgctc gctcaggtcg agcacgaaac
     2941 cgatcgtctg cgagccagtc gcccccagcc ttgccagtcc gatgagcacc ggcacgcggc
     3001 tcccatcctt gcgagtgtac tccttttccc agggtccagc cgtgcccttc tcctgcaact
     3061 cggcgagtgc ccgctggtcc tcgggggcgc tctccggcga ggtcaagagg ctccaacgca
     3121 ccttgcccgc agccagatct gcgcgggtat aaccggccat cttcaggaag gcatcgttcg
     3181 ccttatagat ccgcccgctc atgtcgccga gcacgatccc gacgatgttg gagttcacca
     3241 gcgccatgaa acgctgctcg ctcgcctgca gcttctcttc cgcttccttt tccgcggtaa
     3301 tatcatctag cgtgccaaca aagccagagg gtcgcccagc ctgggggtcg accatcggcg
     3361 ccacatgcac gcgcacccag cgcacgctcc catcggggcg caggatccta aactcgcgct
     3421 cgccgggaaa gccggcgcgt atcctctccc tggcctcctg aagcaaccac tcccgatctt
     3481 ccgggtgaat tgtctgatgc cagccatagc cgaggccttc ctccgacgtt ctcccggtga
     3541 tcgcctccca gcgcgtgttt acgtactggc accgaccttc ggcgtccagc tcaaagatcc
     3601 cgatgggggc ggaggtactc aggatacgga agcgggtctc gctggcgcgg atcgcatctt
     3661 ccacgtgctt gcgctcggtg atcacctcgg tgaacatcgt gaccccgccc actttcccgc
     3721 tgatcgtgta ccagggccgg atctcccagc gcacccagtc tacggtgcca tcggcgcgtt
     3781 caaagcgatc
//
LOCUS       DAOSCV010000394         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15323_length_3787_cov_3.606913, whole genome shotgun sequence.
ACCESSION   DAOSCV010000394 DAOSCV010000000
VERSION     DAOSCV010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15323_length_3787_cov_3.606913"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2295
                     /locus_tag="PLY56_10340"
     CDS             <1..2295
                     /locus_tag="PLY56_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21924.1"
                     /translation="VTLARGDESYVRSEKLKRYADLATVIQNNHFELVPFGDCGGYHT
                     RGTGATSILLRAAEWHHHPGYKWLAERHAPEAAAADILAADVPAAPPAEHVGLFVLPL
                     DPVIHRWTTLPRFPGYPLPNVLPNVPAAEGFDKLSLRGGWGPDDDYLLLQGFGDGQHG
                     HPDANAISQYQVKGRLFLAESDYIRRMPKQHNMVMVIRDGAHAPIPVTARLDGAREFP
                     WGAITQTSLLDYNGCDWQRTLIWLRGDCVLCVDALRARVAGDYELRCYWRTLGDAELT
                     EQGLHAEHTGEHFHILARGEPERRLDIEPVPLNTTRYPEYRYGDPRPKVLQERLRVHL
                     DAGEEACFVNLLLPNGEHAAPRRTLERPGAGQIRIGGEGPAVEVDLDGVQVAGHTRVT
                     FPSGARLTALAAAPAAITGAACLPDTLAGPGRRIALPAPVTALTPLEDGAVLAGCADG
                     SLLRVEASGKTVLLAKAGDRVGVVAAGRLWGEEELTLLAASYDATLRLLRPDGEPRLT
                     VELPANGHMPAWGKAICLANLDGDGRLWPVAGTAAWRVHALLPDGGFRWTADTAAHAT
                     TCLARGDLNHDGRDEIVAGTVYFCVPAFTADGERLWQDEDYNDYWQAGPTFPFVAVAD
                     VDADGDLEVLTVGSDTLVHCISHLGEKKWTHSIGDEAAGLVVLPQGIVAASLTGDLHL
                     IDGRGNRAWRRELGSPCTALTQAGHGVCVATEAGDLVWVDAASTPLSRWRLPAPAECL
                     LGTHAAIWAGTRDGALCYLDLPPR"
     gene            complement(2481..3602)
                     /locus_tag="PLY56_10345"
     CDS             complement(2481..3602)
                     /locus_tag="PLY56_10345"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ21925.1"
                     /translation="MERLLTMIRKEFQQLRRDRRTLALFTVAPVLQLIIVGYAVSNDV
                     RNVPLAVYDGDRSQQSRALVQAVAASQHFIIHPVENPRDLPRMLAHRRAEVALAIPPE
                     FTRRILRGETPSVQVLVDGSDSTSATLAAEYLALDLQAAHSSLMIETLRRPRDEGTRP
                     GVSPQPRVWFNPQLLSTHYMVPGVLALVLVVTTTIATSLSIVREREVGTLEQLVVTPL
                     RGWELLAGKMIPLGILALLDAAIILAVIRFWFRVPMLGSPGLVLICVLGLMLNTLGTG
                     LLISTISRTQQEAQLTAFLLVNPSILLSGFIFPVENVPALLRPLSYAIATTHFLKAER
                     AIFLRGAELADVLPQLLWMLGLGMLIFALGMWRFRKQVD"
BASE COUNT          621 a         1244 c         1349 g          573 t
ORIGIN      
        1 gtgacgctcg cccggggcga cgagtcgtac gttcgcagcg agaagctgaa gcgctacgcg
       61 gatctggcca ccgtgatcca gaacaaccac tttgagctgg tgccgttcgg cgattgcggc
      121 ggctaccaca ccagaggaac cggggccacg agcatcctcc ttcgcgcggc ggagtggcat
      181 caccatccgg gttacaagtg gctcgccgag cgccacgcgc cggaggccgc cgcagccgac
      241 atcctcgccg ccgacgtgcc cgcagcgccg cctgcggagc acgtgggcct cttcgtgctc
      301 ccgctcgacc cggtgatcca ccgctggacg acgctcccgc gctttcccgg ctatccgctc
      361 ccgaatgtgc tgccgaacgt gccagccgcg gaggggtttg ataagctctc gctgcgcggg
      421 gggtgggggc cggatgacga ctacctcctc ttgcagggct ttggcgatgg gcagcacggc
      481 cacccggatg ccaacgccat cagccagtac caggtgaagg gccggctctt cctcgccgag
      541 agcgactaca tccggcgcat gccgaagcag cacaacatgg tgatggtgat ccgcgacggc
      601 gcgcacgccc cgatcccggt aactgcccgg ctggacggcg ctagggagtt tccgtggggt
      661 gccatcacgc agaccagcct gctcgactac aacggctgcg actggcagcg cacgctgatc
      721 tggcttcgcg gcgattgcgt tctgtgcgtg gatgcgttgc gcgcgcgcgt tgccggcgac
      781 tacgagcttc gctgctattg gcgcaccctg ggcgacgcgg agctgacgga gcagggcctc
      841 cacgccgagc acacgggcga gcacttccac attctggcac gtggcgagcc ggagaggcgc
      901 cttgatatcg agccggtgcc gctgaacacg acgcgctatc ccgagtatcg ctatggcgac
      961 ccgcgtccca aggtgctcca ggagcgcctg cgggtccatc tcgatgccgg tgaagaggcg
     1021 tgcttcgtca acctgctcct cccaaatggc gagcatgctg cgcccaggcg caccttggag
     1081 cgccccggag ccgggcaaat ccggattggg ggagaaggtc cggcggtcga ggtggacctg
     1141 gacggagtgc aggtggcggg ccacacgcgt gtcaccttcc cgtccggagc gcgactgacc
     1201 gcgctggcgg cggcccccgc cgcgatcacg ggcgcggctt gcctcccgga cacgctggcc
     1261 gggccaggcc ggcgcatcgc gcttcctgcc ccggtgacgg cgctcacgcc gctcgaggat
     1321 ggcgcggtcc tggccggctg cgcggatggc agtctcctgc gggtggaggc gtcgggaaag
     1381 acggtgctcc tggcgaaggc gggcgaccgc gtcggcgtgg tggccgccgg acggctatgg
     1441 ggcgaggagg aactcaccct tctggccgca tcctatgatg ccaccttgcg cctcttgcgg
     1501 ccggatggcg agccgcggct gaccgtcgag ctccctgcca atgggcacat gccggcctgg
     1561 gggaaggcga tctgcctggc caacctggat ggcgatggcc ggctttggcc cgtggccggc
     1621 accgccgcgt ggcgagttca cgcgctcttg ccggatggcg gcttccgctg gaccgccgac
     1681 acggccgccc acgcgaccac ctgcctggcc cgcggagacc tcaaccacga tggccgcgat
     1741 gagatagttg ccggcacggt ctacttctgc gtgcccgcct tcacggcgga tggcgagcgg
     1801 ctatggcagg atgaggatta caacgactac tggcaggcgg gtccgacctt cccctttgtg
     1861 gccgtcgccg acgtggacgc cgatggcgac ctggaggtcc tcacggtggg cagcgacacc
     1921 ctggtgcact gcatcagcca cctgggggag aagaagtgga cgcacagcat cggcgacgag
     1981 gccgccgggc tcgtggtctt gccacagggg atcgtggcgg cctcgctcac cggggatctc
     2041 cacctgatcg atggccgagg caaccgcgcg tggcgaagag agctgggcag cccctgcacc
     2101 gccctgaccc aggctggcca cggggtgtgc gtggccacgg aggcaggtga cctggtgtgg
     2161 gtggatgccg cgagcacgcc cctctcccgg tggaggctgc ccgccccggc ggagtgcctc
     2221 ctgggaaccc atgcggcgat ttgggccggc acgcgcgacg gggctctctg ctacctggac
     2281 ctcccgccgc gctagcccga agtattcacc agaatacttc aagagaagac tcttgcctgg
     2341 tagcacgccg ttgccgccac aaacgcccgg cggcagagtg ttttcggggc agtgcccgcg
     2401 aacccgtgcc acgcctggca gtgagacttc ttttcattat tctgatgaat aatgtggact
     2461 agccgggcgc ggagccggtt tcaatcaacc tgcttgcgga agcgccacat cccgagcgcg
     2521 aagatgagca tgcccaggcc gagcatccag aggagctgcg gcaggacatc tgcgagctcg
     2581 gcgccccgga ggaagatcgc gcgctccgcc ttgaggaagt gcgtggtggc aatcgcgtag
     2641 gagagcgggc gcaggagggc cggcacgttc tccacgggga agatgaatcc ggaaagcagg
     2701 atcgaggggt tgaccagcag gaacgcggtg agctgcgcct cctgctgagt gcggctgatc
     2761 gtggagatga gcagcccggt gccaagcgtg ttcagcatca gcccgaggac gcagatgagg
     2821 acgagcccgg gcgagccgag catcggcacg cggaaccaga agcggatcac cgccaggatg
     2881 atcgccgcgt cgagaagggc gaggatgccc agcgggatca tcttgccggc caggagctcc
     2941 catccccgga ggggcgtgac cacgagctgc tccagcgtgc ccacctcgcg ctcgcgcacg
     3001 atggagaggc tggtcgcgat ggtcgtggtg accaccagga ccagggcgag cacgccgggc
     3061 accatgtagt gcgtgctgag gagctgcggg ttgaaccaga cgcgcggctg tgggctgacg
     3121 cctggccgcg ttccctcgtc ccgggggcgc ctcagcgtct cgatcatcag agacgagtgc
     3181 gccgcttgaa gatcgagcgc gaggtactcg gccgcgaggg tggcggaggt ggagtcggat
     3241 ccgtccacga gcacctgcac cgatggcgtc tcgccccgga ggatgcggcg cgtgaactcg
     3301 ggcgggatcg cgagcgccac ttcggcccgg cggtgagcca gcatccgggg gaggtcgcgc
     3361 gggttctcca ccgggtggat gatgaagtgc tggctggccg cgaccgcctg cacaagcgcg
     3421 cggctctgct gcgagcggtc gccgtcatac actgccagcg gcacgttgcg cacgtcgttg
     3481 gagacggcgt agccgacgat gatgagctgc agcaccgggg ccacggtgaa gagcgccagc
     3541 gtccggcggt cgcgccggag ctgctggaac tctttccgga tcatcgtgag cagtcgttcc
     3601 accggacctc cctcagttca tggtcacaac tgcttccgaa agcgccgggc gcacgccagc
     3661 aggaagaagg tagcgaaccc ggccaggatg agcgccgggc gccacagcac gtcccagcca
     3721 accgctttca agtagatccc ccggacaatc accaggtagt gcgtggcggg aaaaaggttc
     3781 gatagcg
//
LOCUS       DAOSCV010000395         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15347_length_3783_cov_3.324839, whole genome shotgun sequence.
ACCESSION   DAOSCV010000395 DAOSCV010000000
VERSION     DAOSCV010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15347_length_3783_cov_3.324839"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="PLY56_10350"
     CDS             <1..1297
                     /locus_tag="PLY56_10350"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ21926.1"
                     /translation="PEYASGRPRDLAWCRNNASHNVVTVDGAAQNSSYTGRLLSFEPE
                     GKARLADLSSDGIYPNCTTYRRTAWLVDASARYSYVLDLFRVRGGKEHTLSLHGPPGE
                     VTATGLVLKRQEKGTFAGPDVPFEALDKEWRSKAGFSFLYNVERMANPPATFTLDYRA
                     MDTRNRIAEGREPHLRIHSLTPAQEAALADGDPPQNKRGAPRRMRYLLLTRKGENLES
                     TFVTLLEPYDRQPFIQSVRRLEVVEAAPGTHPVAVEVTLADGRRDVLISCETPGRVRV
                     DGGITLEGAHGFLSLRDGQVECAKLIEGTLLERGAFRLTAETPAYTGTLARVLADDPN
                     DQQLELSAPLSNPKALAGRTIFVENDGKQGAAYTVVSAQGARVSLGAMSLVRGYQDPN
                     DYAKGYLYNVLPGQSYRIPTFAYVDLATGARIGSADSKP"
     gene            1315..2451
                     /locus_tag="PLY56_10355"
     CDS             1315..2451
                     /locus_tag="PLY56_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ21927.1"
                     /translation="MIDCDFPGGNILVEGIEGNTVRVRQDLRDTRGDWFYWYFRVRGA
                     AGCTLTFEFTQSNVIGVRGPAVSTDGGQTWCWLGSDAVRGSSFRYTFPADADEVRFGL
                     AMPYVQAHLDAFLRQHEGNPALRRDVLCRTPKGREVELLHVGRLDGKAPHKALLTCRH
                     HCCEMMASYALEGILTTILSDTEDGQWLREHVEFLVVPFVDKDGVEDGDQGKNRFPRD
                     HNRDYGPDSLYPTTRAIKELVPRWAEGRLRFTLDLHCPWIRGEYNEHIYFVGSENQEN
                     WQHVLGFARLLEQIQTGPLPYRVDGNLPFGQRWNTAANYHQGMSCARWAAGLPGIWAA
                     MSIEIPYANVLGAPVTPESARLFGQDLARTMRRWLDAQDGREQP"
     gene            2448..3458
                     /locus_tag="PLY56_10360"
     CDS             2448..3458
                     /locus_tag="PLY56_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOJ21928.1"
                     /translation="MKIPIPQIKAPEFWLANVDAVERYLTTQVVRGRMHIAGYSTHVR
                     PVRVLEYAVPHAASTLLVIGGTHGHEPGTVAAAMNLIHLLETGRDLAGKEHPTLLALL
                     QKTHLYICPMLNPDGRAVCPNTFYAQGLDTCTIYASGLQLDGSLVPYDADSSEPCYYF
                     DPEKCLFIGGQFNGAGWAINRRLSPDHSEAVEVQSLLEFVKGRGIEAILDLHACGYNF
                     AFQARTHPAPYWPVMREWQRRAEKLFASKGRPLGKLHGDGDPPVAPEFHFNSTLFHRH
                     AKLMWMAFEGRQGFLGRTSFMPLPTEWGIIDDYLSAITVFVELGSEGYYARANRQTFG
                     GG"
     gene            complement(3481..>3783)
                     /locus_tag="PLY56_10365"
     CDS             complement(3481..>3783)
                     /locus_tag="PLY56_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-beta-D-heptose 1-phosphate
                     adenosyltransferase"
                     /protein_id="HOJ21929.1"
                     /translation="LASLRVDILFIGCDGASAEEGFYTADLHLLSLERAMIAAAARVV
                     LLTESAKFGRRALARFATPDQVHTLVTDTGLVPRDRERLEEQGVRLLLTGSLRGRE"
BASE COUNT          654 a         1297 c         1252 g          580 t
ORIGIN      
        1 ccccgagtac gccagcggcc gcccgcgcga cctggcgtgg tgccgcaaca acgcttcgca
       61 caacgtggtc accgtggatg gcgccgcgca gaacagctcc tacaccggac gcctcctctc
      121 gttcgagccg gaaggcaagg cgcgcctggc agatctctct agcgatggca tctaccccaa
      181 ctgcaccacc taccggcgca ccgcctggct ggtggacgca agcgcgcgct atagctacgt
      241 gctcgatctc ttccgggtgc gcggcggcaa ggagcacacg ctctcgttgc acggccctcc
      301 cggggaggtg accgccaccg ggctcgtcct gaagcggcag gagaaaggca ccttcgctgg
      361 cccagacgtg ccgttcgagg cgctggacaa ggagtggcgc tcgaaggctg gcttctcgtt
      421 cctctataac gtcgagcgca tggccaaccc gccggccacg ttcacgctcg attaccgcgc
      481 catggacaca cgcaaccgga tcgcggaggg gcgcgagccg catctgcgga tccacagcct
      541 gacgcccgcg caggaggcgg cgctggcgga tggcgacccg ccccagaaca agcgcggcgc
      601 tccccggcgg atgcgctacc tcctcctcac gcgcaagggc gagaacctgg aatcgacctt
      661 cgtcacgctc ctggagccgt atgaccggca gccgttcatc cagagcgtgc gccggctgga
      721 ggtggtcgag gccgccccag gcacccaccc cgtggccgtg gaggtgacgc tcgccgatgg
      781 ccggcgcgat gtgctgatca gctgcgaaac cccgggccgc gttcgcgtgg acggcgggat
      841 cacactggag ggcgcccatg gcttcctctc gctgcgcgac ggccaggtgg agtgcgccaa
      901 gctgattgaa ggcacgctcc tggagcgcgg cgctttccgg ctgaccgccg aaacgcccgc
      961 gtacaccggc accctcgcgc gcgtgctcgc ggacgacccg aacgaccagc agctcgagct
     1021 gagcgcccct ctctcaaacc cgaaggcgct cgccgggcgc acgatcttcg tggagaacga
     1081 cggcaagcag ggcgccgcct acaccgttgt gtcggcccag ggcgcccgag tctcgctcgg
     1141 agctatgtcg ctggtgcgcg gctatcagga ccccaacgac tacgcgaaag gctacctcta
     1201 taacgtcctg ccggggcaga gctaccgcat cccaaccttc gcctatgtgg acctcgccac
     1261 cggcgcccgg atcgggagcg cggatagcaa gccgtgaagg gagatccaga tccgatgatc
     1321 gattgcgact tcccaggcgg aaatatcctc gtggaaggca tagagggaaa cacggttcgc
     1381 gtgcgccagg acctgcgcga cacgcggggc gactggtttt actggtattt ccgggtgcgc
     1441 ggggcagccg ggtgcactct caccttcgag ttcacccaaa gcaacgtgat tggcgtgcgc
     1501 ggtccggccg tgagcaccga tggcggccag acctggtgct ggctcggctc ggacgcggtg
     1561 cgcggctcat cgttccgcta caccttccct gccgatgccg atgaggtgcg cttcggcctc
     1621 gcgatgccct acgtccaggc tcacctagac gcgttcctgc gccagcacga gggcaaccct
     1681 gcgcttaggc gcgacgtcct ctgccgcacg cccaagggcc gggaggtgga gctactccac
     1741 gtgggacggc tggacggcaa agcgccacac aaggcgctcc tcacctgccg gcaccactgc
     1801 tgtgagatga tggcgagcta cgccctggag ggaatcctca ccaccatcct ctcggatacg
     1861 gaggacgggc agtggctgcg cgagcacgtc gagttcctcg tcgtgccatt cgtcgataag
     1921 gatggcgtgg aggacggcga ccagggaaag aaccgcttcc cgcgtgacca caaccgcgac
     1981 tacggcccgg atagcctcta ccccaccacg cgcgcgatca aagagctcgt gccgcgatgg
     2041 gccgagggcc ggctgcggtt cacgctggac ctgcactgcc cctggatccg cggcgagtat
     2101 aacgagcata tctacttcgt cggctccgag aaccaggaga actggcagca tgtgcttggc
     2161 ttcgcccggc tgctggagca gatccagacc ggaccgctcc cctaccgggt cgatgggaat
     2221 ctgcccttcg ggcagcgctg gaacacagcg gccaactacc atcaaggaat gagctgcgcg
     2281 cgctgggccg cgggtctccc tggcatctgg gcggcgatgt cgattgagat cccctatgcc
     2341 aacgtgctgg gagcaccggt caccccagag agcgcgcgcc tcttcgggca agatctcgcc
     2401 cggacaatgc gccgctggct cgatgcccag gatgggaggg aacagccgtg aagataccga
     2461 tcccccagat caaggcgccg gaattctggc tggcgaacgt ggatgccgtc gaacgctacc
     2521 tgacgaccca ggtggtccgg ggccggatgc acatcgccgg ctactcgacg cacgtccggc
     2581 cggtgcgcgt gttggagtat gctgtgcccc atgccgcctc gacgctgctc gtcattggtg
     2641 gcacccacgg gcacgagccc ggaacggtcg ccgcggcgat gaacctgatc cacctgctgg
     2701 agacggggcg cgacctggct ggcaaggagc accccaccct cctcgcgctc ctccagaaga
     2761 cgcacctcta catctgcccg atgctcaacc cggatgggcg cgccgtctgc ccgaacactt
     2821 tttatgcgca gggcctggac acatgcacga tctacgcctc cgggctgcag ctggatggga
     2881 gcctggtgcc ctacgacgcg gacagcagcg agccgtgcta ctacttcgac ccggagaagt
     2941 gcctcttcat cggcgggcag ttcaacggcg cagggtgggc aatcaaccgc cggctctcgc
     3001 cggaccatag cgaggcggtg gaggtgcagt cgctcctgga gttcgtgaag ggccggggca
     3061 tcgaggcgat cctcgaccta cacgcctgcg gctacaactt cgctttccag gcgcgcacgc
     3121 acccggcacc ctactggccg gtgatgcgag agtggcagcg ccgggccgag aagctgtttg
     3181 ccagcaaggg ccggccgctc ggcaagctac acggcgatgg cgacccgccg gtcgccccgg
     3241 agttccactt taactccaca ctcttccaca ggcacgcgaa gctgatgtgg atggcgtttg
     3301 agggccggca gggcttcctc gggcggacga gcttcatgcc tctccccacc gagtggggga
     3361 tcatcgacga ttatctctcc gcgatcaccg tcttcgtgga gctggggagc gagggctact
     3421 acgcccgggc caaccgccag actttcggtg ggggatagcc cggcagggcg caggtgtgga
     3481 tcactcgcgc ccgcgcaggg aaccggtcag gagcaggcgc accccctgct cttccagccg
     3541 ctcacggtcc cggggcacca ggccggtatc agtcacgagg gtgtgcacct ggtcgggggt
     3601 ggcgaagcgc gccagggcac gccggccaaa cttcgcgctc tcggtcagca gaacgacgcg
     3661 agcggcggcg gcgatcatcg cccgctcgag gctgaggagg tggaggtcgg cggtgtagaa
     3721 cccctcttcc gcgctggcgc catcgcaccc gatgaagagg atatccacgc gcaggctggc
     3781 aag
//
LOCUS       DAOSCV010000396         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15395_length_3774_cov_3.343372, whole genome shotgun sequence.
ACCESSION   DAOSCV010000396 DAOSCV010000000
VERSION     DAOSCV010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15395_length_3774_cov_3.343372"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLY56_10370"
     CDS             <1..692
                     /locus_tag="PLY56_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ21930.1"
                     /translation="NLQGVRERIARAASRVGRSPDEVTLVVVTKTWPTEVVREAIAAG
                     AEHIGENYVQEAREKRAQIPDPVTWHLIGHLQSNKAGQAAALFDIIHTVDSLHLAERL
                     SRHAVAAGRNLEVLVEVNISGEASKFGVAPEEAGALMDALGALPGIRPSGLMGMPPFS
                     ADPEEARPYFRRLRALWEALPPAYRRHLSMGMSGDFEVAVEEGATLVRVGSAIFGPRR
                     ARATGTHDRQP"
     gene            723..1181
                     /locus_tag="PLY56_10375"
     CDS             723..1181
                     /locus_tag="PLY56_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOJ21931.1"
                     /translation="MTKRFTLEDEEIEETQPGRWTRFKEFIGLGTYEDEEEEEPEPTP
                     SRRKGQILRLQSGRTHQIHKICIRSLEDARYAADMLKERRPVILNLEQSDEEMARRAI
                     DFVSGATYALDGYFERVGERVFLFTPSNVLIATDENSEFSSSQGLYMNAQ"
     gene            1183..2001
                     /gene="proC"
                     /locus_tag="PLY56_10380"
     CDS             1183..2001
                     /gene="proC"
                     /locus_tag="PLY56_10380"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ21932.1"
                     /translation="MSNTRLACIGSGFMGEALIRGILAAGIYAPDEVCVSDISTARLA
                     VLATLGVRTTQENAEAVAGAETVLLAVKPQVIGQVLEGLRPHLSSDTLVLSIAAGVSL
                     ATLEGKLAPGTPVVRAMPNLLATVRAAATALAPGSAAGAEHLERAERIFGAVGTAVRV
                     EERLLNAVTGVSGSGPAYLCLVIEALADAGVQAGLTREVAQQLAAQTVMGTGKLILES
                     GQHPAEVKDRVSSPGGTTIAGIRALERAGLRAALMDAVDAAVTRAAELERAAAK"
     gene            2035..2316
                     /locus_tag="PLY56_10385"
     CDS             2035..2316
                     /locus_tag="PLY56_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOJ21933.1"
                     /translation="MEEVKRDMALAQMVKLFFDALWLLIIIYVLGSWVPSLQRQSWFR
                     TLRGVVEPMLVPFRRIIPPENLGGLDLSPVFLMLALSLVQQLLLRALIG"
     gene            2411..2875
                     /locus_tag="PLY56_10390"
     CDS             2411..2875
                     /locus_tag="PLY56_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOJ21934.1"
                     /translation="MRLAPLDIYNKEFSRAFSGYHRGEVDSFLEEVARDYEALTRENV
                     ELRQQVEEMGKRLADYARLEESIRKAMVLAQSSADEARANARRESELIIQEARQEAAR
                     IQEQAHALVRQEEERGAKLREQRERFVLEFTALLRSHLAALENGTREDGESP"
     gene            2859..>3774
                     /locus_tag="PLY56_10395"
     CDS             2859..>3774
                     /locus_tag="PLY56_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21935.1"
                     /translation="MVNPLRTALFGLLFVASLFLLAVNAREQHRQAAVAGDPLEAINA
                     DLVARIAIVHLRDPALPTSDPLARRALRTAIQRYRVLLRAHPRSPVLQQRIGILLGVN
                     EGPEAAWEWLRARSSGPDLMLWQAAYAPGALSPAARDAALSRLEALNLAWYGELVRAR
                     IYAKAGAASQAQETLARLQRRLRPGVMGAILFSSLALIAVPFGAGVWVAALLLRQQGL
                     LRPADRPQPFTAVRPRALIEAFVAWFFAIIAGGWGLRQLLAALDLPREWPLAAPIGLL
                     LLTQIVAAAAGGALLLAGARRQGSGEAPE"
BASE COUNT          612 a         1239 c         1266 g          657 t
ORIGIN      
        1 gaaatcttca gggggtgcga gaacgcatcg cccgggctgc gagccgggtg ggccggtcgc
       61 ctgacgaggt aaccctcgtg gtggtgacca agacgtggcc gacagaggtc gtccgagagg
      121 cgatcgccgc tggcgcggaa cacatcggcg agaactacgt gcaagaggcc cgggagaagc
      181 gcgctcagat tcccgaccca gtgacctggc acctgatcgg acacctccag tccaacaagg
      241 caggccaggc cgctgctctc ttcgacatca ttcacaccgt agacagcctg catctcgccg
      301 agcgtctctc gcgccatgca gtggcagcgg ggcgcaattt agaagtgctc gtggaggtca
      361 acatctccgg cgaagcgagc aaatttggtg tcgcgccgga agaagcaggt gcgcttatgg
      421 atgccctggg tgccctgcca ggcatccgcc catccgggtt gatgggcatg ccgcctttct
      481 ccgcggaccc cgaagaagcc cgaccctact tccgtcgtct ccgggcgctg tgggaggcgc
      541 tgccccccgc gtatcgccgc catctgtcga tgggcatgag cggtgacttt gaagtggcgg
      601 tggaagaggg agccaccctg gtccgggtgg gcagtgcgat ttttggacca cggcgggcgc
      661 gagccacggg cacgcacgac cgccagccat aacctgttcc cacacggagg gagtagccca
      721 cgatgaccaa acgctttacg ctcgaggacg aggagattga ggagacccag ccggggcgtt
      781 ggactcggtt caaggagttc atcggactgg gcacctatga ggacgaggaa gaggaagagc
      841 cagagcctac cccctccagg cgaaaggggc aaatcctccg tctccagtct gggcgaacgc
      901 accagattca caagatttgc atccggtcgc tcgaggatgc gcgctatgcc gccgacatgc
      961 ttaaggaacg ccggcccgtg atcctcaacc tggagcagtc ggatgaggag atggcgcggc
     1021 gtgccatcga cttcgtcagc ggcgccacct atgccctcga tggatacttc gagcgcgtcg
     1081 gcgagcgggt tttcctcttc acgcccagca acgttctcat cgccaccgat gagaactcgg
     1141 agttcagctc ctcgcagggc ctctacatga atgcgcaatg agatgagcaa cacacggctg
     1201 gcctgcatcg gctccgggtt tatgggcgag gcgctcatcc gcggcatcct ggccgcgggt
     1261 atctacgccc ctgacgaggt ctgcgtcagc gacatcagca ccgcgcgcct ggccgtcctc
     1321 gccactctgg gagtgcgcac cactcaggag aatgccgagg cggtggcggg cgcggagacc
     1381 gttctcctgg ctgtcaagcc ccaggtgatc ggtcaggtgc tcgaggggct gcgccctcat
     1441 ctttcttccg atacgctggt cctctctatc gccgccggcg tttcgctcgc cactctggag
     1501 ggtaagttag ccccaggcac ccccgtggtg cgcgcgatgc ccaatttgct ggcaaccgtc
     1561 cgcgcggcgg caacggcgct cgctcccggt tcggccgctg gcgcggaaca tctggagcgc
     1621 gccgagagga ttttcggtgc cgttggcacc gccgtgcgcg tcgaggagag actgctcaac
     1681 gcggtcaccg gggtcagcgg gagcggcccg gcctatctct gcctggtgat cgaggcgctt
     1741 gccgatgcgg gcgttcaggc cgggctgacg cgagaggtcg cacaacagct ggccgcccag
     1801 accgtgatgg gcacgggaaa gctgattctc gaatcagggc agcaccccgc cgaggtgaag
     1861 gaccgggtct cgtccccggg tggcaccacc atcgccggga ttcgggcgct ggagcgcgct
     1921 gggctgcgtg ccgcgctgat ggacgcggtg gatgccgcgg tcacgcgagc cgccgagttg
     1981 gaacgtgctg cggcgaaata gcgtcctcca tctcatgcag ttgttgcctg cgaattggag
     2041 gaggtcaagc gcgatatggc tttagcccag atggtgaagc tctttttcga cgcgctgtgg
     2101 ctgctcatca tcatctatgt gctgggatcg tgggttccta gcctgcagcg tcaatcgtgg
     2161 ttccgcaccc tccggggggt cgtggagcca atgctcgtgc cgttccgccg gatcatcccg
     2221 ccggagaacc tgggcgggct cgacctgtcc ccggttttcc tgatgctcgc gctcagcctc
     2281 gtgcagcagc tgctgctgcg cgcgctgatc ggctgatgtt caggcatcgc gcgggagaag
     2341 caccggcact accaggatgc ggccggtcgt atccagccgc ggtggaaagg atcaacctga
     2401 agggaactag atgcgccttg ccccgctaga tatctacaac aaagagtttt cccgcgcttt
     2461 cagtggctac catcggggcg aggttgattc gttcctggag gaagtggccc gcgactacga
     2521 agccctcact cgcgagaatg tggagctgcg ccagcaagtc gaggagatgg gcaagcgcct
     2581 ggcagactat gcccgcctgg aggagagcat ccgcaaggcg atggtcctcg cccaaagcag
     2641 tgcagacgaa gcccgggcca acgcgcgccg cgaaagtgag ttgatcatcc aggaagcgcg
     2701 ccaggaagcg gcgcggattc aggagcaggc gcacgcgctc gttcgccagg aagaggagcg
     2761 gggcgcgaaa ctacgcgagc agcgtgagcg cttcgtcctc gagttcaccg cgctcctccg
     2821 gtctcacctc gccgcgttgg agaacggcac gcgggaagat ggtgaatccc cttaggactg
     2881 ctctcttcgg gctcctgttc gtggcgtcgc ttttcctcct cgccgtgaac gcgcgcgagc
     2941 agcaccgtca ggccgcggtg gccggcgacc ccctcgaagc aatcaacgct gaccttgtcg
     3001 cgcgcatcgc catcgtccac ctgcgcgatc ccgcgctccc caccagcgat cctctggccc
     3061 gccgcgcgct gcgcaccgcg atccaacgct atcgcgtgct cctccgggcc catccgcgct
     3121 ccccggttct ccagcagcgc atcggcattc tcctgggcgt gaacgagggt ccggaagccg
     3181 cctgggaatg gcttcgggcg cgctcgtcgg ggccagacct catgctatgg caagccgcgt
     3241 atgctcccgg cgcgctctca ccggcggcgc gcgacgcggc actctcgcgc cttgaagccc
     3301 tgaatctggc ctggtatggc gagttggtgc gcgcgcggat ctacgcgaag gcaggggccg
     3361 cgtcgcaagc ccaggagacg ctggcgcgcc tgcagcggcg cttgcgccct ggggtgatgg
     3421 gtgctatcct cttctcttcc ctggcgctca tcgcggtacc gtttggcgcc ggagtgtggg
     3481 tagcggcgct gctcctccgc cagcaggggc tcctccgccc cgcggatagg ccgcagccct
     3541 tcacggcggt gaggccgcgc gctctcatcg aagcctttgt cgcgtggttt ttcgcgatta
     3601 tcgcgggcgg ctggggcctc cggcagctgc tggcggctct ggatctgccg cgcgagtggc
     3661 cgctggcggc gcccatcggc ctccttctac tcacccagat cgtcgcggcg gccgcgggcg
     3721 gtgccctcct cctcgcgggc gcgcgccgcc agggctccgg cgaagccccc gaga
//
LOCUS       DAOSCV010000397         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15398_length_3774_cov_2.542888, whole genome shotgun sequence.
ACCESSION   DAOSCV010000397 DAOSCV010000000
VERSION     DAOSCV010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15398_length_3774_cov_2.542888"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="PLY56_10400"
     CDS             complement(<1..1048)
                     /locus_tag="PLY56_10400"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ21936.1"
                     /translation="MTTRMQAVRDEFARVFGGECTLVRAPGRVNLIGEHTDYNEGFVF
                     PVAIDREVRFAVSPRDDRKVVLRSLNFDAESEFSLSNITKDADQPWSNYVRGIAAVLQ
                     DAGHVLRGMNAVIEGNVPLGSGLSSSAAMEIGALLAFEAVSGFQIEPVSAARLAQKAE
                     NQFVGVNCGIMDQFISRLGRKGHALLIDCRTLSYEPVPVGGDEVRIVVADSKVKRGLV
                     DSEYNKRRAECERAVVLLSGRLSNIGALRDVTPEQLAAHGDLLADVTRRRARHVVTEN
                     DRCLRSVEALRAGDLEAFGKLMNQSHISLRDDYEVSCRELDILVEAAWAVPGVLGSRM
                     TGAGFGGCTVSLVRA"
     gene            complement(1148..3187)
                     /locus_tag="PLY56_10405"
     CDS             complement(1148..3187)
                     /locus_tag="PLY56_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ21937.1"
                     /translation="MRIYGLRILMGPCVRGVACLLGLAAWLLPGAAMAQVQRNEAAIR
                     DVRLGKIQEAKASWWGFDPDDSTRMLQEAINSGARKLIVDNVGKPWIVTGIALASNQE
                     ILFERGVEVLAKKGAFKGRNDSLFYADLKENITLTGYGATLRMRRADYDGPEYEKAEW
                     RHCLSFRSCSNIKVYGLTLAESGGDGIYLGTAKSGVTNKDVHIKDVVCDRNYRQGISV
                     ITAENLLIENTVMKDTAGTAPQAGIDFEPNHPSERLVNCVMRNCITQNNKGHGYVLYL
                     PPLKATSEPVSVRFENCRAIGDQLSSACVVTANTPDGAVKGTIEFINCHFEASKGAGI
                     NVSDKPANGCRVRFEKCTVVNPAAEAANQAPIVLMARQGAGDPIGGVEFDRCVIRDSV
                     DRVPMKYVDSAGGVGIEQVTGTLLLEKDGKRQETPLTKELLAQWMPIMRLRVLPRFEM
                     KGVTFKPLEETVPAEKRNFASARQRRGGNYVLYARQGDEVSLRFTYGQVGRYSGRTMP
                     VMVTGPSGQEVQRAQVAFKEEGEVRFTAPETGLYRVKADPGGNYVRLLESSHAVSLSG
                     DGQSIRLYISPGDYYFWVPEGVSEFAVRVFGEGLGEAVKATLLDPAGKVVQEVDNAAQ
                     THQFEVKLAKPSAGEAWCLRLEKPTQLAMEDHYVDLRVVPPFLAPSKEALLIPVK"
     gene            complement(3295..>3774)
                     /locus_tag="PLY56_10410"
     CDS             complement(3295..>3774)
                     /locus_tag="PLY56_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21938.1"
                     /translation="ETEPKIPCTINAYFNDSGRAGLTIELSGQYVVLEKEVRRGLRAA
                     LNIARFLEMLPGTPEGLDEPTVWLDRATVVEVRAPASGLFVEAGLETCDWVEAGQPLG
                     HLLSDVDLSVTPILAPVSGRLRGYGCHRMHADVALPAMHPYASEGDSLAVIAAPAGE"
BASE COUNT          659 a         1333 c         1121 g          661 t
ORIGIN      
        1 ccgcgcgcac caggctgacg gtgcatcccc caaaaccggc accggtcatg cgcgatccga
       61 ggacgcccgg caccgcccag gccgcctcga cgaggatgtc cagctcgcgg cagctcacct
      121 cgtaatcatc gcgcaggctg atgtgcgact gattcatcag cttcccgaac gcctccaggt
      181 cgcccgcgcg aagggcctcg acgctgcgca ggcagcggtc gttctccgtc accacgtgcc
      241 gcgcgcgccg gcgcgtgacg tctgccagaa ggtctccgtg cgccgccagt tgctcaggag
      301 tgacgtcgcg tagggcaccg atgttggaga gccgcccgga gaggagcacc accgcgcgct
      361 cgcactcggc gcgccgcttg ttgtattctg aatcgaccag cccccgcttc accttggagt
      421 cggcgacgac gatgcgcacc tcatctccgc cgacgggcac cggctcgtag ctgagcgtgc
      481 ggcagtcaat caggagcgcg tgccctttcc ttccaagacg ggagataaac tggtccatga
      541 tgccgcagtt gacgcccacg aattgattct cggccttttg cgccagccgg gccgcggaga
      601 caggctcgat ctggaagccg gagaccgcct cgaacgcgag cagcgccccg atctccatcg
      661 cggcggaaga gctgagcccc gagccgaggg gcacattccc ctcgatcacg gcgttcatcc
      721 cacgcagcac gtgccccgca tcctggagca cggcggcgat cccgcgcacg tagttgctcc
      781 agggctgatc cgcgtctttc gtgatgttgg agagcgagaa ttccgattcg gcatcgaagt
      841 tgagcgaccg gaggaccacc ttgcggtcat cccgcgggct cacggcaaag cgaacctcgc
      901 ggtcgatggc aacggggaag acaaaaccct cgttatagtc ggtatgctcg ccgatgaggt
      961 tgacgcgacc gggcgcccgc accagcgtgc actcgccacc gaacacgcgc gcgaactcgt
     1021 ctcgaactgc ttgcatccgc gttgtcatca atcaggcctc cagaccggca tcatacttcg
     1081 atggagatag gaaccccctc cactcccaga gtcagttcgc gggagtggag ggggttcctg
     1141 cccgctctca cttcaccggg atgagtagcg cctccttcga gggggcgagg aaaggcggca
     1201 ccacccgcag atcgacgtag tggtcctcca tcgccagctg cgttggcttc tccaggcgga
     1261 ggcaccacgc ctcacccgcc gagggcttcg ccagcttcac ctcgaactga tgcgtctgcg
     1321 ccgcgttatc cacctcttgg acgaccttgc cggccggatc cagcagcgtg gccttcaccg
     1381 cctcgccgag gccctcgccg aagacgcgaa ccgcgaactc cgacacgccc tccggcaccc
     1441 agaagtagta atcgcccggc gaaatgtaga ggcgaatcga ctgaccatcg ccgctgaggc
     1501 tcaccgcgtg cgagctctcc agcaggcgca cgtagttgcc accgggatcg gctttcacgc
     1561 ggtagaggcc ggtctccggc gccgtgaagc gcacctcacc ctcctccttg aacgccacct
     1621 gggcgcgctg gacttcctgg cccgagggcc ccgtcaccat gaccggcatc gtcctgccgg
     1681 agtatctccc gacctgcccg taggtgaagc ggaggctcac ctcgtcgccc tggcgggcat
     1741 acagcacata gttgccaccc cggcgctggc gagccgaagc gaagttgcgc ttttcggccg
     1801 gcacagtctc ctcaagcggc ttgaaggtga cccccttcat ctcgaagcgc gggaggaccc
     1861 gaagccgcat gatcggcatc cactgggcca gcagctcctt cgtaagcggc gtctcctggc
     1921 gcttcccgtc cttctccaag agaagcgttc ccgtcacctg ctcgatgccc acgccgccag
     1981 ccgaatcgac atacttcatc ggaacgcggt cgaccgaatc gcggatcacg cacctgtcaa
     2041 actccacgcc gccaatcggg tcgccagcgc cctggcgggc cattaggaca ataggcgcct
     2101 ggttggccgc ttccgccgca gggttcacga cggtgcactt ctcgaagcga acccggcagc
     2161 cattcgcagg cttgtcagac acgttaattc ccgcgccctt gctcgcctcg aagtggcagt
     2221 tgatgaactc gatcgtcccc ttcacggcgc catcaggagt gttcgcggtg accacgcacg
     2281 cagacgagag ctggtcgcca atcgccctgc agttctcgaa gcggaccgaa accggctccg
     2341 aggttgcctt caggggcggt aggtagagga cgtagccatg ccccttattg ttctgggtga
     2401 tgcagttgcg catcacacag ttcaccaggc gctcactggg atggttgggc tcgaaatcga
     2461 tccccgcctg cggcgcggtg ccagccgtgt ccttcatcac cgtattctcg atcaagaggt
     2521 tctcggccgt gatcacgctg atcccctggc gatagttccg gtcgcacacc acatccttga
     2581 tatgcacgtc cttgttcgtg acgccggact ttgccgtgcc caggtagatg ccatcgccgc
     2641 cgctttcggc cagtgtcagg ccgtacacct tgatattgct gcagctccgg aagctgaggc
     2701 agtggcgcca ctccgccttt tcatactccg ggccgtcata atccgcccgg cgcatccgca
     2761 gcgtcgcgcc atagccggtc agggtgatgt tctctttcag gtccgcgtag aaaagcgagt
     2821 cgttcctccc cttgaaagcg cccttcttgg cgaggacctc cacgcctctc tcgaagagga
     2881 tctcctggtt gcttgccagg gcgatccccg tcacgatcca cggcttgccc acgttgtcca
     2941 cgatcagctt gcgcgcgccc gagttgatcg cctcttgcag catccgggtg gaatcgtccg
     3001 ggtcgaagcc ccaccacgag gctttcgcct cctggatctt gccgaggcga acgtcgcgga
     3061 tcgccgcttc attccgctgt acctgcgcca tggcggcccc cggaagaagc cacgccgcca
     3121 ggcccagcag acaggccaca ccgcggacgc atggccccat cagaatccgc agaccataga
     3181 tcctcatcct catcttctcc tttcggcagg tgagcacatg cctccggatc ggccccggcc
     3241 ggctcgctca cacccaggat acgccaatca ggcccgagaa ccttccccga cacctcactc
     3301 cccggcaggg gcagcaataa ccgccaggct gtcgccttca ctcgcgtagg ggtgcatcgc
     3361 cgggagagcc acatcggcgt gcatccggtg gcagccgtag ccgcggagac ggccgctcac
     3421 cggagccagg atgggcgtca cgctgagatc cacgtccgag agcaggtgtc ccagtggctg
     3481 gccggcttcc acccagtcgc acgtctccag cccagcctcg acaaagagcc ccgaggctgg
     3541 ggcgcgcacc tcgacaacgg tcgctcgatc cagccagacg gtcggctcgt ccaggccctc
     3601 gggcgtgccc gggagcatct cgaggaagcg cgcgatgttc aaggcggcgc gcaggccacg
     3661 gcggacttcc ttttccaaga cgacatactg gccagaaagc tcgatggtca gaccggctcg
     3721 cccgctgtca ttgaagtagg cattgatcgt gcagggtatc ttgggctcgg tctc
//
LOCUS       DAOSCV010000398         3771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15417_length_3771_cov_3.232508, whole genome shotgun sequence.
ACCESSION   DAOSCV010000398 DAOSCV010000000
VERSION     DAOSCV010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15417_length_3771_cov_3.232508"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLY56_10415"
     CDS             <1..1199
                     /locus_tag="PLY56_10415"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21939.1"
                     /translation="SSRAAQLMAQARASGVPGLALAAPTAYRASNLGVERATVLHALA
                     LAGEGGPIRLVLGGQTGARATRESGTLARLALAARRAGLVAEAESLCRRLVARSSESA
                     NFCHWNSDAETTALALQAILAVPGEPRDERMAVAAKAVRWLLSRREDGYWGSTQATAR
                     VLFALVEYLEATGETAPAFTVTPAVNGQALEPVRFDARSLFQPEVTITLPASALRAGE
                     NQITLRKQGAGSLYYTVRAREFVTQEEIPVLVGGAGIAVTRTYHRLRSDRDPKDGTLR
                     LMPSPRPVEHLRAGETYLSRLTIRSPRRYEHVVIEEPLPAGCEPLVRERTGEGEWNRW
                     WTHVETRDHQIAFFARQLDAGERVLEYHFRASVPGEYHVMPAVIQGMYDPEVRAGGAE
                     TRVEVR"
     gene            1196..1921
                     /locus_tag="PLY56_10420"
     CDS             1196..1921
                     /locus_tag="PLY56_10420"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ21940.1"
                     /translation="MRRRMALGLLSAGCLLLVAALGWAGARGAGTECVLGSYGTSLRG
                     RTRAQVFNALRAAQSLDGAVLQPGETFSFNKRVGPWNAAAGYRKAPVSFDGEMVPSYG
                     GGVCQTSTTLYNAAMLAGLEIVERHRHVRPPAYVAPGRDAAVAMPGVDLRIRNPYPYP
                     VRIQARNIGESLIVTVHGRGPRRPTVLRTEVESVARPLRVREAGMWKRGTPGFTALTY
                     RQAGAHAEVSSHLSYPPVNGLEP"
     gene            complement(1984..3540)
                     /locus_tag="PLY56_10425"
     CDS             complement(1984..3540)
                     /locus_tag="PLY56_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ21941.1"
                     /translation="MARRAYLGVLGILLLGPSPEARAAAEPANVAAAARVTAIRFTGV
                     TKLPEQRLLSLLRTRTGAPFDVETFEQDLERVETFLHENGFPLAYIEAGAGIDSEGVV
                     TIPIQEGRIEKVTITGTKRTRRGVILRELGVRAGDVYDARGLADDRQRLYRLGFFNSV
                     AIEPKPGSEPGKAILNIDVKERYTGSISAVMGYASNAGMVGYVQWSDTNFLGTGQTLY
                     VNWQRGTYSTVAYLTEHDLASRLLARTGYDIGYYTPWLLSRRLAAGLRYFNTGAYQFS
                     FFSEDIDTENIKSYEWRRGTWATLVGELGRDTSFGVLLRQENVDYNTAPVDLFAEAGA
                     LTTPARVSSVRLNLSRGPSHPFTLLDAVPRDGLFIELGHSRAAGASGAFQKFGLTKTQ
                     YVKLTPKNTLAARVQLGWSAGNLPFAELYSLGGPNTLRSYSYSRFLGTRMLALTTEWR
                     HQLTSAVQATAFLDLGNAWGGDQRFETGGLRSAIGLGFRLATPFGPLRLDYAFGGDGG
                     RFHVTTGSNF"
     gene            complement(3555..>3771)
                     /locus_tag="PLY56_10430"
     CDS             complement(3555..>3771)
                     /locus_tag="PLY56_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21942.1"
                     /translation="EHLGRLLQRDLQMRDVRVAPGGTAVTVAGETLLTVTEADAALQG
                     KTPAEVASEAARALSRVLWRVHVDRVS"
BASE COUNT          564 a         1291 c         1344 g          572 t
ORIGIN      
        1 cctcctcccg cgcggcccag ctgatggcgc aggcgcgcgc gtccggcgtg cccgggctgg
       61 cgctggccgc gcccacggca taccgagcat cgaaccttgg cgtggagcgg gcgaccgtgc
      121 tgcacgcgct tgccctcgcg ggcgagggtg gcccaattcg cctggtgttg ggggggcaaa
      181 ctggcgctcg tgccacgcgc gagagcggta cgctcgcccg gctcgcgctc gcggcgcgcc
      241 gagcagggct ggtggctgaa gccgagagcc tctgccggcg gctcgtcgcg cgatccagtg
      301 agagcgccaa cttctgccat tggaacagtg acgccgagac gacggcgctc gcgctccagg
      361 cgatcctggc cgtgccggga gagccgcgcg acgagcgcat ggcggtcgcc gcgaaggcgg
      421 tgcgctggct cctctcgcgc cgcgaggacg gctattgggg ctcgacccag gccaccgcgc
      481 gcgtcctctt cgccctggtg gagtatctcg aggcgacggg cgagaccgca ccggcgttca
      541 ccgtcactcc ggccgttaac gggcaggcgc tcgaaccggt acgcttcgac gcgcgctcgc
      601 tcttccagcc ggaggtgacc attacgctgc ccgcaagcgc gttgcgcgcg ggcgagaacc
      661 agatcacgct gcgcaagcaa ggcgcgggca gcctctacta cacggtgcgc gcgagagagt
      721 tcgtcacgca ggaggagatc cccgtgctgg tcggtggcgc cgggatcgcg gtgacgcgca
      781 cctaccaccg gctccgctcg gatcgcgacc ccaaggatgg cacgctccgg ctgatgcctt
      841 cgccgcggcc ggtcgaacac ctccgcgctg gcgagaccta cctgagccgg ctgacgatcc
      901 gctcgccgcg gcgctatgag cacgtcgtga tcgaggagcc gctgccggct ggatgcgagc
      961 cgctggtccg ggagcgcacc ggcgagggtg agtggaaccg gtggtggacc cacgtggaga
     1021 cgcgcgacca ccagatcgcc ttcttcgccc ggcagctcga cgcgggcgag cgcgtgctgg
     1081 agtatcactt ccgcgcctcg gtgcctgggg agtatcatgt gatgcctgcc gtgatccagg
     1141 ggatgtacga cccggaggtg cgcgccggcg gcgcggagac acgggtggag gtgcgatgag
     1201 acggcgcatg gccctggggc tcctttccgc ggggtgccta ctcctggtgg cggcgctggg
     1261 atgggccggg gcgcgcggcg cgggcaccga gtgcgtgctc ggcagctacg gcaccagcct
     1321 tcgcgggcgc acgcgcgcgc aggtgttcaa cgccctgcgc gccgcgcaga gcctggatgg
     1381 cgccgtgctc cagccgggtg agacgttctc ctttaacaag cgcgtgggcc cctggaacgc
     1441 cgccgccggc taccggaagg cgcccgtcag cttcgatggc gagatggtgc cctcgtacgg
     1501 cggtggggtg tgccagacct ccaccacgct ctacaacgcc gcgatgctgg ccggcctgga
     1561 gatcgtggag cgacaccggc acgtccgccc gccggcctac gtggcgcccg ggcgcgatgc
     1621 cgcggtcgcc atgccgggcg tcgatctgcg gatccgcaac ccctatccct acccggtgcg
     1681 catccaggcc cggaacatcg gcgagagcct gatcgtgacg gtgcatggcc ggggcccgcg
     1741 ccggcccacc gtcctccgca ccgaggtgga gagcgtcgcc cggcccttgc gcgtgcgcga
     1801 agcggggatg tggaagcgcg ggaccccggg attcacggcg ctcacctacc gccaggcagg
     1861 cgcgcacgcg gaggtctcat cccacctctc ttacccgccg gtaaatggcc tggaaccgta
     1921 gccgcggcag cgcgcgccgg gatggcgtgg ggggcggggc cggtgatggc cccgcctgcc
     1981 cgactagaag ttgctgccgg tggtgacgtg gaagcgcccg ccgtcgccac cgaaggcgta
     2041 atcgaggcgc agggggccga acggcgtcgc caggcggaag ccgaggccaa tcgccgaacg
     2101 aaggccgccc gtctcgaaac gctggtcgcc tccccaggcg ttgccgaggt ccaggaaagc
     2161 ggtggcctgc acggcgctcg tcagctgatg gcgccactcg gtggtgaggg cgagcatccg
     2221 cgttccgagg aagcggctat agctatagga gcgcagcgtg ttcggcccgc cgagtgagta
     2281 aagctccgcg aagggcaggt ttccagccga ccagccgagc tgcacccgcg ccgccagggt
     2341 gttcttcggt gtcagcttta catactgcgt cttcgtcagc ccgaacttct ggaaagcgcc
     2401 ggatgcgccc gcggcccttg agtgccccag ctcgatgaag aggccgtcgc gcggcacagc
     2461 atcgagcagg gtgaaggggt gtgatggacc gcgcgataga ttgagcctca cgctggagac
     2521 gcgggccggc gtggtcagcg cgccggcctc ggcgaagaga tcgactggcg ccgtgttgta
     2581 gtccacattc tcctgccgca gcagaacccc aaaggatgtg tctctcccca gctccccgac
     2641 gagcgtggcc caggtgcccc ggcgccactc gtagctcttg atgttctccg tgtcgatatc
     2701 ctcgctgaag aaggagaact ggtacgctcc ggtgttgaag tagcgcagcc ctgccgcgag
     2761 cctccggctg agcagccagg gggtgtagta gccgatgtca taccccgtcc gcgccaggag
     2821 ccgggatgcg aggtcgtgct ccgtcaggta ggcgacggtg gagtaggtgc cgcgctgcca
     2881 gttgacgtag agcgtctgcc ccgttccgag gaagttcgtg tcgctccact gcacgtagcc
     2941 caccatgccg gcgttggatg cgtagcccat cacggcggag atggagccgg tgtagcgctc
     3001 cttgacatcg atgttgagga tcgccttccc cggctcggag cccggctttg gttcgatggc
     3061 gacgctgttg aagaagccga ggcggtagag ccgctgccgg tcatcggcca gcccgcgggc
     3121 atcgtagaca tcccccgcgc gcacccccaa ttcgcggagg atcacccccc gccgggtccg
     3181 cttcgtgccg gtgatggtca ccttctcgat ccggccctcc tggatcggga tggtgacgac
     3241 gccctcgcta tcgatgccgg caccagcttc gatgtaggcc aggggaaagc cgttctcatg
     3301 cagaaacgtc tccacccgct ccaggtcttg ctcgaatgtc tccacgtcga atggggcgcc
     3361 ggtgcgcgtt cgcagcagcg acagcagccg ctgctcgggc agcttcgtca ccccggtgaa
     3421 tcggatcgcg gtgactcgcg ctgccgcggc gacattggcc ggctcagcgg ccgctcgtgc
     3481 ctccggggaa ggccccagaa gaaggatccc caatactccc aggtacgctc gtcgtgccaa
     3541 cgtccccctc cacatcacga cacccgatcc acgtgaacgc gccagagcac ccgagaaagg
     3601 gcgcgagcgg cctccgaggc aacctccgca ggcgtcttgc cttgcagggc ggcgtcggcc
     3661 tcggtgacgg tcaacagggt ttcacccgcg actgtgacgg ctgtgccgcc gggggcgacg
     3721 cggacatcac gcatctgaag gtcgcgctgc aggagccggc cgaggtgctc c
//
LOCUS       DAOSCV010000399         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15423_length_3770_cov_3.096904, whole genome shotgun sequence.
ACCESSION   DAOSCV010000399 DAOSCV010000000
VERSION     DAOSCV010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15423_length_3770_cov_3.096904"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..528
                     /locus_tag="PLY56_10435"
     CDS             76..528
                     /locus_tag="PLY56_10435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ21943.1"
                     /translation="MQIDPAKKYRATIVTNRGTIELELFPEFAPKTVNNFVFLSREGF
                     YDGLTFHRVISNFVIQGGDPTGTGSGGPGYRFEDEVRNNPLKHEKGVISMANAGPNTN
                     GSQFFITHSPQPHLNGKHTVFGKVVAGQEVVDAIRQGDRMEKVTITEE"
     gene            553..1944
                     /locus_tag="PLY56_10440"
     CDS             553..1944
                     /locus_tag="PLY56_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009613014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ21944.1"
                     /translation="MSVFDRKRLPPETFKLDVERMRRGWYSDKYFANIVTLLTQLARH
                     GYRYEGSARALARLGLDAVSVDVGNIEVEMQWFPRRKPFCIVAGIDKALAILKECAGY
                     FDEDGRFVNTFDQMEVDAVHDGFVAPYAGDPSQVTPVLRVRGRYRDFALLETPTLGAL
                     TRGSRIATNVYEVLEAARGKAVMFFPARFDAHEVQAADGYAYCIAVEVFNRRNGHQLR
                     AMVSTDAQGGWWEGAGGGTIAHAAIACFLGDTAEMMLAFASVMPPEVPRVALVDFNND
                     CVGDSLRVMAALFARWMQLTDEGRLEEAQRFKLAAVRPDTGANLRDVSVPPLGDRRLD
                     AGVNARLVFTLREAIDNAWQAWNLPRKWHSRAQKWCRDVRIIVTGGFKPEKIREFEEL
                     GVPVDIYGVGSYLLSSCDIHGTNNDYTADVVRVKVDGKWYDMAKAGRAPGVNPALEPV
                     SAAARERAERMAE"
     gene            complement(2055..3302)
                     /locus_tag="PLY56_10445"
     CDS             complement(2055..3302)
                     /locus_tag="PLY56_10445"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOJ21945.1"
                     /translation="MESLNPFEIAQAQLDEAAAVLDLDPATHQLLRVPMRELHVSIPV
                     RMDDGTTKIFTGFRVQYNDACGPTKGGIRFHPEETIDTVRALAAWMTWKTAVVGLPYG
                     GGKGGIICNPKQMSEGELERLARGYIRSLGNFIGEERDIPAPDVYTTPRIMAWMMDEY
                     SKMTGHNVPGLITGKPLILGGSQGRGDATARGAIYCIREAAKLLGLELKDGTLAIQGY
                     GNAGVYLAQLAVELLGMRVVAISDSCGGIYRADGIDWQMALEHKKATGSVAGLPGTTP
                     ITNEELLLLDVTVLCPAALENVITSRNAGEVKAKVIAEAANGPTTPAADRILHEKGIF
                     VIPDFLCNAGGVTVSYFEWVQNVTGYYWDLPTVHERLDNLMTEAFWRVARVRESRNVH
                     TRLAAYVVAVDRVAQAVRLRGWV"
BASE COUNT          666 a         1201 c         1216 g          687 t
ORIGIN      
        1 cgaccgtagc gcgccttccg caggcgtaga gaggaaacgc gcttggcacg caagcagtgg
       61 agcaccccgc cggcgatgca gatcgacccg gcgaagaagt atcgggccac cattgtgacg
      121 aaccggggca ccatcgagct ggagctcttt cccgagttcg ccccaaagac agtgaacaac
      181 tttgtcttcc tgtcacgcga gggcttctat gatggtctca ccttccatcg cgtcatcagc
      241 aacttcgtga tccagggagg cgaccctacc ggcaccggct ctggcggccc cggctatcgc
      301 ttcgaggatg aagtccgcaa caaccccctg aagcacgaga agggcgtcat ctccatggcg
      361 aacgccggcc cgaatacgaa tgggagccag ttcttcatca cccattctcc ccaaccccat
      421 ctcaatggca agcataccgt attcggcaag gtggtggccg gccaggaggt ggtggacgcc
      481 atccgccagg gtgatagaat ggagaaggtc acgattaccg aggagtgacc tatcgggagg
      541 ggagcgagca cgatgagtgt tttcgaccgg aagcggcttc caccggagac gttcaagctg
      601 gatgtggagc gcatgcgccg cggctggtat tccgacaagt acttcgcgaa catcgtcacg
      661 ctcctgacgc aactcgcgcg ccacggctat cggtatgagg gctctgcccg tgcccttgcc
      721 cgcctcggtt tggacgcggt cagcgtggac gtcggcaaca tcgaggtgga gatgcagtgg
      781 ttcccgcgtc gcaagccgtt ctgcatcgtt gccggtatcg ataaggctct ggcgatctta
      841 aaggagtgtg ccggctactt tgatgaagac ggccgcttcg tcaacacctt cgatcagatg
      901 gaggtcgacg ccgtccacga cggcttcgtc gcgccttatg ccggtgaccc gtcccaagtg
      961 acgcccgtcc ttcgggtccg cgggcgctat cgtgacttcg cgctgttgga gacgccgaca
     1021 ctgggtgcgc tgacgcgcgg gagccgcatc gccacgaatg tctacgaagt gctcgaggcg
     1081 gcgcgcggca aggcggtgat gttcttcccg gcgcgtttcg acgcgcacga ggtacaggcg
     1141 gccgatggct acgcctactg tatcgccgtg gaggtgttca accggcgcaa tggccatcaa
     1201 ctgcgggcga tggtctccac cgatgcccag ggaggatggt gggagggcgc cggtgggggc
     1261 acgattgccc atgcggcaat cgcgtgcttc ctgggagata cggcggagat gatgctcgcc
     1321 ttcgcctcgg tgatgccgcc ggaggtgccc cgcgtcgcgc tcgtcgattt caacaacgac
     1381 tgcgtgggcg actcgctccg cgtgatggca gctctgttcg cccggtggat gcagctcact
     1441 gatgagggtc ggctggagga agcgcagcgc ttcaagctgg cggccgttcg ccccgatacc
     1501 ggcgccaatc tgcgcgatgt gagcgtgccg cccctgggcg acaggcgcct cgatgctggc
     1561 gtcaacgccc ggctcgtctt cacgctccgc gaagcgattg ataacgcctg gcaggcctgg
     1621 aatctcccgc gaaagtggca ctcgcgggcg cagaagtggt gccgcgatgt gcgcatcatc
     1681 gtcaccggcg ggttcaagcc ggagaagatc cgcgagtttg aagagctggg cgtgccggtc
     1741 gatatctatg gcgtcggctc ctatctcctc tcctcctgcg acatccatgg caccaacaac
     1801 gactacaccg ccgatgtggt gcgggtgaag gtggatggaa aatggtacga catggcgaag
     1861 gcaggccggg ccccgggggt gaaccccgct ctggaaccgg tttcggccgc ggccagggag
     1921 cgcgcggagc ggatggccga gtgaggcccg gagtgcctcc cggataagcg cggaaggggg
     1981 ggaagaaccc gaagagagag cgctctcctc ggggccttcc cccctcctga taaaacgctc
     2041 ctctttcgag cgcgttacac ccagccgcgg aggcggaccg cctgcgctac gcggtccacg
     2101 gctaccacgt aggcggcgag ccttgtatgc acgttccggc tctcacgcac gcgtgccact
     2161 cgccagaacg cctcggtcat caggttgtcg aggcgctcgt gaacggtggg cagatcccag
     2221 tagtagccgg tcacgttttg cacccactcg aagtaggaga cggtgacccc gccggcgttg
     2281 cacaggaagt cgggaatgac gaagatccct ttctcgtgca ggatacggtc agctgccgga
     2341 gtggtagggc cgttcgctgc ctcggcgatc accttcgcct tcacctcgcc ggcgttgcgg
     2401 ctggtgatca cgttttcgag cgcggcgggg cacaggacgg tcacgtcaag caggagtagc
     2461 tcctcgttgg tgatcggggt ggtgccggga aggccggcca cggagccggt cgccttctta
     2521 tgctcgagcg ccatctgcca gtcgatgcca tccgcgcggt agatgccgcc gcagctgtcg
     2581 ctgatggcca cgacgcgcat ccccagcagc tcgacggcca gctgcgccag gtagacgccg
     2641 gcattgccgt agccctggat ggcaagcgtg ccatccttca gctccaggcc aagcagcttg
     2701 gctgcctcgc gaatacagta gatagcgccg cgcgccgtgg catcgccacg gccctgagag
     2761 cctcccagga tgagcggttt gcccgtgatg agaccaggca cgttgtgccc ggtcatcttg
     2821 ctgtattcat ccatcatcca cgccatgatc cgtggcgtgg tgtagacatc gggcgcggga
     2881 atgtcgcgct cctcgccaat gaagttgccc agggagcgga tgtagccgcg cgccagccgc
     2941 tcgagctcgc cttcgctcat ctgcttcggg ttgcagatga tcccgccttt gccgcctccg
     3001 tagggcagtc ccaccaccgc ggtcttccag gtcatccacg ccgccagggc gcgcacggtg
     3061 tcgatcgtct cctcggggtg gaagcggatc ccgcctttgg tcggtccaca cgcatcgtta
     3121 tactggacac gaaacccggt gaagatcttg gtcgtgccat catccatgcg gactggaatg
     3181 gagacgtgaa gctcgcgcat cgggacgcgg agaagctggt gcgttgcggg gtcgaggtcc
     3241 agcacagcgg ccgcctcgtc caactgcgct tgtgcgattt caaacgggtt tagtgattcc
     3301 attgctcgtt ccacgggtgt tctatctttc tcctgcagtt catacccaga gccgccaggg
     3361 ttgccagcat catctcgccc ctcagcctgc gcccgcttca tcccttctcg ccccgaggcg
     3421 gaaggcatca gggatcgggc ggagatgggt cgaatcccca cgcattccgc gaaccacccc
     3481 tttggccgac cccatcctcc tgggtgtctc cagaccagcg cgaccggccg ccatgggccc
     3541 agcacgcgcg cgagcccttc caacacgctg ggcagcagac ggcaaccggt tgccgacagc
     3601 cgacttatta tcccccaaat gggccgtagt gtcaagaggg aaaacgcatg gcgatccgac
     3661 aagccatcgc gcgtgtggcc cgattaggaa aatcgaagcc tctggccacc agccacccgc
     3721 ctatcaaccg cgtgcagtga gggcctgtgt tcaccagagg tcgtgagagc
//
LOCUS       DAOSCV010000400         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15448_length_3765_cov_3.216442, whole genome shotgun sequence.
ACCESSION   DAOSCV010000400 DAOSCV010000000
VERSION     DAOSCV010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15448_length_3765_cov_3.216442"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..904)
                     /locus_tag="PLY56_10450"
     CDS             complement(311..904)
                     /locus_tag="PLY56_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOJ21946.1"
                     /translation="MKGLSPKQKQVLEILEDAVREGQHLTVREIGDRIGVSSTCTVHQ
                     HLKALERKGYLKPTNHRHRSIQLTSPPRPFVPVPVGGNAVAGEAQGHDEPLPERMLVP
                     SEWVGQDESVLFRVVGNGLADAAIRDGDLVLVTRGREPSSGDVVVTQKGEQAELSWVT
                     AENGCLCVRNGGTTAIPLTQESELTVLGRVSVVIRRL"
     gene            complement(1554..2195)
                     /locus_tag="PLY56_10455"
     CDS             complement(1554..2195)
                     /locus_tag="PLY56_10455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ21947.1"
                     /translation="MKSAAIIPAYNEAPRITRILEVVTTLPQLAEIVVVDDGSADDTA
                     AVARAVDGVRVIRLETNQGKGGAMAAGVAATDASVLLFLDADLVGLKQQHVLDLLDPV
                     VAGEVDMSVGIFRGGRVFTDLAQVLVPYISGQRALVREVFTTLPGPEQRRSGIEAHLT
                     RHARASRYRVRHVAIEGVTHAMKEEKLGLARGAVARARMYSEILRVLLTPLGS"
     gene            complement(2208..2984)
                     /locus_tag="PLY56_10460"
     CDS             complement(2208..2984)
                     /locus_tag="PLY56_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOJ21948.1"
                     /translation="MMKVWMRPRRWLLIFTLLALFPAGCGGKLVGHAEEMRLGHEAAR
                     VIEQRFRVCQDQELQWLVERIGRRIAASSSRSDVDFTFRVLEEREINALSVPGYVYVN
                     RGLINATNANPDELAAVIAHEVAHTTERHMAKQMERVYGATFLLNAFVTENANINTLA
                     EIAVELALRGNSRQDERAADAQAVRFMSRAGYDPYGMVRFFERLLKETGDTRGLNKYL
                     STHPSTSERISRIRALIEKEDLARKALQPRRSSDVQIADH"
     gene            complement(3040..3447)
                     /locus_tag="PLY56_10465"
     CDS             complement(3040..3447)
                     /locus_tag="PLY56_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21949.1"
                     /translation="MRVIATGLILVALGLSLYSFLEVRRLRTEVVSLRAEVSTKKEED
                     SREARSRELLKSAEEHSKRAQELIRKGDIEGARREMRKGMELVTESAQISSGNDLAVQ
                     VREGAEGMLRRIEELLPRLKKTSSDPKTTQAKE"
BASE COUNT          662 a         1276 c         1122 g          705 t
ORIGIN      
        1 cgcacgcagg ccaaggacga gccctgcccc gatcaccaac agcgcgatcc ccgcgattag
       61 catggcgacc tcctctcgac cagatccgac gcgacgcggc tatccggtga tgccgcatcg
      121 ctgccgcgtc ccctaatacc gtgcgggcgt ctccttccca tcacctgtct gccagtactc
      181 tcctcactgc tccctcgctg ggaacgagga gcggcttccg caccgcaccg ggccgcctct
      241 tcacgccggg caggaagtga gcacgcggtg atcactgttg ggctcaacac gtcgaccccg
      301 cgtcggatgc tcagaggcgc cggatcacga ccgagacccg gcccagcacg gtcagctcgc
      361 tctcctgggt caacgggatt gccgtcgtgc cgccattgcg cacgcacagg cacccattct
      421 cggcggtcac ccagcttagc tccgcctgct cgcctttttg tgtcacgaca acgtcgccac
      481 tcgagggctc gcgcccgcga gtcacgagca cgaggtcgcc atcccggata gccgcgtcgg
      541 ccaggccatt accgacgacg cggaagagta cgctctcatc ctggcccacc cactccgagg
      601 ggactagcat cctctcaggc aggggctcgt cgtggccctg tgcctcgccg gccacggcat
      661 tgccgccgac tggaactgga acaaaggggc gaggggggga tgtgagctgt atggagcgat
      721 gacggtggtt ggttggcttg agatagccct tgcgctcgag ggctttcaga tgttgatgga
      781 cggtgcaggt tgaagagacc ccgatgcgat ctccgatctc tctgacggtc aaatgttgac
      841 cttcgcgtac tgcatcctcg aggatctcca acacctgctt ttgcttcgga gaaagccctt
      901 tcataaagat ccgggggtgc agggggagga aagcctcctg gctcctcacc ccctcattcc
      961 cccgcttctg gggggagcga ggggatcggt gggatggata gccccctgca gttcccgtcc
     1021 tcctctcacg tttcattctg tcgtatcata tctcgcgcag caccgtcctt cggcttttcc
     1081 gaacggaagc attctcgcga gggcacttca gggttacact gttgagggcg agccgtgaag
     1141 gctgccgacc cccgtcgcct cattgagcag gcgaccaggg tgttctccgc tttctgagat
     1201 ggggcgcccc agaaagggga acatcggtcc aagtgtcaga tggccatctc gatccgcgca
     1261 acaaaccgct gctacgaccc ggtggcgcta ctctcgggat ttggcccgga gtactcgccg
     1321 gcaggtctgc gtctctcctt tcatgcggct ggcgcggata cccgtagggc ctgctgccag
     1381 gcgtgctcat tgtatcatac gtatgttagg cacgtcaaac cgtgcccctc agacgtgcgc
     1441 tctccgatag gtggctgatg cccattacag gcagcgtccg ccccatcggc ggcacgggcc
     1501 gaaccggcgc gggggtaccg gggatcgctc gcggggccgc acggtgggcg ggctcacgag
     1561 ccgagcggtg tcagcagaac gcggaggatc tcagagtaca tgcgggcccg agcgacggct
     1621 ccacgcgcca ggcccagctt ctcctccttc atggcgtgcg tcacgccctc gatcgcaaca
     1681 tggcgcacgc ggtaacggct cgcccgggcg tgccgcgtca ggtgggcctc aatgcccgag
     1741 cgtctctgct ccgggcccgg gagcgtcgtg aacacctctc ggaccagcgc ccgctgcccg
     1801 gagatgtagg ggaccaatac ctgtgccaga tcggtgaaga cacgcccgcc ccgaaagatc
     1861 ccaacggaca tatccacctc gccggcgaca acggggtcga gcaggtcgag cacgtgttgt
     1921 tgcttcaggc caacgaggtc ggcatccagg aagagaagga cagaggcatc cgtcgcggca
     1981 acacccgcgg ccatggcccc gccctttccc tggtttgtct caagacggat gacccgcacc
     2041 ccgtctacgg cacgcgcgac cgcagcggta tcatccgcgg agccatcatc gacaaccacg
     2101 atctcggcga gctgtggcag agtggtaaca acttcaagga tccgcgtgat gcgcggcgcc
     2161 tcattatagg cggggataat agcggcactc ttcaacgctg gtattcctca atggtccgcg
     2221 atctgtacat cggacgagcg ccgaggctga agcgccttac gcgccagatc ttccttctcg
     2281 ataagggcgc gtatgcggga tatgcgctcg gaggtcgaag ggtgggtgga caagtactta
     2341 ttgagaccgc gggtatcgcc cgtctccttg aggaggcgct cgaaaaaccg gaccataccg
     2401 taaggatcgt atccagcccg gctcatgaaa cgcacggcct gagcgtcggc ggcccgctca
     2461 tcctgccggc tgtttccgcg aagcgccagc tctaccgcga tctctgcgag ggtgttgata
     2521 tttgcattct ctgtgacgaa tgcgttgagc aagaacgtcg ccccgtacac gcgctccatc
     2581 tgcttggcca tatggcgttc ggtggtgtgc gccacttcat gggcgatcac agcggcaagc
     2641 tcgtcggggt ttgcgttcgt ggcgttgatc aggccccggt tcacatagac gtaaccggga
     2701 acagagaggg cgttgatctc gcgctcttcc agcacacgga aggtaaagtc gacatcgctg
     2761 cgcgagctgc tggcggcgat gcggcgccca atccgctcga ccagccactg cagctcttga
     2821 tcctggcaga cgcggaagcg ctgctcgatg acgcgtgccg cctcatgtcc gaggcgcatc
     2881 tcctccgcgt gcccaaccag ctttcctccg caacccgccg ggaagagcgc gagcagcgta
     2941 aagatcagaa gccatcgccg cgggcgcatc caaaccttca tcataccctc cgcgggaacg
     3001 gccaggccac gggcgaagcc gggcaaccgg cacagccggt cactccttcg cctgcgtggt
     3061 tttcggatcc gaacttgtct tcttcaagcg tgggagtaac tcctcgatgc gccgcagcat
     3121 gccttctgcg ccctcacgaa cctgcaccgc cagatcgttg ccactgctga tctgggccga
     3181 ttcggtcacg agctccatgc ctttgcgcat ctctctgcgg gcgccctcga tatcgccctt
     3241 tcgaatcagt tcctgcgctc tcttcgaatg ctcctcggca ctcttgagca actcgcgact
     3301 gcgggcttcg cgcgagtcct cctccttctt cgtcgagact tcggcgcgca ggctcaccac
     3361 ctcggtgcgg aggcggcgca cttcgaggaa gctatagagc gagagcccca aagccaccaa
     3421 tatgagccct gttgcaatca cacgcatacc tgcatccttc gaggctacac gccatcctct
     3481 ggcagcctca cccggagccg cgcgccctct tcgcgcccgc ccggcacctt gccttcgaga
     3541 cgcccggacg ccacgcgtgc cagcgttctg tgcaagcaag cgccagctcg ctgacgctcc
     3601 tctcgctctc tatggccgcg attataccag aacgggcgcg tttgtgtaaa gctggcctca
     3661 gaagtccgcg ctaatgctgg cgacactctc tgtcgcgcgc tcaccctcgc ggcctacaag
     3721 aaccgcgcca tttagcgtat agccctggcc acgatagccc agccc
//
LOCUS       DAOSCV010000401         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15456_length_3763_cov_2.440129, whole genome shotgun sequence.
ACCESSION   DAOSCV010000401 DAOSCV010000000
VERSION     DAOSCV010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15456_length_3763_cov_2.440129"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLY56_10470"
     CDS             <1..838
                     /locus_tag="PLY56_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ21950.1"
                     /translation="MANKYLGATVDIHGGGIENIFPHNEDEIAQSEAANDAPYVRYWL
                     LTGSLKIGGQKMSKSLGNFVTIEQALENHSAEALRFYLLQAHYASPIDFSWDPERKVS
                     PSIEEAQRGLDRLYGALRAADEWLQGAPPAAEGEASADAQALEARRAESHAEFYEAMD
                     DECNTPRAIAVLFTLAAAINRFATGAVPAAGTDRAIVQAARDELCVLAGVLGLLEQGA
                     VQESAATDLDAVIEKIIAWRQDARQRKEFALSDRIRDDLRDVGILLEDHPGGKTTWKR
                     AR"
     gene            1117..2115
                     /locus_tag="PLY56_10475"
     CDS             1117..2115
                     /locus_tag="PLY56_10475"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ21951.1"
                     /translation="MLEKHLLPALERKIPLVDGPRQWEVTPRELGCSYDLDATIEKAL
                     SIGRQGNLLRRARDGWEARRRGVVIKPRVEFDRKAMRARLKRLARATDIPARNATIRF
                     DGREFVIGAERVGRVLDVDATEKNIVRYFVPHSHRDLRLVVREDRPRITAAELSQIDG
                     RISTYTTRYNPADVNRAVNLRLAARALDGTIVPAGGVFSYNTVVGPRTEARGYRVAKI
                     FQKGEILDGIGGGICQVSTTLYNAALEGRMRILERAQHSRPVTYAPPGRDATVSYGGV
                     DFRFRNTTGAPILIQTYARAGVLTVDIYGKRPVRQTGKRPGAAARGTTRDLVHGTG"
     gene            2235..2867
                     /gene="sigH"
                     /locus_tag="PLY56_10480"
     CDS             2235..2867
                     /gene="sigH"
                     /locus_tag="PLY56_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HOJ21952.1"
                     /translation="MGSCADDAPITFSGMLDEEVVLYAKNGSDRAVDYLLQKYRSLVE
                     SKARLYFLVGADHDDVVQEGMIGLYKAIRDFRDDKSKFRAFAELCVTRQIITAIKTAT
                     RQKHVPLNSYVSLHKCIADEESDQTLIDVIPDSRMVNPEEIVLGRQLAHYIDHGMDHD
                     LSRLEAQVLRQYLEGKTYKEMADDLKCRTKSIDNALQRAKRKISERMERD"
     gene            2872..2946
                     /locus_tag="PLY56_10485"
     tRNA            2872..2946
                     /locus_tag="PLY56_10485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2904..2906,aa:Thr,seq:tgt)
     gene            2954..3039
                     /locus_tag="PLY56_10490"
     tRNA            2954..3039
                     /locus_tag="PLY56_10490"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2988..2990,aa:Tyr,seq:gta)
     gene            complement(3105..>3763)
                     /locus_tag="PLY56_10495"
     CDS             complement(3105..>3763)
                     /locus_tag="PLY56_10495"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HOJ21953.1"
                     /translation="MAMAASGRLTPVAAAILHQAGSLLVLLNSMRLLGFGGWRTSPVA
                     RWVHDAEHALESWDSRFDLGPVLERTPHLARKAAVALGLLLIRVTGWSFLDPLVALVL
                     AIVIGRIAWELSHRAAHPLLDTRLPAEEERELVRWIEEHPAVMGCHCVRSRSAGAWAY
                     LDAHVTVAAGTTLEDADALEEELRAQLVKRRPRLDVMLHIEPHPETLPRGREGQAERA
                     "
BASE COUNT          695 a         1230 c         1196 g          642 t
ORIGIN      
        1 catggcgaac aagtacctgg gcgccaccgt agacatccac gggggcggga tcgagaacat
       61 cttcccgcac aacgaggatg agattgccca gtcggaagcg gcaaacgacg cgccctatgt
      121 gcgctattgg ctcctcaccg gcagcctgaa aatcggcggc cagaagatga gcaagtcgct
      181 gggcaacttc gtgacgatcg agcaagccct ggagaaccac tccgctgagg cgctccgttt
      241 ctacctgttg caggcccact acgccagccc cattgatttc tcgtgggatc ctgagcggaa
      301 agtctcgccc agcattgagg aggcgcagcg cggcctggat cgcctctatg gcgcactgcg
      361 cgccgctgac gaatggctgc aaggggcccc tcctgccgcc gagggcgagg cgtctgctga
      421 cgcccaggcg ctggaggcac gccgcgcgga gagccacgcc gagttctacg aggcgatgga
      481 tgacgagtgc aacacgccgc gtgccattgc cgttctcttc acgctggcag ctgccatcaa
      541 ccgctttgcc accggcgccg tgcccgccgc cggcaccgac cgggcgattg tccaggctgc
      601 ccgcgatgag ctgtgcgtcc tggcaggcgt gcttggcctc ctggaacagg gcgccgtgca
      661 ggaatccgca gcgaccgatc tcgacgcggt gattgagaag atcatcgcat ggcgccagga
      721 tgcgagacag cgcaaagagt tcgctctttc tgatcggatc cgagacgacc tgcgcgatgt
      781 ggggatcctc cttgaggacc acccgggcgg caagacaacc tggaaacgag ctcgataaga
      841 ccgccgatgg aaagacgccc tcgatccacc acagccccgc atgacgacac ctccggtggc
      901 tcctgggctg ccggcccggc atcctggtcc ttgccgcggc ggcgcggagc gcgtgcccgt
      961 cgatggaccg gtctgctgct tgggagcgcg cttctctctg gatcggcgct cctgggggcg
     1021 accttgggtc tgcgcccccc gccaggtcgg attctccccg gcgtcgctgt ttggcagatc
     1081 gacgtgggcg cgcgcacgcc acaagaagcc cgcgtcgtgc tagagaagca cctgcttccc
     1141 gccctggaac gcaagattcc cctcgtcgat ggccctcgcc agtgggaggt cacgccccgc
     1201 gaactggggt gcagctatga cctcgacgcg actatcgaga aggctctgag catcgggcgc
     1261 caggggaacc tgttgcgacg cgctcgcgat ggctgggagg cacgccggcg gggtgtcgtc
     1321 atcaagccgc gggtggagtt cgaccgcaag gcgatgcgcg cgcgcctgaa gcggttggcg
     1381 cgcgccacgg acattcccgc ccgtaacgcc accatccgct ttgatgggcg cgagttcgtg
     1441 atcggcgcag agcgcgtggg gcgcgtgttg gatgtcgatg ccaccgaaaa gaacatcgtg
     1501 cgctacttcg tgccgcattc ccaccgcgac ctgcgtctcg tcgtccgcga ggaccgcccg
     1561 cggatcaccg cggcggagtt gagccagatt gatgggcgca tctccaccta caccacgcgc
     1621 tacaacccgg ctgacgtcaa ccgcgccgtg aatctgcggc tggcggcgcg cgcgctcgat
     1681 ggcaccattg ttcctgccgg tggcgtcttc tcctacaata ccgtcgtcgg gccgcgcacc
     1741 gaggcgaggg gctaccgcgt tgcgaagatc tttcagaagg gggagatcct cgacggcatc
     1801 ggtggcggca tctgccaggt gagcaccacc ctctataatg ccgccctgga aggccggatg
     1861 cggatcctgg agcgcgccca gcattcgcgc ccggtcacct acgccccccc cggccgcgac
     1921 gcgaccgtgt cttatggcgg cgtcgatttc cgctttcgga acacgaccgg cgcgccgatc
     1981 ctgatccaga cgtacgcgag agctggcgtg ctcaccgttg acatctacgg gaagcgcccg
     2041 gtgcgccaga caggaaaacg ccctggtgct gcggcccgcg gcactactcg cgatctggtg
     2101 catggcacag gctaattttc atgcccgcag cctattgacg aaatgagcag tcgatagtat
     2161 aataggcgtg gatggaacgc ggaggcattc ccaatcgacc agcgggctcc gcgtgtagga
     2221 cggaggcagc gcccatgggt tcgtgcgccg acgacgcccc gattaccttc tccgggatgc
     2281 tggacgaaga ggttgtcctc tatgccaaga acggaagcga tcgggcagta gattacctgt
     2341 tgcagaagta caggagtctc gtcgaaagca aggcgcggtt gtactttctc gttggcgcgg
     2401 accacgacga tgtggttcaa gaagggatga tcggcctcta caaggcgatc cgggattttc
     2461 gtgacgataa gtccaagttc cgggcgtttg cagagttatg tgtgacgcgc cagatcatca
     2521 ccgccatcaa gacggcaacc cgtcagaagc acgtgccgct gaactcttac gtctcgttgc
     2581 ataagtgcat cgcggacgaa gagtccgatc agacgctgat cgacgtgatc ccggatagcc
     2641 ggatggtcaa ccctgaggag atcgtcctcg gtcgccagtt ggcgcactac attgaccacg
     2701 gcatggatca cgacctcagt cgcctggagg ctcaggtttt gcgccagtac ctggagggta
     2761 agacctataa ggaaatggca gatgacctga aatgccgaac caagtccatc gataacgcgc
     2821 ttcagcgtgc gaagcgcaaa atcagtgagc gtatggagcg tgactgagac tgccggcgta
     2881 gctcagtggc agagcagctg ttttgtaaac agcttgtcat ccgttcgaat cggatcgtcg
     2941 gctccagttt cgtgggtggg tgtccgagtg gctaaaggag acggtctgta aaatcgttgg
     3001 cgtacgccta cagaggttcg aatcctctcc cacccaccag caaggggcga agcaccgcaa
     3061 gggcttcgcc ccttctgatt catcgccacc gccgctccgg gctgctacgc ccgctctgcc
     3121 tggccttccc gaccccgtgg caacgtctcc gggtgcggtt cgatatgcag catcacatcc
     3181 agccgcggcc ggcgcttcac gagttgcgcg cgcaactcct cctccagtgc atccgcatcc
     3241 tccagcgtgg tgcctgctgc aacggtgacg tgcgcatcga gataggccca ggcccctgcc
     3301 gagcgcgagc ggacgcagtg gcatcccatc acggccgggt gttcctcaat ccaccgcacc
     3361 agctccctct cctcctctgc cggcagacgc gtatccagaa gcgggtgcgc cgcgcgatgg
     3421 ctcagctccc aggcaatccg accgatcaca atcgccagca cgagcgccac gagcggatcc
     3481 aggaagctcc acccggtcac ccgaatcaac agcaggccga gcgcgacggc cgccttgcgg
     3541 gcgagatgcg gcgtccgctc gaggacgggg cccaggtcga atcggctgtc ccaggattcc
     3601 agcgcgtgtt ccgcgtcgtg gacccaacgg gcgacgggtg aggtcctcca gccgccgaac
     3661 ccgagcagtc gcatcgaatt caagaggacg agcagggaac ccgcctggtg caggatcgcc
     3721 gccgcgaccg gcgtcagcct ccccgacgcc gccatcgcca tcg
//
LOCUS       DAOSCV010000402         3759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15480_length_3759_cov_3.458153, whole genome shotgun sequence.
ACCESSION   DAOSCV010000402 DAOSCV010000000
VERSION     DAOSCV010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15480_length_3759_cov_3.458153"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3759
                     /locus_tag="PLY56_10500"
     CDS             <1..>3759
                     /locus_tag="PLY56_10500"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ21954.1"
                     /translation="AAEGPAKFGETGLGVDAAWRLIKGNSAEELRADLLKKLEKEYDV
                     IVVASFQWDLLPIEARYEILKKVKAGTGLAGLITGRDEFLGKLLPETSFAWNWSVWSG
                     AAEGISDYFGKGVFTGRIDYETRRSGDGSVRITCTEAVKGSRESPRAGYSQTNIPVEK
                     NTRYRFSVWYRTRGGRGTSISFHPLGGLTLEPSDEWKQATAVIDTKDHDRFNVYLLAY
                     HVGDVWYDDARLVKEGGDGTNLLKDSELEKPRSAVDEPFLTRGVPYAALPAFARIASA
                     REFGESVLQTGQFRRGRLCVLRGFTPPRNQGMTPGLSGSALTASSLDYDYYLSLAIRA
                     ILWAAGKVPAVQIETPEAPRTVLQRESGGRVRFTLSASVPQADLSARFALRARDGHVW
                     DTREEKLAVSGTASLDYVVPRVPAGDYFVDLWVKHAGKTVNWASAAVTVEAADRLSEA
                     KIPESIATGEPVTGKVTVTLKTPGAKQLRLRQYDNYGRLVGERVLPVPTGGGEVAFSL
                     PASRPLSLLQHLEIALLSAGEPVESVRLPFFYRDFFPPRDDIRWVMWDGMAGDSFIGR
                     IIAREFRKAGLDTQYTGLSEWAIRENMWHIPYVTRFVDRKTDWYQEKRTRTQDDLVRD
                     PCLTDPAYRAKLREDLLQAVQRVTRFGTGEFSLGDENHFVAGNFDLCMSPTCVADFRE
                     WAKREYRGDLNALNKEYGTAYTSWEQVRPATLEEARKSGNYAPWVDHRRHMETVWAGI
                     HQYAREVIREAVPGARVGYEGSDTRAGTYHAADYWQIMRAMDLNNLYYRDFQGAAVRD
                     FADPGTLYGAGWYGGYADNRNEEFMRWYPWMVLFQGTNSYWVWMGCGSAGAVMAFDAS
                     LYPFFAANAEEMREIKGGVGKLLMHAERQHDGVAVYWSPVSVHVREFTPGMPEADDAL
                     NSTVRVLDDVQVQPRVLSYAELEKLTPQRFKLLFLLQAQALSDAEAEAIRRFVRDGGI
                     VIADLRPGVRNEHGTARATGALDDLFGVKQNADAPDLKKAEPELLQPARKLPQAVVDA
                     SLRVAGGNATGRAGETPVMIQNTFGKGKAILLNFSWADYLARERTARTAGADYAFWPI
                     NEEWRALVRVLCENAGVVPPVSMAPSLPRTQVVRFRSGNAEYVGVLAGLPRDPNAYTA
                     KEAKLPAPRQTTIRFGRTAHAYDVRAGRYLGKVDQVKTLVQAGRARLFALLPYAVSEI
                     RVSTSAVSLGQETRVRAECITGGKPGRHVLRVRFIGPDGKERREYQQSV"
BASE COUNT          619 a         1190 c         1319 g          631 t
ORIGIN      
        1 gccgccgagg ggccggcgaa gttcggggag acgggcctcg gcgtcgatgc cgcgtggcgg
       61 ctcatcaagg gcaactccgc ggaggagcta cgggccgacc ttctgaagaa gctggagaag
      121 gagtacgacg tgatcgtcgt ggccagcttc cagtgggatc ttctccctat cgaggcgcgc
      181 tatgagatcc tgaagaaggt gaaggcgggc accggcctgg ccggccttat caccgggcgc
      241 gacgagtttc tcgggaagct gttgccggaa acctcgtttg cctggaactg gtccgtctgg
      301 agcggcgccg cggaaggaat ctcggactac tttgggaagg gcgtcttcac cggccgcatt
      361 gattacgaga cgcggcggtc cggggatggg agcgtgcgga tcacctgcac cgaggcggtg
      421 aagggcagcc gcgagagccc ccgagcgggc tactcgcaga ccaacatccc ggtggagaaa
      481 aatacgcgct atcgcttctc ggtgtggtac cgaactcgcg gcggacgcgg aacgagcatc
      541 tcgttccacc ctctgggcgg ccttactttg gagccctccg acgagtggaa gcaagccacg
      601 gctgtgatcg atacgaagga tcacgaccgg ttcaatgtct acctgctggc ctaccacgtt
      661 ggcgatgtct ggtacgatga cgcgcgcctc gtgaaggagg gcggggatgg caccaacctt
      721 ctgaaggact ccgagcttga aaagccgcgc agcgccgtgg atgagccttt ccttacgcgc
      781 ggagtgccct acgccgccct gccggcgttt gctcgcatcg ccagcgcccg tgagtttggg
      841 gagagcgtgc tgcagacggg acagttccgc cgcggccggc tgtgcgttct tcggggtttc
      901 accccgccgc gcaatcaggg aatgaccccg gggctttctg gcagcgccct caccgcgtct
      961 tcgcttgact atgattatta cctctcgctg gctatccggg cgatcctgtg ggctgccggc
     1021 aaggtgcccg ccgtgcagat cgaaactccc gaggctcccc ggaccgtcct ccagcgcgag
     1081 agtggcgggc gcgtccggtt cacgctttcg gcatccgtgc cccaggcgga tctgagcgcg
     1141 cgctttgcct tgcgtgctcg cgatggccat gtctgggata cccgcgagga gaagctggcc
     1201 gtctccggca cggcgtcgct cgactacgtg gtgccccggg tgcccgcggg cgactatttc
     1261 gtggacctct gggtgaagca cgccgggaag acggtgaact gggcctcggc ggcggtcacc
     1321 gtggaggcgg ccgaccgcct ctccgaggcg aagatcccgg agagcatcgc cactggagag
     1381 ccggtgacgg ggaaggtgac ggtgacgctg aagacgccgg gggcgaagca gctgcgcctg
     1441 cgccagtatg acaactacgg ccggctggta ggcgagaggg tgcttcccgt gccgacaggg
     1501 ggcggcgagg tcgccttctc cctgcccgcg tcgcgcccgc tgtcgctcct ccagcacctg
     1561 gagatcgcgc tcctctctgc cggggagccg gtcgagagcg tgcgccttcc cttcttctac
     1621 cgcgacttct tccccccacg cgacgacatc cgctgggtga tgtgggatgg catggcgggc
     1681 gattccttca tcgggcggat catcgcgcgc gagtttcgca aggcaggcct cgatacccag
     1741 tacaccgggc tctccgagtg ggccatccgc gagaatatgt ggcatatccc ctacgtgacg
     1801 cgcttcgtgg accggaagac cgactggtac caggagaagc gcacgcgcac ccaggatgac
     1861 ctggttcgcg acccgtgcct gaccgacccg gcgtatcgcg cgaagctgcg cgaagatctg
     1921 ctgcaagccg tgcagcgcgt tacgcgtttc ggcaccggcg agttctccct gggcgatgag
     1981 aaccatttcg tggccgggaa cttcgacctg tgcatgtcgc ccacgtgcgt agcggatttc
     2041 cgcgaatggg cgaagcgcga gtatcggggc gacctgaacg cgctcaacaa ggagtatggc
     2101 accgcctaca cctcgtggga gcaggtgcgt ccggcgacgc tcgaggaagc gcgcaagagc
     2161 gggaactatg ccccctgggt ggatcaccgc cggcacatgg agacggtgtg ggctgggatc
     2221 caccaatacg cgcgtgaggt gatccgagag gccgtgcccg gggcgcgcgt cggctatgag
     2281 ggctcggata cccgggccgg cacctaccat gccgccgact actggcagat catgcgcgcg
     2341 atggacctga acaacctcta ctatcgcgac ttccagggag cggcggtgcg cgacttcgcc
     2401 gatccgggaa cgctgtatgg cgccggatgg tatggaggct acgcagataa ccgcaacgag
     2461 gagttcatgc gctggtaccc ctggatggtc ctcttccagg gcaccaactc ctactgggtg
     2521 tggatgggct gtggctccgc gggcgccgtc atggcgttcg acgcatcgct ctacccgttc
     2581 ttcgccgcca acgcggagga gatgcgcgag atcaaggggg gtgtcggcaa gctgttgatg
     2641 catgccgagc gccagcacga tggcgtggcg gtctactggt cgcccgtgag cgtacacgtg
     2701 cgcgagttca cccccgggat gccagaggcg gacgatgcgc tcaactcgac cgtgcgcgtg
     2761 ctcgatgatg tgcaggtgca gccgcgcgtc ctctcctatg cggagctgga gaagctgacg
     2821 ccccagcgct tcaagctgct cttcctgctg caggcgcagg cgctctccga tgccgaggcg
     2881 gaggcaattc ggcgcttcgt ccgcgatggc ggcatcgtca tcgccgacct gcgccccggc
     2941 gtgcgcaacg agcatgggac ggcgcgagcg acgggcgccc tggatgacct cttcggcgtg
     3001 aagcagaacg cggatgcccc cgacctgaag aaagcagagc ccgagctgct ccagccggca
     3061 cggaagctgc cgcaggcggt ggtggatgcc agcctgcgcg tggcgggcgg gaatgccact
     3121 ggccgggcgg gcgagactcc ggtgatgatc cagaacacct tcggcaaggg gaaggcgatc
     3181 ctcctcaact tctcgtgggc cgactatctg gcgcgagagc ggacggcaag aacagccggc
     3241 gccgactacg ctttctggcc aatcaacgag gagtggcggg ctctggttcg cgtcctctgc
     3301 gagaatgcgg gtgtggtgcc gccggtctcg atggcgccgt cgctcccgcg cacgcaggtg
     3361 gtgcgcttcc ggagcgggaa cgccgagtat gtgggcgtgc ttgcagggct cccgcgtgac
     3421 cccaacgcct acaccgccaa agaggcgaag ctccccgcgc cccggcagac gaccattcgc
     3481 ttcggccgta cggcacacgc ctacgacgtt cgcgcaggcc gctacctggg gaaggtggat
     3541 caggtgaaga cgctggtgca ggcagggcgc gcccggctct ttgcgcttct gccttacgcc
     3601 gtctccgaga tccgggtcag cacgtctgcc gtctcgctgg gtcaggaaac gcgcgtgcgc
     3661 gcggaatgca tcaccggtgg gaagcccggc cggcacgtcc tgcgggtgcg gttcatcggc
     3721 ccggacggga aggagcggcg cgagtatcag cagagcgtc
//
LOCUS       DAOSCV010000403         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15552_length_3749_cov_3.134272, whole genome shotgun sequence.
ACCESSION   DAOSCV010000403 DAOSCV010000000
VERSION     DAOSCV010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15552_length_3749_cov_3.134272"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="PLY56_10505"
     CDS             complement(<1..1367)
                     /locus_tag="PLY56_10505"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOJ21955.1"
                     /translation="MTTPVTWDLSDLYVGVEDPKLTADLDRLDARAARFAEAYRGKVG
                     QLSPPDFAAALVEYEAIQRDVQNPSAFAQLVFAADSLNPAHGALVQLVQEREVAIGRQ
                     LLFFSLEIVELPDDHFARLLEDPTVAEYRHYLEHLRSERPHRLTEPEERILLEKSITG
                     RSAFQRLFDETIGAATFKMELHGATQEMNLSEILDKQHDADRAVRAASAAALTAGLEE
                     RSRIFSYITNTLAHDKAVDDRLTRFSHPEASRHLSDEVDTDTVHTMLDACVDHFGTVA
                     RYYHLKREMLGLDRLYHYDRYAPLFPEESVTPWEEARDIVVDAYTRFSPTVGDLARRF
                     FDQRWIDAEVRAGKRGGAFCMGIAPDWHPYVLMNYLGKQRDVLTLAHELGHGIHDLLA
                     TGQHFLEYHPSLAAAETASVFGEMLTFDALLARDLPAHDRLSLLTEQIEGIFATVFRQ
                     TAMYR"
     gene            complement(1605..2069)
                     /locus_tag="PLY56_10510"
     CDS             complement(1605..2069)
                     /locus_tag="PLY56_10510"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOJ21956.1"
                     /translation="MQGKRVCESLVTLTQLMHPEHANNLGNVHGGVVMRLIDEAGALC
                     AMRHARRPAVTVAVDSLTFLEPVHIGDLVTLNARLTYVGRTSMEVEVCVEAEEIRTDR
                     RVLTNRAYVVYVALDDEGKPVPVPPLLLETPEEKARAEEARARQARRLAARA"
     gene            2508..3005
                     /gene="greA"
                     /locus_tag="PLY56_10515"
     CDS             2508..3005
                     /gene="greA"
                     /locus_tag="PLY56_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ21957.1"
                     /translation="MAEPVIMTAAGKAKLEAELDELVNVRRPAVLLRIREAKALGDLR
                     ENFDFQDAKREQAFLEGRIQALKETLGRARVVSEEEMNSEGVGIGSTVEMVHLETGSV
                     CTYTIVGAVEADPAKGKISNASPVGKALMGQTEGATVTITTPRGVQQYRIQAIRRNHE
                     TTAVG"
     gene            3388..>3749
                     /locus_tag="PLY56_10520"
     CDS             3388..>3749
                     /locus_tag="PLY56_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HOJ21958.1"
                     /translation="MRAIPVKGRHWPRFPMVFVEVGTDEGVVGIGEALAFQTTGVVQS
                     VETVGEWLLGENPLQIERLWERCFRRGAELSALSGIEIALWDIAGQVAGMPIYQLLGG
                     ACHDRIRVYADGFFRGATP"
BASE COUNT          671 a         1160 c         1297 g          621 t
ORIGIN      
        1 aagcggtaca tcgcggtctg gcggaagacg gtggcgaaga tcccttcgat ctgctccgtc
       61 aggagggaga ggcggtcgtg cgccggaaga tcgcgcgcaa gcagggcgtc gaaggtcagc
      121 atttcgccga agacggaagc ggtttccgca gcagcgagcg agggatggta ctcgaggaag
      181 tgctggccgg tagcgaggag gtcgtggatg ccgtggccca actcatgcgc cagggtaagc
      241 acgtcgcgct gcttccccag gtagttcatc aacacatacg ggtgccagtc aggggcgatc
      301 cccatgcaga acgcgccgcc acgcttgccc gcgcgcacct ccgcatcgat ccagcgctgg
      361 tcgaaaaacc gccgggccag atcgccaacc gttggggaaa agcgggtgta ggcatcgacg
      421 acgatatcgc gcgcctcctc ccagggcgta acactctcct ccgggaaaag gggagcatag
      481 cggtcgtagt gatagagccg gtccagaccg agcatctccc gcttcaggtg gtagtagcga
      541 gcgaccgtgc cgaagtgatc cacgcacgca tccagcatcg tatgcacggt gtcggtatcc
      601 acttcgtcgc tcaggtgccg cgaggcttcc gggtggctga agcgggtcag gcggtcatct
      661 actgccttat catgcgccag ggtgttcgtg atgtagctga agatccggga acgctcctcc
      721 agcccggctg tgagcgccgc cgcggaagca gcgcgcaccg cgcggtcggc atcatgctgc
      781 ttgtcgagga tctcggagag gttcatctcc tgggtcgcac catggagctc catcttgaac
      841 gtggcggcac caatcgtctc gtcgaagagg cgttggaacg ccgaacgccc ggtgatcgac
      901 ttctccagga ggatccgctc ctccggctcg gtcaagcggt ggggccgctc gctgcgcaga
      961 tgttccagat agtggcggta ctcggcaacg gtggggtctt ccaacagccg ggcgaagtga
     1021 tcgtccggaa gctctacgat ttcgagggag aagaagagga gctggcgccc aatggcgact
     1081 tcacgctctt gaaccagctg aacgagcgcg ccatgcgcgg ggttgaggct gtcggcggcg
     1141 aaaacgagct gggcgaacgc gctggggttc tgaacatccc gctgaatcgc ctcgtactcc
     1201 acaagcgccg cggcgaagtc aggcgggcta agctgcccga ctttgccccg gtacgcctcg
     1261 gcgaagcgcg cggcgcgcgc atccagacga tccaggtcgg cggtgagctt tgggtcctct
     1321 acccctacat agagatccga taggtcccag gtcaccggtg tggtcattgg cccatccttt
     1381 ccgcagcccc agacgcgggc tcaggcgcga gagtggcccg caggaatcgt gccgctcttg
     1441 cgcgatagtg acggcgcgcc cctccaggga aggcgctcgc tctcctcacc agtgtacacg
     1501 gtggaatcgc gatcgtcaag ggccacgata cggcgcgcca tcggcggcag cgataccacg
     1561 gatgaagggc gccggccacg gatgggggag cgcggcgccg atacttacgc ccgcgcggca
     1621 aggcggcgag cctggcgggc gcgtgcctcc tcggcgcgag ctttctcctc cggcgtttcg
     1681 aggaggaggg ggggaacagg aacgggtttc ccctcgtcat cgagggccac gtagaccaca
     1741 taggcgcggt tcgtgagcac ccggcggtcg gtgcggatct cctcggcctc cacgcagacc
     1801 tccacctcca tcgaggtgcg cccgacatag gtgaggcgcg cgttcagcgt caccagatcg
     1861 ccgatatgaa ccggctcgag gaaagtgagc gagtccacag cgacggtgac ggcgggacgg
     1921 cgcgcatggc gcatggcaca cagggcgccg gcctcgtcga tgaggcgcat caccacgcct
     1981 ccatgcacgt tgccgaggtt gttggcgtgc tctgggtgca tcagctgggt gagcgttacc
     2041 aacgattcgc aaacacgctt gccttgcacg tgggtctccc ttcgtctgct ctttctacgc
     2101 cggccggcgc cggcctgtca acgacctcat cgcgcgggac gatgcgcgag caggcggcga
     2161 cgcctcatgc gagtaggcgg ttgcccgcgc cgtagggctt gcctgatggt tctgatggcg
     2221 gtataatagg aaagaccaca ggccggcgga agccggcacg aggtgcccag gagaccactc
     2281 tttcacggat ggcaacaccg agatgcctgc cgtggcgcgc aatttaccca ccccatcgtg
     2341 gcaggccgtc gtacgcgcgt cgggaagcaa catggagcgg tgtttagacc gggcgcggag
     2401 tggcgatggc cggcctgggc cggcccccca gggttgatcg gatcgcgccg atactccgcg
     2461 catgcgcggt atccactgtt cgcataaggg atgggaggac ggagtctatg gcagaacccg
     2521 tgatcatgac tgccgccggg aaggcgaagc tcgaggccga actggatgag ctggtgaatg
     2581 tgcgtcgtcc agcggtgctc ctgcgtatcc gcgaggccaa ggcgctcgga gatcttcgcg
     2641 agaacttcga ttttcaggat gccaagcgcg agcaggcttt ccttgagggc cgaattcagg
     2701 ccttgaagga gacgcttggg cgcgcgcgcg tcgtcagcga ggaggagatg aactccgagg
     2761 gcgtcggaat cggctccacc gtcgagatgg ttcacctgga aacgggaagc gtgtgcacct
     2821 acaccatcgt tggggcggtg gaggcagatc ccgcgaaggg gaagatctcg aacgcttctc
     2881 cggttggcaa ggcgctcatg ggccagaccg agggcgcgac ggtgacaatc accactccgc
     2941 gtggtgttca gcaataccgc atccaggcga ttcgccggaa ccacgagacc actgccgtcg
     3001 gttgataatc cgggcgggcg agctggtctc gccgcgccgg cctaaacagc gcgggcgaaa
     3061 acacccgcgc tgcttgtgtc gctcgaacgt aactccccgc gctgcttgcg ggcgaagccc
     3121 tacgggctgg ggccggatag acccgaaggg cctttgccgg gaagcagcgc gggcctctac
     3181 acctgcgccc gatctgaccc gcgcatggtg ggccctccat gcactgccct cggcccattt
     3241 cagcgagcag ccggtttccc acggggttga cctccacacg cccgccgggt ataatcaaat
     3301 atataatata ccgcccattg gcccgctggg gcaggaggga gaagggtcgg gcaggcaccg
     3361 gcccggacgc atatgaagat cacccacgtg agggcgatcc cggtcaaggg acgccattgg
     3421 ccgcgcttcc cgatggtctt cgtggaagtc ggcaccgacg aaggcgtggt cggaatcggc
     3481 gaggcgcttg cgtttcagac caccggcgtc gtgcaatcgg tcgagacggt gggcgagtgg
     3541 ctgctcgggg agaacccgct gcagatcgag cgcctctggg agcggtgctt ccgccggggg
     3601 gcggagcttt cggcgctgag cggcatcgag atcgccctgt gggacatcgc tggccaggtg
     3661 gccggcatgc cgatctacca gctcctgggc ggggcgtgcc acgaccggat ccgggtgtac
     3721 gccgacggct tcttccgcgg cgccactcc
//
LOCUS       DAOSCV010000404         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15596_length_3740_cov_3.357123, whole genome shotgun sequence.
ACCESSION   DAOSCV010000404 DAOSCV010000000
VERSION     DAOSCV010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15596_length_3740_cov_3.357123"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..>300)
                     /locus_tag="PLY56_10525"
     CDS             complement(94..>300)
                     /locus_tag="PLY56_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOJ21959.1"
                     /translation="EHDPYVRERVCTGLSFLGLRFDPERNAVRSGEREISAPGSGVRA
                     LVIPTNEELVIARETAEVVGARQA"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..1365)
                     /locus_tag="PLY56_10530"
     CDS             complement(<311..1365)
                     /locus_tag="PLY56_10530"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOJ21960.1"
                     /translation="MLILVLNSGSSSLKFQLFDMADESVLARGTADRIGERGGVDAAI
                     DYRPANREAAKTPVVLPDHATALARVAEILTDPGQGVIASLDAIGAIGHRVVHGGERF
                     SSAALVTPEVAGAIEACAQLAPLHNPPNLVGIRACQKLMPGTPQVAVFDTAFHQRMPR
                     HAYLYAVPYEWYEEYGVRRYGFHGTSHKYVSERAAEWLAREGRNPETLRLITCHLGNG
                     CSMSAVQGGVSLDTSMGFTPAEGLVMGTRAGDIDPANVPYICERTGMTADQVMNALNK
                     KSGLLAMSGYSNDMRDIHARIAEGDARARLALEIFCYRVLKYAGAYAAVMGGVDAIVF
                     TGGIVAGWPTMAQDRIA"
     gene            complement(1428..1991)
                     /locus_tag="PLY56_10535"
     CDS             complement(1428..1991)
                     /locus_tag="PLY56_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOJ21961.1"
                     /translation="MSDDYRGNDVLKLLDRLEEYIEHRPGLMNQAHFVDKDTFFTLTH
                     KIRASLPDEVRQARKVQSDQERIIGDAREEASRIIEDARNQAALLVSQNDIVRQAAER
                     AQALIAQAEQDAARIRAEADAYMREKKRAADDYERDVRRGADDYASEVLDGLHVFVGR
                     ILATIERGQARLEEQRTEEAEREDEEQ"
     gene            complement(2164..2646)
                     /gene="coaD"
                     /locus_tag="PLY56_10540"
     CDS             complement(2164..2646)
                     /gene="coaD"
                     /locus_tag="PLY56_10540"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ21962.1"
                     /translation="MKAIYPGTFDPITNGHLDVIERAARLFTEVIVAVAVNPEKHPFF
                     DMDERVAMIRESTEHLSNVETVAFPGLLVDLAAQLGAQVIVKGLRAVSDFEYEFQMAQ
                     MNRKLNPGVETVFMTTASQYSFLSSSMAKQIVKLGGDISDLVPAPVLRRLRERLASEG
                     "
     gene            complement(2643..3230)
                     /gene="rsmD"
                     /locus_tag="PLY56_10545"
     CDS             complement(2643..3230)
                     /gene="rsmD"
                     /locus_tag="PLY56_10545"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOJ21963.1"
                     /translation="MDGKPDVRIIAGSARGRILKSFKGPGVRPTTDRVRESWFAALGT
                     RVVGSRFLDLYAGTGAVGIEALSRGAARAVLVEKSRQGVAVIRENLRVLGDLPEGAVR
                     VHQGDAVQAVALLEQAGEVFDLVFADPPYEDLEAPQKVLAALGNARGLLAADALVTIQ
                     HSRRVAIPSESGCLVLRSERVYGETTLSSYELGAE"
     gene            complement(3311..>3740)
                     /locus_tag="PLY56_10550"
     CDS             complement(3311..>3740)
                     /locus_tag="PLY56_10550"
                     /inference="COORDINATES: protein motif:HMM:NF040055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21964.1"
                     /translation="GLAQLHQLRGRVGRGTHRSFCLLITDPRSNPDAPGLGDSQGLSD
                     GQRRMKVMVETTDGFQIAEEDLKIRGPGEVYGTRQHGFDAVGQLRIANVLRDARELEE
                     ARAVAQEIIKADPELQHPEHAGLRSALAEADRASAAVVVG"
BASE COUNT          618 a         1329 c         1124 g          659 t
ORIGIN      
        1 gcccacttgc ccatcgcgca ggaagcgaca agagcaacct cccgctcgca ggcaccgtgc
       61 cagcgccagc caggcacggt gggcattctc caattacgcc tggcgggcgc cgacgacctc
      121 cgcggtttcg cgggcgatga ctagctcctc gttggtcgga atgaccagag cgcgcacacc
      181 tgatcccggt gcgctgatct cccgctcgcc ggagcgcact gcgttgcgct cgggatcgaa
      241 gcgcagcccc aagaagctga ggcccgtgca gacccgctcg cgcacgtacg ggtcatgctc
      301 nnnnnnnnnn tcggcgatgc ggtcctgcgc catcgtcggc cagccggcga cgatcccgcc
      361 ggtgaagacg atcgcatcga cgccgcccat cacggccgca taggcacccg cgtatttcag
      421 aacccggtag cagaagatct cgagcgccag gcgcgcgcgt gcgtctccct ccgcgattcg
      481 ggcgtggatg tcgcgcatat cgttgctata gcccgacatg gcgagcagac cgctcttctt
      541 gttgagcgcg ttcatcacct ggtcggcggt cattcccgtg cgttcgcaga tgtacggcac
      601 gttggccggg tcaatgtcgc cagcgcgggt ccccatcacc aggccctcgg ccggcgtgaa
      661 gcccatgctg gtatccagcg acacgcctcc ctgcacggcg ctcatgctgc atccgttgcc
      721 caggtggcag gtgatcaggc ggagcgtctc cgggtttcgg ccttcgcgcg ccagccactc
      781 tgctgcccgc tcgctgacat acttgtgcga ggtgccgtgg aagccgtagc ggcgcacgcc
      841 atactcctca taccactcat aaggcaccgc gtagaggtag gcgtggcggg gcatccgctg
      901 atggaatgcc gtatcgaaca cagccacctg gggtgttcca ggcatcagct tttggcaggc
      961 ccggatgccc accagattcg gcggattgtg gagcggcgcc aactgggcgc acgcctcaat
     1021 cgctcccgcc acctcgggcg tcaccagcgc cgcgctggag aagcgctcgc cgccatgcac
     1081 gacgcggtgg ccgatggcgc cgatcgcgtc gagcgaggcg atcacgcctt gccccggatc
     1141 cgtcagtatc tcggcgacgc gggcgagcgc cgtcgcgtgg tccgggagga ccacgggcgt
     1201 cttggcggct tcccggttcg cgggccgata gtcgatggcg gcatcaaccc cgccccgctc
     1261 gccgatccgg tcggccgtgc cgcgagcaag gaccgactca tccgccatgt caaagagctg
     1321 gaactttaat gatgaactgc ccgaatttag caccagaatt agcacgagtc accctcttta
     1381 cggaagcgca ccctggagcc cgcggcagcc gtgcggtcgg ccagccgtta ctgctcctcg
     1441 tcctcgcgct ccgcctcttc cgtccgctgc tcttccagtc gcgcctgccc ccgctcgatg
     1501 gtggccagga tccgcccgac gaagacgtga agcccatcca ggacttcact cgcgtagtcg
     1561 tcggcgccgc ggcgcacgtc gcgctcgtaa tcatcggccg cgcgcttctt ctcgcgcatg
     1621 taagcatctg cctcggcgcg aatgcgcgcg gcatcctgct ccgcctgcgc gatcagcgct
     1681 tgcgcgcgct ccgcggcttg ccgaacgatg tcgttctggc ttaccagcag cgctgcctgg
     1741 ttgcgcgcgt cctcgatgat ccggctggcc tcttcgcgcg catcgccgat gatacgctct
     1801 tgatcggact gcaccttccg ggcttgacgc acctcgtccg gcagcgacgc gcggatcttg
     1861 tgcgtcaagg tgaagaacgt atccttgtcc acgaagtgcg cctggttcat caggccgggg
     1921 cggtgttcta tatactcttc gaggcgatcc aagagcttga gtacatcatt gcctcgatag
     1981 tcgtcagaca tgagcgacct atcctcctgc cagccgtacc cagaataata gcagagaacc
     2041 tacgcatgtg caagcgcttc gcgtagcgtg tcacgagatc cgcaggcggg ttcggcgcgc
     2101 gcggcaggcc ggccggcaca tatccgcacc gcgagaggcg cgtgatgcct tacggatttc
     2161 ccttcacccc tcgcttgcca gcctctcacg cagccgccgg agcaccggag ctggaaccag
     2221 gtccgagata tcgcccccca gcttgacgat ttgcttggcc atgctggagc tgagaaacga
     2281 gtactggctg gccgtggtca tgaacaccgt ctcgacgccc gggttcagct tccggttcat
     2341 ctgcgccatt tggaactcat attcgaaatc agataccgcg cgcaggccct tgacaatcac
     2401 ctgcgccccc aattgggcag ccagatctac cagcagcccg ggaaaggcaa ccgtctccac
     2461 gttcgagaga tgctcggtac tctcccggat catcgcgacg cgctcgtcca tatcgaagaa
     2521 ggggtgcttc tccgggttga ccgcaaccgc gacgatcacc tccgtgaaga ggcgtgccgc
     2581 gcgctcgatc acatctagat gcccgttggt gatcgggtcg aaggtgcccg ggtagatcgc
     2641 cttcactctg cccccagttc gtaactgctc aatgttgtct ctccgtacac acgttccgag
     2701 cgcaggacca ggcagccgct ctcactcggg atggcgacgc gccgcgagtg ctggatcgtc
     2761 accagtgcat ccgccgcgag aaggccccgc gcgtttccca aggcagccaa caccttctgc
     2821 ggtgcctcca gatcctcgta gggcggatcg gcaaagacca gatcaaaaac ctcgcctgct
     2881 tgctccagga gcgcgaccgc ctgcacggca tcgccctggt gaacccgcac ggcgccctcg
     2941 ggcagatcgc ccagcacccg caggttctcg cggatcacgg cgacgccttg ccggctcttc
     3001 tccaccaaca cggcacgcgc cgcgccccgg ctcagcgcct cgatccccac ggcgccggtg
     3061 cccgcgtaaa gatccaggaa gcgcgagcca acaacgcgcg tgcccagagc cgcaaaccaa
     3121 ctctcgcgca cgcggtctgt tgtcgggcga acgcccggcc ccttgaacga tttcaggatc
     3181 cgccctctgg cacttccggc aatgatgcgc acgtccggct tcccatccac cccagtctgc
     3241 ccgcggctgc gctgtgagcc actccgcgtg gtgaaacagg gaacctgctt cagaaacgcc
     3301 cggcaaaacc ttatcccacg acgacggcgg cggacgcgcg atccgcctcg gcgagggcgc
     3361 tgcgcaggcc ggcgtgttcc ggatgctgca actccgggtc ggccttgata atctcctgtg
     3421 ccaccgcgcg cgcctcttcc agctctcgcg cgtctcggag tacattggcg atgcgcaact
     3481 gccccacggc gtcgaaccca tgctggcggg tgccatacac ctcgcccgga ccgcgtatct
     3541 tcaggtcctc ctccgcgatc tggaagccgt ctgtcgtttc caccatcacc ttcatgcgcc
     3601 gctgcccgtc actgagaccc tggctatcgc ccaggccggg cgcatccggg ttgctgcgcg
     3661 ggtcggtgat gagcaggcag aaagagcggt gcgtgccgcg gcccacgcgc ccacgcaact
     3721 ggtgaagttg cgccaagccg
//
LOCUS       DAOSCV010000405         3740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15597_length_3740_cov_3.335957, whole genome shotgun sequence.
ACCESSION   DAOSCV010000405 DAOSCV010000000
VERSION     DAOSCV010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15597_length_3740_cov_3.335957"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1508)
                     /locus_tag="PLY56_10555"
     CDS             complement(33..1508)
                     /locus_tag="PLY56_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOJ21965.1"
                     /translation="MAILALWIVMMGLLVQREREARRPVDAPPVEWRQPTETVGAAGP
                     AREQWMGVYLNGTKLGYTVTRRQKQDGILRIEAKSHLLLPLQGEMRAVETESLVEVDA
                     AQYMRSFSFTLDSEMQQMRVTGHVEPDRLVLRIESGGTTATRTIPLEKPITVPDALTP
                     ILAGSGRLRANTTHTLDLFDPMALAPAKAMVTIGQPEMIEGVDGPIRVLRAEISYKGF
                     RTTAWVTPEGDTLKEESAMGWTMVRQNREDAMKPPEGDPQTDLVYSAAVPTNRVLKSP
                     RQVKFLRAELTGADFSTLSLATERQQVEAAESGIVEIRREVPEAVRAPRLPVTEPQMK
                     PYLAPDLLIQSDDPAIIETARKIVGRESDSWKAAVRIARWVHREVEKSPTVSVPSALE
                     VLRTRKGDCNEHTVLFVALARAAGIPAEIAVGIVYLNDSFFYHAWPRVWVGQWVSMDP
                     TFGQEVADATHIELVHGGLDRQSEIVRLIGRLRVRIIRAEE"
     gene            1750..1974
                     /locus_tag="PLY56_10560"
     CDS             1750..1974
                     /locus_tag="PLY56_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21966.1"
                     /translation="MAEREGELTVREAGKKGGEIRKQQLGHEGYEEIGRLGGEARKRE
                     LGHEGYEELGQKGGQRVRELIEKGKRAESG"
     gene            2270..3049
                     /locus_tag="PLY56_10565"
     CDS             2270..3049
                     /locus_tag="PLY56_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21967.1"
                     /translation="MLSVHQKGRSAFTLIELLVVIAIIAILAAILFPVFARARENARK
                     SSCQSNLKQLAMGILQYAQDYDERFPYANRTGIPLSSPPPGGFWYQEGGSGSAFWPQM
                     VYPYINNLEVFTCPSGARAYASGPFRGHYGCNELIMPDRFSPTPPLSQAQLTAPASTF
                     LCFDSGAYFLGPSRVTSPSGNFWYIPGTGDILGLDPTQPQGGYVITDALRQDFSSGRH
                     FHGINMAYADGHVKWLKTEQVIQEARKPAPNQYGAWNPAND"
     gene            3198..>3740
                     /locus_tag="PLY56_10570"
     CDS             3198..>3740
                     /locus_tag="PLY56_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21968.1"
                     /translation="MLCVNLRRRSAFTLIELLVVIAIIAILAAILFPVFARARENARK
                     ASCQSNLKQLAMGILQYAQDYDERFPYANRAGLPLDSPPPGGFWYQESGAGTVFWPQL
                     VYPYFNNLGVFTCPSGNRSNTSRPYSGHYGCNRLIMTRHDTTPITLAELAAPASTLLC
                     FDSGAYFMLPSNVTSPSGSFW"
BASE COUNT          674 a         1259 c         1146 g          661 t
ORIGIN      
        1 atgctcgatc tcaatcatcc actccggcac gctcactcct ccgctctgat gatgcgcacg
       61 cgcaggcggc cgatgaggcg cacgatctcc gactgccggt cgaggccgcc atgcaccaat
      121 tcgatgtgcg tggcatcggc cacctcctgg ccgaaggtgg gatccatgct cacccactgg
      181 ccgacccaga cgcgcggcca ggcgtgatag aagaaactat cgttcagata gacgatcccg
      241 acggcgatct cggcggggat gccggccgcg cgcgccagcg ccacgaaaag aacggtgtgc
      301 tcgttgcagt cgcccttgcg ggtgcgtagc acctccagcg ccgagggaac gctcaccgtg
      361 gggctcttct ccacttctcg gtgcacccat cgggcgatgc gcaccgccgc cttccaggag
      421 tcgctttctc ggcccacaat ctttcgcgct gtctcgatga tcgctgggtc gtcagactga
      481 atcaggaggt ctggagcaag gtagggcttc atctgcggtt cggtgaccgg cagccgcggc
      541 gcccgcacgg cttcaggcac ctcccggcga atctcgacaa tccccgactc cgcggcctct
      601 acctgctgtc gctctgtcgc gagcgacagc gtggagaaat cggcaccggt cagctctgcc
      661 cgcaggaact tcacctggcg tgggctcttc agaaccctgt ttgtcggcac agccgccgag
      721 tagacgaggt ccgtttgcgg gtctccctcg ggcggcttca tcgcatcctc gcggttctgg
      781 cggaccatgg tccatcccat cgccgattct tccttcaacg tatcgccctc aggggtcacc
      841 caggcggtcg tacggaagcc cttgtaggag atctccgccc gaagtacgcg gattggccca
      901 tcgacgccct cgatcatctc cggctggccg atggtcacca tcgccttggc aggcgcaagc
      961 gccatcgggt cgaagagatc cagggtgtgg gtggtgttcg cgcgcaggcg cccggaaccc
     1021 gccaggatcg gcgtcagagc atccggcacc gtgatcggct tctccaatgg gatcgtgcgc
     1081 gtggccgtcg tcccgccgct ctcgatgcga agaaccagcc tgtcgggctc gacgtgaccg
     1141 gtgacgcgca tctgctgcat ctcagagtcg agggtgaaac tgaaggagcg catatattgg
     1201 gccgcatcca cctccaccag cgactcggtc tccacggcgc gcatctcgcc ctggagcggc
     1261 aggagaaggt ggctcttcgc ctcgatgcgc aggatgccat cctgcttctg gcggcgggtc
     1321 accgtatagc cgagcttcgt gccattgagg tagaccccca tccattgctc gcgcgcgggt
     1381 cccgcggcgc cgacggtctc ggtcggttgg cgccactcca ccggtggcgc atccaccggc
     1441 cggcgcgcct cgcgctcgcg ctggaccagg aggcccatca tcacgatcca gagcgccaga
     1501 atggccattc cgcgcgctcc tcgaagccac tttgctcgca tcgatcctcc ccggcaggag
     1561 cacctcgccc cgccggattc gaacgccgga tggggcccgt tgccgtctcc ccactcccat
     1621 tctaggggtt gctcgcgagc gtgtcaagca gcggcaccgc cgcgtttggt gcgggaacgc
     1681 cccgggtata ggcgcctcgg aggagccatc tcctcgcctg cagcgttcac gttgcgaaag
     1741 gagatgagga tggcggagcg agaaggcgag ctcaccgtcc gcgaagcggg caagaagggc
     1801 ggcgagatcc gaaagcagca actcgggcac gagggctacg aggagatcgg acgcctggga
     1861 ggcgaggccc gaaagcgaga gctgggccat gaaggctacg aggagctcgg ccagaagggc
     1921 ggccagcgtg tccgcgagct gattgagaaa ggcaagcggg cggagagcgg ataacccctc
     1981 ggaagcaggc tgccataccg ctggagagac aggcgattgt ctctccagat tgatgcccct
     2041 gccccacgat cggatcgcgg gcgagagggg caggcgcggg gagggtgccc ggcccgtggg
     2101 ccaggcaccc tttctcattc tcccgcctgg aagccgaaag taacgccctc ttctcatccg
     2161 gcaggagtcc tccagatcgc cgcgaacgtt acgtgcaacg gtctcaaccc cagcacagga
     2221 ggtgcaacgg tgggtggctc atctgggatg ctatggggag ggcagagaga tgcttagtgt
     2281 tcatcagaag gggcgttccg ccttcacgct gatcgagtta ctcgtcgtca tcgccatcat
     2341 agccattctc gcggcgatcc ttttcccggt cttcgcgcgc gcccgcgaga acgcgcgcaa
     2401 gtcgtcgtgc caaagcaacc tgaagcaact ggcgatgggc atcctgcagt acgcgcagga
     2461 ttacgacgag cggttcccgt atgccaaccg gaccgggatc ccgctctcct cgccgccccc
     2521 cgggggcttc tggtatcagg aagggggatc cggctcggca ttctggccgc agatggtcta
     2581 cccttacatc aacaacctag aagttttcac atgcccttcg ggcgcgcggg cgtacgcatc
     2641 cggcccattc cgtgggcact acgggtgcaa cgaactgatc atgccggacc gtttctcgcc
     2701 cactcctccg ctctcccagg ctcagctcac cgcgcccgcg agcaccttcc tctgcttcga
     2761 cagcggcgcc tacttcctcg gtccctcgcg agtcacttcc ccctcgggca acttctggta
     2821 cattcctggc acgggcgata tcctgggcct cgaccccacc caaccgcagg gcggctacgt
     2881 gatcactgat gcgctgcggc aagacttcag ctcaggccgg catttccacg ggatcaacat
     2941 ggcgtatgcc gacggacacg tgaagtggct caagacggag caagtgatcc aggaagcgcg
     3001 caagcccgcg cccaaccaat acggcgcctg gaacccggcc aacgattagt gcgcaccagg
     3061 taagagagac agggctactc cggcgatata ggcaggagta gcccaactga ccgcgaacgt
     3121 cacgagcaac agtctcactc cggcgcagga ggcacagcga tgggtctcgt ccgggatgct
     3181 acggggagga cagagaaatg ctttgcgtca acctgagaag gcgttccgcc ttcacgctga
     3241 tcgagcttct cgtggtgatc gccatcatcg ccattctggc ggcgatcctc ttcccggtct
     3301 tcgcgcgcgc acgcgagaac gcgcgcaagg cctcgtgcca gagtaacctg aagcaactcg
     3361 cgatgggcat cctgcaatac gcgcaggatt acgacgagcg gttcccgtat gccaaccggg
     3421 ctggactccc gctagattcg ccgccacccg ggggcttctg gtatcaagaa tcgggggccg
     3481 gaaccgtatt ctggccacag ctcgtttacc cttacttcaa caacctgggc gtcttcacgt
     3541 gcccttcagg caaccgctca aacaccagca ggccctattc cgggcattac ggatgtaacc
     3601 gcctgattat gacgcgacat gataccaccc ccatcactct agccgagcta gccgcgccgg
     3661 cgagcactct cctctgcttc gacagcggcg cctacttcat gctgccctcg aatgtcacct
     3721 cgccgtcagg tagcttctgg
//
LOCUS       DAOSCV010000406         3731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15656_length_3731_cov_2.965452, whole genome shotgun sequence.
ACCESSION   DAOSCV010000406 DAOSCV010000000
VERSION     DAOSCV010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15656_length_3731_cov_2.965452"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /gene="cysC"
                     /locus_tag="PLY56_10575"
     CDS             <1..1587
                     /gene="cysC"
                     /locus_tag="PLY56_10575"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOJ21969.1"
                     /translation="TANLAVILIDARNGVVIQSKRHGFIASLLGIPHLVVAVNKMDLV
                     DYSREVFERIRDEYVRFTTKLRIRDVTFIPISALHGDNIVHRSTHMPWYQGPPLLTYL
                     ETVHIASDGNLNDFRFPVQYVLRPNADFRGYCGTITSGVVRVGDEVAVLPSGKRTRIS
                     RILGPNGDTTYAFAPQSITVCLEDAIDVSRGDMLAHPGNLPWMVQDLEAILIWMDNRA
                     LRPGQSYLVKHTTNVVRGECVGLKYRIDPNTLHRQEAATLELNEIGRVHLRMHRPLLC
                     DEYERNRGTGSFILIDPVTHFTAAAGLVIDRCHTHGAVRVSTAAPKKATNVTRHEGRV
                     TPEQRAALLRQRPATLWLTGLSGSGKSTLAYALEERLVAAGHACVVLDGDNVRHGLNR
                     DLGFSAQDRSENIRRVAEVARLFNEAGLIVITSFISPYREDRQMAREIIGGANFIEAF
                     VDAPLQECERRDPKGLYAKARAGQIPEFTGISAPYEAPEAPDLRLESARYSVAELVEQ
                     VYQALEARGCFRPESGEDEK"
     gene            1584..2291
                     /locus_tag="PLY56_10580"
     CDS             1584..2291
                     /locus_tag="PLY56_10580"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOJ21970.1"
                     /translation="MMITVIGRGHSGTRAMSHTLSASGVYMGAKLNVSGDLIPAEELY
                     EACRVMARYVEYKGGLEWDFSRLFTMPIDPAFTRLVESYLSSVLESDAERKGWKLPET
                     TLILPWITRMFPDIYYIYWVRDPRDSIVGAHITDDLADFGVPYERTDDLRRRRAISWR
                     YQYDIMQATPPPARRIHVRFEEFVLKQEETLRRLEDFLGFPMARIPVRPESVGRWRTD
                     TETHDFDFFPREALYEG"
     gene            2397..3080
                     /locus_tag="PLY56_10585"
     CDS             2397..3080
                     /locus_tag="PLY56_10585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ21971.1"
                     /translation="MSHRLVGKVAVVTGSTSGIGAGIAQALAAAGARVVVSGRRSQEG
                     NAVVAAIREAGGEAIFQQTDLRQPEECRRLCLRAAEAFGGLDILVNNAGVFPRMDFEE
                     VTPEFWDQILDINVRAAFFCCQAATPLMRQRGGGSIINIGSVHAFGTRPRMLPYGISK
                     SALYALTMNLARILAPDHIRVNWVTVGWVLTEKEFEVQAGEGTDSEELLKRAETLPMG
                     EFSTVEDVA"
     gene            3182..>3731
                     /locus_tag="PLY56_10590"
     CDS             3182..>3731
                     /locus_tag="PLY56_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012780528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21972.1"
                     /translation="MASKLTRCLALAGIPLAFQAAGSAFAATKEIGNGFADHGVATPI
                     SNHRGIVATVDGEGRNVVLLWLYDHRGGYALLMIDAETGRSKEFPIPFPTGGDGPFAS
                     ILSSANKFYTHFGSHFIEFDPVKGEFTFYHKTVPQMAMGMTEDDNGVIWSVTYPNSGV
                     ASYNPKTGEFKDYGHVYQQNWRQ"
BASE COUNT          659 a         1237 c         1175 g          660 t
ORIGIN      
        1 actgccaacc tcgccgtcat cttgatcgat gcccgcaatg gggtcgtcat tcagtctaag
       61 cgccacggct tcatcgcgtc gctgctcggg attccccatc tcgtagtcgc ggtcaacaag
      121 atggacctgg tcgattactc gcgcgaggtt ttcgagcgga tccgcgacga gtacgtgcgc
      181 ttcactacca aactgcgcat ccgtgatgtc accttcatcc caatcagcgc cctgcacggt
      241 gacaacatcg ttcaccgcag cacgcacatg ccctggtacc aggggccgcc gctgctcacc
      301 tacctggaga cggtccacat cgccagcgat ggcaatctga acgacttccg cttcccggtg
      361 cagtacgtct tgcgtccgaa tgccgacttc cgcggctatt gcggcacgat cacctctggc
      421 gtggtccgcg taggcgatga ggtggcggtg cttccctcgg gcaagcgcac ccgaatctcg
      481 cgcatcctcg ggcccaacgg cgacacgact tatgccttcg cgccgcaatc gatcaccgtc
      541 tgtctggaag acgcgatcga tgtgagccgc ggcgacatgc tagcccatcc gggcaacctg
      601 ccgtggatgg tgcaggatct ggaggcgatc ctcatctgga tggacaaccg cgcgctgcgc
      661 cccggccaga gctatctggt gaagcatacg accaacgtcg tccgcggcga gtgcgtcggg
      721 ctgaagtatc gcatcgatcc gaacacgctg catcgtcagg aggccgcaac actcgagctg
      781 aatgagatcg ggcgcgttca cctgcggatg caccggccgc tcctatgcga tgagtacgag
      841 cgcaaccgcg ggacgggcag cttcatcttg atcgacccgg tgacccactt caccgccgcc
      901 gccgggctcg tgatcgaccg ctgccacacg cacggtgcgg tgcgtgtctc cacggctgct
      961 cccaagaagg cgacgaatgt gacgcggcat gagggccgcg tgacgccgga gcagcgcgcc
     1021 gcgctcctgc gccagcgccc ggccaccctc tggctcacgg ggctcagcgg ctctgggaaa
     1081 tccaccctcg cctacgcgct ggaggagcgc ctcgtcgccg ccggccatgc ctgcgtcgtc
     1141 ttggatggtg acaacgtccg ccatggcctc aaccgcgacc tcggcttctc cgcgcaggat
     1201 cgctcggaaa acatccgccg ggtggcggag gtggcccgcc tcttcaatga ggccggcctc
     1261 attgtcatca cctcctttat ttccccctac cgcgaggacc ggcagatggc ccgggagatt
     1321 attggaggcg cgaacttcat cgaggcattc gtcgatgccc cgctgcaaga gtgtgagcgc
     1381 cgcgatccga agggtctcta cgcgaaggcg cgtgccggcc agatcccgga gtttaccggg
     1441 atctccgcgc cctacgaggc ccccgaggcg ccagatctgc gcctggagag cgcccgatac
     1501 tccgtcgcgg agctcgtcga gcaggtctac caggccttgg aggcacgggg ctgcttccgc
     1561 cccgaatcag gagaagacga aaaatgatga tcacggtcat cggccgcggg cactctggca
     1621 cccgcgccat gtctcacacc ttgagcgcga gcggcgtcta catgggtgcg aaactcaacg
     1681 tatcgggcga cctcattccg gcagaggagc tgtacgaggc gtgccgggtg atggcccgct
     1741 acgtcgagta taagggcggg ttggagtggg acttctcgcg cctcttcacc atgccgatcg
     1801 atccggcgtt cacccgcctg gtcgagtcct atctctcctc ggttctggag agtgacgccg
     1861 agcgcaaggg gtggaagctc ccggagacga cgctgatcct gccctggatc acgcgtatgt
     1921 tccccgacat ctattacatc tactgggtac gcgacccgcg cgattcgatc gtcggggccc
     1981 acatcaccga cgatctggcc gacttcggcg tgccctacga gcgcactgat gacctgcgcc
     2041 ggcgccgcgc catcagctgg cgctaccaat atgacatcat gcaggcgacg ccgccccccg
     2101 cgcgccggat ccacgtgcgc ttcgaggagt tcgtgctgaa gcaggaggag acgctgcgcc
     2161 gcctggagga cttcctcggc ttcccgatgg cgcgaatccc ggttcggcca gagagcgttg
     2221 gccgctggcg caccgacacg gaaacccacg atttcgactt cttccctcgg gaagccctct
     2281 acgagggata ggggctttcg cctctccata cgcgggtttc gctcgcccgt tgctctgccg
     2341 cgcaggtttc ccgcgggcgt ccggcgaatg gatcgcccaa gcaggggagg atcagcatga
     2401 gccatcgtct tgtggggaaa gtggcagtcg tcaccgggtc cacctcgggc atcggtgcag
     2461 gaattgcgca ggcgctcgcg gcagcgggcg cgcgcgtggt ggtgagcggg cggcgctccc
     2521 aggagggaaa cgccgtcgtt gccgccatcc gcgaggcggg cggcgaggcg atcttccagc
     2581 agacggacct gcgccagcct gaggagtgcc ggcgcctctg cctgcgcgcg gccgaagcgt
     2641 tcggcggcct cgatatcctc gtcaacaacg cgggggtctt ccctcggatg gatttcgagg
     2701 aggtgacccc ggagttttgg gaccagatcc tggacatcaa cgtgcgtgcc gcgttcttct
     2761 gttgccaggc agccacgcct ctcatgcgcc agcgtggcgg cgggtcgatc atcaacatcg
     2821 gctcggtgca tgcgtttggc acccggccgc gcatgcttcc ctacgggatc agcaagagcg
     2881 cgctctacgc gctgacgatg aacctggcgc gcatcctggc gccggatcat atccgcgtga
     2941 actgggtgac cgtcggctgg gtgctcaccg aaaaggagtt tgaggtccag gccggcgaag
     3001 gcaccgacag cgaggagctg ctgaagcgcg cggagaccct tccgatgggc gaattctcta
     3061 ccgttgagga tgtcgcctaa gcctgcgtct acctggccgg ggacgagacg gcgcacgtca
     3121 ccggctctaa cctgaacacc ggtgccggac taggagtcca cttctgagag gggcaaggca
     3181 gatggccagc aaattgacac gatgcctggc gttggcgggg attccactcg ccttccaggc
     3241 cgccgggagc gcgtttgcgg cgaccaaaga gataggcaac ggcttcgccg atcacggggt
     3301 agccacgccc atcagcaacc atcggggaat cgtcgccacc gtggatggag agggtcgcaa
     3361 cgttgtcctc ctctggctct acgaccaccg cggcggatac gctctcctga tgatcgacgc
     3421 ggagacggga aggagcaagg agtttcccat ccccttcccc accggtggcg atggtccctt
     3481 cgcctcgatc ctctccagcg ccaacaagtt ctacactcac ttcggcagcc acttcatcga
     3541 gtttgacccc gtgaagggcg agttcacctt ctaccacaag accgtcccgc agatggcgat
     3601 gggaatgacc gaggacgaca acggcgtcat ctggtcagtc acctatccca atagcggggt
     3661 cgcctcttac aacccgaaga cgggcgagtt caaggactac ggccacgtct accagcagaa
     3721 ctggcggcaa t
//
LOCUS       DAOSCV010000407         3723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15700_length_3723_cov_3.714558, whole genome shotgun sequence.
ACCESSION   DAOSCV010000407 DAOSCV010000000
VERSION     DAOSCV010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15700_length_3723_cov_3.714558"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLY56_10595"
     CDS             <1..182
                     /locus_tag="PLY56_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HOJ21973.1"
                     /translation="ARYLVVKERIFTNEGEHWLRYADRGPKAHWVDPESPEVRGLRRG
                     VWLHSLLESDDGDLP"
     gene            199..2553
                     /gene="cas3"
                     /locus_tag="PLY56_10600"
     CDS             199..2553
                     /gene="cas3"
                     /locus_tag="PLY56_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOJ21974.1"
                     /translation="MPDIPALRDVWAKSPVGGAREGESLIAHTAAVLERLAQLYQLRP
                     MLATELEEPDLWHCLYWGTFLHDWGKAAIGFQQQLRPGGSRWGMRHEVLSLAFVDYVA
                     PVGDPSRRWIAAVIVSHHRDADFLEREYAECDPEDDALWYMIREVPEPSLTGLWEWLV
                     RSGEAWFEELGLSKAGVRLPDLPPNPETTSVFQNRAFRSTHQLLGEFLNLVDDLRKDC
                     SDGSARRAAILLRGLNITADHAGSAHTRPFPALPALTSEELLSTWSIQWGQRVTWETL
                     HEHQRQCARSSSSIMLSAPTGSGKTESALLWAASHHRLHPPRLFYVLPYQASMNAMQA
                     RLSALYGEDQVGLQHGKARHALYRRFLEQEYRPEDAGRAARDAANLARLHHPPVRVLS
                     PYQLLKAAYRLKGYEAVLADAYGGLFIGDEIHAYETKRLALIVGLFWHLASHYRARFC
                     LMSATFPRLLREQLEEALPGVTMVSATPETFAAFRRHRVRIQPGVLAEHLDDIRRAAA
                     SGLATLVCCNTVRAAQEVYRALRDKPPGEGWRVELLHGRFNARDRLRKERELMERMGT
                     RVARGDRRVLMVATQVVEVSLDIDFDLLFSEPAPLEALLQRFGRVNRGRRAPLRDVCI
                     FDTPTPGQSIYDERLVARAVELLQREFGEEGAPLDEGLVSAWLDEIYSGEIGDAWREE
                     YARELAEFEIAIMGTLYPFQADKALEDSFYKAFDGIEVLPASLADAYRERADMEPLAA
                     DELLVPVRYGQFARLNRDGKVRPNPDGPPIVDLPYTEEEGLLQG"
     gene            2565..3590
                     /gene="cas1"
                     /locus_tag="PLY56_10605"
     CDS             2565..3590
                     /gene="cas1"
                     /locus_tag="PLY56_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOJ21975.1"
                     /translation="MNSLILDDPGIFLGKHSQRLVLRKGGETLQEVPLFQVGQVIVST
                     QGATLSADAVNACAENGIPITFLGRRGEPMAVLSSPALTATVITRREQLRAYDDERGG
                     ILAYLLASGKIRNQINILRYFSKYRRARDPEVFAAVAERVSFMEALLEELASIPEAQV
                     DSIRSRVLNIEGRAGAQYWEAVGALLAGRIDFPGREHRGATDPVNAMLNYGYGVLYSQ
                     AWRALTLAGLEPFAGFLHVDRPGKPSMVLDFVEEFRAPVVDRTILAMIGKGFRPEMIA
                     EEGQPPRLAPGARKEVATRVLARLEDTHAYEGKRCRLRTILARQAGHLATFLRREGDY
                     KAFVVAW"
BASE COUNT          676 a         1135 c         1250 g          662 t
ORIGIN      
        1 tcgcgcgtta cctggtcgtg aaggagcgta tcttcacgaa cgagggcgag cattggctgc
       61 gctacgcgga tcgtggccca aaggcacact gggtagaccc cgagagccca gaagttcgcg
      121 ggctgcggcg cggcgtgtgg cttcactcgc tgctggaaag tgatgatggc gaccttccct
      181 aagaaggctg gtctgtcatt gcctgacatc cccgcgttgc gcgacgtatg ggccaaaagc
      241 cctgtaggcg gcgcgcgcga gggtgagtcg ctgatcgccc acaccgccgc ggtgctggag
      301 cggctggcgc agctatacca gctgcgtccg atgctggcca ccgagctcga ggagccagat
      361 ctttggcact gtctctattg gggcaccttc ctgcacgatt ggggcaaagc cgccatcggc
      421 ttccagcagc agttgcgccc aggcggcagc cggtggggaa tgcgccatga ggtactctcg
      481 ctggcgttcg tagattacgt tgcaccagta ggagacccga gccggcgctg gatcgcagcc
      541 gtcatagtat cccaccaccg cgacgcggac ttcctggaaa gagagtatgc ggagtgtgac
      601 ccagaggatg atgctctctg gtacatgata cgggaggtac cagagccgtc tctgacagga
      661 ctctgggagt ggttggtgcg gtcgggcgag gcgtggttcg aagaacttgg cttgtccaag
      721 gccggggtac gcctccccga cctccccccc aatcctgaga cgaccagcgt ttttcagaac
      781 cgggctttcc gcagcaccca ccagctcctc ggcgagttcc tgaatcttgt agatgatttg
      841 agaaaggact gcagcgatgg ctcggcgcgg cgcgctgcca ttctcctccg tggtctgaac
      901 atcacagcgg atcacgcagg ttcggcgcat acccggccat ttcccgcgct cccagcactc
      961 accagtgagg agttgctctc cacgtggtcc atccaatggg ggcagagggt tacctgggaa
     1021 acgctgcacg agcatcaacg gcaatgcgct agaagcagca gctcgatcat gctctctgcc
     1081 cccacgggaa gcggcaagac agagtcggcg ctgttgtggg cggcaagcca tcaccggctg
     1141 cacccgccac gtttgtttta cgtactgccc taccaggcta gcatgaacgc gatgcaggcc
     1201 cggctatcgg cactctacgg tgaagatcag gtagggctac agcacggaaa ggccaggcac
     1261 gcgctgtatc ggcgattcct cgaacaggag tatcgcccgg aggatgcagg gcgggcggcc
     1321 agggacgcag cgaatctggc gagactacat caccctccgg tccgcgtcct cagcccatac
     1381 cagctactga aggcggcgta tcgtctgaag ggctacgagg ccgtgctggc ggatgcctac
     1441 ggggggctct tcatcggtga tgagatccac gcttacgaaa cgaaacggct cgcgctgatc
     1501 gtaggcttgt tttggcacct tgcctctcac tatcgggcac gtttctgcct gatgtccgcc
     1561 accttcccac ggctcctgcg agagcaactg gaggaagcgc tgccaggggt gacaatggta
     1621 tcagcgaccc cggaaacgtt cgccgcattt cggcgccatc gcgtgcgcat ccaaccgggc
     1681 gtactcgccg agcatctgga tgatattcgg agagcagccg catccggatt ggcgacgctg
     1741 gtctgctgca atacagtgcg agcagcgcag gaggtgtacc gggcgctccg cgacaagccg
     1801 ccgggcgaag gctggcgcgt ggagctcctt cacgggcggt tcaacgcaag agaccgcctg
     1861 cgcaaagagc gtgagttgat ggagaggatg ggcacccgcg tggctagggg tgaccgccgg
     1921 gtgctcatgg ttgcgaccca ggtagtggaa gtcagtctcg acatcgactt cgacctgctg
     1981 ttcagcgagc ctgctcctct tgaggctcta ctacagcgat ttggccgggt caaccgaggt
     2041 cgccgtgcac cgttgcgtga cgtctgcatc ttcgatacgc cgacacccgg ccaaagcatc
     2101 tatgacgagc gattggtggc gcgcgccgtc gagctcttgc agcgcgaatt tggcgaggaa
     2161 ggcgccccct tggatgaagg cctggtatca gcttggctgg atgagatcta ctccggcgag
     2221 atcggagatg cgtggcgcga ggagtacgcg cgcgagctgg cagagtttga gatagccatt
     2281 atgggcacgc tctatccctt ccaggccgat aaggcgctgg aggacagctt ctacaaagca
     2341 ttcgatggta tcgaggtgct cccagcgtct ctggcggatg catatcgcga gcgtgccgac
     2401 atggaacccc tggccgcaga cgagctgctg gtgcctgtgc gctatgggca attcgccagg
     2461 ctgaatcggg atggcaaggt ccggccaaac cctgacggac ctccgatcgt ggatctaccg
     2521 tacacggaag aggaaggact gcttcagggc taaggaggat gcagatgaac tctctcatcc
     2581 ttgatgaccc gggcatcttc ctggggaagc actcgcagcg gctggttctg cgcaaggggg
     2641 gcgagacgct gcaggaggtg cccctgttcc aggtgggaca ggtgatcgtc agcacgcagg
     2701 gcgcgacgct gtcagccgac gcggtgaacg catgcgccga gaacgggatt ccgatcacgt
     2761 ttctcggccg gcgaggagag ccgatggccg tgctctcctc gccggcgctc accgcgaccg
     2821 tcatcacgcg gcgcgagcag ctgcgcgcct acgatgacga gcgcggcggc atcctcgcgt
     2881 acctgctcgc ctcaggcaag atccggaacc agatcaacat cctgcgctac ttctccaagt
     2941 accggcgcgc gcgcgatccg gaggtgttcg ccgcggtcgc cgagcgcgtc tccttcatgg
     3001 aggcgctgct cgaggagctg gccagcattc cagaggcgca ggtagattcc atccgctccc
     3061 gcgtgctcaa catcgagggg cgcgcggggg cacagtattg ggaagccgtg ggcgcactcc
     3121 tcgccgggcg gattgacttc ccggggcgcg agcaccgggg cgcgaccgac ccggtgaacg
     3181 ccatgctcaa ctacgggtat ggcgtcctct actcgcaggc gtggcgcgcg ctcaccctgg
     3241 ctggattgga acctttcgcg ggtttcctgc acgtggaccg cccgggaaag ccgagcatgg
     3301 tcctcgattt cgtggaggag ttccgtgctc cggtagtcga ccgcacgatc ctggcgatga
     3361 ttggcaaagg cttccgaccg gagatgatcg ccgaggaggg ccagccgccg cggctggcgc
     3421 caggagcacg caaggaggtg gcgacgcgcg tcctggcacg cctggaggat acgcacgcat
     3481 acgaggggaa gcggtgccgg ctgaggacga tcctggcccg gcaggccggc cacctggcca
     3541 ccttcctgag gcgcgagggc gactacaagg cgttcgtggt cgcctggtaa agggactgcc
     3601 tgcccgattc acccgggcgc ggcgggaagg ctgccccgga ggcctggcac tcgcgtcccc
     3661 taccccgggg tggcatcatg gtgacgctgg tgatctacga cgtcgtggac gaccgggtgc
     3721 gcg
//
LOCUS       DAOSCV010000408         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15760_length_3715_cov_3.390710, whole genome shotgun sequence.
ACCESSION   DAOSCV010000408 DAOSCV010000000
VERSION     DAOSCV010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15760_length_3715_cov_3.390710"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="PLY56_10610"
     CDS             complement(<1..1605)
                     /locus_tag="PLY56_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21976.1"
                     /translation="MAVETKTPTPAKAPPARLWILALLLIPGGAFLGTYSYIVAQAIQ
                     YTQTSLLRGPVILLTGIIAGNALVRVIGRRAGLTQSELVLLYIMLTLSTAIGGVGMVQ
                     FLVPTLAAPFYFATPENGWGEFLPLIPSGFAVRDPEVLKGFFQGNSTLYDPRVLRAWA
                     GPVLLWSVFLMVLILSMLCACVLVRQRWTDHERLSFPLVLLPMQMATGGPAFWRNRIM
                     WAGFAIAGILESINYINWLYPSFPYVQLKPYSISQFITQRPWSGIAPLWLAFYPFAIG
                     IGFLLSLDISFSCWFTYLLIKSENVVAIMLGFRDPGAGVALARAPYIAEQGAGAYLGM
                     GLFILWSMRRHLRDRLFSPKEASDGGEVIPYRLALIGLGVCFAAMAIFFWVMGLPLWA
                     GTALLVLYLLSILTVSRLVAEAGTGWTLGSDLNAHGVINVAAGTGALQPKAATTFASL
                     LWFDLDYRDSPMPHQLEGTRMASLAGITPRLLLAAILVAMVVGIAAAFWSHLDIYYRY
                     GAASARTRPWVTSMGQQPWVQLGRWLR"
     gene            complement(1735..2718)
                     /locus_tag="PLY56_10615"
     CDS             complement(1735..2718)
                     /locus_tag="PLY56_10615"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ21977.1"
                     /translation="MSEWNRHARARTRGVVINPEDLSFREWPERAKEAGLTTIALHDS
                     RAGATIAPILEREEGQRFLEGCQRLGLAVEYELHAVCDLLPRSLFETNPELFRMNDAG
                     ERTPDANLCVHSEQALEIVAEAAVRVAKTLRPTTGRYFYWGDDAAPACHCPRCRDLTP
                     ADQALLVENRILAALRTLDPHATLAHLTYAYTLIPPSQVRPAPGLFLEFAPGGLHCIE
                     KVEGDASLGTTDRSVKERYFELLDANLEIFDAGQAQVLEYWLDVSAFAGWRKPVTRAL
                     DWERRRPAFALDLEAYRSRGIQQFTTFACYADADYVSAFGELPLAEYASAL"
     gene            complement(2830..3594)
                     /locus_tag="PLY56_10620"
     CDS             complement(2830..3594)
                     /locus_tag="PLY56_10620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ21978.1"
                     /translation="MNKYHEANRKGWDAVSPIWQAGIEQHGVWHRCHREPELVLRPEE
                     RRYLGDVAGQEICVLGSGDNLVVFALAGLGARVTSVDISQVQLDTAARRARELDLDVR
                     FLRADVTDLSAIPDASFDGVYTGGHVAVWVSDLRRYYAEAGRILRPGGLFMVNEYHPF
                     RRIWRWGATPLELEASYFVRGPFQTDRSDEVDGAAPGSIPSYEFHWTVSDYVSAMLAA
                     GCRILALEEMGDGLEEWENAPLEGLPLCLLLVGRKE"
BASE COUNT          690 a         1156 c         1277 g          592 t
ORIGIN      
        1 gcgcagccag cggccgagtt gaacccacgg ttgctggccc atgctggtga cccaggggcg
       61 ggtgcgcgcg ctggcggcgc catagcgata gtaaatatcg aggtgagacc aaaaagcggc
      121 ggcgatgccc accaccatgg ccaccaggat ggcggccagg aggagacggg gcgtgatgcc
      181 ggcgagggat gccatgcgcg tcccctcgag ctggtgcggc atcgggctgt cgcgatagtc
      241 gaggtcgaac cacagcaggc tggcgaaagt ggtggccgcc tttggctgca gcgcgccggt
      301 gccggcagcc acattgatca ccccgtgcgc gttcaggtcg gagccgagcg tccacccggt
      361 gccagcttcc gcgaccaggc gcgacacggt caggatagag aggagataga ggaccaggag
      421 cgccgtgcca gcccagaggg ggaggcccat aacccagaag aagatcgcca tggcggcaaa
      481 acacacgccc aatccgatca aggccagccg gtaggggatc acctcgccgc cgtccgatgc
      541 ctctttcggc gagaacaggc gatcccgcag gtggcgacgc atgctccaca ggatgaagag
      601 acccatgccg aggtaggctc cggcaccctg ctccgcgata tagggggcgc gtgccagggc
      661 gacgcccgct ccaggatccc ggaagccaag catgatggcg acgacgttct cgctcttgat
      721 caggagatac gtgaaccagc agctaaacga gatatccagg ctgagcagga agccgatgcc
      781 gatggcaaat gggtagaacg cgagccacag gggcgcgatg ccgctccagg gccgctgggt
      841 gatgaactgc gagatggagt agggcttcag ctgcacgtag ggaaaggagg gatagagcca
      901 gttgatgtag ttgatgctct ccaatatccc cgcgatggca aagccggccc acatgatccg
      961 gttgcgccag aatgccgggc ctcccgtggc catctgcatt ggcaagagga ctagcgggaa
     1021 ggagagacgc tcgtggtcgg tccatcgctg gcgcacgagc acgcacgcgc agagcatgga
     1081 gaggatgagg accatcagga agaccgacca aaggagcacc gggccggccc acgcgcgcag
     1141 cacccgaggg tcgtagagcg tggagttgcc ctggaagaag cctttgagca cctccgggtc
     1201 gcgcacggcg aacccggagg ggatgagggg cagaaactct ccccagccgt tctccggggt
     1261 ggcaaagtag aagggcgccg ccagtgttgg caccaggaac tgaaccatgc ccacgccacc
     1321 gattgcggtg ctcagcgtga gcatgatgta gaggaggacc agctcgcttt gggtgaggcc
     1381 ggcgcgcctc ccgatcacgc gaaccagggc gttcccggcg atgatgccgg tgagaaggat
     1441 caccggtccg cgcaagagtg acgtctgggt atactgtatc gcctgggcga ctatataact
     1501 gtaagtaccc agaaaagcgc ccccgggaat gagaaggagc gccaggatcc acaggcgcgc
     1561 tgggggcgcc ttcgccggag tcggagtctt cgtctctact gccatggcac gacacaattc
     1621 ccgatggtat tgggcgaatc ctgccagaga taaagcgctc agcgaatcag cgattgcccg
     1681 cggggcaacc ggcgggggaa gatgcatccg tggcttgggg aagcctatcg ctcatcacag
     1741 cgccgaagca tactctgcga gtggaagctc gccaaacgcg cttacgtagt ctgcgtcggc
     1801 atagcaggca aaggtggtga actgctggat gccacgcgac cggtaggcct ccaggtccag
     1861 ggcgaacgcg ggccgccgcc gctcccaatc gagggcgcgc gtgaccggct tgcgccagcc
     1921 cgcaaacgcg gagacgtcca gccaatactc cagcacctgc gcctgcccgg cgtcaaagat
     1981 ctctaggttg gcgtcgagca gttcgaagta gcgctccttg accgagcggt cggttgttcc
     2041 cagcgatgcg tctccctcca ccttctcgat gcagtggagg ccgcccggcg cgaactcgag
     2101 gaagagcccc ggcgcgggcc gtacctggct cgggggaatc agggtgtagg cgtaggtgag
     2161 atgcgccagc gtggcgtggg gatcgagcgt gcgcagcgcg gcgaggatgc ggttctccac
     2221 gaggagcgcc tgatccgcgg gggtgaggtc acggcagcgc gggcagtggc aggcaggcgc
     2281 ggcatcatcg ccccagtaga agtagcgccc ggtggtcgga cgcagggtct tggcgacccg
     2341 gacggcggct tcggccacga tctcgagcgc ctgttcggag tggacgcaga ggttggcgtc
     2401 cggggtgcgc tctcccgcgt cgttcatccg gaagagctcg gggttcgtct caaaaagcga
     2461 gcgcggcaag aggtcgcata ccgcgtgcag ctcatactcc accgcgaggc cgaggcgctg
     2521 gcatccctcc agaaaacgct ggccttcttc ccgctcgagg atgggggcga tggttgcccc
     2581 cgcgcgcgaa tcgtggaggg caatggtggt gagccccgcc tctttcgcgc gctccggcca
     2641 ctcgcgaaag ctgaggtcct cggggttgat cacgaccccg cgcgtgcgtg cgcgtgcgtg
     2701 tctgttccat tcactcatgc cgcttccagt tcccttcttc ggtggccgat ctggcatcgg
     2761 cttggcgggt gagttcgacc ctcacgggct gcgctcctgg cgcgctggca tcagggctgg
     2821 ccaaccctct cactccttgc ggcctacgag gagcaggcag agtggcagcc cctccaacgg
     2881 cgcattctcc cattcctcca ggccatcgcc catctcctcc agggcgagga tgcggcagcc
     2941 ggctgcgagc atcgcggaga cgtagtcgct caccgtccaa tggaactcgt agctcgggat
     3001 cgatccggga gccgcgccgt ccacttcgtc ggagcggtcg gtctggaacg ggccgcgcac
     3061 gaagtaggat gcctctagtt ccaggggggt ggccccccat ctccagatgc gtcggaaggg
     3121 atggtactcg ttgaccatga agagcccgcc tggtctcagg atgcgtccgg cctctgcgta
     3181 atagcggcgt aggtcggaga cccacacggc cacatgcccg cccgtgtaga ccccatcgaa
     3241 gctggcatcg gggatggcgc tcagatcggt cacatcggct cggaggaagc gcacatccag
     3301 gtcgagttct cgcgcgcggc gagcggcggt gtcgagttgc acttgcgaga tgtcgaccga
     3361 cgtgacgcgc gcgccaaggc ccgccagggc gaagacgacc agattatcgc cgctgccgag
     3421 aacgcagatc tcctgcccgg ctacatcccc caggtagcgg cgctcctcag gccggaggac
     3481 cagctccggc tcgcggtggc atcgatgcca cacgccgtgt tgctcgatgc cggcttgcca
     3541 tatgggcgag acggcatccc atcctttgcg gttggcttcg tgatacttgt tcactgctcc
     3601 tccgtttcga tttctaccga gggcgcaact tctctcgcct gatagcctcg gagttctgcc
     3661 tccccgcggt cgcatcctgt caacgagcgt ggcgtgcgca cgtgcgagag agggg
//
LOCUS       DAOSCV010000409         3709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15793_length_3709_cov_3.628626, whole genome shotgun sequence.
ACCESSION   DAOSCV010000409 DAOSCV010000000
VERSION     DAOSCV010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15793_length_3709_cov_3.628626"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLY56_10625"
     CDS             complement(<1..495)
                     /locus_tag="PLY56_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21979.1"
                     /translation="MVPREKALEGAEMVVRAEEFARTNLRVDRTECGDHQPVLLDQGE
                     YPNFAEYDFRVDEAGLFELHALYAADQSRPVDVYVDGRRVVRAGLAGTTGSFKASSAR
                     WERQGTLFLTAGEHTLRLERTAAVPHIAALALRRDPRQIAGATLEALPSEAYIRDDFL
                     VQEWM"
     gene            652..990
                     /locus_tag="PLY56_10630"
     CDS             652..990
                     /locus_tag="PLY56_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HOJ21980.1"
                     /translation="MGHYVDILLEFVTRFAEVGAAIVILTGITQTLYRWARHWRWGRI
                     SPDDRISLGRSIVLGLDFLVASDVLMTAIAPTWSHLGQVASVILLRLLLAYFLELELR
                     RFMESREGSA"
     gene            complement(1037..1585)
                     /locus_tag="PLY56_10635"
     CDS             complement(1037..1585)
                     /locus_tag="PLY56_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005962199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21981.1"
                     /translation="MIGCQDFCGHYDWTFEYLHREYGDEALHDYWEQAISRDSQRHAR
                     ALISDKGFQGMSEYWGHTLVEEEAGYIATEAEGLYRIDMYRCPSLGFLLEHGLRHYHD
                     YCEHCMGWIAPIMAENGFSIDHAHNHRGQCWWEIRRAADSKGPSAPGEVAGDHDVRLR
                     EDWPAGEEHLFLRSRRAVSGPA"
     gene            complement(1582..2010)
                     /locus_tag="PLY56_10640"
     CDS             complement(1582..2010)
                     /locus_tag="PLY56_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21982.1"
                     /translation="MAAPDFYFAINATFRFIEERWGEEALNRYWEAMGRGYFAPLSAR
                     FRTGGLAAVEAYWRDFFAEEPGGEVDVRREGGRVAIRVRRCPAIHHLRTHGREILPRY
                     CQHCRVVSGVIAENAGLSFAMEGGDGACEQWWMEEGEGKR"
     gene            complement(1997..3112)
                     /locus_tag="PLY56_10645"
     CDS             complement(1997..3112)
                     /locus_tag="PLY56_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ21983.1"
                     /translation="MSSSVSAPARVLVVDDDPELLEQLQLATRSEGWVWAAAVGVRAA
                     LAVATGHQPHGVVVADALPDGNGTDLIRELRRQHPGGLSIRMAGQSDLENAISMTGTR
                     AFAYLSRPFPLVQLIALIRSAVEMARLREELQGKTFRTARMQAVLDTIRLMQDCINNP
                     LQGIMANAELMQIVEPSLPPRAREILGKMTRCCEAIGDNIHRLARVLSDCVLSGEGNL
                     ASNTDGLLGVLRGCHARGCRARVAPGPVPQAKTPGEERRREPRYCCTTLMLSAPVDGA
                     TRWVLVDNISTGGVGLRLLSGDLLPSRFHARLWNLESRESIPVDVEVRWVRAGDPTPA
                     GLRFVKEDAHEFLKGMLSRIHDPSGAKETREACNGCS"
     gene            3526..>3709
                     /locus_tag="PLY56_10650"
     CDS             3526..>3709
                     /locus_tag="PLY56_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21984.1"
                     /translation="MLVLLLLAMVPPAWSLPTGEQVLRHVREARSGIRDYTVDVSIHV
                     EMEGVRVPDATIRVFHK"
BASE COUNT          674 a         1271 c         1125 g          639 t
ORIGIN      
        1 catccactcc tgaaccagga aatcatcgcg gatgtacgct tccgagggca gcgcttccag
       61 ggttgcgccg gcaatctggc ggggatcgcg gcgcaaggcg agcgcggcaa tgtgcgggac
      121 ggccgcggtg cgctccaggc gaagcgtgtg ctcgccggct gtcaggaaga gggttccctg
      181 ccgctcccag cgcgccgagg acgccttgaa gcttcccgtc gtcccggcca ggccggcgcg
      241 caccacccgg cgaccatcga cgtagacatc taccgggcgc gattggtcgg cggcatacag
      301 ggcatggagc tcaaacagcc ctgcttcatc cacgcggaaa tcgtattcgg cgaagttcgg
      361 atactccccc tggtcgagga gcaccggttg gtgatcccca cattccgtgc gatccacgcg
      421 gaggttcgta cgcgcgaact cctcggcgcg cacgaccatc tccgcccctt cgagcgcctt
      481 ctcccgcggc accatctcgc ctccatccac ccatcttgcc aaaggcgacg ggcagcccag
      541 cggtgctgcc cgtgacacca gggggttcgt cgcgagcgcg cgctttccct ttggggttcg
      601 tcttccgcgc agccgggtag actcccgccg acagggagag acgccagggc gatggggcat
      661 tatgtggata tcctgctgga gttcgtgact cgcttcgcag aagtcggagc ggcgatcgtc
      721 atcctgacag gcatcaccca aacgctctac cgatgggcgc ggcactggag atggggaagg
      781 atctcccccg acgaccgcat cagcctcggc aggtcaatcg tcctcgggct cgatttcctg
      841 gtggcctcgg atgtcctgat gaccgcaatt gcacccacct ggagccacct gggccaggtg
      901 gccagcgtga ttctcctccg gcttctcctg gcctacttcc tcgaattgga gttgcgccgt
      961 ttcatggaaa gccgcgaggg atcggcctga tgccttccca cccaagccga cgccgggctc
     1021 ggataccgcg gcaggatcac gcgggcccgg agacggcacg cctggaacgg agaaagaggt
     1081 gctcctcgcc cgcgggccaa tcctcacgga ggcgcacgtc gtgatccccc gccacttcgc
     1141 cgggcgcgga cggccccttc gaatcggcag cgcggcggat ctcccaccag cactggccgc
     1201 ggtggttatg ggcatggtcg atggagaagc cgttctcggc catgatcggc gcgatccacc
     1261 ccatgcagtg ctcgcaatag tcgtggtagt gccgcagccc gtgctccagg agaaacccaa
     1321 gcgaggggca ccgatacata tcgatgcggt acaggccttc cgcctcggtg gcgatgtagc
     1381 ccgcctcttc ttcgacgagg gtatgccccc aatactcgga catcccctgg aagcccttgt
     1441 cgctgatgag ggcgcgcgcg tgccgttgcg aatcgcgaga gattgcctgc tcccagtagt
     1501 catgcaacgc ctcgtcgcca tactcccggt ggaggtactc gaaggtccaa tcgtagtggc
     1561 cgcagaaatc ctggcatccg atcatcgctt cccctcccct tcctccatcc accactgctc
     1621 gcaggcgccg tctcccccct ccatcgcgaa agagagcccg gcgttctcgg caatcacccc
     1681 agagacgacg cggcagtgct ggcagtaccg gggcagaatc tcgcgcccgt gggtacgcag
     1741 gtggtgaatg gccgggcacc ttcgcacgcg aatggccacg cggccgcctt cacgccgcac
     1801 atccacctcg ccgccaggct cctcggcgaa gaaatctcgc caatacgcct caaccgccgc
     1861 caaccctccg gtgcgaaagc gagcagagag gggcgcgaag taaccacggc ccatcgcctc
     1921 ccagtagcgg ttcagagcct cttcacccca ccgttcctcg atgaagcgaa acgtcgcgtt
     1981 gatggcgaag tagaaatcag gagcagccat tacatgcctc cctcgtctcc ttggcgcccg
     2041 atggatcatg gatgcgcgac agcatgcctt tcagaaactc gtgggcatcc tccttcacaa
     2101 aacgcaaacc agcgggcgta ggatcgccgg ccctcaccca acgcacttcc acatcaaccg
     2161 gtatggattc gcgcgattca aggttccaaa ggcgtgcatg aaatctcgag gggagcaaat
     2221 cgccgctcag caagcgcagc ccgacgccac cagtgctgat gttatctacc aaaacccatc
     2281 gggtggcgcc atcaaccggt gccgacagca tgagggtggt gcagcagtac ctgggttcgc
     2341 ggcgtcgctc ttcgccgggc gtcttggcct ggggcactgg tccgggtgca acgcgggccc
     2401 tgcagccacg agcatgacac ccgcggagga cccccagcaa gccatccgtg tttgatgcca
     2461 ggttcccttc gcccgagagc acgcagtcgg agagcactcg cgctagccgg tggatattat
     2521 ccccaatagc ctcgcagcac cgcgtcattt tgccgaggat ctccctcgcg cgtggcggga
     2581 ggctcggctc cacgatctgc atcagttcgg cattcgccat gattccctgc aacgggttgt
     2641 tgatgcaatc ctgcatcagg cgtattgtat caagaaccgc ctgcattcgg gcggtacgga
     2701 aggtctttcc ctgaagctcc tcgcgcagcc gggccatctc cacggcagag cgaatcaacg
     2761 cgatgagctg cactagcggg aaaggcctgc ttagataggc gaaggcgcgc gtgccggtca
     2821 tgctgatggc gttctccaga tccgactgcc cagccatccg gatcgagagc ccgcccggat
     2881 gctggcgccg caactcgcgg atcaggtcgg tgccattccc atccggcagg gcatcggcca
     2941 caaccacccc atgtggctgg tgcccggtgg cgacggccag cgccgcgcga actccaacgg
     3001 cagcggccca gacccatcct tccgagcgag tcgccagctg caactgctct aacagctcgg
     3061 gatcatcgtc gacgaccagc acgcgcgccg gcgctgaaac agaggatgac atctccatga
     3121 cctccataaa gcaggcaggt ccactgcctg ccgtcttgag gcgcggccgc gcgtaactgc
     3181 cacgcaggct ctcagcactc aatggatccg ttcgccaacc gacgctcaag ccctgcccca
     3241 ctctccctgt gcacaagagg cacctgcacc tggagcagtg ttgcaggggg cgcacttcgg
     3301 tggtatactg tgcctatgcg aagcaagacc tgctctgcgt gccgggcaaa agagcgccag
     3361 gcgaagcctc gaccgaatcc gggaggggcc acgccgcgca tgcaccctcg attcacggcg
     3421 aactgcgtgg ccggtgtcga taccggccat gctcggggcc gcctttggcc agtccctcga
     3481 ttcgacagaa cgcgaggcgc tctcgcgggg gccaggcttc ccgttatgct agtgcttctt
     3541 ctccttgcca tggtgccgcc ggcatggtcc ctgcccacgg gcgagcaggt gttgcgccac
     3601 gttcgcgaag cccgttccgg gatccgcgac tacaccgtgg atgtgagcat ccatgtggaa
     3661 atggaagggg tccgggtgcc agacgccacg atccgcgtct tccataagg
//
LOCUS       DAOSCV010000410         3702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15831_length_3702_cov_2.831368, whole genome shotgun sequence.
ACCESSION   DAOSCV010000410 DAOSCV010000000
VERSION     DAOSCV010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15831_length_3702_cov_2.831368"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2158
                     /locus_tag="PLY56_10655"
     CDS             <1..2158
                     /locus_tag="PLY56_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOJ21985.1"
                     /translation="QLAAGDLDGDGFDDLYVLGGGQARVYWGGPDGLAPDRCTLVQAT
                     VPPAPEAEAEELAPETEAVRAIAPVPAIIVLNGTPHLFLPGEEHALLAPVLPGREIGR
                     PLLFRCPHAIAVAAGDVNGDGFLDLVFASRGHHDGQECSWVYWGSARGFDEVRRTPLP
                     SDGACDVAVGDLTGNGCADIALCQDRTPDSFTYHSLLYLASREGISPEPVWLESHDAR
                     RVFIARTQPAERPQVILVNHRARRSGGDVDPVIYYGGPDGFLPEHRRALKGRDCVDAV
                     CCDIDDDGHVEIITANCSENAIDLDPGSFLFKGGPDGYPYEPATVFPTARAHGVCCAD
                     LNHDGYLDLVFVGFRNPELLIFYGGPEGYDLENPHRIRMEADGEVYQEPRWVYAADLN
                     NDGWIDLFVPQIASDRSFILWNGPEGFSMERRQMLSVFHAACAQAADLTGNGYLDLVI
                     GGHTPSLEGPHDSFIYIYWNGPEGLREDRRAQLPALGAEALAIADFNRDGHPDIFVAN
                     YHDGRTRDLDSFIYWGGPGGRFSAQRRSRLFMHSAAGAVACDFNEDGWVDLAVGYHKV
                     DNDHVGHSGVWWNGPEGFSPTRVTPLPTEGPHGLVRVGASNQADGGPEEYYTSAPFPL
                     PEGAVLDHLEWEATVPPKCWVRAQIRSAASREALQTTPWQGPAGEGSWYERGGEIPPH
                     HPAGRWVQYRLALGARNGCGTPRVTTVRLVWRDGAE"
     gene            complement(2189..3367)
                     /locus_tag="PLY56_10660"
     CDS             complement(2189..3367)
                     /locus_tag="PLY56_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ21986.1"
                     /translation="MNPIRLALVGTGGRGQIYVDVVKRMPAGRVVWAAMCDRNEGVLN
                     DFCDRNGLTAPRILSVEELVRRNDIDAVLICTPDSIHREPAVACFEAGFHCLVEKPLA
                     TTPEDARAICRAARRSGKLLHLGFVFRYDPCILKLRELVAGGAIGRVIACITHDAIGW
                     FHGSTYMRRWNRFRKFSGDMLLHKGCHTFDVINFITGAYPARVAAFGGTEVFQPREGA
                     ASHCHACRLTDDCIYYTDQGPEYRERFYNTAGPQVLPEDMCVYNNEKDSTDTVSLTAE
                     FTNGMRLSYTLSMVSPQGRRRMTFIGTRGEIRCDMEEYRIEYWPLPDRPVDVIEVPRP
                     TGTGHQHHDRALFSHFLERLEQGDDPERGILDAYMSGAIPFAALESLQKGEVVAIPSP
                     "
BASE COUNT          588 a         1287 c         1215 g          612 t
ORIGIN      
        1 ccagctggcg gcgggcgatt tggatggcga cggcttcgat gacctctacg tgctgggcgg
       61 gggccaggca cgggtctact ggggtggccc ggatggcctg gcgcccgatc gatgcacgct
      121 tgtccaagct acggttccgc ccgcgcccga ggcggaggcg gaagagctcg cccctgaaac
      181 cgaggcggtg cgcgccatcg ccccggtgcc ggcgatcatc gtcctgaacg gcacgcccca
      241 cctctttctc cccggcgagg agcacgcgct gctcgcgcca gtgctgcccg gccgcgagat
      301 cggccgcccc ctgctcttcc ggtgcccgca tgccatcgcg gttgccgcgg gcgacgtaaa
      361 tggcgatggg ttcctcgacc tggtctttgc ctctcgtggc catcacgacg gccaggagtg
      421 cagctgggtc tattggggca gcgcccgcgg cttcgatgag gtccgccgga cgcctctccc
      481 cagcgatggt gcctgcgacg tggcggtggg cgacctcacc ggaaatgggt gcgccgatat
      541 cgccctttgc caggaccgca ctcccgactc gttcacgtac cactcgctgc tctacctggc
      601 gagcagggag ggaatctcgc ccgagccggt ctggctggag agccacgacg cacgccgcgt
      661 cttcatcgcc cgcacgcagc cggccgaacg cccacaggtg attctggtca accaccgggc
      721 gcggcgctcc gggggcgacg tcgacccggt gatctactac ggcggcccgg atggcttcct
      781 gccggagcac cggcgcgccc tcaaaggccg cgactgcgtg gacgccgtct gctgcgacat
      841 cgatgacgac ggccacgtgg agatcatcac ggccaactgc tccgagaacg ctatcgacct
      901 cgatcccggc tcatttctct tcaagggcgg gccggacggc tacccctacg agccagcgac
      961 tgtcttccca accgcccgcg cccacggcgt ctgttgcgca gacctgaacc acgatggcta
     1021 cctggacctg gtcttcgtcg gcttcagaaa cccggagctc ctgatcttct acggcgggcc
     1081 ggaggggtat gacctggaga acccgcaccg gatccggatg gaggccgatg gcgaagttta
     1141 ccaggagccg cgctgggtgt acgcggccga cctgaacaac gatggctgga tcgacctttt
     1201 cgtgccgcaa atcgccagcg accgcagctt catcctctgg aacggccccg agggcttcag
     1261 catggagcgc cggcagatgc tctcggtctt ccacgccgcc tgcgcccagg cagccgacct
     1321 tacaggcaac ggctatctcg acctggtgat cggcgggcac accccctcgc tggaagggcc
     1381 gcacgactcc ttcatctaca tctactggaa cggccccgag ggcctgcgcg aggaccgccg
     1441 cgcgcagctc cccgccctgg gcgccgaggc gctcgcgatt gccgatttca accgtgacgg
     1501 ccatcccgac attttcgtcg ccaactacca cgacggtcgg acgcgcgacc tcgattcgtt
     1561 catctactgg ggcggtccgg gtggccgctt cagcgcgcag aggcggtcgc gcctcttcat
     1621 gcactccgcc gcgggcgctg tcgcctgcga cttcaacgag gatggttggg tggacctcgc
     1681 ggtcggctac cacaaggtcg acaacgacca cgtgggccat tctggggtat ggtggaacgg
     1741 ccccgagggt ttcagcccca cgcgcgtcac gcccctgccc acggagggcc cgcacggttt
     1801 ggtgcgcgtg ggtgccagca accaggcgga tggagggccg gaggagtact acacctcggc
     1861 acccttcccc ctgccggagg gagcggtgct cgaccacctg gagtgggaag ccaccgtgcc
     1921 gccgaagtgc tgggtacgcg cccagatccg gagcgcggct tcacgagagg ccctgcaaac
     1981 caccccgtgg cagggtccgg ccggcgaggg aagctggtac gagcgcggcg gggagatccc
     2041 gccccaccac cccgccggac ggtgggtgca ataccggctc gcgctgggcg cgcgcaacgg
     2101 ctgcggcacg cccagggtca ccacggtccg cctggtctgg cgcgacgggg cggagtgacc
     2161 cgccgcgccc cctgaatttg ccggctcctt atggcgaggg gatcgcgacg acctctcctt
     2221 tctggagcga ctcgagggcg gcgaagggga tcgccccgga catgtaggca tccaggatgc
     2281 cgcgctcggg gtcgtcgccc tgctccaagc gctccaggaa gtgcgagaat agcgcccggt
     2341 cgtgatgctg gtggcctgtg cctgtgggcc ggggcacctc gatcacatcc accgggcggt
     2401 ccggcagtgg ccagtactcg atgcgatact cctccatgtc gcaccgaatc tcgccccgcg
     2461 tgccgatgaa cgtcatgcgc cgccggccct ggggcgagac catcgacagc gtgtaggaaa
     2521 ggcgcattcc gttggtgaac tccgccgtga ggctgaccgt gtcggtgctg tccttctcat
     2581 tgttgtagac gcacatatcc tcggggagca cctgggggcc ggcggtgttg tagaagcgct
     2641 cgcgatactc cgggccctgg tcggtgtaat agatgcagtc gtcggtcagg cggcaggcgt
     2701 ggcaatggct cgccgcgcct tcccgaggct ggaacacctc ggtgcccccg aaagcggcca
     2761 cccgcgccgg atacgccccg gtaatgaagt tgatgacgtc gaaggtgtgg caccccttgt
     2821 ggaggagcat gtcgccgctg aacttccgga accggttcca gcggcgcatg taggtgctgc
     2881 cgtggaacca cccgatcgcg tcgtgcgtaa tgcaggcgat cacccggccg atggcaccgc
     2941 ccgcgaccaa ctcacgcagc ttgaggatgc acgggtcgta gcgaaagacg aagccgagat
     3001 gaagcagctt gccggaacgc ctggccgccc ggcagatcgc gcgcgcgtcc tccggggtcg
     3061 tggcgagggg cttctccacc aggcagtgga accccgcttc gaagcacgcc accgctggct
     3121 cgcggtggat gctatccggc gtgcagatca gaacggcgtc gatgtcattc cggcgcacca
     3181 gctcctcgac tgaaaggatg cgcggcgccg tcaggccgtt gcggtcgcag aagtcgttca
     3241 agacgccctc gttgcggtcg cacatcgcgg cccagaccac acgtccggcc ggcatgcgct
     3301 tgaccacgtc cacgtagatc tgcccgcgac cgcccgtacc caccaacgcc aatcgaatcg
     3361 gattcatctc gtcgtgatcc cttcgcatgg accgtggccg ggcacttcgc cctccaggtg
     3421 tgcggcgccc gcccggctct ctctcacaca cggggtgttc cgtcacccgc gcgcggagct
     3481 cgaagctccg cgctgcctcc aggcaaagtc cctctggggc ttcccctccc agcccgcgcc
     3541 gttctagccc gaagtattca ccagaatact tcaagagaag actcttgcct ggtagcacgt
     3601 cgttgccgcc acaagcgccc ggtggcagag tgttttcgag gcagtgacca cgaacccgcg
     3661 ccacgcttgg cagagagact tcttttcatt attctgatga at
//
LOCUS       DAOSCV010000411         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15843_length_3699_cov_4.895719, whole genome shotgun sequence.
ACCESSION   DAOSCV010000411 DAOSCV010000000
VERSION     DAOSCV010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15843_length_3699_cov_4.895719"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLY56_10665"
     CDS             complement(<1..499)
                     /locus_tag="PLY56_10665"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOJ21987.1"
                     /translation="MDWHDEIARALDQVREKRPLVHNITNYVVMNSTANALLALGASP
                     VMAHAVEEVEELVALSGALVLNIGTLSGPWIAGMFRAGRRACDLGIPIVLDPVGAGAT
                     RLRTDTSRRLLEELHPSVLRGNASEILALGAGEGGARGVDAAHTVDQARQTAIQLARA
                     HGIVVA"
     gene            820..1104
                     /locus_tag="PLY56_10670"
     CDS             820..1104
                     /locus_tag="PLY56_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ21988.1"
                     /translation="MKIGRQSRTGFTLIELLVVIAIIAILAAILFPVFARARENARKA
                     NCQSNLKQIGLAFQQYRQDYDETWPWGGSTPVISTSRSRAAVGRAGWATC"
     gene            1020..1586
                     /locus_tag="PLY56_10675"
     CDS             1020..1586
                     /locus_tag="PLY56_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21989.1"
                     /translation="MALGGIDTSDIDVALARSGWTGWVGNVLIPYVKNTGIFACPSDS
                     LNQLNVAKGVPNPIPAQYQGRIFKVSYCYNYSGVDYDPTATSLPPGCGRADAAMLRPA
                     ELAVMWDSQNRWADGQELWTRDIAQFNAGNLQYGCRHSGMANFLFADGHVKADRFDKM
                     KYRNFCNLPDGHAKLDQPVNNAVAWSYN"
     gene            complement(1641..2486)
                     /locus_tag="PLY56_10680"
     CDS             complement(1641..2486)
                     /locus_tag="PLY56_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ21990.1"
                     /translation="MKLDLTAAHLLMVTGGICAGAREGAGEIAPWPGEAGEYHGYERH
                     DFTLDGCACTVVLPTAALPGRPWIWRAEFFDAFPQIDLALLKEGFHLAYMNVGNTFGC
                     PSAMAHFDVFYDTLVNRHELMERVVLEGLSRGGLYVYHWGASHPDKVACILGDNPVCD
                     IKSWPAGKGRAPGSPNDWKKLIADYGFRDEAEALAYDRNPLDWAILKPLAERRVPLLH
                     ICGDADEAVPYEENTAILRERYEQLGGPITVIIKPGQKHHPHGLEDPAPAVAFIREAV
                     YGKPR"
     gene            complement(2563..>3699)
                     /locus_tag="PLY56_10685"
     CDS             complement(2563..>3699)
                     /locus_tag="PLY56_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21991.1"
                     /translation="HGNPPKCLVTAKGEILARDEKVPSTTAIHWDADALREIVAGRRI
                     LKYNGQVVFDGLEGHQAVWADVIGDWREEIITSVPGELRIYTTTIPATDRRVSLVRDP
                     IYRYDLAHLAMGYAQVPCTGYYIAQKGPAVWMSTSTTSIRAGEPVQVKAMLSAPAREA
                     VEGTLKLTVPEGLTASATEARVKAAPGEIAEATFTISLAQAPKLLSGAKVYPLTLTLE
                     GADRLSTVASLRAEEELLRDVPMVQAEDVAAQEGGEIQVRDDKPNVVGRSFSHWDNAG
                     HRLTWKIQVPAEGDYLLVVRYCTPVTVQREVQIDGGAPIKQSFVGTGGFSSTVSDWAH
                     GTVRASDRKPRVFRLKPGEHTITMTNLDGRGMNLDYLALVPAGK"
BASE COUNT          621 a         1190 c         1226 g          662 t
ORIGIN      
        1 ccgcgacgac gatgccatgc gctcgtgcca gttggatcgc cgtctgccgg gcctggtcca
       61 cagtgtgagc ggcatccacc ccgcgcgcgc ctccctcccc cgcgcccagg gcgagaattt
      121 ccgaggcgtt gccccgaagc acggagggat ggagctcttc cagcaggcgc cggctggtat
      181 cggtgcgcag gcgcgtggct ccggctccga cggggtcgag cacgatcggg atgcccagat
      241 cacaggcgcg ccgccccgcg cggaacatcc ccgcgatcca ggggcccgag agggtgccga
      301 tgttgaggac gagcgcgccg gagagcgcta ccagctcctc gacctcctcc acagcatgcg
      361 ccatcaccgg cgaggcgccc aaggctagca gcgcgtttgc cgtcgagttc atcacgacgt
      421 agttggtgat gttgtgcacc agggggcgct tctcgcgcac ctgatcgagt gccctggcaa
      481 tctcatcgtg ccaatccact tgcatcctcc tgctctctct gcaatacttc gtttggtcac
      541 aaaggtcaag cgatcgtacc atgcgggcga gcgtcctgtc aaccggtgtg gagggggtgg
      601 gaagcaagcc aacgggctgt tgacagacgt tcgcagaggt ggtagagtga taaacagtct
      661 agtaggtttc cattcacgtt cccttcggat cacgtctccc ggccttggca aagcacccct
      721 ggggaagggg agcgcgcgct tcctagccga aagagagatc cgaggggggt accggtgttc
      781 catggtggcg cgccgcgacg gaaggaggca gaagaaagaa tgaaaatagg cagacaatcg
      841 cgtacggggt ttacgctcat cgagttactc gttgtcattg ctatcattgc tatactggca
      901 gcaattctct tcccggtctt cgcacgggcc agagagaacg cgcgcaaggc aaactgccag
      961 agcaacttaa agcagattgg attggccttt cagcagtacc gccaggacta tgatgagacg
     1021 tggccctggg ggggatcgac accagtgata tcgacgtcgc gctcgcgcgc agcggttgga
     1081 cgggctgggt gggcaacgtg ttgataccct acgtcaagaa caccgggatc ttcgcgtgcc
     1141 ccagcgattc cttgaaccag ctgaacgtgg cgaaaggcgt gccgaatccc atcccggcgc
     1201 agtatcaggg gagaatcttc aaagtctcct actgctacaa ctactcgggc gtggattatg
     1261 atccgacggc cacgtcgctg ccgcccgggt gtggccgtgc cgatgccgct atgctgcgcc
     1321 ccgcggagct ggcggtgatg tgggacagcc agaaccgctg ggcagatggc caggagctgt
     1381 ggacgcgcga tatcgcccag ttcaacgcgg ggaacctcca gtacggctgc cggcatagcg
     1441 ggatggcgaa cttcctcttc gcggatgggc acgtgaaggc ggaccggttc gacaagatga
     1501 agtatcgcaa cttctgcaac ctgccggatg gccatgcgaa gctggaccag ccggtgaaca
     1561 acgccgtcgc ctggtcgtac aactgagcgc gcaggtggct tggcacggga cgcggcggag
     1621 cttgccgcgt cccgcgccat ttaacgcggt ttcccgtaga ccgcttcgcg gataaaggcg
     1681 acggccggcg cgggatcctc caggccgtgg ggatggtgct tctggccagg tttgatgatc
     1741 accgtgatcg gcccgcctag ctgctcgtag cgctcgcgca ggatcgcggt gttttcctcg
     1801 tagggcaccg cttcatccgc gtcgccgcag atgtgaagca gaggcacgcg ccgctcggcg
     1861 agaggcttca gaatcgccca gtccagcgga ttgcggtcgt aggccagcgc ttcggcctcg
     1921 tcgcgaaagc cgtagtccgc gatgagcttc ttccagtcgt tggggctgcc gggcgccctg
     1981 cctttgccgg ccggccagct cttgatgtcg cagacggggt tgtcgcccag gatgcacgcc
     2041 actttgtccg ggtggctggc accccaatga tagacgtaga gcccgccgcg gctgagcccc
     2101 tccaggacaa cccgctccat cagctcgtgc cggttcacca gggtgtcgta gaagacgtcg
     2161 aagtgcgcca tggcgcttgg acaaccgaag gtgtttccca cgttcatgta ggcgagatgg
     2221 aagccctcct tcaggagcgc caggtcgatc tgcgggaaag cgtcgaagaa ctccgcgcgc
     2281 cagatccagg gccgcccggg aagggccgcc gtgggaagga ccacggtgca ggcgcagcca
     2341 tccagcgtga aatcgtggcg ctcgtagccg tgatactccc ctgcttcccc gggccacggc
     2401 gcgatctccc cggctccctc gcgggcgccc gcgcagatcc cgcccgttac catcaggagg
     2461 tgcgcggcgg tcaggtccag cttcatatct cctcttttcg tcacaagcgg cgccctgctt
     2521 ctggaagcag ggcgccgctt ccggcgttca tggcgacgcc ggtcacttcc cggctggcac
     2581 cagcgccagg tagtcgaggt tcatcccccg gccatccagg ttcgtcatgg tgatcgtgtg
     2641 ctcgcctggc ttgaggcgga acactcgggg ttttcgatcc gacgcgcgca cggtgccgtg
     2701 cgcccagtca ctgacggtgc tgctgaagcc gcccgtgccc acgaagctct gcttgatcgg
     2761 ggcgccgccg tcaatctgca cctcgcgctg gactgtcacc ggagtgcagt agcggactac
     2821 cagcagatag tctccctcgg cgggcacctg gatcttccag gtgagccggt ggccggcgtt
     2881 gtcccagtgg gagaacgagc ggcctaccac gttgggcttg tcatcgcgca cctggatctc
     2941 gccgccctcc tgtgcggcca cgtcctcggc ctgcaccatc ggcacgtcgc ggagcagctc
     3001 ctcctcggcg cgcagcgagg caacggtgga gagccggtcg gcgccctcca gggtgagcgt
     3061 gagcgggtag accttggcgc cgctcagcag ctttggcgcc tgcgccagcg agatggtgaa
     3121 ggtggcctca gcgatttcgc ccggcgcggc cttcacgcgc gcctcagtcg cggaggcggt
     3181 gaggccctcc ggcacggtga gcttcagcgt tccttccacg gcctcgcgcg cgggcgcgga
     3241 gagcatcgcc ttcacctgca ccggctcgcc ggcgcggatg ctggtggtcg aggtgctcat
     3301 ccacacggct gggcccttct gggcgatgta atacccggtg cacggcacct gggcatatcc
     3361 catcgccagg tgggccaggt cgtagcggta gatcggatcc cggacgaggg agacgcgccg
     3421 gtccgttgcc gggatcgtgg tggtatagat gcgcagctcg cccggaacgg aggtgatgat
     3481 ctcctcgcgc cagtcgccga tcacatcagc ccacaccgcc tggtggccct ccaggccgtc
     3541 gaagacgacc tgaccattgt acttgagaat gcgccggccg gccacgatct cgcgcagggc
     3601 atccgcgtcc caatggatcg cagtggtgga tggcaccttc tcatcacgcg ccaggatctc
     3661 gcccttggcg gtgaccagac acttcggcgg gttgccgtg
//
LOCUS       DAOSCV010000412         3694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15878_length_3694_cov_4.047541, whole genome shotgun sequence.
ACCESSION   DAOSCV010000412 DAOSCV010000000
VERSION     DAOSCV010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15878_length_3694_cov_4.047541"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="PLY56_10690"
     CDS             <1..801
                     /locus_tag="PLY56_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21992.1"
                     /translation="VQYSHLICYVDNVPLVHHLSAAAILQRDPETRTLRVWRWSGVRR
                     HLLVPKAYVDYSLLESLGVPVENTMDESENAPREAVALRALAHSKSQRMRRQIEVELI
                     RQWVAAREEGWLMVDGPLMLLPELDVRGRMVGVIKSFGAQFFDGEEQLRVMTLPKWHR
                     TTAFQLPRGSRADAGAATARHWYSWYVRIRDPRGSDTDHGLLRVEIPPDPEILAQADT
                     ISRWLIAERAPLSTPDPRWDNLLYPIHACEQFLRSRLPERRQVHLYLK"
     gene            complement(815..1837)
                     /locus_tag="PLY56_10695"
     CDS             complement(815..1837)
                     /locus_tag="PLY56_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOJ21993.1"
                     /translation="MYHRLVAIVWLVLAWCLPVTAAEPGSSKPEPNAGRIVVAVAGFV
                     GDDTRLRNAITQTFTVSLAQSKRLRLVERERLAKILEEQKLENSGLVDPEQAARIGKL
                     LGASKLVAGGVTRLGSLVSIDARLIDVETGKVEDGGAEQVFGRIQGEGEEVHALARQL
                     ATRFHRAITGEWLPHALLSDRAGLSPGAEIDPLALLERGDGGIRLEVKLDRDEGSTYR
                     WGETMRISFRVDTPCHVYLFNVDTARQVTQLYPNEWHRDERVEANRWYQVPGPGEEWE
                     LVIEGEMGLEEIIAIASTRPLGTPASIATVSKSAAQFAGKTVMPRLRQAPAAWAGARI
                     RFFTTK"
     gene            complement(1914..2690)
                     /locus_tag="PLY56_10700"
     CDS             complement(1914..2690)
                     /locus_tag="PLY56_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ21994.1"
                     /translation="MRIGCCAYSYRDALTSGSMTLEQFLDAAVAMNLDGVELTTYYLK
                     QADRDYLNRIKRECFRRGLHISGTAVGSNFCQAEPAKRREHVQMTKQWIDCSVLLGAP
                     CIRVFAGPVPPGHTEEDAVAWAVECLQECAEHGAARGVVVALENHGGITARAEQVVRL
                     VKLVDRPWFGINLDFGNFREDPYREFEMVAPYMVSAHAKTHWRGPSGEEPVDYLRALQ
                     IARDAGYRGYVNIEYEGRSDPATAVPRFAAELKEIVRRLA"
     gene            complement(2849..>3694)
                     /gene="hisD"
                     /locus_tag="PLY56_10705"
     CDS             complement(2849..>3694)
                     /gene="hisD"
                     /locus_tag="PLY56_10705"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ21995.1"
                     /translation="AGVPFIELCTPPGPDGRVDPHILYAASACGIETAFKVGGAQAIA
                     AMAYGTATVPRVDKIAGPGNIYVLLAKREVFGDVGIESLPGPSEIAVLADSTARPAYV
                     AADLLSQAEHGGDNLSVLVTPSEDLARAVIAEVERQTERSPRAEIIRQSLAFGGACVV
                     VSSLDEGIEIVNAFAPEHLEIVTAEPMALLGRIKAAGAILLGEASSEPVGDYIAGPSH
                     VLPTGGTARFSSPLSVDDFLVKSSVIAYTPERLRADAEHILALAEVEGLDAHANAVRV
                     RLANE"
BASE COUNT          595 a         1300 c         1212 g          587 t
ORIGIN      
        1 gtgcagtata gccacctgat ctgctacgtc gacaacgtgc ccctggttca ccacctcagc
       61 gccgccgcca tcctccagcg cgaccccgaa acccgaacgc tccgcgtctg gcgctggagc
      121 ggcgtccgcc ggcacctgct cgttcctaag gcgtacgtgg attacagcct cctcgaaagc
      181 cttggcgtgc ccgtcgagaa cacgatggat gagagcgaga acgccccgcg cgaagccgtt
      241 gccctgcgcg cgctggcgca cagcaagtcg cagcggatgc gccggcagat cgaggtggag
      301 ctgatccggc agtgggttgc cgcccgcgaa gagggctggc tgatggtcga cggccccttg
      361 atgctgcttc cggagctcga cgtgcgcggg cggatggtgg gggtgatcaa gtcgttcggg
      421 gcgcagttct tcgatggcga ggagcagctt cgcgtgatga ccctcccgaa gtggcaccgg
      481 accacggcat tccagctgcc ccggggtagc cgggcggatg ccggagcggc gaccgcgcgc
      541 cactggtact cctggtacgt ccggattcgc gaccctcgcg gaagcgacac ggatcatggc
      601 ctcctccgcg tggagatccc acccgatccg gagatcctgg cgcaagccga cacaatctcg
      661 cgctggctca tcgccgagcg tgcgcccctc tccacgccgg acccgcgctg ggataacttg
      721 ctctacccca tccacgcctg cgagcagttc ctccgaagca ggctccccga gcgccgccag
      781 gtccacctct acctgaaata gaccacccgg aggctcactt cgtggtgaag aaccggatcc
      841 gcgcgcctgc ccaggcagct ggggcctggc gcagcctcgg catcaccgtc ttaccggcga
      901 actgcgcggc gctcttgctc actgtcgcaa tcgacgccgg ggtgcccagc ggccgggtcg
      961 aagcgatcgc gatgatctcc tcgagcccca tctcaccctc gatcaccagc tcccactcct
     1021 caccgggtcc gggcacctgg taccagcggt tggcctccac cctctcgtcg cgatgccact
     1081 cgttgggata gagctgcgtc acctggcgcg cggtgtccac attgaagagg tagacatggc
     1141 agggggtatc gacgcggaag gagatccgca tcgtctcgcc ccagcgatag gtgctcccct
     1201 cgtcgcggtc gagctttacc tccaggcgga tcccgccgtc tccgcgctcc aggagagcga
     1261 ggggatcgat ctcagcgccg ggagagaggc cagcacggtc ggaaagcaag gcgtgcggca
     1321 gccactcgcc cgtgatcgcg cgatggaacc gcgtcgccag ctggcgtgcg agggcgtgga
     1381 cttcctctcc ctcgccctgt attcgcccga acacctgctc ggcgccgccg tcctccacct
     1441 tgccggtctc aacatcgatc agccgggcat caatcgagac aaggctcccc aggcgggtga
     1501 cgccgccagc gaccagcttg ctcgctccca ggagcttccc gatccgcgcc gcttgctccg
     1561 ggtcgaccag gccgctgttc tccagcttct gctcctccag aatcttggcc aggcgctcgc
     1621 gctcgaccag ccggaggcgt ttcgactggg cgagcgagac ggtgaacgtc tgggtgatcg
     1681 cattgcggag gcgcgtgtcg tcgccgacga acccggcgac ggctaccacg atccggcccg
     1741 cgttcggctc cggcttgcta gatccgggtt cagcggcagt caccggcagg caccatgcga
     1801 gaaccagcca gacgatggcg acgagacgat ggtacatggg gaagcaactc ctacccgcag
     1861 gcagcgtgat ccgaccgcta tcttcgatga cgcgcagctg gagagcggca aagtcaagcc
     1921 aggcggcgca cgatctcttt cagctctgcc gcaaagcggg gcaccgccgt ggctggatcg
     1981 gaccggcctt catactcgat gttgacgtag ccgcggtagc ccgcgtcgcg cgcgatttgg
     2041 agcgcgcgaa ggtagtccac cggctcctcg ccgctcgggc cgcgccagtg cgtcttggca
     2101 tgcgccgaga ccatgtaggg cgccaccatc tcaaactcgc ggtaggggtc ttcgcggaag
     2161 ttgccgaagt cgaggttgat cccgaaccag gggcggtcca ccagcttcac caggcgcacc
     2221 acctgttccg cccgcgccgt gatccctccg tggttttcca gggccaccac cactccgcgc
     2281 gcggcgccat gctcggcgca ctcctggaga cactccaccg cccacgcgac ggcgtcctcc
     2341 tccgtgtgcc cgggcggcac gggcccggca aagacgcgga tgcacggcgc tcccaggagc
     2401 acggagcagt cgatccactg cttcgtcatc tgaacatgct cgcggcgctt cgcgggctcg
     2461 gcctggcaga agttgctgcc cacggcggtg ccggagatgt gcagccccct gcggaagcac
     2521 tcgcgcttga tgcggttgag gtagtcacgg tcggcctgct tcaggtagta ggtggtcagc
     2581 tccaccccgt cgagattcat cgcgaccgcg gcatccagga actgttccag ggtcatggag
     2641 ccgctggtga gcgcgtcgcg gtacgagtag gcgcagcacc cgatgcgcat gggtgtctcc
     2701 ttccctccgt gagccagaag cagcctgcgg aaagcacaga gccatcaggg gcgcgtgcca
     2761 tacggccgtg cctactcctc cgttcggcgc cctgcccgcc ttctccttta ccaagcagag
     2821 agcccggctc cccctggtcg gaagagggtc actcgttcgc caggcggacg cggacggcgt
     2881 tggcgtgcgc gtccaggccc tccacctcgg ccagggccag gatgtgctcc gcgtcggcgc
     2941 gcaggcgctc cggcgtgtag gcgatgacac tggatttcac caggaagtcg tccacgctga
     3001 gtggcgagga gaagcgcgcc gtgccccccg tggggagaac gtggctcggg ccggcgatgt
     3061 aatcgcccac cggctcggac gaagcctcgc ccagaagaat ggcgccggcc gccttgatcc
     3121 ggccaagaag cgccatcggc tccgccgtga cgatctcgag gtgctccggt gcgaaggcgt
     3181 tgacgatctc gatcccctcg tccagcgacg agacgacgac gcacgcgccc ccgaacgcga
     3241 gcgattggcg gatgatctcc gcgcggggcg agcgctccgt ctgccgctcc acctccgcga
     3301 tcaccgcgcg agcgagatcc tcggaaggcg tcaccagtac cgagaggtta tcgccgccgt
     3361 gctcggcctg agagaggagg tccgccgcca cgtaagccgg gcgcgccgtg gagtccgcca
     3421 ggacggcgat ctcggagggg ccgggcaacg attcgatccc gacgtcgccg aacacctcgc
     3481 gcttggccag gaggacatag atgttgcccg ggccggcgat tttgtcgacc cggggcacgg
     3541 ttgccgtgcc atacgccatg gcagcaatcg cctgggcgcc acccaccttg aatgcggtct
     3601 cgatcccgca cgcgctggcg gcgtagagga tgtgcgggtc gacgcggcca tcgggccccg
     3661 ggggcgtgca cagctcgatg aacggcactc ccgc
//
LOCUS       DAOSCV010000413         3682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15947_length_3682_cov_3.618969, whole genome shotgun sequence.
ACCESSION   DAOSCV010000413 DAOSCV010000000
VERSION     DAOSCV010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15947_length_3682_cov_3.618969"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLY56_10710"
     CDS             complement(<1..197)
                     /locus_tag="PLY56_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ21996.1"
                     /translation="MKPRGVGPTALLLAALAALAPSLKAAAAPSGLGLIPTADLLPPG
                     VASLEVETVGDHVLPGDQGET"
     gene            complement(597..2447)
                     /gene="feoB"
                     /locus_tag="PLY56_10715"
     CDS             complement(597..2447)
                     /gene="feoB"
                     /locus_tag="PLY56_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ21997.1"
                     /translation="MHTHDTPLADPSVRRECRSLVLAGNPNTGKSVFFNFLTGIYVDV
                     SNYPGTTIEVSRGRCGEMDVIDTPGVYGVSSFNDEERVARDIILEADVVLNVVDAVHL
                     ERDLFLTLQLIDMGIPMVVALNLMDEAKREGVSIDVDLLSDLLGVPVIPTVATRGEGL
                     CQVQEAIASARPGHSDPQLLDRVHEVLREVGSRPEAVLVLEGDPVVSERHGVAPRELR
                     EEIYARRRMRVNDIVRHVVREESPRFSLSAALGRWTLHPVTGLVTLAAVLWVMYQFLG
                     VFIAQTVVGYTEGELMQGYWEPWARATAARWFGDGSLAHTLLVGEFGVVTMTVTYLLG
                     LLLPLVAGFYFSLSILEDSGYLPRLATLVDRFLTGLGLNGRAVVPIILGFGCITMATI
                     TTRLLGTVREKRIATAILNFTIPCSAQLGVITALLARAGGEMALAYVVVIGSCLVLVG
                     TLLNRALPGESSPLLIDLPPMRLPRIENVLRKTVTRTYHFLREASGWFVLGSFVVVVM
                     SVTGLLELWQSALSPMVVSWLQLPREAANAFVMGMVRRDFGAAGLAMMPLTAKQTLVA
                     LVTMTLFVPCIASVAILLKERGRREALIIWGASWILAFAVGGLVSQIVIR"
     gene            complement(2453..2677)
                     /locus_tag="PLY56_10720"
     CDS             complement(2453..2677)
                     /locus_tag="PLY56_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOJ21998.1"
                     /translation="MTLDQVRKGSVVQILSIPGAEERAQLIRLGLGEGCRVRCAERLP
                     LGPLVLRCHRQEVALGRNLARRIEVREESE"
     gene            complement(2952..>3682)
                     /locus_tag="PLY56_10725"
     CDS             complement(2952..>3682)
                     /locus_tag="PLY56_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ21999.1"
                     /translation="ATYTGVFDMIRDLFSHTPEARARGYMPGRFSFNVKGGRCEACKG
                     DGIIKIEMHFLPDIYVPCEVCKGKRYNRETLEVRYKGKTIADVLNMTVDEALAFFESI
                     PPIQRKLSTLHDVGLDYIKLGQPATTLSGGEAQRVKLATELSRRSTGRTLYILDEPTT
                     GLHFADIEKLLEVLGRLVDSGNSVVVIEHNLDVIKVADWVIDMGPEGGEGGGRVVAQG
                     PPEVIAACPESHTGRFLKNILERG"
BASE COUNT          725 a         1207 c         1154 g          596 t
ORIGIN      
        1 aacgtctccc cctggtcgcc gggcagcacg tgatcgccca cggtctccac ctcgaggctg
       61 gccacaccgg ggggaagcag atccgcggtc gggatcaagc cgaggccgga gggcgcagcc
      121 gctgccttta gcgagggggc cagggccgcg agcgcggcca gaaggagcgc ggtagggccg
      181 acaccgcgag gcttcatccg tgacagcctc tccatcatgc gagcccatgc gcctggcgag
      241 gaggcgccgc ccgcggcgca ctttcgacac gaggcgcagc cctcgaacgg atggccgcag
      301 cgggggcaac gccatccggg aagtcgatcg taaatcgcgc cgcacccgga acaggtgagc
      361 gagcaggagg atgtgccttc ccctggcgtc tctgccttgc gcgcctcgcg ggcaaccggc
      421 tcctgtgtcg cgcttcgtgc caccgacaga ctcccttctt gattcgcaaa gctccgtaca
      481 gccgccacgg cacagccacc acggccgtgg cggacagaat aatcgcgggg tagaaagcag
      541 gcgcgtcgcg gggtatcggg tcgttttgcg cgcctgcgcg ttcagcatcg tatttctcag
      601 cggatgacga tctgcgatac gagaccgccc actgcaaagg cgaggatcca ggatgccccc
      661 cagatgatca gcgcttcgcg ccgcccccgt tccttgagga ggattgcaac ggaggcgatg
      721 catggcacga agagcgtcat cgtcaccaga gcgacgagcg tctgcttggc agtcaggggc
      781 atcatcgcca ggccagccgc gccaaagtca cggcgtacca tccccatcac gaaggcgttg
      841 gcggcttcgc gcgggagctg cagccagctg acgaccatgg gcgacagtgc gctctgccac
      901 aactccagca agccggtcac actcatcacc acgaccacga aggagcccaa gacaaaccat
      961 ccggacgcct cgcggaggaa gtggtaggtc cgcgtgacgg tcttgcgcag cacattttcg
     1021 atacgaggca ggcgcatcgg tggaagatcg atcaggagcg gcgacgactc cccggggagt
     1081 gcccggttca acagggtgcc cacaaggacc aggcagctac cgatcaccac cacgtaggcg
     1141 agcgccatct ctcccccggc acgggccagg agcgccgtga tcacgccgag ctgcgccgag
     1201 caaggaatgg taaagttgag gatcgccgtg gcgatccgct tctcgcgaac cgtccccaga
     1261 agccgggttg tgatcgtcgc catggtgatg catccgaagc ccagaatgat cggtaccacg
     1321 gcgcggccat tcaggccgag gccagtcagg aagcgatcca ccagagtcgc cagccgcggg
     1381 agatagccgg agtcctccag aatcgacagt gagaagtaga atccggcaac aagcggcagc
     1441 agcagcccga ggaggtaggt gacggtcatc gtgaccacgc cgaactcccc gaccaggagg
     1501 gtgtgagcca acgaaccgtc gccaaaccag cgggcggcgg tagcgcgcgc ccacggctcc
     1561 caatacccct gcatcagctc cccctcggtg tagccgacga ccgtttgcgc gataaaaacc
     1621 ccgaggaact gatacatcac ccagaggaca gcagcgagcg tcaccagccc ggtgacgggg
     1681 tggagggtcc accgtcccaa agcagccgag agggagaaac gagggctctc ttctcgaacg
     1741 acgtggcgga cgatatcgtt gacgcgcatc cggcggcgcg cgtagatctc ctcgcgcagt
     1801 tcgcgcggcg ccacgccatg ccgctccgat acgaccggat cgccttccag gacgagcacc
     1861 gcctccgggc gcgatcccac ttcgcggagc acttcatgga cgcggtcgag cagttgtgga
     1921 tccgagtgcc ccgggcgcgc cgaggcaatc gcttcctgca cctggcatag gccctcgcca
     1981 cgcgtggcaa ccgtcggaat gaccggcacc cccagcaaat ccgagaggag gtccacgtca
     2041 atgctcacgc cctcacgctt cgcctcatcc atcaggttca gcgccaccac catcggaatg
     2101 cccatgtcga tgagctgcag ggtgaggaag aggtcacgct ccaggtgaac cgcgtccacc
     2161 acgttgagca caacgtcggc ttccagaatg atatcgcgcg cgacgcgctc ctcatcattg
     2221 aaggacgaga caccgtagac tcccggggtg tcgatcacat ccatctcgcc gcagcggccc
     2281 cgcgatacct caatcgtggt gcccgggtag ttggagacgt cgacatagat gccggtgagg
     2341 aagttgaaga agacagactt gccggtgttt gggttgcccg ccaggaccag cgaccgacat
     2401 tcgcgccgca cggacggatc agcgaggggc gtgtcgtgcg tgtgcatcgt ggttattccg
     2461 attcctcccg gacctcaatg cggcgggcca ggttccggcc cagcgccact tcctgccggt
     2521 ggcagcgaag caccaacgga cccagaggca gccgctccgc gcagcgcacg cggcatccct
     2581 ctcctaagcc caggcgaatg agctgtgctc gttcctctgc gcccgggatc gacaggatct
     2641 gtaccacgct gcctttgcgc acctgatcca acgtcatcgt caccctccac aagaaaccgt
     2701 ttagatgtgc ctacgtgctt gttttagcct cagccaaatt atagggcccc ctgtgacgcg
     2761 cgtccataga cactttatct cttttttgga ttcattatca ctccacgcac gcctgttcat
     2821 caggcgctcg ttgtagaggg gcgtgtggat acgaaaggac cgaggagaga cgcgcaatga
     2881 ggggagatgg agcgaggaga gcccgcccct ccaccgcaca cggctcagtg gatgggcgga
     2941 aggtcagatg cctaccctcg ctccaggata ttctttagga aacggccggt gtgcgattcg
     3001 gggcaggcgg caatcacctc cggcgggcct tgagcaacga cgcgaccgcc gccctctcct
     3061 ccttcggggc ccatatcgat cacccagtcc gcaaccttga tcacgtccag gttgtgctcg
     3121 atgaccacca cgctgttgcc ggaatcgacc aggcggccaa ggacttccag gagcttctcg
     3181 atatcggcga agtgaagccc ggtggtcggc tcgtccagga tgtagagcgt gcgcccggtg
     3241 ctacgacgag agagctccgt ggcgagctta acgcgctgcg cttcgccacc ggagagcgtg
     3301 gtcgcgggct gccccagctt gatgtagtcc aggcccacat cgtggagggt ggagagcttg
     3361 cgctggatcg gcggaatgct ttcgaagaac gccagcgcct cgtcaacggt catattgagg
     3421 acatcggcga ttgtcttccc cttatagcgc acttcgagcg tctcgcggtt ataccgcttt
     3481 cccttgcaca cctcgcacgg cacatagata tcgggcagga agtgcatctc gatcttgatg
     3541 atgccatccc ccttgcacgc ctcacatcgc ccgcccttca cgttgaagga gaagcggccg
     3601 ggcatgtagc cgcgggcgcg tgcctcgggc gtgtgcgaga agaggtcgcg gatcatgtcg
     3661 aacacgcccg tgtaggtcgc cg
//
LOCUS       DAOSCV010000414         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15977_length_3675_cov_3.669613, whole genome shotgun sequence.
ACCESSION   DAOSCV010000414 DAOSCV010000000
VERSION     DAOSCV010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15977_length_3675_cov_3.669613"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1298)
                     /locus_tag="PLY56_10730"
     CDS             complement(117..1298)
                     /locus_tag="PLY56_10730"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ22000.1"
                     /translation="MDQLRIGSLARPLEPLAPEDTLARATEVIRSVPFDRVPVAVEGR
                     LVGVVTAATLGSAAQTVLQDGISPEEIRLLSIDESVLSTSPALPADASLEEALSLLRH
                     HGGSLPVIRADGLYLGMVDQADVAGALLQKARPPSIGGMATPLGVYLTTGVFSAGARG
                     SLGLALTGAAIGSATVVVSVLMGLLIYGLERTTHFPIRASLNSVPAQWLDLNPYDIWG
                     HILPIIALALFALLFRLLPLTGCHAAEHQVVHAIERDCPLTPESVARMPRVHPRCGTR
                     FFAVGVLLVLFGNGLPISVWWIRYTAAAVAIVLWWRVLGDELQNYVTTKPASPKQIRS
                     AIAAAEELLAKYAHSPGVRASLPTRIWHMGILQVAAGVYFSVTLLTFLAGHYGVPLFM
                     P"
     gene            1517..1732
                     /gene="rpmB"
                     /locus_tag="PLY56_10735"
     CDS             1517..1732
                     /gene="rpmB"
                     /locus_tag="PLY56_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOJ22001.1"
                     /translation="MAKVCEFCGKKPMYGQNIRHVHSGGWARRAPRTKRRFLPNLQSV
                     HALVNGTQKKVRVCTKCLKKPGLVVRG"
     gene            complement(1854..2474)
                     /locus_tag="PLY56_10740"
     CDS             complement(1854..2474)
                     /locus_tag="PLY56_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22002.1"
                     /translation="MPMEEHQERPPRPADSRLLQSARLAGPFYGPRVSLTTPTPECCH
                     RLALIQRGDTLLGRQLEQTPDLPRRVATAAGAEFLHNLRAWCEERNGVAYAIAGPDGE
                     AIGLITLYDVDINQGAGRVGFWLASSAWGRGYAAEAFALLARYAAGRGLRQFHARIPR
                     QQTATWRVWSRLGARIEEDTETGFFRCTLDSSAHGFDAALEHALRE"
     gene            complement(2606..3067)
                     /locus_tag="PLY56_10745"
     CDS             complement(2606..3067)
                     /locus_tag="PLY56_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ22003.1"
                     /translation="MRIGSALPPLDGATEWVNSGPVGSADLAGRPLLVHFWALSCHSC
                     HEMMPTVVAWHQRYASRGLQVISVHQARCEADTDVAAIKQAIEKCGITHPVAIDNDFR
                     IVDAWENKFVPSFYLFNADGKLVHYQAGDRGQKMIEQAIERVLGAAASANA"
     gene            complement(3072..3617)
                     /locus_tag="PLY56_10750"
     CDS             complement(3072..3617)
                     /locus_tag="PLY56_10750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOJ22004.1"
                     /translation="MPLVGSKAPEFELPVFDPNDPTNTAKTVKLSDYRGKWLVFFWYP
                     LDFTFVCPTEITALSDRLDEFQEIGAEVLGASTDSIYSHKAWANTPRDQNGIAGLRYP
                     IAADMTHSVARDYGVLLEDQGVALRGLFIIDPEGVLQFATIHSLNVGRSVDEVLRVLQ
                     ALQTGGLCPADWRPGQKTLGG"
BASE COUNT          675 a         1149 c         1199 g          652 t
ORIGIN      
        1 cgccgcgcgc cgggttgcgc aatgcctccg aggcttcgga ttccttatct cggaatccca
       61 ggatccccgc tcacggcgcg acgattcgcc ttgccacgcg atagcaacga tgccgatcag
      121 ggcatgaata ggggcactcc ataatggccg gccaggaagg tgaggagcgt gacggagaag
      181 tagacccctg cggcaacctg caagatcccc atatgccaga tacgcgtggg aagcgacgcc
      241 cggactcccg gtgagtgtgc atacttggcg agtagctcct ctgcggcggc aatcgcgcta
      301 cgaatctgct tcggcgacgc gggttttgtg gtgacgtagt tctgaagctc atcgcccagt
      361 acccgccacc aaagcacaat cgccaccgcg gcggcggtat accgtatcca ccacaccgat
      421 atgggaagcc cattcccaaa cagaaccagg agcacgccca cggcgaagaa gcgcgtacca
      481 caccggggat ggactcgggg catgcgcgcc acgctctccg gggtgagcgg gcagtcccgc
      541 tcgatggcgt ggacaacctg gtgctcggcg gcatggcagc cggtcagtgg cagcagacga
      601 aacaggagcg cgaataaggc cagcgcgata atggggagaa tgtggcccca gatgtcgtac
      661 ggattcaggt ccagccactg cgcgggcacc gagttgagac tggcacgaat tggaaagtgg
      721 gtcgtacgct ccaggccata gatcaagagc cccatcaaga cgctgactac gacggttgcc
      781 gatccgatgg ccgcgccggt gagtgccagg ccaaggctgc cgcgcgcgcc ggcgctgaag
      841 acgcccgtgg tcaggtagac cccgaggggc gttgccattc ccccaatcga gggggggcgt
      901 gccttctgga gcagggcccc cgccacgtct gcctgatcca ccatgccgag gtagagacca
      961 tcggcgcgga tgaccggcaa cgaaccgccg tgatggcgta gcagcgagag ggcctcttcc
     1021 agcgaggcat cggctgggag cgcgggggat gtggagagca ccgattcgtc gatggagaga
     1081 aggcggatct cctcgggcga gatgccgtcc tgcaagacgg tctgggcggc gctgccaaga
     1141 gtggccgcgg tgaccacccc aaccaggcga ccttccactg ccacgggaac ccggtcgaat
     1201 ggcacggacc ggatcacctc ggtagcccgg gcaagggtat cctcgggagc gagcggctcc
     1261 agcgggcgcg ccagactccc gatgcgcaat tgatccatcg cttgcaatct ctccgcgtat
     1321 atgttatcat aatccggcct gtgaaggcaa tgaaatgggg ttgcatccga agccgcaagc
     1381 ggattaccgt gagccttggc ttaaggggcg agcccgccca ggcctgccgg tcgggttgtt
     1441 ccctgcaatt gcttgcggct ggtttggtcc ctggggcgcg tgctttgagc attggcaagc
     1501 ctgaaaggat acggatatgg ctaaggtctg tgagttttgt gggaagaagc ccatgtatgg
     1561 gcagaatatt cgccacgtgc actccggggg atgggcgcgc cgcgcaccgc gtaccaagcg
     1621 ccgcttcctg ccgaacctcc agagcgtgca cgccttggtg aatggaacgc agaagaaggt
     1681 tcgcgtctgc accaagtgcc tgaagaagcc cggcctggtt gtgcgcggtt gatcccgctc
     1741 accagactcg gtacagtgtt tcggccccca aagcctccgc gggaggtttc gggggccgaa
     1801 acggtgaatc aggctccctc cagtgacgac gccggccggt ggtggtgcgc tccctattcg
     1861 cgcaaggcat gctccagggc ggcatcgaag ccgtgggcgc tgctatccag cgtgcaacgg
     1921 aagaagcccg tctcggtatc ctcttcgatg cgggcgccca gacgtgacca gacgcgccag
     1981 gtggcggtct gttggcgtgg gatgcgcgcg tggaactggc gcaatccccg tcctgcggca
     2041 taacgcgcca ggagcgcgaa tgcctcggcg gcatagcctc tgccccacgc gctgctagct
     2101 agccagaagc caactcggcc ggcgccctgg ttgatatcga catcgtaaag cgtaatcaac
     2161 ccgattgcct cgccatccgg accggcgatc gcgtacgcga cgccgttgcg ctcctcgcac
     2221 catgctctca ggttgtgcag aaactcggcg ccggcggctg ttgctacgcg acgaggcagg
     2281 tccggggttt gctcgagctg tcttccaagg agcgtatcgc ccctttgaat gagggcgagt
     2341 cggtggcaac actctggcgt tggtgtggtg aggctcacac gcgggccata gaaggggcct
     2401 gccagtcgcg cgctttgaag caggcggcta tcggcgggcc gtggcgggcg ctcctggtgt
     2461 tcctccattg gcattgcctg atccccccgg tgcgcgcggg gtcgcactct ccccaattat
     2521 tgcccaatgg cggggtcggc aaaccagtca atgcagggtg ccgcgtgcca atccggcacg
     2581 cggctccccg gtcagatatg ttagatcatg cgttcgcgga agcggcggcc cctagaacac
     2641 gctcgatcgc ctgttcgatc attttctgcc cgcggtcgcc cgcctggtag tggaccaact
     2701 tgccgtccgc gttgaagagg tagaacgacg ggacgaactt gttctcccag gcatcgacga
     2761 tgcggaagtc gttgtcgatc gctaccggat gggtgatccc gcacttctcg atagcctgct
     2821 ttatcgcggc aacgtctgta tccgcctcgc agcgtgcctg gtggacgctg atcacctgga
     2881 ggccgcgcga cgcgtaccgt tggtgccagg cgaccacagt cggcatcatc tcgtggcagc
     2941 tatggcaact cagcgcccag aagtggacca ggagcggtcg tccggcgaga tcggccgacc
     3001 ccaccgggcc gctgttcacc cattcggttg ccccgtccaa cgggggcaac gccgatccga
     3061 tacgcatgcg cttatccccc caatgtcttc tggcctgggc gccaatccgc ggggcagagc
     3121 ccgcctgtct gcagtgcctg gagcacccgc aggacctcat ccacgctgcg gcccacgttg
     3181 agcgagtgga tggtggcgaa ctggagcacg ccttccgggt cgatgatgaa aagcccgcgg
     3241 agggccactc cctgatcctc gagaagcacg ccatagtcgc gcgcgaccga gtgagtcata
     3301 tccgcggcga tggggtagcg gagaccggcg atcccgttct ggtcgcgggg cgtgtttgcc
     3361 caggccttat ggctgtagat gctgtcggtg ctggcgccca acacctctgc accgatctcc
     3421 tggaactcgt ccagccggtc gctcaacgcg gtgatctccg tcggacagac gaaagtgaaa
     3481 tccaacgggt accagaagaa gaccaaccac tttccccgat agtccgagag cttgaccgtt
     3541 tttgccgtgt tggtcgggtc gttaggatca aacaccggca gctcgaattc tggagctttc
     3601 gatcctacga gcggcatgtt tcaactcctc tcaaagcgcg cggtcgtgtg gcggacttct
     3661 agctccggca cacgc
//
LOCUS       DAOSCV010000415         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_15991_length_3673_cov_4.056385, whole genome shotgun sequence.
ACCESSION   DAOSCV010000415 DAOSCV010000000
VERSION     DAOSCV010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15991_length_3673_cov_4.056385"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="PLY56_10755"
     CDS             <1..1568
                     /locus_tag="PLY56_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOJ22005.1"
                     /translation="LQDVGPTNRTGTTIRFKPDYQIFTKIEFRADMLTTRLRELAFLN
                     KGLKITFTDERTGQHEEFLYKGGIIEFVEHLNRNKEPVVPQKPVYFMRTREDVEVEIA
                     LQYNSTYQENILSYANNINTTEGGTHLSGFKTAITRVLNQYARKAGMLKDKDPNLSGD
                     DVREGLTAVVSVKVTQPQFESQTKIKLNNPEVEGIVNSVVGEGLSIYLEEHPSEAKRI
                     IDKALTAFRAREAARKASEMIKRQNYLESSTLPGKLADCTERDPSKCELFIVEGDSAG
                     GSAKMGRDRRYQAILPLRGKVLNVEKTRMDRALDNDAIAALITSIGTGISKSVHLHGD
                     QEENGDEENGNGHSNGRGNGNGNGNGNGKGHDVSRFDISKLRYHRIILMTDADVDGAH
                     IRTLLLTFLFRYMGPLVEQGHVYIAQPPLYQIRIGKKEDYAYSDEELQQKLKATDSKN
                     VSIQRYKGLGEMNADQLRDTTMDPATRTILQVRLEDAVKADEIFTILMGDKVGPRKEF
                     LDAHAKDVKELDI"
     gene            complement(1696..1872)
                     /locus_tag="PLY56_10760"
     CDS             complement(1696..1872)
                     /locus_tag="PLY56_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HOJ22006.1"
                     /translation="MAKQEEGGMTVREAGRKGGETTAERYGHEFYEEIGKKGGERGGP
                     RVRELIEKGKRQEK"
     gene            complement(2077..2247)
                     /locus_tag="PLY56_10765"
     CDS             complement(2077..2247)
                     /locus_tag="PLY56_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HOJ22007.1"
                     /translation="MSRTEGEMTVREAGRIGGNIRKKALGRRGYEELGHKGGQRVREL
                     IRKGKEAEQKGG"
     gene            complement(2399..2590)
                     /locus_tag="PLY56_10770"
     CDS             complement(2399..2590)
                     /locus_tag="PLY56_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012702924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Em GEA1 (EM1)"
                     /protein_id="HOJ22008.1"
                     /translation="MARRDEGDMTVREAGRKGGEATARTHGHEFYEEIGHKGGEIGGP
                     RGGQRVRELIEKGKELEEQ"
     gene            complement(2844..3059)
                     /locus_tag="PLY56_10775"
     CDS             complement(2844..3059)
                     /locus_tag="PLY56_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22009.1"
                     /translation="MPEGGMTVREAGRKGGETTARTHGHEFYEEIGKKGGNARKRELG
                     HEGYEELGQKGGQRVRELINKGKMQEK"
     gene            complement(3338..>3673)
                     /locus_tag="PLY56_10780"
     CDS             complement(3338..>3673)
                     /locus_tag="PLY56_10780"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HOJ22010.1"
                     /translation="RVFVVGGEVLGAVMRIAQGTEWRTNVHQGGVCVPYELSAYEREL
                     CLRAASVIGLEYTGIDLLPGPDGPVLLEVNGAPQWHGLAAATGIDVAGAIVEHALALA
                     RRRHPRASS"
BASE COUNT          682 a         1286 c         1082 g          623 t
ORIGIN      
        1 cactgcagga tgtcggcccc accaaccgaa ccggcacgac catccgcttc aagcccgact
       61 atcagatctt cacgaagatt gagttccgcg cagatatgct gaccacccgc ctgcgcgagc
      121 tcgcgttcct gaacaaggga ctgaagatca ccttcaccga cgagcgcacc gggcaacacg
      181 aggagttcct ctacaagggc gggatcatcg agtttgtgga gcacctgaac cgcaataagg
      241 agccggtggt gccccagaag ccggtctact tcatgcgcac ccgcgaggat gtggaagtcg
      301 agatcgcgct ccagtacaac agcacctatc aggagaacat cctctcgtac gccaacaaca
      361 tcaacaccac ggagggcggc acgcacctct ccggattcaa gacggccatc acgcgcgtcc
      421 tcaaccaata tgcgcgcaag gcggggatgc tgaaggacaa agacccgaat ctctccggcg
      481 atgacgttcg cgagggcctc accgccgtgg tgtcggtgaa agtgacgcag ccccagttcg
      541 agagccagac gaagatcaag ctgaacaacc ccgaggttga ggggattgtg aactctgtcg
      601 ttggcgaggg tctctcgatc tacctggagg agcaccccag cgaggcaaag cgcatcatcg
      661 ataaggcact gaccgccttc cgcgcccgtg aggccgcgcg caaggcgagc gagatgatca
      721 agcgccagaa ctacctggag agctccaccc tccccggcaa gctggcagac tgcacggaga
      781 gggatccctc gaagtgcgag ctgttcatcg tcgagggtga ctcggccggc ggctctgcca
      841 agatgggccg cgaccgccgt taccaggcga tcttgcccct gcggggcaag gtgctcaatg
      901 tcgagaagac gcgcatggat cgcgcgctcg ataacgatgc aatcgccgcg ctcattacca
      961 gcatcggcac cggcatctcc aagagcgttc acctgcatgg cgatcaagag gagaacggcg
     1021 acgaggaaaa cggcaacggt cacagcaatg gccgcggcaa tggcaacggc aacgggaatg
     1081 ggaacggcaa ggggcacgat gtcagccgct tcgacatctc aaagctgcgc taccatcgca
     1141 tcatcctgat gaccgatgcc gacgtggatg gcgcgcatat tcgcacgctg ttgctcacgt
     1201 tcctgttccg ctatatgggg ccgcttgtcg agcagggcca tgtctatatc gcgcagcctc
     1261 ctctctacca gattcgcatc gggaagaagg aagactacgc ctatagcgac gaggagctgc
     1321 agcagaagct gaaggcgacg gactcgaaga acgtctccat ccagcgctac aagggtttgg
     1381 gcgagatgaa cgccgaccag ctgcgcgaca cgacgatgga tcccgccacg cgcaccatcc
     1441 ttcaggtgcg cctggaggat gccgtgaagg cggatgagat cttcaccatt ctgatggggg
     1501 acaaagtggg accccgtaag gagttcctcg atgcccacgc gaaggacgtg aaggagctgg
     1561 atatctaggg cacccagacc agcctgagca tcatagagcg cggtcgcccg ggatgggcga
     1621 ccgcgctcat tctctgtatg gcgaacacaa cccggctcat cgaggaaagc atgcgcatcc
     1681 cgcggggcct gccccttatt tctcctggcg cttgcccttc tcgatcagct cgcggacgcg
     1741 cgggccacca cgctcgccac ccttcttacc gatctcctcg taaaactcgt gcccatagcg
     1801 ctctgcggtc gtctcgccgc ccttgcggcc cgcttcacgc acggtcatcc cgccctcttc
     1861 ttgcttcgcc attcgctatc ctccagtcgg gcccgccggg atccgcgagc cgggggcccc
     1921 cacggtatga gccatcgaaa gcccgatcga acccctctcg ccctgccgcc caggcgccgt
     1981 aagccgcccc gcctggcccg cgggaacgag agccctggtc atccgcgagg tgccagcgcg
     2041 cagtggggag ctgcgcgctg caaggacccg aggcgatcac ccacccttct gctcggcctc
     2101 cttgcccttg cggatcagct cgcgcacgcg ctgcccgcct ttgtgaccca gctcctcgta
     2161 gccccggcgg cccagcgcct ttttccggat gttgccaccg attctaccgg cctcacgcac
     2221 ggtcatttcg ccttcggtcc tgctcatgcc cagatctccc tccgctgtca gggtcgttag
     2281 agtcgcccgg ccgccgattc ccatgactcg ctcaaggctt tccttgccgg tcgtggcctc
     2341 cgccgtggcg gaggataccg accgtcaccg gcagagccgt ccggtgggcg gatggggcct
     2401 actgctcctc caattccttc cccttttcaa tcaactcgcg gacgcgttgc cccccacggg
     2461 ggccgccgat ctcgcctcct ttatggccaa tctcctcata gaactcgtgc ccatgcgtgc
     2521 gggccgtggc ctcgccgccc ttgcgacccg cctcgcgcac ggtcatatcg ccttcatcac
     2581 gacgtgccat cgtgccgctc tccctccgcg accggatggt accggtaggc cgggagcggc
     2641 ccctatctcc gctcccggtt cccggcccgt cgccgcaggc tctgcctgaa cggctccgga
     2701 gcgctggatg cgtcgcgcca ttcctgtgac tggcgcacgc ggatcaatcg gcagtgcgtc
     2761 cgctggcaca cctcgcatgc acgacggatg ccaatgcatc gcatcccagg tgcgcccggg
     2821 cacgcaccgc cacagccagg aggtcacttc tcctgcatct tgcccttgtt gatcagctcg
     2881 cgcacgcgct gcccgccctt ctggcccagc tcctcatagc cttcatggcc cagctcgcgc
     2941 ttgcgggcgt tgccaccctt cttgccgatc tcctcgtaga actcatgtcc atgagtacga
     3001 gccgtggtct cgccaccctt gcggcccgcc tcgcgcacag tcattccgcc ctctggcatt
     3061 ctgttcacct cccttcgcaa gggcatagag ccccatcatc acacaccgat tgcttacccg
     3121 cctcgcccga gaggctaaca ggcactgtga ttccattcac accggcgcgc ggcggcggcc
     3181 ccgcgggcac gccgggccca tgccccgaac gagtggaatc agaagccaag gcgggcgcaa
     3241 tcgggaggaa gtgaggagca cagggttgca cagaagtgag gtgatgagtg gcatccggcc
     3301 atgcgccggc gccggcctta cccgggaaag accgtgctca ggaggatgcc ctcggatggc
     3361 gtcgcctggc cagcgccagg gcatgctcga cgatggcccc cgccacgtcg atgccggtcg
     3421 ccgccgccag gccgtgccat tgcggcgcgc cgttcacctc cagaagaacg gggccgtcgg
     3481 ggccggggag aagatcgatg ccggtgtact cgaggccgat caccgacgcc gcccgaaggc
     3541 acaattcgcg ttcataggcg ctcagctcgt agggaacgca cacgcccccc tggtggacgt
     3601 tggtgcgcca ctcggtgccc tgagcgatgc gcatcaccgc gccaaggact tcgccaccaa
     3661 ccacgaagac acg
//
LOCUS       DAOSCV010000416         3671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16012_length_3671_cov_2.531250, whole genome shotgun sequence.
ACCESSION   DAOSCV010000416 DAOSCV010000000
VERSION     DAOSCV010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16012_length_3671_cov_2.531250"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLY56_10785"
     CDS             complement(<1..186)
                     /locus_tag="PLY56_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22011.1"
                     /translation="MLLCTALLLLASMGSARADVTLSNPQVFPNCREAPTNAWVDGTA
                     RSLFTFTITVTVPSTERI"
     gene            complement(700..903)
                     /locus_tag="PLY56_10790"
     CDS             complement(700..903)
                     /locus_tag="PLY56_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22012.1"
                     /translation="MARRQAYSDEERQALQAQLCQYIDECLDVCGKTRGDLHVVHEES
                     QKAIAQISQALKDAGERARAAQL"
     gene            complement(1202..>3671)
                     /locus_tag="PLY56_10795"
     CDS             complement(1202..>3671)
                     /locus_tag="PLY56_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22013.1"
                     /translation="YVTGKVYTKSLPLTAQGEYSFVFKAKEGSVIVTSGDPSKVVVGE
                     PAPPVLEGEQVTEAGGDRFTYRVTYKQAQNLPPAWVRLEIALQGKPETARVVDMVRES
                     PSSASYAGGVVYTYTTPDPLSPGQYTYRITASNGFAEAEMAGEAPLINTPPTLSGWKL
                     SPERGDENTRFTFEVIYTDADNQPPATYTGSDGKQYPSVWVSVGNTLYPLKQADPNDT
                     NYADGALYRVDEMTLDEMTLPAGDLGHSFIASDGHATVRAPASGVVPGPKVLPATKVS
                     MHLSRASVTLDAQTAVTLNGALEPVVPNASVTVTFGVLVEGASPNDVTEETRQTVEVR
                     TDSEGRFSASVVPDQDGTWVVRASYAGDLAEGGSRGSGLGEAQFTVDPARYTLMPGRH
                     MMGVPTTFTGALQDAIAGGEFQTAHYTGDVAVPYLYGPDAALPLPVPGSGFWLRAKAT
                     LQVTAKGRVVDHSAPVTLAIRPGWQIISNPYLLPIDWQATQVRVGGQTLPLMEAGEWV
                     SPFAWTWDPVRGDWRLLHGGYGGLFAQTDRELQPWQGAFVFSRVAGELILAAPSANRA
                     AREDARAMRSRHRASEREWSIQLRSSNGREVDEFNYAGVTAQGRAATHARISTPPRLA
                     GYVDLYFQSTGSSTRYATDFRSPVAGEQTWDFTVETDVLDRPVTVSAPDLSRIPKTLE
                     VLLEDVDAGGKRTYLRTSGGYTFNPGESGTRQMRLIVRPRAQGALRVVQVDVKPTRGG
                     RAFNVTLSQDALVSVAILSPSGQEVAVVADNKATSECRATLFWDETRKDGARVPAGVY
                     LARVTATLPEGQVARSVRMLVLTR"
BASE COUNT          579 a         1287 c         1148 g          657 t
ORIGIN      
        1 gatccgttcg gtgcttggca ccgtcacggt gatcgtgaag gtgaacaggc tccgcgcggt
       61 accatcgacc caggcattgg taggcgcctc ccggcagttg ggaaatacct gcggattgga
      121 gagcgtgaca tccgcgcgcg cggagcccat cgatgccagg agaagaaggg cagtgcacaa
      181 aagcatgccc ccaactcgcg ggagggcggg atgttggttg cggcggcaat ctgcagccac
      241 ggcatccacg gcggacgcga gccgcatcac ccaacgaacg gctatcttca ccggcctcat
      301 tgagctattc cctttccagg gcgacaaagg acttcgatgg gaatggggca tctcacagca
      361 ccacgcttcg acccagggta gagcgctagt tgcaccctcc cctgtttcct ggatcgcgga
      421 cttcgagagt agcaggtgac catggtttga gtggtttccg ggcactgccc ggggccctcg
      481 ggcgaggaga cccggatgga attataccat acgctcgctt gccctgtcaa cgcgcgcctc
      541 atggttgttg ggcacactcg ccgggcgctc ctctttcctg cggcggctgg accgagagcg
      601 tcctcgcttc cccagcgccc caccgctcga aaggatcacg tcctcatcct gggcagccgc
      661 ggtctccgcg ttcagcccgg actccgggcg atccgtcacc tacaactgcg ccgctcgcgc
      721 cctctccccg gcatccttca gcgcctggga gatctgggct atcgccttct ggctctcctc
      781 atgcacgacg tgcaagtcac cacgggtttt gccacacaca tccaggcact catcgatgta
      841 ctgacacagc tgcgcctgca gcgcttgtcg ctcctcatca gagtaagcct ggcgtctggc
      901 cacctggttc tccatcctcg cgcggcatgg ggccgcccgg ttatccgcgg tcagctcgcg
      961 ttccgcatcg agcccataag gactcgctcc gcgtgctcat cacggcaccg tgtgggctcg
     1021 ccctcggggt ttcgccgtca acgggcgagg ttcccccgta tggagcagca tccaccgcgg
     1081 tgccggcctt gcagggaacc aatccactgc tgctgcagcc cgattctctc tcacgtatct
     1141 acgggagcgg gccgcgaggc ccactcccgt cgctatagtt cgccgtgctg ggccgtctct
     1201 cctacctggt cagaacgagc atccggacac tccgcgccac ctggccttct ggcagggtgg
     1261 ctgtcacccg ggccagatag acgccggcag gcacgcgcgc cccatccttg cgcgtctcat
     1321 cccagaagag agtggcgcgg cactcggatg ttgccttgtt atccgccaca accgccactt
     1381 cctggcccga tgggctcagg attgcgacgc tgaccagtgc atcctggctg agcgtgacgt
     1441 tgaacgcccg tccgccgcga gtcggcttca catccacctg caccacgcgc agtgcaccct
     1501 gagcccgcgg tcgaacgatc aggcgcatct gacgcgtccc gctctcgccc gggttgaagg
     1561 tgtagccgcc gctcgttcgc agataggtcc gcttaccgcc agcgtccaca tcctccagca
     1621 gcacttcgag cgtcttcggg atgcgcgaca gatccggcgc actcacggtc accggccggt
     1681 cgagcacatc cgtctcgacg gtgaagtccc acgtctgctc cccagccacc gggctgcgga
     1741 agtcggtcgc ataccgcgtg ctgcttccgg tgctctggaa gtagaggtcg acgtaaccgg
     1801 ccaggcgcgg tggcgtggag atgcgcgcgt gtgtcgcggc gcgcccctgc gcggtgacgc
     1861 cggcatagtt gaactcatcc acctcgcggc cgttgctgga ccggagttgg atcgaccact
     1921 cccgctccga agcgcgatgt cgcgagcgca tggcgcgagc atcctcgcgt gctgcgcggt
     1981 tagcacttgg cgccgccagg atgagctcgc ccgccacgcg gctgaagacg aacgctccct
     2041 gccacggctg cagctcgcgg tctgtctgag cgaacaggcc tccatagccg ccgtgcagca
     2101 ggcgccagtc gccgcgcacc ggatcccacg tccatgcgaa cggcgagacc cactcgcccg
     2161 cttccatgag aggcagggtc tgcccgccaa cgcggacctg cgtggcttgc cagtcgatcg
     2221 gcagcaggta ggggttgctg atgatctgcc aacccggccg gatcgcgagc gtcaccggcg
     2281 cggagtgatc caccacccgg ccctttgctg tcacctggag ggttgccttg gcacgcagcc
     2341 agaagccgct gccgggcacc ggaagcggca acgccgcatc cggaccgtag aggtagggaa
     2401 ctgccacgtc gcccgtataa tgagcggtct ggaactcccc accggcgatg gcgtcttgca
     2461 gcgcacccgt gaacgtggtg ggcacgccca tcatgtgccg cccaggcatc agcgtatagc
     2521 gcgccgggtc caccgtgaac tgggcctcgc ccaggccgga gccgcggctg ccgccctccg
     2581 ccaggtcacc cgcgtagctg gcgcggacca cccacgtccc atcctggtcg ggcacgacag
     2641 aggccgagaa gcgaccctcg ctgtccgtgc gtacctcgac ggtctgtcgc gtctcttccg
     2701 tcacgtcgtt gggcgacgcg ccttccacga gcacgccgaa ggttaccgtc acgctagcgt
     2761 tcggcaccac cggctccagg gcgccgttca gcgtaaccgc cgtctgagca tcaagggtca
     2821 cgctggcccg gctgagatgc atgctcacct tcgttgccgg cagcaccttc gggccgggga
     2881 ccactccgct tgccggggcg cgcaccgtcg catgcccatc actggcgatg aaggagtgcc
     2941 ctaggtcgcc cgcaggcagc gtcatctcgt ccagcgtcat ctcgtcgacg cggtagagcg
     3001 cgccatcggc gtagttggtg tcgttcggat ccgcctgctt cagcgggtac agggtgttgc
     3061 caacgctcac ccacacactt ggatactgct tgccgtcgga gccggtgtat gttgccggcg
     3121 gctggttatc ggcatcggtg tagatcacct cgaaggtgaa gcgcgtgttc tcatcgccgc
     3181 gctccgggct cagcttccag cccgagagcg tgggcggcgt gttgatcaag ggcgcctcac
     3241 ccgccatctc cgcctccgcg aagccgttgg aggcagtgat gcggtaggtg tactggccgg
     3301 ggctcagcgg atccggtgtg gtgtaggtgt aaaccacccc acccgcatag gacgcgctgc
     3361 tcgggctctc gcgcaccatg tcgaccacgc gcgcggtctc cggcttgccc tgcagggcga
     3421 tttcgagccg cacccatgcc ggcggcaggt tctgcgcctg cttgtaagtc acgcgatagg
     3481 tgaaccggtc accacccgcc tcggttacct gctctccctc cagcaccggc ggggccggct
     3541 cgcccacgac cactttagag ggatcacccg aggtaacgat cacactcccc tctttcgctt
     3601 tgaagacgaa ggagtactcg ccctgggcgg tgaggggcag gctcttggtg tagaccttgc
     3661 cggtcacgta g
//
LOCUS       DAOSCV010000417         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16050_length_3665_cov_3.459834, whole genome shotgun sequence.
ACCESSION   DAOSCV010000417 DAOSCV010000000
VERSION     DAOSCV010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16050_length_3665_cov_3.459834"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2508
                     /locus_tag="PLY56_10800"
     CDS             247..2508
                     /locus_tag="PLY56_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOJ22014.1"
                     /translation="MSQAEDSVAIPLASGGVMRVQPVAAHTFRIRLRPDAAFAEPALV
                     RYGILRSEWPAVEFTTGGDEETAFVRAAEAELHVRRSDGWLTLCDASGRPVVESAGAP
                     RSDTESGFGAEFALQEDERLYGLGDVTRERIQKRGNRSQIWVVNVKSYVPIPYVMSTR
                     GWALFVNSTWRHTFDLGCRVPDRLCFWGKRGELDFYLILGDSAPQLLDRYTDIVGKPA
                     LLPLWAYGLTFVCNQQANAREMLDDCLNFRREGIPCDVIGLEPGWMEKHYDFSVEKKW
                     HPERFFIPSWSPKGPHTFLSAAGRLGFKMSLWLCCDYDLSYEEERQLQAKSAVQAEAE
                     SRAPDADDFEQDEHFAHGRTLMDRMTRPEEPWFEHLKKFVGQGVSAFKMDGALMVNEH
                     PDRKWGNGMDDEEMHNLYPVLLNKQMSRGFAEHTGRRPMVYSSGGYAGIQQFSATWAG
                     DTGGGPKPLVSMLNHGLSGHVNTSCDMDVFTPAGIHFGFLQPWPQLCSWAYWRHPWFL
                     GKDLFPIFKWYARLRYRLIPYIYSMAHVAARTGMPIMRAMPLVYPDHPGADELLQQYL
                     FGDAFLVAAFTNRVSLPAGKWIDFWTGEAHEGPKEMECQVPADRGGPLFIRAGSIIPN
                     WPEMQYVGEQPVDTLSLHVYPHGESEFTLYEDDGVSFGYREGQVAQTRITCRAARDQV
                     ELEIAPRVGAYEGMPDERCFDVWLYTESAPASAWINGSAAEWCYDEDARAVRLRVAED
                     PDRRRPALVRCVW"
     gene            2639..>3665
                     /locus_tag="PLY56_10805"
     CDS             2639..>3665
                     /locus_tag="PLY56_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22015.1"
                     /translation="MKRLWLWRGVVLPALLALMVWPAGAAQGPEAWERPGENIALGKS
                     YTLVPRPNYALCRDDGDRTQLTDGQYTVGYFWTQQTTVGWSGGGQTRIVIDLGQVEPI
                     VGVSFHTAAGRAEVRWPRSLLLFVSDDGETWWRAGDLVKLDRGPEPPADTYATRRYWT
                     NQLATRGRYVAVVMVPTGSYAFCDEIEVYRGDQAFLTRPREGRAVADIDAAITALRFE
                     EDVRAEAEALRERLGAARIAAGQKTRIQTMLDQAVVNLNEIEGVGGNERAIWPYGKAH
                     EKLLAVQAALWRAEGRPALQAWAGSCWDPLLPWQAPERTAAANIDLVMMRNEVRSAAI
                     NITNANDA"
BASE COUNT          620 a         1127 c         1276 g          642 t
ORIGIN      
        1 gggcgagcac acgatccgca tgaccaatct gggcgatggc ctggcgctcg actggctcgc
       61 cctgatccgc gaaaagtagc gaagactgtc aggcgccggt cgccatcctg ctcatggggt
      121 ggcgaccggc gcttcgattg gtgtcgcgca tccgtcggcc tcgggcccaa accaactgcc
      181 cgagggcgtg ccaatccacc ggactgctcg ccaggggaga gcccgtcggg ggaggaagtc
      241 aagacgatgt cgcaggctga agactctgtt gcgattccgc ttgcatcggg aggggtgatg
      301 cgcgtgcagc cggtggcggc gcacacgttt cgcattcgcc tgcgtcctga tgctgccttc
      361 gcggagccgg cactcgtgcg ctatgggatt ctccggtcgg agtggcccgc ggtcgagttc
      421 accaccggcg gcgacgagga gaccgccttc gttcgcgccg cggaggccga gctgcacgtg
      481 cgacgttcgg acggctggtt gacactgtgc gatgccagcg ggcgcccggt ggttgagagc
      541 gccggcgcgc cacgctccga cacggaaagc ggcttcggcg cggagttcgc cttgcaggag
      601 gatgagcgcc tctatggcct gggcgatgtg acccgcgaac gcatccagaa acggggcaac
      661 cgctcccaga tctgggtagt gaacgtgaag tcctacgtgc ccatccccta tgtgatgagc
      721 acccgcggct gggccctttt cgtgaactcc acctggcgcc atacttttga cttgggttgc
      781 cgcgtgccgg accggctctg cttctggggc aagcgcggcg agctggactt ctacctgatc
      841 ctcggagaca gcgccccgca actcctcgac cgctataccg acattgtggg caagccggcg
      901 ctgcttccac tctgggctta tgggctcacc ttcgtctgta accagcaggc gaatgcccgt
      961 gagatgttgg atgactgcct caacttccgc cgggaaggga tcccctgcga tgtgatcggg
     1021 ctcgagccgg gctggatgga gaagcattac gatttttccg tggagaagaa gtggcacccc
     1081 gagcgcttct ttatcccatc ctggagtccc aagggaccgc ataccttcct gagcgcggca
     1141 ggccggctgg gcttcaagat gagcctctgg ctgtgctgcg actatgatct cagctacgaa
     1201 gaggagcgcc agcttcaagc gaagagcgcc gtccaggctg aggcagaatc gcgcgcgccg
     1261 gacgcggatg acttcgagca ggacgagcac tttgcgcacg ggcgcacgct gatggaccgc
     1321 atgacgcggc cagaggagcc gtggttcgag cacctgaaga agttcgtcgg tcagggcgtc
     1381 tccgcgttca agatggacgg cgcgttgatg gtgaacgagc atcctgaccg caagtggggc
     1441 aacggcatgg atgacgagga gatgcacaac ctctatccgg tgctcctcaa caagcagatg
     1501 agccggggtt tcgctgaaca caccgggcgc cggcccatgg tctacagctc cggcggctac
     1561 gctgggatcc agcagttcag cgccacctgg gcgggtgata ccggaggcgg gcccaagccg
     1621 ctggtctcta tgctcaacca cggtctctcc ggccatgtta acaccagctg cgacatggac
     1681 gtgttcacgc cggccgggat ccatttcggc ttccttcagc cctggccaca actgtgcagc
     1741 tgggcctatt ggcgccaccc gtggttcctc ggcaaggatc tcttcccgat cttcaagtgg
     1801 tacgcccggc tgcggtatcg cctcatcccc tatatctact cgatggcgca cgtcgccgcg
     1861 cgtaccggca tgccgatcat gcgcgccatg cccctggtct atcccgacca tccgggtgcc
     1921 gatgaactgc tgcagcagta ccttttcggc gatgctttcc tcgtcgcggc gttcaccaac
     1981 cgcgtctctc ttccggcagg gaagtggatc gacttctgga cgggcgaggc gcacgagggg
     2041 cccaaagaga tggaatgcca ggtgccggca gaccggggtg ggccgctctt catccgagct
     2101 ggaagcatca tcccgaactg gccggagatg cagtacgttg gtgagcagcc ggtcgatacg
     2161 ctcagcctgc acgtctaccc gcatggcgag agcgagttca cgctctacga ggatgacggc
     2221 gtcagcttcg ggtatcgcga gggccaggtt gcgcagacgc gcatcacctg ccgcgccgcg
     2281 cgcgatcagg tggagctgga gattgccccg cgtgttggcg cctacgaggg gatgccggac
     2341 gagcgttgct tcgacgtgtg gctctacacc gagagcgcgc ctgcgtccgc atggatcaat
     2401 ggcagcgccg ccgaatggtg ctacgatgag gatgcgcgcg cggttcgcct gcgcgtggca
     2461 gaggatccgg atcggcggcg gcctgccctg gtccgttgtg tctggtgaga aatgctgtgg
     2521 cgatgaggcc ggacgggcgc gcccgatgga gcaggggcgt gctctgccgg tcaagtgcca
     2581 tgtgggcatc cggattgact gggtgcccaa tagcctgtag gaatggagtg aggactttat
     2641 gaagcggtta tggctatgga gaggggtcgt tctgccggct cttctcgccc tgatggtgtg
     2701 gcccgcgggg gccgcccagg gtccggaggc ctgggagcgt ccgggcgaga atatcgcact
     2761 gggcaagagc tacactctgg tgccgcgccc gaactacgcg ctgtgccggg atgatggaga
     2821 cagaacgcag ttgaccgatg gccagtacac cgtcggctac ttctggaccc aacagaccac
     2881 cgtggggtgg agcggcggcg ggcagacgcg catcgtcatc gacctgggcc aggtggagcc
     2941 gattgttggc gtgagcttcc atacggcggc gggacgcgcg gaagtgcgct ggccgcggtc
     3001 gctcctcctc tttgtctcgg acgatggcga gacgtggtgg cgcgcgggcg atctggtcaa
     3061 gctcgaccgg ggccccgaac cgcctgccga tacctatgcc acgcggcgct actggaccaa
     3121 ccagctggcg acccggggcc gctacgtggc ggtcgtcatg gtgccaactg gcagctacgc
     3181 cttctgcgac gagattgagg tctaccgggg cgatcaggcg tttctgacgc gcccgcgcga
     3241 gggcagggca gtggccgaca tcgatgcggc gatcacggcg cttcgcttcg aggaggacgt
     3301 tcgagcggag gccgaggcgc ttcgtgagcg cctcggggcg gcgaggatcg ccgccggaca
     3361 gaagacacgc atccagacga tgctcgacca ggcggtcgtc aatctgaacg agatcgaagg
     3421 ggttggcggc aacgagcggg ccatctggcc gtacggcaag gcgcacgaga agctgttggc
     3481 ggtccaggcg gccctctggc gcgccgaggg gcgcccggcg ctgcaagcgt gggccgggtc
     3541 gtgctgggat ccccttctgc cctggcaggc gccagagcgg accgcagcgg cgaacatcga
     3601 tctggtgatg atgcgcaacg aggtgcgcag cgcggcaatc aacatcacca acgcgaacga
     3661 cgcgg
//
LOCUS       DAOSCV010000418         3662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16060_length_3662_cov_3.617688, whole genome shotgun sequence.
ACCESSION   DAOSCV010000418 DAOSCV010000000
VERSION     DAOSCV010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16060_length_3662_cov_3.617688"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLY56_10810"
     CDS             <1..468
                     /locus_tag="PLY56_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22016.1"
                     /translation="FTWLDFPPGMEENGDIPYREMTELVKLHHEDVSHIRVFDGVEVT
                     EENLGNLPREYLGKKFLGLSKAQPWWQLPVEFAGVPNGHDGTHVFLVNDFVRSVKSHR
                     LPPNHVWLAARYNAPGIVAHESCRRGGELMKIPDFGMPPADWPTLEETHPLQP"
     gene            629..1504
                     /locus_tag="PLY56_10815"
     CDS             629..1504
                     /locus_tag="PLY56_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22017.1"
                     /translation="MKIGVSSYSYSRLVRTGAMKQIEVIAAAKEAGFDVIEFSTIAVP
                     EGKTLPGFAEELRKEADRVGLPIANYTIAADFLNGSDGNLEAEIARVKAEVDVAEILG
                     VPGMRHDATRGFAADYTGPKSFDAAVSRLTEGCRAVTEYAASKGIKTMVENHGFFCQD
                     SDRVEKLVTAVAHPNFGVLIDIGNFACADEDSQIAVGRLAPYAFHAHAKDFHIRPGYL
                     PAPGEGWFMSRAGNYLRGAIIGHGDIPLLGCIRALKRAGFDGVLSIEFEGMEDVLTGI
                     RIGRDNLVRLVEMAG"
     gene            1611..>3662
                     /locus_tag="PLY56_10820"
     CDS             1611..>3662
                     /locus_tag="PLY56_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22018.1"
                     /translation="MKQRGILVLLACLLIFPSVCRAHWLDHQELPEWARRGYCQWGHG
                     ANINGRIHWTENGYGVDLPNIHLIRYCGRNLMQTITYLDEEARRVGESAGVRRQPYIC
                     SKTIWWRNEFPKAPQLERCTILKPDGTRVLLYNNPERYGGCYSSPIWLDYVKQRVDSL
                     LAKPGGEVHSIFFDNCMNYDCHCAECHARFKEYTREKFGREMALSPSDKSPDYLFARR
                     MFDADEAVRFFQEIRRHLNTRHREGILISPNIGIGYGWSSYLVNRGATDLVFIEEGFT
                     FPPFESTVLKYKLGLAASHGKTVGQLLGLPQGLRRQRALALDEKHEMGILEAFVYPEE
                     HKLALAEAMATNGTNCVSFALREQKITANDAPYQVQNREAIHQYSAFHQRHLPLFDRA
                     QPAARVAVVHPVVTELGRYNTRTALQQATRALERAGVPYEVLVEEDLEPEQLRHYRLL
                     ILPEVYCLNRERCQALEEWLKRGGALVQLGSLAQADELGRAYPAGRLPLVGHLAEADP
                     AEIGAGRVWLPSRSLDEMPARTFLAELQRLAGPLERAETRSPRLFCNLLRSRDGKSLT
                     AHLVNSDFSYTLPPTGDIQDDDGLSEARTFLSTPAARIRKVLRVPEPAKARDLYLKFV
                     SATCGVATDAFSLVVTFNGQEIATIPGSWLNDGPGEGIPVPPGLIREENEVVFRVTGR
                     PN"
BASE COUNT          629 a         1169 c         1252 g          612 t
ORIGIN      
        1 ttcacctggc tcgacttccc gcccggcatg gaggagaacg gcgacattcc ctaccgtgag
       61 atgacggagc tggtgaagct gcaccacgag gatgtgagcc atattcgcgt cttcgacggc
      121 gtggaggtca ccgaggagaa cctgggcaac ctgccgcgcg agtacctggg caagaagttc
      181 ctaggtctct cgaaggcgca gccgtggtgg cagctcccgg tggagtttgc cggggtgccg
      241 aacgggcacg acggcacgca tgtcttcctg gtgaacgact tcgttcgctc ggtgaagagc
      301 caccggctgc cgcccaacca cgtctggctc gcggcgcgct acaacgcgcc cgggattgtg
      361 gcgcacgagt cgtgccggcg cggcggcgag ctgatgaaga tcccggattt cggcatgccc
      421 ccggccgatt ggccaaccct ggaggagacg cacccgctgc aaccgtgaga gcgggcgcgg
      481 cgttccgaga gccggcgcgg gaatgattcc ggcccgagcc catcgaacat tagaggcaac
      541 gggagaccga tgggtggacc ccgttttgca gcgtggcatg ggcgcggggc gaagcccttt
      601 gcccgggtgt cagcaaggga gatccagagt gaagattggt gtcagttcct acagctatag
      661 ccggttggtc cgcaccggcg cgatgaagca gatcgaagtg atcgccgccg ccaaggaggc
      721 gggcttcgat gtgatcgagt tcagcaccat cgccgtgcct gaaggcaaga cgcttcccgg
      781 attcgccgag gagctgcgca aggaagcgga tcgcgtgggc ctgccgattg ccaactacac
      841 catcgccgcc gacttcctga acggctcgga tggcaacctg gaggcggaga tcgctcgcgt
      901 caaggccgaa gtagatgtgg cggagatcct cggcgtgccc gggatgcggc acgacgccac
      961 gcgcggcttt gccgccgatt acacggggcc aaagagcttt gacgcggctg tgtcgcgtct
     1021 gaccgaaggg tgccgcgcgg tgacggagta tgccgcctcc aagggcatca agacgatggt
     1081 ggagaaccac ggcttcttct gccaggatag cgaccgcgtc gagaagctgg tcaccgccgt
     1141 ggcacacccc aacttcggcg tgctgatcga catcggcaac ttcgcctgcg cggatgagga
     1201 ctcgcagatc gccgtcgggc ggctggcgcc ctacgcgttc catgcgcatg ccaaggactt
     1261 ccacatccgg ccgggctatc tccccgcgcc cggcgagggg tggttcatga gccgcgccgg
     1321 caactacctc cggggtgcga tcatcggcca cggcgatatc ccgctcctgg gatgcatccg
     1381 cgcgctgaag cgcgccggct tcgatggcgt cctctccatc gagtttgagg ggatggagga
     1441 cgtgctcacc ggcatccgga tcgggcgaga caacctagtg cgcctagtgg agatggcggg
     1501 ctagcatccg ctcgccacga ttcaccggga agctcgtggg gcgtggggga tggccgggct
     1561 ttcccccgcg cctcgggctc tgcggcgctc ccgcgcggga ggctcgtacc atgaagcagc
     1621 gtggcatcct ggtgttgctt gcctgtttgc tgatcttccc ctcggtttgc cgggcgcatt
     1681 ggctggatca ccaggagctc ccggagtggg cccggcgcgg ctactgccag tggggccatg
     1741 gcgccaacat caacggccgg attcactgga ccgagaacgg ctacggtgtc gatctgccca
     1801 acatccacct gatccgctac tgcgggcgca acctgatgca gacgatcacc tatctggatg
     1861 aggaagcccg gcgcgtgggc gagagcgctg gggtgcggcg ccagccctac atctgcagca
     1921 agacgatctg gtggcgcaac gagttcccca aagcccccca actggagcgg tgcaccatcc
     1981 tcaagccgga cggcacgcgc gtcttgctct acaacaaccc cgagcgctac ggagggtgct
     2041 acagcagccc gatctggctg gattacgtga agcagcgcgt ggatagcctc ctggcgaagc
     2101 ccgggggcga ggttcactcc atcttctttg acaactgcat gaactatgat tgccactgcg
     2161 ccgagtgtca cgcgcgcttc aaggagtaca cccgcgagaa gttcgggcgc gagatggcgc
     2221 tctcgccctc ggataaatcg cccgattacc tgtttgcccg gcggatgttc gacgccgatg
     2281 aggcggtgcg cttcttccag gagattcgcc gtcacctcaa cacccggcac cgcgagggga
     2341 ttctgatctc gcccaatatc ggcattggct acggctggag cagctatctc gtcaaccggg
     2401 gcgcgaccga cctggtcttc atcgaggagg gtttcacgtt tccgccgttc gagagcaccg
     2461 tgctgaagta taagctgggc ctcgctgcgt cgcatggcaa gacggtgggc cagctgctcg
     2521 ggctcccgca gggactgcgc cgacagcgcg cgctcgccct ggacgagaag catgagatgg
     2581 gcatcctcga ggcgtttgtc taccccgagg agcacaagct cgccctggcc gaggcgatgg
     2641 caacgaacgg cacgaattgc gtctcattcg ccctgcgcga gcagaagatc acggcgaacg
     2701 acgcgcccta ccaggtgcag aaccgcgagg cgatccacca gtacagcgcg ttccatcagc
     2761 gccacctgcc gctttttgac cgggcgcagc cggcggcgcg ggtcgcggtt gttcacccgg
     2821 tggtcacgga gctgggccgg tacaacacgc ggaccgcttt gcagcaggcc acgcgggccc
     2881 tggagagggc cggcgtgccg tatgaggtgc tcgtcgagga ggacctggag ccggagcaac
     2941 tgcgtcacta ccggctgttg atcctgccgg aagtctattg cctcaaccgg gagcgatgcc
     3001 aggctctgga agagtggctg aagcggggcg gggcgttggt acagttgggc tcgttggcgc
     3061 aggcagacga gctgggccgg gcctaccctg ccgggcggct gccgctggtg gggcatctgg
     3121 ccgaggccga tccggcggag ataggggcgg gccgcgtgtg gctgccctcg cgctcgctcg
     3181 acgagatgcc ggcgcgcacc ttcctggccg agctccaacg cctggccggc ccgctggaga
     3241 gagccgagac gcgctcgcca cgcctcttct gcaacctcct gcgcagccgc gatggcaaga
     3301 gcctcacggc gcacctggtc aactcggact tcagttacac tcttccgccc acgggcgata
     3361 ttcaggacga cgatggcctg tcggaggcgc gcaccttcct ctcgacgccc gcggcccgga
     3421 tacgcaaggt gttgagggtg ccggaacccg cgaaggcgcg agacctctac ctcaagttcg
     3481 tctcggccac gtgcggggtg gccaccgatg ccttctctct ggtggtgacg ttcaacgggc
     3541 aagagatcgc cacgatcccc ggctcctggc taaatgacgg gccgggcgag gggatccccg
     3601 tgccccctgg cctgatccgc gaggagaacg aggtggtgtt ccgggtgacc ggcaggccga
     3661 ac
//
LOCUS       DAOSCV010000419         3657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16089_length_3657_cov_3.966963, whole genome shotgun sequence.
ACCESSION   DAOSCV010000419 DAOSCV010000000
VERSION     DAOSCV010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16089_length_3657_cov_3.966963"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="PLY56_10825"
     CDS             <1..1942
                     /locus_tag="PLY56_10825"
                     /inference="COORDINATES: protein motif:HMM:NF027656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4962 domain-containing protein"
                     /protein_id="HOJ22019.1"
                     /translation="LRERARGDLTPQFDRLVQRCEQLLQSPPPTAEPPKYPPGTVRNS
                     EEWRKIWWGNRTYTVAALDSAATLAFTRLIGGKEEYGQLARRILMECAKWDPKGSTGY
                     IYNDEAGMPYNWGFSRTYTFVYDLLTEEERAICRRVMKIRGDEMYRHLYPRHLWRPYN
                     SHSNRAWHKLGEIGIAFLGEVEGAEDWVWFAANVFANVYPVWSDDDGGWHEGMAYWAS
                     YLERFSFWADTMKPVLGLDAYKKPFFSQVGYYPMYLMPPGTTGGGFGDLTPERKAPSN
                     ARLVATFAAQAGNGHWQWYVEQVGGSPSTGGYIGFVRGTLPPVKPVPPDDLPTSRLFK
                     GTGLAVLNTSLKHAKENVRVLFKSSPFGTQSHGYEANNAFSLIAYGERLLVQTGRRDS
                     YGTPHHRDWMWSTRSVNAITVDGHGQVPHSAASRGEITAFKTTPAVDIVVGEAGDTYR
                     VVPTAQEREQGAQERKLLDRFTRTLVFLKPDVLVVYDRLVAKQPSTFEYWLHAVNQFE
                     TLAPNSLRTQTGDVACDIDILTPAGLKFHQTNEYDPNPRPRITIREWHLTATTPEKRQ
                     QVEFVTLCAPRKASEAPLPKPTLESVDGGYVLRVRLRDGEATVLLPAREGAVLRADGL
                     ESRGAIVVQRATPRGRTRVEVP"
     gene            2177..3325
                     /locus_tag="PLY56_10830"
     CDS             2177..3325
                     /locus_tag="PLY56_10830"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ22020.1"
                     /translation="MAEPRLRLARHAAPEEWAKYETAHRQFQGIPGIERMPDGRLWAT
                     WYSGGTGEGPENFVLLVTSQDDGLTWSEPLAVVDPPGHTRAFDPCLWRDPLGRLWWFW
                     AESDSPKMGEIMDGRGGVWAARLEGESPEELKFTRPVRIANGVMMNKPTVLSNGEWLL
                     PTAVWACWHVKAEDLAGERFSNATVSTDAGESFTLRGGADVPDRCFDEHLFVELNDGR
                     VWCLVRTFYGIGQSFSSDQGRTWTPGEDSGLGGPNSRFFIRRLASGRLLLVNHIDVTA
                     MESGQAWRAGRSWRRRSHLAALLTEDEGATWIGPLLLDERDGVSYPDGVQAEDGRIFI
                     IYDHDRAGAGEILLACFREEDILAGACVSPDAFLRRLVNRTGGVQSPR"
     gene            3343..>3657
                     /locus_tag="PLY56_10835"
     CDS             3343..>3657
                     /locus_tag="PLY56_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22021.1"
                     /translation="MRKFWMACLAVLLACAGACLAGEARQPRTLYVNNVTGSDRFDGL
                     RPDPAGDGVSGPFASIAKAFASVETSDRIEIANTGKPYAGGNTLVKAGGTATQPLVIN
                     GNG"
BASE COUNT          603 a         1179 c         1305 g          570 t
ORIGIN      
        1 gctgcgcgag cgcgcgcgcg gcgacctcac gccgcagttc gaccgcctcg tccagcggtg
       61 cgagcagctg ctccagtcgc ccccgccgac ggcggagccg ccgaagtacc ccccgggcac
      121 ggtgcgcaac agcgaggagt ggcgcaagat ctggtgggga aaccgcacct ataccgttgc
      181 ggcgctcgat agtgcggcga cgcttgcctt cacccggctc atcggtggca aggaggagta
      241 cggccagctc gcgcggcgga tcctgatgga gtgcgcgaag tgggatccta aggggagcac
      301 cggctacatc tataacgacg aggccggcat gccctacaac tggggcttta gccgcaccta
      361 caccttcgtt tacgacctcc tcaccgagga agagcgtgcc atctgccgcc gcgtgatgaa
      421 gatccgcggc gatgagatgt accggcatct ttatccgcgc cacctgtgga gaccctacaa
      481 cagccacagc aaccgcgcct ggcacaagct gggcgagatc gggatcgctt tcctgggcga
      541 ggtggaaggc gccgaggact gggtgtggtt tgcggccaac gtcttcgcca acgtctaccc
      601 tgtctggtcg gatgatgatg gcggctggca cgaggggatg gcgtattggg cgagctacct
      661 ggagcggttc agcttctggg cggatacgat gaagccggtc ctggggctgg atgcctacaa
      721 aaagccgttc ttttcgcagg tgggctatta cccgatgtac ctcatgccgc ccgggaccac
      781 cggcggcggg tttggcgacc tcacccccga gcgcaaggcg ccttccaacg cgcgtctggt
      841 ggccacgttc gccgcgcagg cgggaaacgg ccactggcaa tggtacgtgg agcaggtggg
      901 gggaagcccc tcgactggag gctacatcgg cttcgtgcgc ggcacgctcc ccccggtgaa
      961 accggtgccg cccgatgacc ttcccacctc gcgccttttc aagggcacgg gcctggcggt
     1021 tctgaacacc tccctgaagc acgcgaagga gaacgtacgc gtcctcttca aatcgagccc
     1081 attcggcacg cagtcgcacg gctacgaggc gaacaacgcc ttctcactga tcgcctacgg
     1141 cgagcgcctc ctggtgcaga ccggccggcg cgattcctat ggcacgccgc accacagaga
     1201 ctggatgtgg tcgacgcgct cggtcaacgc gatcaccgtg gatggccacg gccaggtgcc
     1261 ccactcggcg gcctcgcgcg gtgagatcac cgcgtttaag acgacgccgg cggtcgatat
     1321 cgtcgtcggc gaggcgggcg acacctatcg cgtggtgccc accgcccaag agagggagca
     1381 gggggcccag gagcgcaagc tgcttgaccg gttcacgcgc acgctcgtct tcctgaagcc
     1441 cgatgttctg gtggtctacg accgcctggt ggcaaagcag ccttccacct ttgagtactg
     1501 gctgcacgcc gtcaaccagt ttgagacgct cgctccgaac tcgctccgaa cgcagacggg
     1561 cgacgtcgcc tgcgacatcg acatcctgac gccggccggg ctgaagttcc accagacgaa
     1621 cgagtatgac ccgaacccgc gcccgaggat cacgattcgc gagtggcacc tgacggcgac
     1681 gacccccgag aagcgccagc aggtggagtt cgttaccctc tgcgcgccgc gcaaggcatc
     1741 cgaggccccg ctgcccaagc ccaccttgga gagtgtcgac ggcggctatg tgctccgcgt
     1801 ccggctccgc gatggcgagg cgacggtgct gctccccgcg cgcgagggcg ccgtactgag
     1861 agccgatggc ctggagagcc ggggcgcgat cgtcgtccag cgcgccactc cccgggggcg
     1921 cacgcgggtg gaggtgccgt agctccaggg gtgacgggca acccggcgcg aggaggccca
     1981 cctcctcgct gcttgtgagc gaagccctgt cgggctgggt tggattgcac gtccgggcgc
     2041 ctgagagcgg caagggctgg gagggggagc cccgaaggag ctttgccgga aagcagcgcg
     2101 gagcttcgag ctccgcgccc ggggtggacc aggcgcctgg gcgagccccg aggtactcgc
     2161 gagcgaaagg agcgcgatgg cagaaccacg gctacgcctg gcgcggcacg ccgcgccgga
     2221 ggagtgggcg aagtacgaga cggcccaccg gcagttccag gggattcccg ggatcgagcg
     2281 gatgccggat ggccggctgt gggccacgtg gtactcaggc ggcacgggcg aaggcccgga
     2341 gaatttcgtt ctgctcgtga cgagccagga cgacggcctc acctggagcg agccactcgc
     2401 cgtcgtggat cccccgggcc acacgcgcgc cttcgacccg tgcctctggc gcgacccgct
     2461 cggccggctc tggtggttct gggcggagag cgatagcccg aagatgggcg agatcatgga
     2521 tggccgcggg ggcgtgtggg ccgcccggct ggagggggag tcgcccgagg agctcaagtt
     2581 cacccgcccg gtccggatcg ccaatggcgt catgatgaac aagcccacgg tgctttccaa
     2641 cggcgagtgg ctcctcccga cggcggtgtg ggcgtgctgg cacgtgaagg cggaggatct
     2701 ggccggggag cgcttctcga acgctaccgt ctcgaccgat gccggggaga gcttcaccct
     2761 ccgcgggggc gccgacgtgc cggatcgctg ctttgacgag catcttttcg tggagctcaa
     2821 cgatggccgg gtgtggtgtc tggtgcgcac cttctatggc atcggccaga gcttctcgag
     2881 cgaccagggg cggacctgga ccccgggcga ggactccggg ctgggcgggc cgaactcgcg
     2941 cttcttcatc cgccggctgg cttccgggcg gctgctcctg gtgaaccaca tcgatgtcac
     3001 cgcaatggaa tccgggcagg cctggcgcgc cggaaggtcg tggcgccggc gctcacacct
     3061 ggccgcgctc ctcaccgagg atgagggcgc gacctggatc ggcccgctgc tcctggacga
     3121 gcgcgacggc gtctcctatc ccgatggcgt gcaggccgaa gacgggcgca tctttatcat
     3181 ttacgatcac gatcgcgccg gggcggggga gatcctcctg gcgtgcttcc gcgaagagga
     3241 tattctggcg ggagcatgtg tgtcgcccga tgctttcttg cggcggttgg tgaaccggac
     3301 cggaggcgtg caaagccctc ggtgagagtg gggagaagaa gcgtgcgaaa gttctggatg
     3361 gcgtgcctgg cagtgctgct ggcctgcgcg ggggcgtgcc tggccggcga ggcgaggcaa
     3421 ccgcggacgc tctacgtcaa caacgtcacg gggagcgacc gctttgatgg gttgaggccc
     3481 gatcccgctg gcgacggcgt gagcgggccg ttcgcgagca tcgcgaaggc gtttgcctcg
     3541 gtggagacgt cggaccggat tgagattgcc aacacgggga agccctacgc gggcggcaac
     3601 accctcgtca aggcgggcgg cacggcgacg caaccgctcg tcatcaacgg caacggc
//
LOCUS       DAOSCV010000420         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16111_length_3654_cov_3.524312, whole genome shotgun sequence.
ACCESSION   DAOSCV010000420 DAOSCV010000000
VERSION     DAOSCV010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16111_length_3654_cov_3.524312"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLY56_10840"
     CDS             complement(<1..1205)
                     /locus_tag="PLY56_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22022.1"
                     /translation="MYSRILCAALIIASLVPAAAEAPPKKLIEYGWDVPPPSFVATHI
                     REMEKQPFEGVIVRVPNIGTVFANKKWDESQVAAELAALERIEWRKFTDNFVIMYAAS
                     SMDWFSDADWECVLHNVALCAKAAKLGRCKGLCFDAEPYGKNPWHYPSQARAAEKSFA
                     EYQAMARRRGAQFMAKIQEILPAPVIHTFFQLSYFPSVASLSDPAERERRLAREYYGL
                     LPAFLNGMLDVANPGTRITDGNESSYYYTTAEEYFRAFHNIKQTALSLVAPENRAKYL
                     SQVQCSQALYVDYLFNYWSRPTPAMYMNPEERARWFEHNVYWALKTTDEYVWLYSEKM
                     NWWKGENIPHGLPEAIVAAKEKLAAQQPLGFDMAAIITRAKAAEEKEIRARLEDRQAR
                     IQRIGSRERP"
     gene            complement(1508..2383)
                     /locus_tag="PLY56_10845"
     CDS             complement(1508..2383)
                     /locus_tag="PLY56_10845"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ22023.1"
                     /translation="MRRTVCLAGIMLLGLLPGARADRSSVVYERKVILGLPVNVITVN
                     LNDPDVCVTAAIAKGGIGTSENFKSLINRVHPAAAITGTFFDTRSLVPVGDIVVAGQQ
                     VHQGMVGTGVAFAGNRIDFVPLRAGRKEDWAPYEMVLCAGPWLVKNGVRTVRPWAEGF
                     RDASLYARRPRAAIGLTKYNKLLLVTIDRPVYYTHVSKVMKALGAVNALGMDGGSSTA
                     LSIRGRIISKPSRRLTNVLLVYDKPQAYERAIARNRLVPNLRRKPVAPLAPLGDALHP
                     PVNSPLMEVLIEPAG"
     gene            complement(2574..2894)
                     /locus_tag="PLY56_10850"
     CDS             complement(2574..2894)
                     /locus_tag="PLY56_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ22024.1"
                     /translation="MAECEEGTCLVECRGEVDIASAPKFRAALDEGLEKATRRLVIDL
                     RGVSFMDSVGVGQIVGAYKRLREGAQLQICTENDHIRTLLEIAGLSGVSSVHRSKEEA
                     LAVR"
     regulatory      complement(3291..3424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          626 a         1182 c         1161 g          685 t
ORIGIN      
        1 gggcgctcgc gcgagccgat ccgctggatt cgcgcctggc ggtcttccag gcgcgcccga
       61 atctccttct cctcggccgc cttcgcgcgg gtgatgatcg ccgccatgtc gaatccgaga
      121 ggctgctgcg cggccagctt ctccttggcc gcgacgattg cctcggggag accgtgcggg
      181 atgttctccc ccttccacca gttcatcttc tcgctataga gccagacata ctcgtcggtg
      241 gtcttcagcg cccagtagac gttgtgctcg aaccagcgcg cgcgctcctc ggggttcatg
      301 tacatcgccg gcgtgggccg ggaccagtag ttgaagagat agtccacgta aagcgcctgg
      361 ctgcactgca cctgagagag gtacttcgcg cggttctctg gggcaaccag gctgagggca
      421 gtctgcttga tgttatggaa cgcgcggaag tactcctcgg cagtggtgta gtagtaggag
      481 gattcgttgc catcggtgat gcgggtgccg gggttggcaa catccagcat gccgttgagg
      541 aaggcgggaa gcaacccgta atactcgcgg gcgaggcgac gctcgcgctc agccgggtcc
      601 gacaggctgg cgacactcgg gaagtagctg agctggaaga aggtgtggat cacgggcgcg
      661 gggaggatct cttggatctt cgccatgaac tgtgcgccgc gccgccgggc catcgcctgg
      721 tattccgcga agctcttctc cgcggcgcgt gcctgcgaag gatagtgcca cgggttcttg
      781 ccatagggct ccgcgtcgaa gcaaaggccc ttgcaccggc cgagcttcgc agcctttgcg
      841 cagagcgcga cgttgtggag cacgcactcc cagtcagcat cgctgaacca gtccatgctc
      901 gacgccgcgt acatgatcac gaagttatcc gtgaatttgc gccattcgat gcgctccaat
      961 gccgcgagct ccgcggcaac ctgggactcg tcccacttct tgttggcgaa gacggtgccg
     1021 atgttgggca cgcgcacgat cacgccctcg aacggctgct tttccatctc gcgaatgtgg
     1081 gtcgctacga agctgggcgg tggcacatcc catccatact cgatcaactt cttcgggggc
     1141 gcctcggcgg ccgccggcac gagcgacgca atgatcaggg cagcacacaa tatacgggag
     1201 tacatcgggc ctctcctcct ctggaaaccg aacggtgccc tcttgttcgg cggtgaggag
     1261 gcgcgctcct cttccaggag cgttgggagg catgttgtcg gatccgctcc gggtccgggg
     1321 ttatccggcc acgagaccca gggtcaggag accctgggct cgattgtcgg accgctccgc
     1381 gatccaccgc gagccccacg cgggtagctc gaccacgatc ccgggtggaa cgcgcagccc
     1441 aaggcgcgcc cgcctcaccc atccgtgccc gaccccatat ccgtaatatc ggcgccggtc
     1501 cgcggcctca tccagccggc tcgatcagga cctccatgag aggggagttg acgggcgggt
     1561 gtagggcatc ccccagtggt gccagtgggg ccaccggctt gcggcgcaga ttcggcacca
     1621 ggcggttgcg ggcgatggcg cgctcgtagg cctgtggctt gtcatacaca agaagcacgt
     1681 tcgtcaggcg ccggctcggc ttgctgatga tccgcccgcg gatggagagc gccgtcgagg
     1741 agccgccatc catccccagg gcgttcacgg cccccagcgc cttcatcacc ttgctgacgt
     1801 gggtgtagta gaccggccgg tcgattgtga cgagcaggag cttgttgtac ttcgtcagcc
     1861 cgatggcggc gcgcggtcgg cgggcgtaga gggatgcgtc gcggaagcct tcggcccagg
     1921 ggcgcacggt gcgtaccccg ttctttacca gccagggccc ggcgcagagg accatctcat
     1981 aaggggccca atcctccttg cgtccggccc gcaggggcac gaagtcgatc cggttcccag
     2041 cgaaggcaac gccagttcct accatgccct gatgcacctg ttgccctgcg acgacaatat
     2101 cgcccacggg aaccagggaa cgcgtgtcaa agaaggtgcc cgtgatggcg gcggcggggt
     2161 ggacgcggtt gatgagcgac ttgaagttct cggaggtgcc gatgccgccc ttggcgatgg
     2221 cggcggtgac gcacacatcc gggtcgttga ggttgaccgt gatgacgttg accgggagcc
     2281 ccagaatgac ctttctctcg tagacgacgc tcgagcgatc ggcgcgggct cctggaagca
     2341 accccagcag catgatgcca gcgagacaaa ccgttcttcg cactcccact cccccacact
     2401 ccttcccacc tcttggaggc aagggggata ccaaatgcgc gcctgaacga tgaccgccat
     2461 ggcagggaag atctcccgca tggcgcaaac aggcggttcc cccggctgcc cccgggctgg
     2521 ctcatgcgct tctctggggt tctctgcggg tcgatgcgcg ggcgagagcc cgctcagcgc
     2581 accgcgagtg cctcctcctt gctccggtgc acgctggaga cgccgctcag accggcgatc
     2641 tccaggagcg tgcggatatg gtcattttcg gtgcagattt gaagctgggc cccttcccgc
     2701 aggcgcttgt aggcgcccac gatctgcccg acacccacgc tatccataaa ggagacgccg
     2761 cgcaggtcga taaccagccg gcgcgttgcc ttctccagac cctcatcgag ggcggcacgg
     2821 aacttggggg cgctcgcgat atcgacttcg cccctacact cgaccagaca ggtaccctct
     2881 tcgcactcag ccacccgcgc ctcaaacaga ggaaaccgcg gtcgcgtctt cagcatgctg
     2941 cgccagtcct ctctaagact ctggtcaggg cctgccgggc gggtgcttct gaatgattgc
     3001 ccggtatggg gacgttcgcc cgacccacga tgttcaagcc tgcctgcgcc tttccggatt
     3061 cctatcggca gatctcagcc cttctttacg ccagacagac gaacttcagg ggggaccttt
     3121 gtcctccctg gggtgtgctg ccccttatcc gtgtggccgg cgacaaaaaa agcccggtgg
     3181 tgccgggcag gaagaaagag cacggggcga tgctgtacgt tgtttcggcg agcatatgcc
     3241 acggccgcag ccgcggtgtc acgtcacgcc ctagcaagca cttgcgcgct tgcggctctt
     3301 ctttctgcct acggggttag ctgacgggct cgggcgaaga gtacccgccc tgccgatgca
     3361 gggcacgccc cagtacagtg gttcccccgt tttccctcat ctggaaattc ggccggcgtc
     3421 acacgatcca ctatgaacct gtctgccggg cgcctcacgc ccggtgaaca ctctgcgatt
     3481 ataccacatc catccggtag cgtaaacatc tcaggcaggt aggaagaagg ctcgtcgaga
     3541 ataacgtggc atgcctggca aggagttgct gcccatggaa gaaccgatgc gatatcttag
     3601 cctccttatc tccctcctag cggcgatggc atctgcgtgt atgccggcgc gcgc
//
LOCUS       DAOSCV010000421         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16216_length_3637_cov_2.912898, whole genome shotgun sequence.
ACCESSION   DAOSCV010000421 DAOSCV010000000
VERSION     DAOSCV010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16216_length_3637_cov_2.912898"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="PLY56_10855"
     CDS             <1..1045
                     /locus_tag="PLY56_10855"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ22025.1"
                     /translation="PAKTVTIYKQQDVYACFPSLAQREGEDTLSTGFGTRTRRSHIDP
                     TGGSKRMVSRDGGLTWAESTETFPLSQYRTKDGRLVRASARGWLYVNAEEKDRLVKEQ
                     RIVMDVRPGTVAYLSPDYYAQVSEDGGKTWKMEVIPGPEYVAGLMPFRDESSYVRTTE
                     GVRLVAIYGHRKGVKEREVFILRSADDGKSWQFIPLLPEGPQGVDLNETALVETARGT
                     VLAMARSETDGFLWQSTSTDGGLTWSRPERTELWGHPAHLLRLPDGRILCTYGYRKFP
                     MGIRACVSSDHGRTWPAEKRYVLRADGSGSGGDLGYPITARLSDGTLFTIYYFNGSDN
                     ITHIAGTRWQLPE"
     gene            1177..2817
                     /locus_tag="PLY56_10860"
     CDS             1177..2817
                     /locus_tag="PLY56_10860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ22026.1"
                     /translation="MRTMCGAIVSRLLLLAAVPPLPAQGEDDWQSFYDGSFRLANARA
                     WVSDLTRSAKTAQGLHIVDPSQEKGSARLYFAPWIVDREKGIEAEARLKSVSCSEGWG
                     VVLSLADGMHEEGITFYPDRVELSHARVSAAFNTADAFHTYRVRCQGTDIEVWADGHL
                     LIDGKGKFSAPAHSGRCQIAFGSGSSTAMGEAYWEYVRFRGAKTEPKPKMTVPKVPGL
                     EIVPGEVRTILPDRQYVSMFKRADGALIVGDRCSRDGGQTWERAESFHVGAYQFPDGE
                     IVQLGFATKRSDRPGWFTVPLKRSRDGGKTATTEDALLHLPEATGGTGDDGKYYEGPM
                     VDHAIVGLRDGSLLAVMYGYFKTDTVLCPTFPPEWKFYKYRTWVVRSTDRGRTWEYLA
                     TVAYDPEVGLESFCEADLLALPNGEILCFMRTGGSGGKHTPLYLSRSKDDGKSWSKPE
                     PIADRGVWPNACRMKSGILVCTYGRPGNWLCFSTDEGKTWTGHFCFYDGPTTSYNSVE
                     EVAPDTLLVVFDRQELNDDGNLTRDVVGLTVKVRRLGR"
     gene            3103..>3637
                     /locus_tag="PLY56_10865"
     CDS             3103..>3637
                     /locus_tag="PLY56_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22027.1"
                     /translation="MHRRARGFTLIELLVVIAIIAILAAILFPVFARARENARKANCQ
                     SNLKQIAMACIQYAQDYDERMVRATVTIPTSPGYVTWPYLLSPYVKSTKAFTCPSAPS
                     HAWNGYISVTQTLGYGYNNLLRDSIPLAQITYPAQLAAFADAGRLGPVDSQGRGTGNS
                     YYLMDWDQYQGDNAVPPY"
BASE COUNT          656 a         1105 c         1232 g          644 t
ORIGIN      
        1 tccggccaag acggtgacga tctacaagca gcaagatgtg tatgcctgtt tcccctccct
       61 ggcgcagcgg gaaggcgaag acacgctcag caccgggttt ggaacgcgca cgcggcgcag
      121 ccacatcgat ccgaccggtg ggagcaagcg aatggtctcg cgcgatggcg gcctcacctg
      181 ggccgagagc accgagactt ttccgctttc ccaatatagg acaaaggatg gccggctggt
      241 gcgggccagt gcccgtggct ggctgtacgt gaacgccgag gagaaggatc ggttggtcaa
      301 ggagcagcgc atcgtcatgg atgtgcgccc gggaacggtg gcttacctca gccccgatta
      361 ctacgcccag gtctcggaag atgggggcaa gacgtggaag atggaggtga ttccaggccc
      421 ggagtatgtc gccgggttga tgcctttccg ggatgagagc tcttacgtgc gtaccacgga
      481 gggggtccgc ctcgtggcga tctatggcca ccggaagggc gtgaaggagc gcgaggtttt
      541 catcctgcgt tcggccgatg acggcaagag ctggcagttc atcccgttgc tccccgaagg
      601 gccccaaggg gtggacctga acgagaccgc gttggtagag accgccaggg ggaccgtcct
      661 ggcgatggcg cgctccgaga cggatggctt cctctggcag tccacctcca cggatggggg
      721 cctcacctgg agccggccgg agaggacaga gctctggggc cacccggcgc acctcctccg
      781 gctcccggat ggtaggatcc tgtgcaccta cggctaccgg aagttcccga tgggcatccg
      841 ggcctgcgtg agctctgacc acggccggac gtggccggcg gaaaagcggt atgtcctgcg
      901 cgcggatggc agtggcagcg gtggggatct gggctacccg atcactgcgc gcctatccga
      961 tggcaccctt ttcaccatct actacttcaa cgggagcgat aacatcacgc acattgccgg
     1021 cacgcgatgg caactcccgg agtgagtcgg cagccgcgct tcggtcgagt gagcccatct
     1081 tgcccggggc ggcgctgtct tcccgggcgc cgggcgatgc cagccacgag cggcgatggg
     1141 caggccggcc tgccggcatg gaaaggcacg agagtgatgc ggacaatgtg tggtgcgatc
     1201 gtttcacggc tcctgctcct ggcggcggta cccccgctcc cagcgcaagg cgaggacgac
     1261 tggcagagct tctacgatgg cagcttcagg ctcgcgaacg ccagagcctg ggtgtccgat
     1321 ctgacgcgct cggcgaagac cgcgcagggc ctccatatcg tggatcccag ccaggagaag
     1381 gggagtgccc gcctctactt cgccccctgg atagtcgatc gtgagaaggg catcgaggcg
     1441 gaggcacgcc tcaagagcgt ctcttgcagc gaggggtggg gcgttgttct ctcgctggca
     1501 gatggcatgc atgaagaggg catcactttc taccccgacc gggtggagct gtcgcacgcg
     1561 cgagtgagcg cggcgttcaa taccgcggat gccttccaca cctatcgcgt gcgctgccag
     1621 ggcaccgata tcgaggtgtg ggcagatggg cacctcctga tcgatgggaa gggcaagttt
     1681 tcggcaccgg cgcacagcgg gcgctgccag atcgccttcg gatccgggag ctcgacggcg
     1741 atgggggagg cgtattggga gtacgtgcgc ttccgcggcg ccaaaaccga gccgaaaccg
     1801 aagatgaccg ttcccaaggt gcccgggctc gagatcgtgc cgggcgaggt gcgaacgatt
     1861 ctgcccgaca ggcagtacgt cagcatgttc aagcgcgcgg atggggcgct catcgtcggt
     1921 gaccggtgtt cgcgagatgg tggacagaca tgggagcgtg cggaaagctt ccacgtgggc
     1981 gcctatcagt tcccggatgg cgagatcgtg cagctcggct tcgccacgaa gcggagcgac
     2041 cggccgggct ggttcaccgt tccactcaag cggtcgcgcg atggcggtaa gacagccacc
     2101 acggaggatg ctctcctcca tctccccgag gccaccggag gcacgggtga cgacggcaag
     2161 tactatgagg ggccgatggt agaccacgcc atcgtcggct tgcgcgacgg cagcctcctg
     2221 gctgtcatgt acggctactt caagacagac acggtgctct gccccacctt ccctcctgag
     2281 tggaagtttt acaagtaccg gacgtgggtg gttcggtcta cggaccgtgg ccggacctgg
     2341 gagtatctcg cgaccgtagc ctacgacccg gaagtgggcc tggagagctt ctgcgaggcc
     2401 gacctgctgg cgctgccgaa tggcgagatc ctgtgcttca tgcgcaccgg cggaagcggc
     2461 gggaagcata cgccactcta tctcagccgg tccaaagatg acggcaagag ctggagcaag
     2521 cccgagccga ttgccgaccg gggcgtgtgg ccgaacgcct gccgcatgaa gagcgggatc
     2581 cttgtctgca cctatggacg ccctggcaac tggctctgct tcagcaccga tgagggcaag
     2641 acgtggacag gccacttctg cttctacgac gggcccacta cgagctacaa ctccgtggaa
     2701 gaggtggcgc cggacaccct cctcgtcgtc ttcgaccgtc aggagctgaa tgacgatggc
     2761 aatctgacgc gcgacgtggt gggtctcacc gtgaaagtgc gccggctggg ccgctagcca
     2821 ggagtcttta tcgaggttcg gggtccgggg gctctcgcgc cctgcctttg gacgggtggg
     2881 ggagaacccc cggggggtct ctcgtctctt tctggtatcg ttttgtgata taatatgctc
     2941 acgcaaaggc acggctgggc tctgccgaga gcagcgggcg ggcacggcct gacccgccag
     3001 ccggccagtt gcattcccgc gtcatgggtg accgtggcga gttggatgcg cgcccccagg
     3061 ctttgaccgg ggggcctgac cccttatgaa taggaggatg acatgcatcg gcgggctcga
     3121 ggcttcactc tgatcgaatt gctggtcgtc atcgcgatta tcgccatcct ggccgcgatt
     3181 ctcttcccgg ttttcgcgcg ggcgcgtgag aacgcgcgca aggcgaactg ccagagcaac
     3241 ctcaagcaga tagcgatggc gtgcatccag tacgcgcagg actatgacga gcggatggta
     3301 cgcgccaccg taacgattcc cacctctccc ggctacgtca cctggccgta tttgctctcg
     3361 ccctacgtga agagcacgaa agccttcact tgcccctcgg cacctagcca tgcgtggaac
     3421 gggtatatca gcgtgacgca gacactgggc tacggctata ataatctgct gcgtgatagt
     3481 attcccctgg cgcagatcac ctatccggcg cagctcgcgg cgtttgctga cgccggtcgt
     3541 ctgggccccg tcgactcgca aggccgcggc accggtaaca gctactatct aatggactgg
     3601 gaccagtacc agggggacaa cgcggtgccg ccctatc
//
LOCUS       DAOSCV010000422         3633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16236_length_3633_cov_2.493292, whole genome shotgun sequence.
ACCESSION   DAOSCV010000422 DAOSCV010000000
VERSION     DAOSCV010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_3633_cov_2.493292"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PLY56_10870"
     CDS             <1..1311
                     /locus_tag="PLY56_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22028.1"
                     /translation="GATWAAGLGPDRDSGAGKGTMSGKATMPTERIDLEQFRGQRWAF
                     EEAFPSQLEAHCALHEYCRGNSRAAGQFLGFPPSSHAVKDRWDRLGLPCLGNQNRRAL
                     APGAYTGPQLTSEEAWQQIRQDGRHPARRVRLRSEEVLEVAREAIRERTAALPAVSLK
                     TPHRPPGGGDEEDMVALFSDLQVGHRTVSTNSEVLHQRVQCWAEKVLTIVRLHRKLYP
                     IRRLWVFVLGDMVQNELVGRFIDLDSLEAVVGDQYLRASDFLAEGLYMLAPHFDEIKV
                     KLVRGNHGQVQEYAANRSSWDDVVYERARLLLQNQANLHFEIAERFFLVAEVRGWKFL
                     LVHGDQIPSTLSIPLYGITNRAMRWQGSIGNFDYLCLGHFHSPAMMTWNRTEVLMNGC
                     FVSDDEYVTKRLGFASQPSQLVLGVHDKHGVTWRYRLDLREEQQ"
     gene            1308..1640
                     /locus_tag="PLY56_10875"
     CDS             1308..1640
                     /locus_tag="PLY56_10875"
                     /inference="COORDINATES: protein motif:HMM:NF020715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1937 family protein"
                     /protein_id="HOJ22029.1"
                     /translation="MIVYIAGPYTAPTAGQVDANIGLARDAAAEVYRHGHIPLCPHAL
                     TARFERDYPDIPGPRYLESDLELLARCDAILMLPRWEESRGAQQERERAMSLGLPVYY
                     SVEELPKP"
     gene            1694..2035
                     /locus_tag="PLY56_10880"
     CDS             1694..2035
                     /locus_tag="PLY56_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22030.1"
                     /translation="MPVELCRLMLDRMRGAARKVGHLPPCASGKVSGPGLHEACRWFH
                     AYEACADLDALEMRLEEYLDAFPYCVLEILGEQERRLVPPPPELTNELELIAYLLNRV
                     AARAGLIADKR"
     gene            complement(2101..>3633)
                     /locus_tag="PLY56_10885"
     CDS             complement(2101..>3633)
                     /locus_tag="PLY56_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ22031.1"
                     /translation="RAVSAPPQRPAEYRLAPPVRQAWSPVCPCFPSHRWHRRDHPGPP
                     HNGGAGSSSCYQVCAGNGGYAFASVQLRAVGTRRARAGEFSTVYVWKLDRLSRSVKGF
                     ADLMEEFAAAGTGFASVTENVDYSGSSGRLMMHMLAAVAAFFADLNKERVRAALAHRT
                     QAGLHHGKPPLGYRRSTKGGPLEEDPATAELVREAFRRYAAGDSLAQITQDFNRRGVL
                     RVQGGAWEYTKLKRLLSRRTYLGEVRQGDQWLPGQHPALVNKRTWNRVQERLAANRAI
                     PARSRSNSLSPLLACGYCGGRIDRGSSGGHHRRYYCRACEVHSVSHPPLGAGAAKVEA
                     AIWAYVRWLWEHTDADAELEALVAKRAREKQDGEGAKLRRRLAELDRELAAYLKGFAK
                     GTVPEDVFLATTAPLREERDALAARLNALPRPPDTESLLRDLLAQDAVQTIAKLRTRP
                     ADVQVQWLRRLFARVVIEPGALVFYHAEDLLPPWRMALPPLYQPRRGIGVFVMPDGSC
                     AR"
BASE COUNT          646 a         1177 c         1171 g          639 t
ORIGIN      
        1 ggtgcaacct gggcggctgg acttgggcct gaccgggact ccggtgctgg taagggaacc
       61 atgagcggaa aggcgaccat gccaactgag agaattgatc tggagcagtt tcgcggccag
      121 aggtgggcct tcgaagaagc ctttccatct cagcttgagg cccactgtgc tctgcacgag
      181 tactgccggg gaaacagccg ggcagcaggg cagttcctgg gattcccgcc cagctcccac
      241 gcggtaaagg atcgctggga ccgcttgggc ctcccctgcc tcggcaacca gaaccggcgt
      301 gctctagccc ctggtgccta cactggcccc cagctcacct ccgaggaggc gtggcagcag
      361 atcaggcagg acggcaggca tcctgcccgc cgagtccgcc tgcgctcgga ggaggtgtta
      421 gaagttgccc gagaggcgat ccgagaacgc acagcagctc tgcctgcggt gagcctcaag
      481 acccctcacc ggccccccgg tgggggagat gaggaggaca tggtggcctt gttctcggat
      541 ttgcaagtcg gccatcgcac agtctccacc aactctgagg tgctccacca gcgcgtgcag
      601 tgctgggccg agaaggtcct caccatcgtc cggctacacc gcaagctgta ccccatccgc
      661 cggctgtggg tctttgtgtt gggggacatg gtgcagaatg agctcgtggg gcgcttcatt
      721 gacctcgact cgctagaggc cgtggtcggt gaccagtacc tacgtgcctc ggacttccta
      781 gcagaaggcc tgtacatgct cgcgccccac ttcgacgaga tcaaagtcaa gctggtgcgc
      841 ggcaaccacg gacaggtcca ggagtacgcg gccaatcggt cctcctggga cgacgtggta
      901 tatgagcgcg ctcgcctgct actccagaac caggcgaatc tccatttcga gatcgccgag
      961 cggttctttc tggtggctga ggtgcggggc tggaaattcc tcctcgtcca cggagaccaa
     1021 atcccctcca ctctgagcat ccccctctat ggaatcacga atcgagccat gcgctggcaa
     1081 gggtcgatag ggaacttcga ctatctctgc ttgggccact tccactctcc agccatgatg
     1141 acttggaacc gcacggaggt cctaatgaac ggatgcttcg ttagcgacga cgagtacgtg
     1201 accaagcgcc tggggttcgc ctcgcagccg agtcaactcg tgctcggtgt gcatgataag
     1261 cacggtgtta cctggcgcta ccgactcgat ctgcgggagg agcagcagtg attgtctaca
     1321 tcgcgggccc ctacactgcg cccactgcgg gacaggtgga tgccaacata ggccttgccc
     1381 gcgacgcggc ggcagaggtc taccggcacg gtcatatccc cctctgcccg catgccctca
     1441 ccgcccgttt cgagcgggac taccccgaca tccctggccc tcgctacctc gagagtgatc
     1501 tggagcttct cgctcgatgc gatgccatcc tcatgctgcc gcgctgggag gagagccgag
     1561 gggcccagca ggagagggag cgggcaatgt cgctcggcct ccccgtctac tactcggtgg
     1621 aggaattgcc caagccatga ttactcagcg tctgtgggag gcctccacct gggcttgggc
     1681 acggcgactc ggcttgccgg tagaactgtg ccgactgatg ctcgaccgga tgcggggggc
     1741 ggcgcgcaaa gtcggccatc tgccgccctg cgcctccggg aaggtgagcg ggcctgggct
     1801 acatgaggcc tgtcgctggt tccacgccta tgaggcctgt gccgatctgg acgccctgga
     1861 gatgcggctg gaggaatacc tcgacgcctt cccgtactgc gtgctcgaaa tccttggcga
     1921 gcaggagcga cggctggtgc cgcccccgcc ggagctaaca aacgagctgg agctgatcgc
     1981 gtatctgctg aatcgggtcg ccgccagagc tggcctgata gccgataaga ggtaagccac
     2041 cggcatcggg gctggccgcc accatgctct ttagcaggct ttctcctcgg tcaaagccgg
     2101 ctagcgtgca catgaaccgt cgggcataac gaaaacgccg atccctcgcc gcggctggta
     2161 cagcggcggc agcgccatcc tccacggcgg aagcagatcc tcggcgtggt agaacaccag
     2221 cgcccctggc tcgatgacga cccgcgcgaa tagccgccgg agccattgta cttgcacatc
     2281 tgctggcctt gtccgcagct tggcgatagt ttgcacagca tcctgtgcaa gcagatcgcg
     2341 tagcaggctc tccgtgtccg gcggccgcgg gagcgcattg agtctcgcgg ccaaggcatc
     2401 gcgctcctcg cgcaggggag cggtggtcgc cagaaacacg tcctcgggca cggtgccctt
     2461 ggcaaagccc ttcaggtaag ccgctagctc tcggtcaagc tccgccaacc gccgtcgcag
     2521 cttcgcccct tcaccgtcct gcttctcccg cgctcgcttc gcaaccaagg cctccagttc
     2581 agcgtcggca tccgtgtgct cccagagcca tcgcacataa gcccagatcg ccgcctcgac
     2641 cttcgcagcg ccagcgccca gggggggatg ggagacggaa tggacctcac aggcgcgaca
     2701 gtagtagcgc cggtgatgtc ctccgctgct gccccggtcg atgcgcccgc cgcagtagcc
     2761 gcaggccaga agcggcgaga gggagttgct gcgggaccgg gcggggatcg ccctgttcgc
     2821 agcgagacgt tcttgcactc ggttccaggt gcgcttgttg acgagggccg ggtgctgacc
     2881 gggcagccac tgatcccctt gccgcacctc gcccaagtag gtgcggcgtg agagtagccg
     2941 cttgagcttt gtatattccc atgcgccgcc ctgcacccgc agcacgcccc gccggttgaa
     3001 gtcctgcgtg atctgggcga gagagtctcc agcggcgtag cggcggaagg cttcgcgcac
     3061 caactcggcg gtggccgggt cctcctcgag tggcccgccc ttggtagagc ggcggtagcc
     3121 gagaggcggc ttaccgtgat gaaggcccgc ctgggtgcga tgggccaacg cggcgcgaac
     3181 tcgttcctta ttgaggtctg caaagaaggc cgccacggcg gctagcatgt gcatcatcag
     3241 ccgcccggag ctgccagagt agtctacgtt ctccgtgacg ctggcaaagc ctgtccctgc
     3301 cgctgcgaac tcctccatca ggtccgcaaa gcccttgacg ctgcgggaga gacgatccag
     3361 cttccagaca tagacggtgg agaactcacc ggcgcgggcg cggcgcgtac cgacggcgcg
     3421 aagctgaacc gaggcgaaag catagccgcc attgcccgcg cagacctggt aacaggatga
     3481 acttcctgcg ccgccgttat gaggaggacc tggatggtct cgcctatgcc atcggtgact
     3541 gggaaagcaa gggcagaccg gagaccatgc ttgccgaact ggcggcgcga gccgatattc
     3601 tgcgggcctc tgcggaggcg ctgaaacggc gcg
//
LOCUS       DAOSCV010000423         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16292_length_3625_cov_3.575910, whole genome shotgun sequence.
ACCESSION   DAOSCV010000423 DAOSCV010000000
VERSION     DAOSCV010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16292_length_3625_cov_3.575910"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1787
                     /locus_tag="PLY56_10890"
     CDS             42..1787
                     /locus_tag="PLY56_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ22032.1"
                     /translation="MAEIAHTPQPGSGGPPAVYRPGAAPMVPEDGIDLRRIAEGVYRR
                     RRTLFISLVIAALASVAGALMLPRTYTARTSVLVQSPGSAEALGGSRSREMGPVTAQV
                     DTLREFIQARNFMEPTLRSLGLIEENESPLAIEERIRAVQANIKARPTGANTFEVTYT
                     GKDPAWPVRIVRGVVDGFIAVSTRSRTEQSEEAIAFLEDQMAEYRQRMAKTREAIEAF
                     QAKNAALLAQIPETTSGRVARIEDELAEAQVSAVDAQMKMALIRKQLENTPRTIVAET
                     STVPNPRVLAFQTRLSTAEAELAALLARYTEEHPEVRSKLREVEGLRAEFAEASKQPT
                     AAGSQTTRINPAYERLKDELLAAENQLQTARAREAQLRSMLTPVRSLAKDVPAVRQQW
                     EYLLAQARTQEALYNSLVERLESARMANQLEARQQEAVYRLIDPARIVPSSNGMRRLA
                     IVLAGLGVGFLIGLMLMGLQEMTDRRVHSVQDLERVVGVPVLAAIPQLPEAPRAILVR
                     QRRRQWLVGTAIATAVIAALFLLGTRTQVGREFVRDRMAPVPNTAPASMNPLPNSGES
                     APATPSRPDRPERGR"
     gene            1798..2520
                     /locus_tag="PLY56_10895"
     CDS             1798..2520
                     /locus_tag="PLY56_10895"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ22033.1"
                     /translation="MKRENLPSVRETFTDHYSLIEQEDFGHRLLEEYRTLYTAAMGRL
                     SREGGIVLGVSSPLPGEGKTTVATNIAGAMAADLEKRVLLTSCAFTSGEPQAQGNQRT
                     HLGLADLIADPDQDLDQLIVRTPLPNLSVLLPGTRPDNPSRLLRSQRMAQALERLRDA
                     FDITVFDMMPMLSASDSQVLAAQMDGVLLVVGLGQTGMDAVQRAVRMLGEGRLIGVAA
                     NRAHPLLPRWMLHFLGGGEWGA"
     gene            2508..2663
                     /locus_tag="PLY56_10900"
     CDS             2508..2663
                     /locus_tag="PLY56_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22034.1"
                     /translation="MGSVAVVLALLGAGILVGRCAGPSLAARYLLSLAAAFAISALFL
                     AWVMLAS"
     gene            2884..>3625
                     /locus_tag="PLY56_10905"
     CDS             2884..>3625
                     /locus_tag="PLY56_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22035.1"
                     /translation="MGFFTNRTQMTGQRATDERRQLVVGADEPRTFQRIRPPSPVDRK
                     EPGLSSRMLEGLLLKTLKQEGAMTEQELSDFLCLAPGCFRVELQSLYQRQFIDSAAPM
                     TFDITAKGRELARHYEAEDAYVGPAPVSFEAYCRMVLHQASQGRRLTQEEFDQAFAHL
                     PAPFEMRRMLKEAFNSGQVLLFWGPPGNGKSLWSDSLHALLKDPVLLPHAFEFNNRVV
                     QYYDPAYHKLLPDLMKQEEDAFRNTPNLA"
BASE COUNT          601 a         1201 c         1232 g          591 t
ORIGIN      
        1 ccggatgcga taggctcggc cctatcgtgg gaggagcttc catggcagag attgctcaca
       61 cccctcagcc ggggtccgga gggcctccgg cagtataccg gcccggcgcg gcaccgatgg
      121 tgcccgagga tgggatcgac ctgcgccgga tcgcggaagg cgtctaccgg agacggcgca
      181 cgctctttat cagtctggtg atcgcggcgc tggcctcggt cgctggggcg ttgatgctcc
      241 ctcgcaccta cacggcgcgc acttcggtgc tcgtgcaatc gccgggctct gccgaggcac
      301 tcggcggatc ccggtcgcgt gagatggggc ccgtgacagc ccaggtcgat acgctgcgcg
      361 agttcatcca ggcccgcaac ttcatggagc cgacactgcg cagcctgggg ctgatcgagg
      421 agaacgagag tcccctcgcg atagaagagc ggatccgcgc ggtgcaggca aacatcaagg
      481 cgcgcccgac cggggcaaac accttcgagg tcacctacac cggcaaggac ccggcgtggc
      541 cggtgcggat cgtgcgcggg gtggtggatg gcttcatcgc cgtcagcacg cgcagccgca
      601 ccgagcagtc cgaggaggcc attgcgttcc tcgaggatca gatggcggag tatcgccagc
      661 gcatggccaa gacgcgcgag gcgatcgaag cattccaggc gaagaacgcg gcgctgctcg
      721 cccagatacc ggagaccact tcggggcgtg tggcgcggat cgaggacgag cttgccgagg
      781 cgcaggtgag cgcggtcgac gcgcagatga agatggcgct catccgcaag cagcttgaga
      841 acaccccgcg gacgatcgtc gcggagacga gcacggttcc caacccgcgc gttctcgctt
      901 tccagacgcg gctctccacg gcggaggcgg agctggcagc gcttctggca cgctatacgg
      961 aggagcaccc ggaggtccgc tccaagcttc gcgaggtgga gggcttgcgt gccgagttcg
     1021 cggaggcaag caagcaaccc accgcggcgg gatcgcagac gacgcgcatc aacccggcct
     1081 acgaacgcct gaaagacgag ctgctggcgg cagagaacca gcttcagacg gcgcgggcac
     1141 gcgaggcgca gctcaggagc atgctcaccc ccgtccgctc gctcgcaaag gatgttccgg
     1201 cggtgcgcca gcagtgggag tacttgctgg cccaggcgcg cacgcaggag gcgctctaca
     1261 attcgctggt cgagcggctt gaaagcgccc ggatggccaa ccagctcgaa gcgcggcagc
     1321 aagaggcggt ctaccggctg atcgacccgg cgcgcatcgt gccctcgtcg aacggcatgc
     1381 gccgcctggc gattgtgctc gcggggctcg gtgtcggctt cctcatcggg ctcatgctga
     1441 tgggcctgca ggagatgacc gaccgccgcg tgcattcggt gcaggatctg gagcgcgtgg
     1501 taggtgttcc ggtgttggcc gccattccgc agctgccgga ggctccgcgc gccattctgg
     1561 tccgccagcg ccggcgccaa tggctcgtgg gaacggccat cgcaaccgcc gtgatcgcgg
     1621 cgctcttcct cctgggaacg cgcacgcagg tgggccgcga gtttgtgcgc gaccgaatgg
     1681 ctccggtgcc taacactgcg ccggcctcaa tgaaccccct ccctaattct ggggagagcg
     1741 cgccggccac cccgagccgt ccggatcgcc ccgagcgtgg caggtaagga gccagggatg
     1801 aagagagaga acctcccgtc tgtgcgggag acatttaccg accactacag cctcatcgag
     1861 caggaggatt tcggacaccg tctgctggag gagtaccgaa ccctctatac ggcggccatg
     1921 ggccggctga gcagggaagg gggcatcgtc ctgggtgtct ccagcccgct ccccggcgag
     1981 gggaagacca ccgtggccac caacatcgcg ggcgccatgg cggccgatct ggaaaagcgc
     2041 gtgctgttga caagctgcgc gtttacctcg ggcgagccgc aggcgcaggg gaatcagcgg
     2101 acgcacctcg gtctggccga cctcatcgcg gatccggacc aggatctgga ccaactgatc
     2161 gtgcgcacgc ccttgcccaa tctgagcgtg ctgttgcccg ggacacggcc ggataacccc
     2221 agccgcctcc tgcgtagcca gcgcatggcg caggcgctgg agcggctgcg cgacgccttc
     2281 gacatcacgg tcttcgacat gatgccgatg ctctcggcga gcgactcgca ggtgctcgcc
     2341 gctcagatgg atggcgtgct cctcgtcgtt ggcctgggtc agaccgggat ggatgccgtc
     2401 caacgcgcgg tgcggatgct gggcgagggc cggctcatcg gggtggcagc gaaccgtgcc
     2461 catccgctgc ttcctcgctg gatgttgcac ttcctcggag ggggcgagtg gggagcgtag
     2521 cggtagttct ggcattgctc ggggccggca tcctcgtggg caggtgcgcc ggcccatccc
     2581 tggctgcgcg ctatctgctc tccctggcgg cagcatttgc catcagcgcc ctcttcctgg
     2641 cctgggtgat gcttgcaagc tgaccgagaa ggggcagggg acacgatccg gttcgctgcc
     2701 cctcgcttgg gtgatgccgg gttctgaggg aagactccat tctccccccg cacgcggccg
     2761 gatcgcgcat ccgctctccc agcccgcggt gtcctgcagg atgctctttg gcctgcggcg
     2821 aactcttgcc cagggccggc cgcgcgagaa gcgtgccagg cagccgcttg caggagggga
     2881 tcgatggggt ttttcaccaa ccgcactcag atgacggggc agcgcgccac ggatgagcgt
     2941 cgccagctag tcgtgggcgc cgatgagccg cgcacgttcc agcggatccg tcccccctcg
     3001 ccggtcgacc gcaaagagcc gggcctctct tcgcgcatgc tcgagggcct cctcctgaag
     3061 acgctcaagc aggagggcgc gatgactgag caggagctaa gcgacttcct ctgcctggcg
     3121 ccaggctgct tccgcgtgga gttgcagtcg ctctaccagc gccagttcat cgactccgcc
     3181 gcgccgatga cgttcgatat caccgcgaag ggccgcgagt tggcgcgcca ctacgaggcg
     3241 gaggatgcct acgtcggccc ggcgcccgtc agcttcgagg cctactgccg catggtgctc
     3301 caccaggctt cccagggccg gcgcctcacc caggaggagt tcgaccaagc attcgcccat
     3361 ctgcctgctc cgttcgagat gcggcgcatg ctcaaagagg cgttcaactc cggccaggtg
     3421 ctcctcttct ggggccctcc gggcaatggc aaatcgctct ggagcgactc gctgcatgcc
     3481 ctccttaagg atccggttct gctcccccac gccttcgagt tcaacaaccg cgtcgttcag
     3541 tactatgatc ccgcgtatca caagctgctt ccggacctga tgaaacagga ggaggatgcc
     3601 tttcgaaaca cgcccaacct ggctg
//
LOCUS       DAOSCV010000424         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16304_length_3623_cov_2.953756, whole genome shotgun sequence.
ACCESSION   DAOSCV010000424 DAOSCV010000000
VERSION     DAOSCV010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16304_length_3623_cov_2.953756"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLY56_10910"
     CDS             complement(<1..376)
                     /locus_tag="PLY56_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ22036.1"
                     /translation="MKSAKSAAARLSVLSNTALVTMKVIVGMLTGSVSILAEALHSGL
                     DLVAAGIAWVAVRAADAPPDEEHPYGHGKVENLSGTVEAALILFTAGWIAWEALQRLV
                     DPRPVHASGLGLVVMAVSSGVNV"
     regulatory      complement(457..542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            complement(715..1392)
                     /gene="nth"
                     /locus_tag="PLY56_10915"
     CDS             complement(715..1392)
                     /gene="nth"
                     /locus_tag="PLY56_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ22037.1"
                     /translation="MTSHLRSSSHPHDAALLQDASSRAEEIIRRLHQEYPEAHCALGH
                     SSALELLVATILSAQCTDARVNQITPGLFRKYPTIEDFAAADQEELERDIFTAGFYRN
                     KAKAIIGMARAVLERHGGEIPNTMEELVALPGVGRKTASVVLGSWFGVPALPVDTHVT
                     RLARRLGLTESDDPNRIERDLQALLPEEEWVFASHALIWHGRRVCFARKPECDRCCLR
                     DLCPSAA"
     gene            complement(1429..2424)
                     /locus_tag="PLY56_10920"
     CDS             complement(1429..2424)
                     /locus_tag="PLY56_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009144994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOJ22038.1"
                     /translation="MTPPQENTQSIQQLAAEVLEIEARAILKLRERLDESFTRAVKLI
                     LACTGRVVVTGIGKSGAIAHKIAATLASTGTPSLFLHPAEGVHGDLGMATRGDLVIVL
                     SHSGETEEIVRILPAFKRLGIPIIALCGNPHSTLGEQSEVFLDTSVEREACPLGLAPT
                     TSTTVMLAVGDALALCAMKERRFTRDDYARFHPGGSLGRRLLLRVSDLMRTGDLVASV
                     QGDTTILDTLLAITQARAGCAIIVDEEQHLEGIITDGDVRRLLLDDPDCLHRKVAGVM
                     NRSPATITVDRLAAEALQVMESHRPGAIGDLPVLDDEGRLVGVVTLKDLLKAGIV"
     gene            complement(2539..>3623)
                     /locus_tag="PLY56_10925"
     CDS             complement(2539..>3623)
                     /locus_tag="PLY56_10925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22039.1"
                     /translation="LVVEEDGRFVSACTCLPAPVWVGDRRLTLGAVGGVATLPDYRRR
                     GYAGELMRAVVRRMQELGLATSALWPFSYAYYRKFGWDFGCEHRTYRFDPTKIGPLPE
                     PDGVEPLQAGDLPEVVDLFSAYARTLAFCTDRSQRWWQELLRNKGVAPFAPEEKATSR
                     MIIYREGGEVVGYALYTPPAGEERRLNVAELVASGPEARMRLLAALAREEPAEITFRA
                     PADDRFRAQVPDPRQVATMLEPGFAFRIVDPVVALALLPPPPGLRGAIAFTLDDPARP
                     DEPLRVTVEVSDGELVTSASRTMHSAAPMRLHAEIASFSQIYTGYLRPTVAHHLGRLK
                     GDAASVAFAEALFPHWTAYRSAVEPG"
BASE COUNT          653 a         1210 c         1161 g          599 t
ORIGIN      
        1 acacgttcac ccccgaggaa accgccatta ccaccaagcc aaggccgctc gcatgaaccg
       61 gccggggatc caccaaacgc tggagcgcct cccaagcaat ccatcccgca gtgaagagga
      121 tgagcgccgc ttctaccgta cccgacaggt tctccacctt gccgtgacca taggggtgtt
      181 cctcatccgg tggagcgtcc gccgcgcgca cggcgaccca ggcaatcccg gccgccacca
      241 gatccagccc agagtggagc gcctccgcga ggatgctgac cgagccggtc agcatcccga
      301 cgatcacctt catcgtcacc agcgccgtgt tggagagtac cgatagccgc gctgcggcgc
      361 tctttgcgct cttcatcccg cctcccactt caagcaccag cgttctgctt ccgtttcctc
      421 ttagtacaaa caaaagcagg acgacccaag cggagatgta gtcccgcagg tcatcctgct
      481 gcttcgatca ctgaagcccg gccccggtgg catacgccac ccgcctgctc tactcaacca
      541 accccatcct accacacgat ttccagcgcg tcaatgagag agcgccgcga taccacgatg
      601 aagggggcag gcatggatga gccgtgtgcg gacacccggg gttgtgcgtc cgtccgggtt
      661 tgaggggacc gccgcacgcc ggtcgcaggg gatccgggtt gagagagggc cgggctaggc
      721 ggcggatgga cagaggtcac gcaggcagca gcgatcgcat tccggtttgc gcgcgaagca
      781 gacgcgccgg ccatgccaga tcaaggcgtg ggaggcgaac acccactctt cctcggggag
      841 caacgcctgt aggtctcgct cgatccggtt ggggtcgtcg ctctcagtca gacccaggcg
      901 cctcgccagg cgggtgacat gggtatctac tggaagggcc ggcacgccga accaggagcc
      961 caggaccacg ctcgcggtct tccgtccgac accgggcagc gccacgagct cttccatcgt
     1021 attcgggatc tcgccgccgt ggcgctccag caccgcccgc gccatcccga tgatcgcctt
     1081 cgccttgttt cgatagaagc ccgccgtgaa gatgtcgcgc tccagctcct cctggtccgc
     1141 cgcggcgaag tcttcgatgg tggggtactt ccgaaacagc cccggggtaa tctggttgac
     1201 ccgcgcgtcg gtacactgcg ccgagaggat cgtcgcgacg agcagctcca gggcactgga
     1261 gtgccccagc gcacagtgcg cctctgggta ctcctgatgc agcctgcgga tgatctcctc
     1321 cgcgcgagag gaggcatctt ggagcagggc cgcgtcatgc gggtgcgagg aggaacgcag
     1381 gtgtgaagtc atcgcggttt cgcttgccca ccgtgtgaaa cggcgtcctc agacgatccc
     1441 tgccttgagc agatccttca gggtgacgac tccgaccagg cggccttcgt catccagcac
     1501 ggggagatcg ccgattgccc cgggccggtg cgattccatc acctggagag cctcggctgc
     1561 aagacggtca acggtgattg tcgctggcga ccggttcatc acgcccgcca ccttgcgatg
     1621 caggcaatct ggatcgtcca gcagcagccg gcgcacgtcg ccatcggtga taatcccctc
     1681 cagatgctgc tcctcgtcca cgatgatggc gcatcccgcg cgcgcctggg tgatcgccag
     1741 gagcgtatcc aggatcgtcg tatcgccctg aacgctcgcg accagatcgc cggtgcgcat
     1801 cagatcggag acgcgcagga gcaggcgccg gccgagggag cctcctgggt ggaagcgcgc
     1861 atagtcatcg cgcgtgaagc gacgctcctt catcgcgcag agcgccaggg catcgcccac
     1921 agcgagcatc accgtcgtgc ttgtggtggg cgccaggccc agcgggcacg cttcgcgctc
     1981 gaccgaggta tcgaggaaga cctccgattg ctcgccgagc gtggagtgag gattgccgca
     2041 gagcgcgatg atcggaattc ccagccgctt gaaggcaggc aggatgcgca caatctcctc
     2101 ggtctcgccg ctgtgagaga ggacgatcac cagatcgccc cgggtcgcca tgccgagatc
     2161 gccatgcacg ccttccgccg gatgcaggaa aagcgagggc gtgccagtgc tggcgagggt
     2221 ggcagcaatc ttgtgggcga ttgctccgga cttgcctatc cccgtcacaa ccacgcgtcc
     2281 ggtgcaggcg agaatgagtt tcacggctcg cgtgaagctc tcatccaggc gctcgcgcaa
     2341 cttcagaatg gcacgcgctt caatctcaag gacctcggcg gccagttgtt gtatcgattg
     2401 agtattctct tgtggtggag tcatcgccgg ttctgttcgc ctttcgcgag gcaattcctg
     2461 cttgccgcgg tcacttcggc gattgcgccg cagatcaggc aggctgccca tcccactggt
     2521 aaggtgatac tctcccgttt atcccggctc gacggcagag cgataggcgg tccagtgggg
     2581 gaacagcgcc tccgcgaacg caacactggc ggcgtctccc ttcagccgtc cgagatggtg
     2641 tgcgacggtt gggcgcaggt atccagtata gatctgcgag aaggaggcga tctccgcgtg
     2701 cagacgcatc ggcgcggcgg agtgcatcgt gcggctggca ctggtcacca gttcgccgtc
     2761 ggatacttct acggtcacac gcagcggctc gtccgggcgt gccgggtcat caagggtgaa
     2821 cgcgatggca ccgcgcagcc cgggcggggg cggcaggagc gccagggcca ccaccggatc
     2881 cacgatacgg aaggcgaaac ctggctcgag catcgtcgcc acctgacgcg ggtccggcac
     2941 ctgggcgcgg aagcggtcat cggcgggtgc cctgaaggtg atctctgcag gctcctcgcg
     3001 cgcgagcgcc gcgagcaggc gcatccgcgc ttccgggccg gatgccacca gctcggcaac
     3061 gttcagccgg cgctcctcgc cggccggcgg tgtgtagagc gcgtagccca ccacctcgcc
     3121 cccttcacgg tagatgatca tccgtgaggt cgccttctcc tctggagcga atggcgcaac
     3181 gcccttgttc ctcagcaact cctgccacca gcgctgagag cgatcggtgc agaacgccag
     3241 ggtacgtgca taggcggaaa aaagatcgac cacctcgggc aggtctccgg cttgaagggg
     3301 ctccacccca tccggctccg ggagcgggcc gatcttcgtg ggatcgaagc ggtaggtgcg
     3361 atgctcgcat ccgaagtccc agccaaactt ccgataatag gcgtaggaga agggccacag
     3421 cgccgaagtg gcgaggccca gctcctgcat gcggcggacg acggcacgca tcaactcgcc
     3481 cgcgtagccc cgcctccggt aatcgggcag cgtggcaacg ccaccgacgg cacctagagt
     3541 gagcctccgg tcacccaccc acacgggagc cggcaggcat gtgcacgccg agacgaagcg
     3601 gccatcttcc tcgacgacga gga
//
LOCUS       DAOSCV010000425         3622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16309_length_3622_cov_3.409027, whole genome shotgun sequence.
ACCESSION   DAOSCV010000425 DAOSCV010000000
VERSION     DAOSCV010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16309_length_3622_cov_3.409027"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PLY56_10930"
     CDS             <1..1558
                     /locus_tag="PLY56_10930"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ22040.1"
                     /translation="SSAYARVIEGALGLGVAAMVRGLPQEERVIQPKAFLLWSVGALS
                     PWAWIHHLALTLFYAVFGFRVFCRVVEHHEALEGNLHRMQRQREVIVGFLDRIGAAYS
                     DALDLEQLLRLLASAAVETTGAGAGAIFLVDSRTGKLQMRAVEGPFPPLYRDVPASNL
                     MRRWEELTRIALRQRFAFGQGVVGEVAAKQTPLRIRDAAEAGILVGSVVDPVRRHPML
                     LTPLVLRGEVLGVMAVLNRQNADEFSEDDQFLLATLAEQAAFYIDNARMVAVLAQQER
                     VRRELQIAREIQRTLLPADAPKVPGFDLDALSRPALEMGGDYYDFFWVAEGHLGIVVA
                     DVSGKGVPAALTMAMLRTVMRTYAFGNRSVHDTLMRANATLCGDLRLDTFITLFYGIL
                     DVKRRTLSWARAGHEPTLLLRDNRIEVLTPSGAAVGVLSPAEFGHSLQVEETVIGPGD
                     AILIYTDGITEAMNHHSEEFGLGRFVETVRGTAGLSSAQQIQRIEESVSAFAGDAPQQ
                     DDITLVVLTG"
     gene            1835..>2882
                     /locus_tag="PLY56_10935"
     CDS             1835..>2882
                     /locus_tag="PLY56_10935"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ22041.1"
                     /translation="MATRSKDRVVVGVVGCGTVGTGAVQVLLSNAAWIAGRVGAPVVL
                     KSVADIDWTRPRGFEVPEALRRSDAESVIADPEIDVVVEAIGGVGMAAQVVRAALARG
                     KSVVTPNKELMAKQGGELLALASEKGVDLMFEGAVGGGIPIIKPMKESLAGDTIERIV
                     GIVNGTTNYILTGMARENRDFADALASAQALGYAEADPTADVEGLDSLYKIAILASIA
                     FGSRVSVSDLYHEGITRVTAADIGYARQLGFAVKLLAIAARLGGRVDIRVHPAFIPLE
                     HPLASISDVFNGVLVRGRQVGDVMFYGRGAGQGPTGVAVVGDVVDCARNLLHGAAGRV
                     PCRCGCSLPLLPMEE"
     assembly_gap    2883..2892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2893..3191
                     /locus_tag="PLY56_10940"
     CDS             <2893..3191
                     /locus_tag="PLY56_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ22042.1"
                     /translation="GQTYAAPRNRVEEVVCRNYVRMRVTDRPGVIGRIATCFGSHGVS
                     LASVFQPDTEVGPGLAEIVWITHAVQERQMRAALAEIATLPMVNEVASVIRLEG"
BASE COUNT          552 a         1143 c         1249 g          668 t
ORIGIN      
        1 gagttcggca tacgcgcgtg tcatcgaggg cgcgctcggt ttgggggtgg cggcgatggt
       61 gaggggcctc ccgcaggagg aacgggtcat tcagcccaag gcgttcctgc tgtggagcgt
      121 gggcgcgctc tcaccgtggg cctggatcca tcatctggca ctcaccctct tctatgcggt
      181 tttcggcttt cgcgtcttct gccgggtggt ggagcaccat gaggcgctcg agggtaacct
      241 ccaccggatg cagcgccagc gcgaggtgat cgttggcttc ctcgaccgca tcggcgcggc
      301 atacagcgac gccctggacc tggagcagct gttacgcctt ctcgcctccg ccgcggtgga
      361 gaccaccgga gcgggcgccg gggcgatctt cctggtcgat tcccggaccg gcaagctgca
      421 gatgcgcgcg gtggaaggcc ccttcccgcc gctctaccgc gatgtgccgg cgtctaacct
      481 gatgcgccgg tgggaggagc tgacgcggat cgcgctccgc cagcgctttg ccttcggcca
      541 gggtgttgtc ggagaggtgg cggccaagca gacgccgctc cgcattcgcg acgccgccga
      601 agcgggaatc ctggtgggca gtgtcgtcga tccggtgagg cgccatccga tgcttctgac
      661 gcccctcgtt ctccgcggcg aggtgcttgg cgtcatggcc gtgctgaacc ggcagaacgc
      721 agacgagttt tcggaggatg accagttcct gctggccacc ctcgcggaac aggcggcgtt
      781 ctatatcgat aacgcgcgca tggtggccgt tttggcgcag caagagcgtg ttcgccgtga
      841 actacagatc gcccgtgaga tccagagaac gctcctccca gccgatgctc ccaaggtgcc
      901 cggcttcgac ctggatgcgc tcagtcgccc cgcgctcgag atgggcggcg actactacga
      961 tttcttctgg gttgccgagg ggcacctggg catcgtggtc gccgatgtct ccgggaaggg
     1021 ggtgcctgcg gcgctcacga tggcgatgct gcgcacggtg atgcgcacgt atgcgttcgg
     1081 gaaccgatcc gtgcacgaca ctctgatgcg cgccaatgcc actctttgcg gcgacctgcg
     1141 cctcgatacc ttcatcacgc tgttctacgg catcctcgac gtgaagcgcc ggacactctc
     1201 ctgggcgcgc gccggccacg agccgacgct gttactccgc gataaccgca tcgaggtgct
     1261 caccccctcg ggcgcggccg tgggagtcct ctcgccggcc gagttcggtc acagcctcca
     1321 ggtagaggag accgtgatcg gccctggcga cgccatcctc atctacaccg acggcatcac
     1381 cgaggcgatg aaccaccact ccgaggagtt cggactaggg cggttcgtcg agaccgtgcg
     1441 cgggacggcc gggctttcga gcgcccagca gatccagcgg atcgaggagt cggtgagcgc
     1501 cttcgccggc gatgcccctc aacaagatga tatcaccctc gtcgtcctca cgggatgaga
     1561 cgcgcgcggc ggtcggcgct tctccgatga tccccccctg tagggatctg accgccaggc
     1621 ggcgaatgag agagaggcac ctggtcgtcg gtgccgcttt atctttcacg gaaagagttc
     1681 ctcgcgcggc tgcgagccgg ctatgtcgcc tcatcgggtg gagaatggag cctgcgagtc
     1741 tcgtgaccgg atcccaccgg ataccgctcc tccacgcgat gagggctctc ccctgtggct
     1801 cgcgccgttc gaagcgcttg ggagcgaggt tgctttggct acgagatcga aagaccgggt
     1861 tgttgttggc gtggttggct gcggcaccgt gggcacgggc gctgtccagg tgctcctgag
     1921 taacgccgcc tggattgccg ggcgcgtggg tgcccccgtg gtgctgaaga gcgttgcgga
     1981 tatcgattgg acccgcccca gggggttcga ggtgccggag gcgttgcggc gcagcgacgc
     2041 ggagagcgtg attgccgatc ccgagatcga tgtggtcgtc gaggcgattg gcggtgttgg
     2101 catggcggcc caggtggttc gcgccgccct tgcgcgtggc aagtcggtgg tcacgcccaa
     2161 caaggagctg atggccaagc aagggggcga gctcctcgcg ctggcgtccg agaagggcgt
     2221 cgatctgatg ttcgagggtg ctgtcggcgg tggaattccc atcatcaagc cgatgaagga
     2281 gtcgctggca ggcgatacca tcgagcggat cgtgggcatc gtcaatggca ccaccaacta
     2341 catcctcacc gggatggcgc gcgagaaccg cgatttcgca gacgcgctgg cctccgcgca
     2401 ggcgctcggc tatgccgaag cggatcctac cgccgatgtg gaaggcctgg actcgctcta
     2461 caagatcgct atcctcgcct ccatagcctt cgggagccgg gtttccgtga gcgatcttta
     2521 ccatgagggc atcacccgtg tgactgccgc ggatattggc tatgcccgcc agctgggctt
     2581 tgccgtgaag ctcctcgcca ttgccgcccg cctgggaggc cgcgtcgata tccgcgtgca
     2641 tccggcgttc attcccctgg agcacccgct ggcaagcatc agcgacgtct tcaacggcgt
     2701 gctggtccgc gggcggcaag tgggcgatgt gatgttctat ggccgcggcg caggccaggg
     2761 accgaccggc gtggccgtgg tgggcgatgt ggtggactgc gcgcgcaatc ttctccacgg
     2821 cgccgccggg cgcgtgccct gtcgttgcgg ttgctcgctc cccctgcttc cgatggaaga
     2881 ggnnnnnnnn nntggggcag acttacgccg cgccccgcaa ccgcgtggaa gaggtggtct
     2941 gccgcaacta cgtgcgcatg cgcgttaccg accggccagg tgtcatcggg agaatcgcca
     3001 cctgtttcgg cagccacggg gtgagcctgg ccagcgtctt ccagccggat accgaggtgg
     3061 gcccaggcct ggcggagatc gtctggatca cgcacgcggt gcaggagcgc cagatgcgcg
     3121 ccgctctggc cgagatcgcc acgctcccta tggtgaacga agtcgcttcc gtgatccgac
     3181 tggaggggta gcgtctctcc catgttgccc cggtattacc ggcggtaatg gcctcagcga
     3241 tcccgcgcga tgcaggacgg gggtgcccca aacggctgtc tgcccaggcg cgccaccccc
     3301 tccctgggtc cggcgaggga tgatgcggca catccccggg ctgccggttg acgtcccaga
     3361 cgggcgctta gtagaatgga tgagggacac gggggctttc gggcgagcac cggtggagag
     3421 cgtgagctcc gcatcgctcg atctgcttgg gaagtaacac cgatggcatc tttcgatcta
     3481 cacgcctggt ttcattcgct cgagccgacc gatcagtggt tgatagagtg gcgcacgcag
     3541 catgacctgt cgattaagga gatcgcggcg cgcagcggtc tctcccaaag cgccgtggcc
     3601 gagcggctgg cgcggcttcg cg
//
LOCUS       DAOSCV010000426         3595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16478_length_3595_cov_2.751977, whole genome shotgun sequence.
ACCESSION   DAOSCV010000426 DAOSCV010000000
VERSION     DAOSCV010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16478_length_3595_cov_2.751977"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..2449
                     /locus_tag="PLY56_10945"
     CDS             125..2449
                     /locus_tag="PLY56_10945"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083463101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ22043.1"
                     /translation="MERLGYRVEQIAAPPLVLPPSEELVSMKIGGMDCADCVRKLERA
                     VSALPGVRAAQVTLATGRMEVRYDPAVASLDRIARTVRETGYEARIEGNPTHEPEAPA
                     FWEKNTRLALTLLSGIALGVALLIGILGGPGVVEGVLYLLAILLGGVYVLRSAMVALR
                     TRLATDINFLLTVAVIGALAIGEWAEAASVVFLFSVAELLESYSMDRTRNAIRELMRL
                     APDQATVRRDGSEIVLPVQEIRVGEVMVVKPGERLAMDGTVVTGSSLVNEAPITGESI
                     PKEKGVGDDVFGGSINGHGSLEVLITREYEDTTLARVIRLVEDAQAQKARSEQFIDRF
                     ARYYTPAIIALAVVIAIVPPLAFGAAFDTWFYRALALLIIACPCALVISTPVSVVSAI
                     AAAARSGVLVKGGAHLEEIGRARVVAFDKTGTLTRGAPDILEVIPLDGRSEQEVLALA
                     AAIESRSEHVLAEAVLRKAYFEGLRWPELSDFQSITGQGARARVNGEEYYIGRPRLLE
                     QIGVSLDPVRDRIAEEQALGRTALLLGTKERLVGMILAADQPRLAARDTIRELKRAGI
                     ERTVMLTGDNVGTAAAVARELGIDEYHAELLPQQKVQAVHDLIARYGHVAMVGDGVND
                     APALAAASVGVAMGAAGTDVALETADVALMADDLSRFPFIVRLSRQTVRIIRQNIFFS
                     IAVKALFVAITFAGVITLWLAVLADMGTSLIVTANGLRLLRGTHRAAVSPGRVAGGEH
                     AHAHAECRGACPLPIPVADLSGEAGHVEGTHRHE"
     gene            complement(2466..>3595)
                     /locus_tag="PLY56_10950"
     CDS             complement(2466..>3595)
                     /locus_tag="PLY56_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOJ22044.1"
                     /translation="ADYYDNGVFREAHVKLLDRLGFGVLRMAYQAPSSAAPLYRVAAK
                     ALQPERINVPMLLISGWYDPGVASHLRAFEMLRAQAGETTRQHTKMLVGPWHHTAIGQ
                     VRQGALEFPGATGESERVARLFLDYWLRGERQNGWEELAAFRWWQMGEEGWHEATAPG
                     ALATTDWVLTLHADGRLDECPAGAAEPSRAYIADPANPVPTRGGANVGYGTAGGMGIG
                     PLDQSALESRPDVLVYTTPPMEKPRRLFGSAVLQFSFTIDRPDATFAVRLCNVHPDGR
                     SLLICDGATRARYRDGSGRPLQVVPGEVYTAVLTLPPTAITLPPGHRLRISIAGSNHP
                     RFELNPHTGADHFDRATAVSARCTLFHDAARPATIRVPLLP"
BASE COUNT          568 a         1141 c         1247 g          639 t
ORIGIN      
        1 gaagattcgc acagcagtgt cgcgagtttc gggcgtctct gacgtcgacg tcaattttca
       61 gactgagact ctgacattta gtcacgaaga cgctcaaatg gtcagaaagg gtattggaga
      121 gaccatggaa cgcctggggt atcgcgtcga acagattgcc gcgccgccgc tcgttttgcc
      181 gccgagcgaa gagcttgtct ccatgaagat cggcggcatg gattgcgccg attgcgtgcg
      241 gaagctggag cgcgcggtat cggcgctccc gggagtccgc gccgcgcaag tcaccctcgc
      301 gacgggacgc atggaggtgc gttacgatcc ggcggtggcc tcgctcgacc gaatcgcgcg
      361 gacggtgcga gagaccggct acgaggcgcg catcgaggga aacccaacgc atgagcccga
      421 ggcgcccgcg ttttgggaga agaatacgcg cctagcgctc actcttctct ccgggatcgc
      481 cctgggtgtc gcgctgctca ttggcattct cggagggccg ggcgtcgtcg agggcgtcct
      541 ctatctgctg gcgatccttc tggggggcgt ttacgtgctt cgcagcgcga tggtggctct
      601 gcgaacgcgc cttgccacgg atatcaactt cctgctcact gtcgccgtca tcggcgcgct
      661 ggcgatcggg gagtgggccg aggcggcctc ggtggtcttc ctcttttcgg tggccgagct
      721 cctggagtca tacagcatgg atcggacgcg caacgcgatc cgcgagttga tgcgtctggc
      781 gccggatcag gccaccgtcc gccgcgacgg tagcgagatc gtcctccccg tacaagagat
      841 ccgggttggc gaggtgatgg tggtcaagcc gggcgagcgt ctcgccatgg atggcacggt
      901 cgtcaccggt tccagtctgg tgaacgaggc gccgatcacc ggggaatcaa tcccgaagga
      961 gaagggcgtc ggtgacgatg tcttcggggg gagcatcaac gggcacgggt cgctggaagt
     1021 gctgatcacg cgcgagtatg aggacacaac gctggcgcgc gtcatccgcc tggtggagga
     1081 tgcccaggca cagaaggcgc gctccgagca gttcatcgat cgcttcgcgc gctactacac
     1141 gccggccatt atcgccctgg ccgtggtgat cgccatcgtg ccgccgcttg ctttcggtgc
     1201 tgccttcgac acctggttct accgtgctct cgcgttgctg atcatcgcgt gtccttgcgc
     1261 gctcgtcatc tccacgccgg tctccgtggt ctctgcgatc gcggcggcgg cgcgcagcgg
     1321 cgtgctcgtc aaggggggcg ctcacctgga ggagattggc cgggcgcggg tggtcgcctt
     1381 cgacaagacc gggaccctca ctcgtggcgc tccagacatc ctggaggtga tcccgctcga
     1441 tgggagatcc gagcaggaag tgttggcact ggcagcggcc attgagtccc gatccgagca
     1501 cgtgctggcc gaggcggtgc tccggaaagc ctacttcgag ggcctgcgct ggcccgaact
     1561 gagcgatttc caatcgatca ctgggcaggg ggcgcgcgcc cgtgtcaatg gcgaggagta
     1621 ctacatcggc cggccgcgcc tcctggagca gattggcgtt tcgctggacc cagtgcgcga
     1681 ccgcatcgcc gaggagcagg cgctggggcg taccgcgctc ctgctgggca ccaaagagcg
     1741 cctggtgggg atgattcttg ccgccgacca gcctcgcctc gccgcgcggg atacgatccg
     1801 cgagctaaag cgcgcgggca tcgagcgcac ggtgatgctg acgggcgata atgtcggcac
     1861 ggctgcggcc gtagcacgag agttgggcat cgatgagtac cacgccgagc tcctccccca
     1921 gcagaaggtg caggcggtcc acgacctgat cgcacgctac gggcacgtgg cgatggtggg
     1981 ggatggcgtg aatgatgctc cggcgctcgc cgccgcttcc gtgggcgtgg cgatgggcgc
     2041 cgccggcacc gacgtggcgc ttgagaccgc cgacgtcgcg ctgatggcgg acgatctgtc
     2101 gcgcttcccc ttcatcgtcc ggcttagccg gcagactgtg cgcatcatcc gccagaacat
     2161 cttcttctcc atcgcggtga aagctctctt tgtggcgatc actttcgccg gggtgatcac
     2221 cctatggctg gcggtgctcg ccgatatggg cacctcgctg atcgtcaccg ccaacgggct
     2281 ccgcctcctt cgaggcactc accgcgctgc ggtttcgcct gggcgcgtgg cgggtgggga
     2341 gcatgcccat gcccatgctg aatgccgcgg cgcctgccca ctaccgatcc cggtcgctga
     2401 cctcagcggc gaggcaggcc acgtcgaggg cacgcaccgg cacgagtgag cctcgtagcc
     2461 gccttctatg ggagcaacgg cacgcggatc gtcgcgggac gggcggcatc gtggaagagt
     2521 gtgcaacgcg cagagaccgc cgttgcccgg tcaaagtggt cggcaccggt gtgcgggttc
     2581 agctcgaaac gagggtggtt ggagcccgcg atggaaatgc ggaggcggtg gccaggtggc
     2641 agcgtgattg ccgttggcgg cagcgtgagg accgcggtat agacttcacc gggcaccacc
     2701 tgcaacggcc gcccgcttcc atcgcggtag cgggcgcgcg tggctccatc gcagatcagc
     2761 aacgaccggc catccgggtg aacgttgcag agccggaccg cgaaggtggc gtctgggcgg
     2821 tcgatggtga agctgaattg gagcacggcg gatccgaaga gtcggcgcgg cttctccatg
     2881 ggcggcgtcg tgtagaccag cacgtcgggg cggctttcca gagctgattg atccagcggc
     2941 ccgatcccca tgccgcccgc ggtaccatag ccgacgttcg cgccgccgcg ggttggtacc
     3001 ggattcgcag gatcggcgat gtaggcacgg ctcggctctg ccgcacccgc cgggcactca
     3061 tccagtcggc catcggcgtg aagtgttagc acccagtcgg tggtggccaa ggcgccggga
     3121 gccgtagctt cgtgccatcc ctcctcgccc atctgccacc aacggaacgc cgccaactcc
     3181 tcccagccgt tttgcctctc tccgcgcagc cagtagtcca ggaagagacg ggctactcgc
     3241 tcgctctcgc cagtggcgcc cggaaactcc agcgcgccct gtcgtacctg cccgatggcg
     3301 gtgtggtgcc aggggcccac gagcatcttg gtatgctggc gcgtggtctc ccccgcctga
     3361 gcgcgaagca tctcgaaggc gcgcaggtgt gaggcgactc ccgggtcata ccatcccgaa
     3421 atcaggagca tcggcacgtt gatccgttcc ggctggaggg ccttcgcggc cacccggtag
     3481 agcggcgcgg cgctgcttgg cgcctggtac gccatccgca ggacgccgaa ccccaatcgg
     3541 tcgaggagct tgacatgtgc ctcgcggaag acgccattgt catagtagtc ggcgt
//
LOCUS       DAOSCV010000427         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16557_length_3583_cov_2.805556, whole genome shotgun sequence.
ACCESSION   DAOSCV010000427 DAOSCV010000000
VERSION     DAOSCV010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16557_length_3583_cov_2.805556"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="PLY56_10955"
     CDS             <1..1973
                     /locus_tag="PLY56_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ22045.1"
                     /translation="RNLFGAGAWLADATGRPDAGELPARWRMGKDVGARVAQWVGDAP
                     LPGLPPAGPSPAAAEAEACDVLVVGGGTAGAFAAIAAGREGAKVTLLEASHFLGGIGA
                     GGGIHIYYHGFAGGIQDEVDSRTNALSDLFGANQRLTGFHPDAKQVVLEEMAAEAGVN
                     VIYRSALIEARTSGGRVEVAEVASPTGAILYPAAAFVDGTGDADLAAMVGAEFQLGRE
                     RDGLSHHFSQAYQILDANGSIRLANFDAGYVVATDVTDLTRARRLGLKHLWRDTYTAE
                     NRLLTIAPLLGLRQSRQIVGDYVLTLDDQISGRRFPDVISYAKAHYDNHAFDYENESD
                     EAALWVWLLGAWHDPFASEVPYRCLLPKGIEGLLVACRALSLTHDAHMMFRMQRDLQR
                     VGEVAGVAAAMAVKEGTTPRQVDVRRLQEKLLRSGILREEPVVSEASGTVEGWIAALD
                     APQPKEAVWQLAHAGEEVIEPLREVLKNGSERARFWAAVALAMHGRPEAAPELLRCLI
                     ERADVKPEGYRTVPFWQSVVVLLGRIGDPAAVPEIARLLEEPETTLDGLITAIRALGR
                     IGDPQAVPAIQAMLARSDLPTERPFQVSTGGRSGVSDDARWALELAAAETLAMLGAPN
                     ASLAEKHLSDPRAYVRRYAERVRRMSLVPIGF"
     gene            2202..2278
                     /locus_tag="PLY56_10960"
     tRNA            2202..2278
                     /locus_tag="PLY56_10960"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2236..2238,aa:Pro,seq:ggg)
     gene            2707..3297
                     /locus_tag="PLY56_10965"
     CDS             2707..3297
                     /locus_tag="PLY56_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ22046.1"
                     /translation="MRGHSEQVVEPDLLERVWMDRLRVHDSAAWDELVERFNAPLTHL
                     VCSLLGGWCEDVPDIVQDSFVKAFGSIARFRGECSLRTWLFRIAVNLCRDYQKSAWRR
                     RVDLQGNPSETRLVEPQGVAEQPFRDVEWEQLRLALSRVPEKERVCVELRFIYQMTGR
                     EISAVVGCTESTVWSRIYAGLKRLRAALSEEEMTGK"
     gene            3342..>3583
                     /locus_tag="PLY56_10970"
     CDS             3342..>3583
                     /locus_tag="PLY56_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22047.1"
                     /translation="MIKAVAGCLPERRRRGLERRIARCDACRQDREQLEETAGLLRAF
                     APAYQERFERVDPAAIRVRLQQAREELRGEGSFAPAP"
BASE COUNT          584 a         1076 c         1315 g          608 t
ORIGIN      
        1 tccgcaacct cttcggcgca ggcgcgtggc tggcggatgc caccggccgc ccggatgcgg
       61 gagagctgcc cgcgcgctgg cgcatgggga aagacgtggg cgcacgcgtc gcccaatggg
      121 taggtgatgc gcctcttccg gggctgccgc ctgccggacc atctcctgcc gcggcggagg
      181 cagaggcgtg cgacgtgctg gtggttggcg gaggcacggc gggcgctttc gcggcaatcg
      241 ccgccgggcg cgagggcgcg aaggtcaccc tgctggaagc gagccacttc cttggcggga
      301 tcggcgccgg cggcgggatc cacatctact atcacggctt cgccggtggc atccaggatg
      361 aggtcgattc gcgcacaaac gcgctctcgg atctcttcgg cgcgaatcag cgcctcactg
      421 gcttccaccc cgatgccaag caggtcgtcc tcgaagagat ggccgcggag gcgggcgtca
      481 acgtcattta ccggagcgcg ctgatcgagg cgcgtacttc aggagggcgc gtagaggtgg
      541 ccgaggtggc gagccccacc ggagcgatcc tgtacccggc ggctgctttc gtggatggaa
      601 ccggcgacgc cgacctggcg gcgatggtcg gcgccgagtt ccagctcggg cgcgagcgcg
      661 acgggctgtc gcaccacttc tcgcaggcct accagatact ggatgccaac ggcagtattc
      721 gcctcgccaa cttcgatgcc ggttacgtcg tggcgacgga cgtgaccgac ctgacgcgtg
      781 cccggcgcct gggtctcaag cacctctggc gcgacacgta taccgccgag aaccgcctgc
      841 tgaccatcgc gccgctgctc gggctgcgac agagccggca gatcgtcggc gattacgtgc
      901 tcacgctgga tgaccagatc tccggccggc gcttcccgga tgtgatctcc tacgccaaag
      961 cccactacga taaccacgcc ttcgactacg agaatgagag cgacgaggcg gcgctctggg
     1021 tgtggctgct cggtgcctgg catgatccct tcgcgagcga ggtgccctat cgctgcctgc
     1081 tcccgaaggg gatagaggga ctcttggtgg cgtgccgagc cctctcgctg acgcacgacg
     1141 cgcacatgat gttccgcatg cagcgcgacc tgcagcgcgt gggcgaggtg gcgggtgtgg
     1201 ccgccgcgat ggcggtgaag gagggaacga cgccgcgcca ggtggacgtg cggcgcctcc
     1261 aggagaagct gttgcgctct ggcattctgc gcgaggagcc ggtggtatca gaggcgtctg
     1321 gcacggtgga gggatggatc gccgcgctgg acgcacctca gccgaaagag gcggtgtggc
     1381 agttggcgca cgccggcgag gaggtgatcg agcccctccg ggaggtgctg aagaacggga
     1441 gcgagcgggc gcgcttctgg gccgcagtgg ccctggcgat gcacggccgg cccgaggcag
     1501 ccccggagct cctccgctgc ctgatcgagc gcgcggatgt gaagccagag ggctatcgca
     1561 cggttccctt ctggcagagc gtggtggtgt tgctcggccg catcggcgat ccggcggcag
     1621 tgccggagat cgcgcgcctg cttgaggagc ccgagacgac cctcgatggc ttgatcaccg
     1681 ccatccgggc gctgggccgg attggcgacc cccaggcggt gcctgcgatc caggcgatgc
     1741 tggcacgctc ggatcttccg acggagcgcc cgttccaggt gagcaccggc ggccggtcag
     1801 gggtcagcga tgacgcgcga tgggcgctgg agctggctgc cgccgagaca ctggcgatgc
     1861 tgggggcgcc gaacgcgtcg ctcgcggaga agcacctaag tgacccgcgt gcctatgtcc
     1921 ggcgctatgc agagcgcgtc cggcggatgt ctctggtgcc gatcgggttc taatcgatcg
     1981 ccggtctcgc cgccggcatg tgccgtgggg cggtatcgcg cgggtgcaaa cccggcgcca
     2041 cgcgtgtggg cggccatccc catccgcgcc cgtcccttca gccgccggcg cgggaccggc
     2101 gggcaggaag gaatgccggg tgggcttcat cttggaatca caaagcgagt gcgaagcggc
     2161 tattgactcg gcggcgggac cgtgctatac tactggtgcg tcggggcgta gctcagcttg
     2221 gtagagcact tggatggggg ccaaggggtc gctggttcaa atccagtcgc cccgaccagt
     2281 ctaacaaaca acaggtaacg cgcgggtttt ggcagccagc tgcctcccac cgggagggtg
     2341 agagcatcta gacagctgga acggccggaa ccgcggaggc agttgaaccc ttgggcttgc
     2401 cgttcgatga tgggcaagcc caagggtttt ttgttgccac gccacccact cccctcggcc
     2461 cgtgactccc catcattccg aacctcctcc ggtattcccg gcacaccagt accttctcgc
     2521 catcaacccc ttgcgaagcc gggcatctcg cgcgcagtga aacgatgcgt ccagtcagtt
     2581 ggtggcggga tgcgcattgg ttgaagtctc tttgttctgg cctggcaaaa agaggtccgg
     2641 tggctcgtct atagggtagg agagagcgcg cggcagcgga acacaccccc ggggaggtgc
     2701 gaaagagtgc gaggtcattc agagcaggtg gtcgagccgg atctgctaga gcgggtctgg
     2761 atggataggc tgcgagtcca cgatagcgcg gcgtgggatg agctggtgga gcggttcaac
     2821 gcgccgctga cgcacctggt gtgtagcctg ttgggcgggt ggtgcgaaga tgtgcccgac
     2881 atcgtccagg attctttcgt gaaggcgttc ggctcgatcg ctcgcttccg aggggagtgc
     2941 tctctccgca cctggctgtt ccgcatcgcg gtgaacctct gtcgtgacta ccagaagagt
     3001 gcgtggaggc ggcgcgtgga tctgcaaggg aatccatccg aaacgcgctt agtagaaccg
     3061 cagggagtcg cggagcagcc gttccgggac gtggagtggg agcagttgcg gctggcgctg
     3121 tcccgagttc ctgagaagga acgggtgtgc gtggaactgc ggtttatcta ccagatgacc
     3181 gggcgagaga tcagcgctgt cgtgggttgc accgagagca cggtttggtc gcgcatctat
     3241 gctggtctga agcggttgcg cgcggccttg agtgaagagg agatgaccgg caaatgaaca
     3301 cgggatggat gcggctcaga tgccggtact ggagagctca cttgataaag gcggttgcgg
     3361 gatgcctgcc ggagcgacgg cgcagggggc tcgagcggcg catcgcgcgt tgcgatgcgt
     3421 gtcgccagga tcgagagcag ctggaggaga cagctggcct gttgcgcgcg ttcgcgccgg
     3481 cctaccagga gcgatttgag cgggtggatc cggcggctat cagagtgcgc ctgcagcagg
     3541 cgagagagga gctccgtgga gaaggaagct tcgccccggc tcc
//
LOCUS       DAOSCV010000428         3576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16600_length_3576_cov_3.284294, whole genome shotgun sequence.
ACCESSION   DAOSCV010000428 DAOSCV010000000
VERSION     DAOSCV010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16600_length_3576_cov_3.284294"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PLY56_10975"
     CDS             <1..517
                     /locus_tag="PLY56_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22048.1"
                     /translation="DYARLARDIAHATREAMLEYGCTHSSADRSNLWVSQNLWRDFVA
                     AYMGLDLLDMASRYWAFEVMENTVGRGGCFIDTYGWNTLNFYPRGAASVGAFAAAAGL
                     SVDRVAGQVALSPVRVPLRVPLLPLADWEQGAVPWLDVSLVDGEVRARILRPELLKGF
                     TVTIFGKPVKL"
     gene            549..788
                     /locus_tag="PLY56_10980"
     CDS             549..788
                     /locus_tag="PLY56_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22049.1"
                     /translation="MKLGLVTYNLASDWDVPTIIERCQRTGFEGVELRTSHAHGVEPD
                     LSPMARAEVRQRDAVGSGERLRVSLAGPCRGPRAD"
     gene            715..1302
                     /locus_tag="PLY56_10985"
     CDS             715..1302
                     /locus_tag="PLY56_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ22050.1"
                     /translation="MTLWGLGSVCEFHSPDPAVVREQIEMCRSFVKLAADVGACGVKV
                     RPNGLAEEQGIPVERTLEQIGLALRECGQAAADAGVEIWLEVHGRGTSHPPYIRTILD
                     HCDHPAVGACWNSNDTDVKDGSIAEYFDLLAPDIRSVHITELWREQYPWRELFADLNR
                     IGYDRFTLAEVPANADPERLMRYYRALWLELSRPA"
     gene            complement(1315..2505)
                     /locus_tag="PLY56_10990"
     CDS             complement(1315..2505)
                     /locus_tag="PLY56_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22051.1"
                     /translation="MDEALERTIAAIHRSPCQCVIAAAGTGSQALAWLIRVAGASHTL
                     LEARLPYAKASLRDLLGHRAAHCVSAETATAMARWSLSRALELREGDVPVLGVGCTGA
                     LSTTRPRRGMHGVELAILSPGEGPEQEHQAHYTLRLEKGARTRGEEEDACSLLLIHAL
                     ALACGVIPGFTLPLLPGDEMHHEGDLSPLTDLLQGRARHILVSQDGRALADAAVTGGV
                     LPGSFNPLHEGHRRLAEVAAELLQAPVTFELSVENVDKRALTEGEALRRAQQFWGWAP
                     VALTRAPTFREKARLFPGCTFIVGYDTAVRIVAERYYASAADMQEALREIRDAGCRFL
                     VAGRAHAGAFHTLADVPVPEEFRSLFQEVPPELFRLDISSTELRARQQSGAGAILERP
                     LPGA"
     gene            2661..>3576
                     /locus_tag="PLY56_10995"
     CDS             2661..>3576
                     /locus_tag="PLY56_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ22052.1"
                     /translation="MDYSSLLEMFFRVARRHPAEIALAAKVDGRYQGQSYAAVEAQVR
                     RLGAALVRLGVQAGDRVALMSENRPEWALTDLATYAAGGVLVPLYSTLPPPQVEYILR
                     DSGASIFVVSGKERIESALALRERLPNLRHIIAIGQYAPKGTTAFDSLLAQPADPDSE
                     AESERRSGALNRDTLATIVYTSGTTGEPKGAMLTHGNILANLEGCDPIFRLDEKDVFL
                     SFLPLSHIFERTAGYYLAIRNASAIYYAESVFTVAKNLEEVRPTIMMSVPRLYENIQQ
                     RVLDTASKATALQRRLFEWALQVGYEHSR"
BASE COUNT          579 a         1145 c         1253 g          599 t
ORIGIN      
        1 ggactacgcg cgcctcgccc gcgacatcgc ccacgccacg cgcgaggcga tgctggagta
       61 tggatgcacc cactcgagcg ccgacaggag caacctgtgg gtgtcgcaga acctgtggcg
      121 tgattttgtc gccgcctaca tgggcctcga cctcctcgat atggcgtcgc gctactgggc
      181 cttcgaggtg atggagaaca cggtggggcg gggcggttgc ttcatcgaca cttacggctg
      241 gaacacgctc aacttctacc cgcgcggtgc ggccagcgtg ggcgccttcg cggcggcggc
      301 tggtctcagc gtggatcgcg tggcgggcca ggtggcgctc tccccggtgc gcgtgccttt
      361 gcgcgtgccg ctgctcccgc tcgccgattg ggagcaaggc gctgtgccct ggctggatgt
      421 gtctctggtg gatggcgaag ttcgggcgcg catcctgcgg cccgagttgc tgaaggggtt
      481 caccgtgacg atctttggca agccggtgaa gctctagcgg atcgctggag agaaggagca
      541 tcgggatcat gaagctgggt ttggtcacct acaatctggc cagtgattgg gacgtgccga
      601 cgattatcga gcgctgccag cgcaccgggt ttgagggcgt ggagctgcgc acttcgcacg
      661 cgcatggggt ggagcccgat ctctcgccta tggcgcgcgc ggaggtccgc cagcgtgacg
      721 ctgtggggtc tggggagcgt ctgcgagttt cactcgccgg accctgccgt ggtccgcgag
      781 cagattgaga tgtgtcggtc gttcgtgaag ctggccgccg atgtgggcgc gtgcggcgtg
      841 aaggtgcgcc cgaacggcct ggcggaggag caggggatcc ccgtggagcg tacgctggag
      901 cagatcggac tggcgctgcg cgagtgcggc caggccgcgg cggatgccgg cgtggagatc
      961 tggctggaag tgcatggccg gggcacttct cacccgccct acatccgcac catcctcgac
     1021 cactgtgatc acccggcggt cggcgcctgc tggaactcga acgacaccga cgtgaaggac
     1081 gggagcatcg cggagtactt cgacctcttg gcgcccgata tccgctcggt gcacatcacc
     1141 gagttgtggc gcgagcagta cccctggcgc gagctcttcg ccgacttgaa ccgaatcggc
     1201 tatgatcgct ttacgctcgc ggaggttcct gccaacgccg atccggagcg tttgatgcgc
     1261 tactaccggg cgctctggct ggagctgagc cggcccgcgt agcaggcgtg tcgcttacgc
     1321 gcccggcagc gggcgttcca ggattgcacc cgcgccgctc tgctgccggg cgcgcagctc
     1381 cgtcgaggag atatcgagcc ggaagagctc cgggggcacc tcctggaaga ggcttcgaaa
     1441 ctcctccgga accggcacat cggcgagggt atgaaaagcg cctgcgtgcg cgcggccggc
     1501 cacgagaaag cggcagccgg catcgcggat ctcgcgcaga gcctcttgca tatcggctgc
     1561 gctggcatag tagcgctcgg ccacgatgcg cacggctgtg tcgtagccga cgatgaaagt
     1621 gcacccaggg aagagacggg ccttctcgcg aaacgtggga gcgcgcgtga gcgcgacggg
     1681 cgcccatccc cagaactgct gcgcccggcg caacgcctcc ccctcggtga gcgcgcgctt
     1741 gtccacgttc tccaccgaga gctcgaaggt gaccggggcc tgcaatagtt cagcagctac
     1801 ctcggccagg cggcgatggc cttcatgcag cgggttgaag gagccgggaa gcacgccgcc
     1861 cgtcactgcg gcatctgcca gggcgcggcc atcctggctg accagaatat ggcgcgcgcg
     1921 cccctgaagc aggtcggtga gaggggagag gtcgccttca tggtgcatct cgtcgccggg
     1981 cagcagcggg agcgtgaagc ctgggatcac cccgcaagcg agtgccagcg cgtggatcag
     2041 gagcaggctg caggcatcct cctcctcccc gcgcgtgcgt gcccccttct ctagccggag
     2101 cgtgtagtgg gcctgatgct cctgctcagg gccctcgccc ggcgagagga tggcaagctc
     2161 gaccccgtgc atcccccggc gagggcgcgt ggtggagagc gccccggtgc agccgactcc
     2221 gagtaccgga acatccccct cgcgcaattc gagcgcccgc gagagtgacc agcgtgccat
     2281 cgccgtggcg gtctcggcag agacgcagtg cgccgcgcgg tgccccagaa gatcgcgtag
     2341 cgacgctttg gcatagggga gccgggcctc gagcagcgtg tgcgaggcgc ccgccacgcg
     2401 aattagccag gcgagcgcct ggcttcctgt gccggcggcg gcgatcacgc attggcacgg
     2461 cgagcggtgg atcgcagcga tggtgcgttc aagagcctcg tccataactc ttccgttcgc
     2521 tctgcacccg ccttttcctg gaacaaggag aacgagcaag cgtccgagaa gtatgggcgt
     2581 ggcaatgtgc gctcttactg ggaccccagc aggggcgtgg gagcattgcg cggccatgac
     2641 cgcggtctgg aggcattggg gtggattata gcagcctgtt agagatgttc ttcagggtgg
     2701 ccaggcgaca cccggccgag attgccctgg cggcgaaagt ggatgggcgt taccagggac
     2761 aaagctatgc ggctgtcgag gcgcaagtgc ggcgactggg tgcggccctg gtgcgtctgg
     2821 gtgtacaggc gggtgatcgc gtggccctga tgagcgagaa ccgtcccgag tgggctctca
     2881 cggacctggc gacctatgcc gccgggggcg tgttggtgcc gctctactcc acgctgcctc
     2941 cgccgcaggt ggagtacatc ctccgcgact cgggcgcgag catcttcgtc gtctccggca
     3001 aggagcgaat cgaaagcgca ctggccctgc gcgagcgcct ccccaacctg cgccacatca
     3061 tcgccatcgg ccagtacgcg ccgaagggga cgaccgcgtt cgacagcctc ctggcgcagc
     3121 ccgcggatcc cgactcggag gcggagagcg agcgacgctc gggcgcgctc aaccgcgaca
     3181 cgctggcgac gattgtctac acctccggaa cgaccggcga gccgaaaggc gccatgctca
     3241 cgcatggcaa catcctggcg aacctggagg ggtgcgaccc gattttccgg ctcgatgaga
     3301 aggatgtctt tctttcgttc ctcccgctgt cgcacatctt cgagcgcacc gccggctact
     3361 acctcgcaat ccgcaacgcc tccgcgatct actacgccga gagcgtcttc accgtggcga
     3421 agaacctgga agaggtgcgc cccacgatca tgatgagcgt gcctcgcctc tacgagaaca
     3481 tccagcagcg tgtcctcgat acggcgagca aggccacggc cctccagcgg cggcttttcg
     3541 agtgggcgct ccaggtgggc tacgagcata gtcgct
//
LOCUS       DAOSCV010000429         3567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16668_length_3567_cov_2.601082, whole genome shotgun sequence.
ACCESSION   DAOSCV010000429 DAOSCV010000000
VERSION     DAOSCV010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16668_length_3567_cov_2.601082"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..574
                     /locus_tag="PLY56_11000"
     CDS             2..574
                     /locus_tag="PLY56_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22053.1"
                     /translation="MAAILFPVFARARENARKSTCQSNLKQIGMATMQYLQDYDEMFP
                     VYWHGSGHNLNVQTRLEPYMKNTQLWVCPSNSAPYYYYWDNPSGTGAVTGPKGSYGWN
                     ITLGYNANVRSMAQVQQPSETGMWADSQGNGSSYQFTILQENSLYRTSVPHSDGGNMC
                     YVDGHVKWQNKNYLRAGMPTATRAEWDIAL"
     gene            complement(666..1844)
                     /locus_tag="PLY56_11005"
     CDS             complement(666..1844)
                     /locus_tag="PLY56_11005"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ22054.1"
                     /translation="MEHRFHEEEQPAGPPLVQPVDPEAVDARRRETYLSYEGFLRGLL
                     EELDRRRPEEWQRDYSSLVAYERSVRPMRDRLKRMLGFWVEPEARPAPRTWDHEVLLE
                     TDDFTATRFSLEILPGLQSYAVELIPTSPGPRPGLIAQHGYGGTPEAVCGFTSHANKP
                     DYSYRSLGIRAARHGFHVIAVAHPTGYGRPEESITWPLPGHEDLPPQYGKNRLHRMAI
                     MAGGTLFGLDMMASSRGVDLLTTRPDVDPERIGMYGLSQGGQSALYLPALDERIQASV
                     SSAYFNHRFPKLIGPHRARTFLDSAEEDKFFSKVIRCFSDCDLVSLIAPRAFAVEAGL
                     HDSSVDFEKSADEFVWAREHYERLGIADRIEFIAHTGGHVSSTRRAFEFLLEHLGSAG
                     "
     gene            complement(2000..>3567)
                     /locus_tag="PLY56_11010"
     CDS             complement(2000..>3567)
                     /locus_tag="PLY56_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22055.1"
                     /translation="GGRAEAALKIEFYTEKGENTSGRYSQRLTPGSEWRELAVEAKAP
                     PDSIRAAILVRQFGSGEVWFDDVAFFRVGEAPVITVDPPRIAAVAGTPGRFEVDLRLR
                     DAGQGTPPRPEVMLLTSEGRSLPGVTMDLAADGPRLHRGTIHAPALAPGDYVLRFTLP
                     NGHIDCRLAITPAKRKPRNLTEEGVILANGKPFFPIGLYHVSPSEYALLAEHGFNCVQ
                     GLNTTNEARFAESLELARKAGILVDVPFYIRGEVAKNLPLSLDRIRRFKEHPAVLNWK
                     IIDEPDLRPEIIDEVADAYRKLREADWDHPLLLTIASPPSYGHWAHFCDILQIDPYPI
                     PKQPLTMVSDYCDRAKAVLQPWQNLTAVLQCGWLPGPPANQPTYEQARAMVYLAVIHG
                     AKGIFWYSMHDPGWDLTKTPLWPRFKELNAETAELGALAILGKPVEVACDTEEVHAAA
                     WEREGRVYILATNPGKDARTATLKPRAGRWAAGRDGAALRVLRGEAEFKMREGTLLLT
                     LPAVGSTLLEIGG"
BASE COUNT          629 a         1176 c         1164 g          598 t
ORIGIN      
        1 cctggccgcg atcctctttc cggtgttcgc gcgggcgcgg gagaacgcgc gcaagtccac
       61 ctgccagtcg aacctgaagc agatcggcat ggccacgatg cagtacctgc aggactatga
      121 cgagatgttc ccggtctact ggcatggcag tggtcacaac ctgaacgttc agacgcggct
      181 ggaaccctac atgaagaaca cgcagctgtg ggtgtgcccg tctaattcgg cgccctacta
      241 ctactattgg gacaacccca gcggcacggg agccgtaacc gggcccaagg gatcttacgg
      301 ctggaatatc accttgggtt acaacgccaa cgtccgctcg atggcgcagg tgcagcagcc
      361 ctcggagaca gggatgtggg ccgactccca ggggaacggc tcctcctacc agttcaccat
      421 cctgcaggag aacagcctct accgcacttc cgtgccccac tcggatggcg gcaacatgtg
      481 ctacgtggac gggcacgtga agtggcaaaa caagaactac ctgcgcgcag ggatgcccac
      541 cgccacgaga gcggaatggg atatcgccct gtaagcggtc tccgtaatag cctcgaagag
      601 tggcaggcgg ggcgcggttc cggcacgcgg ggccgcgccc cgctctctct ggttctaccg
      661 gtctctcacc ccgcacttcc caggtgttcc agcaagaact cgaaggcccg gcgggtggaa
      721 gagacgtgcc cgcccgtatg cgcgatgaac tcgatccgat ccgcgattcc caggcgctcg
      781 taatgctccc gagcccacac aaactcatcc gcggacttct cgaaatccac cgaactgtcg
      841 tgcagccccg cctcgacggc gaaggcacgc ggggcgatca gcgagacgag gtcgcagtcc
      901 gagaagcagc ggatcacctt ggagaagaac ttatcttcct cggccgaatc gagaaaggtg
      961 cgcgcccggt gcgggccaat cagctttgga aagcggtggt tgaaataggc gctgctcacc
     1021 gacgcctgga tccgctcatc caaggccggt agatagagcg cgctctgccc gccctgagat
     1081 agcccgtaca tgccgatccg ttcaggatcc acatccgggc gcgtggtcag aagatccacg
     1141 ccgcgggaag aggccatcat gtcgagcccg aagagggtgc ccccggccat gatcgccatg
     1201 cggtggaggc ggttcttgcc atactgcggc gggaggtcct cgtgcccagg gaggggccaa
     1261 gtgatgctct cctcgggccg gccgtaaccg gtggggtggg ctaccgcgat cacatgaaag
     1321 ccgtgtcgtg ccgcgcggat ccccagtgag cggtaggagt agtccggctt gttggcatgg
     1381 gaggtgaagc cacacaccgc ctccggcgtg cccccatagc cgtgctgggc gatcagccct
     1441 gggcgcgggc cgggcgacgt ggggatgagc tccacggcgt agctctgcag gccgggcagg
     1501 atctccaggg agaaccgggt ggccgtgaag tcgtcagtct ccaggagcac ttcatggtcc
     1561 catgttcttg gcgcagggcg cgcctccggc tccacccaga agccaagcat gcgtttcagg
     1621 cggtcgcgca tcgggcgcac cgagcgctcg taggccacga ggctggagta gtcacgctgc
     1681 cactcctcgg ggcggcggcg gtcgagttct tccaggaggc cgcgcaagaa gccctcatac
     1741 gagaggtagg tctcgcgccg gcgcgcatcg accgcctcgg gatcgaccgg ttgcaccaac
     1801 ggtggtcccg ctggctgctc ttcctcatgg aaacggtgct ccatcgttca ctccctgaga
     1861 ttgctggcgc gcacgcgcca ccgaacgcga gcgtgccggg gcaccacgat accgcggccg
     1921 gccctcgccc cctcggcatc gtggttcctt tgtctttaat gagggaagga cacctctccc
     1981 caccaaccgg gatggcgcat cagccgccta tctctagcag ggtgctgccc acagccggca
     2041 gcgtcaggag aagcgtgccc tcacgcatct tgaactccgc ctcgccgcgc agaacgcgga
     2101 gcgccgcccc atcccggccg gccgcccacc ttcccgcccg cggcttgagg gtggccgtgc
     2161 gcgcatcctt gcccgggttg gtcgccagga tgtagacgcg gccctcgcgc tcccaggcgg
     2221 cggcatggac ctcctcggta tcgcaggcca cttcgaccgg cttgcccaga atggcgaggg
     2281 cgcccagctc cgccgtctcc gcgttcagct ccttgaagcg cggccagagc ggcgtcttgg
     2341 tgaggtccca gccggggtcg tgcatcgagt accagaagat ccccttcgcc ccgtggatca
     2401 ccgccaggta gaccatcgct cgcgcctgct cgtaggtcgg ttggttggcc ggcgggccgg
     2461 gaagccaccc gcattgtaac acggcggtga ggttctgcca cggctggagg acggctttcg
     2521 cccggtcgca atagtccgat accatcgtca gcggttgctt tgggatcggg taggggtcaa
     2581 tctgtagaat atcgcagaag tgcgcccaat gcccgtaact ggggggactc gcaatcgtga
     2641 ggagaagcgg gtggtcccaa tccgcctcgc gcagcttgcg ataggcatcc gccacctcgt
     2701 caatgatctc ggggcgcagg tccggctcat cgatgatctt ccagttgagt acggccggat
     2761 gctctttgaa gcgccggatc cgatccagcg agagagggag gttttttgcc acctcccccc
     2821 ggatgtagaa cggcacatcg accaggatgc ccgccttccg ggccagctcc agcgattcgg
     2881 caaacctggc ttcgttggtg gtgttgaggc cctgcacgca gttgaagcca tgctcagcca
     2941 gaagcgcata ctccgagggc gacacgtggt agagaccgat ggggaagaac ggcttgccat
     3001 tggcgaggat gacgccctct tccgtcaggt tgcgcggctt gcgcttcgcc ggcgtgatgg
     3061 caaggcggca atcgatatgg ccattgggaa gggtgaaacg cagcacatag tcacccggcg
     3121 ccagcgccgg cgcatggatc gtgccccggt gaagccgcgg gccgtcggcg gccagatcca
     3181 tcgtcacacc gggcagcgac cgaccctcgg aggtgaggag catcacctcg gggcggggtg
     3241 gcgtgccctg cccggcatcc cgcaggcgaa ggtccacctc gaatcgtccg ggcgtgcccg
     3301 cgacggcggc aatccgcggc gggtcgaccg tgatcacggg cgcttcgccg acgcgaaaga
     3361 aggcgacatc gtcgaaccac acctcgccgg agccaaactg acgcacgaga atggccgccc
     3421 ggatactatc cgggggcgct ttcgcctcga cggcgagctc tcgccattca ctccccggcg
     3481 tcagccgctg ggagtagcgg ccactggtgt tctctccctt ttcggtgtag aactcgatct
     3541 tgagcgcggc ctcggctcgc ccgccct
//
LOCUS       DAOSCV010000430         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16679_length_3565_cov_3.925356, whole genome shotgun sequence.
ACCESSION   DAOSCV010000430 DAOSCV010000000
VERSION     DAOSCV010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16679_length_3565_cov_3.925356"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..1844)
                     /locus_tag="PLY56_11015"
     CDS             complement(345..1844)
                     /locus_tag="PLY56_11015"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOJ22056.1"
                     /translation="MRSEFIHRLVSLAAELADSVPGGKTSSEELGPNARWYASSWQRA
                     GGIDVFLARDGVERCLVVAGAGPEAARVPGEVVATGERPVKVAAYSSEACAALRALFP
                     WTAPRALPKHRPSFGCGDRLGVATPGQLRGLRHTRAALVLAQQSVRELTLTGRSYAEV
                     VDAATWGVFQEGYQEGFGADGDHLKTADEVRALLDAGATFITLDLSLCLGCASSLPEM
                     PDAVRRLEGNSWELAGERLEANPDDLRGFWRVYGPTFAFIEEADALCRQARGVGGYDL
                     EVSVDETAHATRPLDHLLLATELARRGIAVTSIAPRFPGEFQKGIDYIGDRERLEREV
                     AIHAEIAHRFGYKLGVHSGSDKFAVFPLFGRHGGDRLHVKTAGTSWLEALRVVAATDP
                     ALFRAIWTQATEGFPRAREYYHIGTSLADVTGKADVPDSELPHLLNDDACRQFLHITY
                     GEILKAPAPGGGTLSDALQENLAQNEARYAHGLEAHFRRHADGLGLTSS"
     gene            complement(1936..2133)
                     /locus_tag="PLY56_11020"
     CDS             complement(1936..2133)
                     /locus_tag="PLY56_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22057.1"
                     /translation="MEVVLTERERDLLIEMLDSEISEVRSELYHAEDAETKEMFRDRE
                     ELAKRLRAKLEGSALREPVVT"
     gene            2573..>3565
                     /locus_tag="PLY56_11025"
     CDS             2573..>3565
                     /locus_tag="PLY56_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ22058.1"
                     /translation="MAMTMTEKILARAAGREAVRAGENVWVNVDVLLTHDVCGPGTIG
                     VFQEHFGKDARVWDKEKVVIIPDHYIFTADAKARRNVEILREFVAAQGIPNFYDVGTE
                     RYKGVCHVGLPEGGHTRPGEVLFGTDSHTCTHGAFGEFATGIGNTEAGFILGVGKLWV
                     RVPETMRFIFDGSMPPYLMAKDLILHVIGEIGVDGATYRAMEFAGDAVYSLNMEERMT
                     LCNMAIEAGGKTGIIAPDQVTCDYVRARTSKPFTVFTNDEGATFHSEHIFNVAELEPT
                     VALPHSPDNRALARELSDVRIDRSYIGSCTGGKTTDFIAAARILNGRTVAVDTFI"
BASE COUNT          593 a         1204 c         1147 g          621 t
ORIGIN      
        1 gccagggtga ccgcccccgc cggcagcgcc agagttgcca gcgccaccag ccatacgaca
       61 cggctcagcg ctctgagcca gatcctgcct cccggttgag gcatggaacc gagtttgggc
      121 atcgcgtacc tcctgattcc ttcggcatgg cctgctctgg gggcaactgg cgccatgcca
      181 ccgctcccca gccgcgctct accgcaggcg gcatcccgcc gctcttagag cgtctgttac
      241 gccaggatgc gggaggactc cttcgcgccc ggatctgcct ggatcaccgt ggcagtcggg
      301 tgctggtccc atgcggctct cgcctgggaa gccttttgcc gcgctcagga cgaggtgaga
      361 ccgaggccat cggcgtggcg gcggaagtgc gcttccaggc cgtgggcgta gcgcgcctca
      421 ttctgggcca ggttttcctg gagggcatcg ctcagggtgc cgccccctgg cgcaggcgct
      481 ttcaggatct ccccgtaggt gatgtgcagg aattgccggc aggcatcatc gttcagaaga
      541 tgggggagtt ccgagtccgg cacgtcggcc ttgccggtta catcggcgag cgaggtgccg
      601 atgtggtaat actcgcgagc ccgagggaag ccctcggtcg cctgcgtcca gatcgcccgg
      661 aagagcgccg ggtcggtggc ggcgacgacg cgcagcgcct ccagccagct tgtgccggcg
      721 gtcttcacgt ggaggcggtc gccgccgtgg cggccaaaga gcgggaagac agcgaactta
      781 tcgcttcccg agtggacccc gagcttgtaa ccaaagcggt gcgcgatctc tgcgtggatt
      841 gccacctcgc gctccaggcg ctcccggtcg ccgatgtaat cgattccctt ctggaactcg
      901 ccggggaagc gcggcgcgat gctggtgacc gcgatcccgc gccgcgcgag ctcggttgcg
      961 agcaggaggt ggtcgagggg gcgggtggcg tgcgccgtct catcgacgct cacttccagg
     1021 tcgtacccgc cgacgccacg cgcctgccgg cagagggcat cagcctcttc gatgaaggca
     1081 aaggtgggcc cgtaaacgcg ccagaatccc cgaaggtcat cagggttggc ctctaggcgc
     1141 tctcctgcca gctcccagga gttgccctcc agccggcgca ctgcgtccgg catctcggga
     1201 aggctgctgg cgcacccgag gcagaggctc aagtcgagcg tgatgaaggt ggcgccggcg
     1261 tccaggagag cgcgcacctc atctgccgtc ttcaggtggt cgccatccgc gccgaagccc
     1321 tcctggtagc cctcctgaaa gacgccccat gttgccgcat cgaccacctc agcatagctt
     1381 cggccggtga gtgtcagctc gcgcaccgac tgttgggcca ggaccagcgc tgcccgagtg
     1441 tgccgtaggc cgcggagctg cccgggagta gccacgccaa gccgatcacc gcacccaaag
     1501 gaggggcggt gcttgggcag cgcgcggggc gcggtccacg ggaagagcgc gcgcagcgcg
     1561 gcgcacgcct ccgaggagta cgccgccacc ttcaccggac gttcacccgt tgccaccact
     1621 tcgcccggca cgcgggcagc ctccggaccc gcccccgcga ccaccaggca gcgttccacg
     1681 ccgtcgcgtg ccaggaacac gtcaatgccc cctgcgcgct gccacgaaga ggcgtaccaa
     1741 cgggcgtttg gcccgagctc ttccgaggac gtcttgccgc ccggcacgct atcggccagc
     1801 tctgccgcca aactcaccaa tcggtggatg aactcgctac gcaccagctt ccctcctccg
     1861 ctgcgtttct gaacctcagt tgactcttgt ctcaacgttc cccaggcagc ggcgaagaac
     1921 ctgccggtgc gcgtctcagg taaccacggg ctctctcagc gcgctccctt ccagctttgc
     1981 cctcaggcgt ttcgccaact cctcgcgatc gcggaacatc tccttcgtct cggcatcctc
     2041 ggcgtggtaa agctcagaac gcacctcgct gatctccgaa tccagcatct cgatcagcag
     2101 atcgcgctct cgctcggtga gaacgacctc caaggctctc gtctcctttc gcatcggttc
     2161 gccagtccgc gccaccaacc cggagctacc gctccaacaa gctggccaaa cccgatgccg
     2221 ttccggggtg gcgtgctctg tggggcacgc agaggcttta ggcccgtgcc cgctgggaga
     2281 caccgccggc catcaggccc gggcagtgcc ggcggtttga tacccgcgga cgcgccttgc
     2341 caatccgcgg gcgcatcggc tataatgaag tagtgcctcg gaatctttgg gccgagcgcg
     2401 tattgtttga gcgatctcaa gagccagcag cctgccgcca acgcgtgggg gacagtgttt
     2461 cacgacgggg tgtgccgccc gcgccaccca accagagcgc cggcgtgcgc cagaaagagc
     2521 ggatcctgat cctcattgca ggcgtggcat caccattcag ggagccccta tcatggcgat
     2581 gacgatgacg gaaaagatcc tcgcccgtgc agccggccgc gaggccgtgc gcgccggcga
     2641 gaatgtttgg gtcaatgtgg atgtgctgtt gacccacgac gtctgtggcc ccggcaccat
     2701 cggcgtcttc caagagcact tcgggaagga tgcgagggtc tgggataagg agaaggtggt
     2761 gatcatcccc gatcactaca tcttcaccgc cgatgcgaag gcccggcgca atgtggagat
     2821 cctgcgcgag tttgtggcgg ctcagggaat ccccaacttc tacgacgtgg gcacggagcg
     2881 gtataagggc gtctgccacg ttggtcttcc agagggcggg cacacccgtc ccggcgaggt
     2941 gcttttcggc accgactcgc acacctgcac gcatggcgct tttggtgagt ttgctaccgg
     3001 cattggcaac acggaggcag gcttcatcct gggcgttggc aagctatggg tccgcgttcc
     3061 cgagacgatg cgcttcatct tcgatggaag catgcccccc tatctgatgg ccaaggacct
     3121 gatcctccac gtcatcggtg agatcggcgt ggatggcgcc acctatcgcg ccatggagtt
     3181 cgcgggtgac gccgtctatt cgctcaatat ggaagagcgg atgacgctgt gcaacatggc
     3241 gatcgaggcc ggcggtaaga ccggcatcat cgctcccgat caggtgacct gcgactacgt
     3301 gcgcgcgcgc acgagcaagc cgttcaccgt tttcacgaac gacgaggggg ccacgttcca
     3361 cagcgagcac atcttcaacg tggcggaact ggagccgacg gtggcgctgc cgcactctcc
     3421 cgataaccgg gcgctcgccc gagagctctc ggatgtgcgg atcgaccgct cctatatcgg
     3481 ctcttgcacc ggcgggaaga ccaccgactt tatcgcggcc gcgcgcatcc tcaacgggcg
     3541 caccgtggcg gtggatacgt tcatc
//
LOCUS       DAOSCV010000431         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16680_length_3565_cov_3.708262, whole genome shotgun sequence.
ACCESSION   DAOSCV010000431 DAOSCV010000000
VERSION     DAOSCV010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16680_length_3565_cov_3.708262"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2201
                     /locus_tag="PLY56_11030"
     CDS             <1..2201
                     /locus_tag="PLY56_11030"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HOJ22059.1"
                     /translation="ALGEAAESARLELLSGDGQRVAEQTLPLKETTTLLELDMKPLPR
                     GRYQARLTLFSGGKPQAVETLALRKETEETWESAARIAKKDTLLPPFTPLKVTPAQGA
                     QPARIAPYGREYLLEGSGLPSQIHSDGHPLLTGAVRVVAEGAGALAPKRVALERATEV
                     QARLTGGLDGDGIGVETAVVARYDGTLWTTLRFQPKKPVRLERLRVEIPIREAEARYL
                     AYIAMGRVDNKRLGYDALPAGEGVVWRREFLPSIWIGTEERGLGWYAESDEHWDTDGE
                     EALTVERRGGTALLCMNVIRQPREVTEPFTIEFGLQATPVRPLAPDWRANQWVSSAEI
                     TQFFLHLRKNPYPRPEVEGKSPSGKVCYLYAYHDYFTSTLPKDPEELREMISRAKAHG
                     LFCTPYTDTTYLPESFGDLWMREAELRSLPGARAVSYGPVSNASVCHNGPFGDWFVWY
                     VAHLAREYGSNGIYLDDTWTYGCMNAAHGCGYVGRDGKRRPTYPLRARHETYRRLREV
                     FAATGQPFWITMHMSGGRVPPLPTYGDCLLLAEERYHDVAKNPDYCENTTPEEWRASF
                     ATEAWGIPVVVLPQFKMNANWMKDPELAHRLMAAVVPHDAMIWPIFSETETVTSYRRA
                     LMEFGIGEADTRFIPYWKKDAGLECAHPGVKLSAYQRPGKLLLLLANWTAEDASGVTV
                     SLAPALLEKGAPTRARDALSGEAVPVTAGGRLVLAVPAKRVRLVEVVTGR"
     gene            complement(2343..3221)
                     /locus_tag="PLY56_11035"
     CDS             complement(2343..3221)
                     /locus_tag="PLY56_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22060.1"
                     /translation="MKKTLEKVAWTFAGSAITLGIVLTYGVATAQQPPAGPPGPGRPG
                     GGGRNFVMGTVVQTDLQHNAVQVNTPQGQRWVQLPQGMTITKRETVSLNDLAVGQTIT
                     VSGIPAEILADSLEIVPGGAATPGAAAAPGAPAAPGAPAPGAPAGQGFPGRDWMQGRT
                     AITATVAAVNPLVLTGPNNAKITVITNEQTKIAQRKQVSVQELVEGEHFAAFGQPDNR
                     DVLQARVAVTGASQADVQAMMRDMGRDGMGFRGPRGEGGWRPEGAREGGRRPEGGARP
                     GGARPGGARRGGGQAQ"
BASE COUNT          544 a         1192 c         1211 g          618 t
ORIGIN      
        1 atgccctggg cgaagccgcg gagtcggccc ggctcgagct cctttcgggg gatggccagc
       61 gcgtggccga acagaccctg ccgctcaagg agacgacgac gctcctcgag ctcgacatga
      121 agccgcttcc tcgggggcgc taccaggccc ggctgacgct cttctctggc gggaagccgc
      181 aggctgtcga gacgcttgcc ctgcgcaagg agacggagga gacctgggag agcgccgcgc
      241 gcatcgcaaa gaaggacacg ttgctgccgc ccttcacgcc gctgaaggtg acgccggcgc
      301 agggcgcgca gccggcccgc attgctccgt atggccgcga gtacctcctg gaaggctcgg
      361 gtctgccctc ccagatccac tccgacgggc accccttgct caccggcgcg gtgcgcgtgg
      421 tcgccgaggg tgcgggcgcg ctcgccccga agcgcgtcgc cttggagagg gcgaccgagg
      481 tgcaggcgcg cctcaccggt ggtctggatg gagacgggat cggggtagaa accgccgtgg
      541 tggcccgtta cgatggtacc ctgtggacga cgcttcgctt ccagcccaag aagccggtcc
      601 gcctggagcg gctgcgcgtg gagattccga ttcgggaagc cgaggcgagg tacctggcct
      661 acatcgctat ggggcgcgtg gacaataagc gtcttggcta tgacgcgctg ccggccggcg
      721 agggcgtggt gtggcgccgc gagtttcttc cctccatctg gatcggtacg gaagagcgcg
      781 gcctgggctg gtatgccgaa agcgacgagc actgggatac cgatggcgaa gaggcgctaa
      841 ccgtagagcg ccgcggtggt acggcgctcc tctgcatgaa cgtgatccgc cagccgcgcg
      901 aggtcacaga gccgtttacc atcgagtttg gactccaggc cacaccggtc cgccccctgg
      961 cgcccgattg gcgggcgaac cagtgggttt cctccgcgga gatcacccag ttcttcctcc
     1021 acctgcggaa aaacccctac ccgcgccctg aagtagaagg gaaaagcccg agcgggaagg
     1081 tgtgctatct ctatgcgtac cacgactact ttaccagcac gcttcccaag gatcccgagg
     1141 agcttcgcga gatgatctcg cgggcgaagg cgcatggcct cttctgcacg ccttacaccg
     1201 acacgactta ccttccggag agcttcggcg atctgtggat gcgcgaggcg gagctccgct
     1261 cgctgccggg cgcgcgcgcc gtctcttacg gtcctgtgtc gaacgcctct gtctgccaca
     1321 acggcccctt cggcgattgg tttgtctggt atgtggccca cctggcgcgc gagtatggga
     1381 gcaacgggat ctacctggat gacacctgga cctacggctg catgaacgcc gcccacggct
     1441 gcggctatgt gggccgcgac ggaaagcgcc ggcccaccta tcccttgcgc gcgcgccacg
     1501 aaacctaccg ccgcctgcgt gaggtgttcg cggcgaccgg ccagcccttc tggatcacca
     1561 tgcatatgag cggtgggcgc gtgccacccc tgcccaccta tggcgattgc ctcttgctgg
     1621 ccgaggagcg gtatcacgac gtggccaaga atccggatta ctgcgagaat accacgccgg
     1681 aggagtggcg cgccagcttt gccaccgagg cgtggggcat tccagtggtc gtcttacctc
     1741 agttcaagat gaacgccaac tggatgaagg acccggagct ggcgcaccgc ctgatggccg
     1801 ccgtggtgcc tcacgacgcc atgatctggc cgatcttctc ggagacggag accgtgacga
     1861 gctaccgccg ggcgctgatg gagttcggca ttggggaagc agacacgcgc ttcatcccct
     1921 actggaagaa ggatgccggt ctggagtgcg cgcacccggg ggtgaagctg agcgcctacc
     1981 agcgcccggg caagctcctt cttctacttg ccaactggac ggctgaggat gcctcggggg
     2041 tgaccgtctc cctggcgccg gccctcttgg aaaagggcgc acccacgcgg gcgagggatg
     2101 cgctctcggg agaggcggtg ccggtaacgg ctgggggccg actggtgctc gccgtgcccg
     2161 cgaagcgcgt ccggctcgtg gaagtggtta cggggcggta gggctcttct ccccctagcg
     2221 gctttcctgc tgtatccggg cggcggatgg caggaggagc ggcttgccct gggacagaga
     2281 agagccccgg gagggccggg ttcgcgctgc ccggaccctt cccgggggag atcaggaggt
     2341 tactactggg cctggccacc tccgcggcgc gcgcctccag gacgtgcacc tccaggacgc
     2401 gcgccacctt ccggacgcct gccgccctca cgggcacctt ccgggcgcca gccaccctcg
     2461 ccgcggggcc cgcggaatcc catcccgtcg cggcccatgt cgcgcatcat cgcctgcacg
     2521 tccgcctgcg aagcgcccgt gacagcgacg cgcgcttgca agacgtcacg gttatccggc
     2581 tggccgaagg ccgcgaagtg ctcaccctct accagctcct gcacgctgac ctgcttgcgc
     2641 tgggcgattt tcgtctgctc gttggtgatg accgtgatct tcgcattgtt cggcccggtc
     2701 agcaccaggg ggttgaccgc ggcgacggtg gccgtgatgg cggtccggcc ctgcatccaa
     2761 tcgcgcccgg ggaagccctg gccggcggga gcgccgggcg cgggggcacc gggggctgca
     2821 ggggcaccgg gggctgcggc ggcgcctggc gtggcagcgc cgccaggcac gatctccagg
     2881 ctgtcggcga ggatctcggc cggtatgccg ctcacggtga tcgtctggcc gaccgcgaga
     2941 tcattcaggc taaccgtctc gcgcttcgtg atcgtcatcc cttgcgggag ctgcacccag
     3001 cgctgtccct ggggcgtgtt gacctgcacc gcattgtgct gcagatccgt ctgcaccacc
     3061 gtgcccatca cgaagttgcg accgcctccc ccgggccgac ctgggccggg agggccggcg
     3121 ggcggctgtt gggcagtcgc cactccatag gtgaggacaa tgcccagagt gatcgccgag
     3181 ccggcgaatg tccacgctac tttctcgaga gtctttttca tggtggaaac ctcctgcttc
     3241 cagcatcttc tgccccttgc cagggggcgg atcccacccc cctgcccgac gcgcctgagc
     3301 ggtatctctc cgctccctgc cggcgagccg gggcgttcgg ctacaggtta gacgggcgtg
     3361 gtgccctttg cgttgaccct cttttcgcgg cgagtgttaa gaaagtgctc actccttcac
     3421 aggcggcctc gacgtgccca ttcggcgtat tgcgctggcc atatttcccg ggtggcaagc
     3481 gtcatctcct tcatcatggc gtagaggggc gcgcgctcct catcctgatc tgggtagacg
     3541 cgcttctccc ggatggcagc aacta
//
LOCUS       DAOSCV010000432         3565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16681_length_3565_cov_3.512536, whole genome shotgun sequence.
ACCESSION   DAOSCV010000432 DAOSCV010000000
VERSION     DAOSCV010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_3565_cov_3.512536"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLY56_11040"
     CDS             <1..1222
                     /locus_tag="PLY56_11040"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ22061.1"
                     /translation="GSATRVPAAAQDGKPPTYTALPVRVPMKIDGALTPEEWAGIDPA
                     KAMVLSAGEATARAWAVHAGHSLVIGIDAGEGPTRDEGTEWGQRDGVQVALAAIGQPD
                     QVWVLRGYTDGKFTWGRLAGASTEPEPQPARGLVYRVRAREGGGWTAECRIPFQIIRI
                     RGVGRQPVALHLTVRRAALDRWLTWPAGAGPTLAMGKAGILDWPEVKRWEESILAFEG
                     RDRQNPPPKGALLFVGSSSIVGWDVARFFPDVPTINRGFGGSQLADSVYYFDRIVLPH
                     RPKVVVLYAGDNDIPSGKSPEVVAADFEQFLARVKRDLPGTHLVYIATKPSISRWALR
                     DQYQKANALIREQCEREPLAGYLDIWPAMLGDDGTPRPELFKEDGLHLSEAGYQLWTA
                     LLRDHLKKETAFLP"
     gene            complement(1256..>3565)
                     /locus_tag="PLY56_11045"
     CDS             complement(1256..>3565)
                     /locus_tag="PLY56_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22062.1"
                     /translation="FIGAPYTTSWSSRPEIGPEWQEHSLEFRLGKQPQPLGLWIWIDE
                     GILDLQHLRLVKLSREDLLSELRERYPAPAAKNRAHATRFPLGLPAGWLIGRDYSDGD
                     EVTVVPDRELPGPSGFPALRIAAEKGIEITTAPFLVPPWSGKEHTASLAVRGRAQGKL
                     VAIADGRTLAEAPFTATDEWQRDRVSFTAALFAKAHVVRIEATGTFWVDALQVEEGRE
                     TTAYTPPAPCEVSLALPPSDASPALVQFEDEPAVVLYCVTGEAPGAVLKARVVNVYGV
                     ESPLPDVRLRQGKLHQGRMRYAIFPGRSYGTHRIEAWVEDATGRRLSPETELVVHRLR
                     RPRYWGKDAPNSPFGIHTNATTRHILMAKAVGCNWVRLHDAGTQYIGWAHLEPEKGKW
                     QFFDEPIHRYRKHRLMILGLLSTAPGWATDWGQPTKSYFERYFQPKKMEDWANAVRVI
                     TRRYQGVIDHWEIWNEPWGTLFWIGSYDREKTDKPFRERVIRSKTAAKDYAILQKTAY
                     ETARAEAPGARILGFNTMPGQWTRELLEHGAVESCDIISYHDYSGGILGKPQDAPAKR
                     YEEAIGPFVEKLGRVPRPVWMSEGNVLDCPLENGFYRWTLPYDAPSRVISAADRLARY
                     LISQRALGVEKSFLYTIHGHGAFGVRYPWTALVSADGALHPQGAAHSTLAWLIEDTRF
                     VKCLSPCEGVFAYLFSGRSRAVAALSPDVGHAPYTLPEAPGITCLDLFGNPLSGGTPL
                     GTEVVYLTTSRGFEALEAALKQKPAGARR"
BASE COUNT          556 a         1145 c         1270 g          594 t
ORIGIN      
        1 gggcagcgcg acgcgcgtgc cggctgcggc gcaggatgga aagccgccca cttatactgc
       61 tttgccggtg cgggtgccga tgaagatcga tggcgcgctc actcccgagg agtgggccgg
      121 tatcgatccg gcgaaggcga tggtactttc cgccggggaa gcgacggcgc gcgcctgggc
      181 ggttcacgcg ggccactcgc tcgtgatcgg catcgatgcc ggcgaaggcc cgacgcgcga
      241 cgaaggaacc gagtgggggc agcgcgacgg cgtgcaggtg gcgcttgcgg cgattggcca
      301 gccggaccag gtgtgggttc tgcgtggcta tacggacggg aagttcacct gggggcggct
      361 tgccggcgct agcacagagc cggagccgca gcccgcgagg gggcttgttt accgggtccg
      421 ggcgcgcgag ggcggcggct ggaccgccga gtgccggatc cccttccaga tcattcggat
      481 tcgaggggtc ggccggcagc cggtagcgct ccatctcacc gtgcgccgcg ccgccctcga
      541 tcggtggttg acgtggccgg cgggcgccgg cccgacgctt gcgatgggaa aggcaggaat
      601 cttggattgg cctgaagtga agcggtggga agaatcgatt ctggcgttcg aggggcgtga
      661 ccggcagaat ccgcctccga agggcgcgct cctcttcgtc ggcagctcga gcatcgtcgg
      721 ctgggacgtg gcccggttct tccctgacgt gccaacgatc aaccggggct tcggcgggtc
      781 gcaactcgct gactccgtgt actactttga ccggatcgtg ctgccccacc ggcccaaggt
      841 ggtggtcctc tacgcgggag acaacgacat tccatccgga aagagcccgg aggtcgtcgc
      901 cgcggacttc gagcagttcc tggcccgggt gaagcgcgat ctgccgggca cgcacctggt
      961 ctacatcgcc acgaagccaa gcatttcgcg ctgggcgctg cgagaccagt atcagaaggc
     1021 caacgcgctg atccgcgagc agtgcgagcg cgagcctctt gcgggctacc tcgatatctg
     1081 gccggcgatg ctcggcgacg acggcacgcc gcgccccgag cttttcaagg aggacggcct
     1141 tcacctgagt gaagccggat accagctctg gacggcgctc ctcagagatc acctgaagaa
     1201 ggagacggcg ttcctcccgt gacaggggga gtggcatcta ccggcggcgc gcccctcatc
     1261 gccgggcgcc cgccggtttt tgtttgagag ctgcctccag cgcctcgaac cccctcgacg
     1321 tggtcaggta gaccacctcg gttccgaggg gcgtgccacc actgagcggg ttgccgaaaa
     1381 gatccaggca ggtgatccca ggcgcttcgg gaagcgtgta aggggcatgg cccacgtcgg
     1441 gagagagggc ggcgactgct cgcgaccggc ccgagaagag gtaggcaaag acgccctcgc
     1501 atggcgaaag gcacttcacg aaccgcgtgt cttcaatcag ccaggcgagg gtggagtggg
     1561 ccgcgccttg cgggtggagc gcgccgtcgg cggagacgag cgccgtccac ggatagcgca
     1621 cgccgaaggc gccgtggccg tggatggtgt agaggaagct cttctccacg ccgagcgcgc
     1681 gctggctgat caggtagcgt gccagccggt ccgcggcgct tatcaccctg gacggcgcgt
     1741 cgtagggcag cgtccagcga tagaagccgt tttccagggg gcaatcgagg acattcccct
     1801 cgctcatcca caccggcctg ggcacgcgcc cgagcttctc cacgaagggg ccaatcgcct
     1861 cctcgtagcg cttagccggg gcatcctggg gcttgccaag gatgccgccg gagtagtcgt
     1921 ggtaggagat gatgtcgcag ctctccacgg cgccgtgttc gagcagctcg cgggtccact
     1981 gcccgggcat ggtgttgaac ccgaggatgc gcgcccccgg ggcctcggct ctcgccgtct
     2041 cataggccgt cttctggagg atggcgtaat ccttggccgc ggtcttgctt cggatcacgc
     2101 gctctctaaa cggcttatcg gtcttctccc ggtcgtagct gccgatccag aagagggtgc
     2161 cccacggctc gttccatatc tcccagtgat cgatgacgcc ctggtagcgc cgggtgatga
     2221 cacggacggc gttcgcccaa tcctccatct tcttcggctg gaagtagcgc tcgaagtagc
     2281 tcttcgtggg ctggccccaa tcggtagccc atccgggcgc ggtgctcagc aggccgagaa
     2341 tcatcagccg gtgcttccgg tagcggtgga tcggctcgtc gaagaactgc cacttcccct
     2401 tttccggctc caggtgtgcc cagccgatgt actgagtgcc ggcgtcgtgc agccggaccc
     2461 agttgcagcc caccgccttc gccatcagga tgtgcctggt ggtggcgttc gtgtggatcc
     2521 cgaagggcga gttgggcgcg tccttgcccc agtagcgcgg gcggcgaaga cggtggacca
     2581 ccagctcggt ctccgggctg aggcgccggc ccgtggcgtc ttctacccac gcctcgatcc
     2641 ggtgggtgcc gtaggaccgc ccggggaaga tcgcgtagcg catccttccc tggtgcagct
     2701 tgccttgccg gaggcgcacg tcgggcaggg gactctcgac gccatagacg ttgacgaccc
     2761 tcgccttgag caccgcgccg ggagcctcgc cggtcacgca gtagaggacc acggcgggct
     2821 cgtcctcaaa ctggacgagg gcaggggagg cgtccgatgg gggcagggcg agcgagacct
     2881 cgcacggggc cgggggcgtg taggcggtcg tctcgcgtcc ttcctctact tggagcgcgt
     2941 cgacccagaa ggtgcccgtg gcttcgatcc ggaccacgtg ggctttggcg aagagcgccg
     3001 ccgtgaagct gacacgatcg cgctgccact catccgtggc ggtgaaaggc gcctccgcga
     3061 gcgttcgccc gtcggcgatg gcgacgagtt tgccctgggc gcgtcctctc accgccaggc
     3121 tcgcggtgtg ctccttcccg ctccaggggg gcaccaggaa aggcgcagtc gtgatctcga
     3181 tccccttctc ggcggcaatc cgcagcgcgg ggaagccgga cgggccgggt agctcgcggt
     3241 cggggaccac ggtcacctca tcgccatccg agtaatcgcg cccgatgagc cagccagctg
     3301 gcagcccgag ggggaagcgc gtggcgtgcg cccggttctt cgccgcgggc gccggatagc
     3361 gctcgcgcag ttcggagagc agatcctcgc gcgagagctt caccaggcgc aagtgctgga
     3421 gatcgaggat cccctcgtca atccagatcc agagcccgag tggctggggc tgctttccca
     3481 ggcgaaactc aagcgagtgc tcctgccact cggggccgat ctcgggtcga gaggaccagg
     3541 acgtggtgta gggcgcgccg atgaa
//
LOCUS       DAOSCV010000433         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16751_length_3554_cov_4.026865, whole genome shotgun sequence.
ACCESSION   DAOSCV010000433 DAOSCV010000000
VERSION     DAOSCV010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16751_length_3554_cov_4.026865"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PLY56_11050"
     CDS             complement(<1..1191)
                     /locus_tag="PLY56_11050"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ22063.1"
                     /translation="MKELRTLAGVLSLATLTLFVYGAFAPSGAALASPAIQITHPNPG
                     AIVQGNVTVSVAYRSASGAKITDLVLYVDKAQYASVKLATPATEGTYTFVWNTTGLPS
                     GAHTLTAVAYDAQGNPGDVAVSVYVDPQSSGVDVQAPTVKLTSPASGSVVRGIVTLIA
                     NALDNAKVNMVLFQLNGRLLFATNHPPYRYDLDTTKYPNGPHTIKVYAYDASDNCGES
                     TPVVINVDNPSGRTLPNERPSQLAGSAAVQAKTPAAGTQAAPAPATKPVTAPPSEYQP
                     SVEMARKGIPQLPAVPAASAPRQASARTTPPVVAQPQPSVASRYPDLVVPRSQVTRST
                     GLAAPTSSVTPKATPRPVASAPTAPKAGVTMTDAPRHSSAQAFTIPRQASARTTPPVV
                     AQPQP"
     gene            complement(1325..3166)
                     /locus_tag="PLY56_11055"
     CDS             complement(1325..3166)
                     /locus_tag="PLY56_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22064.1"
                     /translation="MRALGEQIRIALAPAGGIPLFETGRPAEVFLRTQSGLAEPVAVE
                     YTWQLADAHGKRVASGSGTFRVTPGAGSTQTISLPTTRTALYTLQVVARRAGSPQLRE
                     RLVLAVVSPAPKPEQAARSRLGLNLSGPLTASERSLIPLTGAGWARIPIRWGELERKR
                     GCYTLDSELEATLAVLSRAGIRAVASLHPENPLYPADLAADAAWERFCQEIAGRFTGP
                     SGFHAWEIVLPAGPRAGGVSEPSNLLAAAATAIRAIDPEATVIVSAEPSGHADWAGLS
                     GIAACPLTVADLPAVTLFPKPAPVKPGAPRPLYLATLQPEREAALASRDPLSTAGSLA
                     RALLAGLADERIDALFVDPWIDRADSPAPFALAHPDLTPTPALLAYRTVAGLFRSPRL
                     KARIKESFVRGYLFEEGRGVVAALWSPSGEGAIAIKTGAARVTVTDALGAVREYPCHE
                     GMALLPIGPIPCYVRLSGGPVRIEPFLSPLQDPIVCRPGEVSRTRVYLRNPFRRFQPG
                     ALRLVCPPGWRGDLDYPEPGRRARLQAIGVQVMPPAHAEPGAYQLELWIDGGTGTPVQ
                     MQIPLTLLSSEKEAGSAMANAVLTAHTHGNRVLFPRVLNACHHWSKP"
BASE COUNT          626 a         1075 c         1309 g          544 t
ORIGIN      
        1 aggctggggc tgtgcgacca caggaggcgt ggtgcgcgcc gacgcctgcc gcggaatggt
       61 gaacgcctgc gcgctggagt ggcgcggggc gtcggtcatg gtaacgcccg ccttgggcgc
      121 ggtgggcgcg ctcgctactg gtcgaggtgt ggccttaggc gtgacgcttg atgtcggcgc
      181 agccagcccg gtagaacgag tcacctggga gcggggcacc acgagatccg gatagcggct
      241 ggcgacggaa ggctggggct gtgcgaccac aggaggcgtg gtgcgcgccg acgcctgccg
      301 cggagcggaa gccgcaggga cggccgggag ctgcgggatg cccttgcgag ccatctcgac
      361 gctcggctga tactcggaag gcggtgcggt gacaggcttg gtggccggcg ccggggcggc
      421 ttgcgtgccg gcagccgggg tcttggcctg aacggcagcc gagccagcca gctgggaggg
      481 ccgctcgtta ggcagcgttc gaccgctcgg gttatcaacg ttgatgacta cgggagtcga
      541 ctcaccacag ttatccgaag cgtcgtaggc gtagactttg atggtgtgag ggccgttagg
      601 atacttcgtg gtatccaagt cgtagcggta aggaggatgg ttggtggcga agaggaggcg
      661 cccgttcagc tggaagagga ccatattgac cttggcgttg tccagggcgt tcgcgatcaa
      721 ggtgacgata ccccgaacga cgcttcccga ggccgggctt gtcagcttca cggtcggcgc
      781 ctggacgtcc acgccgctcg actgcggatc cacgtaaacg gagacggcca catcaccagg
      841 gttcccctgg gcgtcataag cgacggcggt gagtgtgtgt gcccccgagg gcaacccagt
      901 ggtgttccaa acgaaggtgt acgtcccttc ggtggctggc gtagccagct tcacggacgc
      961 gtattgtgcc ttatcgacgt aaagcaccag gtcggtgatc ttcgcgcccg aggcactccg
     1021 atacgccaca gacaccgtga cgtttccctg aacgattgcc ccaggattcg ggtgggtgat
     1081 ctggatggcg gggctggcga gggctgcgcc gcttggcgcg aatgccccat aaacgaaaag
     1141 ggttaacgtg gcaagtgaga gaacgcccgc taacgttcgt aactccttca caggaccgcc
     1201 tccttcgctg gctacgacta cggtggggaa gctgttgtca cgggctccgg tctgcatgca
     1261 ggtcagagaa ccggcagtgc cgaccacaga cgctgcccca gcatatcata catcggcaac
     1321 ggtgtcaagg cttagaccag tgatggcaag cattcaacac acgcgggaaa agcacccggt
     1381 tgccgtgcgt gtgcgcagtc aatacggcgt tcgccatggc cgagcctgct tccttctcac
     1441 tactcagcaa agtgagaggg atctgcattt ggacaggggt gccggtaccc ccatcgatcc
     1501 acagctccag ttgataggct ccgggctcgg cgtgagcggg gggcatcacc tgaacgccga
     1561 tcgcctggag gcgcgcgcgc cggccaggct ccgggtagtc gaggtcgccg cgccagcctg
     1621 gcgggcagac gagccggagc gcgccgggct ggaaccgtcg gaacggattg cgtagataga
     1681 cgcgggtgcg ggagacctcg ccgggccggc agacgatggg atcctgcaag ggcgaaagga
     1741 acggctcgat cctcaccggg ccgccagaga gacggacgta gcaggggatg gggccgatgg
     1801 ggaggagggc catcccctcg tgacaggggt attcacggac ggcaccgagc gcgtccgtca
     1861 cggtgactcg ggcagcgccg gtcttgatgg caatggctcc ttcacccgat ggactccaga
     1921 gtgcggctac caccccgcgg ccctcctcga aaaggtagcc gcgcacgaag gactctttga
     1981 tgcgtgcctt gaggcgaggg ctccggaaaa gccccgcgac cgtccggtag gcgaggagcg
     2041 ccggcgttgg cgtcaggtcg gggtgcgcca gggcgaaggg agcaggagag tcagcccgat
     2101 cgatccaggg atcgacaaag agcgcgtcga tccgctcgtc ggcgaggccg gccaggagcg
     2161 cgcgagctag agacccggcg gtactcaggg gatcgcgcga ggcgagcgcc gcttccctct
     2221 caggctgcaa cgtggcaaga tagaggggcc gcggagcgcc aggctttaca ggggcgggtt
     2281 tcgggaagag cgttaccgcg gggagatccg ccaccgtcag ggggcaggcc gcgattccag
     2341 aaagaccggc ccaatcagcg tggccggacg gctccgccga gacgatcacg gtcgcctcgg
     2401 gatcaatcgc ccggatggcc gtagccgccg ctgccagcag attcgatggc tcggagactc
     2461 ctcccgcgcg cgggcctgcc gggagaacaa tctcccaggc gtggaacccg gacggccccg
     2521 tgaagcgccc ggcgatctcc tggcaaaacc gctcccaggc cgcatctgcc gccaggtctg
     2581 cggggtaaag cgggttctcc gggtgcaggc tggccacggc gcggatccca gcgcgcgaga
     2641 gaactgcgag cgtggcttcc agctccgaat cgagggtgta gcagccccgc ttcctttcaa
     2701 gctcgcccca gcggatcggg atccgggccc atccggcgcc cgtgaggggg atgagagaac
     2761 gctctgatgc agtgagcggc ccggataggt tcaagccgag ccgggaacgg gcggcctgct
     2821 cgggcttcgg cgcgggcgac acgaccgcga gaaccagacg ctcgcggagc tgcgggctgc
     2881 cggcgcggcg ggccaccacc tggagcgtgt agagagcagt gcgcgtggtc ggcagggaga
     2941 tcgtctgagt ggaaccggcg ccaggcgtga cccggaaggt gcctgatcct gaggccaccc
     3001 gctttccgtg cgcgtcggca agctgccagg tgtactccac cgcgacgggc tccgcgagtc
     3061 cgctctgggt gcgcaggaag acctccgcag gtctgccggt ctcaaagaga ggaatcccac
     3121 cggcgggtgc cagcgcgatg cggatctgct cacccagtgc gcgcatttcc atggaggata
     3181 tctcttgccg gaagccctcg gcctccatct gaaggggttc gggatccggc gctggcatcg
     3241 gccaggaggt acccgcgctt gctggatgat tcaagagaag cgcgaggagc gccaggcagg
     3301 gagtgaggat ccgccaccgc cggacgcggt ggtgtgccgg aggggttacg acgccaccgg
     3361 aagaggggtg ggacctgatg agtgcctggg atctgcgtgt gtgccggccc tgcatggagg
     3421 cgtgttcggc atctggccgg cgccatcctt cccgcacacc tgcaggatca gaccgctaga
     3481 ggcggggacg cgggggcgcc gaactccgcg gctgcacgcg acgataccgc ggatgaagat
     3541 ggtcggccaa gaca
//
LOCUS       DAOSCV010000434         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16807_length_3546_cov_2.896878, whole genome shotgun sequence.
ACCESSION   DAOSCV010000434 DAOSCV010000000
VERSION     DAOSCV010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16807_length_3546_cov_2.896878"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..228
                     /locus_tag="PLY56_11060"
                     /pseudo
     CDS             67..228
                     /locus_tag="PLY56_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013510146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(1270..2229)
                     /locus_tag="PLY56_11065"
                     /pseudo
     CDS             complement(1270..2229)
                     /locus_tag="PLY56_11065"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
BASE COUNT          611 a         1178 c         1212 g          545 t
ORIGIN      
        1 ccgtctaaga tcgattgatg gcaaggaagg tggctacgct tacgatgggt tcggaaggcg
       61 agtaagcgct gtggttggta ccatgaatcc aagattcagg accttcttcc acgagaagtc
      121 gggcaagacg ccgttcaagc catgctcaaa gaaatcgctc gatcgggaga tggttcgttc
      181 ctcgctgtcc tgaagacctt tggcaaccgc cagccggtgg cactgctcgg ccaactcgcg
      241 cgagcgcctg gcgagcgctc ccaggtcata ccgccccgtc tgaggcgagg gggtcaggcc
      301 agggatcgcc tggaggggca gatgcaactc gcgcgctgct ccgggagcca gccggacgga
      361 accggcttca ggtgcgagcg cggcgagcac gccggtcgcc tcgcgagcga cgacctgcag
      421 gccctcgacc ccgcgaagct ggagcgcctg tgcgccgtcc acggtcacga ccaggccacc
      481 gcgcgaggca tcggccacac gcgcgcgaac tgggggctcg attgtcgccg ggtggcgcaa
      541 cgccaggagg aaatcctggt cggcccgcgt gccggtcgcg gtgagggtac catcgggcgc
      601 aatctccagc tcggcgccct cggcatacca caggctaccg ggctcaacgc gcgcttgcgg
      661 gaaagttcca tccttgccct tgcgcaggaa gaggaacgga agatccgccc gcgcgggggc
      721 cgcggacggt tccttgccgc caaactccag caggagcacg ctacgcgctg gcagctgggc
      781 ccgcagggtt ccatccggcc ccatctccac ggtgccggtc ctctcttgcc ctgcccagag
      841 ctggcgcggc cggaccgagc cggacggtac accccacgcc accggatcgc agcgcagcga
      901 cacgggctgg acctcgccgg tccagttggt gaggagcagg ccgacgcggc catccggcgc
      961 cttccagaga gagtggcgga cgacgggcat cgagaccgtc gcgctgtgcg tgaagccgat
     1021 ggacatcttc cgttccggca cgtcgagcgc cggcgggcgg agccacttcc cttccaggag
     1081 gaacgctctc ccggcgtgga aagtgtgcgt cgtcgcgcgc aggtaggcgg cactctcggg
     1141 cttctcgcca atcttcgggg gggcgttttc ggaaagcgtc ggcacggcgc cccacgccag
     1201 gtgctccgcc atcagggcgc agaaggtatc ggtgtcgcgg cttagcgcgc agtcactgcc
     1261 atactgaatg gcatagtcat ggtagaccgc ggtgaagagc gggcagagct tgacgagagg
     1321 ggtgtagccg ccgcgcgtta cgtcaagcgt caggaagccg tcgaagccat ccaggtaggc
     1381 ctcacagatc tcctcggtgg tgaagacggc ttccggcttg cgctcgcgga tccggtcgcg
     1441 gaggcggcgc atcaagacgc ggttcccctc gccccagtag ttcccgccgt tgagcgggtg
     1501 cccgtgcttc ttgtcgaagc agggccgcgc gcgccccgcg gagaggacgt cgaggtagac
     1561 gccatccagg ccgtaatccc atacctgctt gctgacgaaa tcgaacacct tctcctgcca
     1621 ctgccgggtg aaggggcaca tccaggcaaa ggtgttctcc gcaatcggcc agaggtagaa
     1681 gtcgcccgcc tcggtcttgg cggctgcggc cgcgccgttc tccagccgcc agctttcagt
     1741 atccatatcc cagatgctcc cctgggtgta ggggatcaca tgcgcaccga gtgcctgcat
     1801 ggcgcgcacg ccctcgaggt aaccaggctt cgcggggagc atctccggat agttatcatc
     1861 gaacttcgcc accgggtagc ggtagtaatg cacagcggtg ggcacgcgca ggtagtcgaa
     1921 gtggtcagcc agctcgccga gcaccttgcc cggctcgttg tagtacttca gccagagcgc
     1981 cgcctccttg aaccactcgg ggatcgaccg gcgcgtggcg atggggcctt cggcgcacca
     2041 cttctggcgc agcgcccact cgcggtagac ctgggccgag tcgtaccagt cgccgtcgaa
     2101 aacgccgacc atggcaggat aggggagctc ccactggcgt ggcttcggca agggatccgg
     2161 cgcggggagg atgagcgaaa gctcgcccgt cgcggccttc gtatccgcgc tccattggat
     2221 gtgcttcatc cagcaatcgg gatcggcggc ggccacgtag agcccggctc ccccgccgta
     2281 gtaggacgca tactgcatcg aaccggggca gggataggtg accgtgtagc tattcagccg
     2341 gcgcaaggga tccgtcgcca gccggcccca gtagaccgga agggtgagcg cgtcgtcctc
     2401 agcgaccttg cggatcccgg caacccgggg gaactccacc tcgcgcagcg tcagcttcgg
     2461 gtcggtccaa tcgagagcga gccgccactc cgagagcttc gcatccggac cgagcgaaat
     2521 ggtgacgcga acgcgggcgg caccctgacc ggcgcggact ccctcccaca ccaacgcgag
     2581 ccggccctgc gcgggttcgg cccgccgggt ggccggggca tccgcggcga tgacgaggtc
     2641 gttccccgcg ccatcgcgga acgtgagccg ccacaacggc ccggtgcgat cacccagaaa
     2701 cgcggtttgc cgtccgaggt gcgcgagctc cgtcagcgcg aagccccgtg cctcatcgaa
     2761 gctgaggcgg agatgcttgt tctcgaggcg caccgccgcg cccgccgggg ccaccacgcc
     2821 gatggcgcag agaagaccta cacaaaagag aaggatgcgg gtacgccccc agccgcgggg
     2881 ccgcccatgg atcggctgtt ctctcaccta actacctccc tggaacgcgc tgctccctcc
     2941 cccatagagg aaggggctcc tgtccagtat agcaaggatt gctcccgccc gcaagggagg
     3001 cacccgcggt gggggttttc gggataagcc cgcacgcgcg gacctcgaag ctccgcgctg
     3061 cttcctggca aagtcccttc ggggcttccc ccccagcccc tgccgttctc aggcacccgg
     3121 gtgtgttgcc aaccccagcc cggcagggct tcgctcgcaa gcagcgcggt gatttatcgc
     3181 cgcacgcctg gttgcgcgtc ccccgcgcgt gggcgagggc gggggccccg cgcttccatg
     3241 tttgggctgg tctccattcc gccgccggac ccgattgggt cgcgccatgc tcgcgccgtt
     3301 tcacgcgcgg tgtcgtgtgg aaagagaggc agcggagaag cattgcgctg ccgtgcagtc
     3361 tcgacgccac ggccggctgc ggtgcagaga gggaaggatc aatgcgcaag aaggaggaaa
     3421 agaagaggct tcacgagcag atgcgcgaag cgggcgagat gctgggccag gctcggaagg
     3481 aagagctggg ccacgaaggc tacgaagagt tgggccataa ggggggccag cgcgtccgcg
     3541 agctga
//
LOCUS       DAOSCV010000435         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16875_length_3535_cov_4.253736, whole genome shotgun sequence.
ACCESSION   DAOSCV010000435 DAOSCV010000000
VERSION     DAOSCV010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16875_length_3535_cov_4.253736"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLY56_11070"
     CDS             complement(<1..712)
                     /locus_tag="PLY56_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ22065.1"
                     /translation="MGRAAFYNGKGQIRLEEVPERVPGPGEAMVRVRACGVCGSDLHS
                     FQNRWPQPPVVPGHEVAGTVEAVGDGVSNVKVGDAVALEPFVSCGECRPCRSGQYNVC
                     TRHEFISWHRDGGFADQMVVPARCLLALPEGPAQQYGALVEPLAVAVHGLRRAPLMGG
                     DTVVVLGAGTIGLLAAAAARALGAGRVLLTAKYPHQAAAAEQMGIRDVIRLGEGNPAE
                     AILERAGEDAVDVVLESVG"
     gene            complement(844..1608)
                     /locus_tag="PLY56_11075"
     CDS             complement(844..1608)
                     /locus_tag="PLY56_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ22066.1"
                     /translation="MLTLSACIEMIFRDLPFPDRIAKVAECGLPACEFWRWVDKDLDA
                     IVKAKEAAGIAIACCSCDTRGPLVADGSAATFVEGLKASCEAAHRLGTRTLIVTTGQE
                     IPGKPRSEQHANIVTALKEGAPIAEREGITLVLEPLNVLVNHKGYYLSTSAEGFEILR
                     EVGSPNVKLLFDIYHQQITEGNLINNIRDGIDLIGHFHVADNPGRNEPGTGEINYRNV
                     FKAIEAAGYQGYVGLEYHPLADPAETLKNTLALARG"
     gene            1789..2154
                     /gene="sdhC"
                     /locus_tag="PLY56_11080"
     CDS             1789..2154
                     /gene="sdhC"
                     /locus_tag="PLY56_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOJ22067.1"
                     /translation="MIREIFTYRGKEGYWLWVLHRATGVGIFLFLAIHIVDTLLVFWP
                     NLYDAVTGLYRHPLFRVGEILLVGMVLFHAVNGLRILVLDLWEASTKHRRELFYATLA
                     LLAVLLIPALLLMVREIIL"
     gene            2168..2590
                     /locus_tag="PLY56_11085"
     CDS             2168..2590
                     /locus_tag="PLY56_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HOJ22068.1"
                     /translation="MALGKRQIAEQPRPTGNFELFSWYFMRVSGVVLLFLILGHLAVM
                     HLFGSVEDVSAAFVAERLANPLWRVYDLTMVVLALLHGGNGVRWILDDYVHSNRWRLI
                     AFSTLYTALFVLLVVGSLILLTFQVPGSPAAGSFSAMR"
     gene            complement(2574..>3535)
                     /locus_tag="PLY56_11090"
     CDS             complement(2574..>3535)
                     /locus_tag="PLY56_11090"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ22069.1"
                     /translation="SVFVVADNCTDATAAVARARGARVYERADREQRGKGYALRWLLE
                     RIAETGEKYDAFVIFDADSRVDPAFLRVMDETLAREDEVLQAHYSVLNPGEGIGAAIR
                     FAALALVNYLRPLGKNALGCSTGLKGNGMCFPAAVIRRHGWAAYSLAEDAEHGVLLLL
                     DGIRTRFVPEACVWAEMPPGVRESRSQNLRWEAGRLLVVRKWVPQLLRHAVRHRDLVA
                     LEAAIDLLVPPLSALVAGTLLLALMALLPGLGGTWWVPTGIGVALGGHVLVGLIRIKA
                     PRSVWRSLLYAPGYCAWKLALYASATARAPSEWVRTRRFSASR"
BASE COUNT          580 a         1218 c         1140 g          597 t
ORIGIN      
        1 ccccgacgct ctccaggacg acatcgacgg cgtcctcgcc ggcgcgctcg aggatcgcct
       61 cggcgggatt gccctctccc aggcggatca cgtcccggat gcccatctgc tcggcggcag
      121 ccgcctgatg cgggtacttc gccgtcagca gaacgcgccc ggcacccagc gcccgtgccg
      181 ccgcggcggc caggagacca atggttccag cgccaaggac caccacggta tcgccgccca
      241 tcagcggggc gcggcgcagg ccgtggaccg ccacagcgag cggctccacg agcgcgccat
      301 actgctgcgc cgggccctct gggagcgcca gcaggcagcg cgccggcacg accatctgat
      361 ccgcgaagcc accatcgcgg tgccaggaga tgaactcgtg gcgcgtgcag acgttatact
      421 gccccgaccg gcatggtcgg cactcgccgc aagagacgaa cggctccagc gcgaccgcgt
      481 cgcccacctt cacgttggag acgccatcgc ccaccgcctc gaccgtgccg gcgacctcat
      541 gcccgggcac aaccggaggc tgtggccagc gattctggaa cgaatggagg tcgctgccgc
      601 acacgccgca ggcgcgcacg cgcaccatcg cctcgcccgg tcccggaaca cgctccggca
      661 cctcctccag gcggatctgg ccttttccgt tgtagaacgc agctctaccc atcagatcac
      721 acccccagga tcgtattcga cactccccat tcaaaccccg ccagcgaggc gccagcacgt
      781 gcggtggccc acgcgcctcg gcccgcgaga gcccggcgcg gcccccgcgg gccagccaca
      841 gctttagccc ctggcgagag cgagggtgtt cttgagcgtc tcggccggat cggcgagggg
      901 gtgatactcc aggccgacgt agccctggta gcccgcagcc tcaatcgcct tgaagacgtt
      961 gcgatagttg atctcgccgg tgcccggctc gttgcgcccg gggttgtcgg ccacgtggaa
     1021 gtgaccgatg aggtcgatgc cgtcgcggat gttattgatc aggttcccct cggtgatctg
     1081 ctggtggtag atgtcaaaga ggagcttcac gttcgggcta cccacctcgc ggaggatctc
     1141 gaagccctcg gcggaggtgg agagatagta gcccttgtgg ttcaccagga cgttgagcgg
     1201 ctcgagaacc agcgtgatcc cctcgcgctc ggcgattggc gcaccctcct tcagggcggt
     1261 gacgatgttg gcgtgttgct cggagcgagg cttgccgggg atctcctggc cagtggtgac
     1321 aatcaacgtc cgcgtgccca ggcggtgcgc ggcctcgcac gacgccttca ggccctcgac
     1381 gaaagtcgcg gcgctgccgt cggccaccag cgggcctcgg gtgtcacacg agcagcacgc
     1441 gatggcgatc ccagcggctt ccttcgcctt gacgatggcg tcgaggtcct tatcgaccca
     1501 gcgccagaac tcgcacgcag gcaggccgca ctcagcgact ttagcgatcc ggtctgggaa
     1561 ggggagatct cggaagatca tctcgatgca ggcagagagc gtcagcatgg cgatccctct
     1621 tttccgaaga aacggtggca ggcacgcacc cccgcgcggg gcaggagcgt gtcgcttgcg
     1681 atcaggattc cgaggaggcg ccgatcttcc tctttgccga tccgctgttg acaccccccg
     1741 gtgcgtctgc tatcatagca gacggttgtt gttgggagga gcgacgggat gatccgcgag
     1801 atcttcactt atcgggggaa agagggctac tggttgtggg tgctccaccg cgccacgggc
     1861 gtgggcatct tcctctttct ggcaatccat atcgtggaca ccctgctcgt cttctggcct
     1921 aacctgtatg acgcggtgac cggcctctac cggcacccgc tcttccgcgt gggcgagatt
     1981 ctcctggtcg gcatggtcct cttccacgcc gtcaacggcc tgcgcatcct cgtgctggac
     2041 ctctgggagg catcgacgaa gcaccggcga gagctgttct acgcgacgct ggcgctcctg
     2101 gcggtgctcc tcattccagc gcttcttctg atggtgcgag agattatcct gtgagtggga
     2161 gggtgtgatg gcgctaggca aacggcagat cgcggaacag cctcggccta cggggaactt
     2221 cgagctcttc tcgtggtact tcatgcgcgt ctcgggagtg gtgctcctct tcctcatcct
     2281 gggtcatctg gcggtgatgc atctcttcgg gagcgtggag gatgtgagcg ccgccttcgt
     2341 agccgagcgc ctggccaatc ccctctggcg cgtgtatgac ctgacgatgg tagtcctagc
     2401 gctgctccac ggcggcaacg gcgtgcgctg gatcctcgat gactatgtgc atagcaaccg
     2461 gtggcgcctg atcgccttct ctacgctcta tacggccctc ttcgtgctcc tggtggtggg
     2521 ctcgctgatc cttctcacct tccaggtccc tggctcgccc gcagctggta gcttcagcgc
     2581 gatgcgctaa accgccgggt ccgcacccat tccgagggtg cgcgcgccgt cgcggatgca
     2641 tacagcgcca gcttccaggc gcagtagccg ggcgcgtaga ggagcgaccg ccagacgctg
     2701 cgcggtgcct tgatccgaat cagcccaacg aggacgtggc cgccgagagc gactccaatg
     2761 ccggtcggca cccaccaggt cccgcccagc cccggcagca gtgccatgag ggcgagcagc
     2821 agcgtgccgg caaccagggc cgaaagcgga ggcaccagga ggtcgatagc cgcctccagc
     2881 gcgaccaggt cgcgatgccg cacggcatgt cggaggagct gaggaaccca cttgcgcacg
     2941 acaagcagcc ggcctgcctc ccaacggagg ttttgcgatc ggctctccct gaccccgggc
     3001 ggcatctcgg cccacacgca cgcttccgga acgaagcgcg tgcggatccc atcgaggagc
     3061 aggagcacgc catgctcggc atcctctgcc agcgaatagg cagcccaacc gtgccggcga
     3121 atcacggcgg ccgggaagca catgccgttc cccttcagcc ctgtcgaaca accgagggcg
     3181 ttcttaccca ggggccgcag gtagttgacc agcgccagcg cggcaaaacg gatggcggcg
     3241 ccgatcccct cgcccgggtt gagaacgctg tagtgcgcct ggagcacctc atcctcgcgg
     3301 gccagcgtct catccatcac ccggaggaag gcgggatcga cgcgcgagtc ggcgtcgaag
     3361 atcacgaagg cgtcgtactt ctcgcccgtc tccgcgatcc gctccaggag ccagcgcagc
     3421 gcgtagccct tcccccgttg ttcccgatcc gcgcgctcat aaacgcgagc gccgcgcgcg
     3481 cgcgccaccg cagccgtggc atcggtgcag ttgtcggcga cgacgaaaac cgagt
//
LOCUS       DAOSCV010000436         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16902_length_3533_cov_3.373778, whole genome shotgun sequence.
ACCESSION   DAOSCV010000436 DAOSCV010000000
VERSION     DAOSCV010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16902_length_3533_cov_3.373778"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLY56_11095"
     CDS             <1..393
                     /locus_tag="PLY56_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22070.1"
                     /translation="LAKGRRAAVTAAEALWPSTRSDTSPGFMMRGNAVVGGARCMAET
                     QPVLSAAAYSLTALRAVRRHEIVHGPQADSVVGTVELAPLPDQARSTLPIGFPAPNLS
                     TGVRYILRRATPEPWRVGSWAVVLGPRE"
     gene            complement(400..1092)
                     /locus_tag="PLY56_11100"
     CDS             complement(400..1092)
                     /locus_tag="PLY56_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22071.1"
                     /translation="MHRRSGFTLIELLVVIAIIAILAAILFPVFSRARENARKSTCQS
                     NLKQIGMAMMQYVQDYDETLPQHYFRSPSDVVVSSIITLLHPYVKNVGVWDCPSADMK
                     CKRSGSDPIVLGDRSYGWNYYIFPLSSPARLARLTSPADTVCAADCTGDSWGPGRLYY
                     PSTGARTDPIDGSQNNSWWDTDAHGGVRPGFNFVVRHNQMGNVLFMDGHVKAMSYWAL
                     YSNGQDTLFDWD"
     gene            1468..2520
                     /locus_tag="PLY56_11105"
     CDS             1468..2520
                     /locus_tag="PLY56_11105"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22072.1"
                     /translation="MSKTRVGIIGCGGMARSHASRFNMILDQVEITATVDVVRERAQA
                     VADLLPGEVAVAEDYHEILDKVDAVLLVLPHHLHHPVTLECLAAGKHVLVEKPMANSE
                     QECLEMIEAAKRADRVLMVAYCMRFHPLVVKMKELIDAKTYGDVFQLSIWTEQLTRYP
                     EDHWASSAKTLGGGQLFSHGCHYIDLLLWMLGKPVWGTHFGTNTGTPWMEREGTSNVT
                     IEFEGGVMGYHMGTWGARGSRLKYSFHAHCTEGMIEADISHGRLIAHSKSVIHDPANH
                     DAAQAEEVLLEIPHAKPTEVEMAHFIECIRTGKKPLTDPVSSLEGLKVIWKLYEAEEK
                     KTLADLRGMGLGTLKL"
     gene            2636..>3533
                     /locus_tag="PLY56_11110"
     CDS             2636..>3533
                     /locus_tag="PLY56_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ22073.1"
                     /translation="MAGNEKKTPEARLQISGIYPHLAVFSHEGEVGIGAVVPWAGKLW
                     MITYPPHHRTGGPDKLYEIDPDLNVTIRPESVGGTHACRMIHRESNQLIIGPYFIDAR
                     GGVRAADPHVLVGRMTAVMRHLKDPANKVYFFDMEGPIYEVDVHTLAVKLLFKKPVPG
                     WHGKGGYTAQGRVVIANNGERQAIDGPFHFLAEPETTTPEDAGILAEWDGERWRVIER
                     KQYCEVTGPGGICGAPDDQSPLWASGWDKRSVILKLLDGGQWSTFRIPKASHCYDPIH
                     GWYTEWPRIREVGGGKWMMTMHG"
BASE COUNT          648 a         1073 c         1205 g          607 t
ORIGIN      
        1 ctggcgaagg ggagacgcgc cgcagtgacc gcggcagagg cgctctggcc atctactcgc
       61 agcgacacct ccccgggctt catgatgcgg ggcaacgcgg tggtcggcgg ggcccgctgc
      121 atggctgaga cgcagccggt gctatccgcc gccgcgtatt ccttgaccgc gttgagggca
      181 gtgcgcaggc acgagatcgt ccatggcccg caggctgata gtgtggtcgg gacggtggaa
      241 ctcgccccac tgcccgacca ggcacgatcc acactgccca tcgggtttcc cgcacccaac
      301 ctgtctacgg gcgtgcgcta catcttgcgg cgcgcgacgc ccgagccgtg gcgcgttgga
      361 tcgtgggccg tggtgcttgg cccaagggaa tagcgcgcgt caatcccaat cgaagagtgt
      421 gtcctggccg ttggaataga gtgcccagta actcatcgcc ttcacatgcc catccatgaa
      481 gaggacgttc cccatctggt tgtgccggac gacgaagttg aagccaggcc ggacgccccc
      541 gtgagcatcc gtgtcccacc aggagttgtt ctgcgagcca tcgattggat cagtgcgagc
      601 gccggtgctg gggtagtaga ggcggcccgg gccccaggag tcgccggtgc agtccgcggc
      661 gcagacggta tccgcgggtg aggtgagtct tgccagcctc gcgggggagg agagagggaa
      721 gatgtagtag ttccacccgt agctgcggtc gccgagcacg ataggatcgc tccccgagcg
      781 tttgcacttc atgtcggcgc tgggacagtc ccacacgccc acgttcttga cgtaggggtg
      841 caggagtgtg atgatgctgg agacaacgac gtccgatggc gatctgaagt agtgctgcgg
      901 cagggtttcg tcatagtctt gcacatactg catcatcgcc atgccaatct gcttgaggtt
      961 ggattgacag gtggatttgc gcgcgttctc ccgcgcgcga gagaagaccg ggaagaggat
     1021 cgccgccaag atggctatga tagcgatcac taccaaaagc tcgatcaggg taaaaccgct
     1081 acgcctgtgc attgctgacc tccaatcggt gtcacgatgt tcctcagcgt atccatcatg
     1141 gtttggcctg ctattccgtg cgcccctcgg gcattcctgc taaggtgacc cctcgttctc
     1201 cgcggtccgg ctagcgcatc ggcaatcggg tgtgctgcga atgccactcc ggagcgccat
     1261 cctaccggtg gctcggcaac cgcgcgtctg cgcctgcgaa gaggcccagt accggcctgc
     1321 ccgatgcccc ggggtgccgg aaacgggctt gttgtgacga tccgttacag gggttttctg
     1381 gatgtatacc gaatcagggg ggggactggt ctgtgccgcg cgggctgaag ccgttatctg
     1441 gcgcggcgca gttggaggaa acgtttcatg tcgaagacac gggtaggtat cataggttgt
     1501 ggtgggatgg cgcggagcca cgccagccga ttcaacatga ttctcgacca ggtggagatc
     1561 acggcgaccg tggacgtcgt gcgcgagcgc gcgcaagcgg tggcggatct gttgcccggc
     1621 gaggtggcgg ttgcggaaga ctaccatgag atcctggaca aggtggatgc cgtcctcctg
     1681 gtgctgccac accacctgca ccatccggtg acgctggagt gcctggcggc cggcaagcac
     1741 gtcctcgtcg agaagccgat ggcgaactcc gagcaagagt gcctggagat gatcgaggcc
     1801 gccaagcggg cggatcgcgt gctgatggtg gcctactgca tgcgcttcca tccgctggtg
     1861 gtgaagatga aggagctgat cgacgccaag acctacggcg acgtcttcca gctctcgatc
     1921 tggaccgagc agctgactcg ctatccggag gaccactggg cgagcagcgc gaagacgctg
     1981 ggcggtggtc agctcttcag ccacggctgc cattacatcg atctgctcct ctggatgctg
     2041 ggcaagccgg tttggggtac ccacttcggc acgaacaccg gaacgccctg gatggagcgc
     2101 gagggcacga gcaacgtgac catcgagttc gaggggggcg tgatgggtta ccacatgggc
     2161 acctggggcg cccgcggcag ccggctgaag tactccttcc acgcccactg caccgagggg
     2221 atgatcgagg cggacattag ccacggccgg ctgatcgccc actcgaagag cgtgatccac
     2281 gatccggcca accacgacgc agcccaggct gaggaagtgc tcctggagat cccccacgcc
     2341 aagccgaccg aggtggagat ggcccacttc atcgagtgca tccgcaccgg gaagaagccc
     2401 ctcaccgacc cggtgagctc cctggaaggc ctgaaggtga tctggaagct gtatgaggcg
     2461 gaggagaaga agacactcgc cgatctacgc ggcatgggtc tcggcactct gaagctgtga
     2521 gcgaatcact ggggcgccgt ggtgatgctg gcgccccgtc ttcttctttt cgcgcggggg
     2581 tgagcaccgg ccccgcgtgc cccgcgcacg gctgcaaacg ctcggggaga tgagcatggc
     2641 agggaacgag aagaagacac cggaggcgcg cctccagatc agtggcatct atccccacct
     2701 ggcggtcttc agccacgagg gcgaggtggg gatcggcgcg gtggtgccct gggcgggaaa
     2761 gctgtggatg atcacctacc cgccgcatca tcgcactggc gggccggaca agctctatga
     2821 gatcgacccg gatctcaacg tcaccatccg cccggaaagc gtcggtggca cgcacgcctg
     2881 ccgcatgatc caccgcgagt cgaaccagct gatcatcggg ccctacttca tcgatgcccg
     2941 gggaggagtg cgcgcggccg atccgcacgt gctcgtgggc cggatgaccg ccgtcatgcg
     3001 ccacctcaag gacccggcga acaaggtcta cttcttcgac atggaagggc cgatctacga
     3061 ggtggacgtg cacaccctgg cggtgaagct cctctttaag aagccggtgc ccggctggca
     3121 tggcaaggga ggctacaccg cgcaggggcg cgtggtcatt gccaacaacg gcgagcgcca
     3181 ggccatcgac gggccgttcc acttcctcgc cgagcccgag acgaccacgc cggaggatgc
     3241 cggtatcctc gccgagtggg atggcgagcg gtggcgcgtc atcgagcgga agcagtactg
     3301 cgaggtgacg ggaccgggcg ggatctgtgg cgctcccgat gaccaaagcc ccctctgggc
     3361 ttccgggtgg gacaagcgct cggtgattct gaagctcctg gatggcgggc agtggagtac
     3421 cttccgcatc ccgaaggcaa gccactgcta cgacccgatc cacggctggt acaccgagtg
     3481 gccgcgcatc cgcgaggtgg gcggcggcaa gtggatgatg acgatgcacg gca
//
LOCUS       DAOSCV010000437         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16915_length_3532_cov_2.828300, whole genome shotgun sequence.
ACCESSION   DAOSCV010000437 DAOSCV010000000
VERSION     DAOSCV010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16915_length_3532_cov_2.828300"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLY56_11115"
     CDS             <1..734
                     /locus_tag="PLY56_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOJ22074.1"
                     /translation="SSIQGESQLIGHRQIFLRFLGCNLRCDYCDTPETLSSSQSLPLC
                     RIERTPGCGDFEYRENPLSLGDVREAVARLNQVCHHSVALTGGEPLLQADSIIALLPA
                     LRDLGLRVYLETNGSLPDRLDWVLEGIDIIGMDVKLPSVAGESAENLLERHQEFLARA
                     RCKELFVKIVVGDATPDAELEAAVRMVAEVDPALPITLQPVTPRGPAVYPPSGSRLLE
                     MQALAQRHADTVRVIPQTHHLMGLL"
     gene            764..1147
                     /gene="hisI"
                     /locus_tag="PLY56_11120"
     CDS             764..1147
                     /gene="hisI"
                     /locus_tag="PLY56_11120"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOJ22075.1"
                     /translation="MDLFDVVKFDSNGLVTAVVQDDASGEVLMVAYMNREALEQTLKT
                     GRVTFWSRSRREMWVKGLTSGHTQTLKSIAVDCDGDALLVRVDQVGGACHEGYRTCFF
                     RRAGADRQLEITGERLFDPKQVYRK"
     gene            1158..2402
                     /locus_tag="PLY56_11125"
     CDS             1158..2402
                     /locus_tag="PLY56_11125"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ22076.1"
                     /translation="MLRRALLILAAVAVVVGSGLAGVWFARSPARLGRSSPLTLSAIV
                     GADAIVVQAGTTGKVRLVAVKEGDMVQAGQLIAWLEAPELRESVRAAEDAVRDAQRRL
                     DALRSGRPSRERLKLARAEAEATAAQRALEQAEELSRQVPDLTERVATARRALDEATL
                     QLASAEADANQGNVVSPSTADTGGGVALESSGDGENTGESAVETPAANPVPDFVREAR
                     RRKEAAARALARAEEQLRRAERAARQLAALADRVSATAVAVDEARKELSERIAAEGRG
                     GSEEERLRREVREAQAIAAQARARLREAVVVAAAGGLVEHVAVSAGQELAEGEAIATL
                     RQLNPVWAEVTLAPNAVNRVHRKQKVTLSIPSSERIAAVGEIASMAPRSTHRESPWTV
                     KVVFPNPQHQLLPGAEVQLTFR"
     gene            2444..3142
                     /locus_tag="PLY56_11130"
     CDS             2444..3142
                     /locus_tag="PLY56_11130"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ22077.1"
                     /translation="MESFTGAVAFLVKGGPIMVPLLAASFLAVAVTIERAVILARASR
                     DSGPLMRRVREALQRGAFDEAVRACEEANTPVGRVLAAGLRARHLPPERLEKVLEEEA
                     LSELPALNRRLVVLDTVITIAPLLGLLGTVTGMIRSFGIMAVSGIDRPHGITGGVAEA
                     LIATAVGLAIAITTLIAYNWFTEKVRDITSQMEIRATQLINLLAEARAEPWSGRPGTD
                     GPCYARDARVEAAE"
     gene            3139..>3532
                     /locus_tag="PLY56_11135"
     CDS             3139..>3532
                     /locus_tag="PLY56_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ22078.1"
                     /translation="MRLPRHEQKKARIEIIPMIDTIFFLLVFFMLASLAMTRMTGVKV
                     NLPKATSGERTTAAKFVVTLTATGRYYVEKEHAADFEALRAMVERRVRENPEAVAVIN
                     ADKSVRHGEVIALMDLVKSAGVARMTVAT"
BASE COUNT          591 a         1113 c         1242 g          586 t
ORIGIN      
        1 tcagcagcat tcagggggag tcgcagctca tcgggcaccg ccagatcttc ctgcgcttcc
       61 tcgggtgcaa tctccgctgc gactattgcg atacccccga gacactctct tccagtcaaa
      121 gcctcccgct atgccggatc gagcgcacgc ccggatgcgg tgacttcgag tatcgcgaga
      181 atccgctctc gctgggggac gtgcgcgaag cggtggcccg cctgaatcag gtctgccatc
      241 actcggttgc cctcacaggg ggcgagccgc tcctccaggc ggattccatc atcgcgctgt
      301 tgcccgcctt gcgcgatcta ggcctgcgcg tttacctcga gacgaatgga tcccttccag
      361 accgtctcga ctgggtgctc gaaggcatcg acattattgg catggacgtg aagctgccct
      421 cagttgctgg ggagagcgca gagaaccttc tagagcgaca ccaggagttt ctggcgcgtg
      481 cccgctgcaa agagctcttt gtgaagatcg tggtcggcga tgcgacgccg gatgccgagc
      541 tggaagcggc agtgcgcatg gtcgccgaag tggaccccgc cctcccaatt accttacaac
      601 ctgtcactcc ccggggaccg gcggtctacc ccccttcggg ttcgcggttg ttggagatgc
      661 aggcgctggc ccagcgccat gccgatacgg tgcgcgtgat cccgcagacg catcatctga
      721 tggggcttct ctgacgcgct gagcgctgga tgaaaggtac aacatggatc tctttgatgt
      781 agtcaagttt gatagtaacg gcctggtgac ggccgtcgtg caggacgatg ccagcggcga
      841 ggtgctgatg gtcgcctaca tgaatcggga agccctggag cagacgctca agacggggcg
      901 cgtcaccttc tggagccgct cgcggcgcga gatgtgggtc aagggcctga cctccggaca
      961 cacgcagaca ctcaagagta tcgcagtaga ctgcgacgga gatgcgctcc tggttcgcgt
     1021 ggatcaggtg ggtggggcgt gccacgaggg ctaccgcacc tgcttcttcc ggcgcgcggg
     1081 cgccgaccgc cagctggaga tcacgggcga gcgcctcttc gatccgaagc aggtttaccg
     1141 aaagtagcgg ggtgccgatg ttgcggcgag cgttgctcat cttggcggcg gtggccgtgg
     1201 tggtggggtc cggactggcg ggcgtctggt ttgcccggtc tcccgctcgc ctggggcgct
     1261 cgtcgccgct caccctgagc gccatcgtgg gcgccgatgc catcgtggtc caggcaggga
     1321 ccacgggcaa ggtgcgtctg gtcgccgtga aagagggcga catggtccag gcaggccagc
     1381 tgattgcctg gctggaagcg cccgagctac gcgaatccgt gcgggcggca gaggacgcgg
     1441 ttcgcgatgc gcagaggcgc ctggatgcgc ttcgttctgg aaggccaagc cgcgagcggt
     1501 tgaagctggc gcgtgccgag gcagaggcta ctgcggcgca gcgcgccctt gagcaagcgg
     1561 aagagctcag ccgtcaggtt cccgacctca ccgaacgcgt ggcaactgcc cggagggcgc
     1621 ttgatgaggc gacgctgcaa ctcgcgtccg ccgaggcaga cgcaaaccag gggaatgttg
     1681 tctctccctc tactgccgac acggggggcg gggtagccct ggaaagcagc ggagacggtg
     1741 aaaacacggg ggagagcgcg gtggagaccc cggcggccaa cccggtgccg gatttcgtga
     1801 gggaggcgcg gcggcgcaaa gaggccgctg cgcgcgcttt ggcgagagcg gaagagcagc
     1861 tgaggcgcgc cgagcgggca gcccgccagc tcgccgcgct tgctgaccgg gtgtctgcca
     1921 ccgccgtggc agtggatgag gcacgcaagg agctctcgga acgcatcgcc gcggagggcc
     1981 ggggcgggag cgaggaggag cggcttcgcc gcgaagtgcg cgaggcacag gcgatcgcgg
     2041 cccaagcccg ggcgcgtctg agagaggccg tggtcgtcgc cgcggcaggc ggcctggtgg
     2101 agcatgtggc cgtgtctgcc ggccaggagt tggccgaagg agaggcgatt gccaccctgc
     2161 gccagctcaa tccggtctgg gcagaagtca ctcttgcccc caatgcggtc aaccgcgtgc
     2221 atcggaagca gaaggtgact ctctcgatcc cctcgtcgga gaggatcgcc gccgtagggg
     2281 agatcgcctc gatggcgccg cgaagcaccc accgggagag cccctggacc gtcaaagtgg
     2341 tcttccccaa tcctcagcac cagcttctcc ccggcgcgga ggttcagctc acgtttcgat
     2401 aggcgggcac ttcccgccac ctcgaaagcg ttggaggcct gtagtggagt cgtttacggg
     2461 ggcagtggca ttcctggtca aaggcgggcc gattatggtc cccctgctgg ccgcctcgtt
     2521 tctcgcggtc gctgtcacga ttgagcgcgc ggtcatactg gcgcgcgcct cgcgcgacag
     2581 cggcccgctg atgcgccgcg tccgggaagc actccagcgg ggggccttcg atgaggcggt
     2641 gcgtgcctgc gaggaggcga acactccggt gggccgggtg ctcgccgccg gactcagggc
     2701 acgccacctc cctccagagc gccttgagaa ggtcctcgaa gaagaggcgc tttccgagct
     2761 acctgccctc aaccgccggc tcgtggtgct cgatacggtg atcaccatcg cgccgctcct
     2821 cggccttctc ggaaccgtca ccgggatgat ccggtcgttc ggcatcatgg cggtctcggg
     2881 gatcgaccgc ccccacggca tcacgggtgg cgtggccgaa gccctgatcg ccaccgcggt
     2941 cgggctcgcg atcgcgatca ccacgctcat cgcctacaac tggttcacgg agaaggtgcg
     3001 cgatatcacc tcgcagatgg agatccgcgc gacgcagctc atcaacctgc tggccgaggc
     3061 gcgcgccgag ccgtggagtg gccggcccgg cacggatggc ccttgctacg cgcgcgatgc
     3121 gcgggtggag gcggccgaat gaggctgccg cgtcacgagc agaagaaggc gcggatcgag
     3181 atcatcccga tgatcgacac catcttcttc ctgctcgtct tcttcatgct cgcctcgctg
     3241 gcgatgacgc ggatgaccgg ggtgaaggtc aacctgccaa aggcgaccag tggagagcgc
     3301 accaccgccg cgaagttcgt ggttaccctc accgccaccg ggcgctacta cgtggagaag
     3361 gaacacgcgg ccgatttcga ggccctccgc gcgatggtcg agcgtcgggt gcgcgagaac
     3421 ccggaggccg tggcggtgat caacgccgat aaatcggtgc gccacggcga ggtgatcgct
     3481 ttgatggacc tggtgaaatc cgccggagtg gcgcggatga cagtggccac cg
//
LOCUS       DAOSCV010000438         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16970_length_3523_cov_3.834775, whole genome shotgun sequence.
ACCESSION   DAOSCV010000438 DAOSCV010000000
VERSION     DAOSCV010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16970_length_3523_cov_3.834775"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1081
                     /locus_tag="PLY56_11140"
     CDS             65..1081
                     /locus_tag="PLY56_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ22079.1"
                     /translation="MRTVEELEERLSRPTPGLAEELSRLDGDIMILGCAGKIGPTLAR
                     MARRALDEAGSKRAVIGVSRFSQPGSRDDLERAGIRTIACDLLDRESVMALPDAANVI
                     FMAGMKFGSTGAEATTWAMNAYMPGIVAERFSQSRIVAFSTGCVYPLVPVLSGGSTEE
                     MAPEPIGEYSQSTLGRERIFEYFSRKNGTPVTLFRLNYAVELRYGVLVDVALKVWSGQ
                     PLDLTMGHANVIWQGDANAVALRCLGIAQSPPAILNVTGPETLSLRAIAHRFGELFDR
                     EPVLTGTEANTALLNNASKMFSLFGYPTVPVDTIVKWVAHWVKSGGATLNKPTHYETR
                     DGKF"
     gene            complement(1137..2234)
                     /locus_tag="PLY56_11145"
     CDS             complement(1137..2234)
                     /locus_tag="PLY56_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ22080.1"
                     /translation="MPKAMVFIDGTWLYSNTPRLSEAYGQPDFRVDFGKLPVVLAEEV
                     SKQLGGLPVDLVRTHLFGSYASNYDARDEEAVQRRRDFFDMLKEEYHYEVETFPINFM
                     GRRLRKADRDPGDPFEPQEKCVDISLATSLLYYAAIPSAYDIAIGLVGDRDFKPAFQQ
                     VRRLGKRVAVASIKGSCAPDYADPRDQARVKDFDIIWLDDILHRLELKYERHLLQCES
                     PFHKGKREVWTTFHPRKGQRFFCDLCRQEARLRQDAQRDLLTTSDGENGLCGGSVDAP
                     VGTPLCGIVTRKITERGFGFIGTPDGASYFFHLTDLQAGLDFYEVDEGIEVEFEVRRH
                     PGPDGRAGAAQCVRRHDSGEPRAVVEVRSEE"
     gene            2666..>3523
                     /gene="hflX"
                     /locus_tag="PLY56_11150"
     CDS             2666..>3523
                     /gene="hflX"
                     /locus_tag="PLY56_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ22081.1"
                     /translation="MTRAPEKIRAGQDWGEAPRRHATQKALLLGIDPGDGSFERSMDE
                     LESLCATAGAQVVGQVVQRRDTPDQATYLGSGKAQEAAEQVQELEADLAIVNAELAPT
                     QQRNLSRVLGVTVIDRTQLILDIFAQRARTHEGKLQVELAQLNYLLPRLAGMWTHLER
                     QAGGIGTRGPGESQLESDRRHISRRIQTLKQALKDVARHRARARASRQQVPFPVIALV
                     GYSNAGKSTLLNTITGAHVYADDRLFATLDPTTRKVRLPVGWEVLMTDTVGFVSNLPH
                     HLVAAFRATL"
BASE COUNT          646 a         1105 c         1168 g          604 t
ORIGIN      
        1 ggtataggga agagtaaagc aaacaatggc atctgagaat agcacgattg gcaccgaggc
       61 ccccatccgg acggtggagg agctggaaga gcgcctctcc cggcccaccc ctggcctggc
      121 ggaggagctc tcgcgtctgg atggcgatat catgattctc ggctgtgccg ggaagattgg
      181 tccgacactc gctcgcatgg cgcgccgggc gctggacgag gcgggctcga agcgcgccgt
      241 gattggcgtg agccggttct cgcagccggg ctcccgggat gacctggagc gcgctggcat
      301 ccgcaccatc gcgtgcgacc tcctggatcg cgagagcgtg atggccctgc cggacgccgc
      361 gaacgtcatc ttcatggcgg ggatgaagtt cggctccacc ggcgccgagg caacgacgtg
      421 ggcgatgaat gcctacatgc ccggcatcgt ggcggagcgt ttctcacagt cgcgcatcgt
      481 cgccttctcc accggctgcg tctacccgct cgtcccggtc ctcagcgggg gctccacgga
      541 ggagatggcg cctgaaccga tcggagagta ctcacaatcg acgctcggtc gcgagcgcat
      601 cttcgagtac ttctcgcgca agaacggcac gccggtcacc ctcttccgcc tcaactacgc
      661 ggtggagctg cgctacgggg tactggtgga cgtggcgctg aaggtgtggt ccgggcagcc
      721 gctggacctg acgatggggc acgcgaacgt gatctggcag ggagatgcca acgccgtggc
      781 gcttcgctgc ctggggatcg ctcagtcgcc cccggcgatc ctgaacgtca ccgggccgga
      841 gacgctctcg ctgcgcgcca tcgcgcaccg attcggcgag ctgtttgacc gcgagccggt
      901 cctcaccgga accgaggcga atacggcgct gttgaacaat gcgtcgaaga tgttcagcct
      961 cttcggctat cccaccgtgc ccgtggatac gattgtgaag tgggtggcgc actgggtgaa
     1021 gtcgggaggc gccacgttga acaagccgac ccactatgaa acccgcgacg gaaagttcta
     1081 agcggggggt gtgatggtag ggaggcgggg ccggtcacgc cccgcctccc cgatagctac
     1141 tcctcggaac gcacctcaac caccgctcga ggctcccccg aatcgtgccg gcgcacgcac
     1201 tgcgccgcgc ccgccctgcc atccgggccg ggatgccggc gcacctcgaa ctccacctca
     1261 atcccctcat ccacctcgta gaaatcgaga ccggcctgca ggtcggtgag gtggaagaag
     1321 tagctggcgc cgtccggcgt gccgataaag ccgaagccgc gctcggtgat ctttcgcgtc
     1381 acgattccgc acagcggcgt gcccacaggg gcgtctacac tgccgccgca cagtccattc
     1441 tcgccatccg aggtggtgag caggtcgcgc tgagcgtcct ggcgcaggcg ggcttcctgc
     1501 cggcagagat cgcagaaaaa ccgctgcccc ttgcgcgggt gaaaggtggt ccacacctcc
     1561 cgctttccct tgtggaaggg cgattcgcat tgaaggaggt ggcgctcgta cttcagctcc
     1621 agccggtgga ggatgtcgtc gagccagatg atatcgaagt ccttgacgcg cgcctggtcg
     1681 cgggggtcag cgtagtcggg cgcgcagctc cctttgatgc tggccacggc gacgcgcttg
     1741 ccaagccggc ggacctgttg gaacgcgggc ttgaaatcac ggtcgcccac gagaccgatt
     1801 gcaatatcgt aggcggaggg gatcgccgcg taatagagca acgacgtggc gagtgagata
     1861 tcgacgcact tctcttgggg ctcgaaggga tctccggggt cccggtcggc tttgcgcagc
     1921 cgccggccca tgaagttgat tgggaacgtc tcgacctcgt agtgatactc ctctttgagc
     1981 atatcgaaga agtcgcgccg gcgttggacg gcctcctcat cacgcgcgtc gtagttggag
     2041 gcgtaactcc cgaaaaggtg ggtgcgcacg agatcgacgg ggagcccgcc gagctgcttg
     2101 ctcacctctt cggcgagcac gacgggaagc ttgccgaaat ccacgcgaaa gtccggttga
     2161 ccgtaggcct cactcaatct cggcgtattc gagtacaacc acgtaccatc tataaagacc
     2221 atcgccttag gcatgaactg ctaacctctc tgcttgctac catttttcga cgggcaacag
     2281 cgctgtcacg gggggcgtcg atgaacacgg gcgacggcag ggaggtgccg ctcgccgatg
     2341 cgaacggcaa cgcgcatccg cggcgcgtgt atcatcacgc ctcagaacgt gacagggtgc
     2401 ccccaggggg agactctcgg tccgagatcc acgatatacc gggccgccgg aggagaagat
     2461 ccccggggac acctcgtatt tgtcggtaaa atcctatgtc cattatatcg atggctccaa
     2521 ggagtgtcaa cgcaagcctc gcgatacggc gaacgcctgc tcttcagccg cacgcggcgc
     2581 actgcaaagg ctatgcaacg cttgacagtt gggcgtgaag tgtggtacaa tagtggtgat
     2641 gactactatc cgatctggag gggatgtgac gagagcacct gagaagatcc gcgcgggaca
     2701 ggactgggga gaagcgccaa ggcgacatgc cacccagaag gcgctgttgc tcggaatcga
     2761 tcctggcgac ggcagcttcg agcgctcgat ggatgagttg gagtcgctct gcgccacggc
     2821 aggggcccag gtcgtggggc aggtggtgca gcggcgtgac acgcccgacc aggccaccta
     2881 tctcggctcc ggcaaggcgc aggaggcggc agagcaggtg caggagttgg aggcggatct
     2941 ggccatcgtg aacgcggaac tggcgccgac gcagcagcgc aacctgagcc gcgtgttagg
     3001 cgtcaccgtc atcgaccgga cccagctcat cctggatatc ttcgcccagc gtgcgcgcac
     3061 gcacgagggg aagcttcagg tggaactggc ccagctcaac tatctcctgc cgcgtctggc
     3121 gggaatgtgg acgcacctcg agcgccaggc cggtggcatt ggcacgcgcg gcccgggcga
     3181 aagccagctg gaatcggacc ggaggcatat ctcgcggcgc atccagacgt tgaagcaggc
     3241 gctgaaggat gtggcgcgcc atcgcgcgcg cgcgcgtgct tcgcgtcagc aggtgccctt
     3301 tcccgtcatt gccctggtag gctatagcaa cgccggaaag tccacgctgc tgaacacgat
     3361 caccggcgcc catgtctacg ccgacgaccg gctcttcgcc accctggatc cgactacgcg
     3421 caaggtgcgc ctcccggtcg ggtgggaagt cctgatgacc gataccgtgg gcttcgtgag
     3481 caacctgcct caccacctgg tggctgcgtt tcgcgccacg ctc
//
LOCUS       DAOSCV010000439         3523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16972_length_3523_cov_3.471742, whole genome shotgun sequence.
ACCESSION   DAOSCV010000439 DAOSCV010000000
VERSION     DAOSCV010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16972_length_3523_cov_3.471742"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PLY56_11155"
     CDS             complement(<1..451)
                     /locus_tag="PLY56_11155"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ22082.1"
                     /translation="MPGRLEATHGVICSMPRDLFGYFGWPSVTRMPDGTLVAAASGLR
                     NEHVCPFGRSVICVSRDEGETWSHPRVVNDTPLDDRDTGIVSLGGKELLLTWFTSDNR
                     TYVKPDSPHYPLWSRGLALVNDENVSVYAGAWVPRSTAPMETPSGSST"
     gene            complement(754..2049)
                     /locus_tag="PLY56_11160"
     CDS             complement(754..2049)
                     /locus_tag="PLY56_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22083.1"
                     /translation="MGEEWRRNGGGASGRGQILRALRSRNYRLFFFGQGVSLIGTWTQ
                     QIAMSWLVYRLTNSPFLLGVVGFCSQAPSALMAPFAGTLADHFSRHRILIGTQALSMV
                     QAAMLAILTLTGVIDVWHIVALAIFLGFVNAVDMPTRQSFVVEMLERKEDLSNAIALN
                     SSLFNGARLLGPTLAGMILPLVGEGACFGINAASYLAVILALLAMQVQPRSLPNPEGR
                     MLDGMREGFAYARRSAPIRTIILLLAVVSLVGMPYTVLMPVFAREILKGDARTLGFLM
                     SASGVGALSGALYLASRRSVLGLDRVIALATATFGMGLILFSASHFLWLSLALLVIVG
                     AGGILQMASCNTIVQTIVDEDKRGRVMGIYIMAFAGTAPVGSLLAGALASRIGVSATL
                     QLGGGICVLAALIFAKQLPALKEAARPIYIRLGILPAKK"
     gene            complement(2532..>3523)
                     /locus_tag="PLY56_11165"
     CDS             complement(2532..>3523)
                     /locus_tag="PLY56_11165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22084.1"
                     /translation="GVPAYGSLKEMLAAHPEIDIVDVCTGGFENGSWHFEPVMEALEA
                     GKHVLVEKPISNNIEEARQMVAKAAEKDLYLGCNLNHYFTEPAERAKQYMDEGKVGEL
                     VYCLHKMGFNGGEATYAPSNASRSKGFPYFHLKAFLAHPFSVMRYFCGDITHVQAFVD
                     RPSFRRAAGDVMLSIASIHVRFANGGVGYLLSQRGDATYGLGGWWSFEVAGTRGTFCI
                     ENCVEKCTFWPAPKPGQAGNAAAMSGGGAGGVEVLDTGIKDFGATFPRRIHAFLEDVT
                     NGVPRDMLRASGRDALATLEYTWAVIESYEQGGILVRPNPLPPIHGDPRVLLD"
BASE COUNT          640 a         1194 c         1135 g          554 t
ORIGIN      
        1 aggtggaact gcccgagggc gtttccatcg gcgccgtgct gcgcggcacc caggcgcctg
       61 cgtaaaccga gacgttctca tcattcacga gcgccaggcc ccggctccag agggggtagt
      121 gcgggctgtc cggcttcacg taggtgcgat tatcactggt aaaccaggtg aggagaagct
      181 ccttgccgcc gaggctgacg attcccgtgt ctcggtcgtc tagtggcgtg tcgttaacca
      241 cacgcgggtg gctccacgtc tcgccctcat cccggctgac gcagatcacc gagcgcccga
      301 aggggcagac atgctcgttg cgcaaccccg aggccgcggc caccagcgtg ccatccggca
      361 tccgcgtgac cgacggccac ccgaaataac cgaagaggtc ccgtggcatg gagcagatga
      421 cgccgtgcgt tgcttcgagt ctcccaggca tggtgtgcga actcctctcg ccgcgggccc
      481 aacaggagag cgaggccgct ggctccgccg tgccctgccg ggcttccttt ggcaagccgg
      541 cgcccggagc cccctggtat cggggcgcgc tccgaccggc tcacctccgc gcgcccgctt
      601 agttcgatgc gcatcctgcc aatgcctgcc ggcgctaccg gtctggtttc atcggcgcgg
      661 gaaagaccgc tcttatccga gaccaccgcg gccaaacggc ctctatcgcg aagcagggcc
      721 tcaccgtaga tggtcccgcc gcgagagcgt cagctacttc ttcgcgggca ggatgccaag
      781 gcggatgtag atgggccggg cggcttcctt cagtgccgga agctgtttgg cgaaaatcag
      841 cgccgccagg acgcagatcc cgcctcccag ctgcagagtc gccgaaacgc caatccggct
      901 tgcgagggcg cccgccagca ggcttcccac gggcgccgtg cccgcgaatg ccatgatgta
      961 gatgcccatc acgcgcccgc gcttatcctc atcgacaatc gtctgcacaa tcgtattgca
     1021 ggaagccatc tggaggatgc cgcccgcgcc tacgatgacc agcagcgcaa gcgagagcca
     1081 gaggaagtgc gatgccgaga acagaatgag ccccatgcca aacgtggccg tcgcgagggc
     1141 gatcacccgg tcgaggccca atacgctccg gcgggaagcc aggtaaagcg ccccggagag
     1201 cgctccgacc ccggacgcgg acatcaggaa gccgagcgtg cgcgcgtcgc ccttcaagat
     1261 ctcgcgggcg aagaccggca tcaggacggt gtagggcatg ccgacgaggc tgaccacagc
     1321 gagcaggagg ataatcgtgc gaattggcgc ggaacgccgg gcgtaggcaa acccctcgcg
     1381 catcccatcg agcatgcggc cctcggggtt ggggagcgag cgcggctgaa cttgcatcgc
     1441 gagcagcgcg aggatcaccg cgagatagct cgcggcgttg atcccgaagc acgcgccctc
     1501 cccaacgagc ggcaagatca tgccggccag cgtcggtccc agcaggcgcg cgccgttgaa
     1561 gagcgacgag ttgagcgcaa tcgcgttgct caggtcctcc ttgcgctcaa gcatctccac
     1621 gacaaacgac tggcgcgtgg gcatatccac cgcgttgaca aagccgagga agatagcgag
     1681 cgccacgatg tgccagacgt caatcacgcc ggtgagggtg aggatggcga gcatcgccgc
     1741 ctgcaccatc gagagcgcct gagttccgat caggatgcgg tgccggctga aatgatcggc
     1801 gagagttccg gcgaagggtg ccatgagggc ggagggcgcc tggctgcaaa acccaaccac
     1861 gcccagaagg aagggtgagt ttgtcagccg gtacaccagc cagctcatgg caatctgctg
     1921 cgtccatgtg ccaatgagcg agacaccctg tccaaaaaag aagagccggt agttccgtga
     1981 tcgaagcgcg cgcaggatct gccctcgccc agacgcgccc cccccgttcc tgcgccattc
     2041 ctctcccatt ccaggtggtt cccgcacgcc cctgcttcgc cctcgtggaa gcaggctcgc
     2101 ccgcgcctgc gatgccgccg ctccatcgca gacttctcaa cagctggttt atggcagaga
     2161 gctccctctg atatgatgcc tacttgcttc cgcccggaat ccctacgagg tgcggaaacc
     2221 acctttccgt ctatgttcgc agcgtggatg gtaccgggtt ctatgcgcga agcccgatgc
     2281 cctgcttcag gaacagatcg atcccgggca cacgtaccgc cctgctcatg cgggccggcc
     2341 gccggagagc ggcttcaccc gccacggcgg acacgcggcg gggcatgaac ctcgggagag
     2401 aagagcacca gagggtggtg cacccgcttc cggcaagccc cccggggatg ccggctgcag
     2461 ccggaccggc tgaaccgccc caggccggtg gatgggagag ccggggcgcc atccccggct
     2521 ccccctgaca ctcagtcgag cagcacccgc ggatcgccgt ggatgggcgg aagcgggttc
     2581 gggcgcacga ggatgccgcc ctgctcgtac gactcaatca ccgcccaggt gtactccagc
     2641 gttgccaggg cgtcgcgccc ggaggcgcgg agcatatcgc gcggcacgcc gttggtcaca
     2701 tcctccagga aagcgtggat gcgccgcggg aaggtcgcgc cgaagtcttt gattccggtg
     2761 tcaaggacct cgacgccgcc ggctccgccc ccggacatgg cggccgcgtt cccggcctgg
     2821 cccggcttgg gcgccggcca gaaggtgcac ttctccacgc agttctcgat gcagaaggtg
     2881 ccgcgagtgc ccgcaacctc gaagctccac cagccgccca ggccgtaggt cgcgtcgccg
     2941 cgctgggaga ggaggtagcc aacgccgcca ttagcaaagc gaacgtgaat gctcgcgatg
     3001 gagagcatca cgtcgccggc ggcgcgccgg aagctgggcc gatccacgaa cgcctgcacg
     3061 tgcgtgatgt cgccgcagaa gtagcgcatc acgctgaagg gatgggccag gaacgccttc
     3121 aggtggaagt agggaaagcc cttgctccgc gaggcgttcg agggggcata cgtcgcctcg
     3181 cctccattga atcccatctt gtggaggcag tagaccagct cgcccacctt cccctcgtcc
     3241 atatactgct tagcccgctc ggcgggttcg gtgaagtagt ggttcaggtt gcagccaagg
     3301 taaaggtcct tctccgcggc cttggcgacc atctgccggg cttcctcgat gttgttggag
     3361 atcggcttct cgacgaggac gtgcttcccc gcctcgagcg cctccatcac cggctcgaaa
     3421 tgccagctcc cgttctcgaa gccgccggta cacacgtcga cgatgtcgat ctccgggtgg
     3481 gccgccagca tctccttcag cgacccatac gccggcacgc cat
//
LOCUS       DAOSCV010000440         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16979_length_3522_cov_2.427170, whole genome shotgun sequence.
ACCESSION   DAOSCV010000440 DAOSCV010000000
VERSION     DAOSCV010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16979_length_3522_cov_2.427170"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLY56_11170"
     CDS             <1..361
                     /locus_tag="PLY56_11170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22085.1"
                     /translation="LEELVNYLNATDVYVTPYVNPDQIVSGTLAYAIGCGRAIVSTPY
                     LYAQSSLSENRGLLAEFRNPGSIAEAITRILGEAPLRTCLEDNCYQWGRKMTWHNVAI
                     EYLDAFHRVMPSRRAAA"
     gene            358..1413
                     /locus_tag="PLY56_11175"
     CDS             358..1413
                     /locus_tag="PLY56_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22086.1"
                     /translation="MKGDKPRYPEPNLSYLRLLSDDTGVIQHALYGVPNRPTGYTTDD
                     NARALLVAIQEYERTGCRQALADAANYLAFLYHALTPDNYFHNLMSYDRRWIDARGSE
                     DSHGRALWAAGYVAGSAAPPEIRRAARQVFEDAVPWARSLRSLRAIAFSMMGVAYYSR
                     QWPESPVIAGLPERLSIQLLAAYQRHRSDEWRWFENALTYSNGMLPHALLRGYELTGN
                     EQCRDVAREALEFLTEILVVDGVLQPIGCHGWYARGGTRAWFDQQPVDVMGMVLLYLT
                     AARIFQDERYQELATLSLDWFFGRNALGQVMVNPETGACYDGLTPEGVNGNQGAESQT
                     AYLLAHLAMVRAGLAPS"
     gene            complement(1417..2031)
                     /gene="lexA"
                     /locus_tag="PLY56_11180"
     CDS             complement(1417..2031)
                     /gene="lexA"
                     /locus_tag="PLY56_11180"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ22087.1"
                     /translation="MKELTERQRQILQTIYDQQRASGYPPTVREIGEAVGLSSSCTVQ
                     KHLNALEKKGYIKRNPTRSRTIEILRFPDAHRPVQRVVDVPLLGTITAGRPILAEEHV
                     EGVFSLPQELVGDGQLFMLRVRGDSMIGRGIHNGDYVVVRQQQTARDGEIVVALLGEE
                     ATVKTFYREPDRVRLQPENPTMAPIYCTDLSILGKVVMAIRTFN"
     gene            2225..>3522
                     /locus_tag="PLY56_11185"
     CDS             2225..>3522
                     /locus_tag="PLY56_11185"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ22088.1"
                     /translation="MNYDLLAEIRVALASGDEGLLCERLSAVLPADLADAIKRLGEED
                     RGAILRVLADDAAGALLQEMGKRDAAAMVRLLPPERARRILGDMAADEAADLLRDLPD
                     EAADALLAGMNAEDAEEVRELLEYPENTAGGRMATDFMPVSPAKRVSEVIEDLRAHPP
                     APEMAYYLYVCDEAGRLAGVVSLRDLIAAAPDTRIAEIMTRDVIRVAVDADQREAAEL
                     VARYDLLALPVVDAQERMLGVITADAVLEVLEETATEEILHVSSGAEEQRDRPLSPLQ
                     VRLRRATTAVAAGLAGAAILAAYRQTFQVSLDYLLFIPLVLVVSEVVTGQVMASIRGA
                     IRHDPSLARLGKAIGRELLSTALVVAAAGALIAWLLGDWQQLQPTGTVLGAAVFLVGI
                     AAGIVGGGLPILSFLLRHDPSAVSGSVTAAVANTLSLATYL"
BASE COUNT          575 a         1133 c         1169 g          645 t
ORIGIN      
        1 actcgaagaa ctggtcaatt atctcaacgc caccgacgtt tacgtgaccc cctacgtcaa
       61 cccagaccaa atcgtcagtg gcacgctggc gtatgccatc ggttgcgggc gggcgatcgt
      121 ctccactccg tacctctatg cccaatcctc cctctccgag aatcggggtc tcctggcaga
      181 gttccgaaat ccggggagca tcgcggaggc gatcacgcgc atcctcgggg aggcgcccct
      241 gcgcacctgt ctcgaggaca actgctacca gtggggccgg aagatgacct ggcacaacgt
      301 tgccatcgaa tacctggatg ccttccatcg cgtgatgccc agccggaggg cagcagcgtg
      361 aagggggaca aaccccgtta cccggagccc aacctgagct acctccggct tctgagcgac
      421 gacaccgggg tgatacagca tgctctgtac ggggtgccga atcggcccac cggctatacc
      481 acggacgaca acgcgcgcgc gctgctggtg gcgattcagg agtatgagcg cacgggatgc
      541 cgccaggcgt tggccgatgc ggccaactac ctcgcatttc tctaccacgc gctcacaccg
      601 gacaactact tccacaacct gatgtcctac gaccgccggt ggatcgacgc gcgtggctcg
      661 gaggattctc acgggcgcgc gctctgggcc gctggctatg tcgccggttc agccgctccg
      721 ccggagatcc ggcgcgccgc caggcaggtc ttcgaagacg ccgtgccctg ggcgcgcagc
      781 ctgcgcagtc tgcgcgccat cgcgttcagc atgatgggag tggcctacta ctcgcggcag
      841 tggccggaat cgccggtgat cgccgggctg ccggagcggc tctccatcca gcttctcgcg
      901 gcctatcaac gccaccgttc cgatgagtgg cgctggttcg agaatgcgct cacctattca
      961 aacgggatgc tgccccatgc gctgctgcgc ggttacgagc tgaccgggaa tgagcagtgt
     1021 cgagacgtgg cgcgcgaggc gctggagttt ctgaccgaga tcctagtggt ggatggggta
     1081 ctccagccga tcggctgtca tgggtggtat gcgcgcggcg gaactcgcgc gtggttcgat
     1141 cagcaaccgg tggatgtgat ggggatggtg ctcctctacc tcactgcggc gcggatcttc
     1201 caggacgaac gctaccagga gctcgccacg ctctcgctgg attggttctt cgggcggaat
     1261 gccctggggc aggtgatggt caatccagag acaggggcgt gctacgacgg gctgacgccc
     1321 gagggcgtca atggcaacca gggcgctgaa tcgcagacag cctaccttct ggcacacctg
     1381 gcgatggtgc gcgccggcct tgccccctcg tgagcgtcag ttgaacgttc ggatcgccat
     1441 caccaccttg ccgaggatcg acaggtcggt gcaatagatc ggcgccatcg tggggttctc
     1501 cggctgcagg cgcacccgat ccggctcacg gtagaaggtc ttgacggtgg cttcctcgcc
     1561 cagaagggca accacgatct cgccatcccg agccgtctgc tgctggcgaa ccacgacata
     1621 atcgccattg tggatgccac ggcctatcat gctatcgccg cgtacgcgga gcatgaacag
     1681 ctggccatct cccaccagct cctggggtag cgagaagacg ccctccacgt gctcttcagc
     1741 aaggatgggc cggccggccg tgattgtgcc cagcagcggg acgtcgacca cgcgctgaac
     1801 ggggcgatgg gcatcgggaa atcgcagaat ctcgatggtg cgggagcgcg ttgggttccg
     1861 cttgatgtag cccttcttct ccagggcgtt caggtgcttc tgcacggtgc agctcgaact
     1921 caagcccacc gcctcaccaa tctcacgcac ggtgggggga tacccgctgg cacgctgctg
     1981 gtcgtatatc gtctgcagaa tctggcgctg gcgttccgtg agctccttca tacagcggtt
     2041 ccttcccgcg agagaggcgc gtcctcgggc gcaccctctc acactcccgt ctattgtagc
     2101 atattcggcg aaattaggca aaccgctgtt cggaacccag gtgtttgccg ctagcggacg
     2161 accgtgtgcc gggcccgcgt tgacaggggg ggatcccgcc ggatataatg acgctggcgg
     2221 caccatgaat tatgaccttc tggctgaaat cagggtggca ctcgcctccg gtgatgaggg
     2281 gctcctctgc gagcgcctga gcgcggtact gccggccgat ctggccgatg cgatcaagcg
     2341 cctgggcgag gaagaccggg gcgccatcct ccgcgtgctg gcggatgatg ccgcgggcgc
     2401 gctcctccag gagatgggga agagggacgc agccgctatg gtgcgcttgc tgcctccgga
     2461 gcgtgccagg cgcattctgg gggacatggc ggccgacgag gccgccgacc ttcttcgaga
     2521 cctgcccgat gaagccgcgg atgcgctgct ggctgggatg aatgccgagg atgcggagga
     2581 agtacgcgaa ctcctcgagt atcccgagaa caccgcgggc gggcgcatgg cgacggactt
     2641 catgccggtt tcccccgcga agcgcgtctc ggaggtgata gaggatctgc gcgctcatcc
     2701 ccccgcgccc gagatggcgt actaccttta cgtctgtgat gaggccggcc ggctggcggg
     2761 ggttgtttcg ctgcgcgatc tgatcgcggc cgcgcccgac acccgcatcg cagagatcat
     2821 gacgcgcgac gtgatccgcg ttgccgtgga tgccgatcag cgcgaggcag cggagttggt
     2881 ggcgcgctac gacctcctgg cgctgccggt cgtggacgcg caggagcgga tgctcggggt
     2941 gatcaccgcc gacgccgttc tggaggtgct cgaagagacg gcgaccgagg agattctcca
     3001 tgtgtcatcg ggcgccgagg agcagcggga tcggcccctg tcacccttgc aggttcgcct
     3061 gcggcgcgcg acgaccgccg tggcagccgg gcttgcgggc gccgccatcc tggcggctta
     3121 ccgtcagaca ttccaggtat ctctcgacta cctcctcttc attccgctgg tgctcgtcgt
     3181 gagcgaggtg gtcaccgggc aggtgatggc ttccattcgc ggcgctatcc ggcacgatcc
     3241 gtccctcgcc cgcctgggaa aggcaatcgg gcgtgagctc ctgagcacgg cgctcgtcgt
     3301 cgccgccgcc ggcgcgctga tcgcgtggct gctgggcgac tggcagcagc tccagccaac
     3361 cggcaccgtt ctcggagcgg ctgtgttcct cgttggaatc gccgcgggga tcgtcggggg
     3421 agggctcccc atcctgtcgt tcctgctacg ccatgatccg tcggcagtct ccggttccgt
     3481 gaccgcggcg gttgccaata cgctgagtct ggcgacttac ct
//
LOCUS       DAOSCV010000441         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_16988_length_3520_cov_3.460029, whole genome shotgun sequence.
ACCESSION   DAOSCV010000441 DAOSCV010000000
VERSION     DAOSCV010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16988_length_3520_cov_3.460029"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLY56_11190"
     CDS             complement(<1..274)
                     /locus_tag="PLY56_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22089.1"
                     /translation="MANSLEGMPARTEVIALPRNAKQLTAAIILSACTLPLPSALGEP
                     ARAPAAPAKAPERLLTFDTCTRVIEGDLIEGEKVGLIRLIGVIAPTK"
     gene            complement(337..1185)
                     /locus_tag="PLY56_11195"
     CDS             complement(337..1185)
                     /locus_tag="PLY56_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22090.1"
                     /translation="MHRSAVVGVALGVLLLVPAGLGQKEAGSAPARPSTPTSNARGAG
                     NAQEKAPRERKIKLEAETVDYDEETSTYIATLATVELPDSDATLRADRIVWNRAERWM
                     RATGKPRMWDPQNEITADVIHADLNVKRANAEGNVCLTARPKEAKTETGKRARSRVKE
                     PVVVRCDTIQYFYKDKKGTATGNLRITQKDPKGDRTATGDRLAYDGSEETISLEGNVH
                     VTTTRGEGFKCKQVIFHVKDGAEGFQVKGLTGGVFYVQEDEEEEAGETGASATSGSAP
                     PPASTE"
     gene            complement(1185..1934)
                     /gene="lptC"
                     /locus_tag="PLY56_11200"
     CDS             complement(1185..1934)
                     /gene="lptC"
                     /locus_tag="PLY56_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOJ22091.1"
                     /translation="MSQNRRLERLGILLAVLMSGAALGLEVQQRLASAPKPSPPANAT
                     APLPPATTPDGELLPLPVPEDGKEILGKGKDLIRIQGVHSGRKQYELTAQRLVLTRDE
                     SKTAVEGIRGTLIGNDGKPVAAFTAERAIMHMQTKDLDVEGQVLVESLDREEKVVFST
                     RNMHWSARDERLTCPNPVEVTQPGVLLRGSSMVADARLRKLSLDGGVYLEADAEKVEK
                     GLGVSDGSSSQSGGRTRGTASPASQRAKGGR"
     gene            complement(1941..2597)
                     /locus_tag="PLY56_11205"
     CDS             complement(1941..2597)
                     /locus_tag="PLY56_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOJ22092.1"
                     /translation="MRIRYRRLVFPAAAALLIAVLASGCGGGGSSSSGDRSGVAVTAT
                     ISGNIVSITAHTDRSDIAKVWAEVTGVASTVSLTGSGQNWSGSVAVTAQANTVVTVKV
                     YAQDSLGNVLGPGVAQVKTGEFGTQPTVTGTVVNRDDNKPVVGATVTLGGQTTATDAS
                     GRFVLTGLLAGTTQEGVITKSGFAEARFTVNVTAGTVDVGRITLTATQDLPPPVPVFP
                     "
     gene            complement(2663..3316)
                     /locus_tag="PLY56_11210"
     CDS             complement(2663..3316)
                     /locus_tag="PLY56_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HOJ22093.1"
                     /translation="MPKHAAKWHLLPTARAGLCAIALLAAALIAGCGGGGSSSEPRVY
                     YGTLIISTAPSGARIFIDDQDTGKTTPQEITREVQQEGTQFRIVARLAGYEDAHATVT
                     VRPDEAVPVNLTLKQTRPPAIPPHTITGKVLLQTGGGTVAATNPTVTATEITTNESFT
                     ASLDTAPERAGTYYIYAPPGQYRVTASMPGYQAQTRDVTIVSGDDRRTGVDFTLPPG"
     gene            complement(3326..>3520)
                     /locus_tag="PLY56_11215"
     CDS             complement(3326..>3520)
                     /locus_tag="PLY56_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOJ22094.1"
                     /translation="SPAGRVVRDVVRGRALSAGTNHILWDGRSNAGVALGSGLYLLQL
                     TATTEEGQSVRAVRPIALVR"
BASE COUNT          532 a         1147 c         1160 g          681 t
ORIGIN      
        1 ctttcgtcgg cgcgatgaca ccaatcaagc ggatcaggcc caccttctct ccctcgataa
       61 ggtctccctc gatgacccgc gtgcacgtgt caaaggtgag gagacgctct ggcgctttgg
      121 cgggggcggc gggagcacgt gccggctctc ccagggcaga tgggagaggc agcgtgcagg
      181 cggacaggat gatggcagca gtaagctgct ttgcgttgcg tggcaaggcg atgacctccg
      241 ttcgagcagg cattccctct agggagtttg ccatgcgccc gctcaactcc tcctgccggt
      301 tgctgccggc aagcgaccta tcgcaggcgc cgcaaattac tcggtagagg caggcggcgg
      361 ggcactgcca gaggtggccg acgcaccggt ctccccggcc tcctcttcct catcttcctg
      421 cacgtagaag acgccgccgg tcagcccttt cacctggaag ccttccgccc catccttcac
      481 gtggaagatg acctgcttgc acttgaagcc ctcaccgcgg gtcgtggtca cgtggacatt
      541 gccttcgagg gagatggtct cctcgctgcc gtcataggct aggcggtcgc cggtcgcggt
      601 ccggtcgccc ttcggatcct tctgggtgat ccgcaggttg ccggtggccg tgcccttttt
      661 gtctttgtag aagtactgga tcgtgtcgca gcgaacgacc acgggctctt tgacgcgaga
      721 acgcgcccgt ttgccggtct ccgtcttggc ctctttgggg cgggccgtca ggcagacgtt
      781 gccctcggca ttggcgcgtt tcacgttgag atcggcatgg atgacatcgg cggtgatctc
      841 gttctgaggg tcccacatgc gcggtttgcc ggtcgcgcgc atccatcgct cggcgcggtt
      901 ccaaacgatg cggtcggcgc gcagagtggc gtcggagtcg ggcagctcga cggtcgccag
      961 agtggcgata taggtggatg tctcctcgtc gtaatccacc gtttcggcct cgagcttgat
     1021 cttgcgctct cggggcgctt tctcctgggc gtttcctgct cctcgcgcgt tggaggtcgg
     1081 cgtggacgga cgcgcggggg cagagccggc ctctttctgg ccaaggccag ccggcacgag
     1141 cagaagaact ccgagggcaa cgccgaccac tgcgctacga tgcactagcg gcctcccttc
     1201 gctctttgtg acgctggaga cgctgttccg cgggtgcgcc cgcccgattg cgaggaactc
     1261 ccgtcggata ctccgaggcc tttctccacc ttttcggcat ccgcctccag gtacacgccg
     1321 ccatccagcg agagctttcg gaggcgcgcg tccgcaacca tgctgctccc acgcaggagc
     1381 acgccgggtt gggtgacctc caccgggttc gggcaggtca atcgctcgtc gcgcgcgctc
     1441 cagtgcatgt tgcgcgtgct aaagaccacc ttctcctctc gatccagcga ctccacgagc
     1501 acctgaccct cgacatccag atccttcgtc tgcatgtgca tgatcgcccg ctcagctgtg
     1561 aacgcggcga cgggcttgcc atcgttaccg atcagcgtgc cgcggatccc ctcgacagcc
     1621 gtcttgctct cgtcgcgcgt gaggaccagg cgctgcgcgg tcagctcata ctgcttgcgg
     1681 ccgctatgca ctccctggat gcggatcaga tccttcccct tgcccagaat ctccttgcca
     1741 tcctcgggca cgggaagcgg cagaagctcg ccatcgggcg tggtcgcggg gggaaggggc
     1801 gctgtggcat tggctggcgg ggagggcttg ggcgctgatg ccaggcgctg ctggacctct
     1861 aggcccaggg ccgcgcctga catcagcact gccaggagaa tgccgagtcg ctcgagacgc
     1921 cgattctggc tcatgattga ttaggggaac acgggaacag gaggcggcaa gtcctgcgtt
     1981 gccgtgagcg taatgcggcc cacatccacc gtgcccgccg tcacgttcac tgtaaaccgg
     2041 gcctcggcaa agccgctctt ggtgatcacg ccctcctgtg tcgttcctgc cagcaacccg
     2101 gtcaggacaa aacgcccgct cgcgtcggta gcggttgtct gaccacctag ggtgaccgtg
     2161 gcaccgacga ccggtttgtt gtcgtctcgg ttcaccacgg tgcccgtcac cgttggctgc
     2221 gtgccgaact cgcccgtctt cacctgcgcg acccccggcc cgagaacgtt gccgagggag
     2281 tcctgggcgt agaccttcac ggttaccacg gtgttggcct gggcggttac cgcaaccgag
     2341 ccggaccaat tctgcccgct gccggtgagc gataccgtgc tggcaacccc agtcacctcg
     2401 gcccaaacct tggcgatatc cgagcggtcg gtgtgcgcag taatgctcac gatgttgccg
     2461 gagatggtgg ccgtcacggc cactccgctc cggtcccccg aggatgagga gccgccgccg
     2521 ccacagcccg acgccaggac ggcgatcaga agcgctgccg ccgcggggaa aacgagcctg
     2581 cgataccgga tgcgcatacc agtgttcctc tcttgctcac attataccat gatggtcgag
     2641 cgctcgcaat caccccatgc ggctagccgg gcgggagcgt gaagtccact ccggtccggc
     2701 ggtcatctcc cgagacgatg gtgacgtcgc gggtttgcgc ctgatagccc ggcatgctcg
     2761 ccgtcacgcg atactgtccg ggtggggcgt agatgtagta ggtgcccgcg cgctccgggg
     2821 cggtatccag agaggcggtg aagctctcgt tcgttgtgat ctccgtcgcg gttaccgtag
     2881 ggtttgtggc tgccaccgtg ccaccgccgg tctggagcag tactttgccg gtgatggtgt
     2941 gcggcgggat cgccggaggc cgggtctgct tcagggtgag gttgaccggc accgcctcat
     3001 cggggcgtac cgtgacggtg gcgtgagcat cctcataccc ggcgagccgc gcgacgatac
     3061 ggaactgagt tccctcttgc tgcacctccc gggtgatctc ctgcggtgtc gtcttgccgg
     3121 tatcctggtc atcaatgaag atccgggcgc cggagggcgc ggtgctgatg atcagggtgc
     3181 cgtaatagac gcggggttcg gaggatgagc ccccgccgcc acacccggcg atcagagccg
     3241 ccgcgaggag ggcgatggca cagagcccgg cccgtgccgt cgggagaagg tgccactttg
     3301 ctgcgtgttt cggcatcagt ctgtcctatc tgaccagagc gatggggcgg acggcgcgaa
     3361 cgctctgccc ctcttcggtt gtcgcagtga gttgcagcag atagaggccg gagcccagcg
     3421 cgacgcccgc gttgctgcgc ccgtcccaca aaatgtggtt ggtgccggcg ctcaaggcgc
     3481 ggccacggac gacgtcgcgc acgacgcggc cggccgggct
//
LOCUS       DAOSCV010000442         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17039_length_3511_cov_3.964988, whole genome shotgun sequence.
ACCESSION   DAOSCV010000442 DAOSCV010000000
VERSION     DAOSCV010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17039_length_3511_cov_3.964988"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..3251
                     /gene="ppdK"
                     /locus_tag="PLY56_11220"
     CDS             558..3251
                     /gene="ppdK"
                     /locus_tag="PLY56_11220"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOJ22095.1"
                     /translation="MAEKRVWLFREGDASMKDLLGGKGANLAEMTNLGLPVPPGFTIT
                     TKTCIEYLSNGEKMPAGLMDEVVEALRDVEQNMGKKLGDPANPLLVSVRSGAKFSMPG
                     MMDTVLNLGLNDATLDGIIKLTGNPRFGYDAYRRFIMMFSDIVLSEEYPALKKNNFEH
                     IFDALKAEVGAKQDTDVDAEGLKKLVGQFKEYFKKTTGRDFPQDPMEQLTMAIEAVFK
                     SWNNKRANDYRNFHKIPHDLGTAVNVQTMVFGNMGDDSGTGVAFTRDVATGEKVLYGE
                     FLMNAQGEDVVAGVRTPVHIDEMKKVNPAVYDQFAEIAKSLEKHYTDVMDIEFTVEKG
                     RLFILQCRVGKRTAAAAVKIAVDLVNEGLIDKDTAISRVEPNQIYQLLLPRFDPKEKD
                     RAKSEGRLLAKGLNASPGAAAGQAIFDADKAEELGKEGKAVILVRSETCPDDVHGMLV
                     AKGILTSRGGATSHAAVVARGLGLPCVAGCEAIRVDEEARFFTVNGTTIKEFDDISID
                     GTTGEVFAGKLATIDPNIAENKELQQLLEWADERRRLEVWANADYPRDAERAIGFGAQ
                     GIGLCRTEHMFMEQERLPIVQEMILAETTEQRKAALDRLLPYQRSDFKGIFEAMKGLP
                     VVIRLIDPPLHEFLPAHDDLLVEVTKLECAGNKPEELAAKQKMLAAVEAQREMNPMLG
                     LRGCRLGLMMPEIIGMQVRAILEAAADVKAAGTPVKVKIMIPLVGHVNELTRTREYLE
                     AEAQKVVAERGPIDYSFGTMIEIPRAALTADEIAKVAQFFSFGTNDLTQTTFGYSRDD
                     AEGKFLLKYVEEKILPENPFQTLDQAGVGQLVEIAVRKGREANPSIELGICGEHGGDP
                     ESIAFCHKVGLNYVSCSPFRVPVARLAAAQANIAYTGPKDK"
BASE COUNT          657 a         1145 c         1130 g          579 t
ORIGIN      
        1 tgcaatcatg cccggatgcc atgggaccca gtgctgtgcg aaccccctcg gggccgaggc
       61 atgccgcgcg cggggctgtc gcggaggggc tcgaaggggc cagcgatgaa gccggtggtt
      121 tccatgcctt gggactggat tccctatgga atcctggacc ttcaatggca cagcgttgac
      181 aaggggggcc ccctgggtta taatacatcg gctgtgtctc tagctcttgc ctgtgtgctt
      241 gacacgcaac ccgggagcag gaggcgagac gactgtcggc atggcgcctg ccacggcggg
      301 ctgtgtggag caacgatccc ctgggcagca gtccggcccc ctctcgttgg gtggcccggg
      361 tggcggccag tgcccctccg gcccggggtg ctcttccccc aagactgccg ctcccggcgc
      421 ggccctaggg ctgtcgtatg gagtggggcg cgagttgtat ggcgggtccg accccgcttt
      481 gcccagccgg gcttgcgatc cggttggggt agtgtaagga actgccgcgt gccatgcggc
      541 gcgaaaagga gatgttgatg gctgagaaac gcgtttggct tttccgcgaa ggcgacgcca
      601 gcatgaagga ccttctcggc ggcaaaggtg ccaacctcgc cgagatgacc aacctgggtc
      661 tccccgttcc ccccggcttc accattacca ccaagacgtg cattgagtac cttagcaatg
      721 gcgagaagat gcccgccggg ctcatggacg aagttgtcga ggcacttcgc gatgttgagc
      781 agaacatggg caagaagctc ggcgatccgg cgaacccgct tctcgtttcg gtccgctccg
      841 gcgccaagtt ctcgatgccc ggtatgatgg acaccgtcct caacctgggc ctcaacgatg
      901 ccaccctgga tggcatcatc aagctcaccg gcaacccccg gtttggctat gacgcctacc
      961 gccgcttcat catgatgttc tcggatatcg tcctctcgga ggagtatccg gcgctcaaga
     1021 agaacaactt cgagcacatc ttcgacgcgc tgaaggcgga agtcggcgcc aagcaggata
     1081 ccgacgttga tgccgaaggc ctgaagaaac tcgttgggca gttcaaggaa tacttcaaga
     1141 agaccaccgg tcgcgacttc ccccaggatc cgatggagca gctgaccatg gccatcgagg
     1201 ccgtgttcaa gtcctggaac aacaagcgcg ccaacgacta ccgcaacttc cataagatcc
     1261 cgcatgacct gggcaccgcc gtcaacgtgc agacgatggt cttcgggaat atgggcgatg
     1321 actccggcac cggcgttgcc ttcacccgcg acgtggccac cggcgagaag gtcctttacg
     1381 gcgagttcct gatgaacgcg cagggcgagg acgtggtcgc cggcgtgcgc accccggtcc
     1441 acatcgacga gatgaagaag gtgaacccgg ccgtttacga ccagttcgcc gagatcgcga
     1501 agagcctcga gaagcactac accgatgtca tggacatcga gttcacggtg gagaagggcc
     1561 ggctcttcat cctccagtgc cgcgtcggca agcgcaccgc cgccgccgcc gtgaagattg
     1621 ccgtggacct ggtcaacgag ggcctcatcg acaaggatac ggcgatctcc cgcgtggagc
     1681 ccaaccagat ctaccagctc ctcctgccgc gcttcgatcc gaaggagaag gaccgcgcca
     1741 agagcgaagg ccgcctgctc gccaagggcc tgaatgcctc gcccggtgcc gctgccggcc
     1801 aggcgatctt tgacgccgac aaggcggaag agctcggcaa ggaaggcaaa gcggtcatcc
     1861 tcgttcgctc cgagacctgt ccggacgacg tgcatggcat gctcgtcgcc aagggtatcc
     1921 tgacgtcgcg cggtggcgcc acctcgcacg ccgctgtcgt tgcccgcggt ctgggcctcc
     1981 cctgtgtggc cggctgcgaa gcgatccgcg tggatgagga agcccgcttc ttcaccgtca
     2041 acggcacgac gatcaaggag ttcgatgaca tctccatcga tggcaccacc ggtgaggtct
     2101 tcgccggcaa gctggcgacc atcgacccga acatcgccga gaacaaggag ctgcagcagc
     2161 tgctggagtg ggctgacgag cgccgccgcc tcgaggtgtg ggcgaacgcc gactacccgc
     2221 gcgacgccga gcgtgctatc ggcttcggcg cccagggcat cggcctctgc cgcaccgagc
     2281 acatgttcat ggagcaggag cgcctcccga tcgttcagga gatgatcctg gccgagacca
     2341 ccgagcagcg caaggccgct ctcgaccggc tcctgccgta tcagcgctcg gacttcaagg
     2401 gcatcttcga ggcgatgaag ggccttcccg tggtcatccg cctcatcgac ccgccgcttc
     2461 atgagttcct gccggcccac gacgatctcc tcgtcgaggt gaccaagctg gagtgcgcgg
     2521 gcaacaagcc cgaggagctg gcagcgaagc agaagatgct ggccgccgtc gaggcgcagc
     2581 gcgagatgaa cccgatgctg ggtctgcgcg gctgccgcct gggcctgatg atgcccgaga
     2641 tcatcggcat gcaggtgcgc gcgatcctcg aggccgcggc cgacgtcaag gctgcgggca
     2701 ccccggtgaa ggtgaagatc atgatcccgc tcgtcgggca cgtgaacgag ctcacccgca
     2761 cccgcgagta cctcgaagcc gaggcgcaga aggtcgtcgc cgagcgcggc ccgatcgatt
     2821 actccttcgg cacgatgatc gagatcccgc gcgcggctct gaccgccgat gagatcgcca
     2881 aggtcgccca gttcttctcc ttcggaacca acgacctgac gcagaccacc ttcggctact
     2941 cgcgtgacga cgccgagggc aagttcctgc tcaagtacgt tgaggagaag atcctgccgg
     3001 agaacccgtt ccagacgctg gaccaggcgg gtgtcggcca gctcgtcgag atcgcggttc
     3061 gcaagggccg cgaggccaac ccgagcatcg agctgggtat ctgcggcgag cacggcggcg
     3121 atccggagag catcgcgttc tgccacaagg ttggcctgaa ctacgtgagc tgctcgccgt
     3181 tccgcgtgcc ggtggcccgc cttgcggcgg cccaggcgaa catcgcctac accggcccga
     3241 aggacaagta agctcgcatc ccgccccttc cctggccccc ggccccgtgc cggaccccag
     3301 ggaagggtgc tccggttcgt tacgaagggg gcggtgcagt tgcaccgccc ccttcgcgcc
     3361 actgtaagac tcccggataa ggggcgtacc ccatgccgcg ggggccggcg cgtttagccc
     3421 cagaagcggt agatctgtag ccggaagagc aggccgataa ggttcaggag ctcgccgatc
     3481 acgaactcgc cgaagatcag ccccaggaag a
//
LOCUS       DAOSCV010000443         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17088_length_3506_cov_3.612286, whole genome shotgun sequence.
ACCESSION   DAOSCV010000443 DAOSCV010000000
VERSION     DAOSCV010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17088_length_3506_cov_3.612286"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /gene="pilM"
                     /locus_tag="PLY56_11225"
     CDS             complement(<1..1308)
                     /gene="pilM"
                     /locus_tag="PLY56_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOJ22096.1"
                     /translation="MGLKAVVGLDIGNTTIKVAEVRVSGGRPRITALGVIPTPPDSVQ
                     QSVVTDPEALTAAIKELLASAGVREKRVVSSVGGQQALVVRIIEVPKMSEKELAETMK
                     WEVERHVPFASSELQMDYKIIDRPDAPPEAQTMDVFLAVAQQEMITTHVDTLIEAGLD
                     PVAIDVEPLALARSLVEVQDPERQKQTVAIVNIGALFTDLSIVRDGTLLFQRTIATGG
                     NSLTEAIAGATNVSREHADRIKKMKAAVDLEAYAVQPAASDPFGGGTEAFGFGMPGFG
                     TPDGGTPAPEEPTLFNPFATAEESAPQELNPFATAEEESAPQELNPFAAAQEESAPQE
                     LNPFAAAEESAPQELNPFAAAEESEPQELNPFATAEEESAPQELNPFATAAEDETIDL
                     GPFAAADGDAQSTPEETASDQDPFGIAALTGGEWESVGDADAGA"
     gene            complement(1871..2560)
                     /locus_tag="PLY56_11230"
     CDS             complement(1871..2560)
                     /locus_tag="PLY56_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22097.1"
                     /translation="MPDLEKLQKLWQIATDQIKKRITNIAVWRAMESAIVLDYEGGTL
                     VLGLPGESYSQSGHLLVPEHRNLIEEVLTQITQRSLTFRVVECGSVEEWKTYKVAEER
                     KRAAQMALINGQRPRAAVDQKSGEQSPVPDAAVDAQSAAIENVNDILNRAYKIFSALP
                     HRTMSQSRARFVRDAAQMLAGAEGQLVASGMDTDTVMRTIDRAIDRIAVWGECDATTV
                     ALEYLRHKERA"
     gene            2808..>3506
                     /locus_tag="PLY56_11235"
     CDS             2808..>3506
                     /locus_tag="PLY56_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ22098.1"
                     /translation="MAITFYNYLTRRKEEFVPLHDPFVGMYVCGPTVYDLPHLGHAKT
                     YVAFDTIVRYLRHRGYKVLYVQNITDVGHLTDELAGEGEDKIIARARLRQVHPMQLAE
                     TYTREYFRAMDALGVLRPDISPRAAGHIPEIIALVQKLIEKGHAYESNGSVYFDVSTF
                     PEYGKLSHREVNAASEEESRVAQEEKRHPADFAIWKRAEPEHILQWPSPWGMGFPGWH
                     IECSAMANKYLGATV"
BASE COUNT          587 a         1138 c         1074 g          707 t
ORIGIN      
        1 cgcgccagcg tctgcatcac caacgctttc ccactcgcct cccgtcaggg cggcgatccc
       61 gaatggatct tggtcgctgg ccgtctcttc gggagtgctc tgagcgtcgc catccgccgc
      121 ggcaaagggc ccgaggtcaa tcgtctcatc ctccgccgcg gtcgcgaagg gattcagctc
      181 ttgcggtgca ctctcttcct ccgccgttgc gaaggggttc agctcttggg gctcgctctc
      241 ctccgctgcc gcgaagggat ttagctcctg tggcgcgctc tcctcggcgg ccgcaaacgg
      301 atttagctcc tgtggtgcgc tctcttcctg cgctgccgcg aaggggttca gctcttgcgg
      361 tgcactctcc tcctccgccg ttgcgaaggg gttcagctct tgtggtgcac tctcctccgc
      421 ggtcgcgaag ggattgaaca gcgtgggctc ctcgggtgcg ggcgtgcccc catctggagt
      481 gccgaagccg ggcatgccaa atccaaaggc ctccgtgcca cccccaaagg gatcggacgc
      541 agcaggctga accgcatagg cctcgaggtc taccgcggcc ttcatcttct taatccggtc
      601 ggcatgctcg cgactgacgt tggtcgcgcc ggcgatcgcc tcggtgagag agtttcctcc
      661 ggttgcgatg gtgcgctgga aaagaagggt gccgtcgcgc acgatgctca gatccgtaaa
      721 gagggcgccg atgttgacga tggccaccgt ctgcttctga cgctccggat cctggacttc
      781 gaccagagaa cgcgccagcg ccagcggctc cacatcgatc gccaccggat cgagccccgc
      841 ctcgatgagg gtgtccacgt gcgtcgtgat catctcctgc tgggcaacgg ccaggaatac
      901 atccatggtc tgtgcctctg ggggcgcgtc cggccgatcg atgatcttgt aatccatctg
      961 gagttcggag gaggcaaagg gcacgtggcg ctccacctcc cacttcatcg tctcggccag
     1021 ctccttctcg ctcatcttcg gcacttcgat gatgcggacg acgagcgcct gctggcctcc
     1081 gacagagctg accactcgtt tctcgcgcac cccggcgctg gcaagcagct ccttgatggc
     1141 agcagtgagt gcctcgggat cggtgacgac gctctgctgg acactatcag ggggggtggg
     1201 gatcacgcca agagcggtga tgcggggccg cccaccactc acgcgcactt ccgccacctt
     1261 gatggtggtg ttgccgatat ctaagccgac gaccgctttc aagcccatag aaggatgtct
     1321 tccgatgtcg gcgcggctgc gagccggcgc ctcccaatca ctggcatgga atagcgcgac
     1381 aagcttgccc gcggtttgtt ggtagccggt attgctcctc agggataccg tgcgactttc
     1441 aatcgatacg gtacttccga gccgttcccc tccctgctgc tgcagcggag acgagttcgc
     1501 gcttgcccct gtaccgccgt atctcccaga gaaagccgat gaacggcctc gcgtccggcg
     1561 tcgtgggaga agcgggcctc tcgcgcgatg ccggcaggcc gaaatcggca ctagattagc
     1621 acgaaagcga gccgcatgtc aagatgcgct tcgaagcctg acaggcgctc cgtgctcact
     1681 aatctcctta ttgaagcccg cacccccaga ttccttcacg ccggccgacc ttatcgccct
     1741 atttttcact ttcaacacgc gcgcctctct cgaaaggctg gcttcgcccc catcgccact
     1801 gggcaacacc cgatagcgcc cggcgattgg gccgccctgg gcgaggggcc cggcggaagg
     1861 agtgcgcgga tcaggcgcgt tccttatggc gcagatactc cagggcgacc gtggtggcat
     1921 cgcactcgcc ccacacggcg atccgatcaa tggcgcggtc aatcgtgcgc atcacggtat
     1981 ccgtgtccat cccggaagcc accagctggc cctccgcgcc tgccagcatc tgggccgcgt
     2041 cacgaacaaa ccgggctcgc gactgcgaca tggtccggtg cgggagcgcc gagaagatct
     2101 tgtaggcccg gttgaggata tcattcacgt tctcgatcgc ggcgctttgc gcatccactg
     2161 ccgcatcggg cacgggcgat tgctcaccgc tcttctggtc cactgcagcc cgcggcctct
     2221 gaccattgat gagggccatc tgggccgctc gcttgcgctc ttctgcaacc ttgtaggttt
     2281 tccattcctc cacgcttccg cactccacca cgcggaaggt gagcgaccgc tgtgtaatct
     2341 gggtgaggac ctcttcaatc agattgcgat gctcgggaac gaggagatgg ccgctttggg
     2401 agtagctctc gccgggcaag cccaagacta gagtaccccc ctcatagtcc agcacgatgg
     2461 ccgactccat cgcgcgccac acggcgatat tagtgatccg cttcttgatc tgatcggtgg
     2521 cgatctgcca gagcttttgt agcttctcca ggtcaggcac ggtgaggtac tccttcctcg
     2581 cggcacgcgc tgctgtgagc gcgtctgtat cattctaccg cttttgtggt accttggcaa
     2641 ggtggtatgc acctgtctcg cccttgcgcc gcgcggagtc agtgtggtat actggacatt
     2701 gatggaagcg gcggcattcg tggcttggag tggtgacttg ccgcatgcag gaaggccggc
     2761 ctggccgttt ccggtggtgc cgctcgccgc aggatgggga ttctctggtg gccataacat
     2821 tctataacta cctcactcga cgcaaagagg agtttgtccc gctgcacgat cccttcgtgg
     2881 ggatgtacgt gtgcgggccg acggtgtacg atctgccgca ccttgggcat gccaagacat
     2941 acgtcgcctt cgacacgatc gttcgctacc tgcgacaccg cggctacaag gtgctgtatg
     3001 ttcagaacat caccgacgtg ggtcacctca cggacgagct ggccggcgaa ggcgaggata
     3061 agatcatcgc tcgcgcgcgc ctgcgccagg tacacccgat gcagctggcg gagacgtaca
     3121 ctcgcgagta cttccgggcg atggacgccc tgggcgtgct ccgaccggat atttcgccgc
     3181 gcgccgcggg gcatatcccg gagatcatcg cgctcgtcca gaagctcatc gagaaaggcc
     3241 acgcctacga gtccaacggc tcggtctact tcgatgtcag caccttcccg gagtatggca
     3301 agctctcgca tcgcgaagtg aacgccgcgt ccgaggagga gagccgggtg gctcaggagg
     3361 agaagcggca tccggcggat ttcgccatct ggaagcgggc cgagcccgag catatcctcc
     3421 agtggccgag tccctgggga atgggcttcc ctggctggca catcgagtgc tctgccatgg
     3481 cgaacaagta cctgggcgcc accgta
//
LOCUS       DAOSCV010000444         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17144_length_3499_cov_3.297909, whole genome shotgun sequence.
ACCESSION   DAOSCV010000444 DAOSCV010000000
VERSION     DAOSCV010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17144_length_3499_cov_3.297909"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLY56_11240"
     CDS             <1..1380
                     /locus_tag="PLY56_11240"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22099.1"
                     /translation="WGGGIYEAPEFYDFCDEFGILVWQDFMFSFAEHPDTEEFAAAVG
                     REAHAVVARLRNHPCLALWCGNNECELDTPPEQQWHGKRLFYEVIPQAVRELDPERPY
                     WPSSPYSGPVAASCEYGDYHGEPWFHASKSGLETWRDWIDKDKGLFMSEFPLHGPPEV
                     ETLRRFIPEEKLFPVEGKLWEAHIITDAHRKQKEGMTSRESLLKNIREMAGEPRSIEE
                     FCYLGGVAHGEFLRAQIEHYRRRKLGVGGALLWMHDEAFPSVSYGIIDHYGRPKPAYY
                     YARRAFAPLLLVFDRDGDEMAAWVVNDLREPVEGVTSLLRIQEGRADAGPERVAFRVP
                     ANAAQRIWTGPPPKDPAREWLAGTLERDGRTVARSSYFLALFREIHFPPASVAVDRSF
                     ADGTLELDLRSDVYAFAVALEGLPDGARPDDNYLDLLPGETRRITVRGLSPEDAAGVR
                     VKTWPRTSG"
     gene            1534..2586
                     /locus_tag="PLY56_11245"
     CDS             1534..2586
                     /locus_tag="PLY56_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ22100.1"
                     /translation="MKAAVKTAKGDFEVREVETPTIPHPNWVRARVRVAGICGTDLRH
                     WRVEDPHLKGKIVGHELAGEIVEVGDEVTDLKEGDRVVVETVLGDDTCEWCRAQQYNL
                     CPHLYEVREKSVSRAFAEYVIGPAKKFYKLPDHVSFEEAALLDTFTVGLHAMHVTNLS
                     LNDRVAILGAGPIGLGQLQLAKAAGAKALITDVLDFPLQVAEKLGTDVTVNTKTGDAY
                     KSVMEFTGGRGVDIAFECAGGDALPTTLAQATSFARIGGKVALVGGFDDGPSTFTLEW
                     QRIQKGEIQIIPSASYALWGIYPEMVMCLDLLAFGKLDAKSLVTHRFPLDQINEAFET
                     AEDKERNNSIFVAVTM"
     gene            2642..2830
                     /locus_tag="PLY56_11250"
     CDS             2642..2830
                     /locus_tag="PLY56_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22101.1"
                     /translation="MTGGCGLVAGERNPHSGAEANIEGAFALMDEIPWRALFRAVRDR
                     QGDACPLPMAAGVMPHEL"
     gene            complement(2848..>3499)
                     /locus_tag="PLY56_11255"
     CDS             complement(2848..>3499)
                     /locus_tag="PLY56_11255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ22102.1"
                     /translation="RGVRVVVYGRHQQELDDTLHDLGEAGGQFHGLIADNAYEDQILN
                     VFAETRRVFGDPGILINNAALPAQSVLDTPYDDILYILRVNVLGYLTCMREAIRIMRS
                     NGGGHIVNVGSMSAKVRETGSDVYVATKAAIEGMSESLRKDLGQEGIRISLVEPGMVG
                     TNLHGEPPDVEGQRRMEAEGQMLEAEDLAQAIYCILTQPPRSNVYEMRIGPVKQAI"
BASE COUNT          593 a         1133 c         1212 g          561 t
ORIGIN      
        1 tggggcggcg gcatctacga ggcgcccgaa ttctatgact tctgcgacga gttcggcatc
       61 ctggtctggc aggacttcat gttctccttc gcggagcacc cggataccga ggagttcgcc
      121 gccgccgtcg gccgcgaggc gcatgccgtg gtggcccgcc tgcgcaacca cccctgcctg
      181 gcgctctggt gcggcaacaa cgagtgcgag ctggacacgc cgccggagca gcagtggcac
      241 ggcaagcgac tcttctacga ggtcatcccc caggcggtcc gcgagctgga ccccgagcgc
      301 ccctactggc cgtccagccc ctactccggg cccgtggcgg cctcctgcga gtacggtgac
      361 taccacgggg agccctggtt ccacgcctcg aagagcggcc tggagacctg gcgcgactgg
      421 atcgacaagg acaagggcct cttcatgagc gagttccccc tgcacggccc gccggaggtg
      481 gagacactgc gccggttcat cccggaggag aagctttttc ccgtggaggg gaagctgtgg
      541 gaagcgcaca tcatcaccga tgcccaccgc aagcagaagg aggggatgac cagccgcgaa
      601 tcgctgctga agaacatccg ggagatggcc ggcgagccgc gcagcatcga ggagttctgc
      661 tacctgggcg gcgtggcgca cggcgagttc ctgcgcgcgc agattgagca ctaccgccgg
      721 cgcaagctcg gcgtcggcgg cgccctcctc tggatgcacg atgaggcgtt cccttccgtg
      781 agctacggca tcatcgacca ctacggccgg cccaagcccg cctactacta cgcccggcgt
      841 gccttcgccc cgctcctcct cgtcttcgac cgcgacggcg acgagatggc ggcctgggtg
      901 gtcaacgacc tgcgagagcc ggtcgagggg gtcacctccc tcctccgcat ccaggagggc
      961 agagccgatg ccggcccgga gcgggtcgcc ttccgcgtgc cggcgaacgc cgcgcagcgc
     1021 atctggaccg gaccgcctcc caaagaccca gcgcgcgagt ggctggcggg cacgctggag
     1081 cgcgacgggc gaaccgtggc gcggagtagc tacttcctgg ccctgttccg ggagatccac
     1141 ttcccaccgg cgtccgtcgc cgtggaccgc tccttcgcgg acggcaccct ggagctggac
     1201 ctccggtcgg acgtctatgc cttcgcggtc gcgctggagg gcctgcccga tggggctcgg
     1261 cccgatgata actacctcga cctgctgccc ggcgagacgc ggcgcatcac cgtgcgcggc
     1321 ctgagcccgg aggatgccgc cggcgtgcgc gtgaagacgt ggccacgcac ttccgggtaa
     1381 agtcggggcc gggagcggtc gtcggccggc gcccacgttt cccggggctt caggcggcgg
     1441 aaagaggggc ttgagggggt gcagccccga aagggccgca cccaggacag caccaggacc
     1501 atgcacatga aggcagagga ggactctcga gcaatgaagg ctgcggtgaa gactgccaag
     1561 ggcgacttcg aggtgaggga agtggagacg ccaacgatac cgcacccgaa ctgggtgcgg
     1621 gccagggtca gggtcgccgg catctgcgga acggacctgc gccattggag ggtcgaggac
     1681 ccgcacctga agggcaagat cgtcgggcac gaactcgcgg gggagatcgt tgaggtcggt
     1741 gatgaagtca cggacctcaa ggagggcgac cgggtcgtcg tcgagacggt tctgggggac
     1801 gacacctgcg agtggtgcag ggctcagcag tacaacctct gcccccacct ctacgaggtg
     1861 agggagaaga gcgtctcacg ggccttcgcg gagtacgtaa tcggcccggc gaagaagttc
     1921 tacaagctgc ccgaccacgt gagcttcgag gaggcggctc tcctggatac cttcacggtc
     1981 ggtctccacg ccatgcacgt caccaacctg agtctgaacg acagggtggc gatcctgggc
     2041 gccgggccca tcgggctggg gcagttgcag ttggccaagg ctgccggagc gaaagcactc
     2101 attaccgatg tgctggattt ccccctgcaa gtagcagaga agctgggcac ggacgtgacg
     2161 gtcaacacga agaccgggga cgcttacaag agcgtaatgg agttcaccgg tggccggggc
     2221 gtcgacatcg ccttcgagtg tgccggcggc gatgccctgc cgaccaccct ggcgcaagct
     2281 acctcgtttg ccaggattgg tggcaaggtg gcgctggttg ggggcttcga cgacgggccc
     2341 tccaccttca ccctggaatg gcagcgcatc cagaaaggcg agatccagat catcccaagc
     2401 gcaagctacg cactctgggg gatctacccc gagatggtga tgtgccttga cttgctggca
     2461 ttcgggaagc tggatgccaa gagcctggtt acgcatcgat tcccgcttga ccagatcaac
     2521 gaggcgttcg agaccgccga agataaggag aggaacaact ccatcttcgt ggctgttacg
     2581 atgtaggacc ggggggtggc gaaagcgtgt gggagggcgc ggtggtgcgc cggcggcggt
     2641 catgacgggc ggttgcgggc tcgttgcggg ggagcggaac ccgcactccg gcgccgaggc
     2701 gaacatcgag ggcgccttcg cgctgatgga tgagatcccc tggcgggcgc tcttccgggc
     2761 ggtaagagac cgccagggtg acgcctgccc cctgccgatg gcggcaggcg tcatgcccca
     2821 tgagctctga tgaggcgagc ggaccactca gatggcctgc ttcaccgggc cgatgcgcat
     2881 ctcgtagacg ttgctgcgcg gcggctgcgt gaggatgcag tagatcgcct gggccaggtc
     2941 ctcggcctcc agcatctgcc cctcggcctc catccgccgc tgcccctcca catcgggcgg
     3001 ctcgccgtgc aggttggtac cgaccatgcc tggctccacg aggctgatgc ggatgccctc
     3061 ctggcccagg tccttgcgca ggctctcgct cattccctcg atggcggcct tcgttgccac
     3121 gtagacgtca ctgcccgtct cgcgcacctt ggcggacatc gaaccgacgt tgacgatgtg
     3181 cccgcccccg ttgctgcgca tgatgcggat ggcctcgcgc atgcaggtga ggtagccgag
     3241 cacgttcacg cgcaggatgt agaggatgtc atcatacggc gtgtccagca cgctctgggc
     3301 cgggagagcc gcgttgttga tcaggatccc cgggtcgccg aagacacgcc gcgtctcggc
     3361 gaacacgttg aggatctggt cctcgtaggc gttgtcggcg atcagcccgt ggaactggcc
     3421 gccggcctct cccaggtcgt ggagcgtatc gtccaattcc tgctggtggc gcccatagac
     3481 gaccacacgg acccccctg
//
LOCUS       DAOSCV010000445         3469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17310_length_3469_cov_2.987405, whole genome shotgun sequence.
ACCESSION   DAOSCV010000445 DAOSCV010000000
VERSION     DAOSCV010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17310_length_3469_cov_2.987405"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLY56_11260"
     CDS             <1..710
                     /locus_tag="PLY56_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22103.1"
                     /translation="YLPETLSAPDGTRISPSRARALGRTDCRPIWRADQPPIQYLTHD
                     LGPLLEVLDDRCVSVTCRSAPWRSQDCPLRSDGQIALFETAKGTLIKILITLNTRRPS
                     EHRYRLFGTEGSAEWFSYEGFCRRFDRDRQESQGWERVPIGAAARGTDASTGHGGTDF
                     QLARHFTRALLEGRPAPIDVYRAIEFCLPGIIANRSAEMGGVPLPIPDLRRVPYSGTR
                     FWEAVSLPEEEPPSEES"
     gene            complement(740..1915)
                     /locus_tag="PLY56_11265"
     CDS             complement(740..1915)
                     /locus_tag="PLY56_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOJ22104.1"
                     /translation="MRSDYYDELIRRNDILAVAQELGLAVRGTKCRCLRPEAHAHGDR
                     SPSLSFNRRRNTFHCWVCPDVGGSVIDLVMLARGVSREEAVAYLAARVGMPPPGPREG
                     GRRVPGAAPRRFPTPAPRPRTVPEGVAGRRDAGVRLIREDPVEYTLVPPDRPPPAPEP
                     QLEPEQIARIYSRFLFWCDRIGENNYLTRRRGISRETADRMGIVWVHDAVSIEKRLRD
                     DYAEEELRASGLFNERGLIFRRHPIVFPFYHQGKACFLQGRRVDGREPKYLSVRRPIC
                     CLYNHDILATLSPSDAVVLSEGVIDTLTWLEYGVPAVGILGVGCFKAEWIAEFKDRRV
                     LICLDNDEPGQRAARDLRQRFVAAGQQAFLVTLPEGVKDINELFLEYPRLDGRAIIQ"
     gene            complement(1925..2587)
                     /gene="rpe"
                     /locus_tag="PLY56_11270"
     CDS             complement(1925..2587)
                     /gene="rpe"
                     /locus_tag="PLY56_11270"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ22105.1"
                     /translation="MVKIAPSMLSSDFARLGEEARAAEAAGADYLHLDIMDGRFVPNI
                     TFGPDVVAAIRRSCSIPLDTHLMIEEPERYVDAFAKAGASILTVHAEASLHLHRLLCQ
                     IREAGCRVGVSLNPATPLEMVRHVLSEIDLLLVMTVEPGFGGQSYIEAMTAKIAEARR
                     MLDEYAPHVELEVDGGINARTAPVVVRAGARVLVAGSAVFTHPNGLDGGMEELRASLG
                     LA"
     gene            complement(2587..>3469)
                     /locus_tag="PLY56_11275"
     CDS             complement(2587..>3469)
                     /locus_tag="PLY56_11275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ22106.1"
                     /translation="ADAGIEEIFIAYPLVGEVKLRRLMALCERAHIRVALDSEEVARG
                     ISRAASAAGHTVGVVVEADTGMRRCGLPIGPELVNLCRLVIDLPGLDFIGPMVYQGHL
                     KGTAEERVRRIPDENARLEHLYETLTAAGITWRQVTGGSTPTLYQTHLLKGITEARPG
                     TYVFNDRNTVGVEACTLEDTALHVLATVVSTAVPGQMIVDAGSKTLTSDRYGAGDGKG
                     HGILLEDPGVVISWMSEEHGMADLSGATRKWRIGDRVTIVPNHVCPLVNLHDELVLHR
                     GGEVVGSWKVAGRGRVR"
BASE COUNT          578 a         1214 c         1121 g          556 t
ORIGIN      
        1 actacctccc ggagacgctc tccgcgccag acggtacccg catttccccc tcacgggcgc
       61 gcgctctggg ccgcaccgac tgccgtccga tctggcgcgc ggatcagccc ccgatccaat
      121 acctcacgca tgatcttggc cccttgctgg aggtcctgga tgaccgttgc gtctccgtca
      181 cctgccggag cgcgccctgg cgctcgcagg attgcccctt gcgctccgac gggcagatcg
      241 ccctcttcga gacggcgaaa ggcacgctga tcaagatcct catcaccttg aacacgcggc
      301 gcccgtcaga gcaccgctac cgcctctttg gcaccgaggg aagcgccgag tggttcagct
      361 acgaaggttt ctgccggcgc ttcgaccgcg accggcagga gtcccaggga tgggagcgcg
      421 tgccgatcgg cgccgcggcg cgcggcaccg atgcgagcac cggccacggc ggcaccgatt
      481 tccaactcgc tcgccacttt actcgcgctc tcctggaggg ccgccccgcg cccatcgatg
      541 tctatcgcgc cattgagttc tgcctgccgg gcatcatcgc caaccgctcg gcggagatgg
      601 gaggcgtgcc cctccccatt ccggacctgc gccgcgtacc ctactctggt acgcgctttt
      661 gggaggccgt ctccttgccc gaggaagagc caccgtccga agagtcctga ttcttgggag
      721 gagcgtccac aaagcgcggc tactggatga tcgctctccc atccaggcgc gggtattcca
      781 ggaagagctc gttgatatcc ttcacccctt cggggagggt gacgaggaac gcctgctgcc
      841 cggccgcgac gaaacgctgg cgcaggtcgc gcgcagcgcg ctggcccggc tcgtcgttgt
      901 cgaggcagat gagcacgcgc cggtctttga attcggcgat ccactccgcc ttgaagcagc
      961 ccacgcccag gattccgacg gccggaacgc catactccag ccaagtgagg gtatcgatca
     1021 cgccctcgct cagcacgacg gcgtcgctgg gcgagagggt tgcgaggata tcgtgattgt
     1081 agaggcaaca gatgggccgg cgcacgctca gatactttgg ctcgcgccca tcgacccgcc
     1141 gaccctggag aaagcacgcc ttcccctgat ggtagaaggg gaaaacgatg ggatggcggc
     1201 ggaagatgag ccccctctcg ttgaaaagcc cgctcgcccg caactcctcc tccgcgtaat
     1261 catcgcgcag gcgcttctcg atgctcacgg cgtcgtggac ccagacgatg cccatgcggt
     1321 cggccgtctc ccgtgagatg ccgcgccgcc gggtgaggta gttgttttcg ccgatccggt
     1381 cgcaccagaa gaggaagcgg gagtagatgc gggcgatttg ctccggctca agctgtggct
     1441 cgggcgcggg aggcggccgg tcgggaggca ccaacgtgta ctcaaccggg tcttcgcgga
     1501 tcagccggac cccggcgtcc cgcctcccgg ccacgccttc cgggacggtt ctcggccgcg
     1561 gagccggcgt ggggaagcgg cgcggggcag cgccaggcac gcggcgcccc ccctccctcg
     1621 gccccggtgg gggcatgccc acgcgcgcgg ccagataggc cacagcctcc tcgcgcgaga
     1681 ctccgcgggc cagcatcacc aggtcgatga cgctgccgcc gacgtcgggg caaacccagc
     1741 agtggaaggt gttgcggcgg cggttgaagg agagcgacgg gctccggtcg ccgtgggcat
     1801 gcgcctcggg ccggagacag cggcatttgg taccgcgcac ggcgaggcct agctcctgcg
     1861 cgacggccag gatgtcgttg cgccggatca gctcgtcgta gtagtcgctc ctcaccgcgt
     1921 acctctacgc caggccgaga gacgcgcgca gctcctccat gccgccgtcc aggccgttag
     1981 ggtgcgtgaa gacggccgag ccggccacca gaacgcgcgc gccggcgcgg accaccaccg
     2041 gagcggtgcg ggcgttgatc ccgccatcga cctccagctc cacatggggc gcatactcat
     2101 ccagcatgcg gcgcgcctcg gcaattttgg cggtcatcgc ctcgatgtag gactgccccc
     2161 cgaagccggg ctccacagtc atcaccagca gcaggtcgat ctccgagagg acgtggcgca
     2221 ccatctccag gggtgtcgcc gggttcagcg agacgcccac gcggcatccc gcctcgcgga
     2281 tctggcagag gagacggtgg agatggagcg atgcctcggc gtggaccgtc aggatgctcg
     2341 ctccagcctt cgcgaaggca tccacgtagc gctcgggctc ctcgatcatc aggtgggtat
     2401 cgagagggat ggagcaagag cgacggatcg cggccaccac atccggcccg aaggtgatgt
     2461 tgggcacgaa gcgcccgtcc atgatatcca gatgaaggta atccgcgccg gccgcctcgg
     2521 cagcgcgtgc ctcttcgcca agacgcgcga aatccgatga cagcatcgat ggggcgatct
     2581 tcaccatcaa cgcactctcc ctcgtcctgc caccttccag gagccaacca cctcgccccc
     2641 gcgatggagc accaactcgt cgtgcaggtt gaccagcggg cagacgtggt tgggcacgat
     2701 cgtcacgcgg tcgccgatgc gccacttgcg ggttgccccc gaaagatcgg ccatgccgtg
     2761 ctcctcgctc atccaggaga tgacgacgcc cgggtcctcc agcagtattc cgtgtccttt
     2821 gccatcgccg gcgccgtagc gatcgctggt gagcgtcttc gagccggcat cgacgatcat
     2881 ctggccgggc acggcggtgc tcaccaccgt ggccagaaca tggagcgcgg tatcctccag
     2941 cgtgcatgcc tccacgccca cggtgttccg gtcattgaag acgtaggttc ctgggcgcgc
     3001 ctcggtaatc cccttcaaca ggtgcgtttg gtagagcgtt ggggtcgagc caccggtcac
     3061 ctggcgccag gtgatccccg ccgcggtgag tgtctcgtag aggtgctcca ggcgggcgtt
     3121 ctcgtccggg atcctccgca cgcgctcctc cgccgtgccc ttcagatggc cctggtagac
     3181 catcggaccg atgaagtcga gacccggcag atcgatcacc aggcggcaga ggttcaccag
     3241 ctcggggccg atgggaagcc cgcaacgccg catcccggta tcggcctcca cgacaacacc
     3301 cacggtgtgc cccgcggcgc tcgcagcacg cgagatcccc cgagcaacct cttcagaatc
     3361 gagggcaacg cgaatatggg cacgctcgca cagcgccatc agccggcgca gcttcacctc
     3421 gcccacgagg ggataggcga taaagatctc ctcaatgccg gcatcggcc
//
LOCUS       DAOSCV010000446         3462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17360_length_3462_cov_3.523628, whole genome shotgun sequence.
ACCESSION   DAOSCV010000446 DAOSCV010000000
VERSION     DAOSCV010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17360_length_3462_cov_3.523628"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLY56_11280"
     CDS             <1..233
                     /locus_tag="PLY56_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ22107.1"
                     /translation="GDFNTPPRGVLYRQITTRFGDAFRIAGWGFGHTFRANMPLLRID
                     YLFLGEGLSSRRCFVPPKQVSDHRAVVADLVL"
     gene            420..1022
                     /locus_tag="PLY56_11285"
     CDS             420..1022
                     /locus_tag="PLY56_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOJ22108.1"
                     /translation="MRLSSAILAIMLAVTAAHAAPQPPPGHGGPAGARGGCPMMGMPR
                     AQAGRGGHGAMAAVMAPVRLLLMHRDMLNLTDDQVSRLTSLHAENQKQMARRLADAYA
                     AHIDLMQALTRPDPDRRAVETAVRALGQAVTEFMLTQSRAYVDALQVLTPAQRQQFQR
                     MASLHASGQCPQCRGMMGGMGGMMGGMAPPMAPPPAEGNP"
     gene            1343..2527
                     /locus_tag="PLY56_11290"
     CDS             1343..2527
                     /locus_tag="PLY56_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ22109.1"
                     /translation="MPYTLLAICLMLFAPLLPLQGDELKESPEADLLPLEPVAPQAKT
                     AVSPFVMPPMLLPVPPAETPEAPSATQPILRTVKAYYVPYADDSFEAVRRYVGAVDII
                     AGQWLAVDGNGNLKEAPDFQRNDEVRQIVHAAGKKVYSCVINRDFDRAVLSSILSTAA
                     RRTAAIDRIVAFVEKKGDDGVDLDFEGVRAGHHHGYHAFVKELAEKLHARGKELSIAL
                     DVTWEGAPSPGLDYDLLAQHADSLMPMTYTFGPGRTPHSPIHYLESAARIAMRSMEPS
                     KFMIGIGVYGRDYDLKTGKRTHPNAARLQAILAQYNPRVIFDEETLTKRLAYKDSSGH
                     SHLVHFDDSETIRAKLTRLAEKYGVCSVGFWRMGQEDASLWDVIDIATGRSRPAQTAS
                     RP"
     gene            complement(2619..>3462)
                     /locus_tag="PLY56_11295"
     CDS             complement(2619..>3462)
                     /locus_tag="PLY56_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22110.1"
                     /translation="NPEIDVVCICTPSGLHGEPTIAAAKAGKHVLCEKPLEVTREMMD
                     AMIAACRGAGVKLGGIFQRRTYPTIQAVRRMIQDGKFGKMVLGDAYLKYYRSPEYYKS
                     AGWRGTWELDGGGALMNQGVHGIDEILWLMGPVKSVFARCDHLVRDIEVEDTAVILVE
                     YESGAYGIIEGTTSVYPGMSTRVEVHGELGTAIIDEAKITTLKFLGEEVDAVASEADD
                     TGVASDPRAIATHGHEVLVQDMVDAILEDREPMIPGEEHRKAVDLILAIYESARTRKE
                     VYLS"
BASE COUNT          612 a         1176 c         1068 g          606 t
ORIGIN      
        1 ccggagattt caacacgccc cctcgcggag tgctgtaccg ccagatcacc acccgctttg
       61 gcgatgcctt ccggatcgcc gggtggggct tcgggcacac tttccgggcg aacatgccgc
      121 tcctccgcat cgattacctc ttcctcggcg agggcctctc atcccggcgt tgcttcgtgc
      181 ctccgaaaca ggtttcggac caccgtgccg tcgtcgctga cctcgtcctg taagcgcgcc
      241 ttgcgataga gagctgcggg agcgcctcga gctccccgag cgcatcgcgc cgtttggaat
      301 gcgcatccgc ggggaaaggg aggcaccgga cccactggtt gacggcgctt cctatcccag
      361 acgcgcggtc atccaacgcg caggggatgc ggatgcgcca gctggaagga aaggggcaaa
      421 tgaggctttc aagcgcgatc ctcgcgatca tgctggccgt caccgcggcg cacgccgctc
      481 cgcagcctcc accgggccat gggggtccgg ctggggcaag gggcgggtgc ccgatgatgg
      541 gaatgccgcg ggcgcaggcc ggccggggcg gacatggggc gatggccgcc gtcatggcac
      601 ccgtgcgcct gctcctgatg caccgcgata tgctgaacct gacggatgac caggtgagcc
      661 ggctgacctc gctgcacgcg gagaatcaga agcagatggc ccggcgcctg gccgacgcct
      721 acgcggcgca catcgacctg atgcaggcgc tcacccgtcc agacccggat cggcgcgccg
      781 tcgagacagc cgtgcgtgcc ttggggcagg cggtcaccga gttcatgctg acccaatcgc
      841 gcgcctacgt cgatgcgctc caggtgctca cgcccgcgca gcggcagcaa ttccagcgga
      901 tggcatcgct tcacgcctcc ggccagtgcc cacagtgccg cggcatgatg ggcggcatgg
      961 gcggcatgat gggcggcatg gccccgccca tggcgccacc cccagcagag ggcaatccgt
     1021 agttctccga cctctcgtag caggaagcgc cgttcacctg gggttgacgg cgcttcctcg
     1081 cctctgataa aataccaaca ttcggtcggc tctttcgcca ggccaggtgc agcagatgcc
     1141 tggccgaacc ctcaccaggt cgggcatgag gctggccgga tgtatccggg ctccaccgga
     1201 aagcgagctc acctttccag gcgccagggg atgcagcgcg ttgtcggagg ctctccctgc
     1261 aggcggatcc gcgctattcg cacggatgat gcagagcaag gagaagcgcc gcgtggattg
     1321 atgtgtgtga aggaggacaa cggtgcccta taccctattg gccatctgct tgatgctttt
     1381 cgctccgctc ctgcccctcc agggcgatga actgaaggag tctcccgaag ccgatctact
     1441 cccccttgag ccggtcgctc cccaggcaaa gacggccgta tccccttttg tcatgccacc
     1501 catgctgctt ccggttccgc cggcagaaac cccagaggcc ccgtccgcca cgcagccgat
     1561 tctgcgcaca gtgaaggcct attacgttcc ttatgctgac gatagcttcg aggcggtgcg
     1621 ccgctatgtg ggcgcggtcg atatcatcgc cggccaatgg ctggcggtcg atggcaacgg
     1681 caacctgaag gaggcgcccg atttccagcg caacgacgaa gtgcgccaga tcgtgcatgc
     1741 cgccggcaag aaagtctact cctgcgtcat caatcgcgac tttgatcgcg ctgtgctctc
     1801 cagcatcctg agcaccgccg cgcggcggac tgccgccatc gaccgcatcg tcgccttcgt
     1861 cgagaagaag ggcgacgacg gggtcgatct cgactttgaa ggcgtgcgtg ccggccatca
     1921 ccacggctac cacgccttcg tcaaggagct cgccgagaag ctgcacgcgc gcggcaagga
     1981 gctgagcatc gccctggatg tcacctggga gggcgcgccc agcccgggcc tcgactatga
     2041 cctgctcgcg caacacgccg atagcctgat gccgatgacc tacaccttcg gacccgggcg
     2101 caccccgcat agtccaatcc actacctaga gagcgcagcg cgcattgcga tgcgcagcat
     2161 ggagccctcc aagttcatga tcggcatcgg cgtctacggg cgtgactacg atctgaagac
     2221 gggcaagcga acccatccca acgcggctag gctgcaggcg atccttgccc agtacaatcc
     2281 acgcgtcatc ttcgatgagg aaacgctgac caagcggctg gcctacaaag acagcagcgg
     2341 gcacagccac ctcgtccact ttgatgattc cgagacgata cgcgcaaagc tgacccggct
     2401 ggccgagaag tatggcgtct gctccgtcgg cttctggcgg atgggccagg aagacgcctc
     2461 gctctgggac gtgatcgata tcgccaccgg gcgcagccgg cccgcacaaa cggcgtcgcg
     2521 cccgtaggaa agccaggcca taaccggcgc ttgattggaa acggcccctg ggataccagg
     2581 ggccgttccg tgatcagggg atgtgtcgta ccctcgctct aggagaggta gacctccttg
     2641 cgcgtccgcg ccgattcgta gattgccagg atcagatcga ccgccttgcg gtgctcctcg
     2701 ccagggatca tcggctcgcg atcctccaag atcgcatcca ccatgtcctg aacgagcacc
     2761 tcgtggccat gggtggcgat agcacgcggg tcggacgcga cgccggtatc gtccgcttca
     2821 ctcgcgactg cgtccacctc ctcgccgagg aacttcagcg tggtgatctt cgcctcgtcg
     2881 atgatggcgg tgccgagctc gccatgcacc tccacgcggg tggacatgcc cggatagacg
     2941 ctcgtcgtgc cctcgatgat gccgtaggca ccgctctcgt actcgaccag gatgacggcc
     3001 gtatcctcca cctcgatgtc gcgaaccagg tgatcgcagc gcgcgaagac gctcttgacc
     3061 gggcccatca gccagaggat ctcgtcgatc ccatgcaccc cttggttcat cagcgcgcca
     3121 ccgccatcca gctcccaggt gccccgccag ccggcgctct tgtagtactc cggcgagcgg
     3181 taatacttca ggtaggcatc gcccagcacc atcttgccga acttgccgtc ctggatcatg
     3241 cggcgaaccg cctggatggt tgggtaggtc ctgcgctgga agataccgcc tagcttcacc
     3301 ccagcgccgc ggcaggccgc gatcatggca tccatcatct cgcgggtcac ttccagcggc
     3361 ttttcgcaaa gaacgtgttt tcctgctttg gcggccgcaa tcgtcggttc cccatggaga
     3421 ccactgggcg tgcagatgca caccacgtca atctccgggt tc
//
LOCUS       DAOSCV010000447         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17400_length_3456_cov_3.337842, whole genome shotgun sequence.
ACCESSION   DAOSCV010000447 DAOSCV010000000
VERSION     DAOSCV010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_3456_cov_3.337842"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1867
                     /locus_tag="PLY56_11300"
     CDS             <1..>1867
                     /locus_tag="PLY56_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22111.1"
                     /translation="FPSIPDPEETRAYFRRSADQECEALARIAKADPEKPLPMMPTDR
                     ERMKLIARKDEEMLRRPQEIWRPDPTPLGFPWASQLPGEPVRVALLTPRWRAYDTMEL
                     ARRLEMDVKHVYFDSANALVDTERYPYRAQTGVGPLGAGVVERLATRACTDPTREVIF
                     VAGLNADAIPARVRDTIVEQVRAGKGLFLTGDARAIAAWPKELVATPDEALTAQLLEG
                     FPWEQFHGLGKGERGRLSDKPPFQAYRFGNGRVIVFTARFGSYSCLVPLNSAIEGLDG
                     ATDRILAWHARAMLLAASRPARAQVALQAPQSKPAAGAPFALPAQVKGEGVADLLVRV
                     QDDHDVVVYQGTQKPADGKAQLSLPALPAGRRYFVDVAARNAQRECLGFGCALVEVTG
                     GPEIREIALNPSVRVHPEAVPMVSLTSGGVLACEATVGSLPAGQAASMRWEVRDAFDR
                     LVARAVSPVGANGKAQAQLRLPPAVTVYHQLDSTLLVGERVLAIRSERFTVPVPYPYD
                     DFTVLLWSYPGGDPVIRKTNRASFDLGTEMMDLCHMRGYTDAGAAREYALAARSGLRL
                     VPYVTRIAGEGGEGNVLKPGLFDTKFLERESASIQIATRQAVPYRPAAYTLGDENY"
     assembly_gap    1868..1877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1878..>3456
                     /locus_tag="PLY56_11305"
     CDS             <1878..>3456
                     /locus_tag="PLY56_11305"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ22112.1"
                     /translation="STVEVCAAPETMAEFRKWLQARYGTIEALNAAWKTRYTGFDAIR
                     APMWFAEAAKQKESFAAWFDHRAFMDEAFALAHERFAEVVRKEDPGAKVGWDGFLGYH
                     WQSGYDFHRLSQNLDLNQVYTTYFPQGELVRSFARPGALTGEWSNIVADKEDGFSAVA
                     WHHLFKGHNSSWWWTLWGCDYTPFNPDTSVAAMGEWFFRSVDEIRAGPGRLLVGAKRD
                     DSRIGVWYSHTDYYAARLAEELVPNAPFAGAGWLDDHRGLLQAIEDLGCQYRYVAAAE
                     VEADPKVLSQFKVLFLPLATCLSDKQVAAIRDWVAAGGTLVADGRAGILTSNGVIRQS
                     RPFDDLFGVRSPAGLAAFTRKPVESSIPMLADETLKVSIMEPDVRLAGGHAEFSSGET
                     PIIVSHTFGKGRTLLVNVPFQTYRAVRSTSHADQALEVLGNAIATAGVQPYARVWTAS
                     GRAQCIEQSLFVDGGLKYLALEQDILEPGQPAQEATLTLPQPAFVYDVREGKPMGQGR
                     TSSWRVKISRGRPLLYAL"
BASE COUNT          531 a         1133 c         1193 g          589 t
ORIGIN      
        1 cttcccgagc attcccgacc cggaggagac gcgcgcttac ttccgccggt cagccgatca
       61 agagtgcgag gcgctcgccc ggatcgcgaa ggcggatccg gagaagcccc tcccgatgat
      121 gccgacggat cgggagcgga tgaagctcat tgcgcgcaag gacgaggaga tgctccgccg
      181 cccgcaggag atctggcgtc cggatccgac gccgctcggc ttcccttggg catcgcagct
      241 gccgggcgag ccggttcgcg tggcactcct gacgccgcgc tggcgtgcct atgatacgat
      301 ggagctggcg cgccggctcg aaatggatgt gaagcacgtc tactttgact cggcgaacgc
      361 tctcgtggac acggagcgct atccctaccg cgcgcagacg ggggttggtc cgctcggcgc
      421 gggcgtcgtg gaacgcctgg cgacgcgcgc gtgcaccgat cctacgcgcg aggtgatctt
      481 cgtggccggg ctcaacgccg atgcaatccc ggcgcgggtt cgcgatacga tcgtcgagca
      541 ggtgcgcgca gggaaggggc tcttcctcac cggagatgcc agagcgatcg cggcgtggcc
      601 caaggagctt gtggctacgc cggacgaggc gctgacggcg cagctgctcg agggattccc
      661 ctgggagcag ttccacggcc tgggtaaggg cgagcgcggc cggctgagcg acaagcctcc
      721 cttccaggca taccgcttcg ggaacgggcg tgtgatcgtc ttcaccgccc gcttcggctc
      781 ctactcctgc ctcgtgcctt tgaacagcgc cattgagggt ctcgacggcg cgaccgaccg
      841 gatcctcgcc tggcacgcac gcgccatgct cctggcggct agccggccgg cccgcgcgca
      901 ggtcgctctt caggcgccgc agtctaagcc cgccgcgggc gcgccgttcg cgcttcccgc
      961 gcaggtgaag ggcgagggcg ttgctgacct gctcgtgcgc gttcaggacg accacgacgt
     1021 ggttgtctac cagggaacgc agaagcctgc tgacggcaag gcgcagctct cgctccccgc
     1081 gctccctgcc ggtcggcgct actttgtgga cgtggccgca cgcaatgctc agcgcgagtg
     1141 cctcggcttc gggtgtgcgc ttgtcgaggt cactggtggc ccggagatcc gtgagatcgc
     1201 gctcaacccc tcggttcggg ttcatccgga agcggtgccg atggtctcgc tgacttccgg
     1261 cggcgtgttg gcgtgcgagg cgacggtggg ctcgctgccc gcgggccagg cggcatcgat
     1321 gcgctgggag gttcgcgatg cgttcgaccg gctggttgcc agggctgtct cgcccgtggg
     1381 tgccaacggg aaggcacagg cgcagctgag gctcccgccg gcggtaacgg tatatcacca
     1441 gctcgatagc acgcttctcg tgggcgaacg cgtgctggcc atccggagcg agcggttcac
     1501 cgtgcccgtg ccctacccgt atgacgactt cacggtcctc ctctggtcct atccgggtgg
     1561 cgacccggtc atccggaaga cgaatcgcgc gagcttcgac ctgggcacgg agatgatgga
     1621 tctctgccac atgcgcggct acaccgacgc cggggcggcg cgcgaatacg cgcttgccgc
     1681 gcgctctgga ctgcgccttg tgccctacgt cacgcggatc gccggggaag ggggcgaggg
     1741 gaatgttctg aagccagggc tgttcgatac gaagttcctg gagcgcgaga gcgcatcgat
     1801 ccagatcgcc acccggcagg cggtgcccta ccggcctgcg gcctacaccc tgggcgatga
     1861 gaactacnnn nnnnnnnggt ccaccgtgga ggtgtgcgcg gcgcccgaga cgatggcgga
     1921 gttccgaaag tggctgcagg cgcgctacgg caccatcgag gcgctgaatg cggcctggaa
     1981 gacacgctac accgggttcg atgcgatccg cgcgccgatg tggttcgccg aggccgccaa
     2041 acaaaaggag agcttcgccg cgtggttcga ccaccgcgcc ttcatggacg aggcgtttgc
     2101 cctggcgcat gaacgcttcg ccgaggtcgt gcgcaaggaa gacccgggcg cgaaggtggg
     2161 ttgggatggt ttcctcggct accactggca gtcgggatac gacttccacc gcctctctca
     2221 gaacctggat ctcaaccagg tgtacacgac gtacttccct cagggcgagc tggtccgctc
     2281 cttcgcgcgg ccaggcgcgc tgacgggtga atggagcaac atcgtcgccg ataaggaaga
     2341 cggcttctcg gcggtcgcct ggcaccacct cttcaaggga cacaactcct catggtggtg
     2401 gaccctgtgg gggtgcgact acactccgtt caacccggat acctctgtcg cagcgatggg
     2461 cgagtggttc ttccgatcgg tggatgagat ccgcgctggg cctggcaggc tgcttgtcgg
     2521 cgcgaagcgc gacgatagcc gcatcggcgt gtggtactcg cacaccgatt actacgcggc
     2581 ccggcttgcc gaagagctag tgcccaacgc tccgttcgcg ggcgctggct ggctggatga
     2641 ccaccgcggc ctcctgcagg cgatcgagga tcttggctgc cagtaccgct atgtcgccgc
     2701 agctgaggtc gaggccgatc cgaaggtgct ctctcagttc aaggtgctct tcctgccgct
     2761 ggcgacctgc ctctcggata agcaggtggc ggcgatccgc gactgggtgg cggcaggagg
     2821 cacgctcgtc gcggatggcc gcgcaggcat cctcaccagc aacggcgtga tccggcagtc
     2881 ccgaccgttc gacgacctct tcggcgtgcg cagcccggcc gggcttgcgg cgttcactcg
     2941 caagccggtg gagagctcga ttccgatgct cgcggacgag acgctgaagg tttcgatcat
     3001 ggaaccggac gtgcgcctcg ccggaggtca tgccgagttc agcagcggcg agacccccat
     3061 catcgtctcc cacaccttcg gcaagggccg gaccctcctg gtgaacgtgc cgttccagac
     3121 ctatcgcgcc gtacgctcga ccagccacgc cgaccaggct ctcgaggtgc ttggcaacgc
     3181 gatcgctacc gcgggcgtgc agccctacgc gcgggtatgg acggcgtctg gccgggcgca
     3241 gtgcatcgag cagtcgctct ttgttgacgg cgggctgaag tacctcgcgc tcgagcagga
     3301 tatcctcgag cctggccagc cagcccagga agcgacgctc acgctgccgc agccggcgtt
     3361 tgtctacgac gtgcgcgagg gcaagcccat ggggcagggg cgaacctcct cctggcgggt
     3421 gaagatctct cgcgggcgtc cgctcctcta tgcgct
//
LOCUS       DAOSCV010000448         3456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17403_length_3456_cov_2.723317, whole genome shotgun sequence.
ACCESSION   DAOSCV010000448 DAOSCV010000000
VERSION     DAOSCV010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17403_length_3456_cov_2.723317"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLY56_11310"
     CDS             <1..751
                     /locus_tag="PLY56_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ22113.1"
                     /translation="DDGRVSRFEAHQREMQAALDREVLCVASCGTGGSLLSKGQACVS
                     ERSWEEAGNAPRQQGLDVWPAVTQECADVHARPGQGSWIGIGQPLFALEGTLVGAVMP
                     AGEPLDMEESYRLLLVEDNADVRELLADGLSLMGYAVETAETAEDALCQLETKLPDVL
                     LSDIGLPGMDGLELMRRVRNLAGADRLIAFATTGLGAEEDIRRAREAGFDGYFTKPID
                     LFELDQSIRLALQQVLPGSDESSGGSLTSYD"
     gene            complement(735..1058)
                     /locus_tag="PLY56_11315"
     CDS             complement(735..1058)
                     /locus_tag="PLY56_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22114.1"
                     /translation="MAYYREELRRHHVLSTAEAHAMPAGAVINVAGLAIRPHRPPTRS
                     GRTVVFFSLEDEHGMLDITTFEDVYQRYGKVIYRRDAPAPSERIIRDNTARGQSPGAL
                     LSRKR"
     gene            complement(1058..1252)
                     /locus_tag="PLY56_11320"
     CDS             complement(1058..1252)
                     /locus_tag="PLY56_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22115.1"
                     /translation="MPIRRNILERMILCGCFDSLTPNRKALMFELDGVSSHSTQLSDY
                     TDFEKFSHEARILGTTPAAT"
     gene            complement(1259..1411)
                     /locus_tag="PLY56_11325"
     CDS             complement(1259..1411)
                     /locus_tag="PLY56_11325"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22116.1"
                     /translation="MNQSEKQFTAEPGPAIRVGLMQVEGMAEAEIEAVVGERQREGPF
                     SSLAEF"
     gene            complement(1705..2130)
                     /locus_tag="PLY56_11330"
     CDS             complement(1705..2130)
                     /locus_tag="PLY56_11330"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ22117.1"
                     /translation="MAVRTSEAEWKGDLRGGEGMMKFGGGAFQGRYSYSSRFEEGPGT
                     NPEELIAAAHAGCFSMAFAAQLSEAGFTPARVQTTAKVHLGKVADAFAITRIDLFTEA
                     EVPNIPEGRFREIAEAAKAGCPVSKALASVPITLDAKLV"
     gene            2335..3144
                     /locus_tag="PLY56_11335"
     CDS             2335..3144
                     /locus_tag="PLY56_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22118.1"
                     /translation="MKIAVQLNLLPGSTVRDKQKWAADHGVEGIEVSAGNYPVDRLDQ
                     ARADFESSPVPICSVCGNPTFDFLDPDPAKRRNSIEQCKAYLRFCGEMGATGQIVPPI
                     FGPPRIPDLSPYADAITLEKNLLVELCKELGDVAAEAGSYLLLEPLNRYEQHLLRRQA
                     DGVEIIERCGHPNVALISDFFHMHIEETNTPDALRAAGKHVRHCHLADNTRLEPGTGD
                     IDYVAGMRALLDIGFQDYMAYECGLSGPDRAASLAKSLDYLRGCIEKARAL"
     gene            3161..>3456
                     /locus_tag="PLY56_11340"
     CDS             3161..>3456
                     /locus_tag="PLY56_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22119.1"
                     /translation="MRLKVALVTANAERVPAWAREELAREGIELVARQCRTTEEAVEL
                     ARDADVLWLFGANRVITPESLSQLLRCAAMMRSGSGTDDLPVEEATRLGIVVVNT"
BASE COUNT          580 a         1099 c         1175 g          602 t
ORIGIN      
        1 ggatgacggg cgggtttcgc gcttcgaggc gcaccagcgc gagatgcagg ccgctctcga
       61 tagggaggtc ctctgtgttg cctcttgtgg cacgggtggt agcctcctat ccaaggggca
      121 ggcttgcgtt tccgagcgat cgtgggaaga ggcaggtaac gctccccgac aacaagggct
      181 tgatgtctgg ccggccgtga cccaagagtg cgctgacgtg catgctcgtc cgggccaggg
      241 aagctggatc gggattggac agcctctctt cgcgctcgag gggacgctgg tcggggcggt
      301 catgccggcg ggcgagccgc tggatatgga ggagagttac cgcctcctcc tggttgaaga
      361 taacgcggat gtgcgcgagt tgctggccga tgggctgagc ctgatgggct acgcggttga
      421 aaccgcggag acggccgagg atgcgctctg tcagcttgag acgaagctgc cggatgtcct
      481 cctctctgac atcggcctgc ccggcatgga tggtttggag ctgatgcgcc gggtgcggaa
      541 tctggccggg gccgaccggc ttatcgcttt cgcgaccacg ggcctggggg ccgaggagga
      601 tatccgccgg gcccgagagg ccggcttcga tggctacttc acgaagccga tcgatctctt
      661 tgaattggac cagagtatcc ggcttgcact ccagcaggtg ctccccggtt cggatgagtc
      721 cagcggcggc tcgctcacct cttacgactg agcagcgcgc cagggctttg cccgcgggcg
      781 gtgttgtcac gaatgatccg ctcggaaggc gcaggcgcat cgcggcggta gattaccttt
      841 ccatagcgct ggtacacgtc ctcaaatgtc gtgatgtcca gcatcccatg ctcatcctcc
      901 aggctgaaga agacgacggt gcgcccactg cgcgtgggag gacgatgggg ccggatggcc
      961 agcccggcca cgttgatcac ggctcccgcc ggcatcgcgt gcgcctccgc cgtagagagg
     1021 acgtgatgcc ggcgcaactc ctctcggtag taggccatca ggtggcagcg ggcgttgtcc
     1081 ccaggatgcg cgcctcgtgc gagaacttct caaagtcggt atagtcgctc agctgagtgg
     1141 agtggctcga aacgccgtcc agctcgaaca tcagcgcttt ccggttcggc gtcaggctgt
     1201 cgaagcagcc gcagaggatc atccgctcca ggatgttgcg ccggataggc acgcgccgct
     1261 agaactccgc gagggaggag aaggggccct cccgctggcg ctcgccaacg accgcttcga
     1321 tctcggcttc agccatcccc tccacctgca tcaacccgac gcggatcgcc gggcccggct
     1381 cggcggtgaa ctgcttctcg ctctggttca catccggcag gatgatgagg ggggtgcgag
     1441 ttaggatccg gcagagatgg gcataccccc gggcgtcgcg agcatggacg cagatgaatg
     1501 gaacgggtgt gtttgtttgg tgtagcgagc cttcagtgtt cgccctgttt ccggcggggc
     1561 agggcaggcg tggcgcaaac acaaacgtct gcgctgctgc ggttctgagg cgcccgcact
     1621 ccccatccgt gcctgccccc ttcatcggcg gtatcgccgc gccagatgtg ggaagccgcg
     1681 ccccccggag aggcgcggca gggactacac caatttcgcg tccagcgtga tcggcaccga
     1741 ggcgagcgcc ttcgagacgg ggcaccccgc cttcgcggcc tcggcgatct cccgaaaacg
     1801 tccctcgggg atgtttggga cctcggcttc ggtgaagagg tcgatgcggg tgatcgcgaa
     1861 ggcatcggcc accttgccga gatgcacctt ggccgtggtc tggacgcgcg ccggggtgaa
     1921 gccggcttcg ctgagctgcg cggcgaacgc catgctgaag cacccggcgt gcgctgccgc
     1981 gatcagctcc tcgggattgg tgccggggcc ctcctcaaag cgcgaagagt aggagtagcg
     2041 cccctgaaac gcgccgcctc caaacttcat catcccctct ccgccgcgaa ggtcgccctt
     2101 ccactctgct tcggatgtgc gtactgccat cgttgcctcc caatagccgg tcgagccatg
     2161 ttgaactgct tgcttccttt agactctacc cacctaacgt ttgtttctca cacccctggt
     2221 gcccaggacg cgcgagagac gacagggagg tggagacggg cgtcgaatgc agtgttggca
     2281 ctgcatgggc gtgccagggg ccggcaatcc cggcgccggg aaggtgattg caagatgaag
     2341 atcgcggtac agctcaacct gctccccggg agcactgtca gagataagca aaagtgggcc
     2401 gctgaccatg gagtcgaggg gatcgaggtc tctgctggaa actatcccgt cgaccggctc
     2461 gaccaggcgc gcgccgactt cgagtcgagt ccggtgccca tctgctcggt gtgcgggaat
     2521 ccgacgttcg acttcctgga tcccgatccg gcaaagcggc gcaacagcat cgagcagtgc
     2581 aaggcgtatc tgcggttctg cggcgagatg ggcgccaccg gccagatcgt gcccccgatc
     2641 ttcgggccgc cgcggattcc cgacctctcg ccctacgccg acgcgatcac gctggagaag
     2701 aacctcctgg tcgagctgtg caaggagctg ggcgacgtcg cggcggaagc cggctcctac
     2761 ctccttctcg agccgctgaa ccgctacgag cagcacctcc tccgccggca ggcggatggt
     2821 gtggagatca tcgagcggtg cggccacccc aacgtggcgc tcatctccga cttcttccac
     2881 atgcacatcg aggagaccaa cacgccggac gccctgcgcg ccgctggcaa gcacgtgcgc
     2941 cactgccacc tggcggacaa cacccgcctc gagccgggaa caggcgatat tgactacgtg
     3001 gccggcatgc gcgccctcct cgacatcggc ttccaagact acatggcgta tgagtgtggc
     3061 ctctccgggc cagaccgcgc ggcctccctg gcgaagagcc tcgactacct gcgggggtgc
     3121 atcgagaagg cgcgcgcgct gtaggatggg agaagcgggt atgcgcttaa aggtggcgtt
     3181 ggtgacggcc aacgcggaac gagtgccggc ctgggcgcgc gaggagctcg cccgggaggg
     3241 gatcgagctc gtggcccggc agtgccgcac caccgaggaa gcggtggagc tggcccgcga
     3301 cgccgacgtg ctctggctct ttggcgccaa ccgcgtgatc acgccggaaa gcctctcgca
     3361 gctgctccga tgcgccgcca tgatgcgctc cgggagcggc accgacgacc tccccgtgga
     3421 agaggcgacg cgcctgggca tcgtggtggt gaacac
//
LOCUS       DAOSCV010000449         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17454_length_3446_cov_3.983781, whole genome shotgun sequence.
ACCESSION   DAOSCV010000449 DAOSCV010000000
VERSION     DAOSCV010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17454_length_3446_cov_3.983781"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3446
                     /locus_tag="PLY56_11345"
     CDS             <1..>3446
                     /locus_tag="PLY56_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOJ22120.1"
                     /translation="PGGLIRGYVAAEDGLGGYLRGASARVWLENAAGRTVSWTSGRLD
                     LKGRLTFGLPAPPADELTVFRLYAEVTDSRLRTARAMDAVAITPHPIQLAAVPEAGAL
                     VPGVENRVYIAARYPDGAPARATVRYALGGKTGKVETSALGVASLALVPEDAQTPIAL
                     TATDTQGRVGVYQATLPVARVSPTALALREKLRVLNGRPDRGQIASPFLVRPDRGLYR
                     SGDTLRVEVLSPEREGTLYIDGEAGGQTVFARSVKMESGRASLQIRLPDGVLGTTRIR
                     AYALGSWGDLPYAERAIFVRPAPTPRPSVQPNQPAYEPGHAAQVTLHGGEPGALGFQL
                     ARAEGERHLSDPALGVPGIFLAPLRPGVPGAMTGAEEEVWQHAGEEERQEAALAALAL
                     VPRLDGWSLHEDTYAHKRARLQEQRAASWAWLRWLVLLLLAGLWLLAARLFRHPERSG
                     GRTSSAESAIASTGRFVIPAIVVALAATLLFRLAGPEPVSQGPDRARSLSAALQEERR
                     REAQALARQMPVLPPIVPASAPVPEIALWELGARPDPRAEERHAITIPQEMGPWQLVA
                     DILSGRDAPARATVPLRVETDLAVEFRPPAVARPGDVISVSVRVRNPYAASQTARLKL
                     MAGPGAVVIGESILSEALPGGAGITRHFRVSLPGPPGRVFLTLAATGRRRTVTLRREI
                     ELLPASPETTVTHAGQVTGSVSRTIDLPKGVRQATLSLQPSPGAIAAARLRSLPEELA
                     LSTLERAAGLDIARMAATSQQAGSGAVQALERRFLALLACRHESGAFASRPGDKPDVV
                     STAYAVAILEAAQDAVPGAQPVARAARRWLQQQRRSNAGWPSPWGKTPADAALVAWAL
                     GLDTPPSRDLRARYRVEGSRWEDPYLLSLTALGIGGEPERSDVNPQASPGPGPSLLRR
                     IADQAVAASAGQKWLSSLRTIQGLDGEASATEATALAVLALAPHAEHRKRAEDGARYL
                     ATTRQPDGGWGNLLNTALALRALAAVAPEKPALGAVEVSLNGKKVARLDANQLSVELH
                     AHLRPGQNTLALSAEGTTGAYYELRVALPDVGATRDRAPALVTEIQPRRAHPGQSVTF
                     RARLAPETGGPLSLEVPLPAGFQVLRADLDELARRGEIGGYHVNAQRVWMQVEPGRRR
                     QAR"
BASE COUNT          487 a         1303 c         1148 g          508 t
ORIGIN      
        1 cccgggggcc tgatccgcgg ctatgtggcc gcggaagacg gtctgggtgg ctacctgcgc
       61 ggcgccagcg cccgcgtctg gctggagaac gctgccgggc gcaccgtctc gtggacctct
      121 ggccgtctgg atctgaaagg ccggctcact ttcggtctcc ccgcacctcc ggcggacgaa
      181 ctgacggtct tccggctcta cgcagaggtg accgattcgc ggcttcgcac ggcccgggcg
      241 atggatgccg tggcgatcac cccgcacccc attcaactgg ccgccgtgcc ggaagcaggc
      301 gccctggtgc ccggagtcga gaaccgcgtc tacattgccg cgcgctaccc ggacggcgcc
      361 cccgcgagag ccaccgtgcg ctatgccctc ggtggaaaga ccggcaaagt ggaaacgagc
      421 gcgctgggcg tcgcctcgct ggctctggtg cctgaagatg cgcagacccc catcgcgctg
      481 acagccacgg acacgcaggg ccgcgtgggc gtctatcagg cgactctgcc ggtggcccgc
      541 gtctctccga cggccttggc gctgcgggag aagctgcgcg tcctcaatgg ccggccggat
      601 cgaggccaga tcgcttcgcc cttcctggtt cgtccagacc gtggcctcta ccgctccggt
      661 gacacgctgc gcgtcgaggt gctatcgccc gagcgggaag gcacccttta catcgacggc
      721 gaagcgggcg gccagactgt cttcgcacgt tcggtcaaga tggagtccgg tcgtgcctcg
      781 ctgcagatcc gcctgccgga tggtgttctc ggcaccacgc gaatccgcgc gtatgccctg
      841 ggctcctggg gcgatctccc ctacgccgag cgtgccatct ttgtccgccc tgcgcccacg
      901 ccccggccgt cggtacaacc gaaccagcct gcctacgagc caggccacgc cgcccaggtg
      961 acactgcacg gcggcgagcc gggcgccctg ggattccagc tcgcgcgcgc tgaaggggag
     1021 cgtcacctct ctgatcctgc cctcggcgtt ccaggaatct tcctcgcccc gctgcgcccg
     1081 ggagtgccgg gagcaatgac gggcgcggag gaagaggtat ggcagcacgc aggcgaagag
     1141 gaacgccagg aagcggcact cgcggcactc gccctcgtcc cccggctgga cggctggagc
     1201 ctgcacgagg acacctacgc gcataagcga gcgcgcctcc aggagcagcg ggccgcatcg
     1261 tgggcctggc tgcgctggct ggtgctcctc cttctcgccg ggctgtggct cctggccgcg
     1321 cgtctctttc gccatccgga gcgatccggc ggccgaacca gcagtgccga aagcgccatc
     1381 gcgtccacgg ggcgcttcgt catccccgcc atcgtggtgg ccctcgccgc cacgctcctc
     1441 ttccggctcg ccggtccgga gccggtttcc cagggcccgg atcgcgcgcg cagcttgagc
     1501 gcggcactgc aggaggagcg tcggcgggaa gcccaggcgc tggcgaggca gatgcccgtc
     1561 ctgcccccca tcgtacccgc gagcgcgcca gttccggaga tcgcgctgtg ggaacttggc
     1621 gcccgcccgg atccgcgcgc tgaggagcgc cacgccatca ccatccccca ggagatgggg
     1681 ccctggcagc tcgtcgcgga catcctctcc ggtcgtgatg ctcccgcgcg agccaccgtg
     1741 cctctgcgcg tcgaaaccga cctggccgtc gagtttcgtc cgcccgccgt ggcccgcccc
     1801 ggcgacgtca tcagcgtgag cgtgcgcgtg cgcaatccgt atgccgcttc gcagacggcg
     1861 cgcctgaagc tgatggcagg accgggagcc gtcgtcatcg gcgagtcgat cctcagcgag
     1921 gcgctcccgg gaggcgctgg catcacccgg cacttccgag tctcgctccc cgggcccccc
     1981 ggacgtgtct tcctcaccct cgcggccacg ggacgccggc gcacggtgac ccttcgccgc
     2041 gagatcgaac tccttcccgc ctcgccggag acgaccgtga cgcatgccgg ccaggtgacc
     2101 ggctcggtct cgcgcaccat cgacctcccc aagggtgtgc gccaggccac tttgagcctg
     2161 caaccctctc caggcgccat tgccgcggca cgcctccgct ctcttccgga agagcttgcc
     2221 ctgagcacgc tcgaacgcgc cgccggcctt gatatcgcgc gcatggccgc cacgtctcag
     2281 caggcgggct ccggcgcggt gcaggcactt gaacgccgct ttctcgcgct tctcgcgtgc
     2341 cggcacgagt cgggggcgtt tgcctcgcgc cccggcgaca agcccgacgt ggtcagcacc
     2401 gcctacgccg tcgccattct ggaagcggcg caggatgccg tgccaggcgc ccaaccggta
     2461 gcccgggcgg cgcgccgctg gctgcagcaa cagcgccggt ctaacgctgg ctggccctct
     2521 ccctggggca agacacccgc cgacgcggcc cttgtcgcat gggccctcgg cctcgatacc
     2581 ccgccctcgc gcgacctgag agcgcgctac cgggtggagg ggagccgctg ggaggatccc
     2641 tatctcctgt cactcaccgc cctgggtatc ggcggcgagc ccgaacgcag cgacgtgaat
     2701 ccacaagcgt ctcctggacc cggtccttcc ctgctccggc ggatcgcgga tcaggccgtc
     2761 gccgcaagcg cgggccagaa gtggctctcg tcactgcgaa cgatccaggg tctggatgga
     2821 gaggccagcg ccacggaggc caccgccctg gcggtgctgg cactggcccc ccacgctgag
     2881 caccggaagc gcgccgaaga cggcgcccgc taccttgcca ccacgcgcca gcccgacggg
     2941 ggctggggaa acctcctcaa caccgccctc gcgctgcgcg cgctcgctgc ggtcgcgccc
     3001 gagaagcctg ccctcggcgc cgtggaggtc tccctcaacg gcaagaaggt agcccgcctt
     3061 gatgccaatc agctttcggt ggagctgcac gcccacctgc gcccaggtca gaacaccctc
     3121 gcactgagcg cggagggaac gaccggcgcc tactacgagt tgcgcgtcgc cctgcccgat
     3181 gtcggcgcaa cacgcgaccg cgcgcccgcg ctggtcacgg agatccagcc ccgacgggcg
     3241 caccccggcc agagcgtgac cttccgcgcg cgcctggcgc ctgaaacagg cggtccgctc
     3301 tccctggagg ttcccctacc agctggcttc caggtcctcc gtgccgatct agatgagctc
     3361 gcacgccgcg gcgaaatcgg aggataccac gtgaacgcgc agcgcgtgtg gatgcaggtg
     3421 gagccaggcc ggcgcaggca ggcgcg
//
LOCUS       DAOSCV010000450         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17511_length_3438_cov_2.947384, whole genome shotgun sequence.
ACCESSION   DAOSCV010000450 DAOSCV010000000
VERSION     DAOSCV010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17511_length_3438_cov_2.947384"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..938
                     /locus_tag="PLY56_11350"
     CDS             192..938
                     /locus_tag="PLY56_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOJ22121.1"
                     /translation="MIQVIRRALDILECVANAPETPKGLGEIAERVGLNAATCARILQ
                     TLVAAGYVEQLGPRKGYRLGPMAYALAAQGPYRKDLVHVAQPVLERLAGETQETVLLA
                     VLHQGRRVTLCEVEGTQIVQVRSDRLQLYDPLRTATGRLLLAYLDDTRRKSCLETSET
                     YATAPVSTREQMEAELVQARRDGRVIRTSEGHVVGIAYPVWEGETVAAALGLHLPEHR
                     FTGEHRDRVLEAMERAAREMSERVRARRPA"
     gene            1013..1924
                     /locus_tag="PLY56_11355"
     CDS             1013..1924
                     /locus_tag="PLY56_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ22122.1"
                     /translation="MNAQELKRYAREKGADLVGIAPIARFESLRPERSPISIFPECRS
                     VIVLGRRILRGALRGVEEGTNFNSTYGMFGFRWLEDNFLSRTTYDVTCHIESHGFEAV
                     PLFGYAPDGMPVGHPVAEGKPAPNVIVDLDFAAQAAGLGEIGLGGFFLTPEYGPRQRF
                     ALILTDCVLEPDLVSEKRLCGDCGACADACPFGAIDVEKKVIAGVPGHQMEVATIDYR
                     VCRSCPNGAMAGPGRGDRPDRGAAACARACVVRLEEAGKLANKFENRFRKRKPWALDL
                     FHRPIGADEAGNPAYLGCAARADAREK"
     gene            1952..3112
                     /locus_tag="PLY56_11360"
     CDS             1952..3112
                     /locus_tag="PLY56_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22123.1"
                     /translation="MALAGLPARREGLDPEEKAVKLTAAMVKEAAFRAGAGDVGIANI
                     ERFANAPRQMHPKNIFPDCRSVITIVQPIPRGTYRGITEGTHWANYTFYSYNKLNTLF
                     RPAVTYETACFIEDHGWEAVPLYPGVPERTGSRPPVAPDRPAPDVNIQVRIAAVACGV
                     GEIGWSKVLLTKKFGPRVRIGTILTDAELEPDPLPEPSLCNRCMRCAKECPGGAIPLP
                     GERPTIKIQIEDKCYEWGDVHMGRCTLTHHGLNWEASPFLKKSLPGLDLHVRESTLSE
                     EAAYKMTYSLGQAAWAPSAEFPENSVIPYYRQILSHTGYFAVCGAKGCIRACMAHLEK
                     RGVIEQSEFPTPVFPERPWKLPPPEEDETGGVAEGKFPELYNRPDPNPGGWE"
BASE COUNT          603 a         1163 c         1146 g          526 t
ORIGIN      
        1 gccgggttgt gcgttgcctc cgaggcttcc gattccttat cgaaatccca tccgtctctt
       61 tcctccgtgg tatcgacacc ggcgcgcgtc catgtgggcc gcgccggaca ataccctctt
      121 ccaccccttg actcccaccc agctggcggt gtataattgc gctatacgga aaccaatttc
      181 acaatgcgaa aatgatacag gtcatccgac gagcacttga tattctggag tgtgtggcga
      241 acgccccgga gacgcccaag gggttgggcg agatcgcaga acgggtgggg ctgaacgccg
      301 cgacgtgcgc gcgcatcctg cagaccctgg tcgccgcagg ctacgtggag cagcttggtc
      361 cgaggaaggg ctaccggctg gggccgatgg cctacgcgct ggcggcccag gggccttatc
      421 ggaaggatct ggtccacgtc gcccagccgg tcctggagcg cctggcgggg gaaactcaag
      481 agaccgtgct cctggccgtg ctccatcagg gccggcgggt gaccctgtgc gaggtagagg
      541 ggacgcagat cgtccaggtg cgctcggacc ggctccagct gtatgacccg ctccgcacgg
      601 cgacggggag gctcctactg gcctatctcg atgacacccg ccgcaagagc tgcctggaga
      661 cgagtgagac ctacgccacc gcgcctgtaa gcacgcgaga gcagatggag gcggagcttg
      721 tgcaggcccg ccgcgatggg cgcgtcatcc ggaccagcga gggccatgtc gtcggaattg
      781 cctatccggt gtgggaggga gagacggttg ccgcggctct ggggcttcac ctgccggagc
      841 accggttcac cggcgagcac cgggaccggg ttctggaggc gatggagagg gcagcccgag
      901 agatgagcga gcgcgtgagg gccaggcgcc cggcctgagc cggaaggcgc ggcaacggcg
      961 cgccgtcacc cgcggcggca cgccacattc caaaaggagg agatcacacc tcatgaacgc
     1021 acaggagttg aaacgctacg cccgcgagaa gggcgcggat ctggtgggca tcgcgcccat
     1081 cgcgcgcttc gagagcctgc gccccgagcg cagcccgatc tcgatcttcc cggaatgccg
     1141 gtcggtcatc gtgctgggcc ggcgcattct gcgcggcgcc ctgcgcggcg tggaggaagg
     1201 caccaacttc aacagcacct acggcatgtt cggcttccgg tggctggagg acaacttcct
     1261 ctcgcgcacc acctacgacg tcacctgcca catcgagtcg cacggcttcg aggccgtgcc
     1321 cctcttcggc tatgcccccg atgggatgcc ggtcggacac ccggtcgccg agggcaagcc
     1381 cgcgccgaat gtcatcgtgg acctcgactt cgccgcgcag gccgccggcc tgggcgagat
     1441 cggcctgggc ggattcttcc tcacgcctga atatggaccg cggcagcgct tcgcgctcat
     1501 cctcaccgac tgcgtcctgg agccggacct ggtgagcgag aagcggctct gcggcgattg
     1561 cggcgcgtgc gcggatgcct gcccgttcgg cgcgattgac gtggagaaga aggtgatcgc
     1621 cggcgtgccg ggccaccaga tggaggtcgc cacgatcgat taccgcgttt gtcgctcctg
     1681 ccccaacggc gcaatggccg gaccggggcg cggtgaccgc ccggaccggg gggccgctgc
     1741 atgcgcgcgc gcctgcgttg tgcgcctgga agaggctggc aagctggcca acaagttcga
     1801 gaaccgcttc cggaagcgta agccgtgggc gctcgacctg ttccaccggc ccatcggcgc
     1861 ggacgaggcg ggcaatccgg cctaccttgg ttgcgccgcg agagccgacg cgcgagagaa
     1921 gtagattccg accgttcagg gatcgggccc gatggcgctg gccggcctgc cagcccggcg
     1981 cgagggcctc gatccggagg agaaagccgt gaagctgacg gcagcaatgg tcaaggaggc
     2041 ggcgtttcgc gcgggcgccg gcgacgtagg catcgccaac atcgagcgct tcgccaacgc
     2101 gccgcgccag atgcacccga agaatatctt ccccgactgc cgctccgtga tcaccatcgt
     2161 ccagccgatc ccgcgcggga cgtaccgcgg gatcaccgag ggcacgcact gggcaaacta
     2221 caccttctat tcctacaaca agctcaacac gctcttccga ccggcggtca cctatgagac
     2281 ggcgtgcttc atcgaggacc acggttggga ggcggtgccg ctctacccgg gggtgcccga
     2341 gcgaaccgga tcgcgcccgc ccgtggcgcc cgaccgcccg gcgcccgacg tgaatatcca
     2401 ggtgcgcatc gccgccgtgg catgcggcgt gggcgagatc ggttggtcca aagtcctcct
     2461 cacgaagaag ttcgggccgc gcgtgcgcat cgggaccatc ctcaccgacg ccgagctaga
     2521 gccagacccg ctcccggagc cttcgctgtg caaccggtgc atgcgctgcg ccaaggagtg
     2581 ccccggcggg gcgatccccc tccccggcga gcgccccacc atcaagatcc agatcgagga
     2641 taagtgctac gagtggggcg acgtgcacat gggccggtgt acgctgacac accacggcct
     2701 caactgggag gcgtcgccct tcctcaaaaa gtcactccca gggctggacc tccatgtgcg
     2761 cgagagcacg ctctccgaag aggccgccta caagatgacc tactcgctgg gccaggcggc
     2821 ctgggcgccc tcggcggaat tcccggaaaa ctccgtcatt ccctactacc gccagattct
     2881 cagccacacc gggtatttcg ccgtgtgcgg cgcgaagggc tgcatccgcg cgtgcatggc
     2941 ccatctggag aagcggggcg tcattgaaca gtctgagttc ccaacgcccg tcttcccgga
     3001 gcggccgtgg aagctgcccc caccggagga agacgagaca ggcggcgtcg ccgaggggaa
     3061 gttcccagag ttgtacaacc gccctgaccc gaaccccggc ggctgggagt aaccgggaat
     3121 ctcttcgccg catccattcg cgtgggccag caggagcacg gagctgctgg cccacacgcg
     3181 atacttcccc ctcccggccc caccatcccg gatggctcag gtggcaggac gccctataca
     3241 cgccggcgca ttccaggcag gcttgggcgc gggtcatggc gaatcttccc tcattgcccg
     3301 acatcgggaa agtctggccg cggggaggac gagacgccgg ccactggctg gagcataccc
     3361 gtccttctcg cgagggtaga gaagatgcgt gaactcgctc ccatcgcact acacgaaaca
     3421 gaaaccgcct tccacctc
//
LOCUS       DAOSCV010000451         3427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17586_length_3427_cov_2.943357, whole genome shotgun sequence.
ACCESSION   DAOSCV010000451 DAOSCV010000000
VERSION     DAOSCV010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17586_length_3427_cov_2.943357"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="PLY56_11365"
     CDS             <1..992
                     /locus_tag="PLY56_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOJ22124.1"
                     /translation="RGLADAVFRTRYLQQGSNIVVIGGGTGLSTMLRGLKEYTSNITA
                     VVTVSDDGGSSGRLQRELGVLPPGDIRNCLVALADAEPLMRDVFQHRFNGEVSGLAGH
                     SLGNLFLVAITQLRQGDFEQAVKDAAEVLRIRGNVLPSTVERVSLRAVMADGAVIQGE
                     SNIEKGKGPVAQVALTPENPKPVADAVEAIRRADLIVLGPGSVFTSVIPNLLIPGISE
                     AIARSKALRVYICNVMTQPGETDGFKASDHVRAVSEHAGRRVFDYVLVNERVPRRDLL
                     AKYEVQGQVRVEPDVEKIRQMGFKCIVGDFISETNLVRHDPEKLARAIARLVE"
     gene            1130..2143
                     /gene="gap"
                     /locus_tag="PLY56_11370"
     CDS             1130..2143
                     /gene="gap"
                     /locus_tag="PLY56_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ22125.1"
                     /translation="MAVRVGINGFGRIGRQVFKAIQERHANELEVVAINDLTDAETLA
                     HLLKYDSNYGRYPGEVRFEEKDIIVDGKKILVHAEKDPAQLPWADLGVQIVLESTGFF
                     TDGNKAKAHIDAGAKKVLISAPAKNVDLTVVLGVNDEKYDPAKHNLISNASCTTNCLA
                     PVAKVLLDSFQLERGFMNTIHSYTNDQRILDLPHKDLRRARAAALNMIPTTTGAARAI
                     SLVIPELEGKMDGIAMRVPTPTVSVVDLTAVLGRSATAEEINEAFRAAANGRMKGILG
                     VAEEELVSMDFKGDPRSSIVDAKSTGVLGGNLVKILSWYDNEWGYSCRLADLAALVAS
                     KGV"
     gene            complement(2228..>3427)
                     /locus_tag="PLY56_11375"
     CDS             complement(2228..>3427)
                     /locus_tag="PLY56_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22126.1"
                     /translation="NAVVDHDRLEAVALEWAREVAERFLEAGCQEVALRVDGDRGGCI
                     PRAGRRLAEETGYSAANVRRFSCETGIVVPGDTPAECYAWLRENAWDHWLEWRCRALQ
                     SHWLALRDSLRLVREDSHLVLKLAVPDRDLARPDAWFEEKRAPRDLIRAHGIDPRLYA
                     DDRSFLLERTLDVAHDRLRACLHRENSAPLATFDLQKEVAELYKGKAPSGVELSFAPW
                     TEHGQHLGSEFFPRRGDHWGVSNMAPWGRWLFRPLTHALRHNNPYRISLSGEVFATAG
                     REHDWRRFARAYRALPAVEPAPFAGQVALVTAGGTPDPKAALPEDLLVRNYGVRIGII
                     NDSPQPRRIELCPRRAPVPGERWVDLATGITLARGNGQPGAFQLELEPYDLRVLAILP
                     DAPARAR"
BASE COUNT          581 a         1111 c         1171 g          564 t
ORIGIN      
        1 cgcgtggatt ggccgacgcc gtctttcgca cgcgctacct ccagcaggga agcaacatcg
       61 tcgtgatcgg ggggggaacc gggctctcca cgatgctgcg cggcctgaag gagtatacca
      121 gcaacatcac cgcggtggtg accgtctcgg atgatggggg tagctctggc cggctgcagc
      181 gcgaactcgg agtgctgccg cccggagata tccgcaactg tctggtggcc ctggccgacg
      241 cggagccgct gatgcgcgac gtcttccagc accgcttcaa cggcgaggtg agcggtctcg
      301 ccggacactc gctggggaat ctctttctgg tcgcgatcac ccagctccgt cagggcgact
      361 ttgagcaggc ggtcaaagac gccgccgagg tgctgcggat ccgcggcaac gtgctgccct
      421 ccaccgtcga gcgcgtgagc ctgcgcgcgg tgatggcgga cggagcggtg attcaaggtg
      481 aaagcaatat cgagaagggg aaaggtcccg tcgcgcaagt ggcgcttacg ccggagaatc
      541 cgaagccggt ggcagatgcc gtggaggcga tacgacgggc ggacctgatt gtgctgggcc
      601 cggggagcgt gttcaccagc gtgatcccca acctgctcat ccctgggatc agcgaggcga
      661 tcgcgcgctc gaaggcgctt cgggtgtaca tttgtaacgt gatgacccag cccggggaga
      721 ccgacggctt caaagcctcg gaccacgtgc gggcggtgag cgagcacgcc ggaaggcgtg
      781 ttttcgatta cgtcttggtc aacgagcgcg tgccccggcg cgacctgctc gcgaaatacg
      841 aggtgcaagg gcaggtgcgc gttgagccgg atgtggaaaa gattcgacaa atgggcttca
      901 agtgcatcgt gggcgacttt atcagcgaga ccaatctggt gcgccacgat cccgagaagc
      961 tcgcgcgggc gattgctcgc cttgtcgaat aggtgatgca tgcccctcgg aggcgtccgg
     1021 gctcgcaggg cgccatcccg cccgatgggg ggtgcggcag atggggagcg agggcatgtt
     1081 gacctcgctc ccggacaacg tgggttatct cacgtgagga ggtatttgaa tggcagttcg
     1141 agttggtatt aacggttttg ggcggattgg ccgccaggtt ttcaaggcga tccaggagcg
     1201 ccacgccaac gagcttgagg tcgtggccat caacgatctt accgacgctg agacgcttgc
     1261 ccatctcctg aagtacgact ccaactacgg ccgctatccg ggagaggtcc gcttcgagga
     1321 gaaggatatc atcgtcgacg gcaagaagat cctcgtccac gcggagaagg atcccgccca
     1381 gctcccgtgg gctgacctgg gcgttcagat cgtcctcgag tcgaccgggt tcttcaccga
     1441 tggcaacaag gccaaggcgc acatcgatgc cggcgccaag aaggtgctca tcagcgcccc
     1501 ggccaagaac gtcgacctca ccgtggtgct cggcgtcaac gacgagaagt atgatcccgc
     1561 caagcacaac ctgatctcca acgcgtcgtg cacgaccaac tgcctggcgc cggtcgccaa
     1621 ggtcctcctc gacagcttcc agctcgagcg gggcttcatg aacaccatcc actcgtacac
     1681 gaacgaccag cgcatcctgg atctgccgca caaggatctg cgccgcgccc gcgccgccgc
     1741 gctcaacatg atcccgacga ccacgggtgc cgcccgtgcg atcagcctgg tcatccccga
     1801 gctcgagggc aagatggacg gcatcgcgat gcgcgttccc acgcccaccg tctccgtggt
     1861 ggacctcacc gcggttctcg gccgctccgc gaccgctgag gagatcaacg aggcgttccg
     1921 cgccgccgcc aacggccgca tgaaggggat cctcggggtt gccgaggaag agctcgtttc
     1981 gatggacttc aagggcgatc cgcgcagctc catcgtggac gcgaagtcga cgggtgtcct
     2041 gggcggcaac ctggtgaaga tcctctcgtg gtacgacaat gagtggggtt actcctgccg
     2101 cctggctgac ctcgccgcgc tcgtcgcgtc gaagggtgtg taacctcttc tattccgcag
     2161 atacacgggg ctgccccacg cgggggcagc cccgcggcgt gtacggggtg ctccggtttt
     2221 gcgcggtcta cctggcgcgg gccggcgcgt ccgggaggat cgccaggacg cgcaggtcat
     2281 agggttccag ctccagttgg aacgcgcccg gctggccatt cccgcgggcg agcgtgatcc
     2341 cggtcgccag gtccacccag cgctcgccgg gcaccggcgc ccggcggggg caaagctcga
     2401 tccggcgagg ctgggggctg tcgttgatga tcccgatgcg cactccatag ttgcggacga
     2461 ggagatcttc cggcagcgcg gctttcgggt ccggcgtgcc tcctgccgtc acgagcgcca
     2521 cctggccagc gaagggtgcc ggctcgacgg caggcagcgc ccggtaagcc cgggcgaagc
     2581 ggcgccagtc gtgctcgcgc ccggctgtcg cgaacacctc gccggagagg ctgatccggt
     2641 agggattgtt atggcgcaag gcgtgggtga gcggccggaa gagccagcgg ccccacggcg
     2701 ccatgttcga cacgccccaa tgatcgcccc ggcgggggaa gaactcgctc cccaggtgct
     2761 ggccgtgctc ggtccacggc gcgaaagaga gctctacccc ggagggcgcc ttccccttgt
     2821 aaagctccgc cacctccttt tggaggtcga aagtcgcgag cggcgcgctg ttttcgcggt
     2881 gcaaacaggc gcgcaggcgg tcgtgtgcca cgtccagggt gcgctccagg aggaagctcc
     2941 ggtcgtccgc gtagagccgc gggtcgatgc cgtgggcgcg gatcagatcg cgcggggcgc
     3001 gcttctcctc aaaccacgca tccgggcggg ccaggtcgcg gtcgggcacg gcgagcttaa
     3061 ggacgaggtg cgaatcctct ctcacaaggc ggaggctgtc gcgcagggcg agccagtgcg
     3121 attgaagcgc ccggcaccgc cattccagcc agtggtccca ggcgttctcg cgcagccacg
     3181 cgtagcactc ggctggagta tcgcccggga cgacaatccc ggtctcgcac gagaagcggc
     3241 gcacgttggc cgcgctgtag ccggtctcct cagccagcct ccgcccggcg cgcgggatac
     3301 acccgcctct gtctccatct acccgcagtg ccacctcctg gcacccggct tcgaggaatc
     3361 gctcggccac ctcgcgcgcc cattcgagcg cgacggcctc gaggcggtcg tggtcgacga
     3421 ccgcgtt
//
LOCUS       DAOSCV010000452         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17611_length_3422_cov_3.947728, whole genome shotgun sequence.
ACCESSION   DAOSCV010000452 DAOSCV010000000
VERSION     DAOSCV010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17611_length_3422_cov_3.947728"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..871
                     /locus_tag="PLY56_11380"
     CDS             134..871
                     /locus_tag="PLY56_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22127.1"
                     /translation="MREHTRGFTLIEVLVVIAVIAILMAILLPVLHRTRENARTATCQ
                     SNLRQLGQAFQMYAGDYDGMWPCPGGLLGDLNYWHQSHGGGLEPYLQNAGMGLKSVWV
                     CPSWVGGWESPFPPRTYAMNTALRNPPDVEPWAAANQVRDGIPLALIRDPAGTILLFE
                     GIPNIAKAYPGYGYVGRCGYWDSVKGYDLYPNIRWNNSWLPHQPWHSGMNTYLFCDGH
                     VKRQVPRKYPWVPSAADNQWFVVKWRS"
     gene            complement(954..>3422)
                     /locus_tag="PLY56_11385"
     CDS             complement(954..>3422)
                     /locus_tag="PLY56_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOJ22128.1"
                     /translation="TAAAVVEVPNAAYCNWRRSLAQRVGRYALVVDDLAFRTDSENIE
                     VQTLWETSGGGWQAKMGAIRIPAPAAAALPPGWQAVRALQARVTSEPAGPEHLVRLND
                     LGIVLLRATEAGQWLEMAFTLQEAFSGEVFADFLGYSDRGKVRCLIDGGPAGDEIDTY
                     ASGVQPMRVSLGQQSLAPGEHRLRVQVTGRHTGAEKCNIGLAGLSLRPKGAPAATGSS
                     PTAYEIRSSDPMAARTASGITTLEWHGAVKEGQHRIFFHLLAPAGDTRCDRIAENAAA
                     LSLGDGVKAVAVAGEYLGTRAELAVVAEDHLFGRVLLSAGGTAPLVTASEPVDLDWDF
                     VSGTLHLVAEKETRLTLALAPGASVTLDGKPAKLDAGEAGRATLLLEAGRHRIEGVKP
                     APGARTALSEELTRALAKGQALRTDLAAGMAPASALPPLKESFTANVGDKVVDLVTVS
                     TEAGPVVYAAAGKTVHVLDAGGREIRRMETDGLIRVLHWWPETRLLIAGCTDEKVIAF
                     DERGQRKWVFVSEMDPAVFRAAKQYWFKSAPGHEGIHGLDSGAFLEEGAVPGMQGKTQ
                     LFVGSACTLEILDEAGKLVKRLPVFWGTGSLFRILPGPDGSRNLAVGRSITDGAHLRI
                     VNSKTLSVVGSGFHTVPPGVTYVGGWASMNRWHLFHEDLDGDGTREVVSEINGTWNRV
                     TVWKEDGTALYDASFGPGASIPAKNMRDLDVADLDGDGKKEILAAISGGLVVALDHQC
                     RKVWSRRLPSPPAVMVAVGREPRIVVGCDDGSVVILDGKGTPVQSGRVTGTPVKIGLL
                     QTPAGPAAVLATAKGSVTAFPIQP"
BASE COUNT          565 a         1198 c         1079 g          580 t
ORIGIN      
        1 gcgccgggtt gcgtgctgcc tccgtggctt cggattcctt atctcggaat cccagcccac
       61 gcgttttgcc gcttgaatct ggatagtgga gcatgctata ctacatcagg ttcccccggt
      121 ggaggtcaca tccatgcgtg aacacaccag aggctttacg ctcatcgagg ttctggttgt
      181 aatcgcggtc atcgcgatcc tgatggcgat cctcctaccc gtcctgcacc gcacccgcga
      241 gaatgcccgc acagccacct gccaatccaa cctgcgccag cttggccagg cgtttcaaat
      301 gtacgcgggc gactatgatg gcatgtggcc atgtcctggg gggctgctgg gcgatctgaa
      361 ctactggcac cagtcgcacg gtggcggcct ggagccctac ctgcagaacg caggcatggg
      421 cctgaagagc gtctgggtct gtccctcgtg ggttgggggc tgggaatctc cgtttccgcc
      481 acgcacctat gccatgaaca ctgcgctccg caatccacct gatgtggagc cgtgggcagc
      541 agccaaccag gtgcgcgatg gtattcccct cgccctcatt cgcgatcctg cgggcacaat
      601 tctgctcttc gaggggatcc caaacatcgc gaaggcttat ccgggctatg gctatgtggg
      661 ccgctgcggc tactgggatt cggtgaaggg atatgacctc tatcccaata tccgctggaa
      721 caacagctgg ctaccgcatc agccctggca tagcggcatg aacacctacc tcttctgcga
      781 tggccacgtg aagcgccagg taccccgcaa atacccctgg gttcctagcg ctgccgacaa
      841 ccagtggttt gtggtgaagt ggcgctcgtg aggggacgcg ccgccgtgta cggggagagg
      901 ccggagtggg aggctcctcc cggccgccgg ccctctcccc gccaggcatg cgactatggt
      961 tggattggaa atgccgtcac gctgcccttc gcggtcgcca gcacggcggc cggacccgca
     1021 ggcgtttgca ggaggccaat cttcacgggc gtaccagtga cccggccaga ttgaaccggc
     1081 gtgcccttgc cgtccaggat gacgaccgag ccgtcatcac acccgacaac gatgcgcggc
     1141 tcccgaccaa ccgcgaccat cacagccggc ggactcggca gccggcgcga ccacaccttg
     1201 cggcactggt ggtcgagagc gaccaccagg ccaccggata tggcagccag gatctctttc
     1261 ttgccgtcgc catccaggtc ggccacatcc agatcgcgca tgttcttcgc ggggatcgaa
     1321 gcgcccggcc cgaaactggc gtcgtagagc gcggtgccgt cctctttcca gacggtcacc
     1381 cggttccagg tgccgttgat ctcgctcacc acctcgcgcg tgccgtcgcc atccaggtct
     1441 tcatggaaga ggtgccagcg gttcatgctg gcccacccgc ccacgtaggt gacgccaggc
     1501 ggcacggtgt ggaagccact cccgaccacg gagagcgtct tactattgac gattcgcagg
     1561 tgcgcgccgt cggtgatgct gcggcccacc gccagattgc ggctgccatc gggcccgggg
     1621 agaatccgga agagtgagcc agtgccccag aagaccggca gccgcttcac aagcttgccc
     1681 gcttcatcga ggatctccag ggtgcaggcg ctccccacga agagctgcgt cttcccctgc
     1741 atccccggca cggcgccctc ttcgaggaac gccccgctgt cgaggccgtg gatcccctcg
     1801 tgcccggggg cagacttgaa ccagtactgc ttcgcggcgc gaaagaccgc cggatccatc
     1861 tccgagacga agacccactt acgctggccc cgctcgtcga aggcaatcac cttctcatcg
     1921 gtgcagccgg cgatcagcag gcgtgtttct ggccaccagt ggagcacccg gatcaggccg
     1981 tccgtctcca tgcggcggat ctcccgaccg ccggcgtcga ggacatggac cgtctttccc
     2041 gcagccgcgt agaccaccgg gccggcctcg gttgaaacgg tcaccaggtc caccaccttg
     2101 tcgcccacat tcgccgtgaa actctccttc aaggggggca acgctgacgc gggagccatg
     2161 cccgccgcca ggtccgtccg aagcgcctgg ccctttgcca gagcccgcgt gagctcctcg
     2221 gagagggcgg ttcgtgcccc aggagcaggc ttcacgccct cgatgcgatg gcgaccggcc
     2281 tccaggagga gcgtcgcccg cccggcctcg cctgcatcca gctttgctgg ctttccatcc
     2341 agggtcacgc tcgcgcccgg cgcgagcgcc agagtaagcc gggtctcctt ttcggcaacc
     2401 agatggagcg tacccgatac gaagtcccag tccagatcga cgggctcgct cgccgtgacc
     2461 aggggagcgg ttcccccggc gctgaggagc acacgcccga agaggtggtc ctcggccacc
     2521 acggcaagct ccgcgcgggt gcccaggtac tcgccggcga cggccaccgc cttcacgcca
     2581 tcgcccagtg agagcgccgc agcgttttcg gcgatccggt cgcagcgcgt gtcgccagcg
     2641 ggcgccagga ggtggaagaa gatccggtgc tgcccctcct tcacggcgcc gtgccactcc
     2701 agggtggtga tacccgaggc cgtgcgcgcc gccatcggat cggagctacg gatctcatag
     2761 gcggtggggc tgctgccagt cgcggcgggc gcgcccttgg ggcgcaggct gagcccggcc
     2821 aggccgatat tgcacttctc cgcgccggtg tgcctgccgg tcacctgaac gcgcaggcgg
     2881 tgctcgcccg gggcaagcga ctgctgcccg agcgacacgc gcatcggctg gacccccgag
     2941 gcataggtat cgatctcatc cccggcgggc ccgccatcga tcaggcagcg caccttgccg
     3001 cggtcactgt agccgaggaa gtcggcaaac acttcgccag agaacgcctc ttggagcgtg
     3061 aaagccatct ccagccactg cccggcctcc gtggcccgca gcaggacgat acccaggtcg
     3121 ttgaggcgca ccagatgctc cggccccgcc ggctcgctgg tgacacgggc ctgcagagcg
     3181 cggaccgcct gccacccggg cggcagtgcc gcagccgcgg gagccgggat gcggatcgcg
     3241 cccatctttg cctgccagcc accgccgctc gtctcccaaa gcgtttgcac ctcgatgttc
     3301 tcgctatccg tgcggaaagc aaggtcatca acgaccaggg catagcggcc aacgcgttgc
     3361 gccaggcttc ttcgccagtt gcagtaggcg gcgttgggta cctcgaccac ggcggcggcc
     3421 gt
//
LOCUS       DAOSCV010000453         3413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17683_length_3413_cov_3.502978, whole genome shotgun sequence.
ACCESSION   DAOSCV010000453 DAOSCV010000000
VERSION     DAOSCV010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17683_length_3413_cov_3.502978"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..145
                     /locus_tag="PLY56_11390"
                     /pseudo
     CDS             1..145
                     /locus_tag="PLY56_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
     gene            173..436
                     /locus_tag="PLY56_11395"
     CDS             173..436
                     /locus_tag="PLY56_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22129.1"
                     /translation="MTGLRVGAGIQLEKEAAARGRQPREHHAISGQRLVGDQVAPDEG
                     PPDIEPRVGVLLIVAPEGNEKLAQVPREAERKLLEGEIALLQL"
     gene            complement(1131..1517)
                     /locus_tag="PLY56_11400"
     CDS             complement(1131..1517)
                     /locus_tag="PLY56_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22130.1"
                     /translation="MRRAFSERKGFTRRLMALAVIHALLLAGALASYHQHGGPLAPAG
                     PSCHVGAPGMHPDESDHRDSMDGCLSCLWTRAAVGSQVAAGPAVVSLRAAQRVVSPDE
                     SGYRHIATLNLFSRGPPPLLSKRVDG"
     gene            1718..2065
                     /locus_tag="PLY56_11405"
     CDS             1718..2065
                     /locus_tag="PLY56_11405"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ22131.1"
                     /translation="MNRLVEPAVLFLLATGQATHGYEIITAANELYLTDAVIDAAAVY
                     RVLRDLEEQGCVVSSWSTEGSGPARRVYQITDVGRQRLAMWVQIIERRARQMQEFVER
                     YRASSVGQDAAAS"
     gene            2138..2509
                     /locus_tag="PLY56_11410"
     CDS             2138..2509
                     /locus_tag="PLY56_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ22132.1"
                     /translation="MRVAFAVDGGDVSAHFGHCAEYALVDIEGGKVVSQMRVPAPPHQ
                     PGILPPFLHRHGAGCVVAGGMGPRAVELFEQLGIQVVMGVQGRLDDAIAAFANGTLAG
                     GASTCGHEETACESPSDKCHE"
     gene            2522..2932
                     /locus_tag="PLY56_11415"
     CDS             2522..2932
                     /locus_tag="PLY56_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ22133.1"
                     /translation="MRVAVASTGEGLDAAIDPRFGRCACFVVVDTETMKATTIPNPGA
                     STAQGAGIQAAQSVANADVDAVIATNFGPNAHQALAAGEMALYSASGGSVRDAVDALQ
                     RGELKRVTAASVPAHFGMGQGGGMGRGGGRGGRW"
     gene            2929..>3413
                     /locus_tag="PLY56_11420"
     CDS             2929..>3413
                     /locus_tag="PLY56_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ22134.1"
                     /translation="MSRPARRSFTLAVAAGKGGTGKTLLATSLALALARADHGPVQIA
                     DCDVEEPNADLLLQPEVVRQVPVHVQVPRIDQEACIACGRCAQSCEFGALALIRCKVA
                     FFPALCIGCGTCAFICPAEAIAEEAREVGSIAVGKVGERLSFVQGRVHVGEQRVTPVL
                     QA"
BASE COUNT          575 a         1025 c         1230 g          583 t
ORIGIN      
        1 gatcatcacc caggccctca cctatgccct gatgctggcc ttcttccgca acgagatggg
       61 cttcggcggc aacaacggcc ttaccgactt caaggagctg ctgggcttca acctccaggc
      121 ggacggcatg cgcctcgccc tcttcgtggg cggcctcccc gtcggaatgt gagtgaccgg
      181 ccttcgtgta ggcgccggca tccagctgga gaaagaggcg gctgcgcgtg ggcgccagcc
      241 acgagagcac cacgccattt ccggtcagcg actcgtgggt gaccaggttg cgccagacga
      301 ggggccgccg gacatagagc cacgagtggg ggtgcttctg attgtcgcgc ccgaagggaa
      361 cgaaaaactt gcccaggtgc cccgtgaggc ggagaggaag ctgctcgaag gtgagatagc
      421 cctcctccag ctctagcttg aactcctcgt gtccgggggc gacaaggaag gcatcagcgc
      481 ggacatgcgg atagacataa ctctggatcc caatctccgc ctcgtcgagc cggaggcgat
      541 tgcggtcggg gtcgcggtca tcatcggata gcttgccgat ggcgctgcca acaaagctga
      601 tatcgggaag ctgcagatag cgcccgccgc gctgagccgg cgccgaggtt tcccccgcgg
      661 tctccgcttc cgcctctgcc tcggccggtg gaggcggagg cgcggtctcg gcctgagcaa
      721 gctgccagga tcctgctgtc aggaatgcag cgaacgccag ggatgcgatc atccgtgctt
      781 gagacatgcc aaactcctct tctctctgtt gcgccacgcc agcgagcctc acggcagccg
      841 cctaggccgg ggcgctgtgc cgtgggtttc ttgcctgtgc gcggttgaca aaccgccagc
      901 atggcatgcc gggttctctc cacggccccg cgcgatgccg cccggccagg aggatcgctc
      961 cgtgccagga gcctgcggcc caagggacgg gaccggctga ggcaaagagc ctgtgttctc
     1021 acgtttcagg cgccgagccg ctgcgaggcg tggaaaccgg caggatgccg ggaacgatgc
     1081 tgagtggagc gcgttaggat gggcgcacgg agacggtctg catcccgtct ctatccatcc
     1141 acgcgcttag agagaagagg aggggggccg cgggagaaaa ggttcagggt ggcgatgtgg
     1201 cgatagccgc tctcatcggg ggataccacc cgctgcgctg cccgtaagga gacgacggcg
     1261 gggccggctg ccacctggct gccaaccgct gcgcgcgtcc agaggcagga gaggcatcca
     1321 tccatcgaat cccggtgatc cgactcgtcc ggatgcatgc cgggcgcgcc cacatggcag
     1381 gaagggcccg cgggtgctag aggccctcca tgctgatgat aactggcgag cgcgcccgcg
     1441 agcaggaggg cgtggatcac cgccagcgcc atcaggcgcc gcgtgaagcc cttcctttcg
     1501 ctgaacgctc tccgcactat gcacctccga tacccgcgtg catctaccgt cattataggc
     1561 gcctcgcgtt cggctgtcaa ggcggccggc gcttgacatc aggtataatc ggtggtatga
     1621 tatctgcaag acacatatag tggaggttgg gatgccgaga ggccattgcc gaaagcatgg
     1681 agcgggatgc ccgccctggg cagccgggtt tgggagaatg aaccggctgg tggagccggc
     1741 ggtgctcttc ctgttggcga cggggcaggc gacgcatggc tacgagatca tcacggcggc
     1801 gaacgagctc tatctgacgg atgccgttat cgatgccgcg gcggtctacc gcgtgctgcg
     1861 cgatctggag gagcagggct gcgtggtctc ttcctggagc accgagggct ccgggcccgc
     1921 gcgacgggtc taccagatca ctgatgtcgg ccgacagcgg ctggcgatgt gggttcagat
     1981 catcgagcgc cgcgcccgac agatgcagga gttcgtcgag cgctaccgcg cctcctcggt
     2041 ggggcaggac gcagccgcct cgtgaaaggc gcgcgtctcg ggcctctttt tttctgcctc
     2101 aatatatgtg aatcacatct agagggagac aacagctatg agagttgcat tcgctgtgga
     2161 tgggggagac gtgtctgccc acttcggtca ttgcgcggag tacgccctgg tcgacattga
     2221 ggggggcaag gtggtcagtc agatgcgggt gccggcgccg ccgcaccagc cgggcattct
     2281 accacccttc ctccaccggc acggagccgg atgcgtggtc gccgggggca tgggcccgcg
     2341 ggcggtggag ctgttcgagc agcttggtat ccaggtggtg atgggggtcc aggggaggct
     2401 ggacgatgcc atcgcggcgt ttgcgaatgg aacgcttgcc gggggcgcga gcacctgcgg
     2461 gcatgaggag accgcgtgcg agtcgccatc cgataaatgc cacgaatgaa aggggagagt
     2521 gatgcgagtt gcggttgcct cgacaggcga ggggttggac gcggcgattg acccgcgatt
     2581 tggccggtgt gcttgctttg tggtcgtgga tacggagacg atgaaggcga ccacgattcc
     2641 gaatccaggg gcatccaccg cgcagggtgc ggggatccag gccgcacaga gcgtggcgaa
     2701 cgccgacgtc gatgctgtga tcgccacaaa cttcgggccc aacgcccacc aggcgctggc
     2761 ggcgggcgag atggccctct atagcgcgag tggtggcagc gtgcgcgacg ccgtggatgc
     2821 cctccagcga ggcgagttga agcgcgtcac tgccgccagc gtgccagcac actttggcat
     2881 gggccagggc ggcggcatgg ggcgtggggg cggaaggggc ggtcgctggt gagccgccct
     2941 gcgcgccgga gtttcaccct cgcggttgcc gccgggaagg ggggcaccgg caagacgctc
     3001 ctcgccacca gtctggcgtt ggcgctcgcg cgggctgacc atgggccagt ccagatcgcg
     3061 gattgtgatg tggaggagcc gaacgccgac ctgctccttc aaccggaggt cgtgcggcag
     3121 gttccggtgc atgtgcaagt gccgcgcatc gatcaagagg cgtgcatcgc ctgcgggcgg
     3181 tgcgcgcaga gctgtgagtt tggtgcgctg gcgttgatcc gctgcaaggt tgcgtttttc
     3241 ccagcgctct gcatcggctg cggcacgtgt gcattcatct gcccggccga ggcgatcgcc
     3301 gaggaagcgc gcgaagtggg cagcatcgcg gtcggaaaag tgggagagcg cctcagcttc
     3361 gtgcaggggc gggtccatgt cggcgagcag cgggtcacgc ccgtgttgca ggc
//
LOCUS       DAOSCV010000454         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17706_length_3410_cov_3.284650, whole genome shotgun sequence.
ACCESSION   DAOSCV010000454 DAOSCV010000000
VERSION     DAOSCV010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17706_length_3410_cov_3.284650"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3024
                     /locus_tag="PLY56_11425"
     CDS             <1..3024
                     /locus_tag="PLY56_11425"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ22135.1"
                     /translation="LLAVSEACNNAIQHGAGNIPGAEITVRIFTEPGGVHVEVSDPGA
                     GFDPGALWEPRSETPHGRGMLLMMQLTDKVEYTSDGHGTTVKLSKHACTGEEWDAEDG
                     SGRPGGRRRNRLRYVFDVCRTVSGTLELPRILAVVAERTARLFGVARARVLLHDPQRR
                     TATVAASWGERVRVRTEAATTPATRQAIYVAGEETQVGDLEPRPESEVQGHMVRSSIS
                     VPIVSRGEVVGSIHLDNFQRRRRFTNSDVMALRAIAAQAAVAIENARLYREAQRHVEE
                     LAAIGEVAHELNASLDLDHTLQTILDKTKDLLGIEVCAVMLLDAEGYLSVRASCGMTD
                     EYIGQQRALLEESPLARAITERRVIAAWDLRSYDASRGVYGGVVSAAAAPMMLHDRPI
                     GTLNIYTRERYRFSEDQLRVLRALADHAALAVHNAGLYETSQHARAQLETSMARTGSA
                     LHVASELHEVIRLVAELAAQALGMDGGALVLTDMGILAGAGEPSLTSEDAAAVRALQD
                     LASEVATNGEPLALGDVRERPQGHILRPAGIASFLAVPLATQEGLRGTLCVYARVPRA
                     LGGREVALLRSFAGQAAIVIENQRLLQDTNLRLEELGTLLEVGQSLLSRLDLEVVLDR
                     IIEHVSRIMHAQVCSILLVDRDAGDLYVAASRGLDVRHAGRLRMPYAEGVIGTVLRDG
                     CPRAIADIRRHSEFVYQDITAGQGLRALLCVPLATPTEVLGVLNVYRTTVHQWSDTEI
                     KFLSALATQAAIAIQNATLYQHEQQVAQALQKAFVPARPPAGSAVEIGRVYVPAGEHA
                     QVGGDYYDFIPLENGRLGIVIADVSGKGLRAATATAMGKYVLRAYVLENPAPGDAVVR
                     TNHALCHQLQEDGAFLTLFYALWDPRACRLTYVNAGHPHPFLWRRATGECVPLCCPRT
                     MLLGILADAQFAEKSIDIAPGDVLLAFTDGVSESRRGRELFGKERIRTAFEQVVNQPA
                     QVIADEIYRSVCEFRDGSAVDDITVLVVRFGGRPA"
BASE COUNT          538 a         1101 c         1159 g          612 t
ORIGIN      
        1 ctgctcgctg tctcggaggc atgcaacaac gccattcagc atggcgctgg taacatcccc
       61 ggcgccgaga tcaccgttcg tattttcacc gagccaggcg gggtccatgt ggaagtctcg
      121 gacccggggg cgggcttcga tccgggcgcc ctctgggaac cccgctctga aacccctcat
      181 ggccgtggca tgctcctgat gatgcagctc accgataagg tggagtatac gtcggatggg
      241 catggcacga ccgtcaagct gagcaagcac gcctgcactg gcgaggagtg ggatgccgag
      301 gatggctccg gccggccagg aggccgcagg cgcaatcgtc tccggtatgt cttcgacgtc
      361 tgccgcacgg tgtctggaac cctggagctt ccgcggatcc tcgccgttgt cgccgagcgc
      421 acagccaggc tctttggcgt agcacgtgcg cgcgtcttgc tgcatgatcc ccagcggcgg
      481 acggcaaccg tggcggcatc gtggggcgag cgagtccggg tgcgcacaga agcggccacc
      541 acgccagcga cacgtcaggc gatctacgtg gcgggcgagg agacacaggt gggggacctg
      601 gagccgcggc ctgagtccga ggtgcagggg cacatggtgc gctcctccat ctccgtgccc
      661 atcgtctcgc gcggtgaggt ggttggctcc atccacctgg acaacttcca gcggcggcgg
      721 cgcttcacca actcggatgt gatggctttg cgtgccattg ccgctcaggc cgccgttgcc
      781 attgaaaacg cgcgtctcta tcgcgaagcg caacggcacg ttgaggagct ggctgccatc
      841 ggcgaagtcg cccacgagct gaacgcctcg ctggatctcg accacacgct gcaaacgatc
      901 ctcgataaga caaaggacct actgggcatc gaagtgtgcg ccgtgatgct cctggatgcc
      961 gagggctacc tcagcgtgcg cgcttcctgc ggaatgaccg atgagtacat tgggcagcag
     1021 cgcgcgttgc tggaggagag cccgctcgcg cgcgctatca cggaacgccg tgtcatcgca
     1081 gcatgggacc tgcgctcgta tgatgcgtcg cgcggcgtct atggaggcgt ggtctcggcc
     1141 gcagccgcgc cgatgatgct gcacgaccgc cccatcggca cgctgaacat ctacacgcga
     1201 gagcgctacc gcttcagcga ggatcagctt cgcgtcttgc gcgcgctcgc ggatcatgcg
     1261 gccctggcgg tccacaacgc cggtctctat gagacgagcc agcacgctcg cgctcagctc
     1321 gagacgtcga tggctcgcac cggaagcgca ctgcacgtcg ccagcgagct gcacgaggtg
     1381 atccggctcg tggcggagct ggcggcccag gcgctgggca tggatggcgg cgcgctcgtg
     1441 ctcacggata tggggattct ggcgggcgcg ggtgagccat cgctgaccag cgaggatgcc
     1501 gccgccgtgc gagcgttgca ggatctcgcg agtgaggtgg cgaccaatgg cgagccgctg
     1561 gcgcttggcg acgttcgcga gcggcctcag gggcacatcc tccgaccggc gggaatcgcc
     1621 tcgttcctcg cggtcccgct tgccactcag gaagggctcc gcggaacgct gtgtgtctat
     1681 gcgcgtgttc cccgcgcgct cggggggcgc gaggtggcgc tcctccgctc ctttgctggg
     1741 caggccgcga ttgtgatcga gaaccagcgc ctgcttcagg ataccaacct gcgcctggag
     1801 gagctgggca ctctcctcga agtaggtcaa tcgcttctct cgcgacttga tctggaggta
     1861 gtgctcgacc gcatcattga gcacgtctcc cggatcatgc atgctcaggt ctgctccatc
     1921 ctgctggtcg accgcgatgc cggtgatctg tacgtggccg cgtcgcgcgg tctcgatgtc
     1981 cgccatgccg gtcgcttgcg catgccctac gcggagggcg tgatcggcac cgtcctgcgc
     2041 gacgggtgcc cgcgtgccat cgcggatatt cgccggcact ccgagtttgt ctatcaggat
     2101 atcacggcgg ggcaggggtt gcgcgcgctc ctatgcgtgc cgctggccac tcccaccgag
     2161 gtgctcgggg tactcaacgt ctaccgcacc accgtccacc agtggtctga tacggagatc
     2221 aagttcctga gcgccctggc gacccaagcc gccatcgcca tccagaacgc gacgctctat
     2281 cagcacgagc agcaggtggc gcaggcgctg caaaaggcgt tcgtgcccgc gcgcccgcct
     2341 gccggctccg cggttgagat cggccgggtt tacgtcccgg ctggcgagca tgctcaggtc
     2401 gggggcgact actacgattt tatccccctg gagaacggcc ggctcgggat cgtgattgcc
     2461 gatgtgagcg gaaagggcct gcgcgcggcg acggcgaccg cgatggggaa gtacgtcctg
     2521 cgtgcctatg tgctggagaa tcccgctccg ggcgatgccg tggtgcgcac caatcacgcg
     2581 ttgtgccacc agctccagga agatggcgcc ttcctcacgc tcttctacgc gctgtgggac
     2641 ccgcgggcct gccgcctgac ctacgtgaac gccggccacc cccatccttt cctctggcgc
     2701 cgtgcgacgg gcgaatgcgt tccgctctgc tgcccccgca cgatgctcct tggcatcctt
     2761 gccgatgccc aatttgccga gaagagcatc gatatcgctc ctggcgacgt gctcctggca
     2821 ttcacggatg gtgtgagcga atcgcgccga ggccgcgagt tgttcgggaa agagcgaatc
     2881 cgaacggcat tcgagcaggt ggtgaatcaa ccggctcaag tgatcgccga tgagatctat
     2941 cggagcgtgt gcgagttccg tgacggctcc gccgtggatg atatcacggt gctcgtggtg
     3001 cgctttggcg ggcgtccggc ctgatcgggt gtgatgaccg accccgcgag agtggcggct
     3061 ctctccgaac agacactcgc ttccggctca ctgcttcgga acgccggctg cagcgggcgc
     3121 gggccaacca cctgaacgcc gaacgggaac gttccgctct cccttgtctt acccacagac
     3181 ccctctccag cttctccggt gccgtggtgt agcgtgccgc ggtgcagagc cgattgagcg
     3241 cgcgggccta ccactgccgg agaggcaacg caagaagggc cgggggatgg tgagcgagaa
     3301 cctgaaggcg tggtatagcc gggtgcccgg ctgtgttgcc aagagaaggg cacgcctcgt
     3361 gcgccatccg tccgggggcg gcgggctgtt tgcctggcag gtgggggtgg
//
LOCUS       DAOSCV010000455         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17744_length_3405_cov_4.464478, whole genome shotgun sequence.
ACCESSION   DAOSCV010000455 DAOSCV010000000
VERSION     DAOSCV010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17744_length_3405_cov_4.464478"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLY56_11430"
     CDS             <1..222
                     /locus_tag="PLY56_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ22136.1"
                     /translation="YHHQIRVLLLAGFRHLALRVSDFDAAREHLASHGIAFTGEPSVA
                     AGGGRVLSFTDPEGNAVQIVSRPASFREP"
     gene            complement(282..1016)
                     /locus_tag="PLY56_11435"
     CDS             complement(282..1016)
                     /locus_tag="PLY56_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ22137.1"
                     /translation="MRKLLIMAHRGDQKHAPENTLAAFRKAIAIGADCIELDVRRTRD
                     GHFVVIHDERVDRTTDGTGRVADLLLAELRQLDAGSWFSPEYAGEQIPTFPEAIAAIA
                     PHLTFATEIKEPPGLIEAQVLATLKEAGALARTVITSFNADVIRRVKELEPSAKTGLI
                     GSDPGLLERAVAVGASWIDPYKDGCTPELVEAAHAADIRVTTWIIDTPEELRHYEAMG
                     VDRVTSNDPERLLREVGPLEEGVAAP"
     gene            1324..2046
                     /locus_tag="PLY56_11440"
     CDS             1324..2046
                     /locus_tag="PLY56_11440"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ22138.1"
                     /translation="MKVLITGAAGKVGRMVRRALIGHHAMRFLDVVPVDGTEGEMVVG
                     SILDRETLAAAMQGMDAVIHLAYGARNDMSLEAYEDLSFRVNAQGTFLVAQAAAAAGV
                     RRFVYASTLSLYEGNMPPAGEQFSEEHPPAPCGVYALTKYFGEEALRAYAREGKLSVV
                     SLRLTLPTEPEEWEAVRQQHPDSGRVRTAAVDVGQAFRLALEKPDVGFEVIHIASETP
                     GCPWDISKARRVLGYQPLVVFS"
     gene            complement(2109..3191)
                     /locus_tag="PLY56_11445"
     CDS             complement(2109..3191)
                     /locus_tag="PLY56_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOJ22139.1"
                     /translation="MRCTEADREAWNAFVAASPTGDVLQTWEWGELKARGAWEPIRIA
                     LRQDGAIVAVAGLLKRRLPIPGRCILYAPRGPVLDFSRDDLFRRMAAALRETGLRERA
                     LLVKIDPPVEDASVADRILAAGFVRSGQSREEGQGFGGEQPRCVMKLDLTPSLDDLLK
                     QCKEKTRYNIRLAGRKGVTIRVGESLEDMRTFYDLLLITAERDGFLVRSFDYYRDTWE
                     ILVQRGLARLFLAEYEGKPLAGAIAYLLGDKAWYTYGASSNEHRNVMPNYLMQWEMIR
                     WAKESGCSLYDFRGVSQSNDPTANDHLSGLNRFKAGFNATFVEYIGEFDLPLSSLGYW
                     AWRHGKPRAQAAMRGFARTLRRLGRR"
BASE COUNT          553 a         1130 c         1127 g          595 t
ORIGIN      
        1 tatcaccacc agatccgggt tctccttctg gcaggctttc gccacctggc gctgcgcgtc
       61 tccgatttcg acgcggcacg cgagcacctg gctagccacg gcattgcgtt caccggtgaa
      121 ccgagcgtag ccgcgggcgg cgggcgtgtc ctctccttca ctgatcccga ggggaacgcg
      181 gtgcaaatcg tctctcggcc ggccagtttt cgagagccgt aatcagaaca aggatggggc
      241 acattccgcc gggcaagagg cggtgagccc catcccgagt gctacggagc agcgacgccc
      301 tcctccagcg gcccaacctc tcgcaggagg cgctccgggt cattggaggt caccctatcc
      361 acccccatcg cctcgtagtg gcggagctcc tccggggtgt cgatgatcca cgtggtgacg
      421 cggatgtcgg cggcgtgcgc cgcctcgacc agctcgggcg tgcagccatc tttgtagggg
      481 tctatccagg aggcgcccac ggcgaccgcc cgctccagga gccccgggtc cgatccgatc
      541 agaccggtct tcgcggaggg ctccagctcc ttcacgcgcc ggatcacatc cgcgttgaag
      601 ctcgtgatca ccgtgcgcgc cagcgcgccg gcttccttga gcgtggccaa cacctgcgcc
      661 tcgatgaggc ccggcggctc cttgatctcc gtggcgaagg tgaggtgggg cgcgattgcg
      721 gcaatcgcct cggggaaggt gggaatctgt tcgccggcat actccggcga gaaccaggag
      781 cccgcgtcca gctgccgcag ctccgcgagc aacaggtcgg ccacgcggcc cgtgccatcc
      841 gtggtccgat ccacccgctc atcgtggatc accacgaagt gaccgtcgcg cgtgcgccgc
      901 acatccagct cgatgcagtc ggcgccaatc gcgatcgcct tgcggaatgc cgccagggtg
      961 ttctccgggg catgcttctg gtcgccccga tgggccatga tcaggagttt gcgcatcttt
     1021 gcctcgactt cccggccggc aggcgccgcc ggcccttctt ccaagccttc cagccttccc
     1081 cttccgtacc cgcggcccga gttcctctct gccagaaacg gtgccggaga acatgcccgc
     1141 cggcgccccg attgcccgct ccacgctgat acgccgtttt ggccccgctt ctgctccccc
     1201 gccacggtgg gcgggccgcg tgccgcccga ttcaaggcaa cccggaaggc tgttccgtga
     1261 ggaaaagccg cgcaccgcga tgggcttccc cgggcggcga tcgtgtctgc gggagggtgg
     1321 aggatgaagg tattgatcac cggcgcggcc ggcaaggtcg gtcgcatggt acggcgtgct
     1381 ttgatcgggc accacgcgat gcgctttctc gacgtggtcc cggtggatgg caccgagggc
     1441 gagatggtgg ttgggagtat actcgaccgc gagacgctgg cagccgcgat gcaggggatg
     1501 gatgccgtga tccatcttgc ctatggcgcc cgaaacgaca tgtctctgga ggcgtatgag
     1561 gatctctcct tccgggtcaa tgctcagggc acgtttctgg tggcgcaggc ggcggccgcc
     1621 gcgggcgtgc gccgcttcgt ctatgccagc acgctgagcc tctatgaagg gaatatgcct
     1681 ccggcgggcg agcagttcag cgaggaacac ccgccggcgc cgtgcggcgt ctacgcgctc
     1741 accaagtact tcggggagga ggcgctccgc gcgtacgctc gcgaagggaa gctctccgtg
     1801 gtctcgctgc gcctcacgct gccgaccgag ccggaggagt gggaggctgt ccgccagcag
     1861 catccggatt ccgggcgcgt gcgcaccgcc gcggttgatg tgggccaggc gttccggctc
     1921 gccctggaaa agcccgacgt cggcttcgag gtgatccaca tcgcgagcga gacgcccggc
     1981 tgcccctggg atatcagcaa ggcgcggcgg gtcttgggat accaaccgct cgtggtcttc
     2041 tcgtgagcaa gctgtggttg aacgggcgga atgtgccctc tttccgcccg ttcagccgcg
     2101 ggtttcggct agcgccggcc aaggcggcga agcgtccggg caaagccgcg catggccgcc
     2161 tgggcgcggg gcttgccgtg gcgccatgcc cagtagccca aggaggagag aggcagatca
     2221 aactcgccga tgtactcgac gaaggtggcg ttgaagccgg ccttgaagcg attgagcccg
     2281 gagaggtggt cgttcgcggt cggatcgttg gattgcgaga cgccgcggaa gtcgtagagg
     2341 gagcagccgc tctcctttgc ccagcggatc atctcccact gcatcaggta gttgggcatc
     2401 acgttgcggt gctcgttgct cgaggcaccg taggtgtacc aggctttgtc acccagcaga
     2461 taggcaatcg ccccggcaag tggctttccc tcgtactcgg ccaggaaaag acgcgccagg
     2521 ccacgctgga caaggatctc ccaggtatcg cggtaatagt caaagctgcg cacaaggaag
     2581 ccatcgcgtt cggccgtgat caggagcaga tcatagaagg tgcgcatatc ctctagagac
     2641 tcgccgacgc ggatggtcac ccccttgcgt ccggcgaggc ggatgttgta gcgcgtcttc
     2701 tccttgcact gcttcagcag gtcatcgagg gatggagtca ggtcgagctt catcacgcag
     2761 cggggctgct cgccaccgaa gccctggccc tcttcccggc tctggccgga gcggacgaag
     2821 ccagcggcca ggatccgatc cgcgacgctg gcatcctcca ccgggggatc aatcttgacc
     2881 aggagagcgc gctcacgcag gcccgtctcg cgcagggccg cggccatgcg acggaagagg
     2941 tcgtcgcgtg agaagtcgag cacgggcccg cgaggggcgt agaggatgca ccggccggga
     3001 ataggaagcc ggcgcttgag caaacccgcg accgccacga tggcgccatc ctggcggagc
     3061 gcgatacgga tcggctccca ggcaccgcgc gctttcagct ccccccactc ccacgtctgg
     3121 agcacgtctc ccgtgggaga agccgccaca aaggcattcc acgcctcgcg gtctgcctct
     3181 gtgcaccgca cgatctgaag ctctgcccct cgctcttccg ccacgcacgc acctgccttt
     3241 ccggtcaacc cagggctatt ttaccgcaga ggcagaagcg agtcaaactt gagtgcgtat
     3301 tgacacggcc ccgtcacttt gttatactct caacaggcaa ggccgggatt cggccgacta
     3361 tctggggcgg gaagcttgcg gcaatctcag cgttgtgctt tccgc
//
LOCUS       DAOSCV010000456         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17749_length_3404_cov_4.041804, whole genome shotgun sequence.
ACCESSION   DAOSCV010000456 DAOSCV010000000
VERSION     DAOSCV010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17749_length_3404_cov_4.041804"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1077)
                     /locus_tag="PLY56_11450"
     CDS             complement(103..1077)
                     /locus_tag="PLY56_11450"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ22140.1"
                     /translation="MDNETLLELLRCHSTPGDEGEVAAVLAREWLDAGWRVTRHGAYA
                     ISARCPGGASRKPRLLITAHMDSPGFTVERRERKSLYVLPLGGAGFQGKSTPAILKTA
                     GGKVPVRLHCKADKEGRRTYHFRDPGTPIRHGDRVCFAAEPTISRKGEITSPFLDNRL
                     GCGLLCEVARALPADGLAFEVVLGATAAEEFGGFGATVLARAIEPDLVICLDATYESP
                     EQRVLLGKGPVLTLSDASVLISPAMRDLVLEWFHRRGLLLQTEVYNVSGTDARAFPKV
                     GLPAPVLALLIATRGNHSPAEVANLADYASLREVLLRFAVDPPPLLEA"
     gene            complement(1084..2073)
                     /locus_tag="PLY56_11455"
     CDS             complement(1084..2073)
                     /locus_tag="PLY56_11455"
                     /inference="COORDINATES: protein motif:HMM:NF025673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4380 domain-containing protein"
                     /protein_id="HOJ22141.1"
                     /translation="MTRGMFLLAAVMLGMLAAPVAAREYPGTAMVEFAGYTRCIELRN
                     ETTRVVLGPHCGGRVLSYSMEGREALYRDPNHDGWTYTPGKRTVDICGGRLDIGPEMT
                     IPRHPILWLGAWKAEITGSRAARMTSQPDPSTGVQLVRDFRLAAKGSRLTVRQTIRNV
                     SKETKHWCHWSRTLAAPGGIFVIPLNPQSRFPKGYLLYGPGPVMNYSPAEEPAIRRRD
                     GFLEVRAVPSQPKFGIDSMAGWMAYAMPNDLLFVKRFPVYPNRVYSEMAAFTVSLYYV
                     PQFTELEPIGPRETIRPGKSASYTEEWELIPFSFPSEGEEIDLKRVEALAKAK"
     gene            complement(2187..>3404)
                     /locus_tag="PLY56_11460"
     CDS             complement(2187..>3404)
                     /locus_tag="PLY56_11460"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ22142.1"
                     /translation="EGYMELFRQNLREHRCSGTSYIGGITAKREGDTVVVDTSKLKET
                     ADENIRRYGFQVFNVPAIFLGDASGLYAKDKKWQGFEVFTPEFDRAFESYCKQVGDAL
                     RAEGLLPYALWQIWDEPQNREMKEMCIHLARLVKKAVPDARIYLTAGVEDELLDWVDI
                     WNLPWPSTYSSEAAAKVRAKGASLWAYENDLYSLDVMDSSLRMRAFPWRLRRYGIEGV
                     EWWAISQWKSDPWTVPNQYAPQNGGGFFLYPTKDRKGAPIDSIRWELYREGVEDYDLL
                     TLFAEEQDRVLKALGVSDTRLSGQAQMLELVSRVALSTVDATDDPRVIEETRRAVAER
                     VEFLRRAPAAVAGFVTGAKGTTLLVTAEKGARVVVDGKPQTGAMISVPVKPGQPVRVE
                     VTRGKATKRIVLR"
BASE COUNT          584 a         1136 c         1102 g          582 t
ORIGIN      
        1 aagggctttg ccggaaagca gcgcggagct tcgagctccg cgcgtgcgcg cggccttctc
       61 cttatgtcgg aatcccaggg gggcaagcct cgcccctggt gattatgctt ccagcagcgg
      121 cggcgggtcc acggcgaagc ggagaagcac ctctcgcagg gaagcgtaat ctgccagatt
      181 cgccacttcc gcgggcgagt ggttgccgcg cgtggctatg aggagcgcga gcacaggcgc
      241 gggcaacccc actttcggaa aggcgcgggc atcggtgccg ctgacgttat acacctcggt
      301 ttgcagcagc aagccccgcc ggtggaacca ctccaggacc agatcgcgca tcgccgggct
      361 gatgagaaca gacgcatcgg agagcgtcag caccggcccc ttccccagca acacccgctg
      421 ctccgggctc tcgtaagtgg catcgaggca gatgaccagg tccggctcga tggcgcgcgc
      481 gagcaccgtt gccccaaagc cgccgaactc ctcggctgcc gttgccccca agaccacctc
      541 gaaggcgagc ccatccgccg gcaatgcccg ggcgacctcg cagaggagcc cacatcccag
      601 acggttgtcc agaaacggcg acgtgatctc gccctttcgt gagatcgtgg gttcggcagc
      661 gaagcagaca cggtcgccat gcctgatggg cgtgcccgga tcacggaagt ggtaggtgcg
      721 gcgtccctcc ttgtcggctt tgcagtggag gcggaccggc accttccccc cggcggtttt
      781 caggatggcc ggcgtggatt ttccctggaa gccggctccg ccgagcggca gcacatagag
      841 gcttttcctc tcgcggcgct ctacagtaaa gcccggcgag tccatgtgcg cagtgatcag
      901 caagcgtggc ttgcgcgacg cgccgcccgg gcaccgggcc gaaatggcat acgccccgtg
      961 cctcgtgacc ctccatccgg catctagcca ctctcgcgca agcacagcgg ccacttcgcc
     1021 ctcatcccca ggcgtgctgt ggcagcgcag caactccagc agcgtctcgt tatccatcgc
     1081 gacctacttc gctttcgcca gggcttcaac ccgcttcagg tcgatctcct cgccttccga
     1141 tgggaaggag aaggggatca gctcccactc ctcggtgtag gatgcggatt tgccggggcg
     1201 gatcgtctcg cgcgggccga ttggctccag ctcagtgaac tggggcacgt agtagagtga
     1261 gacagtgaag gcggccatct cgctgtagac gcggtttggg tagacgggga agcgcttcac
     1321 gaaaagaaga tcgttcggca tcgcgtaggc catccagccg gccatggagt cgatcccgaa
     1381 cttgggctgc gacggcaccg cgcgcacctc caggaagcca tcgcgccggc gaatggccgg
     1441 ctcctcggcg ggagagtagt tcatcaccgg gccgggacca tacaggaggt atccctttgg
     1501 aaagcggctc tgcggattca ggggaatcac gaagatcccg ccaggcgcgg cgagcgtccg
     1561 gctccaatgg caccagtgct tcgtctcctt cgagacgttg cgaatggtct ggcgcacggt
     1621 gagacgcgag cccttcgcgg ccagccggaa gtcgcggacg agctgcacac ccgtggaagg
     1681 atccggctgg ctggtcatgc gggcggcacg ggaaccggtg atctcggcct tccaggcccc
     1741 cagccacagg atggggtgcc tcgggatcgt catctcgggg ccgatatcaa gccggccgcc
     1801 gcagatatcc accgtccgct tcccgggagt gtaggtccag ccgtcgtggt ttggatcgcg
     1861 gtagagcgct tctcggccct ccatcgagta gctcagcact cttccgccgc agtgcggccc
     1921 gaggaccacg cgcgtggttt cgttgcggag ctcgatgcac ctggtgtagc cggcgaactc
     1981 aaccatcgcc gtgccaggat actcccgggc tgccacggga gcggccagca ttcccagcat
     2041 caccgccgcg agaagaaaca tgccacgggt catgagcttc tccgagagga gagatcgcgc
     2101 cgggcgcctg ccagctgggg gctcgttgcc tcaccaacgg aagggcgccc ggcgatgcaa
     2161 ccttatcggt cgcccgaggg gctctcttag cgcaagacga tccgcttcgt cgccttgccc
     2221 cgggtgacct cgacacgcac gggctggccg ggcttcaccg gcaccgagat catggcgccg
     2281 gtttggggct ttccgtccac gacgacgcgc gcgcccttct ccgcggtgac caggagcgtg
     2341 gtgcctttgg caccggtgac gaagcccgcc accgccgccg gggcccggcg caggaactca
     2401 acacgctccg ccacagcgcg ccgtgtctcc tcgatcacgc gcgggtcgtc cgtcgcatcc
     2461 accgtgctca gggcaacccg cgagacgagc tcgagcatct gcgcttggcc ggaaagccgg
     2521 gtgtcggaaa ctccaagcgc cttcagcacc cggtcctgct cctccgcgaa gagggtcaag
     2581 aggtcgtaat cctccacgcc ttcgcggtag agctcccagc gaatgctgtc gatcggcgcg
     2641 cccttgcgat ccttcgtcgg gtagaggaag aagccgccgc cgttctgtgg cgcgtactgg
     2701 ttgggcacgg tccaggggtc gctcttccac tggctgatgg cccaccactc gacgccttcg
     2761 atgccgtagc ggcggaggcg ccacgggaaa gctcgcatcc gcagggagga atccatcacg
     2821 tcgaggctat acaggtcatt ctcgtacgcc cagagggaag cgcccttggc acgcaccttg
     2881 gcagccgcct ccgaggagta ggtgcttggc cagggaaggt tccagatatc gacccaatcg
     2941 aggagctcgt cctccacgcc cgcggtcagg tagatgcgcg cgtcgggaac ggccttcttc
     3001 accaggcggg cgaggtggat gcacatctcc ttcatctcgc ggttttgcgg ctcgtcccag
     3061 atctgccaga gggcataggg gaggagacct tcagcgcgga gagcgtcgcc cacctgcttg
     3121 cagtagcttt caaacgcccg gtcgaactcc ggggtgaaca cctcgaagcc ctgccacttc
     3181 ttatccttgg cgtagaggcc gctcgcgtcg cccaggaaga tggcgggcac gttgaagacc
     3241 tggaacccgt agcggcggat gttctcgtcc gcggtctcct tcagcttgga ggtatcgaca
     3301 actaccgtat cgccctcacg tttggccgtg atcccgccga tgtagctcgt gccagagcaa
     3361 cgatgctcgc gcaggttctg ccggaagagc tccatgtaac cctc
//
LOCUS       DAOSCV010000457         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17838_length_3392_cov_3.454001, whole genome shotgun sequence.
ACCESSION   DAOSCV010000457 DAOSCV010000000
VERSION     DAOSCV010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17838_length_3392_cov_3.454001"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="lepB"
                     /locus_tag="PLY56_11465"
     CDS             complement(<1..358)
                     /gene="lepB"
                     /locus_tag="PLY56_11465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ22143.1"
                     /translation="MAWKRWLVVLGVVLGVRLLNPVNVAVVDGRSMEPAMHDGQLFLI
                     DRLRYRFVPVARGDVVAFHHGNEVFIKRVAAIGGDELWVLVSPDGEHIEWIPHHELAR
                     MRVLVARHPMLGRVERR"
     gene            1181..1492
                     /gene="rplU"
                     /locus_tag="PLY56_11470"
     CDS             1181..1492
                     /gene="rplU"
                     /locus_tag="PLY56_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ22144.1"
                     /translation="MYAVVHTGGKQVRVAPNDVVDVEKLETAVGEQVTLDRVLMVRTE
                     DATRVGTPFVEGAKVVCRVLEQGRGEKIRGFTYKPKKNIRRRFGHRQAYTKLLVEEIQ
                     A"
     gene            1519..1770
                     /gene="rpmA"
                     /locus_tag="PLY56_11475"
     CDS             1519..1770
                     /gene="rpmA"
                     /locus_tag="PLY56_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ22145.1"
                     /translation="MAHKKGMGSSRNGRDSQPKYLGVKESDGTTVRPGSIIVRQRGTH
                     FKPGRGVGLGKDHTIFALVEGVVRFEGEGKTRKVSVLNA"
     gene            1891..3279
                     /gene="obgE"
                     /locus_tag="PLY56_11480"
     CDS             1891..3279
                     /gene="obgE"
                     /locus_tag="PLY56_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOJ22146.1"
                     /translation="MFIDEATLHVKAGRGGNGITAFRREKFVPRGGPSGGNGGRGGDV
                     ILEVDSNKNTLLDFQNRQLYQAENGKNGGPSNKQGSNGASIVLRVPPGTMVYDAATGE
                     LVADLTGEPGSERVVIARGGRGGRGNAAFATARNQAPHFAENGEPGEERELRLQLKLL
                     ADVGLVGFPNAGKSTLISRVSAARPKIADYPFTTLVPQLGVVRVGDWKSFVMADIPGL
                     IEGAHAGAGLGHQFLRHIERTRILIHILDVSGMTGRDPVDDYHTINRELAAYNERLAS
                     LPQIIALNKVDLPGSEEIAERTRIALEPEGRAIFAISAVTGQGVPALIAAAAEALEQA
                     RAEAPAPGVGVEEEEEPPVYQPPRTPRAQPWSVHRGEDHTFVVQGKGVERMVAMTDLQ
                     HEGSLRALQKKLDRMGVFDRLRAEGAQDGDTIRIGDLEFDFVEEEQLYERLERAAMSR
                     RARREQRESEEK"
BASE COUNT          641 a         1060 c         1148 g          543 t
ORIGIN      
        1 cccgccgttc gacgcgcccg agcatcgggt ggcgcgccac gaggacgcgc atacgcgcca
       61 gttcgtggtg ggggatccac tcgatgtgct cgccatcagg cgagaccaac acccacagct
      121 cgtcgccgcc aatggcggcc actctcttga tgaagacctc gtttccgtgg tggaaagcca
      181 ccacatcacc ccgcgccacc ggcacgaacc gatagcgcag gcgatcgatg aggaagagct
      241 ggccgtcgtg catcgccggc tccatggagc ggccatccac cacggccacg ttcaccgggt
      301 tgagaagacg cacgcccagc accaccccca gcacgacgag ccaccgtttc cacgccaaag
      361 ccatttcccc cgcgaacccc acccacctga tgcagttgtc gtgagcgatc agggggagag
      421 cttcgcgatg cccgccggga acaagagcgc cgatcttgcc ccagcgctcg ccggcgattg
      481 gcgttggtca cctacccgcg gcaacaggca tgagccgcgt gctgcctggt ctctcccgtc
      541 ctgatcgcct gctcgccggc agcttatccg gtggcgatct ccctgcccat gggatctcgc
      601 tcgcggacgc aacggcgtga caccggcggc agcacggttc atgccatccg gggttccttc
      661 ggccagaaga ggatcacgcg cctggggcgg cggccgctga tggaggtgcg agaggggcgt
      721 tctgggtgcc tgtctacgaa cgatggggcg cccgcggggg agccccatcg tttcgacaga
      781 tcccactatc cagaaggagg taccatgctc taccgctgat cagtctaccc acggctcccc
      841 ggatccaaac atgcgcgctc ataacattca cggtgtttcg gccatgcgca ggctgggagc
      901 cgtcacctag aaaagaggcc cagggcgggt ctgccagacg atcgccgggc ccatcgtcac
      961 cgcttcgcgg gtgggcacgg tttcggcgcc gatggggtgc gcgccgccgc atcacccgag
     1021 agctccagac accttcagat ccgagcaaca gcctgaaatc ggccttgaca gacggagcct
     1081 cctgtggtaa gatggtggcg aaaagcgcac tcatgatgat ccagcgcgac ggtggcgagg
     1141 ccatcgccgc gactttttgt gaaggggttg tgacgaggcg atgtacgctg tagtgcatac
     1201 gggcggcaaa caggttcggg ttgcccccaa tgatgtggtt gatgttgaga agcttgaaac
     1261 ggcggtcggc gagcaggtga cgctggatcg tgtgctgatg gtccgcaccg aggacgcgac
     1321 gcgcgtcggc acccccttcg tcgagggcgc caaggtagtg tgccgcgtgc tcgagcaggg
     1381 ccgcggcgag aagattcggg gcttcaccta caagccgaag aagaacatcc ggcggcgctt
     1441 cggccaccgc caggcataca cgaagctcct tgttgaagag atccaggctt gaggtcgggt
     1501 caggaggcga agtagtccat ggcacacaaa aagggtatgg ggagctcccg caacggccgg
     1561 gatagccagc cgaagtatct gggagtgaag gagagcgacg gcaccacggt gcgccccgga
     1621 agcattattg tccgccagcg tggcacccac ttcaagcccg ggcgcggcgt tggtcttggg
     1681 aaagaccaca ccatcttcgc gttggtcgag ggcgtggttc gcttcgaggg cgaaggcaag
     1741 acccgcaagg tttctgtgct gaacgcttga gcgcattggt ggcgcacatt tccggggagc
     1801 gtccgcgcat gcggcgctcc ccgagctact ctgcatccgt tcggttcctc cgcgctcatt
     1861 cccggcccat actctgggta gagtgtacgt atgtttatcg atgaagcaac actccatgtc
     1921 aaagccggta gaggtgggaa tgggattacc gcgttccggc gggagaaatt cgtcccccgc
     1981 ggcggaccct cgggtggaaa cggcggccgg ggcggcgacg tcatcctcga ggtagacagc
     2041 aacaagaaca cgttgctcga cttccagaac cggcagctct accaggcgga aaacgggaag
     2101 aacggcggcc cctcgaacaa gcaagggagc aacggcgcct ccatcgtttt gcgtgtgccg
     2161 ccgggcacga tggtgtatga tgccgcgacc ggcgagctgg tggccgatct gacgggcgag
     2221 ccaggcagcg agcgcgtggt gatcgcgcgc ggcgggcgcg gcgggcgcgg aaatgccgcc
     2281 ttcgccacgg cacgcaacca ggcgccgcat ttcgccgaga acggagaacc tggcgaggag
     2341 cgcgagttgc gcctccagct caagctcctg gcagatgtcg gtttggtcgg tttccccaat
     2401 gccggcaaat cgacgctgat ctcccgcgtt tcggcggcca ggccgaagat cgccgactac
     2461 cccttcacga cgctcgttcc ccaactcggg gtggtgcgcg tgggtgactg gaagagcttt
     2521 gtcatggccg atatcccagg gctcatcgag ggggcacacg cgggtgccgg cctgggtcac
     2581 cagttccttc gccatatcga gcgcacgcgc atcctcatcc acatcctcga tgtctcgggc
     2641 atgaccgggc gcgacccggt ggatgattac cacacgatca accgcgagct ggctgcctac
     2701 aacgagcgac tggcctccct gccccagatc atcgccctga acaaggtgga cctgcctggg
     2761 tccgaggaga tagcggagcg gacgcgaatt gccctcgagc cggaggggag agcgatcttc
     2821 gcgatctcgg ccgtcacggg ccagggcgtg ccggcgctca tcgccgcggc ggccgaggcc
     2881 ctggagcagg cgcgcgcgga ggcgcctgca cccggcgtgg gcgttgaaga ggaagaagag
     2941 cccccggtgt atcaaccgcc gcgcactcct cgggcgcaac cgtggtccgt gcatcgcggc
     3001 gaagaccaca ccttcgtggt gcaaggcaag ggcgtcgagc gcatggtggc tatgaccgac
     3061 ctacagcacg agggaagcct gcgtgccttg cagaagaaac tggatcgaat gggcgtcttc
     3121 gaccgcctgc gcgcggaagg cgcccaggat ggcgatacaa tccgcatcgg ggatctggag
     3181 tttgatttcg ttgaggaaga gcagctctac gagcgcctgg aaagggcagc tatgagccgg
     3241 cgggcccgcc gcgagcagag ggagagcgaa gagaagtgat gacggttcgc agggcggtgg
     3301 tgaaggtggg gacaagcacc ctcaccggag gcacgggagt tctagacgag gcctatatcg
     3361 aggacctggt gcgcgatctg gcacgggcgc gc
//
LOCUS       DAOSCV010000458         3383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17905_length_3383_cov_2.928786, whole genome shotgun sequence.
ACCESSION   DAOSCV010000458 DAOSCV010000000
VERSION     DAOSCV010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17905_length_3383_cov_2.928786"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLY56_11485"
     CDS             <1..625
                     /locus_tag="PLY56_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ22147.1"
                     /translation="PGYLDPDQDAALTDQVLRLSDGRVTSATLRGVRDTRDLAINPHR
                     GAFYSVSTELAGGPLGGTWSFGKIGVDLRRYFPIGKAKPDGSNQKVLATRLMAGASIG
                     KMPLSEHYWLGGAETLRGYREDEFNGTRMLLLSTEFRVPLGTSLQGVTFFDCGYAWPR
                     NQGLRLGELKPAVGLGLRVITPLGPLRLDYGFGTDGGRSHFSIGHVF"
     gene            831..1511
                     /locus_tag="PLY56_11490"
     CDS             831..1511
                     /locus_tag="PLY56_11490"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOJ22148.1"
                     /translation="MFRRFCLLALVALFAGAGRTTAVAQAAAGEQPAAVSIGVVDMER
                     VSEDSLPYKEANKELQALQQSLNQNIQEVAQLTYLTGPETNELVAILDAKELTAAQKA
                     RHAELKKTADEREKEFSTLMQTKNPTDQQKKRLQELNAMRNGREQILAELNQKYQQSF
                     RQKLQEVDAQLGKSVSEIINEVAKARKLSIVLSKSVIITLDRKQDVIFFGGTDITDEV
                     IKRLNQRK"
     gene            1570..2658
                     /locus_tag="PLY56_11495"
     CDS             1570..2658
                     /locus_tag="PLY56_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22149.1"
                     /translation="MKWVWAVAAISLACAGCKPQASALPPPTACRVDLTRLERVHPRW
                     QELRSLDERIREVRERLQAGVAPSAAAPLPAPLPPVPHPADPAERAVPESLEEMAAAR
                     DAAVRAQQNALAERHKARLERERLEREAEKEFLVKQQRTLAFEELWQEQNAVIRRYQP
                     EITRLRVLIARLRDPSPVPDPEKRRVAELDQRIQELTRLERQRNAALEAVAKRVEERL
                     AQEMARRNQEDTAALEALRAELKSQQEMAIRTAEQDWQDRIDAVAEALREPPPGPVPA
                     GVREDAPPVAARTEITPAPDSAQAADAAGATLARQRTRLYRLIQAETRQRVLGIAARH
                     NLRPVFDGGTRLPDRTQFFLRELQHPGL"
     gene            2766..>3383
                     /locus_tag="PLY56_11500"
     CDS             2766..>3383
                     /locus_tag="PLY56_11500"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOJ22150.1"
                     /translation="MSTTLREIAALVGGTIVGDADTLVAGAAGLEDAAQGDILFVENR
                     RYAERAAASQASAVLVPPDVVIPNKPCITVEDPRAAFLKVLSFFQPRERLPQGIAETA
                     RIGQNVTLGEGVAIGDYCCIGDNVVLGDGVVLFPMVYVGDNVTIGPNTVIYPQVTVYR
                     DVTIGARVRIHSGSVIGADGFGYIFVEGQHVKVPHIGTVTIEDDVE"
BASE COUNT          638 a         1076 c         1123 g          546 t
ORIGIN      
        1 tccaggctac ctggatcccg atcaggatgc cgcgctgacc gaccaggtgc tcaggctctc
       61 ggatgggcgc gtcaccagcg ccacgctgcg cggagtgcgc gacacgcgtg acctggcgat
      121 caacccgcac cgcggtgcgt tctacagcgt ctccaccgag cttgccggcg ggccgttggg
      181 cggcacctgg tccttcggaa agataggggt ggacctgcgc cgctacttcc ccatcggtaa
      241 ggcgaagccg gatggcagca accagaaggt gctggccacc cgcctgatgg ccggcgcctc
      301 gattgggaag atgccgctct cggagcacta ctggctcggc ggcgccgaga cgctgcgcgg
      361 ataccgcgag gacgagttca acggcacgcg catgctcctc ctcagcaccg agttccgcgt
      421 tccgctagga accagcctgc agggcgtgac cttctttgac tgtgggtacg cctggccgcg
      481 aaaccagggt ctgcgcctgg gcgagttgaa gcctgccgta ggccttggcc tgcgcgtgat
      541 tacgccgttg gggcccctcc gcctcgacta tggctttggc accgatggcg ggcgcagcca
      601 cttcagcatc ggccacgtct tctagcggag ataggtgctg ttctggtata ataagccatt
      661 atcggaccag gaaagggcgg aatcgccgcg cgccgagtgg ggccgcgcat ccaccctgcc
      721 cccgtcagga gggtctcgcg gcactgcttc gaagcaacta cccgacgacg gcacaagccg
      781 ccgcccgaaa cggctcatca tggtgagctg atagtaaagg agatacttct gtgttcagac
      841 gcttctgtct cttggcactg gtcgccctgt tcgccggggc tggccgcaca accgctgtgg
      901 cacaggcagc cgccggcgag cagcccgcgg ctgtcagcat cggtgtggtg gatatggagc
      961 gagtgagcga ggactcgctc ccttacaagg aagccaacaa ggagttgcag gcactgcagc
     1021 agagcctgaa tcagaacatt caggaggtcg cgcagctcac ctacctgacc ggcccggaga
     1081 caaacgagct tgtggccatt ctcgatgcca aggagctgac cgcggcgcag aaggcaaggc
     1141 atgcggagct gaagaagacg gccgatgagc gcgagaagga gttcagcacg ctcatgcaga
     1201 ccaagaaccc gacggatcag caaaagaagc ggctgcagga gctgaacgcc atgcgcaacg
     1261 gccgcgagca gatcctggcc gagctgaacc agaaatacca gcagagcttc cgccagaagc
     1321 tgcaggaagt cgatgcccag ctcggcaagt cggtgagcga aataatcaac gaggtagcca
     1381 aggcccgcaa gctctcgatc gttctctcca agtcggtgat catcacgctc gaccggaagc
     1441 aggacgtcat cttctttggt ggcaccgaca ttaccgacga agtgatcaag cgcctgaacc
     1501 agcgcaagtg atgccgaacg gcgggcagcg cgcgagatgg caggcggcgc gcgaggcagg
     1561 aggcctgtgt tgaagtgggt ctgggcagtc gcagcgattt ccctggcttg tgccgggtgt
     1621 aagccgcagg cctctgccct tcccccgcct accgcctgcc gggttgacct gacgcgtctg
     1681 gagcgggtcc atccgcgctg gcaggagctc cgctcactag acgagcgcat ccgggaagtg
     1741 cgagagcggc tgcaagcagg tgtcgctccc tcggctgccg ctcctcttcc cgcgccgctt
     1801 cctcccgtgc cccaccctgc cgatccggca gagcgcgccg ttccggaatc gctcgaagag
     1861 atggccgcgg cgcgtgatgc cgccgtgcgc gcccaacaga atgctttggc agagcgtcac
     1921 aaggcacgcc tcgagcggga gcgcttggag cgcgaggcgg agaaggagtt tctggtcaag
     1981 caacagcgca cactcgcgtt cgaggagtta tggcaagaac agaacgcggt catccgtcgc
     2041 tatcagccag agatcacccg gctccgcgtc ctgatcgctc gccttcgtga cccgtccccc
     2101 gttcccgatc cggaaaagcg ccgtgtcgcc gagctggacc agcgcatcca agagttgact
     2161 cgcctcgagc ggcagcgcaa cgccgccctg gaagcagtgg caaagcgcgt tgaggagcgg
     2221 ctggcccagg agatggcgcg gcgcaatcag gaagataccg ccgcgctaga ggcgctccga
     2281 gccgagctga aaagccaaca ggagatggcc attcggaccg cggaacaaga ctggcaggac
     2341 cgcatcgatg cggtcgccga ggcgcttcga gagccgcccc cggggcctgt ccccgctggc
     2401 gttcgagagg atgcgccgcc tgtcgcggct cgcaccgaga tcaccccggc acccgattcg
     2461 gctcaggcgg cagatgctgc cggcgccacc ctcgcgcgcc agcgaacccg cctctaccgc
     2521 ttgatccagg ccgagacacg gcagcgggtg ctcgggatcg cggcccggca taacctccgc
     2581 ccggtgttcg atggtggcac gcgcttgcca gaccgaacac agtttttttt gagagagctc
     2641 cagcatccgg gcctgtgagc agtggcaccc cctccccagg gagggcagac gcgcctcgaa
     2701 ggcagcttct tgcttcgcga gctgcccctg gaggcgcgcg tgatgatcag tgttgaggtg
     2761 agaacgtgag tacaacgctc cgggagatcg ctgccctggt gggcgggacg attgtgggtg
     2821 acgcagatac gctggtggcc ggagccgccg gcctggagga tgcagcgcaa ggagacatcc
     2881 tcttcgttga aaaccggcgc tatgcggagc gcgcggcggc ctcgcaggcc agcgcggtcc
     2941 tcgtgccacc ggatgtggtg atccccaaca agccgtgcat caccgtagaa gatccgcgcg
     3001 cggcgtttct gaaggtgctg tcgttcttcc aaccgcgcga gcgcctgccc cagggaatcg
     3061 cggagaccgc gcggatcggg cagaacgtca ccctgggcga aggcgtggcc atcggcgatt
     3121 actgttgcat cggcgataac gtggtccttg gcgacggcgt ggtgctcttc ccgatggtct
     3181 acgtcggcga taacgtgacc attggtccca acaccgtgat ctacccgcag gtcacggtct
     3241 atcgcgatgt gacgatcgga gcacgggtgc ggattcactc gggcagtgtg atcggcgcgg
     3301 acggattcgg ctacatcttc gtcgaaggcc agcatgtaaa ggtgcctcat atcggcacag
     3361 tgacgatcga agacgacgtg gag
//
LOCUS       DAOSCV010000459         3380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_17931_length_3380_cov_3.568722, whole genome shotgun sequence.
ACCESSION   DAOSCV010000459 DAOSCV010000000
VERSION     DAOSCV010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17931_length_3380_cov_3.568722"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2216
                     /locus_tag="PLY56_11505"
     CDS             <1..2216
                     /locus_tag="PLY56_11505"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOJ22151.1"
                     /translation="LYGSLAPLVEELVDVVFYGRNELETGHVWRHYWHNMDWCYLDLM
                     TYMGDIPRFADAKFAEKAARYNETKSKEWLVREPSLSDKEWLAKVAEHLKGRATQAMR
                     FGGAYDYCMGDEMSLTHYTRYFDYDWSPHALNAFREWLKARYPSLDALNKAWETNFTS
                     WNAVVPMTLHEVTGRANAAPWAEFRNFMNDSVADFFAMAQASLKSVDPQARAGLSGTQ
                     EPRSGNGMDWWKNSKAFNYYHSYNTGWSNEMRRSFAPYTGTAQSPYYAGYWQAGRAVE
                     YNFFWCLLHDTEAVSAWTTPIFFYNDFTYSESGRDTLALCREMKHGLWDLIRNARREH
                     DGIALHYSHDSINAATLLGKDEEIVRHRDAWVKLIEEMGLQYDFVATPQIEEGILRRG
                     DSRGNRYRVLILPESFAISEKEKAEIEAFVRAGGTVIADRGAGLMDGKCRRGERGVLD
                     DLFGIRRTDSGGEGALGIQVRIPGIPASEVKFPVAESLAPTTAKALGQSAGEQPLPAL
                     LRNGVGSGAAWYLNLDLSPFDNERVFHTPAEKGLRAILGAILAEAGVKPKVTVSLASG
                     HAPHLEVVRYTAGDLTYVGLLHAREEKEELATITFPETAHVYDCRKGEALGKLREVRA
                     SFAGGEARVYCLAPKPLAEPRLALATPAAARGEAVRYSVSLNGGSDRQKQLVRLTVYR
                     PDGSEYADYARNLYLGEQGFTGEFRLALNDPTGAWKIVARDVCSGAQAVTTFTVK"
     gene            2365..3012
                     /locus_tag="PLY56_11510"
     CDS             2365..3012
                     /locus_tag="PLY56_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ22152.1"
                     /translation="MSEDRPIQFTDNYEDLSSEKGFQFRFNCERCGNGFMSSFIPNRV
                     GQAGGLLEAAGSMLGGIFGRAASGARSIEQAVAGKQHDAALRKAVEEIRPEFIQCRRC
                     GDWICRDICYNETAKMCKGCAPIAEEEETAIRAGHVQTQVANDLFLEENKRMSAKGKE
                     VAAKCTQCGQPTLGKKFCPECGTPTASAITNCPECGAKLTPGAKFCGECGAKVTG"
     gene            complement(3078..>3380)
                     /locus_tag="PLY56_11515"
     CDS             complement(3078..>3380)
                     /locus_tag="PLY56_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22153.1"
                     /translation="PALLALGNGRVLCVFRRTDEPGLWAAEVSLAGGEWETERLFPLW
                     GAPSLFTGGHRADMVSQFNVLRFGAPSVVGVGDGGAYVTFWCVEECVANIRWIRLG"
BASE COUNT          639 a         1100 c         1131 g          510 t
ORIGIN      
        1 acctctatgg ctccctggcg cccctggtcg aggagcttgt tgatgtcgtc ttctacggca
       61 ggaacgagct ggagacgggg cacgtgtggc gccactactg gcacaacatg gattggtgct
      121 acctcgacct gatgacctac atgggagaca tcccccggtt cgcggatgcg aagtttgccg
      181 agaaggcagc gcgctacaac gagaccaaat cgaaggagtg gctggtgcgc gagccgagcc
      241 tatccgataa ggagtggctg gccaaagtgg ccgagcacct gaaggggcga gccacccagg
      301 cgatgcgctt tggcggcgcc tatgactact gcatgggtga tgagatgagc ctcacccact
      361 acacgcgcta tttcgattac gactggtcac cccacgcgct caacgcgttt cgcgagtggc
      421 tgaaggcgcg ctacccaagc ctcgacgcgc tgaacaaggc gtgggagacc aacttcacca
      481 gctggaatgc cgtggtgccg atgacactgc acgaggtcac gggccgggcc aacgccgccc
      541 cctgggccga gttccgcaac ttcatgaatg acagcgtcgc cgatttcttc gcgatggctc
      601 aggcaagcct gaagagcgtg gatccccagg cacgcgccgg cctctccggc actcaggagc
      661 cgcgctccgg caacggcatg gattggtgga aaaactcgaa ggcgttcaac tactaccact
      721 cctacaacac ggggtggtcg aacgagatgc gccgttcctt cgcgccctat acagggacgg
      781 cccagtcgcc ctactatgcc ggctactggc aggcggggcg cgccgtcgag tacaacttct
      841 tctggtgcct gcttcatgat accgaagcgg tcagcgcctg gacgacgccg atcttcttct
      901 acaacgactt cacctatagc gagtcggggc gggataccct cgcgctctgc cgcgagatga
      961 agcacggcct gtgggacctg atccgcaatg cccggcgcga gcatgacggc atcgccctcc
     1021 actatagcca cgacagcatc aacgccgcca ccctcctcgg caaagatgag gagatcgtcc
     1081 ggcatcgcga cgcctgggtg aagctgatcg aggagatggg cctgcagtac gactttgtcg
     1141 ccacgccgca gatcgaggaa gggatcctgc gccgcggcga ttcccgcggc aaccggtatc
     1201 gcgtcctgat cctccccgag tcgttcgcga tctcggagaa ggagaaggcg gagatcgagg
     1261 cgttcgtgcg cgctggcggc acggtcattg ccgaccgggg cgccgggctg atggacggca
     1321 agtgccgcag aggcgaacgc ggcgttctgg atgatctctt cggcatccgg cgcaccgatt
     1381 ccgggggcga gggcgcactc ggcatccaag tgcgaatacc cggcatcccc gcgtccgagg
     1441 tgaagttccc cgtagccgag tcgctcgcgc ccaccacggc caaggcactc gggcaatccg
     1501 ccggggagca gcctctgccc gccttgttgc ggaacggcgt gggcagcggc gccgcctggt
     1561 acctcaacct ggatctctcg ccctttgaca acgagcgcgt cttccacacg cccgcggaaa
     1621 agggcctccg cgccatcctc ggcgccatcc tggcggaggc gggcgtgaag cccaaggtga
     1681 cggtcagcct cgcctcggga cacgcccccc atctggaagt ggtccgctac accgcgggcg
     1741 atctcaccta cgttggcctg ctgcacgccc gcgaagagaa ggaggaactc gccacgatca
     1801 ccttcccgga gacggcccac gtctatgact gccgcaaagg cgaggcgctg gggaagctcc
     1861 gcgaggtgcg cgcgtcgttc gcgggcgggg aggcccgggt ctactgcctg gcgcccaagc
     1921 cactggcgga gccgcggctg gcgctcgcca cgcctgctgc cgcccgagga gaggcggtgc
     1981 gctactcggt gagcctcaat ggtgggagcg accgccagaa gcagctcgtc cggctaaccg
     2041 tctaccgccc ggatggcagc gagtacgccg attacgcacg caacctgtac ctcggggagc
     2101 agggcttcac gggagagttt cggctggcgc tgaacgaccc cacgggcgcg tggaagatcg
     2161 tcgcgcgcga cgtgtgcagc ggcgcgcagg ctgtcacgac gttcacggtg aagtgagggt
     2221 ttcttctacg gcgagacggg cgcagggaat gcgcacctgc gtggcgaagg gagagacgtt
     2281 gcaggaatca aacagatgcc gcgcaacgcg tgggggccag aagatgccag gccgcgacgc
     2341 ggtctcgtcg cgggaggaag atggatgtca gaagatcgac caattcaatt taccgataac
     2401 tatgaggacc tgagcagcga gaagggtttt cagtttcgct tcaactgcga gcgctgcggg
     2461 aacggcttca tgtcgtcgtt catccccaat cgcgtcggtc aggcgggcgg gctgctcgag
     2521 gcggccggct cgatgctcgg gggcatcttt ggccgggcag ccagcggcgc gcgctctatc
     2581 gagcaagccg tcgcgggcaa gcagcacgac gcggcgctgc gcaaggcggt cgaggagatt
     2641 cgcccggagt tcatccagtg ccggcgctgc ggcgactgga tctgccgcga tatctgctac
     2701 aacgagacgg caaagatgtg caaggggtgc gccccgatcg ccgaggaaga agagacggcg
     2761 atccgcgccg ggcacgtgca gacgcaggta gcgaacgacc tcttcttgga agagaacaag
     2821 cggatgagcg ccaagggaaa ggaagtggcc gcgaagtgca cccagtgcgg ccaaccgacc
     2881 ctcggcaaga agttctgccc ggagtgcggc acgccgacgg cctccgcaat cacgaactgc
     2941 cccgagtgcg gcgcgaagct cactcccggc gcgaagttct gcggcgagtg cggcgcgaaa
     3001 gtaaccggct gatctgacgg ccagccgttt cgaggggggc gtgaccagga cggcgcgccc
     3061 ccctcacttg ctttctccta ccccaggcgg atccagcgaa tgttggcgac gcactcctcg
     3121 acgcaccaga aggtgacgta agcgccgcca tcccccacgc ccaccaccga gggcgcgccg
     3181 aagcggagca cgttgaactg gctcaccatg tccgcgcggt ggcccccggt gaagagcgaa
     3241 ggcgcccccc aaagggggaa gagccgctcg gtctcccact cgccgccggc cagcgagacc
     3301 tccgctgccc acaggcccgg ctcgtccgtc cgccggaaga cgcagaggac gcgcccatta
     3361 cccagcgcga gaagcgccgg
//
LOCUS       DAOSCV010000460         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18009_length_3371_cov_3.808504, whole genome shotgun sequence.
ACCESSION   DAOSCV010000460 DAOSCV010000000
VERSION     DAOSCV010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18009_length_3371_cov_3.808504"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLY56_11520"
     CDS             complement(<1..242)
                     /locus_tag="PLY56_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22154.1"
                     /translation="MISRNWMTTIPPSIALSEAGLPTVPRVHRQALLLLAGLMLLALG
                     ALYVLGGVGRPLATGLGGLIAVGLVLWDLRIGLGLF"
     gene            complement(239..1741)
                     /locus_tag="PLY56_11525"
     CDS             complement(239..1741)
                     /locus_tag="PLY56_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ22155.1"
                     /translation="MRSADFLSLISERRARILRQASLFSLTALAIVVLAGAALAAPET
                     RATGDDYRVQPGDTLEIHLWNEGDKGHDFVVDPLGNISYPMAGILSVKGMTVREITQR
                     LTAELGKHLKAPRVSVNVKALAPQTLLAAGAAKRVYVLGAVAKPGAYELAPGVRVVDA
                     VALAGGLAPNAAASRATLSGEERGKLPLDLEKLLAGGDITHNIPLNPGDVLFVPAQDP
                     ESTSVTVIVIGQVQRPGTHRLPLGARLSDAMSASGGAGERADLKRAKLTRAELTVTID
                     LDAVFRKGDLSGDLALQQGDIILVPERARQEVCLLGEFQKPGSYEFDEGATILDAIAL
                     GGGCTPDADLEGAILKRAGKEIPIDLNAILKQGKLDGLQALENGDVLVVPAGIRVYAL
                     GAVGKPGPLTIRRDAKLIDVLFQAGGPDARARLDRAGVVRIANEQAQVIPLDIKDATQ
                     NGGPDRDFRFAAGDILYIPTKPQAYTWRGILGTATSVASALWLLGAIDRR"
     gene            complement(1741..3003)
                     /locus_tag="PLY56_11530"
     CDS             complement(1741..3003)
                     /locus_tag="PLY56_11530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOJ22156.1"
                     /translation="MEEPNPLYAALVERVREGEIQVEVQRARAEALAKAEAKKAPSTP
                     GKDPKAFMELVALERAKEIAERTYGQILEQLQQVRLNEAQQQGTARRVDIAQVPEQPV
                     HPTPRRTLAIAALLGLVAGLSLALLQELADTGVRDPDQLLMRTGISSLGFIPATRDTA
                     RTAKIAARSPRSPVTESFRTIRSNLKFAHLDSPLHTMMVTSAGVGEGKSFTATNLAIV
                     LAQAGVNTLLVDADLRRPTLHRIFGIPRTPGLTTVLTGETPLREAIQATDVANLYIIP
                     SGPVPPNPAELLESKRMRQLIEEMKEIAEIVIFDTPPALVVTDAVVLSPRMDAMILVI
                     EQGRVTSRAIAEIRRMVEQARGKIAGAVFNKVKSGASGYYYYHYYRYYYYGDHNEGDA
                     QQRRPAMPLTALDHGRNQSQSKGPDEEA"
     gene            3019..>3371
                     /locus_tag="PLY56_11535"
     CDS             3019..>3371
                     /locus_tag="PLY56_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ22157.1"
                     /translation="MRGTLLDQASQSVVAEITAAGGQAAAFDCDITDRASVDAAVAAT
                     QAQLGPVAVLVNNAGWDVFKPFTKTVPAEWDRLIAINLTGALHMHHAVLPGMAERKKG
                     RIVNVASDAARVGSSG"
BASE COUNT          592 a         1081 c         1093 g          605 t
ORIGIN      
        1 atgaagaggc ccaagccaat tcgaagatcc cacaggacga gcccgacggc gatgagtccg
       61 ccgagccccg tggcgagagg ccggccaaca ccccctaaca catagagcgc gccgagcgcc
      121 agcagcatga ggccggcgag caagagcaac gcctggcgat gtacgcgcgg caccgtcggc
      181 aacccggcct cgctcaatgc tatcgatggc gggatggtgg tcatccagtt acgcgaaatc
      241 aacggcggtc gatggcgccc agcagccaga gggcggaggc gaccgaggtc gccgtaccga
      301 ggatgccgcg ccaggtgtag gcctgaggct ttgtcgggat atagagaatg tcgccggcag
      361 caaaacggaa gtcgcggtcc ggaccgccgt tctgcgttgc atccttgata tccaagggga
      421 tgacctgcgc ctgttcgttg gcaatgcgga ccacaccagc gcggtcaagg cgcgcacgcg
      481 cgtcgggccc gcctgcctgg aagagcacgt caatcagctt cgcatcacgg cggatagtca
      541 gcggccccgg cttgcccacc gcgccaagag cgtagacgcg gatgccagcg ggcacaacaa
      601 gaacatcgcc gttctcaagc gcctggagcc catcaagttt gccctgcttc aggatggcgt
      661 tgaggtcgat ggggatctct ttcccagcgc gcttgaggat ggcgccctcc agatctgcgt
      721 caggggtgca tccgccccca agtgcgattg catcgaggat cgtggcgccc tcgtcaaact
      781 cgtaagagcc aggtttctga aactccccaa ggaggcacac ctcctgacgg gccctctccg
      841 gcaccaggat gatatcgccc tgctgcagtg ccaggtcgcc tgacagatca cctttgcgaa
      901 agaccgcgtc caagtcgatg gtgaccgtca attcggcgcg ggtcagcttg gcgcgcttca
      961 gatccgccct ctctcccgcg ccaccgctgg cgctcatagc gtcactcaga cgagcgccta
     1021 gggggagccg gtgcgtgccg ggacgctgga cctgcccgat aacgataacg gtcacgctgg
     1081 ttgattccgg gtcttgggcc ggcacaaaca ggacgtcgcc tgggttcaac gggatgttat
     1141 gggtgatatc gccgccggcg aggagcttct ccagatctag aggtagcttg ccgcgctcct
     1201 ccccggaaag cgtcgcacgc gaggcggccg cgttcggagc gaggccgccg gcgagcgcca
     1261 ccgcgtccac gactcgcacc ccaggggcca gctcatatgc ccctggcttt gcgacggcgc
     1321 cgagcacgta gacacgcttg gccgcgccgg ccgccagcaa ggtctggggc gctagcgcct
     1381 tcacgttaac agaaacgcgg ggcgccttga ggtgcttgcc cagttccgcc gtaagccgct
     1441 gcgtgatttc gcggaccgtc atccccttca cggaaaggat gccggccatc gggtaggaga
     1501 tgtttcccaa gggatcgacg acaaaatcgt gccccttgtc accctcgttc cagaggtgga
     1561 tctcgagcgt atcgcccggc tgaacacggt aatcatcgcc tgtggcccgg gtctctggcg
     1621 ctgccagtgc ggctcccgcc aggactacga tggcgagcgc agtgagcgaa aacaaggatg
     1681 cttggcgcag tatgcgcgca cggcgctccg agatcagcga taggaaatcc gcactcctca
     1741 tcaggcttcc tcatccggtc ctttggattg cgactggttg cggccatgat cgagagctgt
     1801 caagggcatc gctggacgac gctgttgcgc gtcgccctcg ttatggtcgc catagtaata
     1861 gtagcggtag tagtggtagt aatagtagcc actcgcgcca ctcttcacct tattgaagac
     1921 tgcgcccgcg atcttcccac gcgcctgctc gaccatgcgg cggatttcgg cgatcgcgcg
     1981 ggatgtcacc cggccctgct cgatcacgag gatcatggca tccattcgag gcgataggac
     2041 caccgcatcg gtgactacga gtgccggagg cgtgtcgaag atgacgatct ctgcaatctc
     2101 cttcatctcc tcgatcagtt ggcgcatccg cttggactcg aggagttcgg ccgggttggg
     2161 tggcaccggt ccgctcggga tgatatagag gttggcaaca tcggtcgcct ggattgcctc
     2221 gcgcaatggc gtttcgccgg tcaggacggt ggtgagcccc ggtgtgcgcg gaatcccaaa
     2281 aatacgatgc agggtcggcc ggcgcagatc agcgtcgacg agaagggtat tcactccggc
     2341 ctgggcgaga acgatggcga gattggtggc ggtgaagctc ttgccctcgc cgacgccggc
     2401 actcgtgacc atcatcgtat ggagggggct atcgagatgc gcgaacttga ggttcgagcg
     2461 aatcgttcgg aagctctcgg tgaccggcga gcgtggagat cgggcggcga tctttgcggt
     2521 tcgcgctgtg tcgcgcgtcg ccgggatgaa accgagagag gagattcccg tgcgcatcag
     2581 gagttgatcg gggtcgcgca cgccggtatc ggccagctct tgcagcagtg cgagcgagag
     2641 gccggccacc aggccgagaa gtgcggcaat ggccagtgtc cggcgcgggg tcgggtggac
     2701 cggctgctca ggcacttgtg cgatgtctac tctgcgggcc gtgccttgtt gctgcgcctc
     2761 attcaggcgc acctgctgga gctgttccag aatctgtcca taggtgcgct cagcaatttc
     2821 cttggcgcgt tccagggcca ccaactccat aaatgccttg ggatccttac ctggcgttga
     2881 cggggccttc tttgcctccg ccttggcaag cgcctcggcc cgggcgcgct gcacctctac
     2941 ctggatctct ccctcgcgta cccgctccac caacgcggca tagagcgggt tgggctcctc
     3001 cacctcggcg acctttgcct gcgcggcacg ctcctggacc aggcctcgca gtcggtggtg
     3061 gccgagatca cggccgccgg cggccaggcc gccgccttcg attgcgacat caccgaccgc
     3121 gcctcggtgg atgcggccgt cgccgccaca caggcgcagc tcggtcccgt tgccgtgctg
     3181 gtcaacaacg ccggctggga cgtgttcaaa cccttcacca agactgtgcc cgctgagtgg
     3241 gaccggctca tcgccatcaa cctgaccggc gccctgcaca tgcaccacgc cgttctgccc
     3301 ggcatggccg agcgcaagaa aggccgcatc gtcaacgtgg cgtcggacgc cgcccgcgtg
     3361 ggttcctcgg g
//
LOCUS       DAOSCV010000461         3369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18022_length_3369_cov_2.979179, whole genome shotgun sequence.
ACCESSION   DAOSCV010000461 DAOSCV010000000
VERSION     DAOSCV010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18022_length_3369_cov_2.979179"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="PLY56_11540"
     CDS             <1..1475
                     /locus_tag="PLY56_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008028 - monocarboxylic acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015718 - monocarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HOJ22158.1"
                     /translation="GLFPIGWIVVAAVFLYQITVHTGLFAVIRDSIASVTDDRRLQAL
                     IIAFSFGAFLEGAAGFGTPVAISGAMLVGLGFRPLYAASICLIANTVPVAFAALGIPV
                     LAAAEVTGLSPEAIARVIANQLLPLSLIVPAWLVFVMAGWRGVSEVAPAVILSGATFA
                     AIQWWTARQLGPYLPAILASLGSLSALVLLLRVWRPRAAWRFPEEPPATYHAGRWSRV
                     QLVRAWTPFLLLTLLIALWSVRPVARLLESVSVRIPFPGLHEAFIAAGAEAPATVIFK
                     LNWLSAAGTPIFLAAVITLALQRVPPAESARLFAATLRTLAFPLITIMAVLAFAFIAN
                     YSGMSTAMAMALTETGSLYPFLSPVLGWIGVFITGSDTSANALFGNLQASAAREIGMD
                     PVLAVAANTTGGVTGKMISPQSIAVATAAVGLAGRESEILRRTVWHSLAFLLAISVLT
                     YLQASLLPGLAALVPLFPAAWPLGAGGWLVLAGFGTWRRK"
     gene            1472..1759
                     /locus_tag="PLY56_11545"
     CDS             1472..1759
                     /locus_tag="PLY56_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOJ22159.1"
                     /translation="MKRAIRIDPHDNVATAVEAVAPDEEVRISGSGEEITLAAREAVP
                     RGHKVALAALRAGDHVVKYGEVIGRMTAEARPGDCVHIHNMEAIRGRRRSA"
     gene            1756..2925
                     /locus_tag="PLY56_11550"
     CDS             1756..2925
                     /locus_tag="PLY56_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018211354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HOJ22160.1"
                     /translation="MTFQGYRRPDGQAGIRNYILVMPTVICANQVATGIAEGLPAVAI
                     PHVYGCSFDGRDNAALEYTLIGVGRNPNVAAVLVVTLGCETASGEKVVAGIAESGKPV
                     ARVDIQEEGGTLRAIARGRELVEGFAREIEGLRPKPVPVEELILAVECGASDAFSGLS
                     ANPAVGAAADLLVEAGGTVILSETSEIIGAEQVLARRCVDAETRERLLNIVEQQESAF
                     RSFEEHAGGVYIAPGNIAGGLTTLEEKSLGCIRKGGTTPVREVVAFGERPSRRGLVVM
                     DTPGHDVASMIGMVAGGAQVICFTTGRGTPTGCPVAPVIKVCSNTETSRHMADNIDLD
                     AGTILSGEESIEDVGRRIFERVLAVANGERTCSELLGHAEFMIGRRREIGICPRG"
     gene            complement(3061..>3369)
                     /locus_tag="PLY56_11555"
     CDS             complement(3061..>3369)
                     /locus_tag="PLY56_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22161.1"
                     /translation="TDHDLPTHYSFQGEYGVRRFIARYEEVKKKGREAFLRPKESTPE
                     HRAARAKALAPQVEAVIAAQDPQGRWVRNGRIECATFIRNLNTLSEYLELTRNAPAGK
                     "
BASE COUNT          466 a         1127 c         1186 g          590 t
ORIGIN      
        1 atggcctctt ccccatcggg tggattgtgg tcgcggccgt cttcctctac cagatcaccg
       61 tgcatacggg gctgttcgcg gtgatccgcg attccatcgc ctcggtgacc gatgaccgcc
      121 ggctccaggc gctgatcatc gccttctcct tcggggcgtt tctggagggc gcggcggggt
      181 ttggcactcc cgtggcgatc tcgggcgcca tgctggtggg cctcggcttc cggccgctct
      241 atgcggcgag catctgcctg atcgcgaaca ccgtgccggt ggcgttcgcc gcactcggca
      301 tcccggtcct cgccgccgcg gaggtgaccg gcctcagccc agaggcgatc gcccgggtga
      361 tcgccaacca gctcctgccc ctctctctca tcgtacccgc ctggctcgtc ttcgtgatgg
      421 caggctggcg gggcgtgagc gaggtggccc ccgccgtgat cctcagcggc gccaccttcg
      481 cggcgatcca gtggtggacc gcgcgccagc tcgggccgta cctaccggcg atcctcgcct
      541 cgctggggtc gctctccgca ctggtgctcc tgctgcgcgt ctggcgcccg cgcgctgcgt
      601 ggcgctttcc cgaggagccg cccgcgacct accacgccgg ccggtggagc cgggtccagc
      661 tcgtgcgcgc gtggacgccg ttcctcctgc tcacgcttct gatcgccctc tggagcgtcc
      721 ggccggtggc gcgcctgctg gagagcgtca gcgtgcggat ccccttcccg gggctgcacg
      781 aggcgttcat tgccgcaggg gcggaggcac cggctacggt catcttcaag ctgaactggc
      841 tttcggcagc cggaacgccc atcttcctgg ccgccgtgat caccttggcg ttgcagcgcg
      901 ttcccccggc tgagagtgcg cgcctctttg ccgctaccct gcgcacgctc gccttcccgc
      961 tgatcacgat catggccgtg ctcgcgttcg cgttcatcgc gaactactcc ggcatgagca
     1021 ccgccatggc gatggcgctg acggagacgg ggagcctcta tcccttcctc tcgccggtgc
     1081 ttgggtggat cggtgtcttc attaccggga gcgacacctc ggccaacgcg ctgtttggca
     1141 acttgcaggc atccgccgcc agggagatcg gcatggatcc cgttctggcg gtggccgcca
     1201 atacgaccgg cggggtgacc ggcaagatga tctcgcccca atcgatcgcc gtggccaccg
     1261 ctgcggtagg ccttgccgga cgcgagagcg agatcctgcg ccggactgtc tggcacagcc
     1321 tggctttcct gctggcgatc agcgtgctga cctacctgca ggcgagcctc ctgccgggcc
     1381 tggccgcgct ggtgcctctt ttccccgcgg cctggcctct cggagcgggc ggctggctcg
     1441 tcctggcagg cttcggaacc tggagaagga agtgaaacga gctatccgaa tcgatcctca
     1501 cgacaatgtg gcgaccgccg tggaagccgt cgccccggac gaggaggtcc gcatctctgg
     1561 ctcgggcgag gagatcacgc tcgcggcaag agaggcggtt ccccgagggc acaaggtggc
     1621 gctcgcggcg cttcgcgctg gcgaccacgt cgtgaagtat ggcgaggtga tcgggcggat
     1681 gaccgctgag gcgcgtccgg gcgattgcgt ccacatccac aacatggagg cgatccgcgg
     1741 gcgaaggagg agcgcgtgac gtttcaaggc taccgccgtc cagacgggca ggcgggcatt
     1801 cgtaactata tcctcgtgat gccgaccgtg atctgcgcca accaggtggc aaccgggatc
     1861 gcggaggggc tgccggccgt cgccattccc cacgtctatg ggtgcagctt cgatggccgc
     1921 gacaacgccg ccctggagta tacgctgatt ggcgtggggc gcaacccgaa tgtggccgcc
     1981 gtcctcgtgg tgacgcttgg gtgcgagacc gcctccgggg agaaggtggt ggccggcatc
     2041 gccgagagcg gcaagccggt cgccagagtg gatatccagg aagagggcgg cacgctgcgc
     2101 gcgattgccc gcgggcgcga gctcgtggag ggcttcgccc gggagatcga gggcctgcga
     2161 ccaaagccgg tgccggtcga agagttgatt ctggcggtgg agtgcggagc ttcggatgcc
     2221 ttctcggggc tttcagcgaa tcccgccgtt ggcgccgctg ccgacctcct ggtcgaggcc
     2281 ggaggcacgg tgatcctctc ggagacctca gagatcatcg gcgccgagca ggtcctggcc
     2341 cggcgctgcg tggacgctga aacgcgcgag cgcctcctca acatcgtgga gcagcaggag
     2401 agcgcgttcc gctcattcga ggagcacgcg gggggcgttt acatcgcgcc tgggaatatc
     2461 gccggcgggc tcacgacgct ggaagagaag tcgctcgggt gcatccgcaa gggggggacc
     2521 accccggtgc gagaggtcgt cgccttcggt gagcgtccga gccgccgggg gctcgtcgtc
     2581 atggatacgc ccgggcacga tgtggcgtcg atgatcggca tggtggctgg cggggcccag
     2641 gtgatctgct tcaccaccgg gcgcggcacg ccaaccggct gtcccgtggc ccccgtgatc
     2701 aaggtctgca gcaacaccga gacctcgcgg cacatggccg ataacatcga cctggacgcc
     2761 ggcacgatcc tcagtggcga ggagagcatc gaggatgtgg gccggcggat cttcgagcgc
     2821 gtcctggcgg tggccaacgg cgagcgcacg tgctcggagc tgctcgggca cgcggagttc
     2881 atgatcggcc gcaggcgcga gatcgggatc tgcccgcggg ggtaaccctc gagtcctccg
     2941 agagagcatc ctcggtggac tcgcgccggc gtcacggcgc ggggcaaccg cctccagccc
     3001 tccccgcgtg cggagagggc cggaggcgct tcaggtgtct gaagatcgtg actcccgggc
     3061 tcacttgcct gccggggcgt tccgcgtgag ctcgagatac tcgctcaggg tgttcaggtt
     3121 gcggatgaag gtggcgcact cgatccggcc gttccgcacc cagcgcccct ggggatcctg
     3181 agcggcgatc accgcctcca cctggggagc gagggccttc gcgcgcgccg cgcgatgctc
     3241 cggcgtgctc tctttcgggc gcaggaacgc ttcgcgcccc tttttcttca cctcttcata
     3301 ccgggcgatg aaccggcgca cgccatactc tccctggaag gagtagtgcg tcgggaggtc
     3361 gtggtcggt
//
LOCUS       DAOSCV010000462         3367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18037_length_3367_cov_4.186292, whole genome shotgun sequence.
ACCESSION   DAOSCV010000462 DAOSCV010000000
VERSION     DAOSCV010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18037_length_3367_cov_4.186292"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLY56_11560"
     CDS             complement(<1..472)
                     /locus_tag="PLY56_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22162.1"
                     /translation="MVRRTPRTLAAVILAALVSIATWWPGRNAAAAEAKPGKPAASQP
                     ALSIPGDARAGAALMARGDAAAEAGQLQEALEAWMRGYERLFPTFRDRPFKAPVQAEY
                     LAQTTLRERLQEILAKELPDERILAEQLAMAAFGFIAPDFDLKESLLKLHTEQVA"
     gene            complement(511..1590)
                     /locus_tag="PLY56_11565"
     CDS             complement(511..1590)
                     /locus_tag="PLY56_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOJ22163.1"
                     /translation="MSRETGNASKIRVQFASDNYSGICPEAWSMMQEANAGHVLSYGD
                     DEWTERACNLLREIFETDCEVFFALTGTAANALALASLCQSYHSVLCHRAAHVETDEC
                     GAPEFFSNGTKILLVPGENGKIDLDAVEKMVKVRTDIHYPKPRVLSLTQATELGTVYS
                     VEELRAVGELAHRLGLRVHMDGARFANALASLKVTPKEISWQAGVDVLCLGGAKNGLP
                     IGEAVLFFNRELAHEFDYRCKQAGQLLSKMRFVSAPWVGLLHDGAWLRNAAHANACAE
                     KLEAELRQIPGVRVMFPRQANSVFLQLPPAAAAALRARGWRFYTFIGEGGARFMCSWA
                     TTDADIAALVADMRAVLAEEAVHSG"
     gene            1691..2221
                     /locus_tag="PLY56_11570"
     CDS             1691..2221
                     /locus_tag="PLY56_11570"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22164.1"
                     /translation="MLLELDARMRSGQALAPVPARPDAKDATGRGAKNGERMPDNVNG
                     GTRVDLVYEINAPVHPEDVNALNAAVGFRKRAPERVAGALAGCTAYVTVRDGGRLVGI
                     GRLLSDGYLFGYINNMAVHPDYQGRGIGRRILEMLMEQARDLEQVFLYTDTADEFYLR
                     YGFERSEKRLYVYRRH"
     gene            2299..3171
                     /locus_tag="PLY56_11575"
     CDS             2299..3171
                     /locus_tag="PLY56_11575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ22165.1"
                     /translation="MKIMLTGGSGHVGRRVLTRLVGPNEVVSIDRVPHPLGWSGRQEV
                     LDLTQPSLLDRIQETFDAIVHLAAIPDHCQAPWDEVLQNNILSTYNVLRYAVDLGIPK
                     VIFASSDSASGWGLHAKWHRPDYLPIDEKHPSLPSEVHGYAKAFGEQLCQGFSRAYGL
                     RTICLRCSFITAPACYERFLKRLSSPEPREAPGATYAWVDVEDVASAIIRALEYDPGV
                     GKSETFYITAAEHYGTAPTLDLIRRNWGEGIPVDRAYYEENARAPFFDIRKAKSLMGW
                     EPEWSVERMIRAHG"
     gene            3178..>3367
                     /locus_tag="PLY56_11580"
     CDS             3178..>3367
                     /locus_tag="PLY56_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22166.1"
                     /translation="MRRVRIAVALLLLSCAVAGAAEEPVALPVVNPGFERGFEAWANA
                     AAGRFGAFVSLDRQVKRTG"
BASE COUNT          584 a         1066 c         1115 g          602 t
ORIGIN      
        1 cggccacctg ctcggtgtgc agcttgagaa ggctctcctt gagatcgaaa tccggggcga
       61 tgaagccgaa ggcggccatg gcgagctgtt ccgcgaggat ccgctcgtct ggaagctctt
      121 tcgccaggat ctcttgcagg cgctcgcgaa gggtggtttg cgccaggtac tccgcctgga
      181 ccggggcctt gaatgggcgg tcgcgaaacg ttgggaaaag ccgctcatag ccacgcatcc
      241 acgcttcgag ggcttcctgg agctggccgg cctcggcagc cgcgtctccg cgggccatga
      301 gcgccgcgcc tgccctggca tcgcccggaa tgctgagggc gggctgagag gctgcaggct
      361 ttccgggctt cgcctcggcg gcggccgcgt tgcgcccagg ccaccaggtc gcgatgctca
      421 ccagcgccgc gaggataacc gcggccaacg tccgtggagt gcggcggacc atctcatccc
      481 cctctcaatc catccgggcg gtgttccccc ttatccgcta tgaaccgctt cctcggcgag
      541 taccgcgcgc atgtccgcga cgagcgcggc gatgtcggcg tcggtggtcg cccacgagca
      601 catgaagcgc gcgccaccct cgccaataaa ggtgtagaac cgccagccgc gggcacgcaa
      661 ggcggcggct gccgcgggag ggagttgaag gaagacagag ttggcctggc gcgggaacat
      721 cacgcggacg ccagggatct ggcgcagctc cgcttccagc ttctccgcgc aggcgttggc
      781 gtgagctgca tttcgcaacc aagcgccatc gtgcaagagg cccacccagg gcgcggagac
      841 gaagcgcatc ttcgagagaa gttgacctgc ttgcttgcac cggtaatcga actcgtgggc
      901 cagctcgcgg ttgaagaaga ggacggcttc accgatggga aggccattct tggcgccacc
      961 caggcagagg acatccacgc cggcctgcca ggagatctcc ttgggcgtca ccttcagcga
     1021 cgcgagcgcg tttgcgaaac gcgcgccatc catatgcacg cgcagaccga ggcgatgggc
     1081 cagctcgccg acggcacgga gctcctccac tgaatagacg gtgcctaact ccgtggcctg
     1141 ggtgaggctg aggacgcgcg gcttgggata gtgaatatcg gtgcgcacct tcaccatctt
     1201 ctcgacggca tcgaggtcga tcttcccgtt ctcgccgggc accaggagga tcttcgtgcc
     1261 gttggagaag aactctggcg cgccgcactc atccgtctcc acatgcgcgg cacggtggca
     1321 gagaacgctg tggtaggact ggcagagaga ggccagcgcc agggcattcg ccgcggtgcc
     1381 tgtgagtgcg aagaaaacct cgcagtctgt ttcgaagatc tcgcgtagca gattgcaggc
     1441 gcgctcggtc cactcatcat ccccgtagga gagcacgtgg ccggcattcg cctcctgcat
     1501 catcgaccac gcctccgggc agatgcccga atagttatcg ctggcgaatt gcacgcggat
     1561 cttactggcg ttccctgtct cccgactcac cgtgcgcctc cttcctcatc tcatcttgga
     1621 tctggcatgg ccgtgccgga cgcggcggct gttgcctcgc ctattatatc acaaatggaa
     1681 cgcgcgggcg ttgcttctgg agctggacgc ccggatgagg agcggccagg cccttgcgcc
     1741 ggttccggcg cgcccggacg ccaaggatgc caccggaagg ggcgcgaaaa acggagagag
     1801 gatgccggac aacgtcaatg gaggaacgag agtggacctg gtctatgaaa tcaacgcccc
     1861 cgtgcacccg gaggatgtga acgcgctcaa tgccgctgtc ggtttccgga agcgcgcccc
     1921 tgagagggtg gctggcgcgc tcgccggatg taccgcctat gtcaccgtcc gcgatggcgg
     1981 gcgattggtc ggcattggcc gcctcctttc cgacgggtat ctctttggct acatcaacaa
     2041 catggcggtt caccccgact accaggggcg cgggattggc cggcgcatcc tggagatgct
     2101 gatggagcag gcgcgcgacc tggagcaggt gtttctctat acagacaccg cggatgagtt
     2161 ctaccttcgc tatggctttg aacgctcgga gaagcgcctg tatgtctacc ggcgccactg
     2221 agcgtggcgg cgcgcggctg gcagggtacc cccggctccg tgacgaactc cctggtaggc
     2281 ttctgaaggg agacccccat gaagatcatg cttaccggcg gaagcgggca tgttggccgg
     2341 cgggttctca cccggctcgt gggtccgaac gaggttgtga gcatcgaccg cgtgccgcat
     2401 ccgctggggt ggtccggacg ccaggaggtg ctcgacctga cgcaaccctc tctactcgat
     2461 cgcatccagg agaccttcga cgccattgtg cacctcgcgg ccataccgga tcactgccag
     2521 gcgccctggg atgaggtgct ccaaaacaac atcctgagca cctacaacgt cctccgctac
     2581 gccgtcgatc tcgggattcc gaaggtgatc ttcgcaagca gcgattctgc cagcggttgg
     2641 ggcctccatg cgaagtggca tcgcccggac tacctgccga tcgacgagaa gcacccgtcg
     2701 ctcccctccg aggtgcatgg ctatgccaag gcttttggcg agcagctctg ccaggggttt
     2761 agtcgcgcgt atggcctgcg caccatctgc ctgcgctgct ctttcatcac cgcccccgcg
     2821 tgctatgaac gatttctgaa gaggctctct tctcccgagc cacgcgaggc gcctggcgcg
     2881 acctacgcgt gggtggatgt ggaggatgtg gccagcgcca tcatccgtgc cctcgaatac
     2941 gatccgggcg tgggcaagag cgagacattc tacatcaccg ctgccgagca ctacggcacc
     3001 gcgccgacgc tcgacctgat ccggcgcaac tggggcgagg ggatcccggt ggatcgcgcc
     3061 tactatgagg agaacgcgcg cgctcccttt ttcgacatcc gcaaggcaaa gagcctcatg
     3121 ggctgggagc cagagtggag tgtcgagcgc atgatccggg cgcatggata gggaagggtg
     3181 agaagagtgc gaattgcagt ggcgcttttg ttgctatcgt gcgccgtggc cggcgccgcg
     3241 gaggagccgg tggcgctgcc ggtggtcaac cccggtttcg agagaggctt cgaggcatgg
     3301 gccaacgccg ccgccgggcg ctttggtgcg ttcgtctcgt tggaccgcca ggtgaagcgc
     3361 actggcg
//
LOCUS       DAOSCV010000463         3366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18052_length_3366_cov_3.212927, whole genome shotgun sequence.
ACCESSION   DAOSCV010000463 DAOSCV010000000
VERSION     DAOSCV010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18052_length_3366_cov_3.212927"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3366
                     /locus_tag="PLY56_11585"
     CDS             <1..>3366
                     /locus_tag="PLY56_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ22167.1"
                     /translation="DPATEAAYRRLTAAIDPAALDRHVRALSAIPSRVAGYPGSRQAA
                     EYVEQQFRKAGLTNVATEQFEVTVPVSDGLRIKDSVLLVDILTRDDASPLVRHILSRL
                     TPETLQKLNECVPPLREFQQTDEVLQRENAAYQEQLEAHPTAPGTLPLVKPNSLVTAE
                     HNHQEAQAALREKLAALEPYLIDELNWLCYNGPLYTPERFAGVTLSAETRQLLANPSK
                     GGGLLTLNRQLLADAFPGAIAVLATWMDVDGTRYRLYPLWPNQVQTSKLPSPEKGGIQ
                     APLIDVGPGKLANFNGKTVDGSVVMVDFNSAQEWLNAPRLGAKAVLFVEPESTQRGEA
                     EGKFISIPLAIPRFWISREDADAIRSRYQLASDGNYLVPKVRLISEVFWQKRPARNVV
                     GWVEGTDPKFKDQVIVIESYYDSMSVVPDLAPGAESACGVATQIELARLYAGEKPRRS
                     ILFLSTGAHHLALKGIRAYIEQHFPEHEVPPVKTRFVTWLSNQATPVGKGLVTIILGL
                     IAFSLLRTVGRARVFGKIAGGVAGVALIFCIVGFWTLSPQEEQPRKRWYIFTALDLSS
                     KTPRVGMFYKGMYYDYRQDIQRKFADFARVQRENAERIVNTALGGDPKELFADGVNPI
                     SGKDWRNFIPGKVALDHEPVTMAGAMGVGFVTTDDARPYVDTPFDTPEKVSIYNLSRQ
                     ARLLAALYYNMVNDNNDPQSQEELALPVTDPSTFSRMTLTGGFAKLVGRVVEFDPRRS
                     FIPDKEIPGGLAVVENNNKTYMGVRGPMVEQVDDKGGFEFAGVAPLTAYGYNRPTHLA
                     AYHINAETGAIDYAPDLGVSGARDYPLDQYITTGYKEATIVAFKCVSTSLYDLVDPQM
                     LRALTNLTVLDGQSNAEPRMYGYAIARPEAWISHVDDAAVIYSTPPNPDMGEQGTRIK
                     VLMGAGPISTRFVLINSTPQNPEGEGYETTASGAIFNTAYKVAQDMWNMDEYRIQRLA
                     KYRIINQGLNTLHAHAKAELELADEAYAERNYERFDAHSRAAWGYESRAYPDVQKTAQ
                     DVVKGVLFYLWLLLPFSFFMERLLWGFSDLRRQLLAGLAVFVVIFVVLLNVHPAFEIT
                     MNPLIVLLAFIMLALAFLIIVLVIGKFEQQLKE"
BASE COUNT          612 a         1163 c          966 g          625 t
ORIGIN      
        1 tggatccagc caccgaggcc gcctaccgga ggctgaccgc cgcgattgac cccgcggcgc
       61 tcgaccggca tgtgcgtgcg ctctcggcga tcccttctcg cgtggcaggc tatccgggtt
      121 cccgacaggc ggccgagtat gtggaacagc aattccgcaa ggccggactg acaaacgtgg
      181 ccaccgagca gtttgaggtc acagtccctg tgagcgacgg cctgcggatc aaggactccg
      241 tccttctggt ggatatcctc actcgcgacg atgcctctcc cctcgtccgg cacattctca
      301 gccgtctcac tcccgagacg ctgcaaaagc tgaacgagtg tgttcctccg ctgcgcgagt
      361 tccagcagac ggatgaggtg cttcagcgcg agaacgcggc gtaccaggag cagttggagg
      421 cacaccccac cgccccaggc actctcccgc tggtgaagcc caactcgctc gtcaccgccg
      481 agcacaacca tcaggaggca caggcggcgt tgcgcgagaa gctcgccgcg ctggagccct
      541 acctcatcga cgagctcaac tggctttgct ataacggccc gctctacaca ccagagcgct
      601 tcgctggcgt caccctcagc gccgagactc gccagctgct ggccaacccc tccaagggag
      661 gcggtctcct caccctgaac cggcagcttc tcgccgatgc gttccctggc gccatcgccg
      721 tccttgccac ctggatggac gtcgacggca cccgttaccg cctctaccct ctctggccga
      781 accaggttca gacttctaaa ctgccctcgc cagagaaggg cggcatccag gcaccgctga
      841 tcgacgtcgg cccaggcaag ctggccaact tcaacggcaa gactgttgac ggctccgtcg
      901 tcatggtcga cttcaactcc gctcaggagt ggctgaacgc gccgcgtctc ggtgccaagg
      961 cggtcctctt cgtggagccg gagagcaccc agcgcggcga ggcagaaggt aagtttatct
     1021 ctattcctct cgccatcccg cggttctgga tctcgcgcga ggatgcggat gccatccgct
     1081 cccgctacca gcttgcctcg gatggcaact acctggtccc caaggttcgc ctcatctccg
     1141 aggtgttctg gcagaagcgg ccggcacgca acgtggtagg ctgggtcgaa ggcaccgatc
     1201 ccaagttcaa ggatcaggtg atcgtgatcg agtcgtacta cgattcgatg tccgtcgttc
     1261 ccgacctggc gcccggcgcg gagtctgcct gcggcgttgc cacccagatt gagctcgcgc
     1321 gcctctacgc cggcgagaaa ccccggcgca gcatcctctt cctctccacc ggcgcgcatc
     1381 atcttgcgct caagggaatc cgcgcctata tcgagcagca cttccccgag cacgaagtgc
     1441 cacccgtcaa gacccgcttt gtcacgtggc tcagcaatca ggcgacgccc gttggcaagg
     1501 gcctggtgac gattattctc ggtctcatcg ccttcagtct tctgcgcacc gtcggtcgcg
     1561 cccgcgtctt cggcaagatt gcgggtggcg tcgctggcgt ggcgctcatc ttctgcattg
     1621 tcggtttctg gaccctcagc ccacaggaag agcaaccgcg caagcgctgg tatatcttca
     1681 ccgcgctgga cctgagcagc aagacacccc gcgtaggcat gttctacaag ggcatgtact
     1741 acgattaccg ccaggacatc cagcgcaagt ttgccgactt cgctcgcgtc cagcgcgaga
     1801 acgccgagcg gatcgtgaac accgcgctcg gcggcgaccc gaaggagctc ttcgcggacg
     1861 gcgtcaaccc gatctcgggt aaggattggc gcaacttcat ccctggcaag gtggccctgg
     1921 accacgagcc ggtgacgatg gctggcgcga tgggcgtcgg attcgtcacc acggatgatg
     1981 cgcgccccta tgtcgatacg ccgttcgaca ccccggaaaa ggtgagcatc tacaacctgt
     2041 cgcggcaggc acgcctcctg gcggcgctct actacaacat ggtcaacgat aacaacgacc
     2101 cgcagtctca ggaagagctc gcccttccgg tcactgaccc gtcgaccttc tctcgcatga
     2161 cgctcaccgg cggtttcgcc aagctggtgg gtcgcgtcgt cgagttcgac ccgcgccgct
     2221 cgttcattcc ggacaaggag attcccggcg gcctggccgt cgttgagaac aacaacaaga
     2281 cctacatggg cgttcgcggg ccgatggtcg agcaggtgga tgacaagggc ggctttgaat
     2341 tcgccggcgt cgctccactc accgcctacg gctacaaccg ccccacgcac ctcgcggcgt
     2401 atcacatcaa cgccgagact ggggctatcg attacgcgcc cgaccttggc gtctccggtg
     2461 cgcgtgacta tccgttggac cagtacatca ccactggtta caaagaggcg accatcgtgg
     2521 cgttcaagtg cgtgagcacc tcgctctacg atctggtcga cccgcagatg ctgcgcgccc
     2581 tcacgaacct cacggttctc gatggccaga gcaatgccga gccgcggatg tacggctatg
     2641 ccattgcccg ccccgaagcg tggatcagcc acgtggacga cgctgcggtc atctactcga
     2701 ccccgcccaa tcccgatatg ggcgagcagg gcacgcgcat caaggtgctg atgggcgccg
     2761 gtcccatctc cacgcgcttc gtcctgatca actccacccc gcaaaacccc gagggtgagg
     2821 gctacgagac gacggcaagt ggcgccatct tcaacaccgc ctataaggtg gcccaggata
     2881 tgtggaacat ggacgagtat cgcatccagc gtctcgccaa gtaccgcatc atcaaccagg
     2941 gcctcaacac cctgcatgcc catgcgaaag ccgagctgga gctcgccgac gaggcctatg
     3001 cggagcgcaa ctacgagcgg ttcgacgccc actcccgcgc cgcatgggga tacgagtcgc
     3061 gcgcttatcc cgacgtccag aagacggccc aagacgtggt caagggcgtc ctcttctacc
     3121 tgtggctcct gctgcccttc tccttcttca tggagaggct gctctggggc ttctctgacc
     3181 tgcgccgcca gctcctcgcc ggcttggctg tcttcgtcgt catcttcgtc gtcctcctga
     3241 acgtccaccc ggcgttcgag attacgatga acccgctcat cgttctcctc gccttcatca
     3301 tgctggcgct cgccttcctg atcatcgtcc tggtgatcgg aaagttcgaa cagcagctca
     3361 aggagt
//
LOCUS       DAOSCV010000464         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18087_length_3362_cov_3.257938, whole genome shotgun sequence.
ACCESSION   DAOSCV010000464 DAOSCV010000000
VERSION     DAOSCV010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18087_length_3362_cov_3.257938"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="PLY56_11590"
     CDS             <1..1571
                     /locus_tag="PLY56_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ22168.1"
                     /translation="EVYRGGLRVETTLNLQMQEAAEKALIREVQRARREGKKVGQGAV
                     VAVDPHTGHIKVMVGSLDYRDPKGGMYNRAAQGRGRQPGSAFKPFVYTAAIDSGYSPN
                     FILQDTPISFPDGDKVWAPRNYDGKFRGPVTIRQAVEQSINVPAIRMLQMVGVDRVIA
                     YAQRMGIRSPLERNLSLAIGTSEVTPLELASAYGCFATGGLHAKPIAIVRVIGRNDRV
                     MEENRPSLTRAIPYETAKVMADILRGVVTRGTGRRAHVIPGACGKTGTSQDLRDAWFV
                     GFTKDLSAAVWVGNDDYTPMGPSTFGGSTCAPVWVGLMQTALPIIARHQRAQPKADIA
                     RREAQERQVTTPEERQRIRQEIEQNTVFRVVCLESEQLAVRQCPRRKRVGFPRGFEPT
                     ETCVLHGTGADDVGVQEAPVASDSERPSGHEPMPLDSGGAASHPASPVRPDQESGPTA
                     HGNRASSGAAGGAASGSPAGASARIEEVTICTSSNKRANEYCPEVEVRKMPESQIPGV
                     CTLHGASAGADGGT"
     gene            1558..>3362
                     /gene="mrdA"
                     /locus_tag="PLY56_11595"
     CDS             1558..>3362
                     /gene="mrdA"
                     /locus_tag="PLY56_11595"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ22169.1"
                     /translation="MAGHSSDPGHYYHAGRETTLAQIRLRSLMVLIAFLFLVLLLRFW
                     YLQIAQGDFYRQEAERNRRADIELPAPRGIIRDRNGVLLAATRPAFSISILPAEVVPN
                     GLDALLERLATLLGRPKAELLADYEKNRIGRFHPVVIARDVPVDIVTAVAEEQPYLPG
                     VLVRSEPVRIYPRGNFATHLLGYVREINAEELARMRDDGYRSGDIIGKTGVEKLADIY
                     LRGTPGRQSIQRDAYGNQLSILGTEPPIAGNTVNLTLDVRVQAAAERKLQELKGKAGA
                     AVAIDPRNGDVLALASSPSFDPTIFTRRLDPKEYHEKIGSNPKYPLQNRAISSAYPPG
                     STFKIVTSTALLQNGVINTRSTAFCPGYMVIGKPKRCWSTHGTVNVYSAIAKSCDVFF
                     YKYALALSPRNPDPIADWAERFGLGEKTGIPLPSEASGNIPRRKKYKHWYPGDTANTS
                     IGQGYILATPLQIARMISVVANGGTLYRPRVIRNVTDLKGRVIKEFPVEGKKLTGADA
                     IRPEVLKIVREGLRQTVAHGTGAVARLEKIAVAGKTGSAEHHRQKAAHAWFCCYAPFE
                     NPTIAIAVLVEEGGHGSTSSGPIARAMLEAYFGLD"
BASE COUNT          639 a         1100 c         1076 g          547 t
ORIGIN      
        1 acgaggtgta tcgcggcggg ctgcgcgttg agacgactct gaaccttcag atgcaggagg
       61 ctgctgagaa ggccctcatc cgggaagtac agcgtgcccg gcgtgagggt aagaaggttg
      121 ggcagggcgc cgtggtcgcg gtcgacccac acaccggcca tatcaaggtg atggtgggca
      181 gtctcgatta ccgagaccca aagggtggca tgtataaccg tgcggcccag gggcgcggtc
      241 ggcagcccgg ctcggcattc aagccgttcg tctacacggc ggccatcgat agcggctaca
      301 gcccgaactt catcctccag gacaccccga tctccttccc ggacggcgat aaggtgtggg
      361 cgccaagaaa ctatgacggc aagttccgcg gcccggtgac gatccgccag gccgtggagc
      421 agtcgatcaa cgtccctgcc attcgcatgc ttcagatggt gggggtcgac agggtgatcg
      481 cctacgccca acggatgggg atccgaagcc cattggagcg gaacctgtcg ctggcgatcg
      541 gcacatcgga ggtaaccccc ttggagctgg catcggccta cgggtgcttc gccaccggcg
      601 ggctacatgc caagccgatc gccatcgtac gtgtgattgg ccgcaatgac cgggtgatgg
      661 aggaaaaccg gcccagcctg acgcgcgcca tcccctacga gaccgcgaag gtgatggccg
      721 atatccttcg tggcgtggtc acccggggca cgggtcggcg cgcgcacgtg atccccggcg
      781 catgcggcaa gactggcacg tcgcaagacc tccgcgatgc ctggttcgtc ggcttcacca
      841 aagatctttc tgccgccgtc tgggtgggca acgatgatta cacgccgatg ggcccctcta
      901 ccttcggcgg cagcacttgc gctccggtat gggttgggct gatgcagaca gcccttccga
      961 ttatcgcgcg ccaccagcga gcccagccca aggcggatat cgcccgccgg gaagcccagg
     1021 agcgccaggt gacgaccccc gaggagcgcc agcgcatccg ccaggagatt gagcagaaca
     1081 cggtcttccg cgtcgtctgc ctcgagagcg agcaacttgc cgtccggcag tgtccccgac
     1141 ggaaacgagt aggcttccca cgcgggttcg agccgacaga gacgtgcgtg cttcatggaa
     1201 cgggggccga cgacgtcggc gtgcaggagg cgccggtcgc ttcggactcg gagcggccat
     1261 ccggacacga gccgatgcct cttgacagtg gcggagcggc ttcgcatcct gcaagcccgg
     1321 tgcggccgga ccaggagagt ggcccgacag cgcacggcaa ccgggcctcc tccggagccg
     1381 ctggcggggc cgcgtccgga tcgcccgccg gcgcgtcagc gcgaatcgag gaggtcacca
     1441 tctgcacatc gtccaataag cgtgccaacg agtattgccc ggaggtagaa gtcaggaaga
     1501 tgcccgagag ccagatcccg ggcgtgtgta cgcttcatgg agcgagtgcc ggagccgatg
     1561 gcgggacata gcagcgatcc gggacattac taccacgccg gacgagagac gacgctggcg
     1621 cagatccgcc tgcgctcgct catggtcctg atcgcgttcc tgttcctcgt attgcttctt
     1681 cgcttctggt acctgcagat cgctcaaggc gacttctacc gccaggaggc tgaacggaac
     1741 cggcgcgccg atattgagct gcccgcgcct cgcggaatca tccgcgaccg caacggcgtg
     1801 ctcctggccg caacacgacc cgcgttctcg atcagtatcc tccctgccga ggtggtgccc
     1861 aacgggctag atgcactgct cgagcgcctg gccactctcc ttggccggcc aaaggcagag
     1921 ctgcttgcgg attacgagaa gaaccgcatc ggccggtttc accccgtggt gattgcgcgc
     1981 gacgtgccgg tcgatatcgt caccgccgtt gccgaggaac agccctacct gcccggagtg
     2041 ctcgtgcgga gcgagccggt gcgcatctac ccgcgaggca actttgccac ccatttgctg
     2101 ggctacgtcc gcgagatcaa cgccgaggag ctggcgcgca tgcgcgacga cggctaccgg
     2161 tccggcgata ttatcggcaa gacgggtgtc gaaaagctgg cagacattta tctccgcggc
     2221 acaccaggcc ggcagagcat ccagcgcgat gcctatggca accagctgag cattttgggg
     2281 actgagccgc ccatcgccgg caacacagtg aacctgacgc tcgatgtccg ggtgcaggcg
     2341 gctgcggaaa ggaagctgca ggagttgaag gggaaggccg gcgcggccgt ggccatcgac
     2401 ccgcgcaacg gcgacgtgct tgccctggca agcagcccat ccttcgatcc gaccatcttc
     2461 acgcgccgcc tggatccgaa ggagtaccac gagaagatcg gcagcaaccc caagtatccc
     2521 ttgcagaacc gtgccatcag cagcgcctac ccgcccggct ccaccttcaa gatcgtgaca
     2581 tccacggcgc tgctgcaaaa tggagtaatc aacacacggt ctaccgcctt ctgtccggga
     2641 tacatggtca tcggaaagcc aaagcggtgt tggagcaccc acggcaccgt caatgtctat
     2701 tccgccattg ccaagtcgtg cgacgtcttt ttctacaagt acgcgctcgc cctctccccg
     2761 cgcaaccccg acccaatcgc cgattgggca gagcgctttg ggctgggcga gaagacgggc
     2821 atccccctcc cctcagaggc ctctggcaac atcccacggc ggaagaagta caagcattgg
     2881 tatcccggcg ataccgccaa cacctccatc gggcagggct acatcctggc gacaccgctc
     2941 cagatcgccc gcatgatctc agtggtggcc aacggcggca ccctctatcg gcctcgcgtc
     3001 attcgaaacg tgacggatct caaggggcgc gttatcaagg agttcccggt cgagggcaag
     3061 aagctcacgg gggccgatgc gatccgcccc gaggtgctca agatcgttcg cgagggcctg
     3121 cggcagacgg tcgcgcatgg caccggcgcg gtggcccggc tggagaagat cgcggtcgcg
     3181 ggcaaaaccg gctcggcaga gcaccacaga cagaaggcgg cgcatgcctg gttctgctgt
     3241 tacgctccct tcgagaaccc caccatcgcc atcgccgtgt tggtagagga gggaggacac
     3301 ggctccacct catccggtcc cattgcccgg gcgatgcttg aggcctactt cgggctcgac
     3361 aa
//
LOCUS       DAOSCV010000465         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18110_length_3358_cov_3.577657, whole genome shotgun sequence.
ACCESSION   DAOSCV010000465 DAOSCV010000000
VERSION     DAOSCV010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18110_length_3358_cov_3.577657"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1298
                     /locus_tag="PLY56_11600"
     CDS             45..1298
                     /locus_tag="PLY56_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22170.1"
                     /translation="MRIVRYKARTKQEAWQRIRAELGPDAVIISTRLVSPWLRWFGKE
                     YVEVVAAAGAPSPRRSAPPADATTAERVIAAAHEAREQERATAAPVGAVAPESSNGRH
                     SATPHASAGLRRRSTAEVTDLQQEIREIRNALGRLMSWTAPKDDAAEAIVTQLTDRGL
                     SEELARDLVGAARGETGEVTWEGVRAAIAERIDVMGVSDLLAAGRPPKEARDSALPGA
                     SGDGPLVVAFVGPTGVGKTTTVAKLAARHAILDRRAVALVTLDTYRVAAVEQIRTYAQ
                     MLDVPLEVVVSLEDVRTALERHANKELIFVDTIGHGPNDEMQLGQLRMLLSAMAPDQV
                     HLVLAAPTDRRTMLNALERFSRLKPTHLLFTKLDEAVVHGAMVETAIRSGRPVSYVSY
                     GQDVPEKIAPAAVDKLAAAICPEED"
     gene            1298..1969
                     /locus_tag="PLY56_11605"
     CDS             1298..1969
                     /locus_tag="PLY56_11605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOJ22171.1"
                     /translation="MVRDATLSRAIAPNRRIVITFGGQDYTTRVEATDPDEFTVAAPL
                     DERGEVPIRAGDRVEVRSDNEAGQIWFEAYVRGRRAEKVPVIALAWPRSYERQQRREY
                     VRVPIRMPVLLIFPAAEGRPEIRCEARTRDLGGGGLQAQWLHPDTISDLMGRRLIIEI
                     ELPDGGEPVRAVGTVVRAREAGTETTPIAELGIEFVQVSERDRNRICRHIYNRQIELR
                     KRGLL"
     gene            1966..2247
                     /locus_tag="PLY56_11610"
     CDS             1966..2247
                     /locus_tag="PLY56_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22172.1"
                     /translation="MNGPTEDREDPISYAWRLLGVVLSVVALLAVSLPSLLNGEDPLV
                     ASAKAAIAFLAVWGLNRGLAAAFDLDCPPEECSEECSEEPSEEGVQEGE"
     gene            2349..3119
                     /locus_tag="PLY56_11615"
     CDS             2349..3119
                     /locus_tag="PLY56_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOJ22173.1"
                     /translation="MRKDYGELWRRFKLHGDAEAREALILAYTPLVKHIVDRMNISPM
                     SYMDHDDLLTHAIIGLIDAIEKCDYERYEQFEAYARTRIRGAVIDGIREMDWVPRVTR
                     RAANRVESVRAQLTASLGRDAMPEEIAEAMNMDTERVQGVLAEVQLGGMLSLDDPMAT
                     GDDDAVTLLDIVPHPGSPNPAASIDALTRKETLARAIDRLGPDERLVITLYYYEDLTL
                     KEIGRIIDRTEARVCQIHKAALRKIHAYLTSEEEIFAA"
     gene            complement(3138..>3358)
                     /locus_tag="PLY56_11620"
     CDS             complement(3138..>3358)
                     /locus_tag="PLY56_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22174.1"
                     /translation="MLKRLYADGLSSEEIRVRRGNVGNAPVGVILARGESIAQVTKPE
                     ADLMKRPPAAAAQNWALNLREALRTLGR"
BASE COUNT          592 a          996 c         1193 g          577 t
ORIGIN      
        1 cccgaggctc aggtgcaatc gcggggcgtg gtaaggattg agcaatgagg atcgttcgct
       61 ataaggcccg tacaaagcaa gaggcctggc agcggatccg ggcagagctg gggcccgatg
      121 ccgtcatcat cagcacgcgc ctggtgtcgc cgtggcttcg gtggtttggc aaggagtatg
      181 tggaggtcgt ggccgcggcc ggtgctccga gcccgcggcg gtctgctcct cccgcggatg
      241 ccaccaccgc cgagagagtg attgcggcag cacatgaggc ccgggagcag gagagggcga
      301 cggcggcgcc ggtcggcgcg gtcgccccgg agagctccaa cggccgccac agcgctacgc
      361 cccatgcaag cgccggtctt cgtcggcgct ccacggcgga ggtgaccgac ctgcagcagg
      421 agatccgcga gatccgcaat gccctcggtc gcttgatgtc ctggacggcc cccaaggatg
      481 acgcggccga agcgatcgtc acgcagctga cggatcgcgg cctttccgaa gagctggccc
      541 gggacctggt gggtgccgct cgaggcgaaa ccggcgaggt cacctgggag ggggtgcgcg
      601 cggccatcgc ggagcggatc gacgtgatgg gagtgtccga cctgctggcg gcgggcaggc
      661 cacccaagga agcgcgagat tccgcccttc ccggtgcctc tggcgatggg ccgttggtag
      721 tggcttttgt tggacccacc ggagtgggga agacaaccac ggtcgccaag ctggccgccc
      781 ggcatgcaat tctggatcgc cgtgcggtgg cactggtgac gctggatacc tatcgtgtgg
      841 cggcggtcga acagatccgc acctacgccc agatgttgga tgtgccgctc gaggtggtgg
      901 tcagcctgga ggatgtgcgc actgcgctcg aacggcacgc caacaaggag ctgatcttcg
      961 tcgatacgat cgggcatggc ccgaacgacg agatgcagct cgggcagttg cggatgctcc
     1021 tttccgcaat ggcgcccgac caggtccatc tggtcctggc ggcacctacc gaccgccgta
     1081 cgatgttgaa cgcactggag cgtttctcgc gcctgaagcc gacacacctc ctcttcacga
     1141 agttggatga ggcggtggtc cacggcgcga tggtggagac ggctatccgt tcaggccggc
     1201 cggtctccta tgtcagttat gggcaggatg tgccggagaa gatcgcaccg gcggccgtcg
     1261 acaagctggc ggctgcgatc tgcccggagg aggattgatg gttcgcgatg caacactgag
     1321 ccgggccatt gcccccaacc gccgcatcgt catcactttc ggcggccagg attataccac
     1381 gcgtgtggag gctacggacc cggatgagtt tactgtggcg gcgcctttgg atgaacgagg
     1441 agaggtgccc atccgcgccg gcgacagagt ggaggtccgc tctgataatg aggccggcca
     1501 gatttggttt gaagcctatg tgcgtggcag gcgcgcggag aaggtgcctg tgatagctct
     1561 tgcctggccg cgcagctatg agcgccagca gcggagggag tatgtgcgcg tgccgattcg
     1621 catgcccgtg ctcctcatct ttccggcggc cgaaggccgg ccggagatac gctgtgaggc
     1681 ccggacgcgc gatctgggtg gtggcgggtt gcaggcgcag tggctccacc cggataccat
     1741 ctccgatctc atggggcggc gcctgatcat agagatcgag cttcctgatg gcggcgagcc
     1801 ggtgcgcgca gtcggcacgg tggtgcgcgc gcgcgaggcg ggcacggaga cgactcccat
     1861 tgcggagctt gggatcgaat tcgtgcaagt gagcgaacgc gatcgaaacc gcatctgccg
     1921 ccacatctac aaccggcaga ttgaactgag gaagagggga ctgctgtgaa cggcccaacg
     1981 gaagatcgcg aggatccgat cagctacgcc tggaggctac tgggcgtcgt cctgagcgtg
     2041 gtggcgttgc tggcagtcag cctgccgtcg cttctgaacg gcgaggatcc tttggtggcg
     2101 agtgccaagg cggcaatcgc ctttctggcg gtgtggggac tcaaccgggg gctcgcggcg
     2161 gcgtttgatc tggactgccc acctgaggag tgctcggaag agtgttcgga ggaacccagc
     2221 gaagagggcg tgcaggaggg cgagtagcat ccgagagggt gcccggagtg aggtctctca
     2281 cctcgggaac gctcgggtga tggctacctg gcggctcttt gggagaccgc gaggctttgg
     2341 agggggagat gcgcaaagac tacggcgagc tatggcgaag gttcaagctc cacggggacg
     2401 ccgaagcccg tgaggcgctc atcctcgcct acacgccgct ggtaaagcac atcgttgacc
     2461 ggatgaacat cagccccatg tcgtacatgg atcatgatga cctcctcacg catgccatca
     2521 tcggcttgat cgacgccatc gagaagtgcg actacgagcg gtatgagcag ttcgaggcgt
     2581 atgcgcgcac gcgcatccgg ggcgccgtga ttgatggaat ccgggagatg gattgggttc
     2641 ctcgcgtcac tcgtcgggcc gccaatcggg tggagagcgt gcgtgcccag ctgaccgcct
     2701 ccctggggcg ggatgcgatg cccgaggaga ttgcggaggc gatgaacatg gatacggagc
     2761 gcgtgcaggg ggtcctggcg gaggtccagc tgggcggtat gctctcgctc gatgatccga
     2821 tggcgaccgg cgatgatgat gccgtgaccc tcctggatat cgtgccccat cccggcagtc
     2881 ccaaccctgc cgccagcatc gatgccctga cgcgcaagga gaccctcgcg cgtgcgatcg
     2941 accggctggg cccggatgag cgcttggtga tcaccctcta ctactatgag gacctgacgc
     3001 tcaaggagat tggccgcatc atcgatcgga cggaggcacg cgtgtgccag atccacaaag
     3061 cagcgctacg gaagatccac gcctatctga ccagcgagga ggagatcttc gctgcgtgag
     3121 cgcgaggcgc ccggccgtca gcgtccgagg gtacggagcg cctcgcgcag gttgagtgcc
     3181 cagttctgtg ccgccgccgc gggggggcgc ttcatgagat ccgcctctgg tttcgtaacc
     3241 tgcgcgatag actcgccgcg cgcgagaatc actcccacgg gcgcgttacc cacgttaccc
     3301 cgccggactc gaatctcctc tgaagaaagc ccgtcggcat agagacgctt gagcatcg
//
LOCUS       DAOSCV010000466         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18113_length_3358_cov_3.256434, whole genome shotgun sequence.
ACCESSION   DAOSCV010000466 DAOSCV010000000
VERSION     DAOSCV010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18113_length_3358_cov_3.256434"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLY56_11625"
     CDS             complement(<1..983)
                     /locus_tag="PLY56_11625"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOJ22175.1"
                     /translation="MPVVNQPSAARLNRGPLLFRYIDDPEQYRIERYLEHGGYTALRK
                     ALREMTPAQVAEMVARANLRGRGGAFFPTARKWSLMPPGVFPRYVVANADESEPGTCK
                     DRQLMERDPHQLIEGLTLAAYSVGAEQVYIYIRWEFEFAAERLWNAIREAYAHGFLGK
                     RILGTEFNCDMAVHRGAGAYICGEETALLSSLEGRRPEPRLRPPYFPAAKGYRSLPTA
                     LSNVETYCNVTHIVREGPEVFTQFGREKCPGTKLTCISGHVRTPGVYEIPLGVPLREV
                     IDEWGGGVREGRSLKAVIPGGASAPILPPDRIDYPFDFEGMIEAGSMIGSAG"
     gene            complement(1095..1589)
                     /gene="nuoE"
                     /locus_tag="PLY56_11630"
     CDS             complement(1095..1589)
                     /gene="nuoE"
                     /locus_tag="PLY56_11630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOJ22176.1"
                     /translation="MSEERRSLAETCAGELQEILSRYLDRRSALLPVLQLAQREYGWI
                     SDDTMREVARIVGCRPVDVYEAVTFYSIFHRQPPGKYVLEVCRTLSCALLGGRKLTQH
                     LEERLGIRCGETTPDGLFTLREVECIGACSNAPAMLINNRIYEDLTPEKLDAILDELR
                     AEAM"
     gene            complement(1586..2788)
                     /gene="nuoD"
                     /locus_tag="PLY56_11635"
     CDS             complement(1586..2788)
                     /gene="nuoD"
                     /locus_tag="PLY56_11635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOJ22177.1"
                     /translation="MGATTDSLTPEIRTETMVLNMGPQHPSTHGVLRLVLELDGETVV
                     RCVPHLGYLHTGFEKTYEDKTYHQCIPITDRMDYLAPLSNNLAFCLAVEKLLGIEAPE
                     RAQYLRVILVELQRICSHLVWLGTHSLDLGAVTPFWYTFRERDQILDLFEELSGVRMM
                     TSYIRIGGLALDLPPGWLEKLREFLAIFPSRVDDYEALLTRNPVFLQRTRGVGELSAK
                     DAIALGASGPVARASGIAHDLRKAEPYCVYDRFEFDIPVGERGDVYDRYRVRVAEMRE
                     SHRIITQALEQLPDGDYKLRDWRYTLPPREAIKRSMEELIFHFKFITEGIRPPVGEVY
                     RPIEGPKGEIGFYLVSDGSPRPLRCRVRPPSFINLQALPKICEGRLVADLVAILGSID
                     IVLGEVDR"
     gene            complement(2902..>3358)
                     /locus_tag="PLY56_11640"
     CDS             complement(2902..>3358)
                     /locus_tag="PLY56_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOJ22178.1"
                     /translation="ESFPEYFRDASTFRGEQTLVIAREGILPVLQLLRDDPQLRFDFL
                     ADLTAVDFLPREPRFDVVYHLKSLSRNVRLRVKAGVPEDDAVIESAVPLWPGAEWPER
                     EVFDLFGISFRNHPDLRRILLPDDWEGHPLRKDYPMGKVAVNFTKRRAR"
BASE COUNT          603 a         1127 c         1067 g          561 t
ORIGIN      
        1 cccgccgagc cgatcatcga accggcctcg atcatcccct cgaagtcgaa gggatagtcg
       61 atccggtccg gtggcaggat aggcgccgag gcgccgccgg ggatgaccgc cttcaggctg
      121 cgcccctcgc gcacgccccc gccccactcg tcgatcacct cgcgcaacgg cacgccgaga
      181 gggatttcgt acacgcccgg ggtccgcacg tgcccgctga tgcacgtgag cttcgtgcca
      241 gggcacttct cccggccaaa ctgggtgaac acctccgggc cctcacggac gatgtgtgtc
      301 acgttgcagt aggtctcgac gttgctcagc gccgtaggca gtgaccgata gcccttggcc
      361 gccgggaagt aaggggggcg cagccggggc tccgggcgcc taccttccag actggagagg
      421 agcgcggtct cctcgccgca aatgtaggcg cccgcgccac ggtgcactgc catgtcgcag
      481 ttgaattccg tgcccaagat cctcttgcca aggaagccat gcgcgtaggc ttcgcggatg
      541 gcgttccaca accgctcggc ggcgaactcg aactcccaac gaatgtagat atacacctgc
      601 tcggcgccga cgctataggc cgccagggtg aggccctcga tcagctggtg cgggtcacgc
      661 tccatcagct gccggtcctt gcacgtgcct ggctcgctct cgtcggcgtt ggcgacaacg
      721 tagcggggga agacgcccgg gggcatcagg ctccacttgc gcgccgtggg gaagaaggcg
      781 ccgccgcgcc cgcgtaggtt ggcccgtgcg accatctcgg ctacctgggc aggcgtcatc
      841 tcgcgcagcg ccttgcgcag cgccgtgtag ccgccatgct ccagatagcg ctcgatccgg
      901 tactgctccg ggtcgtcaat gtagcggaag aggagcggcc cacggttcag ccgggccgcc
      961 gagggctggt tgactaccgg catcgcgcgc gtcctcctcc cggcgtcacc acccgctctc
     1021 gcccggctcc gaacgagggc gagagaggcg ccactctgct cacccggcgt gtcgcgcgtc
     1081 tcgcgtccaa catctcacat cgcctccgct cgaagctcgt ccaggatggc atccagcttt
     1141 tcgggcgtca gatcctcata aatccggttg ttgatgagca tcgccggcgc gttggagcag
     1201 gcgccgatgc actccacctc gcgcagggta aagaggccat ccggggtcgt ctcgccacag
     1261 cggatcccga gccgctcttc cagatgctgg gtgagcttcc ggccacccag caacgcacac
     1321 gaaagggtgc ggcagacttc cagcacgtac ttccccgggg gctggcggtg gaagatcgaa
     1381 tagaaagtga cggcttcata cacatccact ggccggcatc cgacgatgcg cgccacctcg
     1441 cgcatcgtgt cgtcggagat ccagccgtac tcccgctggg ccagctgcag aaccggcagg
     1501 agagccgagc gccggtccag atagcgagag aggatctcct gaagctcgcc ggcgcacgtc
     1561 tccgccaggc tacggcgctc ctcgctcatc gatccacctc cccaagcacg atatcaatgc
     1621 tgccgagtat cgcgaccaga tccgccacga gccggccctc gcagatcttg gggagcgcct
     1681 gcaggttgat gaaggagggc ggacggacgc ggcagcggag agggcgcggg ctcccatcgc
     1741 ttacgaggta gaagccgatc tcccccttgg ggccctcgat gggccgatac acctcgccca
     1801 cgggcgggcg aatcccctcg gtgataaact tgaagtggaa gatcagctct tccatgctgc
     1861 gcttgatcgc ttcgcgaggc ggcagggtgt agcgccaatc gcgcagcttg taatcgccgt
     1921 ctggcagctg ctccagcgcc tgggtgatga tccggtggct ctcgcgcatc tccgcgacgc
     1981 gcacgcgata gcggtcatac acgtcaccgc gctcgcccac cggaatgtcg aactcgaagc
     2041 ggtcgtaaac gcagtagggc tccgccttgc gcaggtcgtg cgcgatcccc gaggcgcgcg
     2101 ctaccggacc gctggcaccc agggcaatcg cgtccttcgc cgagagctca cccacgcccc
     2161 gggtgcgctg gaggaagacc gggttgcgcg tgagcaacgc ctcatagtca tccacgcggg
     2221 agggaaagat cgccaggaat tcgcgcagct tctcgagcca tccaggaggc agatccagcg
     2281 ccaggccgcc gatccggatg tagctggtca tcatgcgcac gccagagagc tcttcgaaaa
     2341 gatcgaggat ctggtcgcgc tcgcggaaag tgtaccagaa gggggtcact gcgcccaggt
     2401 caaggctgtg cgtgccaagc cagaccaggt gactgcagat gcgctgtaac tctaccagga
     2461 tgacgcgcag gtactgggcg cgctctggcg cctcgatgcc caggagcttc tccaccgcca
     2521 ggcagaaggc gaggttgttc gagagcggcg ccaggtagtc catccggtcg gtgatcggga
     2581 tgcactggtg gtaggtctta tcctcgtagg tcttctcgaa gccggtatgc agatacccca
     2641 gatgcggcac gcagcgtacc acggtctcgc catccagctc gaggacgaga cggagcacgc
     2701 cgtgcgtgct cgggtgctgt ggccccatgt tgaggaccat cgtctcggtc ctgatctccg
     2761 gagtcaggct gtctgttgtt gcacccatcc gcatgctccc acggaaaccg gctcctgctt
     2821 catacgaagg cagagagaca tcaggctcgc acttccagat ccatccccgt gctcacaggt
     2881 tccccctcgt gaggccagcc gctatcgcgc gcgtctcttc gtgaaattga ccgccacctt
     2941 ccccatgggg taatccttgc gcaaggggtg accctcccag tcatccggca acaagatgcg
     3001 ccgcaggtcg gggtggttgc ggaaggagat cccgaagaga tcgaacacct ctcgctccgg
     3061 ccattcggcg cccggccata ggggcacggc gctctcgatc accgcgtcat cctctggcac
     3121 gcccgccttc acgcgcaggc gaacgttgcg cgaaagtgat ttcaagtggt aaacgacgtc
     3181 aaagcggggc tctcgcggca ggaagtcgac cgccgtcaga tcagccagga agtcaaagcg
     3241 cagttggggg tcatcgcgaa gcagctgcaa caccggaagg atgccttccc gggcgatcac
     3301 cagggtctgc tccccgcgaa aggtgctggc atcccggaag tactccggaa aactctcg
//
LOCUS       DAOSCV010000467         3356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18138_length_3356_cov_3.297486, whole genome shotgun sequence.
ACCESSION   DAOSCV010000467 DAOSCV010000000
VERSION     DAOSCV010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18138_length_3356_cov_3.297486"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLY56_11645"
     CDS             <1..229
                     /locus_tag="PLY56_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOJ22179.1"
                     /translation="GDGAKTGYELSLIRQMVAAVSAPVVASGGAGELSHFLDAVEAGA
                     TILLAASVFHFGIITIPELKEYLKAHGVKVG"
     gene            649..1812
                     /gene="recF"
                     /locus_tag="PLY56_11650"
     CDS             649..1812
                     /gene="recF"
                     /locus_tag="PLY56_11650"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ22180.1"
                     /translation="MLELMQIEKLLLVDFRSCAHVTLEPGPGINVLVGGNAQGKSTLL
                     EAVYLLATSRSPRAGKDVELIRWGQEFATASVEVLREDRGEVGVEVSLSRTDRRVMRV
                     NGVRRSRVADVVGQLNAVLFTAADLDIIRGEPSLRRRFLNLEISQVSPQYCHALLQYR
                     RILEQRNRLLKSLRDHGGSAETLDAWSEQLVAVGARMMERRLAFTMDLGRHAQRIYRE
                     IAGSGEELNIHYLPSLPIEAGGDIASAFRSRLEEVRQEELARGITLVGPHRDDLGFQV
                     KGVDVRVFGSQGQQRSVALSTKLAEVELMRERVGEAPVLLLDDAGAELDERRRERLFD
                     TVRECCQTLVTCTDEAMLPAQIVRNAQRYRVEEGRVARGDDLMTHDDRVGQHR"
     gene            1812..2408
                     /locus_tag="PLY56_11655"
     CDS             1812..2408
                     /locus_tag="PLY56_11655"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOJ22181.1"
                     /translation="MAPRKNRQLGSVGNALDSAFARLGLSGRLAQQRAVTLWPEVVGP
                     QIAASTRAVAVRDGILFVATKSSTWAHELIFRKKAIMEALERELGRGVLADIRFQARG
                     FSDLEEPEEADRLPAPDRSEWSQIALTEADKAMLEEALEGVSDPDLRARLERVWADSV
                     RIRRWKEAHGWARCARCGLPHRGESELCAICRPRGARA"
     gene            2405..>3356
                     /locus_tag="PLY56_11660"
     CDS             2405..>3356
                     /locus_tag="PLY56_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22182.1"
                     /translation="MTSAIQKRLASRPLVIAGALSFIALLAELGYQTTAVAGMPFFLK
                     DELRLPVRIITLINVCFLVAETGAKIPFGVLSDRYGRRFFIICGPLLSALAAFAITRG
                     PSPNQVAAIRALDGIGAAMLWPALFATVADVVEERIRATAMSLFNAMYIGGIVAGPLL
                     YSGVFSRTGSQVAIFWVLALFFVMAAGVAYFVSPRRATNDRRPAASGEEVPDASAWRV
                     VRGNPVLMAMLVVAVIQFVGLHMLNGVLSIYLNEQIQVETEQIGHLFLYIGLVVGPFA
                     IPMGRLADRIGKPRAVRLGLGVCSVGMWLLPWVRAVPLLVI"
BASE COUNT          561 a         1005 c         1127 g          663 t
ORIGIN      
        1 gggagacggc gccaagacgg gatacgagct gtcgttgatc cggcagatgg tcgccgcagt
       61 ctccgcgccc gttgttgcct ccgggggtgc aggtgagctg agccacttcc ttgacgcggt
      121 cgaggcagga gccaccatct tgcttgccgc ttctgtcttc cacttcggga tcatcaccat
      181 ccctgagctc aaagagtatc tgaaggctca cggggtaaag gttggttaac gcgcacagag
      241 agaggcgcgc gagtatgccg gctgcttgcc ctcctctctc tgcgctgccc ggggataacg
      301 ggttgttcaa taagccgtgg gacccacgct gccatgaagc tcctctccac gcgttttgtg
      361 gagaatgtgg ataaccgccg ctcttttcaa cacctgtccc accagttttc cacatcgctc
      421 tcctcgtccg tccgcctctg taccgggtct ccctcgaccg tccgaaaacc gcgcggttgc
      481 gccgcttcta accactctgg gcttcccgcc acgccaggcc tcagttcact ccggggcgca
      541 aaggaatcac actctcaaca gtctaataag aaccacagct gatcccataa agatagggaa
      601 cttggagaat ccgcgctgtg catttcgaga gaagcgtgtg cggagatatt gcttgagctc
      661 atgcagatag aaaagttgtt gctggttgat ttccgctctt gtgcgcatgt cacccttgag
      721 cccgggccag gcatcaacgt gctagttggc ggcaacgccc agggaaagag cactctcctc
      781 gaagcggtct acctgcttgc gaccagtcgc tcgccacgcg ccggcaaaga tgtggagttg
      841 atccggtggg ggcaggaatt cgcgacggcc tcggtagagg ttttgcgcga ggatcgtggc
      901 gaggtgggtg ttgaagtgag tctgagccgg accgaccggc gcgtgatgcg tgtcaacggc
      961 gttcggcggt cccgagttgc cgatgtggtg ggccaactca acgcggtcct ctttaccgcg
     1021 gccgacctcg atatcatccg aggggaacct tctctgcgcc ggcgcttcct gaacctggag
     1081 atctcccagg tcagtccaca atactgccat gcgctgctcc aatatcggcg catcctggag
     1141 caacgcaatc ggctgttgaa gtcgttgcgc gaccatggcg ggagcgccga gaccctggat
     1201 gcctggagtg agcagctcgt agcggtgggg gcacgcatga tggagcgtcg cctcgctttc
     1261 acgatggatc tggggcggca tgctcaacgc atctatcggg agatcgccgg atccggggaa
     1321 gagctgaata ttcactatct ccccagcctc ccgattgagg caggaggcga catcgcctcc
     1381 gcctttcggt cgcggctgga agaggtgcgg caggaggagc tggcccgggg gatcacgttg
     1441 gttgggccgc atcgggatga cctcggtttc caggtgaaag gcgtcgacgt gcgcgttttt
     1501 gggtcgcagg gccaacagcg cagcgtagct ctctccacca agctggccga ggtggagcta
     1561 atgcgcgagc gggttgggga agccccagtc ctcctattgg atgacgccgg cgcggagctg
     1621 gatgagaggc gcagggagcg cctctttgac acggtgcgag agtgttgtca gacgctggtc
     1681 acctgcaccg acgaggcgat gttgccggcg cagatagtgc gtaacgcgca acggtataga
     1741 gttgaggagg ggagggtcgc ccgcggcgac gatctcatga cacacgatga tagggtgggg
     1801 cagcaccgct gatggcgcct cggaagaatc gacagcttgg ctcggtggga aacgcgcttg
     1861 actccgcgtt cgcgcgcctg gggctttccg ggcggctggc tcaacagcgc gccgtcaccc
     1921 tatggccgga agtcgtcggc ccccagattg ccgcttccac gcgcgccgtg gcggttcgcg
     1981 acgggatcct cttcgtggct actaagagca gcacctgggc ccacgagctg atcttccgca
     2041 agaaggcgat catggaggcg ttggagcgcg agctgggacg aggagtgctc gcggatatcc
     2101 gcttccaggc gcgcggcttc agtgacctgg aagagcccga ggaggcggat cgcctcccgg
     2161 cgccggatcg ctccgagtgg agccagatcg cgctcacgga agccgataag gcgatgctcg
     2221 aagaggctct cgaaggagta tcggatcccg atcttcgagc gcgcctggag agagtgtggg
     2281 ccgactcggt tcgcattcgc cgctggaaag aggcgcacgg ttgggcacgc tgcgcgcggt
     2341 gcgggctgcc gcatcgcggt gaatcggagc tgtgtgccat ctgccgcccg cgcggggcaa
     2401 gagcatgaca tcagcgatcc agaagcggct tgcgtcccgc ccgctcgtca tcgccggcgc
     2461 gctctcattt atcgccctcc tcgccgagct gggctaccag acgaccgcgg tggccgggat
     2521 gccgttcttc ctgaaggacg agctgcgcct tcccgtgcgg atcatcaccc tgatcaacgt
     2581 ctgcttcctg gtggcggaga caggggccaa gatccccttt ggagtgctga gcgaccgcta
     2641 tgggaggcgg ttcttcatca tctgcgggcc acttctttcc gcgcttgccg cgttcgccat
     2701 cacgcgggga ccgtcgccca atcaggtggc ggcgattcgc gccctggatg gcattggcgc
     2761 ggcaatgctg tggccggcgc tcttcgcgac ggttgccgac gtggtggaag agcgaattcg
     2821 cgccaccgcg atgagccttt tcaacgcgat gtacatcggc gggatcgtgg ccggcccgtt
     2881 gctctacagc ggcgttttct cgcgcaccgg ctcgcaggtg gcgatcttct gggtgttggc
     2941 actcttcttc gttatggcgg ccggcgtggc ctatttcgtc tcgccgcgcc gggcaacaaa
     3001 tgaccggcga ccggcggcca gcggcgagga ggtgccggac gcctcggcgt ggagggtcgt
     3061 gcgcggcaat ccggtgctga tggcgatgct ggtcgtcgcg gtgattcagt tcgtcgggct
     3121 gcacatgctg aacggcgtgc tcagtatcta cctcaacgag cagatccaag tcgagacgga
     3181 gcagatcggg cacctcttcc tctacatcgg actggtggta ggaccctttg ccattccgat
     3241 gggccggctc gcggatcgca tcggaaaacc ccgcgcggtg cgcctgggcc tgggggtttg
     3301 ctcggtggga atgtggttgc ttccctgggt gcgggccgtg ccgctcctcg ttatta
//
LOCUS       DAOSCV010000468         3343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18223_length_3343_cov_4.449818, whole genome shotgun sequence.
ACCESSION   DAOSCV010000468 DAOSCV010000000
VERSION     DAOSCV010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18223_length_3343_cov_4.449818"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /locus_tag="PLY56_11665"
     CDS             complement(<1..2144)
                     /locus_tag="PLY56_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ22183.1"
                     /translation="MAQTQNITVSGGILTQQFIEALREERVAHPKAQPDSFTTQDGQK
                     PKPAELAKDITAAWNLALEHWETVRARVRELDLPAARRRWIIPFLHLLEFEPHYLRSA
                     VTVPIEGGEELRFSLSHRGWSGDHAPIIHTVAPAQSLDAREGTGRGAKSPHDTLQAYL
                     NAARDETWGIVTNGISLRLLRDFHHTYTKGYVEFDLQNILEERSFSDFLALYRLCHSS
                     RFLPDAEGKPPLEHFYEHCIATGIAVGRDLQGNVKHAIEALANGFLQGELIGKLQRDD
                     QACRDFYSEILHVIYRILFLLYAEQRGMMPGRNSLYAQEYSITALRERAEKGVPARDT
                     HLDLWEGLKVTFRMVEKGVPELGIFPYNGALFSPEKIGRLNDLTCCNSELLKAIRYLT
                     LIEKGRVLQRISYADLGVEEIGSIYESLLEYTPRVTTTREEIDGQPVPANRFILDPRG
                     SGRKTSGSYYTPPQLVNLLIESALKPVFEERVREALKLPIKQPLTLTSLKPEQQQAAE
                     EAILALRVCDPACGSGAFLTAATNFLGQQLAIVRTGDEYPPEAEVRKARRDVLSHCIY
                     GVDLNPMAVELCKVSLWINACVQDLPLNFLDGHIRCGNSLIGVLDPTVLKKGIPDAAF
                     TPVTGDNTAVAASFKKRNKQEREGQATLAFDAQPVVEHLRRYGDQFRQLADALETTPE
                     EVQRKANSFQEFRQRPDWRKDKIACDLWTAAF"
     gene            complement(2147..>3343)
                     /locus_tag="PLY56_11670"
     CDS             complement(2147..>3343)
                     /locus_tag="PLY56_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22184.1"
                     /translation="AQIIHYELPWNPNRLEQRNGRVDRFGQREPVVYIRTLVMDERLD
                     MAMLRLLVQKAAAIRSEYGWCPPYFADEREIIRILEAKGLAPIGPDGQLAFSFEDSGG
                     EDDGLELYPRDALQRIHSESFYGQTDIQLPDVQQRLRKTYDTVASPEQVCEFVHSALS
                     RFGCIVSENDDGTYRIVVNEPNLQMADTDNTVERATFDPKVALDDPEVIVLDLGHPLV
                     RRLIDVVKLSVFRHERHYGRTAYRVTPDVEEVTALLYVLARYQVKTKPVSIIEELIPL
                     VLPVYSDRPLPSAQAEALLNAKLSPQTRTTDEVCKTLADLLERNDLEPIIARAVDERK
                     AEIVAERREWLRAMGTDGEEPMGLDTAPTSETPQASQLPEWLKGVDQLEVASTDLLTI
                     TLFYPG"
BASE COUNT          535 a          980 c         1167 g          661 t
ORIGIN      
        1 aagaaggcgg cggtccacag gtcgcaggca atcttgtctt tgcgccaatc ggggcgctgg
       61 cgaaactcct ggaagctgtt tgccttgcgc tgcacctcct cgggagtggt ctccagggcg
      121 tcggcgagct ggcggaactg gtcgccgtag cgccgcaggt gttcgacgac cggctgcgcg
      181 tcgaatgcga gggtggcttg cccctcacgc tcctgcttgt tgcgcttctt gaaggaggct
      241 gccaccgccg tgttgtcgcc ggtgacgggg gtgaaggccg cgtcggggat cccctttttc
      301 agcaccgtcg ggtccagcac gccgatcagg ctgttgccgc agcggatgtg cccgtcgagg
      361 aagttgaggg gcaggtcttg cacgcaggcg ttgatccaga gggagacttt gcacagctcg
      421 acggccatcg ggttcaggtc gacgccatag atgcagtgcg agagcacgtc gcggcgggcc
      481 ttgcggacct cggcctcggg gggatattcg tcgccggtgc gcacaatcgc cagttgctgg
      541 ccgaggaagt tggtggcggc ggtgaggaag gcgccgctgc cgcaggccgg gtcgcagacg
      601 cggagcgcca ggatcgcctc ttccgccgct tgctgctgtt cgggcttcaa tgacgttagc
      661 gtcaggggct gtttgatggg gagcttgagc gcctcgcgca cgcgctcctc aaagaccggc
      721 ttgagcgcgg actcgatcag taggttcacc agttgcgggg gcgtgtagta gctcccgctc
      781 gtcttgcgcc cggagccacg cgggtcgagg atgaagcggt tggcgggaac gggttggccg
      841 tcgatctctt cgcgggtcgt agtcacccgg ggggtgtatt ccagcaggct ctcgtagatg
      901 ctgccgatct cctccacgcc caggtcggca tagctaattc gctggagcac gcgccccttc
      961 tcaatgagcg tcagataccg gatagccttc agcaactcgc tgttgcagca ggtgaggtcg
     1021 ttcagcctac cgatcttctc cggcgagaag agcgcgccgt tataggggaa gatcccgagt
     1081 tcgggcacgc ccttttccac catgcggaag gtgaccttca ggccttccca caggtcgaga
     1141 tgggtatcgc gcgccggcac gcccttttcg gcgcgctcgc gcagcgcggt gatggagtac
     1201 tcctgggcgt agaggctgtt gcgcccgggc atcatccccc gctgctcggc atagaggaga
     1261 aagaggatgc ggtagatgac gtgcaggatc tcggagtaga agtcgcgaca ggcctggtca
     1321 tcgcgctgga gcttgccaat taattctccc tgcaagaagc cgttggccag cgcctcgatg
     1381 gcgtgcttga cgttcccctg taggtcgcgg cccacggcga ttccggtggc gatgcagtgc
     1441 tcgtagaagt gctccagggg cggcttcccc tcggcatcgg gcaggaagcg cgaggagtgg
     1501 cagagccggt agagggcgag gaagtcggag aagctgcgct cttccaaaat gttctgcaga
     1561 tcgaactcca cgtagccttt ggtgtaggtg tggtggaagt cacgcagcag gcggagcgag
     1621 atgccgttgg tgacgattcc ccaggtctcg tcgcgcgcgg cgttgaggta ggcctggagg
     1681 gtatcgtgag ggcttttggc tccgcgcccg gttccctcgc gggcatccag cgattgggcg
     1741 ggggccaccg tgtggatgat tggcgcgtgg tcgccactcc agccccggtg cgagagggag
     1801 aagcgcagct cctcgccacc ctcgataggc acggtgaccg ccgagcggag gtagtgcggc
     1861 tcgaactcca gcaggtggag gaaggggatg atccagcgcc ggcgagcagc gggcaggtcc
     1921 agctccctga cgcgggcgcg cactgtctcc cagtgctcca gggcaagatt ccaggccgct
     1981 gtgatatcct tcgccagctc ggcgggcttg ggcttttgcc catcttgggt ggtgaaggag
     2041 tccggctgcg ctttggggtg ggcgacgcgc tcctcgcgca gcgcctcgat gaactgctgg
     2101 gtgaggattc cgcccgaaac cgtgatgttc tgcgtctgtg ccatggctat cccggataga
     2161 agagagtgat cgtcaacagg tcggtggagg ccacctcaag ctggtccacg ccttttagcc
     2221 actccggcag ttgggaggcc tgtggtgtct ctgaggttgg tgcggtatcg agccccatag
     2281 gctcctcgcc atccgtgccc atcgccctca gccactcccg gcgctccgcc acgatctcgg
     2341 cttttcgctc gtccaccgcc ctggcgatga ttggttcgag gtcgttccgc tcgagtaggt
     2401 cggcgagggt tttgcacact tcgtctgttg tgcgcgtctg cgggcttaat ttggcgttca
     2461 gcagcgcctc ggcctgcgcg gagggaagcg ggcgatcgga gtaaaccggc aggacgagag
     2521 gtatcagctc ctcgataatg gacaccggct tggtcttcac ctgatagcgc gccagcacgt
     2581 agaggagcgc cgtgacctcc tcgacgtccg gggtgacgcg ataggcggtg cgcccgtagt
     2641 gtctttcatg ccggaacacg gagagcttca ccacgtcgat gagtcggcgc acgagcgggt
     2701 ggccgaggtc gagaacgatc acttccgggt catcgagggc cacttttggg tcgaacgtgg
     2761 ctctctccac cgtgttatcg gtatctgcca tttgcaggtt cggttcgttg accacgatgc
     2821 gataggtgcc gtcgtcgttc tcggagacga tgcacccgaa gcggctgagc gcgctgtgga
     2881 caaactcgca cacctgttcg ggcgaggcca cggtatcgta ggttttgcgc aggcgttgct
     2941 ggacatcggg cagctgaata tcggtctggc catagaagct ctcggagtga atgcgctgca
     3001 acgcgtcgcg cgggtaaagc tccaggccgt catcctcgcc gccggagtct tcaaaggaga
     3061 aggcaagctg gccgtctggg ccgattgggg caagaccctt ggcctccagg atgcggatga
     3121 tctcgcgctc gtcggcgaag tagggggggc accacccgta ctccgagcga attgccgctg
     3181 ctttttgcac gagcaggcgc agcatggcca tatcgaggcg ctcgtccatc accagggtgc
     3241 ggatgtagac gacgggctca cgctggccga agcggtccac gcgcccgttc cgctgctcga
     3301 gccggttggg gttccacgga agctcatagt gaatgatctg cgc
//
LOCUS       DAOSCV010000469         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18261_length_3337_cov_3.273309, whole genome shotgun sequence.
ACCESSION   DAOSCV010000469 DAOSCV010000000
VERSION     DAOSCV010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18261_length_3337_cov_3.273309"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLY56_11675"
     CDS             <1..657
                     /locus_tag="PLY56_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ22185.1"
                     /translation="AGGCSYRFLRNGQVGILMGVAKPQRPVITGCRWRPLAGVMRMKL
                     EISEIIHRVGHQVTVDINEPCPADADFRCTAPIHGKLTLTNTSHHLLVRGDLATTVVA
                     ECARCLKEVLIPVKAQIEEEFPLPKIDARGAAHWEIEGEPSTIVEDYTLDTGELARQH
                     LSLAVPMAPVCDDACQGLCPTCGKNLNEGPCECPVAEIDERLAGLRALLEESEEEGEE
                     "
     gene            804..995
                     /gene="rpmF"
                     /locus_tag="PLY56_11680"
     CDS             804..995
                     /gene="rpmF"
                     /locus_tag="PLY56_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ22186.1"
                     /translation="MALPKRKTSKSNRDSRRTHWKLKLPEVSPCPRCRQPRLAHHVCR
                     HCGYYNDRRVIEVKTEEKE"
     gene            1116..2081
                     /gene="plsX"
                     /locus_tag="PLY56_11685"
     CDS             1116..2081
                     /gene="plsX"
                     /locus_tag="PLY56_11685"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009655184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOJ22187.1"
                     /translation="MRIAVDAMGGDFAPQELVKGAVQAAEALEGAEIILVGDGDRVRA
                     ELEQYPHNRRIRVHHASQVIGMDEPPAAAVRCKSDASIVVAGDLVRSGEADALVAVGH
                     TGAAMVVAAMRIGRIDGISRPTIASPMPTRHGQAVILDAGATVDCDAHNLVQFAVMGA
                     VYAEHILGIPSPKVGLFSIGEEASKGNELVKATFPLLQNAGLNFAGNIDGKDLFRGAV
                     DVVICDGFVGNTILKVAEGLAELILERIPDPTLRREVAKGMDYSEYGGAPLLGIRGVC
                     IVGHGRSNAKAVANAIRVAAVAVEQKMVCRINTMLAERGLLNRAH"
     gene            2166..3197
                     /locus_tag="PLY56_11690"
     CDS             2166..3197
                     /locus_tag="PLY56_11690"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOJ22188.1"
                     /translation="MRDNARPVGIVGLGSYLPERVLTNLDLERMVDTSDEWIRTRSGI
                     RERRIVADDQATSDLAVEAARRALADALLEPTDLDLIIVATLSPDMPFPATANIVQHA
                     IGATGVAGFDLSAGCSGFVYGLTVGAQFVATGLYRHVMVIGADALTRLTDFTDRTTCV
                     LFGDGAGAAILGEVPEGRGFLSFTMGGDGSGASLLRMPAGGSRRPASAETVANREHYI
                     KMNGNEVFKFGVRALADGTLEALQKAGYGPQDLDLLVPHQANIRIMDAAARRLEIPWE
                     RVVSDVDRCGNTSAASVPLALDRARREGRLKDGALLAFVGFGAGLTWAACTLRWTDTP
                     AMRQQATPA"
BASE COUNT          587 a         1030 c         1118 g          602 t
ORIGIN      
        1 gcgggaggtt gctcttatcg cttcctgcgc aatgggcaag tgggcattct catgggtgtg
       61 gcgaagcccc agcgcccggt catcacaggc tgtcgctggc ggccactcgc tggggtgatg
      121 cgcatgaaac ttgaaatctc tgagatcatc caccgggtgg gccatcaggt cacggtggat
      181 atcaatgagc cgtgtccggc agacgccgat ttccggtgta ctgccccgat ccatggaaag
      241 ctgacgctga ccaacaccag ccaccacctt ctggtgcgcg gcgatctggc cacgacggtg
      301 gtcgcggagt gcgcgcgctg cttgaaggag gtgcttatac cggtcaaagc gcagatcgag
      361 gaggagtttc ccctcccgaa aatcgatgcg cgcggcgcgg cccactggga gatcgaaggc
      421 gagccaagca cgattgtcga ggattatacc ctcgatacgg gcgaactggc ccggcagcac
      481 ctctcgctgg ccgtgccgat ggcaccggtg tgcgacgatg catgccaggg cctctgcccg
      541 acgtgcggca agaacctcaa cgaggggccg tgcgaatgtc cggtggccga gattgacgag
      601 cggctggcgg gcttgcgtgc cttgttggaa gagagcgaag aagagggaga ggagtagttc
      661 gctggcacac gcagctgttc ttgcggcgca ggtaccagcc gaacgagagc cgggcccggg
      721 gattccgggc gccatccctg aagaggcatt gtcccgccgg gcgtccgagc ccggggtcat
      781 tgagttggga aaggagcttt gttgtggcgc tacctaagag gaagacatcg aaatctaatc
      841 gcgattcccg gcgaacacat tggaagctga agctgccaga ggtttccccc tgcccccgct
      901 gccgccagcc gcgcctggcg caccatgtgt gccgtcattg cggatactac aacgaccgcc
      961 gcgtcattga ggtgaagaca gaggagaagg agtagggagc gtagcgcttg cgtcttacgg
     1021 acgcggcctg gtgttcggga tgtcacacac cggcccggtt gtagtgaagc cgcgctgcaa
     1081 gcgtcttccg ttcccgggcg ttcagggggc tatgaatgcg catcgccgtt gatgccatgg
     1141 gaggcgactt cgcgcctcaa gagttggtaa agggcgccgt gcaggctgct gaagctctcg
     1201 aaggggcgga gatcatcctc gtcggggatg gtgaccgagt ccgtgcggag ctggagcagt
     1261 atccccacaa ccggcgcatc cgcgttcacc atgcgtcgca ggtgatcggg atggacgagc
     1321 cacccgcggc cgcggtacgt tgcaaatcgg acgcctcaat cgtagttgcc ggcgacctgg
     1381 ttcgctccgg agaggcagat gccctcgtgg ctgtcggcca caccggagcg gccatggtcg
     1441 ttgcggcgat gcgaatcggg cggattgatg ggatcagccg gccgaccatc gcctcgccaa
     1501 tgcccacccg gcatgggcag gccgtcattc tcgatgccgg ggccactgtg gactgcgacg
     1561 cgcacaacct ggttcagttt gccgtgatgg gcgcggtcta tgcggagcat atcctcggca
     1621 ttccctcgcc caaggtgggc ctctttagca tcggggaaga agcatccaag gggaacgaac
     1681 tggtgaaggc aaccttcccc ctgcttcaga acgccggact gaatttcgcc ggcaatatcg
     1741 atggcaagga cctcttccgg ggcgccgtgg acgtggtgat ctgcgatggc ttcgtgggca
     1801 acacgatcct caaagtggcc gaggggcttg ccgagctgat cctggagcgc atccccgatc
     1861 ccaccctccg ccgggaagtg gccaagggga tggattattc cgagtatggc ggtgcgccgc
     1921 tgttggggat ccgtggcgtg tgcatcgtcg gccacggtcg ctccaatgcc aaggcggtgg
     1981 caaacgccat tcgcgtggcg gcagtggccg ttgagcagaa gatggtctgc cgcatcaaca
     2041 cgatgctcgc tgagcgcggt ctcctgaacc gcgcccactg atctcggagc ggctgaagcg
     2101 gccgagccgc ctcctcggcg cgaccaagcc ggttggattg atccactgga acatgcggag
     2161 tgaggatgag agataacgcc agacctgtag gtattgtggg ccttggcagc tacctgcccg
     2221 agcgcgtgct gaccaatctt gacctggaac gcatggtaga cacgagcgac gagtggattc
     2281 gcacgcgcag cggcatccgc gagcgtcgca tcgtcgctga cgaccaggcc acctcggacc
     2341 tggccgtgga agccgcgcgt cgggcgcttg ccgacgcact cctcgagcct actgacctgg
     2401 atctgatcat cgtggccact ctcagcccgg acatgccctt tcccgccacg gcgaatatcg
     2461 tccagcacgc gatcggcgcc accggtgttg ccggcttcga tctcagcgcc ggctgctctg
     2521 gcttcgtcta tggcctcacc gtgggggctc agttcgtggc caccggcctc tatcgccacg
     2581 tgatggtgat cggcgccgat gcccttaccc ggctcaccga tttcacggac cgcacaacgt
     2641 gcgtcctttt cggcgatggc gccggtgcag caatcctcgg cgaggtgccg gaaggacggg
     2701 gcttcctctc gttcacgatg ggcggcgacg ggagcggcgc tagcctcttg cggatgccgg
     2761 ctggcgggtc gcgccggcca gcctccgcgg aaacggtggc caaccgcgag cactacatca
     2821 agatgaacgg taacgaggtg ttcaagttcg gcgtccgcgc tctggcagat ggaacgctgg
     2881 aagcgctgca gaaggcaggc tatggcccgc aggacctgga tctcctcgtt ccgcatcagg
     2941 cgaacattcg catcatggat gccgcggcgc gccgactgga gattccgtgg gagcgcgtgg
     3001 tgtcggacgt cgaccggtgc gggaacacct ccgccgcgtc ggtgcccctc gccctggacc
     3061 gcgcgcgccg cgagggccgt ctgaaggatg gagccctcct ggcattcgtt ggctttggcg
     3121 ctggcctcac ctgggctgcc tgcactcttc ggtggactga tactcccgcc atgcggcagc
     3181 aggcaacccc agcatagcac cctccgcgag ccaccgggtt ccgcgagctg atatgaacct
     3241 gaaccggagt acccctgcca caatgagcaa gaccgctttc attttccctg gccaaggcgt
     3301 tcagcgcgtc gggatgggcc gtgaccttta cgatgcc
//
LOCUS       DAOSCV010000470         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18273_length_3335_cov_3.317988, whole genome shotgun sequence.
ACCESSION   DAOSCV010000470 DAOSCV010000000
VERSION     DAOSCV010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18273_length_3335_cov_3.317988"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLY56_11695"
     CDS             <1..353
                     /locus_tag="PLY56_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22189.1"
                     /translation="DREEMDTSELAATLRRINSEHVGIARDARLLQEALRAFEAFGIE
                     DSLPNCHPRVAEFQNMLTVSRLIARAALKREESRGGHYRTDFPRRDDEKWRRHVVFRG
                     DVTEVRERLVGDSW"
     gene            complement(367..1026)
                     /locus_tag="PLY56_11700"
     CDS             complement(367..1026)
                     /locus_tag="PLY56_11700"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ22190.1"
                     /translation="MSDLKDDAIRYAVEQAIAYEPGVRLFDVKVLVREGVVRIQGIVS
                     NLEAKMAAERAAASVPGVRRVDNTLTVEDPRGLDDLDMGHAVDEALVEDPAVDTLEIG
                     ARVQDGTAHLIGYADDLAQEEAAIRAASRAPGIKASVSEIDISPGVDIDWVDLKNRVV
                     SALDDADDLLPYGIEVTIETDGRVVLSGSVANENARRRAIEIARTVDGAKAVVDRLQL
                     Q"
     gene            complement(1067..1276)
                     /locus_tag="PLY56_11705"
     CDS             complement(1067..1276)
                     /locus_tag="PLY56_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22191.1"
                     /translation="MRECPQCRKGHWHEVVEYGTSCHGVVVVGRPPVRCTECGMRNPP
                     VEETDRYYQCETCGYVERQPGEREL"
     gene            1897..>3335
                     /locus_tag="PLY56_11710"
     CDS             1897..>3335
                     /locus_tag="PLY56_11710"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOJ22192.1"
                     /translation="MPVRNRWTRLPVLLTLVLLAAWVCARVYRASHGMLRHLEQVVEP
                     ELERELGRDVEVGRVYVSGLRRVVVEDLVIPDGEAPESGALMRVPRASIDFGILELWR
                     HRETPAAAISSIRIESPQLSLIRNESGEWNVTSLFEGSSNVSIWKGFHGRVSVAGGRV
                     TVRDHVPGKGVITTQLCDLAISVDAGERTRLQVQASASAGNALRRVAVSGSYRLGRGD
                     LDLRAEFDDADLPFWQHYTDAAPGLPARSGKASGHVRVTQNSGGALRMVGEARAEQVV
                     MTPEPLHEPLVAKTLSIQFDGPRMTLRAEGSAGRIPITASGSLLLRQEDTLLNLRFRA
                     PRLADADARKAVRDWPVLPGVVWDAPAELQAHVTGSSEAWSAKVEGRLPRVTARGFVF
                     NNLRTDMTYHRVPRLFIVRSAQAEVSGGVVRATGRVDVKSTPSRIAFQGVVENVDLAQ
                     LPLMTGVPKQGRGNVRFQASGPVDNVHTT"
BASE COUNT          537 a         1093 c         1076 g          629 t
ORIGIN      
        1 gcgatcggga agagatggac accagcgaac tggctgccac gctgcgccgg atcaactcgg
       61 agcatgttgg catcgccaga gacgcgagac tcctgcagga ggcgttgcgc gccttcgaag
      121 cgttcggcat cgaagattcc ttgccaaact gccacccgcg cgtggccgag ttccagaaca
      181 tgctcaccgt atcccggctg atcgcgcgag ccgccttgaa gcgcgaagag agccggggcg
      241 gtcactaccg caccgacttt ccccggcgag acgacgagaa gtggcgccgc catgtggtct
      301 ttcgcggcga tgtcacggag gtgcgtgagc gcctggtggg cgactcctgg taggctctcg
      361 gctgcgctat tgcagctgca gccggtcgac gaccgccttg gcgccgtcca ccgttcgcgc
      421 gatctcgatg gcgcgccggc gcgcgttctc attcgcgacg ctgcccgaaa ggaccacccg
      481 tccatcggtc tcgatggtga cctcgatccc gtagggcagt agatcgtcgg catcatccag
      541 ggcgctcacc acgcggttct tcaggtccac ccagtcaata tccacccctg gggagatgtc
      601 gatctcgctc acgctcgcct tgatgccagg ggcgcggctc gccgcgcgga tcgcggcttc
      661 ctcctgcgcc aggtcatccg cgtagccgat caggtgggcc gtgccgtctt gcacccgagc
      721 accaatctca agcgtatcaa ccgccggatc ttcgaccagg gcttcatcca ccgcgtgccc
      781 catatcgaga tcgtccaggc cgcgcggatc ctccacggtg agcgtgttat ccacgcggcg
      841 gaccccaggc acgctcgccg ctgcccgttc ggcggccatc ttcgcctcca ggttcgaaac
      901 aatcccctgg atgcgaacga cgccctctcg gacgagcacc ttcacgtcga aaagcctgac
      961 gcccggctca taagcgatgg cctgctcgac cgcgtaccga atcgcatcat ccttgaggtc
     1021 cgacatgcca ctcctcccgt atcccatcca tgctggctgg cgcccgctat agctcccgct
     1081 cgcctggctg ccgctccacg taaccgcacg tctcgcactg atagtagcgg tccgtctcct
     1141 ccaccggcgg gttgcgcatg ccgcattccg tgcatcgaac aggcggtctt ccgaccacca
     1201 cgacgccatg gcagctcgtt ccatactcga ccacttcgtg ccagtggccc tttcggcatt
     1261 gtggacactc tctcatcgct ccactcctca ccagcgactc tccttcccgg cccgatggct
     1321 tcggaaacgg cgaggcgcgc ccggacgcgg gttcagacga ccaaggtggt ggttcgcgcg
     1381 gtgggctggg ccggaaacgg cgaggggcgc gccgtcgggt gaaacccggc tgaaccatat
     1441 ccgcgccgtg gctcgcatcc atccaggcat tgaatcccct ccccgagcaa cctttgcgcg
     1501 gaatcgttgt cctactaata gtgagttctt tcatacgggg tgcctcgccc ggggaagcga
     1561 gtaacctggg gcagctccgc atccttcatt gcgacgtgac ccatcgatca cggggactac
     1621 cttgcatgat tgcgctccat cgccggtcag cagcgtagaa tgcaactgcg ggcggccacg
     1681 agagcgctga ggtttccgct ccccgccacg ccaggagtgg ccgggatatt gcccctagag
     1741 atcagaccgc ggtcccgcca ccgaggggtg gaatacgatt taatcagtct atgaagtcga
     1801 ttattgtagg ggtcttcaac ctgattctcc atatacgggt gttgtttggg tgcatgggcg
     1861 taccggttgc cgtagagtat ccgatggagg ctgggtatgc ctgtgcgtaa ccgatggacg
     1921 cgcctgccgg tcctgctgac gcttgtcttg ctcgcggcat gggtgtgtgc tcgcgtctac
     1981 cgggcaagcc acgggatgct acgccacctg gagcaggtgg tggagccgga gctagagcga
     2041 gagctcggac gtgatgtgga ggttggacgc gtctacgttt ccggcttgcg tcgcgtggtt
     2101 gtcgaggatc tggtcatccc ggatggggag gcgccggagt ccggcgccct catgcgcgtc
     2161 ccacgcgcgt ctatcgattt cgggattctg gagctgtggc ggcaccggga gacgccggca
     2221 gcggcgatct cttcgatccg cattgaatct ccacagcttt ctctgattcg caacgagtcg
     2281 ggcgaatgga acgtgacgag cctgttcgag ggctcgagca atgtctccat ttggaaaggc
     2341 ttccacgggc gggtgagcgt tgccgggggc cgcgtcaccg tgcgcgacca tgtgccgggc
     2401 aagggcgtta tcaccacgca actgtgcgat ctcgccatct ccgtggatgc gggagagcga
     2461 acaaggctcc aggtgcaggc ctcggccagt gctgggaacg ccctccgtcg ggtcgcggtc
     2521 tccggctctt atcggctggg tcgcggtgac ctggacctgc gcgccgagtt cgacgatgcc
     2581 gacctgccgt tctggcagca ctacaccgat gcggcgccgg gtttgcccgc gcgcagcggc
     2641 aaggcgagcg gtcacgtgcg tgtcacccaa aactctggcg gtgcattgcg catggttgga
     2701 gaggcccgcg ccgagcaggt ggtaatgacc ccggagccgc tgcatgagcc cctggtggcg
     2761 aagacgctct ccatccagtt cgatggcccg cggatgacgc tgcgcgcgga gggaagcgct
     2821 gggcggattc cgatcacggc gagcggctcg cttctcctgc gccaggagga tacgctcctc
     2881 aacctgcgct tccgggcgcc tcgcctcgcg gacgcggatg cccgaaaggc ggtccgcgat
     2941 tggcccgttc tgcccggagt ggtctgggat gcaccggcag agctgcaggc gcacgtcacc
     3001 ggctcttccg aggcgtggtc cgcaaaggtg gagggccgcc tgccgcgcgt gaccgcgcgt
     3061 ggcttcgtct tcaacaacct ccgcacggat atgacctatc accgcgtgcc tcgcctcttc
     3121 atcgtgcgct cggcgcaggc ggaggtatcg ggtggcgtcg tgcgtgccac aggccgggtt
     3181 gatgtgaaat cgacgccatc ccgaatcgcc ttccagggcg tggtggagaa tgtggatctg
     3241 gctcagctcc ccttgatgac gggcgtgccc aagcaagggc gaggcaatgt gcgcttccag
     3301 gccagcgggc cggtggataa cgtgcatacc acctt
//
LOCUS       DAOSCV010000471         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18365_length_3323_cov_3.934517, whole genome shotgun sequence.
ACCESSION   DAOSCV010000471 DAOSCV010000000
VERSION     DAOSCV010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18365_length_3323_cov_3.934517"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..479
                     /locus_tag="PLY56_11715"
     CDS             138..479
                     /locus_tag="PLY56_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22193.1"
                     /translation="MDARYARGVELRINGQYDEAIQILDSILEADPGHVQARHEKALA
                     LCYSGLFDESIAEFERVREEAPDFLQARNNLGLTYAMLGMNDEAKAEFQFVLARDPNN
                     EVALKNIVYFQ"
     gene            695..1183
                     /locus_tag="PLY56_11720"
     CDS             695..1183
                     /locus_tag="PLY56_11720"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ22194.1"
                     /translation="MSALTQSDTLRQFSRYSVVGLSGFFVDFGTYWILTRCFSFWRTY
                     LLAANAIAFTLAVINNFTWHKLWTFRGQKSVEPAPDAQRAQASEPGRLRRLGGQFATF
                     ALISVIGLGLNTTILATVYRQALVQSIFGSHADLFAKVIATGVVWFWNFGANKFWTFR
                     QG"
     gene            1281..>3323
                     /locus_tag="PLY56_11725"
     CDS             1281..>3323
                     /locus_tag="PLY56_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22195.1"
                     /translation="MAALTGAFFWRALFTGAVLLPADILLRLQPWRLYSYHQFPEFRR
                     IYNPLLDAVLLFFPWRVFAARSIRAGEIPLWNPDSFCGQPFLANVSSAVLYPLNALFY
                     VIDPARAYGYVVALHIFLAGAFTFLFLRVLGLRRPAALLGGIGFMFCGFLIVWAEYQT
                     PVAATVWLPLALYFWERYARGQGRRYALYAALPLGMSLLGGHLQYAVYVLLAFGAYAL
                     WRSRLDWRSLVAAFAAGAIGLALAAHQILPSLELGRYNHRAGGQSLEGVLSAAMPAKQ
                     LITLLVPNFFGNSVDYNYWGHINFVEFCGYLGLLPLLLAAAGLWLRRDRQTWFFALLA
                     VITLLMVLGTPLYAPFYYLVPGFKQLSNPARLLGVFSFAVACLGALGADAVLGLPPER
                     RRGLAYFTGGFFLAALTAVALVYLLCAGEMASYGVAPYVERAILLFLATALVIVAAVV
                     GTAGRGRAAYLLPALAAADLFLFGMRFNPAQDPAMLYFETESLRLLRQEIRDARMIAL
                     PAPGDDFMNAMIPNCNLVVGLPEVQGGDAMYPRRYREFVEFVETRHQGKPVSTGNGLR
                     FSSIDTPGLDFLNAGYVLSGTELKSERLEHLGSPDAHLYRNRFARPRAFLVHQARVEP
                     TERVLDAMAEEGFGLDRVALLEEEPGIALTPAAGPESARIVESRNGRVRIEVEATGG"
BASE COUNT          468 a         1095 c         1069 g          691 t
ORIGIN      
        1 ccggcaaaga aatcccgtct accctcgcaa agtgccatcc ggtgtgtgcg ttgggtactg
       61 tgtgcctccg cctctctcgg tgagagcagg cgcaccgaag gggtcaggag atgggagcgc
      121 aacccgcacg agaggagatg gacgcccgtt atgcccgcgg ggtcgagctc cggatcaacg
      181 ggcagtatga cgaggcgatc cagatcctgg atagtatcct ggaagcggat cccgggcatg
      241 tgcaggcgcg gcatgagaag gctctcgcgc tttgctatag cgggctcttc gatgagtcga
      301 tcgcggagtt cgagcgggtc cgcgaagaag ccccggattt ccttcaggcc cgaaacaacc
      361 tgggcctcac ctacgccatg ctcgggatga acgacgaggc gaaggccgag tttcaattcg
      421 tgctggcgcg ggatccgaac aacgaagttg ccttgaagaa tattgtgtac ttccaatgat
      481 gcaccccagc gtggagtgga aggcacccta atatgtgcgc caagccggag cgggctgacc
      541 gcttcggctt tttggttggc ggaccggaaa cgggcttcca tcgagtggca ccgctcttgc
      601 gcattcggag accggttggg ggatgcccat gtggtcgaag gcgcggagcg acaggcaggc
      661 cgcgcgagag cctgcctggg gtgagggtaa ggtcttgtcg gcattgaccc agtcggatac
      721 gttgcgccag ttcagccgct acagcgtcgt cggactgagc ggcttcttcg tcgattttgg
      781 cacctactgg attctcaccc ggtgcttctc tttctggcgc acctacctcc tggcggccaa
      841 cgccattgcc ttcaccctgg cggtcatcaa caacttcacc tggcacaagc tgtggacctt
      901 ccgagggcag aagtccgtgg agccggcgcc agatgcccag cgcgctcagg catccgagcc
      961 gggtcggctt cgccgcctcg gcggccagtt cgcaaccttc gccctcatca gcgtgatcgg
     1021 actcgggctg aacacaacca tccttgcgac cgtctaccgg caggcgctgg tgcagagcat
     1081 cttcggatcc cacgccgatc tcttcgccaa agtgattgcc accggcgttg tctggttttg
     1141 gaacttcggt gcgaacaagt tctggacttt ccggcaaggc taggggacgc cgctcggccg
     1201 cgttgcctgc cgccccgcct ctctccgagg gggtaaagca gggctccgag cgcgcgcggg
     1261 atgccgcatg cctcggggtg ttggcggcgc tgacaggcgc gttcttctgg cgcgcgctct
     1321 tcaccggcgc ggtgctcctc cccgcggata ttctcctgcg actgcagccg tggcgcctct
     1381 actcctacca tcagttcccc gagttccggc ggatctacaa cccgcttctg gatgctgttc
     1441 tcctcttttt tccctggcgt gtcttcgccg cgcgctctat ccgtgccggg gagatcccgc
     1501 tctggaaccc cgactccttc tgcgggcagc cgtttctggc gaacgtttcg tccgcggtgc
     1561 tctacccgct gaacgcgctc ttctacgtga tcgatccagc gcgtgcctat ggctacgtcg
     1621 tcgccctaca tatcttcctg gcgggggcct tcacctttct cttcctgcgc gtgctgggcc
     1681 tccgccgtcc ggcagctctc ctcggcggca tcgggttcat gttctgcggc ttccttatcg
     1741 tctgggcaga gtatcagacg cccgtggctg ccacggtgtg gttgccgctc gccctctact
     1801 tctgggagcg atacgcccgc ggccagggaa ggcggtatgc gctctatgct gcgctcccgt
     1861 tggggatgtc gctgctaggc gggcacttgc agtatgccgt ttacgtgctc ctggcttttg
     1921 gcgcctacgc gctgtggcgc agcaggcttg actggcgctc gctcgtcgcg gccttcgctg
     1981 ccggggccat tgggctcgcg ctggccgctc accagatctt gccctcgctg gaactgggcc
     2041 gctacaacca ccgcgcgggt gggcaatcgc tggagggggt cctcagcgcc gcgatgccgg
     2101 cgaagcagct gatcaccctc ttggtcccca acttctttgg caacagcgtc gattacaact
     2161 actgggggca tatcaacttt gtcgagtttt gcggctacct gggcttgttg ccgttgctcc
     2221 tcgcggcggc ggggttgtgg ctgcgccgtg accggcagac gtggtttttc gcgcttctcg
     2281 ccgtgatcac gctcctgatg gtgctcggca cgccgctgta tgcgcccttc tactatctgg
     2341 tacctgggtt caagcagctc tccaacccgg cgcggctcct gggcgtcttc agtttcgcgg
     2401 tcgcctgtct gggggctctt ggcgcggatg ccgtgctcgg tctccccccc gagcgccggc
     2461 gcggcctggc gtatttcacc ggcgggttct tcctggcggc gctcaccgcc gtcgctctgg
     2521 tctatctcct ctgtgcgggc gagatggcca gctacggcgt cgccccctac gtcgagcgtg
     2581 ccattctgct ctttctcgcc accgcgctgg tcatcgtggc tgccgtcgta ggaactgccg
     2641 ggcgcgggcg cgcggcctac ctcctgccgg cgctcgccgc tgcggatctc ttcctcttcg
     2701 gcatgcggtt caacccggcg caggatccgg cgatgctcta ctttgaaacc gaatcgctgc
     2761 gcctcctccg gcaggagatc cgcgacgccc ggatgatcgc cctgcccgcc cctggcgatg
     2821 acttcatgaa cgcgatgatc cccaactgca acctggtggt cggactcccc gaggtgcagg
     2881 gcggagacgc gatgtacccg cgtcgctacc gggagtttgt cgagtttgtc gagacgcgcc
     2941 accaggggaa gccggtcagc acaggcaacg ggctccgctt cagcagcatc gatacccccg
     3001 ggctcgactt cctcaacgcc ggctatgtgc tcagtggcac cgagttgaag agcgagcgtc
     3061 tggagcatct cggctcgcct gatgcccatc tctaccggaa ccggtttgcc aggccgcggg
     3121 cgttcctcgt tcaccaggcg cgcgtggagc ccacggagcg cgtcctggac gcgatggcgg
     3181 aggaaggttt cgggctggac cgcgttgctc tgctcgagga ggagccgggg atcgccctga
     3241 cgcccgccgc cggccccgaa tcggcgcgga ttgtcgagag ccgcaacggc cgggtgcgca
     3301 tcgaggtgga ggcgaccggc ggc
//
LOCUS       DAOSCV010000472         3323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18369_length_3323_cov_3.160037, whole genome shotgun sequence.
ACCESSION   DAOSCV010000472 DAOSCV010000000
VERSION     DAOSCV010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18369_length_3323_cov_3.160037"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLY56_11730"
     CDS             <1..1145
                     /locus_tag="PLY56_11730"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ22196.1"
                     /translation="KVNWNCSGAPKEFVDRMRAEGRLQKTSAGEEQPWLCPSHPENFK
                     LELESMLEVVRNYDVDGIHFDYIRYPGSDNCFCDGCRQRFEESAGVKVEKWPQDARRG
                     GVHYEAYQEFRRGNITRLVKAVAEGSRAIKPWVKISAAVFSTWPGCRDSVAQDWGHWV
                     EQGYLDFVCPMNYTNYDDGYRTTVQIQRDLIAARIPFYPGVGASAPGLPVTQVIDQIQ
                     IGRAEGSDGFIIFNYAGEVAHEHVPTLGLGTTQGETTPPHNAPVIEWRVVPGSQTASG
                     AVMPDEPVQIEATVTTRGSLREAIRSAEATVSLTALDGTPVVELGKTTAGGPAARGQV
                     RLPAGLYRVVARGRMTLASGQTREFTARGPFVRVGMGWPDPDRAGN"
     gene            complement(1202..3244)
                     /locus_tag="PLY56_11735"
     CDS             complement(1202..3244)
                     /locus_tag="PLY56_11735"
                     /inference="COORDINATES: protein motif:HMM:NF026221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22197.1"
                     /translation="MRCSWFRAHGRQLHLDCHLPEFPSAAFKNFDARAFVDQLEKGRV
                     NLVALFAKCHFGNSYYNTKAGHKHQRLPQDLLMETACECRMRGIKTLAYYSLCWDKHA
                     WDENPAWRFMDAEGRTFGEDRPWGTLCMNTPYKDELVIPQLAEIAIGYPVDGFFLDIP
                     MPYIGDNLCFCAFCRRRWKVEYDIDLTPSLPRDLRARLAMRTLVSYLQEIRDLIASTD
                     PALVICMNDVGTSEVSLPVKELCEIGVWEAQPRPGDYLGQSYAARVARNDILDSQVMT
                     VRFYQSWGDMTLKPTPQLTTEFAAMIGNGVVASAGDQVNVDGTLQAPVYDAFSQAFGF
                     VADREEVLAGAESVRHAVVLLPVPDPELPLGFALGEARTGQQGPAPWRGAHQLLVESH
                     IQVDLLYSVLAEDLHRFPILILPEPGAYQPGMHERLRRYVADGGTLVAVGKSLLQGGQ
                     FHLEDVFGIRYLEPLSFDIAHFLPAEEVRGETAEIPLQVRGQVYKVRREGAQELAALI
                     YPVAQHQPPGRAFRSPPPPDDARSPFSFATLHRYGEGQAIYIAASLFEIYWRTHHHWL
                     RQFVEAVLRYADPTLPYEIEASGRVEANLMRKGSDLLLNLIHYAPGHQGRPGAIAGIE
                     RVDPLRDIACAVRCRKPERVVCEPQGQKLPFSYEEGICRFVVPELEYLAIVRICEG"
BASE COUNT          607 a         1062 c         1113 g          541 t
ORIGIN      
        1 ggaaggtgaa ctggaactgc tccggcgccc ctaaggagtt tgtcgaccgg atgcgcgccg
       61 agggccgcct ccagaaaaca agcgccggcg aggagcagcc gtggctctgc ccatcccatc
      121 cggagaactt caagctggag ctcgaatcga tgctcgaagt ggtacgcaat tacgacgtgg
      181 atggcatcca cttcgactac atccgctatc cgggcagcga caactgcttc tgcgatggct
      241 gccgccagcg tttcgaggag agcgccggtg tgaaggtgga gaagtggccg caggatgctc
      301 gccggggtgg cgtccactac gaggcctatc aggagttccg gcgcgggaac atcacccgtc
      361 tggtgaaagc ggtggccgag ggctcgcgcg cgatcaagcc gtgggtcaag atctccgccg
      421 ccgtcttcag cacctggccg gggtgccgcg atagcgtcgc gcaagactgg ggccactggg
      481 tggagcaggg ctatctcgac ttcgtctgcc cgatgaacta caccaactat gacgacggtt
      541 atcgcaccac ggtgcagatt cagcgcgacc tgatcgcggc acgcatcccc ttctatcccg
      601 gggtcggcgc ctcggccccc ggtctcccgg tgacgcaggt gatcgaccag atccagatcg
      661 ggcgcgccga gggctccgac ggcttcatca tcttcaatta cgccggcgag gtggcccacg
      721 agcatgtgcc cactcttggc ttgggcacca ctcaggggga aaccactccc ccgcacaacg
      781 cacccgtgat cgagtggcgg gtggtacctg gcagccagac tgcctccggt gccgtgatgc
      841 cggacgagcc ggtgcagatc gaggcaacgg tgacgacacg cgggtcgctg cgcgaagcga
      901 tccgctccgc cgaagccacc gtgagcctga ctgccctgga tggcacgccg gtggtggagc
      961 tggggaaaac gaccgccggt ggcccggctg cgcgtggcca ggtgaggctg ccggctggcc
     1021 tctaccgggt ggtcgcccgc ggccggatga cactggccag cggccagacg cgggagttca
     1081 ccgcccgcgg gccattcgtg cgcgtgggta tgggatggcc ggacccggat cgggccggca
     1141 actagctggt tgatatgccg cgcccgcagg tgtgggcggc ccgcgtggcc gcccaccggg
     1201 cctaaccctc gcagatgcgc acaatcgcca ggtattcgag ctctggcacc acgaagcggc
     1261 agatgccctc ctcgtaggag aaggggagct tctgcccctg cggctcgcaa acaacgcgct
     1321 ccggcttgcg gcagcgcacg gcgcacgcga tgtcgcgcag tggatcgacg cgctcgatac
     1381 cggcaatggc gcctggcctt ccttggtgtc ccggggcgta gtggatcagg ttgaggagca
     1441 gatcgctccc cttgcgcatc agattggcct ccacccgccc ggaagcttcg atctcgtaag
     1501 gcagagtggg atcggcatag cgcaacaccg cttcgacaaa ctgacgcagc cagtggtgat
     1561 gggtgcgcca gtaaatctca aagagcgagg ccgcaatgta gatcgcctgc ccctcgccat
     1621 agcggtggag cgtggcgaag gagaagggtg agcgcgcatc atccggcggc gggggcgacc
     1681 ggaaagcacg cccgggcggc tggtgctgag caaccgggta gatcagtgcc gccaactcct
     1741 gggcgccttc ccggcgcacc ttgtagacct ggccgcgtac ctgcaacggg atttccgccg
     1801 tctcgcccct cacctcctcc gcgggcagga aatgcgcgat atcgaagctg agcggctcga
     1861 gatagcgaat gccaaacaca tcctcgaggt ggaactgccc cccttgtaac agcgacttcc
     1921 cgacggctac cagcgtgccg ccatcggcaa cataccggcg caggcgttcg tgcatccccg
     1981 gctggtaggc gcccggctcg ggaaggatca ggatcgggaa gcggtggagg tcctccgcga
     2041 ggacgctgta gagcagatcc acctggatat ggctctcgac gagcagctga tgcgcgccac
     2101 gccagggcgc ggggccctgt tgcccggtgc gcgcctcgcc caatgcgaag ccgaggggca
     2161 gctccgggtc gggcaccgga agcagcacca cggcatggcg cacgctctct gcgcccgcaa
     2221 gcacctcctc gcggtcggcc acaaagccga atgcctggga aaaggcgtcg taaacagggg
     2281 cttgaagcgt gccatcgacg ttcacctggt cgcccgcgct ggcgacaacg ccattgccga
     2341 tcatcgccgc gaactcggtc gtcagttgcg gcgtgggctt gagcgtcatg tcaccccagc
     2401 tctgatagaa gcggaccgtc atcacctggg agtcgaggat gtcgttgcgg gcgacgcggg
     2461 cggcatagct ttgccccagg taatcgccag ggcgcggctg cgcctcccag acgccaatct
     2521 cgcacagctc cttcacaggc agcgagacct cggaggtgcc tacatcgttc atgcagatga
     2581 cgagtgccgg atccgtgctg gcaatcaggt cgcggatctc ctggagatag ctaaccaagg
     2641 tacgcatcgc caggcgggcc cgtaggtcac gcggcaatga gggggtgagg tcgatgtcat
     2701 actccacctt ccatcgccgc cggcaaaagg cgcaaaaaca gaggttgtct ccgatgtagg
     2761 gcattgggat gtcaaggaag aagccgtcca ccggataccc gatggcgatc tcggcaagct
     2821 gcgggatcac cagctcgtct ttgtagggcg tgttcatgca gagggtaccc cacggccgat
     2881 cctcgccaaa ggtacgccct tcggcatcca tgaagcgcca ggcggggttc tcgtcccagg
     2941 catgcttgtc ccagcagagg gaatagtagg ccagcgtctt gatgccgcgc atgcggcact
     3001 cgcacgccgt ctccatcagg agatcctggg gcagtcgctg atgcttgtgt cccgcctttg
     3061 tgttgtagta ggagttgccg aagtggcact tggcgaagag ggccacgaga ttgacgcgcc
     3121 ccttctccag ttggtccacg aacgcacggg cgtcgaagtt cttgaacgcc gcggatggaa
     3181 actcgggcag atggcagtcc agatggagtt gacggccgtg agcgcgaaac cacgagcaac
     3241 gcatctggga tctcctccgt ctggcatcat ccggggcgcg ctgctcccga gcccggccgc
     3301 aatcgccgcc aaggacgcgg atg
//
LOCUS       DAOSCV010000473         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18429_length_3315_cov_2.907055, whole genome shotgun sequence.
ACCESSION   DAOSCV010000473 DAOSCV010000000
VERSION     DAOSCV010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18429_length_3315_cov_2.907055"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..2207)
                     /locus_tag="PLY56_11740"
     CDS             complement(267..2207)
                     /locus_tag="PLY56_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOJ22198.1"
                     /translation="MKRPQLSLFDNLEPEPWPNHPEARAPQADPAAAAAEPPRHVSQL
                     SLFTEDEGGAERDLPEGFRLVDTEDALQELIRELAAAPLFAFDTETTALDPMVAELVG
                     LSFSTRPGSASYVPVGHTRGRQVERSRVLDALRPVFEDGTRPKVGHNLKFDLRVLERA
                     GVRVAGPYYDTMTLAHLVREEGSHGLKECARHYLGREMTSFDELTSRGRHPLESLPTA
                     WVARYACDDADATLSLWERLSPEIAASGLERVFQLESELLPVVAQMESDGVAIDAAYL
                     TELAADLEKQIEAKAEACYAAAGTRFNLNSPRQIATILYERLGLNATRHTTMGPSTGE
                     PALKALAGTHPLPALILEHRELSKLRSTYALGLLRSISLETRRVHPSFQQIGARSGRF
                     SCAHPNLQNLPKDSANTIRRAFAAAPGHLLVFADYSQIELRILAHYSEDPGLLAAFRE
                     GEDVHARTAAEIFRVPLDSVTSDQRQVAKGINFGLVYGMSSQGLAAQLGISQAQAERY
                     IEAYFGRYAGVRRYMAEMVARAREQGHVTTLLGRRRHVTGLDSSNPRIRGQAERQVIN
                     APIQGSAADIIKLAMVRLAPHLPAFRARMILQVHDELVIEVPEEEAEGLRRTIVSVME
                     QPPTPEFRVPLCVEVEMGSTWR"
     gene            complement(2345..2848)
                     /locus_tag="PLY56_11745"
     CDS             complement(2345..2848)
                     /locus_tag="PLY56_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22199.1"
                     /translation="MYAQDYDGMYVRYGGTLLWSEKIEPYVKNSGVFLCPNKGGWTTT
                     TPCSENNHPPESEFRQKGKGISYGYNNFTETYGEVNGVSGSQGFGLGYGYSDAQIHLP
                     ADTIAVGDAVCPRWRGKDHVDNFNSGSKSYLRHTDGANFLFVDGHVKWLKKLRYCQFD
                     PGRTADW"
     gene            complement(2787..3014)
                     /locus_tag="PLY56_11750"
     CDS             complement(2787..3014)
                     /locus_tag="PLY56_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22200.1"
                     /translation="MRRAKTRGFTLIELLVVIAIIAILAAILFPVFARARERAQSQLP
                     VEPETDRLGAQHVRARLRWDVRALWGYAPLV"
BASE COUNT          552 a         1145 c         1030 g          588 t
ORIGIN      
        1 gcccggggca agcgcttcga gcaccataca cagcgccgcc ttcaccggct cgtcccaaaa
       61 caccgtctgc cccagggcga ccagcggcac atcggcatag ttgctccgca agcgcgcggc
      121 cacctccagg gcggacggat ggagtttctc tctctcgatc ctcaaatgta tctccctcgc
      181 aggtcatcgt gcgtcatccc tcgcgcgagt ggcccggact gggtagcgcc gttccatgtc
      241 gcgccgacgg gcgcgccaat gcccccctag cgccaggtgc ttcccatctc aacctccacg
      301 cagaggggaa cccgaaactc tggcgtgggc ggctgctcca tcaccgagac gatcgttcgc
      361 cgcagaccct ccgcctcttc ctcgggcacc tctattacta gttcgtcgtg aacctgcagg
      421 atcattcgcg cccggaaagc cgggaggtgc ggtgccagcc ggaccatcgc gagcttgatg
      481 atatcggcgg cgctcccctg aatcggcgcg ttgatcacct gccgctcggc ttgcccccga
      541 atgcgcgggt tggacgaatc gaggccagtc acgtgccgcc tccggcccag aagcgtggtc
      601 acatggccct gctcgcgtgc ccgcgcgacc atctcggcca tgtagcggcg cacaccagcg
      661 tagcgcccga agtaggcctc gatgtagcgt tctgcttgcg cctgcgagat gccgagctgc
      721 gccgcgagcc cctgggagct catgccgtaa accagcccaa agttgattcc cttggcaacc
      781 tgccgctgat cagatgtcac cgaatcgagc ggcacccgga agatctccgc tgctgtgcgc
      841 gcatgcacat cctccccctc tcggaaggca gcaagcagac ccggatcttc cgagtagtgg
      901 gccagaatgc gcagttcaat ctgcgagtaa tccgcgaaga cgagcaggtg ccccggcgcc
      961 gccgcgaacg ccctccggat cgtgttcgcc gagtccttgg gcaggttttg cagattcggg
     1021 tgggcgcagc tgaagcgccc cgaccgggcg ccgatctgct gaaaactggg atgaacgcgc
     1081 ctcgtctcca gcgagatact ccgcaacagc cccagcgcgt aggtactccg gagcttgctc
     1141 agctcccggt gctccaggat gagcgcgggg agtggatggg ttccagcaag cgccttcaac
     1201 gccggctctc cggtggaggg ccccatcgtt gtgtgccgag tcgcgttcag ccccaggcgc
     1261 tcgtagagga tggtcgcgat ctgccgcggc gagttcaggt tgaaccgggt tcccgccgcc
     1321 gcgtagcagg cctccgcctt ggcttcgatc tgcttctcca aatcagctgc cagctcggtc
     1381 aggtacgcgg catcgatggc gacgccgtcg ctctccattt gcgccaccac cggaagaagc
     1441 tcgctctcca gctggaagac gcgctccagc cccgaggcgg cgatctcggg agagagcctc
     1501 tcccacaagg agagcgtcgc atcggcatcg tcacaggcgt accgggccac ccacgcggtc
     1561 gggagtgatt cgagagggtg ccggccgcga gacgtcagct cgtcgaaaga ggtcatctcg
     1621 cgcccgaggt agtggcgcgc gcactccttc aggccgtggc ttccctcctc gcgcacgaga
     1681 tgcgccaggg tcatcgtatc gtagtatggc cccgcgacgc gcacccccgc ccgctccagg
     1741 acgcgcaggt caaacttcag gttgtggccc accttcgggc gagttccatc ttcgaacacc
     1801 ggtctcagcg cgtccagcac gcgcgaccgc tccacctgcc ggccgcgcgt gtgtccaacc
     1861 ggcacataac tcgcgctccc cggcctcgtg gagaagctca agcctaccag ctccgcgacc
     1921 atcgggtcga gcgccgttgt ctcggtatcg aaggcgaaga gcggcgccgc agccagctcg
     1981 cggatcagct cttgcagggc atcctctgta tcgacgaggc ggaagccctc cgggaggtca
     2041 cgctccgcgc caccctcatc ctcggtgaag agggagagct gggacacgtg ccgtggcggc
     2101 tcagccgccg ccgcggcggg atccgcctgt ggcgcgcgcg cttccggatg gttgggccat
     2161 ggctccggct ccaggttatc gaacaggctc aactgcggtc tcttcatgcg ttcgcgggct
     2221 cccgggtcca ttatacccct gggatccttc gcgcgtccag cgcagtttcc gtcgaacgga
     2281 aaaggcccct ctccgcgggc gcggaaagag gcccaaacat cttccggccg ccaaagcggc
     2341 cgggctacca atcggctgtc cggcccgggt cgaattggca gtagcgcagc ttcttcagcc
     2401 acttcacgtg gccatccacg aagaggaagt tcgccccgtc cgtatgacgc aggtacgact
     2461 tactcccgct gttgaagttg tccacgtggt ccttgccgcg ccaccgcggg cagacggcgt
     2521 cgcccacggc gatggtgtcg gcgggaaggt ggatctgcgc gtcggagtag ccgtagccca
     2581 agccgaagcc ctgggagccc gaaacgccgt tcacctcgcc ataggtctcg gtgaagttgt
     2641 tgtacccgta gctgatccct ttgcccttct gccggaactc cgactccggc gggtggttat
     2701 tctcggagca gggcgtcgtg gtcgtccacc cgcccttatt ggggcagagg aacacgccgg
     2761 agttcttcac gtatggctcg atcttctcag accagaggag cgtaccccca tagcgcacgt
     2821 acatcccatc gtagtcttgc gcgtacatgt tgagcgccga gccgatctgt ttcaggttcg
     2881 actggcagtt ggctttgcgc gcgctctctc gcgcgcgcga agaccgggaa gaggatcgct
     2941 gccaagatgg cgatgatagc gatcacaaca agcagctcga tcagggtaaa tccccgcgtt
     3001 ttggcgcgtc gcatcgctcg ctccctcccc cggggctccc ggcgcgtgcg gggtatggcc
     3061 cacgttctca ggccctaccg aacccgtggg cgcaacgcgc actccacccc gggcgcttgg
     3121 cggtattgta gcccgggagg acatggggtg tcaagccctg gtcccggcgg cgcgcgccgc
     3181 cggccagcta ggctaccctg ttctcagaat cgggttcgtg ggcacagccc cgaaaacact
     3241 ctgccgctag cccacattat tcatcagaat aatgaaaaga agtctctctg ccaggcgcgg
     3301 cgcaggttcg tgggc
//
LOCUS       DAOSCV010000474         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18486_length_3308_cov_2.890255, whole genome shotgun sequence.
ACCESSION   DAOSCV010000474 DAOSCV010000000
VERSION     DAOSCV010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18486_length_3308_cov_2.890255"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY56_11755"
     CDS             complement(<1..391)
                     /locus_tag="PLY56_11755"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ22201.1"
                     /translation="MGRGVYAADLHVHSVLSPCGDLEMSPGRILERAAAVGLDLIAVT
                     DHNMAENGGALRTLAAGSPVAVLYGMELETAEEAHLLCLFDALEVAAAWQEYVYARLP
                     DVANDPLRFGDQVVVNEKEEIVRFESRL"
     gene            888..2186
                     /locus_tag="PLY56_11760"
     CDS             888..2186
                     /locus_tag="PLY56_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ22202.1"
                     /translation="MPAVTSSDICVGYATHPEGEGGLELVVTVDEAPFQRIPLFTSPH
                     FARLALVGKPFLAVPAAVAGACAQQGVCHQIAAVEALEAAFGIEATPLMKAHRRILLL
                     AQWIQGHALHIHFLAAPGLLGHGSSFELASAHPEVVQRGLRLRKLGAELTGIVGGRRA
                     VPLPLRIGGTPPLPNRAAIDRALRHLERGRVDALESVAFMRAMHIPPLSIPSEQIAVR
                     EEKEYPVHAGRIASTAGLDLPVSHYHQEIQRHALAAGNPLTCHARGLAPLLVGPLARL
                     NLNQDHLSTTASQALQSCGLSLPSHNPLASVVARAVELVHAVEECTHMLNHLEWRDEE
                     VRYEVTAGEGAAAVESPRGLLYHRYRVDAGGAVREAVIISPASHNVLNMEAAVRAVAS
                     QETEERGDPLRMRLAMVARSYDPCATCSRNVAGLGFCLDA"
     gene            2237..2755
                     /locus_tag="PLY56_11765"
     CDS             2237..2755
                     /locus_tag="PLY56_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ22203.1"
                     /translation="MAEAAETLDLSGIDAFIDSVGRGHGSLIPVLQKTQESFGYLPKS
                     ALAHIADRLDMPLSEVVGVATFYSQFSLTRRGRTLLRLCDGTACHVGGAGRSIELAQR
                     ALGIQPGETTPDYEFTLEVVYCLGACAISPVAVVNGQVVGRATPEKVQQILDEALEES
                     KARQEAQATASA"
     gene            2786..>3308
                     /locus_tag="PLY56_11770"
     CDS             2786..>3308
                     /locus_tag="PLY56_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22204.1"
                     /translation="MRELAEHILDIAFNSLEAGARRLRIEVTEESAADRITIRVIDDG
                     RGMDPEEARRATDPFYTTRPTRRVGLGLPMLAQAARACEGDLVVHSRSGEGTTITAWL
                     RASHVDRQPMGDMAATLVTLIAAAPEIDLEYQHEVDGRRFSFSAARARELAGETSLAH
                     PAVARWLREYLDEQ"
BASE COUNT          533 a         1179 c         1095 g          501 t
ORIGIN      
        1 gaagccgcga ctcgaagcgc acgatctcct ccttctcgtt caccaccacc tggtcgccga
       61 agcggagtgg atcgttcgcc acgtccggca ggcgggcata gacgtactcc tgccaggccg
      121 cggcgacctc cagcgcatcg aagaggcaga ggaggtgtgc ctcctccgcc gtctcgagct
      181 ccatcccata gagcacggcg accggactgc ccgcggccag cgtgcgcaac gccccgccgt
      241 tctccgccat gttgtggtcc gtgaccgcga tcaagtccag cccgaccgcc gccgcccgtt
      301 ccaggatccg gcccggagac atctccaggt cgccgcaagg cgacagcacc gagtgaacgt
      361 gcaagtcggc cgcgtaaacc ccccggccca tcgctcgccc tccaggctcc cgtgtcgctc
      421 cccctcccgc gccccattgc gttcgggatc cattcgccaa ccaggccctc ctcccctggc
      481 ggtttcccct ttggagatct tgctcagagc acgcgctgct ggccaggcgc gcggcagccg
      541 ggcgcatgaa agagagcgat cgtgaaaatc ggtgcaaacg ccacgcatac gcgcaccggc
      601 cggcacctcg caaggggaga cgccggcccg gccgcactag aagcgcacgc acgcgcgcca
      661 gcccccggaa tcactaggca tcgcgctcat gtgatacgtg attcctctcg ttgagatacg
      721 ggtatccctg gttgacacct gccatgaaaa attggggtta gccccgattg acgcggatag
      781 ttactgatgt tatagtcatg gcaccgcaca gcagcccgga tcgccctgcc tccgaggctg
      841 ctcgaggtgc taccgacgcg gtcaccgcgc gccaggaggg cagtccaatg cccgctgtca
      901 cctccagcga tatctgtgtc ggttatgcca cccatccgga aggagagggg ggcctcgagc
      961 tcgtcgtcac ggtggacgag gcgcctttcc agcgcatccc tctcttcacg tcgcctcact
     1021 tcgcccggct cgcgctggtc ggaaagccat tcctcgccgt gccggccgca gtcgcgggcg
     1081 catgcgccca gcagggggta tgccaccaga tcgcggcggt ggaggcccta gaggcggcct
     1141 ttggaatcga ggccacgccc ctgatgaagg cgcaccggcg catcctcctc ctcgcgcagt
     1201 ggatccaagg ccacgcgctc cacatccatt tcctggcggc gcccgggctt ctggggcacg
     1261 gcagctcctt cgagttggcg tccgcacacc cggaggtcgt gcagcgcggg ctccggctta
     1321 gaaaactggg cgcggagctc accgggatcg tgggcgggcg gcgcgcagtg cccctccccc
     1381 tccgcatcgg gggaacccct ccactcccca accgggcggc catcgaccgc gcgctccggc
     1441 acctggagcg agggcgagtg gacgcgctgg agagcgttgc cttcatgagg gcgatgcaca
     1501 tccccccgct ctccatcccc agcgagcaga tcgccgtgcg cgaggagaag gagtacccgg
     1561 tccatgccgg gcggatcgcc tcgacggcgg ggctcgacct gcctgtctca cactatcacc
     1621 aggagatcca gcggcacgca ctcgccgctg gcaatccact cacctgtcac gcgcgcgggc
     1681 tggccccgct cctcgtcggg ccactcgcgc gcctgaacct gaaccaggac catctgagca
     1741 ctacggcatc ccaggcgtta caatcgtgcg ggctctcgct ccccagccac aacccgctcg
     1801 cctccgtcgt cgcgcgcgcc gtggagctgg tgcacgcggt ggaagagtgc acccacatgc
     1861 tcaaccatct ggagtggcgc gacgaggagg tgcggtatga ggtgaccgcc ggcgaaggcg
     1921 cagcagccgt cgaatcgccg cgcggcctgc tctaccaccg ctaccgcgtg gatgccggtg
     1981 gcgcggttcg cgaggcggtg atcatctcgc ccgcgtctca taacgtgctc aatatggagg
     2041 ccgccgtgcg cgccgtcgct tcgcaggaga ctgaggagcg gggagacccc ctgagaatgc
     2101 gccttgcgat ggtggcgcgc agctatgatc cctgcgcgac ctgctcgcgc aacgtcgccg
     2161 gccttggctt ctgtctggat gcctgagccg gggcgcaggc gtgcggcccg gaggcgggcc
     2221 gcaggaagga gtggccatgg cggaagcagc ggaaaccctc gatctgagcg ggatcgacgc
     2281 cttcatcgac agcgtcggga gagggcacgg ctcgctcatc ccggtccttc agaaaactca
     2341 ggagagcttt ggctacctgc caaagagcgc actcgcacac atcgctgacc ggctcgatat
     2401 gcccctatcg gaagtcgtgg gcgtcgccac tttctactcg cagttctcgc tcacgcgccg
     2461 cggccggacg ctcctccggc tgtgcgatgg cacggcgtgc catgtcggcg gcgccggccg
     2521 gtcgatcgag ctggcgcagc gcgcgctcgg catccagccg ggcgagacga ccccggatta
     2581 cgagttcacc ctggaggtgg tctactgcct cggcgcgtgc gccatctcgc cagtcgcggt
     2641 ggtgaacggt caggtggtcg ggcgggccac gccggagaag gtgcagcaga tcctcgacga
     2701 agcccttgag gagtccaagg cgcgccagga ggctcaagcc acggcgagcg cgtgaccgcc
     2761 tggcagaagg gatcggggca cagcgatgcg cgagctggcc gaacacatcc tcgatatcgc
     2821 cttcaactcg ctcgaggcgg gcgcccgccg gcttcgcatc gaggtgaccg aggagagcgc
     2881 agctgaccgg atcacgatcc gcgtcatcga tgacgggcgc gggatggatc cggaggaggc
     2941 gcgccgggcg accgatccct tctacacaac gcgccccacc cgccgggtgg gccttggcct
     3001 tccgatgctg gcgcaggccg cgcgcgcgtg cgagggcgac ctcgtggtcc actcgcgctc
     3061 cggggaaggc accacgatca ccgcctggct gcgcgccagc cacgtcgacc gccagccgat
     3121 gggcgacatg gcggccacgc tggtcacgct gatcgccgcg gcgccggaga tcgacctgga
     3181 gtatcagcat gaggtggacg ggcgccgttt cagcttcagc gccgcgcggg cgcgagagct
     3241 ggcgggcgaa acctcgctcg cccacccggc agtcgcgcgc tggctgcgcg agtatttgga
     3301 cgagcagc
//
LOCUS       DAOSCV010000475         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18510_length_3305_cov_2.775077, whole genome shotgun sequence.
ACCESSION   DAOSCV010000475 DAOSCV010000000
VERSION     DAOSCV010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18510_length_3305_cov_2.775077"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(357..752)
                     /locus_tag="PLY56_11775"
     CDS             complement(357..752)
                     /locus_tag="PLY56_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22205.1"
                     /translation="MDEEREERRDEDDSVQLFTTEDIRDADDTLTIADVRGPEDLLIP
                     IIEDERARRELLDRRRESWGWPPDEDEEVNPVGSYESWNQSGQSLDAAGAGMTGDSGS
                     IGLTGTGFENDLEIQGEPEKAQPNREGEE"
     gene            complement(789..1502)
                     /locus_tag="PLY56_11780"
     CDS             complement(789..1502)
                     /locus_tag="PLY56_11780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ22206.1"
                     /translation="MIPVRDDVPSISRPVVMYAIIVVNVLVYLYMLAIGPRGVGEVVS
                     SFGVVPVLFTRDPAGTDLLAVYPTLLTSIFLHGGLLHLLVNMWYLWIFGDNVEDRMGH
                     GKFLIFYLMGGVVANLAHIGLNPLSPIPSIGASGAVAGVLGAYLVLFPGARIITIVPF
                     IFVFAIQLPAGLVLGVWFIIQLWSGLGSIGVDGTAGGIAYWAHIGGFLFGLLVVRFLA
                     PRRRRRMRREWEPRERPSW"
     gene            complement(1499..1714)
                     /locus_tag="PLY56_11785"
     CDS             complement(1499..1714)
                     /locus_tag="PLY56_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22207.1"
                     /translation="MTGVPEEVGEQDVWPDDFPTWGPVEYSDPAEIQAPHDHLGDPTR
                     RKHPTILSAEGEAEAPATELPDRDIGG"
     gene            complement(1740..1997)
                     /locus_tag="PLY56_11790"
     CDS             complement(1740..1997)
                     /locus_tag="PLY56_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22208.1"
                     /translation="MAIQPYEELIARAQELRERLERMQRWFEENAGNADAEGLRWRKE
                     RFEAAKREYEELLQVIRAYGKGGGAARTADRGHRDAIEEAE"
     gene            complement(2296..>2748)
                     /locus_tag="PLY56_11795"
     CDS             complement(2296..>2748)
                     /locus_tag="PLY56_11795"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ22209.1"
                     /translation="LAARVVPPAARRTEPAIHSLPGPRPEVSLVLDEMTVPARVCLIE
                     GNAYVPVRPLVECLDGEALWDAQARRVTLWCRGREVQLVPGSAVGTLNGRSIRLWRPP
                     TLDGGRTILPARTFGQILDLSVAWDAVRRIVQMGLLPLRQRPEESLAG"
     assembly_gap    2749..2758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2759..3117)
                     /locus_tag="PLY56_11800"
     CDS             complement(<2759..3117)
                     /locus_tag="PLY56_11800"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ22210.1"
                     /translation="MRLLSLLSGILLAASLLPAQVSAQSWNALRLISPRDGAVVSGTV
                     TVTAEATPDLELSFVVFGIDGERPMATNARPFTWAWDTRAHPDGPHLLFVEAHGPGGV
                     VATAGPVRVIVRNQPVVS"
BASE COUNT          584 a         1172 c         1007 g          532 t
ORIGIN      
        1 tggaagccca ctgccgcccc cactgccgca gtcgcgcctg ccaagccgaa gagctccacg
       61 agcgcgcctg ccaagccgaa gagctccacg gccgcgcccg ccaagcagaa aagccccacg
      121 agcgcccggg tacgcatccg tcccaagcag tgattagggc tatggacctg cctgcactgg
      181 gcggcccggc cgtcgccctg ctcgacgatg ccggtcccag agcgaagcca tcggtttctc
      241 ctgacgctcc tggcggcccg gggcgcgcgg agaccgctcc ggggtggagc gcggtcgagg
      301 ccggtcgctg agggtgctcg ctgtggccgg cgttatagcg ccggctgctt cgcgtctcac
      361 tcctcgccct cccggtttgg ctgggctttc tccggttccc cctggatctc cagatcattc
      421 tcgaatccgg tcccagtcag cccgatgctt ccagagtcgc cggtcatgcc tgccccggcg
      481 gcatccaggc tctggcctga ttggttccag ctctcgtagc tccccacggg gttcacctct
      541 tcgtcttcat caggcggcca gccccagctc tctcgacgac ggtcgaggag ttcccggcgt
      601 gccctctcat cctcgatgat agggatgagg agatcctccg gacccctcac atcggcaatc
      661 gtgagcgtgt cgtccgcatc gcgaatgtct tccgtcgtga acagctggac gctgtcatcc
      721 tcatccctgc gctcttccct ctcctcatcc atctcccgct ctccccaaac gaccgcgcaa
      781 gacccgaatc accatgatgg cctctctcgc ggctcccatt cgcggcgcat ccgccggcgc
      841 cgccggggcg cgaggaagcg cacgaccagc aatccgaaga ggaacccgcc gatatgcgcc
      901 caataggcaa tcccaccggc ggtgccatcc acgccgatgc tccccaatcc gctccatagc
      961 tggatgatga accagacgcc caggaccaac cccgcgggca gctgaatggc gaacacaaag
     1021 atgaatggca caatggtgat gatccgtgcc ccagggaaga gcacgagata cgctcccaac
     1081 acccccgcca ctgccccact cgcgccgatg ctgggaatag gcgatagcgg attcagcccg
     1141 atgtgcgcca ggttcgccac cacgccgccc atcaggtaga agatgaggaa cttgccatgc
     1201 cccatgcgat cctcgacgtt atcgccgaag atccaaaggt accacatgtt gacaagcagg
     1261 tgcagcaagc caccatgcag gaagatcgag gtcaataagg tcggatagac ggcgaggagg
     1321 tccgtgccgg ctgggtcgcg cgtgaaaagg accggtacca ctccgaagga gctcaccacc
     1381 tcgcccacgc ccctcggtcc tatcgctagc atgtagagat agaccaggac gttgaccacg
     1441 atgatggcgt acatcacgac gggccggctg atggacggca cgtcgtcacg gaccgggatc
     1501 atccgccgat gtccctatct ggcagctcgg tggcgggcgc ctccgcctct ccctcggcgc
     1561 tcaggatcgt gggatgcttg cgccgggtcg ggtcgcccag gtggtcatga ggcgcctgta
     1621 tctccgccgg atcgctgtac tcgacggggc cccacgtggg gaaatcgtcg ggccatacat
     1681 cctgctcgcc gacctcctca ggaacgccgg tcaccctccc gatctccttc tcgcgctctt
     1741 cactcggctt cctcgattgc atccctatgc ccccgatccg cggtacgagc ggcaccaccg
     1801 cccttcccat aagcccggat gacctgcagc agctcctcat attcacgctt ggctgcctcg
     1861 aaacgctcct tgcgccagcg gaggccctca gcatcggcat tgccggcgtt ctcctcgaac
     1921 caccgctgca ttcgctccag ccgctcgcgc aactcctgcg cgcgcgcaat gagctcctca
     1981 tagggttgta ttgccattcc ggcactcctt accctccgcc ggggcgtgca tagagtcgcc
     2041 gaagcattcc cccagggagt gccgatccaa acgcggaacc ccgaaccgca tcatgccagg
     2101 cgcagcaagg cctctccagc tcgaggccgc acggttccgg ggagtgaggt ggcccgggcg
     2161 ggcagggtcg agcgcacccg ggccgatatc gattccgttg caaaaagagt ctggggtgcc
     2221 atgcaggggc ggccgcaggc atccgagagg gcatgcgatg caaggcgtcc cttcctggca
     2281 tcagacggag taggattacc cggcgaggct ctcctccggg cgctgccgca ggggaaggag
     2341 acccatctga acgatgcgcc gtaccgcatc ccaggcgacg gagaggtcca gaatctgccc
     2401 aaacgttcgg gccggcagga tcgtccgccc gccatcgagg gtaggtgggc gccagaggcg
     2461 gatgcttcgc ccgttcagag tgcccacggc gctgcccggg accaactgca cctcgcgacc
     2521 ccggcaccag agagtcaccc tccgggcctg agcatcccaa agagcctccc cgtccaggca
     2581 ctctacgaga gggcgcaccg gcacataagc gttgccctcg atcaggcaca ctcgcgcggg
     2641 cacagtcatc tcatcgagca ccaggctcac ctccggccgc ggccctggca gcgagtggat
     2701 agcgggctcc gtgcgccggg ccgccggggg cacgacccgc gcggccagnn nnnnnnnnga
     2761 gaccaccggc tggttgcgca cgatcacgcg gaccggcccc gccgtggcca cgacgccgcc
     2821 tgggccgtgg gcttctacga agaggagatg cgggccatcc gggtgggcgc gcgtatccca
     2881 tgcccaggtg aacggccggg cattcgtggc catcgggcgt tcgccatcga tgccaaagac
     2941 cacgaaggag agctccagat ccggagtcgc ctcagcagtg accgtcaccg ttccgctcac
     3001 gacggcgcca tcgcgcgggg agatgaggcg cagcgcgttc cagctctgtg cagatacctg
     3061 ggctggcagg agggatgctg ccagcaggat gcctgagagt agcgatagca ggcgcattgg
     3121 gctgcctcct ctcgtgggga aagcctgcgt gttgcgcccc agtataacag cgcctctccg
     3181 gtatgtcaag gcgctctccg cggtaccctg ggattccgac ataaggagaa ggctgcccgc
     3241 acgcgcggag ccagctaccg ccgttttgag acatctggga gtatccccaa cccagcccgg
     3301 caggg
//
LOCUS       DAOSCV010000476         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18514_length_3304_cov_3.352108, whole genome shotgun sequence.
ACCESSION   DAOSCV010000476 DAOSCV010000000
VERSION     DAOSCV010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18514_length_3304_cov_3.352108"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="PLY56_11805"
     CDS             <1..172
                     /locus_tag="PLY56_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ22211.1"
                     /translation="EAGEAEGIYLATFDLERLRAYREREAFGDAYRRPRLYGRLVETT
                     VYPPFIRGDATR"
     gene            188..2716
                     /locus_tag="PLY56_11810"
     CDS             188..2716
                     /locus_tag="PLY56_11810"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ22212.1"
                     /translation="MFKTLAAIGLLLCALASPAAAANLVLVEDGQARAQVVIPDDASD
                     RVRDLAGDFARVLARMSGARLPVVKESVSDPTQARVLIGACRASAGLDADPGAIRGDE
                     AYAGYVIACDGKNLVLRGNTPAGTTNAVYGFLQDELGVRWFLPTELFEVVPTRRTVAV
                     PEMRRVATPSFVCRLGSASWNPEALAWSRRNRWDTHEGGWEIPYAAGFRHWMYAVFPP
                     SKYGKSHPEIYPLINGKRAIPSKDGEQLAQPCTGNPETVRIAIAEVNAYLDAHPECHT
                     YPFGINDNNTWCECDLCKAQDVERPLYRGRKIYSDRWYTFVNAVARGVRARHPDKFIG
                     CFAYAGVELPPIRIERMEPNVFINLTQDTAQYFDSAYRKVDYDLIRAWQQKCDHVGKY
                     DYYGLGALAPRVFPHLLARDLKAIHAMGVRAFHSELYPYWAHMGPMLYVASRLLWDVR
                     LDPDRLLREFYSAFGPAAGEIRAFYETHEAAWMSQKTGKWFGGLGSAGEQMDFYTPAR
                     AARAAEHLRRAKALATDETTQARVDFIARSYAYPDLLLRGWTTARAIGEAAPRRPAEA
                     RALAGRVETLLEALDGEETSWKKSIADDPLADRWYQNGGRPQIRSQWRSRVQTAMLDG
                     LHALASWEKSPAGQKAAPETRALFDRIAAHPQAGFLWKAMRGTLALGPNLLPNPGFEE
                     AKGGQLGPTGPDWKSTSAAAGWSTWQENPHEGRFFLDTNEKHGGNAAGAFQGGGCLCY
                     ITTVPIETGKRYLAQVWVRKPVAREGTAVALEIRWNDAAGRWYNGAANLRLDARKAGV
                     WEQLQIPFMAPPGAARVVILLTGYGIEKEDVVRFDNAFLGEARE"
BASE COUNT          554 a         1104 c         1158 g          488 t
ORIGIN      
        1 agaagcggga gaggccgagg ggatctacct ggcgaccttc gatcttgagc gcctgcgcgc
       61 ctatcgggag cgcgaggcgt tcggcgatgc ctaccgccgc ccaaggctct acggccggct
      121 ggtggaaacg accgtttacc cgccattcat ccgcggtgat gcgacccgct gacaggagga
      181 gacgccgatg ttcaaaacgc ttgccgccat tgggcttctg ctctgcgcgc tggcatcccc
      241 agccgcggca gccaacctcg tcctcgtaga agacggccag gcgcgggcgc aagtggtcat
      301 tccggacgat gcgagtgacc gcgtgcggga tctcgccggt gacttcgcgc gcgtcctcgc
      361 gcggatgagt ggcgcccggc tccccgtggt gaaggaatcg gttagcgatc cgacccaggc
      421 gcgtgtcctg atcggtgcgt gccgggcatc ggcgggcctg gacgcggatc cgggcgccat
      481 ccgaggcgat gaagcgtacg ccggctacgt gatcgcgtgc gacgggaaga acctggtgct
      541 gcgcgggaac acgccggccg gcacgacgaa cgccgtctat ggcttcctcc aagatgaatt
      601 aggagtgcgc tggttcctgc ccacggagct gttcgaggtg gtgccaacgc ggcgaacggt
      661 ggcggttccc gagatgcgcc gggtggccac gccgagcttc gtttgccgtc tgggaagcgc
      721 gtcatggaac cccgaggcgc tggcgtggag ccggcgcaac cgctgggata cccatgaggg
      781 cggctgggag atcccctacg ccgcgggctt ccgccactgg atgtatgccg tcttcccccc
      841 gagcaagtat ggcaagagcc acccagagat ctacccgttg atcaacggca agcgcgccat
      901 cccctcgaaa gatggcgagc aactcgccca gccatgcacc ggcaatccgg agacggtgcg
      961 gatcgcaatc gcggaggtaa acgcctatct cgacgcccat ccagaatgcc acacctaccc
     1021 cttcgggatc aacgacaaca acacgtggtg cgagtgtgac ctctgcaagg cgcaggacgt
     1081 agagcggccg ctctaccgcg ggcgcaagat ttactccgac cgctggtaca ccttcgtcaa
     1141 cgccgtggcc cgcggggtcc gcgcccggca cccggacaag ttcatcggct gcttcgccta
     1201 cgcgggcgtc gagctgccgc ccatccggat cgagcggatg gagccaaacg tcttcatcaa
     1261 cctcacgcag gatacggcgc agtacttcga ctccgcctat cgcaaagtcg attacgacct
     1321 catccgcgcc tggcagcaga agtgtgatca cgtggggaag tacgactatt atggccttgg
     1381 ggcactggcg cctcgtgtct tcccccacct gcttgcccgc gatttgaagg caatccacgc
     1441 gatgggcgtg cgcgccttcc acagcgagct ctatccctac tgggcacaca tgggcccgat
     1501 gctctacgtg gccagccggc tcctctggga cgtccggctc gaccccgacc ggctgttgcg
     1561 cgagttctac tccgctttcg ggcccgccgc gggcgagatc cgggcgtttt acgaaacgca
     1621 cgaggcggcg tggatgagcc agaagaccgg gaagtggttt ggcgggctcg gcagcgccgg
     1681 ggagcagatg gatttctaca cgccagcccg agcggcacgc gccgcggagc atctgcgccg
     1741 cgcgaaagcc ctggccaccg acgagacgac ccaggcgcgt gtcgacttca tcgcccgtag
     1801 ctacgcctat ccggacctcc tcctgcgcgg ttggaccacc gcccgggcca ttggcgaggc
     1861 cgcgccacgc cgcccggcgg aagccagggc gctcgccggg cgcgtggaga cgctcctcga
     1921 ggcattggat ggcgaagaga cgtcgtggaa gaagagcatc gccgacgacc ctctcgcaga
     1981 ccggtggtat cagaatggcg ggcgcccgca aatacgcagc cagtggcgct cgagggtcca
     2041 gaccgctatg ctggacgggc tgcatgccct ggcaagctgg gagaagagcc ccgcgggcca
     2101 gaaggccgcg cctgagacgc gcgcgctgtt cgaccggatc gccgcgcacc cgcaggcggg
     2161 tttcctctgg aaggcgatgc ggggcacgct ggcgctcggc cccaacctgc tccccaaccc
     2221 ggggttcgag gaggcgaagg ggggccagct cggcccgacc ggccccgatt ggaagagcac
     2281 cagcgcggcg gccggctgga gcacctggca ggagaaccca cacgagggcc gcttcttcct
     2341 cgacacgaac gagaaacatg gcgggaacgc cgcgggcgct ttccagggcg gcggctgcct
     2401 ctgctacatc accacggtgc caatcgagac gggcaagcgc tatcttgccc aggtatgggt
     2461 ccgcaagccg gtcgcgcgcg agggcaccgc ggtcgcgctg gagatccgct ggaatgacgc
     2521 cgccgggcgc tggtataacg gcgccgcgaa cctgcgcctg gatgcccgga aggccggcgt
     2581 ctgggagcag ctgcagatcc ccttcatggc gcccccgggc gccgcgcgcg tcgtcatcct
     2641 cctcaccggc tacgggatcg agaaggagga cgtggtccgc ttcgacaacg ccttcctcgg
     2701 cgaagccaga gagtgacaga ctatcggagg ggacggcgct ggcggcgata ccacggatga
     2761 aggggacgga tgggccgggt ggtcgcaccc ggggggtgta ggccatgccc gcgcgtggcg
     2821 gtacatcccc gcgctgcttg tgagcaaagc cctgccaggc tgggttgatc tacactccca
     2881 ggtgacctgg agcagcgggg atgggacggg gagtcccgaa agggctttgc cgggtagcag
     2941 cgcggagctt cgagctccgc gcgcccggct gttcgccctg ccccagcgga aacggatggg
     3001 ctaggcatgc ccgcccgggg ttgcgcgctc catccggggg gcaccgtgag acgcggtgcg
     3061 gtagctcccc ttccctggcg ccggcgatga gccaaagggt agggaaggag ccggggggca
     3121 gggaaagaag ccgtcggtcc gggcgattgc ccgcgcgttc ctgcgggggc ggcaggagcg
     3181 caccgccggt cgcgagtccc aggcggttct ggccaaggcg cgcgccgaat cgctcaccgc
     3241 gctggagaag atgccgccgc tggtctcgcg cgccgacctg cctcccgcgc cggtcccgcc
     3301 gatg
//
LOCUS       DAOSCV010000477         3292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18610_length_3292_cov_2.902070, whole genome shotgun sequence.
ACCESSION   DAOSCV010000477 DAOSCV010000000
VERSION     DAOSCV010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18610_length_3292_cov_2.902070"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLY56_11815"
     CDS             <1..444
                     /locus_tag="PLY56_11815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOJ22213.1"
                     /translation="AESCVYALLFGGVINFLLGSLFRIPFLAPGMESLDAPARFILSL
                     GAGLYEELVFRVILVSALFFFLRQGMGARPVPAYIAAVFLGAFLFSAYHYVGSMADAF
                     SLHSFLYRFLAGLILNVLFIARGFGIVVYTHALYDVLVTVLLPGG"
     gene            complement(481..756)
                     /locus_tag="PLY56_11820"
     CDS             complement(481..756)
                     /locus_tag="PLY56_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22214.1"
                     /translation="MAIAIGTGVFLIFIGRLVYYRVYRWLQEQGRLRSKDPDHFGIFL
                     LTTGAAIAIWFALDAAMQWQTPFHWRVAAILAGFLLADGLMAGLGRN"
     gene            937..1785
                     /locus_tag="PLY56_11825"
     CDS             937..1785
                     /locus_tag="PLY56_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22215.1"
                     /translation="MKISATTVMLPEYDLVQTCQLLQRLGFDGVEWRVRRLAPGQENA
                     APSAWGRHLTDLSPENILSRAEEVKRVCADHGLGIAGFASACSATDLEQVRLLAEGAQ
                     ACGCPAIRLSCPRGYTGEVNYHELYQEAVDAYGQALEITRKYGVKVLVEMHGGTIHPS
                     ASLAYRIVSNFPATDIGVIYDPQNMVRDGFETIPLAIQLLGDYLAHLHVGAHRPEPAE
                     VDEKGTQKWKWRAVPMGMGLYDFPKLFECLRAVNYSGYISIEDFAGDRTAEEKLSSGI
                     AYLRSL"
     gene            complement(1856..2938)
                     /locus_tag="PLY56_11830"
     CDS             complement(1856..2938)
                     /locus_tag="PLY56_11830"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ22216.1"
                     /translation="MQLASLGLIGAGEGRWVHPLCKPLATEKNGPFVQMPNGSLALID
                     SEGIRFSRDEGATWFDARPIAPGVNLGHVGHVGQFLCTRNGVLVLLFLNFEGYKFAWD
                     DAAGKPSEDSRLELWSIRSLDGGATWTDRQRILGGYNADFTGFIQTRDGCLVATVEHL
                     ASDPARWVVCSFWSDDDGQHWQRSNWVDLGGHGHHDGATEPGVVELGDGRLLMLIRTN
                     LDRFWYAFSEDGGRHWRTIRPSPLDASSAPGFILRLRSGRLAVAWNRLCREGAEEVPR
                     IAAPSAASQVAASWHREELSLALSEDEVVIAREPGGQLAYPYLFERRPGELWLFTRYT
                     WDRGHKPAPPLRVRIDEAELWSIARS"
     gene            complement(2972..>3292)
                     /locus_tag="PLY56_11835"
     CDS             complement(2972..>3292)
                     /locus_tag="PLY56_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22217.1"
                     /translation="RAGAETAGPRQTLYVWATTFDTQQDAREFREAFAQVLAERIGFE
                     PQDVTELRALPNDHWGRGWLSNECLTAVLGRGNDVWVLSGIPEKAFPAVLEKARQLTR
                     QEKK"
BASE COUNT          534 a         1116 c         1049 g          593 t
ORIGIN      
        1 gccgagagct gcgtctacgc gcttctcttc ggcggcgtga tcaacttcct gctcggctcc
       61 ctcttccgca ttcccttcct ggcgccgggc atggagtcgc tggatgcgcc ggcgcgcttc
      121 atcctttcgc tgggggcggg gctgtatgag gagctagtct ttcgcgtgat cttggtgtcg
      181 gccctcttct tctttctgcg ccagggaatg ggtgccaggc cggtgcctgc ctacatcgcc
      241 gcggtcttcc tgggtgcgtt tctcttttcc gcctaccact acgtgggctc gatggccgat
      301 gccttctcgc tccactcctt cctctaccgc ttcctggccg gcctcatcct caacgtcctc
      361 ttcatcgcgc gagggttcgg catcgtcgtc tatacccatg cgctctatga cgtcctcgtg
      421 accgtgctcc tcccaggcgg gtagcgagag cggaccggcc gccagcgagc atcccgctgt
      481 tcagttccga cctagacccg ccataaggcc gtcggcgagc aggaaccctg cgagaatcgc
      541 cgccacgcgc cagtggaatg gcgtctgcca ctgcatcgcc gcatccagcg cgaaccagat
      601 ggcgatcgcc gcccctgtgg tcagcaggaa gattccgaag tgatcgggat ccttgctacg
      661 cagccgccct tgctcttgta gccaccggta gacgcggtaa tagaccaagc gtccgatgaa
      721 gatgaggaag acccctgtgc caatcgcgat tgccatgcgt tatgctccca agtgccagga
      781 caccggcgca gcgcggttcg ctcccgaaag tggcccctgc agcggccaac cggtgtgcca
      841 aacgccgaga ggaaggtgcc tcctggccgc gcggagaagg cagatccccg gatccgtctc
      901 gatacgggcg gccacttccc gactggagga gaaatcatga agatatcggc aacgacggtg
      961 atgctgccgg agtatgatct ggtgcagacg tgccagctgc tgcagcggct tggcttcgac
     1021 ggcgtagagt ggcgcgtgcg ccggctcgcg cccggccagg agaacgccgc gcccagcgcc
     1081 tggggccggc acctcacgga cctctctcct gagaacatcc tctcgcgcgc cgaggaagtg
     1141 aagcgtgtct gcgcggatca tggcctgggc attgctgggt ttgcttcggc ctgctccgcg
     1201 actgacttgg agcaggtccg tcttctggcc gaaggcgcgc aggcgtgcgg ttgcccggcc
     1261 atccggttga gctgcccgcg tggctacacc ggcgaggtga attaccacga gctgtaccag
     1321 gaggccgtgg acgcctatgg ccaggcgttg gagatcaccc ggaaatacgg cgtgaaggtc
     1381 ctggtcgaga tgcacggggg caccatccat cccagtgctt ccctggccta tcgcattgtc
     1441 tccaacttcc ccgcgaccga tatcggcgtg atttacgacc cgcagaatat ggtgcgcgac
     1501 ggcttcgaga cgattccgct tgccatccag ctgctggggg actacctggc ccacctgcac
     1561 gtcggtgcgc accgccccga gccggccgag gtggatgaga agggcactca gaagtggaag
     1621 tggcgcgccg tgccgatggg catgggcctc tacgatttcc cgaagctttt cgagtgcctg
     1681 cgggccgtga actactccgg ctatatctct atcgaggact tcgcgggaga tcgcaccgcc
     1741 gaggagaagc tctccagcgg catcgcctac ctgcgctcgc tgtaaactca gtgttcactc
     1801 ctccttctcc ctccccatct tgggggaggg aggggatggg ggattccggc cgctcctatg
     1861 agcgggctat cgaccacagc tccgcttcgt cgatgcgcac acgcagtggc ggcgcgggct
     1921 tgtgcccccg atcccacgtg tagcgcgtga agagccacaa ctcgccgggc cgccgctcga
     1981 agagataggg ataggcgagc tgcccgcctg gctcccgcgc gatgacgacc tcatcctcgg
     2041 agagggcgag tgagagctcc tcgcgatgcc aggacgcagc cacctgcgac gcggcgctcg
     2101 gggcggcaat ccggggcacc tcctccgcgc cctcgcgaca gagccggttc cacgccaccg
     2161 caagccgccc actgcgcagc cggaggatga aaccgggggc gctgctggca tccagcggac
     2221 tgggccggat tgtgcgccag tgccggcctc catcctcgct gaaggcgtac cagaagcggt
     2281 ccaggttggt gcggatcagc atcagcagcc ggccatctcc cagctcgacg acgcccggct
     2341 cggtggcgcc gtcgtggtgc ccgtgtcccc ccagatccac ccagttgctc cgctgccagt
     2401 gttggccgtc gtcgtccgac cagaaggagc agaccaccca gcgcgccggg tcgcttgcca
     2461 gatgctcgac cgtggcgacg aggcacccat cgcgcgtctg gatgaaaccg gtgaagtcgg
     2521 cgttgtaacc acccaggatg cgctgccggt cggtccaggt cgcgccgcca tccaggctgc
     2581 gaatcgacca cagctccagg cgagagtcct ccgagggctt tccggcggca tcgtcccacg
     2641 cgaacttgta gccctcaaag ttgaggaaga ggaggaccag gaccccattg cgggtgcaca
     2701 ggaactggcc gacatggccc acatgcccca ggttgacgcc tggggcgatt gggcgcgcgt
     2761 cgaaccaggt ggcgccctca tcccggctga accggatccc ctcgctgtcg atcagagcga
     2821 gcgagccgtt cggcatctgc acgaaaggcc cgttcttctc cgtggcgagt ggcttgcaga
     2881 gggggtgaac ccagcggccc tccccggcgc caatcagtcc gagcgaagcg agttgcatgg
     2941 tctgcctccc gtggagcgcc cgcgcggcac gttacttctt ctcttgtcgc gttagctggc
     3001 gcgccttttc gagcaccgcc ggaaacgcct tctccggaat ccccgagagt acccagacat
     3061 cgttgccgcg gccaagcacc gccgtcaggc attcgttgga gagccacccg cggccccagt
     3121 gatcgttcgg cagcgcgcgc aactcagtga catcctgggg ctcgaagccg attctctcgg
     3181 ccaacacctg cgcgaaggct tcacggaact cccgggcatc ctgctgagtg tcgaaggtgg
     3241 tcgcccaaac ataaagagtc tggcgcgggc cggctgtctc tgcgccggcg cg
//
LOCUS       DAOSCV010000478         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18647_length_3286_cov_4.300217, whole genome shotgun sequence.
ACCESSION   DAOSCV010000478 DAOSCV010000000
VERSION     DAOSCV010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18647_length_3286_cov_4.300217"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLY56_11840"
     CDS             complement(<1..327)
                     /locus_tag="PLY56_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22218.1"
                     /translation="MAMQNVPASAVERLAATGSEIWWDSSPLIFSAWVDEMVGKVCCP
                     NTAEALRTQLSRMYRFDDPAASVLRGITTNPPLSLAVVKGDQAGWDNWTEKYIKEHPE
                     VTTPELF"
     gene            complement(557..>3286)
                     /locus_tag="PLY56_11845"
     CDS             complement(557..>3286)
                     /locus_tag="PLY56_11845"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ22219.1"
                     /translation="TYRYIYYAGLPIFRVEADITQEKIAIWDELHFLEWHFKHDFFTR
                     FALAEPKDEEPRVVEFTNTKKSTSSRGWGALLNDRITFALSAGHQLVYDGLSDYGRYI
                     HGPWIYNWDTASRSFAATIYAGPSETTPQALAETVMPWVRGGQATASIRVPALEARLA
                     RAGAQVKPEDAAGRWLLQYAARSTRSLDSLGSALQLLAKIEARKPAPARSGAQVTIEE
                     LPDVKVATDGVLAIGFRREATGWALCSLYHVPTGHEFLDSLTTPPVIWQARLRRPDGK
                     TIDTLAGGPASITVDRAGLPRRLVLRLSWPGELSPTASITLDAGTGLSRWRFSAAKPE
                     GERALWEVDFPVLSGLGSREHPERDTAAVPRSWGQAFPNPTVTLSRHSVNYPSAGWTM
                     QYALLYHGNSGLYLGVHDPRAYSKCFTMETLHDAAGGNLAWRVTHYVPDMGKPGQGWA
                     APYDAIVGTYTGDWYEGSRIYRRFAETTPWLSKGLLAKRKDVGTAIRPLAIWMLASGG
                     PDTVVPQVKQFHEFFNVPMGVHWYNWHQIPFDDNYPHYFPTKPGFREGVAELKALGIA
                     VMPYINGRLWDTDTADWPDAKPSSAMRADGENYVEEYGSKQKLAPMCPTTPLWQQKMK
                     ETVLRLVREEGVSGVYLDQITAARPALCYNPTHGHPLGGGYHWWSGYRKLLGDIRAEL
                     ARTHPTAFLTSENNSEPWTDLLEGHLTWTPPLGDLVPMYGVVYGGYAYPFGRQMNRAA
                     LDDRNGAAIEFATQFLWGGQLAWLGNIILEPKYRPEAEYLRLLAQTRAQCIPYLAEGE
                     MLKPPAIVTPVPEITGKTIWGPHTVVDTVPAVQAVAWRAVDGSVAFFLTNLSTEAQSF
                     TWEIDFARHRLGKAAVITPLGAKEGTRVVGPRLRRKETLPGRGVLSLIARPVK"
BASE COUNT          543 a         1086 c         1084 g          573 t
ORIGIN      
        1 gaacagctcc ggcgtggtga cctcggggtg ctccttgatg tatttctcgg tccagttgtc
       61 ccagccggcc tggtcgcctt tgacgacggc gagcgagagc ggcgggttgg tggtgatccc
      121 gcgcagcacg ctggccgcgg ggtcatcgaa gcggtacatg cgcgagagct gggtgcgcag
      181 tgcctccgcg gtattcgggc agcacacctt gccaaccatc tcatcgaccc aggcggaaaa
      241 gatgaggggc gaggaatccc accagatctc gctaccggtc gccgccagac gctctacggc
      301 gctggctggc acattctgca ttgccatcag atgatgtcct ttctctgctt cactcaatca
      361 agtgcttcaa ccttcgagcg ttctacacgt cagaggaagc atccacgcgg cgaacgcccg
      421 cgaccccgga tgcccgccaa ggataccggc tgccaaacac gtatcgttgc ttcctcgttc
      481 gccgcgccgg ccctgtttcc ctttcgcaca gtgattggtc cgtggaccgg cgtggctctc
      541 ccctcgcgtt cgcggcctac ttcaccgggc gggcgatcaa agacagcacg ccgcgtcctg
      601 gcagcgtctc cttgcggcgc aggcgcgggc caacaacgcg cgtgccctcc ttggcaccca
      661 gcggcgtgat gactgcggcc ttgcccagcc ggtggcgcgc gaagtcaatc tcccaggtga
      721 agctctgcgc ctccgtcgaa aggttggtga ggaagaaagc gaccgagcca tccacggcgc
      781 gccaggcgac cgcttggacg gcaggcacgg tatccacgac cgtatggggc ccccagatcg
      841 tcttgcccgt gatttccggc acgggcgtca cgatggcggg cggcttcagc atctcgccct
      901 cggccaggta ggggatgcac tgggcgcgag tctgcgccag aaggcgcagg tactctgcct
      961 cagggcgata cttcggctcg aggatgatat tgcccagcca ggcaagctgc ccgccccaga
     1021 ggaattgggt ggcgaactcg attgccgcgc cgttgcggtc gtccagcgcg gcgcgattca
     1081 tctggcgccc gaagggatag gcgtaacccc cgtacaccac gccatacatc ggcaccagat
     1141 cgcccagagg aggagtccag gtgaggtggc cctccaggag gtcggtccac ggctcggagt
     1201 tgttctcgct ggtcaggaac gccgtggggt gcgtgcgcgc cagctccgcc cggatatcgc
     1261 ccagcagctt gcggtagcca ctccaccagt gatagccacc gccgagggga tgcccgtgcg
     1321 tcgggttata gcagagcgcg ggccgtgccg cggtgatctg gtccagatag accccggaga
     1381 cgccctcttc gcgcaccagg cgcaggaccg tctccttcat cttctgctgc cacagcggcg
     1441 tcgtcggaca catcggcgcg agcttctgct tgctcccgta ctcctccacg tagttctcgc
     1501 catcggcgcg catcgccgac gacggcttcg cgtccggcca gtcggccgtg tcggtatccc
     1561 acagccggcc gttgatgtag ggcatcaccg cgatccccag cgccttcagc tccgccacgc
     1621 cttcccggaa gccgggcttc gtcgggaaat agtgcgggta gttgtcatcg aacgggatct
     1681 ggtgccagtt gtaccagtga acgcccatcg ggacgttgaa gaactcgtgg aactgcttta
     1741 cctgaggcac gaccgtatcg ggtccgccgg aggcgagcat ccagatcgct agcgggcgga
     1801 tggcggtgcc cacgtccttc cgctttgcca gaagcccctt gctcagccag ggcgtcgtct
     1861 cggcaaagcg gcggtagatc cggcttccct cataccagtc gcccgtgtag gtgcccacga
     1921 tggcatcgta gggcgctgcc cacccctgcc ccggtttccc catatccggc acgtagtggg
     1981 tcaccctcca cgccaggttg ccacccgctg catcatggag cgtctccatg gtgaagcact
     2041 tgctgtaggc gcgcgggtca tgcaccccta ggtagaggcc ggaattgccg tgatagagga
     2101 gcgcgtactg catggtccag ccggcactcg gatagttgac agaatgccgg gagagagtca
     2161 cggtcggatt ggggaaggcc tggccccagc ttcgaggcac ggcggcagtg tcgcgctccg
     2221 ggtgctcgcg gctgcccagg ccggagagca cggggaagtc tacttcccaa agcgcccgct
     2281 ccccctcagg tttcgctgcg gagaagcgcc agcgcgagag accggttccc gcgtccaacg
     2341 tgatgctcgc cgtcgggcta agctcgccgg gccaggagag gcggaggacc aaccggcgcg
     2401 gcaggccggc gcggtccacc gtgatcgacg cgggccctcc ggcgagcgtg tcgatcgtct
     2461 ttccatccgg ccgccgcaga cgagcctgcc agatcacggg aggcgtggtc aggctgtcga
     2521 ggaactcatg cccggtggga acgtgataga ggctacaaag cgcccatccc gtcgcttccc
     2581 gacggaagcc gatcgccagc acgccatcgg tggctacctt tacatccggc agctcctcta
     2641 tcgtgacctg agcgccactc cgggctgggg ccggctttct ggcctcgatc ttggccagga
     2701 gttggagggc gctgcccagg gagtcgaggc tgcgggtgga gcgagcggca tactgcagga
     2761 gccagcgccc ggcggcatcc tctggcttca cttgggcgcc agcccgcgcc aggcgcgcct
     2821 caagcgcggg aacccggatc gatgcggtgg cctggccgcc gcgcacccag ggcataaccg
     2881 tctccgcgag tgcctgaggc gtcgtctcgg aaggccccgc gtagatcgtc gcggcaaacg
     2941 agcgggaagc ggtatcccag ttgtagatcc acggcccgtg gatgtagcgc ccgtaatcgc
     3001 tcaggccgtc gtagacgagc tggtgcccgg cggagagagc gaacgtgatg cggtcgttga
     3061 ggagggcacc ccagccgcga gaggacgtgc tcttcttcgt gttggtgaac tccaccacgc
     3121 gcggctcctc atcctttggc tcggccagcg cgaagcgggt gaagaagtcg tgcttgaaat
     3181 gccactccag gaagtggagc tcgtcccaga tcgcgatctt ctcctgggtg atatccgcct
     3241 ccacccggaa gatcgggagc ccggcatagt agatgtagcg gtaagt
//
LOCUS       DAOSCV010000479         3281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18689_length_3281_cov_3.732796, whole genome shotgun sequence.
ACCESSION   DAOSCV010000479 DAOSCV010000000
VERSION     DAOSCV010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18689_length_3281_cov_3.732796"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLY56_11850"
     CDS             complement(<1..337)
                     /locus_tag="PLY56_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22220.1"
                     /translation="MNRRAGFTLIELLVVIAIIAILAAILFPVFARARENARKANCTS
                     NVKQIIMAVLSYAQDYDETLIPGACPPKGSSYIYWYELLDPYLKNAEIIRCPSMKHAG
                     YGGLGYGWNY"
     gene            complement(696..1322)
                     /locus_tag="PLY56_11855"
     CDS             complement(696..1322)
                     /locus_tag="PLY56_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22221.1"
                     /translation="MKRRPGFTLIELLVVIAIIAILAAILFPVFARARENARKANCTS
                     NVKQVMMAILSYAQDYDEMLIPGACPPKGSSYIYWYQLLDPYLKNAAIIRCPSMKHAG
                     YGGLGYGWNYQEFGYRPDSPGTGWCTTLGQVEHPAETILLGDSEDLGARAEPQYFFYL
                     YRRHATYLPKRHLDGGIMGHVDGHVKWYATRELQKAQPGVAAPWRFAP"
     gene            1645..2232
                     /locus_tag="PLY56_11860"
     CDS             1645..2232
                     /locus_tag="PLY56_11860"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOJ22222.1"
                     /translation="MRFVTAATVALLAMAGSAAMAQQSDLPTRAGDKAIYFSIDGLGE
                     FNVGQVNVDNTFVGVSGIEGKYFFRDNMAWRLGLGFGRNETTDAQGNDVTTTRFAIAP
                     GFEYHFVTAERFSVYTGGVLSYAVLDQKTDFLGGRVVDNDQGFGLAALLGVEFYPWQN
                     VSLGAEYRAGFETGSNDTTNWAISSGGRVKLGFHF"
     gene            2653..>3281
                     /gene="radC"
                     /locus_tag="PLY56_11865"
     CDS             2653..>3281
                     /gene="radC"
                     /locus_tag="PLY56_11865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ22223.1"
                     /translation="MTTEQPHYVGHRERLRARFLQSGLEGLADYEAIELLLTLAIPRR
                     DVKPLAKTLLQRFGSIRGLLDAPSEELAGIAGVGTSVVVMIRLVRELAEVYLRQAVEG
                     REFVGSPELMARLWRARMSRLRHEVFEVAYLDSGGRLMRDGIETVEEGSIGYAAVYPR
                     RIIEAALRRGAASMVFAHNHPGGTPTPSEDDKAITRKLAQAASAVEIQVL"
BASE COUNT          619 a          999 c         1066 g          597 t
ORIGIN      
        1 ggtagttcca gccatagccc agcccgccat agccggcgtg cttcatgctg gggcaccgga
       61 taatctcggc gttcttgagg taggggtcga ggagttcgta ccagtagatg tacgagctgc
      121 ccttcggcgg gcacgccccg ggaatgagcg tctcgtcata gtcctgggcg tagcttagaa
      181 ccgccatgat gatctgcttc acgttggagg tgcagttggc cttgcgcgca ttctcgcgag
      241 cccgggcaaa gaccgggaag agaatcgccg ccagaatagc gatgatggct atgacgacga
      301 gaagttcgat aagcgtgaaa ccggcgcgcc ggttcattga aaccctcctt ccggcccggc
      361 agtgggagcg tctgatctcc cccctgctga gccggtgctc attatagcaa gcgcgtctcc
      421 ctccgtcaac gagattctca ggcctctgtt gttgctcagg gaaaatgtca ctccctacgg
      481 tgaagcgcag agtacggagt gtatcgtatt gaagagagat agcagcaccg gattggggta
      541 atcactcggt tcatggcaga gaagctagaa gcgagtgtgg ctgctgaaac cctgagcgcg
      601 gcacaggtgt cagagacgag cagcgcgggc caacaggccc gcgcccctgc ctgataaaaa
      661 ggcgttagcc tcgcgcgatg cgcgcccgcg ccaccctaag gcgcgaagcg ccacggcgcg
      721 gccacgcccg gctgggcctt ttgtagctcg cgcgtggcgt accacttcac atggccgtcg
      781 acgtggccca tgatgccgcc atccaggtgg cgcttaggca gataggtggc atgccgccgg
      841 tagaggtaga agaagtattg cggctcggca cgcgcgccca gatcctcgct atcgccgagc
      901 aaaatcgttt ccgcggggtg ctctacctgc cccagagtgg tacaccagcc agtacccggg
      961 gaatcgggcc ggtagccaaa ctcctggtag ttccagccat agcccagccc gccatagccg
     1021 gcgtgcttca tgctggggca gcgaataatc gcggcgttct tgaggtaggg gtcgaggagc
     1081 tggtaccagt agatgtacga gctgcccttc ggcggacacg cgccaggaat gagcatctca
     1141 tcataatcct gggcatagct caagatcgcc atcatgacct gcttcacgtt cgaggtgcag
     1201 ttggccttgc gcgcgttttc tcgcgcccgg gcgaagaccg ggaagaggat cgccgccaag
     1261 atggcgataa ttgcgatgac gacaaggagc tcgataagcg taaatccggg gcgccgcttc
     1321 attgtgaacc tcctttgggc tcggctgagc gagcgactca ccttccctcg actggatacc
     1381 gcgcccatta taccaagcgc tccctgtgtc gtcaacggca tcctcacccc atcgcatggg
     1441 atttggcacg tcggcatggt cgccgataga aacgggctgg tgcagggcgc acccacggag
     1501 ttgtgagccc ttgcctgaga actgagcgct tgctacctca tgttaaggtg tagtataatg
     1561 caagcgggct tgctgatcca gcaccttgtg cccaatgggg ggttcttcag gcgccggaca
     1621 gccagcccga aggagggacc ataaatgagg ttcgtaaccg cagcaacggt ggcgctcttg
     1681 gcgatggccg gcagcgccgc gatggctcag cagagcgacc tgcccacgcg ggcgggcgac
     1741 aaggcgatct acttctcgat cgatggcctc ggcgagttca acgtgggcca ggtcaatgtc
     1801 gacaacacct tcgttggtgt ctctggcatc gaggggaagt acttcttccg tgacaacatg
     1861 gcgtggcgcc tcggcctcgg tttcgggcgc aacgagacca cggatgccca gggcaacgac
     1921 gtcaccacga ctcgcttcgc catcgctcct ggctttgagt atcacttcgt caccgcagag
     1981 cgcttcagcg tctacacggg cggagtgctc tcctacgcgg tgcttgacca gaagaccgac
     2041 ttcctcgggg gccgggtggt agacaatgac cagggcttcg gactggctgc cctcctgggc
     2101 gtggagttct atccctggca gaacgtcagc ctgggcgccg agtatcgcgc gggcttcgag
     2161 accggaagca acgacaccac caactgggcc atctcgagcg gtggccgggt gaagctcggg
     2221 ttccacttct gatttcggaa gtgtgcaaga ctatcgcttg atgcggtgcg ggagggagag
     2281 aacgcgcgct ggtagcctgt gctaccatgc tccgcgagtg gcgtgatcga gcatcctcag
     2341 atgccccaca tgccccacat cctcacaaag agtggtggtg gaacactcaa ccgagagcgt
     2401 gctcctctga gtaacaccgc aacctgacta ttatccagaa cgacctagga gaggcgcagt
     2461 cgccttgtgc gcgccgccta cgggccgggt aaccttggag gccggaccaa caggcagccc
     2521 actggttgct gcgcctctgc tacgtaccaa gtggatgagt cgctgccagg agagggacgc
     2581 ggtcctgtcg aacatccccc gcatgcgagc ggcccggccc cccgggccag cggtcatggc
     2641 ggaggccccg agatgactac cgagcaaccg cactacgtgg gacacaggga acggttgcgc
     2701 gcgcgcttcc tgcagtcggg cctggaagga ttggccgact atgaggcgat cgagctgctg
     2761 ctcaccctgg ccatcccgcg ccgcgatgtg aaacccctcg ccaagacact tctccagcgc
     2821 tttggcagca tccgtggcct tctggacgcg ccttcggagg aactcgccgg gatcgccggt
     2881 gtaggcacca gtgtggtcgt gatgatccgc ctggttcggg agttggcgga ggtctacctg
     2941 cgccaggccg tggaggggcg cgagttcgtg ggcagcccgg agctgatggc gcgtctctgg
     3001 cgggcgcgca tgagccggct gcgccacgag gtgttcgagg tggcctacct ggactccggc
     3061 ggtcggctga tgcgcgacgg catcgagaca gtcgaagagg ggagcatcgg ctacgcggcc
     3121 gtctaccccc gccggatcat cgaggcggcg ctgcgtcggg gagccgcgag catggtcttc
     3181 gcgcataacc acccaggcgg cacgcccacc ccgagcgaag atgacaaggc gattacccgc
     3241 aagttggcgc aggccgcctc cgcggtcgag attcaggttc t
//
LOCUS       DAOSCV010000480         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18696_length_3280_cov_3.880000, whole genome shotgun sequence.
ACCESSION   DAOSCV010000480 DAOSCV010000000
VERSION     DAOSCV010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_3280_cov_3.880000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="PLY56_11870"
     CDS             <1..1931
                     /locus_tag="PLY56_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOJ22224.1"
                     /translation="KVTLKDRTVDRADLLRQSASRLDSNQFTLAVVGEFSRGKSSLIN
                     AILGTPELLPTSIKPATAAITVIQHGPAPGCATVHYKDGTLLEGVPLDSLRGYCTAPD
                     LDGAGLRHQISGTLARWRQQNREEVPVEEIERQAQEATPRPMVHTVTVTYPSPFLSDG
                     IVLVDTPGIGSVNPEHGEATRAFIHRADAVIFLINTDPVISASECNFLTFLQDYVSRF
                     LFVVTKIDRFDEAERRESLEYTRDIIQEFTGMPDPPIYPVSAKQYLEACASGDEAAAQ
                     ASGIPQFLSALQLFLVRERGHSFIREHVTGALTHASDLRSAIELELQGIRLSQSDLKQ
                     RLEATRPGLLGAREARDRLLEYLDSELTNVPNLLGGEADIRWIRLSASLREAIEEEIE
                     RYRWEELRQASELLPIFVRDTLTATLNDRLEEAASHLAMVRRRVIRDCRQVVDTMASR
                     IDFQIDSLRPPRELEMHLDFDPGAFVTDLKKVGTITVGSTLALSIGTAFLFGGIGALV
                     MVGGVLASTSITSMLKTRVKNQLRDALTEPLATLMDTVRVNLTEEVSRHLAEFRDDVD
                     QALTGAISNVDETLRQLEAQAAQEGFGVASREELLRSQQEALAHLDRELSLLLGPEFI
                     RRDASSGLTDLQQDQALA"
     gene            2157..>3280
                     /locus_tag="PLY56_11875"
     CDS             2157..>3280
                     /locus_tag="PLY56_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22225.1"
                     /translation="MRLIQRVGEISAGERRYARRWSAACILGGVALLLAMASAAPAGA
                     VGTTTGTGRIAVTGVHPTGYVGLLARNGLSRDILLEYQLSDPALLSHYDLIIVAGITE
                     AMLARQSPARRGAAGGSGLDALTHYVAEGGNLLIDYSGASATIISRLRGRSAQLPMGA
                     YGLPTTAQFRMIGSNNPLLGEIRQDETFPPVSLSYVPVPIDPERSVVLAEFIQPTRRI
                     PPRQARAAANRQNQQEVVQAPAGAPALLLEPLGKGKILHCGVAIGLGIGLAGVDYDRL
                     VMAMLRELTGGRATPQLTPEGTRLKRKQTSRSVGGDSADSEEEDEPAVIIPERPDGPG
                     KRGALPKGFSLVEADPATEFNLQGVLQGGQVEVLLNYWSPA"
BASE COUNT          568 a         1129 c         1059 g          524 t
ORIGIN      
        1 ccaaggtgac cttgaaagac cggaccgtag accgcgcgga tctcctgcgc cagtccgcgt
       61 ctcgcctcga cagcaatcag ttcacgctgg cggttgtggg tgagttcagc cgcggcaaga
      121 gtagcctcat caacgccatc ttgggcacgc ccgaactctt gcccacatcg atcaagccgg
      181 ccacggccgc gatcacggtc atccagcacg ggccagctcc cgggtgtgcc acggtccact
      241 ataaggatgg cactctcctg gagggcgtgc ctcttgattc cctccgcggc tactgcacag
      301 ccccagacct ggatggggcg gggctgcgcc accagatcag cggcaccctt gcgcgctggc
      361 gccagcaaaa ccgggaggag gtcccggtgg aggagatcga gcggcaggca caggaggcga
      421 ccccgcgccc gatggtacac accgtcaccg tcacctaccc ctcgccgttc ctctccgacg
      481 ggatcgtgct ggtggatacg ccgggcatcg gctcggtaaa cccagagcat ggcgaggcga
      541 cgcgcgcctt catccaccgg gcggatgccg tcatcttcct catcaacacc gacccggtta
      601 tcagcgctag cgagtgcaac ttcctcacct tcctccagga ctacgtttcg cgtttcctct
      661 tcgtggtcac caagatcgac cggtttgatg aggcggagcg ccgggaatcg ctggagtaca
      721 cccgagatat catccaggag ttcaccggga tgccggatcc cccgatctac ccggtctccg
      781 cgaagcagta tctcgaggcc tgcgcttccg gtgatgaggc agcggcgcaa gcgagcggaa
      841 tcccgcagtt cctctccgcg ctccagcttt tcctggtccg tgagcgcggg cactccttta
      901 tccgcgagca tgtcaccggc gcgctcaccc atgccagtga cctccgcagc gccatcgagc
      961 tggagctgca ggggatacgc ctctcgcaga gtgatttgaa gcaacgcctg gaggcgaccc
     1021 ggcccgggct gttgggcgcg cgcgaggctc gcgaccgcct gctggaatac ctggattccg
     1081 agctcaccaa cgtgcccaac ctcctcggtg gcgaggcgga tatccgctgg attcgccttt
     1141 ccgcctccct gcgcgaagca attgaggaag agatcgagcg gtatcggtgg gaagagctac
     1201 gtcaggcctc cgagctactc cccatcttcg tgcgtgatac cctgaccgct accctaaacg
     1261 accgtctgga ggaggcggcc tcccacctgg ccatggtccg ccgccgcgtg atccgcgatt
     1321 gccgccaggt ggtggacacc atggccagca ggatcgattt ccagattgat agcctgcgcc
     1381 cgccgcgcga actggagatg cacctcgatt tcgacccggg agccttcgtc actgacctga
     1441 agaaggtggg caccattacc gtcggcagca ccctggccct cagcattggc accgccttcc
     1501 tcttcggcgg tatcggcgct ctggtgatgg tgggcggggt cctcgcgagc accagcatca
     1561 cgagcatgct gaagacgcgc gtgaaaaacc agctccggga tgcgctgacg gaacccctcg
     1621 ccacgctgat ggacaccgtg cgcgtcaacc tgaccgagga ggtgagccgc cacctggccg
     1681 agttccgcga tgatgttgac caggcgctca ccggcgccat cagcaacgtg gatgagacat
     1741 tgcgccagct cgaggcacag gcggcgcaag aggggttcgg cgtcgccagc cgggaggagc
     1801 tgctccgcag ccagcaagag gccctggcgc atctcgaccg cgagctctcc ctgctcctgg
     1861 gccccgagtt catacgccgc gacgcctcat ccgggctcac ggatcttcaa caagatcagg
     1921 cgctggcctg aagacccgcg tgggcgggga cacccgcgag agatcacgtc gccggaccga
     1981 tgggcgcccg gggagcgggc accccgtcgc cttgttgcca gcagccagcg tacctcccgc
     2041 gcgaaacccg aacgcccttc tccgcgtcca accagcatgg gagcggcggc ccgatgggcg
     2101 cggccgcgcg aactgggcct gaagtatttc gcgccaggcc ctctgctgga ggtgagatgc
     2161 ggctcattca gagagttggg gagatctccg ctggcgagcg acgatacgcg cgccgatgga
     2221 gcgcggcctg catccttggg ggggtcgctc ttctgttggc gatggcgagc gccgcgccgg
     2281 ccggggctgt cggaaccacc acgggcaccg ggcgcatcgc ggtcacggga gtccacccga
     2341 cgggctatgt ggggctgctg gcgcgcaacg ggctatcgcg ggacattctg ctcgaatacc
     2401 agttatccga tcccgcgctc ctctcgcact atgatctgat catcgtcgcc gggatcaccg
     2461 aagccatgct ggcgcgccag tcgcccgcca ggagaggcgc cgccggcggt agcggcctcg
     2521 acgccctcac tcactacgtc gccgaaggcg gcaaccttct catcgattac agcggcgcca
     2581 gcgctacgat catctctcgg ctccggggac ggagcgcgca gctgcccatg ggggcttatg
     2641 gcctgccaac caccgcccag ttccggatga tcggcagcaa caaccccctc ctgggagaga
     2701 tccgccagga tgagacgttt ccgccagtct cgctcagcta cgtgcccgtg ccgatagacc
     2761 ccgagcgtag cgtggtgctc gccgagttca tccagcctac caggaggatt cccccgcgcc
     2821 aggcacgcgc cgccgcgaac cgacagaatc agcaggaggt ggtccaggcg cccgccgggg
     2881 cacccgccct cctgctcgag ccgctgggca aggggaagat cctccactgt ggcgtcgcca
     2941 tcggtctggg gatcggcctc gccggcgtcg attacgaccg cctggtaatg gcgatgctcc
     3001 gcgagctcac gggcggccgg gccaccccac agctcactcc cgagggcact cgcctgaaac
     3061 gcaagcagac gagccgcagc gtgggaggcg acagcgcgga ttccgaagag gaagacgagc
     3121 cggcggtgat aatcccagag cggccggacg gtccgggcaa gcggggcgcg ctcccgaagg
     3181 gcttctcgct ggtggaggcg gatcccgcca cggagttcaa cctgcagggg gtgttgcagg
     3241 gagggcaggt cgaggtgctc cttaactact ggagccccgc
//
LOCUS       DAOSCV010000481         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18752_length_3273_cov_3.108452, whole genome shotgun sequence.
ACCESSION   DAOSCV010000481 DAOSCV010000000
VERSION     DAOSCV010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18752_length_3273_cov_3.108452"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1014)
                     /locus_tag="PLY56_11880"
     CDS             complement(133..1014)
                     /locus_tag="PLY56_11880"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOJ22226.1"
                     /translation="MLALDSLPEPKRQLLRQVAAQVSQIPGVEAVVLGGSYARGMQHA
                     DSDLDVGLCYQEERPFPVADIRRVAAALSTRGTPVVTDFYEWGPWVNGGAWIHTEITK
                     VDLLYRNLDQVERTIREASEGVVQFDYLQQPPYGFPSVIYLAEIHACVPLHDPHGRVA
                     RLKEQVAVYPPKLKARLVQDFLWLAEFTLLYARGFAAVSDIYNTAGCLTRVAMCLTQA
                     LFALNERYFITDKTALAEMAAFPLAPPSYSEELARILSHPGATTGELCASVHALTALW
                     QSVVALAGGLYQPRFAL"
     gene            complement(1495..>3273)
                     /gene="leuS"
                     /locus_tag="PLY56_11885"
     CDS             complement(1495..>3273)
                     /gene="leuS"
                     /locus_tag="PLY56_11885"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOJ22227.1"
                     /translation="VQTYQEAAARKSDMDRTELAKEKTGVFTGGYAINPVNGKPIPIW
                     IADYVLISYGTGAIMAVPAHDDRDWEFARKYGLPIVEVVAGGNVEEAAYTANGVGVNS
                     ANDEVSLNGLETPEAKEKITEWLEAKGIGKRAINYRLRDWLFSRQRYWGEPFPIVFRE
                     DGTPVALPEEQLPITLPEVESYQPSGTGESPLATIQEWVNTTDPETGQPARRETNTMP
                     QWAGSCWYYLRYLDPKNDQAAWDPAKEKYWMPVDLYVGGAEHAVLHLLYARFWHKVLY
                     DAGLVSTPEPFMRLVNQGMILGFSYRYYTADGQAYSYKEVRIEGESAYVKETGALANA
                     VYVPLADVVWRDEKPYHPEYPELELEELTEKMSKSRGNVVNPDDVVREHGADALRLYE
                     MFLGPLEAVKPWNTKTVSGVSRFLHRVWRLFFDEEGTPTGTDDPASDDLTRLLHRTIK
                     KVTEDTESMNFNTAIAAMMTFVNEVTKAPARPRAILEPFVLLLAPYAPHIAEELWEAL
                     GHPETLAYEPWPAYDEALTQEQMIVLPVQVNGKVRAQVEVPADADKETVLAMAKDHER
                     VKRLLEDKILVKEIVVPGRIVNLVVK"
BASE COUNT          524 a         1055 c         1056 g          638 t
ORIGIN      
        1 cccagcccga caggacttcg ctcgcaagca gcgcggtgat ttatcgccgc gcgccgggtt
       61 gcgcgctgca tccgaggctt cggattcctt atgtcggaat ctcagtggcc tttcgccgga
      121 gccgtatttc gcctacagcg cgaaccgggg ttgatagagg ccgccggcca gcgcgaccac
      181 actctgccag agcgccgtca gtgcatgcac gctcgcgcac agttcacctg tcgtggcgcc
      241 gggatgggat aggattcggg ccagctcctc ggagtagctc gggggcgcca aggggaaagc
      301 cgccatttcc gccagcgcgg tcttgtccgt gatgaagtag cgctcgttca gggcgaagag
      361 cgcttgcgtc aagcacatcg ccactcgcgt gaggcagccg gcggtgttgt agatatcgga
      421 cacagcagca aagccgcggg catagagcag cgtgaactct gccagccaca ggaagtcctg
      481 tactagccgc gctttcagtt tgggcgggta aacagccacc tgctctttca atcgggcgac
      541 tcgcccgtgg ggatcatgca ggggcacgca ggcgtgaatc tcggccaggt agatcacgct
      601 cggaaagccg tagggcggct gctgaagata gtcgaactgg acgacgccct cgctggcctc
      661 gcggatggtt cgctccacct gatcgaggtt gcggtagagc aggtcgacct tggtgatctc
      721 ggtgtggatc caggcgcccc cgttcaccca ggggccccat tcatagaagt cggtgacgac
      781 cggcgtgcct cgggtggaga gcgcggcggc gacgcggcgg atatcggcga ccggaaacgg
      841 gcgctcctcc tggtaacaga ggcccacatc gagatcggaa tccgcgtgtt gcatcccccg
      901 ggcgtaggat ccgcccagca ccactgcctc aacacccgga atctggctta cctgcgcggc
      961 cacctgccgg agaagctggc gttttggttc cggtagagaa tcaagagcga gcaaggtgtt
     1021 cccttcaatt gcctggccgg caacagcgcc gcttcgctgc cggcctgccc tggcgtcagg
     1081 ctagcaccca cccctcactt cctatcgggg gtgctggcaa tcaacttcag gagctcttgg
     1141 ccaagtctct cgccagggtt ggccgatcgc agttgctccg ccagtttcag gagtgtctcg
     1201 cgcctcattc ctggataccg tctcgtcgcc gtagtagatc gccgcttcgg tttcgtctgt
     1261 tatccagcgc aggacgaagc gccggtaatg gtatgttttc gctttcaggc ccgagggaag
     1321 gtgctctcct gggcggggat cctattgatc ggcgaagaag gccagtgttt cctgaaccct
     1381 catttcttct atcccctggt cgctacggat gaaggaagct gcagccggga ttcctgcggt
     1441 atcataggtg ctacggcccc gctcccttgg ggagcggggc cgcgagagtg cgtgctactt
     1501 cacgaccagg ttgacgatgc ggccggggac gacgatctcc ttcaccagga tcttgtcctc
     1561 cagcagccgc ttgacgcgct cgtggtcctt ggccatagcg agcacggtct ctttatctgc
     1621 gtccgcgggt acctccacct gggcgcgcac cttgccgttc acctgcaccg gcaggacgat
     1681 catctgctcc tgagtgagcg cctcgtcgta ggccggccac ggctcgtagg cgagcgtctc
     1741 gggatggccc agcgcctccc acagctcctc ggcgatgtgc ggcgcgtagg gcgccagcag
     1801 gagcacaaat ggctccagaa tggcacgcgg gcgcgccggg gccttggtca cctcgttcac
     1861 gaacgtcatc atcgccgcga tggcggtgtt gaagttcatg ctctcggtgt cctcggtcac
     1921 cttcttgatc gtgcggtgca gcaggcgcgt caggtcatcg gacgccggat cgtcggtgcc
     1981 ggtgggcgtg ccctcctcgt cgaagaagag gcgccacaca cggtgcagga agcgcgaaac
     2041 gcccgaaacc gtcttggtgt tccacggctt caccgcctcc agggggccca ggaacatctc
     2101 gtagaggcgc agggcatcgg cgccgtgctc gcggactacg tcgtccgggt tgaccacgtt
     2161 gccgcgcgac ttgctcatct tctccgtgag ctcttccagc tccagctccg gatactccgg
     2221 gtgatacggc ttctcatcgc gccagacaac atcggccagt ggcacgtaga cggcgttcgc
     2281 gagggcgcca gtctccttca cgtaggcgct ctcgccctcg atgcgcacct ccttgtagga
     2341 gtaagcctgg ccgtccgcgg tgtagtagcg gtaggagaag cccaggatca tcccctggtt
     2401 caccaggcgc atgaacggct cgggcgtgga aaccagtccg gcatcgtaga gcaccttgtg
     2461 ccagaagcgt gcgtagagca ggtgaagcac ggcgtgctcg gccccgccca cgtagaggtc
     2521 caccggcatc cagtacttct ccttcgccgg atcccaggcg gcctggtcgt tcttgggatc
     2581 caggtagcgg aggtagtacc agcacgagcc ggcccattgg ggcatggtgt tcgtctcgcg
     2641 ccgggcaggc tggccggtct ccgggtcggt tgtgttcacc cactcctgga tcgtggccag
     2701 gggcgactcg ccggtgccgg atggttggta gctctccacc tcgggcagcg taatcgggag
     2761 ctgctcctca ggcagcgcca ctggggtgcc gtcctcgcgg aagacgatgg ggaacggctc
     2821 gccccaatag cgctggcgcg agaagagcca gtcacgcagg cggtagttga tcgccctctt
     2881 gccgatgccc ttggcctcca gccactcggt gatcttctct ttcgcctcgg gggtctcgag
     2941 gccgttcagg ctcacctcgt cgttggcgga gttgacgccg acgccattgg cggtgtaggc
     3001 ggcctcttcc acattgccac cggcgaccac ctccacgatc ggcagcccat acttgcgcgc
     3061 gaactcccag tcgcggtcgt catgcgccgg taccgccatg atcgcgccgg tgccgtagga
     3121 gatcagcacg tagtcggcga tccagatcgg gatgggcttg ccgttcaccg ggttgatcgc
     3181 gtagccgccc gtgaagacgc cggtcttctc cttcgccagc tcggtgcggt ccatatcgct
     3241 cttgcgcgcg gccgcttcct gatacgtctg cac
//
LOCUS       DAOSCV010000482         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18766_length_3270_cov_3.066874, whole genome shotgun sequence.
ACCESSION   DAOSCV010000482 DAOSCV010000000
VERSION     DAOSCV010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18766_length_3270_cov_3.066874"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PLY56_11890"
     CDS             complement(<1..225)
                     /locus_tag="PLY56_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22228.1"
                     /translation="MNLTGEIAEIAADNHSGATALAERAAALLTAAQSLPAEELPTLC
                     VALAKAQPAMAPLLRLANAVLLAAESHPASN"
     gene            complement(222..626)
                     /locus_tag="PLY56_11895"
     CDS             complement(222..626)
                     /locus_tag="PLY56_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ22229.1"
                     /translation="MRSRQGVGRFGEREAAAHLVEMGYRIREQGYRAVLGGVCIGEID
                     VIAEEGEYLVFAEVKTRSGLDRGLPREAIGRGKQRKMTRAAALYANALGEERPWRFDV
                     IEVILLPGGSTEVHVLRDAFQADERDLQGLES"
     gene            complement(616..1284)
                     /locus_tag="PLY56_11900"
     CDS             complement(616..1284)
                     /locus_tag="PLY56_11900"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ22230.1"
                     /translation="MASSADDAPFEASPEDTLRAAGHGQIAGVDEAGRGPLAGPVVAA
                     AVILPHPCPIEGINDSKRLTAAQRERVYHAILEGAVAVGIGISDVELIDRVNILQATR
                     LAMRQAVLALRPAPEFVLVDYVHIPDIPYPQRGIVHGDRLCPSIGAASIVAKVTRDRL
                     LVELDREYPQYGFAAHKGYGTPLHLARIARYGVCPAHRRSFAPVRACLEQLTLFTEET
                     DSEE"
     gene            complement(1268..2065)
                     /gene="lepB"
                     /locus_tag="PLY56_11905"
     CDS             complement(1268..2065)
                     /gene="lepB"
                     /locus_tag="PLY56_11905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ22231.1"
                     /translation="MSWESAMNDLANSWKVGIAFVVLVAIRVFLTSRAESLAHMRAAL
                     FGGIASLFGGDRPATPPNPMPSDVDSGGADAATGNGNLEAPALRSRAEEMRSSLLEFI
                     DSGLIALVLVFMIIRPFIIQAFYIPSGSMRPTLVENDKILVSKFAYRFHPLRRSDVIV
                     FKAPPEAALDEKDFIKRLIALPGDVIEVRDGMVFINGQPLHEPYIAEPPVYDMPPVTV
                     EPGKVFVMGDNRNDSNDSHAWGQLDADRIRGKALVIFWPLSRIGVIR"
     gene            complement(2333..2677)
                     /gene="rplS"
                     /locus_tag="PLY56_11910"
     CDS             complement(2333..2677)
                     /gene="rplS"
                     /locus_tag="PLY56_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ22232.1"
                     /translation="MSDLIKSIEPAVNPNIPDLHPGDTVSVHVKIREGNRERIQEFKG
                     IVLYMHGNGANSTFTVRRMASNGIGVERTFLLRSPRIDKVVVERHNVVRRAKLFYLRK
                     KIGKAARLKELR"
     gene            2996..3079
                     /locus_tag="PLY56_11915"
     tRNA            2996..3079
                     /locus_tag="PLY56_11915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3030..3032,aa:Leu,seq:taa)
BASE COUNT          641 a          975 c         1043 g          611 t
ORIGIN      
        1 gttgctggct gggtggctct ctgccgcgag gagcacggcg tttgccaggc ggaggagggg
       61 tgccatcgcc ggttgtgcct ttgcgagcgc aacacagagc gtgggcagct cctcggcagg
      121 caggctttga gcggcggtga ggagtgccgc cgcgcgttcg gcgagagcgg tggcaccgga
      181 gtggttatcc gccgcgatct cggcaatttc accggtgagg ttcatgattc cagcccctgg
      241 agatcgcgct cgtcggcctg gaacgcgtcg cgaagaacgt gcacctccgt gctgcccccg
      301 gggagaagaa tgacttcaat gacatcgaag cgccagggac gctcttcacc aagggcgttg
      361 gcatagagcg cggcagcacg cgtcatcttc cgctgcttgc cacggccaat ggcctctcgc
      421 gggagacctc ggtccagacc ggagcgcgtc tttacctccg cgaagaccaa atactcgccc
      481 tcttcggcga tcacatcgat ctcgccgatg catacgccgc cgagcacggc ccggtatccc
      541 tgttcccgga tgcgatagcc catttcgacg aggtgggcag cggcctcgcg ctcaccaaag
      601 cgcccgacgc cttgcctact cctcactatc ggtctcctcg gtgaagagag tcagctgctc
      661 taggcaggcg cgcacgggag cgaagctacg ccggtgcgcg gggcacactc catagcgagc
      721 gatcctcgct agatgcaggg gcgtgccata tcccttgtgc gcggcgaatc catactgtgg
      781 atactcgcgg tccagctcga ccagaagccg gtcgcgcgtt accttcgcga cgatggacgc
      841 ggcgccgatg gaagggcaga gccggtcgcc gtggacaatg ccgcgttggg ggtaggggat
      901 atccggaatg tgaacgtaat cgacaaggac gaactctggc gctggccgca acgcgagcac
      961 cgcctggcgc atcgccaggc gcgtcgcctg gagaatgttg acacggtcga tcagctcgac
     1021 atcagagatc ccgatgccga cggcgacggc accttcgagg atagcatggt agacgcgctc
     1081 ccgttgagcg gctgtgaggc gcttggagtc gttgattccc tcgatggggc aggggtgcgg
     1141 gagaatgacg gcggcggcga cgaccggtcc ggcaagtggc ccgcggccag cttcgtcgac
     1201 gccggcgatc tgcccatggc cggccgcccg gagtgtatcc tccgggctgg cctcaaaagg
     1261 ggcatcgtca gcggatgacg ccaatccggc taagcggcca gaagatgacc aatgctttcc
     1321 cgcggatccg gtcggcatcc agctgccccc acgcatggct atcattgctg tcattccggt
     1381 tatcgcccat gacaaacacc ttgccgggct cgaccgtgac cggaggcata tcgtagaccg
     1441 gcggttcggc gatgtagggc tcgtgcagtg gctggccatt gatgaagacc atgccgtcgc
     1501 gcacttcgat cacatcgcca gggagggcga tcaggcgctt gatgaagtcc ttctcatcca
     1561 gggccgcctc cggcggtgct ttgaagacaa tgacatcact gcggcgcagc gggtggaacc
     1621 ggtaggcgaa cttgctcacc aggatcttat cgttctccac caacgtcggg cgcatcgatc
     1681 cagaggggat gtagaaggcc tggatgataa agggccggat gatcatgaaa acgagcacca
     1741 gcgcgatcag accggaatcg atgaactcca gaagggatga gcgcatctcc tcggcccgcg
     1801 agcggagtgc cggtgcttcg agattgccgt tgcctgtggc cgcatcggcg ccaccggagt
     1861 cgacgtcaga aggcatggga ttgggaggcg tagccggccg gtcgccgccg aaaaggctgg
     1921 cgattccgcc gaagagcgcc gcgcgcatgt gcgcgagaga ttccgcccgg ctggtgagaa
     1981 acacccgaat cgcgacgaga acgacaaacg caattccgac tttccaggag ttggccaggt
     2041 cgttcattgc cgattcccag ctcaccgatt gcacctcccc ctctatccta tctcagtatc
     2101 tgctatcgca aggcactcag cgcgagggtg gcctgaaaag cccaggcgtg cgcgcgcaga
     2161 gcatcccggt tccctgcgaa tgcagatgca ggtctcgtat attatggagg acactatgac
     2221 gtgcaccgcg gtttcgcatg gcgggcggac gcgatgaaca acgcgccggg tcgcgcttcg
     2281 agtagtggca ggggagggga tgaggcggcc tcatcccctc gtaatcagcg ggtcagcgca
     2341 gctccttgag gcgcgcggcc ttgccgatct tcttgcgcag gtagaagagc ttggcgcggc
     2401 gcacaacgtt gtggcgttcg acgaccacct tgtcgatgcg gggcgagcgc agcaggaagg
     2461 tgcgctccac gccgatgccg ttggaggcca tccggcggac ggtgaaggtt gagttggcac
     2521 cgttgccatg catgtaaagc acgatgccct tgaactcctg gatacgctcg cgattgcctt
     2581 cgcggatttt cacgtgcacg ctgactgtat cgccgggatg caggtcgggg atgttcggat
     2641 tgaccgccgg ctcgatggat ttgatcaaat cgctcatcat ctcattcctt accacaatgg
     2701 gccaaaataa tagctgtgta taacattcga cgcgagggcg gattatatca cgatcatcac
     2761 ttctcgtaaa gcaacccgcg gcgacagcct gtacctcaac agcggaggga ttataccaca
     2821 ggctggggac caaacgggca ctggtgtgcg gaaatcacac gccgaatgga atacctgctt
     2881 tgtgccgtgg gggcctggag tcatccgtca gatcacgcgg ccggccccgg cgggcggaac
     2941 tcgccctccc atctcctgta atgtgcggta aaatagggtc gctataccct gatatggggc
     3001 ggtggcggaa caggcagacg cagcagactt aaaatctgcc ggccggaagg tcgtgcgggt
     3061 tcgatccccg cccgccccat ctgaggacca tgattcatgg tcttttttat taccttaccg
     3121 gtgatttgac ttgcgtttca acgatccttc gttaataata tccatgttag atcgatcgac
     3181 tcatcaattt gaaggagcag tcaacgatga acgaaaaaac cgtgatcgcc ggacatcgtc
     3241 cctttcgcgc catcaaaatc cacccgcagg
//
LOCUS       DAOSCV010000483         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18865_length_3257_cov_3.211118, whole genome shotgun sequence.
ACCESSION   DAOSCV010000483 DAOSCV010000000
VERSION     DAOSCV010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18865_length_3257_cov_3.211118"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLY56_11920"
     CDS             complement(<1..456)
                     /locus_tag="PLY56_11920"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HOJ22233.1"
                     /translation="MAETRVHHINVELPQCGNVTIPYWEVESGQEGPCFLLTAAQHGN
                     EVQGPEVIRRFIAIAGQHLKRGTILAVPFCNLPAVQRRRHHITSGPETAYRDDGGQNM
                     NRTWPGKPDGNDTERLSHAIYHAVVTRATHLLDIHSWERFGATACLPRKD"
     gene            complement(469..>3257)
                     /locus_tag="PLY56_11925"
     CDS             complement(469..>3257)
                     /locus_tag="PLY56_11925"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ22234.1"
                     /translation="PVFYTRELSVQPGSTYELSAWVKCDREFTMRYNLLGKAGAKRST
                     RLPATKGWQRVSLEAVIPENGVEAMSVGWQLLRTRGVILLDDVVIRRLNEVTVTQEAV
                     AKPHPYHRLEELASRTSFKTYALLQGADGWFQGDRVIYKDTSTGIETWMLTRSAGTST
                     RHSYMEISPWNADGSYLLFNSGQLGKGSLHMRADGSAWRYLDFYASSPIWDRRDPARV
                     YYRVYRGRDKTDLWDLAVGNVLTGAREVTRRFDGDIALWPMSQDGEKLLVREAFPDPD
                     GKMRSRLWIMNRDGKEGLMLDPGGWVHQSWFTKAPDYSIEFEWEGQTPPGQYCISTDG
                     KVRKLFDQTSGHRAHSPDGRWVAVMAGCAIRDKRTGELKAISDEPSDHQTWETDPNWY
                     CTSSGRYMRRVIAFGNETTQLLGAHNTALKHSTYWSEAHPEMSPDGTKLGYASSMMGD
                     IEFYWMVMRRPDPPQNLRARREGKRVHLSWEPGRYHKETKGYLVYRSGASGEAGELLT
                     PEPIAALTWSDTPDGLAYYRVTSVEHSGLEGLPSNEVCAFDPWEGPAAVYVEAETGHY
                     PKPAVEVFDASASGLYAVTLGKLRASPPMTLRANLPKAGEYRIWIRARGTGSLRASAG
                     GSVLGEVRCEGKAWQWLAYPTAVKLDKGEASLSLEATVVGLSVDRMLLTDAPGYRPAG
                     LGCADRQAPAKPAGLKAEAAGRYAVRLSWAPAREPDVAYYNVYAASTGDVAAVQERLV
                     GSPAEPRFVDWGLKPGTTYTYRVTAVDRAGNESPASDAVQAKTEALPARLVAGLKTTW
                     NTTLAPSVELPFTLPADGEVVVWAKVQSLDGSGRAPIRLELDGKELGRQSIPFSYISI
                     GHGGPVLKTWLWSCFRPERANPAPPMGFPVKAGGHRLTLTADPAAKVLFEEFVITNDL
                     GFVPEGTVNFRVEP"
BASE COUNT          534 a         1135 c         1015 g          573 t
ORIGIN      
        1 gtctttgcgc ggcaggcacg ccgtcgcccc gaaccgctcc cagctgtgga tatcgagcaa
       61 gtgggtcgcg cgcgtgacca cggcgtgata gatggcgtgg ctcaggcgct cggtgtcgtt
      121 cccatccggc ttgccgggcc aagtgcggtt catgttctgc ccgccatcgt cgcggtaagc
      181 cgtctccggc ccggaggtga tgtggtgccg gcgcctctga accgccggga ggttgcagaa
      241 cggcaccgcc aggatcgtcc ctcgcttcag gtgctgcccg gcaatagcga tgaaccgccg
      301 gatcacctcg ggcccctgca cctcattccc atgctgtgcc gcggtcaaga ggaagcacgg
      361 cccttcctgc ccgctctcca cctcccaata agggatcgtt acgttcccgc actgcggcag
      421 ctcaacgttg atatgatgca cgcgcgtctc tgccatccgt tttccttcct acggctccac
      481 ccggaagttg accgtgccct ccggcacaaa gcccaggtcg ttggtgatta caaactcctc
      541 gaacagcacc ttcgccgccg ggtcggcagt gagcgtcagg cggtgcccgc ccgctttcac
      601 cgggaagccc atcggcggcg ccgggttcgc gcgctctggc cggaagcagc tccagagcca
      661 tgtcttcagc accggcccac catgcccgat agagatgtag ctgaagggga tactctgccg
      721 ccccagctct tttccatcca actccaggcg gatgggcgcc ctcccgcttc catccaaact
      781 ctgcaccttc gcccacacaa ccacttcccc atcggccggc agcgtgaagg gcagctccac
      841 ggaaggcgcc aatgtggtgt tccaggtggt cttcaggccg gcgacgagcc gggcaggaag
      901 cgcctccgtc ttcgcctgca ccgcgtccga ggcaggactc tcgttgccgg cgcggtccac
      961 cgccgtcacg cggtaggtgt aggtcgtgcc aggcttcagg ccccaatcca cgaagcgtgg
     1021 ttcagcgggc gagcccacca ggcgctcctg gaccgcggcc acgtccccag tgctcgcggc
     1081 gtagacgttg taataggcca catccggctc ccgggccggc gcccaggaga ggcgcacggc
     1141 ataacgcccg gcagcttccg ccttcaaccc ggcgggcttc gccggcgcct ggcgatccgc
     1201 acatcccaaa ccggctggcc ggtagccggg cgcatcagtg aggagcatgc ggtctacgct
     1261 caggcccacc acagtggctt ctagcgagag gcttgcctcg cccttgtcca gcttcaccgc
     1321 ggtggggtaa gcgagccact gccaagcctt cccctcgcag cgcacctcgc ccaagaccga
     1381 cccgccggcc gaggccctca gagagccggt gccccgcgct cgaatccaga tacggtattc
     1441 gcccgctttc ggaaggtttg ccctcagtgt catcggcggt gaggcgcgca gcttgccgag
     1501 ggtaaccgcg tagagcccag aggcgctcgc gtcgaacacc tccaccgcgg gcttcgggta
     1561 gtgccctgtc tctgcctcca cgtagaccgc cgccggcccc tcccacgggt cgaaggcgca
     1621 gacctcgttc gaggggaggc cttccaggcc ggagtgctcc acggaggtca cgcggtaata
     1681 ggcgaggcca tccggcgtat cactccaggt gagcgcggcg attggctccg gcgtcagcag
     1741 ctcgccagcc tcgccagaag ccccggagcg gtagaccagg taacccttcg tctccttgtg
     1801 gtatcggccc ggctcccagg agagatgcac ccgtttccct tcccgacggg cgcgcaggtt
     1861 ctgaggcgga tccgggcggc gcatcaccat ccagtaaaac tcgatatccc ccatcatgga
     1921 ggaggcgtag cccagctttg tgccgtccgg gctcatctcg gggtgtgcct ccgaccagta
     1981 ggtggagtgc ttcaacgcgg tgttgtgcgc gccgaggagc tgcgtggtct cgttcccgaa
     2041 agcgatcacc cggcgcatgt agcgcccact gctcgtgcag taccagttcg gatccgtctc
     2101 ccaggtctgg tgatcggacg gctcatcact gatcgccttc agctcgcccg tgcgcttgtc
     2161 gcgaatggcg cacccggcca tgacggcgac ccagcggcca tccggactgt gcgcgcggtg
     2221 cccgctggtt tggtcgaaca gcttgcgcac cttcccgtcg gtggagatgc agtattgccc
     2281 ggggggcgtc tgcccctccc actcgaactc gatggagtaa tcaggcgcct tggtgaacca
     2341 cgactggtgg acccatcccc ccggatcgag catcaggccc tctttgccgt cccggttcat
     2401 gatccagagg cgcgagcgca tcttcccgtc cggatcggga aacgcctcgc gaacaagcag
     2461 cttctctcca tcctgactca tcggccagag ggcgatatcg ccatcgaagc ggcgggtcac
     2521 ctcgcgcgcc ccggtgagga cgttgccaac tgccaggtcc caaaggtcgg tcttatcacg
     2581 cccgcggtag acccggtagt agacgcgcgc cgggtcacgc ctatcccaga tggggctgga
     2641 ggcgtagaag tcgagatagc gccacgcgga gccatcggca cgcatgtgaa gcgagccctt
     2701 accgagttgc ccgctgttga aaagaaggta ggagccatcg gcattccagg gcgagatctc
     2761 catgtaggag tggcgcgtgc tcgttcccgc agagcgcgtc agcatccacg tctcgatgcc
     2821 ggtgctggta tccttataga tcacccggtc gccctggaac cacccatcgg ctccttgaag
     2881 gagggcatat gtcttgaagg aggtgcgcga ggccagctct tcgagccggt ggtagggatg
     2941 cggcttcgcc accgcctcct gggtgacggt gacctcgttc aaccggcgga tcacgacgtc
     3001 atccagcagg atcaccccgc gcgtgcgaag tagctgccag ccaacgctca tcgcctcgac
     3061 gccgttctcg gggatgaccg cctctagaga gacgcgctgc cagcccttcg tcgccggcaa
     3121 gcgcgtgctc cgcttggccc cggccttgcc gagcaggtta taccgcatgg tgaactcgcg
     3181 gtcacacttc acccatgccg agagctcgta agtggagcct ggctgcacgg acagctcgcg
     3241 cgtgtagaaa accggga
//
LOCUS       DAOSCV010000484         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18912_length_3252_cov_3.378793, whole genome shotgun sequence.
ACCESSION   DAOSCV010000484 DAOSCV010000000
VERSION     DAOSCV010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18912_length_3252_cov_3.378793"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLY56_11930"
     CDS             <1..703
                     /locus_tag="PLY56_11930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22235.1"
                     /translation="ITGATCVVAGIIVTLFVHEKFTPVAPEKRVGMRETFSMLLTLPA
                     LRATFGIMFITQIGLMTIQPILALFVQSLEQGSGGMLRTKVGLIFAMPGLASIVAAPL
                     WGRRGDRVGHRHTLAIALLGAGILCLPHYLVRTAAQMMVIRFSVGLCTAGILPAAHSV
                     VASAADPNRTAGALSLLSTARWLGSIAGPLMGGYLSAHFGIRPMFMITGALLLTSGLS
                     AWHAAHRTAAKANEP"
     gene            complement(755..1543)
                     /locus_tag="PLY56_11935"
     CDS             complement(755..1543)
                     /locus_tag="PLY56_11935"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015133230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOJ22236.1"
                     /translation="MAGREPLPPPLKWAGGKRWLVPRLREIYASHRERRLVEPFAGGL
                     AVALGLLPKRALLSDINPHVISFYQHLREGLVIDLPMENDASFYYAARERFNRLVAEG
                     QAHTREAAALFYYLNRTGYNGLCRFNSRGGFNVPFGRYATINYRRDFREYAHVLASWE
                     FRHGDFAGIVLREDDFVYADPPYDVQFTRYSKDDFSWDDQVRLARWLAAFDGPVVASN
                     QATPRILELYQALGFKIELLPGPRRIACDGNRAPAVEMLAARNV"
     gene            complement(1821..2615)
                     /locus_tag="PLY56_11940"
     CDS             complement(1821..2615)
                     /locus_tag="PLY56_11940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HOJ22237.1"
                     /translation="MTSVQAETPTQEWEAQDTGELFRELRSTTDPDRQEQIRDQLILM
                     HSNLVRYLARKFANRGEPLEDLIQVGTIGLINAVDRFEPDRHIKFSTYAVPTIIGEIR
                     RYFRDKGWNLKVPRRLKELNLAANRAIELLSQELGRSPTYAEIAESVGATEEEISEAL
                     EMGSAYHAVSLDTELSDDNEAARSALLDLVGETDESFRRVVLYAQLKDAINSLPERER
                     RIVEMRFFKELSQTEVAKKLGISQMHVSRLQQKALNQLREIMVRSE"
     gene            complement(2612..3028)
                     /locus_tag="PLY56_11945"
     CDS             complement(2612..3028)
                     /locus_tag="PLY56_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22238.1"
                     /translation="MEQIDLVELRIPRKPEFVSVARLAVSGIAGRMDFSYDEVEDIKL
                     AVGEACTSAMKSGSLESASSPITIRCESDPHRISIEVLDHAHPNPRGNRNVNPPADEP
                     ISRLLMEVLMDEVDVKEDEDGIVSVRMTKYVSGDEA"
     gene            complement(3028..>3252)
                     /locus_tag="PLY56_11950"
     CDS             complement(3028..>3252)
                     /locus_tag="PLY56_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ22239.1"
                     /translation="IDSVGLGVLIGGLRRARAGGGTVVLAGPNPRIRRILDITGLSSA
                     FTLAPDVDGALDALAKESSGLAQGPAQGGT"
BASE COUNT          562 a         1072 c         1014 g          604 t
ORIGIN      
        1 gatcaccgga gccacatgcg tcgtggccgg gatcatcgtc acgctcttcg tccacgagaa
       61 gttcacgccg gtggcccccg agaaacgcgt cgggatgcga gagacctttt cgatgctcct
      121 cacgctgccc gccctgcgcg cgacgtttgg aatcatgttc atcacccaga tcgggttgat
      181 gaccatccag ccgatcctgg cgctgttcgt ccagagcctg gaacagggct cgggagggat
      241 gttgcgcacc aaggtgggcc tgatcttcgc gatgcctggc ctggcatcca tcgtggcggc
      301 gccgctatgg ggccggcgtg gcgaccgggt gggccaccga cacaccctgg cgatagccct
      361 cctcggcgcc ggcatcctct gcctgccgca ctacctggtg cgcaccgcag cccagatgat
      421 ggtgatccgc ttcagcgtcg gcctctgcac ggccggcatc ctcccggcgg cacactcggt
      481 agttgccagc gccgcagacc caaaccggac ggcaggcgcc ctctcgctcc tctccacggc
      541 gcggtggctg gggagcatcg ccggacccct catgggaggc tacctctcgg cgcacttcgg
      601 gattcgcccc atgttcatga tcacgggcgc gcttctcctc actagtggcc tcagcgcctg
      661 gcatgccgca caccgcaccg cggccaaggc aaacgagccg tgagtgttct gccgccaacg
      721 ccatccccat ccgggaaggc cggccgggct gtgactacac attccgcgcc gccagcatct
      781 caacggcggg cgcgcggttt ccatcgcagg cgatccgccg tggccccggc aggagctcga
      841 tcttgaagcc gagcgcctgg tagagctcca ggatgcgcgg cgtggcctgg ttggaagcga
      901 ccacggggcc gtcgaaggcc gctagccacc tggcaaggcg tacctggtcg tcccaagaaa
      961 agtcgtcctt gctgtagcgc gtgaactgca cgtcgtacgg gggatcggca tacacgaagt
     1021 cgtcctcgcg cagcacgatc ccggcgaaat caccgtgtcg gaactcccac gaggcgagaa
     1081 cgtgcgcgta ctctcggaag tcgcgccggt agttgatggt ggcgtagcgg ccgaagggaa
     1141 cgttgaaccc acctctgctg ttgaagcggc agaggccgtt ataccccgtc cggttgagat
     1201 agtagaaaag cgctgcggcc tcccgcgtgt gggcctgccc ctcggccacc aggcggttga
     1261 accgctctcg tgccgcgtag tagaacgacg cgtcattctc catcggcaga tcgatgacca
     1321 ggccctcgcg gaggtgctgg tagaaggaga tcacgtgcgg gttgatgtcg ctgagcaggg
     1381 cacgcttggg aaggaggccc agcgccaccg ccaggccgcc tgcaaagggt tcgacgaggc
     1441 gtcgctcgcg gtgcgacgcg tatatctctc gcaggcgtgg caccaaccac cgctttcccc
     1501 ccgcccactt caggggtggg ggcagcggct ctcgccccgc catctctgaa tcctctggcc
     1561 gtctcaagcc gggcatctcc tctgcgtcat acactgagga atctcatcaa gggggttgcg
     1621 cggataggga gcgcacgggc ctggttaggc catcgtcgcg gcaacgcgca ggcagtggta
     1681 gctggtcggg ctcggtgtgc tgccgaatct cgcggcggac agggagtggc tggttatcgc
     1741 gtttgaggat gtgggcatgg ctgcggaaca cgcgccagag tcgggccggc cccgctccac
     1801 tcccacatcc ccatctgaga ctactccgag cgcaccatga tttcgcgaag ctgattgagt
     1861 gccttttgtt ggaggcgcga gacgtgcatc tgagagatgc ccagtttctt ggccacctct
     1921 gtctgggata gctccttgaa aaagcgcatc tcgacgatcc gtcgctcccg ctcgggaagt
     1981 gagttgatcg cgtccttgag ttgcgcgtag agcaccacac gccggaacga ctcgtccgtc
     2041 tcgcccacca ggtcgaggag agccgaacga gccgcctcat tgtcatcgct gagctcggta
     2101 tcgagggaga ccgcgtgata cgcggatccc atctccagag cctccgagat ctcttcctcc
     2161 gtcgccccga ccgactcggc gatctcggcg taggtgggcg agcggcccaa ctcctgcgag
     2221 aggagctcga tcgcgcggtt ggcggccagg ttcagctctt tcagtcgccg tggaacctta
     2281 aggttccagc ctttatcacg aaagtatctc cggatctcgc cgatgatggt gggtacagcg
     2341 taggtggaga acttgatgtg gcgatccggt tcgaaccgat cgacagcgtt gatcagacca
     2401 atggtgccca cctggatcaa atcttccagc ggctcgcctc gattggcgaa cttccgagcc
     2461 aggtagcgca ccaggttgct gtgcatcaag atgagctggt cacggatttg ctcttggcgg
     2521 tccggatccg tcgtggagcg cagttcacgg aacagttcgc ccgtgtcctg agcttcccac
     2581 tcctgggttg gagtctccgc ctgaacgctc gtcatgcctc atcccctgaa acgtacttcg
     2641 tcatgcgcac actgacaata ccgtcttcat cctccttcac atccacctcg tccatcagca
     2701 cttccatgag taggcggctg atcggctcat ccgcaggggg attgacgttt cgattgccgc
     2761 gtggattggg gtgcgcgtgg tccagcacct caatcgagat ccgatgcggg tcgctctcac
     2821 agcggatggt aatcggagag gacgcactct ccagagagcc gctcttcatg gcgctggtac
     2881 acgcttcacc gacggccagc ttgatatcct ccacctcgtc gtaggagaag tccatgcggc
     2941 cggcaatgcc cgagacggcc aggcgcgcga cgctcacgaa ctcgggctta cgcggtatcc
     3001 gcagctccac caaatcgatc tgctccacta ggttcctcct tgcgccggcc cctgagccag
     3061 acctgatgac tccttggcca gtgcatccag ggcaccgtcc acatccgggg caagcgtgaa
     3121 cgcgctgcta agcccggtga tgtcgaggat acgccggatc cggggattgg gcccggcgag
     3181 tacgacggta ccaccgcccg cgcgcgcccg gcgcaaccca ccgatcagca cgcccaggcc
     3241 gacgctatcg at
//
LOCUS       DAOSCV010000485         3251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18927_length_3251_cov_3.662078, whole genome shotgun sequence.
ACCESSION   DAOSCV010000485 DAOSCV010000000
VERSION     DAOSCV010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18927_length_3251_cov_3.662078"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="PLY56_11955"
     CDS             <1..1146
                     /locus_tag="PLY56_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ22240.1"
                     /translation="KGYSQALHQQLQQMGVHAMVLPIGCPYEAASLLMKGGKIDNHLP
                     EATAATVADIDGVQIAAPAFMSAIARPEEGRADVFFGIDERTFDLKNWWKILKVRKDP
                     RELQDNWETLAADPANRMSRAEFVEAMQDPNAVILGFDASLIELSSVGDEIYVPELDR
                     VFRVAGILAPTGQQDDGFFFVPLRTAQEMFQRKGEITTVQVRFTDPARSAAITEELEK
                     IPGAEVVAMGELLGTMQNLLGSARTLILSIVLVVIAITTLGVLNTVLMSVFERTREIG
                     VMRATGAEPGHVFQLIWTETLLMVGIGGALGLALAALGARAIEGAIKAALARMEMLAG
                     VKGSVIAFEPRIFVLCLLFILAVGAVAGLYPALRASRARPIEALRSE"
     gene            1186..2388
                     /locus_tag="PLY56_11960"
     CDS             1186..2388
                     /locus_tag="PLY56_11960"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ22241.1"
                     /translation="MVWFRLALKNLLRRPVRTTLTVAGIAVAIAVLYSLLEFQRGYER
                     GLREDLNALGAHIMVVPKGCPYEASTIVLHGGKWPRYLREEYLEKVRAVPGIAESAGI
                     LMDAIIAKGADERNQIFVGVDEHFRRLRQNWQFREGGWFTDDDSLIVGANVARELKLR
                     VGDTYRIREPRGNFTVDATFRVAGILEPTATQDDGFYFLPLATVQRVFNLPDRIVVIL
                     VKVKDVANITPVVDALKKSEANMNVFPLTELLSAMGQLVNNTKVFVLAIVLVAIAVGA
                     VGVLNTILMAVFERTREIGMMKAVGASAGDVFQLICAETILMCAAGGLLGTGLALAAS
                     RGTELFIKGVLAKTALISVPKGTLIGFSWPVFGAVMLLAVLLGLVAGAYPAWRASAVS
                     PVEAIRGE"
     gene            2580..>3251
                     /locus_tag="PLY56_11965"
     CDS             2580..>3251
                     /locus_tag="PLY56_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22242.1"
                     /translation="MAEKGGATARAEGLVKVYRRGAEEIRALKGIDLEIEPGEFVCLL
                     GPSGAGKTTTLNLLGLMDRPTGGRLDVAGHVVGDGARQSESELDRVRRENIGFIFQQF
                     YLMPTLSALENVEMPQLWTGKPDRARARALLERVGLGHRMAHRPGELSGGEQQRVAIA
                     RALVNRPKLLLADEPTGNLDTRTRDDILALFRELAGEGLSILLATHDQELVGYADRVV
                     QLDEGR"
BASE COUNT          545 a          963 c         1187 g          556 t
ORIGIN      
        1 aagggctaca gccaggccct ccaccagcag ctccagcaga tgggcgttca tgccatggtg
       61 ctccccatcg gctgccccta cgaggcggcc tcgctgctga tgaagggtgg caagattgat
      121 aaccacctgc cggaggcgac ggcggcgacc gtcgccgaca tcgacggcgt gcagatcgcg
      181 gcgccggcgt tcatgagcgc gattgctcgc ccggaggagg gccgcgccga tgtcttcttc
      241 ggcatcgacg agcgcacctt cgacctgaag aactggtgga agatcctgaa ggtgcgcaaa
      301 gacccgcgcg agcttcagga caactgggag accctcgcgg cggacccggc caatcggatg
      361 agccgcgccg agttcgtcga ggcgatgcag gacccgaacg cggtgatcct gggctttgat
      421 gcttcgctca tcgagctttc gagcgtgggc gatgagattt acgttccgga gctcgaccgc
      481 gtcttccgcg tggccggtat tctggcgccc acgggccagc aagatgacgg cttcttcttc
      541 gtgccgctcc ggacggcaca ggagatgttc cagcggaagg gcgagatcac cacggtgcag
      601 gttcgcttca ccgaccccgc gcgctctgcg gcgatcaccg aggagctgga gaagattccc
      661 ggcgcggaag tggtcgcgat gggcgagctg ttgggcacga tgcagaacct gctcggctcg
      721 gcgcgcacgc tcatcctctc catcgtgctg gtggtgatcg ccatcaccac gctgggcgtg
      781 ctgaacacag tgctcatgtc agtcttcgag cgcacgcgcg agatcggcgt catgcgggcc
      841 actggggcag agccgggcca cgtcttccag ctgatctgga ccgagacgct cctgatggtc
      901 gggatcggcg gcgcgctggg actggcactc gcggcgcttg gcgcccgggc gattgagggg
      961 gcgatcaagg cggcgctggc gcgcatggag atgctggccg gcgtgaaagg gagcgtcatc
     1021 gccttcgagc cgaggatctt cgtcctctgc ttgctcttca tcctggcggt gggcgcggtg
     1081 gccggcctct acccggcgct gcgcgcgtct cgcgcccggc ccatcgaggc gctccggtcg
     1141 gagtgacagg gtgatagcat cgcgcgcctt ggaaggaggc gagaggtggt ctggtttcgg
     1201 ttggcactca agaacctgct gcgacggccc gtgcgcacga cgctcacggt ggccgggatc
     1261 gcagtggcga ttgccgtact ctacagcctg ctagagttcc agaggggcta cgagcgcggc
     1321 ttgagagagg atttgaacgc tctgggcgcc catatcatgg tcgttccgaa gggctgcccc
     1381 tacgaggcga gcacaatcgt gctccacggg gggaagtggc cccgctacct gcgggaggag
     1441 tatctggaga aggtgcgcgc ggtgcccggc atcgcggaga gcgccggcat tctgatggat
     1501 gccatcatcg cgaagggtgc cgacgagaga aaccagatct tcgtgggcgt cgacgagcac
     1561 ttccgccggc tgcgccagaa ctggcagttc cgcgagggcg ggtggttcac cgacgacgac
     1621 tcgctcatcg tgggggcgaa cgtcgcccgg gagctgaagc tccgcgtggg ggacacctac
     1681 cggatccggg agccgcgcgg caacttcacc gtggatgcca ccttccgcgt ggctggcatc
     1741 ctggagccga cggcgacgca agacgacggc ttctatttcc tgcccctggc cacggtgcag
     1801 agggtgttca acctgccgga caggatcgtg gtgatcctgg tgaaggtgaa ggatgtcgcc
     1861 aacatcaccc cggtggtgga cgctctgaag aagagcgaag cgaacatgaa tgtcttcccg
     1921 ctcacggagc tgctgagcgc gatgggccag ctggtgaaca acaccaaggt gttcgtgctc
     1981 gccattgtgc tggtggcgat tgccgtgggg gctgtgggcg tgctgaacac catcctgatg
     2041 gcggtgttcg agcgcactcg cgagatcggg atgatgaagg cggtgggcgc gtccgcgggc
     2101 gacgtcttcc aactgatctg cgccgagacg atcctgatgt gcgcggctgg cgggctgctg
     2161 ggcaccgggc tggcgctggc ggcgagccgc ggcacggagc tgttcatcaa gggcgtgctg
     2221 gccaaaacgg cgctgatctc cgtacccaag ggaacgctga tcggcttctc gtggccggtt
     2281 tttggcgcgg tgatgctcct ggcggtgttg ctgggtttgg tggccggtgc ctatccggcg
     2341 tggcgggcct ccgcggtgag cccggtcgag gcgatccggg gcgagtagcc cgaagtattc
     2401 accagaatac ttcaagaaga agacgcttgc ctggtggcat gccgttgctg ccacaagcgc
     2461 ttggaagcag agtgttttcg agggcagtag ccacaaacct gggccatgcc tgggagagag
     2521 acttcttttc attattctga tgaataatgc ggggtaggcg aggaaggatg aacgaggcga
     2581 tggcggagaa aggcggggcc accgcgcgag ccgagggcct ggtgaaggtg tatcgccggg
     2641 gagccgagga gatccgggcg ctgaagggca tcgacctgga gatcgagccg ggcgagtttg
     2701 tctgccttct ggggccttcc ggcgcgggga agacgacgac gctcaacctg ctggggctga
     2761 tggaccgccc caccggaggg cgcctggatg tggctggaca cgtggtgggc gacggggcgc
     2821 gccagagtga gagcgagctc gaccgcgtcc ggcgggagaa tatcgggttc atcttccagc
     2881 agttttacct gatgcccacg ctttcggcgc tggagaacgt ggagatgccg cagctgtgga
     2941 cgggaaaacc ggaccgggcg cgcgcccggg cgctactaga gcgcgtgggc ctgggccacc
     3001 ggatggcgca ccggccagga gagctttccg gcggcgagca gcagcgcgtg gcgatcgcgc
     3061 gcgcgcttgt caaccggccg aagctgctcc tggcggacga gcccaccggc aacctcgaca
     3121 cgcgcacccg ggatgatata ctcgcgctct tccgggagct ggcgggcgag ggcctcagca
     3181 tcctcctcgc cacccatgac caggagctcg tcggctacgc agatcgcgtg gtgcagctgg
     3241 acgagggaag g
//
LOCUS       DAOSCV010000486         3250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18939_length_3250_cov_3.165258, whole genome shotgun sequence.
ACCESSION   DAOSCV010000486 DAOSCV010000000
VERSION     DAOSCV010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18939_length_3250_cov_3.165258"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(504..1019)
                     /locus_tag="PLY56_11970"
     CDS             complement(504..1019)
                     /locus_tag="PLY56_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22243.1"
                     /translation="MRSLLVCATALALSFALLGCGGGGGDSGAGGGSSSGGTSSGGSS
                     SGGMVDTQPPAIANPRLSPTTLDFRGGVVTISAEVTDASGISSVLASVQRPGTTAEQV
                     TLNLTTGTTYAGTWLAPANSSTAASATYTVSVRATDTRGNVSANHAAGTLTVLPPDSG
                     DTVPPPPVFPD"
     gene            complement(1260..1832)
                     /locus_tag="PLY56_11975"
     CDS             complement(1260..1832)
                     /locus_tag="PLY56_11975"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOJ22244.1"
                     /translation="MLKDATPIYRRATSNSPVLFRCNKDTALALVGQTKKYYAVLMID
                     RSYGFVEKSRVELHDQSVRINPDELQASHRIVQIAMEYIGVPYVWGGNTRNGLDCSGF
                     VKAVFARMGVTLPRVAREQVNVGRAVRWGELAPGDRLYFSSKGHVIDHTGIYIGGGRF
                     IHASGGAGAVVVSAITEPKYYNTLVAARRI"
     gene            complement(2222..2617)
                     /gene="rpsI"
                     /locus_tag="PLY56_11980"
     CDS             complement(2222..2617)
                     /gene="rpsI"
                     /locus_tag="PLY56_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ22245.1"
                     /translation="MAEGIRYYATGHRKNATAKVWLTPGSGNVTVNGRSLESFVGRRA
                     LEMIVRQPFEVTGSTERFDVIAKTLGGGISGQAGAIRHGIAKALVIADENLKPTLRRL
                     GFLTRDPRVKERKKYGRKRARRGFQFSKR"
     gene            complement(2723..3154)
                     /gene="rplM"
                     /locus_tag="PLY56_11985"
     CDS             complement(2723..3154)
                     /gene="rplM"
                     /locus_tag="PLY56_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ22246.1"
                     /translation="MKTYVAKPREIEREWFVIDAAGKPLGRVATRAAAILRGKHKPTF
                     TPNLDTGDHVVIINAEKAVLTGNKADEPIYRHSGYPGGLKSTTKGKVMAAHPEQLMHR
                     AVKGMLPHNKLGAQMIQKLKVYAGAEHPHAAQMPKLLEWEG"
BASE COUNT          527 a         1087 c         1047 g          589 t
ORIGIN      
        1 atgtgggacc tccagtagct cgatggggga tgcagcacgc gatgctgcat ccccttcccc
       61 gtaggaaccg ggagtgcttc gtcctgtgcg ggagaggagc gtatcccggt ttcatccccc
      121 cgcatccagg ctcccggaac gtgatcgcct gctccctctc cgcctctcca cccggccgag
      181 ccttgcgtgc ccaggcgggt ctccgggctc cgccaactcc cccatctcat cgccgcgcga
      241 cttcctgcgt cgaggcatcc ggcggcctac ccaagaagtg agtgcctcaa tggccgcacc
      301 gcctcgcttt ctggtcactg acggctgcat atgagggcag tcctcgatgc agggagcctt
      361 gggtactcgc ggatgagtgg ttggcgcggt ggcacaggag ggaaacgagt gggtgttggt
      421 acgcgcccgg ggcgcgtccc ttcaggggac gcgccccgga caaacgctcg atctggcccg
      481 cgtgagcggc actgggggca ggatcaatca gggaagacag gcggcggcgg cacggtatcc
      541 cctgagtctg gcggaaggac ggtgagcgtg ccggcagcgt ggttggcaga cacgttgccc
      601 cgggtatccg tggcacgtac cgaaaccgtg taggtggcac tggcggcggt gctggagttg
      661 gcgggcgcca gccaggtgcc ggcgtaagtc gtgcctgtcg tcaggttgag cgtcacctgc
      721 tccgccgtag tgcccggccg ctggaccgag gcgagcaccg acgagattcc ggaggcatcg
      781 gtcacctcgg cgctgatcgt caccacgccg ccccggaaat cgagggtggt aggcgagagg
      841 cgcgggttgg cgatggcagg cggctgggtg tctaccatgc ctccggagga gctgccgccg
      901 gacgaggtgc ccccggagga ggagcctcca ccagcgccac tatcgccgcc gccccctcca
      961 caaccgagca gggcgaacga gagtgctagt gccgttgcgc acacaagtaa ggatctcatt
     1021 gcttggcggg tctccttccg tcacgggcgg cgccgggagt ccgcccgcaa ccggccctat
     1081 cgcctccacc tgtcgcgtgc cggctagcag gcgggctgag tgcgctcttt ctccgcgcgc
     1141 tcgcctagcg aggccgtgct cccaggcggc aagcgcgcgg caccagccgg cgctatcgcc
     1201 gctgcttctc tcagaagcgc gcgggcacca cccacgacga gcgtgggaag cgcctggcgc
     1261 tagatgcgac gggcagcgac cagggtgttg taatactttg gctcggtgat cgcgctcact
     1321 acgacggcgc cggcaccacc cgaagcatgg ataaaccggc ctccgccgat gtagatgccg
     1381 gtgtgatcga tcacgtgccc cttcgagctg aagtaaaggc ggtctcccgg agccagctca
     1441 ccccagcgca cggcccggcc gacgttgacc tgctcgcgag ccacgcgggg cagtgtcacc
     1501 cccatgcgag cgaagaccgc cttgacgaag ccagagcagt ccagcccgtt gcgagtgttc
     1561 cctccccaca cgtagggcac gccgatgtac tccatcgcga tttgcacgat ccgatggctt
     1621 gcctgcagtt cgtccgggtt gatccggacg gattgatcat ggagttcgac ccggctcttc
     1681 tcaacgaaac cgtacgagcg gtcgatcatg agtaccgcgt agtacttctt cgtctgcccc
     1741 acaagggcga gcgcggtgtc cttattgcaa cggaagagca cggggctatt cgaggtggcc
     1801 cggcggtaga tcggggtcgc gtctttgagc acgatgccga gcctaggctc ggacttggcg
     1861 gtgctgctct gagacgcgtt cgccgctttc tgaggcgcat gcgctactag aatcgcgccc
     1921 ggcgcggctt cagtgcgctc attcgcgctc accgaccccc gtgctgcaaa cgaggcaccc
     1981 acgcccggga tgggcgtgga ggttgcctcg tttaccgatt ccagggtgat agcgatctca
     2041 ctgctcgtaa cgtgcgcgtg gatcggtgcg gcaactccga tggttgcgac cagccatccc
     2101 acaccgcatc cccaccaact ccatcgcgac attctagctt cgcttatccc catcacactt
     2161 cgatcggcgg gccccggggc gaggcccgct cgcgatccga tcctgcgccg tataaagcga
     2221 attagcgctt cgagaactgg aatccgcggc gcgcgcgctt ccgtccgtac ttcttgcgct
     2281 ccttcaccct cgggtctcgg gtgagaaagc ccaggcgccg gagcgtcggc ttcaggttct
     2341 catctgcgat gaccaacgcc ttggcgatgc cgtggcggat cgcgcccgcc tgaccagaga
     2401 taccgccgcc gagcgtcttg gcgatcacgt cgaagcgctc ggtgcttccg gtcacttcaa
     2461 acggctggcg aacaatcatc tcgagcgcgc gccggcccac aaagctctca agagagcgac
     2521 cgttcacggt cacgttcccg gaaccgggag tgagccacac cttcgcggtg gcgttcttcc
     2581 ggtgaccggt ggcatagtaa cgaattccct cagccaagga ttgtcctcct cctaatggac
     2641 gagcgaagag cgcgcttggt tcgctctatc acccttccct gtatcagcac ccgcaaaacc
     2701 ggccgtgcgg ggggcgcctg gattagcctt cccactcaag cagcttgggc atctgcgcgg
     2761 cgtgcggatg ctcggcgccc gcgtacacct tcaacttctg gatcatctgg gctcccagct
     2821 tgttgtgcgg gagcattccc ttgacggcgc ggtgcatcag ctgctcgggg tgcgcagcca
     2881 tcaccttccc cttggtggtc gacttgaggc cgccggggta gccggaatgc cggtagatcg
     2941 gctcgtcggc cttgttgcca gtcaacaccg ccttctcggc gttgataatg acgacatggt
     3001 cgccggtatc caggttcggg gtaaacgtgg gcttgtgctt cccgcgcagg atggcagcgg
     3061 cgcgcgtagc cacacggccc agcggcttgc cggcggcgtc aatcacaaac cactctcgtt
     3121 cgatctcgcg tggcttcgcc acgtaggtct tcatctacag tcctcctcaa taattcacgc
     3181 gagccaggca aagcccctgc gggggcgctg cagcgctcgc tgccgcgcgc gaccgcgcag
     3241 cgagaatgcg
//
LOCUS       DAOSCV010000487         3246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18970_length_3246_cov_3.897211, whole genome shotgun sequence.
ACCESSION   DAOSCV010000487 DAOSCV010000000
VERSION     DAOSCV010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18970_length_3246_cov_3.897211"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /gene="cas10"
                     /locus_tag="PLY56_11990"
     CDS             complement(<1..1636)
                     /gene="cas10"
                     /locus_tag="PLY56_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOJ22247.1"
                     /translation="MNAWLSFSLTPVQSFLQAARTVRDLKVGSKLLSYLAGQAFDAAC
                     AAGGRALYPAHSGDWHNLPNRFVVCYETLAQAQSAAEGVHKAFDKAWYTLADEVKKLL
                     DQQKWPAPWDEDWDKQVQTFWDARVIVVDTAAWSDDDYRALFNCASGAPLPNPLTRGW
                     KVTTTALAAAKAVRKIPPDQGIGRPKCTMMGDLEQMGPGGPVGTQRGFWRDVTGTSHH
                     GIRIGERDRLCAIALIKRFAPACDSTPLRELRCPISDTATIATEHWRKEVQEGLPDEL
                     AAFDSAAGELAKCLGDSADTSGRYLLDDNLRYNQATWSRLNEGLSPEKRHPDPRTWEQ
                     SELAGLLKKTVGARDALKRACREKGLSAPPRYLAIVELDGDHMGRLLGGDGEDVTEEH
                     FQHLSSRLRAYGDSVPGMVTEHYGETIYAGGDELLALAPRIEAIPLARELRNNFPDNL
                     AEKVTRRGSTASMGITIFHYRHDLRDALHHTHQAIDRAKKYGRDCCGIAVLKRSGSEL
                     HVVASWNVLDAMKALAESFASGVTDRWLSQLAQMAPG"
     gene            complement(1633..2853)
                     /locus_tag="PLY56_11995"
     CDS             complement(1633..2853)
                     /locus_tag="PLY56_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22248.1"
                     /translation="MPSIDVTLELVTPAYAGGASPEQCDGLRPPVLKALLRFWWRAMH
                     PELPPDELFMAEAGLFGSTGDGQRLRVIPLGDPTAMECQRPGQSLPGDTAFLSYGVSE
                     WAREARGFRTNRPRVATGQTIRFRLAWGNKIPCNAEEEFVKTLWLLSTFSGWGTKARR
                     GWGSIHVDCPCFGGAPYTNLKDPHGAASVDDLRNRLQAGLTAILGPRAGISLQPGSNE
                     AEHTAFSPDTRLFIGPGCQNAEEALDKLGQVYNRYRRALGAGRNHAPGAVGPDHQKRS
                     NWCAKPPVKTDVAPLGSAFGLPHLARFTKGPRVDVGVGAHLTGRRASPVFFKVLRCGA
                     RFVPAVLWLPARFLPVGTPVCVRAPAGESEVRDPGVTGIAQFFDGNPELMCYARGGGA
                     WLGLRGYGWTEVNW"
     gene            complement(2871..>3246)
                     /locus_tag="PLY56_12000"
     CDS             complement(2871..>3246)
                     /locus_tag="PLY56_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015174781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22249.1"
                     /translation="AGEVGLTDCARGYQTDAPHFELDVLAMRYHQLFVFSCGTTLRRD
                     DSAFRECKLKLMEARTRARQFGGDEAAVALICLLDGGCLQHLRSQVPDDDRLYVFGLP
                     DLRDLRSRLKDWILTQVAAPGA"
BASE COUNT          599 a         1085 c          966 g          596 t
ORIGIN      
        1 caccgggagc catctgcgcg agctgggaga gccaccggtc agtcacgccg ctggcaaagc
       61 tctcggcaag ggctttcatc gcatcgagaa cgttccagga tgccacgacg tgtagttcac
      121 tcccggaacg cttgaggacc gcgatgccac agcagtcccg cccatacttt tttgcacggt
      181 cgatcgcctg atgcgtatga tgcagcgcgt cacgcaggtc atgccggtag tggaagatcg
      241 tgatccccat gctcgccgtg gagccacgcc tggtgacctt ttcagcgaga ttgtcgggaa
      301 agttgttcct gagctcccgc gccagcggta tcgcctcgat ccgcggggcg agcgcaagca
      361 gctcatcgcc tccggcgtag atcgtctccc cgtagtgctc cgtcaccatc ccgggaacgc
      421 tgtcgccata tgcgcgcagc cgtgagctga gatgctggaa atgctcttcg gtcacgtctt
      481 caccatcgcc gcccaggagg cggcccatat ggtcgccatc gagctccacg atcgccaggt
      541 agcgcggcgg agccgacagc cccttttcgc ggcacgccct cttcagtgca tctcgtgcac
      601 ccacggtctt cttgaggagc cctgccagtt ccgattgctc ccacgtccgc ggatccggat
      661 ggcgtttttc cgggctcagc ccctcgttga ggcggctcca ggtcgcctgg ttgtagcgca
      721 ggttgtcgtc cagcagatac cgcccagagg tatcggcgga atcacccagg catttcgcaa
      781 gctcccctgc cgcactatcg aaagcggcaa gctcatctgg gagcccctct tgcacttcct
      841 tgcgccagtg ttccgtagcg atggtggcgg tgtcagatat cgggcaccgg agttcccgga
      901 gcggcgtgct gtcacaggca ggggcgaagc gctttatcag ggcgatggcg cagaggcgat
      961 cccgctcgcc gatccggatc ccatggtgcg acgttcccgt cacgtctctc caaaaaccgc
     1021 gctgggtacc aacggggccg ccggggccca tctgttccag gtcgcccatc atcgtgcact
     1081 tgggtcggcc aatcccctga tccggtggga tcttccgaac cgcctttgcc gcagcgagag
     1141 ctgttgtggt cactttccaa ccgcgggtga gcgggttcgg taacggggcg cccgaggcgc
     1201 agttgaacag cgcgcggtag tcgtcatcac tccaggctgc agtgtccacg acgatgacac
     1261 gtgcatccca gaacgtctgc acctgcttat cccagtcttc atcccacggg gcaggccact
     1321 tctgctgatc gaggagtttc ttcacctcat cagccagtgt gtaccacgct ttgtcgaacg
     1381 ccttgtgcac accctcggct gcgctttgcg cctgcgctag ggtttcatag cacacgacaa
     1441 agcgattggg gaggttgtgc caatcaccgg aatgtgccgg gtagagagct ctgccgcccg
     1501 cggcgcacgc ggcatcgaac gcctggcccg ccaggtagga gagaagcttc gaccccacct
     1561 tcaggtctcg cacggtacga gctgcctgaa ggaacgactg caccggcgtg agggagaaag
     1621 agagccacgc gttcaccagt tcacctccgt ccaaccataa cccctcaatc ctagccaagc
     1681 gccgccgccg cgggcatagc acatcagctc cgggtttcca tcgaagaact gggcaatccc
     1741 cgtcacaccg ggatctcgca cttctgactc gcccgcggga gcacgaacac aaacgggcgt
     1801 gccgactggg aggaagcgcg cgggaagcca caacaccgca ggtacgaatc gggcgccgca
     1861 gcgcaggact ttgaagaaga caggcgaggc acgccgtccc gtgagatgag cgccaactcc
     1921 cacatctacc cgcggacctt tggtgaatcg ggccagatgc ggcaggccaa acgccgaccc
     1981 caacggcgcg acatccgtct tgaccggggg tttagcacac cagttcgaac gtttctggtg
     2041 atcgggtccg acggcgcctg gggcatgatt gcgcccggct cccaaggcac gtcggtagcg
     2101 gttatacacc tggccgagtt tatcaagcgc ttcctctgcg ttctggcacc ccggcccgat
     2161 gaagaggcgc gtatctggcg agaaggccgt atgctctgct tcgttgcttc ccggctgcag
     2221 gcttattcca gcacgcgggc ccaggatcgc cgtcaaaccg gcctgaaggc ggttcctcag
     2281 gtcgtcgaca ctggcagctc cgtgcggatc cttcaggttg gtatagggag cgccaccaaa
     2341 gcatgggcag tccacgtgaa tgctgcccca cccgcgccgg gctttagttc cccacccact
     2401 aaaggtgctg aggagccaca gcgtcttgac gaactcctct tctgcgttgc aagggatctt
     2461 gttgccccag gccagacgaa accgaatcgt ttgccccgta gcgacgcgag ggcggttggt
     2521 tctaaatcct ctcgcttcac gggcccactc agaaacaccg taggagagga acgccgtatc
     2581 tcccggcaac gactgcccag gccgctggca ttccatcgcc gtcggatctc ccaaggggat
     2641 cactcgcagg cgctgcccgt cgccggtcga tccgaagagg cccgcctcgg ccataaacaa
     2701 ctcatccggc ggcaactcgg ggtgcatcgc ccgccaccag aagcggagca gcgccttcag
     2761 cacgggcggg cgcaggccat cacactgctc ggggctcgcc ccgcctgcgt aggcgggcgt
     2821 taccagttcc agtgtcacat cgatagaggg catggcactc ctccgttccc tcaggcgccg
     2881 ggcgcggcga cctgagtgag aatccaatcc ttgagtctgc tccgcagatc ccgcaggtca
     2941 ggaagcccga aaacgtacag gcggtcatcg tccggtacct ggctccgcag gtgctggagg
     3001 catccgccat cgagaagaca gatcaaagcg acggcggcct catcaccccc gaactgcctg
     3061 gctcgcgttc gcgcttccat tagcttgagc ttgcactccc tgaatgcact atcatctctc
     3121 ctcagcgtcg tgccacaaga gaatacaaac agttggtgat agcgcatcgc cagcacgtcc
     3181 aactcgaagt gaggcgcatc cgtttggtag ccgcgcgcgc agtcggtgag tcccacctca
     3241 ccggcc
//
LOCUS       DAOSCV010000488         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18976_length_3245_cov_3.551411, whole genome shotgun sequence.
ACCESSION   DAOSCV010000488 DAOSCV010000000
VERSION     DAOSCV010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18976_length_3245_cov_3.551411"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLY56_12005"
     CDS             complement(<1..517)
                     /locus_tag="PLY56_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ22250.1"
                     /translation="MRFALENAWLIPFFPLLGALLNGLVGKRFGKLFVSVAGVGTVGI
                     AFAHSVWAFLALREWPDRSFSRDLWSWIPMATPSRASFEAPVGFLLDPLSLVMILVVT
                     GVGLLIHVYSIGYMGHDEGYSRYFAYLNLFTASMLTLVMANNLLLMFVGWEGVGLCSY
                     LLIGFWYEKESA"
     gene            complement(630..932)
                     /gene="nuoK"
                     /locus_tag="PLY56_12010"
     CDS             complement(630..932)
                     /gene="nuoK"
                     /locus_tag="PLY56_12010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOJ22251.1"
                     /translation="MVPTVAYLMVGSILFSIGVVGVLVRRNALVVFMSIELMLNAVNL
                     TLASFARQHGHATGQVFVLFVLAVAAAEVAVGLAIIIALFRHRDTLDLDRGNLLKG"
     gene            complement(960..1454)
                     /locus_tag="PLY56_12015"
     CDS             complement(960..1454)
                     /locus_tag="PLY56_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOJ22252.1"
                     /translation="MAEWILFGIVSAIAIGSAVMMVLSRNPIHSALWLVLTFICVAVF
                     FLMVGAQFLFAVQVIVYTGAIMVLFVFVVMLLNPEREAEPLVPRAHRVASLVLSAALL
                     GVLARGIAGRAGLGSITAAPGGITLRDLGRSLYTEWLFPFELASVLLLVAMIGAIVLS
                     RRRL"
     gene            complement(1454..2038)
                     /locus_tag="PLY56_12020"
     CDS             complement(1454..2038)
                     /locus_tag="PLY56_12020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOJ22253.1"
                     /translation="MKEAIEQTLAILRGMGVTLRHIVKEPRVTIQYPEERPPQAFRYR
                     GIHQLRLDEHGKELCVGCNLCGLACPAECIYVEGAECSPEEAEHVSHYERYARVYVIN
                     AARCLYCGYCVEACPTDALAMTPQFELGEYRQEDCIYEKEDLIANYRRWMDQAGYGIE
                     WCHPEKRDEWTGHAKPLTPPSHPAAVVAEHREKR"
     gene            complement(2051..3067)
                     /gene="nuoH"
                     /locus_tag="PLY56_12025"
     CDS             complement(2051..3067)
                     /gene="nuoH"
                     /locus_tag="PLY56_12025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOJ22254.1"
                     /translation="MEYVYLAVKSVVIFAAVATAMPFVVWAERKVFADAQARIGPNRA
                     GPFGILQSFADALKLVVKEDIIPRGADKALYYLAPVLSMIPALTAFAVIPYGGPDLKI
                     AGFPIGYVTDPKEVGILYIFAITTLGVYGVVIAGWASNNKYSLLGALRCAAQMVSYEI
                     LLGLAIMGVVIASQSLSLVDIVEKQREPYWGFIPGIWLFAQPVGFLVFLIASFAEVNR
                     TPFDLPEAESELVAGFHTEYSSFKFAMFFMAEYINLITVAAVTATLFLGGWLGPGVET
                     YPILGVVYFTLKVVALLYLFMWIRATMPRLRYDQLMRFAWKFLLPVALANVFVTALWV
                     ALRS"
BASE COUNT          649 a         1125 c          980 g          491 t
ORIGIN      
        1 cagcgctctc cttctcatac cagaagccga tcagcaggta ggagcagagc ccgacgccct
       61 cccagcccac aaacatcagg agcaggttgt tcgccatcac cagcgtcagc atcgacgcgg
      121 tgaagaggtt cagataggcg aagtagcgcg agtagccctc gtcatgcccc atgtagccaa
      181 tggagtagac gtggatcagg aggccgacgc cggtgaccac caggatcatc accagggaga
      241 gcgggtcgag caagaagccg acgggcgcct caaagctcgc gcgcgacggg gtcgccatcg
      301 ggatccagga ccacaggtcg cgcgagaaac tccggtccgg ccactcgcgc agcgcgagga
      361 aggcccacac cgagtgcgcg aacgcgatcc ccaccgttcc gacgcccgcg acgctgacga
      421 acagcttccc aaagcgcttc ccgactaagc cgttcagaag cgctcctaga agcgggaaga
      481 acggtatcag ccaagcgttc tctaaagcaa agcgcatcct tcttgctccc acgcgaggcg
      541 tcgtttctgc tctcgccagg caccccaggg cagcggtcgt gcaacttgct gctgcacctt
      601 cacccagcaa gaggtccgcg cgcagcccct caccccttca gcagattccc tcgatccaga
      661 tccagggtat cgcgatgccg gaagagggcg atgatgattg ctaggcccac cgccacttcg
      721 gcggcggcca ccgccagcac gaagaggacg aagacctgtc cggtcgcgtg cccgtgctgg
      781 cgcgcgaagc tggccagggt gaggttgacg gcgttcagca tcagctcgat cgacatgaag
      841 acgacgagcg cgttccggcg cacgagcacc cccaccactc cgatactgaa gaggatcgac
      901 cccaccatca gataggccac ggttggcacc atggagcaca ccttctctct gcgcgcctgc
      961 tacagccggc gcctggatag gacaatcgcc ccgatcatcg ccaccaagag gaggaccgag
     1021 gccagctcga acgggaagag ccactcggta tagagcgagc ggcccaagtc gcgcagcgtg
     1081 atcccccccg gcgcggcggt gatcgagccg agacccgctc gcccagcgat tccccgggcg
     1141 agcaccccca ggagggcggc gctgagaacg agcgacgcca cgcggtgagc gcgcggcacc
     1201 agcggctccg cctctcgctc cgggttgagc agcatcacca cgaagacgaa gaggaccatg
     1261 atcgcgccgg tgtagacgat cacctgcacg gcgaagagga actgcgcgcc caccatcagg
     1321 aagaagaccg cgacgcagat gaaggtgagc accagccaga gcgcgctatg gatcggattg
     1381 cggctgagca ccatcatcac cgccgagccg atggcgatag cgctgacaat cccgaagagt
     1441 atccactcgg ccatcagcgt ttctcccggt gctctgccac caccgcggcc gggtgcgagg
     1501 gtggcgtgag gggctttgca tgccccgtcc actcatcccg cttctccggg tggcaccatt
     1561 cgatcccata tccggcctgg tccatccagc gccggtagtt ggcgatcagg tcctctttct
     1621 cgtagatgca gtcctcctgc cggtactccc ccagctcaaa ctgcggtgtc atcgccagcg
     1681 cgtccgtcgg gcacgcctcg acgcaatagc cgcagtagag gcagcgcgcc gcgttgatca
     1741 cgtatacacg cgcataccgc tcgtagtgcg acacatgctc cgcctcttcc ggcgagcact
     1801 cggcgccctc gacatagatg cactctgcgg ggcacgccag cccgcacagg ttgcacccca
     1861 cgcacagctc cttgccatgc tcgtccaggc ggagctgatg gatgccccgg tagcggaacg
     1921 cctgcggcgg gcgctcttcc gggtattgga tggtgacccg gggctccttc acgatgtgcc
     1981 ggagcgtcac ccccatcccc cgcagtatcg ccagcgtttg ctcgatcgcc tctttcatcc
     2041 actccccctc tcaggagcgc aacgcgaccc atagcgccgt cacgaagacg ttggccagcg
     2101 ccaccggcag caagaacttc cacgcgaagc gcatcagctg gtcgtagcgc aaccgcggca
     2161 tcgtcgcgcg tatccacatg aagaggtaga gcagcgccac caccttgagc gtgaagtaga
     2221 ccacccccag gatcgggtag gtctccacgc cggggcccag ccacccgccc aggaagaggg
     2281 tagccgtcac cgcggccacg gtgatcaggt tgatgtactc cgccatgaag aacatggcga
     2341 acttgaagct gctgtactcg gtatggaatc cggccaccag ttcgctctcc gcctccggca
     2401 ggtcgaaggg ggtccggttc acctctgcga agctcgcgat caggaagacg aggaagccca
     2461 ccggctgcgc gaagagccag atcccgggga tgaaacccca atagggctcc cgctgcttct
     2521 ccacgatatc gaccaggctg agcgattgcg aggcgatcac tacgcccatg atcgccagcc
     2581 cgagcaggat ctcgtagctc accatctgcg cggcgcagcg gagcgcgccc agcagcgaat
     2641 acttgttgtt ggacgcccat cccgcgatca ccacgccata gacgcccagc gtggtgatcg
     2701 cgaagatata gaggatcccg acctccttcg gatcggtcac gtagccgatt gggaagccag
     2761 cgatcttgag atccggaccg ccgtagggta tgacggcgaa cgccgtgagc gccgggatca
     2821 tcgacagcac cggcgctaga tagtagagcg ccttgtctgc gccgcgtggg atgatatcct
     2881 ctttgaccac cagcttcagg gcatcggcaa agctctggag gataccgaac gggcccgcgc
     2941 ggttggggcc gatgcgcgcc tgcgcgtccg cgaacacttt gcgctccgcc cagacgacga
     3001 acggcatcgc ggtcgccacc gccgcgaaga tcaccaccga tttgaccgcc aagtagacgt
     3061 attccatcta cgccagctcc agtttcacgc gatccacggc cgccgtcacg tcaagcaggc
     3121 ggttgagctc tggctccggg aagtgcagtg gcacgaagag gatccccggc gtgccctcgg
     3181 gttggacgtg cgcctgcacc tccacctccg cgcgcgcccc caccaggcgc acccgggcgc
     3241 catcg
//
LOCUS       DAOSCV010000489         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18988_length_3244_cov_3.384447, whole genome shotgun sequence.
ACCESSION   DAOSCV010000489 DAOSCV010000000
VERSION     DAOSCV010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18988_length_3244_cov_3.384447"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="PLY56_12030"
     CDS             complement(<1..2247)
                     /locus_tag="PLY56_12030"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOJ22255.1"
                     /translation="MPIHSIFVNRADDPEAVTLLARLRAQGHTRLEGLTIERVYRLEG
                     DVDPQSLMPLFVNPVFERGTPETVLDPAAGPIVEIGYQRAVTDPETPSILDGARALGV
                     DTLVWARLAHRYQFLGIDEAEARQIAEEALYNPVVQEIVPADRVWDSLRPTGTPEPVR
                     RISFAGMSEAELQRASEENSWYAPTCQMLALQKYEAEQGRPFTDAEVEIVVQSWSDHC
                     YHTTWKSLGLFQKLMDATRRINHPLVVSVFKDNAGGMLLTDDWVITIKGETHNFPSAI
                     ATFGGVATKHGGVIRDTLGFGKGATPIGGSTVMGTMDPRLPDADVPAGALHPGHIVRE
                     SIRATAYYCNPMGIPMMYPVYRSHPGYPKCLALGHSLGLVPREYATKERPQPGDAMLL
                     LGGRTGRDGLHGATASSAQMTGETAEKEAAAVQIGHPIIERKFMEAIPHLRDAGCLRT
                     ITDLGAGGISCAAGEMGEHTGVRLELDRVPLKDQSLTAWEILLSESQERMLAAVPPEK
                     LDVALEILRRFDVEATVIGHFTDTRRYEAVWRGEEVVNLEMHFLWRCCPIEPAEIRPP
                     ARLPGQESEARVGRLAPQEVTQSSLAEALEAGKAVLAHYHCCDQSPAGFQFDSTVQGR
                     TVIGPYAGVTGRMPTHAFVSAPVRGEKVGAVSTIAYNPFYGELDPGGMARLMMIEAIS
                     RAVAVGVDPEAITLCDNFYTPKATPEWNWALVQMVDTISDLSVRLGTPFISGKDSSSG
                     TFRSSDGRS"
     gene            2657..>3244
                     /locus_tag="PLY56_12035"
     CDS             2657..>3244
                     /locus_tag="PLY56_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22256.1"
                     /translation="MDERVARRRPRSRQAFLARGLPWLLALGVVLLLQHQPVYWEVAS
                     GRLRVGLQVLAMGVGFVMGWFLTEGDLTAWATWPPEGALFQRVRRRGTLALAAGWWQL
                     LWMLPAGTVVAAMVAAMVIVALIHPGEVAGTLYAADLVWRAIVAAAVSAGLSQPLRTP
                     DAKAITPRGIHTSPMTFVPWRQISHAIADPAAGVVR"
BASE COUNT          500 a         1022 c         1155 g          567 t
ORIGIN      
        1 agagcggcca tcggaagagc ggaacgtgcc ggagctggag tccttgccgg agatgaaggg
       61 cgtccccagg cgcacggaga ggtcggagat cgtgtccacc atctgcacca gcgcccagtt
      121 ccattcgggg gttgccttcg gcgtgtagaa gttgtcgcac agggtgatcg cttccggatc
      181 cacgccgacg gccaccgcgc gcgagatcgc ctcaatcatc atcaggcgcg ccattccccc
      241 tgggtctagc tccccataga agggattgta ggcgatggtg ctcaccgcgc caaccttttc
      301 gccgcgcacg ggcgccgaaa cgaaggcgtg ggtcggcatt cggcctgtga ccccggcgta
      361 gggcccgatc acggtgcgcc cctgcaccgt cgagtcgaac tggaatcctg cgggcgactg
      421 gtcgcagcag tggtaatggg caaggaccgc ttttccggct tccagcgcct ccgccaggct
      481 ggattgggtg acttcctggg gggccaaacg gcctacgcgt gcttccgact cctggccagg
      541 caggcgcgcg ggtggccgga tttccgcggg ctcgatgggg cagcaccgcc acaggaagtg
      601 catctccagg ttgacgacct cctcgccgcg ccagaccgcc tcgtagcgcc tggtatcggt
      661 gaagtgaccg atgaccgtgg cctccacatc gaagcggcgg aggatctcca gagcgacatc
      721 gagcttctcg ggcggcacgg cggcgagcat tcgctcctgc gattccgaga ggaggatctc
      781 ccaggcggtg aggctctggt cctttagagg cacgcggtcg agctcaagac gcacgccggt
      841 gtgctcgccc atctcgccgg cagcgcagga gatcccgccg gcgcccaggt ccgtgatggt
      901 gcgcagacag ccggcatcgc gcaggtgggg gatcgcctcc atgaacttcc gttcgatgat
      961 cgggtggccg atctggacgg cggcagcctc tttctcggcc gtctccccgg tcatttgtgc
     1021 cgagctggcc gtcgcgccgt gcaggccgtc gcgcccggtg cgcccgccta gcaggagcat
     1081 cgcgtcgccg ggctgggggc gttccttcgt ggcatactct cggggaacga gaccgaggga
     1141 gtggccaagg gcaaggcact tggggtagcc cggatgcgac cggtagaccg ggtacatcat
     1201 cgggatgccc atcgggttgc aatagtaggc cgtggcgcgg atcgactcgc gcacgatgtg
     1261 ccccgggtgc agcgcgccgg caggcacgtc cgcgtccggc aggcgcgggt ccatggtgcc
     1321 catcaccgtg cttccgccga tgggcgtggc tcccttgcca aacccgaggg tgtcgcggat
     1381 gacgcccccg tgcttggtgg cgacgccgcc aaaggtggcg atggcgctcg ggaagttgtg
     1441 cgtctcgcct ttgatcgtga tgacccagtc gtccgtgagg agcatgccgc cggcgttatc
     1501 cttgaagacg gagacgacga gcgggtggtt gatacgccgg gtggcatcca tcagcttctg
     1561 gaagaggccc aggctcttcc aggtggtgtg gtagcagtgg tcgctccagc tctggaccac
     1621 gatctccacc tcggcatccg tgaacggccg gccttgctcc gcctcatatt tctgaagcgc
     1681 gagcatctgg catgtgggag cgtaccacga gttctcctcc gaggcgcgct gtagctcggc
     1741 ctccgacatc cccgcgaacg agatgcggcg caccggctcc ggggtgcctg tggggcgcag
     1801 ggagtcccaa acgcgatccg cgggcacgat ctcctggacc accgggttgt agagcgcctc
     1861 ctcggcgatc tgccgggcct cggcctcgtc gatcccgagg aactgatacc ggtgggccag
     1921 gcgcgcccag acgagcgtgt ccacgcccag ggcacgggcg ccatccagaa tggatggcgt
     1981 ctctgggtcg gtaacggccc tttgatagcc gatctccacg attggcccgg ctgccggatc
     2041 gagcactgtc tccggcgtgc cgcgctcgaa gacggggttg acgaaaagcg gcatcagact
     2101 ctgggggtcc acatcgcctt caaggcggta gacccgctcg atggtgagac cctccaggcg
     2161 tgtatggcct tgggcgcgca ggcgcgcaag cagcgtgacg gcctctgggt cgtccgcgcg
     2221 gtttacaaag atggaatgga tgggcactat cagctctccc tgtcggcgcc ggggagggga
     2281 aaaggtgtcc gccaccggcg cgtatatcaa gagcggatcg cgagtggttt ctccagccag
     2341 aggagaggct cgggcgaccc atctcatttt agcatagatt ggaaggaatg ggaaccgtgc
     2401 gggcgaggga aacttgcctt gacagggcgg catgcctatg ctagaatacc tcagtatgcg
     2461 atgggccgac gggccggatc gcgccgcccg ggtccttgcg ccgggcttcc ggggacggta
     2521 ggcaggaacg ccggtccggt aggagaggtc tttccggcca caggcagcca ggagcgcctg
     2581 tggccgttgt cgtttgagcg acactctttt ccggtagagg ggcagcgggc ggaccatgga
     2641 atcactggga gcacccgtgg acgagcgcgt tgcgcgaagg cgtccacgga gcaggcaagc
     2701 ctttctggca agaggtctgc cctggctgct ggcactgggc gtcgtccttc tgcttcaaca
     2761 tcagccggta tactgggagg tcgcgagcgg gcggctgcgc gtcgggctcc aggtgctggc
     2821 gatgggggtt gggtttgtca tggggtggtt cctcaccgag ggcgacctca ccgcgtgggc
     2881 gacctggcct cccgagggcg cgcttttcca acgcgtgcgc cggcgcggca cgctcgcgct
     2941 ggcggctggc tggtggcagt tgctttggat gcttcccgcc ggcaccgtcg tagccgcgat
     3001 ggtcgcggcg atggtgatcg tggcgctcat ccacccgggg gaggtggctg gcacgctcta
     3061 cgcggcggac ctggtctggc gcgccattgt ggccgcggcg gtgagcgcgg gcttaagcca
     3121 acctctcaga acaccggatg cgaaggcgat caccccgcgc ggcattcaca cctcccccat
     3181 gacgttcgtg ccctggcggc agatctccca tgccatcgcc gatccggcgg cgggcgtggt
     3241 gcgg
//
LOCUS       DAOSCV010000490         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18997_length_3242_cov_3.252589, whole genome shotgun sequence.
ACCESSION   DAOSCV010000490 DAOSCV010000000
VERSION     DAOSCV010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18997_length_3242_cov_3.252589"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PLY56_12040"
     CDS             <1..1558
                     /locus_tag="PLY56_12040"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22257.1"
                     /translation="CLNDFDHFCRDALGRTLRPYQMAPARAVLSSVLGREGRTIAILM
                     ARQAGKNELLACLALWMLLRLPGIQIGLYAPTLPQAVDIGMRRVKRHYRRMVTKLARA
                     SGGRARRRKPGEADPPLALDLPLGVLLVPKPEVDRQDFLEFPPPEKPFGPFAGREGGS
                     MIGAHSGEKDAEKEGYTWDLILYDEAQDLDRAVIDEEISPMGSSTSATEVFIGTPYRI
                     DCKFYDVIQAIKDGTLPGECFEYSYEAVIPHVPEYGRFVEKKARELGRDSIAFRTQYG
                     LEWVHGLGLFFEFDLFRTLAREEEEFLSCMPSGAGQGTGPSPASFAVGIDLAGDDPGR
                     TGRTDYTAIAVLRRDGDAWTLVDLHQWRGKEWERQFHDISRILRSLPGRVQATIDATG
                     MGDPFSDRLQKALAGRPISIERLKLTAASKSEVGLYAEQEIAGERVFYAAGPRTRESG
                     KLDEFLRQVRWLVREHLRDKAVRWYVSEEKGHDDLVCAFFLAIWAARRSPVRRTPLAE
                     IATVDAIRYG"
     gene            1593..2213
                     /locus_tag="PLY56_12045"
     CDS             1593..2213
                     /locus_tag="PLY56_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22258.1"
                     /translation="MRFYPPLGPLGLGQRINATLHGRSRFRPADVPGHNVFNGFTRPG
                     WGDAIDLFCSGGTAVYAAEECRQVLHRHDASRLEVIYLQGSRLLLVMAHVDAARAGTG
                     HRYRAGEIVARVRSDLRDPHLHLEVACGQRGQEKPLAGRTPRELLTQLRELFLPAPAR
                     VVDVVTGDVLGEVFSLDEIPRAFPGCAVVPGGDHRADQGKVYVSRR"
     gene            2216..>3242
                     /locus_tag="PLY56_12050"
     CDS             2216..>3242
                     /locus_tag="PLY56_12050"
                     /inference="COORDINATES: protein motif:HMM:NF018886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HOJ22259.1"
                     /translation="MSLIDQVTRVFQRGRRERRTTASGLEDATSLPLGMAPEISGNGN
                     RLAAYAEVERLDAECAEASAALDTIASLISAAPEGEEIPFRVEWEGNPPAEAREAVEG
                     VIRRARLGPKTFAFARDAAKYGDNFLEVILNDALEVERLKHLPARQIYREEGAHGELL
                     DPAFTQRLYPGGTPVVSFRPWQVVHLRWGHRGDERYGRSHLAVACAIWKRLHPLEESM
                     RIARLERGYDKLVHYVEIPVEASLEEAQKRLDDYRRRMGSRVVFNPETGGYELQRQTP
                     GVRTDFYVPVSRSENGAITGGDIKLLAADNAQLAHIDDVRYLHRQLLCALRVPAAFLG
                     FEEDVNAR"
BASE COUNT          497 a         1042 c         1192 g          511 t
ORIGIN      
        1 gtgcctgaac gactttgacc acttctgccg ggatgcgctg ggccggacgc tccgcccgta
       61 ccagatggcg ccggcgcggg cggtcctgag ttcagtgttg gggcgcgagg ggcgcacgat
      121 cgccatcctc atggctcgcc aggccgggaa gaacgagctg cttgcctgtc tggcgctctg
      181 gatgctcctt cgcctgccgg gcattcagat cgggctctac gcgccgaccc tccctcaagc
      241 ggtggatatc gggatgcgtc gggtgaaacg ccactaccgg cggatggtga cgaagctggc
      301 gcgtgcgtcc ggcggcaggg cgcgccgccg caagccaggg gaggcggatc caccactcgc
      361 cctcgatctg ccgttggggg tgctcctcgt gcccaagccg gaggtggatc ggcaggattt
      421 cctcgagttt cctcccccgg agaagccgtt cggaccgttc gcggggcgcg aaggcggctc
      481 gatgatcggc gcacactcgg gcgagaagga cgccgagaag gaaggctaca cctgggacct
      541 gatcctctat gacgaggccc aggatctcga ccgcgcggtg atcgatgagg agatctcgcc
      601 gatgggctcg tccaccagcg ccacggaggt gttcatcggc acgccctacc ggatcgactg
      661 caagttctac gatgtgatcc aggcgatcaa ggacggcacg ctcccgggcg agtgcttcga
      721 gtatagctat gaagctgtga tcccgcacgt tccggaatat gggcggttcg tggagaagaa
      781 ggcgcgcgag ctcgggcgcg attcgatcgc cttccggacg cagtatggcc tggaatgggt
      841 gcatggcctc ggcctcttct ttgaattcga cctcttccgc acgctggcgc gcgaagagga
      901 ggagttcctc tcttgtatgc cctcgggcgc cggccaggga accgggccct cccccgcctc
      961 gtttgccgtg gggatcgacc tggcgggcga cgacccgggc cggaccgggc gcaccgatta
     1021 caccgcgatc gcggtgttgc gccgcgacgg cgacgcctgg acattggtgg acctgcacca
     1081 gtggcgcggt aaagagtggg agcgccagtt tcacgacatt tcgcggatcc tccggagcct
     1141 gcccgggcgc gtgcaggcga cgattgatgc gacggggatg ggcgatccct tcagcgaccg
     1201 gcttcagaag gcgctggcgg ggcgcccgat ctccatcgag cggttgaagc tgacggcggc
     1261 gagcaagtcc gaggtgggcc tctacgccga gcaggagatc gccggcgagc gcgtcttcta
     1321 cgccgccggg ccgcgcacgc gcgagtcggg caagctggat gaattcttgc gccaggtgcg
     1381 ctggctggtg cgcgagcacc tgcgcgacaa ggcggtgcgc tggtacgtga gcgaggagaa
     1441 ggggcacgac gacctggtgt gcgccttctt cctggcgatc tgggccgcgc gccggtcccc
     1501 ggtccgccgg accccgctcg ccgagatcgc gacggtggac gccatccggt atggatagcg
     1561 cctgccggcc cgcccacgga aaggagtctg tgatgcggtt ctacccgccg ctcgggccgt
     1621 tgggcctcgg acagcggatc aacgccacac tgcacgggcg cagccgcttc cggcccgcgg
     1681 atgtgcccgg gcacaacgtt ttcaacggct tcacccggcc gggatggggc gacgcaatcg
     1741 accttttctg ctcgggtggc acggcggttt acgccgccga ggagtgccgc caggtgctcc
     1801 accggcatga tgcctcgcgg ctggaggtga tctacctgca gggatcgcgc ctgctgctgg
     1861 tgatggcgca cgtggatgcc gcccgcgccg gcaccggaca tcggtatcgc gccggggaga
     1921 tcgtggcccg ggtgcgctct gacttgcgcg acccgcacct ccacctggag gtggcctgcg
     1981 ggcagcgcgg acaggagaag ccactggccg gccggacgcc cagggagctc ctgacgcaac
     2041 tgcgcgagct gttcctgccc gcgcccgcgc gcgtcgtcga cgtggtgacc ggcgatgtgc
     2101 tcggcgaggt cttctccctg gacgagattc cccgtgcctt cccggggtgc gccgtggtgc
     2161 ccgggggcga tcaccgcgcc gaccagggca aggtgtatgt gagcaggagg tagcgatgag
     2221 cctgatcgac caggtgaccc gggttttcca gcggggccgc cgggaacggc gaacgaccgc
     2281 ctcgggcctg gaggacgcga cgagcctgcc tctggggatg gcgccggaga ttagcgggaa
     2341 tggcaaccgg ctggccgcct acgcggaggt cgagcgcctg gacgcggagt gcgccgaggc
     2401 gtcggcggcg ctggacacga tcgcctcgct gatcagcgcg gcgccagagg gcgaggagat
     2461 ccccttccgc gtggagtggg aaggaaaccc gcccgccgaa gcgcgcgagg cggttgaggg
     2521 ggtgatccgg cgcgcgcgcc tgggaccgaa gacgtttgct ttcgcgcgcg acgcggcgaa
     2581 gtatggcgac aacttcctcg aagtgatcct gaacgacgcg ctggaggtag agcggctgaa
     2641 gcacctgccg gcccggcaga tctaccgcga ggagggggct cacggcgagc tgctcgatcc
     2701 ggcgttcaca caacgcctct atccgggggg cacgcccgtg gtctccttcc ggccctggca
     2761 ggtggtgcac ctgcgctggg gccatcgggg ggatgagcgg tatggccggt cacacctggc
     2821 ggtggcgtgc gcgatctgga agcgcctcca tcccctggag gaatcgatgc ggattgcccg
     2881 gctggagcgc ggctacgaca agctggtcca ctacgtcgag atcccggtgg aggcaagcct
     2941 ggaggaagcc cagaagcgcc tggacgacta tcgccggcgg atgggctcgc gcgtggtgtt
     3001 caaccccgaa accgggggct acgagcttca gcggcagacg cccggcgtgc gcaccgattt
     3061 ctacgtgccg gtgagccgca gcgagaacgg cgcgatcacc gggggcgata tcaagctcct
     3121 cgcggcagac aacgcccagc tcgcgcatat cgacgacgtg cgctacctgc accgtcagct
     3181 gctgtgcgct ctgcgcgtgc ccgcggcgtt tctggggttc gaggaggacg tgaacgcgcg
     3241 cg
//
LOCUS       DAOSCV010000491         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_18998_length_3242_cov_3.019140, whole genome shotgun sequence.
ACCESSION   DAOSCV010000491 DAOSCV010000000
VERSION     DAOSCV010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18998_length_3242_cov_3.019140"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PLY56_12055"
     CDS             <1..240
                     /locus_tag="PLY56_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22260.1"
                     /translation="NARATLAEQHAEFARTIRRGQAVLSAGYRQVFDLQLALKGFDPA
                     GLSYRIIWPDARPEEARASAEAAYYRARAERISPG"
     gene            complement(255..512)
                     /locus_tag="PLY56_12060"
     CDS             complement(255..512)
                     /locus_tag="PLY56_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22261.1"
                     /translation="MHTYLRIEELIQEGRELQGTAIAFEEREVPPNTRARYDSWKEEA
                     RRRIAGRAEPASGWDASLATGREMAREIGETLAILQRMAGA"
     gene            642..1667
                     /locus_tag="PLY56_12065"
     CDS             642..1667
                     /locus_tag="PLY56_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22262.1"
                     /translation="MKVLVEQIEPRLEVLEDAAGGERGFRIRGTALMGGGVSRNGRYY
                     PPEVVAAAAGKTPLPITLYARHRDAVEGTGLPVGRVDRLYLDGPALKYEATLAPTRLG
                     RDLRALIEGGFIAGASIRAYPYASHRARLGDRDVEWIDALTLRGIDFTDDPGVPEAAG
                     FEILEEAATGALLPNAQAATMLPEEERMNLEALDLETLRARRPDLVDAARAEDRALLE
                     ETCRVLGLAGPERLPEVVAELARLRNHREIAALADAELEGDPLRDVIKARLLEECPTL
                     ESARERLQRERTYAARLRQAVLASGLVGAGGRAPAGAGLVENPEGAAVSPDQVRIRRL
                     AGVAVEG"
     gene            1710..2939
                     /locus_tag="PLY56_12070"
     CDS             1710..2939
                     /locus_tag="PLY56_12070"
                     /inference="COORDINATES: protein motif:HMM:NF018736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22263.1"
                     /translation="MNALTEEQRRVDALVNKHAWILEGIQEERLRRSLAQLLENQEAY
                     TRGLLEDTTTADLAMPQAYSVALIRKVYPALVAYRLASIQPLRGPNGKIFYLDHVREP
                     VTTFLTAQAESGQDVIEVDSAYGLKPGDLVTIGKGDTAESRVIEAVVHKTVTLAEDLE
                     NTHPKNTPVSCAYSTAKTTEGGAVARARLSLTSMDVSAEKYALAVAWTTEAMEDLRAT
                     HNLDAEAELIDAVAGEIVREIDRAVLADMLANAGAGAVPWSATKPEGFTETEWRRTLY
                     DAIVDANNAIFKRRYRSADFLVADPDTVGRLEKLEEFHLSTEAQPSIGVQLVGRLNNR
                     WEVYKTADFPPNQILVGIKGDGYVYAPYVPLTLTPPHYDAATDTWTRALRTRAARQIT
                     VPEAYAVVTILPEAAGT"
     gene            2954..>3242
                     /locus_tag="PLY56_12075"
     CDS             2954..>3242
                     /locus_tag="PLY56_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22264.1"
                     /translation="MRPDRTQQAYYKPGAPVLHRAGVTAADAADPVSPDGAISSAGYQ
                     RVRFDLDTTGSVALAALRVRVLFWNPLAGCFFHGDDREMTGDELLASPRFSL"
BASE COUNT          500 a         1073 c         1194 g          475 t
ORIGIN      
        1 aacgcgcgcg cgacgctcgc cgagcagcac gccgagttcg cgcgaacgat ccggcgcggg
       61 caggcggttc tgtcggctgg ctaccggcag gtgttcgacc tgcagctcgc gctcaagggg
      121 ttcgatccgg cgggcctctc ctaccggatc atctggccgg atgcgcgccc ggaagaggcg
      181 cgcgcctcgg cggaggccgc ctactaccgc gcccgggcgg agaggatctc tcccgggtag
      241 agtgggctgc ctgctcaggc gccggccatc cgctggagga tcgccagcgt ttcgcctatc
      301 tcgcgcgcca tctcgcgccc ggtggcgagt gaggcgtccc atccggacgc cggctcggcg
      361 cggccggcga tcctccggcg cgcctcttcc ttccaggagt cgtagcgcgc ccgggtgttg
      421 gggggcacct cgcgctcctc gaacgcgatg gcggtgccct gcaactcgcg tccctcctgg
      481 attagctcct caatgcgcag gtaggtgtgc attcgcctct gccctccccg attgcattat
      541 agccccgggc ccagggtggg tcaagccatc gcgcggtgat ttaccgccgc gcacacgccg
      601 tggggggatg acggtttcgg atttggaaag gaggcggctt catgaaggtg ttggtcgagc
      661 agattgagcc ccggctggag gtgctggagg atgccgcggg aggcgagcgc ggcttccgca
      721 tccggggcac tgcgctgatg gggggcgggg tgtcgcgcaa cgggcgctac tacccgcccg
      781 aggtggtggc cgccgccgcg ggcaagacgc cgctcccgat cacgctctat gcccggcatc
      841 gggatgccgt cgagggcacg ggccttcccg tgggccgggt ggaccggctc tacctggacg
      901 gcccggcgtt gaagtatgag gcgacgctcg cgcccacgcg cctcgggcgc gacctgcgcg
      961 cgctgatcga gggcggcttc atcgccggcg cgagcattcg tgcttacccc tatgccagcc
     1021 atcgcgcgcg cctgggcgac cgggatgtgg agtggatcga cgcgcttact ttgaggggga
     1081 tcgacttcac cgacgatccg ggcgtgcccg aggccgcggg cttcgagatc ctggaagagg
     1141 ccgccacggg cgcgctactt ccgaatgccc aggcggccac gatgctgccc gaggaggaga
     1201 ggatgaacct ggaagcgttg gacctggaga cgttgcgcgc gcgacgcccc gacctggtgg
     1261 acgcggcgcg cgcggaggat cgggccctcc tggaggagac ctgccgcgtg ctgggtctgg
     1321 ccgggccgga acggctgccg gaggtagtgg ccgagctcgc gcggttgcgc aaccatcgcg
     1381 agattgccgc cctggccgac gcggagctcg agggcgaccc gcttcgcgac gtgatcaagg
     1441 cgcgcctgct ggaggagtgc cccacgttgg agtcggcgcg agagcgccta cagcgcgagc
     1501 gcacctacgc ggcccggctg cgccaggcgg tgcttgcctc gggccttgtg ggcgccggcg
     1561 ggcgcgcgcc ggcgggcgcc gggctggtgg aaaacccgga gggcgccgcg gtctcgccgg
     1621 atcaggtgcg aatccgccgg ctggccgggg tggcggtgga ggggtgaccg cctggcacgg
     1681 cgggtgacac gcacggaaag gagctacgaa tgaacgcgct cactgaagag caacggcgcg
     1741 tggacgcgct ggtgaacaag cacgcctgga tcctggaagg aattcaggag gagcgcctgc
     1801 gccggtcgct cgcccagctg ctggagaacc aggaggccta cacgcgcggc ctgctggagg
     1861 atacgaccac ggcggatctc gcgatgccgc aggcctactc cgtggcgctc atccggaagg
     1921 tctatcccgc gctcgtggcc taccggctgg cgagcatcca gccgctgcgc ggcccgaacg
     1981 ggaagatctt ctacctggat cacgtgaggg agccggtgac cactttcctc accgcgcagg
     2041 cggagagcgg ccaggacgtg atcgaggtgg acagcgccta cggcttgaag ccgggcgacc
     2101 tcgtgacaat cggcaagggt gacaccgccg aaagccgggt gatcgaggcg gtggtccata
     2161 agacggtgac tctcgcggaa gatctggaaa acacgcatcc caagaacacg ccggtgagct
     2221 gcgcctactc cacggccaag accaccgagg gcggcgccgt ggcccgtgcc cggctctcgc
     2281 tcaccagcat ggatgtttca gccgagaagt acgcgctggc ggtggcgtgg accacggagg
     2341 cgatggagga cctgcgcgcg acgcacaacc tggatgccga agccgagttg atcgacgcgg
     2401 tggcgggcga gatcgtgcgc gagatcgacc gggccgtgct cgccgacatg cttgccaacg
     2461 ccggcgccgg cgccgtgccc tggagtgcca ccaagcctga gggcttcacc gagacggagt
     2521 ggcggcgcac gctctacgac gcgatcgtgg acgcgaacaa cgccatcttc aagcgccgct
     2581 accggagcgc cgacttcctg gtggccgatc ccgatacggt gggccggctg gagaagctgg
     2641 aagagttcca tctgagcacg gaggcccagc cgtccatcgg cgtgcagctg gtgggccggc
     2701 tcaacaaccg ctgggaagtg tataagacgg cggactttcc cccgaatcag atcctggtgg
     2761 ggatcaaggg cgatggctac gtctacgcgc cctacgtgcc cctcaccctg acgcctcccc
     2821 actacgacgc cgccacggac acctggacgc gcgctctgcg cacgcgggct gcccggcaaa
     2881 tcacggtgcc ggaggcctac gccgtcgtca cgatccttcc cgaagcggcg ggcacgtgag
     2941 aggagcgagg cgcatgcggc cagacagaac gcaacaggcc tactacaagc cgggcgctcc
     3001 tgtgctccat cgcgcgggag tgacagccgc ggatgccgcg gacccggtct ctcccgatgg
     3061 ggcgatctcg agcgcgggtt accagcgggt gcggtttgac ctggacacca ccggctcggt
     3121 ggcgctggcg gcgctgcggg tgcgggtgct tttctggaac ccgctggccg ggtgcttctt
     3181 ccacggcgac gaccgcgaga tgaccggaga cgagctcctg gccagcccga ggttctcgct
     3241 cg
//
LOCUS       DAOSCV010000492         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19004_length_3241_cov_3.792216, whole genome shotgun sequence.
ACCESSION   DAOSCV010000492 DAOSCV010000000
VERSION     DAOSCV010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19004_length_3241_cov_3.792216"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLY56_12080"
     CDS             <1..1131
                     /locus_tag="PLY56_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ22265.1"
                     /translation="GLVAVSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGRMDI
                     DLATEPLGHDPQGQPVYLRDIWPTQQEITEAIARSVRSEMFAAEYGEVFEGSEMWRAL
                     PAPEGDRFAWNDASTYVKHPPYFTDLPAEPVPVADLRGARVLAFLGDSVTTDHISPAG
                     SIKKESPAGRYLMELGVPPAEFNSYGSRRGNHEVMMRGTFANIRLRNKLVPGVEGGIT
                     LHLPTGEQMSIYDAAMRYAAESTPLIILAGKEYGSGSSRDWAAKGPRLLGVRAVIAES
                     YERIHRSNLVGMGILPLQYRPGENAETLGLTGREVFDIEGLGEAVASGFAGVRELRVR
                     AVSPDGGSVEFTAIARIDTPQECLYYQHGGILAYVLRQLLKE"
     gene            complement(1226..1552)
                     /locus_tag="PLY56_12085"
     CDS             complement(1226..1552)
                     /locus_tag="PLY56_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22266.1"
                     /translation="MNAMWRLNTREAIAYVENRDTALELICSLKATSKARALDEAMAV
                     YVTPRGRAFAWQIRFPYDRWSSVARTLEDAEDGRLRIERELPDEDDARAAEEPAQTAR
                     ARKPRR"
     gene            2064..>3241
                     /locus_tag="PLY56_12090"
     CDS             2064..>3241
                     /locus_tag="PLY56_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOJ22267.1"
                     /translation="MKVDPSIFKAYDIRGIVPEQLDEEIAYKIGRAFVRVVNAQQVVV
                     GRDMRVSGPMLFDALARGITDEGAHVLDIGLSATDIYYYACATTASPGVMITASHNPK
                     EYNGFKMVREMPYLLSGDAGIQDIRRLVEADEFGAPAKTRGQIRTMDVREGFVAKVLS
                     LIDVEALRPLKVVIDAGNGMGGVMAEAVYQHLPVELVRLYFEPDGTFPNHGGDPLLPE
                     NRRELEKRVVAEGAHCGFAFDGDADRFFSVDDRGKFVPGDFMTALLAREFLADHPGAK
                     IIYDLRASHAVPNMVREAGGVPLMNRVGHAFIKKRMKDEDAIFAGEVTGHYYFRDFFY
                     ADSGIIPSLKVMEMLSRHQCKLSALLEPLEAKYFISGEINVPVENAAAKLQRLKERYA
                     "
BASE COUNT          570 a         1072 c         1040 g          559 t
ORIGIN      
        1 ggcctggtgg cagtctccgt gctgagcggc aaccgcaact tcgaggggcg catcagtccg
       61 gacgtgcgcg ccaactacct ggcctcgccg ccgctcgtcg tcgcctacgc ccttgccggg
      121 cgcatggata tcgacctcgc aaccgagcca ttgggccatg acccccaggg ccagccggtc
      181 tatctgcgcg acatctggcc cacccagcag gagatcaccg aggcgatcgc gcgctcggtg
      241 cgctccgaga tgttcgcggc ggagtatggg gaggtgttcg agggctctga gatgtggcgc
      301 gcgctccctg cgccggaggg cgaccgcttt gcctggaacg atgcatccac ctacgtcaag
      361 cacccgccct acttcaccga tctgcctgcc gaaccggtgc ccgtggccga cctgcgtggc
      421 gcacgcgtcc tcgccttcct cggcgatagc gtgaccacgg accacatctc tccggccggc
      481 tcaatcaaga aggagagccc ggcggggcgc tacctgatgg agctgggcgt gcctccggca
      541 gagttcaact cctacggctc tcgccggggc aaccacgagg tgatgatgcg cggcaccttc
      601 gccaatatcc gcctgcgcaa taagctggtg cccggcgtcg agggaggcat caccctccac
      661 ctgcctacag gcgagcagat gtcgatctac gatgccgcga tgcgctacgc cgcggagagc
      721 acgccgctca ttattctggc cggcaaggag tacggctcgg gatcctctcg cgactgggcg
      781 gcgaaaggcc cgcgcctgct aggcgtgcgc gccgtgatcg ccgagagcta cgagcgcatc
      841 caccgcagca acctggtcgg catggggatt ctcccgctcc agtaccgccc cggagagaac
      901 gccgagacgc tcgggctgac cgggcgcgag gtcttcgata tcgagggtct gggcgaagcc
      961 gtggccagtg gtttcgctgg cgtgcgtgag ttgcgcgtcc gcgccgtctc gcccgacggc
     1021 gggagcgtgg agttcaccgc cattgcccgg atcgataccc cccaggagtg cctctactac
     1081 cagcatggcg gcatcctcgc ctacgtgctc cggcagcttc tgaaggagta actacgtcgc
     1141 ggcccccgtg gcggtaccgc cacaggggcc gcgcccgtcc catccactcc gaacgcgtgc
     1201 gtgatgccag ccggcttccg cgcattcacc ggcgtggctt gcgtgcgcgg gcggtctgtg
     1261 ccggctcctc cgcggctcgg gcgtcgtcct cgtccggcag ctctcgctca atgcgcagcc
     1321 ggccatcctc cgcgtcctcc agagtgcgcg ccaccgagct ccaacggtcg tacggaaaac
     1381 gaatctgcca ggcaaacgcc cggccacgcg gagtcacata gaccgccatc gcctcgtcca
     1441 gggcacgcgc cttggatgtc gccttcagcg agcagatcaa ctccaaggcg gtgtcgcggt
     1501 tctccacgta agcaatcgcc tctcgcgtgt tcaggcgcca catcgcattc acaagcgtga
     1561 cttcccctca agatagcatc tggttgaaca gtccgattat agcaaacgca tgtatgcctg
     1621 tcaacctcag taccggaaga tcgccccatc ggggtcctcc gccggtgcat cccgtgtggc
     1681 ccaattacca agccgaaggg gtggcacaac acagcccatc ggtgatacca cggatgaaag
     1741 ggccccacac ggatgggccg cgcgggcagc aggagcaact gcctaaggca ggcactcgcg
     1801 gatagccggc ggctctcgcc aacgaggcaa aacgacatca tcggtgtccg ccccctcatc
     1861 cgtggtatcg ctgccggcgt ggggcagaaa accccgcgtt tgacctgggg caggagtgcg
     1921 ccttcggggg tgcgaactga tattgatgtg agcgtgtggc atgtcgccgt gggaaccccg
     1981 ccttgtgtca cagcggatgc gccagtgcgc aggcaggagt agcctgctgc actcgcagta
     2041 tcgccataca ggagggacaa agagtgaagg tagatccgag catcttcaag gcctacgata
     2101 tccggggcat cgtgcccgag cagttggacg aggagatcgc ctacaagatc gggcgggcgt
     2161 tcgtgcgtgt cgtcaacgcc cagcaggtgg tcgtaggaag agacatgcgt gtctccggcc
     2221 cgatgctctt tgatgccctc gcgcgcggga tcaccgacga gggcgcccat gtgctggaca
     2281 ttggcctctc cgccacggac atctactact atgcgtgcgc gacgactgct tccccgggcg
     2341 tcatgatcac ggcatcgcac aacccaaagg agtacaacgg cttcaagatg gtccgagaga
     2401 tgccctacct cctgagcggc gacgcgggca tccaggatat ccgccggttg gttgaagccg
     2461 acgagtttgg cgcgcccgcg aaaacacgcg ggcagatccg gaccatggac gtgcgcgagg
     2521 gcttcgtcgc caaggtgctt tcgctcatcg atgtggaggc gttgcgcccc ctcaaggtcg
     2581 tcatcgatgc cggcaacggc atgggcgggg tgatggccga agcggtctac cagcatctgc
     2641 cggtggaact ggtccgcctc tacttcgagc cggatggcac cttccccaac cacggcggcg
     2701 atcccttact cccggagaac cggcgcgagc tggaaaagcg cgtggtggca gagggcgcgc
     2761 attgcggttt cgccttcgat ggcgatgccg accgcttctt ctccgtagac gaccgcggga
     2821 agttcgttcc gggcgatttc atgacggcgc ttctggcgcg cgagttcctg gcggaccatc
     2881 cgggcgccaa gatcatctac gacctccggg cttcgcatgc cgtgcccaac atggtccggg
     2941 aggccggcgg agtgcccctc atgaaccgtg tcgggcacgc cttcatcaag aagcggatga
     3001 aggacgagga tgccatcttc gccggtgagg tgaccgggca ctactacttc cgcgatttct
     3061 tctatgccga ctctggaatc atcccctcgc tgaaggtgat ggagatgctc tcgcggcacc
     3121 agtgcaagct ctcggcgctg ctggagcccc tggaagcgaa gtacttcatc tcgggcgaga
     3181 tcaacgtgcc ggtagagaac gccgccgcga agctgcagcg gttgaaggag cgctatgccg
     3241 a
//
LOCUS       DAOSCV010000493         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19005_length_3241_cov_3.328311, whole genome shotgun sequence.
ACCESSION   DAOSCV010000493 DAOSCV010000000
VERSION     DAOSCV010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19005_length_3241_cov_3.328311"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..2764)
                     /locus_tag="PLY56_12095"
     CDS             complement(275..2764)
                     /locus_tag="PLY56_12095"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ22268.1"
                     /translation="MKRVAWILVAGILWMLSPAGGYGQDVGEAPVDAPEDTRNYAGPG
                     LGARFSPGVNTEFLEPWGLDAFVTKGTLIFRHGPRDLEKAIIVTLAQPVRVRAVSFGT
                     VYEDIVRCPIGCEVFASETGIENSYRRVGGTNQVRGDRTLIPIEPPVVARYLKFAFTD
                     SGGGSRITGLGVYTGTPERVRQAGGFEPWPPGPQPDWITPDAITAAAREGEPRGDMEL
                     TFYATGLDYSAPAIPGEGSLLERFHREGGTVARRLERLPHDLPLYRALGATAVESYVR
                     WNFIEERQGVFDFSVHDAVLAQLREHGLKWVPLVIAGPAYSLPAWFRESPDFLGFRCL
                     EHGQENGVASIWNPRLRPYVERFLQAFFEHYAPHADSIQSVILGISGIFGENLYPHDA
                     SRDWTTNAFGDYHTHSGWWAGDAHAQASFRAYLKARYKTVAALNAAWGTTWESFDAIT
                     PQAPETLSEGRARYDFLDWYNSSMTEYLEFWLRTARKHTRGKLLICVGGHGLPRVGAD
                     FSATARLAARYGAGIRITNEGPDYRWNFAMTRWISTAARFYRAPLGIEPASLQVDARS
                     IAARVFNARTSGAEELFCYPSSWTNKPGYLKLAEHLHYLRRDTPVTSVAFRVPRTHLM
                     AVGEVDYLGQMAVLREATDFDAVDAALIRDDALKSYQLLILGQGNVEEAAMLERICRW
                     VYQGGILVRLGRAPLRTVDQRDDYERWFLQNGGQEARLPSGAVSRRVGGGYVVDVRDV
                     PESAEAFAALMDQVLVDATRISRRFVRPPRLVGAPRGVYVAATRGDLLFLNTTGNQVD
                     AEYEAYAPGGIVRRGSISLPPQAMRSVAYPR"
     gene            complement(2928..>3241)
                     /locus_tag="PLY56_12100"
     CDS             complement(2928..>3241)
                     /locus_tag="PLY56_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOJ22269.1"
                     /translation="GKAVGRTNGWPRLADVAGKPDPKIMEVSRYFDCVNFATRAKAEA
                     IFSVGFIDGTCAPTSVYAAYNAWPGKKQIINEPLMGHSVSRKLGEVTAARIRAQIDAR
                     K"
BASE COUNT          546 a         1102 c         1062 g          531 t
ORIGIN      
        1 tcacactctc cgcggatcac ctcttcgtgc ccgccccggc gcaagggaag tgatccggcc
       61 ccggtttcag ggacccaatc gtctcgcgtc tcagcccggc atcgcctgcc gggctgagcg
      121 cgctatcccg cattattcat cagaataatg aaaagaagtc tctctgccag gcgtggcact
      181 ggttcgtggg cactgtcctc gggcgcctgt ggcggcaacg gtgtgtcacc agacaagggt
      241 cttctcttga aagtattctg gtgaatactt cgggctatcg gggatacgcc acggagcgca
      301 tcgcctgcgg gggaagcgag atgctcccgc gccggacgat ccctccgggg gcatacgcct
      361 cgtactccgc gtccacctgg ttcccggtgg tgttcaagaa gagcaggtca ccacgtgtgg
      421 cggcgacata gacgccgcgc ggcgcgccca cgaggcgcgg cgggcgcacg aaccgccggc
      481 tgatccgggt cgcgtcaacg agcacctgat ccatgagcgc cgcgaacgcc tctgccgatt
      541 cgggcacgtc gcgcacatcc acgacgtagc cgccccccac gcgccggctg accgctccgg
      601 aagggaggcg agcctcctgg ccgccgttct ggaggaacca ccgctcataa tcatcgcgct
      661 ggtccactgt gcgcagtggc gcccgcccga gacgcaccag aatgcccccc tggtagaccc
      721 accggcagat ccgctccagc atcgccgctt cctcgacatt cccctgcccg agaatcagga
      781 gctggtacga cttcagcgcg tcatcgcgga tcagggcggc gtccacagca tcgaaatcgg
      841 tcgcctcgcg aagcacagcc atctgcccca ggtaatccac ctccccaacg gccatcaggt
      901 gcgtgcgcgg cacccggaac gcgacggagg tgacgggggt atcgcggcgg agatagtgaa
      961 ggtgctcggc cagtttcagg tagcccggct tgttcgtcca gctcgacgga tagcagaaga
     1021 gctcttccgc gccggaggtt cgcgcgttga agacgcgtgc cgcgatggag cgcgcatcca
     1081 cctgcaatga agccggctcg atacccaagg gagcgcggta gaaacgagcc gcggtgctga
     1141 tccagcgcgt catcgcgaag ttccaccggt aatcggggcc ctcgttggtg atccggatcc
     1201 cggcgccata gcgcgcggcg aggcgtgccg ttgcagagaa gtcggcgcct acgcgcggca
     1261 acccgtgccc accgacgcag atgagcagct tgccccgcgt gtgtttgcgc gccgtgcgca
     1321 gccagaactc cagatactcg gtcatggagc tgttgtacca gtcgaggaaa tcgtagcgcg
     1381 cgcgcccctc gctcaaggtc tccggcgctt gcggcgtgat cgcgtcaaac gattcccacg
     1441 tggttcccca ggcggcgttg agcgcagcga ccgtcttata gcgcgccttg aggtaggcgc
     1501 ggaagctcgc ctgcgcgtgg gcgtcgccgg cccaccaccc ggagtgggtg tgatagtcgc
     1561 cgaaggcgtt ggtggtccag tcgcggctcg cgtcgtgggg atagaggttc tcgccgaaga
     1621 tcccgctgat ccccaggatc acgctctgga tcgaatcggc gtggggcgca tagtgctcga
     1681 agaacgcctg gaggaagcgc tctacgtagg ggcgcaggcg agggttccag atcgaggcga
     1741 cgccattctc ctggccatgc tccaggcagc ggaagccgag gaaatcgggg gactcgcgaa
     1801 accatgccgg aagcgagtag gccggcccgg caatgaccag aggtacccac ttcaggccgt
     1861 gttcgcgcag ctgcgccagc accgcgtcgt ggaccgagaa atcgaaaacg ccctgccgct
     1921 cctcgatgaa gttccagcgc acgtagctct cgaccgcggt ggctcccaga gcgcgataga
     1981 gaggcaggtc gtggggaagc cgctccagcc ggcgggcaac cgttcccccc tcgcggtgga
     2041 agcgctccag aagcgagccc tcgcctggga tggcgggcgc ggagtagtca aggccggtgg
     2101 cgtagaaggt gagctccata tcgccgcggg gctcgccctc acgcgcagcc gcggtgatgg
     2161 catccggcgt gatccaatcc ggctggggtc cgggcggcca cggctcgaac ccgcccgcct
     2221 gacgcacgcg ctccggagtg ccggtataga cgcccagccc cgtgatccgc gagccaccgc
     2281 cggaatcggt gaaagcgaac ttcaggtagc gcgccactac aggcggctcg atcggaatga
     2341 gcgtgcggtc gccgcgcacc tggttcgtcc cgcccacccg gcggtaggag ttctcgatgc
     2401 cggtttccga ggcgaagacc tcgcacccga tggggcagcg gacgatatcc tcgtagacgg
     2461 tgccgaagct cacggcgcgc acgcgcactg gctgcgcgag cgtgacgatg atcgccttct
     2521 ccaggtcacg cgggccgtgc cggaagatga gcgtgccctt cgtcacgaac gcatccaggc
     2581 cccatggctc caggaactcc gtgttcacgc ccgggctaaa gcgcgcgccc aggcccgggc
     2641 cggcgtagtt ccgggtgtcc tcgggcgcat cgacaggggc ttcccccaca tcctggccgt
     2701 agccacctgc tggtgagagc atccacagga tgccggctac caggatccaa gctaccctct
     2761 tcatgctcct gccgctcctg ctcactttgc cacgcggcat tccccgcgca tgaagaatcg
     2821 ttcgccatcc ggcgcgcgca gccctgttgt gcccctcgcc cgcattattc tggtgaataa
     2881 ttcgggctag atgcggggag cgagtcgggc ccgctccccg cgtccattca cttgcgcgcg
     2941 tcgatctgcg cccggatccg cgccgcggtc acctcgccca gcttccggga gacgctatgg
     3001 cccatcaggg gctcgttgat gatctgcttc ttcccgggcc aggcgttgta ggccgcgtag
     3061 acgctcgtcg gcgcgcacgt gccgtcgatg aagcccacgc taaaaatcgc ctcggctttg
     3121 gcgcgcgtcg cgaagttgac gcagtcgaag tagcgcgaga cttccatgat cttcggatcg
     3181 ggcttgcccg ccacatcggc cagacggggc caaccgttcg tgcgtccgac ggccttgccg
     3241 g
//
LOCUS       DAOSCV010000494         3238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19034_length_3238_cov_3.018850, whole genome shotgun sequence.
ACCESSION   DAOSCV010000494 DAOSCV010000000
VERSION     DAOSCV010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19034_length_3238_cov_3.018850"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PLY56_12105"
     CDS             complement(<1..696)
                     /locus_tag="PLY56_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ22270.1"
                     /translation="MDAPVSATRSMARGALAMMVSQGVTWSATLVLTLYMPRALGAAG
                     YGRLQFVLSLISIFALFIELGATTLITREVARDRSRAPAYLIHALLLQAVTALAAYAC
                     LIGAVLLLRRPPEVVQLAWVLGVGMILFAATAFLNSFLRGLEQVHYQAWGSVLEKVLE
                     AAIILALLSAGGGLIPIAWAMVGVGVIHLAWTAYWVWRLVHPRWSAPRAEFLKEMVRE
                     SLPFFLFALFAQIY"
     gene            complement(681..1589)
                     /locus_tag="PLY56_12110"
     CDS             complement(681..1589)
                     /locus_tag="PLY56_12110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22271.1"
                     /translation="MVSIIIPAHNVAEFIGDAICSALAQSYPRIEVIVVDDGSTDDTP
                     EVVRRFGREVRLIRQENAGPSAARNAGLAAARGTHVAFLDGDDAFDPTRIEVLLDALR
                     DASPAAAFATTDARLWDGTRFLRRFAISTPVPGERDLSTFLDETWCYIGILARRDTLL
                     AAGGFRQELRHCEDYDLWLRLLAGGKTYAYVPRPLYHYRVRESSLSNDTRQQIGAALR
                     VEREALRTLPLSWGQRRQLAFLAWEDRARLHAMDAERARAEGRGLARGGHLAARGACH
                     GIRAALRPGVCLSRLAHRGGAAWMHR"
     gene            complement(1583..2293)
                     /locus_tag="PLY56_12115"
     CDS             complement(1583..2293)
                     /locus_tag="PLY56_12115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ22272.1"
                     /translation="MYHRIGEGERDPLLRRFTLSRQRFEAQMRCLHRLRFHAVSPERI
                     AQWLDGRAPLPPRPVAITFDDGYADTYHIAAPILEAYRFRATLFLVSDYIGRKATWDA
                     DVAADDGFPLMSWEEARDLARRGHWIGSHTRTHRELPLLDEGALAEELLGSRLDLQRQ
                     LGQRVHILAYPYSQVDDRCRRAARAAGYRAACAGEGLDYHRWCLDRVNTAEASLPLFL
                     AKLSPWFYLFRRARAGRW"
     gene            complement(2344..>3238)
                     /locus_tag="PLY56_12120"
     CDS             complement(2344..>3238)
                     /locus_tag="PLY56_12120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22273.1"
                     /translation="SLRVLRFAADNDSDYCDALSDPEYPQDLGALWTALRECDDWHVL
                     DLRYVPEQGNVTRLLPARDTGILLVRQRNDIAPYMDLTEDWRKSLSKQRRDNILRRTR
                     RLREKGVLSFEVAAHPESVDEMLAQMAKIHVARWQARGQTSMFCFEEYRAWLQAFCQR
                     LLRARQLYLCRLALNGDPVSIGLYFLDGRRILGYISTFAASYADYSPGHILEAMVIDD
                     VRARGLAEIDDFGRGGEEYKLQWAREVTVLDRFLVARKTLAGVPAFWWATQIQPFLWR
                     NHHLGTLAREARRRMALRGRR"
BASE COUNT          575 a         1093 c         1114 g          456 t
ORIGIN      
        1 gtagatctgc gcgaagaggg cgaagagaaa gaaggggagc gactcgcgca ccatctcctt
       61 caggaactcc gcgcgcggcg ccgaccagcg cgggtgcacc agccgccaca cccagtaggc
      121 ggtccacgcg aggtggatca cgcccacgcc aaccatcgcc caagcaatcg ggatcagccc
      181 gcctcccgcc gagagaagcg ccaggatgat cgcggcctcc agcacctttt ccagcacgga
      241 tccccaggcc tggtagtgta cctgctcgag gccgcgcaga aagctgttga ggaacgccgt
      301 cgccgcgaag agaatcatcc ccacgccgag aacccaggcg agctggacca cctcgggtgg
      361 ccggcgcagg agcagcaccg cgccaatgag acaggcatac gcggccaggg cggtcaccgc
      421 ttgcagcagg agcgcgtgga tcagataggc gggagcgcgc gagcggtcgc gcgccacctc
      481 gcgcgtgatc agcgtcgtgg cccccagctc gatgaagagc gcgaagatgc tgataagaga
      541 gagcacgaac tggagccggc cgtatccagc ggcgcccaga gcccggggca tgtagagcgt
      601 gagcaccagc gtggccgacc aggtgacgcc ctgcgagacc atcatcgcga gggcgccgcg
      661 cgccatcgag cgcgtcgcgc tcaccggtgc atccatgccg cacctccccg atgcgccaga
      721 cgagagaggc ataccccggg ccgcagtgcc gcccggatgc cgtggcacgc cccgcgcgcc
      781 gccaggtgac ctccccgggc caaccctctt ccctcggcgc gtgccctctc tgcgtccatc
      841 gcatggagtc gtgcccgatc ctcccaggcc aggaaggcaa gctggcgtcg ctggccccac
      901 gaaagcggga gagtgcgcag ggcctcgcgc tccacccgga gcgccgcgcc aatctgctgg
      961 cgggtatcgt tcgagaggct gctctcgcgc acgcgatagt gatagagcgg ccttggaaca
     1021 taggcatacg tcttcccgcc ggcgagcagg cgcagccaga ggtcataatc ctcgcagtgc
     1081 cgcagctcct ggcggaaccc gccggccgcg aggagcgtgt cgcgacgggc cagtatcccg
     1141 atgtagcacc acgtctcatc gaggaaagtg ctcaggtcgc gttctcctgg aacgggcgtg
     1201 gagatcgcga aacgccggag gaagcgcgtt ccgtcccata gccgggcatc agtggtggcg
     1261 aaggcggcag cgggcgaagc atcgcgaagc gcgtccagca gcacctcgat ccgcgtggga
     1321 tcaaaggcgt cgtctccatc gagaaaggcg acgtgcgttc cgcgcgcggc agccaatccg
     1381 gcgttgcgcg ccgcgctcgg gccggcgttc tcctggcgga tcaggcgcac ctccctcccg
     1441 aagcgccgga ccacctccgg cgtgtcatcc gtggagccat catcgaccac gatcacctcg
     1501 atccggggat agctttgcgc gagcgccgag caaatcgcgt caccaatgaa ctctgccacg
     1561 ttgtgggctg gaatgatgat gcttaccatc tccccgccct cgcccgccgg aagaggtaga
     1621 accacggtga cagcttggcc aggaagaggg gcagcgaagc ttccgcagtg ttcacccggt
     1681 cgaggcacca ccggtgatag tcgagcccct cgccggcgca cgcggcgcgg tagccggcgg
     1741 cgcgtgccgc ccggcggcag cgatcatcca cctggctgta gggataggcc aggatgtgca
     1801 cgcgctggcc cagctggcgc tgtagatcta ggcgcgaccc gagcagttcc tccgcgaggg
     1861 caccctcgtc caggagcggc agctcgcggt gagtgcgggt gtgcgagccg atccagtgcc
     1921 cgcgccgggc gagatcacgc gcctcttccc acgacatcag gggaaacccg tcgtccgccg
     1981 ccacgtcggc gtcccaggtg gccttccggc cgatgtaatc actcaccagg aagagggtgg
     2041 cccgaaacct gtaggcttcc aggatgggcg ccgcgatgtg gtacgtatcg gcatagccgt
     2101 catcgaaggt gatggcgacg ggtcgaggcg gcagaggcgc tctcccatcc agccattggg
     2161 cgatccgctc gggcgaaacg gcgtggaagc ggaggcgatg gagacagcgc atctgcgcct
     2221 cgaaacgctg gcgtgagagg gtgaagcggc gcagcaatgg gtcgcgctct ccctcgccga
     2281 tgcggtggta catgaggatc ggggggcgcc agccggcccg gtgccggatg cctggccgcg
     2341 ggctcaccga cgcccacgca gcgccattcg ccggcgcgcc tcccgggcga gcgtgcccag
     2401 gtgatggttg cgccacagga acggctggat ctgggtggcc caccagaacg cgggcacgcc
     2461 cgcgagcgtc ttgcgcgcga ccaggaagcg gtccagaacg gttacctccc gcgcccattg
     2521 cagcttgtac tcctctcccc cgcgcccgaa atcgtcgatc tccgcaaggc cgcgcgcccg
     2581 gacgtcgtcg atcaccatcg cctccaggat gtgaccgggg ctgtaatcgg catagctggc
     2641 ggcaaaggtg gagatgtagc cgaggatgcg ccggccatcc agaaagtaga gcccgatgct
     2701 gaccgggtca ccgttcaagg ccagccggca gaggtagagc tgccgggcgc ggaggaggcg
     2761 ctggcagaac gcctggagcc atgcgcggta ctcctcgaag cagaacatgc ttgtctggcc
     2821 gcgcgcctgc cagcgggcca catggatctt cgccatttgc gccagcatct cgtccacgct
     2881 ctcggggtgg gcggccacct caaaggagag gactcccttc tcgcgcagcc ggcgggtgcg
     2941 ccggaggatg ttgtcgcggc gctgcttcga aagcgacttg cgccagtcct cggtgagatc
     3001 catgtatggc gcgatgtcgt tgcgctggcg aaccagcagg attcccgtgt cgcgtgcggg
     3061 cagcaggcgc gtgacgttgc cctgctccgg cacatagcgc aggtcgagca cgtgccagtc
     3121 atcgcactcg cggagcgcgg tccagagcgc gccgagatcc tggggatact ccggatcgct
     3181 cagcgcgtcg caatagtcag aatcgttgtc ggcggcgaag cggagcacgc gcagtgaa
//
LOCUS       DAOSCV010000495         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19071_length_3233_cov_3.209566, whole genome shotgun sequence.
ACCESSION   DAOSCV010000495 DAOSCV010000000
VERSION     DAOSCV010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19071_length_3233_cov_3.209566"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLY56_12125"
     CDS             <1..649
                     /locus_tag="PLY56_12125"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ22274.1"
                     /translation="LATRPEKSIGSDEVWEHATRSLIEAMDARGLNYEVDPGGGVFYG
                     PKIDIKLYDALGREWQGPTVQADFNMPERFDVNYVGPDGAEHRVVMIHRVVLGSMERF
                     VGGLIEHYAGAFPTWLAPVQAILLPIAERHGEYAHQVAAELRAAGLRVQVDDRSEKTG
                     FKIREAQLQKIPYMLVTGDREVEQRSVSVRSRDAGDLGAMSVEGFLRKIQEEVAG"
     gene            736..882
                     /locus_tag="PLY56_12130"
     CDS             736..882
                     /locus_tag="PLY56_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22275.1"
                     /translation="MDGGNICFFDGHVKWYRFEKGPIPAPWAAPYSDDYSIHPPKETF
                     WNVW"
     gene            1083..2795
                     /locus_tag="PLY56_12135"
     CDS             1083..2795
                     /locus_tag="PLY56_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ22276.1"
                     /translation="MSDVRYISREESLRWFREAKLGMFIHWGVYALLEKGEWIQEVEG
                     IQGEEYEKLPPQFTAEKFDPRTWVDLAREAGCRYITFTTKHHDGFCLFNTATTDYNVM
                     NTPCGRDIVRELTEECHRQGLRMHYYYSIMDWHHPDYLPRRSWDKRPADGYDMARYRE
                     YMKEQLRELLTQYGPVGCLWYDGGWMHTPEYMDSVRMNAMVREIQPHILINDRAMTPE
                     DFGTPEQRVPATGLTNPDGSPRVWEACLTITSHWWGYDAHETKFKSPAEVLRIFIDVV
                     SKGGNLLLNVGPKPDGTIQDEFVAVFRALGEWMRVNGEAIYATNASPFRRLPFFGRVT
                     RKGNRLYLHVFDWPRDGVLRLVGLKTEATAAWLLAQPDVRLTLDRDGDTLCIRVPASA
                     PDPVASVVAVELAGEPVVEQLVLKPDADGRVNLPALYADVHGPHGQRARLEAVDGVVQ
                     VGNWINPRDLAAWDFELPEAGEYEVTLNYACPPEQAGATVAAVSYPDERFLGRSGPEE
                     IPDDPAPTVTGTVAATASATDFQRLRLGKLSLPAGKSYLVVKALEMPNGAVMNLRGVE
                     LTQA"
     gene            2792..3058
                     /locus_tag="PLY56_12140"
     CDS             2792..3058
                     /locus_tag="PLY56_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22277.1"
                     /translation="MRRSGGKETSQAGEQGMGERLNPEEILRGLHTGWMGHRLETREE
                     TESTNDDCIALARAGAPAGTVVVTNWQSKLVAIPSGYLVTDILS"
BASE COUNT          563 a         1014 c         1103 g          553 t
ORIGIN      
        1 cctggcgacc cggccggaga aatcgattgg ctccgacgag gtctgggagc acgccacgcg
       61 ctcgctgatt gaggcgatgg atgcgcgcgg gctcaactac gaggtggatc cgggcggagg
      121 cgtcttctac ggtccgaaga tcgacatcaa gctgtatgac gcccttgggc gcgagtggca
      181 gggcccgacg gttcaggccg acttcaacat gccggagcgc ttcgatgtca attatgttgg
      241 gccagacggc gccgagcatc gcgtggtgat gatccatcgc gtggtgcttg gctcgatgga
      301 gcgcttcgtc ggcgggctga tcgagcacta cgcgggcgcc ttcccgacgt ggctggcgcc
      361 ggtgcaggcg atcctcctgc caatcgcgga gcggcatggc gagtacgcgc accaggtggc
      421 cgccgagttg cgcgcggccg gcctgcgcgt gcaggtcgat gaccgctcgg agaagacggg
      481 cttcaagatc cgcgaagcgc agcttcagaa gatcccctac atgctggtga cgggcgatcg
      541 cgaggtggag cagcgttccg tctccgttcg ctcgcgcgat gccggcgatc tcggcgcgat
      601 gagcgtcgaa ggcttcctcc gcaagatcca ggaggaggtg gccggctagc cgccacccca
      661 ggcgggatgg cgcttttctg acgcgcgggc ctgcaggcgt gcctgttggc ccgcgcgtag
      721 tccctcttct tgagaatgga tggcggcaac atctgcttct tcgacggtca cgtgaagtgg
      781 taccgcttcg agaaagggcc gatccctgcg ccctgggctg ccccgtactc cgatgattat
      841 tccatccacc cgcccaagga gaccttctgg aacgtgtggt agccaggctc aggactaacg
      901 tttcgcgggg ctgccggcgc gtgtcactgg cacggccagg cagccccttt cactgtctga
      961 gcccgtcatc ccccggcgca tcgcgcctgg cggaaaagcg cgccacgggc agcaggaagc
     1021 gggcagccgg cccagaaacc accggatcgg gaagccgtga agcccgtgaa gggagagcga
     1081 ccatgtctga tgttcggtat atcagccgtg aggagagcct gcgctggttc cgtgaagcaa
     1141 agctggggat gttcatccac tggggcgtct atgcgctctt ggaaaaggga gagtggatcc
     1201 aggaggtcga ggggatccaa ggcgaggagt atgagaagct gccgccgcag ttcaccgccg
     1261 agaagttcga tccccgcacc tgggtggatc tggcccgcga ggccggttgc cgctacatca
     1321 ccttcaccac gaagcatcat gatggcttct gcctcttcaa caccgcgacg accgactaca
     1381 acgtgatgaa cacgccctgc gggcgtgata tcgtgcgcga gctcaccgag gagtgccacc
     1441 gccagggcct gcggatgcat tactactact ccatcatgga ttggcaccat cccgattacc
     1501 tcccgcgccg gtcgtgggac aagcgccctg cggatggcta cgatatggcc cgctaccgcg
     1561 agtacatgaa ggagcagctt cgcgagctgc tcactcagta tggccctgta ggctgcctct
     1621 ggtacgatgg cggctggatg cataccccgg agtacatgga ctccgttcgc atgaacgcga
     1681 tggtgcgcga gatccaaccg catattctga tcaacgaccg tgccatgacc ccggaggact
     1741 ttggcacccc cgagcagcgc gttcccgcga ccgggctgac caaccccgat ggcagcccgc
     1801 gcgtctggga ggcctgcctc acgatcacct cgcactggtg ggggtatgat gcccacgaga
     1861 cgaagttcaa gagcccggcg gaagtcctcc gcatcttcat cgatgtcgtg agcaagggcg
     1921 gcaacctgct cctgaacgtc ggtccgaagc cggatggaac catccaggat gagttcgtgg
     1981 ctgtcttccg cgctctgggc gagtggatgc gcgtcaacgg cgaggcgatc tacgcgacga
     2041 acgccagtcc attccgccgg ctgcccttct tcgggcgcgt cacgcgcaag gggaaccggc
     2101 tctaccttca cgtcttcgat tggccgcgcg atggcgtgct ccgcctggtc ggactgaaga
     2161 cggaggcgac cgctgcctgg ctgctggcgc agccggacgt gcgcttgacg ctcgaccggg
     2221 atggggatac cctgtgcatc cgcgtgcccg cgagcgcgcc ggacccggtg gcgagcgtgg
     2281 tggccgtcga actcgcgggc gagcccgtgg tggagcagct cgttttgaag ccggatgccg
     2341 atggtcgcgt gaacctgccc gctctctatg ctgatgtgca tggcccccat ggccagcgcg
     2401 cgcgcctgga agccgtggat ggcgtcgttc aggtgggtaa ctggatcaac ccgagagacc
     2461 tggccgcgtg ggacttcgag ctgcctgagg ctggcgagta tgaggtgacc ctgaactacg
     2521 cgtgcccgcc cgagcaggcc ggggccacgg tggccgcggt cagctacccg gacgagcgct
     2581 tcctgggccg cagcgggccg gaggagattc ccgatgaccc cgcgccgacg gtgaccggaa
     2641 cggtcgctgc caccgcgtcc gcaaccgact tccagcggct gcgccttggc aagctctcgc
     2701 tgcccgcggg caagagttac ctggtggtga aggcgctgga gatgccgaac ggcgcggtga
     2761 tgaacctgcg cggtgtggag ctgacgcagg cttgaggcgt tcaggtggca aggagacctc
     2821 tcaggcagga gagcagggga tgggggagcg cctgaacccg gaagagatcc tccgcggcct
     2881 gcataccgga tggatgggcc accggctgga aacgcgcgag gagacggaat cgacgaacga
     2941 cgactgcatc gctctggcac gtgccggggc cccggctggc acggtcgtcg tcaccaactg
     3001 gcagagcaag ctggtcgcca tcccctccgg ctatctggtg accgacatcc tctcctgatt
     3061 ttgacccgga ggcggccgcg cggccgcctc ccttcttctt ttccaacgcc gaaggataaa
     3121 cgatgttcga tttcacggtg agtttcaagg gcgggaaggt cacgaagatc aaggcgaagc
     3181 gggtgatcct caccccgggc tggctgggcc tgtccgagac cgacaacgcc acc
//
LOCUS       DAOSCV010000496         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19092_length_3231_cov_2.848237, whole genome shotgun sequence.
ACCESSION   DAOSCV010000496 DAOSCV010000000
VERSION     DAOSCV010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19092_length_3231_cov_2.848237"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="PLY56_12145"
     CDS             <1..825
                     /locus_tag="PLY56_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22278.1"
                     /translation="GAGKTTTIKMLATLLRPTSGTATIAGFDIVREPEAVRAVIGYMP
                     DFFGVYDDIKVWEYLDFFAAAYKIPTERRHHVVQDVLELTDLTGKRDAYVEELSRGMK
                     QRLCLAKTLVHDPKVLLLDEPASGLDPRARIEIKELLKELKSMGKTILISSHILPELA
                     DFCNKIGIIEKGKLVISGGVQEIMRQVRGGRVLLVRVLDEPDRAIELLETMEGILSAS
                     VDEVHSAIRIEFAGDETAQHALLRRLIENGVRVQSFDEVQTDLEEIFMKVTRGIVS"
     gene            855..2465
                     /locus_tag="PLY56_12150"
     CDS             855..2465
                     /locus_tag="PLY56_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22279.1"
                     /translation="MLQVPAIRPSNPVLWKELRTRIRGNRAYWLLFWYVMLLSIVLFC
                     AYFLKQRMALKQPIEIYADPVGRDLFEVLSVTQAALLTIVTPTLTAGMVTIEREQRTL
                     DLLMLTLLRPREIAEGKLLSAFGFAALLLTASLPLAGICFMLGGISPGEIAVTYLMLA
                     GATLFCGALGLLVSAMARTTVISTAATYMAVLALGLCMGVTIVSSTMDARLFAGFNPM
                     FAVFFGPEQTTFYRWHVPNWIPSLVLTLLAALLATAGAAHKLEAGVSECPGALRWLTW
                     TFFTAMAFCCIGSILGGVPQSTSVEELREAAAILGGTLFLALALLALFFCTGDATEIY
                     GEVPAREAGVSRGPLTRFARSWLFTGGYASGPAYILFLFLTGAIVIVTGFPLAGHTDW
                     VQITGIILAALGVALSGTLLWIAMARFFSVVRGNRLQAGIFTYVTLGVALILLPLLNT
                     DAWKGPAHQPGILAWTLYLNPGCAFFALGSTPEAWKKVDLSLPAGQNSTWVFTFFLYM
                     GLAAIIEVWGQARRRQERARRKVPARAE"
     gene            complement(2480..>3231)
                     /locus_tag="PLY56_12155"
     CDS             complement(2480..>3231)
                     /locus_tag="PLY56_12155"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ22280.1"
                     /translation="ASARRATRMKQQLLAHRPVVVAHRGASVEAPENTLAAFRRALEL
                     GAPAMELDVHRTADGELVAIHDATVDRTTNGTGAVAEMTLEALRQLDAGAWKGKAFAG
                     ERIPTLAEVCRLARGRASLVVEIKAAGIADQVVATLEAEGVLDESFVISFSQETARRV
                     KELRPDTTVGLLSGKAEDLPLVAELGLDAFCPHHAAATEEMARELHAANRLLSVWTVN
                     DEEGMRRMAALGADLITSDNPALALKVLRRE"
BASE COUNT          510 a         1099 c         1016 g          606 t
ORIGIN      
        1 ggcgccggga agacgacgac gatcaagatg ctcgccaccc tcctgcgccc cacgtccgga
       61 acggcgacca tcgccggctt cgatatcgtt cgcgagccgg aggcggtccg cgccgtcatc
      121 ggctacatgc cagacttctt cggcgtctac gacgacatca aagtctggga gtacctcgac
      181 ttcttcgccg ccgcctacaa gatccccaca gaacgccgtc accacgtcgt ccaggatgtg
      241 ctggagctga cggacctcac cgggaagcgc gacgcctacg tcgaggagct ttcacgcggg
      301 atgaagcagc gcctctgcct ggcgaaaacg cttgtccacg accccaaggt gctcctcctc
      361 gacgagccag cctctggtct tgatccccgc gcccggatcg agatcaagga gctgctcaaa
      421 gagttgaaga gcatggggaa gacgatcctc atctcctcgc acattctccc ggagcttgcc
      481 gacttctgca acaagatcgg gatcatcgag aaggggaagc tggtgatcag cggcggcgtt
      541 caggagatca tgcgccaggt gaggggcggg cgggtgctcc tggtgcgggt tctggacgag
      601 ccagatcggg cgatcgagct cctagagacg atggagggca tcctctccgc cagcgtggat
      661 gaggtccact ccgccatccg aatcgaattc gcgggcgatg agaccgcgca acacgccctc
      721 ttgcgccggc tgattgagaa tggcgtccgc gtccagtcgt tcgatgaggt gcagacagat
      781 ctcgaggaga tcttcatgaa ggtgacccgg ggcatcgttt cgtgagatgg cggggccgcc
      841 atagggggag agagatgcta caggtgccgg ccattcgtcc gagcaatccc gtgttgtgga
      901 aggagttgcg cacgcggatc cgcgggaatc gcgcctactg gctccttttc tggtatgtga
      961 tgctcctctc catcgtgcta ttctgtgcct acttcctcaa gcaacgcatg gcgctgaagc
     1021 aaccgataga gatctacgcg gatccggtcg ggcgcgatct ctttgaagtg ctttccgtaa
     1081 cccaggccgc actgctcacg atcgtcacac cgacgctgac cgccgggatg gtcacgatcg
     1141 aacgcgagca acgcactctc gatctgctga tgctcacctt gctccgccca cgagagatcg
     1201 ctgagggcaa gctactctcg gcgtttgggt tcgcggcgct cctgctcacg gcgtcgcttc
     1261 cgctcgcggg gatctgcttc atgttgggcg gcatctcccc gggtgagatc gcggtcacct
     1321 acctgatgct cgcgggcgcc accctcttct gcggggcgct cggcctgctc gtctccgcga
     1381 tggcacggac cacggtgatc tcaaccgctg cgacctacat ggcggtcctt gccctcggcc
     1441 tctgcatggg cgtcaccata gtgtctagca caatggacgc gaggctcttc gccgggttca
     1501 acccgatgtt cgccgtcttc ttcggcccag agcagacgac tttctaccgc tggcacgtgc
     1561 ccaactggat tcccagcctg gtgctcacct tgctggccgc gcttctggcc accgccggcg
     1621 ccgcgcataa gctggaagcg ggcgtatcgg aatgcccggg cgccctccgc tggctcacct
     1681 ggaccttctt cacggcgatg gcgttctgtt gcattgggag catcctcggg ggagtgcccc
     1741 agagcacctc ggtggaagag ttgcgcgaag ccgcagcaat tctaggcggc acgcttttcc
     1801 tcgcgctcgc gctgcttgcg ctcttctttt gcaccggcga tgccaccgag atctacggcg
     1861 aggtgcccgc gcgtgaagcc ggcgtttccc gcgggccgct gacgcgtttc gcgcgctcgt
     1921 ggctctttac ggggggctat gcctcgggcc cggcctacat cctctttctc ttcctcaccg
     1981 gggccatcgt gattgtcacc ggcttcccgc tcgccggaca cacggactgg gttcagatca
     2041 ccggaatcat tctggctgcg ctcggcgtcg cgctctctgg cacgctcctc tggatagcga
     2101 tggcgcgctt cttctccgtc gtgagaggaa accggctcca ggccggcatc ttcacctatg
     2161 tgacgctcgg cgtcgcgctg atactcctcc ccctcctcaa caccgacgca tggaaagggc
     2221 ccgcgcatca accaggcatc ctcgcctgga cgctctatct caacccgggc tgcgccttct
     2281 tcgccctcgg gagcacgcca gaggcctgga aaaaggtcga cctgtcgctc ccggctgggc
     2341 agaactccac ctgggtgttc acgttcttcc tctacatggg gctcgccgcc atcatagagg
     2401 tgtgggggca ggcgcggcgg aggcaggagc gcgcgcgccg gaaggtaccg gcgcgcgctg
     2461 agtgaccgga gccgcgtggc tactcgcggc ggagcacctt cagggccagg gcggggttgt
     2521 cgctggtgat aagatcggcg ccgagcgccg ccatccggcg catcccctcc tcgtcgttca
     2581 ccgtccagac gctcaggagc cgattcgccg cgtggagctc gcgcgccatc tcctccgtgg
     2641 ccgcagcatg gtgggggcag aatgcgtcca ggcccaactc ggccaccagg ggcaggtcct
     2701 ccgccttgcc ggaaaggagg ccaacggtcg tgtccgggcg gagctccttc acgcgccggg
     2761 cagtctcctg ggagaaggag atgacgaagc tctcgtccag caccccctcc gcctcgagcg
     2821 tggcgaccac ctgatcggcg atcccggcgg ccttgatctc aactacgagc gacgcccgcc
     2881 cgcgcgccaa gcggcacacc tccgccaggg tgggaatccg ctcgcccgcg aacgccttgc
     2941 ccttccacgc gccggcatcc agctggcgca gcgcctccag cgtcatctcc gcgacggcgc
     3001 cggtgccatt cgtggtgcgg tccacggtgg catcgtggat cgcgacgagc tctccatccg
     3061 cggttcggtg gacatcgagc tccattgcgg gcgcgcccag ctccagggcg cgtcggaacg
     3121 cggcgagcgt gttttcaggc gcctcgacag acgccccccg gtgcgccacg accaccgggc
     3181 ggtgtgcaag cagttgctgc ttcattcggg ttgccctcct ggcagatgct t
//
LOCUS       DAOSCV010000497         3228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19109_length_3228_cov_3.978254, whole genome shotgun sequence.
ACCESSION   DAOSCV010000497 DAOSCV010000000
VERSION     DAOSCV010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19109_length_3228_cov_3.978254"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLY56_12160"
     CDS             complement(<1..701)
                     /locus_tag="PLY56_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22281.1"
                     /translation="MALTPVRIWLGPAGSGKTHRARSLAGERCWAIVPSEGATRAFPK
                     LASGESAAFTFRRLAERMTGLGPREIASPALRSFLLHQAVRHAVTPGSYFERAASQPG
                     FAPAIGDLIREFKQGEVTVDAFLDAASACAAEVDDPCFLEKCECIAAVWCEYDRLLSR
                     HETRDADDLLAQAMAAVDEWRDSPERVVVDGFTSFTSQQIGLLAGLQRRGIHLEICLT
                     YDPSRPVLFARVSRTR"
     gene            complement(975..1778)
                     /locus_tag="PLY56_12165"
     CDS             complement(975..1778)
                     /locus_tag="PLY56_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOJ22282.1"
                     /translation="MPAARAVILGVVQGLTEFLPVSSSAHLVLLARLGRWRKDSLDLE
                     VALHLGCLLALLAAFRGEWSWLLRSGLTGTDPNARALLASLLLACIPASVAGVVLERY
                     ARGTFRRLPQIAALTALMGGVLILADHVPGSDRGLLQVTWRDGLVIGLAQALALLPGV
                     SRSGVTITAGVLLGLSLPAAVSFSFLLAAPILAGAGLWGLRHLSWRGIPRGERQTMAI
                     GLITAALVGYFAITFMLRVIAHIHLTPFGLYRLLFALLLWRLASTRQAD"
     gene            1985..2776
                     /locus_tag="PLY56_12170"
     CDS             1985..2776
                     /locus_tag="PLY56_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ22283.1"
                     /translation="MIIEAREDVVTLRGNLLENMWPAIQAATNLLLKQHPAGIIIDCG
                     HLETASGAGVQTFVDAMHYIQKQGKRVLLANVPEEIMQVLREVPGAGSQLPVAKTIED
                     ARRSLELQGIQPAEPEEKPGKVILVPLMEMLDWEHGIDLACRIAAGARAEIHMVYLLE
                     VPLSQPVGAALPEAEAAAKEALNRARARAGQCGMPAVAHVERTRELGAGILQAAKDLH
                     ADVIVAAIRPTAEGGDPRLERVLTVLLQKAEGEVVIDRAPTEPQA"
     gene            2994..3068
                     /locus_tag="PLY56_12175"
     tRNA            2994..3068
                     /locus_tag="PLY56_12175"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3027..3029,aa:Arg,seq:ccg)
BASE COUNT          615 a         1076 c         1106 g          431 t
ORIGIN      
        1 cgggtgcgcg aaacgcgagc gaagaggaca ggccgcgagg ggtcgtaggt caggcagatc
       61 tccaggtgaa tgccccggcg ctgcaggccc gcgaggaggc cgatctgttg ggaagtgaac
      121 gacgtgaagc catccaccac cacgcgctcc gggctatccc tccactcatc gacggcggcc
      181 atcgcctggg ccagcaggtc atcggcgtcg cgcgtttcat gccgggaaag gagccggtcg
      241 tactcgcacc agaccgccgc gatgcactcg cacttctcca gaaagcacgg atcgtccacc
      301 tccgcggcgc atgcggacgc cgcgtcgagg aaggcgtcga cggtgacctc cccctgcttg
      361 aactcgcgga tcaggtcgcc gatggcggga gcgaagcccg gctggcttgc ggcgcgctcg
      421 aagtaagagc ccggagtaac ggcgtggcgc accgcctgat gcagcaagaa ggaacgcaac
      481 gccggcgaag cgatctccct gggcccgagg cccgtcatcc gctcggccag ccggcggaag
      541 gtgaaggcgg cgctctcgcc ggacgcgagc ttcgggaagg cgcgcgtcgc gccttccgag
      601 ggcacaatcg cccagcacct ctccccagca agcgagcgag cgcggtgcgt cttccccgag
      661 ccagccggac ccagccagat ccggacgggc gtcaaagcca cgatggagtg cctcccccca
      721 ggtggggccg ttctcctcct ggccccacga cacacatctg ttcaccacgc gcccgcgtca
      781 ttcctcccgg aaagggaggc aacgcgcctg gatctcaccg ggaggcgatg ctcgcgaaga
      841 tgaccgccgg aggcgcctcg tccagatagg cgaggctgaa gatgaaggtg ccgttgtcgg
      901 acgaggcgac caggtcgagg cgctagtcgc tcacctgcct caggacacct ccacacggtg
      961 tcccttccag gcggctaatc ggcctgccgg gtcgaggcga ggcgccagag gagaagcgcg
     1021 aaaaggaggc gatagagacc gaagggcgtc aggtggatgt gggcaatcac gcgcaacatg
     1081 aaggtaatcg cgaagtaacc taccagggcg gcggtgatga ggccaatcgc catcgtctgg
     1141 cgctccccgc gtgggatccc gcgccaggaa aggtggcgca ggccccatag gccggcgccc
     1201 gcaagaatcg gcgccgcgag aaggaaggag aagctcaccg ccgccggaag cgagaggccc
     1261 agtaggacgc cggcggtgat cgtcaccccg gatcgcgaga cgccaggcaa gagagcgagc
     1321 gcctgcgcca gcccgatgac caggccatcg cgccatgtga cctgcaagag tccacgatcc
     1381 gagccgggca cgtggtcggc gaggatgagc acaccgccca tcagcgcggt gagcgcggcg
     1441 atctgcggca gccgccggaa cgtgccgcga gcatagcgct ctagcaccac ccccgccacc
     1501 gaggccggga tgcacgcgag gagaagcgag gcgagcagcg cgcgcgcgtt gggatcggtg
     1561 ccggtgaggc cgcttcgcag gagccagctc cactcgccgc ggaaggcggc gagaagcgcc
     1621 aggaggcacc ccaggtgcag tgccacttcc aggtcgagcg aatccttgcg ccagcgcccc
     1681 aggcgcgcca gaagcaccag gtgggcagaa ctgctcaccg gcaggaactc cgtgagcccc
     1741 tgcaccactc ccaagatcac cgctctggcc gctggcaccg ctttcccttc tagcacggcc
     1801 cgactttccg gttaagccgg ggcggaagca cgcgcccctc atcataccac gccggccctc
     1861 aaaacgcaaa acgcacccgg tgcgcggatg cgggcagggg cggcgggtac tcctggagaa
     1921 tgagaggggg ttgagggggc tcgccgcgtt cacggagccc tcgcctggtt gatggagagc
     1981 ccagatgatt attgaagcgc gagaggatgt ggtgaccctg cggggcaacc tgctggagaa
     2041 tatgtggccc gccattcagg cggcaacgaa cctcctgctc aagcagcacc ccgccgggat
     2101 catcatcgac tgcggccacc tggagaccgc gtccggcgca ggcgtgcaga ccttcgtcga
     2161 cgcgatgcac tatatccaga agcaagggaa gcgcgtgctc ctcgccaatg ttccggaaga
     2221 gatcatgcag gtgctgcgcg aagtgcccgg cgctggctcg cagcttcccg tcgccaagac
     2281 gattgaggat gcgcggcgct cgttggagct gcaggggatc cagccggccg agccggaaga
     2341 gaagcccggg aaggtgatcc tggtgccgct gatggagatg ctcgactggg agcacgggat
     2401 cgacctcgcc tgccgcatcg cggcaggcgc gcgcgccgag atacacatgg tctatctgct
     2461 ggaggtgccc ctgagccagc cggtgggcgc ggcactcccc gaggcggaag ccgccgctaa
     2521 agaggctcta aaccgcgcgc gcgcccgcgc gggccaatgc ggtatgccag cggtggcgca
     2581 cgttgaacgc acgcgcgagc ttggcgccgg aatcctccag gccgcgaaag acctccatgc
     2641 cgatgtcatc gtcgccgcaa tccgtcccac cgccgagggc ggcgacccac gcctggaacg
     2701 cgtgctcacc gtgctgctcc agaaggcgga gggcgaggtg gtcatcgacc gggccccgac
     2761 ggagccacag gcatagcgcc ggcttcccct tctccgaccc cgcgccagcc ggcgatacta
     2821 tgtatggatg aaggggccgg acacggatgg gaaggatgcg ggcgccctgg atcgtgcgcg
     2881 ctcctggatc gagacggtca ccggcgtgac gattgtggcg aacgaaacgc gatcaacctc
     2941 ttgtcacggc gcgcggggta tgctataatg taaccgtcaa aacatatggg aatgccctcg
     3001 tagctcagtg gatagagcgc gggtctccgg aacccgaagc gtaggttcga ttcctatcga
     3061 gggcaccatt caagagggcg ccgagagcgc cctcttcctc tgtacgggcc acaggcgagc
     3121 taccggcgca gcccgctgcc cggcttccct gggattccga cgtaaggaga aggccgcctg
     3181 cacgcgcgga gctcgaagct ccgctctgct ttccagcaaa gccccctc
//
LOCUS       DAOSCV010000498         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19124_length_3227_cov_2.873581, whole genome shotgun sequence.
ACCESSION   DAOSCV010000498 DAOSCV010000000
VERSION     DAOSCV010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19124_length_3227_cov_2.873581"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PLY56_12180"
     CDS             complement(<1..919)
                     /locus_tag="PLY56_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOJ22284.1"
                     /translation="MSVKFLISGLSDEEMMALQGKVSGAELLLAQVARLSPEEIDLIR
                     QAAPDAELLVAASHAEAMALAPEADALFGYLNADLFRAAPRLRWVQAPSAGVEVYLFP
                     ELRHSHVILTNARGLAAAQMADHTLALLLALTRKIPAMLRDMGRRDWGSDDQIAPELA
                     GQTLLIVGLGSVGRLVAQRASAFDMRILATGFEGSPSIMRVDALHPPDNLTHILPHAD
                     HVAICCPLTPATYHLFDAPMLRRMKQSAFLINVARGGIVDHEALATALEAGELAGAGL
                     DVTEPEPLPDGHRLLTLENVAELLMVREAL"
     gene            1088..2410
                     /locus_tag="PLY56_12185"
     CDS             1088..2410
                     /locus_tag="PLY56_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOJ22285.1"
                     /translation="MRKHFFFLIAALAGGLMVPAAASQVADSDKDGLPDEVEERLGTD
                     PRRPEALEQIATFPATVKERPELDIVRVDFGNVGRDRWLWAVHTAKPYTFENAGLILY
                     LDLDADPKTGRRDMGCEVTVGHSLGRPTANGFEPDGSPRAVPAPRVALVGGVLYLCHD
                     ATIRQEGGRTRLRFSILSETREPHRTVDGTGWVTALGPANSNRKPPVTLDELVANEGF
                     ERTEGLDLIWQLQADPANLVFSSVEAEREGMDYYDAEYRWPAVRGAGGSLTVTVPRAG
                     RFHPAVVVYDAAGRETYELRIDGKVAGRFIAAEDDRRQRIYFLAKPVEFKGGEKLTLR
                     TGGGGAHITEDLFLLAKKPPVRGRAFEIRHVQAEYVNRAGEGAIRLTWITTWPAQCTV
                     TCGGQKLTEEKPVANHRVYIPAPPREQPGGIESRRLGLMASRCRTRAR"
     gene            2389..>3227
                     /locus_tag="PLY56_12190"
     CDS             2389..>3227
                     /locus_tag="PLY56_12190"
                     /inference="COORDINATES: protein motif:HMM:NF039329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22286.1"
                     /translation="MSHAGTVTLAPPKPFAGTAKHERIPLKVENPYPFPQDGFLVTSG
                     VPFAKGELGDPDHVRLLDGAGREVPLQPVVAGRWGDGSIKWLRLSFSATVDAGKTATH
                     TLEYGTQVSRAPARTPLACVWKGDTLQVETGPLRVEFERTRSGFPIAVWYDHNADGTF
                     TSDESLTGDLPISARLHDTKAVSYTTLHAPRRIEIEESGPVRAVVKVTGSYQSGEGKP
                     WFAYTTRFVFHAGSAMVRVHHTWGADDPGEEFVEFERIGLEFPLAAREEWSWRIGLGH
                     GQE"
BASE COUNT          532 a         1062 c         1125 g          508 t
ORIGIN      
        1 cgagggcctc gcggaccatc aacaattccg ccacgttctc cagggtcagc aggcgatggc
       61 cgtctggcag cggctccggt tcggtcacat cgagccctgc gcctgccagt tcgcccgcct
      121 ccagcgccgt ggccagcgcc tcgtggtcta cgatcccccc gcgcgcgacg ttgatcagga
      181 aagcgctctg cttcatccgg cggagcatcg gtgcgtcgaa gaggtggtag gtagcggggg
      241 tcagcgggca gcagatcgcc acgtggtctg cgtggggcag gatgtgcgtg aggttgtcgg
      301 gtggatggag agcatcgacg cgcattatcg acgggctgcc ctcgaacccg gtggccagga
      361 tgcgcatatc gaaggcggac gcgcgctggg cgaccagccg tccgacactg ccgagcccga
      421 cgatgagcag ggtctgcccg gccaactctg gcgcgatctg gtcgtcgctt ccccaatccc
      481 gtcttcccat gtcgcgaagc atcgccggta tcttccgggt gagcgccaaa agaagcgcca
      541 gggtatggtc ggccatctgc gctgccgcca ggcctcgggc gttcgtcagg atgacgtggc
      601 tgtgccgtag ctcggggaag agatagacct ccacgccggc gctgggcgct tgcacccagc
      661 gcagccgcgg ggccgcgcgg aagaggtcgg cattgaggta gccaaagagg gcatctgctt
      721 ccggtgccag ggccatcgct tcggcgtgcg atgccgccac caggagctcc gcgtccggcg
      781 ccgcctggcg gatgaggtcg atctcctcgg gcgagaggcg cgccacttgc gctaacagca
      841 actcggcgcc ggataccttt ccctgcagcg ccatcatctc ctcgtccgaa agccccgaga
      901 tgagaaactt caccgacact gtgacgcccc ctcccttcgc gcgtcgcgtg agctaatccg
      961 gccatccaca ccgtgcgctc cgcgggagaa gcatgcaggc tatatgccag agcgcgagcc
     1021 gcgcgataca ggaggaacca ggctggcctc ctcgaatgga tgggcaagca gagcgggagg
     1081 agaagccgtg aggaagcact ttttcttctt gatcgccgcc cttgcaggcg gattgatggt
     1141 gccggccgcc gcgtcccaag tggcagatag tgataaagac ggcctgccgg atgaggtgga
     1201 ggaacggctt ggcacggatc cccgccggcc ggaggcgctg gagcagatcg ccactttccc
     1261 tgccacggtg aaagagcgcc ccgagctcga tatcgtccgc gtcgatttcg gtaacgtggg
     1321 gcgcgaccgg tggctgtggg cggtccacac ggcgaagcct tacacctttg agaacgccgg
     1381 cctgattctt tacctggatc tggacgccga cccgaagacg gggcgccgcg acatgggctg
     1441 cgaggtgacg gtcgggcact cgctgggccg gccaacggcg aacggtttcg agccggatgg
     1501 ctcgccgcgc gctgtgcccg cgccgcgcgt tgccctggtg ggtggagtcc tctacctctg
     1561 ccatgacgcc accatccggc aggagggagg gcgcacgcgc ctccgcttct ccatcctctc
     1621 cgaaacccga gagccgcacc ggaccgtgga tggcacggga tgggtgacgg cgctcggccc
     1681 ggccaactcc aaccggaagc cgccggtgac gctggatgag ctcgtcgcga acgagggctt
     1741 cgagcgcacc gaggggctcg acctcatctg gcagctccag gctgatcccg cgaacctggt
     1801 cttctccagc gtcgaagccg agcgcgaggg gatggactac tacgacgccg agtaccgctg
     1861 gccggcggtg cgaggcgcgg gcggcagcct gaccgtgacc gtgccccgcg ccgggcgttt
     1921 ccatcccgcc gtcgtggtct acgatgccgc cgggcgcgag acatacgagc tgcgcattga
     1981 tggcaaggtc gcagggcgct tcatcgccgc cgaggatgac cggcgccagc ggatctactt
     2041 cctcgcgaag ccggtggagt tcaaaggggg cgagaagctg acgctccgca ccggcggggg
     2101 cggggcgcac attaccgagg acctcttcct cctggcgaag aagccgcctg tgcgcgggcg
     2161 cgctttcgag atccgccatg tgcaggcgga gtacgtgaac cgcgcgggag agggcgccat
     2221 ccgcctcacc tggatcacga cctggcccgc tcagtgcacg gtgacctgcg gcggccagaa
     2281 gctgaccgaa gagaagcccg tggccaacca ccgggtctac atccccgcgc cgccacggga
     2341 gcaacctggc ggtatcgaat cgaggcgcct cggcctgatg gcaagcaggt gtcgcacgcg
     2401 ggcacggtga ccctggcgcc gccaaagccg ttcgccggca ccgcgaagca cgagcgcatc
     2461 cccctcaaag tagagaaccc ctatcccttc ccccaggacg gcttcctggt cacctcgggc
     2521 gtgccgtttg ccaaggggga actgggcgat cccgatcacg tccgtctcct tgatggcgcg
     2581 ggccgggagg tcccgctcca gccggtggtg gcgggccgct ggggcgatgg cagcatcaag
     2641 tggttgcgcc tctccttctc ggcgaccgtg gacgcgggga aaaccgcaac ccatacgctg
     2701 gagtacggca cccaggtaag ccgcgcaccg gcgcggacac ccctcgcctg cgtctggaag
     2761 ggcgatacgc ttcaggtgga gacgggcccg ctccgcgtcg agttcgagcg aacccggtcc
     2821 ggctttccca tcgccgtgtg gtatgaccac aacgccgatg gcacctttac ctcggatgag
     2881 agcctgacgg gcgacctgcc gatcagcgcc cgcctgcacg atacgaaagc tgtctcctac
     2941 accaccctcc acgcgccgcg tcggattgag atcgaggaga gcggtcccgt gcgcgcggtg
     3001 gtgaaggtga cggggagcta ccagagcggg gaagggaagc cgtggttcgc ctacacgacc
     3061 cggtttgtct tccatgccgg ctcggcgatg gtgcgtgtgc atcacacgtg gggcgcggat
     3121 gatcccggcg aggagtttgt cgagttcgag cgcattggcc tggagttccc gctcgcggcg
     3181 cgggaggagt ggtcgtggcg gattggcctg ggacatggcc aggagtg
//
LOCUS       DAOSCV010000499         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19174_length_3222_cov_3.299021, whole genome shotgun sequence.
ACCESSION   DAOSCV010000499 DAOSCV010000000
VERSION     DAOSCV010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19174_length_3222_cov_3.299021"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="PLY56_12195"
     CDS             complement(<1..1159)
                     /locus_tag="PLY56_12195"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ22287.1"
                     /translation="MRTARDLLWHLPRRYEDRRRFSPIRLLSHGAAALVSGRVTGAST
                     ERRRGLVITRVLLVDDSGSAHLTWYNQPYMEKRFPVGTHLVAYGKVERRFGAVQILNP
                     ECEVVTGNDLAGMGRIVPIYPLTEGLSQQVVRNVLFGLMELTARVPDSLPASVRERCA
                     LVSLPVALRDIHFPETWEACEAARRRLVFEELFRVQLRLAARKVAAVSHPGIAFRVGE
                     EILAEIGEMLPFELTPAQARVLEEIRADMQEPRPMHRLLQGDVGSGKTAVAAAVALAA
                     VRNGYQAALMAPTEILAEQHFLNLERLFRPRGIVVDLLTGSGGEKDRDAVRARLRTGE
                     TGLVVGTHALIQEDVSFARLGLVIVDEQHRFGVLQRQSLWEKGHSATVPDLL"
     gene            1658..2470
                     /locus_tag="PLY56_12200"
     CDS             1658..2470
                     /locus_tag="PLY56_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020097439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ22288.1"
                     /translation="MSPPPCTQVYAAVALFCFLLLAGAGTFLRRRLPAPPPPLPVISA
                     SAPAKAEVHPSARLPSKEIPVAPVPDRPPVVVHVAGCVRQPGLYSFPEGARVYDAIKR
                     AGGAKEGADLDAINLAACLRDGVQVYVPRKSVQQAQRAPTVRVSRSQRGDGAWIAPES
                     IRLEPLPPAPAGRMPLERHEPAPVPPAREEDTPPRAERPAKIRDPSEGRVNINTADAL
                     ELQRLPHVGPAIAQRILEYRKENGPFRSVDQLLEVRGIGEKYLARLRDLVEL"
     gene            2540..2797
                     /locus_tag="PLY56_12205"
     CDS             2540..2797
                     /locus_tag="PLY56_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22289.1"
                     /translation="MGQQDRRETRHTLLPGKVERGEEIDTIKRGLPAEETAVRQAIGA
                     AESGKSPGVSIAQTAEEIDTDTPQEPPEGDVADGSGGLGGL"
     gene            complement(2844..>3222)
                     /locus_tag="PLY56_12210"
     CDS             complement(2844..>3222)
                     /locus_tag="PLY56_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22290.1"
                     /translation="VVDAEEISHLLAGMGDHFVRAWFDGEIPSTAILPPQDAARQQWH
                     AMRGVAREAPAGSLTLVCTHDWNIMLVRETYLGVRHEEQGWAEFLDGPVLVLHNGECR
                     LTWRDRTTAMPLFAPASDRAHTA"
BASE COUNT          547 a         1101 c         1069 g          505 t
ORIGIN      
        1 ccagaaggtc cggcacggtg gcggagtggc ccttctccca aagcgactgc cgctgtagca
       61 cgccaaaacg atgctgttca tcgacaatca ccagcccaag ccgggcgaag ctgacatcct
      121 cctggatgag cgcgtgcgtg ccaaccacga ggccggtctc gcccgtgcgc aggcgggcgc
      181 ggaccgcatc gcggtccttc tcgccgccac ttcctgtgag aaggtccacc acaatccccc
      241 tggggcggaa gagccgctcc aggttcagaa aatgttgctc ggccaggatc tcggtcggcg
      301 ccatcagcgc cgcctgatac ccgttgcgca cggcagccag cgccaccgcc gcagcgactg
      361 ccgtcttgcc ggagcccaca tcgccttgca ggaggcgatg catcggccga ggttcctgca
      421 tatccgcgcg gatctcttcg agcacgcgcg cctgtgccgg cgtgagctcg aaggggagca
      481 tctcgccaat ctcggcgagg atctcctctc ccacacggaa ggcgatgcct gggtgtgata
      541 ccgccgccac cttccgcgcg gccagccgaa gctgcacccg gaagagctcc tcaaaaacca
      601 ggcgccgccg ggctgcctcg cacgcttccc atgtttcagg gaagtggatg tcgcgcaacg
      661 ccacaggcaa actcaccagc gcgcatcgct ctcgcacgct cgcgggcagc gagtccggca
      721 ccctggccgt cagctccatc aggccgaaga gaacgttccg caccacctgc tggctgagcc
      781 cctcggtgag cgggtagatg ggcacgatcc gccccatccc cgccaggtcg ttcccggtga
      841 ccacttcgca ttctgggttg aggatctgca cggcgccaaa ccgccgctcc accttcccat
      901 aggcgaccag gtgcgtgcca acggggaacc gcttctccat gtagggctgg ttgtaccagg
      961 tgaggtgggc cgacccgcta tcgtcgacca ggaggactct cgtgatcacc aggccgcggc
     1021 gtcgctcggt agaggccccg gtgacgcggc cagacaccag tgccgcggct ccatgcgaga
     1081 ggagacggat tgggctgaac ctccgccggt cctcgtagcg acgaggcaga tgccagagca
     1141 gatcacgcgc tgtgcgcacg ccaaggcgct cgaatgcttc ggcgcgccgt ggccccactc
     1201 ccttaaggta ctgcaccggg gagtcgagcg cggtagcgcc gcccgtggca taacccacgg
     1261 aatggggggg ggtgcccctc gatctcccag cccggatatg tgacgttcgc ccgaggggct
     1321 gccgctccac cggagaagac tcccttcgga acccccttat tatactggcg cctctatgcc
     1381 ttgtcaaggc gagcccgagg tggcctcgcc tgcgggggcc ccagcccgga acgccacacc
     1441 ctcctgccgg cgcctaccac gcacctgcgc ggaggaggtg ccgacgggcc tttgcaggca
     1501 caaaaaagga cggctccgcg ccgtcaatct cagtatagca cggatcgcct gtgctgtaaa
     1561 ggcgctgtcg tctggagagc gcgcggggga ttgacaacaa atacctacgt aggctataat
     1621 aaggcgagct tgggctactg ccccctggag gcgaacggtg tcaccgccac cttgcaccca
     1681 ggtctatgct gccgttgcgc tgttttgctt cctgctcctc gcaggtgccg gcacgttcct
     1741 gcgccgccgt ctgcccgcgc ctccgccgcc cctccccgtc atatccgcct cggctcccgc
     1801 caaagcagaa gtgcatccct cggctcgtct gcccagcaag gagatcccgg tcgctcccgt
     1861 gccggaccgc ccgccggtcg ttgtccacgt tgccggctgc gtgcgccaac ccggtctcta
     1921 ctccttcccg gaaggagccc gtgtttacga cgcgattaag cgcgcggggg gcgccaagga
     1981 gggcgccgat ctggatgcga tcaacctggc ggcgtgcctg cgcgatggcg tccaggtcta
     2041 cgtgccgcgc aagagcgttc agcaggcgca gagagcaccc accgtgcgcg tgtcacgctc
     2101 acagcgtggg gatggggcct ggatagcccc agagtcgatt cgcctggagc cgcttccgcc
     2161 tgctccggcc gggcgcatgc ccctggaacg gcatgaaccc gcgccggtgc cccccgcgcg
     2221 cgaggaggac acgccgccgc gagcggagcg ccctgccaag atccgtgatc cttcagaggg
     2281 ccgggtcaac atcaacaccg ccgatgcgct tgagttgcag cgtctcccac acgtcggacc
     2341 ggcgattgcc cagcgcatcc tggagtaccg gaaggagaac ggccccttcc gctcggtgga
     2401 tcaactcctg gaggtccgag gcattggtga aaagtacctg gcccggctcc gcgatctcgt
     2461 cgagctgtag tgcccatgcg gtttgggccg ctggtgcgcc ggggaagagg ggagagagcc
     2521 gtggtggaag ggagacgcga tgggccagca ggatcgaaga gagacccgcc acacgctact
     2581 tccgggcaag gtggaacggg gcgaggagat cgatacgatc aagcgcggcc tgccggcgga
     2641 ggagacggcg gtccggcagg cgattggcgc cgctgaatcg ggcaagtcgc cgggcgtatc
     2701 gattgctcag acagcggagg agatcgatac cgatacgccc caggagccgc ccgagggcga
     2761 cgtggcggat ggtagcggtg gcctcggggg gctgtgagcc cccctcagag ccggctatgc
     2821 ctgccgcatc cggcctacct atctcaggcc gtgtgtgcgc gatctgatgc gggtgcaaag
     2881 agcggcatcg cggtggtgcg gtcgcgccag gtcaggcggc actcgccgtt gtgcagcacg
     2941 agaacagggc catcgaggaa ctcagcccag ccctgctctt catggcgcac gccgaggtag
     3001 gtctcgcgaa cgagcatgat gttccagtcg tgagtgcaga cgagggtgag gctgccggcg
     3061 ggagcttccc gggccacgcc gcgcatcgcg tgccattgct ggcgggcggc gtcctgcggc
     3121 ggcaggatcg cggtggatgg gatctcgcca tcgaaccagg cgcgcacgaa gtgatctccc
     3181 attccggcca agaggtggga gatctcctcc gcatccacga cg
//
LOCUS       DAOSCV010000500         3221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19181_length_3221_cov_2.885344, whole genome shotgun sequence.
ACCESSION   DAOSCV010000500 DAOSCV010000000
VERSION     DAOSCV010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19181_length_3221_cov_2.885344"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLY56_12215"
     CDS             complement(<1..374)
                     /locus_tag="PLY56_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22291.1"
                     /translation="MAYDPAKHLIRIQGNREYLPVAQRLVWFREAHPDWGIETKIEVL
                     DIDAGLAVFSATVINESGKIMARATKMETARGFPDYIEKAETGAIGRALALCGFGTQF
                     APELEEGERIVDSSRRIRVSCQS"
     gene            505..1539
                     /locus_tag="PLY56_12220"
     CDS             505..1539
                     /locus_tag="PLY56_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HOJ22292.1"
                     /translation="MHSDLPAPAGYFLERLRALQLALRDHVCACRDAASPEAMAAVGE
                     ARDGDTLYAIDLHSEEILLEHCARWAEEMPLALIAEGIPGNGWRIFPEGCAPEAARFC
                     LIVDPIDGTRGLMYDKRSAWVLCGVAPNRGEATTLSDIVIAMQTEIPTTRQYLADRLW
                     AVRREGARAERSNLLTGESRAYTPQPSRAESLAHGFAGITKFFPGARDLVASLEETLF
                     ERILGTPADGNPLVFDDQYISTGGQLYELIVGHDRFNADLRPLALAAKYPAASRHRLC
                     CHPYDFCTELIAREAGVIVTDPWGAEVTCPLDIRVDLAWVGYANRKIQAQVGPVLQEL
                     LRKWEGGVPG"
     gene            complement(1591..2301)
                     /locus_tag="PLY56_12225"
     CDS             complement(1591..2301)
                     /locus_tag="PLY56_12225"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HOJ22293.1"
                     /translation="MMKRKALVMTLLLSLAGAGGWADTSMLGTPGGLVVPHARVLPEG
                     RLQTTVSAVWIDRNDETKDNEWYSVNFGLLGTAEVGLSHVAYSSRKARDIVVHGKWLA
                     LPEKDRLPAVAIGVTDITGEVVSDPSVYLVASKLLWSPRDEFTGEARHPLWGHLGIGS
                     GIYGDSPFAGIEWGVARNTRLLVEWGKNLSYGRGDYLFHVGAQYQWRDRFTLSAGAMD
                     MRYPAVSLTARLDDLFLQ"
     gene            complement(2385..2582)
                     /locus_tag="PLY56_12230"
     CDS             complement(2385..2582)
                     /locus_tag="PLY56_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22294.1"
                     /translation="MSLEHGERFEECAGPGECRGFLLLLAVVTLVVLAGLAWFCAYPP
                     SRGAATGDFTVRAVLPGADER"
     gene            complement(2611..>3221)
                     /locus_tag="PLY56_12235"
     CDS             complement(2611..>3221)
                     /locus_tag="PLY56_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22295.1"
                     /translation="EVPIASVAHNAARPVGLGQSFTVTMAGKPRGTATFSIGTMKTGI
                     PMTETAPGIYQGRYTAEKGDVAYAARIVAQLVTETGEQFTRECSEPITILTVPPRAPS
                     ISAPSEDEVVEGPLTVHGKAQPGVTVRVQVVRKTRTLGLWSDHDVLAAQEVKVDSDGG
                     FVTGKLQVPRPRREGEIVTIQAVAIDPVGHRSEIASVKVKLQ"
BASE COUNT          556 a         1118 c          989 g          558 t
ORIGIN      
        1 gactggcagc tcactcgtat ccgcctcgac gagtcgacga tccgctcgcc ctcctccaac
       61 tccggcgcga actgcgtgcc gaaaccacac agcgccagtg cccgcccaat cgctcccgtc
      121 tccgccttct cgatatagtc tggaaaacca cgcgccgtct ccatcttcgt cgcgcgcgcc
      181 atgatcttcc cgctctcgtt gatgaccgtc gccgagaaga cggccagccc cgcgtcgata
      241 tcgaggacct ctatcttcgt ctcgatgccc caatccgggt gcgcctcgcg gaaccacacc
      301 aggcgctgcg ccaccgggag gtactcgcgg ttcccctgga tacggatcag gtgcttcgcc
      361 ggatcatatg ccatttcttc ctcctcacac tcgaaaagca ccaaccgtga ctattgttct
      421 atgcgaacag gcgttttcct tctttccgct ctgcaggagg cgaagccatg gagaaggaag
      481 aagggaggtg gggaggagtg ggagatgcat tcagatctgc ctgcgcccgc gggttatttc
      541 ctggagcgcc tgcgcgcgct ccaattggcg ctgcgcgacc acgtctgcgc ctgccgagat
      601 gccgcatcgc ccgaggcgat ggctgcggtg ggtgaagcgc gcgacggcga tacgctctac
      661 gccattgacc tgcactccga ggagatcctg ctggagcatt gcgcgcgctg ggccgaggag
      721 atgccgctgg cgctgattgc cgagggcatc ccgggcaatg ggtggcggat cttcccggag
      781 ggatgcgcgc cggaggcggc ccgcttctgc ctgatcgtcg atccaatcga tggcacgcgc
      841 ggcctgatgt atgacaagcg gagcgcctgg gtgctctgcg gggtggcgcc caaccgaggc
      901 gaggccacca cgctctcgga tatcgtcatc gcgatgcaga cggagatccc cacaacgcgc
      961 caatacctgg cggatcgcct ctgggcggtc cggagagagg gtgcccgcgc cgagcgaagc
     1021 aatctgctga cgggcgaatc gcgtgcctac acgccccaac cgagccgggc cgagagtctc
     1081 gcccacggct tcgccgggat caccaagttc ttccccgggg cgcgcgacct ggtggcatcc
     1141 ctcgaggaga ccctcttcga gcggatcctc ggcacgcccg cggatggcaa tcccctcgtc
     1201 ttcgacgacc agtacatctc caccggtggc cagctctacg agctgatcgt cgggcacgac
     1261 cggttcaacg ccgaccttcg ccctctggcg cttgccgcga agtatcccgc tgcgagccgg
     1321 caccgcctct gctgccatcc ttacgacttc tgcacggagc tgatcgcacg cgaggcgggc
     1381 gtgatcgtca ctgatccctg gggggcggag gtcacctgcc ctctggatat ccgggtcgat
     1441 ctcgcgtggg ttggctatgc caatcggaag atacaggcgc aggtggggcc ggtgctacag
     1501 gagctgcttc ggaagtggga gggtggcgtg ccgggatagt ggagaggcgg gagaggcgtt
     1561 ccccacccct ccacgatccc gaaccgggtt ctattgcagg aacagatcgt cgaggcgagc
     1621 ggtgaggctc accgcgggat agcgcatatc catcgcgccg gcgctcagcg tgaagcggtc
     1681 gcgccactgg tactgggcgc ccacatggaa gaggtagtcg ccccgcccgt aggagaggtt
     1741 cttgccccac tccacgagga gccgggtatt gcgggccacg ccccactcga tccccgcgaa
     1801 ggggctgtcg ccatagatcc ccgagccaat cccaagatgg ccccagagcg gatggcgggc
     1861 ttcgccggtg aactcgtcgc gtggcgacca gagcagcttg ctcgccacga gatacaccga
     1921 ggggtcactc accacctcgc cggtgatatc agtcacgccg atcgcaacag cgggaagcct
     1981 gtctttttcg gggagcgcca gccacttgcc atgcaccacg atgtctcgcg ccttccgcga
     2041 gctgtaggcc acatgggaaa gccccacctc cgccgtgccg aggagcccga agttgacaga
     2101 gtaccactcg ttgtccttcg tctcatcgtt gcggtcaatc cagacggcgc tcacggttgt
     2161 ctgcagcctt ccctcgggaa gtacgcgggc atgcggcacc accagcccgc ccggcgtgcc
     2221 cagcatcgag gtatccgccc acccgccggc accggcgaga gaaaggagaa gagtcatcac
     2281 gagagccttg cgtttcatca tctatttcgt cctccgtcaa cgcgcgtccc gcgcggccca
     2341 gcccgcttta agacacgcac gggtctcccc tattcgcgtc tccactatcg ctcatccgca
     2401 ccgggaagca ccgcccgcac cgtgaagtcg cccgtagcag ctccacgtga aggcgggtag
     2461 gcacagaacc aggccaggcc ggccaggacc accagggtca ctacagccag caggagcaag
     2521 aaaccacggc actcgcctgg tccggcgcac tcctcgaaac gctccccatg ctctagcgac
     2581 acgtctccac ctgatccgtt cgcattccga ttactgtagc ttcaccttca ccgaggctat
     2641 ttcggaccga tgccccaccg gatcaatcgc cacagcctga atcgtcacaa tctctccctc
     2701 tcgccggggt cgaggaacct gaagcttgcc cgtgacgaag ccgccgtcgc tatccacctt
     2761 cacctcctgc gcggcgagca cgtcgtgatc gctccacagg cccagagtgc gcgtcttgcg
     2821 caccacctgc acgcgcacgg tcactcccgg ttgggccttc ccatggacag tcagcgggcc
     2881 ctcgacgacc tcatcttccg aaggagcgct gatcgagggg gcgcgcgggg gcaccgtcag
     2941 gatggtgatc ggctccgagc actctcgcgt aaactgctcg cccgtctccg tgacgagttg
     3001 cgccacaatg cgcgcagcat aggccacgtc gcccttctcg gcggtgtagc ggccctggta
     3061 gatgccgggt gccgtctccg tcatggggat cccggtcttc atcgtcccga tgctgaaggt
     3121 ggcggtccca cgcggcttgc cggccatcgt gacggtgaag ctctggccca gacccaccgg
     3181 acgcgccgcg ttgtgggcca ccgaggcgat aggaacctcg g
//
LOCUS       DAOSCV010000501         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19249_length_3213_cov_4.046232, whole genome shotgun sequence.
ACCESSION   DAOSCV010000501 DAOSCV010000000
VERSION     DAOSCV010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19249_length_3213_cov_4.046232"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..1553
                     /locus_tag="PLY56_12240"
     CDS             45..1553
                     /locus_tag="PLY56_12240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ22296.1"
                     /translation="MAEKPSHRYRLAAIVWCALLAALLFQAGLVILHLFLDITFPYPL
                     DYGEGIALDRAIALANGESLYLPMDRPPYISWNFPPLYAWICGLGARWAGPGFAFGRL
                     VSAASILITAACVYLLARRHIEEDVPQASRIWGLVAALLYLGSTPVIVWGALMRVDSL
                     AIALGLSGYTAVVTGLSPVLAGLLFGAAILCKQSVVAGLLAAGLMLTFARRWRDLALV
                     LASCALLVGSVLAWQHLATDGGIWEHIILCNANAWRFDQLRWVAGPVVRDHSVLLLMA
                     VLGAVWPKAGRSRAPRNPGLYYLVSAALVSLLVGKVGSTINYLLDLTAATSIMATGFA
                     ARLDSALDHRIGGGIGAMVAPWLLCLHAWNSLAFPPIEKRVAMGAATAAVVRTLARAP
                     GPVIAEDPGLVPTAGKPLWLQPFEFTQMAEAGRWNQEPLLAALRQGEFSFIVLRFDPW
                     NSSHRDPEGTWAGGRFTDEMVAAMRDSYQIAERYYTWVILRPLERDGAAGTR"
     gene            complement(1631..2452)
                     /locus_tag="PLY56_12245"
     CDS             complement(1631..2452)
                     /locus_tag="PLY56_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOJ22297.1"
                     /translation="MRIYLMTDMEGVAGILDWENWCRPGAAYYDLGKELLTGEVNAAV
                     DGFFAGGATEVVVADGHGSGGINPLLLDRRAQLMRGWPAGYPLGIEESKYDAIAWVGQ
                     HAKAGTEKAHLAHTQSFSYLDLSINGISIGEFGQMVFCAAQYGVPAIFGAGDLAFTKE
                     AAELVPGIETVAVKRGLRSGAGEDLTAEAYGRYNTAAIHLAPEKAREEIRAGAFRAVQ
                     RFREAPFGLRTLKPPFERVAIFRGQDGAPKTISRETHPTDLCALMNMPFNPQPMN"
     gene            complement(2537..>3213)
                     /locus_tag="PLY56_12250"
     CDS             complement(2537..>3213)
                     /locus_tag="PLY56_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ22298.1"
                     /translation="IESPEPLIDERSLRYNFTNEGGVGGTIRFLKNIMGLWLVQECRR
                     AWLREGNDFSYAELTRLASQAPAFVSIVDPDDETFMAPDDMPDAICAFCRRTGQPEPE
                     TTGAVVRCCLESLALKYRWVVEKLEEIRGRRLETIHIVGGGSQNRLLNQFAADATGRP
                     VVAGPVEATAIGSVLIQAIARGHLGSLAEAREVVRASFEVETFEPKPSPGWDEAYQRY
                     LQVAAE"
BASE COUNT          495 a         1100 c         1039 g          579 t
ORIGIN      
        1 gccggccgcg atagagttcc accgagatgc accaggagga ctgtttggct gagaaaccat
       61 cccaccgata ccgactcgcc gcgatcgtct ggtgcgcgct gttggctgcc ctgcttttcc
      121 aggcaggatt ggtcatcctg cacctcttcc tggatatcac ctttccctac cccctcgact
      181 atggcgaggg gattgcactt gaccgcgcca ttgccctcgc gaacggcgaa tcgctctacc
      241 ttccgatgga tcgcccaccc tacatctcgt ggaacttccc gcccctctac gcctggatct
      301 gcggcctggg cgcccgctgg gcaggccccg gcttcgcttt cgggcggctg gtctccgcgg
      361 catccatcct gatcaccgcc gcctgtgttt acctgctggc gcgtcgccac atcgaggagg
      421 atgtccccca agcttcccgg atctggggcc tggtggccgc gcttctctac ttggggagca
      481 cgccggtgat cgtctggggg gcgctgatgc gcgtcgactc cctcgccatc gccctcggtc
      541 tgagcggcta cacggcggtg gtcacagggc tctcccccgt gctagccggc ctgctcttcg
      601 gcgcggcgat cctgtgcaag caatcggtag tcgcgggtct cctggctgcc gggctgatgc
      661 tgacgtttgc ccgtcgctgg agagatttgg ccctcgttct cgccagctgc gcgctgctcg
      721 tcggatcggt actcgcctgg cagcatctcg ccacggatgg gggaatctgg gagcacatca
      781 ttctctgcaa tgccaacgct tggcgcttcg atcaactgcg ttgggtagcc ggacctgttg
      841 tgagagacca ctcggtgctc ctcctgatgg cagtactcgg agccgtgtgg ccgaaggcgg
      901 gccgatcgcg cgcgccgcgg aacccggggc tctactacct ggtgagtgcc gctctggtct
      961 cccttctcgt cggaaaggtc ggttcgacca taaactacct gctcgacctg acggcggcca
     1021 ccagcatcat ggcgaccggc ttcgccgcac gcctagattc ggcgctcgat caccgcatcg
     1081 gcggagggat tggcgccatg gttgcgccgt ggctcctttg cctgcatgcg tggaacagcc
     1141 ttgcctttcc ccccatcgaa aagcgggtgg cgatgggcgc ggcgaccgcc gcggttgtac
     1201 gcacgctggc gcgggctccc ggacccgtga tcgctgagga tccgggcctg gtcccaaccg
     1261 ccgggaagcc gctttggctg caaccgttcg agttcaccca gatggccgag gcggggcgat
     1321 ggaaccagga gcccctcctc gcggcgctgc gccaggggga gttctccttc atcgtcctcc
     1381 gctttgatcc ctggaactca tcccatcgcg atccggaggg cacgtgggca ggtgggcggt
     1441 tcaccgacga gatggtcgcc gcgatgcgtg attcctacca gatcgcggag cggtactaca
     1501 catgggtcat cctgcggccc ctagagaggg acggggccgc aggcactcgg tgatagggcg
     1561 cgggaaagcg agtccgcttc catcgaaagc gcggtaaagc cgcccggccc ccgggcatct
     1621 cctcgcgcgt ctagttcatg ggctgcgggt tgaacggcat gttcatcagc gcgcagagat
     1681 cggtggggtg cgtctcgcgg gagatcgtct tcggcgcgcc atcctggcct cggaagatcg
     1741 cgacgcgctc gaagggcggc ttcagggtgc ggaggccaaa gggcgcctct cggaaacgct
     1801 ggacggcgcg gaaggcgccc gcgcggatct cctcgcgcgc cttctcgggc gctaggtgga
     1861 tcgccgcggt gttgtagcgc ccgtaagcct cggccgtcag atcctcgccc gcgccggaac
     1921 gcaggccgcg cttcacggcc acggtctcga tcccgggaac gagctcagcg gcctctttgg
     1981 tgaaggccag gtcgccggcg ccgaagatcg ccggcacgcc gtactgggcg gcgcagaaca
     2041 ccatctggcc aaactcgccg atggagatgc cattgatcga gaggtccagg tagctgaagc
     2101 tctgagtatg cgccaggtgc gccttctcag tgcccgcttt cgcgtgctgg cccacccagg
     2161 cgatcgcgtc gtatttggac tcctcgatcc cgagcgggta gccggctggc cagccgcgca
     2221 tcagctgggc ccgccggtcg agtagcagcg ggttgatccc gccggagccg tgcccgtcgg
     2281 cgacgaccac ctcggtggcg ccgccagcga agaacccgtc caccgccgcg ttcacctcgc
     2341 cggtgaggag ctccttgccc aggtcataat aggccgcccc gggccggcac cagttctccc
     2401 aatcgaggat accggcgacg ccctccatat cggtcatcag gtagatgcgc attggaacct
     2461 ccgataaaga tccgccgaga gcgcggcagt atccgccagg caggcgcgcc cgctcccgag
     2521 tctgcgcgac accgtcttac tcggcagcaa cctgaaggta gcgctgatac gcctcgtccc
     2581 acccagggga gggtttcggc tcgaaggtct ccacctcgaa cgaggcgcgc accacctccc
     2641 gggcttcggc aagcgagcca aggtggccgc gagcaatcgc ctggatcagg acgctgccga
     2701 ttgcggtcgc ctctaccggt ccggcaacca cgggccggcc cgtcgcatcc gcggcgaact
     2761 ggttgagcag gcggttctgc gaaccaccac cgacaatgtg gatcgtctcc agacgccgcc
     2821 cgcgaatctc ctccagtttc tccacaaccc agcggtactt gagtgccagg ctctcgaggc
     2881 agcagcggac cacggcgccg gttgtttccg gctcgggctg gccggtgcgc ctgcagaaag
     2941 cacagatcgc atccggcatg tcatccggcg ccatgaaggt ttcgtcatcc gggtccacga
     3001 tggagacgaa agcgggcgcc tgggaggcaa ggcgggtcag ctcggcgtac gagaagtcat
     3061 tcccttcgcg cagccaggcg cgccggcact cctgcaccag ccacaggccc atgatgttct
     3121 tcaggaagcg gatggtgcct ccaacgcccc cctcgttcgt gaagttgtag cgcaacgagc
     3181 gctcgtcaat cagcggctcc gggctctcga tcc
//
LOCUS       DAOSCV010000502         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19260_length_3211_cov_3.890051, whole genome shotgun sequence.
ACCESSION   DAOSCV010000502 DAOSCV010000000
VERSION     DAOSCV010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_3211_cov_3.890051"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3211
                     /locus_tag="PLY56_12255"
     CDS             <1..>3211
                     /locus_tag="PLY56_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22299.1"
                     /translation="RGAKVAALAPGRKLPKLTPDTLFREWTRAQVERWEKEHPPLPEE
                     EAYQRYAATSPTDEELTTDFPDHIAPFGLVASGKPNPEDASAVLIRYCPFCQSRALSI
                     QYDSANPYHAVTVCCKTHLYGRDYPADYPLKPNSVARFHHLDDTVKEVPCTLYRDRDG
                     VVWQLFIGTLFSHRRWLEVGSGLVVTFYKQFRETANPVYAHKIAVILDKVADTYYGLP
                     LCSGNELAKGKDGKPLTRAEWEAVPRPAIFEVSYLGPWNRRTPIGNRGWLNMSREHIW
                     VEPFARVRHHPAFRAYSKKRYGDPEALDRKIMQKLMRELALMFKSVFSQKLITNYQEA
                     NYTDLFLLGVLLQDRELIDFAGPNQELTLYNHHYHDGVNGEGAQNYMAMLNSYYYPYL
                     RNPEGWLELYPDFLKDHPFFGPASTELNRLATVRGIHLEFADQHEYAWRPPLVDPEKV
                     RANEAVPSRNWPGFGVGLLRVGGSGHRMEVSLHYSRATLHTASDALGISCWVDGVPVM
                     RPGGYAAHWSNAPLDAKRPEIQALHQMGYPREIAEAGRGFDGWSWIWTHSALAQNTVA
                     VDEKATSGGWRDDRGFGEVITYKGGEEPGQIGARFQVMDVRDLYSFERAGVPVSRFRR
                     TLLGVEAPDGRAYVLDCLRLSGGQRHALYQSAWADRAEARLPAATKRYPDLTHVWWGG
                     KLPEDTSQTRNYHQVRNVEALAPPAGPWELTWKTDIAAYAPRDPGGKPFVRPLPDDVG
                     RVRLRLIGVPQPEGAPRLLNAKGPWIAWLRQPLPGGTRVDGYVAFLDAIDFLIEQRVA
                     ADPKQPLESRFVHVLEGFREGEKTAIRSVEALPVQTPGALAVKLELASGYTDTVLFQE
                     EPGTLRLPDGIETDARYALLRRDRAGRIVEAHVVRGTFLRGEGFRYQGPAEFRGTIVD
                     LIGDLTGTRHESALIVRPEQPWPEGDTLAGRQLLIRLKRANGRENNEAFTVSRVTPLP
                     GGLLRVDLDGYPPFAAGWHQVAFLDEERPNVLKTHRPMVKFGNLPWYEGCKAWFPERG
                     KTYVIKDTEAVGGGFGGCVLELEDSANLAADGIRPGDWYV"
BASE COUNT          549 a         1099 c         1050 g          513 t
ORIGIN      
        1 accgaggcgc gaaagtagcg gcgctcgcac cgggccggaa gctgcccaag ctcactcccg
       61 acacgctctt ccgggagtgg acgcgcgccc aggtggagcg ctgggaaaag gagcaccctc
      121 ccctcccaga agaggaggcg taccagcgct acgcggcgac gtcacccacc gatgaggagc
      181 taacgaccga cttccccgac cacatcgcgc ctttcgggct cgtggcttcc ggcaagccga
      241 acccggaaga tgcctccgcc gtgctcatcc gctattgccc cttctgccag tcgcgcgcgc
      301 tctccatcca gtacgatagc gccaacccat atcacgccgt caccgtctgc tgcaagaccc
      361 acctctacgg gcgcgactat cccgcggatt atcccttgaa gcccaactcc gtggcccgct
      421 tccaccacct ggatgacacg gtgaaggagg tgccgtgcac gctctatcgt gaccgcgacg
      481 gcgtggtgtg gcagctgttc atcggcaccc tcttctccca cagacgctgg ctcgaggtcg
      541 gcagcggcct ggtagtgacg ttttacaagc agttccgaga gacggcgaac ccggtctatg
      601 cacacaagat cgcggtaatc ctcgacaagg tggccgatac ctactacggc ctgccccttt
      661 gttccgggaa cgagctggcc aaagggaaag atggcaagcc gctcacgcgc gccgaatggg
      721 aggccgtgcc gcgcccggcg atcttcgagg tttcctatct gggcccctgg aaccggcgca
      781 cgccgatagg caaccggggc tggctcaaca tgagccggga gcacatctgg gtggagccgt
      841 tcgcgcgcgt ccgccatcat cccgcgttcc gcgcctactc aaagaagcgg tatggcgatc
      901 ccgaggcgct cgaccgcaag atcatgcaaa agctgatgcg cgagctggcg ctcatgttca
      961 agagcgtctt ctcccagaag ctgatcacca actaccagga agcgaactat accgatctct
     1021 tcctcttggg cgtcctgctc caggaccggg agctgatcga cttcgccggg cccaatcagg
     1081 agctgacgct ctataaccac cactaccatg atggcgtcaa cggcgagggt gcgcagaact
     1141 acatggcgat gctcaacagc tactactatc cctacctgcg caaccccgag ggatggctgg
     1201 agctatatcc cgatttcctc aaggaccatc ccttcttcgg ccccgcgagc acggagctca
     1261 accggcttgc caccgtgcgt ggcatccacc tggagttcgc cgaccagcac gagtacgcgt
     1321 ggcgcccgcc gctcgtcgat cccgagaagg tgcgcgccaa cgaggcggtt ccaagccgca
     1381 actggcccgg cttcggcgtg ggattgctcc gcgtcggcgg ctccggccac cggatggagg
     1441 tctccctcca ctactctcgc gcgacgctcc acaccgcctc ggacgccctt ggcatctcct
     1501 gctgggtaga cggcgttccg gtaatgcgcc cgggtggtta cgccgctcac tggagcaacg
     1561 ccccgctcga cgccaagcgg cctgagatcc aggcgctcca ccagatgggc tatccccgag
     1621 agatcgcgga ggccgggcgc ggcttcgacg gctggtcgtg gatctggacc cacagcgccc
     1681 tcgcacaaaa caccgtcgcc gtggatgaga aggccacgag cggtggatgg cgggatgacc
     1741 gcggctttgg cgaggtgatc acctataagg gcggcgagga gcccggccag atcggcgccc
     1801 ggttccaggt gatggatgtg cgcgacctgt actccttcga gcgcgccggc gtgcccgtct
     1861 cccggttccg caggaccctc ctcggcgtgg aagccccgga cggccgggcg tacgtcctgg
     1921 actgcctgcg gctttcgggc ggccagcggc atgctctgta tcagagcgcg tgggccgacc
     1981 gcgcggaggc gcgcctgccc gccgcgacga agcgttaccc tgatctcacg cacgtctggt
     2041 ggggcggaaa gctccccgag gacacgtcgc agacgcgcaa ctaccaccag gtgcgcaacg
     2101 tcgaggcgct cgctccaccg gcagggccct gggagttaac ctggaagacc gacatcgccg
     2161 cgtatgcccc tcgcgacccc gggggaaagc cgttcgtccg cccgctgccg gacgacgtcg
     2221 gccgggtgcg cctccggctc atcggagtgc cgcagccgga gggcgccccg cgccttttga
     2281 acgccaaggg gccgtggatc gcctggttgc ggcagccgct ccccggcggt acccgggtgg
     2341 atggttacgt cgccttcctc gacgcgattg atttcctcat cgagcagcgc gtcgctgcgg
     2401 atccgaagca gccgctggag agccgcttcg tccacgtgct ggagggcttc cgcgagggcg
     2461 agaaaacggc gatccgctcc gtcgaggcgc tcccggtcca gaccccaggc gcgctggccg
     2521 tgaagctaga gctggcgagc ggctacaccg acaccgtgtt gttccaggag gagccgggca
     2581 cactccgcct gccggatggg atcgagacag acgcgcgcta cgccctgctg cgccgcgacc
     2641 gcgccggccg gatcgtcgag gcgcacgtgg tccgcggcac cttcctgcgc ggagagggat
     2701 tccggtatca ggggccggct gagtttcgtg gcacgatagt cgatttgata ggcgacttga
     2761 cgggcacccg gcacgagagc gcgctgatcg tccgccctga gcagccttgg cccgagggcg
     2821 acacactcgc cgggcggcag ctgctaattc ggctgaagcg agccaatggc cgggagaaca
     2881 acgaagcctt caccgtgagc agggtcacgc cgctgccggg cgggctgctc cgggtggatc
     2941 tggacggcta cccgccgttt gccgccggct ggcaccaggt cgcgttcctc gatgaggaac
     3001 gtccgaacgt gctcaagaca caccgcccga tggtcaagtt cgggaatctc ccctggtacg
     3061 aggggtgcaa ggcgtggttc cccgagcggg gcaagaccta cgtgatcaag gataccgagg
     3121 cggtgggcgg cggttttggc gggtgcgtgc tggagctgga ggactccgcg aacctcgccg
     3181 ccgacgggat ccggccgggc gactggtatg t
//
LOCUS       DAOSCV010000503         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19290_length_3208_cov_3.073898, whole genome shotgun sequence.
ACCESSION   DAOSCV010000503 DAOSCV010000000
VERSION     DAOSCV010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19290_length_3208_cov_3.073898"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="PLY56_12260"
     CDS             <1..947
                     /locus_tag="PLY56_12260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22300.1"
                     /translation="TPAELAQLFNTAFGEYEGVVYFTPETMAWYLARPGLGSEHTFVA
                     LAGDQLIGGVMVTCAPVVLGGEPISCGIIDSVMTLPTWRRRGVATRLMEHALAWMREA
                     GLESSLLFTVPHSNGYRIYSRLGYIERATVSYYFALPAPQGEADPRLRAAVPGEEERV
                     RVLLNTWLRESDGFVPVDPVLWRWRREERPHELPCSLFVLEDAGQIRATGAVCTTRVQ
                     LDGRPLDLVVLTDIAGDSTDAREAMVRALLRRVPPGFGVGMLAGSYDPVLLALGEKLG
                     MSCQNEVAMVIPFGLRAEELLDRQPAGWYTLAESLIGV"
     gene            965..2356
                     /locus_tag="PLY56_12265"
     CDS             965..2356
                     /locus_tag="PLY56_12265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ22301.1"
                     /translation="MKISFLGAARTVTGSMHLLEVNGARILLDCGLYQGRRAEANERN
                     RHLPRPALDADCLILSHAHIDHSGNIPGLCRQGFSGPIYATRATQDLCAVMLADSAHI
                     QEQDAAYLNKKLQRRGEPLVEPIYTTLNAQQAMSQFVGVDYGVPFEPVPGVSARFLDA
                     GHILGSAAIVLDVEEGGKSHRFCFTGDVGRPNTPIIRDPEPLEGVEWLVTESTYGNRL
                     HDPIGDAEAQLADTVNETARRGGKVIIPAFSVGRTQELVYALARLRAENRIPEIPIFV
                     DSPLSTNVTGVFRKHPECFDQEMNALLAKQDDPFGFSKLAYTRSVADSKQINFLADPC
                     IIISASGMCETGRILHHLSNNIGDPKNTVLIVGFQAANTLGRRLVEKWPQVRIFGEEH
                     RVRAQVRVINALSAHADRDELLRYIGATQETLRKVFVVHGEESQSLALADTLFNLGIP
                     EVAVPALGETHTL"
     gene            2437..>3208
                     /locus_tag="PLY56_12270"
     CDS             2437..>3208
                     /locus_tag="PLY56_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOJ22302.1"
                     /translation="MARLWVWASLAVALLATTGCGNGDGAERRSQRAWTFMVYLDGDN
                     DLEEAGVWDVNEMERVGSTDAVSIVVQFDRGPQVNGAGDWSTARRFLIAPDRDLQSIT
                     SPLLMDLGEVDMASTQALRGFVSWATSEFPADHYALVLWNHGAGWRSRSPGTGAAGGS
                     RGIHFDDTSGTAMTMDGLRAALSTLPARIDLVAIDASLMGMLEVCYEIHDQTDYIVAS
                     EESPPGTGYPYDVILSRLVADPEMTPADLGRVIVDAHVE"
BASE COUNT          519 a         1033 c         1055 g          601 t
ORIGIN      
        1 ggacgcccgc agagcttgcc cagctgttca acaccgcctt tggcgagtat gaaggcgtcg
       61 tctatttcac gccagagacg atggcgtggt atctggcgcg ccccggtctc ggaagcgaac
      121 acacctttgt cgcgctggca ggtgatcagc tcatcggcgg agtgatggtc acctgcgccc
      181 cggtggtttt ggggggcgag ccgatctctt gcgggatcat cgattcggtg atgactttgc
      241 caacctggcg ccggcgagga gtcgccacgc gcctcatgga gcatgcgctc gcctggatgc
      301 gcgaagcggg gctggagtcc agcctgcttt tcaccgtgcc ccactccaac ggttaccgca
      361 tctattcccg tctcggctat atcgagcgtg ccacggtcag ctactacttc gcccttccgg
      421 cgccacaagg cgaggccgac ccacgcctgc gcgccgccgt gcccggcgag gaagagcgcg
      481 tgcgcgttct gctcaacacc tggcttcgcg agagcgatgg ctttgtcccg gtggatccgg
      541 tgctctggcg atggcgtcgc gaggagcgcc cgcacgagct accctgctcc ctcttcgtcc
      601 tggaagatgc tggccagatc cgggcgacgg gagcggtttg caccacccgg gttcagctcg
      661 atggccgccc gctggatctc gtggtgctca ccgacattgc cggagatagc acagatgcgc
      721 gcgaggcgat ggttcgtgcg ctcctgcgtc gcgtgccgcc cgggtttggc gtgggaatgc
      781 tcgccggctc gtacgatccg gtgttgcttg ccctgggaga gaagctgggc atgtcttgtc
      841 agaatgaggt cgcgatggtg attccttttg ggctgcgtgc cgaggagctg ctggaccgcc
      901 agccggcggg gtggtacacg ctcgccgagt cgctgatcgg cgtctgacgt ggagccgcta
      961 agtcatgaag atctcctttc tgggcgcagc ccgcacggtg actgggtcga tgcacctcct
     1021 ggaggtgaat ggcgcgcgca tcctcctgga ttgcggcctc taccagggcc gccgggcgga
     1081 ggcaaatgag cgtaaccggc atctcccccg gcctgccctg gacgcggact gcctcatcct
     1141 ctcgcacgcg cacattgatc acagcgggaa tatccccggc ctctgccgcc agggcttcag
     1201 cgggccgatc tacgccaccc gcgccaccca ggatctgtgc gccgtgatgc tcgcggactc
     1261 tgcgcatatc caggagcagg acgcggccta cctcaacaag aagctccagc gccgcggcga
     1321 gccactggtt gagccgatct acaccacgct caatgcccag caggcgatga gccagttcgt
     1381 cggagtggat tacggcgttc ctttcgaacc cgtaccaggg gtttcggcgc gtttcctcga
     1441 tgccgggcat attctgggat cggctgccat cgtgctcgat gtggaggaag gcggcaagag
     1501 ccaccgcttc tgcttcaccg gcgacgtagg ccggcccaac acgccgatca ttcgcgatcc
     1561 ggagccactt gaaggcgtcg aatggctggt cacagagagc acctacggca accgcctcca
     1621 cgatccgatc ggtgatgccg aggcgcaact cgccgatacg gtgaacgaga cggcccggcg
     1681 tggcgggaag gtgatcatcc ctgcgttttc cgtcggccgg acccaggagc tggtgtatgc
     1741 cctggctcgc ttgcgcgccg agaaccgcat ccccgagatc ccgatctttg tcgacagccc
     1801 gctctcgacc aacgtgaccg gggtctttcg caagcatcca gagtgcttcg atcaggagat
     1861 gaatgcgctc cttgcgaagc aggatgaccc gtttggcttc agcaagctcg cctacactcg
     1921 ctcggtggcg gattcaaagc agatcaactt cctggcagat ccgtgcatca tcatctctgc
     1981 ctcggggatg tgcgagactg ggcgcatcct ccatcacctc tcgaacaaca ttggcgatcc
     2041 aaagaacacc gtgctcatcg tcggcttcca ggcggcgaat acgctgggtc ggcgcctggt
     2101 ggagaagtgg ccgcaggtgc ggatcttcgg tgaggagcac cgcgtgcgcg cgcaggtgcg
     2161 cgtgatcaac gccctgagcg cccatgccga ccgcgacgag ttgctccgct atatcggcgc
     2221 cacccaggag acgctgcgca aagtgttcgt ggtccacggc gaggagagcc aatcgctcgc
     2281 cctggcggat actctcttca accttggaat cccagaggtc gccgtacccg cgctgggaga
     2341 gacgcacact ctttgaagaa cacctttctg gtatgagact tgccttcccg cccgtcgggt
     2401 ggcccgcagg cttggggcca ctccgagggg tggggaatgg ccaggctctg ggtgtgggcg
     2461 tctctcgcgg tggcgcttct tgccacgacc gggtgcggca acggcgacgg cgccgaacgc
     2521 cggtcgcagc gcgcatggac cttcatggtc tatctcgatg gcgataacga tctggaggag
     2581 gcgggcgtct gggacgtgaa cgagatggag cgcgtgggct ccacggacgc ggtctccatc
     2641 gtggtgcagt ttgaccgcgg cccgcaggtg aatggcgccg gtgattggtc cactgcgcgc
     2701 cgtttcctga tcgcgccgga ccgcgacctg cagagcatca cctcgcccct gctcatggat
     2761 ctgggcgagg tggatatggc cagcacgcag gccctgcgcg gttttgtctc ctgggcgacg
     2821 tccgaattcc ccgcggacca ctatgccctc gtcctgtgga accacggggc cggctggcgg
     2881 tcgcgctcgc ctggcacagg ggcggctggc ggatcgcgcg ggattcactt cgatgacacg
     2941 agcggcacgg cgatgacgat ggatgggctg cgtgccgcgc tgagcaccct ccccgcgcgg
     3001 atcgacctcg ttgctatcga tgcgagcctc atggggatgc tggaggtgtg ctacgagatc
     3061 cacgaccaga ccgactacat tgtggcctcg gaagagtcgc cccccggaac cggctatccc
     3121 tatgacgtca tcctctcgcg cctggtggca gaccccgaga tgacccccgc cgatctggga
     3181 cgcgtgattg tcgatgcgca cgtcgagg
//
LOCUS       DAOSCV010000504         3205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19311_length_3205_cov_3.675873, whole genome shotgun sequence.
ACCESSION   DAOSCV010000504 DAOSCV010000000
VERSION     DAOSCV010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19311_length_3205_cov_3.675873"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLY56_12275"
     CDS             complement(<1..236)
                     /locus_tag="PLY56_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22303.1"
                     /translation="MLNDLSSQVFLNALLVLGLAAAVIIDLRSRRIPNLVTAKVGARD
                     SVSVFNANGEAHFVADVVGYYDSGGADDGDLFRGL"
     gene            complement(458..1123)
                     /locus_tag="PLY56_12280"
     CDS             complement(458..1123)
                     /locus_tag="PLY56_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22304.1"
                     /translation="MDRVRVLIADDHPLILEGLWSLLERQEGIEVVGKASDGPTAVKQ
                     ALELEPDVILMDIRMPGEDGVEATRRIRHASPHIRVIILTVYEDDASIFSAIKAGACG
                     YILKDVDTDQLIDSIRRAARGESLIHPAIAARVLDEFTRLTREQERMVPDLFQQLTKR
                     EVEILHLLAEGLSNKEIAFRLSISEKTVKNHLSSIFSKLQVNDRMQALIYAFEHNLIP
                     SNS"
     gene            complement(1116..1799)
                     /locus_tag="PLY56_12285"
     CDS             complement(1116..1799)
                     /locus_tag="PLY56_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ22305.1"
                     /translation="MDERLISLRCAEAREEERRRIAVDLHDGPIQQLANLILRLDMAQ
                     KVIPADAMEARTEMASLHQLAKSTLHDMRRFMFELRPSSLEEFSLLPVLRQYVQNYRA
                     QYHIPVGLHLSDSDWSLGRDIEVNLFRIIQEALTNVRKHANASQVDVTLSRQGPVVKA
                     TIRDNGQGFDVITTRTRAQHEKRLGLASMEDRARALNGTLQIESRDGHTEVCITVPLV
                     AQKTTADHG"
     gene            2278..2637
                     /locus_tag="PLY56_12290"
     CDS             2278..2637
                     /locus_tag="PLY56_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22306.1"
                     /translation="MARKEGKKEISVREAGRKGGETTAERHGREFYERIGRKGGPIGG
                     QRRKEELGHGGYEELGRKGGNVRKRQLGPEGYSELGKMGGNVRKQQLGHEGYEELGQK
                     GGQRVRELIEKGKQAES"
     gene            complement(2703..>3205)
                     /locus_tag="PLY56_12295"
     CDS             complement(2703..>3205)
                     /locus_tag="PLY56_12295"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ22307.1"
                     /translation="FGMAVYYLAPLLDRPISGTLMGLYTLGTGVYLLFFDSAGRSLRG
                     FFAAKRGLGALVAVAGVSLLIPRAQPAGIPFEAYSEERLQEALAEGRGVLIDFRAEWC
                     TACRELETKTFRDPKVVAATRDLLPLKVDMTRWNAAQTQRLKEEYGIVGLPTVVLVPP
                     ASGQGE"
BASE COUNT          622 a          949 c         1069 g          565 t
ORIGIN      
        1 agccctctga aaaggtcgcc gtcgtccgcc cctccgctgt cgtagtagcc gaccacgtcg
       61 gccacgaagt gcgcctcccc gttcgcgttg aacacgctga cgctgtcgcg ggcgccgacc
      121 ttcgccgtca cgaggttcgg gatgcggcgt gagcgcaaat cgatgatgac cgccgcggcc
      181 aacccgagaa ccagaagggc attgaggaac acctgcgatg acaagtcgtt cagcatctct
      241 ccctaaccgc gcgcggagtc atccgccgcc cggatccaaa ggatcggcct gcatagatgg
      301 ccgatccctg agaattcacc actactatta tgcctgagac cccgcgtctc tgccatatca
      361 cgatggtcct aacggtggta ggctaaaagt cctacgcacc aacaaccggc tggtatcatg
      421 ggataccccc atcaggtgac ttgggcgcgc gagggactca gctgttggag ggaatcaggt
      481 tgtgctcgaa ggcatagata agcgcctgca tgcgatcatt gacttggagc ttgctgaaga
      541 tgctgctgag gtggttcttg accgtcttct cgctaatcga gagccggaag gcgatctcct
      601 tgttgctgag gccctcggcc agcaggtgaa ggatttcgac ctcccgcttc gtgagttgct
      661 ggaagagatc cggcaccatg cgctcttgct cccgggtcag gcgcgtgaat tcatccagca
      721 cccgggcggc gatggccgga tggatgagtg attcgccccg ggcagcgcgc cggatcgagt
      781 cgatgagctg gtcggtatct acatccttaa ggatatagcc acatgcgccc gccttgatcg
      841 cgctgaagat cgatgcgtca tcctcgtaca cggtgaggat gatcacgcgg atatgcgggc
      901 tggcatgccg gattcggcgc gtcgcctcga cgccatcctc gcctggcatt cggatatcca
      961 tgaggataac gtccggttcc agttcgagtg cctgcttcac ggcggttggc ccatccgagg
     1021 ccttgccaac aacttcgatc ccctcctggc gttccaggag gctccacaga ccctccagga
     1081 tcagagggtg atcatcggcg atcagaacgc gaactctatc catggtcagc cgtggtcttc
     1141 tgggcgacaa gtggaacggt gatgcacact tccgtgtgac catcacggct ctcgatctgc
     1201 agcgtgccat tcagagcacg cgcacgatcc tccatgctgg cgaggcccag gcgcttctcg
     1261 tgttgggcgc gggtccgcgt ggtaatcaca tcaaatccct ggccattatc ccggatcgtt
     1321 gccttgacca ccgggccctg gcgggaaagc gtcacatcca cctgcgaggc attggcatgc
     1381 ttgcgcacat tggtgagcgc ttcctggatg attcggaaga ggttgacctc aatatcgcgc
     1441 ccaagagacc aatcgctgtc tgagaggtgg agcccgacgg ggatgtggta ctgggcgcgg
     1501 taattctgca cgtactggcg gaggacagga agcagcgaga actcttccag ggaggagggg
     1561 cgcagctcga acatgaagcg gcgcatatcg tgcagcgtgc tcttggcgag ttggtgcagc
     1621 gaggccatct ccgttcgcgc ctccatggca tcggccggga tcaccttttg cgccatatcc
     1681 agacggagga tgaggtttgc cagttgctgg attggcccgt cgtggagatc cactgctatt
     1741 cgccggcgct cctcctcgcg agcctcggcg cagcgcagcg agatcagccg ttcatccata
     1801 accccagtct cgctcgtggg agaagctgtt gcggcggagg cagcaccaag accatcgcca
     1861 cgcgggcgga ggcgtcgaat aaactcgctg acaaaaccca gggacatcga gaggtatcct
     1921 cccacggctt ggattgcttc ccgactcgct cgttgtagaa cgcgtgaacg gcaggagaca
     1981 atacgatatg gccttctaac ccaactgaca gtatagcaca catccgttag gatgtaaaga
     2041 agcatgccga gggtgttctg gctcctccat ctcggcgagt agcgatgcgg atggcgcgtg
     2101 aggaggcggg cggcgtggtg ccagcgaggg gctggcgctg caggcgcgcc gggcaggttt
     2161 ggcagccgga gcatccgggt aagcaggtca caagtgagct cagggatcgg cttgtctggc
     2221 ttggggcgat ggtgtgctgc gcaggcagga ggcggggcaa ggtgaggagg tcgcgaggtg
     2281 gccaggaaag agggaaagaa ggagatcagc gtccgcgagg cgggacgcaa gggtggcgag
     2341 acaaccgccg agcgccacgg ccgggagttt tatgagagaa ttgggcgcaa gggcggcccg
     2401 attggggggc agcgccgtaa ggaggagttg ggccacgggg gctatgagga gctggggcgc
     2461 aagggcggga acgtgcgcaa gcggcagctt ggccccgaag gctattcgga gctgggcaag
     2521 atgggcggga atgtgcgcaa gcagcaactg ggccacgagg gatacgagga actgggccag
     2581 aagggcggcc agcgcgtccg cgagctgatc gaaaagggga agcaggccga gagctgagcg
     2641 ccgcattcgc gcgcggggcc ggggagggag accgcgctct tcctccctag ccccctgcca
     2701 tctcactcgc cctggccaga ggccggtggc acgagtacca ccgttggcag cccgacgatc
     2761 ccatactctt ccttcagtcg ctgggtctgg gccgcgttcc agcgcgtcat atccaccttc
     2821 aaagggagca gatcgcgcgt cgcggcgacc accttcgggt cgcgaaacgt cttcgtctcc
     2881 agctcgcggc atgccgtgca ccactccgca cggaagtcga tgagcacacc gcggccctcg
     2941 gccagcgcct cctgcaggcg ctcctcgcta tacgcctcga agggaatccc ggctggctgc
     3001 gcgcgtggga tgagcagcga tacgccggcc acggctacga gtgctcccag gccgcgcttg
     3061 gcggcgaaaa agccccgcag gcttcgcccg gcggaatcga agaagagaag gtagacgccg
     3121 gtgcccaggg tgtagagccc catgagggtg cccgagatcg gccggtcgag cagcggagcg
     3181 aggtagtaga ctgccatccc gaaca
//
LOCUS       DAOSCV010000505         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19432_length_3191_cov_2.617985, whole genome shotgun sequence.
ACCESSION   DAOSCV010000505 DAOSCV010000000
VERSION     DAOSCV010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19432_length_3191_cov_2.617985"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLY56_12300"
     CDS             <1..434
                     /locus_tag="PLY56_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HOJ22308.1"
                     /translation="GETAAVERAGALAKERGARRVLPLNVSGAFHSPLMQPAADRLAE
                     VLNTTSLSDARIPVVANVHGEPVQTASSIRTALLQQITGSVFWEDSVRRMAADGVTTF
                     VEVGPGQVLSGLVRRIAPETRTLQAGDVDSIAATAEELRKA"
     gene            546..1292
                     /gene="fabG"
                     /locus_tag="PLY56_12305"
     CDS             546..1292
                     /gene="fabG"
                     /locus_tag="PLY56_12305"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOJ22309.1"
                     /translation="MRFKDRVVLVTGGGGGIGRTIALLFAGEGAHIVIGDLNIAGGEE
                     TVEMIRQKGGQAVFVEANVTRFEDAEKMVAQAVENWGRLDVLINNAGVTRDGLLLRMS
                     EEDWDLVLDINLKGAFLCTKAAIKVMLKQRSGAIVNIASVVGRMGQAGQANYAASKGG
                     LIALTKSVAKEVAGRNIRVNAVAPGFIETQMTHVLSDEVKKAWLERIPLNRAGTMEDV
                     AKVVAFLASDDASYLTGQTIGIDGGMLMSE"
     gene            1530..1769
                     /locus_tag="PLY56_12310"
     CDS             1530..1769
                     /locus_tag="PLY56_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ22310.1"
                     /translation="MDQSVFERVRKVIVEQLKVSEDEVTPAASFDDLGADSLAKVEMV
                     MAFEEEFSVEIPDEDAEKINTVGQAVSYIESKSAE"
     gene            1779..3017
                     /gene="fabF"
                     /locus_tag="PLY56_12315"
     CDS             1779..3017
                     /gene="fabF"
                     /locus_tag="PLY56_12315"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOJ22311.1"
                     /translation="MSRRRVVVTGLGAITPLGNTVAAYWEGLKAGRSGAGPITRFDAS
                     PFTTRIACEVKGFNPEEYIDPREVRHIDRFAQFAIAAAKQAVADSGLEITPENAERIG
                     VVIGSGIGGIETWEKQHAIYLNRGPGRISPYFVPMMICDMAAGHVSITLGCKGPNLAT
                     VTACATGTHAIGDAASIIRRGDADVMIAGGTEAAITPMGIGGFCAARALSTRNDEPER
                     ASRPFDKDRDGFVPGEGVGVLVLESEEHARARGAMIYAEVAGYGMTGDAHHITAPAPG
                     GEGAARAMLLALKDAGMAPSDVDYINAHGTSTQANDVAETAAIKRAYGEHARKLMVSS
                     TKSMTGHLLGAAGGIEAIACALAVRYGVVPPTINYETPDPECDLDYVPNTAREVRVRA
                     AMSNSLGFGGHNACLIFRAF"
BASE COUNT          583 a          975 c         1103 g          530 t
ORIGIN      
        1 cgggggagac ggccgccgtg gagcgcgccg gagccctggc caaggagcgc ggtgcccgcc
       61 gcgtgcttcc gctcaacgtc agcggtgctt tccactcgcc gctgatgcag ccggcagccg
      121 accggctggc cgaggtgctc aacaccacgt ccctttcgga cgcacgcata ccggtcgtgg
      181 ccaatgtcca cggcgagccg gttcaaaccg cctccagcat tcgaaccgcc ttgctgcaac
      241 agataaccgg cagcgtgttc tgggaggact ccgtgcgccg aatggcggcg gatggagtca
      301 ccaccttcgt ggaggtaggc ccgggccagg tgctctccgg cctggtgcgt cgcatcgcgc
      361 ctgagacccg cacgctgcag gctggcgatg tggacagcat cgccgccact gcggaggagc
      421 tgcgcaaggc gtagcgcgtg agatatcaga tgtgaggtgt ggatggacta cggggaagag
      481 cccccgctct ctttccctgc gtttccccct acacgcatca cggagtttct ggaaggatgg
      541 caggaatgcg cttcaaagat cgggttgtgc tcgtcaccgg tggcggtggc ggcatcggcc
      601 gaacgatagc ccttctcttc gcgggcgagg gcgcccacat cgtcatcggc gatttgaaca
      661 tcgctggcgg cgaggagacc gtcgagatga tccgtcagaa gggcggccag gcggtctttg
      721 tcgaggcgaa cgtcacccgg ttcgaggacg ccgagaagat ggtcgctcag gcggttgaaa
      781 actggggccg cctggatgtg ctcatcaaca acgccggtgt cacccgcgat ggtctgctcc
      841 tgcgcatgag tgaagaggac tgggatctcg tgctggatat caacctgaag ggcgcattcc
      901 tgtgcaccaa ggcagccatc aaggtgatgc tgaagcagcg ctcgggagcc atcgtcaaca
      961 tcgcgtccgt ggtcgggcgg atgggccagg ctggacaggc caactacgcg gcatcgaagg
     1021 gcggcctgat cgccctgacc aagtccgtgg cgaaggaagt ggccggccgc aatatccggg
     1081 tgaacgccgt cgccccgggc ttcatcgaga cgcagatgac gcatgttctc tcggacgagg
     1141 tgaagaaggc ctggctagag cgcatcccgc tcaaccgcgc cggaacgatg gaggacgtgg
     1201 caaaggtcgt ggccttcctg gccagcgacg atgcgtctta tctcaccgga cagacgatcg
     1261 gaatcgacgg tgggatgctc atgtcggagt agtgaagcgc gggagccttg acgggaggag
     1321 aggacgcgcc gtggccgttt cggtgcgagc cgcagcgggt catgagacgc tcctgacttg
     1381 aatggctcca gtgcagagca tccatccgga gctacttctg gatggatccc ggaggagcgc
     1441 ccgtgaggag gccaagccgc cgagcacggg ccctaaaggg aacaacgcgg gcaccactcc
     1501 cgcgtcgatg gatggaggag gtataccaaa tggatcagtc agtgtttgag cgcgtgcgta
     1561 aagtgattgt tgagcagctc aaggtgagcg aggatgaggt gacgcccgct gcgagcttcg
     1621 atgatctggg cgcggactcg ctggccaagg tcgagatggt gatggcattc gaggaggagt
     1681 tctcggtcga gattcccgac gaggatgccg agaagatcaa cacggtgggc caggccgtga
     1741 gctacatcga gtccaagtcc gccgagtaag aggcgaacat gtcgagacgt agggtagtcg
     1801 ttaccgggct gggcgcgatc acaccgctcg ggaacactgt agcggcctac tgggaaggtc
     1861 tcaaggcggg ccggtcgggc gccggcccga tcacccgctt tgatgccagc cccttcacca
     1921 cgcggattgc atgcgaggtg aaggggttca acccagagga gtacattgat ccgcgcgagg
     1981 tccgccatat cgaccgtttt gcccagttcg cgatcgcggc ggcgaagcag gcggtggcgg
     2041 attccggcct ggagatcaca ccggagaatg ccgagcgcat cggggtggtg atcggatcgg
     2101 gtattggcgg gatcgagacg tgggagaaac agcacgcgat ctacctcaac cgcggcccag
     2161 gccgaatcag cccctatttc gtgccgatga tgatctgcga catggctgcc ggtcatgttt
     2221 ccatcacgct cggttgtaag gggccgaacc tcgccaccgt caccgcctgc gcgaccggca
     2281 cgcacgccat cggcgacgct gcgtcgatca tccggcgtgg tgatgccgac gtgatgatcg
     2341 ccggtggcac cgaggctgcg atcaccccga tggggatcgg cggtttctgt gccgcgcgcg
     2401 ctctctccac gcgcaatgat gagccggagc gggccagtcg ccccttcgac aaggatcgcg
     2461 atggcttcgt acccggagag ggggttggcg tgctcgtgct ggagagcgag gagcacgctc
     2521 gggctcgtgg cgcgatgatc tacgcggagg tagccggcta tggcatgacc ggcgacgcgc
     2581 accatattac ggcgccggca cccgggggcg aaggcgctgc gcgcgcgatg ctcctggcct
     2641 tgaaggatgc cggcatggcg ccatccgatg tcgattatat caacgcgcat ggcacctcga
     2701 cgcaggccaa tgatgtcgcc gagacggcag cgatcaagcg cgcctacggc gagcatgccc
     2761 ggaagctgat ggtgtcgtcc accaagtcga tgacgggcca cctgctcggg gcagcgggcg
     2821 gtattgaagc gatcgcctgt gctctggccg tgcgctatgg ggtcgtgccc cccaccatca
     2881 actacgagac cccagacccc gaatgtgacc tggactacgt gccgaacaca gcgcgcgagg
     2941 tccgggtgcg ggccgcgatg tcgaactcgc tcgggttcgg cggacacaac gcgtgcctca
     3001 tcttccgtgc cttctgaacg ggcaggcggg gccaccatcg gccccgccgc ccgcattcgt
     3061 ctaacccccg gccccttccc taccctctgg ccctacgtcg gccccagggg aagggagcga
     3121 ctgcgtgccg ggcgcagaga gacttccgag cggccaatgc caggcgcgcc ccaccatgcc
     3181 catccgtgcc g
//
LOCUS       DAOSCV010000506         3190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19445_length_3190_cov_2.658054, whole genome shotgun sequence.
ACCESSION   DAOSCV010000506 DAOSCV010000000
VERSION     DAOSCV010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19445_length_3190_cov_2.658054"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2283
                     /locus_tag="PLY56_12320"
     CDS             <1..2283
                     /locus_tag="PLY56_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22312.1"
                     /translation="GVDAARLQEGRAIRQSLGLSAGSIQLTGRFQEIDRSFALPQRFL
                     SAAGSGETAKLLQELAQQSGTRRTALGMTAGLSKGWKLTGGFDALDDGAGRIHRQEVG
                     IASDVLQIKSLHQDVGAQFAGFNRFGDAEVQKMAAQKGVKRSALQWALAPSKQFGIQG
                     STDTHRDASGAVDARSLALHLGAFQATERRRAVSGFKTVGALEAGDQALAKLGQMALR
                     EQTAALALGGKTRLAFASDTASDTGGRFQDERYEFASPTLVARARFRSIDAGFAGAKG
                     LASVDKRFAGLEQLCGQRERAYDLDYVGSTWFGLRNHYSQTRAMDADATETWQRQVFS
                     NTMDLNLGRTSAQIHRLQSDTAQTSGETLRHRLERYRLRHNFQKESFLLAEHTQQTDH
                     DAEGERKVLQTNRLQGKLDVASLFRMEGDRVERYGTDRNAEYEDRLVLSRQFGKMGVT
                     WNFRDHQNAEGEMDVQKLSLTGTLRKGLQLTGSRETTRTQAAPDDSTTVAPAQHNSDT
                     RATLTQALGKAGKLSLSYAQLTAADAMRSREYASRLETPIGRLRLTADFANRQAAGAP
                     TVVIQGFTLADEAKERRLTWSCHYRHRSAAAGTEKETKGWIIGYNGAGARPLKLTASY
                     LRNAEAKDGKVTPGKQTRVEMNTGISRRLTLGASYINNVDENARKADERFQITLSGPI
                     GEDEKLSLLGTTQRLFEPEGTTDVKRTRVETLRLEYSHGLNESQAFSIGADLCWREYR
                     GQTAGRHCEYRSDIRWQRSF"
     gene            complement(2509..>3190)
                     /locus_tag="PLY56_12325"
     CDS             complement(2509..>3190)
                     /locus_tag="PLY56_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22313.1"
                     /translation="LFPRNQLYEVLPDTWFAEGKVKLSGFDVVILPVACHLERAFTQQ
                     LVEFLRAGGVVIASGAPGLYDQLGRPDGSLLAAAGLSARRESKPGEAWRFSYGAPVNE
                     RGWVEAKVGKGSLLLLPSSLAGLKTRDALAERVRAHAVPAAEAPGTTLELLLRALPDG
                     RYLLCALNTSPDAPTTGDVVVRGSFTRVADIDMPRAFPVTAKRAEERTRFRITLDPGG
                     TAYYLLAR"
BASE COUNT          616 a         1061 c         1063 g          450 t
ORIGIN      
        1 ggcgtggatg ccgcgcgtct gcaggaaggc cgtgccatcc ggcaatctct cggcctttcg
       61 gcggggagca tccagctcac cgggcgcttc caggagatcg accgcagctt cgcgctcccc
      121 cagcgcttcc tgagcgccgc gggctcgggc gagacagcga agctcctcca ggaactggcg
      181 caacagagcg gcactcgccg caccgcgctg gggatgaccg cgggcctctc caaaggctgg
      241 aagctcaccg gcggcttcga tgccctcgat gatggcgcgg gccgcatcca ccgccaggaa
      301 gtgggcattg cgagcgacgt gctccagatc aaatcgctgc atcaggatgt gggcgcgcag
      361 ttcgccggct tcaaccgctt cggcgatgcc gaggtgcaga agatggcggc acagaagggc
      421 gtgaagcgca gcgcgctcca gtgggcgctc gccccaagca agcagtttgg catccagggg
      481 agcactgata cccatcggga tgccagcggc gccgtggacg cgcgctccct ggcgctccat
      541 ctgggggcgt tccaggcgac ggagcgcagg cgggccgtct ccgggttcaa gaccgttggc
      601 gcacttgagg ccggtgacca ggcactggcg aagctggggc agatggcgct gagagagcaa
      661 acagccgcgc tggccctagg tggcaagacg cgcctcgcct tcgcttccga caccgcatcc
      721 gacacgggcg gccggttcca ggatgagcgc tacgagttcg cgtcgcccac gctcgtggca
      781 cgcgcccgtt tccgctccat cgatgccggg tttgccggcg ccaagggcct ggcgtccgtc
      841 gacaagaggt ttgccgggct ggaacagctg tgcggccagc gcgagcgcgc ctatgacctg
      901 gactacgtgg gctccacctg gtttggattg cgcaaccact atagccagac gcgcgccatg
      961 gacgcggatg ccactgagac gtggcagcgc caggtgttca gcaacaccat ggatctgaac
     1021 ctggggcgga cctcggccca gatccaccgc ctgcaatccg acaccgcgca gacatcggga
     1081 gagaccctcc gccaccgcct ggagcgctac cgcctgcgcc ataacttcca gaaggagagc
     1141 ttcctcctgg ccgagcatac gcagcagacg gaccacgatg ccgagggaga gcgcaaagtg
     1201 ctgcaaacga accggctgca ggggaagctg gatgtggcat ccctcttccg catggaaggc
     1261 gaccgcgtgg agcggtacgg aacggataga aacgccgagt atgaggaccg cctggtgcta
     1321 tcgcggcagt ttgggaagat gggagtcacc tggaacttcc gagaccacca gaacgcggaa
     1381 ggcgaaatgg atgtgcagaa gctctcgctc accggcactc tccgcaaagg cctgcagctc
     1441 accggcagcc gggagacaac gcgaactcag gccgcgcccg acgatagcac caccgtcgcc
     1501 ccggcccagc ataacagcga cacccgggcc actctgacgc aggctctggg caaagccggc
     1561 aagctcagcc tctcctacgc ccaactgacg gcggccgatg cgatgcgctc gcgcgaatat
     1621 gcgagccgcc tggagacgcc gatcggccgg ctccgcctga cggccgattt tgccaaccgc
     1681 caggccgctg gcgcgcctac cgtggtcatt cagggcttca ccctggcgga cgaggcgaag
     1741 gagcgccgcc tcacctggtc ctgtcactac cgccaccgct ccgccgccgc tggaaccgag
     1801 aaggagacga aggggtggat cattggctat aatggagccg gcgcccgccc tctcaagctg
     1861 acggcatcct acctgcgcaa tgccgaggca aaagatggca aggtgacccc aggcaagcaa
     1921 acacgcgtgg agatgaacac cggtatcagc cgccggctga ctctgggagc gagttacatc
     1981 aacaacgttg atgagaacgc gcgcaaggcg gacgagcgct tccagatcac cctcagcgga
     2041 ccaatcggcg aggatgagaa gctctcgctc ctgggcacca cccagcgcct ctttgaaccc
     2101 gaaggcacca cagacgtcaa gcgcacccgc gtggagaccc tgcgcctgga gtatagccac
     2161 ggtctcaacg agtcgcaggc gttcagcatc ggcgcggacc tgtgctggcg tgagtatcgc
     2221 ggccagacgg ccggccgcca ttgcgaatac cgctccgata tccgctggca gcgcagcttc
     2281 taggcgggcc ggcagcacgc gctgaggaga gttccctcag gacgggcagg tgccgcgcat
     2341 ggcatgccgt agattccctg gtgtatataa ggtagattct ccctaaaaaa gagaaacgtg
     2401 cgcgagaaaa acgtgtgggg cgcgagcacc cagctcacgc ccctgaaaac gcgaggcggg
     2461 cggcaagcgc ctggatgcac accaggctat cttgccgccc gcttgcatct agcgtgccag
     2521 caggtagtag gccgtgcctc cgggatcgag ggtgattcgg aagcgcgtgc gttcctccgc
     2581 gcgcttcgcc gtgaccggga aggcccgagg catatcgata tcggcaacgc gcgtaaagga
     2641 gccacgcaca acgacgtcgc ccgtggtcgg ggcgtcgggg ctggtgttca gcgcacacag
     2701 gaggtaacgc ccatccggga gcgcgcgcag gagcagctcc agggtcgtgc cgggcgcctc
     2761 ggcggcgggc acggcgtgcg cgcgtacgcg ctccgccagg gcatcgcgcg tcttcaggcc
     2821 ggccagcgag ctcgggagaa gcagcagtga gcccttcccc acctttgcct ccacccagcc
     2881 gcgctcgttg accggcgctc cgtagctgaa ccgccaggcc tcacccggct tgctctcgcg
     2941 ccgggccgaa agccccgcgg cggcgagcag tgagccatcg ggccggccaa gttgatcata
     3001 caatccgggc gctcccgagg cgataacgac gccgccagcc cggagaaact cgacgagctg
     3061 ctgggtgaaa gcacgctcga ggtggcaggc caccggcagg atcacgacgt cgaagccaga
     3121 gagcttcacc ttgccctcgg cgaaccaggt atccgggaga acctcgtaga gctggttgcg
     3181 cgggaagaga
//
LOCUS       DAOSCV010000507         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19494_length_3183_cov_3.133632, whole genome shotgun sequence.
ACCESSION   DAOSCV010000507 DAOSCV010000000
VERSION     DAOSCV010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19494_length_3183_cov_3.133632"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLY56_12330"
     CDS             <1..522
                     /locus_tag="PLY56_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOJ22314.1"
                     /translation="VRAATHAGMERLVKLGARDVIHPELEGGVEVLRHTLVTLGYPAL
                     EAQQYADGVRRAQYHSAFSAGIGRQVCDRLMRIVPGMDVAWVTLDDACPVLGMTLADA
                     GIREQTGASVVAVWRGQQVIANPEADTVLRAGDMLGVLGEKAHIEAACRLLAGEKALE
                     AHPSDPPMASAPV"
     gene            914..2119
                     /locus_tag="PLY56_12335"
     CDS             914..2119
                     /locus_tag="PLY56_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22315.1"
                     /translation="MSEKWKRNTTLFLSSQAISLFGTSLVQYAITWYITLETQSGVMM
                     TLAVICGFLPTVLLSPFAGVWADRYDRKMLIVLSDSMIAVATLILAILFWMGYEAVWL
                     LFVVSGIRALGSGVQTPAIGAFLPQFVPQEKLTQVNATNTSIQSAVMLVSPMLSGALL
                     AMTGIEAIFFIDVITALIGVSILLGLVKVPSHTRAQGEPMGTYFADMRAGLDYVMSHG
                     FVKRICLFNAVFFVLIAPTAFLTPLQVARSFGEDVWRLTALEIAYSGGMIVGGCVMAA
                     WAGFRNKVYTMALSNLIVGASAVALGVVPLFWVYLFFVGLMGLAFPLFNTPFTVLLQQ
                     KIEGDYLGRVFGVIGVVTSMAPLAMLLFGPVADLMRIEWLLIGTGVLMCVVSFLMLAS
                     RVLVAAGEP"
     gene            2258..2332
                     /locus_tag="PLY56_12340"
     tRNA            2258..2332
                     /locus_tag="PLY56_12340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2290..2292,aa:Gly,seq:ccc)
     gene            2374..2449
                     /locus_tag="PLY56_12345"
     tRNA            2374..2449
                     /locus_tag="PLY56_12345"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Cys,seq:gca)
     gene            complement(2840..3025)
                     /locus_tag="PLY56_12350"
     CDS             complement(2840..3025)
                     /locus_tag="PLY56_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22316.1"
                     /translation="MRSSFTDGAWVVFWEVEHLQELHMEDRIRIAHIQGFGRRGPYKR
                     NFAPEGPMLIEHPGATR"
BASE COUNT          519 a          950 c         1060 g          654 t
ORIGIN      
        1 gtccgtgcgg ccacgcatgc ggggatggag cgcctggtga agctgggcgc gcgcgacgtc
       61 atccacccag agcttgaggg tggggtcgaa gtgctccggc ataccctggt cacgctcggc
      121 tacccggcgt tggaggcgca gcaatacgcg gatggggtga ggcgtgccca gtaccactcc
      181 gccttctctg ccggtatcgg gcgtcaggtg tgcgaccggt tgatgcgcat cgtgccaggg
      241 atggatgtcg cgtgggtgac gctggacgat gcctgccccg tcttggggat gacgctggcc
      301 gatgcgggca tccgcgagca gaccggcgcc tcggtggttg cggtctggcg cgggcagcag
      361 gtgatcgcca atccagaggc tgacacggtg ctccgcgccg gggatatgct tggcgtgctt
      421 ggagagaagg cgcatatcga ggccgcgtgc cggctgctgg cgggtgagaa ggcgctagag
      481 gcgcatccat ccgatccgcc catggcgagc gccccggtgt agggtatccg ctttgcccca
      541 aggggccgct cgctcccatc aggacctcct ccctggagag atgcgcgccg tcgcggcgct
      601 gtggccccct cctgccagcg tggcatgctg gtactcgtgg cgtagcctga acgccctgcc
      661 cgccagcacc cgccctcctg ccatagcatc ctgaccgccg ctctccaggt gttggtaggc
      721 cctcattcag cgccgcgggt tgccgatggc tctcatccaa cgcctcggca ccaggcatcc
      781 tttccgtttc ctgcaaggag gaggcgacgc cctcgcactg cgttgttctc agcggtttgg
      841 ccggcggaat ccgcgcgagg gcgcgggctg cgttctattc gctgtcgcgc actccgcgct
      901 gatggataac cctatgtcgg agaagtggaa gagaaacaca acgctctttc tgagcagcca
      961 ggcgatctcc ctgtttggga cgagtctggt tcaatatgcg atcacgtggt atatcacgct
     1021 ggagacgcag tcgggcgtga tgatgaccct tgcggtcatt tgcggttttc tgccgacggt
     1081 ccttctctct ccctttgcgg gggtatgggc cgaccgctac gaccggaaaa tgctgatcgt
     1141 cctgtcagat tccatgatcg ccgtggcgac gctcatcctg gccatcctgt tttggatggg
     1201 gtacgaggcg gtctggctgc tcttcgtggt ctcgggcata cgcgcgttgg gctcaggagt
     1261 tcagacgccc gcgatcgggg cattcctgcc gcagttcgtg ccacaggaga agctcaccca
     1321 ggtgaacgcc accaacacga gcatccaatc cgcggtgatg ctagtctctc cgatgctgag
     1381 tggcgcgctg ctggcgatga ctggaatcga ggcaatcttc ttcatcgatg tgatcacggc
     1441 cctcatcggt gtttcgatcc tgctggggct ggtgaaggtg ccatcccata ccagggctca
     1501 gggcgagccg atgggcacct actttgctga tatgcgcgcg ggcctcgact atgtgatgag
     1561 ccacggcttt gtgaagcgga tctgcctctt caacgcggtc ttcttcgtcc tgatcgcgcc
     1621 caccgcgttt ctgacgccgt tgcaggtggc gcggagtttc ggcgaggacg tgtggcgcct
     1681 gaccgcgctg gagatcgcct actcgggcgg catgatcgtg gggggatgcg ttatggctgc
     1741 ctgggccggc ttccggaata aggtgtacac gatggccctc tcgaacctga tcgtcggcgc
     1801 gagtgccgta gcgctcgggg tggtccccct gttctgggtc tacctcttct ttgtggggtt
     1861 gatggggctg gcctttccgc tcttcaacac gcccttcacg gtgctgctgc aacagaagat
     1921 cgagggggat tacctgggcc gggtcttcgg cgtgattgga gtggtgacca gcatggcgcc
     1981 gctcgcgatg cttctgtttg ggccggtcgc cgacctcatg cgcatcgaat ggctgctcat
     2041 cggcacgggc gtgctgatgt gcgtggtgag ttttctcatg ctggcgagtc gggtgctggt
     2101 ggcggcagga gagccctagc ggctccgggc gggcgcgacg agggcggggg aacactcgtt
     2161 gccgcgccct gctgcaatat cccgggacat ctggaaaaca gagtgcagat tccctattgc
     2221 acctagtggc agggtatgct ataataatgg cggttgcgcg ggcgtagctc agtggtagag
     2281 cgtctgcttc ccaagcagaa tgccgtgggt tcgagtccca tcgcccgctc caaagggata
     2341 cgccattctg tgacggagcg gtgtatccac tctggcgtgg tacccaagtg gctaagggag
     2401 cggtctgcaa aaccgttatt caccggttcg attccggtcc acgcctccaa tatgtagggc
     2461 tctaagcgag gccagaccac aacatttcgt ggtctggcct tcttgattct ccggaccgcc
     2521 ttcccctggg tgcaacaagg gtgcaacaca gatagacccc cacgcatgct atagacgcca
     2581 tgcggcacgc gagaggagca gcgcatcact cggtgtgccg ccatggatgg gagaacagcg
     2641 gtcctggagg cgtgtggggt gaccggatgc cccgccgaag tcggcggcga gcgggacggc
     2701 tgccagggca atgtcgggca atcggacgcc tcggccatgc ggtgaggtat gtgtctcttt
     2761 cgcgggctca ccgaattctc ccgcgccggc gatggagggc tcggtggtcg tctatgaagc
     2821 gcgggcgcat ggtcaccctc tatcgggtgg cccccggatg ctcgatcagc atcggacctt
     2881 cgggagcgaa gttccgcttg tagggccctc gtctgccgaa gccctggatg tgcgcgatac
     2941 ggatccggtc ctccatgtga agctcctgga ggtgctccac ctcccagaag accacccacg
     3001 ccccatcagt gaaagaggat ctcacgccgg gcctctatct cggttacgca ggagagttac
     3061 agcagataca cctcccaggt ggagaaaacc gttccgaatg agcgcacgcg gggcgatcaa
     3121 agggttgaca caaacgcgga atgcagacta taattctcat gacaggcgct cgatacatat
     3181 ccg
//
LOCUS       DAOSCV010000508         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19536_length_3179_cov_3.139885, whole genome shotgun sequence.
ACCESSION   DAOSCV010000508 DAOSCV010000000
VERSION     DAOSCV010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19536_length_3179_cov_3.139885"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLY56_12355"
     CDS             <1..281
                     /locus_tag="PLY56_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ22317.1"
                     /translation="DVERPLAGRHVLVVEDIVDTGTTLAFLLGTLASRQAASVRVVSL
                     LDKPGRRVNQVQVDFAGFTVENLFVVGYGMDHAEAYRHLPDICALEEV"
     gene            complement(330..1220)
                     /locus_tag="PLY56_12360"
     CDS             complement(330..1220)
                     /locus_tag="PLY56_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ22318.1"
                     /translation="MPAPRHHTTSESRNRFFTGAMLESLLRPEAWRLVAAALPPMVEP
                     VEDERHQSWMRAHTHSHAHQEILIPLAGEGRFGLAGRIYPSAPGTIFVIDSFEEHDVQ
                     YPEWMPDLDHLWISALRDHCVVRLITVREGTTRVSGAWSCLISREEAGPPLDPVPVRA
                     EMPPEVARLRMVGALSLLVGALIRRGYGAEQAEERDSFQARIVQSVREHIEHTAGNGA
                     TLEHLARIAGYSTFHFLRLFKKHTGCTVHEYIDRCRLRRAREMQAQGRTRKEIAVALG
                     FSCPAAFSRWYRTRSTRPPS"
     gene            1567..2741
                     /locus_tag="PLY56_12365"
                     /pseudo
     CDS             1567..2741
                     /locus_tag="PLY56_12365"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
     gene            2738..>3179
                     /locus_tag="PLY56_12370"
     CDS             2738..>3179
                     /locus_tag="PLY56_12370"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ22319.1"
                     /translation="MRIGFGKVDITPRVGVELCGFGPFLNRHSIGVRDRLWARAMAIE
                     AGGRTAVVVSCDLVAVVLPTTKQVRELVKEAVGLGEDAVMVHCTHTHSGPATHNSLGW
                     GVPDLPYMELLPRRIAEACIQAVRNLAPAELCHAEVPCEGIGLNR"
BASE COUNT          511 a         1045 c         1109 g          514 t
ORIGIN      
        1 acgacgtcga gcgccccctg gccggccggc acgtcctcgt cgtggaggat attgtcgata
       61 ccggcaccac cctggcgttc ctcctcggca cgcttgcgag ccgccaggcc gcctcagtgc
      121 gcgtggtgag cctcctcgac aagccgggcc ggcgcgtgaa ccaggtgcag gtcgatttcg
      181 ccggcttcac cgtggagaac ctcttcgtgg tgggctacgg catggaccac gccgaggcct
      241 accgccacct ccccgatatc tgcgcgctcg aggaggtctg aggcccgctg ggcgcgcttg
      301 gaacttgccg gaccggtgcc cgctgcctgt caggatggcg gccgggtgct cctggtgcgg
      361 taccagcgcg agaatgcggc ggggcaggag aagccgaggg ccacggcgat ctcctttcgc
      421 gtgcgcccct gcgcctgcat ctcgcgggcg cggcgcaggc ggcagcggtc gatgtactcg
      481 tggacagtgc acccggtgtg cttcttgaag aggcgcagga agtggaaggt gctgtagcca
      541 gcgatgcgcg ccaggtgttc cagcgtggcg ccgttgcccg ccgtgtgctc gatgtgctcg
      601 cggacgctct gcacgatgcg cgcctggaag gagtcgcgct cctccgcctg ttcagcgcca
      661 tagccccggc ggatgagggc accgaccagg agagagagtg cccccaccat gcgcaggcgc
      721 gccacctccg gcggcatctc cgcgcgcacc ggcaccggat ccagtggcgg cccggcctct
      781 tcccgcgata tcaagcagct ccacgcccct gagacgcgcg tagtaccctc cctcaccgtg
      841 atcagccgga cgacgcagtg gtcgcgcagc gcgctgatcc agaggtggtc gagatccggc
      901 atccactccg gatactggac gtcgtgctcc tcaaagctgt cgataacgaa gatcgtgccg
      961 ggcgcgctgg ggtagattcg tcctgccagc ccgaagcggc cctcgcctgc caatgggatc
     1021 aggatctcct gatgggcgtg ggaatgcgtg tgtgcgcgca tccagctttg gtgacgctcg
     1081 tcctccaccg gctccaccat cggtggcagg gcggcggcga ccagcctcca cgcctcgggg
     1141 cgcagcagag actccagcat cgcgccggtg aagaagcggt tgcgcgattc agaagtggtg
     1201 tggtggcgtg gtgctggcat cagcggaact actcctcgcc aggcgagcgc ccatccagaa
     1261 gcgggctccc cgtggccggc tggcgtcctc tctctgctcc ggaccggagt tggcagccaa
     1321 gtatagcaag aattgtatcg caatggcaag gatggtgtct tggcgtttgc ctcggaacca
     1381 tctataatgc gggcatggag agaagccggc gtctagcgca agagtggtat ctcgtgaaga
     1441 cgaccggcgg ggaggttctc gatggcaaag agtgatgtct cgaagctcgc gctcctgggc
     1501 gggcgtccgg cggtcgaccg gccggcgccg gcgtggccgg tgacgggcga cctggaagtt
     1561 gcgtggatgg agggcgtggt ccgcagcggc aagtggagct ggctgggccc gcacgagcgc
     1621 gctttctgcg aggagttcgc cagcttcatc ggcgcccggc atgccatgct cctggccaac
     1681 ggcacggtga cgatccaggt tgccctccag gcggtcggcg tccggccgga gatgaggtga
     1741 tcgttccggg gctcacctgg acggcgacga tgcaggccgc gctcgacatt ggcgccaacg
     1801 ttgtcttggt ggatatcgac ccggaaacct actgcatcga cccggcggct gtcgaatcgg
     1861 cgatcacgcc gaggacgaag gcagtggtgc cggtccatct ctacggctgc atgtgcgata
     1921 tggacgcgct aaccgacatc gcgaagcggc acaacctgaa gatcgtcgag gatgtggcgc
     1981 accagcacgg cagccgctgg cggggtcggg gcgcgggtgc ccttggagac gctgcgagct
     2041 tctccttcca gcagagcaag gtcctcacct gcggggaggg cggcgcggtc acctgcaacg
     2101 atgatgtggt gttcgagacc gccttttgcc tgaagcacgt cggctggatg ccggacctgc
     2161 agacccgcgg caatcgctac gggcacaact accggggcac cgagatgcag gccgtgctcc
     2221 tgcgcggtgg gctgcgccgc atcggtgagc agctccgcat ccgcgaggag aacgcgcacc
     2281 tcctcgccga gggcttccag gagatcggtg gggcgatccg ggtggcccgg cgcgatccgc
     2341 gcgtcacgcg ccaatcctac tacgcgctca cgctccactt cgacaaggcg aaggccggcg
     2401 gcctggatcg ggcccaatac gtggcggcgc tcgcggccga gggtctcccc ctctcgccca
     2461 cctacgcacc ggtctaccgg aaccctctcc tcaacctgta tcacgaggcg agcccggtgc
     2521 ccttccgcga gacggttccc caggattacg ccaacctccg cctcccggtc accgagcgcg
     2581 tcgcggaaga ggatggcctg gtcttgatgc acatgcacct gctgggagag cgggcctaca
     2641 tcgagcagct tctcgccgcc gcgcgcaagg tgcaggacca cctgccggag ctggcgaagc
     2701 acttcgcgca ggaggcatcg ggggaacagg aggcatcatg agaatcggct ttggtaaggt
     2761 agatatcacc ccgcgcgtgg gcgtggaact gtgcgggttc gggccgttcc tcaaccggca
     2821 ttccattggc gtgcgcgacc gcctctgggc cagggcgatg gcaatcgagg cgggcgggcg
     2881 caccgccgtc gtcgtgagct gcgacctggt agcggtggtc ctgcctacca caaaacaagt
     2941 gcgggaactg gtgaaggaag ccgtgggcct cggcgaggac gcggtgatgg tccactgcac
     3001 gcacacgcac agtggcccgg ccacgcacaa cagccttggc tggggcgtgc ccgacctccc
     3061 ctacatggaa ctcctcccgc gccgcattgc cgaggcgtgc atccaggcgg tgcgcaacct
     3121 ggctccagcg gagctatgcc acgccgaagt gccctgcgag gggatcgggc tcaaccgcg
//
LOCUS       DAOSCV010000509         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19578_length_3175_cov_3.329167, whole genome shotgun sequence.
ACCESSION   DAOSCV010000509 DAOSCV010000000
VERSION     DAOSCV010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19578_length_3175_cov_3.329167"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PLY56_12375"
     CDS             complement(<1..432)
                     /locus_tag="PLY56_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOJ22320.1"
                     /translation="MGFLATLKQFVSSLHETPDGPMLAVARLLVVYILLRYFLNNIVG
                     RVIVPLVAKEFARDRKERAARVKTLAGVIRSTGNYLLTFIIGLMALNAFGVDTTPVLA
                     SAGVIGLAVGFGSQKLVRDVTTGFLLLAENQLDVGDYVTISG"
     gene            complement(411..1148)
                     /locus_tag="PLY56_12380"
     CDS             complement(411..1148)
                     /locus_tag="PLY56_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ22321.1"
                     /translation="MLSPTRICTYLECPRKYRYIYIDKLPRYFGARAYFSFGSSLHRA
                     LQEYHEAGGAQTQNAEALISRLEQNWIGAGYESAEDERARLLQGREILAAYHESAATQ
                     EGVTLYTEKLLRIDMGDYALTGRIDRLDSLPDGGLEVIDYKSGERLPTSEEVADDLPL
                     AIYQLLCVRTFECPRVKASIYHLRTGSKVSTVRTAQEVEPVAEGIHALYARMRAEERY
                     DPIVGDHCSHCDFLDRCRRQGWDSSRL"
     gene            complement(1379..1711)
                     /gene="trxA"
                     /locus_tag="PLY56_12385"
     CDS             complement(1379..1711)
                     /gene="trxA"
                     /locus_tag="PLY56_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ22322.1"
                     /translation="MAQNVVELTDQTFESEVIKADLPVVVDFWAPWCGPCRAMAPVLE
                     QYAQEQAGVIKVAKINVDDHGSTAAQFGVMSIPTFLIFSGGKPVGQFVGAMPKEEFAK
                     RVAAALGK"
     gene            2258..>3175
                     /locus_tag="PLY56_12390"
     CDS             2258..>3175
                     /locus_tag="PLY56_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22323.1"
                     /translation="MSARVRALTEALRAHRGQAVSPGDCVLSPRARRGLLALLAAVLA
                     SQLGCASHVSRAPGSAGPELNAPTRAVAEGWQYRGMTFISWTRDGYFQPSTLVSLDQM
                     VAHNVRWVAIIPHWFMDNKESTRIYPDPARYTPSDESVRYIIQQAHARGLKVMLKPHV
                     DPKDGVWRGSITPTSPSAWFESYRAFILHYARIAAETGVELFSMSNELRSMTGPPYAS
                     NWRSLVADVRGVYPGKLTAAANWGPRNWAEYLRIEWWDAAQGVARTAMLPPLQLQVAP
                     GAAAATTSVDAAAPAGRAGADAPSGDAAHA"
BASE COUNT          581 a         1047 c          991 g          556 t
ORIGIN      
        1 gccactgatg gtgacatagt cgcccacgtc caactggttc tctgccagga gcaggaagcc
       61 ggtggtcaca tcgcgcacca gtttttgaga tccgaagccg acggcgaggc caatcacgcc
      121 ggcgctggcc agcaccgggg tcgtgtccac cccaaacgcg ttgagcgcca tcagaccgat
      181 gatgaaggtg agcagatagt tgcccgtgct gcggatcacg cccgccaacg ttttgacccg
      241 ggctgcccgc tccttccggt ctcgcgcgaa ctcctttgct actaatggga cgatcacgcg
      301 gcccacaatg ttgtttaaga agtagcggag caagatgtag acgacaagca gcctcgccac
      361 ggcgagcatg gggccatcgg gcgtctcgtg caaagaggag acgaactgtt tcagagtcgc
      421 gaggaatccc atccttgcct ccggcagcgg tcgagaaagt cgcaatggct gcaatgatcg
      481 cccacgatgg ggtcataccg ctcctcggcg cgcatccgcg cgtagagcgc gtggatccct
      541 tcggcgactg gctccacctc ctgggcggtg cgtacggtgg aaaccttgct tccggtacgc
      601 aggtggtaaa tgctcgcctt tacgcgcggg cactcgaagg tgcgcacgca cagaagctgg
      661 tagatcgcta ggggcaggtc atcggccacc tcctccgacg tgggcaagcg ctcgccgctc
      721 ttataatcga tgacctcaag gccgccatcg ggaagcgagt cgaggcggtc gatacgaccg
      781 gtgagcgcgt aatcgcccat gtcgatgcgg agcagcttct cggtgtagag cgtgacgccc
      841 tcctgagtgg ccgcgctctc gtggtaggcg gccaaaatct cccggccttg caagaggcga
      901 gcgcgctcat cctccgcgct ttcgtagccg gcgccaatcc agttttgctc caggcgggag
      961 atgagcgcct cggcgttctg tgtctgggcg ccacccgctt cgtggtactc ctgcaacgcc
     1021 cgatgaagcg agctaccgaa gctgaagtag gcgcgcgcgc cgaaatagcg cggaagctta
     1081 tcgatgtaga tgtaacggta tttccgcggg cactcgagat aggtgcagat ccgcgtgggg
     1141 ctaagcaccc attttcgctt ccgctgggat gcattcgccg gcctttcggt gcgagaccgg
     1201 cttcggggct gcttcccgcc cggatcggtg acttcgattg ccctggagag atcgtccgcc
     1261 atggcgccag tatagcgcac actccgtgag aagtcaaacc gaggcaggag acacggcggg
     1321 atccgagagg gtggcgccac cggatcccgc tccggaaaga gcacgtaggg ccaacgacct
     1381 acttgccaag agccgctgcc acccgcttgg cgaactcctc cttgggcatt gcgccaacga
     1441 actgcccgac cggcttgccc ccggagaaga tcaggaaggt gggaatgctc atgacgccaa
     1501 actgagccgc cgtgcttccg tgatcatcca cattgatctt ggcgaccttg atgactcccg
     1561 cctgctcctg ggcatactgc tccaatacgg gagccatcgc ccgacacggg ccgcaccagg
     1621 gcgcccagaa atccaccacc accgggagat ccgccttaat cacttccgac tcgaacgtct
     1681 gatcggtcag ctcgacaaca ttctgcgcca tttagtcctc catcctctcg cgtccggcta
     1741 tacccattct tttccaacac gggcctgcct gtcaaacgaa gtgcgcagag cgcccatgat
     1801 ccactcgcgg tggctggcgg tagaagccct aagcgagttg taccccgcct gcattcaacc
     1861 aacacgggcc cgctacccaa atcgacgcgg gcgcgatcgt accgctccag cgtcgcagtg
     1921 ccagtcggga gccccatcac cgctcatggg cgatcctatc gagacagttc cccctgaagg
     1981 gcgcccgttc ctgtctcgag cgctctcgga gaatccgggc gcagccggag accgcgcgag
     2041 acccacgttg tacgcgcagg atgggggatg agagcaccca tccttaacaa cgcgcgggtg
     2101 cgccccaagc cggatggcac tcattctgca gccccatgcc ggttgacgcg ggcgtgcttg
     2161 cgacccaggc gtgggcgatc caacacaaca tgtccactcg aaggaggggt gtgccatcaa
     2221 acgcctttcc gaggtatgca tacaccaaga gcgcgccgtg tctgcgcgcg tgcgcgccct
     2281 aaccgaggcg ctgcgcgccc atcgaggcca ggcggtatcc ccgggggact gcgtgctttc
     2341 gcccagagcg cgtcgtggcc tcctcgcatt gttggcggcg gtgctcgcca gccaactcgg
     2401 gtgtgcctcg catgtcagcc gtgccccggg ctctgcgggg ccggagctca acgcgcccac
     2461 ccgcgcggtg gcagaaggct ggcaataccg cggcatgacc tttatctcat ggacgcgcga
     2521 cggctacttc cagccctcga cgctcgtctc gctggatcag atggttgccc acaacgtgcg
     2581 atgggtagcc atcatcccac actggttcat ggataacaag gaatctaccc gtatctatcc
     2641 cgacccggcg cgctacacgc cgagcgacga atcggtgcgg tatatcatcc agcaggcgca
     2701 cgcgcgcggg ctgaaggtga tgctcaaacc acacgtggat ccgaaggatg gcgtctggcg
     2761 gggtagcatc acgccgacga gtccatccgc gtggtttgag agctatcgcg ccttcattct
     2821 gcactatgcc cgcatcgccg cggagacagg cgtggagctc ttctcaatga gcaacgagct
     2881 ccgctcgatg accggtccgc cctatgcgtc caactggcgg tcgctggtcg cagatgtgcg
     2941 aggcgtctat cccgggaagc tgaccgccgc ggcaaactgg gggccccgca actgggcgga
     3001 gtacctgcgc atcgaatggt gggacgcggc gcagggcgtg gcgcgtaccg cgatgctgcc
     3061 gccgttgcag ttgcaggtcg cgccgggcgc cgccgccgcc acgacctccg tcgatgccgc
     3121 ggcacccgcc gggcgtgcag gcgcggatgc gccatcgggc gatgccgcgc atgcg
//
LOCUS       DAOSCV010000510         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19593_length_3173_cov_2.792495, whole genome shotgun sequence.
ACCESSION   DAOSCV010000510 DAOSCV010000000
VERSION     DAOSCV010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19593_length_3173_cov_2.792495"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..304)
                     /locus_tag="PLY56_12395"
     CDS             complement(95..304)
                     /locus_tag="PLY56_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005550463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ22324.1"
                     /translation="MRTSTFHVEGMTCEGCASAIRRAITAPGGVERVDVDLDAKRVTV
                     VYDPGKVSEEEIRERIEDAGYDVVG"
     gene            complement(3037..3173)
                     /locus_tag="PLY56_12400"
                     /pseudo
     CDS             complement(3037..3173)
                     /locus_tag="PLY56_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase"
BASE COUNT          530 a         1058 c         1034 g          551 t
ORIGIN      
        1 aggagcgcca gcccgcgcgc gagcagcaag ggcggaagcg cccttgccac ccgctccatc
       61 cgcgagccgt tgccgaatct cgatgggagc ccgcctagcc gacgacatcg tagccggcgt
      121 cttcaatccg ctcgcggatc tcctcctcgc tcactttccc cgggtcatac accacggtga
      181 cacgcttggc atccagatcc acatcgacgc gttcgacgcc accgggcgcg gtgattgcgc
      241 gccgtatggc gctcgcgcat ccctcgcagg tcatcccctc cacatggaag gtgctggtac
      301 gcatctctcc tccttccgcc gctttcgcgc ccccgctccc gcctcaagcg gttgcaacgg
      361 catcgccttc gcctccttgg gaggagaagc gggagccgca agggaacttg cagatcaaac
      421 cggtatccgc cggcgcaccg ttccgtgccg gctctcctcc cgagagaaga ggtgtagcca
      481 tgaacagacg ctttctgccg ggcatcgccc tggtgttcct cctttcggca gtcgtgcccg
      541 ggtttcccgc gagcttcact ggcggggggg tcacgctcca gttggacgac cgagcccggg
      601 tagtgggtat ggccgttgga gagaaggcgc tgaaggtcgc tccggaaccg ctggtggccc
      661 tgtgcgatgt ttcgaccggc gagttcgtcc ccggcacggt ggtagggggc gatgccgcga
      721 cggggtggca gctcgatttc ggagcggcgc gtgcccgggc cacgctgatg gtcgctccca
      781 agggcgagcg gctgcagttt acgtgcgacc tccagggcga gaacctgccg gcgcggggga
      841 tgcttctccg ctttgccttt cccttcgatg ccatcggttg gcagtggtac caggatatgc
      901 agaccgcgaa gccgatcgcc gcggccgagg tgtgcgagaa tgtggtgccc ctgcgcgctt
      961 atgccgacct gcccgaatgg cgcgaccagc ccgcgctacg catgggctat accaaccgca
     1021 acttctgcac ggtcgttgcc gggccagtcg ggctctgcgt ggcggtgccc ctagaccggc
     1081 ctgccacctt ccgcaccgcc tataacgggc cccagaagcg cctggagatg gtttatgact
     1141 tcgcgctctc gccggacacg cgccgcccga acgcggtgag ctttgcgttt gacctttacg
     1201 catgcgaccc ggcctggggg atgcggagcg cgttgcagcg gtattaccgc atctatccgg
     1261 agttcttcaa ggtatacccg cgtgtgcaag gccagtggat ggctttcgcc cggctctccg
     1321 agatcgataa cgccaatgaa ttcctctttg gcctccaaga gggtgctccc gagccggagt
     1381 acgacgacaa gctcggcgtc ctctccgcga cctacttcac gcacgccggc atgttcgcca
     1441 acatcccggg ctacaacccg gagaaggacc cgctgcctcc ctacgagacg ctggtcgctg
     1501 ccatggaagc gcagttcgag cggaccacca agctcaaggg cctctacaaa caggtgggtc
     1561 tgcacaccgc cgaggggaag ctggacgtgc gcaaggcagc ggtgtacggg catttgctcg
     1621 cgcagtttac cctcgatccg gagctcacct atggcgagtg gactctgaag cgagctctgg
     1681 aacagacaga gcagatcaag cgaacgaaag gcgccgatct ggacggattc tactacgatg
     1741 gcctctccac gggcctggat tacaacccgg agcatttcaa gacggcggat gcaccctgcc
     1801 tgtgggatcc ggaggccaag aagccattcc tcaacaactt cttcgcctct gtcgagttcg
     1861 cgcgcgccgc ggcggagctg ctccgcccca agggccagat cacgatgatg aatggtgcct
     1921 tggagagcag cttctacgtg gcgccctggc tcgatctgtt cggcgccgag acgggcgtgc
     1981 gcattccgcg cgagagattc aactacatcc gcacgatcat ctaccacaag ccgttcctca
     2041 cgctgctcaa ggggaactac gagaaggtcc tggggcgccc tgaggtcgag ctctacatga
     2101 agcgctgcct ggcctatggc gtctatcccg gtttcttcga ctggtcgccc agcggcctcg
     2161 gccccggcgg gcagtatcac tcgcacccgc gctactacga gcgtgaccgg gatctgcatc
     2221 gcaagtacct gcccctgtgc cgggcgctcg cgctggcggg atgggagccg gtgacgtacg
     2281 cgcgctcctc cgcgcctgct gtcttcgtgg agcgcttcgg accgacgcaa gagggcattg
     2341 tctggtggac gctcctcaac gaggactcac gaccccacga gaccacgctg acgatggatc
     2401 tgggtgcgct ccgcctggat gcgcagagcg tgcgcgcggt ggatctcgtc tcaggcacgg
     2461 cgatcccgct ggcgatccag ggccagacgg cagttgcccg gctgagcgtg ccggcggatg
     2521 gcgtcctggc cattcaggtg gcgaagcccg cggcagccgc gggctggcgc gtggcgcaat
     2581 cgatcgaaac cctggaccgc ggaatccaga tgcgcgctat cgacgcggag aagcctgcca
     2641 tcccggtgca ttggcgctac agcggccaga tcacccgcga gagtgagggc acggaccatt
     2701 gcgtcgtcct tgtcgcggat ggcaagcgcg acacgactat ccggcaatgg gcgatgctct
     2761 tccaaccgga gccgaggggg gtgaaactgc gggttcgcgc cgcggctacg ggcgtgcagg
     2821 atggcgatga tgcccgcatc cgctgccagc tggcctgggt gacgcggagc ttcacgcact
     2881 acgagaacgt caccttcgac ttgccgggcg gcacctacga ctggcgcgac ttcgagttcc
     2941 cgatccggtg cgttgagcga agattgacca gcgcgcaagc ctggcccctc aaggacggca
     3001 gaacaccggc ggccgccgat acgaagcccg ccgccgaagt cggccggcgc gcagcaggtt
     3061 cagcgcttgc cagatgtaat cgggcgggcc ttcctcgccc gcgtgtgcca ccacgtgcag
     3121 gcccagttcg cggcagcggg cgaacacgcg ggcgaacttt tcgggcgggt ggc
//
LOCUS       DAOSCV010000511         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19600_length_3172_cov_3.030478, whole genome shotgun sequence.
ACCESSION   DAOSCV010000511 DAOSCV010000000
VERSION     DAOSCV010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19600_length_3172_cov_3.030478"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PLY56_12405"
     CDS             complement(<1..1721)
                     /locus_tag="PLY56_12405"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ22325.1"
                     /translation="MLWLLAFLLILAPASAETLQLDEWTGLAPDPAVTYRGKPALAWN
                     DIARVTRVEAKGAPADWTRFKTLRFATHVAKPTDSVITLVIDSGNPETEGADYYSFTF
                     TTDFTGWREFQVSLDAMASARQPVGWHQIRWVAFSSNWAHRLDPETVLHLANFTLDTE
                     PIPGNDRPAGELLANRSFELDTNYDGRPEGWGVSTWDTGSQVALDTQVARTGRCSMRI
                     DGDPQGRAGLSVGFGPEETDPKAVYLLSAWVKVEGESTHQLRTSARITSVGPKGEVLK
                     SDYRLCQPGAYDWRYHEWAVILPPETTRFNLVLFHHGAGRAWWDDVSLRKAVPAKALG
                     PAQDAVVRDGRPLFQWDEVPGQAVLEIAPEGDFAPDRVRRYPVTGNRFQLPEALPLGK
                     EYSWRVLATGPDGSLRVTLATGADGQPADAARFFAGNWEQRMGELREKLQPYQELLAP
                     LQALARRNRMWDPFKLLAETIAKGEKLATTEPANPREARAELDADLAEIELAAPWWRR
                     IFLDDAALFEGLDLDRPGLEKVKAAVARQEWPAARQALLEYYRARKTPSYYAQYEDPP
                     VRNPAVTT"
     gene            1853..2794
                     /locus_tag="PLY56_12410"
     CDS             1853..2794
                     /locus_tag="PLY56_12410"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22326.1"
                     /translation="MAAWGKPERGARRQSIRSWMAGARLTCPATAIGKGIELPLSPAF
                     LERYVHFIFGQEHPEIDAYLREVAALGVRHVFLRAEEIAELRPEACVPVARVATALAE
                     SRLQVHTVHARFGGPFMLGATRPDGRAEALRVHEETLRAAASLGSDNVVFHLEAAYSP
                     EAEAAARAVLDRLLAVAEAERVRLALEVLSPGCSGSRVEHLSVLLEQYASPWLGICLD
                     FGHAHLAGTLAEWIDAMAAHIFTLHLHDNDGTRDQHQPPGCGTLEWPAMLGRLRRAGY
                     RGPLVSEARTPAGWDSARLIEQFETVCGPWAAPEPEG"
     gene            2831..>3172
                     /locus_tag="PLY56_12415"
     CDS             2831..>3172
                     /locus_tag="PLY56_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HOJ22327.1"
                     /translation="MLQALQERFGIEGAVTFVAGKNGLPTMALRHASGAGAVIYLHGA
                     HVTSWTTPKGEELFFVSRESRFAPGQPIRGGIPVIFPQFGGGELPQHGFARISEWAVL
                     RTGVAEGGEITV"
BASE COUNT          477 a         1050 c         1128 g          517 t
ORIGIN      
        1 gtggtcaccg ccgggttgcg cacgggcgga tcctcatatt gcgcgtagta gctgggcgtc
       61 ttgcgcgcgc ggtagtattc cagcaacgcc tgccgcgccg ccggccactc ctggcgggcc
      121 actgcggcct tcaccttttc caggccgggg cggtcgaggt caaggccttc gaagagcgcg
      181 gcatcatcca ggaagatccg ccgccaccaa ggggcggcga gctcgatctc ggccagatcg
      241 gcatccagct ccgcgcgggc ctcgcggggg ttcgccggct ccgtcgtggc cagcttctcc
      301 cctttggcaa tcgtctccgc cagcagcttg aaaggatccc acatccggtt gcgcctggca
      361 agcgcctgca gtggtgccag cagctcctga tacggctgca gcttctctcg tagctctccc
      421 atgcgctgct cccaattgcc cgcgaagaag cgggccgcat cggcaggctg accatctgcg
      481 ccggtggcca gagtcacccg aaggctccca tccggcccgg tggccagcac gcgccacgaa
      541 tattccttgc ccaggggaag cgcctccggc agctggaaac gattcccggt gaccggatag
      601 cgccggacgc gatccggcgc gaagtcgcct tctggagcga tctccagcac ggcctgcccg
      661 ggcacctcat cccactggaa gaggggccgg ccatcacgca cgaccgcatc ctgtgccggg
      721 cccagcgcct tcgcggggac ggctttgcgc agcgagacgt cgtcccacca cgcccggcct
      781 gccccatgat ggaacagcac caggttgaag cgcgtggtct ccgggggcag gatcaccgcc
      841 cactcatggt agcgccagtc gtaagcgcca ggctggcaga ggcgatagtc gctcttgagc
      901 acctcgccct tcgggcccac cgaggtaatg cgtgccgaag tgcggagctg atgggtgctc
      961 tcgccctcca ctttcaccca cgcggagagg aggtagacgg ccttgggatc ggtctcctcc
     1021 ggcccgaaac cgaccgagag gcccgcgcgg ccctgtgggt cgccatcgat ccgcatagag
     1081 caccggccgg tgcgcgccac ctgcgtgtcg agagccacct gcgagccggt atcccaggtg
     1141 ctaacgcccc atccttccgg ccggccatcg taattggtat ccagctcgaa gctgcggttg
     1201 gcaagtagct cgcctgccgg gcggtcgttt ccggggatcg gctcggtatc gagggtgaag
     1261 ttggcgaggt ggaggactgt ctccggatcg aggcggtgcg cccagttgga gctgaacgcc
     1321 acccaccgga tctggtgcca tcccaccggc tggcgcgcgg atgccatcgc gtcgagcgaa
     1381 acctggaact cgcgccaccc tgtgaagtcg gtcgtgaagg tgaaggagta gtaatccgcg
     1441 ccttcagtct ccgggttccc cgagtcgatc accagggtga tcacggagtc ggtaggcttc
     1501 gcgacgtggg tggcgaagcg gagcgtcttg aaccgggtcc agtcggccgg cgcacccttc
     1561 gcctccacgc gcgtcacgcg cgcgatgtcg ttccaggcga gtgccggttt cccccggtag
     1621 gtcaccgccg ggtcgggggc cagccccgtc cactcatcta gttgcagcgt ctcggcactc
     1681 gccggtgcca ggatcagcag aaacgccagt aaccacagca tcggttcccc tcccgcagcc
     1741 ccacatccgg cgtgggctct tgtgtcgagt ggttcgatag cgtccccccc ggcccctgcc
     1801 gcggacaggc ggaagcggcg gatctatcga aagagagtgg cgggaggtgt taatggccgc
     1861 ctgggggaaa cccgagaggg gcgcccgacg gcagagcatt cgctcctgga tggcgggcgc
     1921 ccggctcacg tgccctgcca cggcgatagg aaagggaatc gagttgccac tctcacctgc
     1981 gttcttagag aggtacgtac atttcatctt tggacaggag caccccgaga tagatgccta
     2041 cctgcgggag gtggccgcgc tgggagtgcg ccacgtcttc ctccgggcgg aggagatcgc
     2101 ggagctgcgt ccggaagcgt gcgtgccggt ggcacgggta gcgacagcgc tcgccgagtc
     2161 ccggcttcag gtgcatacgg tgcatgcccg gtttggcggg cccttcatgc tcggggcgac
     2221 gcgcccggac ggccgggccg aggcgctgcg ggttcacgag gagacgctcc gcgccgcggc
     2281 cagtcttgga agcgataacg tcgtgttcca cctggaggcg gcgtattccc ccgaagcgga
     2341 ggccgccgcg cgcgcggtgc tcgaccgtct cctggcggtc gcggaagcgg agcgggtgcg
     2401 cctggcgctg gaggtgctct cgcccgggtg ctccggctcg cgcgtcgagc acctgtcagt
     2461 tctcctggag cagtatgcct cgccctggct ggggatctgc ctcgatttcg ggcacgcgca
     2521 cctggcgggc acgctggccg agtggatcga tgcgatggca gcgcacatct tcacgctgca
     2581 tctgcacgac aacgatggca cgcgcgacca gcaccagccc cccggatgcg gcacgctgga
     2641 gtggccggcg atgctgggcc ggctccgccg ggcggggtat cgcggcccgc tggtgagcga
     2701 ggcgcgcacg cccgcgggat gggattcggc gcggctgatc gagcagtttg agacggtttg
     2761 cggtccgtgg gccgccccgg agccggaggg gtaggcggca ccgcgagttc ttgggagagg
     2821 agagacgcgc atgcttcagg cactacagga gcggtttggt atcgagggag cggtgacgtt
     2881 cgtcgccggg aagaacgggc tgccgacgat ggcgctgagg cacgcctcgg gcgccggcgc
     2941 ggtgatctac ctgcacgggg cgcacgtcac gtcgtggacg acgcccaagg gagaggagct
     3001 gttcttcgtg agccgggagt cgcggttcgc gccgggccag ccaatccggg gcgggattcc
     3061 ggtcatcttc ccgcagtttg gcgggggcga gctcccgcag catggcttcg cgcggatcag
     3121 cgagtgggcc gtgctccgca ccggcgtggc cgaggggggc gagatcacgg tg
//
LOCUS       DAOSCV010000512         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19628_length_3169_cov_3.063263, whole genome shotgun sequence.
ACCESSION   DAOSCV010000512 DAOSCV010000000
VERSION     DAOSCV010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19628_length_3169_cov_3.063263"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            391..2394
                     /locus_tag="PLY56_12420"
     CDS             391..2394
                     /locus_tag="PLY56_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22328.1"
                     /translation="MKQNQALDDHGQRTQSEASVEGSNAQEVRTGALAKSRFPWRAVA
                     VAAFVIPINTYWVQHSEIVRETAQPTMASLLFNAVYSLILLTAANAWVGRWFPRFRLL
                     RHELLLIYSMVCVASALSGHDQIMILVPLLSYAFHYASPGNLWAEKLHPLLPKHLLVE
                     DRRVIAGYWQGHSSLHTAENLKAWAGPVLWWLAFIVVMAAVMIALGTLFRRRWTHGEK
                     LPYPLLALPIEIVDSGPALWQNRLFWAGFALAGSIDLLNGLHLFFPSLPELKVTAHDL
                     IPVRNTPFLRALGYLPVAFYPWAIGLGMLLPKDFLLSCWVFFWFWKAQGVAGSMLGWS
                     QVPRFPYVPEQAFGAYLGIGLFAIWMARGHLRQVFRWAIAGRGQPGGQEDMSYRLALG
                     VLTVGILFLLLFAIRGGMSPGVALAYFAIYLCLSLAIGRVRGEMGVPSHDLFGGGPDA
                     MLISTLGSRAFTPNTLAMFAVLYWMTRAQRSHPMPHELESLELGHRAGLRGLHVALAI
                     LIAAAIGTLSGIWGALHIGYTNGIAGVPSDSAYFGTEAYGRLDSWLTNPRPADPLAAC
                     AVGFGLGLVLLLLWVRSLFLWWPLHPIGYAITSSISGAILWMPMLIAWVIKAGVLRFG
                     GARAFRSLHPLAMGLILGEFLVGGSWSIVGWILGIKTYRFWSY"
     gene            2774..>3169
                     /locus_tag="PLY56_12425"
     CDS             2774..>3169
                     /locus_tag="PLY56_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22329.1"
                     /translation="MRLLSFFGRFSTTDQMILAIVMVLFLGLIDYLSGWEVSILYLFP
                     IAVVSWHGERWRGIVLSICCAVAWLIADLVQASQPGALPYWNAGVRLGIFLVISLTLN
                     SLRIARERQKELVEFVVHDLRSPLTNVMAG"
BASE COUNT          474 a         1116 c          974 g          605 t
ORIGIN      
        1 ccgcaggagc gcggatgtgc cgggcacccg gcacaaagcc tcggggcttg cgccgcccac
       61 tcgccacggc tacccgcgac gagggagcgc gatacaccgc ctgacggtct ggcccggatc
      121 cgctctggag gagccgtcca gggagcaaaa cgcaaaccct tcccagcaca gcgggccagc
      181 ggtaccccgc gacttacggg aggtcactcc cctgccccag atcacccgct gcgccctggt
      241 gagaccacgg ccctcagtag cagaaccagg gagtgccttt tcgcaaggag atagcaccgt
      301 caaaaccgga gaggaggccg ggagttgcag ggggctctcc atcccccgcc caaagacggg
      361 gagccctcac tcctgcaccc ctgaatccat atgaagcaga atcaggcgtt ggacgaccac
      421 gggcagagga cgcagtcgga agcgtcggtc gaaggaagca acgcgcaaga ggtgagaacg
      481 ggcgctttgg cgaagagtcg ctttccctgg cgagccgtcg ccgttgccgc gttcgtgatc
      541 ccgatcaata cctactgggt gcagcactcc gagatcgtgc gcgagacggc ccagcccacg
      601 atggcgtcgc tcctcttcaa cgccgtctac tcgctcatcc tcctgaccgc ggcgaacgcc
      661 tgggttgggc ggtggttccc gcgcttccgc ctcctccgcc acgagctttt gctcatctac
      721 tccatggtct gcgtggcgtc tgccctctcc gggcacgacc aaatcatgat cctcgtgccc
      781 ctcctgagct acgctttcca ctacgcctct ccgggcaatc tgtgggccga gaagctgcac
      841 cccctgctcc cgaagcacct tctcgtagag gaccgccgag tgatcgcggg ctactggcag
      901 ggccactcca gcctgcacac cgccgagaat ctgaaggcat gggccgggcc cgtgctgtgg
      961 tggttagcct tcatcgtggt catggcggcg gtgatgatag ccctcggcac gctcttccgc
     1021 cggcgatgga cgcacggcga gaagctcccg tacccccttc tcgcgcttcc catcgagatc
     1081 gtcgactccg ggccggccct ctggcaaaac cgcctcttct gggccggctt tgcgctcgcc
     1141 ggctccatcg atctcctcaa cggcctccat ctcttcttcc cgtcgctccc ggagctgaag
     1201 gttaccgccc acgatctcat ccccgtgcgc aacaccccct tcctgcgtgc cttgggctac
     1261 ctgccggtcg cgttctaccc ctgggccatc ggcctgggaa tgctccttcc aaaagacttc
     1321 ttgctctcct gctgggtctt cttctggttc tggaaggcac aaggagtggc gggatcgatg
     1381 ctcggctggt cacaagtgcc ccgattcccg tatgtgcccg agcaggcgtt tggcgcctat
     1441 ctggggatcg gtctcttcgc catctggatg gcgcgaggcc acctgcgcca ggtgttccgc
     1501 tgggccatcg cgggccgcgg ccagcccggc ggccaggagg atatgagtta ccgcctggcg
     1561 ctcggcgtcc tgacggtcgg gatcctcttc ctgctcctct tcgccattcg gggtggcatg
     1621 tcgccggggg tcgcgctcgc ctatttcgcg atctacctct gcctgtcgct cgccatcggc
     1681 cgggtccgcg gcgagatggg cgtgccatcg catgacctct ttggcggcgg gccagacgcg
     1741 atgcttatct ccacgcttgg ctcgcgcgcc ttcaccccca acacgctggc gatgttcgcc
     1801 gtcctctact ggatgacgcg cgcccagcga tcgcacccga tgccgcacga gttggagagc
     1861 ctagagctgg ggcatcgcgc cggcctgcgg gggcttcacg tcgccctggc catcctgatc
     1921 gccgccgcga ttggcacgct gtctggcatc tggggggcgc tgcacatcgg ctacaccaac
     1981 gggattgccg gcgtgcccag cgattcggcc tatttcggca cagaagccta tggccggctc
     2041 gattcctggc tcaccaaccc ccgccccgcc gatccgctgg ccgcctgcgc cgtcggcttc
     2101 gggctcgggc tcgtgctcct cctcctatgg gtgcgcagcc tgttcctctg gtggccactc
     2161 cacccgatcg gctacgccat caccagcagc atctccggcg ccatcctctg gatgccgatg
     2221 ctcatcgcct gggtgatcaa ggccggcgtc ctgcggtttg gcggcgcccg cgccttccgc
     2281 tcgctccacc cgctcgcgat ggggcttatc ctgggcgagt tcctggtggg cggctcgtgg
     2341 tcaatcgtgg gctggatcct ggggatcaag acctaccgct tctggtcgta ctagggcatc
     2401 acggggcccc tctcccctgc ctctcggcat tgtaggaagg ggcccgtctc cgccagctgt
     2461 gctgcctgcc atccctcgcc tggccacgcc gggagtgagc gcagtccggt gccaggccgc
     2521 ggtgaccggc cagatgcccc cgcagcgcac gcatccatcc aaagaagaat cttcctgaaa
     2581 gaactgggct cgctctgccg aaataggagg aaaagccgcc agtggtgatg cacggaaacg
     2641 cgatgaggcg gctctcatca cgggcaggcg ccgggcctcg cgaggcgcct ggcaccggat
     2701 ggcgtctcgg gcgcgaccag gcggtaaggg aaacgccgga gccgcgctcc caggaaaagg
     2761 caaaacacgc gatatgaggc tgctatcatt ctttggccgg ttttcgacca ccgaccagat
     2821 gatccttgcc atcgtgatgg tcctcttcct cggcctgatc gattacctct cgggctggga
     2881 agtctcgatc ctctacctct tcccgattgc cgtggtctcc tggcacggcg agcgctggcg
     2941 gggtatcgtc ctctcgatct gctgcgcggt ggcctggctc atcgcggatc tagttcaggc
     3001 atcccaaccc ggggcgctcc cctattggaa cgccggtgtg cgcctgggga tcttcctcgt
     3061 catctcgctc actctcaact cactgcggat cgcgcgcgag aggcagaagg agctggtcga
     3121 gttcgtcgtc cacgaccttc gttcgccgtt gacgaacgtg atggcgggg
//
LOCUS       DAOSCV010000513         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19633_length_3168_cov_3.786058, whole genome shotgun sequence.
ACCESSION   DAOSCV010000513 DAOSCV010000000
VERSION     DAOSCV010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19633_length_3168_cov_3.786058"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2943)
                     /locus_tag="PLY56_12430"
     CDS             complement(<1..2943)
                     /locus_tag="PLY56_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22330.1"
                     /translation="MVRKSFIWLSLAVAASIACPPADAQRAGTGNRSNVPFSSVADGM
                     LGPVIVDVPAGASRQAIDQAVATTWQRGKPNAATGAAIHRFLTRWKLIRPGTQLSFHR
                     PVILRENGRLRLPELDRVRQTRSRLGGGEITFVYEGWSPQDAETLRAFVDLMYPIAKQ
                     VYGNPSATIKVRIVRDDLLRDSAMHLRNGIYNASTNEIRFNPTGNLVDDQYSLTHLIL
                     YAFHDDALFAYDAWEEGFSWAATYVIFKHIRPDYDLGESFKTFYLMQLYELLNQPGIG
                     NARFWPANYDSIVQNEYAAVFPYSMFVFRSAMAGAAWAKVYVENNAFFREFNAAYYQQ
                     YTPELAGHTPTLKAIASRIVPTVEGQDFFQWYRRQHILDTSTAAGRHFWVYALPEFNN
                     DESSAYNVLLLYHFTRDAAGNEVPQGGTARFTYYSWDFLYDYYAEEGNEAVIPGSGDS
                     IGVGALSPGVYNVGGPQKINIQASVNGLTRMVYWPYLVRGLETAPNDLYGVTLDTEAG
                     EVGVIVGTGVEQKVPLSQAAFGMNAGLNGWHKTTIRVTDSAGQTVTRQVNTGYDSYAA
                     VVQGPRPAGATYTLTLPAGAHMISVPAYPKVSDAEALGVPAQRLRLAHWDPTLSTGDP
                     GYRYRLYPATPGLAPGIGYWLILDQQTTINFEGNPLPHDQSYLIPLRAGWNQIGHPFT
                     QPVAIDSLSVAMSPSDPEQVITLQAAVQQGKLSGTFWQYTQQQGYTAATQLEPWRGYW
                     VYAESDLLLKVPAVASPGQNPPGALLPGDPVIGDVRSRARRASTPSRQEISSPASTRS
                     RQEDGWRLRLAARVRGQAATLTLGASRSATDAFDPLHDAQRPPEMGDYVRLSVPHSDW
                     GRAAGDYAIDIRADGAARYTWDIVLETSLRNEEVQLSWPDLASLPRDMRITLIDLDGG
                     KRRYLRTTGSLVINSGETGRRRLRVEATRGGAGLLAINNITVTPTRATGALDVGFSLT
                     SDALLDAQI"
     gene            complement(2957..>3168)
                     /locus_tag="PLY56_12435"
     CDS             complement(2957..>3168)
                     /locus_tag="PLY56_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22331.1"
                     /translation="QVFANYPDRFVDPIWEAGSLAASGTSVNYTGPMLQPGHTYYWVV
                     LARNSGDPETITGWSISRITPFTIP"
BASE COUNT          551 a          981 c         1089 g          547 t
ORIGIN      
        1 gatttgggca tcgagcaggg catcggaggt gagcgagaag cccacatcca gggcacccgt
       61 ggcccgggta ggcgtcacgg tgatgttatt gatcgccagg agcccggccc cgccacgggt
      121 cgcttccacg cgcaggcggc gccggcctgt ctcgccggag ttaatgacga ggctgccggt
      181 agtccgcagg tagcggcgct taccgccatc gaggtcgatg agggtgatcc gcatatcgcg
      241 cggcagcgag gcaagatccg gccaggaaag ctggacctcc tcgttgcgca ggctcgtctc
      301 cagcacgatg tcccacgtgt agcgcgccgc gccatccgca cgaatgtcga tggcgtagtc
      361 gccggccgcc cggccccaat ccgaatgggg cacgctcaag cgcacgtagt cgcccatctc
      421 gggcgggcgc tgggcatcat ggagcgggtc gaaggcatcc gtcgcgctcc gggatgctcc
      481 cagggtgagc gtggccgcct gaccgcgcac acgggccgcc agccgcaacc gccacccgtc
      541 ttcctggcgc gatcgcgtgc ttgcaggcga agagatctcc tgacgagaag gcgtgctcgc
      601 ccggcgggcc cggctgcgca catcgccaat caccggatcg cccggcagca gggcgccagg
      661 cggattctgg cccggagagg ctaccgcggg gaccttcagg agaagatccg actccgcata
      721 gacccaatag ccgcgccagg gctccagttg ggtcgccgcg gtgtatccct gttgctgcgt
      781 gtattgccag aaggtgccgc tcagtttccc ctgctgaacg gccgcctgca gcgtgatcac
      841 ctgctcggga tccgagggcg acatcgccac gctgagcgag tcgatcgcga ccggctgcgt
      901 gaagggatgg ccgatctgat tccagccggc acgcaggggg atcaggtacg actggtcgtg
      961 gggcaggggg ttcccctcga agttgatcgt cgtctgctga tcgaggatca gccagtagcc
     1021 aatgcctggg gcgagacctg gcgttgccgg gtagagccgg tagcgatagc ccgggtcacc
     1081 cgtggagagc gtcggatccc agtgggcaag gcgcaggcgt tgggcaggca cgcccagtgc
     1141 ctccgcatcc gataccttcg ggtacgcggg aacggagatc atgtgcgcgc cagccggcag
     1201 cgtcagcgtg taggtcgcgc ccgctgggcg tggcccctga acgacggccg cataggagtc
     1261 gtagcccgtg ttcacctgcc gggtcaccgt ctgcccggcg gaatcggtga cgcggatcgt
     1321 cgtcttgtgc cacccgttca gacctgcgtt cataccaaag gcggcctgag agaggggcac
     1381 cttctgctcc acaccggtgc cgacgatcac gcccacctcg cccgcctcgg tatccagggt
     1441 gacgccatag aggtcattgg gtgcggtttc gaggccgcgc accaggtagg gccagtagac
     1501 catgcgtgtt aggccgttga ccgatgcctg aatgttgatt ttctgcgggc cccccacgtt
     1561 gtagacgccc gggctcagcg cgccaacccc gatgctatcg ccgctgccgg ggatcaccgc
     1621 ttcgttgccc tcttccgcat agtagtcgta gaggaaatcc cagctgtagt aggtgaagcg
     1681 ggcggtcccg ccctggggca cctcgttccc cgcggcgtcg cgggtgaagt ggtagaggag
     1741 aagcacgttg taggcgctgg actcgtcgtt gttgaactcc ggcagcgcat acacccagaa
     1801 atggcggccc gctgcggtgg atgtatccag gatatgctgg cgccggtacc actggaagaa
     1861 atcctggccc tcgacggtgg gcacgatcct ggatgcgatc gccttcaggg tcggggtgtg
     1921 ccccgccagt tcgggcgtat attgctggta gtaggccgcg ttgaactcgc ggaagaaggc
     1981 gttattctcg acgtacacct tggcccacgc agcgcccgcc atggccgagc ggaacacgaa
     2041 catcgagtag gggaagaccg ccgcgtactc gttctggacg atcgaatcgt agttggccgg
     2101 ccagaagcgc gcgtttccga tgcccggctg gttcaacagc tcgtaaagct gcataagata
     2161 gaacgtcttg aacgattcgc ccaggtcgta gtccggccgg atatgcttga agatgacata
     2221 ggtggccgcc caggagaaac cctcctccca cgcgtcgtag gcaaagagcg catcgtcgtg
     2281 gaaggcgtag aggatcaggt gggtgaggct gtactggtca tccaccagat tgccggttgg
     2341 gttgaagcga atctcgttgg tggacgcgtt gtagatgcca ttgcgcaggt gcatcgcgga
     2401 gtcgcgaagc aggtcgtcgc gcacgatgcg caccttgatc gtggccgagg ggttgccgta
     2461 gacctgcttg gcgatgggat acatcagatc cacgaacgcg cgaagcgtct cggcatcctg
     2521 cgggctccag ccttcgtaga cgaaggtgat ctcgccgccg cccaggcggc tccgggtctg
     2581 gcgcacgcga tcgagctccg gcaggcggag acggccattc tcgcgcagga tcaccggccg
     2641 gtgaaagctg agctgggtgc ccggccggat cagcttccag cgcgtgagga agcggtggat
     2701 ggccgctccc gtcgccgcgt tcggtttgcc gcgctgccag gtcgtggcca cggcctgatc
     2761 gatcgcctga cgcgatgcgc ccgcgggcac atcgacgatg acgggcccca acatcccatc
     2821 ggcaaccgat gagaagggca cgttggagcg gttgccggtg ccggcccgct gggcatctgc
     2881 cggagggcac gcgatggagg cggcgaccgc cagggagagc cagatgaagc tctttcggac
     2941 caagatagac ctcccgctat ggaatcgtga atggcgtgat tcggctgatc gaccaccccg
     3001 tgatcgtctc cggatcgccg ctattccggg ccagaaccac ccagtagtag gtgtgccctg
     3061 gttgcagcat cggacccgta tagttgaccg acgtcccgga ggcggcgagt gatccggctt
     3121 cccagatggg atcgacgaag cggtctggat agttggcgaa aacctgaa
//
LOCUS       DAOSCV010000514         3160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19701_length_3160_cov_3.667311, whole genome shotgun sequence.
ACCESSION   DAOSCV010000514 DAOSCV010000000
VERSION     DAOSCV010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_3160_cov_3.667311"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1872
                     /locus_tag="PLY56_12440"
     CDS             286..1872
                     /locus_tag="PLY56_12440"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ22332.1"
                     /translation="MAKRKQRILVVDDEVGNQEVLQELLEILGYETIAVGDGVEALEA
                     VAERRPDLIILDVMMPRMDGFEVCRRLKSAPETAFIPIIIITALGDLSHRIEGIKAGA
                     DDFLTKPFSQAELSARANALLRSKRLHDDLENAYRTMERLARYTEGILNAFDPLRFDK
                     DVMEESLVGQLLESTYATGRSPQAVFVGSIDGSDLPFGNLYIAEGTRVHRSERMIALE
                     MPGRITRGSPETMTFSNCPDGTDLASYQEGFSPDLRQILGVIRNCAAVLIGRTYLAAF
                     NFEEDVSFSEAVALKGFGVHVGFLETIAEQIQETERGFLYTIGALARAAEANDEDTGD
                     HILRVNEYSRVLAETLGLPQNDTEAIHYSAQMHDVGKIHVHPDVLRKRGRITPEELEV
                     MRRHTIAGARILGDAPRLRVAASIALCHHEHWDGTGYPQGLKGEQIPIAARIVTVADV
                     YDALRMARSYKPAHSHEDACRIILKGDGWTVPEHFDPAVLAAFEKTQAQFEEIYRQMA
                     GNGSSARKETWLLHGAPVLP"
     gene            2067..>3160
                     /locus_tag="PLY56_12445"
     CDS             2067..>3160
                     /locus_tag="PLY56_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOJ22333.1"
                     /translation="MRQDRIWLGAISLFLGLAVAVVPAASEEPAVEDEEEVVVTAQRR
                     KETRTEVPASLSVITRREIERSNAQNLVDLLVREPGVFVSRQGGLGYGGSIQVRGLGG
                     NPPVGSLVLIDGHPDFMGIMGHSMPSAYLVDHVERVEILRGPASTLYGTNAAGGLIHI
                     VTRRPEGEGASAQVWAETGSFGTRNMQGRVGTAMGRFSAAFMAGHRETDGHLPNGYGW
                     YQASNYALNAWQQWSPRVSTRFTGSVVRYDTIDQRALATAYWAGTPIPRLRQEFNRRD
                     FGVTTDWTGERRDSTLSLHYNPSEHRFEDGFHSTDFVGGLSWIQRFHGSETIRGAWGL
                     DLQRYGGEVKSPAPSAGHFARSEGGVYATV"
BASE COUNT          575 a          976 c         1063 g          546 t
ORIGIN      
        1 gcggccaacc aattgatcgt aacacacccg gtagggcatg tcaacgcccg gcggccgcgc
       61 gacgccaccg accttccggt cccgaagggg gcgcattgcc tggctcttct atcgttccga
      121 ttccagccgt aggaggagcg ggcgttcgcg tcgaaggatc acacagtcga gtaggcgttg
      181 ataaggcgcg cgccgagaga gcggcgtgct cgcaggtcgc gacaggcgta ggaagcgcgg
      241 acctcttcac ctggctgacg gatggcgggt ggcgcgggaa cagcgatggc gaaacgcaag
      301 cagaggatcc ttgtcgttga tgacgaggtg gggaatcaag aagtgctcca ggagctgctg
      361 gagatcctgg gctatgaaac gattgccgtg ggagacggtg tcgaggcatt ggaggccgtg
      421 gcggagcggc gcccggatct gatcatcctc gacgtgatga tgccgcggat ggatggcttc
      481 gaggtgtgcc ggcggctcaa atccgccccg gagaccgcgt tcatccccat catcatcatc
      541 acggcgctgg gtgacctctc tcaccggatc gaagggatca aggccggcgc cgacgacttc
      601 ctcaccaagc cattcagcca ggcggagctc tccgcgcgcg cgaacgcgct gctgagatcc
      661 aagcgcttgc atgatgacct ggagaatgcc tatcggacca tggagcggct ggcgcgctac
      721 accgagggca tcctgaacgc gttcgatccc ctccgctttg ataaggacgt gatggaggag
      781 tcgctcgtcg ggcagttgct ggagagcacc tacgcgaccg gccgatctcc gcaggcggtc
      841 ttcgtaggca gcatcgacgg ttccgatctc ccgttcggca acctctacat cgccgagggg
      901 acgcgcgtgc ataggagcga gcgcatgatc gccctggaga tgcccggccg gatcacccgg
      961 ggctccccgg agacgatgac gttctcgaac tgcccggatg gaacggatct cgcatcgtac
     1021 caagaggggt tctctcccga cctgcgacag atcctcgggg tgatccgaaa ctgcgccgcg
     1081 gtcttgatcg gccgcaccta cctggctgcc ttcaactttg aggaagatgt ctccttctcc
     1141 gaggcggtag cgctcaaggg gttcggtgtc cacgtagggt tcctggagac gattgccgag
     1201 cagatccagg agacggagcg tggcttcctc tacaccatcg gagcgctggc gcgcgccgcg
     1261 gaggccaacg acgaggatac cggcgaccat attctgcgcg tgaatgagta ttcccgcgtg
     1321 ttggcagaga ctctgggcct accccagaat gataccgaag cgatccacta ctcagctcag
     1381 atgcacgacg tgggcaagat ccatgtccat cccgatgtcc tgcgcaagcg ggggcggatt
     1441 acgcccgagg agctcgaggt gatgcgtcgg catactatcg ccggtgcccg gatcctgggc
     1501 gatgcgccgc gcctgcgagt ggccgcttcc atcgcgctgt gccaccacga gcactgggat
     1561 ggcaccggct atccgcaggg cctcaaaggc gagcagatcc ccattgccgc gcgtatcgtc
     1621 accgttgccg atgtctacga tgcccttcgc atggcacgca gctataagcc cgctcactcg
     1681 cacgaggacg cgtgtcggat catcctcaag ggcgatggct ggaccgtccc cgagcatttc
     1741 gaccccgcgg ttcttgcagc gttcgagaag acacaggcac agttcgaaga gatttaccgc
     1801 caaatggccg gcaatggaag cagcgcgcgg aaagagacct ggctcctcca cggagcccct
     1861 gttcttcctt agccatcctt gctcgcgcct ccagaaaacc ctatccgagg catcccgatc
     1921 caccaggatc gggcgcgcag catgccctcc cgctcctcac agtggtgcct ccttgacgtg
     1981 cctgtgcctg gctgctataa tgacgcggtg ttacggtttg agaaaatcgt gttacgccgg
     2041 actcggatag gacaggaggc aggagaatga gacaggatcg catctggctc ggagccatct
     2101 cgctgttctt ggggcttgcc gtggcggtgg tgccggcggc gagcgaggag ccagcggtag
     2161 aggatgaaga agaggttgtg gtcacggcgc aacgccggaa agagacgcga acggaggtgc
     2221 cggcgagcct gagcgtgatc acgcggcgag agatcgagcg ttcaaacgct cagaacctgg
     2281 tcgacttgct ggttcgcgag ccgggcgtct tcgtgagccg ccagggcggg ctgggctacg
     2341 gcgggagcat tcaggtgcgg ggtttggggg gcaacccgcc ggttggcagc ctggtcttga
     2401 tcgacggcca ccccgatttc atggggatca tgggccactc gatgccaagc gcctacctgg
     2461 tggatcacgt ggagcgggtg gagatcctgc gcgggccggc ttcaacgctc tatggcacca
     2521 acgccgccgg agggctgatc catattgtga cgcgccgccc ggaaggggag ggggcttcgg
     2581 cgcaggtgtg ggcagagacc ggctcgtttg gcacgcgcaa catgcagggg cgcgtgggga
     2641 ccgcgatggg ccggttctcg gccgcgttca tggcggggca tcgcgagaca gacggacacc
     2701 tgcccaacgg ctatggctgg tatcaggcat cgaactacgc gctgaacgcc tggcaacagt
     2761 ggtcgccccg cgtctcgacg cgcttcaccg gttcggtagt ccgctacgac accatcgacc
     2821 agcgcgcgct ggcgaccgcc tactgggccg gcacccccat cccgcgcctg cgccaggagt
     2881 tcaatcgccg cgatttcggg gtcacgaccg actggaccgg cgagcggcgt gattccacgc
     2941 tctcgctcca ctacaacccc agcgagcacc gatttgagga cgggtttcac tcgaccgatt
     3001 tcgtcggcgg gctctcctgg attcagcgct ttcacggttc ggagacgatc cggggggcat
     3061 ggggcctgga cctgcagcga tacgggggcg aggtgaaaag cccggctccc agcgccggcc
     3121 acttcgcccg cagcgagggc ggcgtctacg cgaccgtgga
//
LOCUS       DAOSCV010000515         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19709_length_3159_cov_3.478737, whole genome shotgun sequence.
ACCESSION   DAOSCV010000515 DAOSCV010000000
VERSION     DAOSCV010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19709_length_3159_cov_3.478737"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3159
                     /locus_tag="PLY56_12450"
     CDS             <1..>3159
                     /locus_tag="PLY56_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOJ22334.1"
                     /translation="PSRAPVEAESYDYTPKTRQVKACILLLDSFKVNGQPENPDPHVF
                     YLLDSEAKGTAKGLKPRDWEIVNPLAPRIVTPDIKARWDAIDPSAASRPDFQINSPVT
                     KEMACYWEVPLSSTPLEDLLQFDLIAVMTHAQGGFSLEDSAKLRRLVDLGVTLWFDDC
                     AGARIDPNRPFFIRDFNFDSSVKPASTPDPTLVPADRFHPLLNSPFALTTQELEQLGD
                     KGADHFTYRITNLAQSGPPSPLLAPIVNSNGDFGDGFTYPVIAGGRYGDGVVLVTSGD
                     IACAINDRFNAGPNAADANSGAFCGDLPAAWDESAQNRLESNMKFAFNVLNWTGGESN
                     YRKGPHQTAHMRDEVSPGLDTAWKVPLGSSVRLLGPPVVWRDFAFFTIVENQGAVLGS
                     RIFAVDMRPYEDRDGRDENGNPRDGLGNPNDGMEGGWVDAGGTPIVDLTPESPYDVIW
                     RYDLPGIVASEPYVATVVVNQQPVDAVFFTTRDGLLHAVRAIPTVPNADGEPVLASNT
                     AELPGYPMTIATDPNEITAPVVSREYVYVLTVEAGYYCVNVVAAATGVGKYKVPIGAA
                     SGVCTLATPAVVLMGNEERGSTTEVAYASLPPGQLMALATYTYDERADRLPNDPERFQ
                     LQHAWRRDPAWSAPDPVPPTTLKVTVLDTLGYPVGPTNPNPSNRWTYRVDDEDQDRVI
                     QVRRQMDETTLVPIPEGFRVIVDYEIDPSRPGGMRTPWTFTPGYPAATPLVQSGAAVA
                     SNDSVVYASANGFVQMLAERGARDPNELLWRCNVNDYVADGTDPSGLLPTFRRSSFTS
                     TDGTTYEEITWIPAWWDDARGWIPLQPAVTDDMVYIAMNRYALKVNGTELTHITGYDD
                     GLIEGAIFAFERWDTMEVDLGDVTGADLSKLTVMTYLPEVGSWTESTQEQWVGNPNTY
                     WRLKTHTVGGRTYARIIFERFPNELSLMSSLWYDDADADALPNNLCSPIWVDLDGPGT
                     AVPRPYAVNGRKLSNLAWRYPDPEWHGVPPAEGTGPRAERALWLEGVTSSPTVSGGVV
                     SVAEGDGRLFALDADPRQGARGA"
BASE COUNT          564 a         1024 c         1017 g          554 t
ORIGIN      
        1 ccatcgcgcg cgccggtgga ggcggagtcg tatgactaca cccccaagac gcgccaggtg
       61 aaggcgtgta tcctgctcct cgactccttc aaggtgaatg ggcagccgga gaaccccgac
      121 ccgcacgtct tctacctgct cgattctgag gcgaagggta cggcgaaggg cctaaagccg
      181 cgcgactggg agatcgtcaa tccgctcgcg ccgcgtatcg tcacgccgga tatcaaggcg
      241 cggtgggatg ccatcgaccc cagcgcggcc tcgcggccgg atttccagat caacagtccg
      301 gtgacgaagg agatggcgtg ctattgggaa gtgcccctct ccagcactcc cctggaggac
      361 ctgctccagt tcgacctgat cgccgtgatg acacacgccc agggtggctt cagcctggaa
      421 gatagcgcca agctgcgccg gcttgtggac ctcggcgtga ccctctggtt cgatgattgc
      481 gccggtgccc ggatcgatcc aaaccggccc ttctttatcc gcgacttcaa ttttgattcc
      541 agcgtgaaac cggcgtccac gcctgacccc accctggtgc ccgcggatcg cttccacccc
      601 ttgctcaaca gccccttcgc gctcaccacg caggagctgg agcaactggg cgataagggc
      661 gctgaccatt tcacgtaccg catcaccaat cttgctcaga gcgggcctcc cagccccttg
      721 ctggcgccta tcgtcaacag caatggcgac ttcggcgatg gcttcaccta cccagtgatc
      781 gcggggggcc gctatggcga cggcgtcgtg ttggttacct ccggcgatat cgcctgtgcg
      841 atcaacgacc ggttcaacgc cggcccgaac gcggcagacg ccaacagcgg agcattctgt
      901 ggcgacttgc ctgccgcctg ggacgagagc gcgcagaacc gcctggagag caacatgaag
      961 ttcgccttca acgtcctgaa ctggacgggg ggcgaatcga actatcggaa gggccctcac
     1021 cagacggccc acatgcgcga tgaggtctcg cctgggctcg atacggcctg gaaagtgccg
     1081 cttggatcaa gcgtgcgcct gctggggcca ccggttgtgt ggcgcgactt cgctttcttc
     1141 accatcgtgg agaaccaggg ggctgtcctg ggcagccgta tcttcgcggt tgacatgcgc
     1201 ccctatgagg accgcgacgg ccgcgatgag aacggcaacc cgcgagacgg cctgggcaac
     1261 cccaacgatg ggatggaagg cggctgggtt gatgctggcg gcacgccgat tgtcgacctc
     1321 acgccggaaa gcccctacga tgtgatctgg cgttacgatt tgccgggcat tgtcgcctcg
     1381 gagccctacg tggccacggt ggttgtaaac cagcagccgg tagatgctgt cttcttcacc
     1441 actagagacg ggctgcttca tgctgtccgt gcgatcccaa ccgtccccaa tgccgatggc
     1501 gagcctgtgt tggcctcgaa cacggcggag ctgccgggct atccgatgac gattgccacg
     1561 gacccgaacg agatcaccgc gccggtggtg agcagggagt atgtttacgt cctcactgtc
     1621 gaagcgggct attactgtgt gaacgtggta gctgccgcga ccggtgtcgg caaatacaag
     1681 gtacccattg gggctgccag cggtgtatgc actctcgcca cgccggctgt ggttctgatg
     1741 gggaacgagg agcgggggag cacgaccgag gtggcctacg cctcgctgcc gccaggccag
     1801 ctgatggcgt tggctaccta cacctacgac gaacgagcgg atcgcctccc caatgacccg
     1861 gagcgattcc agctgcagca cgcctggcgg cgcgatcccg cgtggagcgc gccagacccg
     1921 gtgccgccga cgactctgaa ggtgaccgtg ctggatacgc tgggctatcc cgtcgggccg
     1981 accaatccca acccgagcaa ccggtggacc taccgcgtgg atgacgagga ccaggaccgc
     2041 gtgatccagg tgcgccgcca gatggatgag acgacgctgg tcccgatccc cgaaggcttc
     2101 cgcgtgatcg tcgattacga gatcgatccc tctcggccgg gcgggatgcg cactccctgg
     2161 acgtttacgc cgggctatcc agcggcgacg ccactcgtgc aatcgggtgc cgcggtggcc
     2221 agtaacgata gcgtcgtcta cgcctcggcg aacggctttg tgcagatgct cgccgagcgt
     2281 ggcgcgaggg atcccaacga actcctctgg cgatgcaacg tcaacgacta cgtggcggat
     2341 ggcaccgatc ccagcggtct cctcccaacg ttccggcgga gctccttcac ctccacggat
     2401 ggtaccacgt acgaggagat cacctggatc ccggcgtggt gggacgacgc tcgcggctgg
     2461 attccgctgc agcctgcggt gaccgacgac atggtctaca tcgccatgaa ccgctacgcg
     2521 ctgaaggtga atggcacgga gctgacccac atcaccgggt acgatgatgg cctcattgaa
     2581 ggcgctatct tcgccttcga gcgctgggac accatggagg tggacctggg tgacgtcacg
     2641 ggcgcagacc tctcgaagct gaccgtgatg acgtatctcc ccgaggtggg aagctggacc
     2701 gagtcgacgc aagagcagtg ggttggcaat cccaacacct actggcgact gaagactcac
     2761 acggtgggtg gccggacgta cgcgcggatc atcttcgaac gcttcccgaa cgagctgtcg
     2821 ctgatgtcga gcctgtggta tgacgatgcc gacgcggatg ccctcccgaa caacctctgc
     2881 tcgcccatct gggtggatct tgatggtccg ggcacggccg tgccccggcc atacgcggtg
     2941 aatggcagga agctctccaa cctggcctgg cgctatcccg atccggagtg gcacggcgtg
     3001 ccgcccgccg aaggcaccgg accgcgcgcg gaacgcgcgc tctggctcga gggcgtgacc
     3061 tcttccccaa cggtctccgg gggcgtcgtt tccgtggccg agggagatgg ccggctcttc
     3121 gccctggacg cggacccgcg ccagggggca cgcggtgct
//
LOCUS       DAOSCV010000516         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19711_length_3158_cov_3.473091, whole genome shotgun sequence.
ACCESSION   DAOSCV010000516 DAOSCV010000000
VERSION     DAOSCV010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19711_length_3158_cov_3.473091"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PLY56_12455"
     CDS             <1..1512
                     /locus_tag="PLY56_12455"
                     /inference="COORDINATES: protein motif:HMM:NF018228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ22335.1"
                     /translation="LAIDHAHLQEQVMHAERMKALGEMSAGVAHDLNNLLAAIMGNAQ
                     LALMNAKDVNTVSTAMETISRVCLEGAETIRRIHSITGRPHHSPGQLIDVADVIRQAV
                     QATRAHWNDRPSGQEVQVMLDLEAGIQVHGCAADLQEAIAHLITNAVDAMPEGGKLTL
                     RCLSQHPWAVLEVIDTGLGMEDAVRQRVFDPFFTTKAQRGHGLGLSMVYGIVHQHLGT
                     IVVESAVGRGSTFTIRIPLATSMRAPSLSPAASLPKLRVLVVDDESLVAETLAGILRC
                     LGHSVDTAPGGEEALEIWRADAYDLVITDHGMPGMTGTELAQRLKEQAPNVPVLLITG
                     WDIHPGVTTNTHEVVDAVLQKPISREELLRGITSALHATAVRAAQANETRRVLVVDDH
                     PAITAVIDGALSSSGYKVECCTDADAAIARLEEALPDLVITDYHLEGTSGIDLARVVK
                     EKNPGVPVILITGWRPDKHELRDIDLCLNKPFNVRELLDAVASLAKDGPRAPK"
     gene            complement(1571..2092)
                     /locus_tag="PLY56_12460"
     CDS             complement(1571..2092)
                     /locus_tag="PLY56_12460"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOJ22336.1"
                     /translation="MADSQAAAGPAGYRNAPPFLMIVIAFCSAASLGVSTLALLNARA
                     RDVATPGGGGAAHAAHGSAEERTRHELGEFLVNLDEPDPQAFVKTTIVVEYAGGKKSG
                     EQKEGKEKAPEWEVPVRDVIVSTLSQCRRADLRSEKGKEQLKKLLLRRLNQTDDPSAP
                     KFLAVYLTSFAMQ"
     gene            complement(2148..2924)
                     /locus_tag="PLY56_12465"
     CDS             complement(2148..2924)
                     /locus_tag="PLY56_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HOJ22337.1"
                     /translation="MDLATAIGLLLAFGCLVLSVCMDGGNLAAFVNVPAAVLVFGGTI
                     GATIICFPLSKILKLPAIARNAFLNRELNRAQMIDTMVGFAQKARREGLLVLEDDAAR
                     VDHALLRMGVELAVDGTPQERVRDILTIEIDAMHERHRQGEELFATMGAVSPKLGVIG
                     TLIGVIHMLGAQTDPKQMGPALASAFIATLYGVAFANLVALPIAAKLRARGEEEAMFH
                     QMILEGLMAIQSGDNPRAVEMKMRAFLSPEQKMATRAREA"
     gene            complement(3007..>3158)
                     /locus_tag="PLY56_12470"
     CDS             complement(3007..>3158)
                     /locus_tag="PLY56_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009676750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HOJ22338.1"
                     /translation="IVSGALEISNVDLTREFSSLIVYQRGFQANGRSVTVSDQLLQDI
                     LMLKQ"
BASE COUNT          594 a         1047 c          950 g          567 t
ORIGIN      
        1 cttgctatcg accacgctca cctgcaagag caggtaatgc atgccgagcg gatgaaggcg
       61 ctcggtgaaa tgtccgcggg cgtcgcccat gacctcaaca atctccttgc ggcgatcatg
      121 ggcaacgctc aactggcgct gatgaacgcc aaagacgtca atacagtctc taccgccatg
      181 gaaacgatct cacgcgtctg cctcgagggc gccgagacga tacggcgcat ccattcgatc
      241 acgggccggc cccatcacag ccccggccaa ctcatcgatg ttgctgatgt catccggcag
      301 gccgttcagg ccacacgggc acattggaac gaccgcccgt cgggccagga agtgcaggta
      361 atgctggatc tcgaggccgg catccaggtg catggttgcg cggccgatct ccaggaggcc
      421 attgctcacc tgatcaccaa cgcggtcgat gcgatgcctg aggggggcaa actcaccctc
      481 cggtgcctgt cgcagcaccc ctgggccgtt ctagaagtca tcgataccgg cctcggcatg
      541 gaggatgcgg ttcgccagcg tgtcttcgac cccttcttca ccacgaaagc acagcgcggt
      601 cacggcctgg gtctgagcat ggtgtatggg atcgtacatc aacacctggg aactatcgtg
      661 gtggaaagcg cagttggccg gggtagcact ttcaccattc gcatccccct ggccacgagc
      721 atgcgcgcgc cctcactctc gccggccgcc tcgctgccca agctgcgggt gctggtcgtg
      781 gatgatgaga gcctggtcgc ggagacgttg gccggcatcc tgcgatgcct cggccattcg
      841 gtggatacag cgccgggggg ggaagaagct ctggagatct ggcgcgccga cgcctacgat
      901 ttggtaatca ccgatcacgg catgcccggc atgaccggca cggagctcgc ccagaggctg
      961 aaggagcagg cgccaaacgt gcccgtgctc ctaatcaccg ggtgggatat ccacccgggc
     1021 gtgacgacaa acacgcacga agtggtggat gccgtgcttc aaaagccgat cagccgcgag
     1081 gagctgctgc gggggataac cagcgccctt cacgctaccg ctgtgcgtgc cgcgcaagct
     1141 aacgaaacca gacgcgtgct cgtggtggat gatcaccccg ccatcaccgc ggtgattgac
     1201 ggggcgctct cctctagtgg atacaaggtc gagtgttgca cggatgccga cgcggccatc
     1261 gcccgtctgg aggaggcgct accagacctg gtgatcacgg actaccacct ggagggcact
     1321 tcagggatag atctggcacg cgtggtcaag gagaagaatc ccggcgtccc tgtcatcctc
     1381 atcaccggat ggcgccccga caagcatgag ctgagagata tcgatctgtg cctcaacaag
     1441 ccgttcaacg tccgggagct gcttgacgcc gttgccagcc tggcgaaaga tggtccacgg
     1501 gcacccaagt gagcggggca tcctcctcgc ggtctcggag gaggagccgc cccgctccat
     1561 gggtgccgac ctactgcatc gcgaacgacg ttagatagac cgccaggaat ttgggcgccg
     1621 acgggtcatc cgtctggttc aaacgcctca ggagcagttt cttcagctgc tccttcccct
     1681 tctccgaacg cagatccgcc cggcggcact gcgagagcgt gctcacaatg acatcgcgca
     1741 cgggcacctc ccattcgggc gccttttcct taccttcttt ctgctcgcca gacttcttgc
     1801 cgccggcgta ctctacgacg atcgtggtct tgacaaacgc ctgtggatcg ggctcgtcga
     1861 ggttcaccag aaactcgcct agctcatggc gggtacgctc ttcggcgctc ccgtgggccg
     1921 cgtgcgctgc cccgcctcct ccaggggtgg caacatctcg cgcccgcgcg ttcaagagcg
     1981 ccagtgtcga aacccccaag gaggctgccg agcaaaaggc aatgacgatc ataagaaatg
     2041 gcggtgcgtt ccggtatccc gccggccctg cagccgcctg cgaatcggcc atatcccttg
     2101 ttcctttccg ctctccttgc tgccacgcct cactcggcgg gcatccgtca ggcctctctt
     2161 gcccgcgtcg ccatcttctg ctccggcgag aggaaggcgc gcatcttcat ctccaccgcg
     2221 cgcgggttgt cgccgctctg tatcgccatc agcccctcga ggatcatctg atggaacatc
     2281 gcctcctcct cgccgcgcgc ccttagcttg gcagcgatcg ggagcgctac gagattggcg
     2341 aaggcaactc catagagcgt ggcgatgaac gcactggcga gggcgggccc catctgcttc
     2401 ggatctgtct gtgcccccag catatggatc accccgatga gggttccgat aacccccagc
     2461 ttgggcgaga ctgcccccat cgtggcaaag agctcctcgc cctggcggtg ccgctcatgc
     2521 atcgcgtcga tctcgatggt gagaatatcg cgcaccctct cctggggggt gccatccacg
     2581 gctagttcca cccccatgcg cagcagcgcg tgatccaccc gggccgcgtc atcctcaagt
     2641 accagcagcc cctcgcggcg cgccttttgg gcaaagccta ccatagtgtc gatcatctgc
     2701 gcccggttca gctctcggtt caagaacgcg ttccgggcga tagccggcag cttcaggatc
     2761 ttggagagcg ggaagcagat gatcgtcgcg ccgatcgtac caccgaacac cagcacggcc
     2821 gcgggcacat tgacgaacgc cgccaggttc ccgccatcca tgcagaccga gagaaccagg
     2881 cagccaaaag caagcagaag gccgatggcc gtcgctagat ccacgcgggc tctcccttca
     2941 atggccgcgc ccgcccgttc caagagcgag gcgggcgcgg tgatacgcac ggcgcgttga
     3001 ccggcatcac tgcttcagca tcagaatatc ctggagcagc tggtcggata cggtcaccga
     3061 gcgtccattc gcctggaagc cgcgctggta gacgatgagt gaagagaact cgcgcgtcag
     3121 gtcaacattc gagatctcga gcgccccgga aacgatgt
//
LOCUS       DAOSCV010000517         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19724_length_3156_cov_3.456627, whole genome shotgun sequence.
ACCESSION   DAOSCV010000517 DAOSCV010000000
VERSION     DAOSCV010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19724_length_3156_cov_3.456627"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLY56_12475"
     CDS             <1..1427
                     /locus_tag="PLY56_12475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ22339.1"
                     /translation="PSVLGPTHEEVIAFLAANEVHSYRQLPMNLYQVATKFRDEIRPR
                     FGLLRGREFIMKDAYSLDADESGLDRSYQVMAEAYHRAFARMGLPVRMVESDTGLMGG
                     KFAHEFTLLVDTDAGENTVIYCDRCDYAANMEVARSYEQSAPPAPATEAYQEMRPVAT
                     PDMKTIEQVSEFLKVPASRLVKTLLYTSGDRVIAALVRGDHELNETKLKRAVGAAVEM
                     ADAETVVRVTQAPVGFAGPVGLKVDAIIADLAVQGMANFVTGANAGDTHLLNVNHGRD
                     FAVTGWADIRNTQAGERCPLCEGTLQANPGIELGNIFKLGTKYSIALGANYTDADGNE
                     RPLVMGSYGIGITRTAQAAIEKFHDENGMIWPIPIAPFHVLVLIMNTRDEAQTRLAEE
                     VYARLQEQGVEVLLDNRDERAGAKFKDADLIGIPIQVVVGRLAGEGKVEVRRRGAPER
                     YEEAVDAALERIAAILQGADGGAG"
     gene            1435..2088
                     /locus_tag="PLY56_12480"
     CDS             1435..2088
                     /locus_tag="PLY56_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ22340.1"
                     /translation="MAIFRMAAACALGGAIGWQREAHDRPAGFRTHILVCVGACLVML
                     VSLGASSPAVTGGSAADPGRIAAQVVSGIGFLGAGTILRQGSIIRGLTTAASLWTVAA
                     IGLAAGLGGSYLLLAAAATLLVLLILTLFSHVEARMLSHRQVHGLHLAITHSEISAVL
                     GALADRGVMIGSVGSSRPDSQGVQRLHFLLRVPPHTSREELTRALITLPGVQEVEWE"
     gene            2162..2407
                     /locus_tag="PLY56_12485"
     CDS             2162..2407
                     /locus_tag="PLY56_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ22341.1"
                     /translation="MPLEDTQTSRRVQRELAKRSALDITQVNTRAIHGVVYITGRVRL
                     MRGTVNVSLEDEMQIVAQNIRRIPGIRDVVLEVSYGS"
     gene            complement(2534..>3156)
                     /locus_tag="PLY56_12490"
     CDS             complement(2534..>3156)
                     /locus_tag="PLY56_12490"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ22342.1"
                     /translation="NSFELEMPSDYTVGELTAIEQLAAHVRGIVVGLGFVEIMTNVLT
                     AKADCTERVGLPDERVVEIANVMSELYAVLRNSLVPSLLRMEGISSRATYPHRVFELG
                     EVQVYDETADLKSRTEVRLAAIIAHSTASFSEAHSYLDGLFYYLDRAYELAPVNRGTF
                     IPGRAGEILVGGKSVGFIGEVHPEVLEKWGITMPCALFEITLDALL"
BASE COUNT          565 a         1033 c         1024 g          534 t
ORIGIN      
        1 ttcccagcgt gcttggcccc acgcatgaag aggtgatcgc cttcctggcg gccaacgagg
       61 tccactctta ccgccaactc ccgatgaacc tctaccaggt ggccaccaag ttccgcgacg
      121 agatccgccc gcggtttggc ctgctgcgcg ggcgagagtt catcatgaag gatgcctaca
      181 gcctcgacgc ggatgagagt gggctcgatc gcagttacca ggtgatggcc gaggcgtatc
      241 atcgcgcctt cgcgcgcatg ggcctcccgg tgaggatggt cgagtcggac accgggctga
      301 tgggcgggaa gtttgcccac gagttcaccc tgctggtcga tacggacgca ggcgagaaca
      361 ccgtgatcta ctgtgaccgg tgtgactatg ccgccaacat ggaggtggcg cgctcctacg
      421 agcaatctgc cccgccggcg ccggccacgg aggcctacca ggagatgcgc ccggtggcca
      481 ccccggacat gaaaaccatc gagcaggttt ccgagttcct gaaggtgccc gcatcgcgcc
      541 tcgtgaagac gctcctctac acctcaggcg accgcgtgat cgccgcgctg gttcgtggcg
      601 atcacgagct gaacgagacg aagctgaaac gcgccgtcgg agcggccgtc gagatggcgg
      661 atgccgagac ggtcgttcgc gtgacgcagg cccccgttgg cttcgcgggg cccgtcggcc
      721 tgaaggtgga tgccatcatc gccgacctgg cggtgcaggg catggcgaac ttcgtcaccg
      781 gcgccaacgc tggggatacg cacctgctca acgtgaacca cggccgcgac ttcgcggtca
      841 ccggctgggc ggatatccgc aacacgcagg cgggagagag gtgcccgctc tgcgaaggaa
      901 ctctccaggc gaacccggga atcgagctgg gcaatatctt caagctgggc acgaagtact
      961 ccatcgccct cggcgccaac tacaccgatg ccgatggcaa cgagcgcccg ctcgtgatgg
     1021 gttcttacgg cattgggatc acgcgcacgg cacaggccgc catcgagaaa ttccacgatg
     1081 agaatggcat gatctggccg atcccgatcg ccccgttcca cgtcctggtg ctcatcatga
     1141 acacccgtga cgaggcgcag acgcggttgg ccgaagaagt ttacgcccgg ctgcaggagc
     1201 aaggcgtcga ggttctcctg gataaccgcg acgagcgcgc gggcgcgaag ttcaaggatg
     1261 ctgacttgat cgggattccg atccaggtcg tcgttgggcg tctcgcgggc gagggcaagg
     1321 tggaagttcg ccggcgcgga gccccggagc gttatgagga ggcggtggat gccgcgctcg
     1381 aacggatcgc cgcaatcctt cagggagcgg atggtggggc cggatgagct aggcatggcc
     1441 atctttcgca tggccgcagc ctgcgccctt ggcggggcga tcgggtggca gcgcgaggcg
     1501 cacgaccgtc ccgccggctt ccgcacccat attctggtgt gcgtgggcgc ctgccttgta
     1561 atgctcgtct cactgggcgc cagctcaccc gcagtcacag ggggctcagc cgcagacccg
     1621 gggcggatcg ccgcccaagt ggtcagcggc atcggcttcc tgggcgccgg caccatcctg
     1681 cgccagggta gcatcatccg gggattgaca accgccgcca gcctctggac cgtcgcggcc
     1741 attggtctgg cggctggcct gggcggcagc tacctgctcc tcgccgccgc agccaccctt
     1801 ctcgtactcc tgatactcac actcttcagt catgtggagg cccgcatgct ctcgcatcgc
     1861 caggtgcatg gcctccacct cgcgatcacg cactcggaaa taagcgcggt gctcggcgcc
     1921 ctcgccgatc gtggagtcat gatcgggagc gtcggctcca gccgtccaga ctcacagggc
     1981 gtgcagcgtc tccatttcct cctgcgtgtg cctccccaca cgagccggga ggaactcacc
     2041 cgcgctctga tcacgcttcc tggagtccaa gaggtcgagt gggaataacc gcccggcgct
     2101 gacgcaccgc actctccagc ttcgcacacc ggtggatgtt ccgccgagag aggagagtac
     2161 gatgccactc gaagatacgc agacgtctcg ccgcgtccag cgcgagctcg ccaagcgcag
     2221 cgctctcgac atcacgcagg tcaacacccg cgcaatacac ggcgtcgtct acatcaccgg
     2281 acgggtacgc ctcatgcgcg gcacggtcaa tgtcagcctc gaagatgaga tgcagatcgt
     2341 cgcgcagaac atcaggcgca ttccgggcat tcgcgacgtc gtcctcgagg tctcttacgg
     2401 ttcgtgacag gagctctacg cgactgtgaa cgcgcccgcg tccggcgcgg gcggatcaac
     2461 tgactgaatc gtttgaaggg cgcgcggctg aacgcccgcc cgatcaggcg acgcattcag
     2521 ccgcgcgttc tgcttagaga agcgcgtcca gggtgatttc gaagagagcg cagggcatgg
     2581 tgatgcccca cttctccaac acctcgggat ggacctcgcc gatgaagccg acggacttcc
     2641 ctcccacgag aatctcgccg gcgcgcccag gaatgaaggt gccccgattc accggggcga
     2701 gctcgtaggc ccggtccagg tagtagaaga gaccatcgag gtaggagtgc gcctcggaga
     2761 agctggccgt ggagtgggcg atgatggccg ccaggcgcac ttcggtgcgg ctcttcaggt
     2821 cggcggtctc atcatagacc tgcacctcgc ccagctcgaa cacccggtgc ggataggtgg
     2881 cccgcgagct gattccctcc atgcgcaaca atgagggcac cagcgagttg cgcagcaccg
     2941 cgtacagctc cgacatgacg ttggcgatct cgactacgcg ctcgtccggc aggccgacgc
     3001 gttccgtgca gtctgctttg gccgtcagca cgttcgtcat gatctcgacg aagcccaggc
     3061 cgacgacgat accgcgcacg tgcgccgcga gttgctcgat ggcggtcagc tcgccgacgg
     3121 tgtaatccga gggcatctcc agctcgaagc tgttgt
//
LOCUS       DAOSCV010000518         3156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19728_length_3156_cov_2.609481, whole genome shotgun sequence.
ACCESSION   DAOSCV010000518 DAOSCV010000000
VERSION     DAOSCV010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19728_length_3156_cov_2.609481"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY56_12495"
     CDS             <1..186
                     /locus_tag="PLY56_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOJ22343.1"
                     /translation="LETHDERTLLPGTGFTVEPGIYLPGEFGIRSEINVYLGHSASQV
                     TTLPMQAGIVPLLHMVE"
     gene            249..758
                     /locus_tag="PLY56_12500"
     CDS             249..758
                     /locus_tag="PLY56_12500"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOJ22344.1"
                     /translation="MPDRWWNGAGDRPAGEAETYARLEAAVRDRDMRACDGCTRCAMR
                     CVDGIDLSYPEFARLVRAAMAMPAPEQARVLGQDKVLHWGEGAVVALCPFLDRDTDRC
                     AIYPVRPLICRLFGYAPWLPCPTGKQNDSIEGAVEIMVEYASLERHPLRAWLRRYGLQ
                     LPGLSDDIE"
     gene            complement(841..2358)
                     /gene="guaB"
                     /locus_tag="PLY56_12505"
     CDS             complement(841..2358)
                     /gene="guaB"
                     /locus_tag="PLY56_12505"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ22345.1"
                     /translation="MTEEREAIADGVPAHVLFEEGLGLSYDDYILLPGYIDFHASQVS
                     LETRVTRRITIKRPIVSAPMDTVTETEMAIGLALQGGLGIIHYNNTPEEQAAMVRRVK
                     RFENGFISDPVVLGPRHTIADVDHIKQTHGFSGIPITEDGTLNSRLVGIVTNRDIDFE
                     KDRSRPLAEVMTTDLVTAPVGVTLSEANKILKESKKGKLPVVDSERRLVALVSRNDLI
                     KNKEFPEASKDPRKQLLVGAAVSTREEDRRRIDLVIEAGADVLVIDAAQGYSRFELDT
                     LRYIKETAPHVDVVAGNVVSVTQAEAMIRAGADALRIGMGPGSICITQNMMAVGRAQA
                     SAVYRCAHYARQFDVPVIADGGIRGIGHILKALAIGASTVMLGSLLAGTAESPGEYLY
                     ENGIRVKQYRGMASLEAMNAGGGKRYLNENDGVQVPQGVAGTVVDKGSLQDYVPYLHK
                     GISQGFQDMGVRSIPELHEALYSGRLRFERRTPAAQEEGSVHSLVSYREPRWGIR"
     gene            complement(2362..3063)
                     /locus_tag="PLY56_12510"
     CDS             complement(2362..3063)
                     /locus_tag="PLY56_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ22346.1"
                     /translation="MHRASLPGSSLPHAVFFDMDGLLVDSEPVHAAAYVDAFERAGLA
                     LHPDEYRAHVTLGGGSIRDLYAAKGGTPEGWPALFERKCELFAKHVREEMELLPGASE
                     LLQALADRGTPCLLATGSGRVNATVILERFGLRPYFAAILSGEDVSRAKPDPEIFLKA
                     AALIGASPAHCVSLEDSPKGVRAATGAGVPCIAVPTSWTRTGDFTGAALIVHSLQQVT
                     PLLLEEIVHNHLARS"
BASE COUNT          559 a          970 c         1060 g          567 t
ORIGIN      
        1 ctggagacgc acgacgagcg cacgcttctc cccggcaccg gattcaccgt cgaaccgggc
       61 atctatctcc cgggtgagtt tgggatccgc agcgagatca acgtctacct cgggcacagc
      121 gcgtcgcagg taaccaccct gccgatgcag gcgggtatcg ttccgctgtt gcatatggta
      181 gaatagccgg gcgcggggca ggattcgctt cctgaacggc caacacgctc tttgggagtg
      241 gctattcgat gccagacaga tggtggaatg gtgccggaga ccggccagcg ggggaagcag
      301 agacgtatgc tcgtctggaa gcagcggttc gggatcgcga tatgcgtgcc tgcgacggct
      361 gcacgcgctg cgccatgcgc tgcgtggatg gcattgacct gagctacccg gagtttgccc
      421 gcctggtacg cgctgccatg gcgatgcccg ctccggagca ggcgcgagtt ctcggccagg
      481 acaaggtgct gcattggggg gagggagcgg tggtcgcgct ctgccccttc ctggaccgcg
      541 acacggaccg gtgtgccatc tatccggtcc ggcccctcat ctgccgcctc ttcgggtatg
      601 cgccgtggct gccgtgccca accggaaagc agaacgactc gatagagggc gcggtagaga
      661 tcatggtgga gtacgcgtct ctcgagcgcc atccgctgcg cgcatggctg cgccgatacg
      721 gtctccaact cccggggcta tcggacgata tagaatagac cggcggcgcg gcggctttgg
      781 aagacgcgcg actgaacggg cggcagaggt gtgtgcagcc cattcagccg cgcgttcgag
      841 ttagcgaatg ccccagcgtg gctcgcggta ggagacgagc gagtggacgc tgccttcctc
      901 ctgggcagcg ggcgtgcgcc gttcgaaccg gaggcgtccg gagtagagcg cctcatggag
      961 ctcggggatg gagcggacgc ccatatcctg gaagccctgg ctgatcccct tatgaagata
     1021 gggcacgtag tcctgcagcg agcccttgtc cacgacggtg ccagcgaccc cctggggcac
     1081 ctgaacgccg tcattctcgt taagatagcg cttgccgccg cccgcgttca tcgcctccag
     1141 cgaggccatg ccccggtact gcttcacgcg gatgccgttc tcgtagaggt attcgcccgg
     1201 ggattccgcg gtgccggcca ggagcgaccc gagcatgacc gtgctcgcgc caatggcaag
     1261 cgccttgaga atgtggccga tgccccggat gccgccatcg gcgatgacgg gcacatcaaa
     1321 ctggcgggcg tagtgcgcgc agcggtagac ggccgaagct tgtgctcggc caaccgccat
     1381 catgttctgc gtgatgcaga tggagccagg gcccattccg atccggagcg catctgcgcc
     1441 cgcgcggatc atggcctcgg cttgcgtcac cgagaccaca ttgccggcca ccacgtccac
     1501 atggggcgcg gtctctttga tgtagcgcag ggtatcgagc tcgaaccggg agtagccctg
     1561 ggccgcgtcg atcaccagca catccgcgcc ggcttcgatc accaggtcga tgcggcgacg
     1621 atcctcttcg cgagtagaga ccgcggcgcc gaccaggagc tgcttgcgcg ggtccttcga
     1681 ggcctcggga aactccttgt tcttgatgag gtcgtttcgc gagacgaggg cgaccaggcg
     1741 gcgctcgcta tcgacaacgg gcagcttccc ctttttgctc tccttcagga tcttatttgc
     1801 ttcgctgagg gtgacgccga cgggtgccgt cacgaggtcg gtggtcatca cctcggcgag
     1861 tggccgagag cgatccttct cgaagtcaat gtctcggttg gtgacgattc cgaccagccg
     1921 ggagttgagc gtgccgtcct cggtgatcgg gatgccggag aacccgtgcg tctgcttgat
     1981 atggtcgaca tcggcgatgg tgtgcctggg tccgagcacc accgggtccg agatgaagcc
     2041 gttttcgaag cgcttcacgc gccggaccat ggcggcttgc tcctcggggg tgttgttgta
     2101 gtggatgatt ccgaggccac cctggagcgc gagcccgatc gccatctcgg tctcggtgac
     2161 ggtatccatc ggggcgctga caatggggcg cttgatggtg atacgccggg ttactcgcgt
     2221 ctcgagcgac acctggctgg cgtggaaatc aatgtacccc ggtaggagaa tgtagtcatc
     2281 ataactcagg cctagccctt cctcaaagag cacatgggcg ggcacgccat cggctattgc
     2341 ttccctttcc tcggtcatct tctagctcct cgcaaggtga ttatgaacga tctcctccag
     2401 caggagtggg gtcacttgct ggagggaatg gacgataagc gccgccccgg taaagtcccc
     2461 ggtgcgcgtc caggaggttg ggacggcgat gcaagggacg ccggcgcccg tggcggcccg
     2521 cacgcccttg ggggagtcct caagtgagac acagtgggcg ggagacgcgc caatcagggc
     2581 cgcggctttc agaaatatct cgggatcggg cttggctcgg gagacatcct caccactcaa
     2641 aatggccgcg aagtaggggc gcagcccgaa gcgctccagg atgaccgtgg cgttgacacg
     2701 tcctgaaccc gtcgccagaa ggcaaggagt gccccgatca gccagggcct gcaacagctc
     2761 cgaggcaccc gggagcagct ccatctcctc gcgcacatgt ttcgcgaaca gctcgcactt
     2821 gcgctcaaac agcgccggcc agccctccgg ggtgccgccc ttggcggcgt agaggtcacg
     2881 gatggagcct ccacccaggg tgacatgcgc gcgatactca tccgggtgca gcgccaaacc
     2941 ggctcgctcg aaggcgtcga cgtaggccgc ggcgtgcact ggctcagagt caaccaggag
     3001 accgtccatg tcgaagaaaa cggcgtgagg gagggaagag cctggcaaag acgcacgatg
     3061 catgcccaca gcgccttcct ccggaagggt gtgaacgagc cgcggtaagt gtcccatagg
     3121 ttattatagc atggatcaca ggcccggagc aagtgg
//
LOCUS       DAOSCV010000519         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19810_length_3146_cov_4.064057, whole genome shotgun sequence.
ACCESSION   DAOSCV010000519 DAOSCV010000000
VERSION     DAOSCV010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19810_length_3146_cov_4.064057"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1278..2222
                     /locus_tag="PLY56_12515"
     CDS             1278..2222
                     /locus_tag="PLY56_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ22347.1"
                     /translation="MKVLVTGGAGFIGSHVVDRLIADGHQVVVVDDLSNGKRENLNPA
                     ATFYQMDIQDPALADLFEKEKPEIVNHHAAQMDVRRAVEDPVFDARVNILGFLNVLEN
                     CVRHQVRKVIFISSGGAVYGEPEQLPAEETHPIRPITPYGVTKYTGEKYLYFYRAVHK
                     LDYTVLRYANVYGPRQDPHGEAGVVAIFIGLLARGETPTVFRYPDGEMLRDYVFVRDV
                     VEANIAAMSRGSGQEFNIGTGRGTSVSDLLAAVQRVMGTDIEPRYGAARPGEIRQTYL
                     AIERAARLLDWRPKVSLEQGLYETAEWFRQHAQAKKPA"
     gene            2490..>3146
                     /locus_tag="PLY56_12520"
     CDS             2490..>3146
                     /locus_tag="PLY56_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ22348.1"
                     /translation="MADLRAQYLGMKAEIDRAVLEVLESAQFILGDRVARLETRIASY
                     CGAKHAVGVASGTDALLLPLVAMGIGPGDEVITSPFTFVATVEVICLAGATPVFADIQ
                     PDTFTLDPASVASKITPRTRAIISVHLYGQLASVTELRALAERYGLKIVEDAAQAIGA
                     AQQGVRAGAWGDAAGFSFFPTKNLGAAGDGGMVTTDDEELAAKMRELRFHGSGGTYYY
                     R"
BASE COUNT          547 a          943 c         1070 g          586 t
ORIGIN      
        1 ttagcaagac cctgtcgacc tgggtcaggt tggttgcacg cttgatgagg ctgcgaacgg
       61 ccctgatctg cctttcagga tcgagaaact gcgcatcgta atcggtcaac tcatgcctcc
      121 cggctcgcta gataccgcac ggagctggac ataagcctat gaagcctgtg ggtacgaaag
      181 gtatcatgcc ccgcacggat ggggagtgtg ccggatatcc cggggtgcga gagttttccg
      241 ggcaccccct tggaactcga tcaagggaac acaccttgcg atcgatgcct caccaggtat
      301 tatactacca tgcggaccac ttggcaagat cacggcgagc tcggctggga ttccgagata
      361 aggagagtgc cgcgcacgcg cggagctcga agctccgcgc tgcttgtgag ccaagccctg
      421 ccgggctggg ttggggatac tcccagacgt tgcaaaacgg cggtggctgg gaggggaagc
      481 cctgaaaggg ctttgccgga aagcagcgcg gtgcttttag cgccgcgcgc cgggttgcgc
      541 gctgcatccg aggcttcgga ttccttatct tggaatctca gcgatcacgg tttcctgcgt
      601 cgtgtcggga gcgacgcgaa aaccctctcc aaccggcttc gccagtcgca cggccgactc
      661 ggatcccagg agttcggaaa cccccggcgg ggatgttgga ccccttcggt gccgtccgca
      721 atcgcctcgc ggtagctcct ccccccggga tacagcgccg gccagagggg tggggaaggg
      781 ggcttccgat ccgcgtccgt tcccaacatc tgtggtatcg tttcgggcag gggcgggagg
      841 gcagacaagc cgctctgcgc gaggagcggg cgcttgactt cgcatgcagg ctggtggtac
      901 aatacgcatg cttcacatgg gggaatctct gccgcgtgga gcagtccagg cgctggacca
      961 tcccgcggga tgcgcgccag gggctccgcg ccgccgcggg atgctgcccc gccctcgctt
     1021 gtcgccggca aggcattctg gcgtccgcgt ctccggctat atcggcaccg gtgttcatgc
     1081 acccactcgg tcgcgccggc gcccatgccc gccctggaag atgggcgatg caggtgggac
     1141 gactgaatgg acggtttgcg gaatagacga cggaacgcag cactggcgtt gtgcccattc
     1201 agttacgtct tctgcttcgt caggcgaccg cgcccctcca cgggtccgcg gcgacgtgtg
     1261 ttgaaggagg aaaatctgtg aaggtcttgg tgaccggtgg cgctggcttc atcgggtcgc
     1321 acgtcgtgga tcgtctcatc gccgatggac accaggtggt ggtggtagac gatctttcga
     1381 acggcaagcg cgagaacctg aatcccgctg cgactttcta ccagatggat attcaggatc
     1441 ctgccctggc ggacctcttt gagaaggaga agccggagat cgtgaatcat cacgcggcgc
     1501 agatggatgt acgccgggcg gtggaggatc ctgtgttcga tgcccgggtc aacattctcg
     1561 ggtttctgaa cgtgctcgaa aactgcgtgc gtcaccaggt gcgcaaggtg atctttatct
     1621 cctccggagg ggcggtgtat ggcgagccgg agcagcttcc cgccgaagag acgcacccga
     1681 tccggcccat cacaccctat ggtgtaacca agtacacggg tgagaaatac ctctatttct
     1741 atcgggccgt ccacaagctc gactacacgg tgctccgcta tgccaacgtc tacggacccc
     1801 ggcaggaccc gcacggcgag gccggcgtgg tggccatctt catcggtctg ctggctcggg
     1861 gcgagacgcc gacggtcttc cgctatcccg acggcgagat gctgcgcgac tacgtctttg
     1921 tgcgcgacgt ggtggaggcc aacatcgcgg cgatgtcgcg aggctccggg caggagttca
     1981 acattggcac cggtcgtggc acctcggtga gcgacctgct cgcggcggtg cagcgcgtga
     2041 tgggcaccga catcgagccg cgatatggcg cggcacggcc gggtgagatc cgccagacct
     2101 acctcgccat cgagcgcgcc gcgcgcctgc ttgactggcg cccgaaggtg agcctggaac
     2161 agggcctgta cgagaccgcc gagtggttcc gacagcacgc gcaggccaag aagcccgcgt
     2221 aggcggccct tcgcgagagc gcgagggggc agggctctgg gcgggtgaac cgcctctgat
     2281 gccgcggccc ggctcgtggc ctcgctcgct tgcttgaaaa agcatctacg catctggtat
     2341 aatcggtcca tgcgcgtgtg cgcgcttgtt atcgcccatc ggcgggtgag agatgccgtt
     2401 gatgccttgg agacgacgct cctgagacag acgcatcttc gcacggatgg gtgccgggaa
     2461 aggtggactc tggggtaatg caagtaccca tggccgacct tcgtgcgcag tatctgggca
     2521 tgaaggcgga gatcgaccga gcggtgctcg aagtacttga gtcggcgcag ttcatcctgg
     2581 gcgaccgggt ggcgcggttg gagactcgta tcgccagcta ttgtggagca aaacacgcgg
     2641 tcggagtcgc ctccggcacg gatgcgctgc tccttcccct ggtcgcgatg gggatcggcc
     2701 ccggcgatga ggtgatcacg tcgcccttca ccttcgtggc aacggtggag gtgatctgtc
     2761 tggcaggcgc gacgccggtg tttgccgaca tccagccgga taccttcacg ctggatcccg
     2821 cttccgtagc ctcgaagatc acaccccgaa cgcgcgccat catttcggtg catctctacg
     2881 ggcagctcgc gtcggtgacg gaactgcgcg cgcttgccga gcgctatggc ctgaagatcg
     2941 ttgaggacgc ggcgcaggcg attggcgcgg cgcagcaggg cgtgcgagct ggcgcctggg
     3001 gcgatgccgc cggcttcagc tttttcccga cgaagaacct gggtgcggcg ggcgacgggg
     3061 gaatggtcac caccgacgat gaagagctgg cggcgaagat gcgagagctt cgcttccatg
     3121 gcagtggtgg gacctactac tacagg
//
LOCUS       DAOSCV010000520         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19842_length_3142_cov_3.733722, whole genome shotgun sequence.
ACCESSION   DAOSCV010000520 DAOSCV010000000
VERSION     DAOSCV010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19842_length_3142_cov_3.733722"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..208
                     /locus_tag="PLY56_12525"
     tRNA            134..208
                     /locus_tag="PLY56_12525"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:166..168,aa:Ile,seq:cat)
     gene            389..1174
                     /locus_tag="PLY56_12530"
     CDS             389..1174
                     /locus_tag="PLY56_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22349.1"
                     /translation="MAGWIWRYDAGGQFFGSFVISLAGISITVLTLVLALPKEHRHTG
                     HFRLLYTTLVVATLACIQGAHLMGDAASWESRFSGAAPYEGSIQAFMTAAPTGYVATV
                     LVMFALVLLPKVYDPTFPRIIRYILLATLAAEVVGSWLSMIPQVWSGAGVSHNKPAVI
                     VAVIGGIGLGVYEARAHGSDFADYLPLIICWVIVCLCGAVYGIVCPKFAEGHWWLITW
                     LYALGATWPSSALIGCACRLTAHPENMPQKSETDERVPSLTWE"
     gene            1468..>3142
                     /locus_tag="PLY56_12535"
     CDS             1468..>3142
                     /locus_tag="PLY56_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4965 domain-containing protein"
                     /protein_id="HOJ22350.1"
                     /translation="MQLFETVVSNLGSRFTLNLLPHRRQLLLSPLGYYFHVPVDLAVG
                     IQIGDDYRILPFSDRYKCFDSVEQELLPSGVVFHCKEPELGVMVDIAFRSAFYPHDVI
                     LSTAPFCYVSVTVSRLAGRQNKPRPIEGKVFIAARPGPEFSARRIDGGFVFSGKFSPN
                     GTRRDLHLPEGFSDGVPAEFGIAAVDPDPELGDRRISQRFLLDDQEFVEEEFTLAGYC
                     GAECLEAGGERYRFKYSGFFASVDDVFAYASSEKNHIDEKMALFDGVIRDSTVPRDLQ
                     QLIAFSLQTYLANSFWTTRHGQDWFSVWEGNCLYHSTVDVEYNVGLFYLALWPELLEM
                     TLEEWTKHEQRGLYGSYLSHDMGAGMMANGQSYPHAMEVEENTNFLLMAFACWRATGR
                     EHFIATHYPLIRRLAKYVIDADTTGNGFPDLGVANTFDDAAPAVQFSRKQVYLAVKAL
                     AAFEVTALMAESNGDEEFAGICRDRAALIQQTLDTEAWQGDHYAVCLEKRMDEITDPW
                     SGKPAGTGELPGWDAYSIHTANGLLYPLLSGYRPNLDYARLARDVAHATR"
BASE COUNT          518 a          997 c          962 g          665 t
ORIGIN      
        1 gcggtgattt atcgccgcgc gccgggttgc accctgcctc cgaggcttcg gattcattat
       61 ctcggaatct cagatctgca ccttgacagg tgcatgccgc gcctgttatg ataccgctgg
      121 caaaatgcct ctcgggccga tagctcaacg gcagagcagg ggactcataa tcccttggtt
      181 gcaggttcga atcctgctcg gcccaccaag atgtacccct tcaatgacct ggatgcctcg
      241 gcatgttgcg tccctgactg ttcggtttcc atcctcgagt agcgtgctgc aaacagacgg
      301 ccggcggcgt ctgtgcgatg gaccgcttgc agccagcact ccgcgtctgc aacgccgcgc
      361 gtagcgggcg tacgcgtagg agggtgacat ggcaggctgg atatggcgct atgacgctgg
      421 cgggcagttc ttcggtagct tcgtgatctc gctggcgggc atcagcatca ccgtcctcac
      481 cttggtactc gccctaccga aggagcaccg gcacaccggg cacttccggc tgctttacac
      541 gacgctggtg gtcgccaccc tcgcttgcat acagggtgcg cacctgatgg gcgatgcggc
      601 cagctgggag tcccgtttca gcggcgcggc cccctacgaa ggcagcatac aggcgttcat
      661 gactgcggct cctacgggct atgtcgcgac ggtattagtg atgttcgccc tggtgctcct
      721 gccaaaggtt tacgatccca ccttcccacg gatcatccgc tacattctcc tcgccacgct
      781 cgcggcggag gtggtcgggt cgtggctctc catgataccg caggtgtggt cgggagcggg
      841 tgtcagtcat aacaagccgg ccgtcattgt cgccgtgatc gggggtattg gtttgggcgt
      901 gtatgaagcg cgcgcgcatg ggagcgattt cgcggactac ctgcccctga tcatctgctg
      961 ggttattgtc tgcctgtgcg gggctgtcta tggcattgtg tgcccgaagt tcgcggaagg
     1021 gcattggtgg ctgattacct ggctctacgc gctgggcgcg acatggccaa gcagcgctct
     1081 tatcgggtgc gcatgccggt tgaccgccca tccagagaac atgcctcaga agagtgaaac
     1141 cgacgagcga gttccgtcgc taacctggga gtgatgaggt ccattgcggc cgttatgcag
     1201 cgtgcctttt gcagttcctc tcccgtaacg ttgggcgatc atcttctgcg cgtgctaacg
     1261 atgaaatcgg ggtgggatcc atgtaatcct gccgggcgta tcgcatccac cctcacggag
     1321 tatccactgt gtgaacgcgg cgcaacgtgg gacgtctagt ctgcccctcg tccctcgagc
     1381 cccggagtgt gagaggattc gctgtgcctt ggcgagaaca cccttataag ccgtgcgggt
     1441 ctgcacggcc gggaaaggat agcgcccatg cagctctttg agacggtggt gtcgaacctc
     1501 ggctcgcgct tcacgctcaa cctgctcccg caccgccgcc agcttctctt gtcgccgctc
     1561 ggatactact tccatgtgcc ggtcgacctc gctgtcggca tccagatcgg cgatgactat
     1621 cgcattcttc ctttctcgga ccgctacaag tgcttcgact ccgtggaaca ggagctgctc
     1681 ccgagcggtg tcgtcttcca ctgcaaggag ccggagcttg gggtgatggt ggatatcgcc
     1741 ttccgctccg ccttctatcc ccacgatgtc attctgtcga cggcaccgtt ctgttacgtc
     1801 agcgtcactg tctcgcgcct tgcgggccgc cagaacaagc cgcggcccat cgaggggaag
     1861 gtctttatcg cagcgcgccc cgggcccgag ttctccgcgc ggcgaatcga cgggggcttc
     1921 gtcttctccg gcaagttctc ccccaacggt acgcgacgcg acctccacct gcccgagggc
     1981 ttctccgatg gcgttcccgc tgaatttggg atcgccgcgg ttgaccccga tccggagctg
     2041 ggcgaccggc ggatctctca gcgtttcctc ctcgatgatc aggagttcgt ggaagaagag
     2101 ttcaccctcg ctggctactg tggcgcggag tgccttgagg cgggcggcga gcgctaccgc
     2161 ttcaaatact ccgggttctt cgcgtcggtg gatgacgtct ttgcctacgc ctcgtccgag
     2221 aagaaccaca ttgacgagaa gatggcgctc tttgacgggg tgatccgtga tagcaccgtg
     2281 cctcgcgatc tgcagcagct tatcgccttc tcgctgcaga cgtacctggc caactccttc
     2341 tggacgacgc ggcacgggca ggactggttt tccgtttggg aaggcaactg cctctatcac
     2401 tccacggtgg atgtggagta caacgtcggg ctgttctacc tggccctctg gccggagctg
     2461 cttgagatga ccctggaaga gtggacgaag catgagcaac ggggtctcta tggcagctac
     2521 ctgtcgcacg atatgggcgc cgggatgatg gccaacggcc aatcctaccc gcacgcgatg
     2581 gaggtggagg agaacaccaa cttcctgctg atggcgtttg cctgctggcg ggccacgggc
     2641 cgcgagcact tcattgccac ccactacccg ctgattcgcc ggctcgcgaa gtatgtcatc
     2701 gatgccgata cgaccggcaa cggcttcccg gatctcggtg tcgccaacac cttcgatgat
     2761 gccgcgccag ccgtccagtt ctcgcgcaag caggtttacc tcgcggtcaa ggcgctggcc
     2821 gctttcgaag tcaccgcgct catggccgag tccaacggcg atgaggagtt cgccggtatc
     2881 tgtcgcgacc gcgcggcgct catccagcag actctcgaca cggaggcgtg gcagggcgac
     2941 cactacgccg tgtgcctgga gaagcgcatg gatgagatta ccgacccctg gagcggaaag
     3001 ccggccggca ccggcgagct tcccgggtgg gatgcctact ccatccatac ggccaatggc
     3061 ctcctctatc cgcttctctc cggctatcgc cccaacctgg actacgcgcg cctcgcccgc
     3121 gacgtcgccc acgccacgcg cg
//
LOCUS       DAOSCV010000521         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19844_length_3142_cov_3.201814, whole genome shotgun sequence.
ACCESSION   DAOSCV010000521 DAOSCV010000000
VERSION     DAOSCV010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19844_length_3142_cov_3.201814"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLY56_12540"
     CDS             complement(<1..586)
                     /locus_tag="PLY56_12540"
                     /inference="COORDINATES: protein motif:HMM:NF027862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein T N-terminal
                     domain-containing protein"
                     /protein_id="HOJ22351.1"
                     /translation="MRASRLCVLVVVALAWGALGGASGAPQSKDEQPEPLRIVEAEGF
                     APIVDGNISNARQAAIDRALREAVLRTVGVYVTGSVRVHNHIVQEDRVSTRAAGLAIL
                     EEVLEEGRADHVYRVRARVAVRLRPLLRELSSSPAVRRWRVGIVLASSTYYGYVPTAA
                     SDAAALLSRHLESLGIDADDQGAEVSGEEARQLQS"
     gene            728..982
                     /locus_tag="PLY56_12545"
     CDS             728..982
                     /locus_tag="PLY56_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22352.1"
                     /translation="MELRCQLRFVDDADGKRAFLEARDARGCVRVTIEATGGDEGEAL
                     SALAERTRELYGAVCGIVDTVEDVVRRYYDSERVETIPTE"
     gene            complement(1139..1411)
                     /locus_tag="PLY56_12550"
     CDS             complement(1139..1411)
                     /locus_tag="PLY56_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22353.1"
                     /translation="MDFDPDAPELDLLQGVIDPDSVQIEVAPDDEADAATGAAVRVLR
                     QIEEAYATGNPQLRIPCPFCGRPLRYQKNEHGCLAECPGNDFVCMG"
     gene            complement(1502..2086)
                     /locus_tag="PLY56_12555"
     CDS             complement(1502..2086)
                     /locus_tag="PLY56_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22354.1"
                     /translation="MLVAAGVGSVAYLYTMVTYTPPPPPPPQVSPQQAERSVRQLQEQ
                     VVAIRRHAQTRPPAPYRLQIRSDDINAYLSAQAPPAHGLPAGIESVRDVRVAFEGGRI
                     VVTGTVTAWGRQVHTTVSGRVVPDGSGSLAFLPDEMMIGKMPMPAQMRSEAARRIQQE
                     LQKILAETGVSVHSIEATSGSMVIEGMTTGQRSR"
     gene            complement(2146..>3142)
                     /locus_tag="PLY56_12560"
     CDS             complement(2146..>3142)
                     /locus_tag="PLY56_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOJ22355.1"
                     /translation="VYYTTEEGTRRISTETGAGQWGSALSMACNFFGLECKVYMVKVS
                     YHQKPYRRSLMQMWGANVVASPSEDTNAGREILARDPDSPGSLGMAISEAVEDAVGRE
                     DTKYSLGSVLNHVLLHQTVIGLEAKKQFEMVDDYPDVIIGCAGGGSNFAGLAFPFVPD
                     KLSGKKIRIIAVEPTACPTLTRGIRHYDFGDVARMTPLMMMYTLGHDFVPTAIHAGGL
                     RYHGMAPLVSELYHQGLIEAKAYPQNPVFAAATLFAKTEGIVPAPESSHAIRAAIDEA
                     ERCKREGRKETIVFNLSGHGHFDLSAYEAYMAGKLEDYDYPEEKVQDALARLENL"
BASE COUNT          521 a         1030 c         1023 g          568 t
ORIGIN      
        1 cagactgcaa ctgccgcgcc tcttccccgg agacctccgc tccctggtca tccgcatcga
       61 tcccgaggct ttccaggtgt cgggagagga gagccgccgc atccgatgcc gcagtgggca
      121 catagccata gtaggtgctg gatgccagca cgattcccac tcgccagcgc cgcacggcag
      181 gggatgacga gagctccctc agcaatgggc gcaggcgcac ggcgacccta gcgcgcaccc
      241 gatagacgtg atccgcgcgc ccctcctcca ggacctcctc cagaatggca aggcccgcgg
      301 ctcgagtgga gacgcgatcc tcctggacga tgtggttgtg aacccggacg ctgccggtga
      361 cgtacacccc cacggtgcgc agcacggcct cgcgcagggc gcggtcgatg gcggcctgcc
      421 gcgcgttact gatattccca tcaacgattg gagcgaatcc ctcggcctcg acaattcgca
      481 gaggctctgg ctgctcgtcc ttggattggg gcgcgccgct ggcgcctccc agggcgcccc
      541 atgccagcgc gaccacgacc agcacgcaga gccgtgatgc tctcatcgcc acctcccact
      601 ggcgacccgc aatcaccgtt cgcattcgtg gcatgcaagg atgcagccaa tggagtaggg
      661 gccttcacgg tgcaatcctc tttacgctgc gggaggtgtc gcgcgccacg cggcgaatct
      721 tctccacgtg gaactgcgtt gtcagctgag gtttgtggat gacgcggatg gcaagcgcgc
      781 ttttctcgag gcgcgtgatg cccgcgggtg cgttcgggtc accatcgagg cgaccggcgg
      841 ggatgagggg gaggcactct ccgcgctcgc ggagcgcacg cgagagctgt atggcgccgt
      901 ctgcggcatc gtggacacgg tcgaggatgt tgtgcgccgc tactatgaca gcgagcgcgt
      961 ggagaccatt ccgacagagt gatggccaaa gccatctctt gggcgaaggg gcgttccgtt
     1021 ccgggacttc ccggtgggag agaccgtcag gagccggggg agtatccgtt cggtgggttg
     1081 cgattccttg tgccgaaacc ggtgtggcag ttcgatttgg ccggctactg gtgccgcttc
     1141 aacccatgca gacgaaatcg ttgcccgggc attcggccag gcagccgtgc tcattcttct
     1201 ggtagcgtag cgggcgtccg cagaaggggc agggaatacg caactgtgga ttcccggtag
     1261 cgtaggcctc ctcgatctgg cgcaggacgc gaaccgctgc gccggtggcg gcatccgctt
     1321 cgtcgtcggg ggccacctca atctgcaccg agtctggatc gatcacgcct tgcaagagat
     1381 ccagctccgg ggcatccgga tcgaaatcca tcctcttctc ctctatgttc cctcggatcg
     1441 cccgtggtca agagaggggc gcggcgcggg atgcctctcc agcgccacgc caccgatcat
     1501 atcagcgaga gcgctgacca gtggtcatgc cctcgatcac catgctgccg ctcgttgcct
     1561 cgatggagtg gacggaaacg cccgtctctg cgaggatctt ctgcagctct tgctggatgc
     1621 gccgggcggc ctcgctgcgc atctgcgcgg gcatgggcat cttcccgatc atcatttcat
     1681 ccggaagaaa ggcaaggctc cccgacccat ccggaacgac acggcctgac acggtggtgt
     1741 gcacctgccg gccccaggcg gtcaccgtgc ccgtcacgac gatgcggccg ccctcaaacg
     1801 cgacgcgcac gtcgcggacg ctttcgatgc cggccggcaa cccgtgggcg ggcggcgcct
     1861 gcgcgctcag atacgcattg atgtcgtcgc tgcgaatctg caagcgatag ggcgccggag
     1921 ggcgcgtctg cgcgtgccgc cggatcgcga ccacctgctc ctgtagctgg cgcaccgacc
     1981 gctccgcctg ctgcggggaa acctggggtg gcggtggcgg cgggggcgtg taggtcacca
     2041 tcgtgtagag gtacgctacg ctcccgacgc ctgccgccac cagcaacagg gcagcccagc
     2101 ccaggcggcg tatccaaagc cgtcgtctat ccgatccggg cgacctcaaa ggttctccaa
     2161 tcgcgccaga gcgtcttgga ccttctcctc gggatagtcg tagtcttcga gcttgccggc
     2221 catatacgct tcataggccg atagatcaaa gtgaccgtgg ccactcaggt tgaagacaat
     2281 cgtctccttc cggccctcgc gcttgcaccg ctcggcctca tcgatcgcgg cgcgaatggc
     2341 gtgcgacgac tcgggggctg gtacaatgcc ttccgtcttg gcgaagagcg tggctgctgc
     2401 gaagacgggg ttctgggggt aggccttggc ctcaatcagc ccctggtggt acaactcgct
     2461 gaccagcggg gccatgccgt gatagcggag gccgccggca tggatcgccg tgggcacaaa
     2521 gtcgtggccg agcgtgtaca tcatcatcaa cggcgtcatg cgcgccacgt caccgaagtc
     2581 atagtgtcgg atgccgcgag tgagcgtcgg gcacgcggtc ggctccaccg cgatgatgcg
     2641 gatcttcttg ccgctcagct tatccggcac gaaggggaaa gcgagcccgg cgaagttgct
     2701 gccgccaccc gcgcagccga tgatgacgtc aggatagtcg tccaccatct cgaactgctt
     2761 cttggcctcc aggccgatca cggtctggtg cagcaggacg tggttcaaaa cgctcccgag
     2821 ggaatacttg gtatcctcgc gcccgacggc atcctcgacg gcctcgctaa tcgccatgcc
     2881 caggcttccg ggggagtcgg gatcgcgtgc gaggatctct cgcccggcgt tcgtgtcctc
     2941 gctcgggctg gcgaccacgt tggcgcccca catctgcatc agcgagcggc ggtaaggctt
     3001 ctggtgatag ctcaccttca ccatgtagac cttgcactcc aacccgaaga agttgcaggc
     3061 catcgagagg gcgctgcccc actgcccggc gccggtttcg gtggagatgc gccgcgtgcc
     3121 ttcctccgtg gtgtagtaga cc
//
LOCUS       DAOSCV010000522         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19847_length_3142_cov_2.966958, whole genome shotgun sequence.
ACCESSION   DAOSCV010000522 DAOSCV010000000
VERSION     DAOSCV010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19847_length_3142_cov_2.966958"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PLY56_12565"
     CDS             complement(<1..724)
                     /locus_tag="PLY56_12565"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ22356.1"
                     /translation="MGETIREPAREIPVTHEADVVVAGGGTAGVACAVCAARLGLSVI
                     MVENTAQPGGMVTHVTQWTNDFDNKGGFAREFYEHLVENGILVRPYYNPFRVVPYLDS
                     LLEEAGVLPLYLCRVAAPIVEDGELQGVIVESKQGRHAIRARVVVDATGDGDVAAFAG
                     ASFEFGREGDGAIQSISLTHSMQGFPRDRVHLRDELLPMLRQVDPECQLVYDNGYIRR
                     QVDTRAGLLFGTPHVFGYNPLDA"
     gene            complement(778..1722)
                     /locus_tag="PLY56_12570"
     CDS             complement(778..1722)
                     /locus_tag="PLY56_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose oxidase"
                     /protein_id="HOJ22357.1"
                     /translation="MHEVKQAPDWECATRDAGWRARDSMGEFVYDGHLWILGGWFAAQ
                     EPNPLDVWKSPDGKHWTCVQEHAPWVQSDLPVSLVFDGRMWIMGGRKLPGTECSNKVW
                     SSTDGVQWTLEGEAGWCPRVAAGFAVFKDRMWVMSGTESFYDHSDRMVKNDVWSSPDG
                     KTWELVTPEAPWSKRAHGQVVVFKEKLWILGGGRWHPEHAVTSDVWCSEDGAHWTQVT
                     EAAPWAPRIWFSALVYRDRLWVLGGWSKEHGNFGDVWCSENGRDWIEVRSNVIWTPRH
                     EHAAYVFQDRIWVAGGYAERLSNEVWSLEILPEWFEAR"
     gene            1995..2447
                     /locus_tag="PLY56_12575"
     CDS             1995..2447
                     /locus_tag="PLY56_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ22358.1"
                     /translation="MTAREIMAAPVITAGPNATLTEISELMLAHRISGIPIVDSEGRL
                     MGIVTESDMLEEDKRRQLLPRVTIFGMVPAHQGPVEDALLKARTFRASEVMTKKVFTV
                     EPDTPVTEVAGLLVRERINRVPVVEEGKVIGIVSRADLIRALHQSGSL"
     gene            complement(2631..2930)
                     /locus_tag="PLY56_12580"
     CDS             complement(2631..2930)
                     /locus_tag="PLY56_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22359.1"
                     /translation="MRHTRLTAALMGIGAAVTLLLPATGASADPGRGHPVKPRVRQEV
                     RHGHRLGHHRKPHKPQVRRDARRDAYRKGYKDGLRDARRFAHRRDGRRIYRGPRS"
BASE COUNT          533 a         1119 c          979 g          511 t
ORIGIN      
        1 ccgcgtctag cgggttgtag ccaaacacgt ggggagtgcc gaacaggaga ccggcacgcg
       61 tgtccacctg ccgccggatg tagccattgt cgtagacaag ctggcactcc gggtctacct
      121 ggcggagcat cggcaggagc tcgtcacgga ggtggacccg gtcacgcggg aagccctgca
      181 tcgagtgcgt gagcgagatc gactggatcg ccccatcgcc ctcgcgcccg aactcgaaac
      241 tcgcgccggc gaaggcggcc acatcgccat cgcctgtggc atccaccacg acgcgggcgc
      301 gaatcgcgtg ccggccctgc ttcgattcga cgatcacccc ctgcaactct ccatcttcca
      361 cgatgggcgc cgccacccgg cagaggtaga gcgggagcac gccggcttcc tcaagcagcg
      421 agtcgagata gggcaccacc cggaaagggt tgtagtaggg ccgcaccaaa atcccattct
      481 cgactaggtg ctcgtagaac tcgcgcgcga acccgccctt gttgtcgaag tcgttcgtcc
      541 actgcgtgac atgcgtcacc atgccgcccg gctgcgccgt gttctcgacc atgatgacgg
      601 agaggcccag gcgcgcggcg cacacggcgc acgccacgcc cgccgtgccg cctcccgcga
      661 ccaccacgtc ggcctcgtgc gtcaccggta tctctcgcgc aggctcgcga atcgtctctc
      721 ccatcgctca ctcctcttca cgccagggtg cccccctacc aggcacccgc cgcaacccta
      781 ccgagcttca aaccactccg gcaggatctc cagcgaccag acctcgttgc tcaaccgctc
      841 ggcgtagccg ccggccaccc agatccggtc ctggaagacg taggcggcgt gctcgtgccg
      901 tggcgtccag atcacattcg agcgaacctc gatccagtcg cgcccgttct cagagcacca
      961 cacgtcgccg aagttcccat gctccttcga ccacccgccc agaacccaga gccggtctcg
     1021 gtagacaagc gccgaaaacc agatgcgcgg cgcccagggg gctgcttccg tcacctgagt
     1081 ccagtgggcg ccatcctcgg agcaccacac gtcgctcgtc acggcgtgct ccggatgcca
     1141 ccgcccgcca cccaggatcc acagcttctc cttgaaaacc accacctggc catgcgcccg
     1201 ctttgaccag ggcgcctccg gcgtcaccag ctcccacgtc ttgccatccg gcgaggacca
     1261 gacatcgttc ttcaccatcc ggtcgctgtg gtcgtagaag ctctcggtgc cgctcatcac
     1321 ccacatgcgg tccttgaaga cggcgaaccc cgcggccacg cgcgggcacc agccggcctc
     1381 tccctccagc gtccactgga cgccatcggt cgacgaccac accttgttgc tgcactcggt
     1441 tcccggcagc ttgcgcccgc ccatgatcca catccgcccg tcaaacacga gcgacaccgg
     1501 caggtcgctc tgcacccacg gggcgtgctc ctgaacgcac gtccagtgct tcccatcggg
     1561 cgacttccag acgtcgagcg ggttcggctc ctgcgcggcg aaccagccgc ccaggatcca
     1621 gaggtggccg tcgtaaacaa actcgcccat cgagtcgcgc gctcgccagc ccgcgtcccg
     1681 ggtcgcgcat tcccagtccg gcgcttgctt cacttcgtgc atggcatccc ccttcgccgc
     1741 gggagagttc gacgcccccg ggcgcgctcc tcttgccctg gtagctcggg acctatggaa
     1801 tgcctgctct cgtaccctgg gggcaatcgg tagtcacagg ggcgaagcag cacaggcgcg
     1861 tggactccaa acccgccggg aggagccatc tcctctcatt ccgaacaaaa acaacgggag
     1921 tgcagacccg gatgctgcgc gacgtgacgg cctccgcccg gtggagccat cactcaaggg
     1981 aagggagaga cacgatgacg gcacgcgaga tcatggcggc tccggtcatt acggccggcc
     2041 cgaacgcaac cctgacggag atttccgagt tgatgctggc ccaccgcatc agtgggattc
     2101 cgatcgtcga ttcggaaggc cggctgatgg ggatcgttac cgagagtgac atgctcgagg
     2161 aggacaagcg gaggcagctg ttgccgcgcg ttacgatctt cgggatggta ccggctcacc
     2221 aaggccccgt agaggacgcg ctgctgaagg cgcgcacttt ccgcgccagc gaagtgatga
     2281 cgaaaaaggt cttcaccgtc gagcccgaca cgcccgtcac cgaggtagcc ggcctcctgg
     2341 tgcgcgagcg gatcaaccgc gtgcccgtcg tcgaggaggg caaggtgatc ggcattgtca
     2401 gccgggccga tctgatccgc gcgcttcatc agtccggctc gctgtagtgg ctgggcgcgc
     2461 gcgctcgcgg gccggtacgc agcgcggccg gggcgcgcgc agggcacacc ccggccagtg
     2521 cgatttcctc accgaaaggt cttctcttgt tcgacagggc aaccggcggc ccccatgctc
     2581 aggccgagtc gccggtcacc cggatgaagc cgctggcttc cctcgcctgt tcagctgcgg
     2641 gggccgcggt agatgcggcg gccatcacgg cggtgtgcga agcggcgcgc atcccgcagg
     2701 ccatccttgt agcccttgcg gtaggcgtca cgtcgggcat cgcgacgaac ctggggcttg
     2761 tggggcttgc ggtggtgccc aagccggtgg ccgtggcgga cctcctggcg aaccctcggc
     2821 ttcaccgggt gaccccgccc cggatcggct gaggcgccgg ttgctggaag cagaagggtg
     2881 acggcggcgc cgatccccat gagcgcagcg gtaagcctgg tatgtcgcat cgaacgtctc
     2941 ctttcgcgca tgtcgtcctt attgggctca cctcgagcag caggaaatag gatggaatcc
     3001 tgaagctcga agaggatact gccccatctg ggcttcgctt agaatgctgc tccgaggggc
     3061 gttctgcgat gatttgcggt gacgacggtc gaggcgcatc gggcgcgccc cgaccggggc
     3121 ccctcgggtg ccggagccct at
//
LOCUS       DAOSCV010000523         3136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19895_length_3136_cov_2.587147, whole genome shotgun sequence.
ACCESSION   DAOSCV010000523 DAOSCV010000000
VERSION     DAOSCV010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19895_length_3136_cov_2.587147"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLY56_12585"
     CDS             <1..681
                     /locus_tag="PLY56_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22360.1"
                     /translation="AARWARAAEKPEPRRPRPVTVPPPDPRSAGIPWKEDFSQGLREE
                     RWSVPRNAKSRIEVKDGRLEMELSSEETSAAITTRRTFDFGEQPLEFRWRFQFNHAGH
                     EWYLMQGVRLAPEASDDGYILFRIDPGIRVRLENADTPPSNFEFSLTPYESFATNVPH
                     QVRLVLTPERFFLEMDGKRLGEGEHDCHFTQAAITLSVSTGHKGRGDVVWWDDIEVVP
                     TRWPTEAP"
     gene            complement(687..3011)
                     /gene="ppsA"
                     /locus_tag="PLY56_12590"
     CDS             complement(687..3011)
                     /gene="ppsA"
                     /locus_tag="PLY56_12590"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOJ22361.1"
                     /translation="METIRWFTTLRKEDVLIGGGKGANLGELTAAGVPVPPGFVVTSD
                     AFNGFLRQSGLRDQILASLRNLDVNDTAALDGVAAELQRAVLAAEVPRQTVEEIHHAY
                     RELSQSSGVGEEFVAVRSSATAEDRPHTSFAGMNETFLNVRGADAVVEAVRRCWASLY
                     GARVIFYRREQGLAEEGMSIAVAVQKMINSEKAGVLFTENPATGDESVLVIESSFGLG
                     DAVVSGSVQPDYFEVRKKDLAILQRRIAHKSFADLRSESGENIRRELSDAEAEAPSLT
                     DEEVRAIAQLGLRIGEHYGTPQDVEWAVEGGQVYIVQSRPVTATGKAATSAEEETAPG
                     DGILVRGLGASPGIAVGRARVLASATEGARVQEGDILVTRMTAPDWAPVMRRAAAIIT
                     DEGGMTAHAAIVSRELGIPCIVGAGNATEKIPDGEIVTVDARQGIVYRGKRAETAAQQ
                     PRATAATVTAAPPVTATRLLVNLGEPDLAAEVAARPVDGVGLLRLEFLVLSVTNNVHP
                     RRLLEQGRGDEYRQKLAEGLRTFAAAFAPRPIICRSTDFRTNEYRGMEGGEAFEPREA
                     NPMIGYRGAFRYVAEPDLFRLELAAVRDVREKERLTNLHLMVPFVRTLGEFRRCKELI
                     DESGLTRDPAFQLWVMAEVPSILYRLADYAALGTYGVSIGSNDLTQLMLGVDRDSERL
                     APLFDERDDAVMGAIRDLIEGAHRVGMRCSICGQARSVYPEVTERLVEWGIDSISVNP
                     DAIDATRRIIASAEQRLLIAAARRVNPGAHPSDG"
BASE COUNT          513 a         1083 c         1044 g          496 t
ORIGIN      
        1 gcggcgcggt gggcgcgcgc ggcggagaag ccggagccgc gccggccacg cccggtgacc
       61 gtgcctcccc cggacccgag aagcgccggc atcccctgga aagaagactt ttcgcagggg
      121 ctccgggagg aacgatggtc cgtgccacgc aacgcgaaga gccggatcga ggtgaaggac
      181 ggccggctgg agatggagct ttcgtccgag gagacatcgg ccgcgatcac gacccggcgc
      241 accttcgatt tcggggagca gcccctcgag ttccgctggc ggttccagtt caaccatgcg
      301 gggcacgagt ggtacctgat gcagggcgtg cgcctggcgc cagaggcctc cgacgatggc
      361 tacatcctct tccgcatcga tcctgggatc cgggtgcgcc tcgagaacgc ggacacgccg
      421 ccgagcaact tcgagttctc gctgacgccg tatgagtcgt ttgccaccaa tgtgccgcat
      481 caggtgcgtc tggtactcac cccggagcga ttcttcctgg agatggatgg gaagcgcctc
      541 ggcgagggcg agcacgactg tcacttcacg caggcggcga tcacgctgag cgtctctacc
      601 ggccacaaag gccgcggcga cgttgtctgg tgggacgata tcgaggtcgt gcccacgcgc
      661 tggccaacgg aggccccctg accgtctcaa ccgtctgagg ggtgcgcacc ggggttcacc
      721 cgccgggcgg cagcgatgag cagccgctgc tccgccgagg cgatgatccg tcgcgtggcg
      781 tcgatggcgt ccgggttcac ggagatggag tcgatccccc actccaccag gcgctccgtg
      841 acctccgggt agaccgaccg cgcctgtccg cagatggagc accgcattcc cacgcgatgg
      901 gccccctcga tcaggtcgcg aatggcgccc atgacggcat cgtcgcgttc atcaaagagg
      961 ggggccaggc gctcgctgtc gcggtcgacg cccagcatca gctgcgtcag gtcattcgag
     1021 ccgatggaga caccgtaggt gcccaatgcg gcgtagtcgg caagccggta gaggatcgag
     1081 ggtacttcag ccatcaccca gagctggaat gccgggtcgc gggtgaggcc cgactcgtcg
     1141 atcagctcct tgcagcggcg gaactcgccc agcgtgcgca cgaagggcac catcaggtgc
     1201 aggttggtga gccgctcctt ctcgcgcaca tcgcgcacgg cggcaagctc caggcgaaag
     1261 aggtccggct ccgcgacgta gcggaacgcg ccgcgatagc cgatcatcgg gttggcttcc
     1321 cgcggctcga acgcctcgcc accctccata ccgcgatact cgttggtccg gaaatcggtg
     1381 ctccggcaga tgattggcct gggcgcgaac gccgccgcga aggtgcgcag gccctcggcc
     1441 agcttctggc ggtactcatc gcccctcccc tgctccagca accggcgggg gtggacgttg
     1501 ttggtcaccg agagaacgag aaactccaag cggaggagcc cgacgccgtc caccgggcgc
     1561 gcggctactt cggccgctaa gtccggctcg cccaggttca ccagcaggcg cgtcgcggtg
     1621 acgggtggcg ccgcagtcac cgtggcagct gtggcgcgcg gttgctgagc ggcggtttcg
     1681 gccctcttcc ctcggtagac aatcccctgc cgggcatcga cggtcacgat ctccccgtcc
     1741 gggatcttct cggtggcgtt gcccgcgccg acaatgcagg ggatccccag ctcgcgcgag
     1801 acgatggcgg cgtgcgcggt catcccgcct tcatcggtga tgatcgccgc agcccggcgc
     1861 atcacgggcg cccagtccgg cgcggtcatg cgcgtcacca agatgtcgcc ctcttggacg
     1921 cgcgcgccct cggtagcgga ggcgagcact cgcgcgcgac ccaccgcgat ccccggcgag
     1981 gcccccaggc cacggaccag gatgccatcc cctggtgcgg tctcttcctc ggcggacgtg
     2041 gccgccttcc cggtagccgt caccgggcgc gattggacaa tatagacctg gccgccctca
     2101 accgcccatt ccacatcctg aggcgttccg tagtgctcgc cgatccgcag gcccagttga
     2161 gcgatcgcgc gcacttcctc atcggtgagg gagggtgcct ccgcctcggc gtcggatagc
     2221 tcccggcgga tgttctcgcc actctcgctg cgcaagtcgg caaagctctt gtgggcgatc
     2281 cgccgctgga ggatcgccag gtcctttttg cgcacctcga agtaatcggg ctggacactc
     2341 ccgctcacga ctgcatcgcc caggccgaag gaggactcaa tgacgagcac gctctcgtcg
     2401 ccggtggcgg ggttctcggt gaagagcacc cccgccttct cggagttgat catcttctgg
     2461 accgcgacgg cgatgctcat tccctcttca gccaggccct gctccctgcg gtagaaaatc
     2521 acccgcgcgc catagaggga ggcccaacac cgtcgcaccg cctcaaccac cgcatcggcg
     2581 ccgcgcacat tcaagaaggt ctcgttcatt cccgcgaaag aggtgtgcgg ccggtcctcc
     2641 gcagtggccg acgaacgcac ggccacgaac tcctcgccca cgccggaact ctgcgagagc
     2701 tcccgatagg cgtggtgaat ctcctccacc gtctgccggg gcacctcggc cgcaagcacg
     2761 gcgcgctgta gctccgcggc cacgccgtcc agggcggcgg tatcgttcac atcgaggttg
     2821 cggaggctgg caaggatctg gtcgcgcagg ccagactggc ggagaaagcc gttgaaggca
     2881 tcagacgtga ccacgaaccc ggggggcacc ggcactccag cagcggtcaa ctcgcccagg
     2941 ttggcgccct tccccccgcc gatgagcaca tcctctttgc gcaacgtagt gaaccatcgg
     3001 atcgtttcca cggaagagag gttacccgag ggtggtaccg ggcaaacccc ggccaggcgc
     3061 cacaccgcga ggatcgggcg gcgcgcgagg ttcaggggtg gcggtcgcga aggttctgca
     3121 tctcgagcgc ggtgcg
//
LOCUS       DAOSCV010000524         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19904_length_3135_cov_2.720455, whole genome shotgun sequence.
ACCESSION   DAOSCV010000524 DAOSCV010000000
VERSION     DAOSCV010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19904_length_3135_cov_2.720455"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="PLY56_12595"
     CDS             <1..1138
                     /locus_tag="PLY56_12595"
                     /inference="COORDINATES: protein motif:HMM:NF005928.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ22362.1"
                     /translation="DPARVVDQFVALPGAKSILAHGPTKGTIVTAEHLQIDLRVVKPE
                     EYGAALQYFTGSKAHNIHLRALAEAAGYKVNEYGVFRTSDDRRVAGETEKGVYQTVGL
                     EWIPPELREDQGEIEAAASGDLPDLVELADIRGDLHLHTDWSDGRDSLEQMVEAARAR
                     GYEYVVISDHSVSMGFVHGLTLERIQEQRGRIAELNERYPDIRILHGTEVNIRADGSL
                     DYEDEVLAEFDVVTASVHSGMGMDRERMTRRILRAVRNPHVDILGHPTGRILGKREPY
                     EVDLQAVIRAAAESGTALEINAQPDRLDLKDTDARLVAEAGAMVAINSDAHSVEQLGL
                     LEYGIATARRGWIERKHVLNALPLEQLLRRLGRPTTPARYALAA"
     gene            complement(1271..>3135)
                     /locus_tag="PLY56_12600"
     CDS             complement(1271..>3135)
                     /locus_tag="PLY56_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ22363.1"
                     /translation="EAWPSYTGLDIIGIESSHFRDRALMAYHRYGGYQKPVLTMNFIN
                     LHKLDDRPTAEEFVLASAQFGHFPSTGRMVREGYRDYGDVCHTYYPALKEMSEAGWEP
                     EPLVTGSYAERFGRGEVVYLTLRAPVEGRQVELTLLNEARAGARGPVVVVDAVQLAPV
                     EFTPTQEGIRIRLVQGAEELTILRISSRENAERWLLERAARHCENAAIVRGRSENTDR
                     LKALGVSVRGLKAGDAAGFGKAVAAIRAEKAKVLAEEESLERTSITTELLDAERALAE
                     WVLFTGNARLTFEGEGLRPVTEVAAITPRFQPGKSGATLVSAWAGQERNILRWVSATP
                     PAGAVSAGQAVSIQRDLPGAMHLCAALSVPVAGQEPISVYRVVNIHFTRVVSVQVKKA
                     LSEDRQSLRYILDVERLTGPMPLRVKAEAEGIAIEPRERVLGPEEKSCEFRVNLSRET
                     TEVKDVSFRIETAAGKPIATALASKGAKVTADSSYSRYRPEPAIDGVWDTSRLAWWDK
                     AWASRDTAGTEHWLEVQLPQPMPLSEVWIYWALDDNQIFASRNYDVEIWENNAWKSVV
                     QVRNAPVTTVSQHAWPVTTTSRVRIRQLPGGGPAQRPHIMWISEVCLYNRGTLN"
BASE COUNT          536 a         1087 c         1013 g          499 t
ORIGIN      
        1 tgaccccgcg cgcgtcgtgg accagtttgt ggcgcttcca ggcgcgaaga gcatcctcgc
       61 ccatggaccg accaagggca ccatcgtcac cgccgagcac ctgcagatcg acctgcgtgt
      121 ggtaaagccc gaggagtatg gggcggcact gcagtacttc accggctcga aggcgcacaa
      181 catccacctc cgcgccctcg ccgaggcggc aggctacaag gtgaacgagt acggcgtctt
      241 ccgtacctca gacgaccggc gcgtcgccgg cgagacggag aagggcgtct atcaaaccgt
      301 tggtctggag tggatccccc cggagctgcg cgaagaccag ggcgagatcg aggcggccgc
      361 gtccggcgac ctcccggatc ttgtggagct cgcggatatc cgcggcgacc tgcacctgca
      421 caccgactgg agcgatggtc gcgattccct cgagcagatg gtagaggcgg cgcgcgcgcg
      481 cggctacgag tatgtggtaa tctccgacca ctccgtctcg atgggcttcg ttcatggcct
      541 cacgctggag cggatccagg agcagcgcgg gaggatcgcg gagctcaacg agcgctatcc
      601 cgatatccgc atccttcacg gcacggaagt caacatccgc gccgatggca gcctcgacta
      661 cgaagatgag gtcctggcgg agttcgacgt ggtcacggcg tcggtacaca gcggcatggg
      721 gatggatcgc gagcgcatga cccggcgcat cctccgggcg gtgcgcaacc cgcacgtgga
      781 cattctgggt caccccaccg ggaggatcct cgggaagcga gagccctatg aggtggatct
      841 acaggcggtg atccgcgccg ccgccgaaag cggcaccgct ctggagatca acgcgcagcc
      901 cgaccgcctc gatctgaagg acacggacgc ccggctggtg gccgaagcgg gcgcgatggt
      961 cgccatcaac tccgacgccc actccgtcga gcagctcggc ctcctggagt atgggatcgc
     1021 caccgcccgg cgcggctgga tcgagcggaa acacgtgcta aacgcgctcc cgctggagca
     1081 gctgttgcgc cgattgggcc gcccgaccac gccggccaga tatgccctgg ccgcgtagcg
     1141 ccgacgcccg gcaccctcat cgagggtatc gctgggggat gcgatcagcg gcatgaatcg
     1201 atgtggcgcg cgggcggtag cgcccgcgcg ccacatccga gagtagatac tctacgtggt
     1261 cacgccgcga tcagttgagc gtgccccggt tgtagagaca gacctccgag atccacatga
     1321 tgtgcggccg ctgggctggc ccgcctcccg gaagctggcg gatgcgcacc cggctggtag
     1381 tcgtgaccgg ccaagcgtgc tgggagaccg tcgtcacggg cgcgttccgc acctggacga
     1441 cgctcttcca ggcattgttc tcccaaatct ccacgtcata gttgcgcgac gcgaagatct
     1501 ggttgtcgtc tagcgcccag tagatccaca cctcggagag gggcatcggc tggggcagct
     1561 ggacctcgag ccagtgctcg gtaccggcgg tatcgcgaga cgcccacgct ttgtcccacc
     1621 aggcaagccg cgaggtatcc cagacgccgt cgatcgccgg ctcgggccgg tagcgcgagt
     1681 aggagctatc cgcggtcact ttcgcgccct tacttgccag ggccgtagca atcggcttgc
     1741 ccgccgcggt ctcgatccgg aacgagacgt ccttgacctc cgtggtctcg cgggacaggt
     1801 tcacccggaa ctcgcagctc ttctcttccg ggccaagaac gcgctcgcgc ggctcgatgg
     1861 cgatgccttc agcctcggcc ttcacccgaa gtggcatcgg cccggtgaga cgctccacgt
     1921 cgaggatgta gcgaaggctc tgccgatcct ccgaaagcgc cttcttcacc tgaacgctaa
     1981 ccacgcgcgt gaagtggatg ttgaccacac ggtagacaga tattggctct tgccccgcga
     2041 caggcaccga aagcgctgcg cacaggtgca ttgccccggg aagatcgcgc tggatggaga
     2101 cggcctggcc cgcggatacc gcgccggccg gcggggtggc actcacccac cgcaggatgt
     2161 tccgctcctg cccagcccac gcgctgacga gcgtcgcgcc gctcttgccc ggctggaacc
     2221 ggggcgtgat cgccgcgacc tcggtcaccg gccgaaggcc ctctccctca aacgtcagtc
     2281 gggcattccc cgtaaagagg acccactcag caagcgcacg ctcggcatcc aggagctccg
     2341 tggtaatgct cgttcgctcc aggctctcct cttccgcgag caccttcgcc ttttccgcgc
     2401 gaatggcggc gaccgccttg ccaaagccgg cggcatcgcc ggctttcagg ccgcgcaccg
     2461 agacgccaag cgccttcagc cggtcggtgt tctccgagcg cccgcggaca atcgccgcgt
     2521 tctcgcaatg ccgggcggcg cgctccaata gccagcgctc cgcattctcg cgcgacgaga
     2581 tgcgcaggat cgtcagctct tccgcaccct gcaccaggcg gatgcggatc ccctcctgcg
     2641 tgggggtgaa ctccaccggc gcgagctgca cggcatccac caccaccacg gggccgcgcg
     2701 cgccggcccg cgcctcgttc agaagcgtca gctccacctg ccggccctca accggagcgc
     2761 ggagggtgag atagaccacc tcgccccgcc cgaagcgctc ggcgtagctg ccggtcacca
     2821 gcggctccgg ctcccagcct gcctcggaca tctccttcag cgccgggtaa taggtgtggc
     2881 acacatcgcc atagtcgcgg tagccctcgc ggaccatgcg gccggtggag gggaagtgac
     2941 cgaactgcgc gctggccaga acgaactcct cggccgtcgg ccgatcatcc agcttgtgga
     3001 ggttgatgaa gttcatcgtg aggaccggct tctggtagcc cccatagcga tggtaggcca
     3061 tcaacgcccg atcccggaag tggctgcttt caatgccgat gatatccagg cccgtatagc
     3121 tcggccaggc ctcgg
//
LOCUS       DAOSCV010000525         3131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19942_length_3131_cov_3.118010, whole genome shotgun sequence.
ACCESSION   DAOSCV010000525 DAOSCV010000000
VERSION     DAOSCV010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19942_length_3131_cov_3.118010"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PLY56_12605"
     CDS             <1..1095
                     /locus_tag="PLY56_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HOJ22364.1"
                     /translation="SAELPGTGGRRGAGDGSGSGLGTGSGRGIAKARPGLEGIPFGDP
                     GGTPAGGNPAGGGGKGGGPGGPGHGIQMARRGTGSGGGDGGEDGAGIWPGRGAGSGGI
                     GAGAGAGKRAGGSGTGPGTGTGKGSGAGTHPGSGSGARGGGGELPGKSGGKAAKPGLP
                     RYTKAVPGLEEWDEEVLGRGFYPDGIMGYYYDDPDQTVDHANPESYLNTPIPEGATFT
                     RLVAKRKDKRIDFNWNPAGEWGDWSKLLNPLPGINNVYWSARWIGQLFVPKDDVYTFK
                     FDPLDDGGRLYLRYRPGGPLVKVIDAWRVSSTRPETRPHRMQRGAYDIQVEYAQRPEF
                     YAAISLKWKSSSFDWEVIGPYRSGGSRRAR"
     gene            complement(1188..1988)
                     /locus_tag="PLY56_12610"
     CDS             complement(1188..1988)
                     /locus_tag="PLY56_12610"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ22365.1"
                     /translation="MKFGLIHYNAPGANLEEFLRYAVDTGFDCTELQCRDVWPEGEEH
                     PERRAEEVRALAERLGIAISAFSAGNDFVVLEEDVVAAQVARMKRICDLAEILGTKVI
                     RTEGGQPKESVPEDRWVDAMAGCFRRCLEFAEPRGIRFAVDNHGYVTNAPGILPALFE
                     AVPSPSVGTNLDTMNYRWWGNSVEECDRLYEQVASKTFHTHLKDGTGSRKEYVGAALG
                     EGEIHLDHAVRTLKAAGYQGPWIAEYEGRTDSAEGYRKCLEWMKAHID"
     gene            2421..2780
                     /locus_tag="PLY56_12615"
     CDS             2421..2780
                     /locus_tag="PLY56_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22366.1"
                     /translation="MRSPVIWGAIAAVLAAVTTGVLLGSAGGAAWSGEAVQAAMRLYA
                     RLADAGRQYHLGFLLPVIAVTVSFHVGTRAGHRLRGRSEARAAAAPHAPVVRYTLLFC
                     LSAVAMLVWAASEGLLP"
     gene            complement(2793..>3131)
                     /locus_tag="PLY56_12620"
     CDS             complement(2793..>3131)
                     /locus_tag="PLY56_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22367.1"
                     /translation="TIAVEEYGPKGMRIAICGGRPNIRFDQRGAVSMTPTFPQKARIV
                     ISDGRYRIRPGTRHLLRAKDLASGAVSQYQVVVGADAKIEIEKEFYSDSLTLTPLRPA
                     EERPSASPSH"
BASE COUNT          516 a         1029 c         1058 g          528 t
ORIGIN      
        1 tccgccgagc ttcccggaac cgggggccgg cgaggcgccg gcgatggatc cggcagcggc
       61 ctcggcaccg gctccggtcg agggatcgcc aaggcccggc ccggcctgga agggatcccc
      121 tttggcgatc ccggcggcac gcccgcgggg ggcaatcccg ctggaggcgg cggcaaggga
      181 ggcggtccgg gcggcccagg tcacggcatt cagatggcgc gccgcggcac cggctctgga
      241 ggcggcgacg ggggcgaaga cggcgcgggc atctggccag gccggggcgc tggcagtggc
      301 ggcatcggcg caggcgcggg ggctggcaag agagcgggcg gcagcggcac cggcccgggc
      361 acgggaaccg gcaaaggatc gggcgccggc acgcacccag gctccggatc cggcgcgcgc
      421 ggcggtgggg gcgagcttcc cgggaaatca gggggtaagg cggccaaacc tggcctgccg
      481 cgctacacga aggcggttcc cggcctggag gagtgggatg aggaggtcct cgggcgaggc
      541 ttctatccag acggcatcat gggctactac tacgacgacc cggatcagac cgtggatcac
      601 gcaaatcccg agagctacct gaatacgcca atcccagagg gcgctacttt cacgcgcctg
      661 gtcgcgaagc gcaaagacaa acggatcgac ttcaactgga accccgcggg agagtggggc
      721 gattggtcga agctcctcaa cccgcttccc gggatcaaca acgtctattg gagcgcgcgc
      781 tggatagggc agctcttcgt gcccaaggat gatgtctaca ccttcaaatt cgatcccctg
      841 gatgacggcg gccggctcta cctgcgttac aggccgggtg gcccactggt gaaggtgatc
      901 gacgcctggc gggtttcgtc gacgcgcccg gagacacgcc cccaccggat gcaacgcggt
      961 gcctacgaca tccaggtgga gtatgcgcag cgcccagagt tctatgcggc gatctcgttg
     1021 aagtggaagt ccagctcatt cgattgggag gtcatcgggc cttaccgatc cggtggcagt
     1081 cggcgtgcgc ggtagcaagg atgtaggtcc ggccgccacg gcccgaaagc gagtggccgg
     1141 ggagcacggc acaggcgctg ccctgctccc cggctcgtga tccgtggtta gtcgatgtgt
     1201 gccttcatcc actccaggca cttgcggtag ccttcggcgg aatcagtgcg cccctcgtac
     1261 tcggcgatcc acggtccctg gtaaccagcc gccttgagcg tgcgcacggc gtgatcgagg
     1321 tgaatctcgc cctcgccaag cgccgccccc acgtactcct tgcgcgagcc ggtgccatcc
     1381 ttcaggtggg tgtggaacgt cttgctggcc acttgctcat agaggcggtc gcactcctca
     1441 accgagttgc cccaccagcg atagttcatc gtatcgaggt ttgtgccgac actcggcgag
     1501 ggcacggcct cgaagagtgc ggggagaatg cctggggcgt tggtgacgta gccgtggtta
     1561 tcgacggcga agcggatccc cctcggctcg gcgaactcca ggcagcgccg gaagcagccg
     1621 gccatcgcat ccacccagcg atcctcggga acgctttcct tcggctgacc gccctcggtg
     1681 cggatcactt tggtgcccaa gatctccgcc aggtcacaga tccgcttcat gcgggctacc
     1741 tgcgccgcaa cgacgtcctc ttccagcacg acaaagtcat tccccgcgga aaacgccgag
     1801 atggcaatcc cgagacgttc ggccagggcg cggacctcct ctgcgcggcg ctcggggtgc
     1861 tcttcaccct ccggccacac atcgcggcac tgtagctccg tgcagtcgaa gccggtatcg
     1921 acggcatagc ggagaaactc ctccaggttg gctcccggtg cgttgtagtg gatcaatccg
     1981 aacttcatcg gcatcctctc ttcccggatc agaatcccta tctgcggcgc cggcaagcgc
     2041 gcgcacggca accgcttgtc tcccttagtt cgccctggat gccctccctc ctggcgcgga
     2101 tggagagtgc gagctctctg ggttgtacga cccctctcgg ggttgggatc ggaccatccg
     2161 catgcctcgc tcggaggtgt caattccttt acacgcaaaa cagttgaatg gtataatgcg
     2221 ctcggcacca ccctggaagc acctacctgg gtcgcgcagc gcctgaattc acgggcttcc
     2281 ggggatgccg gcgccaacaa cgcaccgaag gcgcattcca cgacagaccg agagccctgc
     2341 tcctcgcggg gccccatcca gcggcaacac gtcacggtgt cgcctttgtc catagatgga
     2401 gtggagggag agacgtgagg ttgagatcac cggtcatttg gggagcaatc gcggcagtgc
     2461 tggcggcagt tactacaggc gtcctgctcg gatcagcggg gggcgccgcc tggagcgggg
     2521 aagcggtgca ggcggccatg cgcctctacg cgcgcctcgc cgacgcggga cggcagtacc
     2581 acctcgggtt tctcctcccg gtgatcgcgg tgaccgtcag cttccatgtc ggcacgcgcg
     2641 ccgggcatcg cctccgcggg cgctccgaag cgcgggcagc cgccgcgcca cacgcgccag
     2701 tcgtccgcta tacgctcctc ttctgccttt ccgctgttgc catgctcgtt tgggcggcct
     2761 ctgagggcct cttgccgtaa ccgcccgcgt gatcagtggc tgggagaggc cgacggcctc
     2821 tcctcggccg gcctgagggg cgtgagggtg agggaatcgg agtagaactc cttctcgatc
     2881 tcgatcttcg cgtcagcgcc cacgaccacc tggtactggg acacggcgcc gctggcaagg
     2941 tccttcgcgc gtaggaggtg ccgcgtgccc gggcggatac gatagcggcc gtcgctgatg
     3001 acaattcgcg ccttctgggg aaatgtgggc gtcattgaga cagcgccccg ctggtcgaag
     3061 cggatattgg gccttccgcc gcagatggcg atccgcatcc ccttcgggcc atactcctca
     3121 acggcgatgg t
//
LOCUS       DAOSCV010000526         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19958_length_3129_cov_3.884841, whole genome shotgun sequence.
ACCESSION   DAOSCV010000526 DAOSCV010000000
VERSION     DAOSCV010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19958_length_3129_cov_3.884841"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PLY56_12625"
     CDS             complement(<1..766)
                     /locus_tag="PLY56_12625"
                     /inference="COORDINATES: protein motif:HMM:NF021449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HOJ22368.1"
                     /translation="MSPSTRERRPAPPAHETELYPPIRDFLVSLGYTVRSEVRGCDIT
                     ATREDELVVIEMKRRFSTDLLIQAVERQRLTDSVYVALPQSAIPTGRSGRARWQGMQR
                     LLKRLELGLLLVSSESSVLVLFHPIACERRRAGRARRALLREVEGRSGDYNVGGSTRQ
                     RICTAYREAALFIACSLERHGPMRPRDLRALGADARTQAILSKNYYGWFERIDRGVYA
                     LKPQGHAALAEYPELAARFRHALSEREAGRAGERRLP"
     gene            complement(763..1761)
                     /locus_tag="PLY56_12630"
     CDS             complement(763..1761)
                     /locus_tag="PLY56_12630"
                     /inference="COORDINATES: protein motif:HMM:NF020448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HOJ22369.1"
                     /translation="MLHIHCGDSSAGTLRQSDVPGEVIVWCDPVVQGPTPAGLSGEAW
                     HRRRAEYLATTAARLTAADLARRLQAEDDALARAPEHDEVVLWFDACLFDQSILICVL
                     DRLSRLALGETRLSLICIGDFPGFARFRGLGELHAEQLASLFPTRRPVTPEQIALARR
                     AWEAWVAPDPTDLAALARAETALLPYLGEALARRLEQFPSTTNGLDRLEQEALEAIAE
                     GHDRPFSLFRAVDEREARPFIGDTMLWCLLEELASAETPLVTIDGPGPLPRFDDPPTS
                     LEQWRLSLTPAGRAVLAGREDRIRLNGIDRWVGGVHLAGRGPIWRWHPREQKLVLA"
     gene            1965..2780
                     /locus_tag="PLY56_12635"
     CDS             1965..2780
                     /locus_tag="PLY56_12635"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOJ22370.1"
                     /translation="MMRIFIRGSGRAARGRAGVPLCWIVGLLIALGVLSGASLHAGPA
                     PPDVNAVLAKLDDLYKSSGSIGRVEMTVVRPGSTRTLVMRVWTRGEDRALIIIDSPAR
                     EAGTATLRVGQNLWNYLPRIARTIRIPPSAMLSPWMGSDFTNDDLVQESSYREDYNAR
                     ITGRSTDPPGWQLRLDAKPDTVGLWRRIDLVVSDDGWLPVVERHYDRRGDLARVMRFD
                     QVRVMGGRRIPTRVEVTPTGKPGHRTVMRYLEMQFNANVPESTFSLQRLEQNR"
     gene            2791..>3129
                     /locus_tag="PLY56_12640"
     CDS             2791..>3129
                     /locus_tag="PLY56_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ22371.1"
                     /translation="MRSSTLRLAWRNLGRNRKRTGLALAAIGLGQFALLAMSGITRGY
                     TDAMLETVTGPMVGHVQIHAPGWRDEQSLELSVTALDARLDALREEPGVARASARIYA
                     PALVAVTRDAQ"
BASE COUNT          497 a         1017 c         1142 g          473 t
ORIGIN      
        1 gtggcaggcg ccgttcaccg gcacgtccgg cttcgcgctc gctcagcgcg tgccggaagc
       61 gcgcggccaa ctcgggatac tccgcgaggg cagcgtggcc ctggggcttc aaggcataga
      121 cgccccggtc gattcgttca aaccacccgt agtagttctt gctcaggatc gcttgcgtcc
      181 tggcatcggc accgagagcg cgcaggtcgc gcgggcgcat ggggccatgc cgctccagag
      241 agcaggcgat gaaaagggct gcctcccgat aggcggtgca gatcctctgg cgggtgctgc
      301 ctcccacatt gtagtcgccc gagcggccct cgacctcgcg caacagcgcc cgccgggccc
      361 ggcccgcgcg ccggcgctcg caagcaatgg gatggaaaag caccaagacg ctgctctcgc
      421 tggagacgag aaggagtccg agctccaacc gcttgaggag acgctgcatc ccttgccagc
      481 gtgcccggcc cgagcgtccg gtggggatcg cggattgtgg caaggcgacg tagaccgagt
      541 cggtcaggcg ttgtcgctcc accgcctgga tgaggagatc ggtggagaat cgccgcttca
      601 tctcgatcac caccagctca tcctcgcgcg tggcggtgat gtcgcacccg cgcacctcgc
      661 tgcgcacggt gtagccaagc gacaccagga agtcgcgaat cggcgggtag agctcggtct
      721 cgtgcgccgg gggcgccggc cgcctttccc gcgtggacgg gctcatgcca ggacgagctt
      781 ctgctctcgc gggtgccagc gccagatcgg gccccgtccg gccaggtgga ccccacccac
      841 ccagcggtcg atgccgttga ggcgaatccg atcctccctg cccgccagta cggcgcggcc
      901 agccggggtg agtgacaagc gccactgctc gagggatgtg ggcgggtcat cgaagcgcgg
      961 gagcggcccg ggaccatcga tcgtgacgag cggcgtttcg gcggacgcga gctcctccag
     1021 gaggcaccag agcatcgtat ccccgatgaa cgggcgcgcc tcgcgctcgt ccacggcgcg
     1081 gaagagggag aaggggcggt cgtgcccctc ggcgatggct tccagcgcct cctgctccag
     1141 gcgatccagg ccgttggtgg tggacggaaa ctgctccagg cggcgggcca gcgcctcgcc
     1201 caggtaggga agcagggcgg tttctgcgcg cgccagggcc gccagatcgg tggggtcggg
     1261 cgcaacccag gcttcccacg cccgccgcgc cagcgcgatc tgctcgggcg tgacagggcg
     1321 gcgcgtgggg aagagggatg cgagttgctc cgcgtgcagc tcgcccaggc cacggaagcg
     1381 cgcgaagcct gggaagtctc caatgcagat aaggctaagc cgcgtctctc caagcgcgag
     1441 ccgcgagagc cggtcgagca cgcagatgag gatcgactgg tcgaagaggc aggcatcgaa
     1501 ccacaggacc acttcgtcgt gctcgggcgc gcgtgccagg gcgtcgtctt ccgcctggag
     1561 acgccgcgcc agatccgcgg cggtgagccg ggcggctgtg gtcgcgaggt actccgcgcg
     1621 ccggcggtgc cacgcctctc ccgagagacc cgcgggcgtc gggccctgga ccacaggatc
     1681 acaccagacg atcacttcgc ccggcacgtc gctctggcgc agcgttcccg cggaggaatc
     1741 tccgcaatgg atgtgaagca ttctggacct ccttcgggaa gacggggata tcgacggctc
     1801 acgcacggag tgaggttctc tccccgagca gaggagtcct cccatccgtc cgaggctccc
     1861 gggtggcctg gaatctgcgg gccgaccggg ttgatgcgag aggacacgtt cgttcccggt
     1921 tgaaccggga tgagcgaggg cgcatcctgg catcgaggga ggcgatgatg cgaatcttca
     1981 ttcgcggatc agggagagcg gcgcgcgggc gtgccggcgt gccgctctgt tggatagtcg
     2041 ggctgctgat tgccctgggg gttttgtcgg gcgcgtcact ccacgcgggc ccggcgccac
     2101 cggatgtgaa tgccgtgttg gccaagctgg acgacctcta taagtcgagc gggagcatcg
     2161 ggagggtgga gatgacggtg gtccgccccg ggagtacgcg cacgctggtg atgcgcgtct
     2221 ggactcgggg ggaagaccgc gcgctgatca tcatcgattc gcccgcgcgc gaggcgggaa
     2281 ccgcgacgct gcgcgttggt cagaacctct ggaactacct gccacgtatc gcgcggacga
     2341 ttcgcatccc gccctcggcg atgctcagcc cctggatggg gagcgatttc accaacgatg
     2401 atctggtgca ggagtcgtcg tatcgggagg attacaacgc ccggatcacc ggtcgctcca
     2461 cggacccgcc aggttggcag ctccgcctcg acgcgaagcc ggacacggtg ggcctctgga
     2521 gacggatcga tctggtggtt tcggacgacg gttggctgcc ggtggtcgag aggcactatg
     2581 accgccgggg cgacctggcg cgggtgatgc gcttcgacca ggtgcgcgtg atgggcggcc
     2641 ggcgcatccc gacgcgcgtg gaggtgacgc cgaccggcaa gccgggccat cggacggtga
     2701 tgcgctacct ggagatgcag ttcaacgcga acgtgcctga gagcaccttc agcctgcagc
     2761 gcctcgaaca gaaccggtga ggagagtggg atgcgctcga gcacgctgcg cctggcatgg
     2821 cgcaacctgg gccgcaaccg caagcgcacc ggcctggcgc tggccgccat cggcctgggg
     2881 cagttcgcgc tgctggcgat gagcgggatc acccggggct acaccgatgc gatgctcgag
     2941 acagtcaccg gtccgatggt gggccatgtg cagatccacg cgcccgggtg gcgcgatgag
     3001 cagagcctgg agctgtcggt aaccgcgttg gatgcccggc tcgacgcgct ccgcgaggag
     3061 cccggcgtgg cgcgtgcctc ggcgcggatc tacgcgcctg ccctggtggc cgtcacgcgc
     3121 gatgcccag
//
LOCUS       DAOSCV010000527         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_19979_length_3127_cov_3.271484, whole genome shotgun sequence.
ACCESSION   DAOSCV010000527 DAOSCV010000000
VERSION     DAOSCV010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19979_length_3127_cov_3.271484"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /gene="nth"
                     /locus_tag="PLY56_12645"
     CDS             <1..506
                     /gene="nth"
                     /locus_tag="PLY56_12645"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ22372.1"
                     /translation="VTIRVAPALLDRARTPEEMLAIPEEELATLLSPATFAETKARHI
                     RQISALLLERHGGCVPSDARALRALPGVGPKVANIVLATAFHQPAIAVDIHVHRITRR
                     WGYTDAPTPEKTEEQLRAKLPREYWVEINERLVPFGKFVCTGQRPRCNDCPLLTLCRQ
                     VGVADHR"
     gene            complement(513..1736)
                     /locus_tag="PLY56_12650"
     CDS             complement(513..1736)
                     /locus_tag="PLY56_12650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOJ22373.1"
                     /translation="MSVNAVEWLGVVAVVTLVGVAGALGHVVLASELGLLSYLVLGLI
                     AVVIALGIGHVAVEVGRRHQREIKRHNEELAALNAVAVTASRSLELTAVLRDCLEKTL
                     EVMGLDAGAVFLASQGEIGSLAAHRGLTDHFLDGLARMKMGENVPGSVILPDSPLLTP
                     DQEGETHREWIRASVSVPMRSEGRVVGTMYLFARGHRDFSQREVQILVAIGDVIGMAV
                     QNAKLHAQVIELAARDPLTGLYNRRRFEEVYRQEVARARLYRAPLTVAMIDVDCFKEV
                     NDRFGHAVGDRALEAIARILLKGRAMDVAARYGGDEFVVLMPNTELQGAQVVADRIQR
                     AARAVVFPDIPYYRLSLSIGLADSSAGYEGILERADAQMYACKRGGANHPLSTPRSER
                     PGPAKMEHGGLPRAC"
     gene            complement(2468..>3127)
                     /locus_tag="PLY56_12655"
     CDS             complement(2468..>3127)
                     /locus_tag="PLY56_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22374.1"
                     /translation="LAGIDIRALNASVSFTGPKPTVTVPLPAGLIVTGLSSRQIPADA
                     LAVVFFNEQRGAWELVPGAIVNVTSTQVTFAIGESTGTYALIVPATWTLPAPVQRGEN
                     VVFPPIAGFNGYVSIGRISQSLDFPTVSEPVETLLLSAYAALTGEVPNGPFVVQVPPG
                     TTTGRSASEVTATVSVNFGTFGTVQGTRTTWQVEDVEVPEAPEIPPHLQGHVQGGGVG
                     G"
BASE COUNT          566 a         1105 c          975 g          481 t
ORIGIN      
        1 aagtgaccat tcgcgtggcc ccggcgctcc tcgaccgggc gcgcacgccc gaggagatgc
       61 tggccatccc cgaagaggaa ctggccacgc tcctctcccc cgctactttc gcggagacga
      121 aggcacgcca catccgccag atcagcgcgc tcctgctgga gagacatggt ggctgtgttc
      181 cgagcgacgc cagggcactg cgcgccctcc cgggggtcgg ccccaaagtg gcgaacatcg
      241 tcctggccac cgccttccac cagccggcca tcgccgtcga tatccatgtc caccggatca
      301 cgcggcgctg gggctacacg gatgcgccca ccccggagaa gaccgaagag cagctacgcg
      361 cgaaactgcc gcgggagtac tgggtggaga tcaacgagcg cctggtacct ttcgggaagt
      421 tcgtgtgtac cggccagcgc ccgcgctgca acgactgtcc cctgttgaca ctctgccgcc
      481 aggtgggagt ggctgaccac cggtaggctc ccttaacacg cgcgcggaag ccctccgtgt
      541 tccatctttg ccggtccggg gcgttctgat cggggagtgg agagcggatg gttggcccca
      601 ccccgcttgc aggcatacat ctgcgcgtcc gcgcgctcca gaatgccctc atagccggca
      661 gagctatctg ccaggccgat gctgaggctg aggcgatagt acgggatatc tggaaacacc
      721 acggcacgag cggcgcgctg gatccgatcc gcgaccacct gagcgccctg cagctccgtg
      781 tttggcatca ggacgacaaa ctcatcgccg ccgtagcgcg cggcaacatc catcgcgcgg
      841 cccttgagga gaatcctggc aatcgcttcc agcgcccgat ccccgacggc atgcccgaaa
      901 cgatcgttga cctccttgaa gcagtctacg tcgatcatcg cgacggtcag gggcgcccga
      961 tagaggcggg cacgcgccac ctcctggcgg tagacctcct cgaatcggcg gcgattgtat
     1021 agccccgtga gtgggtcgcg cgcggcgagt tcgatcacct gtgcatgcag cttcgcgttc
     1081 tgcaccgcca tgccgatcac gtcgccgatg gccaccagga tctgcacctc tcgctgggag
     1141 aaatcacgat ggccgcgcgc aaagagatac atcgtcccca ccacgcggcc ctcggagcgc
     1201 atcggcacgc tcaccgatgc ccggatccac tcgcgatgcg tctcgccctc ctgatccggg
     1261 gtaagcaagg gggagtctgg caaaatcacc gatccgggaa cgttctcgcc catcttcatt
     1321 cgggcgaggc catccaggaa gtgatccgtc agaccacggt gcgccgccag cgatccgatc
     1381 tctccctgcg aggccaggaa gaccgcaccc gcgtcgagtc ccatcacctc gagcgtcttt
     1441 tccagacaat cacggagcac ggcagtgagc tcgagtgacc ggctcgcagt caccgcaacc
     1501 gcgttcagcg ccgcgagttc ctcgttgtgc cgcttgatct cccgctggtg acgccgaccc
     1561 acttccacgg ctacatgacc aatgccaagg gcgatcacga ccgcgatcaa gccaaggacg
     1621 aggtaactca gcaggcctaa ttcacttgcg aggacgacgt gccctaacgc cccagccaca
     1681 cccaccagag tgacgaccgc cacaaccccc aaccactcca ccgcgttgac actcataccc
     1741 gtttccaggt ggcgggcgtc gctggcccgg cggaccgcac gccgtgagcg cccctcccgc
     1801 gccaaaggct ccgcgcgcgc cgggaggcac acccaaaatg atccgttcgc cacctctcag
     1861 cggactcctc tccatcgcgc gcggtggcag ctcacggaag aaggcggggg cacattcggg
     1921 gggcatcccc tgcttcaggc cagcggggta tcctctcaca tcggaacggg gaaccgcaga
     1981 cttcccgcgc ttccgcagca ggcgctcata gtgtgggctc gcaaggcccg ggcgcacggg
     2041 tggcaggctc ctggccagcg tgccacaccc gcgcggcggc cgcgagcgtg tgtaacggcg
     2101 tcgccgcgcg cctggccatg ccctgaccaa actcccgcag gcgggcgctc gtctctttcc
     2161 cccgccggga ggcgagaaag agtcctgcca tcgcacccgc cgcgacaccg agagctatcc
     2221 ccagattacg ccgcatcgct cctccattac ggaagccggg catcaacggg cagaagccga
     2281 aatgcgctct gcgttcgtgc atccgattcg ccgccgtttc ggccatcact cccaatgtag
     2341 aggcctaccc ctcgggaaga tggagcaaac ggcacatcgg agaatcacga agcccccggg
     2401 caacgcccgg gggcttcgtg ggaagtggcg cttccgccgt caagcggaag ctcatatctc
     2461 gcgagcgtta tccgcccacg ccaccaccct ggacgtggcc ctgcaggtgc ggcgggatct
     2521 cgggcgcctc aggcacctcg acatcctcga cctgccaggt cgtccgggtt ccctgaacgg
     2581 tgccgaaggt tccgaagttc acgctcacgg tggccgtcac ctcgctggcc gagcggcccg
     2641 tcgtggttcc gggcggcacc tggacgacga agggcccgtt gggcacttca ccggtgagcg
     2701 ccgcgtaagc gctgaggaga agcgtctcca ccggttcgct cacggtcggg aagtccagcg
     2761 actggctgat gcggccgatc gagacgtagc cgttgaagcc agcgatcggc gggaacacga
     2821 cgttctcgcc cctctgcacc ggcgccggca gcgtccaggt ggccgggacg atcagggcgt
     2881 aggtgcccgt cgactcaccg atggcgaagg tgacctgagt ggaggtcacg ttgacgatcg
     2941 caccgggaac cagctcccat gcgccacgct gctcgttgaa gaagaccacg gccagcgcgt
     3001 ccgcaggaat ctggcgcgag ctgaggcccg tgacgatcag gcccgcggga agcggaaccg
     3061 taaccgtcgg cttggggccg gtgaagctca ccgaagcgtt cagagcgcga atgtcgatac
     3121 ccgcgag
//
LOCUS       DAOSCV010000528         3122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20020_length_3122_cov_3.309097, whole genome shotgun sequence.
ACCESSION   DAOSCV010000528 DAOSCV010000000
VERSION     DAOSCV010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20020_length_3122_cov_3.309097"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLY56_12660"
     CDS             complement(<1..336)
                     /locus_tag="PLY56_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ22375.1"
                     /translation="MGRSVVLSPMGYLEQVMQAAREVNPAEVDALAGAIEEAYHGGRF
                     VFIIGNGGSGANASHLCEDLGKGTLTDFRGQKRLRVLSLTDNTPYILAWGNDTSFERV
                     FVEQLRNLAG"
     gene            complement(512..1141)
                     /gene="thiE"
                     /locus_tag="PLY56_12665"
     CDS             complement(512..1141)
                     /gene="thiE"
                     /locus_tag="PLY56_12665"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOJ22376.1"
                     /translation="MIEPFGLYLILTDPVAGYECCAEAAVEAGVRFLQLRMKGVPRSD
                     VLAMARRLRAITRGTATRLIINDDPSVAAEAGADGAHLGQEDMPLAEARARWPQVPLW
                     GLSTHNEEQARAAVALAPDYIGVGPVYPTPTKAKPDPTVGLERLRKILAETPLPAVAI
                     GGIDAERLPEVARAGAENFAVVRAVCQAPDPLSAIHRLQTIWEEARVGR"
     gene            complement(1134..1916)
                     /locus_tag="PLY56_12670"
     CDS             complement(1134..1916)
                     /locus_tag="PLY56_12670"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOJ22377.1"
                     /translation="MNATEERITGAIVRSYFEKLQQHLAIDVAIVGAGPSGLVAARDL
                     ARAGLRVALFESKLAPGGGIWGGGMLFNEIVVQADALPILDDFGIRHRPADAPGLHTA
                     DAVEVASGLIFGALQAGATLFNAIRVEDIVFHEDRIAGVVIQWTPIARLQMHVDPLVV
                     MARAVVDGTGHPSEIVAMATRKAGVRIDTPTGDILGERPMWVDQGEASTVEQTRRLYP
                     GLYACGMAANNVGGGFRMGPIFGGMLKSGRKLARLIVEDLRD"
     regulatory      complement(2012..2107)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2375..>3122
                     /locus_tag="PLY56_12675"
     CDS             2375..>3122
                     /locus_tag="PLY56_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ22378.1"
                     /translation="MTRVIFAYDLENAELCVQAAPVLARLHEEHQVPATFFCLGRVLE
                     QKGAQLKPILGRSPLFDLQSHTYAHRMLRDNAMHGPGVSLDVLREEITLGKEWVERVF
                     ERPCIGTRSGCGFFQGMRGAPDRLAIFAECGVRYISTYLRGPADSIPSGLQQAHWYTE
                     DGFPNLLELPGHGWHDNVLKGPRSSMLCLSWPPVLSWGIPNRPVRDPEEELAVQRVWY
                     ERAFSLGLDYISLVYHPHSVYRQSPDCQIID"
BASE COUNT          507 a         1063 c         1016 g          536 t
ORIGIN      
        1 cccggccagg ttgcggagct gctcgacgaa aacgcgctcg aagctggtat cgttgcccca
       61 ggcgaggatg tagggggtgt tgtcggtgag gctgagtacc ctcaggcgct tctggccccg
      121 gaagtcggtg agcgttcctt tccccaggtc ctcgcacagg tgcgaggcat tcgcgccgct
      181 cccgccgttg ccgatgatga agacgaaacg cccgccatgg taggcctcct cgatcgctcc
      241 cgcgagggcg tctacctcgg cgggattcac ctcgcgtgcg gcctgcatca cctgctccag
      301 atagcccatc ggactcaaga ccacgcttct gcccatctcg cttctcccgg tgggttggcc
      361 cttctcggct gatggccaac tcacgctcgc tagtcgcaac cgcgtgaact cttgccggat
      421 gatacacgac ccgcctgctg gcgtcaagag ggcagggggg atggggcatc cacgcccgtc
      481 gtggatgccc cttacggaac tgcgccactc gctagcggcc cacgcgtgct tcttcccaga
      541 tcgtctgaag gcggtggatg gccgagaggg gatcgggcgc ctggcacact gcgcggacca
      601 cggcaaagtt ctcggcaccg gcgcgcgcca cctccgggag gcgctccgcg tcgatccctc
      661 caatcgccac ggctggcagc ggcgtctcgg cgaggatctt ccgcaggcgc tccagcccaa
      721 cagttggatc cggtttcgcc ttcgtcggcg tgggatacac gggaccgacg ccgatgtagt
      781 cgggcgctag ggcaaccgcc gcgcgcgcct gctcctcgtt atgcgtggag aggccccaca
      841 gtgggacctg gggccagcgc gcgcgcgcct ctgccagggg catatcttcc tgcccgaggt
      901 gcgcgccatc ggcgccggct tcggcggcca ccgacgggtc gtcgttgatg atgaggcggg
      961 tggcggttcc cctcgtgatc gcgcggaggc ggcgcgccat cgcgagcaca tcggagcggg
     1021 gcacgccttt catccggagc tggagaaacc gcacccccgc ctcgaccgcg gcctcggcgc
     1081 agcactcgta gccggccacc gggtcggtga ggatgaggta gaggccaaac ggctcaatca
     1141 cggaggtcct ccacgatcag gcgcgcgagc ttgcgcccgc tcttcagcat cccgccgaag
     1201 atcgggccca tgcgaaagcc tccgccgacg ttgttcgcgg ccatgccaca ggcgtagagg
     1261 cccggataga ggcgccgggt ctgctccacg gtcgaggctt cgccctggtc cacccacatc
     1321 gggcgctcgc cgaggatatc gccggtgggc gtgtcgatgc gcacgcccgc tttgcgcgtc
     1381 gccatggcga cgatttcgct cggatggccg gtgccatcga ccacggcgcg cgccatcacg
     1441 acaagcgggt cgacgtgcat ctggaggcgc gcgatcggcg tccactggat gacgacgccg
     1501 gcgatccggt cctcgtggaa gacgatatcc tccacgcgga tggcgttgaa gagcgtggca
     1561 cccgcctgga gagcgccgaa gatgaggccc gaggcgacct ccacggcgtc ggcggtgtgc
     1621 aggcctgggg cgtccgccgg ccggtggcga atcccgaagt catcgagaat ggggagggca
     1681 tccgcctgga ccacgatctc gttgaacagc atgcccccac cccaaatccc gccgcccgga
     1741 gcgagcttgc tctcaaagag ggcgacgcgc aggccggcgc gcgccaggtc gcgcgctgcc
     1801 accaggccag acggtccggc acccacgatc gccacatcga tggctaggtg ctgctggagc
     1861 ttctcgaagt aactgcggac aattgcgccg gtaattcgct cttcggttgc gttcatgcca
     1921 gattcctttc gtgcgagcag gctagaggcg cgtggcccgt ggcgggtgaa aggagaagag
     1981 gcagaacgct agcagctccc gtggaaacgg gagcgctggg atgtcagttc ccttcgccgg
     2041 cattacccgg atcaggttca tgggtctctt ctcagccgcg ccgggcgcag caccccaact
     2101 gacgtttccc aagttacctg gccagcgcca ggcgtgtggg ttttgcccga gtgtatcaca
     2161 tccaggagac agtgtcaacc ggccccagtg gactaagtca acataatgag cattatcgga
     2221 agttagatag gcacgcctgg atgggctgcg cgcatccgtg ctggatggcg ccgcaacgac
     2281 gtctgcggcg ttgggaggga atccgcgccc aggagcgaac gcttctccaa ccccgctcgc
     2341 gatctcgcgg gcgcgatgtg aggagaaagc cgccatgacg cgtgtcatct ttgcctacga
     2401 cctggagaat gccgagctgt gcgtgcaggc cgcgcccgtg ctcgcgcgcc tgcacgagga
     2461 gcaccaggtc ccggccacct ttttctgcct gggccgcgtt ctcgaacaga aaggcgccca
     2521 gctgaagccc atcctcggac gctcgccact cttcgacctc cagtcgcaca cctacgcgca
     2581 ccgcatgttg agagacaacg cgatgcacgg cccgggcgtc tcgctcgacg tgctgcgcga
     2641 ggagatcact ttaggcaagg agtgggtcga acgcgtcttc gagcgcccct gcatcggaac
     2701 gcgctcggga tgcggtttct tccagggaat gcgcggcgcc cccgaccgtc ttgcgatctt
     2761 cgccgaatgc ggggttcgct acatcagcac ctacctccgc gggcccgccg actcgatccc
     2821 ctcggggctg cagcaggccc actggtacac cgaagatggc ttcccgaatc tcctggagct
     2881 tccagggcac ggctggcacg ataacgtcct gaaaggtccg cggtcctcca tgctctgcct
     2941 ctcgtggccg ccagtgctct cctggggcat tccaaatcgg cccgtgcgcg atcccgagga
     3001 ggagctcgcc gtccagcgcg tctggtacga gcgtgccttc tccctcggcc tcgattacat
     3061 ctcgctcgtc tatcaccccc actcggtcta ccgccagagc ccggattgcc aaattattga
     3121 tc
//
LOCUS       DAOSCV010000529         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20027_length_3121_cov_3.422701, whole genome shotgun sequence.
ACCESSION   DAOSCV010000529 DAOSCV010000000
VERSION     DAOSCV010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20027_length_3121_cov_3.422701"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="nusB"
                     /locus_tag="PLY56_12680"
     CDS             complement(<1..453)
                     /gene="nusB"
                     /locus_tag="PLY56_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ22379.1"
                     /translation="MAANRRRQIRELAFRLLFQVDVGKLPMADVAASFREAVPRDRTD
                     TRLEEAAIRLAEMAVEHLGEVDEMIQGAAEKWPLERLAAVDRNIMRLAVTEMLYGHDV
                     PPSVAINEAVELAKKYGTEDSGRFVNGVLGAVARTHCASEESTTGEKPE"
     gene            complement(458..1801)
                     /gene="accC"
                     /locus_tag="PLY56_12685"
     CDS             complement(458..1801)
                     /gene="accC"
                     /locus_tag="PLY56_12685"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOJ22380.1"
                     /translation="MLKKILVYPRGEIAVRIIRACRELRIATVAVYSEADADSLHVRM
                     ADEAVRIGPAAAQDSYLKMQNVLSAALITGADAIHPGYGFLAEHATFSEACAACNIKF
                     IGPSAKSIEAMGDKAHARALMIEAGVPVVPGSEGVLNDEQRALRTAAQIGYPLMIKAA
                     AGGGGRGIRVVHSEDELVRELRTAQQEADAAFGDGSLYLEKYIEEPRHVEFQILADED
                     GNTIHLGERDCSLQRRHQKIVEEAPCIALTDSLRERMGAAAVAAAKAARYTNAGTVEF
                     LLDKHGNFFFLEMNTRIQVEHPVTEVITGIDLVKAQIAIASGLPLPWTQDRIRVRGHA
                     IECRINAEDPDRQFAPSVGEVKSLLLPGGPGVRVDTHLYPGYRLPSHYDSLLAKLICW
                     GQDRNEAVARMERALDEFVLDGMHTTIPFHRRIMSNAYFRRGEVYTNFIQRRMET"
     gene            complement(1879..2310)
                     /locus_tag="PLY56_12690"
     CDS             complement(1879..2310)
                     /locus_tag="PLY56_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22381.1"
                     /translation="MIRDVMRDESGMSALSLVGLLVCLGILVALAAVLIPSYTGAGGG
                     SATGAGGGSATATAPPGKAATPIQRAESVECQTNLTQIRQAITMYHTNNERVPANLEE
                     LGTFGVSSSMRHCPVGGAGYPYGYDPRSGRASCRYPGHERY"
     gene            complement(2364..2783)
                     /locus_tag="PLY56_12695"
     CDS             complement(2364..2783)
                     /locus_tag="PLY56_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ22382.1"
                     /translation="MDLTQVAELVAAVCSSRTVTEMTLREGTRRLVVRRAFAVEAPQP
                     EVVEAPTVDEYVVRSPLVGLFHQSASAKCPPVTVGMAVRDGQILGAIESMSVLYDVTA
                     EVSGVITEVLVEEGQPVEYGQELFRIQRAPDGESAPA"
     gene            complement(2787..2936)
                     /locus_tag="PLY56_12700"
     CDS             complement(2787..2936)
                     /locus_tag="PLY56_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22383.1"
                     /translation="MAFGQVNTGVLHETFTNPLVKWLIFACVGVYALTYVLRFWEYLF
                     FKLKI"
BASE COUNT          573 a         1087 c          895 g          566 t
ORIGIN      
        1 ctcgggtttc tctcccgtgg tcgactcctc gctggcacag tgggtgcgcg cgaccgcgcc
       61 caacacgccg ttgacgaagc gtccggagtc ttcggtgcca tactttttgg ccagctccac
      121 cgcctcgttg atcgcgacgc tgggcggcac atcgtgcccg tagagcatct ctgtcacggc
      181 aaggcgcatg atgttgcgat ccacggccgc caggcgctcg agcggccatt tctcggcagc
      241 gccctgaatc atctcatcga cttcccccag gtgctccacg gccatctcag ccagccggat
      301 cgccgcctct tccaggcggg tatcggtgcg gtcgcggggc acggcctctc gaaacgaagc
      361 cgcaacgtcc gccatcggca gcttgcctac atccacctga aagaggagcc tgaaggccag
      421 ttcccggatc tgccgccgtc tatttgccgc caccctctca cgtctccatt cgtcgttgga
      481 tgaaattggt gtagacctcg ccgcgcctga agtaggcatt cgacatgatc cggcgatgga
      541 acggaatggt ggtgtgcatc ccatccaaca cgaactcatc cagcgcgcgc tccatgcgcg
      601 ccacggcctc attccggtcc tgcccccagc agatgagctt cgcgagcaaa gagtcgtagt
      661 gcgagggcag ccggtagcca ggatagagat gcgtgtcgac ccggaccccc ggaccacctg
      721 gcaagaggag cgatttcact tcgcccaccg aaggcgcaaa ctgccggtcg ggatcctccg
      781 cgttgatccg gcactcgatg gcatggcctc tcacccggat tctgtcttgc gtccaaggca
      841 acggcaggcc cgatgctatc gcgatctgtg ccttcaccag atcgatgccg gtgatcacct
      901 ccgtcaccgg gtgttccacc tggatacggg tattcatctc caggaagaag aagtttccgt
      961 gcttgtcgag aaggaactcg accgtgcccg cattggtata gcgcgctgcc ttcgccgcgg
     1021 ccacggcggc ggcccccatg cgctcgcgca gcgagtcggt gagcgcgatg cagggcgctt
     1081 cctcgacgat cttctgatgc cggcgctgca gcgagcaatc gcgctcgccc aggtggatcg
     1141 tgttcccatc ctcatccgcg aggatttgga actccacgtg ccgcggctcc tcgatgtact
     1201 tctccaggta gagcgagccg tcgccaaacg cggcatccgc ctcctgctgg gcagtgcgca
     1261 attcgcgtac cagctcatcc tcggagtgca cgacgcggat gccccggcca ccgcctccgg
     1321 ccgccgcctt gatcatcagc ggatagccga tctgcgccgc agtccgcagt gcccgctgct
     1381 cgtcattcag cacgccctcg gaccccggca ccacgggtac cccggcctcg atcatcaacg
     1441 ctcgggcatg cgccttatcg cccatggcct cgatgctctt cgccgaggga ccgatgaact
     1501 tgatgttgca cgcagcgcaa gcctcggaga aggtagcgtg ctccgcgagg aagccatacc
     1561 cggggtggat cgcatcggcg cccgtaatca gcgcggcgct cagcacgttc tgcatcttca
     1621 gatacgagtc ttgggccgca gccgggccga tgcgaaccgc ctcatccgcc atgcgcacat
     1681 gtagcgagtc ggcatccgcc tccgaataga cggcgacggt cgcgatccgt agctcgcggc
     1741 acgcgcggat aatccgcacg gcgatctccc cgcgtgggta aaccagtatc ttcttcagca
     1801 ttcctgtaaa gcctatctga gattcacttc ctttagaatc cggcgcccat cctccgggaa
     1861 gacacgccct cgtagccttt agtaacgctc gtgtcccggg tagcgacagc tcgcacgccc
     1921 actccgagga tcatacccat atgggtagcc ggcgccaccc accgggcagt gccgcatcga
     1981 agatgaaacg ccgaaggttc ccaactcctc aaggttggca ggcacccgct cattgttcgt
     2041 atggtacatc gtgatcgcct ggcgaatctg agtcaggttg gtctggcact cgacgctctc
     2101 cgcgcgctgg attggcgtgg ccgccttccc tgggggcgcg gtcgcggtcg cagagccacc
     2161 cccagcgccg gtcgcagaac cgcccccagc gccggtgtag gaggggatga gaacggctgc
     2221 cagggcaacc agaatcccca ggcaaaccag gagacccacc aggctgaggg cgctcatccc
     2281 gctctcgtcg cgcatcacgt cacggatcat ccgacacctc ccgctcactc gccaccgggg
     2341 cgccgccagc ggcatccatc ggctcacgcg ggcgcgctct ctccatccgg cgcccgctgg
     2401 atgcggaaga gctcctgacc atactccacg ggctggccct cttccaccag cacctcggtg
     2461 atcaccccag agacctctgc ggtgacatcg tagagcacgc tcatcgactc gatagcgccg
     2521 aggatctggc catcccgcac cgccatcccc acggtgaccg gcgggcactt ggccgacgcg
     2581 ctctgatgaa agaggcccac gagtggagag cggaccacat attcgtcaac cgtcggggct
     2641 tccaccacct ccggctgggg cgcctcgacg gcaaaggcgc gtcgaaccac caaccggcgc
     2701 gttccctcgc gcaacgtcat ctctgtcacc gtgcggctgc tgcacacagc ggcgaccaac
     2761 tcagcgactt gtgtcaaatc cattgcttag atctttagct tgaaaaagag atactcccag
     2821 aagcgcagga cgtaggtgag cgcatacacg ccaacacagg cgaagatcag ccacttcacc
     2881 agtggattcg tgaacgtctc gtggagcact ccggtattga cctgtccaaa ggccactgcc
     2941 tgaacgccgc gtgccgccca ttgtcagaag ctcgcatacg tctgctcaat cgtttcctgg
     3001 aagaaccaga acgcgcccac caccaggggt accgacagcg gcgccacata gcggtgcttg
     3061 atcctctggt gctccaggaa gatgaagcag ttcttacact ttagacgttt gccgatcgta
     3121 c
//
LOCUS       DAOSCV010000530         3114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20086_length_3114_cov_3.531219, whole genome shotgun sequence.
ACCESSION   DAOSCV010000530 DAOSCV010000000
VERSION     DAOSCV010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20086_length_3114_cov_3.531219"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLY56_12705"
     CDS             complement(<1..967)
                     /locus_tag="PLY56_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOJ22384.1"
                     /translation="MGELPHLPLPHPLARQRAFHVMAKPIGPRCNLRCAYCFYLEKDH
                     LYPGERSFRMSDETLESFIRQYIEAQEVPEVSFAWQGGEPTLLGIEFFRRVVELQRKY
                     LPPGMRLINALQTNGTLLDAEWCRFLRENNFLVGISIDGPRELHDHFRQDAKGGPSFE
                     HTLRGLRLMREHGVEFNVLCVVNRVNSQHPLECYRFFKEEGVRFIQFIPIVERLAEEA
                     PCLGAAPSTRDVSEASVLPEEYGRFLCEIYDEWVRNDVGKVYIQTFEVAFSAWLGHRA
                     GLCAFEPTCGTALALEHNGDLYSCDHYVYPEYRLGNLMEQPLVALA"
     gene            complement(960..1877)
                     /locus_tag="PLY56_12710"
     CDS             complement(960..1877)
                     /locus_tag="PLY56_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HOJ22385.1"
                     /translation="MEKIVSISLGSPSRDFTVEVDLLGRRLAVQRRGVGGSYHDYFRA
                     LAEADADPEVRAIGLGGINRYLLTNANKHEFSMARKMVATVRTKPVVDGCGLKTSLEP
                     YLVHRLQEEGVVDFSRSRVLMVAATDRWGMARALRETACETVFGDLMFGLDLPIPLGW
                     NVLCPLAALLVPVITRCVPFKWLYPTGETKVKPKYGNWYAWADVVAGDWKFIQRCLPV
                     GPEALRGKTILTNTVTSKDVELLRSRGAALLVTTTPNLSGRSFGTNVIEAVVITLAGK
                     RPEEMTPQEYLDVLRALGWDRPRVEHLNG"
     gene            complement(2171..2431)
                     /locus_tag="PLY56_12715"
     CDS             complement(2171..2431)
                     /locus_tag="PLY56_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22386.1"
                     /translation="MATVDAVEVMEEIVTEEPMRNHNHDLIQVLSEKLDGVVRYPVYH
                     EDAVDECEVCMRIWEQIKQDDMRHVEMLRDEIIRHAKEGTFD"
     gene            complement(2668..>3114)
                     /locus_tag="PLY56_12720"
     CDS             complement(2668..>3114)
                     /locus_tag="PLY56_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22387.1"
                     /translation="VPKAPPARALKAPDPNEIAPTSTAEWWKECASEKELRQEAGTSV
                     SLPSRLPPGYALVAYYLRGCRRGGTLPVVRYDDGLNALTLFITPGPGGRRRIGRGWGW
                     GRDRNASALSQVAGQQVARVAHEGYTYILVGDHDPDGLEATLRSIR"
BASE COUNT          537 a         1004 c         1037 g          536 t
ORIGIN      
        1 cggccagcgc taccagcggc tgctccatca ggttgcccag gcggtactcg ggatagacgt
       61 agtggtcgca cgagtagaga tcgccgttgt gctccagggc gagcgccgtg ccacacgttg
      121 gctcgaaggc gcacagcccg gcgcgatggc ccagccaggc gctgaaagcg acctcaaacg
      181 tctggatgta gacctttccc acgtcgttac gcacccactc gtcgtagatc tcgcacagga
      241 aacggccgta ctcttccggg agaaccgagg cttcggatac gtcacgcgtg gatggtgccg
      301 ccccgaggca cggggcctct tccgccaggc gctccacaat cgggatgaac tggatgaacc
      361 ggacgccctc ctctttgaag aagcggtagc attccagagg atgctggctg ttgacgcggt
      421 tgacgacgca gaggacgttg aactccacgc cgtgctcgcg catcaggcgg aggccccgga
      481 gggtgtgctc aaatgacgga ccgcccttcg cgtcctggcg aaagtggtcg tgaagctcgc
      541 gcgggccgtc gatgctgatg cccaccagga agttgttctc gcgcaggaag cggcaccact
      601 ccgcgtcgag gagcgtgccg ttggtctgta gcgcgttgat caggcgcatt cccggaggca
      661 ggtacttccg ctgcagctcg acgacgcggc ggaagaactc gatgccgagc agcgtaggct
      721 cgccaccctg ccaggcgaag cttacctcgg gcacttcctg cgcctcgatg tattggcgga
      781 tgaagctctc caacgtctca tccgacattc ggaaggagcg ctcgcccggg tagaggtggt
      841 ctttctccag atagaagcag taggcgcagc gcaggttgca ccgggggccg atgggcttcg
      901 ccatcacgtg aaacgcacgc tgccgggcca gggggtgggg caacggcaga tggggaagct
      961 cacccattga gatgctccac gcggggccgg tcccagccca gcgcgcgcaa tacatccagg
     1021 tactcctggg gtgtcatctc ttcggggcgc ttcccggcca gggtgatgac gacggcctca
     1081 atcacgttgg ttccgaagga ccgcccggag aggtttgggg tggtcgtcac aagcagcgca
     1141 gcgccacgcg agcggaggag ctccacatcc ttgctcgtca cggtgttcgt caggatcgtc
     1201 ttgcctcgca gcgcttcggg gcccacggga aggcagcgct ggatgaactt ccaatcgccg
     1261 gcgaccacat cggcccatgc ataccaattg ccgtacttcg gcttcacttt ggtctcaccg
     1321 gtggggtaga gccacttgaa gggcacacag cgggtgatca ccggcacgag cagcgcggcg
     1381 agcgggcaga gcacgttcca gccgagcggg atcgggagat cgagcccgaa catcaggtcg
     1441 ccgaagaccg tctcgcaggc ggtctcgcga agcgctcgcg ccatgcccca gcggtccgtg
     1501 gccgcgacca tgagcacccg gcttcgcgag aaatcgacca cgccctcctc ctggaggcgg
     1561 tggacgaggt agggctccag gctagtcttc aggccgcagc catccacgac cggcttggtg
     1621 cgaacggtgg cgaccatctt gcgcgccatg ctgaactcgt gcttattcgc gttggtgagg
     1681 aggtagcgat tgatcccccc gaggccgatg gcgcgcacct ccggatcggc atccgcctcg
     1741 gcgagggcac ggaaatagtc gtgataggag ccacccactc cgcggcgttg gaccgcgagg
     1801 cgtctcccga ggagatccac ttcaacggtg aagtcgcgcg agggggagcc cagggatata
     1861 ctgacgatct tttccataca actccagggc tatcagagtg caagtgcagg caacaagcgt
     1921 cggggagatc cccgaggggc gacggtggac gcgcacgacg ggcgcggaac cggtgttcac
     1981 ccgcgcgata cacgaggggg ccgcgctagc actccgaatt ccttatctcg gaatcccaga
     2041 tacgctcccg agggaagatt tctcctcaat caggcgcgtt cctgcaagag cgagagcggc
     2101 cggaagtgga aacccgacgc gcgggggcgg catggcaccg tcgcgcgccg ggattgaaac
     2161 cgtcagcgtc tcagtcgaag gtaccctctt tggcgtgccg gatgatctcg tcacgaagca
     2221 tctcgacgtg cctcatgtca tcctgcttga tctgctccca gatgcgcatg catacctcgc
     2281 actcgtctac cgcgtcctca tggtagacgg gataccggac cacgccatcc agcttttcgc
     2341 tcagcacctg gatcaggtcg tggttgtggt tgcgcatcgg ctcttccgtg acgatctctt
     2401 ccatgacttc aacggcatct accgtggcca ttctcgtcct cccctggggg gcgatgcccc
     2461 gtggtggagt gccggcggca tcccgtctgg agttggggcg cgcactgcgc tcgccgcgtt
     2521 cggcgggatc ggcccttcat gggcgtgccc tctctcgcca gcaccctcgc cgggttcatt
     2581 cccggcgtct cggagaggta aacgcagccg cggtgccggc cgcgagccgg gcgcagagac
     2641 cggatgatct cccacattgg ccgcgctcta ccggatggag cgcagcgttg cctccagccc
     2701 atctggatcg tgatcgccaa ccaggatgta ggtatagccc tcgtgcgcga cgcgcgccac
     2761 ctgctggccg gccacctggc taagcgcgct tgcgttccgg tcccgccccc agccccatcc
     2821 ccggccgatg cggcgccggc caccgggccc gggcgtgatg aaaagcgtga gcgcgtttag
     2881 gccatcgtcg tagcggacaa cggggagcgt tccaccacgg cggcacccgc gcaggtagta
     2941 ggcgacgagc gcgtagcctg gagggagccg gctgggcagc gaaacggagg ttccggcctc
     3001 ctggcgcagc tccttctccg aggcgcattc cttccaccac tccgcggtgg aggtcggggc
     3061 gatctcattc gggtcggggg ctttgagggc gcgggcgggc ggtgctttgg gcac
//
LOCUS       DAOSCV010000531         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20127_length_3110_cov_4.119804, whole genome shotgun sequence.
ACCESSION   DAOSCV010000531 DAOSCV010000000
VERSION     DAOSCV010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20127_length_3110_cov_4.119804"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLY56_12725"
     CDS             <1..952
                     /locus_tag="PLY56_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22388.1"
                     /translation="AADNNSVTRLLRREQVVAENRQVRMINPTRTNLAFQRHWFHLRV
                     EKEGARLRGYVDGKLACDYTDPQPLPGGQVALWTSRNGLMVARARVWAEKPGRRNPLA
                     ALPVAPAPPSSASWSLGATSPPALACDFERGLGGWTAEGGANGPVLSLDRESRAQGKQ
                     SLRVTNPISGGSLGALCGIRQFDAVKFPILEFDYRMDPRARVNLYLRVGRQAMVLGLS
                     AAQLAEPSIPSLGAVEGFVADGKWHRARIDLLGALRRFYPTATTILVEDLAVRSPEVE
                     YLRSGFGGNTWGVSYHLDNFRLLGAPETKPEDAKAVVVRE"
     gene            complement(981..1736)
                     /gene="pgl"
                     /locus_tag="PLY56_12730"
     CDS             complement(981..1736)
                     /gene="pgl"
                     /locus_tag="PLY56_12730"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOJ22389.1"
                     /translation="MEPRDVRLYPDAEAVAEAAADYLIALAGDALAHRDRFCLALSGG
                     STPRMLYARLARPEEAGRVDWSRVHLFWGDERCVPPDHPESNYRMAWDVWLRHAAIPA
                     ENIHRVQGELPPPDAAADYERELRAFFVPESLPRFDLVLLGMGEDGHTASLFPESPAL
                     HEHHQWVMAVETRQSVPWRVTLTPPAINAAAHVLFLVTGAAKAERLQEVLHGPHEPER
                     LPAQIIQPTSGSLTWMVDAAAGSLLYFTSDTLP"
     gene            complement(1785..>3110)
                     /gene="zwf"
                     /locus_tag="PLY56_12735"
     CDS             complement(1785..>3110)
                     /gene="zwf"
                     /locus_tag="PLY56_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOJ22390.1"
                     /translation="FSRRPYADQHFRDLLRESAAHTIGNSFDGAAWERFASHIHYVPG
                     DALVHEDYARLRAFVRQGENGPANRLYYLATPPSLYAPTVERLGAVGMAREDDGWRRI
                     IVEKPFGHDLASAQQLNRDLHAVFDEHQIYRIDHYLGKETAQNILFFRFANLIFEPVW
                     DRRYVSSVQITVAESVGVGHRARYYDQAGVLRDMFQNHLLQLLALVAMEPPASFNADA
                     VRNEKAKVLGAIRPVLPSDTVRAQYRGYCNTPDVAPDSQTATYAALKLYVDTWRWQGV
                     PFFLRSGKALAEKSSEVSLEFQCPPRLLLDDASTCSLRSNVLTICIQPDEGIHLKFEM
                     KEPGSSSELRSVDMDFHYRSILRGRRLPDAYERLLLDALNGDASLFTRADEIELAWRL
                     IDPIQQAWEGPDAPPLEIYEAGSWGPEGANALIRRSGRVWRRGCCDHLE"
BASE COUNT          498 a         1029 c         1102 g          481 t
ORIGIN      
        1 cgcggcggac aacaacagcg tgacccggct cctccgtcgg gagcaggtgg tcgcggagaa
       61 caggcaggtg cgcatgatca acccgacgcg caccaacctg gcgttccagc ggcattggtt
      121 ccacctgcgc gtcgagaagg agggggcgcg cctgcggggc tacgtcgatg ggaagctggc
      181 gtgcgactac accgacccgc agccgctgcc cggcggccag gtggcgctct ggacctcgcg
      241 caacggcctg atggtggcgc gtgcccgggt ctgggctgag aagccgggcc ggcgcaaccc
      301 gctggccgcc ctgccggtgg cccccgcgcc gccgtccagt gcctcttgga gcctgggtgc
      361 gacctcaccg cccgcgctcg cgtgcgattt cgagcgcggc ctgggcggct ggaccgccga
      421 aggcggcgcg aacggccccg ttctctcgct cgaccgggag agccgggcgc aggggaagca
      481 gagcctgcgc gtgaccaacc cgatctctgg cggcagcctg ggcgccctgt gcggcatccg
      541 ccagttcgac gcggtgaagt tcccgattct ggagttcgac tatcggatgg acccgcgagc
      601 gcgcgtcaac ctctacctcc gggtgggccg ccaggcgatg gtgctcgggc tgtcggcggc
      661 gcagctggcg gaaccgtcga tcccgtcgct tggcgccgtc gagggctttg tcgccgacgg
      721 aaagtggcac cgggcgcgta tcgatctcct cggcgcactc cgccggttct atcccacggc
      781 caccacgatc ctcgtcgagg atctagcggt ccgcagcccc gaagtggagt acctgcgctc
      841 cggcttcggc ggaaacacct ggggcgtaag ctatcacctg gacaacttcc ggctgctggg
      901 cgccccggag acaaagccgg aagacgcgaa agcggtggtg gtgagggagt agccgcgcgc
      961 cggcctggcc ggcgcctgat tcacggcagc gtgtcgctcg tgaagtagag gagcgagcct
     1021 gcggccgcgt cgaccatcca ggtgaggctg ccggacgtcg gctggatgat ctgcgccggc
     1081 agccgctcgg gctcatgcgg gccgtggagc acctcttgca gccgctctgc cttcgcggcg
     1141 ccggtcacca ggaacagcac gtgcgccgcg gcgttgattg ccggcggcgt gagtgtcacc
     1201 cgccacggaa cggattgcct ggtctcgacg gccatgaccc actggtggtg ctcgtgtagc
     1261 gcgggactct ctgggaagag cgaggccgtg tgcccatcct ctcccatgcc caggaggacc
     1321 aggtcgaacc gggggaggga ttctggaacg aagaacgcgc gcaactcgcg ctcgtagtcg
     1381 gccgcggcgt ccgggggcgg cagctcgccc tggacgcgat ggatattctc cgccggaatc
     1441 gccgcgtggc gcagccagac gtcccaggcc atgcggtagt tgctctcggg gtgatccggg
     1501 ggcacgcacc gctcgtctcc ccagaaaagg tgaactcgcg accaatcgac acggccagcc
     1561 tcttccggcc gggccagccg ggcgtagagc atgcgcggcg tcgacccgcc ggagagtgcc
     1621 aggcaaaacc ggtcgcggtg ggccagggcg tcgccagcca gggcgatcag gtagtcagca
     1681 gccgcctccg cgaccgcctc cgcgtctgga tacaggcgaa catctcgcgg ttccatgcct
     1741 cctcctgcca atgagccgtt caaaggcgag cgggcgacgc ggtttcactc gaggtgatca
     1801 cagcagccac ggcgccagac gcgcccgctt cgccggatca gggcattcgc tccctccggc
     1861 ccccaactcc cagcctcgta gatctccagc ggcggggcat ctgggccctc ccacgcctgc
     1921 tggattgggt cgatgaggcg ccacgcgagc tcgatctcat ccgcgcgcgt gaagagggat
     1981 gcgtcgccat tcagcgcgtc gagcagcagg cgctcgtagg cgtcagggag gcgccggccg
     2041 cgcaggatgg agcggtagtg gaagtccata tccaccgagc gcagctcgct ggacgaaccc
     2101 ggctccttca tctcgaactt gaggtgaatc ccctcatccg gttggatgca gatcgtgagg
     2161 acgttggacc gcaggctgca ggtagacgcg tcgtcgagca ggagccgcgg agggcactga
     2221 aactccaggc tcacctcgct gctcttctcg gcgagcgcct ttccggagcg caggaagaac
     2281 ggcacgccct gccagcgcca ggtatccacg tacagcttga gggcggcgta ggtggcggtt
     2341 tgcgaatcgg gggcgacgtc gggcgtgttg cagtagccgc ggtactgggc gcgcacggtg
     2401 tccgatggca acaccggccg gatggcgccg agcacctttg ccttctcgtt gcgcacggca
     2461 tcggcgttga acgaggcagg cggctccatc gcgacgagcg cgaggagctg gagcaggtga
     2521 ttctggaaca tgtcgcggag gacgcccgct tgatcgtagt agcgcgcccg atgacctacg
     2581 cccacgctct ccgccacggt gatctgcacg ctgctcacat agcgccggtc ccagactggc
     2641 tcgaagatca ggttggcaaa ccggaagaag aggatgtttt gcgccgtctc cttgcccagg
     2701 tagtgatcga tccggtagat ctggtgctcg tcgaagacgg cgtgcaggtc gcggttcagc
     2761 tgttgcgccg aggcgaggtc gtgcccgaac ggcttctcca cgatgatccg gcgccacccg
     2821 tcgtcctcgc gcgccatgcc cacggcaccc agacgctcga ccgtgggagc atagagagaa
     2881 ggcggcgtcg ccaggtagta gagacgattt gccgggccat tctctccctg gcgcacgaag
     2941 gcccggaggc gggcgtaatc ctcatgcacc agggcatcgc cgggcacgta atggatgtga
     3001 gaggcgaacc gctcccaggc agcgccgtcg aagctgttgc cgatggtgtg cgccgccgac
     3061 tcgcgcagga gatcgcggaa gtgctggtcg gcatagggcc ggcgcgagaa
//
LOCUS       DAOSCV010000532         3110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20129_length_3110_cov_3.304746, whole genome shotgun sequence.
ACCESSION   DAOSCV010000532 DAOSCV010000000
VERSION     DAOSCV010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20129_length_3110_cov_3.304746"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLY56_12740"
     CDS             complement(<1..1203)
                     /locus_tag="PLY56_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOJ22391.1"
                     /translation="MAGTKREKLMVVDGNSLLYRAFFALPNLTTSDGQHTNAVYGFSM
                     MLLRLLDEEKPDYCVVAFDAPGPTFRHEAYAEYKATRQKTPDELRSQGPLARQVCQVL
                     SIPILEVPGVEADDVVGTVARYAERESIDVLIVTGDMDALQLVTERVHVLATRRGITD
                     TVRYDPEAVEERYGVRPELIPDLKALTGDPSDNIPGVRGIGMKTAVKLLQRYGSVEGL
                     LQARDEVPEPRIRQALQDAGEQPRESRVLATIVTDVELDAGLEAWRCQEPDRPKAREL
                     FDQLEFRSLRSRFDEDDTVTAAPVSAQPLATIADLDRLVSRACARRGVALALAPTPER
                     GGQAATGAQPELDLGQDARRLPELAGLGLGVDDGVYVWDPTKSEVTLAMLRPLLEDPR
                     VPKCAHDLK"
     assembly_gap    1226..1235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1236..2133)
                     /gene="sppA"
                     /locus_tag="PLY56_12745"
     CDS             complement(<1236..2133)
                     /gene="sppA"
                     /locus_tag="PLY56_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOJ22392.1"
                     /translation="MLAILFFAFTVIAGFGARSSPSLPVGEKVGLIYVEGPIVGGRGA
                     ASLFGISSGADVIVRQLDRARRDRSIKAVVLRINSPGGSAAASQEIYQEVMKLRKARK
                     PVVASMGDVAASGGYYVAAAADKIMANGSTMTGSIGVIMEFLNLQGLYEKVGLGTVTL
                     KSGRFKDIGSATRPLTEDERQLLQAMIDDVYDQFVSDIVAGRKVLSREDVRRLADGRI
                     FTGRQAKKLKLVDELGNLQDAIALAGKMAGMTGRPAVEEVGRRGFLDIFLGEDSGYGS
                     LMRPPMSGAEEERLLSALRHYMR"
     gene            2808..2894
                     /locus_tag="PLY56_12750"
     tRNA            2808..2894
                     /locus_tag="PLY56_12750"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2842..2844,aa:Leu,seq:caa)
BASE COUNT          517 a         1093 c          938 g          552 t
ORIGIN      
        1 cttcaggtcg tgtgcgcact tgggcacgcg cgggtcctcc aggagagggc ggagcatcgc
       61 aagcgtcacc tcgctcttcg tgggatccca tacgtagacg ccatcgtcca ctccgagtcc
      121 caagccggcc aactcgggga gccggcgcgc gtcctgcccc aggtccagtt ctggttgcgc
      181 tcctgtagcg gcctgcccgc cgcgctcagg ggtcggcgcg agtgcgagcg ccacgccacg
      241 ccgggcacac gcccgcgata ccagccgatc gagatccgcg atggttgcca gcggctgcgc
      301 ggagacaggc gcggccgtga cagtatcatc ctcatcgaac cggctgcgca ggctgcggaa
      361 ctcgagctga tcgaacagtt cgcgtgcctt cggacggtcg ggctcctgac accgccacgc
      421 ctccagaccc gcgtccagct ccacatcggt cacaatcgtc gccaggacgc gcgactctcg
      481 aggctgttcg ccggcatcct gcagtgcctg gcggattcgc ggctcgggca cctcgtcccg
      541 ggcctgaagg aggccctcca cagacccata gcgttgcagg agcttgacag cggttttcat
      601 cccgatcccg cgcacaccgg ggatgttatc ggaaggatcg cctgtcagcg ccttcaggtc
      661 gggaatcagc tccgggcgca cgccgtagcg ttcctcgacg gcctcggggt cgtagcgcac
      721 cgtatcggta atcccccggc gcgtggccag aacgtggacg cgctccgtca cgagctgtag
      781 cgcatccatg tcgccggtga cgatgagcac gtcgatgctc tcccgctcgg cgtagcgcgc
      841 cacggtgcca accacgtcat ctgcctcgac gccaggcacc tccaggatcg ggatgctgag
      901 tacctggcac acttggcgcg ccagtgggcc ctgggatcga agctcgtcgg gcgtcttctg
      961 gcgtgtggcc ttatactcgg catatgcctc gtgccggaaa gtggggccgg gggcatcgaa
     1021 cgcgaccacg cagtaatcag gcttctcctc atccagcagg cgtaggagca tcatgctgaa
     1081 gccgtacacc gcattcgtgt gctgcccatc tgaagtcgtc agattcggca acgcgaagaa
     1141 cgcccgatac agcaggctgt tcccatccac caccatcagc ttttcgcgct ttgttccggc
     1201 catgttccct cttgcttacc tcggcnnnnn nnnnngtcgc atgtaatggc ggagcgcgct
     1261 cagcaggcgc tcctcttcgg cgccgctcat gggcgggcgc atcagacttc cgtaacccga
     1321 gtcctccccc aggaagatgt cgaggaatcc gcgccggccc acctcttcca cggcaggccg
     1381 cccggtcatg ccggccatct tcccagcgag ggcgatggcg tcctggaggt ttccgagctc
     1441 atccaccagc ttcagcttct tggcctgccg gccggtgaag atccgcccat cggcaaggcg
     1501 gcgaacatcc tcgcggctca gcacctttcg cccggcaacg atgtccgaca cgaactggtc
     1561 atacacatca tcgatcatcg cctgcaagag ctggcgctca tcctcggtga gcgggcgcgt
     1621 ggcggagcca atatccttga accggccgct cttaagggtc accgtgccta ggcccacctt
     1681 ctcatagagg ccctgcaggt tcagaaactc catgatcacg ccgatgctcc cggtcatcgt
     1741 ggagccgttc gccatgatct tatcggctgc cgcggccacg tagtagccgc cagacgcggc
     1801 cacgtcgccc atcgaggcga ccaccggctt gcgcgccttt cgcagcttca tcacttcctg
     1861 atagatctcc tgcgaggccg cagccgatcc acccgggctg ttgatgcgca gcaccaccgc
     1921 cttgatactg cggtcgcggc gggcgcggtc caactggcgc actatgacgt cggccccgct
     1981 ggagatgcca aagaggctgg ccgctccccg cccgcccacg atggggccct ccacgtagat
     2041 cagccccacc ttctcgccga cgggcaaaga tggactggag cgcgccccga agcccgcgat
     2101 cacagtgaat gcgaaaaaga ggatcgccaa caccaggcag ccgccacagc caccagcggc
     2161 ccacggaagc catgccggac ggcacgggcg cggtggacgg cttgtcctgg tgtgggcagc
     2221 acctccctgc ccgctgcgcg gcggcacggc accgaaaccg gtttccggag cttgcgggcc
     2281 cgtgcccgca gaggtcattc gaatgtgcgg tgcgctctca ggcgaaacat cttttgcctc
     2341 cgcacccccc gcctcaagcg tgctgggccc ggcgcccctc tcttcaggga aggcggtctc
     2401 atcctctgcc tcttcaggcg gcccgcccgg ctccctcgcg ccctcggcgc tggctgtttc
     2461 ggaagcatcc cgtgcctcgg cggcatctcc atgctcaggg cgacgctcgg tcgtgctcgg
     2521 ctcgcccgcc tggtctcttt cccctgatgt gtctggcaag ctctgctctc tctcctgatg
     2581 gtctgacatg ggagtaccct ccgacgatct accgcggcag cgcggggcgg catgtttcaa
     2641 agcgcacggg caaagcaaca caatctgctg aggcgagggg agtggctgca gacatcgttt
     2701 cgtccacccg gcatgcttcc gagcaaccgc ttagacaaga ccggatgggc aaagcatcgg
     2761 agcgttgaca tgacgccctg ggcgtgctat aatcgcaatg ctgccatgcc gaagtggcgg
     2821 aataggtaga cgcggcggtc tcaaaaaccg ctgggggcaa cctcgtgccg gttcgagtcc
     2881 ggccttcggc accacccatt cacctcgcga tttcccccgt agtacactgg tcattatagc
     2941 atggcgccgc gcgcaccgtc aaacagaatg acgccctgcc tgggattccg agatgagaag
     3001 gctgcccgca cgcgcggagc tcgaagctcc gtgctgcttt ccggcaaagc ccctgcgggg
     3061 cttctcctcc cagctaccgc cgttttgaga catctgggag tatccccaac
//
LOCUS       DAOSCV010000533         3108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20143_length_3108_cov_3.550934, whole genome shotgun sequence.
ACCESSION   DAOSCV010000533 DAOSCV010000000
VERSION     DAOSCV010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20143_length_3108_cov_3.550934"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLY56_12755"
     CDS             complement(<1..458)
                     /locus_tag="PLY56_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ22393.1"
                     /translation="MAQPADELFERVAGARREWMACFDAITDWVFVKARDLSIMKANR
                     AAASIFGRIPQQLIGHTCQELFQTDEMPADEAVVLQTFQTGQPQSVDVDTMPVPGEFH
                     VATYPLRDESGHVVAVVEYVRDVTEQKAMQRQLLQSARLAAVGELAAGVA"
     gene            complement(475..2928)
                     /locus_tag="PLY56_12760"
     CDS             complement(475..2928)
                     /locus_tag="PLY56_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ22394.1"
                     /translation="MDPAVGLDSVNPSWAIWFRLLVEHANDLIYLHDPDGKLLFVNRR
                     LEQVTGWSRDEIIGRNLLEFLAPDYRPSALARREDAALPEGGRRYETEIVARDGTRIP
                     LEVHSTPVKHNGVTIAWQGIARDIRERRQAQARMNALYETARQVVGILHLDQVLKVIV
                     SHAAEITGALSCTVWMPDEQRETLYCSASGGTAGTAEGERIAINSDDPLAEAFRSRQA
                     TVRDLYDGSLAGLAALPADAALMLAFPLIAAEGVLGVLGLCYGRPSRPNPDEVALLSI
                     FANHAAAAIANARLFEAQRRKTQEAAAIGQVGMAIGTLDVEAALRVAGAYAYDLLSAD
                     LAAVLVLDPDRSTLRTAFIAERHRQVTDEQFQEVYRLDEHPLLAEAIQNDRTVVVDAA
                     AHPLMRDGDGARGEGMGTTLVVPMSSRSEVDGALVISYRERTHVSDVRIRLAETLAQQ
                     AAVAIDNAMLFRATEAAKREWEATFDAIRDPVFIFSTEGRLLRANRAAGGMLGQEVTS
                     LIGRHYLELLHGGSAPTPVDIFAECLAQGQPVTREVEDLAVPGAFQVSAYPLFDDTGK
                     CVGVVEYLKDVTAQREMQARLYHSARLAGVGELAAGVVHNFKNVLMGVSGMLDVALML
                     IERGGSMESVRERLVDAQGHVFRGNQVLQRLLDFARGMPQEVADVHLDVLLPDVVALC
                     KAHPASRGIRIRAELPANLPPVRADASQLHEVLVNLVLNALQAIVGGGTVTVDAACEG
                     DRVTIRVKDTGCGIPPEEIERIFEPFYSHRVHGPPGSGIGLSSSLRMVRAMGGDIRVE
                     SQVGVGTSFIVELPWGTGE"
BASE COUNT          551 a         1047 c          972 g          538 t
ORIGIN      
        1 tgcgccacgc ccgccgcgag ctctcccaca gccgccaggc gcgccgattg aagcagctgg
       61 cgctgcatcg ccttctgctc ggtgacgtct cggacatact ccaccaccgc caccacatgg
      121 cctgattcgt ctcgcagtgg gtaggtggcc acatggaact cgcccgggac cggcatcgtg
      181 tccacatcga cgctctgagg ttggcccgtt tggaaggtct gcagcaccac agcctcatcg
      241 gcgggcatct catccgtctg gaagagctcc tgacacgtgt gcccgatgag ctgctgcggg
      301 atccgcccga agatcgacgc ggcggcccgg ttcgccttca tgatggagag atcacgcgcc
      361 ttgacgaaca cccagtcggt gatcgcgtcg aagcacgcca tccactctct gcgcgcccct
      421 gctactcttt cgaacaactc atctgccggc tgcgccatac gcttgtcctc agtatcactc
      481 tccagtcccc cacggcaact ccacgataaa ggaggttccg actcccacct ggctctctac
      541 gcgaatgtcg ccccccatcg cgcgtaccat gcggaggctg gaggaaaggc ctatgcccga
      601 gcctggcggt ccatgtaccc ggtggctgta aaatggctcg aagatccgct ctatctcctc
      661 gggcgggatg ccgcacccgg tatccttgac gcggatggtc acccggtcgc cttcgcaagc
      721 cgcatcgacc gtcaccgtac ccccgccgac aatcgcctgc agcgcgttga gcaccagatt
      781 caccagcact tcgtgtagct gactggcgtc ggcacgcacc gggggcaagt tggccggaag
      841 ctcggcacga atgcggattc cacggctggc tggatgcgcc ttgcacagag ccactacatc
      901 cggcagcaga acatccaggt ggacgtcggc cacttcttga ggcattcccc gcgcgaaatc
      961 gagcaggcgt tgaagcacct ggttacctcg aaagacgtgg ccctgggcgt ctacgaggcg
     1021 ctcgcgcacc gattccatac taccgccgcg ctcgatcagc atcagggcga catccagcat
     1081 tcccgagacg cccatgagca cgttcttgaa gttgtggacg actcccgcgg cgagctcacc
     1141 tacgcccgcc agacgcgcgg agtgataaag acgcgcctgc atctcgcgct gagcggtaac
     1201 atctttaaga tattcgacaa cgccgacaca tttgcctgta tcatcaaaca gcgggtaggc
     1261 ggaaacctgg aaagcgcctg gcaccgcgag gtcctccacc tctcgggtca ccggttgacc
     1321 ctgcgccagg cactccgcga agatatcgac gggggttggc gcgctcccgc cgtgaagaag
     1381 ctccaggtag tgacgcccga tcaacgaggt gacctcctgg cccaacatgc cgccagctgc
     1441 ccgattggcc ctcaagaggc ggccctcggt ggagaagata aagacagggt cgcggatggc
     1501 gtcgaaggtc gcctcccact cgcgctttgc cgcctcggtc gcccggaaga gcatggcgtt
     1561 gtcgatggcg accgcggctt gctgcgcgag cgtttcggcc aggcggatgc gaacatccga
     1621 gacgtgcgtg cgctctcgat agctgatgac gagagcgcca tccacctcgc tccgcgagct
     1681 catcggcacg accagggtgg ttcccatgcc ctcgccacgc gcgccgtcgc catcgcgcat
     1741 cagcgggtgt gctgccgcgt ccacgaccac cgtgcgatcg ttctggatag cctcagccag
     1801 aagcggatgc tcatccaacc ggtagacttc ctgaaactgc tcatcggtca cttgccggtg
     1861 gcgctcggca ataaacgcgg tgcgcagcgt gctccggtcc ggatcgagca ccagcacggc
     1921 cgccaggtcg gcggagagga ggtcataggc ataggcgccg gcaacgcgca gagcggcctc
     1981 gacatccagg gtgccgatgg ccatgcccac ctgcccgatc gccgcggcct cctgggtctt
     2041 tcggcgctgc gcctcgaaga ggcgcgcgtt ggcgatggcg gcagcagcgt ggttcgcgaa
     2101 gatcgagagg agcgccacct cgtccgggtt ggggcgcgag gggcgaccgt aacacagccc
     2161 gaggacgccg agaactccct ccgccgcgat cagcggaaag gccaacatca atgcggcatc
     2221 cgcgggaagg gcggcaaggc ccgccagcga tccgtcatag aggtcgcgga cggtcgcctg
     2281 acgcgaccgg aacgcttcgg ccagcggatc atccgagttg atggcaatcc gctctccctc
     2341 cgcggttcca gccgtgcccc cggaagcaga gcaatagagc gtctcccgct gttcatccgg
     2401 catccaaacg gtgcagctta gcgcgcccgt gatctccgcg gcatgcgaga cgatgacctt
     2461 gagcacctgg tccaggtgga ggattcccac cacctgccgc gccgtctcgt agagagcatt
     2521 catgcgcgcc tgcgcctggc gccgctcgcg gatgtcgcgc gcgatgcctt gccaggcaat
     2581 cgttacgccg ttatgtttga ccggggtaga gtggacttcg aggggaatgc gggtaccgtc
     2641 ccgagcgacg atttcggtct cgtagcggcg gccgccttcg ggcagcgccg cgtcctcgcg
     2701 ccgtgccagc gcgcttggcc ggtagtccgg tgccagaaac tccaggagat tgcgcccgat
     2761 gatctcgtcc cgtgaccatc cggtgacctg ctccaggcgc cggttgacga agagcagctt
     2821 tccgtcaggg tcatggaggt agatgaggtc gttcgcgtgc tcaacgagga ggcggaacca
     2881 gatggcccac gaggggttga ccgagtccaa tccgacagct ggatccaaga cgtaacgccc
     2941 ccggttctcc cgtggtttga cacgaggcgc gtctggattg gattttcgtg ggcttcccag
     3001 ttccattagt tgtattccgg ggggagagac cggcccctga ttgacgcgcc ggcgcgatga
     3061 gacctatctc atcggtgagg cgcgtccgcc tccgccgtgc cggcggcg
//
LOCUS       DAOSCV010000534         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20230_length_3099_cov_2.165243, whole genome shotgun sequence.
ACCESSION   DAOSCV010000534 DAOSCV010000000
VERSION     DAOSCV010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20230_length_3099_cov_2.165243"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2843
                     /locus_tag="PLY56_12765"
     CDS             <1..2843
                     /locus_tag="PLY56_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22395.1"
                     /translation="IAPDRAYLPARDPRRSGCLYTPDSDYDALALYDGAAADKGSATS
                     GLGVRIPWGTPEPAASVPPEKPAIPYTRKYVWWSFGGDDPTNPDPNRWEDATGTAFLS
                     GSLRKARPDAPSPSILQGRWHTGASWTESGYVGPRVGVITPFGQPVGDYSNQFVLYED
                     RNDNGILDAGDYPALKAFQVKVTVTDRQLTEEPTAYSIDRPVVGSVGNADPTLDYAGT
                     FVAWAIPHPSDTSKTRYIFSSDSQPAAARFWDPSTANHRRLALFWTSNRRDDLFGGGG
                     RQPQPPAASVPAYERSQSWYLYGTRSYEIKDHPEKYWPFWTNRSPHYDLSDAASRLAS
                     ASPPELKAPGISLFDGQLANQGTLLSERYTAPNIVQTVAASGQTGQPYYLFFQGTGQA
                     RDAGGRTYRASQIFYTTLGSDFSVNSATPIIPVGLEQPEVVKHTPRGLSYVDASGVHR
                     LWAFWHGGAENAWQGYFSLNRGNKALATDWKLEDGRGFGILPLYGDFATVQDLVPLYR
                     PGPHTNLLTGATTSAPMIDLLFTGFSKSRGNADVYMARLDPAQLAWGTRGSWRAKQPF
                     APVEKELLVARSNRVYISRHLEWMTSPLYRTTASGRRFVFPRIWWRPAGSTTLQLITP
                     DPENASHAPSNRRWATGTWRYDQEGTRWIYDWKDAPTAYGVGNTITIYPLAGQVVFAK
                     PLNPGDEIYVDYVPTILRVAGSPQMDASPAAFVDTRVGPAQSPVDGTRIYRQRDRLWL
                     FWTRIAPQVSKPTVWYKTMRLMVQLPRAIQPGTEVTVTDEFGAAITQFTIDTGVHPTT
                     GQILYPGRLYFPASQTAGVPRGLEGRRIIVSYTEAGTGAAGPQPVDPYIRWHDERPET
                     PLPIDDVANEGSLCAIPDLPLADPDSDSEYLSDAGAWRVWLFWTSSRPKATVYDASTG
                     QPRQFSNDTDLRYMTIAPRLDFRPTLPGATSP"
     gene            complement(2871..>3099)
                     /locus_tag="PLY56_12770"
     CDS             complement(2871..>3099)
                     /locus_tag="PLY56_12770"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOJ22396.1"
                     /translation="EAMGHYLWHEGHHAVTAELKVSFHSPAYVWQRLRVSGRIREVRG
                     RLILCTARAERDDGTLVASAEAKFVRVVDRA"
BASE COUNT          528 a         1073 c          997 g          501 t
ORIGIN      
        1 gcattgctcc ggaccgcgcc tacctcccgg cgcgcgaccc ccggcgcagt gggtgtctct
       61 acacgccgga ctcggattac gacgccctcg ctctctacga cggtgcagcc gccgataagg
      121 gctcagcgac gagtggcctg ggagtccgca ttccgtgggg cacccccgag cccgccgcca
      181 gcgttccccc ggagaagccc gcgataccct acacccggaa gtacgtctgg tggtctttcg
      241 gaggcgatga tccgacaaac cccgatccga accgctggga ggatgccacc ggcaccgcct
      301 tcctctcggg ctcgcttcgc aaagcaaggc cggatgcgcc ctcgcccagc attcttcagg
      361 gccgatggca cacgggcgcc tcgtggaccg agtcgggcta tgtcgggccg cgtgtgggcg
      421 tgatcacccc cttcggccag cccgtgggtg attactcgaa ccagttcgtc ctctacgagg
      481 accgcaacga taacggcatc ctggacgcgg gcgactaccc cgcgctcaag gcgtttcaag
      541 tgaaggtgac cgtcaccgac cgccagctca ccgaggagcc gacggcctat agcattgacc
      601 ggcccgtggt gggctcggtt gggaatgccg acccgacgct cgactatgcc ggcaccttcg
      661 ttgcgtgggc gatcccgcac ccctcggata cgagcaagac gcgctacatc ttcagtagtg
      721 attcccagcc ggccgccgcg cgcttctggg atccgagcac ggcgaaccac cgccgcctgg
      781 cgctcttctg gacgagcaat cgccgggatg atctcttcgg cggcggaggg cgccagccgc
      841 agccgccggc cgcgtccgtg cccgcgtacg agcggtctca gtcgtggtac ctctacggca
      901 cgcgctccta cgagatcaag gaccatccgg agaagtattg gcccttctgg accaaccgct
      961 ccccgcacta cgacctctcc gacgcggcgt cgcggctcgc ctcggcgtcg ccaccggagt
     1021 tgaaggcgcc tggcatctcg ctcttcgacg ggcagctggc caaccaaggc accctcctct
     1081 cggagcgcta caccgcgccg aacattgtgc agacggtggc agccagtggc cagaccggcc
     1141 agccgtacta cctcttcttc cagggcacgg ggcaggcgag agatgctgga gggcgcacct
     1201 accgtgcctc gcagatcttc tataccactc tggggtccga tttcagcgtg aacagcgcga
     1261 cgcccatcat cccggtcggc ctggagcagc cggaggtggt gaaacacact ccacgcgggc
     1321 tgagctacgt ggatgccagc ggcgtgcatc ggctgtgggc cttctggcat ggcggggcgg
     1381 agaacgcctg gcaaggctac ttcagcctga accggggcaa caaggcgctg gccaccgact
     1441 ggaagctcga ggatggacgc ggcttcggca tcctgcccct ctatggtgat ttcgccaccg
     1501 tgcaggacct cgtacctctc tatcgcccgg gcccgcacac caacctccta acgggcgcga
     1561 ccacctccgc accgatgatc gatctcctct ttacgggctt ctcaaagtcg cgcgggaacg
     1621 cggacgtcta catggcccgt ctagatccag cgcagctggc atggggcacg cgcggatcat
     1681 ggcgcgccaa gcaacccttc gcacccgtcg agaaggagct gctggtcgcc cggagcaatc
     1741 gcgtttacat ctcgcggcat ctggaatgga tgacctcgcc gctctaccgg accaccgcga
     1801 gcgggcgccg cttcgtcttc ccgcggatct ggtggcggcc cgcgggcagc acgaccctgc
     1861 agctgatcac gcccgatccg gagaacgcct cgcacgcgcc ttccaatcgg cgttgggcga
     1921 cagggacctg gcgctacgac caggaaggca cccggtggat ctacgactgg aaagacgccc
     1981 ccacggccta tggcgtgggc aacaccatca ccatctaccc gctcgccggc caggtggtct
     2041 ttgccaagcc gctgaacccg ggcgatgaga tctacgtgga ttacgtgccc acgatcttgc
     2101 gcgtggccgg ctcgccgcag atggatgcgt cacccgcggc gtttgtggat acgcgcgttg
     2161 gcccggcgca gagcccggtg gatggcacgc gcatctaccg ccagcgcgat cgcctgtggc
     2221 tcttctggac tcgtatcgcg ccccaggtct cgaagccgac ggtgtggtac aagacgatgc
     2281 gcctgatggt ccaattgccg cgggctatcc agccgggcac ggaggtcacc gtcaccgacg
     2341 agttcggggc ggcgatcacg cagttcacta tcgacaccgg ggtacacccg acgacggggc
     2401 agatcctcta cccaggacgg ctctacttcc cggcaagcca aacggctggg gtgccgcgtg
     2461 gtctggaggg tcggcgaatc atcgtgagct ataccgaggc tggcacgggg gcagcgggtc
     2521 cccagccggt ggatccttat atccgctggc atgacgagcg cccggaaaca cccctgccga
     2581 tcgatgatgt agcgaatgag ggcagcctgt gcgcgatccc ggatctgccg ctggcggatc
     2641 ctgattcgga cagcgagtac ctgagtgacg ccggcgcatg gcgcgtgtgg ctcttctgga
     2701 cgtcatcgcg accgaaggcg accgtctatg acgcctctac ggggcagccg cggcagttct
     2761 ccaacgacac ggacctgcgc tatatgacga tcgcgccgcg gctggacttc cggccgacgc
     2821 tgccgggggc cacctcgcca tagcgggagg gggagtcagg ggaatatggg ctaggcgcga
     2881 tcgacgacgc ggacaaactt cgcctcggca gaggcgacca acgtgccgtc gtcgcgctca
     2941 gcccgggccg tgcataggat cagccggccg cgcacctcgc ggatgcgccc ggagacacgc
     3001 agccgctgcc agacgtaggc cggactatgg aacgacacct tcagttcggc ggtgacggcg
     3061 tgatggccct cgtgccagag gtagtgcccc atcgcttca
//
LOCUS       DAOSCV010000535         3096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20246_length_3096_cov_3.782966, whole genome shotgun sequence.
ACCESSION   DAOSCV010000535 DAOSCV010000000
VERSION     DAOSCV010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20246_length_3096_cov_3.782966"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2205
                     /locus_tag="PLY56_12775"
     CDS             <1..2205
                     /locus_tag="PLY56_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22397.1"
                     /translation="RRVIAEISVSAERAEALPPLACEQAGVFATVNDDPDLITTWYEG
                     FLLYRLVGAVPLPERPAPRLLPQEEARAGWERLAGRAKPAAVDRRRLAEANQLLDVSP
                     EPPDMLHGNPNHYFDPRTNSILRFHAPGKHWSEKEKKYIDLSELPEQVQRAGAYAHHC
                     YLSSGALKLAEVGWQTGDRRYFRKAAEILLAYARHYPRYAYARPAGVAFRSRVGWAVL
                     QESWWYRPLPRALDLVRASGVLTAEEDRAIVEGVILPAAIHLRTHRSVANQQAEYNSG
                     VGIGALVAGHWPLAAEALHGEYGLRAQWKRDFDADGWSVERDTSYHFAALKPFVEMAE
                     AYENAGVHVFDEEFKRLFDAPVLQSPDLKSPGFTDGYVTAYERYRDPLYLRTVAVARR
                     QPVSPPSGGFTNSILHASGLTVLRAGADDASLRTVSVNWGSPAHRGGKVMLDPKATWK
                     GFPLNERVFRIAYGYKQSGFSYTAAAGNSLVVDGKPSSMLRVDQVAVLDGPMPAGRWT
                     SPLHRPLYPGVGWSRTAAFCGDTFVLVDQLASERPRCFDLFAFLPHDVTGTVPAQTVW
                     KPFPEFAGQGHGYDSFEEPEVAGEAPPTGFDYRLDAKDERPRGRLTLLSPEGTRLFRM
                     KGYIRWHPVRVPVIVRRLENARNAWAVAAFTGRMEENAPLPAIRVLPVEREGRELPPH
                     EALAIEVTDASGKYLLLTSEHAGAHRVAGCALEGPLATLALEGPSRASRAGQ"
     gene            complement(2298..>3096)
                     /locus_tag="PLY56_12780"
     CDS             complement(2298..>3096)
                     /locus_tag="PLY56_12780"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOJ22398.1"
                     /translation="HPALTDVPVGAWTAAAALDAIAAARNSEAVDQCATGAIAIGLVG
                     ALGAAAAGLMDWRHISHQQPRRLGLTHGLLNIGAALLYGTSLIARRRGARGVGRGLAF
                     LGYATAGLSAYLGGHLVFEHHLGVDHATEQKPPQEWTWVMAESDLPVGEPRRVDVGDA
                     RILLLRRGDQILAIGEKCTHLGGPLAEGKLEGDSIVCPWHGSRFSLEDGHVINGPAVF
                     PERCIETRVRDGQIEVRAGGRGESCQHHRAQPAPVESEAREMLMTGS"
BASE COUNT          465 a         1058 c         1054 g          519 t
ORIGIN      
        1 cgccgcgtga tcgccgagat cagcgtgtcg gccgagcgcg cggaggccct cccgccactc
       61 gcctgcgagc aggcgggcgt cttcgcgacg gtcaacgatg atcccgatct catcaccacg
      121 tggtacgaag ggtttctgct gtatcgcctg gtgggagcgg tgccgctgcc ggaacgcccg
      181 gccccgcgcc tgcttccgca agaggaagct cgcgccgggt gggagcgact ggcgggccgg
      241 gcaaagcccg cggcggttga tcggcgccgg cttgccgagg cgaaccagct gttggatgtc
      301 tctccggagc cgcccgatat gctccacggc aacccgaacc actactttga cccgcgcacc
      361 aactccatcc tgcgcttcca tgccccgggc aagcactggt ccgagaagga gaagaagtat
      421 atcgatcttt ccgagctgcc ggagcaggtg cagcgtgcgg gcgcctatgc gcaccattgc
      481 tacctctctt ccggggcgct caagctggcg gaggtgggat ggcagacggg tgaccggcgc
      541 tacttccgca aagcggcgga gatcctcctc gcctacgcgc gccactaccc ccgctatgcc
      601 tacgcacggc ccgctggcgt tgccttccgc tccagggtgg gatgggccgt tctccaggag
      661 agctggtggt accgcccgct accccgcgcg ctcgatctgg tgcgtgcctc gggggtcctc
      721 accgccgagg aggatcgtgc catcgtggag ggtgtgatcc tacctgcggc gatccacctg
      781 cgcactcacc ggagcgtggc caaccagcag gcggagtaca acagcggcgt cgggattggg
      841 gcactggtgg ccgggcattg gccgctggct gccgaggcgc tgcacggcga atacggcctg
      901 cgcgcgcaat ggaagcgcga ctttgatgcc gatgggtgga gcgtcgaacg agacaccagt
      961 taccatttcg cggcgctgaa gccgttcgtg gagatggcgg aggcctacga gaacgcgggc
     1021 gttcacgtct tcgacgagga gttcaagcgc ctcttcgacg ctcctgtgct ccaaagcccc
     1081 gatctgaagt cgcccgggtt cacggatggc tacgtcaccg cctacgagcg gtatcgtgac
     1141 ccgctctacc tgcgcacggt ggcggtagcg cggcgccagc ccgtgtcccc gccgtccggc
     1201 ggcttcacca actccatcct acacgcatcg gggctcaccg tgctccgcgc cggcgctgac
     1261 gacgcgtcgt tgcgcacggt gagcgtgaac tggggcagcc ccgcgcatcg cggcggcaaa
     1321 gtgatgctcg atccgaaggc gacctggaag ggcttcccgc tcaacgagcg cgtttttcgt
     1381 atcgcctacg gctacaagca atccgggttc tcttacaccg ccgccgcggg taactcgctg
     1441 gtggtcgatg ggaagccttc gtccatgctc cgcgtggatc aggtggcggt tctggatgga
     1501 ccgatgcctg ccggacgctg gaccagcccg ctccaccgcc cgctctatcc aggagtgggc
     1561 tggtcgcgga ccgcggcgtt ctgcggcgat accttcgtgc tggtcgatca gcttgcctcg
     1621 gagcgcccgc gctgcttcga tctctttgcc ttcctcccgc atgatgtcac cggcaccgtt
     1681 ccggcgcaga ccgtgtggaa gccgtttccc gagttcgccg gccagggaca tgggtatgac
     1741 agcttcgagg agcccgaagt ggcgggcgaa gctcccccaa ccggctttga ctaccggctg
     1801 gacgccaagg atgagaggcc gcgcggccgg cttacgctgt tgagccctga gggaacgcgc
     1861 ctgtttcgca tgaaggggta tatccgctgg cacccggtcc gcgttccagt gatcgtccgg
     1921 cgcctggaga atgcgcgcaa cgcgtgggct gtggcggcat tcaccggtcg gatggaagag
     1981 aacgcccccc tgcccgcgat ccgcgttctt ccagtcgagc gcgaggggcg cgagctgcca
     2041 ccccacgagg cgctcgccat cgaggtgacc gatgcctcgg gaaagtacct cctcctgacg
     2101 agcgagcatg caggcgccca ccgcgttgct ggatgcgccc tcgaggggcc gctcgccacg
     2161 ctcgccctgg aaggcccctc gcgtgcatcg cgcgctggcc agtaatctgg gaagctggcg
     2221 ccagtgcggt ggcaggcggc gctccaaatc ggcgcgccgc ccgaagctcg gtggcatccc
     2281 ctgggcgtga cgcccgctca ggaacccgtc atcagcatct cgcgtgcttc gctctctacc
     2341 ggggccggct gcgcgcggtg gtgctggcag ctctcgccgc gcccgcccgc gcgtacctca
     2401 atctgcccat cgcgcacacg cgtctcgatg caccgctccg ggaagacagc ggggccgttg
     2461 atcacgtgcc cgtcctccag cgagaaccgg gatccatgcc aggggcagac gatgctatcg
     2521 ccctccagct tgccctcggc gagtggcccg cccagatgcg tgcatttctc accaatggcg
     2581 aggatctgat cgccgcgtcg gaggagcagg atgcgcgcgt caccaacgtc gacgcggcgt
     2641 ggctctccca ctggcaggtc cgattcggcc atcacccagg tccattcttg aggcggcttc
     2701 tgctccgtgg cgtggtccac cccgagatgg tgctcgaaga cgaggtgccc gccgaggtag
     2761 gcggagaggc cggcggtggc atagccaagg aaggcgaggc cgcgtccaac cccgcgggcg
     2821 ccgcgcctcc tggcgatgag tgacgtgcca tagaggaggg cggcgccgat gttgaggaga
     2881 ccatgcgtca acccaagccg gcgcggttgc tggtggctga tatggcgcca gtccatcagt
     2941 ccggctgccg ccgcgccgag cgctccgacg agcccgatgg cgattgctcc ggtggcgcac
     3001 tggtccactg cttccgagtt gcgtgcggcc gcgatggcat ccagggcggc cgctgccgtc
     3061 caggcgccca cgggtacatc tgtcagcgcg gggtgg
//
LOCUS       DAOSCV010000536         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20294_length_3092_cov_3.378005, whole genome shotgun sequence.
ACCESSION   DAOSCV010000536 DAOSCV010000000
VERSION     DAOSCV010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20294_length_3092_cov_3.378005"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..732
                     /locus_tag="PLY56_12785"
     CDS             163..732
                     /locus_tag="PLY56_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOJ22399.1"
                     /translation="MDFPTLLLIALGLAMDASAVALSVGFALRRLTFRPVFRLSFHFG
                     LFQALMPIIGWAAGKSVHAYIQAYDHWVAFILLAYIGGRMIQAGLSDQEEEAIVKDPT
                     RGARLVMLSVATSIDAMAVGLLLALMGVSIWYPSLVIGIVAALCTLLALFVGHCCGAA
                     LGRRMEVVGGSVLIAIGFRILYQHIYLAA"
     gene            complement(866..1354)
                     /locus_tag="PLY56_12790"
     CDS             complement(866..1354)
                     /locus_tag="PLY56_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOJ22400.1"
                     /translation="MELKIVRIEKPENINFILGQSHFIKTVEDLHEALVGAMPGIQFG
                     IAFCEASGPALVRWSGTSDEMIALAKKNAEALSAGHSFIIFLGNAFPVNVLNAIKAVP
                     EVCRIFCATANPTEVIVAETEQGRGILGVIDGVKTKGVETDADIKERKELLRRFGYKL
                     SA"
     gene            complement(1387..2568)
                     /locus_tag="PLY56_12795"
     CDS             complement(1387..2568)
                     /locus_tag="PLY56_12795"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOJ22401.1"
                     /translation="MKYLLIVPDGMADDPVPELGGRTPLMAAATPHLDAIAAEGMVGR
                     VHTIPEGMPPGSDVAALSIMGYDPARVYTGRAPLEAASQGIALGPDDVAFRCNLITVD
                     DGRVVDHSGGEISSEEAAQLIEAIQERLGSDRVRFYPGVSYRNLMVWHGGTTGMETTP
                     PHDILGQAMEPHLPRGEGASLLLQLMRESRDVLADHPVNRKRKAEGKSPANLIWLWGQ
                     GRAPRLESFPRLRGVSGAVISAVDLIRGIARLAELNVVLVPGATGRLDTDWAGKRDAA
                     RQSLAEVDFVMVHLEAPDECGHKGDLEGKIRAIEWTDREIIGPLWEAARSMGSARMLV
                     LPDHYTPLSLRTHVGKPVPFALAGEGIPRDAAERMDEATGEASPVCVSEGWRLIERLF
                     A"
     gene            complement(2702..>3092)
                     /locus_tag="PLY56_12800"
     CDS             complement(2702..>3092)
                     /locus_tag="PLY56_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22402.1"
                     /translation="DPYHGAVDSSEFVEDFYAWAALQVAKEREEEPEVTAEVQVFRIG
                     DAAICFNPAELFVAFGLRMKEASPFQPTFVVELANACVGYVPTPEAFTQGGYEPRTAP
                     SSKLVPEAGDQITEVTLKLLRQLDARR"
BASE COUNT          498 a         1085 c          930 g          579 t
ORIGIN      
        1 ccggtttcgc gtccatctcc cggcgcacag cggtggatca caccgggtag agcctctacg
       61 gtgcgccaag catcccccag atccactcaa cggaatctgc ttcataggag aatcgcgcct
      121 atcggcgaac cctattctgg agaggagcag gcagatagct acgtggattt ccctaccctt
      181 ctgttgattg cccttggtct tgcgatggat gcttcggcgg ttgccctcag tgttggtttc
      241 gcgctccgac ggctcacctt ccgacctgta tttcgactat cgtttcattt cgggcttttc
      301 caggcactca tgccgattat tggctgggcc gccggcaagt cggtccacgc ctatatccag
      361 gcctatgacc actgggtggc gttcatcctc ctcgcctaca tcggcgggcg catgattcag
      421 gcgggcctga gcgatcaaga ggaggaggcg atagtgaagg atccgacgcg aggcgcccgc
      481 ctggtgatgc tctcggtcgc gacgagcatc gacgcgatgg cagtggggct cctcctcgcg
      541 ctgatgggcg tctcgatctg gtatcccagc ctggtgatcg gcatcgtcgc ggcactctgc
      601 acgttgctcg cgcttttcgt ggggcactgc tgcggcgccg cgcttggccg gcgcatggag
      661 gtggtcgggg gcagcgttct tatcgcgatc ggcttccgaa tcctctacca gcacatctat
      721 ctcgccgcgt aacgcggggg cacagcgtgg gatgaggacc aggcgcgccc catcccatcg
      781 ctccatcccc ggcaagagtt gctcctgaac tcctgccctg gtggcctcgg acagcgtgct
      841 cgcaagaggt tcgcctccgc ggtcactacg ccgatagctt atagccgaag cggcgtagca
      901 gctccttgcg ctctttgatg tcggcgtcag tctccacgcc ctttgtcttc acgccgtcga
      961 tcacgccgag aatgccgcgg ccttgctccg tctcggccac gatcacctcg gtggggttgg
     1021 cggtggcgca gaagatgcgg cacacttccg gcacggcttt gatggcgttg agcacgttca
     1081 cagggaaggc gttccccagg aagatgatga acgagtggcc ggcggaaagc gcctcggcgt
     1141 tcttcttcgc cagggcgatc atctcgtcgc tcgtgcccga ccagcggacc aacgcgggtc
     1201 cggaggcctc gcaaaacgcg atcccgaact gaatgccggg catcgccccc acgagcgcct
     1261 catgcaggtc ctccaccgtc ttgatgaagt gtgactgtcc caggatgaag ttgatattct
     1321 ccggcttctc gatgcggacg atcttcagct ccattccctc tctcccttca cctcagcgcg
     1381 cgtttgtcac gcgaagagcc gctcgatgag tcgccagccc tcgcttacac agacgggcga
     1441 ggcctcgccg gtggcctcat ccatccgctc ggcggcgtcg cgggggatcc cctcgcctgc
     1501 caatgcgaag ggaaccggct tccccacgtg cgtgcgcagc gacagcggcg tgtagtggtc
     1561 gggcagcacc agcatgcgtg ccgaccccat ggagcgggcg gcctcccaga gcggcccgat
     1621 gatctcgcga tccgtccact cgatggcgcg gatcttcccc tccaggtcgc ccttgtgccc
     1681 gcactcgtcg ggagcctcca ggtgcaccat aacaaaatcg acttcggcga gcgattggcg
     1741 ggccgcatcg cgcttgccag cccaatcggt atctaggcgt ccggtggctc cgggcaccag
     1801 caccacgttc agctctgcca gccgggcgat ccctcgaatc agatccactg ccgagatcac
     1861 cgcgccagag acgccccgaa gcctcgggaa actctccaaa cgcggggccc gcccctgccc
     1921 ccaaagccag atgaggttgg ccgggctctt cccctcagcc ttgcgcttcc ggttcacggg
     1981 gtgatccgcc agcacatccc gcgattcgcg catcagctgg agcagcagcg acgcgccttc
     2041 cccgcgcggg agatgcggct ccatcgcctg acccagaatg tcgtgcgggg gagtcgtctc
     2101 catcccggta gttcccccgt gccagaccat caggttccgg tagctcacgc cgggatagaa
     2161 acgcacgcgg tcgctgccca gccgctcctg gatcgcctcg atcagctgcg ccgcctcttc
     2221 cgaggagatc tcgcccccgc tgtggtccac cacccgacca tcgtccaccg taatcaggtt
     2281 gcaccggaaa gcaacatcgt cggggcccag cgcgattccc tggctcgccg cttcgagggg
     2341 ggcgcggccg gtgtagacgc gcgccggatc atagcccata attgaaagcg ccgccacgtc
     2401 gcttcccggc ggcatcccct ccgggatggt gtgcacccgg cccaccatcc cctcggcggc
     2461 aatcgcatcc aggtgtggcg tcgccgccgc cattaggggc gtgcgcccgc ccagctccgg
     2521 caccggatca tccgccatcc catctggcac aatcaaaaga tacttcatct cccccgcgtc
     2581 ttctctcctc catcactcgc cgggccgcgg ctccgcccgt caaaacttcg cgctctcctc
     2641 cgggcgagcc cggccacccc tcacgaggga gggcacgccg ggcccgctgg aaagggatgc
     2701 gttagcgtcg tgcatccaac tgccggagga gcttcaaggt gacctcggtg atctggtcgc
     2761 ctgcctccgg aacgagcttg ctgctcggtg cggtgcgcgg ctcgtaacct ccctgcgtga
     2821 acgcctctgg ggtcggcacg tagccgacgc aggcgttcgc cagctccacc acgaaggtgg
     2881 gctggaaggg cgaggcttcc ttcatgcgca ggccaaaggc aacgaagagc tccgcagggt
     2941 tgaagcagat cgccgcgtcc ccaatgcgga agacctgcac ctcggcggtc acttccggct
     3001 cttcttcgcg ctccttcgcc acctggagcg ccgcccaagc gtagaagtcc tcgacgaatt
     3061 cggagctatc caccgcgcca tgataaggat cg
//
LOCUS       DAOSCV010000537         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20352_length_3086_cov_3.132629, whole genome shotgun sequence.
ACCESSION   DAOSCV010000537 DAOSCV010000000
VERSION     DAOSCV010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20352_length_3086_cov_3.132629"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..568)
                     /locus_tag="PLY56_12805"
     CDS             complement(296..568)
                     /locus_tag="PLY56_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ22403.1"
                     /translation="MAEEIKRKVSIRTCTGCGQERPRHELLRIVRTPEGQIVPDRTGK
                     LPGRGAYLCPKRACVEQAIKRKRLAKALRNAVPEEVMMQVEQWLDG"
     gene            complement(607..1704)
                     /gene="nusA"
                     /locus_tag="PLY56_12810"
     CDS             complement(607..1704)
                     /gene="nusA"
                     /locus_tag="PLY56_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOJ22404.1"
                     /translation="MVTAEFIHALRQIEKEKEIPLDKLLPTIEDALVSAYKKDYGGTG
                     AVRVVIDQTRPSVMVFAERLVVEHVEEPANEISLEQARKQNPQAQVGDVIEVEVTPDN
                     FGRIAAQTAKQVVLQRIREAERDAVFDEYQNRIGEVMTGIVQWREGRNVFVNLGKVEA
                     LLPPKEQVSGEPYRFGDRIRVLILDVRKAAKAPMVIVSRTHPNLLRRLFEMEVPEISD
                     GIVEIKSVAREAGARSKIAVASNDEKVDPVGACVGPRGSRVQSVVQELYNEKIDVVRW
                     SDDIREYVRAALSPAKPSSVQITDPERKSILVIVPSSQLSLAIGREGQNVRLAARLVG
                     WKIDIRDEARLAAEQAGAQQVAPETGGPEDE"
     gene            complement(1928..2338)
                     /locus_tag="PLY56_12815"
     CDS             complement(1928..2338)
                     /locus_tag="PLY56_12815"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22405.1"
                     /translation="MAARQRYHFFRDMGDATVESLLLSLADRLAARGPWATDAQVERH
                     RRFVEEMLRLSLEGATVAHPVVPIGGRELAEAVGCPQGPLLGDLLDALKEAVAVGEVS
                     TRGEAILFARGWLSSRRKRQESEAPPARGQSDAV"
     gene            complement(2375..>3086)
                     /locus_tag="PLY56_12820"
     CDS             complement(2375..>3086)
                     /locus_tag="PLY56_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ22406.1"
                     /translation="MALPVPFDAGNLEQSLVDPCGGLADLHARRLRHTHPDIFTADPV
                     RLLRAVRIAAALEFTLDPETEALVRRDAALLPQSAVERIRDELFALLEVPGSATWVRR
                     METLGLLAPILPETVALKGLTQNANHHLDVWEHTLEAMRQCEALLIGPELPEDLRAAV
                     AEQLRQPICAPRSRLALLLFSVLLHDIAKPQTRGETPEGIITFARHDVVGAEMAGAIC
                     RRYRLSKRETKRVVTAVG"
BASE COUNT          514 a         1067 c          943 g          562 t
ORIGIN      
        1 gttgagaact atcttgcgca gtgcatttgc ggttgcatca tccagggtga ctgcctggta
       61 gttgcggagg tgaatgccca tccccgaaag cgcggagact acctccttgg gctgcacatt
      121 caattccttt gccagatcga ctactcgtgt ttgagccaac tgaaaaccac cccccagttt
      181 ctgcttcact catggcgggt ggctggatgg tcaggatttc ataggcgaaa ccctccttgt
      241 acattgcacc gaccgatgcc ctgccgctcc ttggcgcggg gccacccagt cacgcctaac
      301 catcgagcca ctgctccacc tgcatcataa cctcttccgg gaccgcgttc cgcaatgctt
      361 tcgccaaccg cttcctcttg atcgcctgct cgacgcacgc tcgcttggga caaaggtaag
      421 cgccacgtcc gggcagcttg cccgttcgat ccggcacgat ctggccctcc ggcgtgcgca
      481 cgatccggag gagttcgtgg cgcgggcgtt cctgcccgca ccccgtacac gtccggatcg
      541 ataccttgcg tttgatctcc tccgccatgg cttctcacgc gcccggcacg aacgccgcgc
      601 ccgtggctac tcgtcctccg gcccgccggt ctccggggct acctgttgag cgcctgcctg
      661 ctccgcggcc aggcgagcct cgtcacggat gtcgatcttc caccccacca gccgggcagc
      721 gaggcgaaca ttctgaccct cacggccaat ggcgagcgaa agctgcgagc ttggcacgat
      781 caccaggatc gacttgcgct cggggtccgt gatctgcacc gaggacggct tcgccgggct
      841 gagcgcggcg cggacgtact cccgaatgtc gtcgctccag cgcacgacgt ctatcttctc
      901 gttatagagc tcctgaacca cgctctggac gcgcgaccca cgcgggccca cgcacgcccc
      961 gaccggatcc accttctcat cgttggaggc cacggcgatc ttcgagcgcg cgcctgcctc
     1021 gcgtgccacc gacttgatct ccacgatccc gtcggagatc tcgggcactt ccatctcgaa
     1081 tagccggcgc aggaggttcg ggtgggtgcg cgagacgatc accatcggcg ccttcgccgc
     1141 cttgcgcacg tccaggatca ggacgcggat ccggtcgcca aaccgatacg gctcgccact
     1201 gacctgttcc ttgggaggaa gcagcgcctc caccttgccc aggttgacga atacgttgcg
     1261 accctcgcgc cactggacga tgccggtcat cacctcgccg atgcggttct gatactcgtc
     1321 gaaaacagcg tcgcgctccg cctcgcggat ccgctggagc accacctgct tcgcggtctg
     1381 cgccgcgatc cgcccgaagt tatccggggt gacctcgacc tcgatgacat cgcctacctg
     1441 ggcctgcggg ttctgcttgc gtgcctgctc gagtgagatc tcgttggcgg gctcctcgac
     1501 gtgctcgacg accaggcgct ccgcgaagac catcacgctc ggccgggttt ggtcgatcac
     1561 cacgcgaacc gctcctgtgc ccccgtaatc cttcttgtac gcggagacga gggcatcctc
     1621 gatcgtaggc aggagcttgt cgagggggat ctccttctct ttttcaatct gccgcagtgc
     1681 atgtatgaac tcggctgtta ccatccagta cctccgactc gcgctctccc gactactcgc
     1741 accaaacccg atagcaagcg tgctcgatac cagtcctccc taaaaccaaa gagtgggagt
     1801 tctcccactc ttcctcggtg ccaacgcgat cacgcgataa ttagtctgga tgtattatag
     1861 catacggcga atccttttgc aagcgcgccc gaatccgcga acccggtcga ggggcgggcg
     1921 gccggtgtca gacggcgtcg ctttgcccgc gtgccggggg cgcctccgac tcctggcgct
     1981 tgcggcgact ggagagccac ccgcgcgcga agaggatcgc ctcaccgcgc gtgctcacct
     2041 ccccaaccgc gacggcctct ttgagggcat cgagcaaatc gcccaggagc gggccctgcg
     2101 ggcatccgac cgcctccgcc aactctcgcc cgccaatggg cacgacggga tgcgccacgg
     2161 tggctccctc caggctgagg cggagcatct cttctacaaa ccgacggtga cgctctacct
     2221 gggcatcggt ggcccacgga ccgcgcgccg ccagccggtc tgccagcgag aggagaagcg
     2281 attccacggt ggcgtcgccc atatcccgga agaagtggta gcgttgtcgc gccgccacgg
     2341 gctcgtggac ggagatgaag ccgggtcgaa ggtgctagcc aaccgccgtc accacccgct
     2401 tcgtctctcg cttgctcagg cgatagcgcc ggcagatggc gcccgccatt tcggctccca
     2461 ccacatcatg ccgggcgaag gtgatgatcc cctccggggt ctccccgcgt gtttgtggct
     2521 tggcgatatc gtggagcaga acagagaaga gaaggagcgc aagccgggat cgcggggcgc
     2581 agatcggctg ccggagctgt tccgcgaccg cggcgcgcag atcctccggc aactccggtc
     2641 cgatgagcag ggcttcacac tgtcgcatcg cctccagcgt gtgctcccag acatccaggt
     2701 ggtggttggc gttctgagtg aggcccttca gggcaaccgt ctcgggcagg atcggcgcga
     2761 gaaggccgag cgtctccatg cggcgcaccc acgtggcgct tcccggaacc tcgagaagcg
     2821 cgaaaagctc atcacgtatc cgttcgaccg cggattgcgg caggagcgcg gcatcacgcc
     2881 ggacgagcgc ctcggtttcg gggtcgagag tgaactccag ggcggcggcg atgcgcaccg
     2941 cgcgcaggag acgcaccggg tcggccgtga agatgtcggg atgggtatgg cggagccggc
     3001 gggcgtgcag atcggcgaga cccccgcagg gatccaccag gctttgctcc aggttcccgg
     3061 cgtcgaacgg caccgggagc gccatg
//
LOCUS       DAOSCV010000538         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20367_length_3085_cov_2.835974, whole genome shotgun sequence.
ACCESSION   DAOSCV010000538 DAOSCV010000000
VERSION     DAOSCV010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20367_length_3085_cov_2.835974"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLY56_12825"
     CDS             complement(<1..181)
                     /locus_tag="PLY56_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22407.1"
                     /translation="MSAPPKARLTWRSILLGLLLIPPNCYWIVQMERVRKGPYVTSIS
                     LFANALFVLVLLALVN"
     gene            complement(178..483)
                     /locus_tag="PLY56_12830"
     CDS             complement(178..483)
                     /locus_tag="PLY56_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22408.1"
                     /translation="MPQERDEIEEKIDEFLEGRPRSSYLAELRAALARRLEGTRAALK
                     QTEDPKEQEKLRKEIAEMERQDEVLAREELITEFVEDSVRATVSWSLLKPEDDEGEA"
     gene            complement(862..1020)
                     /locus_tag="PLY56_12835"
     CDS             complement(862..1020)
                     /locus_tag="PLY56_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22409.1"
                     /translation="MRWPVEIRVVLIGAAALVLYHAVGGSWPGAALAITFMLVYLAIL
                     GLERVVRR"
     gene            1227..>3085
                     /locus_tag="PLY56_12840"
     CDS             1227..>3085
                     /locus_tag="PLY56_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015378194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22410.1"
                     /translation="MIKHCAALLFFLSIVGADAQSWHHPLYLGNGELWRQRIPVTIYN
                     EMERDAAGEPVVLHVGKGDGEADLAGVPVSQIRLCNAAGQELLFAVTSPAGSLVTRGS
                     VPAGSTLTLPAECPARGTAHYYLYFDNPVAWAVPDFLNAAAGVRNGSVEEGTGDTPAH
                     WIHDRQDPEHQLFWTTENPRSGKRCLKTVVAEDAEPTWIATRQRDIHIIGGAKYVMRA
                     WVKAENVRGHAGWYIHVGNAQNPMMIAPMLSGGEGTYDWREVTAEFTAPPEANRASLG
                     TVLRGTGTAWFDDVSLECAEKPRLLARAGKPERLSLRRAGADAKWFDENPRDDITWSH
                     RVPIRVRNFSDEARDAHLAYVDLSGLMARLRGKIRPEAIRVTDGDRVVPHCLLGQGVL
                     IEGDIPARTERTYFIYLSADPRIRPGTTASYEALLDGPRNLARNASFEQGTALPDEWP
                     GGAEGESPAGTRMGLDEAGLFGKRCVRMQIPHDSKLAWTGWRQDVPVKPRRTYLYAAW
                     LKTRDIRNGSVQLHAHYRNMAGEHCESQKFAATGPALTGTNDWTSLSGLFTMPDDIAN
                     FQLHLTMLASGTVWHDGVLLAEVTPAETGVLEAREAPITTRLAIWAVNPLVKV"
BASE COUNT          519 a         1078 c          992 g          496 t
ORIGIN      
        1 cgttgacgag cgccaggagc accagcacga agagcgcgtt ggcgaagagg gagatgctgg
       61 tgacgtaggg gcccttgcgc acccgctcca tctggacgat ccagtagcag ttgggcggga
      121 tgagtagcag cccgagcagg atcgaacgcc aggtgagccg ggccttgggg ggcgcgctca
      181 tgcctccccc tcgtcatcct ctggcttgag gagcgaccag gaaaccgtgg cgcgcaccga
      241 atcctccacg aactcggtga tcagctcctc gcgcgccagc acctcgtcct gacgctccat
      301 ctcggcgatc tccttgcgca gcttctcctg ctctttcgga tcctccgttt gtttcagcgc
      361 agcgcgggtc ccctccaggc gccttgccag cgcggcgcga agctccgcca ggtagctcga
      421 gcgcgggcgc ccttccagga actcgtcgat cttttcctct atctcatcgc gttcctgcgg
      481 catctcaact cccaccctct ccccgatcgc ggggttgctt gccgccagat tcttccagta
      541 gagggcggtg tattcctgcc cgggcctacc tggcaccggt cagggaatcc caaacacggg
      601 cgggggagtt cggaggaagc agagcgttct ctgcattgag aggagccggc gtaagagctc
      661 aggatgatcg aggcgccctt acggagggtg ggtcatgcgt gccgcaccat gcggtgtcgg
      721 ggtttttctt taccccggcc cgctttcagc ttaccccgta tgggccaggt cttagatcgg
      781 gacaaagcgc acgcgggggg cctgccgcgc atcgggcctc tgtgcgcgaa tgctccccta
      841 catcccggcc cacacggggg cttacctccg gacaacccgc tccagaccca ggatcgccag
      901 gtagactagc atgaaagtga tggcgagggc cgctccaggc caactgccac ccaccgcgtg
      961 atagagcacc aatgccgccg cgcctatcag cacaacccgg atctctaccg gccatctcat
     1021 tgccattctc cctcgcgggc ggtcgtcttg tctcggctcg tcggccatgc acacctcctc
     1081 ggcagcgcgt gctgcctggc ccaacgggag tttagccact gacgccggtg ttcaaacgcg
     1141 ccggggctga ccagcgggtg ctaccatccc caccggcagg aatgctgctc cacgggtcta
     1201 accagactcg gcaagggggt ccaaagatga tcaagcactg cgcagcgctt ctcttcttcc
     1261 tctccatcgt tggggcggac gcccaaagct ggcaccaccc gctctacctg ggcaacggtg
     1321 agctatggcg ccagcgcatt ccggtcacca tctacaacga gatggaacgc gacgcggcgg
     1381 gcgagccggt cgtcctgcac gtgggcaagg gcgatggcga ggcggatctc gccggcgtgc
     1441 ccgtctctca gatccggctg tgcaacgccg cgggacagga gctcctcttc gccgtcacca
     1501 gccccgccgg ctccctggtg acccggggct cggtgcccgc cggatcgacg ctgacgcttc
     1561 ccgcggagtg cccggcgcgc gggacggcgc actactacct ctactttgat aatccggtgg
     1621 cctgggccgt gcccgatttt ctgaacgctg cggcgggcgt gcgcaacggg agcgtggagg
     1681 agggcaccgg cgacacgccg gcccactgga tccacgaccg gcaggatccc gagcaccagc
     1741 tgttctggac caccgagaac ccgcgctccg ggaaacgctg cctcaagacg gtggttgccg
     1801 aggatgccga gccgacctgg atcgccaccc ggcagcgcga tatccacatc atcggcggcg
     1861 cgaagtacgt gatgcgcgcg tgggtgaagg ccgagaacgt gcgcggccat gccggctggt
     1921 acatccacgt cggcaacgcg caaaacccga tgatgatcgc gccgatgcta agcggtggcg
     1981 aggggaccta cgactggagg gaagtcaccg ccgagtttac cgcgcccccg gaggcaaacc
     2041 gcgccagcct cgggacggtg ctgcgcggca ccggcaccgc ctggtttgac gacgtgagcc
     2101 tggagtgcgc ggagaagccg cgcctcctgg cgcgcgccgg caagccggaa cgcctctcct
     2161 tgcgtcgcgc cggcgcggac gcgaagtggt ttgatgagaa tccgcgggac gacatcacct
     2221 ggagccaccg cgtgccgatc cgcgtgcgca acttctcgga cgaggcgcgc gacgcccacc
     2281 ttgcctatgt ggatctctcc gggctcatgg cccggctgcg cggcaagatc cgccccgagg
     2341 cgattcgcgt caccgatggc gaccgcgtcg tgccccattg cctcctcggg cagggagtgc
     2401 tcatcgaagg agacattccc gcgcggaccg aacgcaccta cttcatctat ctctccgcgg
     2461 atccgcggat ccggccgggc accacagcca gctatgaggc gcttctggat ggcccgcgca
     2521 acctggcgcg caacgccagt ttcgagcagg ggaccgcgct ccccgacgaa tggcccggcg
     2581 gcgccgaggg cgagagtccc gccggcaccc ggatgggcct ggatgaggcc ggcctcttcg
     2641 ggaaacggtg cgtccgcatg cagatcccgc acgactcgaa gctggcctgg accggctggc
     2701 gccaagatgt gccggtgaaa ccgagacgca cctacctcta cgccgcctgg ctaaagacgc
     2761 gcgacatccg caacggctcg gtgcagcttc atgcccacta ccggaacatg gccggcgagc
     2821 actgcgagag ccagaaattc gccgccactg gcccggcgct caccgggacg aacgactgga
     2881 cctcgctttc cggcctcttc acgatgccgg acgatatcgc caacttccag ctccacctga
     2941 cgatgctggc gagcggcacg gtgtggcacg acggcgtcct tctcgccgaa gtcaccccgg
     3001 ccgaaacggg cgtgctcgag gcgcgcgagg ccccgataac cacccgcctg gccatctggg
     3061 ccgtgaaccc cctggtgaag gtgtt
//
LOCUS       DAOSCV010000539         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20374_length_3084_cov_4.260152, whole genome shotgun sequence.
ACCESSION   DAOSCV010000539 DAOSCV010000000
VERSION     DAOSCV010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20374_length_3084_cov_4.260152"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLY56_12845"
     CDS             <1..585
                     /locus_tag="PLY56_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22411.1"
                     /translation="RLKLISDVGPADDSSGDWAGWAEMRIETLAPKLYRILDPDAEAY
                     RRLPAPYPATGLTVADLRQAKSGWLRYDGKGLAGTGEYATEAVLNGISLGHMAPAGGS
                     ETNGIFEERVGVRLTPEAIRSLGVRNRFAIRNPRHDCFSVRRFWIELELADGRRASSE
                     ITAATYSQPVGWLYAEGISVPENAEITVDIWFRF"
     gene            800..1180
                     /locus_tag="PLY56_12850"
     CDS             800..1180
                     /locus_tag="PLY56_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ22412.1"
                     /translation="MSVPCRVLLVDDEEDACVVLGDVLECYGCEVRTVCDPAEALRIG
                     SSETFDLVVLDIRMPEIDGIEVLRRIRSRGHREIVMLTGFIDDDLEAKARHEGAAAVL
                     HKPVDVDALLARIDALRQETGRQA"
     gene            1352..1522
                     /locus_tag="PLY56_12855"
     CDS             1352..1522
                     /locus_tag="PLY56_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22413.1"
                     /translation="MPGELESAMYTGTSDGEPAPGPAELGPLPDREEVLEVFQEADRL
                     IAEQSEAEPQAG"
     gene            1522..1980
                     /locus_tag="PLY56_12860"
     CDS             1522..1980
                     /locus_tag="PLY56_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22414.1"
                     /translation="MAFRSNGGGMVTSYLETLREVAKHPVISQTVKRYSLEMGVVFPD
                     LTDPKGLMQLIESLFSPENSETVRSESGLERVLGLFIDEAKVRAAANEQASMALARTL
                     VLLEVYDGELARLGLDPGEKMDSLITKALLVELMLSAIRKYAFSVKAAHD"
     gene            complement(1994..2230)
                     /locus_tag="PLY56_12865"
     CDS             complement(1994..2230)
                     /locus_tag="PLY56_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22415.1"
                     /translation="MNQIPGDQSNPDDPRYPTKDEVDAAWLKLVVQPALLEEVRRALA
                     DRAQQGGRQEAPIRASPKERPSPLMWLLRLLSRR"
     gene            complement(2316..2960)
                     /gene="thiE"
                     /locus_tag="PLY56_12870"
     CDS             complement(2316..2960)
                     /gene="thiE"
                     /locus_tag="PLY56_12870"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOJ22416.1"
                     /translation="MSRSPDYSLYLVTDDALAGGRSIEWIVAEAVAGGVTCVQLREKH
                     AESRAYYERARALRERLRPLGIPLIINDRLDIALAAGADGVHLGQGDLPCVEARHIAG
                     PNFLIGISVSTTAEAQAAARDGADYLGISPIFDTPTKTDTPPATGIDGLRAIRAAVSL
                     PLVAIGGIKAENAAAVVRAGADGIAVVSAIMAAADPRAAARALADVVARARQQV"
BASE COUNT          520 a          969 c         1060 g          535 t
ORIGIN      
        1 cgcctgaagc tgatctcgga tgtgggcccg gcggatgatt cctccggcga ctgggccggc
       61 tgggccgaga tgcgcatcga gacgctggca ccgaagctct accgcatcct cgatccggac
      121 gcggaggcct accgccggct cccggcgccc tatcccgcga ccggcctgac cgtggccgac
      181 ctgcgccagg cgaagtcggg atggctccgc tacgacggca aggggcttgc cggaaccggg
      241 gagtacgcga cggaggcggt gctcaacggc atctccctcg ggcacatggc cccggcggga
      301 ggcagcgaga ccaacggtat cttcgaggag cgcgtgggtg tgcggctcac cccggaagcg
      361 atccgctctc tcggggtgcg caaccggttc gccatccgca acccgcggca cgattgcttc
      421 agcgtgcgcc gcttctggat cgagctagag ctggccgatg ggcggcgcgc ctcctctgag
      481 atcaccgcgg cgacctactc tcagccggtc gggtggctgt acgcggaggg catcagcgtg
      541 ccggagaacg ccgagatcac cgtcgatatc tggttccgct tctaacacgg cttcatgtcc
      601 gttcatcccc tgcgtgtggg cgagcccccg cggttcgccc acacgcggcc ctgttcaggt
      661 gcgcctggga gaccgacccg ggattttgct tctgaggctt tgcgcccacc gggatcaagg
      721 gggagcgcct ggtaatggaa cgtgccacga tgaggaggcc accgtaggga acactggtct
      781 ggcttgtggg gtagtctcga tgtctgtgcc atgccgtgtg ctgttggtag acgatgaaga
      841 ggatgcttgc gtcgttctgg gggatgtcct ggagtgctat ggatgcgagg tacgcaccgt
      901 gtgcgatccg gcggaggcgt tgcgtattgg atcctccgaa acgtttgacc tggtggtcct
      961 cgacattcgt atgccggaga ttgatggcat cgaagtgttg cgccggatcc gttcgcgtgg
     1021 tcaccgagag attgtgatgc ttaccggctt catcgatgat gatctggagg ccaaggcgcg
     1081 ccacgaaggc gccgccgcgg ttctccacaa gccggtggat gtggatgctc tcctcgcgcg
     1141 gatcgatgcg ttgcgccagg aaacgggtag gcaagcctga aggtaaccgc ctcgctgcgc
     1201 gcccgcccgc aaagggggat aaaacccggg ggctctggtg tgggagatgc ggttttcgcc
     1261 actcaaaacc ggggtaaatc agagtgacat ggcgaatacc agagggcgga acacccagga
     1321 accagaaacg ggaccagagc gaattgaacg attgccgggc gagttggagt cggcgatgta
     1381 caccggcacc agcgatggcg agccagcccc gggcccggcg gagctggggc cactcccgga
     1441 ccgggaggaa gtgctggagg tcttccagga agcggataga ctgatcgcgg agcaatctga
     1501 ggccgagccc caggctggtt gatggccttc aggtcgaacg ggggtggcat ggtgacatcc
     1561 tacctggaga cgctacggga agtggcaaag cacccggtga tctcgcagac ggtgaagcgc
     1621 tactcgctcg agatgggagt ggtcttccca gacctgaccg atccgaaggg gctgatgcag
     1681 ctgatagaga gcctcttttc gcccgaaaac tccgaaacgg tgcgttcgga gagcggcctg
     1741 gaacgggtgc tcggtctctt catcgacgag gcgaaggtgc gcgccgcggc gaacgagcaa
     1801 gcctcgatgg cccttgctcg taccctggtc ctcctggaag tgtacgatgg cgagctggcc
     1861 cgcctgggcc tcgaccccgg cgagaagatg gatagcctga tcaccaaggc gcttctggtc
     1921 gagctcatgc tcagcgcgat ccgaaaatac gccttctccg tcaaggccgc gcacgattga
     1981 ttcagcgggc gctttaccgc ctgctcaaca agcggagcag ccacatcaac ggcgatggcc
     2041 gctccttcgg actggcgcgg atcggtgcct cctggcgccc gccctgctgg gcgcggtccg
     2101 caagggctct cctaacctcc tccagtaacg ctggctgtac gaccagtttc agccaggcgg
     2161 catcgacctc gtccttcgtt gggtagcgcg ggtcatcagg attcgattgg tctccaggta
     2221 tctggttcac gggttcccct tcccccaacg cggggcgtcg cgaggagttg ctgccccttg
     2281 cagcagcgac gccccttacc agatacgtgg caccattaca cctgctggcg tgcgcgagcc
     2341 acaacatccg ccagcgcccg cgcggcggca cggggatccg ccgccgccat aatggcagag
     2401 acgacggcga tgccatctgc gcccgcgcgg accacggccg ctgcgttttc cgccttgatc
     2461 cccccgatgg caacgagggg aagggagacg gcggcgcgga tggcacgcag gccgtcgatc
     2521 cccgtggccg gaggcgtgtc cgtcttagtt ggcgtgtcga agatcggact gattccgagg
     2581 tagtcggcgc cgtctcgcgc ggcggcctgt gcctccgccg tggtgctcac ggagatgccg
     2641 atgaggaagt tggggccggc gatgtgccgt gcctcgacgc aggggagatc cccctggccc
     2701 aggtgaactc catcggcgcc ggcggccagt gcgatatcga ggcgatcgtt gatgatgagc
     2761 gggattccca ggggacggag ccgctcgcgc agagccctgg cgcgctcgta gtaagcgcgg
     2821 ctctccgcat gcttctcgcg cagctggaca cacgtgaccc cgcctgccac cgcctcggcc
     2881 acgatccact cgatgctccg cccgccggcc agtgcatcgt ccgtgacgag atagagcgag
     2941 tagtcgggcg agcggctcac tccgcggcct cctccacctt gattcgcgcg ccctcgcgca
     3001 cgtgcggggc actcacttca tccagggcgt caaacaggcg gattccgaag gtgccagggc
     3061 ggggggcgcc cgatgcggcg atct
//
LOCUS       DAOSCV010000540         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20437_length_3076_cov_3.199272, whole genome shotgun sequence.
ACCESSION   DAOSCV010000540 DAOSCV010000000
VERSION     DAOSCV010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20437_length_3076_cov_3.199272"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="PLY56_12875"
     CDS             <1..1605
                     /locus_tag="PLY56_12875"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOJ22417.1"
                     /translation="NRDLRRYEEHVGGLRERFRAEEMRFLESSPAVNGEILYTGGDED
                     ADEAINRYYQHVTATERRQIDDRVQAELGITSMGLYGYFLAEGAGSIDTIAGTILRHT
                     TAAFKDDIEAVEVLNLFFERYPDDRAAEEQMKRLNRLCSPFVHAKAGGMAGFTDDPQM
                     RQRNIGIPNAEEPKTSAERRFRAMVTKAASVTDPRTWVTVASRSEALFLHERSGFPLR
                     LMEDALRRCESHYEHTRKRRKEPVHARVDFQQWESLTPAPPHLKAEAWKIFFIAAASG
                     VLTCRPVEVQTQRGQEERFSYEISYFDDQGVQHTEVVADRLPPFDALRFDGRDYPNGV
                     MQLLLKLCHPEGLTDLARRIQRKQEETVAKEGVAAIRERLLDLVNNRLSPQAEFTPHA
                     CEVVSGYLRSLQSAPGAGAPATPPAPAPAGATGDRLAKLRGLLEKRLMTASDYAAKLK
                     EMRGEGVLTSDECRASLNELRDAGLLTPAEYDAACQALGLETAESPAEENSIEARLMK
                     LKSLFEKGLITQEDYDAKRTAILAEI"
     gene            1648..2454
                     /locus_tag="PLY56_12880"
     CDS             1648..2454
                     /locus_tag="PLY56_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22418.1"
                     /translation="MSESDRLRRLRKLREALDAPLVPRGTSRASAAPYPTVPPRYTYP
                     GPVVPGTRAELAAAPKGSRPLRWLAAALSPLAALERWHLQAATVALAILGALNAYWFQ
                     WGLEKAAITIIATAALPALLSGLCDLLFRLTVIPGIRLHVALLQWARERHRALVIVRA
                     WADTPLRGRWKRAVQVQFASGAIPARTLDVVTDLYDDNSKLDIALLVEAPALPLDDLL
                     VWVRVPDGRLAGMEASIRRFREERCARCLGEETDCALCGGTYRVPEAQFL"
     gene            2796..>3076
                     /locus_tag="PLY56_12885"
     CDS             2796..>3076
                     /locus_tag="PLY56_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22419.1"
                     /translation="MEQRRRGLFSPRAVACAAALLLLGSPAGAAPEKPAPKPASLSPT
                     NPGFEKELVGWTLERPLSPEQGRVSVVEGDAAEGKHAAVLRAHSSRVTVG"
BASE COUNT          508 a          997 c         1100 g          471 t
ORIGIN      
        1 aaccgcgatc tgaggcgcta cgaggagcat gtaggcgggc tgcgcgagcg cttccgtgcc
       61 gaggagatgc gcttcctgga atcctccccg gccgtcaacg gtgagatcct ctacaccggc
      121 ggagatgagg acgcggatga agcgatcaac cgctattacc agcatgtgac cgcgacagag
      181 agacgccaga tagacgaccg cgtccaggcg gagctgggca tcacctccat gggcctctac
      241 ggctacttcc tggccgaggg cgccggctct atcgacacga ttgcaggaac tatcctgcgg
      301 cacacgacgg cggcgttcaa agacgacatt gaggcggtcg aagtgctcaa tctcttcttc
      361 gaaaggtacc cggacgaccg cgccgccgag gagcagatga agcgcctcaa ccgcctctgc
      421 tcgccattcg tccacgcgaa agcgggcggg atggccggct tcaccgacga cccgcagatg
      481 cgccagcgca acatcggcat ccccaacgcc gaggagccga agacatcggc ggagcggcgc
      541 ttccgggcga tggtcaccaa agccgcgtcg gtgaccgacc cgcgcacctg ggtgacggtg
      601 gcctcgcgca gtgaggcgct cttcctccat gagcgttccg gctttcccct gcggctcatg
      661 gaggacgcgt tgcgtcggtg cgaaagccac tacgaacaca cgcgcaagcg ccggaaagag
      721 ccagtgcacg cccgcgtgga tttccaacag tgggagagtc tcacgccggc gccgccacac
      781 ctgaaggcgg aagcgtggaa gatcttcttc atcgcggcgg cttccggcgt gctcacgtgc
      841 cggccggtgg aggtgcagac ccagcgcggc caggaggagc gcttcagcta cgagatcagc
      901 tatttcgatg accagggcgt gcagcatacc gaggtggtgg ccgaccgcct cccgcccttc
      961 gatgccctcc gcttcgatgg gagagactat ccgaatggcg tgatgcagct cctcctgaag
     1021 ctgtgtcacc ccgagggatt gaccgatctg gcccggcgca tccagcgcaa gcaggaggag
     1081 actgtggcca aagaaggggt ggcggcgatc cgggagcgcc tcctcgatct cgtcaacaac
     1141 cggctgtcgc ctcaagccga gttcacgccg cacgcgtgcg aggtggtgag tggctacctc
     1201 cgctcgcttc agagcgcgcc aggagcgggc gcgcccgcga ctcccccggc gccggcaccc
     1261 gcgggcgcta caggcgaccg gctcgcgaaa ctgcgcggcc tcctcgagaa gcgcctgatg
     1321 accgcttcgg actacgccgc gaagctgaag gagatgcgcg gcgagggcgt gctcacatcg
     1381 gatgagtgcc gggcgagcct gaatgaactc cgcgacgccg gtctcctgac gccggcggag
     1441 tacgacgcag cttgccaggc gctcggcctc gaaacggcgg agagcccggc ggaagagaac
     1501 tcgattgagg cccgcctgat gaagctgaag agcctcttcg agaagggatt gatcacccag
     1561 gaggattacg acgcgaagcg aaccgcgatt ctggcggaaa tttgagaccg tgccacgttt
     1621 ggttgctggt gagtggaggc cggtgcagtg agtgagtcgg accggctgag gcggcttcgc
     1681 aagctgcggg aggcgctcga tgcgccgctc gtgccgcgcg gcacgtcgcg cgcttccgcc
     1741 gccccctatc cgaccgtgcc gcctcggtac acctacccgg gtccggtggt accgggcacg
     1801 cgcgcggagc tcgccgcggc gccgaagggc tcgcgcccgc tccggtggct cgcggccgcg
     1861 ctctcgccac tcgccgctct ggagcggtgg cacctgcagg cagccacggt cgcgctggcg
     1921 atcctaggcg cgctgaacgc ctactggttt cagtggggcc tggaaaaggc ggcgatcacc
     1981 atcatcgcca cggcggcgct gccggcgcta ctcagcggcc tctgcgacct ccttttccgg
     2041 cttaccgtca ttccggggat tcgcctgcac gtggcgctgc ttcagtgggc gcgcgagcgc
     2101 catcgcgcgc tcgtgattgt gagagcctgg gccgacaccc ctctccgggg gcgctggaaa
     2161 cgggcggtgc aggttcagtt cgcctcgggg gcgatcccgg cgcggacgct cgacgtggtg
     2221 acggacctct acgacgacaa ctcgaagctg gatatcgccc tcctggtgga ggcgccggca
     2281 ctccctttgg acgacctcct tgtctgggtg cgcgtgccgg atggccggct ggctgggatg
     2341 gaggcgagca tccgccggtt tcgagaggag cggtgcgcgc gctgcctggg cgaggagaca
     2401 gactgcgccc tctgcggcgg cacctaccgg gtgccagagg cgcaattcct gtgatttgcc
     2461 ctttcgcggg cagagggcac gaccggccgc tttcggaaac gccgcgtgcc cggcggtgga
     2521 aacccggggc cgcgtgtatc gcgcggcgat agatcaccgc gctgcctgca agcgaagccc
     2581 tgccgggctg gtgatggctg tctgcagtgc aaagtggatg ggcagggttg ggaggggaag
     2641 ccccgaaggg gctttgcagg gaagcagcgc ggagcttcca gctccgcgtg cgcgtgttgg
     2701 ccacgcacct gcgcaacggc ggttggctgg gcctgggatg tcgcgataga cgcgcgcggg
     2761 gtaggctccc aggggacagg gaggagatgg cggagatgga acaacgccgg cgagggctgt
     2821 tctcaccgcg ggcggtcgcg tgcgcggcag ccctgttgtt gctcgggagt ccggcgggcg
     2881 ccgcgcctga gaagcctgcg cccaaaccag cttccctctc gccgaccaac cccgggttcg
     2941 agaaagagct ggtgggatgg acgctcgagc gtcccctcag ccctgagcag ggccgcgtga
     3001 gcgtggtcga gggcgacgcc gccgagggga agcacgcggc ggtgctgcgc gcgcactcat
     3061 cgcgggttac ggtggg
//
LOCUS       DAOSCV010000541         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20478_length_3071_cov_4.597812, whole genome shotgun sequence.
ACCESSION   DAOSCV010000541 DAOSCV010000000
VERSION     DAOSCV010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20478_length_3071_cov_4.597812"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="PLY56_12890"
     CDS             <1..738
                     /locus_tag="PLY56_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22420.1"
                     /translation="MHEDQWAVVEELSEQAARTPWRLQGIYERTKGDFAAALREAERV
                     MRRYVRLKADQEATRAAWDAAIERRKREIERLERGKERACAKYDWDLSYCLALLDGFM
                     ESFMHHLPRKASFETPAGKFQRRACRPTTEKAPEHAVLPLLQQLPPEAQQGAITLKPQ
                     VNWAWIKEHLVETEEGLVLRWIHPDTGEVVSIPAVVERETAYGRIEATPILRTVEPAS
                     PYTMKVVPAEGIRDDELETESTEATEE"
     gene            735..1271
                     /locus_tag="PLY56_12895"
     CDS             735..1271
                     /locus_tag="PLY56_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22421.1"
                     /translation="MTTGLTFRRATKARARARIALIGPSGSGKTYTALLFAQTLGERV
                     ALIDTEHGSASKYADVFTFDVLELTDFAPKAYVEAIEAAGAAGYDVLIIDSLSHAWAG
                     KGGALEMVDNAAARPGCNKYTAWRDVTPWHNRLIDTIIASPCHVIATMRAKTEYVIVE
                     AEAKALRDRLAEIEARLD"
     gene            1293..2252
                     /locus_tag="PLY56_12900"
     CDS             1293..2252
                     /locus_tag="PLY56_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22422.1"
                     /translation="MTTGLTFRRATKARARARIALIGPSGSGKTYTALLFAQTLGERV
                     ALIDTEHGSASKYADVFTFDVLELTDFAPKAYVEAIEAAGAAGYDVLIIDSLSHAWAG
                     KGGALEMVDNAAARPGCNKYTAWRDVTPWHNRLIDTIIASPCHVIATMRAKTEYVIEK
                     DEKGNTTIRKVGLAPVQRDGMEYEFDIVADMDASNTLMVSKTRCPALRQATVRMPGAE
                     TAEVIKAWLSDGVDAPEPPPSYAPATKAAAPGPADRVRLLQRNLNTDLSLPADDIIAA
                     TKAIRKQIAPEAEKLEDLTEEQAEAICLALEEWAFNVHGATTV"
     gene            2267..2689
                     /gene="ssb"
                     /locus_tag="PLY56_12905"
     CDS             2267..2689
                     /gene="ssb"
                     /locus_tag="PLY56_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ22423.1"
                     /translation="MPKTVLWGNLGRDGELCAGQNAQFLKLAVAEHVQKAGSGNGEEA
                     DDRQTIWWNVLVWQPMASALAPFMERGLRVCVMGRVDVSTWTDGEGAVRLNRTIIADA
                     VGWQSKRKRDEAEAAETEVLPLQQQGSNHEDDDPFGDQ"
     gene            2776..>3071
                     /locus_tag="PLY56_12910"
     CDS             2776..>3071
                     /locus_tag="PLY56_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22424.1"
                     /translation="MNTAAALAMLVSFVASPVQPLPKDPFQRTAVRALRGDFGTLQPW
                     QREGYERGLRAGATAHKTIWLTGYYATEGRSGRTDCQGRRCSLRTAAANRVAQGA"
BASE COUNT          623 a          931 c         1077 g          440 t
ORIGIN      
        1 atgcatgagg accaatgggc ggtagttgag gagctgagcg aacaggcagc gcgcacgccg
       61 tggcgcctcc agggcatcta cgagcgcacg aaaggcgact tcgcagcggc gctgcgcgag
      121 gcggagcggg tgatgcgccg ctatgtgcgg ctcaaggcgg accaggaggc cacacgcgct
      181 gcctgggacg ccgccatcga acgccgcaaa cgagagatcg agcgcctgga gcgcggcaag
      241 gagagggcct gcgcgaagta cgactgggac ctgtcctact gtctcgcgct gctcgacggc
      301 ttcatggagt cgttcatgca tcacctgccg cggaaggcgt cgtttgagac gccggctggg
      361 aagttccagc gccgagcctg tcgacccacg acggagaagg cgccggagca cgcggtgttg
      421 ccactgctgc aacagctccc gccggaggcg cagcaaggcg ccatcactct caagccgcag
      481 gtgaactggg cgtggatcaa ggagcatctg gtggagaccg aggagggcct cgtcctccgc
      541 tggatccatc ccgacaccgg cgaagtggtg agcatcccgg ccgtcgtgga gcgagagact
      601 gcctatggtc gcatcgaggc gacccccatc ctgcggaccg tagagcccgc gagtccatac
      661 accatgaaag tcgtccctgc ggaggggatc agggacgacg agcttgagac tgaaagcacg
      721 gaggcgacgg aggaatgaca acgggtctga cgttccggag ggcaaccaag gcccgcgccc
      781 gggcccgcat cgcgctgatc gggccctcgg gcagcgggaa gacatacacg gcgctgctgt
      841 tcgcgcaaac gctgggcgaa cgagtggcgc taatcgacac cgagcatggc agtgccagca
      901 agtacgccga cgtgttcacc ttcgacgtgc tggagctcac tgactttgcc ccgaaggcat
      961 acgtcgaggc cattgaggcc gcgggagcag ctggctacga cgtgctgatc atcgactccc
     1021 tatcgcacgc ctgggcgggt aagggcggtg ctttggaaat ggtggacaat gccgcggcgc
     1081 gtcctggctg caacaagtat accgcatggc gggacgtgac gccttggcac aaccggctga
     1141 tcgacaccat catcgcaagc ccctgtcacg tcatcgcgac catgcgcgcc aagaccgagt
     1201 acgtgatcgt ggaggccgag gccaaggcgc tgcgggaccg cctggccgag attgaggcgc
     1261 ggctggactg aaagcacgga ggcgacggag gaatgacaac gggtctgacg ttccggaggg
     1321 caaccaaggc ccgcgcccgg gcccgcatcg cgctgatcgg gccctcgggc agcgggaaga
     1381 catacacggc gctgctgttc gcgcaaacgc tgggcgaacg agtggcgcta atcgacaccg
     1441 agcatggcag tgccagcaag tacgccgacg tgttcacctt cgacgtgctg gagctcactg
     1501 actttgcccc gaaggcatac gtcgaggcca ttgaggccgc gggagcagct ggctacgacg
     1561 tgctgatcat cgactcccta tcgcacgcct gggcgggtaa gggcggtgct ttggaaatgg
     1621 tggacaatgc cgcggcgcgt cctggctgca acaagtatac cgcatggcgg gacgtgacgc
     1681 cttggcacaa ccggctgatc gacaccatca tcgcaagccc ctgtcacgtc atcgcgacca
     1741 tgcgcgccaa gaccgagtac gtgatcgaga aggacgagaa gggcaacacc acgatccgca
     1801 aggtcggcct ggcgccagtg cagcgggacg gcatggagta cgaattcgac attgtcgccg
     1861 acatggatgc cagcaacaca ctgatggtga gcaagacgcg gtgccccgct ctgcgccagg
     1921 cgacggtccg catgccaggt gcggagacgg cggaggtcat caaggcttgg ctcagcgatg
     1981 gtgtggatgc accggagcca cctcccagct atgcaccggc cacgaaggct gcggctccag
     2041 gtcccgccga ccgtgtgcgt ctgctgcagc gcaatctcaa cacggacctg agcctgccag
     2101 cggatgacat catcgccgcc accaaggcaa tccgcaagca gatcgccccg gaggccgaga
     2161 agctcgagga cctgacggag gagcaagcgg aggcgatctg cctcgcgttg gaggagtggg
     2221 cattcaatgt gcacggtgcg acgaccgtgt agggagctga tgagcgatgc cgaagacggt
     2281 gctatggggc aatctcgggc gggatggcga gctctgtgca ggccagaacg cacagttcct
     2341 aaagctcgcg gtggccgaac acgtgcagaa ggccggctcg ggcaatggcg aggaagccga
     2401 tgaccgacag acaatctggt ggaacgtgct ggtatggcag ccgatggcct cggcactcgc
     2461 gccattcatg gaacgcggtc tgcgtgtctg cgtgatggga cgggtggacg tcagcacgtg
     2521 gacggacgga gagggcgcag tacgcctgaa ccgcacgatc attgcggacg cggtcggttg
     2581 gcagagcaag cgcaagcgag acgaggcgga ggccgctgag acagaggtcc tgccactgca
     2641 gcaacagggc agcaatcacg aggacgacga cccgttcgga gatcagtagc gagggactgg
     2701 ggccgcttgg aaaccggcgg ccccgcgcat gctccagaag gacacatcga tatgaactgc
     2761 atgggaggcg gagcaatgaa cacggctgca gccttggcca tgctcgtgag tttcgtggcg
     2821 tcgcccgtcc aaccgttgcc caaggatccg ttccagcgta cggcggttcg cgccctgcgc
     2881 ggcgacttcg gaacgctcca accctggcag cgcgagggct atgaacgcgg cctacgggct
     2941 ggcgcgacgg ctcacaagac aatctggctg actgggtact acgccacgga gggccgatct
     3001 gggcgcacgg actgccaggg gcggcgctgc agcttgcgga cggctgccgc gaaccgcgtc
     3061 gcccagggcg c
//
LOCUS       DAOSCV010000542         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20520_length_3067_cov_3.581673, whole genome shotgun sequence.
ACCESSION   DAOSCV010000542 DAOSCV010000000
VERSION     DAOSCV010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20520_length_3067_cov_3.581673"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1055
                     /locus_tag="PLY56_12915"
     CDS             276..1055
                     /locus_tag="PLY56_12915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HOJ22425.1"
                     /translation="MVGTPPIDPNSQEAIVRRQDAGRLTWCAPALMLFARSALAVAAQ
                     AVVAVIFALRGAPTPWRDAEPWLPVYGTLIDAGCLALLWWFTRREGIGLFELVGFQRA
                     RILRDVLLGLVLIPVSLVFIFGGTYAAAWIVYGTLKLPLLFGDLPFPAALYGVLVWPF
                     IWGLTEQMTYNGYLLPRFQVLARSTSLAVAVVAFVWSLQHSFMPLTLDTEFMTFRLLA
                     AVPNTLFQTLVYLRLRRLVPLVVAHALMDGATVLFPLLARS"
     gene            complement(1176..2393)
                     /locus_tag="PLY56_12920"
     CDS             complement(1176..2393)
                     /locus_tag="PLY56_12920"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019177240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ22426.1"
                     /translation="MQSFHTADEAEIRAGATTDIYFRHTEEVLRQRGLLERRAHAEVT
                     TGGLPENWPWGVLCGLDEALSLLEGREVDIDALPEGTLFPARDRRGRRIPVLTLEGPY
                     GAYCIYETPLLGFLCQATGVATRAARIRLAAGDASVLSFGARRMHPAIAPMIDRAAWV
                     GGCDGISCILSAERLGITPTGTMPHALMILFEDQAEAWRAFDETMPPEVPRIALVDTY
                     WDEKAEAIIAAETLGEALYGVRLDTPGSRRGNFVDIVREVRWELDLRGFNHVRIFVSG
                     SLDEKVIPALAAAGAVGFGVGTHLSNAPTVDFALDIVSLEGAPVAKRGKLSGRKQVYH
                     CSGCGEWEVDLREMPPPACPGCDTPMRPMLRRWLDGGRRTAPTPSPTEIREYVLRQLS
                     GATLDGSPVSRGR"
     gene            complement(2526..>3067)
                     /locus_tag="PLY56_12925"
     CDS             complement(2526..>3067)
                     /locus_tag="PLY56_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOJ22427.1"
                     /translation="APDHHVNAAHTPAAPAARRAATGSCPGAAMRQFAPRPSAGQPTP
                     GTPSALRQWPVQLHLVWPEAPYFRDADLLLAADCSAFACGDFHARFLDGRALAIACPK
                     LDDPDGYVEKLAAMIAHAGLRSITIVMMEVPCCHGLDRLVQLAMQKANRQVPVRRVIV
                     SIEGEIVYDEGEAVRVETE"
BASE COUNT          481 a         1000 c         1027 g          559 t
ORIGIN      
        1 ccggggtcct tcaagggctg tcgcatctac tcgcggcagc ccttttgctt gtacctcgtc
       61 cttcgcctac aactgtgaga tcagagacaa gccgtcgccg gtggacaggg tcctggaact
      121 gagcgagttc tcgctcatcc agtttgacgg cccgaactca tactgggtga catcccaggt
      181 cggcggcatg cgggagtact tcaagcgggt ccgatagccg ccggcgcaaa ggcgcgcgcg
      241 ttttgccggt gccggcacgg ggaactccac tagggatggt tggaacaccg cccatagatc
      301 cgaattccca ggaggccatc gttcggcggc aggatgcggg aaggctgacg tggtgcgcgc
      361 ccgcgctgat gctgtttgcg cgctctgcgc tcgccgtggc cgcgcaagcc gttgtcgccg
      421 tcatcttcgc cctgcgcggg gcgccgactc catggcgtga cgctgagcca tggctgccgg
      481 tgtacggcac tctcattgac gccggatgcc tggcgctgct ctggtggttc acgcgccgcg
      541 agggaatcgg cctgttcgag ctggtcggtt tccagcgggc acgcatcctc cgggatgtgc
      601 tgttgggcct tgtgctcatc cctgtgagcc tcgtgttcat tttcggtggc acctatgcgg
      661 cggcctggat cgtctatggc acgctcaagc tgccgctcct ctttggagac ctgccatttc
      721 cggctgctct ctatggcgtg ctggtgtggc ccttcatctg ggggctgacc gagcagatga
      781 cctataacgg ttatctgctg ccgcgctttc aagttctcgc ccggagtacg agcttggcgg
      841 tcgcggtcgt cgcgttcgtg tggtccttgc agcattcttt catgccgctg acactggata
      901 cggagttcat gacgtttcgg ttgttggcgg cggtgccaaa cacgctcttc caaacgctgg
      961 tgtacctgcg acttcgccgg ctggttccgc tcgtggtggc gcacgccctg atggatggag
     1021 cgaccgtgct cttccccttg ttagcgagat cataacgctt ggggaacagg tcgctgcggg
     1081 tcattaggac aagggttggc gttcgcacgc tcgcgcaagc cagtcggatg agcgccatga
     1141 cgggccagcc tgatggatcg gggactccat gacgctcaac gtcctctgga gacgggtgat
     1201 ccgtcaagag ttgctcccga gagctgccgg aggacgtact cccggatctc cgtcgggctc
     1261 ggggttggcg cggtgcgccg gccgccatcc agccaccgcc gcagcatggg gcgcatcggc
     1321 gtgtcgcatc cggggcaagc cggcggtggc atctcccgga ggtcaacctc ccattcgccg
     1381 caaccggaac agtgatacac ttgcttgcgc cccgagagct tcccgcgctt cgcgacgggc
     1441 gcaccctcca acgagacaat gtctagcgcg aaatcaaccg taggcgcgtt gctcaggtga
     1501 gtgcccacgc caaagccgac cgcgccggcc gcggcaaggg ctgggataac cttctcgtcc
     1561 agcgatccgg agacgaagat ccgcacgtgg ttgaagccgc gcagatccag ctcccagcgc
     1621 acctcgcgta caatatcgac gaagttgcct cggcgcgagc caggggtatc caggcgcacc
     1681 ccgtagaggg cttcccccag cgtttcagcg gcgatgatcg cctcggcttt ctcgtcccag
     1741 taagtatcga ccagagcgat gcgtggcacc tcgggcggca tcgtctcatc gaacgcgcgc
     1801 cacgcctccg cctgatcctc gaagaggatc atcagcgcat gaggcatggt gccggtgggc
     1861 gtgattccca ggcgctccgc actcaagatg caggagatgc cgtcgcaacc gcccacccag
     1921 gcggcacggt cgatcatggg agcgatggcg gggtgcattc gccgggcgcc aaaggagagg
     1981 acggaggcgt cgcccgctgc caggcggatc cgggcggcgc gcgtcgcgac cccggtggcc
     2041 tggcaaagga agccaagaag gggcgtttcg taaatgcaat aggccccgta cggcccctcg
     2101 agggtgagca ccgggatacg ccggccacgc cggtcgcgcg ccgggaaaag cgttccctcc
     2161 ggcagcgcgt cgatgtctac ctcacgcccc tccagcaggc tcagcgcctc gtcaaggccg
     2221 cacagaaccc cccacggcca gttctccggt aggccgcccg tggtcacctc ggcatgggcg
     2281 cgccgctcca acaacccgcg ttggcgcagg acctcctccg tgtgccggaa gtagatatct
     2341 gtcgttgccc ccgccctgat ctcggcttca tccgcggtat gaaagctctg cattggcctc
     2401 tcccgcggag ccatacccct cgcggggggt acggaaacga ggccgcgcgg gagggcgcaa
     2461 agtgcggtgg cgatgcgtcc aggcgttggc cgcactctgc gcccgatgcc gtcaagcggt
     2521 cacggtcact ccgtttccac ccgcacggcc tctccctcat catacacaat ctcgccttcg
     2581 atgcttacga tgacgcgtcg gacgggcact tggcggttgg ctttctgcat cgccagttgg
     2641 acaaggcgat ccaggccgtg gcagcaaggc acctccatca tgacgatggt gatgctgcgc
     2701 aggccggcgt gggcgatcat cgctgccagc ttctcgacgt agccgtcggg atcgtctagc
     2761 ttggggcagg cgattgccag agcccggcca tccagaaagc gcgcgtggaa gtcgccgcac
     2821 gcgaatgcgg agcagtccgc ggcgaggagc aggtcggcgt cccggaagta cggcgcctcc
     2881 ggccagacga ggtggagttg caccggccac tgacgcaggg cgctgggtgt gcctggcgtt
     2941 ggctggcctg ccgacgggcg tggcgcaaac tggcgcatcg ccgcgccggg gcacgagccg
     3001 gtggctgcac ggcgggccgc tggcgcagcc ggggtgtgcg ccgcgttcac gtgatggtca
     3061 ggcgcgg
//
LOCUS       DAOSCV010000543         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20524_length_3067_cov_2.754980, whole genome shotgun sequence.
ACCESSION   DAOSCV010000543 DAOSCV010000000
VERSION     DAOSCV010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20524_length_3067_cov_2.754980"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="flgA"
                     /locus_tag="PLY56_12930"
     CDS             <1..327
                     /gene="flgA"
                     /locus_tag="PLY56_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOJ22428.1"
                     /translation="RVEQRDVAGIAQPLAPNAEIVGLRVRRMLSAGSVLTASCVEATP
                     DVHRGDTITVSLRAGAIRVRVPAKALEEGAAGGEIRVVNLTGGREYRVRVTGPGTAEA
                     ILGPGE"
     gene            398..988
                     /locus_tag="PLY56_12935"
     CDS             398..988
                     /locus_tag="PLY56_12935"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOJ22429.1"
                     /translation="MRKTRATSICLVLGLSALTSLASADSIWNSAGTRRAPLIADQRA
                     HQAGDLLTVLIVESAAASQQANTQAEKKHSHQVSPSTGVLKFLPEAGFNDAHKFTGQG
                     ATSRSSNLTARMTVQVVRVQPDGMLEIEGTRTVVINEEKQELRLTGRVRPEDVSPENT
                     VLSSLIADAQITYTGKGPIGKAQKPGILTRLLRVFF"
     gene            1000..2139
                     /locus_tag="PLY56_12940"
     CDS             1000..2139
                     /locus_tag="PLY56_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOJ22430.1"
                     /translation="MRIILLMLALCTIASGLAAQPQSPQAAAPTAPRPAIQTRIKELA
                     RIAGVRNNQLFGYGLVVGLDSSGDSNQVAFTARAVANMMQRFGITLSSEQLKTRNVAA
                     VIVTADLPAFTREGDRIDVLVSSVGDARSLQGGTLLQTPLQGADGEVYAVAQGPVSIG
                     GFAAGGPGANISRNHPTAGRIPNGAIVEREVPVTAVEDGFLSLRLMRPDFSTAARMVD
                     AINRRLGSGTATPVDAGAIRVRIPSGQERNLVALVAQLEAIPVEPDMPARVIVNERTG
                     TVVIGGHAVIAPVAVSHGSLVVEIAQEPVISQPSPLGEGETVVTQKEQTRVQEEEATV
                     FPLQAATVEDLVKALNALRLTPRDIIAILQAIKQAGALQAELEVI"
     gene            2139..2483
                     /locus_tag="PLY56_12945"
     CDS             2139..2483
                     /locus_tag="PLY56_12945"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOJ22431.1"
                     /translation="MLQRIGALVAGQAGATGNGQQKALDKACEEMEALFLKQLLSEMR
                     RASGTTLRGPGGEIYQELFDQELARHLAGASDLGLARMVQQQLQRSAAPASPQQIPGG
                     AGAVEPNRSRPI"
     gene            2562..2870
                     /gene="flgM"
                     /locus_tag="PLY56_12950"
     CDS             2562..2870
                     /gene="flgM"
                     /locus_tag="PLY56_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOJ22432.1"
                     /translation="MRIQGREAVLQAYVSRVSEKRAGQVDRSTPTIEQEDRVTLSRSA
                     QDVRRVKEHLANLPEVRENRVRELRQLVERGEYHVSEEELADRILESGVLDLPVDFLI
                     "
     gene            2884..>3067
                     /gene="fliW"
                     /locus_tag="PLY56_12955"
     CDS             2884..>3067
                     /gene="fliW"
                     /locus_tag="PLY56_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOJ22433.1"
                     /translation="MRIQTTRFGPLDVEASSILRIHPGLLGFETRHRYVLLQTEENPL
                     FQWLQCVDDPALAFVVV"
BASE COUNT          530 a          988 c         1040 g          509 t
ORIGIN      
        1 cgcgtcgagc agcgcgatgt cgccggaatc gcccagccgc ttgcgcccaa tgcggagatt
       61 gttggcctgc gcgtccgccg gatgctttcg gcgggtagcg tgctcaccgc gtcctgcgtg
      121 gaggccacgc cggatgtgca tcgcggcgac acgatcacgg tctctcttcg cgccggcgcc
      181 attcgggtgc gcgttcctgc gaaagccctg gaggaaggtg ccgccggtgg ggagatccgg
      241 gtcgtcaacc tcacgggagg ccgcgagtac cgcgtccgcg tgaccggacc cggcaccgcc
      301 gaggcgattc ttggcccggg agagtagcct gccatcaggg ccgcgttttc caccgccgcg
      361 tcgttccggg gcagccatcc ccggggagag taaatggatg cgtaagacaa gagcaacctc
      421 tatctgcctg gtgctagggc tctccgccct gaccagcctg gcgagcgcag actccatctg
      481 gaactcggca ggaacacgcc gcgctccctt gatcgcggat cagcgcgcgc atcaggccgg
      541 ggacctcctg accgtcctga tcgtcgaatc ggcggcagcc tcgcagcagg ccaacacgca
      601 ggccgagaag aagcactctc accaggtctc cccctccact ggggtgctca agttcctgcc
      661 tgaagccggc ttcaacgatg cccataagtt caccgggcag ggcgcgacga gccggtcatc
      721 caacctcacc gctcggatga ccgtccaggt ggtccgcgtg cagccggatg gcatgctgga
      781 gatagagggc acgcgcacgg tggtgatcaa tgaggagaag caggagctgc gcctgaccgg
      841 ccgcgttcgt ccggaagatg tctccccgga gaacacggtc ctgtcaagcc tgatcgccga
      901 cgctcagatc acctacacgg gcaagggtcc catcggcaag gcgcagaagc caggcattct
      961 cacgcgcctg ctgcgtgtct ttttctgacg aggtaacgta tgcgaatcat cctcttgatg
     1021 ctggctcttt gcaccatagc gagcggattg gctgcccagc cgcagagccc ccaggccgcg
     1081 gcccccacgg ccccgcgccc ggctatccag acgcggatca aggagctggc gcgcatcgcg
     1141 ggggtacgga acaaccagct cttcggctac ggcctggtgg tgggtctgga ttcctcgggt
     1201 gacagcaacc aggtcgcctt cacggcgcgc gcggtagcca acatgatgca gcgcttcgga
     1261 atcaccctct ccagcgagca actcaagacg cgcaacgtgg ctgcggtgat cgtcacggct
     1321 gacctgccag cgttcacgcg cgagggcgac cgcatcgacg tgcttgtctc ctcggtaggg
     1381 gatgcgcgct cgctgcaagg aggcacgctg ctccagacgc cgctgcaagg cgcggacggc
     1441 gaggtgtacg ccgttgccca aggtccggtt tcgattggtg gctttgccgc cgggggcccg
     1501 ggggcaaaca tcagccgcaa ccaccccacc gcggggcgca ttcccaatgg cgccatcgtc
     1561 gagcgcgagg taccagtcac cgccgttgaa gatggattcc tgtcgctgcg cctgatgcgc
     1621 ccggacttct ctacagcggc acgcatggtg gatgcgatca atcgccggtt gggcagcggc
     1681 accgccactc cagtggacgc gggagcgatc cgcgtgcgca tcccctccgg gcaggagcgc
     1741 aatctggtcg ccctcgtcgc ccaactcgaa gcgattcctg tggagccgga catgccggcc
     1801 cgggtcatcg tcaacgagcg tacgggcacg gtggtcatcg gcgggcacgc ggtcatcgcg
     1861 cccgtcgccg tttctcatgg cagtctggtg gtggagatcg cccaggagcc ggtgatcagc
     1921 cagccctccc cactgggcga gggcgagacg gtcgtcacgc agaaagagca gacgcgcgtg
     1981 caggaggagg aagcgaccgt tttcccgctt caggccgcca cggtcgagga tctggtcaag
     2041 gcgctgaacg cgctgcgcct gacgccgcgc gacatcatcg ccatccttca ggcgatcaaa
     2101 caggcggggg cgcttcaggc cgagttggag gtgatctgat gcttcagcgg ataggggctc
     2161 ttgtggcagg gcaggcaggc gcgaccggga atgggcaaca gaaggcgctc gacaaggctt
     2221 gcgaggagat ggaggcgctc tttctgaagc agcttctctc cgagatgcgc cgggcgagtg
     2281 gaaccaccct gcgcggccct ggcggcgaga tctatcagga gcttttcgat caggagctgg
     2341 cccgacacct cgcgggcgcc agcgatctcg gcctggcacg gatggtgcaa cagcagctcc
     2401 agcgctccgc ggcgccggcc tcgccccaac agatccccgg tggggccggc gccgtggagc
     2461 caaaccgctc tcgccccatt taagcccata cgcagagatg ccgataggag gtaaggagcg
     2521 gcagcggaga ctttggctgt cgcggagagg gagatctatc gatgcgaata cagggcagag
     2581 aggcggtgct gcaggcgtat gtctctcggg tgtcggagaa gcgcgctggt caggtggatc
     2641 gcagcacgcc aaccatcgag caggaggacc gcgtcacgct ctcccgctcc gcccaggacg
     2701 tccggagggt gaaggagcac ctcgcgaacc tcccggaggt gcgggagaat cgcgtgaggg
     2761 agctacgtca gcttgtcgag cgcggcgaat accacgtatc cgaggaggag ctggcagatc
     2821 gcattttgga gtcgggcgtg cttgatttgc ccgttgattt cctgatctga ggaggccttt
     2881 gccatgcgta ttcagacgac gcgcttcggg cctctggacg tggaggcctc ctccatcttg
     2941 cggatccacc ctggcctgct ggggttcgag acacgccacc ggtatgtgct gctgcagacc
     3001 gaggagaacc ccctcttcca gtggctgcag tgcgtggatg acccggcgct cgccttcgtc
     3061 gtggtgg
//
LOCUS       DAOSCV010000544         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20559_length_3063_cov_3.150931, whole genome shotgun sequence.
ACCESSION   DAOSCV010000544 DAOSCV010000000
VERSION     DAOSCV010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20559_length_3063_cov_3.150931"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PLY56_12960"
     CDS             <1..328
                     /locus_tag="PLY56_12960"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015664.1"
                     /note="Catalyzes the formylation of AICAR with
                     10-formyl-tetrahydrofolate to yield FAICAR and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HOJ22434.1"
                     /translation="VEKTNVVDQFLLWDELSDAEKDALRAALTEEPQPLTPEERAEWI
                     ARFEGVALSSDAFIPFRDTIDRAHRSGVRYVLQAGGSVRDDEVTAAADSYGMVMIHSG
                     MRWFLH"
     gene            complement(387..2276)
                     /locus_tag="PLY56_12965"
     CDS             complement(387..2276)
                     /locus_tag="PLY56_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOJ22435.1"
                     /translation="MNSLWLVIGALAAFAVAYRYYGAFLAAKVAVLNDERATPAHRLR
                     DGVDYHPTNKLVLFGHHFAAIAGPGPLVGPVLAAQWGYLPGYVWIIIGACLAGAVHDF
                     VVLWASVREDGLSLPKIAQKSIGGFSGLTASLATLFIVIVTLASVAIVVVNALSESSW
                     GVFTIVVTIIAALITGAWMYRIRPGKVGEASAIGVAIVLIGVVLGRPFADSAFGPMLL
                     FSKPALSLILPSYALIASILPVWVLMCPRDYLSSYMKIGVMVVLGVGIFLAHPVLKMP
                     ATTPFVSGNGPVITGHLWPFLCITIMCGAISGFHALISSGTTPKMIYRERDILPIGYG
                     AMLVEGFIAVTALVAACALGPGDYFAINVSQDTPAQQAAYAQVVETARVEHGWDLTPT
                     ELPELEEGTGTKLAGRTGGAVTLAVGMAKVFASFPGMRELMSYWYHFVIMFEALFILT
                     LLETGTRVARFVFQEAASYFHPKATLGGKPNWGMNVLMSVVVCFAWGYLLYTGNINTL
                     WRMLGIVNQLLAAIALAVGTTYILMHSAKRRYALCTGVPFAFVIVTTVTASWLSIQGW
                     WAKIPAAAPAEAFSLRLMCILASIMLVLTIAIVADAVRRWVQILSRPAGAPEGERELE
                     TAGRA"
     gene            2547..2795
                     /locus_tag="PLY56_12970"
     CDS             2547..2795
                     /locus_tag="PLY56_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22436.1"
                     /translation="MDETLKLFDVKAQPARRAPDGIAYVCPRCWQRVTLLFAPEEPPL
                     CDPCSERMIAIPRGEEHTATTSSPEGPAGRQESGVMAG"
     gene            complement(2821..>3063)
                     /locus_tag="PLY56_12975"
     CDS             complement(2821..>3063)
                     /locus_tag="PLY56_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22437.1"
                     /translation="LPLGMLLGMPFPSGIRWAERDGSDQVPWLWGINGFASVLGSVLA
                     MVLAWKSGFSAALLAGMGSYALVALLAGMAHLTRKA"
BASE COUNT          621 a         1023 c          984 g          435 t
ORIGIN      
        1 cgtcgagaag acgaatgtcg tggaccagtt cctgctgtgg gacgagctct ccgatgccga
       61 gaaggacgcc ctgcgcgcgg cgctcacgga ggagccccag ccgctcacac ccgaagagcg
      121 cgccgaatgg atagcgcgtt tcgagggcgt tgcccttagt tccgatgcgt tcatcccgtt
      181 ccgcgacacg atcgaccggg cccaccgcag cggcgtccgc tatgtcctcc aggccggcgg
      241 ttctgtccgc gatgacgagg tgaccgccgc cgcggacagc tacggcatgg tgatgatcca
      301 ctctggcatg cgctggttcc tccactgacg aggggcgggg tgcccccgcc ccactccctg
      361 gatcgatgcg cgccggccgg ccgcggctac gcccggccgg cggtctccag ctctcgctca
      421 ccttctggcg cgccagccgg ccgcgagagg atctgaaccc agcgccgaac tgcgtctgcc
      481 acgatcgcga tggtgagcac gagcatgatg gacgccagga tgcacatcag gcgcagcgag
      541 aacgcctcgg ctggcgccgc agcggggatc ttggcccacc agccctggat gctcagccag
      601 cttgccgtca ccgttgtgac gatcacgaac gcgaacggca cccccgtaca cagcgcatag
      661 cgccgcttcg cagagtgcat caggatgtag gtagtgccca cggccagggc aatcgcggcg
      721 aggagctggt tgacgatccc gagcatgcgc cacagcgtgt tgatgttccc cgtatagagc
      781 aggtagcccc aggcaaagca gaccaccacg ctcatcagca cgttcatccc ccagttcggc
      841 ttgccgccaa gcgtcgcctt cggatggaag taggacgcgg cttcctggaa gacgaagcgc
      901 gccacgcgcg tgcccgtctc caacagcgtg aggatgaaga gcgcctcaaa catgatcacg
      961 aagtgatacc agtaggacat caactcgcgc atccctggga aggaagcgaa caccttcgcc
     1021 atgccgacgg cgagcgtcac cgcgccgccc gtgcgcccgg ccagcttcgt gcccgtgccc
     1081 tcttccagct cgggcagctc ggtcggggtc aggtcccacc cgtgctcgac gcgcgcggtt
     1141 tcgaccactt gcgcgtaggc ggcctgttgc gcgggagtat cctggctcac gttgatggcg
     1201 aagtagtcgc cgggccccag cgcgcacgcc gcaacgagcg cggtcacggc gataaagccc
     1261 tcgacgagca tggcgccata gccgatgggg agaatgtcac gttctcggta gatcatcttc
     1321 ggtgtagtgc ccgaggagat gagcgcgtgg aacccggaga tcgccccgca catgatggtg
     1381 atgcagagga agggccagag atggccggtg atgaccggcc cgttgccaga aacgaagggc
     1441 gtggtcgcgg gcatcttcag taccgggtgc gccaagaaga tgccgacgcc cagcaccacc
     1501 atcacgccga tcttcatgta ggagctgagg tagtcgcgcg ggcacatcag cacccacacc
     1561 ggaaggatgg aggcaatgag cgcgtaggac gggaggatga gcgagagcgc cggcttgctg
     1621 aagaggagca tcggaccgaa cgcggaatcg gcgaagggcc ggcccagcac gacgccaatc
     1681 agcacgatcg ccacgccaat ggcggaagcc tctcccacct tccccggtcg aatccgatac
     1741 atccaggcgc ccgtgatcag tgccgcgatg atcgtgacta cgatggtgaa gacgccccag
     1801 gagctttcgc tcaacgcatt cacgacgacg atggccacgc ttgccagggt gacgatgacg
     1861 atgaagagcg tcgccagtga ggccgtcaac cctgagaaac cgccgatact cttctgggca
     1921 atcttcggaa gcgaaagccc atcctcgcgc accgaggccc acaggacgac aaaatcgtgc
     1981 acggcgccag ccaggcaggc gccgataatg atccagacat aaccgggaag gtagccccac
     2041 tgcgccgcga ggaccggacc aaccagcggc cccggaccgg caatcgccgc gaagtggtgg
     2101 ccgaagagga ccagtttatt cgtggggtgg taatccaccc catctctcaa ccgatgtgcc
     2161 ggcgtcgccc gctcatcgtt gaggacagcg accttggcgg ccaggaacgc gccatagtag
     2221 cggtaggcaa cagcgaacgc ggcgagggca ccaatgacga gccacaagga attcatcgat
     2281 tcgcacctct ctgtcgggag caagcaatca gggggcaggc gcccaggaga gacaggtgct
     2341 gccggagagc ggtcgcgggc ggagacgcga ccctacgagt cacgccgatg gctgtagggg
     2401 ccggcctctg ttgccggttc ggctcagctc ccgagtctag ggaaaaatac gacgcccggc
     2461 cggcgatgcc cttctcggaa aatgcaccct gcgccaggca ggcacgccac aggcgcatgt
     2521 cgaaaaagag gtcggaagga gaacgcgtgg acgagacact caagctattt gacgtgaaag
     2581 cacagccggc aaggagggcg ccagacggta tcgcgtacgt ctgcccccgg tgctggcagc
     2641 gcgtgaccct tctcttcgct cccgaggagc cccctctctg cgacccctgc tccgagcgga
     2701 tgatcgccat tccacgcggt gaggagcaca ccgccaccac ttcatccccg gaaggccccg
     2761 ccgggcgcca ggaatcaggc gtcatggcag gctgaggaga gccaccgcag acgcccggct
     2821 tcacgctttg cgcgtcagat gcgccatccc ggccaggagg gccaccagcg catagctccc
     2881 catcccggcc aggagcgcgg cactgaagcc cgacttccat gccagcacca tcgcgagcac
     2941 ggagcccagc accgaagcga agccgttgat cccccatagc cacggcacct ggtcggaacc
     3001 gtcgcgctcg gcccagcgaa tgcccgaagg gaacggcatc cccaacagca tccccagggg
     3061 aag
//
LOCUS       DAOSCV010000545         3059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20593_length_3059_cov_3.709055, whole genome shotgun sequence.
ACCESSION   DAOSCV010000545 DAOSCV010000000
VERSION     DAOSCV010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20593_length_3059_cov_3.709055"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PLY56_12980"
     CDS             complement(<1..297)
                     /locus_tag="PLY56_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOJ22438.1"
                     /translation="MATRRIAIDAHQPDPAVIAEAAAVLRAGGLVAFPTETVYGLGAP
                     AMDEAAVRRVFEAKGRPENKPLIIHLSRVEQVERVARHVPDLAWGLTRRFFPGPL"
     gene            complement(279..887)
                     /locus_tag="PLY56_12985"
     CDS             complement(279..887)
                     /locus_tag="PLY56_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOJ22439.1"
                     /translation="MPRLCILLLVPVGIWLFWQPDTLRLFLSRRPESIIIHHSASPPR
                     VHGRPVDAAAIDAMHARRGFGIVHEGKVYHIGYHYVVLPDGTVQAGRPEECVGAHARH
                     VNEHSLGICLVGNFHSAENPSGAQGPVRPTPAQMDALIELCARLCRTYRIPVERVCRH
                     RDVGETACPGDRFPFETFQSHLRERVERGGRSIKDLLWQRGE"
     gene            complement(1235..2752)
                     /locus_tag="PLY56_12990"
     CDS             complement(1235..2752)
                     /locus_tag="PLY56_12990"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ22440.1"
                     /translation="MAEVAEQEAAGSNRRIWIVVGIVVLIIAYLVARSVQKKMIISGL
                     RSEENSVRVTSAQKLMEMKILTDVLSRQPVPVKRPMIRTLRDMNTEAAADELIALMKD
                     RNPTVQNEAQFALQRMAPTSIGKLVGALSNPDLNVKNRAAMALIAIGSSVLTPVEKDG
                     KQSVPLIDAIATPAARPGVTRVVTTLRGDVAPWLLQKLADESTDPGTKACAIDILGRL
                     GVQEAAKPIRAMLDAAEVPVPIRNAALVALGRLRHPDAYALLNAAIADKDPALVSVAI
                     QGFGELQNPAAVSLLQKYLFGYNEALRDAAVAALSKIGAPAVPALAGLVKSPRYEVRI
                     GALRALEGIDDPRTPGIIASALKDPDENVRRMAAEALGRPGDARGIAPLIAALSDPKW
                     RVAARARDSLAEIGSPAVPALVATLSNAAQSSASFYAQQALTRIGTSAVPALMSAART
                     GNVEARQRAVLVLGAIGDSRAVDLLKSLSTSDSIPAIRQAAERALKQVQEGRRIG"
BASE COUNT          541 a         1068 c          947 g          503 t
ORIGIN      
        1 cagtgggcct gggaagaagc gcctcgtcag cccccatgcc agatcgggaa catgccgcgc
       61 cacgcgctcc acctgctcca cccgagacag gtggatgatc agcggcttgt tctccggccg
      121 ccctttcgcc tcgaagaccc ggcgcacagc ggcttcatcc atcgctggcg cgcccaggcc
      181 atacaccgtc tcggtcggga aggcgaccag cccacccgcc cgcagaacgg cagctgcttc
      241 cgcgataacc gccgggtcgg gctgatgcgc atcgattgct attctccgcg ttgccataac
      301 agatccttga tggaccggcc tccgcgctcc acgcgttcac gcaggtgaga ctggaatgtc
      361 tcgaagggaa accggtctcc agggcacgcc gtctcgccaa catcccggtg ccggcatacc
      421 ctctccaccg ggatccggta ggtgcggcat aagcgggcgc aaagctctat cagcgcgtcc
      481 atctgtgccg gcgttggccg caccgggccc tgcgctcccg aagggttttc cgcactgtgg
      541 aagttgccca ccaggcagat gcccagggaa tgctcgttca cgtgccgggc gtgcgcgcca
      601 acgcactctt cgggccgccc tgcctgaacc gtgccatcgg gcagaaccac atagtgatag
      661 ccaatgtggt atactttccc ctcatgcaca ataccgaacc cgcggcgggc gtgcatggca
      721 tcaatcgctg ccgcatccac cggccgcccg tggacacgcg gcgggctggc agagtgatgg
      781 atgatgatgc tttcgggcct ccgagagagg aaaaggcgca gcgtatccgg ctgccagaaa
      841 agccagatcc cgaccggcac caggagcagg atacacaacc ggggcacgcc tctgcgccat
      901 gagccatgcc gacgcgcccg gcgagaaggg gccttccggg accggtatcg gtcgcgagat
      961 gttgagcgcg gtcgcatccc cagcgaggcc agagtccttc cgtcccaggt gaaacgcacg
     1021 acggtgcgag cgaaggcaga acgcaggagg cattctacgt gctccccagt ctgccggaaa
     1081 gttcgaaaac cggcgaggga gagacggacc agaggcccgc gcttccctcg ccggtcatat
     1141 catcaacacg cagcgtcgcc ttcctcggcc ggcaccacgc gctccccatc cgccacacga
     1201 gagacggctc gcgtggccag gcggcaggct accctcagcc gatgcgacgg ccctcttgta
     1261 cctgcttcaa ggcacgctca gcggcttgcc gaatcgccgg aatgctatca ctcgtgctga
     1321 gcgacttgag caggtcaacg gcgcggctgt cgccaatcgc ccccaggacg aggaccgcgc
     1381 gctgtcgggc ctccacattt ccggtgcgag cggccgacat gagggcgggc actgcgctgg
     1441 tgccaatgcg cgtgagcgcc tgctgcgcgt agaaggacgc ggaagactga gcggcgttgc
     1501 tcagcgtcgc gacgagggcc ggcaccgccg gcgagccgat ttcggcaagc gagtcgcgtg
     1561 cgcgcgccgc cacgcgccac ttcgggtcgc tcagcgcggc gatgagcggc gcgataccgc
     1621 gggcatcgcc cgggcggccc agcgcctccg ccgccatgcg gcgcacgttc tcatccgggt
     1681 cctttagcgc cgaagcgatg attccaggag ttcgcgggtc gtcgattccc tccaacgcac
     1741 gcaacgctcc gatgcgcacc tcgtagcgcg ggctcttcac caggccggcc agcgcgggca
     1801 ctgcaggagc gccgatcttg gagagggcgg ccaccgcggc atcgcgcaat gcctcgttgt
     1861 agccaaagag gtatttctgc agcagcgaga cagccgcggg gttctgcagc tcgccgaaac
     1921 cctggatggc cacgctcacc agagcgggat ccttatccgc gatagcggcg ttcagcaaag
     1981 cataggcatc cggatggcgc agccggccca gggctaccag ggcggcgttt cggataggca
     2041 caggcacctc ggcggcatcc agcatcgccc ggatcggctt tgctgcttcc tgaacgccca
     2101 ggcggccgag gatatcgatg gcgcacgcct tcgtgccagg atccgtgctc tcatcggcaa
     2161 gcttctgcaa caaccacggc gccacgtcgc ctcgcagcgt ggtcactacc cgcgtgacgc
     2221 ccgggcgcgc ggcaggagtg gcgatcgcgt cgatgagagg cacgctctgc ttgccgtctt
     2281 tctcgacagg cgtgagcacc gaactgccga tggcaatcag cgccatcgcc gcgcggttct
     2341 tcacattcag atccgggttg ctcaacgcac cgaccagctt cccgatcgac gtcggcgcca
     2401 ttcgctgcaa ggcaaactgt gcctcgtttt gaaccgtcgg gttgcggtcc ttcatgagcg
     2461 cgatcagctc gtcagcggcc gcttccgtgt tcatatcgcg cagggtgcgt atcattggcc
     2521 gcttgaccgg gacaggctgc cggctgagga catccgtgag gatcttcatc tccatcagct
     2581 tctgcgcgct tgtcacccgc accgagttct cctcgctgcg aagcccagag atgatcatct
     2641 tcttctggac gctacgcgcc accagatacg cgatgatgag gacgacaatg ccgacgacga
     2701 tccaaatacg gcgattgcta ccggcggctt cctgctcggc tacttctgcc actgttgacc
     2761 tcctcggatc gtgggggact gataccgcgg tcggttcgcg atggcaccac tctcgcgcct
     2821 gcccggggcc tcgtatccag gggaaccgag gcgcgagcac ggccatcccc gactgaaacc
     2881 ggcacaaact gacggtgcca ttatcccaca cggcaggggc tttgtcaacc ctgtgcctta
     2941 tgcgcaaaag gccaggcgcg caccgaacac gcccctcata aacgcgcccc tggccaagag
     3001 ggctctcccc cgcaacacgc ccttattcga cgtggcccac gctcctccct tcccctcgg
//
LOCUS       DAOSCV010000546         3052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20654_length_3052_cov_2.965299, whole genome shotgun sequence.
ACCESSION   DAOSCV010000546 DAOSCV010000000
VERSION     DAOSCV010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20654_length_3052_cov_2.965299"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="PLY56_12995"
     CDS             complement(<1..1414)
                     /locus_tag="PLY56_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ22441.1"
                     /translation="MRAPWDGRLACVRRRLGVVVLFLAWMACVVASASPTLAREQRRV
                     RLTVWGWWNREGYKQAAAEFERRHPHIEIVTSTAGGQMDEQKLMTAIAGGSPPDVINQ
                     DRFSVGGWAARDAFLPLDEFIARDGAIRAEDYYPACWNEAVYEGRVYAIPNSTDDRLL
                     FYNEDLLRQAGFVDAAGEVIRPRTWHELKHYAIKMTRRDARGNLTRVGFVPNYGNSWL
                     YLYGWQNGGRFLSEDGRTCLLNEPAIVGALEWVTGVYDALGGATAVDAFTATFQGGEL
                     DPLLTGKVAMKIDGNAVLNFIASTRPDFNFGVAPPPVPEGREPLTWSGGFSWAIPRGA
                     KHPEEAWEFIKWMSSVEAGLLIADAEYRWNRSWGRLYVPEISPNKRVNEAVLQRYAPR
                     HPRFARAFRLALEMMNRSRYRPVTPVGQVLWDEHVRAMDLAIHKKLTPRQALDAGTRA
                     VQKALDRIHKRKPYPPLDPRV"
     gene            complement(1494..2165)
                     /locus_tag="PLY56_13000"
     CDS             complement(1494..2165)
                     /locus_tag="PLY56_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOJ22442.1"
                     /translation="MRREKGAPEPISALNRVPLRECGEPLVDIRRYCPGVRISRKAIP
                     FLREQVAEMLRAAQAALPAGYRLWVRTALRTLEMQREMYDRNFERIRQEHPTWSYATL
                     RRATNRFFAPVDQKAPPGHCTGAAVDVYLLTPSGHLADLRSPFTGWEGAPTWIEGLRP
                     SAQRNRGILLDAMLGAGFSNCRDEFWHFSYGDAAWAVRTGQDHCIYGLVEPPPGWPRK
                     PKPRA"
     gene            2479..2985
                     /locus_tag="PLY56_13005"
     CDS             2479..2985
                     /locus_tag="PLY56_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ22443.1"
                     /translation="MPAAPEDAEVKEILKILERDSRVTPEQIAEMTGRDAGEVARLIA
                     EAEAEGIIRRYKSVIDWERAGNERVLAFIDVRVLPEPNHGFDDIARRIYRHPEVRSVY
                     LVSGQNDLRVIVEGRTMREVAFFVAERLATIEQVQATTTNFILKRYKEDGDIFEDQEP
                     DRRLAVAP"
BASE COUNT          515 a         1008 c         1010 g          519 t
ORIGIN      
        1 gaacgcgcgg atccagcggc gggtagggct tacgcttgtg gatgcggtcg agcgccttct
       61 ggacggcgcg ggtgcccgcg tcgagtgcct gcctgggcgt gagcttcttg tggatcgcca
      121 gatccatggc gcgcacgtgc tcgtcccaca gcacctggcc gacgggcgtg accggccggt
      181 agcgcgaccg gttcatcatc tcgagcgcga ggcgaaacgc gcgcgcgaac cgtggatggc
      241 gcggggcata ccgctggagg accgcttcgt tgacgcgctt attcggcgag atctcgggca
      301 cgtagagccg gccccaggag cggttccagc gatactccgc gtccgcgatc aggaggccgg
      361 cctccacgct ggacatccac ttgataaact cccaggcctc ttccggatgc ttcgcgccgc
      421 ggggaatcgc ccacgagaat ccgccggacc aggtgagcgg ctcgcggccc tcgggtaccg
      481 gcggcggggc cactccgaag ttgaagtccg ggcgcgtact cgcgatgaag ttgaggaccg
      541 cgttgccgtc gatcttcatc gccaccttgc cggtgaggag cgggtcgagc tcgccgccct
      601 ggaaagttgc cgtaaaggcg tcgaccgcgg tcgctccgcc cagggcatcg tacactccag
      661 tcacccactc cagggcgccg acaatggccg gctcgttcag gagacaggtc cggccatcct
      721 cgctcaggaa acgcccgccg ttctgccatc cgtacagata gagccacgag ttgccatagt
      781 tgggcacgaa acctacccgc gtcaggttgc cccgggcatc gcgccgggtc atcttgatgg
      841 cgtagtgctt cagctcgtgc caggtgcgtg ggcggatcac ctcgccggca gcgtcgacaa
      901 agccggcctg gcgcaacaga tcctcgttgt agaaaaggag acggtcgtcc gtggagttgg
      961 ggatggcata gaccctcccc tcgtaaaccg cttcgttcca gcaggcagga tagtagtcct
     1021 cggcgcggat cgctccgtcg cgggcgatga actcatcgag gggcaggaag gcatcgcgcg
     1081 cagcccagcc gcccacgctg aagcggtcct ggttgatcac gtcgggcggg gagcctccgg
     1141 cgatggcggt catcagcttc tgctcgtcca tctgcccgcc ggcggtggac gtgacgatct
     1201 cgatatgcgg gtggcggcgc tcaaactcag cggctgcctg cttatagccc tcgcggttcc
     1261 accagcccca cacggtcagc cggacgcggc gctgttcccg cgccagcgtg gggcttgcgc
     1321 tggctaccac gcacgccatc catgccagga agaggaccac taccccgagg cggcggcgca
     1381 cgcacgcaag acgaccatcc caaggcgctc tcatcctgcc tgtagctatg gcacaatccg
     1441 cgccgaagtg ccgggcgcct cctgccgcgg gcgcgcgccc gcccggccgg tgcctacgcg
     1501 cgcggctttg gcttcctcgg ccaccccggc ggcggttcca ccaatccata aatgcagtga
     1561 tcctggccgg tccgcactgc ccatgctgcg tcgccatacg agaagtgcca gaactcatcg
     1621 cggcagttgc taaagccggc gccgagcata gcgtccagaa ggatgcctcg attgcgttgc
     1681 gctgagggac gcagaccctc gatccaggtg ggcgcccctt cccaaccggt gaagggagag
     1741 cggaggtccg ccagatggcc gctgggcgtc agcaggtaga catccaccgc ggcgcccgtg
     1801 cagtgtcccg gaggcgcctt ctggtccacc ggcgcgaaga agcggttggt cgctcgacga
     1861 agggtggcat agctccaggt ggggtgctcc tgacggatgc gctcgaagtt gcgatcgtac
     1921 atctcgcgct gcatctctag cgttcgcagc gctgtgcgca cccacaggcg atagccggcc
     1981 gggagcgccg cctgcgcggc acgaagcatc tctgctacct gctcgcggag gaaggggatc
     2041 gccttccgcg aaatacgcac ccccgggcag tatcgccgga tgtccaccag cggctcgccg
     2101 cactcgcgca gcggcacgcg gtttagggcc gagatcggct ctggagctcc tttctcacga
     2161 cgcatcgttc acccttcctg atagctctta ccgtcgtttc tcatcatact cgccggctga
     2221 caagccgtca agcggggccg aggctctccg ccggccccgc catgcggcaa agcggcgctc
     2281 gtgtgcgggc cgcattctgt ttgcgtctca cgcttcgagt gtgctataat gagcgtcaga
     2341 gctcgcctgg gtgtttggcg acgcctcagg acaggcgccg ctgccatgcc gtccaaccgg
     2401 ctgctttcag gcgtgcactc ctgccaatta cagggccgcc tgcgcggccc tgggtcgttt
     2461 agctgccgcg agggcggcat gccggcggcc ccggaggacg cggaagtgaa ggagatcctc
     2521 aagattctgg agcgtgactc gcgagtcacg cccgagcaga ttgccgagat gacggggcgc
     2581 gatgcgggcg aagtcgcacg cctgattgcg gaagcagagg ccgagggcat cattcgccgc
     2641 tacaagagcg tcatcgattg ggaacgcgcc gggaatgagc gcgtcctggc gttcattgac
     2701 gtgcgcgtgc tcccggagcc caatcatggg ttcgacgaca ttgcccgacg catctaccgc
     2761 catcccgagg tgcgctcggt ctacctggtc tccggccaga atgacctgcg cgtcatcgtc
     2821 gaagggcgaa cgatgcgaga agtggcgttt ttcgtggcgg agcgattggc aaccatcgag
     2881 caggtgcagg caactacgac gaatttcata ctcaagcgct ataaggaaga tggcgacatc
     2941 ttcgaagacc aggagcccga ccgtcgtctg gcggtggccc cctagctccg ggagggaatt
     3001 cagatgaaag acacgcgaca gaccgtggga tccacccttg tgcttcccgc tg
//
LOCUS       DAOSCV010000547         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20667_length_3051_cov_3.525701, whole genome shotgun sequence.
ACCESSION   DAOSCV010000547 DAOSCV010000000
VERSION     DAOSCV010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20667_length_3051_cov_3.525701"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /gene="metK"
                     /locus_tag="PLY56_13010"
     CDS             <1..834
                     /gene="metK"
                     /locus_tag="PLY56_13010"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ22444.1"
                     /translation="MGVDRGGAGDQGMMFGYATNETQELMPMPLMLAHKLARQLSLVR
                     RNGTIPYLRPDGKTQVTVEYENGRPIRVDKILVSSQHDPSVKQSTIYEDIVEHVIKPV
                     IPEHMVDSQTKPPLVNPTGSFAVGGPQGDTGLTGRKIIVDTYGGMARHGGGAFSGKDP
                     TKVDRSAAYMMRYIARQIVGAGLADRCELQVAYAIGEREPLSVNINTFGTGQIPDGQI
                     LQIVSRLFDLTPGAIIEKLQLRRPIYRQVAAFGHFGRTDLDVAWEKDDMVDAVRNAAG
                     V"
     gene            1143..>3051
                     /gene="priA"
                     /locus_tag="PLY56_13015"
     CDS             1143..>3051
                     /gene="priA"
                     /locus_tag="PLY56_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOJ22445.1"
                     /translation="MFANVVVDLEGRGLPGPLTYAIPEAMRPLVRIGTYVQVPLGTRQ
                     ALGYVVELQGEFEGTAKPLAAVLYSEPLFDPPLLRLAIWMAHRYRASLAETLRCIVPE
                     GIATRLRTRVALEGVADPEDAARTLERRSPALASLLRILAQEGAIDTRTLRERWKGSN
                     LTAALGRLRTRGWIRQEMALESPPARPREVTVFRPAVGTEALAAEAEARRQRAPAQAQ
                     ALAHFLESGAGGFTAAELAQRGTSRSVLQALARDGVLVAERVSQRRNPWSGRATATAP
                     PPLTPDQSAAAAAIAETIHAGQHDTILLHGVTASGKTEVYLRAIETALSANKGAIVLL
                     PEIALTAQVVEVFKSRLGERVALLHSRLSAGERYDEWQRLRTGEARVAVGPRSALFAP
                     IANLGLVILDEEHEPSYKQENSPRYHARYAAIQRARDADAALVLGSATPSVESYYHAC
                     NEDYRLVRMPERIPGRAMPSVRVVDMRAHPPLGPEGVFSDELLDALARRVHDGEQAVL
                     FLNRRGFASFILCRDCGYTSRCPHCSVSLTLHASDASLRCHHCNHQRPAPSLCPVCKG
                     ERVLPFGLGTERVEAAVHAHLPEARVLRMDRDTTGGKNAHARFYRAFRAGEAEILIGT
                     QMVAKGLDFPGV"
BASE COUNT          487 a         1005 c         1044 g          515 t
ORIGIN      
        1 atgggcgtgg accgcggcgg ggccggcgac caggggatga tgttcggata cgccaccaac
       61 gagacgcagg agctgatgcc gatgccgctc atgctggcgc ataagctggc ccggcaactc
      121 tctctcgtcc gccgcaacgg cacgatcccg tacctgcgcc cggacggcaa gacccaggtc
      181 acggtcgaat acgagaatgg acgcccgatc cgggtggaca agattctcgt ctcctcgcag
      241 cacgatcctt ccgtcaagca gagcaccatc tacgaggata tcgtcgagca cgtcatcaag
      301 ccggtgatcc ccgagcacat ggtcgactct cagacgaagc cgcccctggt caacccgacg
      361 ggcagctttg ctgtgggggg cccgcagggg gataccggcc tcacaggacg caaaatcatc
      421 gtcgatacct atgggggcat ggcacgccac ggcggcgggg ccttctccgg caaggacccc
      481 accaaggtgg accgctcggc ggcctacatg atgcgctaca tcgcccgcca gattgtcggc
      541 gccggcttgg ccgaccgctg cgagcttcag gtggcctacg ccattggcga gcgcgagcca
      601 ctctccgtga atatcaacac cttcgggacc ggccagatcc ccgatggtca gattctgcag
      661 attgtgagcc gcctcttcga cctgacgcca ggcgcgatca tcgagaagct gcagcttcgc
      721 cgtccgatct accgccaggt ggctgccttt ggccactttg ggcgcacgga tctcgacgtg
      781 gcctgggaga aggacgacat ggtcgacgcg gtgcgcaacg ctgcaggcgt ctgatctctt
      841 ccgcgctggt ctgatccagc cgggtggcaa gcacgatagc ggcgcagagg acacaggagc
      901 gcatcgagca ctgtgtcctc tgtgacgtac tcctcccgcg tctgagccgg gcacttgccc
      961 gatgcgacac caatggctct ccccttccga tgggtctcgc ttgaatgggc cgcgggcact
     1021 cccaccggcc atcgcgcgat ccgccgcggg gtttgatact cgcggggcag gggagagtgg
     1081 accgcgcggc cggctgagcc atccggggta gacccgacgc atcacgaaaa gagccgagct
     1141 tcatgtttgc aaacgtggtc gtcgatctcg agggccgcgg actgccgggg ccactcacct
     1201 acgcgatccc agaagcgatg cgcccgctgg tgcgcatcgg gacgtacgtc caggtgcccc
     1261 tgggcacgcg gcaggcgctc ggctacgttg tggagttgca gggcgaattc gagggcactg
     1321 cgaagcccct cgccgcggtg ctctattcgg agccgctttt tgatccgccg ctattgcgcc
     1381 tcgctatatg gatggcccat cgctatcgtg cctctctcgc ggagacgctg cgctgtattg
     1441 ttcccgaggg aattgccacc cgcctgcgca ctcgggtggc tctggaaggc gtggccgatc
     1501 cggaggatgc cgcccggacg ctggagcggc ggtcgccggc gttggcatcc ctcctgcgga
     1561 ttctggcgca ggagggagcg atagataccc gaacgctgcg cgagcgctgg aaggggagca
     1621 atctgaccgc cgcgctgggc cggttgcgca cccgggggtg gattcgtcag gagatggcgc
     1681 tggagtcgcc accggcgcgc ccgcgtgagg tgacggtgtt tcggccggcg gtcggcaccg
     1741 aggccctcgc tgccgaggcc gaggcgcgcc gccagcgcgc accggcccag gcgcaggcgc
     1801 tcgctcactt tttggagagc ggcgcgggcg gtttcaccgc ggccgagttg gcgcagcgcg
     1861 gcaccagtcg tagcgtgctt caggctctgg cacgggatgg tgttttggtc gccgagcgcg
     1921 tgagccagcg gcgcaatccg tggagcggac gggcaactgc taccgcccca ccgcccctta
     1981 ccccggatca gagcgcggcg gcggcggcga ttgcggagac aattcacgcc ggccagcatg
     2041 ataccatcct ccttcacggc gtgacggcga gcgggaagac ggaggtctat ctgcgcgcga
     2101 ttgagaccgc gctttccgcg aacaaaggcg ccattgtcct gctgccggag atcgcgctca
     2161 ccgcgcaggt ggtggaagtg ttcaagtcgc ggctgggaga gcgcgtggcg ttgttgcact
     2221 cgcggctttc cgccggggag cggtatgatg agtggcagcg gctgcgcacc ggcgaggcgc
     2281 gagtggccgt gggcccgcga tcggcgctct tcgcgcccat tgcgaacttg ggcctggtga
     2341 ttctggacga ggagcacgag ccatcctata agcaagagaa ctcgccgcgc taccatgcgc
     2401 gctacgccgc cattcagcgg gcccgcgacg cggatgccgc gctcgtcttg gggagcgcca
     2461 ccccatcggt ggagagctac taccacgcct gcaacgagga ttaccgtctg gtacgcatgc
     2521 cagagcgcat cccagggcgc gcgatgccct cggtgcgcgt cgttgatatg cgcgcgcatc
     2581 cgccgctggg gccggagggc gtcttcagtg acgagctgtt ggatgccctc gcccgacgcg
     2641 tgcacgatgg cgagcaggcg gtgctgttcc tgaaccggcg cggctttgcc agcttcatcc
     2701 tgtgtcgcga ctgcggatac acctcccggt gcccgcattg tagcgtttcg ctgactctcc
     2761 atgccagcga cgcgtcgctc cgctgccatc actgcaatca ccagcggcca gcgccctcgc
     2821 tctgtcccgt atgcaagggc gagcgcgtgc ttccgttcgg cttgggcacc gagcgcgtcg
     2881 aagcggcggt gcatgcccac ctgccagagg cgcgcgtcct gcgcatggat cgcgatacca
     2941 cgggcgggaa gaacgcgcac gcgcgattct accgggcgtt tcgcgccggc gaggccgaga
     3001 tcctgatcgg cacgcagatg gtcgccaagg ggctcgattt ccctggtgtc a
//
LOCUS       DAOSCV010000548         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20720_length_3046_cov_3.113006, whole genome shotgun sequence.
ACCESSION   DAOSCV010000548 DAOSCV010000000
VERSION     DAOSCV010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_3046_cov_3.113006"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLY56_13020"
     CDS             <1..425
                     /locus_tag="PLY56_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ22446.1"
                     /translation="DGVSIDQNIERLLGVVADLPWQARKAAQVEEDCRVHPEGVFVAV
                     VANDVAGYVTTRVNPSTRTGWIPHLAVDPRFRGRGIARALLAHAQDYLRERGMVTAKI
                     ETLEQNATAASLFPKLGFREIARQIHYIKPLGPEMEKE"
     gene            463..1281
                     /locus_tag="PLY56_13025"
     CDS             463..1281
                     /locus_tag="PLY56_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ22447.1"
                     /translation="MMSPQLPQGFAAVDGGEDPHAFVSYVDAVSALSSAQAYKQLTLL
                     MLRVGEGARVLDVGCGTGDDARALAQVVGPRGWVVGIDNSRTMIEEATRRQNGLGLPV
                     EFYVGDAYQIAFPDEVFDACRADRVFQHLREPKKALSEMIRVTRSGGRIVVSDPDWGT
                     FIVNSPDRDLTRRIVGFACDATTDGWMGRQLPGLFREVGLQDVQVRTSLVVIEEYDTA
                     KEVLGIEWLAHGAAQAGVITSGEAERWLRGLQEDHAAGRFTCGLGGFITSGVKP"
     gene            1381..2043
                     /locus_tag="PLY56_13030"
     CDS             1381..2043
                     /locus_tag="PLY56_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ22448.1"
                     /translation="MEATAVQASGRQVQVSGRDVTLEGFLAVPENAQGVVVFAHGSGS
                     GRFSPRNNYVAGVLQEGGLATLLIDLLEESEADDRDKVFDIDLLAERLRLATGWLHGE
                     PSTRHLRVGYFGASTGAAAALQAAALEGAEIDAVVSRGGRPDLAMPYLPRVQAPVLLI
                     VGGNDLPVIPLNEQAYAALETTKELVIIPGATHLFEEPGKLEEVACLARDWFQRYLVP
                     ME"
     gene            2403..>3046
                     /locus_tag="PLY56_13035"
     CDS             2403..>3046
                     /locus_tag="PLY56_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ22449.1"
                     /translation="MDSIAKEVIPVNIEDEMRESFLLYSMSAITQRAIPDVRDGLKPV
                     HRRILWTMHEDGMVPGGAWYKSARLVGDVMGKYHPHGDAAIYDTIVGMAQDFKSRYPL
                     VAGHGNFGSIYGDSAAAMRYTEVRMAPLAQEMVVDIGRDTVDMVPNYDNKQLEPAVLP
                     SRIPNLLINGSMGIAVVYTTNIPPHNLREVCDAALLYIDNPNATVDELMKVLPGP"
BASE COUNT          528 a          962 c         1010 g          546 t
ORIGIN      
        1 tcgacggtgt ctctatcgac cagaacatcg agcggctgct tggggtggtg gccgatcttc
       61 cctggcaggc gcgaaaggca gcgcaagtgg aggaggactg ccgggttcac cccgagggcg
      121 ttttcgtcgc ggtggtggcc aacgacgtgg cgggttatgt caccacccgg gtcaacccga
      181 gcacgcgcac agggtggatt ccccacctcg ccgtcgatcc gcgctttcgt ggacgaggga
      241 tcgcgcgcgc cctcctggcg catgctcagg actacctgcg cgagcgcggc atggtcaccg
      301 cgaagataga gacgctcgaa caaaatgcga ctgccgcgag cctcttcccc aagctggggt
      361 ttcgggagat tgcccggcag attcactaca tcaaacctct cgggccggag atggagaagg
      421 agtgagcaga tcccgagagg gctggaggat ggatccacta caatgatgtc tccgcaattg
      481 ccacaagggt tcgccgcggt cgatggagga gaagatccgc acgcttttgt cagctacgtg
      541 gatgccgtca gtgctctctc ctcagcgcaa gcgtataaac agctcactct cctcatgctg
      601 agggtgggcg aaggcgctcg cgtcctcgac gtcggctgcg gcaccgggga cgacgcccgc
      661 gccctggccc aggtagtggg cccgcgcggc tgggtggtcg ggatcgataa cagccgcacc
      721 atgatcgagg aagctacccg ccgccagaac ggccttgggt tgccggtcga gttctatgtc
      781 ggcgacgcct accagatcgc tttcccggat gaggttttcg atgcctgccg ggcggaccgc
      841 gtcttccagc acctgcgcga gccaaagaag gcgctctccg agatgatccg cgtgactcgc
      901 agcggtggcc gaattgtcgt cagcgatccc gactggggca cgttcatagt caacagcccc
      961 gaccgcgacc tcacgcgccg gattgtcggc tttgcctgcg atgcaaccac ggatggctgg
     1021 atgggccggc aacttccggg cctattccgg gaagtgggcc ttcaggatgt tcaggtccgc
     1081 acctccctcg tggtgatcga ggagtacgac acggcgaagg aggtgttggg gatcgagtgg
     1141 ctcgcgcacg gggccgcgca agccggcgtg attacgtccg gggaggcaga gcgctggctt
     1201 cgggggcttc aggaggatca cgccgccggc cggttcactt gcggcctcgg gggcttcatc
     1261 acgtctggag tcaagcccta gggccaggcc gctcgttccg gctacggcgt ggttccgttt
     1321 gcgattccgg tgggtagatc gaagagtacg cgagatctac cttatcggaa ggagaatggg
     1381 atggaagcga ccgcggtgca ggcgagcggc cggcaggttc aggtatcggg acgtgatgtc
     1441 accctggagg ggttcctggc ggtgccggag aatgcccaag gcgtcgtcgt attcgcccac
     1501 ggcagcggca gcgggcggtt tagccccagg aacaactacg tcgcgggggt cctccaggag
     1561 gggggcctgg ccacccttct gatcgatctc ctggaagaat cggaagcgga cgaccgcgac
     1621 aaggtctttg acattgacct gttggcagag cgcctgcgtc tggccaccgg ttggctgcat
     1681 ggcgagccgt ctacgcgcca ccttcgcgtg ggctacttcg gggcgagcac aggcgcagcc
     1741 gccgctctcc aggccgccgc gctcgagggc gcggagatcg acgccgtcgt ctcgcgtggc
     1801 ggccggcccg acctggcgat gccctatctc ccgcgcgttc aggcgccggt gctcctgatt
     1861 gtcggcggta acgatcttcc ggtgatcccg ctgaacgagc aggcttacgc ggcgcttgag
     1921 accacgaagg agctggtgat catccccgga gcgacgcatc tcttcgagga gcccggcaag
     1981 ctggaggagg tggcctgcct cgcccgcgac tggttccagc gctacctggt gccgatggag
     2041 taggccatcc catcgcgaca aacgagccgg ccacgcgcca gcgcgcttct gcgcgcaggg
     2101 agctcctccg gccttgcccg gaacgatttc gcgcccgcag cccggctgac acgagtgccc
     2161 ggcaacggcg ctgcctgccc ccatcccctg cttcgctctt ggtcgtgcct atctataggt
     2221 attcatgtgc ggtcactcct atttggatga acgcccgttc tccttgactg ggcgtgacaa
     2281 atatgctata ctgcaaatgt cagtttgcca ctatcacacg tggattttcc ggccgtggtt
     2341 ggtaccatcg ccttcgcaag cgcccacgcg cgtggcaaac gagccacttg tgaggaggtt
     2401 gcatggattc catcgccaag gaggtcatcc ccgtcaacat cgaggacgag atgcgggagt
     2461 cgttcctgct ctactcgatg agcgccatca cgcagcgcgc gatccccgat gttcgagacg
     2521 ggctcaaacc cgtgcaccgg cgcatcctgt ggacgatgca cgaggatggg atggtgccgg
     2581 gcggtgcctg gtataaatcc gcgcgcctgg tgggcgacgt gatggggaag taccaccccc
     2641 acggcgatgc cgcgatctac gacaccatcg tcggcatggc acaggatttc aagtcgcgct
     2701 atccgctggt ggccggccac ggcaacttcg gctccatcta cggcgacagc gcggcggcga
     2761 tgcgctatac cgaggtgcgc atggcgccgc tcgcccagga gatggtcgtc gacatcggcc
     2821 gcgacaccgt cgacatggtg ccgaactacg acaacaagca gctcgagccc gcggtgttgc
     2881 cctcgcgcat ccccaacctc ctgatcaacg ggagcatggg gatcgcggtt gtctacacca
     2941 cgaacattcc gccgcacaac ctgcgcgagg tgtgtgacgc ggcgctcctc tacatcgaca
     3001 accccaatgc caccgtcgac gagctgatga aggtgctgcc cgggcc
//
LOCUS       DAOSCV010000549         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20728_length_3045_cov_3.132441, whole genome shotgun sequence.
ACCESSION   DAOSCV010000549 DAOSCV010000000
VERSION     DAOSCV010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20728_length_3045_cov_3.132441"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..372
                     /gene="atpC"
                     /locus_tag="PLY56_13040"
     CDS             103..372
                     /gene="atpC"
                     /locus_tag="PLY56_13040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOJ22450.1"
                     /translation="MPERSFTLDIVTPEQLVLSEDVVSLTVPGVEGYLGILAGHAPLM
                     AELRSGVIEARRSDRTRVAMQIGGGFMEVSHNRAIVLADSAEPIA"
     gene            573..1997
                     /locus_tag="PLY56_13045"
     CDS             573..1997
                     /locus_tag="PLY56_13045"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOJ22451.1"
                     /translation="MSELVLPPVSLAAPARRKPVAGEWWMGVYLGESKLGHVQITVAP
                     ARGLRGGGYRIRTTTWTRALALGYVQEQRVEVDEHLSRDLRPVTLKVHMGSAGKQVEV
                     KARFHETVVEVERRAQDDVVRKSLPIPEGAHLVADVESALLGHAVAPGESLHYFFLNT
                     VTLSLEEATARVVRRERVRHDGEEYDTVVVQTTSPTVDATSWLLDDGQPVKMVMHPGI
                     TLVRESREKALSGFDDRASEPIRDLAEQLAVQMEGSITAPESTRFLRLRLRGLPERRF
                     VLSDARQVARIVEEGDALTVEYTVDATAAHPAPLTPAERAALLAPSAYLQVTHPDIQA
                     KATEIVAGATSDDARIQALWAWVHGNLRIQGDIGLPRTALDVLREPVGMCRDYATLYA
                     ALARAVGIPTRVCAGIVYFRDRFYYHAWAESYAEGCWVPVDPTVAPGFVDATHIKFTQ
                     GDAATMYGAVKSIGRLHATILEQR"
     gene            2275..2676
                     /locus_tag="PLY56_13050"
     CDS             2275..2676
                     /locus_tag="PLY56_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOJ22452.1"
                     /translation="MPTPISASPAADWLPVAVFFFIAVVVSVVMIVIPRMIGPGHPTP
                     QKTRPYECGERVVGMSWPQMHIGYYLYALVFIIFDVEVVFLWPWVRVVGGRLVQAHGL
                     GLFAFLDMLVFVTILVVGLVYAWRKGVLRWE"
     gene            2757..>3045
                     /locus_tag="PLY56_13055"
     CDS             2757..>3045
                     /locus_tag="PLY56_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22453.1"
                     /translation="MPAGEAASLDPAKLPVGVLERWPDGGVLTTDAESLRRYLERALD
                     AGEVALNWSRKSSLWYLLFGLACCAIEMMATGAARYDFDRFGMMFRASPRQA"
BASE COUNT          475 a          967 c         1047 g          556 t
ORIGIN      
        1 gtcggcggca ttgaggatgt tctggcccag gccgacaaga tgcgcgccgc cgcgtaaggt
       61 gcagagcgcc tttcgaggcg ctctggcctg ggaatctgga tcatgcctga acgaagcttc
      121 acgctcgata tagtgacccc cgagcagctg gtgctgagcg aggatgtcgt ttcgcttacc
      181 gttccggggg tggaaggcta cctggggata ttggcagggc atgcgccgct gatggccgag
      241 ttgcgcagcg gcgtcattga ggcgcgccgc agcgaccgaa cccgggtcgc catgcagatc
      301 ggcggcggtt tcatggaggt atcgcataac cgcgcgatcg tcctggccga ctccgccgag
      361 ccgattgcct agcctcgttg ggaagcacgc tctcgcccgg aagcggcgag cggggattcc
      421 tggccatggc cgcgtggcaa caggcaagca ggggcagaac gcgccaggct gctggctcaa
      481 gcgggtgggg atggcttcac ggcccgctcc acctgccttt gcccgcgctc tcccgcacca
      541 ccctcgccgt cgctctggga gtgctgctgc tcatgagtga actcgtgctt ccgcctgtct
      601 ccctggctgc tcccgcaaga cggaagccgg tggcgggcga gtggtggatg ggagtctacc
      661 tgggcgagag caagctcggt catgtgcaga tcaccgtggc gcctgcccga ggcttgaggg
      721 gcgggggcta ccgtatccgc accaccacct ggacgcgcgc tctggcgctg ggctacgtgc
      781 aggaacagcg cgtcgaggtg gatgagcacc tatcgcgcga cctgcgcccg gtcactctga
      841 aggtgcacat gggctcggct ggcaagcagg tggaggtgaa agcccgcttc cacgagaccg
      901 tggtcgaggt cgagcgccgg gcacaggacg acgtggtccg aaagtcgctg ccgatcccag
      961 agggcgccca tctagttgcc gacgtggaga gcgcgctcct gggccacgcg gtggcgcctg
     1021 gagagagcct tcactacttc ttcctcaaca cggtcaccct ctcgctggaa gaagcgactg
     1081 cgcgcgttgt gcgccgggag cgagttcgcc acgacggcga ggagtacgac acggttgtgg
     1141 tgcagaccac ctcaccgacg gtggacgcta cctcttggct gctagacgac ggccagccgg
     1201 tcaagatggt gatgcatccc gggatcaccc tggtgcgcga gagccgcgag aaagccctca
     1261 gcggcttcga tgaccgcgcc agcgagccaa tccgggatct ggcggaacag ctcgccgtac
     1321 agatggaagg atcgatcacg gcgcctgaat ccacccggtt tctgcgcctg cgcctgcgcg
     1381 gcctccccga gcggcggttt gtcctctccg atgcccggca ggtggcgcgc attgtggagg
     1441 agggcgatgc tctcacggtg gagtacactg tggatgccac cgcggcgcac ccggcgccgc
     1501 ttacccccgc cgagcgtgcc gcgctgctgg cgcccagcgc ctaccttcaa gtgacccacc
     1561 cggatattca ggcgaaagcc acggagattg tggcaggagc cacctcggac gacgcgcgga
     1621 tccaggcgct gtgggcgtgg gtgcacggga atctgcgcat ccagggcgac atcggtctcc
     1681 cacgcacggc gctcgatgtg ttgcgcgagc cggtgggaat gtgccgcgac tatgccaccc
     1741 tctatgccgc gctcgcgcgc gccgtcggga tccccacgcg cgtgtgtgcc ggcattgtct
     1801 atttccgcga ccgcttctat taccacgcct gggccgaaag ctatgcggag ggctgttggg
     1861 tgccggtcga tcccacggtt gcccccggat tcgtcgatgc cacgcatatc aagttcaccc
     1921 agggcgacgc cgccacaatg tacggcgccg tgaagagcat cggccggctc cacgccacga
     1981 tcctggagca acggtgaggc gataccgcgc ctgagcggcc ccaacgcgga tcggtatcgc
     2041 cgggtgaacc gcaacagttc ccgcgtggcg ggcggctacc gcgggcccca gcgctcgcca
     2101 tccgcggtcg caccctgcac cggtggcatt gcggcccgtg cctgccgggg gctgtgtatc
     2161 agtgtattcc cttattcgac tcaagggatg cccggattcc acctgcgtga gtgccagcag
     2221 ccttgacgat cctcggccgc aggagtataa tagcccccag agggagattg ttgaatgccg
     2281 acgcccatct cggccagccc ggcagccgac tggttgccgg tggcggtatt cttcttcatc
     2341 gctgtggtcg tctccgtggt gatgatcgtc atcccccgga tgattggtcc ggggcatccc
     2401 acaccccaga agactcgtcc atatgagtgt ggcgagcggg tggtaggaat gtcctggccg
     2461 caaatgcaca tcggctacta cctctatgcc ctcgtcttca tcatcttcga tgtggaagta
     2521 gtcttcctct ggccgtgggt gcgcgtggtg ggcggccggt tggtgcaggc gcacggtctc
     2581 ggtctctttg cctttctcga tatgctcgtc tttgtgacca ttctggtggt cggtctggtg
     2641 tacgcatgga ggaaaggggt cctgcggtgg gagtagatcg ccagtcgccg ggagggccgg
     2701 cagagaagcc aaccgctggc caggggcgag tggtggacgc cactgcgctt ccggtattgc
     2761 ccgctggcga agccgcttcc ctcgatcctg caaagctccc cgtgggtgtg ttggagcgct
     2821 ggccggacgg cggcgtcctg acgacggacg cggagagcct ccgccggtat ctggagcgcg
     2881 cgctcgatgc gggggaagtc gcgctcaact ggagccggaa gtcgtcgctg tggtatctcc
     2941 tcttcgggtt ggcatgctgc gccattgaga tgatggcgac gggcgccgcg cgctatgatt
     3001 tcgaccgctt cgggatgatg ttccgcgcca gtccccggca ggccg
//
LOCUS       DAOSCV010000550         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20761_length_3041_cov_4.068319, whole genome shotgun sequence.
ACCESSION   DAOSCV010000550 DAOSCV010000000
VERSION     DAOSCV010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20761_length_3041_cov_4.068319"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..2268)
                     /locus_tag="PLY56_13060"
     CDS             complement(145..2268)
                     /locus_tag="PLY56_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOJ22454.1"
                     /translation="MTLWIEDPKRYWERISFVRRAFSVLSALSLLVTLALAVSFYREN
                     RAAQDRVLERASEQTRHTGNRLDGVCRRVMHVADTLAADLSRARISEDRLLDRLRVLQ
                     RSHAVVYSAGVRYAPFKRSSRRHVFSPYIVRGTGGEEPSILWREEGTDEENKWFSRAL
                     AEGPLWGEPRFAPEAKRLIITYYAPFTREDKETGAREPIGVVEISCSANDLAALLFSF
                     DLGAPGYAFLTTRTGRFLCAPASDWIRDGKSVASLAAERNDAALASFAASGLQGKPFC
                     FRDIDRSTGRPAWYIGEPIPSSGWVLGSVTDAGPLWRSEATTARRLLQMEIAALLFLL
                     TFLGARLAETYAARRRALLWTGSAVASLAIAAAIAHAWCLAYTLPPEPDARLTPVFTR
                     AGHGRLLRERLQQAREGGEEPAAVIPTGLILRSLAFSGASDVEMAGEIWQQYDRIRHE
                     GVPRGVRLLGTLGYTLSDERREEAGNTEWVRWQFRARLRGGHDYRHYPFDHQQTVVGV
                     APPEGETPIILVPDVASYDRLHPAACPGIAADVQLTGREIERSFFAYLPDQPGEGAGA
                     QKGKLPALGFVVQTRHSVPAIVADTLVVLLAMIAALFLLLFVIARPARGSVALSAIFT
                     AASLLLALVAHMTLRHHHPVPQMTYAGYLCITLYAVVLVASAIAGFAGRRDGEDAEEA
                     RLLPSLLLGPGSLALLYIVTLAVLR"
     gene            complement(2544..>3041)
                     /locus_tag="PLY56_13065"
     CDS             complement(2544..>3041)
                     /locus_tag="PLY56_13065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22455.1"
                     /translation="KQMGLAIMQYVQDYDETYPIGGMLDSKGVQIHWRHLIYPYVKNE
                     GVFKCPSMGTYSYTAAHPALGAPALAGSYGCNYLVMRWNVASDMPSMPRPADCVAVAE
                     RRTGADWPAYPSNQVPDPSYVADGIVSDRHMEGANYIFCDGHAKWLRHGTDVTPLNLW
                     DPNKK"
BASE COUNT          540 a          970 c         1050 g          481 t
ORIGIN      
        1 atgaaaagaa gaagtctctc tgccaggcat ggcccgggtt cgtgggcact gcccaaaaac
       61 actctgccac cgggcgcttg tggcggcaac ggcgtgccac caggcaagag acttctcttg
      121 aagtattctg gtgaatactt cgggctatcg gagtacggcg agcgtgacga tgtaaagcag
      181 agccaagctg cccggcccga ggagcaggct ggggaggagg cgggcctctt cggcgtcctc
      241 gccgtcgcgg cgtccggcga acccggcgat ggcggaggcc acgaggacca cggcgtacag
      301 ggtgatgcag agatacccgg cataggtcat ttgtgggacc ggatggtggt gccgcaaggt
      361 catatgggcg acgagggcga gcaagagcga agccgccgtg aagatcgcgc tcagggcgac
      421 ggaaccacgc gccgggcgcg cgatcacgaa gaggagcagg aagagggctg cgatcatcgc
      481 gagcagcacc accagagtat cggcgacgat ggcgggcacg ctatgccggg tttgcaccac
      541 gaagccaagc gcggggagct tccccttctg cgcgcccgct ccctccccgg gctggtccgg
      601 gagataagca aagaagctgc gttcgatctc tcggccggtg agttggacat ccgccgcgat
      661 ccccgggcat gcggcggggt gcagccggtc gtaggaagcg acatcaggca ccaggatgat
      721 cggcgtctcc ccctccggcg gcgccacgcc cacgaccgtt tgctgatgat cgaagggata
      781 gtgccggtaa tcgtggccgc cgcgcagccg ggcgcggaac tgccagcgca cccactccgt
      841 gttccctgct tcctcgcgcc tctcgtcact gagcgtgtat cccagcgtgc cgagcaggcg
      901 cacgccgcgg ggaacgcctt catgccggat acggtcgtac tgctgccaga tctctccggc
      961 catctccacg tcgctcgcgc cagagaaggc gagtgaacgg aggatcaacc cggtggggat
     1021 cacggctgcc ggctcctcgc ccccttcacg tgcctgctgg agacgctcgc gaaggagccg
     1081 gccgtggccg gcacgtgtga agaccggcgt caggcgggca tccggctccg ggggcagggt
     1141 gtaggcgagg caccaggcgt gggcgatagc ggccgctatc gccagggacg ccacggcgct
     1201 tcccgtccag agaagcgctc gccgccgcgc agcgtaggtc tccgccagcc gcgcgccgag
     1261 gaaggtgagc aggaagagga gcgccgcgat ctccatctgg aggaggcgcc ttgccgtggt
     1321 tgcttccgaa cgccacagcg gcccggcatc ggtgacgctg cccaggaccc agccgctgct
     1381 cgggatgggt tcgccgatgt accaggccgg ccggccggtg ctccggtcga tgtcgcggaa
     1441 gcagaacggc ttgccctgaa gcccgctcgc ggcgaaggac gcaagcgccg cgtcgttccg
     1501 ctccgccgcg agcgaagcca cgctcttgcc atcgcgaatc caatcactcg caggcgcgca
     1561 gaggaaccgg cctgtgcgcg tggtgaggaa ggcgtagccg ggagctccca ggtcgaacga
     1621 gaagaggagc gccgccaggt cattcgccga gcaggagatc tccactacgc cgatcggctc
     1681 gcgcgctccg gtctctttat cctcccgggt gaagggtgcg tagtaggtga tgatgaggcg
     1741 cttcgcctcc ggggcgaagc gcggctcgcc ccagagcggt ccctcggcga gcgcccggga
     1801 aaaccacttg ttctcctcgt cagtcccctc ttcgcgccag aggatgcttg gctcctcgcc
     1861 gccggtgccg cggacgatgt acgggctaaa gacatgccgt cggctcgatc gcttgaaggg
     1921 tgcgtacctc acgcccgcgc tgtagacgac cgcgtgtgac cgctgaagca cgcgcaagcg
     1981 gtccagaaga cgatcctcgg agatacgcgc gcggctgaga tcggcggcca gcgtatcggc
     2041 gacgtgcatc acccgccggc acacgccatc gaggcgattg ccggtgtgac gtgtctgttc
     2101 ggatgctctt tccagcacgc gatcctgcgc cgcgcggttc tcccgataga aggaaacggc
     2161 tagagcaaga gtgaccaaga gagagagtgc gctcagcacg gagaaggcgc gccgcacaaa
     2221 agagatgcgc tcccagtagc gcttggggtc ctcgatccac aaggtcatct gcactcccac
     2281 ggcggcacgg cgagaggccg gtgccgtatc gccgggctta tgccctgcga cggtgcaaga
     2341 ttcgccgcgc gtggcggtga ttcccgcatc gtgatcaagg agaacagaaa cgtcctggga
     2401 gcgcgcacct agcatggaaa cgcccgccgg cctccgatgg cgtcaccaac cgggtgcctg
     2461 tttccgtgcg cctggagtga gcgcgtcgag gtgggaggtg ggaggccgca ggcactcccc
     2521 cccacacggc atgcgcttcc gcgtcacttc ttgttcggat cccacaggtt gaggggggtc
     2581 acatccgtgc cgtggcgcag ccatttggcg tggccgtcgc agaagatgta gttggcaccc
     2641 tccatgtgcc ggtcgcttac gatgccatcg gcgacatatg agggatccgg cacctgattg
     2701 gacggatagg cgggccagtc cgcgccggtg cggcgctctg cgacggcgac gcagtctgct
     2761 gggcggggca tgctgggcat atcgctcgcc acgttccagc gcatcaccag atagttgcag
     2821 ccgtaggagc cggccagcgc cggagcaccg agagccgggt gtgccgcggt gtagctatag
     2881 gtacccatgc tcgggcactt gaagacgcct tcgttcttga cgtaggggta gatcaggtgg
     2941 cgccagtgga tctgaacgcc cttcgaatca agcatcccgc caatcggata ggtctcgtca
     3001 taatcctgaa catactgcat gatcgcgagg cccatctgct t
//
LOCUS       DAOSCV010000551         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20804_length_3037_cov_3.302146, whole genome shotgun sequence.
ACCESSION   DAOSCV010000551 DAOSCV010000000
VERSION     DAOSCV010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20804_length_3037_cov_3.302146"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   9..481
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=9..45
                     /rpt_unit_seq="gtagcaccgggcctccgggcccggtgaggattgaaac"
     gene            1375..2745
                     /locus_tag="PLY56_13070"
     CDS             1375..2745
                     /locus_tag="PLY56_13070"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HOJ22456.1"
                     /translation="MAQLCGSRSQGALRVIAVVACVLFGPPAAGAPYYNYPGAIHIHT
                     VYSDGSGTFQEVAETAEATQLAYIITTDHNTLEPIRDGHQRYWGRVLVLVGTEISTDA
                     GHYLALDLPGSFEWGTRDAQQVIDRVNAAGGFGVLAHPVSERWPWTDWSVTSIAGLEI
                     INLASLMDADLRAVTQMNLPGRSLRRLAGLAQRYLTNPDAVMASFANNTVDRERTQWD
                     ALLREGRQYIGTAGVDAHARIPVAGTVYRVPTYREAFETVQTYAVALEPLRGDAESDR
                     REIYRAYRNGRLYMVYPRVAPAPEFRFVAEEAGRVATMGQPITLADRVRLTVEAPDHS
                     HPIIRLLRNGTEVASAEEARLEWDATQPGVYRVEVYAGVGAGRLFDIRRGLRLPRVEE
                     LLRSRRQELQPWIMSNPIYVRGTGTSAQGEDPVFPAIGTVRRASRIDPHYAALEAAGI
                     CCCMLP"
BASE COUNT          533 a          958 c         1018 g          528 t
ORIGIN      
        1 gcatccaggt agcaccgggc ctccgggccc ggtgaggatt gaaacaagtg cttacgcttc
       61 accttcgcaa cggaagccga tagtagcacc gggcctccgg gcccggtgag gattgaaacg
      121 tcatcggcgt agacgccctc cacctcccgc tgccagtagc accgggcctc cgggcccggt
      181 gaggattgaa acgaaccggc agcgttcgat ccggaatgcc tctgggaggt agcaccgggc
      241 ctccgggccc ggtgaggatt gaaacgcgcc ttcaggcgca gattactgcg cagttcgcgc
      301 gtagcaccgg gcctccgggc ccggtgagga ttgagactag gctatgctat aatacgggca
      361 gaatgatatt ggtggcaccg gggcttcggg cccggtgagg gttgagacga gcgccagtgc
      421 ctggagtgcg tccgcgacgc gcgagtggca ccggggcttc gggcccggtg agggttgaga
      481 cagtggagat gtagttcgtt gtgcctccgt tcgggcagat cccggacatt ctggattccc
      541 tcccggacgg ggagatagcg cactcggagc tccggtccac gatggcctac ttcctcggga
      601 agtactgcag acgcgccgga ctacgcatct acacatcgat tctatcgctc gtccatcacc
      661 gcgaggtgag gagtcccctt ggccaccggg cgtgctatgg gcacgcgtcc ggtagtttca
      721 ctcggcacta cgggccgatg gagccagcct aatgcgcttc gagcagccgt tcttcgtgac
      781 cccgcatgca gttcgggcgt ttcaaacgcg cgtctgtcct ggagccaccg cggcggacgc
      841 tattcgccag gtgcaggcag cattacaaga cccgatcgcg tgtcacccag gcagggacgg
      901 tgcggttgtg ttcctgtgcg agctcccgaa tgggaaaagg ttcggcgccg tggtcaaacc
      961 ggcgccagac gggctctcct ggcccgtcgt cacaaccacg gggcaggcat ggatgtggca
     1021 cgagttcaac cggtatcaga aggtgccagg caagcgccaa gttataccgg gccggacagc
     1081 ccggtgagga ttgaaaccac cgcgaggggt atcggcatct gaggagcctg atgctgcacc
     1141 gggtcgcagg cccggtgagg attgaaacgc atgcgcatgt cgcggtggaa tgtagacacg
     1201 gcctcccgct cctggcgtgg gggcaagccc tgggctccat ctagcgacac gccttggagc
     1261 cccgaaggca catggcacgt ttgactggta tcggtggcgg gtaagcccct cctgaactcg
     1321 ccatcgctta tgtccggtag cagtttggat cataagggta gaaggaggga ccccatggcg
     1381 cagctatgcg gctctcgctc ccagggggct ctaagggtga tcgccgtcgt ggcctgtgtc
     1441 ctgttcgggc cacccgcggc cggcgcaccc tactacaact atccaggcgc catccacatc
     1501 cacacggtct attccgatgg cagcggcacg tttcaggagg tagccgaaac ggccgaggcg
     1561 acccagcttg cctacatcat cacgaccgat cataatacgc tcgagccaat ccgagatggg
     1621 catcaacgct actggggccg ggtcctggtt cttgtaggca ccgagatcag cacggacgcg
     1681 ggacactacc tagccctcga tctgcccggc agcttcgaat ggggcacgcg cgacgcgcag
     1741 caggtcattg accgggtgaa tgccgccggc gggttcggtg tcctcgcaca tcccgtttcg
     1801 gagcgttggc cttggaccga ttggagtgtg accagcatcg ccggcttgga gatcatcaac
     1861 ctcgctagcc ttatggatgc ggatctgcgc gcggtgaccc agatgaacct gccggggcgg
     1921 agcctgagaa ggctggccgg gctggcacag cgctacctca cgaatccaga tgccgtcatg
     1981 gccagctttg ccaacaacac ggtggatcgc gagcgcacgc agtgggatgc tctcctccgc
     2041 gagggccggc agtacatcgg cacggcgggc gtggatgccc atgcgcgcat tcctgtcgcg
     2101 ggcaccgtct accgggtacc aacctaccga gaggcgttcg agacggtaca gacgtatgca
     2161 gtggccctgg agcctctgag gggcgacgcg gagagcgacc gccgggagat ctatcgtgcc
     2221 taccggaatg ggcgcctcta catggtctac ccccgtgtcg cgccagcacc ggagttccgc
     2281 ttcgtggccg aggaagcagg ccgggttgcg accatggggc aaccgatcac gctcgccgac
     2341 agggtgcgcc tcacggtgga agcgccggac cattcccatc ccatcatccg tctgttgcgc
     2401 aacggcaccg aggttgccag cgcggaggaa gcccgcctgg agtgggatgc gacgcagcca
     2461 ggggtctacc gggtggaggt ctacgcgggc gtgggcgccg gccggctctt tgatattcgc
     2521 cgcggcctgc gcttgcccag ggtggaagag ctcctccgct cgcgccgcca agagctgcaa
     2581 ccgtggatta tgagcaaccc gatctatgtg cgtggcacgg gcacctcggc acagggtgag
     2641 gacccggtgt tcccggccat cggcacagtg cgtagggcct cgcgcatcga cccgcactac
     2701 gcggcactgg aggcggcggg catctgctgc tgcatgctcc cataagacgg ttttcccggg
     2761 ggcaagagtt cctcttgcca cgtgggggcg ccactcccgc gctgagattc tgagagaagg
     2821 ccgctccggg cggctgtccg cgctcgcgtg gggatttcga actccgggat cgcggtctgg
     2881 cccgcttccc cggctccctc gcagccctcc ggccctgcat cgccatcgat accacagatg
     2941 aaggagaccg ccgcggatgg gttcctatgc taggattccg acataaggag agggccgccc
     3001 gcacgcgcgg agctcgaagc tccgcgctgc ttcccgg
//
LOCUS       DAOSCV010000552         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20818_length_3035_cov_3.502349, whole genome shotgun sequence.
ACCESSION   DAOSCV010000552 DAOSCV010000000
VERSION     DAOSCV010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20818_length_3035_cov_3.502349"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLY56_13075"
     CDS             <1..619
                     /locus_tag="PLY56_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22457.1"
                     /translation="VCVLWGVHHRRWQIAAGLGITLAVAVGAVACVDPLILSRYQEMM
                     AGDPALPPLTLTATTGAMLRLLLGAEKAWLQTAPLAVGACWTCLYYWRRRRSWDWMEH
                     TPLLLAASVIVTPYAWPPDYVLLLPAILQAATRVSGAGTRPIGLALLFYVPVVAACFT
                     VWFSGVAGVWHAWQPWAVLGACLIARKLATPARIPSTASNLGTLR"
     gene            616..>1735
                     /locus_tag="PLY56_13080"
     CDS             616..>1735
                     /locus_tag="PLY56_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22458.1"
                     /translation="MKEYRTVGAQRWCLAAMRGVPLAFLVFSALMAVTKLLLDLWYPY
                     GLDYGEGVVLDGVRATLEGRTLYPPLDETFYAVSIYMPLYVWVCAAVAWLTAPTFLVG
                     RIVSLALTLVCAGLVYALARREGASRWWSAMGAGLFLTSIPVVAWGAYMRVDMMGLAF
                     SLGGLLVLSSGASPLWAGLLFSLAILSKQSMLAALFGASLFLAARRRWRELGLLLLAT
                     IVPVAAVCAWQMVATEGWFLFHTVKANGVRYSVSWASRYLGEGLVPHTALAVLALAGW
                     WAAGCPGSPRRPSLLTGYLIGACGAALMVGKVGAVVNYFLDYVAVLSILAAIGISRVV
                     GEGELRSLYREAFVAAALLIHLGAVTRMPDIAEYVETLP"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1746..2087
                     /locus_tag="PLY56_13085"
     CDS             <1746..2087
                     /locus_tag="PLY56_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22459.1"
                     /translation="RVTSLLAESRGPVLSDDPSVVAGAGQRLVLEPFQATQMDLSGIW
                     SAGPLVARLRQGDFPLIVLRFDPWNPAHRLPDGTWASGRWSDAMAEAVRHAYRVDERW
                     KGFLVLRPRGE"
     gene            complement(2245..2511)
                     /locus_tag="PLY56_13090"
     CDS             complement(2245..2511)
                     /locus_tag="PLY56_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22460.1"
                     /translation="MWATPTVRMLPLTYTLRYKSGAGLFLGGGAGMYYCTVDEPAEDS
                     LHKWKGGVHGCVGYDMGALALELRYSNIFNTKMSGFSVSAGAVF"
BASE COUNT          434 a          950 c         1043 g          598 t
ORIGIN      
        1 ggtgtgcgtg ctctgggggg tccatcaccg gcggtggcag attgccgcgg ggttgggcat
       61 caccctcgcg gtcgcagtag gggctgttgc ttgcgtggat cccctcatcc tctctcgcta
      121 tcaggagatg atggcgggcg acccggcgct cccgccgctg acgctgaccg cgacgacagg
      181 agcgatgctg cgcttgctgc tcggcgcaga gaaagcctgg cttcagacgg cgccactggc
      241 tgtcggtgcc tgctggacgt gcctctacta ctggcggcgg cgccgctcgt gggattggat
      301 ggagcatacg ccactgctgc ttgccgcgtc tgtgatcgtg acgccctatg cgtggccgcc
      361 cgattacgtg ctgcttctcc ccgcgatcct ccaggccgcg acgagggtct ctggcgcggg
      421 gactaggcca attggtctcg ccctcctctt ctacgtgccg gtggtagcgg catgcttcac
      481 cgtctggttt agcggagtgg cgggagtgtg gcacgcctgg cagccatggg ctgtgctggg
      541 cgcctgcctg atcgcgagga agcttgcgac gccagcgcgc ataccttcca cggcgagcaa
      601 cctagggacc ctgagatgaa ggagtatcgt actgtgggcg cgcagaggtg gtgcctcgcc
      661 gcgatgcgag gcgttccgct ggcgtttctg gtgttctcgg cgctgatggc tgtgaccaag
      721 cttctgctcg acctgtggta cccctatggt ctcgactatg gcgagggcgt ggtactcgac
      781 ggtgtgcgcg ccacgctgga aggccgaacg ctctacccgc cgctcgatga gaccttctat
      841 gccgtttcca tctacatgcc gctttacgta tgggtgtgcg cggcggtggc ctggctcacg
      901 gctcccactt ttctggtggg ccggatcgtg tctctcgcgc tcaccctcgt gtgcgcgggg
      961 ctggtctatg ccctggcccg tcgggagggg gcatcgcgct ggtggagcgc aatgggcgcc
     1021 gggctcttcc tcaccagcat ccctgtggtg gcgtggggcg catacatgcg cgtggatatg
     1081 atggggctgg ccttcagcct cggcggtctg ctcgtcttga gctccggcgc gtcccctctc
     1141 tgggcggggc tgctcttctc gctggcgatc ctgagcaagc agtcgatgct cgcggcgctc
     1201 ttcggggcgt ctctctttct cgctgcccgg cgccgctggc gcgagctagg gctgctgctg
     1261 ctcgcgacca tagttccagt ggcggcggtg tgcgcgtggc agatggttgc aacagagggg
     1321 tggtttctct tccacacggt gaaggccaac ggcgtgcgtt acagcgtcag ctgggcttcg
     1381 cgctacctgg gagaagggct cgtaccgcac accgcgctcg cggtgctggc actcgcgggg
     1441 tggtgggccg cgggatgtcc tgggtcgccg cggcgcccgt cgctcctcac gggctatctg
     1501 atcggcgcat gcggcgccgc gctgatggta ggcaaggtag gcgcggtggt caactacttc
     1561 ctggactatg tcgccgtgct ctcgatcctg gcggccatcg ggataagccg ggtggtgggg
     1621 gaaggcgagc tgcgctcgct ctatcgcgag gcgttcgtgg cagcagcgct cctgatccac
     1681 ctgggagccg tgacgcgcat gcccgatata gccgagtatg tggagacact gccggnnnnn
     1741 nnnnncgcgt gacgagcctg ctcgcggagt cgcgcgggcc cgtgctctcg gatgatccca
     1801 gcgtggtggc cggcgctgga cagcggctgg tcctggagcc cttccaggcc actcaaatgg
     1861 acctgagcgg gatctggagt gcgggtccgc tggtggcccg cctgcgccag ggcgatttcc
     1921 ccctcattgt cctccggttc gatccctgga acccggcgca ccggttgcca gacggcacct
     1981 gggcgagtgg ccggtggagc gatgcaatgg cggaggcggt gcgccacgcc tatcgcgtgg
     2041 atgagcgctg gaaggggttc cttgtcctgc gcccccgtgg cgaataggac gcctggcgcg
     2101 cgggactcag tcccataata gccccgaaag agggaagcgg gcgggcgttt ctgggttgct
     2161 gttcgaaccg gcgccgggcc ctacggcccg gcgccatggg gctggcagca ctctatcacg
     2221 ccagaccagg tgctgctgtt caggttagaa gacagcgccc gccgatacgg aaaagccgct
     2281 catcttcgtg ttgaagatat tggaataccg caactcgagc gccagcgccc ccatgtcgta
     2341 accgacacag ccgtgaacgc cacccttcca tttgtgaagg gagtcctcgg cgggctcgtc
     2401 gacagtgcag tagtacatgc cggcaccgcc gcccaggaag agaccggcac cactcttgta
     2461 gcgcagcgtg taggtcagcg gcagcatgcg aacggtgggc gtcgcccaca ccgatgtctt
     2521 tgctcttcca gatcattccc acgccaaagc tgtgctccag ttggggatcg ctctggatgt
     2581 tgtagttggc gcccagctgc cagaagggcg agccaatcaa atcgaccgct acgtcgtcgg
     2641 tcgggaaata caggccgccc gtcacggtaa agctgtccct cggtccggcg agggcgacgc
     2701 tgccagcgag tacagtcaga accgatgcaa tcacgcagat ttatgatagc gacttcatcc
     2761 tctttccttt ctcttgctcg attgaacctt ctcccgcaag cgccccgcag gggttctctg
     2821 agccccgctg cgaagtacaa ctctgtttgt ccggcggagt gccggaacgc caggccgctt
     2881 cgcgagcggt tacgggcgag cgttacactc gcgaccggct cagtaacctg gcccagcggg
     2941 tctgcccgcc gcttcgcgag cggttacggg cgagcgttac acctcgagcg aaggactaag
     3001 gactccggcc gggcaatggc agggatcccg cgggt
//
LOCUS       DAOSCV010000553         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20910_length_3024_cov_3.196026, whole genome shotgun sequence.
ACCESSION   DAOSCV010000553 DAOSCV010000000
VERSION     DAOSCV010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20910_length_3024_cov_3.196026"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PLY56_13095"
     CDS             <1..1352
                     /locus_tag="PLY56_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22461.1"
                     /translation="EPVIEATHAFLAEPRQAISVRYSDNVGVKNVRLVLDGRDITRRA
                     KVGPTSAQFVPDRDWAPGLHKLDVEVADARGNRASRRLFFNRMPGVVKKEWLKSGGIA
                     IDGKPHFLLGMYGVRREDMPEMAAAGYDFVHNYAWDGTGSNESALEYLDEAQKHGLQA
                     FIGFNRARLQAWDEEFVAERVGALGRHPALLAWYLFDEPDLGHQYVPPDQLRALYQLI
                     KALDPLHPVIVTVAQTHLVPAYHHSYDVYWSMDYGNTAHVASNYDRHSELIGPDTPQM
                     SIVHCYDQKQKANLVPEKFQPGPAMLRANAFMAIAHGSSSLAWWWWGQGGSSFFTVAH
                     VPEAWAALKETVRQIRHLRPVLEAQVPARRWVEKPAEGVEIHLWERKLPDRSVIIAVN
                     RDEKACELTLASPLLANATQAKVLFEDRAVLVREGRLTDRFEPLGVHVYEVPTGAGR"
     gene            1661..>3024
                     /locus_tag="PLY56_13100"
     CDS             1661..>3024
                     /locus_tag="PLY56_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ22462.1"
                     /translation="MRKAVAILTLALMASASSLFAQERVKNGGLDFEAKLDGWKPGHA
                     SFRFADDDGCEAPGCIRFIGPAPAKAAGPSQVVTVKPNTDYVLAAAIKGDGKLAPSVR
                     LRGADGKDVVIAGGTRVTVWTPVRVRFNTGPHEKLTLVCVASDEAAPEGSRAAFDDIA
                     LLLPSEVPAGQSVQGGGLLKPPGENIALGCKVTFESRPNYGLCTDDEDIIQLTDGKYS
                     AGYFWVQKSTVGWNNVSPVTITIDLGKVQPIAGLSYNTAAGVAGVGWPSSILVAVSDD
                     GETWRFVGDLVTLSTKNGLPPADRYAVYRYATAELQTRGRYVRIATAPQGGFTFCDEI
                     EVYRGPESLLAKAPAGEIVTDMKTLTQRIATRSGVIFRLTADLAAAREALKASLLPDA
                     RKRALNERLDAVERAIPQLPDFDPAQFRAIFPLNELHTQILSVRGAVLSTRGLPRVFA
                     WKKNR"
BASE COUNT          529 a          990 c          999 g          506 t
ORIGIN      
        1 ccgagccggt catcgaggcg acccacgctt tccttgccga gccccggcag gcaatcagcg
       61 tgcgctactc cgacaatgtg ggtgtgaaga acgtacgcct tgtgctcgat gggcgcgaca
      121 tcacccggag ggccaaggtg gggccaacat cagcccagtt cgtccccgac cgagattggg
      181 cgccgggact gcacaagctc gatgtggaag tggccgatgc gcgtggcaac cgggcatcac
      241 gccggctgtt cttcaaccgg atgccgggcg tggtaaaaaa ggagtggctg aagagcggcg
      301 ggatcgccat cgacggcaag ccacacttcc tcctcggcat gtatggtgtg cgccgcgagg
      361 atatgccgga gatggccgcg gcaggctacg acttcgtcca caactacgcc tgggatggca
      421 ccgggagcaa cgagagtgcc ctggagtatc tggatgaggc gcagaagcat ggcctgcagg
      481 cgttcatcgg cttcaaccgc gcccggttgc aggcgtggga cgaggagttc gtcgccgagc
      541 gcgtcggcgc cctcggacgg cacccggcgc tccttgcctg gtacctcttt gacgagccgg
      601 atctcggcca ccagtatgtg cctcccgacc agctccgggc actctatcag ttgatcaagg
      661 cgctcgatcc gctccatccg gtgatcgtca ccgtggccca gacccacctg gtgcccgcgt
      721 atcaccatag ctacgatgtc tactggagta tggactacgg caacacggcg cacgtggcgt
      781 ccaactacga caggcacagc gagctcatcg gcccggacac gccgcagatg tcgattgtcc
      841 actgctacga ccagaagcag aaggctaacc tggttcctga gaagttccag ccgggcccgg
      901 cgatgctgcg cgccaacgcc ttcatggcga ttgcccacgg gagcagtagc ctggcctggt
      961 ggtggtgggg ccagggagga agctcctttt tcacggttgc ccacgtgccc gaggcatggg
     1021 ccgctctgaa ggagaccgtg cgccagatcc ggcaccttcg gcccgtgctg gaagcgcagg
     1081 tgcccgcgcg gagatgggtg gagaagcccg ccgagggcgt ggagatccac ctctgggagc
     1141 ggaagctgcc ggaccggagc gtgatcatcg ccgtcaatcg cgacgagaag gcgtgtgaac
     1201 tgacgctcgc ctctcccctg ctcgccaacg ccacgcaagc gaaggtgctt ttcgaggatc
     1261 gcgcggtgct cgtgcgggag ggccggctca ccgatcgctt cgagcccctg ggcgtgcatg
     1321 tctacgaggt gccaacgggc gccggccgct aaggcggcgc tcgcatcctg gcccccagtg
     1381 caccgcaacg ccccttacag gagagacgcg ccgcgtgcag aaaaagttat tacaacggta
     1441 ccggtgccgg ctccgaccag atccggaacc tgcacgggca ccgcttcctc ggcagctgct
     1501 tccgcgcccg tccggtgttg atggaagcac gcgttgggcc tgcccacacc gtcggcccgg
     1561 ggttgcggga ccgtggaacg ccctgccaga ggcatgcggc gttcttgccg gatggccctt
     1621 ggggtccctc cggccggccg atatggatag gaagtagctt atgagaaaag cagtggcaat
     1681 cctcacgctg gcgctgatgg cgtcggcatc ctcgctattc gcccaagagc gggtgaagaa
     1741 tggtggcctg gattttgagg ccaagctcga cgggtggaag cctggtcacg cgagcttccg
     1801 attcgcggac gatgatggct gcgaggctcc agggtgtatc cgtttcatcg gacccgcgcc
     1861 cgcgaaggca gctggcccct cgcaggtcgt cacggtaaag ccaaacaccg actacgtcct
     1921 tgccgccgcg atcaagggcg atggcaagct cgcgccttcc gtgcgcctgc gcggcgctga
     1981 tggcaaggat gtcgtcatcg cgggcggaac gcgcgtgacg gtgtggactc cagtgcgcgt
     2041 ccgcttcaac accggccctc atgagaagct gacgctcgtg tgcgtcgcgt ctgatgaggc
     2101 ggcacccgag ggctcccgcg ccgctttcga cgacatcgcg ctgcttctcc cctccgaagt
     2161 gcccgctggc caatctgtgc agggcggcgg cctgctgaag cctcctggtg agaatatcgc
     2221 cctggggtgc aaggtgacct tcgagagccg gcccaactac ggtctttgca ccgatgacga
     2281 agatatcatc cagctcaccg atgggaaata cagcgccggc tacttctggg tgcagaaaag
     2341 caccgtcggt tggaacaacg tctcgcccgt gacgatcacc atcgacctgg gcaaggtgca
     2401 gccgatcgcg ggcctctcct acaacaccgc ggccggcgtg gccggagtgg gttggcccag
     2461 ctctattctc gtcgcggtga gcgacgacgg cgagacatgg cggttcgtcg gcgatctcgt
     2521 caccctgtcg acgaagaatg gcctgccccc ggccgaccgc tacgcggttt accgctatgc
     2581 caccgcggaa ctgcagacgc gtggccgcta cgtgcgcatc gccaccgcgc cccagggtgg
     2641 gttcaccttc tgcgacgaga tcgaggtcta ccgcggtccc gagtcactcc tggcaaaggc
     2701 gccagcgggc gagatcgtga cggatatgaa gacgctgacc cagcgcatcg cgacacgctc
     2761 gggggtgatc ttccggctga ccgccgacct ggcagccgcc cgggaggcgc tgaaagcgtc
     2821 gcttctgccg gatgctcgca agcgcgccct gaacgagcgc ctggatgccg tggaacgcgc
     2881 catcccgcag cttcccgact tcgacccggc gcagttccga gcgatcttcc ccctcaacga
     2941 gctgcatacg cagatcctct cggtgcgcgg ggccgtgctg agcacgcgcg ggctgccacg
     3001 cgtcttcgcc tggaagaaga accg
//
LOCUS       DAOSCV010000554         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20962_length_3017_cov_4.391290, whole genome shotgun sequence.
ACCESSION   DAOSCV010000554 DAOSCV010000000
VERSION     DAOSCV010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20962_length_3017_cov_4.391290"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            766..2856
                     /locus_tag="PLY56_13105"
     CDS             766..2856
                     /locus_tag="PLY56_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOJ22463.1"
                     /translation="MSFSKRVSLRWLALQAMLWMSLMAVAGAAPAPRPPDVPGDHWAA
                     PAVLALLRPETNLVELFGDGSFQGGETLRRYDFVKNLDNLVRHLGRHGVRLGPGGPLP
                     DCPYTDVPEGHYSRDAIRRLLWMIPGSKNHFGGRQPMTRLDMAVWLHALLKDVPKAAS
                     VKPAPRPTDVDAHLAPMVCWALAAGEEGLISAYPDGRFHPDRALTRYEMAYTMYQVLR
                     WLTTAAPSPVEVVQQPGTQRDRAAPELRVFEPEVEGARDFVVVVRKKQLRVSGIAADD
                     TGIQQVTVNDHEAKLEFGDEADLVKADLKGPVAMWFHAQIPFPEANADGKSKLVIQVT
                     DKAKKRVTKTYTIGSPKPDAPQVVHASTQPEGTTRVGRKWALLVGVNRYDDVENIPTL
                     RYSVGDVTAVYKVLVDPQRGGFGKDTVYLLTDDTPDKPTNINIIKYLNRIARRAEPED
                     MVLVYFSGHGYEEAGRGYILPCNTDVEALAASAVDNTVLIDTLDRMKAQRIVTIIDAC
                     HSGAMRRTGGGAPALSGQYYSAYGSGKGRVVLASASAGQKAWEDARLKHGVFTEYVKQ
                     GFYGEADRNRDGAVTFMELAKYVYDNVKKWAEANNKEQEPMIQAENFAASDIILALNP
                     EALPTGPLAEKKRKVYAALEPEDADYACLLLEKSVPTDQEKRILKYLDEMIAGRITAQ
                     RYLDFVRMMNPGGR"
BASE COUNT          551 a          935 c         1006 g          525 t
ORIGIN      
        1 gaggcgctgg aagcaaccgg gaccaccgtg accatctccg aggccgatgc gcagcgcatt
       61 ctcgatgccg acaagacttc tgagttcctt gcccgttgcg ccgtgatcct cgtcgtgaag
      121 ccggaggggt agactcctgg gatttcggag ataaggaatg cgatccgggg cacagagatt
      181 cccggctcca atgcccagac ctctccggtc tcttctacca tagccctgcc ccgaagggac
      241 cgcacaactg agcccagggt atccgcacct cgggctgtgc tgctaccacg catttgtcga
      301 accctctcag ggttccaggt gttttctgga cgctagacct agggtcagga tgccctgggc
      361 tatactgtgg gaccacttcg cggtcttgcg agagcgtctc gcctgggcca tgccccgatc
      421 ccggatggac tgcgcagccc caggtgcccg catctctcca tcggtgttcg ggcgttcatc
      481 tgtggtaccg accccgcctc gggtgtccgg atacgagacg gtgtctccct cactcgttct
      541 cccaaccagc gtcttgccgc ctttgcgatg ccagacgccc tataggaatt gctccgaccg
      601 ccgataatca tagtgggaga tctgcacggg cacggcatgc gttcgaccct acggagaggc
      661 gagggcagct ctccggggcc ccgttcgccg catgcctgcc ctgggcgcgc atcgggccat
      721 gcctgtcgtc gccgagccac acgtgattct gcacgaggga gagagatgag cttcagcaaa
      781 cgagtgtccc tgcgctggtt ggcgctccag gccatgctct ggatgagcct gatggcagtg
      841 gccggcgccg caccggcgcc gcgtcccccg gacgtgcccg gggaccattg ggcggccccg
      901 gccgttcttg ccctgctccg tccggagacg aacctggtcg agctgttcgg cgatggctcg
      961 ttccagggtg gcgaaacgct ccgccgctac gacttcgtga aaaacctcga caacctggtg
     1021 cgccacctgg gcaggcacgg ggtgcgcctc gggccgggcg gcccgttgcc tgattgccca
     1081 tacacggatg ttccggaagg ccactactcg cgagacgcga tccggcgcct cctctggatg
     1141 attccgggga gcaagaacca ctttggcgga cggcagccga tgacgcggct cgatatggcg
     1201 gtgtggctgc acgcgcttct gaaggatgtg ccgaaagcgg cctccgtcaa gcccgccccc
     1261 aggccgaccg acgtggacgc gcatctggca ccgatggtct gctgggcgct ggccgcgggc
     1321 gaggaaggcc ttatctccgc ctatccggat ggccggttcc atcccgaccg cgcgctgaca
     1381 cgctacgaga tggcctatac gatgtaccag gtgttgcgct ggctgaccac ggcggcgccg
     1441 agccccgtgg aagtggtgca gcagcctggc acccagaggg atcgtgccgc tcccgagctc
     1501 cgcgttttcg agccggaggt ggagggcgcg cgcgatttcg tggtcgtcgt gcgcaagaag
     1561 cagctgcgag tgtcgggtat cgcggcggat gacaccggaa tccagcaggt gacggtcaac
     1621 gatcacgaag cgaagctgga gtttggtgac gaggcggacc tggtcaaggc ggatctcaag
     1681 ggtccggtgg cgatgtggtt ccacgcgcag atccccttcc cggaggccaa cgctgatggg
     1741 aaatccaagc tggtgatcca ggtcaccgat aaggcgaaga agcgcgtgac gaagacgtac
     1801 accatcggct cgccaaagcc ggacgcgccg caggtggtgc acgcctccac acagcccgag
     1861 ggtaccacgc gcgtagggcg caaatgggcg ctcctggtag gcgtcaatcg ttacgacgat
     1921 gtggagaaca tccccactct gcgctattcc gtgggagacg tgacggctgt ctacaaggtg
     1981 ctcgtcgatc cccagcgagg cgggtttggc aaggacaccg tctatctgct cacggacgac
     2041 acgccagata agccaacgaa tatcaatatc atcaagtacc tgaaccgcat tgcccgccgc
     2101 gccgagccgg aggatatggt gctggtctac ttctcgggcc atggctacga ggaagcgggc
     2161 cggggctata tcctgccatg caacaccgat gttgaggcgc tggcggccag cgcggtggac
     2221 aacacggtgc tcatcgatac gctcgaccgg atgaaggccc agcggatcgt gacgatcatt
     2281 gatgcgtgcc acagcggcgc catgcggcgc acgggtgggg gcgcgccggc cctcagcggg
     2341 cagtactaca gtgcctatgg ctctggcaag ggccgcgtgg tgcttgcctc tgcttccgcc
     2401 ggccagaaag catgggaaga cgcgcgcctg aagcacggcg ttttcaccga gtacgtcaag
     2461 cagggtttct atggtgaggc cgaccggaat cgcgacggcg ccgtcacctt catggagctc
     2521 gcgaagtacg tttacgacaa cgtgaagaag tgggcggaag cgaacaacaa ggagcaggag
     2581 ccgatgatcc aggcggagaa ctttgcggct tcggatatca tcctggccct gaacccggag
     2641 gcgctgccca ccggacctct ggcggagaag aagcggaagg tctatgccgc cctcgagcct
     2701 gaggatgcgg attatgcctg cttgctcctg gagaagtcgg tcccgaccga ccaggagaag
     2761 cggattctga agtacctgga tgagatgatc gccgggcgga tcacggccca gcgctatctc
     2821 gattttgtgc gcatgatgaa cccgggtggc cggtaggccc gcacgccggc ttagagacgt
     2881 tgcggtatac ccgcgtgcgc agcaggccag gagagatgag tatggaggca tccggcagcc
     2941 gccgtgaaag gcgcgaacga atggggctgg aggaggcagg cggcgatagg tcgccgtccc
     3001 actctgctcg tgcgccg
//
LOCUS       DAOSCV010000555         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_20992_length_3014_cov_3.153430, whole genome shotgun sequence.
ACCESSION   DAOSCV010000555 DAOSCV010000000
VERSION     DAOSCV010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20992_length_3014_cov_3.153430"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLY56_13110"
     CDS             complement(<1..1120)
                     /locus_tag="PLY56_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22464.1"
                     /translation="MFRAATLLYTVPLLLLAACRMVSAAPAPVPVANGGFEEGLGQWA
                     ALRPEGYGHGEFGITTAEAHTGKNAIRITTVPEGEKVLIGLTHGRMIALPDDSRTFRL
                     SVWLKANKAPKAIELRIASAGRDGRALTPWQEKGWRFIRPPVDPHVGKWHQAVAEFAA
                     QQEWGGLYLTVWINGAGADVLIDDISLEAVDPTDWMVASVGERLPDPNPGTALWWEGP
                     LRKVFPNEEPPKARGNGIALCAAGDEYEAVQLCVRPARAVEEARVSFTDLAGPRKIPA
                     SALNARFVGLIDVKEPKACRSYTGLTPDPLLPDETATLPAGQTTALWITLKVPRGTPA
                     GDYRGSVTLAGKGLKASVPLSVRVYGFDLPEHPRLRTIA"
     gene            complement(1242..1697)
                     /locus_tag="PLY56_13115"
     CDS             complement(1242..1697)
                     /locus_tag="PLY56_13115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOJ22465.1"
                     /translation="MADHAVSIGATLALETGPEPPRVVRQLMEEINSPGLRVNFDPAN
                     LLLWPPKIAERHNKPFDYEWAMSEFNPVEGLRTLIPYVVHAHAKDSVLHPDGSRAEVP
                     LGTGMTNWPELHKIFKANGYQGYYAIERECGENAMEDVRKAVQFLRSLD"
     gene            complement(1616..2077)
                     /locus_tag="PLY56_13120"
     CDS             complement(1616..2077)
                     /locus_tag="PLY56_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22466.1"
                     /translation="MKIAIIPTTGGSDPFRGMEIARELGVDGVHISAYGGGLALQDKT
                     SAERKEILKKIHSYGLEVSALIGWGGQVDLGEEKDLEKNIAWGKKLNEVAVDMANGLW
                     MAHCGIAPEDPSDPRFKRFRDSSVRSLTTPSASVPPSRLKPVRSPRAWCAS"
     gene            complement(2218..>3014)
                     /locus_tag="PLY56_13125"
     CDS             complement(2218..>3014)
                     /locus_tag="PLY56_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ22467.1"
                     /translation="EAGAELVDLECVSFTFPRSRFEELLHSGGRDPEPFLSAGGAHYF
                     LGGIRIDERGRATVPGLYAAGEAAGGLFGAGRLGGSALADTLVFGAIAGENAAAFATS
                     TPRPRWDATRTDQERARLEALLSGSVPAAEVTAGLRSLMWRKCGLTKAARSLEEAREE
                     VAELESRRADLGASSVAGLREALECGFMLTLARLIITASLRREESRGAFWRLDAPEPD
                     NARWVRNIVLRREQGEDCLEVHTPVMARLKEPSQPRIGPGCFDYLP"
BASE COUNT          490 a         1041 c          987 g          496 t
ORIGIN      
        1 gagcgatcgt cctcaagcgg ggatgctccg ggaggtcgaa gccatagacg cgaaccgaga
       61 gcgggacgga tgctttgagg cccttgccag cgagcgtcac acttccccgg tagtcgcctg
      121 ccggtgtgcc ccggggcacc ttcagggtaa tccagagcgc ggtcgtctgc ccggcgggca
      181 gcgtggcggt ctcgtccggg agaagcgggt cgggcgtgag cccggtgtaa gagcggcagg
      241 ctttcggctc cttcacatca atcaggccga cgaagcgcgc gtttagcgcc gaggcgggaa
      301 tcttccttgg gccggccaga tcggtgaacg aaacgcgagc ttcctcgacc gcccgtgcgg
      361 ggcgcacgca gagctgcacc gcctcatact cgtcgccggc ggcgcaaagc gcgatcccgt
      421 tcccccgtgc cttcggcggc tcctcgttgg ggaagacttt gcggagcggg ccttcccacc
      481 agagcgcggt tcccggattc gggtcgggaa gccgctcgcc caccgaggcg accatccaat
      541 cggtgggatc gacagcctcc aggctgatgt cgtcgatcag gacgtcggcg ccggcgccat
      601 tgatccagac ggtgaggtag aggccgcccc actcctgctg agcggcaaac tcggcgacgg
      661 cctggtgcca cttgcccaca tggggatcga caggcgggcg gatgaagcgc cagcccttct
      721 cctgccacgg cgtgagcgcc cgtccatccc ggccggccga ggcaatccgt aactcgatcg
      781 ctttcggcgc cttgttcgcc ttgagccaaa ccgatagccg gaaagtgcgc gagtcatccg
      841 ggagcgctat catccggccg tgggtgagtc cgataaggac cttctccccc tcgggcaccg
      901 tggtgattcg gatggcattc ttgcccgtgt gcgcctccgc ggtagtgatc ccgaactcgc
      961 cgtggccata gccctccgga cggagggctg cccactgccc caggccctcc tcgaagccgc
     1021 cattggctac cggcacgggc gccggcgccg cggacaccat ccgacacgcg gccaggagca
     1081 acaggggaac cgtgtagaga agagttgccg ctcgaaacat gaccacctcc aagacatcca
     1141 tggtgggggc tgccacggtg tcgcgccacg ccgggcgcct ggctgatcac ccaaacgggc
     1201 cagccaggcg ccatctacca tggcctctcc ggcaggccta ctcaatccag actgcggagg
     1261 aactgaacgg ccttgcgcac gtcctccatg gcgttctcgc cgcactcgcg ctcgatagcg
     1321 tagtagcctt gatagccgtt cgccttgaag atcttgtgca gctccggcca attggtcatg
     1381 ccggtgccca gaggcacctc ggccctgcta ccatccgggt ggaggaccga gtctttggcg
     1441 tgggcatgga ccacatacgg gatcagggtg cgcaggccct cgacgggatt gaactcgctc
     1501 atggcccact catagtcgaa tggcttgttg tgccgctcgg cgatcttcgg cggccagagg
     1561 agcaggttgg ctgggtcgaa gttgacgcgc agcccggggc tgttgatctc ctccattagc
     1621 tggcgcacca cgcgcggggg ctccggaccg gtttcaagcg cgagggtggc accgatgctg
     1681 acggcgtggt cagcgatctc accgaggagt cgcggaagcg cttgaagcgc gggtcgctcg
     1741 ggtcctcggg ggcgatgccg cagtgggcca tccagaggcc gttggccata tccaccgcca
     1801 cctcattgag cttcttgccc caggcgatgt tcttctcgag atccttctcc tcgcccagat
     1861 ccacctggcc gccccagcca atgagagccg aaacttccag gccatagctg tggatcttct
     1921 tcaggatctc cttgcgctcg gcgctcgtct tgtcttgaag agcgagacca ccgccatacg
     1981 cgctgatgtg taccccatcg acaccgagtt cccgcgcgat ctccatcccg cggaacgggt
     2041 cggatcctcc ggttgtggga atgatggcga tcttcatgcc gtgttcctcc gttcatcggc
     2101 gcggccggtg ccgcgctcag gcgccgggcg cctctcgaga ggaactgttc cagcttcgcc
     2161 gccgggatcc tgccaaacaa tcggccacgc caagcggttt ggcctcggga cgccgtgcta
     2221 cgggagatag tcgaagcacc cggggccaat gcgcggctgc gagggctcct tgagccgggc
     2281 catcacgggg gtgtgaacct ccaggcagtc ctcaccctgc tcccggcgca ggacgatgtt
     2341 gcgcacccag cgggcgttgt ccggctccgg cgcgtccagg cgccagaagg caccgcgcga
     2401 ctcctcgcgg cgcagcgaag cggtgatgat gagtcgcgcc agcgtcagca tgaagccgca
     2461 ctcgagcgcc tcgcgcaacc cggccaccga gctcgcgccc aaatcggcgc gacggctctc
     2521 cagctcagct acttcctccc gcgcctcctc cagggagcgc gctgctttcg tcagcccgca
     2581 cttgcgccac atcagcgagc gcagtccggc ggtcacctcg gcggcaggca cgcttccgga
     2641 gagcagagcc tccaggcgcg cgcgctcctg atccgttctg gttgcgtccc accgggggcg
     2701 gggtgtgctc gtcgcgaagg cagcagcatt ctcgccagca atcgccccga agacaagcgt
     2761 atccgccagg gcagagcctc ccaggcggcc cgcgccgaac agcccgccgg ccgcctcgcc
     2821 ggcagcatag aggcccggca ccgtcgcacg cccgcgctcg tcgatgcgga ttcccccgag
     2881 gaaatagtgc gcgccgcctg cggagaggaa tggctcgggg tcacgcccgc cagagtggag
     2941 gagctcctcg aaacgcgagc ggggaaaagt gaagctgacg cactccaggt cgaccagctc
     3001 ggcgcctgcc tcat
//
LOCUS       DAOSCV010000556         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21200_length_2994_cov_2.344335, whole genome shotgun sequence.
ACCESSION   DAOSCV010000556 DAOSCV010000000
VERSION     DAOSCV010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21200_length_2994_cov_2.344335"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..400)
                     /locus_tag="PLY56_13130"
     CDS             complement(56..400)
                     /locus_tag="PLY56_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006407487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22468.1"
                     /translation="MSSSVLEEELVAQIRRAGLPEPEREVRFCDTRRWRFDLAWPAAG
                     LAVEVDGGVWARGRHNRGSGFIADAEKYNTGQLLGWVILRYTDREIRDGSACREIAEA
                     LRIRAGAGPTDS"
     gene            complement(449..994)
                     /locus_tag="PLY56_13135"
     CDS             complement(449..994)
                     /locus_tag="PLY56_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22469.1"
                     /translation="MSIQPPIKGGQEIWRDPRPDLKGDSAAWSRLLTMARRDYGLHVA
                     STLHWFRTMGTRLQTAGERLVLAGEPGAACTWETFNHWPELADAWRRKIEESGLNGKV
                     CCGWTSLDDYKLAREQWLRPMADKLSDLLRRLAASAEGGMLLRKQASPPSGERRHEQL
                     GKQASLPLGEQRHDGKVTYVL"
     gene            complement(1337..>2994)
                     /locus_tag="PLY56_13140"
     CDS             complement(1337..>2994)
                     /locus_tag="PLY56_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOJ22470.1"
                     /translation="QADRIFRNSGRMREKWDRRHSSDGRTYGQMTLDRAMEGRTAFYR
                     GGDGGEHSIPDAGDEFEVASNAPLGIDGSKPTPDLRQFTFDDLGNARRLIAQHGENVR
                     FVPEWKSWLVWNDRQWRRDTDGEIFRLAAMTVDATREQLDAWQTDNEGLQKAIQKHRS
                     SMGSRRSIEAMERLASSLPGVPVHPDQLDANLDYLPCANGVVDLRTGELLPHSRKHMQ
                     TKCLSTPYVQHAQCPTWDRFFAAVFRDDQEMIDYVWRVLGYTLSGRVTARCFFILHGE
                     GGKNGKSTLVETWGRILEQDVFSSALDVRVLMEQRYQQANQPELATLPGIRFVYTSEA
                     SRNDRFDVAKLKLWTGGDTLEACQKHERPFTFRPQFKLLIATNYRPEVGDIGPAMWDR
                     VHLWPFLQRFSSDPEDLAAGSLPEDTEIGEKLLAEAPGALARAVRGAIEFYEGGLRAA
                     PSVIAAVKEYQRESDHVGQFVEECCDVGEGLEAPMAALYARYRAWCDAGGRKPENLTA
                     FGRVLSLKHFEKRKTMGNIFRVGLALRPSVAEESSRDDSWADI"
BASE COUNT          487 a         1054 c          860 g          593 t
ORIGIN      
        1 gtgcgttaac gaacttcatg gcgcaaccgt ggcagcgcgg cggcttacgg cgcactcagg
       61 aatctgtcgg gccggcaccg gcccggatgc gcagggcctc ggcgatctcc cggcacgccg
      121 agccgtcacg gatctcgcgg tcggtgtagc gcagtatcac ccaacctagg agctgcccgg
      181 tgttgtactt ctccgcgtct gcgatgaagc cggacccccg gttgtgccgg cccctggccc
      241 acacgccgcc gtctacctcc accgccagcc cggcggcagg ccacgccaga tcgaaacgcc
      301 agcgccgcgt gtcgcagaag cgcacctcgc gctctggctc cggcaggccg gcacggcgaa
      361 tctgggcgac gagctcctcc tccaggacgc tgctgctcac aatgctgcct ccagcttcat
      421 gagaacctcc gtgaggctgc cggtgtcgtc acaagacata cgttaccttc ccgtcgtggc
      481 gctgctcgcc caaggggagc gatgcttgct tccccaactg ctcgtgccgc cgctcgcccg
      541 aggggggcga tgcctgcttc cgcaacagca tcccaccctc ggccgaggct gccagtcgcc
      601 tcagcaggtc gctcaacttg tccgccatcg gccgcaacca ctgttcgcgc gccaacttgt
      661 agtcatccag ggatgtccag ccgcagcaca ctttgccgtt gaggccgctc tcctcgatct
      721 tgcgccgcca ggcgtccgcc agctcgggcc agtggttgaa cgtctcccac gtgcacgccg
      781 cgcccggctc cccggccagc accagacgct cgccggccgt ctgcaaccgc gtgcccatcg
      841 tgcggaacca gtgcagcgtc gaagccacgt gcagcccgta gtctcgccgg gccatcgtca
      901 gcagccgcga ccacgcggcg ctgtcgccct tcaggtccgg gcgcgggtcc cgccagatct
      961 cctggccacc cttgatagga ggttggatgc tcatacaaag tcctcccccg ggctcggctc
     1021 ttgaccgcag tccgggcatt cgtactccca ctcattgcca cgcttgcggc tgcgccagcc
     1081 cgcgtcgcgc atctcctgca tcatcttgcc acagcttgct gttgtagaag gtgccgttca
     1141 cgtcgcctct gcctagggat agatggacag tagggagagt tttctgtact gtagctctga
     1201 atcgatttta gtactgtttt tcccatcatg cattatggtt gcaaaactat ccctactgtc
     1261 tctaagccgc gtcaccgctt cattcggcac tccctgaagt atccccgaac tatccccaag
     1321 cgctgagtaa ctatccctag atgtctgccc agctatcgtc tcgggagctt tcctctgcca
     1381 cgcttggtcg tagggcgagg ccaacgcgga aaatgttgcc catcgtcttg cgcttctcga
     1441 agtgcttcag ggatagtacc ctcccgaatg ccgtcaggtt ctccggcttc cgccctccgg
     1501 catcacacca ggctctgtag cgcgcataca gggccgccat gggggcttcc aggccttctc
     1561 ctacatcgca gcattcctcc acgaactggc ccacgtgatc gctctcgcgt tgatactcct
     1621 tgacagcagc tatcacggag ggggctgccc gcagtccgcc ctcgtagaac tctatggccc
     1681 cgcggacggc acgcgcgaga gctccgggcg cttcggcaag cagcttttcg ccgatctccg
     1741 tatcttcagg gagagacccg gcggccaggt cttccgggtc cgagctgaac cgctgcagga
     1801 acggccacag gtgaacgcga tcccacatgg ctgggccgat atcgccgacc tccggccggt
     1861 agttcgtcgc gatcagaagc ttgaactggg gccggaaggt gaaagggcgc tcgtgtttct
     1921 ggcaggcctc tagggtgtca ccgccggtcc atagcttcag cttcgccacg tcaaatcggt
     1981 cgttccggct tgcctcactg gtgtacacga aacgaatacc gggaagcgtc gccagctccg
     2041 gctggttggc ctgttggtat cgctgctcca tcagcacccg cacgtccaaa gccgacgaga
     2101 acacatcctg ctcgagaatg cgcccccacg tctccaccag agtgctcttt ccgttcttgc
     2161 ccccttcgcc gtgcagaatg aagaagcacc gagccgtcac gcgcccgctg agggtgtatc
     2221 ccaggaccct ccagacataa tcaatcatct cctgatcgtc gcggaacacc gccgcgaaga
     2281 atcggtccca ggttgggcat tgcgcatgct gaacgtaggg ggtgctcagg cacttcgtct
     2341 gcatgtgctt gcgcgagtgg ggcagcagct cgccggtgcg aaggtcgaca acgccattag
     2401 cgcacggcag gtagtccagg ttggcatcca gctggtctgg gtggaccgga accccgggga
     2461 gggaagaggc taggcgttcc atcgcctcaa tcgagcgccg gctgcccatc gacgagcggt
     2521 gcttctggat cgccttctgt aggccttcgt tgtctgtctg ccatgcgtca agctgctcgc
     2581 gcgttgcatc taccgtcatc gccgccaggc ggaatatctc tccgtccgta tccctgcgcc
     2641 actgcctgtc attccagacc aaccacgact tccactcggg cacaaaccgc acgttctccc
     2701 cgtgttgtgc tatcaaccgc cgtgcgttgc caaggtcatc gaaggtgaat tgtcgcaagt
     2761 ccggtgtcgg cttacttccg tcgatgccga ggggggcatt gctcgccacc tcgaactcat
     2821 cccccgcatc cgggatcgag tgctcgccgc cgtcgccgcc gcggtagaac gccgtcctcc
     2881 cttccatcgc gcggtccagc gtcatctgcc cgtacgtgcg cccgtccgag gagtgccggc
     2941 ggtcccactt ctcgcgcatc ctccccgagt tgcggaagat gcggtccgct tgcg
//
LOCUS       DAOSCV010000557         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21229_length_2991_cov_3.559946, whole genome shotgun sequence.
ACCESSION   DAOSCV010000557 DAOSCV010000000
VERSION     DAOSCV010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_2991_cov_3.559946"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLY56_13145"
     CDS             <1..578
                     /locus_tag="PLY56_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOJ22471.1"
                     /translation="QGAEATAEILARHGLAIISGGGPGIMEATNRGAQKGGALSVGCN
                     IELPFEQKPNDYQDVTLNFHYFFCRKTVFLKYSSAFVIFPGGFGTMDELFEAATLVQC
                     RKVKHFPIVLFGSDYWGGLLKWMRETMEQREHCIGPHDLDLLQLTDDTEEAARFVLQG
                     IEEMKQRQQKIAASHGLEASQLLPGRVLPKW"
     gene            complement(688..2043)
                     /locus_tag="PLY56_13150"
     CDS             complement(688..2043)
                     /locus_tag="PLY56_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22472.1"
                     /translation="MERVTATGAGKGAIEIGDRLQLFVDSYLIDRAVNTELRLHSPVP
                     REVSFPFDLPWEGRHSFYVTVAKEGGLYRAYYRGAGNPGMPEVTCYAESRDGIAWSKP
                     ELGLFEIQGSRANNIVWMGRGCHNFTPFLDTNPAALPEHRWKALASAGPRDSLVPFVS
                     ADGIRWRMLREEPVITQGAFDSQNVAFWDSARGRYAAYFRGFREGVRAVLMATSDDFI
                     HWTEPQWIDLGETPAEHFYTNATTPYFRAPELLLAFPKRFVPDRQWDPGHGEPGLSDA
                     VFLTSRDGLHFDRTFMEAFIRPGHDPRNWTDRNIGVAFGVVPTSPEEISLYYVEHYRH
                     PSCRLRRATLRVDGFASVRAGYAEGEVVTRPLLFSGGRLAINYATSAAGNVQVELQDE
                     AGRAIPGFTLGECPEIYGDELERLVRWNGTSDVSALAGKKVRLRVRLKDADLYAFRFA
                     A"
     gene            complement(2185..>2991)
                     /locus_tag="PLY56_13155"
     CDS             complement(2185..>2991)
                     /locus_tag="PLY56_13155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ22473.1"
                     /translation="AGHQVTILTTGSQGEPMSALSLMASEEHKKVQIQPGDTVIISAT
                     PIPGNEDLVNRTINHLFRLGADVVYHPLAPVHVSGHAYQDEQKMMYNLVRPRYVLPFH
                     GERRHVAQYARLVESLGHPADRVLKMEPGDILELSDGSATIAGHIDGGPIMVDGLGIG
                     DVSHIVLRDRQLLAQDGILVAVITVERDTGKVIAGPEIVSRGFVYMQESEPLIEEAKL
                     RIRADLESLQESEGVPDWGTVKADVRSSLSRFFYAVTGRQPMIIPIVMEA"
BASE COUNT          541 a          998 c          947 g          505 t
ORIGIN      
        1 atcagggggc ggaggcaacc gccgagattc tggcccggca tgggctggcg atcatctctg
       61 gaggcggccc cggcatcatg gaagccacga atcgaggagc ccagaagggc ggcgcgcttt
      121 cggtcggctg caacatcgag ctccccttcg agcagaagcc gaacgactac caggacgtga
      181 cgctcaattt ccactacttc ttctgccgca agaccgtctt cctgaagtac tcctctgcct
      241 tcgtcatctt cccgggtggc tttggcacga tggacgagct gttcgaggcg gccacgctgg
      301 tacagtgtcg caaggtgaaa cacttcccga tcgtcctctt cggaagcgac tactggggcg
      361 gcctgttgaa gtggatgcgc gagacgatgg agcagcgcga gcactgcatc ggcccacacg
      421 acctggatct gctgcagttg accgacgata cggaagaggc agcgcgcttc gtgctgcaag
      481 gcattgagga gatgaagcag cgccagcaga agattgccgc cagccacggc ctggaggcat
      541 cacagctcct gcctggccgc gtgctcccga aatggtgatc gcgatgggcc gcgcaggccg
      601 gtattcaggg cgatgcattc gccggggaac caccccacgc gctcgctccc aggcgaatgc
      661 ttcgccgcgc gacgaagcat ccccaggtca cgcggcgaac cggaacgcat agagatccgc
      721 gtccttcagg cgcacgcgca agcgcacctt cttcccggcg agggcgctca catcactcgt
      781 gccattccat cgcaccaggc gctccagctc gtcgccgtag atctcagggc actcccccag
      841 cgtgaagccg ggaatcgcgc gtccggcctc atcctgtagc tcgacttgca cgttgcctgc
      901 ggcagaagtg gcgtagttga tcgccagtcg tcccccagaa aagagcagcg gccgcgtcac
      961 cacctcgccc tccgcgtagc cggcgcgcac cgaggcgaag ccatccacgc gcagcgtcgc
     1021 ccggcgcagc cggcagctcg gatgccggta gtgctctacg tagtagagcg aaatctcctc
     1081 cggcgaggtg ggcaccacgc caaatgccac cccgatgttc cggtcggtcc agttccgcgg
     1141 gtcgtgtccg ggccggatga acgcctccat gaaggtgcgg tcaaagtgga gcccgtctcg
     1201 gctggtgagg aagaccgcgt ccgagagtcc aggctctcca tgcccgggat cccactggcg
     1261 gtctggaacg aagcgctttg gaaaggcgag gaggagctcc ggcgcgcgga agtacggcgt
     1321 cgtggcgttg gtgtagaagt gctctgccgg cgtctctccc agatcaatcc attgcggctc
     1381 cgtccaatga atgaagtcgt cggaagtggc catgagcacg gcgcgcactc cctcgcgaaa
     1441 gccgcggaaa taggcagcat agcggccgcg cgcactgtcc cagaaggcga cgttctgcga
     1501 gtcgaaggcg ccctgggtga tcaccggctc ctcgcgcagc atccgccagc ggatcccgtc
     1561 cgcggagacg aagggaacca gcgagtcccg agggccggcg ctggcgagcg ccttccagcg
     1621 gtgctccggc aaggccgcgg gattggtatc caggaaggga gtgaagttgt ggcatccccg
     1681 gcccatccag acgatgttgt tcgcccgaga gccctggatc tcgaacagcc cgagctccgg
     1741 cttgctccag gcgatgccat cccggctttc ggcatagcag gtcacttccg gcatgcccgg
     1801 gttgccggcg ccccggtagt aagctcggta gagaccgccc tccttcgcca cggtcacata
     1861 gaagctgtgg cggccttccc aagggaggtc aaacgggaag gagacctcgc gcggcaccgg
     1921 agagtgaagc cgcagctccg tgttcacggc gcgatcgatc aggtaagaat cgacgaagag
     1981 ctgcaggcga tcgccaatct ctatcgcgcc cttcccggca ccggtggcag tgacccgttc
     2041 catcggcttc ctccttacct cggacgccct cgaggcggtg ctcgaacttc cgcccggcag
     2101 ccgccactcg tggccggcgc ccgcccatgc ccccggtgtt cgcctgggat gcgcctattc
     2161 ctcctgtggc gcgaagcggt agggctatgc ctccatgaca atcgggatga tcatcggctg
     2221 ccgtcccgtc acggcataga agaagcggct gagcgaagag cgcacatctg ctttgaccgt
     2281 gccccagtcg ggcacgccct cgctctcctg cagagactcg agatcggcgc ggatccgcag
     2341 cttcgcctcc tcgatcaacg gctccgactc ctgcatgtag acgaaaccac gcgagacgat
     2401 ctccgggcct gcgatcacct tgccggtatc gcgctcgacc gtgatcacgg cgacgaggat
     2461 cccatcctgc gccaggagct ggcggtcgcg gagaacgatg tggctgacat cgccaatccc
     2521 aagcccatcc accatgatcg gaccgccatc gatatgcccg gcgatggtgg cgctcccgtc
     2581 gctcagctcc aggatatccc ccggctccat cttgagcacg cgatcggcag ggtgcccgag
     2641 cgattccacc aggcgcgcat attgagccac gtgccggcgc tcaccgtgga atgggagcac
     2701 gtagcggggg cgcaccaggt tgtacatcat cttctgctca tcctggtagg cgtgcccgga
     2761 aacgtggacc ggcgccaggg gatggtagac tacatccgcg cccaatcgga agaggtggtt
     2821 gatcgtgcgg ttcaccaggt cctcgttccc cggaatcggc gtagccgaga tgatcaccgt
     2881 atcgccgggc tggatctgca ccttcttgtg ctcctccgaa gccatcaacg agagcgctga
     2941 catcggctcg ccctggctcc ccgtcgtcag gatggtaacc tgatggccgg c
//
LOCUS       DAOSCV010000558         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21264_length_2988_cov_3.859189, whole genome shotgun sequence.
ACCESSION   DAOSCV010000558 DAOSCV010000000
VERSION     DAOSCV010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21264_length_2988_cov_3.859189"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLY56_13160"
     CDS             complement(<1..263)
                     /locus_tag="PLY56_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22474.1"
                     /translation="MGTLHHVLWMSLVAAEEATHEAGAAAHGGGDSILAQLGIQFPLI
                     IAQAVGFLALLFILRAFLYRPVLGMLSTREEEIRARYNDADEA"
     gene            complement(466..738)
                     /gene="atpE"
                     /locus_tag="PLY56_13165"
     CDS             complement(466..738)
                     /gene="atpE"
                     /locus_tag="PLY56_13165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ22475.1"
                     /translation="MLYGAMLALAAGLGLPIAVISAALGQSRAAAAALEGIARQPEAA
                     GKIQTAMIIGLALIESLVIYALLMFFMLQGKLPAVMQVIQATTGAG"
     gene            complement(972..1640)
                     /gene="atpB"
                     /locus_tag="PLY56_13170"
     CDS             complement(972..1640)
                     /gene="atpB"
                     /locus_tag="PLY56_13170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOJ22476.1"
                     /translation="MESHFAPWVILLWSGVIIAFLGTISYLGTRKMRPVPGRLQNALE
                     YIVSTLNEFIKGIIGPEGEKHTPFVGTLFLFILFNNLIGLVPGAMAPTSSLNTTVALA
                     LITFFYVQYHGIRAHGLVGRLKHLAGPIPAMAPLMLPIEIIGELARPLSLSMRLFGNI
                     FGEEQVIVILAGLAYYVLPFVPIPYQLPMMVFGLFTGFVQALVFTMLACVYISLAAGE
                     AEAH"
     gene            complement(1671..2033)
                     /locus_tag="PLY56_13175"
     CDS             complement(1671..2033)
                     /locus_tag="PLY56_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22477.1"
                     /translation="MLLLVVLFTTGLGAFRVAWSIVAGAALGLLVLRGFDWAVRALFV
                     PERTATKGTIAKLLILKLPAMALLLYLVVNSGWFHLPAFAGGVALIQAVILLKAFGIY
                     LMEREEERCSQPGGTSAR"
     gene            complement(2080..2316)
                     /locus_tag="PLY56_13180"
     CDS             complement(2080..2316)
                     /locus_tag="PLY56_13180"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ22478.1"
                     /translation="MRPNRLGDSERKWIRNMALAGQVGIFLAAAIFIGWALGQWLDRK
                     LGTDPWLTALFTLLGAAAGFLELFRVAAQISGDE"
     gene            complement(2327..>2988)
                     /locus_tag="PLY56_13185"
     CDS             complement(2327..>2988)
                     /locus_tag="PLY56_13185"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ22479.1"
                     /translation="MEYGIPLWVKGTETAEPGTQIAGSAAELPHRVTAIAHMPRVAYL
                     ALRVPYREDRRRLELELFKLMARVRVGFHLLRGSPGVVGFAVAHESLNRVADLLDGLV
                     LPIAREGGGTRLYLLRVGQGGSRSEVQSALLSASGTSEEVVMADAETVETCAIVSVIA
                     TRVWETPGMGVAVLARLRDVGVRVLQVADSPLSLSCLVHENDTQRAVQALHAQFIAGA
                     A"
BASE COUNT          619 a         1040 c          848 g          481 t
ORIGIN      
        1 ctggcctcat cggcatcatt atagcgcgcg cggatctcct cctcgcgcgt gctcaacatg
       61 ccaaggaccg ggcgatagag gaatgcgcgg aggatgaaga gcagggcgag gaaaccgacc
      121 gcctgggcaa taatcagcgg aaactggatg cccaactggg cgaggatact atcgcccccg
      181 ccgtgcgcgg ccgctcccgc ctcgtgggtg gcttcttccg ccgcgaccag gctcatccac
      241 aatacatgat gtagcgtacc catgcgcccg ttccatctcc atccggtgtc tcaattggtg
      301 ccgggctttc tcgcccggcg cgaagtggat caacacaatg cgcaagcgcg ccggttccag
      361 tctcccgcca cggcgcgcaa gacgggttcg gggaagcgga tcacccgcct ctcccatcac
      421 aggccccgaa cccaccttac gttgcacgca ctgcgatttc gattcctagc ccgctccggt
      481 ggtggcctgg atgacctgca tcaccgccgg cagcttgccc tgaagcatga agaacatcag
      541 cagagcgtag ataaccagcg actcgatgag cgccaggccg atgatcatgg ccgtctgaat
      601 cttgcctgcc gcttccggct ggcgagcaat tccctcgagg gcggccgccg cagcgcggct
      661 ctgacccaag gcggcactga tcaccgcgat cggcaggccc agacccgcgg ccagcgccag
      721 catggctccg tatagcatta tcggatctcc cttccccatg cggtgcatgc ctgttgtgac
      781 gcaccggcat ccgcagcgcg tcgcgcgcgc tgcaacttta tgactatccg cctccgcgcg
      841 ccctcccatt tgggcgcgct tcgcccgctc gttctccctt tgcaaacgcc gcccacccag
      901 ccaaccctgc gcccacacac gccactgcca cgaaatgggg atcgtgatgc gagggtgacg
      961 gggagagcag gctaatgcgc ttcagcctcg cccgcggcga gcgagatata gacgcaggcc
     1021 agcatcgtga agaccaacgc ctgaacaaag cccgtgaaaa gcccgaacac catcatcggc
     1081 agctgatatg ggatcggcac gaacggcaac acataataag caagcccggc caggatgacg
     1141 atcacctgct cctcgccaaa gatgttcccg aagagacgca tggagagcga gagcggccgg
     1201 gccaactcgc cgataatttc gatggggagc atgagcgggg ccatcgccgg gatcggtccg
     1261 gcaaggtgct tcaggcgccc caccaatccg tgggcacgga taccgtggta ctgcacgtag
     1321 aagaaggtga tcagcgccag ggccaccgtg gtgttcaggc tgctcgtcgg cgccatcgcc
     1381 cccggcacga gcccgatcag gttattaaac aggatgaaga gaaagagcgt gcccacgaat
     1441 ggggtgtgct tctctccctc tggcccgata atccccttga tgaattcatt gagcgtgcta
     1501 acgatatact ccagggcgtt ctgtagacgc ccgggcacgg gcctcatttt tcgcgtaccc
     1561 aggtaggaga tcgtaccaag gaaggctatg atgacacccg accagaggag tatgacccac
     1621 ggggcgaaat gcgattccac gatccggtcc ttaccaacac cgcgacacca tcatcttgcc
     1681 gaggttcctc cgggctggct gcaacgctcc tcttcgcgtt ccatcaaata gatgccgaag
     1741 gctttcagca agatcacggc ttggatgagc gccacccccc cagcgaacgc gggaagatgg
     1801 aaccatccgg agttcactac taggtagagc aacagggcca ttgcaggcag cttgaggatc
     1861 aagagcttgg caatcgtccc cttcgtggcc gttctctctg gcacgaagag agcgcgtacc
     1921 gcccaatcaa agccgcgcaa cacaagcagt cccagtgccg cacctgccac aatgctccac
     1981 gcgacgcgga aagcacccaa tccggtcgtg aagagcacca ccaacaagag cacccacccg
     2041 ctcgtgcggt aggtcctcct aataaatcct tcgtccaatc tactcgtccc ctgaaatttg
     2101 cgccgccact cggaacaact ccaaaaaccc agccgcggcg cccaacagcg taaagagcgc
     2161 ggtcaaccag gggtccgtgc ccaatttccg atccagccac tggcctaggg cccagccgat
     2221 gaagatcgcg gccgccagga agatccccac ctgcccggcg agcgccatgt tgcgaatcca
     2281 cttgcgttcg ctatcaccca atcggttagg ccgcatgccg tccctctcag gccgctccgg
     2341 cgataaactg ggcgtgcagc gcctgaactg cgcgctgcgt atcgttctca tgcaccaggc
     2401 aggagagcga gagggggcta tctgccacct ggaggacgcg tacgccaacg tcgcgaagcc
     2461 tcgcgagcac cgccacaccc atgccggggg tctcccacac gcgggtcgca atcaccgaga
     2521 cgatggcgca cgtctccacc gtctccgcgt ccgccattac cacttcctcg gaagttccgg
     2581 aggcggaaag gagcgcgctt tgaacctccg agcgcgagcc accctgcccc acgcgcagca
     2641 gatagagccg cgttccacca ccttcccggg cgatgggcaa gaccaggcca tccagcagat
     2701 cggccacccg attcagcgac tcgtgcgcca ccgcgaagcc caccacccct gggcttcccc
     2761 gcagcaggtg gaaccccacg cgcacgcgcg ccatcagctt gaacagctcc agctccagac
     2821 gccgccggtc ctcgcggtaa ggcacgcgca acgccaggta ggcaacgcgc ggcatgtgcg
     2881 caatcgccgt cacccgatgg ggtagctctg ccgccgagcc ggcaatctgg gtgcccggct
     2941 ccgcggtctc cgtgcccttc acccagagcg gtatcccata ctccatcg
//
LOCUS       DAOSCV010000559         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21288_length_2986_cov_3.100648, whole genome shotgun sequence.
ACCESSION   DAOSCV010000559 DAOSCV010000000
VERSION     DAOSCV010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21288_length_2986_cov_3.100648"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /gene="fusA"
                     /locus_tag="PLY56_13190"
     CDS             <1..1508
                     /gene="fusA"
                     /locus_tag="PLY56_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ22480.1"
                     /translation="ESEQTEVPDALRPLAEEWRQKMIEAIADADDVVMEKYLEGEEIT
                     TEEIRRALRKGTLDNLLVPVLTGSAFKNKGVQPLLDAVVDYLPSPIDVAAVTGTDPKT
                     DQPIARKPDDDEPFTALAFKIMADPYVGRLTFFRVYSGVMKKGSYVYNANRGRRERLG
                     RILRMHANHREDVDAVSSGDIAAAVGLGDTATGDTLCTEDAPILLEAIQFPEPVIHIA
                     IEPKTKADQDKMAVALERLTNEDPTLKTRTDTETGQTVVSGMGELHLEIIRDRLLREF
                     KVDANVGKPQVAYKEAIRKKVQAEGRYVRQTGGRGQYGHVWLELEPMEPGSGFEFVNK
                     IVGGVVPKEYIPAVEAGVKEALETGVTAGYPVIDVRATLYDGSYHEVDSSEMAFKIAA
                     SMAVKNGVMKAQPIIKEPVMAVEVVTPAEYMGDVMGDLNARRGKITGMEPGSGGTQVI
                     RAQVPLAEMFGYATVIRSMTQGRASYAMEPSHYDEVPRTIADEIIAKAQGR"
     gene            1755..2969
                     /gene="tuf"
                     /locus_tag="PLY56_13195"
     CDS             1755..2969
                     /gene="tuf"
                     /locus_tag="PLY56_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ22481.1"
                     /translation="MAKAKFERTKPHVNIGTIGHVDHGKTSLTAAITKVLAVAKLANY
                     QAYDQIDGAPEERERGVTINIYHAEYETEKRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIIVFLNKADMVDDPELLELVELEVRDLLTK
                     YDFPGDDTPIITGSALKVLELPDDATLMRGGNEWSDKIWELMDAVDSYIPEPKRDEDM
                     PFLMPVEDVFTITGRGTVVTGRAERGVLKPGDTVDIVGLRPEIKTTVCTSMEMFRKTL
                     DEVRAGDNAGLLLRGVSRTDVERGMVIAKPKSITPHTKFEGQVYVLTKEEGGRHTQFF
                     SGYRPQFYFRTTDVTGTLTLPEGVDMVMPGDNVTISAELIQPIAMEEGLRFAIREGGR
                     TVASGVVSKILE"
BASE COUNT          597 a          921 c          986 g          482 t
ORIGIN      
        1 ccgagagcga acagaccgag gttccggacg cacttcggcc cctggcagaa gagtggcgcc
       61 agaagatgat cgaggcgatc gcggacgccg atgacgtcgt catggagaag tatctcgagg
      121 gagaggagat caccaccgaa gagattcggc gcgccctccg caagggaacg ctggacaatc
      181 tgcttgtgcc cgtgctcacc gggtcggcgt tcaagaacaa gggcgtgcag cccctgctcg
      241 atgcggtggt cgattatctc ccctccccca tcgacgtcgc ggcggttacc ggcaccgatc
      301 ccaagacgga tcagcccatt gctcggaagc cggatgacga cgagccgttc acggcgcttg
      361 ctttcaagat catggcggat ccgtatgtgg gccgactcac cttcttccgc gtctattcgg
      421 gcgtcatgaa gaaggggtcg tatgtctaca acgccaaccg cggccggcgc gagcgcctgg
      481 ggcgtatcct ccggatgcac gccaaccacc gcgaggatgt ggatgccgtg tcgtctggcg
      541 atatcgcggc tgccgtcggc ttgggtgata ccgccacggg cgatacgctc tgcaccgagg
      601 atgcccccat cctgctggag gcgatccagt tcccagagcc ggtgatccat atcgccattg
      661 agcccaagac aaaggccgac caggataaga tggcggtggc gctggagcgg cttacgaacg
      721 aggatcccac gctcaaaacg cgcaccgata cggagacggg gcaaacggtc gtctcgggga
      781 tgggcgagct ccatctggag attatccgcg accgcctgct gcgcgagttc aaggtggacg
      841 cgaacgtagg caagccgcag gtggcctaca aagaggccat ccggaagaag gtccaggccg
      901 agggtcgcta tgtgcgccaa accggtggtc gcggccaata cgggcacgtc tggctggagc
      961 tggagccgat ggagcccggg agcggcttcg agttcgtgaa taagatcgtg ggtggcgtgg
     1021 tcccgaagga gtacatcccg gcggttgagg cgggcgtgaa ggaagctctc gagacaggcg
     1081 tgacggcggg ctatcccgtg atcgacgtgc gcgccaccct ctacgacggc tcctaccatg
     1141 aggtcgactc gtcggagatg gccttcaaga tcgccgcttc gatggcggtg aagaacggcg
     1201 tgatgaaggc gcagccgatc atcaaggagc cggtgatggc tgttgaggtc gtgacccccg
     1261 cggagtacat gggggatgtc atgggcgacc tcaatgcccg gcgcgggaag atcacgggca
     1321 tggagccagg ttcggggggc acccaggtaa tccgtgccca ggtgccgttg gccgagatgt
     1381 ttggctacgc gacggtgatc cgctccatga cgcaggggcg cgcctcctat gccatggaac
     1441 cgtcgcatta cgatgaagtg ccccgcacta tcgcggacga gatcatcgcc aaggcacagg
     1501 gaaggtgatg catctctgcc gcgcagacgg caggatgcct gcaattcaga cttcgggctg
     1561 gggaacaggg gcctggcaca ccgatgtagc ccccaccctg ggctcgagaa acacagaggg
     1621 cggcggcccg gcgatcgcct gccaggctgc cggccgaagc caagccgaga cgcgtcgtgc
     1681 gcgcggaccg atccgggcag gaacccgggg gcacgcgccg gccgggccgg caaaaatgag
     1741 gaggagttat aacgatggcg aaggcgaaat ttgagcgaac gaagccgcac gttaacattg
     1801 gtaccattgg ccacgttgac cacgggaaga cttcgctcac cgcagcgatt accaaagtgc
     1861 tggcggttgc caagctggcc aactatcagg cctacgacca gatcgacggt gctcccgagg
     1921 agcgcgagcg cggcgttacg atcaacatct accacgccga gtacgagacc gagaagcgcc
     1981 attatgcgca cgtcgactgc cccggccacg ctgactacat caagaacatg atcaccggcg
     2041 ccgcccagat ggacggcgct atcctggttg tctctgccgc cgacggtccg atgccgcaga
     2101 cgcgcgagca catcctcctg gcccgccagg tcggcgttcc ctacatcatc gtcttcctga
     2161 acaaggcgga tatggtggac gaccccgagc ttctcgaact ggtcgagctc gaggtgcgtg
     2221 acctgctcac caagtacgac ttccccggcg acgatacccc gatcatcacc ggctcggccc
     2281 tcaaggtcct cgagctgccc gatgacgcca ccctgatgcg cggcggcaac gagtggtccg
     2341 acaagatctg ggagctgatg gacgccgttg atagctacat ccccgagccg aagcgcgatg
     2401 aggacatgcc gttcctcatg cccgtcgagg acgtcttcac catcaccgga cgcggcacgg
     2461 ttgtgaccgg tcgtgcagag cgcggcgtgc tcaagccggg cgataccgtc gacatcgtcg
     2521 gcctgcggcc ggagatcaag actactgtct gcacgagcat ggagatgttc cgcaagacgc
     2581 tggatgaggt gcgcgcgggc gacaacgccg gcctcctgct gcgcggcgtg tcgcgcacgg
     2641 acgtggagcg cggtatggtc atcgccaagc ccaagagcat caccccgcac accaagttcg
     2701 aaggccaggt gtacgtgctc acgaaggaag agggcggtcg ccacacccag ttcttctccg
     2761 gctaccggcc gcagttctac ttccgcacga cggacgttac cggcacgctc accctccccg
     2821 agggtgtcga tatggtcatg cccggcgaca acgtcacgat ttcggccgag ctgatccagc
     2881 caatcgccat ggaagagggc cttcgcttcg ccatccgtga gggcggtcgc acggttgcct
     2941 ctggcgtggt gtcgaagatc ctggagtaag aggaaggcgc acgatg
//
LOCUS       DAOSCV010000560         2981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21346_length_2981_cov_3.868421, whole genome shotgun sequence.
ACCESSION   DAOSCV010000560 DAOSCV010000000
VERSION     DAOSCV010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21346_length_2981_cov_3.868421"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLY56_13200"
     CDS             <1..696
                     /locus_tag="PLY56_13200"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HOJ22482.1"
                     /translation="TTVAGLQELLREFRERGGGQFQYQAELSGVIEADRPLISELAGQ
                     ECVHYRMRVDREWEEENWETDARTGRRELRTRRGCDTMASNERSVPFFLRDATGAIRV
                     DPEGADIETTQVVDRFEPGEHPGGQISFGGFSLFLGSSPGGGRRTLGYRFQEWLTPVG
                     HQAYLLGTVRDVAGELRVCRPPEGKSRFLVSLRSEEELVRSIRHAMTWLQVAIAICFL
                     FGLGLILARLVMG"
     gene            859..1425
                     /locus_tag="PLY56_13205"
     CDS             859..1425
                     /locus_tag="PLY56_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ22483.1"
                     /translation="MLIARSKQGDRQAFDQLVTRYSAQIYNFAYRMTNNRDDAEDIYQ
                     EAFLHAFRGIGSFRADSAFSTWLYRIVRNVYLDEMKRRRARPYASLEENIQTEDGSIA
                     RDVQDDAPTPEEAVQINERRRAVRRAIAQLPETQREILILYEMNQHSYEEIAAILGIN
                     VGTVKSRLNRARRSLRDRLLAEKELFDL"
     gene            1515..2372
                     /locus_tag="PLY56_13210"
     CDS             1515..2372
                     /locus_tag="PLY56_13210"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ22484.1"
                     /translation="MCNEARPIDLWGEFMGCQGYQRSFTAYLDGELAQGQREAIERHV
                     QRCAECRRDLETLRQTVRLLRSPLLPMEDAPEEVRARLTQKLRAVRQPMSWWERVRLQ
                     WRVAGAASQARTWGYGLAATALLFVVGASNLDRWNAPANPLHVGVGVSRTEALPRPDG
                     HVAARLPQPVGEGVQIRSFVEYPVVVGRPAIVYLFVEPGRDLPDGEVRLYPAAGLSVG
                     GPGVLQSDASYLLYQGPVTRQNPQSRWIPVELCAAREGTHILRVTVREAGRVLSEVTI
                     PLRVVVPRS"
     gene            2623..>2981
                     /locus_tag="PLY56_13215"
     CDS             2623..>2981
                     /locus_tag="PLY56_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22485.1"
                     /translation="MNYPVWEVPLLGGGMLIAAIAVLHVFISHFAVGGGLFLALTERK
                     AYREGDTELLAYTRKHSTFFVLLTLVLGAVTGVGIWWTIGLVHPTGTSALIHIFVWGW
                     AIEWTFFVVEIAAALFYV"
BASE COUNT          497 a          945 c          981 g          558 t
ORIGIN      
        1 acaacggtcg ccggtctcca ggagctcctg cgcgagtttc gagagcgtgg cggcggccag
       61 ttccagtacc aggccgagct gagcggcgtg atcgaagcag accgcccgtt gatctccgaa
      121 ctggccggcc aggagtgcgt ccactaccgg atgcgcgtgg atcgcgagtg ggaagaggag
      181 aattgggaga cggacgcgcg cacggggagg cgcgaactgc gcacccggcg tggatgcgat
      241 accatggcca gcaacgagcg ctccgtgccg ttcttccttc gcgatgcgac cggcgccatc
      301 cgcgttgatc ccgagggcgc ggatatcgaa acgacccagg tcgtggatcg cttcgagccg
      361 ggcgagcacc cgggaggcca gatctccttc ggcggattct cgctctttct gggaagcagc
      421 cccggcggcg gccggcgcac tctgggatac cgtttccagg aatggctgac acccgtcggt
      481 caccaggcct atctgttggg aaccgtccgc gatgtggcgg gtgagttgcg cgtgtgccgg
      541 cctccagagg ggaagagccg cttcctcgtg agcctgcgtt ccgaagaaga gctggtgcgc
      601 tcgatccgcc acgcgatgac ctggctgcag gtggcgattg ccatttgctt cctctttggt
      661 ctgggcctga ttctggcccg cctggtcatg ggctagagcc ccaaggggga gaagaggaga
      721 gtattgactt gcccctgccc ctgtgttaca atgccgatac cacgaagatt tggactgggg
      781 tagatgatct cgccgcgtga tgctccaagg aagcccgaag gcccctcccg gtctagcctg
      841 gaccagtgcg atgactcggt gctcattgcc cggagcaagc aaggcgaccg ccaggcgttc
      901 gatcaactgg tcacacgcta ctccgctcag atctataact tcgcctatcg gatgaccaac
      961 aaccgagacg atgcggagga tatctaccag gaagcgttcc tccatgcctt ccggggaatt
     1021 ggcagcttcc gggccgactc cgccttttcg acctggttgt accgcatcgt gcgcaacgtc
     1081 tacctcgatg agatgaagcg ccggcgcgcg cgcccctatg cgtccctcga agagaacatt
     1141 cagacggagg atggctcgat tgcccgcgac gtgcaagatg acgccccaac cccggaggag
     1201 gcggttcaga tcaacgagcg ccggcgcgcc gtccgccgcg cgattgccca gctcccggaa
     1261 acacagcgcg agatcctgat cctgtatgag atgaaccagc acagttacga ggagatcgcc
     1321 gccattctcg ggatcaacgt gggcacggtc aagtcgcgcc tcaatcgcgc acgccggagc
     1381 ctgcgagacc ggctgctcgc ggagaaggaa cttttcgatc tctgagctcg tctaaatctg
     1441 tgagcacagt cgagtgagca gcaccctgct gctttctgcc cggttgatgc tggaagtcgc
     1501 gtggcggtcc tgcgttgtgc aacgaggccc ggccgatcga cctgtggggc gagttcatgg
     1561 gatgccaggg ataccaacgg agcttcactg cctatcttga tggcgagttg gctcagggcc
     1621 agcgcgaggc gattgagcgc cacgtgcaga ggtgcgcgga gtgccggcga gatctggaga
     1681 cgttgcgcca gactgttcgc ctgcttcgat cgccactgct gccgatggag gatgcccccg
     1741 aagaagtgcg cgcgcggctg acgcagaaac tgcgtgccgt gcgccagccg atgagctggt
     1801 gggagcgagt gcgcctgcag tggcgtgtcg cgggtgcggc cagccaggca cgcacctggg
     1861 gctatggcct ggcggccacc gccctcctct tcgtggtggg ggccagcaac ctcgaccggt
     1921 ggaacgcgcc cgcgaacccg ttgcacgtcg gcgttggcgt ctcgcgcacc gaggcgcttc
     1981 cccggccgga tggccacgtt gctgccagac ttccgcaacc agtgggcgag ggggtgcaga
     2041 tccgctcgtt tgtggagtac ccggtcgtcg tgggccggcc ggcgattgtc tacctcttcg
     2101 tggagcctgg ccgcgacctc cccgatggcg aagtgcgcct ctacccggcc gccggcctgt
     2161 cggttggcgg gcctggcgtc ctgcagagcg atgcgtccta tctcctctac cagggtccag
     2221 tgacgcgcca gaatccccag agccggtgga ttcccgtgga actgtgcgcc gcccgggagg
     2281 gcacgcacat ccttcgcgtc accgtgcgcg aggccgggcg tgtcctcagc gaagtgacga
     2341 ttccgctcag ggtggtggtc ccgaggagct gaggcccgca gcagccccag gtgggcttcg
     2401 gggatactca tcccaccctc gtttggctcc ggcgcgctct ctccagacct gggaaagcca
     2461 ggtaaccttg cccaacccat gcgggtggtg gtcttccgca gccctcacgc gcgtgcagcc
     2521 cccggtcgct tcaaagccgc atctcccctt gacgaagcta taagcggttg ctaaaatggg
     2581 acttgcaccc cgcatatcga acctgagagg ggatcgaagg gtatgaacta ccctgtctgg
     2641 gaggtgccgc tgcttggggg cggcatgctg atcgcagcga tcgctgttct gcacgtcttc
     2701 atctcccatt ttgcggtcgg cggcgggctt ttccttgccc tgacggagcg caaggcctat
     2761 cgggaggggg acacagagct cctcgcctat acacggaagc actctacctt cttcgtgctc
     2821 ctcacgctcg tcctcggcgc ggtcaccggc gtaggtatct ggtggaccat cggcctggta
     2881 cacccgactg gtacctcggc actgatccac atcttcgtct ggggctgggc gattgaatgg
     2941 accttcttcg tcgtggagat cgccgcagcc ctcttctatg t
//
LOCUS       DAOSCV010000561         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21359_length_2980_cov_3.858803, whole genome shotgun sequence.
ACCESSION   DAOSCV010000561 DAOSCV010000000
VERSION     DAOSCV010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21359_length_2980_cov_3.858803"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLY56_13220"
     CDS             complement(<1..401)
                     /locus_tag="PLY56_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOJ22486.1"
                     /translation="MSTNEKAVQSILATDCGSTTTKAILIEKQGDEYRLVARGEAPTT
                     VEAPFDDVTVGVRNAAAEVEDLTGKRLLEAGRLIVPARDSKTGTDLYLSTSSAGGGLQ
                     MMVAGVVRSMSGESAQRAALGAGAIIMDVIAV"
     gene            581..1018
                     /locus_tag="PLY56_13225"
     CDS             581..1018
                     /locus_tag="PLY56_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ22487.1"
                     /translation="MIIADLKEIAGRTYPARRRTQNLVGGASPIQATNFAIGYVTLEP
                     KGGQVPWHNQEQEEVYFLLDGKAEICLGEERRVLEAGQAVYIPSGVFHQLTNIGDEPA
                     HMMYCYGPAGDVAHWRQELDGTLPKAGVDAPPLPEGAQPQCTD"
     gene            1101..2252
                     /locus_tag="PLY56_13230"
     CDS             1101..2252
                     /locus_tag="PLY56_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22488.1"
                     /translation="MKTHKVGIVMNGVTGRMGTNQHLMRSIVAIIRQGGVKISDEEVI
                     LPDPILVGRNPAKLEALSNTSGVEKWTTDLESALADPHNIIYFDSQTTDRRANAVKRA
                     IAAGKHIYCEKPTALTTEEAYELYTLAKAAGVKHGVVQDKLWLPGLQKLKMLIDTGFF
                     GRILSVRGEFGYWVFEGDSVPAQRPSWNYRREEGGGIIIDMLCHWRYVLDNLFGEVKS
                     VSCLGATHVDTRWDEKGQPYKCTAEDAAYATFELEGGIIAHFNSSWTVRVRRDDLLTI
                     QVDGTKGSAVAGLRECVVQHASVTPRPIWNPDIDSPINYYDAWLPVPAFKPFDNAFKI
                     QWELFLRHVVKDEPFRWNLLEGAKGVQLAEKGMESWQKRAWVELPALKP"
     gene            complement(2326..2682)
                     /locus_tag="PLY56_13235"
     CDS             complement(2326..2682)
                     /locus_tag="PLY56_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22489.1"
                     /translation="MMWRLWAKRLALPAGLVVAMVAYSAFYGGLLRILLGASACALIG
                     AVAGALGGAIPHRSRWGAADGACLGAIIGAGAGARMGLDWLNWRMVATLFLLTGFFGL
                     CMADPPTGEVPASPEK"
     gene            complement(2692..>2980)
                     /locus_tag="PLY56_13240"
     CDS             complement(2692..>2980)
                     /locus_tag="PLY56_13240"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ22490.1"
                     /translation="ESNFTAIHQPPQIDFTLIARGLGVEAARVTDPEEMAKALDRALA
                     HPGPFVLEVETDAAEEVLPMVPPGGSLSEMTYGEESAAPPRGIEEELSGPR"
BASE COUNT          559 a          946 c          981 g          494 t
ORIGIN      
        1 acggcgatca cgtccatgat tatggcgcca gcgccgagtg cggcgcgctg cgcgctttcc
       61 cccgacatcg agcggaccac ccccgcgacc atcatctgca ggccgccacc ggcgctggac
      121 gtggagaggt agaggtcggt gccggttttg ctgtcgcggg caggaacgat gaggcggccc
      181 gcctccagga gccgcttccc cgtgaggtct tccacctcgg cggccgcgtt gcgcacgccc
      241 acggtgacgt cgtcgaaggg cgcctccacc gtcgtcggtg cttcgccccg cgccaccagc
      301 cggtactcat cgccttgctt ctcaatgagg atcgccttgg tggtcgtgct gccgcagtcg
      361 gttgccagga tcgattgtac cgccttttcg ttcgttgaca tggcgatttc attataacat
      421 agccccaaag gcgcgacaat agtgttggcc ggccggtcag caaggttcga gaggcgccag
      481 cgtagaacaa gggaatggcg cggcgccagg cgtcgaggcc gacccgcggg cactcgccac
      541 gcggcgcgtg gcccaatgac cagagcatcg gaggaagcac gtgatcatcg cggatctgaa
      601 ggagatcgct ggacggacgt atccagcgag aagacggaca cagaaccttg tcgggggtgc
      661 ctcgccgatc caggcgacca acttcgccat cggctatgtg accctggagc caaagggtgg
      721 ccaggtgccc tggcacaatc aggagcagga ggaggtctac ttccttctcg atggcaaggc
      781 ggagatctgc ctgggcgaag agcgacgcgt gctcgaagct gggcaggcgg tctacatccc
      841 gtctggggtc ttccaccagc tcaccaacat cggcgacgag ccggcgcata tgatgtactg
      901 ctatgggccc gctggcgacg tggcgcactg gcggcaagaa ctggatggaa ccctgccgaa
      961 ggctggcgtg gacgcgccgc cactcccgga gggcgcgcag ccacagtgca ccgactgaga
     1021 ggcgtgatag ggtcgaggca tggcggattg ctccgtccct ctcacatccc acaccacgga
     1081 ggtaatcagc gttgagcgag atcaagacac acaaggtcgg cattgtcatg aacggcgtga
     1141 cggggcgcat gggcacgaac cagcacctga tgcgctccat cgtggctatc atccggcagg
     1201 gcggggtgaa aatcagcgat gaggaggtca tcctgcctga tcccatcctc gtcgggcgca
     1261 atccggccaa gctggaggcg ctctccaaca cctccggtgt cgagaagtgg accacggatc
     1321 tggagagcgc cctggccgac cctcacaaca tcatctattt tgactcgcag actaccgacc
     1381 gccgtgccaa tgcggtgaag agagcaatcg cggcgggcaa gcacatctac tgcgagaagc
     1441 cgacagcgct caccacggag gaagcctacg agctctatac gctggcgaag gccgcgggcg
     1501 tcaagcacgg cgtggtgcaa gacaagctgt ggctgcccgg gttgcagaag ctgaagatgc
     1561 tgatcgacac cggcttcttc gggcgcatcc tctcggtgcg tggcgagttt ggctactggg
     1621 ttttcgaggg cgactcggta ccggcgcagc ggccctcctg gaactaccgg cgtgaggaag
     1681 gcggcggcat catcatcgat atgctctgcc actggcgcta tgtgctcgat aacctgttcg
     1741 gcgaggtgaa gagcgtctcg tgcctgggcg ccacccatgt ggatacgcgc tgggatgaga
     1801 aggggcaacc ctataagtgt accgccgagg atgccgcgta tgctaccttc gagctcgagg
     1861 gagggatcat cgcgcacttc aactcgtcat ggaccgtgcg cgtccgacgc gatgatctgc
     1921 tgacgatcca ggtggatggc accaagggat cggccgtggc cggcctgcgc gagtgcgtgg
     1981 tgcagcacgc ctcggtgacg ccccgcccga tctggaaccc ggatatcgac agcccgatca
     2041 actactatga tgcctggctc ccggtgccgg cgttcaaacc gttcgataac gccttcaaga
     2101 ttcagtggga gctcttcctg cgccatgtcg tcaaggacga accgttccgc tggaacctgt
     2161 tggagggcgc gaagggcgtg cagcttgccg agaaggggat ggagtcctgg cagaagcgcg
     2221 cctgggtgga gctgcctgcg ctgaagccgt aggagactct ttcaccgctt cgcgacagag
     2281 tgcagtgggg ggtgtccgga gtgatgcggc tccggacacc ccccgtcatt tctcgggaga
     2341 ggcgggcacc tctcccgttg gcggatcggc catgcagagg ccaaagaagc cggtcagaag
     2401 gaagagggta gccaccatcc gccagttgag ccaatccagg cccatgcgcg cgccggcacc
     2461 ggcaccgatg atggctccga ggcaggcacc atcggctgcg ccccacctgg agcggtgagg
     2521 aatggcgccg cccagggcgc ccgcgaccgc gccgatcaga gcgcaggcac tggcgccgag
     2581 caggatccgc agtagccccc cgtagaaggc actgtacgca accatcgcca ccaccaggcc
     2641 cgcgggaagc gcgagccgct tcgcccatag cctccacatc atcttgaccg cctaacgagg
     2701 tccagagagt tcctcctcga tgccacgcgg gggcgcagcg ctctcctcgc cgtaggtcat
     2761 ctccgagagg cttccacccg gtggcaccat cgggagcacc tcctcggcgg cgtccgtttc
     2821 aacttccagg acaaaggggc ccggatgcgc cagcgcccga tccagagcct tggccatctc
     2881 ttccggatcg gtcactcgcg ccgcctcgac gcccagaccg cgggcaatga gcgtgaagtc
     2941 aatctgcggg ggctggtgta tcgccgtaaa gttgctctcg
//
LOCUS       DAOSCV010000562         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21481_length_2968_cov_3.418812, whole genome shotgun sequence.
ACCESSION   DAOSCV010000562 DAOSCV010000000
VERSION     DAOSCV010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21481_length_2968_cov_3.418812"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLY56_13245"
     CDS             <1..942
                     /locus_tag="PLY56_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22491.1"
                     /translation="AGTMEILETGISGVKIDLSNKRNLIILASLAGVAFAAALFFVVF
                     SGGPKVSEEARRAEEAREARGPLPRGAEVEGMPATPDAGGPGPEGDAGAAPEGPEMAA
                     AGPAEPAGGPGEPPAGTPAGPDEGSAPEAQPAPKPFEAEGDGGTIPKGPPVEPYRPDP
                     FVTAAKLEAPRPTPVLPPLVVVRQPPQPMVVPATVNVIPTSIIARGSAAIRAEVQRRL
                     SGVLWNGKVFAILESSDEAQRAIVQPGEQVGKDLWVKSISRSQMVLVREDGERGREEI
                     VVDLKEAQRQPAMGLGPGGMPGMPGDPGAFPGGMPAP"
     gene            1144..2049
                     /locus_tag="PLY56_13250"
     CDS             1144..2049
                     /locus_tag="PLY56_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22492.1"
                     /translation="MRSRRRFGRSWYRRGCSILLSLGLAAGLAALPAQAQENAAQEKI
                     SLDLVDADLRDAVTLIFKDTPLNYTFADGVYATNVTLKLDGVPRERALDILLKSKGLQ
                     YSKEGGVYVIKPRPGANPGGMGVPGGMAVPGGEAMPAGAPGVARPAATGAEAGGGAAA
                     PAGKRESKLEQIKLTHLDPWYLIAVLRGQSNQGGQFGGVGGGGFGSMGSGFGGGMGSF
                     GGGMGSFGGSSFGGGMGSFGGSMGGFGFGSMGGFGGSMGSLGGGLGGLGGGLGSFGGS
                     SFGGGSSGQGQFNTGQQVFRPQLRR"
     gene            2429..>2968
                     /locus_tag="PLY56_13255"
     CDS             2429..>2968
                     /locus_tag="PLY56_13255"
                     /inference="COORDINATES: protein motif:HMM:NF019283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOJ22493.1"
                     /translation="MSAPISIDLRDCEIGAAIRTLMQASGANIIVDGEVSGRVTCQLR
                     DKTLEEVLDLLTQAKGLHWHKKNDVYVITRQPIASPAAAPVAAAEAPPAPPAAPEVTG
                     AIDLNPRSVEAAAPRPKTSLQSIPLRHLDPYAAADLLEKGTMTSSIDLMASMMRESGV
                     PTTGIPQFFQNNTATPAGGN"
BASE COUNT          511 a          903 c         1094 g          460 t
ORIGIN      
        1 gcgggcacca tggagatctt ggagacgggc atcagcggcg tgaagatcga cctgagcaac
       61 aagaggaacc tgatcatcct ggctagtctt gccggggtgg ctttcgccgc ggcgctcttc
      121 tttgtggtct tctccggagg gcccaaggtt tctgaggaag cccgccgggc ggaggaggcg
      181 cgcgaggcgc gcggcccgct cccccggggc gccgaggtgg aggggatgcc cgcgacgccg
      241 gatgcgggcg gcccgggacc agagggcgac gcgggagcag ccccggaggg cccggagatg
      301 gctgcggctg gccccgcaga gccggcagga ggcccgggcg agccaccggc aggaacgccg
      361 gctggtcccg acgaaggatc tgccccggaa gcccagccgg ccccgaagcc gttcgaggcg
      421 gagggcgatg gaggcacgat tccgaagggg ccaccggtgg agccgtatcg gccagatccc
      481 ttcgtgaccg cggccaagct ggaagctccg aggcccacgc cagtgctgcc accgctggtg
      541 gtggtacgcc agccacccca accgatggtg gttccggcga cggtgaacgt gattccgacg
      601 agcattattg cccgcggaag cgcggcgatc cgcgcggaag tccagcgacg cctctctggc
      661 gtgctctgga atggaaaggt cttcgccatc ctggagtcgt ccgacgaggc gcaaagggcg
      721 attgtgcagc ccggcgagca ggtcggcaag gatctttggg tgaagtcgat ctcgcgatcg
      781 cagatggtgc ttgttcgaga ggatggggaa cggggccgcg aggagatcgt ggtcgatctc
      841 aaggaagcgc aaagacaacc ggcgatggga ctcggcccgg gcgggatgcc cggtatgcct
      901 ggcgacccgg gcgcatttcc tggcggaatg cccgctccat agagttcaga gttagttgct
      961 agcgagggtg tgatcggtgc gtgacgcgca gagggagcgc agtctacgcg ccgctgggcc
     1021 aacctcccgg cccattgcga agtggtgtcg tcggttacag ccgttgggcg ccaaaggaaa
     1081 gagatgggag gcgaatcgcg gcggcggggc gctgccgtcg cgagagagga agaggtggga
     1141 acgatgcgta gtcgaaggag attcggcaga tcgtggtaca ggcgggggtg ctcgattctc
     1201 ctgagcctgg ggctggctgc cggcctggct gccctgcctg cgcaggcgca ggagaacgcg
     1261 gcacaagaga agatctcgct cgatcttgtc gatgccgatt tgcgtgacgc ggtcaccctg
     1321 atcttcaaag acacccctct caactacacc ttcgcggatg gtgtgtacgc caccaatgtg
     1381 acgctgaagc tggacggagt gccccgcgag cgggcgctcg atatcctgct gaagagcaag
     1441 gggcttcagt actccaagga ggggggcgtc tacgtcatca agccgcggcc gggcgcgaac
     1501 cccggcggga tgggcgttcc cggaggaatg gccgttccgg gtggcgaggc gatgccggcc
     1561 ggggcacccg gtgtggcgcg tccggcggcg accggcgctg aagccggtgg cggcgccgcg
     1621 gctcccgctg ggaagcgcga gagcaagctg gagcagatca agctgacgca cctcgatcca
     1681 tggtacctca tcgcggtgct ccgtggccag agcaaccagg gcggccagtt tggcggcgtt
     1741 ggcggtggcg gcttcggcag catgggaagc ggcttcggcg gtggcatggg cagcttcggc
     1801 ggcggaatgg gcagcttcgg gggcagcagc ttcggcggtg gcatgggcag cttcggcggc
     1861 agcatgggtg gcttcggctt cggcagcatg ggcggcttcg gcggcagcat gggcagcctt
     1921 ggcggtggcc tgggtggcct cggcggcggt ctgggcagct tcgggggcag cagctttgga
     1981 ggcggcagct ccggccaggg ccagttcaac accggccagc aagtgttccg gccgcagctt
     2041 cgccggtaag tgagtgatga actcgctttc agggcgccaa ccgtggcgcc ctgaaggctc
     2101 ggcggcctac gggcacgccc catacaggtg cgggcgacgt gtcaggtgcg tccggatgac
     2161 aagcgaggaa ctggagaggg caggcgagtc tccccaactc ccggatatgt gccgtggagc
     2221 tgaggctcga gaggtccgct tagggcgcga atcgatcatc attggctcat cgcgggacac
     2281 ccgtcctcca ggttggcagt gagacacgcg caggtacaat atgacagcac ataccgattt
     2341 tctgagaccg gcccataggc cgctgttctg gctcgccgcg ctctttgcca tcattctagt
     2401 ggccccgccg gcaccggcgg aggatgcgat gagcgccccg atttcgatcg atcttcgcga
     2461 ttgtgagatt ggggcggcaa tccgcacgct gatgcaggcg agcggggcga acatcatcgt
     2521 cgacggtgag gtcagcggca gagtgacgtg ccagctccgc gacaagacgc tcgaggaagt
     2581 cctcgatctc ctcacgcagg ccaagggact gcactggcac aagaagaacg atgtgtacgt
     2641 catcacgcgc cagcccatcg ccagtcccgc tgcggcgccc gtcgcggctg cggaagcgcc
     2701 ccccgctccg ccggccgccc ctgaggtcac gggtgcgatc gatctgaacc cgcgctcggt
     2761 cgaggcggct gcgccacgcc cgaaaaccag cctccaatcg atccccctcc gccatctcga
     2821 tccctacgcg gcggcggatc tgctggagaa gggcacgatg acctcgagca tcgacctgat
     2881 ggcatccatg atgcgcgaga gcggcgtgcc tacgactggc atcccgcagt tcttccagaa
     2941 caacaccgca acgcccgcgg gtggcaat
//
LOCUS       DAOSCV010000563         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21533_length_2963_cov_3.003783, whole genome shotgun sequence.
ACCESSION   DAOSCV010000563 DAOSCV010000000
VERSION     DAOSCV010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21533_length_2963_cov_3.003783"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..816)
                     /locus_tag="PLY56_13260"
     CDS             complement(145..816)
                     /locus_tag="PLY56_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22494.1"
                     /translation="MHRERVFDFWLTAAVTVALFIPIWGVLLRPTGLIRLPVYLLYLG
                     VPALAVILGALIYGARSNRVLLNRFLVGYAGGFTATAIFTLLTVGAGPWLDTPNVAVA
                     LGQWVLERPLTAPVTPAVIAVGMAYHFLLNGAAWGAAYALLFGKAPWWLGLLFGFFIW
                     AVLVLSPPFYQWGLAGAASGAWLLIALLLVHFVYGGIVGYIVYRWVFPEVGMEGIKAI
                     RPLYA"
     gene            complement(831..1001)
                     /locus_tag="PLY56_13265"
     CDS             complement(831..1001)
                     /locus_tag="PLY56_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22495.1"
                     /translation="MERKQRPEVPVPEQIERWHHPGPPTVDEVPEEIEEQRLEATRRQ
                     QKMTEDKTGEAE"
     gene            complement(1204..2031)
                     /gene="uppP"
                     /locus_tag="PLY56_13270"
     CDS             complement(1204..2031)
                     /gene="uppP"
                     /locus_tag="PLY56_13270"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOJ22496.1"
                     /translation="MEQSLSILQAIFLGAVQGITEFLPISSSGHLVALPAVLGWKEHS
                     LQFDIALHMGTVLALILYFWKDWASMGLAILGLGPGTPEERRERSRLAWVLMVACVPA
                     AVLGVTIADAAETTLRSPLLVAGMLAGVGVLLLIADRTSRGARTVKDARLLDGVTVGL
                     AQALALVPGVSRSGITMTAGLMRNLSREDAARFSFLLSVPVTLGAGLFGLRDLFLGGL
                     PASDLAPFVAGTVASAVVGYATIAFLLDYVRKHDLRIFVYYRWALAALIVAIHFIRS"
     gene            2310..>2963
                     /locus_tag="PLY56_13275"
     CDS             2310..>2963
                     /locus_tag="PLY56_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ22497.1"
                     /translation="MARKHTVEESWKLWDEANAYIPMATQTHSKAPRAALRGVEPCFV
                     TRGQGCRVWDLDGNEYIDFRNALGPISLGYRYPAVEDAIRRQLENGIIFGYATPLEVE
                     VARHLVEIIPCAQSVRFLKTGGEAMAAAIKLARAFTGRELVLKCGYHGWLQTMTTPGM
                     PEGAAASYRDLPWGDIRPYEEAFAREGDRIAAVTVACAYADIEQGHAFYPALRELTEK
                     "
BASE COUNT          557 a          978 c          989 g          439 t
ORIGIN      
        1 cgtatcggcc agctcgtaag agatcaggcc gtctgtcgcg atccgacgca ccacgccgct
       61 ctccggctgc ccgcttgata cgtggcttcg cacgcgctcc tccacctgca gtgccatcgc
      121 ggctcccttc gcctcgtgcg cgcgtcacgc gtagagtggg cggatcgctt tgatcccctc
      181 catgcccact tctggaaaga cccaacgata gacgatgtag cccacaatgc caccatacac
      241 gaaatgcacc aggaggaggg cgatcaggag ccaggccccg gatgccgcgc cggccaggcc
      301 ccactggtag aacggtgggg agagcacgag cacggcccag atgaagaagc cgaacaggag
      361 gcccagccac cagggggcct tcccgaaaag gagcgcgtag gcagcgcccc acgcggcgcc
      421 gttgagcaag aagtggtagg ccatgccgac cgctatcacg gccggcgtca cgggcgccgt
      481 cagtggccgc tccaggaccc actggcccag cgccaccgca acgttcggag tatccagcca
      541 cggccccgcc ccaacggtga gcagcgtgaa gatggcggtc gccgtaaacc cgcccgcgta
      601 gccgaccagg aagcggttca gcagcacccg gttactccgg gccccataga tcagagcgcc
      661 gaggatgacc gcgagcgccg gcacgcccag gtagaggagg tagacgggca gacggatcaa
      721 cccggtgggc cgcagcaaca cgccccagat cgggatgaac agggcgacgg tgacagcggc
      781 ggtcagccag aaatcgaaga cccgttcgcg gtgcatcagg caggctccgg ctactccgcc
      841 tctccggtct tgtcctcggt catcttctgc tggcgccgtg tcgcctccag acgctgctcc
      901 tcgatctcct ccggcacctc atccaccgtg ggcggtccgg ggtggtgcca gcgctcgatt
      961 tgctctggca ccggcacctc cgggcgttgc ttgcgctcca ttccgctcat cgctcccttc
     1021 gctcatcggg cgccaggacc ccggaggcat gccggggccg ggcggcgcgt tcccatgcag
     1081 cggagctttt ccccgaacga acggcctgga aactcggccc gtgggcgcga accccgatgc
     1141 cgcgcggatg gggcggctgg ctggcggttc gtggaacgcg cgcctgccag gcgcgcgttc
     1201 cattcaggag cggatgaaat ggatggccac aatcagcgcg gcgagcgccc accggtagta
     1261 gacgaagatg cgcagatcat gcttgcggac gtaatcgagg aggaaggcga ttgtggcgta
     1321 gccaacgacg gcggatgcga ccgtgcccgc gacgaaaggc gccaggtcgg atgccgggag
     1381 gccgcccagg aagagatcgc gtagcccaaa aagccccgcg ccaagggtaa ccggcacgga
     1441 gaggaggaag gagaagcgcg ccgcgtcctc ccgcgacagg ttgcgcatca ggcccgcggt
     1501 catcgtgatc cccgagcgcg agacgcccgg caccagcgcg agcgcttgcg cgagccccac
     1561 cgtcacgcca tccaacaggc gcgcatcttt gaccgtgcgc gcgccgcgag aggtgcgatc
     1621 ggcgatgagc aggagaacgc caacgccagc cagcatgccg gcgaccagca gcggcgagcg
     1681 caatgtcgtc tcagccgcgt cggcgatggt cacgcccagg accgcggcgg gaacgcaggc
     1741 gaccatcaag acccatgcga gccgtgaacg ctcgcggcgc tcctccgggg ttccggggcc
     1801 gagaccaaga atggccaggc ccatcgatgc ccagtccttc cagaagtaga ggatgagcgc
     1861 caggacggtg cccatgtgca gcgcgatgtc aaactggagg ctgtgctcct tccagccgag
     1921 cactgccggg agagcaacaa gatgtccaga gctgctgatc ggaagaaact cggtgattcc
     1981 ctgaacggct ccaagaaaga ttgcctgaag gatgctcaac gattgctcca tgccacgatc
     2041 atccgggctc cctccacagg gcccgggggt tgcccccggc gcgtcgcccg gaaatcgatg
     2101 cttgcttgtg gttcgaaccc catcatatca cacccggtct cttcccgcaa gatcctcccc
     2161 acacgttcca gaaggtattc gcagcgtcat tggccatccg gggcgacctc aaaagaggaa
     2221 tgcggcaggc ggcgcggaag cgagagagtg ttggcgcggc gctctttgcg cgagcaggac
     2281 cgcgctaccg tcggagagga gcagccgaag tggcccggaa gcatacggtt gaagaatctt
     2341 ggaagctgtg ggatgaggcg aacgcctaca tccccatggc gacgcagacc cactccaagg
     2401 cgccacgagc cgccttgcgc ggcgtggaac cttgcttcgt gacgcgcggg caggggtgcc
     2461 gtgtttggga tttggacggc aacgagtata tcgattttcg gaacgccctc ggacctatct
     2521 cgctgggcta ccgctatccg gcggttgagg atgcgatccg gcggcagttg gagaacggaa
     2581 ttatctttgg gtacgcgacg ccgctggagg tggaagtcgc ccggcatctg gtggagatca
     2641 tcccctgcgc gcagagcgtt cgctttctga agacgggcgg cgaggcgatg gccgccgcga
     2701 tcaagctcgc gcgcgctttc accgggcgcg agctggtcct caagtgcggt taccacggct
     2761 ggttgcagac gatgaccact ccgggaatgc ccgagggcgc cgccgcatcc tatcgggatc
     2821 ttccctgggg cgatatccgg ccttacgagg aggcgttcgc gcgcgagggc gatcgcatcg
     2881 ccgccgtcac ggtcgcatgc gcttacgcgg atatcgagca gggccatgcc ttctacccgg
     2941 cgctgcgcga gctgaccgag aag
//
LOCUS       DAOSCV010000564         2960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21565_length_2960_cov_2.827194, whole genome shotgun sequence.
ACCESSION   DAOSCV010000564 DAOSCV010000000
VERSION     DAOSCV010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21565_length_2960_cov_2.827194"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1732
                     /gene="glnA"
                     /locus_tag="PLY56_13280"
     CDS             314..1732
                     /gene="glnA"
                     /locus_tag="PLY56_13280"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOJ22498.1"
                     /translation="MKTPGEVIAFARGNGIRVVDFRFVDLPGIWQHFSIPVRHLTEEL
                     FENGIGFDGSSIRGFKEIHESDMILIPDARTAFVDAFTQEPTLNILCDVHDPITRQPY
                     TRDPRYIARRAEEYLGSTGIADTAYFGPEAEFYIFDDVRFDSTTHSAFYAVDSSEAAW
                     NTGRDERPNLGYKIRYKEGYFPVPPMDQFMDLRTEMMLELEKAGIETELHHHEVATAG
                     QAEIDVRYDTLLATADKLMKFKYVVKNVARRNNRTVTFMPKPLFQDNGSGMHVHQSLW
                     KDGRNLFWDERGYAGLSEIAIYYIGGLLKHAPSILAFAAPTTNSYRRLVPGYEAPINL
                     IYSQRNRSACVRIPVYSKSPNAKRIEFRPPDPSSNPYLCFAALLMAGLDGIQNRIMPP
                     EPIDKDLYDLPPEEKAAVESTPASLADALDALEADHDFLLKGDVFTEDAIRAWIDFKR
                     KREVEQVALRPHPHEFALYYDI"
     gene            2127..2900
                     /locus_tag="PLY56_13285"
     CDS             2127..2900
                     /locus_tag="PLY56_13285"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOJ22499.1"
                     /translation="MAKIISIANQKGGVAKTTTTVNLGAALAEAHQRVLLIDLDPQAH
                     LALSLGLNPDDLDASTFNVLAEPSVGFPRIITPISEGLALAPADINLSVAEQRLGGTI
                     GREQILRDKLREVQADYDYILIDCPPSLGVLTVNALAASQRVIIPVQAHYLSMKGMQL
                     LIETIGLVREKLNPQLEILGILLTMVDVRTRISNQAIEALRGMFGDKVFRVMIRQNTK
                     VIEASARGVPVLKSASSSPPAQAYRQLAREVLSGQEAGL"
BASE COUNT          563 a          869 c          981 g          547 t
ORIGIN      
        1 caaggaggtg cccgccccca cattgtaaca acctggttac cgctcgttaa cacgccagta
       61 acacggcact ggtaagataa aggtgacagc cggcgcacgg cgcttctcct gtgcaagggc
      121 acaccgcaga gggagaccgg cagggtggct cttggggaag gtgatatcgg cggcgggtgg
      181 ggcgactcgc ggagcgcccc aagaaggttg gcattcgctg gcagccagca ccgaggaagg
      241 cgctggctgc cggcaggatc ggttcgtgac aagggtcgct ctgtggggcg gcgccagtag
      301 aggagagcag acattgaaga caccaggcga ggtgattgcg ttcgcgaggg ggaacggcat
      361 ccgggtggtc gacttccgat ttgtggatct gcctgggatc tggcagcatt tctcgatccc
      421 ggtccgccac ctcaccgagg agcttttcga gaatggcatc ggctttgatg gttcatccat
      481 ccgcggcttc aaggagatcc atgagtcgga tatgatcttg atcccggatg cgcggacggc
      541 gttcgtggat gcgttcaccc aggagccgac gctcaacatc ctgtgtgatg tccatgatcc
      601 gatcacgcgg cagccctata cgcgcgatcc gcgctatatc gcgcgccggg cggaggagta
      661 tctaggcagc accgggatcg cggatacggc ctacttcggg ccggaggcgg agttctacat
      721 cttcgatgac gtgcgcttcg acagcacgac gcactctgct ttctacgcgg tcgattcctc
      781 cgaggccgcg tggaacaccg ggcgagacga gcggccgaac cttggctaca agattcgtta
      841 caaggagggc tacttcccgg tgccgcccat ggaccagttc atggacctgc gcaccgagat
      901 gatgctcgag ctggagaagg ccggcatcga gacggagttg catcaccacg aggtggcgac
      961 tgccgggcag gccgagatcg acgtccggta tgatacgctg ctggcgaccg ccgacaagct
     1021 gatgaagttc aagtacgtgg tgaagaacgt ggcgcgccgg aacaaccgca cagtcacctt
     1081 catgccgaag ccgctgttcc aggacaatgg ttcggggatg cacgtgcatc aatcgctgtg
     1141 gaaggatggg aggaacctct tctgggacga gcgcgggtat gccggcctga gtgagatcgc
     1201 catctattac atcggcgggt tgctgaagca cgcgccgagt attctggcgt ttgcggcgcc
     1261 aactacgaac tcgtaccgcc ggctggtgcc cggctacgag gcgccgatca atctgatcta
     1321 cagccagcgg aaccggtcgg cgtgcgtgcg gatcccggtc tacagcaaga gcccgaatgc
     1381 aaagcggatt gagttccgac cgccggatcc ctccagcaac ccctacctgt gctttgcggc
     1441 gctcctcatg gccggcctgg atggtatcca aaaccgcatc atgccgcccg agccgatcga
     1501 taaggatctg tatgatctgc cgccagagga gaaggcggcg gtggagagca cgcccgccag
     1561 cctcgcggat gcgctggatg cgctggaagc cgaccacgac ttcctcctca aaggcgatgt
     1621 ctttacagaa gacgcaattc gggcctggat cgatttcaag cgcaagcgcg aggtggaaca
     1681 ggttgcgctg cgcccgcacc cgcatgagtt tgccctctac tacgacatct gagcgagcgc
     1741 gcgggtgagc cgcgcggtga tgaagaggtg gcgaagggcc atgccagggg cccttcgcca
     1801 cgcccgcgcc ttccgatgag ggtgcacatc ctgcccgcgt gttcgggtct ccgtgcatcc
     1861 acgtccactt tcaaccacgg tagttcccac accacgtggg cgaccgctgt atctctcctt
     1921 tttgccatcc cgctatgccg catttgcctg tggtggagag gctctgcgtt gccagcaggg
     1981 gtgatggtgc ggcgcgtgcc cttgctacgg tgcggagggt tatgctatac tggaggtgcc
     2041 acagtccgct gcggaaatcc tcggatggag ttccacccag cgctccgccg cagcactcgc
     2101 gacagcaagg tgaggccgga tagaccatgg cgaagatcat ttccatcgcg aatcagaagg
     2161 gcggcgtggc aaagaccaca acgaccgtca acctgggggc ggcgctcgcc gaggcgcatc
     2221 agcgcgtgct gctcatcgac ctcgatccac aggcgcacct ggccctgagc ctcggtctca
     2281 accccgatga cctggatgcg agcaccttca acgtgttggc ggagccctca gtcggctttc
     2341 ctcgcatcat caccccgatc tccgagggac tggccctggc gccggcggac atcaacttgt
     2401 cggtggcaga gcagcgcctc ggtgggacaa ttgggcgtga gcagatcctg cgcgataagc
     2461 tgcgcgaggt tcaggccgat tacgactaca ttctgatcga ctgtccgccc agcctggggg
     2521 ttcttacggt aaacgcgctc gcagcctcgc agcgggtgat catcccggtt caggcgcatt
     2581 acctgagcat gaaggggatg caactgttga tcgagacgat cggcctggtg cgggagaagc
     2641 tgaacccgca gctggagatc ctgggaattc tcctgacgat ggtggatgtg cgcacgcgga
     2701 tctcgaatca ggcgatcgag gcgttgcgag ggatgtttgg cgataaagtg ttccgcgtga
     2761 tgatccgcca gaatacgaag gtgatcgagg cttcggcgcg tggcgtgccg gtgctcaagt
     2821 cggcgtcgag ctcgccgcct gctcaggcgt atcgccagct cgcacgggag gtcctgagtg
     2881 gccaagaagc cggtctttga tttcgggttg ggacaggcgc cgcgggtgcg agagcgccag
     2941 gagtggatgg aggtggctca
//
LOCUS       DAOSCV010000565         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21575_length_2959_cov_4.252066, whole genome shotgun sequence.
ACCESSION   DAOSCV010000565 DAOSCV010000000
VERSION     DAOSCV010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21575_length_2959_cov_4.252066"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLY56_13290"
     CDS             <1..939
                     /locus_tag="PLY56_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22500.1"
                     /translation="TGKESPMAIVGPQAVLKLIREISVGEIRKQAERVPRVVICGTEE
                     ERESLRALLGTGTRDEADRARLEQALVEVQCPLEKEEILRSIAAQVLFTVMPPDDTLR
                     RLKVPVYRVSSQESLRRAGEQIAENQEEWLLSLGRHLPGLRPLLAEHLTATTSAANAQ
                     IALISALPGIVPATQILLPTAAAADIVLLTKNQILLVLKLTAIYGKPVNSVARLWELA
                     PVVGGAFGWRALARELVGAVPGGVGVVAKSAIAYAGTYTVGRAAQLYYERGKLPSVEE
                     RKRIYQETLDWAKEVAARMYARLRPNHEKEHGTSEG"
     gene            929..1753
                     /locus_tag="PLY56_13295"
     CDS             929..1753
                     /locus_tag="PLY56_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ22501.1"
                     /translation="MRGEAVNDGVRPRAAGVVDSHAHLNHEDYQPDLDAVVAHAREAG
                     VVAILIPGYDLPSSREAVRLSERLPGVYASVGIHPHDAETCTDAALAELRALLKHPRV
                     VGVGECGLDYYRDLSPRDVQQRVLRAHLALAREANRPIILHNRDSDADLTRILREEGL
                     PEAGGVLHCFSGDAALAAEALGMGLAIGVAGQVTFKNASALREVIASLPLERLMVETD
                     SPYLAPVPYRGRRNEPAHVVTVAECVALLHGTTLDAVANATTARFETLFGVRVVGG"
     gene            1757..2179
                     /locus_tag="PLY56_13300"
     CDS             1757..2179
                     /locus_tag="PLY56_13300"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOJ22502.1"
                     /translation="MEEKQEGFEVVDKRRVNAETETPTEEPPPAAKAASEEAPAGQTD
                     QAAGEGQFPPEVDIYVLLTSMIGMLHGFAWQKMGFIANQKTGQVEEDLPQAKIAVDTI
                     QYLVGHLEGKLSEPEIRELRRVVMDLQMNYLRKSTGGK"
     gene            2169..>2959
                     /locus_tag="PLY56_13305"
     CDS             2169..>2959
                     /locus_tag="PLY56_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOJ22503.1"
                     /translation="MESRKRASGEAKERIAPPDSPIAPGALCRVRVSVADLWSRPVPG
                     SERVSQVLLGTPLRVLAECGDYAYVESPDSYRGWTLQAETVPVASLDKPDHVISDLIV
                     PAYPAPEASEPRLELYYGTRLTAGESAGERRQVLLPDGPAWVPATSLRRIEYEPLAPP
                     SPRRRETTPEGAESDHPLDVEALLADAHRWVGVSYLWGGGTPRGVDCSGFVQVLFGVH
                     GRRLLRDARLQVRHGISVAVTEVQAGDLLFFGRSRWNLPTHVGIAV"
BASE COUNT          491 a          981 c         1015 g          472 t
ORIGIN      
        1 accggcaagg agagtccgat ggcgatagtg gggcctcagg cagtgctgaa gttgattcgt
       61 gagatctccg taggcgagat ccgcaagcag gcggagcgtg tgcctcgcgt ggtgatctgc
      121 ggcaccgagg aggagcggga gtccctgcgc gcgctcctgg gcacgggcac ccgcgatgaa
      181 gccgaccgcg cccgcctgga gcaggcgctc gtcgaagtcc agtgtccttt ggaaaaggag
      241 gagatcctgc gctccatcgc ggcgcaggta ctcttcaccg tgatgccccc agacgataca
      301 ctgcgccgtc tgaaggtgcc cgtgtaccgt gtctccagcc aggagagcct ccggcgcgcc
      361 ggcgagcaga tcgccgagaa ccaggaggag tggttgctca gcctgggccg gcacctgccc
      421 gggttgcgcc cgctactcgc cgagcacctc acggcgacga cctccgccgc gaacgcccaa
      481 atcgcgttga tcagcgcgct tccaggcatc gtacccgcca cccagatcct tctgccaacg
      541 gcggccgccg ctgacatcgt cctgttgacg aagaaccaga ttctcctcgt gctgaagctc
      601 accgccatct acgggaaacc ggtcaacagc gtcgctcgcc tctgggaact cgcccccgta
      661 gttgggggcg cctttggctg gcgtgccctg gcacgcgaac tcgtcggcgc ggtgcccggc
      721 ggcgtcggtg tcgtcgccaa gagcgccatc gcctacgcgg ggacctacac cgttggacga
      781 gccgcccagc tctactacga gcgcggcaag ctgcccagcg tcgaggagcg gaagcgcatc
      841 tatcaggaga ccctcgattg ggctaaggag gtcgccgcgc ggatgtatgc gcgcctgcgc
      901 cccaaccacg agaaggaaca cggaacaagt gaggggtgaa gcagtgaacg atggcgtccg
      961 gcctcgggcg gccggcgtcg tggacagcca cgcccacctg aaccacgagg actaccagcc
     1021 ggacctggat gccgtcgttg cgcatgcccg cgaggcaggg gtcgtcgcga tcctcattcc
     1081 cggctatgac ctcccatcga gccgggaggc cgtgcgtctc tcggagcggc tcccgggcgt
     1141 gtatgcctcg gtagggatcc acccgcacga tgccgaaact tgcactgacg cagcgttggc
     1201 cgaactgcgc gcccttctga agcatccgcg cgtggtggga gtcggcgaat gcggtctcga
     1261 ttactaccgc gacctctccc cgcgcgatgt gcaacagcgg gttctgcgag cgcacctggc
     1321 gctggcccgc gaggcaaatc gcccgatcat cctgcacaac cgcgactcgg atgccgacct
     1381 cacacgcatc ctgcgggagg agggccttcc ggaagcggga ggcgtgttgc attgcttctc
     1441 gggcgacgcg gcgctggcag cggaagccct cgggatgggc ctggccatcg gggtggcggg
     1501 gcaggtgacc ttcaagaatg cctccgctct gcgggaggtg atcgccagct tgcccctgga
     1561 acgcctgatg gtggagacag actcgcccta cctggcgccg gtgccgtatc gcggccggcg
     1621 caacgagccg gcgcatgtcg tcaccgtggc ggagtgtgtc gccctcctcc acggcaccac
     1681 acttgatgcc gtggccaacg ccaccacggc gcgtttcgag accctattcg gagtccgtgt
     1741 agtgggaggt tgagtgatgg aagagaagca agaggggttc gaggtcgtcg acaagcggcg
     1801 ggtgaacgcc gagacagaga cgccgaccga ggagccaccg cccgctgcca aagccgcgag
     1861 cgaggaggcg cccgccgggc agacggatca ggctgccgga gagggccagt tcccaccgga
     1921 ggtcgatatc tacgtgctcc tcacctcgat gatcggcatg ctgcacggat tcgcctggca
     1981 gaagatgggc ttcatcgcca atcagaagac gggccaggtg gaagaggacc tgccgcaggc
     2041 gaagatcgcg gtggatacca ttcaatacct ggtcgggcat ctggagggga agctctcgga
     2101 gcccgagatc cgggaactgc ggcgcgtggt gatggacttg cagatgaact acctcagaaa
     2161 gagcaccggt ggaaagtagg aagcgtgctt ccggcgaggc gaaggagcgg atcgccccgc
     2221 cggattcccc catcgccccc ggcgcgctgt gccgtgtgcg ggtttccgtg gccgatctgt
     2281 ggtcgcgtcc tgtccccggt tccgagcgcg tgagccaggt gctactcgga acgccgcttc
     2341 gggtcctggc ggagtgcggt gactacgcct atgtcgagag ccccgactcc taccgtggct
     2401 ggacgttgca ggcagagacc gtgcccgtcg cctcgctgga caagccggac cacgtcatca
     2461 gcgatctcat cgtgcccgct taccctgccc cggaagccag cgagccgcgc ctggagctct
     2521 attatggcac gcgcctcacc gccggcgagt ccgccggcga gcgccggcag gtgctcctgc
     2581 cggatggccc ggcctgggtc ccggccacat cgctgagaag gatcgagtac gagccgcttg
     2641 cgcccccctc tccccgtcga agggagacaa cgcccgaggg ggccgagtct gaccatccgc
     2701 tggacgtgga ggccctcctc gcggacgcgc atcgctgggt gggcgtgtcc tacctctggg
     2761 gcggcgggac cccgcgaggt gtggactgct ccgggtttgt ccaggtgctc tttggcgtcc
     2821 atggccgacg cctgctccgc gacgcccggc ttcaggtgcg ccacggtata tcggtcgccg
     2881 taaccgaggt gcaggcgggc gatctcctct tcttcggccg gtcgcgctgg aacctgccga
     2941 cgcacgtcgg tatcgccgt
//
LOCUS       DAOSCV010000566         2958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21586_length_2958_cov_3.707888, whole genome shotgun sequence.
ACCESSION   DAOSCV010000566 DAOSCV010000000
VERSION     DAOSCV010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21586_length_2958_cov_3.707888"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..555)
                     /locus_tag="PLY56_13310"
     CDS             complement(157..555)
                     /locus_tag="PLY56_13310"
                     /inference="COORDINATES: protein motif:HMM:NF021237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOJ22504.1"
                     /translation="MITREQQRMRLAWDRLCYRFGDDDASWRNDGRAAKYLGALKKTP
                     ARIHACGLGMALAFLRSRSSQREAQRAGEDVEVATLRALGYQNDTDLIEKIRNRDAAF
                     LFLATDEAVALIAWMTRLMEGAGVSADEEN"
     gene            complement(573..1553)
                     /gene="cmr4"
                     /locus_tag="PLY56_13315"
     CDS             complement(573..1553)
                     /gene="cmr4"
                     /locus_tag="PLY56_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOJ22505.1"
                     /translation="MSSGILFLRAQTPMHPGSGTSVGAVDLPVQRERYTNWPVIQGSA
                     LKGVLRSVHRDYLVGAQHQAGNTSYTLEDADADPELVEVFGKAGEEGELHAGALAVTD
                     ARILAFPVRSAKGIFVWVTCAAVLSRAIRDLQMAGLTSPFDLPAAPGTGKAVSTSNAI
                     DIWVEMENGPKLVLEDILFTPVTAGREELDRFAGWLATAGCGYAEEIANGKTEDGDPR
                     RRLVLISGDAFKHLVTYATEIATRIRLGLETKTVVGGALFTQELLPAETIFYSVLLAS
                     KPRSQNPMLDAAGVIGKIKSVVEEAKVLQVGGDETTGKGWCWARVWPQAV"
     gene            complement(1546..2766)
                     /gene="cmr3"
                     /locus_tag="PLY56_13320"
     CDS             complement(1546..2766)
                     /gene="cmr3"
                     /locus_tag="PLY56_13320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr3"
                     /protein_id="HOJ22506.1"
                     /translation="MRRLNFILTPADVTVFRDARPFEPGATSSRSHFPPPRAVAGAIR
                     TWLLSGLGTDFAKFSNLRRKRRGSESEYQAMLDDLRACTPDEAAWILDAHLAGPLLCE
                     ANTGSTIYPAPFHLARFVDTTDKDGLVRIFPWKRGDPAKLIGLEPPEGAPSPNLWRPG
                     LVRDPREWKPIADKFVSQEDLVKVLTVQEEMSDVKLFEPGMFYTHEPRLGIGMDPAKR
                     SNKEGLLYTTEFLRFGWADQDGERPYGLRVDLEIPDSAGEDAAEKVKELADKIRWLPI
                     GGEGRVTAIRVDDDKGFPEGPKWPPENGRFLTYLATPGLFSEGRWYPTELTKTCDLVS
                     AVTGAPRAVSGWDVAAGRPLKTRFVIPAGAVYFWQIRGDTLPEDPHGKSICDSLEDRQ
                     AGWGLVLRGEWNYV"
     gene            complement(2769..>2958)
                     /locus_tag="PLY56_13325"
     CDS             complement(2769..>2958)
                     /locus_tag="PLY56_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22507.1"
                     /translation="RGEGTPQGVIDQVMTLWNETWAFLNDPNRPGPEQDDEQFGNPVF
                     NAFLNHVGIASFLVRGGE"
BASE COUNT          551 a          974 c          879 g          554 t
ORIGIN      
        1 gagccttttg gtcaaaactg ttgtgactcg ggaacgcaac gtagcggtcg agcaggaggc
       61 cggcgtgcgc tacttgatct ccaccctcgg cccactcgtg gaggccgcgc atcaatggat
      121 agaacgccat ctctctcctc caacaagacg ccgcgctcaa ttctcttcgt cggcgctgac
      181 gcccgcaccc tccatcaggc gggtcatcca ggcgatcaga gccaccgcct catcggtggc
      241 caggaaaagg aaggcagcat cgcgattgcg gatcttctca atcaagtcgg tgtcgttctg
      301 atagcccagt gcccgaaggg tagcaacttc cacgtcctcg ccagccctct gcgcttcccg
      361 ctgggaagag cgcgagcgca agaacgccag ggccattccc aacccgcagg cgtggatccg
      421 cgccggagtc ttcttcagcg ctccgaggta cttggccgcg cgcccgtcat tgcgccagga
      481 agcatcatcg tctccgaatc gatagcacaa acggtcccag gccaggcgca ttcgctgttg
      541 ttctctggta atcatagttt acctcccgct gtctacacag cctggggcca cacacgtgcc
      601 cagcaccatc ccttgccggt cgtttcatcg ccgcccacct gcagcacctt cgcttcttcc
      661 accacgctct ttattttgcc aatgacaccg gccgcatcga gcattggatt ctgtgaacgc
      721 ggcttgcttg cgaggagcac gctgtagaag atcgtctcag ccggcagcaa ctcctgcgtg
      781 aagagcgccc cgccgacgac agttttggtc tcaagaccga gccggatccg agtggcgatc
      841 tcggtagcat aagttaccag gtgcttgaag gcgtcgccgc tgatcaacac tagccgcctc
      901 cgggggtcgc cgtcctctgt tttgccgttt gcgatctcct cggcataacc gcagccggcg
      961 gtggcgagcc atcccgcaaa ccggtccaac tcctcccggc cagcggttac cggtgtgaac
     1021 aagatatctt ccagcacgag ctttggcccg ttctccatct ccacccagat atcgatggcg
     1081 ttgctggtag agacagcctt acccgtaccg ggcgcggcgg gcaggtcgaa cggagaggtg
     1141 agtccggcca tctgcaggtc gcggatggcg cgggagagga cggcggcgca ggtcacccag
     1201 acgaagatgc ccttggccga gcgcaccggg aaggcgagga tacgggcgtc ggtaacggcc
     1261 agggctccgg catgcagctc gccttcctcg ccagctttgc cgaacacctc cacgagttcc
     1321 ggatctgcat cagcgtcctc gagtgtgtag ctcgtgttcc ctgcctggtg ctgggctcct
     1381 accaggtaat cgcgatgcac gctgcgcagg acacccttga gcgccgagcc ctgaatcacc
     1441 ggccagttcg tatagcgttc ccgctgcacc ggcaggtcca cagcaccgac tgaggtgcct
     1501 gacccggggt gcatcggggt ctgcgcacgc aggaagagga tgccactaga catagttcca
     1561 ctctcctctc agaaccaggc cccacccggc ctgccggtcc tccaggctgt cgcagatgct
     1621 cttaccatgg ggatcctccg ggagggtatc tcctcgtatc tgccagaaat agaccgcccc
     1681 ggccgggatg acaaaccgcg tcttcaacgg gcggcccgcc gcaacatccc agccggacac
     1741 agcgcgcggg gcaccggtga cggcggaaac caaatcgcac gtcttggtca attcggtagg
     1801 ataccaccgg ccttctgaga acaggcccgg ggtcgccagg taggtgagga agcgcccatt
     1861 ctccggaggc cattttggcc cttcaggaaa gcccttatca tcatccacgc ggatagcagt
     1921 cacgcggccc tcgcccccga ttggcagcca gcgtatctta tccgccagct cctttacctt
     1981 ttccgcagca tcttctcccg ctgaatcggg aatctccagg tcgacccgaa gcccgtaggg
     2041 ccgctctcca tcctggtcag cccagccaaa gcggagaaac tccgtggtgt agagcagacc
     2101 ttccttgttc gagcgcttcg ccggatccat cccgatgccg aggcggggtt catgagtgta
     2161 gaacatcccc ggctcgaaga gcttgacgtc ggacatttcc tcctgaacag tgagaacctt
     2221 cacgagatct tcttgggaga cgaatttatc ggcgatcggt ttccattcgc gagggtccct
     2281 aacgaggccg ggccgccaca aattgggaga aggcgcaccc tcgggcggct ccaggccgat
     2341 cagcttcgcg gggtcgcccc gcttccacgg gaagatacga acgaggccgt ctttgtccgt
     2401 ggtatcgaca aacctggcga gatgaaacgg agccgggtag atggtagaac ccgtattggc
     2461 ctcgcacagc agcgggcccg ccaggtgggc gtcgagaatc cacgcggctt cgtctggcgt
     2521 gcaggcacgc aagtcatcga gcatcgcctg atactcagac tcgctgccgc gccgctttcg
     2581 cctgaggttg ctgaacttgg cgaagtccgt gccgaggcca ctcaacagcc aggttcggat
     2641 ggcgccggcc acggctcgcg gcggcgggaa gtggctgcgc gagctggtcg cgcccggctc
     2701 gaaaggccgg gcatcgcgga agacggtgac atccgctggt gtgagaatga agttgagacg
     2761 ccgcatgatc actcccctcc gcgtaccagg aacgaagcga tccccacgtg gttgaggaac
     2821 gcgttgaaca ctgggttacc gaactgctcg tcatcctgct cgggccccgg gcgattcggg
     2881 tcgttgagaa acgcccaggt ctcgttccac agggtcatca cttggtcgat aaccccctgg
     2941 ggtgtgcctt cgccgcgc
//
LOCUS       DAOSCV010000567         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21595_length_2957_cov_3.464163, whole genome shotgun sequence.
ACCESSION   DAOSCV010000567 DAOSCV010000000
VERSION     DAOSCV010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21595_length_2957_cov_3.464163"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>537
                     /locus_tag="PLY56_13330"
     CDS             4..>537
                     /locus_tag="PLY56_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ22508.1"
                     /translation="MGNPFQNLQTQRETASVTAIESFYELKYRLLDRLIAQLDQEFLA
                     RGDEMALRRQVQEIILNLLDEQGVAVSRTQRNQLVFELLDEVTGLGPLQALLNDPSVS
                     EIMVNGPKQIWYERDGRLQLSEKQFRDDEHLIGIIQKICSAVGRRIDEASPLVDARLQ
                     DGSRVNAVIPPIALRGAS"
     assembly_gap    538..547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <548..1334
                     /locus_tag="PLY56_13335"
     CDS             <548..1334
                     /locus_tag="PLY56_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOJ22509.1"
                     /translation="TIRKFSRDALTIEDLISFGTLTHGMADFLRAAVLAKLNIVVSGG
                     TGSGKTTLLNCLSGFIPADERIVTIEDAAELQLKQPHVVTLEARPPNIEGKGAVTIRD
                     LVVNSLRMRPDRIVVGECRRGEALDMLQAMNTGHDGSLTTGHANSPREMLSRLETMVL
                     MSGMELPVRAIREQIAAAIDLIIQQARLRDGSRKIIQITEVVGMEGDVITLQDIFVLR
                     QLGVDKNNKVIARHEATGLRPKCLEKFEQAGISLPASLFAPGR"
     gene            1345..2340
                     /locus_tag="PLY56_13340"
     CDS             1345..2340
                     /locus_tag="PLY56_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOJ22510.1"
                     /translation="MNSRLLIYGILFGIAGAYLSIRGLMQIVPIASILLHRRSKRILQ
                     ERLGKKSQKDAPESDASAPQKPRTLLPMLTRILSRTRIRPILAQLDHALALARIPLKA
                     VEFLYITVTALFLTLAIVQFFSSNLFVALTVALVAGFAPFYVLRISQRMRFAKIDLQV
                     SDALLLMTNSLRSGTSFLDAMEVVSREMPPPISEEFERTLRDITLGVPVQEAFLKMTQ
                     RCHSEDLDLAVTAFMIQREVGGNLAEILENIAETIRMRVKLKREIQTLTAQGKLSGGI
                     LCALPIGLTLLLTSVNPDYMNLLLTTAMGKGMLIAGGVLQVIGILAIMRVVKIEV"
     gene            2349..>2957
                     /locus_tag="PLY56_13345"
     CDS             2349..>2957
                     /locus_tag="PLY56_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22511.1"
                     /translation="MLVLIALTFFLSIIFLTIGIRIERESTSPLARLQRQTKETQELT
                     PEEAELRRPVLARLIGPVAQRALLLVARFSPPGLKDSIRRRLQIAGNPWRAHSEEVIF
                     AKIVLAATGAIVGLALGAAVLHKKPEQSVLFALGGSAVGFLLVDWMILQKTKQRRKQI
                     QHSLPYVLDLLCVSVEAGLAFDAAMQKVTEKVEGPLSQEFQQV"
BASE COUNT          557 a          942 c          900 g          548 t
ORIGIN      
        1 gcgctgggca accccttcca gaacctgcaa acccaacgcg agacagcctc cgtcaccgcc
       61 atcgagtcgt tctacgagct aaaataccgc cttctcgacc gcctgatcgc acaactcgat
      121 caggagtttc tagcgcgcgg cgacgagatg gccctccgtc gccaggtgca ggagatcatc
      181 ctcaacctgc tggacgaaca gggcgtcgcc gtcagccgca cgcagcgcaa ccagcttgtc
      241 ttcgagctcc tggacgaggt gacgggtctc ggaccacttc aggccctcct gaacgacccg
      301 tccgtgagcg agatcatggt gaatggcccg aagcagatct ggtacgagcg cgacgggcgg
      361 ttgcagcttt cggagaagca attccgcgac gacgagcacc tgatcggcat catccagaag
      421 atctgttcgg cggtgggccg gcgcatcgat gaggcaagcc cgctcgtgga tgcgcgtctc
      481 caggatggct cgcgcgtgaa tgccgtcatc ccacccatcg ccctgcgcgg ggcatcgnnn
      541 nnnnnnncac gatccgcaag ttctcgcgcg acgcgctcac catcgaagac ttgatctcct
      601 tcggcaccct gacccatggc atggccgatt ttctgcgcgc cgcagtgctc gccaagctga
      661 acatcgtggt ctccggaggc acgggatcgg ggaagacgac gctcctcaac tgtctctccg
      721 ggttcatccc ggcggatgag cggatcgtca caatcgagga tgccgcagag ctgcagctca
      781 agcagcctca cgtcgtcacc ctggaggcgc gcccgccgaa catcgagggc aaaggtgcgg
      841 tcaccatccg ggacctggta gtgaactcgc tccgcatgcg ccctgaccgg atcgtcgtcg
      901 gcgagtgccg acgcggcgag gccctcgata tgcttcaggc gatgaacacc ggccacgacg
      961 gctcattgac gactggccac gccaactcgc ctcgcgagat gctctcgcgc ctggaaacga
     1021 tggtcctgat gtcgggcatg gagctgccgg tgcgcgccat tcgcgagcag atcgccgcgg
     1081 cgattgacct catcatccag caggcgcgat tgcgcgatgg ctcgcgcaag attatccaga
     1141 tcaccgaagt cgtcgggatg gagggcgatg tgatcacgct gcaggatatc ttcgtcctgc
     1201 gccaactcgg cgtcgacaag aacaacaagg tgatcgcccg gcatgaggca accggattgc
     1261 gcccgaaatg cctggagaag tttgaacagg cgggaatcag ccttcccgct tcgctctttg
     1321 cgccagggcg ttgaggaggt ttcggtgaac agccggctgc ttatctatgg catcttgttc
     1381 gggatcgcgg gcgcgtacct ctccatcagg ggcctgatgc agatcgtccc aatcgcaagc
     1441 atcctgctgc accggcgatc gaagcggatc ctgcaagagc ggctgggcaa aaagagccag
     1501 aaagatgccc ccgagtcaga cgcctctgcc ccgcaaaaac cgcgcaccct gcttccgatg
     1561 ctcacgcgca tcctctctcg gacgcggatt cggcccatcc tcgctcaatt ggaccatgca
     1621 ttggccctgg cgcgcattcc actgaaggcg gtagagtttc tctacatcac cgtcaccgcg
     1681 ctcttcctga cgctggcgat cgtacagttc ttttcgagca acctctttgt ggcgttgacg
     1741 gtcgccctag tggccggctt cgcgcctttt tacgtcttgc ggatcagcca acggatgcgg
     1801 ttcgcgaaga tcgatctcca ggtttcggat gccctgctcc tgatgacgaa ctcgttgcgc
     1861 tcgggaacga gcttcctgga tgcgatggag gtggtgtcgc gcgagatgcc tccaccgatc
     1921 agcgaggagt tcgagcgcac gctacgtgat atcacactcg gcgtgccggt ccaggaagcc
     1981 ttcctcaaga tgactcagcg ctgtcacagc gaggacctgg acctcgcggt cactgcgttc
     2041 atgatccaga gggaggtggg cggcaacctg gcggagatct tggaaaacat cgccgagacg
     2101 atccggatgc gcgtgaagct gaagcgtgag atccagacat tgacggctca gggaaagcta
     2161 tccgggggca tcctctgcgc gctcccgatc gggctgacac tgttactcac atccgtgaac
     2221 ccagactata tgaatctgct cttaaccacc gcgatgggca agggaatgct gatcgcgggc
     2281 ggcgtgctgc aagtgatagg gattctggcc atcatgcgcg tggtcaagat cgaggtatag
     2341 caggaacgat gctggttctc atcgctctca ccttcttcct gtcgatcatc ttcttgacga
     2401 tcggaatccg gatcgagcgg gagagtactt cccctctggc gcggctgcag cgccagacga
     2461 aggagactca ggagctgacc ccggaagagg cggaactgcg ccggccggtc cttgcgcgcc
     2521 tcatcggccc cgtcgcgcag cgcgccctcc tgctggtggc ccgcttctca ccacccggcc
     2581 tcaaggacag catccgacgc cgcctgcaga tcgcgggcaa tccctggcgc gcccactcag
     2641 aggaggtaat cttcgccaag atcgtgctcg ccgccaccgg cgcgatcgta ggcctcgcgc
     2701 tgggcgccgc ggtgctgcat aagaagcccg agcagtccgt gctgttcgca ctgggcggct
     2761 ccgccgtcgg ctttctcctg gtcgattgga tgatcctgca gaagacgaag cagcggcgca
     2821 agcagatcca gcactctctg ccctacgtcc tcgacctcct ctgcgtgagc gtcgaggccg
     2881 ggctggcgtt cgatgcggcg atgcagaagg tgaccgagaa ggtggaaggg cccctctcgc
     2941 aggagtttca gcaagtg
//
LOCUS       DAOSCV010000568         2952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21635_length_2952_cov_3.325854, whole genome shotgun sequence.
ACCESSION   DAOSCV010000568 DAOSCV010000000
VERSION     DAOSCV010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21635_length_2952_cov_3.325854"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            338..1564
                     /locus_tag="PLY56_13350"
     CDS             338..1564
                     /locus_tag="PLY56_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HOJ22512.1"
                     /translation="MHRIGDARFVLLGEATHGTSEFYSWRARLTKRLLGEKGFSFVAV
                     EGDWPHCYQVNRYVKGDPAAGRTASQVLRMFDRWPTWIWANQEMVSFVEWLRRYNRQV
                     PRGMEAGFYGLDTYSLWESLRAVVEYLAQVDPAAAEVARQAHHCFQPYGDVRDYSMAT
                     RLVPESCEDEVVALLKEILMRRPEIPGQDPEALFNAEQNAWVVRDAERYYRAFISAGP
                     EAWNIRDRHMADTLDRLMEFHGPGAKAIIWAHNSHIGDARASEMADRGMVNLGQLVRE
                     RHVDQGVISVGFSTHRGSVVAADAWEAPMEFMVMPPAREGSWEDVIHRAGAEDKLLLL
                     DEGTPPEAMRAQRGHRAVGVVYDPKYEGFTNYVPSVLPERYDAFLYFDETEALHPLHL
                     PARAAPAMPETYPAGV"
     gene            complement(1578..>2952)
                     /locus_tag="PLY56_13355"
     CDS             complement(1578..>2952)
                     /locus_tag="PLY56_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22513.1"
                     /translation="VRLDSRPSPVLLKAPLARATETAQRLANSVKGTPDEAQGTRMVQ
                     SFAAFAREIDRLESVNTARYQELAARLNLLQEEQAGLRRKVLVARGGIRGASTYAEFL
                     TFPQRKANLHCHTRHSDGSLPCDEMAAWYAENGYQIVALSDHDAYGDQDGGVLSSFFQ
                     KDQDVHDWDGDGVTHPTREYRSGAEAYVRDYDKPAPPWVPRNWKLNRPGEFVVLNSME
                     SSFGHHHTNVIEPPPGKIPRPREGYGFVDWCHANNGIVFVVHPGDWNKAPERLLDHPE
                     MRKIDGLEVMNGFTARENRKGDNPDGSKGIAEQLWDACLDAGKLLWGFANDDAHNTKP
                     DHYAGAGSAWNMVWVRELTKPAVMESLRRGAFYASCGIQIDQVEMDAESITVRSPNAT
                     HIKVVGDGGRTLAQVDANQITYRLQGTEKWIRVVLWNDTICYPNEPSRYPQRAWLQPI
                     VLDSLIP"
BASE COUNT          499 a          909 c          997 g          547 t
ORIGIN      
        1 ccgccctatc cgatccatag tgaaggcgcc aacttcgcct tttgcgatgg ccatgtgaag
       61 tggatccaca acagcaagta cgccgattgg agcggcgccg acaccacgac gccggatgga
      121 atgcccgatc ccgcactgtg gaagctacaa tagagcaatc gccgacgcgg agggaggttg
      181 gcctgggttc tcatggggta tgagggcgtg acacttcgtt gcgaaggagc cacgacccat
      241 gagaaccatt gagacgacgg ccggtacgga ggagctggta acgcacatcc gcgatatcgc
      301 gcgtcctctg cgggacgacg aggatctcga tcctctgatg caccggatcg gcgatgcgcg
      361 ctttgtgctc ctgggcgagg cgacgcatgg gacatcggag ttctactcct ggcgcgcccg
      421 gctgacgaag cgcctgcttg gggagaaggg gttctccttt gtcgccgtcg agggcgactg
      481 gccccattgc taccaggtga accgctatgt caagggagat ccggcagcgg gtcgcaccgc
      541 ctctcaggtg cttcgcatgt tcgaccgctg gccgacgtgg atatgggcca accaggagat
      601 ggtttcgttt gtcgagtggc tgcgccggta taaccggcag gtgccacgcg gcatggaggc
      661 gggcttctac gggctggata cctacagcct ctgggagtca ttgcgcgccg tagtggagta
      721 tctggcacag gtggatcccg cggccgccga agtggcccgg caggcgcacc actgttttca
      781 gccgtatggc gacgtgcggg attactcgat ggcgacccgg ctcgtgcctg aatcgtgtga
      841 ggatgaggtg gtggcgctcc tcaaggagat cctcatgcgc cggcccgaga tcccagggca
      901 ggatcccgag gcgctcttca atgccgagca aaacgcctgg gtggtgcgcg atgcagaacg
      961 ctactaccgc gctttcatct cggcggggcc ggaggcatgg aacatccgcg acaggcacat
     1021 ggcggatacc ctcgaccggt tgatggagtt ccacggcccg ggcgcgaagg cgatcatctg
     1081 ggcgcacaac tcccacatcg gggatgcgcg cgcctcggag atggccgacc gggggatggt
     1141 gaacctcggc cagctcgttc gcgagcgcca tgtggaccag ggcgtgatct cggttggctt
     1201 tagcacgcac cgtggcagcg tcgtcgccgc cgatgcctgg gaagcgccga tggagttcat
     1261 ggtgatgccg cccgcccgtg aggggagctg ggaggacgtg atccaccgcg ctggggcgga
     1321 ggacaagttg ctcctgctgg acgagggcac cccgccggag gcaatgcggg cgcagcgcgg
     1381 ccaccgagcc gtgggcgtgg tgtatgaccc gaagtacgaa ggcttcacca actacgtgcc
     1441 tagcgtgctt ccggagcggt atgatgcgtt cctctacttt gatgagacag aggcgctcca
     1501 cccgctccat cttcccgcgc gcgcggcgcc cgcgatgcct gagacctacc cggcgggtgt
     1561 gtgaggggaa cctccggcta tgggatgagc gagtctagca caatcggctg tagccaggcc
     1621 ctctgcgggt agcgagaggg ctcgttggga tagcagatgg tgtcgttcca gagcaccacg
     1681 cggatccact tctcggtgcc ctgaagccgg taggtgatct ggttcgcgtc gacctgggca
     1741 agagtccgcc cgccgtcacc caccaccttg atgtgggtgg cgttcgggct gcgtaccgtg
     1801 atgctctcgg catccatctc gacctggtcg atttggatgc cgcacgacgc atagaaggcg
     1861 ccccggcgca gcgattccat caccgccggc ttggtgagct ctcgcaccca caccatgttc
     1921 cacgcgctcc cggccccggc gtagtgatcc ggcttcgtgt tgtgggcgtc atcgttggca
     1981 aacccccaga gaagtttgcc cgcatcgaga caggcatccc acagctgctc cgcgatcccc
     2041 ttgctgccat cggggttgtc tcccttgcgg ttttcgcgtg ccgtgaaacc gttcatcact
     2101 tccaggccgt cgatcttccg catctccggg tggtccagga gccgctctgg agccttgttc
     2161 caatcgccgg ggtgaacgac aaagacgatg ccgttgttgg cgtggcacca gtcaacgaac
     2221 ccgtagccct cgcgagggcg ggggatcttg ccgggagggg gctcgatcac gttcgtgtgg
     2281 tggtggccga acgacgactc catcgagttg aggaccacga actcgccagg ccggttgagc
     2341 ttccagttgc gtggcaccca aggcggggcc ggcttatcgt aatcgcgcac gtaggcttct
     2401 gccccagagc gatactcacg cgtggggtgg gtgacgccat cgccatccca gtcgtggaca
     2461 tcctggtctt tctggaagaa ggatgagagg acgcccccat cctgatcccc gtaggcgtcg
     2521 tggtccgaga gcgccacgat ctggtagccg ttctccgcat accaggcagc catctcgtcg
     2581 caggggagag aaccgtcgga gtgccgggtg tgacagtgca ggtttgcctt tcgctggggg
     2641 aatgtgagga actccgcgta ggtggaggcg ccgcggatgc ccccccgagc caccagcact
     2701 ttccggcgca gcccggcctg ctcctcctga agaaggttca ggcgtgctgc cagctcctga
     2761 tagcgggcgg tgttcacact ctcaaggcgg tctatctccc gggcaaacgc tgcgaacgat
     2821 tgaaccatcc gcgtgccctg cgcctcgtcc ggcgttccct tcacggagtt tgccaggcgc
     2881 tgcgccgttt ccgttgcgcg cgcgagaggg gctttcagga ggaccggcga cgggcgcgaa
     2941 tcgaggcgca ca
//
LOCUS       DAOSCV010000569         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21644_length_2951_cov_4.594613, whole genome shotgun sequence.
ACCESSION   DAOSCV010000569 DAOSCV010000000
VERSION     DAOSCV010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21644_length_2951_cov_4.594613"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="PLY56_13360"
     CDS             <1..1260
                     /locus_tag="PLY56_13360"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ22514.1"
                     /translation="WQSYLVKDPVTGQMMWNNQPGDYDGYNERFGGLPAFREAVETYR
                     KMGALVTLYTDPIRCDYNSKAGKEFGERWGVVKPDGKHVDSYDVWNMCHDVAEYREWV
                     AKTMGRVMRETGADGIRLDEYGHRGWACYSTLHEHTYAEPGITQWQKGIAETCRLVRE
                     AMDEVKPDSVLTTEYPGYDYELQFLEGCITYDLTVMATPLRPLECNLQRFYFPECKAY
                     ELDHRAADLKSRKKLWNAVESFGRYYPPAMYALLNENEDVYQGRECEPLVPTLTQYVY
                     ANRFGAGRKVLYHLYNAKGHTYEGPALAVQLGRGEHLFDLLRCREVPVAKGVASIYLE
                     RDDVTVLAKLPRRLNVARQGEMLRVTAQGAPARAQVVISDLEGKVLLSQPATQATLDL
                     SQLPADAKPACVKLLGPNGLLDAVALP"
     gene            1384..2511
                     /locus_tag="PLY56_13365"
     CDS             1384..2511
                     /locus_tag="PLY56_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22515.1"
                     /translation="MSTGAHEIESICEAILAYLVRCQDLDPGEYYGSFWSEKAYHGPL
                     LDYHAGGAHHHRGAGSAGLAHWLVGKQRGDAGLMHRAEWAFDWLVARQRPSGGYLEIQ
                     NNEKPSDWEYTGLEECSTIETAFVIHGLGHALLHGLPPKKSYMECLQRAGLWQLSIEW
                     PAGSGVFPHHERSPYDCLNANLHAAETLMVSFAALRQVYGRPLNIFFQGARRSVLHTL
                     DQQWPNGCFPYRAGEGITINYTSLVLWCLMNTLDLVPEPLRDRFVPRERVDEAVERAT
                     AFLRGCVAEDGSLLWEGNETSTAKHNIWCYALTYHVLRRAGGEENEQAAARLGRFLAS
                     RRTASGLLPMRDQGEEITECAFMQADMLLFLLPFSALASTA"
     gene            2578..>2951
                     /locus_tag="PLY56_13370"
     CDS             2578..>2951
                     /locus_tag="PLY56_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ22516.1"
                     /translation="MLGTPLKGSATRLLLLGSGELGKEVAIEAQRLGVEVIAVDRYPN
                     APAMQVAHRSHVINMLDRSELARVIRQERPDYIVPEIEAIDTRYLLEVEQEGFTVIPT
                     ARATNLTMNREGIRRLAAEELGL"
BASE COUNT          532 a          921 c          999 g          499 t
ORIGIN      
        1 tggcagtcct acctcgtcaa agaccccgtc accgggcaga tgatgtggaa caaccagccg
       61 ggcgactatg acggctacaa cgagcgcttc ggcggcctgc ccgccttccg cgaggcggtg
      121 gagacctacc ggaagatggg cgcgcttgtc acgctctaca ccgatccgat ccgctgcgat
      181 tacaactcca aggcgggcaa ggagttcggc gagcgttggg gcgtcgtgaa gccggatggc
      241 aagcacgtgg acagctatga tgtctggaac atgtgccatg acgtggcaga gtaccgcgag
      301 tgggtggcca agacgatggg ccgcgtgatg cgggagaccg gagccgatgg gattcgcctc
      361 gatgagtatg ggcaccgcgg ctgggcctgc tatagcacgc tgcacgagca cacctacgcc
      421 gagccgggga tcacccagtg gcagaagggg atcgccgaga cctgtcgcct ggtccgcgaa
      481 gcgatggatg aagtgaagcc ggactccgtc ctcaccaccg agtatccggg ctacgactac
      541 gagcttcagt tcctcgaggg gtgcatcacc tatgacctga cggtgatggc aacgccgttg
      601 cgccctctgg agtgcaacct gcagcgcttc tacttcccgg agtgcaaggc gtatgagcta
      661 gaccaccgcg ccgccgacct caagagccgg aagaagctgt ggaacgccgt ggagtcgttc
      721 ggccgctact atccgcccgc gatgtatgct ctcctgaacg agaatgagga cgtctaccag
      781 ggccgagagt gcgagccgct ggtgcccacg ctgacgcagt acgtctatgc caaccgcttc
      841 ggcgcgggac ggaaggtgct atatcacctc tacaatgcga agggacacac ctacgagggt
      901 ccggcgctgg cggtccaact cgggcgcggc gagcacctct tcgatctact gcgctgccgt
      961 gaggtgccgg ttgcgaaggg cgtcgccagc atctatctgg agcgcgacga tgtcacggtt
     1021 ctcgcgaagc tgccccgccg gctgaacgtt gcccgtcagg gagagatgct gcgcgtgacc
     1081 gcgcagggtg cgccggcgcg cgcgcaggtg gtgatcagcg acctggaggg aaaggtgctc
     1141 ctctcccagc cggccacgca agcgacgctg gatctgtcgc agctgcccgc cgatgcaaag
     1201 ccggcatgcg tgaagctgct tggacccaat gggctgctag atgcagtcgc tctcccgtaa
     1261 accgcgcttg cggtgaagag gggtgagctt ctgctgagga atccgatggg ccggggcggg
     1321 atcgccccgg cccgcgaagt tgggcgcggg tgcgcgggat ccggccgcct ggaaaagaga
     1381 ccgatgagta cgggtgcaca cgagatagag agcatttgcg aggcgatcct cgcctacctg
     1441 gtacgctgtc aggacctcga tccgggggag tactacggct ccttctggag cgagaaggcg
     1501 tatcacggcc ctctcctcga ctatcatgcc ggaggggcgc atcaccaccg gggtgcgggc
     1561 agtgccgggc ttgcccactg gctggtgggc aagcagcgtg gcgacgccgg gctgatgcac
     1621 cgcgcggagt gggccttcga ttggctggtg gcccggcagc gcccgagtgg cggctacctg
     1681 gagattcaga acaacgagaa gccctccgac tgggagtata ccggcctgga ggagtgctcc
     1741 acgattgaga cggcgtttgt catccacggt ctgggccatg ccctgctgca tggcctgcct
     1801 cccaagaagt cttacatgga gtgcctccag cgcgccgggc tgtggcagct ctctattgag
     1861 tggccggccg gctctggagt cttcccacac cacgaacgct cgccctacga ctgcctcaac
     1921 gccaacctgc acgccgccga gacgctgatg gtcagctttg ccgccctgcg ccaggtctac
     1981 gggcgtcccc tcaacatctt cttccagggg gcgcggagga gcgtgctgca cacgctcgac
     2041 cagcagtggc cgaacggctg ctttccctac cgcgcaggcg aggggatcac catcaactac
     2101 acctcgctcg ttctgtggtg cctcatgaat acgctcgacc tcgtgccgga gccactccgt
     2161 gaccgcttcg tccctcggga gcgcgtggac gaggccgtgg agagggccac cgccttcctg
     2221 cgcgggtgcg tggcggagga cggctccctc ctctgggagg gcaacgagac ctcgacggcg
     2281 aagcacaaca tctggtgcta cgcgctcacc tatcatgtgc tccggcgcgc aggcggcgag
     2341 gagaatgagc aggcagcggc gcgcctcggg cgtttcctcg cttcgcggcg aacggcgagc
     2401 ggcctgcttc cgatgcgcga tcagggcgag gagatcaccg agtgcgcctt catgcaggcg
     2461 gatatgctcc tcttcctgct tcccttctcg gcactcgcct cgacggcgtg agcccggaat
     2521 tcatcaggca ttgtggccgc gacaaggcgg ctgtgacgat agagagagat accatccatg
     2581 ttgggaacac ccttgaaagg cagcgccacc cgtctgcttc tcctcggatc cggggagctc
     2641 ggcaaggaag tggcgattga ggcacagcgg ttgggcgtgg aagtgatcgc tgtggaccgg
     2701 tatccaaacg cccccgccat gcaggtggcg catcgcagcc atgtgatcaa tatgctggat
     2761 cgctctgagc tggcgcgcgt gattcgccag gagcgcccgg attacatcgt ccccgagatc
     2821 gaagcgatcg acacccgcta cctgctggaa gtggagcagg aggggttcac ggtcatccca
     2881 acggcgcgag ccacgaacct cacgatgaac cgggagggga tccgtcggct ggccgcggag
     2941 gagctgggcc t
//
LOCUS       DAOSCV010000570         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21654_length_2950_cov_3.832470, whole genome shotgun sequence.
ACCESSION   DAOSCV010000570 DAOSCV010000000
VERSION     DAOSCV010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21654_length_2950_cov_3.832470"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1580
                     /locus_tag="PLY56_13375"
     CDS             135..1580
                     /locus_tag="PLY56_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22517.1"
                     /translation="MVPYDSRMFIAVGLAVGLACAATVAAGEARKQMPEPLKPTVEIE
                     EDVYHFEPANNGSDPMWCWGSTCLTRAGGELFASGLETLKEYRPLNNVRWLLFRRGAE
                     GWERVAADEKGRTREPCPIVGFSDGQLFLSANPTLLTDPKAEGGGPARPEILRFDAKN
                     LKAGYETLLPEWDGKPPFTEHTYRTFAADGPGRELILFNNIGYDYAEWCFRDRDGRWA
                     ARGRLAWPPKEEPVEVAPYHEKSDRITYPNVVLKDRAVHLCGAAAYNTWSRVTTPDKM
                     GRNWGNRWRRLYYTWTPDILSRQWKEWVLVANTQENGGWLFPGDLWVGPDGTVHLLWY
                     EGPIGKALRDQFFPDIKRTWAWRYARIRNGKVVYRRTLLEGGEGINTDTAGETGGARF
                     QVTPDNRLFVIYFLVTTDESGKRVPSNRVMEILPDGSPGESVVLPLQHPLRGFFTATH
                     RGGSPPSTTVDLLGYRYGTQNQISYARVRLW"
     gene            1577..2629
                     /locus_tag="PLY56_13380"
     CDS             1577..2629
                     /locus_tag="PLY56_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22518.1"
                     /translation="MTSRESGEGNTMECGEEQVKARSAGLQQGRRMGRLAAWTAGAVA
                     AGMLLLGGASMAEKGRRLTGAWECVAEQAAFSTRDTAEGVIFQGKLWLSNGYHAGNIL
                     SRDLWSSTDCVNWTLVSDKTPYDGYSEMVVFQGKLWAIKGSVWNSTDGVTWERVSEKT
                     PFGTRGYGEIVVHDGKMWHLGSGKDVWWTTDGVQWTCATRDAPWGARWASAVAVHAGK
                     LWLIGGATQETSDPPEKYYPKFTTHNDVWCSEDGVNWTQVLEHAPWAKRMWFPAVVYA
                     GRLWVLGGFSNRRGENLNDVWYTEDGITWERFESPVVFSPRHEPTCYVFQDALWVVAG
                     NAWPLVNDVWRLRVAE"
     gene            2640..>2950
                     /locus_tag="PLY56_13385"
     CDS             2640..>2950
                     /locus_tag="PLY56_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22519.1"
                     /translation="MRIGWASVDITPDRPVVLRGQFHARISNRVNDPLLATALVLADE
                     GAGEHVVMVSCDRVGVPGGIAQRVREAVASRAKGLDVQRIFLSATLTHTAPEIEEGQY
                     PP"
BASE COUNT          510 a          841 c         1089 g          510 t
ORIGIN      
        1 tgaggggggc atcccctggg gctgctccca tccttctgag agcaagacgg gcgcgaccgg
       61 cgctcgcgct ggatcgagcc ggtggcagcc gctggcggtg agcggcgtgc cgttggcgga
      121 agggagcgag gcagatggtg ccgtatgata gccggatgtt catcgcggtc ggcctggctg
      181 tcgggcttgc ctgcgcggcg acagtcgcgg ctggggaagc gaggaagcag atgcccgagc
      241 ccttgaagcc cacagtggag atcgaggagg acgtttacca tttcgaaccg gcgaacaacg
      301 gctcggaccc gatgtggtgc tggggatcga cctgcctgac gcgggcgggg ggcgagcttt
      361 tcgcgagcgg cctggaaacg ctgaaggagt accggccgct gaacaacgtg cgctggcttc
      421 tcttccggcg gggtgcagag gggtgggaac gggtcgcggc ggatgagaag gggcgcacgc
      481 gcgagccgtg cccgattgtt ggcttctccg atggccagct ttttctctcg gccaacccga
      541 cgctgttgac cgatcccaag gctgagggcg gcggccccgc gcgcccggag atcctccgct
      601 tcgacgcgaa aaacctgaag gcgggctacg agacgctcct tcccgaatgg gatggtaagc
      661 ctcccttcac ggagcatacg tatcgcacct tcgcggccga tggcccgggg cgcgagctca
      721 tcctattcaa caacatcggt tatgactacg ccgaatggtg cttccgcgac cgcgacgggc
      781 gctgggccgc gcgcggccgg ctcgcctggc cacccaaaga ggagccggtg gaggtggcac
      841 cgtatcatga gaaatcggat cggatcacct acccgaacgt ggtgctgaaa gatcgcgccg
      901 ttcacttatg cggcgccgcc gcctacaaca cctggtcgcg cgtgacgacc ccagacaaga
      961 tggggagaaa ctggggcaac cgctggcgtc ggctctacta cacctggacg cctgatatcc
     1021 tttcccggca gtggaaagag tgggtcctcg tggcgaatac ccaggagaac ggcggctggc
     1081 ttttccccgg cgacctttgg gtggggccgg atggcacggt gcacctcctc tggtatgagg
     1141 ggccgattgg caaggcgctg cgcgaccagt tctttccgga tatcaagcgg acgtgggctt
     1201 ggcggtatgc ccgcatccgc aatggcaagg tggtctaccg gcgcactctc ctggagggtg
     1261 gggaggggat caacaccgat acggcgggcg agactggggg agcgcgcttc caggtgaccc
     1321 cggacaaccg gcttttcgtc atctacttcc tggtcacaac ggacgagagc gggaagcgcg
     1381 tgccatccaa ccgcgtgatg gagatcctgc cggacggcag cccgggcgag tcggtggtct
     1441 tgccgttgca gcacccgctg cgcggcttct tcacggcgac tcatcgcggg ggctcgcctc
     1501 cttcgacgac ggtggacctg ctgggctacc gctacggcac gcagaaccaa atcagctatg
     1561 cccgggtgcg cctctggtga cgagccgtga aagtggtgag gggaacacga tggagtgcgg
     1621 cgaagagcag gtgaaggcga gaagcgccgg tttgcagcag ggccggagga tgggccggct
     1681 ggcggcatgg accgcaggag cggtggctgc cggcatgctt ctcctgggag gcgcgagtat
     1741 ggcggagaag ggcaggcgcc tgacgggcgc atgggagtgc gtggcggaac aggcggcgtt
     1801 ctccacgcgt gatactgctg aaggcgttat ctttcaaggc aagttgtggc tgagcaacgg
     1861 ctaccatgcc ggcaacatcc tctcgcgcga cctgtggagc tccaccgatt gcgtgaattg
     1921 gacgctcgtc agtgacaaga cgccctatga tggctatagc gagatggtcg tcttccaggg
     1981 gaagctgtgg gcgatcaagg ggagcgtttg gaactcgacg gatggcgtca cgtgggagcg
     2041 ggtgagtgag aagacgccgt tcggcacgcg aggctatggc gagatcgtcg tccacgatgg
     2101 taagatgtgg cacctgggaa gcggcaagga tgtctggtgg acgaccgacg gcgtgcagtg
     2161 gacgtgcgcc acgcgggatg cgccatgggg agcgcgttgg gcctcggcgg tcgctgtcca
     2221 tgccggaaag ctctggctga tagggggcgc cactcaggag acgagcgatc cgcctgagaa
     2281 gtactatccg aagttcacca cccacaatga tgtctggtgc tccgaggatg gcgtgaactg
     2341 gacgcaggtc ctggagcacg cgccctgggc caagcggatg tggtttcccg cggtcgtcta
     2401 tgccggccgg ctgtgggtgc ttggcgggtt cagcaaccgg cggggcgaga acctgaacga
     2461 tgtctggtac accgaggatg ggattacctg ggaacgcttc gagtcgccgg tggtcttctc
     2521 gccgaggcac gagccaacct gctacgtctt ccaggacgcg ctgtgggtgg tggcagggaa
     2581 cgcctggccg ctggtgaacg acgtgtggcg attgcgtgtc gccgagtgag gagcgcgaga
     2641 tgagaatcgg atgggcatcg gttgatatta ccccggatcg gccggtcgtg ctgcgcgggc
     2701 agtttcacgc gcgcatctcc aatcgcgtga atgaccccct ccttgccacc gcgcttgtcc
     2761 tggcggatga gggtgcgggc gagcatgttg tgatggtctc gtgcgatcgc gtcggggtcc
     2821 cgggcgggat cgcgcagcgc gtgcgcgaag cggtggcctc gcgggcgaag gggctggatg
     2881 tgcagcggat cttcctcagc gcgacgctca cgcacaccgc gccggagatt gaagagggcc
     2941 agtatcctcc
//
LOCUS       DAOSCV010000571         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21656_length_2950_cov_3.330225, whole genome shotgun sequence.
ACCESSION   DAOSCV010000571 DAOSCV010000000
VERSION     DAOSCV010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21656_length_2950_cov_3.330225"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            627..1652
                     /locus_tag="PLY56_13390"
     CDS             627..1652
                     /locus_tag="PLY56_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22520.1"
                     /translation="MCVVAGYIGERQAAPLLLEMLEREEGLGGGFYTGIATIHEGRLY
                     TEKVVGDVAALRRQTDAEKLPGTIGIAHSRTPSGGDREWAHPFVDCTGQLAYIANGAR
                     GFFSETTNLAEAGRQLAAAGHKFRSICQEPVGNYPMVPEVGAVHMSDVMCHLIEDAYR
                     KSGSLVAAAAEAYQGWPGEIVGMCLHVNEPDHVVAMRINQPLVIGREGGETYLATTSL
                     AFPEPVSWRMPMAVNAAATVSRESVEIRHFTSARVPVAPMPSPLAVEQTVVNALREEG
                     LLGIGALCGRTKPIWPDGALGQDAMLVYETVATLVAEGRVRLETDLVPGMFGEGQVPR
                     TRVRWIA"
     gene            1710..2453
                     /locus_tag="PLY56_13395"
     CDS             1710..2453
                     /locus_tag="PLY56_13395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOJ22521.1"
                     /translation="MKMVTGEEERVSDLLRTSESFLRYVAEWEADLAVLPLAQVVREA
                     GGPGGVALHVVDLTVGFCDTGRLASPRIAAIVFPIVDLLCRAHDAGVKHFLLIQDAHP
                     EGSPEFEAFGPHCVVGTPEAKTVPELEALPFSNQFLRIPKRSISPSIGTELESWMAAH
                     PEVTRHIVVGDCTDICVYQTAMYLKMRANALMLEYEVLVPEDCVQTYDFSVERAQAIG
                     AIPHDGDLLHLIALYHMALNGVRVVRTIT"
     gene            2520..>2950
                     /locus_tag="PLY56_13400"
     CDS             2520..>2950
                     /locus_tag="PLY56_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22522.1"
                     /translation="MIELPERPSRPMDGTACGKQMEVVFLGTAGYHPNETRHTSCVMI
                     PEMGIVLDAGTGFFRVRQHLRATTLTILLSHLHLDHVDGLTYLLDVLWEKPVEAVTIH
                     GPPGVEYLRHGLFETPLFPLPLTYPVSPVPEEGMEIAGARVT"
BASE COUNT          479 a          940 c         1011 g          520 t
ORIGIN      
        1 ggccgtgtgt gcctcaatca gcgcgcgcat ggcataccac gggcgcctgc cccagtactt
       61 atgggccaga tagcggtgct ggaagtactt ctcgctcatg tgatagctct tcggccaccc
      121 catcgggcga ggcaagcgcc gctgtctccc gtggctggcg ttctcccctt tctctggtca
      181 gcaggcacac tcctgcgaga atcgtcacgc tgccggacat gctcacggat ccgcccggcc
      241 acaatgcgcc tgcctcgccg ggcgggagat ccccttcgca gtggcagaac taccggaggg
      301 aaggcgcggg cgactggccg gtctccaccc cccgatcccg gcggatggca acggcttcca
      361 gcacctggct tgtgctcgga aaggagcgcg gcgttccgcg gtgaactccc aatcaaccag
      421 ggtgaagaag cctgcgctcc tcagagggca cgggttgcac gcgccgggat ctgggcgaga
      481 gcgccaggca gggccttgcg gtgtcgtatc tgccggacgc tatcgcagag cagtcggaga
      541 gcacgcgcca gggagtatca ggtttggcgg atcgcgcttc gggtgttgcc acctgcggcg
      601 caacatttaa gacggtggag gttcccatgt gtgttgtggc agggtacatc ggagagcggc
      661 aggcggcgcc gctgcttctg gagatgctgg agcgagaaga gggcctggga ggcggcttct
      721 acaccggcat cgccacgatc catgaaggcc ggctctatac cgagaaggtg gttggagatg
      781 tcgctgcgct gcgccggcag accgacgccg agaaactgcc gggcacgata gggatcgccc
      841 atagccgcac cccgagcggt ggcgatcgcg aatgggccca ccccttcgtc gactgcacgg
      901 gccagctcgc ctacattgcc aacggtgccc ggggcttctt ctcggagacc accaatctgg
      961 ccgaggccgg ccggcagctc gcggcagcgg ggcataagtt ccgcagcatc tgccaggagc
     1021 cggtcggcaa ctaccctatg gttcccgagg tgggcgccgt ccacatgagc gacgtgatgt
     1081 gccacctgat cgaggatgcc taccggaaga gcgggagcct ggtggcggcc gcggccgagg
     1141 cgtatcaggg ctggcccggc gagatcgtcg ggatgtgcct ccatgtcaac gagccggatc
     1201 acgtggtggc gatgcgcatc aatcagcccc tcgttatcgg tcgcgaggga ggagagacgt
     1261 atctcgccac gacgagcctg gccttcccgg agccggtctc ctggcgcatg ccgatggccg
     1321 tgaacgccgc cgcgacggtc tcgcgcgaga gcgtcgagat ccgccacttc acttctgcgc
     1381 gcgtgccggt tgccccgatg ccctctccgc tcgccgtgga gcagacggtc gtgaacgcgc
     1441 tgcgcgaaga ggggctcctt gggataggcg ccctgtgcgg gcgcacgaag ccaatctggc
     1501 cggacggcgc gctggggcag gacgcgatgc tcgtctacga gacggtcgcc acgcttgtcg
     1561 ccgaggggcg ggtgcgcctg gaaacggatc tcgttcccgg catgttcggc gagggccagg
     1621 tgccgcggac gcgcgtgcgc tggattgcgt gaggttgttg ccggcgcccg tgaagccgtc
     1681 tgctcttgcc acgaggggag gttgcgacaa tgaagatggt caccggggaa gaggagaggg
     1741 tctcggatct actgcggaca tccgagtctt ttctgcgcta cgtggcggag tgggaggctg
     1801 acctggcggt tctgccactt gctcaggtgg tgcgcgaggc aggcgggccc ggcggggtgg
     1861 cactccatgt cgtggacctg accgtcggtt tctgcgacac gggccgcctg gccagcccgc
     1921 ggatcgccgc cattgtcttc ccgattgtgg atcttctctg ccgcgcgcac gacgcgggcg
     1981 tgaagcactt cctgctgatc caggatgcgc acccggaggg ctcgcccgag tttgaggcgt
     2041 tcggcccgca ctgcgtcgtc ggcactcccg aggcgaagac ggtgccggag ttggaggcgc
     2101 tgcctttctc caaccaattc ctcaggatcc caaaacgctc gatcagcccc tccatcggca
     2161 ccgagctgga gtcctggatg gccgctcatc cggaggtcac ccggcacatc gtcgttggcg
     2221 actgcaccga tatctgcgtg taccaaaccg cgatgtacct gaagatgcgt gccaacgccc
     2281 tcatgctgga gtacgaggtg ctcgttcccg aggactgcgt gcaaacctac gacttcagcg
     2341 tggagcgcgc gcaggcaatt ggcgcgatac cccatgatgg cgacctgctc cacctcatcg
     2401 cgctctacca catggcgctc aacggcgtgc gcgtcgtgcg caccatcacg tgaaggaata
     2461 gggcagggga gagcgggcag agttcgggct gccgctttgt gggtgggtgg tgccacccca
     2521 tgattgaact tccggagcgg cccagccgcc cgatggatgg gaccgcgtgc ggcaagcaga
     2581 tggaagtggt cttcctgggc acggcgggct atcacccgaa cgagacgcgg cacacgagct
     2641 gcgtgatgat ccccgagatg gggatcgttc tcgacgcggg cacgggcttc ttccgcgtgc
     2701 gccagcacct gcgcgcgacc acgttgacca ttctcctctc gcaccttcat ctggatcatg
     2761 ttgacgggct gacctacctt ctggatgtgc tttgggagaa accggttgaa gcggtcacga
     2821 tccacgggcc ccccggagtg gagtatctgc gccatggcct tttcgagaca cccctctttc
     2881 ccctgccgct gacctatccc gtctcaccgg tgcccgagga aggcatggag atcgccggcg
     2941 cgcgggtcac
//
LOCUS       DAOSCV010000572         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21673_length_2948_cov_4.049775, whole genome shotgun sequence.
ACCESSION   DAOSCV010000572 DAOSCV010000000
VERSION     DAOSCV010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21673_length_2948_cov_4.049775"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLY56_13405"
     CDS             complement(<1..1009)
                     /locus_tag="PLY56_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22523.1"
                     /translation="MALVVACAALCSTRSAEAQQASFWTDEALTTGWNAMSGSVPLFE
                     WGDTGWMRFQRQASPSWFVFRRGLYSAPIILSMRRQGTLSNWNDPRVFQVICSKSVVA
                     SRGADFATYDPRLSPARVGIDFGFYCAPGSDPSNPAKWTLGRAQLAKSFTPGTPPVVA
                     ELPAAGFWIDPDNKPEAVLTPGVWEIRVHQNEDFADRNGRFTDFYPEPITNDVNGDGR
                     YDYPEPFNDLNGNGVWDEGEPFLDMGNGWYDPPEPFVDENGNGVWDHDDTNGNGIVDD
                     GEDHEPFTDWNNNKRCDPGEPFNDANGNGYWDPPEPFADYNGNNTRDPDEPFMDLNGN
                     GV"
     gene            complement(1069..>2948)
                     /locus_tag="PLY56_13410"
     CDS             complement(1069..>2948)
                     /locus_tag="PLY56_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOJ22524.1"
                     /translation="PTVSGGVVYVAAGDGRLFALDADPRQGARGAEFILVDDGIADAA
                     GAGWLRQDRVLPRSWTPRVEPGYLRVPPDLLGVWPDADPQGQPVIGRRPLIFGGATAT
                     SAMLLLGRNEVQPDGTFRGSTVYALSRPVYTVADAKRMLELDGEGRVLSGMSEARDDT
                     AERALNHPAAGSIAGHFSSDGQATLFADTGNHRVVAVDRAGQVVWEAREVYDFMNLLP
                     QGEYEVVSELRRPLVPFTDLNRDNQPDLFTATGTVRVSKLQTPADARSWVTYETDGDG
                     QRIKVWHTLIADSGNYRVMEIVDKWVWNAAGQRWDRYRLTDPAHGNPYWYHVVVWASQ
                     TRARHRDASGQWQMGQNYRYTSAQVVEYAQGYARRVLCTVSNYALYRAGGRRHQRAGA
                     SLVMLAPNPAATGADVGKAYIVDDPQAPPGYTCAIGNVANPSQPRYLNGMGTIQRYLY
                     GWTAATGPVWHYIYADNTGIYEWRPAAGQPLQLPLDADGNLDATSVRSQFWREHYEKF
                     LYDPANPDWLPGGDPRQFQPGAVSVDPAGGWIVINRVFDRRLSQALRQRGRLDEMRRI
                     QVFKIASTPWPAGDYNPGTANFGYGASSIRWLFGPQLLDLRYRDLKVDLAQPMYAVRS
                     R"
BASE COUNT          503 a         1020 c          918 g          507 t
ORIGIN      
        1 aaacgccatt cccgttgagg tccatgaacg gctcatcggg atcgcgcgtg ttgttgccgt
       61 tgtaatccgc gaacggctcg ggcgggtccc aatagccgtt gccgttggca tcgttgaacg
      121 gctcgcctgg atcgcaacgt ttgttgttgt tccagtcggt gaacggctcg tgatcctcgc
      181 catcatccac gatgccgttg ccgttggtgt cgtcgtgatc ccatacgccg ttgccgttct
      241 cgtccacgaa cggctcgggc gggtcgtacc agccgttgcc catatccagg aagggctctc
      301 cctcgtccca aacgccgttg ccgttgagat cattgaacgg ctcgggatag tcataccgcc
      361 cgtcgccgtt tacgtcattg gtgatcggct ccgggtagaa atccgtgaac ctgccgttcc
      421 ggtcagcgaa atcctcgttt tgatgcacac ggatttccca gacgcccgga gtgagcacgg
      481 cctccggctt gttgtccggg tcaatccaga accccgccgc cggtagctcg gcgacaaccg
      541 ggggggtgcc cggcgtgaag ctcttggcga gctgcgcccg gcccagggtc cacttggcgg
      601 ggttgctggg gtcgctgccg ggggcgcaat agaacccgaa gtcgatcccc acccgcgcgg
      661 gcgagagccg cgggtcgtag gtagcaaagt ccgcgccgcg ggacgcgacg acgctcttgc
      721 tgcagatcac ctggaacacc ctgggatcgt tccaattcga cagggtgccc tggcgacgca
      781 tcgacaggat gatgggcgcg gagtagagcc cccggcggaa gacaaaccac gagggcgaag
      841 cctgccgctg gaatcgcatc cacccggtat cgccccactc gaagagcggc acggagccac
      901 tcatggcgtt ccagccggtg gtgagcgctt catccgtcca gaagctggct tgctgagcct
      961 cggcggatcg ggtggagcat aacgccgcgc aagccaccac cagggccacg atggcccacc
     1021 ctcgcttacg ctctctcctg ccttgcctca cgtctacctc ccacgctctc accgcgatcg
     1081 caccgcgtac atcggctgag cgagatccac cttcaagtct ctatagcgca gatcgaggag
     1141 ctggggcccg aaaagccacc gaatgctgct cgctccatat ccgaaattgg ccgtgcccgg
     1201 attgtaatcg cccgcgggcc acggcgtgct cgctatcttg aacacctgga tgcgccgcat
     1261 ctcatccagc cggccgcgct ggcgcagcgc ctgagacagc ctgcggtcaa acacccggtt
     1321 gatcacaatc cagccgccgg cgggatcgac cgagacggcg ccgggttgga actggcgcgg
     1381 gtcgcccccc ggcagccagt caggattcgc cgggtcgtac aggaacttct cgtagtgctc
     1441 gcgccagaac tgactgcgaa cagaagtggc atccaggttg ccatcggcat ccagcggaag
     1501 ttgcagcggc tggcccgcgg ccgggcgcca ctcgtagatc ccggtgttat cggcatagat
     1561 atagtgccac accggcccgg tagccgccgt ccacccgtag agatagcgct ggatcgttcc
     1621 catcccgttc agataccgcg gttgcgaggg attggcgaca ttgccaatcg cgcacgtata
     1681 gcccgggggc gcctgcgggt catccacgat gtaggctttg cccacgtcgg caccggtcgc
     1741 ggcaggattg ggcgccagca tcaccagaga cgcgcccgct cgctgatggc gcctgcctcc
     1801 cgcccggtag agcgcgtagt tagagacagt gcacagcaca cggcgcgcgt agccctgagc
     1861 gtactccacc acctgcgcgc tggtgtagcg gtagttctgc cccatctgcc actggccgga
     1921 tgcatcccgg tgcctcgccc gcgtctgcga cgcccatacc accacgtgat accagtaggg
     1981 attgccatgc gcgggatcgg tgagccgata gcgatcccag cgctgccccg ccgcgttcca
     2041 gacccattta tccacgatct ccatcacgcg gtagttgccc gagtctgcga tcagcgtgtg
     2101 ccacaccttg atccgctggc catccccgtc cgtctcatac gtcacccacg agcgcgcgtc
     2161 cgctggcgtt tgcagcttcg agacgcgcac cgtgccggtg gcagtgaaga ggtccggctg
     2221 gttatcgcgg ttgaggtccg tgaatggtac aagaggcctg cggagctcgc tcaccacctc
     2281 gtactcgccc tgcgggagca ggttcatgaa gtcatagacc tctcgggcct cccagacaac
     2341 ctgtccagcc cggtccaccg ccacaacgcg gtggttgccc gtatccgcga aaagcgttgc
     2401 ctggccgtcg ctagagaaat gcccggcgat gctccccgcc gcggggtggt tcagagcgcg
     2461 ctccgccgtg tcgtcgcgcg cttccgacat cccggagagg acccggcctt cgccatcgag
     2521 ctccagcatg cgcttcgcgt ccgcgacagt gtaaacgggg cgcgacagcg catacaccgt
     2581 gctgccgcgg aatgttccat cgggctgcac ctcgttgcgc cccaggagga gcatcgccga
     2641 ggtggccgtc gcgccgccaa agatcagcgg ccgccggcca ataacaggct ggccttgcgg
     2701 atcggcatcg ggccaaacgc ccagcaagtc aggcggcacc cggaggtagc cgggctccac
     2761 ccgtggagtc cacgaccggg gcagcacgcg atcctggcgc agccatccgg cccccgcggc
     2821 atccgcaatc ccgtcatcca ccaggataaa ctcagcaccg cgtgccccct ggcgcgggtc
     2881 cgcgtccagg gcgaagagcc ggccatcccc cgcggccacg taaacgacgc ccccggagac
     2941 cgttgggg
//
LOCUS       DAOSCV010000573         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21695_length_2945_cov_3.910381, whole genome shotgun sequence.
ACCESSION   DAOSCV010000573 DAOSCV010000000
VERSION     DAOSCV010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21695_length_2945_cov_3.910381"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLY56_13415"
     CDS             complement(<1..1389)
                     /locus_tag="PLY56_13415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ22525.1"
                     /translation="MTFRFLRLFMLVCGAALAHLAMAPVPAHAGRILDPATITQTTLP
                     NGLRVVIKEAPSIPLVALNVWVRAGSSDETEENNGVSHFLEHLMFKGSKKRGPGEFDL
                     EIEGLGGLANATTMEDATQYYVVVAQQYALQALEALGDMISNPTFPEEELRRERQIIL
                     NEVAQAAEDPQRILSEWINRLSFQSHPYRLPIAGTPQSIRQITREKIQAFYDKYYVAG
                     NMTLVVVGAVKAAEVLPQIEAAFAGLRTGPVPARDYSMEPIPEKVRFSTLERPVSGAS
                     LVFGFRAPGMDAKDDVCAMDVLLYILGEQRAQSGRLNRELRQKQNLVSAIWSDYVTLR
                     HPGIVTFWAETEPAKLEAARAAILEQIAQVRDTLVPDDEIQRAKMLLLGVYTMDNETY
                     DGQAGTLGFYEAKDTYQFALEYEERIARVTAADIQRVARKYLGENSYCLAVLRPKQSE
                     GGSIATGTETRSI"
     gene            complement(1554..2564)
                     /locus_tag="PLY56_13420"
     CDS             complement(1554..2564)
                     /locus_tag="PLY56_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22526.1"
                     /translation="MKTLALLLLISAGSLAVTVERPELRSVRRRRRLATLAVALVILA
                     GLLYPEYIRWRRAAYDQGAVELLRSLHKAEAQHYKRHGTYLPLDKLKAAGLSPMGEGD
                     IFEGYSYTSTASEKGYTIVARPTWPEGQSYTLDTSGYLNRPAPQERAKTPEEKLALAR
                     EEARRAPQDAAAWVRLSEALAVTGRYAEALSAARRASSLNPTAARQALSDALVYRAGE
                     LLELKKPRTAIPLLTECIRLDENDADALALLGDAYQALGRDTEALQAYRKATQSGLSS
                     ARAWLGLARMLEARGQVKEAAQAYLYVVDIGDETHAPDAWVHEATMALKRIHGQEDAR
                     GR"
BASE COUNT          503 a          933 c          964 g          545 t
ORIGIN      
        1 gatgctgcgc gtctcggtgc cggtggcgat gctgccgccc tcggattgct tggggcgcaa
       61 gacggcgagg caatagctgt tctcgcccag gtatttgcgc gccacgcgct ggatatctgc
      121 ggcggttaca cgcgcgatcc gctcctcata ctcgagcgcg aactggtagg tatccttcgc
      181 ctcgtagaag ccgagcgtgc ctgcctggcc gtcatacgtc tcgttatcca tggtatagac
      241 gcccaacagg agcatcttcg cccgttgaat ctcatcatcg ggcaccagcg tgtcgcgcac
      301 ctgggcgatt tgctccagga tcgccgcgcg tgccgcctcg agcttggcgg gctccgtctc
      361 cgcccagaag gtcacgattc cggggtggcg gagggtgacg tagtcgctcc agattgccga
      421 aacgaggttc tgcttctgcc ggagctcccg gttgagccgg cccgactgcg cgcgctgctc
      481 tcccaggatg tagaggagca catccatcgc gcagacgtcg tctttcgcgt ccatgccggg
      541 ggcgcggaaa ccgaatacca aggatgcgcc cgatacgggc cgctccagcg tgctaaagcg
      601 caccttctcc gggattggct ccatggaata gtcgcgggcc ggcacggggc cggtccgcag
      661 cccggcgaac gctgcctcaa tttgcggtag cacctcggcg gccttcacgg cacccaccac
      721 gaccagtgtc atattgccgg ccacgtagta cttatcatag aaggcctgga tcttctcccg
      781 ggtgatctgc cggatcgatt gcggcgtgcc ggcgatagga aggcgatagg gatgactctg
      841 gaaggagagc cgattgatcc actcggagag gatgcgttgc ggatcctccg cggcctgggc
      901 cacctcattg aggatgatct gccgctcgcg ccgcagttcc tcctcgggga aggtgggatt
      961 ggagatcata tcgcccaacg cttcgagcgc ttgcaaggca tattgctggg ccacgacgac
     1021 ataatactga gtggcgtcct ccatcgttgt ggcattcgcc aagccgccca gaccctcgat
     1081 ctccaggtcg aactcgcccg gccctcgttt cttcgaacct ttgaacatca ggtgctcgag
     1141 gaaatgcgaa acgccgttgt tctcctcggt ctcatctgaa gagccggcgc gcacccagac
     1201 gttcagagcc acgagcggga tgctgggtgc ctctttgatc acaacgcgca ggccattggg
     1261 aagcgtcgtc tgggtgattg tggcggggtc gaggatccgg cccgcgtgcg cgggcacggg
     1321 ggccatcgcc agatgagcca gagcggcgcc gcagaccagc atgaagagac gtagaaaacg
     1381 aaaggtcacc gcggtctcct attcagatga agcgttgtga atgcccaaag ccggggcgcc
     1441 tgcggaggag gcggccaccg gctggttgga acagcccgtg gcctgtggat agaccgatag
     1501 gactgccgag gccattatag catgtttgcc aaacaccgtc tagcaacacg gggtcaccgt
     1561 ccgcgcgcgt cttcctgccc gtgaatccgc ttcagggcca tcgtggcctc gtggacccag
     1621 gcatccggcg cgtgcgtctc gtcgccgata tcgaccacat agaggtaggc ttgagccgcc
     1681 tccttcacct ggccgcgcgc ctccagcatc cgggcgaggc cgagccaggc acgggcgctg
     1741 gagaggccac tctgggtggc cttgcgatag gcctggaggg cctccgtatc gcgtccgagc
     1801 gcctgatagg catcgcccag gagcgcgagg gcgtcggcgt cgttttcatc caggcggatg
     1861 cattccgtga gcagggggat agccgtgcgc ggcttcttca gttccagcag ctcgcccgcg
     1921 cggtagacga gcgcgtcgga gagagcctgc cgggccgcgg ttgggttcag gctcgaggcc
     1981 cggcgcgcgg cggaaagcgc ctccgcatac cgccctgtca ccgcgagtgc ctccgagagg
     2041 cggacccagg ctgctgcgtc ttgtggcgcc ctccgggcct cctcgcgcgc cagcgccagc
     2101 ttctcctccg gtgtcttcgc gcgctcttgc ggcgccggcc gattcagata gccggaggtg
     2161 tcgagtgtgt aggattgacc ctcgggccac gtcggccggg caacgatcgt gtaccccttc
     2221 tcgctcgcgg tgctggtgta ggagtaacct tcgaagatgt cgccctctcc catgggagag
     2281 agacccgcgg ctttcagctt atcgagcggc agataggtgc cgtgcctctt gtagtgctga
     2341 gcctccgcct tgtgcaggct ccggagcagc tccaccgctc cctggtcgta ggccgcgcgg
     2401 cgccaccgga tgtattccgg atagagcagc ccggccagga tgaccagcgc aaccgcaagc
     2461 gtggcgaggc ggcgccggcg ccggaccgat ctcagttcag ggcgctccac cgtaaccgcc
     2521 agcgaaccag ccgagatgag taacagcaat gcaagtgtct tcatggaaca atgcgtctgg
     2581 gcgatcctcc ccgggtagag ttggcattcg gacgccccac tccttccgac gtgcgcgaaa
     2641 gagcgaaggt tcgcatagag gcgcgaacaa actattggct acgcggtggc cccctccgtc
     2701 gataggagag cctgcccatg ggcatggttc catctgccgg ctgcctcctt cccatacgag
     2761 aagatctcgc gccttcccag ataaaggagc agcgtgtttt gcaggggctg gctcgtccgg
     2821 catagaagga gataccgcta acgcggcacc cggccgctat tggagggaga accacgatga
     2881 gagcatggca gagttgcgcg cgagcgcggg ggttcaccct gatcgagttg cttgttgtca
     2941 tcgcg
//
LOCUS       DAOSCV010000574         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21742_length_2940_cov_3.755286, whole genome shotgun sequence.
ACCESSION   DAOSCV010000574 DAOSCV010000000
VERSION     DAOSCV010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21742_length_2940_cov_3.755286"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PLY56_13425"
     CDS             <1..491
                     /locus_tag="PLY56_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22527.1"
                     /translation="RPWWQTAPEAPPDHSLPDSTGRVQEWQPSPYPEEPGFGHGWPDR
                     RPYRPRRERVRRAPAPIPPGRDLPYYPPEPPPSAPPSSAVSAPPDFIGSATGILEIPV
                     GQTATLFVSDYQEATTREERIACVVARESDRVLIRGRFPGRTLLEVRQGARRDIYLVR
                     VR"
     gene            complement(499..2310)
                     /gene="mgtE"
                     /locus_tag="PLY56_13430"
     CDS             complement(499..2310)
                     /gene="mgtE"
                     /locus_tag="PLY56_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOJ22528.1"
                     /translation="MLLPGQPLYLSRLLGSRVVERDGVRIGWVADVVARPGGLFPVVV
                     GLLVRRPGARMLRVPWECLDRVSEGSVALAGPVAEVAPLEVQEGDILLGAEVLDKQLV
                     DMHGRRVVKVNDLRLIPVRGDLRVVGVDVGIRGILRRLGMEDATERFAGLMRRSLPEA
                     LIPWNYVESVPTRGASVRLSVESSRLERLPPADISRIISQLSEEDRAAAVEQLSDEAI
                     AAAMAELDAPLQLSLMEGMDNARASDILERMAPDEATDLLQELPEERQAELLTLMERE
                     EAAELAELMQYDEHTAGGLMTTQFIAIPSHFTASEAIAQLREMAPGAETIYYLYVVDA
                     DGRLMGVLSLFRLITCAPDRPISEVMDTAVISVPANLDQEEVAATMVKYHLLALPVVG
                     DRGELLGIVTVDDTIDVMDEEAEEDVAHIAGTPAESPEERQRPAAVALTRAPWLLLAL
                     TAGLLAASTLHRVAGTALSQAIGFLPLALLLGNQIAARAAAATARGLALREVDRDTWW
                     SHSLAELRGSAVIALVTGFLSAAIAGLWGGGLLGAVVGCSLVCVVLSASVIGLSIPLL
                     EASVRLDPAVALRPIVISILAVMAPAIYAGFASLFLR"
BASE COUNT          543 a         1067 c          892 g          438 t
ORIGIN      
        1 gccgcccttg gtggcaaacc gcgcccgagg cgccgcctga tcactccctg ccggactcca
       61 cagggcgtgt tcaggagtgg cagccatcgc cctacccgga agagcccggg ttcggacacg
      121 gctggccgga ccgacggccc tatcggccca ggcgcgagcg cgtgcgccgc gctccggcgc
      181 caatcccccc aggtcgcgac ctcccctact acccgccgga gcccccgcct tccgcacccc
      241 cgagcagcgc cgtgagcgcg cctcccgatt tcatcggaag tgccacaggc atcctggaga
      301 tcccggttgg ccaaaccgcc acgctcttcg tgagcgacta ccaggaggcg acaactcgcg
      361 aggagcggat cgcctgtgtc gtcgcgcgcg agagcgaccg cgtgctgatc cgcggccgct
      421 tcccaggccg gacccttctg gaagtgcgac agggagcgcg acgcgatatc tacctcgtgc
      481 gcgtgcgctg agaacgggtc agcgcaggaa gagactcgcg aagccggcgt agatggcggg
      541 cgccatcacc gcgaggatgc tgatcacgat cggtcggagg gcaaccgccg ggtcgaggcg
      601 aaccgatgcc tccaggagcg gaatggagag gccgatcacg ctcgcggaaa gcaccacgca
      661 gacgaggctg caccccacca ccgcgccgag caatccgcct ccccatagcc ccgcaatcgc
      721 ggcgcttaag aatccggtca cgagcgcgat caccgcggaa ccgcgcagct ccgccagcga
      781 gtgcgaccac caggtgtcac gatccacttc acgaagcgcc aatccgcgcg ccgtagccgc
      841 ggcagctcgc gccgcgatct gatttcccag gagcagtgcc aggggcaaga agccaatcgc
      901 ctgcgacagc gcggtacccg caacccggtg gagcgtgctc gcagcaagta gcccggcggt
      961 aagcgccagg agcagccacg gcgctcgcgt gagcgccacc gccgccggcc gctgccgctc
     1021 ctccgggctc tcggccgggg tacccgcgat atgcgccacg tcctcctccg cctcttcatc
     1081 catcacatcg atggtgtcgt ctaccgtcac gatccccaac agctcgccac gatcgccgac
     1141 caccggcagc gccaggaggt gatacttgac catggtggcc gccacctcct cctgatccag
     1201 gtttgccggc accgagatca ccgcggtatc catcacttcg gagataggcc ggtcgggcgc
     1261 gcaggtgatt aggcggaaga gcgaaagcac ccccatcagc cgcccgtccg catcgaccac
     1321 atagaggtaa tagatcgtct ctgctccggg ggccatctcg cgaagctggg caatcgcctc
     1381 ggaggccgtg aagtgggagg gaatggcgat gaactgcgtg gtcatcaagc cgccggcggt
     1441 gtgctcgtca tactgcatca gctcggccag ctccgccgct tcctcgcgtt ccatcaacgt
     1501 gagcaactcg gcctggcgct cctcgggaag ctcctggaga aggtcggtcg cctcatccgg
     1561 ggccatccgc tccaggatat cggaggcacg cgcgttatcc atcccctcca tcaaggagag
     1621 ctgtagcggc gcgtccagct ccgccatcgc cgcggcaatc gcctcatcgc tcagctgttc
     1681 gacggcggcc gcgcggtcct cctcggaaag ctggctgatg atccggctga tatccgccgg
     1741 cggcaggcgc tcgagccgac tcgactcgac ggagaggcga acgctggcgc cgcgagttgg
     1801 cacagactcc acgtagttcc agggaatgag cgcctcgggc agcgagcgtc tcatcaggcc
     1861 cgcgaaacgc tcggtcgcat cctccatgcc caggcgacgc aagatccccc ggatgcccac
     1921 atcgacaccg acgacgcgga gatcaccgcg caccgggatc agccgcaagt cgttaacctt
     1981 cacgacgcgc cggccatgca tatcgaccag ctgtttgtcg agcacctccg cgcccaggag
     2041 aatgtcgccc tcctgtacct cgagtggcgc cacctccgcg acgggccccg ccagcgccac
     2101 cgacccctcg ctcacccggt ccaggcactc ccagggcact cggagcatcc gggcaccggg
     2161 ccggcgcacc aacaagccca ccacgaccgg gaagaggccg ccaggccgag ccaccacatc
     2221 cgcgacccaa ccaatgcgca cgccgtcgcg ctccaccacg cggctaccca ggaggcgtga
     2281 caagtagagt ggctggccag gcaacagcat ttcctctctt catggcagta gagtacacgg
     2341 tcggcagagg cacgcgccta cctaaagcaa tattgtacac ctaggtgtat tggcggtcaa
     2401 tgggtattat ggagattaca cctcggagta ttggtggtca ataccccaag gtgtattgac
     2461 cgccaactag tactccggca ttttggggat acggatgaag gaggcagccg cggatggccg
     2521 cccgcacgcg cggagctcaa agctccgcgc tgcttcccgg ctaagccctt cgggcttccc
     2581 atcccagcca ccgacgtttt gagacatctg ggagtatccc caacccagcc cgatagggct
     2641 tcgctctcta gcagcgcggt gatttaccgc cgcgcgtcgg gttgcgcgct gccctcctgg
     2701 gattccttat gtcggaatcc caggagggca ccccggcgct tgactctggg caccgggtgg
     2761 cctataatgg cgcccgtccg gcattccgcc ggcttccatc gggaaggaag atcacgaatg
     2821 gcactgcaac ccccactcga cacggaggag acccgcgccg aacgccccct cttccgtttc
     2881 gcgcaggtca ccgataccca catcatcgac gatcagagcg cggagatcct tcgcgccacc
//
LOCUS       DAOSCV010000575         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21844_length_2931_cov_4.349791, whole genome shotgun sequence.
ACCESSION   DAOSCV010000575 DAOSCV010000000
VERSION     DAOSCV010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21844_length_2931_cov_4.349791"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1654
                     /locus_tag="PLY56_13435"
     CDS             98..1654
                     /locus_tag="PLY56_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22529.1"
                     /translation="MLQARGPEKWTRAQARFDYFLSRFVHLDDRGGVKFKPWRWQVEL
                     AAELPRIRYLIILKARQIGMTWIFSAYGLWVALRPGSKCLCVSQGEDEAKDFLAKSQF
                     IFENLPAEAQPPHKALTEALIFPRERSFIRAMPSTAKAGRSVSAARLVMLDEFSNHMR
                     AADVMSAIAPSVESNPDGQLFVFSTANGVGNGYHAQWTAATQRRGWVEPYYDEALGEF
                     TFGDRLKRAVDETGPGEWLALFLPYNVRPGRDEAWYERERESATYHASPKKFYQEFPR
                     HVDEAFIQTGRPVFDLADLRKQEPWLRDPLPVDQWPEAFRTGRIRFDPRELRVFEAPK
                     QGAVYYAGADVAEGLEHGDYSDLSVFDKATDREVLSLHGHWPPDVFAALIDQVARVYP
                     GLYGIERNNHGLTVLTECDKHLHTPGIYREQAQFDPRTGAELSPGKLGWVTNSVTKPI
                     MVDELEIALRLGSIHLSDALALPELTYYQTFEDGSTGAPAGMHDDRVISRAIAWQMRK
                     QRGKRQMKVT"
     gene            1663..>2931
                     /locus_tag="PLY56_13440"
     CDS             1663..>2931
                     /locus_tag="PLY56_13440"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOJ22530.1"
                     /translation="MARPSLIARMGAAVKAFWNQVPMQGGTGVPELRALSGLESGVVL
                     TQDAGDQGDVGQPWANSAVMICLGWILRTFSEARFRVMIGEGEDAQEVRNHPLPQLLR
                     RPNPYYSGNALWAATNISYHTDGNAYWLIFRDGAGVPRELWYAPHWMMEPRWPKDGSQ
                     FVSHYEYTVGNTKHTLPPEDVVHFRYGLDPENTRKGMAPLKAGLKEIGADNLATVYSK
                     ALLRNTGAIPGIISPADPNTEITDDEREKLLQLYRSRVAGKHTGLPLVAAYGMRFEKL
                     ALSPEEMVLDRIRQVPEARIAALIGVAAMTVGLSVGENQRTYSNQAEAREASYETCII
                     PSHVVYCNELDLQLLPLLGRPDEEWCDFDYSRVRCLQEDQDALHRRAGLDYQNGIITR
                     GEARAMIGRKATPEDDVYFVQPQGRPEPLPA"
BASE COUNT          523 a          963 c         1011 g          434 t
ORIGIN      
        1 attgatggcg cgccggtcga ggtgaagcag gtcgggctgg acctcagcaa gctgtcggac
       61 gaggaactcg atgccctcga cgcaattgcg gagaagtgtg cttcaggcga gggggccgga
      121 gaagtggacc cgggcgcagg cgcggtttga ctacttcctc tcccgcttcg tccacctgga
      181 cgaccgcggc ggtgtgaagt tcaagccgtg gcgctggcag gtggagctgg cggccgaact
      241 accacggatc cggtatctca ttattctgaa ggcccggcag atcgggatga cctggatctt
      301 ctccgcctac ggcctgtggg tggcgcttcg gcccggcagc aagtgcctgt gcgtgtcgca
      361 gggcgaggac gaggccaagg acttcctggc gaagagccag ttcatcttcg agaacctgcc
      421 agccgaggcg cagccgcccc acaaggcgct caccgaggcc ctgatcttcc cgcgggaacg
      481 ctccttcatc cgggcgatgc catctaccgc gaaggccggc cgcagtgtgt cggcagcgcg
      541 cctggtgatg ctggacgagt tcagcaacca catgcgggcc gccgatgtga tgtcggcgat
      601 tgccccatcg gtggagagca acccggacgg gcagctcttc gtcttcagca ctgccaatgg
      661 cgtgggcaac ggctaccatg cgcagtggac cgcggccacg caacggcgcg gctgggtcga
      721 accctactac gacgaggcgc tgggcgagtt caccttcgga gaccgcctga agcgggccgt
      781 agacgagacc gggccgggcg agtggctggc gctcttcctg ccctacaacg tccgcccggg
      841 ccgcgacgag gcgtggtacg agcgagagcg tgagagcgcc acctaccacg ccagccccaa
      901 gaagttctat caggagttcc cgcgccacgt tgacgaggcg ttcatccaga cgggccggcc
      961 cgtcttcgac ctggccgatt tgaggaagca agagccgtgg ctgcgcgatc ccctgccggt
     1021 cgaccagtgg ccggaagcgt tccgcaccgg caggatccgc ttcgacccga gagagctgcg
     1081 cgtcttcgag gcgccaaagc agggcgcggt ctactacgcg ggcgcggacg tggccgaggg
     1141 cctggaacac ggcgactata gcgacctgag cgtgttcgac aaggcgaccg accgcgaggt
     1201 cctgagcttg cacgggcact ggccgccgga cgtgttcgcg gccctcatcg accaggtggc
     1261 gcgggtgtat cccggcctct acggtatcga gcgcaacaac cacggcctca ccgtgctcac
     1321 cgagtgcgac aagcacctgc acacgccggg catctaccgg gagcaggcgc agttcgaccc
     1381 aaggaccggc gcggagctgt cgcccggcaa gctgggctgg gtgaccaaca gcgtcaccaa
     1441 gccgatcatg gtggacgaat tggagatcgc cttgcgcctg ggcagcatcc acctctccga
     1501 cgcgctggcg ctccccgagc tgacctacta ccagaccttc gaggacggta gcacaggggc
     1561 gccggctggc atgcacgacg accgggtgat tagccgggcg attgcctggc agatgcgcaa
     1621 gcagcgcggc aaacggcaga tgaaggtcac atgagtacgg ctatggccag accctctctg
     1681 atagcccgga tgggcgcggc ggtcaaggcg ttctggaacc aggtccccat gcagggcggc
     1741 accggcgtgc ctgagctgcg ggcgctctcg ggactggaat cgggcgttgt gctgacccag
     1801 gacgccggcg accagggcga cgttggccag ccgtgggcca actccgctgt gatgatctgc
     1861 ctgggctgga tcctgcggac cttctccgag gcgcgcttcc gggtgatgat cggcgagggc
     1921 gaggacgccc aggaggtgcg aaaccacccg ctgcctcagc tcctgcggcg cccgaacccc
     1981 tactactccg gcaacgccct gtgggcggcc acgaacatca gctaccacac cgacggcaac
     2041 gcctactggc tgatcttccg cgacggggcc ggcgtgccgc gcgagctgtg gtacgcgccg
     2101 cactggatga tggagccgcg gtggccgaag gacggcagtc agttcgtcag ccactacgag
     2161 tacaccgtcg gcaacaccaa gcacacgctc ccgcccgagg acgtggtcca tttccgctac
     2221 gggctagacc cggagaacac gcgcaagggg atggcgccgc tgaaggccgg cctgaaggag
     2281 atcggcgccg acaacttggc aacggtgtac agcaaggcgc tgctccgcaa cacgggagcg
     2341 atcccgggca tcatctcgcc ggccgatccc aacacggaga tcaccgacga cgagcgcgag
     2401 aagctcctgc agctctacag gagccgtgtt gctggcaagc acactgggct gccgctcgtg
     2461 gcggcatacg gcatgcggtt tgagaagctg gccctctccc ccgaggagat ggtcctcgac
     2521 cggatccgcc aggtgccgga ggcccgtatc gctgccctga tcggcgtggc ggcgatgacc
     2581 gtcggccttt cggtgggcga gaaccagcgc acctactcga accaggccga ggcccgcgag
     2641 gcgagttacg agacgtgcat catcccctcc cacgtcgtct actgcaacga gctggacctg
     2701 cagctcctgc cgctgctggg gcggcctgac gaggagtggt gcgacttcga ctattcccgg
     2761 gtgcgctgcc tgcaggaaga ccaggacgcc ctgcacaggc gggcggggct ggactaccag
     2821 aacggcatca tcacccgggg cgaggcgcgg gcgatgatcg gccgcaaagc gacgcccgag
     2881 gacgatgtct acttcgtgca gccgcaaggg cggccggagc cgctgccggc a
//
LOCUS       DAOSCV010000576         2931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21846_length_2931_cov_3.460362, whole genome shotgun sequence.
ACCESSION   DAOSCV010000576 DAOSCV010000000
VERSION     DAOSCV010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21846_length_2931_cov_3.460362"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="PLY56_13445"
     CDS             <1..908
                     /locus_tag="PLY56_13445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOJ22531.1"
                     /translation="AAEDPACNYFSWVERLQDSRRTQWVLHRTPIRVSEILRTALWEN
                     VDTAVLTSATLATGGSFSYLRRCLGLPPAREIIVGSPFDYRRQACLYIAAHLDPPTEG
                     ESYQNAVAAEILRILELTRGRAFVLFTSYRALERAYAVVAPAVTFPCLRQGEASNAHL
                     LQEFRSTPNACLFGVQSFWEGVDVPGDALSCVIMDRLPFAVPDHPVHRARVQAIKEEG
                     GDWFNDYVLPQAQIRLKQGFGRLVRTRTDTGIVCILDSRLVRKAYGRAFLHSLPRCSI
                     TTDIRAVARFLQERDPSGGVQKKGR"
     gene            complement(1075..>2931)
                     /locus_tag="PLY56_13450"
     CDS             complement(1075..>2931)
                     /locus_tag="PLY56_13450"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ22532.1"
                     /translation="AHVEPPPGTLPIFFLEGTRLRPVYRPKGPATPAHALDALLAGPT
                     VEEQKGSLTTALPPGTRLLGLTRRGSETVVDLSHAIAPEALRVAVAQVTATLASFPEI
                     RAARILVQGTPVRAVLAAQFLHLGTAAANRAPAGPIHAAQSPAEASLPRGPLSGKVIV
                     ISPGHGLTRQSNGRWRYQRPFFEGEVEDQLNIAFATHACRELRRLGATVHTTRAPEGA
                     NEPGISGAPRWMEAAKYYLRDIGMPDWVHQPLPVDFDSDIDARPLYANYLGADLLISL
                     HSNIGPNTGTLTLYDKTNGFERESHRLARLLEAHVVRAIREKVDPGWRSHGAVGTDAR
                     YGENHWAHMPSAILEIGFISQRRDRIRLIDPRCQEAVGRAIAEAVAEYFGVPPTPLPG
                     SPPPEVILPVTAPRPASPPVFVCAGDPAGASDVAYARQLQARLDDHHGRAAFRVIQMK
                     GSACTVLECLRKWDEEVAPARPSHILLAFGRHDLEKDSSRDSRVPLARFEAALIEAVR
                     RTRALGAVPILATMAPIDDDHFHATRPREHYAADGGVQALRDRYNNAIRRVAWNTNAA
                     LVDLSLAAWQHEEEWINPAVSGDQLTPRGVERLVSLILREVPALIGAQPDGG"
BASE COUNT          462 a          960 c         1004 g          505 t
ORIGIN      
        1 gcgctgcgga ggatccggca tgcaactact tctcctgggt cgagcggctg caagatagcc
       61 ggcggacgca gtgggtgttg catcggactc cgatccgggt ttccgagatc ctgcgcacgg
      121 cgctctggga gaacgtcgat accgccgtcc tcacctcggc gactctggca accggaggct
      181 cgttcagcta cctgcgccgg tgcctgggcc tccccccagc ccgggagatc atcgtcggct
      241 cgcccttcga ctaccgccgc caggcgtgtc tctacatcgc ggcgcatctc gatcccccaa
      301 ccgaggggga gagctatcag aacgccgtgg cggctgagat cctgcgcatc ctggaactga
      361 cacggggccg tgccttcgtg ctgttcacct cctaccgtgc cctggagcgc gcgtatgctg
      421 tggttgcccc cgcggtgacc ttcccctgcc tgcgccaggg cgaggcatcc aacgcgcacc
      481 tgctccagga gttccggagc acccccaacg cttgcctttt tggcgtgcag agcttctggg
      541 aaggcgtgga tgtacccggg gatgcgctct cctgcgtgat catggaccgg ctcccctttg
      601 ccgtgccgga ccacccggtc caccgggcgc gggtccaggc gatcaaggag gagggcggcg
      661 actggttcaa cgactacgtc ttgccacagg cgcagatccg cctcaaacag gggttcggaa
      721 ggttggtgcg cacgcgcacc gatacgggaa tcgtctgtat cctggatagc cgcctggttc
      781 gaaaggcgta tgggagggcg tttctccact cgctgccacg gtgctccatc accaccgaca
      841 ttcgcgccgt ggcgcgcttt ctccaggagc gagatccatc ggggggagta caaaaaaaag
      901 gccgctagct gttcccgttc ctagcggcta ccctccacaa gggtccaacg gaaagtcacc
      961 acaagggaga gtgagaggct ttacgctgat cgaactacct ctatagacgc ctatttaagg
     1021 gaaaaagttc ccacattttg atgcccgctt ccaacaaagg cttcctccga tgcactagcc
     1081 tccgtctggc tgggcaccga tcagggcggg cacctctctc aggatcaggc tgaccaggcg
     1141 ttcgaccccc ctgggggtga gttggtcgcc ggagaccgcg gggttgatcc actcctcttc
     1201 atgttgccat gcggcgaggc tgagatcgac gagggcagcg ttggtgttcc aggcaacgcg
     1261 ccggatcgcg ttgttgtagc ggtcgcgcag ggcctgcacg ccaccatccg cggcgtagtg
     1321 ctcacggggg cgcgtggcat ggaagtgatc gtcatcgatg ggcgccatgg tggcgaggat
     1381 cggcaccgcg cccaaagccc tcgtgcggcg taccgcctcg atcagggccg cctcgaagcg
     1441 cgccaggggt accctgctgt ctcgcgagga atccttctcc aggtcatggc gcccaaaggc
     1501 caggagaata tggctggggc gggcgggggc cacctcctcg tcccacttgc gcaggcattc
     1561 gagcacggtg caggcgcttc ccttcatctg gatcacccgg aaagcggccc gcccgtggtg
     1621 atcatccaga cgtgcctgca gctggcgcgc gtaggccaca tcactcgcgc ctgccggatc
     1681 tcccgcgcac acgaagacgg ggggagaggc cggtcgcggg gccgtcacgg gcaggatcac
     1741 ctccggggga ggcgagcccg gaagcggggt gggcggaacg ccgaagtact cggcgacggc
     1801 ttcggcgatg gcccggccga ccgcttcctg gcagcgcgga tcgatgagcc ggatccggtc
     1861 acggcgctgg ctgatgaagc cgatctccag gatggccgag ggcatgtgcg cccagtggtt
     1921 ctccccatag cgcgcatccg tgcctaccgc cccatgcgag cgccaacctg ggtctacctt
     1981 ctcgcggatg gcgcgcacca catgcgcctc cagcaggcgg gccaggcggt ggctttcgcg
     2041 ctcgaagccg ttggttttgt cgtagagcgt cagcgtgccg gtgttcgggc caatgttgct
     2101 gtgcaggctg atcagcaggt cggcgcccag gtagttggcg tagagcgggc gcgcatcgat
     2161 gtcggaatcg aaatccacgg gcaacggctg gtggacccag tcgggcatgc cgatgtcgcg
     2221 caggtagtac ttggcggcct ccatccacct cggggcaccg ctgatccccg gttcgttcgc
     2281 cccctcgggg gcccgggtcg tgtggactgt ggcgcccagg cggcggagct cgcggcaggc
     2341 gtgcgtcgcg aaagcgatgt tcagctggtc ctccacttct ccctcgaaga agggccgctg
     2401 gtagcgccag cgcccgttac tctggcgcgt caggccatgc ccggggctga tcacgatcac
     2461 ttttccagag agtgggcccc gcggcaggga tgcttccgcc ggcgattgag cagcatggat
     2521 tggccctgcg ggcgcgcggt tcgccgccgc cgtgccgaga tgcaggaact gcgccgcgag
     2581 aacggcacgc acgggcgtgc cctggaccag gatgcgcgcg gcacggatct ccgggaagga
     2641 tgcgagcgtg gccgtcacct gcgccaccgc gacccggagc gcctcgggcg cgatggcgtg
     2701 cgacaggtcg accaccgtct ccgagccgcg ccgggtgagt ccgaggagac gcgttcccgg
     2761 gggaagggcg gttgtcagcg agcccttttg ctcttccacc gtcggccctg ccaggagcgc
     2821 atccagggcg tgtgccggcg tcgccgggcc tttgggtcgg taaaccgggc gcaggcgtgt
     2881 accctccagg aagaagattg gcagtgtacc cggcggaggc tcgacatgcg c
//
LOCUS       DAOSCV010000577         2926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_21903_length_2926_cov_3.031000, whole genome shotgun sequence.
ACCESSION   DAOSCV010000577 DAOSCV010000000
VERSION     DAOSCV010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_2926_cov_3.031000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLY56_13455"
     CDS             complement(<1..313)
                     /locus_tag="PLY56_13455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOJ22533.1"
                     /translation="MIQQCLNTLYVQSPEPHVRLDHDTLRVTIGDEEAVRVPLHHLGG
                     VVLFGGASMSQGGLERCAEEGREVTFLSLNGRFRFRVDGPTSGNVLLRLAQMDAHRDQ
                     AR"
     gene            complement(310..945)
                     /gene="cas4"
                     /locus_tag="PLY56_13460"
     CDS             complement(310..945)
                     /gene="cas4"
                     /locus_tag="PLY56_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOJ22534.1"
                     /translation="MAADDEGVMLSAIDHYAYCPRRCALIHVEQVWDENVFTLQGAAL
                     HERADEGFTTFEAGKRIERALPLWSEELGLIGKADTVEFLADGSVRPVEYKRGARAAR
                     AHDDLQLCAQALCLEEMLGVPVTTGAIFYHASRRTREVVFDDKLRAATREAIAQVRDL
                     LRTGRIPPPVNDARCRDCSLLHACQPQALAAAAGLDEDELFTPPSDEEMEE"
     gene            complement(979..>1645)
                     /locus_tag="PLY56_13465"
     CDS             complement(979..>1645)
                     /locus_tag="PLY56_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOJ22535.1"
                     /translation="TLTDPDVVAWSAGQVRGPVQLSFARSLDPVLPMEVAITRMAVAE
                     EVKGKKSSAEYAAWEAEQDEDKLRTMGRKSLIPYGLYVARGFISAHLASGTGFSEADL
                     ALFWEALLSMYDHDRSASKGMMSARGLYIFRHVGTDTNPQQCARQAILGCAPAHRLLD
                     LGTTSEDTEHIVSIYRLDATKPARCFADYAIAVHPDRLPAGVELYTASLEGGSVRLSR
                     LVG"
     assembly_gap    1646..1655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1656..2046)
                     /locus_tag="PLY56_13470"
     CDS             complement(<1656..2046)
                     /locus_tag="PLY56_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOJ22536.1"
                     /translation="MPEPIQNRYEFLYLFDCENGNPNGDPDAGNAPRIDPEDMHGLVS
                     DVALKRRIRNYVQIARENRMPNAIFVQHATNLNRQILRAHEETPGGYQEKVRTKSKVE
                     AARDWMCANFYDVRTFGAVMSTGPNAGQ"
     gene            complement(2039..>2926)
                     /locus_tag="PLY56_13475"
     CDS             complement(2039..>2926)
                     /locus_tag="PLY56_13475"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOJ22537.1"
                     /translation="PEEEREQEALQQQESSAAQRRAAELLDALRTGKRPDLLDSRYCA
                     LTLSGSGGRVMVREWMEGAFEELVSNVNAWFNDFSIVRRDGTGLTSPPKFLAVLASTA
                     RELNDVPAPHASVLWQAAVNGRPIPRQVMALALARVRIALIRDDPVSHAGMGLLKAYH
                     IRKGGMMTPYLNEEHPEPAYHCGRLMALLARVQYAALGDVGAGLVQRYYAAASATPAL
                     VLGRLIRTSQFHLNKLEPGLAYWHETRIAQVWGRIRDRVPAVLSLEEQSLFALGYYQQ
                     LAFDRSTSPTSNQKENNHA"
BASE COUNT          497 a          942 c          954 g          523 t
ORIGIN      
        1 tacgagcttg atcgcgatgg gcatccatct gcgccagacg gaggaggacg ttgccgctcg
       61 tcgggccatc cacccgaaat cggaagcgcc cgttcaggct gaggaaggtg acctcgcgtc
      121 cttcctcagc gcagcgttcg aggcccccct ggctcatcga ggcaccacca aagagaacca
      181 cgccccccag gtggtgtaga ggcacgcgca ccgcctcctc gtcgccgatg gtcacccgca
      241 gcgtgtcgtg gtcaagccgg acgtgaggct ctggcgactg cacgtagagc gtgttgagac
      301 attgctggat cactcttcca tctcctcgtc gctcggcggg gtgaagagct cgtcttcgtc
      361 cagcccggcc gcagcggcga gtgcctgagg ctggcaggca tgaaggagcg agcaatcgcg
      421 gcagcgcgcg tcgttcaccg gcggggggat gcgcccggtg cgcagcaggt cgcgtacctg
      481 ggcgatcgcc tcccgggtgg cagcgcgcag tttgtcgtca aagaccacct cgcgggtgcg
      541 ccgcgaggca tggtagaaga tggcgccggt ggtgaccggc acccccagca tctcctccag
      601 acagagcgcc tgggcgcaca gctggagatc gtcatgagcg cgcgcggcgc gcgcgccccg
      661 cttatactcc acggggcgca cgctgccgtc cgcgaggaac tccacggtat ccgccttgcc
      721 gatgaggccc agctcctcag accagagggg gagtgcgcgc tcaatgcgct tgcccgcctc
      781 aaacgtcgtg aagccctcat cggcgcgctc gtgcagcgct gccccctgga gggtgaagac
      841 gttctcgtcc cacacttgct ccacgtggat cagcgcgcag cgccgcggac agtaggcata
      901 gtgatcgatg gcggagagca tcactccctc atcatcagca gccatgtggt agaccctcta
      961 tgcctggcga gagcaccttc agccgacaag acgcgagagc ctgacgctcc ccccctcaag
     1021 agacgccgtg tacagctcga cgccggcagg aagccgatcc ggatgcacgg caatggcata
     1081 gtcagcgaag caccgcgccg gtttcgtcgc gtccagccgg tagatggata cgatgtgctc
     1141 ggtatcctcc gaggtcgttc ccaggtcgag caaccggtgg gctggcgcgc acccgaggat
     1201 cgcctggcga gcgcactgct gcggattggt atccgttccg acatgccgga agatgtagag
     1261 accgcgtgct gacatcatcc ctttgctggc cgaccggtcg tggtcgtaca tgctgaggag
     1321 ggcctcccag aagagcgcca ggtctgcctc ggagaaaccg gtgccgctcg cgaggtgcgc
     1381 gctgatgaag cctctcgcga cgtatagtcc gtaggggatc agtgacttgc ggcccatcgt
     1441 acgcagctta tcctcgtcct gctccgcctc ccacgcggca tactcggcgc tgctcttctt
     1501 ccccttcact tcctcagcca cggccatccg ggtgatagct acctccatcg ggagcacggg
     1561 atccagggag cgggcgaagg agagctgcac cgggccgcgg acctggccgg cggaccaggc
     1621 gacgacgtcg gggtcggtga gggtgnnnnn nnnnncctgg ccggcgttgg gcccggtgct
     1681 catcacggcg ccgaaggtgc gcacgtcgta gaaattggcg cacatccaat cgcgcgccgc
     1741 ttccaccttg cttttggtgc gcaccttctc ctgatatccg cccggcgtct cctcgtgggc
     1801 gcgcaggatc tgccggttca ggttggtggc atgctgcaca aagatggcat tgggcatccg
     1861 gttctcgcgc gcaatctgca cgtagttgcg gatgcgccgc ttgagcgcca cgtcggagac
     1921 caggccatgc atatcctccg ggtcgatccg gggggcattg ccggcatccg ggtcaccatt
     1981 cggattgccg ttctcgcagt cgaacaggta caggaactcg tagcggttct gaatgggttc
     2041 aggcatggtt gttctccttc tggttgctag tgggcgatgt gctgcggtcg aaagcgagct
     2101 gttggtaata gccgagagcg aagaggctct gctcttcgag cgagagcaca gcggggactc
     2161 gatccctgat gcgtccccac acctgggcga tcctcgtctc atgccagtat gcgagccccg
     2221 gttcgagctt gttcagatga aactggctgg tccggataag tcggcccagg acaagggcag
     2281 gagtggcgct cgccgcggcg tagtagcgtt gcaccaggcc cgctcccacg tcgccaagag
     2341 cagcgtactg gacgcgagcc agcagggcca tcagccggcc gcagtggtat gccggctctg
     2401 ggtgttcttc gttcaagtag ggtgtcatca tacctccttt gcgaatgtga tatgccttga
     2461 gcaagcccat ccccgcatgg ctgacgggat catcccggat caaagcgatc ctcacccgag
     2521 caagggcaag cgccatcacc tggcgcggga tgggccggcc gttgacagct gcctgccaca
     2581 gcacactcgc gtgtggggca ggaacgtcgt tcaactcgcg cgcagtactg gctagaaccg
     2641 ccaggaactt ggggggcgag gtgaggcccg taccgtcacg gcggacgatg gaaaagtcat
     2701 tgaaccacgc attgacgttc gataccagct cctcgaaagc gccttccatc cactcgcgta
     2761 ccatcacgcg ccctccgctc ccggagagag tgagcgcgca gtaccggctg tcgaggagat
     2821 cggggcgttt gcccgtgcgc agcgcgtcga ggagttccgc ggcgcgtcgc tgtgccgcag
     2881 aactctcctg ctgctggagc gcctcttgct cgcgctcctc ttcggg
//
LOCUS       DAOSCV010000578         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22079_length_2909_cov_3.777505, whole genome shotgun sequence.
ACCESSION   DAOSCV010000578 DAOSCV010000000
VERSION     DAOSCV010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22079_length_2909_cov_3.777505"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..1358)
                     /gene="dusB"
                     /locus_tag="PLY56_13480"
     CDS             complement(354..1358)
                     /gene="dusB"
                     /locus_tag="PLY56_13480"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOJ22538.1"
                     /translation="MMHPLRPLHVGPVVVDPPLILAPMAGFTHYPFRLLCREQGAGLV
                     CSEFVSSHGLVRRMKNTWVLVDLHDDERPVSVQIFGADPAVMADAARMIEDAGADIID
                     INMGCWVPKVSKTGAGAALLRSPETASAVMRAVAEAVRVPVTVKIRMGWTGQNLTGID
                     IARLAEDAGIRLITIHARPATRGYAGNADWDYIAQLKASVSIPVVGNGDVRSPEDALR
                     MFRHTGCDAVMIGRGALGNPWLFRRCLAFLRTGDPGPEPGVQERMETAARHVRLALAE
                     GPEDVVIREMRAHLAHYVKGFPGAAPLRERLMHLTRADEVLESLAAAATSLPAGTAGS
                     "
     gene            complement(1355..>2909)
                     /locus_tag="PLY56_13485"
     CDS             complement(1355..>2909)
                     /locus_tag="PLY56_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ22539.1"
                     /translation="VYSAYDEYDEARYVCQMIAAGCMGKGRSYRDHAVLYRMNSQSRA
                     LEDELRRMGIPYRVVGGLRFYDRKEVKDLIAYLRLVANPADSISLRRVINAPPRGIGA
                     TTMARLGDYAMSEGISLFEAMRRAGRAQGIQPAIRKRVADFVLLIDGLRELAATATVL
                     DTLKAIIARTGFEQTLQGETVIETDSRKANVQELLKVAEEFVGGGHGSALSAFLEEMA
                     LVSDVDNLREDEDGVALMTLHAAKGLEFPVIFMVGLEEGIFPHSRSMGDDAALEEERR
                     LCYVGITRAMQELYLLHACRRASWGTPQYNPPSRFLKDLPPSAIARDAESARFGQAGT
                     TVSLPPPPSPRVADRHDWEWDSPSASTQPGTPLDAEAILARYRPAEPAFRPGDKVQHA
                     TFGTGVVLSVEGEGDTAEVRVHFAGKGMKRLALAYAQLSKLGSGVGEPGGEDPAPEES
                     AAVDAPEATEVVLFARGDRVVHRRFGTGTVVSVHGEGPTAEVTVQFGHAGTRRLDPVI
                     SGLERVRPA"
BASE COUNT          496 a         1011 c          943 g          459 t
ORIGIN      
        1 tcgccgctag tatagcgatg atggctatga cgacgagcaa ttcgatcagg gaaaaccacg
       61 acgtttcatt catgctcctt ctagccggga ttgagccccg atagaccgct tcatcccggc
      121 agaccactgc cccgtgcagg gcatcttttc ccaaggcagc ccgataaccg tgccgccaga
      181 cagagcttac cagggctacg agcgcctgtc aagtattcat gcgcgattcc cggcaccgaa
      241 cctcggatga acggacacgg atggataggg gggcgaacac ccggggctcc caaggtgcgg
      301 aggattgcgc tcggccggtc ccgatcagcg cgcgccggca gccgggccgg cttctaggag
      361 ccggcggtcc ccgcggggag ggatgtggcg gccgcggcga ggctttcaag aacctcatcc
      421 gcgcgagtga ggtgcatcag gcgctcgcgc aggggcgctg cccccggaaa gcccttgacg
      481 tagtgagcga gatgcgcgcg catctcgcgg atgaccacat cctccgggcc ttcggcgagg
      541 gcgagacgca catgccgcgc cgccgtctcc atgcgctcct gaacgcccgg ctcgggcccc
      601 gggtcgccgg tacgcaggaa ggcgaggcaa cgccggaaga gccacgggtt gcccaacgca
      661 ccgcggccaa tcatgacggc atcgcatccg gtatgtcgga acatacgtag cgcatcctcg
      721 ggcgagcgga catcgccatt gcccacgacc ggaatcgaca cggatgcctt cagttgggcg
      781 atgtagtccc aatcggcgtt gcccgcgtag ccacgggtgg ccgggcgcgc gtggatggtg
      841 atcagacgga tccccgcgtc ttccgccagg cgggcaatat caatcccggt gaggttctgg
      901 cctgtccacc ccatccggat cttcacggtc accggcacgc gaacggcctc ggccacggcg
      961 cgcatgacgg cgctggccgt ctcaggagag cgcaggagcg ctgcacctgc gcctgtcttg
     1021 gagaccttgg gcacccagca ccccatgttg atatcgatga tatccgcacc cgcgtcctcg
     1081 atcatgcgcg cggcatccgc cattaccgcg ggatccgcgc cgaagatctg caccgacacg
     1141 ggacgctcgt catcgtgcag gtcgaccagc acccaggtgt tcttcatccg gcggaccagg
     1201 ccgtggctgc tgacgaactc tgagcagaca agccccgcgc cctgctcgcg acagagaagc
     1261 cggaacggat agtgggtgaa tccggccatc ggggcgagaa tcaggggggg atcgaccacg
     1321 accgggccga catggagggg tcggagcgga tgcatcatgc cgggcggacc ctctccagtc
     1381 cagagatcac cggatccagg cgcctggtgc cagcatggcc gaactgcacg gttacctccg
     1441 ccgtcggacc ctcgccatgc acggagacca cggtgccggt gccgaaacgg cggtgtacca
     1501 cccggtcgcc ccgcgcgaag aggaccacct ccgtggcctc gggcgcatcg accgccgcgc
     1561 tctcttctgg cgccggatcc tcacccccgg gttcgccgac cccactcccc agcttcgaga
     1621 gctgcgcgta ggcaagcgcc agccgcttca tccccttgcc ggcaaagtgc actctcacct
     1681 ccgcggtgtc gccctcgcct tcgacgctga ggaccacgcc cgtgccaaag gtggcgtgct
     1741 gcaccttgtc tcctggccgg aaagccggct ctgccggccg gtagcgcgct aggatcgcct
     1801 ccgcgtcgag gggcgtgccc ggctgtgtgc tggccgaagg cgaatcccat tcccaatcgt
     1861 gacggtctgc cacgcgaggg ctcgggggcg ggggcaagga aacggtcgtg ccagcctggc
     1921 caaagcgggc gctctccgcg tctcgcgcga ttgccgatgg gggcaggtct ttcaggaatc
     1981 gggaaggcgg gttgtattgg ggcgtgcccc aggaggcacg ccggcaggcg tgcagcaggt
     2041 acagctcctg catggcgcgc gtgatgccca cgtagcagag ccggcgctcc tcttccagcg
     2101 cggcatcgtc gcccatcgag cggctatgcg ggaagatccc ctcctccagg ccgaccatga
     2161 agatcaccgg gaactccagg cccttagcgg cgtgcaacgt catcagcgcc acgccatcct
     2221 catcctcgcg caggttatcg acatcagaga cgagcgccat ctcctccagg aacgcgctga
     2281 gggccgagcc gtggccccct ccaacgaact cctccgccac cttcagcagc tcctgaacgt
     2341 tcgccttccg tgaatcggtc tcaatcacgg tctcgccctg cagcgtctgc tcgaagcccg
     2401 tgcgcgcgat gatcgccttc agggtgtcga gaacggtcgc ggtggcggcc agctcgcgaa
     2461 ggccgtcgat gaggaggacg aagtcggcga ctcgcttgcg gatcgccggc tgtatcccct
     2521 gggcgcggcc ggcgcggcgc atcgcctcga agagcgagat tccctcgctc atggcgtagt
     2581 cgcccagccg cgccatcgtc gtggcgccaa tcccgcgcgg cggagcgttg atcacgcggc
     2641 gcagcgagat ggagtcggcc gggttggcga ccaggcgcag gtaggcaatc agatccttca
     2701 cctctttgcg gtcgtagaac cggagcccgc ccaccacgcg ataggggatc cccatcctgc
     2761 gcagctcgtc ctccagcgcg cgcgattgcg agttcatccg gtagagcacc gcgtggtcac
     2821 ggtagctccg ccccttcccc atgcatcctg ccgcgatcat ctggcagacg tagcgcgcct
     2881 cgtcatactc gtcataggcc gagtaaacc
//
LOCUS       DAOSCV010000579         2909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22081_length_2909_cov_3.119131, whole genome shotgun sequence.
ACCESSION   DAOSCV010000579 DAOSCV010000000
VERSION     DAOSCV010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22081_length_2909_cov_3.119131"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1638)
                     /locus_tag="PLY56_13490"
     CDS             complement(184..1638)
                     /locus_tag="PLY56_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22540.1"
                     /translation="MTPSWRTHRSVRFWLTAWNVSIVAVVILTLGIASHYVLQGDLIR
                     SLDRQMLDRGMGIVRALEQAPADEEKRKAEWQRREAEERARPTRPFRQPGRDGERNRS
                     GSATLHRPRALTLAYLDYMRLTPEAPYDEASFRRAAETGEIVRVTTTLEGDPVRILSL
                     PVRHDGRVDGVVQIVRILSDYQDALRASSRTLLMLTPFALLLSGFVGAFLTGRALHPV
                     RTLRHAADQIQEESLSQRLPVIGGDEFAGLAETFNAMLDRLERAFRQQARFTADASHE
                     LRTPLTVLRGNISLALARSRTAEEYRETLERVHQATERMSGLVEDLLLLARADASQLL
                     TDVEEVPLSEILTAAAEAIHDRAIAVELPSDEANLCVNGNHRLLVTLFTNLLTNAHRH
                     TPPEGNVGIRAIRRRDSVAITVHDTGEGIPPEHLPHILDRFYRVDDSRSSKSGGAGLG
                     LAICQSIARAHGGTLEIQSTPGKGTAATVTIPLA"
     gene            complement(1635..2360)
                     /locus_tag="PLY56_13495"
     CDS             complement(1635..2360)
                     /locus_tag="PLY56_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22541.1"
                     /translation="MRVLLIEDEAAIAEFVQRGLQEYHYHVTVAADGETGLRLALEER
                     FGVIILDLMLPRRDGLSVCRELRERRVTTPILMLTARDAVRDRVRGLETGADDYLCKP
                     FDFSELLARVQALARRDRIHKGRTIRVADLEVDTTARTVTRGGRRMDLTAREYELLEA
                     LAAHEGQILSREVIQERVWMDDESYSNTVDVHIKGLRKKIDADFPVKLIHTVYGQGYV
                     LEVRDPAPKAETPQASTSRGTSR"
     gene            complement(2457..>2909)
                     /locus_tag="PLY56_13500"
     CDS             complement(2457..>2909)
                     /locus_tag="PLY56_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22542.1"
                     /translation="DARSAQGTWNGRPLAATAEWALIRTRGNALLGATLVQGQLSWGD
                     LKLESEPRGPHRLLAVDRAGRSLLVEGRFHPEAGSVITLDHAGKRISPYTVVSATEAG
                     ANTRVVVAEEPGFEWNAGNQTATFLFAPGGKFQGEHVVGQIPVARATR"
BASE COUNT          441 a          963 c         1020 g          485 t
ORIGIN      
        1 cccaacccag cccggcaggg cttcgctcgc aagcagcgcg gtgatttatc gccgcacgcc
       61 gggttgcgcg ctgcctccca ggcttcggat tccttatgtc ggaatctcag gaagccccga
      121 cacgtgatcg ccggggcgat gcgttgacgg agatggcctg cccgcccggg cgcggccgga
      181 gtgtcacgcg agggggatgg tgactgtcgc ggcggtgcct ttgccgggcg tgctctggat
      241 ctcgagcgtg ccgccgtggg cacgggcgat gctctggcag atcgcgaggc cgagaccggc
      301 gcccccggac ttgctggagc gcgagtcatc cacccggtag aagcggtcga ggatgtgcgg
      361 gaggtgctcc ggcgggatcc cctcgccggt gtcatgtacg gtgatggcga cggagtcgcg
      421 acgccggatg gcgcggatcc ccacattgcc ctcgggcggc gtgtgccggt gggcattcgt
      481 caacaggttc gtaaagaggg tgacgagaag gcggtgattc ccattcacac acaggttggc
      541 ctcgtccgat gggagctcaa cggcgatcgc acggtcgtgg atcgcctcgg ctgccgcggt
      601 cagtatttca gaaagcggaa cttcttccac gtcggtgaga agctgcgacg catccgcacg
      661 cgcgaggagc aggaggtcct cgacgaggcc gctcatccgc tccgtggcct gatgtacgcg
      721 ctcgagtgtc tcccgatact cctcggcggt gcgcgaccgg gcgagggcga ggctgatgtt
      781 gccccggagc acggtgagcg gagtgcgcag ctcgtgcgag gcatccgccg tgaaccgcgc
      841 ctgctggcgg aatgcccgct ccaggcggtc tagcatcgcg ttgaacgtct ccgcgagtcc
      901 ggcgaactca tccccaccga tcaccgggag ccgctgcgac aggctttcct cctggatctg
      961 atcggcggcg tggcgcaggg tgcgcaccgg atggagggca cgcccggtga ggaaggcgcc
     1021 gacgaaaccc gagaggagca gcgcgaatgg cgtgagcatc aagagcgtgc ggctgctggc
     1081 gcgcagggcg tcctggtagt cgctcaggat gcgcacgatc tggacgacgc catcgacgcg
     1141 cccgtcgtgg cgcaccggga gcgagaggat ccgcaccgga tcgccttcga gtgtggtggt
     1201 gacgcgcaca atctcgcccg tctcggcggc acggcggaag gaagcctcgt catacggcgc
     1261 ctccggggtc aaccgcatgt aatccaggta cgcgagcgtg agcgcccggg ggcggtgaag
     1321 cgtggcgctc ccggaacggt ttctctcgcc gtctcgccct ggctggcgga aggggcgcgt
     1381 gggccgggcg cgctcctctg cctcgcgccg ctgccattcc gcctttcgct tctcttcatc
     1441 tgccggtgct tgctccaggg cacgcacgat tcccatgccc cggtcgagca tctgccggtc
     1501 gagcgagcga atcaggtcgc cctgcaggac gtaatgcgac gcgatgccca gcgtcaggat
     1561 aacgaccgcg acgatgctga cgttccaggc cgtgagccag aaacgaaccg aacggtgagt
     1621 gcgccaggag ggggtcatcg cgatgtgccc cgagaggtgc tggcctgggg cgtctccgct
     1681 ttgggcgccg ggtcgcgcac ctcaagcacg tagccctggc catatacggt atggatcagc
     1741 ttcacgggga aatcggcgtc gatcttcttg cgcaggccct tgatgtgcac gtccaccgtg
     1801 ttcgagtagc tttcgtcatc catccagacg cgctcctgga tcacctcgcg gctcaggatc
     1861 tggccttcat gggcggcgag tgcctccagc agctcatact ctctcgcggt gagatccatc
     1921 ctgcgcccgc cgcgggtgac ggtgcgcgcc gtcgtatcca cctccagatc ggccacgcgg
     1981 atggtccggc ccttgtggat ccggtcgcgc cgggccaggg cctgcacgcg ggccagcaac
     2041 tcgctgaagt cgaacggctt gcacaggtag tcgtcggcgc cggtttccag gccgcgcacg
     2101 cggtcgcgca cggcgtcgcg cgcggtgagc atcaggatcg gcgtggttac ccggcgctcg
     2161 cgcagctcgc ggcaaacgct cagcccgtcg cgacggggca gcatgaggtc caggatgatc
     2221 acgccgaagc gttcttccag ggccaggcgc aacccggtct cgccgtcagc ggcgacggtg
     2281 acgtgatagt ggtattcctg caagccacgc tgcacgaact ccgcgatggc cgcctcatct
     2341 tcgatcagta gtacccgcat tcctctcctc agatgatggc aacggggcgc atcgtccgga
     2401 acataggacg ccgagacgcg ccagcgggtt gcatggagcg ggccggtctg ccatgatcac
     2461 cgggtcgcgc gggccaccgg gatctgcccg accacgtgct cgccctggaa cttgcccccg
     2521 ggggcgaaga ggaacgtcgc ggtctggttt cctgcgttcc attcgaagcc cggctcctcg
     2581 gccactacca cccgggtgtt cgcgcctgct tccgtggccg aaacgaccgt gtagggcgag
     2641 atccgctttc cggcgtggtc gagcgtgatc acgctcccgg cctcggggtg gaaccgaccc
     2701 tcgaccaaga gcgagcgccc cgcgcggtcg accgcgagca ggcggtgggg gccccgaggc
     2761 tccgattcca gtttgagatc gccccaagag agctggccct gaacgagcgt cgcgcccagg
     2821 agcgcgttcc cgcgcgtccg gatcagcgcc cattccgcag tggccgcgag tggccggccg
     2881 ttccaggtgc cctgtgcgct gcgggcatc
//
LOCUS       DAOSCV010000580         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22090_length_2908_cov_3.235542, whole genome shotgun sequence.
ACCESSION   DAOSCV010000580 DAOSCV010000000
VERSION     DAOSCV010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22090_length_2908_cov_3.235542"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1004)
                     /locus_tag="PLY56_13505"
     CDS             complement(126..1004)
                     /locus_tag="PLY56_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOJ22543.1"
                     /translation="MPTVVFVSFREPGKILPFGPGSLDLRVGSYVVAETARGVELGVV
                     RRERQEVPLKELTQPLRTIIREATPEDHAQARANLQREQEALGICAAKIAKHSLPMRL
                     LRAEYTLDRARIVFFFYAEGRVDFRELVKDLASTFRTRIELHQVGARDASKLVGGYGR
                     CGRELCCAAWLSGFEPISMKMAKDQDLALNPTKFSGQCGKLMCCLRYEVDLYRQAKQG
                     LPRVGATIATPQGNARVVEINIPAGRIQVEYPETGVRVGLALRDVVTSCGSECASGEG
                     CAGCAGNSPAAPGSHH"
     gene            1468..2322
                     /locus_tag="PLY56_13510"
     CDS             1468..2322
                     /locus_tag="PLY56_13510"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22544.1"
                     /translation="MEEIDVAEDGLLSGTFVYTMDPKGRVIMPRRFRERLGVPFVLTK
                     GIGGCLLAMSRERWQPLVDRYSSESIIFQRFYLAGAVECRPSPKSGRFLIPHPLREFA
                     EILPAQEATVAGIGVAVEIWNKQRWEHASKHPEQRDRRQLRFELDLEPVATEPFAMRV
                     RSLLGIPIVETKGTMDVRAGRLLAARMQEVTPGSVPAVVLDLRQATRLRTTPAGLRWA
                     LAQQRARGVAVCLLTSDPAASRLLSRYADVFATLEDALWWLEEHTAGASAAEEMPPPE
                     APPSLGVE"
     gene            complement(2497..>2908)
                     /locus_tag="PLY56_13515"
     CDS             complement(2497..>2908)
                     /locus_tag="PLY56_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22545.1"
                     /translation="VYREVKLLPPHRAERVQASIPYAPEVPPPLAEAELLRYLGLPGI
                     RPRYVPVGYEYEAFSHTRCGCPCGEPVAHIQYSDGLRALSVFASRHLPAEAEPVAVML
                     PVGDMLRLRHRGVAFAFLGEVGLPELHRMARSLP"
BASE COUNT          488 a          989 c          948 g          483 t
ORIGIN      
        1 gcgcgttcag acccccgccg ggcgcgagct cgccagcgag gtcctcgcga cgcccgccga
       61 tgactggtgg ttcggcaaca agcacgaccc gcactgggtc accgggaaag gctgaggcgt
      121 gcgcttcagt gatgcgagcc gggggctgcc ggtgagttcc ccgcgcaccc ggcgcatccc
      181 tctccggaag cacattcact gccgcaagaa gtcaccacat ccctgagcgc caaccccacg
      241 cggacgcctg tctccggata ctccacctgg atacggcctg ccgggatgtt gatctcgacg
      301 acgcgcgcgt taccttgggg ggtggcgatg gtagctccaa cccgagggag gccttgcttt
      361 gcctgccggt agagatctac ctcataccgc aggcagcaca tcagcttgcc acactggcca
      421 gagaatttcg tcgggttgag ggccaggtcc tgatccttgg ccatcttcat ggagatcggc
      481 tcgaagccac tcagccaggc ggcgcagcag agctcgcgcc cgcaccgccc gtagccaccg
      541 acgagcttgg aagcatcacg cgcgcccacc tggtgcagct cgatgcgcgt ccggaaggta
      601 ctcgcgagat ccttcaccaa ttcgcggaaa tcgactcgcc cctccgcata gaagaagaag
      661 acgatccggg cccggtcgag ggtgtactcg gcgcggagga gccgcatcgg gaggctatgc
      721 ttggcgatct tggcggcaca gatgcccaac gcctcttgct ctcgctggag gtttgcacgt
      781 gcttgagcat ggtcttccgg cgtcgcctcg cggatgatgg tacgcagagg ctgcgtcaac
      841 tccttgagcg gcacttcctg gcgctcgcga cgcaccacgc ccagctccac gccccgagcc
      901 gtctctgcga cgacgtagct gccgacacgc agatcgagcg atccgggccc gaacgggagt
      961 atcttccccg gttcacggaa gctgacgaag acgacggttg gcatacttca agtccttcca
     1021 gagggtatcc gggtctatct ggtctccatc ataccatacg cctggatggg gatcaaccgc
     1081 gctggcgcac tccaaccagg tcgaaggcag gcgcacgcgg atcggtggtg aacggatgaa
     1141 cgccgcccca tccctgccgg cgcggtgttt gcggctccgc tcttccgcag gtcctccgcg
     1201 ggcgcaggcg atggaaaagc tgctctccac acgggttggg ggtgaagcca tgccgggcag
     1261 gcggccgaaa aagtggcgcg cgggtcaggc aggatccccg gcgcatgagg gagaatagga
     1321 gaggggagag cgtccagcaa ccctcttcgc tggcgcgcta tggggccgct acgcctgacg
     1381 ccttcgccgg caccggtgcg ttccgcgccg ctgtgtgcca cccatccccg gagttgttgt
     1441 atcggttgtt ggcacagggg ggattccttg gaagagattg atgtggctga ggatggtctt
     1501 ctgtcgggca cgtttgtcta cacgatggat ccgaagggcc gtgtgatcat gccccggcgc
     1561 ttccgagagc gccttggcgt gccctttgtc ctgaccaagg ggattggcgg atgcctgctg
     1621 gccatgtcgc gcgagcgttg gcagcctctg gtggaccgct actccagcga gagcatcatc
     1681 ttccagcgtt tctacctcgc cggcgcggtg gagtgtcggc ccagccccaa gtccggccgc
     1741 ttcctcattc cccacccgct ccgggaattc gctgagattc tgcccgccca ggaggcgacc
     1801 gttgccggca tcggcgtggc ggtggagatc tggaataaac agcgctggga gcatgcgagc
     1861 aagcatcctg agcagcgcga ccgccgccag cttcgtttcg agctggacct ggagccggtt
     1921 gccaccgagc ctttcgcgat gcgcgtgcgc tccctcctcg ggatcccgat tgttgagacg
     1981 aagggcacca tggatgttcg cgcaggtcgc ttgctggccg cgcgcatgca ggaagtgact
     2041 cccggctccg tgcccgccgt tgtcctcgac ctgcgccagg caacgcgcct ccgcaccact
     2101 ccggctggat tgcgctgggc cctcgcccaa cagcgcgcgc gcggcgtcgc cgtctgcctg
     2161 ctcaccagcg acccggcggc aagccgcctc ctctcgcgct acgccgatgt ctttgccacc
     2221 ctcgaagacg cgctgtggtg gctggaagag cacaccgccg gcgcatcggc ggccgaggag
     2281 atgcctccgc ccgaggcacc tccctcgctt ggcgtcgagt agccctctcg ggaagccggg
     2341 atctggagat gcgggcgatc ctgccacggt cccttcggcg tcctccgcga tcccgccgtg
     2401 gatggccatg cctccgcatg tcccgcacaa cccccgggca catacccggc ccgtccgtac
     2461 ttatctcgga atcccaacac gggaagccct ggggggctat gggagcgacc gggccatgcg
     2521 gtgcagctcg gggaggccaa cctcgccaag gaaggcgaac gccacgccac gatggcgcag
     2581 gcgaagcata tctcccacgg gcagcatcac ggccaccggt tccgcctccg cgggcaggtg
     2641 cctgctggca aagacggaaa gcgcgcgcag cccatccgaa tactggatgt gcgcgaccgg
     2701 ctcaccacaa ggacagccac accgcgtgtg cgagaacgct tcgtactcat accccaccgg
     2761 cacgtagcgg ggccggatgc cgggcaagcc cagataccgc aacagctcgg cctcggccag
     2821 gggagggggg acctccgggg cgtaggggat ggacgcctga acgcgctctg cgcgatgcgg
     2881 cggcaacagc ttaacctcgc gatagacc
//
LOCUS       DAOSCV010000581         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22092_length_2908_cov_3.152121, whole genome shotgun sequence.
ACCESSION   DAOSCV010000581 DAOSCV010000000
VERSION     DAOSCV010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22092_length_2908_cov_3.152121"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="PLY56_13520"
     CDS             complement(<1..98)
                     /locus_tag="PLY56_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006874256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 family protein"
                     /protein_id="HOJ22546.1"
                     /translation="MVGFDNEKYLREQTQAIQERIAKFGGKLYLEFG"
     gene            complement(421..>2908)
                     /locus_tag="PLY56_13525"
     CDS             complement(421..>2908)
                     /locus_tag="PLY56_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOJ22547.1"
                     /translation="ESTNRLLTGEGPVSARQVLAPALALPARASAHQVSAIGHSHIDV
                     AWLWPLRETIRKCSRTFSTVLRYMERYPDYKFGQSQPQLYAFTKEHYPKLYARIKEAV
                     AAGRWEPVGAMWVEADCNLSSGESLVRQVVHGKRFFMDEFGFETDVLYLPDVFGYSAA
                     LPQILRKARVPYFMTQKISWNQFNQFPHHTFLWQGIDGTRVFSHFLPANSYNSVYTAE
                     ELRRAEANFKEADRARSWLYLFGYGDGGGGPTKEMLEAAERVKNLDGVPRVEQEFARD
                     FFRRAEAEAKDLPVWAGELYLELHRGTYTTQAHNKRMNRRCELLLRDAEFFASIRPNA
                     MPSYPAETLDRCWKLVLLNQFHDVLPGSSIGWVYEDSREQYAEVEREGERIVASALAS
                     LCERIDTAGCRQPVVVWNSLSRAHRGAVMLPLQGSAPRSVRDPRGVRHPVQETTVGSE
                     RYLLFIAEAPEHGYALYDLSEEAPDAGYERVHVTERSLENELLRIELDEHGMIARVFD
                     KRRGREVLAPGAKANVLQLFDDRPNNWDAWDIDAFYAETGRDVTELSEIRVVESGPVR
                     GAIEIVRRFGDSEVRQRVQLLAGSGRVDFVTEAEWHEAHKLLKVAFPVDLNASRATFE
                     IQYGHVERPTHRNTSWDLARFEVVAHKWVDLSEGDYGVALLNDCKYGHDVLGNTLRLS
                     LLRAPKNPDPNADMGHHRFTYSLYPHAGDLRHGEVVAEGYRLNVPLRAVPVKPRSGEL
                     PAECSFFTVDNPNVFVEVVKRAEREDAWVVRLYEAHNTRGSVSLSTRLPVEHAYLADL
                     MEDPIEEVPVSDGTIRIDIKPFEIVTLLLR"
BASE COUNT          496 a          988 c          923 g          501 t
ORIGIN      
        1 ccgaactcca ggtagagttt gccgccgaac ttcgcgatgc gctcctggat tgcctgggtc
       61 tgctcgcgca agtacttctc gttatcgaat cctaccattc tcgccgtgcc ggccatactt
      121 tggttttcta actccttgct agattccggc actttgactc gcaggaaaga gggcctgggg
      181 tgccgtcact tctcattata tccgaggcga aagcggattg caacagaacg aaacaacggc
      241 aaaggtcagg ctccggaagc atacggtcat gctgcacgag ggcaccgccc taccccacgc
      301 cggcatcgat accgtggatg aacagacgcg gatgggagag tgcgaagata cccgtgggga
      361 gggagcgcgg gccgacctga cggactgaat ctgcccgcgc tcccctgaat cctcctcaat
      421 tcaacgaagg aggagcgtta cgatctcaaa cggtttgata tcgatccgga tcgtgccgtc
      481 gctcaccggc acctcctcga tgggatcttc catgagatcc gccaggtagg catgctctac
      541 cggcagcctc gtggagagcg acaccgagcc ccgagtgttg tgcgcttcgt agaggcgcac
      601 cacccacgca tcctccctct ccgcacgctt caccacctcc acgaagacgt tggggttatc
      661 gacggtgaag aaggagcact ccgcgggaag ctcgcccgaa cgcggcttca ccggcaccgc
      721 gcgcagcggc acgttcaggc ggtagccctc ggccaccact tcgccgtggc gcaagtcgcc
      781 cgcgtgggga tagagcgagt aggtgaagcg gtgatgcccc atgtcggcgt tcgggtccgg
      841 attctttggc gcgcgcagca ggctgaggcg cagcgtgttg cccagcacgt cgtggccgta
      901 cttgcagtcg ttcagcaggg cgacgccgta atctccctcg ctcagatcca cccacttgtg
      961 cgccaccacc tcgaagcgcg ccagatccca cgacgtattc cggtgcgtgg gccgctccac
     1021 gtgcccgtac tggatctcga aagtcgcccg gctggcgttc aggtccaccg ggaacgccac
     1081 cttgaggagc ttgtgcgcct cgtgccactc cgcctccgtc acgaaatcga cgcgcccgct
     1141 ccccgcgagg agctggaccc gctgacggac ctcggaatcg ccaaagcggc gcacgatctc
     1201 gatggcgccg cgcaccggac cggactccac cacgcggatc tcgctgagct cggtcacgtc
     1261 gcgtcccgtc tcggcataga aggcatcgat gtcccaagcg tcccagttgt tcggccggtc
     1321 gtcgaagagc tggagcacgt tcgccttcgc ccccggcgcg agcacctccc gcccccggcg
     1381 cttgtcgaaa acgcgggcga tcatgccgtg ctcgtccagc tcgatgcgca ggagctcatt
     1441 ctccagcgag cgctcggtca catgcacccg ctcgtaaccc gcgtccggcg cctcctccga
     1501 gaggtcatag agcgcgtagc cgtgttccgg ggcttcggcg atgaagagga gataccgctc
     1561 gctcccgacc gtcgtctcct gcaccggatg gcggacgccg cgcgggtctc tcaccgagcg
     1621 gggcgccgaa ccctggagcg gcagcatcac cgcgccacgg tgcgcccggc tcaggctgtt
     1681 ccagacgacc accggctggc ggcagcccgc cgtatcgatc cgctcgcaca ggctggccaa
     1741 cgcgctcgca acaatcctct cgccctcgcg ctcgacctcg gcatactgct cgcgcgagtc
     1801 ctcgtacacc cagccgatgg atgaccccgg cagcacatcg tggaactggt tgagaagcac
     1861 cagcttccag caccggtcga gcgtctccgc ggggtagctc ggcatcgcgt ttggtcggat
     1921 gctcgcgaag aactcggcgt cgcggaggag caactcgcag cgccggttca tgcgcttgtt
     1981 gtgcgcctgc gtcgtgtagg tgccgcgatg gagctccaga tagagctcgc cagcccagac
     2041 gggcaggtcc ttcgcctcgg cctcggcgcg gcggaagaag tcgcgcgcga actcctgctc
     2101 cacccggggc acgccatcca ggtttttgac gcgctccgcc gcctcgagca tctccttggt
     2161 cgggccgcct ccgccatcgc cgtagccaaa gaggtagagc caagaccggg cgcggtcggc
     2221 ctccttgaag ttggcttctg cccggcgcag ctcctctgcg gtgtagacgc tgttgtagct
     2281 gttcgccggc aggaagtgag agaagacccg ggtgccgtcg atcccctgcc acaggaacgt
     2341 gtggtgcggg aactggttga actggttcca ggagatcttc tgcgtcatga agtagggaac
     2401 gcgcgccttc cgcaggatct gcggcagcgc cgcggaatag ccaaagacgt ccggcaggta
     2461 gagcacgtcg gtctcgaagc cgaactcatc catgaagaag cgcttcccat gcaccacctg
     2521 gcggaccagg ctctcgccgg aggagaggtt gcagtcggcc tcgacccaca tcgcgcccac
     2581 gggctcccag cgcccggcgg ccacggcttc tttaatgcgc gcgtacagct tcggatagtg
     2641 ctccttggtg aaggcgtaga gctgcggctg gctctgcccg aacttgtagt ccgggtagcg
     2701 ctccatgtag cgcagcacgg tggagaaggt gcgcgagcac ttgcggatcg tctcgcgcag
     2761 cggccacagc cacgccacgt cgatgtgcga gtggccgatg gcgctcacct gatgcgccga
     2821 ggcgcgcgcc ggcagcgcca gggcgggcgc tagcacctgg cgggcggaaa cgggaccctc
     2881 gccggtgagg agccggttgg tgctctcg
//
LOCUS       DAOSCV010000582         2906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22109_length_2906_cov_5.509996, whole genome shotgun sequence.
ACCESSION   DAOSCV010000582 DAOSCV010000000
VERSION     DAOSCV010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22109_length_2906_cov_5.509996"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            850..1887
                     /locus_tag="PLY56_13530"
     CDS             850..1887
                     /locus_tag="PLY56_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22548.1"
                     /translation="MKKIGVGIIGSGGIAQAVHIPGYQKLENVEVLAVADANPAVAEA
                     AATKFNIPHRFTDYLQMLEMPEIQVVSVCTPNYLHRDATIAACKAGKHVLCEKPIAMN
                     AKEGREMVEAARANKVKFTVGYNQRCTKEAIAIKKFVDDGLLGEVYYARAQALRRRGI
                     PGWGVFTQKDKQGGGPLIDIGVHILDLTLYLMGHPKPVSVSGMACAKFGPREGVLGLM
                     GQWDPKTFTVEDFAVGLVKFENGAALCLESSFCANIEKDVMDTTLLGTNGGASVFPPT
                     IMREESGALTVSHITSLRDIQSHGENIRRFIQAIVDDTEPLVTGEQALMVAEIIDAIY
                     ESQETGKEVRL"
     gene            1991..>2906
                     /locus_tag="PLY56_13535"
     CDS             1991..>2906
                     /locus_tag="PLY56_13535"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ22549.1"
                     /translation="MRCCAFLLAMCVVFAPATRAESVSQTEMLLTETFETVADGALPE
                     GWQPAGGNWSVTQGRLRGEATVYLSKILLPTPAQSDLAFEADVAFEKAADAGRWVALA
                     VRNPEGAGAPHLQFTHRFDRRQPNGLEFGWLASHEPVKWSVLSLGSADIAPELGKAHR
                     LRFEVRGWQARGFIDDELVLEQPLPLSTLVAGRLALIISGATATFDNIRVWKLPALGA
                     EEFDRLLEQTCTTPLVIAHRGNSLHAPENTLEAIRQAIEAGADLVEVDVNMSKDGVVV
                     LMHDYTVDRTTNGKGKVSDLTLAELKALD"
BASE COUNT          532 a          956 c          910 g          508 t
ORIGIN      
        1 atggcgaggt aggctttgat gaagacctcc tgcgtcagat cctcggcatc ggccgtggag
       61 ccggtgtacc gagcgacgta gttgaggatc ttgttgcggt agcggtccac cagcaactca
      121 tatgcctcga tatcatcctg cttggagcgt tccaccagca ggagatcggc cacttccacc
      181 aggaactccc ttcgggccgc gctgccgggc gccggccagt agcattgacg gatgttccgg
      241 agcgaaggtt cagaggatcg ctccggaact tgggaggatg acgcacccga tacgaccgcg
      301 gcggcacccc gccggcaggc ggcggccctc atcggtgagg gcgccggccc gcgctttgcc
      361 ggggctatcg cgcccgccgc agaccaggca gtagctaccg gtgtctctat gataccatcg
      421 gcgctggcca cggtcaacct tccgccagca cgctctggga ttccgagata aggagaaggc
      481 tgcccgcacg cgcggagctc gaagctccgc gctgctttcc ggcaaagccc cttcgggctt
      541 ctcctcccag ccagcgccgt tttgagacat ctgggagtat ccccaaccca gcccggcagg
      601 gcttcgctcg caagcagcgc ggtgatttat cgccgcgcgc cgggttgcgc cctgccttcg
      661 aggcttcgga ttccttatat cggaatccca gcagcacgct caagatcgtc gccgtgcaat
      721 cgcttgacgc ccttctgccc gagtgctaca atggcctcgg tagtacacct gctctcccga
      781 gcaatcaact acgcggcaga tccggcggca ccccgcccaa cccgcgccag aatccagaag
      841 gagcgttcca tgaagaagat cggtgtcggc atcatcggca gcggagggat tgcccaagcg
      901 gtgcacatcc ccggctacca gaaactggaa aacgttgagg ttctagccgt cgcagatgcc
      961 aacccggccg tggccgaagc cgccgcgacc aagttcaaca tcccccaccg gtttaccgac
     1021 tacctccaga tgctcgagat gccggagatc caggtggtga gcgtttgcac gcccaactat
     1081 ctgcaccgtg acgccaccat cgcagcgtgt aaggccggca agcacgtgct gtgcgagaag
     1141 ccgatcgcca tgaatgccaa agaaggccgg gaaatggtgg aggccgcccg cgccaacaag
     1201 gtgaagttca ccgtgggcta caaccagcgc tgcaccaagg aggcgatcgc gatcaagaag
     1261 ttcgtagatg atggcctctt gggcgaggtc tactatgccc gcgcgcaggc gctgcggcgc
     1321 cgtggcatcc cgggctgggg cgtcttcacc caaaaggata agcagggggg cggcccgctc
     1381 atcgatatcg gcgtccatat cctcgacctg acgctctacc tgatgggcca cccgaagccc
     1441 gtctcggtct ccgggatggc ctgcgccaag ttcggcccgc gcgagggcgt gctcgggctg
     1501 atgggccagt gggatccgaa gaccttcacc gtcgaagact ttgccgtcgg cctggtgaag
     1561 ttcgagaatg gcgccgcgct ctgcctggag tcgagcttct gtgccaacat cgaaaaggac
     1621 gtgatggaca ccacgctcct cggcaccaat ggtggcgcct cggtcttccc gcccaccatc
     1681 atgcgcgagg aatccggcgc gctcaccgtg tcgcacatca cctcgttgcg cgatatccaa
     1741 tcgcatggcg agaacatccg ccgcttcatt caggcgattg tggatgatac cgagccgctg
     1801 gtcaccggcg agcaggccct gatggtggcg gagatcatcg acgccatcta tgaatcgcaa
     1861 gaaacgggca aggaggtacg cctctaacag acaggcgaat ggggcgcgcc ggagcggggt
     1921 ttcctttctc cggcgtgcct ccctccccgc atattccatc tcgcctatcc cgatggagag
     1981 aggtctggcc atgagatgct gcgcgttcct tctcgcgatg tgcgttgtgt tcgctccagc
     2041 aacccgtgcc gaatcggtct cccagacgga gatgctgctc accgagacgt tcgaaaccgt
     2101 cgcggacggc gcgttgccgg agggctggca gccggctggc gggaattggt cagtcaccca
     2161 ggggcgtctc cgcggtgagg cgaccgttta cctctcaaag atcttgctcc caacgccggc
     2221 gcagagcgac ctcgctttcg aggccgatgt cgcctttgag aaggccgccg atgccggcag
     2281 gtgggtcgcc ctcgccgtgc gcaatccgga aggcgcgggc gcgccccacc tccagttcac
     2341 ccatcgcttc gaccgccgtc aaccgaatgg cctcgagttc ggctggctgg cgagccacga
     2401 accggtcaaa tggtccgtct tgagcctggg atcggccgat atcgcccccg agctcggcaa
     2461 agcacaccgc ctccgctttg aagtgcgcgg ctggcaggcg cgcggtttca tcgatgacga
     2521 gctggtcctg gagcagccct tgcccctcag cacgctcgtc gcgggccgcc tggcgctgat
     2581 catcagcggg gccaccgcca ccttcgacaa catccgcgtc tggaagctgc ccgctctggg
     2641 cgcggaggag tttgaccggc tgttggagca gacgtgcacc actccgctcg tcatcgccca
     2701 ccggggcaac tcgctccatg cgcctgagaa caccctggag gcgatccgcc aggcaattga
     2761 agcaggtgcc gacctggtgg aggtcgatgt caacatgagc aaggatggcg tcgtggtgct
     2821 catgcacgac tataccgtcg accggaccac caacggcaag gggaaggtct cggatctgac
     2881 gctggccgag ctgaaggcgc tggatg
//
LOCUS       DAOSCV010000583         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22147_length_2903_cov_2.414677, whole genome shotgun sequence.
ACCESSION   DAOSCV010000583 DAOSCV010000000
VERSION     DAOSCV010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22147_length_2903_cov_2.414677"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1235
                     /locus_tag="PLY56_13540"
     CDS             354..1235
                     /locus_tag="PLY56_13540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOJ22550.1"
                     /translation="MRQRSRYAWVWVVVLSWAGMGTPGTPGPAVPRAGVPTRAAPGGG
                     ASEYEVAVGRSAARELEQRYGLCQDEKLNARVRRAAAPLLRVCGSRTPIVIRVLNSDE
                     PNGVALPGGFIFVTQGLLRILSEEDLLSAALGHEVAHIALGHMTEMLREYTNARMPRS
                     EGENSEYPRATGSGVPEPPNSLPPRLEAPKRASVNVVHEREKEADRAAARYLRAVGID
                     PRAVVRLLIRLSEQPELDASNSETHPTWPERIRSLEEYLNSPEFRNSGPRAPEALPPA
                     YPRGDLGVPWPLRAPAR"
     gene            complement(1243..2370)
                     /locus_tag="PLY56_13545"
     CDS             complement(1243..2370)
                     /locus_tag="PLY56_13545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ22551.1"
                     /translation="MKVLHVHPKIGIGGVETYLCRLIGEQVRLGHTVGLLTAGGVFEE
                     RVTAAGGRLFLIPPRKEHLESALEAIAPHHFDLIHAHNYRAARFARALAARAGARYLM
                     SVHGPRWFWHRLLFRGWSDSVIAMSEGDRDNITSLGGISRRRVHLSFYGIDTDRFRPG
                     IETHSLRAELGLPEDALPLVYISRFAHQKAGVALELLRAMPRICAAEPRAILLLVGEG
                     PMEDQLRAGVEQVNRQVGRRAAILVGPRRDIERVMNLGTLVIAAANTALEAIACGAPT
                     LAAGRTGYFGQVTVDNLEAARAVCFADHGRLAHRATEPHLARGILEILSNRIAAQAAA
                     AELSALVADRYNVARMAEDIEGVYQRLLNSRGTPSQAASIV"
     gene            complement(2501..>2903)
                     /locus_tag="PLY56_13550"
     CDS             complement(2501..>2903)
                     /locus_tag="PLY56_13550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOJ22552.1"
                     /translation="DEASGAQVIAFGGPGEEAATGDWLATQATNAVNLAGKLSLRDFA
                     AAVSRCTAFVGGDSGPTHIAAALGVPTVGLYGFTDPTRMGPLGERVEVLLGLERHWDP
                     NAQRLQESPYQWLDPISPEEVWKAIGRLTAR"
BASE COUNT          442 a          977 c          998 g          486 t
ORIGIN      
        1 gcgcgcttga tcgattgaat tggtacggtg gctgccatct ccctgcttcc agatagttga
       61 atgtcgttcc attatataga agcaccgggc gcctgtcaag gggtttttca tgggggaggc
      121 ggtgatcttt ccgatgcccg ggtcccagat ccggccacac gcgggtaagc accgggccgc
      181 tcgcatgtcg cgcggttctt tcgccccggg cgtgtggcgg atgccccgtc acccgtggta
      241 tcgatctccg ctccaggcgc cccccattct gacgccactg ggtggccgcc tgggccttta
      301 cattctggca cgttctctgc cccttccttg cctgcggcga gggaggagcg aggatgcgac
      361 agcgttcccg atatgcgtgg gtgtgggtgg tggttctgtc gtgggccggg atgggcacgc
      421 ctgggacgcc ggggccagca gtgccccggg ccggggtgcc aacacgggca gcgccgggcg
      481 gcggagcatc ggagtatgag gtggcggtcg gccgctccgc ggctcgggaa ctggagcagc
      541 gctacggact ctgccaggat gagaagctca acgcgcgtgt tcgacgcgct gccgcgcccc
      601 tgctccgggt atgtggcagt cggacgccca tcgtcatccg cgtcctcaac tcagacgagc
      661 cgaacggcgt cgccctcccc ggggggttca tcttcgtgac gcagggactg ttgcgcatcc
      721 tgagcgagga ggatctcctc tcggcggcgc tagggcacga ggtggcgcat atcgctttgg
      781 ggcacatgac cgagatgctg cgcgagtaca cgaacgcccg gatgccccga tccgagggag
      841 agaattcgga gtacccgcgg gccacaggct cgggagtgcc agagcccccg aattctctgc
      901 ccccgcggct tgaggctccg aagcgcgcct ccgtcaacgt ggttcacgag cgcgagaagg
      961 aggccgaccg cgccgcggcg cgttacctgc gcgccgttgg tatcgacccg cgcgccgtgg
     1021 ttcggctcct gatccggctt tcggagcagc cggagctcga tgcctccaac agcgagacgc
     1081 atcccacctg gccggagcgg atccgcagcc tggaggagta cctgaacagc ccggagtttc
     1141 ggaacagcgg tccgagggcg cccgaagcat tgcccccggc gtatcctcgg ggcgatctcg
     1201 gcgttccctg gccgcttcgg gcgccagctc gctagctcct gttcaaacga tgctcgctgc
     1261 ctgggaggga gtgccccggc tattcaggag acgctggtac acgccctcga tatcttccgc
     1321 catgcgcgcc acgttgtagc ggtctgccac cagggcggat agctcggccg cggcggcctg
     1381 ggcggcgatc cggttggata gaatctcgag gatgccgcgt gcgagatggg gttccgtcgc
     1441 gcgatgggcg agccggccgt ggtccgcgaa gcagacggcg cgcgccgcct cgaggttgtc
     1501 cacggtcacc tggccgaagt agccggtgcg cccggcagcc agggtaggcg caccgcacgc
     1561 gattgcctcc agcgcggtgt tggccgccgc gatcacgagg gtcccgaggt tcatgacgcg
     1621 ctcgatgtcg cgacgcgggc cgacaagtat agctgcccgc cgtcccacct ggcggttgac
     1681 ctgttctacc cccgcgcgga gttgatcctc catcggacct tctcccacga ggagtaggat
     1741 ggcccggggc tcggcggcgc agatccgtgg cattgcgcgc aggagctcga gcgccacgcc
     1801 tgccttctga tgcgcaaacc ggctgatata gacgagcgga agggcatcct cgggaagccc
     1861 cagctcggcg cggagcgagt gggtctcgat ccccggccga aaccggtcgg tatcaattcc
     1921 gtagaagctg aggtgaacgc ggcggcgcga aatcccgccg agcgacgtga tgttgtcgcg
     1981 gtcgccctcg ctcatcgcga tcaccgagtc actccagcct cggaagagaa ggcggtgcca
     2041 gaaccagcgc ggcccgtgaa cgctcatcag gtagcgggcg ccggcgcggg cggcgagcgc
     2101 cctggcaaaa cgcgccgcgc ggtagttgtg ggcgtgaatc agatcgaagt gatgcggggc
     2161 gatcgcttcc agagcggact ccaggtgctc cttgcggggg ggaatcagga agaggcggcc
     2221 cccggcggcg gtcacccgtt cctcaaaaac gcctcccgct gtcaacaggc ccaccgtatg
     2281 ccccaggcgg acctgctccc caatcagccg gcataggtaa gtctccacgc cgccaatgcc
     2341 gattttcggg tggacatgca gcaccttcat aggctctccg gcggtcaaaa ccgcctctcc
     2401 tttcgttctc cagttcgctc cgcgacagcc cgggctggca cccgagggcg acatggcgcc
     2461 tccccgcttc ctcccgcgct ggacgcgcga gtggcgttct tcagcgcgcg gtcaagcggc
     2521 cgattgcctt ccacacctcc tcgggcgaga tcggatccag ccattggtag ggcgactcct
     2581 gcaggcgctg ggcgttcggg tcccagtggc gctccagccc gaggagcacc tccacgcgct
     2641 ctccaagcgg gcccatgcgc gtcggatcgg tgaagccgta gaggcccacg gttggcacgc
     2701 ccagggcggc ggcgatgtgc gtggggccgc tgtcgcctcc aacgaacgcg gtacaccgcg
     2761 acaccgcggc ggcgaaatcg cgcagcgaga gcttcccggc caggttgacc gcgttggtcg
     2821 cttgtgtggc cagccaatcg ccggtggccg cttcctcgcc agggcccccg aaggcgatca
     2881 cctgggctcc actcgcctca tcc
//
LOCUS       DAOSCV010000584         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22192_length_2898_cov_3.896940, whole genome shotgun sequence.
ACCESSION   DAOSCV010000584 DAOSCV010000000
VERSION     DAOSCV010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22192_length_2898_cov_3.896940"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLY56_13555"
     CDS             complement(<1..194)
                     /locus_tag="PLY56_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HOJ22553.1"
                     /translation="MNVVVTGIGAVSAIGSGEAFFEALRQGASGITEVTILDPGESAS
                     TLAAEIAEFAVEEYLESAKT"
     gene            complement(191..1420)
                     /locus_tag="PLY56_13560"
     CDS             complement(191..1420)
                     /locus_tag="PLY56_13560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOJ22554.1"
                     /translation="MAPAQDGERRRVVITGLGAVSSLGPTVEALWEGLCAGRSGISPI
                     ERFDASGLRNATGGEVKELAALRLPPGVDPADLATRFALAAAREAVEDAGLTDRAGAE
                     RRGIAFATNFGGASLWERFSTSLMTAPEEADPADLAGYAFLSAAALAAECFGFAGPRA
                     TLSLSCSSGGAAIAWGLDTIRLGRADVVLAGGYDMLSLSALAGLSVLRTITAEKLRPF
                     DKKRSGTIFGEGAGMLVLEEREHALARGARIYGEVCGAATNNNAYHVTAPDKRGEGLT
                     QVVRAALADAAVAPEAIDYVNAHATGTKYHDVVETLAIKENLGERAYQVPVSSIKAAT
                     AHAMAAAGSLEAIACLLAIRDGVVPPTLNYEEPDPECDLDCVPNEAREARVTCALSIS
                     AGIGGNNAALILRRDEP"
     gene            complement(1452..1970)
                     /locus_tag="PLY56_13565"
     CDS             complement(1452..1970)
                     /locus_tag="PLY56_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOJ22555.1"
                     /translation="MVRITLEKLYPDMKEATVGRWLKSEGEPVAEGEPLVELITDKIT
                     FELESTGSGILRQIYFREKSVVPVGCTLAALGDSTEALPDIEAENRERIAAWEAEMAA
                     EREAAAPAGPRLVAGGPRLKRAEGVRATPSARRLAREHGIDLADVPPPAEGPITEKDV
                     QAYLEKSERGKR"
     gene            complement(2165..2800)
                     /locus_tag="PLY56_13570"
     CDS             complement(2165..2800)
                     /locus_tag="PLY56_13570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ22556.1"
                     /translation="MELNLALEKTTLAAGAAAKSAGQATPAIVDEDRGLSFGEITEKY
                     YRRIYNLICYQVGDLDEAADLTQETFVKAFRAYHAFKGESRVSTWLWRIALNMCKSSY
                     RKRARRERLIPLSIDADVVTDEGEMSREVPDWSVCPENVVETRELRAEVRRAIQSLAE
                     PYRVAIILCDIEGCSYQEIADITQISVQAVKSRIHRGRRMLRKKLERYVQG"
BASE COUNT          453 a         1063 c          887 g          495 t
ORIGIN      
        1 taggtcttgg cgctctccag gtactcctct accgcgaact ctgcgatctc cgccgcaagg
       61 gtggacgcac tctcgcccgg gtcgagaatc gtcacctcgg tgatgccgga cgcgccctgc
      121 cggagcgcct caaaaaacgc ctcgccgctc ccaatcgccg ataccgcgcc tatccccgtc
      181 acgaccacgt tcacggctca tccctccgca gtatcagggc cgcgttgttc ccgccaatcc
      241 ccgccgagat ggaaagcgcg cacgtcacgc gcgcctcgcg cgcctcgttg ggcacgcaat
      301 ccaggtcgca ctcggggtcc ggctcctcgt agttgagcgt tgggggcaca acgccatcgc
      361 ggattgccag cagacaggca atcgcttcca ggctgcccgc ggcggccatc gcgtgcgccg
      421 tggctgcctt gatcgagctg acgggcacct gataggcgcg ctcgcccaag ttctccttga
      481 tcgccagcgt ctctaccacg tcatgatact tcgtgcccgt ggcatgggcg ttgacgtaat
      541 cgatggcctc gggagcgacc gccgcgtccg cgagcgcggc acgcacgacc tgggtgaggc
      601 cctccccgcg cttgtcgggc gcggtgacgt ggtaggcgtt gttgttcgtg gcggcgccgc
      661 acacttcacc gtagatccgg gcgccccggg caagggcgtg ttcgcgctcc tccaggacga
      721 gcatcccggc gccctcgccg aagatcgtgc cgctccgctt cttgtcgaag gggcgcagct
      781 tctccgcggt gatcgtccgc aagacgctga gcccggcaag cgcggagagg ctgagcatat
      841 catacccgcc cgcgaggact acgtccgccc ggccgagccg gatcgtatcg agcccccacg
      901 cgatcgccgc gccgcccgag gagcaggaga gcgagagcgt ggcgcgcggg ccagcaaacc
      961 cgaaacactc tgcggcgagc gcagccgcgc tcaggaaggc gtagcccgcc agatcggcgg
     1021 ggtctgcctc ttcgggcgcc gtcatgaggg aagtggagaa gcgttcccag agcgacgcgc
     1081 cgccgaagtt ggtcgcgaac gcgatccccc ggcgctctgc tccggcacga tcggtaagcc
     1141 cagcgtcttc cacagcctcg cgcgccgcgg cgagcgcgaa gcgcgtggct aggtcggctg
     1201 ggtccactcc cggcggcagc cgcagcgccg ccaactcctt cacctcgccg cccgtggcgt
     1261 tgcgcaggcc ggacgcatcg aaacgctcga tcggcgagat cccggagcgc ccggcgcaaa
     1321 ggccctccca cagcgcctcg accgtcggcc ccagggagct tactgcgccc aacccggtga
     1381 tgacgacccg ccggcgctcg ccatcctggg ctggagccac cctctctgcc gcgcttcttg
     1441 tttccgaagt gctaccgctt tccacgctca ctcttctcca ggtaggcctg aacgtctttc
     1501 tcggtgatcg gaccttccgc ggggggagga acgtccgcca ggtcgatgcc gtgctcgcgc
     1561 gccagccgcc gggccgaggg ggtggcgcgc accccttccg cgcgcttcag gcgcgggccg
     1621 ccagccacca ggcgcgggcc cgcgggcgcg gcagcctcgc gctccgcggc catctctgcc
     1681 tcccaggcgg caatccgctc gcggttctcc gcctcgatat ccggaagcgc ctccgtcgaa
     1741 tcgcccagcg cggcgagcgt gcatcccaca gggaccacgc tcttctcgcg gaagtagatc
     1801 tgccggagga tcccggagcc ggtcgactcc agttcgaagg taatcttatc ggtgatcagc
     1861 tcaacaagcg gctcgccctc ggccaccggc tcgccctcgc tcttcagcca gcggccgacg
     1921 gtcgcctcct tcatatcagg gtagagtttc tccaaggtga tccggaccac tctccggcat
     1981 cctttccagc gtctcccgaa agctccaccg ccccacccag cagtggatgg aaccggaacc
     2041 tacagacgca tacagccggc aggagccggc tgtttcctct tctcccaaca gggcgcgctc
     2101 catcagggaa cccgcgcggg tgaagccacc cgtctccatc gcgccgctca ggccgcccgg
     2161 ccgcctatcc ctgcacgtag cgttcgagct tcttgcggag catgcgccgc ccgcgatgaa
     2221 ttcgtgactt cactgcctgg acactgatct gggtgatgtc ggcaatctcc tgatacgagc
     2281 acccctcaat atcgcacagg atgatcgcca cgcgatacgg ctcggccagc gattggatcg
     2341 cccggcgcac ctccgcgcgc agctcgcgcg tctctaccac gttctctgga caaacggacc
     2401 aatcgggcac ctcacggctc atctcgcctt cgtccgtgac cacgtcggca tcgatggaga
     2461 gggggatgag ccgctcacgg cgggcacgct ttcggtagct gctcttgcac atgttgagcg
     2521 cgatgcgcca gagccaggtc gagacccgcg actcgccctt gaaagcgtgg taggcacgaa
     2581 acgccttgac gaacgtctcc tgggtcagat cggccgcttc gtccagatca cccacctggt
     2641 agcaaatcaa gttataaatg cggcggtagt atttctcggt gatctccccg aagctgagcc
     2701 cgcgatcctc atcaacaatc gcgggcgtcg cttgcccggc cgacttcgcc gcggcccctg
     2761 ccgcgagcgt tgttttttcc aaggccaggt tcaactccat gaggcgcctc cattagagcc
     2821 tgtccgcgcc gttcatcggt ccgatcagga cccgcctgtg ggatccttcg ccggtgggag
     2881 ccctatttcc tttgcctg
//
LOCUS       DAOSCV010000585         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22205_length_2897_cov_3.332160, whole genome shotgun sequence.
ACCESSION   DAOSCV010000585 DAOSCV010000000
VERSION     DAOSCV010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22205_length_2897_cov_3.332160"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1762)
                     /gene="dnaA"
                     /locus_tag="PLY56_13575"
     CDS             complement(389..1762)
                     /gene="dnaA"
                     /locus_tag="PLY56_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ22557.1"
                     /translation="MTKQLHIDGLFASAEELWESVLAALRRLPDKRLQDECLQGSRAV
                     RLEDGTLAVEVTNRYSPDFVEKRCRRAVEEILERLVGSTLPLCFRAGAEAPEEADTPP
                     ETRRPAPAPKPASSAGQRLNIKYRFDTFAVGQSNTLAWRAAMAVANAPASTYNPLLLC
                     GGVGVGKTHLLQAIGHEIARLFPSQHVVYVTADAFTYEFVTALRERRTDAFRKRYRGA
                     DVLLVDDLQFLAGKESTEEEFFLTFTAVQEAGKQIVCCSDRCPREMPLLPDRLRSRLE
                     SGLIVEIGAPDFETRVQILRRKAEAEGIEIDEEVLLFVANLIESDVRALEGALIKLIA
                     CSSLTRRPLTCALAEETLVSYVGRKSSSSVTVETVLRATCDFYRVDAKAVKSKRRDKS
                     VVLPRQVAMYLSRQITNAPLAEIGERFGGRDHSTVIAACNKIKGSLESDTELRAAIEE
                     ISRRLAG"
     gene            complement(1978..2250)
                     /locus_tag="PLY56_13580"
     CDS             complement(1978..2250)
                     /locus_tag="PLY56_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22558.1"
                     /translation="MKKVHRSVPDVPQAVADQGADILERYFNRYGVTRATHLPEPAKI
                     EMMRELRAFYSVELPQGWLEKARQRAQQRAWWQRMAIWFREALGLE"
     gene            complement(2448..2699)
                     /locus_tag="PLY56_13585"
     CDS             complement(2448..2699)
                     /locus_tag="PLY56_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22559.1"
                     /translation="MQLDAPILDTTVEDAVEAPETTIPEVDPLVETKKAAEMVKLIDL
                     HLDYERQSRIPLSMDELTPRRLCPEQRVDLVTDLYAVTH"
BASE COUNT          496 a          976 c          946 g          479 t
ORIGIN      
        1 gggctgggag gggaagcccc gacaggggct ttgctcagaa gcagcgcgga gcttcgcgcc
       61 cgcgtgccgg gctgctttag tatccgccct gccacgggca cagggcgaac agtgaacgtt
      121 ctcccctacc tgtcgccggg gcttcggtag cgggggcctg ggaggggaag cccgacaggg
      181 gctttgctca gaagcagcgc ggagcttcga gctccgcgcc cgaggtctgg ttactatccc
      241 gcgtgcctca caacggcaag ggctgggagg ggaagccccg acaggggctt tgctcagaag
      301 cagcgcggag cttccagctc cgcgcgtgcg ggctggccgc actcccgcgt gcctgagaac
      361 ggcaagggct gggaggggga gcccccgctc agcccgcgag ccggcgcgag atctcctcga
      421 tcgcggcgcg gagctccgtg tcgctctcca ggctgccctt gatcttgttg cacgccgcga
      481 tcacggtgga gtggtcgcgc ccgccgaagc gctcgccaat ctccgcgagt ggcgcgttcg
      541 tgatctgccg cgagaggtac atcgccacct ggcgcggcag caccacgctc ttatcgcggc
      601 gcttgctctt gacggctttg gcatccaccc ggtaaaaatc gcacgtggca cgcaggaccg
      661 tctccaccgt gacgctcgag ctgctcttgc ggcccacgta gctcacgagc gtctcctcgg
      721 caagcgcgca ggtgagcggc cggcgcgtga gagacgagca ggcgatcagc ttgatcagcg
      781 ccccctccag cgcgcgcacg tcggattcga tcaggttggc cacgaaaagg agcacctctt
      841 cgtcgatctc tatcccctcc gcctcggcct tccgacggag gatctgcacc cgggtctcaa
      901 aatcgggcgc gccgatctcg acgatcagcc cgctttccag gcgcgagcgg agacggtctg
      961 gcaagagcgg catctcgcgc ggacagcggt cgctgcaaca gacgatctgc ttccccgcct
     1021 cctgcacagc ggtgaaggtg aggaagaact cctcctccgt gctctccttg ccggccagga
     1081 actgcagatc atctacgagg agcacgtcgg cgccgcggta gcgtttccgg aaggcatcgg
     1141 tgcgccgctc gcgcaacgcc gttacaaact cataggtaaa cgcatcggcg gtgacgtaga
     1201 ccacgtgctg gctggggaag agccgggcga tctcgtgacc aatcgcctgg agcaggtggg
     1261 tcttgcccac gcccacgcct ccgcacagga gcaacgggtt gtaagtagat gcgggggcgt
     1321 tggcaacagc catcgccgcg cgccaggcca gcgtgttgct ctggcccacg gcgaaggtat
     1381 caaagcggta ctttatgttc aggcgctgac cggcggaaga ggccggcttg ggcgccggcg
     1441 cggggcgccg cgtctcgggc ggggtgtccg cctcctcggg agcttccgcg cctgcccgga
     1501 agcacaaagg caacgtgctc ccgaccaggc gttccaggat ctcctccacc gcgcgccggc
     1561 accgcttctc cacgaaatcc ggcgaatagc ggttcgtcac ctccaccgcc agcgttccat
     1621 cttcgaggcg cacggcgcgc gagccctgaa ggcactcgtc ctggagccgt ttgtccggca
     1681 ggcggcgaag agccgcgagt acactctccc acagctcttc ggcgctggcg aacaacccat
     1741 caatatgcaa ctgcttcgtc atagaacgtc cggatcagag acggaagaga cccaggcgca
     1801 cagccagcgt gagggtagga agtccagcgc aactgggcga tgtgcgtcca ttatagcaca
     1861 tccagcgaat cggtgggagg ggcgggcctg gagggagatc ctaagcggga cgcgccctcg
     1921 cgcgattctg gggggaattg ggggagtggc aaaaagggaa ccggaggccc tggggcccta
     1981 ttcgagaccc agggcttcac ggaaccagat cgccatgcgc tgccaccagg cacgctgctg
     2041 ggcgcgttgc ctggccttct ccagccagcc ttggggcagc tccaccgagt agaaggcgcg
     2101 cagctcgcgc atcatctcga ttttggcggg ctcgggcagg tgggtcgctc gcgtcacccc
     2161 gtagcggttg aagtaacgct ccagaatatc cgccccctga tcggcgacag cctggggcac
     2221 atccggtacc gagcgatgaa ctttcttcat tgtctgcctg cgcctctcaa gaagaagcag
     2281 gctgccttgc tggccgcgcg ggcgtgacac ccgcatccgc ctagcctagg gaaagcgttc
     2341 gggccaggcg ggcgggcccg ctcccaggcc ggcgccgccc gccctcccac gctgccggtc
     2401 ggggcagcct gcaacaggct cctttgtcgg agcacctttt cccccattca gtgggtgaca
     2461 gcgtacagat ccgtcaccag gtccacgcgt tgttccgggc aaagccgcct cggtgtcagc
     2521 tcatccatag agagaggaat gcggctctgg cgctcgtagt ccagatgcag gtcgatcagc
     2581 ttgaccattt cggccgcctt cttcgtctcg accagcggat ccacctccgg gatcgtcgtc
     2641 tcgggtgctt ctaccgcatc ctcgactgtc gtatccagaa tcggcgcatc caattgcatg
     2701 acagctctcc ctccttgttg cttggtccgg agttaccccg gtgtcgaatc cactaaacgc
     2761 acgggcggcg cgccgcgcgc cgcgcgccac gctcggtggc acacctcggc tccacgcgaa
     2821 cagagtcggc tgcacaaaag aagccaacag gagtcggccc aggcgcgagc ctaagcccca
     2881 cccggacggc ggtccac
//
LOCUS       DAOSCV010000586         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22262_length_2892_cov_3.291858, whole genome shotgun sequence.
ACCESSION   DAOSCV010000586 DAOSCV010000000
VERSION     DAOSCV010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22262_length_2892_cov_3.291858"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLY56_13590"
     CDS             <1..243
                     /locus_tag="PLY56_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22560.1"
                     /translation="YTNELKRVRHHAPFSDTVKGGQGLGHFGGDTALMENFLDVIRGR
                     AHSRTPIQTGIQSVYACLAAKESAETGRFVDVRQLG"
     gene            703..1155
                     /gene="mraZ"
                     /locus_tag="PLY56_13595"
     CDS             703..1155
                     /gene="mraZ"
                     /locus_tag="PLY56_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ22561.1"
                     /translation="MFIGEYEHSLDDKGRIVLPIKFRYSLGEKFFITKGLHGCLFIFP
                     EPEWTKFNQSLSEQPVVNLDATRLQRFFSASAFEVTTDSQNRLAIPAVLRKYAQIEDQ
                     AVVVGAANRVEIWNPERWEAVNSDITPEEIAASAQALGLSGSNGAPVA"
     gene            1148..2119
                     /gene="rsmH"
                     /locus_tag="PLY56_13600"
     CDS             1148..2119
                     /gene="rsmH"
                     /locus_tag="PLY56_13600"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041245588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ22562.1"
                     /translation="MPDRLPSGPSSSPYHVPVLLAQVLTFLRPRPGGIYVDGTLGDGG
                     YAEAILRESSPDGIVIGIDRDPEALATARSRLSEYGDRLRTVHATYDRVAEVVHAQGL
                     AAVQGVVFDLGVSSRQLDQAVRGFSFRQDAPLDMRMDTTAGVTAAELVNTLPERELER
                     VLREYGEERLAGRIARAIAHERALAPIETTGRLAQIIWDAVPASYRHGRIHPATRSFQ
                     AIRILVNREIDLLEGGVRGGVEILEPGAPIVVVAYHSLEDRVIKRLFRELAGKGFPAG
                     EAPTPTVRLLTPKPLTPEPAEVAANPRARSAKLRAAEKLPPPDVGGP"
     gene            2167..2592
                     /locus_tag="PLY56_13605"
     CDS             2167..2592
                     /locus_tag="PLY56_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22563.1"
                     /translation="MEVLVVNAVPENVTRMISTGSQTSAATPAPRPRRRRRANSRRAA
                     LLLGSCTLLAALPVVYVASYASATRHSYQHSRLQARLHQLQEDRKLLEAQVADLQRTD
                     RVLYEAKRIGMQPREALQYVAIVPSKAPAREIRQASARP"
     gene            2611..>2892
                     /locus_tag="PLY56_13610"
     CDS             2611..>2892
                     /locus_tag="PLY56_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22564.1"
                     /translation="MRARRQPYIDTIKRRTEWAFCFIFVWFLFLSYQLFNIQVRQHAH
                     FKKQAARYHNRRIQVDAVRGILYDRNGTALTQNLPLKSVYADPSLIDDPA"
BASE COUNT          479 a          972 c          942 g          499 t
ORIGIN      
        1 tacaccaacg agctgaagcg ggtgcgccat cacgctccgt tcagcgatac ggtgaagggc
       61 gggcaagggc tcggtcactt cggcggcgat acggcgctga tggagaactt cctggacgtg
      121 atccgtggcc gcgcgcattc gcgcacgccg atccagaccg gcatccagag cgtctacgcg
      181 tgcctggcgg ccaaggaatc ggccgagacc gggcgcttcg tggacgtgcg ccagctcgga
      241 taaccagagg cacagaggtg aaggggcgtt gccggacgcg gtgacgcccc tctcccctcg
      301 ccgccgggtg gcgcagcgag gcttttcgcg cgtccgtctc cgacgctcgc atccccctcc
      361 atccagaggc cttctgcttg ccgagcgtat tcacggtccc ggcgccagtc ccactcgtcc
      421 ttggtgcccc attcccccgg ataggaccgt ccccaggctc gccgcctctt ctgcgcgctc
      481 cctcgccctc cgaaaggcac gcgcgctctc tacagaacag ccgccggcgg gatcgcgctt
      541 ccttctcacc cgcctgtacc ccacgcgctc gcgtggcaca ccgccgtcct cccatcgtgt
      601 gaagattgcc cccacgtacc tattgacagg catgtggtga ctaaggtata ctgcggtgga
      661 gagccagggg gaacctggtg gagacccggt ggagaccccg cggtgtttat cggcgaatac
      721 gagcatagcc tcgacgacaa ggggagaatc gtcctcccaa taaagttccg ctattcgctc
      781 ggtgagaagt tcttcatcac caagggcctg cacgggtgtc ttttcatctt ccctgagcca
      841 gagtggacca agttcaatca gagcttgagc gagcagcctg tcgtcaacct ggatgcgacg
      901 cggttgcagc ggttcttctc ggcgagcgcg tttgaggtga cgacggatag ccagaaccgt
      961 ctggcgattc cggcggtact tcggaagtac gcccagatcg aggatcaggc cgttgtcgtg
     1021 ggcgccgcca accgcgtgga gatctggaat ccggagcgat gggaagcggt gaatagcgat
     1081 atcactccgg aagagatcgc ggcctcggca caggctctcg gcttgagtgg gagcaacggt
     1141 gcgccggttg cctgataggc tgccatccgg gccctcgtcc agcccctatc atgtgcccgt
     1201 tctcctcgcg caggtcctca cctttctgcg gccgcgcccg gggggcatct acgtcgatgg
     1261 cacgttgggc gatggcggct acgccgaggc gatccttcgc gagagcagcc cggacggcat
     1321 cgtcattggc attgaccggg atcccgaggc actagctacc gcgcgatcac ggctctcgga
     1381 gtatggagac cggctgcgca cggtccacgc gacctatgac cgggtggccg aggtggtcca
     1441 tgctcagggc ctggccgcgg tgcagggagt ggttttcgac ctgggggttt cctcccgcca
     1501 gctcgaccag gcggtgcggg gcttctcctt ccggcaggat gcgccgctcg acatgcggat
     1561 ggatacgacg gcgggcgtca cggccgcgga gctcgtgaac acgctcccgg agcgggagct
     1621 ggagcgcgtc ctccgcgagt atggcgagga gcggctggcc ggccggatcg cccgggcaat
     1681 cgcgcacgag cgcgccctgg ctccgattga gacgacaggt cggctcgccc agatcatctg
     1741 ggacgcggtg cccgcgagct accggcatgg gcgcatccac ccggcgacac ggagctttca
     1801 ggcgatccgg atcctggtga accgtgagat cgatttactg gagggcggcg tgcggggcgg
     1861 cgtcgagatc ctggagccgg gcgccccgat agtcgtcgtg gcttatcact cgctggaaga
     1921 ccgcgtgatc aagcgtcttt tccgtgagtt ggccgggaag ggcttccccg ccggggaagc
     1981 gcccactccc acggtgaggc tactcacccc caaaccgctc acgcctgagc cggccgaggt
     2041 ggccgccaac ccgcgggcgc gcagcgcgaa actgcgcgcc gccgagaagc tgcctccccc
     2101 ggatgttggc gggccgtgac ccggcgcgcg ggtgtctggt agcggcaggg gtaagaaata
     2161 actgcgatgg aggtgctggt cgtgaacgct gtgcccgaga acgtaacccg gatgatttcg
     2221 acggggtcac aaacctccgc cgcgaccccg gcgccccgcc ccagacggag gcggcgcgct
     2281 aactcgcgcc gtgccgccct gctgctcggc tcgtgtaccc tgctggctgc ccttccagtg
     2341 gtctacgtcg cttcgtacgc cagtgccacc cgccactcct atcagcatag ccggctgcag
     2401 gcccgcctgc atcagctcca ggaggaccgc aagctgctgg aggcgcaggt ggcggatctc
     2461 cagcgcaccg accgcgttct ctacgaggcc aaacgaatcg ggatgcagcc gcgcgaagcc
     2521 ctgcagtacg tcgccatcgt cccgtcaaag gcgcccgcgc gcgagattcg ccaggcctcc
     2581 gcccgaccgt aacacgggag ggtggtttca atgagggcgc gtcgccagcc atacatcgat
     2641 acgatcaagc gccgcacgga gtgggctttc tgcttcatct tcgtctggtt ccttttcctg
     2701 tcgtaccagt tgttcaacat ccaggtgcgg cagcatgccc acttcaagaa gcaggcagcg
     2761 cgctaccaca accggcgcat tcaggtggat gccgtccgcg gcatcctcta cgaccgcaac
     2821 ggcaccgccc tgactcagaa cctcccactg aagtcggtct atgccgatcc ctcgctgatc
     2881 gatgacccgg cg
//
LOCUS       DAOSCV010000587         2891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22275_length_2891_cov_2.820169, whole genome shotgun sequence.
ACCESSION   DAOSCV010000587 DAOSCV010000000
VERSION     DAOSCV010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22275_length_2891_cov_2.820169"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PLY56_13615"
     CDS             <1..1126
                     /locus_tag="PLY56_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ22565.1"
                     /translation="AALYALAPVAGGVGALVGALTALFAATIALTQFDLKRVLAYSTI
                     SQLGLMFLAVGVGAYTAAIFHLTTHAFFKGLLFLGAGSVMHAMGNRIDLREMGGLRRY
                     LPATHWTFLAGTLAISGFPLLAGWWSKDEILAEAFHLGTRVWWAPGWLLWALGSLASL
                     LTAFYMVRAYTLAFHSDPRIDPEVEAHLHESPRSMTGPLWVLAGLSIVGGAALALPEL
                     WGHAGPVEAFLAPVWAHASGVHAAEAGHATGPAWGLMGVATLLAFAGMGAAWSAYRQR
                     LPSPEPLERARPMWNLFDQKWYLERGYHALFVRGGTWLAGVLAGAVDQKGVDRAVVGI
                     GSLVALTGEGLRRLQTGYVRSYALSIVVGAVLLAAYFMGR"
     gene            1237..2772
                     /locus_tag="PLY56_13620"
     CDS             1237..2772
                     /locus_tag="PLY56_13620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOJ22566.1"
                     /translation="MLLVVPVLGAVLLGVLVPRQNGLALKAGGAGVALVNLILAVVLW
                     ARFDPAQPGLQFEENVPWIRALSFQVNYHLGVDGISLLLVALTAFLMVLAFLGSWNVT
                     ERVKEYVITLLVLESAMLGVFLAADLLLFYIFWEVTMIPMFLLIGIWGGARRIYASIK
                     FVLFTAAGSLVMLVAMVSLAWTYSATTGQTTFDLEPVLRMRLPVATQVWLFAAFALAF
                     AIKVPLFPLHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFCLTLFPEASYRFAPI
                     LVALALVGIVYGALVALAQQDIKKLVAYSSISHLGFVMLGLFALNAPGLQGSLLQMIN
                     HGISTGALFLLVGMIYERRHTRMISDYGGLWKQMPLFSALFLVVALSSIALPFTNGFV
                     GEFLILLGAFNARVVWAAIAVSGMILSAAYMLWMYQRVFFGPLENPENRNLADLNRRE
                     LAILVPIVVLIFSIGVAPRFFLRPAEPALQMVLMRLPQPGAPMLSDARVAPALEDGRE
                     ALR"
BASE COUNT          368 a          908 c         1030 g          585 t
ORIGIN      
        1 cgcggcgctc tatgccctgg cgccagtggc gggcggcgtg ggcgccctgg tgggcgcgct
       61 cacggcgctc ttcgcggcca ccatcgcgct gacgcagttc gatctcaagc gcgtgctcgc
      121 ctattcgact atcagccagc ttggcctgat gttcctcgcc gtcggcgtgg gcgcctacac
      181 ggcggcgatc ttccacctga cgacgcacgc cttcttcaag ggtctcctct tcctcggcgc
      241 cggaagcgtg atgcacgcga tgggtaaccg gatcgacctc cgggagatgg gcgggctgcg
      301 ccgctatctg cccgcgacgc attggacctt cctcgccggc acgctggcaa tcagcggttt
      361 tcctctcctg gccggctggt ggagcaagga tgagatcctg gcggaggcgt tccatctggg
      421 cacacgcgta tggtgggcgc ccggatggct tctgtgggcg ctcggcagcc tagcgtccct
      481 gctcaccgcg ttctacatgg ttcgcgccta cacgctggcg ttccacagcg atccccggat
      541 cgacccggag gtggaagcgc acctccacga gtcgccacgg agcatgaccg gcccgctctg
      601 ggtgctggcg gggctttcca tcgtcggcgg cgccgccctg gccctaccgg agctgtgggg
      661 ccatgcgggg ccggtcgagg cgtttcttgc cccggtctgg gcgcacgcgt ccggcgttca
      721 cgcggcggag gccggccacg ctaccggccc ggcgtggggg ctgatggggg tcgccacact
      781 gctggcgttc gccggcatgg gcgccgcctg gtcggcctac cggcagcggc tgccatcgcc
      841 cgagccgctg gagcgtgccc ggccgatgtg gaacctcttc gaccagaagt ggtacctgga
      901 gcggggctat cacgcgctct ttgtgcgtgg cggaacttgg ctggctggtg tgctcgccgg
      961 agccgtggac cagaagggcg tggatcgagc ggtcgttggt attggcagcc tcgtggcgct
     1021 gacgggagag ggattgcgcc ggcttcagac gggctacgtg cgctcgtacg cactctcgat
     1081 tgtcgtgggc gcggtgttgc tggcggccta cttcatgggc cggtgagagg ggttgggaac
     1141 cgatggtgaa cagggggaga gactggcgag gagggcgagt tcgccggcgg ttcgcgagac
     1201 ggggcgctcg ggggtgaggc gatgcctttg ttgacgatgc tactggtggt gccggtgctg
     1261 ggagcggtgc tcctgggggt gttggtaccg cggcagaatg gcctggcgct gaaggccggc
     1321 ggcgcgggcg tggcgctggt caacctgatc ctggccgtcg tgctgtgggc gcgcttcgat
     1381 ccggcgcagc cggggctgca attcgaggag aacgtgccct ggatccgagc cctgagcttc
     1441 caggtgaact accatctggg cgtagatggg atcagcctgc tcctggtcgc gctcaccgcg
     1501 ttcctgatgg tgcttgcctt cctcgggagc tggaacgtca ctgagcgcgt gaaggagtac
     1561 gtgatcactc tcctggtcct ggaatcggcg atgctcggcg tcttcctcgc cgccgacctc
     1621 ctccttttct acatcttctg ggaagtaacg atgattccga tgttcctcct catcggaatc
     1681 tggggtggcg cccggcggat ctacgcctcg atcaagttcg tcctcttcac cgctgccggc
     1741 agcctggtga tgctggtggc gatggtctcg ctggcgtgga cctacagcgc gacgaccggg
     1801 cagaccacgt tcgacctgga gccggtgctc cggatgcggc ttccggtggc gacgcaggtg
     1861 tggctcttcg cggcgttcgc gctggcgttt gcgatcaagg tgccgctctt cccgctccac
     1921 acctggttgc cggacgcaca cgtcgaggcg ccgacggcgg gcagcgtgat cctggcgggc
     1981 gttctcctga agatgggcac ctacggcttc ttgcgcttct gcctcacgct cttcccggag
     2041 gcgtcgtatc ggttcgcgcc aatcctcgtg gcgctcgcgc ttgtcgggat cgtctatggg
     2101 gcgctggtgg cgctggcgca gcaggatatc aagaagctgg tcgcgtactc gagcatcagc
     2161 cacctcggct tcgtgatgct cggcctcttc gcgctgaacg ccccggggtt gcaggggagc
     2221 ctgctgcaga tgatcaacca cgggatcagc accggcgcgc tctttcttct cgtcgggatg
     2281 atctacgagc gccggcacac gcgcatgatc agcgactatg gcggcctgtg gaagcagatg
     2341 cctctcttta gcgcgctctt cctggtcgtc gcgctttcgt cgatcgcgct cccgttcacg
     2401 aacgggtttg tcggcgagtt cctgatcctg ctaggtgcgt tcaacgcgcg ggtggtctgg
     2461 gccgcgattg ccgtctccgg gatgatcctc tcggcggcct acatgctctg gatgtaccag
     2521 cgcgtcttct ttggcccgct agagaacccg gaaaaccgga acctggcgga tctcaatcgg
     2581 agggagctgg cgattctggt gccgattgtc gtgttgatct tctccatcgg cgtggcgccg
     2641 cgcttcttct tgcggccggc cgagcccgcg ctgcagatgg tgctgatgcg cctgccacag
     2701 cccggcgcgc caatgctgag tgatgcccgg gtggcacccg ccctcgagga tggaagggaa
     2761 gcgctgagat gagcacggcc tggaaggatc tcgcctcgct ggctcccgag gggatcgtgg
     2821 cgctgggcgc cctcgtcgtg ttgttcctcg acttggggga acgcaaggag cggcggcgcc
     2881 tggtcgccgg c
//
LOCUS       DAOSCV010000588         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22335_length_2885_cov_3.508481, whole genome shotgun sequence.
ACCESSION   DAOSCV010000588 DAOSCV010000000
VERSION     DAOSCV010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22335_length_2885_cov_3.508481"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLY56_13625"
     CDS             <1..915
                     /locus_tag="PLY56_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22567.1"
                     /translation="YSLFFTFLGCAGVAQGALLGKIIPAGRRGSALSAAHTISGPVNL
                     LAILGVYSLLRSGAFPTPRNYAFSFTVTTFLFVLSGLAIVRAHEKPSDCRDGSAGLRA
                     RLRYARKMVDTNRDYRILLLIQILLGFGGGALSFYTAYGKQVGSIHDSNVLLATFLQV
                     LFQSSAAAFLGRVADRRGNRAVIRSLLWVEAMTPVSTVLTATLFPGTPAYLLVYCMVG
                     LRFPVFDVITNYLLEIVPQEDHALALGTANTILLVTVGSPLILGMIAGYAGYPVIMLT
                     AAAILCAAAIAAFYLGEPRARASATTRL"
     gene            complement(970..>2885)
                     /locus_tag="PLY56_13630"
     CDS             complement(970..>2885)
                     /locus_tag="PLY56_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOJ22568.1"
                     /translation="FQERGETRPEELLCIVTAGLGAGASNCSGSDKQTYYKLGIQGKT
                     PDTPHDFALTLTQGGCTHGDLALIEGENTLREFYHLVEKGVPFPHNERGGFVGYKTDH
                     DPRQRATSAGPWTSRFMVQKSLAQVRRFGIPILAPYEAIALLTVGEGKARRCIGVLAV
                     NHKDAEEEGFGLTLFNAENVVMATGGPGEMYAVSVYPRYQMGAHGICFEAGAVAHNLT
                     ESQFGLASLTPRWNLSGTYQQVIPRYYSTDPDGSNPREFLNDYFPSMRALATAIFLKG
                     YQWPFDPDKVAGFGSSLIDILVEHERRDLGRRVWMDFTRNPMATGRLEPFSLSDLEPE
                     ARLYLERSGALQETPIQRLERMNPPSIELYRNRGVDLTREPLEIAVCAQHNNGGFAVD
                     RWWESNVSHLFVIGEQAGTHGVKRPGGSALNAGQVGGLRAAQRIAHAYGPRVPSVSEF
                     LSAVGDTVAAKLEAIRAGIRRAESAPRRAEEVQREIQQRMTDFGGVSRSLEGAARAVD
                     AASRLLVEIRRDGLRIERRRHFLRQLRVEAMCVAHLGYLTAIRDLIARGSGSRGSHLV
                     ADPKGILPHPALGSEWRFGPENPALREEILEVWLGEDGEFHTRAVPVRPIPESEFWFE
                     NTWEAYRSGRVFE"
BASE COUNT          446 a          969 c          959 g          511 t
ORIGIN      
        1 tactccctct ttttcacctt cctcggatgc gcgggcgtgg cgcagggggc gctcctgggc
       61 aagatcatcc ccgcgggtcg gcgcggctcc gcgcttagcg cggcacacac gatctcaggc
      121 cccgtgaatc tcctggcgat cctcggcgtc tattcacttc tgcgctcggg ggcgtttccc
      181 acgccgcgga actacgcgtt ttccttcacc gtgaccacgt tcctcttcgt gctctccgga
      241 ctggcgatcg tgcgggcgca tgagaagccc tcggattgcc gggatgggag cgccgggcta
      301 cgcgcgcgcc tcaggtacgc gcggaagatg gtggacacca accgcgacta ccgcatcctg
      361 ctgctcatcc agatcctgct ggggttcggc gggggagcgc tctccttcta taccgcctac
      421 ggcaagcagg tgggcagcat tcacgactcc aacgtcctcc tggccacctt cctgcaggtc
      481 ctcttccaat ccagcgccgc cgccttcctc gggcgagttg ccgaccgccg gggcaaccgc
      541 gcggtgatcc gcagcctcct gtgggtggag gccatgacac cggtgagcac cgtgctgaca
      601 gcgacgctct ttccaggtac ccccgcttac ctgctggtgt actgcatggt gggcctgcgc
      661 ttccccgtct tcgacgtgat caccaactac ctgctcgaga tcgttcctca ggaagaccat
      721 gcgctcgcgc tcggcacggc aaacacaatt ctcctggtga cggtgggctc gccgctcatc
      781 ctcggcatga tcgccggata cgcgggctat cccgtcatca tgctcaccgc ggccgcgatc
      841 ttgtgcgccg ccgccatcgc tgccttctac ttgggcgagc cgcgggcgcg cgcctccgcg
      901 acaacgcggc tgtaatcgcg gcttcgcgcg ccggcagggg cgttgttcga caggcgttcg
      961 gggagagggc tactcgaaaa cgcgcccgga gcgatacgcc tcccaggtgt tctcgaacca
     1021 gaactccgac tcgggaatgg ggcgcacggg tacggcgcgg gtgtggaact cgccgtcctc
     1081 accgagccag acctctagaa tctcctcgcg cagggcgggg ttttccggac caaagcgcca
     1141 ctcgctgccc aacgcggggt gggggaggat ccctttcgga tcggcgacga ggtgcgagcc
     1201 gcggctgccg ctcccgcgcg caatcagatc gcgaatggcg gtcaggtagc ccagatgcgc
     1261 cacgcacatc gcctcgacgc ggagctggcg caggaaatgc cggcgccgtt cgatccggag
     1321 gccatcgcgg cgaatctcga ccaggaggcg gctggccgca tccaccgcgc gcgctgcccc
     1381 ctccaggctg cgcgagacgc cgccgaagtc ggtcatgcgc tgctgaatct ctcgctgcac
     1441 ttcttcagcg cggcgcgggg cgctttcggc gcggcggatc ccggcgcgga tcgcctccag
     1501 cttggcggct acggtatctc caacggcgct caggaactcg gaaacggagg gcacgcgggg
     1561 gccataggcg tgcgcgatcc gctgggcggc gcgcagtccg cccacctgcc cggcgttgag
     1621 cgccgagccg ccggggcgct tcacgccgtg cgtgcctgcc tgctcaccga tgacgaagag
     1681 gtgagatacg ttgctctccc accagcggtc cacggcaaac ccgccattgt tgtgctgggc
     1741 gcagacggca atctccagcg gctctcgcgt caggtccacg ccgcggttgc gatacagctc
     1801 gatgctcggc gggttcatcc gctccaggcg ctggatcggt gtctcttgca gggcaccaga
     1861 gcgctccagg tagaggcggg cctcgggctc caggtccgag agggagaacg gttccagccg
     1921 gcccgttgcc atcgggttgc gcgtgaaatc catccagacg cgccggccga ggtcgcggcg
     1981 ctcgtgctcc accagaatat cgatgagcga ggagccaaac ccggccactt tgtccggatc
     2041 gaacggccac tggtagccct tgaggaagat agcggtcgcc agggcgcgca tgctagggaa
     2101 gtaatcgttg aggaactcgc gcgggttgct cccgtccggg tcggtgctgt agtaccgcgg
     2161 gatgacctgc tgataggtgc cagagaggtt ccagcgcggc gtgagcgacg ccagtccgaa
     2221 ctgtgattca gtgaggttgt gcgcaacagc ccctgcttcg aagcagatgc cgtgcgcgcc
     2281 catctgatac cgcgggtaca ccgaaacggc atacatctcg cctgggccgc cggtggccat
     2341 cacgacgttc tcggcgttga agagcgtgag cccaaagccc tcttcttcgg catctttgtg
     2401 gttaaccgcc agcacgccga tgcagcgcct ggccttgcct tcgcccacgg tgaggagagc
     2461 aatcgcttcg tagggcgcga ggatggggat gccgaagcgg cgcacctgcg ccagcgactt
     2521 ctgcaccatg aagcgcgagg tccatggccc ggccgacgtg gcgcgctggc gcggatcatg
     2581 gtcggtcttg tagccgacaa agccgccgcg ctcgttgtgc gggaagggca cgcccttttc
     2641 aacgaggtgg tagaactcgc gcagcgtgtt ctctccctcg atcagggcga ggtcgccgtg
     2701 ggtgcatccg ccctgggtga gagtcagggc aaagtcgtgc ggcgtgtccg gcgtcttccc
     2761 ctgtatgccc agcttgtagt aagtctgctt gtcggagcca gagcagttgg atgcgccggc
     2821 gcccagaccg gcggtgacga tgcagaggag ctcctccggg cgagtctcgc cacgctcctg
     2881 gaaga
//
LOCUS       DAOSCV010000589         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22364_length_2882_cov_2.746728, whole genome shotgun sequence.
ACCESSION   DAOSCV010000589 DAOSCV010000000
VERSION     DAOSCV010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22364_length_2882_cov_2.746728"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="PLY56_13635"
     CDS             <1..1595
                     /locus_tag="PLY56_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ22569.1"
                     /translation="GKHILLVQAENFTASHGSQLSVGGTGTGSKTEFYYAPGTFKPSW
                     AKATDAEIHIFQSGSCRAFKEIVSIQAVDEAARKVTVAGPECVAALHAGDRYFVENVF
                     EELDQPGEWYLDQQEGRLYYWPARPLTPNTVVVAPVVSRMFDFAGDAESGKHVEHIWL
                     RGLTIEATDYQPGDGCIGYGMGKEGVVHLKDARACVVEECTFREIGRYAVCAEGGGDH
                     SICRNEISHTAQGGVLLLGAARNTVSDNHIHHLGWVYKHIGGVVLEGKGTDENVVSHN
                     AIHDSPRYGITLKNAGVRNVIEFNRIERTNTETYDTGGIEVTQHDRNQRSGSVIRNNV
                     VIDTIGYYSVEERPTFLSWSIYLDSFAGGYTVTNNLCIGSSHGGIMLQGGKENRVENN
                     IFVNGRLNQGHISNFASNSEDLVLERNIFYTTSPDATLFNVGRLDERVLRAERNLYYN
                     ASGKEPVFRGGGIPSFEEWQRRGFDQNSVVADPLFVNPRRGNFALRPDSPALKLGFRP
                     FDIKEVGPRHKPCRCQPTPPDR"
     gene            1808..>2882
                     /locus_tag="PLY56_13640"
     CDS             1808..>2882
                     /locus_tag="PLY56_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22570.1"
                     /translation="MMDPKALEQKLDSFDPVARREALGRLAALALPPAPVSENINLHF
                     HSFFSYNAEGWSPSHIAWAAHRAGLYAAGLCDFDVLDGLDEFLQAGELLGLRATVNLE
                     TRAFLKEYADREISSPGEPGVTYIMGGGFARALSAGSPQASGLAAYREAARARNLALL
                     ERINPHVSEIAIDYERDVLPLTPLGVATERHIIRAYLNRSKAVFKTPEATAGYWSGLL
                     GKDAAEIQAILDGPAMEEAVRSKLVKKGGLGYVQPSEESFPPVDDFVVWVASCGAVPM
                     VAWLDGTSEGEKDGRALLECLRAKGCQALNIIPDRNWNIKDPDSRAVKVANLRAIVAA
                     ADDLGMPINIGTEMNKAGLPFVDD"
BASE COUNT          524 a          903 c          995 g          460 t
ORIGIN      
        1 aggggaagca cattctactt gtccaggcgg agaacttcac ggcgagccat gggagccagc
       61 tctctgtcgg tggcacaggc accggctcga agacggagtt ctactacgcc cctggcacct
      121 tcaagccgtc gtgggcgaag gcgacggacg ccgaaatcca catcttccaa tctggcagct
      181 gccgcgcttt caaagagatc gtctccattc aggccgtgga cgaggccgcg cgcaaggtga
      241 ccgtggcggg tccggagtgc gtcgcggcgt tgcacgcggg tgaccgctac ttcgtggaga
      301 acgtcttcga ggagctcgac cagcccggcg agtggtacct cgaccagcag gaaggccggc
      361 tctactactg gccggcccgg ccgctgacgc cgaacaccgt cgtagttgcg ccggtggtga
      421 gccggatgtt cgacttcgcg ggcgatgctg agagcgggaa acacgtggag cacatctggc
      481 tgcgtgggct gacgattgag gccacggact atcagccggg cgacgggtgc atcggctacg
      541 gcatggggaa agagggcgtg gtccacctga aggacgcccg cgcctgcgtc gtcgaggagt
      601 gcaccttccg cgagatcgga cgctacgcgg tctgcgcgga gggcggcggc gatcactcga
      661 tctgccggaa cgagatctcg cacaccgcgc aggggggcgt gctcctcctg ggggcggcgc
      721 gaaacaccgt gtcggacaac cacatccatc acctggggtg ggtctacaag cacatcggcg
      781 gggtggtcct ggagggcaag ggcaccgatg agaacgtggt ctcacacaac gccatccacg
      841 acagcccgcg ctatgggatc acgctgaaga acgccggcgt gcgcaacgtg atcgagttca
      901 accgcataga gcgaacgaac acggagacgt atgacaccgg cgggatcgag gtgacccagc
      961 acgaccggaa ccagcgctcg gggagtgtca tccggaacaa cgtcgtgatc gacaccatcg
     1021 gctactactc ggtggaggag cggcccacct tcctctcctg gagcatctat ctcgactcct
     1081 tcgcgggcgg ctacaccgtg accaataacc tgtgcatcgg gagttcccac ggggggatca
     1141 tgctccaggg gggcaaggag aaccgcgtcg agaacaacat cttcgtaaac ggccggctga
     1201 accaggggca catctcgaat ttcgcgagca actcggagga cctggtgctg gagcgcaaca
     1261 tcttctacac cacatcgccg gatgcgacgc ttttcaacgt gggtcgcctg gacgagcgtg
     1321 tgcttcgcgc cgaacggaat ctctattaca acgcgagtgg gaaagagccg gtgttccgcg
     1381 gcggggggat cccctcattc gaggagtggc agcgaagggg cttcgaccag aacagcgtgg
     1441 tcgcagatcc cctcttcgtc aacccgaggc ggggcaactt cgcgctaagg ccggattcgc
     1501 cggcgctcaa gctcggtttc cgcccgttcg atatcaagga ggtcggcccg cggcacaagc
     1561 cgtgccgctg ccagccgacg ccgccggacc ggtagcgcgc gcatctggct atgctgcgag
     1621 caggcaggga tgccggcagc ttcgcgggcg cccctgcctc tggcgtgacg cccgggcacg
     1681 aatgtggtat gatgagggcg cttgagcccg cgcgcccgag cgcgcgagcg ggccggcgga
     1741 ggctatagca cgagccagca ggagagcggg atgcggcgag aacgcatctc ccacaggaga
     1801 gaaaggcatg atggacccga aggcactgga gcagaagctc gattcgtttg atccggttgc
     1861 gcggcgcgag gcgctgggcc gcttggcggc actggcgttg cctcccgcgc cggtgagcga
     1921 gaacatcaat ctacacttcc actcgttctt ctcctacaac gcggaggggt ggtcaccatc
     1981 gcacatcgcc tgggcggcgc atcgggccgg gctctacgca gcgggcttgt gcgacttcga
     2041 tgttctcgac ggtctcgacg agtttctcca ggcgggcgag ttgctgggtc tgcgcgccac
     2101 ggtgaacctg gagacgcgcg ccttcctgaa ggagtatgcc gatagggaga tctcctcgcc
     2161 gggcgagccg ggcgtcacct acatcatggg cgggggcttc gcccgcgcgc tttccgcggg
     2221 ctctccccag gcgagtggtc ttgccgccta ccgcgaggcc gcgcgcgcgc gtaatctggc
     2281 cctgctggaa cgcatcaacc cccacgtatc cgagatcgcc atcgattacg agcgcgacgt
     2341 gctcccgctg acgccgttgg gcgtcgccac ggagcggcac atcatccgcg cctacctcaa
     2401 ccgctcgaaa gcggtcttca agacgcccga ggccaccgcg gggtattggt ccgggcttct
     2461 cgggaaggat gccgccgaga tccaggcgat cctggacggg ccggcgatgg aggaggcggt
     2521 ccgctcgaag ctcgtgaaga agggcggtct cggctacgtg cagccctccg aggagagttt
     2581 cccgcccgtg gatgacttcg tcgtttgggt cgcctcgtgc ggggccgtgc cgatggtggc
     2641 ctggctggat ggcaccagcg aaggcgagaa ggatggccgg gcgcttctgg aatgcctgcg
     2701 cgccaagggg tgccaggcgc tgaacatcat cccggaccgc aactggaaca tcaaggaccc
     2761 cgattcgcgc gcggtgaagg tggccaacct gcgcgccatc gtcgctgcgg cggacgacct
     2821 ggggatgccg atcaacatcg gcaccgagat gaacaaggcc gggctcccgt tcgtggacga
     2881 cc
//
LOCUS       DAOSCV010000590         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22474_length_2872_cov_3.309549, whole genome shotgun sequence.
ACCESSION   DAOSCV010000590 DAOSCV010000000
VERSION     DAOSCV010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22474_length_2872_cov_3.309549"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="PLY56_13645"
     CDS             <1..1602
                     /locus_tag="PLY56_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22571.1"
                     /translation="PAFDFVLLLPEGVEFVDCVPKGSRNYHKARRIDVGREVRDGVTY
                     QRVVITSDRALGPSRLEKLRFFNGYLGALRATPRLAGEKHRLFYYLQAPGEREREHAL
                     DLVVLPAPEGRQPREIPVGVSLWTIAATDPFWRGLMETYRRCGINSVESGKMGAEPDA
                     ARVAREHGMRPWMLLWWSWWNEEYLKQHPDAAVVKRDGRRDAKMVCPEVVADPRSDAI
                     TAPLAGYVDAAKAGACEVIWWDLEGPGAFEVCFCARCLERFRRENGIAAGEELTPLVI
                     QTRYASQWTAFACAQSARIAARIRQHAREKNAPLRLAVYSGVQNEHTRRDYRVDWTTL
                     APHLDIATPSFYSFGAGGLADQFTRGIRETVKGIKEVHEIPVFATLTTGYEGSSLTRD
                     ARATRMQILKAIAHGADGVYFWWWGTNDGRHYRAIADASRVIAELEPFFLKGTNDDGL
                     VTVEPSAGASHACWRLGNEVAVLLFNHRGDAAQALRVAPGSLPEPGEWRVVRASPDLE
                     KPAWGPGGLSVAVPALDAVWLVLAR"
     gene            complement(1642..2352)
                     /gene="rnc"
                     /locus_tag="PLY56_13650"
     CDS             complement(1642..2352)
                     /gene="rnc"
                     /locus_tag="PLY56_13650"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOJ22572.1"
                     /translation="MHPDVNTLAAEWGITISKPELLAQALVHRSYVREHPEEGQESNE
                     RLEFLGDAILGSLVAEYLFLHFPDFSEGQLSKAKGTAVSEPLLHQAAKAAGLGQHILL
                     SRAEEAAGGRDRPSILSDAFEAVVAAIYLDQGQEAARRFVLRFLADQIDAIARHEHEH
                     DFKTLFQETVQAEGRPTPTYRIVSEEGPDHAKLFTAEARVGRRVLGRGTGRSKKEAEQ
                     SAAHDALDRLAASKARRS"
     gene            complement(2342..>2872)
                     /locus_tag="PLY56_13655"
     CDS             complement(2342..>2872)
                     /locus_tag="PLY56_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22573.1"
                     /translation="AALGAPFVRVFGGRIPPDVSRPDAADDIAQVLTALGGTAASVGV
                     TVVLETHDDFCRGKDAADIIDRVSGPGVGILWDILHPYRCGEALTDTLNYLGGHIRHV
                     HVKDGVNLSPTGFKPVLLGEGEMPWQEAVRLLGQIGYTGYLSLEWEKRWHPEIPDPEI
                     AFPQYMERMREFLHAS"
BASE COUNT          455 a          919 c         1019 g          479 t
ORIGIN      
        1 cctgccttcg acttcgtctt gctcctcccc gagggcgtgg agttcgtcga ttgcgtgccc
       61 aaaggctcgc ggaactacca caaggccagg cggattgacg taggccgcga ggttcgcgac
      121 ggagtgacct accagcgcgt cgtgatcacc tcggaccggg cgctggggcc gtcgcgcctg
      181 gagaagctcc gcttcttcaa cggctacctg ggcgcgctgc gcgccacgcc gcggttggcc
      241 ggcgagaagc accgcctctt ctactatctg caggcgcctg gcgagcgcga gcgcgagcac
      301 gccctcgacc ttgtcgtcct tcccgcgccg gagggaaggc agccgcgcga gatcccagtc
      361 ggcgtctcgc tatggaccat agccgcgacg gatcccttct ggcgcggcct gatggagacc
      421 taccggcggt gcgggatcaa ctcggtggag agcggcaaga tgggagccga gccggacgcc
      481 gcgagagtgg cccgggagca tggcatgcgt ccctggatgc tgctctggtg gtcgtggtgg
      541 aatgaggagt atctgaagca gcatccggat gcggccgtgg tgaagcgtga cgggcggcgc
      601 gatgcgaaga tggtctgccc cgaggtagtc gccgacccgc ggagcgatgc catcaccgcg
      661 ccgctggcag ggtacgtgga tgccgcgaag gcaggggcgt gtgaagtgat ctggtgggat
      721 ctagaagggc cgggcgcctt cgaggtgtgc ttctgtgcgc gctgcctgga gcggttccgg
      781 cgtgagaacg ggatcgccgc cggtgaggag ctgacgccgc tcgtcatcca gacgcgctac
      841 gcgagtcagt ggacggcatt cgcctgcgct cagagcgctc gcattgccgc gcggattcgt
      901 cagcacgcgc gcgagaagaa cgcgccgctt cgcctggcgg tctactccgg ggtgcagaat
      961 gagcacaccc ggcgcgacta ccgcgtggat tggaccaccc tggcgcccca tctcgatatc
     1021 gccacgccaa gcttctactc cttcggcgcg ggggggctgg cagatcagtt cacccgtggc
     1081 attcgcgaga cggtgaaagg gatcaaagag gtgcatgaga tccccgtctt cgccacattg
     1141 accaccgggt atgagggcag ctcgctcacg cgcgacgcgc gggcgaccag gatgcagatc
     1201 ctcaaggcaa ttgctcacgg cgccgatggc gtctacttct ggtggtgggg gacgaacgac
     1261 gggcgccact accgcgccat cgctgatgcc tcgcgcgtga tcgcggagct ggagccgttc
     1321 ttcttgaagg gaacaaacga cgacggcctg gtgacggtgg agccttcggc gggcgcctcg
     1381 cacgcctgct ggcgcctggg caacgaggtg gcggtgttgc tcttcaacca ccgcggcgat
     1441 gcggcccagg cgctgcgcgt ggccccgggc agcctgcctg agccgggcga gtggcgcgtc
     1501 gtccgggctt cgcctgacct ggagaaaccc gcgtggggcc ccggcggtct gtcggtcgcc
     1561 gtgccggcgc tggatgccgt gtggctcgtc ctcgcgcgct gagtctcgcc tggcgggccg
     1621 ggggagatgc caagaggatg gctatgagcg ccgggctttg ctggccgcga gtcggtccag
     1681 ggcgtcatga gcggcagact gctccgcttc cttcttgctt cgccctgtgc cgcgcccgag
     1741 cacccgccgg cccactctcg cctcggcggt gaacagcttg gcatggtccg ggccctcctc
     1801 ggagacgatg cgataagtgg gcgtcggccg gccctcggcc tgcaccgtct cctggaagag
     1861 cgtcttgaag tcgtgctcgt gctcgtgccg ggcaatggcg tcgatctgat cggcgaggaa
     1921 gcgcaacacg aagcggcgcg cggcctcttg gccctggtcc aggtagatgg cggcgaccac
     1981 cgcttcgaag gcatcggaaa ggatcgaggg acggtcgcgg cctccggcag cctcttcggc
     2041 gcgcgagagg aggatatgct ggcccagacc ggcagccttt gccgcctgat gcaggagtgg
     2101 ctcgctgacg gccgtcccct tcgccttgga gagctgcccc tcggagaagt cgggaaagtg
     2161 gagaaagagg tattccgcta ccaggctgcc aaggatggcg tcgccgagga actccaggcg
     2221 ctcgttggac tcctggccct cttcagggtg ctcgcgaacg taggagcggt ggacgagcgc
     2281 ctgggcgagt agctccggtt tcgagatcgt gatgccccac tcggcggcaa gagtgtttac
     2341 atcaggatgc atgcaggaac tcccgcatcc gctccatgta ctggggaaaa gcgatctccg
     2401 gatctggaat ctccgggtgc caccgcttct cccactccag cgagagatag ccggtgtagc
     2461 cgatctggcc caacaggcgc accgcctcct gccacggcat ctctccttcg cccagcaaca
     2521 ccggcttgaa gcctgtcggc gagaggttca ccccatcctt gacgtggacg tgccggatgt
     2581 ggccacccag gtagttgagc gtgtcggtca gcgcctcgcc gcagcggtag gggtggagga
     2641 tatcccacag gatgccgacg ccgggtccgg agacacggtc gatgatatct gccgcgtcct
     2701 tgccccggca gaaatcgtca tgcgtctcca ggacaaccgt cacaccaacg ctcgcggcgg
     2761 tgcctccaag cgcggtgagc acctgggcga tgtcgtcggc ggcatcgggg cgcgacacat
     2821 cgggagggat gcgcccaccg aagacgcgga cgaaaggtgc ccccagggcg gc
//
LOCUS       DAOSCV010000591         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22507_length_2869_cov_3.284648, whole genome shotgun sequence.
ACCESSION   DAOSCV010000591 DAOSCV010000000
VERSION     DAOSCV010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22507_length_2869_cov_3.284648"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="PLY56_13660"
     CDS             complement(<1..2398)
                     /locus_tag="PLY56_13660"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ22574.1"
                     /translation="MNPFEVLNEVRNAYKTFVHTFQQFKNPTIRDWVGEKVDGGTLLW
                     KEPFIELSRRFRRGDSWNDLIGLGIHPETPKVFTAEAGNRTAAPVALYSHQSACIRNV
                     IERRNTIVATGTGSGKSFCFGIPIVSECLRLRQQGMAGIKAVIIYPMNALANSQYEDF
                     AKRLHGSGLKIALYTGDTPDAFPSGEYTTPQEYVKRTAGRAEPYDCELLTRQEIRETP
                     PDILMTNYAQLELLLTRFEDRTLFPPEHAGVLRFLVLDEVHTYTGRRGADVACLIRRL
                     KQHTDTIGKLHCIGTSATVQSGAGEDARQIIADFATRLFGEPFAREDVIREEDHVIPV
                     PEITPEPLPASVLVTRQMVEEFRWETTDEGEPAESTIQQAAVLAEALVGRQLRPAEKT
                     REGLGILLRNHPTLYFLEKRLAEGAAPLSDLLSAYREAHRPGAGVSECLNELMAALLT
                     GTAATVDVYGEQQPRFVPKLHAFFSQGRTITSCLTPQAPHLNDRGETTCPACARKEME
                     RPTFPLAFCRSCGQEFYCVDVQEDGTLAPRDMDALETQGTPAYLSPVPWNADEAPVPE
                     ARHTPRGKVRSNYLPFLPQARTYCPACNKLDSACDCPEKLHVAQLAFPFLFCPSCGVF
                     YDRRVREFTKLFTFGTVGRSTGTDVLISSTLRNLSEGERKIIAFSDNRQDTALQAAHI
                     NSLERRFLFRRGLYQALVEDGHVEGSGSWMGLHEVGRRVLDVFNHYNVTPSFSRSAAG
                     MFAVDPTTEKKYRQYLQYGALEELFRTQNRNQQSLEDVGLLLVVYNGLDRCAESEDFW
                     K"
     gene            complement(2445..>2869)
                     /locus_tag="PLY56_13665"
     CDS             complement(2445..>2869)
                     /locus_tag="PLY56_13665"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HOJ22575.1"
                     /translation="THSDPQKGIRLSALKFELMKRLRKFSQEHIYQHERLRIYRDYAR
                     LIIQSLFKTLLGLYQGRDTLRAVEEKARLYPNLAGGFRDWLWRYSDANPREDSCKLEN
                     EILYRLGEERDYRQAIMDYISGMTDAYAIKAFGELTSF"
BASE COUNT          480 a          892 c          947 g          550 t
ORIGIN      
        1 ccttccagaa gtcttcggat tccgcacacc ggtcgaggcc gttatacacc accagcagca
       61 ggcccacatc ctccaggctc tgctggttac ggttttgcgt gcgaaacagc tcttccagcg
      121 cgccgtactg aaggtattgc cggtactttt tttccgtcgt tgggtcaacc gcaaacatcc
      181 ccgccgccga gcgcgagaag ctcggggtga cgttgtagtg gttgaacacg tccaggacgc
      241 gccggcccac ctcgtggagc cccatccagg atcccgatcc ctcaacgtgc ccatcctcca
      301 cgagcgcctg gtagaggccc cgccggaaga gaaaccgccg ttccaggctg ttgatgtgcg
      361 cggcctgtag cgcggtatcc tggcgattgt cggagaaggc gatgatcttg cgctcgccct
      421 ccgaaaggtt gcgcagcgtg ctcgatatca gcacgtcggt gccggtggag cggcccacgg
      481 tgccgaaggt gaagagtttg gtgaactcac gcacgcggcg gtcgtagaaa acgccgcacg
      541 acgggcagaa gaggaacgga aaggcaagct gcgccacgtg cagcttctcg gggcagtcgc
      601 aggccgagtc cagcttgttg cacgcgggac agtaggtgcg agcctgcggc aggaagggga
      661 ggtagttgct gcgcaccttc cccctggggg tgtgccgggc ttccggaacc ggtgcttcgt
      721 cggcattcca ggggacggga ctgaggtagg cgggagtgcc ttgcgtctcc agcgcatcca
      781 tgtcgcgcgg ggcgagggtg ccatcctctt gcacatccac gcagtagaac tcctgcccgc
      841 aactgcggca aaacgccagg ggaaaggtgg gccgctccat ctccttgcgc gcgcacgccg
      901 ggcaggtcgt ttcgccgcga tcgtttagat ggggtgcctg gggggtgagg cagctggtaa
      961 ttgtgcgccc ttgcgagaag aacgcgtgca gcttgggcac gaagcggggc tgctgctcac
     1021 catagacgtc caccgtcgcg gctgtgcccg tcagcagcgc cgccatcagt tcgttgaggc
     1081 attcgctgac gccggctccg gggcggtgtg cctcgcgata ggcgctgagc agatcggaga
     1141 ggggggcggc accctcggcc aggcgcttct ccaggaagta gagggtggga tggttgcgca
     1201 gcaagatccc gaggccctcg cgggtcttct cggcgggacg gagctgccga cccaccaggg
     1261 cttccgccag caccgcggcc tgctggatcg tggactctgc cggctcgccc tcgtcggtcg
     1321 tctcccagcg gaactcctcc accatctgcc gggtgaccag cacgctcgcg gggaggggtt
     1381 ccggggtgat ctccggtacc gggatgacgt ggtcctcctc gcggatcacg tcctccctgg
     1441 cgaaaggctc cccgaagaga cgcgtggcaa agtcggcgat gatctggcgg gcgtcttccc
     1501 ccgcgccgct ctgcaccgtg gcgctggtgc cgatgcagtg cagcttcccg atggtgtcgg
     1561 tgtgctgctt caggcggcgg atgaggcagg ccacgtccgc gccgcgcctg ccggtgtagg
     1621 tatgcacctc gtccaggacc aggaagcgca gcaccccggc gtgctccggg gggaagaggg
     1681 tgcggtcctc gaagcgggtg agcaacagct ccaactgcgc gtagttggtc atcaggatgt
     1741 ccgggggcgt ctcccggatc tcctggcgcg tcagcagctc gcagtcatac ggttccgccc
     1801 ggcccgccgt ccgcttcacg tactcctgcg gagtggtgta ctcgcccgag gggaaggcgt
     1861 cgggcgtgtc gccggtgtag agcgcgatct tcaaccccga accgtgaagc cgcttcgcga
     1921 agtcctcgta ctggctgttg gcgagggcgt tcatcgggta gatgatgacc gccttgatcc
     1981 ccgccatgcc ctgctgccgc aggcgtaagc attcgctgac gatcgggatg ccgaagcaga
     2041 agctcttgcc ggacccggtg cccgtcgcca cgatggtgtt cctgcgctcg atcacgtttc
     2101 ggatacaggc gctctggtgg ctataaagcg ccaccggcgc tgcggtgcga ttgcccgcct
     2161 cggcggtgaa gaccttcggc gtctccggat ggatgcccag cccaatcagg tcgttccagg
     2221 agtcgccccg ccggaagcgc cgcgagagtt cgatgaacgg ttccttccac aggagcgtgc
     2281 cgccgtctac cttctcgccc acccagtcgc ggatcgtggg gttcttgaac tgctggaagg
     2341 tgtgcacgaa cgtcttgtag gcattgcgga cttcgttcag cacctcaaat ggattcatgg
     2401 ccctctctcc ccgccagtga cgtgccgata aaggttggta cgtattagaa acttgtcagt
     2461 tcaccaaatg ccttgatggc ataggcatcc gtcattcctg agatgtaatc catgatggcc
     2521 tggcggtaat cgcgctcctc gcccaggcgg tagaggatct cgttttctag tttgcaagag
     2581 tcctcgcgcg ggttggcgtc tgaataacgc cagagccagt cccgaaatcc cccggccagg
     2641 ttaggataca gccgcgcctt ctcctccacg gcccgcaaag tgtcgcggcc ctgatacagc
     2701 ccgagaaggg ttttgaacag cgattgaatg atcaggcggg cgtagtcgcg atagatgcga
     2761 agccgctcat gttggtagat gtgctcctgg ctgaactttc gcagcctttt catcaactcg
     2821 aacttcaagg cggagagccg gatacccttc tgcggatcac tatgggtac
//
LOCUS       DAOSCV010000592         2843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22807_length_2843_cov_3.209110, whole genome shotgun sequence.
ACCESSION   DAOSCV010000592 DAOSCV010000000
VERSION     DAOSCV010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22807_length_2843_cov_3.209110"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLY56_13670"
     CDS             <1..1416
                     /locus_tag="PLY56_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22576.1"
                     /translation="GAQQNSAYCKDGYLIDLDLSAGSLTPERVERLHASLASPDRLTL
                     VCANEGKLAELQGALGRAGRSLPSAALCINLSRRAIASPDGWAKVLSGVLDGEFWGGS
                     DDLAPGSPLSWNRRWLGDPGVAERLRRYLLLPYLLGEPITVRETLSFLAYALGGGLSS
                     ESASDLPSHEHLRYRLFNTVFGEPDGHIHGSRSVPNEKLLWWLFRFDPGAQASPGTDL
                     RLLMDLDRLGTVSPPPGDLLAMRQNDLVVRESEKSDTSYRERLARFMRYARRWYALAS
                     EEGFAAYFPFRHFETFTQALNATVEERVKLLPGLVQGLNRLLSGNREDSHKKLYLYHL
                     AGEGSRSSVCIYNTNDPVKASQLSVVTDLELSQEADGPADAYLERFPRRLYLVYDPQP
                     EVRLPISLLLYEVLQSAAGSEGAFPATLWRKERDTVTRFMGALGQAVGADDDEICLTI
                     AGPDKALWELEHTPGEELVVS"
     gene            1413..2804
                     /locus_tag="PLY56_13675"
     CDS             1413..2804
                     /locus_tag="PLY56_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22577.1"
                     /translation="MSTVKMPVTERIYERYRVRREVSQTADLAALFLARLRYEAPEST
                     PGSVQELLGRLAGGQLERVSGARAVAFAHWLRKHLMITETVRGSRKVASYCPIHPALS
                     LATNAEGLRVDAFVRHLAGCFSPEERQRLQERLWDPERQPAFERVVFELLSWQLPPGD
                     PAPPASCPPTLIENAEPGTPDGILLRAKEDLLALSEVVAGVEAFVAHAGRLLAFTVTR
                     YLLACAGVPLTLPIYAAPAADTHEGVKTLAHEIIDYHRGQFQRALAKQFGDAVAAAAG
                     ECGEDPDPADEATAARLAAQLFSGRARIVRPERYAEMRERLGSFTEVALAYYWSESGA
                     TGRFLRQLHTAHLNLAKKAGIANSRSRQSQWHFYWLSPSLVDTLLLASRARFAEDRVL
                     VADLLDEWRERYGVALLIDAGWEAEYRRSFRALGSPDALNEANQRRFSEILSEQGRIH
                     KNSDEFPWVLLRD"
BASE COUNT          452 a          950 c          941 g          500 t
ORIGIN      
        1 ggcgcccagc agaactcggc gtactgcaag gacggctacc tcatcgatct cgacctctcc
       61 gccgggtctc tgacgccgga gcgtgtcgag cgtcttcacg cctcgctggc atcccctgac
      121 cggctgacac tggtctgcgc gaatgagggc aagctcgcgg agctgcaagg tgccctggga
      181 cgtgccggcc ggagccttcc ctccgcggcg ctctgcatca acctcagccg gcgtgccatc
      241 gccagccctg acgggtgggc gaaggtcctc tcaggcgtcc tggacgggga gttctgggga
      301 ggctccgatg acttggcgcc tggttctccc ctctcctgga accgccgatg gctgggcgac
      361 ccgggcgtgg cggagcgtct ccgccggtat ctgctgctgc cgtacctctt gggtgagccg
      421 atcaccgttc gagagaccct ctcctttctg gcctatgctc tcggcggggg cctctcgtct
      481 gagagcgcca gtgacctgcc ctcgcacgag cacctgcgtt accgcctctt caacacagtg
      541 tttggcgagc ccgacggcca tatccatggc agccgttcgg tgccgaatga gaagctcctc
      601 tggtggctct tccgcttcga tcccggcgcc caggcttccc cgggaacgga tctgcgcctg
      661 ctgatggatc tggaccgtct gggtacggtg tcaccgccgc cgggtgatct cctcgcgatg
      721 cggcagaacg acctggtcgt ccgtgagagc gagaagagcg acacctccta tcgggagcgc
      781 ctggcgcgct tcatgcgcta cgctcgccgg tggtacgcgc tcgcgagcga agagggtttc
      841 gccgcctact tccccttccg tcactttgag accttcacgc aggcgctgaa cgccactgtc
      901 gaggagcgag tcaagcttct tcccggcctc gttcagggcc tgaaccggct gctatcgggt
      961 aaccgcgagg acagccacaa gaagctctac ctctaccacc tggcaggcga aggttcccga
     1021 agcagcgtct gcatctacaa caccaacgac ccggtgaagg cgtcgcaact cagcgtggtg
     1081 accgatctgg agctgagcca ggaggccgac ggcccggccg atgcctacct ggagcgcttc
     1141 ccgaggcggc tctacctcgt ctatgacccg caaccggagg tccgcttgcc catctccctg
     1201 ctgctctatg aggtactgca gagcgcggcc ggcagcgagg gagcgttccc cgctaccttg
     1261 tggcgcaagg agcgcgatac cgtcacccgg ttcatgggag cactcggcca ggcggtcggc
     1321 gcagacgatg atgagatctg cttgacgatt gccggccccg acaaagcact gtgggagctt
     1381 gagcacaccc ctggcgagga gttggtcgta tcatgagcac cgtgaagatg cccgtgacgg
     1441 agcgcatcta cgagaggtac cgcgtgcggc gcgaggtgag ccagacggcg gacctggcag
     1501 cgctcttcct ggctcggctt cgctacgagg ccccagagag cacgcctgga tcggtgcagg
     1561 aactgctcgg acgcctcgcc ggtgggcagc ttgagcgcgt atcgggtgca cgagcggttg
     1621 ccttcgccca ctggctccgg aagcacctca tgatcacgga gacggttcgc ggcagccgga
     1681 aggttgcatc gtactgcccc atccacccag ccctctccct ggcgacaaat gccgagggcc
     1741 tgcgcgtgga cgcgttcgtg cgacaccttg ccgggtgctt cagcccggag gagcgccagc
     1801 ggcttcagga gaggctctgg gatccagagc gtcagcctgc cttcgagcgg gtggtcttcg
     1861 agttgctctc gtggcagttg cccccagggg atccggctcc tcccgcgagt tgcccgccga
     1921 cactgatcga gaacgccgaa ccgggcacgc cggacggcat cctcctgcgc gcgaaggagg
     1981 acctgctggc gctctccgag gtcgtcgcgg gcgtcgaggc gttcgtggct catgccggcc
     2041 gcctgctggc cttcacggtg acgcgatacc tgctggcttg cgccggcgtg ccactcacct
     2101 tgccgatcta cgcagcccct gccgcggaca cacatgaggg ggtgaagacc ctcgcccacg
     2161 agatcatcga ctaccatcgc gggcagttcc agcgggcgtt ggcgaagcag ttcggtgacg
     2221 cagtcgccgc ggccgctggt gagtgcgggg aggatcccga cccggccgat gaggcgacgg
     2281 ctgcgcggct ggccgcgcag ctcttctcag ggcgggcccg catcgtccgc ccggaacgat
     2341 acgcggagat gcgggagcgg ctcggcagct tcaccgaggt agccctcgcg tactactggt
     2401 cggagagcgg cgcgacaggg cggtttctcc gccaactcca taccgcccac ctcaaccttg
     2461 cgaagaaggc cggcatcgcg aactcacgga gccggcaaag ccagtggcac ttctactggc
     2521 tgtcgccgtc gctggtcgac acgctgctgc tggcatcgcg cgcgcgcttc gctgaagacc
     2581 gggttctcgt cgcagacctc ttggacgagt ggcgggaacg ctacggggtt gcgcttctga
     2641 tcgacgccgg gtgggaggcg gaataccgcc gctcgttccg agccctggga agccctgacg
     2701 cgctgaatga ggccaatcag cgccgcttct ccgagatcct ctccgagcag ggccggatac
     2761 acaagaactc ggatgagttc ccgtgggtgc ttctgaggga ctgacgcatg aatacgaagg
     2821 aattgctggt agacgtgctg gtc
//
LOCUS       DAOSCV010000593         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22891_length_2835_cov_4.397482, whole genome shotgun sequence.
ACCESSION   DAOSCV010000593 DAOSCV010000000
VERSION     DAOSCV010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22891_length_2835_cov_4.397482"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /locus_tag="PLY56_13680"
     CDS             <1..1419
                     /locus_tag="PLY56_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22578.1"
                     /translation="DAAQRLKVTFAGLPGSPKPEWIRVRQTVWTPAKPSSRAPEGVEQ
                     PRVAGSALPDAPDGVVTVPGGTTGQVWLSLDTSAVPAGDYRGRVELTPASGEPLTVPI
                     RVRVSALSMPNELTLALGGWDYTDGPGRGITEENLEATVEFLRSYHVNAPWAVSAVMP
                     FGKHDATGKMVEPPDTKRLDAWIARWPNARYYCVYNAFTKDVETPEDRRRVAEWITFY
                     VEHLKKKGIAPSQLVLLLVDEPRNEEQDQRIVSYARVLREVAPEVIVFNDPIWKDPRD
                     TSREMLELSGMLCPNRVSWLGSRGPYEEVYLPQRDAGRLLAFYSCSGPVRALDPYSYH
                     RLQAWEAFRYGAIYQAFWAFGDTGGGSAWNEYSAPGTCYSPQFLDASGNTTSKHMEAI
                     REGLYDYEYLVLLRKAVEQAEKSGRNPAAVRQARQLLTTLPAKVVDAEGAQAIDWHAP
                     KQRDVADNARKEVLKALEALSQ"
     gene            complement(1513..>2835)
                     /locus_tag="PLY56_13685"
     CDS             complement(1513..>2835)
                     /locus_tag="PLY56_13685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOJ22579.1"
                     /translation="MRALGAHSAMLVPMEVRGRVLGAINIVSTRSARRYTHRDLTQAM
                     ELGRRAALAIENARLYREAQDANRAKDHFITVISHELRNPLAPILAGVDMLRHLVTED
                     GRLRRTLEIIDRNARLQARLVNDLLDLSRVARGKIELRCVPVALDTIVRSAIQGQQSD
                     LADARLSLHVDLQSGLWVNGDADRLQQVVMNLLSNAIKFTPAGGEIRVSCRAQDPGHA
                     RIVVEDTGIGIAPEQLPRLFRMFEQGKMDKHKGAGMGIGLALVKALVERHGGSVTAES
                     EGPGKGSRFTVELPLIPAPETASADNRLQQAPDHLRVLLVEGDPDTREILVTALGIVG
                     YSVTSAPSGEEALERAREERPGLILADVDLPGMDGYEFLKRARALPGMQSVPALMLTG
                     DQKAIDVQRTREAGFPAHLTKPVDPDALEARIRELVQAGPQARTEGKS"
BASE COUNT          461 a          945 c          934 g          495 t
ORIGIN      
        1 gacgcggcgc agcgcctgaa agtcacgttc gctgggctcc ctggttcgcc gaagccggag
       61 tggatccggg tgcgccagac agtatggact ccggccaagc cgtcgagtcg ggcccccgag
      121 ggcgtcgagc agccgcgcgt ggcgggctct gcgctccccg acgcccctga tggagtggtt
      181 acggtccctg gtggtaccac cggacaggtg tggctgagcc tcgacacctc ggcggtgccg
      241 gcgggcgact atcggggccg ggtggagctg actcccgcct cgggcgagcc gctcacggtg
      301 cccattcgtg tgcgtgtctc ggctctctcc atgcccaacg agctgaccct cgcccttggg
      361 ggatgggact acaccgatgg ccccgggcgc gggatcaccg aggaaaacct ggaggcaacc
      421 gtcgagtttc tgcgctccta ccatgtgaac gcgccgtggg ccgtgagcgc ggtgatgccg
      481 ttcggcaagc atgatgccac cggcaagatg gtcgagccgc cggatacgaa gcggctggat
      541 gcctggatcg cgcgctggcc gaacgcgcgc tactactgcg tctacaacgc cttcaccaag
      601 gatgtggaga ctcccgagga ccggcggcgc gtcgccgagt ggatcacctt ctacgtggag
      661 catctgaaga agaaggggat cgctccctcg cagctcgtgc tccttctcgt ggatgagcct
      721 cgcaacgagg agcaggatca gcgcatcgtg agctacgcgc gcgtcttgcg cgaggtggcc
      781 ccggaggtga tcgtcttcaa cgatccgatc tggaaggacc cgcgagacac cagtcgagag
      841 atgctggagc tttctggcat gctctgcccc aatcgcgtct cctggcttgg gagtcgcggc
      901 ccctacgagg aggtctacct tccccaacgc gatgccggac ggctgcttgc cttctatagc
      961 tgctccggac cggtgcgagc gctcgatccc tacagctacc accgcctcca ggcgtgggag
     1021 gcgttccgct atggcgcgat ctaccaggcg ttctgggcct tcggcgatac cggcggcggc
     1081 tccgcgtgga acgagtactc cgcgcctggc acctgctact cgccgcagtt cctggatgcc
     1141 tcggggaaca ccacctcgaa gcatatggaa gcgatccgcg agggcctgta tgactacgag
     1201 tacctggttc tcctgcgcaa ggcggtcgag caggccgaaa agagcgggcg caatcccgcc
     1261 gccgtgcgcc aagcacgcca gctactgaca acgcttcccg ccaaggtggt ggatgccgag
     1321 ggagctcagg cgatcgattg gcacgcgccg aagcagcgcg atgtggcgga caacgcccgc
     1381 aaagaagtgc tgaaagcact ggaagctctc tctcagtagg aagcacgcag gggggcgggc
     1441 tgaggtccgc ccctctctct aagctcgcca gacgcgcatc ttggggaaag ccgatctcct
     1501 gatgcgctcc gctcaggatt tgccctcggt gcgggcctgc gggcccgctt gcaccagttc
     1561 gcggattcgt gcttcgaggg catccgggtc caccggcttt gtcagatgcg cggggaatcc
     1621 cgcctcgcgt gtgcgctgga catcgatcgc cttctgatcc cccgtgagca tcagggccgg
     1681 cacgctctgc atcccgggca gggcgcgagc gcgcttcagg aactcatatc catccatgcc
     1741 aggcaggtcg acgtctgcca ggatgaggcc ggggcgctct tcccgcgccc gttcgagggc
     1801 ttcctcgccg cttggggctg acgtgacgga gtagccgacg ataccgagtg ctgtcaccag
     1861 gatctcgcgc gtgtctggat ccccttcgac caacagcacg cgcaggtgat ccggcgcctg
     1921 ctgcagccta ttgtctgcgg acgccgtctc cggtgcgggg atgaggggga gctctaccgt
     1981 gaagcggctt ccctttcccg gaccttcact ctcggccgtg acgcttcctc catgcctctc
     2041 cacgagcgcc ttcaccaggg ccaggccgat ccccattccg gcacccttgt gcttgtccat
     2101 tttcccctgc tcgaacatgc ggaagaggcg gggaagctgc tccggcgcga tgccgatccc
     2161 ggtatcctca accacgatcc gcgcgtgccc gggatcctgc gcccggcacg acacccggat
     2221 ttcgccccca gccggagtga acttgatcgc gttggagagg agattcatca ccacttgctg
     2281 cagccggtcg gcgtcgccgt tcacccagag cccgctctgc agatcgacgt gcagtgacag
     2341 ccgggcatcc gccagatcgg attgctggcc ctgaattgcc gagcgcacga tggtgtcgag
     2401 ggcaaccggg acgcagcgaa gctcgatctt gccacgtgcc acccgcgaga gatccagcag
     2461 gtcattgacc aggcgcgcct gcaggcgggc gttgcggtcg atgatctcga gggtgcggcg
     2521 caaccgccca tcctcggtca caaggtggcg cagcatatcg acgcctgcca ggatcggggc
     2581 gagggggttg cgcaactcgt gcgaaataac cgtgatgaag tgatccttgg cgcggttggc
     2641 atcctgtgcc tcacgataga ggcgcgcgtt ctcgatggcc agggccgcac ggcggcccag
     2701 ctccatggcc tgcgtcaggt cgcggtgcgt ataccgtctc gccgaccggg tggagacgat
     2761 gttgatcgcg ccgagtaccc gtccccgcac ctccatcggc accagcatcg ccgagtgcgc
     2821 gccaagtgcc cgcat
//
LOCUS       DAOSCV010000594         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22925_length_2832_cov_2.911775, whole genome shotgun sequence.
ACCESSION   DAOSCV010000594 DAOSCV010000000
VERSION     DAOSCV010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22925_length_2832_cov_2.911775"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLY56_13690"
     CDS             <1..202
                     /locus_tag="PLY56_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22580.1"
                     /translation="QIIDLLIRLVKSMGLPTTTYTDLYREYAAAPEKVPGLDAWRWDD
                     ELDNGPLRLPQSLERRGGERVS"
     gene            complement(197..793)
                     /locus_tag="PLY56_13695"
     CDS             complement(197..793)
                     /locus_tag="PLY56_13695"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOJ22581.1"
                     /translation="MRPDWNRWVRRGRWALLGSLAYLGLNVLEVVLGSYRDRTGMADC
                     AIILGAAVWPGERPSLTLRARTWRGGEVYRAGYTRHLILTGSVGKHPPAEAEVMRRLL
                     TAWGVPAEAMVLEDQAKTTAESARLCAEIMRRRGWESALIVSDPWHTPRAARLFRREG
                     IRALPCPAFDSPTWTRLGKRLYYTLREAAILASRRFLS"
     gene            complement(899..1123)
                     /locus_tag="PLY56_13700"
     CDS             complement(899..1123)
                     /locus_tag="PLY56_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22582.1"
                     /translation="MWQSRVTPLSAGACLHFVGDGALTVTDIELSEEFANLFVLDEGG
                     GHVSLFIAPYDGTPITIRRWITFGYRLKAE"
     gene            complement(1105..>2832)
                     /locus_tag="PLY56_13705"
     CDS             complement(1105..>2832)
                     /locus_tag="PLY56_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22583.1"
                     /translation="GFEPFLRALLAARKAHPALTRGEADYLAVETSAPEVCGILRALP
                     DAAAIVAINFSSRPVVARLAIEESRLPGKVATGPWHDALGAQVKVRRTGTRLCLRVPL
                     APYGTAVVVPGAPSPIPTPVASPSRAAESSEGRIEHRNPHYRLLIDRSTGLLQALEAA
                     DGTTLLSAMAIRTDPRDLQGGGDRVEWQARPQPRFRERSRGCKRVLEFSGTVSRGPED
                     ETPILHYTLAYTLDATPKVAVELRLMAVEAIEEVPALVQTLRLPAGGWWFVETLEGEM
                     CGETVARHTEEEPRFTARYRHSQPWALWEERLLPLAGRFGARLPGGPGVALGGVGASM
                     PGWRQNLVLRAPALGEEGVGCQAELRWGDGCEALSLRAGQCISLRFDAVLLPAGEGAP
                     PARPRARVPILIVEGPRYTVENAHYRATLVRSRGGGLVELRAPGGASLLRDSAIYSDR
                     GLYGDETDPDGHRVPIAATTQGDTEPEMVIEREEGQLIITVDSTLHRPDGGDGAVLRP
                     ITRARLCYTFDDGPEIGVTLSLLPPGVTAEPVFLAQRMQLAGVQYCTPVTSSSPRRSR
                     ARIACGNRE"
BASE COUNT          470 a         1007 c          929 g          426 t
ORIGIN      
        1 ccaaattatt gatctgttga tccggctggt gaagtcgatg ggcctgccca cgaccaccta
       61 caccgacctg taccgcgagt acgccgcggc gcctgagaag gtgcccggac tcgatgcctg
      121 gcgctgggac gacgagctgg acaacggtcc cctccgcctt ccgcaatcgc tggagaggcg
      181 tggcggcgag cgtgtttcat gagaggaagc gccggctcgc caggatcgct gcctcccgga
      241 gagtgtagta gaggcgcttt ccaagacgcg tccaggtggg gctatcaaac gcggggcaag
      301 ggagcgcgcg gattccctcg cgccggaaaa gccgcgcagc gcgcggcgtg tgccagggat
      361 cgctcacaat cagcgcgctc tcccacccgc gccggcgcat gatctccgcg cacaggcgtg
      421 cgctctcggc ggtggtcttc gcctgatcct cgaggaccat cgcctcggcg ggcacacccc
      481 acgcggtgag gaggcggcgc atcacctcgg cctccgcggg agggtgcttc cctacgcttc
      541 ctgtcaggat aaggtggcgt gtgtagccgg cccggtagac ttcaccaccg cgccaggtgc
      601 gcgcccgcag cgtcagcgag gggcgttctc ccggccacac tgcggcaccc aggatgatgg
      661 cacaatcggc catgccggtg cggtcgcgat aggagccgag caccacctcg agcacgttga
      721 gcccgagata ggcgagcgat cccagaagtg cccatcggcc tcgccgcacc cacctgttcc
      781 agtcaggtct catctactcg ccacccttca ttccaccgcg ccaccacgcc agcggcgagc
      841 gtgtctcgcc gtgctggagg acgaagatcc cttcgagggg agtggcacca gccctctcct
      901 actctgcttt caggcgatag ccgaaggtaa tccagcgccg gatggttatc ggcgtgccat
      961 catagggcgc gatgaagagg ctgacgtggc ccccgccctc gtcgaggacg aagaggttgg
     1021 cgaactcctc gctcagttcg atgtccgtca ccgtgagcgc gccatcgccc acgaagtgga
     1081 ggcaggcgcc cgccgatagc ggcgtcactc gcgattgcca cacgcgatcc gcgccctgct
     1141 ccgacgcggg gaagaactgg tcaccggcgt acagtactgg acgccggcca gctgcatgcg
     1201 ctgtgccagg aagaccggct ccgcggtcac gcccggcggc aggagagaga gggtgacacc
     1261 tatctccggg ccatcatcaa aggtgtagca aagacgcgcc cgggtgattg gccgtagcac
     1321 ggcgccgtca cctccatcag ggcgatggag cgtgctgtca accgtgatga tcaactgccc
     1381 ctcttcccgc tcgatcacca tctccggctc ggtatcccct tgcgtggtag cggcgatcgg
     1441 cacgcggtgg ccgtccgggt cggtctcatc gccgtagaga ccccggtcgc tatagatcgc
     1501 gctatcgcgc aacagcgatg cacctccagg cgcgcgcaac tccaccaaac ctccgccacg
     1561 gctacgcacc agggtggccc ggtagtgggc gttctccacg gtatagcgtg gtccttcgac
     1621 gataaggatg ggaacgcgcg cacgagggcg agccggcggc gctccctctc ccgccggcag
     1681 gagcaccgca tcgaaacgga gagagatgca ctgccctgcc cggagcgaga gggcctcgca
     1741 gccatccccc cagcgcagtt ccgcctggca cccaacgccc tcctcgccga gcgccggcgc
     1801 ccgcaggacc aggttctgcc gccagccggg catgctggcg cctacgccgc cgagggccac
     1861 gcccggcccg ccggggaggc gtgccccaaa gcgtcctgcg aggggaagga ggcgctcttc
     1921 ccagagggcc catggctgcg agtgccggta tcgcgccgtg aagcggggct cctcctccgt
     1981 gtgacgggct accgtctcgc cgcacatctc cccctccagg gtttccacaa accaccaccc
     2041 gcccgccggg agccgcagcg tctgcaccag cgcgggtacc tcctcaatcg cctcgaccgc
     2101 catcaagcga agctctaccg ctaccttcgg ggttgcgtcc agggtgtagg ccagggtgta
     2161 gtggagaatc ggtgtctcgt cttcagggcc tcgtgaaacc gtaccggaaa actccagcac
     2221 gcgcttgcat cccctactcc gctcgcgaaa gcggggctgc gggcgggcct gccactccac
     2281 ccggtcgccg ccaccctgga ggtcgcgcgg atccgtccgg atcgccatcg cggagaggag
     2341 tgtcgtgcca tcggccgcct ccagcgcctg caacaacccg gtgctgcggt cgatgagcag
     2401 gcggtagtgc gggttccgat gctcaattct cccctcggag ctctctgccg cccgcgaggg
     2461 actggccacg ggcgtcggaa tgggagaagg tgcgcccggc accacaaccg ccgttccata
     2521 gggcgccaga ggcacgcgca agcacaggcg cgtgcccgta cgccgcacct tcacctgggc
     2581 ccccaaggca tcgtgccagg ggcccgtcgc caccttcccc ggcagtcttg actcctcgat
     2641 agccagccgc gccacgacgg gccggctgct gaagttgatg gcgacaatgg cggcggcatc
     2701 cggaagcgcg cgaaggatgc cgcacacctc gggagcgctg gtctccacgg ctaggtagtc
     2761 cgcctcgccc cgggtgagcg cggggtgggc tttgcgcgcg gcgagaagcg cgcgcaggaa
     2821 cggctcgaaa cc
//
LOCUS       DAOSCV010000595         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22930_length_2831_cov_3.235231, whole genome shotgun sequence.
ACCESSION   DAOSCV010000595 DAOSCV010000000
VERSION     DAOSCV010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22930_length_2831_cov_3.235231"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLY56_13710"
     CDS             complement(<1..293)
                     /locus_tag="PLY56_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22584.1"
                     /translation="MLDQVKVGFIGAGGNASGHMQRVAEIEGARITAVCDLVPEKAEA
                     AAAKYGATAYTDYRRLLDEADLNAVYVSVPPFAHYDAEILAAGYPRDAVDRVVR"
     gene            complement(377..1405)
                     /locus_tag="PLY56_13715"
     CDS             complement(377..1405)
                     /locus_tag="PLY56_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOJ22585.1"
                     /translation="MLNWVQPRVDSAELAEALPREFEGLPADLCLLPGAGTGNRSFRA
                     VPGPSDGERTGWFVRVRNPKYAAEDAMRFEHDLLTHLKAAGLPVYPPLLSRSGEPWAW
                     IGNACVQLSPLVAGEAYAAGNVEQIAAAARFLSRLHREGRRFLGDPRKQWDREDSFMI
                     AFAGFDLVRNRDTSGRYTADLNAVAASIHRFMRELPPQRFWKLPQSVVHGDFHQGNLL
                     FKGDRLAGVFDWDYACEHPRLKDIANALMFLCARLPRPLDAADIRSYVQPMRFEPEWV
                     DAFFAAYEEAEPLTAEEWIALPAMMVGRWLQIRSCAIIKLPEEERLDVFCDGMAETLD
                     RLWSFSVR"
     gene            complement(1459..>2831)
                     /locus_tag="PLY56_13720"
     CDS             complement(1459..>2831)
                     /locus_tag="PLY56_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ22586.1"
                     /translation="RANVIIEPKLEGLGAEDYEAAAALIDAGYEAAAARAAELRRYAL
                     KAPEWKAHLAQRRARRRADGFTPRFLQLAGGCVATCRAVTENLRPFIGRPLNVSELET
                     ALMALAGQGRIEGLLYEKAETGGEEGLMIFIQEKDYGPPFIHALIDVNGADTDAVQFN
                     VPMRLSMLDFQGSGAELRADVTLGSRTRLAGEYYRPLGRRQWFIAPRALYRREATSWS
                     PLGAYSTRELGLGLDIGWSYGRTSELRLGVWSGHLRVSPRGESTVPLQDGAHSFLSAR
                     WTTDLQDSAVIPTRGTRFHVDGRWYFHTPGAAPGFPALQADVTRFSPLGPHETLFLFG
                     SVGVTFTATAPLASRLETISPLRLGLGLDTQPFGSHHLLGGIGYLRGISRLPAPVWRT
                     VYLGVWYARGGVFESWSNARLVGSLGGGVLAETIAGPVFLGTSWSGGTQRIYFSVGRF
                     LKSTAL"
BASE COUNT          479 a         1010 c          915 g          427 t
ORIGIN      
        1 cgcaccacac ggtcgaccgc atcgcgcgga taaccggccg ccaggatctc ggcgtcgtag
       61 tgcgcgaacg ggggcacgct aacatagacg gcgttgagat ccgcctcgtc cagcaggcgc
      121 cggtagtcgg tgtaggcggt cgccccgtac ttcgccgcgg cggcctcggc cttttcggga
      181 accaggtcgc aaaccgccgt aatccgcgca ccctcgatct cggcgactcg ctgcatgtgc
      241 ccgctggcgt tgccgccggc gccaataaag cctactttca cctgatccaa catcgttctc
      301 ctccagcgct ctcggggatg cctctcggga gcatcccctc tctgcgtctc tcccgcgccg
      361 ttcaggagct agctcgctac cgaaccgaga agctccacag ccggtctagc gtctctgcca
      421 tgccgtcgca gaaaacgtcg agacgctcct cctcggggag cttgatgatc gcgcagctcc
      481 ggatctgcag ccagcgcccc accatcatcg cggggagcgc gatccactcc tcggcggtga
      541 ggggctcggc ttcctcatac gccgcgaaaa acgcatccac ccactccggc tcgaagcgca
      601 tcggctggac gtaggaccgg atatccgccg cgtccagcgg gcggggcagg cgcgcgcaga
      661 ggaacatcag cgcgttggcg atatccttca ggcgcgggtg ctcgcacgcg tagtcccagt
      721 cgaagacgcc cgccagacgg tcacccttga agagcaggtt cccctggtgg aaatcgccgt
      781 gaacgacgct ctgcggcagc ttccagaagc gctgcggagg cagctcgcgc atgaaccggt
      841 ggatgctcgc cgccaccgcg ttcaggtcgg cggtataccg cccggacgtg tcgcggttgc
      901 gcaccaggtc gaacccggcg aaggcgatca tgaagctgtc ttcccgatcc cactgcttgc
      961 gcgggtcgcc caggaagcgc cggccctcgc ggtggaggcg cgagaggaac cgcgccgccg
     1021 cagcgatctg ctccacgttc ccggcggcat acgcctcccc cgcgacgagc ggggagagct
     1081 gcacgcacgc gttcccgatc caggcccacg gctcgccgct cctcgaaaga agaggcgggt
     1141 acaccggcag cccggcggct ttcaggtgcg tcaggaggtc gtgctcgaag cgcatcgcgt
     1201 cttcggcggc atacttcggg ttgcgcacgc gcacgaacca cccggtgcgt tcgccatccg
     1261 acggaccagg aactgcccgg aacgagcggt tgcccgtgcc cgcccccggc aggaggcaca
     1321 gatcggcggg aagtccctca aactcgcgcg ggagcgcctc ggccagctcc gcgctatcta
     1381 ctcttggctg gacccagttc aacatcacac tcctcagttt ccgccgcgcc cggagaactc
     1441 ctcttcgcgc acgaccgttc agagcgccgt ggatttgagg aaacgcccga cggagaagta
     1501 gatccgctgc gtgccgccgg accagctggt gccgaggaag accgggcccg cgatcgtctc
     1561 cgccaaaacg cctccgccga ggctgcccac caggcgggcg ttgctccagc tctcgaagac
     1621 gccccctcgg gcataccaca cccccagata gaccgtgcgc cagacgggcg ccgggaggcg
     1681 cgagataccg cggaggtagc cgatacctcc caggaggtgg tggctcccga acggctgcgt
     1741 gtccagccca agccccaggc gcagcgggct gatggtctcg agccggctgg ccagcggcgc
     1801 cgtggccgtg aacgtcacac ctaccgaccc gaagaggaaa agcgtctcgt gcgggccgag
     1861 gggggagaag cgcgttacat cggcctggag tgccgggaag cccggcgcgg cgcccggcgt
     1921 gtggaaatac caacgaccat ctacatgaaa gcgcgtgcct cgcgtgggga tcaccgcgct
     1981 atcctggagg tccgtcgtcc agcgcgccga gaggaagctg tgcgcgccat cctggagcgg
     2041 aaccgtgctc tctcctcggg gagagacgcg caggtgtcca ctccatacgc ccaggcggag
     2101 ctcgctcgtg cgcccgtagc tccagccgat atccaggccc aggccaagct cgcgcgtcga
     2161 gtaggctcca aggggcgacc aggaggtcgc ctctcgccgg tagagggcgc gcggggcgat
     2221 gaaccactgc cggcggccaa gcgggcgata gtactccccc gccaagcggg tccgcgagcc
     2281 gagcgtcaca tcggcgcgta gctccgcgcc cgagccctgg aagtcgagca tggagaggcg
     2341 catcggcacg ttgaactgca cggcgtcggt atcggcgccg ttgacatcga tcaacgcatg
     2401 gatgaaggga gggccgtaat ccttctcctg gatgaagatc atcagaccct cttcccctcc
     2461 ggtttccgcc ttctcgtaga gcaggccctc gatgcgtcct tgacccgcaa gggccatcag
     2521 cgctgtctcg agttctgaaa cgttcaacgg gcggccgatg aacgggcgga ggttctccgt
     2581 caccgcgcgg cacgtggcca cacatcctcc cgcgagctgc aggaaccggg gagtgaagcc
     2641 atccgcgcgc ctccgcgccc ggcgctgggc cagatgcgcc ttccattcgg gcgctttcag
     2701 cgcatagcgc cgcaactcag cagcccgcgc cgccgctgcc tcgtagcccg cgtcgatgag
     2761 cgccgccgca gcctcgtagt cttccgcgcc cagaccctcc agcttgggct cgataatgac
     2821 gttcgcccgc t
//
LOCUS       DAOSCV010000596         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22965_length_2828_cov_3.300397, whole genome shotgun sequence.
ACCESSION   DAOSCV010000596 DAOSCV010000000
VERSION     DAOSCV010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22965_length_2828_cov_3.300397"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..2547)
                     /locus_tag="PLY56_13725"
     CDS             complement(37..2547)
                     /locus_tag="PLY56_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ22587.1"
                     /translation="MAEEKWRVPPERLAWRCDPDTFGFETTEDIEPLKGIAGQERAVE
                     SIAFGIDIDAPGYNLFLVGAPGTGRWHAVRTQIEERARTEPRPPDWCYVHNFDNPDEP
                     VALCLPAGGGEEFAAAMDNLIALSQEEIPKAFQSDGYEELKSQLIREHNERRDRLIEE
                     LQAKAREAGFVIQMTPAGMVTIPLVNGQPITPEQYEELPEEQKEEIEERGKELPGQIE
                     QTFRQARNIEREALEKSRELDQSVARQAVSQLFDAVREKYRGNEKIERFLNAAQNDIA
                     EHVDEFRNGREHLPSDSPPALAVAANMAEMQREETLNRYRVNVLVNNAHTEGAPVVAE
                     EHPTYYNLVGRIDFRVRFGVMVTDPRMIKAGALHRANGGYLIVDLIDVLSNPLSWDGL
                     KRTLRTNEVRIENLGEQMGIIPTASLRPEPIPVQLKVVLIGPPFLYYLLHALDKDFGR
                     LFKVKVDFDTEMPRNENHPAQYTALVAALTRKEGMKPFSKGAMARAVEHAARTREHQE
                     KLATSYRVAEDLVSEASFWAGKAGHDVVMAEDVTKALEEREYRSRMLEEKIREWIEEG
                     TLMIDTTGAVVGQVNGLSVSALGDYVFGRPSRITGRTFLGRGGVVNIEREVKMGGRIH
                     NKGVLILAGYLGGRYAREVPLALSATLTFEQLYEDVEGDSASAAELYALLSSLADVPI
                     RQGIAVTGSVNQRGEIQPIGGATHKIEGFFHVCKVRGLTGEQGVIIPEQNVRNLMLKD
                     EVIEAVKEGKFHIWAVRHIDEGIEILTGMPAGRQYPDGSFEEGSINDRVLWRLRHFAE
                     TLKEFGVQPGPEKEAEEALAGVAETLEETDDPSEEGGR"
BASE COUNT          444 a          988 c          845 g          551 t
ORIGIN      
        1 agaagcactc acgcgcctcc cgccccggcc cgataactac cgaccgccct cctcggaggg
       61 gtcgtcggtc tcttctagtg tttccgccac tccggcgagg gcctcttcag cctctttctc
      121 ggggcccggc tgcactccga actccttgag cgtctcggca aagtggcgca accgccacag
      181 cacccggtcg ttgatcgagc cttcctcgaa gctaccgtcc gggtactgcc ggccggcggg
      241 catccccgtc aggatctcta tcccctcatc gatgtggcgc actgcccaga tgtggaactt
      301 cccctccttg accgcctcga ttacctcgtc cttgagcatg aggttccgga cgttctgctc
      361 cgggatgatc acgccctgtt ccccggtgag cccgcgcacc ttgcagacgt ggaagaagcc
      421 ctcgatcttg tgcgtggccc ctccgatcgg ctggatctcc ccccgctggt tcaccgagcc
      481 tgtgacggca atcccctggc gaatcggcac atcggccaga ctcgatagga gggcgtaaag
      541 ctccgccgcc gaggcgctgt cgccctcaac atcctcatac agctgctcga aggtgagtgt
      601 ggccgagagc gccaggggca cctcgcgggc ataccggccc cccagatagc cggcgaggat
      661 caggacgccc ttgttgtgga tgcgcccgcc catcttcacc tcgcgctcga tgttcaccac
      721 gccaccgcgc cccaggaagg tccgccccgt gatccgcgag ggccggccga agacgtaatc
      781 gcccagcgcc gagaccgaca gcccattgac ctggccgaca acggccccgg tggtgtcgat
      841 catcagcgtg ccctcttcga tccactcgcg aatcttctcc tcgagcatgc gcgagcgata
      901 ctcccgctcc tccaacgcct tggtgacgtc ctctgccatc accacgtcgt ggccggcctt
      961 gcccgcccag aaggaggcct cggagaccaa atcctcggcg acacggtagg aggtggcgag
     1021 cttctcctgg tgctcccggg tgcgcgcagc gtgctcgacg gcgcgtgcca tcgctccctt
     1081 ggagaacggc ttcatcccct ccttgcgtgt taacgctgcg acgagcgccg tgtactgcgc
     1141 cggatggttc tcgttgcggg gcatctcggt gtcgaaatcg accttcacct tgaagaggcg
     1201 accgaaatcc ttgtcgagcg cgtgcagcag gtagtagagg aaggggggac cgatcaggac
     1261 caccttgagc tgcacaggga ttggctccgg gcgcagcgag gccgtgggga tgatccccat
     1321 ctgctcgccc aagttctcga tgcgcacctc gttggtgcgc agggtgcgct tcaggccatc
     1381 ccaggagagc ggattgctga gcacgtcgat cagatccacg atcaggtagc cgccgttggc
     1441 gcgatgcagc gcccccgcct tgatcatgcg tggatcggtg accatgacgc cgaagcgcac
     1501 gcgaaagtcg atccgcccga cgaggttgta gtaggtgggg tgctcctcgg ccaccacggg
     1561 cgccccctcg gtgtgggcgt tgttgacgag cacgttgacc cggtagcgat tgagtgtctc
     1621 ctcacgctgc atctccgcca tgttggcggc caccgccagg gcaggggggc tatccgacgg
     1681 caggtgttcg cggccgtttc ggaactcgtc cacatgctcg gcgatgtcat tctgagccgc
     1741 gttcaggaag cgctcgatct tctcgttgcc ccggtacttc tcgcgaacgg catcaaacag
     1801 ttggctcacc gcctggcgcg cgacgctttg gtccagctcg cggctcttct ccagcgcctc
     1861 gcgctcgata ttgcgcgcct gccggaacgt ctgctcgatc tggcctggca gttccttgcc
     1921 gcgctcctcg atctcctcct tctgctcctc gggcagctcc tcatactgct ccggggtgat
     1981 cggctggcca ttcaccaggg ggatcgtcac catgcccgcc ggcgtcatct ggatgacaaa
     2041 gccggcttca cgcgccttgg cctgcagctc ctcaatcagg cggtctcggc gctcgttatg
     2101 ctcccggatg agctggctct tcagctcctc atagccgtcc gattggaaag ccttcgggat
     2161 ctcctcctga cttaaggcga tcaagttgtc catcgcggca gcgaactcct cgccgccgcc
     2221 cgctggcagg cacagcgcca cgggctcatc cggattgtcg aagttgtgga catagcacca
     2281 atccggaggc cggggctcgg tgcgcgcgcg ctcctcgatc tgcgtgcgca ccgcgtgcca
     2341 cctgccggtg cctggggcac cgaccaggaa gaggttgtag ccgggcgcat cgatgtcgat
     2401 gccgaaggcg atagactcga ccgcgcgctc ctggccggcg atgcctttca ggggctcgat
     2461 atcctctgtc gtttcgaacc cgaacgtatc tggatcacac cgccacgcca atctctccgg
     2521 cggcactcgc cacttttctt ccgccatacc ccctccctcc tggtatcttg gccattccag
     2581 cgccttcacg ttttttgccc ggaagccacg acgccaaact tgccggcccg ccgcggtccc
     2641 gtcactgcgg gggagccaag gaagccctcg atgcgttgac cgtcttcccc agctaggaga
     2701 cgtatgctat aatgggcatg acatgaaatt ggcgtcggag gccagaagaa acgtgtcgga
     2761 tgcgcaacgg ttctgctcaa gcgcgttctg gggcgcccct ccggcacgaa ggctccccct
     2821 cagacggg
//
LOCUS       DAOSCV010000597         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22978_length_2826_cov_3.701552, whole genome shotgun sequence.
ACCESSION   DAOSCV010000597 DAOSCV010000000
VERSION     DAOSCV010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22978_length_2826_cov_3.701552"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLY56_13730"
     CDS             complement(<1..188)
                     /locus_tag="PLY56_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ22588.1"
                     /translation="MDFSVSSKDATLANGQRAVITKVVGKVDVYTAPRLKDKLLQLER
                     EGIHHIAVDLTEVGFIDSV"
     gene            complement(827..1933)
                     /gene="mnmA"
                     /locus_tag="PLY56_13735"
     CDS             complement(827..1933)
                     /gene="mnmA"
                     /locus_tag="PLY56_13735"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOJ22589.1"
                     /translation="MSGREGRGRRVLVAMSGGVDSSVAAALLVEQGYDVIGATMRLWS
                     GASPEADERSCCSLSAVEDARRVAAHLGIPYYVLDFEAVFEERVVRDFVREYASGRTP
                     NPCIRCNQHLKFAALLERARQLGADYIATGHYARVGFDEETGRYRLRRGVDRSKDQSY
                     ALYMLTQEQLAAALFPLGEWTKDQTRRRAAELGLGVARKRDSQEICFVPGDDYGSFLR
                     EHQPALAVPGKIVDRSGRVLGEHPGVCFFTIGQKRGLRLPLPYPMYVVEIIAETNTLV
                     VGSEAELMRRSCIVEAVNWIAVPPLAGELPVMAKVRYNMAEQPAVVRELPGGRAHLEF
                     AQAQRAITPGQAAVFYHDEDVVGGGTVSAAGLRG"
     gene            complement(2044..>2826)
                     /locus_tag="PLY56_13740"
     CDS             complement(2044..>2826)
                     /locus_tag="PLY56_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOJ22590.1"
                     /translation="LGGLPVEMAPEALAHLADMAGGDGRTALNALEVAVMLASPDEDG
                     VRRVTLALVEEAMQQRAIIYDRDGDSHYDVISAYIKSMRGSDPDAALYWLARMIAAGE
                     DPLFIARRLVIQAAEDIGNADPMALVVATAAAQAVQLVGMPEGRIPLAEATIYLATAP
                     KSNASYVAISRALADVEERGPAPVPLHLRDASYRGARAMGHGKGYRYPHDYPGGWVDQ
                     EYVPEGTLRRRYYEPTDRGREARIRERLEALEKAKREARRGT"
BASE COUNT          485 a         1083 c          780 g          478 t
ORIGIN      
        1 acgctatcga tgaagcctac ctcggtgagg tcgacagcga tatggtgtat cccctcgcgc
       61 tccagttgga gcagcttgtc cttcagacgg ggcgccgtgt atacatccac cttgccgacc
      121 accttggtga tgacagctcg ctgtccgttg gcgagagtag catccttgct cgaaacacta
      181 aaatccattg gtctcccccc aggtggtcac ctatagcacg tgccgcccac cactcgtccg
      241 ccctccaggg cgggcacgcg cgacaactga cgcactggac ttggtcttcc gccttcctag
      301 gccgactcgg cacctgtgct gggccgctcc cacttcgcag tgtactcggc aaactggtgc
      361 gtaagctgat cgaggcgttg ccgaagttcg tcaatgcggt gcgacgcatg gtgttccgcg
      421 ctgcggctca gacgcaagta gagccagact cctgcgcctc ccagaactgc cgcgcccagg
      481 agaatcccca gccccccggg gacgcaaaca ccacaggctg cggagtctcc ttcgggggag
      541 tcggcccttg cctcgagggc actgcccgcg gagtgccggt cgcacgcctg cagctgcccc
      601 tgttccaccc tgggcccgct ccccgtcacg cggctctcgg ccaccgtact cttggcgccg
      661 ccaggcgcca cacttccacc caccacttcc gccgggggat ctgcaccgtc gctcacggcg
      721 ttgcctccca tccctctccg gcgcccagcg tctctgaccc agacctggca ggctcgctgc
      781 aggcctccgg gacagccctc aggcgccttt tgttttaccc actttcctat ccgcgcaaac
      841 cggcggcact caccgttccg ccacccacca catcctcatc gtggtagaag accgccgcct
      901 gccctggggt aatcgcgcgc tgtgcctgcg caaactccaa atgcgccctc cctccaggca
      961 gctctctgac caccgccggc tgctccgcca tattataccg aactttcgcc atcacgggca
     1021 attcgcctgc caagggcggc accgcgatcc agttcaccgc ttccacgata caggatcgcc
     1081 gcatcaactc ggcctccgac ccaaccacca acgtgttcgt ctccgcgatg atctccacaa
     1141 catacatcgg gtatggcagc ggcagtctaa ggcctcgctt ctggccaatc gtgaagaagc
     1201 agacccctgg gtgctccccc aatacgcggc cgctccggtc cacaatcttt cccggcaccg
     1261 ccagcgcggg ctggtgctcg cgaaggaagc tgccgtagtc gtcacccggc acaaaacaga
     1321 tctcctggct atcgcgcttc cgggccaccc ccaaacccag ctccgccgcg cgtcgccgcg
     1381 tctgatcctt ggtccactcg cccagcggga agagcgcggc ggcaagctgt tcctgcgtga
     1441 gcatgtagag ggcataggac tgatccttgc tccgatccac tccccggcgc agccgatacc
     1501 gcccggtctc ctcatcaaag cccacgcgcg cgtagtggcc cgtggcgata taatcggccc
     1561 ccaactgccg cgcccgctcc aagagtgccg cgaacttcag gtgctggtta cagcggatgc
     1621 acggattcgg agtgcgcccg ctggcgtatt cgcgcacgaa gtcgcgcacc acgcgctcct
     1681 caaaaaccgc ctcaaaatca agcacataat aggggatccc aaggtgcgcc gcaacccggc
     1741 gcgcatcttc gacggccgac agcgagcaac acgaacgctc gtccgcctcg ggcgaggcac
     1801 cagaccagag ccgcatcgtc gccccgatca cgtcataacc ctgctccact aggagcgcgg
     1861 ccgccaccga gctgtccacg ccgccgctca tggcgacaag cacgcgccgc cctctgccct
     1921 cacggcccga catggcctga atgtgcaacg tcacctcctc tcgcgcgaaa tacaaccctc
     1981 acatacgcaa cccacgggcc agcaccccct tttcatcggg gaaggccggc catcgctcat
     2041 acctcacgtg ccacgcctgg cttctcgctt ggccttctcg agcgcctcaa gtcgctcgcg
     2101 gattcgggct tcgcggcccc gatccgtggg ttcgtaatac cgccggcgca aggtgccctc
     2161 cggcacatac tcctggtcca cccaaccacc agggtagtca tgcggatagc ggtagccctt
     2221 gccgtggccc atcgccctgg cgccacggta gctggcatcg cgcaggtgca gcggcaccgg
     2281 cgcgggcccc ctctcctcca catccgccag cgcgcgactg atcgctacat agctcgcatt
     2341 cgacttcggc gccgtggcca ggtagatcgt cgcctcggcg agtggaatcc gaccctcggg
     2401 cattccgacc aactgcaccg cctgggcagc cgccgtcgcc accaccagcg ccatcgggtc
     2461 cgcgttcccg atatcctcgg ccgcctggat caccaggcgg cgcgcgatga agagcgggtc
     2521 ctcgcccgcg gcgatcattc gggcgagcca ataaagcgcg gcgtcgggat ccgagccgcg
     2581 catactcttg atgtaggccg agatgacatc atagtgcgag tcaccgtcgc ggtcgtagat
     2641 gatcgcccgc tgctgcatcg cctcctcgac cagggccagc gtcacccggc gcaccccgtc
     2701 ctcatcgggg gaagcgagca ttacggccac ctccagggca ttgagcgccg tacgcccatc
     2761 cccgccggcc atgtcggcca ggtgggccag ggcttctggc gccatctcca ccggcaggcc
     2821 ccccaa
//
LOCUS       DAOSCV010000598         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_22981_length_2826_cov_3.288344, whole genome shotgun sequence.
ACCESSION   DAOSCV010000598 DAOSCV010000000
VERSION     DAOSCV010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22981_length_2826_cov_3.288344"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..2748)
                     /locus_tag="PLY56_13745"
     CDS             complement(55..2748)
                     /locus_tag="PLY56_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22591.1"
                     /translation="MSRLGLKRGWAVLAAAGAFLMATMAPGRAQTTTDPLLANAQALV
                     LQNQYIRLAYGISGTPAVAVRAAPDTSFTGRWGIDTTGGDPSTALDDNRIAFAWNFLS
                     YQIEPQLEAQSDRWYRIGNTQLGSYTIGPDRDLALGRITSRWSSGGTIAVTVSSGSGS
                     QTVQQPPYVDVEETLTLVRDMVRAEYRFINTGSLARRVRVRFYMDPAEGGVWTVGRQQ
                     LEVETDFRGGQVPVDWRMYCPVATPELVLRGIFNRFGATLPNRVVVGDAARLASGPWN
                     YTVNPSIRLSGEPDPQEDAAQAYYETFDLMPYQSKTFVMYVGLGVTDGDPTPPYVLAV
                     QSPRCLGFTLGDDPDTPERETSYVSPRSFTVQAFMYNVAEFDIQNASVFITLPAGLRL
                     AAGESPVQQIGTILRQREGVASWRVEVEPGVSGVLSYTVTSSGMPVSAKTATGSIEIP
                     AQATRAFKAGLSMMSVPFQFANPDVAMALNMSPDRIRLARWDSKARKYAFYPDSSVST
                     IEPGMGFWFKPDLDMTLALKDASPVPRSMSANFILPIKQGWNMIGAPYVYAVPWANTR
                     VSDGQTVISLEEAARAGWLRPTLFRYNPNLSPPGYEFDSLLDAMLEPWQGYWVRSDRD
                     LFLLIPKVTAPYASIVAPDQRSGQPAGWRIQLAARTRSASDLFNFAGASPSATDGYDL
                     GDVDEPPVPSEGFVSLSFVHNDWGRHNGEYTQDLRSSDFGLEKSWDVAVRTQGDEEVT
                     LTWPDLNARLPRGLTATLEDTTTGRRIFMRTQAAYTFRSQAGSVRRLKLVVSRRANAP
                     LAITNVRMQAVPGRAGARAISFDASSDSVVDVRILSPTGGVVSEVTSRAPACAGHNSV
                     VWDGTNRSGALLTSGLVLVEVSARTPDGTAVRVVSPCVLVR"
BASE COUNT          512 a          964 c          895 g          455 t
ORIGIN      
        1 gtgcccccaa cagggagcgg cggttgcctg cgaagttcgg gggctcgccc cacgctacct
       61 gaccaggacg cacggcgata ccacacgaac cgccgtgccg tccggggtcc tcgccgacac
      121 ctccaccaac acgagaccgg aggtgaggag cgccccggaa cggttcgtcc catcccagac
      181 cacgctgttg tgacccgcgc acgccggtgc ccgcgaggtc acctcactga caacgccacc
      241 ggtgggcgat aggatgcgca catccaccac gctatcgctc gacgcatcga aactgatcgc
      301 gcgcgcgccg gcgcgtccgg gcaccgcctg catccgcacg ttggtgatcg ctagcggcgc
      361 gttcgcccgg cgcgatacca ccagcttcag cctgcgcacg cttcccgcct gcgaccggaa
      421 ggtgtaggcg gcctgagtgc gcatgaagat gcgccgccct gtcgtggtgt cttccaacgt
      481 ggcggtgagg ccacgaggca gacgagcgtt gagatccggc caggtgagcg tgacctcttc
      541 atcgccttgg gtacgcacgg ccacatccca actcttctcc agtccgaagt cgctgctgcg
      601 gagatcctga gtatattcgc cgttgtgccg gccccaatcg ttgtgcacga acgagagtga
      661 cacaaacccc tcgctgggca ccggcggctc gtccacgtcg cccaggtcat acccgtccgt
      721 ggcggacgga gacgcgccag cgaagttgaa cagatccgat gccgaccgcg tgcgcgccgc
      781 caactggata cgccacccag ccggctgccc ggagcgctgg tccggcgcga cgatggaagc
      841 gtacggcgcg gtcaccttgg ggatcaggag gaacagatcg cgatccgagc gcacccaata
      901 cccctgccat ggctccagca tcgcgtccag gagactatca aactcgtaac ctggcggcga
      961 caggttgggg ttgtaccgga agagcgtcgg ccgcagccac ccagcgcgcg ccgcctcctc
     1021 aagcgagata accgtctgcc catcggacac gcgcgtgttc gcccagggca cggcgtagac
     1081 atatggagcg ccgatcatgt tccatccctg cttgatcggc aggatgaagt tggcggacat
     1141 gctcctcggc accgggcttg catccttcag cgcgagcgtc atgtccaggt ccggcttgaa
     1201 ccagaacccc atccctggct cgatggtgga gaccgaggaa tcggggtaga aggcgtactt
     1261 ccgtgccttt gaatcccagc gtgccagtcg gatccggtca ggcgacatgt tcagcgccat
     1321 ggccacgtcc ggattcgcga actggaacgg cacgctcatc atcgaaagcc ccgccttgaa
     1381 ggcgcgcgtt gcctgcgccg ggatttcgat actcccggtc gctgtcttcg cgctcacggg
     1441 cattcccgaa gaagtgacgg tataagagag aacaccagac acccccggct cgacttcaac
     1501 tcgccagctg gccactccct ctcgctggcg caggatggtg ccgatctgtt gcaccgggga
     1561 ctcgccggcg gcaaggcgca gccctgccgg aagcgtgata aagacactgg cgttctgaat
     1621 gtcgaactcg gcgacattgt acatgaacgc ctgaaccgtg aagctgcggg gcgacacgta
     1681 ggaggtctcc cgctcgggcg tgtccggatc atcaccgagg gtgaatccaa gacaacgcgg
     1741 cgactgcacg gccagaacgt acggcggcgt cggatcgcca tccgtcaccc cgaggcccac
     1801 atacatcacg aacgtcttcg actgatacgg catcaggtcg aacgtctcgt agtaggcttg
     1861 agccgcgtcc tcttgcggat ccggctcgcc ggagagccgg atgctcgggt ttaccgtata
     1921 gttccaggga ccagatgcca ggcgggccgc atcaccgacg acaacacggt tggggagcgt
     1981 tgccccgaac cggttgaaga tgccgcgcag caccagctcg ggcgtggcga cggggcagta
     2041 cattcgccag tccacgggaa cctgcccgcc gcggaaatcc gtctcgacct ccagctgctg
     2101 gcggcccaca gtccagacgc cgccctcggc ggggtccata tagaagcgga cccgcacccg
     2161 gcgcgccagg ctgcctgtat tgatgaaccg gtactccgcg cgcaccatgt cgcgcacgag
     2221 cgtgagcgtc tcctccacgt cgacgtacgg cggctgctgg acggtctgcg acccgctgcc
     2281 agaggagacg gtcacggcga tggtgccgcc gctgctccag cgtgaagtga tccgcccgag
     2341 ggcgaggtcg cggtcaggcc cgatggtgta cgagccgagc tgcgtgttcc cgatgcgata
     2401 ccaccgatcg gattgtgcct ccagctgggg ctcgatctgg taactgagga agttccacgc
     2461 gaaggcgatc cgattgtcat cgagcgccgt ggaggggtca ccgccggtgg tgtcaatccc
     2521 ccaacggcct gtgaagctgg tatcgggcgc ggcgcgaaca gccacggcgg gcgttccgct
     2581 tatcccatag gccagacgga tgtactggtt ttgcagaacg agcgcctgcg cgttagcaag
     2641 gagcgggtcc gtcgtcgtct gcgcgcgccc tggggccatc gtggccataa ggaacgcgcc
     2701 agccgccgcc agcaccgccc atcctcgttt taagccaagt cgactcacag tcgccctctc
     2761 ctccgtgggc cgcgcgccat ccgatgccgc gaccacccac accacacccg gggacgcacc
     2821 cttggg
//
LOCUS       DAOSCV010000599         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23001_length_2825_cov_2.509025, whole genome shotgun sequence.
ACCESSION   DAOSCV010000599 DAOSCV010000000
VERSION     DAOSCV010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23001_length_2825_cov_2.509025"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..866
                     /gene="lepB"
                     /locus_tag="PLY56_13750"
     CDS             105..866
                     /gene="lepB"
                     /locus_tag="PLY56_13750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ22592.1"
                     /translation="MWILGLPRRSTLGCTALWHRFLGKRGDSLRARLIPLLTLLICLG
                     GVWRWSPFRLGVVCGHSMHPTLRNGSLFLIERGYYQSHPIRRGDVIVFRTKKDACVKR
                     VYAIGGDLICVLVAPETPTVLIDPERYPALVDTLSRRQHAAGTPRIFRVPPHTVFVLG
                     DNIGVSRDSRHFGLVAETQVIGRVLGVGHGRPAPRGVILRGTGPVPAHPHPSGPGPMG
                     PVYHAGGALRRSPIPSATLSGGKGTRQRKRGPGYG"
     gene            complement(857..1573)
                     /locus_tag="PLY56_13755"
     CDS             complement(857..1573)
                     /locus_tag="PLY56_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ22593.1"
                     /translation="MSNISGKAILLTGATSGIGRALALRLSSEGARLAVCGRSPEKMA
                     SLLDAITTAGGRPPLHATFDLASEQEILAFIERANADLGCLDILINNAGVNITKAPVS
                     EIQTDHFDAMLAVNLRAPMIFMREAFRLMSARGEGHVVNILSSACLYSNETMGAYTAG
                     KAGLDALTRIFRKECQPHGIRVTAIYPGGTNTPFRAKPRPDYMAAESVAEAIVGLLNL
                     PRDVVPHEFVFRPEVEVNYP"
     gene            complement(1679..2713)
                     /locus_tag="PLY56_13760"
     CDS             complement(1679..2713)
                     /locus_tag="PLY56_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOJ22594.1"
                     /translation="MNIPPADGVRVMPEELRRFAREVLEKVGVPEEHAVLLANTLVET
                     DQRGVLSHGTQQLLRYTRQFRAGELNPDPQVRVSQEAPATAVVDGDGGLGHFAAYRAV
                     EVALAKAKEMGTAAVVAVNHGHIGAAGTYTRRVLPEGCAALCVSGVDTRSHRPIGPPR
                     HILQVGAGGPISFAIPASAEPPLVLDMGCYFSHRPARTRDREARLQAMREHLDEVFDV
                     APDAVFKIMGLGAVTMALGGILAGVSLPENPDDDRLYPGANQGTFLAVFDVARFMPLD
                     TFLAEMDQFVRKTRSLEPLPGCAEAELPGGPEWRRERDWATEGIPIGGRHREMLEEVA
                     QECGVELPLP"
BASE COUNT          456 a         1018 c          880 g          471 t
ORIGIN      
        1 agtgtctgac tgccctggcc agggccttcc gactggcgtg agaggcatcc tcctggatgc
       61 cccaccattc gagtgcatca cttcgttctt ggagggtgtt ccggatgtgg attctgggtc
      121 tgccgcgaag aagtaccctt ggttgcacgg cactctggca ccggttcctc ggcaagaggg
      181 gcgactcgct gagagcacgc ctgatcccgc tgctcacgct tctgatctgt ctgggcggcg
      241 tctggcgatg gagtccgttc cgcctgggcg tcgtctgcgg tcactccatg caccctaccc
      301 tgcggaacgg gagcctcttc ctgatcgagc gaggctacta tcagagccac ccgatccgaa
      361 ggggcgacgt gatcgtcttc cggacgaaga aggacgcatg cgtcaagcgg gtctacgcca
      421 tcggggggga tctgatctgc gtgctcgtgg ctccagaaac gcccacggtt ctgatcgacc
      481 cggagcgcta tccggccttg gtcgataccc tcagccgcag gcagcatgcg gccggcactc
      541 cgcgcatctt ccgtgtcccc ccgcacacgg tgtttgtcct gggcgataac atcggggtct
      601 cccgcgatag caggcacttc gggctggtcg cggagacaca ggtgatcggg cgcgtcctgg
      661 gcgtcggcca cgggcgccct gcgccaaggg gcgtgatcct gcgcggcacc ggaccggtgc
      721 cagcccaccc gcacccatca ggaccgggcc cgatgggccc ggtctatcac gcagggggcg
      781 cgctccggag gtcgcccatt ccatcggcca ccctgtctgg cggaaagggc acccggcagc
      841 gaaagcgcgg gccgggctac gggtagttca cctcgacctc ggggcgaaaa acaaactcgt
      901 ggggcaccac gtcgcgcggc aggttcaaga ggccgacaat cgcctcggcg acgctttccg
      961 cggccatgta gtcaggccgc ggcttggcgc ggaacggagt gttggtcccg cccgggtaga
     1021 tcgcggtcac ccggatccca tgcggctggc attccttgcg gaagatacgc gtgagcgcgt
     1081 cgaggccggc cttgccagcc gtgtaggcgc ccatcgtttc attcgaatag aggcaagccg
     1141 aggagaggat gttcaccacg tggccttcgc cacgcgcgct catcagccgg aacgcctcgc
     1201 gcatgaagat catcggtgcc cgcagattca cggcaagcat ggcgtcgaag tggtcagtct
     1261 ggatctcgct cacaggcgcc ttggtgatgt tcaccccggc gttgttgatg aggatatcga
     1321 ggcaccccag atcggcgttc gcgcgctcga tgaacgccag gatctcttgc tcgctcgcca
     1381 gatcgaaagt ggcgtgcagg ggcggcctcc cgcccgccgt cgtgatggca tcgagtagcg
     1441 acgccatctt ttcgggggag cgcccgcata cagcgaggcg cgccccctcg ctcgagagcc
     1501 gcagcgccag cgcccggcca atgccagacg tggctcccgt caacaggatc gccttgccgg
     1561 aaatgttact cattttcccc ttcctggtat tcgcgacgtc tggtgccgcg gcgaacagga
     1621 gcaccgcttg tgccagggag agcattggac gccacgcgcc cactcccgct ttgcgcggct
     1681 aaggcaaggg cagctctacc ccgcactcct gggccacctc ttccagcatc tcgcgatgcc
     1741 tcccgccaat cggaatcccc tcggtagccc aatcgcgctc tcgccgccac tccggaccgc
     1801 ccggcagctc cgcctcggca cacccgggca acggctccag agagcgcgtc ttgcgcacga
     1861 actggtccat ctccgccaga aacgtatcga gcggcatgaa gcgcgcgacg tcgaacaccg
     1921 cgaggaacgt cccctggttc gctcccggat agaggcggtc atcatcggga ttctccggca
     1981 ggctgacccc cgccaggatt ccccccagcg ccatggtaac cgcgcccagc cccatgatct
     2041 tgaagacggc atccggagct acatcgaaga cctcgtccag atgctcgcgc atcgcctgca
     2101 ggcgcgcttc tctgtcgcgc gtgcgcgcgg gccggtggga gaagtagcag cccatgtcga
     2161 gcaccagcgg cggctcggcg ctcgcgggaa tcgcgaagct gatcggcccg cccgcgccca
     2221 cctgcaggat gtggcggggg ggcccaatcg gccggtgcga tcgcgtgtcg acgccagaga
     2281 cgcacagggc ggcgcacccc tcgggcagca cccggcgcgt gtaggttcct gccgcgccaa
     2341 tgtgcccgtg gttgaccgcc acaaccgccg ccgtgcccat ctctttcgcc tttgccaagg
     2401 ccacttccac cgcgcgatac gccgcaaaat gccccaggcc gccatcgcca tcgacgaccg
     2461 cggtggccgg cgcctcttgc gaaacacgca cctgcggatc tggattgagc tccccggcgc
     2521 ggaactgccg ggtgtagcgg agcagctgtt gcgtgccgtg gctcagaacg cccctctgat
     2581 ccgtctccac cagggtgttc gccaacagca ccgcgtgctc ctccggcacc cccaccttct
     2641 caagcacctc acgcgcgaac cggcgcaact cctccggcat gacgcgcacc ccatcggctg
     2701 gcggtatgtt cacccctaac ttctcctact ccacagacac tctgctccca tcacgacgcg
     2761 cgcccggccc caggccaatc cgccggcgcc cctctcgcgc cggcacaagc gcgcgccccc
     2821 ccctc
//
LOCUS       DAOSCV010000600         2818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23081_length_2818_cov_3.201592, whole genome shotgun sequence.
ACCESSION   DAOSCV010000600 DAOSCV010000000
VERSION     DAOSCV010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23081_length_2818_cov_3.201592"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLY56_13765"
     CDS             complement(<1..189)
                     /locus_tag="PLY56_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOJ22595.1"
                     /translation="MLIDTNTYLGHWAFRPLPWRDADSLLRAMDAHGIDQAFVSSASA
                     ILYRNTQAGNEELFAQVRG"
     gene            complement(183..965)
                     /locus_tag="PLY56_13770"
     CDS             complement(183..965)
                     /locus_tag="PLY56_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ22596.1"
                     /translation="MVIDCHAHLSGPKNDVTISWGVPDEKVIADSERLGIDAMCVSLL
                     TRERPATPDGFRECNRLLAEITRRYPGRVLGYAYVNPGYQREAIDEVRRCIEEYGFIG
                     VKLYNEYKCTDPVVWPIVEEAVALRVPILHHAGHSHYFVATQPNMSDGGELAELARRY
                     PEATIICGHVGGGGDWEWTIKAVRPAPNVYLDTSGSVFDAGMIDMAVRILGPERLLFG
                     CDLSYTAGVGKIRAAALDDEARRLVLGGNMQRILAMRGGATC"
     gene            complement(1283..2695)
                     /locus_tag="PLY56_13775"
     CDS             complement(1283..2695)
                     /locus_tag="PLY56_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22597.1"
                     /translation="MMPVVQLLAGLTAIGWGPGAWPVWNASPGIPAGAAPAQVARVEG
                     IRVPLPTPIVLPGPRIWTPGFAPVPLASMPDFWPSYRKVPPGQAGMGYYHNPAAPFAR
                     RAYSEESDPVLQQPDQNRMQEVIATGSAPIGDGTYQEARNRAVQQALRTAVEQVTGVY
                     LSSTTITEKFAVLNERIYLRSEGFAVPGDVLEESVTQGVLTLRMRVRVSLRPLMDSLR
                     QLGLTRKWRVAVAIRDEPSGLEGAARLADAEITRRLVDAGFQVVRPAGLEHLDLASLP
                     HSLGTSGGVPAVDVLLAGTAALHLAARAPVTLEGRVIAVIPIYQARIHARAIRVETGE
                     VVTVRHTEELVSDRVDALAATAAVEVASRRAARLFLEDILTLPASHTRRVRLEVDGFE
                     KRGDAQAFQDALALFPGVRGIHRPEYADGLLTLEVELEADAAERLGTDLEQAPGFREF
                     ALTVEADTKARIRARARSSP"
BASE COUNT          444 a          971 c          958 g          445 t
ORIGIN      
        1 gccccgcacc tgggcgaaaa gctcctcgtt gccggcctgc gtgttgcggt agaggatggc
       61 actggccgag gagacgaacg cctggtcgat gccgtgggca tccatggcgc gcaacaggct
      121 gtcggcgtcg cgccagggca ggggccggaa agcccagtgg ccgaggtagg tgttggtgtc
      181 gatcagcatg tggctcctcc gcgcatcgcc aggatccgct gcatgttgcc gcccaggacc
      241 agccggcggg cctcgtcgtc gagcgcggca gcgcggatct tgcccacgcc tgcggtgtag
      301 gagaggtcgc acccgaacag cagccgctcc ggaccaagaa tgcgcacggc catgtcgatc
      361 atcccggcgt cgaagacgct gccgctggtg tcgaggtaga cgttgggcgc ggggcgaacc
      421 gccttgatcg tccactccca atcgccgccg ccgcccacgt gcccgcagat gatcgtggct
      481 tccggatatc ggcgtgccag ctccgccagc tcgccgccgt ccgacatgtt cggttgggtg
      541 gccacgaagt agtggctgtg cccggcgtgg tgaaggatcg gcacgcgcaa cgcgaccgcc
      601 tcttccacga tgggccagac gaccgggtcg gtgcatttgt actcgttgta cagcttgacc
      661 ccgatgaagc cgtactcctc gatgcagcgg cgcacctcgt caatcgcctc gcgctggtag
      721 ccggggttga cgtaggcgta accgagcacg cgcccgggat agcgccgggt gatctcggca
      781 aggagacggt tgcactcgcg gaagccatcc ggcgtggccg ggcgctcgcg cgtcagcagc
      841 gagacgcaca tcgcgtcgat ccccaggcgc tcggagtcgg caatcacctt ctcgtccggg
      901 acgccccagg agatcgttac atcatttttc gggcctgata ggtgagcgtg acagtcaatg
      961 accatgcgtg gctccaggcc ggcctgctgt ttcgggtggg tgtagcgccc gcgcggccgg
     1021 cagctatgga accagttagg gggtggggcg cggaatcctc cagtgccttt atgaagattg
     1081 gtccactcgc gagcggaatg gggacagagc cctgaacgag gagggaaggc accggtgccc
     1141 ggcaggagct tcccaggcag aatgggccgg ggcgcggagc gcgccccggc ttctcgcacg
     1201 gtctgtcttg ccttcctgct ctcctaccac acctgcctgc ctcgtgccac ccacgcgtcc
     1261 tcccgctgcc tgccgtgcga gttcacggag agctgcgcgc ccgcgcccgg attcgggcct
     1321 tggtatccgc ttcgaccgtc agggcgaact ccctaaatcc gggcgcctgc tccaggtcgg
     1381 tgccgaggcg ttcggctgca tctgcttcga gctctacctc aagcgtgagc aggccatcgg
     1441 cgtattccgg gcgatggatc ccgcgcacac cggggaagag ggcgagggca tcctggaacg
     1501 cctgggcgtc cccgcgcttc tcaaaaccat ccacttcgag gcgcactcgc cgcgtgtggc
     1561 tcgcgggcaa agtgaggata tcctccagga acagacgcgc cgcgcgccgt gacgccacct
     1621 ccaccgccgc cgtcgccgcg agcgcatcca cgcggtcgga gaccagctcc tcggtatggc
     1681 gcaccgtgac cacttcgccc gtctccacgc gaatggcacg agcatggatc cgtgcctgat
     1741 agatcgggat gacggcgatc acccggcctt ccagcgtgac gggcgcgcgc gccgccaggt
     1801 ggagggccgc cgtgccggca aggagcacat cgaccgccgg cacgcccccg ctcgtaccga
     1861 gcgagtgggg cagcgaggcg agatccaggt gttccaggcc cgcaggccgc accacctgga
     1921 agccagcatc gaccaggcgc cgcgtgatct ctgcgtctgc caatcgggct gctccctcca
     1981 ggcccgaggg ctcatcacga atcgccacgg ccacgcgcca cttccgcgtg agcccgagct
     2041 ggcgcagaga gtccatcagc ggtcggaggc tgacacgcac gcgcatccgg agcgtgagga
     2101 cgccttgggt gacgctctcc tcgaggacgt cgccgggcac ggcgaagccc tcgctgcgca
     2161 ggtagatccg ctcgttcaga accgcaaact tctcggtgat cgtggtgctg gagaggtaga
     2221 ccccggtgac ctgctccacg gcggtgcgca acgcctgttg gacggcgcga ttccgggcct
     2281 cctggtaggt gccatcgccg atgggcgcgc tgccggtggc gatcacctcc tgcatccggt
     2341 tctgatccgg ctgttgcagc accggatcgc tctcttccga gtaggcccgg cgagcgaaag
     2401 gcgccgcggg attgtgatag tagcccatgc cggcctgtcc ggggggcacc tttcgatagg
     2461 acggccagaa gtcgggcatg gatgcgagcg gcacgggcgc gaaaccgggc gtccagatgc
     2521 gcggccccgg caggacgatc ggggtcggca gcggtacccg gatcccctcg acgcgcgcca
     2581 cttgcgccgg cgctgctccc gcagggatgc ccggagaggc attccataca ggccaagcgc
     2641 ccgggcccca gcctatcgcc gtcagtccgg ccagcaactg cacaacgggc atcatctcct
     2701 gtagctcctc tcctcgcggg gctgcgcacc gcccgcggag gggatgcctc cgcgggcgga
     2761 aagctaccta cttcgcctcg tcacgcgccc ggcgctcatc cacgacgatg gccacagc
//
LOCUS       DAOSCV010000601         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23139_length_2812_cov_3.212550, whole genome shotgun sequence.
ACCESSION   DAOSCV010000601 DAOSCV010000000
VERSION     DAOSCV010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23139_length_2812_cov_3.212550"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..961
                     /locus_tag="PLY56_13780"
     CDS             155..961
                     /locus_tag="PLY56_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ22598.1"
                     /translation="MDEHLTNPRRDRAGATLAHALVLATNRVAAPLQEQTAISLRARL
                     PVRGRPVLAWVLDALREASRIDSVVAVGPRSLAEEMGFPVGVRWVEEAETPAESVRRG
                     LRGLGLSTPVVVIPADLALLTPELIDDLIASFPLGVDVGCPLIRVKEFARAFPTLPQV
                     TMNFRDGRFVGGRVVVAKPQAVVMNYGLLQRAWRRPRWPEAAIGMLGMQTALLYSLRL
                     LSARQVAARATHRLMAPCAVLPDAPPELAFTLDTPEHFRLAQERQVAAPR"
     gene            complement(1191..1805)
                     /locus_tag="PLY56_13785"
     CDS             complement(1191..1805)
                     /locus_tag="PLY56_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22599.1"
                     /translation="MTARRFYTGVAACMLAAGIAASALSAPANEAPKETLEATATRVR
                     ELEKANVVLQEDLARLRLQLDLTREALDKARAGLEAETAARQALEAQLAQARQEATRE
                     IASSNEATRKELAGEMQTRLAALEQSIQALSARQQAELAALKEQHEARIASLQQALEK
                     ETAARQELEKETSQRIAGVELRQKRDRTWGFVMNGLNLGFTLSK"
     gene            complement(1874..2575)
                     /locus_tag="PLY56_13790"
     CDS             complement(1874..2575)
                     /locus_tag="PLY56_13790"
                     /inference="COORDINATES: protein motif:HMM:NF022100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22600.1"
                     /translation="MAGYLENAGGFDARDVTLRLALPAGLELVAGQPTMQFARLAPNE
                     TIQGSWTLRPNGKASGELPVTLTATSANIEQNSLTRRIQVHVPSPRLSTLVERVSVPL
                     ETNGLPTVVLVPVTLSPATSFVSARIVLTYDPEVIRAFDLSRGRAFVDQGALLPGWSY
                     DLSQPGRVIITATRGEAPPLTQAEANLGVVRFRSVAPGVSALKLEEVELINGKGERIP
                     VECLDAAITVIRAKE"
BASE COUNT          401 a          962 c          950 g          499 t
ORIGIN      
        1 ctctcgcgcg ggcgcggcta tctgcgccgt tccctggctg cctacgttca ggccggtgat
       61 cgtccctccc ggcgcctggc cgatgccgcg tgacgtggga gatggcctgc agcgccgtgc
      121 tgccacccct cgcccatagt aggagatagc ccccatggac gagcatttga cgaatccgag
      181 aagagatcgg gcaggcgcca cccttgctca cgcgctcgtc ctggctacga accgtgtcgc
      241 cgctcctctt caggagcaaa cggcgatctc gttgcgcgca cggcttccgg tgcggggccg
      301 gccggtgctg gcctgggtgc tcgatgcgct ccgcgaggcg tcacggatcg atagcgtggt
      361 tgctgttggc ccgcgcagcc tggcggagga gatgggattc cctgtaggtg tgcgctgggt
      421 ggaagaggcc gagaccccag cggagagcgt ccggcgcggg ctgcgcggat tgggtctgag
      481 cacgcccgtg gtggtgattc cggcggacct ggccctgctc actccggagc tgatagatga
      541 tctgatcgct tcttttcccc tgggggtcga tgtcggttgc ccgttgattc gcgtcaagga
      601 gttcgcgcgg gcgttcccaa cgcttcccca agtcacgatg aactttcgtg acggccgctt
      661 tgttggcggg cgcgtcgtgg tcgccaagcc gcaggcggtc gtgatgaact acggcctctt
      721 gcagcgcgcg tggaggcggc cccgctggcc cgaagcggcc atcggaatgc tcgggatgca
      781 gacggcgctg ctctactcgc tgcgcctgct ctccgcgcgg caggtagctg cacgcgctac
      841 ccaccggctg atggcccctt gcgcggtgct tccggatgcc cccccggaac tcgccttcac
      901 gctcgatacc ccagagcatt tccgtctggc gcaggagcgc caggtggctg cccctcgctg
      961 agcgccagcg gcgcgggtgc ccgcgccgcc caatgcaaac agcgccatct tcccgtgccc
     1021 gcgggcatcg ccgcccggca tccataccac ggatgagggg gacgggcgcg tgctttccta
     1081 ccccccggtt ccctccctac gctccggcgc tgcgccggcc ccagggaagg agagctaccg
     1141 tgagacggcg gcagacagtg ccgaaggggg ccggcaacgc cgtgggtggg ctacttcgaa
     1201 agcgtgaaac ccaggttcag gccgttcatc acgaagcccc aggtgcggtc gcgcttttgg
     1261 cgaagctcca ctcccgcgat gcgctgcgag gtctccttct ccaactcctg gcgcgccgcg
     1321 gtctcctttt cgagcgcctg ctgcagcgag gcgatgcgcg cctcgtgctg ctccttcaga
     1381 gccgccagct ctgcctgctg gcgtgcggag agggcctgaa tcgactgctc cagcgcggcg
     1441 agccgcgtct gcatctcgcc ggcgagctcc ttcctggtcg cttcgttgct actcgcgatc
     1501 tcgcgcgttg cctcctggcg cgcctgcgca agctgtgcct ccagggcctg gcgtgcggcg
     1561 gtctcggctt cgagcccggc acgcgccttg tccaacgctt cccgggtgag atccagctga
     1621 aggcgcagtc gcgccagatc ctcctgcagg acgacgtttg ccttctccag ctcgcgaacg
     1681 cgggtggccg tcgcctccag cgtctccttc ggtgcttcgt tcgccggcgc tgaaagcgcc
     1741 gaggcggcga taccggcggc cagcatgcac gcggccacgc ccgtgtagaa ccttctcgcg
     1801 gtcatctctt tccttccacg ccacataatc gctcgccggg cagaagcgtg tgcttccagc
     1861 ccaggtcgtt gtatcactct ttcgcccgga tgacggtgat ggcagcatcc aggcattcca
     1921 cggggatccg ctcccccttg ccgttgatca gttccacctc ttccagcttc agagcgctca
     1981 ctccgggggc cacgctccgg aagcggacca cgcccagatt ggcctcggcc tgggtgagcg
     2041 gcggcgcttc tccccgcgtg gcggtgatga tcactcgtcc gggctgggaa aggtcgtagc
     2101 tccacccggg aagcaacgcg ccctgatcca cgaatgcccg cccgcgtgag agatcgaacg
     2161 cccggataac ctccggatcg taggtcagaa cgatgcgcgc ggagacgaag ctcgtcgcgg
     2221 gggaaagcgt cacgggcacg aggacgacgg tggggagccc gttggtctcc aacggcaccg
     2281 agacgcgctc cacgagcgtg ctcagccggg gcgagggcac atggacctgg atccggcgcg
     2341 tgaggctgtt ctgctcgata ttcgcgctgg tggctgtgag ggtcacgggc aactcgccgc
     2401 ttgccttgcc gttgggacgc agcgtccaac ttccctggat tgtctcgttc ggggccaacc
     2461 tggcaaactg catcgtgggc tggccggcga ccagctccag gcccgcgggg agggcgagcc
     2521 gcaaagtcac gtcgcgtgca tcaaaaccgc ccgcgttctc cagatagccg gccacagtga
     2581 tcggctgggt acgctcgtgc aggaaagtgc tctcggaggg ggctgtgagc cccagcgcca
     2641 gcttgcccgg ctgcaggctg acaccgccga tgccgtaggc ggtgcgccat gatgcccaga
     2701 attatggcgg cacggctgga cacggcagaa aagcgcatta cggcggcggt ggagaagctg
     2761 gcggcggggc gggatgtacc ggccctgccg tttcaccgcg acccgcagca gc
//
LOCUS       DAOSCV010000602         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23198_length_2807_cov_3.224927, whole genome shotgun sequence.
ACCESSION   DAOSCV010000602 DAOSCV010000000
VERSION     DAOSCV010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23198_length_2807_cov_3.224927"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLY56_13795"
     CDS             complement(<1..1080)
                     /locus_tag="PLY56_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ22601.1"
                     /translation="MHFRRRLEAVTSGQWHACGPRVLRTVTLLSLCLPALLCSVPVIA
                     SEASDKAARVEAGTNRTADSSTSKKQAETEKDSLEQARPPLKRVEVGLVDRLATRESY
                     RTIAGKEYLLGPYDELSISVRGPKPETWSVTVSPQGVFEIPLVGVVRCAGMSVAELEK
                     SLTAKLNRYLRNFTLSIIVTRLRGIQVTVTGQVAVPGSYSLPGQASLWEAIQASGGIT
                     EAGSVRAVHVRSADGKERRVDLYPFLFQGQMQPGMTLAPGDTVHVPAVERIVGLDGEV
                     RRPGLYEFLPGETMAELLAFAGGPSAQAALDRAYVERILPDRQKLILAANLAPSSRQT
                     PPIELRDGDILTLPPVSLYLGSVEIT"
     gene            1717..2568
                     /gene="folD"
                     /locus_tag="PLY56_13800"
     CDS             1717..2568
                     /gene="folD"
                     /locus_tag="PLY56_13800"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOJ22602.1"
                     /translation="MAAEIIDGKAIAAEIRAEVARGVAALKESRGIVPGLAAVIVGEN
                     PASRTYVNMKRKACAEAGIHSEEYAFPEGISQQDLLSLVGRLNEDPRIHGILVQLPLP
                     EPLDAQEVLAAIDPEKDVDGFHPVNMGRLVLGLPGFVPSTPLGIMELLRRTGVSVAGK
                     HAVVVGRSHIVGKPAALLLLSQHATVTICHSRTADLPEVCRTAEILVVAVGKPEMVRG
                     DWIKPGATVIDVGINRVDGKLLGDVHFESASQVAGAITPVPGGVGPMTIAMLLRNTLD
                     AARRAVA"
BASE COUNT          485 a          877 c          932 g          513 t
ORIGIN      
        1 cgtgatctca acgcttccca gatagaggct gaccgggggc agtgtgagga tatcgccatc
       61 ccgcagctcg ataggcggcg tctggcggga gctcggggcg aggttggcgg cgaggatcag
      121 tttctgacgg tcggggagga tccgctccac gtaggctcgg tcgagtgccg cctgggccga
      181 gggccctccg gcgaacgcaa ggagttcggc catcgtctcc ccagggagaa actcgtaaag
      241 gccgggccgg cgcacctcac cgtcgagtcc cacgatgcgc tccacggcgg gcacatgcac
      301 cgtatcgcca ggtgcgaggg tcatgcctgg ctgcatctgc ccctggaaaa gaaacggata
      361 aagatcgacg cgccgctcct tgccatccgc gctgcggacg tggacggcgc ggaccgaacc
      421 cgcctccgtg atgccaccgc tcgcctgaat cgcctcccac agggatgcct ggcccggaag
      481 gctatagctg cccggcacgg ctacctgccc ggtcaccgtc acctggatgc cacgcaatcg
      541 ggtgacgatg atgctgagcg tgaaattgcg aagatagcgg ttcagcttgg ccgtgaggct
      601 cttctccagc tcggcgacac tcattcccgc gcatcggacg acgccgacca gggggatctc
      661 gaacacccct tggggtgaca cggtgacaga ccacgtctcc ggcttcgggc cacgcaccga
      721 gatggagagc tcgtcatatg gacccaggag atactccttg ccggcgatgg tgcggtaact
      781 ctccctggta gccaggcgat ccacgagccc gacctccacg cgcttcagag gcggcctggc
      841 ctgctcgaga gagtccttct cggtttcggc ctgcttcttc gaggtggagg agtccgcagt
      901 gcggttggtg ccggcctcca ctcgcgctgc cttgtccgac gcttccgagg cgatgaccgg
      961 cacggagcag agcagggcgg gcaagcagag tgagaggagt gtgaccgtgc gaaggactct
     1021 gggtccgcat gcgtgccact ggcctgaagt gaccgcctcg agacgtctcc ggaagtgcat
     1081 ggtcctgcag cctttcagtg ctcctggatt accgattccg cccccagagg gtgtgggatt
     1141 tatccccggc actcactcaa ctggtgcgag cttcgagacc ataggccacg tgtatccggc
     1201 ccgtgcctcc gaggcctggc ggcacacagt gagagcgcca acgtggttcg catgcccctg
     1261 gaagccgcaa cacctgatgg aagagagcgc tctgctatgc cggttatatc tggaagcgcg
     1321 cacaaccgcg gcagttccgg gaagtgttgc ggggatctgc gacctccagc aatacgccca
     1381 tcggctcgca cttgcacgcg aggagctgcc gctatccgct aactgtccac gacaaccgtt
     1441 cgtagcgtcg actctttcgc actgttgctc gctcgcgggt ccctttcagg tcctacgatg
     1501 gtgagtgcac tttggccatc aaccgtcaaa gcgagtgggc ttgcgctggc actcgatctg
     1561 tgacgaagaa ttcgcatcag gaggcctacg cgagctagtg taccagaaag gcaggagagt
     1621 gtcaagcctg cgttgacggc gcctggatgg tgtggtacaa taggcgccgt cacgaggccg
     1681 caagacagca ggactcgagg atggctggag gagagtatgg cggcagagat tatcgatggg
     1741 aaggcaatcg cggcggagat ccgcgcggaa gtggcccgag gcgtggcggc gctcaaagag
     1801 agccggggga tcgtgcctgg tctggcagcg gttattgtcg gtgagaaccc cgcgtcacgc
     1861 acctatgtca acatgaagcg caaggcgtgc gccgaagcgg gcatccactc cgaagagtat
     1921 gcattccccg agggtatctc tcagcaggat ctcctcagcc ttgtgggccg cctgaatgag
     1981 gatccccgca tccacggcat tctggtgcag ctcccgctgc ctgaaccgct ggacgcccag
     2041 gaagtgctcg ccgcgattga cccggagaag gatgtcgatg gctttcatcc cgtgaatatg
     2101 ggccgtctcg tcctcggcct gcccgggttc gttcccagca cgccgttggg cattatggag
     2161 ctgcttcgcc ggacgggcgt ttccgttgcg gggaagcatg cggtcgtggt gggccgcagc
     2221 catatcgtcg gcaagccggc ggcgctcctt ctcctctccc aacacgccac tgtgacgata
     2281 tgccactcgc gcaccgccga cctccccgag gtgtgtcgca ccgcggagat cctcgtggtt
     2341 gccgtgggca agcccgagat ggtgcgaggc gattggatca agccgggagc gaccgtgatc
     2401 gatgtaggca tcaaccgggt tgatggaaag ctcctgggtg acgttcattt cgagtcggct
     2461 tcgcaggtag caggggcgat cacccctgtt ccgggcggtg tcgggccgat gacgattgcc
     2521 atgctcctgc ggaacacgct ggacgcggca cgacgggcgg ttgcctgacg ccggggagaa
     2581 ggcctctcat tggagcgata tcggggtgaa cttcatctct cgccctctcg cgataggccg
     2641 caggcttcct cgagcgcgcc ctggctgagc gcacttgcat ggcttgtggc gctctgcggg
     2701 ctcttcctgc tcccctctca gcgcgcggcc ggtagccccc gactggctaa gttcgagccg
     2761 gcgcgcggat gctacctcgg cgcacacctg cgcccggtgg ggcctgg
//
LOCUS       DAOSCV010000603         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23252_length_2802_cov_3.401529, whole genome shotgun sequence.
ACCESSION   DAOSCV010000603 DAOSCV010000000
VERSION     DAOSCV010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23252_length_2802_cov_3.401529"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..657)
                     /locus_tag="PLY56_13805"
     CDS             complement(64..657)
                     /locus_tag="PLY56_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOJ22603.1"
                     /translation="MKARHWVWLWSVLAVAALPSGVLAASYEIDASHSTVGFRIRHLV
                     GRVNGSFTRFDGTIDFDEKAPAKGKVTATIDAASINTANEKRDEHLRNEDFFYVAKYP
                     KITFVSQKVTPNGKGRYRVLGTLTMRGVSRPTVLDVRLNGVAADPWGGTRAGFTATTK
                     LNRKDFGISWNQTLDRGGAVLGDEVEVTLEIEAVQKK"
     gene            856..1512
                     /locus_tag="PLY56_13810"
     CDS             856..1512
                     /locus_tag="PLY56_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ22604.1"
                     /translation="MAGRGVIFDLDGVLVDSSRLHWESWQRVGAERGFEMSEELFWET
                     FGMPNSLIFERLFGKPLPPEEAQELSERKEEIYRELAAGRLKPLPGAVELLGALKDAG
                     FRIALGSSTPMSNIRVILDAVGVRQFFDAIVCADDVTRGKPDPEVFLKAAERLGLPPA
                     RCVVVEDAVVGIQAAKAAGARCLAITTTHPREKLSEADRVVDSLEEVSPADFDALLAK
                     "
     gene            1734..>2802
                     /locus_tag="PLY56_13815"
     CDS             1734..>2802
                     /locus_tag="PLY56_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ22605.1"
                     /translation="MSSSRFSALPALLLAVFLLAIAGGCRRGGTPDGRITIRYMAWGN
                     PAQLQVERQIVSEFEKRHPNIRVHLFMVPGSSYLDKLQLMLASRTAPDVFRVDQYYFP
                     AIVRKGYFLPLDPLIAEEPPGFLDDFVPIALDEGRYQGKLYGMNVLFGAMMIYYNKDL
                     FRQAGLPDPYELDKQGKWSWDAFVDAARKLTVKDSSGRIRQFGTTVPSFPMYSSVVWN
                     EGGDFMNPDMTRMLIADDPRAVAAVQKLADLRWKYHCAPTPADSALSAFTFESGKIAM
                     VWGWAGESPRYRNSIRSFKWDIAPVPSGPAGSYSVVKGNQLCINALTKHSREAWEFVK
                     FMTGPEAELLLCGKLRRAVPTR"
BASE COUNT          461 a          952 c          846 g          543 t
ORIGIN      
        1 cgttatctct atgccgcggc cacctccttt ccttcaggcc acgcaggttc cccctcgcct
       61 ccctcacttc ttctgcacgg cctcaatctc cagcgtcacc tccacctcgt caccgaggac
      121 cgccccgccc cggtcgagcg tctggttcca cgagatacca aaatccttcc ggttcagctt
      181 cgtggtcgca gtgaaccctg cgcgcgttcc gccccagggg tcagcagcca ccccgttgag
      241 gcgcacatcg agaaccgtgg gccgggaaac cccgcgcatt gtcaacgtcc ccaacacgcg
      301 gtagcgtccc ttgccgttgg gcgtcacctt ctgactcacg aaagtgatct tcgggtactt
      361 tgcgacgtag aagaaatctt cgtttcgcag atgctcgtcg cgcttctcat tggcggtgtt
      421 gatgctcgcc gcgtcaatcg ttgccgtcac cttgcccttg gctggcgcct tctcatcgaa
      481 atcgatcgtg ccatcgaagc gagtgaagct cccattcacc cgccccacca ggtgacggat
      541 gcgaaagcca acggtggagt gcgaggcatc tatctcgtag ctagctgcca gtaccccgct
      601 cgggagcgca gccaccgcca agaccgacca aagccacacc caatgccttg ctttcatggt
      661 aacccatcct ctctgcccgt tctgtctcta gtaacacgca aacggccccg cgggttcccc
      721 ccgcgtccac gttctcacgg gcagcttcct agttatcgct ccacccggaa acatcatccc
      781 cgagcgcccc gtcctccggg ttcttgcctg cgaagcggcg gtatgctata ctgctggcgt
      841 tggagggaga agagtgtggc cggacgcggc gtgattttcg atctggatgg agtgctcgtc
      901 gattcctcgc gcctgcattg ggaaagctgg cagcgcgtgg gcgccgagcg cggctttgaa
      961 atgagtgaag agctcttctg ggagacgttc ggcatgccga actcgctgat cttcgagcgt
     1021 cttttcggga agccgctccc gcctgaggag gcccaggagc tttccgagcg caaggaggag
     1081 atctaccgcg agctcgccgc cggacggctc aagccgctcc cgggcgccgt ggagctcctc
     1141 ggggcgctga aggacgccgg cttccggatc gccctcgggt catccacgcc gatgtcgaac
     1201 atccgcgtga tcctcgacgc cgtgggcgtg cgccaattct tcgatgcgat cgtctgcgcc
     1261 gacgatgtca cccgcggcaa gcccgatccc gaggtcttcc tcaaggcagc cgagcgcctg
     1321 gggctccctc cggcgcggtg cgtcgttgtc gaggacgccg tcgtcgggat ccaggccgcc
     1381 aaagctgcgg gcgcgcgctg tctggccatc acgaccaccc acccgcgcga gaagctcagc
     1441 gaagccgacc gcgtggtgga ctcgctggaa gaggtctctc ccgctgattt cgacgccctc
     1501 ctggcaaagt agccggacca gggggcgctt ccgataccgg aggtcttccc tgcgcgcgtg
     1561 ggctcgccgc caggtttctt gccgtgctcg ttcgagccac gccggggcag cggaacccct
     1621 ctgcgccgtg gagggatggc tgccgggcgc cagaatagcg acagcggtta cggcaggcgg
     1681 cttggcctgt tgtgcttgac tatgctgccg gatctaatat gatggtaaac gagatgagta
     1741 gctcgcgctt ttccgcactt ccggcgctcc tcttagccgt ctttcttctg gccatcgcgg
     1801 gcggatgccg gcgcggcggc acgcccgacg gccggatcac catccgctat atggcctggg
     1861 gaaacccggc gcagcttcag gtggagcgcc agatcgtcag cgagttcgag aagcgccacc
     1921 ccaatattcg cgtccatctc ttcatggtcc ccgggtcgtc gtatctcgat aagctccagc
     1981 tgatgctcgc gtcgcgcact gcgcccgacg tcttccgcgt cgaccaatac tacttcccgg
     2041 cgatcgtgcg caaaggctac ttcctccccc tcgacccgct catcgccgag gagccgcccg
     2101 gcttcctcga tgacttcgtc ccgattgccc tggacgaggg ccgctaccag gggaagctct
     2161 acgggatgaa tgtcctcttc ggcgcgatga tgatctacta caacaaggat ctcttccgcc
     2221 aggccggcct gccggacccc tacgagctgg acaagcaagg gaaatggagc tgggatgcct
     2281 tcgtcgacgc ggcgcgcaag ttgaccgtga aggattcctc ggggcgcatc cgccagttcg
     2341 ggacgaccgt accctccttc cccatgtatt cctcagtggt gtggaacgag gggggcgact
     2401 tcatgaatcc cgatatgacg cgcatgctca tcgccgatga cccgcgcgcg gtggcggcgg
     2461 tgcagaagct cgccgacctg cgctggaaat atcattgcgc gcccacgccc gcggactcgg
     2521 cgctctcggc gttcaccttc gagtcgggca agatcgccat ggtatggggg tgggccggcg
     2581 agtcgccgcg ctaccggaat agcatccgca gcttcaaatg ggatatcgct cctgtgccct
     2641 caggccccgc agggagctac agcgtggtga aagggaacca gctctgcatc aacgcgctca
     2701 ccaagcactc tcgcgaggcg tgggagtttg tgaagttcat gaccggtccg gaagcggagc
     2761 tgctgctttg cggcaagctg cgccgtgccg tgcccacgcg cc
//
LOCUS       DAOSCV010000604         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23278_length_2800_cov_3.802186, whole genome shotgun sequence.
ACCESSION   DAOSCV010000604 DAOSCV010000000
VERSION     DAOSCV010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23278_length_2800_cov_3.802186"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1068)
                     /locus_tag="PLY56_13820"
     CDS             complement(166..1068)
                     /locus_tag="PLY56_13820"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HOJ22606.1"
                     /translation="MKRLLASRVLIVLGTFLMASGIAAAQQEEAILTSVPPEAIAFVS
                     ADIEQLRAFWEEIKGSTLGRLLAQELTRARPGAGAGADPRQIFEMLNLAFEDRVSLAL
                     MDEKWAPKQVPPVVVVIPLKNYEHARALLTVAQMQLQLQGRASFTSREYQGVQLTNVS
                     VSGLKDGQLGYALLEDRLLIATRPGWLEKAVDLAVQLGKGLAEDAAFQRVQALVAAKG
                     PAHAWFWWDPIRFNQRALEMAEEAARTAPAKGQPAAKPRSLPIQPAMLKQVMEAENLD
                     TKRWTPPEAAWSSAPKASWSTRSA"
     gene            1446..2507
                     /locus_tag="PLY56_13825"
     CDS             1446..2507
                     /locus_tag="PLY56_13825"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HOJ22607.1"
                     /translation="MASVDYRQRPHRRFNPLTREWVLVSPHRMQRPWQGAVEKEQEAR
                     RPTYDPQCYLCPGNERAEPPGGGPRPRNPQFTGTYVFRNDFSALLPDTPDTPVDTGHD
                     LLVVQPERGQCRVVVFSPRHDLTLAEMGDAEIRTVVDTWAEEYASLGALPYVNHVQIF
                     ENKGAIMGCSNPHPHGQIWAQERVPDGPAKELASMAEYRRARGGACLLCDYLAEESRR
                     GERLVCANRYFTALVPFWAVWPFETMILSHRHVTAITEFSDEERNALAEILRRLTTRY
                     DNVFEVSFPYSMGIHQAPTDGREHPECHFHMHFYPPLLRSAKVKKFMVGYEMLGEPQR
                     DLTAEASAERLRSMSEEHW"
     gene            2566..>2800
                     /locus_tag="PLY56_13830"
     CDS             2566..>2800
                     /locus_tag="PLY56_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22608.1"
                     /translation="MEVSVLEAISRMLHIAVEEQGSTLYVQVGDGTEVILRGEEGERR
                     ILKGPQRVFDQLRSRLLHMARLPAHDPSITREGE"
BASE COUNT          498 a          889 c          926 g          487 t
ORIGIN      
        1 cgagatcggg cgcaagctga tggcgcataa gggcgccaca ctccgctcgc cacgcctagc
       61 tactgccgac gtagaagatg gggtcggcag tagctaggcg tggcgagcgg agtggggcgc
      121 ccttatgcgc catcagcttg cgcccgatct cgttctcggc gttgatcacg ctgaccgagt
      181 cgaccacgat gcctttgggg ctgagctcca ggcggcctcc ggcggcgtcc agcgcttggt
      241 gtcgaggttc tcggcctcca tcacctgctt gagcatggcg ggctggatgg ggagtgaccg
      301 aggcttcgcc gcgggctggc ccttggcagg agcggtgcga gccgcttcct cggccatctc
      361 caacgcgcgc tggttgaacc gaatcggatc ccaccagaac cacgcgtgcg ctggtccctt
      421 ggcagcaacg agcgcctgca cgcgctggaa ggcggcatct tcggcaagcc ccttgccaag
      481 ctggacggcg agatcgaccg ctttctccag ccagcccggc cgggtggcga tgagcagccg
      541 gtcttcaagg agcgcgtagc ccagctgacc gtctttcagc ccactcacgg agacgttggt
      601 gagctggacg ccctgatact cgcgggaggt gaagctggcc ctcccctgga gctgcaactg
      661 catctgtgcc acggtgagga gcgcgcgagc gtgctcatag ttcttgagcg gtatcacgac
      721 caccacgggt ggcacttgct ttggcgccca cttctcgtcc atcagcgcca gcgagacgcg
      781 gtcctcgaac gccaggttga gcatctcgaa gatctgacgg ggatccgcgc cagcgccggc
      841 accgggcctg gctcgggtaa gctcctgggc caaaagccgg ccgagcgtcg agcccttgat
      901 ctcttcccag aaggcccgga gctgctcgat atcggcggag acaaaggcga ttgcctccgg
      961 tggcacggag gtgaggatcg cctcttcctg ttgggctgcg gcgattcccg atgccatcag
     1021 gaacgtgccg agaacaatga gcactcgcga ggcaagcaat ctcttcatcg tcatgtacct
     1081 cctcaatgcc tggagaggaa cactgataag ccgtcgtagg gcttgttcgt ctccccccat
     1141 cggagtccga tggccgccgc gagggatgga tgcagtcccg ggactggctg accatgaccc
     1201 tcggcatcgt ccccagcaga gaccgagaac ccatccgaag cgctcgctct ttcggaaagt
     1261 ctctccgcta tccgagagga ctctctgata ggctccaggt gctggctgta atgcgcccac
     1321 cgcattgtac cacgcgcgtg tctgggtgtc aacaagagga gaacgggggg tggctgccga
     1381 acagtccctt agtatttgac agttcgtgcc agcgcttcgc gccggcaggc accacggaga
     1441 cacggatggc atcagttgat taccggcagc gcccccaccg gcgcttcaat cccctcacgc
     1501 gggagtgggt tctcgtctcg ccgcaccgca tgcagcggcc ctggcagggc gccgtcgaga
     1561 aggagcagga agcacgacgg cctacctacg acccgcagtg ctatctgtgc cccggcaacg
     1621 agcgagccga gccgccgggg ggtgggccga ggccgcgcaa tccccagttc accggcacct
     1681 acgtttttcg gaacgacttc tcggcgctgt tgccggatac tcccgatacg ccggtggata
     1741 cggggcacga cctgctagtg gtgcagccgg aacgcgggca gtgccgcgtg gtggtcttct
     1801 cgccgcggca cgacctgacg ctcgcggaga tgggcgatgc cgagatccgc actgtggtgg
     1861 atacttgggc ggaagagtat gcctcgctcg gcgctctccc ttacgtgaat catgtccaga
     1921 tcttcgagaa caaaggggcg atcatggggt gcagcaaccc ccatccccac gggcagatct
     1981 gggcgcagga gagggtgccg gatggcccgg cgaaggaact ggcctcgatg gcggagtacc
     2041 ggcgcgcgcg cggaggcgcc tgcctcctat gcgactacct tgccgaggag tcgcggcgcg
     2101 gtgagcgcct ggtgtgtgcc aaccggtact tcacggcgtt ggtgccattc tgggcggtgt
     2161 ggcccttcga gacgatgatt ctcagtcacc gccacgtgac ggcgatcacc gagttctcgg
     2221 atgaggagcg caatgccctc gctgagatct tacgccggct gacgacgcgc tacgacaatg
     2281 tcttcgaggt ctcgttcccc tactcgatgg ggatccacca ggcgcccacg gacgggcgcg
     2341 agcatccaga gtgccatttc cacatgcact tctacccgcc gctcctccgt tcggcgaagg
     2401 tgaagaagtt catggtgggc tacgagatgc tgggagagcc ccaacgcgac ctcacggccg
     2461 aagcaagcgc ggagcggctg cgctccatga gcgaagaaca ctggtgagcc gaacactcag
     2521 ccggcgggcc aggtgtctat agggtgtgga aggatgcggt ggacgatgga ggtctcggta
     2581 ctcgaggcga tcagcagaat gctgcacatc gcagtggagg agcaggggag cacactctat
     2641 gtgcaggtgg gggatggcac cgaggtcatc ctccgcggtg aagagggcga gcgacgcatt
     2701 ctgaaaggcc cccagcgcgt tttcgaccaa ctgcgctcgc gcctgctcca catggcccgc
     2761 ctccccgcgc acgacccttc catcacccgt gaaggcgaga
//
LOCUS       DAOSCV010000605         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23279_length_2800_cov_3.332969, whole genome shotgun sequence.
ACCESSION   DAOSCV010000605 DAOSCV010000000
VERSION     DAOSCV010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23279_length_2800_cov_3.332969"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="PLY56_13835"
     CDS             <1..2036
                     /locus_tag="PLY56_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22609.1"
                     /translation="DRMIEYCRRSGIRQVLISSGSWCASPGHYLFNQQRFPDGEAGLK
                     RLVDKLHAAGIKVGMHAFVSKVAKRDPYVTPKPDRRFWVDRKSTLAGAITADQTEIRC
                     ATDLRDWPGSPVAQQKTWEGGVKKHQEVILGDEIIQYDSIGPEGRWDTFLGCKRGAWG
                     TKPAAHPAGTEALHYGVDGCINGYIIDQETDLMDEVAERLAGIFNRCGFDMIYFDGGE
                     DVDRRRYFYYASNFQEQAMRRFQRRPVIHMGTVMTHLLWHSFSRSATVDTYINTLRGA
                     IVAGASVENWPTVRGHIDKSVRYMLSMRQDLMPGELGWFGIWPKDRDTDGLQFDEFEY
                     LLCKSLAYDAPVSLETSFEQMEAHPLTPGLLDLFRVYDDLRMRRAVDEATRKQLAEQG
                     KDFALLLAPGKAPRFIPVEPLEKVGGSRDVRACVGEIENQGVATIWHAFRDGQLLLDL
                     QPREVRATNLEGRQIQVTTEAGKAIVPVGPVRTTLYFPGRSAAQVSALLERAAVRTRP
                     PTRIFIEAEAYSRIEGAMAKGSTVGIREPEASGDVIVPTKAADRAQPQPWYCEYTVQI
                     PHDGRWTLWARVRYPSGADDSFAVVLPDEEPTLAMSKVLGNCGQHDKKWHWTGRGGGS
                     TTPPPGVPVAFNLKKGPFTFRVYAREGAGQLALAPRIDRLCLSDIDTPPGEEE"
     gene            complement(2163..2522)
                     /locus_tag="PLY56_13840"
     CDS             complement(2163..2522)
                     /locus_tag="PLY56_13840"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOJ22610.1"
                     /translation="MRLITVIKSKIHRATVTDADLDYVGSITIDRDLIERAGLVPGEK
                     VHVWNVTNGQRFETYVMPGERGSGIICVNGAAAHRVRVGDKVIIAAFVLTDEPVEPRM
                     ILVDEQNRFAGTLHLTP"
     gene            2668..>2800
                     /locus_tag="PLY56_13845"
     CDS             2668..>2800
                     /locus_tag="PLY56_13845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013190273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ22611.1"
                     /translation="MGNTFGHAFRITTWGESHGGAVGVAIDGCPPGMELSLEEIQREL
                     "
BASE COUNT          496 a          924 c          907 g          473 t
ORIGIN      
        1 ccgaccgcat gatcgaatac tgccggcgct ccggcatccg ccaggtgcta atctccagtg
       61 gctcgtggtg cgcctcgcct gggcactatc tcttcaacca acagcgcttt cccgatggcg
      121 aggcaggtct gaagcgcctt gtggataagc tgcacgccgc cggcatcaag gtgggcatgc
      181 atgccttcgt ctccaaggtc gcgaaacgcg atccctacgt cacgccaaag cccgaccggc
      241 gcttctgggt cgaccggaag agcaccctcg caggagcgat caccgccgac cagacggaga
      301 tccgctgcgc aaccgatctg cgcgactggc ccggcagccc ggtcgcacag caaaagacgt
      361 gggaaggcgg agtgaagaag caccaggagg tgatcctggg agacgagatc atacaatacg
      421 attcgattgg tcccgagggc cgttgggaca ccttcctggg ctgcaagcgt ggcgcctggg
      481 gcacgaagcc cgcggcgcac cccgccggca cggaggcgct ccattatggc gtcgatggct
      541 gtatcaacgg ctacatcatc gatcaagaga cggacctgat ggatgaggtc gccgagcgcc
      601 tggcaggtat cttcaaccgt tgcggcttcg atatgatcta cttcgacggc ggcgaggatg
      661 tggaccgcag gcggtacttc tactatgcct cgaacttcca ggagcaggcg atgcgtcgct
      721 tccagcgccg tcccgtcatt cacatgggca cggtgatgac gcacctcctc tggcactcgt
      781 tctctcgaag cgccacagta gatacctaca tcaacaccct gcgcggagcc atcgtcgccg
      841 gcgcgtccgt ggagaactgg cccaccgtgc gcgggcacat cgataagtcg gtgcgctaca
      901 tgctctcgat gcgccaggat ctgatgccgg gcgagctagg ctggttcggc atttggccca
      961 aagaccgcga taccgacggc ctccagttcg acgagttcga gtatctgctg tgcaagtcac
     1021 tcgcttatga cgcgcccgtc tccctggaga cgagcttcga gcagatggag gcgcacccgc
     1081 tcacccccgg attgctggat cttttccggg tgtatgatga cctgcgcatg cgccgcgccg
     1141 tggacgaggc gacgcggaag cagctcgccg agcagggcaa agatttcgcc ctcttgctcg
     1201 ctcccgggaa ggccccgcga ttcattccgg tggagcctct ggagaaggtg ggcggcagcc
     1261 gggatgtgcg cgcctgcgtc ggtgagatcg agaaccaggg cgtggccacg atctggcacg
     1321 ctttccgtga tggccagctg ctcctcgatc tccagccgcg cgaggttcgc gccacgaatc
     1381 tggaaggccg tcagatccag gtcacgaccg aggccggcaa ggccatcgtc ccagtagggc
     1441 cggtgcgcac cacgctttat ttccccgggc gaagcgcggc acaggtgagc gccctcctgg
     1501 agcgtgccgc ggtgcggacc cgccccccga ctcgcatctt cattgaagcc gaggcctatt
     1561 ctaggatcga gggcgcgatg gcgaagggat cgaccgtggg aatccgggag ccagaagcca
     1621 gtggcgatgt gatcgtgccg acgaaggcgg ccgaccgcgc ccaaccccag ccctggtact
     1681 gcgagtatac cgtgcagatc ccgcacgacg gacgatggac cctctgggca cgcgtccgct
     1741 acccgagcgg agcggacgac tcctttgccg tcgttctgcc cgacgaggag cccacgctcg
     1801 cgatgtcgaa ggtgctaggg aactgcggcc agcacgacaa gaaatggcac tggaccgggc
     1861 gtgggggggg gtcgaccacg cctccccccg gcgtaccggt cgccttcaac ctgaagaagg
     1921 ggcctttcac cttccgcgtc tacgcgcgcg agggcgctgg ccagctcgcg ctcgcgcccc
     1981 gcatcgaccg cctctgcctc agcgatatcg atactccgcc cggcgaggag gagtgacagg
     2041 acatcgctcg ctaccgaagg cacgttgtga cgcccgcgcg gttctgcttc gcggagccgc
     2101 gcggcgcgtg cccggcttca gagcggagtg tcggccggcc gttgagagga gagcggtagg
     2161 ggtcacgggg tgagatgtag cgtgccggcg aagcggtttt gctcatcgac caggatcatc
     2221 cggggctcca ccggctcatc ggtgagcacg aacgccgcga tgatcacctt atcgcccacg
     2281 cgcacgcggt gcgccgccgc gccgttgacg cagatgatcc cgctgccgcg ctcgccgggc
     2341 atcacgtaag tttcgaaacg ctggccgttg gtcacgttcc agacgtggac cttctcgcca
     2401 ggcaccagac cggcccgctc gatcaggtca cggtcgatgg tgatgctgcc cacgtaatcg
     2461 aggtcggcat cggtcacagt cgcccggtga atcttactct tgatcactgt gatgagacgc
     2521 acgctccata ctccctgggc cgcggacgca caccggcggc ccagtgggaa ttatagcact
     2581 gcctgcccgc tccgtcaaac gcggcacgcg gcgcgcccat tccaggcggg cgttgacgcc
     2641 catccccggc tgtgataaga tacgcccatg ggaaacacct tcggccatgc attccgtatc
     2701 acgacgtggg gcgagtcgca cggcggcgct gtcggcgtcg ccattgacgg ttgcccccct
     2761 gggatggagc tctccctcga ggagatccag cgcgagctcg
//
LOCUS       DAOSCV010000606         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23281_length_2800_cov_3.178506, whole genome shotgun sequence.
ACCESSION   DAOSCV010000606 DAOSCV010000000
VERSION     DAOSCV010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23281_length_2800_cov_3.178506"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLY56_13850"
     CDS             <1..352
                     /locus_tag="PLY56_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ22612.1"
                     /translation="PDVIRKKIKRAVTDSGSEILYHEEKPALSNLLTIYHLMSGIPIS
                     ELEERYRGVGYAQFKRDLADVIIEHLRPFQERYEGLDDAEVIRILREGASRARVVAEQ
                     TVRQVKERMGIVVE"
     gene            359..1054
                     /locus_tag="PLY56_13855"
     CDS             359..1054
                     /locus_tag="PLY56_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22613.1"
                     /translation="MASRILIVEDEKGIADGLAFTLRQEGYQTTIARTGKEALDSFAK
                     REPDLVLLDLMLPDTDGLAVCRTMRRTSTVPILMLTARDSEMDKVVGLEVGADDYITK
                     PFGIQELLARVKAALRRARVRDQERGEEPQGQLVAGEIVLDPVTRTVEVHGRPVELRP
                     KEFDLLRVLITHPGRVHRRQDLYAEIWSEADYVDRGTLDVHIRWLREKIEEDPGHPRH
                     ILTIRGVGYKIVP"
     gene            1108..1794
                     /locus_tag="PLY56_13860"
     CDS             1108..1794
                     /locus_tag="PLY56_13860"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOJ22614.1"
                     /translation="MRIVGEEFDDLVARRIQEWQLTQRRRGEMPATPQEAMRRVITLS
                     RVLGSGGSETAQHLHEATGWEIWDRRIVEEIARSAQVRMRMVESVDERAHSEIIAMVD
                     QLLGERMEQAGYRRHLAEVVLTIARLGNAIIIGRGTNWLLPDALNVRIVAPMKQRVER
                     IARREGISKSEAERLCRRSDRERAEFVRALFDKAIDDVTGYDLVINTQHLEPQAAAAV
                     ILAAVPQRFG"
     gene            complement(1859..>2800)
                     /locus_tag="PLY56_13865"
     CDS             complement(1859..>2800)
                     /locus_tag="PLY56_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22615.1"
                     /translation="MPLCHLITWLDPKFLEVYIIGAWHMAYNFMDRRYIPAGVEFLDK
                     GIHNNPDEAELRFEQANMLHDKAQDFERAAERAREANDMNLQPAGKRHLLAHALEKAG
                     RIDEAIMAWEEFIQEEEAAGNWQMANVSRHNRDLTIWRREARKLRAEDPLHVEFDIKW
                     SVPKRRVVRIEGTTNLPELTKVNVQLRDKGYEQILRDHPQVLWQVANTSLYWDNFTVK
                     DGKWSTWYKSGNLIVDRLDLGSEPAKYPLRSDEYEVVITVNPRTEPIIVQDITGWDGE
                     GITGPHVQEIDGVRMIRKVFTITRDELLGVTQVTQAN"
BASE COUNT          503 a          838 c          933 g          526 t
ORIGIN      
        1 cccggatgtg atccggaaga agatcaagcg cgcggtgacc gactccggga gcgaaattct
       61 ctaccatgag gagaagccgg cgctctcgaa tctcctcacc atctaccacc tgatgtccgg
      121 catccccatt tcggagctag aggagcggta tcgtggggtg ggctatgcgc agttcaagcg
      181 cgatctcgcg gacgtcatca tcgagcacct ccggccgttc caggagcgct acgagggcct
      241 ggatgacgcc gaggtgatcc gcattctgcg tgagggcgcc tctcgcgccc gggttgtggc
      301 cgaacagacg gtacgccagg tgaaggagcg gatgggcatc gtggttgaat aggtcatcat
      361 ggcaagccgc atactgattg tcgaggatga gaagggcatt gccgatggtc ttgccttcac
      421 ccttcggcaa gaaggctacc agacgacgat cgctcggact ggcaaagaag cgctcgactc
      481 cttcgcgaaa cgcgagccag acctggtgtt gctggatctg atgctgccag acacggacgg
      541 gctggccgtc tgccgcacca tgcgacggac gagcacggtt cccatcttga tgctcaccgc
      601 ccgtgacagt gaaatggaca aagtcgtcgg cctcgaggtg ggtgccgacg attacatcac
      661 caagccgttc gggattcagg agttgctggc ccgggtgaaa gcggctctcc ggcgcgctcg
      721 tgttcgtgac caggagagag gtgaggagcc ccagggacaa ctggtcgcag gagagatcgt
      781 actagacccc gtcacccgca cggtcgaggt gcatggccgg cctgtcgaac tgcgcccgaa
      841 agagtttgac ctccttcggg tgctgatcac ccatccggga cgggttcacc ggcgccagga
      901 tctctacgcg gagatctgga gcgaggcgga ctacgtcgat cgcggtactc tcgatgtcca
      961 tatccgttgg ctgcgcgaga agattgagga ggacccgggc cacccgcggc acatcctcac
     1021 cattcgtggc gtcggctaca agatcgtgcc gtgaaatccc ctgaaatcgt ggctcctgag
     1081 caggtccacc gaggaaggga gggtgcgatg cggatcgtgg gagaggagtt cgacgacctg
     1141 gtagcgcgcc gtattcagga gtggcaactc actcagcggc gccgcggcga gatgcctgcc
     1201 accccccaag aggcgatgcg gcgcgtgatc accctttcac gcgtgctggg gagcggcggc
     1261 agtgagactg cgcaacatct ccacgaggca actggctggg agatctggga ccggcggatc
     1321 gtggaggaga tcgcccgttc agcccaggtg cgcatgcgca tggtggagag cgtggacgag
     1381 cgcgcccact cggaaatcat cgccatggtg gaccagctgc tcggcgagcg catggagcag
     1441 gcgggctatc ggcgccacct ggcggaagtg gttctgacca tcgcccggct cggcaacgcg
     1501 attatcatcg gccggggcac gaactggctg ctccccgacg cgctgaatgt gcgcatcgtg
     1561 gcgccgatga agcagcgcgt ggagaggatc gcccggcgcg agggaatctc gaagagcgag
     1621 gcggagcggc tctgccgccg atctgaccga gagcgcgcgg agttcgtccg cgcgttgttc
     1681 gacaaggcga ttgatgatgt gaccgggtat gacctggtga tcaacacgca gcacctggag
     1741 cctcaggcag ccgcggcggt gattctcgcg gccgtgcccc agcgtttcgg gtaacccggt
     1801 gtgactaaaa tgggcgtggc cggcccctgg ggccggccac gcccattttg catggcagct
     1861 agttggcctg ggtcacctgc gttaccccga ggagctcatc acgggtgatg gtgaacacct
     1921 tgcggatcat ccgcacgccg tcgatctcct gcacatgcgg gccggtgatc ccctcgccgt
     1981 cccatcccgt gatatcttgc acgatgatcg gctcggtacg cgggttgacc gtgatcacca
     2041 cctcgtactc atctgacctg aggggatact tggcgggctc ggagcccagg tcgaggcgat
     2101 ccacgatgag gttgccgctc ttgtaccagg tggaccactt gccatctttg acggtgaagt
     2161 tgtcccagta gagcgaggtg ttggccacct gccacagcac ctgcgggtgg tcacgaagga
     2221 tctgctcgta gcccttatcg cggagctgga cattcacctt tgtcagctct gggaggttcg
     2281 tagtgccctc gatgcggacg acgcgccgct tggggacgct ccacttgatg tcgaactcca
     2341 cgtgcagcgg atcctcggcg cgcagtttgc gggcctcacg ccgccagatc gtcaggtcgc
     2401 ggttatggcg ggagacgttg gccatctgcc agttcccggc ggcctcctcc tcctggatga
     2461 actcctccca ggccatgatc gcctcatcga tgcggccggc cttctccagg gcgtgggcaa
     2521 gcaagtgtcg cttgcccgca ggttggaggt tcatatcgtt ggcctcgcgc gcgcgttcgg
     2581 ctgcccgttc gaagtcctgc gccttgtcgt ggagcatatt cgcctgctcg aagcgcagct
     2641 ctgcctcgtc cggattgtta tggatgccct tatcgaggaa ctcgacgccc gcggggatgt
     2701 agcgcctgtc catgaagttg taggccatgt gccaggcacc gatgatgtac acctccagaa
     2761 acttgggatc gagccatgtg atcaggtggc agaggggcat
//
LOCUS       DAOSCV010000607         2799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23291_length_2799_cov_3.195335, whole genome shotgun sequence.
ACCESSION   DAOSCV010000607 DAOSCV010000000
VERSION     DAOSCV010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23291_length_2799_cov_3.195335"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2186
                     /locus_tag="PLY56_13870"
     CDS             <1..2186
                     /locus_tag="PLY56_13870"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOJ22616.1"
                     /translation="ALNEGLLRLESEPGETALIDELFRHAHSIKGSAQMVGLDLVSRV
                     AHGLEDALGAVRSGKLSVSPPLLDVSFQTLDLLERLLEDPPDQAALSQAEGLAEHLAA
                     IGAGGPVSRYDAAALMTADAGGEEGVPDGPATPALPAEGETEWPEAREQAAPERPAAT
                     AAPAAAGRMADVMRVPIERVDHLLQLVGELASLEQQGRALAEALNAHQAQLRRFRGSA
                     GSPEAGLTGEQLEKLEQACARASRLVRTARRLVTAQAILGREIRDAAMDLRLLPVATL
                     FERFPRPVRDLARAAGKEVRLVIEGEETRLDKRILDELYDPILHLLRNAVDHGIEPPA
                     ERVEAGKPEQGTILLRAMPRGRTVCIEVSDDGRGISIEKVRQAACDRALITPGEAAGL
                     TEDQVLQLLFRPGFSTATQVTSVSGRGVGLDVVYSNVHDLKGVVRLENRPGEGATFSL
                     EIPLTLATTRVLLLECGGVAYALPGALVQGVMEVDAAAARAACGQQVISWKGRTVPLW
                     PLDAVLGRSQRPPAGNRLAVLVTNAGSTVGLMVDRLLDEEEVVLKPVGALLESARLVT
                     AGAVLGDGRIALLLDVDAVVKAASHVRAGATGTTESESARRYSILLADDALTTREVER
                     AILEAAGYEVTAVADGQAAWEQLARRSFDLVVTDVEMPRMDGIALTQQITSDPRTARI
                     PVIIISSLAKEEDRRRGLEAGAAAYIVKSAFDQENLLDLIERLIR"
     gene            2252..2797
                     /locus_tag="PLY56_13875"
     CDS             2252..2797
                     /locus_tag="PLY56_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22617.1"
                     /translation="MLLLAGLGFGGLVSAWVAQAQARASDPLFGALFAVMVVLLLAAA
                     SIGVRAILSIRRDLRTAAVDLSGTMAHLSAAVPALSATASQQASGAAEQAAAVTETTV
                     TVEELSRTAKQIAHAAGEAMAAAEEGHAAVQETGEGMERIKQRMDDVASRILSLGEKS
                     QRVGEIMRSSPISPGRHTCSR"
BASE COUNT          434 a          859 c         1081 g          425 t
ORIGIN      
        1 aggcgctgaa cgagggtctg ctccgcctgg agagcgagcc gggcgagacc gcgctcatcg
       61 acgagctgtt tcggcatgcc cacagcatca agggctcggc gcagatggtc ggcctcgatc
      121 tggtcagccg cgtggcgcac ggcctggagg atgccctggg cgccgtgcgc tcgggcaagc
      181 tatccgtctc tccccctctc ctcgacgtga gcttccagac tctggacctt ctggagcgcc
      241 tcctggagga tccgccggac caggccgcgc tgtcgcaggc agaggggctt gccgagcacc
      301 tggcggccat cggcgcgggg ggcccggtct cccgctacga cgcggccgct ttgatgaccg
      361 cagatgcagg gggagaggag ggggtgccgg atggcccggc gactccggca ctgcccgcag
      421 agggcgagac ggagtggcca gaggccaggg agcaggcggc cccggagagg ccggccgcga
      481 ccgcggcgcc ggcggccgcc gggcgcatgg ccgacgtgat gcgcgtgccg atcgagcgcg
      541 tggatcacct gcttcagctc gtcggcgagc tcgccagcct ggagcagcag gggagagccc
      601 tcgcggaagc gctgaacgcc catcaggcac agctgcggcg gtttcgcgga tccgctggca
      661 gtccggaagc ggggctcacg ggcgagcagt tggagaagct tgagcaggcg tgcgcgcggg
      721 cgagccggct ggttcgaacc gcgcgccggc tggtgaccgc ccaggcgatc ctcgggcgcg
      781 agattcgaga cgcggcgatg gacctccgcc tcttgccggt cgccacgctc tttgaacgtt
      841 tcccgcgccc ggtgcgcgac ctggcgcggg cggcaggcaa ggaagtccgt ctggtgatcg
      901 agggcgagga gacgcgcctg gacaagcgga tcctggatga gctgtacgat ccgattctgc
      961 atctcctccg gaacgcggtg gatcatggca tcgagcctcc cgcggagcgg gtggaagcgg
     1021 ggaagcccga gcagggcacc atcctcctgc gcgccatgcc gcgcgggcgc acggtctgca
     1081 tcgaggtgag cgacgatggc cgaggcatct ccatcgaaaa ggtgcgccag gccgcgtgcg
     1141 accgggcgct catcacgccg ggcgaggcgg cggggcttac cgaggatcag gtgttgcagc
     1201 tgctcttccg cccgggcttc agcacggcga cgcaggtgac ctccgtttcc ggccggggtg
     1261 ttggcctcga tgtggtctac agcaacgtac atgatttgaa gggggtggtg cgcctggaga
     1321 atcgcccggg agagggcgcc accttctcgc tggagatccc gctgacgctg gcaacgacgc
     1381 gcgtgctcct gctggagtgt ggcggcgtgg cctatgcgct tccgggggcg ctggtgcagg
     1441 gcgtgatgga agtggacgcc gccgcggcgc gggcggcatg tggccaacag gtgatctcct
     1501 ggaaaggacg cacggtgcct ctctggcccc tggatgccgt tctgggccgc tcgcaacgcc
     1561 ctcctgctgg aaaccgcctg gccgtcctgg tgacgaatgc cgggagtacc gttggcctga
     1621 tggtagaccg cctcctggat gaggaggagg tggtgttgaa gccggtcggg gcgctgttgg
     1681 agagtgcgcg cctagtgacg gccggcgcgg tgctggggga tggccgaatc gcgctgctcc
     1741 tcgacgtgga tgccgtggtg aaggcggcct cgcacgtacg ggcaggggcg accggcacca
     1801 cagaaagcga gagcgcccgg cgatactcca tcctgctcgc ggatgacgcg ctgacgacgc
     1861 gggaagtgga gcgcgcgatc ctggaggcag ccgggtatga ggtgaccgcc gtggcagatg
     1921 gtcaggcggc gtgggagcag ctggcgcggc gctctttcga tctggtggtg acggatgtgg
     1981 agatgccccg catggatggg atcgcgctca cccagcaaat cacctcggat ccacgcaccg
     2041 cgcgcatccc tgtgattatc atctcgtcgc ttgcgaagga agaggaccgg cgccggggcc
     2101 tggaggcggg cgctgccgcg tatatcgtga agagtgcctt tgatcaggag aacttgcttg
     2161 acctgatcga gcggctgatc cgctgaaccg gaaagagagt gtgtgagcaa tgagcatcgc
     2221 gaaacgtgtg ctcagagatg tgggagtgct catgttgttg ctggctgggc tgggattcgg
     2281 cggcctggtc agcgcttggg tcgcgcaggc gcaggcgcgc gcgagcgacc cgctcttcgg
     2341 cgcgctcttc gcggtgatgg tggtgctcct ccttgccgcc gcgagcatcg gcgtccgggc
     2401 gatcctgagc atccgccggg acctccgcac ggcggcggtg gacctgagcg gcacgatggc
     2461 tcatctctcc gccgcggtgc cggcgctcag cgccacggcg tcgcagcagg cgtcgggcgc
     2521 ggcggagcag gcggctgcgg tcaccgagac gacggtgacg gtcgaggagc tctcgcgaac
     2581 agcgaagcag atcgcgcacg cggcgggcga ggcgatggcc gcggccgaag agggccacgc
     2641 cgcggtgcaa gagacgggcg aggggatgga gcggatcaag cagcggatgg atgacgtggc
     2701 gagccgcatc ctctcgctgg gcgagaagag ccagcgcgtg ggcgagataa tgagatcatc
     2761 accgatatcg ccgggaagac acacctgctc gcgctgaac
//
LOCUS       DAOSCV010000608         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23364_length_2793_cov_2.894449, whole genome shotgun sequence.
ACCESSION   DAOSCV010000608 DAOSCV010000000
VERSION     DAOSCV010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23364_length_2793_cov_2.894449"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLY56_13880"
     CDS             <1..550
                     /locus_tag="PLY56_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="guanylate cyclase"
                     /protein_id="HOJ22618.1"
                     /translation="AAIAAIRSHQAELQAQEKSLAFMALDVVGSTKMKVSEKPLIIEH
                     AFLAFREWVESIMRQHGVWKSSWTPDGVMAAFPDVNEAVAAAQEVLEGLPKFNKEGHE
                     MSVPFQVRLGVSQGNVIFDDDARMDEVSHQVIDLAGHLQKYASPDTLLMAKHDYAKLR
                     DRKGFRTASQNVDGYETLEWKA"
     gene            574..993
                     /locus_tag="PLY56_13885"
     CDS             574..993
                     /locus_tag="PLY56_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22619.1"
                     /translation="MPKRSIREQEEHDLAIRRIARARFASTPEWEAFTNPGESKHYAL
                     ILPDGRRLYPDLVARRKGAHASSYVIEVETDSTVTEEEAQQWKALSDLGRRFLLFIPA
                     GHLLRARELCHRLGITVHGYRVYELTPFWIRIRNFRV"
     gene            complement(1027..1788)
                     /locus_tag="PLY56_13890"
     CDS             complement(1027..1788)
                     /locus_tag="PLY56_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOJ22620.1"
                     /translation="MDEQIKIQVDQFSAAYGNQEVLHNITLPVRRNEILAVFGPAGGG
                     KTTLLRSMNRLVDNTRHARHTGRILLDGRDIFAPEMDVVGLRRRVGMIYALPVPLPMS
                     VYDNIAYGPRMSGIRDRKRLDEIVERSLVRAAMWDEVCDRLDESALHLSGGQQQRLSI
                     ARVLAMEPEVILLDEPTAALDPVSTHKIEQTLFDLKRQCTVVIVPHNTQQAARLADHA
                     AFILMGELVEYNTGHQIFTSPQDRRTEDYITGRFG"
     gene            complement(1873..2739)
                     /gene="pstA"
                     /locus_tag="PLY56_13895"
     CDS             complement(1873..2739)
                     /gene="pstA"
                     /locus_tag="PLY56_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOJ22621.1"
                     /translation="MISEKRHSTWPHVTQRVAFGLIWASALGTLVVLGFILAYILRLG
                     LPHVTWSFLTTAPEAMGREGGILPTIVGTIALTLGAVVIAAPLGIGAAIFLTEYTWES
                     PITRAIRFGAEALAGVPSIIFGLFGFIFFVSYLKMGWSIASGALTLAIMILPTIIRTA
                     EEAILTVPHPYRDVSFGLGATRWQMVTTVVLPTALPGIITGIILAIGRSVGETAAVIF
                     TAGTALNMPTSLLSPARTMSVHFYILAIEGISLEKAYATGAILIITTLVINVLANWLI
                     VRAVRRNRRRPT"
BASE COUNT          531 a          864 c          925 g          473 t
ORIGIN      
        1 ggctgctatc gccgcgatcc gcagccacca ggcggagctg caagcccagg agaagagcct
       61 cgctttcatg gccctcgatg tcgttggctc gacgaagatg aaggtctccg agaaaccgct
      121 gatcatcgag cacgccttcc tcgcgtttcg cgagtgggtg gagagcatca tgaggcagca
      181 cggcgtctgg aagagctcct ggacgccaga tggcgtgatg gcggcgtttc cggacgtgaa
      241 tgaggcggtg gccgccgctc aagaggtgct cgagggcctg cccaagttca ataaggaagg
      301 gcacgagatg agcgtgccct tccaggtgcg cctgggcgtc agccagggga acgtgatctt
      361 tgatgacgac gcgcgcatgg atgaggtgag ccatcaggtg atcgacttgg ccggccacct
      421 gcagaagtac gcgtcgcccg ataccctcct gatggcgaaa cacgactacg ccaagctgcg
      481 cgaccggaaa ggcttccgaa cggcgagtca gaacgtggac ggttatgaga ccctcgagtg
      541 gaaggcctga ccgcgggaga gaccgtatcg cccatgccaa aacgcagtat ccgcgagcaa
      601 gaggagcacg atctcgctat ccggcggatc gctcgcgccc gcttcgccag cacgcccgag
      661 tgggaggcct tcaccaatcc gggtgagagc aagcactatg cccttatcct gccggacgga
      721 cgccgcctct accccgacct cgtcgcccgc cggaagggag cgcacgcctc cagctatgtg
      781 atcgaggtgg aaaccgacag cacggtcacc gaggaggaag cgcagcagtg gaaggcgctt
      841 tctgatttgg ggaggcgctt tctgctcttc atccctgccg ggcaccttct gcgcgctcgc
      901 gagttgtgcc accgcttagg gatcacggta cacggctacc gggtctacga gctgacgccc
      961 ttctggatcc gcatccgcaa ctttcgggtg tgacgcacgc cctcacggcc cagggagccg
     1021 tcccgtttag ccgaagcgcc cggtgatgta gtcctccgtg cgccgatcct ggggcgaggt
     1081 gaagatctgg tggccggtgt tgtactccac caactctccc atgaggatga acgcggcgtg
     1141 atcggccagc cgcgcggctt gctgcgtgtt gtgggggacg atcaccaccg tgcactgccg
     1201 cttcaagtcg aatagcgtct gctcgatctt gtgcgtggag accgggtcga gtgcggcggt
     1261 cggctcgtcc aggaggatga cctccggctc catggccagg acgcgggcga tggagaggcg
     1321 ttgctgctgc ccgcctgaca ggtggagcgc ggattcgtcg aggcggtcgc agacctcgtc
     1381 ccacatggcg gcgcgcacca ggctccgctc cacgatctca tcgaggcgct tgcggtcgcg
     1441 aatcccgctc atgcggggac catacgcgat gttgtcatag acgctcatgg ggagggggac
     1501 cggcaaggca tagatcatcc ccacgcgccg acgcaggccg acgacgtcca tctcgggagc
     1561 gaagatatcg cgcccatcca ggaggatgcg cccggtgtgc cgcgcatggc gcgtgttatc
     1621 caccagccgg ttcatcgagc gcagcagcgt ggtcttcccc ccgcccgccg gcccgaacac
     1681 ggccagaatc tcgttacgcc tcaccgggag agtgatgttg tgcagcacct cctgattgcc
     1741 ataggcggcg ctgaattgat ccacctgtat tttgatctgc tcatccatcg aaatggctca
     1801 actccagaac ccgaatcacc tgcgcgctga gcgtccaatt gccccgggcg gcgctttgca
     1861 cgccccgtca gattacgtgg ggcgtctccg attgcggcgg acagcccgga caatcagcca
     1921 gttggccaga acgttgatca ccagcgtggt gataatcagg atggcgccgg tcgcataggc
     1981 cttctcaagc gagatcccct cgatggccag gatgtagaag tgcacggaca tggtgcgcgc
     2041 gggcgagagc agcgaggtcg gcatgttgag ggcggtgccg gcggtgaaga ttaccgcagc
     2101 ggtctcgccg acgctgcgcc caatcgcgag gatgatcccc gtgatgatgc caggtagcgc
     2161 cgtgggcagc accacggtgg tgaccatttg ccagcgcgtg gcccccaatc caaagctgac
     2221 gtctcgatac ggatgcggca ccgtcaggat cgcctcttcc gcggtgcgga tgatggtggg
     2281 gaggatcatg atcgccagcg tgagcgcgcc cgaggcgatg gaccagccca tcttcaggta
     2341 agagacgaaa aagataaatc cgaagagacc gaagatgatg gagggaacac cggcgagggc
     2401 ctcggcgccg aatcggatgg cgcgcgtgat cgggctctcc caggtatatt cggtcagaaa
     2461 gatggccgcg ccgattccga gcggtgcggc gatcaccacg gcgcccaggg tcagtgcgat
     2521 ggtgccgacg atagtgggga ggatccctcc ctcgcggccc atcgcttccg gcgccgtcgt
     2581 cagaaagctc caggtgacgt gcggcagccc aaggcgcagg atgtaggcga ggatgaaccc
     2641 gagcaccacc agcgtgccaa gcgccgatgc ccagatcaga ccgaacgcga cgcgctgcgt
     2701 cacatggggc cacgtggagt gccgcttctc tgagatcatg acttcaccct ccggcgcgac
     2761 acggtgttgg ccgccagatt gagcaccata atg
//
LOCUS       DAOSCV010000609         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23395_length_2790_cov_3.714442, whole genome shotgun sequence.
ACCESSION   DAOSCV010000609 DAOSCV010000000
VERSION     DAOSCV010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23395_length_2790_cov_3.714442"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1214)
                     /gene="ribD"
                     /locus_tag="PLY56_13900"
     CDS             complement(117..1214)
                     /gene="ribD"
                     /locus_tag="PLY56_13900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOJ22622.1"
                     /translation="MTRDEHWMRAALRQARRAVGFTSPNPPVGAVVVRQGEVVGRGCT
                     QPPGGPHAEVMALAEAGARARGATLYVTLEPCAHFGRTPPCTNAVLQAGIARVVAALE
                     DPHDIVAGRGFARLREAGVEVSVGVCEAEARSLLQPYLKHVRTGVPYGILKCAVSLDG
                     KIATRTGDSRWVSGEEARRRVHRLRQECDAVTVGVGTVLRDDPRLNVRLPGKRRDPIR
                     VVLDSQARTPVDAALFGPGGPVIIAVTERAPRERVDALAARGAEVLMVPEEAGHVSVA
                     ALFALLGSRGVMSVLLEGGGTVAAAALQAGVIDRLLFFMAPLLIGGRDAPTPVEGEGA
                     GMMAEALRLDGLEVRRVGADLAIEGYVHRYC"
     gene            complement(1228..1659)
                     /locus_tag="PLY56_13905"
     CDS             complement(1228..1659)
                     /locus_tag="PLY56_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22623.1"
                     /translation="MAWLILGAAVALDGLVAWFLRREVPKWREDGSFALVAPLRLLIG
                     ALLMTLILLLGVGVAFVLPKPESGTPPSAEMVAYWVFITLLVGAIMLLTFIDVALVRR
                     VFQRRQDEVFADILLGRGQRRGTSAGESPAPEGENGNRAGE"
     gene            1896..>2790
                     /locus_tag="PLY56_13910"
     CDS             1896..>2790
                     /locus_tag="PLY56_13910"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOJ22624.1"
                     /translation="MRDLRSFWIAIVALSCLPGAALAGPITEFIPQAHGEVKWSEGVL
                     RAIGFGVRPKDAESPAQARLLARSAAIADAYRNMAQVINEVRVTANTTVERHLTASDL
                     LRLSVQAYIRGAQVVEEQDHPDGTYTVALQVGLFGEKGLTGLVLPSLLRVPPKVSAGS
                     PPGITLDQPSLATPAPVLASRESLASPVASLEEALLTPATDEGPFTGVIIDARGLNVQ
                     PAMSPRIYDPTGREIYGTVNVDPSFAEEVGVAGYMGTIRAAMALPRVGNHPLVIRAMG
                     TPDRFHRYLTLSAEDAQRLLAA"
BASE COUNT          476 a         1003 c          902 g          409 t
ORIGIN      
        1 cgccgttgac actgacactc tcgcccacca ctgcgtctgc caggagctcg ggcgcctcga
       61 tggtcagccg taacgctcct cccgtcagga ccgcttcctg aaccgttccg attgcctcaa
      121 caataccggt gaacatagcc ctcgattgcc aggtcggcgc cgacgcgccg cacttccagt
      181 ccatccaagc ggagcgcctc ggccatcatg ccggcgccct cgccctccac gggggtgggt
      241 gcatccctgc ccccgatgag aagcggcgcc atgaaaaaca gcagccggtc gatcacgccc
      301 gcctgcagcg cggcggcggc aaccgtcccg ccaccttcca ggagcacgct catcacgcca
      361 cggctcccca aaagtgcaaa aagagcagcg acgctgacat gccccgcttc ctccggcacc
      421 atcaacacct cggcgccacg cgcggcaagc gcgtccacgc gctctctggg cgcccgctcg
      481 gtcaccgcga tgatcaccgg gccgcccggc ccgaagagcg cggcatcgac cggcgtgcgc
      541 gcctggctat ccaggaccac gcggatcgga tcccgccgct tgccaggcag gcggacgttt
      601 agccgggggt catcgcgcag cacggtgccc acgcccacgg tcacggcgtc gcactcctgg
      661 cgcagccggt ggacccggcg gcgggcttcc tcgccgctca cccagcgcga gtcgccggtg
      721 cgcgtggcga tcttcccatc cagcgacacg gcgcacttca ggatgccata gggcacgccc
      781 gtgcgcacat gcttgaggta gggctgcaag agagagcgcg cctctgcttc gcacacgccg
      841 acgctcacct cgaccccggc ctcgcgcagg cgtgcaaaac cacggccggc gacgatatca
      901 tgcgggtctt ccagggcggc caccacgcgc gcgatcccgg cttgcagcac ggcgttcgtg
      961 caggggggcg tgcgcccgaa gtgcgcgcat ggttcgaggg tgacgtagag cgtggcgccg
     1021 cgtgcgcggg cgccggcctc ggccagggcc atcacctccg cgtgcggtcc gccaggcggc
     1081 tgcgtgcagc ctcggcccac cacttccccc tgtcgcacca ccacggcgcc caccggcggg
     1141 ttggggctgg tgaatcccac ggcgcgccgg gcctggcgca gagccgcgcg catccagtgc
     1201 tcgtcacggg tcatggcatt gaggttttca ctccccggca cggttgccgt tctccccctc
     1261 gggcgccggg gattccccgg cgctggtccc gcgccgttgc ccgcgaccca gcaagatatc
     1321 cgcgaacacc tcgtcctggc gccgctggaa gacgcggcgc accagggcaa catcgataaa
     1381 ggtgagcagc atgatcgccc caaccagcaa cgtgataaac acccaatatg ccaccatctc
     1441 cgcgctcggc ggagtgccgc tctctggttt gggaagcaca aaggcaacgc ctacaccgag
     1501 aagcaagatg agggtcatca gaagagcgcc gatgagcagg cgtaggggcg ccaccagcgc
     1561 aaagctacca tcctcgcgcc acttggggac ctctcgccgg aggaaccatg ccacgaggcc
     1621 atcgagggca acggccgcgc caaggatgag ccacgccatc gagcgacacc tccccgactc
     1681 atcttagcac aaagccatgg gagggtcaag gcgagtggca tgcaatctgc atttctactc
     1741 ctggcgtttg ccccagtgcc cggcaactgc aagaaggacc tggggaggag acggcgccga
     1801 cggcacgatt cgcgaggcgc cagtggtacc caaacgggtt gggccggcgt tccgccggcg
     1861 cgcgcagcga tcggcaatga ggaggagtcg gcctgatgag agatctgagg agcttctgga
     1921 tcgcgatagt cgcactctct tgtcttccgg gcgccgctct ggcgggcccg ataacagagt
     1981 tcatccccca agcacatggt gaggtgaagt ggagcgaagg tgtactgcgc gccatcgggt
     2041 tcggcgtgcg gcccaaggat gccgagtcgc cggcccaggc tcgcctcctc gcccgcagcg
     2101 cagcgatcgc cgatgcctac cgcaacatgg cgcaggtgat caatgaggtg cgcgtcacgg
     2161 ccaatacgac ggtcgagcgc cacctgacgg cgagcgacct gcttcgcctg agcgtgcagg
     2221 cctacatccg tggcgcccag gtggtggagg agcaggatca cccggatggc acctacacgg
     2281 tggccctcca ggtggggctt ttcggcgaga agggcctcac ggggctggtt ctcccaagcc
     2341 tgctccgcgt tccgcccaag gtgagcgctg gctcgccgcc agggatcacg ctcgatcagc
     2401 cttcgctggc cacgccggcg cccgtcttgg catccagaga gagcctcgca tcacccgtgg
     2461 catccctgga ggaggcgctg ctgacgcccg ccacggatga gggccccttc acgggggtga
     2521 tcattgatgc ccgcggcctg aacgtccagc ccgcgatgag cccccggatc tacgatccga
     2581 ccggccggga gatctatggc acggtgaacg tggatccctc cttcgcggaa gaggtgggcg
     2641 tcgccggcta catgggcacg atccgcgcgg cgatggcgct ccctcgcgtg ggtaaccacc
     2701 cgctggtcat ccgcgccatg ggaacgcctg accgtttcca ccgctacctg actttgagcg
     2761 cggaagacgc gcaacgtctg ctcgcagccg
//
LOCUS       DAOSCV010000610         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23425_length_2787_cov_2.636164, whole genome shotgun sequence.
ACCESSION   DAOSCV010000610 DAOSCV010000000
VERSION     DAOSCV010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23425_length_2787_cov_2.636164"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2787)
                     /locus_tag="PLY56_13915"
     CDS             complement(<1..>2787)
                     /locus_tag="PLY56_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22625.1"
                     /translation="VACSPIATPGVGWHWLYHVPYPMVAPRERLRVTELSLDPAGPVR
                     PGAKVRCRVTFECETRPLADYAVAVRLGQPSESARHDYELFGAVLPPAVVKTSAWAPG
                     RHTVTLDVPIPEEAPRNTPATLLVSLPTGGAGLETALPGCRADEFGLHFTIPVARGAG
                     SQLASQTGGRAEGPGFSENVIRTVRGTPMLHINGQPTAPILWGYWYGNYRRYADYSAT
                     GVKIHRPLLSGSAICAPGEEEEYYPRWFAEVDRMIQAALDVDPEIRVLPALWMDPNPQ
                     VLFDNPGEQMVSGRGQVAILRTHTFPDATQVRPTFMSHEWRRLGAEGLRRLVEHMKKQ
                     PYAPRVVGLCLFAGRAGENYYGGNELNIFTNEQGQVDAKPREQWDVGDFSAAARMVFR
                     EFLVRKYGTDEALQAAWRRPGIRFDDILSPARFRSEEICDILVSAREPATAGSLRNPM
                     EPGIGALPMDYFQCFSEAMIDTFAAWGKAVKDASDGRLLVGCYYGYALSQLYTNLPLF
                     SGHTAVAKACRTPYLDFYVAPPDYDPSRRAGGDVRTWNIIDSLRLHNKLFLYEYDNRS
                     FLAEMMPKTFSQRETVSVFRRDFAAALTHNTAFWWLDFGDGQRGARSLEWFCDPAIAD
                     LAGHTKRVYDFALTLPDRGPSSQIAIFYHGETHTAQRIQPPTIPLNVSLSRLTFFDGV
                     QRIGAPADLYNLADIPLLAKSGRLDQYKMCLFLNPFYLTEEERQSLELCKGGGRTLVW
                     LWAPGLAQVGEPLSPERVSAVTGIPGIRLREEGTEPTCRMIASDHQITAGLAPGFELA
                     PRPVPPGSVWARFGNVVAPLLYVDPAAAGPETKILGQWVLDGKPRPEMAAFCIREFTQ
                     GGKREWTSVYSAVPYLTPELMRRIARYAGVHIYRDTNDILFADRHFVAVHTGAKPATD
                     TLRLPGKTPVYDVF"
BASE COUNT          458 a          936 c          966 g          427 t
ORIGIN      
        1 cgaacacgtc gtagacgggc gtcttgcccg gcaggcggag cgtgtcggtg gccggcttcg
       61 cgccggtatg caccgctacg aagtggcggt cggcgaagag gatatcgttc gtgtcgcggt
      121 agatatgcac gcccgcatag cgcgcgatcc ggcgcatcag ctccggcgtc aggtagggca
      181 cggcgctata gaccgaggtc cactcccgtt tccctccctg agtaaactcg cggatgcaga
      241 aggcggccat ctcaggccgc ggctttccgt ccaacaccca ctgcccgagg atcttcgtct
      301 cgggtccggc tgccgcaggg tcgacgtaga ggagcggcgc gaccacgttc ccgaagcgcg
      361 cccagacgct ccccggcggc accgggcgcg gcgcgagctc gaagcctgga gccaggcccg
      421 cggtgatctg gtgatcactc gcgatcatgc ggcaggtggg ctcggtcccc tcctctcgca
      481 agcggatgcc ggggatcccg gtcaccgcgg agacgcgctc tggcgaaagc ggctccccaa
      541 cctgcgccag gccaggcgcc catagccaga cgagcgtccg gccacccccc ttgcacagct
      601 ccagcgattg ccgttcttcc tctgtgaggt agaatgggtt gaggaagagg cacatcttat
      661 actggtcgag ccggccgctc ttcgccagaa gcgggatatc ggccaggttg tagaggtccg
      721 cgggcgcgcc gattcgctgg acgccatcga agaaggtcag ccggctcagg ctgacgttga
      781 gcgggatcgt gggcggctgg atccgctgcg cggtgtgcgt ctcgccgtgg tagaagatgg
      841 caatctgtga cgaaggcccg cggtccggga gggtgagcgc gaagtcatac acgcgcttgg
      901 tgtggccggc caggtcggca atggcggggt cgcagaacca ctccagcgag cgagcgccgc
      961 gctggccatc gccgaagtcg agccaccaga acgcggtgtt gtgcgtcagc gccgcggcga
     1021 aatcgcgccg gaagaccgag accgtctcgc gctggctgaa cgtcttgggc atcatctccg
     1081 ccaggaacga gcggttgtcg tactcgtaga ggaagagctt gttgtggagg cggaggctat
     1141 cgatgatgtt ccaggtgcgc acgtcgccgc cggcgcggcg gctggggtca tagtcgggag
     1201 gcgccacgta gaaatcgagg tagggcgtcc ggcacgcctt cgccacggcg gtgtgcccgg
     1261 agaagagcgg cagattggtg tagagctgcg acaacgcgta gccgtagtag caccccacca
     1321 ggagccggcc atccgaggca tccttcaccg ctttgcccca ggcagcgaag gtgtcgatca
     1381 tcgcctcgga gaagcactgg aagtagtcca tcggcaaggc gccgataccc ggctccatcg
     1441 gattgcggag cgatccggcg gtcgcgggct cgcgcgcgct caccaggatg tcgcagatct
     1501 cctcgctccg gaagcgcgcc ggcgagagga tatcatcgaa ccggattccc gggcggcgcc
     1561 aggcggcctg cagcgcctcg tccgtgccat acttgcggac caggaactcg cgaaacacca
     1621 tccgggccgc cgccgagaaa tcgcccacgt cccactgctc gcgcggcttt gcgtccacct
     1681 ggccctgttc gttcgtaaag atgttcagct cgttgccgcc gtaatagttc tctccggcgc
     1741 ggcccgcgaa gaggcagagg cccaccaccc gtggggcata cggctgcttc ttcatgtgct
     1801 ccaccaggcg ccgcagcccc tccgcgccca ggcggcgcca ctcgtgcgac atgaacgtcg
     1861 ggcgcacctg ggtcgcgtct gggaacgtgt gcgtccggag aatcgccacc tggccccggc
     1921 cgctcaccat ctgctcgccc gggttgtcga acaacacctg cgggttaggg tccatccaga
     1981 gcgcgggcag gacccggatc tccgggtcga catcgagcgc cgcctggatc atgcggtcga
     2041 cctcggcgaa ccaccggggg taatactctt cctcttcgcc cggcgcgcag atcgccgagc
     2101 cgctcaggag cgggcggtgg atcttgacgc cggtcgcgga gtagtcggca tagcgccggt
     2161 agttgccata ccagtagccc cacaggatcg gtgccgtcgg ctggccgttg atgtgcagca
     2221 tcggcgtgcc gcgcacggtg cggatcacgt tctcggaaaa gccagggcct tccgccctgc
     2281 cccccgtctg gctggccagc tgagagccag cccccctcgc caccgggatc gtgaagtgga
     2341 gcccgaactc atccgcgcgg cagccgggaa gcgccgtctc caggccggcg ccacccgtgg
     2401 ggagcgagac aagaagggtg gccggcgtat tccgcggcgc ctcttcgggg atcggcacgt
     2461 cgagggtcac ggtgtgccgg ccgggcgccc acgcgctcgt cttcaccaca gcaggcggaa
     2521 gcacggcgcc aaacagctcg tagtcgtgcc gcgccgattc ggagggttga cccaggcgaa
     2581 ccgccaccgc gtagtcggcg agcggccgcg tctcgcactc gaaagtgaca cggcaccgga
     2641 ccttcgcgcc gggccggacg ggaccggcgg gatccaggct cagctccgtc acgcgcaggc
     2701 gctctcgggg cgcgaccatc ggatagggaa cgtggtagag ccagtgccag cccacgcccg
     2761 gcgtggcaat cggcgagcag gccaccc
//
LOCUS       DAOSCV010000611         2778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23518_length_2778_cov_3.405802, whole genome shotgun sequence.
ACCESSION   DAOSCV010000611 DAOSCV010000000
VERSION     DAOSCV010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23518_length_2778_cov_3.405802"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLY56_13920"
     CDS             complement(<1..925)
                     /locus_tag="PLY56_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HOJ22626.1"
                     /translation="MIRSLYSGVSGLQAHGTAIDVVADNIANVNTPGFKAARAQFQAL
                     VAQTLRAAGTPDAGGQNPAQVGLGVTVGAIERQMTQGSPQTTGRASDLAIEGNGYFII
                     STGAGAPRYTRNGAFDINGNRQLVEAASGAAVLGWVADANGKIDTSQPITPLTIPAEG
                     DTLARQTTKLTLSGNLDATANVGDSASTTVRLYDSQGREQYITFTMVKTAPNTWSWDA
                     TDAGGASVGTGVLTFDGEGRCTTGPATVSVPLTDGAITPMVFTTTFNVQQLAESSEVV
                     ASHQDGLPPGAYQGFSVTDNGVLIGSYSNGLT"
     gene            1208..1735
                     /gene="lepB"
                     /locus_tag="PLY56_13925"
     CDS             1208..1735
                     /gene="lepB"
                     /locus_tag="PLY56_13925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ22627.1"
                     /translation="MVRYRFLLGLAAALSLHQWCPVRPVMVLGGSMEPTLRHGQLALV
                     HRGHYTRQPPRQGDVIVFRHDNMVQVKRVHALPGQRVWEVHYRDSADTVFLLDPDALP
                     RARQILERDPTVWITARDVPPGNLYVLGDGGNASYDSRHYGTVPFSAVLGKVLLREST
                     EHAATQALDRATLRL"
     gene            1971..>2778
                     /locus_tag="PLY56_13930"
     CDS             1971..>2778
                     /locus_tag="PLY56_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ22628.1"
                     /translation="MSQYVVTFGEIMMRLNPPGNLRFTQARTFEVTYAGGEANVAASL
                     AQFGIPTQFVTRLPNNELADACVAYLRSFGVGTDFIVRGGERLGIYFLENGAAQRGSK
                     VVYDRAGSSIATIEPGMVDWDRVFENARWFHWTGITPAISEGLARVCLEGIQAARAKG
                     ITVSCDLNFRKKLWKWGKPASEVMPEMVALCDVAIGNEEDAEKVFGIKAPNVDVERGK
                     VDASAYLYVAEELKKKFPNLQRIAITLRGSISASHNTWSGVLYDGQRLYTG"
BASE COUNT          465 a          912 c          896 g          505 t
ORIGIN      
        1 gggtcagccc gttgctatag ctcccgatga ggaccccatt atcggtcacc gagaagccct
       61 gatatgcgcc gggagggaga ccatcctggt gggatgcaac cacttcggag ctctccgcca
      121 actgctgcac gttgaaagtc gtcgtaaaga ccatcggggt gatcgcgccg tcggtgagcg
      181 gcacgctcac tgtggccggc ccggtggtac accgcccctc gccatcgaag gtgagcacgc
      241 ctgttccgac acttgctccc ccggcgtccg tcgcatccca cgaccaggtg ttcggcgcag
      301 ttttcaccat ggtaaaggtg atgtattgct ctcgcccctg gctgtcgtag agccggaccg
      361 tggtgctggc actgtcgccg acgttggccg tggcatccag attcccgctg agagtcagct
      421 tagttgtctg tcgtgccagg gtatcacctt ccgccggaat ggtcaggggg gtgattggct
      481 gggaggtatc gatcttgcca ttggcatccg cgacccagcc cagaaccgca gcaccggatg
      541 cggcctccac cagctggcgg ttgccattga tgtcgaaagc gccgttgcgc gtgtagcgcg
      601 gggcgcccgc tccggtggag atgatgaaat agccgttccc ctcgatagca aggtcagagg
      661 ctcgcccggt ggtttgcggg ctgccctgcg tcatctggcg ctcgatagcc ccaaccgtta
      721 ccccaaggcc cacctgcgcc gggttctgcc cccccgcgtc gggagtgcct gctgcacgca
      781 aagtctgtgc aacgagggcc tgaaactggg cacgagccgc tttgaagccc ggtgtgttca
      841 cattggcgat gttgtcggcc accacatcga tggccgttcc atgcgcctgc aatcccgaaa
      901 ccccggaata gagcgagcga atcatcgagc tacgtcctcc tctgggcgct ttctagccgc
      961 gactggcgct gcggcaacgc ccacttccta ttcggcccgg tggcccccga aggttaagtc
     1021 agccgcccgc gatccggcac gcatcttgcc tcttccgcca cgagacctgc ccgtgtctgc
     1081 acgcgggcag ctccatcccc gggcagcggc cgtgaagcgc catgcccgcc gaatggagcg
     1141 ccccccttgc gaccggcaga tcttaggggg cgcactcccg cgtgcacccc cagggcgagg
     1201 gctcaggatg gtacgttacc gcttcctgct gggtctggcc gcggcgctat cactccatca
     1261 gtggtgcccg gtacgcccgg tgatggtgct ggggggctcc atggagccta ccctgcgcca
     1321 tggtcagctc gccctggtcc accgcggcca ctacacccgt cagcctccgc gccagggaga
     1381 cgtgatcgtc ttccgacacg acaacatggt gcaggtgaag cgcgtccatg ccttgcctgg
     1441 ccagcgcgtc tgggaggtgc attaccgcga tagcgcggat accgtcttcc tcctggaccc
     1501 cgacgcgctg ccccgcgccc ggcagatcct ggagcgcgat cccacagtgt ggattaccgc
     1561 ccgcgacgtg cctccgggca acctctacgt tctgggcgat ggcgggaatg cgtcctacga
     1621 cagccggcac tatggaacgg tgccgttctc ggcggtgctt ggcaaagtgc tcctgcggga
     1681 gagtaccgag cacgccgcca cgcaggcact cgaccgggcc accctccggc tgtagagagt
     1741 gcacgactga gcgggccgcg cgcaggatcc gcgaagcgcc gctagcggca ggagatcacc
     1801 cgctcgggat agaagcgttg gagagggtgg cgtcctgacc gccctattcc cacttgatag
     1861 cttctgcaat ccggaacgag tctgttccgc atccgggtcg cacgggccgg gcgttctctg
     1921 gatggctccg gcgacgcaat ggccgagtgc ggtgtgagga gtgatgtacc atgtcgcagt
     1981 atgtggttac cttcggcgag atcatgatgc gcctcaaccc tccggggaat ctccgcttta
     2041 cccaggcgcg caccttcgag gtcacctacg cagggggcga ggcgaacgtc gccgcgtcgc
     2101 tcgcgcagtt cggcatcccg acgcagttcg tcacccgcct tcccaacaat gaactcgcag
     2161 acgcgtgcgt ggcctacctc cgctccttcg gtgttggcac cgatttcatc gtgcgtggcg
     2221 gcgagcgcct gggcatctac ttcctggaaa acggcgcggc ccagcgaggg agcaaggttg
     2281 tttacgatcg ggctggctcg tccatcgcaa ccatcgagcc gggcatggtg gattgggacc
     2341 gcgtcttcga gaacgcgcgc tggttccatt ggaccggcat tacccccgcg atcagcgaag
     2401 ggctcgcgcg cgtctgcctg gaggggattc aggccgcgcg cgccaagggg attaccgtct
     2461 cgtgcgactt gaacttccgc aagaagctgt ggaagtgggg caagccggcg agcgaggtga
     2521 tgccggagat ggtggcgctc tgcgacgtcg ccattggcaa cgaggaggac gcggagaagg
     2581 tcttcgggat caaggccccc aacgtagatg tcgagcgcgg gaaggtggac gccagcgcgt
     2641 atctctatgt ggcagaagag ctaaaaaaga agttcccgaa cctgcagcgg atcgccatca
     2701 cgcttcgcgg gagcatcagc gccagccaca acacctggtc cggcgtgctt tatgacggcc
     2761 agcgcctcta caccggcg
//
LOCUS       DAOSCV010000612         2777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23533_length_2777_cov_3.192506, whole genome shotgun sequence.
ACCESSION   DAOSCV010000612 DAOSCV010000000
VERSION     DAOSCV010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23533_length_2777_cov_3.192506"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLY56_13935"
     CDS             complement(<1..244)
                     /locus_tag="PLY56_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ22629.1"
                     /translation="MKLAFSTLGCPDWSVERVVSTASSLGYDGVEVRGFSGRVDIDQM
                     PEFNEGLARTRALFHDAGLEICCISTSVSLSGRDREA"
     gene            complement(390..1325)
                     /locus_tag="PLY56_13940"
     CDS             complement(390..1325)
                     /locus_tag="PLY56_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ22630.1"
                     /translation="MHCVVTGAAGFIGSHLCEALLAGGHTVIGIDVFTDYYPRAQKER
                     NLAGLRGRPGFTFVEGDLREVDLPRLLEGAEWVFHQAAQAGVRASWGDYFSTYTAHNV
                     LATQMLLEAVRKMPVRKLVYASSSSIYGDAERAPTPEETIPQPVSPYGVTKLAAEHLC
                     FAYARNFNVPAVALRYFTVYGERQRPDMAFHRFLRMALSGEPVPVYGDGLQSREFTYV
                     SDVVKANLLAAEHGRPGTPYNIGGGSQVTVAEVLDLMGELLGRPVARQMLPAQPGDVR
                     RTQADITRARTELGYEPTVGLREGLRRELEWLRGT"
     gene            complement(1552..>2777)
                     /locus_tag="PLY56_13945"
     CDS             complement(1552..>2777)
                     /locus_tag="PLY56_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22631.1"
                     /translation="IGWYYVYNTDATTDMFEYVLNKSLAYGASISLQTSPQNIREHPY
                     IDQIVDLIAAYEELRLGGKVPETTLARLREMQKDYHLVQEGKQRWLQRVIYEPWRDIT
                     ALDGKTNAWEVEVKEGPCRVGVDLQVVRAAWSRPGRAYYSPQARILESFDSLAPYLED
                     PKNDLDTLVVGPGKAGWTHAGVTQELRSVAEDAREGGRCAVYTATSTLSGQGGWSCVG
                     RVFDPPLDLSWHKGIGFWLRGDGQGGKFKLQLRDGEGATDYYIDNDYEGWRYHQLPRP
                     EKDPIDYSRVRYLAFYYNGLPGKTTVTCAIDDVKALPELDEITVENPRLEIDGKPLAW
                     SVTLRDGQALSYLPGQATRLTGGGTARGTTLPSPAALTLPPGRYTVRFTTPGPLTGVS
                     RVRLTLQPEERLPVE"
BASE COUNT          452 a          940 c          912 g          473 t
ORIGIN      
        1 gcgcttcgcg atcccgcccg ctcagcgaca cgctcgtgga gatgcagcag atctccagtc
       61 cggcatcgtg gaagagcgcg cgcgtgcgtg ccaggccctc gttgaactcc ggcatctggt
      121 caatgtcgac gcgcccgctg aagccgcgca cctccacgcc gtcgtagccc agcgaggaag
      181 cggtggagac gacgcgctcc acgctccagt cggggcatcc caacgtgctg aaagccaact
      241 tcatccgtca ttcctcctgg ctgtcgcgtc tccatttccc tttcgggagc gcggcctcct
      301 gccgcgggcg gaacgcgcac tcctagacct gggcgtcgaa cgcggcgcgc catcgactgg
      361 cgggctaatg cctctgaatc cacggtccat caggtgcctc ggagccactc gagctcgcgg
      421 cgcagaccct cgcggaggcc tacagtgggc tcgtagccca gctccgtccg ggcgcgcgtg
      481 atatccgcct gggtccgccg gacgtcgccc ggctgcgccg gcagcatctg gcgtgccacc
      541 ggccggccca acagctcgcc catcagatcc agcacctccg ctacggtcac ctggctcccg
      601 ccgccgatat tgtagggcgt gcccggccgg ccgtgctcgg cagcgaggag attggccttc
      661 accacgtcgc tgacgtaggt gaactcccgg ctctgtaggc catccccata tacgggcacc
      721 ggctcgccgc tcaaagccat ccgaagaaag cggtggaacg ccatatccgg gcgctgccgc
      781 tcgccgtata ccgtgaagta gcgcaaggcc accgcgggca cgttgaagtt gcgcgcgtag
      841 gcaaagcaga ggtgttccgc cgcgagtttg gtgaccccat agggagagac cggctgcggg
      901 atcgtctcct cgggcgtggg cgcgcgctcg gcatcgccat agatggagga agaagaggcg
      961 tagaccagct ttcgcacggg cattttgcgg actgcctcga ggagcatttg ggttgccaga
     1021 acgttatgcg ccgtgtaggt gctgaaatag tcgccccagc tcgcgcgcac gcccgcctgc
     1081 gctgcctgat ggaagaccca ctcggcgcct tcaagcagcc ggggaaggtc cacctcgcgc
     1141 aggtcgcctt cgacgaaggt gaatccgggc cggccacgca ggccggcgag gttgcgctcc
     1201 ttctgcgcgc gagggtagta atccgtgaag acgtcaattc cgatgacggt gtgcccgccg
     1261 gcaagcaagg cctcgcacag gtgcgagccg atgaaaccgg cggcgccggt taccacgcag
     1321 tgcatatgag ttctcctcca gagatgagat tagccacgca cgcgcgtctt cctgtgtcgc
     1381 tcccgcccgg ctgtgaccag cgggcagtag caacaccaga cgggcagcag gatggccgcc
     1441 ggggccaccc cactgcccgc gtctgcgatg ccgcggccca gaagggctcg ctcgcgcagg
     1501 agatctcccg tcgggctctt cacatcaggc cggcggtctg acgagctcgg gctactcgac
     1561 cggtaaccgc tcttccggct ggagcgtcag ccggacgcga gagacaccgg tgagcggccc
     1621 cggggtggtg aagcgcacgg tgtagcgccc cggcggcagc gtcagcgcgg cgggcgaggg
     1681 gagtgttgtt ccccgtgccg tgccaccgcc tgtgagccgc gtggcctgtc cgggcaggta
     1741 ggagagcgcc tggccgtcgc gcagcgttac gctccaggcc agcggctttc catcgatctc
     1801 caggcgcggg ttctccaccg tgatctcatc cagctccggg agagccttca cgtcatcgat
     1861 ggcgcaggtg accgtggttt tccccggaag cccgttgtag tagaaggcga gatagcgaac
     1921 ccgcgagtag tcgatgggat ccttctccgg ccgggggagc tgatgatagc gccagccctc
     1981 atagtcgttg tcgatgtagt agtcggtggc gccctcgccg tcacggagtt ggagcttgaa
     2041 cttgccgccc tggccgtcgc cgcgcagcca gaagccgatc cccttgtgcc acgagaggtc
     2101 gagcggcggg tcgaacaccc tgccgacgca cgaccacccg ccctggccgc tgagcgtgct
     2161 ggtcgccgtg taaacggcgc agcgcccacc ttcccgggca tcctccgcca cggaacggag
     2221 ttcctgggtg accccggcgt gcgtccatcc tgccttgcca gggcctacga cgagcgtgtc
     2281 gaggtcgttc ttcggatctt ccagataggg cgccagggag tcgaagctct ccaggatgcg
     2341 cgcctgcggg gagtagtagg cgcggccagg gcgggaccag gccgcgcgca ccacctgcag
     2401 atcgacgccg acccggcagg gcccctcttt gacctccacc tcccaagcgt tggtcttccc
     2461 atcgagggcg gtgatgtcgc gccacggctc atagatcacg cgctgtaacc aacgctgctt
     2521 cccttcctga accaggtggt aatccttctg catctcgcgc agccgggcaa gcgtggtctc
     2581 cggcactttc ccgcccagcc ggagctcctc gtaggccgcg atcaggtcca caatctgatc
     2641 gatgtaggga tgctcgcgga tgttctgcgg cgacgtttgg agcgagatcg aggcgccgta
     2701 ggccagcgac ttgttcagta catactcgaa catatcggtg gtcgcgtcgg tgttatagac
     2761 gtagtaccag ccgatat
//
LOCUS       DAOSCV010000613         2775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23554_length_2775_cov_2.906985, whole genome shotgun sequence.
ACCESSION   DAOSCV010000613 DAOSCV010000000
VERSION     DAOSCV010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23554_length_2775_cov_2.906985"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1708)
                     /locus_tag="PLY56_13950"
     CDS             complement(35..1708)
                     /locus_tag="PLY56_13950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl-hydrolase"
                     /protein_id="HOJ22632.1"
                     /translation="MNESKRRRFRYTGRKTNEISFPLGGIGTGCIGLGGNGRLIDWEI
                     FNRPNKGSFNGFSHFAIKAERDGTLLDARVLNGDLHPPYTGQLNGRAYGSFGFGPPRQ
                     TLAGMPHFRSVTFQGPFPIATLSFRDESFPGRVRLTAFNPFIPLNDRDSGIPAAFFEI
                     EVRNPTGAPITYTVAGTLANPLPERNVHRIFREGNATFLVLESDGVSPEETRYGNLTL
                     ATDTEEVSGQEYWYRGAWNDNLEVYWRDFTTPGRLVNRSYPPEEAGRDNHGTLAAHVT
                     VPAGGRARVRFVISWSFPNCENYWKPNACECAREAGVSPTWRNYYATLWKDSRESAAY
                     ALSEWPRLFAETRRFRQALLASDLPAAALDAVTANLAVLKSPAVLRLEDGTFYGWEGC
                     HPSAGCCEGSCTHVWNYAQALPFLFPKLERSMRVADYRYNLRPDGGMPFRLQLPLGTP
                     SSGFRPCADGQFGGVMKAYRDWKICGDTEWLRALWPAIKASIEFAWHPENPDRWDPDQ
                     TGVLWGRQHHTLDMELFGPNSWLTGFYLGALKAGAEIAEALGEPETAAR"
     gene            1889..>2775
                     /locus_tag="PLY56_13955"
     CDS             1889..>2775
                     /locus_tag="PLY56_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22633.1"
                     /translation="MYALTPLKVYALDQVRDDSACMTRMERILSALGKTTGDLVWITD
                     ESLPDVVEELQNLWPPERVPEGQVRSFMRPIVFTRMDLSSTWPDLTPLLARCPAGTSL
                     GILKKIYGQMTTAIDQHPHHLDQRNNCVCWPTINFGTISGCPHGCQYCGAGRGGKFLT
                     VALNLEEFMEKVVGPVIEENPWNKVFRMILDGADLITFETEYGLHDLFTRKLAEYEGR
                     YGHFHTVSSNVEWMADLPHRDRLVGVWSTTCEAVARHFEPGTGAAIDRIKAARKVQEM
                     GIPVRYKFKPIIPVRNWRE"
BASE COUNT          491 a          913 c          922 g          449 t
ORIGIN      
        1 cggtccacgc cttcccctac gcgaagaggg cgcgctagcg ggcggcggtc tcgggctcgc
       61 caagcgcctc agcaatctcg gcgccggcct tcagcgcgcc caggtagaac ccggtgagcc
      121 aggagttcgg cccgaacagc tccatatcga gcgtgtggtg ttgccggccc cagaggacgc
      181 cggtctgatc cggatcccag cggtccgggt tttccgggtg ccaggcgaac tcgatcgacg
      241 ccttgatcgc cggccagagc gcgcgcagcc actccgtatc gccgcagatc ttccagtcgc
      301 ggtaggcctt catcaccccg ccgaactggc cgtcggcgca gggccgaaac cccgaagacg
      361 gcgtgccgag agggagctgg agacggaatg gcatcccgcc atcggggcgg aggttatacc
      421 ggtaatccgc cacgcgcatc gaccgctcca gcttcggaaa gaggaagggg agcgcctgcg
      481 cgtagttcca aacatgggta cacgagccct cgcagcaccc cgccgaggga tggcacccct
      541 cccacccata gaacgtgcca tcttccaggc ggaggaccgc cggcgacttc agcacggcaa
      601 ggtttgccgt caccgcgtcg agcgccgcgg caggcaggtc ggacgcgagg agcgcctggc
      661 gaaaccgcct ggtttcagcg aagagccggg gccactccga gagggcgtag gctgcactct
      721 cgcgcgagtc tttccacagg gtagcatagt agttgcgcca ggtgggcgag acgccggcct
      781 ctcgcgcgca ctcgcaggcg ttcggcttcc agtagttctc gcagttgggg aagctccagg
      841 agatgacaaa ccggacgcgc gcccgccctc ccgccggcac ggtgacatgc gcggcgagcg
      901 tgccgtggtt gtcgcgcccg gcctcctccg gggggtaact gcggttgacc agcctgccgg
      961 gcgtcgtgaa gtcgcgccag tagacctcca ggttgtcgtt ccaggcgccg cgataccaat
     1021 actcctggcc gctcacctct tccgtgtcgg tggcgagggt gaggttgccg tagcgcgtct
     1081 cctcgggcga aacgccatcg ctctccagaa cgagaaacgt ggcgttcccc tcccggaaga
     1141 tccggtgtac attgcgctcc gggagcgggt tggcgagcgt gcccgcgacg gtgtaggtaa
     1201 tgggcgcgcc ggtggggtta cgcacctcga tctcgaagaa ggccgccggg atcccggaat
     1261 cgcggtcgtt caagggaatg aaggggttga acgcggtgag ccgcacccgg ccggggaaac
     1321 tctcgtcgcg gaaggagagc gtcgcaatcg gaaacggccc ctgaaacgtc accgagcgga
     1381 aatggggcat gccggccagc gtctgccgcg gcgggccgaa gccgaacgag ccatacgccc
     1441 ggccattgag ctggccggta tagggcgggt ggagatcgcc gttgagcacg cgcgcatcca
     1501 gaagcgtgcc gtcgcgctcc gccttgatcg cgaaatgcga gaaaccgttg aagctcccct
     1561 tgttgggccg gttgaaaatc tcccagtcga tcagccgacc gttcccaccc aggccgatgc
     1621 acccggtgcc gatccctccc agcgggaagg agatttcatt ggtcttccgc cctgtgtacc
     1681 gaaaccgtcg tctcttgctc tcgttcatcg tcgccttcct cgttccggcg gctccctcgc
     1741 cagccacgcg gcgagagaag ctgttggatc gtttcccggc cggggcgcgt ttccctgcac
     1801 cggacgcctg gcgctcaagg ggcagtccac gagaggagag ttttcgccgg gcgcgaagaa
     1861 ctagctctca aggagggaga agcccgccat gtatgcactg acgccgctga aagtctacgc
     1921 cctggatcag gttcgagacg actccgcctg catgacacgg atggagcgga tcctaagcgc
     1981 gctggggaag acaaccgggg atctggtctg gatcacggat gagagcctgc cggacgtcgt
     2041 cgaggagctc cagaatctct ggccgcctga gcgcgtgccc gaggggcagg tccgctcctt
     2101 catgcgcccc atcgtcttca cgcgcatgga tctgagctct acctggcccg atctcacacc
     2161 gctcctcgcg cgctgccccg ccggcacgtc gcttgggatt ctgaaaaaga tttacgggca
     2221 gatgaccacc gccatcgacc agcacccgca ccatctcgat cagcggaaca actgtgtttg
     2281 ctggccgacg atcaacttcg gcacgatcag cgggtgcccg cacggctgcc agtattgcgg
     2341 cgcgggccgg ggcggcaagt tcctgacggt cgcgctcaac ctcgaggagt tcatggagaa
     2401 ggtggtgggc cccgtcatcg aggagaaccc ctggaacaag gtcttccgga tgatcctcga
     2461 cggcgcggat ctgatcacct tcgagacgga gtatgggctg cacgacctct tcacgcgcaa
     2521 gctcgccgag tatgaaggcc gctacgggca tttccacacg gtcagctcga acgtagagtg
     2581 gatggcggat ctcccgcacc gcgaccgcct ggtgggcgtc tggagcacca cgtgcgaggc
     2641 ggtcgcccgg cacttcgagc ccggcaccgg cgccgccatc gaccggatca aagcggcgcg
     2701 caaggtacag gagatgggca tcccggtccg ctacaagttc aagccgatca ttccggtgcg
     2761 caactggcgc gagga
//
LOCUS       DAOSCV010000614         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23626_length_2770_cov_3.303131, whole genome shotgun sequence.
ACCESSION   DAOSCV010000614 DAOSCV010000000
VERSION     DAOSCV010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23626_length_2770_cov_3.303131"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLY56_13960"
     CDS             <1..186
                     /locus_tag="PLY56_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22634.1"
                     /translation="PGEAPLPVDHDVVSRAEIVVKINTHALSPLLRACPGAPGQQLRR
                     AVNLAAYSGWFLQLWMR"
     gene            complement(451..2367)
                     /locus_tag="PLY56_13965"
     CDS             complement(451..2367)
                     /locus_tag="PLY56_13965"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22635.1"
                     /translation="MGKVSEALERLNIQHRMMLWFTAIILGVLGCVLLFLHLRITRDM
                     RNQVYSELMRTRTLFENIHRMSGEDLQSQCLILANLTRLRAAVTRQSPEEVQSVLEDY
                     ATMPGAEAVLITTPDGRPLASRGLPAPRGHRAPLYDSVRQALAGATPPGIDVSAAGIY
                     QTAWCRVRHARTGMPVGLLVLGTPIDKRLAIRLSSMSGAAITFFAPRPGSDAPVPTVV
                     ASTLPGEAMLRLQEAMLAMGRNPLSVPSEGLESPYVMDLGEEVQYLSVTAPIRGEGRK
                     VVGAYVIQRPLQEEFRLLDRMRLTLIFVGVVAVLLGLCASFIVANGVGRRIHGVVRAA
                     EGLSAGDWSRRVPIEGPKDLRVLAEAFNRMASRLQGWDAELRAEVALRTEELNQALAR
                     LDSNLRQMRQFHADASHELRTPLTIMRGEVEVALRTSRTPEEYQRVLASVLEEMNRVS
                     AIVEHLLLLARADSGQILIERAPVSIDALLEELHPQAVLLAGRKEITVTLEKEGPVMV
                     LGDSDKLRQLFLNLIDNAIKYTPEGGQVRLRVRGDSGFAVVSVEDTGIGIAPEDVPHI
                     FDRFFRADRARSREMGGTGLGLAICQWIIDGHHGWIDVQSTLGEGSCFTVYIPRVDPS
                     GPVDASAPGHPADD"
     gene            complement(2370..>2770)
                     /locus_tag="PLY56_13970"
     CDS             complement(2370..>2770)
                     /locus_tag="PLY56_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22636.1"
                     /translation="DYLTKPFAFAELLARLRALIRRGQQRGAEDRLEAFGISMDLRTH
                     RVTRDGEAVELTGKEYALLELFLRHPGQVLTRTMISEQIWGYHFDSFTNVIDVYVNYL
                     RKKLDQNGRPRLIHTVRGVGYVLRREEAEE"
BASE COUNT          452 a          923 c          951 g          444 t
ORIGIN      
        1 ccgggcgagg cgccgctccc agtggatcac gatgtcgtat cccgcgccga gatcgtcgta
       61 aagatcaaca ctcatgcgct ctctccctta ctccgggcgt gccccggcgc gccggggcag
      121 cagttacgcc gggcggtgaa tctggcagca tactccgggt ggttcctcca gctctggatg
      181 cgatgagtag acgcgcccca tcgggatcgc gggtagggga ggggaacgcg ggccgggccc
      241 cgtaagccgg gcggcggctg gggtggcgac gggataccta ccccgggtct ggctaaggtt
      301 cgatcagcgt ggagcctacc aggccgatat cgggtcacgc cgcccgatgc gtttcgggtc
      361 cgggcccgca gtagatatgc gacagttcgc atctgctgat cagtatagca agtcaacatt
      421 agaacaccat taaaaacgca ttagagtttt ctaatcatcg gcggggtggc cgggcgctga
      481 cgcatccacc ggcccagagg ggtccacgcg ggggatatag acggtgaagc aggagccctc
      541 gcccagggtg ctctggacgt cgatccagcc gtggtggccg tcgatgatcc actggcagat
      601 cgccaggccg agcccggtgc cccccatctc gcggctgcgc gcccggtcgg cgcggaagaa
      661 gcggtcgaaa atgtggggta catcctcggg ggcgattccg atcccggtgt cttccaccga
      721 gaccacggca aagccgctgt cgccgcgcac gcgcaggcga acctggccgc cctcgggcgt
      781 gtatttgatc gcgttgtcga tcaagttgag gaagagctgg cggagcttgt cggaatcgcc
      841 gaggaccatc accgggcctt ccttctcgag cgtgacggtg atctcctttc gcccggccag
      901 gagcaccgcc tgcgggtgca gctcctccag cagcgcgtct atcgagacgg gggcgcgctc
      961 aatcaggatc tgcccggaat cggcgcgcgc caggagcagg aggtgctcga cgatggcgga
     1021 gacgcggttc atctcctcca gtacgctggc cagaacgcgc tgatactctt cgggggtgcg
     1081 gctagtgcgc aacgccactt ccacctcgcc gcgcatgatc gtgagcggcg tgcgcagctc
     1141 atgcgaggca tcggcgtgga actggcgcat ctggcgtagg ttggagtcga ggcgggccag
     1201 ggcctggttt agctcctcgg tgcgcagcgc cacctcggcg cgcagctccg cgtcccagcc
     1261 ctggagccgg cttgccatcc ggttgaacgc ctccgcgagg acgcgcaggt ccttcggacc
     1321 ctcaatcggc acgcgccggc tccagtcgcc ggcgctcagg ccctcggcgg cgcgcaccac
     1381 gccgtggatg cggcggccaa cgccgttcgc cacgatgaag ctcgcgcaca gccccaagag
     1441 gacggccacc acgcccacga agatcagcgt gaggcgcatc cggtccagca gccgaaactc
     1501 ctcctggaga gggcgctgga tgacgtaggc gcccaccact ttccggccct ccccgcggat
     1561 tggggcggtg acggagaggt actgcacctc ctcgccgagg tccatcacgt aggggctctc
     1621 caggccctcc gagggcacgc tgagcgggtt gcgccccatc gccagcatcg cctcctgcag
     1681 gcggagcatc gcctcgccag gaagcgtgga cgccaccacg gtgggaactg gggcatcgct
     1741 gccgggccgg ggcgcaaaga aggtgatcgc cgcgccggac atgctactca gccggatcgc
     1801 gaggcgcttg tcgatggggg tgcccagcac caggaggccc accggcatcc cggttcgggc
     1861 gtggcgcacc cggcaccacg ccgtctggta gatccccgcg gcgctgacat cgatccccgg
     1921 aggcgtggcg cccgccagcg cctggcggac gctatcgtag aggggcgcgc ggtgtccccg
     1981 aggcgcgggc agcccccggc tggcgagagg cctgccgtct ggagtggtga tcagcaccgc
     2041 ctccgcgccg ggcatcgtgg cgtaatcctc gaggacgctc tgcacctctt cgggcgactg
     2101 gcgcgtcacc gccgcgcgta gccgcgtcag attcgcgaga atcaggcact ggctctggag
     2161 atcctcgccg ctcatccggt ggatgttctc aaagagcgtt cgcgtgcgca tcagctcgga
     2221 gtagacctgg ttgcgcatgt cgcgggtgat gcgaaggtgc aggaaaagca ggacgcagcc
     2281 caagacgccc aggatgatcg ccgtgaacca cagcatcatc cggtgctgga tgttgaggcg
     2341 ctcgagcgct tcgctcacct tgcccacgac tactcttctg cttcctcccg gcggagcacg
     2401 tagccgacgc cgcgcaccgt gtggatgagc cgcggccggc cgttctggtc gagcttcttg
     2461 cgcaggtagt tgacgtagac gtcaatcacg ttggtgaagc tgtcgaagtg gtagccccag
     2521 atctgctcgg agatcatggt gcgcgtgagc acctgccccg gatggcgcag gaacagctcg
     2581 agcagggcat actccttgcc cgtcagctcc accgcctcgc cgtcgcgggt gacgcgatgc
     2641 gtgcgcagat ccatggagat cccgaacgcc tccaggcgat cctcggcgcc gcgctgctgg
     2701 ccccggcgga tcagcgcccg gaggcgcgcc agaagctccg caaaggcgaa cggcttggtc
     2761 aggtagtcat
//
LOCUS       DAOSCV010000615         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23821_length_2754_cov_2.971471, whole genome shotgun sequence.
ACCESSION   DAOSCV010000615 DAOSCV010000000
VERSION     DAOSCV010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23821_length_2754_cov_2.971471"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLY56_13975"
     CDS             <1..766
                     /locus_tag="PLY56_13975"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ22637.1"
                     /translation="CPPDTDLHELLPTASRPVAEMWEELERRCAECGNVHLRALLNLC
                     LADSALCDRWRRAPAAVRHHHAYAGGLLEHTLGVAEVALMLAARFPRIDRDLLLTGAL
                     LHDIGKVEAYRTNAPPEMTDQGRLLGHVYPGARIVDRFIDRLEGFPSELRDRVLHLIG
                     SHHGERAHGVFEVPKTPEAIVLHFADNIDAKMNAVQIACEQAGGQAWTAPVRSLADEC
                     LFIGSASAAFAADEGISEPLEDSLGSDLFAHDDDEE"
     gene            771..1508
                     /locus_tag="PLY56_13980"
     CDS             771..1508
                     /locus_tag="PLY56_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019339012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOJ22638.1"
                     /translation="MIPDSLVQALRAARRVAVLTGAGISAESGIPTFRSAPDGLWSRY
                     DPMELVSIDGFRRNPRLVWEWHTWLRDLVKAAEPNAAHRALAKLEQHVPHCTVITQNI
                     DDLHQRAGSREVIRLHGDLFETRCSREGRLLSEAELDHTTVPPRCPCGAYARPGEVWF
                     GEMLPPGALEAAAEAMRTCDLCLVVGTSAVVQPAASLVNLAPCSAVRVVVNPERTDHA
                     RSADYLLQAPAGTALPLLVDAAFPDSA"
     gene            complement(1551..2291)
                     /locus_tag="PLY56_13985"
     CDS             complement(1551..2291)
                     /locus_tag="PLY56_13985"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOJ22639.1"
                     /translation="MMRLILYALCAALLLFGVPSAPVTAAPSITSSVNTRIHDLQATA
                     VLNEANLAALKKIGKDYADAYRAKTMESSFKEPGKLRFEAKAVGITFTQVVNGNQIHT
                     SVLGIRSTRDISTRPQSRLTTLELGFIAPSALRDYTTKYLRKQTLHNTACQVFELRYR
                     QQDQRTKKTLLYVDPAKKVLVCRELYRPDGSLKARFTYLNPKQVAPGIWLPTRITVHN
                     GDGELGGVTTYSNFRVNTGLPDSLFEKA"
     gene            complement(2443..>2754)
                     /locus_tag="PLY56_13990"
     CDS             complement(2443..>2754)
                     /locus_tag="PLY56_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22640.1"
                     /translation="WFRLTGLPFVWAVWAVRPEVKMAPLIALLRQAWEVGRNYLDAIA
                     TSTSEQLGLPLAVCRNYLYRIMKYDLGEQEWEGLRRFQALGKRHGVIPADAPPLTIVS
                     P"
BASE COUNT          445 a          888 c          925 g          496 t
ORIGIN      
        1 ctgccctcca gacaccgacc tccatgagct gctcccgacg gcgtcgcgcc cggtggcaga
       61 gatgtgggaa gagttggaac ggcgatgcgc cgagtgtggc aacgtccatc tgcgcgcgct
      121 cctgaacctc tgcctggctg actccgcact gtgcgaccgc tggcggcggg cgccggccgc
      181 tgtgcgtcac caccatgcct atgccggcgg cctcctcgag cacacgctcg gcgtggccga
      241 ggttgccctc atgctcgcgg cgcgcttccc gcggatcgac cgggatttac ttctgactgg
      301 cgccctcctg catgacattg gcaaggtgga agcctaccgg accaacgccc caccggagat
      361 gacggaccag gggcggctgt tgggccatgt ctatcccggc gcacgcatcg ttgaccgctt
      421 catcgaccgc ctcgagggat ttccatcgga gttgcgcgac cgcgtcctcc atctgatcgg
      481 gagccaccat ggcgagcggg cgcatggcgt cttcgaggtg cccaagacgc ctgaggcgat
      541 agtcctccac ttcgcggata acatcgatgc caaaatgaac gctgtccaga tcgcctgcga
      601 gcaggcaggg gggcaggcat ggaccgcgcc ggtccggtcg ctggccgatg aatgcctctt
      661 catcggaagc gcgtccgccg cgtttgccgc cgacgagggc atctccgaac cgctggagga
      721 ctctttgggc agtgatcttt ttgcccacga tgatgacgag gagtgaacgg ttgattcctg
      781 attctctggt ccaggcgctg cgcgcggcgc gtcgggtcgc cgtgctgacg ggcgccggga
      841 tctccgccga gagcggcata cccacctttc gcagcgcccc ggatggcctc tggtctcgct
      901 acgacccgat ggagttggtc tcgattgatg ggttccggcg aaaccccagg ctggtatggg
      961 agtggcacac ctggctgcgc gacctggtga aggccgccga gccaaacgcg gcgcaccgcg
     1021 cgctcgcgaa gctggagcag catgtgccgc attgcacggt gatcacgcag aatatcgacg
     1081 atctccacca gcgcgccggt agccgcgagg tgatccgcct gcatggggat ctcttcgaaa
     1141 cgcgttgctc gcgggagggc cggctccttt cggaagcaga gctggatcac accacggtgc
     1201 cgccgcgctg cccctgcggg gcgtatgcgc gccctggcga ggtgtggttc ggcgagatgc
     1261 tccccccagg ggcgctcgaa gccgcggctg aggcgatgcg cacgtgtgac ctctgcctgg
     1321 tggtgggcac gtcggccgtc gtccaaccag ccgcgagcct ggtcaacctg gccccgtgca
     1381 gcgcggtccg ggtggtggtc aacccggagc gcactgacca cgcgagaagc gctgactatc
     1441 ttctccaggc cccggcgggc acggcgttgc ccctgctcgt agacgcggca ttccccgatt
     1501 cggcctgaga ggggagcccc atccttcccc ggggcggggg aggcgagggg ctacgccttc
     1561 tcgaagagcg agtctgggag cccggtgttc acgcggaagt tgctgtaggt ggtcacgccg
     1621 cccagctcgc catcgccgtt gtggacggtg atccgtgtgg gcagccagat cccgggcgcc
     1681 acctgctttg gattgaggta agtgaaccgc gctttcagcg agccatccgg gcggtacagc
     1741 tcgcggcaca cgagcacttt cttggccgga tccacgtaga gcagtgtctt cttcgtccgc
     1801 tggtcctgct ggcggtagcg cagttcaaag acctggcatg cggtgttgtg gagcgtctgc
     1861 ttgcgcaggt acttcgtcgt gtaatcgcgc agggcgctgg gggcgatgaa gccgagttcc
     1921 agcgtcgtca ggcgggattg cgggcgcgtg ctgatatcgc gcgtcgagcg gatcccgagc
     1981 acggaggtgt ggatctgatt gccattcacc acctgcgtga aggtgattcc cacggccttg
     2041 gcttcgaagc gaagcttccc cggctccttg aagctgctct ccatggtctt ggcacgatac
     2101 gcgtcggcgt agtccttgcc aatcttcttc agcgcggcga gattcgcctc gttcagcacc
     2161 gctgtcgcct gcagatcatg gatccgggtg ttgacggacg aagtgatcga tggcgctgca
     2221 gtgaccgggg cagaagggac accgaacagg agcagcgccg cgcagagagc gtatagaatt
     2281 agtctcatca tcccacatat tcccagcctg gggcgcggct gcgccacggc gataccagat
     2341 aggggaaccg cgagcgccgt gagagtgggc gcaccgcgcg ttcccaccta ctttcaagat
     2401 accttttcgt ctctcaccgc tccatgctgg cgggcgcgtt tctcagggcg agacgatggt
     2461 caaaggaggg gcatcggccg gaatgacccc atgccgcttt ccgagcgcct ggaagcggcg
     2521 aaggccctcc cactcctgct cccccaggtc gtatttcata atgcgataca ggtagttgcg
     2581 gcagacggcc agcggcaacc ccagctgctc cgaggtgctc gtggcgatag cgtccaggta
     2641 gtttcggccc acctcccacg cctggcggag gagagcgatc agcggcgcca tcttcacctc
     2701 ggggcgcacg gcccagacgg cccagacgaa aggcaggccg gtgagccgga acca
//
LOCUS       DAOSCV010000616         2752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_23835_length_2752_cov_3.544679, whole genome shotgun sequence.
ACCESSION   DAOSCV010000616 DAOSCV010000000
VERSION     DAOSCV010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23835_length_2752_cov_3.544679"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..584
                     /locus_tag="PLY56_13995"
                     /pseudo
     CDS             462..584
                     /locus_tag="PLY56_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002018690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1880..2083
                     /locus_tag="PLY56_14000"
     CDS             1880..2083
                     /locus_tag="PLY56_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22641.1"
                     /translation="MSAHRVRIVNGKIAEVNTGSPECVVPLPAQYLGRRLDSLLYRDG
                     EWSDDPEYKEPSRPPAPAGVPPA"
     gene            2129..2689
                     /locus_tag="PLY56_14005"
     CDS             2129..2689
                     /locus_tag="PLY56_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22642.1"
                     /translation="MTRAEWVAALNTRLKDSENQLFTPEEKEAHIEAAVQEYSRRRPR
                     FFTAEIITGAGRQCYPLPEGALWVAGVVLPAPSGAPEDGCWQRGWMDDGGSLWLFPAP
                     EADGLPVRVTCAGAWAPEEVPAGETELVLLAAHARCCEVLATETARAFVFWVGDEKFD
                     KSEVSAHYRELAQALRAELRRKLARH"
BASE COUNT          417 a          822 c         1080 g          433 t
ORIGIN      
        1 cgacgtggag actcgcggtg cgccggctgt cttcctgaag gtggtctctg ccgaggcgga
       61 ggagctcgcc ctcgacgtct acgcgatgcc gtggtgagag gtccggggct cccgggggcc
      121 ttctccggag gcccccgggc gggcgagagg aggttgagat gatccgactt gccggcgcgg
      181 cgcgcgaggc catgcggggc gtggtggccg gctgggtccg ccgggttttc gcgcgcgatg
      241 ccgcccctgc gctggaggtg cgccaggatg gaagcggcga ccagctggtg atccggcgcg
      301 gcgacgagac gctcgtgcgg gtgacggcag aaggggggat cgccgggccg gtgcgcctgg
      361 tgccccaggc cgagccggag gctgccgagg aagggtacgc ttactatgac cgcgccgaaa
      421 agcgtctccg atggcatgat ggcacggcct ggcggggaag tgccgcggcg ggcgccaacg
      481 ccgatatcac ctcgctctcc gggctggtca cgccgctcga cgtggcgcaa ggtggcacag
      541 gagcgagcac ggtggccggg gcgcgcgcga agctggggat tggccgcggg gtgtttcacg
      601 ccctgggccg gccagtggga tgcgacgtgg ccgcgagcga ccctgacctg cgcgcgttct
      661 atgggggctt caccgacggc gtctatggct atctggtgcc gttcggagtg ggcgaggcgc
      721 gctcgggaaa ggtggcgcgc ctggacctgc gcgacttcgg aagcgtgacg agcctggatc
      781 tgacgactgc ggtgcgaaag ctcgcgttta ccgcgggaaa ggatgccggc atccgacgcg
      841 gcgagaccat caccggcgcg ataagcggcg cgaccgcgac cgtggcgggt gtcgcggtga
      901 cgagcgggag ctgggccacg ggagacgcgg ccggagacct ctacctgcgc gaccaaacgg
      961 ggagcttcgt cgcggagagc ctgaacgtgg ggagcgccac ggggattgcc gcggtgacgg
     1021 gcggctccgg accgtctgat ttgaccgggt tcctcggcgg gttcacggac gggcgctacg
     1081 gctacctggt gccccaaagc aactccggcg gacttcatgg ccgggcagcg cgcattgacc
     1141 tggcggactt cgccagcgtc gaggtgatcc ccctggatac gctgccgggg gagccgaagg
     1201 gcttcggagg cggcttcacg gatggagcgt atgcctactt tgtgcccacc ttcacgggtc
     1261 gactcgcgcg cgtgaacctg gtggatctcg atgagatgga agcgctcgat ctgacggcgc
     1321 tcgacggcga cctgaccggc ttcgtgggcg ggtttacgga cggtcgctac ggatacctgg
     1381 tgccgtggga tcatggggcg cgatcgggca aactggtccg cgtggacctc accgacttcg
     1441 cgagcgtggc ggtgctcaac gtggccgcgt tctgcccaga gttgaagggg ttcatcggcg
     1501 gcttcacgga tggtcgctac gcctacctgg tgccctatga taacgggcag aggttcggca
     1561 aggtggtccg ggtggatctc gccgacttct ccagcatcgc ggtgctcgac ctggaggcgg
     1621 tggatccgga tctcaagggg ttctacggcg gcttcacgga tggccgctac ggctacttcg
     1681 tgccgaacgc cgggacggtg gcaaagctgg cgcgagtgga tctggaggac ttcagcacgg
     1741 tatcggtctg cgacctgacc gcagtgcatc cccagatgcg cggctactgc ggcggctacg
     1801 tgagtggcgg ccatgcctat ctggtgccct cgttcaccgc catggtggcc cgcctgcagc
     1861 tgggctttgg aggagggcta tgagcgcgca ccgggtacgc atcgtcaacg gcaagattgc
     1921 cgaggtgaac accgggtcgc cggagtgcgt ggtgccactg ccggcgcagt atctgggccg
     1981 gcggctggac agcctccttt accgggatgg ggagtggagc gacgacccgg aatacaagga
     2041 gccaagccgg ccgccggcgc ccgcgggcgt gccgccggca tgagccaggt gaaagccggg
     2101 ccggcatccc ggcggaagga ggagcgcaat gacacgagcg gaatgggtgg cggcgctgaa
     2161 cacgcggctg aaggatagcg agaaccagct gttcacgccg gaggagaagg aagcgcacat
     2221 cgaggccgcg gtgcaggagt atagccgtcg ccgaccccgc ttcttcaccg cggagatcat
     2281 cacgggggcg ggacggcagt gctatcccct gccggagggc gcgctgtggg tggccggcgt
     2341 ggtgctgccg gcgccctccg gcgccccgga ggatggatgc tggcagcgcg gctggatgga
     2401 tgacggggga tcgctgtggc tcttccccgc tccggaggcg gatgggctgc cggtccgggt
     2461 gacctgcgcg ggcgcgtggg cgcccgagga ggtgcccgcc ggagagacgg aactggtgct
     2521 tctcgccgcg cacgcgcgct gttgcgaggt gctggcgacg gaaaccgcgc gcgccttcgt
     2581 cttctgggtg ggcgacgaga agttcgataa gagcgaggtg agcgcgcatt atcgggagct
     2641 ggcgcaggcg cttcgcgccg agctgcgccg gaaactcgcg cgccactagc ccgcattatt
     2701 catcagaata atgaaaagaa gtctctctgc caagcgtggc gcgggttcgt gg
//
LOCUS       DAOSCV010000617         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24011_length_2736_cov_2.971652, whole genome shotgun sequence.
ACCESSION   DAOSCV010000617 DAOSCV010000000
VERSION     DAOSCV010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24011_length_2736_cov_2.971652"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="PLY56_14010"
     CDS             complement(<1..1009)
                     /locus_tag="PLY56_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ22643.1"
                     /translation="MLESEQEVRETDVLVIGAGIAGLSAAVEASKGARVTVLTKASSS
                     SESATRYAQGGVAAALSPADRPELHFEDTIRAGDGLCDEEAVNVLVHEGPDRVRELIE
                     WGLQFDTENGELVFGREGAHRLHRVLHAHGDATGEMIERTLVDRAKALGAIELLKNRF
                     TLRLITHEGRCVGVHAVHEQTGEITAILARSVVLATGGLGQIYKSTTNPTVATGDGAA
                     LAFRAGAEMADLEFIQFHPTALMLEGAPPFLISEAVRGEGAILRNARGERFMLDYHEM
                     AELAPRDVVSRAIVSELRRTGTETVVLDLTHLDANYIRHRFPTISKTCESYGIDITRQ
                     PI"
     gene            complement(1139..1552)
                     /gene="folB"
                     /locus_tag="PLY56_14015"
     CDS             complement(1139..1552)
                     /gene="folB"
                     /locus_tag="PLY56_14015"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOJ22644.1"
                     /translation="MCSSDKACDRIVIRGIEFHGAHGVSEEEQAVGHRYAVDIEVITD
                     LSVAGRTDSLEDTINYAAIVRLATTIGTTMRFRLMEALAQRIADAVLEQFGPDAVHVR
                     VKKLLPPVKGVVEYAAVEICRTAPARDLTDTSARS"
     gene            complement(1901..>2736)
                     /locus_tag="PLY56_14020"
     CDS             complement(1901..>2736)
                     /locus_tag="PLY56_14020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ22645.1"
                     /translation="FGSGVEIARAIKERIRSELSLTASVGVAPNKFLAKVASDLRKPD
                     ALVVVPPEGVEEFLSTLPVSRLWGVGPATERSLRRLGIQTIGQLAAYPVETLRRRLGR
                     AGEALHRLARGKDDRPVEPSTEAKSIGRETTFPEDIGDTETLERVLLGLSEEVGARLR
                     REGVCGRTVTLKLRFSNFTTLTRSRSLPEATALAEKIYPVALALFRGVERPLKVRLLG
                     VTVSNLSPGQAARQLSLFEPPDARRERLAETVDSLRERYGDEIIRRARLVPPRHLPDG
                     S"
BASE COUNT          463 a          946 c          871 g          456 t
ORIGIN      
        1 gaatcggctg gcgtgtgata tcaataccgt aggattcgca cgtcttactg atggtgggga
       61 agcgatggcg gatgtagttc gcatccaggt gcgttaggtc gagcaccacg gtttcggtgc
      121 cggtgcggcg caactcggag acgatggcgc ggctgacgac atcgcggggc gccagctctg
      181 ccatctcgtg gtagtcaagc atgaagcgct ccccacgggc gttacgcagg atggcgccct
      241 cgcctctgac tgcttctgag atgaggaaag gcggcgctcc ctcaagcatc aacgccgtgg
      301 gatgaaactg gatgaactcc aggtcggcca tctccgcgcc cgcccggaag gcgagcgctg
      361 cgccatctcc agtggcgacc gtggggttgg tggtgctctt gtagatctgc cccagcccgc
      421 cggttgccag cacgaccgac cgggccagga tcgcggtgat ctcgcctgtc tgctcgtgga
      481 cagcatgcac gcccacgcag cgcccctcat gcgtgatcag gcgaagagtg aagcggttct
      541 tcagcaactc aatcgcgcca agcgccttgg cgcgatccac gagcgtacgc tcgatcatct
      601 cgccggtcgc gtcgccgtgt gcgtgaagca cccggtgcag ccggtgcgct ccctcgcgcc
      661 caaagaccag ctcgccgttc tccgtatcga actgcaagcc ccactcaatc agttcgcgaa
      721 cccgatccgg gccttcatga accaggacgt tgacggcctc ctcatcgcac aggccatcgc
      781 ctgcccgaat ggtatcctcg aaatggagct cgggcctatc ggcagggctc agagcagccg
      841 ccacgccgcc ttgggcgtag cgcgtggcgc tctccgatga agaagacgcc ttggtgagaa
      901 ccgtcacccg cgcccccttg gaggcttcaa ccgcggcgct aagccccgcg atccccgccc
      961 caatcaccag tacatctgtc tcgcgtacct cttgttcact ctccaacatg cgtcttcatc
     1021 cttgccaggc cgaaagaccg gcgcagcccg agcatcctcg acgcttggca gaccgccggg
     1081 gaatgaggtc acccagcact gggcggcatc gcttcttccg atgcaagagg ccgggccgct
     1141 atgaacgggc cgaagtatct gtcaggtctc gggcaggagc ggtgcggcaa atctccacgg
     1201 cggcatactc cacgacgccc ttgaccgggg ggagcagctt cttgacgcgc acgtgcaccg
     1261 catcgggccc gaactgctcc agcaccgcat ctgcgatccg ttgtgccagg gcctccatga
     1321 ggcggaagcg catggtggtg ccgatcgtgg tggccaatcg gacaatcgcg gcgtagttga
     1381 tggtgtcctc gagggagtcg gtgcgtccgg cgacgctcag gtccgtgatc acctcaatat
     1441 cgacggcata gcgatgccca acggcctgct cttcctctga taccccgtgg gcaccatgga
     1501 actcaatgcc tcggatcacg atccggtcgc atgccttatc agatgaacac attcctgtct
     1561 ccggcgcacg ccgtgccagg cggagccttc tgggcgtctc acgcccccca caacagtagc
     1621 accagtgtag cacatcgccg ggatcgagtc aatgaaaccc cgcgacgtcc cgtctgctcg
     1681 acacgcccgg gtccggtgct cacccgcgcg cggggctcga agctccgcgc tgcttctctg
     1741 caaagctctt cgggctcccc ctccctgccc ccgccgttct gagacacctg gagatgtcgc
     1801 ccaacccagc ccggcagggc ttcgctcgca agcagcgcgg tgatctatgc tgcccaagct
     1861 ggcatcatag acgcggagcg ggtgtgtgcg ccggcacggc tcaggagccg tcgggcaggt
     1921 gtcgcggggg caccaagcgc gcccggcgga tgatttcgtc accgtagcgc tcgcggagcg
     1981 aatcgaccgt ctccgccagg cgctcgcggc gtgcgtccgg cggttcgaag agcgaaagct
     2041 gccgcgccgc ctggccgggc gagaggttcg agacggtgac cccgaggaga cgcactttga
     2101 gcgggcgctc cacgccccgg aagagcgcca gcgccaccgg gtagatcttc tcggcgagcg
     2161 ccgtcgcctc gggaagcgag cgcgagcgcg tcagagtggt aaaattggag aagcgcagct
     2221 tgagcgtgac ggtgcgcccg cagacgccct cgcgccgcag acgcgcgccc acctcctcgg
     2281 agagccccag cagcacgcgc tccagcgttt ccgtgtcgcc gatatcctcg gggaaggttg
     2341 tctcgcgccc gatcgatttc gcctcggtgg atggctccac cggccggtca tccttacccc
     2401 gggccagccg gtgcagcgcc tcgccggcgc ggcccaggcg cctgcgcagc gtctccaccg
     2461 gatacgccgc cagctggccg atagtctgga tccccaggcg ccgcagcgag cgctccgtgg
     2521 ccgggcccac gccccatagc cgggagacgg ggagggtgct gaggaactcc tccacgccct
     2581 ccgggggcac cacaacgagc gcgtccggct tgcgcaggtc ggaagccacc tttgccagga
     2641 acttgttggg ggcgacgccc accgaagcgg tcagtgatag ctccgaacgt atgcgctcct
     2701 tgatcgcgcg ggcgatctct actccggatc cgaaaa
//
LOCUS       DAOSCV010000618         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24013_length_2736_cov_2.767997, whole genome shotgun sequence.
ACCESSION   DAOSCV010000618 DAOSCV010000000
VERSION     DAOSCV010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24013_length_2736_cov_2.767997"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLY56_14025"
     CDS             <1..366
                     /locus_tag="PLY56_14025"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOJ22646.1"
                     /translation="KQPQLTREQVLHSRPLRNSLLEWTDTADGKVMITIPRREDWVGK
                     MVALLFHVPKTRQLVLDDEVGSSVWRICDGEHTIKDIVEFLCKTYKLTRKEAEVSATE
                     FLRQLGRRRLIGFAVQKSD"
     gene            496..1119
                     /locus_tag="PLY56_14030"
     CDS             496..1119
                     /locus_tag="PLY56_14030"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ22647.1"
                     /translation="MASEQHATGIKRQIELPWSKAFRMSLNSIRIRFWRSMITAAGIF
                     LGIAFLQSVLTTSAILEAIGEGYDDALRLRQTWLVVMSLLVSTVGVVNSMLMSVTERY
                     REIGTMKCLGALDKLVVKLFLIEAVLTGLVASILGSLIGTMAMLLVHWIRFGSRFGVA
                     FAGAWQHLLANLAMGVTIGTVLALVAAWYPAWRAAKMPPAAALRTEV"
     gene            1227..1973
                     /locus_tag="PLY56_14035"
     CDS             1227..1973
                     /locus_tag="PLY56_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22648.1"
                     /translation="MASTAYEYIVRTKDLVKEYTMGGQTLRALNGVSLDIRRGEYISI
                     MGPSGSGKSTLFNMVGGLDKPSSGTVFIDEVDMAQLDAFEMAWLRCRKIGYIFQTFNL
                     IPVMTALENVTLPMIFAGTPTDEAIEKGIKLLQTVGLGERIHHKPTELSGGQQQRVAI
                     ARSFANDPAIILADEPTGNLDLRTGKDIIELLRRMNKDSGVTVISATHDLKMIDVSDR
                     VVWIRDGKIDKIEARADIDLKVGTMDGESH"
     gene            complement(2255..>2736)
                     /locus_tag="PLY56_14040"
     CDS             complement(2255..>2736)
                     /locus_tag="PLY56_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22649.1"
                     /translation="PSPPAEAPAATDATTTAPAASVPAPEMTEWEARAFSRLQLEPWL
                     KAHASEVRIAGLLWEPVEQRVLVTAWIPRPSELARLREEGIRIAYGVGQGLVRLGFKR
                     FEVLVKAPIPDRRGPEVVIAAEAEGAQPGLWSEDPSTADPQVLLTRFANIWWHPQLH"
BASE COUNT          487 a          895 c          848 g          506 t
ORIGIN      
        1 aagcagccgc agttgacgcg ggagcaggtg cttcattcgc ggccgttgcg caactcactg
       61 ctcgagtgga cggataccgc ggatggcaag gtgatgatca ccatcccccg ccgtgaggat
      121 tgggtgggca agatggtggc tctcttgttt cacgtgccca agacgcggca gttggtgttg
      181 gatgatgagg tgggctcttc cgtgtggcgg atttgcgacg gcgagcacac gatcaaggat
      241 attgtcgagt ttctttgcaa gacgtataag ctgacgcgca aggaggcgga ggtttccgcg
      301 acggagttcc tgcgccagct cggccggcgg cgcctgatcg gctttgccgt ccagaagagc
      361 gactagggtg agctacctca ccccagtccc ttcacgtggc gctctggcgg ccccgcggcg
      421 cccggtcttc gcttcgggca tcgagagatc tagcgcgatc cctcgggatc gcgcgcgata
      481 gggaggttgg acaagttggc tagcgagcaa cacgccactg gtatcaaacg gcagatcgag
      541 ctgccatggt ccaaagcatt ccgaatgagc ctcaacagca ttcggattcg cttttggcgc
      601 tcgatgatta cggcagccgg catcttcctc ggcatcgcgt tcctacagtc cgtgctgacg
      661 acgtcggcta tcctggaggc cattggcgag ggatacgatg atgcgctgcg gctgcgccag
      721 acgtggctcg tggtgatgtc gctcctggta tccaccgtgg gtgtcgtcaa ctccatgctg
      781 atgtcggtga cagagcgcta ccgcgagatc ggaaccatga agtgcctcgg cgcactcgac
      841 aaactcgtgg tcaagctctt cctgatcgag gcggtcctca cgggcctggt ggcctcgata
      901 ctcggttctc tcatcggaac catggcgatg cttctcgtgc actggattcg atttggctcc
      961 cgctttggcg tggccttcgc cggagcatgg cagcacctgc tggccaacct cgccatgggc
     1021 gtcactatcg ggacggtgct ggcgctcgtg gccgcgtggt atcccgcctg gcgcgctgcc
     1081 aagatgcccc cggccgcggc actccgcacc gaggtctaga gcctttttca cggcgacgca
     1141 cgcgggaacg tgcgcggtcc cccgcggagc cctttccggc ttagagccaa tggacttgcg
     1201 taagcgcgca caaggagtgg aaaactatgg catcgacagc ttatgagtat atcgtccgca
     1261 ccaaggactt ggtcaaggaa tacaccatgg gcggccagac gctgagggcg ctcaatggcg
     1321 tgtctctcga cattcggcgc ggcgagtaca tctcgatcat gggtccatcg ggatctggca
     1381 agtccaccct gttcaacatg gttggcggtc tggacaagcc atcctctggc accgtcttca
     1441 tcgacgaggt cgacatggcc cagctcgatg cctttgagat ggcctggctg cgttgccgca
     1501 aaatcggcta catcttccag acgttcaacc tgatcccggt tatgaccgcc ctggagaacg
     1561 tcaccctgcc gatgatcttc gccggcaccc ccaccgatga agccatcgag aagggcatca
     1621 agctcctcca gaccgtcggc ctcggcgagc gcatccacca caagccgacg gagctctccg
     1681 gtggtcagca gcagcgcgtg gcaatcgccc gctcctttgc caacgacccc gccatcatcc
     1741 tcgcggatga gccgaccggc aacctggacc tgcgcaccgg caaggacatc atcgagctcc
     1801 tccgccgcat gaataaggac tccggcgtca ccgtcatctc ggccacccac gacctaaaga
     1861 tgatcgacgt ctccgaccgc gtggtgtgga ttcgtgacgg caagatcgat aagatcgagg
     1921 cgcgcgcgga tatcgacctg aaggtcggca caatggacgg cgagtcccac tgaggcgcac
     1981 actcacttac gcgtaaaggc cgcgggctga agccccgcgg cctttccctt atccggcctg
     2041 ccggctatgc acggggctta tcgtccccgg aatgttttcg gccgcacatt cccatcacga
     2101 cgcgcgcatg cccttgatgc cgctcagctc ccctaccccg gcgcccgccc agggagagcg
     2161 agcagttgat tacgggattc cgaggcgcct tgcgcgcatc gaggagcaac acggtctcca
     2221 ggcgccatcc cgggacgagc cgcacggtgg agcatcagtg gagctgggga tgccaccaga
     2281 tgttcgcgaa gcgtgtgagc agcacctggg gatccgccgt tgaagggtcc tcgctccaca
     2341 gccccggctg cgcgccctcc gcttcggcgg cgatgaccac ctccgggccg cgcctgtccg
     2401 ggatgggcgc cttcaccagc acttcgaacc gcttgaaacc gaggcgtacc agcccctgcc
     2461 ccaccccata ggcgatccgg atcccctctt cccggagcct tgccagctca gaaggacgcg
     2521 ggatccacgc cgtcacgagc acccgctgct ctaccggctc ccacaagagg cccgcaatcc
     2581 ggacctcact ggcgtgggcc ttgagccacg gctccagctg caaccgcgaa aaggcgcgcg
     2641 cctcccattc ggtcatctca ggcgcgggca cgctggccgc gggggcagtc gtggtggcat
     2701 cggtcgccgc cggtgcttca gcaggcgggg atggat
//
LOCUS       DAOSCV010000619         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24032_length_2735_cov_2.777985, whole genome shotgun sequence.
ACCESSION   DAOSCV010000619 DAOSCV010000000
VERSION     DAOSCV010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24032_length_2735_cov_2.777985"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..139
                     /locus_tag="PLY56_14045"
                     /pseudo
     CDS             1..139
                     /locus_tag="PLY56_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(317..649)
                     /locus_tag="PLY56_14050"
                     /pseudo
     CDS             complement(317..649)
                     /locus_tag="PLY56_14050"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            complement(742..1605)
                     /locus_tag="PLY56_14055"
     CDS             complement(742..1605)
                     /locus_tag="PLY56_14055"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ22650.1"
                     /translation="MSGPGTPPQRVETRFGVTADAHLVGRAGPENRAHVERFTEAMRT
                     WRPDFVIDLGDILYHCGEPREEHIQELRRHWALYCSAGCAAYAVLGNHDADWLRQCSE
                     AGPGPSWQAYHEATGMPGRFYSFDVGRLHVIVLDGNAPRPPDAGASLDGHQGAFWLDE
                     LQMEWLASDLRAHRGRPKIVFCHQELHYTPPEGSGEGGQVPFPQVGSASSHVDNGWEA
                     RRLFSADGEVIACFAGHKHHPRWVVHGGVHYITLAATHRAGSYARVTVSDTLRIEGFG
                     EQPSLDLPLPA"
     gene            complement(1703..>2735)
                     /locus_tag="PLY56_14060"
     CDS             complement(1703..>2735)
                     /locus_tag="PLY56_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22651.1"
                     /translation="SRDRPAAPWGMDVALRNQKDVRVRMTMMHPLDAEVALAEGRPPG
                     GGSNAYRMPFAISKRMGDAPLRSQFLMVIEPYEGARVVERVEPMAVTPLGGAPAGEFA
                     PLGVRITTPEFVDTLILQPDASGACRTADGLVCRGEVGFWRERRNGAGQTPAAAVLAG
                     GSQLAKGAARLEVAHPVYTGRITRCDWAKRTVVVSPAPPAGMRLVGQHLQIRNDAGSH
                     VSYVVEAARPVEGGCALTLELDPRVGEGVVKSYAAGTLESQLGLRLAPWGYYAGKTLA
                     NEDGSTFYRLRDVERGNRLVLEAQVPAEGLKRDFSDRDGDGLTRYVIYDYGPGDEVIL
                     RSFATLASQ"
BASE COUNT          397 a          979 c          930 g          429 t
ORIGIN      
        1 cctgcgccag gccggctggg agatctggcg gatcgacgcc gagatgacgc tgcatgacgc
       61 cgccatgacc cgcttccgcc agtggtggcg gcgggcggcg cgggccggcc acggcttcgc
      121 gcaggtgggc gcgctgcatc ccggccagat cggcctgtcg gacatccttg agcccgcgca
      181 caatctccag gaactcgcgc gcggccggaa gggcgatccc gagtggcgtg cgtccgctgc
      241 gcccacgcag gcgctcgatg gcgttgagga tcccctcctc cgtcttcgga ccaattcgcg
      301 ggagggtgcg cagtttgtgt tcgcgcgcgg ccttctccag cttctcgatg gagtcgattc
      361 cgagctgctc gtagaacagc ttggccttct tcggcccgat cccgggcacc tccaacagct
      421 ccgccagccc ggggcggacc tgctcgcgca gatcctcgta gtaggcgagc gtgcccgtct
      481 ccagatactc cttgagtttc gccgcgatgc tctcgcccac gccggggatc tcctcgagtt
      541 caccgcgctc ggccacggcc tcaatcggct ccgcgagcgt ctcgatggcc cgggcggcct
      601 cgcggtaggc gcgtacccgg aaggggctct cgcccttgat ctccagcata tcggcgatat
      661 tctgcagcag ctctgcggcc tcggcgttct tcatcgcgca cctcccgcag aggcgtcata
      721 cccgttcgcg ggggcaagcg atcacgcggg gagggggagg tcgagcgatg gctgctcgcc
      781 aaagccttcg atgcgtagcg tgtcagaaac cgtgacccgg gcatagctgc ccgcccgatg
      841 ggtggcggcc agtgtgatgt agtgaacgcc cccatgcacc acccagcgcg gatggtgctt
      901 gtgccccgcg aaacaggcga tcacctcgcc atcggcggag aagaggcgcc gcgcctccca
      961 gccgttgtcc acgtgcgaag aggcgctccc gacctgcggg aagggcacct ggccgccttc
     1021 gccggagccc tcggggggag tgtagtggag ctcctggtgg cagaaaacga tcttcggccg
     1081 gccccggtgc gcgcgcaggt cgctggccag ccactccatc tgaagctcgt ccagccagaa
     1141 cgcgccctga tggccgtcga gagaggcgcc cgcatccggc gggcgcgggg cgttcccatc
     1201 caggacgatg acgtgaagcc tcccgacatc gaaggagtag aaccgcccgg gcattccggt
     1261 tgcctcgtga tacgcctgcc acgagggtcc gggccctgcc tctgagcatt gccggagcca
     1321 gtcggcgtcg tggttgccga ggacggcgta ggcagcgcac ccggccgagc aatagagcgc
     1381 ccagtggcgg cgcagctcct ggatgtgctc ctccctgggc tcgccgcagt ggtaaaggat
     1441 gtcgccgagg tcgatcacga aatcggggcg ccaggtgcgc atcgcctcgg tgaagcgctc
     1501 cacgtgcgcg cggttttccg gcccggcccg cccgacgaga tgggcgtcag ccgttacgcc
     1561 gaatcgcgtc tccacgcgct gcgggggcgt gccagggcca ctcacgcgca tcgctcctct
     1621 ctcgaaacag gcccgctcag gttgtggcat ggccgctcag aaggcgcgcc gggcgcctgc
     1681 gggcgcccgg cgcaccgtct tctcactggc tcgccagcgt agcaaaggag cggaggatca
     1741 cctcgtcgcc ggggccgtag tcgtagatca cgtagcgcgt caggccgtcg ccatcgcggt
     1801 cggagaagtc gcgcttcagc ccttcggctg gcacctgcgc ctccagcacg agccgattcc
     1861 ctctctccac atcgcgcagc cggtagaagg tggagccatc ctcgttggcg agggtcttcc
     1921 ccgcgtagta gccccaggga gcaaggcgca gtccgagctg gctttccagg gtgcccgcgg
     1981 catatgactt gaccacgccc tcgcccacgc ggggatcgag ctcgagggtg agcgcgcagc
     2041 cgccctccac cgggcgcgcc gcctcgacga cgtaggaaac gtggctgccg gcgtcgttgc
     2101 ggatctgcag gtgttggccc accaggcgca ttcccgcggg cggcgcgggg ctgacgacca
     2161 ccgtgcgctt tgcccagtcg caccgcgtga tccgaccggt gtagaccggg tgtgccacct
     2221 ccaggcgtgc cgctcccttc gccagctggc tgcctccggc gaggaccgca gcagcaggcg
     2281 tctggccggc cccattccgg cgctcgcgcc agaagccaac ctcgcctcgg cagaccagac
     2341 catccgccgt ccggcacgcg ccggacgcgt ccggctgcaa gatgagcgtg tctacgaact
     2401 cgggcgtcgt gatgcgcacc ccgagcgggg cgaactcgcc cgccggggcg ccccccagag
     2461 gagtgaccgc catcggctca acgcgctcta cgacgcgcgc cccctcgtac ggctcgatca
     2521 ccatcaggaa ctggcttctc agcggggcgt cgcccatccg cttcgagata gcgaacggca
     2581 ttcgataggc gttgctcccg cctcccgggg gccggccctc ggcgagcgcc acttcggcat
     2641 cgagggggtg catcatcgtc atgcgcacgc gcacgtcttt ctgattgcgc agggccacat
     2701 ccatgcccca gggcgcggcg ggacggtcgc gcgag
//
LOCUS       DAOSCV010000620         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24099_length_2729_cov_3.166043, whole genome shotgun sequence.
ACCESSION   DAOSCV010000620 DAOSCV010000000
VERSION     DAOSCV010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24099_length_2729_cov_3.166043"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLY56_14065"
     CDS             complement(<1..410)
                     /locus_tag="PLY56_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ22652.1"
                     /translation="MPQDRIIVRGARQHNLKNINVEIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDYIEGLSPAVSIDQKSTSRNPRSTVAT
                     VTEVYDYLRLLFARIGIPHCPKCGQVIASQTVEQ"
     gene            complement(653..2653)
                     /locus_tag="PLY56_14070"
     CDS             complement(653..2653)
                     /locus_tag="PLY56_14070"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ22653.1"
                     /translation="MGKSTEVSAAEIQRLEAIAAELRAYNILSIYAAQSGHPGGTLSI
                     LDIATALYFHVLNHRPEDPEWEDRDRVVWSGGHKAPALYAVLGRAGYFPLDDVMLLRQ
                     FGSPMQGHPNRLECPGVELSTGSLGQGLGASVGIALAAKLDGKAYRVYCIMGDGEQQE
                     GSVWEAAMSAGHYKLDNLVAIIDKNRLQIDGPVCEVMEIEPLADKYRAFGWHVIETDG
                     HDMRAILEAFAAAKEVQGKPVCIIADTVKGKGISFMENEAGWHGIPPKKADFERALAE
                     LQAACPSLTDAKVRQLLAKAEDYAAKVETETSAGLPTFSRSYWWNSESGMKVEMDPTR
                     FGFGRGLEKAGEDPRVVTLHADISASIKITDFEANHPERANRVFSVGIAEQNMISVAA
                     GLAKEGKIPVTGTYGVFGAGRCWDQIRTTVCYGEYNVKIAGAHGGISVGQDGPTHQAL
                     EEISLMAILPKMTLLVPCDSVETEKATHVGILDVVGPTYIRYAREATPIVTGPETPFV
                     LGKANVIRYRGAAAKFADAFETCLASDYQNENEDLAIIACGPMVPEAMRAAYILKEEY
                     GLETRVVNVHTVKPIDREAIVRAAEETGIVLTAEEHQTGGFGGLVAPVILQECARSVK
                     MEMVGVPDTFGKSGLPWQLMKYYGLTAEHIAQRAKALCDQRD"
BASE COUNT          474 a          896 c          849 g          510 t
ORIGIN      
        1 atctgctcca cggtctgcga cgcgatcacc tgcccgcact ttgggcagtg cgggatgccg
       61 atccgcgcga acaggaggcg caggtagtca tacacctcgg tcaccgtggc aaccgttgac
      121 cgcgggttgc ggctcgtcga tttctggtcg atggataccg ccggagagag gccctcgatg
      181 tagtccacat ccggcttctc catctgcccg aggaattgac gcgcgtaggc cgagagcgac
      241 tcgacgtaac gccgctggcc ctcggcatag atggtgtcaa aggcaagcga tgatttcccg
      301 gagccggaga ggccggtgat cacgaccaac ttgtctcgcg gaatctcaac gttgatgttc
      361 ttgaggttgt gctggcgcgc gccgcgcacg atgatccgat cttgaggcat agtcagtacc
      421 aaaacgcatg tttgcttcga ggcggcaaaa aaacaagtgc cgtcaggtgg cacttgatcc
      481 tatacctcag tatagcagtc tgaaaagtga gtgtcaaccg gaaattcggt gagaacccac
      541 ccgaggagga aacgtctccg cggccggcgc gcgggcgaga agggcccggt ggggcatccc
      601 gtccgcgttc ccccgtgggc cggcccgtgg cacgggccgg cccggatcgg gcttagtctc
      661 gctggtcgca caacgccttg gcgcgctgcg cgatatgctc cgccgtaagc ccgtagtact
      721 tcatcagctg ccagggcagg cccgacttgc cgaaggtgtc gggaacgccc accatctcca
      781 tcttcaccga gcgagcgcac tcctggagga tcaccggtgc taccaggccg ccgaagccac
      841 cggtctggtg ctcctcggcg gtgaggacga tgcccgtctc ctcggccgcg cgcacgatcg
      901 cctcccggtc aatcggcttc acggtgtgga cgttgacgac gcgcgtctcg aggccatact
      961 cctctttcag gatgtaggcg gcgcgcatcg cctccggcac catcggtccg caggcgatga
     1021 tcgccaggtc ctcattctca ttctggtagt cggacgccag gcacgtctcg aacgcgtcgg
     1081 cgaacttcgc cgcggcgccc cgataccgga tcacgttggc ctttcccagc acgaagggcg
     1141 tctccgggcc ggtgacgatt ggggtcgcct cacgggcata ccgaatgtag gtggggccaa
     1201 ccacgtccag gatgccgacg tgggtcgcct tctccgtctc cacagagtcg cagggcacca
     1261 gcagcgtcat cttcgggaga atggccatga ggctgatctc ctccagcgcc tggtgcgtcg
     1321 ggccatcctg gccgacagag ataccgccgt gcgcgccggc aatcttcacg ttgtactcgc
     1381 cgtagcagac cgtggtgcgg atctggtccc agcagcgccc ggcgccaaag acgccatagg
     1441 tgccggtcac cgggatcttg ccctccttgg cgaggccagc agccacggag atcatattct
     1501 gctcggcgat accgacgctg aagacgcggt tcgcgcgctc gggatggttc gcctcgaagt
     1561 cggtaatctt gatggatgca gagatgtctg catggagggt gaccacgcgg gggtcctcgc
     1621 cggccttctc gaggccgcgt ccgaagccga agcgcgtcgg atccatctcc accttcatgc
     1681 cgctctcgct attccaccag tagctgcgag agaaggtggg cagcccggca ctcgtctctg
     1741 tctccacctt cgcggcatag tcctccgcct tcgccagaag ctggcgcacc ttggcgtcgg
     1801 tgagcgaggg gcaagcggcc tgcagctccg caagcgcgcg ctcaaagtcg gccttcttgg
     1861 gcgggatgcc atgccacccc gcctcattct ccatgaagga gatccccttc cccttcacgg
     1921 tgtcggcgat gatgcagact ggcttcccct gaacctcctt cgcggccgcg aacgcttcga
     1981 ggatcgcgcg catatcgtgg ccgtccgtct cgataacgtg ccacccgaaa gcacgatact
     2041 tgtcggccag tggctcgatc tccatcactt cgcacaccgg gccgtcgatc tgcagccggt
     2101 tcttgtcgat gatcgccacc aggttgtcga gcttgtaatg gccggcggac atcgccgctt
     2161 cccagacgct cccctcctgc tgctcgccat cgcccatgat gcagtagacg cggtatgcct
     2221 tgccatccag cttcgcggcg agggcaatgc cgaccgaagc cccaagtccc tggcccaacg
     2281 aaccggtgga aagctcgacg cccgggcact ccaggcggtt gggatggcct tgcatcggcg
     2341 agccaaactg ccggaggagc atcacgtcat ccagtgggaa gtagccggcc cggccgagca
     2401 ccgcgtagag cgcgggggcc ttgtgcccgc cggaccagac aacgcggtcg cggtcctccc
     2461 actcggggtc ttcaggtcgg tggttcaaga catgaaagta gagcgccgtg gcgatatcca
     2521 ggatcgagag ggtgccacct gggtgtcccg attgtgcagc gtagatcgag aggatattgt
     2581 aagcacgcaa ctcggcggca atcgcctcca ggcgctggat ctcagcagcg gaaacctcgg
     2641 tcgatttgcc catcttctcc acgtttcgtt cctcccatcc ataagagcgc ttctgcactt
     2701 cggcgatgct tgctgccgta ccagggggc
//
LOCUS       DAOSCV010000621         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24170_length_2722_cov_3.383577, whole genome shotgun sequence.
ACCESSION   DAOSCV010000621 DAOSCV010000000
VERSION     DAOSCV010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24170_length_2722_cov_3.383577"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLY56_14075"
     CDS             complement(<1..180)
                     /locus_tag="PLY56_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22654.1"
                     /translation="MLRQRMHRVRSRLGKWAWGLAAVSLAVTASVPARASLTARFDSE
                     VSEVQRHEATDLIWGN"
     gene            complement(384..1112)
                     /locus_tag="PLY56_14080"
     CDS             complement(384..1112)
                     /locus_tag="PLY56_14080"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ22655.1"
                     /translation="MVIMAVSDLHGELSWLPAALDRIRPQLLLCAGDWGDPGQLREQE
                     FMLLLERAHVLSVYGNHDDRPLLARLKNADGTPVLLAQGETRRVLGLTVAGISGIWAK
                     TKLGSRLNRQWEAARRRKPTLVLDEWLAGRELPPYVTDEEVEALAARIAGQEIDILVT
                     HACPAGWGDRTPGGGHGGQRCFRQASAAIRPSIHLCGHLHRLQRSDLPDGSAVLNTGH
                     GAMREGWQIRWDDGRWDASPLEIP"
     gene            1465..>2722
                     /locus_tag="PLY56_14085"
     CDS             1465..>2722
                     /locus_tag="PLY56_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22656.1"
                     /translation="MRKRYSCLLCWLLLAVSLVPARASERAVLLWQDTLPGGADVASL
                     GAVLRNADIPTEVVSGRDLSAPGRLDIKRVSLLILPYGSRYPAPARDAILAYLRGGGA
                     LLTLGGRAFTEPLQPAGDGWATAEALRQPLPGDPLVVADFEPNGASPEATTASDRSDM
                     PVIRTVTEKGGHVLEAHSPSLTGFEYVLLRVHPARADRPILRFRARGDGATPFLCVEA
                     GEADGSRWKAIVPLTKKWETYRIHATEFVAYASPSRMGEGDSFHPEALRELRFGYTQA
                     MVGTGAHRFWIDDIVWEPGVTVARAGKARPLVRTAASPVEVFFGSHLTGGTGVMPLFS
                     LSTRIPDGARLVPVHPLAPPVPQRPARGWTATGWADDLFTAWLDNPGGRLSVARQSTG
                     RRETLVEARAAGGKVVGPAFSLIVQTR"
BASE COUNT          427 a          959 c          891 g          445 t
ORIGIN      
        1 attcccccag atcaggtcgg tcgcctcgtg gcgctgcacc tcggagacct cactatcgaa
       61 ccgggcagtc agcgacgcgc gtgccggaac cgaggccgtg acggccaggc tcacagcggc
      121 caggccccat gcccacttcc caagccggct ccgtactcta tgcatccgtt gccgaagcat
      181 cgctcgtcag gagactcctc acccctcgcc acaccccatg ctcacttcta ttagagggtg
      241 gatgcttcca ggaagtttcc atcgggcagg cgtgcgcgac cgcgcctggc cttatccatg
      301 gggacagggg gatgatcgtt tccgggagtc tcggttggcc ggcgccaggg agcgacccgg
      361 cttgcaggcc gcgcctgacc acgctatggg atctccagcg gagaagcatc ccagcgcccg
      421 tcatcccatc gaatctgcca tccctcgcgc atcgcaccgt gacccgtgtt caggaccgcg
      481 ctcccatccg ggaggtcgct ccgttggaga cggtgaaggt gcccgcagag atgaatgctc
      541 gggcggatgg cggcggaggc ctggcggaaa cagcgttggc ccccatggcc gccgccaggg
      601 gtccggtcgc cccatccggc agggcaggca tgggtgacca gaatatcgat ctcctgcccg
      661 gcaatgcgtg cggcgagggc ttccacttcc tcatcggtga cgtatggggg caactcgcgc
      721 ccggcgagcc attcgtcgag gacgagcgtg ggcttgcgac gcctggctgc ttcccactgc
      781 cggttaagcc gcgagcccag cttcgtcttg gcccagatcc cgctgatccc cgctacagtg
      841 agcccgagga cacggcgggt ctcgccctga gccagcaaca cgggcgtgcc atccgcgttt
      901 ttcagtctgg caaggagagg ccggtcatcg tggtttccat acaccgagag cacgtgggcg
      961 cgctcgagaa gcagcatgaa ctcctgttcg cgcaactggc ccgggtcgcc ccaatcgccc
     1021 gcgcagagga ggagttgcgg gcgaatgcgg tccagggcgg ccggcagcca ggagagctcg
     1081 ccatgcagat cgctgaccgc catgattacc atcgccatcc tccatcctga cgcatccatt
     1141 cgcccggctc gcgaggcaag cgtgcgtgtg gcactcgcca ttgcaagcca cgaaagcccc
     1201 tcgtcccccg cggttccgga aagcccggca tcactcccgc acttcccgtg ccagggttct
     1261 ccgtgtgaac acctggccgc gataggcatc ccatccactc gcgcgagcgg ggaatctcac
     1321 ccggcagccc catgaacgcg cgcgtgacac gccgcgctgg ggcaaccgct cgccgccacc
     1381 acatggaagg aacaggcgcc ggcatccgcg aacgagggtg caaccgggct ggcgccaagg
     1441 gccagtcgcc gaagggggtt ctcaatgcgc aagcgatact cctgtctcct ttgctggctt
     1501 cttctcgcgg tttcgctcgt gccggcacgc gcaagcgagc gcgccgtgct cctctggcag
     1561 gatacactgc cgggaggcgc tgatgtggca tcgctggggg cggtgctccg taacgccgac
     1621 atcccgaccg aagtcgtgag cggcagggat ctttccgcgc caggccgcct ggatatcaag
     1681 cgcgtatcgc tcctgattct accctacgga tcgcgatacc ctgcgcccgc gcgcgatgcc
     1741 atcctcgcct acctgcgcgg cggcggggcg cttctgacgc tgggcgggcg tgccttcacg
     1801 gagccacttc agcccgcggg ggatggttgg gccaccgccg aggcgctgcg ccagccactg
     1861 cccggggatc cgctggtcgt ggccgatttc gagccgaacg gggcaagccc cgaggcgacc
     1921 accgcctccg accggtccga catgccggtc atccgcaccg tgacggagaa gggcgggcac
     1981 gttctggagg cccactcccc ctccctcacc ggcttcgaat atgtcttgct gcgcgttcat
     2041 ccggcaagag cggaccggcc catcctgcgc ttccgcgcgc gcggcgatgg agcgacgccc
     2101 ttcctctgcg ttgaggcggg cgaagcggat ggctcccgct ggaaagcgat cgtgcccctc
     2161 accaagaagt gggagaccta ccgcatccat gcaacggagt ttgtggccta tgcgagcccg
     2221 tcgcgcatgg gcgagggcga ctcgtttcac ccggaagccc tccgcgagct gcgcttcggc
     2281 tacacgcaag cgatggtggg caccggcgcc caccgcttct ggatcgatga tatcgtctgg
     2341 gagccagggg ttaccgttgc tcgcgcgggc aaagcgcgcc cgctggtgcg cacggcggca
     2401 tcgcccgtgg aggtcttctt cggaagtcac ctcacgggtg gcaccggtgt catgcccctc
     2461 ttctccctga gcacgcgcat cccagacggc gcccggctgg tgcccgtgca tcccctcgcg
     2521 ccgccggtgc cacagaggcc cgcgcgcggc tggacggcca ccggatgggc cgacgatctc
     2581 tttacggcgt ggctggataa ccctggcggc cggctctccg tagcgcgcca gtccaccgga
     2641 aggcgggaga cactggttga ggcgcgcgcc gccgggggta aggtggtagg acccgcgttc
     2701 tcattgatcg tgcagacccg cg
//
LOCUS       DAOSCV010000622         2722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24172_length_2722_cov_3.089989, whole genome shotgun sequence.
ACCESSION   DAOSCV010000622 DAOSCV010000000
VERSION     DAOSCV010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24172_length_2722_cov_3.089989"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLY56_14090"
     CDS             complement(<1..387)
                     /locus_tag="PLY56_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ22657.1"
                     /translation="MAVDPATGAGDALLVERAQAGDVDAFRVLFERYHRRIYGLVLGI
                     VKSTADAEDVTQDAFVRAYRALGSLQQGHAFVPWLYRIATNLARNHLRDRRAGLWESL
                     DEGVTWDDETLEKQYADPAPSPADVVE"
     gene            591..1493
                     /locus_tag="PLY56_14095"
     CDS             591..1493
                     /locus_tag="PLY56_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOJ22658.1"
                     /translation="MQYHIPLGEGRPLRLDYTLDSGQVFRWRREEDGRWYGTIRGTGL
                     ALLQEGSHLVAEAEGEPITSGEVVRFLGLDDPLSEIRAFLSRDPVLARAFAAVYGLRL
                     VRQDPWEGLVAFICSSHNAVFRIQRMMGCLAERFGRRLRVAGRTVHTFPPARVIASLE
                     LADLAECRLGYRDAYVLEAARMVARGEIDPYALVDAPAEEARTALMKVPGVGPKVSDC
                     ILLLALGKKECTFPVDVWVRRAVLRHYRDAVRRATGVELATEEKGLTMRHYQAIREWA
                     QQAFAPYVGYAQTYLFLATRLGAI"
     gene            complement(1629..1704)
                     /locus_tag="PLY56_14100"
     tRNA            complement(1629..1704)
                     /locus_tag="PLY56_14100"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Ala,seq:cgc)
     gene            complement(1743..2168)
                     /locus_tag="PLY56_14105"
     CDS             complement(1743..2168)
                     /locus_tag="PLY56_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22659.1"
                     /translation="MPERVILAVDPGSHKCGVAVVDSSRRVRMRGVVETGELVDCVRE
                     LLEAHAITEIAVGDRTGSRRVREQLWALAGGRPVVPVDEHLSTLEARRRYFQENPPRG
                     LRRLIPLGLQVPPCPIDDLVAVLLAERCLAASERAQNQG"
     gene            complement(2161..>2722)
                     /locus_tag="PLY56_14110"
     CDS             complement(2161..>2722)
                     /locus_tag="PLY56_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22660.1"
                     /translation="ADGGKHRAQQVFFGMDALDAVTEEISRANTGVVALLRVASNTVA
                     GEPVLAEVQLWQNRLVFHEGEPIARREMNGRQQTGALVEELVAMLQTEVRATAIARGM
                     IPRDATPGVVERPRVGEVPLAELVAVAEQVRRHRGMAIVEAISAADLWTADTVRVSFR
                     VTRDLPRQRLARVAPCSRFSEVRASA"
BASE COUNT          448 a          956 c          874 g          444 t
ORIGIN      
        1 ctccaccacg tcggcgggac tgggcgccgg atcggcatac tgcttctcca gcgtctcatc
       61 atcccaagtc accccttcgt ctagcgactc ccacaggccg gcacgcctgt cgcgcaggtg
      121 attgcgcgcc aggttggtgg caatgcgata gagccacgga acgaaggcat ggccctgctg
      181 caaggaaccc aacgcgcgat acgcgcgcac aaaggcatcc tgcgtcacat cctcggcatc
      241 cgccgttgat ttgacgatcc caagcaccag gccgtagatg cgccggtggt atcgctcgaa
      301 gagcacccga aaggcatcta cgtccccggc ctgcgctcgc tcgacgagga gggcatcgcc
      361 agcacccgtc gcgggatcta cagccacctg gtgctctcct gtcttgcacc taccggagaa
      421 gacaccggtg gtgcaggtgg ggttccgcgc gtgttcagca tagcctcatt atagcagatc
      481 tagcgctggc caggtgcaaa tcgcccacaa tcgtggaaaa atggagaggt gggcaggccg
      541 cgtgaggctg gcggggcttc gcccctgccg caggggaacg gatagacacc atgcaatacc
      601 acattccact gggagagggg cggccccttc gcctggatta caccctcgac tccggccagg
      661 tgtttcgctg gcggcgggag gaggatggcc gctggtatgg cacgattcgc ggcaccggcc
      721 tcgccctcct gcaggaggga agccacctcg tcgctgaagc cgagggcgag ccgatcacgt
      781 cgggtgaagt ggtccgcttc ctgggcctgg acgacccgct ctcggagatc cgcgccttcc
      841 tatcgcgcga tccggtgctc gcgcgcgctt tcgccgcggt gtatgggctg cgcctggtcc
      901 ggcaagaccc ctgggagggg ctggtggcgt tcatctgctc cagccacaac gccgtgttcc
      961 gcatccagcg gatgatgggt tgtctggccg agcgttttgg ccggaggctg cgcgtcgctg
     1021 gccggacggt ccacaccttc ccgccggcgc gcgtgatcgc ttcgctggag ctcgcggatc
     1081 tggcggaatg ccgcctgggt taccgcgacg cctacgtgct agaggcagcg cggatggtag
     1141 cccggggcga gatcgacccg tacgcgctcg tggatgcgcc ggcggaggag gcgcgcaccg
     1201 ctttgatgaa agtgccgggc gtcggtccga aggtgagcga ctgcatcctg ctgctggcgc
     1261 tcgggaagaa ggagtgcacc tttccggtgg acgtgtgggt gcgccgcgcc gtactgaggc
     1321 actaccgcga cgcggtgcgc cgcgccaccg gggtcgagct ggcgaccgag gaaaaggggc
     1381 tgacaatgcg ccactaccag gcgattcgcg agtgggccca gcaggcgttc gcaccctatg
     1441 tcggctacgc gcaaacctac cttttcctcg ccacccgcct cggcgccatc tagtcgtttc
     1501 ggcccgaccg cccgtgcatg cgccttgagg ccgtcctgca ccagcgcccg ctgcgatgcc
     1561 caacgctccc gcaaacagaa aaagccctcc gagagggtcg tcccggaggg cttcacgttt
     1621 ctagaatttg gtggagctgg ggggactcga acccccgacc tcttgagtgc gattcaagcg
     1681 ctctcccaac tgagccacag ccccatcagc atcgttagta tatcatgaac acccggctga
     1741 tgtcaaccct gattctgtgc ccgttcgctg gcggccaggc atcgctccgc gaggaggacc
     1801 gcgaccagat catcgatggg gcaggggggt acctgcaacc ccaacgggat caatcggcgc
     1861 aggccgcgag gcgggttctc ctgaaagtag cgccggcgcg cttccagtgt ggagaggtgc
     1921 tcgtccaccg gcaccaccgg gcgccccccg gcgagggccc aaagctgctc gcgcacgcgc
     1981 cgcgaaccgg tgcggtcacc cacggcgatc tccgtgatgg catgggcttc cagcagctcg
     2041 cggacgcagt cgaccaactc tccggtctcc accacgccgc gcatgcgcac gcgccgcgag
     2101 gagtccacca ccgccacgcc gcacttgtgg ctgcctggat cgacggccag gatcacgcgt
     2161 tcaggcactc gccctcacct cactgaaccg tgagcacgga gccacccttg cgaggcgttg
     2221 tctcggcagg tcgcgggtga cgcggaagct gacccgcaca gtgtccgccg tccagagatc
     2281 cgccgccgaa atcgcctcca cgatcgccat gccgcgatgc cggcgcacct gctcggcgac
     2341 ggcaacaagc tcggcgagtg gcacctcgcc cacgcgcggc cgctccacga cgccgggcgt
     2401 cgcatcgcgc gggatcatgc ctcgcgcaat cgctgtggcg cggacctccg tctggagcat
     2461 ggccacgagc tcttccacca gcgctcccgt ctgctgccgg ccgttcatct ccctgcgcgc
     2521 gatcggctcg ccctcatgga agacgagccg gttttgccac agctgcacct ccgcgagcac
     2581 cggctcgccg gccaccgtgt tgctggcaac ccgaagcagc gcaaccacgc cggtattcgc
     2641 ccggctaatc tcctccgtca ccgcgtccag cgcatccatg ccgaagaaga cctgctgcgc
     2701 gcgatgcttc ccgccatccg cg
//
LOCUS       DAOSCV010000623         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24274_length_2715_cov_3.786090, whole genome shotgun sequence.
ACCESSION   DAOSCV010000623 DAOSCV010000000
VERSION     DAOSCV010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24274_length_2715_cov_3.786090"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLY56_14115"
     CDS             <1..305
                     /locus_tag="PLY56_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ22661.1"
                     /translation="GCLSIPGLQGNVERPERVVVRGLDVDGNEVRVSGEGYLARCLCH
                     EIDHLEGILFIDRADPTSLQWVTATEEDEVDEDGEEIGVAASAEQPRRRTRALRRR"
     gene            315..>809
                     /locus_tag="PLY56_14120"
     CDS             315..>809
                     /locus_tag="PLY56_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018756542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ22662.1"
                     /translation="MRVVYMGTPEFAVPALQALHGAGYEIALVISQPDRPRGRGRQAN
                     PTPVAATARELGLPLLQPAKVNDPEVVEQVQELAPDAIVVAAFGQILRKPLLQIPAIG
                     CVNIHASLLPQYRGGSPVQHALLAGERVTGITIMLMDEGMDTGPILMQREVLISPEDT
                     AGTLL"
     assembly_gap    810..819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <820..1254
                     /locus_tag="PLY56_14125"
     CDS             <820..1254
                     /locus_tag="PLY56_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ22663.1"
                     /translation="DRLARVGADLLLEALPALAAGRLEPTPQDASRATYAPNLSREDA
                     RIDWSSSAEKIRNQVRAFSPKPGAWAEFRGKEVKIWRARVDSDATDASPGQIVAIGPE
                     SIRVATGEGTLWLEEVQEAGKSRMGAGAFARGARLAPGERFS"
     gene            complement(1314..>2715)
                     /locus_tag="PLY56_14130"
     CDS             complement(1314..>2715)
                     /locus_tag="PLY56_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22664.1"
                     /translation="AAERIQTAQRMLESLTGATHQTFPRELQERSWRADEALRNGLRS
                     TGAERRAYHQNALSLLRRVVDDRAGRRDHAAWFQLGWLAWKHAQRLHEAEEAFAQCAR
                     LTTVTRDLFYVESLRHVAHIRYLRGNIVGAYRAIADALQVSLDPEVLFDGARYAALTG
                     RISEAQEWIAQCIQRSPAVAISALAEADFTALARELHELISRVLSQARYQARTMVTHW
                     SDALDRIASWQEQSGHTVELPTYLGRSCADAAASEVTDAGYLTVLEIERYAARASAPA
                     LAFATRELEEEACRRRDATAALKEELENAIHEAGWESSPEVRQKEAEAEAAERECKAL
                     LEAPPPLEEHALAGCGIMLAGAAFLGGLAALHGEIAAVLRTPVGLLLSAVMLGGWAFI
                     PILRWMRYRMQADAVMEKALAARRAAEELQARIIAETPSELSERIESLRQQLQEAEAG
                     KARMDELLATIKESNR"
BASE COUNT          427 a          873 c          938 g          467 t
ORIGIN      
        1 agggctgcct gagcatcccc gggcttcagg gcaacgtgga gcgccccgag cgcgtcgtcg
       61 tccgtggcct cgatgtcgac ggaaacgaag tgcgtgtgag tggcgagggc tatctggcgc
      121 gctgcctctg ccacgagatc gaccatcttg agggcattct cttcatcgac cgggcggatc
      181 ccacttcgtt gcagtgggtg acggcaactg aggaagatga ggtggatgag gatggcgagg
      241 agatcggggt cgcggcctct gcggaacagc ctcgccggag gacgcgggct ctgcgccgtc
      301 gctgaggtga tctgatgcgg gtggtttata tgggcacgcc ggagttcgcc gtaccggcac
      361 tgcaagccct ccatggcgcc ggctacgaga tcgcgctggt gatctcgcag ccggaccggc
      421 cgcgcgggcg cgggcggcag gcaaacccga cgccggtggc cgctactgcc cgggagttgg
      481 gcctccccct gctgcagccg gccaaagtca atgacccaga ggtcgtggag caggtgcagg
      541 agttggcgcc ggacgcgatt gtcgtcgctg ctttcggcca gatcctgcgc aagccgcttc
      601 tgcagattcc cgctatcggc tgcgtcaaca ttcacgcctc gttgctgccc cagtaccgcg
      661 gtgggtcacc tgtgcagcat gcgctcctcg ctggcgagcg cgtcaccggc atcaccatca
      721 tgctcatgga tgaggggatg gataccggtc ccatcctgat gcagcgggaa gtgctgattt
      781 cgcccgagga tacagcggga acgctgctcn nnnnnnnnng accggctcgc gcgtgtcggc
      841 gctgacctgc ttctggaggc gcttccggcg ctggcggcgg ggcgcctgga gccgaccccc
      901 caggatgcct cccgggccac atatgctccg aacctgtcgc gtgaggacgc gcgcatcgat
      961 tggagcagca gcgccgagaa gatccgcaat caggtgcgcg cgttcagtcc gaaaccgggc
     1021 gcctgggccg agttccgcgg caaggaagtg aagatctggc gcgcgcgcgt cgattctgat
     1081 gccacggatg cgtcgcctgg gcagatcgta gccattgggc cggagagtat ccgggtcgcc
     1141 accggcgagg gtaccctctg gctggaggag gttcaagagg caggcaagag ccggatggga
     1201 gctggcgcct ttgcccgcgg cgcgcgcctt gcccccggcg aacgcttctc ctgaatcaag
     1261 atgtgggcac gcggcccgca tcgccgctgg cccgcggaag atgcgcgctt ctcctagcgg
     1321 ttggactcct tgatcgtcgc gaggagctca tccatgcgcg ccttaccggc ttccgcctcc
     1381 tggagttgct gccgtagcga ttcgatgcgt tccgagagct ccgaaggagt ctcagcgatg
     1441 atgcgtgcct gaagctcctc cgcggcccgg cgcgccgcga gagctttttc catgacggca
     1501 tccgcctgca tccgatagcg catccagcgg aggatgggaa tgaacgccca cccgccaagc
     1561 atgacggccg acagcagcag tccgacgggc gtgcggagga cggccgcgat ctcgccgtgg
     1621 agagcagcca ggccgcccag gaaggcggcc ccggccagca taatcccgca tccggcaagg
     1681 gcatgttcct ccagtggagg cggtgcttcc aggagggctt tgcactcgcg ctccgctgcc
     1741 tctgcctcag cttccttctg ccgcacctcg ggcgagcttt cccagcccgc ctcgtggatg
     1801 gcattttcca gctcttcttt gagtgccgcc gtggcgtctc tgcgcctgca ggcctcttcc
     1861 tctagctcgc gcgtggcgaa ggcgagcgca ggcgccgacg cgcgcgccgc gtagcgctcg
     1921 atctcaagca ctgtcaggta gccggcatcg gtcacctccg atgcggcggc atcagcgcat
     1981 gatcgcccta gataggtcgg aagctcaacg gtatgcccgg actgctcttg ccaggaggcg
     2041 atgcggtcga gagcgtcgct ccaatgcgtg accatcgtgc gcgcctgata gcgggcctga
     2101 gagaggactc gcgatatcag ttcgtgcagc tcgcgggcta gcgccgtgaa gtccgcctcg
     2161 gcgagcgcgg agatggcgac cgcgggagag cgttggatgc attgcgcgat ccactcttga
     2221 gcctcggaga tgcgtccggt gagcgccgca tagcgggcgc catcaaagag gacctctggg
     2281 tcgagcgaga cctgaagggc atcggcgatg gcccgatagg caccgacgat gttgccacgc
     2341 aagtagcgga tgtgagcgac gtgccggagg ctctccacgt agaacaggtc gcgggtgacc
     2401 gttgtcagcc gggcgcactg tgcaaaggcc tcctccgcct cgtgcagccg ctgggcgtgc
     2461 ttccaggcga gccaaccaag ctggaaccag gccgcgtgat cgcgccggcc cgcacggtcg
     2521 tctacgacac gtcgaagtag cgagagggcg ttctgatggt aggcgcgacg ctcggctcct
     2581 gtgctccgta acccgttgcg caatgcctca tccgcgcgcc aggagcgttc ctgaagctcg
     2641 cgggggaacg tctggtgtgt ggcgccggtg agactctcga gcatccgctg cgccgtctgg
     2701 atccgctccg ccgcc
//
LOCUS       DAOSCV010000624         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24305_length_2713_cov_3.624906, whole genome shotgun sequence.
ACCESSION   DAOSCV010000624 DAOSCV010000000
VERSION     DAOSCV010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24305_length_2713_cov_3.624906"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLY56_14135"
     CDS             <1..195
                     /locus_tag="PLY56_14135"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009590099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ22665.1"
                     /translation="TDCNGATAGSVLGTLLGANALPEAWVAPLNDRLHSIVTGFSETR
                     LSELAQRTCAQVEKVASVQG"
     gene            520..1395
                     /gene="motA"
                     /locus_tag="PLY56_14140"
     CDS             520..1395
                     /gene="motA"
                     /locus_tag="PLY56_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOJ22666.1"
                     /translation="MLLIVGLVVVFGSIVVGYTMHGGRIAALVQISEFIIIGGAGLGS
                     VLVANPPSVVAKTFREIFGLVRGNPYTKARYMELLQMLYDMFMMARREGVVALDQHVE
                     RPEESSFFRRYPFFHSNHHALSFLADTMKVMISGSVATYDLMELMDVDLETMREEAMR
                     PSHIMAKVADAMPGFGIVAAVLGVVITMGAIGGPPEEVGHKVAAALVGTFLGILFSYG
                     IFGPIAAAMEAQVNSEVQYLSCIKYALLSFGRGDAPLTGVEFARRNIEPSVRPTFTEM
                     EETLRSGARATARAA"
     gene            1464..2267
                     /locus_tag="PLY56_14145"
     CDS             1464..2267
                     /locus_tag="PLY56_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ22667.1"
                     /translation="MAEGMGKDNPPIIRIVRKKQVVRHHSGAWKVAYADFVTALMAFF
                     LVMWIVGLSKPVREAIEAYFKDPIGFSRAVRHGRSPFATGDAMGILTGGAAPIPAGKG
                     GGVKSKEEQLREVQQAIVEEMERLPELKSLRDSVSVTLTREGLRIELIEKRSSLFFDS
                     GSAKLKPHTRHLLGVIAKQLRDVNNPIVIEGHTDSLPLVSEPHYSNWELSADRANAAR
                     RAMEASGLRPRQVLAVRGYADRKPLRPRDPRHYSNRRVSILVAYSTAGH"
     gene            complement(2298..>2713)
                     /locus_tag="PLY56_14150"
     CDS             complement(2298..>2713)
                     /locus_tag="PLY56_14150"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HOJ22668.1"
                     /translation="AMNELDRAEEFHGHLGPWLVVGMRVGQAALERVDARKYFGVRVE
                     VAVPPAPPVSCLVDGLQLGTGATTGKANLVVSRADPPRVRVINTETGRSATITFAEGL
                     SAKLAAWLKEEGDRAAARRVLEAPLEELCAIVPEP"
BASE COUNT          468 a          908 c          892 g          445 t
ORIGIN      
        1 accgactgca acggcgcgac ggcgggctcc gtcctcggaa cccttctggg cgccaacgca
       61 cttcccgagg cctgggtcgc tcccctcaac gaccgtctcc actcaatcgt caccggcttc
      121 tcggagacgc gtctctcgga actcgcccag cggacgtgcg cgcaggtcga gaaggtggca
      181 agcgtccagg gataggacgc ggaatcgagc cccgcgccgc cggcgctcgc cggcggcgct
      241 ctgaatgacc gtttcacggg tcgctttgtc ccccagcacc gtgggcctgc tcctcgcgtg
      301 tgcgaattgg atccacgaac ggcctcgctc gcgcggttgt ccaagagccg tgcctctccg
      361 acaggcttaa ggatggcccg ccgggagccg aaaccccaag tagagctatc atccaccagg
      421 agccgggagc gatccgcgcg tgcgtgccca tggctgaggc gcgccagcat gagcccttca
      481 tgccccggcc gacgccagtc tgggaagcga gaaggtacta tgctgttgat cgtggggctt
      541 gttgtcgtct tcgggagcat cgtggtgggc tatacgatgc atggcggcag gatcgcggca
      601 ctggtccaga tcagcgagtt catcatcatc ggcggggcag gcctcgggag cgtgctcgtt
      661 gccaacccgc cgagtgttgt cgccaagacg ttcagggaga tctttggcct ggtgagggga
      721 aacccctata ccaaggcgcg ctacatggag ctgctccaga tgctctacga catgttcatg
      781 atggcgcgcc gggaaggggt ggtcgctctc gaccagcatg tagagcgccc ggaggagagc
      841 tccttcttcc gcagataccc cttcttccat agcaaccacc acgcactctc cttcctggcc
      901 gacacgatga aggtgatgat cagcggttcg gtggccacct acgacctgat ggagctgatg
      961 gacgtggacc tcgagacgat gcgcgaggag gcgatgcggc cgtcgcacat catggcgaag
     1021 gtggcggatg ccatgcccgg ctttgggatt gtggcagcgg ttctcggcgt cgtgatcacg
     1081 atgggcgcga ttggcgggcc tccggaggag gtcggccaca aagtggcggc cgccctggtc
     1141 ggcaccttcc tgggcatcct gttctcctac ggcatcttcg ggccgatcgc tgccgcgatg
     1201 gaggcgcagg tcaactcaga ggtacagtac ctgtcatgca tcaagtatgc gctcctctcc
     1261 ttcgggcgcg gcgatgcgcc actcacgggc gtcgaattcg cccggcgcaa catcgagccg
     1321 agtgtgcgtc ctaccttcac cgagatggag gagacgctga ggagcggtgc acgggcaact
     1381 gcccgcgccg cataaacgag agcgctgctt gagggcgatg gaacagccgg tcgcccacac
     1441 accgcattgg gaggagaaac cgcgtggccg aaggcatggg gaaggacaac ccgccgatca
     1501 tccgcatcgt gcgcaagaag caggtggtac ggcaccacag cggcgcctgg aaggtcgcct
     1561 atgctgactt cgtcacggcg ctgatggcgt tcttccttgt catgtggatc gtggggctga
     1621 gcaagccggt tcgcgaggcg attgaggcct acttcaaaga ccctattggc ttcagcaggg
     1681 cggtgcgcca cggccggagc ccgttcgcca ctggcgatgc catgggcatc ctcaccggag
     1741 gcgcggctcc cattcctgcc ggcaagggcg gcggcgtcaa gtcgaaggag gagcagcttc
     1801 gcgaggtgca gcaggcgatc gtcgaggaga tggagcggct gccggagctc aagtcgctgc
     1861 gcgacagcgt ctctgtcacg ctcacccggg aaggcctgcg cattgagctg attgagaagc
     1921 ggtcctcgct cttcttcgac agcgggagcg caaagctcaa gccgcacacg cgccacctgc
     1981 tcggcgtgat cgcgaagcag ctgcgcgacg tgaacaaccc gatcgtgatc gaggggcaca
     2041 ccgactctct cccgctggtc agcgagccac actactccaa ctgggagctc tccgccgacc
     2101 gggccaacgc ggcacgccgg gcgatggagg ccagcggcct gcgccccagg caagtcctcg
     2161 cggtgcgcgg ctatgccgac cggaaaccgc tgcgcccaag agacccccgg cactactcca
     2221 accgccgggt gagcatcctg gtcgcctact ccaccgcggg ccactaagcg cgccggcccc
     2281 caggcgagcg caccgcgtca gggctcgggc acgatcgcgc agagctcctc cagcggcgct
     2341 tccaacacgc gccgggcggc agcccggtcg ccctcttcct tcaaccaggc tgccagtttg
     2401 gcggaaagcc cctcggcgaa ggtgatcgtg gcgctccggc cggtttcggt attgatcacg
     2461 cgcacgcggg gcggatcggc gcggctcacc accagattcg ccttgcctgt ggtcgcgccc
     2521 gtgcccagtt gaagcccatc caccaggcac gagacggggg gcgcgggcgg cacggccacc
     2581 tccacgcgca ctccgaagta cttccgcgca tccacgcgtt cgagtgccgc ctggcccacg
     2641 cgcatgccga caaccagcca cggccccagg tgcccgtgga actcctctgc ccggtctagt
     2701 tcattcatcg cac
//
LOCUS       DAOSCV010000625         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24321_length_2712_cov_3.294317, whole genome shotgun sequence.
ACCESSION   DAOSCV010000625 DAOSCV010000000
VERSION     DAOSCV010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24321_length_2712_cov_3.294317"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /gene="argB"
                     /locus_tag="PLY56_14155"
     CDS             <1..558
                     /gene="argB"
                     /locus_tag="PLY56_14155"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ22669.1"
                     /translation="VLAGKTNKGIVSLINRKGGKAVGLSGKDANLIRARKRPGPDLGF
                     VGEVTEIDSSIIDVLVEKGFIPVICSVAIGEAGESYNVNADHVAGQLAAALGAVKLIM
                     LTDVEGIYEDFSDKSTLQSQLSPEKARAMIASGQIEKGMIPKVEACLMALEGGVERTH
                     IIDGRLSHATLIELFTDRGIGTMIA"
     gene            complement(738..959)
                     /locus_tag="PLY56_14160"
     CDS             complement(738..959)
                     /locus_tag="PLY56_14160"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ22670.1"
                     /translation="MSPSGDALVRQRVYDAIDAEEMLRDASIEASVTDGVALLRGEVA
                     TETQRSRIEAVVSQVEGVTGIQNEIRIRQ"
     gene            1157..2440
                     /locus_tag="PLY56_14165"
     CDS             1157..2440
                     /locus_tag="PLY56_14165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22671.1"
                     /translation="MARIAETEGQRRTTLFLCAVLHAFTHLYPTVLPPLYYQVARDLE
                     LSGVWLATLLVSAQSLAYCLAGWPLGFLADRVSRKWLMFWGLAINGAAFVALGLAPSY
                     TVALLCLVIAGLAGGAYHPPATALLTGLYPERQGRAIGLAGMGAGVGFFLGPFYGGLR
                     GEVAGWRAPCLELGLAGLAVAILFALLARDAPAAKKQAVPAAGPAFVSYFILLSVGLG
                     LRDFAGTGFTTFAALFLPQAHGFSQRTTGLYLGLMSLSAVAANPFFGAASDGRYRLWW
                     AGGVLLLAGLGGLALPWLPKPLVLLGLLWWGFFLLGSYPITEAAMSEVLPDQIRGRAF
                     GVFLTSSGLLSSAAPWVMGTIKDRAIGASTDPAAFRPAFAGLALLMLLSVLIGMPLLR
                     WVRRELARMTARDGCAPDQGCAPLAAAGSKRPPAS"
     gene            2460..>2712
                     /locus_tag="PLY56_14170"
     CDS             2460..>2712
                     /locus_tag="PLY56_14170"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ22672.1"
                     /translation="MDERSELHAPARARGRLRVLYTAEEIRARTARLADEIRPYYEGK
                     NPLLLGVLKGSFIFLADLARALAIPLEVDFVQLASYGDGT"
BASE COUNT          382 a          914 c          940 g          476 t
ORIGIN      
        1 gtcctggcgg gcaagacgaa caaggggatc gtctcgctga tcaaccgcaa aggcggcaag
       61 gcggttgggc tctcggggaa ggacgccaat ctgatccgcg cacgcaagcg ccccgggccg
      121 gacctgggct tcgtgggcga ggttaccgag atcgactcct cgatcatcga cgtgctggtg
      181 gagaagggct tcatcccggt gatctgctcg gtggcgattg gcgaggcggg cgagtcttac
      241 aacgtcaacg cggaccacgt ggcgggccag ctcgctgccg cgctcggcgc cgtgaagctg
      301 atcatgctca cggatgtcga ggggatctac gaggatttca gcgacaagag cacgctccag
      361 tcgcagctca gcccggagaa ggcgcgggcg atgatcgcca gcgggcagat tgagaagggg
      421 atgatcccga aggtggaggc gtgcctgatg gcgctggagg gtggcgtcga gcggacgcac
      481 atcatcgatg gccggctgtc gcacgccacg ctcatcgagc tgttcacgga tcgcggcatc
      541 ggcacaatga tcgcctgagg tatcccctgg gcgaagggag acgcgcgccg gcgcgccggg
      601 tgcgcgcggc gcgcggcggt ccatagcgga ggcaaccgag atggaagaac cggaaggcgg
      661 accagggatc agccctgtcg ccgtcacgat cctggcgatc gtgctgttgc tgtcgctctg
      721 gctgctctac cgccacctca ctgcctgatc cggatctcgt tctggatgcc ggtgacgccc
      781 tccacctggc tgacgacggc ttcgatccgg ctgcgctgcg tctccgtggc aacctccccg
      841 cgcaggagcg cgacgccgtc ggtcaccgag gcctcgatgg aggcatcccg gagcatctct
      901 tcggcatcaa tcgcatcgta aacccgctgg cgaacgaggg cgtcccccga tgggctcaag
      961 ggatcctgct cgtcgtgaga acgtatgtca caaccgaccc tcatggcagt tcctcccctc
     1021 tgtctgccgg agcttacccc ggggcgtgga atcgccaaac gcgccgccat cctcggggtt
     1081 tcgcgcggag ggcggaaccg ccaggagccc cgggcgctga gaggggccgg gggcattccc
     1141 ccgcgcgagg agattcatgg cacggatagc ggagactgag gggcagcggc gcacgacgct
     1201 tttcctgtgc gccgtgctgc acgccttcac ccatctctac ccgacggttt tgcccccgct
     1261 ctactaccag gtcgcgcggg atctggagct ttcaggcgtg tggctcgcca cgctcctcgt
     1321 ctccgcgcaa tcgctcgcct actgcctggc gggatggccg ctgggcttcc tggcagaccg
     1381 cgtcagccgc aagtggctga tgttctgggg cctggcgatc aacggtgcgg cgttcgtggc
     1441 ccttggcctg gccccatcgt acaccgtggc gctcctttgc ctggtcatcg ccggactagc
     1501 cggcggcgcc taccacccgc cggcaaccgc gctcctcacc gggctctacc cggagcggca
     1561 ggggcgggcg attggcctgg ccgggatggg cgccggcgtt ggtttcttcc tcgggccgtt
     1621 ctacggtggg ctccgcggcg aggtggccgg ttggcgcgcg ccctgcctgg agcttggcct
     1681 ggcggggctc gcagtggcca ttctcttcgc gctccttgct cgggatgccc ccgcggcgaa
     1741 gaagcaggcg gtgccggctg cgggcccggc gttcgtgagc tatttcatcc tgctgagcgt
     1801 gggcctcggg ttgcgcgatt tcgcgggcac cgggttcacc accttcgcgg cgctcttcct
     1861 gccccaggcg cacggcttca gccagaggac caccgggctc tacctcgggc tgatgagtct
     1921 ctctgccgtg gcggcaaacc ccttcttcgg cgccgcgtcc gatgggcgct accgcctctg
     1981 gtgggcgggg ggcgtcctcc tgctcgccgg actgggtggc ctggctctcc cgtggctgcc
     2041 aaagccgctc gtgctcctcg gtctcctgtg gtggggattc ttcctgctgg gctcctaccc
     2101 gattactgag gccgcgatga gcgaggtgct tcccgatcag atccgcgggc gcgcgtttgg
     2161 tgtctttctc accagcagcg gattgctcag cagcgccgcg ccctgggtga tggggacgat
     2221 caaggaccgg gccatcggcg cgtccacaga cccggcggca tttcgcccgg ctttcgcggg
     2281 tctcgcgctc ctcatgctcc tctccgtgtt gattggcatg ccgctactgc gctgggtgcg
     2341 ccgcgagctg gcgcgcatga cggcgcggga tggatgcgcg cccgaccagg gatgcgcgcc
     2401 actcgccgcc gccggatcca agaggccccc ggcctcctga cgaatgcgag gaaggcagaa
     2461 tggacgagag aagcgagctc cacgccccgg cacgcgcgag gggccgcctg cgcgtgctct
     2521 acaccgcgga ggagatccgc gcgcgaacag cgcgcctcgc cgatgagatc cggccatact
     2581 atgaaggcaa gaatcctctg ctcctgggcg tgctgaaggg ctccttcatc ttcctcgccg
     2641 acctggcgcg cgccctggcg atccccctag aggtggactt cgtccagctc gctagctacg
     2701 gcgacggcac cg
//
LOCUS       DAOSCV010000626         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24378_length_2708_cov_3.496042, whole genome shotgun sequence.
ACCESSION   DAOSCV010000626 DAOSCV010000000
VERSION     DAOSCV010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24378_length_2708_cov_3.496042"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..509)
                     /locus_tag="PLY56_14175"
     CDS             complement(204..509)
                     /locus_tag="PLY56_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22673.1"
                     /translation="MDIYSEDGPNDLEGAGPGGGADIVREHEAEPIPGSRLGLVVTVA
                     LVALVLLVPVLWAAGVYAGLVILPGVGPAATAGYLLLVILIAAGAALYFWAQFRKSG"
     gene            867..1439
                     /locus_tag="PLY56_14180"
     CDS             867..1439
                     /locus_tag="PLY56_14180"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HOJ22674.1"
                     /translation="MANGKRQGPVATGRSDRASANLPNVRRRGTVPIRRNVEDFEDPY
                     IPEESYAGTVICRTCHAVHQNERWSLDPKIYEEAVKTEGVQYITCPACQKLRDRQPGG
                     IVTLSSDFIPEHRQEILNLIRNEDERARNVNPLERVMEIEEDTNEIVVTTTNEKLAQR
                     LGRALYRAYKGHVEYHWMGTSKQARVRWTR"
     gene            1930..>2708
                     /locus_tag="PLY56_14185"
     CDS             1930..>2708
                     /locus_tag="PLY56_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOJ22675.1"
                     /translation="MRLFARRRRHLARQYQVAVTIARNMGPAAIDALGLRHLVPRRLR
                     QPVSVPDPASKLRATMEELGPTFVKLGQLLSTRPDLITDPRYLAELSKLQDTAPAIPF
                     EKVRAVIEEELHRPLSEVFEFFSERPLAAASLGQAHTAILKGGTAVVVKVQRPGIGRV
                     IETDMEIIRNSANFVMQRFELARVLNIVDLVEEFHLTMREELDYTREGRNMDRIRENL
                     RTERQICVPRVYWDVTTSRVLTQERIRGVKITDVAAIRDAG"
BASE COUNT          505 a          931 c          859 g          413 t
ORIGIN      
        1 ccatccacac tcggaggcgg atcgggacag cgcggggcga cgaggacggc gtcttgggcc
       61 aacaccgggc cggtgctgag caggagaacg aggaaggcaa gcaggcgcag cattcgggat
      121 ctccctccat cgcggcgggg ggcgctctcg cgccggatcg ctcccgccgt cataggggag
      181 attcgccgct gcctgccggc atcctacccg ctcttgcgaa actgcgccca gaagtagagc
      241 gcggcgcctg ccgcgatgag gatcaccagg aggaggtagc ccgcggttgc cgccgggcca
      301 actcccggca agatcaccag gccagcgtac accccagccg cccagagcac gggaaccagc
      361 agcacgagcg cgacgagcgc taccgtcacc accagaccca gccggcttcc gggaatcggc
      421 tcggcctcgt gctcgcggac gatatccgcg ccgccaccgg gccctgcgcc ctccagatcg
      481 ttgggaccat cctctgaata gatatccatc gcccggctcc tctcccacga gcgccttcgc
      541 gcccgtgtcc gtttcggttt ccccgggcaa gagcaccgcc aaacgccgcc agcccgcccg
      601 aggcgtggca gggcccaccc gggtttgagc agcttgcaac gacattcgcc ggccatggcc
      661 acttgcctct ttcagatcca gaaaaagcga gcaggctacg caccgtgccc actcacccgg
      721 ctgtcctcta agcgcatgag gcatgccgct tgccgtttga ggcgagggta ggcgtggaag
      781 aatcccaatg gaggagcgcc tgccccgctc ggtggccggc gaatctgatc ggataaccgt
      841 ggatcgcaca gagaggaagt gacgaagtgg cgaacggcaa gcgacaaggc cctgtggcca
      901 cgggacgcag tgatagggct tcggccaacc tccccaacgt gagacggcgc ggtaccgtac
      961 ccatccggcg caacgtggag gatttcgaag acccgtacat ccccgaggag agctacgccg
     1021 gaacggtgat ctgccgcacc tgccacgccg tgcatcagaa cgagcggtgg agcctggatc
     1081 cgaagatcta tgaggaggcg gtgaagaccg agggggttca gtacatcacc tgccccgcct
     1141 gccagaagct ccgcgaccgg caaccgggag gcatcgtgac cctctccagc gacttcatcc
     1201 cggagcaccg ccaggagatc ctcaacctga tccgcaacga ggacgagcga gcccggaacg
     1261 tcaacccctt ggagcgggtc atggagattg aggaggatac caacgagatc gtggtgacga
     1321 ccaccaacga gaagttggcg cagcgcctgg gtcgcgccct ctatcgggcc tataagggcc
     1381 acgtcgagta tcactggatg ggcacttcca agcaggcacg cgttcgctgg actcgctagc
     1441 tggcccctca gtggggtcag gggcgtcggc cagcgggcaa tcggggcgga gtgacgcccg
     1501 ctggaagaag cttccattcc gccggcccgc gcccgggcga gtggccatgc ctcgacccca
     1561 aggggtcgga caacatagcc tagggtgtcc gcaccctggg gcatgcgccc gcaaaataac
     1621 cacgatctcg aagaggtcgc acaaaccgcc accgcgtctt gtcgaacccg ttccgggttc
     1681 caggtatttt ctggctgcga gacccagggt caggagaccc tgggctatcc tgtgggcccg
     1741 ctccgcggtc ccccgagagc gccacccggg tgtcgcccgc agatcccggg cacgcgcgtc
     1801 cggcccatcc gtgtcccttc ctcagtggca tcgacgccgg cgcgcgcccg cggcaacgct
     1861 agatgaaggc gatcccgcgc gcaacgccga acgattccat agtatctccc tggagggtaa
     1921 cacaagagga tgcgtctctt cgcccgccgg cgccggcacc tggcacgcca gtaccaggtc
     1981 gccgtcacca tcgccaggaa tatgggtcct gcggctattg atgccctcgg gctgcgccac
     2041 ctcgtccctc gccgcctgcg ccagcccgtt tccgtgccgg atccggcatc caagctgcgt
     2101 gccaccatgg aagagctggg ccccaccttc gtgaagcttg gccagctcct gagcacacgc
     2161 ccggatctga tcacggatcc gcggtacctc gcggagttga gcaagctgca agacaccgcg
     2221 cctgctatcc ccttcgagaa ggtgcgcgcc gtcatcgagg aggagctgca ccgcccgctg
     2281 tcggaggtct tcgagttctt ctccgagcgc cccctcgccg ctgcgtcgct cgggcaggca
     2341 cacaccgcca tcctcaaagg tggcacggcc gtcgtagtga aggtccagcg tcctggcatt
     2401 ggccgggtca tcgagaccga catggagatc atccgcaact ccgccaactt cgtcatgcaa
     2461 cgcttcgagc tggcgcgggt gctcaatatc gtcgacctcg tcgaggagtt ccacctcacc
     2521 atgcgcgagg agctggacta cacgcgcgaa ggccggaata tggaccggat tcgcgagaat
     2581 ctgcgcaccg agcgtcagat ctgcgtgccg cgagtttact gggacgtgac gacctctcgt
     2641 gtgctcacgc aggagcggat ccgcggcgtt aagatcaccg atgtggcggc tatccgcgac
     2701 gcggggat
//
LOCUS       DAOSCV010000627         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24453_length_2702_cov_3.089913, whole genome shotgun sequence.
ACCESSION   DAOSCV010000627 DAOSCV010000000
VERSION     DAOSCV010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24453_length_2702_cov_3.089913"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(164..877)
                     /locus_tag="PLY56_14190"
     CDS             complement(164..877)
                     /locus_tag="PLY56_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22676.1"
                     /translation="MMTPRQRYCETLLFGTPDRIPFQPGSPRVSTLAVWRQQGLPEGV
                     YWYDYLLECLGIAPDHGRGGGDPGVSFRMIPTFEEKVLEHRDGHYIVQDWMGAITEIS
                     DRYDYTYIRSAKDFVTRKWHRFPVQTRDDWERMKERYDPRAPGRFPADFEERCARMKE
                     RTGVAWIYFNGPFWQMREWCGLEGLCVLMADDPEWVEEMATFWMEFVSQTMAPILERV
                     ELDRVCISEDMAYKANSMI"
     assembly_gap    1005..1014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1015..1660)
                     /locus_tag="PLY56_14195"
     CDS             complement(<1015..1660)
                     /locus_tag="PLY56_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22677.1"
                     /translation="MKRSAGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCQS
                     NLKQLGMAAMQYAQDYDETYPMMYRRMPHLYWWSDVLQPYVKNYQVLVCPSGSWSTAT
                     ERPPSLPNPLVCSYAFPYFSKTDAGAAITPLPGAPMASVPDPAGTIMFCDSTTTEIYS
                     GGVNNFTIAGPNGDTDLGTGNYVRVARRHMDGFNCAFADGHVKWLRRSTPGMWTT"
     gene            complement(1783..2445)
                     /locus_tag="PLY56_14200"
     CDS             complement(1783..2445)
                     /locus_tag="PLY56_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22678.1"
                     /translation="MRRSAGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCQS
                     NLKQLGMAAMQYAQDYDEIYPAVYRRMPDLYWWGDMLQPYIKNYQVLVCPSGSWSTPN
                     ERPPSLPNPLVCSYALPNIRKNDAGADITPLPGASMAQVSDPAGTIMLCDSSRSEIFT
                     PEATYNFTIAGPNGVTDLGTGSYVRVALRHMDGFNCAFADGHVKWLRQSTPGMWTTLA
                     GD"
BASE COUNT          502 a          845 c          853 g          492 t
ORIGIN      
        1 cttcgacggg gcagcaggcg ttgatccccg cttcgatcca gagaggaatc aactcgccaa
       61 tgtagccgtc ggagtccatg tcgatgatcg ggcagccgct cttcttgatc tcggggatcc
      121 atcgctcgta gcagggcatg aggaagcggc ggatcatggc gggctagatc atgctgttgg
      181 ccttgtaggc catatcctcc gagatgcaaa cccggtcgag ctccacgcgc tccaggatgg
      241 gggccatcgt ctgcgagacg aactccatcc agaaggtggc catctcttcc acccactccg
      301 ggtcgtcggc catcagcacg cacaggccct ccaacccaca ccactcgcgc atctgccaga
      361 aggggccgtt gaagtagatc caggcgacgc ccgtgcgctc cttcatgcgc gcgcaccgct
      421 cctcgaagtc ggccgggaag cggccaggtg cgcgcgggtc gtagcgctcc ttcatgcgct
      481 cccagtcatc gcgcgtttga acagggaagc ggtgccactt gcgcgtgacg aagtccttgg
      541 cagagcggat gtaggtgtag tcataccggt cggagatttc ggtgatcgcc cccatccagt
      601 cctggacgat gtagtggccg tcgcgatgct cgagcacctt ctcctcgaag gtggggatca
      661 tgcggaagga gacgcccggg tcgcccccgc cacgcccatg atccggggcg atgccgaggc
      721 actccagcag gtagtcatac cagtaaacac cctcggggag gccttgctgg cgccacacgg
      781 cgagagtcga cacccgcggg ctgccgggct ggaagggaat gcggtccggg gttccaaaga
      841 gaagcgtctc gcagtagcgc tggcgtggtg tcatcataga ttccaattct catggggcaa
      901 ggagcggggg gaggcagcat cgccacggcg tcctccccgc cctttcccat tcggcagcgc
      961 ccgcgcggct tgcagccgag cgcctgtcag tgtgagcacg caggnnnnnn nnnngagtcg
     1021 tccacatgcc gggcgtgctc cgcctcagcc acttgacgtg cccatcggcg aatgcgcagt
     1081 tgaaaccgtc catatgccgc ctggcgacac gaacgtagtt gcccgtgccc agatcggtgt
     1141 caccgttcgg acccgcgatg gtgaagttgt tcacgcctcc gctgtagatc tcggtagtcg
     1201 tggagtcgca gaacatgatc gtgcccgcgg ggtccggaac ggaggccatc ggcgcaccgg
     1261 ggaggggcgt gatggctgcg cccgcatcgg tttttgagaa gtacgggaaa gcataggagc
     1321 agacgagcgg gttgggaagg ctcgggggcc gctcggtggc agtgctccag gagccggacg
     1381 ggcagaccaa cacctggtag ttcttgacgt agggctgcag cacgtcgctc caccagtaca
     1441 gatgcggcat gcggcggtac atcatcggat aggtctcgtc gtagtcctgc gcgtactgca
     1501 tcgccgccat gcccaactgc ttcaggttgc tctggcaggt ggacttgcgc gcgttctcgc
     1561 gtgccctggc aaagaccggg aaaaggatcg ccgccaggat ggctatgatg gcgatgacca
     1621 cgagcagctc gataagcgta aaaccggcag aacgtttcat cggtcctttc ctcctaactc
     1681 ctgccgcgga tggtcacact agaagacgcc cgtggggagg ccttgccggc gccatgcggc
     1741 cagagccgcc tcccccaggc tgctggagtg ccacgcgggc cactagtcgc cggcgagagt
     1801 cgtccacatg cccggcgtgc tctgcctcaa ccacttgacg tgtccatcgg cgaacgcgca
     1861 gttaaaccca tccatatgcc gcagggcgac acgaacgtag ctgcccgtgc ccaggtcagt
     1921 gactccgttt gggcccgcga tcgtgaaatt gtaggtcgcc tccggggtga agatctcaga
     1981 tcgcgacgag tcgcacaaca taatcgtgcc cgcggggtcc gaaacctgcg ccatcgacgc
     2041 gccagggagg ggcgtgatgt ctgcgcccgc gtcgtttttc cggatgttcg gaagagcata
     2101 ggaacagacg agcgggttgg gaaggctcgg tggccgctcg ttgggagtgc tccaggagcc
     2161 agacgggcag accagcacct ggtagttctt gatgtagggc tgcagcatgt cgccccacca
     2221 gtacaggtcc ggcatccggc gatacaccgc cggatagatc tcgtcataat cctgcgcata
     2281 ctgcattgcc gccatgccca actgcttcag gttcgattgg caggtggact tgcgcgcatt
     2341 ctcccgcgcc ctggcaaaga ccgggaaaag gatcgccgct agaatggcta tgatggcgat
     2401 gaccacgagc agctcgatga gcgtaaaccc ggcagatcgt ctcatggatg ccttcctcct
     2461 actcctacgg gtacacggaa tcataaccca cgctttccat ccatcccttg ctcgctctgt
     2521 tccccacccg ccatgctatc tcctgcgcga ttaccgcccc gcgggtgaac tgcaaccatg
     2581 cgtgcgatag tgcgagcgga ggcttgccac gctcgccgaa aaagcgtgcg cgttgaggat
     2641 gtgggctgac tctatcggga acggctcctc ctaccagttc accatcctgc aggagaacag
     2701 cc
//
LOCUS       DAOSCV010000628         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24563_length_2693_cov_3.253222, whole genome shotgun sequence.
ACCESSION   DAOSCV010000628 DAOSCV010000000
VERSION     DAOSCV010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24563_length_2693_cov_3.253222"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2693)
                     /gene="smc"
                     /locus_tag="PLY56_14205"
     CDS             complement(<1..>2693)
                     /gene="smc"
                     /locus_tag="PLY56_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOJ22679.1"
                     /translation="EARAAASAASQKVERARGTLGVSRERLASVEAGEADDAERIADL
                     ERALERCRREKEEASERIAAAEAAERAATEELRRCEAEVAAIQGRMEKLARVVEEHRR
                     QAVERARALTNAQADLRASGRQVQDARAALTRATQRLEMLGARAATQQAREEGLREKV
                     TEAERRCEQVNEQLRRAEAERATLQRGIVLAGQRMTELRTEITARSTRLRTLRELDAK
                     LEGYRDGARAILRARKEGRLKGHFVPIVDLLEVSPEFEAAIHAALGDDLDALVVDSLE
                     EAYEAITYLRKEGKGRATFVVREAAVPVADGTEAQEILSRRATQHVRFADAHRNVIGA
                     VLGNAVVVRHLEEAREVLALDHARYRAVTLEGDLLHPRGILTGGRANGGAASLLARRR
                     EMAGLEEETARLENELARVTKENETKSAALRAAEVSLRRQREEASRAASALAAAKKDL
                     DYCAQEARRLADDLRRAEQSIHQAEADVEKAVAREARLRAHVEQLQTQDSGADADIES
                     ARASLEELNRERDRAAEAVADQKVAAARARQQVEGQRENAARAHRQEEETLRDIERRR
                     AHMASRAERVRALREEIAGQEAELAQFVAAHELAQKELDERRAERRRLLEASGKIEQQ
                     AKEVRDRLAAAQHELHRAEVKLAGVEADIQHAQTSLYEQYGLTADALQQVSREGVPEI
                     RNRQQAGQELRQLRAQMAELGEVNLGAVEEVERLEERLRFLTTQREDLTQAREGLLEV
                     IAEIDSETRGRFMRTIEQLRQAFNEIFVRLFGGGTTAIRLTDESDVLEGGLEIVVQPP
                     GKKPQSMLQLSGGERALTATAFLFALLRIKPSPFVLLDEVDAPLDELNVARYAAMLKE
                     FAEKSQFIVITHNKGTMEAADVLYGVTMPEQGVSRIVSVRL"
BASE COUNT          369 a         1016 c          831 g          477 t
ORIGIN      
        1 caggcgaacc gagacgatac gcgagacgcc ctgctccggc atggtcacgc catagaggac
       61 atcggccgcc tccatcgtcc ccttgttgtg cgtgatgacg atgaactgcg acttctctgc
      121 gaactccttc agcatggcgg cgtaacgcgc gacgttcaac tcgtccagag gcgcatccac
      181 ctcatcgagg aggacgaagg ggctcggctt gatccgcagc agcgcgaaga ggaaggcggt
      241 cgctgtcaga gcccgttccc cgccggagag ttggagcatg ctctgcggct tcttcccggg
      301 gggctgcacc acaatctcaa ggccgccttc caacacatcg gattcatccg tgagccggat
      361 ggccgtcgtc cccccgccga agaggcgcac gaagatctcg ttgaacgcct ggcgaagctg
      421 ctcgatggtg cgcatgaagc gcccccgggt ctccgaatcg atctcggcga tcacttcgag
      481 gaggccctcg cgcgcctgcg tcagatcctc gcgctgggtc gtcaggaacc tcagccgttc
      541 ctccaggcgc tccacttcct cgacggcgcc caggttgacc tctcccaact ccgccatctg
      601 cgcccggagc tggcgcagct cttggccggc ctgctggcga tttcggatct ccgggacgcc
      661 ctcacggctc acctgctgaa gcgcgtcggc cgtcaatcca tactgctcgt agagagaggt
      721 ctgcgcgtgc tggatatccg cctcgacacc ggcgagcttc acctctgcgc ggtggagctc
      781 gtgttgcgcg gcggccaggc ggtcgcgcac ctctttggcc tgctgctcta tcttgcccga
      841 tgcttccagg aggcgccggc gctccgcgcg gcgctcatcc agctcttttt gggcgagttc
      901 atgcgcggcc acaaactggg ccaattcggc ttcctgcccg gcaatctcct cgcggagggc
      961 acgaacgcgc tcggcgcggc tcgccatgtg cgctcgccgc cgctcgatgt cgcgcagcgt
     1021 ctcctcttcc tggcggtgag cacgcgcggc gttctcgcgc tgcccttcca cctgctgccg
     1081 ggcgcgcgcc gcagccactt tctgatcggc aacggcttcg gcggcacgat cccgttcgcg
     1141 gttgagctcc tccaggctgg cacgcgcgct ctcaatgtcg gcatccgcgc ccgagtcctg
     1201 cgtctgaagc tgctccacgt gcgcgcgcaa gcgcgcctcg cgcgccaccg ccttctcgac
     1261 atctgcctcc gcctggtgga tgctctgctc cgcgcggcgg agatcatccg ccagacgccg
     1321 ggcctcttgc gcgcagtaat cgaggtcttt cttggccgcg gccagcgccg atgcggcgcg
     1381 cgaggcctcc tcacgctgcc ggcgcaggct cacttccgcc gcgcgaaggg cagccgactt
     1441 cgtctcgttc tccttggtga cgcgagccag ctcgttttcg agccgggccg tctcctcttc
     1501 caggccggcc atctcgcgac gccgggccag gagactagcg gcgcccccgt tggcgcgtcc
     1561 accggtgagg ataccgcgcg ggtggagcag gtctccctcc agcgtcacgg cgcggtagcg
     1621 cgcatggtcg agggcaagca cctcgcgcgc ctcctccaaa tggcgcacca cgacggcgtt
     1681 gcccagaacc gccccgatga cattccggtg agcatcggca aagcggacat gctgggtggc
     1741 gcgtcggctc aggatctcct gtgcctccgt gccatcggct acgggcacag cggcttcgcg
     1801 aacgacgaag gtcgcgcggc ccttcccctc tttgcgaagg taggtaatcg cctcgtatgc
     1861 ttcttccagg ctatcgacca caagcgcgtc gaggtcatcc cccagagcgg catggatcgc
     1921 ggcctcaaac tcgggagaga cctccaggag atcgacgatc ggcacgaaat ggcccttcag
     1981 ccgaccctcc ttgcgagccc gcaggatggc gcgcgcgccg tcgcgatacc cctccagctt
     2041 cgcgtcgagc tcgcgcaggg tccgcaggcg cgtggaacgc gccgtgatct ccgtccgcag
     2101 ctccgtcatg cgctgcccgg caaggacgat cccgcgctgc aacgtagcgc gctccgcctc
     2161 ggcgcggcga agctgctcgt tcacctgctc gcatcgccgc tccgcctcgg taaccttctc
     2221 gcgcagtcct tcttctcgcg cctgttgcgt cgccgcccga gcgcccaaca tctcaaggcg
     2281 ctgtgttgcg cgggtgagcg ctgcacgcgc gtcctgaacc tggcgcccac tagcgcgcag
     2341 atccgcctgc gcgttggtga gcgcgcgagc gcgctccacc gcctgacgcc ggtgttcttc
     2401 caccacgcgc gccagcttct ccatgcgccc ctggatcgcc gccacctcag cctcgcagcg
     2461 gcgcagctcc tccgtcgccg cgcgctcggc agcttccgcg gcagcgattc gctcgctcgc
     2521 ctcttccttt tcacggcggc agcgctccag cgcccgctcc agatcggcga tccgctcggc
     2581 atcatccgcc tcgcccgctt ccacggaggc caggcgctcg cgcgagactc ctagagtgcc
     2641 acgcgcgcgc tccaccttct gcgaagcggc cgatgcagcc gcgcgcgcct cct
//
LOCUS       DAOSCV010000629         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24625_length_2688_cov_3.230536, whole genome shotgun sequence.
ACCESSION   DAOSCV010000629 DAOSCV010000000
VERSION     DAOSCV010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24625_length_2688_cov_3.230536"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLY56_14210"
     CDS             complement(<1..632)
                     /locus_tag="PLY56_14210"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HOJ22680.1"
                     /translation="MLCIILLAGGPLLAAPSPHIYIGGREVPCTPSPAVIDGQVYVPV
                     SFHYEGLSVLVRTEDGKVVLRGMGEAEARVPFVMHQGMIYYPIVPACTGIRLGARWVE
                     KTQKLYIAPRIDSIRPEVFDRMLTLRITAGYPVTPKVSVVSDPWRLLIEVPNAQLFTE
                     STTIPIGQGEITRIRAAQYSYEPPMVRIVVDVEGRPQYRVLSGLETTQIRL"
     gene            complement(1041..1389)
                     /gene="ssrA"
                     /locus_tag="PLY56_14215"
     tmRNA           complement(1041..1389)
                     /gene="ssrA"
                     /locus_tag="PLY56_14215"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1594..>2688)
                     /locus_tag="PLY56_14220"
     CDS             complement(1594..>2688)
                     /locus_tag="PLY56_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22681.1"
                     /translation="ANGQAGAFLRPLCTVRADHASEIALDASANHIGIIGPAGQIRIL
                     DATGHPLTDVSVPGADAFAFTPEGQRVVAYARANASHTEVVFMRLDGSVLWRHDPGTA
                     ILDVAVAQSGQRAACVTADGYVYSYQLDEAPSFRRWRSVGNPTGTYVTPDGALVLVAT
                     ADPPSVWAYTPAGVFLWKAPNSDPKGDAQIFASPDGQFLACATMPKDARSGSYTSRFV
                     VLSRSGRQQWGAKLRASHPRVALSTDGKWAAVTYTKSVSTSPEPTYERKVVLFRTSSA
                     GSSVPAQLWQHGGPLYDPRLVCIAGDGSFVATHDGKRSIFFFGPTGQLLWRHKMAHPI
                     VRTCASADGLSILVHCADGSLSLLRVTDAL"
BASE COUNT          513 a          813 c          908 g          454 t
ORIGIN      
        1 agccggattt gcgtggtctc cagaccgctg aggacgcggt attgcggccg gccctccaca
       61 tcgaccacaa tgcgcaccat cggcggctcg taggagtatt gagccgcgcg gatgcgcgtg
      121 atctccccct ggccaatcgg tatggtcgtc gactcggtga agagctgtgc gttgggtacc
      181 tcgatcagca gccgccacgg atcggagacg accgacactt ttggggtgac cgggtagccg
      241 gcggtgatgc gcagcgtcaa catccggtca aacacctccg ggcggatcga atcgatgcgc
      301 ggagcgatgt aaagcttttg cgtcttctca acccaccggg cgccgagacg aatgcccgta
      361 catgctggca cgatggggta gtagatcatt ccctgatgca tgacgaaggg cacgcgcgct
      421 tctgcttccc ccatgccgcg gaggacgacc tttccgtctt cagttcggac cagcacggac
      481 agcccttcgt agtggaagga gacaggcacg tagacctgcc cgtctatcac cgccggcgat
      541 ggggtgcagg gaacctcccg gccgccgatg tagatatgag gcgatggcgc ggccagaagc
      601 ggcccacccg ccaggaggat gatgcagagc aacgcgagac gaattggtag acgtggaatc
      661 gtgacggcac ctccttacac aaacggtgac tcctgtgacg agcctccggc cgatattgtt
      721 cgataaggga gcagagtcaa tgcctctgcg cgtgttctgg tatgaaggta ccgcctccgc
      781 gagtgcccaa agtgaattca aaaggaagat gatcgcggta agggcagcgg catgatatga
      841 ttcagagaaa gaatgtaatg gtccacgctg gcgatgcacg tggaagagga ggcgctaggg
      901 cagggaagaa cggcaggcaa gccgcgagaa gctcatcgct cctaatggcc gccagctgcc
      961 ccgccggcat gccgtctcgg tgacggggaa cgcacccgca cagcggcaga ccagcccctc
     1021 gcgccttgat gcgttgtctt tggtggaggc gggcggagtt gaaccgccgt ccaaagggag
     1081 atcggcagag acccctacga gcgtagtctg cgtttttcgt ttcgccccat cggctcccgc
     1141 agacgggatc ctccggcgct aggtcgctaa atgtccctgg cagcccgcgt accctgacac
     1201 gccaggtaat ccggctttgg taacacccag taccgatccc gccggatggg gatcggccgg
     1261 atggctgccc ttaggcagcg agtgcgagtt gattctcggc gtttgtttgt ttgccgcttt
     1321 ttacgaggcc agcggcgcct cggctcgcat tctctgtctc tctcgcccct gtcgaaaccc
     1381 gtcgccccca cgaatcgaat tggcaacgga ccaaactcgc atcctcatta taccatacga
     1441 ggccatgtga cgcaaacagt gataacgcga ttctgagctt tcccgtcgtc cggcccctcc
     1501 agagagactc tcgcactcac tcgggtcgcg aaccggggcg agacggccca ggggaaggtc
     1561 aggacgctgc gggtgaggtg gcctgctggc cggctagagg gcgtccgtga cgcggagcaa
     1621 tgagagcgag ccatcggcgc agtggacgag gatggagagc ccatccgccg aggcgcaggt
     1681 gcgtacgatg ggatgtgcca tcttgtgccg ccaaaggagc tgtccggtgg ggccgaagaa
     1741 gaagatgctc cgcttcccat cgtgggtggc cacgaaggag ccgtcgccgg caatgcagac
     1801 gaggcgcggg tcatagagcg ggccgccgtg ctgccagagc tgggcaggga cgctggagcc
     1861 cgcgctgctc gttctgaaga gcaccacctt ccgctcgtag gtgggttccg ggctggtgga
     1921 aacactcttg gtataggtta ccgccgccca cttgccatcg gtagagagcg ccacccgggg
     1981 atgtgaggcg cgcagtttgg cgccccactg ctggcgacca ctgcggctga gaacgacgaa
     2041 gcggctggtg tagctgcccg agcgagcatc cttgggcatc gtggcgcacg ccagaaactg
     2101 cccatccgga ctcgcgaaga tctgcgcatc gcccttggga tcactgttcg gcgccttcca
     2161 gaggaaaacg cccgccggcg tgtaggccca cacgctgggc ggatcggcgg tggcgacgag
     2221 cacgagcgcc ccgtcggggg tgacataggt gccggtggga ttgcccacgc tgcgccagcg
     2281 ccggaagctg ggcgcctcat ccagctggta cgaatagacg tagccatcgg cggtaacgca
     2341 cgcggcgcgc tggccagatt gggcgactgc cacgtccaga atcgccgtgc cgggatcgtg
     2401 ccgccagagt accgagccat cgagacgcat gaaaacgacc tcggtatggg atgcgttggc
     2461 acgcgcgtag gcaacgacgc gttggccctc gggggtgaaa gcgaaggcgt ccgcgccggg
     2521 aaccgaaacg tccgtgaggg ggtggccggt cgcatccagg atgcgaatct ggcccgccgg
     2581 gccgatgatg ccgatgtggt ttgccgaagc atccagggcg atctcggagg cgtgatccgc
     2641 gcgcacagtg caaagcgggc gcaggaacgc cccggcctgc ccattggc
//
LOCUS       DAOSCV010000630         2681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24704_length_2681_cov_3.015994, whole genome shotgun sequence.
ACCESSION   DAOSCV010000630 DAOSCV010000000
VERSION     DAOSCV010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24704_length_2681_cov_3.015994"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..920
                     /locus_tag="PLY56_14225"
     CDS             63..920
                     /locus_tag="PLY56_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ22682.1"
                     /translation="MAGFLFIAPWLAGFLLLTAGPVVASILLSFCEYDVLHPVAWAGL
                     DNYRALPRDPLFWKSLGNTLFMVLGVPLGMAVSLGVAMLLNARVKGMAFYRTIYYLPA
                     IVPAVASSILWIWVLNPQFGLVNVALQRLFGITGPQWLQSELWSKPSILLMGLWGAGA
                     GMIIWLAGLKGIPETLYEAAEIDGAGGWAAFRHVTIPMLSPYIFFNLIMGVIGTFQIF
                     TQAYIMTGGGPVDSTLFYVYYLFNNAFRYFKMGYASALAWILFVIILALTLLQLRLGK
                     RWVYYEGEK"
     gene            904..>2681
                     /locus_tag="PLY56_14230"
     CDS             904..>2681
                     /locus_tag="PLY56_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ22683.1"
                     /translation="MRGRSRGEGKGRPGSRIEPAHSARARRRRCEWIRQAGIHLALLA
                     GCVLFAFPFVWLVTTSFKDEREIAAFPPRWIPRVPHYSSRSPFVDPDYYDEMKRPAGM
                     APEMWREHRDAIARWLDTCLASACSLHPEALPVADVVTDPTWERALRNGMWDELSRIL
                     PDEVAQQPDRLKTFIAANVSAERLRKVATGLSRVCAIGELSLRDARHDRVDSTAESGR
                     WRVVSGAAELGRRVAVEIESGQLLRYDLGAGPVLLERSLYLSRPGALGEWVDSITLGL
                     HSDDSWHRLFLTVETPDAVYRSEEAFWLERASWRDLVWRFSEPDDRTKPVVVLRPDPR
                     ARATSLAANEIRLRLRIERTSPLMAAYAKYSLNYRRALRYIPFWRYVGNSVYLVILNI
                     LGQVLACSLVAYAFARLRFYGRDVLFALLLATMMLPGQVTMIPVFLIFKSLGWYDTLK
                     PLWVGAWFGSAFYIFLLRQFFLGIPTDLEDAAKIDGCGFFGLYWRIMLPLVRPALATV
                     AIFTFMSTWNDFMGPLIYIGREELSPLALGLFQFRRAWGRVGHDDGRQHLDDAARGGD
                     LLPGPALLHPGRHAHRRRHDRRHDAQG"
BASE COUNT          377 a          897 c          876 g          531 t
ORIGIN      
        1 tgttcgggtg ggggcttcgc cgcgacctcc gccagcggcg caggcccggg cgccacgaga
       61 ccctggcggg cttcctcttc attgctccct ggctcgccgg gttcctcctt ctcaccgccg
      121 gtccggtggt cgcctcgatc ctgctctctt tctgcgagta cgacgtgctc cacccggtgg
      181 cgtgggccgg cctggacaac taccgcgccc tcccgcgcga cccgctcttc tggaagtcgc
      241 tcggaaacac tcttttcatg gtgctcggcg tgcctctcgg catggcggtg agcctgggcg
      301 tggcgatgct cctgaacgcg cgcgtgaagg ggatggcgtt ttaccggacg atctactacc
      361 tgccggcgat tgtgcccgcg gtggcgagca gcatcctctg gatctgggtg ctcaaccctc
      421 agttcggact ggtgaacgtc gccctccagc gcctcttcgg gatcaccggg ccgcagtggc
      481 tgcagagcga gctttggtcg aaaccctcga ttctcctgat gggcctctgg ggtgccgggg
      541 cagggatgat catctggctg gccggcctga aggggattcc cgagacgctc tacgaggccg
      601 ccgaaatcga cggcgcgggc gggtgggccg ccttccggca cgtgaccatc ccgatgctct
      661 cgccctacat cttcttcaac ctgatcatgg gcgtcatcgg cacattccag atcttcacgc
      721 aggcctacat catgaccggc ggtggcccag tcgactccac gctcttctac gtctactacc
      781 tcttcaacaa cgccttccgc tacttcaaaa tgggctacgc ctcggcgctc gcctggatcc
      841 tcttcgtgat catcctggcg ctgacgctgc tccagctgcg gcttgggaaa cggtgggtgt
      901 actatgaggg ggagaagtag aggcgagggg aaggggaggc ccgggtcgcg gatcgagccg
      961 gcgcactcgg cgcgcgctcg ccgacgccgg tgcgaatgga tccggcaggc cggcatccat
     1021 ctcgcgctcc ttgccggatg cgtccttttc gcgttcccct tcgtctggct ggttacgacc
     1081 tcgttcaagg acgagcggga gattgctgcc ttcccgcccc ggtggatccc gcgggtgcct
     1141 cactattcct cgcgctctcc cttcgtggac cccgattact acgacgagat gaagcgcccc
     1201 gccggaatgg ccccggagat gtggcgcgag caccgcgacg cgattgcccg gtggctggat
     1261 acctgcctgg ccagcgcctg cagccttcac cccgaggcgc tgccggtagc tgacgtggtg
     1321 accgatccca cctgggagcg cgctctgcgc aacggcatgt gggacgagct gtcgcgcatc
     1381 ctgcccgatg aggtggcgca acagccggac cggctgaaga cgttcatcgc tgcgaacgtg
     1441 agcgccgaac gcctgcgcaa agtggccaca gggctctcac gcgtctgcgc cattggtgag
     1501 ctgagcctgc gcgacgcccg ccacgaccgg gtcgattcca ccgccgagag tggccggtgg
     1561 cgggtggtct ccggtgcggc cgagctgggc aggcgggtgg cggtggagat cgagtccggt
     1621 cagctgctac gctacgactt gggcgctgga ccggttctcc tggagcgctc tctctatctg
     1681 tcccggccgg gcgcgctggg ggagtgggta gactccatca cgctcgggct gcactcggat
     1741 gactcgtggc accgcctctt cctgacagtg gagacgccgg acgcggtcta tcgctccgaa
     1801 gaggcgtttt ggctggaacg cgcttcctgg cgcgacctgg tgtggcgctt ctccgagcca
     1861 gacgaccgta cgaagccggt ggtggtgttg cgccccgacc cgcgcgcgcg agccacgtcc
     1921 ctcgccgcga acgagattcg gctgcgcctg cggatcgagc ggacctcgcc tctgatggcg
     1981 gcctacgcga agtactcgct caactaccgg cgggcgctgc gctacatccc gttctggcgc
     2041 tacgtgggga acagcgtcta cctggtgatc ctcaacatcc tggggcaggt gctggcgtgc
     2101 agcttggtcg cctatgcctt cgctcggctc cgcttctacg ggcgcgacgt tctcttcgcc
     2161 ctcctgctgg cgacaatgat gctgccgggg caggtgacga tgatccccgt cttcctcatc
     2221 ttcaagagcc tggggtggta cgacacgctg aagccgcttt gggtgggagc ctggttcgga
     2281 agcgcgttct acatcttcct gctccgccag ttcttcctcg ggatcccgac ggacctggag
     2341 gacgccgcca agatcgatgg ctgcggcttc ttcgggctct actggcggat catgctcccg
     2401 ctggtgcgcc cggcgctcgc gacggtggcg atcttcacct tcatgagcac ctggaacgac
     2461 ttcatgggcc cgctcatcta catcgggcgc gaggagctat ctccactcgc attggggctc
     2521 ttccaattcc gtagagcatg ggggagagtg gggcatgatg atggccgcca gcaccttgat
     2581 gacgctgccc gtggtggtga tcttcttcct ggcccagcgc tacttcatcc agggcgtcac
     2641 gctcaccgcc gacgccacga tcggcgacac gacgctcaag g
//
LOCUS       DAOSCV010000631         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24723_length_2680_cov_3.061714, whole genome shotgun sequence.
ACCESSION   DAOSCV010000631 DAOSCV010000000
VERSION     DAOSCV010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24723_length_2680_cov_3.061714"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..891
                     /locus_tag="PLY56_14235"
     CDS             37..891
                     /locus_tag="PLY56_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ22684.1"
                     /translation="MSPWLLGFLIFTAGPMLTSLYLSFCKYDLHTLTWVGTKNYEVLL
                     TRDAGGLFWKSLTNTALYVLFSVPLGLTGSLLVAMLLNQKVKGIALFRTLFYLPSLVP
                     AVASALIWQWVFHPDAGLLNYGLSKVGVDGPDWLQDPKTALSSLIIMSLWGIGGGRMI
                     IFLAGLQGISDELYEASSLDGAGAWHQFRHVTLPMLSPTIFFNLVLGIIGSFQVFTSA
                     YVMTGGGPNNATLMYVLYLYRNAFVYFKLGKASAMAWILFVILLVFTIIQFKSASKWV
                     YYEGAERE"
     gene            901..1758
                     /locus_tag="PLY56_14240"
     CDS             901..1758
                     /locus_tag="PLY56_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ22685.1"
                     /translation="MAQTATSPARQGTRWGRVVTFVLLCLGSAIFSIPFIWTVSTALK
                     TKEQVFAVPPVWIPNPVQWENFRRAWTELPFPTFVFNTVLITLISVVGQVTSASLVAY
                     GFARFRFKGRTFLFYLLLSTMMLPAQVTMIPVFLIWRALGLIDTFAPMTVPAFFGGGA
                     FTIFLLRQFFMTVPRELDEAAMIDGCSYFGIWWRILLPLSRPALVTVTLFSFLAHWDD
                     FMGPLIYLNSMEKYTVSIGLRMFQDMFGTELELLMAASLIHILPTIVLFFIGQRYFIK
                     GIALTGLKG"
     gene            complement(1972..>2680)
                     /locus_tag="PLY56_14245"
     CDS             complement(1972..>2680)
                     /locus_tag="PLY56_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018755875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOJ22686.1"
                     /translation="ADLYGLGEIFDPAQARSALEAIFRHNFKKPIRDYYNPWRVFCVN
                     DEAGLVICDYPEGRRKPRIPLTYAQETMHGFEYAAAILMILRGLVEEGLTVVDAVRER
                     YDGEKRNPWNEIECGSNYARSMASYALLNAFSGFEFNLPAGHIGFRPLRLVDGRFRCF
                     WSLDPAWGEVLFSPEGAEVRVLYGSLSLRSLGLPFVPASVEVAGQPVGFVVRDGALHF
                     GEPVTLDRRRWLRVKPR"
BASE COUNT          442 a          912 c          777 g          549 t
ORIGIN      
        1 ctcttcgacc ggcctgcgtt ctggggatac ctctttatct caccctggct gctcggcttc
       61 ctcatcttca ccgccggccc catgctcacc tcgctctacc tgtcgttctg caagtacgac
      121 ctacacacgc tcacctgggt gggcaccaag aactacgagg tgctcctcac ccgcgatgcc
      181 ggcgggctct tctggaaatc gcttacgaat acggcactct acgtcctttt ctcggtgcca
      241 ctcggtctga caggctcgct gctcgtcgcc atgctgctca accagaaggt gaaggggatc
      301 gccctctttc gcaccctctt ctacctgccc tcgctggtgc cggccgtcgc ctcggcgctc
      361 atctggcaat gggttttcca tcccgatgcc gggttgctga actacggcct cagcaaggtg
      421 ggcgtggacg gccccgactg gctccaggat ccgaagacgg cgctctcctc gctgatcatc
      481 atgagccttt ggggcattgg tggcgggcga atgattatct tcctggccgg gctgcagggg
      541 atctcggacg agctgtacga ggcgtcgtcg ctggatggcg ccggcgcgtg gcatcagttc
      601 cggcacgtga ccctgccgat gctctccccc accatcttct tcaacctggt actcgggatc
      661 atcggctcgt tccaggtctt cacctccgcc tacgtgatga ccggtggcgg ccccaacaac
      721 gccacgctga tgtacgtgct ctatctctat cgaaacgcct tcgtctactt caagctgggc
      781 aaggcgtcgg caatggcgtg gatcctcttt gtgatcctcc tcgtcttcac gatcatccag
      841 ttcaagagcg ccagcaagtg ggtctactac gaaggcgcgg agagggagtg agagattacg
      901 atggcgcaaa cagcaacatc acccgcgcgt caaggcaccc ggtggggccg ggttgtgacg
      961 ttcgtgctcc tctgcctggg gagcgcgatc ttctcgatcc ccttcatctg gacggtctca
     1021 acggcgctca agactaagga gcaggtcttt gccgtgcccc cggtgtggat ccccaatccc
     1081 gtgcagtggg agaacttccg ccgcgcctgg accgagcttc cttttcccac cttcgtcttc
     1141 aacacggtgc tgatcacgct catctccgtg gtggggcagg tcacgtccgc gtcgctcgtg
     1201 gcctacggct tcgcacgctt ccgcttcaaa ggccgcacct tcctcttcta cctgctcctc
     1261 tccacgatga tgctgcccgc ccaggtgacg atgatcccgg tcttcctcat ctggcgcgcc
     1321 ctgggtctga tcgacacctt cgcgccgatg accgtgcccg cgttcttcgg cggcggtgcc
     1381 ttcaccatct tcctcctgcg ccagttcttc atgaccgtgc ccagggaact ggacgaggcc
     1441 gcgatgatcg atggatgcag ctatttcggc atctggtggc gcatcctcct cccgctctcc
     1501 cggccggcgc tcgtcaccgt gacgctcttc tccttcctgg cccactggga cgacttcatg
     1561 ggccccctga tctacctcaa ctcgatggag aagtacaccg tctcgattgg cctgcgcatg
     1621 ttccaggata tgttcggcac cgagctggag ctactgatgg ccgcgtcgct catccatatc
     1681 ctgccgacca tcgtgctctt cttcatcggc cagcgctact tcatcaaggg gatcgcgctc
     1741 accggcctga agggctgagt tcggggcggc ggccgacggg agccgccgcc tcacttcgcg
     1801 cttagcccgc attattcatc agaataatga aaagaagtct ctctgccagg cgtggcccgg
     1861 attcatgggc actgctcccg aaaacactct gccgccgggc gcttgtagcg gcaacggcgt
     1921 atcaccagac aagagacttc tcttgtgaag tattctggtg aatacttcgg gttagcgcgg
     1981 cttcacccgc agccagcgcc gcctgtccag ggtaaccggc tccccgaagt ggagcgcgcc
     2041 atcccggacg acgaagccga ccggctggcc ggccacctcg acggacgccg gcacaaacgg
     2101 taggcccagg ctccgcagcg agagcgagcc gtagaggacg cgcacctctg cgccctcagg
     2161 agagaagagg acttcgcccc aggcaggatc gagcgaccag aagcagcgga accggccatc
     2221 caccagccgc agcggccgga agccaatatg cccggcaggc aggttgaact cgaagccgct
     2281 gaaggcgttg aggagggcgt agctcgccat cgagcgggcg tagttactcc cgcattcgat
     2341 ctcgttccag gggttgcgct tctcgccgtc gtagcgctcg cgcacggcat ccaccaccgt
     2401 caggccctcc tccaccagcc cgcgcaggat catcaggatg gcggcggcat actcgaagcc
     2461 gtgcatcgtc tcctgcgcat aggtcagcgg tatccgcggc ttgcgccggc cctcgggata
     2521 gtcgcagatc accaggccgg cctcgtcgtt cacgcagaag acgcgccagg ggttgtagta
     2581 atcgcggatc ggcttcttga agttgtgccg gaagatcgcc tccagcgccg agcgcgcctg
     2641 cgcggggtcg aagatctcgc ccaggccata gaggtccgcg
//
LOCUS       DAOSCV010000632         2674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24795_length_2674_cov_3.269187, whole genome shotgun sequence.
ACCESSION   DAOSCV010000632 DAOSCV010000000
VERSION     DAOSCV010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24795_length_2674_cov_3.269187"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLY56_14250"
     CDS             <1..540
                     /locus_tag="PLY56_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22687.1"
                     /translation="GQVSARVAPGLSISRERLGIDYARCWVRAALRAIRCDTGEVVMA
                     DTVAGEGDGVPTQKAVHAGMTHTLRRAGRKLSESLLRLPGAASRAMCLTLSGFEGVTD
                     ATAFIEALSSVPAIRRVEGQTYTEGVLTLQVRVAEEAAASLGAHLESSAALQAFGLRV
                     IRATPERVSGSVYAAETTG"
     gene            complement(683..898)
                     /locus_tag="PLY56_14255"
     CDS             complement(683..898)
                     /locus_tag="PLY56_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22688.1"
                     /translation="MSEKNGGVNTGLAVVIIAVIVLAIGFFYWHGSTPKPAEEIPGPP
                     MSQMMGNMPGSGPEGKPANAPAAPAKP"
     gene            1352..>2674
                     /locus_tag="PLY56_14260"
     CDS             1352..>2674
                     /locus_tag="PLY56_14260"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOJ22689.1"
                     /translation="MSIWRVLAIAAIAVLSAGAPTRAQGFTGPQLKVESVASVDRIRP
                     GDSFQIAIILSLPPGLHVNAHLPSDPDLIPTVVEITPPPGIEVGAVEYPAARERALSF
                     TPKPIAVYEGEVVMLAPATASANLARAPLVLEGTLTAQACNDESCFPPSTVPFSLKLA
                     VDVAGAAVKPVRPELFAAGATMGPGEEGTPQSGAIAAAGSRAHYSRLTKFVPPDEFVH
                     WLEEGAPAEEKSRLSDLILGGHWLLALPLIYLAGLALNLTPCVYPLIPITIGFFGGQS
                     GGSRRHLLSLASLYVLGMALMYSTLGVVAAMNRQLFGNQLQNPYVLLGFAAVMIALGL
                     SMFGVWEFRVPAGLAGRVSGRAGFAGALIMGLLVGFVAAPCIGPVVVALLAVVSQLGH
                     PGMGFGIFFALALGLGTPYLLLGVFSGLIQRLPRSGEWMNAVKHIFGLI"
BASE COUNT          399 a          886 c          893 g          496 t
ORIGIN      
        1 gggcaggtct ccgcccgagt ggcacccggg ctttcgatat cccgcgagcg gctgggaatc
       61 gactatgccc ggtgttgggt gcgagcggca ctgcgcgcca ttcgctgcga tacgggggaa
      121 gtggtgatgg cggatacggt ggcgggcgag ggtgacggcg tgcccacgca gaaggcggtt
      181 catgccggaa tgacgcacac cctgcgccgc gctggccgca agctatccga gtcgctgctg
      241 cgcctgccgg gagctgcctc acgcgccatg tgcctcaccc tctccgggtt cgaaggcgtg
      301 accgatgcga ctgccttcat cgaggcgctc tcctccgtgc ccgccatccg ccgggtggaa
      361 ggccagacct acaccgaggg cgtgctcacc cttcaggtga gggtcgcgga ggaggccgct
      421 gccagcctgg gcgcgcacct cgagagctcg gcagcactgc aggcctttgg attgcgcgtg
      481 atccgcgcca cgccggagcg cgtctccggc tctgtctacg cggcggaaac gaccgggtga
      541 gcgacaccgg cacgtctctc cgccgtggag gcgcgccatg tcagcactcc tgcaggggct
      601 gcccagaaag ggcggcccct gcgagagcat ccggctacaa agctcacgag cgtcagatcc
      661 ccaggaagca caccggcacg cttcaaggct tcgcaggcgc cgccggtgcg ttggccggtt
      721 tgccttccgg accgctcccg ggcatgttgc ccatcatctg cgacatgggc ggcccaggga
      781 tctcctccgc aggcttgggc gtgctgccat gccagtagaa gaagccgatg gcgagcacga
      841 tcaccgcgat gattaccacc gcgagacccg tgttgacgcc gccgtttttc tcactcatgg
      901 tggtctctcc tccatatttc gatgctcgca aatcccgcgg actcggctcc aaccaggcgc
      961 gcgcaccgta tccgagcggg gcatccatcc agtgaaggcg tagaggtcgc tggagtcagc
     1021 cgcgctctgg atagcggaag ccatcgagag agttcccctc ttcatgacat ccgcgagatc
     1081 ggcctgccct cttgcatggg ggcctcatcg gcgagagtgg tacgctgttc gttgacaccg
     1141 cgcgcctctc cttccggctc cgcgcgccag gcagccggcg attctctccc caggaggcgc
     1201 aacctctggg cctgagcagg tatctttctc tatggagcct cgcgccgggc gcccgcaccg
     1261 ccgcacctga gaggtcacgc ggtggctgtc ggaacaaagg ggcggtagcc caagcccgtg
     1321 cggttgccca cccgggagga gaggagcgac tatgtcgatc tggagagtac tcgccatcgc
     1381 cgccatcgcg gtcttgtccg cgggcgcgcc tacccgcgct cagggcttca cagggccgca
     1441 gctgaaggtt gagagcgtgg cctccgtgga tcgcatccgc ccaggggatt ccttccagat
     1501 cgccatcatc ctgagcctcc cgcccgggct gcacgtgaac gcgcacctgc cgagcgatcc
     1561 ggatctcatc cccacggtgg tggagatcac gccgcccccg ggaatcgaag tgggcgcggt
     1621 ggagtatccc gcggccaggg agcgggctct ctccttcaca cccaagccga ttgcggttta
     1681 cgagggcgaa gtggtgatgc tggcgccggc aacagcatcc gcgaacctcg cgcgagctcc
     1741 attggtgctg gagggcaccc tcaccgcgca ggcatgcaac gatgagtcgt gctttccccc
     1801 atccacagtg cccttctcgc tcaagctggc ggtggatgtc gccggcgcag cggtgaagcc
     1861 agtgcgcccg gagctgtttg ccgcgggagc gacgatgggg ccgggagaag aaggcacgcc
     1921 ccagtctgga gccatagcgg cagccggcag tcgggcgcac tattcccggc tgacgaagtt
     1981 cgtccccccc gatgaattcg tgcattggct ggaagagggc gctcctgccg aggagaagag
     2041 ccgcttgagc gacctgattc tggggggcca ttggcttctt gcattgccgc tcatctatct
     2101 tgcggggctg gctttgaacc tgacgccctg cgtctacccc ctgatcccga tcaccatcgg
     2161 cttttttggc gggcagagcg gcggaagccg ccggcatctg ctgagcctgg cctcgctcta
     2221 cgtgctggga atggcgctga tgtactccac gctgggcgtc gttgcggcca tgaaccggca
     2281 actctttgga aaccagcttc agaacccgta tgtgctgctg ggcttcgccg cggtgatgat
     2341 cgcgctgggg ctttccatgt ttggtgtgtg ggagttccgc gtgccggcag ggctggctgg
     2401 ccgggtttcc ggccgcgcgg gcttcgcagg cgcgctgatc atgggacttc tcgtcggctt
     2461 tgttgcggcg ccctgtatcg gtccggtcgt cgtggcgctc ctggcggtcg ttagccagct
     2521 cggccatcca gggatgggct ttggcatctt cttcgcgctc gctctggggc tgggaacgcc
     2581 ctatctcctc cttggcgtct tctcggggct catccagcgc ctgccccgct ccggcgagtg
     2641 gatgaacgca gtgaagcaca tcttcggact gatc
//
LOCUS       DAOSCV010000633         2672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24815_length_2672_cov_3.340848, whole genome shotgun sequence.
ACCESSION   DAOSCV010000633 DAOSCV010000000
VERSION     DAOSCV010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24815_length_2672_cov_3.340848"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2672
                     /locus_tag="PLY56_14265"
     CDS             <1..>2672
                     /locus_tag="PLY56_14265"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ22690.1"
                     /translation="QILWNIHATDRYEEYVALFTAPDPACRLYLYNVAKTKGTVSYDH
                     VSLREGAPDRPIFTPLTWKPVDRPLTDPPATPHLDWASPLAGGALKTLITLRTTRGLR
                     QAAELAQRIDLDYDVVDTGYEGRELISETARRIANRLKDRDYHVYLVASRVPEALAKE
                     IRQQVEAGAGLVVLEGFGQTGKIVPLSNLKEVAANHYVREGIPWDLVPEKILTSVQTG
                     TLGKGRVVRLVFPMDKGRVWGLLPQDLDYTAYRTRQCAYWEWWFSLLSRALVWTSGRE
                     GNTRLQAEVAPGEIRFRVANAPAGSRLRAVVRSGRELRFDGPLLRQAPVTVPVTANGT
                     ARLALPETLPAGTVLVDAMALNQAGEVIAWGSFPVARPQAVRISALETASESYRIGEP
                     AQATLRCAADAPVDAVVEARLIDAFGRVVGRQSISRRLSRGESRFTLALRPARPLTVL
                     HRIFARILVGGKEQDSRWVAVTWPKVGPARAAADFVATTWAPGMSHPLELEYYGERTR
                     DLGLNSEFAISPYAIAEHAMLGGGYMGAAGFRETGWNETGIRPRCLSDPAVIEAYRQK
                     AREAAASQRPFGFFGVGITDEAFLTSRHRRYEVCFSDHCQARYRRWLQKRYPSLDALN
                     ARWGTAYRSWEEIRGARTEEVRGKENYAPFVEFRTFMTDVWVEACRDVTAAYHEVAPE
                     TPTGHTNTFGADPFNGNDYWKLATATGFGWGQEYSEAIKRSAHKAIFDLWRSFVETPQ
                     ARESRRRPGMRDVPPFHNYGWIGYDHRVEAAHYEPWWLALHGARGVSYYATNAVDSGR
                     GVSWALIHPTLSDTGYGQAVKEALRDLRAGCGKLFLEYQREEPRIALLWSYPSMLVSW
                     CESSWTEPEPDERDGTDSYGSYFRSALHFRQHVN"
BASE COUNT          433 a          929 c          888 g          422 t
ORIGIN      
        1 gcagatcctc tggaacatcc acgccacgga tcgctacgag gagtacgtcg ccctcttcac
       61 ggccccggat ccggcctgcc ggctctacct ctataacgtc gccaagacca agggcaccgt
      121 ctcctacgac cacgtctcgc tccgcgaggg agcgccggat cgcccgatct tcacgccgct
      181 cacctggaag ccggtcgacc ggcccctcac cgatccacca gccacgccgc atctcgactg
      241 ggcttcgccc ctggcgggcg gggcgctgaa gacgctgatc accctccgca caactcgcgg
      301 cctccggcaa gctgccgagc tcgcccagcg catcgacctc gactatgacg tggtggatac
      361 cggctatgag ggccgcgagc tcatctcgga gacagcccgg cggatcgcca accggctgaa
      421 ggatcgcgac taccacgtct acctggtggc tagccgcgtg cccgaggcac tggcgaaaga
      481 gatccgccag caggtggagg ccggcgccgg actcgtcgtc ctggagggct tcgggcaaac
      541 gggcaagatc gtgccgctca gcaacctcaa ggaggtggcc gccaaccact acgtgcgcga
      601 agggattccg tgggatctgg ttcccgagaa gatcctcacc tcggtgcaga cgggcaccct
      661 gggcaaaggg cgcgtggtgc gcctggtctt tccgatggac aaggggcgcg tgtggggcct
      721 tctcccccag gatctggact acaccgccta ccgaacacga cagtgcgcct actgggagtg
      781 gtggttctcg ctcctgtcgc gcgcgctagt ctggacatcc ggccgggagg gcaacacccg
      841 cttacaggca gaggtggctc ctggcgagat ccgcttccgc gtggccaatg cgcccgcggg
      901 atcgcgcctc cgggccgtcg tgcgcagcgg tcgggagctc cgtttcgatg gccccctgct
      961 ccgccaggca cccgttacgg tgccggtcac ggcgaatgga accgcgaggt tggctctccc
     1021 cgagactctc ccagccggta cggtgctggt ggacgcgatg gcgctcaacc aggccggcga
     1081 ggtgatcgcc tggggcagct tcccggtcgc gcggccccag gctgtccgga tctccgccct
     1141 ggagacagcg agcgagagct atcggattgg cgagccggcc caggccacgc ttcgctgtgc
     1201 cgccgatgca ccggtcgacg cggtagtgga ggcacgcctc atcgatgcct tcgggagggt
     1261 ggttggccgg caatcgatct cgcgccggct ctcccggggc gagagccgct ttaccctggc
     1321 gctgcggccg gcacggccgc tcaccgtcct ccaccggatc ttcgcgcgca tcctggttgg
     1381 cgggaaagag caagacagcc gctgggtagc cgtcacatgg ccgaaggtgg gcccggcgcg
     1441 cgccgccgca gacttcgtgg cgaccacctg ggcgcccggc atgtctcacc ccctggagct
     1501 ggagtactac ggcgagcgca cgcgcgacct cggcctcaac tccgagtttg cgatcagccc
     1561 gtacgcgatc gcggagcacg ccatgctggg aggcggctac atgggcgccg cggggttccg
     1621 tgagaccggc tggaacgaga cggggatccg tccgaggtgc ctcagcgacc cggcggtgat
     1681 cgaggcctac cgccagaagg cacgcgaggc ggcagcgagc cagcgcccct tcggcttctt
     1741 cggcgtggga atcaccgatg aagcgttcct cacctcgcgg caccggcgct atgaggtctg
     1801 cttcagcgac cactgtcagg cgcgctaccg acgctggctg cagaagcgct acccctcgct
     1861 ggacgcgctc aacgccagat ggggcacggc ctatcgctcc tgggaggaga tccgcggtgc
     1921 gcgcaccgag gaggtgcgtg gcaaggagaa ctacgcgcct ttcgtcgagt ttcgcacctt
     1981 catgacggat gtctgggtgg aggcctgccg cgatgtgacc gccgcctatc atgaggtggc
     2041 gccggagacg ccgacaggac acaccaacac cttcggcgcg gatcccttca acggcaacga
     2101 ctattggaag ctcgccacgg cgaccggctt cggctggggc caggagtact ccgaggcgat
     2161 caagcgctcg gcgcacaagg cgatcttcga cctgtggcgt agcttcgtag agacgccgca
     2221 agccagggaa tctcgccgcc gccccggcat gcgcgacgtg ccgcccttcc acaactacgg
     2281 ctggatcggc tacgatcacc gcgtcgaagc cgcgcattac gagccgtggt ggcttgctct
     2341 gcatggcgcc cgaggcgtga gctactatgc cacgaacgcc gtcgatagcg ggcggggtgt
     2401 ctcctgggcg ttgatccacc ccacgctcag cgacaccggc tatggccagg ccgtgaagga
     2461 ggcgctccgc gatctgcgcg ccggctgcgg caagctgttc ctcgaatacc agcgcgagga
     2521 gccacgcatc gcgctcctct ggtcctaccc ctccatgctc gtatcgtggt gcgagtcgtc
     2581 ctggaccgag cccgagccgg atgagcgtga cggcaccgac agctatggct cttacttccg
     2641 gagcgccctc catttccgcc agcatgtgaa cg
//
LOCUS       DAOSCV010000634         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24854_length_2670_cov_3.017208, whole genome shotgun sequence.
ACCESSION   DAOSCV010000634 DAOSCV010000000
VERSION     DAOSCV010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24854_length_2670_cov_3.017208"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..383
                     /locus_tag="PLY56_14270"
     CDS             177..383
                     /locus_tag="PLY56_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22691.1"
                     /translation="MAVVRVNVKEHYGTGEVWSDAGNETAAAEDVRQWCEENPPWEMR
                     NLEPVRGDDGRYKFAVVRAYGRVT"
     gene            385..636
                     /locus_tag="PLY56_14275"
     CDS             385..636
                     /locus_tag="PLY56_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22692.1"
                     /translation="MVLHKIEEIADEESRLQQLRSLVDRAQEEIRTGKIASEEVEQVI
                     AGLRRDAERLFPGTGNTFDLIYRPRLERAAREAQERVAV"
     gene            679..939
                     /locus_tag="PLY56_14280"
     CDS             679..939
                     /locus_tag="PLY56_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOJ22693.1"
                     /translation="MGGERRVPENIVVYVRPNCSECEQVIRYLENRGLRFRVRDVERD
                     ADALGDLDALGYTDVPVTFLDEEVVVGFDTNRLDASLVNITV"
     gene            969..1940
                     /locus_tag="PLY56_14285"
     CDS             969..1940
                     /locus_tag="PLY56_14285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ22694.1"
                     /translation="MSEVDISVVVPVFNEEGCLAELHRRLTAVLRKVAGSYEMVFVDD
                     GSRDGSLALLRELHAGDREHVRVISLSRNFGHEMASTAGLRYARGRAVVLIDADLQDP
                     PELIPELVARWKEGYDLVYAQRATRARETWLKRATSFAFYRVMRRLANLDLPRDTGDF
                     RLMDRKVVDAFNRCSERNRFVRGLICWVGFRQTGVKFHREPRFAGRTKYNYFKLTRLA
                     IDSIVAFSIVPLRLATLLGGVIGIGSFVMFLLVLLEKLRGLGPERGYALLGTGVLLLG
                     SVQIFLLGIIGEYIGRIYQETQRRPLFLVREEIGYQESPAADPAFFM"
     gene            2056..>2670
                     /locus_tag="PLY56_14290"
     CDS             2056..>2670
                     /locus_tag="PLY56_14290"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ22695.1"
                     /translation="MLKITFLGTGTSHGVPVIGCDCPTCTSTDPRNHRTRCSILVEQN
                     GASVLVDTSPELRLQAIRAGLKRVDAVLFTHAHADHVFGLDDVRRFNDLQGGALPCYG
                     DSTTLRELRRIFRYAFVPTQVGGGKPRLELRRLLRPSFNLHGLEVRPLRVMHGQLPIT
                     AYRFGKSAYVTDASFLPEETVAQLQGLDLLILDACAAGHTRRTST"
BASE COUNT          439 a          832 c          893 g          506 t
ORIGIN      
        1 cggtctcttc ccgcaaggga gagttgccgg catgtggcaa cgcccttgca tcgcgaacaa
       61 cggcccgcgt cggtgcgtcg tacagacggc cggtggggtc accgctcttc aggcatcgcc
      121 cgctgtgccg tttcgcgagc ctgcgctctg ggtatctggc gtgcaggagg ggcacgatgg
      181 cagtcgtacg agtgaacgtg aaggagcatt acggcaccgg tgaggtatgg agcgacgcgg
      241 ggaacgagac tgcggcggcg gaagacgtgc gccagtggtg cgaggagaac cccccgtggg
      301 agatgcgcaa cctggagccg gtgcgcggtg acgacgggcg gtataagttc gctgtcgtgc
      361 gcgcctatgg aagggtgact taagatggtg ctccataaga tagaagagat cgccgacgag
      421 gagagccgcc ttcagcagct ccggtcgctc gtggatcgcg cgcaagagga gatccgtacg
      481 gggaagatcg cttccgagga ggtagagcag gtgatcgccg gtctgcgccg cgacgcggag
      541 cgcctctttc ccggaacggg aaacaccttc gatctgatct accggccgcg cctggagcgc
      601 gctgcccggg aagcccagga gcgggttgcc gtctgacgcc aggcccgagg tccggatacc
      661 gaagaaagga gcggtgggat gggaggagaa cggcgcgtcc cggagaacat agtagtgtat
      721 gtgcgcccga actgctcgga gtgcgagcag gtgatccgct acctggagaa ccgcggcctg
      781 cgctttcggg tgcgcgatgt ggagcgcgat gcggatgccc tgggggatct ggacgctctc
      841 ggctacaccg atgtcccggt caccttcctg gatgaggagg tcgtcgttgg ctttgatacc
      901 aaccgtctgg atgcctccct cgtgaatatc acggtatagc aggcaatgcc cgtaaacccc
      961 gcagacccat gagcgaagtc gatatttcag tcgtcgttcc agttttcaac gaggaagggt
     1021 gtctggcgga gctgcaccgc cggctgaccg ccgtgctgcg gaaggtggca ggcagctatg
     1081 agatggtctt cgtggacgat ggcagccgcg atggctctct cgccttgctg cgcgagctgc
     1141 acgcgggcga ccgcgagcac gtgcgcgtca tctcgctctc gcgcaacttc gggcacgaga
     1201 tggcctcgac ggccggcctg cggtatgccc gggggcgtgc cgtcgtcctc atcgatgcgg
     1261 acctccagga tccgccggag ctgatccccg agctggtggc gcgctggaag gagggatacg
     1321 acctcgtcta tgcccagcgc gcgacgcgag cgcgtgagac gtggctgaag cgggcgacct
     1381 cctttgcctt ctaccgcgtg atgcgccgcc tggcgaacct cgacctgccc cgggataccg
     1441 gcgatttccg gctgatggac cggaaggtgg tggacgcgtt caaccgctgc tccgagcgca
     1501 accgcttcgt gcgcgggctg atctgctggg ttgggttccg gcagacgggc gtgaaattcc
     1561 atcgcgagcc gcgtttcgcc gggcgcacga agtacaacta cttcaaactg acgcgtctgg
     1621 cgatcgatag catcgtcgct ttctccatcg tgccgctgcg cctggcaacc ctcctcggtg
     1681 gggtgatcgg cattgggagc ttcgtgatgt tcctcctcgt gctcctggag aagctgcgcg
     1741 ggttgggtcc ggagcgtggc tatgcccttc tcggcacggg cgtgctcctg cttggcagcg
     1801 tgcagatctt cctcctgggg atcatcggcg agtacatcgg gcggatctat caggagacgc
     1861 agcgccgccc gctcttcctc gtccgcgagg agatcggcta ccaggagagc cccgctgccg
     1921 atccggcgtt ctttatgtga agtttcgagt gaaggccagt cactggcgcg agctgcgccc
     1981 gactgcgcga gcctggcccg cgggaggagg tcactcccgt ttactcgcgc tcttaccttt
     2041 gatacctgga gttacatgct gaagatcact ttcttgggca cgggcacgtc tcatggcgtg
     2101 cccgtgatcg gttgcgattg ccccacgtgc acctccaccg atccgcgcaa ccaccgtacc
     2161 cgctgctcca tcctggtgga acaaaacggc gcctccgtgc tggtagatac atcccccgaa
     2221 ctccggcttc aagcgatccg cgcaggcctc aagcgtgtcg acgccgtcct cttcacccat
     2281 gcgcacgcgg atcatgtctt tggtctggat gatgtccggc gcttcaatga cctgcagggc
     2341 ggagcgcttc cctgctatgg cgatagcacc accctgcgtg agttgcgccg catcttccgc
     2401 tatgcctttg taccgacgca agtgggcggc gggaaacccc gcctggagct gcgccgcctg
     2461 ttgcgcccga gcttcaatct ccacggtctg gaagtgcgcc ccttgcgcgt gatgcacggc
     2521 cagctcccga tcaccgccta ccgctttggc aagtcggcgt atgttaccga cgccagcttc
     2581 cttccggaag agacggtggc gcagttacag ggcctggacc tgctcatcct ggatgcctgc
     2641 gccgccggcc acacgcgacg cacttcaacc
//
LOCUS       DAOSCV010000635         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24886_length_2668_cov_2.714504, whole genome shotgun sequence.
ACCESSION   DAOSCV010000635 DAOSCV010000000
VERSION     DAOSCV010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24886_length_2668_cov_2.714504"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLY56_14295"
     CDS             <1..432
                     /locus_tag="PLY56_14295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22696.1"
                     /translation="SGGYLHLPELIYPYVKNACVFSCPSERTQGQTFDHGIRLTYGYN
                     QSRFQGRTYFFEGNLSLSMVEDTAQTILFIDDVNLYAGPYDPHVPVGYDPNTPVLNSP
                     SDTRGTRAYNRHNEMYNVAFVDGHVKSMKQTLLRHWSYWED"
     gene            complement(526..912)
                     /gene="rplL"
                     /locus_tag="PLY56_14300"
     CDS             complement(526..912)
                     /gene="rplL"
                     /locus_tag="PLY56_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ22697.1"
                     /translation="MATTEELIEQLGNMTVFQLVELSKALQEKFGVSAAMPVAAVGAP
                     AAAGAAEAAPAEEEQTSFDVILASAGDNKIQVIKAIREITNLGLGDARSFVNSAPVKV
                     KEGVSKEEAEAIRAKLEEQGAAVEIK"
     gene            complement(961..1614)
                     /gene="rplJ"
                     /locus_tag="PLY56_14305"
                     /pseudo
     CDS             complement(961..1614)
                     /gene="rplJ"
                     /locus_tag="PLY56_14305"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
     gene            complement(1769..>2668)
                     /locus_tag="PLY56_14310"
     CDS             complement(1769..>2668)
                     /locus_tag="PLY56_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOJ22698.1"
                     /translation="LARDRFLPRQFANVGDRLVYNNGIIALALLSAALLVVFQGSVHG
                     MLPFYAVGVFLSFTISQAGMVVRWRRIRTPGWRRNATINGIGTCTTFVVMVILAVTRF
                     MPPDSNVLFTLPFAIPGIGETIRSGAWLVVVAILVIEWLFGRIRGHYMAVAAQLTLIG
                     FRPEPPKKNTVIVLVPGVNRGIYPAIQYAKSLGQDTRALHIEIDPETTPRLLDEWEEW
                     GEGIPLLILESPYRSLVEPLFRYLDEVQVERDHVVTIIIPEFVPLKWWHKLLHNQSGL
                     LLKVALYFRKDVIVTNVQYQLRE"
BASE COUNT          503 a          895 c          805 g          465 t
ORIGIN      
        1 tccgggggct acctgcacct ccccgagctg atctacccct acgtcaagaa cgcctgcgtg
       61 ttcagttgcc ccagcgagcg gacgcagggc cagacatttg atcacggcat ccggctgacc
      121 tacggttata accagagccg gttccagggg cgcacctact tcttcgaagg caacctgtcg
      181 ctctcgatgg tggaggatac ggcgcagacg atcctcttca tcgatgatgt gaacctctat
      241 gccgggccct acgacccgca tgtgccggtg gggtatgacc ccaacacccc cgtgctcaac
      301 tcccccagcg atacaagagg gacgcgggcc tacaaccggc ataacgagat gtacaacgtc
      361 gccttcgtag atgggcacgt gaagtcgatg aagcagacgc ttctccgcca ttggtcctac
      421 tgggaggatt gagcgcgcgc cggggcagaa cgctctcggc ccgcatacaa acgcgggggc
      481 gcgggctaat gcccgcgccc ccgcaacaca ctccctcgcg gggtgttact tgatctcgac
      541 ggcagcgccc tgctcctcga gcttcgcgcg gatcgcctcg gcctcttcct tcgacacgcc
      601 ttccttgacc ttcacagggg cggaattgac gaagctcctg gcgtcgccca ggcccaggtt
      661 ggtgatctcg cggatcgcct tgatgacctg gatcttattg tcgcccgcgc tcgcgaggat
      721 gacatcgaag gaggtctgct cttcctcagc cggggcagcc tcggccgcac cagcagcggc
      781 aggcgcgcca accgccgcga cgggcatcgc cgcgctgacg ccaaacttct cctgcaacgc
      841 cttcgacagc tcgacgagct ggaagaccgt catgttaccc agttgctcga tcaactcctc
      901 ggtggttgcc atgtcaacct ctccctctgc agattcccct tgctctcgcc gcttgtcggc
      961 gatcgccatc aacgtgaaaa caaaactagc gaccgtgcca ttcagagtgc ccacaaaccc
     1021 ggtgagcggg ctcatgatgg agcccaccac ctgcgcctgc agttgctcgc gcgtcggcat
     1081 gtcggcaagc gcgcggacct ggtcacccgg gtagacccga ccctcgaagt accctccctt
     1141 gagagagaag ttcttatggt cgcgcgtaaa tgccgccacc gccttcgcca ccgcgaccgg
     1201 atcgctgtag gcgaacacgg cagccgtggg gccggtgagg tactcgtcaa ggtgcgcagg
     1261 tgtgtcctgg gttgccaggc gcagcagcgt gttcttgacc actttcagct cgcccccggc
     1321 ctcgcggatc tgccgacgga gggccgtcaa ggccggcatc ggcagcgccc ggaactcggc
     1381 aaggaaggtc gcctgggagg tctcaacctt ttcgcgcagt tgcgctacgg tctcttcctt
     1441 ctgcggattg cgcacggatt caccccctct cgccgccgcc agtccagagc gcttccgcga
     1501 tctggcgtgc cagcggtcgg cttccttcgc cggcccgcag gagccggcaa aacgacaaag
     1561 gcccccgcca tgaagcggag gccttccaac aaggcgcgcc cagaggacgc ccatgcgcat
     1621 gggaaaggtg gactccgacc tcggcaggcg gcgagaagct cgctcttatc cggtaggacc
     1681 tgctgtctgt ggcggaacgg cccgcgggca tgatgcccgt tatgatgaca cgcggattat
     1741 accacctcgg cgcggcggtg tcaaggcatc actccctcag ctgatactgc acgttggtga
     1801 cgatcacgtc cttgcggaag tagagcgcaa ccttcaggag cagccccgat tggttgtgca
     1861 acagcttgtg ccaccatttg agaggtacga actcggggat gatgatcgtc accacatggt
     1921 cgcgctccac ctgcacctcg tccagatagc ggaagagcgg ctcgaccaac gagcggtagg
     1981 gcgactccag gatgagaagt ggaatcccct cgccccactc ttcccattcg tcgagcagtc
     2041 gtggtgtcgt ctccgggtcg atctcgatgt gaagcgcgcg cgtatcctgg cccagtgact
     2101 tcgcgtactg gatcgcggga taaatgcccc ggttgacacc cggcaccagc acgatgacgg
     2161 tgttcttctt cggcggctcc ggccgaaacc cgatgagcgt cagctgcgcg gcaaccgcca
     2221 tgtagtggcc gcgaatccgc ccgaagagcc actcgatgac caggatcgcc accaccacca
     2281 gccacgcgcc cgagcgtatc gtctctccaa tccccggaat tgcgaatgga agcgtgaaga
     2341 gcacgttgga gtccggcggc atgaaccggg tcaccgccag gatcaccatc acgacaaagg
     2401 tcgtgcacgt tccgatgccg ttgattgtcg cgttccggcg ccagccaggc gtccggatgc
     2461 ggcgccagcg gaccaccatc cccgcctgcg agatcgtgaa cgagaggaag acgcccaccg
     2521 cgtagaacgg gagcatgccg tgaacgctcc cctggaagac gaccaggagc gccgccgaaa
     2581 gcaaggcgag cgcgatgatc ccgttgttat agaccaggcg gtcgcccaca ttggcgaact
     2641 gacggggaag gaaccggtcg cgggccaa
//
LOCUS       DAOSCV010000636         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24969_length_2662_cov_2.635213, whole genome shotgun sequence.
ACCESSION   DAOSCV010000636 DAOSCV010000000
VERSION     DAOSCV010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24969_length_2662_cov_2.635213"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..604
                     /locus_tag="PLY56_14315"
     CDS             212..604
                     /locus_tag="PLY56_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22699.1"
                     /translation="MEQERLTRRQADVYRFIRDFAAANRYLPSQRQIADGLGMCPTTA
                     RGHVCELVRKGFLGMHHHETRGMWLAVELTDEQLPKPTGRRPPVELTCPGCGARVKAD
                     PQSREGFQRLARAFQNEHAACSAAAEGE"
     gene            625..837
                     /locus_tag="PLY56_14320"
     CDS             625..837
                     /locus_tag="PLY56_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ22700.1"
                     /translation="MPVSYQSPARAAREAAGLTVEQAARAIGRTPAYLARCELHGMPL
                     RLAERLAKLYTCRLDVFLPRRHRDGN"
     gene            1031..1447
                     /locus_tag="PLY56_14325"
     CDS             1031..1447
                     /locus_tag="PLY56_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22701.1"
                     /translation="MPIEFKCPNCGTPLTAPDTAAGSSGPCPRCKARVTAPASVFTQA
                     AEPALDTSDMSLTIDLGSVTSVCGELHFRLKIEGDGRGHLQIFAFDPDDMHESGILLL
                     LDEGGYGDLKRVLAKADATIQKIRSAGQMRGLILPY"
     gene            complement(1502..2512)
                     /locus_tag="PLY56_14330"
     CDS             complement(1502..2512)
                     /locus_tag="PLY56_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ22702.1"
                     /translation="MAKRQHHFSATEGALSVKECGHLAEGWFADGEMRQLSEYTLVAR
                     RSTLRKLIWFLEQQGAETVGTNEIRAFLSYVANGHKQDGGRWGNTETRAKRAVRPTTV
                     HTYFAILRSFFAFVVEEGALPVSPIAGIKPPVARADQVQPFTPEQVSALLGAAKKSKN
                     GKRDEALLLFLLDTGARASEVCALKREDLDLTNRRCQVIGKGNKRRTIFFGTSTKQAL
                     WRYIGEQKHAAGDPLFIGDRGLCQGEALQRCGLLKLIHRLGSAAGVDAVRCSPHTFRH
                     TFAVEFLRAGGNTFTLQQLLGHTSLAMTNRYVALAQADLEAQHRAFSPADRLRGAKGK
                     GR"
BASE COUNT          459 a          925 c          849 g          429 t
ORIGIN      
        1 tgattcgggg caacttcaaa ccgcccggca tcgggagggc ccaccacaag cccacgcgcc
       61 cattctagcg ccgcccgacg gacgtgccta tccgtgcaga tgcacccctc tgtgtgtttg
      121 cccgtgcaaa cgaacgggtt gcggtacaaa ctcctgtgtg ctagaatccc gtccgaacga
      181 ccgcctgagg agggagcgtg aagagggggc aatggaacag gagagactga cgaggaggca
      241 ggccgacgtt taccgtttca tccgtgactt cgcagccgcg aaccgctatc ttccttcaca
      301 gcgccagatc gccgacgggc tggggatgtg cccgacgacg gcgcgcggcc acgtgtgcga
      361 gcttgtgcgc aagggcttcc tcgggatgca ccaccacgag acgcgcggca tgtggctcgc
      421 cgtggaactg accgacgagc agcttcccaa gccgaccgga cggcgcccgc ccgtcgagtt
      481 gacctgcccc ggatgcggcg cccgcgtgaa ggccgacccc cagagccggg agggcttcca
      541 gcggttggcg cgggcattcc agaacgagca cgccgcctgc agcgcggctg ccgaggggga
      601 gtgagaggac ttcgagagga ggtgatgccc gtgagctacc aatcgccagc gagggcagcg
      661 cgcgaggctg ccgggttgac ggtggaacag gccgcccgtg ccatcgggcg gacgcccgca
      721 tacctcgcgc gctgtgagct gcacggcatg cccttgcgcc tggccgagcg attggcgaag
      781 ctctacactt gccggttgga cgtgttcctg ccgcgccgcc atcgcgacgg gaactgacac
      841 agggcagtgt gatgccgggc cggtaggttg ccgcctgccg gcccttccca cccccggagc
      901 gctacggtgc gccgtgccct cgcaacgcat gccacagcgc tgcaaggact ctttcgccac
      961 ctcgccgaac tcccctggtg caactcgcgc tcgtgctgcg cgccctgcct tcgcacagag
     1021 gaggttccac atgccgattg agttcaagtg cccgaattgc ggaacgccac tcaccgcacc
     1081 cgacaccgcc gccggcagct ccggcccttg ccccagatgc aaggcacgcg tgacagcccc
     1141 cgcctccgtc ttcacccaag ctgccgagcc ggcgcttgac accagcgaca tgtcactgac
     1201 gatcgacctc ggcagcgtga ccagcgtgtg cggtgagctg cacttccgcc tcaagattga
     1261 gggcgacgga cgcggccacc tgcagatctt cgccttcgac cccgacgaca tgcacgagtc
     1321 gggtatcctc ctgctgctag acgaaggcgg ttacggcgac ctgaagcgcg tcctcgccaa
     1381 ggctgacgcc actatccaga agatccgctc tgcgggccag atgcgcggcc tgatcctccc
     1441 atactgagcc gttctgcacc aagggccggg caccgcgatc cccggccctt ggtgcccact
     1501 cctaccgccc cttgcccttg gccccccgaa gccgatccgc cggcgagaac gcccggtgct
     1561 gggcttccag gtcggcttgc gccagcgcca catagcggtt cgtcatcgcc agggaggtat
     1621 gcccgagaag ctgttggagg gtgaaggtgt tcccgccggc ccgcaggaac tcgacggcga
     1681 aggtgtgccg gaaggtgtgc ggagagcagc gcacggcatc gacgcccgcc gccgaaccga
     1741 gccgatggat cagcttcagc agcccgcagc gctgcagcgc ctccccctgg cacaggcccc
     1801 gatccccgat gaagagggga tcccccgccg cgtgcttttg ctccccgatg tagcgccaga
     1861 gcgcctgctt cgtggacgtg ccgaagaaga tcgtgcgccg cttgttcccc ttgccgatca
     1921 cctggcagcg ccggttcgtc aggtccagat cctcccgctt cagcgcgcag acctccgacg
     1981 cccgcgcgcc cgtgtcgaga aggaacagaa gaagggcctc gtcgcgcttg ccgttcttcg
     2041 acttcttcgc cgcgcccagg agggccgaca cctgctccgg cgtgaagggc tgcacctggt
     2101 cggcacgcgc cacaggcggc ttgatgcccg cgataggaga cacgggaagc gcgccttctt
     2161 ccaccacgaa cgcgaagaag gagcggagaa tggcaaagta ggtgtggacc gtggtaggac
     2221 gaacggcgcg cttggcccgg gtctcggtgt tgccccaccg gcccccgtcc tgcttgtggc
     2281 cgttcgcgac gtaggagagg aacgcgcgga tctcgttcgt gccgaccgtc tccgcgccct
     2341 gctgttcgag gaaccagatc agcttgcgga gcgtggaccg gcgcgcgacc agcgtatact
     2401 ccgaaagctg gcgcatctcg ccatcggcga accagccttc ggccaggtgg ccgcattctt
     2461 tgaccgagag ggcgccctcg gtagcgctga aatgatgctg ccttttcgcc acttttgccc
     2521 tccagaaccg ccattctgaa gggcagtgtg atgccagaaa cccttgcgct atctagcttt
     2581 tcaagaaact ggagcgggag acgagattcg aactcgcgac cttctccttg gcaaggagac
     2641 gtactaccac tgtactactc cc
//
LOCUS       DAOSCV010000637         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_24995_length_2660_cov_1.789251, whole genome shotgun sequence.
ACCESSION   DAOSCV010000637 DAOSCV010000000
VERSION     DAOSCV010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24995_length_2660_cov_1.789251"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLY56_14335"
     CDS             complement(<1..445)
                     /locus_tag="PLY56_14335"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ22703.1"
                     /translation="MAVRQESPEEAGFDGKRLERLAALLEQAVAAGETPGAVALVADR
                     QSVVFHRAFGHAQVVPRAEPMRPDTLFDLASLTKVVATAPAIRLLVEAGAIRLQDRVV
                     EFIPEFAGEGKDEVDLRHLLTHCSGLPAWKPFYEQGASPEELFATI"
     gene            complement(565..894)
                     /locus_tag="PLY56_14340"
     CDS             complement(565..894)
                     /locus_tag="PLY56_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22704.1"
                     /translation="MNPSRPTIHCRVFSLEPPRCEFAPDLPEYSDAVVTFDFTDALLT
                     DLGPTLLMLYASYAGPPITRDALVELAQPFVAIEVEHATQADLIARAREEYPAVLARA
                     RERIAAL"
     gene            complement(894..1823)
                     /locus_tag="PLY56_14345"
     CDS             complement(894..1823)
                     /locus_tag="PLY56_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ22705.1"
                     /translation="MERLSQLLQVDPRQETAFSPGHNACPGCGSTTALRMVLNAVGDR
                     AIYFVPASCASLYFTPSDSTTTRVPVIHTLFPGAFAEAEGMARALQLQGRDEKVVVWA
                     GDGCCYDIALGSLSGAAARGANLLVICNDNEGYQNTGGHLSSATPPDAQTRTGRRGDQ
                     VPGITRRKDLVEIMAAHRVPYVATASVAYPEDLQSKVLKAREMEGFRMLLLLTPCVTW
                     GFESRYSVKLARLAVETGYFPLYEVEGGRHYRITHEPAMLPLERFTRLQRRFNGGDLN
                     ALHEEIDEKWAELRFRSERSRRERVQRPAWVAA"
     gene            complement(2008..>2660)
                     /locus_tag="PLY56_14350"
     CDS             complement(2008..>2660)
                     /locus_tag="PLY56_14350"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22706.1"
                     /translation="ETEEVLIPDEKAAAAFVGERMRCEAPMPDPQADYGGLMPSPKYF
                     RAYQRMHHDVRRAAAVFDSAAQEFATRFGRHYGRISPLYTEDAETVVVSAGTICGTIR
                     EVVEQRRERGECVGMVKLHAFRPFPAEALREALGNARRVIVIDRNLAPGLGGIFASEI
                     RLALHARGVPATVYSFIAGLGGSDVPPGLIESALDYAGEQADPPDTLFLLDTGIEA"
BASE COUNT          450 a          867 c          895 g          448 t
ORIGIN      
        1 agatcgtggc gaacagctcc tcggggctag ctccctgttc atagaagggt ttccaggcgg
       61 gcagcccgga gcagtgcgtc aggaggtggc gcaggtcgac ctcatccttg ccctcgcccg
      121 cgaactcggg gatgaactcc acgacacggt cttgcaggcg tatggcgccc gcctcgacca
      181 gcagccggat cgctggggct gtcgccacca ccttcgtcag cgatgccagg tcgaagaggg
      241 tatcggggcg catcggctcg gcgcgcggaa ccacctgcgc gtgcccaaag gctcggtgga
      301 agacgacgct ctgtcggtcc gcgaccaggg ctacggcacc cggtgtctcg cctgcggcaa
      361 ccgcctgttc gagaagcgcc gcgaggcgct ccaggcgttt cccatcaaac cccgcctctt
      421 caggagactc ctgtctcact gccatcccct atcttttcca ggatgcctcg cctgccggat
      481 gacccactgc gagccattgc gtggggcctg cgcaactacg gcatgggatc aattcgcgac
      541 agccagtggg tttccttctg ggggttagag cgcggcgatc cgctcgcggg cgcgcgccag
      601 cacggcggga tactcttcgc gtgcccgtgc tatcagatcc gcctgagtcg catgctccac
      661 ctcgatggcg acgaacggct gcgcaagctc gaccagggcg tcgcgcgtaa tcggcgggcc
      721 agcataggac gcatatagca tcaggagcgt tggaccgaga tccgtcagga gggcatccgt
      781 gaaatcgaac gtcacgaccg catcggagta ctccggaagg tccggggcaa actcgcaccg
      841 cggcggttcg agggagaaga cgcggcagtg gatggtgggg cgagagggat tcatcaggct
      901 gccacccacg cgggccgttg cacgcgctcg cgccgggagc gctcgcttcg gaagcggagt
      961 tcggcccact tctcgtcgat ctcttcgtgg agcgcgttga ggtcgccccc gttgaagcgt
     1021 cgctggaggc gcgtgaaacg ttccaggggt agcatggccg gctcgtgcgt gatccggtag
     1081 tggcgcccgc cctccacctc atagagcggg aagtagcccg tctctacggc caggcgcgcc
     1141 agcttcaccg aataccggct ctcgaatccc caggtgacgc acggggtcag gagcagaagc
     1201 atgcggaagc cttccatctc tcgcgctttg agcaccttcg attggaggtc ttccggatag
     1261 gccacggaag ccgtcgcgac gtagggcacg cggtgcgccg ccatgatctc caccaggtcc
     1321 ttgcgccgcg tgattccagg aacctgatcg ccgcgccggc ctgtgcgcgt ctgcgcatcc
     1381 gggggtgtcg cggatgagag gtggcctccc gtgttctggt agccttcgtt gtcgttgcag
     1441 atgaccagga gattggcacc gcgcgccgcg gcgccggaga gcgagcccag ggcgatgtcg
     1501 taacaacagc cgtccccagc ccatacgacc actttctcgt cgcggccctg gagctggagt
     1561 gcacgcgcca tgccctcggc ttccgcgaag gcgcccggga aaagagtgtg gatcactgga
     1621 acgcgagtcg tggtggagtc cgaaggagtg aaatagaggc tggcgcagct cgcgggcacg
     1681 aagtagatgg cgcgatcgcc cacggcgttc aacaccatcc gtaacgcggt tgtggagcca
     1741 caccctggac acgcattgtg cccgggtgag aacgccgtct cctgccgcgg atccacctgc
     1801 aacaactggc tcagtctttc catccgccgc ttcccccggg gcgctggcat cgcaaactgc
     1861 tcctccccgc aagggagtgt tgccagcacg cctgctaagc gatttagagg gacacctgat
     1921 cgcgaatgtt tcgctctctc cgcgtgcaaa gcaagatcgg atggcgtatt gttcgcagat
     1981 tctccttcta gccggtcccc agaaggctca ggcctcgatc ccggtgtcaa ggaggaagag
     2041 cgtgtccggg ggatcggcct gctcgccggc gtaatccagg gcgctttcga tcaacccggg
     2101 ggggacgtcg gacccgccca ggccggcgat gaaggaataa accgtcgcgg gcacgccgcg
     2161 ggcatggagc gccagccgga tttcggatgc gaagatcccg cccaggccag gggccaggtt
     2221 gcggtcgatc acgatgacgc ggcgggcgtt ccccagggcc tcccgtagcg cctcggcggg
     2281 gaagggccga aacgcatgca gcttgaccat gccgacgcac tcgcctcgct cgcgccgttg
     2341 ctccacgact tcgcggatgg tgccgcagat agttcccgcc gagaccacca ccgtctcggc
     2401 atcctccgtg tagaggggtg agatccggcc atagtggcgg ccgaagcgcg tggcgaactc
     2461 ctgcgcggcc gaatcgaaga cggcggcggc gcggcgcacg tcgtggtgca tgcgctggta
     2521 ggcgcggaag tacttgggag agggcatcag cccgccatag tcggcctgcg ggtcgggcat
     2581 cggggcctcg cagcgcatcc gctcacccac aaacgccgcg gcggccttct cgtcgggaat
     2641 gaggacctcc tcggtttcgt
//
LOCUS       DAOSCV010000638         2659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25008_length_2659_cov_2.586790, whole genome shotgun sequence.
ACCESSION   DAOSCV010000638 DAOSCV010000000
VERSION     DAOSCV010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25008_length_2659_cov_2.586790"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PLY56_14355"
     CDS             complement(<1..2048)
                     /locus_tag="PLY56_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22707.1"
                     /translation="MKALFVSIALLLGIGTTQAAPLLYDMGTEKSRVWEGFTPVTTQS
                     VYTETAGYGWQTAEGLKALVRAYTEPVENQGRGTTEHPPIWTNPITEDTHVGDRENAF
                     LVKATPGEVEVYVVCGSSERTRGQYYDFTVSVGGQEQRVQIEAGQYFRSLRFRAKATA
                     EPLAIRFSPRSKFLVNAIMVWSPADSARVEKEIIKPFEEWTYRLPPEEWAKWQELPGP
                     EPGPMPPVSAADEKRGFLVWSRHYLECIFPNTRPRPEELNPELRLFATPGEYEPATFT
                     VHPLRDLSDAKVTVTALGPIPAARVDVRRVRYMLARPNYRVTYHYQVVPDILERFDRL
                     DLKAGENARFWLTVHVPENTPAGTYTGKAIFECSGGKAEVPIRIRVLPFQLQEDETKI
                     YGIYYRHPYDEMARATDDVSREYYRRKADLEHRDMVAHGTRNVVLSVPSTAADAEGNF
                     KVDWDLMAAKIELWRKHGFRGPIVTSISCGAVYYKYMKESPGSHLHAVKDPPPEFARE
                     LTALVKYIEGERRKRGWPEFLYYPIDEPGTHPASVRFMVTVLKACKEAGVRTYVTADP
                     THEQFAPMRPYVDVWCTQPFAPDRQTILADMKARNVEYWCYPNHVNGENNHTPVAGAR
                     MTYGFGFWRSGFRTLIPWIYSSTTGDPFNYLDGPSMDFFNRSEPDGTPIPVAMWEAYR
                     "
     gene            complement(2218..>2659)
                     /locus_tag="PLY56_14360"
     CDS             complement(2218..>2659)
                     /locus_tag="PLY56_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22708.1"
                     /translation="VKIPHSHIPDHHDVRFIQFNIAESNYNHGDRVDFYIDDLALLRY
                     ARPTLLEFAAESGVIFADTRQLPVRFRMAGVKPGETATVTCELRGNRGVVARETAKVE
                     HGAQRLVLDLGTKGLAPGTYILSARTSDGAPVTARVRVVESPWR"
BASE COUNT          456 a          845 c          884 g          474 t
ORIGIN      
        1 tcgcggtagg cctcccacat cgccaccgga atcggcgtgc cgtccggctc gctccgattg
       61 aagaagtcca tcgacggccc atccaggtag ttgaacgggt cgccggtggt tgaagaatag
      121 atccatggga tcagcgtgcg gaaaccggag cgccagaagc caaagccata ggtcatgcgc
      181 gcgccggcga cgggggtgtg gttgttctcg ccgttcacgt ggttgggata gcaccaatac
      241 tccacgttgc gcgccttcat atcggccagg atcgtctgcc ggtcgggggc aaacggctgg
      301 gtacaccaga cgtcgacgta ggggcgcatc ggcgcgaact gctcgtgcgt cgggtcggcg
      361 gtcacgtagg tgcgcacccc cgcctctttg cacgccttga ggacggtgac catgaagcgg
      421 accgaggccg ggtgggtgcc aggctcatca atcgggtaat agaggaactc gggccagccg
      481 cgcttgcgac gctctccctc gatatacttc accagagctg tcagctcgcg ggcaaactcc
      541 ggcggaggat ccttcacggc atgcaggtga ctaccgggcg actccttcat gtacttatag
      601 tagacggcgc cgcagctgat gctggtcacg atcggcccac ggaagccgtg cttgcgccac
      661 agctcgatct tggccgccat caagtcccag tccaccttga agttgccctc ggcatcggcg
      721 gccgtactgg ggacggagag gacgacgttg cgcgtgccgt gcgcgaccat gtcgcggtgc
      781 tccagatcgg ccttccggcg gtagtactcg cgtgagacgt catcggtggc acgcgccatc
      841 tcgtcgtagg ggtgacggta gtagatgccg tagatcttcg tctcgtcttc ctgaagctgg
      901 aagggaagca cgcggatgcg aatcggcacc tcggccttcc cgccggaaca ctcgaagatc
      961 gccttgccgg tgtaggtgcc cgccggggtg ttctccggca cgtgaaccgt cagccagaag
     1021 cgcgcgttct caccggcctt cagatcgagc cggtcgaagc gctccaggat atccggaaca
     1081 acctgatagt ggtaggtgac ccggtagttc ggacgcgcga gcatgtagcg gacccggcgc
     1141 acatccacgc gcgctgcggg aatggggccg agcgccgtga ccgtcacctt cgcatcagaa
     1201 aggtcgcgca aggggtgcac cgtgaaggtc gccggctcat actctcccgg ggtggcgaag
     1261 aggcgcagtt ccgggttcag ttcctcgggg cgaggccgcg tgttggggaa gatgcactcc
     1321 aggtagtggc gtgaccagac caggaagccg cgtttctcat ccgcggcgct caccggcggc
     1381 atcgggccag gttcgggccc gggaagctcc tgccacttcg cccactcctc ggggggcagc
     1441 cgataggtcc actcctcgaa cggcttgatg atctccttct ccacgcgtgc ggagtccgcc
     1501 ggggaccaga ccatgatagc gttgaccagg aacttgctgc gcggggagaa gcggatcgcc
     1561 agtggctccg cggttgcctt cgcacggaag cggagcgagc ggaagtattg gccagcctcg
     1621 atctgaaccc gctgctcctg cccgccgacg ctcacggtga agtcgtagta ctgcccgcgc
     1681 gttcgctcgc tgctcccgca aacgacgtag acctcgacct ccccgggcgt tgccttcacc
     1741 aggaaggcat tctctcggtc gccgacatgg gtatcctcgg tgatcggatt ggtccagata
     1801 ggcggatgct cagttgtgcc ccggccttgg ttctccaccg gttcagtgta ggcgcgcacg
     1861 agggccttca gcccctccgc cgtctgccag ccatagccgg cggtctcggt gtaaaccgac
     1921 tgcgtggtga caggcgtgaa cccctcccac acgcgcgact tctccgtgcc catatcatac
     1981 aggagaggcg cggcctgggt ggttccaatg cccaacagca gcgcgatgct aacgaagagt
     2041 gctttcatga gtagctcccc atcgagcgaa actcggcctg gcgccaggcg tgacctcggc
     2101 gaccgggaga cccacccagc aggctggcga gggcctcctg cacggcgaga tcctgattgg
     2161 gcgcgtgccg ctccccgatc cgcgagggcc gcgagtctct gcatcgaagc cgttccctta
     2221 ccgccacggc gattctacga cgcgcacgcg cgccgtgacc ggagcgccgt cagaagtgcg
     2281 tgccgagagg atgtaggtgc cgggggcgag ccccttcgtg cccaggtcta gcacgaggcg
     2341 ctgcgcgcca tgctccacct tcgcggtttc acgcgcgaca acgccacgat tgccgcgcag
     2401 ttcgcacgtg acggtggcgg tttcgccggg cttcacccct gccatccgga agcggaccgg
     2461 taactggcgg gtatcggcaa agatcacccc gctctcggcg gcgaactcca ggagcgttgg
     2521 ccgggcatag cggaggagcg ccaggtcatc aatgtagaaa tcgacccggt cgccgtggtt
     2581 atagttggac tccgcgatgt tgaactgaat gaagcgcacg tcgtggtggt cggggatgtg
     2641 cgagtggggg atcttgacc
//
LOCUS       DAOSCV010000639         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25032_length_2657_cov_2.820907, whole genome shotgun sequence.
ACCESSION   DAOSCV010000639 DAOSCV010000000
VERSION     DAOSCV010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25032_length_2657_cov_2.820907"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLY56_14365"
     CDS             complement(<1..998)
                     /locus_tag="PLY56_14365"
                     /inference="COORDINATES: protein motif:HMM:NF022824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HOJ22709.1"
                     /translation="MASEAELIELRYHVASLVAVFLALAVGILIGSSMVGTPSVESQI
                     RRLKSGFDRIQAEDRRLRAENEQLRGQARLLENALRQTVPAVVQGRLVGKRIGLLITG
                     QGIDTSLVRETKYLLTTAGASVGSITTLQEPLLPENPNSRQTILTRSGIDTQDSTRAE
                     RQLAARITRAIVRGNEPDLLRVIGEFSPGLVLDGDYTLPIDAVTLIVGDATEAQAQAM
                     SDGTSIQARIADVLREEGIAAVACERAECPLSLMAYFARYSLTTVDSLDTAVGKIALV
                     LALAGKQGHYGTKPSAAQVLPDLDLEPMPKGESPARSLPRAPQEPGQPRESLLLAPG"
     gene            complement(1081..2259)
                     /gene="steA"
                     /locus_tag="PLY56_14370"
     CDS             complement(1081..2259)
                     /gene="steA"
                     /locus_tag="PLY56_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HOJ22710.1"
                     /translation="MRLPLARAIRGAARPGRKTKKLIPLLHRGDIAIIDHPDIDIVCA
                     NALIDRRVSAVINCAVSLTGRYPAGGPGAILAAGIPLFDSADPDLLERVAPGTVIEIR
                     EDGGIYEGGQRIAAARPFTRALLDEALTRARANISVEMEAFVRNTLDYLSEEWTLLFD
                     EQALPELRTRIHGRDVLIVVRGEGYREDLQAILPYVRDKRPVLVGVDGGADALLEMRL
                     RPDIILGDMDSVSDEALRCGAEVIVHGYPRKPGSTEPEDETAHAPGLARTRKLGVPAS
                     VLRALGTSEDVAMILMERKGARLIVLVGSHFSLQDFLDKGRKGMSSTFLSRLKVGSIL
                     VDAKGVSRLYHRGWRILDIVWLLVGAVTLTLVILSVSDSARAFIDLLAVRLQLWLRHL
                     "
     gene            complement(2259..>2657)
                     /locus_tag="PLY56_14375"
     CDS             complement(2259..>2657)
                     /locus_tag="PLY56_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012686041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ22711.1"
                     /translation="RIASGGELSRVMLALKSILARVDRVPTLIFDEVDVGIGGRTAHV
                     IGEKLAALGKEKQVLCVTHLPQVASRGDLHFTVRKEIQGERTVIRLCRLTDEERVDEL
                     TRMLGGQEGSAMAREHAREMLSRSSGGAER"
BASE COUNT          463 a          884 c          870 g          440 t
ORIGIN      
        1 ccgggcgcga gaagcagcga ctcgcgcggc tgcccgggtt cctggggtgc acgcggtagt
       61 gaacgagctg gagattcacc cttaggcatc ggctccagat cgagatcggg caggacctgg
      121 gcggccgaag gcttcgtgcc atagtggccc tgctttcccg ccagtgccag caccagggcg
      181 atcttcccaa cggcggtatc gaggctatcg acggtcgtca ggctgtagcg agcgaagtac
      241 gccatcagcg agagggggca ctccgcacgc tcgcacgcca ccgcggcaat cccctcttca
      301 cgaagcacgt ccgcgatccg cgcctgaatg gatgtgccgt cggacatcgc ctgagcctgc
      361 gcctccgtgg catcgcccac gatcagcgtg acggcatcga tagggagcgt gtaatcgcca
      421 tccaggacga ggccgggcga gaactcaccg atcactcgca gcagatccgg ctcgttgccc
      481 cgcacgatgg cgcgcgtgat ccgtgcggcc agttgtctct ccgcgcgcgt ggagtcttga
      541 gtatctattc cgctgcgcgt caggatagtc tggcggctgt tggggttctc gggaagcagc
      601 ggctcctgca aggtggtgat tgagccgacc gaggcgcccg ccgtggtgag caagtacttt
      661 gtctctcgca caaggctggt atcgatcccc tggccggtga tgagcaggcc gatccgcttt
      721 cccacgaggc gcccctgcac taccgcgggc acggtctgcc gcaaggcgtt ctccaggagg
      781 cgtgcctgcc cgcgcagctg ctcgttctcg gcacgcaggc ggcgatcctc cgcctggatc
      841 cgatcgaagc cgctcttcag gcgccggatc tgactctcca ccgagggtgt gcccaccatc
      901 gacgagccga tcaggatccc cacggccagc gccaggaaga ccgccacgag actcgctacg
      961 tgatacctta gctcgatcaa ctccgcctca cttgccactc actgcagact catctatcac
     1021 ctagttcgcc gccgcgcagg ccactccctg gcttgagata ggctcccgaa gcacgggcgc
     1081 ctagagatgg cgcagccaga gctggaggcg gacagccagg agatcgatga aggcacgcgc
     1141 gctatcgctt acggagagga tgacgagggt gagggtgacg gcgccgacga gcagccagac
     1201 gatatccagg atgcgccagc cgcggtggta aaggcggctg acgcccttgg catcgacgag
     1261 gatggagccc accttcaggc gcgagaggaa ggtgctgctc atccccttgc gccccttgtc
     1321 gaggaagtct tgcagcgaga agtgagaccc aaccaggacg atcaggcgcg cgcccttgcg
     1381 ctccatgagg atcatcgcga catcctcgct ggtgccgagg gcccggagca ccgaggcagg
     1441 gactcccagc ttgcgcgttc tggccaggcc cggggcatgc gcggtctcat cctccggttc
     1501 cgtcgagccc ggcttgcgcg gatagccgtg gacgatcacc tccgcgccac agcgcagggc
     1561 ttcgtcactc acggaatcca tgtcgccgag aatgatgtcg gggcgcaggc gcatctccag
     1621 gagcgcgtcg gcaccgccat ccacgcccac gagaaccggg cgtttgtcac ggacgtaggg
     1681 gaggatcgct tggagatcct cgcggtagcc ctcgcctcgg accacgatga ggacatcgcg
     1741 tccgtggatg cgtgttcgaa gctcggggag tgcctgctca tcaaagagaa gcgtccactc
     1801 ctcggagagg tagtcgaggg tgttgcggac gaacgcctcc atctcgacgg agatgttggc
     1861 gcgcgcgcgc gtcagggctt catccaggag cgcacgagtg aacggacggg ctgcggcaat
     1921 gcgctggccg ccctcataga ttcccccatc ctcgcggatc tcgatcaccg tgccgggggc
     1981 cacgcgctcc agtagatccg ggtcggcgct gtcgaatagg gggatacccg ccgccaggat
     2041 cgcgcccggg ccgcccgccg gataccgccc agtgagcgag acggcgcagt tgatgacggc
     2101 ggaaacccgc cggtcgatga gtgcgttggc gcagacgata tcgatatcgg ggtgatcgat
     2161 aatcgcgata tcgccgcggt gcaagagggg tatgagcttt ttcgtttttc gtccggggcg
     2221 ggcagcgccc cgaatggctc gtgccagggg aagccgcatc agcgctccgc tcccccggac
     2281 gagcgcgaga gcatctctcg cgcgtgttct ctggccatcg ccgagccctc ctgaccgccc
     2341 agcatccgcg tcagctcgtc cacacgttcc tcatccgtga ggcggcagag gcggatgacc
     2401 gtgcgctccc cctggatctc cttgcggacc gtgaaatgga gatcgccccg gctggctacc
     2461 tggggcaggt gggtgacgca gaggacctgc ttctccttcc ccaaagccgc cagcttctcc
     2521 ccaatgacgt gcgcagtgcg cccgccgatc cccacatcca cctcgtcgaa aatgagggtc
     2581 ggcacgcgat ccacccgcgc caggatcgac ttgagcgcga gcatgacgcg cgacaactcg
     2641 cctccagagg cgattcg
//
LOCUS       DAOSCV010000640         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25075_length_2653_cov_4.068129, whole genome shotgun sequence.
ACCESSION   DAOSCV010000640 DAOSCV010000000
VERSION     DAOSCV010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25075_length_2653_cov_4.068129"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLY56_14380"
     CDS             complement(<1..442)
                     /locus_tag="PLY56_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22712.1"
                     /translation="MQKPSSGSWEFSLSREQAKWFYIGLWGINAFFLVTNVAMAAGWH
                     PPLNTIARLLDMDKPGSLGKWWLSAQLIVVSLGAVLLAGRGAGGGSRIVRWGWPCLAL
                     VTLIMSADSVARMRQDIEGAVRNLLPAGKLMGMELNWTEVFAPAI"
     gene            complement(531..2189)
                     /locus_tag="PLY56_14385"
     CDS             complement(531..2189)
                     /locus_tag="PLY56_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HOJ22713.1"
                     /translation="MSDGMSYARLFRVLLLLMVPVAAVSLIRHAGAESSASIVVDQAG
                     YAPDERKLAFVTGATGRFSVVDPRGRVVFSGALEPAGHAVDAGKNFTRADFTPLKTPG
                     VYMIRLPGGARSPRFRIDSRAHDEVMQVAIRAFRAQECGTAVRLGLPQDHPACHLEDG
                     TLWEERERRIPSVGGWHDAGDYGKYTVNGAYAAGLLLTLAERRQGDLARSLLEVTRCE
                     LEWLLTMQARDGAAYHKLTAVKHPGVLPPEGDRSQRYLFPVSSTATGDLAAAMAIAAR
                     AYAPVDAGFSRRCLEAAKGAWRWLEAHPEPLLFRNPQGVNTGEYGDKDDRDERLWAAV
                     EIYRTTGDEAALRRFRELAEPRRPAFTYAGSWGDVANVALLSFLQEKRVDEGDPLRAR
                     IQRDLISFGRSLAARCARHPLGVVLTPKDYYWGSNGIALTYALLLLEAGRHARDGALI
                     QAGRDQVHYVLGRNALGISFVTGVGERCVRNPHHAPSILTRGQPALPGLLVGGPNHRQ
                     AKPLSEFPATAYVDDPNNWTVNEPAINLNAELVLALGLMDSWEK"
BASE COUNT          486 a          915 c          840 g          412 t
ORIGIN      
        1 ggatcgcagg agcgaagacc tccgtccagt tcagctccat ccccatcagc ttgcccgcag
       61 gcagcaggtt gcgaaccgcg ccctcaatat cctggcgcat ccgcgccacg ctatccgccg
      121 acatgatcag cgtgaccagc gcgaggcagg gccaccccca gcggacgatc cggcttccgc
      181 cccctgcgcc tctgccggcc aggagaaccg ccccgaggct cacgacgatg agctgggcgc
      241 tcagccacca cttgccaagc gagccgggct tatccatatc caacaggcgc gcgatcgtgt
      301 tcagcggcgg gtgccacccg gccgccatcg ccacgttcgt caccagaaag aaggcgttga
      361 taccccacag gccgatatag aaccactttg cctgctcgcg agagagcgag aactcccacg
      421 agccagagga tggtttttgc acggtattcc ctccgttccc cgcccacgcg ggccagtgct
      481 ggcgcctgca cgcactgtgc cgaaaccgcg cgggctccgt agcggagcgc tcacttctcc
      541 caggaatcca tcaagcccag cgcgagcacc agctccgcgt tcaggttgat cgctggctcg
      601 ttcacggtcc agttgttggg atcatccacg taggcggtcg ccgggaactc agagagcggc
      661 ttcgcctgcc ggtgattagg cccccccacc agcaaccccg gaagtgctgg ctggccccgc
      721 gtcaggatcg acggagcatg gtgcggattg cgaacgcacc gctcgcccac gcctgtgacg
      781 aaggagattc ccagggcatt gcgaccaagg acataatgca cctggtcgcg ccccgcctgt
      841 atcagcgcgc catcccgagc gtgccttccc gcttccaaga gaaggagcgc gtaagtgagt
      901 gcaataccgt tggagcccca gtaataatcc ttgggggtga gcactactcc gagcgggtgt
      961 cgggcacacc gggcggccag gctccgaccg aaagagatca ggtcgcgctg gatccgggcg
     1021 cggagcgggt cgccctcatc cacccgcttc tcctgcagga aggaaaggag ggcgacgttg
     1081 gcgacatcgc cccacgagcc cgcataagta aacgcgggcc ggcgcggctc cgcaagctcc
     1141 cggaagcgtc gcaacgcggc ctcgtctccc gtggtccggt agatctccac cgccgcccaa
     1201 agccgctcgt cgcggtcgtc cttatcgccg tactccccgg tgttcacgcc ttgggggttg
     1261 cggaagagaa gcggctccgg gtgcgcttcc agccatcgcc aggcgccctt cgcagcctcg
     1321 aggcagcgcc tggagaagcc cgcgtccacc ggcgcgtacg cgcgcgccgc tatcgccatg
     1381 gcagccgcca gatcgcctgt cgcggtcgaa gagacgggga agagatagcg ctgggagcga
     1441 tcaccttccg gcgggagaac gcccggatgc tttacggccg tcagcttatg gtacgcggcg
     1501 ccgtctcgcg cctgcatcgt gagcagccac tccagctcgc aacgcgtgac ctccaggagg
     1561 ctgcgcgcca gatcgccctg gcggcgctcg gcgagagtga ggagaaggcc ggcggcatag
     1621 gcaccgttca ccgtgtactt gccatagtcg cccgcatcat gccagccgcc cacggagggg
     1681 atccgacgct cccgctcctc ccagagcgtg ccatcctcca aatggcacgc aggatggtcc
     1741 tggggcagcc ccagacgcac cgcagtgccg cactcctggg cgcggaacgc gcgaatcgcg
     1801 acctgcatca cttcatcatg agcccgagaa tcgatgcgaa accgcggcga gcgagcgcct
     1861 ccgggaagcc gaatcatgta gactcccggc gtcttcagcg gtgtgaaatc agcgcgtgta
     1921 aaattcttcc cggcatcgac ggcatgcccc gccggctcga gggcaccgga gaagaccacg
     1981 cgcccgcggg gatccacgac gctgaaccgc ccggtggcac cggtcacgaa ggcgagcttc
     2041 cgctcgtcgg gagcatagcc cgcttgatcc accacgatgc tcgcggacga ctccgcgccg
     2101 gcgtgccgga tcagcgacac ggcggcaacc ggcaccatca gaagcagcaa cacgcgaaac
     2161 agcctggcgt agctcatgcc gtccgacacg gcaagatgcc tgccatccgc gcagggcgat
     2221 cgttgtgtga tccgcggatg ctccagatga aaggggttgg aaagagcaat cgtcttcgcg
     2281 tgatgcggga ggcaggaggg cttcccctga cgcagccgac tcaccaagtg gggacacgta
     2341 tggttcggac gaacctttcg tgttcgatgc acttccggaa caggcgccac ggcttcctgg
     2401 cacctttctt gcggacgcgc ggctcgcaag tcgccagggg ccgcggtttc acgccatcgg
     2461 cggcgcggcg ttctatggga gggatggtat tttggatgcc aacatgagtg acgatcgaca
     2521 attcggcccg tcaggcgacc ggtgccaggt tgaacgcgtt ctctcggtgg agcatctgga
     2581 gagcttggcc gacgcctgga atgccctggc ccagcgtgac ccccaggcgc gcatcttcca
     2641 gagtcacacc tgg
//
LOCUS       DAOSCV010000641         2648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25151_length_2648_cov_3.878905, whole genome shotgun sequence.
ACCESSION   DAOSCV010000641 DAOSCV010000000
VERSION     DAOSCV010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25151_length_2648_cov_3.878905"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            400..1671
                     /locus_tag="PLY56_14390"
     CDS             400..1671
                     /locus_tag="PLY56_14390"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOJ22714.1"
                     /translation="MIPKLETQTIERDYAAAKEIYAAYGVDTDAALQKLSRIHLSLHC
                     WQGDDVGGFENPDGELSGGLAATGNYPGKARTPDELRMDLKKVFSLIPGKHRLSLHAI
                     YVDYPEPVERNALETKHFQRWIDWAIQEGIALDFNGTFFSHPLAADGYTLAHPDPGIR
                     RFWVEHGIASRHIAADMGRAQGSPCINNTWIPDGEKDLPADRWAPRQRLVESLDAMYA
                     EELPAELTRDAIESKLFGIGSESYVVGSLEFYLSYAVSRKKILTLDMGHFHPTEEVSD
                     KISSVLTFIDEVLFHVSRGVRWDSDHVVTLSDPVRAVAEESVRGNVLDRVHFGLDYFD
                     ASINRIAAWVIGARSLLKALLIALVEPTQLLKEMELNGDRTGRLALMEELKMLPFAAV
                     WNRYCEMQGVPVGAAWLNEVRAYERSILSER"
     gene            complement(1789..>2648)
                     /locus_tag="PLY56_14395"
     CDS             complement(1789..>2648)
                     /locus_tag="PLY56_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOJ22715.1"
                     /translation="LLILGVSSLLAPICLSQELVRRDVPLLAVVSLALFLLSVDGRLQ
                     RYEGALLILGLIAYTRHAIRRGRESGEEAGYPAALTEVQVAGHDGWLRHIALVVLGLL
                     LLAAGSALVVNAAVSFARAFGVSELVISLTIVAIGTTLPELATSALAARRGDPDITVG
                     NVIGSSLFNIMGVLGTAALLSPGGLAVPLEAVRLDMPIMITAAVALLPVFVIGAQLER
                     WEGAFFLGYYVTFVAYLYLRGTESQALEGYTRAMAGFVIPLTAVTLLALIAARRQARD
                     QRARSGGDG"
BASE COUNT          514 a          885 c          812 g          437 t
ORIGIN      
        1 aggacatcgg cttcctcgcg aaggatcttc atgcttgctg tcactcccat tccagaccaa
       61 cgccgtcgag gcctaacccg aagtattcac cagaatactt caagaaaaga cttttacttg
      121 gtggcacgcc gttgccgcta caggcatccg gaagcagagt gttttcgggg cagtgcccac
      181 ggacctgggc cacgtctgac agaaagactt cttttcatta ttctgatgga taatgaaggc
      241 taaggcccca agagcgctcc ggtctgccgc gcggttcgat aggtctctgc cgccagggtt
      301 cgccctaggg aaagtatttc ctgccgggct tcccgggcga agggtatcgc gcctagccgt
      361 cgaatgacac ggtaaccgag tctggacgga ggttcgcgca tgataccgaa gctggagacg
      421 cagacgatag aacgagacta cgcggcggca aaggagatat acgccgccta cggggtggat
      481 accgacgcgg cactccagaa attgagccgg attcaccttt cgcttcactg ctggcaggga
      541 gacgacgtcg gcggtttcga gaatcccgat ggcgagcttt cgggcgggct cgcagccacg
      601 ggcaactacc ctgggaaggc acgaacgccc gacgaactgc gcatggacct gaagaaggtt
      661 ttcagcctca tcccgggcaa gcaccggctc tcgctccacg caatctacgt ggactatccg
      721 gagcccgtgg agcgcaacgc cctggagacg aagcacttcc agcgctggat cgattgggct
      781 attcaggagg gcatcgccct cgatttcaac ggcaccttct tctcgcaccc gctggcggcc
      841 gatggctaca cgctcgccca tccggatccg gggatccgcc gcttctgggt ggagcacggt
      901 atcgcatccc ggcacatcgc cgctgatatg ggccgcgccc aggggagccc ttgcatcaac
      961 aacacctgga tccccgacgg cgagaaggat cttcctgcgg accgctgggc gccgcgccag
     1021 cgcctggtgg agtcgctcga cgcgatgtat gccgaggagc tgccggccga actcacgcgc
     1081 gacgccatcg aatccaagct gtttggcatc ggctccgagt cctacgtggt cggctcgctg
     1141 gagttctacc tgtcatatgc cgtcagtcgg aagaagatcc tcaccctcga tatgggccac
     1201 ttccacccca ccgaggaggt ctccgacaag atctcgtccg tgctcacctt catcgacgag
     1261 gttctcttcc acgtcagccg cggcgtccgc tgggatagcg accacgtggt caccctctcg
     1321 gatccggtgc gcgccgtcgc cgaggagtcc gtgcgcggca atgtcctcga ccgggtgcac
     1381 tttggcctgg actatttcga tgccagcatc aaccggatcg cggcatgggt catcggcgcg
     1441 cgctctctcc tgaaggcgct cctcatcgcc ctcgtcgagc ccacgcagct gctgaaggag
     1501 atggagctca atggcgaccg caccggccgc ctggcactga tggaagagct gaagatgttg
     1561 cccttcgcgg cggtctggaa ccgttactgc gagatgcagg gcgttcccgt cggggccgcc
     1621 tggctcaacg aggtccgcgc ctacgagcgc agcatcctct ccgagcgctg agatacgctt
     1681 ctctggcccc ccttccctgg aagcgggaaa gtttctccca ccccacgccc ccccagggaa
     1741 ggggggccgc gccctggcaa gtggccggcc gccgttccag gagcgttttc acccatcccc
     1801 gcccgaacgg gctctctggt cacgcgcctg cctgcgcgcc gcgatcagcg cgaggagcgt
     1861 caccgccgtc agtggaatga caaagccggc catcgcccgc gtgtagcctt ccagtgcctg
     1921 gctctccgtc cctcgcagat agaggtaagc gacgaaagtg acgtagtatc ccaggaagaa
     1981 cgcgccttcc caccgctcga gctgcgcgcc gatcacaaag accggcagga gggccacggc
     2041 cgccgtgatc atgatcggca tatccaggcg caccgcctcc agcggcaccg ccagtccgcc
     2101 gggagagagg agagcggcgg tgcccagcac ccccatgatg ttgaaaagac tgctgccgat
     2161 gacgttgccc acagtgatat caggatcgcc acgcctcgct gcaagggcgg atgtcgccaa
     2221 ctccgggagc gtcgtgccaa tggcgacgat tgtcaggctg atcaccagct cgctcacacc
     2281 gaaggcccgg gcgaaggaga cagcggcatt tacgaccagg gccgagcccg cagccaacag
     2341 gaggaggccc aatacgacga gggcgatatg ccggagccag ccgtcgtgcc cggcaacctg
     2401 cacttctgtc agggcggcgg gatacccggc ttcctccccg ctctcccgcc cccgccggat
     2461 cgcatgccgc gtgtaggcaa taaggcccaa gatcagaagc gccccctcat agcgctgcag
     2521 ccggccatcc accgagagga ggaagagcgc cagcgagacg accgccagca gcggcacatc
     2581 gcggcgcacc agctcctgcg agaggcagat cggcgccaaa agcgacgaga cgccgaggat
     2641 caggagaa
//
LOCUS       DAOSCV010000642         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25336_length_2634_cov_4.538969, whole genome shotgun sequence.
ACCESSION   DAOSCV010000642 DAOSCV010000000
VERSION     DAOSCV010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25336_length_2634_cov_4.538969"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /gene="grpE"
                     /locus_tag="PLY56_14400"
     CDS             <1..533
                     /gene="grpE"
                     /locus_tag="PLY56_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ22716.1"
                     /translation="EPESERRGDERIAELEARVAELEAALEQRTQEAEAREKEAKEFL
                     EGWQRERASFENFRKRMARELDEAGTVAKVDLIRAILPAVDNLERALDDESTSADVVR
                     QGVRLIHEQLLTALQKQGLKEVPTTGETFDPNVHEAIDAVENTEYAPDTVVEPLLKGY
                     TFGERLVRPARVRVAR"
     gene            812..1780
                     /locus_tag="PLY56_14405"
     CDS             812..1780
                     /locus_tag="PLY56_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ22717.1"
                     /translation="MSTFKDYYEVLGVKRDATEKEIKQAFRKLARKYHPDVNPGDPAA
                     AEKFKELSEAYEVLSDPEKRKAYDHFGAQWKQAQQAGARGFDFQNPYGGTVHFDLGGD
                     QDINEILRNIFGLGGGFGEGGAARGGRGGFGFGDLFGSAFRDRGGMGGAPVRGADVAA
                     EMEITLEEAYHGGAKQVQINGRRVEVKIPAGVDTGARLRLGGMGERSQHGGPPGDLYL
                     TVRVRPHPVFQRDGDNLRCDVQISFATAALGGEATVRTLRGGTVQMKIPAGTQGGQVF
                     RLGGLGMPKRGGSGYGDLLARVNIIVPRDLSDHERELVEELARQRG"
     gene            1786..2121
                     /locus_tag="PLY56_14410"
     CDS             1786..2121
                     /locus_tag="PLY56_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HOJ22718.1"
                     /translation="MGEDRRDEPLFLISVVARICDMHPQSIRLYERLGLIRPRYISKR
                     RFFSEADIERLRQIRRLTQEMGVNLAGVEIIFSLLDKIDQLQQELEDIKAEHRRATGA
                     FLLPASPDE"
     gene            complement(2240..>2634)
                     /locus_tag="PLY56_14415"
     CDS             complement(2240..>2634)
                     /locus_tag="PLY56_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22719.1"
                     /translation="EMPQQFGGLNNLNAGDRPARVEAVEAQGAVWLKREAKGWRLVPC
                     GNLGPWDHFPAEGLPEFHKDMRLKSIPADRGCGAIAVDTRALLSKAPAQVRIQARNEA
                     YQPATAKVQAGERLSFTPEAGVTDYLLE"
BASE COUNT          470 a          783 c          950 g          431 t
ORIGIN      
        1 cggagccgga gagtgagcgg cggggtgacg agcgtatcgc cgagctcgaa gcgcgcgtcg
       61 cggagctgga agcggccctg gagcagcgaa cacaggaagc cgaggcacgc gagaaggaag
      121 cgaaggagtt cctggaagga tggcagcgcg agcgcgcgag ctttgagaac ttccggaaac
      181 gcatggcacg cgaattggac gaggccggca ctgtggccaa ggtggacctg atccgggcga
      241 ttctcccggc ggtggataac ctggagcggg cgctggacga tgagagcacg agcgccgatg
      301 tggtgcgaca gggtgtccgc ttgatccatg agcagctcct cacggcactg cagaagcagg
      361 gattgaaaga ggtgccgacc accggcgaga cgtttgatcc aaacgtccac gaggcgattg
      421 acgcggtaga gaatacagag tatgcgccgg acaccgttgt ggagcctctc ttgaaggggt
      481 acaccttcgg tgaacggctg gtgaggccag ctcgcgtgcg agtggctcgc taagcggagg
      541 atgacgatgg gatgggcggt gagcggaacg cccaacggaa tgaatgcatg cctgcggcgt
      601 gctttccgcc ccgtcgcaga ttccgtgtag agtacccgca tcgagcgaaa ctccgttctg
      661 ccctggctcg gggccagtgt gagcctggcc gtatgcgcgg agcgcgggca ctgagtgggt
      721 tgggatggag acgaggacgc cgtgagggat gaggagggcg aggcgaacgc cggttgcccg
      781 ccccctctcc cttgtcagat ccggggagaa gatgtcgaca ttcaaggact actacgaggt
      841 gttgggcgtg aagcgggacg cgacggagaa agagatcaag caggcgttcc gcaagctcgc
      901 gcgcaaatac cacccggacg tgaaccccgg cgaccccgct gctgccgaga agttcaagga
      961 gctgagcgag gcctacgagg tgctgagcga cccggagaaa cggaaagcct atgaccactt
     1021 cggggcgcag tggaagcagg cccagcaggc gggtgcccgc ggcttcgact tccagaaccc
     1081 ctatgggggc acggttcact tcgatctggg aggcgaccag gatatcaacg agatcctgcg
     1141 caatatcttc ggcctgggcg gcggctttgg cgagggtgga gcagcgcgcg gcggtcgcgg
     1201 cggcttcggc ttcggtgatc tcttcgggag tgcgtttcgc gatcgggggg gcatgggagg
     1261 agctcctgtc cgcggtgccg atgtcgccgc cgagatggag atcactctcg aagaggcgta
     1321 tcatgggggc gcgaagcagg tccagatcaa cggccggcgc gtggaggtca agatccccgc
     1381 tggcgtggat actggagccc ggcttcgcct gggtggcatg ggcgagcgca gccagcacgg
     1441 cggccccccc ggcgacctct atctgacggt gcgcgtccgc ccgcacccgg ttttccaacg
     1501 cgacggcgac aatctgcgct gcgacgtaca gatcagtttc gcgacggctg ccctgggggg
     1561 cgaggcaacg gtgcgcaccc tgcgcggtgg caccgtccag atgaagattc cggctggcac
     1621 ccagggggga caggtgtttc gcctgggcgg tttgggaatg cccaagcgag gtggttcggg
     1681 ctatggcgac ctgctggcac gggtcaacat tatcgtgccc cgtgacctga gtgaccatga
     1741 gcgcgagctc gttgaggagc tcgcccggca gagagggtga aaaggatggg ggaggaccgc
     1801 cgggacgagc cgttattcct gatcagcgta gtcgcacgga tctgcgatat gcatccgcag
     1861 agcatccggc tctacgaacg attggggctc attcgtccac gctacatcag caagcgccgc
     1921 ttcttctccg aggcggatat cgagcgcctt cgccagatcc ggcgcctgac ccaggagatg
     1981 ggcgtgaacc tggcgggggt ggagatcatc ttcagcttgt tggacaagat cgaccagctc
     2041 cagcaagagc tcgaggatat caaggcggag caccgtcgcg ccacgggcgc tttcctgctt
     2101 cccgccagtc cggatgagtg attccttcgc ttcgcgcgaa cgctttgggg catggggcgg
     2161 cgcgggcgcg gagccgccgg gaaggccgct cgatggcaga ggccctgcca ccgggcggcc
     2221 tttttccgtg gtatcgcgct tactccagca ggtaatccgt cacccctgcc tcgggcgtga
     2281 agctcagcct ctcgcccgcc tgaaccttgg cggtggcagg ctgatacgct tcgttgcgcg
     2341 cctggatgcg cacctgcgcg ggagccttgc taaggagcgc acgcgtgtcg accgcgatgg
     2401 cgccgcaccc gcggtccgcc gggatgctct tcaggcgcat gtccttgtgg aactccggca
     2461 ggccctcggc cgggaagtgg tcccagggcc ccaggttgcc gcacggaacc aggcgccacc
     2521 ccttcgcttc gcgcttcagc cacaccgcgc cctgcgcctc gactgcctcg acgcgcgccg
     2581 ggcggtcgcc ggcgttgaga ttgttgaggc ccccgaactg ctgcggcatt tcac
//
LOCUS       DAOSCV010000643         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25339_length_2634_cov_3.160527, whole genome shotgun sequence.
ACCESSION   DAOSCV010000643 DAOSCV010000000
VERSION     DAOSCV010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25339_length_2634_cov_3.160527"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..718)
                     /locus_tag="PLY56_14420"
     CDS             complement(86..718)
                     /locus_tag="PLY56_14420"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOJ22720.1"
                     /translation="MSDGTREGTEGTRRRGHRVWRILGALGLAAFLGVAIPAHFIDRY
                     GQGDRARPANAIVVLGARVDERGEPGDSLRARTLHAVSLYKRGLAPKILCTGGIGDYP
                     PSEAAAAAALARAHGVPAADLFLEEQSTSTRENALYAAKICREQGWGEVIVVSDPYHL
                     WRARREFTRAGLIVHVSPARECARNQVLHRRFYWAAREVLMLARDVLRGM"
     gene            complement(868..1242)
                     /locus_tag="PLY56_14425"
     CDS             complement(868..1242)
                     /locus_tag="PLY56_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOJ22721.1"
                     /translation="MRARTSNRGSRFGRMMDEMAAILWLVALSLLILSGARAATSVLK
                     HQRDQRYPQSVVIEVKRGDCLWSIAKRFSRPGEDPRRMVHILSVANRISTGQVLYPGD
                     RLVVPYVETRAPSDTGAVLARR"
     gene            1594..>2634
                     /locus_tag="PLY56_14430"
     CDS             1594..>2634
                     /locus_tag="PLY56_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22722.1"
                     /translation="MGCLLALLGVGAAMGAAEAGPAATGAGSFQTLAAITLDEAPWSA
                     VLVPEEGPPRDGAPTQALRWPVAEQPGPDSPVLLTDWSGVDAVRFWLHLDKPCPFRLN
                     VLLTGEMNGYFMTSIPLDFTGWRQFTLPIESFGKVREADLAAARRLSFRAQGYGQPEI
                     EKTTVWWVDQVEARPKPGVKLPIAPIGPAATRAAWEALAREGNPYLILNARRYQAELP
                     PFRPPAEIRSAWQYRGVAEQLVPLAYAVADPASPHRGREDLLEHALGLVDWLTAQCHD
                     EGWWWRQGPLPGDPNVNRFTLSPLLDGIRWLRLLPEGEAAWPRWRDTLAKAIDLQRRA
                     YRHEEAWDWAGLA"
BASE COUNT          452 a          885 c          858 g          439 t
ORIGIN      
        1 caccatccca cggctgaaga aaacgaacgg tagatggctg tgaatagcga tcgctcatga
       61 gatctcctac tccgcctgcc cggcgctaca tccctcgaag aacgtctcgg gcgagcatta
      121 acacctctcg cgcggcccag tagaaacgcc ggtgcagcac ctggttgcgc gcgcactcgc
      181 gagccgggct gacgtgaacg atgaggccag ctcgggtgaa ctcccgcctc gcccgccaca
      241 ggtgataggg gtcgctgacc acgatgacct ccccccatcc ctgctcacgg cagatcttcg
      301 ccgcgtagag agcgttctcc cgcgtgctcg tagactgctc ctcgaggaac agatctgccg
      361 ccggcacgcc gtgcgcgcgg gccagcgccg cggcagcggc ggcttcgctc ggcggataat
      421 cgccgatgcc acccgtgcaa aggatcttcg gcgccagccc acgcttgtag agggagacgg
      481 cgtgcagcgt gcgcgcgcgc aggctgtcgc ccggctctcc tcgttcatca acgcgcgcgc
      541 ccaatactac gatggcgttc gccggccgcg cccgatctcc ctgtccatag cggtcaatga
      601 agtgcgcggg gatggccacc cccaggaatg cggccagtcc gagcgcgccc aaaatgcgcc
      661 aaaccctgtg cccacggcgc cgcgttcctt ccgtgccctc tcgggtgccg tcactcattt
      721 tcgcatacct tcccgtttcc atcgaggcat gagggttgcc atgacggcac tccgcaagaa
      781 gccggcgcct tcacgcgccc gcgagaccag ggtgcgttgc cgatagaccc acaggagggc
      841 cgtggaatac ctgtcgaggg acggggatca gcgccgggcg agaaccgccc cggtatcaga
      901 gggagcgcgc gtctcgacat acggaacaac gaggcggtcg ccagggtaga ggacctggcc
      961 agtagagatc cggttggcaa ccgagaggat atgcaccatg cgccggggat cctcgccagg
     1021 acggctgaag cgcttggcaa tactccacag gcaatcgccc cgcttcacct caatcacgac
     1081 gctctgcgga tagcgctggt cgcgctggtg cttcaacacg ctggtagcag cgcgcgcccc
     1141 ggagagaatg aggaggctca aagcgaccaa ccagaggatc gccgccatct cgtccatcat
     1201 tcggccaaac cgggagcccc tattgctcgt gcgtgctctc atagtcacca cccggctgcc
     1261 tactcggctg ccaccggtat cataccagat gttgggtatt gcgtcaaggc cgtcggctag
     1321 atatagtggc gatgcctctt gcgccccgcg gtggtcgccc aggtggtaca cctcggatcg
     1381 ctaattgcgg cggagagaac gagacgagaa ggcgcatcct gttctctatg ctcccgcccc
     1441 gccccgtccc gccgcactgg cagggcactc cgcgcgccgt ggcgaatagc tccgcaatcg
     1501 cgggggattc ccctcgccct gtgtgccata tcgacctggt gcgccgcagt gagcaacgca
     1561 tcggggtgaa agggaatcgg tatggaggct ctgatgggat gcctattggc tcttctgggc
     1621 gtcggggcag cgatgggtgc ggcggaggcg ggtccggcag ccactggcgc cggctcattc
     1681 caaacactcg cagccatcac attggacgag gccccctggt cggcggtgct cgtgccggag
     1741 gagggacctc cgcgcgacgg tgccccgacg caggcactgc gctggcccgt ggctgagcaa
     1801 ccggggccgg actcgcccgt gctcctcacc gactggtctg gggtggatgc ggtgcgtttc
     1861 tggctgcatc tggataaacc gtgtcccttc aggctgaacg tgctcctcac cggtgagatg
     1921 aacggctact tcatgacgag catcccgctc gatttcaccg ggtggcggca gttcacgctt
     1981 cccatcgagt cgtttggcaa ggtgcgtgag gccgatctgg cggcagcacg gcgtctctcc
     2041 ttccgggcgc agggctacgg gcagccagaa atcgagaaga caacggtgtg gtgggtggat
     2101 caggttgagg cgcggcccaa gccgggggtc aagctcccca tcgccccgat tggaccggcg
     2161 gctactcgcg ccgcctggga ggcgctagcc cgggagggca atccctatct gatcctgaac
     2221 gcccggcgct atcaagcaga gcttccgccc ttccggccgc ctgcggagat ccgcagcgcc
     2281 tggcaatacc gcggcgtcgc cgaacagctg gtgcctctcg cctacgccgt cgccgatccg
     2341 gcatcgcccc accgggggag ggaggacctg ctggagcatg ctctcggact cgtcgattgg
     2401 ctcaccgcgc agtgccacga tgagggctgg tggtggcgcc aagggcctct cccaggtgat
     2461 cccaacgtga accgcttcac gctgagcccg ctgttggatg gtatccgctg gctgcgcctg
     2521 cttcccgagg gagaagccgc atggccacgc tggcgcgaca cgctggcaaa ggcaattgac
     2581 ctgcaacgcc gggcctaccg gcacgaagag gcgtgggatt gggccggact ggcc
//
LOCUS       DAOSCV010000644         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25399_length_2630_cov_3.332816, whole genome shotgun sequence.
ACCESSION   DAOSCV010000644 DAOSCV010000000
VERSION     DAOSCV010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25399_length_2630_cov_3.332816"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1113
                     /gene="hemB"
                     /locus_tag="PLY56_14435"
     CDS             121..1113
                     /gene="hemB"
                     /locus_tag="PLY56_14435"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOJ22723.1"
                     /translation="MSEFPRVRMRRLRVTEAHRRLVRETHLSTADLIYPLFVVEGTGI
                     QREIAAMPGNYHWSVDRLPYLMDEIASLGIPGVLLFGVPDAKDEVGSSAYAEDGIVQR
                     AIRAIKRSHPDLLVITDVCLCEYTSHGHCGVVGSDGQVQNDATLALLQRTAVSHAQAG
                     AEIIAPSDMMDGRIGAIRAALDEHGFEQVAILAYAAKYASGFYGPFREAAGSAPQFGD
                     RRAYQMDPANRREALREIALDVAEGADMVMVKPALAYLDIIRETRDRFDLPLAAYNVS
                     GEYSMVKAAAANGWIDGDRIAMEILLGIKRAGADLILTYHALEAARRLKEGWGL"
     gene            complement(1110..>2630)
                     /locus_tag="PLY56_14440"
     CDS             complement(1110..>2630)
                     /locus_tag="PLY56_14440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ22724.1"
                     /translation="EYAVLACQQEPYDPIDRALVSFANEQLARTEHLHADWTLVREYP
                     LSPELLALSHVWLSPEGEEYIIAAKGAPEAVADLCHLAPGELREMEAVVSDMAEDGLR
                     VISVAAASFRRGELPGEQHDFPFEFLGLIGLVDPIRPDAPKALDECRQAGIRVVMITG
                     DYPGTAIHVARQIGLTPADEVLTGPEMAEMDDAQLRERARTTSIFARVVPEQKLRLVN
                     ALKANGEVVAMTGDGVNDAPALKAAHIGIAMGGRGTDVARESAALVLLDDDFSSIVHA
                     VRLGRRIFDNLRKAMAYVLSIHVPIAGMSLVPVLAQLPLVLLPAHIAFLELIIDPASS
                     TVFEAEAAEEGIMRRPPRSRAEALFNRRMVLLSVLQGIGVFLVVLAVFAVARYRGLTE
                     SEARALTFTTLIFSNLGLIYTNRSWSRTIPALLRTRNTALWAVTGVTLLALALVLYVP
                     LLRDLFDFAPLGGWEVVLALVAGVLSILWFEVLKVMVRTPIQAIRERPRRQELRAR"
BASE COUNT          448 a          830 c          913 g          439 t
ORIGIN      
        1 cctggcgcgc gatagattag tgtggggctg gcaggcgatg cctgccagcc ccttttcaag
       61 tacacgccgt cccgtggttg cgtggggcgg caagcggttg caaccgcggg aggaagtcac
      121 atgtccgagt ttccacgagt gaggatgcgc cggctgcgcg tgaccgaggc gcaccgccgc
      181 ctggtgcgcg agacgcacct ttccaccgct gaccttatct accccttgtt cgtcgttgag
      241 ggcactggca tccagcgtga gattgccgcc atgcccggca actaccactg gtcggtcgac
      301 cggcttccct acctgatgga tgagatcgcc tcgctcggca ttccgggagt gcttctcttc
      361 ggcgtgccag atgcaaagga cgaggtgggc tcttcggcct atgcggaaga cgggattgtc
      421 cagcgcgcca tccgcgctat caaacgctcg cacccggatc tgctggtgat taccgacgtc
      481 tgcctctgtg agtacacctc gcacggccac tgcggcgtgg tgggctctga tggtcaggtc
      541 cagaatgatg ccacgctcgc cttgctgcag cgcaccgcgg tttcgcacgc gcaggccggc
      601 gcggagatca tcgctccatc agacatgatg gatggtcgca tcggggcgat ccgggcggcg
      661 ctcgacgagc acggcttcga gcaggtcgcg attctggcct acgcggcaaa gtacgcgtcg
      721 gggttctatg gcccgtttcg ggaagccgcg ggctcggcgc cgcagtttgg cgaccggcgc
      781 gcctatcaga tggacccggc gaatcggcgc gaggcgttgc gcgagatcgc cctcgatgtg
      841 gcggagggcg ccgacatggt gatggtcaag ccggcactcg cctacctcga catcatccga
      901 gagacgcgcg accggtttga tctccccctg gccgcctaca acgtctccgg cgagtactcg
      961 atggtgaagg cggctgcggc gaacgggtgg atcgatggcg accggatcgc catggagatc
     1021 ctgctcggga ttaagcgggc cggcgcggat ctgatcctca cctaccatgc gctcgaggcg
     1081 gcgcgccggc tgaaagaagg ctggggcctc tagcgagccc gaagctcctg cctccgaggg
     1141 cgctcgcgaa ttgcctgaat cggcgtgcgt accatcactt tgaggacttc gaaccacagg
     1201 atgctgagca cgcccgccac gagggccagc accacttccc agccacccag aggcgcgaag
     1261 tcgaacaggt cgcgcaagag gggcacatag aggaccagcg cgagggcgag gagagtcacc
     1321 ccggtcactg cccagagcgc cgtattgcgc gtgcgcagga gcgcggggat ggtgcggctc
     1381 caagagcggt tggtgtagat gaggcccagg ttcgagaaga tcagcgtggt gaaggtgagc
     1441 gcgcgcgcct cggactcggt gagcccgcgg taacgggcga ccgcgaagac cgccagcacg
     1501 accaggaaga cgcctatacc ttgcaggacg ctgagaagca ccatccggcg gttgaaaagt
     1561 gcctctgccc gggagcgcgg gggccggcgc atgattccct cctcagctgc ctccgcttca
     1621 aagacggtgg aggatgccgg gtcgatgatt agctccagaa aggcaatgtg cgccgggaga
     1681 aggacgagag gcagctgggc caggacggga acgagcgaca ttccggcgat tggcacgtgg
     1741 atggagagga cgtaggccat cgccttgcgc aggttatcga agatgcgcct ccccagccgg
     1801 accgcgtgga cgatggacga gaagtcatcg tcgagcagga cgagggcggc ggactctcgg
     1861 gcgacatccg tgccccggcc ccccatggcg atcccgatgt gagccgcctt cagcgccggg
     1921 gcgtcgttca cgccgtcacc cgtcatcgcc acgacttcgc cgttggcctt gagcgcgttc
     1981 accaggcgca gcttctgttc cggaacgacg cgcgcgaaga tgctggtggt gcgtgctctc
     2041 tctcgtagtt gtgcgtcgtc catctccgcc atctccggcc cggtgaggac ctcgtcggcg
     2101 ggagtgagcc cgatctggcg ggcgacgtgt atcgcggtgc ctggatagtc gccggtgatc
     2161 atcaccacgc ggatcccggc ctggcggcat tcgtccagcg ccttgggggc gtccggccga
     2221 atcggatcga cgaggccgat caacccgagg aactcaaacg ggaagtcgtg ctgctcgccg
     2281 gggagctcgc cacgccggaa gctggctgcc gccacgctga tgacgcgaag gccatcctcc
     2341 gccatgtcgc tgaccacggc ttccatctcg cgaagctcgc cgggagcaag gtggcagaga
     2401 tcggccacgg cttccggggc tcccttggcg gcgatgatat actcctcgcc ctccggggag
     2461 agccagacgt gcgatagcgc caggagttcc ggcgagaggg ggtactctcg gaccagcgtc
     2521 cagtcggcat ggaggtgctc ggtgcgcgcc agctgctcgt tggcgaagct caccagcgcc
     2581 cggtcgatcg ggtcgtaggg ctcttgctgg caggccagga cagcgtactc
//
LOCUS       DAOSCV010000645         2630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25403_length_2630_cov_2.886990, whole genome shotgun sequence.
ACCESSION   DAOSCV010000645 DAOSCV010000000
VERSION     DAOSCV010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25403_length_2630_cov_2.886990"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="PLY56_14445"
     CDS             <1..1378
                     /locus_tag="PLY56_14445"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ22725.1"
                     /translation="VAGIYLDQIAAARPRICFDPSHPHAPGSGEAWIEQGYWPMLEVI
                     RRDLRQKYPNVIFNSEDAAEPYMHVLDGYLPWRFLDIGHVPAYQAIYAGRIQMTSRSY
                     SDTAYDALFPKAAEQMLYGEQIWWFPVSLVNSNPAFKLFVKKLAHTRRTFLPFFNEGD
                     MLKPPPFASEMPTITADWGFYGPRIITTPAVLHSAWRLGDSVAVIFANTSCDPITAQV
                     APDAAAWGLGAKPVRVTQFTEGRDPEVAEWRTGQPLRLTVPGYSMAAWLFAAGNANDA
                     RYQAQANAARELFRALKRFEDEAGLTPEQKRALHAAQNPWIAPDVPARSATEWIPATE
                     APKLIGARKAPDGSFVGWITASSAICFGPIDFGADTGGKPVIECELAVPDNVTNARIR
                     FMEAVEGRGVPIAQARLQKTGGYDQYATVRLPLVPGVAGRKNIVIQFEGRGGGLCNIR
                     RWRLVREP"
     gene            1451..1975
                     /locus_tag="PLY56_14450"
     CDS             1451..1975
                     /locus_tag="PLY56_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001885245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOJ22726.1"
                     /translation="MMRGLTRALLATREENKATLPLRIPLAMIFVAHGAQKLFGWFGG
                     HGLDATIEGFESLGIPPGLTKLAATTEFLGGLGVLTGFLTRPAALGIATVMAVATAKV
                     HWQHGFFVNWGNQPGRGHGIEATMALAGMALSLVISGGGALSVDAALTHAKPEEKEES
                     GESTAKRGQETLCA"
     gene            complement(2098..>2630)
                     /locus_tag="PLY56_14455"
     CDS             complement(2098..>2630)
                     /locus_tag="PLY56_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22727.1"
                     /translation="DTQNARLRETFGQGGFEVVIGGPHTSYFKEPDKEGIIDLQHQLM
                     LQVLAHPLVDVLVHPWWFSASEFRPGGPMEWLTDMTQIPDWHTRELAETALKYGKAIE
                     ANYSAFYTEKQYGEAFRETYTHYLAEIARRGVKLSIATDSHDINRLDGIHKMMEILES
                     VGITREQLWTPEMKAA"
BASE COUNT          442 a          836 c          908 g          444 t
ORIGIN      
        1 cgtagccggg atctacctgg atcagatcgc cgcagcccgg ccgcggatct gcttcgatcc
       61 gtcgcatccg cacgcgcccg gatccggcga ggcgtggatc gagcagggct actggccgat
      121 gctcgaggtg atccggcgcg atctgaggca gaagtatccc aacgtgatct tcaacagcga
      181 ggatgccgcc gagccctaca tgcacgtgct cgatggctac ctgccgtggc gcttcctcga
      241 tatcggccat gtgccggcct accaggcgat ctacgccggg cgcatccaga tgaccagccg
      301 gagctacagc gacacggcgt acgacgcgct cttcccgaag gctgccgagc aaatgctgta
      361 tggcgagcag atctggtggt tcccggtgag cctggtgaac tccaacccgg cgttcaagct
      421 gttcgtgaag aagctggcgc atacgcggcg caccttcctg ccgttcttta acgagggcga
      481 catgctgaag ccgccgccgt ttgcgtccga aatgcccacg attaccgcgg attgggggtt
      541 ctacggcccg cggatcatca cgacgccggc ggtgctccac agcgcctggc ggcttggcga
      601 cagcgtcgcg gtgatcttcg ccaatacgtc gtgcgatccg atcacggccc aggtggcgcc
      661 ggacgccgcg gcctgggggc tgggggcgaa gccggtgcgc gtgacgcagt tcaccgaggg
      721 aagagatccc gaagtagcgg agtggcgcac gggccagccg ttgcgcctga cggtgccggg
      781 ctacagcatg gcggcgtggc tcttcgccgc cggcaacgcg aatgatgctc gctaccaggc
      841 gcaggcgaat gccgcgaggg aactgttccg cgcgctgaag cggttcgagg acgaggcggg
      901 gctgacgccc gagcagaagc gcgccctgca cgctgcccag aacccctgga tcgcaccgga
      961 cgtgccggcg cgttctgcca cagaatggat ccccgcgacc gaggcaccca agctgatcgg
     1021 ggcgagaaag gcgcccgatg ggagcttcgt gggctggatc accgcgtcga gcgcgatctg
     1081 ctttggcccg attgacttcg gcgccgatac cggtggcaag ccggtgatcg aatgcgagct
     1141 ggcggtgccg gacaacgtca ccaacgcccg aatccgcttc atggaagcgg tggagggaag
     1201 gggtgtgccc atcgcccagg cgcggctgca gaagaccggt ggctacgacc agtatgccac
     1261 ggtacggctc ccccttgtcc cgggcgtggc cggacgcaaa aacatcgtca tccagttcga
     1321 ggggcggggc ggcggcctct gcaacatccg ccggtggcgc ctagtgcgcg agccgtagcc
     1381 gagaccgagt ttcggctagg cgccttgggg ggaagaagcc cgacaagcgg atcgcttcta
     1441 ggaggggacg atgatgcgcg gtttgacccg cgccctgctg gcgacacggg aggagaacaa
     1501 ggcaaccctg ccgctgcgca tcccgctcgc gatgatcttc gtcgcccatg gcgcgcagaa
     1561 gctcttcggg tggtttggcg gtcacggcct tgacgccacg atagagggct tcgagtcgct
     1621 gggcatcccc ccggggctga ccaagctcgc cgcgacgacc gagttcctgg gggggctggg
     1681 ggtgctcact ggcttcctga cacgcccggc ggcgctggga atcgccacgg tgatggcggt
     1741 cgccacggcg aaggtccatt ggcaacacgg gttcttcgtg aactggggca accagccggg
     1801 ccggggacat ggaatcgagg ccacgatggc cctcgcgggg atggcgctat cgctggtgat
     1861 ctccggcggt ggcgccctct cggtggacgc ggcgctgacc cacgccaaac ccgaggagaa
     1921 ggaggagagc ggcgagagca ccgccaagcg cggtcaggag acgctctgtg cctgagtgga
     1981 atacacgact gaacgggcgt gaggatgcgg cgtgtgctcc ttgcacgccc gttcagtcag
     2041 gcgttcttca ggatccagca ggcgccatcg gatgaggaag cgcctgctgg cccgcctcta
     2101 cgcggccttc atctccggcg tccaaagctg ctcgcgggtg atcccgacgc tctccaggat
     2161 ctccatcatc ttgtggatgc cgtcgagccg gttgatatcg tgtgagtcgg tggcgatgct
     2221 gagcttcacg ccccgacgcg caatctcggc gaggtagtgg gtgtaggtct cgcggaacgc
     2281 ttccccatac tgcttttcgg tgtagaaggc gctgtagttc gcctcaatcg ccttgccgta
     2341 tttcagcgcc gtctcggcca gctcgcgcgt gtgccagtcg gggatttgcg tcatgtcggt
     2401 gagccactcc atcggcccgc cgggccggaa ctcggacgcg ctgaaccacc acgggtgcac
     2461 cagcacatcc accagcgggt gggcgagaac ctggagcatc agctggtgct gtagatcgat
     2521 gatcccttct ttatccggct ccttgaagta ggacgtgtgc ggcccgccaa tcacgacctc
     2581 gaagccgccc tggccgaacg tttcccgcag gcgcgcattc tgcgtgtcga
//
LOCUS       DAOSCV010000646         2627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25440_length_2627_cov_4.026827, whole genome shotgun sequence.
ACCESSION   DAOSCV010000646 DAOSCV010000000
VERSION     DAOSCV010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25440_length_2627_cov_4.026827"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLY56_14460"
     CDS             complement(<1..1092)
                     /locus_tag="PLY56_14460"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ22728.1"
                     /translation="MRASARRDHGLIKTDASPHCKLRSVNLRSVRWAKGFWSNRFRQC
                     REVTLPHLWALLSDRKPGDALANRHLAAGLKEGDFTGSPWQDGWIYKWIEAASSFYAA
                     TGDRRLDAQMDEVIEEIARAQPPDGYLALESSVRDEPRSYDTHHHALHVIGHLITAAC
                     IHHRVTGKQSLLNIARRAADHVWATFRECGPKLARLLFHPTIIMAGVELYRTTGVSRY
                     LEMANHFIDSRGALPGGTDQAQDFLPLRREREVVGHMVLATHLYAGAADAYMETGDPS
                     LLEALERLWQDLTEHKLYVNGGCCAYHRALSVCWPGDGGSPTSAYEVSGAAGPAYDLP
                     NATAHNEACAQIGSVMWNWRMLAITGEARY"
     gene            1998..>2627
                     /locus_tag="PLY56_14465"
     CDS             1998..>2627
                     /locus_tag="PLY56_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22729.1"
                     /translation="MCRFITLACLLVAMAGVAGAEEIFRDGFDAPSDAAPLKQTWGDA
                     PAHVEVNAVVPGAGVGGGPGARLRLAYPEELKHRLSYFTYTLKEPMPVIPELKEISFR
                     VKANVPVHLKVPIGPYGFIYHAPGAGPSQEWQRVALARAYDELKAWCDRGGRSVEGAF
                     ITGIIVAVVPTKGGVAEVSIDEITMAGSEGARAAAREERIRRRTRKVRVS"
BASE COUNT          431 a          845 c          880 g          471 t
ORIGIN      
        1 gtaacgcgct tcgcccgtga tggcgagcat gcgccagttc cacatcacgc tcccgatctg
       61 cgcgcacgcc tcgttgtggg cggttgcgtt cggaaggtcg taggcggggc cagcagcgcc
      121 actcacctca tatgcgctcg ttggagaccc gccatcgcca ggccagcaca ctgagagcgc
      181 gcggtggtag gcacagcacc cgccattcac gtatagcttg tgctcggtca agtcctgcca
      241 cagccgctcg agcgcctcca agagcgaggg atcgcccgtc tccatgtagg catccgccgc
      301 gcccgcgtag agatgggtgg caaggaccat gtgccccacc acctctctct ccctgcgcaa
      361 ggggaggaag tcctgggcct gatctgtgcc gccgggcagg gcgccccggg aatcgatgaa
      421 gtggttggcc atctccaggt agcgcgacac tcccgtcgtc cggtagagct cgacgcccgc
      481 catgatgatc gtggggtgga agaggagccg tgccagcttt ggcccgcatt cacgaaaggt
      541 ggcccagacg tgatcggcgg cccttcgggc gatgttgagg agactttgct tgcccgtcac
      601 gcggtgatgg atgcacgccg cggtgatcag atggccgatc acgtgcagcg catggtggtg
      661 ggtatcatag gagcggggct cgtcgcgcac gctcgattcc aacgccaggt aaccgtccgg
      721 tggttgcgcg cgcgcgatct cctcgatcac ctcatccatc tgggcatcca gcctccggtc
      781 gcccgtagcc gcatagaagc tagacgccgc ctcgatccac ttgtagatcc atccatcctg
      841 ccaggggctg ccggtaaagt caccctcctt caggccggcg gcgaggtgcc ggttcgcgag
      901 cgcgtcgccc ggcttccggt cgctcaacag ggcccacaga tggggaagag tcacctcgcg
      961 gcactggcga aagcggttgg accaaaagcc ctttgcccac cgcaccgagc gcagattcac
     1021 actgcgcagc ttgcagtgcg ggctggcgtc tgtcttaatc agcccgtggt ctcgcctggc
     1081 ggaagcgcgc atatacggtc acctctcctg cttcactcga acagggcgcg gccgctgctc
     1141 cactcctctc gggtgcttgt gttagagacg agtaacaccc ggcagcggtt cgcgcgcagg
     1201 gaatgaacgg cggtgttgcg tgccatggct gtgggtgacc cggcgtctgg agggtgttcg
     1261 cgaacgatgg cgagcgaacg agggggctgc ccgggcgcgt tccgatgtgg aactctcccg
     1321 gctgcatcgc ggccgagtgg ccagccaggc atggagagag gaagtgccgt gacgcgtgcc
     1381 tttagtctcc cagcttccgc gtccaggctc aatgatgaga tcaccctggt tctcctccag
     1441 aatcgtgtcg cggacgtggg accccgggca ggtgtaggcg cccgaggcgg tcagcatcga
     1501 tcgggaacag ccttcagttc gctaccgagc gagcatctcc tccagttctt atacagatat
     1561 atacaaatat atgcgccccg gctctcagcc ggtgttcgtt atcctggcat cacagaccat
     1621 ccgccacggc cgcgcaccgg tcctctcccg gcccggaagg ggccacacag cacagcccag
     1681 ggtgtctgca ccctgggtaa cgctcccctt cactggggcc ggccagatag cgcggcggta
     1741 aatcaccgcg ctgcttgtga acgaggccct acgggctggt tcgcggagcc ccgaaggggc
     1801 ttcgccggtc ggcagcgcga agcttcagat ccgcgcctgt agattccagg cgctcgcacc
     1861 ctccccatcc gtgtacgtct tcttcatccg tggtatcgat ggcatcgccg acgggggccc
     1921 ggaaggtccg gtctctctcc tcgacgaatc ctcctctcca cggaagagag cttttctgct
     1981 ccggaggggt cacggccatg tgtcgcttca tcacgctcgc gtgtctgctc gttgccatgg
     2041 ccggcgtggc cggcgccgag gagatcttcc gggatggctt cgatgccccg tcagacgcgg
     2101 cgcctctcaa gcagacctgg ggcgatgcgc cggcgcacgt agaggtgaac gctgtcgtac
     2161 cgggggcggg cgttgggggc ggtccgggcg cgcgcctgcg cctggcttac ccggaggagc
     2221 taaagcaccg gctgagctac ttcacgtaca cgctgaagga gccgatgccg gtcatcccgg
     2281 aactgaagga gatctccttc cgggtgaagg cgaacgtgcc ggttcatctg aaggtgccca
     2341 tcggccccta cgggttcatc tatcacgcgc cgggcgccgg ccccagccag gagtggcagc
     2401 gcgtggcgct cgcccgggcg tatgacgaac tgaaggcgtg gtgcgaccgg ggagggcgtt
     2461 cggtggaggg ggccttcatc acgggtatca tcgtggccgt cgttccaacg aaaggcgggg
     2521 tggcagaagt gtcgatcgat gagatcacaa tggcggggtc ggaaggggcg cgtgctgcag
     2581 cgcgcgagga gcgtattcgc cggcgcacgc gcaaggtgcg cgtcagc
//
LOCUS       DAOSCV010000647         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25476_length_2625_cov_3.439689, whole genome shotgun sequence.
ACCESSION   DAOSCV010000647 DAOSCV010000000
VERSION     DAOSCV010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25476_length_2625_cov_3.439689"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(783..2045)
                     /locus_tag="PLY56_14470"
     CDS             complement(783..2045)
                     /locus_tag="PLY56_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ22730.1"
                     /translation="MDWVWAAGMVGATGSEGGHDVTGLLLTVLVIFVAAKIGAEIFER
                     LKQPAVVGEILAGVIVGPQVLHWVEPSEVTFALSELGVIFLLFMVGLETKPSDLLAVG
                     RQALLVAVGGVVLPMVLGYFFALHYGTTQVTALFIGAALVATSVGITARVLARMGYLH
                     TRTARIILGAAVIDDILGLLVLAVVSGFARTGAADIRQIVLTGFYAVSFVLFMLFFGC
                     RVMARALPAIDRLRIGQSVYLGAIGLCLTLSVVAGYLGVAAIVGAFLAGMALSEASEE
                     IGLHRHFDNLSEFFIPFFLVGIGMQLNLSSLANPSVLAMCLILTLLAILAKVIGCSLP
                     VLSMGGLRAMQVGVGMVPRGEVGIIVAQLGLGLGALNDDLFAVVLFVAVTTTMVAPPL
                     LAPLYKSEPRVLHVDEDATTAEVGVDIS"
     gene            2246..>2625
                     /locus_tag="PLY56_14475"
     CDS             2246..>2625
                     /locus_tag="PLY56_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAA hydrolase family protein"
                     /protein_id="HOJ22731.1"
                     /translation="MKLATFERNGTRRVGAVLGEDRIVDLAAAAAGSAAEKILDAGML
                     ALLDAGEPGLAAAREAAEKAPASAILPLADVRLCAPILYPRKLFCLAGNYAEHIQEGG
                     RNVEEKDKVTPRVFMKPPTTTVVGP"
BASE COUNT          516 a          901 c          804 g          404 t
ORIGIN      
        1 aagatcaggg agctcattca ccatctgcgt ctccctttgc gcgcctctct tccactcgcg
       61 cgaggaactc gttcccaaag ctgtctctcg gtttctggat ggagccaggc gttcctgcca
      121 gacggtttcc cggggccctg gacgggcagt caaggggctc gaaacgggcg ccggcgaagc
      181 gatcctgtgg ttccacacca acccatcgta agaatctcac gggcgccggc aaagcctgcg
      241 gtcgcttcgc cggcgcccgc gcagcgccag tcatttgccg ccgtgccgtg gagagagagg
      301 ccacaccgcg gcgtcactcc tcttccggca actcggcggg ctgtccatcc cggaccacgg
      361 agcgatagca gcgttcgcac agctcctcat ggtccccacc atgcgctccc cgccggtatc
      421 gccggtggcg gctgggggta ccgcagcgcg cgcacctccc gagggagcta ccggtcaaca
      481 tgcgagtcag gcctgccatg tcttcgtcca tagctacctc ccgatgatgg tgccctgatc
      541 gcacctctcg cagacgggtg ggtcgtcctc ctatgtagcc agcggggctg aaaggcgcgt
      601 ctcccctctc cccccgggaa agcccgaccc gacccttcct tgctgagaac tgacgcctga
      661 gtatactacc catctgcggg gcaggtaagc ccaaactctg ggccggaatg cgcgatatcg
      721 aaccggcacc cttgcatccg ttcactcgcg cactccacag accactccat cgacctaccc
      781 gttcaggaga tatcgacccc cacctcggcc gtggtcgcat cctcatccac atgcaacaca
      841 cggggttcgg atttatagag cggggcgagt agcggcgggg ccaccatggt ggtcgtcaca
      901 gcgacgaaga gcacaaccgc gaagagatca tcgttcagcg cgcccaaacc caacccgagc
      961 tgggcgacga tgatgcccac ctcgccgcgc ggcaccatgc cgactcccac ctgcatggcg
     1021 cgaagaccgc ccatggacag cacgggcagc gagcagccaa tgaccttcgc gaggatggcc
     1081 agaagcgtga ggatcaggca catcgccagc acactggggt tggccaggct gctaaggttg
     1141 agctgcatcc caatgccgac cagaaagaag gggatgaaaa actccgagag gttgtcgaaa
     1201 tggcggtgga gaccgatctc ctcgctcgcc tccgagagcg ccattcccgc aaggaacgcc
     1261 ccaacgatgg ccgccacgcc caggtaaccg gcgacaaccg aaagcgtcag gcagaggccg
     1321 atagcaccca ggtagaccga ctggccgatg cgcaggcggt caatggcggg caacgcgcgg
     1381 gccatcacgc ggcaaccgaa gaagagcatg aagagaacaa agctaaccgc gtagaagcct
     1441 gtcagtacaa tctgccggat atcggcggcg ccggtgcgcg cgaagccgct gacaaccgcg
     1501 agcaccagga ggccaaggat gtcgtcaatg accgcggctc cgaggatgat gcgcgccgtg
     1561 cgcgtgtgca ggtagcccat gcgcgccagc acgcgcgccg taatgcccac gctcgtcgcc
     1621 accagggccg cgccgatgaa tagcgccgtc acctgcgtgg tgccgtagtg caacgcgaag
     1681 aagtagccaa gcaccatcgg cagcaccacc ccaccgacgg caaccaacaa cgcctgccgg
     1741 cccacggcaa ggagatctga gggcttcgtc tcgagcccga ccataaacag aaggaagatg
     1801 acccccaact ccgaaagcgc gaaggtgact tccgagggtt ccacccaatg caacacctgc
     1861 gggccgacaa tgacgccagc caggatctct cccacgacgg cgggctgctt gagccgctcg
     1921 aagatctccg cgccgatctt cgcggcaacg aagattacca gcaccgtgag cagcagaccc
     1981 gtcacatcat gcccgccctc gctcccggtt gcgcccacca tgccagcagc ccacacccag
     2041 tccattcgct ctcccttcac acgcgctgtg ccggcaccgt gcgacgccgg cctagggact
     2101 tgttcgcctg ttggtcaggc gcgtccttct gggcacggcg cctgtcctgg tcacgccttg
     2161 gcatgggctt gcctcgggtg cccctcatgt gctatgcttg gagggaagat ttggattggc
     2221 accgttctga tgaaggggta ctgctgtgaa gctggcgaca tttgagcgca acggaactag
     2281 gcgcgtgggc gccgttctgg gtgaggaccg cattgttgac ctggcagcgg ccgctgcagg
     2341 cagcgccgcc gagaagatac tggacgcggg gatgctcgcc ctgctggacg ccggtgaacc
     2401 ggggctcgcg gccgctcgcg aggccgccga gaaggcgccg gccagcgcca ttctcccatt
     2461 ggctgatgtg cggctttgcg cgccgatcct ctacccgcgc aagctgttct gcctggccgg
     2521 caactacgcc gagcatatcc aggagggcgg gcggaacgtc gaggagaagg acaaggtcac
     2581 gccgcgcgtc ttcatgaagc cccccaccac cacggtggtc ggacc
//
LOCUS       DAOSCV010000648         2623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25506_length_2623_cov_3.228972, whole genome shotgun sequence.
ACCESSION   DAOSCV010000648 DAOSCV010000000
VERSION     DAOSCV010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25506_length_2623_cov_3.228972"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLY56_14480"
     CDS             <1..886
                     /locus_tag="PLY56_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOJ22732.1"
                     /translation="ARCWELPVVANRRFSGRYRELVLDAPELAASVRAGQFVSILCST
                     VDDPLLRRPFSVYRVDPGAGTIGILYEVLGRGTSLIARWERGDIANVLGPLGNYFTVP
                     KGEGPLALVGGGVGVAPLLALAHEVAPSGRPIHAILGARTASMLLGVDEFEALGVRVS
                     ISTDDGSQGYHGFVSGLLASLLQGGAGREPLRPDAIFTCGPRPMMAAIAEIARAHGIP
                     CSVSMEVSMACGFGVCMGCAWPVRSGSDSDAGPTYRLVCTDGPVFNADEIIWEPRQAR
                     CACAGGKAEGTGEEHDHA"
     gene            879..1838
                     /locus_tag="PLY56_14485"
     CDS             879..1838
                     /locus_tag="PLY56_14485"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOJ22733.1"
                     /translation="MREPAKPTHPPAMPAVDLSVEIGTLRLRNPVTTASGTFGYGREM
                     ADLVDLSRLGAITVKTLQLHPRPGNPPPRICETPAGMINSIGLPGSGIEHFLKEDLPF
                     LRGYGTPVILSVAGSRVEEYVALVERVHGEEGIAAIELNISCPNVKQGGLAFSVDPHA
                     TAQVVSACRAATARPLIAKLTPNVTHIATIARAAADAGADAIAAINTIQAMAIDVRSR
                     RPRIATNIGGLSGPAIRPIAVRMVWEIAQAVSIPIIGMGGITSAADALEFILAGATAV
                     AVGTATFRSPGAVIEVIDGLGPLLAELGVARIRDLVGAVQLWE"
     gene            1829..>2623
                     /locus_tag="PLY56_14490"
     CDS             1829..>2623
                     /locus_tag="PLY56_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HOJ22734.1"
                     /translation="MGMIYRLRDVRLEPEGRVQDLCLRDGHLEECADGLPGVEIEGNG
                     GLLLPGMIDPHVHLRTPGMEAAENWDTGTRAAARGGVTTVLDMPNTRPPTTTRQRLAE
                     KRALAAAARVNWGLHFGATATNLPEIAAVENIASVKAYQGSSTGDLLLDEDEALARLF
                     KAAATADMPVTLHAEDEGIIRERAASLPEDADVHLHGWARPPEAAASAVKRALALAQE
                     TGATLYFCHVSTAAELELIRPAKAAGMPIYVEATPHHLFLDERDLAT"
BASE COUNT          399 a          921 c          875 g          428 t
ORIGIN      
        1 cgcccgctgc tgggaactcc cggtggtggc aaaccggcgc ttcagcggcc gctatcgaga
       61 gttggtgctc gatgcgcccg agctcgccgc ttccgtgcgg gcgggccagt tcgtcagcat
      121 cctctgctcg acggtagacg atccgctgct caggcgaccc ttcagcgtct atcgcgtgga
      181 tcccggcgcg ggcaccattg ggatcctcta tgaagtgctc gggcgcggca cctcgctgat
      241 cgcgcgctgg gagcgcggcg atattgccaa cgtgcttggc cctctcggca actacttcac
      301 agttcccaag ggcgagggcc ccctcgccct cgtcggaggt ggcgtcggcg tcgcgcctct
      361 cctcgccctg gcccatgagg tggcgccctc cggaaggccg atccatgcca tcctcggcgc
      421 gcgaacggcg tccatgctcc tcggcgtgga tgaattcgaa gcgctcggcg tgcgggtgag
      481 tatctccacc gatgacggat cccagggcta ccacggcttc gtcagcgggc tgctcgcctc
      541 cctgctccag ggaggggcgg gccgcgagcc gcttcgcccc gacgcgatct tcacctgcgg
      601 gccccgcccg atgatggccg caattgcgga gattgctcgg gcccacggaa tcccctgctc
      661 cgtctcgatg gaggtgagca tggcgtgtgg atttggcgtc tgcatgggct gcgcctggcc
      721 ggtccggtcg ggctccgact cagatgcggg ccccacctac cggctggtgt gcaccgacgg
      781 gcccgtcttc aacgcggatg agatcatctg ggagccccgg caggcccggt gcgcatgcgc
      841 cggaggcaag gccgaaggca caggagagga gcacgaccat gcgtgagccg gcgaagccaa
      901 cgcatccccc cgctatgccc gcggtggatc tttccgtcga gatcggcacg ctgcgcctgc
      961 gcaacccggt caccacggca tcgggcacgt ttggctacgg acgcgagatg gcggatctcg
     1021 tggatctcag ccgcctcggg gcgatcacag tgaagaccct ccagcttcac cctcgacctg
     1081 gcaacccgcc accgcggatc tgcgagaccc ctgccgggat gatcaactcg attggattgc
     1141 ccggctccgg catcgagcac ttcctcaagg aggacctgcc cttcctgcgc ggctatggca
     1201 ccccggtgat cctcagcgtg gccgggtcgc gcgtggaaga gtatgtcgcc ctcgtcgagc
     1261 gcgtccatgg cgaagaggga atcgccgcga tagagctcaa catctcctgc cccaacgtga
     1321 agcagggtgg gctcgccttc tccgtggacc cgcacgccac cgcgcaggtc gtttccgcct
     1381 gccgggccgc gacggcgcgc cccttgatcg ccaagctcac tcccaacgtc acccacatcg
     1441 cgaccatcgc gcgagccgcc gccgatgcgg gcgcggacgc gatcgccgcg atcaacacca
     1501 tccaggcgat ggcgattgat gtacgctcgc gccggccacg catcgccacg aatatcgggg
     1561 gtctctcggg gccggccatt cggccgattg ctgtccgcat ggtctgggag atcgcccagg
     1621 cggtctccat cccgatcatc ggcatgggtg gaatcaccag cgccgccgac gccctggagt
     1681 tcatccttgc cggcgcaact gcggtggccg tcggcacagc caccttccgc agcccaggag
     1741 ccgtcatcga agtgatcgac ggccttggcc ccctcctggc cgaattgggc gtcgcgcgta
     1801 tccgcgatct ggtgggagcg gtgcagctat gggaatgatc taccgcttgc gggatgtccg
     1861 tctggagcca gagggtcgtg tgcaggacct ctgcctgcgc gatggccatc tggaggagtg
     1921 cgcggatggg ctgcccggcg tcgagatcga ggggaatggc gggctcctct tgccggggat
     1981 gatcgacccc cacgtccatc tccgcacgcc ggggatggaa gccgcggaga attgggacac
     2041 gggtacccgc gcggcggcac gtggcggggt caccaccgtg ctcgatatgc cgaacacgcg
     2101 cccgcctacc accacgcggc aacgcctggc cgagaagcgc gcgttggcgg ccgctgcccg
     2161 ggtgaactgg gggctccatt tcggcgcgac ggccaccaac ctgccggaga tcgccgcggt
     2221 cgagaacatc gcctccgtga aggcgtacca gggctcctcc accggcgatc tcctcctcga
     2281 cgaggatgag gcgctggcgc gtcttttcaa ggcagcagcg acagccgaca tgcccgtaac
     2341 cctgcacgcg gaagatgagg ggatcattcg cgagcgtgcc gcatccctcc ccgaggatgc
     2401 cgatgtccac ctgcatgggt gggcgcgtcc ccccgaggcc gcggcaagcg ccgttaagcg
     2461 ggcgctcgcg ctggcgcagg agacgggggc aacactctat ttctgtcacg tctccaccgc
     2521 cgcggagctc gagctgatcc ggcccgcgaa ggccgccggg atgcccatct acgtcgaggc
     2581 gacgccccac catctcttcc tcgacgagcg ggatctggcg acg
//
LOCUS       DAOSCV010000649         2622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25516_length_2622_cov_3.548890, whole genome shotgun sequence.
ACCESSION   DAOSCV010000649 DAOSCV010000000
VERSION     DAOSCV010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25516_length_2622_cov_3.548890"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLY56_14495"
     CDS             <1..881
                     /locus_tag="PLY56_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HOJ22735.1"
                     /translation="SSGQTLCVVNMKRHALALVDLLRERGVERLRHLSTSMCPAHRAA
                     VLSEIKGWLDPAHPEPCCLVATQCIEAGVDVDFATVYRALAPLDAIAQAAGRCNRHGR
                     RPVGDVVVFRPDDEERLYPTDAYQQAALVTDSLLRRRGPEGMDLYDPELFATYYRELY
                     DLARPQEQREALRDAIRAQNFPEVARLYRLIEKDAVNLLVRYPEAESEVEALAAEVRR
                     EGLTASWVRRARPYSVAIYRPDRAMLQSSFLEPVPLRGKGRAGEAQDWFLCLDPECYD
                     PDLGLNLPAGSRSLVF"
     gene            994..1764
                     /gene="cas5c"
                     /locus_tag="PLY56_14500"
     CDS             994..1764
                     /gene="cas5c"
                     /locus_tag="PLY56_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOJ22736.1"
                     /translation="MTPNRQLLEVWGDLACFTRPELKTERVSYPLITPSAARAIFDAI
                     YHKPKFIWQVRRIEVLPPRHGSAAWDASGAPRFIALRRNEMKDHSWSTDEIIQWALGK
                     AEPKPLFADADKEVLGTDERGRTQRQTMALRDVRYRLHAEIRPWPEFQSELPAMEAQF
                     RRRAAAGQCIYQPYLGCREFPAYFRLCEDKPAEPAAPINLDVGWMLYDVFDLSRPGSS
                     TDAACISFFRAVVRSGVLDVPEYASDEVRKPYGRGNHD"
     gene            1757..>2622
                     /locus_tag="PLY56_14505"
     CDS             1757..>2622
                     /locus_tag="PLY56_14505"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOJ22737.1"
                     /translation="MIDRLVSYARSEGLIAEPGFTPKEVKWAVDISDGRYAGLLSLGD
                     PELKRHSGRRFPCCPDLSQPELTVFKGVGTHFLVAACDQVALYPEPDPRKREGCKTLQ
                     KHAFFIRLLRDASEDVPQLAAAASVLEDPSVLEKLRADLAAHKAKATDRITVRIDGDF
                     PVESDAWHPWWRRFRMSIAERKAETRKRPSQGRPMVCLATGQLVEPEPTHPKISGLVD
                     VGGQSAGSVLIGYDKAAFRSYGLSQSENGAMSAETATAYREALNHLLSHHSRKLVGAK
                     VVHWYQGRAKVE"
BASE COUNT          494 a          820 c          826 g          482 t
ORIGIN      
        1 agagctctgg acagacgctc tgtgtcgtca atatgaagcg acatgccctc gctctcgtgg
       61 atctgctacg cgagcgcgga gtcgagcgtc tgcggcatct atcgacgagc atgtgccctg
      121 cgcatcgcgc tgcagttctc agcgagatca agggatggct ggatcccgcc cacccggagc
      181 cctgttgtct tgtggcaact cagtgtatcg aggcgggagt cgatgttgat tttgccaccg
      241 tctatcgtgc gctcgcgcca ctagatgcta ttgcccaagc cgctggccgg tgcaatcgtc
      301 acggacggcg gccagtgggc gatgtggtcg tcttccgacc agacgacgag gagcgtcttt
      361 accccactga tgcctaccaa caggcggcgc tcgtgaccga ctcgctgctg cgccggcgcg
      421 gaccagaggg aatggatctt tacgaccccg agctgttcgc cacctactat cgcgagctgt
      481 atgatctggc ccgcccacag gagcagaggg aagccctacg ggatgcgatc cgcgcccaga
      541 acttccccga ggtggcacgc ctctaccgcc tgatcgagaa ggatgccgtc aatctgctgg
      601 tacgctaccc ggaggccgaa tccgaggtcg aggctctcgc cgcggaagtg cgccgcgagg
      661 gcctgactgc cagctgggtg cgccgggctc ggccctattc ggtcgccatc taccgcccag
      721 atcgcgccat gctccagagc agctttctgg agccagtgcc gctgcgtggc aagggccggg
      781 ccggagaggc tcaggactgg ttcctttgct tagatcccga atgctacgat cccgatctcg
      841 gcctgaatct tccggccggc tcgcggagtt tggtcttcta ggcatgtttg ccgtatggag
      901 tggccgttta tggaacgtat ctaagaagaa tctgctcgtt gcccggcggc tccgccgggc
      961 aacgaatcgc cgcatgcgaa aggaggaacg gatatgaccc ccaaccgcca gctcctggaa
     1021 gtctgggggg acctcgcgtg cttcactcga cctgaactga agacggagcg ggtgtcttac
     1081 cccttgatca ccccctcggc ggcgcgcgcc atcttcgatg ccatctacca caagccgaag
     1141 ttcatctggc aggtgcgccg catcgaggtg ctgccaccac ggcatggcag cgctgcttgg
     1201 gacgcctctg gagcaccgcg cttcatcgcc cttcgacgca atgagatgaa ggatcacagc
     1261 tggagcaccg acgagatcat ccagtgggcg cttggaaaag cggagcccaa acccctcttt
     1321 gcggacgctg acaaagaggt gctgggcacg gatgaacgcg ggcgaacgca gcgccagacg
     1381 atggcgctga gagacgtgcg ctatcgcctc catgccgaga tacgcccctg gccagagttc
     1441 cagagcgagc ttccagcgat ggaagctcag ttccgacgcc gggcggctgc cgggcagtgc
     1501 atctatcagc cctacctcgg ctgccgcgag ttccccgcct acttccggct gtgcgaagat
     1561 aagcccgcag agcccgccgc gcccatcaac ctcgacgtag gctggatgct ctacgacgtc
     1621 ttcgatctgt cacgccctgg cagcagtacc gatgccgcat gcatcagctt cttccgagcg
     1681 gtggtccgtt ctggggtgct ggatgtaccc gaatacgcca gtgacgaggt gcgcaagcca
     1741 tacggaaggg ggaaccatga ttgaccgcct ggtgagctat gcgcgctccg agggcctgat
     1801 cgcagagcct ggcttcacgc ccaaagaggt gaagtgggcc gtggatatct cggatgggcg
     1861 ctacgcgggt cttctatcgc tgggagatcc cgagctgaag cgccattcgg gccgccgatt
     1921 tccctgctgc ccggatctct cacaacccga gttgacggtg ttcaaaggcg tgggaaccca
     1981 tttcctggta gccgcctgcg accaggttgc cctctaccca gagcctgatc cgcgtaagcg
     2041 ggaaggttgt aagacgctac agaagcacgc cttcttcatc agactgctcc gcgacgcatc
     2101 cgaggatgtt ccacagctag cggccgcagc gagcgttctg gaagatcctt cagtcctgga
     2161 gaagttgcgc gccgatctgg cagcgcataa ggcgaaggca accgacagga tcaccgttcg
     2221 gatcgatgga gacttcccgg tcgaaagcga tgcctggcat ccctggtggc ggcgattccg
     2281 catgagtatc gcagagagaa aggccgagac caggaagagg ccgagccagg gccggcctat
     2341 ggtttgcctt gccacgggcc agctggtgga gcccgagccg acacacccga agatcagcgg
     2401 tctcgtcgat gtgggcggcc aatcggcagg cagcgtgctc attggctacg ataaggccgc
     2461 cttccgctcc tacggtctca gccaatccga gaacggcgct atgtcggccg agaccgcgac
     2521 tgcctatcga gaggcgctga accaccttct cagtcaccac agccgcaagc tggttggtgc
     2581 gaaggtggtc cattggtatc aggggcgcgc caaggtggag aa
//
LOCUS       DAOSCV010000650         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25643_length_2613_cov_2.754887, whole genome shotgun sequence.
ACCESSION   DAOSCV010000650 DAOSCV010000000
VERSION     DAOSCV010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25643_length_2613_cov_2.754887"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PLY56_14510"
     CDS             complement(<1..459)
                     /locus_tag="PLY56_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOJ22738.1"
                     /translation="MNLPTLTDVVASLLAWVGGALGLGTLSGLVPLVVYVLDALIIVM
                     LVIFPVVTVVIWLLRKVMGFIQRRLGPMRTGPVGVFQTIADAIKLFHKEDIIPASADR
                     AVFLIAPFLVFVPAVMLYVVIPFGKGGFITKDLNVGLVYLTAISSITAVGL"
     gene            complement(569..1777)
                     /locus_tag="PLY56_14515"
     CDS             complement(569..1777)
                     /locus_tag="PLY56_14515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOJ22739.1"
                     /translation="MSKTDRDERGELQDALPRVGGSAVPLDPSGELHEIETDHIELYM
                     GPQHPSTHGVLQLKVRLDGEWVVDVQPHIGFLHRGVEKIAENRTYVQVLPLTDRLDYV
                     AAIACNTAYCVAVEKLLGITIPPRAELCRVIVQELERIASHLISVGTFGLDLGATTPF
                     IYAFRDREYVLDLLEMICGARLTYSYVRVGGVAQDLPEGEWRERFRWFLDYFPPMVDE
                     QDTLLSENEIFLLRTRGIGVLPPDVAINWGSSGPMARGSGVAYDLRRSKPYGLYPQLD
                     FNVVTRETGDALARYQVRIDEMRESIKILRQCDRLLDEIPPGEIMAKGARAVRPPAGE
                     AYAAVESPRGEMGAYIISDGSNTAYRMKWRSPSFANLSVLPVISRGWKIADLVMILGS
                     IDPIFGDVDR"
     gene            complement(1943..2464)
                     /locus_tag="PLY56_14520"
     CDS             complement(1943..2464)
                     /locus_tag="PLY56_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOJ22740.1"
                     /translation="MADEKKIARPASDEAADPFSEVRRGLQQRFGEDVAVTLAPGGDL
                     VVTVGRERLLAVCRYLKEEQGFDYPILVTGVDRGDGLESVIHLGQMTSPRVIVLKTPV
                     SYDDPQVPSVTSIWAGADWHERESYDLMGIRYEGHPDLRRILLPEEWDEYPHPLRKEF
                     KLDSPGGKFSITE"
     gene            complement(2485..>2613)
                     /locus_tag="PLY56_14525"
     CDS             complement(2485..>2613)
                     /locus_tag="PLY56_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOJ22741.1"
                     /translation="DQIVPVDMYVPGCPPRPEALLDGVIKLHEKIRREKYRATWKA"
BASE COUNT          484 a          872 c          835 g          422 t
ORIGIN      
        1 aagcccgacc gcggtgatcg acgagatcgc cgtcagatag accaggccaa cgttcaggtc
       61 cttggtaatg aacccgccct tgccgaaggg gatgaccacg tagagcatca ccgcgggcac
      121 gaacaccagg aaaggcgcga tcaggaagac cgcccggtcg gcgctcgcgg ggatgatgtc
      181 ctccttgtgg aacagcttga tggcgtcggc gatcgtctgg aaaacgccca caggtcctgt
      241 gcgcatcggg cccaaccggc gctggatgaa acccatcact ttgcgcaaga gccagatgac
      301 caccgtcacg accgggaaga tcaccaacat gacgatgatc agggcgtcga gcacgtagac
      361 gacgagcggc accaggcccg agagcgtgcc caggcccaga gcgcctccta cccaggcgag
      421 gagggaggcg acaacgtcgg ttaaagtcgg caggttcacc agctacctcc ccggcgtcgg
      481 gcgcgccacc cagggcgtga ccagggggcc ggccgatact caagcctcgc ctcgcgctcc
      541 gaaatcccca attcgcgctc cgcgtgtctc accggtctac gtctccaaag atcgggtcga
      601 tgctgccgag gatcatcacc aggtcggcga tcttccagcc gcgagagatg actggcaaca
      661 ccgagaggtt ggcaaaggag ggagaacgcc acttcatccg gtaggcggtg ttgctcccat
      721 cactgatgat gtaggcgccc atctcacccc ggggcgactc cacggcggca taggcctcgc
      781 cggcaggcgg acgcacggcg cgcgcgccct tggccatgat ctcgcccggc gggatctcat
      841 cgagcagccg gtcgcactgg cgcaagatct tgatgctctc gcgcatctcg tcgatgcgca
      901 cctggtaacg cgccagcgcg tcgcccgtct cgcgcgtgac cacgttgaaa tcgagttgcg
      961 ggtaaagtcc gtacggcttg ctgcggcgca ggtcataggc cacgccggat ccgcgcgcca
     1021 tggggccgct ggacccccaa ttgatggcaa catcgggcgg gagcacgccg atgccgcggg
     1081 tgcgcagcag gaagatctcg ttctcgctga ggagcgtgtc ctgctcgtcc accatgggcg
     1141 ggaagtagtc caggaaccag cggaaccgct cgcgccactc accctcgggc agatcctggg
     1201 cgacgcctcc cacgcgcacg taagagtagg tgaggcgcgc cccgcagatc atctccagca
     1261 ggtcgagcac gtactcgcgg tcgcgaaacg cgtagatgaa gggggtggtg gctcccaggt
     1321 cgaggccaaa ggtgcccacc gagatgaggt ggcttgcgat ccgctccaac tcctggacga
     1381 tgacccggca aagctccgcg cgcggcggaa tggtgatccc cagcagcttc tcgacggcga
     1441 cgcagtaggc cgtgttgcag gcgattgccg ccacgtaatc gagacggtcg gtcaggggaa
     1501 ggacctggac gtaggtgcgg ttctcggcga tcttctcgac gccccggtga aggaagccga
     1561 tgtgcggctg aacatccaca acccactcgc catccaggcg caccttgagc tggagcacgc
     1621 cgtgcgtgct ggggtgctgc gggcccatat agagctcaat gtggtccgtc tcgatctcgt
     1681 ggagctcgcc actgggatcg agcggcacgg cagagccacc cacgcgcggg agggcgtctt
     1741 gcagctcccc tctctcgtct cggtcagttt tgctcatctg tattccagga gtcgtgaaac
     1801 ggcactcgaa aagcgcccgc caacgcccgg cggcaaccat ccctcgcata gcgcggcatg
     1861 cgaaccgcgc ccggcgttca gcgggcccac ggggtcactg ttgggagcgc gccttcccgg
     1921 gcagggggcc gtgccccact tctcactcgg tgatactgaa cttcccgccc gggctatcca
     1981 gcttgaactc tttgcgtagc ggatggggat actcgtccca ctcttcagga agcaggatgc
     2041 ggcgcaggtc gggatgcccc tcgtagcgga ttcccatcaa atcgtatgac tcgcgctcgt
     2101 gccagtcggc tcccgcccag atcgaggtaa cgctcggcac ctgggggtcg tcatacgaaa
     2161 ccggcgtctt caggacgatc acccgcggcg aggtcatctg gcccaggtga atcaccgact
     2221 ccaggccatc gccacggtcc acgccggtga ccagaatcgg gtagtcgaac ccctgctcct
     2281 ccttcaggta gcggcagacc gcaagcaggc gctcgcggcc cacggtcacg acgagatcac
     2341 cgccgggcgc gagggtcacc gcgacatcct cgccaaagcg ttgctgaagc ccgcggcgga
     2401 cctcggaaaa gggatccgcg gcctcgtcgg acgccgggcg ggctatcttc ttctcatctg
     2461 ccatgagctg cggccttgct cccctcaggc tttccaggta gcgcggtact tctcgcggcg
     2521 gatcttctcg tgcagcttga tcacgccatc caacagcgcc tctggccggg gcggacaccc
     2581 gggaacgtac atatcgaccg gcacgatctg gtc
//
LOCUS       DAOSCV010000651         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25662_length_2611_cov_3.403365, whole genome shotgun sequence.
ACCESSION   DAOSCV010000651 DAOSCV010000000
VERSION     DAOSCV010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25662_length_2611_cov_3.403365"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PLY56_14530"
     CDS             <1..1514
                     /locus_tag="PLY56_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22742.1"
                     /translation="ECLRSGESIPWGAWVVPLAAWSILVVAIFFGFLCLAALLRRQWA
                     DNEKLAFPLAQLPLEMARSGDAFLRNPLTWIGFAIPAMIFTLNGIHQLFPTVPGVNLN
                     LSLNRYFPNPPLSAIYPTPLHFSMAAIGFFYFLPTDLLFSLWFFFVLTRVQDVVAATF
                     NAPIENMPLYPTRIYVGYQVLGAYVVLAGYLLYVARPHLRQVWDAVFGRAKVDDSQEL
                     IPYRVAFWGLTGSVLVSATWFMLAGLSWWVAALEILAYFFVIALVMARSVAECGMLMT
                     ETSFRPIDMHSLFAARHTLGASNLTALAFLDAAFLRDQRGLILTGFLDGLRVADGVNI
                     RRRAFLPVFGLAIGLAILVAGFLHLWFPYQKGGIQLYSYVYAGNPLLAFRNHAPAVEN
                     PVNFDWRAPTFAAIGMAVTLFLVIMRMTFYWWPLHPMAYALSASWSLIVFWFPCFVAW
                     VIKSLVQRYGGMKYYLFFRPFFLGMILGEFSMAVVWTVLAAAFDVPAPSVPWP"
     gene            1571..>2611
                     /gene="tgt"
                     /locus_tag="PLY56_14535"
     CDS             1571..>2611
                     /gene="tgt"
                     /locus_tag="PLY56_14535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ22743.1"
                     /translation="MFELLATDTKSAARRGRMDLGRVVVETPVFMPVGTQATVKAMTP
                     DEVWEIGYRLILANTYHLYLRPGADLIERAGGIHRFMAWKGGVLTDSGGYQVFSLRDL
                     RRIGDDGVCFRSHLDGTEHTFTPESVMRIEEQIGADVIMAFDECPPYPTTREYARESM
                     IRTHAWAKRCREAHQTDSLLFGIVQGSVYPELRRESAEYIASLDFPGNAIGGTSVGEP
                     REKMLEAVEICAPLLPANKPRYLMGVGMPLDILDAVARGMDMFDCVLPTRMGRNGTAF
                     TTYGRANIKAARYREEFGPLDPECPCPVCQRYSAAYLRHLYKAGEILSARLLTYHNLA
                     FYYRLMAGIREAL"
BASE COUNT          397 a          869 c          784 g          561 t
ORIGIN      
        1 acgagtgcct gcgctccggc gagtcgatcc cctggggcgc atgggtggtg ccgctcgccg
       61 cctggagcat cctcgtcgtc gcgatcttct tcggcttcct ctgcctggcg gcactcctgc
      121 gccggcaatg ggcagataac gagaaactcg ccttcccact ggcgcagctc cccctggaga
      181 tggcgcgcag cggggatgct ttcctgcgca acccgctcac ctggatcggc ttcgccattc
      241 cggccatgat cttcaccctg aatggaatcc accagctctt cccgacggtt cccggcgtga
      301 acctcaactt gagcctcaac cgctacttcc ccaatccgcc cttgtccgcg atctacccca
      361 cgccgctcca cttctcgatg gccgcgatcg gcttcttcta cttcttgccc acggacctgc
      421 tcttctcgct gtggttcttc tttgtgctca cccgcgtgca agacgtggtg gcggcaacct
      481 tcaacgcgcc tatcgagaat atgccgctct atcccacgcg gatctacgtt ggataccagg
      541 tcctcggcgc ttatgtcgtc ctcgcggggt atctcctcta tgtggcgcgc ccgcacctgc
      601 gccaggtgtg ggatgccgtg ttcgggcggg caaaggtaga tgactcccag gaactcattc
      661 cctaccgcgt cgccttctgg ggactcaccg gaagcgtcct cgtctccgcg acgtggttca
      721 tgctcgccgg cctctcatgg tgggtcgccg cgttggagat cctcgcctat ttctttgtga
      781 tcgccctggt gatggcacgc agcgtggccg agtgcggcat gctgatgacg gagacttcgt
      841 tccggcccat cgatatgcac tcgcttttcg ccgcgcgaca caccctggga gccagcaacc
      901 taactgcgct tgccttcctt gacgcggcct tcctgcgcga ccagcgcggc ctgatcctga
      961 ccggtttcct ggacggcctg cgcgtggccg atggagtgaa tatccgccgg cgagcgtttc
     1021 tccccgtctt tgggctcgcg atcgggttgg cgatcctcgt cgccggcttt ctccatctct
     1081 ggtttcccta tcagaagggc ggaatccagc tctattcgta cgtctatgcc ggcaaccccc
     1141 tcctggcgtt ccgaaatcat gccccggccg tcgaaaaccc ggtgaacttc gactggcgcg
     1201 cgcccacctt cgcggcaatc ggcatggccg tcacgctctt tctggtcatc atgcggatga
     1261 ccttctactg gtggccgctc caccccatgg cctacgccct cagcgcctcg tggtcgttga
     1321 tcgtcttttg gttcccctgt ttcgtcgcgt gggtgatcaa gtcgctcgta cagcgctatg
     1381 gcggcatgaa atactacctc ttcttccggc cgtttttcct ggggatgatc ctgggtgagt
     1441 tcagcatggc cgtggtctgg acggttctcg cggcggcgtt cgatgtgccg gcacccagcg
     1501 tcccctggcc ctgatcgcca cgcacaggtt gccggaagag gagcgacatt ggtataatgg
     1561 caccatagcg atgtttgaac tacttgcgac tgatacgaag agtgccgcgc gacgcggccg
     1621 catggatttg ggccgggtgg tcgtcgagac gcctgtcttc atgccggtag gcacgcaggc
     1681 aacggtgaag gcgatgacgc cggatgaagt gtgggagatc ggctaccggc tcatccttgc
     1741 caacacatac cacctctacc tgcgcccggg cgcggatctc attgagcgcg ccgggggcat
     1801 tcaccgcttc atggcctgga aggggggcgt gctcaccgat tcggggggct accaggtctt
     1861 ctcgttgcgg gatctccgcc gcatcgggga tgacggggtt tgcttccgat cgcacctgga
     1921 tggcacggag cacaccttca ctcccgagag cgtcatgcgc atcgaggagc agattggcgc
     1981 cgatgtgatc atggcgttcg acgagtgccc gccctacccc actacccggg agtacgcgcg
     2041 cgagtcgatg atccgcacgc atgcctgggc gaagcgctgc cgcgaggccc accagaccga
     2101 ttcgctcctc ttcgggatcg tgcaggggag cgtttacccc gagctgcgcc gcgagagtgc
     2161 ggagtatatc gccagcctcg atttccctgg gaacgccatc gggggcacga gcgtaggcga
     2221 gccgcgcgaa aagatgctcg aagcggtcga gatttgcgcg cctctcctgc ccgccaacaa
     2281 gccgcgctat ctgatgggcg tcggcatgcc gctggatatc ctggatgccg tggcgcgggg
     2341 catggatatg tttgattgcg tgctcccgac gcgcatgggg cgcaacggca cggcgtttac
     2401 cacctacggg cgcgcgaata tcaaggccgc tcgctatcgc gaggagtttg gcccgctcga
     2461 tccggagtgc ccctgtcccg tctgccagcg ctactcggca gcctacctcc ggcatctcta
     2521 taaagccgga gaaatcctgt cggcgcgcct gctcacctac cacaacctcg cattctacta
     2581 ccggttgatg gccgggatcc gggaagcgtt g
//
LOCUS       DAOSCV010000652         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25677_length_2610_cov_4.444618, whole genome shotgun sequence.
ACCESSION   DAOSCV010000652 DAOSCV010000000
VERSION     DAOSCV010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25677_length_2610_cov_4.444618"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLY56_14540"
     CDS             <1..303
                     /locus_tag="PLY56_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22744.1"
                     /translation="PGTHFAMGATARLSNRRTGERMVEDEVRYLGQKARELLDAYERE
                     KPAHTLRTFAQVETLWENVVRPHLKEFMTLQDAWNNQEPPSESSRWRENWKVPEVY"
     gene            complement(297..1121)
                     /locus_tag="PLY56_14545"
     CDS             complement(297..1121)
                     /locus_tag="PLY56_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ22745.1"
                     /translation="MAMEEIDGIKHPAYKHALSLTRPAERMQSGLYLVETANFVRQAL
                     ASPAEVLSVFATRDEASSFSEECAAQDIPLYIASAGLIQKLVGTSYEVAVTAICVVRQ
                     RRVALESLGGKQTVILAGEEIQDPRNVGVLVRTAEAVGCAALLLSANSAEAYSRPAVR
                     SSTGSILRLPVCLVPDLPTALRSLRQRGFHVVASSARAPRTVYDGALSRRPLVVVVGN
                     ETNGMTEAARAEASEFVSLPMASGGPSSLNVTVAAGVLLYEALRQARAVREPGETQ"
     gene            1395..1470
                     /locus_tag="PLY56_14550"
     tRNA            1395..1470
                     /locus_tag="PLY56_14550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1428..1430,aa:Arg,seq:cct)
     gene            1880..2035
                     /locus_tag="PLY56_14555"
     CDS             1880..2035
                     /locus_tag="PLY56_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22746.1"
                     /translation="MAKGDEGKKSKNSRIGDAKANICRECGKPSKPVLMLDGGKKRLV
                     LTCHEKV"
     gene            complement(2135..2338)
                     /locus_tag="PLY56_14560"
     CDS             complement(2135..2338)
                     /locus_tag="PLY56_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22747.1"
                     /translation="MPRYRAQVPYTDDLGRERWEYVEVTASMHDPDELARAAKEEWER
                     RHPDRVGLLPEDLEWEEMGEDDW"
BASE COUNT          483 a          804 c          877 g          446 t
ORIGIN      
        1 ccaggaacgc actttgctat gggagcgacc gcgcgccttt ccaaccgccg gacgggcgag
       61 cgcatggtcg aggatgaggt gcgctacctt gggcaaaagg cgcgcgagct gctggatgcc
      121 tatgagcgcg agaagccggc gcacacgctg cgcacctttg cccaggtgga gacgctgtgg
      181 gagaacgtgg tgcgccccca tctgaaggag tttatgactc tccaggacgc gtggaacaac
      241 caggagccgc cgtccgagag cagccgctgg cgcgagaact ggaaggtgcc ggaagtctac
      301 tgagtctcgc cgggctcgcg caccgcgcgc gcctgccgga gagcctcgta gaggagcacg
      361 ccggcggcga cagtgacgtt gagcgatgac gggccgcctg aagccatcgg cagcgagaca
      421 aactcgctcg cttcggcacg ggcggcttct gtcatgccgt ttgtctcgtt gccgacaacc
      481 acgaccagcg gccggcgcga gagcgcgccg tcatagaccg tccgtggtgc ccgcgccgag
      541 ctggcgacca catggaaccc gcgctggcgc agcgaccgga gggctgtcgg gagatcggga
      601 accaagcaca ccggcagccg gaggatcgat ccggtggagg atcgcaccgc cgggcgagag
      661 tacgcctcgg cagagttcgc ggagaggagg agcgccgcac agcccacggc ctcggcggtg
      721 cgcaccagga ccccgacgtt gcgcgggtcc tggatctcct cgcccgccag gatcacggtt
      781 tgcttccctc ccagcgattc gagggcgacc cggcgctgcc ggacgacgca gatcgccgtg
      841 acagccacct cgtagctggt gcccaccagc ttctggatca accctgcgct cgcgatgtac
      901 aggggaatat cttgtgccgc gcactcttcc gagaagctgc tggcttcgtc gcgggtggca
      961 aagacgctga gcacctccgc gggggatgca agcgcctggc ggacgaaatt ggccgtctcg
     1021 acgaggtaga gtccgctttg catccgctcg gcggggcgcg tcagcgagag agcgtgcttg
     1081 taggcagggt gcttgatgcc atcgatctct tccatagcca tacggagatt cgcgccccga
     1141 gagacgcgct cctgcggtgg atgaaaggtg gatcgcccgg caggaagagc gcgcacacgg
     1201 gattttgctc tctgattctc ttgacacgtt tggatgggat gccctataat aaccctgttg
     1261 cagagaggtg cgcgcattgg cgtgcgtggt tctgattcgg cgggtgcagg ctgctttcgc
     1321 cggcagagtg cgaacggtca agcgtgacgc ttgggaatgc tgggtgcgga tagcgagccg
     1381 ggagagtctt gagtgccctc gtagctcagt ggatagagct ggggcgtcct aagccctgag
     1441 tcgggggttc gaatccctcc gagggcgcca gcatctggac tcttcggtat caagggtcgc
     1501 agtcttctag cgttcacaac gttcgttccg cgtcaaaggt ccctccgcaa ctgcggacgg
     1561 gacttttttt gtgcactgca gcggatagct tccaccacgg cgttcccgcg atacgggagc
     1621 gtgtttctgt ccactgcggg cgcgcggcta ctgggtctcc accacactct ctgcagcacg
     1681 gaaggcaggc accgcagggg gtgtttgcct gggggatggc cgcccggtgc aacgaaatgg
     1741 catgggcctt gcctgggcag gtcatccgcg ggcctacatc aggcacctat gcacccacct
     1801 ctggatcccg ccagggatcc gagggggaag tcggacggcg atatgccgct tgagatgatc
     1861 ttaacgaaag ggtagagcaa tggcgaaggg tgacgagggc aagaagagca agaactcccg
     1921 catcggcgat gccaaggcga acatctgccg cgagtgtggc aagccgagca agccggtact
     1981 catgctcgat ggtggcaaga agcggctcgt gcttacctgc cacgagaagg tctgaacaga
     2041 agaccaccaa gcataagagg gaaggcggcg gatgcgccag agcgcacccc aacggccttc
     2101 cctctttcat ttaactgcgc ctttcaggcg aagactacca gtcgtcctcg cccatctcct
     2161 cccactccag atcctcgggc aagagcccga cgcgatcggg gtggcgtctc tcccattcct
     2221 ccttggccgc tcgcgccagc tcgtccgggt cgtgcatgga ggcggtgacc tccacatact
     2281 cccaccgctc acggcccagg tcatcggtgt agggtacctg tgctcgatac cggggcatcg
     2341 caagccgctc ctttcatgcc gccagaatcc cgcttctcct cccatctcgc acgacacagg
     2401 ccaatcgggc ttgggctggg attccgagat aaggaatccg aagcctcgga ggcagcacgc
     2461 aacccggcga gcggcgataa atcaccgcgc tgcttgcgag cgaagccctg ccgggctggg
     2521 ttggggatac tcccagatgt ctcaaaacgg cggtagctgg gaggggaagc cccgaggggg
     2581 ctttgccgga aagcagcgcg gagcttcgag
//
LOCUS       DAOSCV010000653         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25681_length_2610_cov_3.797652, whole genome shotgun sequence.
ACCESSION   DAOSCV010000653 DAOSCV010000000
VERSION     DAOSCV010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25681_length_2610_cov_3.797652"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..2030)
                     /locus_tag="PLY56_14565"
     CDS             complement(183..2030)
                     /locus_tag="PLY56_14565"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ22748.1"
                     /translation="MALLGWETYFDKTLGCWMGKNSGGTLGGPVEGRKEALALDWYPV
                     VQEGGIPNDDLEVQLVWLHALETRGIALTAEDLAAEWLDHYLPNWDEYGFSKTNVRKG
                     LLPPLSGAWNNWFSESMGCPIRSEIWACVAPGLPLLAARLAREDAIVDHTDESAYAEI
                     LFAVIESAAFVEEDRDRLMALGLAAIPDESRVARAVRVMQETWQRTGSWEATRQAILE
                     AEGRWNFTDAPQNIAFTLLGWYSSPNDFGQAICDSVNCGYDTDCTGATLGAILGTAWG
                     ARRLPERWMKPLGDSIAIDPRRTRLHAVPKTLQELAERTAAIAVAMLAQHGYAFGPGR
                     VLEVALPTPEELRQQMQDAGLWRNRRDEVERRFHHGRVRVRYPGGPVLAPGGKSQVTV
                     TVTNDTERRMEGPVAVAPLAPGLSARIAPEAVDLLPGESAELTIELSLDAGAPPRAHS
                     DAAIALLAEGIAPWSFTVSVIRPMYWQMERWPAEAYESTEDAAPIPGGERVAVEGNRM
                     PLPEPGMWVGRTRVLNPSAHERPVRICSPSNYPVRVWLNGEKVVENPGGPIRPSYHAN
                     PAHHYGAGILLPGWNELAAAWLRGDEPGEAHLFLTAPDGTGWGDLVFEP"
     gene            complement(2139..>2610)
                     /locus_tag="PLY56_14570"
     CDS             complement(2139..>2610)
                     /locus_tag="PLY56_14570"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamine-synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HOJ22749.1"
                     /translation="EDPSPEMADEVRAMKRRVEKERTDRTTRARDLKLGPGGMSDVEF
                     LVQWLQLLHAGRHPALREPSTLAALPVLVSLGLLPEDEAGILREGYRFQMRLRNLLYL
                     RFGRPVAVLPEGDEDQRALARALEMENAGELLEAFYAHRERVRAIFERRFYGDG"
BASE COUNT          425 a          943 c          837 g          405 t
ORIGIN      
        1 agccggcacg cgcgtcgcgc tgcccgccag gcacacggcc acgaacaaga gcccccaggc
       61 catccagagc gggctcgaaa cccggatcct ctgtgtcaac accttcatcc ctcccccgcc
      121 ggcggcgtgc cgccaggcat tatcatcctt atcatccgga agtagccacg catcttcatg
      181 gactacggct cgaagacgag gtctccccat ccggtaccgt caggcgcagt gaggaagagg
      241 tgcgcctcgc ccggctcgtc gccgcgcagc catgccgccg ccagctcgtt ccaccctggc
      301 agcaggattc ccgccccgta gtggtgcgcc ggattggcat gatagctcgg gcgaatcggc
      361 ccgcccgggt tctccaccac cttctcacca ttcagccaga cgcggaccgg gtagttggac
      421 ggcgagcaga tccgcaccgg gcgctcgtgc gccgaagggt tcagcacgcg cgtccggcct
      481 acccacatcc ccggttccgg aagcggcatc cggtttccct cgaccgccac gcgctctccg
      541 cccggaatgg gagccgcgtc ctcggtgctc tcatacgcct cggctggcca gcgttccatc
      601 tgccaataca tcgggcgaat gaccgatacc gtgaagctcc agggggcaat cccctcggcc
      661 aggagggcaa tggccgcgtc actatgggcg cgcggaggcg ccccggcatc gagtgacaac
      721 tcgatggtaa gctccgcgct ctcgccggga agcaggtcga ccgcctcggg cgcgatcctc
      781 gccgaaagcc cgggcgcgag aggcgccacg gcgacaggcc cctccatgcg ccgctcggtg
      841 tcgttcgtca cggtgactgt cacctggctc ttgccgcccg gggcgagcac gggcccgcca
      901 gggtatcgga cgcggacgcg cccatgatgg aagcggcgct ccacctcatc gcggcggttc
      961 cgccacaggc cggcatcctg catctgctgg cgcagctcct ccggcgtggg aagcgcaacc
     1021 tcgagcacgc gcccggggcc gaaggcgtag ccgtgttgcg ccagcatcgc cacggcgata
     1081 gccgccgtgc gctccgcgag ctcctgaagt gttttcggca ccgcgtgcag gcgcgtgcgc
     1141 cggggatcga tggcgatgga atcgccgagt ggcttcatcc accgctctgg gaggcggcgc
     1201 gccccccagg ccgtgccgag gatggcgccc agcgtcgcgc cggtgcagtc ggtgtcatag
     1261 ccgcagttga ccgaatcgca gatcgcctgg ccgaagtcat tgggcgacga gtaccatccc
     1321 aggagtgtga acgcgatatt ctggggcgcg tcggtgaagt tccagcggcc ctctgcctcc
     1381 aggattgcct ggcgagtggc ctcccagctc cccgtccgct gccacgtctc ctgcatcacg
     1441 cgcacggcgc gcgcgacgcg cgattcatcc gggatcgcgg cgagccccaa cgccatcagc
     1501 cggtcgcgat cctcctcgac aaacgcggcg ctttcaatga cggcgaagag gatctcggcg
     1561 taggccgatt catcggtgtg gtcaacgatg gcgtcctcgc gggcgagacg ggcggcgagc
     1621 agcggcaggc cgggagcgac gcacgcccag atctccgagc ggatggggca ccccatgctc
     1681 tcggaaaacc agttgttcca cgcgccggaa agagggggca gcagaccctt gcgcacgttg
     1741 gtcttgctga agccatactc gtcccagttg ggcaggtagt gatccagcca ctccgcggcg
     1801 agatcctcgg cggtgagggc gattcccctg gtttcgaggg catggagcca gacgagctgc
     1861 acttccaggt catcgttggg gatgccgcct tcctggacca cgggatacca atcgagcgcc
     1921 agcgcctcct tgcgcccttc aaccggcccg cccagggtgc ctccagagtt cttccccatc
     1981 cagcatccca gagttttatc gaaataggtc tcccaaccga gcagcgccat ccaatcctcc
     2041 cgaaggcccc gaacacgctc tttgtcaagg cgcgggcgtt ttccaaaccc aacgtttcgc
     2101 ggacaaaccg cgcgttcctg cccggcagga cggcgggcct acccgtcgcc gtagaaccga
     2161 cgctcgaaga tggcgcgcac ccgctcgcga tgggcgtaga acgcctcgag cagctcgccc
     2221 gcgttctcca tttcaagggc acgcgccagc gcccgctggt cttcatcccc ctcgggaagc
     2281 accgcaaccg ggcgtccgaa acgcaggtag aggaggttgc gcaggcgcat ctggaagcgg
     2341 tagccctcgc gcaggatgcc ggcctcgtcc tccgggagca gaccgagcga gacgagcact
     2401 ggcagcgccg cgagcgtgct cggctcgcgc agcgccgggt gccgcccggc gtggaggagc
     2461 tgcagccatt gcaccaggaa ctccacgtcc gacatcccgc ctgggcccag cttgagatcg
     2521 cgcgcccggg tggtgcgatc cgtgcgctcc ttctcaacgc gccgcttcat ggcgcgcact
     2581 tcatcggcca tctccggcga gggatcctcc
//
LOCUS       DAOSCV010000654         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25726_length_2607_cov_3.685345, whole genome shotgun sequence.
ACCESSION   DAOSCV010000654 DAOSCV010000000
VERSION     DAOSCV010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25726_length_2607_cov_3.685345"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLY56_14575"
     CDS             <1..571
                     /locus_tag="PLY56_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22750.1"
                     /translation="GGARILTGDTEGTPVAAAWSPDGSGVACLFVSDDGGEERRSLRV
                     YRVGEDEPATRPEIPPGCVEIAWDAAGEGLFCRVAGEDPGLWYVAATEGSQPRRVYAG
                     RVLQFASLAGGHALLVVPAREKPWLHQLYLAGIAGEEARRVTNTPSFDHLDSVSPDYR
                     SVLITRRPRDDRQSLFPEATLWRFDLRGE"
     gene            complement(650..1615)
                     /locus_tag="PLY56_14580"
     CDS             complement(650..1615)
                     /locus_tag="PLY56_14580"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ22751.1"
                     /translation="MHLLQGGRLAEGIPSPTAVAIGMFDGVHCGHQALIRRAMAIASD
                     RGLVPLVLTLDRHPDEVLRPDRARPYLTTLPEKLALIRALGISLVVVARVTPAFLSIQ
                     ARDFVMRSLRGELDARAVIVGRNFRFGQGRQGDIALLTAMQKECGFTLEAVDPITADG
                     EPISSTLIRRVLAAGEVAHARRLLGRPYQMAGEVVSGEKIGRSLGFPTANIAYPPQKV
                     LPALGVYVCQACVEGACYGAAVNVGRRPTFDGTRITVEAHLLGFEGDLYGKQLNLRFL
                     DRLRTEEQFPTPEALRAQIAADVAQAHELYASLAASLETCAQSQT"
     gene            1772..>2607
                     /locus_tag="PLY56_14585"
     CDS             1772..>2607
                     /locus_tag="PLY56_14585"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ22752.1"
                     /translation="MSQARPSDVAERMPQNTILAAIAAGHRLSLLSDLVCPACGREML
                     TEVASQGASPTSLACNHCGRRWPVRQRTPAFLPPVETAIPSGAEALLHAPDVERWDAF
                     CHAVRDADAALGAEVFDFSRDDWRFLLPLPPDAQVLLLGCGWGRLASSLADIAGRVYA
                     VDQDEAKTRFVNARAFAEGRSNLLGITAGIHALPFRRASFHLVVVADPTVIEQKVTGR
                     SAVTNLVCLRAFLAQNGVLLLRASRGVPAGMLAVDAWRWERRLRHAGFKRIEDLLALP
                     DA"
BASE COUNT          410 a          858 c          904 g          435 t
ORIGIN      
        1 ggggggcgcg cggattctga ccggcgacac ggagggcacg ccagtcgcgg cggcgtggtc
       61 tcccgacggg agtggtgtcg cctgcctctt tgtctcggac gatgggggag aggagcgtcg
      121 gagcttgcgg gtgtaccggg tgggagagga tgagcccgca acgcgccccg agattccccc
      181 ggggtgcgtg gagatcgcct gggatgccgc cggcgagggc ctcttctgcc gtgtcgcggg
      241 ggaggatcct ggactgtggt acgtggccgc aaccgagggg agccagccgc gccgcgtcta
      301 tgcgggccgg gtgctccagt tcgcgtcact tgccggcggt cacgcgctcc tcgtcgttcc
      361 cgccagggag aagccgtggc tccaccagct ctatctcgcg ggcatcgccg gggaggaggc
      421 acgacgcgtc accaacactc ccagctttga ccacctcgat tccgtgtcgc ccgactaccg
      481 aagcgtgctc atcacgcgcc ggccgcgcga cgaccggcag tcgctcttcc ccgaggcaac
      541 tctttggcgg ttcgatctgc ggggggagtg atgcggccgg gcgcgccggg caggcgggac
      601 gctgccagga aggacgcgcc cttgcgaggc tccactggag gcgttccctc tacgtctggg
      661 actgcgcgca tgtctcgagg gatgccgcga gcgacgcgta gagttcgtgc gcttgcgcga
      721 catcagcggc gatctgcgcg cgcaacgcct cgggggtggg gaactgctcc tcggtgcgaa
      781 gccggtcgag gaagcgcagg ttcagctgct tgccgtagag gtcgccctcg aaccccagga
      841 ggtgcgcctc cacggtgatc cgcgtgccat cgaaggtggg gcgccggccg acattcacgg
      901 cggcgccgta gcaggcgcct tccacacagg cctgacacac atacaccccg agcgcgggca
      961 ggaccttttg aggcggatag gcgatgttgg ccgtggggaa gcccaggctg cgtccgatct
     1021 tttcgccgga gaccacctcg cccgccatct ggtaggggcg ccccagcagg cggcgagcat
     1081 gtgccacctc gcccgctgca aggacgcgcc ggattagcgt gctactgatc ggctcgccat
     1141 cggcagtgat cggatcgacc gcctccagcg tgaagccgca ctccttctgc atcgcggtca
     1201 ggagagcgat atcgccctgg cgcccttgcc cgaaccggaa gttgcggccc acgataaccg
     1261 cacgcgcgtc caactcaccg cgaagcgagc gcatcacaaa gtcgcgcgcc tgaatcgaga
     1321 ggaaagcggg cgtgacgcgc gcgaccacca cgagcgagat ccccagcgcg cggatcaggg
     1381 cgagcttctc cgggagcgtg gtcaggtagg gccttgcccg gtcgggccgc aggacctcat
     1441 ccgggtgccg gtcgagtgtg agtacgagag gtaccaaacc ccgatcgctg gcaatggcca
     1501 tcgcccggcg gatcagcgcc tggtgaccac aatgaacgcc atcaaacatt cctatcgcga
     1561 ccgccgtggg agaagggatt ccctctgcca ggcgtccacc ttgcagtaag tgcatcggtt
     1621 tgattgctcc atccgcactc cagtttcgcc gcgctctgtt ccaatgcctg cccctgaagc
     1681 gcctcgatcc tctctggcat cgcttgtgca aacccggcca aaataccagg aggctggacg
     1741 agcagggggg cggtcaaggg atggtgacac catgagccaa gcgcgaccat cagacgtcgc
     1801 tgaacgcatg ccgcagaaca ccattctggc ggcaatcgct gcgggccatc gcctctcgct
     1861 cctgagcgac ctggtgtgtc cggcatgcgg gcgcgagatg ctgacggagg ttgcctccca
     1921 aggggcgagc cccacgagcc tggcgtgcaa ccactgcggc cggagatggc cggtccgcca
     1981 gcggacgccc gcgttcctgc cgcccgtgga gacggcgatc ccttcgggtg ccgaggcgct
     2041 cctccacgcg ccggatgtgg agcgttggga tgccttctgc cacgccgttc gagacgcgga
     2101 cgcggcgttg ggcgccgagg tgttcgattt cagccgagac gattggcgct tcctgctgcc
     2161 tctcccgccc gatgcgcagg tgcttcttct ggggtgcgga tgggggcgcc tggcatcctc
     2221 gctggccgat atcgccgggc gtgtctatgc tgtggaccaa gacgaggcca agacacgctt
     2281 tgtcaatgcc cgcgcctttg cggagggccg gtcaaacctt ctgggaatca ccgccgggat
     2341 ccacgcgctt cctttccgcc gcgccagctt ccacctggtc gttgttgccg acccaacggt
     2401 catcgagcag aaagtgaccg gccggagcgc agtaaccaac ctggtatgtc tgcgcgcctt
     2461 tctggcccaa aacggcgtgt tgttgttgcg ggccagcaga ggggtgccgg cgggcatgtt
     2521 agcggtagat gcctggcggt gggaacgccg cttgcggcac gcgggcttca agcggatcga
     2581 ggatctcctg gcgcttcccg atgccga
//
LOCUS       DAOSCV010000655         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25740_length_2606_cov_3.657389, whole genome shotgun sequence.
ACCESSION   DAOSCV010000655 DAOSCV010000000
VERSION     DAOSCV010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25740_length_2606_cov_3.657389"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            353..1741
                     /locus_tag="PLY56_14590"
     CDS             353..1741
                     /locus_tag="PLY56_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ22753.1"
                     /translation="MELIKPKIVPPLDSDFRPAALANRSFREGVKASGQGVPLAIALE
                     RRDGTRSRYDTEVFAPGSPRAAENLAYVERIVKFLLWQRGGYKVIIGGPREIGEHIAK
                     VYSPQGERAFDYDFMGRQVYEKEFVVEITDADKVPPASEMSQALGRHLDGCRIGFDAG
                     ASDRKVSAVIDGQPVLSEEVIWNPRPQTNPDYHYHEIMSALHRAAAQMPRVDAIGVSS
                     AGVYINNRVMVASLFRGVPRDLFEAKVKDLFLRIRDEWGVPLEVVNDGEVTALAGSMS
                     LGVNSILGVAMGSSEAAGYVTPEGTITNWLNELAFAPVDYAPTAPVDEWSGDRGVGAL
                     YFSQQAIFRLAPVAGITLDENLGLAERLACFQELHKNGDPRTRPVYETIGIYLGYTMA
                     HYADFYDLEHALILGRVTSGDGGQIIIDKAYEVLRAEFPQLAERLNVTMPDEKAKRVG
                     QSIAAASLPELK"
     gene            1853..>2606
                     /locus_tag="PLY56_14595"
     CDS             1853..>2606
                     /locus_tag="PLY56_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ22754.1"
                     /translation="MKLRNSTAEIFVPDGKPVAEALARTTHMGVSAHQDDLEIMAFRG
                     ILECFGVDDKWFLGVVVTNGAGSPRDDLYKDYTDEEMMVVRRKEQKKAAFVGEYAAQF
                     LLDHPSSAVKDASNPDVVADLKEILLAARPEVVYTHNLGDKHDTHVGTALRLIQAMRE
                     LPEEARPKRVLGCEVWRDLDWMTDEDKVPLDVAAHENLAAALLGVFDSQICGGKRYDL
                     ATLGRRRAHATYHASHGTDDTTQLNFAMDLTPL"
BASE COUNT          458 a          831 c          880 g          437 t
ORIGIN      
        1 gccaaagaac aggacgtgcg cctcgccctg ggctcggatg cccaccaccg gtagcacctg
       61 tcgctcccac cgctatgggg ggctatacgg cccgccgcgc ctggcatggg ccggcggatg
      121 tgctcaacat gctctcgctc gaggagcggt tgagccgctt gagaaagtgg tgcgtgaagc
      181 atgaaccgca aggcgtgatc cgcaaggcgt aaggccctgt gccgtcggag gcggtcttct
      241 gcagcccctc gcgacgggct gacttcggcc tgccgtggct ttacggttta cggattgcgg
      301 gttgcggatc tcggctggcg catcatgttt tggatgaagg atgggaagac gcatggaact
      361 catcaaaccg aagattgtcc cgcctcttga ttctgatttc cgcccggcgg ctctcgccaa
      421 tcgctcattc cgggagggtg tgaaggcctc cggtcagggc gttcccctgg cgatcgccct
      481 ggagcgcaga gacggcacgc gctcccgcta cgacaccgag gtcttcgcgc ccggctcgcc
      541 gcgcgccgcg gagaacctcg cctatgtcga gcggatcgtc aagttcctgc tgtggcagcg
      601 cggcggctac aaggtgatca ttggcggccc gcgcgagatc ggcgagcaca tcgccaaggt
      661 ctactcgccg cagggcgagc gtgctttcga ctacgacttc atgggccggc aggtctatga
      721 gaaggagttc gtcgtcgaga ttacggatgc cgacaaggtg ccgccggcct ccgagatgag
      781 ccaggcgctg ggccgccacc tggatgggtg tcgcatcggc ttcgacgcgg gcgccagtga
      841 ccgcaaggtc tccgcggtga tcgacggcca gccggtcctc agcgaggaag tcatctggaa
      901 cccccgcccg cagacgaacc cggattacca ctatcatgag atcatgagcg cgctgcaccg
      961 cgcggcggcg cagatgccgc gcgtcgatgc catcggcgtc agctccgcgg gcgtctacat
     1021 caacaaccgc gttatggtgg cttcgctctt ccgtggcgtg ccccgggatc tcttcgaggc
     1081 caaggtgaag gacctcttcc tgcgcatccg cgacgagtgg ggcgtgccgc tcgaagtggt
     1141 caacgatgga gaagtgaccg cgctcgctgg ctcgatgagc ctgggcgtca actccatcct
     1201 cggcgtggcg atgggctcca gcgaggctgc cggctacgtc acgccggaag gcaccatcac
     1261 caactggttg aacgagctgg cgttcgcgcc cgtggattat gccccgacgg cccccgtgga
     1321 tgagtggtcg ggcgaccgcg gcgtgggcgc gctctacttc tcgcagcagg cgatcttccg
     1381 gctggcgccc gtggccggca tcacgctcga tgaaaacctg ggccttgccg agcgcctggc
     1441 gtgcttccag gagctgcaca agaacggcga tccgcgcacg cgcccggtgt acgagacgat
     1501 cggcatctat ctgggctaca cgatggcgca ctatgccgac ttctatgatc tggagcatgc
     1561 gctcatcctc ggccgggtca cctcgggcga cggaggccag atcatcatcg acaaggcgta
     1621 tgaggtcctg cgggcagagt tcccgcagct ggcggagcgc ctcaacgtga cgatgccgga
     1681 cgagaaggcg aagcgggtcg gacagtcgat tgcagccgcg agcctgccgg aactgaagta
     1741 aaaacgtagg gcgcgagatg gaaggggtgg tcccgcgcgc gagctatcgc gcaggctgac
     1801 agggcatgcc gcttccgttt cgcgccctct acactgcact taggagaata aaatgaagct
     1861 gagaaacagt actgccgaga tcttcgtgcc ggacggcaag ccggttgccg aagcgctggc
     1921 gcgcacgacg cacatgggcg tttcggccca tcaggatgac ctggagatca tggcgttccg
     1981 gggcatcctg gagtgctttg gcgtggacga caagtggttc ctgggcgtgg tggtcaccaa
     2041 tggcgccggc agcccgcgcg acgacctgta caaggactac accgacgagg agatgatggt
     2101 cgttcgccgc aaggagcaga agaaggcggc cttcgtcggc gagtacgccg cgcagttcct
     2161 gctggatcac ccgagctctg ccgtgaagga cgcgagcaac ccggatgtgg tggccgacct
     2221 gaaggagatc ctcctcgccg cgcgcccgga agtggtctac acccataacc tgggtgacaa
     2281 gcacgacacc cacgtcggca cggcgctgcg cctcatccag gcgatgcgcg agctgcctga
     2341 ggaagcccgg ccgaagcgcg tcctcggctg tgaggtgtgg cgcgacctcg attggatgac
     2401 cgacgaggat aaggtgcccc tcgacgtggc ggcgcacgag aacctggcgg ctgcgctcct
     2461 gggcgtcttc gactcccaga tctgcggcgg caagcggtat gatctggcga cgctgggccg
     2521 ccgccgcgcc cacgccacct accacgcgtc gcacggcacc gacgacacca cccagctcaa
     2581 cttcgccatg gacctgacgc cgctgg
//
LOCUS       DAOSCV010000656         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25771_length_2604_cov_2.576697, whole genome shotgun sequence.
ACCESSION   DAOSCV010000656 DAOSCV010000000
VERSION     DAOSCV010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25771_length_2604_cov_2.576697"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLY56_14600"
     CDS             <1..271
                     /locus_tag="PLY56_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOJ22755.1"
                     /translation="VQKAVRDVIEAKKVAETRTPYRSRRVQITDTMPLDEIMITLTEL
                     EKEMKEAARALEFEQAAELRDEIARIKKLLPGSGGGRKPALSKRR"
     assembly_gap    537..546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <547..1916
                     /locus_tag="PLY56_14605"
     CDS             <547..1916
                     /locus_tag="PLY56_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ22756.1"
                     /translation="PTGSGKTLAAFLWGIDRLFREKSTDAAAPARRGRGIKPRGVGIL
                     YISPLKALNNDVERNLRIPLEGIRETAESLGVTLPELRVGVRSGDTHQRERQAMVRRP
                     PDILITTPESLYLLLTSPTARDILRSVHTVIVDEIHTLVGTKRGVHLALSLERLQHLA
                     GAPIQRIGLSATIRPLEEAARFLGGCEWDPEPGSPGEVVVKEGPLNAPWPTPRPVTVV
                     DAHYRKELDLRVLSPVADHRHLPGGSLWPTLIPHVADLIEEHRSTLIFCNSRRMAERT
                     ADRLNVEAAAREEGQQLALLQDGAARGVGFFAAGDGTIPTPIRAHHGSVSREARLEME
                     QALKAGELPALVATSSLELGIDIGSVDLVIQLQAPEGVARGLQRAGRAGHRVGQTSKA
                     LIFPTHAEDLIAAAAVAGGMLKGEVETTTTPRNPLDVLAQQIVAMVSVETWNADALYH
                     LVRRA"
     gene            1962..>2604
                     /locus_tag="PLY56_14610"
     CDS             1962..>2604
                     /locus_tag="PLY56_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ22757.1"
                     /translation="MLAGRYPTDANRGLRPRLSWDRVNNTLAGLPGSRSIAIANGGTI
                     PDTGTYTLLLGDGATRLGELDEEFVHETRVGDTFMLGSQVWRVVDITDDKVSVAPAPG
                     VIPRMPFWRGEAPWRPYELGVRVGEFRRLVAERLRGLEGAWPSSPETPEIWKRPEIAS
                     VLEWLRASYALDENSARSVVSYVARQLRAAGAISSDRCIVVESFRDALGDPRVV"
BASE COUNT          405 a          944 c          867 g          378 t
ORIGIN      
        1 ggtccagaag gcggtgcgcg acgtgatcga ggcgaagaag gtcgccgaga cgcgtacccc
       61 ctaccggtcg cgacgcgtgc agatcacgga cacgatgccg ctcgacgaga tcatgatcac
      121 cctgacggag ctggagaagg agatgaaaga ggcggctcgg gcgctggagt tcgagcaggc
      181 cgccgagctg cgcgatgaga tcgcgcgcat caagaagctc cttccgggct ccggcggcgg
      241 gcgcaagccc gccctctcga aacggcgctg agccacccca ccgcgagaag tcccaccggg
      301 ctggagtgca aggaactcgt ttcgagggtg gcagccggct cccgcaaccg cttgcctggc
      361 atccccaggt gtgctatgat ccgggcagag aggtgcgttc atgtcacagc cccatcccga
      421 ggtcctcgac gcctttagcc tgcccgtacg cgcctggttc gagaaggcgc tcggctcgcc
      481 cactccgccc caggcccagg ggtgggcggc gatccagcgc ggcgtgccca tccccannnn
      541 nnnnnncgcc caccggctcg ggcaagacgc tggccgcgtt cctatggggg atcgaccggc
      601 tctttcgcga gaagagcaca gacgcagccg ccccggcacg ccgcgggcgt ggcataaagc
      661 cgcgcggtgt cggcatcctc tacatctcgc cactcaaggc gttgaacaat gacgtcgagc
      721 gcaacctacg catccccctg gaggggattc gcgagaccgc ggaatcactc ggcgtaacgc
      781 tccccgagct ccgggtcggg gtgcgctctg gcgacacgca ccagcgggag cgccaggcga
      841 tggtccgccg gccccccgat atcctgatca ccactcccga gtcgctctac ctcctcctga
      901 ccagccccac cgcccgcgac atcctgcgga gcgtccacac cgtgatcgtc gatgagatcc
      961 acacgctcgt cggcaccaag cgcggcgtcc acctcgccct cagtctcgag cggctgcaac
     1021 acctggccgg ggctccgatc cagcgcatcg gcctctcagc cacgatccgc ccgctggaag
     1081 aggccgcgcg cttcctcggc gggtgcgagt gggatcccga gccgggcagc ccaggcgagg
     1141 tcgtcgttaa ggaagggcca ctgaacgccc catggcccac gccgcggccc gtgaccgtgg
     1201 tggatgccca ctatcggaag gagctcgacc tgcgcgtgct ctcgcccgtc gccgaccacc
     1261 gccacttgcc gggcggctcc ctctggccga cgctcatccc gcatgtggcg gatctgatcg
     1321 aggagcaccg gagcacgctc atcttctgca acagccggcg catggccgag cgcactgccg
     1381 accggctcaa cgtggaggcc gccgcccgcg aggaggggca acagctggca cttctccagg
     1441 atggcgccgc ccgcggtgtt ggcttcttcg ccgctggcga tggcactatc ccgaccccga
     1501 tccgcgcgca ccacggcagc gtctcgcgcg aggcccggct ggagatggag caggcactca
     1561 aagccggtga gcttccggcg ctggtggcca cctcgtcgct ggagctgggg atcgatatcg
     1621 gctccgtgga tctcgtcatc cagcttcagg cccccgaggg cgtggcgcgc gggctgcagc
     1681 gcgccgggcg cgcgggccac cgcgtcggcc aaacttccaa ggcgctcatc ttccccaccc
     1741 atgccgagga cctcatcgcc gccgccgcgg tcgccggcgg gatgctaaag ggcgaagtgg
     1801 agacgaccac cactccgcgg aatcccctcg acgtcctcgc gcaacagatc gtggcgatgg
     1861 tctccgtgga gacgtggaac gcggacgcgc tctaccatct ggtgcgccgc gcctagccct
     1921 accaggatct ctcccgaccc gccttcgacg ccgtcctgga gatgcttgcc gggcggtatc
     1981 ccaccgacgc caaccggggg ctgcgcccac gcctctcgtg ggaccgcgtg aacaacacgc
     2041 tggccgggct ccccggatcg cgctccatcg cgatcgccaa cggcggcacc atccccgaca
     2101 cgggcaccta cacgctcctc ctcggcgacg gcgcgacccg gttgggcgag ctggacgagg
     2161 agtttgttca cgagacgcgc gtcggcgaca ccttcatgct cgggtcgcag gtgtggcgcg
     2221 tcgtcgacat cacggatgac aaggtgagcg tggcgccggc gcccggcgtg atcccgcgca
     2281 tgccgttctg gaggggcgag gcgccctggc gcccttacga gctgggggtg cgcgtcggtg
     2341 agttccgccg gctcgttgcc gagcgcctgc gcggcctgga gggagcatgg ccgtcctctc
     2401 cggagacccc ggagatctgg aaaagacccg agatcgcgag cgtcctggag tggctgcgcg
     2461 ccagctacgc cctcgatgag aactccgcgc ggagcgtggt gagttacgtc gcccggcagc
     2521 ttcgcgccgc cggcgccatc tcctccgacc ggtgcatcgt ggtcgagtcg ttccgcgacg
     2581 cgctaggcga cccgcgtgtc gtga
//
LOCUS       DAOSCV010000657         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_25830_length_2599_cov_4.558962, whole genome shotgun sequence.
ACCESSION   DAOSCV010000657 DAOSCV010000000
VERSION     DAOSCV010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25830_length_2599_cov_4.558962"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1819)
                     /locus_tag="PLY56_14615"
     CDS             complement(<1..1819)
                     /locus_tag="PLY56_14615"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ22758.1"
                     /translation="MRRVVGALLLCLFWGSAAWGLDVVRDGKPVARIVVPDEADSYTT
                     RAAGWIRDYVKKATGAELAVVPEGQDTGQGPRIALGATKAARQARVRADDLAWDGCRL
                     VVKGDTLFLVGRDEDGVGKADYKAPKGTCRAATAFLEEFCGVRWLAPTPLGEVVPQRR
                     DISVPDDLNRVIVPAFGYANGRLIYGVRTPASYANNFREALRLYTAGGHTWPVFVPLE
                     RYYEEHPEYFALINGQRSNSRMNHLCTTNPDVKRLLLEGLRGKFDEGYDWAQLAQSDG
                     YRRCECPNCEAMDNYREWGRGDAFLFKTLAENPCERVLKLHRDIAEQCRRSHPNKKVH
                     ILSYGPTTMPSRAFDKFPDNVVVELCNTDPRVLAAWRGKAKAFTAYVYYYGTYNAPGP
                     GPKFTAAQAAEELRRLRDHQIQGIYFCGIGECWGLEGHSYYACAKLLGNPDLDPQALV
                     AEYCNGLYGKAAPAMLRFYEALDSVVSLYYPMKRLSGGRLASLNTAEAAYIAMYPPAV
                     LSRLESLLRSAEQLADDERSRKWIGLTRISFDYVRLTATAYTLYRAYQASPNRAALLQ
                     LQDAVEKWRAHRDRILNLDKKSVAEWFPGHASWVDFFKTK"
     gene            complement(1948..2403)
                     /locus_tag="PLY56_14620"
     CDS             complement(1948..2403)
                     /locus_tag="PLY56_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOJ22759.1"
                     /translation="MRVYRLDLTVPDEAVDENAHVNNVEYIRWMEEAARAHAAHQGST
                     AAAQAIGATWYARAIRVEYLRPAFAGEHLAILTWVADFHKVRSLRRYRIFRVTDGVLL
                     AEGETDWVFVDAATGRPRSIPPEVTRLFEVVPEDEAPQSLAGVAPARSL"
BASE COUNT          459 a          893 c          813 g          434 t
ORIGIN      
        1 ctttggtctt gaagaaatcg acccaggagg catggccggg gaaccactcg gcgaccgatt
       61 tcttatccag gttgaggatt cggtcgcggt gggcacgcca tttctccacc gcgtcctgga
      121 gttggagaag cgcggcgcgg ttggggctcg cctggtaggc gcggtagagg gtgtaggctg
      181 ttgccgtcag ccgcacgtag tcgaagctga tgcgcgtcag cccaatccac ttccggctgc
      241 gctcgtcgtc ggcgagttgc tcggcactgc gcagcaggct ctccaggcgg ctgaggacag
      301 ccggggggta catcgcgatg taggctgcct ccgccgtatt caacgaggcc agccggccgc
      361 cagagagacg cttcatgggg tagtagaggg agaccacgct atccagcgcc tcgtagaaac
      421 gaagcatcgc cggtgcagcc ttgccgtaga gaccgttgca atactcggcg acgagcgcct
      481 gcgggtcgag atccggattg cccagaagct tcgcgcacgc gtagtaggag tggccttcca
      541 gcccccagca ctcaccgatg ccgcagaagt agatcccctg gatctggtga tcgcgcagcc
      601 ggcgcagctc ctccgcggcc tgtgccgcgg taaacttcgg ccctggcccg ggagcgttgt
      661 aagtaccata gtagtacaca tacgctgtaa aggccttcgc tttgcccctc cacgccgcga
      721 gcacgcgcgg atccgtattg cacagctcga ccacgacgtt gtccgggaac ttatcgaacg
      781 cgcgcgaggg catggtggtc ggcccataag agaggatatg caccttctta ttgggatgcg
      841 accggcggca ctgctcggca atatcgcgat gcagcttcag gacgcgctcg cacgggttct
      901 cggccagcgt cttgaagaga aacgcatcgc cacgccccca ctcgcgatag ttgtccatcg
      961 cctcgcagtt cgggcactcg caccggcggt agccatccga ttgcgccagc tgcgcccagt
     1021 catagccctc atcgaacttg ccgcgcaggc cctctaggag gaggcgcttc acgtcggggt
     1081 tcgtggtgca gaggtggttc atgcgactgt tactgcgctg tccattgatg agagcgaagt
     1141 actccgggtg ctcctcgtag tagcgctcca gcgggacgaa aacaggccag gtgtgcccgc
     1201 cggccgtgta gaggcggagc gcctcgcgga agttgttggc gtaggaggca ggagtcctca
     1261 caccgtagat cagccgccca ttggcgtagc caaacgccgg cacaatcacc cggttcaggt
     1321 catcaggaac cgagatgtcg cgccgttgcg gcaccacctc cccgagcggg gtgggcgcca
     1381 gccagcgcac gccgcaaaac tcttccagaa acgcggtggc cgcgcggcag gtgcctttgg
     1441 gcgccttgta atccgcctta cccacgccat cctcatcgcg gcccaccaga aagagggtat
     1501 cgcccttcac cacgaggcgg cagccgtccc aggcaagatc atcggcgcga acgcgcgcct
     1561 ggcgcgccgc cttcgtggcg ccgagagcga tccgcggccc ctggcccgta tcctggcctt
     1621 ccgggaccac agcaagctcc gcgcccgtcg ccttcttcac gtagtcgcga atccaacccg
     1681 ccgcccgggt cgtgtagctg tcggcctcat ccggcaccac gatccgcgcg accggcttcc
     1741 cgtcccggac cacgtcgagg ccccacgcgg cggagcccca gaacaggcac agcagcaacg
     1801 caccgacaac tcgtctcatc gctccctcct tcggacggcc cacctatcta ccgcctgttg
     1861 ggaacccgct tcgacatccg gccgggcaat gccttcctcg cgcacggtgc accgttccca
     1921 tacgaaaagg ggtatgcggc aacgagttca gagagagcgt gctggcgcca cgccggccag
     1981 actctgggga gcctcatcct ctggaaccac ctcgaagagg cgcgtcacct caggcggaat
     2041 cgaccgcggg cgtccggtcg cggcatcgac gaagacccaa tccgtctcgc cttcggcgag
     2101 gaggacgcca tcggtaaccc gaaagatgcg atagcggcgc agcgagcgca ccttgtgaaa
     2161 gtcggccacc caggtgagga tggccaggtg ctcgccggca aaggcgggtc gcaagtactc
     2221 cacccggatc gcgcgcgcgt accacgtggc gccgatggcc tgagcggccg ccgtgcttcc
     2281 ctgatgcgcc gcgtgggcac gcgccgcctc ctccatccag cggatatact ctacgttgtt
     2341 gacgtgcgca ttctcatcca ccgcctcgtc gggcaccgtc aggtccaacc ggtacactcg
     2401 cacggccaca ctcctctctt gccctggata gatgccaccg cgttcgctcg cgcgctggcc
     2461 ctggcgccat tataccgtac gccaggcaat gcctcaaccg ccgcagggcg cagcctggga
     2521 ttccgacaca aggggaaggc cgcccgcacg cgcggagctc gaagctccgc gctgctttcc
     2581 ggcaaagccc ttcggggct
//
LOCUS       DAOSCV010000658         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26027_length_2584_cov_4.023725, whole genome shotgun sequence.
ACCESSION   DAOSCV010000658 DAOSCV010000000
VERSION     DAOSCV010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26027_length_2584_cov_4.023725"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(563..2215)
                     /locus_tag="PLY56_14625"
     CDS             complement(563..2215)
                     /locus_tag="PLY56_14625"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOJ22760.1"
                     /translation="MDCQISALQKARASYQPKLPPALRAGNVKVVLGAPTESASDQEE
                     IKKLFPHTYGQPVATLAEGDGSLSTAPLKVGVVLSGGQAPGGHNVIAGLFDALKAANP
                     ESRLFGFLKGPGGIIKGKYKELTSDVIDRYRNTGGFDMIMSGRDKIEKPEDLAACKAN
                     LEQMGLDGLVIVGGDDSNTNAALLAEYLRAQDCPTCVIGVPKTIDGDMKNAQIETSFG
                     FDTAAKTYSELIGNIERDATSAVKYWHFIRLMGRAASHVTLECALQTHPNITLISEEV
                     QAKGTTLAEIVDYIVDVVVKRAAAGKNYGVLLVPEGLVEFIPDIKVMIDELSTILGRD
                     EQYLRSLPDHSERAQYLNTQLTAESAQVYNSLPVAIQEVLLTRDSHGNVPLSQVETER
                     LLIDLVSDKIRLMKARGETEAKFSPLSHFFGYEGRCAAPSNFDADYTYSLGYAAAHLI
                     RGGLTGYTVNVQNVTKPVDQWVAGGTPVTMMLNLEKRKGKMVPVIKKALVDLDGKPFK
                     VFAENRDKWALEDHYIFPGPIQYFGPAEVCDAPTMTLALEKK"
BASE COUNT          457 a          819 c          828 g          480 t
ORIGIN      
        1 ggcccccagg cgcgggttgc actgcgggca gttgctagga ccgcattcat agcagccaag
       61 gctcagtcgc ctaaagagcg ccttggcgat tatccacacc aaccgccagg tgtacccgca
      121 gtgatagcag atcaccgacg catctccgga agaaccatca agcattggat ccccctgtgt
      181 gccggcctat tatcgcacgt gaggaggaac ccgtcaagcg cccgccggcg ccgcgaagag
      241 cagccggctc cacaggccca gagcaggtag agcgcgcccg gatagagaga ggaccacgcc
      301 ctcccccata ggccggcgcc tgcccctcga cgcggcggca atctagttgt tcggcatcgg
      361 agccgcttct cccttcgaga agcgggctat caggcgctct tctcccgcga ggagcacgat
      421 acgcgggcgc agcggcgaga gcttgagagg ggcgttgcgt gtctggcggg caaagcaggg
      481 cccggcatgc gagtgtgaag aggggatatg gacggagtga gcggggcggc cggatgcccg
      541 gccgccccga gtgcaaggag gattacttct tctcgagagc cagcgtcatc gtcggcgcgt
      601 cgcagacctc ggcggggccg aagtactgga tcgggccggg gaagatatag tgatcctcga
      661 gcgcccactt gtcgcggttc tcggcgaaga ccttgaacgg cttgccgtcc agatccacca
      721 acgccttctt gatcaccggg accatcttgc ccttgcgctt ctccaggttc agcatcatcg
      781 tcacgggcgt gccaccggca acccactgat ccaccggctt ggtcacgttc tggacattga
      841 cggtgtaccc ggtcaggccg ccacggatga gatgcgcggc agcatagccg agggagtagg
      901 tgtagtcggc atcgaagttc gagggcgcgg cgcagcgacc ctcatacccg aagaagtgcg
      961 agagtgggct aaacttcgct tcggtttcgc cacgcgcctt catcaggcgg atcttatccg
     1021 aaaccaaatc gatgagcagg cgctccgtct ccacctggga gaggggcacg ttgccgtggc
     1081 tatcgcgcgt gaggagaacc tcctggatgg cgaccgggag cgagttgtag acctgggcgc
     1141 tctcggcggt caactgagtg ttgaggtact gcgcgcgctc gctgtggtcc ggcaggctgc
     1201 gcagatactg ctcgtcgcga ccaaggatgg tgctcagctc gtcgatcatc accttgatat
     1261 cggggatgaa ctccaccagg ccctcaggaa cgaggagcac gccgtagttc ttgccggcgg
     1321 cggcacgctt caccacgaca tcgacgatgt agtcgacgat ctcggcgagc gtggtgccct
     1381 ttgcctgcac ctcctcggag atcagggtga tattcggatg cgtctgcagc gcgcactcca
     1441 gggtgacgtg gctggcagcg cggcccatca ggcgaatgaa gtgccagtat ttcacggcgg
     1501 aggtggcatc gcgctcgatg ttgccgatca gctccgaata ggtcttcgcc gcggtgtcga
     1561 agccaaagga ggtttcgatc tgcgcgttct tcatgtcgcc atcgatcgtc ttcggcacgc
     1621 cgattacgca ggtggggcaa tcctgcgcgc gcaggtactc ggcgaggagc gcggcgttgg
     1681 tgttggagtc gtcgccgcca acgatcacca ggccgtccag acccatctgc tcgaggttgg
     1741 ctttgcacgc ggccaggtcc tcgggcttct cgatcttgtc gcgcccgctc atgatcatat
     1801 cgaagccgcc ggtgttgcga tagcgatcga tcacatcaga ggtcagctct ttatacttgc
     1861 ccttgatgat tccgccaggg cccttcagga agccaaagag acggctctcg gggttcgcgg
     1921 ccttcagcgc atcgaacagg ccggcgatga cgttgtgtcc gcccggggcc tggccgccgg
     1981 agaggacgac gcccaccttc agcggcgccg tgctgagcga gccgtcgccc tcggcgaggg
     2041 tggcaaccgg ttggccgtag gtgtgcggaa agagcttctt gatctcctcc tggtcgctcg
     2101 ccgactccgt cggtgcccca agaacgacct tcacgttacc tgcgcgcagc gccggcggca
     2161 acttcggctg atagctggcg cgcgccttct gcaaagcaga aatctgacaa tccatcgttc
     2221 gtatccctct ttgagcccgt gtattccccg aaatgacaat atgtgaggag gggtccgggg
     2281 tgatacccac ccgtcggccg ctgcctcgaa gcccgcgggc cgtaccagat gctggccgag
     2341 gaggcatgca gaggccggat ctgtcctgga tcgcggagcg cgatgcgcag cttgccagaa
     2401 gggggctgcc ccacggcagt cctgaaccgg cggcctgccc actgcccttc gacgatccgc
     2461 ccagactcga ttcgattgcc atccagcggc ttccttcccg ctcgctggcg catcgagcct
     2521 tgcttacacc cgccgcattc ggcggggagc cactccgttt ctcccctatt atagcacaat
     2581 ctct
//
LOCUS       DAOSCV010000659         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26052_length_2582_cov_3.552038, whole genome shotgun sequence.
ACCESSION   DAOSCV010000659 DAOSCV010000000
VERSION     DAOSCV010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26052_length_2582_cov_3.552038"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2189
                     /locus_tag="PLY56_14630"
     CDS             <1..2189
                     /locus_tag="PLY56_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ22761.1"
                     /translation="MAARAVHRLHEQGVPLGRIAVVQRRLDGAATLLKSTFARYEIPV
                     AFHAGEPLGENPLVSVVSSLIRLFLNDWPRAELLRCAKSSYFGLPPEEAALLERWAIT
                     RGVRGGQRAWREYARRAGGSGAIPQWINHLGVWQGRLRNPADPFVFAKLMVLMAGDFR
                     LTERARSGDPRLAAEDERAWRSALSLLWEIAAVESIANEGPVEFSAFADAVLSGWASS
                     LFNMPEPEEEHIEILEAFDARQQEFDAVFLLALNERIFPRVVGDDPFLRDDEREVLAR
                     HGIRLARQVELADEERALFYRVITAPSRHLVLSYARTGADGSQTLPSYYLEEVRGVFA
                     EGDGAPPTIARTLADVVPDLEDAVGLREKALCAAAAQAGYRTLDAPVLDALLAELAAA
                     KPELFANWQGFLRPWNGHVLRSEPLREWVQRQARDRCFSPSELESFLACPHRHFLSAQ
                     LRIREVSEEAGAEEIGTLLHAVLRVFFRECSREAGPDAESFLPPDAMERLEAILDALL
                     PEVIHDPRRWRVRMIHHAAREYLERFLEEEYEYLLQTRLVPSYFELAFGPRSASHGDA
                     PDPASSATPLVISSPDSDVEIAGTIDRVDRTPNGEAVVVTDYKLSRGKQLKEMLAGTS
                     LQMPLYLLAMEQVFGLPAVAAVYRPLREEGPYWLFRPGLVRDLKPCGKGDGVPAARYA
                     ELIEVTRAAVLVAAGGIRAAECAPMPGEICDFCPFPDICRPDEAAV"
     gene            2201..>2582
                     /locus_tag="PLY56_14635"
     CDS             2201..>2582
                     /locus_tag="PLY56_14635"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ22762.1"
                     /translation="MPDPTPTQREAIQMLDRNLLVSAGAGSGKTTVLVNRFLRMVAGD
                     ADPTAPAEPTAPVDGVLTITFTDKAAGEMRERIAAALAARGLVEERRRLEVAHISTIH
                     GFCRRLLQENPFEAGLDPRFATIEG"
BASE COUNT          371 a          871 c          901 g          439 t
ORIGIN      
        1 agatggcggc gcgcgcggtg catcgcctcc acgaacaggg agtgccgctg gggcgcattg
       61 ccgtggtgca gcgccggctg gacggggctg ccaccctcct caagagcacc ttcgcgcgct
      121 acgagatccc cgtcgccttc cacgcgggcg agccgctcgg cgagaacccg ctggtctccg
      181 tggtctcgtc gctcatccgg ctcttcctga acgactggcc tcgtgccgag ctcctccggt
      241 gtgccaagtc gagctacttc gggctccctc ccgaggaagc ggcgctgttg gagcggtggg
      301 caatcacccg tggcgtgcgg ggagggcagc gggcatggcg cgagtacgcg cgccgtgcgg
      361 gaggctcagg cgcgatccca cagtggatca accacctggg ggtgtggcag gggcgcctgc
      421 gcaacccggc cgatccgttt gtcttcgcca agctgatggt gctgatggcg ggcgacttcc
      481 gcctgacgga acgcgcgcgc tccggggatc cccggctggc ggccgaggat gagcgcgcct
      541 ggcgaagcgc gctctccctc ctctgggaga tcgcggctgt ggagagtatc gccaacgagg
      601 ggcccgtcga gttcagtgct ttcgcggacg ccgtgctctc cggatgggcc tcgagtctct
      661 tcaacatgcc cgagcccgaa gaggagcaca tcgagatttt ggaagcgttt gacgcgcggc
      721 agcaggagtt cgacgcggtc ttcctgctcg ccctcaacga gcgcatcttt ccgcgcgttg
      781 tgggcgacga tcccttcctg cgcgacgacg aacgcgaggt gctagcgcgg catgggattc
      841 gcctggcgcg ccaggtggag ttggccgatg aggagcgcgc cctcttctac cgcgtgatca
      901 cggcgccctc gcggcatctc gtcctctcct acgcgcgcac gggtgccgat ggcagccaga
      961 cgctcccctc ctactatctg gaggaagtac gcggggtctt tgccgaaggc gatggggcgc
     1021 cgcctacgat cgcgcgcacg ctggcagacg tcgtgccgga cttggaagac gcggtgggcc
     1081 tgcgcgagaa ggcgctctgc gccgctgccg cgcaggcagg ttaccggacg ctggatgcgc
     1141 ctgtgctgga tgcgcttctc gccgagctcg cggcggcgaa gcctgagctg tttgccaact
     1201 ggcagggctt cctgcgtccg tggaacgggc acgtgcttcg ctccgagccg ctgcgcgagt
     1261 gggttcagag gcaggcccgc gaccggtgct tcagcccctc ggagctggag agtttcctcg
     1321 cctgcccgca ccggcacttt ctcagcgcgc agcttcgcat ccgcgaagtg agcgaggaag
     1381 cgggcgccga ggagatcggc acgctgctcc atgccgtcct tcgcgtcttc ttccgcgagt
     1441 gcagccgcga agccgggcct gatgccgaga gcttcctgcc gccggacgcc atggagcgcc
     1501 tggaagcgat cctggatgcc ctcttgcccg aggtgatcca cgacccgcgc cgctggcgcg
     1561 tgcggatgat ccatcatgcg gcgcgtgagt acttggagcg cttcctggaa gaggagtacg
     1621 aatacctgct tcaaacccga ttggtgcctt cgtacttcga gctcgcgttt gggcctcgga
     1681 gtgccagcca cggtgatgcc cccgaccccg cctcgtcggc gacgcccctg gtgatctcca
     1741 gccccgacag cgatgtggag atcgcgggga cgattgaccg cgtggaccgc accccgaatg
     1801 gagaggccgt ggtggtcacc gactataagc tctcccgagg caaacagctg aaggagatgc
     1861 tggccgggac gagcctgcag atgccactct acctgctggc gatggagcag gtgttcggcc
     1921 tgcctgcggt cgccgccgtt taccggcccc tgcgcgagga gggcccctac tggcttttcc
     1981 gccccggcct ggtgcgcgac ctgaaaccgt gcgggaaggg cgacggcgtg cctgcggcgc
     2041 gttacgcgga gttgattgag gtgacgcgcg ccgccgttct ggtggcggcc ggcggcatac
     2101 gcgccgccga gtgtgcgccg atgccgggcg agatctgcga tttctgcccc ttccccgata
     2161 tctgccggcc tgatgaggcg gccgtctgac agggagctcc atgccagatc cgacacccac
     2221 ccaacgcgaa gcgatccaga tgctcgaccg gaacctgctc gtctccgccg gcgcgggatc
     2281 cggcaagacc accgtcttgg tcaaccggtt tctgcgcatg gtggccggcg acgcggatcc
     2341 cacggccccg gcggagccca cggctccggt ggatggcgtg cttactatca ccttcaccga
     2401 caaggctgct ggcgagatgc gcgagcggat cgccgcggcc ctcgcggcac gcggcctcgt
     2461 ggaagagcgc cggcgcctgg aggtcgcgca catcagcacg atccacggct tctgccggcg
     2521 cctgctccag gagaatccct tcgaggcggg gctcgatccg cgcttcgcca ccattgaggg
     2581 gg
//
LOCUS       DAOSCV010000660         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26210_length_2572_cov_2.843464, whole genome shotgun sequence.
ACCESSION   DAOSCV010000660 DAOSCV010000000
VERSION     DAOSCV010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26210_length_2572_cov_2.843464"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="PLY56_14640"
     CDS             <1..831
                     /locus_tag="PLY56_14640"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOJ22763.1"
                     /translation="GTMFLMWMGERITDKGIGNGVSLVIFVGIMVQLPYDLGRTIQVV
                     GTQGNYTGAVALMVIFLATVAAIVAITQGQRKIPIQHAKRVVGNRMYGGGSSFLPLKV
                     NQAGVIPIIFAVSVVSLPAQIAQFAPVGEGWLRTFFDKLIPWFSPGAPGLPGFFATSL
                     YALLVLFFTYFYTAVTFDVPKVSDDLRKYGSFIPGIRPGRPTADYLDKVMTRITLAGA
                     VFLAVIALMQYYVPAWTGVTTFSLVGGTSLLIVVGVALDTMQNIEAHLIMRHYEGFIK
                     "
     gene            924..1709
                     /gene="map"
                     /locus_tag="PLY56_14645"
     CDS             924..1709
                     /gene="map"
                     /locus_tag="PLY56_14645"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ22764.1"
                     /translation="MKRAKRRVYLKSEEELQTMRRANQLVASTLRLLRDAVQPGITTA
                     ELDRIAEENIRDHGGVPSFKGYHGFPASACISVNDQVVHGIPGPRRLREGDIVSIDLG
                     AIVDGFHGDSAITVPVGTISAEARALLYHTYRALWEGIAQAVVGNRVGDISHAIQRYA
                     EAQGYSVVRELVGHGIGRNMHEEPQVPNFGPPGRGPALMPGMTLAIEPMVNLGRPEVE
                     VAPDQWTVSTQDGKVSAHFEHTIAVTLDGPMVLSEWPDQEEPI"
     gene            1827..2045
                     /gene="infA"
                     /locus_tag="PLY56_14650"
     CDS             1827..2045
                     /gene="infA"
                     /locus_tag="PLY56_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ22765.1"
                     /translation="MPKENAIEVEGTVLETLPNAMFRVELQSGHRVLAHVSGKIRMHF
                     IRILPGDRVLVELSPYDLSRGRIIYRYK"
     gene            2260..2373
                     /gene="rpmJ"
                     /locus_tag="PLY56_14655"
     CDS             2260..2373
                     /gene="rpmJ"
                     /locus_tag="PLY56_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOJ22766.1"
                     /translation="MKVRPSVRKLCDKCQIIRREGVVRVICNKPKHKQRQG"
BASE COUNT          450 a          748 c          902 g          472 t
ORIGIN      
        1 gggacgatgt tcctgatgtg gatgggcgag aggattaccg acaaggggat cggcaacggg
       61 gtctcgctgg tcatcttcgt gggtatcatg gtccagctcc cctacgacct tgggcgtacc
      121 atccaggtgg tgggcacgca ggggaattac accggtgctg tggccctcat ggtcatcttc
      181 ctcgcgacgg tggccgccat cgtggcgatt acgcagggcc agcgcaagat accgatccag
      241 cacgccaagc gcgtcgttgg aaaccgaatg tatgggggcg ggagcagctt cctgccgctg
      301 aaggtaaacc aggccggcgt catcccgatc atcttcgccg tctcggtcgt ctctctgccg
      361 gcgcagattg cccagttcgc cccggtcggc gagggctggc tgcgcacgtt ctttgataag
      421 ctgatcccct ggttctcgcc aggggctccg ggcctgcccg gcttcttcgc cacatcgctc
      481 tacgcgctgc tggtgttgtt cttcacttac ttctataccg cagtgacgtt cgatgtgccg
      541 aaggtctcgg atgacctgcg caagtacggc agcttcatcc ccggcatccg cccgggacgg
      601 ccgacggcgg actacctgga caaggtgatg acgcggatca ccctggcggg cgcggtcttc
      661 ctggccgtga tcgccctgat gcaatactac gtgccagcct ggactggggt gacgaccttc
      721 agtctcgtcg gtggcacgtc gctcctcatc gtggtgggtg tggcgctcga tacgatgcag
      781 aacatcgagg cgcacctgat catgcgccac tatgaggggt tcatcaagta ggaatcgcgg
      841 ggggcccggg cggtgatgac cgcccggcgc cctatcgcgc gtgagccggt tgtagtggct
      901 cgcagaggag gagagaacag ctcttgaaac gggcaaagcg tcgggtctac ctgaagagcg
      961 aagaggagct ccagacgatg cgccgcgcga atcagttggt tgcctcgacg ctgcgcttgt
     1021 tgcgggacgc cgtccagccc ggtatcacca ccgccgagct ggaccggatc gccgaggaga
     1081 acatccgaga ccatggcggt gtgccctcgt tcaagggcta tcacggtttc cccgcgagtg
     1141 cgtgcatctc ggtgaatgac caggtggtgc atggcattcc cggaccgcgc cggctccgag
     1201 agggcgatat cgtaagtatc gacctagggg cgattgtgga tggcttccac ggcgactccg
     1261 cgatcacggt gccggtgggg acgatcagcg cggaggcgcg cgctctcctg tatcacacgt
     1321 atcgtgccct gtgggagggg attgcgcagg cggtagttgg aaaccgcgtg ggcgacatct
     1381 cccatgccat tcagcgctac gcggaggcgc aaggctattc cgtggtgcgg gagctcgttg
     1441 gccacggaat cggccggaat atgcacgagg agccccaggt gcccaacttt gggcctccgg
     1501 gccgcgggcc ggcgctgatg ccgggcatga cgctggccat cgagccgatg gtcaaccttg
     1561 ggcgccccga ggtggaggtc gcccccgacc aatggacggt gtcgacacag gacggaaagg
     1621 tgtctgcgca tttcgagcac accatcgccg tcaccctgga tggcccgatg gtcctctctg
     1681 aatggccgga ccaggaggaa ccgatctgag gcggccagta gcgggcgggg ccgtcggtga
     1741 ggatgcccgg ccgaccagcg cgacggcagg cgtgagccgg gcgaacgcga gcgcccccgt
     1801 ctgagcgagg agtgagacga aacagtatgc ctaaagagaa cgccatcgaa gtcgaaggta
     1861 ccgtgctgga gacgctgcct aacgccatgt tccgggtgga gctgcagagt ggccaccggg
     1921 tgcttgccca cgtctcgggc aagatccgga tgcacttcat ccgcattctg ccaggcgacc
     1981 gggtgttggt ggagctctcg ccgtacgacc tatctcgggg ccggatcatc taccggtaca
     2041 agtaggaccg tccggctcac gcgcggtagg gcgaggctgc gggcactttc ggtttggcat
     2101 cctgcgggcg gggagcgctc gtgcgcagga tctggtcaga aatctcggat tgtgctacaa
     2161 tgtagccttg cggggcgttt tgggcttgcg acgaatcgct tttgggccgg gccgacggag
     2221 ttgccgtggt ccgggcattg ctgaggaagg aataccgtga tgaaggtacg accatctgtc
     2281 cggaagttgt gcgataaatg ccagattatt cggcgcgaag gcgtggtccg tgtgatctgc
     2341 aacaagccga agcataagca gcgccaggga taaggcgcgt gcgcgggtgt gtccggagcc
     2401 tctgcatcgc gatctctcgt gtaggccggc tcgccagggg tacccgcgaa gtggacgcgt
     2461 gcggaacggg catccgccgg ggatgggaga gccacccggc gggccgggcc ggaggaagag
     2521 acgttacagc ggcatctgag gcacagatgc actgggaggt aacacgttgg cg
//
LOCUS       DAOSCV010000661         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26244_length_2570_cov_3.419085, whole genome shotgun sequence.
ACCESSION   DAOSCV010000661 DAOSCV010000000
VERSION     DAOSCV010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26244_length_2570_cov_3.419085"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..2497)
                     /locus_tag="PLY56_14660"
     CDS             complement(440..2497)
                     /locus_tag="PLY56_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOJ22767.1"
                     /translation="MERQRRQARPAGQRGAAMTPAVESAFIHLEPISPEDALEREWLC
                     TNGLGGYAMGSIAGACTRRYHGLLVAALKPPLGRTVLCAKVDETFETSGETLALGANE
                     FADGTRAPWGVTHLSSFRLEGARPTWRYAVPGGILQKSIWMVYGRSTTCLRYLTEQTG
                     GRLRLAVFATYRDYHHETRGSTEWHFDIRPVAAGAEVVAFPGATPWRILGPTGACFTP
                     IGDWWWRFHHRHERARGLDCEEDLLLVGTLEFPLSPGRAAFVVITSEPATPEGSTPEL
                     CPAREWKRQLARESELVRRAGAEADPVRAQLVLAADQFLVRRGNAPRGEAGTILAGYP
                     WFSDWGRDTMIALPGLTLSTGRYSEARRILVTYARYLDQGMLPNRFPDGGEAPDYNTV
                     DATLWFFQAVAAYTAASGDETLVAELFPALDEVVAWHLCGTRYGIGVDPGDGLLRAGE
                     KGSQLTWMDAKVGDWVVTPRQGKPVEINALWYNALCHMHAWAERLGLPPRPRASYSAS
                     YAELAARARESFNARFWYPQGYLYDVLDTPEGPADSRLRPNQLFAAALPHPILERGRA
                     QTMLATVRRHLLTPVGVRTLAPDDPDYQGWYAGDQWHRDRAYHQGTVWPWLLGGYVDA
                     RRRLGASAAELRGLTRELVQHLGQAGVGSISEVFDGTPPHHPGGCPAQAWSVAELLRI
                     LSE"
BASE COUNT          388 a          837 c          949 g          396 t
ORIGIN      
        1 cgttggggtt ccaggtgtct tcttgacacg acacccaggg tcaggagacc ctgggctggg
       61 ctatgggacc gcttcgcgtt cctccgaggg cgtctcgcat ggcccatgcc cggatcccgg
      121 atggattgca cagcccctgg tgcctgcgcc tctccgtccg tgtccgtccc cttcatccgt
      181 ggtgtcaccg gcggggcgca acccggcgcg cggggatcca tccccgcgct gcttgtgagc
      241 gaagccctgc cgggctgggt tgggcgacac ccccggatgc ctcaaaatgg cgagggctgg
      301 gagggggagc ccgaagggct ttgctgggaa gcagcgcgga gcttccagct ccgcgcgtgt
      361 gggcggccca caccatccgt gtccgttccc ttcatccgtg gtaacgacgc ccggcgggtg
      421 gatggtcaca aaggcatatt cactcgctca ggatgcgcaa cagctcagcg acgctccagg
      481 cctgggccgg acagccgccg gggtggtgtg ggggcgtgcc atcgaacacc tcgctgatgc
      541 taccgacgcc ggcctgcccc aggtgttgga ccaactcgcg ggtgaggcca cgcagctccg
      601 ccgcggaggc gccgaggcga cgccgagcgt ccacgtagcc accgaggagc cagggccaga
      661 cagtaccctg atggtatgcc cgatcgcggt gccattgatc gccggcatac cagccctggt
      721 agtcggggtc gtcgggcgcg agcgtgcgca cgccgaccgg cgtgagcagg tgccggcgga
      781 cagtcgcgag catcgtttgg gcgcgcccgc gctccaggat ggggtggggg agcgcggcgg
      841 cgaagagctg attcgggcgg aggcgggagt cggcggggcc ttccggcgtg tcgagcacgt
      901 cgtagaggta gccctgcggg taccagaagc gcgcgttgaa gctctcgcga gcgcgagcgg
      961 ccagctcggc gtaggaggcg ctataggatg cgcgcgggcg aggcgggagc cccaggcgct
     1021 ctgcccaggc gtgcatgtgg cagagcgcgt tataccacag tgcgttgatt tccaccggct
     1081 tgccctggcg gggggtgacc acccagtcgc cgaccttggc atccatccag gtgagctgcg
     1141 agcccttctc cccggcgcga agcaggccat ctcccgggtc gacgccgatc ccatagcgcg
     1201 tgccgcagag gtgccacgct accacttcat caagcgccgg gaagagctcg gcgacgagcg
     1261 tctcgtcgcc gctggccgcc gtgtaggcgg cgaccgcctg gaagaaccag agtgtggcat
     1321 cgacggtgtt gtagtcgggg gcttcacccc catcggggaa gcggttggga agcattccct
     1381 ggtccaggta tcgggcgtag gtgaccagga tgcgccgcgc ctcggagtag cgtccggtgg
     1441 agagggtgag cccgggcagg gcgatcatcg tgtcgcggcc ccagtcgctg aaccaggggt
     1501 agccggccag gatggtgccg gcttcgcccc ggggtgcatt gccacggcgc accaggaact
     1561 ggtcggcggc gaggacgagt tgggcgcgca cgggatcggc ttcggcgccc gcgcggcgga
     1621 cgagctcgct ctcgcgcgcc agctgccgct tccactcgcg ggcggggcag agctccgggg
     1681 tgcttccctc gggagtagcg ggttcggagg tgatgacgac gaaagcggcc cggccgggcg
     1741 atagcgggaa ctccagggtt ccgaccagga ggaggtcctc ctcgcaatcg aggccacgcg
     1801 cgcgctcgtg gcggtggtgg aagcgccacc accaatcgcc gatgggcgtg aagcacgccc
     1861 cggtggggcc gaggatgcgc cagggagtag ccccgggaaa tgccaccact tcggcacctg
     1921 cggcgacggg acggatatcg aagtgccact ccgtggagcc ccgcgtctcg tggtggtagt
     1981 cgcgatacgt ggcaaaaacg gccagacgga ggcgcccgcc ggtctgctcc gtgagatagc
     2041 gcaggcaggt cgtcgagcgg ccgtagacca tccagatgct cttctggagg attcccccgg
     2101 gaacggcata gcgccaggtg gggcgcgccc cctccaggcg gaacgatgag aggtgggtga
     2161 caccccaggg ggcacgcgtg ccatcggcaa actcgttggc gcccagggcg agcgtctcgc
     2221 cggacgtctc aaacgtctcg tccaccttgg cgcacaggac ggtgcgcccc agggggggct
     2281 tgagtgcggc aacgagaagg ccgtggtagc gcctggtgca tgcacccgct atgctgccca
     2341 tcgcgtagcc gccgaggccg ttcgtacaca gccactcgcg ctcgagcgca tcctcgggag
     2401 agatcggctc gagatggatg aaggcggatt ccacggcggg cgtcattgcc gcgccgcgtt
     2461 gccctgcagg ccgcgcctgc ctccgctgtc gttccatgag cttccctctg ttcgggtgtt
     2521 ctcccacctg ggggcagcgc gatggccgcc cctaggccct cattattcac
//
LOCUS       DAOSCV010000662         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26262_length_2569_cov_2.643596, whole genome shotgun sequence.
ACCESSION   DAOSCV010000662 DAOSCV010000000
VERSION     DAOSCV010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26262_length_2569_cov_2.643596"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLY56_14665"
     CDS             <1..704
                     /locus_tag="PLY56_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOJ22768.1"
                     /translation="TRVIAISQATADDIAELYGVPRERIVVTPLAPDPRFQPVPDAAA
                     RVAARYGLRSPYLLFVGTPYPRKNLARVLDAFAQLGPAGKGVTLVIASRKEEAAAEVL
                     ARARVLGVEEQVRFLAGVPDEEMPALFSAAQMLVHPALCEGYGLTLVEAMACATPVIT
                     ANRSSMREVAGDAALLVDPEKPGEIAGAIRRILTDPEARETCIRRGAERVAGLTWERT
                     ARLTLDAYRAAHEGK"
     gene            713..1666
                     /locus_tag="PLY56_14670"
     CDS             713..1666
                     /locus_tag="PLY56_14670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22769.1"
                     /translation="MVSVVATVWNEAGSLQEFLRALLAQTLPADEIVISDGGSTDGTR
                     ELLQEVAHEEPRVHPILLPRSNRSVGRNAAIEAARNEIIAVTDAGSRADPDWLERITA
                     PLRADPTVCMVGGYYRALARSPLQEAIAAATVVPPERVDPETFLPSSRSLAFRKEAWA
                     RVGGYPEWSDHNEDTIFALALRRAGCRMVFVPDAVVSWEPSPDLRRLFKQFYRYARGD
                     AQAGLFFGHYWKNLAIPAAGAAYLLGRRHPRLRAAVGTGVGLYLAKQSLRALRQTRSA
                     AATALTPAVALTLDMAHLTGYVHGILDRPAIRRAREDAPDE"
     gene            1787..>2569
                     /locus_tag="PLY56_14675"
     CDS             1787..>2569
                     /locus_tag="PLY56_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ22770.1"
                     /translation="MDLLEQLNPVQREAVTHPDGPLLIFAGAGSGKTRVLTHRVAWLI
                     RERGVHPAHILAVTFTNKAAKEMKTRIASLVGSLEESIWIGTFHALAARMLRMHGEHI
                     GLDPGFVIYDDKDQETVVKECYRELDIDEKKYAIRATLNAISRAKERLIEPREYGRTA
                     MDSFERVVANVYPAYNHKLRLNNALDFDDLIGCAVKLLREAPSVREEIQHRFRQVLVD
                     EFQDINHAQYAWMRLVAGKHGNVTVVGDDDQSIYSWRGADVGF"
BASE COUNT          410 a          844 c          881 g          434 t
ORIGIN      
        1 cgacgcgagt aatcgcgatc tcgcaggcga ccgccgatga tatcgcggag ctttacggcg
       61 tgccgcgcga gcgcatcgtt gttacgccgc tcgcgccgga tcctcgtttt cagccggtgc
      121 cggacgcggc ggcgcgtgtc gccgcgcgct atggcctgcg ctcgccgtat ctcctcttcg
      181 tgggcactcc ctacccgcgg aagaacctcg cgcgggtgct cgatgccttc gcccagcttg
      241 gtccggcagg gaagggggtc accctcgtga ttgcgtcgcg caaggaagag gcggccgcgg
      301 aggtgctggc acgggcgcgg gtcctcggcg tggaggagca ggtgcgcttc ctggccggcg
      361 tgccggatga ggagatgccg gcgctcttct cagcggccca gatgctggtg catccggcgc
      421 tgtgcgaggg ctacgggctc acactggtgg aggcgatggc gtgcgctact ccggtgatca
      481 ccgcgaaccg gtcgtcgatg cgcgaagtgg cgggagatgc ggcgctcctg gtcgatccgg
      541 agaaaccggg cgagatcgcc ggcgccatcc ggcgcatcct gaccgatccg gaggcacgcg
      601 agacgtgcat ccgccggggc gctgagcggg tggccgggct tacctgggag agaacggcgc
      661 gcctgaccct ggatgcttac cgcgcggcgc acgaagggaa gtgagggacg cgatggtctc
      721 cgttgtagct accgtgtgga atgaggccgg gtcgttgcag gagttcctcc gggctctcct
      781 cgcgcagacg ctccccgcgg atgagatcgt gatctctgac ggcgggtcca ccgatggaac
      841 gcgcgagctt ctccaggagg tggcgcacga ggagccgcgc gtgcacccga tcctgttgcc
      901 gcgatccaac cgctctgtgg gtcgcaacgc cgccatcgag gctgccagga acgagatcat
      961 cgccgtgacg gacgccggct cgcgcgccga tcccgactgg ttggagcgga tcaccgcgcc
     1021 cctgcgcgcc gacccgacag tgtgcatggt gggcggctac taccgggcgc tcgccaggag
     1081 cccgctccag gaagcgatcg ccgcagccac ggtggtgcca ccggaacgcg tggatcccga
     1141 gacgttcctt ccaagcagcc gatcgctggc ctttcgcaag gaggcgtggg cgcgggtagg
     1201 cggctaccca gagtggtcgg accacaacga agatacgatc tttgccctgg cgttgcgccg
     1261 ggccggctgt cgcatggtgt tcgttccgga cgcggtcgtc tcgtgggagc cttctcccga
     1321 cctgcgccgg cttttcaagc agttctaccg ctatgcgcgc ggcgatgcgc aggcggggct
     1381 cttcttcggg cactactgga aaaacctcgc catcccggcc gcgggagcgg cctacctcct
     1441 cgggcgccgg catccgcggc tgcgcgccgc ggtgggcacc ggcgtgggcc tttatctcgc
     1501 gaagcaatcc ctgcgcgcgc tgcgccagac acgtagcgcc gcggccaccg cgctcacccc
     1561 cgccgttgcg ctgacgctgg atatggccca cctgacggga tacgtccacg gcattctcga
     1621 ccggccggcc attcgccggg cgcgcgagga tgcgcccgac gagtgaggaa agaccgcctc
     1681 ttccttgact tcgcaccggg gacgtggtac actgccaggt ggcgccacgc ctcgcgcggt
     1741 gcgggcgcca gttgccatct actaccgcgc gtggatgcct gtacttatgg atctcctgga
     1801 acagttgaac ccggtgcagc gtgaagcagt gacgcatccg gatggaccgt tgctcatctt
     1861 tgccggcgca ggctcgggga agacgcgcgt gctcacccac cgagttgcct ggctcattcg
     1921 cgagcgcggg gtgcaccccg cgcacatcct cgcggtcacg ttcacaaaca aggccgcgaa
     1981 ggagatgaaa acccggattg ctagtcttgt cggctccctg gaggagagca tctggatcgg
     2041 caccttccac gctctcgccg cgcgcatgct gcggatgcat ggagagcata tcggcctgga
     2101 tcctggcttc gtgatctacg acgacaagga ccaggagacc gtggtgaagg agtgctaccg
     2161 ggagcttgat atcgatgaga agaaatacgc tatccgggcc actctcaacg cgatcagccg
     2221 ggccaaggag cggctgatcg aaccccggga gtacggccgc actgcgatgg actccttcga
     2281 gcgcgtcgtt gccaacgtct acccggccta caatcacaaa ctgcgcctca acaacgcgct
     2341 cgatttcgac gacctcatcg gctgcgccgt aaagctgctc cgcgaggccc cctcggtgcg
     2401 cgaggagatc cagcatcgct tccgacaggt gcttgtggac gagtttcagg atatcaacca
     2461 cgcgcagtat gcctggatgc gcctggtggc cgggaagcac ggcaacgtca ccgtggtcgg
     2521 cgacgatgac caatcgatct actcgtggcg cggcgccgat gtcggcttc
//
LOCUS       DAOSCV010000663         2566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26293_length_2566_cov_3.954998, whole genome shotgun sequence.
ACCESSION   DAOSCV010000663 DAOSCV010000000
VERSION     DAOSCV010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26293_length_2566_cov_3.954998"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="PLY56_14680"
     CDS             <1..1842
                     /locus_tag="PLY56_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HOJ22771.1"
                     /translation="IEPLEGGRSAIVITEIPFMVNAQRLIEEQSAGLVKEKKLPEVSA
                     IHDHSDRHGMRIVVELKRDSQPRKVLNYLLKHTSLRCNFNVMMLALVDRQPRVLSLRE
                     MLHYYVEHRKDVITRRSNHELRQLKDHAHILEGLRIALFNLDPLIQTIKESPDPATAR
                     VRLVERFGLSHPQAVAILQMQLQRLTGLEQRKIEDDYRQTVKRIAYLEDLLSDPEKIL
                     GVIRQDLKEIRDKYGDDRRTRIIAREASDIGEEDVIAEEDTIVTITRDGYIKRVPIDT
                     FRSQHRGGRGVIAANAKEEDSLSQLFIATTHHYVLFFTDKGKVYRLKAYEVPQASRTA
                     MGTAIINLIPIESGERITATVPVRSLEADGYLVMATERGVVKKTRIAEFNTARKGGLI
                     AMNIEPGDELRWVDVTDGECEIMLITREGHAVRFHEQQVRPMGRAAAGVRGVSLRPGD
                     QVVAMTVSRPEADLLVVTDKGYGKRTPVSEYRTSNRGTMGVITMKLGEKVGHIVDAKV
                     VDDDDKLLMMTENGIAIRIRVSDIRRCGRSTQGVRLINVNENDHVARVERVVRKQHHA
                     SEAETDGTLADQAVGNGKVKASQPRAAHAEMDAEIDAETNGKADTEE"
     gene            2095..>2566
                     /locus_tag="PLY56_14685"
     CDS             2095..>2566
                     /locus_tag="PLY56_14685"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ22772.1"
                     /translation="MAHRYTSTRQFFLDLIRDYEPELTFRGTTREEFDSWQAAFRQRF
                     LECLGPLPPAVPFETEVLWRIEEDGLIKEKILFDTAPFTTVPAILLRPADTEQGRRRT
                     AIVCIHGHGPHGKDPVAGARYPEAQQNASYYNYNYGEQMARQGFVTICPDMRPFG"
BASE COUNT          523 a          852 c          792 g          399 t
ORIGIN      
        1 atcgagccgc ttgaaggcgg gcgctcggcg atcgtcatca ccgagatccc cttcatggtc
       61 aatgcccagc gcctgatcga ggagcagagt gccggcctgg tcaaagagaa gaagctcccc
      121 gaggtgagcg ccattcacga tcactccgac cggcacggca tgcgcatcgt ggtggagctg
      181 aagcgcgact cgcagccgcg gaaggtgctc aactacctcc tgaagcacac atccctgcgc
      241 tgcaacttca acgtgatgat gctggcgttg gtcgaccgcc agccgcgcgt tctctcgctc
      301 agggagatgc tccattacta cgtggagcac cgcaaagatg taatcacgcg ccggagtaac
      361 cacgaactgc gccagctcaa ggatcacgcg cacatcctgg agggcttgcg cattgcgctc
      421 ttcaacctcg acccgctgat ccagacgatc aaggaatcgc cagatccggc cacggcgcgc
      481 gttcgcctgg tggaacgctt cggcctctcc cacccgcagg ccgtcgccat ccttcagatg
      541 cagctccagc gcctcaccgg cctcgagcaa aggaagattg aggatgacta tcgccagacg
      601 gtgaagcgca tcgcctacct ggaagacctc ctctccgacc cggagaagat cctcggggtg
      661 atccggcagg atctgaagga gattcgagac aagtacggcg acgaccggcg tacgcgcatc
      721 atcgcgcgcg aggccagcga cattggcgaa gaggatgtga tcgcggagga agatacgatc
      781 gtaacgatca cgcgcgatgg ctacatcaag cgcgtgccga tcgacacctt ccgcagccag
      841 caccgtggcg ggcgcggagt gatcgcggcc aacgccaagg aagaggatag cctctcgcag
      901 ctcttcatcg cgacaacgca ccactacgtt ctcttcttca ccgataaggg gaaggtgtat
      961 cgcctcaagg cctacgaggt gccccaggcg tcgcgcacag cgatgggcac ggcgatcatc
     1021 aacctgatcc caatcgagag cggagagcgg atcaccgcca ccgtgccggt gcgctcgctg
     1081 gaggccgacg gctacctggt catggccacc gagcggggag tcgtcaagaa gacgcgcatc
     1141 gccgagttca acaccgcgcg caaaggtggc ctgatcgcca tgaatatcga gccgggtgac
     1201 gaactgcgct gggtggatgt caccgacggc gagtgcgaga tcatgctgat cacccgcgag
     1261 gggcacgcgg tgcgcttcca cgagcaacag gtgcgcccga tgggccgcgc cgcggccggc
     1321 gtgcgcggcg tcagcctgcg gcctggcgat caggttgtgg cgatgaccgt ctcgcggcca
     1381 gaggccgacc tcctggtcgt cacagacaag ggctatggca agcgaacgcc ggtctccgag
     1441 tatcgcacca gcaatcgcgg cacgatgggc gtgatcacga tgaagctggg cgaaaaagtg
     1501 gggcacatcg tcgacgccaa ggtggtggat gacgacgaca agctcctgat gatgacggag
     1561 aacgggattg ccattcgcat ccgcgtctcg gatatccgcc gttgcgggcg cagcactcag
     1621 ggggtgcgcc tgatcaacgt caacgagaat gatcacgtcg cccgcgtcga gcgcgtcgtt
     1681 cgcaagcagc accacgcctc agaggccgaa accgatggaa ccctcgcgga tcaggcagtg
     1741 ggcaacggga aggtcaaggc cagccaacct cgcgcggcac acgccgagat ggatgccgag
     1801 atcgacgccg aaacgaacgg caaggctgat accgaggagt aaccccctct tccccgccgg
     1861 gccggccatc agcggtccgg cggggtttct tcttcccctg cacccccacc cgatgcatcc
     1921 cctccgaatg gtacggcacg ccctcgggaa tctcccgagg gaccacgctt ccggcgccag
     1981 tcactgcgcg agacctgacc cgcgtgcccg gcaggaagag cctggtcgat tcgagaaatc
     2041 ggtctgggtg ccggcggcga actgaacccc tgccggcgac acctggagga atcaatggca
     2101 caccgataca cctcgacccg gcagttcttc ctggacctga tccgcgacta cgagccggaa
     2161 ctcaccttcc gcggcacgac gcgcgaggag ttcgattcgt ggcaggccgc tttccggcag
     2221 cgcttccttg aatgcctcgg ccccctacct cccgccgtgc ctttcgagac agaggttctc
     2281 tggcgcattg aagaagacgg cctcattaag gagaagatcc tgtttgacac cgcgccattc
     2341 acaaccgtgc ccgccattct tctccgcccg gcggatacgg aacagggccg gcgccgcacc
     2401 gccatcgttt gcatccacgg gcacggcccc cacggcaagg atcccgtcgc cggcgcgcgt
     2461 taccccgagg ctcagcagaa cgcttcctac tacaactata actacggcga gcagatggcg
     2521 cgccagggct tcgtcacgat ctgcccggac atgcgcccct tcggcg
//
LOCUS       DAOSCV010000664         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26364_length_2561_cov_3.629290, whole genome shotgun sequence.
ACCESSION   DAOSCV010000664 DAOSCV010000000
VERSION     DAOSCV010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26364_length_2561_cov_3.629290"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            621..>2561
                     /locus_tag="PLY56_14690"
     CDS             621..>2561
                     /locus_tag="PLY56_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22773.1"
                     /translation="MLGLLLLLSHRAEAHAGARIAILDGQVRHGWTTESTRYLGVRLE
                     RDLLVVRPTDRTRAYATAALVAGPDAAPIDGRECAYLTFEINTLGGSTGAHRAPSLRV
                     GLSDSTRRTRQPLLSRYIHLDADPTTWQQVLIPLSHLVDRGVRADALTCVSWQYAGST
                     APFAVRRVALAGSPGPSDLPGDGPGNLLADSSFEARADARPIWALTGDYRDEAAPGER
                     ELRRGYAAHGRRFLRLRGSRAYFFEALAPRADAVGFSVALRADRRRAEEGAQRVSLLA
                     PPDAGAPPSPGPVAAQDARAGAGRAEIGCEVVGFGGGGGVAIVGRAVRTVTPEASWRR
                     HSLSAIVQPADVPPDEGTLFLYRGWVRPLDDGLDLDAVQLAPMPLRDDQYQERRANAL
                     AAGEGIFRQVRGLIAYREHSDERDEPVPPRLQSDLPPLSVPVHAREWAGRAWSPAIIS
                     GSVPLARGLVFSPDALRLRAPDGSEVPFQFDVLRRDRRDGSVRSLWLTFQAPLAAGER
                     ASYTLESGATTAPPPLLPVASREGDEVFLHTGDLLARASGKEFSLLTEPRWGDSLAPG
                     ERGGIRLELLDGTRLTSHGPPSQVVVERNGPLHAVVVVRGYLAPADGDVQQRFRYEAR
                     LRAWRGIPGMRVDLAVTNLTPEP"
BASE COUNT          360 a          870 c          884 g          447 t
ORIGIN      
        1 cgcgctgctt gcgagcgaag ccctgccggg ctgggttggg gatactccca gatgcctcaa
       61 aacagcggtg gctgggagga gaagccccga agggctttgc cggaaagcag ggtggagctt
      121 cgagctccgc gcgtgcgcgc ggtcttctcc ttatgtcgga atcccagctc acccatgcgg
      181 cacatgacaa gctcctccct ttctggtata atggcaaaga cagcaatgcc aaccaggggg
      241 ccccacgatg gcacaaggac gttacaccgg acgctcttcc ctcactctct tgtggcagca
      301 ggtggtgggg agctcttgca taccagcgtg ccgagtgaag attgacactg atgttcgatt
      361 tgcctccgct ctggagacca ggaaaatcct ggaggtcgag cgtgctgagg ccttggagca
      421 gagcgatcgc ctggatcgat tccatcaggc gctctgctgg gagcgagggt tccactagcc
      481 cagcgtcccc gcgtttcatg gccgcgtgcg cgcgtcgggg agggagggct caccaggttc
      541 caatcaccgg gagagtcttc tctacgcgcc gccatgcctc gcgcgcgtgt atcgcaggcg
      601 ctcgatgcct tgccattgga gtgcttggcc tcctcctgct gctttcgcac cgggccgagg
      661 cgcatgccgg cgcgcgcatt gccatcctgg atggtcaagt ccgccacgga tggaccacgg
      721 agagcacgcg ctacctcgga gttcgcctgg agagagatct cctcgtggtg cgtcccaccg
      781 accgcacgcg cgcctatgcc acggcggcgc tggtggccgg cccggacgcc gcgcccatcg
      841 atggccggga gtgcgcctac ctcactttcg aaatcaacac cctcggtggg tccaccggcg
      901 cgcatcgggc gccctccttg cgcgtgggtc tctccgattc cacgaggcgc acgcgccagc
      961 cactcctatc gcgatacatc cacctggatg ccgacccgac cacctggcag caagtgctca
     1021 tccctttgag ccacctcgtc gaccgcggcg tgcgtgccga cgcgctgacg tgcgtcagct
     1081 ggcagtacgc cggttccacc gcgcccttcg ccgttcgccg ggtcgcgctc gcaggcagcc
     1141 ccggtccgtc cgatcttcct ggcgatggcc cggggaatct gctggcagac tccagcttcg
     1201 aggcacgggc ggatgctcgg cccatctggg cgctgaccgg cgactatcgg gatgaggccg
     1261 cgccaggaga acgcgaactg cgccgcggct acgcggccca tggccggcgc ttcctgcgcc
     1321 tgcggggaag ccgggcctac ttcttcgagg cgctggcgcc acgcgccgat gctgtcgggt
     1381 tttccgttgc cttgcgcgcc gaccggcgcc gggccgagga gggggcacag cgggtgtcgc
     1441 tactcgcgcc tcccgacgca ggggctccgc catcgcccgg gccagtagct gcgcaagacg
     1501 cgcgagccgg cgcggggcgg gcggagatcg ggtgcgaggt ggttggcttc ggtggcggag
     1561 ggggcgtcgc catcgtcggg cgcgccgttc gcacggtgac gccggaagcg agctggaggc
     1621 gccattcgct ttcggcgatc gtccagcccg cggacgtgcc ccccgatgaa ggcactctct
     1681 tcctgtatcg cgggtgggtt cggccgctag acgacgggct ggatttggat gccgtgcagc
     1741 tggccccaat gcccctccgg gatgaccaat atcaggagcg gcgcgcgaac gcgctggcgg
     1801 caggcgaggg gatcttccgg caggtgcgcg ggctgatcgc gtatcgcgag catagtgacg
     1861 agcgcgacga accggtgccc ccccgtttgc agtccgatct ccccccgttg agcgttccgg
     1921 tacatgcccg tgaatgggcc gggcgcgcat ggtcgcccgc catcatctcc ggaagcgtgc
     1981 cgctggcccg gggccttgtc ttctctcccg acgccctccg gctgcgtgcc cccgatggca
     2041 gcgaggtccc gtttcagttc gatgtgctgc gccgtgaccg gcgcgacgga tcggtgcgca
     2101 gtctctggct gaccttccag gcgcccctgg ctgcgggaga gcgggcgagc tatacgctgg
     2161 agtcgggcgc caccacggca ccgccgccgt tgcttcctgt tgcctcccgg gagggcgatg
     2221 aggtcttcct ccataccggg gatctcctgg cgcgggcctc gggcaaggag ttctcgcttc
     2281 tcacggaacc acggtgggga gattccctcg cgccagggga gcgaggcggg atccgcctgg
     2341 agctgttgga tggaacgcgc ctcacctctc acgggccgcc ctcgcaggtt gtcgtcgagc
     2401 gaaatgggcc gctgcatgcg gtcgtggtcg tgcgcggcta cctagcgccc gcggatggag
     2461 acgtgcagca gcgtttccgc tacgaagctc ggctgcgcgc ctggcggggc attcccggca
     2521 tgcgggtcga tctcgccgtc acgaacctca ctcctgagcc g
//
LOCUS       DAOSCV010000665         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26368_length_2561_cov_3.246209, whole genome shotgun sequence.
ACCESSION   DAOSCV010000665 DAOSCV010000000
VERSION     DAOSCV010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26368_length_2561_cov_3.246209"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            470..712
                     /locus_tag="PLY56_14695"
     CDS             470..712
                     /locus_tag="PLY56_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22774.1"
                     /translation="MNAEAGVLQSPDELAPGGPLVATGAAGLPSWRVFAAATLALAAA
                     AVWFTVACQEGRFARNAMGPDSDLLSAAELEDQLNI"
     gene            887..963
                     /locus_tag="PLY56_14700"
     tRNA            887..963
                     /locus_tag="PLY56_14700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Val,seq:tac)
     gene            1273..1974
                     /locus_tag="PLY56_14705"
     CDS             1273..1974
                     /locus_tag="PLY56_14705"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOJ22775.1"
                     /translation="MMVERQTLYRWVRNGTVSRLRVALNAGLDPNHRNARRRTLLHVA
                     AASGQAEVARLLLEYGAVVDDQPDPGQTPLLEAIVKGHAEVMRVLIDYGADIWVESRE
                     GMTMLHAAAFWGDEECIQVLVDAGLPLDMADDAGMTPLHYAARFSPAYRAETVRYLVQ
                     LGARVNVRDRRGQTPLHLAASTSDPVVVMALLACGANPRIRDDEGRTAWDVADGRDGE
                     VIRALLEVALANDRE"
     gene            2315..>2561
                     /locus_tag="PLY56_14710"
     CDS             2315..>2561
                     /locus_tag="PLY56_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22776.1"
                     /translation="MEAIRRHDEHPERYTKARHTSIIHEGKEYPAKAIRGIAYEVCFR
                     ERLSQEASSGGQETARFFRRLGFTVVTGSDLSPSPSGR"
BASE COUNT          463 a          745 c          915 g          438 t
ORIGIN      
        1 ataggatgat cgagcggacg cagtgcacct acgtgccgcc tggctcgttt tcatccggtg
       61 aagatgcctc ccgtcggcag cagaggggag acgcccgaat ctctcaggca cgacagacca
      121 cgattggaga cgtcagaatg cacgaatgaa gatcctctca ccctgcgatg acacgagtga
      181 cccggcgatg aagggaagcc aggacggctg ctgtccgatg ggaccatggg atgagagcgg
      241 gttcatcgac catcccagaa cagagccttc gacgtgctgg cccgctttcc tgccggatgt
      301 ggcggaagca acccgttgcg tgctcccgga attggcgtgc cactggagcc ctctcgggcg
      361 agggcgtaac aaaagcgtgt atcctgttgt ctacaggaat gacgacggtt ggcggccgga
      421 ccgcgaccgc cggtgggtgt gtgttgtgtc ggcaggaatg gagcgggtca tgaacgctga
      481 agcaggcgta ctgcaatcgc cggatgagtt ggcgcccgga gggccgctcg tagcgaccgg
      541 cgctgccggc ttgccatctt ggcgggtctt tgccgctgcg acgttggctc tcgccgccgc
      601 ggcggtgtgg ttcacagtgg cgtgtcagga agggcgtttt gcccgtaacg cgatgggtcc
      661 ggatagcgat ctgctatccg cggcagagct ggaggatcag ctgaacattt aggcgtctcg
      721 atggtgtgat actctcttga gagagctcgc agggtccagg catgccaacc gcgggttggc
      781 atgccttccc gcgcctgtcg aactcgatgg aggatcgcat tgacttcata cggccaggca
      841 cagtataatg agggtgtggg cggcatcctg ccgctaccat agatgcgggc ggttggctca
      901 gttggttaga gcggctggtt tacacccagc aggtcgggag ttcgagtctc tcaccgccca
      961 ccaagaaggc cccaggaatg atgctgttcc tggggctttt cactctgcta gccagcggct
     1021 ggctgcccgt cgtgcttcag gtcggagtgc cggcagtccc ccatgccggc tccccaagct
     1081 gtccgcgggc tccaccggtg ctacggagct gaacccgggt ggagatcgca aacggcttcc
     1141 ccggaggtgc ccatctgtgc tggcatgatc ggtgcctggg cacgagccag aacgccacga
     1201 attgcaggat cccaaactca acccaggaga gcacggccgg ggaaaggaac cccttttcga
     1261 ggaggatgac ggatgatggt ggagcgacag actctctacc gttgggtgcg caacggcact
     1321 gtttcgcggt tgcgcgtggc tttgaacgcc ggcctggacc ccaaccaccg caacgctcgc
     1381 cgccggacac tcctacacgt cgctgctgcc agcggccagg cggaggtcgc gcgcttgctc
     1441 ctggaatatg gcgccgtcgt tgacgaccag ccggatccgg ggcaaacccc gctgcttgag
     1501 gccatcgtaa agggccacgc ggaggtgatg cgtgtgctga tcgattatgg ggcagatatc
     1561 tgggtcgagt ccagggaagg catgacgatg ctgcacgccg ctgcgttctg gggagacgag
     1621 gagtgtatcc aggtgctagt ggacgcaggg ctcccgctcg atatggcgga cgacgcaggc
     1681 atgacgcccc tccactacgc ggcaagattc agccccgcat acagggccga gaccgtccgc
     1741 tacctggtgc agctgggagc gcgcgtgaac gtgcgggatc ggagggggca gacgccgcta
     1801 cacttggcgg cctctacgag cgacccggta gtcgtgatgg cgctcctcgc gtgcggggcc
     1861 aacccgcgca tccgggatga tgagggcagg acagcgtggg atgtggctga cgggcgggac
     1921 ggggaggtca tccgggccct gttggaggtc gccctggcca acgaccgcga gtgagcgccc
     1981 cggcggcatg atccgcgacg gcggaggaac cgcgccgggc ccgtggggca gcactggcgg
     2041 cgctgcgtcg gctcgccgat ggccatcgcg acggggtgac cagcgccggt gtgctggatg
     2101 ccctcagccg gcgggggaac gggctgcgtt ccgttggctg ccggacgcgg aaggatcggc
     2161 cgcaaacgcc ctgaacagcg ggtctgggtg caggctggaa gggtgaagcc gggtggacgg
     2221 cacggggccg cagcagccgg atgggtgatg cgccaggggt ggacaggggg agagaggtag
     2281 tcactgatgc caagagatta ccgccgccca cgtcatggaa gcgattcgcc ggcacgacga
     2341 gcatcctgag cgatacacga aggccaggca tacgtctatc atccacgagg ggaaagagta
     2401 tccggcaaag gccattcgcg ggatcgccta tgaggtgtgc ttcagggaga gactcagcca
     2461 ggaagcatcc tcgggaggcc aagagacggc gcgctttttc cggcgcctgg gcttcacggt
     2521 ggtaacgggt agcgatctat ccccttcgcc atctggcagg g
//
LOCUS       DAOSCV010000666         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26396_length_2559_cov_3.760383, whole genome shotgun sequence.
ACCESSION   DAOSCV010000666 DAOSCV010000000
VERSION     DAOSCV010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26396_length_2559_cov_3.760383"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLY56_14715"
     CDS             complement(<1..231)
                     /locus_tag="PLY56_14715"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOJ22777.1"
                     /translation="MADEREPVQEAAAAEAAAEPEPSGAGPRLPPRIVLVQGLLKLPR
                     MDLVVRQATEAGVVAVIPLIAARSLPDVGESGS"
     gene            complement(351..797)
                     /locus_tag="PLY56_14720"
     CDS             complement(351..797)
                     /locus_tag="PLY56_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ22778.1"
                     /translation="MLRPTWDPFTDVIALRDQINRLFDESVSRAPGREGMARRGWAPA
                     VDIFEDNDTITLKMDLPGVQQSDIQVELEGETLTIRGERTLSDQDSPNCLRAERVSGP
                     FLRTFTLATQVQADRVTAQYRDGVLVVTIPKAEETKPRRIQISATA"
     gene            complement(950..>2559)
                     /gene="dnaK"
                     /locus_tag="PLY56_14725"
     CDS             complement(950..>2559)
                     /gene="dnaK"
                     /locus_tag="PLY56_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ22779.1"
                     /translation="MIEIQGKQYTPEEISAMTLQKLKADAEAFLGEKITDAVITVPAY
                     FNDAQRTATKTAGEIAGFNVRRIINEPTAASLAYGLEKKKNETILVFDLGGGTFDVSV
                     LDVGDGVFEVKSTAGDTHLGGDDWDERLVHYLADEFQKMEGVDLRKDRQALQRLREAA
                     EKAKVELSSVTQTNINLPYITATQEGAKHLDLTITRAKFEDLTRDLVHRLEGPFQQAL
                     QDAGLSASQIDEVVLVGGSTRMPMVQEMVRRITGREPHKGVNPDEVVAVGAAIQGGVL
                     AGEVKDVLLLDVTPLSLGIETLGGVFTRLVERNTTIPAHKTEVFSTAADGQTQVTIMV
                     YQGEREMARENRLLGQFDLTGIPPAPRGVPQIEVSFDVDANGILNVSAKDRGTGRQQS
                     ITITGSTSLDKSDVERMVREAAEHAEEDKKRREQAEARNRVDSMSYEAERVLREMGDK
                     IPPQEKGEVQRALDEAREALKGDDMNRISAAGQMLEQALYKATQAAYQAQGGAQGAAT
                     GAGGPQAEATSQGPGEEVIDAEFKETN"
BASE COUNT          365 a          894 c          797 g          503 t
ORIGIN      
        1 ggagccgctt tcgccgacat cgggcagcga gcgggcggcg atcaggggaa tcacggcgac
       61 gacgcccgcc tcggttgcct ggcgcacgac gagatccatg cggggaagct tgagcagccc
      121 ctgcaccagg acgatccgcg gggggaggcg cggccccgct ccggaaggtt ccggctccgc
      181 ggccgcctct gccgccgccg cttcctgaac aggctctctc tcgtcagcca tatccctcta
      241 tcttccttac ctgtgcccgg gggcgcggcg ctgccgcgcg ccccggcacg gatgatgctc
      301 caacaatcgg ccgcatgcct gaatggtgct cagcctctat ggctggaatc ctaagcggtg
      361 gcactgatct ggatgcgacg cggcttggtc tcctctgcct tcgggatcgt caccaccagc
      421 acgccatcgc gatactgcgc cgtcacgcgg tctgcctgta cctgcgttgc cagggtaaag
      481 gtgcgcagaa acggtccaga gactcgctcg gcgcgcaggc agttgggaga atcctggtcg
      541 ctcagcgtgc gctcgccccg gatggtcagc gtctcgccct cgagctccac ctggatatcc
      601 gattgctgaa cgccaggcag atccatcttg agggtaatgg tgtcgttgtc ctcgaagata
      661 tccaccgcgg gcgcccagcc acgccgcgcc attccctcgc gccccggtgc gcgagagacg
      721 ctctcatcga acaaccggtt gatctgatcc cggagcgcga tcacgtcggt aaagggatcc
      781 caagtaggtc gaagcatctt tctctacctc cctcctctca aaacggaacc ggcgcgtgga
      841 tgcaggggta tcccctgctc tatttcgctc catccgcggc ttggggagcg gatcacggac
      901 ggccaggtat cagcccccct gatccgtgtc cccattccgc cgtgtggtgt cagttggtct
      961 ccttgaactc ggcgtcgatc acctcctcac cggggccctg cgaagtcgcc tccgcctggg
     1021 ggccgcccgc gccggtggcg gctccttgtg cgccaccctg cgcctggtag gccgcctgcg
     1081 ttgccttgta gagcgcctgc tccagcatct ggcccgcggc ggagatccgg ttcatatcat
     1141 cccccttcag ggcctctcgg gcctcgtcga gcgcgcgctg cacctcgccc ttctcctggg
     1201 gcgggatctt atcgcccatc tcgcgcaaca cgcgctccgc ctcgtaggac atgctatcga
     1261 cgcggttgcg cgcttcggcc tgctcgcggc gcttcttgtc ctcttccgcg tgctcggcgg
     1321 cttcacgcac catgcgctcg acatcgctct tatccaggct ggtggagccc gtgatcgtga
     1381 tcgattgctg ccggcccgtg ccgcgatcct tcgcgctcac gttcaagatg ccgttggcgt
     1441 cgacatcgaa gctcacctcg atctgcggga cgccgcgggg cgcgggcggg atgccggtga
     1501 ggtcgaactg gcccaacagc cggttctcgc gcgccatctc gcgctcgccc tggtagacca
     1561 tgatggtgac ctgcgtctgg ccatcggcag ccgtcgagaa gacctccgtc ttgtgcgccg
     1621 ggatggtcgt gttccgttcc accaggcggg tgaagacccc gccgagcgtc tcgatgccca
     1681 gggagagcgg cgtcacgtcg aggaggagca catccttcac ctcgcccgcg aggaccccgc
     1741 cctggatcgc ggcgcccacg gccacgacct catcggggtt cacgcccttg tgcggctctc
     1801 tccccgtgat gcggcggacc atctcctgca ccatcggcat gcgcgtggag cctccgacga
     1861 gcaccacctc atcgatctgg cttgcgctca ggcccgcgtc ttgcagcgcc tgctggaagg
     1921 ggccttccag gcggtgcacc aggtcgcgtg tcaggtcctc gaacttggcg cgcgtgatcg
     1981 tgagatccag gtgcttggca ccctcctgcg tcgcggtgat gtaggggagg ttgatgttcg
     2041 tctgggtgac gctggagagc tccaccttcg ccttctcggc tgcctcgcgc aaccgctgca
     2101 gcgcctggcg atccttgcgc aggtccacac cttccatctt ctggaactcg tctgccagat
     2161 agtggaccag gcgctcgtcc cagtcgtcgc cgcccaggtg cgtatcgcca gccgtggact
     2221 tgacctcgaa gacgccgtca cccacatcga gcacggagac atcgaaggta ccgccaccca
     2281 ggtcgaagac gaggatcgtc tcgttcttct tcttctccag gccataggcg agcgatgccg
     2341 ccgtcggctc gttgatgatg cgccggacgt tgaacccggc gatctcaccg gcggtcttgg
     2401 tggccgtgcg ctgggcgtcg ttgaagtagg ccggcacggt gataacggcg tcggtgatct
     2461 tctcacccag gaacgcctcc gcatctgcct tcagcttctg gagggtcatg gccgagatct
     2521 cctcgggggt atactgcttc ccctggatct cgatcatgg
//
LOCUS       DAOSCV010000667         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26429_length_2556_cov_3.665334, whole genome shotgun sequence.
ACCESSION   DAOSCV010000667 DAOSCV010000000
VERSION     DAOSCV010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26429_length_2556_cov_3.665334"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLY56_14730"
     CDS             <1..715
                     /locus_tag="PLY56_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ22780.1"
                     /translation="FDFGERVLLPALLHRGARAIDCVVATHPQSDHIGGLAAVCRELS
                     VGMLIEPDVPSESAEMERLRSEVRRRSIRHLRAQAGVSWMLDPQTRVTLLHPPDPRLP
                     GREDVNDHSVVVRVEYGRASFLLAGDISQDVEAHLLRSGAPLEASVLKVAHHGSRTSS
                     SEAFLDAVRPQIAVVSAGEGNLFGHPATEVIRRLAARAALTCRTDQDGAVEVCTDGEV
                     LDVRLGRHPGAPPWRLLAH"
     gene            874..1653
                     /locus_tag="PLY56_14735"
     CDS             874..1653
                     /locus_tag="PLY56_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ22781.1"
                     /translation="MQAAGKGWAATAVGLFEFIGENGILLGQTLRSLASGTLGGMETL
                     RQMSLIGVNSLPIALITTAFSAAVLSLQSSAQSIKFGMGSLVGGLVSASVAREVAPVL
                     TGVVVAARVGSAIAAELGTMKVTEQVDALRSLAVHPVDYLVVPRFMACVLMLPLLTLY
                     ADAAGVVAGYLVSAGMGVPSGGYFQAATSPVFIRMEHVLLGLAKTVVFGAIIALVSCQ
                     QGLRARGGAAGVGHATTNAVVLSIMLIYVVNYFLSELMFIP"
     gene            1709..2458
                     /locus_tag="PLY56_14740"
     CDS             1709..2458
                     /locus_tag="PLY56_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019551863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22782.1"
                     /translation="MIVVQDLNYKAGGKHILKNVNLTVYVGETLAIMGASGSGKTSLL
                     KIIGGLIRPTSGHVYIGESDISRLSEDRLNRVRLVMGFVFQYAALFDSMTVAENVAFG
                     VRRHFNYSRQQIDRLVEEKLRIVGMEGTGHLLPEQLSGGMQKRVGLARALAMEPLILL
                     YDEPTSGLDPVTTTGIDELTMHLRDAYGVTSVIVSHHIPSIFRVADRIAMIYQGEILA
                     VGTPEEIQKSDNPVVQQFIRGSSQGPIPGVG"
BASE COUNT          403 a          742 c          914 g          497 t
ORIGIN      
        1 gtttgacttt ggcgagcgcg tgctcctgcc ggcgctgctc caccggggcg cccgcgccat
       61 cgattgcgtg gttgctaccc accctcagag cgaccacatc ggcggcctgg ccgcggtgtg
      121 ccgcgagctg tccgttggga tgttgatcga gccggacgtg ccctcggaga gcgcggagat
      181 ggagcggctg cgaagcgaag tgcgcaggcg cagcatccgc cacctgcgcg cccaagcggg
      241 ggtctcctgg atgctcgacc cgcagacgcg ggtgaccctc ctccatccgc cggatccacg
      301 cctgcccggc agagaggatg tgaacgacca cagcgtcgtc gtgcgcgtgg agtatggccg
      361 ggcgagtttc ctcctcgcgg gcgatattag ccaggacgtg gaggcccatc tcctgcgctc
      421 gggggctccc ctggaggcct cggtgttgaa ggtggcccat catggcagtc gcacttcctc
      481 gagcgaggcg tttctggacg cggtccggcc tcagatcgcg gtggtgagcg cgggagaggg
      541 gaacctgttt gggcatccgg cgacggaagt gatccgccgg ctggcggcgc gggcggctct
      601 cacctgccgc accgatcagg atggcgccgt cgaggtgtgc accgatggcg aggtgctgga
      661 tgtgcggctc gggcggcacc cgggggcacc gccctggcgc ttgctggcgc attgatcccg
      721 cgcgccggat tcgacctcct gatcctgctc gggctcgcgt ctcccgctat aatggtcagc
      781 gcttgcctgc cttgacaccg gttcctggaa tcgttatact gtaggcggct agagtgcagc
      841 caatgggtca cgcgttacgt caggatcagg agaatgcaag cagcaggaaa aggctgggcg
      901 gcaacggccg tcgggctttt cgagtttatt ggggagaacg ggatcctgct ggggcagacg
      961 ctgcgctcgt tggcctcagg cacgctcgga gggatggaaa ccctccgaca gatgagcttg
     1021 attggtgtca attccctccc catcgcgctg atcacgactg ccttctcagc cgcggtgctc
     1081 tcattgcagt cctccgccca gtcgatcaag ttcggcatgg gcagtttggt aggggggctc
     1141 gtgtcagcat cggtggcccg agaggtggcg ccggtgctga ccggggtcgt cgtcgccgca
     1201 cgtgtcggct cggccattgc cgcggagctc ggcacgatga aggtgacgga gcaggtagat
     1261 gcgctccggt cgctcgccgt acacccggtg gattacctgg tggtgccacg cttcatggcg
     1321 tgcgtgctca tgctgccgct gttgacgctg tatgcggatg ccgccggggt ggtggccggg
     1381 tatttggtgt cagcggggat gggagtgccc tcgggtggct acttccaggc ggcgacgagc
     1441 ccggtgttca tccgtatgga gcatgtgctc ctggggctcg ccaagacggt ggtgtttggg
     1501 gcgatcattg cgctggtctc gtgccagcag gggcttcgcg cgcgtggagg ggccgcggga
     1561 gtgggccacg cgacgaccaa tgcggtcgtg ttgagcatca tgctgattta cgtcgtcaac
     1621 tacttcctat cggagctgat gttcatcccg taagcgggct cggctccgcc ttgtgagggg
     1681 tgctgccatg gcggggacgc tcgccccgat gattgtggtt caggacctga actataaggc
     1741 gggcgggaag cacattctca agaacgtaaa tctgacggtt tacgtcggag agacgctggc
     1801 gattatgggc gcgtccggct ccggcaagac gtcgctcctg aagatcattg gcggattgat
     1861 ccggccgacc tcgggccacg tctacattgg agagagcgac atcagccggt tgtccgagga
     1921 tcgattgaac cgggtgcgcc tggtgatggg ctttgttttt caatatgccg cgctcttcga
     1981 ctcgatgacg gtcgccgaga acgtggcgtt cggggtgcgc aggcacttca actactcgcg
     2041 ccagcagata gatcgcctgg tggaggagaa gctgcgcatc gtcgggatgg agggcacggg
     2101 ccacctgctc ccggagcagt tgagtggagg gatgcagaag cgtgtgggcc tggcccgggc
     2161 gttggcgatg gagccgctga tcctgctcta cgacgagccc accagcggcc tggacccggt
     2221 gaccaccacg ggcattgatg agctgacgat gcacctgcgc gatgcctatg gagtgacctc
     2281 ggtgatcgtg tcgcaccata tcccgagcat ctttcgagtg gccgaccgga tcgcgatgat
     2341 ctaccagggg gagatcctgg cggtgggaac gccggaggag atccagaaga gtgacaaccc
     2401 ggtggtgcag cagtttatcc gcggcagcag ccagggcccc atccctggtg tggggtagcc
     2461 ccaggggttc aagtgggcgc gcgctcgcga gtgctttaca ttccgcaacc tgagatccga
     2521 agtgcaaagt cggtgagatc ctgatgagcc atgtcg
//
LOCUS       DAOSCV010000668         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26531_length_2549_cov_2.864876, whole genome shotgun sequence.
ACCESSION   DAOSCV010000668 DAOSCV010000000
VERSION     DAOSCV010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26531_length_2549_cov_2.864876"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1327)
                     /locus_tag="PLY56_14745"
     CDS             complement(329..1327)
                     /locus_tag="PLY56_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22783.1"
                     /translation="MMAQLPGLGIVGCGVIAETYLRALKGPCANDWRLAAVCDLDVER
                     ARTVTDGTGASVYGSLEELLADPGVDAVVVLLPNYLHAQATIPALEAGKAVLVEKPMA
                     TTLEDCDRMIATAERTGALLMVGMTSRFRSSYRAAFERLRAGEFGALGLISEYCNYRI
                     RPDWYTRPWLKRAETCGGGMFLQMGIHNLDRAVWLAGSTPQWVHAAIRDRCGNWADET
                     GAATLGLDGGALIQFQTDGLATQGRNETVLHCQDVTVSVNARRITIHRQTEPEVIEFQ
                     EDGFANELREFAAAVREGGPSPLPGAEGRRALALALACYESNRTQAVVRFDAPPWT"
     gene            1685..>2549
                     /locus_tag="PLY56_14750"
     CDS             1685..>2549
                     /locus_tag="PLY56_14750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ22784.1"
                     /translation="MLQYALKELIRRRSRSLLLLFGLIVCTALLAASLSISQAMRNAA
                     RQPFQSANADLVVQKELATCPFALVKHAGKLGGIPWTAVEEIRKLPGVELATGVLELW
                     ATSGPEETDRAVVTGLVPEDQHRIGPVKRGEDCCQLVDHEIGGRYLKEDDDHACLVEK
                     PYAEKHGLGLGGQVTLGGEPFTVVGTLEAAPGARISAGEVYIPLAKAQDLLGEETVNA
                     IMIRAKDQEAAKAIAQELPRIIPVDTAQGEKLVVTSLTRPMSRQPRNASDRSVHPTGM
                     AMDLRRSHRPAC"
BASE COUNT          459 a          858 c          839 g          393 t
ORIGIN      
        1 ccggccctcg gaacccacca gaaccagcgg cgcgagagca tcaacccgcg cccaccgagc
       61 aggcgacgcg cgataggatt gcagccctga tagtcgtaaa gcagccagcc atccaaaccg
      121 gcgcgctcaa tgtggggctg aatgctgttc tccttcatgg ctcctcctga agcactccat
      181 cagagccatt gtacccggtt cggcgccggt tgaaaaaggg agacggcagg ggttcctcac
      241 cgggcgccca tccacgcggc cgcttcgcgc gggcttcacg gggaagagcg ccacgcaggc
      301 acccttcccc acaatcccag gcagaggatc acgtccacgg cggggcatca aagcggacga
      361 cggcctgcgt acggttgctc tcgtaacatg ccagtgccag cgccagagcc ctgcggccct
      421 ctgcgcccgg cagcggtgag ggacctccct cgcgcaccgc ggcggcgaac tcgcgcagct
      481 cgttggcgaa cccatcctcc tggaactcga tcacctcggg ctccgtctgg cggtgaatcg
      541 tgatccgccg cgcgttcacc gaaacggtca cgtcctggca gtggaggacc gtctcattcc
      601 gcccttgcgt cgcaaggccg tccgtctgaa actggatgag ggctccacca tccagcccga
      661 gggtcgccgc gccggtctcg tcggcccagt tgccgcaccg gtcgcgaatc gccgcgtgta
      721 cccattgcgg agtgctcccc gcgagccaaa cggcgcggtc caggttgtgg atccccatct
      781 ggaggaacat gccgccgccg catgtctcgg cgcgcttcag ccacgggcgc gtgtaccagt
      841 caggccggat gcgatagttg cagtactccg aaatgaggcc cagcgctccg aactcccccg
      901 cccgcaagcg ctcgaacgct gcgcgatagg acgagcggaa gcgactggtc atccccacca
      961 tcaggagggc gccagtgcgc tccgcggtgg cgatcatgcg gtcgcaatcc tccagtgttg
     1021 tcgccatcgg cttctcgacg agcactgcct tgcccgcttc gagggccggg atggtcgcct
     1081 gcgcatgcag gtagttcggc aggagcacga cgacggcatc cacaccggga tccgccagca
     1141 gctcctcgag gctgccatag acggaagcgc cggtgccgtc ggtcacagtg cgcgcccttt
     1201 cgacatcgag gtcgcagacg gccgccaggc gccagtcgtt cgcgcacggg cccttcaatg
     1261 cacgaagata agtctcggcg atcacaccgc agccgacgat gccgaggccg ggaagctgtg
     1321 ccatcatggg tgaacccctc tcgctgagtg gtcaaaggga gcgtctagcc ggccactccc
     1381 ctgccctctc ttccccaccg gcagcgcatc tcctgccagg aacgtccgga tcccaggcaa
     1441 cggatagctg gcacgcacgc tgacgatgtg acaggccaag gggaaaggtg gaacgccggc
     1501 gaaaagagag gtgtcccaag cagaagggcg tgcgcgtctt cgcccggttc ttgggggcag
     1561 gatggccggg cgagatccgc ttcgcgggtg gcgcggtagc ctcatcgagc cggcatcgcg
     1621 cgcccgtggg atctgcttcc atgcgcccca ccggggagcc cccacgcagt ggagaacgat
     1681 ccgaatgctt cagtatgcgc tgaaagaact gatccggcgg cgttcacgga gtctgctgct
     1741 cctctttggc ctgatcgtgt gtactgccct gctcgcggcc tctctctcca tctcacaggc
     1801 gatgcgcaat gcggcgcgcc agcccttcca atcagccaat gccgatctgg tggttcagaa
     1861 ggagctggcg acgtgcccct tcgctctggt aaaacacgcc gggaagctgg ggggaatccc
     1921 ctggacggcg gtcgaggaga tccgcaagct gccgggggtg gagctggcta cgggcgtcct
     1981 ggagctttgg gcaacgagcg gcccggaaga gacagaccgc gccgtggtga ccggtctcgt
     2041 gccggaggat cagcaccgga tcgggcccgt caagcgggga gaggattgct gccagctggt
     2101 cgaccacgag atcggcgggc gatatctgaa agaggacgac gatcatgcct gtctcgtcga
     2161 gaaaccgtat gccgaaaagc acggattggg gctaggcggg caggtgaccc tgggcggcga
     2221 gccgttcacc gtcgtcggca cgttggaggc cgcgccggga gcgcgcatct cggcaggcga
     2281 ggtctacatc cccctggcaa aggcgcagga tttgctgggc gaggagacgg tcaacgccat
     2341 catgattcgt gcgaaagatc aggaggcggc caaagcgatc gcgcaagagc ttccgcggat
     2401 catccccgtg gacaccgccc agggcgagaa gctggtggtg acgagcctca cacgaccgat
     2461 gtcgcgccag ccgcgcaacg catccgaccg gtccgttcac cccaccggca tggccatgga
     2521 cctgcggcgg agccaccgcc cggcgtgcc
//
LOCUS       DAOSCV010000669         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26744_length_2534_cov_2.195643, whole genome shotgun sequence.
ACCESSION   DAOSCV010000669 DAOSCV010000000
VERSION     DAOSCV010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26744_length_2534_cov_2.195643"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLY56_14755"
     CDS             complement(<1..207)
                     /locus_tag="PLY56_14755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ22785.1"
                     /translation="MTFQGIDHIALAARNPRALAQWYCDILGFTVVWHNDSEPWAGLI
                     GGPDGSLVEVMPDNGTAPTPHAPFD"
     gene            complement(237..1295)
                     /locus_tag="PLY56_14760"
     CDS             complement(237..1295)
                     /locus_tag="PLY56_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ22786.1"
                     /translation="MTSTERVLAAIAYQPPDRIPLFDSFWAEFVQRWRQEKGLDASAD
                     IEAYYGIDIAICVPDETPWPSQAAELERSETGVVTRDGWGAIHRTRSGAHFYQEAGVA
                     LADKRDPERLEFEAPEADARYAGFLRSLAHARAAGRCPFAKTGGPYLRTMNLRGHEQW
                     LVDIAEDPDYAAALAGRVTDHITAVGVEAIRRGNLQETGIWIYDDIASNLGPMVSPRT
                     YERIFLPLMARMVAAYRAAGARYVVMHSDGNILPVLDMLVDAGIDAINPVEPKAGMDI
                     VALRDRYAGRLAFIGGLDNAGILPSGNRELIREHVTRCIRAARDGGIVLGSHSIGPDI
                     AVADYDYVVELIRSPSGT"
     gene            1509..2516
                     /locus_tag="PLY56_14765"
     CDS             1509..2516
                     /locus_tag="PLY56_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOJ22787.1"
                     /translation="MALYAELHRHLGGSVVPRIFWRYLVRNEHPLAERYPEYEAFEEF
                     FTRPRASLTKYLEMHTLVEQVQSLENLPYFVSKLVRGAYVFESIGYLELRHTPYLRTD
                     PALSEPDRIAQMREVVEVIGVSARVPDYPLVLRQILCMHSRLPYETNRAMVDLAASLP
                     EYVCGVDLAGPDALYEERIGEFVDLYQYARSLGLRTTGHVFETANGCHPELLPYLDRI
                     GHGIQIPLRHPELLPQLAERDQCLEICPTTYLKTGTLQSLSDLRLVFERCRAAGVSVT
                     INTDNAGLHNVRLPWEYENLLTQDVIDFGQLRVCQDAAFRYAFAWPHALPPRAMLPTG
                     Q"
BASE COUNT          428 a          798 c          839 g          469 t
ORIGIN      
        1 gtcgaagggg gcgtgtggtg tgggcgcggt gccgttgtct ggcatcacct cgaccaggct
       61 gccatcgggg ccgccgatga gaccggccca gggctcgctg tcattgtgcc agaccacggt
      121 gaagccgagg atgtcgcaat accattgggc aagggcgcgc gggttacgcg cggcaagcgc
      181 gatatgatca attccctgga atgtcacggg tgttcctttc aggcggaacg cggcgctcag
      241 gttccgctgg ggctgcggat caactcgacg acataatcat agtcggcgac ggcgatatcg
      301 gggccgatgg agtgggaacc gaggacgatg cctccatcgc gggctgcgcg aatgcagcgg
      361 gtgacgtgct cgcgaatcag ctcccgattg cccgagggaa ggattccggc gttgtctaat
      421 ccgccgatga aggcgagccg ccccgcgtag cggtcgcgca gcgcgacgat atccatgccg
      481 gcctttggct caaccgggtt gatcgcatcg atgcccgcgt ccacgagcat atcgagcacg
      541 gggagaatgt tgccatcgct gtgcatgacg acataccggg cgcccgccgc gcggtaggcc
      601 gccaccatgc gggccatcag cggcaggaag atccgctcgt aggtgcgtgg cgagaccatc
      661 ggccccaggt tcgaggcgat atcgtcatag atccagatcc cggtctcttg cagattgccg
      721 cgccggattg cctcgacgcc gaccgccgtg atatggtctg tcacgcggcc ggcgagagcg
      781 gcggcgtagt caggatcctc ggcaatatcg accagccact gctcgtggcc gcgcaggttc
      841 atcgtgcgca ggtagggccc gcccgtcttg gcgaaggggc accgcccggc ggctcgggca
      901 tgggcgagcg accggaggaa gcccgcatag cgcgcatctg cctccggtgc ctcaaactcc
      961 aggcgttcgg gatcccgctt atcggcaagg gcgacgccgg cctcctggta gaagtgggcg
     1021 ccggaccgcg tgcgatggat ggcgccccag ccgtcgcgcg tcacgacccc ggtctcagag
     1081 cgctcgagct ctgctgcctg cgagggccac ggcgtctcat ctggaacgca gatggcgatg
     1141 tcgatcccgt agtaggcttc gatatcggcg cttgcgtcca gccctttctc ctggcgccag
     1201 cgttgcacga actccgccca gaagctgtcg aagagcggga tgcggtctgg gggctgatag
     1261 gcaatggccg cgagaacacg ttcggtggat gtcatcttct tgctcctggt tcctgcccga
     1321 tacccgatcc agttccagct ttctccccct gctcctgccg cggatgatag gcgattgccg
     1381 ccctggagac gcccgcgcgc gttccgcagc cgactggcgt gggtctctgc ttgctgacgc
     1441 gcgccgtcac gttgtggtaa gatggagtca cagccattct gaactggggg agggggaaga
     1501 gtataaggat ggcgctatac gcggagctcc accgtcacct gggcggctcg gttgtcccca
     1561 gaatcttctg gcgttacctg gtgcgcaacg agcacccgct cgcagagcgc tacccggagt
     1621 acgaggcgtt tgaggagttt ttcacgcgcc cgcgggcgag cctgaccaag tatctggaga
     1681 tgcacaccct cgtcgaacag gtgcagagcc tggagaatct gccctacttt gtgagcaagc
     1741 tcgtgcgtgg cgcctacgtc tttgagagca tcggctatct ggagctgcgc cataccccct
     1801 atttgcgcac cgacccggcg ctgagcgagc cggaccgcat cgcacagatg cgggaagtgg
     1861 tggaggtgat cggggtatcc gcgcgcgtgc cggactatcc cctggtgctc cggcagatcc
     1921 tctgcatgca tagccgcctt ccctacgaga ccaatcgcgc catggtcgac ctggccgcat
     1981 ccctcccgga gtatgtctgc ggcgtggatc tggccggccc ggatgcgctc tacgaggagc
     2041 ggatcggcga gttcgtcgat ctctaccagt atgcgcgctc cctgggcctg cggacgaccg
     2101 ggcacgtctt cgagacagcg aacggctgtc atcccgagct gttgccctat ctcgaccgga
     2161 ttgggcatgg cattcagatc ccgctccgcc acccggagct gttgccgcaa ctggcggagc
     2221 gcgaccaatg cctggagatc tgtccgacca cctacctgaa gacgggcacc ctgcaatctc
     2281 tctcggattt acggttagtc ttcgagcgtt gccgggcggc cggggtgtct gtgacgatca
     2341 acacagataa cgcgggcctg cacaatgtgc gcctgccctg ggagtatgaa aacctcctga
     2401 cgcaggatgt gatcgatttt gggcaactgc gcgtctgtca ggatgccgcc tttcgctatg
     2461 ccttcgcgtg gccacatgcg ctgccaccga gagcgatgct ccccaccgga caatagagag
     2521 acaggccgcg gagg
//
LOCUS       DAOSCV010000670         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26753_length_2533_cov_3.650928, whole genome shotgun sequence.
ACCESSION   DAOSCV010000670 DAOSCV010000000
VERSION     DAOSCV010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26753_length_2533_cov_3.650928"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLY56_14770"
     CDS             <1..379
                     /locus_tag="PLY56_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ22788.1"
                     /translation="PDSPYRKSVSKEIVGRGNYDWPGNVRELENCIQRLVTLSSGPVL
                     TTADLPTALAGSLAAGHAARDFEVLLQAPPPETPPVLRLDEMERNAIARALKLTGGDR
                     SRTAELLGIGRTTLYRKIKEYSL"
     gene            473..>2533
                     /locus_tag="PLY56_14775"
     CDS             473..>2533
                     /locus_tag="PLY56_14775"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HOJ22789.1"
                     /translation="MPDKSGPGLGINAWLEDELYQEYLHDRAAIDESWKALFENGHRT
                     NGLAAQVPSARPETAPPPVEGDLEPLRGPAAAVARNMEASLSIPVATSQRTIAVKVVD
                     ENRTIINQHLSVVGQSKVSYTHLIAWAVIKALRAYPNLNSAYVERDGQAFRLRRSYVN
                     FGVAVDVAGKDGARSLVVPNIRNAEKLNFQQFLSAYDDLVARARAGKLTLEDFQDTTI
                     SLTNPGTVGTVGSVPRLMPGQGAIIATGAIDYPAEFQGAAPETRALLGISKVMTITCT
                     YDHRIIQGAESGLFLARVQALLQGDSNFYEDIFADLRLPYQPAKWEPDRSTALPGAGA
                     DQLPAAAKQAAVLQLINAYRVRGHLIADLDPLGSKPSYHPELDPANYGLTIWDLDRHF
                     ITGSLAMDERDGAPYPTATLREILEILRQTYCGKMGCEYMNIQVPEQKRWLQLRLEPA
                     SAKAPLDSATRRRILERLIEAEQFEHFLHSRYVGHKRFSLEGAETAIAILDELLRRAA
                     QQDVHEVVIGMAHRGRLNVLANLIGMPFGQIFSSFESEEPTSVQGSGDVKYHLGATGV
                     RTFEGRELVVSVSPNPSHLEAVDPVVEGIVRPKQDRLGDVERRRVIPLLVHGDAAFAG
                     QGIVAETLNLSQLPGYCTGGTIHLIINNQIGFTTPPEEARSTPYPTDVARMVQAPIFH
                     VNGDD"
BASE COUNT          472 a          902 c          728 g          431 t
ORIGIN      
        1 tcctgattct ccttatcgca aatcagtgtc caaggaaatc gtagggcggg ggaattacga
       61 ctggcccggc aacgtccggg agctggagaa ctgcatccag agattggtga cgctgagttc
      121 cggtccggtt ctgaccacgg ccgatctgcc aaccgccctt gcgggctcgc tcgccgccgg
      181 gcacgccgcg cgcgacttcg aggttctgct ccaggcgccg ccacccgaaa ctccgccggt
      241 gctccggctg gatgaaatgg agcggaacgc gatcgcccgg gcgttgaagc tcaccggagg
      301 cgacaggtcg cgtaccgccg aactgctcgg catcggccgc accaccctct atcgaaaaat
      361 caaggaatac agcctctagt gccgtcgcgg cctggccgga tgcttcccca agcaggagat
      421 aggtatctcc ggcacggtat cataaaagca gacatctaac tgggaattcc ttatgcctga
      481 caaatccggt ccaggcctgg gcatcaatgc ctggcttgaa gacgagctct accaggagta
      541 tctccacgac cgtgccgcga tcgacgagag ctggaaagcc ttgttcgaga acggccaccg
      601 cacaaacggt ctcgccgccc aagtgccgag cgcgagacca gagaccgcgc caccgccagt
      661 cgagggcgat ctcgagcccc ttcgcggtcc cgccgcggcg gttgcccgga acatggaggc
      721 cagcctttcc atccccgtcg cgacttcgca gcgcaccatc gccgtcaagg tcgtcgacga
      781 gaaccgcacg atcatcaacc agcacctatc cgtagtcggg cagtccaagg tctcctacac
      841 gcacctcatc gcctgggcgg tgatcaaggc cctgcgcgcg tacccgaacc tgaacagcgc
      901 ctatgtcgag cgcgacggcc aggcattccg cctgcgccgc tcctacgtca acttcggtgt
      961 ggcggtcgat gtcgccggca aggacggcgc gcggtccctc gtcgttccca atattcgaaa
     1021 tgccgagaag ctgaacttcc agcagttcct ctcggcctat gacgacctgg tcgcccgcgc
     1081 gcgcgccggc aaactcaccc tcgaggattt tcaggacacc accatttcac ttaccaaccc
     1141 cggcaccgtg ggaacggtcg gttccgtccc gcgcctgatg ccggggcagg gcgccatcat
     1201 cgccactggg gctatcgact atccggccga gttccagggc gccgcgccgg aaacacgcgc
     1261 gcttctgggc atcagcaagg tgatgaccat cacctgcacc tatgaccacc gcatcattca
     1321 gggcgccgag tcaggcctct tcctcgcccg tgtgcaggcg ctgctccagg gcgactccaa
     1381 cttttacgaa gacatctttg ccgaccttcg gcttccctac cagcccgcca agtgggaacc
     1441 cgaccgctcc acggccctcc ccggagcggg agccgatcag cttccggccg ccgccaaaca
     1501 ggccgctgtc ctgcaactca tcaacgcata ccgcgttcgc gggcacttga tcgccgacct
     1561 cgatccgctc ggctcgaagc ccagctacca tccggagctc gacccggcca attacggcct
     1621 caccatctgg gacctcgacc gccacttcat taccggaagc ctggccatgg acgagcgcga
     1681 cggcgcgccg taccctaccg ctactctccg ggagattctg gagatcttgc gccagaccta
     1741 ttgcggcaag atgggctgcg agtacatgaa catccaggtg ccggagcaga agcgctggct
     1801 tcaactccgc ctggagccgg cctccgccaa ggcgccgctc gatagcgcca cccgccgccg
     1861 cattctcgaa cgcctcatcg aggccgagca gttcgagcac ttcctgcatt cgcgctacgt
     1921 gggccacaag cgtttctcgc tcgagggggc ggaaaccgcc atagcgattt tagatgagct
     1981 tttgcgccgc gcggcccagc aggatgtcca cgaggttgtc atcggcatgg cccaccgcgg
     2041 ccgcctgaac gtgctggcga acctcatcgg catgccgttc ggacagatct tctcgtcgtt
     2101 tgagagcgag gagcccacca gcgtgcaagg ctccggcgac gtgaagtatc acctcggcgc
     2161 taccggagtc cgcaccttcg agggccgtga gctcgtggtt tcagtttccc ccaacccgag
     2221 tcacctcgaa gctgtcgacc cggtggtcga gggtattgtg cggccgaagc aggaccgcct
     2281 cggagacgtc gagcgccggc gcgtcatccc gctgctcgtg cacggcgatg ccgcgttcgc
     2341 cggccaggga atcgttgccg agaccctcaa cctctcgcag ctcccgggct actgcaccgg
     2401 cggcacgatc cacctcatca ttaataacca gatcggattc acgactccgc ccgaggaagc
     2461 ccgctcgacg ccttacccca ccgacgtcgc ccgcatggtg caggccccca tcttccacgt
     2521 caatggcgac gac
//
LOCUS       DAOSCV010000671         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26883_length_2524_cov_2.984204, whole genome shotgun sequence.
ACCESSION   DAOSCV010000671 DAOSCV010000000
VERSION     DAOSCV010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26883_length_2524_cov_2.984204"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLY56_14780"
     CDS             complement(<1..871)
                     /locus_tag="PLY56_14780"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22790.1"
                     /translation="MKEIGVAIMGAGAVSREHAKAYLKNPHTRIIGVCSRREESAKRL
                     VEELGLECALYPNYEALLSDDRVDAVSICMPNYLHARLATQAARARKHIVLEKPAGIQ
                     LDEVRGLRESVREAGVLSIVSFVFRWNPMVQTIRTLLDQGAIGEIVYAECDFWHGIKP
                     TFPSYEWIRRREFAGGAFITGGCHVVDLIRYCAGEVVEVSARSIRRRPDFDYPTTLVA
                     TLKFSSGAIGKVGCSLEAQCPFTIGVHLLGTEGTIRDNRLYSKRLLPGQTDFATIPTI
                     VPSSGDVRYHPFTA"
     gene            complement(992..1801)
                     /locus_tag="PLY56_14785"
     CDS             complement(992..1801)
                     /locus_tag="PLY56_14785"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ22791.1"
                     /translation="MEKQGDHPLPRVTTADLVRDLRALGLRAGDVVLVHSAMSRIGYV
                     DGGAPAVVQAFLDVLGPEGTLVVPTFPFRGSMLDYCRSGPLFDVQATPSLVGAITEAA
                     RLHPDARRSLEPTHPVAAIGPRAGFLLDDHINAKGSCDEHSPLYRLTQCDGYVLLLGV
                     DFRNCTLLHTAEELARVPFIDFETRYRLRGRDRGRDYVMEIYCHSAPIPARFDAIEPV
                     LREKGLLTLGRVGAAECRLARAKAILDTALAELARDPYFLRARPAEKSAQR"
     gene            2354..>2524
                     /locus_tag="PLY56_14790"
     CDS             2354..>2524
                     /locus_tag="PLY56_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ22792.1"
                     /translation="MARIVGIDLGTTNSVISVMEGGEPTVIPNAEGGRITPSVVAFTK
                     TGERIVGQPAKRQ"
BASE COUNT          457 a          775 c          889 g          403 t
ORIGIN      
        1 cggcggtgaa tgggtggtaa cggacatcgc cgctgctggg cacgatcgtg gggatggtag
       61 caaaatcggt ctggccggga agaagccgtt tcgaatagag gcggttatcg cgaatcgtgc
      121 cctctgtgcc cagaaggtga acgccgatgg tgaagggaca ctgcgcctcc agggagcatc
      181 cgaccttgcc gatggcgccc gaggagaact tcagcgtggc aacgagggtg gtgggatagt
      241 caaagtcggg ccggcggcgg atgctgcgcg cgctcacttc caccacctcg ccggcgcagt
      301 agcggatgag gtccaccaca tggcacccgc cggtgatgaa ggcgcccccg gcgaactcgc
      361 gccggcggat ccattcgtag ctggggaagg tgggcttgat gccgtgccag aaatcgcact
      421 cagcgtagac gatctcaccg atggcgccct ggtcgaggag cgtgcggatc gtctgcacca
      481 tggggttcca gcggaagacg aagctgacga tgctgagcac gccggcctcg cgcacgctct
      541 cccggaggcc gcgcacctca tctagttgga tgcccgcggg tttctccagg acgatgtgct
      601 tccgtgcacg cgcggcctgg gtggccaggc gggcgtgcag atagtttggc atgcagatgg
      661 agacggcatc cacgcggtca tcgctcagca acgcctcgta gttggggtag agggcgcact
      721 ccaggcccaa ttcctcgacg aggcgcttgg cgctctcttc acgccggctg cagacgccga
      781 tgatccgcgt gtgcgggttc ttcaggtagg ccttcgcgtg ctcgcgcgag accgcacccg
      841 cgcccatgat ggcaacaccg atctccttca tctatccatt cccctccagc gccggccggg
      901 gtgatccgtc cggccaggca ggatccagtt cgcgaagcga gtgccggctc cctgccgccg
      961 gtagcctgct gagtggattg gccggccgtg attaccgctg ggcgctcttt tctgcagggc
     1021 gcgcgcgcag gaagtaggga tcgcgagcga gctccgcaag ggcggtgtcg aggatcgcct
     1081 tcgcgcgggc gaggcggcat tcggcggcgc ccacgcgccc aagggtgaga agccccttct
     1141 cgcgcaggac gggctcaatg gcatcgaagc gggccgggat gggcgccgaa tggcagtaga
     1201 tctccatcac gtagtcgcgg cccctatctc ggccccggag gcggtagcgc gtctcgaagt
     1261 cgatgaacgg cacgcgcgcg agctcctcgg cggtgtggag cagcgtgcag ttgcggaaat
     1321 cgacgccgag gagaagcacg tagccgtcgc actgcgtcag ccggtagagc ggggagtgct
     1381 cgtcacacga ccctttggcg ttgatgtggt cgtccaggag aaagccggcg cgcggaccga
     1441 ttgccgcgac ggggtgtgtc ggctccaggc tgcgccgggc atccgggtgc aagcgcgcgg
     1501 cctcggtgat cgcgcccacc agcgaggggg tggcctggac gtcaaacaga gggccgctgc
     1561 ggcagtagtc gagcatcgag ccgcggaagg ggaaggtagg caccacgagc gtgccttccg
     1621 gaccaagcac gtccaggaat gcctgcacga ccgcgggcgc gccgccgtcc acgtagccga
     1681 tccggctcat ggcgctatgc accaacacca catcgcccgc gcgcaagccc agcgcgcgca
     1741 ggtcgcgaac gagatcggcc gtggtcacgc ggggaagagg atgatcgcct tgtttctcca
     1801 tgatgcccct cttcgccgcg ccgcctgtgc ttcccttcgc gcccctcggg gggggcatta
     1861 tctgcggatg aacacagatg gacaccgata gcggaaagac gagccggctt ggctatagga
     1921 aaggccgcgt ccgtgtccgc cccctccatc cgccggcgat ttggtagcgg gggcctggga
     1981 gggggagcca cgaacgggct ttgcctacaa gcagcgcgga gcctgggctc cgcgcgagag
     2041 gtgtggtgga cacccccgaa gggatctcac actcccggga gcccccaccc ggcccatcca
     2101 tccccttcac gcgcggtatc accgccaggc tgcaccaggg ttgtcctgct ggaatctggg
     2161 tagtaaagaa agagtgcgtg ccccatcgca gggatgttga caacaccatt atcaagtgct
     2221 atactaagcc tagactaaag atcagatcgg gcgaatcccg gggcaaggga cgagctgtat
     2281 cagggccggg gagccggtag gttgatgagg ggaaagtggg cggagaacgc ccgcgcgaag
     2341 aggtgagaag actatggcaa gaatcgtggg aatcgatctg gggaccacca actccgtgat
     2401 ctcggtcatg gaaggtggcg agcccaccgt cattccgaat gccgagggcg ggcgaatcac
     2461 gccttccgtg gttgcgttca cgaaaacggg agagcgcatc gtcggtcagc cggctaagcg
     2521 gcag
//
LOCUS       DAOSCV010000672         2523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26900_length_2523_cov_2.964749, whole genome shotgun sequence.
ACCESSION   DAOSCV010000672 DAOSCV010000000
VERSION     DAOSCV010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26900_length_2523_cov_2.964749"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1475)
                     /locus_tag="PLY56_14795"
     CDS             complement(183..1475)
                     /locus_tag="PLY56_14795"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ22793.1"
                     /translation="MAENRPIQIGILGLGNVGSGVVRVLESNAEAIAHKAGGPIQVKR
                     ILVRDVAKARLVEIPPSLLTTDAQEILADPEIDIVCELIGGVEPAHRYVVEALERGKQ
                     VVTANKELMARHGHAILAEAARHRRDVYFEAAVAGGIPLIRPLKVDLAGNRIARISGI
                     LNGTTNYILTRMAAEGKEFSVALREAQELGYAEADPTYDIEGLDAAYKIAILAAIAFQ
                     TPVDVTRVYHEGITRISPRDIAHARELGYVIKLLATARDEGSAIDVRVHPALVPQNHP
                     LASVNDAFNAVLLNGDAVGDVMFYGRGAGSLPTGSAVVGDLIDVARNIRFGATGRVPC
                     VCNRNKPMRDIAELSTENYMRMLVKDQPGVIAAIAGVLGESRVSIDAVVQKGTEGDLA
                     EIVWITHPAPESSFRGALTRIAQLPVVHEICNVIRVER"
     gene            complement(1479..1919)
                     /locus_tag="PLY56_14800"
     CDS             complement(1479..1919)
                     /locus_tag="PLY56_14800"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22794.1"
                     /translation="MEENRPVLDAETVRTLVRLVEENALAELTVEDVDFAITLKAEGI
                     LQSVLLPAAQPRQEEGETVEEGHAFVRAPMTGVFYRAPAPKEPPFVEVGDHIEVGQTI
                     GLIEAMKVFSEVPSEVAGRVVDIPAKNGELVQQDQVLVVVAVEE"
     gene            complement(1934..>2523)
                     /locus_tag="PLY56_14805"
     CDS             complement(1934..>2523)
                     /locus_tag="PLY56_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22795.1"
                     /translation="AFAALGLLDDLRGSRAHTGLRGHVTALVRERKVTTGLLKLVGGA
                     ATGVAIAWLARPRFPEALLDGALIALAANLINLLDLRPGRALKGFALGALALVPAAGF
                     PGMLPLVAVMLPAAGYAPADLRARMMMGDSGSNALGAALGVTAVLHLEWGARLAILTL
                     LLGLHLASERVSFSRVIERHPVLRCLDQLGREPRS"
BASE COUNT          447 a          867 c          780 g          429 t
ORIGIN      
        1 cactggcggt ggagccattg agcgcggagc tgaagctccg cgctgcttct gagcgacggc
       61 ccttcgggcc ttcgccggtc ccaccgcccg ctcgcattgt ccggggcctg accaggctag
      121 cccggcaggg cttagcctcc aagcagcgcg gagattaatc tccgcgcgac cggatcgccc
      181 gttcaccgtt cgacgcggat gacgttgcag atctcgtgga ccacggggag ctgggcaatg
      241 cgagtgagcg cgccgcggaa actcgactcc ggcgccgggt gggtgatcca gacgatctcc
      301 gccaaatcgc cctccgtgcc cttctgcaca accgcgtcga tgctcacgcg gctttcgccg
      361 aggacgccag cgatggcagc gatcacccca ggctggtcct tgacgagcat gcgcatatag
      421 ttctcggttg agagctcggc gatgtcgcgc atcggcttgt tgcggttgca tacgcaaggc
      481 acgcgcccgg tagcgccgaa gcggatgttc cgggccacat cgatcaggtc gccgacgacg
      541 gcgctccccg tcggcagcga accggcaccg cggccgtaga acatgacgtc gcccacggcg
      601 tcgccgttga ggagcacggc gttgaaggca tcgttcaccg aggcgagtgg atggttttgg
      661 ggaaccagcg ccggatgaac gcgaacatcg atggcgcttc cctcatctcg ggcggttgcc
      721 aggagcttga tgacgtagcc gagctcgcgc gcgtgcgcga tatcccgggg ggagatccgc
      781 gtgatcccct cgtggtagac ccgggttaca tccaccggcg tctggaacgc gatggcggcg
      841 aggatggcga tcttgtaagc ggcatccagg ccttcgatat cgtaggtggg gtccgcctcg
      901 gcgtagccca attcctgcgc ttcgcggagt gccaccgaga actccttgcc ctcggcggcc
      961 atgcgcgtca ggatatagtt cgtggtgccg ttgaggatgc cggagatgcg ggcgatgcga
     1021 ttgcccgcca gatccacctt cagcgggcga atcagaggga ttccgccggc aaccgcggcc
     1081 tcgaaataga cgtcacgccg atgccgcgcc gcctcggcga ggatggcgtg tccatgccgg
     1141 gccatcagct ccttattggc agtgacgacc tgcttgcctc gttcgagtgc ctcgacaacg
     1201 tagcggtgcg ccggctccac gcccccgatc agctcgcaca caatgtcgat ctcgggatcc
     1261 gccaggatct cctgcgcatc cgtcgtcagc agggaggggg ggatttccac caggcgtgct
     1321 ttcgccacgt cgcgcaccag gatccgcttg acctggatcg gcccccctgc tttgtgcgcg
     1381 atggcctccg cgttgctctc caacacgcga acgacgccgc tgcctacgtt gccaagaccg
     1441 agaatcccaa tctggatcgg ccgattttct gccatccgct actcctcaac cgccacaacc
     1501 accaacacct ggtcctgctg aaccagctcc ccattcttcg cgggaatatc gaccactcgg
     1561 ccagcaacct cggaaggaac ctcactgaag accttcatcg cctcgatcag gccgatcgtc
     1621 tggccgactt caatgtggtc gcccacctcc acgaagggcg gctccttggg cgctggcgct
     1681 cgatagaaga cgccggtcat cggagcccgc acgaacgcgt gcccctcttc aacggtttcg
     1741 ccttcttcct gtcgaggctg tgccgccggg agcagtacgc tctggagaat gccctccgct
     1801 ttcagggtga tcgcgaagtc gacgtcctcc accgtgagtt ccgccagggc gttctcctcg
     1861 acgaggcgca cgagcgttcg cacggtctcc gcgtccagca ctggtcgatt ctcctccatg
     1921 aagacctctc atctcatgat cgcggttccc ggcccaattg atcgaggcac cgaagcaccg
     1981 ggtgacgctc gatcacccgt gaaaacgaga ccctctcgct ggcaagatgc aagccgagaa
     2041 ggagcgtgag gatcgccaaa cgcgcgcccc actccaggtg cagcaccgcc gtcactccca
     2101 gggcggcacc cagggcattc gaaccgctat ctcccatcat catacgcgcg cggagatccg
     2161 ccggcgcgta acccgcagcc ggaagcatca ccgcgactag cgggagcatg ccgggaaaac
     2221 cagcagccgg caccagcgca agcgcgccca gggcaaaccc cttcaatgcc cttcccgggc
     2281 gcaggtccag caggttgatc agatttgccg cgagagcaat caaggcgcca tctagcaacg
     2341 cctctgggaa gcgcggtcgc gccagccagg caatggctac tccagtggca gcgccaccaa
     2401 cgagcttgag cagtccggtt gtcaccttac gctcgcgcac cagcgccgtc acgtgcccgc
     2461 ggaggccggt gtgggcgcgg ctgccgcgca ggtcatccaa gagacccaag gccgcgaagg
     2521 cgg
//
LOCUS       DAOSCV010000673         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_26979_length_2517_cov_2.973193, whole genome shotgun sequence.
ACCESSION   DAOSCV010000673 DAOSCV010000000
VERSION     DAOSCV010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26979_length_2517_cov_2.973193"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLY56_14810"
     CDS             complement(<1..337)
                     /locus_tag="PLY56_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-mannanase"
                     /protein_id="HOJ22796.1"
                     /translation="MEIDLTKPSEFVVGCNYWASHAGTRMWAEWDPEVVERDFRLLSD
                     AGLQLLRVFPLWPDFQPIHLLRGGGGRPVEFRHGEDPLPDDPLGQAGLSHQAMEQFAT
                     FLDLAEKYRL"
     gene            complement(658..1581)
                     /locus_tag="PLY56_14815"
     CDS             complement(658..1581)
                     /locus_tag="PLY56_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ22797.1"
                     /translation="MKRVWIDTHIHVSDLGPDGQRRERMLEHLLDVLDRCDADLRFII
                     SCDTPYFSAMTRDPDAILAGNRFIYELVRQAPGRLFGSCMVNPHFQKESLRAMQICFE
                     EWGFVMLGEMLQYSMNYRMESDLVEPLLRLAARYDVPVQVHLGTYWTRNSRDRSTDGL
                     DHIRGLIQAAERVPEAKYILAHAIGCGPTPDYIPWANMFLDTLAGFFPSFPDNFWVEI
                     RDFHCPALARTLAEVPATRILAGTDWTNRVGPPFQAYGTMFGVSESENPFPPRVSSFV
                     EFLRKAGASEEVIDRIGSRNATELFRLPATA"
     gene            complement(1797..2300)
                     /locus_tag="PLY56_14820"
     CDS             complement(1797..2300)
                     /locus_tag="PLY56_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ22798.1"
                     /translation="MRKMTEENLKAAFAGESQAHMKYLNYAEKAKREGLENVARLFAA
                     ASYAEQVHASAHLRVLEGIGSTAENLADAASGEGFESDEMYPAYIAVAEAQGEKSANR
                     VFSAALEAEKVHRALYLRAKESVDAGRDAEIGPIWVCSVCGFTGEGDAPDNCPVCKAV
                     KDRFNIF"
BASE COUNT          424 a          798 c          868 g          427 t
ORIGIN      
        1 tcaggcgata cttctccgcc aggtccagga aggtggcgaa ttgctccatc gcctgatgcg
       61 agaggcctgc ctgcccgagg ggatcatccg gaagagggtc ctccccatgt cggaactcga
      121 ccggacgccc gcccccaccg cgcaggaggt ggatcggctg gaaatcgggc cagaggggaa
      181 agacgcgcag gagctgcagt ccggcgtcgc tgaggaggcg gaagtcgcgt tccacgacct
      241 ccgggtccca ttcggcccac atgcgcgtgc ctgcgtgcga ggcccagtaa ttgcacccga
      301 cgacgaactc gctaggcttc gtcagatcga tctccatctc catccgcgtc cttccgtgct
      361 cagggatacc ggcttccaag gggggagacg cgggcgagca agcggcccaa tgagaggcac
      421 cgcgcgccgg gcggcccagg cgccccgtca cgccttcgcc ccgccgttct cctttcgacg
      481 ttgccgggtc ggtttcacgt tcccgacggc gcgcccgatt ccctgccatg agccggcgtg
      541 caggaggggg aagcgcctgc gtgacgagca ggaccgttac ccgcgtcggg caggagtgac
      601 gacggatgcc tggcggcaaa tcgcctgttc aaaggacgct cgccctgggg gcgagcgtta
      661 cgccgtagct ggcaggcgga acagctcggt ggcgttgcgc gaaccgatac ggtcgatcac
      721 ctcttccgag gccccggcct tccggaggaa ctcgacaaag gaagagacgc gcggcgggaa
      781 gggattctcg ctctcgctca cgccgaacat cgtgccgtat gcctggaacg gagggccgac
      841 gcggttcgtc cagtcggtgc cggcgaggat gcgggtcgcg ggcacctcgg caagcgtgcg
      901 cgccagcgcc ggacaatgga agtcgcggat ctctacccag aagttgtcgg gaaagctcgg
      961 gaagaagccg gccagggtgt ccaggaacat gttggcccag gggatatagt ccggcgtggg
     1021 cccgcagccg atggcgtgcg ccaggatata cttcgcttct ggaacgcgct cggccgcctg
     1081 gatcaggccg cggatatgat ccagaccgtc ggtggagcga tctcggctgt tgcgggtcca
     1141 gtaggtgccg aggtgcacct ggaccggaac atcatagcgg gcggcgaggc ggaggagtgg
     1201 ctccacgagg tcgctctcca tgcggtagtt catgctgtac tggagcatct cgccgagcat
     1261 gacgaagccc cactcctcga agcagatctg catggcgcgc agcgactctt tttggaagtg
     1321 ggggttgacc atgcagctcc caaagagccg gcccggcgcc tggcgcacca gctcgtagat
     1381 aaagcggttg cctgccagaa tcgcgtccgg gtcgcgggtc atcgcagaga agtagggagt
     1441 gtcgcagctg ataatgaagc gcaggtcggc atcgcagcgg tcgaggacgt cgagaagatg
     1501 ctccagcatg cgctcgcggc gctggccatc tggcccgagg tcactcacgt ggatgtgggt
     1561 atcgatccac actcgtttca tctggcacct cgcttcgcgc gtcgtgctcc tgccgctgga
     1621 atggagcgtc agggggccgt ggaatcgccg gggcttcgct gcgaagggga tggatgcaac
     1681 gcgagtggcg tcgcggctcc cggctatcgt gcccaccagg tgatcagggg gcaatggggc
     1741 aggggcgcga ggcgagcccc tggtgtctcg ccccgcgcag catctgcgtg catcggttag
     1801 aagatgttga accggtcctt caccgccttg cacaccgggc agttatcggg ggcgtcgccc
     1861 tcgccggtga agccgcagac ggagcaaacc cagatcgggc cgatctcggc gtcgcggccg
     1921 gcgtccacgg actccttggc gcgcaggtag agggcgcggt gcaccttctc ggcctccagg
     1981 gcagcgctga agacgcggtt ggcgctcttc tcgccctgag cctcggccac agcgatgtag
     2041 gcgggataca tctcgtcaga ctcaaacccc tcaccagagg cggcatccgc caggttctcc
     2101 gcggtgctgc cgatgccctc gagcacacgc aggtgggcgg atgcgtggac ctgctccgcg
     2161 taggacgcgg cggcaaagag gcgtgccacg ttctccaggc cctcgcgctt tgccttctcc
     2221 gcgtagttca aatacttcat gtgtgcctgg ctctcgccgg cgaaggccgc cttcaggttc
     2281 tcttcagtca tcttccgcat gtcaatcctc cttctgataa tgcaatacgg gtgcgccggg
     2341 cacccgaaag tcaggaatag ctccgtccgg tagaggacag cgttcccagc agcagatcgc
     2401 tcttcaggtg aggtgctatg ggcgcctttg cctgatgggc gtggctggct agcggcaccg
     2461 cttgcgacgc ggcgggatgc ccgcacgtgg ccagggcgcg gcgccccgaa cccacct
//
LOCUS       DAOSCV010000674         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27078_length_2511_cov_3.592020, whole genome shotgun sequence.
ACCESSION   DAOSCV010000674 DAOSCV010000000
VERSION     DAOSCV010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27078_length_2511_cov_3.592020"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..429)
                     /locus_tag="PLY56_14825"
     CDS             complement(217..429)
                     /locus_tag="PLY56_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22799.1"
                     /translation="MTTGTVVVVAIVVAYVVFMVVVKILPRVRNIGKIYRSAAVTRPW
                     NGGEWTGLPPGEIPPGSRTRVLTTDG"
     gene            complement(491..1792)
                     /locus_tag="PLY56_14830"
     CDS             complement(491..1792)
                     /locus_tag="PLY56_14830"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ22800.1"
                     /translation="MADIAPFAGLRYNLPQVGGLQQVVAPPYDVITPDEQAALYERHP
                     ANVVRLILGKELPGDDGQNRYTRAAACLEEWLRDGVLLREKDPALYLCRMEYEDAEGA
                     FRALTILYCQLRLEPLGAGGVLPHEATLSGPKEDRLRLLRATRTNLSGVFCLVSDPNA
                     VLEQLCAERTARPPLGSATDIWGVRHRLWVETDPSIAAAARAFLQEKPLFIADGHHRY
                     ETALRYRDEVRAANGNRPGPWDWTLVAIAALESPGITVLPTHRVVREVAPAQLNTFLE
                     RARDLFAVEPAGDLSELLACMRDEQAAGRHAFGVFHADEGFHLLRLTAPERADALLDP
                     VRAPAWRQLDVALLHGIIIEHLLGVSSEQAMQSGQIGYLKDAAQCVRQVEAGEFQAAL
                     FLNPTPPEAVKEVALAGEKMPQKSTYFFPKLLSGLVMRPVY"
     gene            complement(1847..>2511)
                     /locus_tag="PLY56_14835"
     CDS             complement(1847..>2511)
                     /locus_tag="PLY56_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22801.1"
                     /translation="PLAEILLDARRMVAAAEKKQVLLAMNAQYVAGLPAYMEWYAQVR
                     GPLDRPSRYEAVMQSRGRGGGAEYRDIWIDLGSHPLSLIVKWSPGVHIEPGSIHCVLR
                     RKEVVADFRCVAANGNVCDCSIRLGNVPEGKLERRFGVNGFLLDYEGRNNPEGVFHTY
                     LSHGDEEREYPDLLLMSIEAFVRAVRGEGEPLISGAEGLRNLELQVEILEHAVVEQGA
                     AD"
BASE COUNT          451 a          838 c          787 g          435 t
ORIGIN      
        1 tcggtcccga agcgcccggt ctccgggtgg taggtatcca ggatcttcgg cacctcttcc
       61 acgaggctct tgagaaggta ctcgcgtggg ggccattcct gccacggcga agccgcggcg
      121 tccacggtag cgccaaacaa cagtacggcg atgagagcgg agatctgcag catcgttccg
      181 tcccttcagg acatgaggga aaggagcttt cctggatcac ccgtcggtgg tcagcacgcg
      241 agtacgcgaa ccgggtggaa tctcccctgg gggtaggcct gtccattccc caccattcca
      301 ggggcgcgtg accgcggcgc tacggtagat cttcccgatg ttcctcacgc gcggcaggat
      361 cttcaccact accatgaaga ccacgtaggc gacgacaatg gcgaccacga ccacggtgcc
      421 cgtagtcatc catccctcgg gctgccacca gacacttttc gtcttcttgt ctcttgggct
      481 cggcattagt ttagtatact gggcgcatga ccagcccgct caagagcttc gggaagaagt
      541 aggtggattt ctgaggcatc ttctcgcccg cgagcgccac ttccttcacc gcctccggtg
      601 gcgttgggtt caggaagagc gccgcctgga actcgcctgc ttccacctgg cgcacgcatt
      661 gcgccgcgtc tttcagatag ccgatctggc cggactgcat cgcctgctcg gaggaaaccc
      721 cgaggagatg ctcaatgatg atgccatgca gaagcgccac gtccagctgg cgccacgcag
      781 gcgcgcgcac cggatccagg agcgcgtccg cccgttctgg cgcggtgagg cgcaggagat
      841 ggaagccttc gtcggcgtgg aagacgccga aagcgtgccg gccagctgcc tgctcatcgc
      901 gcatgcacgc caggagctcg ctcagatccc ctgcgggctc cacggcaaag agatcgcgcg
      961 cccgctccag gaaggtgttc agttgggccg gcgccacttc gcgtaccacg cgatgggtgg
     1021 ggagcaccgt gataccgggg ctctccaggg cggcgatagc taccagggtc cagtcccacg
     1081 ggccgggccg attgccattg gcagcgcgca cctcgtcgcg atagcggagc gccgtctcat
     1141 agcgatggtg gccatcggcg atgaagagag gcttctcctg caggaaggca cgcgcagcgg
     1201 cggcgatgga ggggtcggtc tccacccaca gccggtggcg tacgccccag atatcggtgg
     1261 cgctaccgag gggcgggcgc gcggttcgct cggcgcagag ctgctcgagc accgcgtttg
     1321 gatcggagac gagacagaag acgccgctca ggttggtccg ggtggcccgg agaaggcgca
     1381 agcggtcctc ctttgggcca gaaagcgtcg cctcgtgggg aagcacgccc cctgcaccaa
     1441 gaggttccag gcgcagctgg cagtagagaa tggtcagcgc acggaaggcc ccctcggcat
     1501 cctcgtactc catgcggcag agatagagcg cgggatcctt ctcgcgcagc agcacgccat
     1561 ccctcagcca ctcctcgagg cacgcagcag cgcgcgtgta gcggttctga ccatcatcgc
     1621 ccggcagctc tttcccgaga atgagccgga ccacgttggc aggatgccgc tcgtagagcg
     1681 ccgcctgctc atcgggcgtg atcacgtcgt aggggggcgc aaccacctgc tggagcccgc
     1741 ccacttgcgg gagattatag cgcagacctg cgaatggtgc gatatctgcc atcaagcctt
     1801 ccttgcatca agattgaaac tgccgcctgg cccaaagtat atcgggtcaa tcggctgcgc
     1861 cttgctccac caccgcgtgc tcgaggatct ccacctgcag ctccaggttg cgcaggccct
     1921 cggcccccga gatgagcggc tcgccctcgc ctcgcacggc acggacgaac gcctcgatac
     1981 tcatcaggag cagatcaggg tactcccgct cctcatctcc gtggctcaga taggtatgga
     2041 acaccccctc ggggttgttg cgtccctcgt agtccagcaa gaagccgttg actccgaagc
     2101 ggcgttccag cttcccctcg ggcacgttgc cgagccggat agagcagtcg cagacgttgc
     2161 cattggccgc cacacatcgg aaatcagcca ccacctcttt gcggcgcagg acgcagtgga
     2221 tgctccccgg ctcgatgtgg acgccggggc tccatttcac gatcagactg agcgggtgag
     2281 acccaaggtc aatccagata tccctatatt cggcgccacc gcctcgcccg cgcgattgca
     2341 tcacggcctc gtagcggctc ggacggtcga gcgggccgcg cacctgcgca taccactcca
     2401 tgtaggccgg cagccccgcg acatattgcg cattcatggc cagcagcacc tgcttcttct
     2461 cggctgcagc gaccatgcgc cgcgcatcca gcaagatctc cgcgagcggc t
//
LOCUS       DAOSCV010000675         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27107_length_2509_cov_3.309291, whole genome shotgun sequence.
ACCESSION   DAOSCV010000675 DAOSCV010000000
VERSION     DAOSCV010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27107_length_2509_cov_3.309291"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1856
                     /locus_tag="PLY56_14840"
     CDS             <1..1856
                     /locus_tag="PLY56_14840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22802.1"
                     /translation="LAMPRAHAVEKPAMRIAVVPAIPAAAGGVLLPDRLSAGVADLFA
                     RSLSQIRGVTVSSSSAVYHAVLAAGGSGGSTPVAAGLAARRVQADLAVLVEAKADGAV
                     LKITGTPVLPESGRGKAPAPFTSQGTRALETDLFSIQAGFIKAGLEALGLKPTPQEQA
                     RIDLVARPTVSPEAFQLYCEARQHLPIASVEAYDRAIKVLRLIVVGDGPSRPPVDPEF
                     ALAHAALAEAAALGSMQQWLVGLRDQRLMALALSSAETAVKLRPELPDSHRALALAGA
                     LVKRISVAVNAARKALEIHPGDPQSHLWLGVANGGDGAPEINAALALDPSLGLGHLFL
                     AVLAAAEGDQKRMLVECERALELSPVDASPLLLMGMMQTGEGKLDEAIATYQRVLKIN
                     RHAGLAHGALARLYFRKGQTGLAQDALRQAFALDYRVAELMREQAAAYQQAGKLEEAE
                     KEYRLILLGDDKDTPSLRGLGMLALERGKVDEAEKLLRRALQISPKDAWSFVGLSRCH
                     YRQGKRDEALQDLRRAAEVQPDDGFVQLELGQAYEQHNRTREALEAYDKAIRLLRLPD
                     AYFRKAQLLAKSDPARARDAWKGFLEAIEAMGGPASESDRVRVEMAKRALQ"
     gene            1931..2338
                     /locus_tag="PLY56_14845"
     CDS             1931..2338
                     /locus_tag="PLY56_14845"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ22803.1"
                     /translation="MRRLEQERRGQAVVELALVLSFLLLTASAVVQFGTLYGIKLRVE
                     HAAREGARYGAIHAITGTDEEIRAHTIAVAHDLQPAIRADNIAIETPEGRGAKKPIVV
                     TVVYPYSPTVPLISELLPETINIRATVQMRIEG"
BASE COUNT          406 a          812 c          872 g          419 t
ORIGIN      
        1 ggttggcgat gccacgtgcc catgcggtgg agaagccggc gatgcgcatc gccgtcgtgc
       61 ctgccatccc cgccgcggcc ggaggcgtct tgcttcccga ccgtctctct gctggcgtgg
      121 ccgacctctt cgcacgatcg ctcagccaga tccggggcgt caccgtctcc agctcgagcg
      181 ccgtctacca tgccgtgctg gcagctgggg gctctggggg aagcacacct gttgccgcag
      241 ggctcgcggc ccggcgcgtg caggccgacc tcgccgtgct cgtagaagcc aaggcggatg
      301 gcgcggtgct gaagatcact ggcacgcccg tgctcccgga gagtgggcgt ggcaaggccc
      361 ccgctccatt cacctcgcag ggcacgcgcg ctctggaaac ggatctcttc agcatccagg
      421 cggggtttat caaagccggc ctcgaggcgt tgggcctgaa gcccacgcca caggagcagg
      481 cgcgcatcga tctggtggcg cgcccgaccg tgtcgcccga ggcgtttcag ctctactgcg
      541 aagcccgcca gcacctccca atcgcttccg tggaggccta cgaccgcgcc attaaggtgc
      601 tgcgtctgat tgtggtcggt gatggcccca gccggccacc cgtcgatccc gagtttgccc
      661 tggctcatgc cgccctcgcc gaggcggccg ccctgggaag catgcagcag tggctcgttg
      721 gcctgcgtga ccagcgcctg atggcactcg cgctctcctc cgcggaaacc gcggtgaagc
      781 tgcgcccgga gctgccggac tcgcaccgcg cgctggcgct cgctggcgcg ctcgtcaaac
      841 gcatcagcgt ggccgtgaac gctgcgcgca aggcgcttga aattcatccg ggcgatcccc
      901 agtcccacct ttggctgggt gtagccaacg gcggcgatgg cgcccctgag atcaacgccg
      961 cgctggcgct cgacccgtcg ctgggcctcg ggcacctctt tctggccgtt ctggccgccg
     1021 ccgagggcga tcagaagcgc atgctggtcg aatgcgagcg agccttggag ctgagcccgg
     1081 tcgatgcctc cccgctgctc ctcatgggga tgatgcagac gggcgaggga aagctcgatg
     1141 aggcgatagc cacctaccag cgcgtcctga agatcaaccg gcacgccgga ttggcccatg
     1201 gcgccctggc gcgcctctac ttccgaaagg gtcagacggg gctggcacaa gatgccctgc
     1261 ggcaggcctt tgccctggat taccgtgtcg cagagttgat gcgcgagcag gcagccgcct
     1321 accagcaggc gggtaaactg gaggaggcag agaaggagta ccgtctgatc ctgctaggtg
     1381 atgataaaga cacgccatcg ttgcgcggtc tcggaatgct ggcgctggag cgcggcaagg
     1441 tagacgaggc ggagaagttg ttgcgccggg cgctccagat tagccctaag gatgcgtggt
     1501 cgttcgtggg gctctcacgc tgccactacc gccagggcaa gcgagatgaa gcgcttcagg
     1561 atttgcgccg cgctgcagag gtgcagccgg atgatggctt tgtgcagctg gagctaggac
     1621 aggcctacga gcaacacaat cgaacgcgcg aggcgctgga ggcctacgac aaggcgatcc
     1681 gcctgttgcg cctgccggat gcttacttcc gcaaggcgca actgctggcc aagagcgatc
     1741 cggcacgcgc gcgcgatgcc tggaagggct tcctggaggc catcgaagcg atgggcggtc
     1801 ccgcgtcgga gagcgaccgg gttcgcgtcg agatggcgaa gcgggccttg cagtagctag
     1861 cggcgcgcgg tattcatccc ggggccggcc ggatcatctc ctggcaggcc ttcatgaagg
     1921 tgagcttgcg atgaggcgct tggagcaaga gcggaggggg caggcggtcg tcgagctagc
     1981 gctcgtcctg tcgttcttgc tcctgactgc gtcggcggtc gtgcagttcg gaacgctcta
     2041 cgggatcaag ctgcgcgtgg aacacgctgc gcgggagggc gcccgctacg gcgcgatcca
     2101 cgccattacg ggcacggacg aggagatccg ggcgcacacg atcgccgtgg cgcatgacct
     2161 ccagccggcg atccgggccg acaacatcgc catcgagacg cccgagggcc gcggggccaa
     2221 gaagccaatt gtcgtgacgg tggtctaccc gtactcgccc accgtgccgc tgatcagcga
     2281 gctcctgccg gagactatca acattcgggc aaccgtgcag atgcggattg agggctaaac
     2341 gtgagcgggc ggcacacacg agggttagtc cgtcgaggag gatgcgcgcg cgggcaggcg
     2401 ctggtcgagt tcgccctctg cgccagcatc ttcctgttgc tgacgttcgc cgtgatgcag
     2461 tttgccatcc tggggcatat ccgcctggtg ctcgggcacg ccagccgcg
//
LOCUS       DAOSCV010000676         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27109_length_2509_cov_2.896903, whole genome shotgun sequence.
ACCESSION   DAOSCV010000676 DAOSCV010000000
VERSION     DAOSCV010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27109_length_2509_cov_2.896903"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLY56_14850"
     CDS             complement(<1..825)
                     /locus_tag="PLY56_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ22804.1"
                     /translation="MVRVAIIGCGAFAESQHIPNCVAHPDVEVVALCDLRRERLEEVA
                     RRFGLERASLVTDSAEVFRRSDVDAVLAATDHSAHLPLIAEAARSGKHLLIEKPMTMT
                     QEESLQALRLVKQSGIKLCVDYNRPFSPSMRAIKEALEEHRTDPVTSPWRSRRVSGFP
                     TLPEERATNLVITINDEIDSYRPQHLDPTRGGGQILGESCHWFDLACWLIDRRPVRVL
                     ASGSTRLSHSVTLDFEDGSMATLFFSVCGTFDYPKERYELTSGGALFLNEFFVETAV"
     gene            complement(913..>2509)
                     /locus_tag="PLY56_14855"
     CDS             complement(913..>2509)
                     /locus_tag="PLY56_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22805.1"
                     /translation="VQNYPVEGVWIDILYLPWHKTDLQEEVCFCEHCHRQYSEWFHGE
                     HLVDAAGTPRHDEFRALTYRNFLARLKEMLLAQDRPLLLTFNGAGRRRMPGYKICDDL
                     ADLLSGEAHNPLSLSITSRSHRNDGRPFELLSCAEVCWSHNQMKPETLIRLESLSTLI
                     AGGTYTIGITHAPDGRLSPANINRLERWSRWIHERASLFQESEPVCEVGILAPELGPT
                     GIETWASWLRQGHFQFNVFMDLNAALGYPPAPEGLGRYRLIVVPHARTLSEEEARRLR
                     EYVEGGGHLLLEYPAAALRDHLPWIDELAGVRQLREPRAYSFYLLPDAPELAAGLPEG
                     EPVLYYVNSYACLELTTGEAMGHVVPQFADKVRTSDIQTVANYWARPDQAPHHPGLIL
                     NRVGKGRVLLSVLPLTAPNRDSDRCPWPRILALNCVRSLLGQPLVSLRGCGQVEVFLS
                     RQPDRWLLHFLNHAYGAGEAIPGEGEEEFLREIPVRLAPWLREAVTAASLEPEGTPVA
                     LTAEGFVLPRLGVYQAVALKEER"
BASE COUNT          424 a          797 c          877 g          411 t
ORIGIN      
        1 gaccgccgtc tcgacgaaga actcgttcag gaagagcgcg ccgccgctgg tgagctcgta
       61 gcgctccttc ggatagtcga aagtgccgca gacggagaag aagagggtgg ccatcgagcc
      121 atcctcgaag tcgagcgtca cggaatggct gagccgggtg gagccggagg cgagcacgcg
      181 cacgggccgg cggtcgatga gccagcacgc gagatcgaac cagtggcagc tctcgcccag
      241 gatctgcccg ccgccgcgcg tgggatcgag gtgctggggg cggtaggaat cgatctcgtc
      301 gttgatcgtg atcaccaggt tggtggcccg ctcctctgga agcgtgggga atccggaaac
      361 gcgcctgctt ctccaggggc tcgtcaccgg gtcggtccgg tgctcctcca gcgcctcttt
      421 gatcgcccgc atcgagggcg agaagggccg gttgtaatcg acgcacaact tgatcccgct
      481 ctgcttcacc aggcggagcg cctgcagcga ctcctcctgt gtcatcgtca tcggcttctc
      541 gatcaggagg tgcttgccgc tgcgcgccgc ctccgcgatg agcggcaggt gcgccgaatg
      601 gtcggtcgcc gccaggaccg cgtccacgtc ggaacggcgg aaaacctcgg cgctgtcggt
      661 cacgagcgag gcccgctcta gcccgaaccg gcgggccacc tcctccaggc gctcccggcg
      721 gaggtcgcac agggcgacga cctccacatc cggatgcgcc acgcagttgg gaatgtgctg
      781 tgactcggcg aatgcgccac acccgatgat tgctactcgg accatctata aatcacctgt
      841 tctagctcta gtgcgattcg gggagagtag gcctcccctg ggaatgaact ggtttggcga
      901 gggggtctgc cgctagcgtt cctccttcag agcgaccgcc tgatagacac ccaggcgtgg
      961 cagcacgaaa ccctcagccg tgagcgcgac cggcgtgcct tccggctcca gcgacgctgc
     1021 ggtgaccgcc tctcgcagcc agggcgcgag gcgcactggt atctcgcgaa ggaactcctc
     1081 ctcgccctca ccggggatcg cctccccggc gccgtaggcg tgattcagga agtgcagcag
     1141 ccatcgatct ggctgtcgcg acaggaaaac ctccacctgg ccgcaccctc tcagcgagac
     1201 gagcggctgg cccaggagcg agcgcacgca gttgagcgcg agtatccgcg gccacgggca
     1261 gcggtcagag tcgcggttcg gcgcggtgag ggggagcacc gagaggagca cgcggccttt
     1321 cccaacccgg ttgagaataa gccccgggtg gtgcggggct tggtcgggcc tcgcccagta
     1381 gttggccacg gtctggatgt cggaggtgcg caccttatcg gcgaactggg gcaccacgtg
     1441 gcccatcgcc tcgccagtgg tcagctcgag gcacgcgtag gagttgacgt aatagagcac
     1501 cggctcgccc tcgggcaatc cggcggcaag ctccggcgca tctggcagca ggtagaagga
     1561 gtaggcgcgc ggctcgcgaa gctgccggac gccagccagc tcatcgatcc aggggaggtg
     1621 gtcgcgcagc gccgcagccg gatactccag gaggaggtgg ccgccgcctt caacgtactc
     1681 gcggagccgg cgtgcctctt cctcggagag agtgcgtgcg tgcggcacca cgatgagccg
     1741 gtagcgcccc agaccttcgg gcgccggcgg gtagccaagg gcggcgttta gatccatgaa
     1801 gacgttgaac tggaagtgac cctgacgcag ccacgaagcc cacgtctcga tcccggttgg
     1861 cccgagctcg ggtgccagga tacccacctc gcagaccggc tcgctctcct ggaagaggga
     1921 tgcgcgctcg tggatccacc ggctccagcg ctccagacgg ttgatgttcg cgggtgagag
     1981 acggccgtcg ggggcgtgcg tgatcccgat ggtgtaggtg ccgccggcga tcagcgtgga
     2041 aaggctttcc agccggatga gcgtctcggg cttcatctgg ttgtgcgacc agcagacttc
     2101 agcgcagctc aggagctcga acggccgtcc gtcgttgcga tggctccggg atgtgatgct
     2161 gagcgagagc gggttgtgcg cctcgccgga gaggaggtcg gctaggtcgt cgcagatctt
     2221 gtagccgggc atccgccgcc ggccggcccc gttgaacgtt aggagcaggg ggcgatcctg
     2281 cgccaggagc atctccttca gccgtgcgag gaagttgcgg taggtgagcg cgcgaaactc
     2341 atcatgccgg ggcgtgccag cggcgtcaac caggtgctcg ccgtggaacc actccgagta
     2401 ctgccggtgg cagtgctcgc agaagcagac ttcctcctga aggtcggtct tgtgccacgg
     2461 caggtagagg atgtcgatcc agacgccctc caccgggtag ttctgcacc
//
LOCUS       DAOSCV010000677         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27139_length_2507_cov_3.307504, whole genome shotgun sequence.
ACCESSION   DAOSCV010000677 DAOSCV010000000
VERSION     DAOSCV010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27139_length_2507_cov_3.307504"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PLY56_14860"
     CDS             complement(<1..893)
                     /locus_tag="PLY56_14860"
                     /inference="COORDINATES: protein motif:HMM:NF025465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HOJ22806.1"
                     /translation="MVRITVSAGNHDRIFTPVSADLPAQGDTALELIDQESQRAVPFQ
                     LDATGTRITWLIDHLAAGQSRTYALCPASSTAVPPATVSLTEVPNDRVEVRIGDELIT
                     SYYYGSAYPRPFLYPLIGPYGASVTRHFPMQNIEGERQDHKHHRSVYTAYGDVNGVDD
                     WSEEPGHGRIVHTGFQGLEQGPVYATIRSQSDWVSNSGEKVMSESRAVTIYGVPSAAR
                     ILDYEVRLTATVGEVKFGDTKEGGILSVRVATSMDAARGGKIENSYGGVNEKETWGKR
                     AEWCDYSGPVEGKWVGIAVMD"
     gene            complement(1042..1860)
                     /locus_tag="PLY56_14865"
     CDS             complement(1042..1860)
                     /locus_tag="PLY56_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002650722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ22807.1"
                     /translation="MKFAICNEMFQGWKIDDVLQYAARIGYDAVEIAPFTLAESVEQI
                     PASERQRIREAAAAAGVEIAGIHWVLVKPEGLYINTSDEAVRQRTADYFVALTHFCAD
                     LGGKVMVVGSPKQRNVMEGVSYEQAWKWAAETFRPAVRVAEERGVVICFEPLAPSETN
                     FINTAADAIRFVQQVPSPAFRIILDVKAMSSEGKPIPQIIRESQGWFSHFHANDPNLK
                     GPGMGDMEFVPIFRALKEANFDGYVSVEVFDFSDGPETIATRSLEYMREAMSQA"
     gene            2139..>2507
                     /locus_tag="PLY56_14870"
     CDS             2139..>2507
                     /locus_tag="PLY56_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ22808.1"
                     /translation="MAHKVTLIPGDGTGPEIVAAGRRVLDATGVAIDWDVQEAGVDVM
                     EKHGTPLPPEVLESIRRNKVALKGPITTPIGTGFRSVNVALRQELGLYACLRPCKYYP
                     GIRSKYTDVDLVVVRENTEDL"
BASE COUNT          441 a          779 c          823 g          464 t
ORIGIN      
        1 tgatccatga cggcgatgcc gacccacttg ccctcgaccg gtccggagta atcgcaccac
       61 tcggcgcgct tgccccaggt ctccttctcg ttgacgccgc cgtagctgtt ctcgatcttg
      121 ccgccgcgcg ccgcgtccat cgaggtcgcc acgcgcacgg agaggatgcc gccctctttg
      181 gtatcgccga acttcacttc ccccacggtg gcggtgaggc gcacctcata gtcgagaatc
      241 cgcgctgcgg aaggaacgcc atagatggta accgcgcggc tctcgctcat caccttctcg
      301 ccggagttgc tcacccagtc gctctgcgag cggatggtgg cataaacggg gccctgctcc
      361 aggccctgga agccggtgtg cacgatccgg ccatgcccgg gctcctccga ccagtcatcc
      421 acgccgttca cgtcgccgta ggcggtgtag accgagcggt ggtgcttgtg gtcctggcgc
      481 tcgccctcga tgttctgcat cgggaagtgg cgcgtcacgg aggcgccgta ggggccgatg
      541 agcgggtaga ggaacgggcg gggataggcg ctgccgtagt agtaagaggt gatcagctcg
      601 tcgccgatgc gcacttccac gcggtcgttg ggcacctcgg tgaggctgac cgtggccgga
      661 ggcacggcgg tgctggatgc ggggcagagg gcgtaggtgc gcgactgccc tgccgcgagg
      721 tggtcaataa gccaggtgat gcgcgtgccc gtcgcatcaa gctggaacgg caccgcgcgc
      781 tggctctcct ggtcgatcaa ctccagggcg gtgtcgccct gcgcgggcag atcggcgctc
      841 acgggggtaa agatccggtc gtggttgcct gctgaaacag tgatacggac catctctgtt
      901 gctccttagt aagataggct actgtcgcaa caacgcttcc agaaggagaa gcgatggcga
      961 acgcgcttga acgcggcgcc agtgcggatt ctggcggcct cgttcccccg cgcgggtcac
     1021 cgcttctctg tcatggacgc gctacgcctg gctcatcgcc tctcgcatat actccaggct
     1081 gcgcgtggca atcgtctctg ggccatcgct gaaatcgaac acctcgacgg agacgtagcc
     1141 gtcgaagttg gcctccttca gggcgcggaa gatggggacg aactccatat cgcccatgcc
     1201 gggccccttc aggttcgggt cgttcgcgtg gaagtgggag aaccacccct gcgactcgcg
     1261 gatgatctga ggaattggtt tcccctcgga actcatcgcc ttcacatcga ggatgatccg
     1321 gaaggcgggg ctgggcacct gctgcacaaa gcggatggcg tcggcggcgg tgttgatgaa
     1381 gttcgtctcg gagggggcaa gcggttcgaa gcagatcacc accccgcgct cttcggcgac
     1441 gcggacggct ggccggaacg tctccgcagc ccacttccag gcctgctcgt agctcacgcc
     1501 ctccatcacg ttgcgctgct tgggagagcc aacgaccatc accttgccgc ccaaatcggc
     1561 acagaaatgg gtgagcgcga cgaaatagtc ggcggtgcgc tgacgaacag cctcgtccga
     1621 ggtgttgatg taaaggccct ccggcttgac gagcacccag tggatgccag cgatctccac
     1681 ccctgcggca gccgccgcct cgcggatacg ctggcgctcg gaagcgggaa tctgctccac
     1741 gctctcggca agggtgaagg gggcgatctc gacggcatca tagccgatgc gcgccgcgta
     1801 ctgcagcacg tcgtcgatct tccaaccctg gaacatctcg ttgcagatag caaacttcat
     1861 ggcgcctcca tatcgggccg cagccgttga ttgctgaagt gagaagggag ggcgcgtccc
     1921 gtaagccccc gcgcctctca agcgtagcca gcgcgcggca gcctccggta ttatagcagc
     1981 taagccgaat gcccgcaagc aggagagcag gccctgggtt gcgaagtagg tgagggaatg
     2041 cgaagaccgg gcgtgtggtt tcgcttcttc aggcgcgcca tctcacagtg ggcgcccagg
     2101 caatccgcaa tctgctgctt agttgggagg aaactcggtt ggcacataag gtcacgctga
     2161 ttccgggaga tgggacgggc ccggagattg ttgcggcagg gcgccgcgtt ctcgatgcaa
     2221 cgggtgttgc gattgattgg gatgttcagg aagcaggcgt tgatgtcatg gagaagcatg
     2281 gcacgccgct gccgcccgag gtgctggaga gcatccgccg caataaggtc gcgctcaagg
     2341 ggccgatcac cacgcccatc ggcaccggtt tccgctcggt gaatgtggcc ctgcgccagg
     2401 aacttggcct ctacgcctgc ctgcgcccct gcaagtacta tcccggcatc cgatcgaaat
     2461 acaccgatgt cgatttggtg gtcgttcgcg agaacaccga ggatctt
//
LOCUS       DAOSCV010000678         2497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27290_length_2497_cov_3.589681, whole genome shotgun sequence.
ACCESSION   DAOSCV010000678 DAOSCV010000000
VERSION     DAOSCV010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27290_length_2497_cov_3.589681"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLY56_14875"
     CDS             complement(<1..388)
                     /locus_tag="PLY56_14875"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOJ22809.1"
                     /translation="MYTSNLDLQTTVRDSFTLRGVGIHSGEAVAVTVQPACEGGIRFH
                     RADQPAVSIPAHLSSLQATPRCTVLAAGEIRVATTEHLLSALWALGVDHARVLVEGDE
                     LPALDGSALEYVQALQRVGLRDLDQPR"
     gene            complement(686..>2497)
                     /gene="fusA"
                     /locus_tag="PLY56_14880"
     CDS             complement(686..>2497)
                     /gene="fusA"
                     /locus_tag="PLY56_14880"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ22810.1"
                     /translation="RSALSVVEGAIVLVDAVAGVEVGTELYWRLAEQNNLARIFFINK
                     MRRENANFEGVVEGIRTRFGKAAVAVQVPVGSELNFRGVVDIVSDKAYTWNEGKEPQE
                     GPVPAELADTVAEYRNTLIEALADVDEELGDKFLMEEEITREDIIRGIRKGVRERTLF
                     PILLGAGADNIGVQPLLDLIVECVPSPLDRGTVTGKHPDTGEEEKRQTGMDAPFSAYV
                     FKSTADPYVGKLNFFRVWSGTLRADSSVYDATRKHDERIGQILIMRGKHQEPVPQVNA
                     GDIAAVAKLQHVHTGDTLADKSKPIVYPQVEYPEPVMFYAVEPKTKGDEDKLGAGLAR
                     LADADPAFRTYRDTVTKETIIAGMGDQHLAVVVDRLQRMGVAVQTREPRVAYQEIIKK
                     TVEQEYKHKKQTGGRGQYGHVVLRISPAPGEGFKFGDEIVGGVVPKQFIPAVQKGVEE
                     ALAEGVLAGYPVTDVEVVLTFGSYHTVDSSELAFKIAASQAFKEGVLKANPSIQEPIY
                     DVEITVPEENMGDVISDLNGKRGRVQGMDQIAPGTQLIRAQVPLGELHRYSIDLRSIT
                     RGRATYTMKFSHYEEAPANVVQQIIEEAKRAKEEEKK"
BASE COUNT          423 a          826 c          794 g          454 t
ORIGIN      
        1 ctcgcggctg atccagatct cgcaggccga cgcgctgtaa cgcctgcaca tactcgaggg
       61 cgcttccatc caaggccgga agctcatcgc cctctaccag gacgcgggcg tgatcgaccc
      121 ccaacgccca aagagcgctc aggagatgct ccgtcgtcgc cacgcggatc tcgccggcag
      181 cgagcaccgt acagcgaggc gtcgcctgca gtgacgaaag atgcgccggg atgctgacgg
      241 ctggctggtc ggcacgatgg aagcggatgc ccccttcgca cgccggctga actgtgaccg
      301 ccaccgcttc gccggaatgg atgcccacgc cacgcagggt gaaggagtcc cgcacggttg
      361 tctgtagatc gaggttgctt gtgtacacca ggcgtcatta taacatattc acgcggggga
      421 cggaagcccc cgtgcgagat cgtcgcgccc agggtctcca gtgggacgca ggggccagct
      481 ctcccacgcc gcagcccgag ctggcggcga tactacggat gaagggaaca gacgcggatg
      541 ggaaggtgca ggcctcgcgg atctcccgcc cgctggggcc ggaagcgcga agcaatacag
      601 tgcggggcga tacggaacgc cgcctcggga agagcgaagg gggtgggccg ccccgcggcc
      661 cacccccttc gctgaagtag caagactact tcttctcctc ttccttcgcc cgcttggcct
      721 cctcgatgat ctgctggacc acgttcgcgg gggcttcctc gtagtgggag aacttcatcg
      781 tgtaggtggc acgaccgcgc gtaatggagc gcaggtcgat gctataacga tgcagctcgc
      841 ccaggggcac ctgagcccga atgagctgcg tgcccggggc gatctggtcc atgccctgaa
      901 cccgaccgcg ctttccattg aggtcagaga tcacgtcgcc catattctcc tcgggaacgg
      961 tgatctcgac atcgtagatc ggctcctgga tgcttgggtt cgccttgagc acgccttcct
     1021 tgaacgcctg cgaagcggcg atcttgaacg cgagctccga ggagtccacc gtgtggtagg
     1081 agccgaaggt aagcaccact tcgacgtcgg tcacgggata gccggccaga acgccttcag
     1141 cgagcgcttc ctcgacgccc ttctggacgg cggggatgaa ctgcttcggc acgacgccac
     1201 ccacgatctc atcgccgaac ttgaagccct cgccgggcgc cggcgagatg cgcagaacga
     1261 cgtgcccgta ctggccgcga ccgccggtct gcttcttgtg cttatactct tgctcgacgg
     1321 tcttcttgat gatctcctga taggccacgc gcggctcgcg cgtctggaca gcaacgccca
     1381 tgcgctggag gcgatccacg accaccgcca ggtgctggtc gcccatgccg gcgataatcg
     1441 tctccttggt caccgtgtcg cgataggtgc ggaacgccgg gtcggcatcg gccaggcgag
     1501 caaggccagc gcccagcttg tcctcatcgc ccttggtctt cggctccacc gcgtagaaca
     1561 tgaccggctc gggatactcc acctgcggat agacgatcgg cttggactta tcggcgaggg
     1621 tatctccggt atgcacatgc tgcagcttcg ccaccgcggc gatgtcgccc gcgttcacct
     1681 gcggcaccgg ctcctggtgc ttgccgcgca tgatgaggat ctggccgatg cgctcgtcgt
     1741 gcttgcgcgt ggcatcgtag acgctggaat cggcgcgcag cgtgccggac cagacacgga
     1801 agaagttgag cttccccacg tagggatcgg cggtggactt gaagacgtag gcggagaatg
     1861 gggcgtccat gcccgtctgg cgcttctctt cctccccggt atccgggtgc ttccctgtca
     1921 ccgtcccgcg gtcgaggggg ctggggacgc actcgacgat cagatcgagc agcggttgaa
     1981 cgccgatatt gtcggcgcct gcgcccagga ggatcgggaa gagggtgcgc tcgcgaacgc
     2041 ccttccgaat gccgcggatg atgtcctcgc gggtgatctc ctcctccatg aggaacttgt
     2101 cgcccagctc ctcatccaca tccgccagcg cctcaatcag ggtgttgcgg tattccgcga
     2161 cggtatcggc cagttcagcc ggcacggggc cttcctgggg ctccttgccc tcgttccagg
     2221 tgtaggcctt gtcgctgacg atgtcgacga cgccgcggaa gttcagctcg gagcccaccg
     2281 gcacctgcac ggccacggcc gccttgccga agcgcgtgcg gataccctcc accacgccct
     2341 caaagttggc gttctcgcgg cgcatcttgt tgatgaagaa gatgcgcgcc aggttgttct
     2401 gttctgccag gcgccagtag agctcggtgc ccacttccac tccggccacg gcatccacga
     2461 gcacgatcgc cccttcaaca acactcaggg cgctgcg
//
LOCUS       DAOSCV010000679         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27418_length_2489_cov_3.395645, whole genome shotgun sequence.
ACCESSION   DAOSCV010000679 DAOSCV010000000
VERSION     DAOSCV010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27418_length_2489_cov_3.395645"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLY56_14885"
     CDS             complement(<1..239)
                     /locus_tag="PLY56_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22811.1"
                     /translation="MKRRRGAEATALALTLGLGGAIALGVVRLQPALAAGTVLCLEAE
                     AGKLTAPVTLYKSNDCSGAAAIEVPEGANPKAEEG"
     gene            complement(375..1715)
                     /locus_tag="PLY56_14890"
     CDS             complement(375..1715)
                     /locus_tag="PLY56_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22812.1"
                     /translation="MSEKQQRERGLFPILLLMILVAPTQWGLTIKGIPVAPADLVLGV
                     GFLVWAVLALRKQVRPAMPPACLWMLVAVALASAVNAIRAVNTGQGFVFMAPGYEIAG
                     TPLKSVAEVVQLVEYFLVAYVLAFSAVREERHARVAGLALAAVTALVVLGGLFHYVTR
                     ADEPFFVRSSFGSRGIYGGYLAMTLPVLFAMALYSVDTRLRLGLFAVVIAGAVTILSP
                     GALIGLVTALLLVAGRSSSRVLQATLAGVLAFGVVTAIALPRNYDQNVYQFLHFRTAD
                     EQQLRAQWREWLAGVFITQEHPLLGVGPGNFQQNLANAYGAHGLEYPGKARPGADLAS
                     GLMEPDTNNLYVVTAATTGLLGLAALLWMLIYFGKAASAVVRRPGSGVARVLALGIWG
                     GIIGFAVTSVFSSLLVRGTGIVLAIFFALAAVLYECRWPASETTEGGAPELRVV"
     gene            complement(1955..>2228)
                     /locus_tag="PLY56_14895"
     CDS             complement(1955..>2228)
                     /locus_tag="PLY56_14895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ22813.1"
                     /translation="PYAPLEAMRAGIPVVASDLPPLREMVVHQQTGLLVPPDDPEALA
                     GALSSLLSAPEWARALGRAGQARVCERFSLRRQVEQLALVYRTLAR"
     assembly_gap    2229..2238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2239..>2489)
                     /locus_tag="PLY56_14900"
     CDS             complement(<2239..>2489)
                     /locus_tag="PLY56_14900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22814.1"
                     /translation="RLVEQKAPDVLVRAAALVLARHPDALFLLAGAGPHFETVQRQVA
                     RWLAEGPPGINPERVRLLGHRQDIERFYAAAQIFVLPSR"
BASE COUNT          446 a          880 c          792 g          361 t
ORIGIN      
        1 ttgccctctt cggcttttgg gttcgcgcct tccggtacct cgatcgcggc ggccccggag
       61 caatcgttgc tcttgtagag ggtgacggga gccgtcagct tgccggcctc ggcctccagg
      121 cagaggacgg tacccgccgc gagcgccggc tgcaggcgga caacacccaa cgcgatggct
      181 ccaccgagcc ccagggtgag agccagcgct gtggcttcgg cgcctcttcg tctcttcatc
      241 ccctttgttc ctccttccat cagaaatgaa acatgctcct aatcaaccat aggtcgtcgt
      301 tgatgcccaa attgttcatg cggtgccaaa ccaccgttgc gtgcggagcg cgcaggctgc
      361 atccagcccc gctgctacac cacgcgaagt tccggcgcgc ccccttccgt ggtctcggac
      421 gcgggccacc ggcactcgta gagcaccgcc gccagcgcaa agaagatcgc caggacgatc
      481 cctgtgccgc gcaccaggag cgagctgaag acgctcgtca ccgcgaaccc gatgatcccg
      541 ccccagatgc cgagggccag cacccgggcc actccgctcc cggggcgccg gacgacggcg
      601 gaagccgcct tcccgaagta gatcaacatc cagaggagcg ccgccagtcc caggagaccg
      661 gttgtcgctg ccgtgacgac atacaggttg ttcgtgtcgg gctccatcag tccactcgcc
      721 aggtcggccc cagggcgggc cttgccgggg tattcaagcc cgtgcgctcc atacgcgttg
      781 gcgaggttct gctggaagtt gcccgggccc acgcccaaca gcggatgctc ctgcgtgata
      841 aagacgccgg cgagccactc gcgccactgc gcccgcagct gctgctcgtc cgccgtgcgg
      901 aagtgcagga actggtagac gttctgatca tagtttctgg gcagggcaat cgccgtcacc
      961 accccgaacg ccagcacgcc cgcgagcgtg gcctgcagca ccctgctcga gcttcggccc
     1021 gcgaccagca ggagcgcggt gaccaatccg atcaacgcgc caggcgagag gatcgtgacc
     1081 gcgccggcga tgacgaccgc gaacagcccc aggcgcaatc gggtgtcgac cgagtagagc
     1141 gccatcgcga agagaaccgg gagcgtcatc gccagatagc cgccgtagat cccgcggctt
     1201 ccgaagctgc tgcgcacgaa gaacggctca tcggcgcgcg tgacgtaatg gaagagaccc
     1261 cccagcacca ccaacgccgt gactgccgcc agcgccaatc cagcgacacg cgcgtgccgc
     1321 tcttcccgca cggcagagaa ggccagcacg taggccacca ggaagtactc aaccagttga
     1381 acaacctcgg ccaccgattt gagtggcgtg ccggcgatct cgtagccggg cgccatgaag
     1441 acgaagccct ggcccgtatt aactgcccgg atggcattca ccgcgctggc gagagcgacg
     1501 gccacgagca tccagaggca cgccgggggc atcgccgggc gcacctgctt ccggagagcc
     1561 agaaccgccc agaccagaaa gcccacgccc agaaccaggt cggctggggc gaccgggatg
     1621 cctttgatgg tcagacccca ctgcgtgggc gccacgagta tcatcaggag caggatcggg
     1681 aagagcccgc gctctcgctg ttgcttctca gacatccgct ttcgctttcc aggcgcgctt
     1741 ccgcccgaag cgggaacacg ccacgccaga acaaatggcc ctcgggccat agcgcccctt
     1801 gccggagccg gacccgcggc aaggcgagat cagaagcgcg ttttgccatt gccgcgcggc
     1861 caccccgcac gtgaaggggc atcttctctt tagacccttt ccgggcgctt tcgtttcgct
     1921 tgcgtgccat tctcgggccc gcgcggccca tgcgctaccg cgcgagagtc cgatacacca
     1981 gggcgagttg ctccacctga cgccggagcg agaagcgctc gcacactctc gcctggcctg
     2041 cgcgccccag ggctcgtgcc cattcggggg ccgagagaag cgatgagagc gcgccggcca
     2101 gcgcctccgg gtcatcgggg gggaccagca ggcccgtctg ctggtgaacg accatctcgc
     2161 gcaggggcgg gaggtcgctg gcaacaaccg gaatcccagc gcgcatcgcc tctagcgggg
     2221 cgtagggann nnnnnnnngc gcgagggaag cacgaagatc tgcgcggccg cgtaaaaccg
     2281 ctcgatatcc tggcggtggc ccagcagtcg aacgcgctcc gggttgatcc ccggcgggcc
     2341 ctcggcgagc caccgggcca cctgccgctg caccgtctcg aagtgcggac cagcgccagc
     2401 gaggagaaag agcgcgtccg ggtgcctggc cagcacgagc gcggcggcgc gcaccagtac
     2461 gtcgggcgct ttctgctcca ccaaccggc
//
LOCUS       DAOSCV010000680         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27547_length_2480_cov_3.688247, whole genome shotgun sequence.
ACCESSION   DAOSCV010000680 DAOSCV010000000
VERSION     DAOSCV010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27547_length_2480_cov_3.688247"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="PLY56_14905"
     CDS             <1..1496
                     /locus_tag="PLY56_14905"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ22815.1"
                     /translation="LIDDPAATARRLAGPLDRSPEYLEKCLRREGSFAWLRRKVDRET
                     EQAVRALNLKGIDFVPETKRAYPLKSVAANLIGFTDIDGKGVEGLEQQLQETIAGKPG
                     WVKAEVDALGRIIPGTTYERVEAVDGHSVILTIDARIQHLAETAIAKAQATYQAAGVS
                     AVVLDPRTGEILALANRPTYDPNMPAKSETASRRNRCVTDLYEPGSTLKAITVAAALE
                     EGVIRPTSLFHCAGSYPIGKQRIRCVVHAPYTNGHGAQTIRGVLRESCNIGTAQIASR
                     LGSRKLYRYIADFGFIGDTRSGLIGERRGSLTPPSTWAPIKQANVSFGQGVSVTALQV
                     AVAYGAIANGGKLMQPYFIRRIEDGEGKVVQEYHPSVVRQVISKQTADLLAECLQEVV
                     EKGSGKPAQLEGYTVAGKTGSAQKVIEGWRGYAPGRFVASFIGFAPVSEPRIVCIVVV
                     DEPKGSHWGATCAAPAFREIVQGALWCMKVPPDRPVDLQQTALGMQR"
     gene            1743..>2480
                     /locus_tag="PLY56_14910"
     CDS             1743..>2480
                     /locus_tag="PLY56_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOJ22816.1"
                     /translation="MTLNLKAIIEGLSIQQQAGPAVPPAGGTNVPVVAGIAYDSRKVE
                     PGALFVCIRGYKTDGHLYIEDALARGAAAIVVEQASAIPANPPVPFLLVPNARRALAT
                     LSRNAHGAPSRNLRLVGITGTNGKTTTTYMVRHILFHAGRPTGVIGTLGALIGDRPVP
                     LDRTTPEAPELDRILAEMVAAGVQDCAMEVSSHAIALHRVRELEFDIGVFTNLTQDHL
                     DFHKSLEDYRAAKMEIEVVLREVGEDAD"
BASE COUNT          445 a          805 c          822 g          408 t
ORIGIN      
        1 cgctgatcga tgacccggcg gcgacggccc gccggctcgc agggcccctg gaccggtcac
       61 cggagtacct cgagaagtgt ctccggcgcg agggctcctt tgcctggctg cggcgtaaag
      121 tcgatcggga gaccgaacag gcggtgcgcg cgctcaacct caaggggatc gacttcgttc
      181 cggaaacgaa gcgggcgtat ccactcaaga gcgtggccgc caacctgatc ggcttcaccg
      241 atatcgacgg gaagggcgtc gagggcctgg agcagcagct ccaggagacc atcgccggca
      301 agccaggatg ggtgaaagcg gaagtggacg cgctcgggcg gatcatcccg ggcaccactt
      361 acgagcgcgt ggaagcggtg gatggccaca gcgtcatcct gaccattgac gcgcgtattc
      421 agcacctggc ggaaaccgca atcgccaagg cgcaggcgac ttaccaggcg gctggtgtca
      481 gcgccgtcgt cctcgacccg cgcacgggcg agatccttgc cctggcgaat cggccaacct
      541 atgaccccaa catgcccgcc aagagcgaga cggcctcgcg gaggaatcgt tgtgtcacgg
      601 acctgtacga gccagggtcc acgctgaagg cgatcaccgt ggcagccgcc ctggaagagg
      661 gggtgatccg gcccacttcc ctgttccact gcgccggaag ctatcccatc gggaagcagc
      721 ggatccgttg tgtggtccac gcaccctaca ctaacggcca tggcgcgcaa acgatccgag
      781 gggtgttgcg agagtcgtgc aacatcggca ccgcccagat cgcctcgcgg ctcgggtcac
      841 gcaagctgta tcgatatatc gcagacttcg gcttcattgg cgacacgcgc tccggcctca
      901 tcggtgagcg ccggggctcg ctcaccccgc cgagcacctg ggcgccgatc aagcaagcga
      961 acgtctcctt tggtcaaggc gtcagcgtca ccgcgctgca ggtggccgtc gcctatggag
     1021 cgattgccaa tggcgggaag ctgatgcaac cctacttcat ccgccgcatt gaggatggcg
     1081 agggcaaggt ggtccaggag tatcatccca gtgtggtacg ccaggtcatc tcgaagcaaa
     1141 ccgctgacct actcgccgag tgcctgcagg aagtcgtgga aaagggctcc gggaagcctg
     1201 cccagttgga ggggtacact gtcgcgggca agacaggaag cgcgcagaag gtgatcgagg
     1261 gatggcgcgg atacgccccg gggcgcttcg tcgcatcctt catcgggttt gctcccgttt
     1321 ccgaaccacg catcgtctgt atcgtggtgg tggacgagcc aaaagggagc cactggggcg
     1381 ccacctgcgc cgcgcccgcc ttccgggaga tcgtccaggg ggcgctgtgg tgcatgaagg
     1441 tgccgccgga ccggccggtt gatttgcagc agactgcgct gggtatgcaa cgatgagtgc
     1501 ctcgcccatg acgcgaggcg gcgatccggg agttctggag ggatcggagg cgtctcgcct
     1561 gcgctctgcc ccctggcccc agaggcctct caggaaacgc atcctttgat gggggcgcgc
     1621 ccccctcgcg ttcccggcgc cggatatgat agaatggatg cgagcggttt caggtttctc
     1681 gacgcggtga gggaggcggc gtgcccggct tccggaatcc gcgcttgcgg tctgcttggg
     1741 agatgacgtt gaacctcaag gcgatcattg aaggtctctc gatacagcaa caggcaggtc
     1801 ctgcggtccc gccagccggc ggaacgaatg tgccggtggt ggccgggatc gcctacgact
     1861 ctcggaaggt ggagccgggc gccctcttcg tctgcattcg cggctacaag acggatggcc
     1921 acctctatat cgaggatgcg ctcgcgcgtg gtgccgccgc catcgtggtt gagcaggcat
     1981 cggcaatccc cgcaaacccg ccggtgccgt ttctcctcgt acccaacgcc cggcgggccc
     2041 tggccacgct gagccgcaac gcgcacggcg cacccagccg caaccttcgc ctcgtgggga
     2101 tcacaggcac caacgggaag acaacgacca cctacatggt gcgccacatc ctcttccatg
     2161 ccggccgccc cacgggcgtc attggcaccc tcggcgccct cattggggac cgccccgtgc
     2221 ccctcgaccg caccacgccg gaagctccgg agctggaccg catcctggcc gagatggtcg
     2281 cggctggcgt gcaggattgc gccatggagg tctcctccca cgccatcgcg cttcaccgcg
     2341 tgcgcgagct ggagttcgac atcggcgtct tcaccaacct cacgcaggac cacctcgatt
     2401 tccataagag cctggaggat taccgggccg cgaagatgga aatcgaggtg gtcctgcgtg
     2461 aggttggtga agacgccgat
//
LOCUS       DAOSCV010000681         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27581_length_2478_cov_3.514239, whole genome shotgun sequence.
ACCESSION   DAOSCV010000681 DAOSCV010000000
VERSION     DAOSCV010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27581_length_2478_cov_3.514239"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PLY56_14915"
     CDS             <1..890
                     /locus_tag="PLY56_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ22817.1"
                     /translation="FESFDQAVEVVGRFEQPEYRGTLVRQPTGPHTRQLLLLMEPARA
                     TRSPRPGAVVPFYHPDAMAGYDLEKGEPIRERPVVQFGRHLVQQGYVVVCSEAFPFNT
                     VPHPGTEAGFAWWHAGAKKLLQDNPQWTGMGKLAWDASRALDFLLEQPDVDPERILMI
                     GHSLGGKIAFYAGVLDERVRAVIGSDFGIGWSFTNWDAPWYLGEQIHHPEFPLAHHQL
                     LALLAPRSFLLIGGEADRVESWQYLKAAQEVYRLYGREDAVGHFYHGTGHSPTPESLE
                     IAYRWLAEQFGLPEEAWTL"
     gene            complement(1384..2160)
                     /locus_tag="PLY56_14920"
     CDS             complement(1384..2160)
                     /locus_tag="PLY56_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ22818.1"
                     /translation="MSDSKIALAIAAHPDDVEFMMAGTLALLADAGYECHIFTVANGN
                     CGTAVHSNDEIIRIRAAEGHSAASVIGATYHEGVVNDLEIFYEPETLRRVTAVIREIK
                     PQIVLTQSPQDYMEDHQNACRLAVTACFSRGMRNWVTHPWVEPTMQDVYLYHAQPYGN
                     MGPLREPILPSMVVDITEKIEVKEEMLRRHASQKEWLDVSQGKDAYLVSMRELCAAMG
                     QLAPRKVPYGEGFRQHLHVGLSAKDRDILSEVLGDRVQKL"
BASE COUNT          427 a          812 c          812 g          427 t
ORIGIN      
        1 ccttcgagag cttcgaccag gcggtggagg tagtcggccg gttcgagcag ccggagtatc
       61 ggggaaccct ggtccgccag cccaccggac cgcacacccg gcagctcctc ctgctgatgg
      121 agccggcacg ggccacgcgg tcgccacgcc cgggggccgt ggtgcccttc taccatcccg
      181 acgcgatggc cggctacgac ctggagaagg gggagccgat acgcgagcgc cccgtagtgc
      241 agttcggccg gcatctggtc cagcagggct acgtggttgt ctgctccgag gcgttcccct
      301 tcaacacggt gccccatccc ggcaccgagg cggggtttgc ctggtggcac gcgggcgcca
      361 agaagctcct ccaggataat ccgcagtgga ccggcatggg caagctcgcc tgggatgcct
      421 cgcgcgcgct cgacttcctc ctggagcagc cggacgtaga ccccgagcgc attctgatga
      481 tcgggcactc ccttggggga aagatcgcct tctatgccgg cgtcttggac gaacgcgtcc
      541 gcgccgtgat cggatcggac ttcgggatcg gctggagctt caccaactgg gatgcgccct
      601 ggtacctggg agagcagatc caccacccag agttccccct ggcgcaccac caactcctgg
      661 cgctgctcgc accccgctcc ttccttctca tcggcggcga ggccgaccgc gtggagagct
      721 ggcagtatct gaaggctgca caggaggtat atcggctgta cggccgcgag gatgccgtag
      781 gccacttcta tcacggcacc gggcactcgc cgacgccaga atcgctggag attgcctacc
      841 gctggctcgc ggagcagttc gggttgccgg aagaggcctg gaccctctag atcaggtgac
      901 gtttcggccg cgcggcatca tcccgcggcc ggcatcaccg cctgctcggg cgacttgctg
      961 ccaggaaggc tcaaggcccg ccctcgccgc gctgagctcc cgagataagg gatccgaagc
     1021 ctcgaaggcc gcgcacaacc tggcgcgcgg ggatcaatcc ccgcgctgct tgtgagcgaa
     1081 gccccgccgg gctgggccag accacgtttc cgggcaaccg caaatggcgc ggactgggag
     1141 ggggagcccc gaaggggctt tgccgggaag tagcgcggag cttcgagctc cgcgcgtgcc
     1201 cgcgccctct ccatccgtgt tcgtctcccc aactgcggta ccgaaggtgg cgcgcgggcg
     1261 gcttgctgcc aggaaggctc aaggcccgcc cttgccgcgc gtcatggagg gggcgatagc
     1321 ataatgcggc gccccgtggc gcattgcgcc atggggcgca ctccatccgg caagcactcc
     1381 tcactacagc ttctggacgc gatctcccag cacctcgctc aggatgtcgc ggtccttcgc
     1441 cgagaggcca acgtgcaggt gctggcggaa gccttcgccg tagggcacct tgcgcggggc
     1501 cagctgaccc atcgccgcgc acagctcgcg catcgagacc agataggcgt ccttgccctg
     1561 gctcacatcc agccactcct tctgcgaggc gtggcggcgg agcatctctt ccttcacctc
     1621 gatcttctcg gtgatgtcca cgaccatcga cgggaggatc ggctcgcgca gcgggcccat
     1681 gttgccgtag ggctgggcgt ggtagagata gacgtcctgc atcgtcggct cgacccaggg
     1741 atgggtgacc cagttgcgca taccacggct gaagcacgcg gtcacggcaa gccggcacgc
     1801 attctggtga tcctccatat aatcctgtgg cgattgggtg aggacaatct gtggcttaat
     1861 ctcgcggatc accgcggtca cgcggcggag ggtctccggc tcgtagaaga tctcaaggtc
     1921 gttcaccacg ccctcatggt aggtggcgcc gatcacggag gcggcggaat ggccctcggc
     1981 ggcacgaatg cggatgatct cgtcgttgga atgcaccgcg gtgccgcaat tgccgttggc
     2041 aacggtgaag atgtgacact catagccggc atcggccagc agggccagcg tcccggccat
     2101 cataaactcg acgtcgtccg gatgggcggc tatcgctaga gcaatcttgg aatcggacat
     2161 cagtcaccct ccctagaagc atatatctgt gaagcggttt cgtcgtatcg tccgtgatta
     2221 cctgctacca gatcaagcga gcaaccgggc gcccggaact cccacgctcc ggtgcccggc
     2281 atccccatgc tcgcaccatc gatacctggc aggaatcagg ggcgggcggg cgcgaatagg
     2341 aacgcgaatg cccgtagtgc tccacgcgtg atttgagagc gcgtaatcgg gctcgccact
     2401 ctgtatcgcg agaaaagcta taggcagccg gcctgaaggg agagagtgtc gatgaacatc
     2461 ggtattattg gccttggg
//
LOCUS       DAOSCV010000682         2475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27628_length_2475_cov_4.108678, whole genome shotgun sequence.
ACCESSION   DAOSCV010000682 DAOSCV010000000
VERSION     DAOSCV010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27628_length_2475_cov_4.108678"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PLY56_14925"
     CDS             complement(<1..243)
                     /locus_tag="PLY56_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ22819.1"
                     /translation="MAVRKIVKIDEELCNGCGLCVPSCAEGAIQVIDGKARLVKDEYC
                     DGLGACLGECPQGAIEIIEREAPEFDEAAAMAHVSRM"
     gene            1300..>2475
                     /locus_tag="PLY56_14930"
     CDS             1300..>2475
                     /locus_tag="PLY56_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HOJ22820.1"
                     /translation="MQSDVPHPQGLYDPRYEHDACGVGFLVHLKGKRSHQIVRDGLAA
                     LNNLNHRGASGAEVNTGDGAGVLIQVPHEFLLEQCARLDIALPGRGEYGVGTLFLPRD
                     PSQAAIAKRLFERIVAEEGQRFLGWRPVPVDNRMLGASALSAEPRMEQAFVGWGPKIQ
                     DPDAFERKLYVIRKRHEFAVESSGIPDAEHVYFPSLSCYTLVYKGMLTAEQLALYFPD
                     LRDEALQSAFCMFHSRFSTNTFPNWRLAHPYRMIAHNGEINTLRGNINWMRAREVLLE
                     STLFEPGDLEKLIPITREGLSDTATLDNVVELLVKAGRSPAHAMMMLIPEAWEKHQTM
                     SQEKKDFYAYHACLIEPWDGPASIAFTDGKTIGATLDRNGLRPSRYWVTKDDRVIMAS
                     "
BASE COUNT          470 a          811 c          774 g          420 t
ORIGIN      
        1 catccgggac acatgcgcca tggcggcagc ctcgtcgaac tctggcgcct cgcgctcgat
       61 gatctcgata gctccctggg ggcactcgcc caggcaggcg cccaggccat cgcaatactc
      121 atccttcacc aggcgcgcct tgccatcgat gacctggatc gcgccctcgg cgcaagatgg
      181 cacgcacagc ccgcagccgt tgcatagctc ctcgtcgatc ttcacaatct tgcgaactgc
      241 cattttcccc tccctctcgc gtgtgtgacc ccatagtatc acacacgcga ggtgaagaat
      301 atgactttca tcatatgttc ttgctcaggg aaaatgtcag tagtttgctt agcgaaaatg
      361 tcactaacac acaatggagt ggcgccaggg gatggcactc tcccgagctc atacgcatga
      421 atgcagatag gacatctgcc gcgccctggc ctttgggttg ggcaccgcag gtttggcttg
      481 cgcggcagat ggttgcgact tcgctatcgc gcgctgtcca agcggcatcg tgcgatagca
      541 aagtcgctct tttccatggt agagaccggg tgattacctc aacccggttt tgctgtctct
      601 gttcaaaaga acacactcca tactctactc tacggctcac cgtacggggt aacattttcc
      661 ctgagcaaca acacaacaac ctgggattcc gacataagga atccgaagcc acggaggcag
      721 cgcgcaaccc ggcgcgcggg gagcaatccc cgcgctgctt gcgagcgaag ccctgccggg
      781 ctgggccggg ctacattcgc ggcgcaagcg caacggtgcg ggctgggatg cggagccccg
      841 aaggggcttt gccggaaagc agcgcggagc ttcgagctcc gcgcgtgcgg gcggccctct
      901 ccttatgtcc gaatcccagc acaacaacat ttcctgttga caccaccggg agcgcgtggt
      961 atcataggga gcaaagagga ggatggcggc aaccggttgc ccactaccga cactggcgaa
     1021 aggaagctgc tgcgccaggt cggatcgaca gcgggagcca caggaggccc ttcggggtgg
     1081 gatagcgacg cccctggtcg gaagtggccg gcaaaggagc gccggcaggc ggcggctccc
     1141 ccgggcggca ccctggtggc ggtgcggccc gcgtcttgcg cttctggcgc cagcgagagc
     1201 cgccaccgaa gtaggaaaca attgccgtca ggataccctg ggagagcggc catccggaga
     1261 gtgcccgcgc cgagcgggcg gaagcgagag gttctagaga tgcagagcga tgtaccccat
     1321 ccgcaaggcc tttacgatcc gcggtacgag cacgacgcgt gtggcgtcgg cttcctggtg
     1381 catctcaagg gcaaacgctc gcatcagatt gtccgcgatg gcctcgccgc actgaacaac
     1441 ctgaaccacc gcggtgcgag cggcgcggag gtgaacacgg gcgacggcgc cggcgtgctc
     1501 atccaggtgc ctcacgagtt tctcctggag cagtgcgccc ggctggacat cgccttgccc
     1561 ggccggggcg aatacggcgt tggtacgctc ttcctgccgc gcgatccgag ccaggcggct
     1621 atcgcgaagc gcctcttcga gcgcattgtc gccgaggaag gccagcgctt cctcgggtgg
     1681 cgcccggtgc cggtcgataa ccgcatgctc ggcgccagtg cgctctcagc cgagccgcgc
     1741 atggagcagg cgttcgttgg ctgggggccg aagatccagg accccgacgc cttcgagcgg
     1801 aagctctacg tcatccgcaa gcggcacgag ttcgccgtgg agagcagtgg catccccgat
     1861 gccgagcacg tttacttccc cagtctctcc tgctacacgc tcgtctacaa ggggatgctg
     1921 acggcagaac agctcgcact ctacttcccg gacctgcgtg acgaggccct gcagagcgcg
     1981 ttctgcatgt tccactcgcg tttcagcacc aacaccttcc cgaactggcg gctggcgcac
     2041 ccctaccgga tgatcgcgca caacggcgaa atcaacaccc tgcgcgggaa catcaactgg
     2101 atgcgtgccc gcgaggtgct cctggagtcc accctcttcg agcccggcga cctggagaag
     2161 ctcatcccga tcacccgtga gggcctgagc gataccgcga cgctcgacaa cgtggtggag
     2221 ctgctggtca aggcggggcg cagtccggcg cacgcgatga tgatgctgat ccccgaggcg
     2281 tgggagaagc accagacgat gtcgcaggag aagaaggact tctacgccta ccacgcctgc
     2341 ctcatcgagc cgtgggacgg ccccgcgtcc atcgccttca ccgacggcaa gacgatcggc
     2401 gccaccctcg accgcaacgg cctgcgcccc tcgcgctact gggtgacgaa ggacgaccgg
     2461 gtgatcatgg cctcc
//
LOCUS       DAOSCV010000683         2472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27672_length_2472_cov_3.121638, whole genome shotgun sequence.
ACCESSION   DAOSCV010000683 DAOSCV010000000
VERSION     DAOSCV010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27672_length_2472_cov_3.121638"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLY56_14935"
     CDS             <1..760
                     /locus_tag="PLY56_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ22821.1"
                     /translation="ADVVTLALAYDVDALQKAGLIRQGWQNRLPHRSAPYTSTIVFLV
                     RRGNPKRIRDWSDLIRPDVKVITPNPKTSGGARWNYLAAWGFAMRQSGGNAREFVTRL
                     FKNVPVLDTGARGSTTTFVERGIGDVLLAWENEALMVMNKLGKGQFTLVTPPTSILAE
                     PPVAVVDRVVDRRGTRRVAEAYLRYLYSPEGQEIAARHYYRPRLAAVAKKYSSQFART
                     RLYTIDQIAGGWTKAHAKHFADGGIFDQIYVPGR"
     gene            1015..1848
                     /gene="cysT"
                     /locus_tag="PLY56_14940"
     CDS             1015..1848
                     /gene="cysT"
                     /locus_tag="PLY56_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOJ22822.1"
                     /translation="MPSRRLRRGVLPGFGPALGLSLFYLCLLVLLPLSTIFLRSSTMG
                     WQAFWEAVTAPRVIASYRLSLLASLGAALINAFFGFLVAWVLVRYRFPGRDIVDALVD
                     LPFALPTAVAGIALTAIYGPEGWLGRPLAHLGVQAVFNPLGILIALTFIGLPFAVRTV
                     QPVLEGLDKAEEETAASLGASRWQTFRRVILPPVLPAVLTGFALAFARALGEYGSVVF
                     ISGNMPMRTEVAPLLIMTKLEQFDTEGATAIAVVLLGLSFALLLAINLLQARQQRLAG
                     Q"
     gene            1865..>2472
                     /locus_tag="PLY56_14945"
     CDS             1865..>2472
                     /locus_tag="PLY56_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ22823.1"
                     /translation="MNATKTKTLPDGVTEDPPWIRRLLIATALLFLVLFLFVPLAAIF
                     KEAFSNGLAEYWWALSDPDALAAIRLTLLATVIAVPLNLIFGIAASWAIANFEFRGKS
                     VLVSLIDLPLAVSPVIAGMVFVLLFGTNGLLGAWLEAHHLKIIFATPGIVLATAFVTF
                     PFVARELIPVMQARGSEEEEAALVLGASGWQMFWRVTSPTSAG"
BASE COUNT          372 a          829 c          800 g          471 t
ORIGIN      
        1 ggccgacgtc gtcaccctgg ccctggcgta tgacgtggat gcgctccaga aggctggcct
       61 gattcgccag ggatggcaga accgcctccc ccaccgaagc gcgccctaca cgtcgacgat
      121 cgtcttctta gtccgccgcg gcaatccgaa gcggattcgc gattggagcg atctgatccg
      181 ccctgatgtc aaagtgatca cgccgaatcc gaagacctct ggcggcgcgc gctggaacta
      241 cctggccgcc tgggggttcg cgatgcggca atcgggcggg aacgcgcgag agttcgtcac
      301 ccggctcttc aagaacgtgc cggtgctcga cacgggcgcg cgcggctcga ccaccacgtt
      361 tgtcgagcgc ggcatcggcg acgtgctcct ggcctgggag aacgaggccc tgatggtgat
      421 gaacaagctc ggcaaaggcc agttcaccct ggtcaccccg cccacgagta tcctggccga
      481 gccgccggtg gccgtggtcg accgcgtggt cgaccgccgg ggcacgcgcc gcgttgccga
      541 ggcatatctg cgctatctct acagccccga gggccaggag atcgcggcgc gccactacta
      601 ccgtccgcgc ctggccgcgg tggcgaagaa gtactcgagc cagttcgcgc gcacccggct
      661 ctacaccatc gaccagatcg ccggcgggtg gactaaagcg cacgcgaagc acttcgcgga
      721 tggcgggatc ttcgatcaga tttacgtgcc cggacggtga tgccacgggg cttttgcggc
      781 catgggtcgg cgccgccacc cgcggtgcag ccccggacgc cactaacccg cattattcat
      841 cagaataatg aaaagaagtc ttactgccag gcgtggcacg ggttcgtggg cattgccccc
      901 gaaaacaccc tgcctccggg cgcttgtagc ggcaacggtg tgtcaccaga caagagactt
      961 ctctttttga agtattctgg tgaatacttc gggctaaacg aaaggaggtt cgcgttgccc
     1021 agccggcgcc ttcgccgagg cgttctcccg gggttcggac ccgccttggg cctcagcctt
     1081 ttctacctgt gcctgctcgt gctgctcccg ctctccacaa tcttcctgcg gagctccacg
     1141 atgggctggc aggcattctg ggaagcggtc accgcgcccc gggtgatcgc ctcataccgg
     1201 ctcagcctgc tcgcctcgct cggcgcggcg ctgatcaacg ccttcttcgg gttcctggtc
     1261 gcctgggtgc tggtgcgcta ccgcttccct ggccgggata tcgtggatgc cctggtcgac
     1321 cttcccttcg cgctcccaac cgccgtcgcg ggaatcgcgc tcaccgccat ctacggcccg
     1381 gaaggatggc tgggccgacc tctcgcccat ctgggcgtgc aggcggtgtt caacccgctg
     1441 gggatactca tcgccctgac gttcattggg ctgccgttcg cggtgcgcac ggtgcagccg
     1501 gtcctggagg ggttggataa ggccgaagag gagacggcgg cgagcctggg cgccagtcgc
     1561 tggcagacgt tccgccgggt gattctcccg ccggtgctcc cggcggtgct caccgggttt
     1621 gcgctggcgt tcgctcgcgc tttgggagaa tatggctcgg tcgtcttcat ctcgggcaac
     1681 atgccgatgc gcaccgaggt ggcgccgctc ctcatcatga ccaagctgga gcagttcgat
     1741 accgagggcg ccaccgccat cgccgtggtg ctcctggggc tctctttcgc gctgttgctg
     1801 gcgatcaacc tgctacaggc gcggcagcaa cgcctcgccg gccagtgatc tcgggagggg
     1861 acgcatgaac gcaaccaaga ccaagacact tcccgacggc gtgacggaag atcccccgtg
     1921 gatccgccgc ctgctgatcg ccacggccct gctcttcctg gtgctctttc tcttcgtgcc
     1981 cctggccgcc atcttcaaag aagcgttctc gaacggcctg gccgagtact ggtgggccct
     2041 cagcgacccg gacgcgctgg ccgctatccg gctcacactg ctcgccacgg tcatcgccgt
     2101 tccgctgaac ctgatcttcg ggatcgcggc ctcttgggcg attgccaact tcgagtttcg
     2161 ggggaagagc gtgctcgtca gcctgattga cctgccgctc gccgtttcgc cggtgatcgc
     2221 gggcatggtg ttcgtgctcc tctttgggac gaacggtctg ctcggggcct ggctggaagc
     2281 gcatcacctg aagatcatct tcgcgacgcc ggggatcgta ctggcgaccg ccttcgtcac
     2341 cttccccttc gtggcgcgcg agctgatccc ggtgatgcag gcccgcggga gcgaagagga
     2401 ggaggccgcg ctggtgttgg gggccagcgg gtggcagatg ttctggcgcg tgacctcccc
     2461 aacatccgct gg
//
LOCUS       DAOSCV010000684         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27749_length_2466_cov_2.995023, whole genome shotgun sequence.
ACCESSION   DAOSCV010000684 DAOSCV010000000
VERSION     DAOSCV010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27749_length_2466_cov_2.995023"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLY56_14950"
     CDS             complement(<1..918)
                     /locus_tag="PLY56_14950"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ22824.1"
                     /translation="MQALVKTQKGVGFLELREVPVPKPGPGEVLIAVKAAGICATDVH
                     VKHDRFPYWPPVVLGHEFSGEIVELGPEVQRFRVGDRVVGEPHTQACGHCYLCRTGNI
                     QICPTKRSPGWGIDGAFTSFLKMPERLLHRIPDDMSFDVAAVVEPTANTVHDVIERAK
                     VEAGDFVVVLGPGPIGLLAGLTARAGGARHVVIIGAPSDEAVRLKKARELGFDTVINL
                     AETNPVEVVQDLTDGIGADLVIDCSGAPPAIASTVDLVRKKGRICAIGLTGRESVSFP
                     WDKAASKVVDIAFCMSTSYTSWDRTINLIA"
     gene            complement(964..2220)
                     /locus_tag="PLY56_14955"
     CDS             complement(964..2220)
                     /locus_tag="PLY56_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ22825.1"
                     /translation="MSQLSIEQIRAAVEAGRADAEQFLCELIRFPSTPGNETEAMACA
                     ARRFGELAEVERVPLSNALREDEDYADPVPNLDYEGRFNLRLRLPGKGGGRSLLFNTH
                     LDVVPPSQGQESPWDPRVDAGVVRGRGACDAKGQAAALYLALWAIRRLGLKLDGDLLG
                     HLVVEEEVGGNGTLAMVRRGEKADACVVMEPTDLRIFSSVRGAVWFRVTCTGRPGHSG
                     RAGDTVSALKMAIRVVEILEGYHARLLASSRGFPLFDPFPNPMPITFGKLQAGDWPAT
                     APSRAVVEGVLGLLPNKTRYQVMEEMRQAILDEGDEWLREHFELEFMYRHDAHVLDVA
                     HPLVTQLQACCREVGAAGEVTAMTASCDSWFYNNQLGIPTVVFGPGSLGFAHSNEEQI
                     RMDEIVDAAAVLVRLAESWCVRERGA"
BASE COUNT          410 a          896 c          768 g          392 t
ORIGIN      
        1 agcgatgagg ttgatcgtcc ggtcccaact cgtatagctc gtggacatgc agaaggcgat
       61 atccaccacc ttggatgccg ccttgtccca ggggaaggag acgctctccc ttcccgtgag
      121 cccgatggcg cagatgcggc ccttcttgcg caccaggtcc accgtcgagg cgatggccgg
      181 cggcgccccg ctgcaatcga tcaccaggtc ggcgccgatg ccatccgtca ggtcctgaac
      241 aacctccact gggttcgtct cggccaggtt gatcacggta tcgaagccaa gctcgcgcgc
      301 tttcttcagg cgaaccgcct cgtcggaagg cgcgccgatg atcaccacgt ggcgggcacc
      361 tccggcgcgc gcggtcaacc cggccaggag gccaatcggt ccggggccga ggacgaccac
      421 gaagtcgccc gcttccacct tcgcgcgctc gatgacgtca tgcacggtgt tcgccgtcgg
      481 ctcgaccacg gcggcgacgt cgaagctcat gtcgtcggga atgcggtgga gcagtcgctc
      541 cggcatcttc agaaagctgg tgaaggcgcc atcgatcccc cagccgggcg agcgcttcgt
      601 cgggcagatc tggatgttcc cggtccggca aaggtagcag tgcccgcacg cctgggtgtg
      661 cggctcgccg acgacgcggt cgcccacgcg gaagcgctgc acctcggggc ccagctccac
      721 gatctcgccg gagaactcat gcccgaggac aaccggtggc cagtagggga agcggtcgtg
      781 cttcacatgc acatccgtgg cgcagatgcc agccgccttg acggcgatga gcacctctcc
      841 cggccccggc ttcggcacgg gaacctcgcg cagttccaga aagccaacgc ccttctgtgt
      901 ctttactaac gcttgcatgc tgatcattct ccctggagcg ctggaggcag gccgccccct
      961 gccctacgcc cccctctctc gcacacacca actctccgcg aggcgcacga gcacggccgc
     1021 ggcgtctaca atctcatcca tccggatctg ctcttcgttc gagtgcgcga agcccaggct
     1081 gcccgggcca aagaccaccg tggggatgcc cagctggtta ttgtagaacc aggagtcgca
     1141 cgaggcggtc atcgccgtca cctcgcccgc ggcgcccact tcgcggcaac acgcctgcag
     1201 ctgcgtgacc agtgggtgcg ccacatcgag gacgtgcgca tcgtgccggt acatgaactc
     1261 cagctcgaag tgctcgcgca gccactcgtc gccctcatcg aggatcgcct ggcgcatctc
     1321 ctccatcacc tggtagcgcg tcttgttcgg cagcagcccg aggacaccct cgaccacggc
     1381 ccggctcggc gccgtcgccg gccaatcgcc cgcctgcagc ttcccgaacg tgatcggcat
     1441 cgggtttggg aaggggtcga agagggggaa gccgcgagaa ctggcgagca ggcgcgcgtg
     1501 gtagccttcc aggatctcca ccacgcggat cgccatcttc agggcgctca ccgtatcgcc
     1561 cgcgcgcccg ctgtgccccg gccggccagt gcatgtcacc cggaaccaca ccgcgcctct
     1621 caccgaggag aagatccgca ggtcggtcgg ctccatcacg acgcacgcgt ccgccttctc
     1681 accgcgccgg accatcgcga gcgtcccgtt gccgcccact tcctcctcca ccaccaggtg
     1741 gccaaggaga tcgccgtcca gcttcaggcc cagccggcgg atcgcccaga gcgccagata
     1801 gagcgccgcg gcctgcccct tggcgtcgca ggcgccgcgc ccgcggacca cccccgcgtc
     1861 cacgcgggga tcccatgggc tctcctggcc ttgcgagggg ggaaccacat cgaggtgggt
     1921 gttgaagagg agcgagcgcc cccctccctt ccccggcagg cgcaggcgga ggttgaaccg
     1981 cccctcatag tccagattgg gaacgggatc ggcgtaatcc tcatcctcgc gcagtgcgtt
     2041 ggagaggggc acgcgctcta cctctgccag ctcgccaaag cgacgcgcgg cacaggccat
     2101 cgcctctgtc tcgttgcccg gcgtcgaggg aaagcggatc aattcgcaga gaaactgctc
     2161 cgcgtccgcc cggccggcct ctaccgccgc gcggatctgc tcgattgaga gttggctcac
     2221 agcggccttc ctaactccat ctccgatggc gaaccggacc ccacgccacg gcgggcctca
     2281 cgggcgcacc cgccgtcacg acaccgccag cggtgtcgcg acatcgtgct gtcggggatc
     2341 acgtatcgcc ggcatgaggt ccaagcgcca gaaaagcggc gctccctcga agagtgtgtg
     2401 taggaggcgt cggcggcccg agcatgtaag ccggtcttca cgctcttttc agacacgccg
     2461 ctacag
//
LOCUS       DAOSCV010000685         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27801_length_2463_cov_2.618771, whole genome shotgun sequence.
ACCESSION   DAOSCV010000685 DAOSCV010000000
VERSION     DAOSCV010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27801_length_2463_cov_2.618771"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /gene="eno"
                     /locus_tag="PLY56_14960"
     CDS             <1..473
                     /gene="eno"
                     /locus_tag="PLY56_14960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879281.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ22826.1"
                     /translation="RLVDTYPIISIEDGMAEDDWDGWKMLTDAIGNRVQLVGDDLFVT
                     NTERLERGIREGVANSILIKVNQIGSLTETLQAIEMAKRAGYTAVISHRSGETEDATI
                     ADIAVATNAGQIKTGAPSRTDRVAKYNQLLRIEEQLDDLAQYRGIEVFYNLRDR"
     gene            692..2101
                     /gene="pyk"
                     /locus_tag="PLY56_14965"
     CDS             692..2101
                     /gene="pyk"
                     /locus_tag="PLY56_14965"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ22827.1"
                     /translation="MRRTKIVCTIGPASSSPEILERLIREGMDVARLNFSHGTHEEHA
                     QNIRTIREIAKRLGKPVAILQDLSGPKLRIGEMAGEVTLVPGQPFTLTTRDIVGNEQC
                     ASIPIEDLPASLSPGNRLFLNDGIIELTVAETTETEIRCVVRSGGRLSSHKGLNVPDV
                     SINIDPITPKDLEDLDFGIDHGVDWVAASFIRRAEDLARLRERMAARGVQIPILAKIE
                     KHEAVKNLDAILNAADGAMVARGDLGIEVSIDEVPIIQKRIIARCNALAKPVITATEM
                     LDSMHDRPRPTRAEVTDVANAIFDGTDCVMLSRETATGRFPVETVQMMHRIAVRTEES
                     ERYQRALHTREDTVAQNVTDAIGEAIRTMAQDLKVAAVVPATASGYTARMVAKYRPIC
                     PIIAVSHLPETVQRLALTWGVQPVLSSPVGTTDEMLREAVTVSLATGLVKRGDLILIS
                     AGVPIGTPGHTNLIKVERV"
BASE COUNT          469 a          827 c          791 g          376 t
ORIGIN      
        1 cccgcctggt ggacacctac ccgatcatca gcatcgagga cggcatggcc gaggatgact
       61 gggacggctg gaagatgctg accgatgcca ttggcaaccg cgtccagctc gtgggcgatg
      121 acctcttcgt caccaacacc gagcgcctgg agcgcggcat ccgcgagggg gtcgccaact
      181 cgatcctgat caaggtcaac cagatcggct cgctcaccga gacgctgcag gcaatcgaga
      241 tggccaagcg cgcaggctac accgccgtca tctcgcaccg ctctggcgag acggaagacg
      301 ccaccatcgc cgatatcgcc gtcgccacca acgccggcca gatcaagacg ggcgctccgt
      361 cgcgcaccga ccgcgtcgcc aagtacaacc agctgctgcg catcgaggag cagctggacg
      421 acctggccca gtaccggggc atcgaggtct tctacaacct gcgcgaccgc taatctataa
      481 caatcgcggg gtggcccgag tccggggtag gaccccaggc tccggccatc ccgttctaca
      541 agcagggatc cgcactctat ccggttgggg agcggcaggg ccaggcggga taacgcatcg
      601 atcgcgcccg acccacgtgt ggaccttgcc aggagagggc gcggtattcc tacacgcccg
      661 cgttcccatc ccggctccga gggggagaag catgcgccgt acgaaaatag tctgcaccat
      721 cggcccggcc tcaagcagcc cggagattct ggagcgcctg atccgggaag ggatggatgt
      781 cgcccgcctc aacttctcgc acggcacgca tgaggagcac gcccagaaca ttcggacgat
      841 ccgagagatc gccaagcgcc tcgggaagcc ggtcgccatt ctccaggacc tctccgggcc
      901 gaagctgcgc atcggcgaga tggccggcga agtcaccctc gtgccgggcc agccgttcac
      961 gctcaccaca cgcgatatcg ttggcaacga gcagtgcgcg tcgatcccca tcgaggacct
     1021 tccggcgagc ctatcgcccg gcaaccgcct cttcctgaac gacggcatca tcgagctcac
     1081 cgtggccgag accacggaga cggagatacg ctgcgtcgtt cgctcgggcg gtcggttgag
     1141 cagccacaag gggctcaacg tgccggatgt ctctatcaac attgatccca tcacgcccaa
     1201 ggatctggag gacctcgatt ttgggatcga ccacggcgtg gactgggtgg cggcctcgtt
     1261 catccgcagg gcagaggacc tggcgcggct gcgtgagcga atggcggcgc gaggcgtgca
     1321 gatccccatc ctggccaaga tcgagaagca cgaggcggtg aagaatctcg acgcgatcct
     1381 gaatgccgcc gatggcgcta tggtcgcccg tggcgacttg gggatcgaag tctccatcga
     1441 cgaagtgccg atcatccaga agcggatcat tgcccggtgc aacgccctgg ccaagcccgt
     1501 catcaccgcg acggagatgc tcgactcgat gcatgaccgc ccgcgcccca cgcgcgcgga
     1561 ggtcaccgat gtcgccaacg ccatcttcga cggcaccgat tgcgtgatgc tctcccgaga
     1621 gactgccacc gggcgctttc ctgtcgagac ggtgcagatg atgcatcgga tcgccgtgcg
     1681 caccgaggaa tcggagcgct accagcgtgc cctccacacc cgtgaggaca ccgtggcaca
     1741 gaatgtgacc gacgccattg gtgaggcgat ccgtaccatg gcgcaggacc tgaaggtggc
     1801 cgccgtcgtg ccggcgaccg cttccggcta caccgcgcgc atggtggcga aataccggcc
     1861 catctgcccc atcatcgccg tttcacacct ccccgagacg gtgcaacgcc tcgcgctcac
     1921 gtggggagtc cagcccgtgc tctccagtcc ggtgggcacc acggatgaaa tgctgcggga
     1981 agcggtgacc gtctcgctgg cgacgggcct ggtgaagcgt ggagacctga tcctcatctc
     2041 cgccggggta ccgatcggca ccccagggca caccaacctg atcaaagtgg aaagggtgta
     2101 ggcgcagctc aaggttgtag gtaacgggcc gcggcagcga gagcggttca tgccgctcgg
     2161 cctgcccggc aggggaacgc gcgcggtccg cggcgcgggc ccgctttctc tagcgaatgg
     2221 ggcaagcagg tgaaaaagcc tccaggtaaa cagcacgagc cactgctaat ccggcgaaga
     2281 gcgcgccgcg ccgggaaccc tcggatgaga cggttgtggc ggctgaccaa gcgcctgttc
     2341 cagtgcctcg ccgccgcggc ggcactggtc tggctcacct gcgccgcgtt gcgcccttac
     2401 ctgctagacc gcgaacaggt ggcggcgatt cgccgactct ccgctgaagt cgcggtggtg
     2461 gaa
//
LOCUS       DAOSCV010000686         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27839_length_2461_cov_2.798421, whole genome shotgun sequence.
ACCESSION   DAOSCV010000686 DAOSCV010000000
VERSION     DAOSCV010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27839_length_2461_cov_2.798421"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLY56_14970"
     CDS             complement(<1..223)
                     /locus_tag="PLY56_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22828.1"
                     /translation="MWTGMVQPYVRNTGVTQCPSNPTPRNAARGCGGYGYNLSTLANG
                     TAVGCGNRQLADIEKPAGLLMICESGDGTA"
     gene            complement(311..478)
                     /locus_tag="PLY56_14975"
     CDS             complement(311..478)
                     /locus_tag="PLY56_14975"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22829.1"
                     /translation="MRSRITAGPAGRHGFTLIELLVVIAIIAILAAILFPVFARAREN
                     ARKSTCQSNIK"
     gene            633..>2461
                     /locus_tag="PLY56_14980"
     CDS             633..>2461
                     /locus_tag="PLY56_14980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ22830.1"
                     /translation="MKSATQNDPVARAYATREHPARPGGKTLKASYPLIALLALSAAA
                     PGAAEPGPANLLLNPQSRFHAFDNSREGKADSSTSGAVPCWDQDAYGDVQVTRSPRVT
                     AFRPRFPVENVVAIRPGKRLSQFALLVEMGLDHGDRVSFSLQGYQTTPGALRANIVPM
                     RLDSAAGSWSPAEFGQEDKRRFPRHARGELVRGPGGTAVSGGAGHFELKVENVELAGA
                     FTESGDQSTDEPNTIGLLVELVSLAEEGDVFVYAPCLCRGATAQSRLPEARPLPTYYR
                     GIPRTMQKLWRGEPLHIIAMGSSIDRGSANPPMYLYDEDPASPSFKQPLTGREFDGTR
                     IGRPEWNDYIGWWQHHFMYTGRLRQALMRKFNYPMEKLLLNTMACDGSSISESHSGLA
                     DYATLAFPPDPNRNGHRTGKTWQELYPQLFTRPEGPRPDLVIFGSGANEKVDGADEVA
                     AFEGAIRWFQRHYPDTEFLFCMFQNRERYTPNTGHLMELALRYQIPYIDFGRLFHLAT
                     RHCNSYALVPKDGHPQAAGHYLWFKQLERAFDAADPIEPGIAQLHLPERLSPYTIGWE
                     GDMTTYTAPHPRIRQGTAFIFDDTVVNLWASAGDIVEIRLDGAP"
BASE COUNT          443 a          842 c          753 g          423 t
ORIGIN      
        1 cagccgtacc atcaccgctc tcgcagatca tcaacaggcc cgcgggcttc tcgatatcgg
       61 cgagctggcg gttcccgcag cccacggccg tcccgttcgc cagggtcgac agattgtagc
      121 catagccccc gcatccacgg gccgcgttcc gaggagtggg gtttgacggg cactgggtca
      181 cgccggtgtt cctcacgtac ggctgcacca tgccggtcca catgctggga tctgctccgg
      241 cgggctggat gcagtgccgg ggcagccgct cgtcatagtc ctgggcatac tgcagaactc
      301 cgagcgccag ctacttgatg ttcgattggc acgtgctctt acgcgcgttc tccctggccc
      361 gggcaaagac agggaagagg atcgccgcga gaatggcgat tatcgctata actaccagga
      421 gctcaatcag ggtaaacccg tgtctgccgg ccggcccggc agtgattcgt gaacgcattt
      481 gccactcctc tcccttcagt gtataggcgg aagcgagagg ctcgcgtgga gtgccgggga
      541 gagtatacca ccatccacga gccgtgtcaa cccataccgc gcggggttct tgctatccac
      601 agacgagtgc ggccaggaag attggacagg aattgaagag cgccacccag aacgatccgg
      661 tggcgcgcgc ctatgccaca cgcgaacacc cggcgcgccc tggagggaag accttgaaag
      721 cctcttaccc gctcatcgct ttgctcgctc tttccgcggc cgcgcccggc gctgccgagc
      781 cggggccagc caatctgctg cttaaccccc aatcccgctt ccacgccttc gataactcgc
      841 gcgaaggcaa agcggatagc tccacctcgg gggcggtacc ctgctgggac caggatgcct
      901 acggcgatgt tcaggtcacg cgctctcccc gggtgacggc gttccggcca cgcttccctg
      961 tggagaacgt cgtcgcgatt cgccccggaa agcgcctatc gcagttcgct ctcctggtcg
     1021 agatgggcct ggaccacggc gaccgggtga gcttctctct gcaaggctat cagacaactc
     1081 cgggggcgct gcgcgccaac atcgtgccga tgcgcctcga tagcgccgcg ggcagttgga
     1141 gtcccgccga gttcggccag gaggataagc gccgcttccc caggcacgcg cgcggcgagc
     1201 tagtccgcgg gccgggcggc acggccgtct ccggtggcgc cggccacttc gagctgaagg
     1261 tcgagaatgt ggagctggcc ggtgcgttca ccgagtcggg cgaccaatcc acggatgaac
     1321 cgaacaccat cggcctcctg gtggagctgg tgagcctggc ggaagagggc gacgtcttcg
     1381 tgtacgcccc ttgcctctgc cggggtgcca cggcgcaatc gcgccttccc gaggcacgcc
     1441 cgctgccgac ctactatcgc ggcattccac gaacgatgca gaagctgtgg cggggcgagc
     1501 cgctgcacat catcgccatg ggatcgagca tcgaccgagg cagcgccaac ccgccgatgt
     1561 acctctatga tgaggatccc gcgtcgccca gtttcaagca gccgctcacc gggcgcgagt
     1621 ttgatggcac gcgcattggc cgacccgagt ggaacgatta catcgggtgg tggcaacacc
     1681 acttcatgta caccggaagg ctacgccagg cgctgatgcg caagttcaac tatccgatgg
     1741 agaagctgct cctcaacacc atggcgtgcg atggctcctc cattagcgag tcgcactccg
     1801 gcctcgccga ctacgcgacg ctggccttcc cgcccgatcc caaccgcaac ggccaccgca
     1861 ctgggaagac atggcaggag ctctaccccc agctcttcac gcgcccggag gggccgcgcc
     1921 cggatctcgt catcttcggc agcggcgcga acgaaaaggt ggatggcgcc gacgaagtgg
     1981 ccgcgtttga aggcgcaatc cgctggttcc agcggcacta cccggatacc gagttcctct
     2041 tctgcatgtt ccagaaccgg gaacgctaca ccccgaacac cggtcacttg atggagctgg
     2101 ctctccggta tcagatcccc tacatcgact tcgggcgcct ctttcacctc gccacgcgcc
     2161 actgcaacag ctatgcgttg gtcccgaaag atggacatcc ccaggcggcc ggccactacc
     2221 tctggttcaa gcagctcgag cgtgccttcg acgccgcaga cccgatcgag cctgggattg
     2281 cccagctcca cctcccggag cgcctgagtc cctacaccat cgggtgggaa ggcgacatga
     2341 ccacctacac cgcgccccac ccgcggatcc gccagggcac agccttcatc tttgacgata
     2401 cggtggtcaa tctttgggcg agcgcaggtg atattgtcga gatccgcctg gacggcgcgc
     2461 c
//
LOCUS       DAOSCV010000687         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_27887_length_2458_cov_3.484395, whole genome shotgun sequence.
ACCESSION   DAOSCV010000687 DAOSCV010000000
VERSION     DAOSCV010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27887_length_2458_cov_3.484395"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLY56_14985"
     CDS             complement(<1..712)
                     /locus_tag="PLY56_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22831.1"
                     /translation="MKRYWSAILAATLLPACAGRAGAVSFLPIPDNAANEFSWGAGLA
                     PGGEVSQPPSRFRVGLFAAHWKVPEVSISHYTGQAGDAVRFEATTMPVVEVDYRLGKR
                     LRLGAWYNPADTRVWLKGPTADLLKTSPQPVIGSTHLDRTSLNMWNVNLAYQLPNETA
                     LALGVTRYQGTLAMRVPNWRGSDDLAFTAELGATELSMWGYRSLRIWEYTQRPAYLTG
                     GLGLCHRISHDSGSPGSKT"
     gene            complement(941..1735)
                     /locus_tag="PLY56_14990"
     CDS             complement(941..1735)
                     /locus_tag="PLY56_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22832.1"
                     /translation="MKHLGTIAAVAGLLLQAGAAHAISFAPPRPIVPTAVEAPSGERT
                     ASLTLGLAAASWDQPDQAIRDSAGALLGTIEFGTQVRPLITAEYRINDTWSLGCWYNF
                     VSWDTVLHPAAVPGSVVAADASVDGGIYELHVTRDFPVKSRWDLSAQVGFQHTKYDGT
                     SRVTTVGQGTRTQKFEYDSQQAVAWGLGTYKIPGARENAPSFGLMGGLGLSQGLSGEG
                     SGTQAHLLLGVSAAFTPTISATASMWLVDLTDDSLARFTAGVTGRF"
     gene            1737..1889
                     /locus_tag="PLY56_14995"
     CDS             1737..1889
                     /locus_tag="PLY56_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22833.1"
                     /translation="MSVLLSQAVVTVARAPRELGSLDPTDPPVAEGPARLSGSAGGWV
                     ATIGVW"
BASE COUNT          386 a          828 c          824 g          420 t
ORIGIN      
        1 tcgtcttcga gccaggagag ccggaatcat gcgagatgcg gtggcagagc ccgaggccgc
       61 ccgtgaggta ggccggcctc tgcgtgtact cccagatgcg caggctgcgg tagccccaca
      121 tactcagctc cgtcgccccc agctccgcgg tgaaagccag gtcgtctgac ccgcgccagt
      181 tcggcacccg catcgccagc gtgccctggt aacgcgtcac cccaagggca agcgccgtct
      241 cgttcggcag ctggtaggcc agattgacgt tccacatgtt caggctggtg cgatccagat
      301 gcgtcgagcc gatgaccggc tgcgggctgg tcttcaggag gtcggccgtc gggcccttca
      361 gccagacgcg cgtatccgcc gggttgtacc aggctcccag gcgcaggcgc ttgccgaggc
      421 ggtaatccac ttcgacgacg ggcatcgtgg tagcctcgaa gcgaacggca tcgccggcct
      481 gcccggtgta gtgggagatg ctcacctcgg gtaccttcca atgggcggca aagaggccca
      541 cgcggaagcg ggagggcggc tgtgagacct cgccgccggg cgccagtccg gcgccccacg
      601 agaactcgtt ggccgcgttg tctggaatcg gcaggaagct aacggcgccc gcgcgcccgg
      661 cgcaggcggg aagcagcgta gcggcgagga tagcgctcca gtatctcttc attggtcaga
      721 ccgtttcctc tacttgctgg gcggcgacac cgccgcaatg ctctcttgcg gcgccgccgc
      781 cggtgggtaa ccgaaccgtc gtgtggcatc gatccgggcg caccccctga tagggaaggg
      841 gccgcctggc gcactcttct attccccgtc cgggtctcag gcacctttcc gattccaggg
      901 tgggatcctc aacctgctct gccacgttca ggctcgcctg tcagaagcgc cccgtcacgc
      961 cggccgtgaa gcgcgccagg gagtcatccg tgagatcgac cagccacatc gatgcggtcg
     1021 cgctaatcgt tggggtgaac gcagccgaga cccccaggag gaggtgggct tgcgtgccgc
     1081 tgccctcgcc ggagaggccc tgagagaggc cgagcccacc catcagccca aagctgggcg
     1141 cgttttcgcg cgcgcccggg atcttatagg ttcccaggcc ccacgccacc gcctgctggc
     1201 tatcatactc gaacttctgc gttcgggtgc cttggccaac cgttgtcacc ctgctcgttc
     1261 catcatactt cgtgtgctgg aatcccactt gcgccgagag atcccaccgg ctcttgacag
     1321 ggaagtcgcg cgtgacgtgc agctcataga tgccgccatc gacgctggca tcggcggcca
     1381 ccacgctgcc tggcacggcc gcagggtgga gcaccgtgtc ccaggagacg aagttatacc
     1441 agcaacccag ggaccaggta tcgttgatgc ggtattccgc cgtgatcaac ggtctcacct
     1501 gcgtgccaaa ctcaatggtg ccgagcagag caccggcgct atcgcgaatt gcctggtccg
     1561 gctgatccca ggacgcggcg gccaggccta gcgtcaggct cgccgtcctc tcgccgctcg
     1621 gggcttcgac cgccgtcggg acaatcgggc ggggaggcgc aaaggagatg gcatgggccg
     1681 ctcccgcctg caacagcagg cccgcgaccg ctgctatcgt gccaagatgc ttcatcgtga
     1741 gtgtactcct ctcgcaggct gtcgtcaccg tggctcgggc accgcgcgag ctcgggagcc
     1801 tggatccaac cgatccgccc gtcgcagagg ggcccgcgag gctctccggc agcgcgggcg
     1861 gctgggtggc caccattggc gtctggtgac tgtggcaagt gtgccgcggc aaggccggcc
     1921 cttggacccg agacgcgcga tggctcgcgc cttccaggat caactgagcg gtggctatca
     1981 cgcggcggtg ttgcgcgctt ccgagttgca ggtgcgtact ggaatcgccc ctcgcgcggc
     2041 acctgctcgc atccctggcg tgtcagagac tcattatcgc tctggggctc gggttccttc
     2101 cccctggcca agcggtgggt actgggaatg atagaggccg gtccgcttgg gctggcgcgg
     2161 cgcgtcagga tgcttccgcc agtcctagaa agaggaagag ccgggcagga gagaggctct
     2221 cgcgcacacc ttgcccggct ctcaaaacgc tggcccgggg cgggggagtc ccaccccggg
     2281 ccagttggtt gcgttcagtc gcgcaccgcc gcgatcagac gcccttggtc tttacgtcca
     2341 ccaggacgaa atcggtcgcc caacccgtgg tcgggatctg ctgcggggtg acctcaaccg
     2401 gcacgggctg cgcgggcggc ttcaccggct gcgcaggaac cgttgccgca ggcggctt
//
LOCUS       DAOSCV010000688         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28037_length_2449_cov_3.201337, whole genome shotgun sequence.
ACCESSION   DAOSCV010000688 DAOSCV010000000
VERSION     DAOSCV010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28037_length_2449_cov_3.201337"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /gene="cimA"
                     /locus_tag="PLY56_15000"
     CDS             <1..1573
                     /gene="cimA"
                     /locus_tag="PLY56_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOJ22834.1"
                     /translation="KIYDTTLRDGSQAEGISFSLEDKLRIAARLDRFGIDYIEGGWPY
                     SNPKDITFFERIRETPLKHARVAAFGSTRRARLSAEDDPNLRMLVASGAPVITIFGKS
                     WDLHVIEALRVTREQNLEMIADSVRFLKAHAEEVIYDAEHFFDGYRSDPEYALATLTA
                     AAEAGADCLVLCDTNGGSLPNQVAEAIQAVRDHVRVPIGIHAHNDSETAVANSIVAVQ
                     FGAVHVQGTMNGIGERCGNANLCSVIPSLELKLGLETIGRERLRELTEVSHFIDEVAN
                     LTPNDRQPFVGRSAFAHKGGVHADAVLKLGSTYEHLAPETVGNKRKILVSELAGGSAV
                     RHKAEQFGVDLTKDSPITRAILDQVVRLENEGYSYEGAEGSLELLIRKAQGEYEKTFD
                     LISFRVIIEKRAGDEEPITEATLKLRVNGEVAHTVAEGDGPVHALDNAMRKALQQFFP
                     HLAHMRLTDFKVRVVNVREGTAAKVRVLVETTDAQETWNTVGVSTNIIEASWQALVDA
                     VDYAMMRERRPRNGN"
     gene            1697..>2449
                     /locus_tag="PLY56_15005"
     CDS             1697..>2449
                     /locus_tag="PLY56_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ22835.1"
                     /translation="MALADLVSEYVPAVDGEMAAVLASSPGSQRIAPYLRYHLGWVDQ
                     EFQPLGPAEQRRYGGKRLRPILCLLAYRSARDDWQRALPAAAVVELIQNFTLIHDDVE
                     DNDPERRHRPTVWKLWGVPHAINAGSHMQALVNAAALRLTACGVADAVVLEAVRLLTD
                     CILELTDGQYLDISYEGRFDVTLDDYFTMTGGKTGALFELSCRLGAFLARTDDPEFVD
                     AWSQVGRSFGLAFQARDDTLGVWEKSAATGKVQ"
BASE COUNT          446 a          815 c          791 g          397 t
ORIGIN      
        1 caagatctac gacacaacac tgcgtgatgg ttcacaggca gagggaatct ccttctcgct
       61 ggaggataag ctgcggatcg ccgcccggct cgaccggttt ggcatcgatt atatcgaggg
      121 cgggtggccc tactccaacc caaaggatat caccttcttc gagcgcatcc gcgagacgcc
      181 gctgaagcat gcgcgcgtgg ccgccttcgg tagcacccgg cgtgcccggc tctctgccga
      241 ggatgacccg aacctgcgca tgctggttgc cagcggtgcg cccgtgatca ccatcttcgg
      301 caagtcgtgg gatctgcacg tcatcgaggc gcttcgcgtc acgcgcgagc agaacctgga
      361 gatgatcgcc gattcggtgc gcttcctcaa ggcgcacgcc gaggaggtga tctacgacgc
      421 cgagcacttc ttcgatggct accgctccga tccggagtac gcgcttgcaa ccctgaccgc
      481 cgctgccgag gcgggcgccg attgcctggt cctgtgcgac accaacggcg gctcgcttcc
      541 caaccaggta gccgaggcga ttcaggctgt gcgtgaccat gtgcgcgtcc ccatcggcat
      601 ccacgcgcac aacgactccg agaccgccgt cgccaactcc atcgtagccg tccagttcgg
      661 cgcggtccat gtgcagggca ccatgaacgg cattggcgag cgctgcggca acgccaacct
      721 ctgctccgtg atcccaagcc tggagctgaa gctcgggctg gagacgatcg gacgcgagcg
      781 cctgcgcgag ctgacagagg tttcgcactt catcgacgag gtggcaaacc tgacgccgaa
      841 cgaccggcag ccgttcgtcg ggcgcagcgc attcgcgcac aagggcgggg ttcacgccga
      901 tgccgtcctg aagctcggga gcacctacga acacctggcg ccggaaacgg tgggcaacaa
      961 gcgtaagatc ctcgtctcgg agctggccgg cggcagcgcc gtacgccaca aagcagagca
     1021 gttcggcgtc gatctcacca aggactcgcc catcacgcgc gccatcctcg atcaggtggt
     1081 gcgcctggag aacgagggct actcctacga gggcgcggaa ggctcactgg agctcctcat
     1141 ccgcaaggcg cagggggagt atgagaagac cttcgacctc atcagcttcc gggtcatcat
     1201 cgagaagcgc gccggcgacg aggagccgat cacggaggcg acgctgaaac tgcgcgtcaa
     1261 cggcgaggtt gcgcacacgg tggccgaagg cgatggcccg gtgcacgcac tcgataacgc
     1321 gatgcgcaag gcgctgcaac agttcttccc gcatctggca catatgcgcc tcaccgactt
     1381 caaggtgcgc gtggtcaacg tgcgtgaggg caccgcagcg aaggtgcgag tcctggtgga
     1441 gacgaccgat gcccaggaga cgtggaacac cgtgggcgtc tcgaccaaca ttattgaggc
     1501 ctcgtggcag gcgctcgtcg atgccgtgga ttacgcgatg atgcgcgagc ggcgcccgcg
     1561 caacggcaac tgagaatacg cgagagccgc cgctgaaccc ggaaacgctg gcgttcaccg
     1621 gcggctctca ccgtgtttgc ccgcggctct cgcggggctt cttggcctat ccccgaggcc
     1681 agcaaaggag agaggcatgg cgcttgcgga tctggtaagc gaatacgttc ctgccgttga
     1741 tggcgagatg gcggccgtcc tggccagcag cccgggaagc cagcggatcg ctccgtacct
     1801 gcgttaccat ctgggatggg tcgatcagga gttccaaccg cttggccccg cggagcagcg
     1861 ccgttacggc ggaaagcgcc tgcgcccgat cctctgcttg ctcgcctacc gcagcgcgcg
     1921 cgacgattgg cagcgagccc ttcccgccgc cgcggtcgtg gagctcatcc agaacttcac
     1981 cctcatccac gatgacgtgg aggacaacga cccggagcgc cgccaccgcc ccaccgtgtg
     2041 gaagctatgg ggcgtgcccc acgccatcaa cgccggctcg cacatgcaag cgctggtcaa
     2101 cgccgccgca ctccgcctca ccgcctgcgg cgtcgcggac gcggtggtcc tggaggccgt
     2161 ccgtctcctc accgactgca tcctggagct gaccgacggc caatacctgg atatctccta
     2221 tgagggccgc ttcgatgtga cgctcgacga ctacttcacc atgaccggcg gcaagacggg
     2281 ggcgctcttc gagctctcat gccgcctggg cgcgttcctc gccagaacgg acgacccgga
     2341 gtttgtggat gcctggagcc aggtcgggcg ctcctttggc ctggcattcc aggcgcgcga
     2401 cgacacgctc ggcgtctggg agaaatctgc ggccacaggg aaggtgcag
//
LOCUS       DAOSCV010000689         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28043_length_2449_cov_2.651211, whole genome shotgun sequence.
ACCESSION   DAOSCV010000689 DAOSCV010000000
VERSION     DAOSCV010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28043_length_2449_cov_2.651211"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>834)
                     /locus_tag="PLY56_15010"
     CDS             complement(<1..>834)
                     /locus_tag="PLY56_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22836.1"
                     /translation="PDRACAKTPGTSFGTLWEEVRSCHSSDPLEESTLRFAEGLCAYR
                     AGLSGDARDAFEKALDVVAHVKAEDARWALASSAHWSLALALRRTGDLDEAVQRARQG
                     AREARERGAVWLEGAARLALGRVLRYAPGERERARANFDEAHRLLSAAHPADPLGVAY
                     VRAHQARLALDAGNASLALSLLRDARAGFGHCRHQRALAHSQIDQGWAHIQLRTISVA
                     RHNGEEALERLRAMGDERGVLRAWQLLGQAALEEGRIEDAIELLKQTARGASAIRDGT
                     M"
     assembly_gap    835..844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1021..1593)
                     /locus_tag="PLY56_15015"
     CDS             complement(1021..1593)
                     /locus_tag="PLY56_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22837.1"
                     /translation="MPGNPTPADPTTLVPGSPEAEAFADALLMQAKRCGLRLAAALAA
                     AARDAGDAGAGLRQAAEEIALSCAYTTERELARRRMRHVCDNGGEPGEGEEAEACGVS
                     SRLWWLSGLIATDLDHLARRLGLTQRQTAVWTATIRGETLDAIALSLSIGLSTAHAHL
                     VRAQQKARDSWLAVYSQEVRGTPCGSRRPR"
BASE COUNT          372 a          786 c          883 g          398 t
ORIGIN      
        1 gcatggttcc atcccgaatc gccgaggcgc ctcgggcggt ctgcttgaga agctcgatgg
       61 cgtcctcgat acggccctcc tcgagagcgg cctggccgag aagctgccat gcccgcagga
      121 ccccgcgctc gtctcccatg gcgcgcagcc gctccagagc ctcctcgccg ttgtggcggg
      181 cgaccgagat cgtccggagc tggatgtgcg cccaaccctg gtcaatctgg ctatgagcca
      241 gcgccctctg gtgccggcag tgaccgaagc cggcccgcgc atcgcgcaac aggctcaggg
      301 ccagagaggc attgcccgca tcgagcgcca ggcgtgcctg gtgggcgcgc acgtaagcca
      361 cgccaagagg atcggcggga tgggcggcgc tgaggaggcg gtgtgcctcg tcgaagttgg
      421 cccgggcacg ctcgcgttcg cccggagcat agcggagcac gcgccccagg gcgaggcgcg
      481 cggcgccctc cagccagacg gcccctcgct cgcgcgcctc gcgtgctccc tgacgggccc
      541 tctgaaccgc ctcgtccaga tctccagtgc ggcgaagcgc cagcgcgagc gaccaatgcg
      601 ccgaagaggc gagtgcccat cgcgcatcct ccgctttcac gtgcgcgacc acgtcgagcg
      661 ccttttcgaa ggcgtcgcgc gcgtcgcccg ataggcccgc tcgataggcg cacagcccct
      721 ccgcgaagcg gagcgtactc tcctcgagtg gatccgagga gtggcacgag cgaacctctt
      781 cccagagcgt cccaaaggag gtgccggggg tcttcgcgca ggcccggtcc ggccnnnnnn
      841 nnnncggcca cagcagcagc gagcggatgc cgctctcgac ggcgctgtcg cgcaggtggc
      901 gtaccgggtc gcggaggccg gcgggtaggg actgctccat gggtagagtg ctcgatcgtc
      961 gcatcatctc gtgagcgatc tttcgcgccg gcgcccgccg atcccttcct catctggcgc
     1021 ctatcgcggg cgccggctcc cgcagggcgt accacgcacc tcctgggaat agacggctag
     1081 ccatgagtcg cgcgccttct gttgggcgcg aacgagatgg gcgtgagccg tggataggcc
     1141 aatgctgagc gacagggcga ttgcatccag ggtctcgccg cggatggtgg cagtccacac
     1201 cgctgtctgg cgctgtgtga ggccgaggcg gcgtgcgagg tggtccagat cggtggcgat
     1261 caggccgctg agccaccata ggcgcgatga aaccccgcac gcctccgcct cttctccctc
     1321 tcccggttcg ccaccgttat cgcacacgtg gcgcattcgg cggcgggcca gctcacgttc
     1381 tgtcgtgtag gcgcaggaga gcgcgatttc ctcggctgcc tggcgcaggc cggcgccggc
     1441 atcgcccgcg tctctggcgg cagcggcaag cgcggccgcc aggcgcaggc cgcaccgttt
     1501 ggcttgcatg agaagcgcat cggcgaacgc ttccgcctcg gggctccccg gcaccagggt
     1561 ggtcgggtcg gccggggtgg ggttgccagg catcgggacg atctcccccg gaagcagcat
     1621 ggggcgccgg cacggctccg gtaccggggg acgggatgcg ctcctggcgc tgtggtgctt
     1681 cgggtgcgat gggtgcaggc gcgcgcgcgc ggaaccgcgg atcctgtggc aggacatggc
     1741 cgttctcctt ccccgctttg ggcggatggc acacatgcgt ttgctaaact ggcgccatta
     1801 taccagccgc gtgcgaatgt gtcaagcgtt agttcgaata tttcgaacgc agtgcggatt
     1861 gtgggcaggc tccgggttgg ggaaggggtg agataaggat tccgggctgc ggcgcgcgaa
     1921 ccaggatcgc gaggggattc cgcaagagga gatccggagc atcgaaagca gcgcgcaatc
     1981 ctgcgggcgg cgatgataga agttgaagaa taaagcgcat ttcgccgtgc gaagcatcgc
     2041 tcttgggatt ctccaatata agcaaggtcc gggcggttgc ccgcgttctc cagcatgcgc
     2101 cctgccttcg ataccacgga tgtggtcgtg gctatgtatg ccggtgcatg acccagggtg
     2161 cggacaccct gggctaagat gtgcggcccg ttcggggccg gggcagggca gccggcaggg
     2221 cgatgggaga agaggtcgga gaggtccggg cattggaggc gggagtcgct gtgcccagat
     2281 gggattcctt cgagctccgc gcacgagggc tcgcgatact cccgggtgtc tcagaacggc
     2341 gcgggctgga agagggatgc gtcgcgcggc gataaatcac cgcgctgctt gtgagcgaag
     2401 ccctttcggg ctgggttggg tggccatccc gggggtccta gcccgcatt
//
LOCUS       DAOSCV010000690         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28070_length_2447_cov_3.902592, whole genome shotgun sequence.
ACCESSION   DAOSCV010000690 DAOSCV010000000
VERSION     DAOSCV010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28070_length_2447_cov_3.902592"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..916
                     /locus_tag="PLY56_15020"
     CDS             227..916
                     /locus_tag="PLY56_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22838.1"
                     /translation="MSRARVLIIEDEAHIVDVLTWYFQREGWEVLSAHDGITGLELAR
                     QQAPDLIILDLMLPGLDGMEVCRRLRQESRTPILMLTARDAEMDKITGLEIGADDYVT
                     KPFSSREVVARVKALLRRASYSEPSRDEPLVFPGLVIDRAGKEVSVNGRPVRLTPTEF
                     QVLLLLASHPGCAFSRDEIIQHVTGVEYIDTRTVDVHIRHLRAKLGDDPSRPRYLHTV
                     WGTGYKFEALT"
     gene            945..>2447
                     /locus_tag="PLY56_15025"
     CDS             945..>2447
                     /locus_tag="PLY56_15025"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22839.1"
                     /translation="MFRSFRAQLVVSFFLVILVTPLLFISLFYGPFGRYLRSGNHGSS
                     LPYHARVVGNVVQDALAKGASVDELDHLLRVAAEGTPYQFVLIDRDRQVMVDTGVAPG
                     SALPANPARATNYGRPLAPDGRLAVQREGRLLRAAAPINLEGEPFGVLLVSRFSQRPS
                     YFSPSTFIPRIILGLVIAGILGLALSRRLLRPVDALTRAAEAMAGGDLSQRVAIPPHT
                     ELGRLSERFNHMADRIEELVSRLSGEHERLQIAHACLAESERRQRELIADVSHELRTP
                     LAVIRSSVDALVDGVADNGERQQRCLRAIEEETTALSRLVEDLLTLSRIDSGQLPLRR
                     EPVSVAALFERSIRRFLPEAEAHGIVLESSLAEGLFFMGDEERMAQVLGNLLDNAIRH
                     TPAGGRVRLEAAREAERIRIRVSDTGCGIAPEHLPRLFDRLYRAERSRCKGSGGSGLG
                     LAIVHEIVDAHGGTVEAESEPDKGMSVTIYLADGAALGFRDKEMDGAGRPH"
BASE COUNT          401 a          798 c          815 g          433 t
ORIGIN      
        1 cgagcgaagc cctgccgggc tgggttgggg atactcccag atgtctcaaa acggcagtag
       61 ctgagaggag aagcaccgaa ggggctttgc cggaaagcag cgcggagctt cgagctccgc
      121 gcgcgcgggc gccctctcct tatgccggaa tcccaagagt gccgggcagc gatttgcatc
      181 cccaccgccg gcatgataca ataggcacga agagaggcat tgactcatgt cccgcgcccg
      241 tgttctgatc atcgaagatg aggctcacat cgtcgatgtg ctcacctggt acttccagcg
      301 tgagggctgg gaggtgctgt ctgcgcatga tggcatcacc gggctggaac tggcgcgcca
      361 gcaagcgccc gatctgatca tcctcgatct gatgctgcct ggtctggatg ggatggaagt
      421 ctgccgccgg ctccgccagg aatcgcgcac gcccatcttg atgctcaccg cccgcgatgc
      481 ggaaatggac aaaatcacgg gcctggagat cggcgcggat gattacgtca ccaagccgtt
      541 cagctcgcgc gaggtggtcg cccgagtgaa agccctcctg cgccgcgcca gctactcgga
      601 gccgtctcgc gacgagccgc tcgtctttcc cgggctcgtc atcgaccgcg ccggcaagga
      661 ggtctctgtc aacggtcgcc cggtgcgcct gacgccgacg gaattccagg tactgctcct
      721 cttggctagc cacccgggct gcgccttcag ccgcgatgag atcatccagc acgtcaccgg
      781 ggtcgagtat atcgatacgc gcaccgtgga cgtgcacatc cgccacctgc gcgccaagct
      841 gggagacgac ccctcgcggc cgcgctactt gcacaccgtc tggggaacgg gctacaagtt
      901 cgaggccctc acgtagtggc gttggactgc aaagagaggc acccatgttc agaagctttc
      961 gggcgcagtt ggtggtcagc tttttcctcg tcatcctggt gacgccgctc ctcttcatca
     1021 gcctcttcta tgggccgttc gggcggtatc tgcgctcggg gaaccacgga tcctcgctgc
     1081 cctaccatgc gcgggtggtt ggcaacgtgg tgcaggatgc gctcgcaaag ggtgcttcgg
     1141 tggacgagct cgaccatcta ctgcgcgtgg ctgccgaagg aacgccctat cagttcgtgt
     1201 tgatcgaccg cgacaggcag gtgatggtgg ataccggcgt ggcgccgggc agcgcgcttc
     1261 ccgcgaaccc ggcgcgcgcg accaactatg gccggccgct cgcccccgat ggccggctcg
     1321 ccgtgcagcg ggagggccgg ctccttcgcg ccgccgcgcc aatcaatctg gagggggagc
     1381 ccttcggggt gcttctcgtc agccggttca gccagaggcc gagctatttt agcccctcaa
     1441 ccttcatccc gcggatcatc ctggggctcg tgatcgcggg gattcttggt ctggctctct
     1501 cccgccgcct gcttcgccct gtcgatgccc tcacccgcgc cgccgaagcg atggccggcg
     1561 gggacctgag ccagcgcgtc gccataccgc ctcacaccga gttggggcgg ctctcggagc
     1621 ggttcaacca catggcggat cggattgagg agctggtctc gcggctctca ggcgagcacg
     1681 agcgcctcca gatcgcacac gcgtgcctcg ccgagtccga gcgccggcag cgcgagctga
     1741 tcgccgacgt ctcgcatgag ttgaggacgc ctctcgccgt catccgctcc tctgtcgatg
     1801 ccctggtgga tggcgtggca gataacgggg agaggcagca gcgctgcctg cgcgccatcg
     1861 aggaggagac gaccgccctt tcccgtctgg tggaggacct gctcacgctc tcccgaatcg
     1921 acagcggtca actcccgctg cgccgcgagc cggtgagcgt cgccgccctc ttcgagcgga
     1981 gcatccgccg gtttctccca gaggccgagg cacacggaat tgttctcgag agctccctgg
     2041 ccgaggggct cttcttcatg ggcgatgagg agcgcatggc gcaggttttg ggcaacttgc
     2101 tggataacgc gatccggcac accccggccg ggggccgcgt ccggctagag gccgcgcgag
     2161 aggcagaacg gatccggatc cgggtatccg atacgggatg cggaattgcc cccgagcatc
     2221 tgccccggct tttcgaccgc ctctaccgcg cggagcggtc gcgctgcaag ggctccggag
     2281 gctcggggtt gggcctggcg attgtccatg agatcgtcga cgcgcatggc gggacggtgg
     2341 aagcagagag cgagccggac aaaggcatga gcgtcacgat ctacctcgcg gacggggctg
     2401 ctttgggatt ccgagataag gagatggatg gagcgggccg cccacac
//
LOCUS       DAOSCV010000691         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28073_length_2447_cov_3.455268, whole genome shotgun sequence.
ACCESSION   DAOSCV010000691 DAOSCV010000000
VERSION     DAOSCV010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28073_length_2447_cov_3.455268"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLY56_15030"
     CDS             <1..185
                     /locus_tag="PLY56_15030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ22840.1"
                     /translation="GVTEAEHGGDLFGFERLEAAVQEWQGDHPQELVDHIARRVREFA
                     GGRIRDDLALLAIRWR"
     gene            complement(254..1633)
                     /gene="hpnE"
                     /locus_tag="PLY56_15035"
     CDS             complement(254..1633)
                     /gene="hpnE"
                     /locus_tag="PLY56_15035"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOJ22841.1"
                     /translation="MALSQRRGDAAIASVIVIGGGLAGLSAACALADLGFAVSLIERR
                     SLLGGMATSLRVRGLPEPVDNCQHVLMRSCTALLDFYARIGVRDQVRFDPEIAFIDER
                     GAVSSLAAARLPAPLHLAPGFARLRFLTLRDKLAIARAFLGALPGGAGSSEESFAHWL
                     QRRGQTERTVDAFWRPVIASALNEEPERVSASYALLVLRETFLARRDGWELGVPKRPL
                     TQMHAAPAETYLSQRHARLLYRAEATRLQVEGDAVTGVLLADGTRLRADYYLSAVPYP
                     ALFRLLPPQLVGHPFFSRPRGMQPSPIVAAHLWLDRPVTPLPHAVLLGRSIHWFFNKT
                     HDFGPRDGGFHLALVTSAAHALVSRPRQDLLRQLFEEIRDVLPEARYARLLHAVVFKE
                     RCATFAPLPGCDTLRLPAQTPLRNLFLAGDWTHTGWPATMEGAVRSGMNAARTIAAAS
                     GLSPAQQFI"
     gene            complement(1588..>2447)
                     /locus_tag="PLY56_15040"
     CDS             complement(1588..>2447)
                     /locus_tag="PLY56_15040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOJ22842.1"
                     /translation="YRHCARVTRRCAQSFYWGLRLLPREKRRALYAIYAFLRGCDDSA
                     DEAAPDARPGQLHRWRRALDAALCGECAGDPCLPAFSDAVARYAIPGRYFHLLLDGAE
                     MDLSVRRYPTFEALYDYCYRVASTVGLICLHVWGYEGEGAELAAEECGIAFQLTNILR
                     DIREDAARGRIYLPEEDLRRFNYGEREIAGQVRDARFAALMAFQVERARRYYRSVDRL
                     LPCVSPDSRACLMAMAGVYEGLLDCMERSGFDVYSRRIALPKSHKIAIALRAWWRYRN
                     GGETRPSQA"
BASE COUNT          444 a          775 c          883 g          345 t
ORIGIN      
        1 acggggtcac cgaggcggaa catggcgggg atctcttcgg gttcgagcgc cttgaggccg
       61 ccgtgcagga atggcaaggc gaccaccccc aggagttggt ggaccacatc gctcggcgcg
      121 tacgcgagtt cgccggcgga cgcatccgcg acgacctggc cctcctggcg atccgctgga
      181 ggtaagatcc gcagatggag agtggtttcc acagatgggc acagatatat agataggcac
      241 agacaatctg aaattagatg aattgctggg ccggtgagag tcctgacgca gcggcgattg
      301 tccgggcggc gttcattccg ctgcgaaccg cgccctccat cgtggccggc cacccggtat
      361 gggtccaatc gccggccagg aagaggttgc gcagtggcgt ctgcgccgga agccgcaacg
      421 tatcgcatcc cggtaggggc gcgaacgtgg cacaccgctc cttgaatacg acggcgtgca
      481 gcaggcgcgc gtatcgcgcc tcgggcaaga cgtcgcggat ctcctcgaac agctgccgga
      541 ggaggtcttg ccggggcctg ctcaccaggg catgcgcggc gctggtgacc agagccaggt
      601 ggaagcctcc atcgcggggc ccgaaatcgt gcgtcttgtt gaagaaccag tggatggaac
      661 gcccgaggag caccgcgtgg ggcagggggg tgaccggccg gtccagccag agatgggcgg
      721 ccacgatggg ggaaggctgc atgccccgag gacgcgagaa gaacgggtgt ccgaccagct
      781 ggggcgggag aaggcgaaag agcgcggggt agggcacggc gctgaggtag tagtcggcgc
      841 gcagccgcgt tccatccgcg agaaggacgc cggtgactgc gtcgccctcc acctggaggc
      901 gggtggcctc tgcccggtag agcagacgcg cgtgccgctg agagaggtag gtctccgcgg
      961 gtgccgcatg catctgtgtg agcgggcgtt tgggcacgcc tagttcccat ccgtcgcggc
     1021 gcgcgaggaa cgtctcgcgg agaacgagca aagcgtagct cgcgctgacg cgctccggct
     1081 cctcgttcag cgcgctcgca atcactggcc gccagaaggc atcgaccgtc cgttctgtct
     1141 gtccgcgacg ctgtagccag tgggcgaagc tctcctccga ggatccggcc ccgcccggta
     1201 gggcacccag gaacgccctg gcgatcgcga gcttatcacg gagcgtgagg aagcggagcc
     1261 gcgcaaagcc gggcgccagg tgtagaggag cggggagcct ggcggcggcc agggatgaga
     1321 cggcccctcg ctcgtcaatg aaggcgatct cgggatcgaa acgaacctgg tcacgcacgc
     1381 cgatgcgcgc gtagaaatcg agcagcgccg tgcagcttcg catcagcacg tgctggcagt
     1441 tatcaaccgg ctcgggcagg ccacggaccc gtagggaggt ggccatcccg ccaaggagag
     1501 agcggcgctc gatcagggag accgcgaagc ccagatccgc cagggcgcac gccgccgaga
     1561 ggcccgccag cccgccgccg atgacgatca cgcttgcgat ggccgcgtct cccctccgtt
     1621 gcgatagcgc caccacgccc gcagggcaat ggcgatcttg tgtgacttcg gcagggcgat
     1681 ccgtctggag tagacatcaa agccgctgcg ctccatgcag tcgaggagac cctcatacac
     1741 gccggccatg gccatcaggc aggcgcggct gtccggcgag acacacggga gcaggcggtc
     1801 gacgctccgg taatagcggc gggcgcgctc cacctggaac gccatcaacg cggcgaaacg
     1861 cgcgtcgcgc acttgcccgg ctatctcccg ctcgccatag ttgaagcgcc gaaggtcctc
     1921 ctctggcagg tagatccgcc cccgggcggc atcttcgcga atgtcgcgca atatgttggt
     1981 gagctggaag gcgattccgc actcctcggc cgcgagttcc gcgccctcgc cctcgtatcc
     2041 ccagacgtgc aggcagatca ggccgacggt ggatgccacc cggtagcaat agtcgtagag
     2101 cgcctcgaag gtggggtagc ggcggaccga gaggtccatc tccgcgccat ccaggagcag
     2161 gtggaagtag cgcccaggga tagcatagcg agcgacggcg tcgctgaagg ccgggaggca
     2221 gggatcgccc gcgcattcac cgcagagcgc ggcatcgagg gcgcgtcgcc accggtgaag
     2281 ctggcccgga cgcgcgtcgg gagcggcctc atcggcgctg tcgtcgcagc cgcgcaagaa
     2341 ggcgtagatg gcgtagagcg cgcgtcgctt ctcgcgcggc aggaggcgca gcccccaata
     2401 gaagctctga gcacaccggc gggtcacccg ggcgcagtgg cgatagg
//
LOCUS       DAOSCV010000692         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28088_length_2446_cov_3.468841, whole genome shotgun sequence.
ACCESSION   DAOSCV010000692 DAOSCV010000000
VERSION     DAOSCV010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28088_length_2446_cov_3.468841"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLY56_15045"
     CDS             complement(<1..959)
                     /locus_tag="PLY56_15045"
                     /inference="COORDINATES: protein motif:HMM:NF020698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HOJ22843.1"
                     /translation="MRAVRRISFFFCLLVLLVIPRSPGLAQGLPGEPEVILLRADPEV
                     IAADGQSSATLLAEVRDRQGAFVVDGTIVRFTTTAGVISPTAETRSGVVRVMLRSSAE
                     PAVAEVTATAGTRAVARVRVTFSSEAMAAQQVAPFARIQAQSLHYVPDKDLIDAAGDV
                     RLTYRGVFIIADRLQLHVGSLRVVAQNNIRVRCAGREVTADRLMLEMPQMQGRLLAVE
                     ENGTIRRQMVSGYALDLFAAEGPTPEGVFDLQDAETDKALISARKITLFPGDKVQFSG
                     FGLRVAGAHIIWLPFYVLSLNPYGPDADHLVSLDSMGGISVNLP"
     gene            complement(1317..>2446)
                     /locus_tag="PLY56_15050"
     CDS             complement(1317..>2446)
                     /locus_tag="PLY56_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ22844.1"
                     /translation="RRAPAGVGLRYSVTATMLDSSNGILESEAGEQVDQTPVMVVRRL
                     APPANFTATGGATVEGLPQVTFNWTPSTDPSALGYVIYYSDQQAGPYTRVFAPADGTG
                     KATVAATESSYILTQFVLPALENDKAFWFRIATVDKLGIEGTPSAEVRAVPNPVPPKP
                     LNVTVTNRDGSGNLLDMGVQVSWSAPVGGAAPAITGYRVYRSKYAAGPFELVTPVPVP
                     ASATPSFLDQFLNGSVSLPEAHDQDFYYRVVALVNYSVAGVVEGEVSDVAGPVRVMNV
                     PPGKVTIRPATATNGIVLVRWDALRDLQNNPVRDLARYTVSRREKQDLTGETEQFFPV
                     GPEMTEFYDTTATAGTTYVYRVQAVDFIHEGPWSDTVEVTP"
BASE COUNT          447 a          869 c          743 g          387 t
ORIGIN      
        1 ggaaggttca ccgagatgcc gcccatgctg tcgaggctga ccaggtgatc cgcatcgggg
       61 ccatatgggt tgagcgagag gacgtagaat ggaagccaga tgatgtgcgc cccagccacg
      121 cgcagcccga acccggagaa ctgcacctta tcgcctggga agagcgtgat cttccgcgcg
      181 gagattaggg ccttatctgt ctcggcatcc tggagatcga acactccctc gggcgtcggc
      241 ccctcggccg caaagagatc gagcgcgtag ccgctgacca tctggcgccg gatcgtgcca
      301 ttctcctcca ccgccagcag ccggccctgc atctggggca tctccagcat cagccggtcc
      361 gcggtgacct cgcggcccgc gcagcgcacg cggatgttgt tctgcgccac cacacgaaga
      421 gagccgacgt gcagctgcag gcgatccgcg atgatgaaca ccccgcgata cgtcaggcgc
      481 acgtcacccg cggcatcgat cagatcttta tctggaacgt aatggagcga ttgggcctga
      541 atgcgggcaa acggagcaac ctgctgagcc gccatcgctt cgctggagaa ggtgacgcgc
      601 acgcgagcaa cggcgcgcgt tcccgccgtc gccgtcacct cggcaaccgc aggctccgcg
      661 ctgctccgaa gcatcaccct taccacgccg gagcgcgtct cggccgtagg gctaattacg
      721 cccgccgtcg tcgtgaaccg gacgatggtg ccatcaacga caaatgcccc ctgccggtca
      781 cgtacctcgg cgagcaacgt cgctgacgat tggccgtctg cggcgatgac ctccgggtcc
      841 gctcggagga ggatcacctc cggttcgccc ggcagaccct gagccaggcc cggggagcgc
      901 gggatcacca ggagcacgag caagcaaaag aagaacgaga tgcggcgaac ggctctcatg
      961 ctctcatgtc gcaagggaaa caccagcgca cccggcaccg gtgggaagat agtcaactgc
     1021 ctccggctat gagcgcgagc cggacgcctg cttccgcccg ccacgctcac tctgaaggat
     1081 atctactgtt gtaagcattc gccgccccgg gccccggcac cttctccaac accaaacaga
     1141 acgccagccc ccttctgccc ggccctgtcg ccgatccaca gatcgccctg ggcccacccc
     1201 ctgccccatc cataccctga caaacgccga aggggccggt cgaatgaccg gcccccagcc
     1261 tattccattc ggagccgccg cggacggcgg ccccgcctat cctcgctccg agtgacctac
     1321 ggcgtcactt ccaccgtatc cgaccacgga ccctcgtgga tgaagtccac cgcctgcacg
     1381 cgatacacat acgtggttcc cgcagtcgcc gtagtatcgt agaactcggt catctccggc
     1441 ccgaccggga agaactgctc cgtctcgccc gtcagatcct gcttctcgcg gcgcgagacg
     1501 gtgtagcgtg ccaggtcgcg gaccgggttg ttctgcagat cccgcaaggc atcccaccgg
     1561 accaagacga tcccgttggt agcggtcgcc gggcgaatcg tgaccttacc aggaggcacg
     1621 ttcatcacgc gcaccggccc ggccacatcg ctgacctcgc cttccaccac gccggccacg
     1681 ctgtagttca ccagcgccac cacgcggtag tagaagtcct gatcatgcgc ctcgggaagc
     1741 gacaccgacc cgttcaggaa ctgatccagg aagctcggtg tcgccgacgc cggtacgggc
     1801 accggggtca ccagctcgaa cgggcctgcg gcatacttgc tgcgatatac ccggtaaccg
     1861 gtaatcgcgg gcgcagcacc acccacaggc gcagaccagc tcacctggac gcccatatcg
     1921 agtagattgc cgctgccatc ccggttggtg acggtcacgt tcagcggctt gggcggcacc
     1981 gggttgggca cagcccgcac ctcagcgctc ggggtaccct cgatccccag cttatcgacg
     2041 gtggcgatcc ggaaccagaa cgccttgtca ttctccaggg ccgggagcac gaactgcgtc
     2101 aggatgtacg acgactcggt ggcggcgaca gtcgccttgc ccgtgccatc ggccggcgcg
     2161 aagacgcgcg tgtatggccc tgcctgctgg tccgagtagt agatcacgta gcccagcgcc
     2221 gagggatccg tcgacggcgt ccagttgaac gtcacctgcg gcaggccctc gacggtggcg
     2281 ccgccggtcg ccgtgaagtt cgcgggcggc gcaagccgac gcaccaccat cacgggcgtc
     2341 tgatccacct gctcgccggc ttcgctctcc aggatgccgt tgctgctatc gagcattgtg
     2401 gccgtcaccg agtagcggag gccaacgcct gccggcgccc tgcggt
//
LOCUS       DAOSCV010000693         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28107_length_2445_cov_3.628033, whole genome shotgun sequence.
ACCESSION   DAOSCV010000693 DAOSCV010000000
VERSION     DAOSCV010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28107_length_2445_cov_3.628033"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PLY56_15055"
     CDS             complement(<1..672)
                     /locus_tag="PLY56_15055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22845.1"
                     /translation="MFRFLHEVLNRVNVAYVATYPPAECGIATFTRDLVTAIQKFTPF
                     SEPRVVAINEEGHILRYDHRYVKHTISKQDKESYQEAAEYINSQPVDVVSIQHEFGIF
                     GGPEGEYLIDFLAALRKPIVTTLHTVVLQPWGTMRAGVEAIFEHSDVVVAMLDIARDI
                     LDDVYKVDTRKLRIIPHGVPNVPRIPIEVAKKNLGLDGHKIISTFGLVSRGKGLEYVI
                     QAMPEI"
     gene            complement(690..1316)
                     /locus_tag="PLY56_15060"
     CDS             complement(690..1316)
                     /locus_tag="PLY56_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22846.1"
                     /translation="MQRLRYARIEAGHRHRCRVACAAAGLIATLVCTPVVTRPAHAAP
                     ADPEATATAVLDQVVDGLWERSDHFFHHGDYETTTRINRLVVRMDPHFVEGLNTLAWL
                     LRQGLNRPQEALALHHRAIRDNPERWEAFFDLGMFHFDRKDYPLAAHYFRRSVELEAP
                     AAKQHMLAHTYEKMGRTADALRAWRAIVEKFPDDQPARRNLERLEKEE"
     gene            complement(1288..2025)
                     /locus_tag="PLY56_15065"
     CDS             complement(1288..2025)
                     /locus_tag="PLY56_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22847.1"
                     /translation="MFLVVRSAFRPLHAGLLLLSLLMALDPPVRAVGLFGLAVYAILV
                     CYDVWGSSRAWATAAEIEPEQIAEPYAGQLREAEAARRRIHEAADGAGHSLARVMAPI
                     KADADQVVARLRALAERATRIDRFLKRENREQLEKSLAALEEQASATDDLFARDQYLQ
                     ARQAREEQLRDYEELSLCLARVQAQIANVLSSLEAAEAKVIKLQAADLRHAGAVTDAV
                     TRSLQELSLEMTGLQESVDATIAIRAH"
BASE COUNT          438 a          788 c          754 g          465 t
ORIGIN      
        1 gatctccggc atcgcctgga tgacgtactc cagccccttc ccgcggctca ccaggccaaa
       61 ggtggagatg atcttgtgcc catcgagccc caggttcttc ttggcgactt caatcggaat
      121 tcggggtacg ttcggaacgc cgtgcgggat gatgcgcagc ttccgtgtat ccaccttgta
      181 cacgtcgtcc aggatatcgc gagcgatatc gagcatcgcc accaccacat cggagtgctc
      241 gaagatcgcc tcgacccccg cgcgcatggt gccccacggc tggagcacca ccgtgtgcag
      301 cgtggtgacg atcggcttgc gcagcgcagc cagaaaatcg atcaggtatt cgccttccgg
      361 accgccgaag ataccaaact catgctggat gctgacgaca tcaaccggct ggctgttgat
      421 gtactccgcc gcttcctggt aactttcctt atcctgcttc gagatggtgt gcttgacgta
      481 acggtgatcg tagcgcagga tgtggccttc ctcattgatt gccaccacgc gtggctctga
      541 aaatggggtg aacttctgga tggcggtgac aagatcacgc gtgaacgtgg cgattccgca
      601 ctcggccggg ggataggtcg ccacgtaggc gacgttgacc cggttgagta cctcgtgcag
      661 gaatcggaac attcctagat cttctactct cactcctctt tctccagtcg ctccaggttt
      721 cggcgcgcgg gctggtcgtc cgggaacttt tctacgatgg cgcgccaggc gcgaagcgcg
      781 tctgctgtgc ggcccatctt ctcgtaggtg tgcgccagca tgtgctgctt ggccgcaggg
      841 gcctccagct cgacgcttcg gcggaaatag tgcgcggcta gggggtagtc cttcctatcg
      901 aaatggaaca tccccagatc gaagaacgct tcccaacgct ccgggttatc gcggatagcc
      961 cggtggtgaa gggcgagcgc ctcctgcggc cgattcagcc cctggcgcag gagccacgcc
     1021 agtgtgttga gaccctcgac aaagtgcgga tccatccgga cgaccagccg gttgatccgc
     1081 gtcgtcgttt catagtcgcc atggtggaag aaatgatccg agcgttccca tagcccatcg
     1141 acgacctgat cgaggaccgc agttgctgtc gcttccgggt ctgccggcgc ggcgtgcgcc
     1201 gggcgagtca ccacaggcgt acagaccagg gtggcgatca gccctgccgc ggcacatgcc
     1261 acgcgacacc gatgcctgtg cccagcctca atgcgcgcgt atcgcaatcg ttgcatcgac
     1321 gctctcctga agccccgtca tttcgagcga tagttcctgc aggctgcgcg tcaccgcgtc
     1381 ggtaacggcg cccgcatgcc gtaggtccgc cgcttgcagc ttgatcactt ttgcctctgc
     1441 ggcctcgagg cttgagagga cgttggcgat ctgagcttgc acgcgcgcga ggcagagcga
     1501 gagctcttca tagtcgcgca gctgctcctc gcgggcctga cgtgcctgca ggtactggtc
     1561 gcgagcgaaa agatcatccg tggcgctcgc ctgctcctcc agcgccgcca ggctcttctc
     1621 tagttgttcc cgattctcgc gcttgagaaa ccggtcgatt cgcgtggcgc gctcggccag
     1681 cgcacgcagg cgcgccacca cctgatccgc atcggccttg atgggggcca tgacgcgcgc
     1741 gagagagtgc cccgcgccat ccgccgcctc gtggatccgc cgccttgccg cctcagcttc
     1801 ccgaagctgc ccggcatacg gctccgcaat ctgttcgggc tctatctcgg cggccgtagc
     1861 ccacgctcta ctcgagcccc agacatcgta acacaccagg atcgcgtaaa ctgccaaacc
     1921 aaagaggcca accgcacgaa cgggcggatc gagggccatc agaagcgaga ggaggaggag
     1981 gccggcatgt agcggccgaa acgcggatcg aacaacaaga aacaacgaat tccttcgggg
     2041 cttgctctcc atgtcatccc tcacggaaga tggatgcgca ggggaaagag tgcccccagc
     2101 gcctccgggc ctcctctcgg attcgtttcg ccaatagagt gctctcgcgc gaggcgtacc
     2161 accccacgga gcgcacacgg acgcgttctg gtgcgtcaca ccctattata ccatagggcc
     2221 ggaaggggcg gcaagctcgc taccacgcat gaaaggagcg cgcacggatg ggggtcaggc
     2281 gcggcgttcg ccctggcgtg ggtgtttgcg cccggtcggc ggcgtgcccg cgccccggcg
     2341 ccggtctgaa cgcttgctag ggcaggagaa gcagcgatgg tcgcgaacaa cccggagtgt
     2401 gagtactgtg acctacagga tctgctacca ggtgacccgt gccct
//
LOCUS       DAOSCV010000694         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28259_length_2437_cov_2.915617, whole genome shotgun sequence.
ACCESSION   DAOSCV010000694 DAOSCV010000000
VERSION     DAOSCV010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28259_length_2437_cov_2.915617"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLY56_15070"
     CDS             <1..238
                     /locus_tag="PLY56_15070"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ22848.1"
                     /translation="AKRALFRPLGEHSQDPDDARLERELLERVNATGLGPSGFGGRIT
                     ALAVNVESYPCHITALPVAVNVQCHAARHKEAVL"
     gene            247..822
                     /locus_tag="PLY56_15075"
     CDS             247..822
                     /locus_tag="PLY56_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HOJ22849.1"
                     /translation="MSEAIKLTTPLREEQLLALRAGDRVLITGEVITARDAAHKRLVE
                     CIERGEPLPVELEGQIIYYVGPTPAPPGRPIGSAGPTTATRMDAYVPALLARGLRGMI
                     GKGRRTPAVVEALREHHAVYFGAIGGLGALLSERIRAAEVVAYEDLGTEAIRRLVVEE
                     FPAIVINDCYGGDFYREGVEQWRLPGGGGLR"
     gene            819..>2437
                     /gene="sdhA"
                     /locus_tag="PLY56_15080"
     CDS             819..>2437
                     /gene="sdhA"
                     /locus_tag="PLY56_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOJ22850.1"
                     /translation="MTHQFDAVVVGAGGAGLWAALELSGTANTAVLSKLYPTRSHTGA
                     AQGGIGAALGNMEEDHWEWHAFDTVKGGDYLCDQDAVEVMCREAIDTVYELEHRGLPF
                     SRTEDGRIAQRPFGGHSREFGKGPVRRSCYAADRTGHMILQTLYQQCIKNQVRFFDEF
                     HVVDLLIEENVCRGVIAYCLADGTLHTFRAKAVILATGGCGRMFRITSNAYALTGDGM
                     ALAYRRGVPLEDMEFFQFHPTGIYKMGILITEGARGEGGVLRNARGERFMERYAPTMK
                     DLAPRDMISRFIYREIREGRGIDGQDYVHLDLTHLPAETIETRLPDITDFVRTYLGVD
                     PVSQPIPVQPTAHYVMGGIPTDLDARVVIDEKNTPLPGLYAAGECACVSVHGANRLGT
                     NSLVDILVFGRRAGKHAASYVREVELPPLPRDPQARARAEIERLLANTGDERPIAIRD
                     ELRRVMMEDAGVVRTGESLQRVIEKLGELKERYARVGVRDQSKQFNTALLETVELGYL
                     LELAEVLAKSAFAREESRGAHYREDFPNRNDT"
BASE COUNT          401 a          741 c          885 g          410 t
ORIGIN      
        1 ggccaagcgg gcgctcttcc ggcctctggg cgagcatagc caggatcccg atgatgcccg
       61 gctggagcgc gagctcctgg agcgagtgaa cgccacgggc ctggggcctt ctggctttgg
      121 ggggcggatc accgcgctcg ccgtgaacgt ggagagctat ccgtgccaca tcacggcgct
      181 gccggtggcg gtgaacgtgc agtgccacgc ggccaggcat aaggaagcgg tgctgtaggg
      241 agagcgatgt cggaagcgat caaactgaca accccgctcc gagaggagca gctcctcgcg
      301 ctgagggcgg gcgaccgcgt tctgatcacg ggtgaggtga tcaccgcccg cgacgcggcg
      361 cacaagcgct tggtggagtg catcgagcgc ggcgagccgc tgccggtgga gctggagggg
      421 cagatcatct actacgtggg cccgacgccc gcgcccccag gacggcccat cggctcggcg
      481 gggccgacga cggcgacgcg catggatgcc tatgtgccgg cgctgctggc gcgcggcctg
      541 cgggggatga ttggcaaggg caggcgcacg ccggcggtgg tggaggcgct gcgcgagcac
      601 cacgcggtct attttggcgc cattggcggg ttgggcgccc tcctctccga gcggatccgt
      661 gccgccgagg tggttgctta cgaggatttg ggcaccgagg cgatccggcg cttggtggtc
      721 gaggagttcc ccgcaattgt gatcaacgat tgttatggtg gcgatttcta tcgggagggt
      781 gtggagcagt ggcggttgcc gggcggtggg ggactgagat gacgcatcag ttcgatgcgg
      841 tggtcgtagg tgctggcggc gccggattgt gggcggcgct ggagctttcg gggacagcca
      901 acacggcggt gctgagcaag ctctatccga cgcgctcgca tacgggcgcg gcacagggcg
      961 ggattggcgc ggcccttggc aacatggagg aggatcactg ggagtggcac gccttcgata
     1021 cggtcaaagg cggcgactat ctctgtgatc aagatgccgt ggaggtgatg tgccgcgagg
     1081 cgattgatac cgtctacgag ctggagcacc gcggcctccc cttcagccgg accgaggatg
     1141 gccgcattgc ccagcgtccc tttggcggac attcgcgcga gttcggcaag ggcccggtcc
     1201 gccgctcttg ctacgcggcc gaccgcaccg ggcacatgat cttgcagacg ctctaccagc
     1261 agtgcatcaa gaatcaggtg cgcttcttcg atgagttcca cgtggtcgac ctgctcatcg
     1321 aagagaatgt gtgccggggc gtcatcgcct actgcctggc cgatggcacg ctgcacacct
     1381 tccgtgccaa ggcggtgatc ctggccacgg gcgggtgcgg gcgcatgttc cggatcacga
     1441 gcaacgccta tgccctcacc ggcgatggga tggcgctcgc ctaccgccgc ggggtgccgc
     1501 tggaggatat ggagttcttc cagtttcacc ccaccggcat ctacaagatg ggcatcctga
     1561 tcaccgaggg cgcgcgcggc gagggcggcg ttctgcgcaa cgcgcgcggc gagcgcttca
     1621 tggagcggta tgcgcccacg atgaaggacc tggccccgcg cgacatgatc tcccgcttca
     1681 tctatcgcga gatccgcgag gggcggggca ttgatggcca ggattacgtc cacctcgatc
     1741 tcacgcacct tcccgcggag acgattgaaa cgcgcttacc ggatatcacc gactttgtgc
     1801 gcacctacct cggtgtcgat cccgtatcgc agccgatccc ggtgcagccc accgcgcact
     1861 atgtgatggg cggcatcccc acggatctcg acgcgcgcgt cgtcatcgac gagaagaaca
     1921 cgcccctgcc ggggctgtac gctgccggcg agtgcgcctg tgtctcggtg catggcgcga
     1981 accgcctggg caccaactcg ctggtggaca ttcttgtctt tggccggcgt gccggcaagc
     2041 acgctgccag ctacgtgcgc gaggtggagc tgccaccgct gccacgcgat ccccaggcaa
     2101 gggcgcgcgc cgagatcgag cgcctcctgg cgaatactgg ggacgagcga ccgatcgcga
     2161 tccgcgacga gctgcgccgt gtgatgatgg aagacgctgg cgtggtgcgc acgggcgaga
     2221 gcctacagcg cgtgatcgag aagctcgggg agctgaagga gcgctatgcc cgcgtgggcg
     2281 tgcgtgacca gagcaagcag ttcaatacgg cgctcctgga gaccgtggag ctgggctatc
     2341 tgttggaatt ggcggaggtc ctggcgaaga gcgcgttcgc ccgagaggag agccgggggg
     2401 cgcactaccg cgaggatttc ccgaaccgga atgacac
//
LOCUS       DAOSCV010000695         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28262_length_2437_cov_2.686818, whole genome shotgun sequence.
ACCESSION   DAOSCV010000695 DAOSCV010000000
VERSION     DAOSCV010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28262_length_2437_cov_2.686818"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PLY56_15085"
     CDS             complement(<1..819)
                     /locus_tag="PLY56_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22851.1"
                     /translation="MAYAIQTEGLTKEFVSVSSARVTVALKDLTLQVEEGEIFGFLGP
                     NGAGKTTTIKILLGLIFPTAGRAWVLGKDIWDTAVKYDLAFVPDSPYFYDFLTAEELL
                     RFYGGLFGLKGSILDKRIEELLELVGMTHARGRRLREYSRGMFQRVGIAQALVNDPKV
                     LLLDEPTSGLDPIAQREVRDIILKLRERGKTVLLCSHHLSDVERISDRVAILNRGKLE
                     RMGRLDDLLAAERVEIAAEGLSEEATRKITDLAEESKVIAGRVTAYDARRDVINA"
     gene            1414..>2437
                     /locus_tag="PLY56_15090"
     CDS             1414..>2437
                     /locus_tag="PLY56_15090"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ22852.1"
                     /translation="MRRKVIRWWFLTAALLGALSAPGMANGVGDIEAIPEAGVRVTEV
                     EVRGNRQVSRDAILAVVTARPGRMITRDDLMRDVVRLRNLGYFQNVGTPEVEITPSGA
                     RVTYVVEEYPRIAGIQFEGNTVLTDAELEEIVGVERGQLLNVEALSARLRAIENSYRE
                     RGYLAQVGDVRVTPEGMVTIPIREVRVAAVEIEGVRRVRPQVVERALGLKDGDLFNEQ
                     QLQRDFVRLQQFGIFEAIEPEVGFNAAGDALVTWNLREGRSRALNFGLSYSPQESLVG
                     SIAFTENNFRGRAERLQVLLNIGSIGGRVGGEVAYATPLLGENIAYNARLFSTVEYRF
                     TQRLLIS"
BASE COUNT          423 a          755 c          814 g          445 t
ORIGIN      
        1 cgcgttgatc acgtcgcggc gcgcatcata agccgtcacc cggccggcaa tcaccttcga
       61 ttcctccgcc agatccgtga tcttgcgggt tgcctcttcc gagagccctt ctgcggcgat
      121 ctcgacgcgc tccgcggcaa ggaggtcgtc tagccggccc atccgctcca gcttgccgcg
      181 gttgagaatc gcgacgcggt cgctgatgcg ctccacatcg gagaggtggt gcgagcagag
      241 gagcaccgtc ttcccgcgct cgcgcagctt gaggatgatg tcgcgcacct cgcgctgggc
      301 aatcgggtcg aggccggaag tgggctcgtc cagcagaagc accttgggat cgttcaccag
      361 cgcctgagca atgcccacgc gctggaacat gccgcgggag tattcgcgca ggcggcgccc
      421 ccgggcatgc gtcatgccga ccagctccag gagctcctcg atccgcttat cgaggatact
      481 tcccttgagg ccgaagaggc caccgtagaa ccggaggagc tcctcggcgg tgaggaaatc
      541 gtagaagtag gggctgtcag gcacaaaggc gaggtcatac ttgaccgccg tatcccagat
      601 atccttgccg aggacccagg cccggccggc agtgggaaag atcagcccca agagaatctt
      661 gatggtggtg gtcttgccgg ccccgttggg gccgaggaat ccgaagatct cgccttcctc
      721 cacctggaga gtgagatcct tgagggccac agtcacgcgt gcgctggaga cgctcacgaa
      781 ctctttggtg aggccctccg tctggatggc atatgccatg cactccctcc tccctgttcc
      841 cacgggggaa ccggatggcg tccatgttac ctatcggaac caccccggcg tgcgccccga
      901 cctggacagc tactgccgcc gcatttatgt gccggtgcga agagcagatc ccctcgggcc
      961 cgctggcgac cggattgaaa ccgcgactag gcgcgcggat tggtctccag cgtgtatggg
     1021 gtgctcatgg cggtcgcggt tgttcgctta tgcaagcact tcccggcgct cgcgcaaagc
     1081 aagccggcag cctctcactt gcccattata acatagcaca atccgcgaga tcaacggcaa
     1141 tcccccgcgt ctcttgcccg aagagcgtcg ttcgccctgg ctggccaggc ccaaggggca
     1201 tcgcgcgccc atccgaccga ccgttcgccg aagcaggcgc gccgtgtttg cccccatgcc
     1261 cggtggggta agtataatcg gctcaagcag cgggaaagac ccggcgcttc cggcggggaa
     1321 gcacccggaa agctgcgcgt tctcgcctcg ggcattgcga cgcctactct tctgtgaccc
     1381 gcgagagcgc gtactcgttc caagggagtg cgcatgcgca ggaaagttat tcgttggtgg
     1441 tttctcacgg cggcgttgct gggggcactg agcgctccag gcatggccaa tggcgtcggg
     1501 gatatcgagg ccattccgga ggcgggcgta cgggtgaccg aggtcgaggt gcggggcaac
     1561 cggcaggtca gccgggatgc tatcctggcc gtggtgaccg cccgtccggg gcggatgatc
     1621 acccgcgacg atctgatgcg agatgtagtg cgcttgcgca acctcggcta cttccaaaac
     1681 gtgggcactc ccgaggtgga gatcaccccg tcgggagccc gcgtcaccta cgtcgtggag
     1741 gagtaccctc ggattgccgg cattcagttt gaaggcaata cggtgctcac cgacgcggag
     1801 cttgaagaga tcgtcggggt ggagcggggc caactcctga atgtggaggc gctgtcggcc
     1861 cggctgcgcg ccatcgaaaa cagctaccgc gagcgtggtt acctggccca ggtgggggat
     1921 gttagggtca cccccgaggg aatggtcacc atcccgatcc gggaggtgcg tgttgcagcg
     1981 gtggagatcg agggcgtgcg tcgcgttcgc ccgcaggtgg tagagcgcgc gctgggcctg
     2041 aaggatggcg atcttttcaa cgagcagcag ctccagagag acttcgtgcg cctgcagcaa
     2101 ttcggcatct tcgaggcgat cgagccggaa gtgggcttca acgccgccgg cgacgccctg
     2161 gtgacctgga atctgcggga gggccggtcg cgcgcgctca attttgggct gagctatagc
     2221 ccccaggaga gcctggtcgg gagcatcgcc tttaccgaga acaactttcg gggtcgggca
     2281 gagcggcttc aggtgttgct caacatcggt tcgattggtg gccgggtcgg cggggaggtg
     2341 gcctatgcca ccccgctcct gggcgagaat attgcctaca acgcgcgtct tttcagcacg
     2401 gtggagtatc ggttcaccca gcggcttctc atcagcc
//
LOCUS       DAOSCV010000696         2435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28287_length_2435_cov_2.836134, whole genome shotgun sequence.
ACCESSION   DAOSCV010000696 DAOSCV010000000
VERSION     DAOSCV010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28287_length_2435_cov_2.836134"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..716)
                     /locus_tag="PLY56_15095"
     CDS             complement(48..716)
                     /locus_tag="PLY56_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22853.1"
                     /translation="MRNNRRAFTLIELLVVIAIIAILAAILFPVFARARENARKAKCQ
                     SNLKQLAMAGLQYAQDYDECLFKCYQPSHLGASTLRWYYTSDTNVGLLYPYIKNAEVF
                     SCPTQNYYGANRALALGGGTGIITMASIEQPAATVWLGDALPKGGDPNTGMGPATTQK
                     GSELRPLTAANLASYGPGTACDARSLLAIRHSGMANIAFLDGHVKAMKSEATTSPEDM
                     WDLK"
     gene            complement(917..2164)
                     /gene="tyrS"
                     /locus_tag="PLY56_15100"
     CDS             complement(917..2164)
                     /gene="tyrS"
                     /locus_tag="PLY56_15100"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ22854.1"
                     /translation="MSNVFQKLRERGYIAQTSDDKAVEELLSGRTTLYEGFDPTGSSL
                     HIGHLLSLMAMHHLQQAGHRIIFLLGGFTAIVGDPTGKSETRQFITPAQVAANGEAMR
                     RQVQRLGLLDFESGDNPAVVRNNYDWLDMRLSDYMLNAARYFSINRLITHETYQERLK
                     RQEHLSLMEFLYSTLQGYDYLYLYKNEGCRLQIGGNDQWRNILDGVELIQYVLGEQAY
                     AMTFPLLLDPSGQKMGKTSTGMRVWLDPERTSPFEYYQYWVNTPDQDLERNLKLFTFL
                     PLDEIASILSGHPRDAQHRLAYEATKIAHGEEAARQAQSDSKSAFAAQGALPADVPTL
                     TLTEAELAEGVKVTNALADSGLVKSRGEARRLLQQGGIRLWDERLTDVDRIFTPEDFR
                     EVEGVRAIILRAGKGKVLKILLS"
BASE COUNT          450 a          721 c          791 g          473 t
ORIGIN      
        1 ccgaagcgat gcggatgtgc cacgggcgcg ggcccgagtg catccgacta tttgagatcc
       61 cacatatctt ccggcgaggt ggttgcctca gacttcattg ccttgacgtg tccgtctagg
      121 aacgcgatat tggccatgcc gctgtggcgt atggccagca gcgagcgggc gtcacacgcg
      181 gtgccagggc catacgatgc gaggttggcg gccgtgagag ggcgcagctc gctgcccttt
      241 tgggtggtgg cggggcccat gcccgtgttc ggatccccgc ccttggggag cgcgtcgccc
      301 agccagaccg tcgcggccgg ttgttcgata gaggccatcg tgatgatgcc ggtgccgcct
      361 ccaagcgcca gggctctgtt ggcgccgtag tagttctggg ttgggcagga aaacacttcg
      421 gcattcttga tgtaggggta gagcaggccg acgttggtgt cactggtgta gtaccagcgc
      481 agcgtggacg cgcccaggtg gcttggctgg tagcacttga agaggcactc gtcgtaatcc
      541 tgggcgtact gcagtcccgc catcgcgagt tgcttcaagt tggattggca tttcgccttg
      601 cgagcgttct cccgtgctct cgcgaagacc gggaagagga tcgcggcgag gatggctatg
      661 atggcaatga ccactagtag ctcaatcaac gtaaaggcgc gcctattgtt ccgcatagca
      721 atctctctcc ctccttggag ctgccgtcat ggacagcgcc atcggctcca tagagcgagg
      781 gcatcgagcg ggaacagact gcccgggccc tcaagtgccg cgcccattat acccgtgtat
      841 ccacgtggcg tcaacatctt tgccacaggg gcgttgagca ggggccggac agagaggccg
      901 cccggcccca aatggctcac gagagcaaga tcttgagcac tttgcccttg ccggcgcgca
      961 ggatgatggc acgcacgccc tccacctcgc ggaaatcctc gggggtaaag atgcgatcca
     1021 catcggtgag gcgctcatcc cagaggcgta tgccgccctg ctgaaggagg cgccgggctt
     1081 cgccgcgcga cttcaccaga ccgctatcgg ccagggcgtt ggtcactttc acgccctcgg
     1141 caagctcggc ctcggtgaga gtgagcgttg gcacatccgc tggaagcgcc ccttgtgcgg
     1201 caaaggcaga tttcgagtcg ctctgggcct ggcgtgccgc ttcctcgcca tgcgcgatct
     1261 tcgtggcttc ataggccagg cgatgctggg cgtcgcgcgg atgccccgag agaatgctgg
     1321 cgatctcatc gagcggcagg aaggtgaaga gcttcaggtt gcgctccagg tcctggtcgg
     1381 gcgtgttgac ccaatactgg tagtattcga agggcgaggt gcgctctgga tccagccaca
     1441 cccgcatgcc ggtcgaagtc ttccccatct tctggccgct gggatcgagg agcagcggga
     1501 aggtcatcgc ataggcctgc tcaccgagga cgtactggat cagttcgacg ccatcgagga
     1561 tgttgcgcca ctggtcattt ccaccgattt gaagccggca cccctcattc ttgtaaagat
     1621 agaggtagtc atagccctgc aaggtggagt agaggaactc catcagcgag aggtgctctt
     1681 gcctcttcag gcgctcctga taggtctcgt gagtgatcag ccggttgatg ctgaagtagc
     1741 gggcggcgtt cagcatgtaa tcggaaagcc gcatgtcgag ccagtcgtag ttgttgcgca
     1801 ccacggcggg gttgtcgccg ctctcaaaat cgagcagccc gaggcgctgg acctgccggc
     1861 gcatcgcctc gccgttggca gccacctgcg ccggcgtgat gaactggcgc gtttccgatt
     1921 tcccggtggg gtctcccacg atcgccgtga acccgcccag caagaagatg atccgatgcc
     1981 ccgcctgctg caggtgatgc atcgccatca aagagaggag atgcccgata tgcagggacg
     2041 agccggtagg gtcaaagccc tcatagaggg tggtccgccc tgaaagaagc tcttcaaccg
     2101 ccttgtcatc ggatgtttgg gcaatgtagc ctcgctcacg caatttctga aagacgttgc
     2161 tcacaatacg ctctccaacc aacgggaagc agtttcccgc atcgaaggga ccgatgctcg
     2221 cggggccgcg atcgaggaaa acagttgttg cgcagtcgag aggctccggt agctcccgtg
     2281 gcctcgctcc gcgagccagc agccacattg tagcatgggc gggatgcaat ggtcaacgcg
     2341 catggcgcgc tgggattctg acgtaaggaa tccgaagcca cggaggcagc gcgcaacccg
     2401 gcgcgcggcg ataaatcacc gcgctgcttg cgagc
//
LOCUS       DAOSCV010000697         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28295_length_2434_cov_3.844052, whole genome shotgun sequence.
ACCESSION   DAOSCV010000697 DAOSCV010000000
VERSION     DAOSCV010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28295_length_2434_cov_3.844052"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2434)
                     /locus_tag="PLY56_15105"
     CDS             complement(<1..>2434)
                     /locus_tag="PLY56_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22855.1"
                     /translation="VRVVTDASWRVSREGPEGWNRNGFDDSAWVAAREIGDAFMSPWY
                     ALGYGAEACATPAERQAREERILREEQEEKAALAFLEKTPRSRAKVAYQRGWPVLEVD
                     GRDVPPVLYSPRDLDPNTPQGAALVRQFRDAGIHLYHVVARLDAVWKAPGEYDFAPWD
                     QLLRKILIVDPEARILLGLRLDAPRWWLDAHKEEWVGYATGPAERGQDQIARFEAASM
                     ASETWMRDTGEAVRVALRHLEGVPWGRRIIGYQPNYGVYCEWHYYGMARDMPDTGPAM
                     TRRFRAWLRETYRNEGALRIAWKDPGARFEDARVPGREERLRAARLIFRDPGGTDRRV
                     IDYYRCHQRVVADCLLAYCRIVKEETKGRALTGAWYGYHFGMGYPPEGWHILLGEILQ
                     SPLVDFLTSPYGYHTQARAMGGDGQIRTVMESLRLHGKLHLYEADTRTHLADDRIIQA
                     RSLEETIAAIRREAGHALIRGSGLWWVDFGAGRNKGWFDHPEVLAEIDRLRALGAQAK
                     EWDGTSVSQVALVCDPESMYYLGYPPTLGYRLITGVYTELFRVGAPFDTILLDDLERP
                     GLPDYRVYIFLNCFYLDDAERERITRVVRQKGRTAVWLYGNGFLTPRGATTEGLEALT
                     GLTMEMVPVQTRMVAEITEPRHPLAARLPRSTRSTVKVQVEEPLPGALAAASWVNPRS
                     EQTMAKEYEHHSLRKEEDAIVWRFRGKGPSWTDIHCTAPLPACDALGLRVRTLRGPFS
                     FRIDLVDARGIPWSGPRVVVERSAWQTLSFPLADFNLASYAQERAEKPQFPIRAIKLV
                     ADLQPGTDYALAI"
BASE COUNT          399 a          844 c          803 g          388 t
ORIGIN      
        1 gatggccagg gcgtagtccg tgcccggctg gaggtcggcg accagcttga tggcgcggat
       61 cgggaattga ggcttctcgg cgcgctcctg agcataggat gcgaggttga aatcggccag
      121 cgggaaggag agcgtctgcc acgccgagcg ctcgaccacc actcgcgggc cgctccaggg
      181 aatcccgcgg gcatccacca gatcgattcg gaaggagaag gggcctctca gggtgcgcac
      241 gcgcagacca agggcatcac acgccgggag cggcgccgtg cagtggatat cggtccacga
      301 cgggcccttt ccgcggaagc gccacacgat ggcgtcttcc tccttgcgga gcgagtggtg
      361 ctcatactcc ttcgccatcg tctgctccga gcgcgggttt acccagctcg ccgcggccag
      421 cgcgccggga agcggctcct cgacctgcac cttcaccgtg ctccgtgtgc ttcggggcag
      481 cctggccgcc agcgggtggc gcggttccgt gatctcggcc accatccgcg tctggaccgg
      541 caccatctcc atcgtcaagc ccgtcagcgc ttcgaggccc tctgtcgtgg cgccgcgtgg
      601 ggtgaggaag ccattgccat acagccacac cgccgtccgg cccttttgcc ggaccacccg
      661 cgtaatccgc tcgcgctcgg catcatccag gtagaagcag ttaaggaaga tgtagacgcg
      721 atagtcggga agcccggggc gctccagatc atccaacagg atcgtatcga agggggcacc
      781 cacgcggaac agctccgtgt agacgccggt gatcagccgg tagcccagag tgggcggata
      841 ccccaggtag tacatgctct ctgggtcgca gacgagcgcg acctggctga cggaggtgcc
      901 gtcccactcc ttggcctggg cacccagcgc gcgcaggcgg tcaatctctg ccagcacctc
      961 gggatggtcg aaccaccctt tattgcgccc ggcgccgaag tcgacccacc agagcccgct
     1021 cccacggatg agggcatgcc ctgcctcgcg ccggatcgcc gcgatcgtct cttcgagcga
     1081 gcgcgcctgg atgatccggt catcggccag atgggtgcgc gtgtccgcct cgtagagatg
     1141 cagcttgcca tgaagccgca ggctttccat caccgtgcgg atctggccgt cgccccccat
     1201 cgcgcgcgcc tgcgtgtgat agccgtaggg cgaggtgagg aaatcgacga gtggcgattg
     1261 caggatctcg ccgaggagga tgtgccaccc ctctggggga tagcccatgc cgaagtgata
     1321 gccgtaccag gcgccggtga gcgcgcgccc tttcgtctcc tctttcacga tgcggcagta
     1381 ggcgaggagg caatcggcca ccacgcgctg gtgacaccgg tagtaatcga tgactcgccg
     1441 gtcggtgccg cccgggtcgc ggaagatgag gcgcgcggcg cggaggcgct cttcgcggcc
     1501 aggcacccga gcgtcctcaa agcgcgcgcc cggatccttc caggcgatgc gcaacgcacc
     1561 ctcgttcctg taggtctcgc gcagccaggc gcgaaaccgg cgggtcattg ccgggcctgt
     1621 atcgggcata tcccgggcca tgccgtagta atgccactcg caatagacgc cgtagttggg
     1681 ctgatagcca atgatccgcc ggccccaggg caccccttcc aggtggcgca gcgccacgcg
     1741 caccgcctcg cccgtgtcgc gcatccacgt ttcggaagcc atcgaggcgg cctcgaagcg
     1801 cgcgatctgg tcctggcctc tctccgccgg gccggtggca tagcccaccc actcctcctt
     1861 atgggcgtcg agccaccatc gaggggcatc cagacggagc cccaggagga tgcgcgcctc
     1921 cgggtctaca atgaggatct tccggaggag ctggtcccag ggcgcaaaat cgtactctcc
     1981 gggcgctttc cagacggcat cgagccgggc aaccacgtgg taaagatgga tgcccgcgtc
     2041 gcggaactgg cggacgagcg ccgcaccctg gggcgtgttc gggtcgagat cacgcgggct
     2101 gtagagcacg ggtggcacat cccggccatc cacctccaga acgggccagc cgcgctggta
     2161 agccaccttc gcccgcgaac gcggcgtctt ctccaggaag gccagggccg ccttctcctc
     2221 ctgctcctcg cgaaggatcc gctcctcgcg cgcctgccgc tcggcgggag tggcgcacgc
     2281 ctcggcgccg tagcccaggg cataccacgg tgacatgaaa gcgtcgccga tctctctggc
     2341 ggccacccag gcggagtcgt caaacccgtt ccggttccac ccttccgggc cctcgcgaga
     2401 gacccgccag ctggcatcgg tgacgacgcg cacc
//
LOCUS       DAOSCV010000698         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28313_length_2433_cov_2.904121, whole genome shotgun sequence.
ACCESSION   DAOSCV010000698 DAOSCV010000000
VERSION     DAOSCV010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28313_length_2433_cov_2.904121"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLY56_15110"
     CDS             complement(<1..322)
                     /locus_tag="PLY56_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22856.1"
                     /translation="MRDLSIIWRMMLLLAACGALHAAPAAETQPWQKLYTGQDATGPN
                     VIALWQFQPGQEAKDASGHGHDLKLRGNTRFIAGGPLGGYLESFLGGTDNDTQMGAVA
                     KDHPD"
     gene            complement(491..1489)
                     /locus_tag="PLY56_15115"
     CDS             complement(491..1489)
                     /locus_tag="PLY56_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ22857.1"
                     /translation="MKSRHVWILAWAAVVALAPAADAAEKASPLASRRAALVALAAKG
                     PAGIPQIAAALKDDNLVIRRTAVRLLADLGKPARPALAEALSNSDFLVRRTALTALCE
                     PLTAECLPHLEKSIQDESLAVRLVAVNLLASLEPRSDAVNKILDKARSDESTAIRDVA
                     ARALWPFHRDTVLLRDRKDWDHEVVVTQSIPLPKTGWRFQRDPERDGHLKKWFAPDLD
                     DSKWPAIEIERSWESQGHEYDGVAWYRGSFELPAKPDHNAVELHFDAVDECAWVWVNG
                     IYVGQHDLGTAGWDRPFAIDITREVRWGQKNQVTIRVYDSAYAGGIWKPVRIDILK"
     gene            complement(1658..>2433)
                     /locus_tag="PLY56_15120"
     CDS             complement(1658..>2433)
                     /locus_tag="PLY56_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ22858.1"
                     /translation="KTALTAAQTEFLLKTLDDVEKFLSLPYTPPAIDLMRFHQLRADV
                     GEEGLVLLGIPNAICLPATLFSPQDLCLAWADAPDLVRRLCAVASERLLDYTQRLLDG
                     GVDAFRIIGGEYASVQLGPQAFRKLVTPFDTELVALMHSRGAVAYYHNHGQVMEYLDL
                     LADLGVDALDPLEAPPWGDVDLRAAMARIGDRVCLVGNLDDMEIIDRLPEAEVCAIAR
                     ERLEAAGSHHFILGGTASGTCGEHGARNFIAMARMVRAG"
BASE COUNT          421 a          803 c          788 g          421 t
ORIGIN      
        1 gatccgggtg atctttcgct acggcgccca tctgggtgtc gttgtcggtg ccaccaagaa
       61 acgactcgag gtagccgccc agcggcccgc cggcaatgaa gcgggtgttg ccccggagct
      121 tcaagtcgtg gccgtggccg gaagcatcct tggcctcttg cccaggctgg aactgccaga
      181 gggcgatgac gttcggtcca gtggcatcct ggccggtgta gagcttctgc cagggctggg
      241 tttcagcggc tggcgcggcg tggagcgccc cgcatgccgc aagcagcaac atcatgcgcc
      301 agatgatgga tagatccctc atgactgcac cattctctcc aggagcgcgc gacaggtcgt
      361 cggaagcttc gccccgcggg tatctcgagg caggtcaacc ggcaataccc aggttggacg
      421 ccaccccgat gccctcgtga gggggttagc gcgcgctcgt ccgaatacgg atcccgttcg
      481 gcggaccgct ctacttcaag atatcgatac ggaccggctt ccagatcccg cccgcatagg
      541 cgctgtcgta gacgcgaatc gtgacctgat tcttctgccc ccagcgcacc tcgcgggtga
      601 tatcgatggc gaagggccga tcccatccgg cagtgcccag gtcatgctgg ccaacgtaga
      661 ttccgttcac ccagacccag gcgcactcat ccacggcatc gaagtgcagc tccaccgcgt
      721 tgtggtccgg ctttgctggc agctcgaaag agccgcgata ccacgccacg ccgtcatact
      781 catgcccctg gctctcccag gagcgctcga tctcgatggc cggccacttg gagtcatcga
      841 gatccggggc gaaccacttc ttcaggtgcc cgtcgcgctc gggatctcgc tggaagcgcc
      901 agccggtctt cgggagtggg atcgactgtg tcacgaccac ctcgtggtcc cagtccttgc
      961 ggtcgcgcag gaggaccgtg tcacggtgga agggccacag cgcccgtgcg gccacgtcgc
     1021 ggatcgcggt ggactcatcc gagcgcgcct tgtcgaggat cttgttcacc gcatcgctcc
     1081 gaggctcgag cgaggccagg aggttcacgg ctaccaggcg cacggccagg ctttcgtctt
     1141 gaatgctctt ctccaggtgg gggagacact ccgccgtgag cggctcgcag agcgcggtga
     1201 gcgccgttcg ccgcacgagg aaatcagagt tggacaacgc ctcggcaagt gccggccggg
     1261 ccggctttcc cagatcggcc aggaggcgga ctgccgtgcg ccggatcacc aggttgtcat
     1321 ctttgagcgc agcggcgatc tgtggaatgc ccgccggccc ctttgccgcg agggcaacga
     1381 gcgcggcgcg gcgcgatgcc aacggtgagg ccttttctgc cgcatcggcg gctggcgcta
     1441 acgcgaccac cgccgcccac gcaaggatcc aaacgtgtct gctcttcacc ggttctcctc
     1501 catcatcacg aaggtgttac ctactttgcg cctcattcga cagtcgaacg ttgcgactcc
     1561 tgctcaggag ggaggccggc cactcgccct gctcgccagc cattgccctc caagttgcgc
     1621 gcctctcgtg cgagcgccgc atccgcggcc cgggaagcta cccggcgcgg accatccggg
     1681 ccatggcaat gaagttgcgc gcgccatgtt cgccacaagt gccagatgcc gtgccgccca
     1741 agatgaagtg gtgggagccg gcagcctcca gtcgttcgcg ggcgatggcg cacacctctg
     1801 cctcgggcaa gcggtcgatg atctccatgt catccaggtt gcccacgagg cagacgcggt
     1861 caccaatgcg agccatcgcc gcgcgcagat ccacgtcgcc ccaaggtggt gcctccaggg
     1921 gatcgagggc atccacgccc agatcagcga gcagatccag atactccatc acctgcccgt
     1981 ggttgtggta gtaggccacg gcgccgcggg agtgcatgag cgccacgagc tcggtgtcga
     2041 agggggtcac cagtttccgg aacgcctggg gacccagctg cacgctcgcg tactcgccgc
     2101 cgatgatccg gaaggcatcc acacctccgt ctagcaggcg ctgagtgtaa tccagaaggc
     2161 gttcggaggc aaccgcgcac agccttcgca ccagatccgg cgcatcagcc catgccaggc
     2221 acaggtcctg gggcgagaag agcgtcgcgg gcaggcagat cgcgttcggg atgccgagca
     2281 ggacgaggcc ttcctcccca acatcggcgc gcagttggtg gaagcgcatc aggtcgatgg
     2341 cgggcggggt gtagggaagt gagaggaact tctcaacatc atccagcgtc ttgaggagaa
     2401 actcggtctg cgctgcggtg agggcagtct tcc
//
LOCUS       DAOSCV010000699         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28376_length_2429_cov_3.863100, whole genome shotgun sequence.
ACCESSION   DAOSCV010000699 DAOSCV010000000
VERSION     DAOSCV010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28376_length_2429_cov_3.863100"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLY56_15125"
     CDS             <1..917
                     /locus_tag="PLY56_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22859.1"
                     /translation="ESAGLLEVVEPKHGLDSIGGLEYIKERLMKIARYMREGDTKRVP
                     MALAFGGPPGTGKTAVAFAFAKESGVNCVILKEAREMWVGSSERNQEYVFRLIEAMAP
                     VIVIVDEADQSEGSRGNHTDSEVDKRLWAQKIRFMGDPAHRGRIIFIFTSNRLDLIDV
                     AMKRRGRIDQIIPFLYLEDKMRPMVFQAIFKKQGIPYDVSDWQPIIDATDRFSGADIE
                     SIVLSADELAAEEKAPAVTQQHLLAALDDFIQARNEEMIRYMELLALTECSARSMLPE
                     SLRETFDRNAALEELDQIRAKLKLEGVI"
     gene            1016..1951
                     /gene="rnz"
                     /locus_tag="PLY56_15130"
     CDS             1016..1951
                     /gene="rnz"
                     /locus_tag="PLY56_15130"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOJ22860.1"
                     /translation="MTGRHAARHARITFLGTSSGAPTLARGLPSLALRRGPELFLFDC
                     GEGTQLQFRKAGLRPGRLSRVFISHLHGDHVLGLPGFLMSLQLGGRATPLEIHGPPGI
                     AALIQAVLRLVEAHIGYELRLFEHGAAETVARGDGYQIECRPLDHRAFCLGYALVEAP
                     VPGALDVARARALGVPDGPMMGRLKRGESVTLSSGATVEPTQVVAPARRGLRVAYCTD
                     TRPSDEAIALARDADLLIHEATFAHDRAEDAHAKGHCTAREAAEIATRAGARQLALTH
                     ISPRYGDPAPLLSEAQAVFPNTVIAADLMALEVTA"
     gene            complement(2128..>2429)
                     /locus_tag="PLY56_15135"
     CDS             complement(2128..>2429)
                     /locus_tag="PLY56_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22861.1"
                     /translation="SLFLGGWVAARLASATTTASGAWHGVIVWALALVLGTLLTAIGV
                     TGVLGYLPSRVLTTPGVTPTDIASNAWGFFIGALLGLAAAAWGGSVGKRREGTEA"
BASE COUNT          410 a          815 c          797 g          407 t
ORIGIN      
        1 atgagtcggc gggcctcctg gaggtggtgg agccgaagca cggcctggac agcatcggcg
       61 gcctggagta tatcaaggag cgcctgatga agatcgcccg ctacatgcgc gagggcgata
      121 ccaagcgcgt gccaatggcg ctggctttcg gcggcccccc cggaacgggc aagacggcgg
      181 tggccttcgc tttcgccaag gaatcgggcg tcaactgcgt gatcctcaag gaagcacgcg
      241 aaatgtgggt gggctccagc gagcgcaatc aggaatacgt cttccgcctg atcgaggcga
      301 tggcccccgt catcgtcatc gtggatgaag cagaccaatc cgagggctcg cgcggcaacc
      361 acaccgactc cgaggtggat aagcggctct gggcgcagaa aatccgcttt atgggcgatc
      421 ccgctcaccg cggccggatc atcttcatct tcacgagcaa ccgcctggat ctgatcgacg
      481 tggcgatgaa gcgccgtggg cgaatcgacc agatcattcc tttcctttat ctggaagaca
      541 agatgcgccc gatggtcttt caggccatct ttaagaaaca aggcattccc tacgacgtct
      601 ccgattggca gccgatcatc gacgcgaccg atcggttttc cggagcggat atcgagtcga
      661 tcgtcctgag cgccgacgag ctcgctgccg aggagaaggc gccggcggtg acgcaacaac
      721 acctcctggc ggcgctggac gatttcatcc aggcgcgcaa cgaggagatg atccgctaca
      781 tggagctcct ggcgctcacc gaatgctccg cgcgcagcat gctcccggag tctttgcggg
      841 agacgttcga ccgcaacgct gccctggaag agctggatca gatccgcgcc aagctgaaac
      901 tggaaggcgt gatctgagaa caggcgcttg cgctctgctc ccggtcgttc tgctgcaatt
      961 tgaagaaggc ttccgtggcc gggccggcca cgcgggagaa gcaagggagg tgccgttgac
     1021 cggccgacac gccgcgcgac atgcccggat caccttcctg ggtaccagct ccggcgcgcc
     1081 caccctggcg cgcggcctgc cgtcgctcgc gctccggcgc ggccccgagt tgttcctctt
     1141 cgattgcggc gagggcaccc agcttcagtt tcgaaaagcg ggcctccgcc cggggcggct
     1201 ctcgcgcgtc ttcatcagcc atttacatgg cgaccatgtt ctgggcctgc ccgggtttct
     1261 catgagcctg caactcggcg ggcgagccac tcccctggag attcacgggc cacccggcat
     1321 cgcggcgctg attcaggcgg tcctgcgcct ggtcgaggca cacatcggct acgagctacg
     1381 tctctttgaa catggcgcgg cggagaccgt ggcgcgcggc gatggttatc agatcgagtg
     1441 tcggcccctg gaccatcgcg cgttctgcct tggctacgcg ctggtcgagg cgcctgttcc
     1501 gggggctctg gatgttgctc gcgcgcgcgc cctgggcgtg ccggacgggc cgatgatggg
     1561 ccggctgaag cggggcgagt cggtgaccct ttctagcggc gccaccgtgg agccgactca
     1621 ggtggtggcc ccggcccgtc gcggcctgcg cgtggcctat tgcaccgata cccggccctc
     1681 agatgaggcc attgcgctcg cccgggacgc ggatctcttg atccatgagg ccaccttcgc
     1741 gcacgaccgt gccgaggatg cccatgcgaa aggacattgc acggcgcgcg aggcggcgga
     1801 gatcgcgacg cgcgccggcg cgcgccagtt ggcgctcacg cacatcagcc cccgctatgg
     1861 cgatcccgct cccctgctat cggaagcgca ggccgtcttt ccaaacacgg tgatcgccgc
     1921 cgatctgatg gcactggagg tcacggcata gctcacgctg ccgccgacct cggaatctcg
     1981 aactctcccc ggccgcaatg gagagaggcg ggggctggtc cgggcgtcac ccggcgagga
     2041 gaacgggggc caacgtgccc ccgccatctt tcgcaggtgc cgcgcggcca gccagcccgc
     2101 ttctctctcg tgggtgtggc gcgctggcta ggcttcggtg ccctctcgtc gcttccccac
     2161 cgagccgccc caagccgctg cggccagccc aagcagagcc ccgatgaaga agccccacgc
     2221 gttgctcgcg atatcggtcg gggtcacccc cggtgtggtc agcactcgcg atggcaggta
     2281 gcccagcact ccggtgacgc caatggctgt cagcagcgtg ccgagaacca gcgcgagtgc
     2341 ccacacgatc acgccatgcc acgcgccgct cgcagtcgtc gttgccgaag ccagtcgtgc
     2401 agccacccag ccgccaagga agagcgaga
//
LOCUS       DAOSCV010000700         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28406_length_2427_cov_3.654722, whole genome shotgun sequence.
ACCESSION   DAOSCV010000700 DAOSCV010000000
VERSION     DAOSCV010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28406_length_2427_cov_3.654722"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLY56_15140"
     CDS             complement(<1..477)
                     /locus_tag="PLY56_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOJ22862.1"
                     /translation="MPMDTGEVVAVTFQPLVSETALQITEKRDGEVAYVGATGEIDLH
                     GEEMLRRVLESSLSQGGYSVILDLRHVSYMDTGALKILLETKRRTLGCGGELYILVEE
                     SLPKQVIYMAHLQDAVKMYETVDQALEDIARRATCRGYFARSAARAEEPEGRETLLP"
     gene            complement(771..1454)
                     /locus_tag="PLY56_15145"
     CDS             complement(771..1454)
                     /locus_tag="PLY56_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HOJ22863.1"
                     /translation="MKRSEINRILRETIAFFDMHRFKLPPFAFWTPADWERRGEEADE
                     IRDNLLGWDITDFGLGDFQRKGLVLFTIRNGSHGDPRYPKPYAEKIMVAGENQETPMH
                     FHAFKMEDIINRGGGNLLIELYNATPDNDLDRTSPVVVSVDGVRRTLEPGATLRLTPG
                     ESITLTQRLYHRFYGEEGRGRVLIGEVSRVNDDRTDNYFHEPVGRFPAIEEDEPPLYY
                     LCTEYPRAK"
     gene            complement(1458..1652)
                     /locus_tag="PLY56_15150"
     CDS             complement(1458..1652)
                     /locus_tag="PLY56_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22864.1"
                     /translation="MEQVTASFTAISFERGWLRDGNACWLHRLIYRDDKASRSGIRLV
                     LACQKTLEALPLSARVLQRS"
     gene            complement(1853..1928)
                     /locus_tag="PLY56_15155"
     tRNA            complement(1853..1928)
                     /locus_tag="PLY56_15155"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1893..1895),aa:His,seq:gtg)
     gene            2275..>2427
                     /locus_tag="PLY56_15160"
     CDS             2275..>2427
                     /locus_tag="PLY56_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOJ22865.1"
                     /translation="MKVSIIGGAGRVGSNAAYALQLVGWAREIALVDVMTEQAEGEAL
                     DLRHGAS"
BASE COUNT          433 a          760 c          805 g          429 t
ORIGIN      
        1 aggaagcaac gtctcccgcc cctcgggctc ctcggctcgc gcggcactgc gcgcgaagta
       61 tcccctgcaa gtcgcccggc gcgcgatatc ctccagcgcc tggtcaaccg tctcgtacat
      121 cttcacggcg tcctgaagat gcgccatgta gatcacctgc ttgggaaggg attcttccac
      181 gaggatatag agttcaccgc cacagccaag ggtgcggcgc ttggtttcga ggaggatctt
      241 cagcgcgccc gtatccatgt agctgacgtg gcgcaggtcg aggattacgg aatatccccc
      301 ctgagagagg gagctttcca ggacgcggcg cagcatctcc tcgccatgga ggtcaatctc
      361 gcccgtggcg ccgacgtagg ccacctcgcc atccctcttc tccgtgattt gcagcgcggt
      421 ctcgctgacc agcggctgaa aggtcacggc caccacctcc ccggtatcca ttggcatccg
      481 cgcttcctcg cattcgcttc acccgtaccg ctcagggagt cgcaactggc cggccacacg
      541 acgcgcatca cggaacagcc cgagggcggc tctcgctgca gcagatggcg cggcgatggc
      601 atctctggaa cggcccacct gggccaggcg caacccgcca gtacctttag attcctcgcc
      661 gcgccaccaa ctcctgccag atcgcggatg cgcggcccca tcggttcatc cgcggggccg
      721 ccggggaggc tcgccgcagc gcgagcttgc cgtccattcc cggaaggccg ctatttggcg
      781 cgcgggtatt cggtgcagag gtagtagaga ggaggctcat cctcctcgat ggcgggaaaa
      841 cgccccaccg gctcatggaa gtagttgtcg gtgcggtcgt cgttgacgcg cgacacctca
      901 ccgatgagga cgcggccccg gccctcttca ccatagaagc gatggtagag gcgctgggtg
      961 agggtgatgc tctcgcccgg ggtcaggcgc agggtggctc ccggctccag ggtgcgccgc
     1021 acgccatcca cggagaccac cacaggcgac gtgcgatcca ggtcgttgtc cggcgtcgcg
     1081 ttgtagagct cgatgagaag gttgcccccg ccgcggttga tgatatcttc catcttgaag
     1141 gcatggaagt gcatcggcgt ctcctggttc tcgccggcaa ccatgatctt ttccgcgtac
     1201 ggcttcggat agcgcgggtc gccgtggctc ccgttgcgga tggtgaagag aaccaggccc
     1261 ttgcgctgga agtcccccag gccgaagtcg gtgatgtccc agcccaggag gttatcgcgg
     1321 atttcatccg cttcctctcc tcgcctctcc caatctgcgg gggtccagaa ggcgaatggc
     1381 ggcagcttga agcggtgcat atcaaagaag gcgatggtct cgcgcaggat acggttgatc
     1441 tccgaacgct tcatggctca actcctttgc aggacgcgcg ccgagagggg aagagcttcc
     1501 aacgtcttct ggcaagccaa cacgaggcgg atgccagagc gggaggcttt gtcgtcgcga
     1561 tagatgaggc ggtgcagcca gcaggcgttg ccgtcgcgca gccatccgcg ctcgaaggag
     1621 atagccgtaa aggacgcggt gacttgctcc acgatctcgg ccggcgcctt cgcctgagcc
     1681 tcgggcagcc tcaggggaag ccgttatcgt cctgttcacg cggcatgata ccacacgcga
     1741 ggggaattgt gcaagcgtta cgacgaaggg gagagaggca ggggctaggt cccatccgct
     1801 tttcacgacg cggctttccc gcgccgctcc aagggacccg ccccctgcca aatggggtga
     1861 gtgaggggat tcgaaccccc gatctccaga tccacagtct ggcgccttag ccgctaggcg
     1921 acactcacca ccaacgcttt gcatgttacc atatgcaggg ctggtctgtc aagcgaaatt
     1981 cgccgctcgc gcagggggcg ccagatgatg gaatccgccg cagtgcaaag gctgcgggcg
     2041 gcctcgcctg gcatggccgg cttcaggcag cttccgacac ccaacaagga tggtggcgtc
     2101 tggcaagcga atgggatacc cgcgggcggt tggcacaggc ggccagggca gccccggggc
     2161 gagacagaaa cgctcgccca ggcgggtgag gcggatcccg tgagggcacg cgcccggcgg
     2221 tgcctgcgga gcagcctcag cgcggcgttt ccatggtata tagagaggac gcaaatgaag
     2281 gtcagtatta ttggcggagc agggcgagtg gggtcgaacg cggcctacgc tctgcagttg
     2341 gttggttggg cacgcgagat cgcgctggtg gacgtgatga cagagcaagc ggagggggag
     2401 gcgcttgatc tgcggcatgg ggcatcg
//
LOCUS       DAOSCV010000701         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28439_length_2425_cov_3.692827, whole genome shotgun sequence.
ACCESSION   DAOSCV010000701 DAOSCV010000000
VERSION     DAOSCV010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28439_length_2425_cov_3.692827"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="PLY56_15165"
     CDS             complement(<1..1110)
                     /locus_tag="PLY56_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22866.1"
                     /translation="MRSRILTGLWLLALCAGVAGPAIGEERAPDWIAREKIRAGWIYY
                     GDGADKLRFFKEHGLNALITSTANAETFDTWAKEAKRAGMRLFGVLGFSFDAEKAGMR
                     CAVFGNGYKSTVACPLNEEFWQQRMIEPAVRLARQGMDGEREISGILIDFELYQNTNK
                     GGQIYYTDACYCDYCFGEFLKSKALPDPGAVPFAQRVAWLKEKGVYEEYHPFLQTRVR
                     ALAEKMRDAVRAVHKEFFLGFYPIPHNWMLVGVAQGLGTPDHPMILWATATYGGGGAE
                     KIPDNWREEMEKQGIHAYYCGGMLLRMYSAANLAAHMTRVATKTNGYWLFTVHTLCIP
                     PEQQRGDYYLAAGTREDYLREIRRANAEIDRLCADP"
     gene            complement(1228..>2425)
                     /locus_tag="PLY56_15170"
     CDS             complement(1228..>2425)
                     /locus_tag="PLY56_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22867.1"
                     /translation="LKPGQYEGTLVVEGAGQPITVPLRVRVSKITMGRPRLSLGMWDY
                     TDGTGTYGLTLKNLGPAIALQQSHFVDTPWARRGVLPWPEASAFDAQHQLKQPLSFTS
                     FDEWVKRWPNARNYFVFASVSSSFAGAKIGTPEFAGRVGSWAKALAQHMRDLGMKPQQ
                     LGILLVDEPHSDAQDEIIAAWARAFKAAAPEITLFQDPTWERPDKTKIQEAITLCDIV
                     CPNIPIFYRGGPEVARYFEARRTAGQKMWFYQCAGPAKLFDPYRYHRLQSWHCFQHGA
                     VGMGFWAFGDTGKALSSWNEYAAAGTPYTPAFIGIDDVTDGVHWQAVREGIEDYEYLS
                     MLRDAAQKTKDAGLKAQAEALLAEAPRAVLGEFKSNYDWKVEADHTGADTYRLRVLAL
                     LEKMAQ"
BASE COUNT          390 a          807 c          794 g          434 t
ORIGIN      
        1 gggatcggcg cagaggcggt ctatttcggc gttcgcgcgc cggatctcgc gcaggtagtc
       61 ctcgcgcgtg ccggcggcca ggtagtaatc accccgctgc tgctccgggg ggatgcacag
      121 ggtgtgcacg gtgaagagcc agtagccgtt cgtcttcgtg gcgacgcgcg tcatgtgcgc
      181 tgccaggttg gccgccgagt acatgcgcag gagcattccg ccgcagtagt aggcgtggat
      241 cccttgcttc tccatctcct cgcgccagtt gtccggaatc ttctccgcgc cgccaccccc
      301 gtaggtcgcc gttgcccaca ggatcatcgg gtggtcggga gtgcccaggc cctgcgccac
      361 gccgacgagc atccagttgt gcgggatggg gtagaacccg aggaaaaatt ctttgtgcac
      421 ggcgcggacg gcgtcgcgca tcttctccgc gagggcgcgc acgcgcgtct ggaggaatgg
      481 gtgatactcc tcgtaaacgc ccttctcctt gagccacgcc acgcgctggg cgaagggaac
      541 ggcgccgggg tccgggagcg ccttcgattt caggaactcg ccgaagcagt agtcgcagta
      601 gcaggcgtcg gtgtagtaga tctggccgcc tttgttcgtg ttctgataga gctcgaaatc
      661 gatcaggatg ccggagatct cgcgctctcc atccatcccc tggcgagcga ggcggaccgc
      721 cggctcgatc atccgctgct gccagaactc ctcgttgaga gggcaggcga cggtgctctt
      781 gtagccgttg ccaaagacgg cgcagcgcat cccggccttc tccgcgtcga aggagaagcc
      841 gaggacgcca aagaggcgca tgccggcgcg cttcgcctcc ttcgcccacg tatcgaacgt
      901 ctcggcgttc gcggtgctcg tgatgagcgc gttcaacccg tgttctttga agaagcggag
      961 cttgtcggcg ccgtcgccat agtagatcca tccggcacgg atcttctcgc gcgcgatcca
     1021 atcgggcgcg cgctcttctc cgattgctgg cccggccact cccgcgcaga gcgcgagaag
     1081 ccagaggcca gtcagaatcc gcgatctcat cagcgttctc ccgatacccg gcgtcagccg
     1141 gcgggcgagt gccatccgga ctgccgggta acgcgagcgc ccggggcagg gcaccagcgc
     1201 ctgccccggg cgcctcctga aagagcgcta ctgcgccatc ttctcgagca atgccagcac
     1261 gcgcaggcga taggtatccg cgcccgtgtg gtcggcttcc actttccagt cgtagttcga
     1321 cttgaactcg cccagaacgg cacgcggcgc ctccgccagg agcgcctctg cctgagcctt
     1381 caggccggca tcctttgtct tctgcgcggc gtcgcggagc atcgagaggt actcatagtc
     1441 ctcgatcccc tcgcgcactg cctgccagtg aacgccatcc gtgacatcgt cgatgccgat
     1501 gaacgccggc gtgtagggcg tgccggcggc ggcatactcg ttccaggagc tgagtgcttt
     1561 cccggtatca ccgaaggccc agaagcccat gcccacggcg ccatgctgga agcagtgcca
     1621 gctttgcagg cggtggtagc gataggggtc gaacagctta gccggtccgg cgcactgata
     1681 gaaccacatc ttctgcccgg cggtgcggcg cgcctcgaag tagcgcgcga cttccggacc
     1741 gccccggtag aagatcggga tgttcgggca gacgatatcg cagagcgtga tcgcttcctg
     1801 gatcttcgtc ttgtcaggcc gctcccaggt gggatcctgg aagagggtga tctccggcgc
     1861 cgccgccttg aaggcgcgcg cccaggccgc gatgatctcg tcctgcgcgt cggaatgcgg
     1921 ctcgtccacc aggaggatgc cgagctgctg cggcttcatc ccgagatcgc gcatatgctg
     1981 tgccagcgcc ttcgcccacg agccaacgcg cccggcgaac tctggcgtgc cgatcttcgc
     2041 gcccgcgaag ctggagctca cgctcgcgaa gacgaagtag ttgcgcgcgt tgggccagcg
     2101 cttcacccac tcatcgaaac tcgtgaagga gagaggctgc ttgagttggt gctgcgcgtc
     2161 gaacgccgag gcctccggcc agggcagaac gccgcgccgg gcccacggcg tatccacgaa
     2221 gtgcgactgc tggagggcaa tcgccggacc gaggttcttg agcgtgaggc cgtacgtccc
     2281 ggtcccgtcg gtgtagtccc acatccccag cgagaggcgt ggccgtccca tcgtgatctt
     2341 cgagacgcgc acgcgcagcg gcaccgtgat cggctgcccg gcgccctcaa ctaccagcgt
     2401 gccctcatac tggcccggct tcagg
//
LOCUS       DAOSCV010000702         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28447_length_2425_cov_2.672996, whole genome shotgun sequence.
ACCESSION   DAOSCV010000702 DAOSCV010000000
VERSION     DAOSCV010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28447_length_2425_cov_2.672996"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLY56_15175"
     CDS             complement(<1..881)
                     /locus_tag="PLY56_15175"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ22868.1"
                     /translation="MTAVMLLGVVLCIARPGTALAAEAGAPSGGGKPATPAPAASGQG
                     PTAPAPTSRTSPLPENPQSPARQWLPAAPTSEGEPRPAADADQLKPFGMDFFAAAPAD
                     FTVAPDVPIPAGYRLGKHDKLRIRYWAPAVPEVTHEIEVGEDGMVVVPGIGDVNAHGL
                     TREEFRQRLGKRLREQLKNVSFSADLIETRTISVFVTGAARRPGRYTVKAESNLFNVI
                     YAAGGASPEGTLRKVALRRRNKLVVEVDLYQFLNSGTLNADVMLEDQDVVFFPVAGAR
                     VTVGGEVVRPAIYEVVPG"
     gene            1323..2132
                     /locus_tag="PLY56_15180"
     CDS             1323..2132
                     /locus_tag="PLY56_15180"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22869.1"
                     /translation="MGDLTATEMKAREPWREGDVLVVPLEGDLDLETAPATRERLLRL
                     LAQGHRNLIADLQHCSYVDSSGVLALLSVADRATELGGWLHLRHPSRTLCRVLFLAHV
                     AERLGVPHTAFPPKQPRSFLDTGVGQWEVARFVLPATPQTCAVARRIVLAEAARLPLA
                     KEDLADLELAVGEAFANAVRHGSPRGAEDRVTLRTLYSSEAFVVEITDRGRGFDRSQL
                     TLPSPEEIREGGMGILYMEQLMDAATFESGQWGTTVRLIKALPRPAERSCL"
     gene            complement(2144..>2425)
                     /locus_tag="PLY56_15185"
     CDS             complement(2144..>2425)
                     /locus_tag="PLY56_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22870.1"
                     /translation="RLDGLAVLLVLASVISLYYYLRVVRYMYMEAPAASGAEGKQEAL
                     SRSLSLRVAVLMAVALVVLLGLFPTAALTLAQTAGITLTEGGIPILRGG"
BASE COUNT          433 a          839 c          774 g          379 t
ORIGIN      
        1 ccgggcacca cctcgtagat agcagggcgg actacctctc ccccgacggt cacgcgcgct
       61 cccgcgacgg ggaagaagac cacatcctga tcctcgagca tgacgtccgc gttgagtgtt
      121 cccgagttga ggaactggta gagatccacc tcgacgacca gcttattgcg ccggcgcaac
      181 gccaccttgc gcagcgttcc ctcgggagaa gccccgccag cggcatagat gacgttgaac
      241 aggttggact ccgccttcac cgtgtagcgt ccgggccgcc gggccgcccc ggtgacgaag
      301 accgagatcg tccgcgtctc gatgagatcg gccgaaaagc tgacgttctt tagctgctcg
      361 cggaggcgct tgccgaggcg ctgccggaac tcctcgcggg tgagcccgtg ggcgttcacg
      421 tcgccgatcc ccggcactac caccatgcca tcctcgccca cttcaatctc gtgggtcact
      481 tcaggcaccg cgggcgccca gtagcgaatg cggagcttat catgcttccc caggcgatag
      541 ccggccggga tgggcacatc gggcgccacc gtgaaatcag cgggagctgc ggcaaagaag
      601 tccatgccaa acggcttcag ctggtcggca tccgccgcag gccgtggctc tccctcgctc
      661 gtcggagctg ccggaagcca ctgccgcgcc ggcgattgag ggttctccgg caaaggagag
      721 gtacgggaag taggtgccgg cgccgtcgga ccctgcccgc ttgcggccgg cgcaggcgtc
      781 gccggcttcc ctcctccaga cggcgccccg gcctcggccg ccagcgcggt gccagggcgc
      841 gcgatgcaaa gcaccacccc gagtagcatg acggcagtca tagcgagcgc cttgcagggg
      901 cgctgctgac tgctggccag ccgcccgaac caaccatgat cacagatgga ataactcagc
      961 aagcgcttcg atgaagaacg cctgatccgc atggaaagac caccccttcg gaaacaccga
     1021 ccaggagccc gcggttcatc cccctggcgg caggggaacc ctcgaagctc ccgaggcagg
     1081 tgacaagcgc cactcccaag cccgaaaggt cgctcgtctg cctcaaccta tctttggcag
     1141 tagagcgcgg gatctccttc ttcccgcgtg aaagacgcat ccgaggagtg gttcgaccgc
     1201 gtttccctgg ccttcacgcc cggcggcatg cccaggcacc gggagatcag gaagcgcatc
     1261 ctgaccccac atcgccattt gacgggcccg ctgaggagtg gtagaataac cgtggtgacc
     1321 ctatgggcga tctgacagca accgagatga aggcacgaga gccatggcgt gagggagacg
     1381 tcctggtcgt cccgctggaa ggtgacctgg acctggagac ggctcctgcc acgcgagagc
     1441 gtctcctgcg cctgctggca caaggccacc gaaatctgat tgctgacctc cagcactgct
     1501 cctatgtgga tagcagcggc gtcctggcac tgctatcagt cgccgaccgc gcgactgagt
     1561 tgggcggttg gctccacctg cgccatccat cgcgcacgct gtgccgcgtg ctgttcctgg
     1621 cccacgtcgc ggagcgcctg ggggtgcccc acacggcatt cccgccgaag caaccgcgct
     1681 cgttcctcga taccggcgta gggcaatggg aggtggcgcg cttcgtcctg ccggctacgc
     1741 cgcagacgtg cgcggtcgcc cggcgcatcg tcctcgccga agccgccaga ttacccctcg
     1801 cgaaggagga cctcgcagac ctggaactgg cggtgggtga ggctttcgcc aacgccgtgc
     1861 gccacggctc gccccgcggc gcggaggacc gtgtgacgtt gcgaaccctc tactccagcg
     1921 aggcgtttgt cgtcgagatc accgaccgcg ggcggggctt cgatcgctcg cagctgacac
     1981 tcccctctcc agaagagatc cgggagggcg gcatgggcat cctctatatg gagcaactca
     2041 tggatgccgc caccttcgag tcggggcagt ggggcacgac cgttcgtctg atcaaggcgc
     2101 tgcctcggcc cgcagagcga tcctgtctct aagcgccccc tcgctagccg ccgcggagga
     2161 tcgggatgcc gccttctgtc agtgtgatcc cggccgtctg cgcgagcgtg agcgccgccg
     2221 ttgggaacag cccaaggagc accacgagcg caaccgccat caaaaccgcc acgcgcaacg
     2281 agagcgaccg cgagagcgcc tcctgcttcc cctcggcacc cgaggccgcc ggggcttcca
     2341 tgtacatgta gcggacgacg cgcaggtagt agtagagcga gatcacgctc gccagcacca
     2401 ggaggacggc cagaccatcg aggcg
//
LOCUS       DAOSCV010000703         2419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28533_length_2419_cov_3.837140, whole genome shotgun sequence.
ACCESSION   DAOSCV010000703 DAOSCV010000000
VERSION     DAOSCV010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_2419_cov_3.837140"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PLY56_15190"
     CDS             complement(<1..1603)
                     /locus_tag="PLY56_15190"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ22871.1"
                     /translation="MPSLSGIVTPGALLLLALGPGAWGAGQAVGLKHPVLRHDVDPAR
                     AAQYEESVKRVMAMREDELLRFVPPYGYASYCECPNCYGGVEGNNIFEWSIDRPDELK
                     CRFCGTVVFPNPNYPENQFLIGKNRLDETIRLPYYLHPEKGVEHFFTLNRYLYQRKWL
                     LDQCLALSKAYHATGKGEYARRVAIVLDRFATVYPHYPVLQNYPRLLRFRESQEPPWP
                     WDSGRWGYFHNEIPTQVILCYDLIYDSEELDRLSRERGYDVRERIERDFLIPTFEAVV
                     AQKNHVGNVVGYDVRSAALLGRVINAPRMVHWAFGWMKENVNSGFMADGMWPESPSYH
                     YMTVGGLRYAFESVRGYSDPSGYRDPVTGARFDNLDPERELPFWAKVQHAPEAIGFPN
                     GWSNPVHDTHPYERRAKPRDRTVSTILPAFGHASLGRGTGPNQLMAQLHFSGGYGHAH
                     HDSLNLTLWAKEREMLPDLGYTWTQMRTWTTSTLSHNTVVVNRRDQRARDSDGDLLAF
                     FTDAAGIGVVEADGRRAYAHIDGLDL"
     gene            complement(1763..>2419)
                     /locus_tag="PLY56_15195"
     CDS             complement(1763..>2419)
                     /locus_tag="PLY56_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22872.1"
                     /translation="MKQKGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCQSN
                     LKQIGLAAMQYTQDYDETLVHYNLANIGGWDKMLEPYLKNTGVLRCPSGPQGVLAYGY
                     NYFYLGDGTANGTRSMASIQSPSETVAFADGAWNDTGGNYNPAHINPPHMTTYQYIAR
                     PAWRHGDGANVTFVDGHVKWYKAGSAFYPATAPLGDWTGNGITNPTDPNYKNQLWDRE
                     "
BASE COUNT          434 a          730 c          807 g          448 t
ORIGIN      
        1 agagatccag gccgtcgata tgggcatagg cgcgccggcc atccgcttcc acgacgccaa
       61 tccccgcagc atcggtgaag aaagcgagca agtcgccatc cgaatctctg gcgcgctggt
      121 cgcggcggtt gaccaccaca gtgttgtggc tgagggtgct ggtggtccag gtgcgcatct
      181 gcgtccaggt gtagccgagg tcggggagca tctcgcgctc tttggcccag agggtgaggt
      241 tgaggctatc gtggtgtgcg tgcccatagc cgccggagaa gtggagctgc gccatcaact
      301 ggttgggccc ggtgccccga ccgagggaag catgaccaaa cgccggcagg atcgtcgaga
      361 ccgtccggtc gcgcggcttc gcccggcgct cgtagggatg ggtgtcgtgg actgggttgg
      421 accagccgtt ggggaagcca atcgcctcgg gcgcgtgctg caccttcgcc cagaagggga
      481 gctctcgctc cgggtcgagg ttgtcgaagc gggcaccggt caccggatca cgatagccgg
      541 acgggtcgga gtagccgcgg acgctctcaa aagcatagcg aaggccgccg acggtcatgt
      601 agtgatagga cggcgactcg ggccacatgc cgtcggccat gaagccggag ttgacgttct
      661 ccttcatcca gccaaacgcc cagtggacca tgcgcggcgc gttgatcacg cgcccgagaa
      721 gcgcggccga gcgcacatcg taaccgacga cgttgcccac atggttcttc tgcgccacga
      781 cggcctcgaa ggtggggatg aggaagtcac gctcgatccg ctcgcggacg tcatagcccc
      841 gctctctcga gagacggtcc agctcctcac tatcgtagat caaatcgtaa cagaggatca
      901 cctgggtcgg gatctcatta tgaaagtagc cccagcgtcc ggaatcccag ggccaggggg
      961 gctcctgaga ctcacggaag cggagcagac gcgggtagtt ctgcaacacc ggatagtgcg
     1021 ggtagaccgt ggcgaagcgg tcgagaacga tggcgactcg ccgggcgtac tcgcccttgc
     1081 ccgtcgcgtg gtatgccttg ctcagggcca ggcactggtc gagcagccac ttccgctggt
     1141 agaggtagcg gttgagcgtg aagaagtgct caaccccctt ctccgggtgg aggtagtagg
     1201 gaaggcgaat cgtctcatca aggcggttct tgccgatgag aaactggttc tcggggtagt
     1261 tcgggttggg gaaaacaacg gtgccgcaga agcggcactt cagttcatcg ggccggtcga
     1321 tgctccactc gaagatgttg ttcccttcca cgccgccgta gcagttgggg cactcgcagt
     1381 aggatgcgta gccgtaaggg ggcacgaaac ggaggagctc gtcctcgcgc atcgccatca
     1441 cgcgcttcac ggactcttca tactgcgccg cgcgagccgg gtccacatcg tgccgcaaca
     1501 ccggatgctt gaggccaacc gcctggcccg cgccccacgc gccgggtccc agggcgagga
     1561 ggagaagcgc gcccggtgta acgattccgg agagtgaggg catcctggtg ctcctttctg
     1621 ccagtgacgg ctatcatctc cccggcccga taacgttctg tcgtcgtcgc gattctgaaa
     1681 taagccgggt tgcgcgtcgc ccgcgcggcg ccggccagcc cgagccgtgc tcgggccggc
     1741 cggcgcgata aggagcgcga gcttactcgc gatcccacag ctggttcttg tagttggggt
     1801 cggtagggtt ggtgattccg ttgcccgtcc agtccccaag cggcgccgtc gctgggtaga
     1861 acgcgctgcc cgccttgtac cacttgacat gcccgtcgac gaaggtgacg ttggcgccat
     1921 cgccgtgccg ccacgcaggg cgggcaatat actggtaagt ggtcatgtgc ggcgggttga
     1981 tatgcgccgg gttgtagttg ccgccggtgt cattccacgc gccatcggcg aaggctaccg
     2041 tctccgaagg gctctggata gaggccatcg accgggtacc attcgccgtg ccatcgccca
     2101 ggtaaaagta gttgtagccg tacgcgagca caccctgcgg gccggatggg catctcagca
     2161 cgcccgtgtt cttcagatag ggctcgagca tcttgtccca accaccgata ttcgccaggt
     2221 tgtaatggac cagggtctcg tcatagtcct gggtgtactg catcgccgcc aggccgatct
     2281 gcttcaggtt gctctggcag gtggacttgc gcgcgttctc ccttgctcgc gcgaacacgg
     2341 gaaagagaat cgccgccagg atggcaatga tcgctatgac gacgagcaat tcgatgagcg
     2401 taaatccctt ctgcttcat
//
LOCUS       DAOSCV010000704         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28637_length_2413_cov_3.688295, whole genome shotgun sequence.
ACCESSION   DAOSCV010000704 DAOSCV010000000
VERSION     DAOSCV010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28637_length_2413_cov_3.688295"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLY56_15200"
     CDS             complement(<1..897)
                     /locus_tag="PLY56_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ22873.1"
                     /translation="MASTITIEPVTRIEGHAKVTIHLDDAGKVTEARMHVNEFRGFEK
                     FCEGRMFFEMPQITQRICGICPISHHLASAKACDAIVGQELPRPARLIRELMHMGQFI
                     QSHALHLFHLASPDLLLGFDADPTIRNVTGVIQANPELALKGIGLRKFGQEVIRKIGG
                     KRIHPYDAATPNSVNKGLDPADRDELRSQIDGVVDGMRMAVAVIKGYAEANAEQMESF
                     AAFPSGYAGLVGDRGQLELYDGRIRMVDSKLRPWEPDYDPANYLNYIAEKTVPWSYLK
                     FPFYRKLGFPTGAYRVGPLARLN"
     gene            complement(1035..1571)
                     /locus_tag="PLY56_15205"
     CDS             complement(1035..1571)
                     /locus_tag="PLY56_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOJ22874.1"
                     /translation="MAKVKIATDWLAGCAGCHMSLLDLDEELVTILGEVELTSSPITD
                     LKHPPEVTVGIVEGAVANTANINTLKEMREKCQILLALGDCACFGGIPTMRNLVGTEE
                     ALKRAYIETPSTVDGEVPSDPELCQLLDRTRACNEVVKVDAYLPGCPPSAQAIGWAIK
                     ELLEGRLPEPVGENLRYD"
     gene            complement(1608..>2413)
                     /locus_tag="PLY56_15210"
     CDS             complement(1608..>2413)
                     /locus_tag="PLY56_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ22875.1"
                     /translation="TEALAALRSYAETALAPTNAVALGAQGIFDRTPDATMPDILAAD
                     LIILAGENATADHEVAGFLVNRAVEQNDTPLVLIGTATKRLVRRARVHLPVEGAALAE
                     ALRTLGQAGNPAAPLLTAAQRPLFLLCPPASEIVLKEVAAGAEAGWPRVLRLGEKANS
                     RGAAAAGLPANGVDWRAAKAILCLAGDDPGLSLEALGRMDGQLFLVVHSAYRTPLTER
                     ADVVLPAPAWFEKNGTMINAQGQEVHLRAAIEPPPGARDESEVLMALSS"
BASE COUNT          367 a          820 c          794 g          432 t
ORIGIN      
        1 gttgagccgg gccagcgggc cgacgcgata cgcgcccgtc ggaaaaccaa gcttccgata
       61 gaaggggaac ttgaggtagg accagggcac cgtcttctcg gcgatgtagt tcaggtagtt
      121 cgccgggtcg tagtccggct cccacggccg gagcttcgag tcgaccatgc ggatgcggcc
      181 gtcgtacagc tccagttgcc cccgatcccc caccaggccg gcgtacccag aggggaacgc
      241 ggcgaagctc tccatctgct cggcattcgc ctcggcatag cctttgatca cggcgacggc
      301 catgcgcatt ccgtcgacga cgccatcgat ttgcgagcgg agctcgtcgc gatccgccgg
      361 atccaggccc ttgttgacgc tgtttggcgt ggcggcgtca tagggatgga tccgcttgcc
      421 gccgatcttt cggatcacct cctgcccaaa cttccgcagg ccaatcccct tgagcgcgag
      481 ctctgggttc gcctggatca cgccggtcac gttgcggatc gtggggtcgg cgtcgaagcc
      541 gaggagcagg tccggcgagg cgaggtggaa caggtggagc gcgtgcgatt gaatgaactg
      601 ccccatgtgc atcagctcgc gaatcagccg ggctggccgg ggcagctcct ggcccacgat
      661 cgcgtcacac gccttggccg aggccaggtg gtggctgatc gggcagatcc cgcagatccg
      721 ctgtgtgatt tggggcatct cgaagaacat gcgcccctcg cagaacttct cgaagccccg
      781 gaactcgttg acgtgcatcc gggcctcggt gaccttgccc gcgtcatcga gatggatcgt
      841 caccttggcg tggccctcga tgcgcgtgac cggttcgatg gtgatggtgc ttgccatcct
      901 cttccctcca gagatggagc gcgagggggc gatgggcgat aggaggagag agcgtctttc
      961 cccggcagct ccctctaagg gcgctggcgc ctcccgggat gccgccgggg atcccctcgc
     1021 gtcccgcacc tcgatcagtc gtagcgcaga ttctctccca cgggctcggg gagccggccc
     1081 tccaggagct ccttgatcgc ccagccgatc gcttgcgcgg aaggagggca gcctggaagg
     1141 taggcatcca ccttcaccac ctcgttgcag gccctggtgc gatccaggag ctggcacagc
     1201 tccggatccg agggcacttc accatctacc gtgctcgggg tttcgatgta ggctctcttc
     1261 agcgcctcct ccgtgccaac gaggttgcgc atcgtcggga tcccgccgaa acaggcgcag
     1321 tcgccaagcg ccagcaggat ttgacacttc tcgcgcatct ccttgagcgt gttgatgttg
     1381 gcggtgttgg ccaccgcgcc ctcgacaatg cccaccgtta cctcgggagg gtgcttcaga
     1441 tccgtgatcg ggctggatgt gagctcgacc tcgcccagga tggtgaccaa ctcctcatca
     1501 agatcgagca gcgacatgtg acagccggcg caccctgcca gccagtccgt cgcgatcttc
     1561 actttcgcca ttctatctcc ccccatcttg tgccgcatct cgctacatca cgaggacaac
     1621 gccatcaaca cctcgctctc atcgcgcgcg ccgggcggcg gctctatcgc cgcgcggagg
     1681 tggacctcct ggccctgcgc gttgatcatc gtgccgtttt tctcgaacca cgccggggct
     1741 ggcaggacca cgtccgctct ctccgtgagc ggtgtacggt aggcggagtg gaccacgagg
     1801 aagagctggc cgtccatccg gcccagtgcc tccaggctga gcccgggatc atcgccggcc
     1861 aggcagagga tcgccttcgc cgcccgccag tcgactccgt tggccgggag accggctgcc
     1921 gccgcgcctc ggctgttcgc cttctcgccc agccgcagta cgcgtggcca gcccgcctcc
     1981 gcgccagccg cgacctcctt caggacgatc tcgctcgcgg gcgggcagag gaggaagagg
     2041 ggacgctggg cggccgtgag cagcggcgcc gccggatttc ccgcctggcc gagcgtgcgc
     2101 aacgcctccg cgagcgccgc gccctccacg ggtagatgca cccgggcgcg gcgaaccagg
     2161 cgctttgtcg ccgtgccgat caagacgagc ggcgtgtcgt tctgctccac ggcccggttc
     2221 accaggaaac cggccacctc gtggtccgcg gtggcgttct cgccggccag gatgatcagg
     2281 tcggccgcca gaatgtctgg catcgtcgca tccggcgtgc ggtcgaagat gccctgggcg
     2341 cctagcgcga ccgcgttcgt gggggcgagg gcggtttcgg cgtaggatcg gagcgcggcc
     2401 agcgcctcgg tat
//
LOCUS       DAOSCV010000705         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28653_length_2412_cov_3.843869, whole genome shotgun sequence.
ACCESSION   DAOSCV010000705 DAOSCV010000000
VERSION     DAOSCV010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28653_length_2412_cov_3.843869"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLY56_15215"
     CDS             <1..290
                     /locus_tag="PLY56_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ22876.1"
                     /translation="AGDILKRKKSLPIVYALTHGSAQERQTIIDVFSQPDLGPSDVER
                     VVQVLDRCGAPEYCARIMQEQGRQALAALDRLPDVPAVAQVRGLVEQVLAG"
     gene            324..1370
                     /gene="miaA"
                     /locus_tag="PLY56_15220"
     CDS             324..1370
                     /gene="miaA"
                     /locus_tag="PLY56_15220"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ22877.1"
                     /translation="MNAQGAGQAEHGSGKVPAHPEGPSSRSENTPAPAPHPERSSPPP
                     IAVITGPTAVGKTEIGVLVAEALGTEIVNADSMQVYREMHAGTAKPTPEERARVPHHL
                     VEVASVRDVFTVADFKELAIPILHRLIAEGKIPLVVGGTRLYIKALTSGFFEGPPRDP
                     EFRARMEALAAEHGRPYLHAMLAEIDPDKAAKLSPHDLKRIVRALEVHHATGQRISQL
                     QAQSQEAQPPFRAILIGLVRDREELYRRIDERVDQMVEAGLLEEVRALHEAGLDERLT
                     AIQAHGYKELIGYLRGEYDFAEAVRITKRNTRHYARRQLSWMRGEPGMHLLDASRPPE
                     VVATDATRIIRAES"
     gene            1493..>2412
                     /locus_tag="PLY56_15225"
     CDS             1493..>2412
                     /locus_tag="PLY56_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ22878.1"
                     /translation="MSFRIGRVWGFPIEIDVSWFIIFALVAWTLSAGYFPQLFPDAPA
                     SVNWVTGVISSLLFFASVLVHELAHSYVAIRNGLDVGGITLFLFGGVSRITREPRSPE
                     LELKMSAAGPATSVLLGVLFWGLGYGARSIGLPGHIVAAAGYLALINVILAIFNMVPG
                     FPLDGGRVLRALLWRFTGNLERASRYASYSGQGFGYLLMVGGVLFILSGNLIGGVWFI
                     FIGWFLVGAAQNAYQQVLLKRALSGIPIERIMQRDVPHLDPSLPIRDVVDDYLVRHDY
                     SAYPVMEGDQLVGILTIDEVRNVPRARWDAT"
BASE COUNT          388 a          805 c          799 g          420 t
ORIGIN      
        1 aggccggcga catcctgaag cggaagaaga gcctgccaat tgtctacgcc ctaacgcacg
       61 gatcggccca ggagcggcag acaatcatcg acgttttctc ccagccggat ctgggcccca
      121 gcgatgtgga acgcgtcgtg caggtgctgg atcggtgcgg cgcccccgag tactgcgccc
      181 ggatcatgca ggagcagggc cgccaggcac tcgccgccct ggaccgcctg ccggatgttc
      241 ccgcggtggc gcaggtgcgc ggcctcgtcg agcaggtgct cgccggctga agcatcacgg
      301 gagccgccgc gagggagacg cccatgaatg cccagggagc aggccaggcg gagcatggtt
      361 ccgggaaggt ccccgcgcat ccggaggggc catcatcacg gagtgagaac acccccgcgc
      421 ctgctccgca tccggagcga tcctctccgc cccctatcgc ggtgatcact ggcccgacgg
      481 ccgtcgggaa gacggagatc ggcgtgctcg tcgccgaggc gttgggcacg gagatcgtga
      541 acgcagactc gatgcaggtg taccgcgaga tgcacgcggg caccgcgaag ccaaccccgg
      601 aagagcgcgc gagagtccca catcacctgg tggaggtcgc ctcggtgagg gacgtcttca
      661 ccgttgccga tttcaaagag ctggcgatac cgatcctgca ccggctgatc gcggagggga
      721 agatcccgct ggtggtcggt ggcacccgcc tctacatcaa ggcgctcact tcgggtttct
      781 tcgagggccc gccacgcgac cctgagtttc gcgcgcggat ggaggcgctc gccgcggagc
      841 atgggcggcc ctacctgcac gcgatgctcg ccgagatcga ccccgacaag gcggcgaagc
      901 tctcgccgca cgacctgaaa cggatcgtgc gcgccctgga ggtacaccac gccaccgggc
      961 agcgcatcag ccagctccag gcgcagagcc aggaggcaca gccgcctttc cgggccatcc
     1021 tgatcggttt ggtgcgcgac cgcgaggagc tctaccggcg catcgacgag cgcgtggatc
     1081 agatggtgga agccggcctc ctggaggagg tgcgcgcgct tcatgaggcc ggcctcgacg
     1141 agcgcctgac cgcgatccag gctcacggat acaaggagct gatcggttac ctccgcgggg
     1201 agtacgactt cgcggaggcg gtgcgcatca ccaagcggaa cacccgccac tatgcccgcc
     1261 gccagctctc ctggatgcgc ggggaaccgg gaatgcacct gctcgacgcc tcgcgtcccc
     1321 cggaggtggt cgccaccgac gccacgcgca tcatccgcgc ggaatcctga tccgcatctc
     1381 cccctccctg cccgtccgcc gccgctctca aggtttgcac gccgtactga cagggaacgc
     1441 gtcagaccgg gaatgaacgt atggcgcgct tcccaggaaa ggcgggcatg ggatgagctt
     1501 tcgcatcggc agggtttggg ggttcccgat cgagatcgat gtctcgtggt ttatcatctt
     1561 cgccctggtg gcgtggacgc tctctgccgg ctacttcccc cagctcttcc cagacgcgcc
     1621 tgcctcggtt aactgggtga ccggggtgat ctcgtcgctg cttttcttcg cgagcgtgct
     1681 cgtgcatgag ctggcgcact cctatgtggc gatccgcaac ggattggatg tcggcgggat
     1741 cacgctcttt ctcttcggag gcgtcagccg aatcacccgg gagccacgca gtccggagct
     1801 ggagttgaag atgtctgcgg ccgggccggc cacgagcgtc ctccttggcg tcctcttctg
     1861 gggcctgggc tatggcgcgc ggagcatcgg tctaccgggg catatcgtgg ccgcagctgg
     1921 ttacctggcc ctgatcaacg tgatcctggc gatcttcaac atggtgcctg gcttcccgct
     1981 ggatggtggg agggtgttgc gcgccctcct gtggcgattt acgggcaacc tggagcgcgc
     2041 cagccgctac gcatcctatt ccggccaggg ctttggctac ctcctgatgg tcggcggcgt
     2101 ccttttcatc ctgtccggca acctgatcgg aggcgtctgg ttcatcttca tcgggtggtt
     2161 cctggttggc gccgcccaga acgcctacca acaggtgttg ctgaagcgcg cgctttctgg
     2221 catccccatc gagcggatca tgcagcgcga cgtgccccac ctcgatccga gcctgccgat
     2281 ccgcgacgtg gtcgacgact acctggtccg ccacgattac agcgcttatc ccgtgatgga
     2341 gggtgaccaa ctcgtgggca tcctcaccat cgatgaggtg cgcaacgtgc cgcgcgcgcg
     2401 ctgggacgcc ac
//
LOCUS       DAOSCV010000706         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28701_length_2410_cov_3.268365, whole genome shotgun sequence.
ACCESSION   DAOSCV010000706 DAOSCV010000000
VERSION     DAOSCV010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28701_length_2410_cov_3.268365"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1671
                     /locus_tag="PLY56_15230"
     CDS             <1..1671
                     /locus_tag="PLY56_15230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22879.1"
                     /translation="QIAARTGKSLSYFYAQGDDFEEQVEKAQAELVEERKRIQQEIER
                     LQAERADLERRRLREAFEHLRELARAQELPADLKGLISTCERIISLGRELDDLRAVAD
                     AHLRIGNARIRLGEHDSARASLERAIDLFHQGGNREEELSARLSLANVLLQLGRPEAA
                     LEQSRMVAEAEDWWSRWKGTLCIGCVYEHLGEYRQALEQFDAALQIIDTSPDEQAATF
                     GRLYVHANIANVYIDCGDYEAAAAIARECQDDAEKLSHREQHIEAQINLGVCAFALGR
                     LGEAHQRLHRALEIARFVGDAGRTAVTRAWLCGLYASIGQYDTAKELGKDALQLALQT
                     GSQRSVLYAHQHLGDAYLRSGRPREALYHYRQATSIARDQHVPYYEALLTERSGVAEL
                     RLGQPERAATLCGTALELSDRIGAREIEAAAHVGLSLAGLALGRMEEASRSAQRAIEI
                     AVEIEVPELQARGHGALARIALERGEVGVADEHSAAAVARIDRLRADVRSHGLEDGLL
                     EEEEREQVYLERARILALLGREEEARRFLEGAEWPPLERRFTAERASP"
     gene            1668..2318
                     /locus_tag="PLY56_15235"
     CDS             1668..2318
                     /locus_tag="PLY56_15235"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ22880.1"
                     /translation="MNARLRLGLGFLRRALAGVAACLLLMALSGCLTDGSIPVGGGGS
                     TGSVRGVVVFAEDPDAPLAGARVSLRTPGGLPYRTTTDDEGRFEFENVPKGTLEATFE
                     GAGRLPVRVFVETDFGSFSTIAVALAPAHLGIDDVAGVAVTPQDVKVRVGESVPFQVS
                     ISGGVPVRKVPTWIVEGGIGSIDAHGRFRALRAGTGTVRAVAGNFQDSARVTVLAP"
BASE COUNT          377 a          790 c          860 g          383 t
ORIGIN      
        1 cagattgccg cgcgcaccgg aaagagcctc agctacttct acgcccaggg cgacgatttc
       61 gaagagcagg tcgagaaggc gcaggcggag ctggtcgagg agcggaagcg catccagcag
      121 gagatcgagc ggcttcaggc cgagcgtgcg gatctggagc gccggcgact gcgcgaggcg
      181 ttcgagcacc tgcgcgaatt ggcgcgcgct caggagttgc cggcagacct gaaggggctc
      241 atctccacct gcgagcgcat catctccctg gggcgtgaac tggatgacct ccgggcggtg
      301 gcagacgcgc acttgcggat tggcaacgcg cgcatccgtc tcggcgagca tgattcggcg
      361 cgggcatccc tggagcgcgc aatcgatctc ttccatcagg gtgggaaccg cgaggaggag
      421 ttgagcgccc gtctgagtct tgcgaacgtg ttgctgcagc tcgggcgtcc ggaggctgcc
      481 ctggagcagt cgcgcatggt cgcggaggcc gaggattggt ggagccggtg gaaaggcacg
      541 ctctgcatcg ggtgtgttta cgagcatctc ggagagtatc ggcaggcgct ggagcagttt
      601 gacgccgcgc tacagatcat cgacaccagc cccgatgaac aggccgccac cttcggccgc
      661 ctctatgtgc atgccaacat cgccaacgtc tacatcgact gtggcgatta cgaggccgcc
      721 gcggcgatcg cccgcgagtg ccaggatgac gcagagaagc tgagccatcg cgagcagcat
      781 atcgaagcgc agatcaacct cggggtgtgc gcgtttgccc ttggccggct cggcgaagcc
      841 caccagcgcc tgcaccgcgc cctggagatc gcccgctttg tgggcgatgc cgggcgcaca
      901 gcggtgacgc gcgcctggct gtgcgggctt tacgcaagca tcggccagta cgacaccgcc
      961 aaggagctgg gcaaagacgc cctgcaactc gcgctgcaaa ccggctcgca gcgctcggta
     1021 ctctatgcgc accagcacct gggcgacgcc tacctgcgca gtgggcgccc gcgcgaagcc
     1081 ctctaccact atcgccaggc cacgagcatc gcccgcgacc agcatgttcc ctattatgag
     1141 gcacttctga ccgagcgcag cggcgtcgcg gaactgcgcc tgggccagcc ggagcgcgcc
     1201 gccacgctgt gcggcaccgc gctggagctt tccgaccgca tcggcgcgcg cgagattgag
     1261 gccgccgcgc acgtgggatt gtccctcgct ggactggccc tcggccgcat ggaggaggcg
     1321 agtcgctcgg cgcagcgcgc gattgagatc gcggtcgaga tcgaggtgcc ggagctgcag
     1381 gcccgcgggc acggggctct ggcgcgcatc gcgctggaga ggggcgaagt gggcgttgcc
     1441 gacgagcact cagccgcagc ggttgcgcgc atcgaccgcc tccgtgccga tgtgcgtagc
     1501 cacgggctcg aggatggcct gctggaggag gaagagcgag agcaggttta cctggaacgc
     1561 gcccgtatcc tcgcgctgtt gggtcgtgag gaggaagcgc gccggttcct ggagggggcc
     1621 gagtggccgc ccctggaacg tcgattcacc gcggagagag cttcgccgtg aacgcgcgcc
     1681 tgcgtctcgg gttgggcttc ttgcgccgtg ccctcgccgg cgtcgccgcg tgcttgctcc
     1741 tgatggccct ctcgggatgc ctgaccgacg gcagcatccc ggtgggcggt ggggggagca
     1801 ccggatccgt gcgcggcgtg gtggtcttcg cggaggatcc ggacgcgccg ctggccggcg
     1861 cgcgggtctc cctccggaca cccggcggac tcccctaccg caccaccacg gatgacgagg
     1921 gccgcttcga gtttgaaaac gttcccaagg gaacgctcga agccaccttc gagggggcag
     1981 gtcgcctccc cgtgcgcgtc tttgtagaga cggacttcgg ctcgttctcg accattgccg
     2041 tggcactcgc gcccgcccac ctcgggatcg atgatgtcgc cggcgtggca gtcacccccc
     2101 aagacgtgaa ggtcagggtc ggtgagagcg tgcccttcca ggtgagcatc tccgggggcg
     2161 tgcctgtgcg caaggtgcct acgtggatcg ttgagggggg catcggcagc atcgacgccc
     2221 acggccggtt ccgggccctc cgcgccggta ccggcactgt acgcgccgtg gccggcaact
     2281 tccaggattc cgctcgggtc acggtcctcg cgccttgatt ccacgaatcg ggggccgcgc
     2341 gcgatcccgg tcccctcgcg ggaggagagg gccggcggtg taaacgcctc cttccagcag
     2401 cctttgcatg
//
LOCUS       DAOSCV010000707         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28727_length_2408_cov_3.526137, whole genome shotgun sequence.
ACCESSION   DAOSCV010000707 DAOSCV010000000
VERSION     DAOSCV010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28727_length_2408_cov_3.526137"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLY56_15240"
     CDS             complement(<1..637)
                     /locus_tag="PLY56_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22881.1"
                     /translation="MRQRQSTAGFTLIELLVVIAIIAILAAILFPVFARARENARKAN
                     CQSNLKQLGTAFTSYAQDYDETLPSFNAYFSPPMFFQTLLEPYLKNTEVWTCPSARNK
                     YWATYGVPPEMVTNRTVTYGVNDIFLLPGNGISANDFFGRARVAKTMAMIERPAEVFV
                     LGDSGYYTIWMRTLTGGTLQMDSAGLNRIRFPHGEGANFAFADGHVKWYNEQ"
     gene            complement(978..1667)
                     /locus_tag="PLY56_15245"
     CDS             complement(978..1667)
                     /locus_tag="PLY56_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ22882.1"
                     /translation="MNQTALVTVRDVTKLFSTGRSEVQALRGVTLDVRRGDFLALVGP
                     SGSGKTTLLNLIGALDSATSGTITLLGRDLGAMNRRERAELRLHTIGFVFQAYNLIPV
                     LTARENVEFVLELQGVGAERSERALRVLEELGLGELADRRANELSGGQQQRVAVARAV
                     ASRPALVLADEPTANLDTANAEVLMGLMRRLHDEHGMTFVFSTHDPRVVAHATRVVTL
                     QDGRVAADEER"
     gene            complement(1664..>2408)
                     /locus_tag="PLY56_15250"
     CDS             complement(1664..>2408)
                     /locus_tag="PLY56_15250"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ22883.1"
                     /translation="DELLVTVAARDGNLQSAILRIVGIVRTGSQTVDSTVCQIRLPEL
                     EALTGRPGAAEVAVLLERSEEMPAVQAALQKALPAGNVVHTWIEVLPELKASFDLDQG
                     FTRLLIGTIILVVLLGITSAQLASVLERRREFAVLSALGMKELSIARLLLLEAGALGL
                     LGAVTGLLLGVPVVYYLATVGIDYGAFVDADLAISNVLIDKVFHADMGFWLVPQALAI
                     ALGATLVATVYPVWFAAKTDPASALRVAQ"
BASE COUNT          453 a          800 c          755 g          400 t
ORIGIN      
        1 cctgctcgtt ataccacttc acgtggccgt ccgcgaaggc gaagttggcc ccctccccat
       61 gcgggaagcg aatccgattg agcccggcgg aatccatttg cagcgtgcca ccggtcagcg
      121 tccgcatcca gatcgtgtag tacccagagt cgccaaggac gaagacttcc gccgggcgtt
      181 cgatcatcgc cattgtcttt gccacccggg cgcgcccgaa gaagtcgttc gcgctaatac
      241 cgttgcccgg gagcaagaag atgtcgttga cgccataggt gaccgtcctg ttggtcacca
      301 tctccggcgg gacgccatag gtcgcccagt acttgttgcg agcgctcggg cacgtccata
      361 cctcagtgtt cttcagatac ggctccagca gggtttgaaa gaacatcggg ggagagaagt
      421 aggcgttgaa ggacggcagg gtctcgtcat agtcctgagc ataggaggtg aacgcggtac
      481 cgagctgttt caggttgctc tggcagttcg ccttgcgcgc attctcgcga gccctggcaa
      541 acaccgggaa gaggatcgca gccaggatcg cgatgatggc gattacgacg agcagctcaa
      601 tgagcgtgaa accggcggtt gactgacgct gacgcatagg aaccatcctt ctcggccgat
      661 ggccgcaggg ctgactaggg gtctctgcgt acactcagac ggcgctacac gcaacaaaag
      721 cacagaacgg atgggatgtg tccagattgc gcgcgtcgat catcggatcg atccgtgccc
      781 ggcaaagccg ggtcacgccc atcggtcccg ttccgtccgt gccttgcgag cgccggcagc
      841 aaaccccgat ggtcagccac acaatcgatt tgcgcgcatt gtagcacccc cggcaagcgc
      901 ctgtcaaccc atcaaccgtg cgtttttcgg acgggcagca attcggttcg cggatgctat
      961 tcgcgcggcc gctcgggcta gcgctcttcg tcggctgcga cacgtccgtc ttgtagcgtg
     1021 acgacgcgcg tggcgtgggc gacaacgcgc ggatcatgcg tggagaagac gaaggtcatg
     1081 ccgtgctcgt catgcaggcg gcgcatcagt cccattagta cctcggcgtt ggccgtatcc
     1141 agattcgccg tcggctcgtc ggccaggacc agcgccgggc gcgaggcgac agcgcgcgcg
     1201 acggccaccc gctgctgctg gcccccggag agctcattcg cccggcgatc cgcaagctcc
     1261 cccaggccca gctcttccag gacgcggaga gcgcgctcgc tcctctcagc acccacgccc
     1321 tgaagctcga ggacgaactc gacattctcg cgcgcggtga ggacggggat caagttgtac
     1381 gcctggaaga caaacccgat cgtgtgcagg cgtagctccg cgcgctctcg ccggttcatc
     1441 gcccccagat cccgcccgag aagcgtgatc gtgccgctcg tggcgctgtc gagcgcgccg
     1501 atcaggttga gcagcgtcgt cttgccgctc ccgctggggc cgaccagagc caggaagtcg
     1561 ccgcggcgca catccagtgt caccccgcgc agcgcctgga cttcactccg gcccgtggaa
     1621 aagagcttgg tgacatcgcg caccgttaca agagctgtct ggttcattgg gccacccgaa
     1681 gcgccgaggc gggatccgtc ttcgccgcaa accacaccgg gtagaccgtg gcaaccagcg
     1741 tggctcccaa cgcgatggca agcgcctgcg gcaccagcca gaaccccata tcggcgtgaa
     1801 acaccttgtc aatcagcacg ttggagatgg cgaggtccgc gtcgacaaac gcgccatagt
     1861 caatcccgac tgtcgccagg tagtagacta ccggaacgcc caggagaagc ccggtcacgg
     1921 cgcccagcag gccaagcgcc ccggcttcca ggaggagcag gcgtgcgatc gagagctcct
     1981 tcatccccag cgcggagagg acggcgaact cgcgccggcg ctccagcaca gacgcgagct
     2041 gcgcgctcgt gatcccaagg agcacgacga ggatgatcgt tccgatgagc agccgcgtga
     2101 agccctggtc caggtcaaag ctggccttca gctccgggag gacctcaatc caggtatgca
     2161 ccacgtttcc ggcaggcagc gctttctgca acgctgcctg cacagccggc atctcctcgg
     2221 agcgctccag gagcacggcg acctccgccg caccggggcg cccggtgagc gcctccagct
     2281 ccggcaggcg gatctggcag accgtggaat ccacggtctg gctgccagtg cggacaatgc
     2341 cgacgatccg caggatcgcg ctctgcaggt tgccgtcgcg cgccgctacg gttaccagca
     2401 actcatca
//
LOCUS       DAOSCV010000708         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28795_length_2404_cov_3.043848, whole genome shotgun sequence.
ACCESSION   DAOSCV010000708 DAOSCV010000000
VERSION     DAOSCV010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28795_length_2404_cov_3.043848"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1263)
                     /locus_tag="PLY56_15255"
     CDS             complement(31..1263)
                     /locus_tag="PLY56_15255"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOJ22884.1"
                     /translation="MTRQLSCLVGLVALAALPCNGGPRAAESAAAKVGVRANDPGTWK
                     FSLQAMAGGLLQKLHQTRPVANRPGVQVASARKTPPRTSQRATGSLRVESKPTGATVL
                     VDGKERGKTPAALKLYEIGKAARDVRVEARLDGYLPEAATARLAQGAQKTLRFTLRKW
                     PPDVPPPGRHKFQTWTNPKDGSEFVFVPASEFIMGSDEGDNKFANSAHKVYLPGYWIG
                     KYEVTLGQFRKFMAETGHEPEGNIDENGIDDCLPVVGVTWGDAMAYARWAGCRLPTEA
                     EWEKAARGTDGREFVWGNAWPPPRGAGNFWEKGIKGYNDGFIGPAPVGSFPGGASPYG
                     CLDMAGNVFEWTSSLWKPYPYRADDGREDPRMDDERVFRGGCWGGDRPDDHHAYRRFC
                     DTVHRERFLGFRLARSVE"
     gene            complement(1544..2284)
                     /locus_tag="PLY56_15260"
     CDS             complement(1544..2284)
                     /locus_tag="PLY56_15260"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOJ22885.1"
                     /translation="MGLGEREQTLVLIKPDALKVSLTGYILSQFSEFHTGLRFAGSKI
                     VHVSRMIAEEHYAEHRGKGFYPSLIDYIMGRLHYPMEPEKRRVIALVYSGPDAIRKVR
                     QITGPTNPHVARDNAPGTIRALGTVVPIKDAEGNVVGDRMDNLVHASANEADAEREVK
                     LWFRPDEIMPYMRAYPTEVASKYYFYKDGQLLTQEEAGSVCLVAPGDLVWASDLDALE
                     NLLQGKPGPVTLESVAAKYLLNREGEPA"
BASE COUNT          382 a          872 c          701 g          449 t
ORIGIN      
        1 acatccgtgc cgccaccttc acccgtggaa tcactccacg gacctggcaa gccggaaccc
       61 caggaagcgc tccctgtgga cggtgtcaca gaaccgcctg tacgcgtggt ggtcgtcagg
      121 gcggtcgcca ccccaacacc cgccgcgaaa cactcgctcg tcatccatgc gtgggtcctc
      181 gcgcccgtcg tccgcccggt atggatacgg cttccagagg ctactcgtcc actcaaagac
      241 gttccccgcc atatccaggc acccgtaggg cgacgcccca cccgggaagc tccccaccgg
      301 cgccggcccg ataaacccgt cgttatagcc cttgatgcct ttctcccaga agttccccgc
      361 gcctctcggc ggcggccaag cgtttcccca gacaaactcg cgcccatcgg tgccgcgcgc
      421 cgctttctcc cattccgcct ccgtgggcag ccggcacccc gcccaccgtg cgtaggccat
      481 cgcgtcaccc cacgtcacgc ctaccaccgg caggcagtca tcgatgccgt tttcatcgat
      541 gttcccctcc ggctcgtgcc ctgtctccgc catgaacttg cggaactggc cgagggtcac
      601 ctcatacttc ccgatccagt atccgggcag gtagaccttg tgcgctgaat tcgcaaactt
      661 gttgtcaccc tcatcgctgc ccatgatgaa ctcactggca ggcacgaaga cgaactcgct
      721 cccgtccttc gggttggtcc aggtctggaa cttgtggcgg cccggtggcg gcacatcggg
      781 cggccacttc cgcagggtga aacgtagtgt cttctgcgca ccttgcgcca gccgggccgt
      841 ggccgcctcg ggcaagtagc catccaggcg cgcctctacg cgcacatccc gcgcagcctt
      901 gccaatctca tacagcttta gcgccgcggg cgtcttcccc cgttctttcc cgtccactag
      961 caccgtcgcc ccggtgggct tgctctccac gcgcagcgat ccggtggcgc gttgggacgt
     1021 ccgtgggggc gtcttacggg cgctggccac ctgcactccg ggcctgttcg ccaccggccg
     1081 cgtctgatgc agcttctgga gcagcccccc ggccatcgcc tggaggctga acttccaggt
     1141 gccgggatca ttcgcgcgca ctcctacttt ggccgcagcg ctttccgcgg cccgcgggcc
     1201 cccgttgcag gggagcgccg caagagcaac caatccgaca aggcagctca actggcgtgt
     1261 catggtagcc ctccgcgcat gagaaaaccc ccttgccctc gacaagcggg cgcaactgtc
     1321 ggccaattat agcaccgggc tcttacccga gcaactgttt gtttgcgaaa caaaatccgg
     1381 gctgcgtgta tcgcgcggag cccgcgtgcc gtgcgggtgc gagcctctcc gcttcgtcct
     1441 gattccgggg cgcggtgcac tcgtggcagg caggtgcccg gtatacgcca gagggccggc
     1501 caggtggccg gccctcgtca ccagatcgcg ctcgcccgcg gcgtcaggcg ggttcgccct
     1561 cgcggttcag cagatacttg gccgccaccg attcgagcgt gaccgggccc ggcttcccct
     1621 gcaacaggtt ctccagcgcg tccaggtcag atgcccacac gaggtcgccc ggagcaacga
     1681 ggcagacgct ccctgcctcc tcctgggtga gcagctggcc atccttgtag aagtagtact
     1741 tgctcgccac ctcggtcggg taggcgcgca tgtagggcat gatctcgtcc ggtcggaacc
     1801 agagtttgac ctcccgctcc gcgtcggcct cgttagcgga ggcatggacc aggttgtcca
     1861 tgcggtcgcc aaccacattc ccttccgcgt ccttgatagg aaccacagtg ccgagcgcgc
     1921 ggatcgtgcc tggtgcattg tctcgggcga catgcgggtt ggtggggccc gtaatctggc
     1981 gcaccttccg aatggcatcc gggccgctat ataccagcgc gatgacccgc cgcttctccg
     2041 gctccatcgg gtagtgaagc cggcccatga tgtagtcgat cagcgacgga tagaagcctt
     2101 tgccccggtg ctctgcgtag tgctcctccg cgatcatgcg gctgacgtgg acgatcttcg
     2161 acccggcgaa gcgcaacccg gtatgaaact ccgagaactg cgagagaata taccctgtca
     2221 gcgatacctt tagcgcatcc ggcttaatca ataccagcgt ctgctcacgc tcaccaagcc
     2281 ccatctgatt cctccaagcg tgtctgatca agggcgggga tactcccctc gcccatgcac
     2341 cctcatcgct caatcccgcg cccggtgggc ctatccccct cgcgaggggc gaggggggtt
     2401 ccgc
//
LOCUS       DAOSCV010000709         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28806_length_2404_cov_1.924649, whole genome shotgun sequence.
ACCESSION   DAOSCV010000709 DAOSCV010000000
VERSION     DAOSCV010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28806_length_2404_cov_1.924649"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /gene="pstC"
                     /locus_tag="PLY56_15265"
     CDS             complement(<1..741)
                     /gene="pstC"
                     /locus_tag="PLY56_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOJ22886.1"
                     /translation="MEGLRERLIGRTLLVVACSVIIMLAIITYFIFQAGLPLIVRLGL
                     DDFLFNPHWMPAEKGGSFGIGAMVLGSLWVTLGALIVGVPLGLAVAVFSAELAPEWLS
                     TVIRPAIQLLAGIPSVIYGFVGLTVLVPWVRAHLGGPGLSVLTAAVVLGIMILPNIIS
                     ISEDALRAVPPAYREGALALGSTRWQMIWRVLIPAARSGIAASVILGMGRALGETMAV
                     IMMIGNSLQVPRSLLDPATTMTSNIGLEM"
     gene            complement(729..1613)
                     /locus_tag="PLY56_15270"
     CDS             complement(729..1613)
                     /locus_tag="PLY56_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ22887.1"
                     /translation="MRPDQTSTTRRSPRPAVALFLASIALALSMFSGCSRTRGQETTQ
                     ITAAGSTSVTPFAEQLAEEFMRAHPGVHVNIQGIGSTAGIQAVQTGAADIGMSSRDLL
                     QEEERTLEPIVMALDALAIIVHPSNTVRNLTLEQIRRIFAGEIRDWSEVGGPAAPITL
                     VNREAGSGTRGAFEELVMGKNGPPLSYRSIRQGSNGAVRAVVEGDPRAIGYISLGIVN
                     SRVRTIAVDGVLPTAESVEAGRYRLVRPFLMVVARGGELPKLSRDYIAYVLGDEGQRS
                     LASIGLIPAPRKETPWKD"
     gene            1880..2329
                     /gene="dtd"
                     /locus_tag="PLY56_15275"
     CDS             1880..2329
                     /gene="dtd"
                     /locus_tag="PLY56_15275"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ22888.1"
                     /translation="MRAVIQRVRRGRVVVDSETVGEVGHGLVALIGVAEDDTLQDARY
                     IAEKIAGLRIFEDRDGKMNLSVKEIGGGVLAVSQFTLLGDARKGRRPSFSGAAAPERA
                     EALYLQVADLLEEQGIPVALGRFRAHMQVELLNDGPVTILLDSRRLF"
BASE COUNT          469 a          749 c          789 g          397 t
ORIGIN      
        1 catctccagg ccgatgttac tggtcattgt ggttgccgga tccagcaacg agcgcggcac
       61 ttgcagcgaa ttgccgatca tcatgatgac ggccatcgtc tcgccaaggg cacgccccat
      121 cccaaggatc acgctcgcgg cgatgccgga tcgcgccgcc ggaatgagca ctcgccagat
      181 catctgccaa cgagtggatc cgagcgccag cgcaccttcg cggtaggcgg gcggaacggc
      241 acgcagcgca tcctcggaga tgctgatgat gttgggaagg atcatgatcc caaggactac
      301 cgccgccgtc agcacgctca gaccggggcc gcccaggtgt gcgcgcaccc acggcaccaa
      361 tacggtgagc cccacgaagc cgtagatcac ggaggggatc cctgccagca gctgaatcgc
      421 cggccgaatc accgtcgaca gccactccgg cgccagctct gccgagaaga ccgccacggc
      481 aagtccgagc ggcacgccga cgatcaacgc gcccagcgtg acccacagcg agccgaggac
      541 catggcaccg atgccgaagc ttccgccctt ctccgcgggc atccagtgcg gattgaagag
      601 gaagtcatcc agccccaggc ggacgatcaa gggcaggccg gcctggaaga tgaaataggt
      661 gatgatggcc agcatgatga tcaccgagca ggccaccacg agaagcgtgc gcccgatgag
      721 gcgctctctc aatccttcca aggtgtctcc ttgcgaggcg ccgggatgag gccgatactc
      781 gccaacgagc gttgcccttc atccccgagc acgtaggcga tgtagtcgcg gcttaacttc
      841 gggagctcgc caccgcgggc cacgaccatg aggaaggggc gcacaagacg gtagcgccca
      901 gcctcgacgc tctcggcagt gggcagaacc ccatccaccg cgattgtacg tacccgcgaa
      961 ttgacaatcc ccaacgagat atagccgata gcgcgcgggt cgccctccac taccgcgcgc
     1021 accgcgccgt tcgatccctg ccggatggag cggtagctga ggggcgggcc attcttgccc
     1081 atgaccaact cctcgaaggc gccacgcgtg ccggatcccg cctcgcggtt gaccagggtg
     1141 atcggcgcgg ccggtccgcc tacctcgctc caatcgcgga tctcgcccgc gaagatgcgg
     1201 cggatctgct ccagggtcag gttgcgcacg gtgttcgaag ggtgaacgat gatcgccagc
     1261 gcgtcgagcg ccatgacgat cggctccagc gtgcgctcct cctcctgcag taagtcgcgc
     1321 gatgacattc cgatatcggc ggcgcccgtc tgcactgcct gaatgccagc cgtggagcca
     1381 attccctgaa tattcacatg aacacccgga tgcgcgcgca tgaactcctc ggcaagctgc
     1441 tcggcaaaag gagtcacgct agtcgagccc gcagcggtga tctgcgtcgt ctcctggccg
     1501 cgtgttcggg agcagcccga gaacatactc agagccaggg cgatgctggc aaggaaaagg
     1561 gcgacggccg ggcgtggcga tcgccgcgtt gtggaggtct ggtcaggtct cataaatctc
     1621 gtatgcttgc cgcagtcgtg gtgcgcggga acccgtcata tggcccagac gcggcatagc
     1681 gccggcgcca acgcccttcc gaacagaacg cgacaccctc tggggttgac tggcatctcc
     1741 agttcgatgc aggctggcga tctcgcccct gcgacaggta ttcttgcaca aatcctgcaa
     1801 cctgtcaacc cggcggcagg tttacaccgc taccgggatc gtgctacaat atcggcgaga
     1861 agagaggtga cgtgcgctca tgcgcgctgt gattcaaaga gttcgccggg gaagagtggt
     1921 cgtcgattcc gagacagtag gtgaggtcgg gcacggtctg gtggccttga tcggggtcgc
     1981 ggaggacgat acgctccaag atgcgcgcta cattgcggaa aagatcgccg ggctgcgcat
     2041 cttcgaggac cgcgacggca agatgaacct ctccgtcaag gagatcggtg gaggcgtgct
     2101 ggcggtctcg cagttcacgc ttttgggcga cgcgcgcaag ggacggcgcc ccagcttctc
     2161 tggggcggct gcgccggagc gcgctgaggc gctctatctt caggtggcgg acctcctgga
     2221 ggagcagggg atcccggtgg cgttgggccg gtttcgtgcc catatgcagg tggaactgct
     2281 gaatgatggt ccagtgacga tcttgctgga cagcagacgg ctattctgag agaaacgtgg
     2341 tgggaaggta gggagaggtc agttcgatgg gatcggatcg agacgcggtg gaggacctga
     2401 ggcg
//
LOCUS       DAOSCV010000710         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28852_length_2401_cov_3.591645, whole genome shotgun sequence.
ACCESSION   DAOSCV010000710 DAOSCV010000000
VERSION     DAOSCV010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28852_length_2401_cov_3.591645"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLY56_15280"
     CDS             <1..248
                     /locus_tag="PLY56_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22889.1"
                     /translation="MADAREVWADKVLWINYPSSVHLASIETIEETTRQILRDAAPGD
                     RLLVGITEDVPENRWRENFLAISRVLDTDGRTPVAAS"
     gene            complement(351..557)
                     /locus_tag="PLY56_15285"
     CDS             complement(351..557)
                     /locus_tag="PLY56_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22890.1"
                     /translation="MMVELDAQEFQGAIGDAVKVLRQVANDESQDGETRVEAAVALLE
                     FATHLHSSAGPEGWYEEYEEEEEK"
     gene            complement(592..1485)
                     /locus_tag="PLY56_15290"
     CDS             complement(592..1485)
                     /locus_tag="PLY56_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOJ22891.1"
                     /translation="MRAASWVLILAAATWIASAAAETPSQARAPASQNPPRLVVNGRM
                     VQTDIAPRIIQGTTLVPIRFIAQALGANLTWQPAGREAVIDYEGQRVVVQAGAETLVA
                     GNRSIRTRVPVQVIQGRTMVPLRVVAETFGADVRWDPQARTIYIRTVDQPAAPPSPEE
                     PAQPTPAAQIQQSVEVGLSTPQAEYPAGAPIELTLRIRNRGSETVRLLMRTGQLFDFI
                     VQRNGEVVWRWSEGRAFTQAVTQLVLLPDETRAFTVRWDGTDNTGARVPPGSYTVTGR
                     VTHALEVPLQDTREIRITAAP"
     gene            complement(1655..1978)
                     /locus_tag="PLY56_15295"
     CDS             complement(1655..1978)
                     /locus_tag="PLY56_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22892.1"
                     /translation="MEWTAFKAIRAPYSREVWELTLEELAACPLCSGTTFLIPFPAGG
                     ASAAGLEGASPHELPPLDAAIFLCFNEDCLSIYVIIFSEERREEFEALIPYLREAGRR
                     ALGDA"
     gene            complement(2092..>2401)
                     /locus_tag="PLY56_15300"
     CDS             complement(2092..>2401)
                     /locus_tag="PLY56_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22893.1"
                     /translation="KGSILKWGGLRLYRTLLPFFLGLILGDIMMGCLWSLIGIALGVE
                     TYSLGRLARIIHQNNEMKRSLSGVAQVCGRCPRKHSAAGRLWRQRSATRQETSLEVFW
                     "
BASE COUNT          404 a          829 c          748 g          420 t
ORIGIN      
        1 gcatggccga tgcgcgcgag gtgtgggcgg ataaggtcct ctggatcaac tacccctcct
       61 ccgtccacct cgcctcaatc gagacgatcg aggagacaac gcgccagatc ctacgcgatg
      121 ccgctccggg cgaccggctt ctcgtgggca tcaccgagga tgtgcccgag aaccggtggc
      181 gcgagaactt cctcgcgatc tcgcgggtgc tcgacaccga tggccgaacg cccgtcgcgg
      241 cgtcatgagg cctcgcgcga cgatagccgg cgcccgggcc accgagacct gtccgtattc
      301 agcacactgg taggcctggg gtggtctgaa acaccacccc gggcctcctg ctacttctct
      361 tcttcctcct catactcctc gtaccagccc tccggtcccg cgctggaatg gagatgcgtg
      421 gcgaattcga ggagtgccac cgccgcttcg acgcgagttt cgccgtcttg gctctcgtcg
      481 ttggcgacct ggcggagcac cttcaccgcg tcaccaatcg ctccctggaa ttcctgtgca
      541 tcgagctcga ccatcatctc aagactcctt atttcgtttc cctgccgggg atcacggcgc
      601 ggccgtgatc cggatctccc gggtatcctg aagtggcacc tccagcgcat gggtcaccct
      661 gccggtgacg gtgtagcttc ccggcggcac gcgagcaccg gtattgtccg tcccgtccca
      721 tcgcacggtg aacgcgcgcg tctcgtctgg caacaacacc aactgcgtca ctgcctgggt
      781 gaatgcccgg ccctctgacc agcgccacac cacctcgcca ttgcgctgga cgatgaaatc
      841 gaagagttgg ccggtgcgca tcagcaggcg caccgtctcg ctccccctgt ttcgaatgcg
      901 gagcgtcaac tcgatgggcg cgcccgccgg atactccgct tgcggtgtgc tcaggcccac
      961 ctccaccgac tgttgtattt gcgctgccgg cgttggctgt gccggctcct ccgggctggg
     1021 cggcgcggca ggctgatcca ctgtgcgaat gtagatcgtg cgtgcctgcg gatcccaacg
     1081 cacgtcggcg ccgaacgtct cggccacgac gcgcaatggg accatcgtgc gcccctggat
     1141 cacctgcacc ggcacgcgcg ttcggatgct gcggttgcca gcaacgagcg tctccgcgcc
     1201 cgcctgcacg acaacgcgct gcccctcgta atcgatcacc gcctcgcgcc cggcgggctg
     1261 ccaggtgagg ttcgcgccca gcgcctgcgc gataaaccgg atggggacga gcgtagtccc
     1321 ttggatgatg cgcggggcga tatccgtctg caccatccgc ccgttgacca ccaggcgggg
     1381 agggttctgc gacgctggcg cgcgcgcttg cgatggtgtt tccgcggcag ccgatgcaat
     1441 ccaagtagcc gccgcgagga tcagaaccca actggctgct ctcatgacca caacgttccc
     1501 cctcctaacc acgcgccaaa cggcgcgcgc cttcaacgcg gcatcccggt gtaatgccag
     1561 gacgcgacag ctcgcgggcg cgtttccggc gagagagaga cgcccattgt tgcagggaag
     1621 gaaaggggag ggtgactgcg aagatcgcac ctatttacgc atcgccgagc gcccggcgcc
     1681 cggcctcgcg gaggtagggg atgagcgcct cgaactcctc ccggcgctcc tcgctgaaga
     1741 tgatcacgta gatgctgagg caatcctcgt taaagcagag gaaaatggcg gcatcgagcg
     1801 ggggcagctc gtgcgggctg gcgccctcca gacccgcggc tgaggcgccg ccggcgggga
     1861 aggggatgag gaaggtcgtc ccggagcaga gcgggcatgc cgccagctcc tcgagcgtca
     1921 gctcccacac ctcgcgcgag tagggcgcgc gtatcgcctt gaacgctgtc cattccatcg
     1981 agacccccct ggcgtgatgc accggcacgc gccggcggca tctgcccccc tcgcgcgatt
     2041 ccacggggtc ggcgccgctc cccgcgtgcg tggcgcgcta gctcgaagta ttcaccagaa
     2101 tacttcaaga gaagtctctt gcctggtggc actccgttgt cgccacaagc gcccggcggc
     2161 agagtgtttt cgagggcagc gcccacaaac ctgggccacg cctgagagac ttcttttcat
     2221 ttcattattc tgatgaataa tgcgggctag gcgccccaac gagtacgtct ccactccgag
     2281 agcgatcccg atcaggctcc agaggcagcc catcatgata tcgcccagga tgaggcccag
     2341 gaagaagggg aggagcgttc ggtagagccg cagcccgccc cacttcagga tgctcccctt
     2401 g
//
LOCUS       DAOSCV010000711         2398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28906_length_2398_cov_2.738796, whole genome shotgun sequence.
ACCESSION   DAOSCV010000711 DAOSCV010000000
VERSION     DAOSCV010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28906_length_2398_cov_2.738796"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLY56_15305"
     CDS             complement(<1..570)
                     /locus_tag="PLY56_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22894.1"
                     /translation="MGSRVRHGFTLIELLVVIAIIAILAAILFPVFARARENARKSNC
                     QSNLKQIGTALLQYIQDYNEVYPYGASVNIRDHGNVWDGIQPYIKNTQVFGCASDAQK
                     NCVSLVAGDSRYWGAEWANFKLSYSYNYANGGYSQATFNYVSETAVFAEGIERPYFYQ
                     DGAVLPDGGRGVGYHDAPYRLANRHSEGMN"
     gene            1231..>2398
                     /locus_tag="PLY56_15310"
     CDS             1231..>2398
                     /locus_tag="PLY56_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOJ22895.1"
                     /translation="MRKAIVILTAALALTAIGGLRPARAEKWALLVGINDYEASQIPD
                     LRGCENDVKLMQMVLTQKFGFPQKNIRTVLSRDATKKRILEEFDNWLVKQAKPGDVVL
                     FHFSGHGSQAPDDNGDEPDDRMDETLCPTNIRTSAAFDDIRDEEFGAVINKLKASSIT
                     IILDCCHSGTGTRDLDDLLSPVRVPSDAQGRPMTTRTRMVIRPELLKLADNELPASEL
                     TTAGMFLGDFTRPGAGTRALQESAGTRDAEMNYTLITGCRAEQTSADAPFPMEGNKYY
                     YFGALTYNLVGALMQVDPSDPSWTYERVIAEAVKNLKRKNFKQDPQLEGLAKARVFWA
                     PPVTQTPAPTATQTAQQTPPPPPQPCRPFILVTNVGGDRVELGAGVAAGITAKSV"
BASE COUNT          460 a          776 c          737 g          425 t
ORIGIN      
        1 gttcatgccc tccgagtgcc ggttcgcaag gcggtaaggt gcgtcgtgat agccaacgcc
       61 tcgcccccca tcgggcaaaa ccgccccatc ctggtagaag taagggcgct cgatcccctc
      121 cgcgaagacg gccgtctccg agacgtagtt gaatgtcgcc tgcgagtagc ccccattggc
      181 gtagttgtag gagtagctca gcttgaagtt ggcccactcg gcgccccagt acctgctgtc
      241 tcccgccacg aggctgacgc agttcttctg cgcgtcgctg gcgcagccaa agacttgcgt
      301 gttctttatg tacggctgga tgccgtccca cacgttccca tggtctcgga tgttcaccga
      361 ggccccatag gggtacacct cattgtagtc ctgaatgtac tgcaacagag cggtgccaat
      421 ctgcttgagg ttcgactggc agtttgactt gcgcgcgttc tctctagcac gcgcgaagac
      481 ggggaagagg atcgccgcca agattgcgat gatcgcgatc acaacgagca gctcaataag
      541 cgtgaaaccg tgccggaccc tggatcccat cgatgctctc ctttccgcta acgcagaagg
      601 acagacgggg ggatgcgcgg ccacaaagga gcgacgggtt ggagcgtggc cgcactccct
      661 gtgaatgcca ctattatagc acgcgcctgt acaaatagca agcgcgaaat aagggggctg
      721 gggcttcgag ccacggtagc cggtccggca ttcagcgctg agctcgcgcg agagacgcgt
      781 attcctgctc gataccagca catctgaggg gcaggcgcct cccaagggaa gcgcgcctgc
      841 cgggcagcgc gtgaggaggt gcccctctag tttccacctc gtctgccgat aatctgatta
      901 ggagacggca ggggtaggcc cccctccgtc gggtgttccg aaggctggaa gcgggcctcg
      961 atgcagcatt gccgcggggg gagcatgggc ccctcgcagc gccgtcccgc gtctgcttat
     1021 catgccgctc cctgtcgcgg ttttcagaga gggcagcgac caccttttcc tgatagccct
     1081 gccttcgcaa caccccccgg caaccgacgc ggccatgtgg ccgtttctct cgtcctttgg
     1141 cgcgggttgc tattcgccta caccaagagc tgttgtgccg ggccccgtga tccggcgagg
     1201 gttgagacta gattaagagg aggatggatc atgcgaaagg caatcgtgat cctcaccgcg
     1261 gcgctggcgc ttaccgccat cgggggcctg cgcccggcac gcgcggagaa atgggccctc
     1321 ctggtcggga taaacgatta tgaggcgtca cagatccccg acctgcgagg atgcgagaac
     1381 gatgtcaagt tgatgcagat ggtgctcacc cagaagttcg gcttccccca gaagaatatc
     1441 cggacggttc tgagccgtga tgccaccaag aagcgaatcc ttgaggagtt cgacaactgg
     1501 ctcgtcaagc aggcgaagcc gggcgacgtc gtcctcttcc acttcagcgg ccatggcagt
     1561 caagcgccgg acgacaacgg cgatgagccc gatgaccgca tggatgagac cctctgcccc
     1621 accaacatcc gtaccagcgc cgccttcgat gatattcgcg acgaggagtt cggcgcggtg
     1681 atcaacaagc tgaaggcgag cagcatcacc atcatcctcg attgctgcca ctccggcacc
     1741 ggcacgcgcg atcttgatga tctcctctcg ccggttcgag tgccctcgga tgcgcagggc
     1801 cggccgatga caacgcgcac gcgcatggtg atccgccccg agttgctcaa attggcggat
     1861 aacgagcttc cggcctccga gctaaccacc gccgggatgt tcctgggcga cttcacgcgc
     1921 cccggtgctg gcacccgcgc gctgcaagag agcgccggca cccgcgacgc cgagatgaac
     1981 tacaccctga tcaccgggtg tcgcgccgag cagacatcgg ccgatgcgcc cttcccgatg
     2041 gagggcaaca agtactacta tttcggcgcg ctcacctaca acctggttgg cgccctcatg
     2101 caggtggatc ccagcgatcc ctcctggacc tacgagaggg tgatcgccga ggcggtcaag
     2161 aacctcaagc gcaagaactt caagcaggat ccgcagctgg agggcctcgc gaaagcgcgc
     2221 gtcttctggg cacctcccgt cacgcagacg ccagctccca cagcgacgca gaccgcccag
     2281 cagacgccgc ctccgccgcc gcagccctgc aggccgttca tcctggtcac caatgtggga
     2341 ggcgaccggg tggaactcgg cgcgggcgtg gccgcgggga tcaccgccaa gtcggtct
//
LOCUS       DAOSCV010000712         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_28966_length_2394_cov_3.282599, whole genome shotgun sequence.
ACCESSION   DAOSCV010000712 DAOSCV010000000
VERSION     DAOSCV010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28966_length_2394_cov_3.282599"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PLY56_15315"
     CDS             <1..1301
                     /locus_tag="PLY56_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ22896.1"
                     /translation="IHAGPYESNAFWITLLVGADAHPGTHEVRVTLTPEGETPTTLTA
                     TLTIHPALLPARRDFPVTHWFYADALMDYYKVEPWEEGLWRILDAYLADVVAHGQDTI
                     YVPVFTPPLDGVKRPTQLLDVRQEGERYLFDWRDVRRWIAAAKSHGLKRFEWTHLFTQ
                     WGVQHAIRIYEGQGRERKLLWDPETGATSQTYRDFLAQFLPEFERFLTVEGLMESSFF
                     HLSDEPHGEEHLANYRAARELIRELAPWMRVMDALSEISFARVGLTDTPIPSISRAPE
                     FVAEGFPAWAYFCCGPRGRFLNRLLDTPLVKVRMTGWLLYRLRARGFLHWGYNYWYKS
                     QTQELIDPYTVTDGLAWPGWAHGDPFMVYPGKEGPVDSLRWEVFAESLQDYALLQAAG
                     LDPDHPLLFDLKDYANFPRETAWISRRRADLLARLDARQA"
     gene            complement(1343..>2394)
                     /locus_tag="PLY56_15320"
     CDS             complement(1343..>2394)
                     /locus_tag="PLY56_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ22897.1"
                     /translation="SRSSGPSGSVSTSTGMQASVSLRQLLFDRNHSRDLVRQAEAQED
                     AAERTLARTEADLVLQVKQAFYTYVQNMRLVQIHETNLRNQQQHLAQAQARLQAGVGL
                     PLDVVRAEAAVSEATLNLQVARNNASTARVSLALLMGIDPRTPIEVAESEEPAPPAED
                     LESLVQLAIAQRPELGQAEATRAAAIHALDAARSTDAPTVAATAGVAARGVSTPPLNH
                     FLSVGIQVQWSPFDGGVKDGRVQEAQASIQAAEAQLEGTRRTIIADVSQAYLNLRTAE
                     QRVVTADAGIANAEEALRLATGRYNAGLGTFLDVLDAQAALLTARTQRVNAEVAVAQA
                     RAALARAIGTPLDEE"
BASE COUNT          357 a          804 c          811 g          422 t
ORIGIN      
        1 cgatccacgc cggcccctat gagtcgaacg ccttctggat caccctcctg gtgggtgcgg
       61 acgcccatcc aggaacgcat gaggtgcgcg tgaccctcac gccggagggt gagacgccca
      121 ccacgctcac ggcgacgctc acgatccacc cggcgttgct tccggcgcga cgcgattttc
      181 cggtgacgca ctggttctat gccgacgcgc tgatggatta ctacaaggtg gagccgtggg
      241 aggaaggcct ctggcggatc ctcgacgcgt acctcgccga cgtggtggcg catggccagg
      301 atacgatcta cgtgccggtc ttcacgcccc cgctcgatgg ggtgaagcgc ccgacgcagc
      361 tcctcgatgt gcgccaggag ggagagcgct acctcttcga ctggcgcgat gtgcgccgtt
      421 ggatcgccgc cgcgaagtca cacggcctga agcgcttcga atggacccac ctcttcacgc
      481 agtggggcgt gcaacatgcg atccgcatct atgagggcca ggggcgcgag cggaagcttc
      541 tgtgggatcc ggagacaggg gccacttcgc agacgtaccg cgacttcctg gcgcaattcc
      601 tccccgagtt cgagcgtttt ctgacggtgg aggggctgat ggagagcagc ttcttccacc
      661 tctccgacga gccgcacggc gaggagcacc tggcgaacta ccgcgcggca cgcgagctca
      721 tacgcgagtt ggctccctgg atgcgcgtga tggacgcgtt gagcgagatc agcttcgcgc
      781 gagttggcct caccgacacg ccgatcccct ccatctcgcg ggcgcccgag tttgtcgccg
      841 agggcttccc ggcgtgggcc tacttctgct gtggcccgcg cggccgcttc ctgaaccgct
      901 tgcttgatac ccccctggtg aaggtgcgca tgaccggctg gctcctctac cgcctgcgcg
      961 cgcgtggctt cctgcactgg ggctacaact actggtacaa gagccagacg caggagctga
     1021 tcgaccccta caccgtgacc gatggcctgg cctggcccgg ttgggcgcat ggagacccct
     1081 ttatggtcta ccccgggaag gagggtccgg tcgactccct gcggtgggag gtcttcgccg
     1141 aaagccttca agactacgcg cttctgcagg cagcggggct ggatccggac cacccgcttc
     1201 tctttgacct gaaggattac gccaacttcc cacgcgaaac cgcctggatc agtcgccggc
     1261 gcgccgacct gctggcccgg ctggatgcca ggcaggcgta ggtgttatct tgcgcgtgct
     1321 gcccgcgccc ggccagggcg ggctactcct cgtccagagg ggtgccaatg gcgcgggcaa
     1381 gggcggcacg cgcctgcgcg actgccacct cggcgttgac gcgctgggtg cgcgccgtca
     1441 gcagggccgc ctgggcatcg agaacatcga ggaaggtacc cagaccggcg ttgtagcgcc
     1501 cggttgccag gcgcagagcc tcttccgcgt tggcgatccc cgcgtcggca gtcaccacgc
     1561 gctgctccgc ggtgcgcagg ttgaggtagg cctgcgaaac atccgcgatg atggtgcgcc
     1621 gcgtgccctc aagctgcgcc tcggctgcct gaatgctggc ctgggcctct tggacgcggc
     1681 catccttcac cccgccatcg aaggggctcc actgcacctg gatccccacg ctcaggaagt
     1741 ggttcagcgg tggagtgctg accccgcgcg ccgcgacgcc agcagtggcg gccaccgtgg
     1801 gcgcgtcggt gctcctggct gcatccaggg cgtggatggc ggctgcgcgc gtggcttcgg
     1861 cctggccgag ctccggccgt tgggctattg ccagttgcac cagactctcc agatcttccg
     1921 ccggcggagc cggctcctcg ctctctgcca cttcaatcgg cgttcgcggg tcgatgccca
     1981 tcagcagcgc cagggacacg cgcgccgttg aggcgttgtt gcgggcgacc tggaggttca
     2041 gcgtcgcctc ggagacagcc gcctcggcgc ggacgacatc caggggcagg ccaacgcccg
     2101 cttgcagccg ggcctgcgcc tgtgccaggt gctgctgctg gttacgcagg ttcgtctcgt
     2161 gaatctgaac caggcgcatg ttttgaacat aggtgtagaa cgcctgcttc acctgaagca
     2221 cgagatcggc ctcggtgcgc gccagagttc gctcggccgc gtcttcctga gcctccgcct
     2281 ggcgcaccag gtcgcgcgag tggttgcggt cgaaaaggag ctggcgcaga ctgacggagg
     2341 cctgcatccc ggtggaggtg gagaccgatc cggatgggcc gctgctccgg gatg
//
LOCUS       DAOSCV010000713         2388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29075_length_2388_cov_3.703815, whole genome shotgun sequence.
ACCESSION   DAOSCV010000713 DAOSCV010000000
VERSION     DAOSCV010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29075_length_2388_cov_3.703815"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1443
                     /locus_tag="PLY56_15325"
     CDS             100..1443
                     /locus_tag="PLY56_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ22898.1"
                     /translation="MGRLNILYIHSHDTGRYVQPYGYAVETPRIQQLAEEGVLFRQAF
                     CANPTCSASRSVLLTGRYAHSNGMIGLAHRGSRLNDYRQHLASFLSRNGYETALSGMQ
                     HEAADRQLLGYERFLDDGPGGNTQEGVARRAAAFLAERHDRPFFLSCGFSLTHRTGRG
                     IQWHNGEASPLGDPRYCRPPAPLPDLPEIRRDFADFRASVRRLDECMGIVFDALEESG
                     LAEETLVVCTTDHGIAFPFMKCNLTDHGTGVMLILRGPGGFTGGKVVDALVSHVDIFP
                     TLCQMAGIPQPDWLQGTSLMPLVTGDAESVRDEVFAEVNYHAAREPMRSVRTARYRYI
                     RRYEVHPHPVLPNCDESVSKRELLRYGWRERPQQEEALYDLIFDPNEACNVAKDPLYA
                     DALAEMRARLNRWMRETEDPLLTGRMEPWPGMASNPVDGNSPQEEAGPAEPWVAR"
     gene            1535..2026
                     /locus_tag="PLY56_15330"
     CDS             1535..2026
                     /locus_tag="PLY56_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22899.1"
                     /translation="MEGLGQWFEQVDRRVVAWFQRHSVPALRVALGIVFVWFGGLKVA
                     GVSPVADLVATTVAWLPAGWFVPFLGVWEVAIGVGLLFRLFLRLTLLLLAAQLAGTFL
                     VLLVRPDLAFAGSPLLLTLIGEFVVKNLVLLAAGMVLASTVRDEDETCLATGVRGSGA
                     RRR"
BASE COUNT          396 a          706 c          817 g          469 t
ORIGIN      
        1 cgtggcgcgt tcgtccggcg tgcgggctat cgcgccggcg ggagaacggc ggcattcccc
       61 gcgttttgac ttcggaacat cgcagggaag gggagaggca tgggtcgcct caacattctg
      121 tatattcact cgcatgacac cgggcgctat gtgcagccgt acggctatgc cgtggagacc
      181 ccgcgtatcc agcagctcgc ggaagaggga gttctcttcc gccaggcatt ttgcgccaac
      241 ccgacctgtt ccgccagtcg ctcggttctg ctcacggggc gatatgctca ctccaatggg
      301 atgattggtc tggctcaccg cggctcgcgc ctgaacgact atcgccagca tctggcgagc
      361 ttcctgagcc ggaacggcta cgagacggcg ctgtccggga tgcagcacga ggctgccgac
      421 cggcagctcc ttggctacga gcgtttcctt gatgacggcc ccggggggaa tacccaggag
      481 ggggtggccc gccgggcagc cgcgtttctt gccgaacggc atgaccggcc tttcttcctc
      541 tcctgcggct tttcattgac gcaccgcacg ggcagaggca tccaatggca caacggcgag
      601 gcctcgcctc tgggcgatcc tcgctactgt cggccgcccg cgccgctccc ggatcttccc
      661 gagatacgtc gcgactttgc cgacttccgc gccagcgtcc ggcggcttga cgagtgcatg
      721 gggattgtct tcgatgccct cgaggagagc ggtcttgccg aagagacgct ggtcgtctgt
      781 accaccgacc acgggatcgc tttccccttc atgaagtgca atttgactga tcacggcacc
      841 ggcgtcatgc tgatcctgcg cggcccgggc gggtttaccg ggggtaaggt ggtggatgcc
      901 ctcgtcagcc acgtggacat cttcccgact ctgtgccaga tggctgggat tccgcagccc
      961 gactggcttc agggcacgtc tttgatgccg ctcgtcacag gcgatgccga gtcggtgcgg
     1021 gatgaggtct tcgctgaggt gaactaccac gcggcaaggg agccgatgcg cagcgtgcgg
     1081 acggcacgct accgctacat ccggcgctac gaggttcatc cgcatccggt cctccccaac
     1141 tgcgatgaga gtgtgagtaa gcgagagttg ctccgttacg gctggcgcga gcgccctcag
     1201 caggaggaag cactctacga cctcatcttc gacccaaatg aagcgtgcaa cgtagcgaag
     1261 gacccactgt atgcggacgc gctggcagag atgcgcgcgc gattgaaccg ctggatgcgc
     1321 gagacggagg atcccctgtt gactgggcgg atggagccgt ggccggggat ggcgtcgaac
     1381 cccgtcgatg gaaactcgcc gcaggaagag gcgggcccag cagaaccatg ggttgcgcga
     1441 taatccgcgg gcggccggcg cgcaggctcc ggcaaggtgg catgtaggca acggcgactc
     1501 catggggtaa gagaccctcg tcagggggaa cgccatggag ggactcggcc agtggtttga
     1561 gcaggtggac cggcgggtcg tggcctggtt tcagcgccac agcgtgccgg ctctgcgtgt
     1621 tgcccttggg atcgtcttcg tgtggttcgg cggcctgaag gttgccggcg tcagccccgt
     1681 cgccgatctg gtggcaacca ccgtcgcgtg gctccctgcc ggttggtttg tgccattcct
     1741 cggcgtgtgg gaggtcgcga ttggggtcgg gctcctcttt cgcctgttcc tgcgcttgac
     1801 gctcctgctc ctggccgcgc aactggccgg cactttcttg gtgctcctcg tccggccaga
     1861 tctggctttc gcggggtcgc ccctccttct gacgctcatc ggcgagttcg tggtgaaaaa
     1921 cctggtgctg ctcgccgcgg gaatggtact cgcgagtact gtgcgcgatg aggatgagac
     1981 gtgccttgcg acgggcgtaa gggggagcgg ggcgcgccgt cgatgatcga atgcctttat
     2041 caatggatgg tggtggggaa cgcccacccg gatcctcgcg cggcgttccg cgacgatcga
     2101 gcaatggagc tgggcgcgac acggacgtct tagcgaagag gctgccggcg actaatggta
     2161 acgtaacgca aggtatacgc attacatgct ccaggctatg agatcatgtg ggagcccacg
     2221 tcggcgagga cgagcccgca cagatggatg gaggtacttt gtagtgccta tggagagagt
     2281 gagacgatct ttatggccat aggccaaacg gcaatttggt ggttgacact cgggatgggt
     2341 tctggtaaga taatagccgt cacgagggag tgcctggggt ttcgacag
//
LOCUS       DAOSCV010000714         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29097_length_2387_cov_2.942539, whole genome shotgun sequence.
ACCESSION   DAOSCV010000714 DAOSCV010000000
VERSION     DAOSCV010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29097_length_2387_cov_2.942539"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="PLY56_15335"
     CDS             <1..1894
                     /locus_tag="PLY56_15335"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF021919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ22900.1"
                     /translation="RPEVAGAPALVFAAALEPQPAEREGWGEVLLRVARGGFNAVYVS
                     VPWNVGKEGSLAVADLDAFLAKVAETGLKAIIGPPIDDPFGLWREWLAPIQPVLEKHA
                     ETILWLQARADSPQGGAAELSKAAQALKCALPVAEGVTTQVTSERDPQRPYREPEVSV
                     ALRVALAEGATALVWDQPFAGTAPPLISIDDVPPAVVHHGRGGALPIAYYEAHLFSQV
                     ARCLGDPLAKAQPAEGAAADAAGISVTQRNLGRQGFLFVRAPAEPVQRFHLSYTDPES
                     GEKMTVPRTQGLSLRSFGAGVRILPLNLPVPGATIRYSTCEVYGVYQVGERTVILVTD
                     DDGGLNEIALKLEGAEKPAIRNAPIEPVWDAASRTLTVSVVPSFAESFMVIGEKLVLG
                     VVPTERAQRTWSVRYGDGTVPMITSAYLVGQDATLEGKIGLPVFLQQGRSAVSMVLPK
                     EPRYLAVDSRGAKTAFDATTGVLSFFLQSPVLLGVETKTPIVQVLDRARHKAGLAAET
                     AENAQPDLDDSKWPEVRLDKAKTAGWYRIGFSVPALENWILHWKATVEVTGKAVVYLN
                     GEPIGTAASTDEKGGFVDLDLPSSLIRSKNTLAFAVDANSRVRKVGISPHVEDTVQRQ
                     LLIFFP"
     gene            2069..>2387
                     /locus_tag="PLY56_15340"
     CDS             2069..>2387
                     /locus_tag="PLY56_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22901.1"
                     /translation="MGVQSAECREWAGLGLRRALSAARWATVALALGLALLIPAPARA
                     DTGFEAGFIGRLGSFLVPGGYFFTSDAARDALDDVLWYHEADFIRYGLGVGVAGVAFT
                     LKGI"
BASE COUNT          362 a          757 c          848 g          420 t
ORIGIN      
        1 gcgcccggag gtggcgggcg cgccggcatt ggtcttcgcg gcggcgctcg aaccccagcc
       61 cgcagaacgt gaggggtggg gcgaggtgct gctgcgcgtc gcgcggggcg gcttcaacgc
      121 cgtttatgtc agtgtgccgt ggaatgtggg caaagaggga agcctcgcgg tcgctgacct
      181 cgatgcgttc ctcgcgaagg tggccgaaac ggggctgaag gcgatcatcg ggcctcccat
      241 tgacgaccca tttggtctct ggcgcgagtg gctggcaccg attcagcccg tgctcgagaa
      301 gcacgctgag acgatcctgt ggctccaggc gcgcgccgat tcgccccagg ggggcgcggc
      361 cgagctttcc aaggcggcgc aggcgctgaa gtgcgctctc ccggtagccg aaggcgtgac
      421 tactcaggtg acgagcgagc gcgaccccca gcgcccctat cgggagccgg aggtcagcgt
      481 tgccctgcgc gtcgcactgg ccgagggcgc gaccgcgttg gtctgggacc agccttttgc
      541 cggcaccgcg ccgcctctca tctccatcga tgacgtgccg cctgccgtcg tgcatcacgg
      601 gcgtgggggc gcgctcccga ttgcctacta tgaagcccat ctcttcagcc aggtggctcg
      661 ctgcctgggc gacccgttgg cgaaggccca gcccgctgaa ggcgccgccg ccgatgcggc
      721 gggcatctcg gtgacccagc ggaatctggg gcgccagggt ttcctcttcg tccgcgcccc
      781 ggcggagccc gttcagcgct tccacctttc ctatacggat ccagaatcgg gcgaaaagat
      841 gaccgtgccc cgaacccagg ggctttcgct gcgtagcttt ggcgccggcg tgcgtattct
      901 tcccctgaac ctgccggttc ctggcgcgac catccgttac agcacctgcg aggtatatgg
      961 ggtttaccag gtgggcgagc ggacggtgat cctggtgacc gacgatgacg gcgggctgaa
     1021 cgagattgcg ctgaagctgg aaggcgcgga gaaaccggcg atccggaatg cccccatcga
     1081 gcccgtctgg gacgcggcaa gccgcaccct gaccgtgagc gtggtgccct ccttcgccga
     1141 gagcttcatg gtcatcggcg agaagctggt cctcggagtg gtgccgacgg agcgcgccca
     1201 gcgcacctgg agcgtccgct atggcgatgg caccgtgccg atgatcacca gcgcctacct
     1261 ggtgggccag gatgccacgc tggaagggaa gatcggcctg cccgtcttcc tgcagcaggg
     1321 ccggtcggcg gtttccatgg tgttgccgaa ggagccgcgc taccttgccg tggacagccg
     1381 gggagcgaaa acggcgtttg atgccacgac cggcgtgctc tccttcttcc tccagtcgcc
     1441 cgttctcctc ggtgtggaga ccaagacgcc catcgtccag gtgctcgacc gcgcgcggca
     1501 caaggcgggg ctggcggcgg agaccgccga gaacgcgcaa ccggacctgg acgattccaa
     1561 gtggccggag gtgcgcctgg acaaggcgaa aaccgcgggc tggtaccgca ttggcttctc
     1621 cgtgccggcg ctggagaatt ggatcctgca ctggaaggcg accgtggagg tgaccggcaa
     1681 ggcagtggtg tacctgaatg gcgagccgat cggaaccgcc gccagcacgg atgagaaggg
     1741 cgggtttgtc gatctcgacc tcccatcctc gctcatccgt tcgaagaaca cgctggcgtt
     1801 cgccgtggat gccaactcgc gcgtgcgcaa ggtagggatc tcgccgcatg tggaagacac
     1861 cgttcagcgg cagcttctga tcttcttccc ctaacgccac gcagatcgtg tgacgggccc
     1921 ggctcctgtt cgagtgcgcc cagcaaggag ggcgccggct ggaaccgggc ccgtggcttg
     1981 tacccggctt ctcgtttggc ccgtgctcct ggcgggtatc tctcttctag gccgcgaggg
     2041 tgttgcggcg gcagggaagg agaagcgcat gggagtgcag agtgcggagt gcagagagtg
     2101 ggcaggcctc gggttgcgcc gggcgctatc cgccgcgcga tgggcgacgg tcgcgctggc
     2161 gctcggtctg gcgcttctga tcccggcgcc agcgcgcgcc gatacggggt ttgaagccgg
     2221 gttcatcggc cggctgggca gcttcctggt gcccgggggc tacttcttca catccgatgc
     2281 cgcccgtgat gccctggatg acgtgctatg gtaccatgag gcagatttca tccgctacgg
     2341 tttgggagta ggcgtcgccg gcgtggcctt cactctgaag gggatca
//
LOCUS       DAOSCV010000715         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29157_length_2383_cov_4.009021, whole genome shotgun sequence.
ACCESSION   DAOSCV010000715 DAOSCV010000000
VERSION     DAOSCV010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29157_length_2383_cov_4.009021"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="PLY56_15345"
     CDS             <1..1965
                     /locus_tag="PLY56_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ22902.1"
                     /translation="LAREDKLDPVVGRHKEIERVIQILSRRTKNNPCLIGEPGVGKTA
                     VAEGLAQRIIRGDIPEPLKDKRVVSLDLAGLVAGTKYRGEFEERMKRVMEEIRRAQGE
                     IILFVDELHTLVGAGAAEGAIDASNILKPALARGELQCIGATTMDEYRKYIERDGALA
                     RRFQQVKVDEPSIEETEEILKGLRQRYEQHHQVRIGDDAVAAAARLSSRYITDRFLPD
                     KAIDVIDEASSRVRLRSALPPAEIRTAKQELEDLQRQKEEATKNYEYEEVAQIRERVQ
                     RLQTRIAELEEEWRQRQAEAKLLVDEEEVAQIIASWTGVPVTRLVEAETAKLLRMEEQ
                     LHKRIIGQDEPVNAVARVIRRARSGLKDPRRPTGSFIFLGPTGVGKTELARALAEFLF
                     DDETAMVRIDMSEYMERFAVSRLVGAPPGYVGYDEGGQLTEAVRRRPYTVVLLDEIEK
                     AHPEVFNILLQVMEDGRLTDSQGRVVDFRNAVIIMTSNVGAQMIQEDRMGLRRTPEKQ
                     EPDEKQYEAMKKRVMEELKKTFRPEFLNRVDETVFFHALTSYEIRQIVDLMLSRVAEQ
                     LRQHGIELETSESARDLLAREGFDPTYGARPLRRAIQRLVEDPLSEDILLGTFHRGDV
                     IRLEAEGDKITFHKVSLDAADAEKEAALPI"
     gene            2076..>2383
                     /locus_tag="PLY56_15350"
     CDS             2076..>2383
                     /locus_tag="PLY56_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ22903.1"
                     /translation="MPKAYTKFVCSACGHEAPRWMGRCPDCGEWNSLVEEMVRPASPG
                     SRSAAAGGRGLTTARSQPIGAIDREEHPRRMTGIGEFDRVLGGGVVPGSLILVGGDPG
                     "
BASE COUNT          434 a          736 c          839 g          374 t
ORIGIN      
        1 ctggctcgcg aggataagct cgatccggtg gtcgggcgcc acaaggagat cgagcgcgtg
       61 atccagatcc tgagccggcg cacgaagaac aacccgtgcc tgatcggcga gccgggcgtc
      121 ggcaagacgg ccgtcgccga gggtctggcc cagcggatca tccgtggcga catcccggag
      181 ccgctgaagg acaagcgcgt cgtctcgcta gatctggccg gcctggtcgc cggcacgaag
      241 tatcgtggcg agttcgagga gcgcatgaag cgcgtgatgg aggagatccg gcgcgctcag
      301 ggcgagatca tcctctttgt ggatgagctg cacaccttgg tgggcgccgg cgccgccgag
      361 ggcgccatcg acgcctcgaa cattctcaag ccagcgttgg cgcgcggcga gctgcagtgc
      421 atcggcgcga ccacgatgga tgagtaccgc aagtacatcg agcgcgatgg cgcgctggcc
      481 cgccgcttcc agcaggtgaa ggtcgatgag ccctcaatcg aggagaccga ggagatcctc
      541 aagggcctgc gccagcgcta cgagcagcac catcaggtgc gcatcggcga tgacgccgtg
      601 gcggccgccg cgcggctttc gagccgctat atcacggacc gcttcctgcc ggataaggca
      661 atcgacgtga tcgacgaggc atcgtcgcgc gtgcgcctgc gctcagcgct cccgccggcg
      721 gagatccgca cggccaagca ggagctggag gacctccagc gacagaagga agaggccacc
      781 aagaactacg agtacgaaga ggtggcccag attcgggagc gtgtccagcg tctccagacg
      841 cgcatcgccg agctggaaga ggagtggcgc cagcgccagg ccgaagccaa gctcctcgtg
      901 gatgaggaag aggtggccca gatcatcgcc tcgtggactg gcgtgcccgt aacacgcctg
      961 gtggaggcgg agacggcgaa gctcctgcgc atggaggagc agctccacaa gcgcatcatc
     1021 ggccaggacg agccggtgaa cgcagtggca cgcgtgattc gccgggcgcg ctcgggcctc
     1081 aaggatccgc gccgccccac gggctcgttc atcttcctcg gccccaccgg cgtcgggaag
     1141 acggagctgg cgcgcgcgct cgcggagttc ctcttcgatg acgagaccgc gatggtgcgc
     1201 atcgacatgt ctgagtacat ggagcgcttc gccgtgtcgc gactggttgg tgcgcctccg
     1261 ggctatgtcg ggtatgatga gggaggccag ctcaccgagg cggtgcgccg gcggccctac
     1321 acggtggtct tgctcgacga gatcgagaaa gcccaccccg aggtgttcaa catcctcctc
     1381 caggtgatgg aggatggccg gctcactgat agccagggtc gcgtcgtgga cttccgcaac
     1441 gccgtgatca tcatgacgtc gaacgtcggc gcccagatga tccaggaaga ccggatgggc
     1501 ctgcgccgca ctcccgagaa gcaggagccg gatgagaagc agtacgaggc gatgaagaag
     1561 cgcgtgatgg aagagctgaa gaagaccttc cggccggagt tcttgaaccg cgtcgatgag
     1621 acggtcttct tccacgcgct gacgtcgtac gagatccggc agatcgtgga tctgatgctc
     1681 tcgcgcgtgg ccgagcagct ccggcagcac ggcatcgagc tggagacaag cgagagtgcg
     1741 cgtgacctgc tcgcgcggga gggcttcgac ccgacctacg gagcgcggcc gctgcggcgc
     1801 gcgatccagc gcctggtcga ggatccgctc tccgaggata ttctcctcgg caccttccac
     1861 cggggcgatg tgattcgcct cgaagccgaa ggcgataaga tcaccttcca taaggtgagc
     1921 ctggacgcgg cagatgccga aaaggaggca gcgctgccga tctagcgacc gccttcttca
     1981 aggcgcgagt gatcgcgctg taggtgcgcg ggggtgcggc gatatcgaat ccggtagagc
     2041 cgggcccccg cttttttcat ggatggagtc tttgcgtgcc gaaagcctac acgaagttcg
     2101 tgtgctccgc ctgtggccat gaagcgccca gatggatggg ccgatgcccc gactgtgggg
     2161 agtggaacag cctggtggag gagatggtgc ggcccgcatc gcctggctct cgctctgccg
     2221 ccgctggagg aaggggtctc accacggcgc gctcgcagcc catcggcgct atcgatcgcg
     2281 aggagcaccc gcgccgcatg accggcatcg gcgagtttga ccgcgtgctt gggggaggcg
     2341 tcgtgcccgg ctccctcatc ctggttggtg gcgaccccgg cat
//
LOCUS       DAOSCV010000716         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29173_length_2382_cov_2.958315, whole genome shotgun sequence.
ACCESSION   DAOSCV010000716 DAOSCV010000000
VERSION     DAOSCV010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29173_length_2382_cov_2.958315"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..913
                     /locus_tag="PLY56_15355"
     CDS             119..913
                     /locus_tag="PLY56_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOJ22904.1"
                     /translation="MPERLYRQEDAAGSENDPEGATGQSGGNATAPGKGPRMILYIVT
                     EKREKYERNGDHAQKLKLEKISKQPCLVQFYEDVTLEMVKRLGVQAVVFSGYSTPLQE
                     HRLESFQGIYELVRQGDVPMLGLCGGHQLICELWAEHNDQGLTRMEGYPIRKLRPGEP
                     DHHAAYHPGWFKEWGFYPIRIVRQDPLFDGLEDGFMACEYHQKEVKELPRDFVLLAST
                     EAVRIQAVRHVSRPIYGTQFHPENYTDYYPAGRRILENFFRIAGVL"
     gene            1024..2145
                     /locus_tag="PLY56_15360"
     CDS             1024..2145
                     /locus_tag="PLY56_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOJ22905.1"
                     /translation="MKDEASDAPLTYAASGVDYDLMDPFKRMAQQAARATAGNLAHFG
                     FQEVAASRGESAYLIEAPDAYLAHVEEGLGTKNLVADAMYRLTGRTFYDHIGQDTVAM
                     IVNDLITVGAMPVSIAMHLAVGTSEWFADTQRVADLVRGFQHACNLSRAVWGGGETPT
                     LRKIVAPDTAVLAGSAMGIIRPKERLIRANLAEGDVILFLAASGIHANGLTLAQRIAE
                     RLPQGYLTPLPDGRPYGEALLDPTPIYVPVLDDLLSAGIVPHYTANVTGHGWRKLMRA
                     SEPFVYVIDRIPEPPPIFPFMQEHGPITDEEAYGNLNMGAGFALYLAPEHVEQAQAIA
                     ESHGIGTLVAGTVEKRGDDKRVVIRPKGIEYAGETLGVR"
BASE COUNT          440 a          772 c          760 g          410 t
ORIGIN      
        1 caaccgcagg atcgcctcgt ggtggtcgtc tcgtgcccga agggatcgcc aaatccagga
       61 tatccgcact cccatccgcg cccatctcct tcatccgtgg gattgatgct ggcccgggat
      121 gccagaacgg ctctaccggc aggaagacgc ggccggctcg gaaaacgatc cggagggcgc
      181 gactggccag agcggtggaa acgccactgc gcccggaaag ggaccgcgca tgattctcta
      241 catcgtcacc gagaagaggg agaagtacga gcggaatggg gaccatgccc agaagctcaa
      301 gctggagaag atcagcaagc agccttgcct ggtgcagttc tacgaggatg tcaccctgga
      361 gatggtgaag cgccttgggg tccaagccgt cgtcttcagc ggctactcga cccctctgca
      421 ggagcaccgc ctggagagct tccagggcat ttacgagttg gttcgccagg gcgacgtgcc
      481 gatgctcggg ctctgcggcg ggcaccagct gatctgcgag ctgtgggccg agcacaacga
      541 ccagggcctc acccgcatgg agggctaccc gatccgcaag ctgcgccccg gagagccgga
      601 tcaccacgcg gcgtatcacc ccggctggtt taaggagtgg ggcttctatc ctatccgtat
      661 cgtgcgccaa gatcccctgt tcgatggtct tgaagacggc ttcatggcgt gcgagtacca
      721 ccagaaggag gtaaaagagc ttccgcgcga ctttgtgctc ctggccagca cggaggcagt
      781 gcgtatccag gcagttcgcc acgtctcccg ccccatctat ggcacccaat tccatcccga
      841 gaactatacc gattactatc cggcgggccg gcgcatcctg gagaacttct tccgtattgc
      901 gggagttctg taacctggaa gccgagagca cgccgcggga gcccgtgcct gacggtctag
      961 cgcccgagcc gcagggagag cggcggtgat gccgaatctg agagtggagg agggaaaccc
     1021 cgaatgaagg atgaggcttc ggatgcgccg ctcacctatg cggctagcgg tgtcgactat
     1081 gacctgatgg atccgttcaa gcggatggct cagcaggctg ctcgggctac cgccggcaac
     1141 ctggcgcact tcgggttcca ggaagtggcg gccagccgtg gagagagcgc ttacctgatc
     1201 gaggcgcctg atgcctacct cgcgcatgtc gaggagggcc tgggcaccaa gaacctggtc
     1261 gccgacgcca tgtaccgcct caccgggcgt accttctatg atcatattgg ccaggacacg
     1321 gtggcgatga tcgtcaacga cctgatcacc gtgggcgcca tgccggtctc catcgccatg
     1381 catctcgctg tcggcacctc tgagtggttt gctgatacgc agcgcgttgc cgacctggtg
     1441 cgaggcttcc aacacgcgtg caacctctcg cgtgccgtct ggggaggcgg cgagacgccg
     1501 actctgcgga agatcgtcgc gccggacaca gccgtcctgg ccggatccgc gatggggatc
     1561 attcgtccga aggagcgcct gatccgcgct aacctcgcgg agggcgacgt gatcctcttc
     1621 ctcgccgcat ccggcatcca cgccaatggc ctcaccctag cgcagcggat tgccgagcgg
     1681 cttccccagg gctacctgac ccccctcccg gatggccggc catatggcga ggcactcctc
     1741 gatccaaccc ccatctatgt gcccgtgctc gatgacctcc tctcggcggg catcgtgccc
     1801 cactacacgg ccaacgtcac cggccacggg tggcgcaagc tgatgcgcgc cagcgagccg
     1861 ttcgtttacg tcatcgaccg catcccggag ccgccgccga tctttccttt catgcaggag
     1921 cacggcccga tcaccgatga ggaggcctac ggcaatctca acatgggcgc tggtttcgcg
     1981 ctctacctgg ctcccgagca cgtggagcag gcccaggcga ttgccgagag ccacggcatc
     2041 ggcacgcttg tcgccggcac cgtcgagaag cgcggcgatg ataagcgcgt tgtcatccgc
     2101 cccaagggga ttgaatacgc aggcgagacg ctgggggtgc gctagaggga gtgcttcggc
     2161 cctgctgcgc gttacgcagg ccacctgttc agctggcctg cccgcggcca ggggacgcca
     2221 tcgccttcga tagactggtt ggggtaggag atccagagtg aatggcgaac tccgctcata
     2281 agagttcgcc acagacccct ggtcggacgg cacagccggc gtgtgcgagc gctagcgtcg
     2341 aggggaaccg cgcaacgtct attcccaaga cgcccccgcg ac
//
LOCUS       DAOSCV010000717         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29229_length_2378_cov_3.047353, whole genome shotgun sequence.
ACCESSION   DAOSCV010000717 DAOSCV010000000
VERSION     DAOSCV010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29229_length_2378_cov_3.047353"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLY56_15365"
     CDS             complement(<1..288)
                     /locus_tag="PLY56_15365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ22906.1"
                     /translation="MPDNLVWGRALVAAMHALPPECTAVALVRHAEREPIPSFQENDV
                     AALTPRGEADAYQLGRHLPAGRPIRLFHSPIGRCQMTAVRIAEGFRSVGGSA"
     gene            complement(492..668)
                     /locus_tag="PLY56_15370"
     CDS             complement(492..668)
                     /locus_tag="PLY56_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22907.1"
                     /translation="MICDTCGRESERVARVVIDQGYNRLLAKPLWNCPECFEKKEQER
                     RKRKEREATAQAAA"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..>2378
                     /locus_tag="PLY56_15375"
     CDS             <1407..>2378
                     /locus_tag="PLY56_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ22908.1"
                     /translation="HLGRTFSMVAAMLLCGFAAGRWGKRWTLGTSAVLMGAGVLFCAL
                     APGYGVLLFALTLAGLGEGVIEGLATPFVQDLHTDEPGRYINFAHSFWSIGVLVTVLL
                     SGALITYGVSWRVVIGAVALLGFFTALLLLLPARKGHAYPERPEPLHWQTVWAQALAI
                     FRAPTFWLFFAAMFVAGGGEFCLTFWCASYIQLSFMDAAWAGGVGTACFSAGMFLSRA
                     GWGYLVRQDQLKHLIIWSALGGTLVTLLFPHLTNLWAFFALLVAAGVAPRRVLLIGGA
                     AANPAVAAVAATVFAVPIRIPAPGEYVADGAARQAAWALAGDLPDWS"
BASE COUNT          332 a          802 c          783 g          451 t
ORIGIN      
        1 ggcggaaccg cccacggagc ggaagccctc ggcgatgcga accgccgtca tctggcagcg
       61 tccgataggg ctatggaata gccggatcgg gcggcctgct ggcagatggc gtcccagctg
      121 gtaggcatcc gcctctcctc ggggtgtcag tgctgcgaca tcattttcct ggaagctggg
      181 gatcggctcg cgttccgcgt gccgcaccaa cgcgacagcg gtacactctg gtggcagcgc
      241 gtgcatcgct gccaccaagg cacggcccca cacgaggttg tcaggcatcg gcttctgtct
      301 ctcgctcctc cgaaggagag ccggagacga acggaacgag ttcggcttcg cccccctcgg
      361 gcaggctcat ctccggctca actatcaggg caacgcaact ccccggtccc gggatccgtg
      421 tcggaagcac ggggatgcta gtggcgcgca ttatcgatcg ctcgcccgcg aggccctggg
      481 gcaggttcag ttcaggcggc tgcctgggcg gtcgcctcgc gctccttgcg cttgcgccgc
      541 tcctgctcct tcttctcgaa gcactcgggg cagttccaga gaggcttcgc caacaagcgg
      601 ttatacccct gatcaatcac cacgcgcgcc acgcgctcgc tctctcgccc gcacgtgtcg
      661 caaatcatgc gctctttcct ttccacggtc aggtggtgcc gccagaggat gctggcgggc
      721 accacagcgt ctatcccacc gccggatgta gaccatccgg acggaatgga gattcaatca
      781 ggttcccatc agggaggagg gcaggcgcct ttgtctccgg ctccggcaac cgcggagccg
      841 gctcgaccgg ggatatccag tttcccggtg cctggcggcc ggctggacgc gttcgccccc
      901 ggggcctctc tcgtgcgttc tgtaaccaaa gcaccgctgg gaggggaagg ctaacgagcg
      961 ccggttccca gggcatcagc ctggcccgga tagactcggg caaccgccac tatacttgtg
     1021 gttggcccca gatgtccgaa actcctgctt gcgccgcaac cagagtggcc cccgacgcca
     1081 ggcggcgccg cccaggtgac ttaccggcga cggcttcgcg gctgtggtac cgtgggcagg
     1141 actttgggtg ttgccgggga aacgagagga agatgcccca cgcggcgggc ggacatctta
     1201 ctggggaaga gcgttatcag aaaggagcca ggactgaaat cgccacgacg gatggccttc
     1261 gggcggtatg actacgctgc tttcacggcc tttttctctt atgccgccgg ctcggtggtg
     1321 gtacccgtgg cgctggtatc gctggcgcgc gacctggggt tttcgctcga gcacggaggc
     1381 atgtcggcag gcggcgnnnn nnnnnntgca tttggggcgc accttctcca tggtggcggc
     1441 gatgctcctc tgcggctttg ccgccgggcg ctggggcaag cgctggactc tcgggacgtc
     1501 cgcggtgctg atgggcgccg gcgtgctttt ctgcgccctg gctcccggat acggcgtgct
     1561 cctcttcgcg ctcacgctgg ccggcctagg agaaggcgtg atcgagggcc tggcaacccc
     1621 attcgtacag gacctgcata ccgatgagcc ggggcgctac atcaactttg cgcattcgtt
     1681 ctggtctatc ggcgtcctgg tcaccgtgct cctgtcgggg gcgctgataa cctatggcgt
     1741 ctcctggcgc gtggtgatcg gcgccgtagc actcttggga ttcttcacgg cgctcctgct
     1801 gctcctaccc gcgcgcaaag gccacgccta tcccgagcgc cccgagccgc ttcactggca
     1861 gacggtatgg gcacaggcgc tcgccatctt ccgcgctcca accttctggc tcttcttcgc
     1921 cgccatgttc gtcgccggag gcggcgagtt ctgcctcacc ttttggtgcg ccagctacat
     1981 ccagctcagc ttcatggatg cggcgtgggc gggaggtgtt ggcacggctt gcttctccgc
     2041 ggggatgttc ctcagccggg ccggatgggg ctatctggtc cggcaagacc agttgaagca
     2101 cctcatcatc tggtcggccc taggcggcac gttggtcacg ctcctcttcc cgcacctcac
     2161 caacctgtgg gccttcttcg cgctcctcgt cgccgccggg gtcgcgcccc gccgcgttct
     2221 cctcatcggc ggggcggccg cgaacccggc ggtggcggcc gtggccgcga cggtcttcgc
     2281 cgtgccgatc cggatccccg cacccggcga gtacgtggcc gacggggccg cgcgccaggc
     2341 cgcctgggcg ctcgccgggg acctgccgga ctggtcga
//
LOCUS       DAOSCV010000718         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29246_length_2377_cov_3.516796, whole genome shotgun sequence.
ACCESSION   DAOSCV010000718 DAOSCV010000000
VERSION     DAOSCV010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29246_length_2377_cov_3.516796"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLY56_15380"
     CDS             <1..555
                     /locus_tag="PLY56_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22909.1"
                     /translation="GKRPLWQVPQAFDWGNYQKDEADKYRAPTLSEERVMTYLCLING
                     AHGLIYYSYSDLLRDPLGFERRWADMLLVGKEVKQLEPALISARPAPKVEVQIDGPAR
                     DAVEWMARADDAGATYILLANPDPEKPATARVGVPRGAQVLLLDHHGTRTLPEAKDAR
                     MRTVSLEPMGAVTVILKPGRAQGR"
     gene            complement(670..1776)
                     /gene="dnaJ"
                     /locus_tag="PLY56_15385"
     CDS             complement(670..1776)
                     /gene="dnaJ"
                     /locus_tag="PLY56_15385"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOJ22910.1"
                     /translation="MAAKRDYYEVLGVDRSASLEEIKRAYRKLARETHPDHNPDDPQA
                     EERFKEINAAYQVLSDAQKREQYDMFGHDGPRMASGGFGEAFGGFGDIFDVFFGGGQR
                     RSTGPVRGSDLEYRLEITLEEAAFGTEKKITVDRVEACDACGGMGVASAEGMQMCPVC
                     RGAGQIRQVQNTLLGQISTVTTCYRCHGQGQIITDPCRSCQGQGRIQRTRDLTVRIPP
                     GVETGTTLRLGGQGEAGMRGGPAGDLYVVLKVREHAVFKRDGLDILCEVPISFTQAAL
                     GAEIQVPTLYGPEKLYVPEGTQTGAAFRIRDKGMPEPNGRRMGDQVIQVRVCTPTKLT
                     EKQKDLLRQFAAAGGEKHSEDKSFFEKIKEFLGG"
     gene            complement(1780..1995)
                     /gene="rpoZ"
                     /locus_tag="PLY56_15390"
     CDS             complement(1780..1995)
                     /gene="rpoZ"
                     /locus_tag="PLY56_15390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOJ22911.1"
                     /translation="MSIRPSFRAETEGKRRNTYELVVLAAKRARQLREGAMCLVDPQP
                     ANPLTAALAEIAEGKIDSEYVQEISEG"
BASE COUNT          426 a          795 c          731 g          425 t
ORIGIN      
        1 gggaagcgcc ctctatggca ggtgccgcag gcgttcgact gggggaacta ccagaaagac
       61 gaagccgata agtaccgcgc tcccacgctc tccgaagagc gggtgatgac ctacctctgc
      121 ctcatcaacg gagcgcacgg cttgatctac tatagctaca gcgacctctt gcgcgaccca
      181 ctcggctttg agcgccgctg ggccgacatg ctcctggtcg gcaaggaggt gaagcagctg
      241 gagccggcgc tcatctcagc gcgtcccgca cccaaggtag aggtccagat cgatggcccg
      301 gcgcgtgacg cggttgagtg gatggcgcgg gccgacgacg caggggcaac ctacatcctt
      361 ctcgccaacc cggatccgga gaagccggcc accgcgcggg tcggcgtgcc ccgtggcgcc
      421 caggtgctgc tcctggacca tcacggcacg cgcaccctcc ccgaggcgaa agatgcgcgc
      481 atgcgcaccg tctcgctgga gccgatgggc gcagtgacgg taatcctcaa gccagggaga
      541 gcccagggcc gctgaggctc cggcaagcca gaaactacgc gagggcgcgg aggagaggac
      601 tcctccacgc cctcgcttgt tgaaacgaag catccgggcg ctcgatggcc gccccttgat
      661 gatggggagc taaccgccca ggaattcctt aatcttctcg aagaagctct tatcctcgct
      721 gtgcttctcg ccgcccgcgg cggcaaactg gcgcaggagg tctttctgct tctccgtcag
      781 cttggtcggc gtgcaaacgc ggacctggat cacctggtcg cccatgcggc gaccgttggg
      841 ctcgggcatc cccttatcgc gaatgcggaa cgccgccccc gtctgggtac cctcggggac
      901 gtacagcttc tcagggccat agagcgtcgg tacctggatc tcagccccga gcgccgcctg
      961 cgtaaaggag atcggcactt cgcagaggat atcgaggcca tcgcgcttga agaccgcgtg
     1021 ctcccgcacc ttgagcacaa catagaggtc cccagcgggc ccgccccgca tgcccgcctc
     1081 gccctggcct cccaggcgta gcgtcgtccc cgtctccacg cccggcggga ttcgtactgt
     1141 caggtcgcgc gtgcgttgaa tgcgcccctg cccctggcag ctacggcaag gatcggtgat
     1201 gatctgaccc tggccgtggc agcgatagca ggtggtcact gtggagatct ggccgaggag
     1261 ggtgttttgc acctggcgga tctggccggc accgcggcag acggggcaca tctgcatccc
     1321 ctctgctgaa gcgacgccca tgcccccgca cgcatcgcac gcctcgacgc ggtcgacggt
     1381 gatcttcttc tctgtgccaa acgcggcctc ttcgagcgtg atctccaggc ggtattccag
     1441 gtcggaaccc cgcacgggcc cggtcgaacg ccgctggcca ccgccaaaga agacgtcgaa
     1501 gatgtcgcca aacccgccga acgcctcgcc gaagcctccc gaagccatgc gcggcccatc
     1561 gtggccgaac atgtcgtact gctcgcgctt ctgtgcgtca cttagcacct ggtaggcggc
     1621 attgatctcc ttgaagcgct cctccgcctg aggatcatcc gggttatgat ccgggtgcgt
     1681 ctcccgcgcg agcttgcggt aggcccgctt gatctcttcg agggaggccg agcggtcaac
     1741 tcccagaacc tcgtagtaat cgcgcttggc agccacgcac taaccttccg atatctcctg
     1801 gacgtactcg gagtcgatct tgccttcggc gatctcagcc agcgccgcgg ttagcggatt
     1861 ggcgggttgc gggtccacca gacacatggc cccttcccgc agttgccggg ccctcttggc
     1921 cgccaacaca acgagttcat aggtgttcct acgcttcccc tcggtctctg cgcggaacga
     1981 tggtcgtatg ctcactccct caccccctac aactgcttga tatacaaata atcattggcc
     2041 gtcattcata gaccacccaa tattatagcg cattcaacga ggagtttgca atgctgcgta
     2101 aactcgtttg ggattccgag ctaaggccga tctgggcttc cctccaaccc cagggaaagg
     2161 gagcgaccgc gagatgatcc tggggtaggc ctatcgcgcg gcgatagatc accgcgctgg
     2221 actgggctac attcacggcg caagcagatc ggcgggggct tgggaggtgg aggcccgaag
     2281 gggctttgcc aggaagcagc gcggtgcttc gagctccgcg cgcaggtgta cagcaacccc
     2341 gggaagcagc gcagcgataa atcaccgcgc tgcttcc
//
LOCUS       DAOSCV010000719         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29296_length_2374_cov_2.425614, whole genome shotgun sequence.
ACCESSION   DAOSCV010000719 DAOSCV010000000
VERSION     DAOSCV010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29296_length_2374_cov_2.425614"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..510
                     /locus_tag="PLY56_15395"
     CDS             124..510
                     /locus_tag="PLY56_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ22912.1"
                     /translation="MSETKTLKSLDDLRRLREEAHRDIRVREATGTKIIIGMGTCGIA
                     AGAREVMRAVLEELDKENIEAHVTTVGCIGMCALEPLLDIVQAGGARITYGKVTPERV
                     PRIISEHILRGQPVEEWVVGRTTSFG"
     gene            521..>1469
                     /locus_tag="PLY56_15400"
     CDS             521..>1469
                     /locus_tag="PLY56_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HOJ22913.1"
                     /translation="MSEPLYRAHVAICAGTWCKTLDPEDIAGEMLAEISRRGLGDEIR
                     VVRTGCRGFCSFGPVAVIYPDGIFYCHVGVKDVPEIVEETLIKGRLVERLLYQEPETQ
                     RRLPRYEEIPFYGKQVRIALRNCGVINPTVIEEYVARGGYEALGRALTEMTPEEVIDA
                     IKRSGLRGRGGAGFPTGLKWEFTRKAPGQPKYIICNADEGDPGAFMDRSILEGDPHSV
                     VEGMTIGAYAIGAREGYLYCRAEYPLAVERVKHAIAQAKEYGLLGENILGTGFSFDLR
                     IKEGAGAFVCGEETALIASIEGRRGEPRPRPPFPAVSGLW"
     assembly_gap    1470..1479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1480..>2374
                     /locus_tag="PLY56_15405"
     CDS             <1480..>2374
                     /locus_tag="PLY56_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOJ22914.1"
                     /translation="AQKGLWGKPTNINNVETWANIPPILTQGPEWFASIGTEKSKGTK
                     VFALSGRVNNVGLVEVPMGISLGDIVYDIGGGIPRGRKLKAVQTGGPLGGCIPASLLN
                     TPIDYDSLTAAGATMGSGGMIVVDEDTCMVELAKFFLTFAAAESCGQCTPCRVGGKQL
                     LDVLTRITEGQGTEADLDRIEELSHVMAESSLCALGKLTPSPVRSTVTHFRDEYLAHI
                     REKRCPAGKCTALVHSRCVNSCPAGVDVPAYLMLVSEGKYAEALAVHRERNPFAAICG
                     RVCPAFCEQKCRRGEIDAPVAV"
BASE COUNT          424 a          782 c          814 g          344 t
ORIGIN      
        1 ccacgcgctc ggagaggccc ggcccacatc gccgcgggcg ccggccgccg agagctccgg
       61 aacaccgaca gttgaacggg agcacgagcc accgcgggcc gctctccccc aggaggagaa
      121 ttgatgagtg agacaaagac gctgaagtcg ctcgatgacc tgaggcgcct gcgggaggag
      181 gcgcatcggg acatccgggt gcgggaagcc actggcacca agatcatcat cggcatgggc
      241 acctgcggga tcgccgccgg cgcgcgcgag gtgatgcgcg ccgtgctgga ggagctcgac
      301 aaggagaata tcgaggcgca cgtcaccacc gtcgggtgca tcgggatgtg cgcgctggag
      361 ccactcctgg atatcgtcca ggccggcggc gcgaggatca cctacgggaa ggttacgccg
      421 gagagagtgc cgcgcattat ctccgagcac atcctccgcg gccagcccgt cgaggaatgg
      481 gtggtaggga ggaccacgag cttcggatag gaggacgcaa atgagcgaac cgctgtaccg
      541 agcccatgtc gccatctgcg ccggcacctg gtgcaagacg ctcgatccgg aagatatcgc
      601 gggagagatg cttgcggaga tcagccggcg cggcctgggc gatgagatac gggtggtgcg
      661 cacgggctgc cgcggcttct gctcgttcgg cccggtggcc gtcatctacc cggatggcat
      721 cttctactgc catgtcggcg tcaaagacgt gccggagatc gtggaggaga cgctgatcaa
      781 gggccgcctg gtcgagcgcc tgctctacca ggagccggag acacagcgcc ggctgccacg
      841 ctacgaggag atcccgttct acggcaagca ggtgcgcatc gcgttgcgca attgcggcgt
      901 gatcaacccc accgtcattg aggaatacgt cgcccgcggg ggctacgaag ccctgggccg
      961 ggcactcacc gagatgacac ccgaagaggt gatcgacgcc atcaagcgct ccggcctgcg
     1021 cgggaggggc ggcgccggat tccccaccgg gctcaagtgg gagttcacgc gcaaggcccc
     1081 cggccagccg aaatacatca tctgcaacgc cgacgagggc gacccgggcg cgttcatgga
     1141 ccgcagcatc ctggagggcg acccgcacag cgtcgtggag gggatgacga ttggcgcgta
     1201 cgccatcggc gctcgcgaag gctacctcta ctgccgcgcc gagtaccccc tcgcggtgga
     1261 gcgggtgaag cacgcgatcg cccaggcgaa ggagtatggt ctcctcggcg aaaacatcct
     1321 gggcaccggc ttctccttcg atcttcgcat caaagagggc gcgggcgcgt tcgtctgcgg
     1381 cgaggagacg gcgctcatcg cgtcgattga aggccggcgc ggcgagcccc ggccccgccc
     1441 ccccttcccc gccgtctccg gcctctgggn nnnnnnnnng cgcagaaggg gttgtggggc
     1501 aagccgacga acatcaacaa cgtcgagacg tgggccaaca tcccgccgat cctcacccag
     1561 ggccccgagt ggttcgcatc gatcggcacg gagaagagca aaggaacgaa ggtgtttgcc
     1621 ctctccggcc gggtgaacaa cgtcggcctg gtcgaggtgc cgatgggaat cagcctgggg
     1681 gatatcgtct atgacatcgg cggcgggatc ccgcgcggac gcaagctgaa agccgtgcag
     1741 acgggaggcc cgctcggtgg gtgcatcccc gcgtcgctcc tcaacacgcc aatcgactac
     1801 gactcgctca ccgcggcggg cgccaccatg ggatccggcg gcatgatcgt cgtcgatgaa
     1861 gacacctgca tggtggagct ggcgaagttc ttcctcacct tcgccgccgc cgagtcgtgc
     1921 gggcagtgta ccccctgccg cgtcggcggc aagcagctcc tcgacgtgct cacgcggatc
     1981 accgagggcc agggcaccga ggccgacctc gacaggatcg aggaactctc gcacgtgatg
     2041 gccgagagct ctctctgcgc gctcggcaag ctgacgccca gcccggtccg gagcacggta
     2101 acccacttcc gcgacgagta cctggcgcat atccgcgaga aacgctgccc cgctggcaag
     2161 tgcacggcgc tcgtccactc gcggtgcgtc aactcctgcc cggctggcgt ggatgtgccc
     2221 gcctacctga tgctcgtttc cgaagggaag tatgcggagg ccctggcggt tcaccgcgag
     2281 cgcaacccgt tcgccgcgat ctgcgggcgc gtctgccccg ccttctgcga gcagaagtgc
     2341 cggcgcggcg aaatcgacgc ccctgtcgcc gtgc
//
LOCUS       DAOSCV010000720         2371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29337_length_2371_cov_3.521157, whole genome shotgun sequence.
ACCESSION   DAOSCV010000720 DAOSCV010000000
VERSION     DAOSCV010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29337_length_2371_cov_3.521157"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1244
                     /locus_tag="PLY56_15410"
     CDS             90..1244
                     /locus_tag="PLY56_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOJ22915.1"
                     /translation="MGQPRPEQAGTDQSQRPTFCILGAGHGGTAMAAHLGLMGFPVRL
                     FNRSPERITPIQLAGSIELVAPDLVDLPQGYAPVQVATTDPEEAIVGADILMVCTPAT
                     GHRYMAQRCAPLLTDGQIIVLNPGRTGGALEFTHVLREDGVRADVIVAEAQTFIYAAR
                     MLNPAQVRIFRVKNSIPVAALPAYRTPEVVRRLRVAFPQFVPGDNVMKTSMDNIGAIF
                     HPAVTVLNSARIESTHGDFEYYLEGITPSVARVLERMDAERVAVGEAMGFQCLTARQW
                     LYMAYDAAGRTLYDAINANPGYAGIRAPRTLDTRYLSEDVPMSLVPISSLGELLGVPT
                     PTIDHVIGLACVLHGVDYRAEGRTAERLGLAGMSVKEIRHYVLEGASLHG"
     gene            1237..2139
                     /locus_tag="PLY56_15415"
     CDS             1237..2139
                     /locus_tag="PLY56_15415"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOJ22916.1"
                     /translation="MDRRAKVLAGALGNCVHVAGVSGFLALAEEAGYDALFLGPAIPP
                     ARFAEAVAEHDPEIVGISYRLTPEVLPGLLTELHAELEARGLMQGRRFVFGGTPPTAS
                     VALETGWIERAFTGFETTEEVIAFLKGEQAGEAEEQYASTQVERLRAKAPYPLLRHHF
                     GLPSVEETVEGVRQIALSKMVDVISLAPDQNAQESFFRPEEMDPRSMGPGASPCARGK
                     TSSGSTRRAGAATTRCCGSTAARATWCAGQSWHTRRSRMPGAPSPSAGTASSTGGRSG
                     ARPKRLPRTERRCAGTRSEGFRLR"
     gene            2100..>2371
                     /locus_tag="PLY56_15420"
     CDS             2100..>2371
                     /locus_tag="PLY56_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOJ22917.1"
                     /translation="MRWYAERGLPVEVNEAHHWSLREAHDAVAVAMAYLAAYNARSVG
                     VRHYIAQYMFNTPAGTYAAMDLAKMLAKIELIESLHTEQFTTYRQVR"
BASE COUNT          400 a          779 c          823 g          369 t
ORIGIN      
        1 agcatcgtgc catggagcaa gctgggctca gaggaggcgt tcctcaactc ctggatcgac
       61 cagggaccac ctcggaaggc gcgagcggca tgggccagcc acggccagag caggcaggga
      121 cagatcagag ccagcgtcca accttctgta tcctgggcgc ggggcatggg ggcacagcga
      181 tggccgctca cctggggctg atgggtttcc cggtgcggct cttcaaccgg agcccggagc
      241 ggatcacgcc gatccagctc gccgggagca tcgagctggt cgccccggat ctggtcgacc
      301 tgccccaggg gtatgcgccg gtgcaggtgg ccaccaccga cccggaagag gcaatcgtcg
      361 gagccgatat cttgatggtc tgcacgccgg cgaccgggca tcgctatatg gcgcagcgct
      421 gcgcgcccct actcaccgac ggccagatca tcgtgctcaa cccagggcgc accggcggcg
      481 cgctggagtt cactcatgtt cttcgcgagg atggcgtgcg cgccgatgtg atcgtcgccg
      541 aggcgcagac cttcatctat gccgcgcgca tgctcaaccc ggctcaggtg cgcatcttcc
      601 gggtgaagaa cagcatcccc gtcgcggcgc tgccggccta tcgcacgccg gaagtggtgc
      661 gccggctccg cgtcgccttc ccgcagttcg tgcccggcga taacgtgatg aagacgagca
      721 tggacaacat cggcgcgatc ttccatcccg ccgtgaccgt actcaactcg gcgcgcatcg
      781 aatcgaccca cggcgatttt gagtactacc tggaggggat caccccctct gtagcgcgcg
      841 tcctggagcg aatggacgcc gagcgcgtcg cggtcggcga ggcgatgggc tttcagtgcc
      901 tcaccgcgcg gcagtggctc tacatggcct atgatgccgc cgggcgaacc ctctacgacg
      961 cgatcaatgc caacccaggc tatgccggga tccgggcgcc tcgcaccctc gacacgcgct
     1021 acctctccga ggacgtgccg atgagcctgg tgccgatctc ctcgctgggc gagcttctgg
     1081 gagtgcccac gcccaccatt gaccatgtga tcggcctggc gtgcgtcctc cacggggttg
     1141 actaccgcgc ggaggggcgc accgccgagc ggcttggcct ggcggggatg agcgtgaaag
     1201 agatacgcca ctacgtcctg gaaggagcga gcctccatgg ataggcgcgc gaaggtcctg
     1261 gcgggggctc tgggcaactg cgtgcacgtc gccggcgtct ccgggttcct cgccctggcg
     1321 gaagaggcgg gctacgacgc gctcttcctt gggccggcca tcccgcccgc gcgtttcgcg
     1381 gaggcggtgg cggagcatga cccggagatc gtcgggatca gctaccggct gacgccggag
     1441 gtgcttcccg gcttgctcac cgagctccat gccgagctgg aggcgcgtgg tttgatgcaa
     1501 ggccggcgct tcgtcttcgg gggcacgccg ccaaccgcgt ccgtggccct tgaaaccggg
     1561 tggatcgagc gcgctttcac cggcttcgag acgaccgaag aggtgatcgc cttcctgaaa
     1621 ggcgagcaag ccggcgaggc agaggagcag tacgcgtcca ctcaggtgga gcgcctgcgg
     1681 gcgaaggcgc cctatccgct tctgcgccac cactttggtc ttccgagcgt ggaagaaacg
     1741 gtggaggggg tgcgccagat cgcgctctcc aagatggtgg acgtgatctc gctggcgccc
     1801 gaccagaacg cgcaggagag cttcttccgc cccgaggaga tggacccgcg ctcgatgggg
     1861 ccgggggcgt cgccgtgcgc acgcgggaag acctcgagcg gatctacgcg gcgagccggt
     1921 gcggcaacta cccgctgctg cggatctaca gcggcacgcg cgacctggtg cgctgggcag
     1981 agctggcata cgagacgatc cagaatgcct ggggcgccat ccccctctgc tggtacagcg
     2041 agctcgacgg gcggtcgaag cggagcacgg ccgaagcgat tgccgagaac cgagaggcga
     2101 tgcgctggta cgcggagcga gggcttccgg ttgaggtgaa cgaggcgcac cactggagcc
     2161 tgcgcgaggc gcacgacgcg gtcgccgtcg cgatggccta cctggcggcc tacaacgcgc
     2221 gcagcgtcgg cgtgcgccac tacatcgccc agtacatgtt caacacgccg gcgggaacct
     2281 acgcggcgat ggatctggcg aagatgctcg ccaagataga gctgattgag agcctccaca
     2341 ccgagcaatt caccacctac cggcaggtgc g
//
LOCUS       DAOSCV010000721         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29375_length_2369_cov_3.065255, whole genome shotgun sequence.
ACCESSION   DAOSCV010000721 DAOSCV010000000
VERSION     DAOSCV010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29375_length_2369_cov_3.065255"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLY56_15425"
     CDS             <1..790
                     /locus_tag="PLY56_15425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOJ22918.1"
                     /translation="FDWALDLHGLARSALVMWASRAPFRAGLAGTREGSRFSYNHIVS
                     VPDAGVHRVDYYASFLEALGIPVPHREMLFYTLPEHAEEAQALLSELAPGHGPLVVVS
                     LTAGRMQKCWMPERFGAVADRLIERHGCRVAVIGSKTDRPYVEQMKAAMSHAPADLCG
                     RIGLKTLGEVLRAANLFLSADTGPMHIAAAMKTPVVALFGPTEPELYGPWRTDSTVIR
                     KHCRCAPRWRHPVCEDRECMRAITVDEVEEAVVALLVRTGALVG"
     gene            1165..1311
                     /locus_tag="PLY56_15430"
     CDS             1165..1311
                     /locus_tag="PLY56_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22919.1"
                     /translation="MLFVAVTVLGFATNGAIVLAAYILLPLDAYFVAARLNRGEAVSQ
                     WQWF"
     gene            1604..>2369
                     /locus_tag="PLY56_15435"
     CDS             1604..>2369
                     /locus_tag="PLY56_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ22920.1"
                     /translation="MKKATLARGLPAGLRATVEPLKRLLPYLGKHRGVAAAALICSVL
                     SQGATLIGPKILQQIVAVVLEAGQRDQLPLLAGAIVAVAAIKGALSFAEIVLGARAGL
                     SVVTSMRADLYAHLTRLSFGYFDRVRTGQLLSRITSDLEPVGGFCTWGFRMIFRSVIL
                     FAGVLAVCIWMNPRLALLSLSTMPLLTATAYFVGERIRPAFERAREQLGVVTTRLREN
                     LSGIRIVKLYVREPLERERYRAASEVLRDRNYEAQRI"
BASE COUNT          346 a          779 c          790 g          454 t
ORIGIN      
        1 cttcgattgg gcgctcgacc tgcacggcct ggcgcgcagc gcgcttgtca tgtgggcctc
       61 gcgcgcaccg ttccgcgccg ggctggccgg cacgcgcgag ggaagccggt tctcctataa
      121 tcacatcgtg agcgtgccgg atgccggcgt acatcgcgtg gactactatg cgtcgttcct
      181 cgaggcgctt gggatccccg tgccccatcg tgagatgctg ttctacacct tgcccgagca
      241 cgcggaggaa gcccaggcgt tgctgagcga gctcgcgccc ggccacggcc cgctcgtggt
      301 cgtctcgctc actgccggca ggatgcagaa gtgctggatg ccggagcgat ttggcgcggt
      361 ggccgaccgg ctgatcgagc gccacggctg tcgcgtcgcc gtgatcggga gcaagaccga
      421 ccggccgtac gtggagcaga tgaaagcagc gatgtcgcac gctcctgccg acctgtgcgg
      481 gcgcatcggc ctgaaaaccc tgggcgaagt gcttcgggcg gccaatctct tcctcagcgc
      541 cgacacgggc ccgatgcaca tcgctgccgc gatgaagacg ccggtggtgg cgctttttgg
      601 tcccaccgag ccggagcttt acggcccgtg gcggacggat tccaccgtga tccgcaagca
      661 ctgccggtgc gcaccgcgct ggcgccatcc cgtatgcgag gaccgcgagt gcatgcgcgc
      721 cattaccgtt gacgaggtcg aagaagccgt cgttgcactg ctcgttcgca ccggcgcgct
      781 ggtggggtag ccctcggaca gctcgctacc cgggttagtc tcgaagggct cccgcgctgt
      841 ctttcgtgcg gggggcattc agtcggcctg gaccagctcg ctcagctggt tccatctgaa
      901 agacgggtgc cgccggcggg tagctttggt gggtcaacgg gtaaccccag cggacctgca
      961 tcaggcgggt tcggcgggcc agtgagtggc ttcggaggat cgcccccgca gccgtcctac
     1021 caccgccctg catcgcagcg atcgcacgga gacctaatcc aggtacgccc tggaacccac
     1081 tcgcccgtca aggcgctgct cctcagtctc ttgtgttgct gtatcttggg acaggtctac
     1141 aacaagcaac ttctaaaggc gattgtgcta ttcgttgccg ttacggtact tgggttcgcg
     1201 accaacggcg cgatagtgct cgcggcctat attctgttgc cgctcgatgc ctacttcgtg
     1261 gcggcacgcc tcaatcgggg cgaagctgtt agccagtggc agtggttctg acaagcgcga
     1321 gagagtgcag gagtcgggtg cgcagcggtc gcgagctgtg catgcgtgga taggacctac
     1381 gagtgctgcc cccgctcggg tttggtggat agcactagcc gggctctcgc acccctgccg
     1441 tcgtcggttt cctctcgtga tccgttttgt ggtataatgc tctcgcgcca ggcatgaatt
     1501 ctcttgggat gcgacggcgt tgcgctcgca tggcgcgatg caagggggcg agtaccgcac
     1561 cctaacactc tgattacggg attggacacg atcactggac ggcgtgaaga aagcgactct
     1621 tgcccgcggc ctgccggccg ggcttcgtgc gaccgttgag cccctgaagc gcctgctccc
     1681 ctacctgggg aagcaccgcg gagtggccgc ggcggcgctc atctgctccg tgctcagcca
     1741 gggggcgact ctcatcggac cgaagatcct ccagcagatc gtggccgtgg tcctcgaagc
     1801 cgggcagcgc gaccagctcc ccttgctcgc gggcgccatc gtggctgtgg ccgctatcaa
     1861 aggcgcgctc tccttcgccg agatcgttct tggcgcgcgc gccggcctct cggtggtcac
     1921 ctctatgcgc gccgacctct atgcccacct cacgcgcctc tccttcggct acttcgaccg
     1981 ggtgcgcacc ggccagctcc tctcgcggat caccagcgac ctggagccgg tcggcgggtt
     2041 ctgcacctgg ggtttccgga tgatcttccg gagcgtcatc ctcttcgccg gcgtgctggc
     2101 cgtctgcata tggatgaacc cgcggctggc gcttctctcg ctgtcgacga tgccgctgct
     2161 gacggcgacg gcctacttcg tgggcgagcg gatccggccc gctttcgagc gcgcgcgcga
     2221 gcagctgggc gtggtgacga cgcggctccg ggagaacctc tccggcatcc gcattgtcaa
     2281 gctctacgtg cgcgagcccc tggagcgcga gcgatacagg gcggcctccg aggtgttgcg
     2341 cgatcgaaac tacgaggcgc agcggatcg
//
LOCUS       DAOSCV010000722         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29418_length_2367_cov_3.945069, whole genome shotgun sequence.
ACCESSION   DAOSCV010000722 DAOSCV010000000
VERSION     DAOSCV010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29418_length_2367_cov_3.945069"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLY56_15440"
     CDS             <1..725
                     /locus_tag="PLY56_15440"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOJ22921.1"
                     /translation="ILDQMATYADQLRPYVDDTISLVTSAVAEGRNVLCEGAQGTMLD
                     IDHGTYPYVTSSHTVAGGACLGTGIGPRHIDRVIGVNKAYTTRVGRGSFPTELHDATG
                     DWIRKQGAEFGTTTGRARRCGWLDMVALRFAVAVNSLDSLALTKLDVLTGLPTVCIGV
                     GYKIDGKVRDSLPHLQEEFDRSEPVYEELPGWTEDITGARRLADLPTNARRYLERVET
                     LAGVPLSMVSVGPRRDQALILD"
     gene            1394..>2367
                     /locus_tag="PLY56_15445"
     CDS             1394..>2367
                     /locus_tag="PLY56_15445"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HOJ22922.1"
                     /translation="MSKSLIIVESPAKTKSLKSFLGSGFQIMASMGHVRDLPKTRLGV
                     RVDHDFQPTYVQIPERKETLENLRKAVQAADRVYLASDPDREGEAIAWHLKEALRLEN
                     PLRIEFNEITPQAVREALQHPRSLDVDRVNAQQARRVMDRLVGYNLSPLLWRKVKRNL
                     SAGRVQSVAVRLIVDREAEIEAFVPVEYWSIAATLTPLDQEFPFQAKLTHHAGKKVEV
                     STGEEAEAIVAALRTATYVVRSIKRRAQRRHPSPPFITSTLQQEAARKLGFSNRRTMS
                     IAQQLYEGIDLGDEGTVGLITYMRTDSVRISQQALDEARAYIADRWGK"
BASE COUNT          434 a          731 c          781 g          421 t
ORIGIN      
        1 cgatcctcga tcagatggcc acctacgcgg atcagctccg gccctacgta gacgacacga
       61 tctcgctcgt cacgagcgcc gtcgcggagg ggcgcaacgt tctctgtgag ggggctcaag
      121 ggacgatgct tgatatcgac catggcacct atccctacgt cacctcctcg cacacggtgg
      181 cgggcggggc ctgcctcgga accggaatcg gcccgcgcca catcgaccgc gtcatcggcg
      241 tcaacaaggc ttacacgact cgggtgggga gggggagctt ccccacagag ctccacgatg
      301 caacgggcga ttggatccgg aagcagggcg ccgagttcgg gaccaccacg ggtcgcgcgc
      361 gtcgttgtgg ctggctcgat atggtcgccc tgcgcttcgc ggtggctgtc aacagcctcg
      421 acagcctggc gctgacgaag ctggatgtgc tcaccggcct ccccaccgtc tgcatcggcg
      481 tgggctacaa gatcgatggc aaggtccgcg atagcctgcc gcatctccag gaggagttcg
      541 acaggagcga gccggtttac gaggagctcc ccgggtggac ggaagatatc accggcgcac
      601 ggcgcctggc cgacctcccg acgaacgcgc gccgatatct ggagcgcgtg gagaccctgg
      661 ccggcgttcc cctcagcatg gtctctgtgg ggccgcgccg cgatcaggca ctgattttgg
      721 actaaggttc acttcaagaa gaggcgtagg ggttttcggc ccctacgccc tcgaatcggc
      781 atgaaaccca ccgccagggc gaagcgcatg cctgctggga agcaggtgcc ggccgagacg
      841 gtggagccac tcaccctccc cttggcgacg cctgatccgc gggctggcgg ttgcgcgatg
      901 gcaacacctt cgcgctgcgc tgcctcatga tccgcatgcc ttctcccgga agtgtggaag
      961 aggatcgcgg gtcgacccgc gtgggcaggc agaagtacgc gctcgcgttg ccgaatgccc
     1021 atgaggctat tgggccgtgg gccgccccct gcggttgtga tggagtgata gctccgtctt
     1081 caaccgggcg attgtgcccg ccgggttggg ggcaactcgc caactggtac gtctgctgca
     1141 gattatctgt tggggatttc ggggaggtat cgacggacgc cgacatcacc gcgaaggggg
     1201 tcaatggagg gcagaagcgc ccggctgtgc gcggttttgg cgtgttgaca ctcagaatcg
     1261 attatgctag aatagccggg ttcgctgccg gaatgccggt ggcgttggga ctacacactc
     1321 tatagcgtgt agtccttttg ccaggtggcc atctctagcc cctgccggca aaggtactgt
     1381 tagaggaaac tttatgtcga aatcgctgat tattgtggag tcgccggcca agacgaagag
     1441 cctgaaaagc ttcctgggtt ccggctttca gatcatggcg tctatggggc atgtccgcga
     1501 tctaccgaag acccgtctgg gcgttcgcgt ggaccacgat ttccagccta cttatgtcca
     1561 gatcccggag cgcaaggaga cactcgagaa cctgcgcaag gctgtacagg ccgcagaccg
     1621 ggtctacctg gcctccgacc ccgaccgcga gggggaagca atcgcctggc atctgaagga
     1681 agccttgcgg ctggagaacc cgcttcgcat tgaattcaac gagatcaccc ctcaggcggt
     1741 gcgcgaggcg ttgcagcacc cgcgctcgct cgatgtggac cgcgtgaacg cgcagcaggc
     1801 gcgccgcgtg atggaccgcc tcgtcggcta caacctgagt ccgcttctgt ggcggaaagt
     1861 gaaacgcaac ctgagcgcgg gccgcgtgca atccgtcgcc gtccggctga tcgtggaccg
     1921 cgaagcggag atcgaggcat tcgtgccggt cgagtactgg tcgattgccg ctacgctcac
     1981 gccgcttgat caggagtttc cgttccaggc gaagttgact caccacgcgg gcaaaaaggt
     2041 ggaggtgagc actggcgaag aggcagaggc gattgtcgct gccctacgca ccgcgaccta
     2101 tgtggtgcgc agcatcaagc ggcgcgcgca acgccgccat ccatcgccgc ccttcatcac
     2161 ctcgacgctg cagcaggagg ccgcgcgcaa gctcggcttc agcaaccggc gcacgatgag
     2221 catcgctcag cagctctacg agggaatcga cctgggcgat gagggcacgg tcggtctcat
     2281 tacttatatg cgcacggact cggtgcgcat ctcgcagcag gcgctcgatg aagcccgggc
     2341 ctacatcgcc gaccggtggg ggaagga
//
LOCUS       DAOSCV010000723         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29419_length_2367_cov_3.714100, whole genome shotgun sequence.
ACCESSION   DAOSCV010000723 DAOSCV010000000
VERSION     DAOSCV010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29419_length_2367_cov_3.714100"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PLY56_15450"
     CDS             <1..326
                     /locus_tag="PLY56_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22923.1"
                     /translation="GHGVEWKVPVARAGRYQLLIRACTAEARALRKLTVNGKTPPGAE
                     AMELRGTGGYSNERDDWRTFVTTGADGRALLLDLPAGEATIRLENIDGESLNLDWIAL
                     APAAE"
     gene            complement(395..1279)
                     /locus_tag="PLY56_15455"
     CDS             complement(395..1279)
                     /locus_tag="PLY56_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOJ22924.1"
                     /translation="MRRTMKYGLLCLAVMAASLSVPGQVSAARAQRVPPMSFLEYRAD
                     TVEALALQAEQNPQVRARYARHFGIPGSRVAQFIRENLVISYLPASGRYRVYYVRPGG
                     RIVARQVYLPRGTRVLALRNGEPVLRWICGNAMVPELPEVAVRPVRQVPQVPEVISVP
                     SEQVVPVSRVVSVPFEQVAVREIVPPVAVAAAPVPVAPVPIPVSRAAAAVPVVAPVPA
                     PTPVPPRPSSVVPILAAVPPITFIGGERPQPPPPITELPPPEIPVPEPGTLAMLTLAA
                     GPMAWAAARGKRHNSRSE"
     gene            complement(1415..2239)
                     /locus_tag="PLY56_15460"
     CDS             complement(1415..2239)
                     /locus_tag="PLY56_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22925.1"
                     /translation="MYPVGGGRESVWQPGMPVYVGDRIVLRMFASTGDARLAEMRVRL
                     DNEPLRTLTQAPWSIEIDTSNLSPGYHFVEVFAVSSDPSADMGSGTTIFYVREPEPVR
                     EAAAPEPRTETTVTTGAVQEMMTEEPTTAVAGVRQTPEEAFQPPAHPGREIPMRDGIP
                     LYQAGLDISLHSADPAVEQMLAQDGVVQVTKPTLFRVTVGNNARRIIYALMRNGQEIY
                     RSDPLPTNALIRLQPKTDADSVGLQPGEVSLWVWAGNEEELYGAPDMARVEIAPQQ"
BASE COUNT          387 a          795 c          809 g          376 t
ORIGIN      
        1 ccgggcacgg ggtggagtgg aaggtgcctg tagcgcgcgc gggccgctac cagctcctca
       61 tccgggcatg cacggcggag gcgcgcgccc tgcgaaagct gacggtgaat ggaaagacac
      121 cccccggagc cgaggcgatg gagctgcggg gcaccggcgg ctacagcaac gagcgcgatg
      181 actggcgtac cttcgtgacg acgggcgccg atggccgcgc gttgctcctc gatctcccgg
      241 ccggcgaggc gacgatccgg ctggaaaaca tcgatgggga gagcctgaac cttgactgga
      301 ttgccctggc gcccgcggcc gagtgaggcg ctgagggcgc actagaggca gatcagggcg
      361 gggaatcccc cgccctgacc cgctgtgccg ctttctactc agaacggctg ttgtggcgct
      421 tgccgcgcgc ggcggcccag gccatcggcc ccgccgctag cgtcagcatc gccagagtgc
      481 cgggctccgg aaccgggatt tccggtggcg gcagctcagt gatcggcggc ggtggctgcg
      541 gccgctcacc accgatgaag gtgatcggcg ggacagcggc caggatcggc accacggagg
      601 aaggccgtgg gggcaccggg gtcggcgccg gcacgggggc taccaccggc acggcggcgg
      661 cggctcgcga taccggaatc ggcacagggg cgaccggcac cggcgcggcg gcaaccgcaa
      721 ccggcgggac gatctccctc accgccacct gctcgaacgg aacgctcacc acccgcgaga
      781 ccgggaccac ctgctcagag ggcacactga tcacctcggg cacctgtggt acttgccgca
      841 ctgggcgcac agcgacctcc ggcagctccg gcaccatcgc gttgccgcag atccaccgga
      901 gcaccggctc tccgttgcgc agcgccagga cgcgggtccc gcgcgggagg tagacctggc
      961 gggcgacgat gcggccgccg gggcgcacgt agtagactcg atagcgtccg ctggccggca
     1021 ggtagctgat caccaggttc tcccggatga actgcgcaac ccgcgaccca gggatgccga
     1081 aatgcctggc gtagcgcgcc cgcacttgag gattttgctc agcctgcagg gccagtgcct
     1141 cgaccgtgtc ggcgcgatac tccaggaacg acatcggcgg cactctttgg gctcgagcgg
     1201 cggagacctg gccaggcacg ctcaggctcg ccgccatcac cgccaggcac agcagtccgt
     1261 atttcatggt tctccgcatg agtcatgctc ctttgttcac gtctcgtgtt gcggaactgc
     1321 accggatata ggcaggcttc cggtccggag ccgagcgaag cgccggcgcc ggcttccgga
     1381 agcgcgcctt aggtgagagg atctgcgaga gccctcactg ctgcggtgca atctccaccc
     1441 gggccatgtc tggtgccccg tagagctcct cctcattccc tgcccagacc cagaggctca
     1501 cctcaccggg ctgcagcccg acagagtccg cgtctgtctt tggctgcagg cggatcagcg
     1561 cgttggtcgg cagcggatcg gagcgataga tctcctgccc attgcgcatc agcgcgtaga
     1621 tgatgcggcg ggcattgttg ccgacggtga cacggaagag cgtcggcttg gtcacttgca
     1681 ccacgccatc ctgggcgagc atctgctcca cggccggatc ggctgaatgg agagagatat
     1741 ccaggcccgc ctggtagagc gggatcccat cgcgcatggg aatctctcgg cccgggtgcg
     1801 caggcggctg gaacgcctct tccggcgtct gacgcacgcc ggcgaccgct gtcgtgggct
     1861 cctccgtcat catctcctgc accgccccgg tcgtcaccgt ggtctcggtg cgtggctcgg
     1921 gagcagccgc ttccctcacg ggctcaggct cgcgcacata gaagatggtt gtcccagatc
     1981 ccatgtctgc cgaaggatcg ctggaaacgg cgaacacctc gacgaagtgg tagccgggac
     2041 tcaggttgct ggtgtcgatc tctatggacc acggcgcctg ggtcagcgtg cgcagtggtt
     2101 cattatcgag gcgcacgcgc atctctgcga gccgcgcgtc cccagtagag gcgaacatcc
     2161 tcagcacgat tcgatcacca acgtagacgg gcatccccgg ctgccagaca ctctcgcgcc
     2221 cgccgcccac cgggtacatc ctcggctcgc cctgaggagt gaaaacctca ggcgtgatcg
     2281 tcacggaagg gatgtaggtt ctctcggttg ttgccgcagg cgctgccggt gccgtaggcg
     2341 cggttgtcca ggaagtactg ttccatc
//
LOCUS       DAOSCV010000724         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29448_length_2365_cov_3.507359, whole genome shotgun sequence.
ACCESSION   DAOSCV010000724 DAOSCV010000000
VERSION     DAOSCV010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29448_length_2365_cov_3.507359"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLY56_15465"
     CDS             complement(<1..510)
                     /locus_tag="PLY56_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ22926.1"
                     /translation="MYVRTVGRHGFTLIELLVVIAIIAILAAILFPVFARARENARKS
                     NCQSNLKQIGLAIRSYSQDYDERYPCYRFINNIDGQTYSWRIAILPYVKNRGIFICPS
                     ARNLNTFAQSTNDRSDYGVAPAASGAFLTSGYYCNSVHYDSGAPNPPFMHRDQGGISP
                     NTPRSARSPW"
     gene            742..>2365
                     /locus_tag="PLY56_15470"
     CDS             742..>2365
                     /locus_tag="PLY56_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22927.1"
                     /translation="MEVRLNMMHGQGCQSVTLRRVLMLLVLGATAMQAEAAAMATLSM
                     NGQTLSAEAGLKFEPGKAHLLRVVPDWARLAEDRITILVRKCLPDGKEEVLRAEAPRG
                     EGRDFELGPLTAPPSYAFERGEVLRRGYRLRVELAAQGTVSPLWSISLVQGQQAAPAR
                     RVPGVPIPAGVERFLPGDEEWSRFLPHRAVGEAIVLRLSEKVLRDPDALIIQAGLAKG
                     ARVQTLPCELRILGPDGLAVYAREVTLAPHAKPTSHPVDPRAWPAGNYIIRLCPIVAG
                     KRWNEGPTLTYRRRAPDPDAVAVSHLAPWTLRRDRTREAWETRDLRKACAEYSGGLPE
                     GSQFEDAEGQSALICPPGMAAEPVELRLPLAGYYALFARATADGCLIQAGEEELVRMV
                     RPGEEVFVCATDLTASVVRVFAFDTFNAPRTGLASLRLVPVTRESVEAFRRETGNPPV
                     PLTGVDDWGEYFHGPVRIAEDQFATIAGGQAELGLRTLAWSVGRSWVEYHSKLPQTTR
                     FPCIPLAEARKLFDRADNYIGRITMQERYDPLE"
BASE COUNT          399 a          712 c          843 g          411 t
ORIGIN      
        1 ccacgggctg cgggccgacc tcggcgtgtt cgggctgatc ccgccctggt cgcggtgcat
       61 gaagggcggg ttcggcgcgc ccgagtcgta gtggaccgag ttgcagtagt agccggaggt
      121 caggaaggct ccggaagccg ccggcgcaac cccataatca gaccggtcgt tcgtgctctg
      181 ggcgaaggtg ttgagattgc gcgcgctggg gcagatgaag atcccgcggt tcttcacgta
      241 gggtaggatg gcgatccgcc agctgtacgt ctgcccatcg atgttgttga tgaaccggta
      301 gcatggatag cgctcatcgt aatcctgcga gtaggagcgg atggccaagc cgatctgctt
      361 cagattgctc tggcagttgg atttgcgcgc gttctcacgt gcccgcgcga acaccgggaa
      421 gaggattgct gccaggatgg cgatgatagc gatcaccact agcagctcga taagagtaaa
      481 accgtgtctg ccaaccgttc ggacgtacat gagacacctc tctctctgac ctgtcctggt
      541 caacagcgcg cccggcaagg tgccgggtca gaggggcgtg aggaagagcc cctgtatgtt
      601 gccgccatta tagcatccga taggaggggt gtcaacatgg aacccgcgag gcgatggcag
      661 gggaccgcgc tggcatcgat accgtggatg aagaggagcc acgatcctgc gagacgaacc
      721 ccagggaaca cggagggccc catggaagta cgcctaaaca tgatgcacgg tcagggatgc
      781 cagagcgtta cactccgcag ggtactgatg ctcttggtcc tgggagcaac cgcgatgcaa
      841 gcagaagcgg cagcgatggc aacgctttcg atgaatgggc agacgctgtc agcggaggcg
      901 ggcctgaagt tcgagccggg aaaggcccat ctcttgcgcg tcgtgccgga ctgggcacgc
      961 ctcgcggagg accggatcac gatcctcgtg cggaagtgtc tcccggatgg aaaagaggag
     1021 gtgctgcgag cggaagcccc tcgcggcgag ggccgcgatt tcgaattggg cccgctcacg
     1081 gcaccgccct cctatgcatt cgagcggggt gaggtgctcc gccgcggcta ccgattgcgg
     1141 gtggagctgg cggcgcaagg gacggtgagc cctctctgga gcatctcgct ggtccagggg
     1201 cagcaagcgg cgcctgcgag gcgtgttccg ggtgtgccca ttcctgccgg cgtcgaacgc
     1261 tttctgccgg gcgatgagga gtggtctcgc ttcctgccac accgcgccgt gggcgaggcg
     1321 atcgttttgc gcctctcgga gaaggtgttg cgcgatccgg atgccctcat cattcaggcc
     1381 gggctcgcca agggcgcccg cgtgcagacg ctgccctgcg agttgcgcat cctcgggccg
     1441 gatggcctcg cggtctatgc ccgcgaggtg actcttgctc cacacgccaa gccaacgagc
     1501 catcctgtag atccgcgcgc ctggccggca ggcaactaca tcattcgcct ctgcccaatc
     1561 gttgccggta agagatggaa tgagggcccg acgctcacct accgccggcg cgccccagat
     1621 cccgatgccg tggccgtcag ccacctggcg ccgtggacgc tccgccgaga ccgcacgcga
     1681 gaggcgtggg agacgcgcga cctgcggaag gcgtgtgcgg agtactcggg cggtctgccc
     1741 gagggttcgc agttcgagga tgcggagggg cagtccgcgc tcatctgtcc tccgggcatg
     1801 gccgcggagc cggtggagct gcgcctgccc ctggccgggt actatgcgct tttcgcccgc
     1861 gcgaccgccg atggctgcct gatccaggcg ggcgaagagg agctggtgcg tatggtgcgc
     1921 cccggcgagg aggtcttcgt ctgcgcgacg gatctgaccg cgagcgtggt gcgcgtgttt
     1981 gctttcgaca cgttcaacgc gccgcgcacc gggctggcga gcctgcgcct ggtgccggtg
     2041 actcgggagt cggtggaggc ctttcggcgc gagacgggga atccaccggt gccgctcacg
     2101 ggcgtcgatg actgggggga gtatttccac ggcccggttc ggatcgccga ggaccagttc
     2161 gcgacgatcg cgggcgggca ggcggagctg ggtctgcgca cgctggcttg gtccgtgggc
     2221 cgctcctggg tggagtatca cagcaaactg ccgcagacga cgcgcttccc gtgcattcca
     2281 ctggccgaag cgagaaagct gttcgaccgg gcggacaact atattggacg catcacgatg
     2341 caggagcgtt acgacccgct ggagt
//
LOCUS       DAOSCV010000725         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29468_length_2363_cov_4.674610, whole genome shotgun sequence.
ACCESSION   DAOSCV010000725 DAOSCV010000000
VERSION     DAOSCV010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29468_length_2363_cov_4.674610"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLY56_15475"
     CDS             <1..678
                     /locus_tag="PLY56_15475"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ22928.1"
                     /translation="AETPAVLLEEMDFEGVSEALVYHTAMRDDHPLWGNELVVEATAG
                     HPRLHPTWAILPPQTEELGTVEEFLAGMKRHGVKALRAFPTPHRYLLNGTTFGPLFEE
                     MRARRIPLIVPGDWPMVEGLLRDFPDLTVIANQLSNHGQDRYFRPLIERYPRFYVDTT
                     RYECDGGIAAFCRKYGPERMLFGSGFPDIPFFGGVLTLMHADIPDGAKEAIAGGNLRR
                     LLEEVAL"
     gene            675..1481
                     /locus_tag="PLY56_15480"
     CDS             675..1481
                     /locus_tag="PLY56_15480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ22929.1"
                     /translation="MTNPSVLAQEFFAHGRAAECPVIDMHTHPGPYSAIYFPNATPER
                     MIETMDRSGVRLMCAVSHAALVETARGNAYTEAIVRKYPDRVRAYWGLNPNYPERVRA
                     EVADFENHAGFVGFKFLADYHRVPITDEAYVPALEYAHERKLLILIHTWGRSAYDSPD
                     HVEPLAKRYPGATLLMAHCGYGEWDKSIRLAVEYPNVYLEITGTHNPRGIIEKMVRGA
                     GSEKVLYGTDLPWFDPHYVIGCVLCARITDEDRHNILHRNAERLLQPFGV"
     gene            1491..2132
                     /locus_tag="PLY56_15485"
     CDS             1491..2132
                     /locus_tag="PLY56_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018925708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HOJ22930.1"
                     /translation="MQWLTPEKARIFRATHIDNMPWILDHGLYCQSAAVTDPEFRPIG
                     HPDIILKRTRRIVPIEPRGVLSDYVPFYFTPRSPMLYNILTGFGGLQQIPAEEIVILV
                     TSLHAFAQHDVRFVFTDRHAYLQAARFFNALEDLEQLDWRILRASDFRRDPNDPGKME
                     RYQAEALVYHHLPVTLLQGIACFDAEGVSMLRAEVDRRGLSVPVRACPGWYLR"
     gene            2129..>2363
                     /locus_tag="PLY56_15490"
     CDS             2129..>2363
                     /locus_tag="PLY56_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010955665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOJ22931.1"
                     /translation="MIHYMQGNLLQSRAEALVNTVNEVGVMGKGIALAFREAFPASAR
                     LYREACRRGEVRVGRVLVTKNVALFGPRWIIHFP"
BASE COUNT          427 a          740 c          741 g          455 t
ORIGIN      
        1 gcggagacgc ccgccgtgct cctcgaggag atggatttcg agggggtttc cgaggcgctc
       61 gtctatcata cggcgatgcg cgacgaccat cccctgtggg ggaacgagct ggtggtggag
      121 gccacggccg gccatccccg gcttcatcct acctgggcta tcctgccgcc gcagaccgag
      181 gagctgggca cggtagagga gtttctcgcc ggcatgaagc ggcatggcgt gaaggcattg
      241 cgcgccttcc cgacgcctca ccgttacctg ttgaacggca cgacgttcgg ccccctcttc
      301 gaggagatgc gcgcccggcg catccccctg atcgtgccgg gcgactggcc gatggtagag
      361 gggctgttgc gcgatttccc ggacctgacg gtgatcgcca accagctcag caatcacggc
      421 caggaccgct acttccgccc gctcatcgag cgctacccgc gcttctacgt agacacgacg
      481 cgctacgagt gtgatggcgg catcgcggcg ttctgccgca agtatggtcc ggagcgcatg
      541 ctctttggca gtggctttcc cgacattccc ttcttcgggg gcgtgcttac cctgatgcat
      601 gccgacatcc ccgacggcgc gaaagaggcc attgccgggg gcaacctgcg ccggctgctg
      661 gaggaggtgg cgctatgacg aatccctcgg tgttggctca ggaattcttt gcgcacgggc
      721 gcgccgcgga gtgccccgtg atcgacatgc acacccatcc gggtccatat tcggcgatct
      781 actttcccaa cgccaccccg gagcggatga ttgagacgat ggatcgcagt ggcgtccgcc
      841 tcatgtgcgc cgtgtcgcac gcggcgctcg tcgagacggc gcgcggcaac gcctataccg
      901 aggcaatcgt gcgcaaatac cccgaccggg tgcgcgccta ctggggccta aaccccaact
      961 accccgagcg cgttcgcgcg gaggtggcgg atttcgagaa tcacgctggc tttgtcggct
     1021 tcaagtttct ggcggattac caccgcgtgc caatcacgga cgaggcctac gtgccggctc
     1081 tggagtatgc ccacgagcgg aagctgttga tcctgatcca cacctggggt cgcagcgcct
     1141 acgattcgcc ggaccacgtc gagccgctgg cgaagcggta tccgggtgcc accctcctga
     1201 tggcgcactg tggctacggc gagtgggaca agagcatccg tctggctgtc gagtatccca
     1261 acgtctacct ggagatcacg ggaacgcaca atccgcgcgg catcatcgag aagatggtcc
     1321 ggggcgccgg ctcggagaag gtgctctacg gcacggatct cccgtggttc gacccgcatt
     1381 acgtcatcgg ctgcgtcctg tgtgcccgca tcaccgatga ggaccggcac aacatcctcc
     1441 accggaacgc agagcgcctc ctgcagccgt tcggagtata gctcgccgtg atgcagtggt
     1501 tgacgcccga gaaagcgagg atcttccgtg ccacccacat cgataacatg ccctggatac
     1561 ttgaccacgg cctatattgc cagtcagcgg ccgtcaccga tccagagttc cgaccgatcg
     1621 gccatccgga tatcattctg aagcgcacac gcaggatcgt gcccatagag ccacgcgggg
     1681 tgctgagtga ctatgtcccg ttctacttta cacctcgctc acccatgctg tacaatatcc
     1741 ttactggctt cggaggattg cagcagatac cggcggagga gatcgtcatc ctggtgacat
     1801 cgcttcatgc gttcgcccag catgatgtgc ggttcgtctt cactgaccgg catgcttacc
     1861 tgcaagccgc gcggttcttc aacgctcttg aggatctaga gcaactggat tggcgaatcc
     1921 tccgcgccag cgatttccgc cgcgatccca atgatccggg caagatggag cgataccagg
     1981 ccgaagcgct agtataccat catctgccgg tcacgctgct gcaaggtatc gcctgcttcg
     2041 atgcagaagg ggtaagtatg ctgcgggccg aggttgaccg tagaggtctg agtgtgcccg
     2101 tcagggcatg cccggggtgg tacctccgat gatacactat atgcaaggca atctgctgca
     2161 gtcgcgggca gaggccctcg tgaacacggt caacgaggtg ggggtgatgg gcaaggggat
     2221 cgcgcttgcg ttccgcgaag cctttcctgc gagcgctcgc ctttatagag aggcctgcag
     2281 gcgcggagag gtccgggtgg gaagagtact cgtgaccaag aatgtggcgc tcttcgggcc
     2341 gcgatggatt atccacttcc cca
//
LOCUS       DAOSCV010000726         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29557_length_2359_cov_3.141059, whole genome shotgun sequence.
ACCESSION   DAOSCV010000726 DAOSCV010000000
VERSION     DAOSCV010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29557_length_2359_cov_3.141059"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1524)
                     /locus_tag="PLY56_15495"
     CDS             complement(172..1524)
                     /locus_tag="PLY56_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOJ22932.1"
                     /translation="MSEQQANGRITRREVLRRVLIGGAGLALADRCVLRAAEKPARPA
                     AVRPARAKSVIQIWMWGGPAHTDTFDPKPDAGYDYTGPFNKAIPTNVDGIQVSEWLPM
                     LAQHADKYSIIRSMTHHNNGHETAAYIVQTGHQPGDRIVYPCVGAVVSLFKGYGEGYQ
                     GLIPPYVVLTRPQGRFSESGFLGPRYKPFATGGDPNQPRFVVDGIVAEGVSDERQRSR
                     RELLHALDTLGKVMKGDPRFERLDACEEKAYELILGDGAKLFDLSQEKPELRERYGRN
                     TFGQSCLMARRLVEQGVPYVTINYGGWDTHKQHFETMRRKLPEMDRAFSALLEDLSNR
                     GLLDTTIVWWSGEFGRTPRVQWEAPWNGGRGHHGACFCAVVAGGGFKGGHVVGASDAK
                     GMEVADRPVHPRDLIASIYELLGIDPDGPLPNPRGLDVKVLPAAAGGEPGVGRLREIM
                     "
     gene            complement(1590..>2359)
                     /locus_tag="PLY56_15500"
     CDS             complement(1590..>2359)
                     /locus_tag="PLY56_15500"
                     /inference="COORDINATES: protein motif:HMM:NF019223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HOJ22933.1"
                     /translation="SPQNPWFARAAVNRVWSWLMGRGIIHETDDIRPDNPPVNPALLS
                     YLEKEFVASKYDLRHLYRLIVNSRTYQQSPIPRSKQPEAHAYFAYYPVRRLDAEVLID
                     ALCWIGGTGEGYASPIPEPFTFIPEEHRTITLADGSISSSFLEMFGRPTRDTGLESER
                     NNEPTDAQRLYLLNSSDVQRRIRNSPRLRPAYNSVGKNRAEAIRSIYLTVLSREPTAE
                     EMATADAYIQGKGTAAREGAEDLAWALVNSKEFLYRH"
BASE COUNT          381 a          798 c          763 g          417 t
ORIGIN      
        1 ggccagcgcg cgcgtccaat cactccatat gccttgtctc atccgctacc cccagagccc
       61 gcaggctggt ggaggaacga ttccacgaga gaggccggtt tccctggagg cgcgagagag
      121 gcatagccct gacggccacc ccaacacgag cagagatccc ccggataccc gctacatgat
      181 ctcccggagc cggcccacgc ccggctcccc gcccgcggct gccgggagca ccttcacatc
      241 gagcccgcgc gggttcggca gtggcccatc cggatcgatc cccagcagct cgtagatgct
      301 ggcgatcagg tcacgcgggt gcactggccg gtccgcgacc tccatgcctt tcgcgtccga
      361 ggcgccaaca acgtgcccgc ctttgaaccc gcccccggcc acgacggcgc agaagcaggc
      421 gccgtggtgc ccccgtccac cattccaggg ggcttcccac tgcacgcgcg gcgtccgccc
      481 gaactcgccg ctccaccaca cgatcgtggt atcgagcagg ccccggttgg agaggtcttc
      541 gagcagcgcg gagaaggcgc ggtccatctc ggggagcttc cggcgcatcg tctcgaagtg
      601 ctgcttgtgc gtgtcccacc cgccgtagtt gatggtgaca tagggcacgc cttgctccac
      661 caggcgccgc gccatcaggc acgactggcc aaaggtgttg cgcccatagc gctcgcgcag
      721 ctccggcttc tcctgcgaga ggtcaaacag cttcgcgccg tcgcccagga tcagctcgta
      781 ggccttctcc tcgcaggcat cgaggcgttc gaagcgaggg tcgcccttca tcaccttgcc
      841 gagggtatcg agcgcgtgga gtagctcgcg ccgactacgc tggcgctcgt ccgagacgcc
      901 ttctgccacg atcccatcca ccacgaagcg cggctggttc ggatcaccgc cggtggcgaa
      961 cggtttgtag cgcgggccga ggaagcccga ttccgagaac cggccttgcg gccgggtaag
     1021 gacgacgtag ggcgggatca ggccctgata gccctcgcca taccctttga agagcgagac
     1081 cacggcgccg acgcagggat agacgatccg gtcgccaggt tgatgcccgg tctgcacgat
     1141 gtaggcggct gtctcgtgcc cgttgttgtg gtgagtcatg ctgcggataa tcgaatactt
     1201 atccgcgtgc tgcgccagca tcggcagcca ctcgctcacc tgaatgccgt ccacattggt
     1261 aggaatcgcc ttgttgaagg ggccggtgta gtcatagccg gcgtccggct tcgggtcgaa
     1321 cgtgtcggta tgcgcggggc cgccccacat ccagatctgg atgacgctct tcgcccgggc
     1381 gggtcggacc gcggcggggc gcgccggctt ctcggcagct cggaggacac agcggtcagc
     1441 gagggccagg cccgcgccac cgatgaggac tcgccgcagt acctcgcgcc gggtaatccg
     1501 cccattcgct tgctgctcgc tcatcgcctt ctcctccgcg cagggcaccg gtatcgcgcg
     1561 tggcgattcg ggcaccctcg aatgtgacct caatgccggt agaggaactc cttgctgttg
     1621 accagcgccc aagccagatc ctcggcacct tccctcgccg cggtgccttt tccctggatg
     1681 taggcgtccg ccgttgccat ctcctccgcg gttggctcgc gtgagaggac ggtcagatag
     1741 atcgagcgga tcgcctcggc ccggttcttc cccaccgagt tgtaggccgg gcgcaaccgc
     1801 gggctgttcc ggatccggcg ctggacgtca ctggagttga gcaggtagag ccgctgggca
     1861 tccgtgggct cgttgttccg ctcgctttcg agacctgtgt cgcgcgtcgg acgcccgaac
     1921 atctccaaga acgagctgga aatgctccca tcagccaggg tgatcgtccg gtgctcttcc
     1981 gggatgaaag tgaacggttc cggaatgggg ctggcgtagc cctcgccggt tccgccaatc
     2041 caacagaggg cgtcaatcag cacctccgcg tcgagacggc gcaccgggta gtacgcgaag
     2101 tacgcgtgcg cttcgggctg cttgctgcgc gggatcggcg actgctggta ggtgcgcgag
     2161 ttcacgatca gccggtagag gtggcgcagg tcatacttcg aggccacaaa ctccttctca
     2221 agatagctca ggagcgccgg gttcacgggt gggttgtctg gccgaatatc gtccgtctcg
     2281 tggataattc cccgccccat gagccatgac cagacgcgat tcacggcggc gcgcgcgaac
     2341 cacgggttct gcggggaga
//
LOCUS       DAOSCV010000727         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29569_length_2358_cov_3.948762, whole genome shotgun sequence.
ACCESSION   DAOSCV010000727 DAOSCV010000000
VERSION     DAOSCV010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29569_length_2358_cov_3.948762"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1015..>2358)
                     /locus_tag="PLY56_15505"
     CDS             complement(1015..>2358)
                     /locus_tag="PLY56_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015226222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ22934.1"
                     /translation="IYRSTLGCFSGEGGQVWQHGEWQPASKAEIAQRLADGKLRVLVC
                     TDAASEGLNLQAASALINYDLPWNPSKVEQRIGRIDRIGQRQPVLPVRNLFLADSVDT
                     AVYGALRLRCGLFERFVGHMQPVLALAHKRLREGLRRGELGEFIRQLEQEEQKAREDA
                     AVVYAFPTSEAHPVPKTESPVTREDIEGALAALKEGVGNVKATPVRGQRKWRLRGLGG
                     GSVEVTIDREPLEQDGRVLPLTALDGVALRLCERLPLTGDVPLVLAEHAEGPFRCVEA
                     RWVGVDRTVPVTSARQLADLLEGWTGKPAEEQALQAARDEASRSARERVEQMRRAVLG
                     AEKAGLRRQHEAARLRLLRALARTLRCAGQRDLNDLFRHQISQEDGEDGRYHRALTLL
                     GGFPQWTADQIADADAFAEGLTPTDRRNQVALPSVLDAALRDPRWRAACAVGRLH"
BASE COUNT          374 a          853 c          737 g          394 t
ORIGIN      
        1 ctcccggctg gagaagagga cgcctcccca ggaggagaag gaagttgccc tcctgatacg
       61 aatcgtcgtg cgtaacggcg ccggggaacg gcaggacggg gagatgttcg tgctgcgctg
      121 gacccggcgc gcgtagaggc ccgtagaggc gcgatctgac ccctgtccga gttgagacac
      181 gggcgcgccg ggaaacgccc gttgtaggcc ctcctggcgc gttctgaggc atcctgcggg
      241 cgcccgcagg atgccttgtc acattggtca cacttagaag ccaagtcaac ataatagcac
      301 ttatcggagt atgtaggtac cacgcggctt cccggcaccc ccctgtcgca ccctctccgg
      361 caccttcccc aacgccgtct ctttgccgac tgcttttcct actgccgccc tgtttgccgc
      421 cccaccttcc tccttctcgg ggagaaggag gcccccaacg gacggcgaac ggaggcgaaa
      481 gagagaggcc cggggggtaa gcccgggcct ggtgaacgga gtaggttgcg ccgggaactc
      541 cgtctgacca tcgtttcggc atccctcgcg gagttcctcc tgctcttctt caccaggttt
      601 ccgttccacc ccggcccgtg tgcaggcgcg cgggaagtga ccgcttgatt cgccggttcc
      661 gagacggcgc ggaggtgcca aaaacccgca tggtacctgg attcttcccc gaaaagcggg
      721 ggagttcatg acacgcaagc tatgcgatgt tccagatggg ccgaactctc agcggggagg
      781 cgcgcttgtg gaccacgcag ggcgctgatc ggcgctcagg cgcacggcgg tcttctgctc
      841 agttggcggt gagggcggcc ctccccacct tcgcggcgat gcgctgcacg ttctcggtgc
      901 gcaccaccgg ggcgacgccg ggcgcgtcct gctcaggacg aggtaacacc caggctctcc
      961 gtaggcgccg gatctcgcca gccatgcagc gccgcacgcc ggcgctgaag tggtctagtg
     1021 aaggcgaccg actgcacagg cggctcgcca ccgcggatcc cggagggcgg catccaggac
     1081 gctgggaagg gccacctgat tccgccggtc cgtgggcgtc aggccctccg caaaggcgtc
     1141 agcatcggcg atctggtcgg cagtccactg ggggaacccc cccagtaaag tgagcgccct
     1201 gtggtagcgc ccatcctccc cgtcctcctg actgatctgg tggcggaaga ggtcgttcag
     1261 gtctctctgc ccggcgcacc gcagcgtccg tgccagcgcc cgcagaaggc gcagacgggc
     1321 agcttcgtgc tggcgccgca gccccgcttt ctctgcaccc agcacagccc gccgcatctg
     1381 ctccacccgt tccctggcag accgtgacgc ctcgtctcga gcggcttgca acgcctgctc
     1441 ttctgccggc ttccctgtcc aaccctccag caagtcggcc aactgccgcg cactcgtcac
     1501 tggaacggtg cggtcgactc ctacccaccg ggcctccacg caccggaacg gcccctccgc
     1561 gtgttcagcc agcaccagtg gcacatcgcc cgtcagcggc agccgctcgc acaaccgcag
     1621 tgctaccccg tccagcgcgg tgagcggcag cacacgcccg tcttgctcga gcggctcgcg
     1681 gtcgatggtc acctccacgc tcccgccacc gaggccgcgg agccgccact tgcgctgacc
     1741 gcggaccggc gtggccttca cattgcccac cccctccttc agggcagcga gcgctccctc
     1801 gatgtcttcc cgggtgactg gcgactcggt cttgggaacc gggtgcgcct ccgacgttgg
     1861 gaaggcgtag accacggcag cgtcctcgcg cgccttctgc tcctcctgct ccaactgccg
     1921 gatgaactca ccgagttcgc cccggcgaag tccttcgcgc agacgcttgt gagccagggc
     1981 gaggacaggc tgcatgtggc ccacaaagcg ctcgaagagg ccgcaacgca gccggagcgc
     2041 cccgtagacc gccgtgtcca cactgtctgc cagaaagagg ttccgcacgg gcagcacggg
     2101 ctgccgctgg ccgatccggt cgatgcgtcc gatgcgctgc tccaccttgc tcgggttcca
     2161 cggaagatcg tagttgatca gagcgctggc cgcctgcagg ttcagacctt cactggccgc
     2221 atcggtgcac accagcaccc gcagcttccc gtcagcgaga cgctgggcaa tctctgcctt
     2281 cgaggcgggc tgccactctc cgtgctgcca cacctgcccc ccctcgccgc tgaagcaccc
     2341 cagggtagac ctgtaaat
//
LOCUS       DAOSCV010000728         2358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29571_length_2358_cov_3.383413, whole genome shotgun sequence.
ACCESSION   DAOSCV010000728 DAOSCV010000000
VERSION     DAOSCV010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29571_length_2358_cov_3.383413"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="PLY56_15510"
     CDS             complement(<1..676)
                     /locus_tag="PLY56_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOJ22935.1"
                     /translation="MANAVGVILAAGPSLRMKTDTPKPLHRVCGRPLLEYALDAAVES
                     ELSAAAVVIPAGAEAMVALVGERARVVAAGDTVAASITLALATLPEAEHLLVIPADLP
                     QLRAHHLKSLLDCHIAQAANATLLVAGGVPTGVYALRREVALSLDSPAALDAVIASAA
                     TCNVADEPLIDVDTRADLARAEKALRREVIARLMDEGVTFIDPESTFVDAGVRIGRDT
                     VVYPWTF"
     gene            complement(811..884)
                     /locus_tag="PLY56_15515"
     tRNA            complement(811..884)
                     /locus_tag="PLY56_15515"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:Gln,seq:ttg)
     gene            1113..1790
                     /locus_tag="PLY56_15520"
     CDS             1113..1790
                     /locus_tag="PLY56_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ22936.1"
                     /translation="MGEVLAALSPWGDILGRLLVAALCGGLIGFERESADRPAGFRTH
                     LLVCVGSAVYMLTSTMVAGKTHDPGRIAAQVASGIGFLGAGTILKQGSMVRGLTTAAS
                     LWVVAGIGLAAGFGGGGAGIAFLGTLIVLISLNTLKILERRLDRAHRCDVRLTIARPR
                     DRMEWLKEVLEAHEIKVRGLSITDIGDGFGKITIAGRVATRARLEEAVRQLAQHKSVS
                     GVDWEFQ"
     gene            1851..>2358
                     /locus_tag="PLY56_15525"
     CDS             1851..>2358
                     /locus_tag="PLY56_15525"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOJ22937.1"
                     /translation="MRTNQVKQQLREGKTTLGSWLTLPDVIATHYMARVGFDWLTVDM
                     EHSPTSLETATLMFQAIAQAGCVPLARVPWNTPENVKRVLDSGAFGVIFPMQNTRAEV
                     EQAVEATLYPPQGFRSVGASLPALAFDTDAGTYFQRANEEILIVIQIEHIQAVERADE
                     LLSVPGVDA"
BASE COUNT          431 a          730 c          778 g          419 t
ORIGIN      
        1 tgaaggtcca gggatagacc acggtatctc ggccgatgcg gacgccggca tccacgaagg
       61 tgctctccgg gtcgatgaag gtgacgccct cgtccatcaa tcgggcaatc acttcccggc
      121 gcaacgcctt ctccgcgcgc gccaggtcgg cccgcgtgtc cacatcgatc aggggttcgt
      181 ctgccacatt gcaggtcgcg gcgctcgcaa tgaccgcatc gagcgcggcc ggcgaatcga
      241 gagagagggc gacctcccgg cgcaaggcat agacgcctgt gggcacgccg ccagccacca
      301 ggagagtggc atttgccgcc tgggcgatgt ggcaatcgag caacgacttc aagtggtggg
      361 cccggagttg cgggaggtcg gccggaataa caaggaggtg ttccgcctcc gggagggtgg
      421 cgagggccaa ggtgatgctc gcggctaccg tgtcgccggc ggcaacgacg cgggcccgct
      481 ctcctaccag tgcgaccatc gcctctgcgc ccgcgggaat caccaccgcg gcggccgaaa
      541 gctcgctctc gaccgccgca tccaacgcat actcgagcag tggccggcca cacacgcgat
      601 gcagcggctt gggcgtatcc gtcttcatgc gcagggaagg cccagcagcc aggatcaccc
      661 ctacggcgtt ggccatagtc acctcggctc tcccgaacat gcgaaaagcc cgaaaccgct
      721 cctcccacca ggaatcacag cccccttcgc tttagcgctc ttggggccat tcggacaagc
      781 cgttcgggcc ttcaaacaaa ctgcgatcat tggctccggg gcaaggattc gaacctcgat
      841 aggcagaccc aaattctgct gtcctaccat tagacgaccc cggaccgcat tagagctact
      901 tcattatact cggcaacgcc cccgcgtgtc aaccatgagg gtgcttcgaa tcgccccccc
      961 tgcgcgtctc cttaatggcc agccttcctt caggccctcc tgctccttga cgtgggtggt
     1021 ccagagaagg cattcgcgtc gatgtgtgga acatttgtcc ccaaccgcgt gctgcttgat
     1081 gaagtttacc cccgcgccgt gaggaggaga cgatgggaga ggtgttggct gcgctgtctc
     1141 cctggggaga cattctaggg cgactgcttg tggccgcgtt gtgtggcggg ctcatcgggt
     1201 tcgagcggga gagcgcggac cggcctgcag gttttcggac gcatctcttg gtgtgcgtgg
     1261 gttccgccgt ttacatgctc acttcaacga tggtggccgg gaaaacgcac gatcccgggc
     1321 gcatcgcggc ccaggtagcc agcggcatcg gtttcctggg ggccggcacc atcctgaagc
     1381 aggggagtat ggtccgtggc ctgacgactg cagcgagcct ctgggtggtg gcgggtatcg
     1441 gcctggccgc gggcttcggc gggggaggcg cggggattgc gtttctgggc actctcatcg
     1501 ttctgatctc cttgaatacc ctcaagattc ttgaacgacg cctggatcgg gcccaccgct
     1561 gcgacgtgcg cctcaccatt gcccggccgc gcgacaggat ggaatggctg aaggaggtgc
     1621 tcgaagcgca tgagatcaag gtgcgcggcc tctccatcac cgacatcggc gacggcttcg
     1681 ggaagatcac tatcgccggg cgggtagcga cgcgggcgcg cctggaagaa gcggtacgcc
     1741 agctcgctca acacaagtca gtgtccggcg tcgattggga gtttcaataa gcacgtaggg
     1801 agcaggcgtg gataggagta tctggcgcga aggccggagg aggggcaaag atgcgcacga
     1861 accaggtgaa gcaacagctg cgtgagggaa agaccaccct cggttcctgg ctaaccttac
     1921 cggatgtgat cgccacccac tacatggcgc gggtcggctt cgactggttg actgtggaca
     1981 tggagcacag cccgacgagc ctggagacag cgacgctgat gtttcaggcg attgcccagg
     2041 cggggtgtgt gcccctggcc cgcgtaccgt ggaacacgcc ggagaacgtg aagcgcgtgc
     2101 tggatagtgg cgcgttcggc gtcatcttcc cgatgcagaa cacccgtgcc gaggtagaac
     2161 aggcggtcga ggcgacgctt tacccaccgc aaggctttcg cagcgtcggt gcgtcgctac
     2221 cggcgctcgc tttcgacacc gatgcgggca cctacttcca gcgtgccaat gaggagatcc
     2281 tgatcgtcat ccagatagag cacatccagg ccgtcgagcg cgcggatgag ctattgagcg
     2341 tgccgggcgt ggacgccg
//
LOCUS       DAOSCV010000729         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29610_length_2356_cov_2.931334, whole genome shotgun sequence.
ACCESSION   DAOSCV010000729 DAOSCV010000000
VERSION     DAOSCV010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29610_length_2356_cov_2.931334"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLY56_15530"
     CDS             complement(<1..189)
                     /locus_tag="PLY56_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOJ22938.1"
                     /translation="MRASELIRQAQEAELLRFTTAGSVDDGKSTLIGRLLHDARGIYE
                     DHLLAVREASARSGQKEID"
     gene            complement(347..1267)
                     /gene="cysD"
                     /locus_tag="PLY56_15535"
     CDS             complement(347..1267)
                     /gene="cysD"
                     /locus_tag="PLY56_15535"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOJ22939.1"
                     /translation="MSTIHTEALRLNTLDALEAESIHIIREVVAEFERPVMLYSVGKD
                     SSVMVRLAQKAFAPGPIPFPLLHVDTGLKFPEMYSFRDEFCRSIGAELRVYRYEEAIA
                     QGVNPWDWGTVRCCAALKTQALLNALKEGGYDAAFGGARREEERSRAKERVYSFRDTF
                     GQWDPKNQRPELWNLYNGRIARGESIRAFPLSNWTELDIWLYIHREQIPVVPMYFAAE
                     REVVVREGRLIPVYERTRLLPGEKPEKVLCRCRTLGCYPCTGAIRSSARTVEEIIEEM
                     MVVRQSERITRLIDHDQDSSMEQKKREGYF"
     gene            1463..>2356
                     /locus_tag="PLY56_15540"
     CDS             1463..>2356
                     /locus_tag="PLY56_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOJ22940.1"
                     /translation="MIRRDRALRLPGGEILEEAIVEALVPPIRRLTRAEVFSHSHLAL
                     DLKRIHSHPSTGVHTHEFSELVVVLRGTGIHVTPGGEYPIGAGDAFVLHGDEAHGYMN
                     TEDLDLVNILFSLEELGLPRADISALPGFHVLFTLEPKYRRRDRFESRLRLSPEKLHH
                     VAGLIDGLEKELVRRDPGWAFAATAYFMLIVSDLSRFYSHAEEPAVQPLLRLGRVVSH
                     MERHFADAVSLDDLARVGCLSRRTLTRVFRNAFGCSPIEYLTRLRINHAMALLQDADL
                     SITEIAFQVGFRDSNHFSRRFR"
BASE COUNT          399 a          760 c          730 g          467 t
ORIGIN      
        1 atcgatctct ttctgaccac tgcgcgccga tgcttcgcga accgctagga ggtggtcctc
       61 atagatgcct cgcgcatcat gcaacaggcg gccaatgagc gtggacttac catcatcgac
      121 gctgcccgcc gtggtgaagc ggagcagctc tgcctcctgc gcctgtcgaa tcagttcact
      181 ggctctcacg ctgcgctttc ctggttcgag atccatcatt gcctggcgtt tctgagcatc
      241 ctcgcgtgcg tctttcatag cggttcaatc aggggttgcc agagccggtg cgctccaggt
      301 ggccgtctgg gcgccggaca ctggcgcgcg ttcggcggcg atggccttag aagtagccct
      361 cccgcttctt ctgctccatc gagctgtcct ggtcgtggtc gatgaggcgc gtgatccgct
      421 cggattgccg gaccaccatc atctcctcga tgatttcctc tactgtgcgc gcggaagagc
      481 ggattgctcc ggtgcaagga tagcacccga gagtccggca ccggcagagg accttctccg
      541 gcttctcgcc cggaaggaga cgggtgcgct cgtagaccgg aatcagccgg ccctcgcgca
      601 cgacaacctc gcgctcggcg gcaaagtaca tcggcacgac gggaatctgc tcgcggtgga
      661 tatagagcca gatatcgagt tcggtccagt tcgagagggg gaaggcgcgg atgctctcgc
      721 cgcgggcgat ccggccgttg tagaggttcc atagctccgg gcgttggttt ttcgggtccc
      781 attgcccaaa ggtatcgcgg aaggagtaga cgcgttcttt cgctcgcgag cgttcctcct
      841 cgcggcgcgc cccgccgaag gcggcgtcgt agcccccctc cttgagggca ttcagcagcg
      901 cctgggtctt caaggcggcg cagcaacgca cggtgcccca atcccagggg ttgacgccct
      961 gcgcgattgc ctcctcgtag cggtagaccc tcaactccgc gccgatggac cggcagaatt
     1021 cgtcacggaa ggagtacatt tccggaaact tcaggccggt atcgacgtgc aggagaggga
     1081 atgggatcgg cccgggtgcg aacgctttct gcgccagacg aaccatgacc gaggagtcct
     1141 tgcccaccga atagagcatc accgggcgct cgaactcggc gactacctcg cggatgatat
     1201 ggatactctc cgcctccagc gcgtcgagcg tgttcaggcg aagcgcttca gtatgtatcg
     1261 tactcatact gccgtacccc tccggcagtg ctattataac agcgcgcggt ttcggcagcc
     1321 ttcacgatgc ggccaaatag tagcactatc gggccaattc gacgccggac gccgccgggc
     1381 cttcccaatg ggcatcggaa agcaagcgcg caaatcagtg gccgtgccct ctggcacttg
     1441 acgcccctct caggaatggc gtatgatccg aagagaccgg gcgctcaggt tgcccggtgg
     1501 agagatcctc gaggaggcga ttgtggaagc gctcgtgccg ccgatccgaa ggcttacccg
     1561 cgccgaagtc ttcagccata gccatctcgc tttggacctg aagcggatcc atagccaccc
     1621 gagcaccggc gtccacaccc acgagttctc ggagctggtg gtggtgttgc gtggaacagg
     1681 cattcacgtc acgccgggcg gcgagtaccc catcggcgcg ggcgatgcct tcgttctcca
     1741 tggcgatgag gcccacggct acatgaacac cgaggacctc gacctggtga atatcctctt
     1801 cagcctggag gagctgggcc tgccgcgcgc cgatatcagc gcattgcccg gcttccacgt
     1861 gcttttcacg ctcgagccga agtaccgccg ccgcgaccgt tttgagagcc ggttgcgcct
     1921 cagcccggag aagctccacc acgtcgccgg cctgatcgat ggcctggaga aggagctggt
     1981 gcgccgcgat ccgggatggg cgttcgcggc gaccgcctac ttcatgctga ttgtgtcgga
     2041 cctgagtcgg ttctactcgc atgccgagga gccggcggtg cagcccctac ttcgccttgg
     2101 gcgcgtcgtc agccacatgg agcgtcactt tgccgatgcc gtgagcctgg acgatctggc
     2161 tcgcgtcggt tgcctgtcgc ggcgcacgct cacgcgcgtc tttcggaacg ccttcgggtg
     2221 ctcgccgatc gagtatctca cccggctgcg cattaaccac gccatggccc tgctccagga
     2281 tgccgatctg agcatcaccg agatcgcttt ccaggtgggg ttccgagata gcaaccactt
     2341 ctcccgtcgc tttcgt
//
LOCUS       DAOSCV010000730         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29618_length_2355_cov_3.370435, whole genome shotgun sequence.
ACCESSION   DAOSCV010000730 DAOSCV010000000
VERSION     DAOSCV010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29618_length_2355_cov_3.370435"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLY56_15545"
     CDS             <1..430
                     /locus_tag="PLY56_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22941.1"
                     /translation="AATIDTFLKRPVHSLSEFLAQVRRDEVVQKRYGKHLGIAWQEVP
                     RYLERRLKPATIMKTGFFTVYNVTTEGAIFPTKQRLTAGTRVYRLQGSPHFFTQRGEP
                     ARPFFKPTVERMVPDPKQSQTQAPPSEPVVQVEVVSVPSE"
     gene            472..1371
                     /locus_tag="PLY56_15550"
     CDS             472..1371
                     /locus_tag="PLY56_15550"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOJ22942.1"
                     /translation="MAETVEEQRRQGEEGNKQRPTAWVHLALGVLGALLLLWYRDVLQ
                     FWRVEWTGNAGYYSHGPLVPVLAAILIWMRREQLSRAPSAPSYLGLVAVAAGGALKLG
                     STWVSTTGVEAFASFSFPIVLWGLILGLYGGAIARILAGPVAFLWLMCPLPGALITEL
                     SFPLQLASTRLGVGTASILGVNVHQEGTAIHLETLSLFVGEACSGFRSTLGLMMLSAF
                     AAVSSRLGAGKKLLLLLSALPIGLAANGFRIAAITWAAERWGREAAIRAHDTSGLVVL
                     ALACVAVAATARILGWLDDTDTW"
     gene            1344..2033
                     /locus_tag="PLY56_15555"
     CDS             1344..2033
                     /locus_tag="PLY56_15555"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOJ22943.1"
                     /translation="MAGRHRYLVALALAAALAGLWQADRHVLRAKQSATPREMLWVPE
                     QVGAWRGEEIAVRLEDAALLPRTEIAMRRYQIPGEPQALAVDLAAVSARDLSAFHNPD
                     SCYRAAGWTIVDKQPLKIDTGDPDAPILMERLLVEKDGVRVHVGYWYMTAGGLAGGPE
                     RTHTSRLGVLLDALRWGGTRPATFFRVTTYATGGEIEAELRSAHFIRALLQSFRGNER
                     GKNTEERRHTG"
     gene            2030..>2355
                     /locus_tag="PLY56_15560"
     CDS             2030..>2355
                     /locus_tag="PLY56_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22944.1"
                     /translation="MRHYLLAALLAVTVAAGCGGDATSMTHTPPDTSTHPVDIGIGRQ
                     FNVPSAPSGAGIETYSNAVFNFSLQFPADEFALFDPPQYVTTLGNRVFELVSRTLPEG
                     EVFRPNV"
BASE COUNT          382 a          741 c          814 g          418 t
ORIGIN      
        1 ggccgccacg attgacacct ttctgaagcg gcccgtgcat tcgctgagcg agttcctggc
       61 tcaggtgcgc cgcgatgagg tggtgcagaa gcggtatggc aaacacctcg ggatcgcctg
      121 gcaggaggtg cccagatatc tggaacgaag gctcaagccc gcgaccatca tgaagacggg
      181 cttcttcacg gtttataacg tgacgacgga gggcgccatc tttcctacga agcagcgcct
      241 gaccgcggga acccgcgtct accggctgca gggctcgcct catttcttca cccagcgtgg
      301 agaaccggcg cggcccttct tcaagccgac ggtagagcgg atggttccag atccgaagca
      361 gtcgcagacc caggcgccgc cctccgagcc cgtggtgcag gttgaggtcg tgagcgtgcc
      421 ttccgagtga gtggcaggcc cgacccgatg gggggctgag gggagctggg agtggctgag
      481 accgtagaag agcaacgcag acagggagag gaaggcaaca agcagcggcc gacggcgtgg
      541 gtgcatctgg cattgggagt gctgggggcg ctcttgctcc tctggtaccg cgatgtgctg
      601 cagttttggc gcgtcgaatg gaccggcaat gccggatact actcgcacgg cccgctggtg
      661 ccggtgctcg ccgccatact catctggatg cgccgcgagc aactcagccg ggctcccagc
      721 gccccctctt atctgggcct ggtggccgtt gccgccggag gagcgctgaa gctaggctcg
      781 acctgggttt ccacgaccgg ggtggaggct ttcgcgagtt tctccttccc catcgtgctc
      841 tggggcttga tcctgggcct gtacgggggc gccatcgcac ggattctcgc gggcccagtg
      901 gcgttcctct ggctgatgtg tcccttgccc ggggcgttga tcaccgagct gagcttcccc
      961 cttcagctgg cgtctacgcg cctgggagtg gggacggcta gcatcctggg tgtgaacgtt
     1021 caccaggagg ggacggcaat ccacctggag acgctctccc tcttcgtggg ggaggcgtgc
     1081 agcggcttcc gatccaccct gggcctgatg atgctctcag cgtttgccgc agtgtcttcg
     1141 cgcctgggag cggggaagaa gctgctgctc ctcctatcgg cgctccctat cggcctggca
     1201 gccaacggct tccggatagc ggcgatcacg tgggctgccg aacgttgggg ccgagaagcg
     1261 gcgatacggg cgcacgacac ctctggtctc gtcgtgctcg cgcttgcctg cgtggccgtc
     1321 gcggccaccg cgaggatatt aggatggctg gacgacaccg atacctggta gccctggcac
     1381 tggccgccgc cctcgccggg ctgtggcagg cggaccgcca cgtgctaagg gccaagcaat
     1441 ccgccacgcc tcgggagatg ctctgggtgc cagagcaagt gggtgcctgg cgcggggagg
     1501 agatcgccgt gcgtctcgag gacgcggcct tgttgccgcg caccgagatc gcgatgcgcc
     1561 gctatcagat ccccggagag ccccaggcgc tcgcggtaga cctggcggcg gttagcgcgc
     1621 gcgatctcag cgcgttccac aaccccgact cctgctaccg ggctgcaggg tggaccatcg
     1681 tggacaaaca gccgctgaag atcgatacgg gagacccaga cgcgcccatc ctgatggagc
     1741 ggctgcttgt cgagaaagat ggcgtgcggg tgcatgttgg ttactggtat atgacggccg
     1801 gcgggctcgc aggcggccct gagcggacgc acacgagccg gctcggcgtg cttctagatg
     1861 ccctgcggtg ggggggcacg cggcccgcga cgttcttccg cgttacgacc tatgccaccg
     1921 gtggggagat cgaggcggag ctccgcagcg cgcacttcat ccgggcgttg ctgcagtcct
     1981 tccgcggcaa tgagaggggt aagaacaccg aggagaggag acacacagga tgaggcacta
     2041 cctgctcgcc gcgcttctgg ccgttacggt cgcggccggg tgcggcggcg atgcaacatc
     2101 catgacgcat acaccccccg atacttccac gcaccccgtg gatatcggga tcggccggca
     2161 gttcaacgtg ccgtcggcac cgtcaggggc tgggatcgag acctatagca acgcggtctt
     2221 caacttcagc ctccagttcc cggcagatga gttcgcgctc ttcgacccgc cgcagtacgt
     2281 gaccacgctc ggcaaccgcg tcttcgagct ggtgtcacgg acgctgcccg agggggaggt
     2341 gttccgccca aatgt
//
LOCUS       DAOSCV010000731         2352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29681_length_2352_cov_3.747932, whole genome shotgun sequence.
ACCESSION   DAOSCV010000731 DAOSCV010000000
VERSION     DAOSCV010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29681_length_2352_cov_3.747932"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2352)
                     /locus_tag="PLY56_15565"
     CDS             complement(<1..>2352)
                     /locus_tag="PLY56_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22945.1"
                     /translation="NRGTRYNPKTDGKRNDCYDRFFITLSPFYEEMLPSIPNPESPWK
                     HITGTRLWRAHGASNREHDARYWTECHRWGMTQVVVTDHETGWRDGGESFTFRTKPAP
                     GKGGDKGQYDYARLMQDTLGFVYGPYNNFTDFAPVNEYWHTDMVSRTSDNQLQHAWMR
                     CYAPKPARAVEFCERLAPQIQEKFRFSTAYCDVHTAVAPWTRVDYDARVPGAGTMAAT
                     FYAFGEIMLLQKKAWNGPVYSEGNHHSFYSGLTDGNYAQDQNYRIAENPWLVDFDLRK
                     MHDLCCNFGMGSPSMFYTQEGFRTMREVDADTWLDRFLAATVAFGHPGFLTFEGGIQN
                     ALRSYYMLQQLHSRYCLESASEIRYADANGQLLNTSQAVASGAYKRSQVVTRYSKGTV
                     TVVNGSPTERMVVTAYGRKLDLPPNGYCGWTADGAIEVISSDPGGRRCDYAVTPAYIY
                     VDGRGHFARFPKAGGNGIGICRILGKGQYEVLLHKDADCGFAIQASSAVALDRERKPI
                     GPAKLRTSRGLTYVIPVEGAFSYLLTAGKPAAPVALSSDRAEVFAGERVVVRGKQRHE
                     VLIPSDAVPGQRIWVQKEGSWIDFTVVPLVEADATLAGNTVRVTLTSNMPRPAQFLVK
                     MAGKEQRVRLEPRTPGTVSVDLGQPAEESADVLAIEVSTGELSQRFERGLCTLRAVAP
                     LVELPEKWTAGMCLRGGKETTDFGQTRAAVSDRDLTSGEVIKRAIFMHPPYIGGTGYC
                     FALYDPVTLPATPAAAFRAVVGKGDGSYDGDGILYKLVVVDEA"
BASE COUNT          409 a          759 c          755 g          429 t
ORIGIN      
        1 cggcctcatc gacgactacc agcttgtaga ggatgccatc gccgtcatag ctgccgtcgc
       61 ccttgcccac caccgcgcgg aaagcagccg cgggcgtggc tggaagcgtc accggatcgt
      121 agagggcaaa gcagtatccc gtgccaccga tatagggtgg gtgcataaag attgctctct
      181 tgatcacctc tccagaggtg agatcccggt cggaaacggc ggcccttgtc tgcccaaagt
      241 cagtcgtctc cttgccgccg cgaaggcaca tgccggcagt ccacttctcc ggaagctcca
      301 ccagcggcgc cacggcgcgc agcgtgcata gtccgcgctc gaagcgctgg ctcagctcgc
      361 cagtgcttac ctcaatcgcc agcacatccg ccgattcttc ggccggctgc ccaagatcga
      421 cgctgacggt tccaggcgtg cgcggctcca ggcggactct ctgctcctta cccgccatct
      481 tcacgaggaa ctgcgcaggc cgcggcatgt tgctcgtcag ggtgacgcgc acggtgttcc
      541 ccgccagtgt cgcgtccgcc tcaaccaggg gcacgacggt gaagtcgatc cagctgcctt
      601 ccttctgcac ccagatccgc tggcccggaa cggcatcgga cgggatcagc acctcgtggc
      661 gctgctttcc gcgcaccacc acgcgctcgc cagcgaagac ctccgcccgg tcgctcgaaa
      721 gcgccacggg cgccgccggc ttgcccgcgg tgaggaggta gctgaaggca ccctccacgg
      781 gaatcacgta ggtgaggccg cgcgacgtgc gcagcttcgc cggcccgatc ggcttgcgct
      841 ctcggtcgag cgccacggcg ctcgatgcct gaatagcaaa gccgcaatcg gcatccttgt
      901 ggaggagaac ctcatactgg cccttaccca gaatccggca gatgccgatg ccgttcccac
      961 ctgccttcgg gaagcgcgcg aaatggccgc ggccatccac gtagatgtag gcgggggtca
     1021 cggcgtagtc acagcggcgc ccgcctggat cgctgctgat cacttcgatc gcgccatcgg
     1081 cggtccaccc gcaatagccg ttgggcggga ggtccagctt gcggccataa gccgtaacga
     1141 ccatgcgctc ggtggggctg ccgttcacca cggtgacggt gcccttcgag tagcgcgtga
     1201 cgacctggga gcgcttgtag gcgcccgagg cgaccgcctg agaggtgttc agcagctggc
     1261 cgtttgcgtc ggcatagcgg atctcggaag cgctctccag gcagtagcgg ctatgcagct
     1321 gctggagcat gtagtagctg cgaagcgcgt tctggatgcc gccctcgaag gtgaggaagc
     1381 cggggtggcc aaacgccacc gtggccgcca ggaagcggtc gagccaggta tccgcatcaa
     1441 cctctcgcat cgttcggaag ccctcctggg tatagaacat cgaggggctt cccatcccaa
     1501 agttgcagca gaggtcgtgc atcttgcgga gatcgaagtc caccagccag gggttctcgg
     1561 cgatgcggta gttctggtcc tgggcgtagt tgccatcggt gaggccacta tagaaggagt
     1621 ggtggttccc ctcggaatag acggggccgt tccatgcctt cttctggagg agcatgatct
     1681 cgccaaaggc gtagaaagtg gccgccatgg tgcccgcccc gggcacgcga gcatcgtagt
     1741 ccacgcgggt ccagggggcc acggcggtat gcacgtcgca gtaagcggtg ctaaaccgga
     1801 acttctcctg gatctgcggc gccagccgct cgcaaaactc cacagcacgc gccggcttgg
     1861 gcgcgtagca gcgcatccat gcgtgctgga gctggttatc ggaagtccgg ctcaccatat
     1921 cggtgtgcca gtactcattc accggtgcga agtcggtgaa gttgttgtag gggccgtaga
     1981 cgaatcccag ggtatcctgc atcaggcggg cgtaatcata ctggccctta tcgcccccct
     2041 tgccaggcgc gggtttcgtg cggaaggtga aactctcgcc gccatcgcgc caccccgtct
     2101 catggtcggt caccaccacc tgagtcatcc cccagcggtg acattccgtc cagtagcgcg
     2161 cgtcatgctc acggttgctg gcgccgtggg cccgccaaag gcgcgttcca gtgatgtgct
     2221 tccagggcga ttctggattg gggatcgagg gaagcatctc ctcgtagaag ggcgacaggg
     2281 tgatgaagaa gcggtcgtag cagtcgttgc gcttgccatc cgtcttggga ttgtagcgcg
     2341 tcccacggtt gt
//
LOCUS       DAOSCV010000732         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29745_length_2349_cov_3.333043, whole genome shotgun sequence.
ACCESSION   DAOSCV010000732 DAOSCV010000000
VERSION     DAOSCV010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29745_length_2349_cov_3.333043"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLY56_15570"
     CDS             complement(<1..615)
                     /locus_tag="PLY56_15570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ22946.1"
                     /translation="MMRTDGRRPSATPRMAAWRSFLVLPLFFLSPAARIHVDGMMRDV
                     TYLASTIGPRVAGSAEERRAALYILSRFDSLGYPARLQEFTLPNGRRSANVIAGHAAE
                     ALRVVVGAHYDSRPNAPGADDNASGVSVLLELARQMAGKRRDLLFVAFGAEERIDRNR
                     SHHHYGSRHFVQAASTGLLQGLSGMLCIDCVGSGPHFYVQGMRND"
     gene            complement(792..1556)
                     /locus_tag="PLY56_15575"
     CDS             complement(792..1556)
                     /locus_tag="PLY56_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22947.1"
                     /translation="MRVSVLTGGILAAALVLASPAGAQRGPGEEAGAEERFAPVGLVL
                     NPTAYTLPAGRWLIGAGTFQQAVVTGDSDRQYSAGIFYGISDNLYVGGFWTGVQRDRP
                     DGSDIVESYFGGLAQYKLISETAGSLVPSFSIGGYAYTGPGEGGTLYAVASKNLMGRV
                     RPGAVFLHAGVRWDTFDNDGLDDDAIRPFVGANLLVTRELALNWEWRQRHFGETNNLW
                     AAGAIWLINNRYALSAGVQDPGSGSTQFYAGLSIVP"
     gene            2203..2349
                     /locus_tag="PLY56_15580"
                     /pseudo
     CDS             2203..2349
                     /locus_tag="PLY56_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
BASE COUNT          402 a          789 c          759 g          399 t
ORIGIN      
        1 atcgttgcgc atcccctgca cgtagaaatg aggcccgctt ccgacgcagt cgatgcagag
       61 catgccgctc agcccctgga gcagcccggt cgacgccgcc tgcacaaagt gacgcgagcc
      121 gtaatggtgg tgcgagcgat tccggtcgat ccgctcttct gctccgaagg cgacgaagag
      181 cagatcgcgc cgcttgccgg ccatctgtcg ggcaagctcc aggagaactg agacgccgga
      241 ggcgttgtca tcagcgccag gcgcatttgg gcgcgagtcg tagtgagccc cgaccaccac
      301 gcgcagcgcc tctgcggcat gaccggcaat cacattcgcc gagcgacggc cgttgggcag
      361 cgtgaactcc tgcaggcgcg ccggatagcc cagggagtcg aaacgtgaca ggatgtacag
      421 tgccgcccgt ctctcttcgg cgctgccggc cactctcggg ccgatggtgg acgcgaggta
      481 ggtcacgtcg cgcatcatgc cgtcgacgtg aatcctggcc gcgggggaga ggaagaacag
      541 cgggaggacc aggaaggagc gccatgctgc catccgaggc gtggcactgg gccgccgccc
      601 atccgtgcgc atcatcacgc ttcatctcct ctcgcgtccg tgggggaaac acccgggcag
      661 gtgcccggaa gcgcggcgta tcgctcacgc tacctgcgtg gccccacgca gaatggccgg
      721 ctccgaggag agagcagggc gtcgccaccc gttcaccgcc ggagccggca cgttccgcgc
      781 gtcccatcgc gctaggggac aatgctcagc ccggcgtaga actgcgtgga tcccgacccc
      841 gggtcctgaa caccggccga gagcgcatac cggttgttga tgagccagat ggcacctgcc
      901 gcccagaggt tgttggtctc gccgaagtgg cgctgtcgcc actcccagtt gagcgccagt
      961 tcgcgtgtca ccagcaggtt cgcgccgacg aacggccgga tcgcgtcgtc atcgaggccg
     1021 tcgttgtcga aggtatccca gcgcacacca gcgtgaagga acaccgctcc tgggcgcacc
     1081 cggcccatca agttcttgct tgcgactgcg tagagcgtgc cgccttcccc cgggccggtg
     1141 taggcatacc caccgatcga gaaggatgga accaaggagc cggccgtttc gctgatcagc
     1201 ttgtactgcg ccaggcctcc gaagtagctc tccacgatat cggagccatc cggccggtcg
     1261 cgctgcacgc ccgtccagaa accacccacg tacagattgt cgctgatccc gtagaagatg
     1321 cccgccgagt actgcctatc gctgtcgccc gtcacgacag cctgctggaa agtacccgcc
     1381 ccgatcagcc accggcctgc tggaagcgtg taggccgtcg ggttgagcac caatccgact
     1441 ggggcaaagc gctcctcggc gcccgcctcc tcgccgggac ctcgctgggc accggccgga
     1501 gacgccagga ccaacgccgc ggcaagaata ccgcctgtga gaaccgaaac gcgcattcct
     1561 cttcacctcc ggcccgcgat tgcgcaattt cagtgccaga ggcaggagag taggggggcg
     1621 tcgtagcgtc cgggcgcagc aacctggatt tgctctcccc ggcatcaggt gagggtggcg
     1681 cggcagcggg catgcgcggg caaaatctgc caccacacgg gagcgttcat ggcgtttgga
     1741 tgcctgcggg tcggggtaaa ctcctcatcg gtacactgct attctgccgc gaacctgacc
     1801 caggtaggga ttcggggaga cccttgctga cgaccggacg cctgctttct gggcaccagc
     1861 actggccgtt catctctgct gcaagggccc gcatccactg agcgaggctg tttctcggtc
     1921 ggctggctgt cggagtccgc tcgcgggcac tggcgtcaag cgtgtacccc aatgccgggg
     1981 ccaggggtga ggtcgccgtt gagtcgcacg catatctatc cacttgagcc ggagctgtcg
     2041 aaggcttcgg agaggcttcc gggggcgctt cttgctaggg ctcgtcgtca tccacgcctg
     2101 gcccttctct tttcgctcct ctgcacgctg gccgtcagtg cgctccacat ggcgtcagga
     2161 tcggcacggc atgccacatg cctctacgtt gttcccgtga tcagcggcgc ggccctgctg
     2221 cccatcgacg ccaacggcga tctgccggcc ggtggctggg agcaattgca ggccgccaag
     2281 atgatcgtct tcggctcgcc gacctacatg ggcacggtca gctggcagtt caagaagttc
     2341 gccgacgcc
//
LOCUS       DAOSCV010000733         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29793_length_2346_cov_2.555216, whole genome shotgun sequence.
ACCESSION   DAOSCV010000733 DAOSCV010000000
VERSION     DAOSCV010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29793_length_2346_cov_2.555216"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="recN"
                     /locus_tag="PLY56_15585"
     CDS             complement(<1..1337)
                     /gene="recN"
                     /locus_tag="PLY56_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ22948.1"
                     /translation="MLSELHITDFALVDELSVRFGPGLNVLTGETGAGKSIIIDAISA
                     VLGMRADYDLIRTGAQRAVVQAAFDLSDAPEARAAAAEAGIESEEDLLLVTRELQASG
                     KSQCRINGRLATVSMLRALGHTLIDVHGQHEHQSLLSVSRHLDLLDAWAGPEVMELRA
                     RFAERFRALTSLDEQIEKLTTDARDRARKVDLYQFQIQEIDAAGLRDGEEEELEAERL
                     RLANAEKLFLNASQAYDRLRGDDALLDSLAACQELLEGLAEVDRSASSLVEPVASALY
                     QLEDVAKELRTYRDSIEFNPERLEEVQERLIAIQHLKRKYGDTIAEILAYRKQLAAEL
                     DGLTHSEEREAELRQQREAVHAEITSLAERLTQLRYEATGRFQDEVETELAALAMEKT
                     SFRVALEIGALGPTGTDRVEFLISPNPGEPLKPLALIASGGELSRVMLALKSIL"
     gene            1648..>2346
                     /locus_tag="PLY56_15590"
     CDS             1648..>2346
                     /locus_tag="PLY56_15590"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ22949.1"
                     /translation="MRTPRLWDRIEGMLLGLAIGDSLGNTTEGLLRDQRAQRYGTIRD
                     YLPNPRAENRPVGLPSEYTQLAMWTLEQLLADKGLVPDHLARRFCQQWIFRVSRTLRE
                     FIRRYKDEQVPWERAGVASAGNGALARIAPILVPHLQQPSPALWADVALAAMLIHNDP
                     ASIGSCLALERLLWEVMGLSSVPDPRWWVDHFCSTLHPLEGEATYETRSPHVPYRGPL
                     WLFVEASVPRAVSDG"
BASE COUNT          364 a          784 c          774 g          424 t
ORIGIN      
        1 aggatcgact tgagcgcgag catgacgcgc gacaactcgc ctccagaggc gattagcgcc
       61 aatggcttca gcggctcgcc cgggttaggc gagatcagga actcgacgcg gtcggtcccg
      121 gtggggccca gcgcgcctat ctcaagcgcc acgcggaagc tcgtcttctc catcgcgagc
      181 gcggcgagct ctgtctctac ctcgtcctga aaccgtcccg tcgcctcgta ccgcagctgg
      241 gtgagccgtt ccgcgaggga tgtgatctcg gcgtggacgg cctcgcgctg ctggcgcagc
      301 tccgcttcgc gctcctcgga gtgggtcagc ccatccagct ccgccgcgag ttgcttgcga
      361 taggcgagga tctccgcgat cgtatcgcca tacttgcgct tcaggtgctg gatcgcgatg
      421 aggcgctcct ggacctcctc caggcgctcc gggttgaatt cgatgctgtc gcgataggtg
      481 cggagctcct tcgcgacatc ctccagctgg tagagcgccg atgcgaccgg ctcgacgagg
      541 gaggaagcgg agcggtcgac ctcggccaga ccctccagga gctcctggca cgcggctaga
      601 ctatcgagga gcgcgtcgtc gccacggagc cggtcatagg cctggctcgc gtttaggaac
      661 agtttctcgg cgttggcgag ccgcaggcgc tccgcttcca gctcctcttc ctcgccatcg
      721 cggaggccag cggcatcgat ctcctggatc tggaactggt agaggtccac cttccgcgcc
      781 cggtcgcgcg cgtcggtggt cagcttctcg atctgctcgt cgagcgaggt cagcgcgcga
      841 aagcgttcgg cgaagcgcgc gcgcagctcc atcacctcgg ggcccgccca ggcatcgagc
      901 aggtccagat ggcgcgatac cgaaaggagc gattggtgct catgctggcc atgcacatcg
      961 atcagggtgt gcccgagagc gcgcagcatg gagacggttg ccaggcgacc attgatacgg
     1021 cactggcttt tgccgctggc ctgaagctcg cgggtgacca ggagcagatc ctcttcgctc
     1081 tcgattccgg cttccgccgc agccgcccgg gcctcgggcg cgtccgacag atcgaaggct
     1141 gcctggacga cggcgcgctg ggcccccgtg cggatgagat cgtagtcggc gcgcattccc
     1201 aggacggcac tgatggcgtc gataatgatc gatttgcccg cgcctgtctc cccggtcaat
     1261 acgttcagcc cggggccgaa acggacactc aattcatcaa ccagtgcaaa gtccgtgatg
     1321 tgcagttcgg atagcatagg tggatccacg cggccgtagg ccgccctgtc tattctacca
     1381 ttcgcaagcg tttcacgcaa cggcagggac gtggctctct cccatagagg ggggtcgcgc
     1441 gcgcagcgag aatgcatcca gaaggctttc ggcgaagagg ccgctcttct ggtgatgcca
     1501 gccaccggcg ggcgccagga cgaggcgtcg gtcgagtggg cgcctggaag gagacaagat
     1561 ggataaccgc cccgtgctag agcgcttgtt tgcgtccggc gggattgccc tccgacgtag
     1621 cccgctgctc gagagccccc cgccccgttg cgcactcccc ggctgtggga ccggattgag
     1681 ggcatgctcc tcgggctcgc cattggcgac agcctgggca acaccacgga gggcttgctg
     1741 cgggaccaac gcgcccagcg gtatggcacc atccgcgatt acctgccgaa tccccgcgcg
     1801 gagaaccgcc cggttggcct tccctcggag tacactcagc tggcgatgtg gaccctggag
     1861 cagcttctgg cggataaggg cctggtaccc gaccacctgg cgcgccgctt ctgccagcag
     1921 tggatcttcc gcgtgagccg cactctccgc gagttcatcc gccgctataa ggatgagcag
     1981 gttccctggg agagggcggg ggtagcgtcc gcggggaatg gcgcgctcgc gcggatcgcc
     2041 cccatcctgg tgccccacct gcagcagcct tcgccggcgc tatgggccga tgtcgccctc
     2101 gctgccatgc tcattcacaa cgacccggcc tctatcggct cctgcctggc cctggaacgg
     2161 ctcctctggg aagtgatggg gctctcatcc gtcccggatc ccaggtggtg ggttgatcac
     2221 ttctgctcca cgctccaccc cctggagggc gaggcgactt acgagacgcg ctcgccgcat
     2281 gtgccttatc gggggcccct ctggctcttt gtcgaggcga gcgtcccgcg ggcagtcagc
     2341 gatggc
//
LOCUS       DAOSCV010000734         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29823_length_2344_cov_3.079074, whole genome shotgun sequence.
ACCESSION   DAOSCV010000734 DAOSCV010000000
VERSION     DAOSCV010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29823_length_2344_cov_3.079074"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLY56_15595"
     CDS             complement(<1..700)
                     /locus_tag="PLY56_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ22950.1"
                     /translation="MYVPRNPGLPPRALFTPPGGMSGALDCAGLLLFSKARMGIRYAL
                     SALGIGLGETVLAPAYLTGSALAPFDWAGVACALYDVDRTTLAPDWGSVAGVQTRVKA
                     RALLLVHYFGLPCDGGIARAFCREHGLALIEDCAHALPPAPGEVGPGALGDAAIFSLR
                     KLLPLPDGGALRLGSGRRAPACLPPRSDSPVALAPLLARYVAQRLGMHRPSRAARPPL
                     PVPDTRPKEPAVGIS"
     gene            complement(723..1625)
                     /locus_tag="PLY56_15600"
     CDS             complement(723..1625)
                     /locus_tag="PLY56_15600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ22951.1"
                     /translation="MKVSVITCARYRHEGIGRLVRSIRESTHPDFELLIVDQSGGEIE
                     TEVLAAAEGDRRVRFLRLNEPVRSKAMNHGVRQSTGAVLAFTNDLCEPRPDWLEVLAR
                     LFCQTTADIICGKVSAPPYDVSTGYICEFDPGRPYLVTNQWCSLRQLGVGANMAMRRS
                     VFARVGYFDECIGPGGIIPSGDDRDFLYRAVRVGCRVLVTPDPEVIHHDYRSWEEDGR
                     DAGRSHSIGLAVPYAKFMRRRDPAAFYHYLSDFLGDARHAASRVARMRRPLGINRLRY
                     YLEGAAQGFKPPLDPGTMVFRKHS"
     gene            complement(1622..>2344)
                     /locus_tag="PLY56_15605"
     CDS             complement(1622..>2344)
                     /locus_tag="PLY56_15605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ22952.1"
                     /translation="HEAGGDVVAVIDDDCEARPDWIAALVAELENGGADIVCGQVEPG
                     PHNPAEGYVVSFQPRQPALLKSHWCSVHHLGRGNNMAFLRSVLRRVGPFDELLGPGAP
                     IPTGEDRDFLYRALRKRCLVKLSPAPVVVHHDFRPWDQSDGPNGMTASYAAASAYWKH
                     IRGGDLVALLWYLIDVADDTRYAAGRVLRGQRPLGLNRLRHLLAGAAHSMRHPVNRQM
                     GVYRQGPERGEGGQRRQEAGRP"
BASE COUNT          426 a          817 c          752 g          349 t
ORIGIN      
        1 ggctgatccc caccgccggc tcctttgggc gcgtatcggg aacggggagc ggtggccggg
       61 cggcgcgcga gggccggtgc atgcccagac gctgtgccac gtatcgggcg agaagcggag
      121 cgagcgccac cggagaatcg ctgcgcggag gcaggcacgc gggcgccctc ctgcccgaac
      181 ccaggcggag ggcgccgcca tccgggagag ggagcagctt tctcagggag aagatcgcgg
      241 cgtcgcccag agcgccgggt cccacttccc ctggcgcagg gggcagcgcg tgcgcgcagt
      301 cctcgatgag cgccaggcca tgctccctgc agaaggcgcg agctatccca ccatcacacg
      361 gaagtccgaa gtagtggacg agcaggagcg cgcgcgcctt gacgcgcgtc tgcaccccgg
      421 ccacgcttcc ccagtcgggc gccagggtgg tccggtccac gtcatacagc gcgcacgcca
      481 cgcccgccca atcgaacggc gcgagagcgc tcccggtgag gtaggccggc gccagaaccg
      541 tctcccccag cccgatccca agggcagata gggcataccg gatacccatg cgcgcctttg
      601 aaaagaggag cagtccggcg caatcgagcg cgccgctcat cccacccgga ggcgtgaaga
      661 gcgcgcgtgg cggcaatcct gggtttctgg gcacgtacat cgcgggtagt ctcgtgcgcc
      721 ccttacgagt gcttgcggaa gaccatcgtg cccggatcga gcgggggctt gaagccctgc
      781 gcggcaccct ccaggtagta gcggaggcgg ttgatcccta gcgggcggcg catgcgcgcg
      841 acccggctgg cggcatggcg cgcatcgccc agaaagtcac tcaggtaatg gtagaaggcg
      901 gcgggatcgc ggcggcgcat gaacttcgca tagggaaccg ccagcccgat gctgtgggat
      961 cgcccggcat cccgcccgtc ctcttcccac gagcggtaat catggtggat cacctcaggg
     1021 tccggcgtca ccagcacccg acagccgacc cggaccgccc ggtagaggaa gtcacggtca
     1081 tccccagacg ggatgattcc gccgggccca atgcactcat cgaagtagcc gacacgcgcg
     1141 aaaacactcc ggcgcatcgc catgttggcg ccaactccca attgacgcag gctgcaccac
     1201 tggttcgtca ccaggtaggg gcggcccgga tcgaactcgc agatgtagcc cgtggatacg
     1261 tcataagggg gagcactcac tttgccacag ataatgtcag cggtcgtctg gcaaaagaga
     1321 cgcgcgagga cctccagcca atccggccgt ggctcgcaca gatcattcgt gaaggccagg
     1381 accgcgccgg tgctctggcg cacgccgtgg ttcatcgcct tgctcctcac cggctcattc
     1441 aggcgaagga agcgaacccg gcggtcgccc tcggcggcgg ccaacacctc ggtctctatc
     1501 tcgccgccgc tctgatccac gatcaggagc tcaaaatcgg gatgggtgct ctcgcggatg
     1561 ctccgcacca gcctgccgat cccctcatga cggtagcgcg cgcacgtgat gaccgatacc
     1621 ttcatggtct tcctgcctct tgcctcctct ggcctccctc tccacgctcg gggccctggc
     1681 gatagacgcc catctgccgg ttaacggggt gacgcatgct gtgtgccgcg cccgcgagca
     1741 aatgccgcaa tcgattcaac cccagcgggc gttgcccgcg cagcacgcgc ccggcggcat
     1801 accgcgtgtc gtcggcaaca tcgatcagat accagagtaa cgccaccaga tcgccgccgc
     1861 ggatatgctt ccagtacgcg gatgcggccg cgtatgaagc ggtcattccg ttggggccat
     1921 cagactggtc ccacggccgg aagtcgtgat gtaccaccac cggagcaggc gatagcttca
     1981 ccaaacaccg cttgcgcaaa gcccgataga ggaagtcgcg gtcttcgcca gtcgggatag
     2041 gagcaccagg accaagtagc tcgtcgaacg gccccactcg ccggagaaca ctgcgcagga
     2101 acgccatgtt gttgccccgg cccaggtggt gaacgctgca ccagtgcgac ttgagcagcg
     2161 caggctgcct cggctggaag ctaaccacat acccctcggc tggattgtgg ggccccggct
     2221 cgacctggcc gcagacaata tccgcgccgc cgttctccag ctcggccacc agcgccgcaa
     2281 tccaatccgg ccgggcctcg cagtcgtcat caatcaccgc cacgacgtcg cctcccgcct
     2341 cgtg
//
LOCUS       DAOSCV010000735         2342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29856_length_2342_cov_2.559248, whole genome shotgun sequence.
ACCESSION   DAOSCV010000735 DAOSCV010000000
VERSION     DAOSCV010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29856_length_2342_cov_2.559248"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLY56_15610"
     CDS             complement(<1..1446)
                     /locus_tag="PLY56_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ22953.1"
                     /translation="MRIDQIKLVRFGEFTDWEVGDLQPGLNALFGPNETGKSTLLGAI
                     RFLLFGSAGRQSGVNQYEPDGDKRHVEAILWHDGTLWHLTRRDVRGKGAFTLKPADGG
                     PNVLLDTFNRDWLGNTSRGTYQSVYALGLDDLQWCNELRDVILVQADPMGEKLRDAIK
                     ETEKATAQAPVKHALAAWKKANDEVLQLSQQPAQLNDYERQLSRERQRVAELDEELAG
                     VRKGLARCREILDARETWNRAASLRTQLEPLADVAEFPLTAERDYQAARERHDEARTQ
                     ADEARSRAQSIERELDGLWVDETLLTHAGAVEKLADEAAQHRELPGQLQEAEQRAEMA
                     LAAARQEADCRCGHDADIATLRNLDTSATTQSKLEEAASALSRAETAHAQAKKATDDA
                     ARAREQAEEEVAEVEKALDALPEPVDVDALRARVEQVERWQALQTEWRVARRDHETAQ
                     EKHDDLVARHKRESEARAQAEAQARRWEDDRA"
     gene            complement(1443..>2342)
                     /locus_tag="PLY56_15615"
     CDS             complement(1443..>2342)
                     /locus_tag="PLY56_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22954.1"
                     /translation="CSSPARPGGARRVLEATPLRVGLLHCDAGGTKNEENGYAPVSVG
                     DLARAPVDAWLLGHIHKRSEKPLHDRPLVIYPGNPQGRDINEAGPKGGLLVHLEPGRA
                     PKTEFVPFGSILWERVEVAVSECAGDTAVTEAIRDALQTRLDEASRTGAEGLIARVTL
                     AGRPELSTWRLLQSPETLDEIRKNAAGATETPFLVIEQLESRAQAPLPPREELRERED
                     LLGDFLRVLDQAREAPESGARRAVEEALSAFSKNADAREFGGIPGPEMPLEELLDAAE
                     KIALTLLWDEEDALASEEEPVQS"
BASE COUNT          301 a          808 c          778 g          455 t
ORIGIN      
        1 ggcgcggtcg tcttcccacc gccgcgcctg tgcctcggcc tgggcgcggg cctcactctc
       61 tcgcttgtga cgcgcgacca ggtcgtcatg cttttcctgg gcggtctcat gatcgcggcg
      121 cgccacacgc cactcggttt ggagcgcctg ccacctctca acctgctcga cgcgcgcgcg
      181 aagcgcgtcg acgtccaccg gctctggaag cgcgtcgagg gccttctcca cctcggcgac
      241 ttcctcctcc gcctgctcgc gcgcgcgtgc ggcgtcgtct gtcgcttttt tcgcctgggc
      301 gtgcgcggtt tccgcccggg agagggcaga tgcggcctct tccagtttgc tctgggttgt
      361 ggcggaagta tcgagattgc ggagagtagc gatgtcggca tcgtgcccgc acctgcagtc
      421 cgcttcctgc cgggcggctg cgagtgccat ctcggccctc tgctctgcct cttgcagctg
      481 gcctggcagt tctcggtgct gggctgcctc atcggcgagt ttctccaccg cgccggcgtg
      541 cgtaagcagc gtctcgtcta cccacagccc gtccaactcg cgctcgatgc tttgggcgcg
      601 cgaacgggcc tcatcagcct gcgttcgcgc ctcgtcgtgg cgctctctcg ccgcctgata
      661 gtcgcgttcg gcggtcaatg gaaactcggc cacgtcagcc agcggctcga gttgtgtgcg
      721 gaggctggcg gcccggttcc acgtctcgcg cgcgtccaga atctcccggc agcgtgccag
      781 gccctttctc acgccggcga gttcctcgtc cagttcggca acgcgctggc gctcgcgcga
      841 gagttggcgc tcgtagtcgt tgagctgcgc aggttgctgc gagagctgga gcacttcgtc
      901 gtttgccttc ttccacgctg cgagcgcgtg cttgacgggc gcctgcgcgg tggctttctc
      961 cgtctctttg atcgcgtcgc gcagcttctc gcccatcggg tcggcctgca cgaggatgac
     1021 gtcgcggagt tcgttgcacc attggagatc atccaggcca agggcgtaga cgctctggta
     1081 ggtaccacga gaggtgttcc ccagccagtc gcggttgaac gtgtccagga gaacgtttgg
     1141 accgccgtcc gcgggcttga gagtgaacgc gcccttgccg cgaacatcgc gccgggtgag
     1201 gtgccagagt gtgccgtcgt gccacaggat ggcttccacg tgccgtttat cgccatcggg
     1261 ctcgtactga ttgacgcccg actgcctgcc ggccgagcca aagaggagga aacggatggc
     1321 gccgagcagt gtgctctttc ccgtttcgtt cgggccgaag agggcgttga gcccgggctg
     1381 caggtcgccc acctcccaat cggtgaactc gccgaagcgc accagcttga tttggtcgat
     1441 cctcatgatt gtaccggctc ctcctccgag gcgagggcat cttcctcatc ccagagcagc
     1501 gtgagcgcga tcttctctgc tgcatccagg agctcctcaa gcggcatctc gggtccgggg
     1561 atgcctccga actcgcgcgc gtctgcgttc ttcgagaaag cgctgagcgc ctcttccacc
     1621 gcgcggcgtg cgccactctc gggggcttcc cgcgcctggt ccaggacgcg gaggaaatcg
     1681 ccgagcaggt cttcgcgctc ccgcagctct tcgcgcggcg ggagcggcgc ctgggcgcgc
     1741 gactccagct gctcgatcac caagaagggc gtctccgtcg cgccggcggc gttcttccgg
     1801 atttcgtcca gcgtttccgg gctctgtaac agccgccatg tcgaaagctc cgggcggccg
     1861 gcgagcgtta cccgggcgat caagccctcg gcgccggtgc gcgaggcctc gtccagccgg
     1921 gtctgaagcg cgtcgcggat cgcctccgtc acggcggtgt cgcccgcgca ttcggagacg
     1981 gcgacctcca cgcgctccca gagaatcgat ccgaacggca caaactcggt cttcggcgcg
     2041 cgccccggtt ccaggtggac cagcagcccg cccttcggcc cggcttcgtt gatgtcacgg
     2101 ccctggggat tgcccgggta gatgacgagc ggccggtcgt ggagcggctt ctcggagcgc
     2161 ttgtggatgt ggcccaggag ccaggcatcg accggggcgc gggccagatc gcctaccgaa
     2221 actggcgcgt agccgttctc ctcgttcttg gtgcccccgg catcgcagtg aagcagcccg
     2281 acgcgcaggg gggtcgcctc caacacccgg cgtgcgcctc caggccgagc aggggatgag
     2341 ca
//
LOCUS       DAOSCV010000736         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29893_length_2340_cov_2.992560, whole genome shotgun sequence.
ACCESSION   DAOSCV010000736 DAOSCV010000000
VERSION     DAOSCV010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29893_length_2340_cov_2.992560"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="PLY56_15620"
     CDS             <1..1055
                     /locus_tag="PLY56_15620"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ22955.1"
                     /translation="ALYEAVLKKSPRNQGALARLAAVYEHEKKWPEARKQYARILDLD
                     WTNLDAHLAIAGTYMAEENLDGAIGEFQKVVTKNPKHWDAWKALGRAQERKKDWDGAL
                     QTYARLAELAPKSAYPLASAAMVHLEKGEPAQALESSRKAVARDATLPEAHLALAKSL
                     PEESAAEARKHYLEALRGAVARNNATRREASRRERASGLDPAWVMQAQATLAAGDRVG
                     MEAVAGLRSTSTDEASRKEALEALEALSKEYPASTGLVVAIAGLHEDSGRHADSVPIY
                     RRLVTEHPLDPQLRIQLASAYEKLGKRDEALAQYRRALELDGSNALAAEGIKRLEQPR
                     IEVDPAATGWYKPATK"
     gene            complement(1133..2182)
                     /locus_tag="PLY56_15625"
     CDS             complement(1133..2182)
                     /locus_tag="PLY56_15625"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ22956.1"
                     /translation="MRRLLRASGITAAVLLLSCALAAAAGLSVERKKVNAAGKSFAVT
                     VIRVPREGYRVKVGLANGRVGSTASLASIAQSHGAVAAINGCFFSAYTKDAIKPPWHH
                     VITEGQLVHHGQTGTTLGFDAQGNYRMERLKIRITGTVGADQERPHNWYAYRVNFPPG
                     ASGAILFNRFWAGAKTPAGGKQAVIQNGVVRSVGSGGLPIPRDGYALSFIGGDAHLGN
                     RFQPGDQVTLRIEYGSHDEEFWQSVQEGLGCGPRLLAEGRIAYDPKGEGFNDPKILSL
                     AGARSAIGIRHDGTILLVTCSATVRQLADIMKALGAYDAMNLDGGASSGLWVQGKYLV
                     QPGRSISNALLILKK"
BASE COUNT          406 a          786 c          792 g          356 t
ORIGIN      
        1 gcgctctcta tgaagcggtg ctgaagaaga gccctcgcaa ccagggggca ctggcgcgtc
       61 tggccgcggt ctacgagcac gagaagaagt ggcccgaggc tcggaagcaa tacgcgcgca
      121 tcctggacct cgactggacc aacctcgacg cgcacctggc gatcgctggc acctacatgg
      181 cagaggagaa cctggacggc gccatcggcg agttccagaa ggtcgtcact aagaacccga
      241 agcactggga tgcctggaag gcgcttggac gcgcccagga gcgcaagaag gactgggatg
      301 gtgcccttca gacctatgcc cgcctcgcgg agctggcccc gaagagcgcc tatccccttg
      361 ccagcgcggc gatggtccac ctggagaaag gcgagccggc ccaggccctg gagagcagcc
      421 gcaaagccgt ggcgcgagat gccaccctcc ccgaggcgca cctcgcgctg gcgaagagcc
      481 tgcccgaaga gagtgccgcc gaggcgcgca agcactacct agaggccctg cgcggtgccg
      541 tagcccgcaa taacgcgacc cggcgtgaag ccagccggcg cgagcgcgcg agcggccttg
      601 accccgcgtg ggtcatgcag gctcaagcca ccctggccgc gggcgaccgc gtcgggatgg
      661 aagcggtggc tggcttgcgc agcacctcga ccgacgaggc gtcgcgcaag gaagcgctcg
      721 aggcgctgga ggcgctgtcg aaggagtatc cagccagcac gggcctcgtg gtcgctatcg
      781 ccggcctcca cgaggatagc gggcgccacg ccgattccgt tccgatctac cgccggctcg
      841 ttacggagca cccgctcgac ccgcagctgc gcatccagct ggcgtctgcc tacgagaagc
      901 tcgggaagcg tgacgaggca ctggcgcagt accgccgcgc cctggagctg gatggaagca
      961 atgccctcgc cgcggaaggg atcaagcgcc tggagcagcc gcgtatcgag gtcgatcccg
     1021 cggcaaccgg gtggtacaaa ccggcgacga agtaggccgg gcatgcgctg cgagtagggt
     1081 ctgcccacca cccgcgtggg cagaccagac gtcacgcttc tctccccggc ggtcacttct
     1141 tgaggatgag gagcgcgttg ctgatgctcc ggccgggctg gacgaggtac ttcccctgca
     1201 cccagaggcc gctagacgcg ccgccatcca ggttcatggc gtcgtaggcg ccgagcgcct
     1261 tcatgatatc ggcgagctgc cggactgtgg cggagcaggt gaccaacagg atcgtgccat
     1321 cgtgccggat cccgatggcg ctacgtgccc ccgcgaggct caggatcttg gggtcgttga
     1381 agccctctcc tttcggatcg taggcgatgc ggccctctgc gagcagccgc ggcccgcagc
     1441 caagcccctc ctggacactc tgccagaact cctcgtcgtg cgagccatac tcaatccgca
     1501 gcgtcacctg gtctcccggc tggaagcggt tcccgaggtg cgcatcgccc ccgataaagg
     1561 agagtgcata gccatcacgc gggatcggca ggccgccgga gccaaccgaa cgcacaacgc
     1621 cgttctggat gacggcctgc ttgccgcccg ctggtgtctt ggcgccggcc cagaagcggt
     1681 tgaacaggat ggctccgctg gcgccgggag ggaagttgac gcggtaggca taccagttgt
     1741 gcgggcgctc ctgatctgca cccacggtcc ccgtgatccg gatcttcagg cgctccatgc
     1801 ggtagttgcc ctgggcatcg aagccgagcg tggtgccggt ctgcccgtga tgaacgagct
     1861 ggccctcggt gatcacgtgg tgccagggtg gcttgatcgc gtccttggtg taggcgctga
     1921 agaagcagcc gttgatcgcg gcgaccgcac catgactttg ggcgatcgat gccagcgatg
     1981 ccgtactgcc cacgcgaccg tttgccagac cgaccttgac ccggtagccc tcgcgcggca
     2041 cgcggatcac ggtcacggca aagctcttac ccgccgcgtt caccttcttc cgctccacgg
     2101 aaagcccggc tgcagcggcg agtgcgcagg agagaagcag cacggcggcc gtaatgccgg
     2161 atgcccgaag caatcgtcgc atggcgatgg acccctttcc cgggtggcga gacgccccgc
     2221 cggatgcgcg gcgtcgcccc tcctttcggg tctctgcggc catttcctcc ctggttgctg
     2281 ccagacgctg ggcaaaggtg acgtgggaaa cgttccgttg caggcgcgcg ctagacctca
//
LOCUS       DAOSCV010000737         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29932_length_2338_cov_3.193605, whole genome shotgun sequence.
ACCESSION   DAOSCV010000737 DAOSCV010000000
VERSION     DAOSCV010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29932_length_2338_cov_3.193605"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="rho"
                     /locus_tag="PLY56_15630"
     CDS             complement(<1..946)
                     /gene="rho"
                     /locus_tag="PLY56_15630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ22957.1"
                     /translation="MTAQAETVEGVLEILASGAGFVRTREDLNPSPKDPYVAPSQIRA
                     CGLRTGDRIAGRVRPPSGRERSPSLVVVESIEGEPPDSARERPFFDGLTAISPEERLR
                     LESGPADLTGRAIDLLAPLGKGQRGLIVAPPKAGKTRIIQEIARSVARTTPDAVLLLL
                     LIDERPEEVTDFRREGAGQVYASSFDAESRDHLHLARLVQARAERLVERGRDVVILLD
                     SLTRLARAANLGERGTGRSLSGGLDAAALRMPRQFLGDARKIEGGGSLTILATALVDT
                     GSRMDEVIFEEFKGTGNMEIHLDRKLADRRVFPAIDINR"
     gene            1215..1928
                     /locus_tag="PLY56_15635"
     CDS             1215..1928
                     /locus_tag="PLY56_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HOJ22958.1"
                     /translation="MSEHPETTEHSEHVERSERSPWPWVVLALGLLAFTAAVFFLTRP
                     AQLAPPEGGSTTVIQPGSSPEGPDQRVTVEQDGRRDGEAPTGTEATPPANAPAAPGSP
                     QPAPPRESAPSTAPANQPAQPGGAGQPAPGNRSGVTPSRERQTTTVNVEMGEWFVRLD
                     PATAPPGRVQFRIRNTGSAIHGFEIDGPGVEERSERLDPGEDTVVVANLGPGEYEIYC
                     YVGDHRERGMETRMRVTEE"
     gene            complement(1965..>2338)
                     /locus_tag="PLY56_15640"
     CDS             complement(1965..>2338)
                     /locus_tag="PLY56_15640"
                     /inference="COORDINATES: protein motif:HMM:NF018652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HOJ22959.1"
                     /translation="RNEPYFGFGPSATSYLEGIRSTNARSLSDYLDGIARGERPVEQS
                     EAPSGALAMGETMMLGLRMREGVESARFAARFGIAPEEVYAGPIRRLSDAGLLRAEEG
                     RIALTVRGLLLANNVMAEFLP"
BASE COUNT          387 a          822 c          778 g          351 t
ORIGIN      
        1 agcggttgat gtcgattgcc ggaaatacgc gccggtcggc aagctttctg tcaaggtgga
       61 tctccatgtt gccggtgccc ttgaactcct cgaagatcac ctcgtccatc cggctgcccg
      121 tgtcgacgag ggcggtcgcc aggatggtga ggctgccccc accctcaatc ttccgggcat
      181 cgcccaagaa ctggcggggc atgcgcaggg ccgccgcgtc gagccccccg gagagcgagc
      241 ggcctgtgcc acgctcaccc aggttggccg cacgcgccag gcgagtcagg ctgtcgagaa
      301 ggatcacgac atcgcgaccg cgctccacca gccgctccgc tcgggcttgc accaggcggg
      361 cgaggtggag gtggtcacgc gactcggcat cgaaactgga cgcatagacc tgtccggcgc
      421 cctcgcgacg gaaatcggtc acctcctcgg ggcgctcgtc gatgagcagc agaaggagca
      481 cggcatcggg cgtggtgcgc gccacgctgc gcgcaatctc ctggatgatc cgcgtctttc
      541 cggccttggg cggggcgacg atcagcccgc gctggccctt gcccaggggc gccagcaggt
      601 cgatggcgcg gcctgtcagg tcggctggcc cgctctccag gcgcaggcgc tcttccggcg
      661 agattgcggt cagcccgtca aagaaggggc gctcccgcgc actgtcggga ggctcgccct
      721 caatgctctc caccactacc agcgagggag aacgctctcg gcccgacggc gggcgcaccc
      781 gcccggcgat gcggtcgccc gtgcgcaggc cgcaggcccg gatctgggaa ggagccacgt
      841 agggatcttt cggcgaggga ttgaggtctt ctctcgtgcg cacgaacccc gctccactcg
      901 ccaagatctc gagcacgccc tccaccgttt ccgcttgtgc agtcatctta ccacctattc
      961 tcatcgagga ggcgatggga cggcctggtt gatgccgccc gcgcgaggag cagaatattg
     1021 tggccattct ctgcccactc tcttccccct cgcccctatt gtaacacatt tgatgagacc
     1081 gctcccacac catgcccttg ctctccgggt ggcatcgacg gtgcaatcct gttttgcgcc
     1141 agacactccg gggaatgatg ccggcaacac tcctgcggct tatcaagcac gctcagggca
     1201 tggaggagcc tggcatgtca gaacatccag agaccacaga gcactcggag cacgtcgagc
     1261 gcagcgagag atccccctgg ccgtgggtcg ttctcgccct cggcctgctg gcgttcaccg
     1321 cggccgtctt cttcctaacc cggccggcgc agctggcgcc ccccgaagga ggcagcacaa
     1381 cggtcatcca gccgggctcg agcccggaag ggccagacca gcgggtgacg gttgagcagg
     1441 atggccgccg ggatggagag gcgcccaccg gcacggaggc gacgccgcca gcgaatgccc
     1501 ctgccgcgcc aggaagcccg cagccggcac cgccgcgcga gtcggcgccc tcgacggcgc
     1561 ccgccaacca gccagctcaa ccgggtggcg ccgggcagcc ggccccaggc aaccgttcag
     1621 gcgtaacccc gtcccgcgag cggcaaacga ccacggtgaa cgtcgagatg ggagagtggt
     1681 tcgtgcgcct ggaccccgcg acggcaccgc ccgggcgcgt ccagtttcgg atccgcaaca
     1741 ccggctccgc gatccacggc tttgagatcg acgggccggg cgtcgaggag cggagcgaac
     1801 ggttggatcc tggtgaggac acggtcgtgg tcgccaatct gggcccggga gagtacgaga
     1861 tctactgcta cgtcggcgat caccgcgagc gcgggatgga gacgcggatg cgggtcacgg
     1921 aagagtgatc gccactcggt ggccagcgag cggcatctcg ccgcttaggg caggaactcc
     1981 gccatcacgt tgttggcgag gaggagcccg cgaacggtga gggcgatccg gccctcctcg
     2041 gcgcgcagca agccggcgtc cgagaggcgg cggatcggcc cggcatacac ctcctccggc
     2101 gcgatgccga accgcgccgc gaagcgcgcg ctctcgacgc cctcgcgcat ccgcaggccc
     2161 agcatcattg tctcacccat cgccagcgcg cccgatggcg cttccgactg ctccacgggc
     2221 cgctcgccgc gcgcgatgcc atcgagataa tccgatagcg agcgggcgtt ggtgctccga
     2281 atcccctcca gatagctcgt ggccgacggc ccgaagccaa aatagggctc gtttcgcc
//
LOCUS       DAOSCV010000738         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_29969_length_2336_cov_2.609820, whole genome shotgun sequence.
ACCESSION   DAOSCV010000738 DAOSCV010000000
VERSION     DAOSCV010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29969_length_2336_cov_2.609820"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PLY56_15645"
     CDS             complement(<1..1135)
                     /locus_tag="PLY56_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22960.1"
                     /translation="MDASDRLVDILQQLGACLSSREETPHAWRPLGEALLAVPGVSGV
                     WFALWREADLEALSLGELPGEEQARDDLLRGWRWDYRAEGAPVASVLATPGPLVIAPL
                     GEPDHLAGALALATHEPVAPLMRLAEVVASGLRADLRRRRLAGELEQARTLLYEQMSG
                     ISELPAVSQLAASVAHELRNPLSSIKGAAQYIRNEYQDHATVREFLDIIIEEVGVLNR
                     ITTEFLDFARPMHLNLEQMHVHDSIRRLLQVMKPQMAAQGIEAVLDLSADVPPTYFDS
                     RQVDQMLRNIVMNAIQAMPDGGTLRVRTRTFDTPLPGVEIAIADTGRGINPEDLHRLF
                     TPFFTTKTKGVGLGLTIVQRIAQNHGGVVTVQCPPGGGSEFTIR"
     gene            complement(1486..>2336)
                     /locus_tag="PLY56_15650"
     CDS             complement(1486..>2336)
                     /locus_tag="PLY56_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22961.1"
                     /translation="EHLANYYAQTVAHNCVLVHQPGEPPARYWGGTVIANHGGQHRQL
                     GSEVKAFETNDRYVYVAGDATASYLHGPVKREGQPDLPEKVSLVTRQLVFLMPKHFVV
                     FDRVVATDPSYRKEWLLHTAHEPVIAGKQFRADHEEGRLFCRTLLPEDAVLTPVGGSG
                     REFWAAGRNWEIKADKLKPEQLAMMGQWRVEVSPGAARAEDHFLHVLQVGDQQLSAMD
                     PVEVIREGDAVGARVRVGETTWEVLFNTKGDLGGRIRCRGGNATIDQALAQTVTPQVG
                     ILALPK"
BASE COUNT          403 a          799 c          765 g          369 t
ORIGIN      
        1 gacggatggt aaactcgctc ccgcccccag gagggcattg caccgtgacc accccgccgt
       61 ggttctgcgc gattctctgg acaatggtca ggccgagacc cacgcccttc gtcttggtgg
      121 tgaagaacgg ggtgaagagc cgatggagat cctcggggtt gatgccccgg cctgtatcgg
      181 cgatcgcaat ctccaccccg ggcagcggcg tgtcgaaggt acgcgtccgc actcggagcg
      241 tacctccgtc cggcatcgcc tgaatcgcgt tcatgacgat gttgcgcagc atctgatcca
      301 cctggcgcga gtcaaagtag gtcggaggca cgtccgcgct cagatccagc accgcctcga
      361 ttccctgcgc ggccatctgc ggcttcatca cctgcaggag acggcgaata ctgtcgtgca
      421 cgtgcatctg ctccaggttg aggtgcatcg gccgggcgaa atcgaggaac tccgtggtga
      481 tccggttgag gacgcccacc tcctcgataa taatatcgag gaactcgcgc acggtggcat
      541 gatcctggta ttcgttgcgg atgtattgcg cggcgccctt gatagagctc agcggattac
      601 gcagctcgtg cgccacgctg gcggccagct ggctgacagc cggtagctcg ctgatcccgg
      661 acatctgctc gtacaggagc gtccgggctt gctccagctc acccgccagt cgccgccggc
      721 gcagatcggc ccgcaagccg ctggccacca cttcggcgag gcgcatcagg ggcgccaccg
      781 gttcatgcgt tgccagagca agggcgcctg ccagatggtc cggctcgccg agcggcgcga
      841 tcacgagtgg ccccggagta gcgagaacgc tggccacggg agcaccctca gcccggtagt
      901 cccagcgcca cccgcgcaga agatcgtcac gcgcctgctc ctcgcccggt agctccccga
      961 gcgacagcgc ctccagatcc gcctcgcgcc agagcgcgaa ccacactccc gagacgcccg
     1021 gcacggcgag cagcgcctct cccaaggggc gccaggcgtg cggcgtctcc tcccgcgatg
     1081 aaagacatgc cccgagttgc tgcagaatat ctaccaagcg atcactcgcg tccattcatc
     1141 accctattgc ccggaccaac cagcagcgca gccgccgcgt gccccactcc acatgacggg
     1201 aatcagttac ccccggaacc acggggccaa acctgcctgc gggggcgtag aacaggcggc
     1261 aggaggacac acggggaggc ccacgagatg accgtctcat cggccaggcg gctcttggaa
     1321 gcaagctcct cggctaccac gccggggcgc ggtcgaagct ggaagagggg cgcgggcaat
     1381 gggacaacaa agcgtgcggc aatccacccc cgcgggtgaa cttgcgcggg aagggcggtg
     1441 ggcacccttc ccgcgcgagc ggattggagt tcgagtgagc gtgccctact tggggagggc
     1501 caggatgccg acttgagggg tgaccgtctg agcgagcgcc tggtcgatgg tcgcgttgcc
     1561 gccccggcag cggatacggc cacccagatc gcctttcgta ttgaagagca cttcccaggt
     1621 ggtctcgccc acccgaacgc gcgcgccaac cgcgtcgccc tcgcggatca cctccacggg
     1681 atccatggca gagagctgct ggtcgcccac ctgaaggacg tggaggaagt ggtcctcggc
     1741 gcgagcggcg cccggtgaga cctccacccg ccactggccc atcatcgcca gctgctcggg
     1801 cttcagctta tccgccttga tctcccagtt gcgcccggcc gcccagaact cgcggcccga
     1861 accgcccaca ggagtgagca ccgcatcctc cggcaggagc gtacggcaga agagccggcc
     1921 ctcctcatgg tcggcgcgaa actgcttccc cgcgatgacc ggctcgtgcg ccgtgtgcag
     1981 cagccactct ttccggtagg aggggtccgt ggcgaccacg cggtcgaaaa ctacgaagtg
     2041 cttcggcatc aggaagacca gctgccgggt gacgagcgag accttctccg ggaggtccgg
     2101 ctggccctcg cgcttcaccg ggccatggag gtagctggcg gtggcatcgc cagccacgta
     2161 gacgtagcgg tcgttggtct cgaaagcctt cacctcggag ccgagctggc ggtgctggcc
     2221 gccgtgattc gcgatcaccg tgccgcccca gtagcgcgcg ggtggctcac ctggctggtg
     2281 gacgaggacg cagttgtgcg ccacggtctg cgcgtagtag ttggccaggt gctcgc
//
LOCUS       DAOSCV010000739         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30041_length_2331_cov_3.871265, whole genome shotgun sequence.
ACCESSION   DAOSCV010000739 DAOSCV010000000
VERSION     DAOSCV010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30041_length_2331_cov_3.871265"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..782)
                     /locus_tag="PLY56_15655"
     CDS             complement(36..782)
                     /locus_tag="PLY56_15655"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ22962.1"
                     /translation="MFVHRLLSALVGIPVGIFLVWQGGLWLAGFVVILGVIAFQEYHA
                     TLRIRRVEALREIGFPASAALILGAYFFGGPELLLFTQGVLALAVVSSLAFHLFAPIE
                     GSRTMSAAATLLGVVYIGFLFTFTVLVREMVGPTRTGLPFGLHLFLLAWLATMAADTV
                     AYAVGKALGKRKLCPAVSPGKTVEGAIGGVLGSMAFALPLGIWAGFPAGHAIALGALI
                     AVAVESAEEESCPSQYPSGFNRASRCAAAA"
     gene            1474..2166
                     /locus_tag="PLY56_15660"
     CDS             1474..2166
                     /locus_tag="PLY56_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22963.1"
                     /translation="MKKRILVVDDEEQIQRILRRNLVTRGYEVVVASDGEDALDQAAE
                     KEPDVVVLDLVMPRIDGLEFTRRFREWSKAPIIVLSAIGEERAKVQALDLGADDYVTK
                     PFGLEELLARIRSALRRSALAACEAHSGPTFQAGDLHIDFHRRVVTLCGEDVRLTPTE
                     YELLKFMVTNANRVLTHNMLLKAVWGNRHGAEPEYLRVFVSQLRRKLRDDSSSPRYIL
                     TEPSVGYRFRAE"
BASE COUNT          426 a          764 c          763 g          378 t
ORIGIN      
        1 gccacggcct cccactcgcg gatcgccgcg gcccatcacg cggcggcggc gcacctgctg
       61 gcgcggttga aaccagaggg atattgggag gggcagcttt cctcctcggc gctctcgacg
      121 gcgacggcga tgagcgcgcc gagggcgatg gcgtggccgg cgggaaagcc cgcccagatc
      181 ccgagcggca gcgcaaacgc catcgatccc agaacgccgc cgatagcgcc ctccaccgtc
      241 ttccccgggc tcaccgccgg gcagagcttg cgcttgccga gcgccttgcc aaccgcgtac
      301 gcgaccgtat cagccgccat cgtggccagc cacgccagca ggaagaggtg cagcccgaag
      361 gggaggccgg ttcgcgtcgg gccgaccatc tcgcggacga ggaccgtgaa ggtgaagagg
      421 aagccgatgt agaccacgcc gagcagagtc gctgcggcgc tcatcgtgcg cgagccttct
      481 atcggagcga agagatggaa cgccaggctg ctgactaccg cgagcgccag caccccttgc
      541 gtaaagagga gcagctcggg gccgccgaag aagtaggcgc ccagaatgag cgccgcggac
      601 gcggggaatc caatctcgcg gagcgcttcg acccgccgga tgcgcagagt ggcgtggtac
      661 tcctggaagg cgatcacgcc caggatcacc acgaagccgg caagccagag ccctccctgc
      721 cagaccagga agatgcccac gggaatgccc acgagcgcgc ttaacaggcg gtgtacaaac
      781 acgggcgtcc tctctgcact tggggttcca atgataagaa ggacacgaat ggggagagcc
      841 atctacacgc gcggagcttc agctccgcgc tgcttcccgg caaagccctt cgggcttccc
      901 ctcccagccc ctggcgttct agggcatcgg gaagagtagc caaacccagc ccggcagggc
      961 ttcgctcaca agcagcgcgg agattaatca ccgcggactg ggttgcgcct caccttcgaa
     1021 gcttcgcact ccttatctcg gaatcttagc tctgcccaac ggtccttgcg aacaccctgt
     1081 ggtagcagtt cgccgcggcg gaggagcatc ctgcccccgg ccggagaacc tcgcgcgcgg
     1141 gccgcccgcc ggccaatccc cggctgcgga acgcgggttc tcggagggat ccccgagttg
     1201 cgcgggtggt tcgattccga gggattgcat aaccgaaggc gccgcacgtc cccatccgtg
     1261 tccggccccg tcatccgtgg tatcgccccc ggcgcggggc cgggggtagg gaagccgcag
     1321 catcgagcgc ctatttgaca ctctcctggc gatatgctaa gattccggat ggccctgata
     1381 ccccgagatg gcggagcctt ccatcggccc gggatgtgtg gtttcgcccg gctgggagtg
     1441 tcgccagagg cgctctgtga tgaggtccgg tctatgaaga agcgcatcct ggtagtagat
     1501 gacgaggagc agatacagcg tatcctccgc cggaaccttg ttactcgcgg gtatgaggtg
     1561 gtggtggcct ccgatggcga ggatgcgctg gaccaggcgg cggagaaaga gccggatgtc
     1621 gtggtactcg atctggtcat gcctcgcata gatggcctgg agttcacccg gcgcttccgc
     1681 gagtggtcca aagccccgat catcgtgctt tccgctattg gcgaggagcg ggccaaggtc
     1741 caggcgctcg atctgggagc ggatgattac gtcacgaagc cctttggcct ggaagagctg
     1801 ctggcgcgaa ttcgctccgc gctccggcgc tccgctctcg ctgcctgcga ggcccatagt
     1861 ggccccacgt tccaagcggg cgacctgcac atcgatttcc atcggcgggt tgtcacgctt
     1921 tgcggcgaag acgttcggct cacccccact gagtacgagc tgctgaagtt catggtcacc
     1981 aacgccaacc gtgtcttgac acacaacatg ctgctgaagg cggtatgggg caaccggcat
     2041 ggggcagaac cagaatatct gcgcgtcttc gtgagccagc ttcgccggaa acttcgcgat
     2101 gattcctctt cgccccgcta cattctcaca gagcccagcg tcggctatcg ctttcgggct
     2161 gagtaggcct ctcaggacgc gtgggaccgc tcgcgctgct cccaagcgcg ccccggcagg
     2221 agaccggtgc caccgctggg attccgacat aaggaatccg aagcctggga ggcagggcgc
     2281 aacccagcgc gcggcgataa atcaccgcgc tgcttgcgag cgaagccctg c
//
LOCUS       DAOSCV010000740         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30042_length_2331_cov_3.488137, whole genome shotgun sequence.
ACCESSION   DAOSCV010000740 DAOSCV010000000
VERSION     DAOSCV010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30042_length_2331_cov_3.488137"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1509)
                     /locus_tag="PLY56_15665"
     CDS             complement(196..1509)
                     /locus_tag="PLY56_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ22964.1"
                     /translation="MLGGIGLEVTVLLLLILANGLFAAAEMALVSARRAILEHQAEEG
                     DAGAAAALALVDAPNDFLATVQVGITFISTFAGAFGGASVVRALAPLLADLRVPFITR
                     YAESIALGVVVLMITYLSLVIGELVPKRLALRQSERLARRLARPMRWLSGLARPIVQI
                     LGTSTDAVLRVLGSREPQHEETATREIGYLLETGMRHGEIEPAEAELVEEVFRLAETR
                     ARDAMTPRPDIVALPETASIHEAQRLALSSGYSRIPVYHHSIDEIIGFIHVRDLLDAC
                     MQGEPTELPEILRKPLFVPETAPISALLPRLREERAHLAVVLDEFGSTAGIITLEDVL
                     EQLVGEIREEHRPEEAGIVQRSETSWLVDGTVSLEDLRNTLTVTEWPDRERLGYTTLA
                     GFILARLGRLPRRGDAVDWQGYRFEVVDMDLRRIDQVLITRIDEE"
     gene            complement(1753..>2331)
                     /locus_tag="PLY56_15670"
     CDS             complement(1753..>2331)
                     /locus_tag="PLY56_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOJ22965.1"
                     /translation="VDLLSLSAHKFYGPKGMGVLYARKGTRRQPLFRGGGQERGHRPG
                     TENVPGIVGTATALDQAVGLLSTEAERIRALRDRLIAGIEFSVPGCRLNGHPLQRLPN
                     NAHFSVEGVESEALLVSLDMHGIAASGGSACMSGALEPSHVLQAIGVPMEAARGSVRF
                     SLGRGNTAEEIDRVITLFAETVERLRGLSRSA"
BASE COUNT          392 a          774 c          785 g          380 t
ORIGIN      
        1 agcccggcag ggcttcgctc gcaagcagcg tggtgattta tcgccgcgcg tcgggttgcg
       61 cgctgcctcc gtggctccgg tttccttatg tcgaaatctc cgagcccgca gcccgtcgcg
      121 cccccatcac gggcctggat gggccgctca agagggatgc ctggccggcc ccactgactg
      181 acggttctgc gcggctcact cttcatcgat gcgcgtgatg agcacctggt cgattcgcct
      241 taagtccatg tcgaccacct cgaagcggta gccctgccaa tcgactgcat cgccacggcg
      301 gggcaggcgc ccgaggcgcg ccaggatgaa gccggcgagc gtcgtgtagc ccaggcgctc
      361 gcgatccggc cattcggtca cggtgagcgt gttgcggagg tcctccaggc tcaccgtacc
      421 atccaccagc catgaggttt cggagcgctg gacaatgccc gcctcctcgg ggcggtgttc
      481 ttcgcggatc tcacccacga gctgctcaag gacatcctcc agggtgatga tcccggcggt
      541 gctcccgaac tcgtcgagca ccacggcgag atgggcgcgt tcctcgcgaa gccgggggag
      601 gagcgccgag attggagccg tctcgggcac gaagagcggc ttgcggagga tctctggcag
      661 ctccgtcggc tctccttgca tgcaagcatc cagcaaatcc cgcacgtgga tgaacccgat
      721 gatctcatcg atagagtggt gatagacggg gatgcgggag tagccggagg agagggcgag
      781 gcgctgcgct tcgtggatgc tggccgtttc gggcagcgcc acgatatcgg gccgtggggt
      841 catggcgtca cgggcgcgtg tttcggccag gcggaagacc tcttccacca gctcggcctc
      901 tgccggctcg atctcgccat ggcgcatccc cgtttccagg aggtagccga tctcgcgagt
      961 ggccgtctcc tcgtgttggg gctcgcgact ccccagaacg cggagcacgg catcggtaga
     1021 ggtcccaagg atctgcacga tgggacgcgc caagccggag agccagcgca tcggtcgtgc
     1081 caggcgccgc gcgaggcgtt ccgactggcg caatgccagg cgcttgggca ccagctcgcc
     1141 gatcaccagc gagaggtagg tgatcatcag caccaccacg cccagagcga tgctctccgc
     1201 gtagcgcgtg atgaagggca cccgcagatc tgccagcaac ggcgcgagcg cgcggactac
     1261 gctggcgcca ccgaacgcac cggcgaaggt gctgatgaac gtgatcccca cctgaaccgt
     1321 cgccagaaaa tcgttcggcg catctaccag cgccaacgcc gctgctgcgc ccgcgtctcc
     1381 ctcctctgcc tggtgctcca gaatcgcccg ccgcgccgag accagcgcca tctcggccgc
     1441 ggcaaagagt ccattggcca gaatgaggag gagaagaacc gtgacctcaa gaccaatccc
     1501 gcccagcacc gcatctcctc ggaggcgcga cctcgaggga gtttttcccc gggtgctggc
     1561 atcctacact cccacgatgg cccgaggggt gcatatgaag cacggcgaga gagaggctgc
     1621 gtcttccctg cccagcgagg agcgtgcgag ggggatcaga ttctctacct ggctgttttg
     1681 tggcgccggc cgccggcccg gcggtgaacg gctccgagag cggctgtgat tccgcccatg
     1741 aggggaaggg gctcacgcgg agcgggagag accccggagg cgttccaccg tctcggcaaa
     1801 gagggtgatg acgcggtcta tctcttcggc ggtgttccct cggcccaggc tgaagcgcac
     1861 cgacccgcgg gcggcctcca tcggcacgcc gatcgcttga agcacatggg atggctcgag
     1921 ggcgccggac atacatgccg atccaccgga ggccgcgatt ccgtgcatgt ccaggctgac
     1981 gaggagggct tcgctctcga cgccctctac actgaagtgg gcgttgttgg ggagccgctg
     2041 gagcggatgt ccattcagcc ggcagccagg cacggagaac tcgatcccgg cgatgagccg
     2101 gtcgcggagc gctcggatcc gctccgcctc cgtggagagc aggccgaccg cctggtcgag
     2161 cgccgtggcc gtgccgacga tgccaggcac gttctcggtt ccagggcgat gtccgcgctc
     2221 ttgcccgccc ccgcgaaaga ggggctgcct gcgcgtcccc tttcgggcgt acagcacgcc
     2281 catccccttg ggcccataga acttatgcgc agaaagggag aggagatcga c
//
LOCUS       DAOSCV010000741         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30130_length_2327_cov_2.734155, whole genome shotgun sequence.
ACCESSION   DAOSCV010000741 DAOSCV010000000
VERSION     DAOSCV010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30130_length_2327_cov_2.734155"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PLY56_15675"
     CDS             complement(<1..1205)
                     /locus_tag="PLY56_15675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ22966.1"
                     /translation="MGRTGDGDAPIRHMTARSSRAIKTGYQRFERPVYDYAAKYAPCR
                     RGCPAGHDTAWATCMISKGRYDLALRVLREENPFPSVTGRVCHHPCEDACSRGDYDQP
                     VAIQALERACAEYGDTDPVELAPRLHEESVGIIGAGPAGLACAYHLVRLGYPVTVYDA
                     NDTPGGILAYGIPAYRLPREVLETEIDRLRRMGVAFRCGVRIGVDLSFAQLRQSHHAL
                     FLAPGLARARQLSVPMPKDPRVESGVAFLRAARLGVARSVSGHVVVIGGGDVGIDAAR
                     SALRLGAEKVTLCCGEARTEMPAHPEEIEAARHEGVEILDRVVPARVDATRNSVNLTL
                     LPVRAMRRDPDGTPRFDVEEGPGWQIRAARLLYAVGQEADLSFLPPSLAQGQRLRVDA
                     WGQTSLPGV"
     gene            complement(1524..>2327)
                     /locus_tag="PLY56_15680"
     CDS             complement(1524..>2327)
                     /locus_tag="PLY56_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22967.1"
                     /translation="RNSQALFDRAEAAVAGDPVLLRRVRHARLPLDRASLVLLPRLMR
                     EWVARGNNPEVLPLDRDAVAQRCKATWYAEVDRRIPEKRRQAERAKADEEILPLITRR
                     PYVPLPEKFRNLPAGTVFDYTAETSRNWQNRVKVVPDPEAESGITNRLELTDEEMEKY
                     ALPMPWGLYNQIAKKGVGSVSIRPEDVPGPGYHWYRMGSFPIAHSTYVYFFWSWIIQV
                     DVDDVADSKRPDQKYTIWARVKFEGPRFPHGKPDQKNAICVERVVLVKE"
BASE COUNT          374 a          773 c          806 g          374 t
ORIGIN      
        1 aagacgccgg gcaggctggt ctggccccag gcatcgacgc gcaaccgctg gccttgcgcc
       61 agcgacggcg ggagaaagga gaggtcggcc tcctgtccga cggcatagag gaggcgcgcc
      121 gcgcggatct gccatccagg gccttcctcc acatcgaacc gcggggtgcc gtccggatcc
      181 ctgcgcatcg cgcgcacggg gaggagggtc agattcacgc tgttcctggt ggcatccacg
      241 cgggcgggca ccacccggtc aagtatctcg acgccttcgt gccgggccgc ctcgatctcc
      301 tcgggatggg caggcatctc ggtgcgtgcc tcgccacagc agagggtgac tttctcggcg
      361 cccagacgca acgcggagcg ggcggcatcg atacccacgt cgcccccgcc gatcaccacc
      421 acgtggccgc tcacggagcg cgctacccca agcctggccg cgcgcagaaa cgccaccccc
      481 gattcaacgc gcgggtcctt tggcatcggc acggagagct ggcgtgcgcg tgccaatcct
      541 ggcgccagaa agagggcgtg atgcgactgg cgcagctgcg cgaaggagag atccacgccg
      601 atgcgcacgc cgcagcggaa cgccacgccc attcggcgca gccggtcgat ctcggtctcg
      661 agtacctcgc ggggcaggcg gtaggcggga atcccgtagg ccaggatccc gccgggagta
      721 tcgttggcat cgtagacggt gaccggatag ccgaggcgaa cgaggtgata ggcacacgcc
      781 aggcccgcag gcccggcgcc gatgatgccg acgctctcct cgtgaagccg cggcgctagc
      841 tcgacgggat cggtgtcgcc atactcggcg caggcgcgct cgagggcctg gatcgcgacc
      901 ggctggtcgt aatcgccacg gctgcaggca tcctcgcagg gatgatggca gacgcgcccg
      961 gtgaccgatg ggaaggggtt ctcctcgcga agcacgcgca gggccaggtc gtagcgcccc
     1021 ttgctgatca tgcaggtggc ccaggccgta tcgtggccgg cggggcaccc tcggcggcag
     1081 ggggcatact tggcagcgta atcatacacg gggcgctcga aacgctggta tcctgtcttg
     1141 atcgcgcgac tgctccgtgc cgtcatgtga cggatcggag catcaccgtc gccggtcctc
     1201 cccataccaa ctctccatcc agccacgcgc cgccatgagg tgtggcaatc gaccggcgcg
     1261 tgagagggtg cgctccggca ggcttgcggg acgggccatc aaggcagaga ttgcgtcgcc
     1321 gcttccgggc agggtgccag aacgcctctg cactgttaga gggtgcttgc caggccgttt
     1381 gtttcaccag ggggatgccg agttgtggat gccggacggg ggcagcgcga cgcgtcgggc
     1441 gcccgctgga tgcgcaggga tgcccgccag gccctcccct gccaggggag ggcctggcag
     1501 atggttggtc ggatgctcgg agactactcc ttcaccagca ccacgcgctc gacgcagatt
     1561 gcgttcttct ggtccggctt tccatgcggg aagcggggcc cctcgaactt cacgcgcgcc
     1621 cagatggtat acttctggtc agggcgcttg ctgtcggcca cgtcatcgac atccacctgg
     1681 atgatccagc tccagaagaa gtagacatag gtggagtggg caatcggaaa gctgcccatg
     1741 cggtaccagt gatacccggg tccgggcacg tcttccggcc ggatggagac gctcccaacg
     1801 ccctttttcg cgatctggtt gtagaggccc cagggcatcg gcagcgcgta cttctccatc
     1861 tcctcgtccg tgagctccag gcggttggtg atcccgctct cggcctcggg atcgggcacc
     1921 accttcactc ggttctgcca gttgcggctc gtctcggcgg tgtagtcgaa gacagtgcca
     1981 gccggcaggt tgcggaactt ctcgggcagg ggcacgtagg gccggcgtgt gatgagaggg
     2041 agtatctcct catcagcctt cgcgcgttcg gcttgacggc gcttctcggg gatgcgccgg
     2101 tccacctccg cataccacgt cgccttgcat cgctgggcaa ccgcgtcgcg atccaagggc
     2161 aacacctcgg ggttgttccc gcgggccacc cactcgcgca tgaggcgagg caagagcact
     2221 agcgatgccc ggtcgagagg cagccgcgcg tgccgcaccc ggcgcagcag gacaggatcg
     2281 cccgcgacgg cggcctcggc ccggtcgaac agcgcctggg agttgcg
//
LOCUS       DAOSCV010000742         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30202_length_2323_cov_3.302469, whole genome shotgun sequence.
ACCESSION   DAOSCV010000742 DAOSCV010000000
VERSION     DAOSCV010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30202_length_2323_cov_3.302469"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLY56_15685"
     CDS             complement(<1..418)
                     /locus_tag="PLY56_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22968.1"
                     /translation="MATLPLVRATTSDVGSQRAPLPSATEWAQMLHYAETLRALNDRL
                     RTSYEQLSSLLILGQAFNAAGSRGRVGEILMEIAREVLPYRAAAILLYEEGTYHRRGT
                     VDLTPELDAELSRQMMDGVLEWVREAGQPTTRAADGD"
     gene            complement(418..1293)
                     /locus_tag="PLY56_15690"
     CDS             complement(418..1293)
                     /locus_tag="PLY56_15690"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOJ22969.1"
                     /translation="MLADKVKQHVMELRDLPSLPEVVARVLQVVDNPRSSAADFGRAI
                     SLDPALTARILRLANSAFYGYTGRIGTAVQAVTILGFAQVRSLVLTVSILDLFWHSGK
                     RPALPHYDFWRHCIACAVAARALARRTRICPPEEAFVAGLLHDLGKMVLATVIPDEYH
                     QVVKQAEAEGRVLVEVEQECLSLTHSMVGGWAAEKWRLPMALNQAVERHHEWVADAPA
                     AGLARCVFFANRVVGSGEFGWSGNGQPLEIDTRLLAGTGIDPADLDAIPREIAEDYQS
                     ACQLLDVIGSKGEGF"
     gene            1564..>2323
                     /locus_tag="PLY56_15695"
     CDS             1564..>2323
                     /locus_tag="PLY56_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22970.1"
                     /translation="MRSPADSGNRGLRRGLFHLATALVLNACVLSTGVLGEEPPARVE
                     VTLLPEVTVRGPQIRLGDLGVLRGDPTLAARAREVIVGQAPLPGRERVVELSYIHIRM
                     RQSRLNPEHFDFLAPDRIVVRRAGRTVSPEEQIRAAEAAVRRVCNPRDGEKVIVEPLS
                     TPLPLTVPEGELQLIADPGEGNAHASSVVAVRLRALVDGTLAATASVTVRVRRLSPVW
                     VATRALSYGTLLAEGDARVEQRDVAGIAQPLAPNA"
BASE COUNT          403 a          799 c          733 g          388 t
ORIGIN      
        1 ggtcgccatc cgcggcgcgc gtcgtgggct ggccagcttc acgcacccat tcgaggacgc
       61 catccatcat ctgccgcgat agctcggcgt ccaactccgg cgtcaaatcg acggtgcccc
      121 gccggtgata cgtcccttcc tcatagagga gaatggcggc agcgcggtag ggaagcactt
      181 ctctggcgat ctccattagg atctcgccga cgcgcccgcg actgccagcc gcgttgaacg
      241 cctgccccag gatcaagagc gacgagagct gctcgtagga agtacgcagc cggtcgttca
      301 gcgcgcgaag cgtttcggca tagtggagca tctgcgccca ctccgtcgcg ctcggcagcg
      361 gcgccctctg gctccccaca tcggatgtcg ttgcacgaac gagcggaagc gttgccatca
      421 gaaaccctcc cccttggatc caatcacatc cagaagctgg cacgcgctct ggtaatcctc
      481 ggcgatctcc cttggaatcg catccaggtc ggccggatcg atcccggttc ccgccaggag
      541 gcgggtatcg atctcgaggg gctgtccatt cccggaccat ccgaactccc cgctccccac
      601 tacccggttg gcaaagaaga cgcaccgcgc caggccggcg gcaggcgcat ccgccaccca
      661 ttcgtggtgc cgctcgacgg cctggttcag cgccatgggc agccgccact tctcggccgc
      721 ccaaccaccc accatggagt gcgtgaggct caggcactcc tgctccacct ctaccagcac
      781 ccttccctcg gcctccgcct gcttcaccac ctggtggtac tcgtcgggaa tgaccgtggc
      841 cagcaccatc ttgcccaggt cgtgcaacag gccggcgaca aaggcctctt ccgggggaca
      901 gatgcgggtt cgacgcgcca gggcgcgcgc ggcgacggcg cacgcgatgc agtgccgcca
      961 gaaatcgtaa tgcgggaggg ccggcctctt cccagaatgc cagaagagat ccagaatgct
     1021 gacagtgagg accagactgc gcacctgagc aaagccgagg atggtcaccg cttgaacggc
     1081 cgtgccgatc cggcccgtgt agccatagaa tgctgagttg gccaggcgca ggatgcgcgc
     1141 ggtcagcgcg ggatccagcg agatggcccg cccaaaatca gccgccgatg agcggggatt
     1201 gtccacgacc tggagcacgc gtgccactac ctctggaagg ctcggcaaat ctcggagctc
     1261 catcacgtgt tgcttgacct tatcggccag cattcgtcct attcctctcg agagccccgg
     1321 agcgctcccg gcggggctcc cctaacgcgt gtcaagaaac gcgccatcta actgcattat
     1381 gggaggtctg actcctcaac gatttcttcg gccgaaccgc tacccgcctt aagtttgaga
     1441 agagggaact gcctccgggt ttaagattgt ctcctcgaac tgccgaagca gagttgaatc
     1501 gttttccggg ccaggacgga ccagcggaac acctggccgt atcgcgagag ggagctgatg
     1561 gggatgagaa gcccagccga tagcgggaac cgcgggctgc gccgtggcct cttccacctc
     1621 gccaccgcgc tcgtgctgaa tgcgtgcgtt ctctccaccg gggttttggg tgaagagccg
     1681 cccgctcgcg tggaggtgac gctgctgccc gaggtgacgg tgcgcggacc acagatccgt
     1741 ctcggcgatc tgggcgtgct ccggggcgac ccgacgctcg ccgcccgggc gcgtgaggta
     1801 attgtcggcc aggcgccgct ccctgggcgg gaacgagtgg tggagctcag ctatatccac
     1861 atccgcatgc gccagagccg gctcaacccc gaacacttcg acttcctcgc gcccgaccgg
     1921 attgtagtcc gcagagcagg acgcaccgtt tcacccgagg agcagattcg cgccgccgaa
     1981 gcggcggtgc gccgcgtctg caacccgcgc gacggggaga aggtgatcgt cgagccactc
     2041 tcgactcccc tccccctgac tgtgcccgag ggggaacttc agctgatagc ggatcccggt
     2101 gaggggaacg cccatgcctc gagcgtcgtg gccgtccggc tgcgcgctct ggtggatggc
     2161 accctcgctg cgacggcaag cgtcaccgtg cgagtgcgcc gcctctcgcc ggtctgggtc
     2221 gcgacgcggg ctctcagcta tggcaccctc ctcgcggagg gtgacgcacg cgtcgagcag
     2281 cgcgatgtcg ccggaatagc ccagccgctt gcgcccaatg cgg
//
LOCUS       DAOSCV010000743         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30287_length_2319_cov_2.837898, whole genome shotgun sequence.
ACCESSION   DAOSCV010000743 DAOSCV010000000
VERSION     DAOSCV010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30287_length_2319_cov_2.837898"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1293
                     /locus_tag="PLY56_15700"
     CDS             238..1293
                     /locus_tag="PLY56_15700"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HOJ22971.1"
                     /translation="MEARLKPAVARILDELRSRPKGIKKMSARRLLRAFGYERRSQEN
                     VREIRAELARARIEVNLAVDDPKTLDELVTLRLRKATRALPVDASDAPPAGGKTVGDR
                     PLSPLPTPAQIAQGRHRDITDVIAAVKQCTVQIFTEIGEGSGVIVDPNGLILTNRHVV
                     DTEEGLSERYVHVHFADGTEDDATVVRSHRPLDYALLVVPRQGLCAVPLGDPLKLKDA
                     KTLYAIGNPGGLAFSVTRGIVSAARRVCTTNVEYIQTDAAINAGSSGGPVVTEQGDLV
                     GISAWAYRGDGRLQAQGINFAIPVDYMREEIRALRAIGFQEACRRYYCRHCGWLEESE
                     GRYCSNCGAARTEGSER"
     gene            1290..1838
                     /locus_tag="PLY56_15705"
     CDS             1290..1838
                     /locus_tag="PLY56_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22972.1"
                     /translation="MRPPAAHHRLGVSLLQQRERGVPQLAEHHARTPEQRETLRAQGA
                     QAIMDGMKRLGASDPTALQDENGFLHFPVGSIQCTLGIVTLEERDLFLVYVPIMDLPS
                     DRDLILPLYRLLLELNNRFELSIAKFGLHGDTIFLSAMRPLDSLDPTEVEDMVRWVSR
                     VADDIDDRLLAEFGGTSKRRDG"
     gene            complement(1919..>2319)
                     /locus_tag="PLY56_15710"
     CDS             complement(1919..>2319)
                     /locus_tag="PLY56_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22973.1"
                     /translation="RRGEPGWGCFAGRRGGYAADMNDSPVGFQEDWAAHFARRMAQRI
                     AHAGRTRDLPARRDALEEALDLGKEARLRIQAGYTETAEESAALAHLQAALDEVQQLL
                     NAIDPCYEPMLASAQAHAEAAADALNAAGR"
BASE COUNT          374 a          780 c          772 g          393 t
ORIGIN      
        1 gcctccgaga acgccacgcg gtggccttcc tccctggggc ccgagggcag ggatgaggtc
       61 aggcggttgg agatccgggg gagcgcaagc aaccgctcga atcggccttc tctcggaatc
      121 ccagttcagg ccgggttttc cttgacccgg gatgcggggg cgtgctatac tgaggcgagt
      181 gccgcgggca gtgccgccgc ggtagaccgt cgtggtgttc tcaggagatg ccggaggatg
      241 gaagcccgcc tcaaacccgc cgtggcccgc atcctggatg agctccgctc tcgcccgaag
      301 ggcatcaaaa agatgtcggc cagacgcctc ctgcgcgcgt ttggctacga gcggcgtagc
      361 caggagaatg tgcgcgagat acgtgcggag ctcgcgcgtg ccaggatcga ggtcaacctg
      421 gccgtggatg acccgaaaac cctggatgag ctggtcactc tgcggctacg caaggcgaca
      481 cgggcgcttc cggttgatgc gagtgacgca ccccctgccg gggggaagac ggttggcgac
      541 cggccgctct cgccgctgcc caccccggcg cagatcgcgc aaggccggca ccgggatatc
      601 accgacgtca ttgcagccgt caaacagtgc acggtacaga tcttcacgga gatcggcgag
      661 ggctccggtg tcatcgtgga tcccaatggc ctcatcctca ccaaccggca cgtggtcgat
      721 accgaggagg gtctctccga gcgctacgtt cacgtgcatt tcgccgatgg cactgaggat
      781 gacgcgacgg tggttcgctc gcaccgcccc ctcgactacg cgcttctcgt ggtgccacgc
      841 cagggcctat gtgcggtgcc gctgggcgat cccctcaagc tcaaggatgc gaagacgctc
      901 tacgccatcg gcaaccccgg ggggctggcg ttcagcgtca cccggggaat cgtcagcgcc
      961 gcgcgccgcg tctgcacgac caacgtggag tacatccaga cggatgcggc gatcaacgcc
     1021 ggcagctccg gcgggccagt ggtgaccgaa cagggtgatc tcgtcggcat cagcgcgtgg
     1081 gcgtatcgtg gcgatgggcg tttgcaggca caggggatca acttcgccat ccccgtcgac
     1141 tacatgcgtg aggagatacg cgcgttgcgg gccatcggtt tccaggaggc ctgtcgtcgc
     1201 tactactgcc gccactgcgg ctggcttgaa gagagcgaag gccgctactg cagcaactgc
     1261 ggggccgcgc gtaccgaagg gagcgagagg tgaggcctcc tgctgcacat caccgtctgg
     1321 gcgtgagcct gctccagcag cgcgagagag gcgtgccgca gctggcggag caccacgctc
     1381 gcaccccgga gcaacgggag acactgcgcg cgcagggcgc gcaggccatc atggatggga
     1441 tgaagcgcct gggagcttcc gatcccaccg cgctccagga tgagaacggc ttcctccatt
     1501 tcccggtggg atcgattcag tgcacgctgg gcatcgtcac cctggaggag cgcgaccttt
     1561 tcctagtgta cgtccccata atggacctgc cttcggatcg cgacctcatc ctcccgctct
     1621 accgcctcct tctggagctg aacaaccgtt tcgagctttc catcgccaag tttggcctgc
     1681 acggcgatac catcttcctc agcgccatgc gccccctgga cagcctggat cccaccgagg
     1741 tggaggatat ggtgcgctgg gtttccaggg ttgccgatga tatcgatgac cgcctcctgg
     1801 ccgagttcgg cggtacgtcc aagcgccggg acgggtaggg cgccgcgccc tcacgcgatg
     1861 cgggcgcgcc gatgggggct ttgccgaatg accaccgatt attgggcccg ccgggtggct
     1921 accttcccgc tgcgttgagc gcatcggcgg cggcttccgc gtgtgcctgc gccgaggcga
     1981 gcatcggttc gtagcagggg tcgatggcgt tcagaagctg ctgcacctca tccagcgccg
     2041 cctgcaggtg cgccagcgcc gcactctcct cagcggtctc ggtgtatccc gcctgaatcc
     2101 ggaggcgcgc ctccttgccc agatccagcg cctcttccag cgcgtcacgc cgggccggca
     2161 ggtcgcgcgt tcgccccgcg tgcgcgatcc gctgtgccat tcggcgcgcg aagtgtgcgg
     2221 cccagtcctc ctgaaagccc accggggagt cgttcatatc ggccgcatac cctccgcgac
     2281 ggcccgcgaa acacccccag cccggctcgc cgcgccgct
//
LOCUS       DAOSCV010000744         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30309_length_2318_cov_2.738842, whole genome shotgun sequence.
ACCESSION   DAOSCV010000744 DAOSCV010000000
VERSION     DAOSCV010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30309_length_2318_cov_2.738842"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1097)
                     /locus_tag="PLY56_15715"
     CDS             complement(27..1097)
                     /locus_tag="PLY56_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22974.1"
                     /translation="MRYFLLIGLLMIGGAARSAPLTLLPNQDFEDAQLGWSLWPTESQ
                     SRLEIDREVVRNGRQSLRVTAVRSSDRAFAVIATPNFEHEVVYRISVAIRKDASVPDS
                     AISYAINYRGGDNNAILQRARPTQLRRVPEGEWVRWSGLFMTPKGVREWQFLLGVEYA
                     VGRVWFDDIRIERLGPASELNPDVWTNLTVGVEIGSGPLQRFTKHKEENNTIYRMSKR
                     YNDLLLRSAFAEAELRELERCLHYAGKPGPARARRLFEATEARLNEAFLAYGTAFRSG
                     KEGEWAAFEKSAAALEKALADLEAGVAREKAEVRPAKPPALPKWLGRQSRDIAPIRPD
                     GRMNRLLFGVWSPTAFAEYERP"
     gene            complement(1177..>2318)
                     /locus_tag="PLY56_15720"
     CDS             complement(1177..>2318)
                     /locus_tag="PLY56_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22975.1"
                     /translation="AQKLDLHREGGVGHRVVEPAVHEGWVAICPAFPSDWTEASIRTP
                     VVSYEYRREGSQAVFHIRSPKPLVKRVRAYPGGREVVTPAETESVVTVPLAAVPPLPA
                     AGPRPQLLVEHPGALDGEEPAAGPKVAAALAPSQVPLTEAERARQILFDLSGAANVTL
                     EEMTAMRFQFEGGEGLASLSSGWGNPPLAMRPSPRVLEVSNGVRFLTGGRIPRSLGYT
                     PKNLLALSSWAPYPLPAGATIQVMRRCERLWLLLQSYVHPTKNYVPNGEVVLRYADGT
                     RKILSLVPPYNLDCYFQHFSLDGMPVPFGYLGAEPAGWTPLYPERTRAHADVLAIESD
                     PTRLLEAVELRATCSEGILGLAGMTALAAEDEPSAGLAAGQSPSR"
BASE COUNT          368 a          804 c          778 g          368 t
ORIGIN      
        1 ccggatgggc ggatgaaccg cctcctctac gggcgctcgt actccgcgaa cgcggtgggg
       61 ctccaaacgc cgaagaggag gcggttcatc cgcccatccg ggcgaattgg ggcgatgtcg
      121 cgcgattgtc gacccagcca tttcggcagc gcgggcggct tcgccgggcg aacctcggcc
      181 ttctcgcgcg cgacgccggc ttccaggtca gccagcgcct tctccagggc ggcagcgctc
      241 ttctcgaaag ccgcccactc cccttccttg ccagagcgga acgccgttcc atacgccagg
      301 aatgcctcgt tcaggcgcgc ctcagtcgcc tcgaatagac ggcgcgcccg cgccgggccc
      361 ggcttccccg cgtagtggag gcagcgctcc agctcgcgca gctccgcctc ggcaaacgcc
      421 gaccggagga ggagatcgtt gtaccgcttt gacatgcggt agatggtgtt gttctcctct
      481 ttatgcttcg tgaagcgctg gagcggcccg gagccgatct ccacgcccac cgtcaggttg
      541 gtccagacgt ccgggttcag ctcgctcgcc gggcccaggc gttcgatgcg aatgtcatcg
      601 aaccacacgc ggcccacggc gtactccacc ccgaggagga actgccactc gcggaccccc
      661 ttgggcgtca tgaagaggcc cgaccagcgg acccactctc cctcgggcac gcgccggagc
      721 tgcgtcggcc gggcgcgctg caggatggcg ttgttgtctc caccccggta gttgatcgcg
      781 tagctgatgg cagagtcggg cacgctggcg tccttgcgga tcgccaccga gatccgatag
      841 acgacctcgt gttcaaagtt gggggtggcg atgacggcga aggcgcggtc gctactgcgc
      901 acggcggtca cgcgaagcga ctgccggccg ttgcgcacca cttcccggtc gatctccagg
      961 cgcgattggg actcggtggg ccagaggctc caaccgagct gggcatcctc aaagtcctga
     1021 ttgggcaaca gggtgagcgg cgcgcttctg gcggcaccgc cgatcatcaa caagccgatg
     1081 aggaggaagt atctcatagt caggctctcc tcgcaagagg gcctctgtcc cgatgccaga
     1141 ggccctcttg ccaccgttct gtcgtttagc gcacgctcat cgcgagggcg actgtccggc
     1201 ggcaagaccg gcagaaggct catcctccgc ggccagggcg gtcatgcccg ccaggccgag
     1261 gatcccctcg gagcacgtcg cgcgcagctc caccgcctcc aggaggcgcg tgggatcgct
     1321 ctcgattgca agcacgtcgg cgtgggcgcg cgtcctctcc ggatagaggg gcgtccagcc
     1381 agccggctct gctcccagat acccgaaggg cacaggcatc ccatccaggc tgaagtgctg
     1441 gaaatagcag tccaggttgt agggcggcac gagcgagagg atcttccgcg taccatccgc
     1501 gtagcggagg accacctcgc cgttcggcac gtagttcttc gtcgggtgaa cgtagctctg
     1561 cagcagcagc cagagacgct cgcaccggcg catcacctga atcgtggccc cagctggcag
     1621 cgggtagggc gcccagctgg agagcgccag gaggttcttc ggcgtgtagc ccagcgagcg
     1681 cgggattcgg cccccggtca ggaaacgcac gccgttgctc acctccagga cgcgcggcga
     1741 cgggcgcatc gcgagcggtg ggtttcccca gccgctggag agggatgcga gcccctcgcc
     1801 gccctcaaac tggaagcgca ttgcggtcat ctcttcgagg gtgacgttgg cggcgccgga
     1861 gagatcaaac aggatctggc gcgcgcgctc ggcttcggtc agcggcacct gcgagggggc
     1921 cagcgcggcg gccaccttcg gtccggcagc gggctcctct ccgtctagcg cacccgggtg
     1981 ctccaccaga agctgcgggc gcggtcccgc agccggcaag gggggcaccg ccgccagggg
     2041 caccgtgacc accgattccg tctccgccgg ggtgaccacc tcgcgcccgc ccggataggc
     2101 gcgcacgcgc ttcaccagcg gcttcggcga ccggatatgg aagacggcct ggctcccttc
     2161 acgccggtat tcatagctca cgaccggcgt gcggatgctg gcttcggtcc agtcgcttgg
     2221 gaaagccggg cagatcgcca cccaaccctc gtgcaccgcg ggctcgacga cccgatggcc
     2281 gacgccgcct tcccggtgaa gatcgagctt ctgcgcga
//
LOCUS       DAOSCV010000745         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30337_length_2316_cov_3.434763, whole genome shotgun sequence.
ACCESSION   DAOSCV010000745 DAOSCV010000000
VERSION     DAOSCV010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30337_length_2316_cov_3.434763"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1637)
                     /locus_tag="PLY56_15725"
     CDS             complement(207..1637)
                     /locus_tag="PLY56_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOJ22976.1"
                     /translation="MERDVVPFIVNGQPVRTGRSFPVINPATEERIAEVANADRASVE
                     AALAAARDAFPGWAATPIAERRAIMDRCCAVLEREKERLVDLLVAETGKPYDTAAYGL
                     SCLVGSLRFFSEEAQRVSQEVIPDPTGRFLHYVLRQPLGVVVGLLAWNYPLLNLGYKL
                     GPVLASGCTAVIKPSRHTPLATLAAVELLAEAGVPAGVVNVITCDDHRVSNALLESRI
                     PSLITMIGSTEAGVEVMATAATSIKRFSLELGGNAPAIVYPDADLTDAAIRIVNLKFS
                     NAGQVCVAPNRCFVHAKVYEEFLSLAAERAGAITLGSGKGPMPMMGPLMTAEARDAAL
                     ELVRESLDAGAKLVCGGRKPEQPARGYFMEPTILRDVRPEMRVARQEIFGPILAVIPF
                     DDEQDLLAMANDTEHGLAAFVFTSDLSTALRAAEGLQAGSVCINEPHFGIHLPHAGQK
                     QSGVGMDRSCHSLEEYLTVKRVSIRK"
     gene            complement(1757..>2316)
                     /locus_tag="PLY56_15730"
     CDS             complement(1757..>2316)
                     /locus_tag="PLY56_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HOJ22977.1"
                     /translation="HLGPEVDLALDCHWAFDLPGALRLGRALEPYHLMWFEDPIPSGN
                     LAALARLASELSVPICIGEALCTRFQFREVFERGAASIIMPDVARAGGILEMKKIAAM
                     ADTYYIPVAPHDMVGPIATAASLHVCASIPNFLILEHQMNDVPWRDELTDEPLVVRDG
                     QMELPTRPGLGVRLNYAAVEKYRAE"
BASE COUNT          391 a          808 c          746 g          371 t
ORIGIN      
        1 gcccacactc catcgttccc gggcaatagc ttgcagagct gttcctccga gagcgaaccg
       61 gtacactccg ccaggatcac ctctatcccg gccctctcga actcctcgag aatatccggg
      121 gccgtcaccc gcggcgaaag cgcggtgacc agcaatcgaa agccccctgt gtcgcccatg
      181 gcactcctcc acccgtccgg cgccggctac ttgcggatgc tcacccgctt caccgtcagg
      241 tactcctcca ggctatggca cgagcggtcc atgccaaccc cgctctgctt ctggcccgcg
      301 tgcggcaggt ggatcccgaa gtgcggctca ttgatgcaca cactccccgc ctggaggccc
      361 tcggcggcgc gcagggccgt ggataggtcg cttgtgaaga cgaatgcggc caggccgtgc
      421 tccgtgtcgt tcgccatggc gaggaggtct tgctcgtcat caaaggggat gaccgcgagg
      481 atcgggccga aaatctcctg gcgggccacg cgcatctccg ggcgcacgtc gcgcaggatc
      541 gtcggctcca tgaagtagcc gcgcgccggc tgctccggct tgcggccgcc gcacaccagc
      601 ttcgcgcccg cgtcgagcga ctcgcgcacc agctccaggg cggcatcccg cgcctcggcg
      661 gtcatcagcg gacccatcat cggcatggga cccttgccgc tgccgagcgt gatcgcgccg
      721 gcgcgctccg cggccagact gaggaactcc tcatacacct tcgcgtggac aaagcagcgg
      781 ttgggtgcaa cgcacacctg gccggcgttg ctgaacttca ggttgacgat gcggatggcg
      841 gcatcggtga gatcggcatc cggatagacg atcgcgggcg cgttcccccc gagctccagc
      901 gagaagcgct tgatgctcgt cgccgccgtc gccatcactt ccacgcccgc ctccgtcgag
      961 ccgatcatcg tgatgagcga cgggatgcgg ctttcgagga gcgcgttgga gacccggtgg
     1021 tcatcgcacg tgatcacgtt cacgacgccg gcgggcacgc ctgcctccgc cagtagctcc
     1081 acagcggcga gcgtcgccag tggggtatgc cgcgaaggtt tgatcaccgc cgtgcacccc
     1141 gaggcgagca ccgggcccag cttgtagccc aggttcagga gggggtagtt ccatgcgagc
     1201 aagccgacaa ccacgccgag cggctggcgc aggacgtagt ggaggaagcg gcccgtggga
     1261 tccgggatca cctcctggct cacgcgctgc gcctcttccg agaagaagcg gagcgagcca
     1321 accaggcagc tcaatccgta ggcggcggtg tcgtacggct ttcccgtttc cgcgaccaga
     1381 agatcgacca ggcgctcctt ctcccgctcc agaacggcgc agcagcgatc catgatcgcc
     1441 cggcgctctg caattggcgt ggccgcccac ccgggaaagg catcgcgcgc cgccgccagc
     1501 gccgcctcga cacttgccct atcggcgttc gccacttccg cgatgcgctc ctccgtggca
     1561 gggttgatta ccgggaagga acggcccgtg cgcaccggct ggccgttcac gatgaaggga
     1621 acaacgtcac gttccatgct actctcccgc gggcctcaga gaagcgccct gcatagcagg
     1681 aggaagaggc ccgccatgcg gtcgccatcc tgtgcctcgg atccgcgagc cctgaagccc
     1741 cgttcgctga tgccggttac tccgcgcggt acttctccac ggcggcataa ttcaggcgga
     1801 cgcccagccc cggccgcgtc ggaagctcca tctgcccgtc tcgcacaacc aacggctcgt
     1861 cggtcagctc atcacgccag ggcacgtcgt tcatctggtg ctccaggatg aggaagttcg
     1921 gaatggacgc acagacgtgc agtgaggccg ccgtggcgat cggcccgacc atgtcgtggg
     1981 gcgcgacagg gatatagtag gtatctgcca tcgccgcgat cttcttcatc tcgaggatgc
     2041 ccccggcgcg cgccacgtcg ggcatgatga tgctggctgc cccgcgctcg aacacttcgc
     2101 ggaactggaa gcgggtgcag agcgcctcgc cgatgcagat gggcaccgag agctccgagg
     2161 ccagtctggc caacgcggcg agattgccgg aggggatggg gtcttcgaac cacatcaggt
     2221 gatacggttc gagcgcgcga cccaggcgca acgccccggg gaggtcgaac gcccagtggc
     2281 agtcgagcgc caggtcaacc tccggcccga gatgct
//
LOCUS       DAOSCV010000746         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30339_length_2316_cov_3.216276, whole genome shotgun sequence.
ACCESSION   DAOSCV010000746 DAOSCV010000000
VERSION     DAOSCV010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30339_length_2316_cov_3.216276"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLY56_15735"
     CDS             complement(<1..478)
                     /locus_tag="PLY56_15735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ22978.1"
                     /translation="MQEDIKVLIVDDSEQTRETLRNILSLEKEIQVVGEASDGQEAVQ
                     RVAELHPDVVIMDVNMPGVDGIQATERITTDHPDSCVIVMSAAGDQEYLRKAMQAGAR
                     DYLIKPFTSDEVRSMATAVVDDVGVEALQAAGITPEDVRAAGDITVAGLVDQAGAEA"
     gene            complement(507..1946)
                     /locus_tag="PLY56_15740"
     CDS             complement(507..1946)
                     /locus_tag="PLY56_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HOJ22979.1"
                     /translation="MVRLRMVEGKSRILPTQGVRTIQIDDPSGAVAEVQPVSDTEILL
                     LAKGPGVTMVRVWDRRGRTEYELTVEPGPARRQQMIQEAINDSKITVRVVENTCILEG
                     EVGSVEQMMRAQRIAEAFSVQVVNLLQLPAPPPAQPPAPGGPSLLEQLQAVLPAGEVT
                     AELLPGQPTTVVLRGQAPRVEDIDGLVAIAEKVVAPVNGTVINLVQLAQPRQVRVQAR
                     MLNVDSRRLRELGVRWMDTSTTGPTLFGQTTPTGGFQLLTSIQANIKTLLEDAHAELL
                     SAPSTVVNSGGTGYIHIGGEVPIPQLSGATGGTDSVLTTTVTYRNFGVLLGIEPVAEP
                     GDEITLRLLVEVSSIDRNNAVTVNGSEIPGFSTKRALNELRLKAGETLVVGGLISHEE
                     ASTVHRFPILADIPIFGSFFRSIRKEKFARELVLFLTPELLPGPEPPAGLRQTPVLKP
                     GGGSTNPFNLSTSTSMQGSGVGTSSGGTR"
BASE COUNT          363 a          776 c          745 g          432 t
ORIGIN      
        1 atgcctcggc accggcctga tcgaccagtc cggccaccgt gatgtcgccc gcggcgcgca
       61 cgtcctcagg ggtgatgccg gccgcctgca gcgcctcgac tcccacatcg tcgacgactg
      121 cggtggccat gctgcgcacc tcgtcactgg tgaacggctt gatgagatag tcgcgtgcgc
      181 cggcctgcat cgccttgcgg aggtactcct ggtctcccgc cgcggacatg acgatcacgc
      241 aggagtcggg gtggtcggtg gtaatgcgct cggtcgcctg gatcccatcg acgcccggca
      301 tattcacatc catgatgacc acgtcaggat gaagctcggc cacccgctga acggcctcct
      361 gaccgtccga cgcctcgccg accacctgga tctccttttc gagggagaga atgtttcgca
      421 acgtctccct cgtctgctcg gagtcatcca caatcaggac tttgatgtcc tcttgcatcg
      481 ccttctacac gctcctttgg cggccgctat cgggtgccgc cgctgcttgt tccaacacca
      541 cttccctgca tggatgtgct cgtgctgagg ttgaacgggt tggtggaccc accgccgggc
      601 ttcagtacgg gagtctgccg cagaccagca ggcggctccg ggccgggcag gagttccggc
      661 gtcaggaaaa gcaccaactc gcgcgcgaac ttctccttgc ggatcgaccg gaagaagctg
      721 ccgaagatcg ggatgtcggc caggatcggg aacctgtgca cggtggatgc ctcttcgtgc
      781 gagatcaggc cgcccaccac cagagtttcg cctgccttta ggcgaagctc gttcagcgcg
      841 cgcttggttg agaagccggg gatctcgctg ccgttgacgg tgaccgcgtt gttgcgatcg
      901 atgctgctta cctccaccag aagccgcagc gtgatctcat cgcccggttc ggccaccggc
      961 tcgatgccga gcaggacgcc gaagttgcgg taggtcaccg tcgtggtaag taccgagtcg
     1021 gtgcctcccg tcgctccgct cagttgtggg atcggaacct cgccgccaat gtgaatgtag
     1081 ccggttccgc cactattgac caccgtgctg ggtgccgaga ggagttcggc gtgtgcatcc
     1141 tcgagcagag ttttgatatt ggcctggatg ctggtcagca gctggaaccc gcccgtcggc
     1201 gtcgtctgcc caaagagcgt tggcccggtg gtggaggtat ccatccagcg cacgcccaac
     1261 tcgcggagcc gccgcgagtc cacattcagc atgcgcgctt gaactcgcac ctgccgcggc
     1321 tgggccagct ggaccaggtt gatcaccgtg ccgttgacgg gagccaccac cttctctgcg
     1381 atcgcgacca ggccatcgat atcctcaacg cgaggggcct gcccacggag caccaccgtg
     1441 gttggctggc cggggaggag ctccgccgtt acctcgccag ccggaaggac cgcctggagt
     1501 tgctccaaaa ggcttggccc gccgggggcc gggggctgcg ccggcggtgg cgcgggtagc
     1561 tgcagcagat tgaccacctg gaccgagaac gcctccgcga tcctttgggc gcgcatcatc
     1621 tgctctacgc tgcctacctc gccttctagg atgcaggtgt tctccacgac gcgcacggtg
     1681 atcttgctat cattgatggc ttcctggatc atctgctgcc ggcgcgccgg acccggctcc
     1741 actgtgagct catactcggt gcggccgcgt cgatcccaga cgcgaaccat tgttaccccc
     1801 ggacccttcg ccaggagcag gatctcggta tcactcaccg gctgtacttc agcgacggcg
     1861 cccgacgggt cgtcaatctg gatggtgcgc acaccctgcg ttggcaagat gcgcgatttg
     1921 ccctccacca tgcgaagacg taccacccca tcagatggct cggcaccagg gtgagcgggc
     1981 tctggggcga gaggggtcgc gtctgaaacg gcgcgagccc ccgcgagcag ggtgatggcg
     2041 tccctcgctc cctgggccac catctgctca cggggcgcgg cgatgcgcac gaccgggttg
     2101 gatactcgcg ccccggccgc atctttaagg gccggcgtgg tctgcgatac cggtgccacg
     2161 gcatcggggg gcctgtgtgc cccggccccc gtcggaaccg tgaggcgcca gccgcttggc
     2221 gttgctttca ggcgcggagc acggacgagt cgcgatgcga agaccggcac gcatgggacg
     2281 cgcctgctgc cttccaacac cgatcggggc gcgctc
//
LOCUS       DAOSCV010000747         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30356_length_2315_cov_3.260619, whole genome shotgun sequence.
ACCESSION   DAOSCV010000747 DAOSCV010000000
VERSION     DAOSCV010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30356_length_2315_cov_3.260619"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLY56_15745"
     CDS             complement(<1..405)
                     /locus_tag="PLY56_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22980.1"
                     /translation="MKLNNHEAPRRNETLAERIARHARALGYEADVSCSRLSGSAYVD
                     ITTKDGAAVARVRLANHEERPSYAGLHGRVTHYIDTTPGTSGLTWHDVVVELATRAGK
                     PVPASIRAAQAAAKTRAAKRQADLAARRKAYAN"
     gene            complement(402..869)
                     /locus_tag="PLY56_15750"
     CDS             complement(402..869)
                     /locus_tag="PLY56_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22981.1"
                     /translation="MDAYTIVATEFDCGHTYSGRQPSHDACPVCVQAIAARLLAVARA
                     EALASAQREVQRLSDDGLIQVAVDESQGDASRVVLALERRLLWNAERSLCVQREAALR
                     AAGALPPLPDWAKYVVDMITVHYYMGSSGTYSALEAEYLVRRHAGLNPQEAPR"
     gene            complement(1466..1744)
                     /locus_tag="PLY56_15755"
     CDS             complement(1466..1744)
                     /locus_tag="PLY56_15755"
                     /inference="COORDINATES: protein motif:HMM:NF021190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HOJ22982.1"
                     /translation="MRIIVSQHPAAVEFIRRELPEFSGAPVLTTATPDDVRGRVVAGN
                     LPLHLAALAAEVIGIQFAGPPPRGREYGIQDMYAAGAYLARYRVEALS"
     gene            complement(1832..2167)
                     /locus_tag="PLY56_15760"
     CDS             complement(1832..2167)
                     /locus_tag="PLY56_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22983.1"
                     /translation="METYKAHNLAASEVEMSFGTLRAIVLGLVAVGMNARQRRRVIEE
                     MHCADYDTVGEAAFWALPCDRDNKVLHDLDGWPKCACTACGCDEPATCTDDGGVEVCS
                     TCHRCPSSR"
BASE COUNT          385 a          753 c          781 g          396 t
ORIGIN      
        1 gttggcgtag gctttgcggc gtgcggcaag atcagcctgc cgctttgctg ctcgggtctt
       61 agcggcggcc tgagctgcgc ggatgctggc cggtacgggc ttccccgcac gcgtggccag
      121 ttcaacaacc acgtcgtgcc aagtcagccc gctggtaccc ggcgtcgtgt cgatgtagtg
      181 cgtcactctt ccgtgcagcc cagcatagct aggccgctct tcgtggttgg cgagtcggac
      241 gcgcgccacc gcagcgccgt ccttggtggt gatgtctaca tacgctgagc cagataacct
      301 gctgcacgag acatcggctt cgtaaccgag tgcccgtgcg tggcgggcga tccgctcagc
      361 aagggtttca ttacgccgtg gcgcttcgtg gttgttgagc ttcatcgtgg cgcctcttgg
      421 gggttcagtc cggcatgccg gcggaccaag tactccgcct ccaaggcgga gtaggtcccg
      481 gaggaaccca tgtagtagtg gacagtgatc atgtccacta cgtacttagc ccagtcgggc
      541 agtggcggta gggcgccagc agcacgcaga gccgcctcgc gctggacgca gaggctccgc
      601 tctgcgttcc atagcagacg cctctccaga gcgagcacca ccctgctcgc gtcgccctgg
      661 gactcgtcca cggcgacctg gatgaggccg tcgtcgctca agcgctggac ctcgcgctgg
      721 gcgctagcca gcgcttccgc gcgtgccacg gcgagcaggc gcgcggcgat cgcctgcacg
      781 cacaccgggc aggcgtcatg cgacggctgc cggccggagt aggtgtggcc gcagtcgaat
      841 tctgtggcca cgatggtata ggcgtccacc cgggttccgc gctcatcaga ggagaggtac
      901 ggggcggacc gatactctcc ctcatgcctc catccggaga aatgggcacc cggagtggtc
      961 ggggcttccg ggatttcgag ggcgcgagtc aaaaatcgct cgcgccagga aaagatggct
     1021 ggccgcgcgg cctctacagc cgcccggcgc tcggcctcca cctgctgctg atagcagtgg
     1081 aggcacaacc cacgtcggca ggtggactga cgccagtcgc ccagcggctg tccgcacgct
     1141 gggcacggga gggaattgcg ccgctgcttc tctgcggcgc aaacctgaca ctccctggag
     1201 tggacgtgct ccaggagccc aaggtccggg tctccagcac tccactctcc gggccagagc
     1261 ccagcagcga tgtaagcccg ccccgaagcg gtcgggcgaa gggacgcagc tacgtagggc
     1321 tgcccctgct ccacctgctc accacgggcg gggaggatga tctttccgtc tgggtgacgg
     1381 gcgatgagcc cgtcacgacc aggacggaaa tgaagcgtaa gggggagcat aacatgctcc
     1441 ggaccaaggg gaaggggcga ggcgctcatg agagcgcctc cacgcgatag cgggccaagt
     1501 acgccccggc ggcgtacatg tcctggatgc cgtattcccg gcctcgcggt ggcgggccgg
     1561 cgaactggat gccgatcacc tcggcggcca aagccgcgag gtgcaggggc aagttgcccg
     1621 cgaccacgcg gccgcgaaca tcgtccgggg tcgcggtcgt caacacgggc gccccggaga
     1681 actcgggcag ctcgcggcgg atgaactcca ccgcggcggg atgctgtgac actatgatcc
     1741 tcatggttta gctcctccat ctggccgtcc gtccgtatcg ttcgggcggc caggtggagg
     1801 cccggatatc gctccgggcg gggcggaggg gttagcgtga ggaggggcat ctatgacagg
     1861 tggagcacac ctcgacccca ccatcatcgg tgcatgtggc cggctcgtcg cagccgcatg
     1921 cggtacaggc gcacttgggc cagccgtcca ggtcatgcag aaccttattg tcccgatcgc
     1981 acgggagagc ccagaacgcc gcttcgccga ccgtgtcgta gtcggcgcag tgcatctctt
     2041 cgatcacccg gcggcgctgc cgggcgttca tgccgaccgc gacaaggccg aggacaatag
     2101 ccctcagcgt tccgaatgac atctcgacct cgctcgcagc gaggttgtgt gccttatagg
     2161 tttccatgat ttacctccat ctggccgtcc gccgatccat gcgtcgggcg gtcaggtgga
     2221 agaccccgat atcgctcggg gcgggcggat tcactaccga attgggagcc aacgctcgtc
     2281 aatccaagct cggatgtccg ggacatcctc acccg
//
LOCUS       DAOSCV010000748         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30357_length_2315_cov_3.253540, whole genome shotgun sequence.
ACCESSION   DAOSCV010000748 DAOSCV010000000
VERSION     DAOSCV010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30357_length_2315_cov_3.253540"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1235
                     /locus_tag="PLY56_15765"
     CDS             375..1235
                     /locus_tag="PLY56_15765"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOJ22984.1"
                     /translation="MASEEGYRKLTPEEERVIVYKGTEPPFTGEYNDHFEPGLYACRR
                     CGALLYRSEDKFHSGCGWPSFDDEIPGAVVRMSDPDGRRTEILCANCGAHLGHVFTGE
                     GFTPKNTRHCVNSISMVFVPENEAKLGRAIFAAGCFWGVEHCFKEQVGVVRTTVGYTG
                     GWKENPTYEEVCSGQTGHLEAVEVLYDPARLTYEALAKLFFEIHDPTQVDGQGPDIGE
                     QYRSAIFYEDESQKQIAEKLIRELEAKGLRVATRVLPASRFWPAEEYHQDYYEKKGSA
                     PYCHFRQPRW"
     gene            complement(1326..2057)
                     /locus_tag="PLY56_15770"
     CDS             complement(1326..2057)
                     /locus_tag="PLY56_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ22985.1"
                     /translation="MWHSRRGFTLIELLVVIAIIAILAAILFPVFARARENARKAACQ
                     SNLKQISSGLLMYVQDYDETLPIVTTAITGGYRMSDPDVPCCSKAWFQNKSATNPAPR
                     PSMVHNGFVAYRLEPYVKNAQVWRCPSMSGTPDVAADSTSYLCSLSISQRYSAATLLL
                     EGTPLAALKPSPAEIPIFQDAVGWYEPGTAANLYRGSWAVEGWSSPHGTGGGSPTNVA
                     FLDGHVKALPIHRWMLAIRDTQPWK"
BASE COUNT          412 a          705 c          789 g          409 t
ORIGIN      
        1 cgacacggac gggtgctcca cgacgcggcg gcgcaggtcc tccgtgaaac tggcgtgata
       61 gaggattcgc cccggacgcc ggtgcaccgt agtcgccagg aagttctctc gatcggtcgc
      121 cattggttgc tctctccatg ttgctagtgc gggatgcgtg ataccgcgcg tggccccgcg
      181 cgctcctccc tcaattcggt ccggagcggg gtctctcctc gtacgctgat ccaccggatc
      241 ggccctgcag ctccattcgg gtgtcacggg gcaccgggtt ggagtgtcaa accttccgag
      301 gggcttcacg gaagcggcca taccgggtaa actggggcga atcagcccga tctcgacaag
      361 gaagggagga gaagatggca tcggaagagg gataccggaa gctgacgccc gaagaggagc
      421 gcgtcatcgt ttacaagggg accgagccgc cgtttacggg ggagtataac gaccacttcg
      481 agcccgggct gtatgcctgt cggcggtgtg gcgcgctgct ctatcgctcg gaagataagt
      541 ttcactcggg ttgtggctgg ccgtcgtttg atgacgagat ccccggcgcg gtggtacgca
      601 tgtcggatcc cgatggccgg cgcaccgaga tcctatgcgc caactgcggc gcgcatctcg
      661 gccacgtctt cacgggtgag ggctttacac cgaaaaacac ccgccactgc gtcaactcca
      721 tctcgatggt ctttgttccg gagaatgagg cgaagctcgg gcgggcgatc tttgccgccg
      781 gttgcttctg gggcgtggag cattgcttca aggaacaggt gggcgtggtg aggacgaccg
      841 tgggctatac cggcgggtgg aaagaaaacc ccacctacga ggaggtctgc tcgggccaga
      901 ccggccacct cgaggcggta gaggtcctct acgacccagc gcgcctcacc tacgaggcgc
      961 tcgcgaagct cttcttcgaa atccacgacc caacgcaggt ggatggccag ggcccggata
     1021 tcggggagca gtaccgctcc gcgatcttct acgaggacga atcgcagaag caaatcgccg
     1081 agaagctcat ccgggagctg gaggcgaagg ggctccgggt ggcgacgcgg gtgcttccgg
     1141 cctcccgctt ctggccagcc gaggagtatc accaggacta ctacgagaag aagggctcgg
     1201 cgccctactg ccacttccgc cagccgcggt ggtagcatcc ctccctggcg ggaggaaccg
     1261 atggcggctg cctaacccag aggcgggcag ttctggcgcg gccgcttcgg cttgccggcg
     1321 ccgggctact tccagggctg agtgtcgcgg atcgcgagca tccagcggtg gatcggcagc
     1381 gccttgacgt ggccatccag gaaggcgacg ttcgtggggc tgcccccgcc ggtgccatgc
     1441 ggcgagctcc atccctcaac ggcccagctg ccccggtaaa gattggcggc ggttcccggc
     1501 tcgtaccagc ccaccgcgtc ctggaagatc gggatctccg cgggagaggg cttgagcgcg
     1561 gccaggggag tgccttccag caggagcgtg gcggcactgt acctctggga gatggagagg
     1621 ctgcacaggt aggaggtgga gtcggctgcc acgtcggggg tgccggacat cgaggggcag
     1681 cgccacacct gcgcgttctt cacgtagggc tccaggcggt aggcaacaaa cccgttgtga
     1741 accatcgacg ggcgcggcgc ggggttcgtg gcgctcttgt tctggaacca ggccttcgag
     1801 cagcacggca cgtcgggatc gctcatccgg tagcccccgg tgatcgcggt cgtgacgatt
     1861 gggagcgtct cgtcatagtc ctggacgtac atcagcaggc cgctgctgat ctgcttcagg
     1921 ttcgactggc aagctgcctt gcgcgcgttc tccctggcgc gggcaaagac cggaaagagg
     1981 attgccgcca agatggcgat aatcgcaatg acaaccagaa gctcgatcaa ggtgaaacca
     2041 cgtcgagagt gccacatgtc gtgcctccct cctccgccag agcgcccctt cgggcgccac
     2101 gcgggcggtt ccgcttgatc cggccggcgg tccggcgcga acctggtgtg agagttggca
     2161 tagtgtagca ttccgggcgt tagcgtgtca agcggttgat cgcgataacc gcgatatccg
     2221 gcagcgcgca ccttcatgcc cgagcgtgtc gcctagcccg cattattcat cagaataatg
     2281 aaaagaagtc tcactgccag gcgtggcacg ggttc
//
LOCUS       DAOSCV010000749         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30453_length_2310_cov_3.545011, whole genome shotgun sequence.
ACCESSION   DAOSCV010000749 DAOSCV010000000
VERSION     DAOSCV010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30453_length_2310_cov_3.545011"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1677
                     /locus_tag="PLY56_15775"
     CDS             223..1677
                     /locus_tag="PLY56_15775"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ22986.1"
                     /translation="MSRQSGRNRRRVTWGRALAVGAGAGVAAWGLSRAARARPAPLPE
                     GPPHRIVVLGGGFGGTSFLSELHRRLGRQSRLHVLMVDRNNVNLFTPLLYQVATGVLD
                     PDHVTYPLRPRVRQLGARFQESVVQGIDLDARQVITDDGPIPYDTLVVSLGSVANFFG
                     MRDIAERALTLKTLGDGITLRNRIIDVFEEAAVTSDPARRAELLTFDIVGAGATGVEF
                     ATALDGLIDRVLLRDYPEIRADEVRIRVIEALDHVVPEMSPRVSHLVANRLGSRNIQV
                     LLNKSVAGLDDGRLRTRDGSLLAAGTIIWTAGVRSNPIVANLPGDHARGGRIGVNEFL
                     QLPDHPEVFCIGDNTYFADSDSPRGVPPNAPAAIQMGQATARNVERALRGWPLEPFKY
                     VPRGELLALGRNHAVVNTHGILFDGWPGWVVWRAYYLYALMDSENRAGVVTDWAFAYL
                     ARRRESGRLICAPAMAEEEGITPEAAIRMGQTNL"
     gene            2099..>2310
                     /locus_tag="PLY56_15780"
     CDS             2099..>2310
                     /locus_tag="PLY56_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ22987.1"
                     /translation="MAEKEPERDERRGQRLPVGMIAAAGAATLLGALAFNKTRERVRL
                     RAHEQVLEELAQELGAQFTPSRSGAPT"
BASE COUNT          391 a          744 c          787 g          388 t
ORIGIN      
        1 tcgccgcgcg ccgggttgcg ccctgcctcc gaggcttcgg attccttatg tcggaatctc
       61 agcagtcaac ccgccaaccg gctccggtga gagcgcattg gatgcatcgc gctggcggca
      121 tcatgcccca ctgtgcgagc gggtttcaac acccatgacg cgcggtagaa cgtctccggg
      181 ccatgcccat gggcatactc gatggccctg ggggagggcg tgatgagcag gcaatcggga
      241 cggaaccgga ggcgagtgac ctggggccgg gcgctcgcgg ttggcgctgg cgcgggggtg
      301 gctgcgtggg gcctatcacg ggccgccaga gcgagaccag ccccactgcc ggaggggccg
      361 ccacaccgca ttgtggtcct gggcggtggc ttcgggggca cctctttcct ctcggagcta
      421 caccgacgcc tgggccgcca gtcacgcctc catgtgttga tggtcgaccg gaacaacgtc
      481 aacctgttca ccccattgct ctatcaggtc gccactgggg tgctggatcc ggaccacgtc
      541 acctatccgc tccgcccgcg agtgcgacag ctcggggcac gctttcagga gagcgtcgtt
      601 caggggattg acctcgacgc gcgccaggtc atcaccgatg atgggccaat tccctacgac
      661 accctggtcg tgagcctggg gagcgtcgcc aacttctttg ggatgaggga tatcgcggag
      721 cgcgcgctca ccctgaagac cctcggcgac ggcatcacgc tgcgcaaccg gatcatcgat
      781 gtcttcgagg aggcggctgt gacgagcgac ccggctcgcc gggccgagct gctgactttc
      841 gatatcgttg gcgcaggcgc gaccggcgtg gagtttgcca ccgcgctcga tggcttgatc
      901 gaccgggtcc tcctgcgcga ctacccggag atccgcgccg atgaagtccg gatccgcgtg
      961 atcgaggcgc tcgaccacgt cgtgccggag atgagcccca gggtctcgca cctggtggcc
     1021 aatcgccttg gctcccgcaa cattcaggtt cttctcaaca aatcggtggc cggcctggat
     1081 gacgggcggc tgcgcacgcg cgacgggagt ctgctggcag ccggcaccat catctggaca
     1141 gcgggggtgc gctccaatcc gattgtcgcg aatctgccgg gcgaccatgc gcgagggggc
     1201 cggatcggcg tcaacgaatt cctccagctc ccagaccatc cggaggtgtt ctgcatcggg
     1261 gacaacacct acttcgcgga tagcgattcc ccgcgtggcg tgccgcccaa tgcgcccgcc
     1321 gcgattcaga tgggtcaggc gaccgcgcgc aacgtcgagc gcgcgctgcg cggatggccc
     1381 ctcgagccgt tcaagtatgt ccctcgtggc gagctcctgg cgctcgggcg caaccacgcc
     1441 gtcgtgaaca cgcacggcat cctctttgat ggctggccgg gctgggtggt ctggcgcgcc
     1501 tactacctct acgcgctgat ggactccgag aaccgcgccg gggtcgtgac cgactgggca
     1561 ttcgcctacc tggcgcgccg gcgagagagt ggacggctga tctgcgcgcc cgcgatggcc
     1621 gaggaggaag gcatcacccc cgaggccgcg atccgcatgg gccaaacaaa cctgtagtag
     1681 gctgggcgac actgccgcgc agaaccaggg ggtgcctccg ataccgcagt gccacaattg
     1741 gtcggtagta ggggcacctc ctggaaagag atgggtgacc cacgaccaag ggattatgag
     1801 tcccttgcac tctctcccgc ggggcagcgg cacatcctgg caagagacgt gtggccggag
     1861 agcttcagaa cggcctagcc ttcggtacct ttccactgag cggcgccagc gcatggcgct
     1921 cgcacacctc accctcccat agattcctga aacccctgga caatttgacc cctctgaagg
     1981 ggtagaggca gatgcctggt gcaaggggga gctgagcacc ccggcgtcga atgagtggtg
     2041 tacgagcagg cagcaggtaa gccgctgtgt cgtgtcgcca gcacaggagg agaagagcat
     2101 ggcagaaaag gaaccggaga gggatgagcg gcggggtcag cgtctgccgg tcggtatgat
     2161 cgccgctgca ggtgcagcga cgcttctggg cgctctcgcc ttcaacaaga cacgcgagcg
     2221 cgtgcgcctg cgcgcgcatg agcaggtgct cgaggagttg gcccaggagc tgggggcgca
     2281 gttcacgcct tctcggtctg gcgctccgac
//
LOCUS       DAOSCV010000750         2307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30520_length_2307_cov_3.010657, whole genome shotgun sequence.
ACCESSION   DAOSCV010000750 DAOSCV010000000
VERSION     DAOSCV010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30520_length_2307_cov_3.010657"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PLY56_15785"
     CDS             complement(<1..829)
                     /locus_tag="PLY56_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOJ22988.1"
                     /translation="MTSVSPEGRSFIEVQFPVAQVSAESYKERKAGASQTLTGLGKWW
                     GRKPLVMVRAALLGLLLPATADPQKDREIFFKLMTMDRDGLWRRKNRAISQTTLIEYL
                     RHMPPSVRRRFLGPSGTALQALGRQERGELERLVFEQMPYSEKIQYCLRPEQIEGPSP
                     EAWAEINAYLGTDAHSLAELVQALGRRRFGRTPRVGDAFCGGGSIPFEAARLGCEVFA
                     SDLNPMAAFLTWSALHLLHQGERAMQALRAMQEEIIKEVDRQIVEWGIEQNAQGWRAD
                     "
     gene            complement(839..>2307)
                     /locus_tag="PLY56_15790"
     CDS             complement(839..>2307)
                     /locus_tag="PLY56_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HOJ22989.1"
                     /translation="SYIARGEEGGRQWVRDFLPRMRQFLGWGEVVFETANEPDCNSNE
                     GLANLRLYSLGAMKEAEANGLRLCILNLPEGNPGDNGLGERGRSDAECRAVERWKLEQ
                     LAEAVDYAARKGHYVGLHAYWLPSENIGPLDRWHGLGRVQWNVEQWIAMGVDTSRLRV
                     FINETGVDRLISKKEREKRGWRSQAGGLDAYARQVVELERALRQLPWVEAAMLFTVGF
                     EEPWGDYDHRQDDLRVILARLKEVPMTVIYGPDATVKYWHSSREGQKIAHIVLHDTEG
                     SAEAALSWWAAPSNPYKSSAHVLVRKNGDVLHVVPEVYAAHHAGYATIPGVTVNPNLV
                     SLGLEMECSAAPNPPGYTEAQLQAAIGVVRDWMTRYGIPKERVWLHREIDAKKSDPRD
                     FDKAAFIKRLEPSELEGWLDQVPATIKAVAEMGLVPLGVEKPRQAGGYWGLGWDVLGQ
                     KYVAVGVQITSAGIEIAEMRDVAGHTVKITRSPHSIPR"
BASE COUNT          421 a          794 c          658 g          434 t
ORIGIN      
        1 catcagcgcg ccacccctgg gcgttctgct caatgcccca ctcgacgatc tgccgatcca
       61 cctccttaat gatctcctct tgcatcgcgc gtagggcctg catagcacgc tctccctggt
      121 gcaagaggtg aagcgccgac caggtgagga acgcagccat cgggttgagg tccgaagcga
      181 acacctcaca gcccaggcgc gccgcctcga aagggatgga cccgccgccg caaaaggcat
      241 ctcctacgcg cggagtgcgc ccgaagcgcc gccgccccag agcctgcacc aactcggcta
      301 ggctgtgcgc atcagtgccc aaataggcgt tgatctcggc ccaagcctcc ggcgaaggcc
      361 cctcgatctg ctccggccgc aggcagtatt ggatcttttc cgaatagggc atctgctcga
      421 acacgaggcg ctccagttcc ccacgctctt ggcggcccag ggcctgaagc gcggtgcccg
      481 aggggcccag aaagcggcgg cgcaccgaag ggggcatatg cctcagatac tcgatcaggg
      541 tggtctgcga gatggcccgg ttcttgcgcc gccacagccc atcgcgatcc atcgtcatca
      601 gtttaaaaaa gatctcccga tctttctgag ggtccgcggt ggcgggcaaa aggaggccga
      661 gaagggcggc gcgcaccatc acaagcggct tgcgccccca ccatttgccc aggccggtga
      721 gcgtctggct cgcaccggct ttgcgctctt tgtaagattc ggccgagact tgagcgaccg
      781 ggaattgcac ttcgatgaat gaacgccctt ccggtgaaac cgatgtcatt tccgttcctc
      841 aacgtggtat ggagtggggt gaacgagtta tcttcaccgt atgccctgca acatccctca
      901 tctcggcgat ctcgatgccc gccgatgtga tctgcacacc cacagcgacg tatttttgcc
      961 ctaggacatc ccagcccagg ccccagtacc ccccggcctg gcgcggcttc tctacaccga
     1021 gcgggacgag gcccatctcc gccaccgcct tgatggtggc cgggacttgg tcaagccaac
     1081 cctccaactc gctcggctcc aaccgcttga taaacgccgc cttatcgaag tctcgtgggt
     1141 cagacttttt cgcatcaatc tcgcggtgta gccacacgcg ctctttgggg atgccgtagc
     1201 gtgtcatcca gtcgcgcacc acgccaatgg ccgcctgcaa ctgcgcctcg gtataacccg
     1261 gcgggttagg tgcggcgctg cactccatct ccaggccaag cgagaccagg ttcgggttca
     1321 ccgtgacgcc tggaatggtg gcatacccag cgtggtgggc agcgtagacc tccggcacga
     1381 cgtgaagcac atcgccgttt ttacgcacaa ggacgtgcgc gctcgactta taggggttgc
     1441 tcggcgcggc ccaccaggag agggcggcct ccgccgatcc ttccgtatcg tgcagcacga
     1501 tatgagcaat cttctgcccc tcgcggctgg aatgccaata tttcacggtc gcatctgggc
     1561 cgtatataac ggtcatcggc acctctttca gtctggccag gatgacccgc aggtcgtcct
     1621 ggcggtggtc ataatcgccc cacggctcct cgaaccccac tgtgaagagc atcgcggcct
     1681 cgacccaggg gagttggcgc aatgcccgtt ccagttcgac cacctgccgg gcgtaggcgt
     1741 ccaagccccc ggcctgcgag cgccagccgc gtttttccct ctccttcttg gagatgagac
     1801 gatctactcc agtctcgttg atgaacacgc gcagccgtga ggtgtccacg cccatggcga
     1861 tccactgctc tacgttccac tgcacacggc ccagaccgtg ccagcggtca agcgggccga
     1921 tattctccga tggtaaccag taagcgtgca ggccgacata gtgccccttg cgcgcggcat
     1981 aatccacggc ctcggcgagt tgctccaact tccagcgctc taccgcacga cactcggcat
     2041 cgctccggcc cctttccccc agcccattgt cgccaggatt gccctccggc aggttgagga
     2101 tgcacaggcg caagccgttc gcctcggcct ctttcatcgc gccgagggag taaaggcgca
     2161 ggttggctag cccctcattg gagttacagt ccggctcgtt ggccgtctca aacaccacct
     2221 cgccccaacc aagaaactgg cgcatacgcg ggaggaagtc acgcacccac tggcgcccgc
     2281 cttcctctcc cctggcgata taggagg
//
LOCUS       DAOSCV010000751         2306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30526_length_2306_cov_3.706353, whole genome shotgun sequence.
ACCESSION   DAOSCV010000751 DAOSCV010000000
VERSION     DAOSCV010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30526_length_2306_cov_3.706353"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLY56_15795"
     CDS             complement(<1..211)
                     /locus_tag="PLY56_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOJ22990.1"
                     /translation="MPRIDGRAPDQMRPVKFTRHYNKYAEGSCLIEVGDTRVICTASI
                     EERVPLFLRNTGSGWINAGTVSDPSA"
     gene            complement(198..1022)
                     /gene="murI"
                     /locus_tag="PLY56_15800"
     CDS             complement(198..1022)
                     /gene="murI"
                     /locus_tag="PLY56_15800"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOJ22991.1"
                     /translation="MNPADPDDGPIGLFDSGVGGLTVVAHLLQRLPEESVLYFGDTAH
                     LPYGPRPLEEVRDYAIRIGRYLAAQGAKIVVMACNTSTSAAFDAVQEDLPVPVLGMVE
                     YGVHAALAVARNGKVGVIATQGTVLSGAYERAFRAVREDLQVVQQACPEFVPLVESGA
                     TDSPEALAAIQQYIAPLSAQGIDTLVLGCTHYPYLAGTIQQVVGPNVVLVDPAEALVQ
                     ALERLLDEKGLRRRSPDPPTHRFLVSGDPAAFVAAAHRLLHREILVEKVDIFDAEN"
     gene            complement(1019..1609)
                     /locus_tag="PLY56_15805"
     CDS             complement(1019..1609)
                     /locus_tag="PLY56_15805"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ22992.1"
                     /translation="MRRTTLIALLALIAAAGALLWWSRTSTPPMEPPRAETPPANAPE
                     GPSQEVTLYFAREVSSAEFVGWVLVPVQRPVAPSASPEQAVLEALAAGPTSEERQAGL
                     VATLPNGTRVLGVQREDGVITANFSRELQENFNGGAEWEEVVLYSIVNTLVEAGNAGS
                     VRIRIEGEPVESIGGHLAADEPLQRNDDIVFHGNQR"
     gene            complement(1611..>2306)
                     /locus_tag="PLY56_15810"
     CDS             complement(1611..>2306)
                     /locus_tag="PLY56_15810"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ22993.1"
                     /translation="TLVLILRSALGLEPVRGIQGLKVMLDPGHGGSLHGTTGLSGRRE
                     QDINLDVALRLYKMLEQAKARPMMTRFGDDTVSLHARPAMANAKGAHIFISIHANSNG
                     RPNSARGIETYYGHDRSYRLARTLHQQLVKELGAPDRRVRKRPGLVVTRETKMPSVLL
                     EIGYMNHREEDRLLGLPEYRQRVAKAIFNGIVKYMGGEAALDSRNAGSPQAEESEVGA
                     IELTVSLAGEEQH"
BASE COUNT          385 a          783 c          726 g          412 t
ORIGIN      
        1 cggccgaggg gtcgctgacg gtgccggcat tgatccagcc gctgccggtg ttgcgcagga
       61 agaggggaac acgctcttca atcgaagccg tgcagatcac tcgcgtgtcg cccacctcga
      121 tcaggcagga accctcggcg tacttattgt agtggcgcgt gaactttact gggcgcatct
      181 ggtcgggcgc ccgcccgtca attctcggca tcgaatatat ccaccttctc cacgaggatc
      241 tcgcggtgca acaatcggtg tgcggcagcc acgaacgcag ccggatcgcc actcaccaag
      301 aaccgatgtg tcggcgggtc gggggagcgg cggcgcagcc ccttctcgtc cagaagccgt
      361 tccagcgcct ggactaacgc ctcggccggg tcgaccagca ccacattcgg gcccaccacc
      421 tgctggatcg tgcccgcgag gtagggataa tgcgtgcagc ccaggacgag cgtatcgatc
      481 ccctgggcgc tcaacggcgc gatgtactgc tggatcgcgg ccaacgcctc gggactatcg
      541 gtcgctccac tctcgacgag cggcacgaac tcggggcacg cctgttgcac cacctgaaga
      601 tcctcccgga ccgcccggaa ggcacgttcg taagcgcccg agagaacggt tccctgggtg
      661 gcaatcacgc ccactttgcc gttacgcgcc accgccagcg cggcatgcac tccatattcc
      721 accatcccga gcacgggtac gggaaggtcc tcctggacgg cgtcgaatgc tgccgacgtg
      781 gaggtgttgc acgccatcac cacgatcttg gcgccctggg cggccagata ccgcccaatg
      841 cggatggcat agtcgcgcac ttcctccagg gggcgtggcc catacggcag atgcgccgtg
      901 tcgccgaagt acagcaccga ctcctcgggg agcctctgca ggaggtgtgc caccacggtc
      961 agcccgccga ccccagagtc gaacaggcca ataggaccgt cgtcaggatc tgccgggttc
     1021 accgctggtt cccgtggaat acgatgtcgt cgttgcgctg gagcggctca tcggcggcca
     1081 ggtgccctcc gatgctctcc accggctcgc cctcaatccg gatgcgcacc gagcccgcgt
     1141 tccccgcctc caccaaagta ttcacgatgg agtagagaac aacctcttcc cactcggcgc
     1201 ctccgttgaa attctcttgc agttcccggc tgaagttcgc ggtgatcacg ccgtcctccc
     1261 gctggacgcc caatacccgc gtgccattcg gcaaggtggc aacgaggccg gcctgtcgct
     1321 cctcagacgt cggacccgcg gccaacgcct cgagaacggc ctgctctgga gacgccgatg
     1381 gcgcgaccgg acgttgcacg ggcaccaaga cccaccccac gaactccgcg ctcgatacct
     1441 cgcgtgcgaa gtagagcgtg acctcctgcg aaggcccttc gggcgcattc gcgggcggcg
     1501 tctccgcccg gggcggttcc atcggtggcg tgctggtccg actccaccac aggagcgcgc
     1561 ccgcggcggc aatgagcgca aggagagcaa tcagtgtggt gcggcgcatc tcagtgctgc
     1621 tcctctccag cgagcgatac ggttagctca atcgcgccga cttcgctctc ctccgcctgc
     1681 gggctccctg cgttgcggct atcgagcgcc gcctcaccgc ccatgtactt gacaatgccg
     1741 ttgaagatgg ccttggccac ccgctgccgg tattcgggca atcccagcaa tcgatcctct
     1801 tcgcgatggt tcatataccc aatttccagg aggacggaag gcattttcgt ctcgcgggtg
     1861 acgacgagtc ccgggcgctt gcgtacccgg cggtcaggcg cgcccagttc cttgacgagc
     1921 tgttggtgga gggtgcgcgc gagccggtaa ctgcggtcgt gaccgtagta cgtctcgatg
     1981 ccgcgggcgc tgttgggccg tccattggag ttagcatgaa tgctgatgaa gatgtgcgct
     2041 cccttggcgt tggccattgc cggccgggcg tggagactga cggtgtcgtc tccgaagcgc
     2101 gtcatcatcg ggcgtgcctt ggcctgttcc agcatcttgt agaggcggag ggccacatcc
     2161 aggttgatat cctgctcgcg ccttcccgaa agcccggtgg tgccgtgcag gctgccgccg
     2221 tggcccggat ccagcatgac cttcagcccc tggatgccgc ggaccggctc cagaccgagg
     2281 gcgctgcgca ggatgagcac cagggt
//
LOCUS       DAOSCV010000752         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30561_length_2304_cov_4.481992, whole genome shotgun sequence.
ACCESSION   DAOSCV010000752 DAOSCV010000000
VERSION     DAOSCV010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30561_length_2304_cov_4.481992"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLY56_15815"
     CDS             <1..391
                     /locus_tag="PLY56_15815"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ22994.1"
                     /translation="VATFPWKPVEASFIGWLNKRLIPVLLKEAGICDLQRPCGELSGK
                     EIQRVAALLHLWRIPCAGVQSWMQSQVTAGGVSTREIDPETLESKRAPGVFFAGEVLD
                     IDGDSGGYNLQWAWSSGHVAGRLAAVA"
     gene            670..>2304
                     /locus_tag="PLY56_15820"
     CDS             670..>2304
                     /locus_tag="PLY56_15820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ22995.1"
                     /translation="MATEEQTPLDREIELSRDVGPQPESQPVAKQQGAEHGSASSEEC
                     YRSLVELSPTMIAVHDSRFLYINQAGVHLLGAANADEIVGTPALESIHPEDREVARAY
                     AEQALREGKPTPWVEQKLLRRDGSVVEVEALWTPILHEGRQELVVHVREITARKEIER
                     AMQRQQRLLDAIFDNILAHVAYMDRDFNIVRVNATYAQGSGHTVEELIGRNHFVLFPN
                     EDNQRIFERVRDTGQEYIARAKPFIYADQPERGVTYWDWWLRPIAGPMGEIEGLVLSL
                     VDVTSAERGRREVERLNAELQEVNATKDQFLAVLSHELRNPLAPILAGVLTLRQRMPD
                     DERVRRTIQLIERNVKLQTRLVDDLLDLSRIARGQLQFHHAHVLLDRVVKAAVEDQQG
                     EAQKAGLSMKVDTTPGLTVIGDFGRLQQAVLNLLTNAIKFSQRGGHIHVMTHAPDAQH
                     ARIVVEDTGIGIAPALIDRIFEMFRQGAAVAGTSAREWGLASRSSNRSSSSTMVACGP
                     RARAPARAAASPSNCRSYRIPHNQKLLREGRPRTCPQ"
BASE COUNT          449 a          726 c          729 g          400 t
ORIGIN      
        1 cgtcgcgacg tttccctgga aaccggtgga ggccagcttt atcggatggc tgaacaaacg
       61 gctgatcccg gtgcttctga aggaggcagg gatctgcgat cttcaacgcc cgtgcggaga
      121 attgagcggc aaggagatcc agcgggtcgc ggcgcttctg cacctgtgga ggattccctg
      181 cgccggcgtg cagtcgtgga tgcagtcgca ggtcaccgcg ggtggcgtct ccacccgcga
      241 gatcgaccct gagacgctgg aatcgaaacg ggcgcccggc gtgttctttg ccggtgaagt
      301 gctagatatt gacggcgaca gcggcggcta caacctccag tgggcatggt cctctggcca
      361 tgtagcaggc cggctcgccg cggtagcgtg acaaaccttg acggagaagg tctggaatct
      421 ggtataatgg cagcgtggaa ccgtcaggag agaatggccc tgttgttcgc gcctgggcag
      481 cgccggcggc ccatccctgg gcgtttcctc aaagttgaag tggcatccct gaccaccccg
      541 aattctttct cttatggacc atcgcgctgg cttaatactg ctccgcactc cgtagtgcct
      601 gtttgagcac cgccccccat gggcaaggca aaactcggag ttgctctgcc accgacatgg
      661 atgaggccta tggcaacaga ggaacagacg ccactggatc gggagatcga actgtctcgt
      721 gatgtcggcc cacagcctga gagccagccg gtggcgaagc agcagggcgc cgaacatggg
      781 tccgcatcaa gcgaggaatg ctatcgcagc ctggtggaac tatcgccgac gatgatcgcc
      841 gtgcacgact cgcggtttct atacatcaac caggcaggcg tgcaccttct tggcgccgcc
      901 aacgcggatg agatcgtggg tacccccgct cttgagagca tccaccccga ggatcgggag
      961 gtggcccgag cttacgccga gcaagcgcta cgcgaaggca agcccacgcc ctgggtggag
     1021 cagaagctcc tccgccgtga tggatccgtt gtcgaagttg aggcgctctg gacgccgatt
     1081 ctccatgagg gccggcaaga gctcgtggtg catgtgcgcg agatcaccgc tcgcaaggag
     1141 atcgagcgag caatgcaacg ccagcagcga ctgttggacg ccatcttcga taacattctc
     1201 gcccacgtcg cctatatgga ccgtgacttc aacatcgtcc gagtcaatgc cacctacgcc
     1261 cagggaagcg gccacactgt tgaggagctc atcgggcgca accacttcgt cctcttcccc
     1321 aatgaggaca accaacgcat cttcgagcgg gtgcgcgata ccggccagga atatatcgcc
     1381 cgtgccaagc cgttcatata cgccgaccaa ccggaacgcg gcgtcaccta ttgggactgg
     1441 tggctccggc cgattgccgg gcccatggga gaaatcgagg ggttggtcct ctcgctcgtc
     1501 gatgtcacct ccgccgagag agggcgccgg gaggtggagc gactgaacgc ggagttgcag
     1561 gaggtcaatg ccaccaagga tcagtttctc gccgtgctct cgcacgagct gcgcaacccg
     1621 ctcgcgccca tcctggcggg cgtgctcact ctccgccagc gaatgccgga tgacgagcgc
     1681 gtccggcgca ccatccagct catcgagcgc aatgtgaagc tccagacgcg cctcgtagat
     1741 gacctcctgg atctctcgcg cattgcccgc ggacagctgc agttccacca cgcgcacgtt
     1801 ctcctggacc gcgtggtcaa ggccgccgtg gaggatcagc agggcgaggc gcagaaggcg
     1861 ggcctctcga tgaaggtgga caccacgccg gggctcacgg tgataggcga cttcggccgg
     1921 ctccagcagg ccgtgctcaa cctgctcacc aacgccatca agttcagcca gcgcggcggg
     1981 catatccatg tgatgaccca tgcccccgac gcgcagcatg cgcggatcgt agtcgaggac
     2041 accggcatcg gcatcgctcc cgccctcatt gaccgcatct tcgagatgtt ccgccagggc
     2101 gcggcggtgg cgggcaccag cgcacgggaa tggggattgg cctcgcgctc gtcaaatcga
     2161 tcatcgagca gcacaatggt cgcgtgtggg ccgagagcga gggccccggc aagggcagcc
     2221 gcttcaccat cgaattgccg ctcgtatcgg attcctcaca atcagaagct gctcagggag
     2281 gggagacccc gcacctgtcc acag
//
LOCUS       DAOSCV010000753         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30563_length_2304_cov_4.138728, whole genome shotgun sequence.
ACCESSION   DAOSCV010000753 DAOSCV010000000
VERSION     DAOSCV010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30563_length_2304_cov_4.138728"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PLY56_15825"
     CDS             <1..384
                     /locus_tag="PLY56_15825"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ22996.1"
                     /translation="LVKPFTMPEMLSRIRAVMRRVHPRPAREATIEAGDLRIDLDARL
                     VTLAGRVISLTPKEFDILLTLASRPNRVFTHEELAERAWGPNEYVSPRTLAVHVGAVR
                     RKLGGGAGRPSPILTVRGVGFRFVA"
     gene            676..2208
                     /locus_tag="PLY56_15830"
     CDS             676..2208
                     /locus_tag="PLY56_15830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ22997.1"
                     /translation="MIQTLDVIVWVADIRTLQVYCLSYGAERILGYPSNPQSMDQDFL
                     RHHIHPDDRERVLQEIQACAAASRPARLEYRGVTMDGQVVRLRNHLHVLADPAGHSRR
                     LVGSIIDLTGSEKPESTPPGEGLLHEVLLRCIMAPAAVLDGNLMVTHVNKAFAHRWGC
                     SAPDLLGRSSAQVFSPKICAALARVRETREECVVAAPPAPAEANAGDVGAPDLWRLVP
                     VAGEGEGLAGIVMILAQETVPSSQESGIAPVPLRDAGALRERFLATVAHELRNPLTAM
                     LNSLEVVRAAVPEHPHATRAMNILERSIRLQARLIEDLMHFSAAASGQLRLHRTTVDL
                     HAVVSSAVDAERANAENASLALYVELTPGLWVNGDADRLQQAAANLISNAIKYTAPGG
                     EVFVRLSRMEGEPRTQASDSAASPHSEQPPPGWACLVVEDTGMGISPELLPRLFRVFQ
                     RGEDAARVQPGLGVGLALVWSIVEMHGGRVWVDSEGLGKGSRFGIELPLIEAPLQPLT
                     GA"
BASE COUNT          370 a          711 c          781 g          442 t
ORIGIN      
        1 ctggtgaagc ctttcacgat gcccgagatg ctcagccgga tccgggcggt gatgcgccgg
       61 gttcacccgc ggccggctcg agaggccacc attgaagcgg gcgatctccg cattgacctc
      121 gatgcccgtc tggtcaccct ggctgggagg gtgatctcgc tcactccgaa agagtttgat
      181 atcctgctca cgctggcctc acgccctaac cgcgttttca cccacgagga gcttgccgag
      241 cgggcctggg gacccaacga atacgtcagc ccacgcactc tcgctgtcca tgtcggcgca
      301 gtacgccgga agctgggcgg gggagccggg cgcccgtctc ccatcctgac tgtgcgcggt
      361 gtgggttttc gtttcgtggc ctaacgcatt cagcggggcc ttctgcatgc acgcgcgcgt
      421 tcctgggggt gggctggcga cgtggcaaca ttctttacga ttcctttgca ttcccttcgg
      481 catgcctgaa tcttcgtgtg ctatactctg caccgacggg agggggtcag ctcccaataa
      541 cccactgcgc atatggcgac tggcaggcct gtgacgctga tggcgcttat catggcacct
      601 cttcatcttt ttcccttgct gcagggatca tcgcagctat cggacctccg ggccgcactc
      661 aaggccgcgg aggagatgat ccagacgctc gacgtgatcg tttgggtggc ggatatcaga
      721 acattgcagg tctactgcct cagctacggc gctgagcgca tccttggtta tccctccaat
      781 ccccaatcga tggatcagga cttcctgcgc caccatatcc atcctgatga tcgcgagcgg
      841 gttctgcagg agatccaggc gtgcgctgct gctagtcgcc cggcccggct ggagtaccgc
      901 ggcgtcacca tggatgggca ggtggtgcgc ctgcgcaacc acctgcacgt gcttgccgat
      961 cccgcgggcc attcacggcg gcttgtgggc agcatcatcg atctcacggg ttcggagaag
     1021 ccggaatcca ctccacccgg tgaggggctc cttcacgagg tgctgctgcg ttgcatcatg
     1081 gcgccggcgg cggtgctcga tggcaacctg atggtgaccc atgtcaataa ggcgtttgca
     1141 caccgctggg ggtgcagtgc cccggatctg ctggggcgga gcagcgctca ggttttctcg
     1201 cccaagatct gcgcggccct ggcgcgtgtc cgcgagaccc gcgaggagtg tgtcgtggcc
     1261 gcgccgccag cgcccgcgga ggccaatgcc ggcgacgtgg gggccccgga tctgtggcgt
     1321 ctggtgccgg ttgctggcga gggggaaggc ctcgcgggta ttgtgatgat tctggcccag
     1381 gagacggtgc cctcttccca ggagtcaggc atagccccgg tgccgctgcg cgatgccggc
     1441 gcgcttcggg agcgttttct ggctactgtc gcgcatgagc ttcgcaaccc gctgaccgcg
     1501 atgctcaact cgctggaagt ggtgcgagcc gcggtgcccg agcacccgca tgccacccgg
     1561 gcgatgaaca tcctggagcg gagcatccgc ttgcaggccc gcctgataga ggacctgatg
     1621 cacttctccg cggcggcaag tggccagctg cgcctgcatc ggacgacggt tgacctccat
     1681 gctgttgtct cgtcggctgt ggatgcggag cgcgcgaacg cggagaacgc ctcgctcgcg
     1741 ctgtatgtgg agctcacgcc tggactgtgg gtgaatgggg atgccgaccg gctgcagcag
     1801 gccgcggcca acctgatcag caacgccatc aagtacacgg cgcccggcgg agaggtgttc
     1861 gtccgcctga gccggatgga gggggagccg cgaacccagg cgtcggattc cgccgcgtcg
     1921 ccacattccg agcagcctcc acccggatgg gcctgcctgg tggtggaaga caccggaatg
     1981 gggatctccc cagagcttct gccccggctg ttccgtgtat tccagagagg agaggatgcc
     2041 gcccgcgtcc agcccggcct gggggttggt ctggcgctgg tgtggtcgat tgtcgagatg
     2101 cacggagggc gggtgtgggt ggatagcgag ggtctaggca aggggagccg cttcgggatc
     2161 gagctgccgc tcatagaagc accgcttcag ccattgacag gcgcttaggc ggttgctaca
     2221 atggacaaaa agcgacatca aaggagatca gtcgcttgtg aaggggcgcc ctgaaaggag
     2281 agttcccatg aggcggcaat ccgg
//
LOCUS       DAOSCV010000754         2303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30579_length_2303_cov_3.497776, whole genome shotgun sequence.
ACCESSION   DAOSCV010000754 DAOSCV010000000
VERSION     DAOSCV010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30579_length_2303_cov_3.497776"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1345
                     /locus_tag="PLY56_15835"
     CDS             350..1345
                     /locus_tag="PLY56_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ22998.1"
                     /translation="MARPVTLFTGQWADLPLETLAEKAHEWGYDGLELACSGDHFDVE
                     KALSDPGYCQSRHDILAKYDLKVWAISNHLVGQAVLDIPSERIKAIVPERVWGDGKPE
                     GVRERAAEEMKNTARAAKALGVNVVNGFTGSSIWHLLYSFPPVPPEAIEDGFKELAAR
                     WNPILDVFRECGVKFALEVHPTEIAYDIYTAQRALQALDNRPEFGFNFDPSHLHWQFV
                     DPVEFIRAFPDRIYHVHVKDAARTLDGRTSILASHLNFGDPRRGWDFRSPGHGGIDFE
                     EIIRALNQVGYRGPLSVEWEDCGMDREHGAREAAAFVRDIDFTPSQIQFDAAFTK"
     gene            complement(1432..1812)
                     /locus_tag="PLY56_15840"
     CDS             complement(1432..1812)
                     /locus_tag="PLY56_15840"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ22999.1"
                     /translation="MTTINRIILQASRRYKVDPALIRAIVRAESRFDNRTLSHAGAMG
                     LMQLMPETARNLGVTNPWDPTQNIMAGTRFLRWLLDEFGSTRLAVAAYNAGPGAVRRH
                     GGVPPYAETQKYVNKVLAYRARGG"
BASE COUNT          414 a          722 c          734 g          433 t
ORIGIN      
        1 gagatcgtcg acgcgcacgg cgggacggtg aaagcagaga gcgagcccga caaagggatg
       61 cgcgtgacga tctacctggc ggccggggct actttggagt tccgagataa ggacatggat
      121 ggagcgggcc gcccacacgc gcagggctga agctccgcgc gccgggttgc gcgtcacctt
      181 cgagaatcct gattccccat ctcagggggc tcctcctggc ccttgacgga cgcccgtctt
      241 tctgttatct attagtagcg gtttcgcggg caatgcccgg gccgctggct ggctgccgtc
      301 cgcttgacgc tgtaccctgt cagatcactt cacgaaggag gggaccaacg tggcacgacc
      361 ggtaacactc ttcacgggac aatgggccga ccttccgctg gagacgctgg cggagaaagc
      421 ccatgaatgg ggctatgatg gtttggagct cgcctgctca ggcgaccact ttgatgtgga
      481 gaaggcgctc tcggatcccg ggtattgcca gagccgtcac gatatcctgg ccaagtatga
      541 cctgaaggtg tgggcaatca gtaaccacct ggtaggccag gcggtgctgg atatccccag
      601 cgagcggatc aaagcgatcg tgccggagcg cgtgtggggc gacggcaagc cggagggagt
      661 gcgcgagcgc gcagccgagg agatgaagaa tacggcgcgt gccgccaagg cgctcggtgt
      721 caacgtcgtc aacggtttca ccggctcgtc catttggcac ctcctctact cgttcccgcc
      781 ggtcccgccg gaggcgatcg aggatggctt caaagagctg gccgcgcgct ggaaccccat
      841 cctcgacgtc ttccgagagt gcggcgtcaa gttcgccctg gaggtgcatc ccacggagat
      901 cgcctacgat atctacactg cccaacgcgc actgcaggcg ctggataacc ggcctgagtt
      961 cggcttcaac ttcgacccga gccacctcca ctggcagttc gtggatccgg tggagttcat
     1021 ccgcgccttc cccgaccgca tctaccatgt ccacgtgaag gatgccgcgc gcaccctcga
     1081 cggccgcacc agcatcctcg cctcgcatct caattttggc gatccgcgcc gcggctggga
     1141 cttccgctcg ccagggcatg gcggcatcga tttcgaggag atcatccgag ccctcaatca
     1201 ggtgggctac cggggccccc tctcggtaga gtgggaagac tgtggcatgg accgcgagca
     1261 tggcgctcgc gaggcagcgg cctttgtgag ggatatcgac ttcacgccct cacagatcca
     1321 gttcgacgcg gccttcacga agtagaggca cgcccttcgt gggaaaggcg cgcccatcgg
     1381 gcgcgccttt cctcgctctg ggcactctgc atgtatcgat cggtgcagca gttacccgcc
     1441 tctggcgcgg tacgcgagca ccttattcac gtacttctga gtctccgcgt agggaggcac
     1501 gccgccgtgg cgcctcacgg cgccggggcc cgcgttgtag gcggcgacag caagccgggt
     1561 gctcccaaac tcatcgagca gccagcgcag gaagcgcgtg cccgccatga tgttctgggt
     1621 cgggtcccag ggattggtaa cccccaggtt gcgtgccgtc tccggcatca actgcatcag
     1681 gcccattgct cccgcgtgcg agagagttcg gttgtcaaag cgcgactcgg cacgcacgat
     1741 ggcacggatc aacgcgggat ccaccttgta acggcgtgaa gcctggagga tgatccggtt
     1801 gatcgtcgtc atgctgacgg tgccgcgcga tacaagccgg tcgcgcttgc tcttcgtagc
     1861 cgctgcggca ggcgatccga tcgccgatga aatcgctcct gcaatcggac tgctgccggt
     1921 ggtctcactg ggaccgagtg ctccggctcc cagcaagccg atgctgagcg ccaaaggcgc
     1981 cacaacccgc ttgagccggt gcgaatggat ggataggaaa ctgcagatcc tatcagtggt
     2041 ccgaagccgc tggtgttcgc ttgcgataga ctcgctgtcg cgagtcttca tgggtgtcaa
     2101 cagacggttc ccccttctgg taggttatca atgcccgcgc cgagcccgcg caagcggcgc
     2161 ccggtgcgca acaaatggat tggggcgccc caattgcgcc ccgtgaaaaa aaggaccccc
     2221 tcaaaccaga gcggttccac gcatttgttt gtatttttct ctcagtgggt agactcgtct
     2281 gttgcccagg gcatcgcggt gtt
//
LOCUS       DAOSCV010000755         2301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30631_length_2301_cov_4.034728, whole genome shotgun sequence.
ACCESSION   DAOSCV010000755 DAOSCV010000000
VERSION     DAOSCV010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30631_length_2301_cov_4.034728"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLY56_15845"
     CDS             <1..397
                     /locus_tag="PLY56_15845"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ23000.1"
                     /translation="NPEKPVTVQYGANVAVFKTTPEKQRAAWSFVRWLAEPNQTAYWA
                     THSTYMPLRRSVADRPEMKAYWEKDPQAQQCFEVAQYGVREPNVRGWQDVRKFMEEAF
                     DKVVSGQKAPEAALAEAATKANAALKERQ"
     gene            complement(486..>2301)
                     /locus_tag="PLY56_15850"
     CDS             complement(486..>2301)
                     /locus_tag="PLY56_15850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ23001.1"
                     /translation="FESLHRRRDGSTFPVEISARGATIDGTRVLVSVIRDVTERKRAE
                     EALRESEARFRQLANAMPQLVWTARPDGTVDYYNERVREFRGFAQDADGNWLWQPVLH
                     PEDVQPTVDAWEHAVRTGTIYQIAHRVQRADGTYRWYLSRGVPARDEEGRIVRWFGTA
                     TDIDDQKRAEERVRFLGEASAILASSLDSEKTLQRVARLAVPALADWAVLDLIGEGGA
                     IRRAVVAHREGVSPEEVARFERLAPSPESPLPEAHAFRTGETVFVCDSHTLSPPSWQG
                     DEQNRALRALGVHSVIAAPVVAGGRVLGAITLMWAEPDRRPGPEERLLVEELGRRVGV
                     ALDYARLFQSEQRARAEAQEANQSKDQFVAMVSHELRNPLAAITAGMDLLRRSIPLEG
                     RIGRAIEIVGRNAELQVRLVNDLLDLSRLNRGKLQLQRTPVNLQRVVDAAVHSYEAEA
                     KDAGVALTSDAEASLWVDGDFDRLQQVVMNLIDNALKVTPAGGTVHVAAKAHNERHAR
                     ITVEDTGVGMSQETLAHLFTLAHQGESGQRKGGLGIGLTLVKGIVERHGGRVWAESEG
                     PNKGSRFTVELPLRESPQAPDLPPARLSSEMPASSEAE"
BASE COUNT          413 a          793 c          715 g          380 t
ORIGIN      
        1 aaaccccgag aagccggtca ccgtgcagta tggcgccaac gtcgccgtct tcaagacgac
       61 gcccgagaag cagcgcgccg cgtggtcctt cgtccgctgg ctcgcggagc cgaaccagac
      121 cgcgtattgg gccacgcact ccacctacat gccgctgcgc cgctcggtcg cggatcgccc
      181 tgagatgaag gcatactggg aaaaggatcc gcaggcgcag cagtgcttcg aggttgcgca
      241 gtatggcgtg cgcgagccaa acgttcgcgg ctggcaggat gtgcgcaagt tcatggagga
      301 ggcgttcgat aaggtcgtct ccgggcagaa ggcgcctgaa gccgccctcg ccgaggccgc
      361 gacgaaggca aacgccgcct tgaaggaaag gcaatagcga tccgcacgag gccaagtgtt
      421 cgcctggagc atcgctttga ggatttttag acgcgcggga tcgccctcct gatgggcgcc
      481 cgcgcctatt ccgcctcaga cgaagccggc atctcggaag agagccgggc gggaggcaga
      541 tcgggcgctt ggggcgattc gcgcaaagga agctccacgg tgaagcgact ccccttattg
      601 ggcccctcgc tctccgccca gacacgcccc ccgtggcgct ccacgatccc cttcaccaga
      661 gtgagcccga tacccagccc gcccttccgc tgaccgctct ctccctgatg agcgagcgtg
      721 aagaggtggg cgagcgtctc ctgactcatg cccacgccgg tatcttcgac ggtgatgcgc
      781 gcatgtcgct cattgtgcgc cttggcggcc acgtgaacgg tgccaccggc aggcgtcacc
      841 ttcagggcgt tgtcaatcag gttcatcacc acctgctgca gccggtcgaa atcgccatcc
      901 acccacaggc ttgcctcagc atcgctggtg agagcgacgc ccgcatcctt tgcttccgct
      961 tcataggaat gaaccgcggc atcaaccacc cgctgcaggt tcacgggggt acgctggagt
     1021 tgaagcttcc cgcgattcag ccgagagaga tcgaggaggt cattgaccag ccgtacctgc
     1081 agttcggcat tgcgcccaac gatctcgatc gctcgcccga tccgtccctc gagagggatg
     1141 ctacggcgca gcaggtccat ccccgcggtg atagcggcca gcggattgcg caattcgtgc
     1201 gacaccatcg ccacgaattg atccttggac tggtttgcct cctgggcctc ggcacgcgcc
     1261 ctctgctcgg attggaaaag gcgggcgtaa tcgagagcca cacccacgcg ccggcccagc
     1321 tcctctacca gcaaccgctc ctcgggaccg gggcggcgat ccggctctgc ccacatcaga
     1381 gtgatcgccc ctagaacgcg cccccccgcg accacgggcg ctgcgatcac cgaatgcacc
     1441 cccagcgccc gtaacgcgcg gttctgctca tcgccctgcc atgagggcgg cgaaagcgtg
     1501 tgagaatcgc acacgaagac ggtctctccg gtgcggaaag cgtgggcctc cggcagcggc
     1561 gattctggcg agggcgccaa ccgctcgaac cgggccactt cctcggggga gaccccctcg
     1621 cgatgcgcca ccacggcacg gcggatggcg cccccttccc cgatgaggtc caacacggcc
     1681 caatcagcga gcgcgggcac agccagcctc gccacgcgct gcaacgtctt ctcagagtcg
     1741 agcgacgagg cgaggattgc gctggcctct cccaagaagc ggactcgctc ctcggcacgc
     1801 ttctggtcat cgatatcggt cgccgtaccg aaccacctca cgatccggcc ttcctcgtcg
     1861 cgagcgggaa ccccacggct gaggtaccag cggtaggtgc catcggcccg ctggactcga
     1921 tgggcaatct gatagatggt cccggtgcgc accgcgtgct cccaggcatc aacggtcggt
     1981 tgcacatcct cgggatggag gacaggctgc cacaaccagt tgccgtcggc atcctgagcg
     2041 aagcctcgga attcgcgcac gcgctcgttg tagtagtcga cagtgccatc cgggcgagcg
     2101 gtccatacca gttgaggcat cgcgttggcc agctggcgga agcgtgcctc gctctcccga
     2161 agcgcttcct cagcacgctt gcgctcggtc acatcgcgga tcacgctcac cagcacgcgt
     2221 gtgccgtcga tggtggcgcc tctggcgctg atctccaccg gaaaggtgct gccatcgcga
     2281 cgccggtgaa gcgattcgaa g
//
LOCUS       DAOSCV010000756         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30661_length_2300_cov_2.772383, whole genome shotgun sequence.
ACCESSION   DAOSCV010000756 DAOSCV010000000
VERSION     DAOSCV010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30661_length_2300_cov_2.772383"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..2120)
                     /locus_tag="PLY56_15855"
     CDS             complement(189..2120)
                     /locus_tag="PLY56_15855"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23002.1"
                     /translation="MATYSALFSPGRIGSREAKNRLVMAPMVRNYADREGRVTPRFLA
                     HMERIARGGVGTMILEASYVTPEGKGFANQLGIHSDDLIPGLAELAQLAHAQGALIGP
                     QLFHAGRQTSAAVTGTQPVAPSAVTCATVGEEPRALSVEEIQTIVDAFGQAARRAKEA
                     GFDFVELHGAHGYLLMQFLSPLTNTRDDAYGGSREKRMRFLEEVVRATRDAVGAGFPI
                     IVRLSGDELLPNGLKMEDTIAIARRLEELGVDALHISAGGYDSYNTGLMIQPMAIPDG
                     PLIPYAQGVKQAVGIPVIAVGKLRSPELGDQLIRDGKADFVAIGRSLLADPEWPNKAR
                     EGRLKEITPCIACNQGCISRLFLQRDVWCTVNPVTARELEFSKPAPATKKRVLIAGGG
                     PAGMEAAKVATERGHSVVLFEEDDHVGGQLIAATAAPFRPGWAELDNYLIDEMVRLGI
                     DVRLNTKVTPDVVRQEKPDAVILATGSSAVTPDFPGVQLANVVIARDLLEGKATAKGR
                     VVVAGGGCAGAQTAEYLAERGHPVVIVEMLDEIAVDAPRADRELLLERLRKRGVEFRV
                     RTKNTEMKAGSVTVEDPQGTHEWPADTVVICLGSRANDELVRELQGVIGHVISAGDAV
                     KPRQVTEAIAEGALAALSL"
BASE COUNT          355 a          795 c          763 g          387 t
ORIGIN      
        1 cactggttgc cagcgcgtgc aggctggccc agcccgccag ggtttcgctt acacgcagcg
       61 cggtgatcta ccgccgcgcg ttccttggcc ggttttcgcc tgggcgtcac ccgcgaccgt
      121 cgcgcggtcg ctccccttcc atagggctgg cgcggagggc ggggccggta acgaccccgc
      181 ctgcccgatt agagcgagag cgcggcgagg gctccctccg cgatcgcttc tgtcacctgg
      241 cgcggcttga cggcgtcgcc cgcgctgatc acgtggccga tgacgccctg cagctcgcgg
      301 accagctcat cgttggcgcg cgagcccagg cagatcacca cggtgtctgc cggccattcg
      361 tgcgtgccct gcggatcctc gacggtcacc gatccggctt tcatctcggt gttcttcgtc
      421 ctcacccgga actcgacgcc gcgctttcgc aggcgctcga gcagcagctc ccggtcggcg
      481 cgcggcgcat cgacggcgat ctcgtcgagc atctcgacga tcaccacggg gtgcccgcgc
      541 tcggccaggt attcggccgt ttgcgccccg gcgcaaccgc cgccagccac gaccacgcgg
      601 cccttcgccg tggcttttcc ctccaacagg tcgcgggcaa tgacgacatt cgcgagctgc
      661 accccgggga agtccggtgt gacggcggag gagccggtgg cgaggatcac cgcgtccggt
      721 ttctcctggc gcacgacgtc gggcgtcacc ttcgtgttca aacggacatc gatgcccagg
      781 cggaccatct catcgatgag gtagttgtcc agctcggccc accctggccg gaagggcgcc
      841 gcggtggcgg cgatgagctg gcctcccacg tgatcatcct cttcgaacag cacgaccgaa
      901 tgcccgcgct ctgtcgcgac tttcgcggcc tccatgccgg ccgggccgcc gccggcgatc
      961 agaacgcgct ttttcgtggc gggcgccggt ttggaaaact cgagctcgcg cgcggtgacc
     1021 ggattgacgg tgcaccagac gtcccgctgg agaaagaggc gcgagatgca tccctggttg
     1081 caggcgatgc agggagtgat ctccttgagc cggccctcgc gcgccttgtt tggccactcc
     1141 gggtcggcaa gaagcgagcg cccgatagct acaaagtcgg ccttgccgtc gcgaatgagc
     1201 tggtcgccca gttcgggaga gcgcagcttt cccacagcga tcaccggaat gccgaccgcc
     1261 tgcttcacgc cttgcgcata gggaatcagt gggccatcgg ggatcgccat cggctgaatc
     1321 atcaggccgg tgttatacga gtcgtagccc cctgcagaga tgtgcagcgc gtcgactccc
     1381 aactcttcca ggcgccgggc aatggcgatg gtgtcctcca tcttcaggcc gttcggcagc
     1441 aactcatccc ccgaaaggcg cacgatgatc gggaagccgg caccgacggc gtcgcgcgtg
     1501 gcccgcacca cctcctccag gaagcgcatc cgcttctcgc gggagccgcc gtaggcatcg
     1561 tcgcgcgtgt tggtgagcgg cgagagaaac tgcatcagca ggtagccgtg ggcgccgtgg
     1621 agctcgacga agtcgaagcc ggcctccttg gcccggcgcg ccgcctgccc gaaggcgtcc
     1681 acgatcgtct ggatctcctc cacgctgagc gcgcgcggct cctcgccgac ggtggcgcag
     1741 gtcaccgctg agggggcaac cggctgcgtc ccggtcaccg cggcggaggt ctgccgtccg
     1801 gcatgaaaca gctggggacc gatcagcgcc ccctgggcat gtgcgagctg cgctagctcc
     1861 gccaatccgg ggatcaggtc atcggagtga atgccaagct gattggcaaa gcctttccct
     1921 tcgggagtga cgtaagacgc ctccaggatc atcgtgccca cgccgccgcg ggcgatccgc
     1981 tccatgtgcg cgaggaacct cggggtgacc cggccttcgc gatccgcgta gttgcgcacc
     2041 atcggcgcca tgaccaaccg attcttcgcc tcgcgcgagc cgatgcggcc cggcgagaac
     2101 agggctgagt aagtggccat ctctcctcct ggtgccagtg ggccgcgcgg gcggggaacg
     2161 gcccagtgat gggggtgtcg gtggtggccc atcccagggc gtgttaagac accctctcga
     2221 gaatacttat tgcccgtaga gccagggcct aaccctcgga ggagcgataa caccgcgcgg
     2281 gaccacggat tcgctgacaa
//
LOCUS       DAOSCV010000757         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30671_length_2299_cov_3.329323, whole genome shotgun sequence.
ACCESSION   DAOSCV010000757 DAOSCV010000000
VERSION     DAOSCV010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30671_length_2299_cov_3.329323"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="PLY56_15860"
     CDS             <1..1666
                     /locus_tag="PLY56_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOJ23003.1"
                     /translation="RVRDFALPKRPILQSMIGLSDGNIFRAHGAKTPEEKEKIIRLYF
                     EEYIRARLSPFLYAPGTMAFNPLPDGAIRWEFVKDAQGQPTGEVKLDFTNFDREARRY
                     FDERQVFSAFNFAPYLLRHKTVDGKREAYLAFTDSNRVTIDGLLPDGTLNPVFEKAVV
                     SFFRQVGAHLAEKGWLDRAVYYVTDEPPDSDTDLIKQVCLLVRRGDPRIRTALTYDPA
                     NRPRLAEVQENGRSLISLWIPYVSFYREEVAAAERAKGAEYWLYDVKETCLISHSGER
                     NRGMFWDVWRRDAHGYLYYLATYWGRDATPWDRPSFLLPGVTYQYRHGDGYFFYPPRR
                     QYDPDPPILDTVVRSIRWELMREGAEDFDTLRILERLTDQAEKRRLPAAAQGRKALAL
                     ARSAAEAVTTGADAYGIRDLRFDAQPGWSFSAQEGWLHHRGGSRSDLVIHLDKVREDG
                     VYELILNVYDDLNYRGQPYSRFLVNGKAYATPGGLKGATDVAAGTLEVRGGRATFTLS
                     ALDEQAGVILYRVGLKRPSKPGTSLYAARTAVFDAIEALAAALKQR"
     gene            complement(1760..2002)
                     /locus_tag="PLY56_15865"
     CDS             complement(1760..2002)
                     /locus_tag="PLY56_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23004.1"
                     /translation="MPREEQGRLVRYHLYIDDGGNGHLIPEGGPLPSEARRAPRWRGT
                     TVIVEAAANTPEDQMQLALQSALAEWHRIEEARKRR"
BASE COUNT          384 a          784 c          730 g          401 t
ORIGIN      
        1 ccgggtgcgc gattttgccc tcccgaagcg cccgatcctc cagagcatga tcggcctctc
       61 ggatggcaac atctttcggg cgcatggcgc gaagacgccc gaggagaagg agaagatcat
      121 ccggctctac ttcgaggagt atatccgcgc gcgcctcagc cccttcctct acgcccctgg
      181 cacgatggcg ttcaatccac tcccggacgg cgccatccga tgggagttcg tcaaggacgc
      241 gcagggccag cccaccggcg aggtgaagct cgacttcacc aacttcgacc gtgaggcccg
      301 gcgctatttt gacgagcgcc aggtgttcag cgcgttcaac ttcgcgccct acctcctccg
      361 ccacaagacg gtcgacggga agagggaggc gtatctcgcc ttcaccgata gcaaccgggt
      421 gaccatcgat ggcttgctgc cagatggcac cctcaacccg gtgttcgaga aggccgtcgt
      481 cagcttcttc cgtcaggtgg gcgcgcacct ggcagagaag gggtggctgg atcgcgcggt
      541 ctactacgtt acggacgagc cgccggattc cgacacggat ctgatcaagc aggtctgcct
      601 tctggtgcgc cgcggtgacc cgcgcatccg cacggcgctc acgtacgacc cggccaaccg
      661 gccccgcctg gcggaggttc aggagaatgg ccggtcgctg atctccctct ggatccccta
      721 tgttagcttc taccgcgaag aggtggccgc cgccgagcga gcgaaaggcg cggaatactg
      781 gctgtatgat gtcaaggaga cctgcctgat ctcgcacagc ggcgagcgaa atcgcggcat
      841 gttctgggat gtttggcgcc gcgatgccca cggctacctt tactatctgg cgacctactg
      901 gggccgggat gccacgccct gggaccgccc tagcttcctg ctgccaggcg tcacctacca
      961 ataccgacat ggcgacggct acttcttcta cccgccgcgc cgccagtatg atcccgaccc
     1021 gcccatcctc gataccgtcg tccgctccat ccgctgggag ctaatgcgcg agggcgccga
     1081 ggacttcgac acgctgcgca ttctggaacg cctaaccgat caggcagaga aacgccgcct
     1141 gcctgccgcc gctcagggcc ggaaggcgtt ggcgctcgcg cgctccgcgg cggaggcagt
     1201 caccacgggt gcggatgcct acgggattcg cgacctgcgc ttcgacgcgc aacccggatg
     1261 gtcgttcagc gcccaggagg ggtggctgca ccaccgcggc gggagccggt ccgacctggt
     1321 gattcacctg gacaaggtgc gcgaggatgg agtctacgag ctgatcctga acgtctatga
     1381 tgacctgaac taccgcggcc agccctactc gcgcttcctg gtgaacggga aggcatatgc
     1441 gacccccggc ggattgaagg gcgcgacgga tgtcgccgcc ggcacgctgg aagtgagggg
     1501 gggccgcgcc acgttcaccc tctcggcgct cgatgagcag gcgggcgtga tcctctaccg
     1561 ggttggactg aagcgcccgt ccaagcctgg cacgagcctc tacgcagcac gaaccgccgt
     1621 tttcgatgcc attgaggcac ttgccgcggc gttgaagcag cggtgaaaga ctgcgcgcgc
     1681 gtgcgctcct gatgcgtcct ggtcacggcg cttcgcggtg cccggtactc ggtcgcaggg
     1741 gcgcacgcgt gcgcgctcct taccggcgct ttcgggcttc ctcgattcga tgccactcgg
     1801 cgagagcgct ctggagcgcc agctgcatct gatcctcggg cgtattggcc gctgcctcca
     1861 cgatgaccgt cgtgccccgc cagcgcgggg cacgtcgggc ttccgatggt agcggccctc
     1921 cctcggggat cagatggccg ttgccgccat cgtcgatgta gagatgatac cgcacgaggc
     1981 gcccttgctc ttccctaggc atgccgctct cctcccatcc accatgctcg cggagtgaat
     2041 atacccggcg gggtacgggc acactccggc cgagaagcaa ccacggctcc cctcaccttc
     2101 ttcgcggtca ccgccggcgg ggcctcttcg gcgccatcgg atccgagaaa aagccggcgg
     2161 gcttaccacg gctggtttgc ggcgctccag tgaatgagcg gcatccatcg cgtgtggcca
     2221 tcgaggtaga ggacattgcc accctcccgg tgagcccctg atggaacgcc gccgttgtag
     2281 tcccacaaca ccggaaccc
//
LOCUS       DAOSCV010000758         2292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30795_length_2292_cov_3.553420, whole genome shotgun sequence.
ACCESSION   DAOSCV010000758 DAOSCV010000000
VERSION     DAOSCV010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30795_length_2292_cov_3.553420"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLY56_15870"
     CDS             complement(<1..438)
                     /locus_tag="PLY56_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23005.1"
                     /translation="MAERNYQFRQRLNIVHQPGRRDPDLRPEQGETVIEEGWRIAVAP
                     DASEYLVGVAKDFQDYLFTSMGISTLLVREAPAEKSRTIRLTTKAESPELGKELSVAR
                     SYRVLVEPDSITVCGCDERGTGQGCYYLEDLMNLREAPFLAKMD"
     gene            878..1300
                     /locus_tag="PLY56_15875"
     CDS             878..1300
                     /locus_tag="PLY56_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016194544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ23006.1"
                     /translation="MSVNWVGYALLSAVFAGLTALLAKMGVANVPSNLAMFIRTMVVV
                     LFAGGIAIATGDVGYFGKLSSRNWIFLVLSGIATGLSWIFYFAALKYGPVSRVAPIDK
                     LSFVLAMALGVFVLGESVKPVTLAGALLIVAGVLLTLR"
     gene            1445..2140
                     /locus_tag="PLY56_15880"
     CDS             1445..2140
                     /locus_tag="PLY56_15880"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ23007.1"
                     /translation="MKSYQWAVLGALAILMASVPAAATEKMIVVDSETALVYPLEDGR
                     LVREPIISNNRKEFVWKDIGTFRITEKIIDKRSNLYNTDGDPIRPGEKGAQMRYWMRM
                     GWTAQGFHQSALFSPELPRRRSHGCYRLSKSSAEWLFNWTPVGTPVHVVRRLKDSRFA
                     GLAKQQPITMAQRGSGANATPRTPAKPAQAAAAAKTSGRAVTKAAVTVKPAPRPVATT
                     ARVTIKRVDSRSS"
BASE COUNT          400 a          696 c          760 g          436 t
ORIGIN      
        1 gtccatcttc gcgaggaagg gagcctcgcg caggttcatc aggtcttcaa ggtagtagca
       61 cccctggccc gttccgcgct cgtcgcaccc gcacaccgtg atggaatcgg gctcgaccag
      121 gacgcggtaa ctgcgcgcga ccgacagctc cttgccgagt tcaggagact ccgccttcgt
      181 ggtgaggcgg atcgtgcggc tcttctcggc gggtgcctca cgaaccagga gcgtggagat
      241 ccccatgctc gtgaaaagat agtcctggaa atctttcgcc acgccaacga ggtactccga
      301 ggcgtcaggt gcaacggcga tgcgccagcc ttcctcgatg acggtttcgc cctgctccgg
      361 gcgcagatcc gggtcgcgtc tacccggctg atggacgata ttcaaccttt gtctgaactg
      421 gtagttgcgc tctgccatct gctctcctca caaaggccag tcgacggtgc ggcggggtgc
      481 ggccggatca ggccgttcct tcccgcccat ccgtatggct ggcagcggaa ctccgggcaa
      541 ggcccttttc cggcaggccg gcctctccgc tctggcagtg agttcggctg ccgacgccgg
      601 gttcctgcca ggaccggcgc gcgagcggcc acacgggcgt cttccgaccg agagaatgtg
      661 cccaccgcgt cgcaggagtt gagggaggga ggcacgaacg ccctggcagc gaaaccagag
      721 agaggcccgc tggctttgga acagcgcggc gagagcgcgc acgttccagt gccatgccag
      781 gctgggcatt tcgtcccgcg tggcgaggtg gcgcctgtcg cggggcctgg ggagtgctgt
      841 ccgctgcctg gcaggcgcgc ccgcggggag agggaggatg tcggtgaact gggttggata
      901 cgcgttgctc tcggcggtct tcgcgggttt gacggcgttg ctggcgaaga tgggcgtggc
      961 caatgtgccc tctaacctgg cgatgttcat ccgtacgatg gtggttgtgc tcttcgccgg
     1021 cgggattgcc atcgccaccg gcgatgtcgg atacttcggc aagctctcct cgcgcaactg
     1081 gatattcctg gtgctctcag gcattgccac cgggctctcc tggatcttct acttcgcagc
     1141 cctcaagtac gggccggtct cccgcgtcgc gccgattgat aagctcagct tcgtcctggc
     1201 gatggccctg ggggtgttcg tcctcggtga atcggtcaag ccggtgacgc tcgcaggcgc
     1261 gctgctcatc gttgccggag tgctcctcac cctgcgatag ccagccgcgc cggcccggcg
     1321 atccgccacg ccgactcgag agaatcggaa tgggaaccgt gccccggcat catggtcctt
     1381 acccaaaaga atgttgtata gtgtgacaca gggaatctgt agacatgaat gcggaaggga
     1441 tctggtgaag agctatcaat gggcggtgct gggcgcgttg gcgattctga tggcgagcgt
     1501 gccagccgct gctaccgaga agatgattgt agtggactcg gagacggccc ttgtctatcc
     1561 actcgaggat gggcgtctgg tacgcgaacc gatcatttcg aataaccgca aggagtttgt
     1621 ctggaaggat atcggcacct tccggatcac tgagaagatt attgataaac gctcaaatct
     1681 ttataacacc gatggcgatc cgatccgtcc gggcgagaag ggagcgcaga tgcgctactg
     1741 gatgcggatg ggatggaccg cgcaggggtt ccatcaatcg gcgctcttct cgcccgagct
     1801 gccgcggcgg cggtcccacg ggtgttaccg cctctccaag agcagcgcgg agtggctttt
     1861 caactggacc ccggtaggca cgccggtcca tgtggtgcgc cgtctgaagg attcgcgctt
     1921 cgccgggctg gccaagcagc agccgatcac gatggcgcag cgcggcagtg gtgccaacgc
     1981 cacgccgcgg actcccgcca agcccgcgca ggcggcagcc gcagcgaaaa cgtcggggcg
     2041 tgccgtaacg aaggcggccg tgacggtcaa accggcacca cgcccggttg cgacgaccgc
     2101 gcgcgtgacg atcaagcgcg tcgattctcg cagctcctga tacagcgctc cggcgccacg
     2161 gggttgcgaa aagtgcctca ctgatctgaa aacagccatg cctaagggag ggggatggct
     2221 gttttcctct gtaccattta cagaatcctt gggcgatgaa agcccacacc ctttaggggt
     2281 gggatgaaat cg
//
LOCUS       DAOSCV010000759         2282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_30972_length_2282_cov_2.969915, whole genome shotgun sequence.
ACCESSION   DAOSCV010000759 DAOSCV010000000
VERSION     DAOSCV010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30972_length_2282_cov_2.969915"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..1281)
                     /locus_tag="PLY56_15885"
     CDS             complement(283..1281)
                     /locus_tag="PLY56_15885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOJ23008.1"
                     /translation="MAGNFKVWVTRQIPQQGIDMLREVADVEVWEDELPPPRDLILDK
                     IGELDGILALLTDKMDAEVMDRGKKLKVIANYAVGFDNVDVAAATERGILVTNTPGVL
                     TETTADLAFTLLMATARNIVVADKFTRDGKWKTWGPTLLLGQDIHHAKLGLIGLGRIG
                     REMAKRARGFDIDVTYYDVVRNEQAERELGLKYADIDTILKESDFVSLHTPLTPQTRH
                     LIGARELSLMKPTAILINTSRGPVVDQKALYEALRDKVIYAAGLDVFDVEPISKDDPL
                     LTLDNVTVVPHIASASFATRTRMATMAAENLIAGLKGTTPTAPVNPEVLNSPQRRK"
     gene            1748..>2282
                     /locus_tag="PLY56_15890"
     CDS             1748..>2282
                     /locus_tag="PLY56_15890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ23009.1"
                     /translation="MIHPRAPAAVDESNAQGDVAGALTDDALVARARAGDRDAFALLV
                     TRYQDRVYSITRAFVREPDDALDLVQDTFVKAFQGLPRFRGGSSFYTWLYRIAVNNCK
                     DFLRRRAVRPSVSLEEELLQDAGFEPASHDPHADPAGMAERVELCAAVQAAVEGASDV
                     GNAVMAATFTTVAVFLPV"
BASE COUNT          395 a          703 c          760 g          424 t
ORIGIN      
        1 ggaggagcgc aggctgcgcg aggtagagga tgagatgggc gatgtctacc tcatcgccta
       61 tgagaagccc ctggttccag cgcctctcga tgaggagaag ttgcggcggc tgcaggatgt
      121 tgagagggag atgccggggc gcatcgtggt ggcctacaag aggacgtagc cgctctcgct
      181 ggccgatatt cgaagggccg caggtgaacg gaagtgggca gggagggctc ccttccattc
      241 ccgctcacct gcgtctattt gcagcgaact gagctcaccc gcttacttgc ggcgctgcgg
      301 cgagttgagc acctccgggt tgaccggcgc ggtgggcgtc gtgcccttca accccgcgat
      361 caggttctcg gcagccatgg tggccatgcg agtgcgcgtg gcgaagctag cgctcgcgat
      421 gtggggcacc accgtcacgt tgtcgagcgt gagcagcgga tcgtccttcg aaatcggctc
      481 cacatcgaag acgtccaggc cggccgcgta gatcacctta tcgcgaaggg cctcgtagag
      541 cgccttctgg tcgaccacag ggccgcgcga ggtgttgatc aggatcgccg tgggcttcat
      601 caaggaaagc tcgcgggcgc cgatcaggtg ccgggtctga ggcgtcagcg gcgtgtgcag
      661 cgagacgaag tccgactcct tcaggatcgt atcgatatcg gcgtacttca ggccgagctc
      721 gcgctccgct tgctcgttgc ggacgacgtc gtagtaggtg acgtcgatgt cgaagccacg
      781 ggcccgcttg gccatctcgc ggccgatgcg gccgagaccg atgagaccca gcttcgcgtg
      841 gtggatatcc tggcccagca gcagcgtggg gccccaggtc ttccacttgc catcgcgggt
      901 gaacttgtcg gccacgacga tgttgcgcgc ggtggccatc aggagcgtga acgccaggtc
      961 cgcggtcgtc tcggtcagca caccgggggt gttggtgacc agaatgccgc gctcggtagc
     1021 tgccgcgacg tcgacgttat cgaatccgac ggcgtagttg gcgatgacct tcagcttctt
     1081 gcctcggtcc atcacctcgg catccatctt gtcggtgagc aatgccagaa tgccatccag
     1141 ttcgccaatt ttgtcaagaa tcaggtcacg cggcgggggc agctcatcct cccacacctc
     1201 gacgtcggcc acctcgcgga gcatatcgat gccctgctga ggaatctgcc gggtgaccca
     1261 gactttgaag ttaccggcca tgcaaatcct cccaacgtgt gctacgaaac accctggcgc
     1321 gggatgaggt gtgaggagag agaaacgaga ccgtgctgat cgcatccccc gccgcgtcag
     1381 gagcaaatgc ccctggcgct ccctcacccc accacgaccc gcggcctggt gacaactggg
     1441 gccatgccgg gcccctcggg ttctcaacca atcgattcgg gacgaccggg caaactcctg
     1501 ccggccggcg acgacgaaac gagggcctca tccccatctc gcggtcagat cggcgggtgc
     1561 cagagcgcaa ccttcgcgcg cgagactcgt ccctaggtac aggagctcac cacggggaga
     1621 gcaaagccgc aggggaacgt tatctcggca gagaatgggg agtacatgta tgatgcgcca
     1681 ggatgtttcg gcggccattt cagcagatgg aaccgggctc ctcaacttga ggcggggagg
     1741 tgctggcgtg attcacccgc gtgccccagc ggcggtggat gagtccaatg cgcaaggcga
     1801 tgttgccggc gcgctcacgg atgacgcgct cgtcgcgcgc gcacgcgcgg gagatcgtga
     1861 tgccttcgcg cttctggtga cgcgctatca agaccgtgtc tactccatta cgcgggcttt
     1921 cgtgcgcgag ccggacgatg ccctcgattt ggtgcaggac accttcgtca aagcgttcca
     1981 ggggctgccg cgcttccggg gcggttcgag cttctacacg tggctctatc ggattgccgt
     2041 caacaactgc aaggattttc tgcgccggcg cgcggtgcgc ccttccgtct cccttgaaga
     2101 agaactactc caggacgccg ggttcgagcc ggcttcccat gacccccatg ccgatcccgc
     2161 gggaatggcg gagcgcgtgg agctatgcgc cgcggttcag gccgccgtgg agggggcgtc
     2221 cgatgtcggc aacgccgtta tggccgcgac tttcactacg gtggccgtgt ttttaccggt
     2281 gg
//
LOCUS       DAOSCV010000760         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31027_length_2279_cov_3.247302, whole genome shotgun sequence.
ACCESSION   DAOSCV010000760 DAOSCV010000000
VERSION     DAOSCV010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31027_length_2279_cov_3.247302"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLY56_15895"
     CDS             <1..1054
                     /locus_tag="PLY56_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23010.1"
                     /translation="AEAELKKIPEIKLLYSGANVRIWDGPLVWANKSYAPPYNNPDIA
                     PIANSGPNIPPGHPGTADHVHPIANPVYKHYVRKLGFPTADEAANEESGSNMGVAYFK
                     THNHNATGLRYLVRHTTTGVIRELNDPTKPYTVMKNNDWRLDGEFNPNKKDLENLGMT
                     DEEEFYGIRAYPGQYYFEQIDMTNQSRLFLRTFKDSERPPLLANGELETVVVNDVGNP
                     NPGESENQNVRFWIGTTGGKDPDTTFDVNTVMEYPFWASRFRVFIASTGTITVRGRNT
                     NPPPPFRVNMLCYNKDASGKGYGHIHFQSATYLFGSLIAWMVDAVGGCIVEKQQPELG
                     PSNPLAYNAVDWRELE"
     gene            1051..1485
                     /locus_tag="PLY56_15900"
     CDS             1051..1485
                     /locus_tag="PLY56_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ23011.1"
                     /translation="MRRESGFSYLELLIAALVLAIATVGALATWSYSSRAPANKRVTE
                     MGVYVAVQEIERLKARRYMGLEDTPAGSPLVTYYDRYGAPSASGTPQPQGYKVKAFIT
                     PAVDRDATPNTEDLREIRVEVWDNNETTRYEEARVLLAFGGS"
     gene            1487..2110
                     /locus_tag="PLY56_15905"
     CDS             1487..2110
                     /locus_tag="PLY56_15905"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ23012.1"
                     /translation="MKRYGRSWMPGGPFRPKVPAAIRQRCRGLSLVEMLVAALVLSFL
                     VASTAAVYQGGEKQQRTARAYSLAQTDIRRALQRITRTARHGYQVVPTSNWGTFAGQS
                     SGMHQLIVEVPEPGATSRAQVRFYIQDGNVYAQRQNVPSPGILLIEGVQALEFRYYQT
                     DPGTGARPEVPPDQATEVEITLTAVRGPAVTTVKAYVNLRNAIGASL"
BASE COUNT          485 a          720 c          721 g          353 t
ORIGIN      
        1 ggccgaggcg gagctgaaaa aaattcccga aatcaagctg ctctactccg gcgcgaacgt
       61 ccggatctgg gacggcccgt tggtgtgggc caacaagagc tacgcgccgc cctacaacaa
      121 tccggacatc gcgcccattg ccaactcggg cccgaacatc cccccggggc acccgggcac
      181 cgccgaccat gtgcatccta ttgcgaaccc cgtttacaag cactacgtgc gcaagctggg
      241 cttccccacg gctgatgagg ccgccaacga agagtcgggc tccaacatgg gcgtagccta
      301 cttcaagaca cacaaccaca acgccaccgg attgcgctac ctggtgaggc acaccaccac
      361 gggcgtcatc cgcgagctga acgacccaac gaagccctac acggtgatga agaacaacga
      421 ctggcgactg gacggcgagt tcaatcccaa caagaaggac ctggaaaacc ttgggatgac
      481 tgatgaagag gagttctatg ggatccgtgc ttacccaggg cagtactact tcgagcagat
      541 cgatatgacg aaccagagcc gcctcttcct gcgcaccttc aaggactcgg aacggccccc
      601 ccttctcgct aatggcgagc tggaaacggt cgtcgtgaac gacgtgggca atccaaaccc
      661 gggcgagagc gagaaccaaa acgtgcgctt ctggatcggg accacgggcg gcaaagaccc
      721 ggataccact ttcgatgtca acacggtgat ggaatacccc ttctgggcct cgcgcttccg
      781 ggtctttatc gccagcacgg ggaccatcac cgtgcgcggg cgcaacacga acccgcctcc
      841 ccccttccgg gtcaacatgc tgtgctacaa caaggacgcg agtggcaagg gctacggcca
      901 catccacttc cagagtgcca cctacctctt tggctcgctc atcgcctgga tggtggatgc
      961 cgtgggcgga tgcatcgtcg agaagcaaca gcccgaactg gggccatcca acccactcgc
     1021 ttacaacgca gtcgattgga gggaactgga gtgaggcgcg agagcggttt ctcttacctg
     1081 gagttgctga tcgcggccct cgtcctcgcg attgccacgg tcggcgccct ggctacgtgg
     1141 tcctattcgt cacgcgcgcc agcaaacaaa cgcgtgacgg agatgggggt ctacgtggca
     1201 gttcaagaga tcgagcggct caaggcccgg cgctacatgg gactggagga tacgcccgcc
     1261 ggatcaccgc tggtcaccta ttatgatcgc tatggcgcgc cctccgcgag tggaacccca
     1321 cagccccagg gctacaaggt caaggcgttc atcacccccg cggtggatcg ggatgcgacg
     1381 ccgaacacgg aggacctgcg cgagatccgc gtcgaggtgt gggacaacaa cgaaaccacg
     1441 cgctatgagg aggctcgggt gctcctcgcc ttcggaggat cgtgaaatga agcgatacgg
     1501 ccgctcatgg atgccgggag gcccgttccg gcccaaagtg ccggcggcga tccgtcagcg
     1561 gtgtcgcggc ctgagcctgg tcgagatgtt ggtcgcggcc ctggtgctct cgttcctggt
     1621 ggcaagcacc gccgctgttt accagggagg cgagaagcag cagcgaacag cgcgggccta
     1681 cagcctggct caaacggata tccgaagggc gctgcagcgg atcacgcgga cggcgcgcca
     1741 cggttaccag gtggtgccca ccagcaattg gggtaccttc gcgggtcaat cgagcggcat
     1801 gcaccagctg atcgtggagg tgccggagcc aggggcaacc agccgggccc aggtgcgctt
     1861 ctacatccag gacggaaatg tctacgcgca gcgccagaac gtcccctcgc ccggcatcct
     1921 gctgatcgag ggagtgcagg cgctggagtt ccgctactac cagacagatc cgggcacggg
     1981 cgcccggccc gaggtgccac cggaccaggc gacagaggtg gagatcacac tcaccgcggt
     2041 aagagggccc gccgtcacga cggtcaaggc ctatgtcaac ctgcgtaacg ccatcggcgc
     2101 gagtctctga gagaagctcg accccgcccg cgcgtcgatg cacggcatac gggatgggag
     2161 tgtgtagagg cagatggcca agaaaatgac gggaagctcg gtgggcatcg tggtgacgcc
     2221 ccgcgccatc gtggcggtgc aagcgagcgc gcacgagaac gagatcgagg tcaaggtgc
//
LOCUS       DAOSCV010000761         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31033_length_2279_cov_2.763040, whole genome shotgun sequence.
ACCESSION   DAOSCV010000761 DAOSCV010000000
VERSION     DAOSCV010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31033_length_2279_cov_2.763040"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PLY56_15910"
     CDS             complement(<1..600)
                     /locus_tag="PLY56_15910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOJ23013.1"
                     /translation="MSGTRATPRIIVFNMNFIGDVLFTTPALAALRKGYPEAEVTVVV
                     GRRAKDVLVANPDVDHLVEFPRTLSERWQLLRRFFRRFDLSLSLSSRNVERAAWGMAL
                     GCRQRWGFNRPETRLFLTGRIEERRDRHWAEDYLELACLAGGKRNGDLPRFYLTPEEK
                     AWAEELPARWGLRPGHLWIGVHVGASYPEKAWRPERFLEL"
     gene            complement(848..>2279)
                     /locus_tag="PLY56_15915"
     CDS             complement(848..>2279)
                     /locus_tag="PLY56_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ23014.1"
                     /translation="IEAHTAPGETVLDSFCGSGTTLVEALATGRRAIGVDLNPMAAFI
                     CRVTITPADTDALRQAFEHLRAAVADEINALYAGDGEGIRPAHWYPTTELPVSAFGIM
                     AKDAGMTVADLFTPPNLTAAALLWHGIQTIPCDPVTRDLLRFSFTAAVAQFTKMIRTK
                     KNGTGWWVNAYRIPRTYRENNVWRSFENKLWAVVRMKEETNALLGPVSLSMGDPPEPN
                     AACRLRIGSATDLSFLPEESVDYVFIDPPWAIKYFDLSVLWCSWMGFELPFDQEIFPW
                     QQRFRRDMTQALAELARVLRPGKVLTLNFATRYASVLNEWREAIEATGLEWLGVTPLQ
                     QDQLSFVQRQHETTLKGNYLLTLRKHGSPGARATPAEATPVTEHEIAAMIRALLEERG
                     AMTYDQLFQALYPALARRPLACDLYTVLATRFGYSITEGRPYEEGVWHLGEGNADPPE
                     ASNHRVAPRMRGPRREAQLTLPFNGG"
BASE COUNT          383 a          741 c          765 g          390 t
ORIGIN      
        1 cagttcgagg aaacgctccg ggcgccacgc cttctccggg taggatgcgc ccacgtgaac
       61 accaatccac aggtgcccag ggcgcaagcc ccagcgtgcc ggaagctcct cggcccacgc
      121 cttctcctcg ggcgtcaggt agaagcgcgg caggtcgccg tttctcttgc cgcccgccag
      181 gcacgcgagc tccagataat cttccgccca atgccggtct cgccgctcct cgattcgccc
      241 ggtcaggaag aggcgcgttt ccggacggtt gaaaccccac cgctggcgac atccgagcgc
      301 cattccccag gcggctcgct ccacgttgcg cgaggagagc gagagcgaga ggtcgaagcg
      361 gcggaagaac cggcgcagga gctgccaccg ctcggagagg gtgcgcggga actccacgag
      421 atggtccaca tccgggttgg cgacgagtac gtcctttgcc cggcggccga cgaccaccgt
      481 cacctcggct tctggatacc ccttccgcag cgccgcgagc gccggcgtgg taaagaggac
      541 atcaccgatg aaattcatat tgaagacgat gatgcgtggt gtcgcccgtg tgcccgacat
      601 cccttattcc tgttctccgg ttgcccgatg gaggcgccgc cgccagcgtg agcctatccc
      661 gcgcgccggc gggctgctcc tcgcagccaa ttccatcgcc tcttccatgt ggacaacctc
      721 atctatccag aagtttctcg gataggcgtt cagggaaggg gagtgagggg ccagcacagg
      781 cctccggtcg agcaggacag ggtcagattc tgctcgtggc ggcgcatctc cttcccgaag
      841 tgtggtctca gcccccgttg aaggggagcg tcagttgcgc ctctcgccgc gggccgcgca
      901 tgcgcggtgc cacccggtgg ttgctggcct caggtgggtc ggcattcccc tcgccgaggt
      961 gccacacccc ctcctcgtac ggccgcccct cggtgatgct gtagccgaag cgggtggcca
     1021 gcaccgtata gagatcgcac gccagcggcc ggcgcgcgag cgccggatag agcgcctgga
     1081 agagctgatc gtaggtcatc gcaccccgct cctccagaag agcgcggatc atcgcggcga
     1141 tctcgtgctc ggtgacggga gtagcctcgg ccggcgtggc gcgcgcgccc ggcgagccgt
     1201 gcttgcgcag ggtgaggagg tagtttccct tgagggtggt ctcgtgctgc cgctgcacga
     1261 acgagagctg gtcctgctgc agcggggtaa cgccgagcca ctccagcccg gttgcctcga
     1321 tcgcctcgcg ccactcgttg agcacgctcg catagcgcgt ggcgaagttg agcgtgagca
     1381 cctttcctgg tcgcaacacg cgcgcaagct ccgcgagcgc ctgggtcata tccctccgga
     1441 aacgctgttg ccaggggaag atctcctgat cgaaagggag ttcgaagccc atccacgagc
     1501 accacaggac gctgagatcg aagtacttga tcgcccacgg cgggtcgatg aagacgtaat
     1561 cgacgctctc ttcagggagg aaagagagat cggtggccga gccgatacga agcctgcaag
     1621 cggcgtttgg ttcgggcggg tctcccatgg agaggctcac ggggcccaga agcgcgttcg
     1681 tctcctcctt catgcgcacc actgcccaca atttgttctc gaaggagcgc cacacgttgt
     1741 tctcgcgata ggtgcgcggg atgcgatagg cgttgaccca ccatccggtg ccgttcttct
     1801 tggtacggat catcttggtg aactgcgcga ctgccgcggt gaaggagaag cggaggagat
     1861 cgcgcgtgac cgggtcgcag gggatcgtct ggatgccatg ccacagcagc gccgcggccg
     1921 tcaggttggg cggggtgaag aggtcggcca cggtcatgcc ggcatccttg gccatgatgc
     1981 cgaaagcgga gacgggcagc tcggtggtcg ggtaccagtg cgcgggccgg atcccctcgc
     2041 cgtcgccggc atagagggcg ttgatctcat ccgcgaccgc ggctcgcagg tgctcgaacg
     2101 cctggcgaag cgcgtcggta tcggcaggcg tgatcgtgac gcggcagata aacgccgcca
     2161 tggggttgag atcgacccct atcgcgcgcc gccccgtcgc gagtgcctcg accagggtgg
     2221 tgccgctgcc gcaaaacgag tcgagaaccg tctctcccgg ggccgtgtgt gcctcaatc
//
LOCUS       DAOSCV010000762         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31070_length_2277_cov_2.849685, whole genome shotgun sequence.
ACCESSION   DAOSCV010000762 DAOSCV010000000
VERSION     DAOSCV010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31070_length_2277_cov_2.849685"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PLY56_15920"
     CDS             complement(<1..975)
                     /locus_tag="PLY56_15920"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ23015.1"
                     /translation="MRLGRVRGITIDIHMSWIVIFALVTYSLGAGYFRVTFPEWGGGV
                     LWSVSLLSAVLFFACVVLHELGHSLTAQKHGISVHGITLFLFGGVARMDREPETPQVE
                     MRVALAGPLVSVLLALGFGALGWAFGGLGGKHPVAMVSLYLALVNGMVVIFNMVPGFP
                     LDGGRALRAALWWYTGSVARATRLAAASGKGFGAFLIASGILLAFAGNLFNGTWFVLV
                     GWFLADAAGTAYAQTLSQHALSGTPLSEVVPWPDEGVTGETSIQTLVDTRLGYNSPDP
                     VPVVENGRVQGLVGIPEVKRIAQTEWTSTTAREAMVPVAALGTVSSDRD"
     gene            complement(1030..1239)
                     /locus_tag="PLY56_15925"
     CDS             complement(1030..1239)
                     /locus_tag="PLY56_15925"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HOJ23016.1"
                     /translation="MSITRVLAFTLLLLNVLALYDILRRPVDLGSKLMWIALVWLFPF
                     VGLLLYLLFGRPNLIRAERTGQSQF"
     gene            1477..2112
                     /locus_tag="PLY56_15930"
     CDS             1477..2112
                     /locus_tag="PLY56_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23017.1"
                     /translation="MGSLLLLLSITALVVMEPLGVPRQVVVRQRHDAWVLAYPLGKVE
                     TLLQVVSPIDEDTEAHYYSLIAHQGQRVHVDLHAWAEPGRPPPRLRLEMVGPGLSSQR
                     ASEPSRMEEGDMRRAGPARSSLALQVPVRGQYILVVRAEERRAGAYRIRFRGRGSWRL
                     SALLRYPLQQIDAASLLWPERARAWTWGTAGAGSLFAGLGYLWRRRRAVVR"
BASE COUNT          423 a          800 c          717 g          337 t
ORIGIN      
        1 atcgcggtcg gagctcacgg tgcccagcgc ggccaccggc accatcgcct cgcgcgccgt
       61 tgtgctcgtc cactccgtct gggcgatgcg cttcacctcg ggaatcccca ccaggccctg
      121 cacccgccca ttctccacga cgggcactgg atcgggagag ttgtagccca gccgcgtatc
      181 gaccagggtc tggatcgagg tttccccggt gacgccctca tccggccagg gaaccacctc
      241 ggagaggggc gtgccggaaa gcgcgtgttg cgagagcgtc tgcgcgtagg ccgttccggc
      301 ggcatctgcc agaaaccagc cgactagcac gaaccacgtg ccgttgaaga gattcccggc
      361 gaaggcaagg agaataccgc tggcgatgag gaatgccccg aaccccttgc cagacgcggc
      421 ggccagacgc gtggcccgcg cgacgctgcc cgtataccac cagagcgcgg cgcgcagagc
      481 gcggccacca tccaggggaa accctggaac catgttgaag atgacgacca tgccgttgac
      541 gagcgccagg tagaggctta ccatggcgac cgggtgcttc cccccgagtc ccccaaaggc
      601 ccagccgagg gcaccaaagc ccagggccag caacacgctc accaggggac cggcgagggc
      661 cacacgcatc tccacctggg gtgtctcggg ctctcggtcc atccgggcca cgcctccgaa
      721 gaggaagagg gtgatcccgt gcaccgagat gccgtgcttc tgggccgtga gcgaatgccc
      781 taactcgtga aggacgacgc aggcaaagaa aagtaccgcg gagaggagcg agacgctcca
      841 gaggacgccc ccaccccatt ccgggaacgt cacccggaag tagccggcgc ccagcgaata
      901 ggtgaccagc gcgaagatca caatccacga catgtgaatg tcgatagtga tcccgcgcac
      961 ccttcccaaa cgcatgccat cccctccccc ggacgctcca cagcgcgcca gctgcccccc
     1021 gggcggtcac tagaactgtg attgccccgt tcgctcggcc cggatgagat tggggcgccc
     1081 aaaaagcaga tagagcagca ggccgacgaa cggaaagagc cacaccagcg cgatccacat
     1141 cagcttgctg cccagatcca ccgggcggcg caggatgtcg tacagggcta atacgttcag
     1201 gagcaacaag gtgaacgcca gcacccgcgt gatcgacata gcgccctctc tcctgccgcc
     1261 aagacggcaa aaacctcgcc aaaccgccgc cggaccgcgc cggtcgcaac acactcccga
     1321 gggccaccgg atccgtagcc cccgctcatc tctgtcggtc atacccggca agcgggcgtg
     1381 cgaaacggca cgcccgcgcc cgaccgctgt ttccctgccc tggctcgcgg gaaagaatcg
     1441 gatatgcacc cccatgggtt catccggaag gcaggtgtgg gatcacttct cctgctgctt
     1501 tccatcaccg ccctcgtggt gatggagcca ctcggggtgc cgcgacaggt cgtggtccgg
     1561 cagcggcatg acgcgtgggt gctggcctac cccttgggaa aggtagaaac gctgctacag
     1621 gtcgtttcgc ctatcgatga ggacactgaa gcgcattact attcgctgat cgcgcaccag
     1681 gggcaacggg tgcatgtcga tctgcacgcg tgggccgagc cgggccgacc accccctcgc
     1741 ctccgtctgg aaatggtcgg gcccggcctc tcttctcagc gcgcctctga gccgagccgg
     1801 atggaagagg gtgacatgcg ccgggccgga cccgcgcgca gctcgcttgc cctccaggtg
     1861 ccggttcgag ggcagtacat cctcgtggtc cgggcagagg agcgccgcgc cggcgcttac
     1921 cggatccgct ttcgtggaag agggtcatgg cgcctgtcgg cgctgctccg gtaccctctc
     1981 cagcagatcg atgcggcatc gcttctctgg ccggagcgcg cccgcgcatg gacgtggggc
     2041 acggccggag cgggcagcct ctttgccggg ctcggatacc tctggcggcg ccggcgcgcc
     2101 gtcgtccgat gattcacttc tcagccggcg taaggagctc cagcaggagg cgagcctcgc
     2161 ccggtagcaa ctcgcccgtg cccttcaacc ccggaatgat cccgcagagg atgacacggc
     2221 ctttctcgaa agccccgctc accacgacgg gagcgccctt cgcgtccttc tcgcgcg
//
LOCUS       DAOSCV010000763         2276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31083_length_2276_cov_3.849167, whole genome shotgun sequence.
ACCESSION   DAOSCV010000763 DAOSCV010000000
VERSION     DAOSCV010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31083_length_2276_cov_3.849167"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..926
                     /gene="hisH"
                     /locus_tag="PLY56_15935"
     CDS             303..926
                     /gene="hisH"
                     /locus_tag="PLY56_15935"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOJ23018.1"
                     /translation="MIAIVDYRAGNLTSVLLALEHLGVPSEITSDPAKILAAERVVFP
                     GDGAAGESMRNLRELGLIETLRAVVERGTPFFGICIGMQILFDFSEENDGVACLGFVP
                     GRVTRFQPAERAVKIPQMGWNAVRFRQSHPLFAGIEDESEFYFIHSYYPVPADPAHCY
                     GETEYAGVCFASAVGKGNLFATQFHPERSGRIGLRMLRNFTEWDGRC"
     gene            920..1699
                     /gene="hisF"
                     /locus_tag="PLY56_15940"
     CDS             920..1699
                     /gene="hisF"
                     /locus_tag="PLY56_15940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ23019.1"
                     /translation="MLTKRIIPCLDVRNRKVTKGVRFQGNVDLGDPIEMAVAYSEGGC
                     DELVFYDITASAERRPIDIGMVREVAKVVHIPFAVGGGIETLDDMYRVLLAGAEKVSV
                     NSLAVKNPGIIAEGARAFGSQCIVLGMDPVSVADRSRFPSGYEVTIRGFRERTGMDAL
                     EWAKRAEDLGVGEIVVNSVDADGTRAGFELTLTSLISTNVHIPVVASGGAGVPQHLVD
                     VFTKGHADAAIVASMTHTGEYTIAAIKAELDAAGIPIRKKW"
BASE COUNT          382 a          720 c          808 g          366 t
ORIGIN      
        1 cggtgattta tcgccgcgcg ccgggttgcg cgctgcctcc gaggcttcgg attcccttat
       61 gtcgggatct cagccacagg gccggcaggg gcggagcccc ccggaggcga atactcactc
      121 tgccggcgtg gccgaaccgc aatgggaccg gacggacggt tccctgccag cgcgaaccca
      181 aacccgtagc aaacccctgt gttcgccctg gcgcgtgcgt ttgcccgggg acggctggca
      241 ggcgaggcgc tggtaccgca tcgcccccat caggcggggc cttggccagg catggagtag
      301 cgatgattgc gattgtggac taccgagccg ggaacctgac gagtgttctc ctggcgttgg
      361 agcatctggg ggtgccgagc gagatcacca gcgacccggc gaagattctc gccgcggagc
      421 gcgtggtctt ccccggtgac ggcgcggccg gggagtcgat gcggaacctg cgcgagctgg
      481 ggctgatcga gacgctgcgc gccgtggtgg agcgcggcac gcccttcttc ggcatttgca
      541 tagggatgca gatcctcttc gacttctccg aggagaacga cggcgtggcg tgcctcgggt
      601 tcgtgcccgg gcgggtgacg cgcttccagc ctgcggagcg ggcggtgaag atcccccaga
      661 tgggctggaa cgcggtgcgc ttccggcaga gccacccgct cttcgcgggc attgaggatg
      721 agagcgagtt ctacttcatc cacagctact accccgttcc ggcggatccg gcgcactgct
      781 acggcgagac agagtacgcg ggcgtgtgct ttgcctctgc cgtgggcaag ggcaacctct
      841 tcgccactca gttccacccc gagcgctcgg gacgcatcgg gctgagaatg ctgcgcaact
      901 tcacggagtg ggacggacga tgctgaccaa gagaatcatc ccttgcctgg acgtgcgcaa
      961 ccgcaaggtg accaagggcg tgaggttcca ggggaacgtg gacctgggcg accccattga
     1021 gatggccgtg gcctacagcg agggcggctg cgacgagctg gtcttctatg acatcactgc
     1081 gtcggcggag cgccgcccca ttgacatcgg catggtgcgt gaggtggcga aggtggtgca
     1141 catccccttc gctgttggcg gcggcatcga gacgctcgac gacatgtacc gcgtgctcct
     1201 ggccggcgcc gagaaggtct ccgtgaactc gctggcggtg aagaaccccg ggatcatcgc
     1261 cgagggcgcc agggcgtttg gctcgcagtg catcgtcctt ggcatggacc ccgtgagcgt
     1321 cgccgatcgc tcccgcttcc ccagcggcta cgaggtcacc atccgcggct tccgcgagcg
     1381 gacgggaatg gacgccctgg agtgggccaa gcgcgccgag gacctgggcg tgggcgagat
     1441 cgtcgtgaac tccgtggatg ccgacggcac gcgtgctggc ttcgagctga cgctcacgtc
     1501 gctcatctcg acgaacgtgc acattcccgt cgtcgcctct gggggcgcgg gcgtgccaca
     1561 gcacctggtg gacgtcttca ccaaggggca tgcggacgcc gccatcgtcg ccagtatgac
     1621 gcacaccggc gagtacacca tcgccgccat caaggcggag ctggacgcgg cggggatccc
     1681 tatccggaag aagtggtaac tgagcgcctc gggagcgcgg gcatgagcca tcggcatgtt
     1741 ggccgggtgg cgcacgcgcc cgccctgcgc ggggacgtct ggcccgaagt attctggtga
     1801 atacttcgag ctagggccgt gtgccgtaga tcacatcccg tcatggtcag gtgccgggcg
     1861 ggcatgcttg cgccgggagc acgaggcaca ggtgcgaaag tggcctgcaa gagccggcgc
     1921 agatacactg ccaaaaccac aaccgacacc gatgccggca agggcagccc tccaaggcaa
     1981 tccaccgatg cagggctgct gggggggggt ggatagggag cgttcccctc ccccggcggc
     2041 gaccgcgcga gcgccacaga gagcaggaag gagcggccga gcatcgaaat cgatggaaag
     2101 cgccgcgcac gcagggtggc gcagtgccgg cctgtcgcca cggtgggccg gcccggctct
     2161 catggttcgg ggggactgat ggccgacgac atcaccatcc actctcttgc tgagaccgag
     2221 aggacgcccg cagagcttgc ccagctgttc aacaccgcct ttggcgagta tgaagg
//
LOCUS       DAOSCV010000764         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31171_length_2272_cov_3.177267, whole genome shotgun sequence.
ACCESSION   DAOSCV010000764 DAOSCV010000000
VERSION     DAOSCV010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31171_length_2272_cov_3.177267"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2272
                     /locus_tag="PLY56_15945"
     CDS             <1..>2272
                     /locus_tag="PLY56_15945"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOJ23020.1"
                     /translation="DARADALLTHKFPIHSYKEPTFDLGPDFNWHVHPIIDVEWPTKI
                     HRCFHWSTLAAAYRQTGNEKYAEEIVQQLLDFGKDNPMEPWSPERRRFAWSTLNATVR
                     IYSSWIDSWLQIRESPHWTADAQFVFLTLLREHGRFLMEKQAKHGNWVVAEAQGLVEL
                     GIMFPEFKEAKTWRDEGYRRLQRELEIQVLSDGVHVERTPGYHLMTLNCFMRPVQLGL
                     LNKVDVPGRDRFVQKLEQMHEFYLYGSKPNRRMEQIGDSGMMAVDSPLRRGWQMFRRE
                     DMLWVLTEGREGKPPVHRSYAFSGGGFYVSRSAWNDPNALWSIVDWGGFLGHCHEDMG
                     HLSVYAYGTELLIDTGIYAYAWPIRAPFYQTSGHNTVMVDEKTQKRRDPLSAKWVSTD
                     QFDLFYGTTDNSEPLIHERTVAFRQPNEAGPGYWLVVDRLTGEGKHRLDQRWHATERL
                     TAKAEGATVTLTGKPESEAQPSLVIAGLPQPGMQTAIVDGAVSYSWYKKTPVEVTQFT
                     LDQEMPAGFVTLLYPTPPGVPPAKVTLEPVTATRDGGQGAVTAVVATIVDRDRRFRDL
                     WLVNHAGEGVIRVGDLETDARVLFFREEGAKRSWFLAEGSFLRKAGETLFSARVKVDG
                     AGAAPRGNATELACTGGEQLLFAATGPATLNGAPFTGERREKAVYVPALEPRAVPEPP
                     RAPGGPRFEIEPPPPPVQASMTLQVLPPDAALPAGSVRVEAEEFSAQGGGGVEVTDRK
                     VGASGRSFFHWDHAGH"
BASE COUNT          384 a          756 c          771 g          361 t
ORIGIN      
        1 cggatgcgcg cgccgatgcg ctcctgaccc acaagtttcc aatccactcc tacaaggagc
       61 ccacgttcga tctggggccc gatttcaact ggcacgtcca cccgattatc gatgtcgagt
      121 ggcccaccaa gatccatcgg tgcttccact ggtccaccct cgccgccgcc taccggcaga
      181 ctggaaacga gaagtatgcc gaggagatcg tgcagcagct gctcgacttc ggcaaggaca
      241 acccgatgga gccctggagc cccgagcgcc ggcgcttcgc ctggagcact ctgaacgcta
      301 ccgttcgcat ctattcgagc tggatcgata gctggctgca gatccgggaa tcgccccact
      361 ggaccgccga tgcccagttc gtcttcctca ccctcctgcg agagcacggg cggttcctga
      421 tggagaagca ggcgaagcac ggcaactggg tcgtcgcgga ggctcagggc ctggtcgagc
      481 tcgggatcat gttccccgag ttcaaagagg cgaagacgtg gcgcgacgag ggctaccgcc
      541 ggctgcagcg cgagctcgag atacaggttc tctcggatgg cgtgcacgtg gagcgcacgc
      601 ctggctacca cctcatgacg ctcaactgct tcatgcggcc ggtgcagctc gggcttctca
      661 acaaggtgga tgtgcctggt cgcgaccgct tcgtccagaa gctggagcag atgcacgagt
      721 tctacctcta tggcagcaag ccgaaccggc gcatggagca gattggtgat agcggcatga
      781 tggccgtgga tagccccctc cggcgcgggt ggcagatgtt ccggcgcgag gatatgctct
      841 gggtgctcac cgagggccgg gagggcaagc cgccggtgca tcgctcctac gctttcagcg
      901 gcggcggatt ctacgtgagc cgcagcgcct ggaacgaccc gaacgccctg tggagcattg
      961 tcgactgggg cggcttcctc ggccactgcc acgaggatat gggacacctc agcgtctacg
     1021 cctatggtac cgagctcctc atcgataccg gcatctatgc ctacgcctgg ccgattcgcg
     1081 cgcccttcta ccagacctcg gggcacaaca ccgtgatggt cgatgagaag acgcaaaagc
     1141 ggcgcgatcc gctctccgcg aaatgggtga gcaccgacca gttcgacctc ttctacggga
     1201 ccacggacaa ctcggagccg ctcatccacg agcgcacggt ggccttccgc cagcccaacg
     1261 aggccggccc cggctactgg ctggtggtgg atcgcctcac cggggagggg aagcaccggc
     1321 tggaccagcg ctggcacgcc accgagcgcc tcaccgccaa ggcggagggc gcgacggtga
     1381 ccctgacggg caagccagag agcgaggcac agccctcgct ggtgatcgcc gggctccctc
     1441 agccagggat gcagaccgcc atcgtcgacg gcgcggtgag ctactcgtgg tacaagaaga
     1501 ctcccgtgga ggtgactcag ttcaccctgg accaggagat gcccgcgggc ttcgtgacgc
     1561 tcctctatcc cacgccgccg ggcgtgcccc ccgcgaaggt gactctggag ccggtcacgg
     1621 ccacccgaga cggcggccag ggcgcggtga ccgcggtggt ggccacgatc gttgaccgcg
     1681 accgccgctt tcgcgacctg tggctcgtga atcatgccgg tgagggcgtg atccgcgtgg
     1741 gtgacctgga gacggatgcc cgcgtcctct tcttccgcga agagggagcg aagcggagct
     1801 ggttcctggc cgaagggagc ttcctgcgca aagcgggaga gacgctcttt agcgcccgtg
     1861 tgaaggtgga tggcgcgggc gccgcgccgc gcgggaacgc gacggagctc gcctgcaccg
     1921 gcggcgagca gctcctcttc gcggccaccg gcccggccac tttgaacggc gctcccttca
     1981 cgggcgagcg tcgggagaag gcggtctacg tgccggcgct ggagccacgc gcggttccgg
     2041 agccaccccg cgcgcccggc ggaccccggt tcgagatcga gccgcccccg cctccggtgc
     2101 aggcctcgat gacgctgcag gtgctcccgc ctgatgccgc gctcccggcg ggaagcgtcc
     2161 gcgttgaggc cgaggagttc tcggcccagg gtggcggggg cgtggaagtg acggatcgca
     2221 aggtgggcgc gagtgggcgc tccttcttcc actgggatca tgccgggcac tg
//
LOCUS       DAOSCV010000765         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31173_length_2272_cov_2.923320, whole genome shotgun sequence.
ACCESSION   DAOSCV010000765 DAOSCV010000000
VERSION     DAOSCV010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31173_length_2272_cov_2.923320"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLY56_15950"
     CDS             <1..968
                     /locus_tag="PLY56_15950"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ23021.1"
                     /translation="NTVRAALDSLARDGFVRHEGRRGTVVNSAFGGGADATFLLVSPA
                     VEEHSPSLLTPEAMALIGNTLCACSGSNIQFHFQAMPPEGARELVRIVHGARLAGVLL
                     VECSEPAVLEALHDEGIPHVVINQEQDMPGPATRVDFWQIGRHAAEFLLDLGHRRLGV
                     LSGPSDRHLYGRMVAGFRDRLAEAGGSLRPEHVAHVKSCSEAARSAALQLLSRPDRPT
                     AVFCTRDVRAYGAYLAARDLGLRVPEDLSLVGYDDITWPGEGRQFLTTFPEPARELGT
                     SAIHMLISWIRTGIVPEDVIICPELVIRRSAAPCSEEGDADDGNR"
     gene            955..>2272
                     /locus_tag="PLY56_15955"
     CDS             955..>2272
                     /locus_tag="PLY56_15955"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ23022.1"
                     /translation="MATGESEVLALFGGPKAVQSNPGDMFDWPIVTKEDEEAILDVLH
                     RRAMSGSDITQLFEKEFAEWQGTQYALGFSSGTAALQAAMYACGVGVGDEIICPSLTY
                     WASCLQAYSLGGTVVFAEVDPQTLCIDPADIEHRITDRTKAIVAVHYLGYPADMDPIL
                     EIARKHGVKVIEDVSHAQGGLYKGRKVGTLGDVAAMSLMSGKSLAIGEAGILATNDLE
                     IYERALSLGHYERFGSNIQTEYLKPHVGLPLGGYKYRMHQMSSAMGRVQLRHYDERMT
                     EIQKAMNYFWDCLEGVPGVKAHRPPKGSGSTMGGWYAAHGLYCPEELGGLSVTRFCQA
                     VSAEGCGISPGANKPLHSHALFNETDVYGHGKPTRIANSNRDLRQPVGSLPVTEQIPS
                     RCYSIPWFKHYRPQIIEEYALAFRKVAENYERLLEDDPGNPDSLGGW"
BASE COUNT          401 a          702 c          757 g          412 t
ORIGIN      
        1 gaaacaccgt gcgcgcggcg ttggactctc tggcacgaga tggcttcgtc cggcacgagg
       61 gacggcgagg gacggtggtg aacagcgcct ttggcggtgg agcagacgcc acgttcttgt
      121 tggtatcgcc ggcggtggaa gaacactccc cctccctgct gacccccgag gcgatggcgc
      181 tcatcggcaa cacgctctgt gcctgcagcg gctccaacat tcagttccat ttccaggcga
      241 tgccgccgga gggcgcgcgc gagttggtgc gcatcgtcca cggtgcccgg ttggccggtg
      301 tgctcctggt cgagtgttca gagccggcgg ttctggaagc attgcacgat gagggcatcc
      361 cgcacgttgt catcaaccag gagcaggata tgcctgggcc ggcgacgcgc gtcgatttct
      421 ggcagatcgg tcggcatgcc gccgaattcc tgctcgatct ggggcaccgg cggttggggg
      481 tgctctcggg accaagtgac cgtcacctct atgggcgcat ggtggccggc ttccgagacc
      541 ggctggctga agcgggaggc agcctccgcc cggagcacgt ggcccacgtg aaatcatgca
      601 gtgaggcggc acgaagcgcc gcgcttcaac tcctctcgcg ccctgaccgg cccacggcgg
      661 tcttctgcac tcgcgatgtg cgcgcttatg gcgcctacct ggccgcacgg gatcttggcc
      721 tgcgagtgcc cgaggatctc tcgctcgtcg gctacgacga tatcacgtgg cccggcgagg
      781 gccggcagtt cctcacgacg ttcccagagc cggcgcgtga actgggcacg tccgctatcc
      841 acatgctgat ctcctggatt cgcaccggga tcgtcccgga agacgtgatc atctgcccgg
      901 agctggtgat ccgacgaagc gctgccccat gcagcgagga aggtgatgca gacgatggca
      961 accggtgaaa gtgaagtgct cgcgctgttt ggcgggccga aggccgtaca gtccaatccc
     1021 ggcgatatgt tcgattggcc gatcgtcacg aaggaggacg aagaggccat tctggatgtt
     1081 ctgcaccgga gggcgatgtc gggaagcgac atcacccagc tcttcgagaa ggagttcgcg
     1141 gagtggcagg ggacgcagta tgcccttggc tttagctccg gcactgcggc gctgcaggcg
     1201 gcgatgtacg cctgtggcgt gggcgtcggc gacgagatca tctgccccag cctcacctac
     1261 tgggcgtcct gtctccaggc ctactccctg ggcggcaccg tcgtctttgc cgaggtcgat
     1321 ccgcagactc tctgcatcga cccagccgat atcgagcatc gcatcaccga tcgcacgaag
     1381 gcgattgtgg cggttcacta cctcggctat ccggcggata tggatccgat cctggagatc
     1441 gcgcgcaagc acggcgtgaa ggtgatcgaa gacgtctctc atgcccaggg cgggctctac
     1501 aaggggcgca aggtaggcac cctcggtgac gtggcggcca tgtcgctgat gagcggcaag
     1561 tcgctcgcca ttggcgaagc cgggattctg gccaccaacg acctggagat ctacgagcgg
     1621 gcgctctcgc tcgggcacta cgagcgcttt ggctccaaca ttcagacgga gtatctgaag
     1681 ccgcacgttg gcctcccgct gggcggctac aagtatcgga tgcatcagat gagctcggcg
     1741 atggggcgcg ttcagctccg gcattatgac gagcgcatga ctgagatcca gaaggcgatg
     1801 aactacttct gggattgcct ggagggcgtg cccggtgtca aggcgcaccg gcccccaaag
     1861 gggagtggca gcacgatggg aggctggtat gccgcgcacg gcctctattg ccccgaggag
     1921 ctgggcgggc tctccgtgac gcgcttctgc caggcagtga gcgccgaagg ctgtggcatc
     1981 agccctggtg ccaacaagcc gctgcattcg catgccctct tcaatgagac ggatgtttac
     2041 ggacacggca agcccacgcg cattgccaac tccaaccgcg atctgcgcca gccagtcgga
     2101 agcctcccgg tgaccgagca gataccctcg cgctgctact cgatcccgtg gttcaagcac
     2161 tatcgccctc agatcatcga ggagtacgcg cttgctttcc ggaaggtcgc ggaaaactac
     2221 gagcggctgc tggaagatga tccagggaac cccgatagcc tgggaggctg gc
//
LOCUS       DAOSCV010000766         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31247_length_2268_cov_2.875734, whole genome shotgun sequence.
ACCESSION   DAOSCV010000766 DAOSCV010000000
VERSION     DAOSCV010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31247_length_2268_cov_2.875734"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="PLY56_15960"
     CDS             <1..1439
                     /locus_tag="PLY56_15960"
                     /inference="COORDINATES: protein motif:HMM:NF025700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HOJ23023.1"
                     /translation="PVAASRNQTLVYGGTPASQEIEALAATALRALPDSRVLVVVPVE
                     APAYALPPAYAAAFFRAPVIPADSRGIPAPVRAALEGRRGLRIFLGGPDGLIPESVAA
                     GLRRYGTVQRVASGDIYRHALLWARGRWDDFGWGINERFQRDGYPCFALANPETPDFA
                     AAGLPLAYRGNNGPLLYTPRDRLDGLTEQYLWRLSPDYFVTPSEGPFINLRILGGPDA
                     VSYSAQARADLASEVRAYRNQGAGMSGLALLGWAWFFIGLAGSIWALFAMPARLPETG
                     FYPRLSWPLAILVLSPVGIIAFLLCYQGRPVSYDEGKPVYVRPLWARTLSATTMSLGA
                     GLALMIAIFYLMQLNGLPLFTTFAGTRLFWLGSPLAAWMWALMVIPAFFLSTLLFTGP
                     MMAEMAGTRYRGGVRLAAPVVALSLVTASLGMWTLVWYWMNFHPLLAEEDRWLWITPL
                     WWGGTLGYLTALIPNYLMERAGWMGGGS"
     gene            1441..>2268
                     /locus_tag="PLY56_15965"
     CDS             1441..>2268
                     /locus_tag="PLY56_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23024.1"
                     /translation="MVRPSLTLLAVAVLLALGLAWITLTWGGSGAPLTAAPNQNAELM
                     ELTGTTARLSGQTHLRTAVAYAQAVYAANQDPDRPGAVLLVRDDDPAAAIAATRLQQV
                     PVNAPMLFVTDGGTRVPDATMDELLRLNPKGVMVDNNVQVYLAGKIPESVQQQVEKAG
                     FRVRRLFAENAVALAEELDEFLAVLKSNHSEEILIAATQAIDYAFAGATWNAHHGSAL
                     AFVTPDGVPLGVLDAWLWTRDRETFGEDKRHWPIEAKESMRWLEGFARCAELAETLPD
                     "
BASE COUNT          323 a          779 c          746 g          420 t
ORIGIN      
        1 cgccggtggc cgcatcgcgc aaccaaacgc tcgtctatgg cggcacgcca gcgtctcagg
       61 agattgaggc gttggcagcc accgccctgc gtgccctgcc ggatagtcgc gtgctggtcg
      121 tggttccggt cgaggcgcct gcatacgcgc tcccgccggc ttatgcggcc gccttcttcc
      181 gcgcgccggt catccccgcc gattcccggg gcatccctgc cccggtgcgc gccgcactgg
      241 agggccgccg gggcctgcgc atcttcctcg gcgggccgga tggcctgatt cccgagagcg
      301 tcgcggccgg gctccgccgt tacggcacgg tgcagcgcgt agcgagtggc gacatctatc
      361 ggcacgcgct cctctgggca cggggccggt gggatgattt cgggtggggc atcaacgagc
      421 gcttccagcg cgatggctat ccctgcttcg ctctggccaa cccagagacg cccgatttcg
      481 ccgccgcagg actgccactc gcctatcgcg gaaataacgg cccgctgctc tacacgccac
      541 gggatcgtct ggatgggctc accgagcagt acctgtggcg gctctcgccc gattactttg
      601 tcaccccctc ggagggtccc tttatcaacc tgcgcatcct cggggggccc gacgccgtga
      661 gctattcggc acaggcgcgc gccgatctgg ccagtgaagt gcgcgcctac cgaaaccagg
      721 gagccggaat gagcggcctg gcgctgctcg gctgggcctg gttcttcatt gggctggccg
      781 gctccatctg ggcgctcttt gccatgcccg cgcgtctgcc ggagacgggc ttctatcccc
      841 gcctctcctg gcccctggcg atcctcgtgc tgagcccggt gggcatcatc gctttccttc
      901 tgtgctatca gggccgtccc gtcagctacg acgagggaaa gccggtctac gtgcgccccc
      961 tctgggctcg taccctctcg gcgaccacca tgagcctggg cgccggcctg gcgctcatga
     1021 tcgcgatctt ctacctcatg cagctgaatg ggctcccact tttcacgacg ttcgccggca
     1081 cgcgcctctt ctggctagga tcgcctctgg cggcatggat gtgggcgctg atggttatcc
     1141 cggcgttctt cctctccact ctcctcttca ccgggccgat gatggccgag atggcgggca
     1201 cccgctaccg gggcggggtg cgccttgccg cgccggttgt cgcgctctct ttggtcacgg
     1261 cgtcgttggg aatgtggaca ctcgtctggt actggatgaa cttccatccc ttgctggcgg
     1321 aggaggatcg atggctctgg atcacgcctc tttggtgggg cggaacgctc gggtacctca
     1381 cggcgctgat cccgaactac ctgatggaac gcgccgggtg gatgggcgga gggtcctagg
     1441 atggtacgcc cgtcgctgac actcctggca gtcgccgttc tcctcgcgct tggcctggcc
     1501 tggatcacgc tgacctgggg gggctccggg gcgcccctga cagccgcccc caaccagaac
     1561 gcggagctga tggagctgac cggcaccacg gcccgacttt ctggacagac ccacctgcgc
     1621 acggcggttg cctatgccca ggcggtctac gccgcgaatc aggacccgga tcggcctggg
     1681 gcggtgctcc tcgtgcgcga tgatgaccct gcagccgcca tcgccgccac gcgcctgcaa
     1741 caggtgccgg tgaacgcccc catgctcttt gtcacggatg gaggcacccg ggtgccggat
     1801 gccacgatgg atgagctgct gcgcctcaac cccaaggggg tgatggtgga caacaacgtt
     1861 caggtctatc ttgccgggaa gattcccgag agcgtgcagc agcaagtgga gaaagccggt
     1921 ttccgcgtgc gccgcctctt cgcggagaat gccgtggccc tggccgagga gctggatgaa
     1981 ttcctggccg tgctcaaatc gaaccactcc gaggagatcc tcatcgccgc tacccaggca
     2041 atcgattatg ccttcgccgg ggcaacctgg aacgcacacc acggcagcgc gcttgccttc
     2101 gtcaccccgg atggcgtgcc gctgggggtg ctggacgcct ggctgtggac ccgcgaccgg
     2161 gaaaccttcg gcgaagacaa gcggcactgg ccgatcgagg ccaaggaaag catgcgctgg
     2221 ctggaaggct ttgcgcgctg cgccgaactg gcggagaccc tgcccgac
//
LOCUS       DAOSCV010000767         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31326_length_2263_cov_3.669384, whole genome shotgun sequence.
ACCESSION   DAOSCV010000767 DAOSCV010000000
VERSION     DAOSCV010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31326_length_2263_cov_3.669384"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..549
                     /locus_tag="PLY56_15970"
     CDS             268..549
                     /locus_tag="PLY56_15970"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ23025.1"
                     /translation="MIVGIARIELEIPGSNSLKDRRQVVKSLVGHLQSKFHISVAEVD
                     DPQVWRRANIGVALVGTDTAFVNRVLDKVVDAVRGDPRVSIIDYEIEML"
     gene            601..969
                     /gene="rbfA"
                     /locus_tag="PLY56_15975"
     CDS             601..969
                     /gene="rbfA"
                     /locus_tag="PLY56_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ23026.1"
                     /translation="MATTRQRRVAELIKEEVSQILQHEVSDPRIGFATVTDVEVTADL
                     REARVFISVLGDPEQAQESIAALQRAAGYIRRLLGRRVDLRVIPELTFALDRSLERGS
                     RVLQLLHELEQEQSGKAKAE"
     gene            966..1952
                     /locus_tag="PLY56_15980"
     CDS             966..1952
                     /locus_tag="PLY56_15980"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ23027.1"
                     /translation="MIGDPLEMAAEALHSAAKVLLACHVNPDGDTMGSALALGLALHS
                     AGKEVTLVCADEVPLPYRFMPGSDSFLRAVPEEAFDVAVALDCDGERRLGSLAPAVRR
                     APCVIEIDHHTGTDRFGDIVAVDSTAAATAVLVYELIRLLAQPITADIATNLFVGIMT
                     DTGSFRFANTRARSLRIAAELVDCGADPYQIADHVYETRSRGATLLLGQALNSLRLYQ
                     EGQIATARLTAEDFESAGASDADTEGIVNHVRAIAGVQIALLLREINGKVRVSLRSRE
                     PVDVAEIAREFGGGGHRVAAGCTIPLPIAQAEERLLDAARRGLQRAHSSPRP"
     gene            1960..>2263
                     /locus_tag="PLY56_15985"
     CDS             1960..>2263
                     /locus_tag="PLY56_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007077490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ23028.1"
                     /translation="MDGILILHKPVGPTSHDMVSRVRRLLHTRRVGHAGTLDPPAEGV
                     LVLFIGRATRLIEYTADADKEYVAECCFGAATDTLDASGTVTATADASGLTREAVEE"
BASE COUNT          379 a          739 c          759 g          386 t
ORIGIN      
        1 ggcaaggaga ccatcttcac cgggaagctc tcttcgctgc gccatgtcaa ggatgacgtg
       61 cgcgagatgg cggccggctt cgagtgcggt atcatgctgg atggtttctc cgacttccag
      121 gaaggggata tcatacagtc gtacgtggtg gaggaggtcc accgcgcctg agagggcgcc
      181 ggaagaggcg tatcggccga gcgcggcgtc gggcgtgttg ccctgccgcg ctcggcaggc
      241 actcggagcc gtttcgcggg ggagaggatg atcgtcggca tcgcccggat cgagttggaa
      301 atcccgggga gcaactcgct caaggatcgg cgccaggtgg tcaagagctt ggtcggccat
      361 ctgcagagca agttccacat ctcggttgcc gaggtagacg atccgcaggt gtggcggcgc
      421 gccaacatcg gtgtcgcgct tgtcggcacc gataccgcgt tcgtcaaccg ggttctcgac
      481 aaagtggtcg atgccgtgcg gggcgacccg cgcgtgtcga tcatcgacta tgagattgag
      541 atgctgtgag aggttcgtgc ccggccaagc cgggatccga acccgtggta aggggagagc
      601 gtggcaacta cgagacagcg tcgggtagcc gagctgatca aggaggaagt cagccagatc
      661 ctccagcacg aggtgagtga cccgcgcatc ggattcgcca ccgtgacaga tgtcgaggtg
      721 actgcggatc ttcgcgaagc gcgcgtcttc atcagcgttc tgggcgatcc ggagcaggca
      781 caggaaagca tcgccgcgct gcagcgagcg gccggctaca tccggcggct cctggggcgc
      841 cgcgtcgatc tgcgcgtcat tccggaactt accttcgcgc tcgaccgttc tctggaacga
      901 ggctcgcgcg ttctccagct gctgcatgag ctggagcagg agcaatccgg gaaggcaaag
      961 gcggaatgat cggggatccc ctggagatgg ccgcggaggc gttgcactcc gcggcgaagg
     1021 tcctgctggc ctgtcacgtc aacccagatg gcgataccat gggcagcgcg ctggctctgg
     1081 gtctggcgct tcactccgcc ggcaaggagg tgaccctcgt ctgcgcggat gaggtacccc
     1141 tcccctaccg cttcatgcca ggatccgact cttttctccg cgccgttccc gaagaggcgt
     1201 tcgatgtcgc cgtcgcgctc gattgcgatg gcgaacgccg cctgggcagc ctcgcgcccg
     1261 cagtgcgccg cgctccctgc gtgattgaga tcgatcacca caccggcacc gaccgcttcg
     1321 gcgatatcgt ggcggtagat tcgacagcgg ccgccaccgc ggtcctcgtc tatgagctga
     1381 tccgcctcct ggcacagcca atcacggccg acatcgccac caacctcttc gtcgggatca
     1441 tgaccgacac gggctccttc cgcttcgcga acacgcgagc gcgctccttg cgcatcgccg
     1501 ccgagctcgt ggattgcggc gcggatccct accagattgc agaccacgtc tatgaaaccc
     1561 gctcgcgcgg ggccacgttg ctcctgggcc aggcgctcaa ctcgttgaga ctctaccaag
     1621 aggggcagat cgccacggcc cgcctgactg cggaagattt cgagagcgcg ggtgccagcg
     1681 acgccgacac agaggggatc gtcaaccacg tgcgggcgat tgcgggcgtg cagatcgcac
     1741 tcctcctccg cgagatcaac ggcaaggtgc gcgtcagcct gcgctcgcgc gagccggttg
     1801 atgtcgccga gatcgcgcgc gagttcggcg gtggcggcca tcgcgtcgca gccgggtgca
     1861 ctatccccct gcccatcgct caggcggagg agcgcctgct ggatgccgcc cgacgcgggc
     1921 ttcagcgggc acactccagc ccccgcccat aggcttccca tggacggcat cctgatcctc
     1981 cacaagccgg tcggaccgac ctcacacgat atggtctcgc gcgtgcgccg gttgctgcat
     2041 acgcgccgtg ttgggcacgc cggcaccctg gacccgccgg ccgagggcgt tctggtcctc
     2101 tttatcggtc gcgccactcg cctgatcgag tataccgccg acgccgacaa ggagtacgtc
     2161 gccgagtgct gcttcggcgc cgccaccgat accctggatg ccagcggaac ggtcaccgcc
     2221 acggcggatg cctcggggct gacgcgcgag gcagtcgagg agg
//
LOCUS       DAOSCV010000768         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31359_length_2262_cov_2.880834, whole genome shotgun sequence.
ACCESSION   DAOSCV010000768 DAOSCV010000000
VERSION     DAOSCV010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31359_length_2262_cov_2.880834"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLY56_15990"
     CDS             complement(<1..774)
                     /locus_tag="PLY56_15990"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOJ23029.1"
                     /translation="MRAAGVELRQSRVAEEEEPMFQLCLLRSEQIEPLAEGVLQVLAR
                     VGVLCENEEMCRALREWGAEVDFAAQRVRFPRRQVEAFIEQLRREEAGRQPEWGTHFP
                     PPSLPGIGTQVAQITHDFETGEQRNACTRDFITFTQLGAVLHPEKPVGHSLSLTDVPP
                     VMEPLEAGLLLAEWAPIPGLPFIWRADQADYLNEMGQLLGIDHWFTWGATCFAHPLRF
                     DRDTAGKFVRRVREGASAGLTAMPVAGASTPITVEGFIVV"
     gene            complement(896..1378)
                     /locus_tag="PLY56_15995"
     CDS             complement(896..1378)
                     /locus_tag="PLY56_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23030.1"
                     /translation="MIELRAAHPSARTRKGPAAVLCERCGTDNPEGTVVCVQCYYAVG
                     IPYNVGSSKGGKKGSKEPPAWAVSLGLNDIQTRQISTQLGNLAQESVAGFWFASHDDA
                     SETQKALADTIGAEIAAKKVKLATAEVNGRTVLFIAGKVEDPQMLQMVCESFNGQVAF
                     "
     gene            complement(1437..1766)
                     /locus_tag="PLY56_16000"
     CDS             complement(1437..1766)
                     /locus_tag="PLY56_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOJ23031.1"
                     /translation="MQGIVKSFDRNRGFGFITTENGDEIFVHHTDIADSTREYLTPGQ
                     GVRFNVMETERGPRAVKVHITQEVPLEKERRLDWRGRRHGRPRPGKVPKAVLRARGIL
                     PEEEEDE"
     gene            complement(1766..>2262)
                     /locus_tag="PLY56_16005"
     CDS             complement(1766..>2262)
                     /locus_tag="PLY56_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23032.1"
                     /translation="RLEGIFKILPKGAILPGDPFASLGQHFTAEVLGTSRVAGRPTYR
                     LKLTPKGEGPTHPVYAHVDRERWNVLQMTSATPGGDAITVEMTYQRVRGRFWLPSRTV
                     ARFRLPDIDAPSNAPGDDSGFRMAGQRGTVTATFRNYKVNTGLADSLFREDAQARQDR
                     QRRH"
BASE COUNT          364 a          723 c          748 g          427 t
ORIGIN      
        1 gacgacgatg aagccctcga cggtgatcgg cgtcgaggct ccggcgaccg gcatggcggt
       61 cagcccggcg gaggcaccct cgcgcactcg tcggacgaac ttgcccgcgg tatcgcgatc
      121 gaagcgcagg gggtgggcaa agcaggtggc accccaggtg aaccagtggt cgatgccaag
      181 aagctggccc atctcgttca ggtaatcggc ctgatccgcg cgccagataa aggggagacc
      241 ggggatgggt gcccactcgg ccagcagcag tcccgcctcg agcggctcca tcacgggggg
      301 aacatcggtg agcgagaggg agtggccgac gggcttctcg gggtggagga ctgcgcccag
      361 ctgcgtgaag gtgatgaaat cgcgcgtgca ggcgttgcgc tgctcgcctg tctcgaagtc
      421 gtgcgtgatc tgggcgacct gagtgccgat gccggggaga gagggcggcg gaaagtgcgt
      481 gccccactct ggctgccggc cggcctcctc gcggcgcagc tgctcgatga acgcttcgac
      541 ctgcctgcgc gggaagcgca cccgttgggc cgcgaaatcg acttcggcgc cccattcgcg
      601 cagggcgcgg cacatctctt cattctcgca cagaacgccc acccgcgcga gaacctggag
      661 cacgccttcg gcaagtggct cgatctgctc gcttctgagc aagcacagct ggaacattgg
      721 ctcctcctct tccgccacgc ggctttgcct cagttccacg cctgcggccc gcatcctgcc
      781 tcgccgagat caatgacgtg tgggatgatg gtggcatgca ccgacgttcc gaccgattgg
      841 gtcgggcggc atcgatgggg agggtagggt gcagatacgg ccctgtgaat cttgctcaga
      901 aagcgacctg gccattgaac gattcgcaga ccatctggag catctgcgga tcctcaacct
      961 tgccggcgat gaagagcacg gtgcggccat tcacctcggc ggtggcgagc ttgaccttct
     1021 tggccgcaat ctcggcgccg atggtatcgg cgagggcctt ctgcgtctcc gaggcgtcgt
     1081 cgtggctggc gaaccaaaag ccggcgacgg actcctgggc caggttgccg agttgcgtgc
     1141 tgatctggcg ggtctggata tcattcaggc ccaggctgac cgcccaggcg ggcggctcct
     1201 tgctgccctt cttgccccct ttgctgctcc cgacgttgta cgggattcct acggcgtagt
     1261 agcactgaac gcagacaaca gttccttccg gattatcggt tccacaacgc tcgcagagca
     1321 cggcagcggg tcctttcctg gtgcgggccg acggatgggc cgcgcggagc tcgatcaatc
     1381 atggctccta ctcagacgct attccccatt agaggcgaat ttcctgctgg agtcttctac
     1441 tcatcttcct cttcttcggg caggatgccg cgcgcccgga gcactgcctt gggcaccttt
     1501 ccgggccgcg ggcgaccgtg gcgcctcccg cgccagtcca aacggcgctc cttttcgagg
     1561 ggcacttcct gagtaatgtg caccttaacc gcccgtggcc cgcgctccgt ctccatcacg
     1621 ttgaagcgca cgccctggcc aggcgtgaga tactcgcgcg tcgagtcggc gatatcggtg
     1681 tgatgcacga agatctcatc gccgttctcc gtggtgatga acccgaagcc acggtttcgg
     1741 tcgaagcttt tcacaatccc ttgcatcagt gcctcctctg gcggtcttgt ctcgcttggg
     1801 cgtcctcgcg gaagagggaa tcggcgaggc cggtgtttac cttatagtta cggaaggtcg
     1861 ccgtcacggt gcctcgctgc ccggccatgc ggaaccccga gtcgtcgcca ggcgcgttgg
     1921 atggcgcgtc gatatcaggc agccggaagc gcgccacggt gcgcgaggga agccagaacc
     1981 gcccgcgcac gcgttggtag gtcatctcca cggtgatggc atcgccccct ggagtcgcgc
     2041 tggtcatctg caggacgttc cagcgctcgc ggtcgacatg ggcgtacacc ggatgcgtgg
     2101 ggccctcccc tttgggggtc agcttcagcc ggtaggtggg acgtcctgcc acgcggctcg
     2161 tgccgagcac ctccgccgtg aagtgttggc ccaggctggc gaagggatcc ccgggcagga
     2221 tggcgccctt aggcaggatc ttgaaaattc cctcgagcct aa
//
LOCUS       DAOSCV010000769         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31370_length_2261_cov_3.862194, whole genome shotgun sequence.
ACCESSION   DAOSCV010000769 DAOSCV010000000
VERSION     DAOSCV010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31370_length_2261_cov_3.862194"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /gene="rfbA"
                     /locus_tag="PLY56_16010"
     CDS             <1..849
                     /gene="rfbA"
                     /locus_tag="PLY56_16010"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOJ23033.1"
                     /translation="GTRLYPLTHVVSKQLMPVYNKPMIYYPLTTLLLAGITDILTITT
                     PQDQPLFEQLLGDGSQWGVRIRYAVQPHPGGIAQAFLIGRDFVGDSPCALILGDNIFF
                     GHGLPELLRAASANEEGATIFAYRVQNPEQYGVVELDQEGRPLSIVEKPARPRSHYAV
                     TGIYFYDHQVVEIASHLKPSARGELEITDLNNLYLERGQLRVQVLGRGFAWLDTGTHE
                     GLLQAANFVATVEERQGLMVACPEEIAYRMGLIGADELRQQAEVLRKNRYGEYLLRLL
                     DEATIR"
     gene            846..1397
                     /gene="rfbC"
                     /locus_tag="PLY56_16015"
     CDS             846..1397
                     /gene="rfbC"
                     /locus_tag="PLY56_16015"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOJ23034.1"
                     /translation="MKVIEGRLPGVLIVEPQVHGDARGFFLETWSRTRYEAAGIRGDF
                     CQDNLSFSRRGTLRGLHYQHPHAQDKLVYVLQGEIFDVAVDIRRGSPTFGQWEGVRLS
                     AENHRQVFVPKGFAHGFCVVSETALVAYKCTDRYHPETEGSILWNDPEIGIDWPLVDV
                     LLSEKDRNAPRLRDVPPERLPTW"
     gene            1420..2172
                     /locus_tag="PLY56_16020"
     CDS             1420..2172
                     /locus_tag="PLY56_16020"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ23035.1"
                     /translation="MLSDHRVPQQNVVQTWDNVARTYDEEEYWRALGSHAILSHLLQH
                     MGDPTGKRIIEVGCGSGFMSLALARRGAQCAVLDISPEALGVARRAFEQAGMPPPECF
                     LTNALDSGIPSHSFDVAWNAGVIEHFSDADKERLVREMIRITKPGGLVIILVPNAWCI
                     PFQLAQACMKWRGRWPYGFEDDMSPRRLRRMCAAMGLRDVETYAFDPVTGIKWMRGGV
                     HIANLLRINTVEHHCRRSLMGQLAILTLRCTS"
BASE COUNT          411 a          728 c          706 g          416 t
ORIGIN      
        1 ggaacccggc tttaccccct cacgcatgtc gtcagcaagc agctcatgcc ggtttacaac
       61 aagccgatga tctactaccc gctgacgaca ctgctgctgg cggggatcac cgatattctc
      121 accatcacca caccgcagga ccagccgctc ttcgagcagc tcctgggcga tggcagccag
      181 tggggggtgc gcatccgcta tgccgtgcag ccgcatccgg gagggatcgc gcaagccttc
      241 ctcatcggcc gcgattttgt gggagattcc ccctgcgcgc tgattctggg cgacaatatc
      301 ttcttcggtc atggcctgcc ggagttgctt cgcgccgctt ccgcgaacga ggagggcgcc
      361 accatcttcg cctaccgggt gcagaacccg gagcagtatg gcgtggtgga actcgatcag
      421 gaaggccggc cgctcagcat cgtggaaaag ccggccagac cacgctcgca ctacgccgtg
      481 accggcatct acttctatga ccaccaggtg gtggagatag cctcacacct caagccctcc
      541 gcgcgcggcg agttggagat cacggatctc aataacctct accttgagcg cggccagctt
      601 cgggtccagg tgctgggccg cggcttcgcc tggctggata ccggcaccca cgagggcctg
      661 ctgcaagccg ccaacttcgt ggccaccgtg gaggagcgtc aggggctcat ggtcgcctgc
      721 cccgaggaga ttgcctaccg catgggcctg attggggcgg acgagctccg ccagcaggcc
      781 gaggtgttgc gcaagaatcg ctatggcgag tatctcttgc gcctgcttga tgaggcaacg
      841 attcgatgaa ggtgattgag ggccggctgc cgggcgtgct gatcgtggaa ccgcaggtcc
      901 acggtgatgc ccgcggcttc ttcctggaga cgtggtcacg cacccgctac gaggccgctg
      961 gaatccgcgg cgatttttgc caggataacc tctccttctc gcgccgcggc acgttgcgtg
     1021 gcctgcatta ccagcacccg cacgcccagg ataagcttgt gtacgtcctg caaggcgaga
     1081 tcttcgatgt ggccgtggat atccgccgcg gctcacccac tttcgggcag tgggagggcg
     1141 tgcgcctctc cgccgaaaac caccgccaag tcttcgtgcc caagggcttt gcccacggct
     1201 tctgcgtggt cagcgagacc gcgctagtgg cctacaagtg cacggatcgc taccacccgg
     1261 agactgaggg cagcatcctc tggaacgacc cggagatcgg catcgactgg cctctggtcg
     1321 acgtgctcct ctccgagaag gaccgtaacg cgccgcgctt gcgcgacgtg ccgccggaac
     1381 gcctgcccac ctggtagacc catcgagggg atgaacccga tgctcagcga tcaccgcgta
     1441 ccccaacaga acgtggtcca gacatgggac aacgtcgcac gcacctacga tgaagaggaa
     1501 tactggaggg cgttggggag tcatgcgatc ttatcgcacc tcctgcagca tatgggcgac
     1561 ccgacaggca agcgtatcat cgaggtgggc tgcggcagcg gcttcatgtc gctcgccctg
     1621 gcaaggcgcg gcgcgcagtg cgccgtgctg gatatctccc cggaagccct gggcgtggcc
     1681 cggcgagcat tcgaacaggc cggcatgccg ccgcccgagt gcttcctgac gaacgccttg
     1741 gactcaggaa tcccctccca ttctttcgat gtggcgtgga acgcgggcgt catcgagcat
     1801 ttctccgacg cggacaagga acgtctagta cgcgagatga tccgcatcac caagcccggc
     1861 ggcttggtga taatccttgt tcccaacgca tggtgcattc cattccagct ggcgcaggcg
     1921 tgcatgaagt ggcgcggcag gtggccctat ggcttcgagg atgacatgag cccccggcgc
     1981 ttgcgccgaa tgtgcgccgc gatgggcttg cgcgacgtcg agacatatgc cttcgacccg
     2041 gtaacgggga taaagtggat gcgcggcgga gtgcatatcg caaacctcct ccgcatcaac
     2101 accgtggagc atcattgccg gcggtccttg atggggcaac tcgcaatcct gactctgcgc
     2161 tgcaccagtt gaaccaccgg ctatgacctt acctgaagta ctcgctccgg ccaagcggct
     2221 tctgcgaacc tcgcgggggg tgcgatgtca gcgtctcgat a
//
LOCUS       DAOSCV010000770         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31463_length_2256_cov_3.422081, whole genome shotgun sequence.
ACCESSION   DAOSCV010000770 DAOSCV010000000
VERSION     DAOSCV010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31463_length_2256_cov_3.422081"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="PLY56_16025"
     CDS             <1..2097
                     /locus_tag="PLY56_16025"
                     /inference="COORDINATES: protein motif:HMM:NF027473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4855 domain-containing protein"
                     /protein_id="HOJ23036.1"
                     /translation="QQPEWSRPKSYEHLSGWLDAIFGERGLLPVLQQAMASAEARLGP
                     PPAPRQVILSIPRPQGTSAARLSLVKWLVDEAIRRWQEASPKNLQLWGMYWYWEDVAG
                     ADRSLARDACAVVHQRGLKMLWIPYYRAAGWDQWREIGFDLAIMQPNYAFLPLHEGRH
                     LPDESRLAETARLAREAGMGVEIETAYALTTSEIDRANLRLYLNHSLEDGYANAVRAH
                     FFSFDQYQRLAVSPLPEARQLYDDLFALARGRLVERSVSLLGNAPVRHIRAGTDVANA
                     ASLIGASGVGMGAGDYIEVTLDSPRILSEMRLCLAAEGDGIGLRSLSIYGRETAEGPW
                     RMLQSLAEAPPTEGSWLIAGWSPQMLSALRVVPDPFPGKRVTITRLAAYPGVSSATSP
                     DNLAFGRSYTVRPAPESHYPDREAELTDGILTRQGFADGHTVGWSGAPRVTVEVALPE
                     ETSVEAVRCHVQGGGYAAVHFPRALSVAISNDGKQWRLLSDSRARIEPAPVDPGVPQE
                     ISLRWLRQEGPPGGLPARWVRLTLLPAGWLMLSEVEVLSGGRNVARGQPYRLRPLPTA
                     KGNYSDDGIKLTDGVAAPLWAGSVGWNGVEGDVEVDLGCLCRVRAIRAYVLGGGTGAV
                     FLPRDIQVSLSTDGRAWTPPVAATLPRPEEKEHTLGVWALAEVNPQKARYVRLHLRPT
                     RGWMMLGEVEVLGTRP"
BASE COUNT          346 a          731 c          776 g          403 t
ORIGIN      
        1 caacagccgg agtggtcccg ccccaagagt tacgagcatc tgagtggttg gctcgatgct
       61 atcttcggcg agaggggcct ccttcccgtc ctgcagcagg cgatggcaag cgccgaagcc
      121 cgccttgggc cgcctccggc gccgcgacag gtgatccttt cgatcccgcg gccccagggt
      181 acctccgcgg cgcgtctctc cttggtgaag tggctcgttg acgaggcgat tcgccgctgg
      241 caggaagcct ctcccaagaa ccttcagctc tgggggatgt actggtactg ggaggatgtg
      301 gccggcgcgg accgctccct ggcgcgggac gcgtgcgcgg tcgtccacca gcgcggcctg
      361 aagatgctct ggattcccta ctatcgcgcc gccggctggg accagtggcg ggagatcggc
      421 ttcgatctgg ccatcatgca gccgaactac gcgtttctgc cccttcacga gggccggcac
      481 ctgcccgatg aaagccgcct cgcggagacc gctcgcctgg cccgtgaggc gggcatgggc
      541 gtggaaatcg agacggccta cgccctgacg acttctgaga tcgaccgtgc caacctgcgc
      601 ctctacctca accactccct ggaggatggt tacgcgaacg ccgtacgcgc gcacttcttc
      661 agcttcgacc agtatcagcg cctggctgtt agtccactcc cggaggcacg ccagctctac
      721 gatgacctct tcgcgcttgc ccgcggtcgc cttgtcgagc gctccgtatc gctgctggga
      781 aatgcaccgg tgcgccacat ccgggcgggg accgatgtag ctaacgcagc ttccttgatc
      841 ggcgcctccg gggtcggcat gggcgcggga gattacatag aggttactct tgattcgccg
      901 cgcatcctat ccgagatgcg cctctgcctg gcggctgagg gagatggcat aggcctgcgc
      961 tccctctcga tttacgggcg ggagacggcc gaaggcccat ggcgcatgct tcagtcgctc
     1021 gctgaggcac cgccaacaga gggaagttgg ctcatcgccg ggtggtcgcc ccagatgctc
     1081 tctgcactgc gcgttgttcc agatcctttc cctgggaagc gcgtcaccat cacccgtctt
     1141 gcggcctatc cgggagtctc cagcgccact tcgccggaca acctcgcatt cggacggagc
     1201 tacaccgtgc ggccggcgcc ggagagccat tatccggatc gcgaggcgga gctgaccgat
     1261 ggcatcctga cccggcaggg gttcgcggac gggcataccg tgggctggag tggcgcgcca
     1321 agggtgacgg tggaggtggc gcttcccgag gagacatcgg tagaggcggt gcgttgccac
     1381 gtgcaggggg gtggatacgc cgccgtccat ttcccgcgag cgctttcagt ggcgatcagc
     1441 aacgatggca agcagtggcg tctccttagc gattccaggg cgcgcatcga gccggcaccc
     1501 gtggatccgg gcgtgccaca ggagatcagc ttgcgatggc tgcgccagga gggtccacca
     1561 ggcggtctcc ctgcccgatg ggtgcggctc actctgctgc cggccggctg gctgatgttg
     1621 agcgaggtcg aggtgctgag tggcgggcgc aacgtagccc ggggccagcc ctaccgactg
     1681 cgtccccttc caaccgccaa ggggaattac tcggatgatg gcatcaagct cacggatggt
     1741 gttgccgctc cgttatgggc cgggtctgtg ggttggaatg gagttgaggg cgatgtggag
     1801 gtggacctcg gctgtctctg ccgggtgcgt gccatccgcg cctacgtgct cggtggcggc
     1861 acgggcgccg tctttctgcc gcgagacatt caagtgagcc tctccacaga cggccgggcc
     1921 tggacgcctc ctgtcgccgc caccctcccc cggccagagg agaaggagca taccctgggc
     1981 gtatgggctc tcgccgaggt gaatccccag aaggcacgct atgtgcgcct ccaccttcgg
     2041 ccgacgcgcg gctggatgat gctcggcgaa gtggaggtcc tgggcacacg gccgtagggc
     2101 atggtgcgcc cgtggcgggc cccccggtgg caaggagaga tcgagaccgc tgtcgtatgc
     2161 tcctcccggt gcgagcttcc cgcgcgctcg tagggacgcc gcgcgccgct atggaaagga
     2221 ggcagccggc agggagtgga gcgggaccaa tgtccc
//
LOCUS       DAOSCV010000771         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31490_length_2255_cov_3.555455, whole genome shotgun sequence.
ACCESSION   DAOSCV010000771 DAOSCV010000000
VERSION     DAOSCV010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31490_length_2255_cov_3.555455"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLY56_16030"
     CDS             <1..222
                     /locus_tag="PLY56_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23037.1"
                     /translation="AFVGGAPSVPVRAGEISVGAWLRSLCARSVYEPFAWNDPMPLAA
                     ALWKRVRPKIPRWARPSQSAPTRGGSAPN"
     gene            237..1037
                     /locus_tag="PLY56_16035"
     CDS             237..1037
                     /locus_tag="PLY56_16035"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ23038.1"
                     /translation="MRWLLLSDLHANLEALEAVLRDAEARWGSLRVACAGDVVGFGPS
                     PNECIALLRERDALCVAGNHDLMVLERIAPRCTPDGLRAVEWTRARLDRSSREWLATL
                     PTAREVSGSFVLCHGALEDPDHYIVDDADAQAQLSRLEACYPGIGLMVCGHTHRAVWA
                     PAGRVRRANAGACFSMAPSVPALVNPGSVGQSRTLERSARYAVLDTAAGTVEYCAVAY
                     DHAACARKIRAAGLETKLYVTLGALLRRRIRRGVGRTAEWLSLRPQSP"
     gene            1246..>2255
                     /locus_tag="PLY56_16040"
     CDS             1246..>2255
                     /locus_tag="PLY56_16040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ23039.1"
                     /translation="MRQEQPGSETADPAGRVEIIEEPRRLEAISTAWDQLAEKDPRAH
                     VFQGCPWLSTWLRHYPRARPFLLVFWRDDEMTAALPLCAYDLGRGWCRMRTLRFIADN
                     NSDYCDALSDPDHPNDLGVLWEHLTRRKDWHLLDLRYLPEGSQVATLSHAAGQRFWSL
                     RQRLDAAPYIDLRTDWRERVPRNHRTEVLRRWRRVQEQGQVTFEIARTPEEADMMLDQ
                     LARMHIARWQGRHETSVFHFADYRRWVHDVCHELLKRDQLYLCRLSLDGRPMSMALAF
                     LQSRRLLRYTSTFDEAYSRFGPVHLLLMAVVEDLRANNIADVHEFGRGGEAYKARWTH
                     QA"
BASE COUNT          363 a          713 c          770 g          409 t
ORIGIN      
        1 gcgttcgtcg gcggtgcgcc atcggtaccc gtgcgcgccg gcgagatcag cgtgggggcc
       61 tggctgcgct cgctctgtgc gcgatccgtt tacgagccgt tcgcctggaa cgatccgatg
      121 ccactggccg cggctctctg gaagcgcgtg cgccccaaga tcccgcgttg ggcgcgacct
      181 tcgcagtctg caccgacgcg aggcggatca gcgccgaact gaagggaccg caacgggtgc
      241 gctggctgct cctctccgat cttcatgcca acctggaggc gctcgaagcc gtcctccgcg
      301 atgccgaggc gcgctggggc tccctccggg ttgcgtgcgc gggcgatgtc gtcggctttg
      361 gaccgagccc caacgagtgc atcgcgctgc tgcgcgaacg tgatgcgctc tgcgtggctg
      421 ggaaccacga cctgatggtc ctggagcgca tcgcaccgcg atgcaccccg gatggcctgc
      481 gcgcagtgga gtggacccgg gcgcgtctcg accggagcag ccgcgaatgg ctcgccacct
      541 tgcccacggc acgcgaggtc tctggatcgt tcgttctgtg ccacggggcg ctggaggatc
      601 cggatcacta catcgtggat gacgcggacg cgcaggccca actgagtcgg cttgaggcgt
      661 gctacccggg gattggtctc atggtgtgcg ggcacacgca ccgtgccgtg tgggcacccg
      721 ccggccgagt gaggcgcgcg aacgcgggcg cctgcttctc gatggctccc tcggtgccgg
      781 cgttggtgaa tccgggaagc gttgggcagt ctcggacgct ggagcgttcc gcccgctatg
      841 ccgtcttgga taccgccgcc ggaaccgtgg agtactgcgc ggtcgcttac gaccacgcgg
      901 cgtgtgcgcg caagattcgc gcggccggcc ttgaaacaaa gctttacgtc acccttggag
      961 ctctcttgag gcgtcggatc cggcgaggcg ttggacgaac cgcggaatgg ctatcgctcc
     1021 ggccgcaatc accatgaaac cccggcgccg cgggctcggc ccggtactca tcttgcagag
     1081 aattgaagag caatccggct ctgccaggcg ccggagtgca taccggaggg gatggaaagg
     1141 gaacgggaac tttgccatcg cacccgttca ggcgcctggc agtggggttg cccacgtatg
     1201 ggcgtcgctt ccagaatacc cggcacgtga agggatactc gattgatgag gcaagagcag
     1261 ccggggagtg agactgctga tcccgccggg cgtgttgaga tcatcgagga gcccaggcgc
     1321 ctcgaggcga tctccacggc ttgggaccag ctggcggaga aggatcctcg ggcccatgtc
     1381 ttccagggtt gcccgtggct ctctacatgg ctgcgccact atccgcgggc acggccattc
     1441 ctcctggtgt tctggcgtga cgacgagatg acggcggcgc taccactctg tgcctacgat
     1501 ctcgggcgcg ggtggtgtcg gatgcgcact ctgcgcttca tcgctgacaa caactcggac
     1561 tactgcgacg ctctctccga tcctgaccat ccgaacgacc tgggggtgtt gtgggagcac
     1621 ctcacgcgac gcaaggactg gcacctgctg gatcttcgct atctgccgga agggtcacag
     1681 gtcgcgaccc tctcgcacgc tgccggacag cgcttctggt cactgcgaca gcgtctggat
     1741 gccgctccct acatcgatct gaggaccgac tggcgcgagc gcgtgcccag aaaccatcgc
     1801 accgaggtgc tgcgacggtg gcgccgtgtt caggagcagg gtcaggttac cttcgagatc
     1861 gcgcgcaccc ccgaggaagc ggacatgatg ctcgaccaac tggcccggat gcacattgcg
     1921 cgctggcagg gccgacatga gacgagcgtc ttccactttg ccgattaccg tcgctgggtg
     1981 catgacgtgt gccacgagct gctgaagcgc gaccagctct acctgtgccg gctctcgctg
     2041 gatgggaggc cgatgagcat ggcactggcg tttctgcaat cccgccggct gttgcgctat
     2101 acctcgacgt ttgacgaggc gtattcccgt ttcggccccg tgcatctgct cttgatggcc
     2161 gtggtggaag acttgcgggc gaacaacata gcggatgtgc atgaattcgg gcggggaggg
     2221 gaggcataca aggcacgttg gacacaccaa gccca
//
LOCUS       DAOSCV010000772         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31502_length_2255_cov_2.333182, whole genome shotgun sequence.
ACCESSION   DAOSCV010000772 DAOSCV010000000
VERSION     DAOSCV010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31502_length_2255_cov_2.333182"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2255)
                     /locus_tag="PLY56_16045"
     CDS             complement(<1..>2255)
                     /locus_tag="PLY56_16045"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOJ23040.1"
                     /translation="LVRAAVEEAAECGFQIWLYDEDNWPSGTAGGAVTAGHPEYRARY
                     LQLSDALAEQSSVTLSPEGEFLGAWAIPVDPEGEASGDAVPLSPGDDGRIHFDPPDGR
                     WRVCVFTVGDGGYVDLLNPEATERFLHLTHDRYAEAVGEHFGRCVPGIFMDEATLISI
                     WDVRDPRALPWSPRLPREFQVRCGYDLREHLPALVAAVPGAARVRCDYFSTLGALYAE
                     GFLKPVQAWCAAHGIASTGHLLCEEPLAPQVRHQANAPLAYRCFDIPGVDNLTVRTGG
                     LHHRLAASIAHQSHRARVLSETFGAAGWGTTLAQRKASADWQLAQGINLFVPHAVYYT
                     VYGKRKRENPPSEFEQEPFWPHYGRFSEYLARLSFLLTRGKHAARIAVLYPLRSAWAH
                     YHVGYQTPTGEEARVSFACYGSSDALLLARLESDLAALCTLLQELHFDFDFIDEAGLA
                     EARIEQSRLCLGPERYELLILPSMTTCARSTWARIREFFDEGGNILSCGLLPFQTGMG
                     EEEDQQLRREVQELTTLDPTAPDRPGGSTARAMGREAMRPLNVNREHGSRFARYLPWR
                     VPDPGQRSLLMKTLLRSLVTLDVDIDCPDLVCYQRDTADGKLFFLVNTADEERRVTAT
                     FYALGRPEEWDPETGEVRRVWQYARAGEKVTLPLHFAPRQARVLSFSGTEDLHVERAN
                     FVVTRVTETDEHYLVEGHARDYARPMETPPNCSLAWEGGSRWAEGIDRGLLEPITLGE
                     VWVLRVLGDNY"
BASE COUNT          387 a          754 c          781 g          333 t
ORIGIN      
        1 gtagttatcg cccagaacgc ggaggaccca cacctcgccc agggtaatcg gctccagcag
       61 ccccctatcg atcccctcgg cccagcggct acccccttcc caggcgagcg agcagttcgg
      121 cggcgtctcc attggccggg cgtagtcgcg cgcgtgccct tccaccaagt aatgctcatc
      181 ggtttcggtg acccgggtaa ccacgaagtt tgcgcgctcg acgtgcagat cctccgtgcc
      241 ggagaaggag aggacgcgcg cctgtctcgg ggcgaaatgg agcgggaggg tgaccttctc
      301 gcccgcgcgg gcatattgcc agacgcggcg cacctcgccg gtttccgggt cccactcctc
      361 cgggcggccc agcgcgtaga aggtcgcggt cacgcggcgc tcctcatccg cagtgttcac
      421 caggaagaag agcttgccat cggcggtatc acgctggtag cataccaggt cggggcaatc
      481 gatatcgaca tcgagggtga cgagcgagcg caggagcgtc ttcatcagca gggagcgctg
      541 gcccggatcc ggcacgcgcc acggcaggta gcgcgcaaac cgactgccat gctcgcggtt
      601 gacgttaagg ggccgcatcg cctcgcggcc catggcacgc gccgtgctcc cgcccggccg
      661 gtccggcgcc gtcggatcga gggtggtcag ctcctgcacc tcgcgccgca gctgctggtc
      721 ttcctcctcc cccatgcccg tctggaacgg gagaaggcca cacgagagga tgttgccgcc
      781 ctcatcgaag aactcgcgga tgcgcgccca cgtggagcgc gcgcacgtcg tcatcgatgg
      841 gaggatgagg agctcgtagc gctcaggacc caggcagagc cggctctgct cgatgcgcgc
      901 ttccgccagc cccgcctcgt cgatgaagtc gaagtcgaaa tggagctcct ggagcagagt
      961 gcagagcgcc gcgagatcgc tctccaggcg cgccaggaga agcgcgtcgg acgagccgta
     1021 gcacgcgaag gagacgcggg cttcctcgcc ggtgggagtc tgatagccga cgtggtagtg
     1081 cgcccaggcg ctccgcaaag ggtacaggac ggcgatccgg gcggcgtgtt tcccgcgcgt
     1141 caggaggaag gagaggcgcg ccagatactc cgaaaaccgc ccgtagtgcg gccagaacgg
     1201 ctcctgctcg aactccgagg gcggattctc gcgctttcgc ttgccataga cggtgtagta
     1261 gaccgcgtgc ggaacgaaaa gattgatgcc ctgcgccagc tgccagtcgg cgctcgcctt
     1321 tcgctgggcc agggtggtgc cccagcccgc ggcgccaaac gtctcggaga ggacacgcgc
     1381 ccgatggctc tggtgggcga tagaagccgc caaacggtgg tgcagcccgc cggtgcgcac
     1441 cgtcaggtta tcgacgccag ggatatcgaa gcagcggtaa gccagcggag cgttcgcctg
     1501 gtggcgcacc tggggtgcca ggggctcctc gcacaggagg tgcccggtag aagcgatgcc
     1561 gtgtgccgcg caccatgcct ggaccggctt caggaaaccc tccgcgtaga gcgcgccaag
     1621 ggtagagaag tagtcgcagc ggacgcgcgc cgcgcccggc accgcggcca ccagcgccgg
     1681 aaggtgctcg cgcaggtcgt agccgcatcg gacctgaaac tctcggggaa gacggggcga
     1741 ccagggcagc gcgcgcggat cgcgcacgtc ccagatgctg atgagcgtcg cctcatccat
     1801 gaagatgccg ggcacgcacc gtccaaaatg ctcgcccacg gcctcggcat agcggtcgtg
     1861 cgtgaggtgg aggaagcgct cggtggcctc cgggttgagg agatcgacgt agcccccatc
     1921 ccccacggtg aagacgcaga cgcgccagcg cccatccggc gggtcgaagt ggatgcggcc
     1981 atcgtcaccg ggcgagagcg gcacggcatc gccggatgcc tctccctccg gatcgactgg
     2041 gatcgcccat gcaccaagga actccccctc gggtgagagc gtgacgctcg actgctcggc
     2101 gagagcgtcg cttagctgca ggtagcgggc gcggtactcc ggatggcccg cggtgacggc
     2161 gccgcctgct gttccgctgg gccagttgtc ctcgtcgtac agccagatct ggaagccaca
     2221 ctccgcggct tcctcgactg cggcacggac cagcg
//
LOCUS       DAOSCV010000773         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31551_length_2252_cov_2.933091, whole genome shotgun sequence.
ACCESSION   DAOSCV010000773 DAOSCV010000000
VERSION     DAOSCV010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31551_length_2252_cov_2.933091"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PLY56_16050"
     CDS             complement(<1..1633)
                     /locus_tag="PLY56_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23041.1"
                     /translation="MAMRLLMLITLTLCLTSLCWGDATLRLGRATLTLDDSGRVISLL
                     PEGGADLASPYAPPAFKVTTAEGTLVPTSVTRQGKELLIRFGEQGHMRLAVTEGSGFA
                     WLKVTELSVPGTVERLQLFCLPVKGLETVASTINACYNERFATAVMATEINVRARPVS
                     RRAGDGNHQGCSHTFEPVTDSVRQGKTAARYSATSERGDNAGWTFVSRSFPMPLDLRG
                     CRAIRVWVYGDGGGQQLKIQLGDNRNGYRDDYIPIDFTGWKQVVCDGPSLNTLHYDSV
                     TRIGFYYNGLPAGKSVSCLIDQVEAVIGEGEEERVVTLEDFEDPGSDLWPFEGARLFA
                     ETEKRFGIQPAGVGIIACPRPEFEATIERFERASGLPSPHLGGVWGKRSPWIKRSYLF
                     ITRFKESDTDDVIAFAKRGRFDMILIDQGSWCASTGHYPINTRNFPRGLDSLRDTVAR
                     FKKAGFKVGLHYLAPSIYPPDPYLTPVPDPRLVKDAHAVLAGDVDEKADFIPTTAPPE
                     GFPAEDGGYRGSGAVIQIGDELIQYRERSMEPPYGF"
     gene            complement(1846..>2252)
                     /locus_tag="PLY56_16055"
     CDS             complement(1846..>2252)
                     /locus_tag="PLY56_16055"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HOJ23042.1"
                     /translation="IRHPENDNGILLWHAGAPLSMIHPDERVRLGKHWILPSPLSGMT
                     HFRLKEGPLTVARFDGDRGEYCLAVGEGHSIPGPETLNNYVWMEVDEWPRWERTLMEG
                     PFIHHIAMGYGHFGRALLEACRFVPGLKPVAL"
BASE COUNT          387 a          744 c          751 g          370 t
ORIGIN      
        1 ggaatccgta gggcggctcc atcgagcgct cgcgatattg gatcagctcg tcgccgatct
       61 gaatgaccgc gcccgagccc cggtagccgc catcctcggc gggaaagccc tccggcggcg
      121 cggtggtcgg gatgaaatcg gccttctcat caacatcgcc ggccagaacc gcgtgcgcat
      181 ccttcacgag gcgtggatca ggcaccgggg tgaggtaggg atccggcgga tagatggagg
      241 gcgccaggta gtgaagcccc accttgaacc cggctttctt gaaccgggcg acggtatcgc
      301 gcagtgaatc gagcccacgc gggaagttgc gcgtgttgat cgggtagtga ccggtggatg
      361 cgcaccagga gccctggtcg atcaggatca tatcaaagcg cccgcgcttt gcgaaggcga
      421 tgacatcatc ggtgtccgac tctttgaagc gcgtgatgaa taggtaggag cgcttgatcc
      481 agggcgagcg ctttccccac acgccaccca ggtgagggct gggcaagccg gacgcgcgct
      541 cgaagcgttc gatggtcgcc tcgaactccg ggcgggggca ggcgatgatc ccgacgcccg
      601 ccggctggat gccaaaccgc ttctctgtct ccgcgaagag gcgcgctccc tcgaacggcc
      661 acagatccga gccgggatcc tcaaagtcct ccagagtgac cacgcgctct tcctcgccct
      721 cgcctatcac cgcctccacc tggtcgatca ggcacgagac gctctttccg gcgggcaacc
      781 cgttgtagta gaaaccgatc cgggtgacgc tatcgtagtg gagcgtgttc agggagggcc
      841 cgtcgcagac cacttgtttc cagccggtga agtcgatggg gatatagtcg tcgcggtagc
      901 cgttgcggtt gtcgccgagc tggatcttga gctgctgtcc gccgccatcg ccatacaccc
      961 agacgcgaat ggcccggcac ccgcggaggt ccagcggcat tgggaagctg cgcgagacga
     1021 aggtccaccc ggcgttatcc ccgcgctcgc tggtcgccga ataccgcgcc gccgtcttgc
     1081 cctggcgcac ggaatcggtt accggctcga aggtatggct gcacccctgg tggttcccgt
     1141 cgcccgcccg gcgcgatacg ggacgcgcgc gaacattgat ctccgtggcc atcactgccg
     1201 tagcgaaacg ctcgttgtag caggcgttga tcgtcgaggc cacggtctcg aggcccttca
     1261 ccggcaggca gaagagctgg aggcgctcca ccgtgcccgg taccgaaagc tcggtgactt
     1321 tcagccaggc aaacccggat ccctccgtca ccgccaggcg catgtggccc tgctcgccga
     1381 agcgaatgag cagctccttg ccctggcgcg tcaccgaggt cggcacgagc gtgccctcgg
     1441 ctgtggtgac cttgaacgca gggggcgcgt agggagaggc gagatccgcc ccgccttccg
     1501 ggagcaacga gatgacccgg ccgctgtcat ccagggtcag cgtcgcgcgg cccaaccgga
     1561 gcgtggcgtc gccccagcag agcgacgtca agcagagagt gagggtgatc agcatcagca
     1621 atcgcatcgc catcagcaaa cctcctggtg tggactcgtg gctgggagcc agcgtgtccc
     1681 cctattggct gccggacccc gctctcctct cccgggcgag aagcaaccgg aggagcggtg
     1741 ccagcgcctg caatccgagg ttacttaccg agggcgaccg cgtattctcg cgaggagcct
     1801 gccggcgcgc gtaaccagcc ggccatgctg ggcggcgacg ggcgtctaca gcgccaccgg
     1861 cttcaggccc ggcacgaatc ggcacgcttc cagaagcgcg cgcccgaagt ggccgtagcc
     1921 catcgcgatg tgatggatga acggcccctc catgagcgtg cgctcccagc gcggccactc
     1981 gtccacctcc atccagacgt agttgttcag cgtctcgggt ccggggatcg agtggccctc
     2041 gccaacagcc aggcagtact cgccacggtc tccgtcaaac cgtgccaccg tgagcgggcc
     2101 ctccttcagc cggaagtgcg tcatgccgga aagcggactg ggcaggatcc agtgcttgcc
     2161 caggcgcacg cgctcgtcgg ggtggatcat cgagaggggc gcgcccgcgt gccagaggag
     2221 gatgccgttg tcgttctcgg ggtgccggat gg
//
LOCUS       DAOSCV010000774         2250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31578_length_2250_cov_3.340319, whole genome shotgun sequence.
ACCESSION   DAOSCV010000774 DAOSCV010000000
VERSION     DAOSCV010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31578_length_2250_cov_3.340319"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..533
                     /locus_tag="PLY56_16060"
     CDS             150..533
                     /locus_tag="PLY56_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ23043.1"
                     /translation="MPTRRILVVEDELNVRLTLAQMLRLSGYEVLEAATGDDAIALAR
                     GTNLDLLLSDIRLPGIDGYELLRRIRVLHPEIRGVAMTGYADGASQRLALEAGFDCLL
                     RKPFPFNVLLQAIASCLGSPPAGNA"
     gene            complement(777..1076)
                     /locus_tag="PLY56_16065"
     CDS             complement(777..1076)
                     /locus_tag="PLY56_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23044.1"
                     /translation="MLSDHSSQVNETLISIAATMRDLVDASIREGMLRAVPDPVEARQ
                     LADVHARLLHRIRAMEVALREFDADLRRADELFDRAERRMNGPNFLVEEAELIPQ"
     gene            1818..>2250
                     /locus_tag="PLY56_16070"
     CDS             1818..>2250
                     /locus_tag="PLY56_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23045.1"
                     /translation="MACALAIVLPGAARAEQTPAELLADLEAEGFENLSVEKISDGQG
                     VVITFENRRYRWEVVGLGVALGLATAHQEGRVILVPMHTGVPMGRIEVDASDYRAFLR
                     GELSEEEIRRGVAIATRTGRIVPVLCGSAYENVGVRALLDAI"
BASE COUNT          370 a          711 c          774 g          395 t
ORIGIN      
        1 ccccgcgacg ttatgcagat ccctcgggcc cacgaaccgg gcgatctggt tgtggtcgta
       61 tcggccggcg ctggtgtggc gagtaagggt agagctccct cacccacccc gcatcatggg
      121 agcgcccata ggggaatcga agacaagaga tgcccaccag gcgaatcctg gtcgttgagg
      181 acgaactaaa tgttcggctc accctggcac agatgctgcg gctttcgggc tacgaggtgc
      241 tcgaagcggc caccggagat gacgcgatcg cgctggcgcg cggtacaaac ctcgatctct
      301 tgctgagcga tatccgtctc ccagggattg atggctacga gctgctccgg aggatccgcg
      361 tgctgcaccc ggagatccgc ggcgtggcga tgacgggtta cgcggatggc gcttctcagc
      421 gcctggccct ggaggcgggc ttcgattgcc tcctccgcaa gcccttcccc ttcaacgtgc
      481 tgcttcaggc catcgcgtcg tgcctgggct cgcctcccgc gggcaatgcg tgagccgggc
      541 cggagagcgg cactggcgcg atcagggagg caaagctcga cagtcagacg cgaggcagga
      601 agcgggtggg gaacacggcg gcggggtgcc ccggagcgtc gcgccggggg cccggcgctg
      661 ccatcccgca tcgcccgcac gccagcgtgt ctggccggac cctaagggat gcacagaggc
      721 cccgggcctg gcggcgtgcc tgcctgtcgg cagcccttcc tggcggaaac gctcgtctat
      781 tggggaatca gctcggcttc ctccaccaga aagttgggac cattcatgcg gcgttcggcg
      841 cgatcgaaca gctcgtcggc gcgccgcaag tcagcgtcga actcgcggag tgcaacctcc
      901 atcgcgcgaa tgcggtggag caggcgggcg tgcacgtcgg caagctgcct cgcctcgacc
      961 ggatccggga cggcacggag catcccctcc cggatcgagg catctaccag gtctcgcatg
     1021 gttgccgcga tggagatcag tgtctcattc acctggctgc tgtgatcaga tagcatacgt
     1081 ccctccctca atttccccgg gtcctcccga gatgggcacc tgtcaggcat ctgctgtgcc
     1141 attcttcccc ctcccaagtg gggctaaacg cccgtcatgg ctcatgggat gcctcctggc
     1201 aggcccggca gctcgtgtca gcgggcgcca gagccgttcg ccggcaaggg caatgcctgg
     1261 gtgcgcccgg ccaatgcccc ctggataaga cgtccggtgg ccgtgcccgg cctccctgga
     1321 gagccccgca ggcccgctcc tttccttgac agggcaacag cctctgtgct atactcgcgc
     1381 caaccaggtg ggcggctgtc actcgcgagc gcggctgggt gccgtgcccg tcaccgctcc
     1441 tttcagggcc aggagtggcg ctgcgagcaa ggaggtgctc ttcggacagc cggcccgacc
     1501 ccgatcagag tccacaacgg ataccaccgc tgtcggatgg taacggaaaa cccgtctagc
     1561 gagaatagaa atccagtgag agcagccgct cgtacccttg acaccttttt gtggggtatg
     1621 ctatactacc gcaagcgtgt agggggtagg ggtggcttgc aggcagggag aggcctcaag
     1681 ccatcaagat gttcatgacg tcgcgtcttc acattgcgcc gggcctgcgg ggccggcgga
     1741 gttcggaacc cgcagcgggc catcgccgcg cttatcacaa gctcccgaag cgccgaaggt
     1801 ggacctggat aaccgcgttg gcgtgcgctc tggccattgt tctgccaggt gccgcgcgcg
     1861 ccgaacagac gccagccgag ttgctggccg atctggaagc ggaaggtttt gagaatctga
     1921 gcgtcgagaa gatctcggac ggccagggcg tggtgatcac gttcgagaac cgccgctacc
     1981 ggtgggaggt ggttggtctg ggggttgccc tgggccttgc gactgcccac caggaggggc
     2041 gggtgatcct ggtgccgatg cacacggggg tgccgatggg gcgcatcgag gtggatgcct
     2101 ctgattaccg cgccttcctc cgtggcgagc tgagcgagga agagatccgg cgtggcgtgg
     2161 cgattgcgac gcggaccgga cgtattgtgc ccgtgctgtg cggctcggcg tacgagaacg
     2221 tgggcgtgcg cgccctattg gatgcgatcg
//
LOCUS       DAOSCV010000775         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31711_length_2243_cov_2.277879, whole genome shotgun sequence.
ACCESSION   DAOSCV010000775 DAOSCV010000000
VERSION     DAOSCV010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31711_length_2243_cov_2.277879"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="PLY56_16075"
     CDS             <1..1585
                     /locus_tag="PLY56_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23046.1"
                     /translation="LLVVSESEVFPGVADLDPYPGMMAFALGQKTLPVFGTAAPHLPP
                     EVRALWAEGLRRVVDRCLPDDLVSARNQSSHYLVAYHAYATGSGDPLYRDMARLFARR
                     WIRGQHPSGFHQEATGPCPSYIGMTHWHEAVYYRMSKDPEILESLRRSYAFFNHTVAP
                     EPDGRMLGGFNFAHRVGEGFYLEQYGGARGILDDVLPEVGLWAGSAPSPEEEAKQRAD
                     AVQHVERFLAEPTLPRYPDIGTPRYLYHAEPDRSATWPAREPGSFVRNLSNELVAVKR
                     PGYYAVIYVGKPAAGEFYIRKREDLRLPFPGGIEENGGVLPEVRNATPFLGGGLTGFW
                     TPEYGHSVMAAAWSPTTHHGVMLTAPDGKRYWEDYFAHTFELDEAAGTLVISGRIEGW
                     PLAYRRAYRFGDDALEVVLTLTAEKEMAARSLVENIPFARGGWKARGATLSAGEKHAG
                     EASSDRLTVTDSTGAGIEIRFDQPRALLLVPEGLKAGGWRDLQFGRAEVRLPEKLAAG
                     QTIELRYTIRPIAAPKPTG"
     gene            1671..>2243
                     /locus_tag="PLY56_16080"
     CDS             1671..>2243
                     /locus_tag="PLY56_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ23047.1"
                     /translation="MASREIVQMSLAFAGPPRIAYGMGGGFPSDIAFVRRSPAPNSRA
                     TPWTQYDGYWEMHDEWGNTWRRLENVTKGEVWKGALEESWDLLESYEFPQTDAVELYE
                     GTAARCRELHEKGLFVLGSIGWPFTIARYLRRMEIFLADVAAEPERVSDLLWQITRHL
                     EKEIHRYADAGVDGIMTAEDWGTQDRLLVSP"
BASE COUNT          353 a          722 c          790 g          378 t
ORIGIN      
        1 cctcctggtc gtatccgaaa gcgaggtgtt tcctggcgtg gcggacctgg atccctatcc
       61 ggggatgatg gcgtttgccc tcgggcagaa aacgctccct gtattcggca cggcggcgcc
      121 ccacctcccc cccgaggtgc gcgccttgtg ggccgagggg ctgcgccgcg tggtcgaccg
      181 gtgcctgcca gatgatctgg tcagcgcgcg taaccaatcc tcgcattacc tggtggccta
      241 ccacgcctat gcgaccggga gtggcgatcc cctctatcgc gacatggcgc ggcttttcgc
      301 ccggcgctgg attcgtggcc aacacccctc gggcttccat caggaggcga cgggcccctg
      361 ccccagctac atcgggatga cgcactggca cgaggccgtc tactaccgca tgagcaagga
      421 cccggagatc ctggagagcc tgcgccgctc ctacgctttc ttcaaccaca cggtggcccc
      481 ggagccggat ggccggatgc tcggcgggtt caacttcgcc caccgcgttg gcgaaggctt
      541 ctatctcgag cagtatggcg gtgctcgggg gattctggat gacgtgctgc ccgaagtagg
      601 tctctgggcc ggatcggcgc cttcccccga ggaggaggcg aagcagcgcg cggacgcggt
      661 gcaacatgtg gagcgcttcc tggcggagcc cacgctcccg cgctatcccg atatcggcac
      721 gccgcgttac ctttatcatg cagagccgga ccgctcggcc acctggccgg cgcgcgagcc
      781 cggctccttc gtgcgcaatc tgagcaacga gctggtcgcc gtgaagcggc ccggctacta
      841 cgccgtgatc tatgtaggga agccggccgc cggcgagttc tacattcgca agcgcgagga
      901 tctgcgtctc cccttccccg gcgggatcga ggagaacggc ggcgtgcttc ccgaggtgcg
      961 caacgccacg ccgtttctgg gcgggggcct gaccggcttc tggacgccag agtatggcca
     1021 ctcggtgatg gccgccgcct ggtcgcccac cacgcaccac ggcgtgatgc tcaccgcgcc
     1081 ggatgggaag cgctactggg aggattactt cgcgcacacc ttcgagctgg acgaggccgc
     1141 aggcaccctc gtgatttccg ggcggatcga ggggtggccg ctcgcctatc gccgggctta
     1201 ccggtttggc gacgacgcgc tggaagtcgt gctcacactc accgcggaga aggagatggc
     1261 ggcgcggtcg ctggtggaga atatcccctt cgctcgcggc gggtggaagg ccaggggcgc
     1321 cacgctctcg gccggggaaa agcacgcggg cgaggcgagc tcggatcggc tcacggtgac
     1381 cgactccacc ggcgccggga tcgagatccg gtttgaccag ccgcgcgcgc tgctcctggt
     1441 gcccgagggt ctgaaggcag gtggctggcg cgacctccag ttcgggcgcg cggaggtgcg
     1501 cctcccggag aagctggctg ccggccagac tatcgaactc cgctatacca tccgcccgat
     1561 cgctgcgccc aaacccacgg gctagtgccc ggaggctttg gggagcgcgc ggagataggg
     1621 gctggacggg gagcgttctg tacgcccggg ccttcaaaga gaggggatcg atggcgagtc
     1681 gcgagattgt gcaaatgagt ctggcgttcg cggggccgcc tcggatcgcg tatgggatgg
     1741 gtggcggctt tcccagcgat attgcctttg tccggcggag tcccgcgccc aactcgcgcg
     1801 ccacgccctg gacgcagtat gacggctact gggagatgca tgacgagtgg ggcaatacct
     1861 ggcggcgcct ggagaatgtc accaagggcg aggtgtggaa gggtgcgctg gaagagagct
     1921 gggacctgct ggagagctac gagttcccgc agaccgatgc cgtggagcta tacgagggca
     1981 cggcggcgcg ctgccgtgag ctgcatgaga agggcctctt tgtcctcggg agcatcggct
     2041 ggcccttcac catcgcgcgt tacctgcgga ggatggagat cttcctcgcc gatgtggccg
     2101 ccgagcccga gcgcgtgagc gaccttcttt ggcagatcac ccgccacctc gaaaaggaga
     2161 tccaccgcta cgcggacgcg ggtgtggatg gcatcatgac cgccgaggat tggggcacgc
     2221 aggaccgcct cctggtgagc ccg
//
LOCUS       DAOSCV010000776         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31748_length_2241_cov_4.101098, whole genome shotgun sequence.
ACCESSION   DAOSCV010000776 DAOSCV010000000
VERSION     DAOSCV010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31748_length_2241_cov_4.101098"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLY56_16085"
     CDS             complement(<1..1156)
                     /locus_tag="PLY56_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ23048.1"
                     /translation="MRTLILSITFMLLLAGGLGPDARGARGAGNRNSRQAPQAEELSR
                     RAMELLTRIHRAQEAGPGTSARAFEANIPYALVQVEKAQLHLASAWGGTDKAAEALED
                     AERALRLLAEGKEIHDGAAGLVERAYLSPTDGSAQPYWVYVPKAKPPKEGWPLLVYLH
                     GYVPSTSKIDPWLPTEEFWELSEEKGFAVAVPHGRKNTDFEGIGEQDVLTVIEETKRH
                     YPIDPDRVFMGGPSMGGYGTYVIGLHHPHLFAGLVPMCGRSIPYVVEERYPDNLPEFK
                     RIAVDPNVPITLAENAKNLPVYLQQGERDYLTNPAHSRRLAAKWRQLGCPITYEEWRD
                     HDHYIYWKREAHAKAYDWAKKLRRNSEPREVVYTTFTPKYRRAYWVEIEGI"
     gene            1387..>2241
                     /locus_tag="PLY56_16090"
     CDS             1387..>2241
                     /locus_tag="PLY56_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOJ23049.1"
                     /translation="MAEQQRREMALSPFWLQASIITFVVGFTILGILAYVIYRDQPPI
                     PERVVNPAGEVLFTGDDIRRGQDVFQKYGLMQYGTLYGHGAYLGPDFTADYLHRQALL
                     MLEVYRDDPAASERVRRELRRNTYDPASKSVSFTEGQAEAYRRLMPVYAEAFRNPRAV
                     GPVPAVYIRDAEEVRQLTAFFAWAAWTTTAERPGKPYSYTNNWPPEPLAGNFPTADAL
                     LWSALSIIALLGGTGLILYLFGRYDWLGWRADGDGGWRVRPPGEVAITPGQRATRWYF
                     LVVGVLFLL"
BASE COUNT          338 a          765 c          743 g          395 t
ORIGIN      
        1 cgatcccctc gatctccacc cagtaggcgc ggcggtactt gggcgtgaac gtggtgtaga
       61 ccacctcgcg cggctccgag ttgcgccgga gcttcttcgc ccaatcgtag gccttggcgt
      121 gcgcctcgcg cttccagtag atgtagtggt cgtggtcgcg ccactcctcg taggtgatgg
      181 gacagccgag ctgtctccac ttcgcggcga ggcggcgtga gtgcgccggg ttggtgaggt
      241 agtcgcgctc gccctgttgc aggtagaccg gtaggttctt cgcgttttcc gcgagcgtga
      301 tcggcacgtt cggatccacc gcgatccgct tgaactccgg caggttgtcg ggatagcgct
      361 cttccaccac gtaggggatg ctccgcccgc acatcggcac caggcccgcg aagagatgcg
      421 ggtggtgcaa tccgatcacg taggtgccgt aaccgcccat cgacggcccg cccatgaaga
      481 cccggtccgg gtcgatcgga taatggcgct tcgtctcctc gatcacggtc agcacatctt
      541 gctcgccaat cccctcgaaa tcggtgttct tccgcccgtg gggcacagcg acggcgaagc
      601 ccttctcctc cgagagctcc cagaactcct cggtagggag ccaggggtcg atcttgctcg
      661 tgctcggcac gtagccgtgg aggtagacca gcagcggcca tccctccttg ggcggcttcg
      721 ccttgggaac gtagacccag tagggctggg ctgagccatc cgtgggcgag aggtaggcgc
      781 gctcgaccaa gccggcagcg ccgtcgtgga tctccttgcc ctcagcaagg agcctgagcg
      841 cgcgctccgc atcctcgagc gcctcggccg ccttatcggt tccgccccac gccgaggcca
      901 ggtgcaactg cgccttttcc acctgcacca gcgcgtaggg gatgttcgct tcaaacgcgc
      961 gcgcgctggt gcctgggccg gcctcctgcg cccggtggat ccgcgtgaga agctccatag
     1021 cgcgccggga gagctcctcc gcctgaggcg cctggcgtga gttccgattg cccgcccccc
     1081 gcgccccacg ggcatcgggc ccgagcccgc ccgcaagcag aagcatgaac gtgatggaga
     1141 gaatgagcgt tcgcatgctc ccgtggttcg gaggcaggcc gccgctttcc tgtttgccca
     1201 caagaccgca cccgggcagg cgtggggaag ccgcgtgttt agcaggcgcc cgatcaggga
     1261 agactccctc ctagccaggc aggcaggcgc ccctcgccaa gaggggcgag cacgaggcgt
     1321 gctgccggcg cgcctgcgct cccgcccggc gactctgtgt tcggctcccc tgaggaggaa
     1381 ctggcaatgg cagagcagca acggcgcgag atggcgctct ctcccttctg gctccaggca
     1441 tcgatcatca ccttcgtcgt gggcttcacc atccttggga tcctggccta cgtgatctac
     1501 cgcgaccagc cccctattcc cgagcgcgtg gtcaacccgg cgggcgaggt gctcttcacc
     1561 ggcgacgata ttcggagagg gcaggacgtc tttcagaagt atggcctgat gcagtacggc
     1621 acgctctacg ggcatggcgc ctaccttggt cccgatttca ccgcggacta cctgcaccgg
     1681 caggcgctct tgatgctgga ggtctatcgg gatgacccgg cggcgagcga gcgcgtgcgc
     1741 cgcgagctcc ggcgcaacac ctacgacccc gcgtccaagt ccgtgagctt cacggagggg
     1801 caggctgaag cctaccggcg gctcatgccc gtctatgccg aggcgttccg caacccgcgg
     1861 gcggtgggcc cggtgccggc ggtctatatc cgagacgcgg aagaggtgag acaactcacc
     1921 gccttcttcg cctgggccgc ctggacgacc accgccgagc gcccggggaa gccgtactcc
     1981 tacacgaaca actggccgcc ggagccgctg gcgggaaact tcccgaccgc cgacgcgctc
     2041 ctctggagcg cgctcagcat catcgcgctc cttggtggca ccggactcat cctctacctc
     2101 ttcgggcggt atgactggct ggggtggcgc gcggacggcg atggaggctg gcgcgtccgg
     2161 ccgcccggtg aggtggcaat cacgcccgga cagcgagcaa cgcgttggta cttcctggtg
     2221 gtgggcgtgc ttttcctgct g
//
LOCUS       DAOSCV010000777         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31750_length_2241_cov_3.706770, whole genome shotgun sequence.
ACCESSION   DAOSCV010000777 DAOSCV010000000
VERSION     DAOSCV010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31750_length_2241_cov_3.706770"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLY56_16095"
     CDS             complement(<1..629)
                     /locus_tag="PLY56_16095"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOJ23050.1"
                     /translation="MSDILTQDEITALLSAFNRAEGAELDPESGATYEIKIYDFKRPH
                     KFSKDQVRTLEMIHERYARLLSTTLSTYLRAQTQVTLTGVDQLSYDEFIRSVPNPTVL
                     NIFSFASEEGNAILEINPGIAYTVIDRMLGGKGAPPDRPRELTEIERALMTRIAGLAL
                     RDLGEAWSSLVDVTPTLHSISTSMLFSQIALPGDMVMLVTFEVELCGVT"
     gene            complement(723..1196)
                     /locus_tag="PLY56_16100"
     CDS             complement(723..1196)
                     /locus_tag="PLY56_16100"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOJ23051.1"
                     /translation="MKIEFIEPFLRAAFEVLEQVLEERPERGQLSMRNATFTSQQVTI
                     VHGVVGMVEGSVLYGMSLVTAEKIASIMIGRPLMSFDDLANSAISELGDMVTGNAATY
                     LSNAGYQCNITPPSVMRGANLRISTITPALVVPVNTVAGRLEINIALQERKSPAG"
     gene            complement(1345..1707)
                     /locus_tag="PLY56_16105"
     CDS             complement(1345..1707)
                     /locus_tag="PLY56_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014476812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ23052.1"
                     /translation="MAIRVMVVDDALFMRTRLREILTEAGFDVAAEAENGSQAVERYP
                     AVKPDVVVMDMTMPVMDGLTALKEIRALDPDARVVLCSAMGQQRLVMEATQAGVRDYI
                     TKPFQAQRVVDGVIRAAS"
BASE COUNT          396 a          785 c          644 g          416 t
ORIGIN      
        1 ctggtcacgc cgcacagctc cacctcaaag gtcaccagca tgaccatatc gcccgggagg
       61 gcaatctggc tgaagagcat gctggtggag atgctgtgca gcgttggcgt cacatccacg
      121 aggctgctcc aggcctcgcc caggtctcgc agcgccaggc cggcgatccg ggtcatcagc
      181 gcgcgctcga tctccgtcag ctcgcggggc cggtcgggcg gggcgccctt cccaccgagc
      241 atccggtcga tcaccgtgta ggcaatgccc ggattgatct ccaggatcgc gttgccctcc
      301 tcagaggcaa aggagaaaat gttgagcacc gtggggttgg gcaccgagcg gatgaactca
      361 tcgtaggaga gttgatcgac gccggtgaga gtgacctgcg tctgagctcg caggtaggtc
      421 gagagcgtgg tgctcaagag gcgggcatac cgctcgtgga tcatctccag cgtccgcacc
      481 tggtccttgg agaacttgtg cgggcgtttg aagtcgtaga tcttgatctc gtatgtggcg
      541 ccgctttcag gatcgagctc ggcgccctcg gcacggttga atgccgagag caacgctgtg
      601 atctcatcct gtgtcaggat atcagacact tcggttcatc ctcaccctgg aaaggaggcg
      661 ggcgctggcg gatggcgatg ccccggggag gcgcctgccc agcgccagac tcccgcccag
      721 gcctaacccg caggcgactt gcgctcttgc agcgcgatgt tgatctccag ccgcccggct
      781 accgtattga ccggcaccac cagcgccggc gtaattgtgc tgatccgcag gttcgccccg
      841 cgcatcaccg aggggggcgt aatattgcac tggtaacctg cattcgatag gtaagtcgcc
      901 gcgttgccgg tcaccatgtc gcctagctcc gagatggcgc tgtttgccaa gtcatcaaaa
      961 ctcatcagcg gacggccaat catgatggat gcaatcttct cggcggtgac caacgacatg
     1021 ccgtagagca cggagccctc taccatgccg accacgccat ggacaatcgt cacctgctgc
     1081 gaggtgaagg tcgcgttgcg catcgagagc tggcctcgct ctgggcgctc ctccagcacc
     1141 tgctccaaca cctcgaacgc agcccgtaag aacggttcga taaactcgat cttcattccc
     1201 tgctcctgct gaagcgtggc gcccccccga tctccagccg cccgcgccac acacaccatc
     1261 ggcaacgacg cgcctcgcgg gaggtctcag gcacgcccgg gaacacccca cgacgatcca
     1321 ccgggatcgc tcgattctgc ctggctagct ggcggcccgg atcacgccat ccacgacgcg
     1381 ctgggcctgg aagggcttgg taatgtaatc gcgcaccccc gcctgcgtgg cctccatgac
     1441 gagacgctgc tgtcccatcg ccgagcagag aaccacccgg gcatctggat ccagcgcgcg
     1501 gatctctttc agagccgtga ggccatccat caccggcatg gtcatatcca tcacgaccac
     1561 atcgggcttc actgcaggat agcgctccac agcctggctg ccgttctccg cttcggcggc
     1621 cacgtcaaag ccggcttctg tcaaaatctc gcgcagtctg gtgcgcatga agagcgcgtc
     1681 atcgaccacc atcactctaa tcgccatggc tcctcccctc cccggtgggt agcatgagcc
     1741 gctccgccca gatccggccg tcctcaacag cgtatctccg caaacatcct gccgcacccg
     1801 cgactcaaca ccctgccatc cactccaccg ggaggggtga cgcttgcgat tcccgccagg
     1861 tccccctcaa ctcatccaat ccccttggca tgctcgcagc cggcaaccaa tgcattcatc
     1921 ggcgcaatgg gactatgcca catccctctg ccatccaccg gtcgcccgca gccgttgcct
     1981 gtccagaggc ggcgcgcgtc cagggtacct gcgatggccg agcggctcat ctccgtccgg
     2041 cagcctgggc tggcatgccc ggttacggat ctcaggctcc cccgttggtg attcggcatt
     2101 cgccgggcta accttaagct ggcgcgcctt ctgccacgcc tctgcgctct gcttccggcc
     2161 cacgcacacc ggtgcattct cccctccggc ttacctccac gcgggggtcc cataagcgtc
     2221 tttccagcag acacttgtgg c
//
LOCUS       DAOSCV010000778         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31777_length_2240_cov_2.891533, whole genome shotgun sequence.
ACCESSION   DAOSCV010000778 DAOSCV010000000
VERSION     DAOSCV010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31777_length_2240_cov_2.891533"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLY56_16110"
     CDS             <1..444
                     /locus_tag="PLY56_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23053.1"
                     /translation="VFFLVALVIFALMSASYFVAGMLTSGGSKGLIQEISQAAECGRN
                     HLLPTAAAIDRYARGTGKYPEQIDDLLPMYLEKQEVLRCPADTTTPKGESSFLYHRPQ
                     PGDTDAVLLECPHHNTLLMRVRARGKTEPGKTFWELQMQPLTQAP"
     gene            complement(441..1085)
                     /locus_tag="PLY56_16115"
     CDS             complement(441..1085)
                     /locus_tag="PLY56_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HOJ23054.1"
                     /translation="MIFEDRVDAGRRLAKALEEYRGRDLVILGVPRGGVVVADEVARA
                     LNAPLDIVVARKIPAPGNPELAIGAVAADGSDMIDPMARHYPGVTPEYLERAKAEQIA
                     EIQRRLQAYRGGRPAPDIAGKTVIVVDDGIATGSTVMASLRSLRNRGAAELILAVPVG
                     PPSSIERLRAEADRVVVLETPEPFFAVGEWYQRFDQVTDEEVVEILRRAGAPAR"
     gene            complement(1227..1595)
                     /locus_tag="PLY56_16120"
     CDS             complement(1227..1595)
                     /locus_tag="PLY56_16120"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOJ23055.1"
                     /translation="MFEVATRRHFDAAHCLRGYQGKCERLHGHRFEVEAVVRAEELDA
                     TGIALDFTLLKRLLGAICDEPDHRNLNELPPFQQENASSEGLARYIYRRLAPQIPEPA
                     RLLSVTVWESPESWARYSEP"
     gene            complement(1739..>2240)
                     /locus_tag="PLY56_16125"
     CDS             complement(1739..>2240)
                     /locus_tag="PLY56_16125"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOJ23056.1"
                     /translation="GTASIKNVLPQQFSLGFYYRETPELTLLAEYSWTEYSANRVLDV
                     TGNLGPTSLDTSGDITQNWCNVSNIRLGAEYTGFGPWALRAGYVYTTRATPSDQARAT
                     FSVPGHGHTFTLGVGRSLRERVEINAALEYSFASGRGSNAADGITEGSFRSDAVVLHT
                     GAMYRF"
BASE COUNT          385 a          779 c          703 g          373 t
ORIGIN      
        1 gtcttcttcc tggtggcact ggtgatcttc gctctcatgt ctgccagcta cttcgtggca
       61 gggatgctca ccagcggagg cagcaagggg ctcattcagg agatcagcca ggccgcggag
      121 tgcggccgaa accacctgct ccccacggct gccgccatcg accgctacgc tcgcggcacc
      181 gggaagtatc cggagcagat tgacgatctc ctccccatgt atctggagaa gcaagaggtg
      241 ttgcgctgcc cagccgacac cacgacgccc aagggggaga gcagcttcct ctaccaccgg
      301 cctcagccgg gtgataccga cgcggtgctg ctcgagtgcc cccaccacaa caccctcttg
      361 atgcgcgttc gcgcgcgcgg gaagaccgag ccaggcaaga ccttttggga gctgcagatg
      421 cagccgctga cccaggcgcc ttagcgggcg ggcgccccgg ctcgtctcag gatctcgacg
      481 acttcttcgt cggtaacctg gtcaaaccgc tggtaccact cacccacggc gaagaacggc
      541 tctggcgtct ccaggaccac cactcggtcg gcctcggccc ggaggcgctc gatgctggaa
      601 gggggtccga caggcacggc gaggatcagc tcggcggccc cccgattcct caggctccgc
      661 agcgaggcca tcaccgtgct tccggtggcg atgccatcat ccaccacgat caccgtcttg
      721 cccgcgatat ccggggccgg gcggccaccg cggtaggcct gtagccggcg ttgaatctcg
      781 gcaatctgct ccgccttcgc gcgctcgaga tactccgggg tgactccagg gtaatgcctg
      841 gccatggggt caatcatatc tgagccgtcc gcggccactg cgccaatggc gagctccgga
      901 ttccccggcg ccgggatctt gcgcgccacg acaatatcga gcggcgcgtt cagcgcccgc
      961 gccacctcat cggccacaac caccccgccg cggggaacgc cgaggatcac gaggtctcgg
     1021 ccccgatact cttccagggc tttcgccagc ctcctgccgg catccacccg atcctcgaag
     1081 atcatcgcgc tcacctccgt ctctgcctct tcccgcgctc cggttggcca aaacagcacg
     1141 cgagaggagc gccgcactcc gcgccgtggg cgatggcgcg ggcacggaga cgcgggcgcc
     1201 atcggattac ccgccctggg cggagctcag ggctcggagt accgtgccca ggattctggg
     1261 gactcccata cggtcaccga gagaagccgc gccggctctg ggatctgggg agccagccgg
     1321 cggtagatgt aacgcgcgag gccctccgag ctggcgttct cttgctggaa cggcggcaac
     1381 tcgttcaggt tgcgatgatc cggctcatca cagatggctc ccagaagacg cttcaggagg
     1441 gtgaaatcga gggcgatgcc ggtggcatcc aactcctccg cgcggaccac cgcctccacc
     1501 tcgaagcggt gcccgtgcag acgctcgcac ttgccctggt agccccgcag gcagtgcgcg
     1561 gcgtcaaagt gccggcgtgt cgcgacctca aacatcctct cgattcctcc cctccacgga
     1621 cggggcgagg ggtccggatc atgagatccg ccccctcacc tcggtccttc tattccccta
     1681 ttggttcaac actcaccggg cccaaaggcc cggtgcaacc ggagtgacat taccctactc
     1741 agaagcgata catcgccccg gtgtgcagga cgacggcgtc gctgcggaaa ctgccctcgg
     1801 tgattccatc ggccgcgttc gagccacgtc cggacgcgaa gctgtactcc agcgccgcgt
     1861 tgatctccac gcgctcccgg agcgagcggc cgacaccgag cgtgaaggtg tggccgtggc
     1921 ccggcacgga gaaggtcgcg cgcgcctgat ccgacggggt tgcgcgggtc gtatacacat
     1981 atccagcccg aagcgcccac ggtccgaagc cggtgtactc cgcgccaagg cgaatgttgc
     2041 tcacgttaca ccagttctgc gtgatatcgc cgctggtgtc gagcgaggtc ggtccaagat
     2101 tgccggtgac atccaggact cggttggccg aatactcagt ccaggagtac tccgccagga
     2161 gggtgagctc cggcgtctcg cggtagtaga agccgagcga gaactgctgc ggcagaacgt
     2221 tcttgatcga cgcggtgccg
//
LOCUS       DAOSCV010000779         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31881_length_2235_cov_2.952294, whole genome shotgun sequence.
ACCESSION   DAOSCV010000779 DAOSCV010000000
VERSION     DAOSCV010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31881_length_2235_cov_2.952294"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1105
                     /locus_tag="PLY56_16130"
     CDS             218..1105
                     /locus_tag="PLY56_16130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019006785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ23057.1"
                     /translation="MKYRTLGKTGMRVSVIGIGTWQFGGAWGKTFTEEEVAAILGRGR
                     ELGINLIDTAECYGENHRSETLIGHFLKGQPREEWIVATKFGHLPGWKPDEVRQQLED
                     SLRALQTDYVDLYQFHSGTDEEFDTPGLWEMLQEQVRLGKVRHLGISVPTPSTPEENL
                     YQVDSATRVGAEAIQVVYNRLHRIPEEFVFPSCERQNLGVLARVPLASGFLSGKYDAS
                     AHFPSNDVRSHRDPAQIRATVAEVERIRQQEVPEGVPMAQWALAWCLKNPAVTCVIPG
                     CKSIQQVEQNAAAADLVEP"
     gene            1214..>2235
                     /locus_tag="PLY56_16135"
     CDS             1214..>2235
                     /locus_tag="PLY56_16135"
                     /inference="COORDINATES: protein motif:HMM:NF037031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HOJ23058.1"
                     /translation="MALEVRTDFPGGNACAIETAALPERDVVRFAADPRGGTEALWFW
                     IRIEGCTGRPVELLLTNADTCLGFGAGGRTTRPVYRHASGDWERVTETEIRELPDGQH
                     EAVWVVNPRASSFDFAFCYPYLPEDLAWTLSECPGYWHEAAIGVTQKGRPLTHLANAF
                     GNEPAQAAGIYVIARQHAGETPGSWVLDGILRHARRAVDPARVLLWAVPFAHLDGVLE
                     GDYGKDPFPYDLNRAWTRPPMRHEVLVIQRDLHRWARRCRPALVVDLHGPGAAETEGA
                     YFHLPRPSRPEEHVDATRAAALFIRERLPAEFIREQPDVQATYASRWSDGGILSSYVW
                     DTLGIPS"
BASE COUNT          368 a          736 c          773 g          358 t
ORIGIN      
        1 cgcggatgac ctgttccata ggctcttatt gttgatgcgc gtcgcgatcc ccttcttgga
       61 tcgcgtctgt aagtgggccg gctaaacggt ggagcacgcc gggtaagctc atgtcagacc
      121 gatgcctttg ggggccccat ccaaacagga atgccccctg aacggcgcgt atctcctcca
      181 cggaggcatt gccggccggt ggatggaggg caggccagtg aagtaccgca cgctgggcaa
      241 gactgggatg cgcgtctcgg tcatcggcat cggcacgtgg cagttcggag gtgcctgggg
      301 aaagacgttc accgaggagg aggtggcggc catcctcggt cgcgggcgcg agctggggat
      361 caacctgatt gataccgccg aatgctatgg agagaaccac cgctccgaaa cgctcatcgg
      421 gcacttcttg aaggggcagc cccgcgagga gtggatcgtg gcgaccaagt tcggccacct
      481 gcccggatgg aagccggacg aggtgcgtca gcagctggag gactcgcttc gggcgctgca
      541 gacggactac gtcgacctct accagttcca ctccggcacc gacgaggagt tcgacacgcc
      601 ggggctgtgg gagatgcttc aggagcaggt gcgcctgggc aaggtgaggc acctcggcat
      661 ctccgtgccc accccaagca ctcccgagga gaacctgtac caggtggact ccgcaacgcg
      721 cgttggcgcc gaggcaatcc aggtggtgta taaccgcctc caccgcatcc ccgaggagtt
      781 cgtcttcccc tcgtgcgagc ggcagaacct gggtgtgctg gcgcgcgtgc cgctggcaag
      841 cggcttcctc agcgggaagt atgacgccag cgcccacttc ccgtccaacg atgtccgctc
      901 gcaccgtgac cctgcgcaga tccgcgcaac cgtggcggaa gtggagcgaa tccggcagca
      961 ggaggtgccc gagggcgtgc cgatggcgca gtgggctctg gcgtggtgcc tgaaaaaccc
     1021 ggccgtcacc tgcgtcattc ccgggtgcaa gagcatccag caggtggagc agaacgcggc
     1081 ggcggcggat ctggtggagc catgagcgcc gggcggaccc aatccgcccg gtcaccctgg
     1141 gtggtgaggc cggcttggcg gcgcggtcag ccgccggccc gcgtccgtcc ccacccccat
     1201 gagaggagcc accatggctt tggaagtaag aaccgatttc ccgggtggga acgcgtgtgc
     1261 tatcgaaacg gcggcgctgc ctgaacgtga cgtcgttcgc ttcgcggccg atccgcgcgg
     1321 tggaaccgag gcgctctggt tctggatccg catcgaggga tgcaccgggc gcccggtcga
     1381 gctgctcctc acgaatgccg atacctgcct cggtttcggc gcaggcggga gaacaacgcg
     1441 cccggtctat cgacacgcca gtggcgactg ggagcgggtg accgaaaccg agatccgcga
     1501 gctgccggat ggccagcatg aggccgtctg ggtagtgaac ccgcgggcgt cctccttcga
     1561 tttcgcgttc tgctacccct acctgccgga ggacctggcg tggacgctct ccgaatgccc
     1621 gggctactgg cacgaggccg cgattggcgt tacgcagaag ggacggccac tcactcacct
     1681 ggccaacgcg ttcggtaacg agcccgcgca agcggccggg atctacgtca tcgcccggca
     1741 gcacgccggt gagacgcctg gctcctgggt gctcgatggc atcctccggc acgcgcgccg
     1801 cgcggtcgat ccggcgcggg ttctcctctg ggcggtgccg ttcgcccacc tggatggcgt
     1861 gctggaaggc gactatggca aggatccctt cccctacgat ctcaaccggg catggacgcg
     1921 cccgccgatg cgccacgagg tcctcgtcat ccagagagac ctgcaccgct gggcgcgccg
     1981 ctgccgcccg gcgctggtgg tggatctgca cggccccggc gcggccgaga cagagggcgc
     2041 ctacttccac ctgccgcggc cctcacgccc cgaggaacac gtggatgcga cgcgcgcggc
     2101 ggcgctcttc atccgggagc gcctccccgc ggagttcatc cgcgagcagc cggacgtgca
     2161 ggcgacctac gcctctcggt ggagcgatgg aggcatcctg agcagttacg tgtgggacac
     2221 gctcggcatc ccgtc
//
LOCUS       DAOSCV010000780         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31925_length_2233_cov_3.251607, whole genome shotgun sequence.
ACCESSION   DAOSCV010000780 DAOSCV010000000
VERSION     DAOSCV010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31925_length_2233_cov_3.251607"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLY56_16140"
     CDS             <1..800
                     /locus_tag="PLY56_16140"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOJ23059.1"
                     /translation="RLMGGGSLVAGLAPRLGQLLGMPAGALELPALATVARADAAQYA
                     GAYGMALLAAGAAQTADLLAEEVRRAQHLRQRRRNAWMGAAVALVVLIGGCAAAHQRW
                     RDHLDLQRARAAAARELATERSRAKKLDARYQELTAQAESLRRALRPNPPWVDVMDAL
                     AMAAPQGVWVTGIELERGRPLVIRGTALEAAGAAQFTNALMESPLLEQVQLSFANGAQ
                     VGGRQVTHFGISAVVRGNTAERRVIAKASTARKRPASAKRAAEEEEE"
     gene            797..1321
                     /locus_tag="PLY56_16145"
     CDS             797..1321
                     /locus_tag="PLY56_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23060.1"
                     /translation="MKLSRREIAMVALCVVVVAFIGVPSLKQMQPVESRTPEALAADA
                     GRVRARCAALEQEIASLEKEVRSLSWSEEPSRLPTRILTELDLVAEKAGVTLATLRPG
                     RPVAVASGSKLPFTVQVRAPFPKAIRFLRRLQQTQDRVALERLQVVSTDASTDIVSLE
                     LRLAVYSSVPPESK"
     gene            1365..2189
                     /locus_tag="PLY56_16150"
     CDS             1365..2189
                     /locus_tag="PLY56_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23061.1"
                     /translation="MWVVLGLAGAGASALALTTPQDETRFVSVRPPAKRSVSKASQAT
                     AAPSGGKALLAPSASGSETTKAASSNGGGPEASGKSQAATAVKSGAAEERNLFRPLVV
                     AKSGRASDSLSLPPAGLDGLLPLPAGNPAEGGRQQRSYWQYVGTVRLDGVSYALVEEQ
                     KSKEGHYLKRGDLFRGGRVEVLNPDYLVISVDGKRHTLYKSSGMEETSTKGSAAVRQA
                     SASSAPVAQGQQTAAPEAHSHRRLPRPRARSRALREVRPRVLQALPPLRARRPLPS"
BASE COUNT          367 a          694 c          824 g          348 t
ORIGIN      
        1 tccgcctcat gggagggggc agtctggtgg ctggattggc gccgcgcctg ggccaactct
       61 tggggatgcc cgccggcgcc cttgaactcc ctgcgctcgc gacggtagcg cgggccgacg
      121 cggcgcagta tgctggggcc tatggcatgg cgttactcgc cgcgggggcc gcacagactg
      181 cggatctctt ggccgaggag gtgcggcgcg cgcagcactt gcgccagcga cggcgcaacg
      241 cctggatggg ggccgccgtg gccctcgtgg tgctgatcgg ggggtgcgcg gcggctcacc
      301 agcggtggcg tgaccatctc gacctccagc gggcacgggc agccgcggcg cgcgagctgg
      361 cgacagagcg ctcccgggca aagaagttgg atgccaggta ccaggagcta acggcgcaag
      421 cagaatcact gcgccgggct ctgcgcccga atccaccgtg ggtggatgtg atggacgcgc
      481 tggcgatggc agcgccgcag ggcgtctggg tgacgggaat cgaactggag cgaggccgac
      541 cgctcgtcat ccggggcact gcgcttgagg cagccggagc agcacagttt accaacgcgc
      601 tcatggagtc cccgctgctg gagcaggtcc agctcagctt tgcgaatggg gctcaggtgg
      661 gcggacggca ggtgacgcac tttgggatca gcgccgtggt gcgcggcaat accgccgagc
      721 gccgtgtcat tgcgaaagcg tctaccgcgc ggaagcgacc ggcctccgcg aagagggctg
      781 cggaggagga agaggaatga agctctcacg acgcgagatc gcgatggtgg cgctgtgcgt
      841 ggtggtggtg gccttcatcg gggtgccctc gctgaagcag atgcagcccg tcgaaagtcg
      901 cactcccgag gcgctcgccg cggatgctgg gcgcgtgcgc gcgcgctgcg ctgccttgga
      961 gcaggagatc gccagtctgg agaaggaggt gcgctcgctc tcgtggagcg aagagcccag
     1021 ccgcctgccg acacgtattc taaccgagtt ggatctcgtc gcggagaagg cgggggtgac
     1081 gctggcgacg cttcggcctg gccgcccggt ggccgtggcc agtgggagca agctcccctt
     1141 cacggtgcag gtgcgggcgc cctttccaaa agcgatccgg tttctgaggc gcctccagca
     1201 aacccaggat cgcgtggcgc tagagcgcct gcaggtggtg tctacggatg cgagcacgga
     1261 catagtcagt ctggaattga ggcttgccgt gtactccagc gtgccacccg agtcgaagtg
     1321 aggacgtgtt tgaggtaggt atgccagagc aacggagatg ggtgatgtgg gtggtcttgg
     1381 ggctcgccgg agcaggtgcg agtgccctgg ctcttacaac gccccaggat gagacccggt
     1441 tcgtgagcgt tcgccctccg gcgaagcggt cggtgagcaa agccagccag gcgacggcgg
     1501 ctcccagtgg aggcaaggcg ttgctggcgc cctccgcctc agggagcgag acgacaaagg
     1561 ccgcttcgtc caacggtggc ggcccggagg cttcggggaa gagccaggca gcaaccgccg
     1621 taaagagcgg tgcggcggag gaacgtaacc tcttccgccc gctggtcgtt gccaagtcag
     1681 ggcgcgcttc cgattcgctc tctttgccac ccgccggcct ggatggcctt cttccgttgc
     1741 ccgcaggcaa ccctgccgaa ggagggcgcc agcaacgctc ctactggcag tatgtcggca
     1801 cggttcgtct ggatggcgtc agctacgcgc tggtagagga gcagaaatca aaggagggcc
     1861 actacctgaa gcgtggcgat ctcttccgtg gcgggcgtgt ggaagtgctg aatcccgatt
     1921 acctggtcat ttcggtggat ggcaagcgcc acacgttgta caaatcctcg ggcatggagg
     1981 agacgtccac gaaggggagc gccgcagtgc gccaggcatc cgcttcttcg gcgcctgtgg
     2041 cccaaggcca gcaaacggcg gcgccggagg cgcacagcca ccgacggctc ccgcgcccca
     2101 gggcgcgcag ccgggcgctc cgggaagtcc gcccgcgagt cctccaggcg ctcccacccc
     2161 tccgggctcg acgcccactc ccgtcatgac aacgatggcg cccggcgccg tggcctcggg
     2221 gacgagccag aat
//
LOCUS       DAOSCV010000781         2229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_31989_length_2229_cov_3.488960, whole genome shotgun sequence.
ACCESSION   DAOSCV010000781 DAOSCV010000000
VERSION     DAOSCV010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31989_length_2229_cov_3.488960"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="PLY56_16155"
     CDS             <1..1477
                     /locus_tag="PLY56_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23062.1"
                     /translation="LSDAKTFTTVPFFLPSLPATALLFQATAFNSAGTAIAQGSRSAT
                     IDPLTEALVTVALVTSQPGGGDEPGTSKPPVTTGSAVLNITNIAQGDALVVVDPMPGD
                     PSLLGTAADRSPITVPLQGTFVSDNLPVTRVPVLVGGLEAGPWLFVVTTHTGPTTADP
                     VNRVGTVQGTVQAGALVNLDVPLGAAQTSTPPKPDGEVNVLATNITAAGTEFLLVTAF
                     DSLGRVREAVVPSMAGVTERQVTFSLPGGPYTIVCAAYGGPGFDRAAFDQALSENKTS
                     IPSGALAVGTKTVTVGSGAATDATVELRIQGETGAGSINLAMLNVPLNLRKSDAGYEI
                     VSSVVIRVYSVNTNQELSALRRVMVPPSFGVSEEDEDGESQPSVPGDKTLLDLSAPYH
                     MLKPMRLRIDGVPVGLMRFEVFAMSESGGGGQVMGSGSTIAEIGTGVQPVTVTLLPHG
                     NLEPGTVLPGIDNEPSDENPAPRSRSALEAARRFWLGLRRR"
     gene            1628..2017
                     /locus_tag="PLY56_16160"
     CDS             1628..2017
                     /locus_tag="PLY56_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ23063.1"
                     /translation="MPIYEYRCVGCNRKFSHLHGMVANEAPLQCPRCGGTELKRLISR
                     VSRVRSEEDVFDALDPSNFGDMEDPADVRRWARALGKEMGEELGEDFEESLDEMIEAE
                     ERGELSDEAGGEDAPASPAPAASSDDL"
     gene            2007..>2229
                     /locus_tag="PLY56_16165"
     CDS             2007..>2229
                     /locus_tag="PLY56_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23064.1"
                     /translation="MTSDTPSGRAAGLPHGEDAMGERPTSSDDRGAAIPGRVLAATFL
                     VALAGVCLEVALVRVFALLFRYHHLFLLVS"
BASE COUNT          348 a          692 c          809 g          380 t
ORIGIN      
        1 cctctcggat gccaagacgt tcacgaccgt gccattcttc ctcccctcgc tcccggccac
       61 ggcgctcctt ttccaggcga cggcgttcaa ctcggccggc acggccatcg cccagggctc
      121 gcgcagcgcg acgatcgatc ccctcacgga ggcgctggtg actgtggcgc tggtcacatc
      181 ccaaccggga gggggggatg agcccggtac gagcaagccg ccagtgacga cgggatctgc
      241 cgtgctcaac atcaccaaca tcgcgcaggg agatgcgcta gtggtggtgg accccatgcc
      301 gggtgatccc agcctgctgg ggaccgcggc ggaccgcagc ccgatcacgg ttccgcttca
      361 aggcacgttc gtaagcgata acctcccggt gacgcgcgtg ccggtgctgg tgggcggtct
      421 tgaggccggc ccttggctct tcgtggtcac gacgcacact gggccgacga ccgccgatcc
      481 ggtgaaccgc gtcggcaccg ttcaggggac tgttcaggcg ggcgcgcttg tgaacctcga
      541 tgtgcccctc ggcgccgccc agacgagcac gccgcccaag ccggacgggg aagtgaatgt
      601 cctcgccacc aacattaccg ctgcggggac cgagttcctc cttgtgacgg ccttcgactc
      661 cctggggcgt gtccgcgagg cggttgtccc aagtatggcc ggcgtgactg agcgccaggt
      721 caccttctcc ctgccgggcg ggccctatac gattgtctgc gccgcctatg gcggccccgg
      781 gtttgaccgt gccgcattcg atcaggcact gagtgagaat aagacgtcga ttccctcggg
      841 cgcgctcgcc gtgggaacga agacggtgac ggtgggcagc ggcgcggcga ccgacgcgac
      901 ggtcgagctg cgcattcagg gcgagacagg cgccggaagc atcaacctgg cgatgctgaa
      961 tgtgcctctg aacctgagga aaagcgacgc cgggtacgag atcgtctcca gcgtagtgat
     1021 ccgggtttac tcggtgaaca ccaaccagga gctgtccgcg ttgcggcggg tgatggtgcc
     1081 gccctcgttt ggcgtgtccg aggaagacga ggatggggag tcccagccgt ccgttccagg
     1141 ggataagacg ctcctcgatc tcagcgcgcc gtatcatatg ttgaagccga tgcggttgag
     1201 gattgatggc gtgccggtcg ggctcatgcg cttcgaggtg ttcgccatgt cggagagcgg
     1261 cgggggcggg caggtgatgg gcagcggctc gacgatcgca gagatcggga cgggcgtgca
     1321 gccggtgacg gtgaccctgc tcccgcacgg caatttggag ccgggcacgg tgctgccggg
     1381 tatcgataac gagccatcgg atgagaaccc ggcgccacgt tcgcggagcg cactggaggc
     1441 ggcccggcgg ttctggttgg ggttgcgccg gcggtagcga gcgcgcggga tggtcgttcg
     1501 gcgaggggct gcttccggca gcccctcgtt gtgagggaga gcgccggcgg ggccgcgccc
     1561 gccggcgcgt agttgtcacc cggtggggtg gcgtcgattc gcggaaaaaa gcggagtgga
     1621 tggagcgatg ccgatctacg agtatcgttg cgtgggatgc aaccgtaagt tcagccacct
     1681 acatggcatg gttgccaatg aggcgccgtt gcaatgcccg cggtgcggtg gcacggagct
     1741 gaagcggctc atctctcggg tatcgcgcgt gcggtcggaa gaggatgttt tcgacgctct
     1801 cgacccctcg aactttggcg acatggaaga tcctgccgat gtgcgtcgct gggcgcgcgc
     1861 gctcgggaaa gagatgggcg aggagttggg agaggatttc gaggagtcgc tggacgagat
     1921 gatcgaggcg gaggagcgcg gcgaactctc ggacgaggcg ggaggcgaag acgcgccggc
     1981 gtccccggcg cccgcggcaa gcagcgatga cctctgatac gccctcgggc cgcgccgccg
     2041 gcctgccgca tggggaagac gcgatgggcg agcgcccgac cagctccgac gaccgaggcg
     2101 cggccattcc agggcgcgtc ctggcggcca ccttcctcgt ggcgctcgcg ggcgtttgcc
     2161 tggaagtggc gctcgtgcgc gtcttcgcgc tccttttccg ctaccaccat ctcttcctcc
     2221 tcgtctcgg
//
LOCUS       DAOSCV010000782         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32048_length_2227_cov_2.336096, whole genome shotgun sequence.
ACCESSION   DAOSCV010000782 DAOSCV010000000
VERSION     DAOSCV010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32048_length_2227_cov_2.336096"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..269
                     /locus_tag="PLY56_16170"
     CDS             81..269
                     /locus_tag="PLY56_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23065.1"
                     /translation="MLAVAAALSLSLSGLVPSRHRANAEAPRTVYLREYAQFRASQEV
                     GDSTGLFLLASEMVREAQ"
     gene            282..1238
                     /locus_tag="PLY56_16175"
     CDS             282..1238
                     /locus_tag="PLY56_16175"
                     /inference="COORDINATES: protein motif:HMM:NF028497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HOJ23066.1"
                     /translation="MRWSSVLRSVAMVMMLLAWGAACEAQQPHSKSASHRWLRLMAAG
                     RQVPYEGVQTIEMHFAGRRTTATARVAHSRCATRTKILSPAHMRGMEILERGGRRLVR
                     RPGGTHWSASLGSIPPEPHRLLRNYRVSVQGTAETAGRDAIIGTISSRASGKQVRRIW
                     LDSEKGVVLRAEHYNWEGRLVYSTEFQEIDYAASLDEEEFETGKLPIRGPRAVPVEVP
                     DAELPKPSYVPAGFKLIGSPCSVRTKRGDAIHLRYGDGLNVISVFVRKQEPDDDRKKP
                     EPRPSQDEPFSAIVHRVKGGYRVTIIGDINPAELRRMADSID"
     gene            complement(1268..2191)
                     /gene="rbsK"
                     /locus_tag="PLY56_16180"
     CDS             complement(1268..2191)
                     /gene="rbsK"
                     /locus_tag="PLY56_16180"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOJ23067.1"
                     /translation="MPRILVVGSIHTDLVITTERLPARGETLLGGTFHTFPGGKGANQ
                     AVAASRLGGEVWMVGRVGADLYGSYQREHLAAAGVRTEFIATDPDAASGVALITVERS
                     GDNTIVVATGASGRCAPEDVDRAVSVLSQADVLLLQLEIPLETVEHAIHLAARAGTRV
                     ILDPAPARPLPDALLDQVDLLTPNETEAALLCGLDPAQPADPAELAARLREKTRASIL
                     LTLGAAGVLLATDQGITHVSAVPVTPVDTTAAGDTFTGALAVAWAEGAELPQAARWAS
                     HAASISVTRLGAQSSIPSRAEVEESVARAAR"
BASE COUNT          370 a          721 c          776 g          360 t
ORIGIN      
        1 ctgggtgacc aggcgcgtgc ccagcacctc ccactggccg ggcgatgtgg cggccacgcc
       61 ccgtactgct ggtgccggcg atgttggcgg ttgccgccgc gctgtctctt tctctttccg
      121 gcctcgttcc ctcgcgccat cgggcgaatg ccgaggctcc ccggacggtc tacctccggg
      181 agtacgcgca gtttcgcgcc agccaagagg ttggcgatag caccgggctc tttctcctgg
      241 ccagtgagat ggtccgagaa gcacagtagg gtgtgggagc gatgcgttgg tcatccgtgc
      301 tgaggtcggt cgcgatggtc atgatgctcc tggcgtgggg cgcggcgtgc gaggcccagc
      361 agccccattc caagagcgcc agccaccggt ggctgcgcct gatggccgcg ggtcggcagg
      421 tgccgtacga gggggttcag actatcgaga tgcactttgc cggccggcgg accaccgcca
      481 cggcgcgcgt ggcgcatagc cgttgcgcga cgcgcaccaa gatcctcagc ccggctcaca
      541 tgcgggggat ggagatcctc gagcgaggcg gccggcgtct ggtgcgccgg cccggcggca
      601 cccactggag cgcgagtttg gggagtattc cacccgagcc gcaccgtctc ttgcgcaact
      661 accgcgtgag tgttcaggga acggcagaga cggctggccg cgacgcgatc atcggcacga
      721 tctcgtcgcg tgcatcaggc aagcaggtgc gccgcatttg gctggattcc gagaagggcg
      781 tcgttctccg tgccgagcac tacaactggg aaggccgact cgtctactcc accgagtttc
      841 aagagattga ctacgcggca tcgctggacg aggaagagtt cgagaccggg aagctgccga
      901 tccgtggccc ccgggccgtg ccagtcgaag tgcctgacgc ggagttgccc aagcccagct
      961 acgtgccggc ggggttcaag ctgataggga gtccctgcag cgtgcgcacc aagcgcggcg
     1021 acgcgattca ccttcgctat ggcgacggcc tgaacgttat ctccgttttt gttcgcaagc
     1081 aagagccgga tgatgatcgg aagaagccgg agcctcgtcc gtcgcaggac gaaccattct
     1141 ccgcgattgt ccaccgcgtg aaaggcggct accgcgtgac gatcatcggt gatatcaacc
     1201 cggcagagct gcgccgcatg gccgattcga ttgattgacg cccttcactg aatggggcgc
     1261 gagtggttca gcgggcagcc ctggcgacgc tctcctccac ctccgcacgc gaggggatcg
     1321 aggattgtgc gcccagccgg gtgacggaaa tggaagcggc atgcgaggcc cagcgggcgg
     1381 cctggggcag ctccgctcct tccgcccagg ccacggcaag cgcgccggtg aaagtgtcgc
     1441 ccgcggcggt ggtatcgacc ggcgtgacag ggaccgccga cacatgcgtg atcccctggt
     1501 ccgtggccag caggacgccg gcggcgccca acgtgaggag gatgctggcg cgcgtcttct
     1561 ctcgaagccg ggccgcaagc tcggcggggt cggccggctg tgccgggtcg aggccacaca
     1621 gcagcgccgc ttccgtctca ttgggcgtga ggaggtcaac ctggtcgagc agggcatcgg
     1681 ggagtggccg ggccggggcc ggatcgagga tcacgcgcgt ccccgcccgt gccgccaggt
     1741 ggatggcatg ctccacggtc tcaagcggga tctccaactg cagcaggagc acatcggcct
     1801 gcgaaaggac gctcacggca cgatcgacat cctcgggagc acaccggccg ctggccccgg
     1861 tggctacgac gatggtgtta tcaccgctcc tctcgacggt gatcagcgcc actcccgagg
     1921 ccgcatccgg gtcggtggcg atgaactccg tgcgcacgcc cgccgcggcg agatgttcgc
     1981 gctgatagct tccgtagaga tccgcgccaa cgcgcccgac catccagacc tcgccgccga
     2041 ggcgagatgc ggcaaccgcc tgattcgcgc ctttgccgcc ggggaaagta tggaaagtgc
     2101 ccccgagaag cgtctcgccg cgcgccggaa ggcgctcggt ggtgattacc agatcggtgt
     2161 gaatgctgcc gacgaccagg atgcggggca ttcgagcctc ctcatacgaa cggaagatag
     2221 ccgaaaa
//
LOCUS       DAOSCV010000783         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32096_length_2224_cov_2.978331, whole genome shotgun sequence.
ACCESSION   DAOSCV010000783 DAOSCV010000000
VERSION     DAOSCV010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32096_length_2224_cov_2.978331"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLY56_16185"
     CDS             complement(<1..518)
                     /locus_tag="PLY56_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007303651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23068.1"
                     /translation="MLKQSRGAALWISALALMISLVGVTTARQAGAQERGPLTREQAR
                     ALEQLEEAFTAIADAVEPVVVQIKVDAARPRFRSNEENEGMPDRPDDFFRQFPFGPNL
                     RPVPPRGAGGTGSGVIVKIDGHDAYILTNAHVVNGASNDAITVVLQDGTTISGKSKVT
                     VRGIDTKTDLAV"
     gene            complement(746..>2224)
                     /locus_tag="PLY56_16190"
     CDS             complement(746..>2224)
                     /locus_tag="PLY56_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ23069.1"
                     /translation="GWMLILVMSAYNATGDPFYLNAAKRMIERVREKSHPDEGFYAHP
                     LPRGHCECDPPHRGEAGFMMGVLMTGMKMYYEVTGDERVADDIVKAARFLVKTMWEPE
                     KLGFRYTSCPKTNVTYSSATIELEGIAFAARRANDPALAEICQQALAAALPTLSGAGK
                     GMGFYLRSAPQMLDAVAALPGPSFDDYRRRMEALVRSPARRPYPGPLGNPDYEEGHDG
                     WGARTGFSLAIAPEAAHSGRAGARVTGKGRGQNEYLVTIYDTGGTNPMEITGLEPGKT
                     YRVSAWVKVDRITPGAPAPSIRCSIRDRERTRESFVSNAYDLNRLGTWQRLERVFQAP
                     DHTFSAYVAVNTNTREEVEIDLSVDDWSLAPAGPDAAATPARLVADAATATLGEGTTR
                     ERIAPPLGFAAVSGGEARWTLQVPREGEYYLWARVAGAGAELRIGNTTVSLKPAGCEW
                     NWVRWERPLRLPAGECVITARLVGKDARLSRIVVTDEALAAR"
BASE COUNT          347 a          781 c          708 g          388 t
ORIGIN      
        1 aacaccgcca ggtcggtctt cgtatcaatc ccgcgcaccg tgaccttcga cttgccggag
       61 atggtcgtgc catcctgcaa gaccacggtg atcgcatcgt tggatgcgcc gttcacgacg
      121 tgcgcattgg tgaggatgta ggcgtcgtgg ccatcgatct tcacgataac gcccgacccg
      181 gttccacccg cgccacgcgg gggcaccgga cgcaggttcg ggccgaacgg aaactggcgg
      241 aagaaatcgt ccgggcgatc cggcatgccc tcgttctcct cgttactgcg gaagcgcggg
      301 cgtgccgcat ccaccttgat ctgcaccacc accggctcga cggcatccgc gatggcggtg
      361 aacgcttcct ccagttgctc aagggcccgc gcctgctcgc gcgtcagcgg gccgcgctcc
      421 tgcgcgcccg cttgccgggc ggtcgtcaca cccaccaatg agatcatgag cgccaacgcg
      481 ctgatccaca gcgccgctcc cctgctctgt ttcagcatct cgctcactgt tgccaacctc
      541 ccgctttcaa ctttcaacac ctgggtatgc gcctcgggcc tgctcccacg agaaccctct
      601 ctgccggtta ctctaataga cccatgctgc cgtcccgcgt tcccaaaggg acttttctgc
      661 gagggagcgc atcgcagggc cggtgggaga cggcatccgg ccatgccacc ggcatccttt
      721 cagacgcgac ggcactcgca agggttcacc gcgcggccag cgcctcgtcc gtgaccacga
      781 tccggctcaa acgcgcatcc tttcccacca gacgcgcggt gatgacgcat tcgccggcag
      841 gcagccggag cgggcgttcc cagcggaccc agttccactc gcatcccgcg ggcttgagtg
      901 agacggtcgt attccctatc cggagctccg ccccggcccc tgccacgcgc gcccacaggt
      961 agtactctcc ctcgcggggt acctgaagtg tccagcgggc ctcacccccg gaaaccgcgg
     1021 cgaagccaag gggtggcgcg atgcgttctc gcgttgttcc ttctccgagc gtcgccgtgg
     1081 cggcatcggc caccaggcgc gccggcgtcg ccgcggcgtc cggaccggcc ggggccaggc
     1141 tccagtcatc cacgctgaga tcgatctcga cctcctcgcg cgtgttcgta ttcaccgcga
     1201 cgtaggcgga gaaggtgtgg tcgggcgctt ggaacacccg ctccaggcgc tgccaggtgc
     1261 cgaggcgatt cagatcatag gcgttggaga cgaagctctc gcgcgtgcgc tcgcggtcgc
     1321 ggatggagca gcggatacta ggcgcgggcg cgcccggggt gatccggtcc accttcaccc
     1381 aggcggagac gcgatacgtc tttcccggct ccaggccggt gatctccatc gggttggtgc
     1441 cgccggtgtc gtagatcgtc accagatatt cattttgccc cctgcccttg cccgtgacgc
     1501 gggcgccggc gcgcccggag tgcgcggctt ccggcgcgat cgccagagag aagccggttc
     1561 gcgcccccca gccgtcgtgc ccttcctcgt aatccgggtt gccgagaggc cctgggtacg
     1621 gccggcgcgc tggcgagcgc acgagcgcct ccatgcgccg gcgatagtca tcgaaagagg
     1681 ggcctggcaa ggcggcgacg gcgtcgagca tctgcggtgc ggagcgcagg tagaagccca
     1741 tccccttccc cgccccggag agggtcggca gagccgctgc gagggcctgc tggcagatct
     1801 ccgccagggc cgggtcgttg gcgcggcgcg cggcaaacgc gatcccctcc agctcgatcg
     1861 tggcggagga gtaggtgacg tttgtcttcg gacaggacgt gtagcggaat ccgagcttct
     1921 ccggctccca catcgtcttc accaggaagc gcgccgcctt cacaatatca tcggctaccc
     1981 gctcgtctcc ggtcacctcg tagtacatct tcatgccggt catcagcacg cccatcatga
     2041 agccggcctc gccacgatgc ggagggtcgc actcgcagtg gccacgggga agcgggtggg
     2101 catagaaccc ttcatcgggg tgcgatttct cacgcacgcg ttcgatcatc cgcttcgcgg
     2161 cattgaggta gaacgggtca ccggtggcgt tgtaggcgct catgacgaga atgagcatcc
     2221 accc
//
LOCUS       DAOSCV010000784         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32194_length_2220_cov_3.754734, whole genome shotgun sequence.
ACCESSION   DAOSCV010000784 DAOSCV010000000
VERSION     DAOSCV010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32194_length_2220_cov_3.754734"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLY56_16195"
     CDS             complement(<1..265)
                     /locus_tag="PLY56_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23070.1"
                     /translation="MRLHLLPLLAIAFAAGIALSGRWMLPPVPPAVAAVSAVLVVCAA
                     LRWAPRLTLLSLLALGLSGGAFWEALDRALHPNLLQPWVGKRVE"
     gene            complement(474..1772)
                     /gene="hemA"
                     /locus_tag="PLY56_16200"
     CDS             complement(474..1772)
                     /gene="hemA"
                     /locus_tag="PLY56_16200"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOJ23071.1"
                     /translation="MRVILVGANHQTAPLAVRERLAFTKASLAPALLQLREGGEIEEA
                     AILATCNRTEVYAAGRQASVAPILRLFRARGVSERELTPCLYTRHDEDAIRHLHRVAA
                     GLESMILGEAQILGQVKAAWCAALDAGAVGPVLDRLFRSAVTAGKRVRAETGLARGAV
                     SVGHAAVELARRTFGQLEGLHAMVLGAGEMSKVAARLLVDAGVASLTVANRTLERAQD
                     LAAELGARAIRVAEFGRHVGKMDIVICSTAAPHPVVRAETIRPVAAARRGRPLLLVDI
                     AVPRDVEPAVGNLENVHLFTIDDLQSIVQENLAGRAAEVEAAEAIVAEEAAKLVEWLR
                     CFQVGPVLAALQRKGESVVQDELAKIEGRLRHLSDRDRELIGVLARGVARRLLREPIL
                     AVKRLAATEEPRPPLETVQELFGLSDVGAPTPDPAGEHSD"
     gene            complement(1717..>2220)
                     /locus_tag="PLY56_16205"
     CDS             complement(1717..>2220)
                     /locus_tag="PLY56_16205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOJ23072.1"
                     /translation="ELRARAYAPEDLDGVFLAIAATDSPAVNTAVSEDARARKILVNS
                     VDRPDECDFITPASVRRGDLLISIFTGGKSPGLSRRIRERIEEVIGPEYAEFVALLGA
                     LRPGVMAAYATEQERSRVWRRLLDSDVLALLRAGRAAEAWERAREIAYACDPGGSEPP
                     DRPPGGA"
BASE COUNT          352 a          811 c          753 g          304 t
ORIGIN      
        1 cctccacgcg cttcccgacc cacggctgaa gcagattcgg atggagcgca cggtcgaggg
       61 cttcccagaa ggctcccccc gaaagcccca gggcaaggag cgagaggagc gtcagtcgcg
      121 gcgcccaccg gagagcggcg cagacgacga gcaccgcgct cacggccgcc acggcaggcg
      181 gcactggcgg cagcatccaa cgcccggaaa gggcgattcc ggccgcgaaa gcgatcgcca
      241 gcaggggaag caagtgcagg cgcatgggcg gccacccatc acgacgctaa gcggcacgcc
      301 ggggttctac gtgccacaca aatcggcgcg tcctgccgcc ctcctgttcg gcaaagtgga
      361 gcgcgctcct tcgagtcgcg cgagagaaga gagaatctgc cctgccagcg ccttcgcgcc
      421 gcgggcgagc ggttgcccag gtgttcagcc gcagatggga ggacgccggt cggtcagtcg
      481 gagtgttctc ctgccgggtc cggcgttggc gcgccgacat cggaaagccc gaagagctcc
      541 tgaaccgtct ccaggggcgg gcgtggctct tccgtggcgg cgaggcgctt cacagccagg
      601 atcggctcgc ggaggaggcg ccgggccacg ccgcgtgcca gcacgccgat caactcgcgg
      661 tcacgatccg agaggtggcg caaccggcct tcgatcttcg ccagctcgtc ctggaccacc
      721 gactcaccct tgcgctggag cgcggccagc accgggccga cctgaaaaca tcggagccat
      781 tcgaccagct tcgcggcctc ttccgccacg atcgcttctg cggcctccac ctcggcagcg
      841 cgcccggcca ggttctcctg aacgatcgac tgcaggtcgt cgatggtaaa gaggtggacg
      901 ttttcgagat tgcccacggc gggttctacg tcgcggggca ccgcgatgtc aacgagcagg
      961 agggggcggc cccgccgggc cgcggcgacg ggccggatgg tctccgcgcg caccaccgga
     1021 tggggcgccg ccgtcgagca gatgacgata tccatcttcc ccacgtgccg gccaaactcg
     1081 gcgacgcgga tcgccctggc gcccagctcg gccgccaggt cttgggcacg ctcgagcgtg
     1141 cggttggcga cggtgagcga ggcgacgccc gcgtccacga ggagacgcgc ggccaccttg
     1201 ctcatctcgc ccgcgccgag caccatcgcg tggaggcctt ccagctggcc gaaggtccgc
     1261 cgggccagct ccacggcggc atgacccacg gagacggcac cgcgtgccag tcccgtctcc
     1321 gcgcgcacgc gcttgcctgc tgtgacggcg ctgcgaaaga ggcggtcgag caccgggccc
     1381 acggcgcccg cgtccagcgc ggcgcaccat gcagccttca cctggcccag gatctgcgcc
     1441 tcgccgagga tcatcgactc gagcccggcg gccacgcgat ggaggtgtcg gattgcgtct
     1501 tcgtcatggc gcgtgtagag gcagggggtc agctctctct cggaaacgcc gcgcgcgcgg
     1561 aagaggcgta ggatcggcgc cacgctggcc tgccgccccg ccgcatagac ctcggtgcgg
     1621 ttgcaggtgg ccaggatcgc cgcctcttct atctccccgc cctcgcgcag ctggaggagg
     1681 gccggagcca gactcgcctt cgtgaacgcc aggcgctcac gcaccgccag gggggcggtc
     1741 tggtggttcg ctcccaccag gatcacacgc atacgctatc tcccgcgctc gttcccacgc
     1801 ctcggcagcg cgtccagcgc gcagcaacgc cagcacatcc gaatcgagga ggcgccgcca
     1861 cacgcgcgac cgctcctgct ccgtggcgta agccgccatc acccccgggc gcaacgcacc
     1921 cagcaacgcc acgaactcgg catactccgg accgatcacc tcctcgatcc gctcgcggat
     1981 ccgccgcgaa agccccgggc tctttccccc ggtgaagatg ctgatcagca ggtcgccccg
     2041 gcgcacgctc gccggggtga tgaagtcgca ctcgtccggc cggtccacgc tgttcacgag
     2101 gatcttccgg gcgcgcgcgt cctcggagac ggcggtattc actgcgggcg aatcggtggc
     2161 ggcgattgcc agaaagacgc cgtccaggtc ttccggcgcg taggcccgcg cgcgtagctc
//
LOCUS       DAOSCV010000785         2218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32239_length_2218_cov_3.022654, whole genome shotgun sequence.
ACCESSION   DAOSCV010000785 DAOSCV010000000
VERSION     DAOSCV010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32239_length_2218_cov_3.022654"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="PLY56_16210"
     CDS             <1..1406
                     /locus_tag="PLY56_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOJ23073.1"
                     /translation="DVIAVDDGRREYQRVERIRNLRPDMILLSGGTDGGTVTHLVELA
                     ELLVAADPRPRLGIGLQIPVIFAGNKAAREAVARTVDGKCDLRIVDNLRPTMERENLG
                     PAREAIHELFLQHVMQQAPGYSKLSSWTSTGIMSTPNAFGQMVQTIADSLGLSVLAVD
                     IGGATTDVFSVFDGTFNRTVSANLGMSYSICNVLLEAGTANIARWVPFPLEDADLRNR
                     LRNKMIRPTTIPHTLADLRIEQAVAREALRLAFEHHKALARGLKGVQQVRTIGEALSQ
                     SGTGETLVDLMELGLIVGSGGVLSHAPRRAESALMMLDAYQPEGVTLLAVDSIFMMPQ
                     LGVLSTVLPEAAMQVFERDCLIKLGHSIAPVGTMRPGETAVTIRINGSETRVPFGSIE
                     VLPLGVGEQAEVEILPGRGLDVGAGKGRPRRATVEGGVVGLIIDARGRPLQLPTDPDA
                     RIAKLTEWHQALGLPVA"
     gene            complement(1979..>2218)
                     /locus_tag="PLY56_16215"
     CDS             complement(1979..>2218)
                     /locus_tag="PLY56_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23074.1"
                     /translation="IPRGHLPLEPGPVEAGAVIFHGDMDPLGMRLHGDAAVRRTTATP
                     VGESVGDGFRKGCEAFSDGHLRQAKRGAYEGLCAV"
BASE COUNT          347 a          747 c          734 g          390 t
ORIGIN      
        1 tggacgtgat cgccgttgac gatggccgca gggagtatca gcgggtggag cggatccgca
       61 acctccgccc cgacatgatc ctcctctcgg gcggcacgga tggcggcacc gtgacgcatc
      121 tcgtggagct ggccgagctt ctcgtcgccg ccgacccgcg gccacgcctg gggatcgggc
      181 tgcagattcc ggtgatcttt gccgggaaca aggctgcgcg cgaggcagtg gcgcgaacgg
      241 tcgatgggaa gtgcgatctc cgcatcgtcg ataacctccg gccaacgatg gagcgcgaga
      301 acctggggcc ggcgcgagag gcgatccacg aactcttcct gcagcacgtg atgcagcagg
      361 cgcccggcta ttccaagctc tcctcttgga cttccaccgg gatcatgtcc acgcctaacg
      421 ccttcgggca gatggtccag accatcgccg acagccttgg cctgtcggtg ctcgcggttg
      481 atattggcgg ggcgactacg gatgtcttca gcgtcttcga tggaaccttc aaccgcaccg
      541 tttcggccaa cctggggatg agctactcga tctgcaacgt gctcctggag gcgggcaccg
      601 ccaacatcgc gcgttgggtc cccttcccgc tggaggatgc cgacctgcgc aaccgcctgc
      661 gcaacaagat gatccggccc acgacgatcc cgcacacgct cgccgacctg cgcatcgaac
      721 aggccgtcgc gcgggaggcg ctccgtcttg ctttcgagca tcacaaggcg ctcgcgcgcg
      781 ggctgaaggg cgtccagcag gtgcgcacca tcggggaagc gctgtcgcaa tctggcaccg
      841 gcgaaacgct ggtcgacctc atggagctgg gcctgatcgt cggcagtggc ggcgtccttt
      901 cgcatgctcc ccgccgcgcc gagtcggcgc ttatgatgct ggatgcctat caaccggagg
      961 gcgtgacgct tctggccgtg gactccatct tcatgatgcc gcaactgggc gtcctctcca
     1021 ccgtccttcc ggaagccgca atgcaggtgt tcgagcgcga ctgcctgatc aagctcgggc
     1081 attccatcgc gcccgtaggg acgatgcgcc ccggcgaaac cgccgtcacc atccggataa
     1141 acggctcgga gacgcgcgtg ccgtttggga gcatcgaggt cctgccgctg ggcgttggag
     1201 agcaggcgga ggtggagatc cttccggggc gtggcctcga cgtcggcgcc ggcaagggcc
     1261 gcccgcgccg ggccacggtc gaaggcgggg tggtcggact gatcatcgat gcgcgcgggc
     1321 gtccattgca gctgccgacg gatccggatg cgcgaatcgc caagctcacc gagtggcacc
     1381 aggcgctcgg ccttccggtc gcctagagaa tagcgtctcc ctgtgcgctc ggcgggaaga
     1441 ctctcgcgcg tggagctgaa ggcccgcgcc gctacccgac aaggcccttc gggctccccc
     1501 tcctggcgtc tgcctatctg ggttgcccag ggatatagcc cggcccggct tcgcccgcaa
     1561 gcagcgcggc ggtttcgccc cgcgcgacgc ctcaccttct cagcccgcac cttcctggat
     1621 tgcccccgaa tgcagcccgg cccaacccgc cagggttttg ttcgcaagca gcgcgggcct
     1681 ttagccctac gcgatccacg gggtggccgg cttgcgcccg cgtttccctc tactctccag
     1741 ccggcgccag cgcagtgtcg cggggccgtc agcacccctg ggcatggagc tgcaggcgcg
     1801 actcaagctt gatgcttcct tgcccgaatg atataatagc cctgggagca ctcggcagac
     1861 tgccggcgct gtcgaatgca ggtgggtgcc gtgggccgtg ccaggggttg gtgcctcagc
     1921 agagaggtgg agaacaaccg tggtggttgg caaggtgtat ctggagtcta ttgaggatct
     1981 acaccgcgca aaggccctcg taagccccgc gcttggcctg tcggagatgg ccgtccgaga
     2041 atgcctcaca gccctttcgg aagccgtcgc caacgctctc acccacgggc gtcgcggttg
     2101 tcctccgtac tgccgcgtca ccgtgcaggc gcattcccag tgggtccata tcaccgtgga
     2161 agatcacggc gccggcttcg actgggccgg gctcgagcgg gagatgccct cttgggat
//
LOCUS       DAOSCV010000786         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32299_length_2215_cov_3.800463, whole genome shotgun sequence.
ACCESSION   DAOSCV010000786 DAOSCV010000000
VERSION     DAOSCV010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32299_length_2215_cov_3.800463"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLY56_16220"
     CDS             complement(<1..445)
                     /locus_tag="PLY56_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOJ23075.1"
                     /translation="MATKSLLLPVLLPGGEECIRCAEELRGRMLVVPGVRAVEVDRAA
                     RQLVVTYEGSAITPERIEEAAELAGAEVAKHRCHETLILRGLDCADCAQKVERAVLAI
                     PGVEHAELSFAASRLAVEYDPRRVSYADITRVVTDLGYRVEEARAP"
     gene            complement(492..836)
                     /locus_tag="PLY56_16225"
                     /pseudo
     CDS             complement(492..836)
                     /locus_tag="PLY56_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(1577..>2215)
                     /locus_tag="PLY56_16230"
     CDS             complement(1577..>2215)
                     /locus_tag="PLY56_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ23076.1"
                     /translation="RHGFTLIELLVVIAIIAILAAILFPVFARARENARKSNCASNLK
                     QLGLAILAYAQDYDEMFPKYTLKNVPTDKNDWHEVIDPYVKNTLLKRCPSIKGSTLGY
                     GANWRHIICYPKEDLWAGLDVTLAQFQRPSEALVLSDSHSGTTGEGTSGMAAIYCPHC
                     YSTPPYDYVNYAVSSRHSEGANCLMLDGHVKWYKTTTILETDASSIWAHNNP"
BASE COUNT          385 a          744 c          682 g          404 t
ORIGIN      
        1 ccggtgcgcg tgcctcttcc acacggtagc ccagatccgt cacgacgcgc gtgatgtcag
       61 cataggagac gcggcgcgga tcgtactcca ccgccagccg cgaggccgcg aaggagagct
      121 ccgcgtgttc cacgcccgga atggcgagca ccgcgcgctc caccttctgc gcgcaatcgg
      181 cgcaatccag accgcgaagg attagcgtct cgtggcagcg atgcttggcc acctccgcgc
      241 cggccagctc cgcggcctcc tcaatccgct ccggggtgat tgcagagccc tcgtaggtga
      301 ccacgagttg gcgggcagcc cggtctacct ccaccgcgcg cacaccgggc accaccagca
      361 tgcgcccccg tagctcctcg gcacagcgga tgcactcctc accgccgggt agcagtaccg
      421 gcagcagcag gctcttcgtc gccactgctc tcctcgcctt tcccctccgc ccccgttccc
      481 gcgcggtgct ccatatgctc gagcccctgc tggaagaggg tgcggatatg atcgtcatcc
      541 agagtgtagt atacccaccg accgtctcgg cgcgagcgca ccagccgcag cgagcgcaac
      601 agccggagct ggtgagagac cgcggacgca gtcaccccca agagcgccgc caattcaccg
      661 acgcacagtt ccgcgcgcga gagtgcgaag agcagcttca cccgcgtccg atcccccatc
      721 gcctggaagg tctccgcgag gtcagcaaag accgcgtccg gcaccatctg gcgctgcacc
      781 gcctccacgc gctccctact cgcatgcgag agttcaccgc tctcgtccgc gtccatgcat
      841 cccctccgca cacctgaata gttgctcaaa tctgcattca tcataccacc gccgtgtgaa
      901 gccgtcaagg aatttggcgg gcgcattgag attccaagag aaggcaggcc ggggcggttg
      961 cctgcgttct tccgccctcg cctttatgcc gcgcgccggg ttgcatgtca cacgcgcgcg
     1021 gcgagcgaag ctccgcgctg cttccaggca aagccccttc ggtccccctc ccaggctacc
     1081 gccaccaaac cgcgggtgac gtggagggtc caacgtttgc tattcgccct gcttccgggc
     1141 aggcacccgg gggacgcaat ccatccggga tcggcgcatg gcccgggcga gacgctctct
     1201 atggaccacg aagtggtccc acaatagagc ccatgaccct gtctcgcttc gaggtcgtgg
     1261 ttcttgtttt cggcacatga cccagggtgc ggacaccctg ggctttgttg cgcggccctt
     1321 tcggggccgg ggctgggccg cccgcaagct atgggagaag aggctggaga ggtccgggca
     1381 gtgtctgccc aaaccggatt ccttatctca ggcgggttgg atttgagggg agtgacgccc
     1441 cgagcgagag gaacccgaat cgatgctcat ctgcggattt catccgaccg tgcggcggtg
     1501 gcgagccgga cagcggaaag cagtcgcgag gaggcaactg gcgatcccgc gactgcttcc
     1561 cgcctctgag ggcttgctag ggattgttgt gcgcccagat ggaggacgca tccgtctcca
     1621 gaatggtagt cgtcttgtac cacttcacat ggccgtccag catcaggcag ttcgcgccct
     1681 cgctgtgccg agaggagacc gcgtagttca catagtcgta cggcggcgtg gagtagcaat
     1741 gcgggcagta gattgccgcc atcccgctcg tgccctcgcc cgtcgtgccc gagtggctgt
     1801 cggagagcac cagcgcctcg gacgggcgct ggaactgcgc gagggtcaca tccaggcctg
     1861 cccacaaatc ctccttcggg tagcagatga tgtgccgcca gttcgcccca tagccaaggg
     1921 tggatccctt gatgctcggg caccttttga gcagcgtgtt cttgacgtag gggtcgatca
     1981 cttcgtgcca gtcatttttg tcagtgggca cgttcttcag cgtgtacttg gggaacatct
     2041 cgtcgtagtc ctgcgcataa gcgaggatcg ctaggcccag ttgcttcagg ttcgaggcgc
     2101 aattggactt tcgcgcgttc tccctggccc gggcaaagac cggaaagagg attgccgcga
     2161 ggatggcgat aatcgctatc acaaccagga gttcgatcag agtgaaaccg tgcct
//
LOCUS       DAOSCV010000787         2215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32300_length_2215_cov_3.761574, whole genome shotgun sequence.
ACCESSION   DAOSCV010000787 DAOSCV010000000
VERSION     DAOSCV010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32300_length_2215_cov_3.761574"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLY56_16235"
     CDS             complement(<1..703)
                     /locus_tag="PLY56_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ23077.1"
                     /translation="MRRVILLLLIAVAVPILAGNGARARFGRLVGTLSEGPGCARLLL
                     ASALASEAGWRAEERRYQELRQKLVRQQPDYRVWQRSPDELWVYDNRPELGIGMHGMT
                     DKQLDLVRALGIRLVRITLYWQEMEKTETPGVYDAKYLRHWDELVARAKKHGVELLVV
                     VHGNPPGVGWATREESYRRFARFMGDMASRYPGVCFWELWNEMDSGFTDLFGAGVNPS
                     VPMRERGKHYARMLKL"
     gene            1066..1428
                     /locus_tag="PLY56_16240"
     CDS             1066..1428
                     /locus_tag="PLY56_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ23078.1"
                     /translation="MKRYRLNQLPDVTEGHFLKGIIPGEYLCKGGLSFKKPNARTHTN
                     DGPGGKDWHVHEDDCEVFILLQGKAIMEVNGERIPMVTGDVFVIEPGEDHHLISDAED
                     PCVNLWLHAGPNRHPDQL"
     gene            complement(1698..>2215)
                     /locus_tag="PLY56_16245"
     CDS             complement(1698..>2215)
                     /locus_tag="PLY56_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23079.1"
                     /translation="LAGLPAQIGIRETRRILGEHLLQEQELLEGVRFPDAIAQGTYPV
                     DIHNPRGRGIVFKHLDGRWREVLPDGSRREGTWTPDGQVQKTPCYQVPYGVLVPRKVE
                     NLLVAGRCISATHEAHGAIRVMVNCMQFGQAAGAAAALCVREGYAPRELPAPLLRRLL
                     LEQGCCFLGEG"
BASE COUNT          398 a          783 c          687 g          347 t
ORIGIN      
        1 cgagcttgag cattcgcgcg tagtgcttgc cacgctcgcg catcggcacc gaaggattga
       61 cgcccgcacc aaagaggtcg gtgaacccgg aatccatctc gttccacagc tcccagaagc
      121 agacgcccgg atagcggctg gccatgtcgc ccatgaagcg ggcgaagcgc cggtagctct
      181 cctcgcgcgt tgcccacccc acgcccggtg gattgccatg caccaccacg agcagctcca
      241 ctccgtgctt tttggcccgg gcaaccagct cgtcccagtg gcggagatac ttcgcgtcat
      301 acacgccggg cgtctccgtc ttctccatct cctgccagta gagcgtgatc cgaaccaggc
      361 ggatccccag cgcccggacc agatcgagct gcttgtcggt catcccgtgc atgccaatgc
      421 ccagctccgg acggttgtca tatacccaca gctcgtcggg cgaccgctgc catactcggt
      481 agtccggctg ctggcgcacc agcttctgcc gcagctcctg ataccgccgc tcctccgcgc
      541 gccagccagc ctcactcgca agcgcgcttg ccagcaggag gcgcgcgcat cccggaccct
      601 ccgagagcgt accgacgagg cgcccgaaac gcgccctcgc gccattcccg gccagaatgg
      661 gaaccgcgac cgctatcagc aggagaagta tcacgcgtcg catcggcgaa acctccgaat
      721 aaggacgagg cagggcccgc gccagccgac cagggcagcc tgccctatgc ctagcggcat
      781 agaatccgtg cgattgttcg gctacaggct catcatatcg cgcgcgcccg cagggtgtca
      841 agcgatgcgc cgcatgcgtc gcgcggaagt ggatgtcgaa ggcgcgacgg tgaccggtgc
      901 agaaagaatg aacacaggtg gcaggcgaag gtccagccgg gcgaacagta accgctcgcc
      961 cctgtggccg tgccgggata ggtgtccccc cacctggtcc ggatgctgaa caagcacccg
     1021 gaagcctgcg cctcaagaga agcagcgtgt gagaggagca ccttcgtgaa acgttaccga
     1081 ttgaaccagt tgcctgacgt aaccgagggc cacttcctga aggggattat ccctggcgaa
     1141 tacctctgca agggcgggct ctccttcaag aagcccaacg cgcgcaccca caccaacgac
     1201 ggcccgggcg gcaaggattg gcacgtccac gaggacgact gcgaagtctt catcctcttg
     1261 cagggcaaag cgatcatgga agtcaatggc gagcgcatcc cgatggtcac cggcgatgtc
     1321 ttcgtgatcg agccgggcga ggaccaccac ctcatctcgg acgccgagga tccctgcgtc
     1381 aacctctggc tccatgcggg ccccaaccgg cacccggatc agctctgagt ggtcccccgg
     1441 tctcggactc cccatcgcat agggtcgggg gcactcgcgc cagggggccc gcgccctatg
     1501 ctgagaggcc ggcgccgcag cggcgcgcgg ggatcaatcc ccgcgctgct tgtgagcgaa
     1561 gccctgccgg gctggcggtc tttacgttct cgggcgatcc tcgcaggcgt gggccgggag
     1621 ggagagcccg aagggctttg ccaggaagca gcgcggagct tcagctccgc ccctgcgtgt
     1681 gcaacaaccc cgcgacccta tccctctccc aggaagcagc acccctgctc cagcaggagc
     1741 cggcgcagca gtggggcggg cagctctcgg ggcgcatatc cctcgcggac gcaaagggca
     1801 gcggcagccc ctgccgcctg tccaaactgc atgcagttca ccataacgcg gatcgcgccg
     1861 tgggcctcgt gcgtcgctga gatgcaccgg ccggcgacca gcagattctc caccttccgg
     1921 ggcaccagaa cgccgtaggg cacctggtag caaggggtct tctgcacctg gccatcgggt
     1981 gtccaggtgc cttcgcggcg cgatccgtcc ggaagcacct ctcgccacct gccgtcgaga
     2041 tgcttgaaga cgattccccg ccctcgtggg ttgtggatat ccaccgggta ggtcccttgc
     2101 gcgatggcgt ccgggaagcg cacgccctcc agcagctcct gctcctgcaa caggtgctcg
     2161 cccaagatgc gccgtgtctc ccggatcccg atctgcgccg gcagccccgc caggt
//
LOCUS       DAOSCV010000788         2211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32385_length_2211_cov_3.200371, whole genome shotgun sequence.
ACCESSION   DAOSCV010000788 DAOSCV010000000
VERSION     DAOSCV010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32385_length_2211_cov_3.200371"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLY56_16250"
     CDS             <1..1221
                     /locus_tag="PLY56_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOJ23080.1"
                     /translation="TLQHDLAAEAQAFAEQCPIGGQTIHTGATSADITDNADALIIRQ
                     ALELVRLRLANLLRAYEQRISEVYQERLVALGFTHIQPAQPVPLAYRLANHAQDLLRV
                     LWQLEGFQIEGKGTKGAVGTGADYTTMLDGTGVSFLEFHERVMQKLDLPALTVTGQTA
                     TRLVDVEYMNILYGLAATLHRAFTDQRILQSMGETQEPFAKGQKGSSVMAYKKNPKDS
                     EQICALSAAVMSFHSMAGYAAANNLLERTLNESAIRRVFIPEAFLYIDHCLIKAHKVI
                     SGLVINREKIARDLRTHGDFAGTGRLLVRLQQMGVPREEAYRMIQSCSLQAQEAVVRG
                     EENPLRGLLLEAIHALPYPEARTLTAEEIATLLDASAYVGGSFELTEEFLSVLRGALS
                     AYPEVQQDASESVF"
     gene            complement(1535..>2211)
                     /locus_tag="PLY56_16255"
     CDS             complement(1535..>2211)
                     /locus_tag="PLY56_16255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ23081.1"
                     /translation="FPYQGVLTGLLGVSARTRPGEGTATLWVRYQDGSHEKFPLSLTV
                     TSANFPTQRIRMAKSKTGLMSPEILKKEREILNEALASTAPEPLWEGPFANPLEGRIT
                     SPFGRERYVNGRFWGFHSGRDVASPAGRPVKAANTGRVTLARKLWMRGNTVAIDHGMG
                     VFTLYEHLSRFSVTPGQQIKKGEVVGLVGSTGFSTGPHLHWELRIHGVPTNPAGPLRE
                     GISLTP"
BASE COUNT          377 a          729 c          729 g          376 t
ORIGIN      
        1 accctgcagc acgacctcgc tgccgaggcg caggcgtttg ccgagcagtg ccccatcggc
       61 gggcagacga tccataccgg ggctacctcg gcggacatca ccgataacgc cgatgcgctg
      121 atcatccggc aggcgctgga gctggtgcgc ctccgcctgg cgaatcttct tcgcgcttac
      181 gagcagcgga tcagcgaggt gtaccaggag cgcctggttg ccctcggctt cactcatatt
      241 cagccggcgc agcccgtacc ccttgcgtat cgcctcgcca accacgccca ggacctgctg
      301 cgcgtgctgt ggcagctcga aggtttccag atcgagggca agggtaccaa gggcgccgtg
      361 ggtaccgggg cggactacac cacgatgctg gatggcactg gcgtcagctt cctcgagttc
      421 cacgagcgcg tgatgcagaa gctcgatcta ccggcgctga cggtgaccgg gcagaccgca
      481 acgcggctgg tcgatgtgga gtacatgaac atcctctacg gactggcggc gacgcttcac
      541 cgcgccttca ccgaccagcg catcctgcaa tcgatgggtg agacgcaaga gcctttcgcc
      601 aaggggcaga agggctccag cgtgatggcc tacaagaaaa acccgaaaga ttccgagcag
      661 atctgcgcgc tgtcggcggc ggtgatgagc ttccactcca tggccggcta cgcggcggca
      721 aacaacctcc tggagcgcac gctgaatgag tcggccatcc gccgtgtctt tatcccggag
      781 gcgttcctct acatcgatca ctgcctgatc aaggcgcaca aagtgatctc cgggctggtg
      841 atcaaccgcg agaagatcgc gcgagacctc cgaacgcatg gcgatttcgc tggcacgggc
      901 cggcttctgg tgcgcctcca gcagatgggg gtgccccgcg aagaggccta ccgcatgatc
      961 cagagctgct cgctccaggc gcaggaggcc gtggtccgcg gcgaggagaa ccccctccga
     1021 gggcttcttc tggaggccat ccacgcgctc ccctatcccg aggcgcgcac gctgacggcg
     1081 gaggagattg ctacgctcct ggatgcgtcg gcctacgtgg gtggctcgtt cgagctcacc
     1141 gaggagtttc tgtcggtctt gcgcggcgcc ctcagcgcct acccggaggt ccagcaggac
     1201 gccagcgaga gcgttttctg aaagctggga tcccgagata aggctcagat ggttgcctgc
     1261 gttctcccgc cagcgcgcca ccttcgatac cacggatgag ggggccggac acggatgggg
     1321 agggccgccc gcatgcgcgg agccgaggct ccgcgctgct tccgggcaaa gcccctttgg
     1381 ggcttcccct cccaggccct gcccttacca gttgcccggg aatacagcca tgccgagccc
     1441 ggctgggctt cgctcgcaag cagcgcggtg atttatcgcc gcgctgcaca tgcacacccg
     1501 tgcgccgtcg cgcccggcgg ccccgcgcgt caactcaggg tgtcagggag atcccctcgc
     1561 ggagtggacc tgccgggttg gtgggcacgc cgtgaatgcg cagttcccag tgcaggtgcg
     1621 gcccggtaga gaaccccgtt gaccccacca gacctaccac ctcccccttc tttatctgct
     1681 ggcctggggt tacggagaag cgggagaggt gctcgtagag agtgaaaacc cccatgccgt
     1741 ggtcgatagc cacggtgttg ccgcgcatcc acagcttccg ggcgagggtg acccggccgg
     1801 tgttggcggc ctttaccggc cggcccgcgg gggacgcgac gtcacgcccg gagtggaacc
     1861 cccagaaacg cccgttcaca taccgctcgc gcccaaaggg cgaggtgatg cggccttcga
     1921 gtggattggc aaaggggccc tcccatagcg gctccggtgc cgtgctcgcc agagcctcgt
     1981 tgaggatctc tcgctccttc ttcaggattt ccggagacat cagccccgtc ttgctctttg
     2041 ccatgcggat gcgctgggtg gggaaattgg cggaggtcac tgtgagagac agggggaact
     2101 tctcatggct gccgtcctga tagcgcaccc agagcgtggc ggtaccctcg ccggggcgcg
     2161 tgcgggcgct cacgccgagc aggccggtga ggacgccctg ataaggaaag c
//
LOCUS       DAOSCV010000789         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32571_length_2202_cov_3.331626, whole genome shotgun sequence.
ACCESSION   DAOSCV010000789 DAOSCV010000000
VERSION     DAOSCV010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32571_length_2202_cov_3.331626"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1012
                     /gene="rsmI"
                     /locus_tag="PLY56_16260"
     CDS             152..1012
                     /gene="rsmI"
                     /locus_tag="PLY56_16260"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ23082.1"
                     /translation="MAVSSESGTLFVVATPIGNLEDITLRALRVLKEADLIAAEDTRV
                     TRKLLAHFDIHTPLTSYHQHTEARKVEWIVEQVAEGRRVALVSDAGTPGISDPGDELI
                     TACIEAGLDVVVIPGPSAVIAALVASGLPTRRFAFEGFLPRAAGERRVRLEALRAEER
                     TLLFYEAPSRVLATLEAICQVLGDRRVAVAREMTKRFEEVVRGRASEVLEHFREHPPQ
                     GEFVLVVGGATPGEAPPPAEPAVSPEEDVAERVAAGMSERDAIRAVATERHLPRREVY
                     AAWLQHKRGG"
     gene            complement(1160..1609)
                     /locus_tag="PLY56_16265"
     CDS             complement(1160..1609)
                     /locus_tag="PLY56_16265"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOJ23083.1"
                     /translation="MAASAKPARVLTTTAILSSDEKEFVRLVNEERARRGLRQLTVDP
                     VLTAAARQHSREMAELGYFDHYSPVRGLRSPIERYAVALGSRRFTCAVGENLFYSSRR
                     DVALGHRSLMNSPGHRANILASDYRAIGVGVYQAPSGEYYATQMFHS"
BASE COUNT          357 a          661 c          795 g          389 t
ORIGIN      
        1 aaagcagcgc ggagcttcga gctccgcgcg tgcgggcagc cttctcctta tctcgaaatc
       61 ccaggggctt gggcggtagc gttgacgcga ccgggggggc atggtagaat ggccaggctg
      121 gccggatccg cgccggccga gaggggaact cgtggcagtg agtagcgaat cgggaacgct
      181 tttcgtcgtc gcgacgccga tcggcaatct ggaggacatc actctgcgcg cgttgcgcgt
      241 tctgaaggag gcggatctga tagccgccga ggatacccgc gtcacacgca agctcctcgc
      301 gcacttcgac atccacacgc cactcacgtc gtaccatcag cacacggagg cgcgcaaggt
      361 ggagtggatc gtggagcagg ttgcggaagg ccggcgcgtg gctctggtct ccgatgccgg
      421 aacccccggc atctccgatc ccggagacga gttgattacg gcttgcatcg aggcgggcct
      481 tgatgtggtc gtcatccccg ggccgagcgc cgtcatcgcg gccctcgtcg cttccgggtt
      541 gccgacgcgc cgctttgcgt ttgagggctt tctcccacgc gctgcgggcg agcgccgggt
      601 ccgcctggag gcactccgcg ccgaggaacg cactctcctc ttctatgagg ccccctcgcg
      661 cgtcctcgcc acgctcgagg cgatctgcca ggtgttggga gaccggcggg tggccgtggc
      721 gcgcgaaatg accaagcgct tcgaggaggt cgtgcgcggg cgggcaagtg aagtcctgga
      781 gcatttccgt gaacatccgc cccagggcga attcgttctc gtggttggcg gcgcgacgcc
      841 cggcgaggcg cccccgccag cggaaccggc ggtgtccccg gaagaggatg tggcggagcg
      901 ggttgccgcg ggtatgagcg agcgtgatgc catccgcgcg gttgctaccg agcgccatct
      961 ccctcgacgg gaggtgtacg cggcgtggct tcagcacaag cggggcgggt agcaagcgag
     1021 ggcacggttg tccgcttcga tgctcccggc tcggtttggt gagaaggcga gggagcggga
     1081 agcacggaaa gcatccgggg ggatgtgccg gggccttcag tgcttcccgg tcgggtgtgt
     1141 gctctcgcag ggtccattct caggagtgga acatctgcgt ggcgtagtac tcgccgctcg
     1201 gcgcctggta gaccccgacg ccgatggcgc ggtagtcact ggccaggatg ttggcgcggt
     1261 gccccgggct gttcatcagc gagcggtgac caagggcgac atcgcgccgc gagctgtaga
     1321 aaaggttttc acccacggcg caggtgaaac gccgggaacc cagggcgacg gcatagcgct
     1381 cgatcggtga gcgcagtccg cgcacgggcg aatagtggtc gaagtagcct agctccgcca
     1441 tctcgcggct gtgttggcgt gccgcggccg tgagcaccgg atcgaccgtc agctggcgga
     1501 gaccccgtcg cgcccgctcc tcgttcacaa ggcggacgaa ttccttctca tcactggaga
     1561 gaatggcggt cgtcgtcagc acgcgagccg gctttgccga cgcggccaat cggctgctgc
     1621 gataggtctt gacactcgcg cgccgtggat tcctggcggc ggttcttgcc ttgcgccggg
     1681 cttgcgtcca cgcagagggg tacgcgaagg agctccccag gcggggtgtg agggggtgat
     1741 tccgcctggc gtcccgagtt gggcttgcgg tggcatgtac cagtggaagc gcgagaagga
     1801 tggcgaaaag ggatgacgct cgcttcacag tggcggagac tcctttcaac caggcccacg
     1861 cgcccccgat gtgccgccga acgatggcgt tcttgggacg gcactctgtc cgatggaagc
     1921 gtggtggccc cgcgcgggtt gttgggattg cctgcagcac ctcacgcggc ggtgttgtga
     1981 cttggttgcg gcctgtattc ttaggacgaa aagaaacacc ctcaggttcg catgacggca
     2041 agatcaggag gcatgggcac gcatgcttga ggtgctcctc tggtctacga gggctgagtc
     2101 tgtttggcca acgccccttg acagtcgatc aggaaagggg gtacaatccg ggtgccaata
     2161 gacactagcg accgcagcgc gccgcgaagg atgatgccgt ga
//
LOCUS       DAOSCV010000790         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32573_length_2202_cov_3.176525, whole genome shotgun sequence.
ACCESSION   DAOSCV010000790 DAOSCV010000000
VERSION     DAOSCV010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32573_length_2202_cov_3.176525"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLY56_16270"
     CDS             complement(<1..274)
                     /locus_tag="PLY56_16270"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23084.1"
                     /translation="MAGTPVKIGIIGAGAIGQRRHIPEAIANPDCTLVAVCDANAERA
                     KEVAAKNGVKAYTDYKQMLETEKLDAVVVCGPNTLHAQQTLDALKAG"
     gene            complement(431..1591)
                     /locus_tag="PLY56_16275"
     CDS             complement(431..1591)
                     /locus_tag="PLY56_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23085.1"
                     /translation="MAKKVNVALIGYKFMGKAHSNAFRQAPVFFDLDVEPVMKVLVGR
                     DEAGVKAAAEKLGWESYATDYREVVNRPDIDLVDIVTGNTSHAEIAIAAAKAGKHVFC
                     EKPLAMTVGEAREMTQVAEKAGVKHCVNFNYRKLPSIQLAKRLIDEGAIGRIFHWRSV
                     YLQDWIVDPEFPLVWRLNKAEAGSGALGDLAAHSIDLAHFLVGDITAVTGHMETFIKE
                     RPVATSMSGLVATGSEERAPVTVDDGTIFLARFANGAIGTFEATRFAPGHRNGNMFEI
                     NGSEGSIRFNVERLNELEFYSRRDPAHAQGFRTISVTEGGEHRWINAWWPAGHIIGWE
                     HSFTHQVVDLLDAIAQDKPATPDFRDGLRVQLVLDAVERSAQSGQWVSIEKE"
     gene            1878..>2202
                     /locus_tag="PLY56_16280"
     CDS             1878..>2202
                     /locus_tag="PLY56_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23086.1"
                     /translation="MRKSTPQAPAVPLIRLGIACLLGLFMAVAPCRADAVPEGAPGPP
                     AANAEAVLPPDAGRKPDGSQERPAASKAPAEQNAPPKQAGARKEDEEVQLGRRAAAML
                     EREYRL"
BASE COUNT          375 a          723 c          707 g          397 t
ORIGIN      
        1 agccggcctt gagcgcgtcg agcgtctgct gcgcgtggag cgtgttgggc ccgcagacga
       61 ccaccgcgtc cagcttctcg gtctcgagca tctgcttata gtcggtgtag gctttcacac
      121 cattcttggc agccacttct ttggcgcgct ctgcattcgc atcgcacacg gccacgagtg
      181 tgcaatcggg gttggctatt gcctcgggga tatgccggcg ctggccgatg gcgcccgcgc
      241 cgatgatacc gatcttgact ggggtacctg ccatggggtt ctcctatctc tctatcacga
      301 agccacgagc caggccgtgg cagctccggt cccacgccct agctgccggc cctcgcgcct
      361 tcgcatctgg aatctctatc gcgataaggt gatgggggcc aggcatgaac cgcatggccc
      421 cctcctgcta ctactctttc tcgattgaca cccactgccc gctctgagca gaacgctcca
      481 cggcatcgag aacgagctgc acgcgcaggc catcgcggaa gtcgggcgtt gccggcttgt
      541 cttgagcaat cgcatccagc agatccacga cttggtgggt gaagctatgc tcccacccga
      601 tgatatgccc ggcgggccac caggcattga tccagcggtg ttcgcccccc tccgtgaccg
      661 agatcgttcg gaagccctgg gcgtgagccg gatcacggcg agagtagaac tccagctcat
      721 tgaggcgctc gacgttgaag cggatgctcc cttcgctccc gttgatctcg aacatgttgc
      781 cgttgcgatg ccccggagca aagcgggtgg cctcgaacgt gccgatcgcg ccgttggcaa
      841 agcgcgccag gaagatcgtg ccgtcatcca cggtcaccgg tgcgcgctcc tcgcttccgg
      901 tggccaccaa gccgctcatc gacgtggcga cggggcgctc cttgatgaac gtctccatgt
      961 gcccggtcac ggcggtgatg tcgcccacga ggaagtgcgc caggtcgatg gaatgggcgg
     1021 cgaggtctcc gagagcgccc gagcccgcct cggccttgtt gaggcgccac accaacggaa
     1081 actcggggtc gacgatccaa tcctgcagat agaccgaccg ccaatggaag atgcggccga
     1141 tcgccccctc gtcaatgagg cgcttggcca gctgaatcga gggaagcttc cggtagttga
     1201 agttgacgca gtgcttgacg ccggccttct cggccacctg cgtcatctct ctcgcctcgc
     1261 ccaccgtcat cgcgaggggc ttctcgcaga aaacgtgctt gcccgccttg gcggcggcga
     1321 tggcgatctc cgcatgcgac gtgttccccg tcacgatatc gacgaggtcg atatccgggc
     1381 ggttgaccac ttcgcgatag tcggtcgcgt agctctccca gccgagcttc tccgcggccg
     1441 ccttcacacc cgcctcgtct cggccgacga ggaccttcat caccggttcc acatcgaggt
     1501 cgaagaacac aggggcctgt cgaaacgcat tgctgtgggc cttgcccatg aacttgtaac
     1561 cgatcagggc tacgttcacc ttcttggcca tggcaacggc tggcctcctt tctgtggtga
     1621 cagagggggg aagcggctga aaccgcgtcg cgtggattgt agcatactcc ccggcgtttg
     1681 taaagacgaa tgcacctcaa gcgggcaggc tttgggcgtt cggctggaaa cttgcagtcg
     1741 gctctccgaa tagcgagcgg acgccacccg atccgatggc ggacgcccga tggatggcga
     1801 gtccggccgg gccagatagg ggccggtcac tccgatcacc agcaggcggc tcgcggatcg
     1861 ggaagatgga ggagagagtg aggaagagca cgccacaagc gccggccgtg cctctgatcc
     1921 gcctgggaat cgcgtgtctc ttagggctgt tcatggccgt cgccccgtgc cgggcagatg
     1981 ccgtgcccga aggagcgccc ggtccgccgg ccgcgaacgc cgaagccgtg ctaccgcccg
     2041 atgcgggccg gaagcccgac ggcagccagg agcgcccggc ggcttccaaa gctcctgccg
     2101 agcagaacgc cccgccgaag caggccgggg cgcgcaagga ggatgaggag gtccagcttg
     2161 gccgacgcgc cgcggccatg ctggagcggg aataccgcct gg
//
LOCUS       DAOSCV010000791         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32687_length_2196_cov_4.453526, whole genome shotgun sequence.
ACCESSION   DAOSCV010000791 DAOSCV010000000
VERSION     DAOSCV010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32687_length_2196_cov_4.453526"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PLY56_16285"
     CDS             complement(<1..545)
                     /locus_tag="PLY56_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HOJ23087.1"
                     /translation="MRSIALFLIALAIAAPAQAAPSIEQLVERAFRHPRSVAHTGTVR
                     VTLSGPPRSTASVRVFCDGHGRERREHLDGPARGLTILTDGQSSWQRPSPAARWTALQ
                     IPPATSSWDRLRRNYRFRRQGEERIAGRKAAVIAIDPVHPGNPRQVLWLDMATALVLR
                     ADSYTYAGDLAMTTHYTTFVP"
     gene            complement(542..1027)
                     /locus_tag="PLY56_16290"
     CDS             complement(542..1027)
                     /locus_tag="PLY56_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ23088.1"
                     /translation="MAESRQESGASRCDRVREWLPEFDYGGPGLWQRWQVARHLKRCP
                     ECARELETLRRAVALIESIPEVSPPADLWERVAGELRREGQSASSTRVMRQLPALTAA
                     AVLAASAGLFVWHRPEPAVAPQPMASYVRSHLALSSSQPFAPSAGLDALVLISAGEDA
                     P"
     gene            complement(1031..1597)
                     /locus_tag="PLY56_16295"
     CDS             complement(1031..1597)
                     /locus_tag="PLY56_16295"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ23089.1"
                     /translation="MPESEETTVRRAQAGDRDAFRCLFERYQNQVYGYVRRMVSEENL
                     AADLAQDAFVRAYEALPRLKAPGAFRGWLFQIAANRVRDHWKRPCRDALSLDAPAAET
                     GGELAVPDPGPGPERLAVREQMRTSVAEALAKLPPERREVVVLHHLNGLDVRTIAAML
                     GVPEGTVKSRLGRARADLRQLLAEWMEE"
     gene            1870..>2196
                     /locus_tag="PLY56_16300"
     CDS             1870..>2196
                     /locus_tag="PLY56_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOJ23090.1"
                     /translation="MEDLTQRALDRARQAGASYADVRVVRRTTEEILVKNGHVEAVEA
                     GESTGFGVRVVVDGAWGFASSRQVDADEMERVAELATRIARASALVQREEVDLAPVEP
                     ARGRFEM"
BASE COUNT          350 a          725 c          784 g          337 t
ORIGIN      
        1 ttgggcacga aggtggtgta gtgcgtggtc atcgccaggt cgccggcata ggtgtagcta
       61 tcggcgcgca acacgagcgc cgtcgccatg tcgagccaga ggacctggcg cgggtttccc
      121 gggtggaccg ggtcgatggc gatgacggcc gctttccggc cggcgatccg ctcctcgccc
      181 tggcggcgaa aacgatagtt gcgccggagg cgatcccaag acgaggtggc ggggggaatc
      241 tggagggcgg tccagcgcgc cgcgggcgaa ggccgctgcc aggagctctg gccatcggtg
      301 aggatggtga gcccgcgtgc cgggccgtcc aggtgctcgc gtcgttcccg gccatgaccg
      361 tcgcaaaaga cgcggaccga tgccgtcgag cgaggcgggc cgctgagcgt gacgcgtacc
      421 gtaccggtgt gggccacgga gcgcgggtgg cggaaggcgc gctccaccag ctgctcgatg
      481 gagggcgcgg cctgcgccgg cgcggcaatc gcaagggcta tcaggaagag cgcgatggac
      541 ctcatggcgc gtcctctccg gcgctgatca ggaccagcgc atcaagcccg gcgctcgggg
      601 cgaatggttg cgagctcgaa agcgccaggt gggatcgcac atagctggcc atcggctggg
      661 gcgccaccgc cggctcggga cggtgccata cgaagaggcc tgccgaggcg gcgagcacgg
      721 cagcggcggt gagcgctggc aactggcgca tcactcgcgt ggaggaagcg gactgcccct
      781 cgcgccggag ctcgcccgcg acgcgctccc acaagtccgc cggcggggag acctcgggaa
      841 tggactcgat cagggccacc gcgcgccgga gcgtctccaa ctctcgcgcg cactccgggc
      901 agcgcttcag gtgccgcgcc acctgccacc gctgccagag gccaggcccg ccatagtcga
      961 attccggcag ccactcccgg actcggtcgc agcgcgaggc gccgctctcc tgtcgggact
     1021 ccgccatctt ctattcctcc atccattcgg ccagcaattg ccgcagatcg gcccgggcgc
     1081 gtccaagccg cgatttcacg gtgccctccg gcactcccaa catcgctgca atcgtacgca
     1141 cgtcgaggcc attcaagtga tgcagcacca ccacttcgcg gcgctccggc ggcagcttcg
     1201 cgagcgcctc cgccaccgag gtgcgcatct gctcacggac ggcgaggcgc tccgggccgg
     1261 ggcccgggtc tgggacggcc agttcgccgc ccgtttcagc agctggcgcg tccaggctga
     1321 gcgcatcccg gcaaggccgc ttccaatggt cgcgcactcg attggcggcg atctggaaga
     1381 gccagcctct gaacgctccc ggcgccttca gacgtggcag cgcctcgtag gcgcgcacga
     1441 aggcatcctg cgcaaggtca gcggcgagat tctcctcgct caccatccgg cgcacatagc
     1501 cgtacacttg attctggtag cgttcgaaga ggcaccggaa cgcatcccgg tcgcctgcct
     1561 gcgcgcgccg gacggtggtc tcttcggatt cgggcatctc gcgcgtgtct cccgatctgg
     1621 tctgtgtctc tcggcgccgc acacatccct tgtcactata caagacgagc tacccggcga
     1681 ggcgttcccg aagagcgtga gagatcccgt tcgcgccggc gcgaacggcc tgattggccc
     1741 ggatccggca gttacagccg cccgcgagca cgccgacaag aagcgtttgc cccaaatctg
     1801 agcgtatgtt ataatgcgcc ctgggtggga agagacccag gtgcgttctc atagcgaggg
     1861 gaggcgccga tggaggattt gacacagcgc gcgctggatc gggcccggca ggcgggggct
     1921 tcctatgccg atgtccgcgt ggtccggcgt acgactgagg agatcctggt caaaaacggg
     1981 cacgttgagg cggttgaagc gggtgagagc accggctttg gggtgcgtgt cgtcgtcgat
     2041 ggcgcgtggg gatttgccag cagccggcag gtggatgccg acgagatgga acgcgtcgcg
     2101 gagctggcca cacggattgc ccgcgcgagc gctctggtgc agcgcgaaga ggtagacctg
     2161 gcccctgtcg agccggcgcg cgggcgcttc gaaatg
//
LOCUS       DAOSCV010000792         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32757_length_2193_cov_2.780636, whole genome shotgun sequence.
ACCESSION   DAOSCV010000792 DAOSCV010000000
VERSION     DAOSCV010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32757_length_2193_cov_2.780636"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLY56_16305"
     CDS             complement(<1..296)
                     /locus_tag="PLY56_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ23091.1"
                     /translation="MSQKRRFTKVQAAGNDFILFDCLQEPELPWSSLAPRLCDRHRGI
                     GSDGLLIVLPSMGANFRMRMFNPDGTEDFCGNGLRCVARYLHATGLHRQSELIL"
     gene            complement(293..>2193)
                     /locus_tag="PLY56_16310"
     CDS             complement(293..>2193)
                     /locus_tag="PLY56_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23092.1"
                     /translation="GINDHDYFSKTSAPLPTDAPFAILEHNDGTTHDLWVATGELSML
                     FGGETVLTRERLHGCGVEVEKVAKGCPEGREACIDRITAAWGWRGIAQTRDHRHIAHE
                     IRLSDVLPWLTEILEWGFRESAALLEGEDARESAERFGEEVVEWLRRFDHEHPGALLS
                     DAYQEAHRLFFRKLAGCDPERVTTFTSTDLFLFNRETVERPRFALLNLFLKPESREIA
                     CAAYDAAVEGSNTYTLDRFGEGAIPFDLVVPAGRGTLRILDDAVVVETPEPIRMPVPR
                     RIESARELAEVVEDHFGQNTTLIGKGHVFVCMVTAEAILVFHESGSAYVHRTARLMQT
                     LADRGFSVPLYPILRIRHHTWDALAGCGVRFRLPEHLAAAFGAPVVTATEFATRWQEV
                     VDAEKRLLQEISALSSPRELVSFLGARDDGEWLRRLEEYTRAHDLLLEIRDRSRVYEE
                     RSRQIYEEIQRLKSEALEMEREKGENFRRTIKPLRERLFELAQEGVVDGPEVEELQRQ
                     IEAHEAPRAHVDAEIRARRERVAALENEAKEVRKARMENEKGPAAVAARQAIAEVEKE
                     AERAKLQLVRRALLVSLGLPACNLRPTAWWFPLVDPSGEWFRNLAHRMELRFERLGPV
                     DPATGGGA"
BASE COUNT          365 a          764 c          709 g          355 t
ORIGIN      
        1 tccaggatga gctcgctctg gcggtgcaga ccggtcgcgt ggagatagcg cgcgacgcag
       61 cgcaggccat tcccgcagaa atcctcggtg ccgtccggat tgaacatgcg catccggaaa
      121 ttcgccccca tcgaggggag cacgatcagc aggccatccg aaccgatgcc gcggtgacgg
      181 tcgcacagcc ggggggcaag cgaggaccac ggaagctccg gctcttgcag gcagtcgaag
      241 aggatgaagt cgttgccggc ggcctggacc ttggtaaagc gtctcttctg gctcacgcgc
      301 cgcctccggt ggccgggtcc acggggccga ggcgctcgaa gcgcagctcc atccggtgcg
      361 ccaggttccg gaaccactcg ccgctgggat cgaccagggg gaaccaccac gccgttgggc
      421 gcaggttgca cgcgggcagc ccgagcgaga ccaagagggc acgacgaacg agctggagtt
      481 tggcgcgctc tgcctccttc tccacctcgg caatcgcctg gcgcgcggcc actgccgcgg
      541 ggcccttctc gttctccatg cgcgccttgc gcacctcttt cgcctcgttc tccagggcag
      601 ccacgcgctc gcgccgcgcg cgaatctccg cgtccacgtg cgcccgaggc gcctcgtgcg
      661 cctcaatctg gcgctgcaac tcctctacct cgggcccatc aacgacgccc tcctgagcca
      721 gctcgaagag gcgctcgcgc aacggcttga tcgtccgccg gaagttctcg cctttctctc
      781 gctccatctc cagggcttca ctcttcagcc gctggatctc ctcgtagatc tgccggctgc
      841 gctcttcgta gacgcgcgag cggtcgcgga tctccaggag gaggtcgtgt gcccgcgtgt
      901 actcttccag gcgccgcagc cactcgccat cgtctcgggc accgaggaac gagaccaact
      961 cgcgcgggct cgagagcgcc gagatctcct ggagcaggcg cttctccgcg tccaccacct
     1021 cctgccagcg cgtcgcgaac tccgtcgccg tgaccaccgg cgccccaaaa gcggcggcca
     1081 ggtgctccgg cagccggaac cggacgccgc atccggcgag ggcgtcccag gtgtggtgac
     1141 ggatccgcag gatcgggtaa agcggcaccg agaacccgcg atcggcgagg gtctgcatca
     1201 ggcgcgccgt gcgatgcacg taggcagagc cgctctcgtg gaacaccagg atcgcctcgg
     1261 cggtcaccat gcagacgaaa acatgtccct tgccgatgag cgtcgtgttc tggccaaagt
     1321 gatcctcgac cacttccgcc agttcgcgcg cgctctcgat gcgcctaggc accggcatcc
     1381 ggatcggctc cggggtctcc accaccacgg catcgtccag gatgcgcaag gtaccccgtc
     1441 ccgccggcac caccagatca aacggaatgg cgccctcgcc aaagcggtcg agcgtgtagg
     1501 tgttgcttcc ctcgacggcg gcatcgtagg cggcacaggc gatctcgcgc gattccggct
     1561 tcaggaagag gttgaggagg gcgaaccgcg ggcgctctac ggtttcgcgg ttgaagagga
     1621 agaggtcagt ggaagtgaag gtggtcacgc gctccggatc acatccggcc agcttgcgga
     1681 agaagagccg gtgcgcctcc tgataggcat cggagaggag tgcgcccggg tgctcgtggt
     1741 cgaaacggcg gagccactcc accacctcct cgccaaagcg ttcggccgac tcgcgcgcat
     1801 cctccccctc tagcagggcg gcactctcgc ggaagcccca ctccaggatc tcggtcagcc
     1861 acggaagcac gtcgctcagg cgaatctcgt gggcgatgtg ccggtgatcc ctcgtctgcg
     1921 cgatccctcg ccacccccaa gcggcggtga tccggtcgat gcacgcctcg cggccttcgg
     1981 gacagccttt ggccaccttc tccacctcaa cgccacaccc gtggaggcgc tcgcgcgtga
     2041 gcaccgtctc gccgccaaag agcatggaga gctcgccggt tgccacccac aggtcgtgcg
     2101 tggtgccatc gttatgctca aggatggcaa agggcgcgtc ggtgggcaaa ggcgcggatg
     2161 tcttcgagaa gtagtcgtgg tcgttgatgc caa
//
LOCUS       DAOSCV010000793         2188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32849_length_2188_cov_3.790436, whole genome shotgun sequence.
ACCESSION   DAOSCV010000793 DAOSCV010000000
VERSION     DAOSCV010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32849_length_2188_cov_3.790436"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLY56_16315"
     CDS             <1..403
                     /locus_tag="PLY56_16315"
                     /inference="COORDINATES: protein motif:HMM:NF014077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOJ23093.1"
                     /translation="AISVASYADDLSFAPAAAFARRLASEVLLHGLPPQTLLNVNVPG
                     VPEEAIAGVRVTRLGRRHYPENVVKRTDPYGRVYYWLGGEPPEDVLEEGTDVKAIAEN
                     CISITPVQLDLTDYDLIPEVSTWNVVGTLKS"
     gene            476..1936
                     /gene="rpoN"
                     /locus_tag="PLY56_16320"
     CDS             476..1936
                     /gene="rpoN"
                     /locus_tag="PLY56_16320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOJ23094.1"
                     /translation="MKLQQRQEQRMVQKIDPRMIMANSMLQLSAMELMQLIDQELSDN
                     PALEVEEDPLCPRCNAPLDGPRCKNCGYSLREPTDEVPELLAFEYGGGGSFDEEEYDP
                     FARVEAVMTVQEHLRFQLRALASRQDYPIGECIISYINDDGYLEGWNPADVAEACHVD
                     EAKVEEVLHQVQSLDPPGVGARSLQECLLIQLRSDSLVEDANLESLEEREEFLALRDI
                     ALLMIERCWDELCAQKYPDIARRLKMDLEDAREAHAFIQRHLNPYPGRAFRPDWAPNV
                     TSSGEYVKPDVVVRRNGDNYEVLVVESNRFVLRVNESYRAACREMQRNKERYSEEERQ
                     HTAQYVERAELFIRNLNQRKRTLHTISTSLVDYHIGYFDTGDVTKLRPLTRGKLAALL
                     GVHESTVSRATANKYMQLPWQEVKGFDFFFDSSLSVKELIREIIDSEDPHNPYSDQAI
                     ADLLVKRYRVEIARRTVEKYRDEMKILSSRKRRKRQ"
BASE COUNT          427 a          697 c          667 g          397 t
ORIGIN      
        1 cgcgatctca gtggcgtcct acgcggatga tctctccttc gcgccggccg cggcgttcgc
       61 ccggcgtctc gcgtccgagg tcctcctgca cgggttgccc ccgcagacgc ttctgaatgt
      121 gaacgttccc ggcgttcccg aagaggcgat tgcgggggtt cgcgtcacgc gcctgggtcg
      181 ccgccactac ccggagaacg tcgtcaagcg caccgacccc tacggccggg tctactactg
      241 gctgggcggt gagccgcccg aggatgtgct ggaagaaggc accgacgtga aagcaatcgc
      301 agagaactgc atctccatca cgccggtgca gctcgatctg actgactacg acctgatccc
      361 ggaggtgagc acctggaatg tcgtcggcac tctgaagagc tgaacgggta ttccgcttcg
      421 tgccggcggc agtcagcccc atgtaaactg aaaacacggc cataggaggg ttgctgtgaa
      481 actgcaacag cggcaagagc agcgcatggt gcagaagatc gacccgcgga tgatcatggc
      541 gaacagcatg ctccagctct cggcaatgga gctgatgcag ctgattgacc aggagctatc
      601 cgacaacccc gcgctcgagg tggaggaaga cccgctctgc cctcgctgca atgcccccct
      661 cgatggcccc cgctgtaaaa actgcggcta ctccctccgc gagcccacag acgaggtccc
      721 cgagctcctc gccttcgaat acggcggtgg cggttccttc gatgaggagg aatacgatcc
      781 attcgcgcgt gtcgaagccg tgatgaccgt tcaggagcac ctccgcttcc agctccgcgc
      841 gctggcctct cgccaggact acccgatcgg tgaatgcatc atctcctaca tcaacgatga
      901 tggctatctg gagggatgga accccgccga cgtggccgag gcgtgccacg tcgatgaggc
      961 gaaggtggag gaggtgctcc accaggtgca atcgcttgat ccgcctggcg taggcgcgcg
     1021 cagcctgcag gaatgcctcc tcatccagct tcgctcggat tccctggtgg aagacgcgaa
     1081 cctggagtct ctcgaggagc gcgaggagtt tctggctctg cgcgacatcg ctctcctcat
     1141 gatagagcgc tgctgggatg agttgtgcgc ccagaagtat ccggatatcg cgcggcgcct
     1201 gaagatggat ctcgaggatg cgcgagaggc gcatgcgttc attcagcgcc acctcaaccc
     1261 ctacccgggc cgcgccttcc ggcccgattg ggcgcccaac gtcacatcca gcggcgagta
     1321 tgtgaagccg gatgtcgtgg tccgccgtaa cggcgacaac tacgaggtgc tcgtggtcga
     1381 gtccaaccgc ttcgtcctgc gggtgaatga gtcttaccgc gccgcgtgcc gcgaaatgca
     1441 gcgcaataag gagcgctatt ccgaggagga gcgccagcac acagcccaat acgtggaacg
     1501 cgccgagctc ttcatccgca acctcaatca acggaagcgg acgctccaca cgatcagcac
     1561 ctccctggtg gattaccaca tcggctactt cgatacaggc gacgtcacga agctgcgtcc
     1621 gctcacgcgc ggcaagctgg ccgcgctcct gggagtgcat gaaagcacgg tgagccgcgc
     1681 caccgcgaac aagtatatgc agcttccctg gcaagaggtg aagggcttcg atttcttctt
     1741 cgattcctcg ctctcggtaa aggagctgat acgcgagatc atcgattcgg aggatccaca
     1801 caatccttat agtgaccagg ccatcgccga tctgctggtc aagcgctacc gcgtggagat
     1861 cgcgcggcgc accgtcgaga aataccgtga cgagatgaag atcctctctt cacgcaagcg
     1921 acgcaagcgg caatgagcgg cctcagccct ccgcggcccc ccggacggcg gtatgcgccg
     1981 gcgccccttt ttccggggac cagatttctg ttcctgtttg agcaaaaata tgatatgatg
     2041 ttgctggcag acaagacggt ggtggcacgc ctgcgtcggg tgtaaccgca gacgcggcac
     2101 tgcagaagaa cgcctcggtc ctgccgcacg cgagcgcgtg ccctcttgct ggcttcacga
     2161 ctattccggc gctgccggcg ttgccata
//
LOCUS       DAOSCV010000794         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32873_length_2187_cov_3.296435, whole genome shotgun sequence.
ACCESSION   DAOSCV010000794 DAOSCV010000000
VERSION     DAOSCV010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32873_length_2187_cov_3.296435"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLY56_16325"
     CDS             complement(<1..180)
                     /locus_tag="PLY56_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23095.1"
                     /translation="MRTATEGQRARRVKWTAVAVVAVAIIAAAIVWRLRPEGVAVVLP
                     QVEPVVESIAATGRVA"
     gene            584..1822
                     /locus_tag="PLY56_16330"
     CDS             584..1822
                     /locus_tag="PLY56_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23096.1"
                     /translation="MRRSRLGLISSALAVKWQFLSPRLAGRAQAICLTLVLLAPGAAT
                     ALAAEAEVAVDDSGVRSRVSTSQGHAGHRWDERTFNLNSGAARYAFRYSACVDPSHGD
                     SRPAGEGTLGMPVPSSTNWYHSGFLFLSINGKEATAFPLSDSRVLEKGARGSFQMVFE
                     HPDAVVGLRALMLPGANHVLMDLTWTPRTPGAVKSASLKAICYPSFFTSWHKRKGDRH
                     CMTPRIDEREPKTLELDPAADGYLYYYDTIFDVARGEGDGPCALIFTPEGVQSGQVNI
                     GNYAVITTLTLKPDAGRVRLAFYDFTGKKNAEAEAYLKAHGLEDRARLAEMDFRPEGV
                     RTLQVAQLEADAAKCLADAGEDGKPFQPKVTDLLTKVKELKAKADVNDWRAEAELAGL
                     LLDSQDLFWKLRIAALLNAP"
     gene            complement(1819..>2187)
                     /locus_tag="PLY56_16335"
     CDS             complement(1819..>2187)
                     /locus_tag="PLY56_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23097.1"
                     /translation="YAFAYYRDLFDFTRNARAAFSDDGVRQAADAVMQGIPGESGGPV
                     LYERHNLPAVMNSHGLSIYLPRLGEFYGSYDSLRFNRDFPNWSRLLHDTAGSSAGRVK
                     EQRAGHRSRSGSDTRAEGPL"
BASE COUNT          378 a          750 c          696 g          363 t
ORIGIN      
        1 ggccactcga cccgtcgccg cgatcgactc gaccacgggc tccacctgtg ggagtacgac
       61 ggccactccc tctgggcgca agcgccagac aatcgccgca gcgatgattg ccacggcgac
      121 cacagcgacg gcagtccact tcacccgccg cgcgcgttgt ccctccgttg cagttctcac
      181 tccggcgctc ccacacggcg atctgatgga gaaacgctcc ggcgcgtccg catcactgct
      241 gaagctccac cggctggtac gttcccatcg ccgtgacacg ccaaccgtcg gccacctgca
      301 gcctccacag accagaatcc agatgcccgg ctgctggcgt ggaggaagca gagacgtcgg
      361 aagagtggct cgtctttcgg agcggcagga gatcgggcaa tcgtgtcaaa gctccgctaa
      421 aggcaatccg gttcagaccg catctgccgg gtcctcactt tccgatgggg cgccccgcgc
      481 actggagcct gttgccggcg cgcgccgcga tcggatctct ggccgctggc ggtagcaagt
      541 cactcgccct acccggcggg tgcaccgcga aggagggtcc aaaatgcgtc gctcgagact
      601 cggtctcatc tcttccgcgt tggccgtgaa gtggcagttc ctctcgcccc ggcttgccgg
      661 tcgggcccag gctatctgcc tgaccctggt gctccttgcg ccaggcgccg cgaccgccct
      721 cgccgccgag gcggaggttg ccgtggacga ctccggcgtt cgctcgcgcg tctccaccag
      781 ccaggggcat gcagggcatc ggtgggatga gcgaacgttc aacctcaatt cgggagccgc
      841 gcgctatgcc ttccgctact ccgcgtgcgt ggatccttcg catggcgact cgcggcccgc
      901 aggagagggc accctcggca tgccggtgcc ttcgagtacc aactggtatc atagcggctt
      961 cctcttcctc tccatcaacg gcaaagaggc gaccgccttc ccgctcagcg acagccgggt
     1021 gctggagaag ggcgctcgtg gctccttcca gatggtgttc gagcaccccg atgccgtcgt
     1081 cggactgcgc gccctgatgc tccccggcgc caaccacgtc ctcatggacc tcacctggac
     1141 gccccggacg ccgggcgcgg tgaagagcgc ctctctgaag gcgatctgct atcccagctt
     1201 cttcacttcg tggcacaagc gcaaaggcga ccgccactgc atgacccccc gcatcgatga
     1261 gcgcgagccg aagacgttgg agctcgatcc cgccgcggat ggctacctct actactacga
     1321 caccatcttc gatgtggccc gcggtgaggg cgacgggccg tgtgcgctga tctttacccc
     1381 cgaaggggtg cagagcggcc aggtcaacat cggcaactat gccgtgatca ccaccctgac
     1441 gctcaagccg gacgccgggc gggtccgcct ggccttctac gatttcaccg gcaagaagaa
     1501 cgcagaagca gaagcctacc tcaaagcgca cgggcttgag gatcgggccc gcttggcgga
     1561 gatggacttc cgcccggaag gggtgcgcac tctccaggtg gcgcagctcg aagcggacgc
     1621 cgccaagtgc ctcgccgatg ccggcgagga tggcaagccg ttccagccga aagtcacgga
     1681 tctcctgacc aaggtgaagg agctgaaagc gaaggcagac gtgaacgact ggcgcgccga
     1741 ggccgagctc gcgggcctgc tgctcgactc tcaggatctc ttctggaagc tgcgtatcgc
     1801 cgcgctcctg aacgcgcctt agagtggccc ctccgcgcga gtgtcgctgc cagacctgga
     1861 gcgatggcct gcccgctgct ccttgacgcg gccggcggaa gagccggccg tgtcatgcag
     1921 taggcggctc cagttgggga aatcgcggtt gaagcggaga gagtcgtagc tgccgtagaa
     1981 ctcgcccagc cgggggagat agatggagag cccgtgtgaa ttcatcaccg cggggaggtt
     2041 gtgtcgctcg tagagcacgg gtccgccact ctctccgggg atgccctgca tcaccgcgtc
     2101 tgccgcctgg cgcacgccgt catccgaaaa agccgcccgc gcgttgcgcg tgaaatcgaa
     2161 caggtcacgg tagtaagcaa acgcgta
//
LOCUS       DAOSCV010000795         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32876_length_2187_cov_3.117261, whole genome shotgun sequence.
ACCESSION   DAOSCV010000795 DAOSCV010000000
VERSION     DAOSCV010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32876_length_2187_cov_3.117261"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..709
                     /gene="recR"
                     /locus_tag="PLY56_16340"
     CDS             113..709
                     /gene="recR"
                     /locus_tag="PLY56_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ23098.1"
                     /translation="MEYAKSLARLIGQLERLPGVGPKSAQRMAFWILSHPREDAAALA
                     EAILEVKERIRLCPVCFNFTDEEICAIDRDPRRDRSLLCVVAETRDLVAMEKTNEYKG
                     VYHVLQGVISPMDGIGPELLKIRELIRRLEDGQVKEVILATNPTVEGDATALYLARLI
                     KPMGIRVTRIAHGLPVGGDLDYADQATLIQALEWRREI"
     gene            841..1746
                     /gene="pdxS"
                     /locus_tag="PLY56_16345"
     CDS             841..1746
                     /gene="pdxS"
                     /locus_tag="PLY56_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOJ23099.1"
                     /translation="MSDSNAALPEKSTWRTKVGLAEMLRGGVIMDVTTPEQAKIAEEA
                     GAVAVMALERVPADIRREGGVARMSDPLVIKKIMDAVSIPVMAKCRIGHFVEAQILEA
                     IGVDFIDESEVLTPADEQFHVDKFAFKVPFVCGARDLGEALRRIGEGAAMIRTKGEAG
                     SGNIVEAVRHMRAIMSGIRRIQTLREDELMAEAKSLGAPYDLVRYIHEHGKLPVPNFS
                     AGGVATPADAALVMQLGAEAVFVGSGIFKSDDPAPRAKAIVDAVTNFRRPDILAKVSE
                     GLGRAMSGIDVRQLDEKDQLATRGW"
     gene            1747..>2187
                     /gene="pdxT"
                     /locus_tag="PLY56_16350"
     CDS             1747..>2187
                     /gene="pdxT"
                     /locus_tag="PLY56_16350"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOJ23100.1"
                     /translation="MRIGVLALQGNFREHLAMLAHCGVQGSEVRTVEELAATDGLIIP
                     GGESTTVGKLMVRYGLDAAIRERVARGMPVFGTCMGLILIAREIEGSDQPCLGLMDVT
                     VRRNAFGRQVDSFECDLEVAGFAPPPVRAVFIRAPAISSVGADVE"
BASE COUNT          380 a          666 c          760 g          381 t
ORIGIN      
        1 tcgccaaggc ccaaaaggtg cgcgaggaga agctcggctc gctcatgtcc ggcctcccga
       61 agatccccgg cctctccttc tagggcgcga cagacaagcg agggggggac ggatggagta
      121 cgcgaaatca ctggcgcggt tgatcgggca gctagagcgc ctcccgggcg ttggcccgaa
      181 atccgcgcag cgcatggcgt tttggatact gagccacccg cgcgaggatg ccgccgccct
      241 cgcagaggcg atcctggaag tgaaagagcg catccgcctt tgtcccgtct gcttcaactt
      301 caccgacgag gagatctgcg ccatcgaccg ggatccccgg cgcgaccgct ctctgctctg
      361 cgtggtggca gagacgcgcg acctggtggc gatggagaag acgaacgagt ataagggtgt
      421 ctaccacgtg ttgcagggcg tgatttcgcc gatggatggc atcggcccgg agctgttgaa
      481 gatccgcgag ttgatccgcc gcctcgagga tggccaggtg aaggaggtga tcctggcgac
      541 aaatccaacg gtggaggggg atgccacggc gctctatctc gcccggttga tcaagcctat
      601 gggcatccgc gtcacgcgca tcgcccacgg gctgcctgtc ggcggcgacc ttgattacgc
      661 ggatcaggca acgctgatcc aggcgctgga atggcggcgc gagatctgag cgccgggcag
      721 ggtgtggtat cgtagggcca ttgaaatacg gaaaggggga tgcctggcac ctcgtcgcgt
      781 gtctccccta tttcccaaat caatgcttgt gtgttcctaa tcagggaggg ttagcaacca
      841 atgtccgata gcaatgctgc tttgccagag aaatccacct ggcgcaccaa ggtggggttg
      901 gccgagatgc tgcgcggcgg cgtgatcatg gatgtcacca cgccggagca ggcgaagatc
      961 gcggaagagg ccggtgccgt ggcggtcatg gcgctggagc gcgttcctgc cgatatccgt
     1021 cgcgaaggcg gcgtggcccg catgagcgac ccgttggtca tcaagaagat catggacgcc
     1081 gtctcgatcc ccgtgatggc gaagtgccgc atcggccact tcgtcgaggc gcagatcctg
     1141 gaggcgatcg gcgtcgattt cattgatgag agcgaagtgc tcacccctgc ggatgagcag
     1201 ttccacgtag acaagtttgc cttcaaggtg cctttcgtct gcggcgcgcg cgacctgggc
     1261 gaggctctgc gccgcatcgg cgagggcgcg gcgatgattc gcaccaaggg cgaggccggc
     1321 agcggcaata tcgtcgaggc cgtgcgccac atgcgcgcca tcatgtccgg tatccgccgc
     1381 attcagacgc tgcgggaaga tgagctgatg gccgaggcca agagcctcgg agcgccctat
     1441 gatctggtgc gctatatcca cgagcatggc aagctgccgg tgcccaactt ctccgcgggc
     1501 ggcgtggcga cccccgccga cgctgcgctc gtgatgcagc tgggcgccga ggcggtcttc
     1561 gtcggctcgg gcatcttcaa gagcgacgat cccgcgccgc gcgcgaaggc gatcgtggat
     1621 gcggtcacga atttccgccg ccccgatatc ctggcgaagg tctccgaggg cctcggccgc
     1681 gcgatgtccg gcatcgacgt gcgccagctc gacgagaagg atcagctcgc gacccggggg
     1741 tggtaagtga gaatcggcgt tctcgccctg cagggaaact tccgcgagca cctcgccatg
     1801 ctggcgcact gcggcgtcca gggcagcgaa gtgcgcactg tcgaggagtt ggcggcaacg
     1861 gatggcctga tcatcccggg tggcgaaagc actaccgtgg gcaagctgat ggtccgctac
     1921 ggcctggatg cggcgatccg cgagcgcgtc gcccgcggaa tgccggtttt cggcacctgc
     1981 atggggctta tcctcatagc gcgcgagatc gaggggagcg atcagccctg cctcggcctc
     2041 atggatgtga ccgtgcgccg caacgccttc gggcggcagg tcgactcctt cgaatgtgac
     2101 ctggaggtgg cgggcttcgc gccgccaccc gtccgcgcgg tcttcatccg cgcgccggcg
     2161 atcagttcag tcggcgcgga tgtcgag
//
LOCUS       DAOSCV010000796         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_32944_length_2184_cov_2.725693, whole genome shotgun sequence.
ACCESSION   DAOSCV010000796 DAOSCV010000000
VERSION     DAOSCV010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32944_length_2184_cov_2.725693"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="PLY56_16355"
     CDS             complement(<1..1707)
                     /locus_tag="PLY56_16355"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOJ23101.1"
                     /translation="MRNPGFTMAALLLASLLAGCGSGVDTGDSPTDPGEATLLVRSTP
                     AGATILVDGQPTGRVTPADVSAFAQSQESVVTLTLTGYHPWHGLARKMEGMTLTLDAT
                     LTPSSAGTGAIQLQSDPPGATVFLDNEQTAYTTPATIQNVPASQHVVEYRFAGFYPFR
                     EEVTVTPGRTASSTPVLTRLGQGRITGRVLDDQGNGVVGAQVGVRGLEVSTTTTVFGI
                     FVLPNVPQGTYTLDAALATGDTALIGSRPDVTVIAGVPTNSADIVMSRSGEAGSITGQ
                     VVDTAGRPIEGAEIFASVAVAPGFLPDSTVAPRRATTNSKGRYSLNALATGFWVVTAA
                     YPGYATVTRGISPQIYLSAGSTATVDFELPASSADTPHPPRQLGALAFTFPTTMTRSP
                     SAYEAIRDRILRRAWGSRYEKLRAQRQSVRGRSAPAGSLIEVDLSWSAPGDPDIIGYY
                     IGRSPRENTGYQLQDDLLNPHAGFWIDYDERLSPNTTYYYRVTAVNSKGLHSDPSNTA
                     QANPLGQIQVRGPINQAASATPRFEWEPLPGAFAYDVQVFANYPDRFVDPIWEAGSLA
                     ASG"
     gene            complement(1978..>2184)
                     /locus_tag="PLY56_16360"
     CDS             complement(1978..>2184)
                     /locus_tag="PLY56_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23102.1"
                     /translation="WTKGGGSYGMSTSPEDSLRLSAALGIEFSLNKYVNWQMDVRHIR
                     YTDANNAERNYKGNLLTGEVRARF"
BASE COUNT          362 a          679 c          769 g          374 t
ORIGIN      
        1 cccggaggcg gcgagtgatc cggcttccca gatgggatcg acgaagcggt ctgggtagtt
       61 ggcgaaaacc tgaacgtcat aggcgaatgc gcctggtagt ggttcccact cgaaccgcgg
      121 cgtggccgag gcagcctgat tgatcggacc gcgcacctga atctggccga gcgggttcgc
      181 ctgggccgtg ttgctcggat cggaatgcaa ccccttggag ttgaccgccg tgacacggta
      241 gtagtacgtg gtgttgggcg atagccgctc atcgtagtcg atccagaagc cggcatgcgg
      301 gttgagcagg tcatcctgca gctggtagcc ggtgttctcg cgagggctac gcccgatgta
      361 gtagccaatg atgtccgggt ctccgggggc ggaccatgag aggtccacct cgatgagcga
      421 gcccgcgggg gcagagcgcc cccggaccga ctgcctctgc gcgcgcagct tctcatatcg
      481 gctgccccag gcccggcgga ggatgcgatc gcgaatcgcc tcgtaggcgg agggggagcg
      541 cgtcatggtg gtcgggaaag tgaaagccag cgccccgagt tggcgcggcg gatgcggcgt
      601 atcggcgctc gaagccggaa gctcgaaatc gacggtggcg gtgctgcccg ccgacaggta
      661 aatctgcggg ctgatcccgc gggtgacggt cgcatacccc ggataggcgg cggtgaccac
      721 ccagaacccg gtggcgagcg cgttgagcga gtagcggccc ttggagttgg tggttgcgcg
      781 tcgcggggcg acggtggaat ccggcaggaa gccgggggcg actgccacgg acgcgaagat
      841 ctcggctcct tcaatcggcc ggcccgccgt atccaccacc tggcccgtaa tggatccggc
      901 ctctccactg cgcgacatca cgatatccgc gctgttcgta ggcactccgg cgatcaccgt
      961 cacatccggc cggctgccga tgagcgcggt atcccccgta gcaagcgcgg catcgagggt
     1021 gtaggtgcct tggggcacgt tcgggagcac gaaaatcccg aagacggtgg tggtggtcga
     1081 gacctccagc ccgcgcacgc cgacctgggc tccgacaacc ccgttgccct ggtcgtccag
     1141 cacccttccc gtgatacggc cctgccccag acgcgtcagg acgggcgtgg aagaggcagt
     1201 ccggcccggc gtgacagtga cctcctcgcg gaagggatag aaccccgcga atcggtattc
     1261 gaccacgtgc tgactcgcgg gcacattctg gatcgtggcg ggtgtcgtgt aggcggtctg
     1321 ctcgttatca agaaagacgg ttgctccggg cggatccgat tgcagctgga tggcgcccgt
     1381 tccggccgag gagggggtga gcgtcgcatc gagagtgaga gtcatcccct ccatcttcct
     1441 ggcgaggcca tgccacgggt ggtagccggt cagcgtgagc gtgacgacgc tctcctgcga
     1501 ctgcgcaaac gcagagacgt ccgcaggggt gaccctgccg gtgggctggc cgtccaccag
     1561 gatggtggcg ccggcggggg tagaccgcac cagcagcgtg gcttcgccgg ggtcggttgg
     1621 ggaatcgcct gtatccactc cgctgccgca tcctgccagc aagctggcaa ggaggagcgc
     1681 tgccatggta aagcctgggt tgcgcaacga gggacccctt cctgtgacag gtgaccgtac
     1741 acaccactgc cggtcgagcg gccgccgttg ggcttcatgc ctgcccgcgt ggcggccttt
     1801 tctcttggta gacggcgaat gcgcggtcaa tgtttcccag ctccctgaga gaccaggagt
     1861 aaccgcccgc gcttgccgat agagcgagcg aaggcgcgcc gggctcgcgc gccgccggct
     1921 tgtgacgatg tggccattcc ggcgcggcaa acagccgggt ggaagcgggg cgccggctca
     1981 gaagcgcgcg cgcacttcgc ccgtgagcag attgccttta tagttgcgct cggcgttgtt
     2041 cgcatcggtg taccggatat ggcgcacatc catctgccag ttgacgtact tgttcagcga
     2101 aaactcaatg cccaaggcgg ccgagaggcg cagcgaatcc tccgggctgg tgctcatccc
     2161 gtagctccca ccgccctttg tcca
//
LOCUS       DAOSCV010000797         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33052_length_2179_cov_4.227872, whole genome shotgun sequence.
ACCESSION   DAOSCV010000797 DAOSCV010000000
VERSION     DAOSCV010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33052_length_2179_cov_4.227872"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLY56_16365"
     CDS             complement(<1..1045)
                     /locus_tag="PLY56_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOJ23103.1"
                     /translation="MKLTGAEILVRALQAQGVEVLFGIPGAKTLHLHDALSRSPLRHI
                     LVRHEQGAAHAADGYARASGRVGVCLSTSGPGATNLVTGIATAQMDSIPLVAITCQVE
                     MDAIGTDAFQEADMTGITRSITKHNGLVSSVDRIEGAVAEAFHVASTGRPGAVLLDIP
                     SCVLKARGDYRGSATVDRVGYRPTLDGHPMQIRRAAEALAQAERPLLYVGGGVIASGA
                     SEEVRMLAERADLPVVCTLLGLGGFPGTHPLFVGMLGMYGSHAANKAVQHSDLILAVG
                     ARFDDRATGNAARFAPDARIIHIDIDPAEIGKNVRVDIPIVGDARRVVRQLLEAIKPC
                     DHGAWIETVDGYRR"
     gene            1837..>2179
                     /locus_tag="PLY56_16370"
     CDS             1837..>2179
                     /locus_tag="PLY56_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOJ23104.1"
                     /translation="MDVIQTPLHSGWEFAFADHGTIAGSADAAHLTFRPASVPGTNLT
                     DLQAAGLVEESTSATYEDSFAPYKSMDFVYRTGFEGGATAARRHVFLCFDGLDTVCEI
                     YLNGEKLAETEN"
BASE COUNT          383 a          728 c          702 g          366 t
ORIGIN      
        1 cccgccggta gccatccacg gtctcgatcc aggccccatg atcgcacggc ttgatggcct
       61 cgaggagctg gcgaacaacg cggcgcgcgt cgcccacaat ggggatatcc acgcgcacgt
      121 tcttgccgat ctcggcggga tcgatatcga tatggatgat gcgggcatct ggagcgaagc
      181 gcgcggcgtt tccggtggcg cggtcatcaa agcgcgcgcc gaccgccagg atcagatccg
      241 aatgctgcac agccttgttc gcggcgtggc tgccgtacat cccgagcatc ccgacgaaga
      301 gcgggtgggt gccggggaag ccgcccaggc caaggagcgt gcagacgacg gggagatccg
      361 cgcgctccgc gagcattcgg acttcctcac tcgctccaga agcgatgacg ccgcctccga
      421 catacagcag cgggcgctcg gcctgtgcca gcgcctccgc ggcacggcgg atctgcatcg
      481 gatggccatc gagggtcggc cggtaaccaa cgcgatccac ggtcgcggag ccacggtagt
      541 cgccgcgtgc cttcaggacg caggagggga tgtcgaggag cacggcgccc gggcggcccg
      601 tgctggccac gtggaacgcc tcggccacgg caccctcgat ccggtcgacg ctcgagacga
      661 ggccgttgtg cttggtgatc gagcgcgtga tgccagtcat gtcggcttcc tggaaagcat
      721 ccgtgccgat cgcatccatt tcgacctggc aggtgatcgc cacgagaggg atcgagtcca
      781 tctgcgcggt cgcgatgccg gtgaccaggt tggtggcgcc tgggcctgat gtcgagaggc
      841 agacacccac tcgcccgctg gcgcgggcat agccatccgc ggcatgggcc gcgccctgct
      901 cgtgcctcac gagaatgtgg cggagcgggc tgcgcgagag cgcatcatga agatgaagcg
      961 tcttggcacc tggtatcccg aagagaacct ccacgccctg cgcctgcagc gcacgcacca
     1021 gtatttccgc acccgtcagt ttcatgcaaa cctccctggt agaagcagcc ggggctcacc
     1081 gggcccgtga ccatctaccg ggaacccacc ggagaatcag aaaggccacg ggtctgcttc
     1141 ccgtggccct tggcattctg taatcgcgtg gagagtctat gctccgccgc cggccacggg
     1201 gatggctaca gcgacaacta cagccaccag ggcacccgtg actaggggga gcaagcacgc
     1261 gccgaactgc gccgctgccg gcgagctctc tgaaacagga tctctgtgcc gcgtgtccat
     1321 tgtctctcct ggctcattat agcccatgaa agcctggctg tcaacaatag gttagagtac
     1381 cgagataggg ctgaactgag cggttgcccg cgctcttctg gcggctttca tccccccggc
     1441 cccgcgccgg cggcgatatc acgaatgagg gggacggagg ccgataggga agcacgggtg
     1501 ccccggttct cacagccgcg ggcctgaaag cccgcgctgc ttcccggcaa aggcccgaag
     1561 ggcctttgac acccccacct tcgaccagcc tgccttgccc gcacgtagcc ctgcccagcc
     1621 cgcaggtggc tattgacacg caccgactcg gattgataag atcgcttcac cccaacctgg
     1681 gcagtgttgc actcccctta ccatgagcgt ttctcggcat gcccgagggc ttgtcgcggc
     1741 ccctcgcggt gagggaaaac gccgttctgc cggtccgcgg gcgagcgccc accgtgcccg
     1801 gccctgacct gtgtattgca acgggagcac tcgcagatgg atgtcattca aacccccttg
     1861 cattcgggat gggagtttgc cttcgcggac cacggcacga ttgccggctc ggcggatgcc
     1921 gctcacctga ctttccgccc ggcctccgtg ccaggtacca acctgaccga cctgcaggca
     1981 gccggcctgg tggaggagag cacctcggcg acctacgagg attccttcgc gccctacaag
     2041 agcatggatt tcgtctatcg gacggggttc gaggggggag ctaccgctgc caggcgccac
     2101 gtcttcctct gcttcgatgg cctggacacc gtctgcgaga tctacctcaa tggcgagaag
     2161 ctggccgaga cggagaact
//
LOCUS       DAOSCV010000798         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33096_length_2177_cov_3.858624, whole genome shotgun sequence.
ACCESSION   DAOSCV010000798 DAOSCV010000000
VERSION     DAOSCV010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33096_length_2177_cov_3.858624"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="PLY56_16375"
     CDS             complement(<1..2048)
                     /locus_tag="PLY56_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23105.1"
                     /translation="MRVTWLVLVALLFALPALAAPSAVETPEGVTLENGIVRAVFPAK
                     ANYVLADFRRVPDGENLINICILSYSVEGAQYWYQDNKTEPEYGLRPVTARVERGDDF
                     VRLVVSYPAEGEAKHFRFVKTHTLRAGSPVLENDYRLEAVGEISLRGGMSLPLIWFAP
                     RLSRGAVPSSGGSLLLGPGDAVRRDPGTPPWVAAYDPEKGEGIAFCLPDGQPQLGRNR
                     EYPAVSYSAPLGGKVAPQTEVKVTFHLAAFAGQQMAPALAAALSKAPGITVPARFRQV
                     YDLGGEHFDPADLSLWQKYRASVRRPIAFIKPADLERARENVRRHPWAKRLLAGYRGR
                     ADYVLNQPPEFVEQMIPALTPLCTLFTMCPHCEYAPVHGQYIWDVKRPDELKCRNCGT
                     VYPNEKYPEDLVLVARHGGEQRFTFYGGKSWNFNNIHLRSSWTGAIRAHKTGYMARAA
                     RDLAMVYALTGERAYGEKAAAILSRFATVYPGYLIHSGYGEIADMDPRVAAAKINNLP
                     ADELTCPPNKPDRRLQAGYWMAGRGFSAVGMEGIQLSQLVEAYDLVADLIPDAERQRI
                     EKDLFLEGTYLLLCDTALNNKTATNRSAVGQIGMAIGDPRRVRFGLAGLRWFINEWFL
                     PDGAACESPSYGNMTLSGIWQFADTLHGYSDPPGYQDAAGRIDNLDIYGEARYRSVFQ
                     V"
BASE COUNT          357 a          706 c          747 g          367 t
ORIGIN      
        1 acttggaaga cgctccggta gcgggcctcg ccatagatat cgaggttgtc gatgcgtccg
       61 gcggcatcct ggtagccagg cgggtcgctg tacccgtgca gggtgtcggc gaactgccag
      121 atgccggaga gggtcatatt gccgtaggag ggcgactcgc aggcggcgcc gtccggcagg
      181 aaccactcgt tgataaacca gcgcaatccg gccaggccga agcgcacccg gcgcgggtct
      241 ccaatcgcca ttccgatctg gcccacggcc gagcgattcg tcgcggtctt gttgttgagc
      301 gctgtgtcgc acaagagcag gtaggttccc tccaggaaga ggtccttctc gatccgctgg
      361 cgctcggcat ccgggatgag gtctgccacg aggtcgtagg cctccaccag ctgcgagagc
      421 tggatgccct ccatgcccac ggcggagaag ccgcgcccgg ccatccaata gcccgcttgc
      481 agccggcggt ctggcttgtt gggtgggcag gtcagctcgt cggcgggcag attgttgatc
      541 ttcgcggcgg cgacgcgcgg gtccatatcg gcgatctcgc cgtacccgga gtgaatgaga
      601 tagccgggat agacggtggc aaagcgcgag aggatcgccg cggccttttc accataggcg
      661 cgctcgcccg tgagcgcgta gaccatggcg aggtcgcgcg cggcgcgcgc catgtagccc
      721 gtcttgtgcg cccggatcgc cccggtccac gacgagcgga gatgaatgtt gttgaaattc
      781 caggacttgc cgccgtagaa ggtgaagcgt tgctcgccgc cgtggcgggc gacgaggacc
      841 aggtcctcgg gatacttctc gttgggatag acggtgccgc agttgcggca tttgagctcg
      901 tcgggccgtt tgacatccca gatatattgg ccgtggaccg gcgcgtactc gcagtggggg
      961 cacatcgtga agagcgtgca gagcggcgtt agcgccggga tcatctgctc gacgaactcc
     1021 gggggctgat tgaggacgta atccgcgcgc ccgcggtagc ctgcgagaag ccgcttcgcc
     1081 caggggtgcc ggcggacgtt ctctctcgcg cgctccaggt cggccggctt gatgaaggcg
     1141 atgggccggc gcacgctcgc gcggtacttc tgccacagcg acaggtcggc ggggtcaaag
     1201 tgctcgcccc ccaggtcata gacttgcctg aagcgcgcgg gcactgtgat gcccggcgct
     1261 ttcgagagcg cggcggcgag ggccggcgcc atctgctggc cggcaaacgc ggccaggtga
     1321 aaggtgacct tcacctccgt ctgaggagcc acctttcctc ccaggggcgc cgagtaggag
     1381 acggccgggt actcgcggtt ccggcccagt tgcggctgcc cgtcggggag gcagaaggcg
     1441 atcccctcgc ctttctctgg gtcgtacgcg gccacccagg gcggggtgcc gggatcccgg
     1501 cgcacggcat cgcccggccc caggagcagg ctcccgccgg agctgggcac cgcccctcgc
     1561 gacagccgcg gagcgaacca gatcaggggc aggctcatgc cgccgcgcaa cgagatctcc
     1621 cccacggcct ctaggcggta gtcgttttcg agaacgggcg agccggcgcg cagcgtgtgc
     1681 gtcttcacga agcggaagtg cttcgcctcg ccctcagcgg ggtagctcac caccagacgg
     1741 acgaaatcat ccccccgctc cacgcgcgcc gtcacaggtc tcagcccgta ctcgggctcc
     1801 gtcttgttat cctgatacca gtactgcgcc ccctccacgg agtagctgag aatgcagatg
     1861 ttgatcaggt tctcgccgtc aggcacgcgc cggaaatcgg cgaggacgta gttggctttc
     1921 gcggggaaaa ccgcgcgcac gatgccgttt tcgagggtca ctccctccgg cgtctctacc
     1981 gccgaaggcg cggccaaagc cggaagagca aagagaagtg ctacaagcac gagccaggtt
     2041 actcgcatgg ggctacctcc cgccgccagt cgtcatggca gcttgcgata ctattctgga
     2101 accggctctc ccctcctgct ggtggtctta gcacgagcgc tgcgagacgc gcgcaacccc
     2161 acgcctgcag cgcggcg
//
LOCUS       DAOSCV010000799         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33130_length_2176_cov_3.682697, whole genome shotgun sequence.
ACCESSION   DAOSCV010000799 DAOSCV010000000
VERSION     DAOSCV010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33130_length_2176_cov_3.682697"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..1426
                     /gene="ispG"
                     /locus_tag="PLY56_16380"
     CDS             218..1426
                     /gene="ispG"
                     /locus_tag="PLY56_16380"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOJ23106.1"
                     /translation="MSSVDRNTSIATALPRRRTRPVRVGNVIIGGDHPVVVQSMINVP
                     TRNVAACVDAIERLYRAGCEIVRVTAPTMEDARAIGEIRALLKRRGLSVPLAADVHHD
                     GTRIAVEVARHVDKVRINPGLFVYRKRRAGIEYTEEETREHLEAIERDLLPVLEACRE
                     SGAALRIGVNHGSLSDRILMTYGDTPEGMVESALEFVRLCEKHDFRELVISLKASRVA
                     VMIQANRLLARRMDALGMDYPIHLGVTEAGDGDYARIKSTAGIATLLAEGIGDTIRVS
                     LSEDPLEEIPVCYDILQALGLRRTKTEFIACPSCGRTRFDLPTVLAQVKAATGHLAGL
                     NIAVMGCIVNGPGEMADADYGYVGRGRGVIALYRGHELVKTDVPEQDGVRELVALIKA
                     DGRWVDPPAS"
     gene            1562..1984
                     /locus_tag="PLY56_16385"
     CDS             1562..1984
                     /locus_tag="PLY56_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23107.1"
                     /translation="MAAEQEHDVAAANVAHKMRVPIWEVHANVGDAPRRAVPVPGGDD
                     VTPDIVAFEKHTGRLAAIAAVETASTLSAGASEWKLFQLLAPKFFLYVPEECLEEATR
                     LCRRHGIPLASLRVYYYNSRNQFITERGWRAQKRKAAS"
BASE COUNT          363 a          733 c          737 g          343 t
ORIGIN      
        1 cgcgcgccgg gttgcaccct gcctcccagg cttcggatcc cttatgatcc cttatgatcg
       61 gaatctcagt atagctgtgg cgcggtttga cccaccgtga tccggtgtgc tataatcacc
      121 gtgcgaggca ggccgcgcgc cggcgcccat cctccccatc gcctgccggt gcgcgcatgg
      181 agtatctccg ccctcgaaac cccagcggga gcaagagatg agcagcgtgg acagaaacac
      241 cagcatcgcc acggcgctgc cgcggcggcg gacgcgccca gtgcgcgttg ggaacgtgat
      301 catcggcggc gaccatccgg tcgtcgtcca gtcgatgatc aacgtgccga cgcggaatgt
      361 agccgcgtgc gtggatgcga tcgagcgcct gtaccgcgcc ggctgcgaga tcgtgcgcgt
      421 gacagcgccc accatggaag acgcgcgcgc catcggagag atccgcgctc tcctgaagcg
      481 ccgcggccta tccgtgcccc tggccgccga tgtccaccac gacggcacgc gcatcgccgt
      541 cgaagtcgcc cggcatgtcg ataaggtgcg catcaacccg gggctgttcg tctaccgcaa
      601 gcgccgggca ggcattgaat acaccgagga ggagacccgg gagcacctgg aggccatcga
      661 gcgcgacctt cttcccgtgc tggaagcctg ccgggaatcg ggcgcggcgc tgcgcatcgg
      721 cgtcaatcac ggctcgcttt ccgatcgcat cctcatgacc tacggcgaca cgcccgaggg
      781 gatggtggaa tcggcgctag agttcgtgcg cctctgcgag aagcacgatt tccgcgaact
      841 cgtcatctcg ctgaaggcct ctcgggtggc ggtgatgatc caggccaacc ggctcctcgc
      901 gaggcgcatg gatgccctgg gcatggatta cccgattcac cttggcgtca ccgaggcggg
      961 cgacggcgac tatgcgcgca tcaaatccac cgcagggatc gccacgctcc tggccgaggg
     1021 gattggagac acgattcgcg tctcgctctc cgaggatccc ctcgaggaga tcccggtctg
     1081 ctacgacatc ctccaggcgc tgggcctcag gcgcaccaag acagagttca tcgcctgccc
     1141 atcctgcggg cgcacgcggt tcgacctccc caccgtcctc gcccaggtga aggcggcaac
     1201 agggcacctc gccgggctca acatcgccgt catggggtgc attgtcaacg ggccgggcga
     1261 gatggctgac gccgactacg gctacgtcgg ccgcggccgc ggcgtcattg ccctctaccg
     1321 ggggcacgag ctcgtcaaga cggacgttcc cgaacaagac ggcgtgcgtg agctggttgc
     1381 gttgatcaag gcagatgggc gctgggtcga cccgccggca tcgtgaaccg ccggaggccc
     1441 ggcgttcgcg gccagccagg agagatcgcc ccgggcgagc ttcctcccat gggcaggcat
     1501 cgaggccgtt gatctcacga gccggccggg cattggggaa gccgagagga gttaaccggg
     1561 agtggcagcg gagcaagagc acgacgtcgc agcggcaaac gtggcgcata agatgcgcgt
     1621 gccgatttgg gaggtccacg ccaacgtggg cgacgctccc cggcgagcgg tgcccgtgcc
     1681 aggcggcgac gacgtgacgc cggacatcgt cgcgttcgag aagcacaccg ggcgcctggc
     1741 ggccatcgct gcggtggaga cggcttcgac gctgagcgcg ggcgcgtcgg agtggaagct
     1801 gttccagctg ctagcgccga agttctttct ctacgtgccc gaggaatgtc tggaggaggc
     1861 gacgcgcctc tgtcgccggc acgggattcc cctcgcctcg ctgcgggttt actactacaa
     1921 ctcgcggaac cagttcatca ccgagcgggg ctggcgcgcc caaaagcgaa aggcggcttc
     1981 ctaagcgcgc cccgggctgg gaaggaaggc cgatgcgttt tcaggcagaa cgcgcgcatg
     2041 cggacgcggt agcgatcgcc ttcccgcgcc gctctggctc cgagggctcg gcgcgcgccc
     2101 gcgggcacat cgtagcgcag ctgcgcgccg ccggcctgga tccgatcctc gagccgttca
     2161 ccttttcgcc ggtcct
//
LOCUS       DAOSCV010000800         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33179_length_2173_cov_3.200661, whole genome shotgun sequence.
ACCESSION   DAOSCV010000800 DAOSCV010000000
VERSION     DAOSCV010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33179_length_2173_cov_3.200661"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLY56_16390"
     CDS             <1..476
                     /locus_tag="PLY56_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23108.1"
                     /translation="VEWVIGQIDIRDTRPVFGAPCEGQGLSHFKYRNGVRGLMITGYE
                     ADLGASNRLIGTDGVIEVGVAGGPDLRVWTKGSAGWETVPVEGGLHGSDGFRLGVLDL
                     LDALKTGREPELSGRRAFQDTELIFATYESSRRRGRVDLPLEIEDSPLQEMLDQA"
     gene            691..1848
                     /locus_tag="PLY56_16395"
     CDS             691..1848
                     /locus_tag="PLY56_16395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HOJ23109.1"
                     /translation="MKAVVYHKPKEIRVEDVDDPKIQDPRDAIVRVTSTAICGSDLHI
                     YNGTFPQARPMVLGHEFMGVVEEVGKEVSTLKKGDRVVVPFPIACGTCWFCHHDLPIH
                     CENSNPKHYGPEGGVLKEKGGGLFGYTDLYGGYDGGQAEAVRVPYADYGPRKVPEGVP
                     DEQVLFLTDIWPTGWAALDWAQLRGGETVAVFGCGPVGLMAQKAAWIQGAGRVIGIDI
                     QEYRLEMARKAANAETVKGKGDHVVQAIRDMTDGRGADVCVDAVGMEADIGALRKAAA
                     AIHLEAGTIDALQTAIRAVRRGGAVPVVGVYGTSYDNFPWGQIFDKGLRFHAGQAPVH
                     YYIDRLMQLILEGKVTTHDIITHTLPLSEAPNAYRIFNEKEDGCVKVVLKP"
     gene            complement(1936..>2173)
                     /locus_tag="PLY56_16400"
     CDS             complement(1936..>2173)
                     /locus_tag="PLY56_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOJ23110.1"
                     /translation="AMETLLPEGRILALQRGANVVMLNVTEMGYRQSYALYPGKAGAT
                     DTPAESRARLLAKLQSIGRRPGSGYGSHQRRREA"
BASE COUNT          383 a          685 c          753 g          352 t
ORIGIN      
        1 cggtcgaatg ggtcatcggg cagatcgaca tccgggatac ccggcccgtc tttggtgcgc
       61 cgtgcgaggg ccaggggctg agccacttca aataccgcaa cggcgtgcgc gggctgatga
      121 tcaccggcta tgaggccgac ctgggcgcga gcaaccggct gatcggcacc gatggcgtca
      181 tcgaagtcgg ggtagcgggc gggcccgacc tgcgcgtgtg gacgaagggc agcgccggct
      241 gggagacggt tcccgtcgag ggcggcctgc acggctccga tgggttccgc ctgggggtgc
      301 tggacctgct tgatgccctg aagacgggcc gcgagccgga gctatccggg cggcgcgcgt
      361 tccaagacac ggagctgatc tttgccacct acgagtcgag ccgccggcgc gggcgcgtcg
      421 atctcccgct ggagatcgag gattcgccgc tgcaggagat gctcgaccaa gcctgactcc
      481 aagatgcgag gagcggcgga tgcacccgcc cccgctcctc gcatcgcacc acaccccacc
      541 cacgcgcagt aggtacgccc gaggcatcaa cgcacccgcc ccaagcaggc cgcaccccgt
      601 ctccgcgctc cccacccctc tccggacccg gtttggcccg ccgccgaggg ggaaacctcg
      661 cctgaggata tcccatggga ggagatgggg atgaaggcgg ttgtctacca caagccgaag
      721 gagatccgcg ttgaggacgt agatgatccc aagatccagg acccgagaga cgccattgtg
      781 cgcgtcacct cgacggccat ctgcggctcg gatctgcaca tctataacgg gaccttcccc
      841 caggcgaggc cgatggtgct cggccacgag ttcatggggg ttgttgagga ggtcgggaag
      901 gaggtcagta ccctgaagaa gggcgatcgt gtggtggtgc cctttcccat tgcgtgcggc
      961 acgtgctggt tttgccatca tgatctcccg atccactgcg agaactctaa tccgaagcac
     1021 tacggcccag agggtggcgt gctgaaggag aagggcggcg gcctcttcgg ctatacagac
     1081 ctctacggag gctatgatgg cggccaggcg gaggcagtgc gcgtgcctta cgccgactat
     1141 ggtccccgga aagtgcccga gggcgttccc gacgagcagg ttctgttcct cactgatatc
     1201 tggcccacgg gatgggcagc cctggattgg gcacagcttc gcgggggaga gaccgtcgcg
     1261 gtgttcggat gcggaccggt gggccttatg gcgcagaagg ccgcttggat tcagggcgcc
     1321 gggcgggtca tcgggatcga catccaggag taccgtctgg agatggcacg caaggcggcc
     1381 aacgcggaga cggtgaaggg aaaaggcgac cacgtggtgc aggcgatccg cgatatgacc
     1441 gatggccgcg gcgccgatgt ctgcgtggat gccgtcggca tggaggcgga tatcggggcg
     1501 cttcggaagg cagcagctgc aatccacctc gaggccggga ccatcgacgc gctgcagacc
     1561 gcgatccgcg cggtgcgccg gggtggcgcc gttcccgtcg taggcgtcta cggcaccagt
     1621 tacgacaact ttccctgggg tcagatcttc gacaaggggc ttcgcttcca tgccggacag
     1681 gcccccgtac actactacat cgaccgcctg atgcaactca tcctggaggg aaaggtgact
     1741 acgcacgaca tcatcacaca caccctgccc ctctccgagg cgcccaatgc ctaccgcatc
     1801 ttcaacgaga aggaggatgg gtgcgtgaaa gtggtgctca agccctgaga tgtggagcga
     1861 cggggccgcg gatcgaggcg atgcgcccgg cttgaagctc gcggcagacg cactgccggc
     1921 gggcagcctg gcgcgttacg cctcgcgccg ccgctggtgc gatccgtagc cactccctgg
     1981 acggcgcccg atgctttgga gcttcgcgag aagccgggcg cggctctcgg cgggggtatc
     2041 ggtagcgccc gccttacccg gatagagcgc gtaactctgc ctgtagccca tctcggtgac
     2101 gttcaacatc acgacgttcg cgccgcgctg gagagcgagg atccggcctt ctgggagcag
     2161 cgtctccatc gcg
//
LOCUS       DAOSCV010000801         2172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33204_length_2172_cov_2.554558, whole genome shotgun sequence.
ACCESSION   DAOSCV010000801 DAOSCV010000000
VERSION     DAOSCV010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33204_length_2172_cov_2.554558"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PLY56_16405"
     CDS             <1..1024
                     /locus_tag="PLY56_16405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ23111.1"
                     /translation="EPLERGFGTTIGNSLRRVLLSSIQGAAITTVKIDGVQHEFSTIP
                     GVAEDATDIILNLKNLAIKITSDGKQESGTGEEAEPEPIRLVIDREGEGEVTAADIET
                     PPEVEIVNRGAHIATLAPDGRLRMEMIVERGKGYVPAEKHARREEVIGEISVGSIFTP
                     VPKVTYSVEPTRVGHKTDFDRLTLEIWTNGTIDPRSALSQAARVLDSYLHLFFEVGEE
                     SETVLLGPGTAPSRAGKQRALNTRIEDLDFSVRTSNCLRNQGIETLEDLLQRTEEELL
                     ATRHFGKKSLSEVKEKLEQYGLKLASGKEGAETARASRQEAEEVEAEPAEESPVAATE
                     EAPAEA"
     gene            1054..1422
                     /gene="rplQ"
                     /locus_tag="PLY56_16410"
     CDS             1054..1422
                     /gene="rplQ"
                     /locus_tag="PLY56_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ23112.1"
                     /translation="MRHRVSGRKLGLPSDQRMALLKNLVQAVIEHEAIETTAGRGRET
                     QRMVERIISTAREDTLHNRRQTRRLLGGNARGESLVTKLFTEIAPRYADRSGGYTRLV
                     KIGPRRGDAAERVRLELVEG"
     gene            1499..>2172
                     /gene="truA"
                     /locus_tag="PLY56_16415"
     CDS             1499..>2172
                     /gene="truA"
                     /locus_tag="PLY56_16415"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOJ23113.1"
                     /translation="MICPDRFLATLPRSDERDAEGRRNIAVLLQYDGTEFLGFQRQAQ
                     IPTVQQTLEEGLERLLKHPVRITAAGRTDTGVHAAGQVINFRTTARIPEDRICPALNS
                     VVPKSIVAYAAAQVAPRFSARFSARSRVYYYVMATGDYPSVFTRRFTYHYPWPLDVAA
                     MNRAASYFRGNRDFRSFCVEAGPQENTIRTVFLVRCRQRRGLLITVVHANAFLRGRVR
                     AVMGTL"
BASE COUNT          412 a          678 c          743 g          339 t
ORIGIN      
        1 cgagcccctg gagcgggggt tcggtaccac gattggcaac tcgctgcggc gggtgctcct
       61 ctcctccatt cagggggcgg cgatcaccac cgtcaagatc gatggtgtcc agcacgagtt
      121 ctccaccatc ccgggtgtgg ctgaggatgc taccgatatc atcctgaact tgaagaacct
      181 tgctatcaag atcaccagtg atgggaagca ggagtccggt accggggagg aggccgagcc
      241 ggagccgatc cgccttgtga tcgaccgcga gggtgagggc gaggtaaccg ccgcggatat
      301 cgaaaccccc ccggaggtgg agattgtcaa ccggggagcg cacatcgcca ccctcgctcc
      361 ggatggccgc ctgcgcatgg agatgatcgt cgaacgcggc aaggggtatg tgcccgcgga
      421 gaaacatgct cgccgcgaag aggtgattgg cgagatctct gtggggtcga tcttcacccc
      481 agtccccaag gtgacctatt ccgtcgagcc gacgcgcgtc ggccataaaa ccgacttcga
      541 ccggctgacc ctggagattt ggacgaacgg gacgattgac ccgaggtctg ccctctcgca
      601 agccgcgcgc gttctggata gctacctgca cctcttcttc gaggttgggg aagagagcga
      661 gaccgtgctt ctgggcccgg ggacggcgcc gagccgcgcg ggcaagcagc gcgcgctgaa
      721 cacccggatt gaagaccttg atttctcggt gcgtacctcc aactgcctgc gcaaccaggg
      781 gatcgaaacc ctggaggacc tgttgcagcg cacggaggaa gagctgttgg ccacgcgcca
      841 cttcgggaag aagtcgctca gcgaggtcaa ggagaagctg gagcagtatg ggctgaagct
      901 tgccagcggc aaggaaggcg ccgagacggc gcgcgcaagc cgccaggaag ccgaggaggt
      961 tgaagccgaa cccgcagagg aaagcccggt agctgcaacc gaggaagcgc cagcggaggc
     1021 gtgaggcccg ctggctggta ggaggaggaa cagatgcgac atcgtgtctc ggggcgtaaa
     1081 ctggggcttc caagcgacca gcgaatggcc ctgctaaaga acctcgtgca ggcagtcatc
     1141 gagcacgagg cgatcgagac gacggctggc cgtggccgtg aaacccaacg catggtggag
     1201 cggatcatct ccaccgcgcg agaagatact ctgcacaacc gccgccagac gaggcggctc
     1261 ctgggcggaa atgcccgcgg cgagagcctg gtgaccaagc tgttcaccga gatcgccccg
     1321 cgctacgcgg atcgctccgg tggctacact cgcctcgtga agatcggccc gcgccgcggc
     1381 gatgccgccg agcgcgtgcg cctcgagctg gtcgagggct aagcgaacgg ccggcgcgga
     1441 gaggcgcggt gccgcctgac gcccggatcg caccttgata gagtgacgag gagacgccgt
     1501 gatctgcccg gaccggttcc tcgccacgct gccgcgcagc gatgagcgcg acgccgaggg
     1561 acgccgtaac atcgccgtcc tcctgcaata cgacggcacg gagttcctcg gctttcagcg
     1621 gcaggcgcag atccccaccg tgcagcagac gctggaggag ggactggagc gcctcctcaa
     1681 gcacccggtg cggatcaccg ccgcaggacg aaccgacacc ggggtgcacg cggccgggca
     1741 ggtgatcaac tttcgaacaa cggcgcgcat cccagaggac cggatctgcc ccgcgctgaa
     1801 cagtgtggtg cccaagagca tcgtggccta cgccgcggcc caggtggccc cccggttcag
     1861 cgcgcgcttt tccgcgcgaa gccgggtgta ctactacgtg atggcgaccg gcgattaccc
     1921 ctcggtcttc acacggcgct tcacgtatca ctatccgtgg ccgctggacg tcgcggcgat
     1981 gaaccgggcc gcgagctact ttcgggggaa ccgcgacttt cgttcttttt gtgttgaggc
     2041 gggcccgcag gagaacacca ttcgcaccgt ctttttggtg cgctgccgtc agaggcgcgg
     2101 gctgctgatc acggtggtgc atgccaacgc cttccttcgg ggcagggtgc gcgccgtgat
     2161 gggtaccttg at
//
LOCUS       DAOSCV010000802         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33296_length_2167_cov_3.403883, whole genome shotgun sequence.
ACCESSION   DAOSCV010000802 DAOSCV010000000
VERSION     DAOSCV010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33296_length_2167_cov_3.403883"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLY56_16420"
     CDS             <1..532
                     /locus_tag="PLY56_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ23114.1"
                     /translation="IDHYARVASAGLPLIAYHIPALTGQRMTPELMARLAEIPGVAGL
                     KFTDNDFFLMRNLIEDGAGRWIVFNGSDEIFLCGLAMGAHGGIGSTYNVMPAAYVSLF
                     RAFQAGDLRQAQELQFRITRAVRAIVAHPTIGALKAMMSARGFDVGEPRRPLPPVSAA
                     VGRQLCETLDQIFATG"
     gene            complement(560..1063)
                     /locus_tag="PLY56_16425"
     CDS             complement(560..1063)
                     /locus_tag="PLY56_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23115.1"
                     /translation="MTNAGEQAAGATSERVGWGWRVLTLAWTGLAVAVALRLRRESLK
                     PEVIRRDEGALRRACLGFPLPVMLGGSQVKDSRDQDTGTRTLSYRLPATQNQDEILAY
                     YDDYFRKSGFVPTDGEPDALPGGVERAYHDACRLRAVTLSVRREPPEGDHHAVILTVQ
                     PIPQSDQ"
     gene            1767..>2167
                     /locus_tag="PLY56_16430"
     CDS             1767..>2167
                     /locus_tag="PLY56_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23116.1"
                     /translation="MIAEAASADGLASQLAQSTWLLEWQRQRSDAGVSRSFGEWQPMA
                     AVRSQGTPAGLLPLTLQLGSSSRLNLAYGLAAADQNSRDPIRVGVQGLGFSTNFGAVK
                     SESQLYFSRPSLDGGAAATLVGDLGRSTAREG"
BASE COUNT          357 a          755 c          679 g          376 t
ORIGIN      
        1 aatcgaccac tatgcgcgtg tcgcctccgc cggccttccg cttatcgcgt accacatccc
       61 ggcgctcacc gggcaacgga tgaccccgga gctgatggcc cggctggcgg agatcccggg
      121 cgtggcgggt ctgaagttca cagacaacga cttcttcttg atgcgcaacc tgatcgaaga
      181 cggcgccgga cgctggatcg tcttcaacgg ctcggacgag atcttcctct gcggcctcgc
      241 catgggcgct cacggcggca tcggctccac ctacaacgtg atgccggcgg catacgtcag
      301 cctcttccgc gccttccagg caggcgactt gcggcaggcg caagagctgc agttccgcat
      361 cacgcgggcc gtccgcgcta tcgtcgccca tcccacgatt ggcgctttga aggcgatgat
      421 gagcgcgcgc gggtttgatg tgggcgagcc acggcgcccg ctgccccctg tctccgcggc
      481 agtcggacgc cagctttgcg agacgctcga tcagatcttt gccacggggt gatcggcgtg
      541 gccaggcagg cagggcgcct tactggtcac tctgcggaat cggttgcact gtgaggatca
      601 ccgcgtggtg atcgccctcc gggggctcac gccgtacgct gagagtgaca gcgcgcagcc
      661 ggcatgcgtc gtggtaggcg cgctccaccc cacctggcag cgcgtccggt tcgccgtcgg
      721 tcggcacaaa cccgcttttc cggaagtaat cgtcgtaata cgcgaggatc tcgtcctggt
      781 tctgggtggc aggcagcctg taagagagag tgcgcgtccc ggtgtcttga tcgcggctgt
      841 ccttcacctg cgagccacca agcatcacgg ggagcgggaa gcccaggcag gcacggcgaa
      901 gggccccctc atcgcggcgg atcacctccg gcttcaggct ctcgcggcgc aggcgtagcg
      961 ccacagccac tgccaggccc gtccacgcga gggtgagcac gcgccagccc cagcccacac
     1021 gctccgaagt ggctccggcc gcctgctctc cagcatttgt catcgcgtct ctcctcgcag
     1081 ccagcgcggt cacggacggg ggaaggccgc gcctatcaga cacgcgttcg ccccgtcgcg
     1141 cgtcgccttg gcactccccc tccagaaatg gcgcctatcc ctctcgctcg ttcccccatg
     1201 tttcccctgc gcgacccacc atccggtaca tcgagacgcc ctgccgccgg gagaaaatct
     1261 ctaagcagag aatacccctt caggcccgct ctctcctgcc ctctcctctc ccacacgcct
     1321 ccggcagatc ggccggtccg gtggccgtcc tatgtagact catatacaca agatacacgc
     1381 gcggatactg gaaacaaacg ggcgtgggaa gcatctaaca gacgtggctt gctcaagcgg
     1441 gccgcatcct gatgaagcgc gtacggaacc tcctgggtgt gccgtttttt ctgcgtgttc
     1501 gttcgacagg cgcgcccagt gagcggatag accgggcgaa ccggccggta caggaacggt
     1561 ggagaagcgc ctcgccctcc gcttcgggaa tcggagcacg ggcagaagac gcgtggctcc
     1621 gactgtccct cgccaaaaaa cggccgaatc ggtcggtacc cttggttacg cctcctatca
     1681 ggcaaactcg tcgcatctat cccatcgcca gacctctcgc catcgtcctc ctcgccctgc
     1741 tcagcatcct ggcaagcccc gtgacaatga tcgcggaggc cgcgagtgcc gacggtctgg
     1801 ccagccaact ggcacagagc acgtggctgc tggagtggca gcggcagcgc agtgatgccg
     1861 gggtgtcgcg ctccttcggc gagtggcagc ccatggcggc ggtccgaagc cagggaacac
     1921 ctgcaggctt gctgccactt accctgcagc tgggcagctc gtcgcggctc aacctcgcct
     1981 acgggctcgc cgcggcggac cagaattcgc gcgatccgat tcgggtcggg gttcagggtt
     2041 tgggcttctc gacgaacttc ggggcggtga aaagcgaatc gcagctctac ttctcgcgcc
     2101 ccagcctcga tggaggcgca gccgccaccc tggtgggcga tctgggccgc agcaccgccc
     2161 gagaggg
//
LOCUS       DAOSCV010000803         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33330_length_2166_cov_3.553292, whole genome shotgun sequence.
ACCESSION   DAOSCV010000803 DAOSCV010000000
VERSION     DAOSCV010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33330_length_2166_cov_3.553292"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1301
                     /locus_tag="PLY56_16435"
     CDS             222..1301
                     /locus_tag="PLY56_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOJ23117.1"
                     /translation="MAGFNGLGMHLGNLARLSSARSRSISPENFTGEKGRGGMATEGT
                     GADKARDLGPGWKISPSVRISPGETFTLADIREPGAIQHIWMTPTGPWRFSILRIYWD
                     DCEVPSVECPVGDFFACGWGKFAQVSSLAVCVNPGSGFNCYWEMPFRKRCRMTMTNLG
                     DEAMTLYYQIDYTLTEVPDDAAYFHAQFRRVNPLPYKQVYTILDGVKGRGHYVGTYMA
                     WGVNNTGWWGEGEIKFYLDGDEYPTICGTGTEDYFCGSYNFENRETRQYQEFTTPYSG
                     LPQVIRPDGLYQSQQRFGLYRWHIMDPIRFEQDLRVTIQALGWRSGGRYLPLQDDIAS
                     VAYWYQTLPPAPFPPLPDRDYLEII"
     gene            1527..>2166
                     /locus_tag="PLY56_16440"
     CDS             1527..>2166
                     /locus_tag="PLY56_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23118.1"
                     /translation="MRYLRWCFSLLLVVAGVPASAFAEGAPAGLIQRLPEATIEWGEA
                     RVFLFGEGQPREGVEGLRKQLSARNAARRHALTACEKLAASFLPGDAQLTDEQRSRLR
                     DLLRSSRLHTKEAPETRLAGYAELGWNAAKERFRVVVEVPLFGARSIAAALSPLPMPP
                     AGTQTPEGEAAGETRSAGQVATGLIINCTRTQLKPSLHPALLRKGDAKPFYGP"
BASE COUNT          360 a          707 c          749 g          350 t
ORIGIN      
        1 gggggaggag cgcgcccggg acccgcgaaa agggggagaa acaggcgcct gtaggccggc
       61 cgtgtgagcc aaacgccccg gccggagggc gcatcgcaaa gcgccagagg ggcgatgact
      121 gggcattgag gatcgcggcg gctgttgccg ggccccatcc ccgggcgccc tggaacctgg
      181 cggacgggcg ttcactggcc catctcacgg aggagcatac catggcaggc ttcaatggac
      241 tgggaatgca cttgggcaac cttgcgcgcc tctccagcgc caggtcgcgc tcgatcagcc
      301 cggagaactt cacgggcgag aaggggaggg gcggcatggc gacggaggga accggcgccg
      361 acaaggctcg cgacctgggt ccgggctgga agatctcgcc ctcggtgcga atcagcccgg
      421 gagagacctt cacgctggcg gatatccgcg agccgggcgc catccagcat atctggatga
      481 caccgacggg gccctggcgc ttcagcatcc tccggatcta ctgggacgac tgcgaggtcc
      541 cgtcggtcga gtgccccgtg ggcgatttct tcgcgtgcgg atgggggaag ttcgcccagg
      601 tctcctcgct cgccgtgtgc gtcaatcccg gcagtggctt caactgctac tgggagatgc
      661 cgttccgcaa gcgctgccgg atgacgatga ccaacctcgg tgacgaggcg atgaccctct
      721 actaccagat cgactacacg ctcaccgagg tgcccgacga cgccgcctac ttccacgcgc
      781 agttccggcg cgtcaacccg cttccctata agcaggtcta caccattctg gacggagtga
      841 agggccgagg ccactatgtg ggcacctaca tggcgtgggg ggtgaacaac accggctggt
      901 ggggcgaggg cgagatcaag ttctacctcg atggcgatga gtacccgacg atctgcggca
      961 ccggcaccga ggactacttc tgcggctcct acaacttcga gaaccgcgag acgcgccagt
     1021 atcaggagtt caccacgccc tattccggac tgccccaggt gatccgtccg gacggcctct
     1081 accagtcgca gcagcggttt ggtctttacc gctggcacat catggatccg attcgcttcg
     1141 agcaggatct gcgggtgacg atccaggcgc tcggatggcg tagcggcggg cgctaccttc
     1201 ccctgcaaga cgacatcgcg tccgtcgcct actggtatca gacgctgccg cccgcgccct
     1261 tcccgccgct gccggaccgg gactatctgg agatcatctg aggggctctc cgcgcgccct
     1321 gcacccaagc ggaagcagtt cctcttcgcc gcctcctctc gggcggcgaa gaggcgcccg
     1381 gcccggaccc ggccgatcct cgcttcttgg aacttcccgg ggagtgacag gcgtcctaag
     1441 agggagagga gttggggtgc aggccccgaa gccggtactg gaggcgtgat ccgggccggt
     1501 cgagtgccag gaagggggat cgggcgatgc gatacctacg ttggtgcttc tcgctgctgt
     1561 tggttgtggc cggcgtgccc gccagtgcgt tcgcggaggg cgctcctgcc gggctgatcc
     1621 agcgattgcc cgaggccacg attgaatggg gcgaggcgcg agtcttcctg tttggcgagg
     1681 gccagccgcg agagggcgtt gagggcctgc ggaagcagct gtcagcgcgg aacgcggcgc
     1741 gcaggcatgc cctcaccgct tgcgagaagc tcgccgcgtc gttcctgccg ggcgatgccc
     1801 agctgacgga tgagcagcgc tcccgcctga gagacctcct tcgaagcagc cggctgcata
     1861 cgaaggaggc tcccgagacc cggctggccg gctatgcgga gctcggctgg aacgccgcca
     1921 aggagcgctt ccgggtggtg gtcgaggtgc cgctctttgg cgcgcgcagc atcgccgccg
     1981 cgttgtcgcc cctccccatg ccgccggcag gcacccaaac tcccgagggc gaggcggctg
     2041 gtgagacgcg ctccgcgggc caggtagcta ccggtctgat catcaactgt actcgcaccc
     2101 agttgaagcc ctccctgcat ccggcgctgc ttcggaaagg cgacgcgaag ccattctacg
     2161 ggccgg
//
LOCUS       DAOSCV010000804         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33364_length_2165_cov_2.312796, whole genome shotgun sequence.
ACCESSION   DAOSCV010000804 DAOSCV010000000
VERSION     DAOSCV010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33364_length_2165_cov_2.312796"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLY56_16445"
     CDS             complement(<1..181)
                     /locus_tag="PLY56_16445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23119.1"
                     /translation="MASDLGTGILGVAEARAGAPIAVSEGPGTSGSAREHPADRPDQD
                     LGQERLLDEAGDSICL"
     gene            368..1309
                     /locus_tag="PLY56_16450"
     CDS             368..1309
                     /locus_tag="PLY56_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ23120.1"
                     /translation="MAESTQQFRHASEKLKRLASQGWEWGRHIYQEFTGEHASLTAAA
                     IALFGILSIFPLALLAISMAAYALGSQEQALRQIEQVAGQLLVGDASRTLTSVLRGVV
                     ESRGIAGFVGLIGFLWAASRVFTIMAEAFNMAWDVPESRGFFKRNLLAIGLVLLSLIF
                     GMLMFVGPLAVSFLLRYGDRVAAQVGAPTGISAFWPALVTVLAFIATIAFFFILYKYV
                     PNRPVPWQSALAGAITAAVLWEIARYLFQLYVVNFASYNEVYGALAGAIILILWLYYS
                     AMILLLGAVTSAVHDARVFHDRAEHPQRRGYIEIAEP"
     gene            1390..1475
                     /locus_tag="PLY56_16455"
     tRNA            1390..1475
                     /locus_tag="PLY56_16455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1423..1425,aa:Leu,seq:cag)
     gene            1614..1760
                     /locus_tag="PLY56_16460"
     CDS             1614..1760
                     /locus_tag="PLY56_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23121.1"
                     /translation="MGHTLVPGAQPATRISRSPVAGSDAAKHPQAVSPGMQGERMALK
                     PYSL"
     gene            complement(1748..>2165)
                     /locus_tag="PLY56_16465"
     CDS             complement(1748..>2165)
                     /locus_tag="PLY56_16465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23122.1"
                     /translation="VGDYLGHRSYGYHIDLMPEKKPENVCGVSLSEIQRPAEIAMVAD
                     VLQDPNVRGRLHTPSRGKMVTDPDGSNCSICGGKHLSMFPSSDKYHDYQRPGFNVLER
                     HNGVANVGFADGHVKAMKHVTLYNNGSKAPYFNYSE"
BASE COUNT          365 a          718 c          655 g          427 t
ORIGIN      
        1 ggagacagat ggaatcgccg gcctcatcca gaagccgttc ctgcccaagg tcctgatccg
       61 gacgatccgc cgggtgctcg cgggctgacc cgctggtgcc aggtccctcc gacaccgcga
      121 tgggtgcccc cgcgcgcgcc tctgcaaccc cgaggatccc ggtgcccaga tccgacgcca
      181 tgtatcacgg cctgcggccg cgaagcggtc accgccgcat ttcgatctcg cctggtttgt
      241 ggctgttgcc ccctgggtaa cgccattctc aggtgagaag ggatgaccag acccgattcc
      301 cggccaggcc atgggcacca gggggagcgc cggcggtcac ccaaagcgga gaggcaggaa
      361 ggcggcgatg gcagagagca ctcaacagtt ccgccatgcg tccgagaagc tgaagcggct
      421 cgcctcgcag gggtgggaat ggggccgcca catctaccag gagttcaccg gggagcatgc
      481 atcgctcacc gcggctgcga ttgccctctt cggcatcctc tctatcttcc ctctggcgct
      541 cctggcgatt agcatggcgg cctatgccct ggggagccag gagcaggcgc tccgccagat
      601 tgagcaggtc gccgggcagc tcctcgttgg cgatgccagc cggacgctga ccagcgtcct
      661 ccgaggcgtc gtggaatccc ggggaatcgc cggtttcgtc ggtctgattg gcttcctttg
      721 ggcagccagc cgcgtcttca cgattatggc cgaagcgttc aacatggcct gggacgttcc
      781 ggagagccgg ggtttcttca agcgcaacct cctggccatc ggccttgtgc tcctctcact
      841 catctttggc atgctgatgt ttgtcgggcc gcttgccgtc agttttctgt tgcgctacgg
      901 ggatcgagtt gcggcgcagg tgggggctcc gaccggaatc tctgccttct ggccggcgct
      961 cgtcaccgtg ctcgctttca tcgccaccat cgccttcttc ttcatcctct ataagtatgt
     1021 gccgaaccgg cccgttccct ggcagtccgc actcgccggc gcgatcacgg cggcggtcct
     1081 ctgggagatc gctcgctacc tcttccaact gtacgtggta aacttcgcct cctataacga
     1141 agtgtatggc gcgctggcgg gcgcgatcat cctcatcctc tggctctact actcggcgat
     1201 gatcctgctc ctgggcgcgg tcacctcggc ggtgcatgac gcccgcgtct ttcacgacag
     1261 ggcggaacat ccccagcgcc ggggctacat cgaaatcgcc gagccctgac cacacccgtc
     1321 gaaagagcaa aaaaacaacg agcaacctat tgacagatcg gcgtcgctgg tgtattatat
     1381 acccgtcacg ccgaagtggc ggaatggcag acgcgctagg ttcagggtct agtgggagta
     1441 gtcccgtgcg ggttcaagtc ccgccttcgg caccagagag aagagacggc gcggtttctc
     1501 aatcccttcg cgaaggggcc agagaatccg cgccgtttct cttgtaccgt ccgccgcgcc
     1561 tccccgccgg cgcggggggg atatccggaa tcccctaccc aggcgcacgc tccatgggcc
     1621 acaccctggt gcccggagcg cagccggcga cccgcatcag ccggtcgccg gtcgccggta
     1681 gcgatgccgc caaacacccc caagccgtct ctcccgggat gcagggagag aggatggcgt
     1741 tgaaacccta ctcgctgtag ttgaagtagg gcgccttgct cccgttgtta tagagcgtca
     1801 cgtgcttcat cgccttcaca tgcccgtccg cgaagcccac gttcgccacg ccgttatgcc
     1861 gttccagcac gttgaagccc ggcctctgat agtcatggta tttatccgaa gacgggaaca
     1921 tcgagaggtg cttccctccg cagatcgagc agttcgagcc gtcagggtcg gtaaccatct
     1981 tgcccctgga gggcgtatgt agccgcccgc ggacattggg atcctgaagc acatcggcca
     2041 ccatcgcgat ctccgctggc ctttgaatct cgctcaagga gactccacaa acgttttcgg
     2101 gcttcttttc gggcatcaga tctatgtggt agccgtagct gcggtggccc aggtaatcac
     2161 ccacc
//
LOCUS       DAOSCV010000805         2163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33401_length_2163_cov_2.796490, whole genome shotgun sequence.
ACCESSION   DAOSCV010000805 DAOSCV010000000
VERSION     DAOSCV010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33401_length_2163_cov_2.796490"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /locus_tag="PLY56_16470"
     CDS             complement(<1..2092)
                     /locus_tag="PLY56_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ23123.1"
                     /translation="MRESEDGMIPTEHRHRLLETLLLLVQQCAVAKSLAEVADSTADL
                     LRSLFSAAAAHVMLRREESGLLETFAYSGVGREVINTRKLALNEGIAGVVLAQSKPVY
                     VADIGSDPRWEWRDELRAAGLESAFLAPLLLDTEPVGMLEVYNPAMPPQGPSPEDESV
                     LMAFAAHAAGALRVVQARQRAQDESDRLRVVLDSIPLPALVIDGADLAHATIELQNRA
                     SREAAGMALAPGTPVQELSTLYAIYPADCTRPYALEEIPALRALQRGEVIYQEEVHIR
                     LPGDRWLVGLASAAPMRRGGKVTGAVVVFEDITHRVREEEKVRRRYDQLRVIYDTATA
                     VSEAEDLAQIYEKALDGLTRALGTDRASLLLLDADQVMRFKAWRGLSEEYRKAVEGHT
                     PWPPDVQDPRPVLIADVARAPESTPLPEVLQTEGIRAIAFIPLVHHHRLVGKLMVYYG
                     EPHVFTDEEVQVAQTIAYHIAYAIERHRTTESLRLSESRYRMLMEQASDPIAVLDAEG
                     QILAANARTAETTGYTREELVGMNVTHLFVPSELSEHPLHLPELRAGKTVVTERQVRT
                     KDGSIRIAEVSARLLPDGRIQAIARDITERKRDAIAQRFLAEASRLLASSLDYETTLS
                     QVARLAVPGLGDWCFVYIFDSGLERTEVATADPADEALAKKLVGFPPPTLKAVCEALP
                     DLCAGEAHLFESVHP"
BASE COUNT          370 a          698 c          714 g          381 t
ORIGIN      
        1 cgggatgcac gctctcgaag agatgtgcct cgccagcgca caggtccggc agggcctcgc
       61 agacagcctt gagcgtaggg ggcgggaagc ctacgagctt tttggccagc gcctcgtctg
      121 ccggatccgc agtggccact tcggtgcgct ccagcccgga gtcgaagata tagacgaagc
      181 accagtcccc gaggccgggc acggccaggc gggccacttg cgagagcgtt gtctcatagt
      241 cgagcgaaga ggcgagtaat cggcttgcct ccgccaggaa acgctgggcg atggcgtcgc
      301 gcttccgctc cgtaatgtcg cgcgcgatcg cctgtatccg gccatctggc aggagcctgg
      361 cgctgacctc agcgatgcga atggaaccat ctttggtgcg gacctgacgc tcagtcacca
      421 ccgtcttgcc ggcgcggagc tcgggcagat gcaaggggtg ttcggacagt tccgaaggca
      481 cgaataagtg ggtgacattc atccccacca actcctcgcg cgtatagccg gtggtctccg
      541 cggtgcgcgc gttggccgcg aggatctgac cctccgcatc caacaccgcg atcggatccg
      601 acgcctgctc catcagcatc cggtagcggc tctcgctcag tcggagggat tcggttgtgc
      661 gatggcgctc aatggcatac gcgatgtggt aggcgatggt ctgggctacc tgtacctcct
      721 catcggtgaa cacgtgcggc tcgccgtagt agaccatcag cttccctacc agccggtggt
      781 ggtggacgag cggaatgaac gcgatcgcac gaataccttc cgtctggagc acctcgggaa
      841 gtggagtgga ttcgggcgcc ctcgccacgt cggcgatgag cacggggcgg gggtcctgaa
      901 catcaggtgg ccaaggcgtg tgaccttcga cagctttgcg gtactcttcc gagaggcccc
      961 gccacgcctt gaagcgcatc acctgatcgg catccagcag caggagcgag gcgcggtcag
     1021 ttcccaaagc gcgggtgaga ccgtcaagcg ccttctcgta aatctgcgcc agatcctcgg
     1081 cctcgctgac ggctgtggcc gtatcgtaga tcacgcgcag ctggtcatag cggcggcgta
     1141 ccttctcctc ctccctgacg cggtgtgtga tatcttcaaa gacgacgacg gcgcccgtca
     1201 ccttgccccc gcggcgcata ggcgctgcgg atgccaggcc cacaagccac cggtcaccgg
     1261 gcaggcggat gtgaacctcc tcctgataga tcacctcgcc gcgctggagg gcacgcaggg
     1321 cggggatctc ttccagggcg taggggcggg tgcagtccgc cgggtagatg gcatagagag
     1381 tgctcaattc ttggaccggg gtgccgggcg caagggccat ccccgctgct tcgcgcgaag
     1441 cccggttttg cagctcgatg gtcgcatggg cgaggtcggc accgtcgatg acgagtgcag
     1501 gcagagggat gctatcgagc acgacccgca agcggtcgct ctcatcctgc gctcgctggc
     1561 gcgcctggac cacgcgaagg gccccggcgg cgtgcgcggc gaaggccatg agcacgctct
     1621 cgtcttccgg gcttgggccc tgtggcggca tcgcagggtt gtacacctca agcatgccta
     1681 ccggctccgt gtcgaggagg agaggcgcca gaaacgcgct ctccagcccg gctgcgcgca
     1741 gctcatctcg ccactcccag cggggatcgg agccgatgtc ggccacgtag accggtttgg
     1801 actgcgccag cactacgccc gcgatcccct cattcagcgc cagcttccgc gtgttgatta
     1861 cctcacgacc aacgccggag taggcgaagg tctccagcag gccgctctcc tcgcgccgga
     1921 gcatcacatg ggccgccgcc gcggagaaaa gcgagcgcag cagatccgct gtgctatcgg
     1981 caacctcggc gagggatttg gcgacggcgc attgctgcac caacaacagg agcgtctcca
     2041 gcagtctatg gcgatgttca gtcgggatca tcccgtcttc ggattcccgc atctcgtcgg
     2101 cctcccgggg gtcctggatc atggcggtga tacccggact cggcttagtt catgcgcgca
     2161 atg
//
LOCUS       DAOSCV010000806         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33425_length_2162_cov_2.711913, whole genome shotgun sequence.
ACCESSION   DAOSCV010000806 DAOSCV010000000
VERSION     DAOSCV010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33425_length_2162_cov_2.711913"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..979
                     /locus_tag="PLY56_16475"
     CDS             209..979
                     /locus_tag="PLY56_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ23124.1"
                     /translation="MARIPIGLELYSVRHDLDRDPRGTLKAVAEMGYEGVEFAGPPKL
                     SGEELRKLLDEFGLVCCGWHTPFAMVQDDKLAETIALNKAVGNQYVIIPGFPGDLTKT
                     RQDWLKLAEFMNNLADKLAEHGMQTGYHNHYTEFQTLDGEWPWDTFFSNTKKEVIMQL
                     DTGNALRGGAESAPILERYPGRAVTVHLKPYKTGLDDPNAGYKPLIGDDSVDWQNVFR
                     LCESIGGTRWYIVEYESDLYPPLEAVKRCLEKLKAMGK"
     gene            1131..1793
                     /locus_tag="PLY56_16480"
     CDS             1131..1793
                     /locus_tag="PLY56_16480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ23125.1"
                     /translation="MAGSWPRGIPLPAGGRVIDLTLPIHAGMPVYPGDPEITIEPVGD
                     GTYAVHRVSLGTHAGTHIDAPAHLDPGGAGVDAIPLAACLGPARVVDCTGLRQITREA
                     LRERAPGLAAGDRLLLRTDWDLLFGKPGYYTEFPGLTLDAARWLAELPIALLGVETPS
                     LSAEEDHGVHQELLAKGIVVVEGLANLACIPAETCWFAALPLPLAGLDGSPARAVAWL
                     DE"
     gene            complement(1782..>2162)
                     /locus_tag="PLY56_16485"
     CDS             complement(1782..>2162)
                     /locus_tag="PLY56_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOJ23126.1"
                     /translation="GLVAPALMHCEEKSLTQIARASKDLIARAKSGGLRPEELTEATF
                     STSNMGMFDVESFSAIIVPPQVAILAIASVQETPVVRNGQVTVAQIMKVTLSADHRAV
                     DGAQAALFLQEVRKRLENPALLLV"
BASE COUNT          363 a          703 c          711 g          385 t
ORIGIN      
        1 cacctccgtc gtcaccattg gcggtgtcct cctctggtag ggtgctccct cttccgcttg
       61 ccgcacgcca cgatggcgag cgacaacgtg gtatgctccc gcgcgcatac tggcggcaag
      121 ggcaggaagg gctctgcccc tagcgaacct cccctctgga gagccgtagc gctggttcga
      181 agcgctgttg cgctccagag gaggaacgat ggcaaggata ccgattggtc tcgagcttta
      241 ttccgttcgg catgacctgg accgtgatcc gcgcggcacg ttgaaggccg tcgcggagat
      301 gggctatgag ggcgttgagt ttgccggccc gccgaagctt tcgggcgagg agctgcgcaa
      361 gctgctcgat gagttcggcc tcgtctgctg tggctggcac acgcccttcg ccatggtgca
      421 ggacgacaag cttgcggaga cgatcgccct gaacaaagcc gtgggcaatc agtacgtgat
      481 catccccggc tttccgggcg acctgacgaa gacgcgccaa gactggctga agctcgccga
      541 gttcatgaac aacctcgccg ataagctcgc cgagcatggg atgcagaccg gctaccacaa
      601 ccactacacc gagttccaga cgctggatgg tgagtggccg tgggacacct tcttcagcaa
      661 cacgaagaag gaagtgatta tgcagctcga taccgggaat gcgctgcgtg gtggcgccga
      721 atccgcgccg atcctggagc gctatcctgg ccgcgcggtc accgtgcacc tcaagccgta
      781 caagaccggc ctggacgacc ccaacgccgg ctacaagccg ctcatcggcg acgatagcgt
      841 cgattggcag aacgtcttcc ggctctgcga gagcatcggc ggcacccggt ggtacattgt
      901 cgagtacgag agcgatctct acccgccgct ggaagccgtg aagcgctgcc tcgagaagct
      961 caaggcgatg gggaagtagg atcgctccgc atggggttgc cttcccagcg gggagaggca
     1021 caccccatgc gggcctgctc ggaccgggtg aacccgcttc gcaagttgat tcgccggaag
     1081 cgagcgcgag ttacctggga tctctccgga acgctttctg aaaggcacgc atggcaggca
     1141 gctggccgcg gggcattcca cttcccgccg ggggccgggt gatcgacctc acgctcccta
     1201 tccacgcggg gatgccggtc tatccgggcg atcccgagat caccatcgag ccggtagggg
     1261 atggcacgta cgcggtgcac cgggtctcgc tgggaacgca cgcgggcacg cacatcgacg
     1321 cgccggcgca tctggatcct ggcggcgccg gtgtggacgc gattcccttg gcggcgtgcc
     1381 tcgggccggc gcgggtggtg gattgcaccg gcctgaggca aatcacgcgt gaagccctgc
     1441 gtgagcgcgc gcctggcctc gcggcgggcg accgcctcct cctgcgcacc gattgggatc
     1501 tcctctttgg caagcccggc tactacacgg agtttcccgg tctcactctg gatgccgcgc
     1561 gctggctggc cgagctgccc attgctctgc ttggcgtgga gacgccctcc ctctccgccg
     1621 aggaggatca cggcgtgcac caggagctgc tggcaaaagg catcgtggtc gtcgaaggcc
     1681 tggcgaatct ggcgtgcatc cccgcggaga cgtgttggtt cgcggcgctc ccgctccccc
     1741 tggccggcct ggatggttcg cccgcgcgcg ccgtggcgtg gctagacgag tagcaacgcc
     1801 ggattttcca ggcgctttcg tacctcctga aggaagagcg cggcctgggc gccatccacg
     1861 gcgcgatggt ctgccgagag cgtgaccttc atgatctgag ctacggttac ctgcccgttg
     1921 cgtacgaccg gcgtctcctg cacgctcgcg atcgccagga tcgccacctg gggcggaacg
     1981 atgatcgcgg agaagctctc cacgtcgaac atccccatgt tgctggtcga aaaagtcgcc
     2041 tcggtcagct cttccgggcg caaccctccc gatttggcgc gggcaatcag atccttgctg
     2101 gcacgagcga tctgcgtgag gctcttctcc tcgcaatgca tcagcgcggg cgcgaccagc
     2161 cc
//
LOCUS       DAOSCV010000807         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33505_length_2158_cov_4.428911, whole genome shotgun sequence.
ACCESSION   DAOSCV010000807 DAOSCV010000000
VERSION     DAOSCV010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33505_length_2158_cov_4.428911"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PLY56_16490"
     CDS             complement(<1..1387)
                     /locus_tag="PLY56_16490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ23127.1"
                     /translation="MAKFIFVTGGVVSSIGKGITTASLGRMLKNRGYKVALQKLDPYL
                     NVDAGTMNPYQHGEVFVTDDGAETDLDLGHYERFVDVSLSRNSNITTGAIYGAVIAKE
                     RRGDYLGGTVQVIPHITNEIKERIHRIAQESGAHVAIVEIGGTVGDIEGQPFLEAIRQ
                     FKKDAGADNVLYVHVTLVPSVGPWSEMKTKPTQHSVMMLRQIGIQPDVLVCRTKAPLS
                     EEMRDKISLFCDVPKEGVVEARDVDSIYEIPLVFEEGGFGEYLVRELGLPGGRPELTA
                     WRRMVETIHRPSRHTRVALVGKYTQLRDAYISVAEALQHGGIANDCGVEIEWVESEDL
                     ERRDPEEAFAGCHGVVVAGGFGVRGIEGKVRAIRWARETGVPFLGLCLGLQCAVIEYA
                     RNACGLDGASSQEFDEDCAHPVIHLLPEQEGVTDRGGTMRLGQWPCRLKEDSLAAKLY
                     KTTEILERHRHR"
     gene            complement(1515..>2158)
                     /locus_tag="PLY56_16495"
     CDS             complement(1515..>2158)
                     /locus_tag="PLY56_16495"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ23128.1"
                     /translation="PASIVGAQIDAVTTDLGVGACKIGWYYSAELTTVIAGRVRRRSL
                     HPVVLDPSLWSERGMALTPPRTIKRIIRELFPVATVVTVTARELKVLSGTPGETAEEL
                     ASAAARIRAMGPQSVVVEDAPAPEGSTVLVFDARGAEQVASGPARGVVAGAGSIFSAA
                     LTAALARGDDLRQGARFAARFLEAAIGNAEVLGKGLPVAATLPVMEHQPPGEI"
BASE COUNT          370 a          756 c          657 g          375 t
ORIGIN      
        1 agcggtggcg gtgccgctcc aggatctcgg tggtcttgta gagcttagcc gccagcgaat
       61 cctccttcaa gcggcaaggc cactggccaa gccgcatcgt tcctcctcgg tcggtcacgc
      121 cctcttgctc ggggaggagg tggatcaccg ggtgggcgca gtcctcatcg aactcttgcg
      181 aagaggcgcc atccaggccg caggcgttgc gcgcatactc gatcaccgcg cattgcagcc
      241 ccaggcacag ccccaagaag ggcacgcctg tctcgcgcgc ccagcggatc gcccggacct
      301 ttccctcgat cccacgcacg ccaaacccgc cggcgacgac cacgccgtgg catccggcga
      361 acgcttcctc tgggtctctg cgctccagat cctcggactc gacccactcg atttcgacgc
      421 cgcagtcgtt ggcgatccct ccatgctgga gcgcctcagc gacgctgata taggcatcgc
      481 ggagttgggt gtacttcccc accagggcaa cccgagtgtg ccggctgggc cggtggatgg
      541 tctcgaccat ccgccgccac gccgtcagct ccggccggcc gccgggcagg cccagctcgc
      601 gcactagata ctcgccgaag ccgccctcct caaaaacgag cgggatctca tagatcgaat
      661 cgacgtcgcg cgcctcgacg accccctctt tcggcacgtc gcagaagagg gagatcttgt
      721 cgcgcatctc ctcgctcaag ggggctttgg tgcggcagac gagcacatct ggctggatgc
      781 cgatctggcg gagcatcatc acgctgtgct gcgtgggctt cgtcttcatc tcggaccagg
      841 ggccgacgct tggcacgagg gtgacgtgga cgtagagcac attgtcggcg ccggcgtcct
      901 tcttgaactg gcgaatcgcc tccaggaacg gctggccctc gatgtcaccc accgtgcccc
      961 cgatctccac gatcgccacg tgggcgccgc tctcctgcgc gatgcggtgg atccgctcct
     1021 tgatctcgtt ggtgatgtgt gggatcacct ggaccgtgcc acccagataa tcgccgcggc
     1081 gctccttggc gatcaccgcg ccgtagatcg cccccgtggt gatgttggag ttgcggctca
     1141 ggctgacgtc cacgaagcgc tcgtaatgcc caagatccag atccgtttcg gcgccgtcgt
     1201 cggtgacgaa tacctcgccg tgctggtagg ggttcatcgt acccgcgtcc acgttgaggt
     1261 acggatccag cttctgcaga gccaccttgt agccgcggtt tttcagcatg cggcccagtg
     1321 acgcggtggt gatccccttc ccgatagatg agacgacccc gccagtcaca aagataaact
     1381 tcgccactcc cgctctcccc tttcaggcct gctccggttt ggaagagatg agccgggcgc
     1441 ctccacggcg tgcgccgcca tgaacaaacc caaagaaggc cccttcgtgc tgaaagaaca
     1501 tagatgccca gccgtcagat ttcaccaggc ggttgatgct ccatgacagg taatgtggct
     1561 gctacgggca gccctttgcc aagtacctcg gcgttgccga tggctgcctc caggaatcgg
     1621 gcagcgaagc gcgcgccctg gcggagatca tcgccccggg ccagcgccgc cgtgagcgcg
     1681 gccgagaaga tcgagccggc gccggccacg acccctcgcg ccggacccga tgccacctgc
     1741 tccgcgccgc gcgcatcaaa aaccagcacg gtgctgccct ccggcgcggg cgcatcctcc
     1801 acaaccacgc tctgagggcc catcgcgcgg atacgcgcgg ccgcggaggc aagctcctcc
     1861 gcggtctctc ccggagttcc gctcaagacc ttcaactcgc gcgcggtaac ggtcacgacg
     1921 gtggctaccg ggaaaagctc gcggattatg cgcttgatcg tgcgcggcgg cgtcaatgcc
     1981 atccctcgct ccgaccaaag agatggatcg aggaccactg gatgcagcga gcgacggcgt
     2041 acccggccag caatcaccgt ggtgagctcg gcggaatagt accaaccaat cttgcacgcg
     2101 cccacgccca gatcggtggt gaccgcgtcg atctgcgcgc ccacgatgct agccggca
//
LOCUS       DAOSCV010000808         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33514_length_2158_cov_2.867808, whole genome shotgun sequence.
ACCESSION   DAOSCV010000808 DAOSCV010000000
VERSION     DAOSCV010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33514_length_2158_cov_2.867808"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLY56_16500"
     CDS             <1..669
                     /locus_tag="PLY56_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ23129.1"
                     /translation="ADQVIALGKGRIRPDSAISIKPISVTGTRQIVRFAFDYAVRYGR
                     KKVTAVAKANIMKFTDGLFYRVAREVAEEYSGKVEYEERLVDNMCMQLVQKPELYDVL
                     VMPNLYGDILSDLCAGLVGGLGVAPGANIGPDVAVFEPTHGSAPRYKGQNKVNPTAFI
                     LSGMLMLRHLGEEDAANRLERAVAAVIAEGKDVTYDMKPNRDDPTAVGTAEMADAIIR
                     KLEE"
     gene            complement(780..>2158)
                     /locus_tag="PLY56_16505"
     CDS             complement(780..>2158)
                     /locus_tag="PLY56_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23130.1"
                     /translation="LDAARAGTQLAPVVMAGKGVTRGLILSPSTRTPGLCANTDIAPT
                     VLHFLGVRPPVQMVGRPLTSQREIHAPETLLALEERLQRVESYARPMMRRLVWLQTAL
                     FGTFLFVAWVYRGALPVHALYAGALGLAALPLAILLTAAILPGSPTSGLLLVLLLDAV
                     LVAVAHSLRRLASPIGILFLASTVVLAVDLCAGAPLMRASALGYSPYGGARYYGIGNE
                     GFGYLAGSLLFAIAFLWPKARRAPSKPGATSHPGTLRIPLLVIAALSLAAAVQVGHPQ
                     LGANFGGAVAAGAASAAAAILALRERLSRRGIILLALGGIAIAALPFLFELLSGGGSQ
                     SHIGRAMDGVREGGLGTAWGIIHRKLAMNWSLLRRSPWSRLLLLAGLTAAYLVHRHGE
                     ALRQRAPVVRATASVAVVAAVAALVANDSGVLPAAAIIVATAAGLLACAARLAMEEPG
                     DAPSQADE"
BASE COUNT          381 a          727 c          755 g          295 t
ORIGIN      
        1 gcggaccagg tgatcgccct tggcaagggc cggatccggc ccgattctgc catttccatc
       61 aagccgatct cggtcaccgg cacccggcag atcgtccgct ttgccttcga ctacgcggtc
      121 cgctatgggc gcaagaaggt gacggcggtc gccaaggcca acatcatgaa gttcaccgac
      181 ggcctcttct accgggtcgc ccgtgaggta gccgaggagt actccggcaa ggtggagtat
      241 gaagagcgcc tggtcgataa tatgtgcatg cagctggtcc agaagccgga gctgtacgac
      301 gttctggtga tgcccaacct atatggcgac atcctctcgg acctgtgcgc cggcctggtg
      361 ggcggcctgg gcgtggcgcc cggggcgaac atcggcccgg atgtggcggt cttcgagccc
      421 acgcacggct cggcgcctcg ctacaagggg caaaacaagg tcaaccccac ggcgttcatc
      481 ctctcgggga tgctgatgct acgccacctc ggagaagagg acgcggcgaa tcgtctcgag
      541 cgcgccgtcg cggcggtgat cgccgagggc aaggatgtga cctacgacat gaagcccaac
      601 cgcgacgatc ccaccgccgt gggcaccgcc gagatggcag acgcgatcat ccgcaagctg
      661 gaggagtaat ccacgttctc cgggcgcatt ggtccgggga acgggttcgg agtgccggca
      721 ccagccggcc aggttcaggt gccgctctcc cctcgggtga gggaagagcg gcacttctgc
      781 tattcgtctg cctgggatgg cgcgtcgccg ggttcttcca tcgccaagcg cgcggcacac
      841 gcgaggagcc cggccgccgt ggccacgatg atggccgccg cgggaagcac tccggagtcg
      901 ttcgccacca gagcggcaac cgcggcgacc acggcgacgc tcgccgtggc ccgcaccacg
      961 ggggcgcgtt gccgcagcgc ctcgccatgc cggtggacta ggtaggctgc cgtgagcccg
     1021 gccaggagga gcagccggct ccagggagaa cggcgcagca agctccagtt catcgcgagc
     1081 ttccggtgaa tgatccccca ggcggttccc aatcccccct cgcgcacgcc atccatcgcg
     1141 cgcccgatgt gcgactgcga tccccctccc gacaggagtt caaagaggaa cggcagtgcg
     1201 gcgattgcga ttccccccag cgccagcagg atgattcccc gccggctcag ccgctctcgc
     1261 agggcgagga ttgccgcggc cgcgctggcc gccccggcgg cgaccgcccc cccgaagttg
     1321 gctcccagtt gcggatggcc cacctggacc gccgcagcca gcgagagcgc ggcaatcacc
     1381 agcagcggga tgcggagagt gcctggatgt gacgttgctc ccggtttcga cggcgcgcgc
     1441 cgcgctttcg gccagagaaa cgcgatggcg aagagcagcg acccggcgag atacccgaag
     1501 ccctcgtttc cgatgccgta gtagcgggcg ccgccatagg gggagtagcc gagcgccgac
     1561 gcgcgcatga gaggggcgcc ggcgcacagg tcgaccgcca ggaccaccgt gctcgccagg
     1621 aagaggatgc ctatcggcga tgccagccgg cgtaggctgt gcgccaccgc aaccagcacc
     1681 gcatccagga gcagcactaa cagcaggccg gaggttggcg aacctggtaa gatcgcggcg
     1741 gtgagcagga tcgccagcgg gagcgccgcc agaccaagcg ccccggcata gagcgcatgc
     1801 acgggcagcg cgccgcgata gacccacgcg acgaagagaa acgtcccgaa gagcgcggtt
     1861 tggagccaga ccaggcggcg catcatcggc cgggcatagc tctccacgcg ctggaggcgt
     1921 tcctctaggg cgagaagcgt ctcgggcgcg tgaatctcac gctgagacgt gagcgggcgg
     1981 cccaccatct gcaccggcgg gcgcacgccg aggaagtgga gcaccgtggg cgcgatatcg
     2041 gtgttggcgc agagccctgg cgtgcgcgtc gagggcgaga ggatcagacc gcgcgtcacg
     2101 cctttcccag ccatgacgac cggggccagc tgggtgccgg cgcgcgccgc gtcgagcg
//
LOCUS       DAOSCV010000809         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33601_length_2154_cov_3.598857, whole genome shotgun sequence.
ACCESSION   DAOSCV010000809 DAOSCV010000000
VERSION     DAOSCV010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33601_length_2154_cov_3.598857"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLY56_16510"
     CDS             complement(<1..770)
                     /locus_tag="PLY56_16510"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ23131.1"
                     /translation="MVAYQGRRVLVTGGTGLIGSRLAERLALEGKAQVRALVHTWSKA
                     AWVSRADVQLVQGDIRDPAAMAEALQGCDVVFHCAAARGGWPLCRATNVEGTRTVLEA
                     AAKSGTARVVHLSCAAVHGPLFAEGADETAPLMRSGIPYIDTKIESEELVRHFSASHG
                     LPATIVRPAPVWGPCSPGFTVMPVRQMQLGRWSLVDGGHGICNAVYIDHLVDALLLAG
                     EREEALGETFLVADDYGITWGEFFEHYRRMVNTGPLPSV"
     gene            complement(764..1774)
                     /locus_tag="PLY56_16515"
     CDS             complement(764..1774)
                     /locus_tag="PLY56_16515"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23132.1"
                     /translation="MPERKLRLAVVGCGAIAERGHIPAARRVGEVRLVALVDSDTARA
                     EALARRFGVPRVAATLSGVAAEVDAAILATPPHVRPALAEEAFREGLHVLCEKPLANT
                     TTEAEAILRAAARARRVLAVAHTYRFFPNRIRLYEMLGQGVLGGVRRIDVREGMPAGW
                     PTRSGYPFRKEMVPGGVLLTLGIHSLDALFWWFGEPFAFQYEDDSVGGLESNARIRMH
                     FAGEVEASVRLSRTCRLENRMTVEGERGALSVPVYDQRVIHQCLGGKEARRVVERGRS
                     GLVHVVAEQLRDFARSITEQRAPRATGADGLRVVQFIEGCYAAKRARPLPEQAPIAGF
                     TW"
     gene            complement(1949..>2154)
                     /locus_tag="PLY56_16520"
     CDS             complement(1949..>2154)
                     /locus_tag="PLY56_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23133.1"
                     /translation="TPKKTVWTLLNTNHRTLRGHLMTVPHRPGARYYDAWNQRPITAN
                     VRGDVAELVFDIGPREVGCIVQQ"
BASE COUNT          344 a          773 c          708 g          329 t
ORIGIN      
        1 accgagggaa gcggccccgt gttcaccatg cggcgatagt gctcgaagaa ctcgccccag
       61 gtgatcccgt agtcgtccgc caccagaaac gtctcgccca gggcctcttc tctctctcct
      121 gccaggagga gggcatccac cagatggtcg atgtagacgg cgttgcagat accgtggccg
      181 ccatccacca gggaccagcg ccccaattgc atctgtcgga ccggcatcac ggtgaagccg
      241 ggcgagcagg ggccccagac gggcgcgggc cggacgatgg tggccggcag cccgtggctc
      301 gcgctgaagt ggcggaccaa ctcctcagat tcgatcttgg tgtcgatgta gggaatgccc
      361 gagcgcatga ggggggcggt ctcatccgca ccctcggcga agagcggacc gtgcaccgcg
      421 gcgcagctga gatgaaccac gcgcgcggtc ccgctcttcg cggctgcttc caggacagtg
      481 cgcgtgccct ccacgttggt ggcgcggcag agaggccatc cgcctctggc ggcagcgcaa
      541 tgaaagacaa cgtcgcaccc ctggagcgcc tccgccatcg ccgccgggtc tcggatatcg
      601 ccctgcacca gctgcacatc ggcgcggctg acccacgcgg ctttgctcca ggtatgcaca
      661 agcgcgcgca cctgcgcctt cccctcgaga gcgagcctct ctgccagccg gctgccgatg
      721 agaccggtgc cgccagtcac cagaacccgt cgcccctggt aggctaccat gtaaaccccg
      781 caatcggtgc ctgctcgggg agcgggcgtg ctcgcttggc ggcgtaacac ccttcgatga
      841 actggacgac gcgcaggcca tcggcgcccg tcgcccgggg ggcccgctgc tccgtgatgc
      901 ttctcgcgaa gtcgcgcagc tgctcggcca cgacgtgcac caggccagac cgccctcgct
      961 cgaccacccg tctcgcctct ttccctccca ggcactggtg aatcactcgt tgatcataca
     1021 ccggcacgct cagagcgccc cgctcgccct ccacggtcat ccggttttcg aggcggcagg
     1081 tgcggctcag gcgaacggag gcctccacct cgccggcgaa gtgcatgcgg attcgcgcat
     1141 tgctctccag gccgcccacg gagtcgtcct catactgaaa agcgaaaggc tcgccgaacc
     1201 accagaagag tgcgtccagc gagtggatcc ccagcgtgag cagaacgccc cctggaacca
     1261 tctccttgcg gaaggggtaa ccggagcgcg tgggccaccc ggccggcatc ccctcgcgga
     1321 catcgatgcg ccggaccccg ccgagaacgc cttgcccgag catctcatac agccggatcc
     1381 ggttggggaa gaagcggtac gtgtgggcga cggcgagcac gcggcgcgcc cgggcggccg
     1441 cgcggaggat cgcctcggct tccgtggtgg tgtttgccag gggtttttcg cagagaacgt
     1501 gcagcccctc gcggaaggcc tcttctgcga gcgctgggcg cacgtgcggt ggcgtcgcga
     1561 gaatcgcggc atcgacctct gccgccacgc cggagagagt ggctgccacg cgcggcaccc
     1621 cgaagcgccg ggccagcgcc tctgcccgcg ccgtgtcgct atccaccagc gccaccaggc
     1681 gcacctcgcc cacccgccgg gccgccggaa tgtggcccct ctcagcgatc gcaccgcacc
     1741 ccacgaccgc cagcctcagc tttctctccg gcatcgttct ccataccccc gcgtcaccat
     1801 cggcaagagc ggtgccaagc agatacgggt acaggggttc gaggcgcgga acgggggaca
     1861 gagtgcctcc tcgggccaga ggcggggccg gcgcggagag gtgggccgag ggagactcgc
     1921 cccctcggcc cgattcgctt gccctgtgtt actgctgcac gatgcagccc acttcacgcg
     1981 gaccgatgtc gaacaccagc tcggccacgt cgccacggac gttcgcggtg atgggccgct
     2041 ggttccacgc gtcgtaatag cgcgctcctg gccggtgggg gaccgtcatc aggtgcccgc
     2101 gcagcgtacg gtggttggtg ttgaggagcg tccacaccgt cttcttcggg gtgg
//
LOCUS       DAOSCV010000810         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33605_length_2154_cov_3.443068, whole genome shotgun sequence.
ACCESSION   DAOSCV010000810 DAOSCV010000000
VERSION     DAOSCV010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33605_length_2154_cov_3.443068"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1637..1798
                     /locus_tag="PLY56_16525"
                     /pseudo
     CDS             1637..1798
                     /locus_tag="PLY56_16525"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1921..2154
                     /locus_tag="PLY56_16530"
                     /pseudo
     CDS             1921..2154
                     /locus_tag="PLY56_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002719879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
BASE COUNT          327 a          686 c          778 g          363 t
ORIGIN      
        1 tatcgagacc atcgccgcgc acgcgatcag ccacggctac acgacgctcg tctggctgga
       61 tagccgtccg aagagctggc tgcgccctga aaactacgcg cagaatgggc gcatcattga
      121 gaagagcgtg gtggggccgc tcaccacgct caaggttggc aaccagccgg tgtcgatgca
      181 tccggagacc tgggtgggct gctacgacgt cgccaagggc cagggcattc tggcagtgag
      241 ccccccaggc gagacgcttc ccttccgtgc cggcgagcag ggggaccgtg gcttcatcgg
      301 gctcaccttc cgcagtctcg ttccggggga ggatcccagc ggcaccggcg cctctgtgac
      361 cggagcgttc accctggtgc ccttctccgg cgaggccaca gcggccgttt caacgtgggt
      421 gaagcgctat ggaaagcagc acatgccgct gcgattcccc cagaaggcgg agagcgggcg
      481 ccggctgcgc gccttgcccg agggcgcgtt atggtgggac ctgcccacag tcaagacctt
      541 cccggatgag ccggcgcctg ccgccacggc atcggccgtg caggtgcagg ccgcgcgcaa
      601 cgaatatgag ccgttccaga tcgtgctccg gcccaagacc gatctggccg gggtccagct
      661 gtcggtttcg ccgctggtgt cgcgccgcgg gaagatcccg gaggagtgcg tctggtggaa
      721 tcccgttgag tattaccgct gcgagcaggc gatggacgcc gccgggtttg cgggcgaggt
      781 gcccgatgcc ctcctgcccg ccgcgccggt cgattgccgg gccgggcgca accagatctt
      841 ctgggtgacg atccgggtgc cgggcgatgc cgagcccggg gagtatcgcg gcagcattca
      901 gttacgggcc ggctcgcgcg agctcgctag cgtgcccgtc atcctgcgcg tgcggagctt
      961 ctcgcttcca gacgagcgct cgctgacagc gtttggtccg ctctggaagg cccacctggt
     1021 gaagcactac ggggcagaga aagcggctca gctttggccg acctacctgg aggacctggc
     1081 cgcgcatcgc ctgggggcgc tgcacccgga ggcgtcgccc gtcgccaagt gggatgagca
     1141 gggcgcgctg actcagatcg atttcaccgc gttcgaccgc gcgatggagg agttctttgc
     1201 gcgctaccgg atgaagtacg tggcgctggg tgattttacg attggctacg gccacatccc
     1261 tcggaacaat cgctttggcc gcgccgagga gatcctcacg ccactgtgga aagcccggtg
     1321 cgagagctac gcgcgcgcgc tggccgcgca ccttcgcgag cgcggctggc atgatcgcgt
     1381 ggtctttagc ctcttcgatg agccgcgcgc cgagtattac ccgctgatcc gcgatacggt
     1441 cgccctcctc aagcaagtgg agccttcgtg gcggttcacc ttctggggaa cttacacgcc
     1501 ggccctggac ggcgcgatcc aggtgtggac cgtgccggcc acctactact cgcccagcct
     1561 ggcccggaag ttgcgtgccc gtggcgatga gatttgggtc tacaatcctc ctggctattg
     1621 cgtagggagg acggcgatgg ccgcgcgcgc caactactgg tgggcgtggc gcgaggagat
     1681 cccgacggtc taccaatgga cgaccaacgc ctggatcgac tggacgggga gcaccacgct
     1741 gtgggatgcc caacggaatg catcctgggt gctgccgggc gaggatggcc cccgcgccac
     1801 gttgcgctgg gagctgacgc gagaggggct ggaggatcac gagtacctca cgctgctgtc
     1861 gcggctggcg aaggaggccg agaagcgcgg gctgaaggtg ccggcggtgg agaaggcgct
     1921 cgccgccgcg cggcgcaagg ccaggcgcgg gcgctggtgg tgttcctgca cggctatggc
     1981 gcggatgggt ccgacctgct gggcctgggc gatgtgctgg gtccgcatct gccgggcgtg
     2041 gctttcctgg ggccggatgc gcccgagcct tgcgtgggca atccctttgg ccggcagtgg
     2101 tttccgatcc cctggctgga cgggtccagc gaacaggcgg cggccgaggg gctg
//
LOCUS       DAOSCV010000811         2150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33721_length_2150_cov_2.399045, whole genome shotgun sequence.
ACCESSION   DAOSCV010000811 DAOSCV010000000
VERSION     DAOSCV010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33721_length_2150_cov_2.399045"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2122)
                     /locus_tag="PLY56_16535"
     CDS             complement(<1..2122)
                     /locus_tag="PLY56_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23134.1"
                     /translation="MVLNGASVTHFFDGWDDYEAPKAKTSSERLEGNMPLAVAWNAAA
                     CVAVGLEPSQLVSYLRQEYEPLADGSARLAAIARIVVDPGKTETVKFVLCATPGEWGK
                     YEAFEAYYDSFPEWFHPRKDVDPRTHLGSAQYRAWPAGPWAPEICRRLYGGWDWCYAP
                     FRRTGDIVGRAELWDYEPVRPFGKTRGKPREEYLAWRKEAFTAGARRAGVAMMFYIPA
                     QIWCEERLARERYADALTTDPKAKTYFDTPWVTGNDNELRVFPYKTSFGEQSYRDMRQ
                     VAEELDLSGFAFDTAGDATRYTGPALPNLDARAWDDQVGVYCAEMVAITHLMDFVHTL
                     KKDGRPLSVVSNPMSNGTYASCFHSDSAMLEGSPWQRGRTDGDHLRWKMGRKTLVWWE
                     GYEIGDFVDLESVKPDQLARVYEGLADFTLLQSLRIGYIPTPNFTQGVGRLVRWLPAI
                     VECVQTGWEPVPATRVPEPLWVTRYGRGLKTLLAIAHETGEPVRGEARILHQRMGGNA
                     LLFSHYDGRALTNRVAKGETVVPVNVPVRTPWLLRAQIEVVNASTVRSAEVRETTSAT
                     GSRLEATLTGAGETALRIRVPAGMRLVALRVDGRPVQARPEGDGVVARAKLGANTRLA
                     AQFASNLLDIQERALLDYPFVKDGKPACAIVVPKGAGEREQLAAFRIQEYFRYWYGRV
                     QESATEVLLPIRESDAPGSGPLVRL"
BASE COUNT          356 a          760 c          687 g          347 t
ORIGIN      
        1 tcaggcgcac cagtgggccg gaccccggcg cgtcgctctc gcgaatcggc aggagcacct
       61 cggtcgcgga ttcctgcacg cgcccgtacc agtagcggaa atactcctgg atccggaagg
      121 ccgccagttg ctcgcgctcg ccagcgcctt tgggcaccac gatggcgcac gccggcttgc
      181 cgtccttcac gaaggggtaa tcgaggagcg cgcgctcctg gatgtccaga aggttcgatg
      241 caaactgggc cgccaggcgc gtattggccc cgagctttgc cctggccacg acgccgtcac
      301 cttccggccg ggcctggacg ggccggccat cgacgcggag cgcgacgagc cgcatgcccg
      361 cgggcacccg aatccgcaac gccgtctcgc cagcgccggt cagcgtggcc tccaggcggc
      421 tgcccgtggc gctggtcgtt tcgcgcactt cagcagagcg gacagtgctc gcgttcacca
      481 cctcgatctg ggcgcgcagg agccacggcg tgcgcaccgg cacgttcacc ggcaccaccg
      541 tctcgccctt cgccacccgg ttggtgagcg cgcggccgtc atagtgcgag aagaggagcg
      601 cgttcccgcc catccgctgg tggaggatgc gtgcctcgcc gcgcaccggc tcgcccgtct
      661 cgtgggcaat cgcgaggagc gtcttcagcc cccgaccgta gcgggtcacc cagagcggct
      721 ccggcacgcg cgtcgcaggc accggctccc agccggtctg cacgcactcc acgatggccg
      781 gcagccagcg gaccaggcgg ccaacgccct gagtgaagtt aggcgttggg atgtagccga
      841 ttcgcaggct ctggagcagg gtgaaatcgg ccaggccctc atagacccga gccagctggt
      901 ccggcttcac gctctcgaga tcaacgaaat cgccaatctc gtaaccttcc caccagacga
      961 gcgtcttgcg tcccatcttc cagcgcaggt gatccccatc agtgcgcccg cgctgccagg
     1021 gactgccttc cagcatggcg ctgtcgctat ggaagcaaga ggcgtaggtg ccgttcgaca
     1081 tcgggttcga gaccaccgag agcggccggc catccttctt cagcgtgtgc acgaagtcca
     1141 tgaggtgcgt gatcgccacc atctcggcgc agtagacacc cacctggtca tcccaggcgc
     1201 gcgcatccag gttggggagc gccggaccgg tgtagcgcgt cgcgtcgccg gcggtgtcga
     1261 aggcaaagcc ggagaggtct agctcctcgg caacctggcg catgtctcga tagctctgct
     1321 ccccaaagga ggtcttgtag gggaagacac gcagctcgtt atcgtttccg gtgacccacg
     1381 gcgtatcgaa gtaggtcttc gccttcggat cggtggtcag tgcgtcggca tagcgttctc
     1441 tggccaggcg ttcctcgcac cagatctggg cggggatgta gaacatcatc gccacgcccg
     1501 ccctgcgggc tccggcggtg aaagcctcct tgcgccacgc gaggtactcc tcgcgcggct
     1561 tgccgcgcgt cttgccaaaa ggacgcaccg gctcgtagtc ccacagctcc gcgcggccca
     1621 cgatatcgcc ggtgcgccgg aaaggagcat agcaccaatc ccagccccca tagagacgcc
     1681 ggcagatctc aggtgcccat ggcccagcag gccaggcgcg atactgcgcg ctgcccaggt
     1741 gcgtgcgcgg atccacatcc ttccgcgggt ggaaccactc cgggaaggaa tcgtagtagg
     1801 cctcgaacgc ttcgtacttg ccccactcgc cgggcgttgc gcagaggacg aacttcaccg
     1861 tctctgtctt gcccgggtcc accacgatgc gagcgatggc tgccaggcgc gcgctaccat
     1921 ccgcaagcgg ctcgtactct tgccgcagat aggagaccaa ctgcgacggc tccagaccga
     1981 cggcgacaca cgcagcggcg ttccaggcca cggcgagcgg catattcccc tccaggcgct
     2041 cggaggaggt cttcgccttt ggcgcctcat agtcgtccca gccatcgaag aagtgcgtga
     2101 cgctcgcgcc gttcaggacc atgcgcgggc ccacctccag ccactgctgc
//
LOCUS       DAOSCV010000812         2141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33952_length_2141_cov_3.310642, whole genome shotgun sequence.
ACCESSION   DAOSCV010000812 DAOSCV010000000
VERSION     DAOSCV010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33952_length_2141_cov_3.310642"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1365)
                     /locus_tag="PLY56_16540"
     CDS             complement(82..1365)
                     /locus_tag="PLY56_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOJ23135.1"
                     /translation="MKENRIHVSLFVVTTLLTIAVVGLLLNIIQRKQEARDRFAHVVP
                     IADLETDPEVWGHNFPDQYDGWKRTAEAYGATEFGGGAHRDKLALNPRLKELYAGYPF
                     SLEYRERRGHARALEDVIGTKRMGDQKPGTCMSCKSSDNVRLWNEIGPEKFFLTPMRE
                     LQHQVSHSVSCLNCHDPKTMDLRPANPAFLAAMKNRNIDLAKATRQEMRTYVCAQCHV
                     EYYFETPDRPILHFPWENGLRVEEMERHYDAIGFADWEHPVSGAKMIKAQHPDYELWS
                     TGIHARAGVACADCHMPFRRVGAQKISDHWVRSPLVNIAHACQTCHRQSEEEMRERVV
                     TIQRRTRELMDRSEVAIIAATRAIAQAKEAGAPESALEEARALHRRAQFRWDFVSSEN
                     SMGFHSPQEAARLLGEAIDFARQAEITVLQARPGR"
     gene            complement(1376..1801)
                     /gene="nrfH"
                     /locus_tag="PLY56_16545"
     CDS             complement(1376..1801)
                     /gene="nrfH"
                     /locus_tag="PLY56_16545"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020477575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOJ23136.1"
                     /translation="MKQWNAISLGVLVGLGSFTFVYAKGYSYLLNDPRTCVNCHVMRD
                     QYDAWQSSSHHTVAVCNDCHTPHALVPKYWVKATNGMRHSAYFTLGNFHEPIRITESN
                     RRVAEHSCVYCHERLVRSIQGHDGSLRCVSCHPRVGHQD"
BASE COUNT          367 a          672 c          685 g          417 t
ORIGIN      
        1 cagctcgccg cgggcatccg ccagcgcgac cgtctggttg gtgttcccga tgtcgatccc
       61 gaggtagtgc aactccatct tctaccgtcc ggggcgcgcc tggagcacgg tgatctccgc
      121 ctggcgcgcg aaatcgatcg cctctcccag gaggcgcgcc gcctcttgcg ggctatggaa
      181 gcccatgctg ttttcggagg agacgaagtc ccatcggaat tgtgcccgcc ggtgcagcgc
      241 gcgtgcttcc tcgagcgccg attcgggcgc gcccgcctcc ttcgcctggg cgatagcacg
      301 cgtcgcggcg atgatcgcca cctccgaacg gtccatgagc tcccgggtgc gccgctggat
      361 ggtcacgacc cgctctctca tctcctcctc actctgccgg tggcaggttt ggcaggcgtg
      421 cgctatgttg acgagcgggc ttcgcaccca gtggtcggag atcttctgag ctccgacccg
      481 ccggaacggc atatggcaat cggcgcaagc gacgccggcg cgcgcgtgga tgcccgtgct
      541 ccatagctcg taatcgggat gctgcgcctt gatcatcttc gccccgctca ccggatgctc
      601 ccaatcggcg aagccgatgg cgtcatagtg ccgctccatc tcctccacgc gcaggccgtt
      661 ttcccacggg aagtggagaa tcggccggtc tggcgtctcg aaatagtatt cgacgtggca
      721 ctgggcacaa acgtaggtgc gcatctcctg gcgcgtcgcc ttcgccaggt caatgttccg
      781 gttcttcatc gcggccagga acgccgggtt ggccgggcgc aggtccatcg tcttcggatc
      841 gtggcagttg aggcagctga cggaatggct cacctggtgc tggagctcgc gcatcggggt
      901 gaggaagaac ttctccgggc cgatctcgtt ccacaggcgc acgttatcgc tgctcttgca
      961 gctcatgcag gtgcccggct tctggtcgcc catgcgcttt gtgccaatga catcctccag
     1021 cgcccgtgca tggccgcggc gctcgcgata ctcgagactg aagggatagc cggcatacag
     1081 ctccttcagc cgtgggttaa gcgccagctt gtcgcgatgc gcgccgccgc cgaactcggt
     1141 cgcgccgtac gcctcggcgg tgcgcttcca gccatcatac tggtcaggga agttgtggcc
     1201 ccacacctcc gggtcggtct ctaagtcggc gattggcacg acatgcgcga agcggtcgcg
     1261 cgcttcttgc ttgcgctgga tgatgttgag gaggagcccc acgacggcaa tcgtcagaag
     1321 cgtggtgacg acgaagaggg aaacatggat ccggttctct ttcacgggcg acctctcaat
     1381 cctggtggcc gacacgggga tggcaggaga cacaacggag cgagccgtcg tgcccctgga
     1441 tactcctgac gagacgctcg tggcagtata cgcagctatg ctcggccacc cgccggttgc
     1501 tttcggtgat gcgaatcggc tcgtggaagt tccccagggt gaagtaggcg ctatgccgca
     1561 tcccgttggt ggccttcacc cagtacttgg gtacaagcgc gtggggcgta tggcagtcgt
     1621 tgcaaaccgc gacggtatgg tggctggagc tctgccaggc atcatactgg tcgcgcatga
     1681 cgtgacagtt cacgcacgtc cgggggtcgt tcaggaggta agaatagccc ttcgcgtaaa
     1741 cgaaggtaaa gctgccgagc ccgaccagga ctcccagtga gatcgcattc cattgcttca
     1801 tccatcaacc atcccatcat gggggtaccc atcgagcggg gtatttgttt acgttcgccg
     1861 aacgggcgct cggttcctct tgggaacaat agcacagatg aaggggccgg aaacggaggg
     1921 cgagatgcgg gcagcgcggg ctgtgcgttc ctgccacggg tcgataacag gccacgcgcg
     1981 tggccaccgc ccggtcggtt ccacgacgga tcctcagctt gacttgctcg cgggcattca
     2041 gtagaatcag cgtgatgagc agccaaaccc cagccgatag agagggcatc gggaagagaa
     2101 ccagtcccct cgacaccccc attcaatacg tgaagggcgt t
//
LOCUS       DAOSCV010000813         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33975_length_2140_cov_3.929017, whole genome shotgun sequence.
ACCESSION   DAOSCV010000813 DAOSCV010000000
VERSION     DAOSCV010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33975_length_2140_cov_3.929017"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /gene="flgG"
                     /locus_tag="PLY56_16550"
     CDS             complement(<1..657)
                     /gene="flgG"
                     /locus_tag="PLY56_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOJ23137.1"
                     /translation="MIRALWTAASGMEAQQLNVDVIANNLANVNTVGFKKSRVDFQDV
                     MYQTLRTAGSPSARGVQVPTGTQVGLGTRPVATQKMFSQGDFTQTGNPLDMVIEGDGF
                     FQVQRPDGTIAYTRDGSLKLDSQGRLVNSDGYVLQPELVIPSGATAISIGSDGTVSVT
                     MPGQAQPQEVGQIQIARFVNPAGLSSAGRNLFLQTAASGEPETGTPGLNGFGTIAQHF
                     V"
     gene            complement(751..1437)
                     /locus_tag="PLY56_16555"
     CDS             complement(751..1437)
                     /locus_tag="PLY56_16555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOJ23138.1"
                     /translation="MVKGLYASASGMVAEITRQEVIANNLANASSSGFRRQLASFRAT
                     APVGGTSPAGPLPLEPGVREASVTTDYSPGTVISTGNPLDVALDGDGFFVIRAPGGLL
                     YTRDGGFRVGPEGRLETQDGDPVLGREGEIIVGRGPVTIAADGTVYSNGVPTGRLQVV
                     EFPEGAGGEIRVVEAPRVLSGYREGSNVNTVREMAAMIAGYRHYEANQRAIQMQDRTL
                     EQAAGEVARV"
     gene            complement(1644..>2140)
                     /locus_tag="PLY56_16560"
     CDS             complement(1644..>2140)
                     /locus_tag="PLY56_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23139.1"
                     /translation="EVWGTATGEALHDLHTGEPLYSRVRLRLQGEVEGVSIAGDPPAT
                     GSGEAEILLESRRVASTRIQPARPPAPEGQEAGLHIHRPASGERVTGELDVDCRVSIP
                     EARYMVLRIDGIPCAAINHPYQHRIDLRHLSAGEHLLEVELYSEAGELLAQARQVFII
                     AGEE"
BASE COUNT          358 a          724 c          682 g          376 t
ORIGIN      
        1 cacaaagtgc tgggcgatgg tcccaaaccc gttcagaccg ggcgtgcccg tttcgggctc
       61 gccggaggca gccgtctgca ggaagaggtt gcggccggcg ctcgacaggc ccgccgggtt
      121 gacgaaacgc gcgatctgga tctggccgac ttcctggggc tgggcctgcc cgggcatagt
      181 gacggaaacg gtgccatctg agccgatgga gatcgccgtc gcgccggagg gaatcaccag
      241 ttcgggctgc aggacgtagc catccgagtt caccagccgg ccctggctat ccagcttcag
      301 cgagccgtcg cgcgtgtagg cgatggtgcc atccggacgc tgcacctgga agaagccgtc
      361 gccttcaatc accatgtcca gcggattgcc cgtctgggtg aaatcgcctt gcgagaacat
      421 cttctgcgtg gcaaccgggc gagttcccag gccaacctgc gtccctgtag gcacctgcac
      481 gccccgcgcg gaggggctcc ccgccgtgcg caacgtctgg tacatgacgt cctgaaaatc
      541 cacacgcgat ttcttgaagc cgaccgtatt gacattggca aggttgttgg caatgacatc
      601 aacgttgagc tgctgtgctt ccatgccgct ggcggcagtc cacaatgctc gtatcatctg
      661 gatacccttc tcggaaacct ggcccccacc gggtggctgg cggtgttacc tcgccagcca
      721 agaaaagtcg gatgccgaag cgcgcgttgc ctagacgcgc gcgacctcac ccgccgcctg
      781 ctccagggtg cgatcctgca tctggatggc ccgctggttc gcctcatagt gccgatagcc
      841 ggcaatcatc gccgccatct cgcggacggt attcacattc gagccttccc ggtagcctga
      901 gagcacgcgc ggcgcctcga cgacgcggat ctcgccgccg gcgccttccg gaaactcgac
      961 cacctggagg cgcccggtag gcacgccgtt tgagtacacc gtgccatcgg ccgcgatggt
     1021 caccgggcca cgaccaacta tgatctcgcc ctcgcgcccg aggacgggat ctccgtcctg
     1081 cgtctccagg cgaccctccg gacccacccg gaagccgccg tctcgcgtat agaggagacc
     1141 gccaggggcg cggatcacaa agaagccgtc gccatccaga gccacatcga gcgggttgcc
     1201 ggtagagatc accgttccag gcgagtagtc cgtcgtcacg gatgcctcgc ggacgcctgg
     1261 ctccagcggc agcgggccgg ccggtgaggt gccaccaacc ggcgccgtcg cccggaagga
     1321 tgccagctgt cgccggaacc cagaggagga ggcgtttgcc aggttattcg caatcacctc
     1381 ttgccgggtg atctcggcca ccattccact ggcagaagcg tagagacctt tgaccatgca
     1441 cactctcctg gccgcggcgc gtggccggcg gccctctgtc tcgtatttcg gcaggcgcgc
     1501 gcagccgcct taagctacgc cccgcggtgt ttggagacgc cgaaagtggg gtagaagaag
     1561 agtggtcaga gcaacacgct tcctttctgg gatcgagggc ggaacttcag gcgggaggtt
     1621 ccgccctctg atgtttgccc ccctcactcc tctccagcga tgatgaacac ctggcgtgcc
     1681 tgagccagca gctcccctgc ttcgctatac agctccacct ctagcagatg ctcgccggcg
     1741 ctcaggtggc gcaggtcgat ccgatgctga taggggtggt tgatggcagc gcacgggatc
     1801 ccatcgatcc gcaacaccat gtagcgtgcc tctggaatgc tgactcggca gtcaacgtcc
     1861 agctcaccgg tcacgcgctc tccggacgcg gggcgatgga tgtgaaggcc cgcttcttga
     1921 ccctccgggg ccggtggccg cgccggctgg atgcgcgtgc ttgccactcg gcggctctcg
     1981 aggaggatct cggcctctcc gctcccggtc gccggaggat cgcctgcgat ggagacgccc
     2041 tccacctcgc cctggaggcg gagacggacg cgcgagtaga gtggctcgcc ggtgtgcagg
     2101 tcatgcagcg cctcgccggt tgccgttccc cagacctcca
//
LOCUS       DAOSCV010000814         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_33982_length_2140_cov_3.179376, whole genome shotgun sequence.
ACCESSION   DAOSCV010000814 DAOSCV010000000
VERSION     DAOSCV010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33982_length_2140_cov_3.179376"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLY56_16565"
     CDS             <1..427
                     /locus_tag="PLY56_16565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ23140.1"
                     /translation="IEAATARGLVISVPLRVEDYRQRSWLLDIPDVTPHEVAEAARAH
                     PKAKFLPVNGMGYLHTAFGKPGEAQGDYALEISRLTAVLQEELQKLIAAVGPDHLVFG
                     SGMPFTVPEVPLLKLELLDLPEDAMEAIRWKNAARLIGQ"
     gene            575..1465
                     /locus_tag="PLY56_16570"
     CDS             575..1465
                     /locus_tag="PLY56_16570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ23141.1"
                     /translation="MPISAVIPNRDGADLLRRTLPPLLRELPTPENEVVVIDDASTDG
                     SVEMLREEFPAVRAIALSENVGFGAACNLGMSEARHSLVLLLNSDMEVTPGSVAMLRQ
                     HLDDPQVFAAGPEYRNPDPSWRAPEHEQARPSYMLGAPAGGGLFRREVFLELGGFDPL
                     YAPFYWEDFDLGWVAWRHGWKVVFEPRCHFWHLESATIRKLFPAGYIERIRTRNRCLF
                     GWKNLLDPWILARFQARSLLRGISGVLRRHSCASLLGVLDSLPRVPAALRRRHLPPDG
                     RGDWAILRETNTSLELLQQL"
     gene            1505..>2140
                     /locus_tag="PLY56_16575"
     CDS             1505..>2140
                     /locus_tag="PLY56_16575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ23142.1"
                     /translation="MLRETPVTFVLPNYRGRPLLERFLPTILEAASQPGLDASVLVVD
                     DASPDDSVEFIRRHFPTVNLLMLRRNVGFARAVNAGVHAAATDLVLLLNTDVAVEPEF
                     LPPLLEAMEDSQVFAAVPRIRRPAAGGTAESAISATFERGLFRLRFLGDAPFQEHDTP
                     FPTLYPVGAAALIRRPLFLQLGGMDPLFRPYYWEDADLGYRAWKAGYQVLCV"
BASE COUNT          335 a          722 c          688 g          395 t
ORIGIN      
        1 catcgaggcg gccacggcgc gcggcctggt gatctcggtg cccctgcgcg tggaggacta
       61 tcgccagcgt tcgtggctcc tcgacattcc cgacgtcacc ccgcacgagg tcgcggaggc
      121 tgcccgggca caccccaagg cgaagttcct cccggtgaac ggcatgggct acctgcacac
      181 cgcgttcggg aagccgggcg aggcgcaagg cgactacgcc ctggagatct cgcgcctcac
      241 cgccgtgctc caggaggagc tgcagaagct gatcgccgcc gtcggcccgg atcaccttgt
      301 cttcggcagc gggatgccgt tcacggtgcc ggaggtgccc ctgctgaagc tggagctcct
      361 cgacctcccc gaggacgcga tggaagcgat ccgctggaaa aacgcggcgc ggctgatcgg
      421 gcaatagcat tccacgacgc cgtgcgtcgc aggaatctcc agcttattgc cgaacagata
      481 tttgcatttc agcacactgt caggagcgcg ggcgcgggtc cggcgtttca acgccctccc
      541 cctgcgcccg cgtggaagga gccttttgtc cagagtgccg atcagtgctg tgattccaaa
      601 ccgagatggg gccgacctgc tccggcgcac gctaccgccc ctgctgcgcg agctgcctac
      661 gcccgagaac gaggtggtcg tgattgatga cgccagcacc gacggcagcg tggagatgct
      721 ccgcgaggag ttccccgccg tgcgcgccat cgcgctctcc gagaatgtcg gcttcggggc
      781 agcctgcaac ctcggtatga gcgaggcccg gcacagcctg gtcctgctcc tcaacagtga
      841 tatggaggtg accccgggat cggtagcaat gctccgccag cacctggatg acccccaggt
      901 gtttgccgcc gggccagagt atcggaaccc tgatccctcg tggcgggcgc cggagcacga
      961 gcaggcgcgg ccttcctaca tgctcggggc gccagccggg ggcgggcttt tccgacgcga
     1021 ggtattcctg gaactcggcg gcttcgaccc tctctacgcg cccttctact gggaggactt
     1081 cgacctgggg tgggtagcct ggcgccatgg atggaaagtg gtcttcgagc ccaggtgcca
     1141 tttctggcac ctggagagcg cgaccatccg caagctcttc cccgccggct acatcgagcg
     1201 gatccgcacc cgcaaccggt gcctctttgg gtggaagaac cttcttgatc cttggatctt
     1261 ggctcgcttt caggcacggt cgctcctccg gggtatcagc ggcgtgctgc gccggcactc
     1321 ctgtgcctcg ctactgggcg tcctcgattc actgccccgt gtccctgcgg ccctgcgccg
     1381 gcgccacctg ccaccggacg gtcgtggcga ttgggccatc ctgcgcgaga cgaacaccag
     1441 cctggaactg ctgcagcaat tgtgagaggg aatcgggcga ccgactatcc ggggggagct
     1501 tgctttgcta agagagacac ctgtgacgtt cgtgctgccc aactaccgcg ggcgtccgct
     1561 cctggagcgc tttctgccaa ccatcctgga agcagccagc cagccgggtc tcgacgcatc
     1621 ggtcttggtt gtggatgatg cgagccccga tgatagcgtg gagttcatcc gccggcactt
     1681 ccccacggtg aacctgctga tgttgcgccg caacgtcggc ttcgcgcgcg ccgttaatgc
     1741 cggggtccac gcggccgcaa cagacctcgt tctcctactg aacaccgacg tggccgtaga
     1801 gccggagttt ctgccgccgc tcctggaagc gatggaggat tcccaggtat tcgccgccgt
     1861 accgcggatt cgccgaccag cagcgggggg caccgcggag agcgcgattt cagccacctt
     1921 cgagcggggt ctcttccgcc tgcgttttct gggtgatgcc ccgttccaag agcatgacac
     1981 gcccttcccc accctctacc ccgtgggtgc ggcggcattg atccggcgcc ccctcttcct
     2041 gcagctggga gggatggacc cactttttcg cccctactat tgggaagatg ccgatcttgg
     2101 ctaccgggca tggaaagccg gctaccaggt gctgtgcgtg
//
LOCUS       DAOSCV010000815         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34001_length_2139_cov_3.595489, whole genome shotgun sequence.
ACCESSION   DAOSCV010000815 DAOSCV010000000
VERSION     DAOSCV010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34001_length_2139_cov_3.595489"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..1419
                     /locus_tag="PLY56_16580"
     CDS             121..1419
                     /locus_tag="PLY56_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ23143.1"
                     /translation="MKGLRQVALGAWLLLACGGVGGANTAEAPWTLEHCIQTAQQQHG
                     SALAAGEEARAAEAELRKAGSSLFYPVVSAYSTHLRGGIEADQAGSVPRNTGTITDEQ
                     YLMTAEVTLYDGGVQKRNVRMARAVRDATLANLRRETQALELRVIESYVQVLRARHAI
                     RVAQQKLESAQAQKEMVLARRDAGTAAGVDVYPIEVQIANARVEKLGAEAELRASGSA
                     LRNAMGLEEGPVPEVAELPETPAEPASLDACIDLARASRPEVIAAQADLERERAVVAL
                     ARLKSRPQLETAARVDRGLGGSTIHSQWLVYATLTWSLFDRSQREESAAGEARIAALE
                     ARYRQLLKDIASEVTQAYIALQGARARAEASEVSVAMARKSLEVAEARYQLGLAIPIE
                     LVDAQIARSTAELQAIQLRYDAFLAHARLRHAMGQESLIP"
     gene            1546..>2139
                     /locus_tag="PLY56_16585"
     CDS             1546..>2139
                     /locus_tag="PLY56_16585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOJ23144.1"
                     /translation="MDLVEIKKLLRETSVFSMLSEEELDDVANRCEEVHFTLGKLVCR
                     AGDEADAFYIVADGRARVVAESDSGPDLTVGMLNRGDHFGEQGLLTTSRRQYTIRAGG
                     DLSLLRLSKTVFEEILEARPALRAYFTKYITETSVRNFLKMCTAFAPMSPAEIRDLLG
                     CIQIVEFGPEEAIIREGEEGDAFYVLRSGSARVVKESM"
BASE COUNT          377 a          654 c          770 g          338 t
ORIGIN      
        1 ggggggttcg cgaaggcctc aaggagggag agcaggtggt ggtcgatggg caggcgatgc
       61 tcaaggacgg gcgcccggtg cgggtgagat agcagaagcg gggagaatca gagcacggct
      121 atgaaggggc ttcgacaggt tgcgctggga gcatggctct tgctggcatg cggcggggta
      181 ggcggcgcca acactgccga ggcgccgtgg acgctggagc actgcatcca aaccgcgcaa
      241 cagcagcacg gcagtgctct ggcggctggt gaggaggcgc gcgctgccga ggccgagctc
      301 cgaaaagcag gttcctccct cttctacccc gtggtaagcg cctactcgac gcacctgcgc
      361 gggggtatcg aggccgatca ggcgggtagc gtgccgcgca acaccggcac gatcaccgac
      421 gagcagtacc tgatgaccgc tgaggtgacc ctctatgacg gaggcgtgca gaagcgcaac
      481 gtgcggatgg cgcgcgccgt gcgcgacgcc accctggcga atctgcggcg cgagacacag
      541 gccctcgaat tgagagtgat cgagagctac gtccaggtgt tgcgcgcgcg gcacgcgatt
      601 cgggtggcac agcagaagct ggagagtgcc caggctcaga aagagatggt cctcgcgcgg
      661 cgcgacgccg gcacggcggc tggcgtggat gtctatccca ttgaagtgca gattgccaac
      721 gctcgggttg agaagctggg cgccgaggca gagctgcgcg cgtctggttc tgcactgcga
      781 aacgcgatgg ggctggaaga aggccctgtg ccggaggtgg cggagttgcc ggaaacgcca
      841 gccgaaccgg cctcgttgga tgcgtgcatt gatctcgcgc gggcgagccg gccggaggtg
      901 atcgcggcgc aggcggatct ggagcgcgag cgggcagtgg ttgctctcgc gcgcctgaag
      961 agccgcccgc agctcgagac ggcggcgcgc gtggatcgcg ggctgggcgg ctccacgatc
     1021 cacagccagt ggttggtcta cgccacgctg acctggtcac tcttcgaccg tagccagcgc
     1081 gaagagagtg ccgccggcga ggcgcgcatc gcggcgctgg aggcgcgcta ccgccagctt
     1141 ctgaaagata ttgcttccga ggtgactcag gcttacattg ctctccaggg cgcccgggcg
     1201 cgcgccgagg cgagtgaggt gagcgtcgcg atggccagga agagcctgga agtggcggag
     1261 gcccgctacc agctcgggct ggcgattccc atcgagctag tggatgcgca aattgcccgt
     1321 agcaccgccg agttgcaggc gatccagttg cgatacgatg cgttcctggc ccacgcccgg
     1381 ctccgccacg ccatggggca ggagagcctg attccatagg cctatccgga gagcccgacc
     1441 acggtggccc gggagcgcca ctctcctccc gcctgctccc gagtattgcg gggcgactgc
     1501 tccctcccgg cgggttcccg gtgtgtggcc agagggatga ccacattgga tctggtcgag
     1561 atcaagaaac tgctgcgcga gacgagcgtc ttctccatgc tctccgagga ggagctggat
     1621 gatgtcgcca accggtgcga ggaggtccac ttcacgctcg gtaagctggt atgccgcgcg
     1681 ggcgacgagg cagatgcctt ctacatcgtt gccgatggcc gcgcgcgcgt cgtggccgag
     1741 agcgacagcg gccccgacct caccgtgggc atgctcaacc gcggcgacca cttcggcgag
     1801 cagggcttgc tcaccacatc acggcgtcaa tataccatcc gcgccggtgg ggatctctcg
     1861 ctgctccgtc tgagcaagac ggtctttgag gagatcctcg aagcacgccc agcgctgcgc
     1921 gcctacttca ccaagtacat caccgagacc tcggtgcgca attttctgaa gatgtgcacc
     1981 gcgttcgcgc ccatgtcgcc ggccgagatc cgcgacctcc tcggctgcat ccagatcgtc
     2041 gagttcgggc ccgaagaggc gatcatccgc gagggcgaag agggagacgc tttctacgtg
     2101 ctgcgcagtg gcagcgcccg ggttgtcaag gagagcatg
//
LOCUS       DAOSCV010000816         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34008_length_2139_cov_2.739923, whole genome shotgun sequence.
ACCESSION   DAOSCV010000816 DAOSCV010000000
VERSION     DAOSCV010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34008_length_2139_cov_2.739923"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1613)
                     /locus_tag="PLY56_16590"
     CDS             complement(246..1613)
                     /locus_tag="PLY56_16590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOJ23145.1"
                     /translation="MAGRDRSWAYVEPGSVPRIVTETVPGPKSQEYNARGMQYMIGYS
                     SQVTLFPVVFEKGYGVTLTDVDGNVYLDFSSGIYVTTLGHCHPKVSEAVAHYAKTLMN
                     CHDFNTPVKVALLEKIAAIQPWDLTGMQLYDSGTTAVEAALRVCRAATGKTEFLSCYM
                     DFHGKTGHAVSLAKMNPTNGAGRAQGFYLVPRPDPYRPLFKKPDGSIDTDAYIQFFDD
                     FITNATTGNVAAFVLEPVQGWAGSIFPPDDFFPKLRRFCDERGILLVADEVLTGMGRT
                     GTYFAMEHWDTRPDVVTLGKGFGNGFPVTAMLVSERYKESINKISASTSYGGNPMACA
                     AALASIEVIEEEGLLENARKLGAYFEQRLAAIKDAHPIVGDVRGKGCLLGVELVKDRG
                     TKEPFVEAGKKVYQYAFRKGLAWVPAGHILRMSPPIVMDVETAAKGMDLIEEAIGEVE
                     AEFGY"
     gene            1787..>2139
                     /locus_tag="PLY56_16595"
     CDS             1787..>2139
                     /locus_tag="PLY56_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ23146.1"
                     /translation="MNERQEGILASIRQGQRVRVRELAERFGVSEMTIRRDLVALEAQ
                     GLLIRTHGGGAPVSRLRAPDGALTPASPEKVAIGRAAAALAAPGETVMVDAGTTALQV
                     ARHLPRDAGLIVVTTS"
BASE COUNT          339 a          691 c          719 g          390 t
ORIGIN      
        1 tttcgcccgg cgcagccacc ggtcggtctc tttgaggaag gcctccagag tgtggcaccc
       61 gtatggcggg cgatgtgaaa tgctggccag cgtcacttct ctgcgcatct gtgtctccct
      121 cgtgccgggc ttcaccgtcc ggcatcacag ggcctattcc atgcgcgtgt tggcggctcc
      181 tgcatcgcgc gagagggacg agcggcgccg gccgcacggg cgtctatgcg gccggcgcgc
      241 cgcgatcaat agccgaactc cgcctcgacc tcgccaatcg cctcctcgat gagatccatc
      301 cccttcgcgg cggtttcgac atccatcacg atggggggtg acatgcgcag gatgtgccct
      361 gccggcaccc aggcgagacc cttgcggaag gcatactggt agaccttctt gcccgcctct
      421 acaaaaggct ccttcgtccc gcggtctttc accagctcca cgccaaggag gcaccccttg
      481 ccgcgcacgt cgccgacgat cgggtgggca tccttgatcg cggccaggcg ttgctcgaag
      541 taggcgccca gctttcgcgc gttttccaga agcccttcct cctcgatcac ctcgatgctc
      601 gccagcgcgg cggcgcaggc cattgggttg ccgccatagc tggtggacgc ggagatcttg
      661 ttgatgctct ccttgtagcg ctcggagacg agcatcgccg tgaccgggaa gccgttgcca
      721 aaccccttgc cgagggtgac tacgtcgggg cgcgtatccc agtgttccat ggcgaagtag
      781 gtgccggtgc gccccatgcc ggtgagcacc tcgtcagcga ccagcaggat gccgcgctcg
      841 tcgcagaagc ggcgcagctt ggggaagaag tcgtccggcg ggaagatgga gccggcccag
      901 ccctgcaccg gctccagcac gaacgcggcg acgttcccgg tggtggcgtt ggtgatgaag
      961 tcgtcgaaga actggatgta ggcgtcggtg tcgatggagc cgtccggctt cttgaagagc
     1021 gggcggtagg ggtcgggccg cggcaccagg tagaagccct gcgcccgccc ggcgccgttg
     1081 gtggggttca tcttcgccag gctcaccgcg tgccccgtct tgccgtggaa atccatgtag
     1141 cacgagagga actcggtttt gccggtcgcc gcccggcaga cgcgcagcgc cgcctccacc
     1201 gccgtggtcc cgctgtcata cagctgcatc ccggtcaggt cccacggctg gatggcggcg
     1261 atcttctcca ggagcgccac cttcaccggg gtgttgaagt cgtggcagtt catcagcgtc
     1321 ttcgcgtagt gggcgaccgc ctcgctcacc ttcgggtggc agtggcctag cgtagtgacg
     1381 tagatcccgc tggagaagtc gagatagacg ttgccatcga catcggtcag cgtcaccccg
     1441 tagcccttct caaagacgac cgggaagagc gtaacctggc tggaatagcc gatcatgtac
     1501 tgcatccccc gggcgttgta ctcctgcgac ttcgggcccg gcaccgtctc cgtgacgatg
     1561 cgtggcacac tccccggctc cacgtaggcc cacgagcgat ctctgccagc catggcgtac
     1621 ctcctgttcg agattgtgcg aatgtgtgcg ttcatgatag caccccatcg gatggttgtc
     1681 aagcgtacgc gggccatgtg gtgctgaaaa tgcgttgagc ctactcttga cgcccttggc
     1741 tacccacggt ataatggtgt tcgaaacgaa cacaacggga gacgggatga acgagcggca
     1801 ggaggggatc ctcgcgagca tccggcaggg gcagcgggtg cgggtccgcg agttggcgga
     1861 gcgcttcggc gtgtcggaga tgacaatccg gcgtgatctg gtggcattgg aggcacaagg
     1921 gctgctcatc cggacgcacg gcgggggcgc gccggtgtcg cgattgcgcg cgccggatgg
     1981 agcgctcacg cccgcctcgc cggagaaagt ggccattggc cgggccgcgg cagcactcgc
     2041 cgcgccgggg gagacggtga tggtggatgc cgggacgacg gcgctccagg ttgcccggca
     2101 cctcccgcgc gacgcgggcc tcatcgtcgt gaccacctc
//
LOCUS       DAOSCV010000817         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34155_length_2133_cov_3.523580, whole genome shotgun sequence.
ACCESSION   DAOSCV010000817 DAOSCV010000000
VERSION     DAOSCV010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34155_length_2133_cov_3.523580"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLY56_16600"
     CDS             <1..243
                     /locus_tag="PLY56_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOJ23147.1"
                     /translation="ALRYDPEKHAAPVVVAKGRLLLAEKIRTIARRHHVPIVRNPPLA
                     RALYRMVKVGHTIPETLFRAVAEVLAFIYRTYGRKF"
     gene            244..>1539
                     /locus_tag="PLY56_16605"
     CDS             244..>1539
                     /locus_tag="PLY56_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOJ23148.1"
                     /translation="MAIAMEKQGAAGVRQVAFGGLLRHSDMAVAVMSVVVVAMMIIPL
                     PPMVLDILLTINIAGSLMILLVCLYSMEPLEFASFPTLILLATLFRLALNISSTRLIL
                     LRGYAGEVIASFGEYVVGGNYIVGLVIFLLLVVIQFVVITQGAGRVAEVAARFTLDAM
                     PGKQMAIDADLNAGLISEDGARARRAKIQQEADFYGAMDGASKFVRGDAIAAIVMILV
                     NTLGGFAVGMFQMKMDLLQALRTFTLLTVGDGLVCQIPALLLSTATGVIVTRAASESR
                     LGQEVVSQTLSQPRAVMIASAMMLALALVPGLPKIPFLVISVALFIIGRALGTETCEA
                     PAERPGEAPGASAPVETAPSSEAPEEIERVLHVDPVEVEIGYGLIRLADPQQGGHLLS
                     RVGLMRRQLAQELGLIVPPVRVRDNAQLRHNTYSIKLRGI"
     assembly_gap    1540..1549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1574..>2133
                     /locus_tag="PLY56_16610"
     CDS             1574..>2133
                     /locus_tag="PLY56_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HOJ23149.1"
                     /translation="MDPGGVASPVQGIQAKEPAFGLPALWVDEQTRQAAELAGYTVVD
                     PLTVLITHLTEIIRRHADELLSRQTVQELLDGVREREPAAVQGLVPDLLSLADVQRVL
                     QRLLHERVSIRDLVPILEALGDAARFTKSIDLLTEEARRALARWLCQTYQAADGKIHV
                     VMLDPRTEQFLTDRIRTSEQGSHLLL"
BASE COUNT          349 a          658 c          710 g          406 t
ORIGIN      
        1 gccctgcgct atgatccgga gaagcatgcc gcgcccgttg tggtggcgaa gggacggctg
       61 ctcctagcgg agaagatccg caccattgcc cggaggcacc atgtgccgat cgtgcgcaat
      121 ccgcccctgg cacgagccct ctaccgaatg gtgaaggtcg gccataccat tccagagacg
      181 ctgttccgcg cggtagccga ggtgctggcg ttcatctacc gcacctacgg aaggaagttc
      241 tagatggcga tagcgatgga gaaacaggga gccgcggggg tgaggcaggt ggcctttggt
      301 ggcctgctgc gccatagcga catggccgtg gcggtcatgt ccgtggtggt cgtggcgatg
      361 atgatcatcc cattgccacc gatggtgctc gacatcctgc tcaccatcaa tatcgccgga
      421 tcgctgatga tcctcctggt gtgcctctac tcgatggagc ccctggagtt cgcatccttt
      481 ccgacgttga tactgctggc aacgctcttc cgcctggcgc tgaacatctc gtctacgcgt
      541 ctgatcctcc tccggggcta tgccggcgaa gtgatcgcct ccttcgggga gtatgtcgtc
      601 ggtggaaact acatcgttgg tctggtcatc ttcctgctcc tcgtggtgat ccagttcgtg
      661 gtgatcaccc agggcgcggg gcgtgtggca gaagtggccg cgcgcttcac cctggatgcc
      721 atgccgggca agcagatggc catcgatgcc gatctgaacg ccggcctcat ctccgaggat
      781 ggggcgcgag cgcgccgtgc caagatccag caagaggccg atttctatgg cgccatggat
      841 ggcgcgagta agttcgtgcg tggtgatgca atcgcggcga tcgtcatgat cctggtcaac
      901 accctgggcg ggttcgctgt cgggatgttc cagatgaaga tggacttgct ccaggcgctg
      961 cgcaccttca ccctcctcac ggtgggtgat gggctggtct gccagatacc ggccctcctg
     1021 ctatcaaccg cgaccggcgt gatcgtcacg cgggctgctt ccgagtctcg cctggggcaa
     1081 gaggtggtca gccagacgct ctcacagcct cgggctgtga tgatcgcgtc ggcgatgatg
     1141 ctggcgctgg cgctggttcc ggggttgccg aagatcccgt tcctcgtgat ttccgtggcg
     1201 ctctttatca tcggaagggc attgggcacc gagacgtgcg aggcgccggc cgagaggcca
     1261 ggagaggcgc ctggagcgtc cgcgcccgtg gagaccgcgc cgagcagtga ggcccccgaa
     1321 gagatcgagc gcgtgctgca tgtggacccg gtcgaggtgg agatcggcta cgggttgatc
     1381 cgcctggcgg atccacagca aggcggccac ctgctcagcc gggtaggcct gatgcgccgc
     1441 cagctagcgc aggaactcgg cctgatcgtg ccgcctgtac gcgtgcgcga caacgcgcag
     1501 ctccggcaca acacctacag catcaagcta cgcgggatcn nnnnnnnnnc tttaccccaa
     1561 gcattacctc gcgatggatc ctgggggcgt agccagcccg gtccagggaa ttcaggcgaa
     1621 ggagcccgcg tttgggctgc cggcgctctg ggtggatgag caaacgcggc aggccgcaga
     1681 gttggctggc tacacggttg tggacccgct caccgttctg atcacgcacc tcaccgagat
     1741 catccggcgg cacgccgatg agctcctcag ccggcagact gtgcaggagc tcctcgatgg
     1801 cgtgcgcgag cgggagccgg ccgccgttca gggcctggtg cccgatctcc tctcgctggc
     1861 ggatgtccag cgcgtgttgc agcgtctgct tcacgagcgc gtgagcatcc gcgacctggt
     1921 tccgatcctg gaagcgctgg gcgatgctgc ccggtttacc aagagcattg acctgctcac
     1981 cgaggaagcg cgccgggcgc tggctcggtg gctctgccag acttaccagg cggctgatgg
     2041 caagatccac gtggtgatgc tggacccgcg aacggagcag tttctcaccg atcgcatccg
     2101 cacctcggag cagggcagcc acctgctgct tga
//
LOCUS       DAOSCV010000818         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34178_length_2132_cov_3.029851, whole genome shotgun sequence.
ACCESSION   DAOSCV010000818 DAOSCV010000000
VERSION     DAOSCV010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34178_length_2132_cov_3.029851"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLY56_16615"
     CDS             <1..660
                     /locus_tag="PLY56_16615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOJ23150.1"
                     /translation="AAALTDACFDFVLGLLRPGITEWEVAAEIECFFRRNGSARESFE
                     TIVASGPLAASPHASATEKPIAAGDLVLMDFGAIYRGYAADITRTVAVGHADDRQREA
                     YAAVLEAQEAAIQAIRPGVRGRDVDQVARDVITARGFGEVCYAHGLGHSLGKHVHDGP
                     ALSRTSEVVLAPGMVITVEPGIYDPEWGGIRIEDDVLVTETGCELLTHSPKEFTVVAA
                     G"
     gene            671..1204
                     /gene="rimI"
                     /locus_tag="PLY56_16620"
     CDS             671..1204
                     /gene="rimI"
                     /locus_tag="PLY56_16620"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOJ23151.1"
                     /translation="MRISVERMQLSDLNDVLRIERASYPTPWRPETFRYEICDNPNAY
                     YVVARVHLPEGPVTVGYAGMWLIIDEAHITTIAVDPNYRGLKIGERLLLTLLTAATQK
                     GACRATLEVRESNLVAQRLYLKYRFGAAGMRRGYYTDTGENAIIMWIENLHDPGLQRF
                     MNERRRCLEEQGALARC"
     gene            1185..>2132
                     /gene="tsaD"
                     /locus_tag="PLY56_16625"
     CDS             1185..>2132
                     /gene="tsaD"
                     /locus_tag="PLY56_16625"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ23152.1"
                     /translation="MRLLGVETSCDETSAAVVEDGRRILTNIIASQVDVHARFGGVVP
                     EIASRHHVESIGAILAAAVADAGTGFDGIDAVATTAGPGLQGALLVGLTAAKVVAFTR
                     GLPLVGVHHIHGHVAANFLTDEPIAFPALCLVVSGGHSDLIIVRSEVDLEPIGHTRDD
                     AAGEALDKGARVLGLGYPGGPIIDRLAQQGDPTAVHFPRALLPGTHDFSFSGIKTALL
                     REVQSGSGHRVEDLAAAYQQAIVETLVQKTIAAARETGVRQVLLAGGVASNSELRAEM
                     QKACAAEGLPLHMPPRSLCTDNAAMIAAAGYHLLRAGRVS"
BASE COUNT          343 a          663 c          747 g          379 t
ORIGIN      
        1 gcagccgcgc tcaccgatgc gtgcttcgac tttgttttgg ggcttctgcg cccgggcata
       61 accgagtggg aagtggccgc cgagatcgag tgcttcttcc gacgcaacgg cagcgcgcgc
      121 gagtcgttcg agacgatcgt ggcatcgggg ccgcttgccg ccagcccgca cgccagcgcg
      181 acggagaagc cgattgcggc gggcgatctg gtgctgatgg actttggggc gatctatcgc
      241 ggctacgccg ccgatatcac gcgaacggtt gccgttgggc atgcggatga ccgccagcgc
      301 gaagcgtacg cggccgtgct cgaggcgcag gaagcggcca tccaggcgat ccggccgggc
      361 gttaggggcc gcgatgtgga tcaggtggcg cgcgacgtga tcaccgcccg cggtttcggc
      421 gaggtatgct atgcccatgg cctgggccac agcctcggga agcacgtaca tgatggtccg
      481 gcgctctcgc gcacgagcga ggtggtgctg gcgccaggga tggtgatcac cgtggagcca
      541 ggcatctacg acccggagtg gggcggcatc cggatcgagg acgatgtgct cgtgacggag
      601 accgggtgcg agttgctgac gcattcaccg aaggagttca cggttgtcgc ggccgggtag
      661 gaggggtccc atgcgcatct cggtggagcg gatgcagcta tcagatctca atgatgtcct
      721 gcgcatcgag cgcgccagct atccgacgcc ctggcggccc gagacgtttc gctacgagat
      781 ctgcgacaac ccgaacgcct actacgtagt ggcgcgcgtc cacctcccag agggccccgt
      841 cacggtaggc tatgccggga tgtggctgat tatcgatgag gcgcacatca ccacgatcgc
      901 cgtggatccc aactaccgcg gcctcaagat tggcgagcgc ctcctgttga cgttgttgac
      961 cgccgcgacc cagaagggcg cctgccgggc gactctcgag gtgcgcgagt ccaacctggt
     1021 ggcgcaacgg ctctatctca aataccgctt tggagccgcc ggcatgcgcc gcggctacta
     1081 tactgacacg ggtgagaatg ccatcatcat gtggattgaa aacctgcacg atcccggatt
     1141 gcagcgcttc atgaacgagc gccggcgttg tttggaggaa cagggtgcgc ttgctcggtg
     1201 ttgagacgag ctgcgacgaa acctccgccg ccgtggtgga ggacggtcgc cgcatcctca
     1261 ccaatatcat cgcttctcag gtcgatgttc acgcacgttt cggcggcgta gtgccggaga
     1321 tcgcttcgcg ccatcatgtg gagagcatcg gtgctatcct tgcggccgcg gtggcggatg
     1381 ccggcaccgg ctttgatggg atcgatgctg tggcgaccac ggccggcccc ggcctccagg
     1441 gggcgctcct agtggggctg accgcggcaa aggtggtcgc cttcacgcgt ggactgcccc
     1501 tggtgggcgt tcaccacatc cacgggcatg tggccgccaa tttcctcacg gacgagccga
     1561 tcgcgtttcc tgcgctgtgc ctggtcgttt caggcgggca cagcgacctg atcattgtgc
     1621 gcagcgaggt ggatctcgag ccgattgggc acacgcgcga cgacgcggca ggcgaggccc
     1681 tggataaggg cgcgcgcgtt cttggcctgg gctaccctgg aggcccgatc atcgaccggt
     1741 tggctcagca gggggatccg accgctgttc atttcccgcg cgcgctgctc ccgggaacgc
     1801 acgacttcag cttcagcggg atcaagacgg cgctgttgcg cgaggtgcag tcgggctctg
     1861 gccaccgggt ggaggacctg gcggcggcct accagcaggc gatcgtggag accctggtgc
     1921 agaagaccat tgccgcggcg cgcgagaccg gcgtgcggca ggtgctcctg gccgggggtg
     1981 tggcatccaa ctcggagctg cgtgccgaga tgcagaaggc gtgtgccgcc gagggccttc
     2041 ccctccacat gcctccgcgt agcctgtgca ctgacaacgc cgccatgatt gctgcggcgg
     2101 gctatcacct cctgcgcgcc ggcagagtat cc
//
LOCUS       DAOSCV010000819         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34277_length_2128_cov_3.804149, whole genome shotgun sequence.
ACCESSION   DAOSCV010000819 DAOSCV010000000
VERSION     DAOSCV010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34277_length_2128_cov_3.804149"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLY56_16630"
     CDS             complement(<1..422)
                     /locus_tag="PLY56_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23153.1"
                     /translation="MDVLLIGRDRRIVEMARKVCAGLAQSPRLQVRDSDEAAQYLFPD
                     NGVSEPHLPALIVMDLNGSGTGEVDLLARTKADPWLRIVPFVVISASPDTADLRRSYL
                     AGANSYVVRPESDQEMEATLRHLFRYWLDVNEPPPPAVG"
     gene            556..>2128
                     /locus_tag="PLY56_16635"
     CDS             556..>2128
                     /locus_tag="PLY56_16635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ23154.1"
                     /translation="MQTQPESPLRRRNGFAQLIAARPASLRERWEAALAPSTAALSPD
                     ERAAVRQIISHFSQACVGYLEHRSEEILASAAAWLGPSGMIRRFTLAQLQEFVGAFHQ
                     AAWPLVALHANGDVSALLEAAEAITDCSLMAIRAVACEHQRRILNEANETAEFGFTLA
                     EVTRSVTSSLNLDEVLQLVCEEGARLTAVEGTLVRLLEEDGTLACRAVWGSGLEATLG
                     SMVFADDTQSLCSRAVQSRRVQTATTTRELPLDPNPMALVPGRACLAVPIIAKGEVLG
                     AMLFLGVQNHGPFDLVRDAELVLTAELLVGQIALAIYNARLHAQVRSLVYALDHIGEC
                     VLVVDSEGRIIYANQAVMRALDHSPSELVGQSLLVLASEAAEEEATRRIIRESFMETR
                     EGEVIWRHRSGAPVPFHVVSTPMGGEGTRSSQVVILARDITEEKRRQEEEKAHRTQLQ
                     CKNQELEAFVYTVSHDLRSPLVSLHGLTGMLRIEAGDALNESARHLLDRLDANVLRME
                     RLIGDLLQFSRVGRHL"
BASE COUNT          366 a          713 c          677 g          372 t
ORIGIN      
        1 ccgaccgccg gcggaggcgg ctcgttgaca tccagccagt agcggaacaa gtgccgcagc
       61 gttgcttcca tctcctggtc ggattccggc cgcacgacgt agctgttggc gcccgccagg
      121 taactgcggc gcagatctgc ggtatcgggc gacgccgata tcaccacgaa gggcacgatg
      181 cgcagccagg gatccgcctt ggtgcgggca agaagatcga cctcgcccgt tccggatcca
      241 ttcaggtcca tgacgataag cgccggcaga tggggctccg agacgccatt gtccgggaaa
      301 agatactggg cggcttcatc actgtcccgc acctgcagcc gtggcgattg tgcgagcccg
      361 gcacacacct tccgggccat ctccacaatc ctgcgatccc ggccaatgag cagcacatcc
      421 atctttccac ggtcccacca ttcttgagct tcctttcggc aggccaccgg tagggcttaa
      481 gcaccggtgg cggattgtac aagtctctaa agccactcgc ttcccctgcc gaacaggaga
      541 ttaggagcct atgtgatgca gacacagcca gagtcacccc tgcggcgaag gaacggcttc
      601 gcccagctga tcgccgcgcg ccccgcgagc ctgcgcgagc gctgggaggc agcgctcgct
      661 ccaagcacgg cggcgctctc gcctgatgag cgcgccgcgg tgcgccagat catctcgcac
      721 ttctcccagg catgcgtggg ttacctggag catcgctccg aagagatcct tgccagcgcc
      781 gcggcatggc tggggccgtc cggcatgatc cggcggttta ccttggcaca gctccaggag
      841 ttcgtcggcg ccttccatca ggccgcgtgg cccctcgtcg ctcttcacgc caacggggac
      901 gtgagcgcac tcctggaggc ggcagaagcc atcacggatt gctcgctcat ggcgattcga
      961 gccgtggcgt gcgagcacca gcggcgcatc ctcaatgagg ccaacgagac cgccgagttc
     1021 ggcttcacgc tggccgaagt gacccgctcc gtcacatcca gcctcaacct cgacgaggtt
     1081 ctccagctag tctgcgagga gggcgcccgc cttaccgccg tggaaggcac actggtccgc
     1141 ttattggagg aggatggaac gctcgcctgc cgggcggtgt ggggatccgg actggaagcc
     1201 acgctcggct ccatggtgtt cgccgacgac acccagtcgc tctgcagtcg cgccgtgcag
     1261 tctcgccgcg ttcaaacagc gacgaccacg cgcgagctgc cgctcgatcc caatccgatg
     1321 gcgctcgttc cgggccgggc ctgcctggcg gtgccgatca tcgccaaggg agaagtgctc
     1381 ggcgccatgc tcttcctggg tgtccagaac cacggcccgt tcgatctcgt tcgcgacgcg
     1441 gagctggtac tcaccgccga gctgctggtg ggccagatcg ccctggcgat ttacaacgct
     1501 cgcctccatg cccaggtgcg cagcctggtc tacgcgctgg accacatcgg cgagtgcgtg
     1561 cttgtcgtcg atagcgaggg ccgaatcatt tatgcaaacc aggccgtgat gagggcgctg
     1621 gatcattccc cctcagagtt ggtggggcag tcgctgctgg tgcttgcctc tgaggcagct
     1681 gaggaggagg ccacccgtcg tatcatccgc gagtcgttca tggaaacgcg agagggcgag
     1741 gtgatctgga ggcatcgcag cggggccccc gtgccgttcc acgtcgtgag cacgccgatg
     1801 ggcggcgaag ggacgcgcag ctcccaggtg gtcatcctgg cccgtgatat caccgaggag
     1861 aagcgccgcc aggaagagga gaaggcgcat cgcacccagc tccaatgcaa gaatcaggag
     1921 ctcgaggcgt tcgtctacac cgtctctcac gatctccgct cgcccctggt ctcgcttcat
     1981 ggcctcacag gcatgctccg catcgaggct ggcgacgcgc tcaacgagag cgcgcggcac
     2041 ctgctggacc gccttgatgc gaacgttctg cgcatggagc ggctgatcgg cgatctgctc
     2101 cagttctccc gcgtgggaag gcacctcc
//
LOCUS       DAOSCV010000820         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34304_length_2127_cov_3.344595, whole genome shotgun sequence.
ACCESSION   DAOSCV010000820 DAOSCV010000000
VERSION     DAOSCV010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34304_length_2127_cov_3.344595"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="PLY56_16640"
     CDS             complement(<1..1435)
                     /locus_tag="PLY56_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOJ23155.1"
                     /translation="MAAEAQAWERWEAGSRFRTLAVVACAVWAAGLLALFASRQVPMG
                     EIRGRVTAEDTGQPVPKAKLLLALASPSVPGRKRQLLVDERGYFHLRRIPAGEYFVSA
                     SSRAHRLPPTRLTIAEGRATEVALELAPTTPFLDVYVPQHVFVPDEPVQITAHGFTRE
                     DALTVEAYRVDPLRVMREARGSLWRALVPNAASDAFGPEERVALRQVAGAAPVARFSH
                     VPARDGEGVFTQRITLPLKDPGLYVVACQGDGMERIDWVMVTGLGLITKRSGSRVLAF
                     AVDLATGEPAPGTEIRVYAEDGERQSMVADEQGIASFQAGESDLLLAARRGNSMAFIR
                     LWGSGAQESGEQQRVFLYTDRPVYRPGARVRFKGIARAVRGEEFLVPEGKPVRVEVHD
                     PRGTLIYAAERMTNDFGSFHGELELSPEASTGTYTLSAEWGGQRHDEYFDVAAYRKPE
                     YQVEVIPERAEYTRSESGWVTIEARYYF"
     gene            complement(1425..>2127)
                     /locus_tag="PLY56_16645"
     CDS             complement(1425..>2127)
                     /locus_tag="PLY56_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23156.1"
                     /translation="WRDLVRASALRWQRPASDESADPAPVALFLHQLLAVGLLALLFR
                     LFLERYRPLATDLSLTVHFTFVGLVAGALIPLWIAESLERRAAYVRLASAPAVRGTAV
                     CAGLLAAAGIAFWAVGAPAAVGIVWGMRSLVGLLAGLLVAGVFLLFPRGERGKGIGAQ
                     ASLLATVLIALATVQLAHLLEPLGVLHREVRVTVLGVFAVLAVLSMLLGQWSARGACK
                     AAFRLEGEGEGTDGR"
BASE COUNT          359 a          795 c          698 g          275 t
ORIGIN      
        1 cgaagtagta gcgcgcctcg atcgtcaccc accccgattc gctgcgcgtg tactccgcgc
       61 gctcggggat cacttccacc tgatactccg gcttccggta ggcggccacg tcgaaatact
      121 cgtcgtgcct ctggcctccc cactccgccg aaagggtgta ggtgcccgtg ctcgcttccg
      181 gcgagagctc cagctcgccg tggaacgagc cgaagtcgtt cgtcatgcgc tcagcagcgt
      241 aaatcagcgt gccgcgggga tcgtggacct ccacgcgcac cggcttccct tccggcacca
      301 ggaactcctc gccgcgcacg gcgcgcgcga tccctttgaa ccggacgcgc gcacccgggc
      361 gatagaccgg ccggtccgta tagaggaaaa cccgctgctg ctccccgctt tcctgcgccc
      421 cgcttcccca gagccggatg aacgccatcg agttaccccg gcgcgcggcc agcaggaggt
      481 cgctctcgcc agcctggaag gatgcgatgc cctgctcatc tgccaccatg ctctggcgct
      541 cgccgtcctc ggcatagacg cggatctccg tgccgggcgc cggctcgccc gtggcgaggt
      601 caacggcaaa agcgagcact ctggagccgg atcgtttcgt gatcaggcca aggccggtga
      661 ccatcaccca gtcaatccgt tccatgccgt cgccctggca ggcgaccaca tagagccccg
      721 gatccttcag gggaagcgtg atccgctggg tgaagacgcc ctctccatcg cgcgcgggaa
      781 cgtgcgagaa ccgcgccacc ggagcggctc ccgcgacctg gcgcagcgcc acccgctcct
      841 ccgggccgaa ggcgtcgctc gcggcattgg gaacgagcgc gcgccacagc gagccgcgcg
      901 cctcgcgcat cacccggagc gggtccaccc gatacgcctc cacggtgagc gcatcttccc
      961 tcgtgaagcc gtgcgcggtg atctgcaccg gctcgtcagg cacgaagacg tgttggggca
     1021 cgtagacatc aaggaaaggc gtggtggggg caagctccag ggccacctcg gtcgcccggc
     1081 cctcggcaat cgtcagccgt gtgggcggga gacggtgggc ccggctggac gcggagacga
     1141 agtactcccc cgcggggatc cggcgcaggt ggaagtagcc gcgctcgtcg acgagcagct
     1201 ggcgcttgcg ccccggcacc gaggggcttg ccagcgccag caggagcttt gcttttggaa
     1261 ccggctggcc ggtatcctcg gcggtgacgc gcccccggat ctcgcccatc ggcacctgcc
     1321 gcgaggcaaa cagcgccagg agacccgccg cccacacggc acacgccacg actgccagcg
     1381 ttcggaagcg gctccccgct tcccagcgtt cccatgcctg cgcctcagcg gccatcggtt
     1441 ccctccccct cgccctccag cctaaacgcg gctttgcacg cgccgcgcgc gctccactgg
     1501 cccaggagca tcgagaggac ggcgagcacg gcgaagacgc ccaggaccgt cacccgaact
     1561 tcgcggtgga gcacgccgag cggctccagc aggtgggcaa gctgcacggt cgccagggcg
     1621 atgagcaccg tcgcgaggag cgacgcctgc gcgccaatcc ccttcccccg ctcccctcgc
     1681 gggaagagca agaagacgcc ggcgaccagc aatccggcga gcaggccgac gagtgaacgc
     1741 atcccccaga caatcccaac cgccgcggga gcgcccacgg cccagaacgc gatccccgcc
     1801 gccgccagaa gccccgcgca aaccgccgtc ccccgcaccg cgggggccga agccagtcgc
     1861 acgtacgccg cccggcgctc taggctttcc gcaatccaga gggggatgag ggcaccggcg
     1921 accagcccca cgaaggtgaa gtggaccgtg agactgagat cggtggccag cgggcggtat
     1981 cgctcgagga agagccggaa gaggagcgcg agcaatccca ccgcgaggag ctggtgcagg
     2041 aagagcgcca ccggggccgg atcggcgctc tcgtccgacg ccgggcgctg ccaccggagc
     2101 gcgctggcac gcaccaggtc acgccag
//
LOCUS       DAOSCV010000821         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34332_length_2126_cov_2.895703, whole genome shotgun sequence.
ACCESSION   DAOSCV010000821 DAOSCV010000000
VERSION     DAOSCV010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34332_length_2126_cov_2.895703"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1571
                     /locus_tag="PLY56_16650"
     CDS             <1..1571
                     /locus_tag="PLY56_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23157.1"
                     /translation="KDGDGWNSLHTQVHRGLKSTGRKDLWTFFDPAVRVPSLWGSGGQ
                     VDYVSQWTYSYPDAIKIGQATDELFAMADGQPGQQVMKMTQVIWYRSGTAPNLPEDES
                     KRAPWEKEKPEARFITISPDHMREAFWSKLSRPIRGIMYHGWGSLVETGEKQGYCYTN
                     PETEKVLGELTRTVVRPLGPTLLNVPDRKSDVALLESFSSQVFAARGTHGWSNAWEAD
                     MHLILQWAQLQPRILYEQTIQRDGLDGYRVLVMPFCDVLPESVVKKVVEFQRKGGIIV
                     ADEFLTPALTPDIVLPSYRRTKKAHEDKAALQALAAQLRRELDPFYTRYGEASDPDVV
                     VRFRQYGQNDYLFAVNDKRTYGDYVGHHGLVMEKGLPHAATITVRRPAGYVYDLVAHR
                     AVPAKATDGTLKIDASFGPGDGRLFLITPKPVTSVRVATAGQARLGSSVAIRAAVLDN
                     QGKPLAAVIPVQVEVIDAQGRPAEPSGYYAAKDGQFSFTLDLARNDTPGRWTVRVREL
                     ASGKTNTATLTVAR"
     gene            1738..>2126
                     /locus_tag="PLY56_16655"
     CDS             1738..>2126
                     /locus_tag="PLY56_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23158.1"
                     /translation="MRVVTLIAFAFLVLSAGRVEAAPVGPIPLRPAVALYVVCDGTPC
                     EARIVVRQGPRPTPERLMARVFTPDERLLFWRYVEYVPAEERAGVTAEGIELRDAANP
                     PKPGDLLLDETVRLDAAGIYQIRLVGGA"
BASE COUNT          355 a          711 c          713 g          347 t
ORIGIN      
        1 ggaaggacgg cgatggctgg aactcgctgc acacccaggt gcatcggggc ttgaaatcca
       61 cgggcaggaa ggatctctgg accttcttcg atccggcagt gcgcgtgccg agcctctggg
      121 ggagcggcgg gcaggtggat tacgtctcgc aatggaccta ctcctacccg gacgcgatca
      181 agatcgggca ggcaacggac gagctgttcg ccatggccga tggccagccc gggcagcagg
      241 tgatgaagat gacccaggtc atctggtacc gcagcggcac cgcgcccaat ctgcccgagg
      301 atgagtcgaa gcgcgccccg tgggagaagg agaagcccga agctcgcttt atcaccatct
      361 cgccggacca tatgcgcgag gcgttctggt ccaagctctc tcgaccgatc cgcggtatca
      421 tgtaccatgg ctggggctct ctggtggaga ccggcgagaa gcagggctac tgctacacga
      481 accctgagac agagaaggtc ctgggcgaac tgacgcgaac cgtggtccgc ccgctcgggc
      541 ccacgctgct caacgtgcct gaccgcaaga gtgacgtggc tctcctggag agcttctcgt
      601 cgcaggtctt cgctgcccgc ggcacccacg gctggagcaa cgcgtgggaa gccgacatgc
      661 atctgatcct ccagtgggct cagctccagc cccggatcct ctacgagcag accatccagc
      721 gcgatggctt ggatggctac cgcgtgctgg tgatgccgtt ctgcgatgtg ctccccgaga
      781 gcgtcgtcaa gaaggtggtc gagttccagc gcaagggtgg gatcatcgtc gcggacgagt
      841 tcctgacccc cgccctgacc cctgatatcg tgctgcccag ctaccggcgc acgaagaagg
      901 cgcatgagga caaggccgcg ctccaggcgc tggccgcgca gctccgacgc gagctcgatc
      961 cgttctacac ccggtatggc gaggcttccg atcccgacgt ggtggtgcgc ttccgccagt
     1021 acgggcagaa cgactacctc ttcgccgtga atgacaagcg cacctatggc gactacgtgg
     1081 gccaccacgg cttggtgatg gagaagggcc tgccccacgc cgcgacgatt acggtgcgcc
     1141 gcccggcagg gtacgtctac gacctcgtgg cccatcgggc cgtgccggcg aaagccactg
     1201 atggcaccct gaagatcgat gccagcttcg gccccgggga tggtcggctc ttcctgatca
     1261 cgcctaagcc tgtgacgagc gttcgggtcg cgacggccgg gcaagcccgg cttggcagct
     1321 ccgtggccat ccgcgccgct gtcctcgaca accagggcaa gccacttgcg gcggtcatcc
     1381 ctgtccaggt ggaggtgatc gatgcccagg ggcggcccgc ggagccgagc ggctactacg
     1441 cggccaagga tggccagttc agcttcacgc tcgacctggc acgcaatgac acccctggcc
     1501 gctggacggt gcgagtccgc gagctggcat ccggcaagac aaacacggcc actctgaccg
     1561 tagcgcgata ggccgcgctc ctcattgcca ggtgtccccg tcgcgtgacg gggacacctg
     1621 ccgatcgtgc cgagcggggt gaaggtctgc cgcgcgtcct aaccatcggg gcgcggatgc
     1681 aacccttcac cccgctcgca agcgccggcg ctaccgggcc ggtggagggt gagaaccatg
     1741 cgcgtggtca cgctcatcgc gttcgcgttt ctggtgctct cagccgggcg ggtcgaagcc
     1801 gcgcccgtgg gcccgatccc gctccgtccg gccgtcgcgc tctatgtggt gtgcgatggc
     1861 acgccgtgcg aggcgcgcat cgtcgtgcgc cagggccccc gacccactcc agagcgcctg
     1921 atggcgcgcg tcttcactcc cgacgagcgg ctccttttct ggcgctacgt ggagtatgtg
     1981 cccgccgaag agcgggccgg cgtcacggcc gaggggatag agctgcgcga cgcggccaat
     2041 cccccgaagc caggcgatct gctcctggat gagaccgtcc ggctggatgc tgccggcatc
     2101 taccagatcc gcctggtggg cggtgc
//
LOCUS       DAOSCV010000822         2116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34541_length_2116_cov_2.653566, whole genome shotgun sequence.
ACCESSION   DAOSCV010000822 DAOSCV010000000
VERSION     DAOSCV010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34541_length_2116_cov_2.653566"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLY56_16660"
     CDS             <1..291
                     /locus_tag="PLY56_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ23159.1"
                     /translation="NYVIPGNDDAIRAIRLITSKMAEAVLEGRPIKEEFVPAEEEVAR
                     AEAEEAAEGAEEEAAEGEAWEPEETVAMGPSADEIEASFAAEVEEEGGIEAE"
     gene            667..1269
                     /gene="tsf"
                     /locus_tag="PLY56_16665"
     CDS             667..1269
                     /gene="tsf"
                     /locus_tag="PLY56_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ23160.1"
                     /translation="MAVTAAEVRELRERSGAGMMDCKRALEETGGDLEKAMTLLRERG
                     ILKGAKRAERVAAEGTIGVYVHHDKKMAVLVEVNSETDFVAKTDEFQELARNLAMQIG
                     ARSPRYIQREDVPEEEIEQERRIHRAQAEQEGKPAQAIDKIVEGRIAKWFEEVCLLEQ
                     PFIRDESRKVKDLIDDVVARLGEKIVVRRFTRYRVGENLG"
     gene            1269..1988
                     /gene="pyrH"
                     /locus_tag="PLY56_16670"
     CDS             1269..1988
                     /gene="pyrH"
                     /locus_tag="PLY56_16670"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ23161.1"
                     /translation="MATMPKWRRVLLKLSGEALAGDGQSSISAEVVHGIALELKEIWE
                     EGIQITLIVGGGNIFRGSQAAQVGMDRATADYAGMLATVINALFLQDALEKVGVDTRV
                     QTAIQMQEVAEPFIRRRAERHLEKGRIVIFAAGTGNPYFTTDTAAALRANEIGAEVLL
                     MAKNRVDGVYDRDPRQDPTARRFRHLGYMEAIQGGYRVMDMTAFSLCMENKMPIVVFD
                     IGVRGNIRRVIYGEEIGTLIS"
BASE COUNT          415 a          583 c          763 g          355 t
ORIGIN      
        1 aactacgtga tcccgggcaa cgacgacgcc attcgtgcta tccgcctgat cacctccaag
       61 atggccgaag cggtcctcga gggccggccc atcaaggaag agtttgtgcc tgcggaagag
      121 gaagtggccc gagccgaagc ggaagaggcc gctgaagggg ccgaagagga agccgctgag
      181 ggtgaggcgt gggagccgga ggagactgtc gctatgggcc ccagcgccga cgagatcgaa
      241 gcgagcttcg ccgcggaggt tgaggaagag ggcggcatcg aggccgagta gcctcgcacc
      301 aggtttcgcg gtgggcatga cgtgtgcccc gcgcgggatg ccgccgcgag ccgaaggcag
      361 ttcgcccggg caaccgttgc gggcgaatgt ttcacgccca aggccggcgt ggaagtatgg
      421 aagggcgacg gaccggcacc agcgggtgcg ttcgccaggt ggtggtaacc gcgctcgccg
      481 tgaagggtga cttccggtga gcgcggttcc ttggcaccgg tgagcgcgcg tcggaacgat
      541 acgcagacgg gtgttctgtt tggatggtcc gggccgtagg gccctctttt tttgggtggc
      601 gattctcccc aggaagggtg aagtggggag agaccgctgt atcattggtg atggagggaa
      661 actctcatgg ctgtaacagc cgcggaagtg cgcgagctgc gtgagcgctc tggcgctggg
      721 atgatggatt gcaagcgcgc cctggaagag acgggtggcg atcttgagaa ggcgatgact
      781 ttgctccgcg agcgcggcat cctgaaaggg gccaagcgcg ccgagcgcgt cgccgccgaa
      841 ggcacaattg gtgtctacgt gcaccacgac aagaagatgg ccgtcctggt ggaggtgaac
      901 agcgagacgg acttcgtcgc gaagacggac gagttccagg agctggcgcg caacctggcg
      961 atgcagatcg gtgcccgctc gccgcgctac atccagcgcg aggacgtgcc ggaggaagag
     1021 atcgagcagg agcgccgcat ccaccgcgct caggccgagc aggaaggaaa gccggcgcag
     1081 gcgatcgata agatcgtcga ggggcgcatc gccaagtggt tcgaggaggt ctgcctcctg
     1141 gagcagccgt tcatccgcga tgagtcgcgg aaggtgaagg acctgatcga tgatgtggta
     1201 gctcgcctgg gcgagaagat cgtcgtccgc cgcttcacgc gctatcgcgt gggagagaac
     1261 ctcggctaat ggctaccatg cccaaatggc gccgggtgct gctaaagctg agtggcgagg
     1321 cgctggccgg agatggccag tcgagcatct ccgcggaggt tgtgcacggg attgcccttg
     1381 aactgaagga gatttgggaa gagggcattc agatcacgct catcgtgggc ggcgggaata
     1441 tcttccgagg atcccaggcg gcccaggtgg gcatggatcg cgcgaccgcc gactatgcgg
     1501 gaatgctcgc aaccgtgatc aacgcgctct tccttcagga tgccctcgag aaggttggtg
     1561 tggatacgcg ggtgcagacg gcgatccaga tgcaggaggt tgccgagccg ttcatccggc
     1621 ggcgcgccga gcggcacctg gagaagggcc gtatcgtcat ctttgctgcc ggcacgggca
     1681 acccctactt cacgacggat accgcggcgg cgctccgcgc caatgagatt ggggctgaag
     1741 tcctcctcat ggcgaagaac cgggtggatg gcgtgtatga ccgcgatccc cggcaagatc
     1801 ccaccgcgcg ccgcttccga cacctgggtt acatggaggc catccaaggc ggctaccgcg
     1861 tgatggatat gaccgcattc tccctttgca tggagaataa gatgccgatt gtggtgtttg
     1921 atatcggcgt gaggggcaac atccgccggg tcatctacgg cgaagagatc ggcacgttga
     1981 tcagctgacc gcctcggttt caggggcatc tcgcaggcgc ggtggcctgc gggatgcccc
     2041 acccatcgac agagcctatc gaattcttgc aggttacccg cctcgaagcg agaagtgtag
     2101 agacataaag aacctg
//
LOCUS       DAOSCV010000823         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34601_length_2113_cov_3.443635, whole genome shotgun sequence.
ACCESSION   DAOSCV010000823 DAOSCV010000000
VERSION     DAOSCV010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34601_length_2113_cov_3.443635"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLY56_16675"
     CDS             complement(<1..580)
                     /locus_tag="PLY56_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23162.1"
                     /translation="MDISVLRRGLGGLGRAARRAGKVCAAGGDQAAHVPLVRDGHTSR
                     CASMACAVAFSLLAGVWSVGAAAAPAAGMAQVSVSVQKLPDKICVRLKAGTPLGRHFL
                     RSVGRAGDVALRIPDCTAKPVRIPVSTGSIRAVSARPTGNDLVVTVERDVAGGYRSAY
                     TANRREIVLEVDMASPAAARELAVPGVARATVP"
     gene            complement(652..882)
                     /locus_tag="PLY56_16680"
     CDS             complement(652..882)
                     /locus_tag="PLY56_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23163.1"
                     /translation="MRGPIAGATPPALQLTGIIDGDPRMAVIEGETAHYIVREGDRVA
                     GGYRVASISPQKVMLRSEDNRTVVLRFGRKGG"
     gene            complement(1562..>2113)
                     /locus_tag="PLY56_16685"
     CDS             complement(1562..>2113)
                     /locus_tag="PLY56_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23164.1"
                     /translation="ARSSGTVPCSPPVAKTTTASKLAPHRFITLNLSLQGSFHNVTRF
                     LYRLTGVSKVISVDGLQVVPAPEGPADHVRVTAQATAYVLKNVPQATAMAAAKLQEIA
                     EAEEKFKAQTQGFGTLEELRAAGLSELKEGETIGGYTFTTDKKTSRSTYKILATSKDP
                     KLHSLSIDKSKKIMDETDKVPYD"
BASE COUNT          317 a          743 c          685 g          368 t
ORIGIN      
        1 tcggaaccgt ggcgcgcgcc actcccggca cggccagctc gcgggccgcg gccggggaag
       61 ccatatcgac ctccagcacg atctcgcgcc ggttggccgt ataggccgag cgatagcccc
      121 ccgccacgtc tcgctcgacc gtcacgacca ggtcattgcc cgtcggacga gcggagacgg
      181 cgcgaatcga tcccgtggac accgggatcc ggaccggctt ggcggtgcag tctggtatcc
      241 gcagcgccac atcgccagcc cggccaacag agcggaggaa atgccggccg agcggggtgc
      301 ccgccttcaa gcgaacgcat atcttgtctg gcagcttctg cacgctgacc gagacctggg
      361 ccatgccggc agcgggcgcg gcggctgccc ccacgctcca cacgccagcc agcaggctga
      421 acgcaacagc gcaggccatg ctggcgcatc gtgacgtgtg gccgtcgcgc acgaggggca
      481 cgtgcgctgc ctggtcaccg ccggcggcgc acaccttgcc cgcgcggcgc gcggcgcgcc
      541 ccagaccgcc taggcctctc ctcagtacgc tgatatccac ggttcgccct ttcccagtct
      601 tggccggcaa ccgggcgcgc cggccagcgt cctcgcatat ggcacggcga ttcacccgcc
      661 cttgcgcccg aatcggagga ccacggtgcg gttatcttcc gaacgtagca tcaccttctg
      721 tggcgagatg gaggcaaccc gatacccgcc cgcgacgcga tccccctcgc gaacgatata
      781 atgcgctgtc tcgccttcaa tgaccgccat gcgcgggtcg ccatcgatga tgccggtgag
      841 ctgcagcgca ggaggagttg cgcccgctat cgggccgcgc accggcgtgg agttcccgat
      901 tctaggcgcc cgcggatcct catgtgacac gagctcaggc gcggggcccg ccagcggtgg
      961 cagcggttcc acgccgagag gctcgccagt gggcttgccc gcttcgcgcc tgcggatggt
     1021 cacctcgggg gctgccttct gggccgcccg cttctctccc ccttccccat ccagccggaa
     1081 cggggaccgc cggggaggcg ggagcggcaa tcgaggcacg ccgggaatgg tatctggatc
     1141 acgtcgcgcg atcacgcgcg aggcgctgga gcctgtgccc gcggcgctgg aagccgactc
     1201 cgcggtggat tggcccggaa agggggactc cactttcgcg gtctcgccgc ccgcctttgg
     1261 cgctccggat ggtgcattgg cgggcgcggc ttgcttggcg ccctgatccg tctttggcgc
     1321 tccggcgggt gtgttggcag gcgcggcttg cttggcgccc tgatccgttt ggccagtctg
     1381 tcccccaggg gcgacggcac ccgccttcgt gccccctccc ggggatgcag gcacgcgcgg
     1441 ctggcaccct ccggcaaggc acaaccacaa gccaagcgcc acggctgtcg ccgctcgcgg
     1501 cttcctgggg gagctgcccg gcagagcccg cgccccaaag accggtgttg actcaaccag
     1561 tctaatcata gggcacctta tccgtctcat ccataatctt cttggacttg tcgatgctca
     1621 ggctgtgcag cttggggtcc ttgctggtgg ccaggatctt gtaggtgctc cggctggtct
     1681 tcttatcggt ggtaaaggta tagcccccga ttgtctcacc ctctttgagc tcgctgagtc
     1741 cggcggctct caactcctca agggtaccaa atccctgggt ctgcgccttg aatttctcct
     1801 cagcctccgc gatctcctgc agcttggcgg ctgccatagc ggttgcctgc ggcacgttct
     1861 tcaggacata ggccgtcgct tgagcagtca cgcgcacgtg atccgctggc ccctcagggg
     1921 ccggcacaac ttgcagccca tccaccgaga tgaccttgga gacacccgtc aggcggtaga
     1981 gaaagcgcgt cacgttgtgg aaactgcctt gtagagacaa gttcagcgtg atgaaacgat
     2041 gcggcgccag tttactcgcc gtcgtggtct tcgccaccgg cggtgagcac ggcaccgtcc
     2101 cggaagacct ggc
//
LOCUS       DAOSCV010000824         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34628_length_2112_cov_3.283422, whole genome shotgun sequence.
ACCESSION   DAOSCV010000824 DAOSCV010000000
VERSION     DAOSCV010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34628_length_2112_cov_3.283422"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1023
                     /locus_tag="PLY56_16690"
     CDS             43..1023
                     /locus_tag="PLY56_16690"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23165.1"
                     /translation="MAVRMGFIGTGGIAQGHMDRLSQIEGVELVSFCDVNLDRARAAA
                     EKFGGRAYSDFAKMLEGEDLTAVFIGTPPFAHGEIELACCERGLHMLIEKPVAIDCDM
                     ARPILKKVKETGVITLVAYKYRWDDHVIKAKEMLAGRTIGLVFGNFWGGLPGAPWWRV
                     QEQSGGQMVEQTTHIVDMARYLAGEIMQVQAFQTRQVMHKKVENCNIADAATANLVFA
                     NGAVGTISNTCMLAGWGQSSCRVMAEGFTLNIAGAVLTWNSAEESGEYTKQEDGYLGE
                     DRAFIHAIKTGDRSVIHSDYEDAYRTLAATVAVNRSAETGGAVISVADLL"
     gene            1149..>2112
                     /locus_tag="PLY56_16695"
     CDS             1149..>2112
                     /locus_tag="PLY56_16695"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ23166.1"
                     /translation="MHRTLLAFILLALTCLPVAGQDASTNLATTEWAIPSAVSSAPEM
                     AYTAPKATDGKPDTGWVSSDEGYPQWLRVEGRFAFEIREVAFRQFPGCPVEGVGGIGR
                     YAVEALQDGQWERLASGDATGKPADAVVRVPLPKPVTTRAIRLVIESGAARRVAVTEF
                     QVLGPKPILPWDYAPAWTGRLIWVQPTLPLPSRQPNRRYFRRAFNLEDPGSVREAWLL
                     ACAFDRLNARWLNNRQVLRDISYNGSMCRRANVVKIPLDLLAQGENVLAASVDDLYDH
                     GSHGLLAELVLLHKDGTRQVIATDSSWKGHPFQGAPPAWRKPGFN"
BASE COUNT          354 a          668 c          753 g          337 t
ORIGIN      
        1 cgggcggcgc gggccatggg aaaaggacag gggaaagaac cgatggcagt ccggatgggc
       61 tttattggca cgggcgggat cgcccagggc cacatggacc ggctctcgca aatcgaaggc
      121 gtcgagctgg tcagcttctg cgacgtcaat ctcgaccgtg cccgcgcggc cgcggagaag
      181 tttggcgggc gagcctacag tgacttcgcg aagatgctgg agggagagga cctgaccgcg
      241 gtcttcatcg gcacgcctcc gttcgcgcac ggcgagatcg aactggcgtg ttgcgagcgc
      301 ggcctccata tgctgattga gaagccggtg gcaatcgatt gcgacatggc ccggccgatc
      361 ctgaagaagg tgaaggagac cggcgtgatc acgctggtcg cctacaagta ccgctgggat
      421 gaccacgtga tcaaggcgaa ggagatgctc gccgggcgca cgattggtct cgtcttcggc
      481 aacttctggg gcgggctgcc gggcgcgccg tggtggcgcg tgcaggagca gtcgggcggg
      541 cagatggtcg agcagacgac gcatatcgtc gacatggcgc gctacctggc gggcgagatc
      601 atgcaggtgc aggcgttcca gactcgccag gtgatgcaca agaaggtgga aaactgcaac
      661 atcgccgacg ccgccaccgc caacctcgtc ttcgccaacg gcgccgtggg caccatcagc
      721 aacacgtgca tgctggcggg atggggccag agctcgtgcc gcgtgatggc cgagggcttc
      781 acgctcaaca tcgccggtgc ggtgctcacc tggaactccg ccgaggagag tggcgagtac
      841 acgaagcagg aggatggcta cctaggcgag gaccgcgcct tcatccatgc catcaagacg
      901 ggcgaccgca gcgtcatcca ttccgattac gaggatgcct atcggacgct cgctgccacc
      961 gtcgcggtta atcgctccgc cgagacgggc ggcgccgtca tcagcgtcgc cgatctcctc
     1021 tgagagggtc tctggcactc tatctctcgg gctgccgggg acggggagcg gctcctgccg
     1081 cctcctgagc ctccggcggc ccctgttcgc gggcgctctt gccgcgccac tccatgcaag
     1141 gctgtctcat gcaccgaaca cttctcgcat tcattctcct ggctctcacc tgcctgcccg
     1201 tcgccgggca ggacgcgagc acgaacctgg cgaccaccga gtgggcgatc ccgagcgcgg
     1261 tctcgtccgc gccggagatg gcctacaccg ccccgaaggc gaccgatggc aagcccgaca
     1321 cgggctgggt ctcctcggat gaaggctacc cccagtggct gcgcgtggag gggcggtttg
     1381 ccttcgagat acgagaggtg gcgttccgcc agttccccgg gtgcccggtg gaaggcgtgg
     1441 gcgggatcgg gcgctacgcg gtagaggcgc tgcaggacgg gcagtgggag cgcctggcga
     1501 gcggcgatgc cacgggcaag ccggccgatg ccgtggtccg cgtgcccttg cccaaaccgg
     1561 tgacaacgcg agcaatccgg ctggtgatcg agagtggcgc cgcccggcgc gtggcggtga
     1621 ccgagttcca ggtgctcggc ccgaagccga ttttgccctg ggactacgcg ccggcgtgga
     1681 cggggcgtct catctgggtg cagcccacgc tcccgctgcc gagccggcag cccaaccggc
     1741 ggtatttccg gcgcgcgttc aatctggagg atccggggag cgtgcgcgag gcgtggcttc
     1801 tcgcctgcgc gttcgaccgg ctgaacgcgc ggtggctgaa caaccggcag gtactgcgcg
     1861 acatcagcta caacgggagc atgtgccggc gcgccaacgt ggtgaagatc ccgctggatc
     1921 tcctggcaca gggcgagaac gtgctcgcgg cgagcgtaga tgacctctat gatcatggct
     1981 cgcacggcct cctcgccgag ctggtgctgt tgcataagga tggcacgcgg caggtgatcg
     2041 cgaccgattc ctcctggaag ggccacccgt tccagggcgc gccgccggcg tggcgtaagc
     2101 cggggttcaa cg
//
LOCUS       DAOSCV010000825         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34631_length_2112_cov_3.059310, whole genome shotgun sequence.
ACCESSION   DAOSCV010000825 DAOSCV010000000
VERSION     DAOSCV010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34631_length_2112_cov_3.059310"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PLY56_16700"
     CDS             complement(<1..1535)
                     /locus_tag="PLY56_16700"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ23167.1"
                     /translation="MKNVFDIREYGASSEPGAINTRAIQSAIDACHAAGGGVVYCPPG
                     TFVTGTLWLKSHVTLYLEAGCVLLGSPRRADYEENLPFPESVAFASEKVSDAHLIVAY
                     QAEQVAITGRGRIDGNSAAFLPFLAQGTNGGARSWEWRPGQMLFFCRCHGVRVEGVEL
                     VNSPYWTLFFHGCEQVTARGIVIANPDDTPNGDGIDIDCCRDVTVDSCQIRSGDDSIT
                     LRGNAKPLGDRPMPCENVTVTNCTLHSACNAIRVGVGSGLVRNCVFSNLVIHDSNVGI
                     NVISRYSSASEGVEIRRVRFDNVVIDARMPLYVSAGVEGQVPVADLAFTGITARGTTI
                     SYIGGTQNNPVSGITLRDVELTLRGGAGNQSFNPEFVGCPSWTLRSQGVPCALAITEA
                     EEVLLQNVRIRWEELDGPWEYGLFARGVRDLALSHVDLSSPPVAGEGAAAIHCVRCSE
                     VLLRGCRAGKGTRRFLSVAKSPEGAGVRLLGNDFANAEAAFASDAPVLEAGNLQADLR
                     RAG"
     gene            1889..>2112
                     /locus_tag="PLY56_16705"
     CDS             1889..>2112
                     /locus_tag="PLY56_16705"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ23168.1"
                     /translation="MRVTVEDVAAGARSVGVQPGDTVMFHSSLSSMGHVVGGPNTVIE
                     GFLRAVGPEGTVAVPTLWWNGTQDLADWDP"
BASE COUNT          345 a          709 c          723 g          335 t
ORIGIN      
        1 atgccggcgc ggcgcaggtc ggcctgcagg ttgccggctt ccagcaccgg cgcatcgctg
       61 gcgaacgcag cctccgcatt tgcgaagtcg ttgcccagca ggcggacgcc tgcgccctcg
      121 gggcttttcg ctacggagag gaaccggcgc gtgcccttgc cagcgcggca gccgcgcaac
      181 aggacctcgg aacagcgcac gcaatggatc gccgccgcgc cctcgcctgc caccgggggc
      241 gaagagaggt ccacgtgaga gagggcgagg tcgcgcacgc ctcgcgcgaa gaggccgtac
      301 tcccacggcc cgtccagctc ctcccagcgg atgcgcacgt tctgaaggag cacctcctca
      361 gcctcggtga tcgcgagcgc gcatggcacg ccttgcgagc ggagcgtcca gcttgggcac
      421 cccacgaact cggggttgaa gctctggttg ccggcgccgc cccgcagcgt cagctccacg
      481 tctcgcaagg tgatgccaga aaccgggttg ttctgggtgc ccccgatgta gctgatggtg
      541 gtgccgcgcg ccgtaatccc ggtgaacgcc agatcggcta ccggtacctg gccttccacg
      601 ccagcggaga cgtagagcgg catccgtgcg tcgatcacga cgttgtcgaa gcgaacgcgc
      661 cggatctcca caccctcgga cgcgctcgag tagcgcgaga tgacgttgat tcccacgttg
      721 gagtcgtgga tgaccaggtt ggagaagacg cagttgcgga cgagccccga gcccacgccc
      781 acgcggatcg cgttgcacgc ggagtgcagc gtgcagttgg tcacggtgac gttctcgcac
      841 ggcatcgggc ggtcgcccag cggtttcgcg ttcccccgca aggtgatgct gtcgtctccc
      901 gagcggatct ggcacgaatc gaccgtcacg tcgcggcagc aatcgatgtc gatcccgtcg
      961 ccattgggcg tgtcgtccgg attggcgatc acaatcccgc gcgccgtcac ctgctcgcac
     1021 ccatggaaga agagcgtcca gtagggggag ttgaccagct ctacgccctc gacgcgcacc
     1081 ccgtggcatc ggcagaagaa gagcatctgc ccggggcgcc actcccagct cctggcgcct
     1141 ccattcgtgc cctgcgccag gaacggcagg aacgccgcgc tgttgccatc gatccgcccg
     1201 cgaccggtga tcgccacctg ctccgcctgg taggcgacta tcaggtgggc atcgctgacc
     1261 ttctcggacg cgaaggcaac cgactccggg aagggcaggt tctcctcata atcggcccgg
     1321 cgcgggctac cgagcaacac gcaccccgcc tccaggtaga gcgtgacgtg gctcttcagc
     1381 cagagcgtgc cggtgacaaa ggtgcctggt gggcagtaga ccacgccccc gcctgcggcg
     1441 tggcaggcat cgatcgcgct ctggatggcg cgcgtgttga tggcgccggg ctccgatgac
     1501 gcgccgtact cccgaatgtc gaaaacgttc ttcatcgctc cactcctcgc ccaggtcgct
     1561 cttgaggggg ccggcaggcc gaaacccgcg cccctcccgt cctgtggttt cggtgcgtgc
     1621 ggtggcgctc ccccctcatc ttccaggtgc gccccagacg cggcgctctc ctcctggcgc
     1681 gataccctca catggccggt accgcgccgg acgtactggc caggggcgcc cgagagaaga
     1741 ggcctccggg gaggctcgca cgaaatgaga ggaaaggagt tgcctccgga agcgcgaacg
     1801 atcttggtcg cactaccccg cgtggcaact gcggggcgcc ctgggaaggg agatggaacg
     1861 gatgaggcct gaaccgggag atccatcggt gagagtcacc gttgaggatg tggctgccgg
     1921 ggcacgcagc gtcggcgtgc agccgggcga tacggtgatg ttccacagct cgttgagcag
     1981 catgggccac gtggtgggtg gcccgaatac ggtgatcgag ggcttcctgc gcgcagtcgg
     2041 gccggagggt accgtggccg tgccgacgct gtggtggaac ggcacgcagg acctggctga
     2101 ttgggacccg ga
//
LOCUS       DAOSCV010000826         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34665_length_2111_cov_3.020914, whole genome shotgun sequence.
ACCESSION   DAOSCV010000826 DAOSCV010000000
VERSION     DAOSCV010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34665_length_2111_cov_3.020914"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLY56_16710"
     CDS             <1..691
                     /locus_tag="PLY56_16710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ23169.1"
                     /translation="GALVVASIPGAFDIPRALMGEEVACLAYYEQPELMEDILATLSD
                     TAFRVLERVSQEVTIDQLSVHEDFAGKSGPLVGPAQIRQYFRPYYQRIWDLLSARGAR
                     IFQQDTDGNINPVIDALLECGLTSILPMEPAAGMDVVELRRKYGRRLAMAGGIDKHVL
                     RQSKEAIRAELEYKMQPLMRQGGYLFGLDHRIPNGTPLENYRYYVDLGREILGLPPRS
                     PESKGWRRMAF"
     gene            807..>2111
                     /locus_tag="PLY56_16715"
     CDS             807..>2111
                     /locus_tag="PLY56_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23170.1"
                     /translation="MARLLAVICLVYCSIAAAAAEPGLPEWTAKIRMDHPRLFFNRDT
                     WPAVRERALGPEKKWYEQYKRRVDKLQEEIGGLADPPRDLGPQAAWAAFVYLMTGERP
                     YLDLAKKALEISLRFYEKCYEERKAVNWYSTTRVHAVMAWDWLYNDLTEAERKEYFSR
                     LVQVLHKVYTAKPPIYRESLSGHTTGFYGVTNCKWFVGLAGLGSGIEEALVREWLVWG
                     YNENMKMLAHRKRACGDDGGSASPTLGYAFGAYPWSEQNFFYTWLSATGENIAPEWPH
                     SAWLANYVLWNWIAAERAPREFGWGDTPHTTNTMPHYELYTHMANIRHLYGKVAPEAA
                     ALARHLQEMLPHKEYAGNWFIYPFLLTDLDKSPEAFHPERLPAARHFESLGQIFMRSG
                     TGPEDTYCLFTAGGILSQHRHYDALSFIIYHRGYLALDSGTRY"
BASE COUNT          382 a          666 c          689 g          374 t
ORIGIN      
        1 gggcgcgctc gtggtcgcga gtatccccgg tgccttcgat atcccccgcg cgttgatggg
       61 cgaagaggtc gcctgcctgg cctactacga gcagccggag ctgatggagg atatcctcgc
      121 cacgctcagc gataccgctt tccgcgtcct ggagcgcgtc agccaggagg tgaccatcga
      181 tcagctctcc gtccacgagg atttcgcggg gaaatccgga ccgctcgttg gcccggcaca
      241 gatccggcag tacttcaggc cgtactacca gcgcatctgg gatctcctct ctgcgcgagg
      301 ggcgcgcatc ttccagcagg ataccgatgg aaatatcaat ccggtcatcg acgcgctgtt
      361 ggagtgcggg ctcaccagca tcttgcccat ggagccagcc gcgggcatgg atgtagtaga
      421 gttgcgccgt aaatacggcc ggcgcctggc gatggccggc ggcatcgata agcacgtctt
      481 gcgccagagc aaagaggcga tccgggcgga gctagagtac aagatgcagc cgctcatgcg
      541 ccagggcggc taccttttcg ggctcgacca ccgcatcccc aacggcacgc ctttggagaa
      601 ctaccgctat tatgtggacc tgggccgcga gatcctcggc ctcccgcccc gctcgccaga
      661 gtccaagggg tggcggcgga tggcgttctg aggaggcacg ccgtcgcggc gagcgaggcg
      721 acgccctcgc gggccaggga gccgggaggg ggatggcgaa cgcatccccg ggcaactgtc
      781 actccccgaa ggaggttggt agcgctatgg cacggttact cgcagtcatc tgtctcgtct
      841 attgctccat cgccgcagca gcggcggagc cgggcctgcc tgagtggacc gcgaagatcc
      901 gaatggacca tccccggctt ttcttcaatc gcgacacctg gcccgccgtg cgcgaacgcg
      961 ccttgggccc tgaaaagaag tggtacgagc agtataagcg ccgggtcgac aagctccagg
     1021 aggagattgg cgggttggcg gatccgccgc gtgacctggg cccgcaggcc gcctgggccg
     1081 cgtttgtcta cctcatgacg ggcgagcggc cctatctcga cctggcgaag aaggcgttgg
     1141 agatcagcct tcgcttctac gaaaagtgct atgaggagcg caaagcggtc aactggtatt
     1201 cgacgacgcg cgtccacgcg gtcatggcgt gggactggct ctacaacgac ctcacggaag
     1261 ccgagcgcaa ggagtatttc tcgcggctgg tgcaggtact ccataaggtg tatactgcca
     1321 aaccacctat ctaccgcgag agcctgagcg gccataccac cgggttctac ggcgtgacga
     1381 actgcaagtg gttcgtgggc ctggcggggc ttggatccgg gatcgaggaa gcgctggttc
     1441 gcgagtggct ggtgtggggc tataacgaga atatgaagat gctggcccat cggaagcgcg
     1501 cgtgcggcga cgatggtggc tcggcgtcgc caaccctggg ctatgccttt ggcgcctacc
     1561 cgtggtcgga gcagaacttc ttctatacct ggctgagcgc caccggcgag aacatcgccc
     1621 cggagtggcc gcacagcgcc tggctcgcca actacgttct ctggaactgg atcgcggcgg
     1681 agcgcgctcc tcgcgagttc ggttgggggg atacaccgca caccaccaac acgatgcccc
     1741 actatgaact ctatacgcac atggcgaaca tccgccatct gtatggcaag gtcgccccgg
     1801 aggcggcggc gctggcgcgg cacctccagg agatgctccc ccacaaggag tacgcgggca
     1861 actggttcat ctaccccttc ctgctgacgg atctggataa gtcgccggag gcgttccatc
     1921 cagagcgcct gccggccgcg cgccacttcg agagcctggg gcagatcttc atgcgctcgg
     1981 gcacggggcc cgaggatacc tactgcctct ttacggccgg cggtattctg tcgcagcacc
     2041 ggcactacga tgccctcagc ttcatcatct accatcgcgg gtatctggcg ctcgatagcg
     2101 gcacgcgcta c
//
LOCUS       DAOSCV010000827         2107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34750_length_2107_cov_2.903021, whole genome shotgun sequence.
ACCESSION   DAOSCV010000827 DAOSCV010000000
VERSION     DAOSCV010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34750_length_2107_cov_2.903021"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PLY56_16720"
     CDS             complement(<1..1391)
                     /locus_tag="PLY56_16720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ23171.1"
                     /translation="MINKARLDHSRRVLRPFQSKGVTWGAATLAPGRGILIADEPGLG
                     KTAQALHITHVAEVGRTLVVTPASLKLNWEREIAKWLPGSRVQVVMTGYDDIRPDTDF
                     TVINYELVWRRYAFLKEQRYQGIIFDEMHYLRGESSMRTTVATELVKPIPYRIGLTGT
                     PIINRPAELVPQLRILGRLWLDDHQFKHRYCGPAQVWNGHRMVWEFRGHSNLDDLHEH
                     LRAFAIRREKAAVAPELPRKQRTVLPVTLGNMAQYRRAEEEYVAWAEKNADALRMARG
                     MAADEDWFLFEEKPPTAGQLGRLQGEQFRHLAKLRRLIAQGKRKLALEWIKSFLPTGQ
                     KLLVFALTIEVQEALRDAFHGCASILGEYSQRKRMEQVDRFQTDPSCQLMVASLKAAA
                     EGLTLTATNNVLVVELPWAPTHLEQAEDRTHRIGQTLPVNVWLLVAAGTLEERILEIL
                     DRKQQVADRAVDGR"
     gene            1827..>2107
                     /locus_tag="PLY56_16725"
     CDS             1827..>2107
                     /locus_tag="PLY56_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOJ23172.1"
                     /translation="MSQRFGRYEIERELGRGASGIVYLASDKALNRQVALKVLTRPQS
                     IQGVERTQALERFVREARAAAAISHPSVPQIYDAGSMAGKCYIAMEYCHG"
BASE COUNT          360 a          650 c          732 g          365 t
ORIGIN      
        1 cggccgtcca cggcgcggtc ggccacctgc tgcttccggt cgaggatctc caggatccgc
       61 tcttccaggg tgcccgcggc gacgaggagc cagacgttca ccgggagcgt ctgcccgatg
      121 cggtgcgtgc ggtcctcggc ctgctccagg tgcgtcggcg cccagggaag ctctaccacc
      181 aggacgttgt tcgtggcggt gagggtgagc ccttcagccg ccgccttcag actggcgacc
      241 atgagctggc agctcgggtc ggtctggaag cggtcgacct gctccatgcg cttccgctgc
      301 gagtactcgc ccaggatgga cgcgcagccg tggaaggcgt cgcgcagggc ttcctgcacc
      361 tcgatggtga gggcgaagac gaggagcttc tgcccggtcg gcaggaagct cttgatccac
      421 tcgagagcga gcttccgctt gccctgggcg atgaggcggc gcagcttcgc caggtggcgg
      481 aactgctcgc cctggagacg cccgagctgc ccggcggtcg gcggcttctc ctcaaagagg
      541 aaccagtcct cgtcggccgc catgccgcgc gccatgcgca gggcgtcggc gttcttctcg
      601 gcccaggcca cgtactcttc ctcggcgcgc cggtactgcg ccatattccc gagggtgacc
      661 gggagaaccg tgcgctgctt gcggggcagc tccggcgcga cggccgcctt ctcgcgccgg
      721 atagcgaagg cgcgcaggtg ctcgtgcagg tcgtccaggt tggagtggcc gcggaactcc
      781 cagaccatgc ggtggccgtt ccacacctgc gccggcccgc agtagcggtg cttgaactgg
      841 tggtcatcga gccagaggcg cccgaggatc cggagctgcg gcaccagctc ggccggccgg
      901 ttgatgatgg gcgtgcccgt gaggccgatg cggtaaggga tcggcttcac caactccgtg
      961 gccacggtgg tgcgcatgct cgactcgccg cgcaggtagt gcatctcgtc gaagatgatg
     1021 ccctggtagc gctgctcctt caggaaggcg tagcggcgcc acaccagctc gtagttgatg
     1081 accgtgaagt cggtgtccgg ccggatgtcg tcgtagccgg tcatcaccac ctggacccgg
     1141 gagccgggca gccacttcgc aatctcgcgc tcccagttca gcttcaggct ggccggcgtg
     1201 acgaccagcg tgcggccgac ttcggcgacg tgggtgatgt gcagggcctg cgcagtcttc
     1261 cccaggccgg gctcgtcggc gatgaggatg ccgcgaccgg gggccagggt ggcggcaccc
     1321 caggtgacgc ctttggactg aaacgggcgg aggacacgac gggagtgatc gagacgggct
     1381 ttgttgatca aacggtctcc tttccttgtt gattataaca cacctgatgc cgcaacgagc
     1441 gcaagcactg gcatccgacc gtagtgacct ggatatgctc cttatccctg atcgacgtcc
     1501 cagttgctcg catagaggag gataccgtcc ggacgcatgg aacaggagca ttgcgttgaa
     1561 ccagatcacc cacacatgga tcaccgatag ggtgtcaggc cggcaccact ccgcagactg
     1621 acgggttcag cctactggtg gcgtgaccgc cgtcccttgg tggaaccatc gtgcatcaca
     1681 gaccgtttgc gcgccggctg agatgacgag accgggctat aggccagtcg acccgttcga
     1741 cgcacgcatg tggatgacat atgggggtga gaacaactct ggcgagttga caccacggcc
     1801 ggccttagcg gaagtggagg ctatggatgt cgcagaggtt cggacgttac gagatcgagc
     1861 gcgagctagg acgtggggca agcggaatag tctacctggc gagcgataag gcgctgaaca
     1921 ggcaggtagc actcaaagtg ctgacgcgcc cccagagcat acagggggta gagcgcacgc
     1981 aagcgctgga gcgcttcgta cgtgaggcac gcgccgcggc agcgattagc cacccgtcgg
     2041 tgccgcagat ctacgacgca ggcagtatgg cagggaagtg ctatatcgcg atggagtatt
     2101 gtcatgg
//
LOCUS       DAOSCV010000828         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34818_length_2104_cov_3.257199, whole genome shotgun sequence.
ACCESSION   DAOSCV010000828 DAOSCV010000000
VERSION     DAOSCV010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34818_length_2104_cov_3.257199"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..444
                     /locus_tag="PLY56_16730"
     CDS             202..444
                     /locus_tag="PLY56_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ23173.1"
                     /translation="MPTYDYYCKACDTHFEVMQRITEPPLTQCQQCGGHIQRLISPVG
                     VIFKGSGFHVNDYRKPEPAKDSESKADSSATSASKS"
     gene            complement(523..>2104)
                     /locus_tag="PLY56_16735"
     CDS             complement(523..>2104)
                     /locus_tag="PLY56_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23174.1"
                     /translation="GTIVTADGSALPEGIEVKLRDASGAVHATATANADGTFRVISHV
                     TGAVISDITDAFELVVLPPAGSGWVPVLVPDLELTNQGGSALLKLSVQLQPQPQLAPG
                     PGLAMITVPFAFRDGAVETVLGDPDAVVYWYDSAAKRYRIAGIDGVPPAAPGRGFWAK
                     PRRGGTPYEIAAPAILPPQDQPFMLPLAKGWQIIGSPYITGSIPLSQVKVLLPGSVTP
                     VPLGDPATINLVRSYLWTYNGSTYDLVSATPEGKTAAIAPYQGYWIRALVDGVWLVFS
                     PPSASEPPMEARQRSASAGWRARVEARVAGRAHSACVFGVGPTAYTIDAPPPPSGHAE
                     VRLTTPGGERAAVAVLEEGTPASWSFEVDTAGVPEGEEVTVAWPGLGTVPRDVRLVLV
                     DEQTGRRLQMRTTACVTFRARTAPYRFRIEAQKATGALISISGITLTPVRGAGRSIGY
                     VASTDATVSITIKSPSGQTLSRVVTGRAVSQGRNEAYWDGRDFRGVPVKGMVLVEITA
                     TTGEGATVRAVRPAMLVD"
BASE COUNT          357 a          734 c          666 g          347 t
ORIGIN      
        1 agtatcccca acccagcccg gcagggcttc gctcgcaagc agcgcggtga tttaccgccg
       61 cgcgccgggt tgcgcgctgc ctccgaggct tcggattccc tacctcggaa atcgggccga
      121 ttagcactcg ccttgccaga gtgccaacgg ttgtgctata ctacgggtga gatattctcc
      181 aattcagagg ggtggtgtgt cgtgccaacc tacgactact attgcaaagc gtgtgatacc
      241 catttcgagg tgatgcagcg cattaccgag ccgccgctga cccagtgcca gcagtgtggc
      301 ggtcacattc agcgcctgat ctctccggtt ggagtcatct tcaagggctc tggattccac
      361 gtcaacgact accggaagcc cgaaccggcg aaagactccg agagcaaggc ggattcctca
      421 gccacatcgg catccaagtc gtaaacgctt gccgaatcgc gcgcgcttta atgaaggggg
      481 atgggcagat gcccatcccc cttctgttgt ttcgcccggc cgtcagtcga ccagcatcgc
      541 cgggcgcacg gcacgcaccg tggcgccctc gcccgtcgtc gcggtgatct ccaccaggac
      601 catgcctttc actggaacgc cccggaagtc gcggccatcc cagtaggcct cgttccggcc
      661 ctggctcacc gcacgcccgg tcaccacacg cgagagcgtc tggcccgagg gcgacttgat
      721 ggtgatggag acggtcgcat cggtgctggc cacgtagccg atgctcctgc ctgccccgcg
      781 caccggggtg agcgtgatcc cggaaatgga gatcagcgcg cccgttgcct tctgcgcctc
      841 gatccggaag cgatacggcg cggtgcgggc ccggaaggtc acgcaggccg tcgtccgcat
      901 ttgcaggcgc cgcccggtct gctcatccac caggaccagg cgcacgtctc gtggcaccgt
      961 cccaaggccg ggccatgcca cggtgacttc ctcgccctcc ggcacgcccg cggtatccac
     1021 ctcaaagctc cagctcgccg gcgtgccctc ttcgagcacc gcgactgcgg cgcgctcgcc
     1081 accaggcgtg gtcaagcgca cctcggcgtg cccgctcggc ggaggcggcg catcgatggt
     1141 gtaggccgtg gggcccaccc cgaatacgca cgcgctgtgc gcgcgcccgg ccacccgagc
     1201 ctcaacccgc gcccgccagc ctgcggaagc cgagcgctgc cgggcttcca tcggcggctc
     1261 gctcgcggac ggaggcgaga agacgagcca cactccatcc accagcgcac ggatccagta
     1321 gccctggtaa ggcgcgatgg cggccgtctt tccttcgggc gtcgccgata cgagatcgta
     1381 cgtcgagccg ttgtaggtcc acaggtagct gcggaccagg ttaatcgtcg ccgggtcacc
     1441 gagcggcact ggcgtcacag aacccgggag gagcaccttc acctgcgaga gcgggatgct
     1501 cccggtgata taggggctgc cgataatctg ccatcccttt gcgagtggca gcatgaaggg
     1561 ctgatcctgc ggcggcagga tggcaggcgc ggcaatctca tacggcgtgc cgccacggcg
     1621 cggtttcgcc cagaagccgc ggcccggggc cgccgggggc acgccatcga tgccggcgat
     1681 ccggtaacgc ttcgccgcgg agtcatacca gtagacgacc gcgtccggat ctccgaggac
     1741 cgtctccacc gcgccgtcgc ggaaggcgaa cggcaccgtg atcatcgcca ggccgggtcc
     1801 aggcgcaagt tgcggctgtg gctgcagctg caccgatagc ttcagaagcg ctgatccacc
     1861 ctggtttgtc agttcgagat ccggaaccag caccggaacc catccggatc cggcgggcgg
     1921 caagaccacg agttcaaagg cgtccgttat gtcgctgatc acggcacccg ttacgtggct
     1981 gatcacacgg aaggtcccat cggcattggc cgtggccgtc gcgtgcacag cgccactggc
     2041 gtcgcgcagc ttcacctcaa tgccctccgg tagcgccgag ccatccgcag tgacaatcgt
     2101 gccc
//
LOCUS       DAOSCV010000829         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34978_length_2097_cov_4.109696, whole genome shotgun sequence.
ACCESSION   DAOSCV010000829 DAOSCV010000000
VERSION     DAOSCV010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34978_length_2097_cov_4.109696"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2097)
                     /locus_tag="PLY56_16740"
     CDS             complement(<1..>2097)
                     /locus_tag="PLY56_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ23175.1"
                     /translation="KWNLWSTDKDAHKKWSGGVVLQSPVVKQDRATPEEGALPLHTRI
                     TGIPAGTYAVELLHLSRPLAVSLDGKNWTRKEGRDNRVGIFTITNGTFELWVDDRYAA
                     TPPGSSYYDALVFTPAVPEKLGVVNPDFEHGAESLTGWRLWTREENAGSVAIAPEGHS
                     GKRAARIRHEGERDWAFTNDGRLNVRPDQLFTVSAWVKGSGHVEIAVVALSGGKTLRW
                     TIGGDGRLATPQWTKLEGEAIVPEECDQIQVRFVGSGKADLLVDNVELREGGRPKVSK
                     PKVNGFAKARIEERLRRGVVAVPLQGGGVYVGWRLLKSDPANVAFNVYRRTGRMPAVR
                     LNQAPITRTTDFVDPQPPAGEQAYYVRPVIDGREGPGSEVATVVPTAEGRAYLSIKLD
                     GTHTFQKVGIADLDGDGRLDFVIKQPNTNVDPYEKYWKRSTDTYKLEAYRSDGTFLWR
                     YDLGWAIECGIWYSPYVVFDFDGDGRAEVAVKTGEGDPRDPDGRVTSGPEYLSILDGR
                     TGKERTRVEWPSREGFPNYNYASRNQLGVAYLDGKTPCLIVERGTYNTIKVVAYEYRG
                     GKLHELWSWNDRESGARYRGQGAHILRAADVDGDGRDEVILGSAVLDDDGTGLWSTGL
                     GHPDNLTVGDLDPERPGLEIQYGIETRNPKNSICMVDAKTGKILWGLQEPSTHIHGSG
                     LCADIDPRYPGVESYQG"
BASE COUNT          340 a          700 c          664 g          393 t
ORIGIN      
        1 ccttggtagc tctccacgcc ggggtagcga ggatcaatgt cggcgcacag cccagacccg
       61 tgaatgtggg tcgaaggctc ctgaaggccc cagaggatct tgccggtctt tgcatccacc
      121 atgcagatgg agttcttcgg gttgcgggtt tcgatgccgt actggatctc caggcccggg
      181 cgctcgggat ccaggtcgcc cacggtgagg ttatcggggt ggcccagtcc ggtggaccag
      241 agcccggtac cgtcatcatc gaggaccgcg gagccaagga tcacctcgtc gcggccatcg
      301 ccatccacat cagcagcgcg gaggatatgc gcgccctggc cgcgatagcg cgcgccactc
      361 tcgcggtcat tccagctcca cagctcgtgc agcttgccgc cgcgatactc ataggcgacc
      421 actttgatcg tgttgtaggt gccgcgctcc acgatgaggc atggtgtttt gccatccaga
      481 taggcgactc cgagctggtt gcggctcgcg taattgtagt tcgggaagcc ctcgcgcgag
      541 ggccactcga cccgggttcg ctccttaccc gtccgcccat ccagaatgga gaggtactcc
      601 gggccgctgg tgacgcgccc gtccggatca cgcgggtcgc cctcgccggt cttgactgcg
      661 acttcggccc gaccatcgcc atcgaagtcg aaaacgacat agggagagta ccagatgccg
      721 cactcaatcg cccacccgag gtcatagcgc cacaggaagg tgccatcgct gcggtaggct
      781 tcgagcttgt aggtgtccgt gctccgcttc cagtacttct cgtagggatc gacgttggta
      841 ttcggctgct tgatcacgaa atcgaggcgg ccgtcgccat ccagatcggc gatgcccacc
      901 ttctggaacg tgtgcgtccc atcgagcttg atggagagat aggcgcggcc ttccgccgtg
      961 gggacgacgg tggccacttc cgagccaggc ccttccctgc catcgatgac agggcggaca
     1021 tagtaggcct gctctcctgc ggggggctga ggatccacga aatcggtcgt ccgggtgatg
     1081 ggcgcctggt tcaatcgcac tgccggcatt cgtccggtcc gccggtagac gttgaacgcc
     1141 acgttggcag ggtcactctt cagcaggcgc catccgacat agacgcctcc gccctgcagt
     1201 ggaacagcaa cgacaccgcg ccgcagccgc tcctcgatgc gcgccttcgc gaagccgttc
     1261 accttcggct ttgacacctt cggccggccc ccctcgcgca gctcgacgtt gtccacgagg
     1321 aggtctgcct ttccagagcc gacgaagcgc acctgaatct ggtcacactc ctcgggaacg
     1381 atagcctcac cctccagctt ggtccactgg ggcgtggcta gccgaccatc gccgccgatc
     1441 gtccaccgca gcgtcttgcc gccagagagg gcgacgacgg ctatctccac gtgcccggag
     1501 cctttcaccc aagcgctgac cgtgaagagt tggtcgggcc gaacgttcag gcggccgtcg
     1561 ttcgtgaacg cccagtcgcg ctcgccctca tgccggattc gggccgcgcg cttgccagag
     1621 tggccctccg gggcaatggc cacagagcca gcgttctcct cgcgcgtcca aaggcgccag
     1681 ccggtcaggc tctcggcgcc atgctcgaag tcggggttca ccactccgag cttctccggc
     1741 accgccggcg taaagacgag ggcatcgtag tagctggatc cgggcggcgt cgctgcgtag
     1801 cggtcatcga cccacagctc gaaggtgccg ttggtgatcg tgaagatccc cacccggttg
     1861 tcccggcctt ccttgcgggt ccagttcttg ccatcgagcg agaccgcgag cggccggctg
     1921 aggtggagga gttcgacggc gtaggtgccc gcgggaatcc ccgtgatgcg cgtatgcagg
     1981 ggcagcgcgc cctcttcggg cgtggcgcga tcctgcttca cgacgggcga ttggaggaca
     2041 acgccaccgg accacttctt gtgggcgtcc ttatccgtgc tccagaggtt ccatttc
//
LOCUS       DAOSCV010000830         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_34987_length_2097_cov_2.779138, whole genome shotgun sequence.
ACCESSION   DAOSCV010000830 DAOSCV010000000
VERSION     DAOSCV010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34987_length_2097_cov_2.779138"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLY56_16745"
     CDS             complement(<1..1166)
                     /locus_tag="PLY56_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23176.1"
                     /translation="MADSKLELVVTVDADKANASIKSVNAGLSSIEATAINAARGASR
                     GIDGMTAAMVKGATAGNLLADAIKTALSWAKAWTIDAAKLAAHEARLEASTLALARAH
                     GVSEAAALKAVEAIRKIGYHGEEALHTITRFIAADMDLAKVEGLAKLAKDAAAIENIA
                     AGEALEKLLMAVEGGYSRGLRTMGLFVNLEKELALEELRRNRALTEAEQKQVRYNAIM
                     REGAKIQGAHAAASGEAEMMLKALGRQMQKLREQVGARFQKEFKDLIKLFKDAAQWAT
                     ENADTLAKFGRVVIEVATVLATYQLAKKIAELAGALSALSLASVGLRAGLIGAGIAAL
                     GFVFYEEKRKWDQRAALLDEEIKRARVQQMVRDGRSLKDLEEAGFSREEITYGMT"
     gene            complement(1159..1413)
                     /locus_tag="PLY56_16750"
     CDS             complement(1159..1413)
                     /locus_tag="PLY56_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23177.1"
                     /translation="MCDPALCPDAPEGGRCDHCPLDRLDAAQSSEPGQLLRRALDLRA
                     AFKLGVKLSLDEIAADEFQAMLIVEEEQARFDEERLNRHG"
     gene            complement(1479..>2097)
                     /locus_tag="PLY56_16755"
     CDS             complement(1479..>2097)
                     /locus_tag="PLY56_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23178.1"
                     /translation="VMEQSNVFDSARQLEIQLRSAEGASTVKVRFPTDEEWIERQRRR
                     KIIIKQLGRGVSETTIPNAEEVDADLFRKIRLDEGDEVDAYEASRIIEQLSLAEVDDV
                     VPEGAAFRVLLRVPGGTTAHVLRMPSAKDVIEYRRSFARILDLPFNRQELTVNLGAAG
                     TLYQKLVQATEGYAGAAPIIHQAVAVKAAIDALDAGFQEERNPNS"
BASE COUNT          314 a          718 c          675 g          390 t
ORIGIN      
        1 gtcatcccgt aggtgatctc ttcgcggctg aagcccgcct cctcgagatc cttgagcgag
       61 cggccgtcgc ggaccatctg ttggacgcgg gcgcgtttga tctcctcgtc gagcaaggcc
      121 gcgcgctggt cccacttgcg cttctcctcg tagaacacga agcccagcgc ggcgatcccg
      181 gccccgatga gaccggcccg cagacccacc gaggccaggc tcaaggcgct caaggccccg
      241 gccagctcgg cgatcttctt cgccaactgg taggtggcca gaaccgtggc cacctcgatg
      301 accacgcgcc cgaacttcgc cagtgtgtcg gcgttctcgg tggcccattg ggcggcgtct
      361 ttgaacagct tgatgaggtc cttgaactcc ttctggaagc gcgcgcccac ctgctcgcgg
      421 agcttctgca tctggcggcc gagcgctttg agcatcatct cggcctcgcc ggaggcggcc
      481 gcgtgcgcgc cctggatctt ggcgccctcg cgcatgatgg cgttgtagcg gacctgcttc
      541 tgctccgctt cggtcagcgc ccggttgcgg cgcagctcct ccagggcaag ctccttctcc
      601 aggttcacga acaggcccat ggtgcgcagg ccgcgcgagt agccgccctc cacggccatg
      661 agcagtttct ccagcgcctc gccggcggcg atgttctcga tggcggcggc gtccttggcg
      721 agcttcgcca gtccctcgac cttggcgagg tccatgtcgg cggcgatgaa ccgggtgatg
      781 gtatgcagtg cctcttcgcc gtggtagccg atcttgcgga tggcttccac agctttcagg
      841 gccgcggctt cgctcacgcc atgcgcgcga gccagagcga gcgtcgaggc ctccaggcgc
      901 gcctcgtgtg ccgccagctt tgccgcgtcg atggtccagg ccttcgccca actcagagcc
      961 gtcttgatgg catcggcgag caggttgccg gcggtggcgc ccttgaccat ggcggcggtc
     1021 atgccgtcga tgccgcgcga ggcgccgcgg gccgcgttga tggcggtcgc ttcgatcgac
     1081 gacaaaccgg cattgacgct cttgatggac gcatttgctt tatccgcatc aacggtgaca
     1141 accagttcga gcttgctgtc agccatggcg gttgagacgc tcctcgtcga agcgcgcctg
     1201 ctcctcttcg acgatcagca tggcctgaaa ctcatccgcg gcgatctcat caagcgacag
     1261 cttgacaccc agcttgaaag ccgcccggag atcgagtgcc cggcgcagca gttggccggg
     1321 ctcggaagac tgcgctgcat ccaaccgatc cagcgggcag tgatcgcagc ggccaccctc
     1381 gggggcgtca gggcagagcg ccggatcaca caactgattg cgccgcagcg accagtagac
     1441 caggtagcgc agggaggggc gctcgggcca ctccccgctc aggagtttgg gttgcgctcc
     1501 tcctggaatc cggcgtccag tgcgtcgatg gcggccttga ccgccaccgc ctggtggatg
     1561 atcggggccg cgccggcgta gccctcggtc gcctgaacga gcttttggta tagcgtgcca
     1621 gcggcaccga gattcacggt cagctcttgg cggttgaacg gcaggtccag gatgcgcgcg
     1681 aagctacggc ggtactcgat cacgtccttg gcgctcggca tgcgcaggac atgtgccgta
     1741 gtcccgccgg gcacgcgcag cagaacccgg aacgcggcgc cctccgggac gacgtcatcc
     1801 acctcggcca ggctcaactg ctcgatgatc cggctggctt cataggcgtc aacttcatcg
     1861 ccctcgtcga ggcggatctt gcggaacagg tccgcatcca cctcctcggc gttggggatg
     1921 gtcgtctccg acaccccacg cccgagctgc ttgatgatga tcttgcggcg ccgctgccgc
     1981 tcgatccact cctcatcggt cgggaagcgg accttcaccg tgctggcgcc ctccgccgag
     2041 cgaagctgga tctcgagctg gcgcgcggaa tcgaacacgt tgctctgttc cataact
//
LOCUS       DAOSCV010000831         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35136_length_2091_cov_2.615914, whole genome shotgun sequence.
ACCESSION   DAOSCV010000831 DAOSCV010000000
VERSION     DAOSCV010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35136_length_2091_cov_2.615914"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLY56_16760"
     CDS             <1..359
                     /locus_tag="PLY56_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23179.1"
                     /translation="ARFDPDETEFKLYRVLDGGRQVGTIMTHLAKGQYGAIEVVVAVD
                     NQGVVKGVGIQRDRERARKALRSEAFLGQFRGKRVSDSFEVGKAIQPAAKGAEVASRA
                     VAFSVKKLLIILDELG"
     gene            387..1421
                     /locus_tag="PLY56_16765"
     CDS             387..1421
                     /locus_tag="PLY56_16765"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOJ23180.1"
                     /translation="MKRMLRIAFVGSLGLILAAPGIRAAEESPSAAPEEPEAAPARSY
                     SIRYRLDVHGMVEKDHPATLEVMAAEAEVTGEVGQHFSYAVQIPFYTSMEGRGRGMHF
                     GNLYVIYRGKLGEPTVKVGQFVLPFGNLPYYETHTRPVQTLYRYSLGARIDPGIAVEG
                     YLGKETEFQVAVTTGNGPYRKDNNGNKVVTARVSHTFELGDNELRLGVSALRGKLPVF
                     SLMSEPGMSGGDVLEFSDKNRVAVDAEFYWGPYLVRAEVVAGDDNGRGAYGHWLQFSY
                     PLSYRTSLEAGMERWSQFSGGLTGYWAGIEHKLSEGKTARLVFETGRRRESGMSMDTA
                     RITGQFLVEF"
     gene            1493..1876
                     /locus_tag="PLY56_16770"
     CDS             1493..1876
                     /locus_tag="PLY56_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23181.1"
                     /translation="MKRIAIVVGVVVVLAGLIAYVAKPGGNRELGQGVPEGAVVTTVG
                     ELAANPGSFEGKEVTVTGKLVQVCPTSGCWGVVDDGTGTVRWDSAPSGWALPPGQSGK
                     EITVHGRVSVNEAGAPQITALGARL"
BASE COUNT          363 a          620 c          781 g          327 t
ORIGIN      
        1 gagcccgctt cgaccccgac gaaaccgagt tcaagctgta tcgcgtgctc gatggcggca
       61 ggcaggtggg cacgatcatg acgcacctgg ccaagggcca gtatggcgcg attgaagtgg
      121 tcgtcgcggt cgataaccag ggcgtggtga agggtgttgg catccagcgc gaccgcgagc
      181 gtgcgcgcaa ggccctccgc agcgaggcgt tcctcggcca gttccggggc aagagagtga
      241 gcgactcgtt tgaggtgggc aaggcgatcc agccggcggc caagggcgcg gaggttgcct
      301 cgcgcgccgt cgccttctcc gtgaagaagc tgctcatcat cctggacgag ctcggctgag
      361 ccacgtgatt cggaggagca acgccgatga agaggatgct tcgtatcgcg ttcgtgggca
      421 gcctggggct catactggcg gccccgggca tccgcgccgc ggaggagagc ccatccgccg
      481 cgccggagga gccggaagcc gcgccggcgc gttcctactc gatccggtat cgcctggacg
      541 tccacggcat ggtggaaaag gaccatcccg cgacgctcga ggtgatggcg gccgaggcag
      601 aggtgacggg cgaggttggc cagcacttct cctatgcggt gcagatcccg ttctatactt
      661 cgatggaggg gcgtggccgg gggatgcact tcggcaacct ctacgtcatc taccggggga
      721 agctgggcga gccgaccgtg aaggtcgggc agtttgtgct tcccttcgga aacctgccct
      781 actacgagac gcacacccgg ccggtccaga cgctctaccg gtacagcctg ggagcccgga
      841 tcgacccggg aatcgccgtg gagggctatc tggggaagga gacggagttt caggtggccg
      901 ttaccacggg caacggcccc taccggaagg acaacaacgg caacaaggtg gtcaccgcgc
      961 gcgtgagcca caccttcgag ctcggcgata acgagctgcg cctgggcgtc tccgcgctcc
     1021 ggggaaagct acccgtcttt tcgctgatga gcgagccggg catgagcggt ggcgacgtcc
     1081 tggagttcag cgacaagaac cgcgtggccg tggacgcgga gttctactgg ggcccctact
     1141 tggtgcgcgc ggaggtggtg gccggcgacg ataacggccg gggcgcctat gggcactggc
     1201 tacagttcag ctatcccctg agctaccgga cctcgctgga agcgggcatg gagcgctgga
     1261 gccaattctc cggcgggctg accggctatt gggcaggcat cgagcacaag ctctctgaag
     1321 gaaagaccgc gcgcctcgtg tttgagacgg ggcggcggcg cgaaagcggc atgtcgatgg
     1381 atactgcgcg gatcaccggg cagtttctgg tggagttcta aggagcagcc cagggttctg
     1441 gggcccaaga cagagtctgc cggccccggg cgctagacga gaggagaagc cgatgaagag
     1501 gatcgcgatc gtcgtgggag tcgtcgtggt gctggccggg ttgatcgcct atgtggcgaa
     1561 gccaggcggg aaccgcgagt tgggccaggg agtgccagag ggcgcggtgg tgaccaccgt
     1621 cggggagctc gcggccaacc cgggctcgtt cgagggcaag gaggtgaccg tgaccggcaa
     1681 gctcgtccag gtgtgcccca cgagtggctg ctggggcgtg gtggacgacg gcaccggcac
     1741 ggtgcgctgg gatagcgcgc cctcgggatg ggcgttgccc cccgggcagt ccggcaagga
     1801 gatcacggta catggccggg tgagcgtgaa cgaagcgggc gctccgcaaa tcacggcgct
     1861 cggcgcgcgg ctctaagcaa gaaggggagc gctgggcacc cgcgggcatc tgcccgaccg
     1921 gcgttccccc tccaccgagc ccgctcttga gagagggtca gggaatgcgg ttcattcact
     1981 tcgcgctgag gaacctgacg cagcggaagg tgcgcaccgg gctgacggtg ttcaccatcg
     2041 cggtggcggt cgcggtgccg atgttggcgt tcatcgcgcc cgaaagccgg c
//
LOCUS       DAOSCV010000832         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35224_length_2087_cov_3.222933, whole genome shotgun sequence.
ACCESSION   DAOSCV010000832 DAOSCV010000000
VERSION     DAOSCV010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35224_length_2087_cov_3.222933"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLY56_16775"
     CDS             complement(<1..240)
                     /locus_tag="PLY56_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23182.1"
                     /translation="MEAYDVVVIGGGPGGYVAAIRAAQLGLRTAVVEREALGGVCLNW
                     GCIPTKALIHNAGLVNTLKQAKEFGIAFDGLRVDYA"
     gene            467..1279
                     /locus_tag="PLY56_16780"
     CDS             467..1279
                     /locus_tag="PLY56_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ23183.1"
                     /translation="MKFIIFTKHLEGRDIPGLIADIRFAGAEGADLCVRPGYPVNPEN
                     APTALPAAMKQFRAEGLDIPLVTTPGDFTDPTAAYAEPLWAACAEAGVRLVKLGYWYM
                     GPDGYWATVDRVRKALEGFARLAEKYGPKACVHTHSGSTMGLNASSVMNLVRGFDPRH
                     IGVFADPGHLSIVGEPLPMALSILGEYLSILAFKDLVRERVVREGKPGWQTKVVPLGT
                     GFVDWTTLLRLLGEIGYDGPISFHSEYSGYPVDTVVDLARIDIRFIRGLMAA"
     gene            1296..>2087
                     /locus_tag="PLY56_16785"
     CDS             1296..>2087
                     /locus_tag="PLY56_16785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ23184.1"
                     /translation="MNILVIAIDTLRADHLGCYGYPLPTSPHIDRLAREGVVFQCFSP
                     HIPTHPGYTTLFTGKDVMAHQIVCQGGKVELDPKVRLLAEILRENGYFTAAADNLGRW
                     FPRGFDLYEGYQWDQDPKGAWRKGEAVNKTAQRILDECVRQPKPWFAFLHYWDPHTPY
                     LPPVPFDRMFYHGNERAPENHSMDAVFAFEPFRYYFQQWLGGVTDINFPKAQYDAEIA
                     YADACLAHVFTRLAGHGVLEDTLVLITADHGEEMDEHEMWFDHHGL"
BASE COUNT          377 a          716 c          617 g          377 t
ORIGIN      
        1 cgcgtaatcc acccgcaggc catcgaaggc aattccaaac tccttcgcct gcttcagtgt
       61 attcaccaac ccggcgttgt ggatgagcgc cttcgtgggg atgcaccccc agttcagaca
      121 gacgccccct agcgcctcgc gctctaccac cgccgtccgc aacccaagct gggcggcgcg
      181 gatggcagcc acatagccgc ccggtccacc accaataacc actacatcat atgcttccat
      241 agcgttcctc aacccccaat cgcggggaaa ccctgcacgc ctgtacttct ggcgctattc
      301 ctcattctcc ggaattataa cagaaacgga gaggcacggc aacgtcgtgt cgcgcggcct
      361 gcgtgagccc gctggtgagg caggagaacc ggcactcccg tcacgaatct gaccacaggc
      421 ggtcgcgctt gttgggcttg cggctgccgg gtgggaggat accaagatga agttcatcat
      481 cttcaccaag catctggagg gcagagacat ccccgggctc attgccgaca ttcgcttcgc
      541 cggggccgaa ggcgccgatc tgtgcgtgcg gcccggctac ccggtgaacc cggagaacgc
      601 gcctacggcg ctgccggccg ccatgaaaca gttccgcgcc gagggactgg atatcccgct
      661 cgtcaccacg ccaggcgatt tcaccgatcc cactgccgcc tacgcggagc cgctatgggc
      721 cgcctgcgcc gaggccggcg tccgcctcgt gaagctcggc tactggtaca tgggcccaga
      781 tggctactgg gccacggtgg atcgggtgcg caaggccctg gagggctttg cccggctggc
      841 cgagaagtat ggcccgaaag cctgcgtgca cactcactcc ggcagcacca tgggcctcaa
      901 cgcctcctcc gtgatgaacc tcgttcgcgg cttcgatccg cggcacatcg gggttttcgc
      961 cgatccgggg catctctcca tagtgggtga gccgttgccg atggccctct ccatcttggg
     1021 agagtacctc tcgatcctcg cattcaagga tctcgtgcgc gagcgcgtcg tgcgcgaagg
     1081 caagcccggg tggcaaacga aggtggttcc cctcgggacc ggcttcgtgg attggaccac
     1141 tctgctgcgc ctcttgggcg agatcggcta cgacggcccg atcagcttcc acagcgagta
     1201 ttccggatac ccggtggaca ccgtcgtgga tctggcccgg atcgatatcc ggtttatccg
     1261 tggcctgatg gccgcgtagg atggacagcg accgcatgaa catcctcgtg atcgcgattg
     1321 atacgcttcg cgcggatcat ctgggctgct atggctaccc tctgcccacc agcccgcata
     1381 tcgaccgatt ggcccgcgaa ggcgtcgtct tccagtgctt cagcccgcac atccctacgc
     1441 acccgggcta taccacgctc ttcaccggca aggacgtgat ggcacaccag atcgtctgcc
     1501 agggcggcaa ggtggagctc gaccccaagg tgcgcctgct cgccgagatt ctccgggaga
     1561 acggctactt caccgcggcg gccgataacc tggggcgttg gttcccgcgc ggctttgacc
     1621 tctatgaggg ataccagtgg gatcaggacc ccaaaggcgc ctggcgcaag ggcgaggcgg
     1681 tgaacaagac cgcacaacgc atcctcgatg agtgcgtccg ccagcctaag ccgtggttcg
     1741 ccttcctcca ctactgggat ccgcacacgc cctacctgcc gcccgtgccg ttcgaccgga
     1801 tgttctatca cggcaacgaa cgcgcccccg agaaccacag catggacgcc gtcttcgcct
     1861 tcgagccgtt ccgctactac ttccagcagt ggctgggcgg cgttaccgac atcaatttcc
     1921 cgaaggcgca atatgacgcc gagatcgcct atgccgacgc ctgcttggcc cacgtcttca
     1981 cccgcctggc cggccatgga gtgctggaag acacgctggt cctcatcacc gccgaccacg
     2041 gcgaggagat ggacgagcac gagatgtggt ttgaccacca cggcctc
//
LOCUS       DAOSCV010000833         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35228_length_2087_cov_3.023130, whole genome shotgun sequence.
ACCESSION   DAOSCV010000833 DAOSCV010000000
VERSION     DAOSCV010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35228_length_2087_cov_3.023130"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="PLY56_16790"
     CDS             <1..584
                     /locus_tag="PLY56_16790"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HOJ23185.1"
                     /translation="VATGRVRTFGPLECCFGYRNSVFKQALRGQYIVLAVRFRLTTRD
                     HRVVTSYGALQEALRARGIEHPTPRDIADAVTEIRRQKLPDPAELGNAGSFFKNPEVS
                     QEQYEALKREHPGLVAFPTPGGAKIAAGWLIEQCGWKGKRVGNTGAHARQALVLVNYG
                     GATGREIYALAQEIQASVLARFGISLEMEVNLV"
     gene            complement(1128..>2087)
                     /locus_tag="PLY56_16795"
     CDS             complement(1128..>2087)
                     /locus_tag="PLY56_16795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HOJ23186.1"
                     /translation="LAMDTTAGERERQSLEPLLATPAARGALVSGKMLAAVLLGLASM
                     LLEFSGIESGGINLKGSSSTGKTTALAAAASLFGGPSYVNRWRATSNGLESLAALHND
                     TLLILDELAQVDPREAGEIALISAERMKHLIDALLDISRMEGDRMPIHLADLNPCQLA
                     DGALNQVSLWARNARVSLARDYHPDAKPVRADRALAERVLVNLLGNALRHSPPETVIT
                     LRVANVAGGVAFSISDQGPGIPAEWLSRIFDKFTQVAARKSGAAVGTGLGLTFCRHAV
                     EAQGGRIGIESQEGKGTTVTVTFPAAPASQADPMEGALMAGGR"
BASE COUNT          337 a          689 c          731 g          330 t
ORIGIN      
        1 aagtggcgac gggccgggtg cgcaccttcg gccccttgga gtgctgcttt ggctaccgga
       61 acagcgtctt caagcaggcg ctgcgtgggc agtacatcgt gctcgcggtg cgcttcaggc
      121 tgacgacgcg cgaccaccgc gtggtcacca gctatggcgc gcttcaggag gcgctacgcg
      181 cgcgcggcat cgagcacccc acgccccgcg acattgccga cgccgtgacc gaaatccgcc
      241 gccagaagct gcccgacccg gcggagctgg ggaacgccgg gagcttcttc aagaaccccg
      301 aggtgagcca ggagcagtat gaggcgctga agcgggagca tccgggcctg gtggcgtttc
      361 ccacgccggg cggagccaaa atcgccgccg gctggctcat cgagcagtgt ggctggaagg
      421 gaaagcgcgt gggcaacacc ggcgcgcacg cccggcaggc gcttgtcctc gtgaactacg
      481 gcggcgcgac cggacgcgag atctatgccc ttgcccagga gatccaggcg tccgtcctcg
      541 cccgcttcgg catctcgctc gagatggaag tcaacctcgt gtgaccccgc tcttgggctt
      601 ccgagataag gaatccgaac ccgggcaggg agactcctgg ctcggtttcc agacctctcc
      661 ggcctctcct tctgccgtca tccgcggtat cggtgtcggg gtgcaacccg gcgagagggg
      721 ctagcccccc gcgctgcttg agagcgaagc cctagcgagc tgggccagac tacgttcccg
      781 ggcaatcagt gagggcggga gctgggaggg ggagcccgaa gggctttgcc agaaagcagc
      841 gcggagcttt gagctccgcg cgggccatgc ccggccccat gtctggagcg cggcgataaa
      901 tcaccgcgct gcttgagagc gaagccctgt cgggctggat tgggctactc tcccgggcgc
      961 ctcagaacgg tgcgggatgg aagggggagc cccgaagagc tttgcctgga agcagcgcgg
     1021 agttttgaga tccgcgcgcg ggagtgggcc gcgccctccc tatccgtgtg cgttccctgc
     1081 atctgtggtg tcggtgcccg cgcgtggcat ccgggagtgc gagagagcta ccgtcctccc
     1141 gccatcagtg cgccctccat cggatccgcc tgcgacgccg gggccgcggg gaaggtgacg
     1201 gttacggttg tgcccttgcc ttcctgactc tcgatcccga tccggccgcc ttgagcctcg
     1261 acggcatgcc gacagaaggt gaggcccaat ccggtgccga cggcggcgcc tgacttgcgc
     1321 gccgcgacct gcgtgaactt atcgaagatc cgagaaagcc actccgcggg gatccccggg
     1381 ccctgatcgc tgatggagaa cgccacgccg cccgcgacgt tcgcgacgcg cagagtgatc
     1441 accgtctccg gcgggctgtg ccgaagggca ttccccagaa gattgaccag gacgcgctct
     1501 gccagggcgc ggtccgcgcg cactggcttg gcatcgggat ggtagtcgcg cgcgagggag
     1561 acacgggcgt tccgcgccca gagcgacacc tggttcagcg cgccatccgc cagctggcag
     1621 gggttcagat ccgccaggtg gatgggcatc cggtcgcctt ccatgcggga gatatcgagg
     1681 agcgcgtcga tcaggtgctt catgcgctcc gcggagatga gggcgatctc gccggcctcc
     1741 ctggggtcca cctgagccag ctcgtcgagg atcagcagcg tgtcgttgtg cagcgcggcc
     1801 agcgactcca gtccatttga ggtggcgcgc cagcggttga cgtagctggg gccaccaaag
     1861 agcgaagctg cagcagccaa ggcggtggtc ttgccggtcg aagaactacc cttgaggttg
     1921 ataccgcctg actcgatgcc agagaattcc agcagcatcg acgccaggcc cagcagcacc
     1981 gcggccagca tcttgccgct gaccagcgcg ccgcgcgccg cgggcgtggc cagcaacggc
     2041 tccagcgact ggcgctcgcg ttcgccggcg gtggtgtcca tcgccag
//
LOCUS       DAOSCV010000834         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35375_length_2081_cov_3.153011, whole genome shotgun sequence.
ACCESSION   DAOSCV010000834 DAOSCV010000000
VERSION     DAOSCV010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35375_length_2081_cov_3.153011"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PLY56_16800"
     CDS             <1..220
                     /locus_tag="PLY56_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOJ23187.1"
                     /translation="LSMIKGIMRQKKVPMLSDLIASARNAGVKLVACSMSMDLMGIKR
                     EELIDGVEVGGVAMYLNAAEQGTVNPFI"
     gene            complement(389..685)
                     /locus_tag="PLY56_16805"
     CDS             complement(389..685)
                     /locus_tag="PLY56_16805"
                     /inference="COORDINATES: protein motif:HMM:NF014479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HOJ23188.1"
                     /translation="MKFLVLWEIDIAHLGPEAIQAVMRMPEYAKRVSDQGKLTARYHV
                     VGKHGGAWIYDVQSNEELERLLATSPVYNRARFEVIPLAEMGAPTEVLQADGTA"
     gene            1057..>2081
                     /locus_tag="PLY56_16810"
     CDS             1057..>2081
                     /locus_tag="PLY56_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOJ23189.1"
                     /translation="MSDIARRFPTNPLLSPQDVRPSCEGLAVHCLLNPGVFRFRGKTW
                     LLLRVAEAPPATDEHVSTVIRTPEGDLEVLKYRRDDPDLDLSDPRVFRYRGEVLLTTL
                     SHLRLASSSDGVHFEPATSPTLEGFGLYETWGIEDCRVTEIEGRFYLTYTAVSPRGIA
                     VGMTSTEDWQEFTRHGNIFPPCNKDCALFPERVSGAYWALHRPASTGIGSHDIWIARS
                     PDLIHWGRHECLLRVRPGRWDSARIGAGASPIRTTAGWLEIYHGADENYRYCLGAVLL
                     DANDPTRVLARSEEPIMEPIAPYEQKGFFGNVVFTNGHVVDGDTVTLYYGASDCVICG
                     ATLSIRE"
BASE COUNT          370 a          659 c          657 g          395 t
ORIGIN      
        1 tctctcgatg atcaagggga tcatgcgtca aaagaaggtg ccgatgctct ctgacctgat
       61 cgcctccgcc cggaacgccg gcgtgaagct ggtggcgtgt agcatgtcga tggatctcat
      121 ggggatcaaa agggaggagc tgatcgacgg cgtggaggtg ggcggcgtgg caatgtacct
      181 caacgccgcc gagcagggca ccgtcaaccc cttcatctaa agcgcgcgtt cgccgaaaag
      241 gtcgccaggc tcccgagtgg gcgtccgtca cagggcgcga tgcgcaggca catgcggcac
      301 cccggcggcg gtggaaaagc aggatgctgt tgcaccgtca ccggggtgcc ggatagcagc
      361 ggcccgccag cgccataccg cacgttcgct aggcggtacc gtctgcttgt aacacctcgg
      421 taggcgcccc catctctgcc agggggatca cctcgaagcg ggcacggtta tacaccggcg
      481 acgtggccag taaccgctcc aactcctcat tcgactggac atcgtagatc cacgcgccgc
      541 cgtgcttgcc gaccacatgg tagcgcgctg tcaacttccc ctggtcactc accctcttcg
      601 cgtactccgg catgcgcatc accgcctgga tcgcctccgg gcccaggtga gcgatgtcga
      661 tctcccacag caccaggaac ttcactctgc tctccttccg cctgatccct gcggggttcc
      721 gagtgtgggc tctcctaccg ccagtgtagc atggagatgc tcacgttggc aagatcgctg
      781 gtccagtaac cacagacgga gggggcagac acggatgggg agggtatagg cgccatggac
      841 ctgctcacgc gctggcacat gaatgtttta tcgggagaat cttcagtgtt cgccgtgttt
      901 cccgtgaggc ggcagcttac ggcgctgcgg ccacgcggcg gagcggccct ctctcggaat
      961 cgcgcctgga accggttccc ttttacgctt acaattgtta taatggcgtg gggcgacatg
     1021 atggaggccc agactcttac ttagaggagt gacctattgt cagacattgc caggcgtttt
     1081 ccaacgaatc cattgctttc tccgcaagat gttcggccca gctgcgaggg attggccgtg
     1141 cattgcctgc tcaatccagg tgtgtttcgc ttccgcggca agacatggct gctcctgcgc
     1201 gtggcagagg cgccgccggc caccgatgag catgtcagca cggtcatccg caccccagag
     1261 ggcgatcttg aggtgctgaa gtatcgcaga gacgacccgg atctggacct gagtgatcct
     1321 cgcgtcttcc gctaccgggg agaggtgctg ctgaccacgc tctcgcacct gcgcctcgcg
     1381 agcagcagcg acggcgtcca cttcgagcca gcgaccagcc ccacgctgga agggttcggt
     1441 ctctacgaga cctgggggat tgaagactgc cgcgtcaccg agattgaggg ccggttctac
     1501 ctcacctata cggcggtatc gccgcgagga attgccgtcg gcatgacgag cacggaggac
     1561 tggcaggagt tcacccgaca cgggaatatc ttccctccct gcaataagga ctgcgcgctc
     1621 ttcccggaac gcgtctccgg ggcttactgg gcgctgcacc ggccggccag caccggcatc
     1681 ggcagtcacg atatctggat cgcgcgctcc ccggatctga tccactgggg caggcacgag
     1741 tgtctcctgc gcgtgcgccc gggcaggtgg gacagcgcgc gcattggcgc cggtgcctcg
     1801 ccgatccgga ccacggcggg gtggctggag atctatcacg gcgccgatga gaactaccgg
     1861 tattgcttgg gcgccgtgct cctggatgcc aacgacccga cgcgtgttct cgctcgctcg
     1921 gaagagccga ttatggagcc gatcgctccc tacgagcaga aaggcttctt cggcaacgtc
     1981 gtcttcacga acgggcatgt ggtcgacggc gacaccgtga ccctctacta cggcgcgtcc
     2041 gactgcgtga tttgtggggc cacgctctcg atccgcgaga t
//
LOCUS       DAOSCV010000835         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35492_length_2076_cov_3.333993, whole genome shotgun sequence.
ACCESSION   DAOSCV010000835 DAOSCV010000000
VERSION     DAOSCV010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35492_length_2076_cov_3.333993"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /gene="metG"
                     /locus_tag="PLY56_16815"
     CDS             complement(<1..1585)
                     /gene="metG"
                     /locus_tag="PLY56_16815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ23190.1"
                     /translation="MADKVYYITTPIYYVNDAPHIGHFSTTVAADVLARFQRLRGRQV
                     LFATGTDEHAPKVAEAARQRGLEPQAFVDEIVQAYLRTWEETHVSYDDFIRTSEPRHR
                     EVVSRVFQRLRDQGDIYKGVYEGWYCVPDETFWQESELIEGRCPNPECRRPVQKVQEE
                     NWFFRLSSYQDRLLEYYEAHPDVLQPEFRRNEVLSFIRGGLRDASITRSAYGWGIPVP
                     DDPSKVIYVWFDALLNYITVAGYLSDEEKFRDVWPADLHLMAKDIFVRFHATMWPAIL
                     MALDLPLPRRIFAHGFWTVDGEKISKSKGNAINPRKLAADVAERSGSRYDVAIDALRF
                     FIFREVPFGLDGDFSTSSFVQRFNADLANDLGNLVNRSITMLQRYFEGVVPAAEVDPA
                     IVAAAEPMLQGLPEAIEGLRFNVALDLIWGFIGALNKYIDERAPWKLAKEAQQDALAQ
                     VMYSLIDGIRLVALAVSPFMPEAAKEIWRQLGMAAPMEAQRWETAMRPGLLPAATATA
                     SPEPIFRRIEEKSAPAKEKK"
     gene            complement(1690..1923)
                     /locus_tag="PLY56_16820"
     CDS             complement(1690..1923)
                     /locus_tag="PLY56_16820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ23191.1"
                     /translation="MPLYEMKCADCRHTFEILTPTMAVPEGQQCPECGSTAVRRLFSP
                     FYSKTAQKAHTPSPEACRSCPGREAGSGCPYAE"
BASE COUNT          374 a          681 c          666 g          355 t
ORIGIN      
        1 tcttcttctc cttcgctggg gcgctcttct cctcgatccg ccggaagatc ggctcaggcg
       61 atgcggtcgc agtcgccgcg ggcaggaggc cggggcgcat cgccgtctcc caccgctggg
      121 cttccatcgg cgcggccatg cccagctggc gccagatctc cttcgcggct tcgggcatga
      181 agggcgaaac cgccaacgcc accaggcgga tgccgtcgat cagggagtac atcacttgcg
      241 ccagcgcatc ctgctgcgct tccttcgcca gcttccacgg cgcgcgctcg tcgatatact
      301 tattcagcgc gccgatgaag ccccagataa ggtcgagcgc cacgttgaag cgcagcccct
      361 caatcgcctc gggaagaccc tgcagcatcg gctccgccgc cgccacgata gccggatcca
      421 cctcggccgc gggcaccacg ccctcgaagt agcgctggag catggtgatg ctccggttta
      481 ccaggttgcc cagatcgttt gccagatccg cgttgaagcg ctggacgaag ctcgatgtgg
      541 agaagtcgcc atccaggccg aagggcacct cacggaagat aaagaagcgg agcgcgtcga
      601 tggcgacgtc gtagcgcgag ccggagcgct cggccacgtc ggcggccaac ttccgcgggt
      661 tgatcgcgtt ccctttcgat ttggagatct tctcgccgtc gacggtccag aagccgtgcg
      721 caaagatccg ccgaggcagc ggcaggtcga gcgccatcag gatagcaggc cacatcgtgg
      781 cgtggaagcg gacgaagata tctttggcca tcaggtggag gtcggccggc cacacgtcgc
      841 ggaacttctc ttcgtcggag agatagcctg ccaccgtgat gtaattgagg agggcatcaa
      901 accagacgta gatcaccttg gatgggtcgt ccggcacggg gatgccccag ccgtaggcgg
      961 aacgcgtgat gctggcatcg cgcaggcccc cgcgaatgaa ggagaggacc tcattgcgtc
     1021 ggaactccgg ctgaagcaca tccgggtggg cctcatagta ctccagaagg cgatcctgat
     1081 agctcgaaag ccggaagaac cagttttcct cctgcacttt ctgaacaggg cggcggcact
     1141 cggggttggg gcagcggccc tcgatcagct ccgactcctg ccagaacgtc tcatccggca
     1201 cgcagtacca gccttcgtaa acacctttgt agatgtcgcc ctgatcacgc aggcgctgaa
     1261 agacgcgcga gaccacctcg cgatgccgag gctcactggt gcggatgaaa tcatcgtagg
     1321 agacgtgcgt ttcctcccac gtgcgcagat aggcctggac aatctcgtcc acaaacgcct
     1381 gcggctcgag gccacgctgg cgagcggcct ccgctacctt gggggcgtgc tcgtcggtgc
     1441 ccgtcgcaaa gagcacctgg cgcccgcgca ggcgctggaa acgcgcgagc acgtcggccg
     1501 ccacagtcgt cgagaagtgg ccgatgtgtg gcgcatcgtt cacgtagtag atgggcgtgg
     1561 taatgtagta aactttatct gccatgtatc tctcccgcca cggggttccc gggggaatcc
     1621 gccccagagt gcggcgcgaa gcggcgatgc cgccccggcg ctcgctcctc ctggcctctc
     1681 ggggattgcc tactcagcgt acgggcaacc cgatcccgcc tcgcgaccgg ggcaggagcg
     1741 gcacgcctcc ggcgatgggg tgtgcgcctt ctgcgccgtc ttcgagtaga aaggcgagaa
     1801 gagacggcgc accgccgtgc tgccacactc cgggcactgc tgcccctcgg gcactgccat
     1861 ggtcggcgtc aggatttcga acgtgtgccg gcagtcggcg cacttcatct catagagcgg
     1921 cattcgatcc tccccaagaa gagggcagca ggccggcggc gccgggaacc tgctgcccgc
     1981 gaaacggtgt acacgaaagg agcgaagctc cgcggggcga gacaccgcgg ctcattcccc
     2041 gacggttgtt cccgcggatg cttcgcccct acttcc
//
LOCUS       DAOSCV010000836         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35574_length_2073_cov_3.729435, whole genome shotgun sequence.
ACCESSION   DAOSCV010000836 DAOSCV010000000
VERSION     DAOSCV010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35574_length_2073_cov_3.729435"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..1435
                     /locus_tag="PLY56_16825"
     CDS             377..1435
                     /locus_tag="PLY56_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOJ23192.1"
                     /translation="MRDESLAFLRRLLEAPSPSGYERPAQDVVRDWAGSFADEVRTDV
                     HGNVIAAVNPSGRPRIMLAGHCDQIGLMVQHIDDDGYLYVNQIGGHDITVLIGQNVVV
                     WTKNGPVSGVMARKPIHLMKDDEEKKPPKIHDLWVDIGAANKQEALEAVEIGDPITWS
                     LGMRTLRNDLAASPAFDDKAGVYVVMEALRLLTEERPTAAVFAVSTVQEEIGLRGAVT
                     STFAVEPQVGIAVDVTFATDHPGADKKVSGDVSLNKGPAIHRGPNMNPVVVSRLIAAA
                     KEKAIPYQLVGSARATGTDANVMQISRGGVATGLVSVPNRYMHSPVETVSLRDMQHAA
                     ELLAAFILKVEPDAEFIP"
     gene            1564..>2073
                     /locus_tag="PLY56_16830"
     CDS             1564..>2073
                     /locus_tag="PLY56_16830"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ23193.1"
                     /translation="MLEKETLIRLLRTMYRIRFFEERLKKHYDYRAYFERPASGARDT
                     GDLIGADGYDFASHGLIGGAVHLSIGQEAVSAGIGAHLGPRDAAFSSHRSHGHALAKG
                     ADVKAAFAELMGRKTGLSRGYGGSMHLFDPANGFLGGNGIIGGQIPLALGPAFAAKYR
                     GEPHVSVVFF"
BASE COUNT          348 a          683 c          694 g          348 t
ORIGIN      
        1 cgacacgccg ccgcaggcac gctttccttt gtgcaagagg cgtaccactc tgcccctgaa
       61 cgcctccccg gcgaaggagg gcgcccgcga agcggcgaac agaagccccg gggtgctgcc
      121 tgctcctcag gggggtggag ctcactcatc ctggattgcc acgctggttc gcacgctccg
      181 atgctccctt ggccccggat cacggctgcc cgacgcatgg cgcctggccg ggggctcatc
      241 cctggaaagg ggcgccccat gatgaagccc gctttcacga ggcggaggca tccctcgatt
      301 ggctgcccgg tgcggattgc cgggccagag ggcgcctcac tctccgcatc gcacccgtca
      361 ggggggagga tagagaatgc gtgatgagtc gttagctttc ctgcgccgtc tgctggaggc
      421 ccccagccct tccggatacg agcgcccggc ccaggacgtg gtccgcgatt gggctggatc
      481 gtttgctgat gaagtgcgca cggacgtcca tggcaacgtc attgccgccg tgaacccgtc
      541 ggggcgccca cgcatcatgt tggccggcca ctgcgaccag atcgggttga tggtccagca
      601 tatcgacgac gacggctacc tctatgtcaa ccagatcggt ggccacgata tcaccgtcct
      661 catcggccag aacgtggtgg tgtggacgaa gaacggccct gtgtccggcg tgatggcgcg
      721 caagccgatc cacctgatga aggatgacga ggagaagaag ccccccaaga tccacgacct
      781 ctgggtggat atcggcgcgg ccaacaagca ggaggcactc gaggcggtgg agatcggcga
      841 cccgatcacc tggagcctcg gcatgaggac gttgcgcaac gacctggccg cttcccccgc
      901 cttcgacgac aaggccggcg tctacgtcgt catggaggcg ctccggcttc tcaccgagga
      961 gcggcctacc gctgcggtct tcgctgtctc gacggtccag gaggagatcg gcctgcgtgg
     1021 cgcggtcacc agcaccttcg ccgtggagcc ccaggtgggt atcgccgtcg atgtgacctt
     1081 cgccactgac catcccggcg cagataagaa ggtgagcggc gatgtctctc tcaacaaggg
     1141 gccggcgatc caccgcggcc cgaatatgaa ccccgtggtc gtctcccgcc tgatcgccgc
     1201 ggccaaggag aaggcgattc cctaccagct ggtgggatcc gcgcgggcga ccggcaccga
     1261 tgccaacgtg atgcagatca gccgtggcgg cgtcgcgacc gggctggtga gcgtgcccaa
     1321 ccgctacatg cacagcccgg tggagacggt ctcgctacgc gatatgcagc acgccgctga
     1381 gctcctggcg gctttcatcc tcaaagtgga gccggacgcg gagttcattc cctgacagcg
     1441 ctctaaggtt cgcgcgggca aggagcgcat ggtggcagcg cgctccttgc cacgctctgt
     1501 cacggttcag gcgcgaggcc tctcgccgat agccctcgcc accttcccgg agggagagag
     1561 cgaatgcttg agaaggagac actcatccgg ttgctgcgga ccatgtaccg catccgcttc
     1621 ttcgaggagc gcctgaagaa gcattacgac taccgggcgt atttcgagcg gcccgcttcg
     1681 ggagcgcgcg atacgggcga cctcatcggc gcggatggct atgacttcgc aagccacggg
     1741 ctgatcggtg gcgcggttca cctctccatc gggcaggagg ctgtctcggc cggcatcggc
     1801 gcgcatctgg ggccccgaga cgccgccttc agcagccacc gcagtcacgg gcacgcgctc
     1861 gccaagggcg ccgatgtgaa ggctgcgttc gcggagttga tgggtaggaa gacggggctc
     1921 tcgcgcggct atggcggctc gatgcacctt ttcgacccgg cgaatggctt cctggggggc
     1981 aacggcatca tcggcgggca gatcccgctg gcgctgggcc cagcgttcgc ggcgaaatac
     2041 cgcggcgagc cccacgtctc ggtcgtcttc ttc
//
LOCUS       DAOSCV010000837         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35608_length_2072_cov_2.600397, whole genome shotgun sequence.
ACCESSION   DAOSCV010000837 DAOSCV010000000
VERSION     DAOSCV010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35608_length_2072_cov_2.600397"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PLY56_16835"
     CDS             complement(<1..1652)
                     /locus_tag="PLY56_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ23194.1"
                     /translation="MSLLSVGELSKSYGTQEVLRSVSFIVGPGEKVALVGRNGCGKST
                     LLRLIAGKEEPDAGRVSLLPRTHIGYLSQEPDLSAGTTLLDAVTWAVPRLVELERRLQ
                     ELEARLEGHPSDAEELYAEYAEAQQEFAQAGGPAYAGRVKATLAGLGFRESDYELPVT
                     ALSGGQRTRAALAMLLLQEPDLLLLDEPTNHLDLPAIEWLEEYLTRTRSALILVSHDR
                     YFLDRVVTRVLELEEAEIHSYPGNYTAYSRIREERLARQQAVYERQLQQVEKLEEYIR
                     RYGAGNRATMAKSREKALARIQLEPPRTRKAEMRLDLSPGLRSGTEVVCIERLSKSYD
                     GKQLFSGLELVVRRGERVGIVGPNGSGKTTLLRILMGQVDADAGHFAFGHNVEPGYFA
                     QDLSGANPENTLLEEILDAGAATVEDARSLLARFLFFGDDVFKRVGDLSGGETNRMAL
                     ARLLLSHANLLLLDEPTNHLDIAARDRLEEALLAFPGTVICASHDRYLLDRIATRIVE
                     VAGGEARVYDDTYAEYRRRKERERLLAEQAAARAAKAAPPPPP"
BASE COUNT          352 a          731 c          650 g          339 t
ORIGIN      
        1 ggtggcgggg gaggcgcggc cttcgcggcc cgcgcggccg cctgttccgc caacaggcgc
       61 tcgcgctcct tgcggcggcg gtactcggcg taggtatcgt cgtacactcg ggcctcgccg
      121 cctgcgacct cgacgatccg cgtcgcgatc cggtcgagca ggtagcggtc atgcgaggca
      181 cagatcacgg tgcctgggaa agcaagaagc gcctcttcga gccgatcgcg cgcggcgata
      241 tcgaggtggt tggtcggctc atccaggagc aggaggttgg cgtgcgagag caacagccgc
      301 gcgagcgcca tgcggttcgt ctctcccccg ctcagatcgc ccactcgctt gaagacatcg
      361 tcgccaaaga agaggaagcg tgccagcagg ctgcgcgcat cctcaacagt ggcggctcca
      421 gcgtcgagaa tctcctctag gagcgtgttc tcggggttcg cgccgctcag gtcctgggca
      481 aagtagccgg gctccacgtt gtgtccaaag gcaaaatgcc ccgcatcggc gtcgacctgg
      541 cccatcagga tccgcagtag cgtggtcttc ccggagccgt tcggaccgac gatgcccacg
      601 cgttcgcccc gccggaccac cagttcgaga ccggaaaaga gctgcttacc gtcgtaactc
      661 ttcgagagcc gctcgatgca gaccacctcg gtgccgctcc gcaggcccgg gctgagatcc
      721 agacgcatct ccgctttccg agttcgcgga ggttccagct ggatgcgcgc cagcgccttc
      781 tcccggctct tggccattgt cgcccggttt cccgcgccat agcggcggat atactcttcc
      841 agcttctcca cctgctggag ctggcgctcg tagacggctt gctggcgcgc taggcgctct
      901 tcccggatgc gagaataggc cgtgtagtta ccggggtagc tgtggatctc ggcctcctcc
      961 agctcgagaa cacgcgtgac cacgcgatcg aggaagtagc ggtcgtgcga aacaaggatc
     1021 agcgccgacc gggtccgcgt caggtactcc tccagccact cgatggcggg gagatccagg
     1081 tggttggtgg gctcatccag cagcaggagg tcgggctcct ggaggagcag catcgccagt
     1141 gcggcacgcg tgcgctgccc gccactcagc gcggtgacgg ggagctcgta atcgctctca
     1201 cggaaaccga gaccggccag ggtggccttc acccggcccg cgtaggccgg gccgccggcc
     1261 tgagcaaact cctgctgcgc ctccgcgtac tccgcgtaca gctcctcagc atcggacggg
     1321 tggccctcca ggcgcgcttc cagctcctgc aggcgtcgct ccagctccac cagccgcggc
     1381 acggcccagg taaccgcatc cagcaaggtc gtgccggccg agaggtccgg ctcctgtgac
     1441 agatagccga tgtgcgtgcg cggcagaagc gacactcgcc cggcgtccgg ctcctctttc
     1501 ccggcgatca ggcgcaacag ggtactcttg ccacagccgt ttcggcccac cagggccact
     1561 ttctcgcccg gcccaacgat gaacgaaacg gatcgaagca cttcttgcgt gccgtagctc
     1621 ttggaaagct ctccaactga aagcagactc atagcagcag atcgaaaggc acccgcgccg
     1681 ttccacccgc gcggcgcgcg tcgtaacccc tcccccagtc ctttctcagc atcgtcccac
     1741 ggcagcgcgc cagggcaccc ccgcgatccg ggaggcctcc cgtaacctgg atggacgcct
     1801 ctcccagcgg acggccctcc gcgacggtct ctattgtatc atgacacacc ccaaaacgcc
     1861 agtgacagcc cctggatgga ggaccagggc tcgctcgcgc atcgcgggcc cggataccac
     1921 gaaagagggg gaaaggcacg gatgaggagg atgcgcgccc ggcgcacgga gctcgaagct
     1981 ccgcgctgct tccctgcaaa gcccctatcg aagctccccc ttccagctcc tgctagctcc
     2041 cgttgcccag gactgtagcc taacccagcc cg
//
LOCUS       DAOSCV010000838         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35636_length_2071_cov_2.990575, whole genome shotgun sequence.
ACCESSION   DAOSCV010000838 DAOSCV010000000
VERSION     DAOSCV010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35636_length_2071_cov_2.990575"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1436
                     /locus_tag="PLY56_16840"
     CDS             99..1436
                     /locus_tag="PLY56_16840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008692844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOJ23195.1"
                     /translation="MASETAVAAGVLARNAIDARILAAMTGRQGSGAMNTKIEQAREV
                     ALGILKPSQRDLEYGLGLHREAVVIDGYGFSPRAAIDGEALRALVEEGASEAEITDSI
                     EEMTMTRAATDPVQREEYLAAWNAAGVTCIVQNAGEEGQSPQRLLKRLARFTYTTDCL
                     RGTVSRAAFPDDILRTKEAGAHCLYFTGNGVPLAQEWVSPEEELRFVRLFFQLGIRMM
                     HLTYNRRNMIGDGCAERANGGLSDFGHAVIAEMNRVGVIVDVAHSGWQTSLEAAKASR
                     VPMVASHSGCCAVNTHIRCKPDEVIRAIVETGGYIGICWVPAFLGGSGDISALLDHVE
                     YVARTFGADYVALGSDTAYTAAAYEDEWKKVPPRRRSRPRWEGFWPKDDPLFDSTWHQ
                     PHQVQSLAWTNWPLITVGLVQRGFSDEEIRKILGGNVLRVARAVLEAAGVATS"
     gene            complement(1512..1799)
                     /gene="csrA"
                     /locus_tag="PLY56_16845"
     CDS             complement(1512..1799)
                     /gene="csrA"
                     /locus_tag="PLY56_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017935775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOJ23196.1"
                     /translation="MLILSRKAGQGLVIGEDVEITVLEIRGDVVRLGIRAPREVSVHR
                     TEVYLQIAEANRNAAAVEKEALSRAAGIRPPDHPGAEQPPGLSVVVRRPAA"
     gene            complement(1813..>2071)
                     /locus_tag="PLY56_16850"
     CDS             complement(1813..>2071)
                     /locus_tag="PLY56_16850"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOJ23197.1"
                     /translation="RWFPDYEVILRNEDAAALGIEDPDDAGILAIVTLPARLEEMTAN
                     LLGPIVVNVRTRRAKQVVLDDPRYHTRHRLLPQPLTATCAA"
BASE COUNT          348 a          647 c          721 g          355 t
ORIGIN      
        1 cagctcccgc gttgcgttat caggggtagc gcgcggcgcg tccgctgcag gaagggcatg
       61 gccctctggg gaacgggttg gcaagggaga gcgccgctat ggcgtctgag actgccgtgg
      121 cggccggagt gcttgcgcgc aacgcgattg atgcgcgcat cctcgctgcc atgacgggca
      181 ggcaagggag tggagcgatg aacacaaaga tagagcaggc ccgggaagtg gccctgggca
      241 tcctcaagcc aagccagcga gacctggagt atggattggg cctgcatcgg gaggccgtgg
      301 tgatcgatgg ctacggcttc tcgccgcgcg ccgccattga tggcgaggcg ctcagggcgc
      361 tggtcgagga gggcgcctcc gaggcggaga tcacggatag catcgaagag atgaccatga
      421 ctcgcgcggc caccgatcct gtacagcgcg aggagtacct ggcggcgtgg aacgcggcgg
      481 gcgtcacctg catcgttcag aacgcgggcg aggaggggca gtcgccccag cggctgctca
      541 agcggttggc acgcttcacc tataccaccg attgcctgcg tggcaccgtg tcgcgtgcgg
      601 ctttccccga cgatattctg cgcacgaaag aagcgggggc gcactgcctc tatttcaccg
      661 gcaacggtgt gccactggcg caggagtggg tctcgccgga ggaggagctg cgcttcgtgc
      721 gcctcttctt ccagcttggc atccggatga tgcacctcac ctacaaccgg cgcaatatga
      781 tcggcgatgg ctgcgccgag cgtgccaacg gcgggctgag cgacttcggt catgccgtca
      841 tcgccgagat gaaccgcgtg ggcgtgatcg tcgacgtggc ccactcgggg tggcagacca
      901 gtctggaagc ggccaaggct tcccgcgtgc cgatggtggc cagccattcc gggtgttgcg
      961 cggtgaatac gcatatccgc tgcaagccgg atgaggtgat ccgcgccatc gtcgagaccg
     1021 ggggctatat cggcatctgc tgggtgcctg ccttcctcgg aggcagtggc gacatttccg
     1081 cgctcctcga tcacgtggag tatgtggccc gcaccttcgg cgcggattac gtcgcgcttg
     1141 gctcggatac cgcgtacacc gccgcggcgt atgaggatga gtggaaaaag gtgcccccgc
     1201 gccgccgttc ccggccgcgc tgggagggct tctggcccaa agatgatcct ctctttgact
     1261 ccacatggca ccagccgcat caggtgcaga gcctggcgtg gacgaactgg ccgctgatca
     1321 ccgtgggact ggtgcagcgt ggcttcagcg atgaggagat ccggaagatc ctgggcggca
     1381 acgtcctccg cgtggcacgc gccgtgttgg aggccgcagg agtggcaacg tcctaaacga
     1441 aaggcatggt ggatcgggcg catcctttcg cccgttccac cgcgcccgcc cgtcaagggc
     1501 aggcgtcgag ctcaggccgc cgggcggcgg accaccacag agaggccagg cggctgctct
     1561 gcccccggat gatctggcgg acggataccg gccgcacggc tgagcgcctc cttctctacg
     1621 gcggcggcat tccggtttgc ctcggcgatc tggaggtaaa cctcggtgcg gtgaacggag
     1681 acctcgcggg gcgcgcggat gcccaggcgc accacatccc cgcgtatctc gagaacggtg
     1741 atctcaacat cctcgccgat caccaggccc tgcccggcct tccgcgagag tatcaacatc
     1801 gcctcgctcc gttcaagcgg cacacgttgc ggtcaagggt tgtgggagaa gccggtgccg
     1861 ggtgtggtat ctcgggtcgt ccagcacgac ctgctttgcc cgtcgggtgc gcacgttgac
     1921 cacgatgggg cctagcaggt tggcggtcat ctcctccaaa cgcgcgggca aggtgacgat
     1981 ggcgagtatg ccggcatcat ccgggtcctc aatgcccagc gccgcggcgt cctcattgcg
     2041 cagaatcact tcgtaatcgg ggaaccaccg c
//
LOCUS       DAOSCV010000839         2067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35728_length_2067_cov_3.950298, whole genome shotgun sequence.
ACCESSION   DAOSCV010000839 DAOSCV010000000
VERSION     DAOSCV010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35728_length_2067_cov_3.950298"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            286..1338
                     /locus_tag="PLY56_16855"
     CDS             286..1338
                     /locus_tag="PLY56_16855"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOJ23198.1"
                     /translation="MHAPRAVSSRGFSLLQLIVALGIFAVLMTVAFRAYSNARASAVA
                     SVCSGRLKAIAMDLERYRVDHHAYPAVLDELYPTYTKSEEALRCPEENRPDVRTYSDF
                     YVPRDPKEQHRDRLVLSCPFHQETGKGIEVFLGDVAVHERGKTYVATLTGGAGLASVL
                     PYDAERPWDAEPEDPEFWANAIAALPNMEVGPGDWVRVPSGGVTLNFKDGSRAEITGP
                     AEVMVVDCFRSTAERGEPSPFYTILRLARGQVYNIVIPGSKYEVVTPTGTAGARGTEF
                     LVTYVSEGVGAPLHPGKGKGKGLAKGKFKPKGKASTEVVRGKVYLTGRYTTVELAEGD
                     SAEVDERGNAKKKKKK"
     gene            1393..>2067
                     /locus_tag="PLY56_16860"
     CDS             1393..>2067
                     /locus_tag="PLY56_16860"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOJ23199.1"
                     /translation="MWIGCLAAVIASLLYGIFRLERWEFASLDARLLWRQENRLRDSD
                     IQTLVVAIDDKTLGMMPNRRWPFPRSEYARLVKVLKDAGARCLGFDLRFEDSDPPEDQ
                     QLAAALRAAGNVYLGVRFDPQSSIFPGPMEIGDALDASLIATDPEGSARIKLKMESAA
                     LLPEVLARSARGLGSITVVPELADGMLRRFPVVVAYVPPGDTRVRLYASLPLLMAADH
                     LGVPLSE"
BASE COUNT          357 a          650 c          687 g          373 t
ORIGIN      
        1 ggaggccgcc tggcaaagga ctctcaagaa ggacgaagaa tatgccataa tggtggatga
       61 gtgagtgggg gccgctccca gaaggggcca ggcggcgcct tctcgatcat accggtcgcc
      121 atcaaggcga gaccacatag cccggttccc ctgcctgccc acgatgcggg gtgcggttga
      181 atcgacagcg cagagccgtc agtgcattgg tccgcctcca tgagccatcc ggatgtgggc
      241 cagttttgcc gcgggcaacg ccgtttgttc atgaaaggag ggcgtatgca cgcccctcgt
      301 gccgtatcct cgcgcgggtt ctcgctgctg caactgattg tggcgctggg catattcgcg
      361 gtcctcatga cggttgcgtt cagggcctac tccaatgcac gcgccagcgc cgtggcgagc
      421 gtttgctctg gccgcctcaa ggcgattgcc atggatctgg agcgctatcg ggtggaccac
      481 cacgcctatc ccgcggtcct cgacgagctc tacccaacat acaccaaatc cgaggaagcc
      541 ttacggtgcc ccgaggagaa ccgccccgac gtgcgcacct acagcgattt ctacgttcct
      601 cgcgacccca aggagcaaca ccgcgaccgc ctggtcctct cctgcccgtt ccatcaggag
      661 accggaaagg ggatcgaggt tttccttggc gatgtggccg tgcatgagcg gggcaagacg
      721 tatgtcgcca cgctcaccgg tggcgcgggc ctcgcgagtg tgctccccta cgacgcggag
      781 cgcccctggg acgccgagcc ggaagatccc gagttctggg ccaatgcgat tgccgcgctg
      841 cccaacatgg aagtcggccc aggcgactgg gtgcgcgtgc cgtccggtgg ggttacactc
      901 aacttcaagg atggttcccg agccgagatc accgggccgg ccgaggtcat ggtcgtggat
      961 tgctttcgct ccaccgcgga gcggggagag ccttctccct tctatactat cctgcgactg
     1021 gccagggggc aggtctacaa catcgtgatc ccaggctcca agtatgaggt ggtgacgccg
     1081 acgggcacgg ctggcgcccg tggcaccgag ttcctggtca cctatgtctc cgaaggggtg
     1141 ggggcgccgc tgcatcccgg caagggcaaa ggaaagggac tggccaaggg gaagttcaag
     1201 cccaaaggga aagcctcgac cgaggtggtc cgcggcaagg tgtatctgac cggccggtat
     1261 acgaccgtgg agctggcgga gggcgacagc gcggaagtgg atgagcgcgg caacgccaag
     1321 aagaagaaga aaaagtagcc gtcggtgtct tctcgcgtag ttcccggagc gtctcgcaaa
     1381 gcagtgcgcg gcgtgtggat cggctgcctc gccgcggtca tcgcgtctct cctctacggc
     1441 atcttccgtc tggagcggtg ggagttcgcg tccctggatg cgcgcctgct ctggcgccag
     1501 gagaaccgct tgcgtgatag cgatatccag acgttggtgg tcgctatcga tgacaagacc
     1561 ctgggcatga tgcccaaccg gcggtggcct ttcccgcgca gtgagtacgc ccggctggtc
     1621 aaagtgctca aggacgcggg cgcgaggtgc ctcggcttcg acttgcgctt cgaggattct
     1681 gatccgccgg aggaccaaca gcttgccgca gcactgcgtg ctgctggcaa cgtctatctc
     1741 ggagttcgtt tcgacccgca atccagcatc ttcccgggtc cgatggagat cggcgacgcg
     1801 ctcgacgcgt cgctgattgc cacggacccg gaggggagcg cgcgcatcaa gctgaagatg
     1861 gagagcgcgg cgctcctccc cgaggtgctc gcccgatcgg ctcggggcct tggctctatc
     1921 acggtcgtgc ctgaactggc agacggaatg ttgcgccgct tcccggtggt ggtcgcctac
     1981 gtgcctccag gcgacacgcg cgtccgcctc tacgccagcc tccccctgtt gatggccgcc
     2041 gaccacctgg gcgtgccgct cagtgag
//
LOCUS       DAOSCV010000840         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35789_length_2065_cov_3.765672, whole genome shotgun sequence.
ACCESSION   DAOSCV010000840 DAOSCV010000000
VERSION     DAOSCV010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35789_length_2065_cov_3.765672"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1313
                     /locus_tag="PLY56_16865"
     CDS             249..1313
                     /locus_tag="PLY56_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ23200.1"
                     /translation="MSYPEVRKAILLVAGLYLLYRFVGTVTAILFLFFAVFILAMALN
                     PIVNWLAARRVPRAAGAVLIALALLALLTVAALVIVPPMVAQGEQFVRDAPGYWAAAQ
                     SRVERILERFPVVRERLATDGQLGQEIVQHASNLIRSAGRYTVTAAGLVFALLLALIL
                     TVYALAQPCPLVKGLLGAVPAEHREKTRTALIRIASQVRAWVVASVLLGAVVAFVAWL
                     GLTLLGVPNALLLAMLAFFGEAVPNFGPIVAAIPAILIAFAVSPITALWVAVLYVAIQ
                     QVESYLLAPLIMGKQLHLHPITVAFFVLVMGALAGLIGAILAVPTAAIARILYEEFYY
                     KPRHPDEEAITRDANEVIAA"
     gene            complement(1323..1796)
                     /locus_tag="PLY56_16870"
     CDS             complement(1323..1796)
                     /locus_tag="PLY56_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23201.1"
                     /translation="MLAAVLLAGVLLGWLVIPRGGSPAEEERIRETLEMARQAVEAHN
                     AGRFMRYVSTRYDDGSYKYETLRSMLSLGLREYGQVRVVLYVRDIQVRGERATAEMEV
                     QAEGIDPSGNRVRFEGPMKVELVRETARRRLFFSESRWMAISTDATSALQDAFVQ"
     gene            complement(1865..>2065)
                     /locus_tag="PLY56_16875"
     CDS             complement(1865..>2065)
                     /locus_tag="PLY56_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HOJ23202.1"
                     /translation="LRLVGSNCYGLIDGRSDFALAIDLLASGKVDPAPLVTHRVPLAS
                     IQEAFETALDKSTGAIKVWVEP"
BASE COUNT          308 a          696 c          662 g          399 t
ORIGIN      
        1 gatttatcgc cgcgcgctgg gttgcgcact gcccccgtgg cttcggattc cttatgtcgg
       61 aatctcagca accgagggaa ctgcccgccc tgttgcctct cgcccaaatc cggttgccca
      121 cgggatcggc agccggaacg ggacgcgcgc gttggcatga catctgcctg tgtcgttcgc
      181 atctacccgc gcgggggtat aaggatcaca ccgtctgatc cgggtgattc ggccaggggg
      241 atgtgtcgat gagctatccg gaagtgcgca aggcgatctt gctggtggcc gggctttacc
      301 tgctctaccg gtttgttggc acggtgacgg cgatcctctt cctctttttt gccgtcttca
      361 ttctggcgat ggcgctcaac ccgattgtca actggctggc ggcacgccgg gtcccgcggg
      421 ctgccggcgc cgtcttgatc gcgctcgctc ttctggcgct gctgacggtt gcggcactgg
      481 tgatcgtgcc cccgatggta gcgcaggggg agcagtttgt tcgcgacgcc ccagggtatt
      541 gggcggccgc gcaatcccgg gtcgagcgca ttctcgagcg gttccccgta gtgcgtgaac
      601 ggctggcgac ggatggccag ttgggccagg agatcgtgca gcatgccagc aacctgatcc
      661 gatccgccgg gcgctatacg gtgaccgccg ccggcctggt tttcgcactg ctgctcgcct
      721 tgatcctgac ggtctatgcg ctcgcgcagc cgtgccctct cgtgaaagga ctcctgggcg
      781 cggtgcctgc agagcatcgc gagaagacgc gcacggcgct catccggatc gccagccagg
      841 tgcgcgcgtg ggtggtcgcc tcggtgctgt tgggcgcggt ggtcgccttc gtggcgtggc
      901 ttgggctcac cctcctgggg gttccaaacg ccctgctcct ggccatgctg gcgtttttcg
      961 gtgaagccgt gccgaacttc ggccccatcg tggccgccat ccccgcaatc ttgatcgcct
     1021 tcgctgtcag cccgatcacc gcgctctggg tagcggtgct gtatgtggca atccagcagg
     1081 tggagagtta cctcctggcg cccctcatca tgggaaagca gctccacctg catccgatca
     1141 cggtcgcgtt tttcgtgctg gtgatgggag cgctcgcggg cctcatcggg gcgatcctcg
     1201 cggtgcctac cgcggcgatc gcgcggattc tctacgagga gttctactac aagccgcgcc
     1261 acccggacga ggaggcgatc acccgcgacg ctaacgaggt cattgccgct tgaaaccggt
     1321 gttcactgca cgaaggcatc ttgtagggcg ctggtcgcat cggtggagat ggccatccag
     1381 cggctctcac tgaagaagag gcgccggcgc gccgtctcgc ggacgagctc caccttcatc
     1441 gggccctcga agcgcacccg attccccgat gggtcgatcc cctcggcctg cacctccatc
     1501 tccgccgtgg cgcgctcccc ccgcacctgg atgtcgcgca cgtagaggac gacgcgcacc
     1561 tgaccgtatt cgcgcaggcc aagggagagc atgctccgca gcgtctcata cttgtagctg
     1621 ccatcatcgt agcgcgtcga aacataccgc atgaagcgcc cggcgttgtg cgcttccacg
     1681 gcctgtcgcg ccatctctag cgtctcgcga atccgctcct cttccgcggg cgaaccgccg
     1741 cgcgggatca ccagccaccc gagcagcact cccgccagaa gaacggccgc cagcacaagg
     1801 cgtttcaggg cacaccacct cccgccgaat gcgagcccat tgatctcacc tccgcggcgc
     1861 tcgcctacgg ctcgacccac accttgatcg cgccggtcga cttatccagg gccgtctcaa
     1921 acgcctcctg gatcgacgcg agaggcacgc ggtgggtgac cagtggcgcc ggatccactt
     1981 tgccggacgc gaggaggtcg atggccaggg caaagtcgct gcgcccgtcg atgagcccgt
     2041 agcagttgga gccgacgagg cggag
//
LOCUS       DAOSCV010000841         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_35893_length_2061_cov_2.850449, whole genome shotgun sequence.
ACCESSION   DAOSCV010000841 DAOSCV010000000
VERSION     DAOSCV010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35893_length_2061_cov_2.850449"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..>2061
                     /locus_tag="PLY56_16880"
     CDS             346..>2061
                     /locus_tag="PLY56_16880"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOJ23203.1"
                     /translation="MKQLALALALLAWAVPDGVGAAEATLPVSKVTVFSSGVAYFEHN
                     GRVTDAAEVTMRFKTEGINDLLKSLVVMDLGGGSVSSVNYASREPLERALKGFGVDLT
                     GQPTLPELLRQLRGATVSVRTPELASGKILAVRSQKKQLLPGNVLVEEQILELVTEEG
                     LKAIPLDSIRSVSLADARLNAELQKALQLLAENRDVDTRPVTLRFAGKGERLVRVGYV
                     AEAPVWKSSYRLVLDEAKGGALLQGWAVVENTSDLDWEGVTLTLASGRPISFIQDLYT
                     PLYLSRPVVQSERYASLRPQEYAEGMETLKAPASSKAMQRREASGKPPAVMAPAPAST
                     AIAADAGAANGAMRSDLQPAATGETAGELFVYQMKEPVTIPRRQSAMLPIINQKVQAR
                     KVSIYNQRALANHPLSGVWLTNNTDLTLLAGPVTLLDGGTYAGDALLGNITPKERRLL
                     SYAVDLKTTVDSSVKSSKKIASARIVRGTMTITHKRYFTQTYSLKNKADEERVVIIEH
                     PFNPERTLIEPKEPTEKTPELYRFEVTVPAGATRAFDVREEQTEMEMMDLLPADLGTL
                     QMYASS"
BASE COUNT          384 a          669 c          690 g          318 t
ORIGIN      
        1 gtaccggtgg cgcgaccgga tcgcccgcgc cattgagacg acgggcacct cccctgccgc
       61 tccttgggac aggcccgcgc ccggcagagc gaaccccgag tgaaggatcc gggccgatgt
      121 agcgtcatag agagtagagg gcgtgctctc gcgagtcatc tccaggagcg ctggagtgcg
      181 gcccgaccgg atcgatgcct gggagtcttc gcgccggcgt ttgggaagca cgttgccctg
      241 cgcgacggag ccctcttgca tgagacggtg agatcgcgga acgaaacgag agaggaggac
      301 gggaggggca gggggcccat cgccccttgc atccccggga tctttatgaa acagctagct
      361 ctcgccctgg cgctgctcgc atgggcggtg ccggatgggg taggcgcggc ggaggcgact
      421 ttgcccgtct cgaaagtgac ggttttcagc tctggggtgg cctacttcga gcacaacggg
      481 cgcgtgaccg atgcggcgga ggtcacgatg cgcttcaaga cggaggggat caacgacctc
      541 ctgaagtcac tggtggtgat ggatctgggc ggaggcagcg tgagcagcgt caactacgcc
      601 tcgcgcgagc cgctggagcg cgcgctgaag ggcttcggcg tggacctcac cggacagccg
      661 accctcccgg agctcctgcg ccagcttcgc ggggcaacgg taagcgtacg caccccggag
      721 ttggcgtccg gcaagattct ggcagtgcgt tcccagaaga agcagcttct gcccgggaac
      781 gtgctggtcg aggagcagat cctggaactg gtgaccgagg agggcctgaa ggcaatcccg
      841 ctcgattcca tccgaagcgt ctccctggcg gatgcccgcc tgaacgcgga gctgcagaaa
      901 gccctccagc ttctcgcaga gaaccgcgac gtggatacgc gcccggtgac cctccgcttt
      961 gccggcaagg gagagcgcct ggtgcgcgtc ggctatgtgg ccgaggcccc cgtctggaag
     1021 agcagctatc gcctggtcct cgacgaggcg aagggcgggg cacttctgca aggttgggcc
     1081 gtcgtagaga acacgagcga tctggactgg gagggggtga cgctcaccct tgcttcaggc
     1141 cggcccatct cgttcatcca ggacctctac acgccgctct acctctcccg gccggtggtc
     1201 cagagcgagc gctatgcctc gctgcgccca caggagtacg cggagggcat ggagactctg
     1261 aaagctcccg catccagcaa ggcgatgcaa cgccgggagg ccagcggcaa gcccccggcc
     1321 gtgatggcgc ctgccccggc atccaccgcc attgcggcag atgcgggcgc ggccaacggt
     1381 gccatgcgct cggaccttca gcccgccgcg accggggaga ctgccggcga actgtttgtc
     1441 taccagatga aggagccggt gacgatcccc cggcggcagt cggcgatgct cccgatcatc
     1501 aaccagaagg tccaggcgcg aaaggtgagc atctacaacc agcgcgccct cgcaaatcac
     1561 ccgctcagcg gcgtctggct caccaacaac accgatctga cccttctggc tggtccggtc
     1621 accctcctcg acggcggcac ctatgccggc gacgccctcc tcggcaacat cacgcccaag
     1681 gagcgccgcc tcctcagcta cgccgtggac ctgaagacga cggtcgattc gtcggtgaag
     1741 agctcgaaga agatcgccag cgcccggatc gttcgcggca cgatgacgat cacccacaag
     1801 aggtacttca cccagaccta ctcgctcaag aacaaggcgg acgaggagcg ggtggtgatc
     1861 atcgagcatc ccttcaatcc ggagcgcacg ctcatcgagc cgaaggagcc cactgagaag
     1921 acgcccgagt tgtatcgctt cgaggtgacc gtgcccgcgg gtgcaacgcg cgcgtttgac
     1981 gtgcgcgaag agcagaccga gatggagatg atggatctcc tccccgcgga cttgggcacg
     2041 cttcagatgt acgcctccag c
//
LOCUS       DAOSCV010000842         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36010_length_2056_cov_3.111444, whole genome shotgun sequence.
ACCESSION   DAOSCV010000842 DAOSCV010000000
VERSION     DAOSCV010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36010_length_2056_cov_3.111444"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..1478)
                     /locus_tag="PLY56_16885"
     CDS             complement(255..1478)
                     /locus_tag="PLY56_16885"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ23204.1"
                     /translation="MASTKATLAIHGGTPVRNTAVRPWPAWPVWDSREEEALLRVLHS
                     GKWGSTSGTEVHAFEEEFARFQDAQYGICNVNGTASLEIALRAAGIAAGDEVIVPPYT
                     FIATASSVLMVNAVPIFADIEPDTYNLDPKAAEAAITPRTRAIIVVHVGGCPVDLDAF
                     TELARKHNLTLIEDCAHAHAASWRGRKVGAIGDMGTFSFQSSKNINAGEGGIILTNDK
                     QLHERCWSIVNVGRIPDGAWYEHHVLGSNYRLTEFQGALLRAQLTRLPEQAARRMENA
                     RFLNAELGRIPGIRPMRLDERVTGHGYHLYLFRYNAAEFGGMPREEFIRTLSAEGIPC
                     SPGYVPLYRTDLFRTQGTVAGAQMDYTKVVCPVAERACTEEAVWFTQNMLLGTREEMQ
                     EIVDAIAKIQAAARA"
     regulatory      1679..1811
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
BASE COUNT          365 a          652 c          653 g          386 t
ORIGIN      
        1 cgaagcaccg gaccgcatat ccactttgaa gtgttgagga acggccgggc gatcaacccg
       61 ctgagcgtcg ccggctaatc tgcccattcg agaagcggtt ctcaggagcc gagcgcactg
      121 ccgaagcact tctcgaatag gcgctaagcg accaatccga aggctcgctc gggaaggatg
      181 aaaaaacctt cccgagcgag ccttcgacca cgtaccgcac caggcatccg gagcggttgc
      241 cccatgatac ggagttacgc gcgtgcggcg gcctggatct tggcaatggc gtcgacgatc
      301 tcctgcatct cctcgcgcgt gcccaggagc atgttctggg tgaaccagac ggcctcctcc
      361 gtgcacgcgc gctcggcgac cgggcagacc accttcgtgt aatccatctg cgctccggcc
      421 acagtgccct gggtgcgaaa gagatccgtg cggtagaggg gcacatagcc ggggctgcac
      481 ggaatcccct cggcggatag cgtgcgaata aactcttcgc ggggcatgcc gccgaactca
      541 gccgcgttgt agcggaacag gtagaggtga tagccgtggc ctgtcacccg ctcatccagg
      601 cgcatcgggc ggattcccgg gattctcccc aactccgcgt tgaggaagcg ggcattttcc
      661 atgcgccgcg ccgcctgttc cggcaggcga gtcaattgcg cgcgtagcag cgccccctga
      721 aactcggtca gacggtagtt cgaacccagg acgtggtgct cgtaccaggc gccatcagga
      781 atgcgaccca cattgacgat cgaccagcac cgttcgtgga gctgcttgtc attcgtgagg
      841 atgatcccgc cctcgccggc gttgatgttc ttgctcgact ggaaggagaa ggtccccata
      901 tcgccgatgg cacccacctt tcgccctcgc catgaggccg cgtgggcgtg cgcacagtcc
      961 tcgatcagcg tgaggttgtg cttgcgcgcc agctcggtga acgcgtctag gtcgaccggg
     1021 cagccgccca catggaccac gatgatcgcg cgcgtgcggg gcgtgatggc ggcctcggcg
     1081 gccttcggat ccagattgta agtatcgggc tcgatatcgg cgaagatcgg caccgcgttg
     1141 accatcaaga cggaactcgc cgtcgcgatg aaggtgtagg gcggcacgat cacctcgtcc
     1201 ccggcagcga tgcctgccgc gcgcagggcg atctcgagcg acgcggtgcc gttgacgttg
     1261 cagatgccgt attgtgcgtc ctggaagcgc gcgaactcct cctcgaaggc gtgcacctcc
     1321 gtgccggaag tgcttcccca ctttccggag tggagaacac ggaggagggc ctcctcttcc
     1381 cgggaatccc agactggcca ggcgggccat gggcggactg ccgtattgcg cacgggagtg
     1441 cccccgtgaa tcgctagcgt tgctttggta gatgccatct tgctccactc ctcgcaggtg
     1501 actacacatc cctctcgcct gccggcgatg ggccaacaca ccccttcgtt tccccccggg
     1561 atgcgcattc tcctctcggt cgtgccaagc cacgatctgg ctttacaaca acgccgttgt
     1621 gcggtataat gatatcagtc gctcgctgcc taggcagcga gggcgataac aaaatagcga
     1681 taccagccgg ccgagtttcc catcagggaa aacgggggac ccaaatgagt ggggcgcacc
     1741 ctgcatctgc agggcgggta ctcttcgccc gagcccgtca gctaacctcg taggcagtac
     1801 gaagagatgc atgcggtagc agcgcacgtc gcgttgaact cggccacggc ctttcgtcgt
     1861 ggctttttgc atttcaggaa tgcgtcaacg cgtggcgcgg ccttcccgtc agcgcctttc
     1921 ggcccggcat tgccgggctt ttttgtttgg ccagcggatc gcttctgcga caggcggtgt
     1981 tcgcgtggct gtggaatcgc cgcggccacg gtggaagtgg tggggcggcc caatagtgag
     2041 gccggctcca cgcgag
//
LOCUS       DAOSCV010000843         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36096_length_2052_cov_3.822233, whole genome shotgun sequence.
ACCESSION   DAOSCV010000843 DAOSCV010000000
VERSION     DAOSCV010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36096_length_2052_cov_3.822233"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLY56_16890"
     CDS             complement(<1..1223)
                     /locus_tag="PLY56_16890"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ23205.1"
                     /translation="MARFLIGATLALLMAAGWAASLESPEIHTLRIAWRAESGIGTQG
                     RVSVSEGKILRLLGWGLRAGDAVGADWSWQCDSGAAGPRSLLVTLSAPRTAAVQVTLG
                     PQTHTFPLADLAKDDRITPLDGPVAVELLPTPRPLSGERESDIPALASSERAGIWCGW
                     IAFDGREDQIFLAQLVDARWESRGALPRARGKIDQLALAATPQGNLIAVWGQERQRNW
                     DLYASVLSGGRWSRPSRVTRNPGPDTNPRLASDTQGRVWLAWQSFEGSFSHIRLAYWE
                     EGRWSPHMLLTDVPANHWAPALAVSPQGTVHVAWDSYASGSYDLYLRSFEGGQLTPIR
                     RITHGPEYEANASLACGPDGRLWIAYDLGEADWGSERHAAELHRERRIGLRCLEGERL
                     LQPPLPPTEALEGIAT"
     gene            1467..>2052
                     /locus_tag="PLY56_16895"
     CDS             1467..>2052
                     /locus_tag="PLY56_16895"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ23206.1"
                     /translation="MNRIVAKDWLAPTVARFEVEAPLTARKRRAGQFVILRVTESGER
                     IPITIAGSNPEAGTITLVVQKVGKTTSDLCALEPGEYLSDLVGPLGQPTHIERWGRVV
                     CVAGGVGAAVILPIALAAQAAGNQVDTILGARSKDLLILEKELAAASERLHITTDDGS
                     RGEKALVVAPLERILQAGPVHQVLAAGPLPMMRAV"
BASE COUNT          342 a          668 c          699 g          343 t
ORIGIN      
        1 gtggcgatgc cctccagggc ttcggtcgga ggcagcggcg gttgaaggag acgctctccc
       61 tccagacagc gcaagccgat gcggcgctct cggtgcagct cggcggcatg gcgctcgctg
      121 ccccaatcgg cttctcccag gtcataggcg atccagagcc ggccatcggg cccgcacgcg
      181 agtgaggcgt ttgcctcata ctccgggccg tgggtgatcc gccggatcgg ggtgagctgt
      241 ccgccctcaa aggagcgcag atagagatcg tagctcccgc tcgcgtagga atcccacgcc
      301 acgtgtaccg tgccttgcgg ggagactgcc agtgccggcg cccagtggtt cgctggcaca
      361 tcggtgagca gcatgtgggg cgaccagcgc ccctcctccc agtatgccag ccggatatgg
      421 gagaaggagc cctcgaacga ttgccaggcc agccacacgc ggccctgggt atcggaggca
      481 agccgggggt tggtatcggg gccggggttg cgtgtcaccc gggaaggcct gctccaacgc
      541 ccgccagaga ggaccgatgc gtagaggtcc cagttccgct gccgctcctg gccccacacg
      601 gcgatcaggt tgccctgggg cgttgctgcg agcgccagct ggtcgatctt ccccctggcg
      661 cgtggcaggg cgcctcgaga ctcccaccgg gcatccacca gctgcgccag gaagatctga
      721 tcctcgcggc catcgaacgc gatccacccg caccagattc ccgcgcgttc gctcgatgcc
      781 agggcaggga tgtcgctctc tcgctctccg gagaggggcc ggggcgttgg cagcagctcg
      841 accgcaaccg gcccatccag tggggtgatc cggtcatcct tcgccaaatc cgcgagaggg
      901 aaagtgtgcg tctgcggtcc tagcgtcacc tgtacggcgg cggtgcgtgg cgcggacagg
      961 gtgaccagca gggagcgagg gcccgcggcg cccgaatcgc attgccagga ccaatcggct
     1021 cccacggcat cgcccgcgcg cagaccccag cccagcagcc gcaggatctt gccctcggag
     1081 accgagacgc gcccctgcgt gccgatcccg ctctcggctc gccacgcaat acggagcgtg
     1141 tggatctctg gcgattccag agatgctgcc cagcccgcgg ccatcaacag cgcgagcgtt
     1201 gccccaatga ggaatctcgc catctccact ccctcccacg cattctgttt ccgaactgca
     1261 agggcggtgc caagcgtgct gtgtgtgggc gcgggtgcgc gggcttggct cctttccggt
     1321 tggcggccga gcaccgggct atcgacgggg aatccgctga tgggggtacc aagaaagtac
     1381 cgatagactc tccttccccg gaatgctata atgaggtaga aatagggatt ctgcgcatca
     1441 tccatgtgag aggggaatga atccttgtga atcgcatcgt tgccaaagac tggctggcgc
     1501 ccaccgtggc gcgttttgaa gtggaagccc ccctgacagc acgcaagcgc cgcgccggcc
     1561 agtttgtgat cctgcgcgtg acggagtcgg gcgagcggat cccgatcacg atcgccggct
     1621 cgaacccgga agcaggaacc atcaccctgg tcgtgcagaa ggtggggaag acgacctcgg
     1681 atctttgtgc cctggaacca ggggagtatt tgagcgatct cgtgggcccg ctgggccagc
     1741 caacgcatat cgagcgctgg gggcgcgtgg tgtgcgtggc tggcggcgtg ggcgccgcgg
     1801 ttatactgcc aattgctctg gcggcgcagg ccgccgggaa tcaagtggac accattcttg
     1861 gcgcgcgttc gaaggacctt ctgatcctcg agaaagagtt ggcggctgcc agcgagcgcc
     1921 tccatattac caccgacgac ggatctcgcg gcgagaaggc cctcgtcgta gcgccgctcg
     1981 agcgcatcct gcaggccggg ccggtgcatc aggttctggc ggcgggcccg ctgccgatga
     2041 tgcgagccgt gt
//
LOCUS       DAOSCV010000844         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36104_length_2052_cov_2.827742, whole genome shotgun sequence.
ACCESSION   DAOSCV010000844 DAOSCV010000000
VERSION     DAOSCV010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36104_length_2052_cov_2.827742"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLY56_16900"
     CDS             complement(<1..238)
                     /locus_tag="PLY56_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23207.1"
                     /translation="MVNPLVTGVREFLARQERAGRTCTIVRQADPEETLHTESEPFDR
                     AVHLVETGALEARDTGYRPGEGGSASGFRFFLDGV"
     gene            443..>2052
                     /locus_tag="PLY56_16905"
     CDS             443..>2052
                     /locus_tag="PLY56_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23208.1"
                     /translation="MHHLCDHTRWACRMALAFFFLLCLAGLGPGRAAPEMADPEVVAE
                     WRFDRPEVLDGLRAVNWERLEIRDGALQGSTRYDSQLVPPPVRIDASRARAIGLRVRS
                     DTSGSGELFFRRENEAFTDSRQIKWSITGDGEWHELYIPIEHPEWRGKIVGLRFDPIN
                     PAARLSIAWLRLLTGIPSNRVPNGGFEEGLTGWALQGKGEVITPGSPGQSALRLAPGS
                     QAVASSLDFSFLGTYRLRLRARGGAGTARLEFRDLDGQPTGTPVTLAVPTAKEWSARS
                     MEFDVPMMAAEATLTLSAGQGAAVEWDGVAVDEIARFPVNIGPEPQLPWESSWIWHPQ
                     SSDNVTAGFRKAFDLPAGKVEHALLLVTADDTFTLFLNGEKVGEDGEQDGWRTPELID
                     IARHLRPGRNVLAVLVREFASAEGLILQGDLRVNGQRIPLKTDASWKVALSPADGWEA
                     PGFDDSGWAAAREVGRPPVAPWGALSMPELGPLAPCSVVRRDVPNTVRAGGRLRVAAT
                     FRTKAGGPVVAELLGDAVSLPLEPAGGWP"
BASE COUNT          290 a          695 c          758 g          309 t
ORIGIN      
        1 gcacgccatc gaggaagaac cggaagcccg aggcgctgcc gccctcgcca gggcgatagc
       61 cggtgtcgcg cgcttccaga gcgccggttt caaccaggtg gacggcgcgg tcaaacggct
      121 ccgattccgt gtggagcgtc tcttccggat ccgcctggcg gacgatggtg caggtgcgcc
      181 cggcgcgctc ctgccgcgcc aggaactcgc gaacgcccgt gaccagcgga ttgaccaacg
      241 cgcgcctccc ttccaatccc ctggcgccag tatagcagga agccaggcac agggtcaagg
      301 agttgcacgc agtgccagga agcccgccgc cgggctgcga acaggtaggg tgaggtcagg
      361 ggccggcggg ggatgcctgc cggccgggcg cgggcgagag tgggaccgcg cgttgggaag
      421 cgacgatcag gcaggagaga ccatgcacca cctttgtgat cacactcgtt gggcgtgccg
      481 gatggcactg gcgttcttct tcctcctctg cctggccggc ctggggccgg gccgggcggc
      541 gccggagatg gcggatccgg aggttgtggc cgagtggcgg ttcgatcggc cagaggtcct
      601 ggacggcctg cgcgcggtga actgggagcg gctggagatt cgtgacggcg ccctccaggg
      661 aagcacccgt tacgactcgc agctcgtgcc cccgccggtg aggatcgacg cctcgcgggc
      721 gcgcgccatc ggcctccgcg tccggtccga cacctccggg agcggcgagc tcttcttccg
      781 tcgggagaac gaggccttca ctgactcgcg ccagatcaag tggagcatca ccggcgatgg
      841 cgagtggcat gagctctata tcccgattga gcacccggag tggcgcggga agatcgtggg
      901 cctccggttc gacccgatca accccgcggc gcgcctctcg atcgcctggt tgcgcctgct
      961 gaccggaatc ccgagcaacc gcgtgccaaa cggtggcttc gaggaggggc tcaccggttg
     1021 ggcgcttcag ggcaagggtg aggtgatcac ccccgggtcg cccgggcaga gcgcactgcg
     1081 cctggcgcct gggagccagg ccgtggcgtc ttccctcgac ttctccttcc tcggcaccta
     1141 ccgcctgcgc ctgcgcgctc gcggcggcgc gggtactgcc cgcctcgagt tccgcgacct
     1201 ggatgggcag ccgacgggca cgccggtgac gctcgccgtg cccaccgcca aggagtggtc
     1261 ggcgcgttca atggagtttg acgtgccgat gatggccgcg gaggcgacgc tgacgctttc
     1321 ggcggggcag ggtgcggcgg tggagtggga tggcgtggcg gtcgacgaga ttgcccgctt
     1381 cccggtgaac atcgggcccg agccacagct tccctgggaa tcgagctgga tctggcaccc
     1441 gcaaagcagc gacaacgtga ccgccggatt ccgcaaggcg ttcgacctcc ccgccggaaa
     1501 ggtggagcac gcgctcctcc tcgtcaccgc ggacgacacc ttcacgctct tcctcaacgg
     1561 cgagaaggtg ggcgaagatg gcgagcagga tggctggcgc accccggagc tgatcgatat
     1621 cgcccggcat ttgcgccccg ggcgcaacgt cctcgctgtc ctggtgcgcg agttcgcgtc
     1681 cgccgagggc ctgatcctcc agggcgatct ccgcgtgaat ggccagcgga tccccctcaa
     1741 gaccgacgcc tcctggaagg tggcgctctc gccggcggat gggtgggaag cccccgggtt
     1801 tgacgactcc gggtgggcgg cggcgcgcga ggtgggccgg ccccccgtgg cgccctgggg
     1861 tgccctgtca atgccggagc tcggcccgct ggcgccgtgc tcggtcgtcc ggcgcgacgt
     1921 gcccaacacc gtgcgcgcgg gcgggcggct ccgcgtcgcc gccaccttcc gcacaaaggc
     1981 aggtggcccg gtggtcgccg agctcctggg agacgcggtg agcttgccgc tggagcccgc
     2041 gggcgggtgg cc
//
LOCUS       DAOSCV010000845         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36171_length_2049_cov_2.970411, whole genome shotgun sequence.
ACCESSION   DAOSCV010000845 DAOSCV010000000
VERSION     DAOSCV010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36171_length_2049_cov_2.970411"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLY56_16910"
     CDS             <1..387
                     /locus_tag="PLY56_16910"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ23209.1"
                     /translation="IYCERRQGDLAAPLVDALAALPKDEAPEDMAEILLNTAIAYGSR
                     GNNDRALVYLAKVIQEYPGTEEAEWAGFFTGLALGLKGEREAAIKQLEQVISTAKNEG
                     IRERARILRDRFRAEDEQGPPTGPSL"
     gene            complement(494..1510)
                     /gene="thiL"
                     /locus_tag="PLY56_16915"
     CDS             complement(494..1510)
                     /gene="thiL"
                     /locus_tag="PLY56_16915"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOJ23210.1"
                     /translation="MQLSEVGEFGLIHRLSAIIGGRGGDPSGVFLGLGDDAAGFQPPE
                     NRHLVATADMLVEEIHFRRSWLSSRALGWKALAVNLSDVAAMGGEPHFALVCIAVDSP
                     TSVEYVEGIYAGMQSLAERFGVRIAGGDTVRSRAGLTLSITVVGSVEPERLATRAGAR
                     PGDLLVVTGTLGDSAAGCMALLAGPRAAEKVPPSVLEAHRLPQPRIAEARAAVATGAV
                     TALMDISDGLAGDLHHICEESGVGAELDAGAIPLSEACRDAAARLRLDPLQLALTGGE
                     DYELLLAVSPERVDEVRAAITEATGTRTSVVGQIQPAGCQLLLPNGTRVPLSGAGYQH
                     FPSE"
     gene            1776..>2049
                     /locus_tag="PLY56_16920"
     CDS             1776..>2049
                     /locus_tag="PLY56_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23211.1"
                     /translation="MSEGDGGPRLVMPAPAKVNLVLCIGGTREDGFHDLVSIFQAVEI
                     RDTVILSTLDAPEIRVTASDVRLPCDERNTAYRAARLLRDAHAPDRG"
BASE COUNT          356 a          702 c          685 g          306 t
ORIGIN      
        1 atctactgtg agcgacggca aggcgatctg gcggcgcccc tcgtggatgc gctcgcggcg
       61 ctgcccaagg acgaagcccc cgaggatatg gccgagatcc tgctcaacac cgcaatcgcc
      121 tatggctctc gtggcaataa cgaccgggcg ctcgtgtacc tcgccaaagt catccaggag
      181 tatccgggca ccgaggaagc cgaatgggcc ggcttcttca ctggcctggc gctaggtctg
      241 aagggcgagc gcgaggcggc gatcaagcaa ctggagcagg tgatctccac cgccaagaac
      301 gaggggatcc gcgagcgcgc caggatcttg cgcgatcgct ttcgggcaga ggacgagcag
      361 ggaccaccca cggggccgtc tctctagcat tcacacgcga gcgagcaagc gatacgcaac
      421 atcggaccag cacgcggcgg ggtatgacga ccccgccgtt gtgcctggcc ggtagcgcca
      481 ggccctccct gggctactcg ctcggaaagt gctggtaacc tgcccccgac aagggcaccc
      541 gcgtgccatt cgggagaagc aactgacatc ccgctggctg aatctgcccg accacgctcg
      601 tccgagtgcc agttgcctcg gtaatggcgg cgcgcacctc atcgacacgc tcgggtgaga
      661 ccgcgagcag caactcataa tcctccccgc cggtgagcgc cagctggagc ggatcgaggc
      721 gcaggcgagc cgcggcatcg cggcatgcct ccgagagggg gatcgcgcct gcatccaatt
      781 cggcgcccac gccgctctcc tcgcagatgt ggtgcagatc gccggcgagg ccatcgctga
      841 tatccatgag cgcggtgacc gctcccgtgg ctaccgcggc gcgcgcctcg gcgatccgcg
      901 gctgtgggag acgatgtgcc tccagcacgg aggggggaac cttctccgct gctcgtggcc
      961 ccgccaggag cgccatgcat ccggcggcgg agtcgcccag ggtacctgtg accacgagca
     1021 gatcccctgg acgggcgccc gcgcgggtgg ccagacgctc cggctccacg ctgccgacga
     1081 cggtgatcga gagggtgagc ccggcgcgcg agcgcacagt gtcgcctccg gcgatgcgca
     1141 cgccaaagcg ctctgccagg ctctgcatcc ccgcgtagat cccctcaaca tactccacgg
     1201 aagtgggcga gtccaccgcg atgcagacga gggcgaagtg tggctcgccg cccattgcgg
     1261 ccacgtcgct cagattcacc gccagcgcct tccaccccag ggcccgcgac gagagccagc
     1321 tgcgccggaa gtgtatctcc tcgacgagca tgtcggcggt ggctacgagg tgccggtttt
     1381 cggggggctg gaaccccgcg gcatcatcgc cgagacccag gaacaccccg gatgggtcgc
     1441 caccccggcc ccctatgatc gccgagagcc ggtgtatcaa cccgaactcg cctacttccg
     1501 aaagctgcat cctctctccc ctcgctaagc ctgcgcaatg gcggaatccc tggggccggc
     1561 cccatcgcgc ggagcgaggc cttcccttgg ctactttggc gcccgaacac tctgattcct
     1621 tgcgaatctg ccaggaacca ggccggacaa cgcggaatct ctttgttgga actgcgccat
     1681 gcctgtatgg tggcatcggg caggagcggc ggcgcgccgg acaccgcgtc tggacgggcg
     1741 ttgcagctat gggagtggag aggagagata cggaggtgag cgagggcgat ggcgggccgc
     1801 gcctggtgat gccggcaccc gcgaaggtga acctggtgct atgcatcggc ggaacgcgcg
     1861 aggacggctt ccacgacctc gtctctatct tccaggcggt ggagattcga gacaccgtga
     1921 tactctccac actggacgcg cccgagatcc gcgtgaccgc gtcggatgtg cgcctgccgt
     1981 gcgacgagcg aaacacggcg tatcgcgccg cccgcctgct tcgcgacgcg cacgcgccgg
     2041 accggggcg
//
LOCUS       DAOSCV010000846         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36190_length_2048_cov_2.532363, whole genome shotgun sequence.
ACCESSION   DAOSCV010000846 DAOSCV010000000
VERSION     DAOSCV010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36190_length_2048_cov_2.532363"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1015
                     /locus_tag="PLY56_16925"
     CDS             182..1015
                     /locus_tag="PLY56_16925"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ23212.1"
                     /translation="MRLLSNRDEDERWPQQLLRRRLLATAVLILLFGLGLLAWARLYP
                     APAALLEGEWRLRAGDIRGADSAFQRFLLLNQESADSFVIVASRYIAAERWDAAARSL
                     ERAVQTDPSHRARVDLGIVYEHLGRDDEAEAAYREAIRHRPDDAYALNALGYFYAQRA
                     MHLDEALALLHRALELLPNDGSIMDSLGWALYQRGDVVAALRQLAASVERVPSFGEVR
                     LHLGVALARRGARDQALVELGKAVMLDPELPGAREALETVRRGGLPPLPFPYEPPARR
                     R"
     gene            complement(1123..>1397)
                     /locus_tag="PLY56_16930"
     CDS             complement(1123..>1397)
                     /locus_tag="PLY56_16930"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOJ23213.1"
                     /translation="QKKYAGKPVKVIGVNTDKQGKALRNWMKARNLTFTVVADTNHKV
                     SGQYQVEAFPTLYVIDKKGIVREHILGFDPEMETNLGRLIDNLLKK"
     assembly_gap    1398..1407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1408..1660)
                     /locus_tag="PLY56_16935"
     CDS             complement(<1408..1660)
                     /locus_tag="PLY56_16935"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ23214.1"
                     /translation="MAIAILGLGGALLVPALRAGTSGSRALVGQANADSASITVGRRA
                     PDFTLRDPEGKQVRLKDLKDKVVVLSFWATWCPPCRMEMP"
     gene            complement(1808..>2048)
                     /locus_tag="PLY56_16940"
     CDS             complement(1808..>2048)
                     /locus_tag="PLY56_16940"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ23215.1"
                     /translation="WRCRYVANQGGIAVKGGWAGPYSDHTAFARAGVPVVYYHRSPHP
                     LTHTPRDTPATIRPEYLRLAVLAVSPAIEAICNRP"
BASE COUNT          336 a          664 c          659 g          379 t
ORIGIN      
        1 cgatccgccg cgcatccttc gccagggggc tctcccggcc gctgagatac gcaagtgggt
       61 gccggacctc gttgagtaga gagacacggt gagaatgccg cttccgatct ggccgggggc
      121 tctcaccgtc gccctctccg tgcgtgcggg ggagaaggat gggaagccgg gagccgtgag
      181 aatgcgtctg ctgtccaatc gcgacgagga tgagcgttgg ccgcagcaac ttctgagaag
      241 acgcctgctc gccaccgctg tgctgatcct gctctttggc ctgggtcttc tggcgtgggc
      301 acgcctctat cccgccccgg cggctctcct ggagggcgaa tggcggctgc gcgcgggcga
      361 tatccgaggg gccgactctg cgtttcaacg cttccttctc ctgaatcagg agagcgcaga
      421 cagtttcgtg atcgtcgcgt cgcgctacat cgccgcggaa cgttgggatg cagccgcccg
      481 ctcgctcgag cgcgccgtgc agaccgaccc atcccaccgc gcccgggtgg atctgggtat
      541 cgtctatgag caccttggtc gtgacgacga ggccgaggcc gcctaccgcg aggcgatacg
      601 ccaccgcccc gacgacgcct acgcgctcaa tgcgctcggc tatttctacg cacagcgcgc
      661 gatgcatctc gatgaggccc tggcgctact tcaccgggcg cttgagctcc tgcccaacga
      721 cggcagcatc atggacagcc tgggatgggc gctttaccag cggggcgatg tggtcgcggc
      781 gctccggcaa ttggcggcat ccgtcgagcg cgtgccgtcc ttcggggagg tgcgcctcca
      841 tctgggagtg gccttggcgc ggcgaggcgc gcgtgaccag gcgctcgtcg aactgggcaa
      901 agctgtgatg ctcgatccgg agcttccagg ggcgcgcgag gcgttggaga ccgtgcgcag
      961 gggaggcctt ccacctctcc cttttccgta cgagccacct gcgcggagaa ggtgagccgc
     1021 actggggggc ggcggctggg ggacacgcgt ccgggtgcag gctctccctg ggagcggaga
     1081 gagggcatat ccgcatggcc gctcccaggg agatcgtgac ccttacttct tcagtaggtt
     1141 atcaatgagc cggccgaggt tcgtctccat ctcaggatca aaccccagga tgtgctcgcg
     1201 cacgatgccc ttcttgtcga tcacatagag cgtggggaag gcctccacct gatactgccc
     1261 ggataccttg tggttcgtat cagcgactac cgtgaaggtg agattccggg ccttcatcca
     1321 gttccgcagc gccttgcctt gcttgtcggt gttcacgccg atcactttga ctggcttgcc
     1381 cgcgtacttc ttctgaannn nnnnnnngag gcatctccat gcggcatggc gggcaccacg
     1441 ttgcccagaa gctgagcacc accaccttgt ccttcaaatc cttcagccga acctgcttcc
     1501 cctccgggtc tcgcagggta aagtcgggag cccgccggcc gaccgtaatc gatgcggaat
     1561 cggcattcgc ctggcctacc agggcgcgcg acccagacgt gcccgcgcgg agcgcaggca
     1621 ccagaagtgc ccctccgagg ccaagaatcg caattgccac ccacctcttc ttcaccatat
     1681 ccatcccatc ccttccaaga gcacggagca ggatctgcgc gctctctgta ttagagaccg
     1741 tgcagcaccg gcaggattcg ccagattcgt tacctaaagg cccatccgta ttcaggacaa
     1801 cgacgagcta cggacggttg cagatcgcct caatggcggg agagacggcg aggactgcca
     1861 gcctcagata ttccggccgg atggttgccg gggtgtcgcg cggggtgtgg gtgaggggat
     1921 gcgggcttcg gtggtagtac accaccggaa ccccggcgcg tgcgaacgcg gtgtgatcgc
     1981 tgtacgggcc ggcccagccc cccttcaccg cgattccgcc ttgattcgcc acatagcggc
     2041 agcgccat
//
LOCUS       DAOSCV010000847         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36213_length_2047_cov_3.525602, whole genome shotgun sequence.
ACCESSION   DAOSCV010000847 DAOSCV010000000
VERSION     DAOSCV010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36213_length_2047_cov_3.525602"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLY56_16945"
     CDS             complement(<1..399)
                     /locus_tag="PLY56_16945"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper amine oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HOJ23216.1"
                     /translation="MKVRLGELFGQARVLPALLGAGSVLGTLALAALLPAPCAAAREY
                     RISVDGQPVQFDGMSPVQVSGRVLVPLRGVLERIGAKVEWSASSKTVVAQRGSVRLEL
                     PVGQRSAAVNGRSVTLDVPAMIMRGNVMVPL"
     gene            complement(696..1604)
                     /locus_tag="PLY56_16950"
     CDS             complement(696..1604)
                     /locus_tag="PLY56_16950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ23217.1"
                     /translation="MGAGGQITYLWRFLTAVYREYMRDDCRLMAAAISFYALLSLVPL
                     LLVGLSTFGYVLGNDRAFDAVYAFLIQLLPQDLAATLLPEEVAGAGLRSYLKKTLVKP
                     AGVAGIVGVVSWIWAGTNCFVVITAALDVAWETGKRRGFIASRLIAVGMMMLVATLAL
                     LSFGITQVLKIIREYPIPWLGIRSGEIPFLWQLVGWAVPATLSILVFVLCYLILPDRR
                     IGWRPALIGGVAAGMLWELAKQGFAFYVANFVDYNSIYGSLGGLVILVMWVYLSAVML
                     LLGAEVASVSADWSAKSVQSPAAEDR"
     gene            complement(1768..>2047)
                     /locus_tag="PLY56_16955"
     CDS             complement(1768..>2047)
                     /locus_tag="PLY56_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23218.1"
                     /translation="PNSAPAKVTPLQDGSAGAIAEVDGIVHVLLCGQAGVARRVEAAG
                     KVFETDAEVAVVSLKSTGPEAFLVRGKKLLVDGKPVAPAQGEWRLRRE"
BASE COUNT          398 a          732 c          601 g          316 t
ORIGIN      
        1 caggggcacc atgacgttgc cccgcatgat catcgcgggc acatcgagcg tcaccgagcg
       61 gccattcacg gcggcgctcc gctgccccac gggaagctca aggcgcactg agccccgctg
      121 cgcgaccacc gtcttcgaag acgcggacca ctccaccttc gcgccgatac gctccaggac
      181 ccctcgcaac ggcacgagca cgcgcccgga tacctgaacc ggactcatcc catcaaactg
      241 aacaggctgg ccatccacgc taatgcgata ctctcgcgct gccgcgcagg gggccggcag
      301 gagggctgcc agcgcgagcg ttccgagcac acttcctgcc ccaagtaggg ccggcaagac
      361 gcgcgcctgt ccgaagagtt caccaagacg tactttcata gggcttcggg ggtgcggcgg
      421 ccgatagccc ccccggcctc ccggcggggg agggatagcc ccctgcaatt tctcgcgttt
      481 catggcccct ccgttcgcgg tataatcatg gtgtaaggca ctccacttcg cagagcaacg
      541 gtttcatctg ccctggtgct tgcttcctga agcccctagg ggtgccggtt cgcgtggaag
      601 aatctccacg cggcattgat gtttcaacac cgggctctgc cgatgggcgg cctggccgta
      661 cccgccagac ccgccactgc gcggcagcgg caggcctaac gatcctccgc cgctggggac
      721 tggacggatt tggcagacca atcggcgctg acgctggcga cctctgctcc aagaaggagc
      781 atgacggcag acaggtagac ccacatcacc aggatgacca agccaccaag agagccgtag
      841 atactgttgt agtcaacgaa gttcgcgacg tagaacgcaa acccctgctt cgcaagctcc
      901 caaagcatcc ccgcggccac gccgccgatg agcgccggac gccaaccgat gcgcctatct
      961 ggcaggatca ggtagcagag cacgaacacc aggatcgaga gcgttgccgg caccgcccac
     1021 ccgaccagct gccataggaa cgggatctct ccagagcgga ttcccagcca tgggatgggg
     1081 tattcccgga tgatcttcag aacttgcgtg atcccgaagg agaggagcgc cagcgtggca
     1141 accagcatca tcatgcccac ggcgatcaga cgactcgcga taaagccccg gcgctttccc
     1201 gtttcccagg ccacatcaag cgcggcggtg atcaccacaa agcagttggt tcctgcccag
     1261 atccagctga ccacgcccac gatgccagcc acccccgccg gtttgaccag cgttttcttc
     1321 agatagctgc gcaggcccgc gccagccacc tcctccggga gcagcgtcgc agccaagtcc
     1381 tgcggcagaa gctggatcag gaaggcgtag accgcgtcaa atgcccggtc gttgccgagc
     1441 acatacccaa aggtggaaag accaacgagg agcagcggca cgagcgagag cagggcataa
     1501 aaggagatgg ctgcggccat cagccggcaa tcatcacgca tatactcgcg gtagaccgct
     1561 gttagaaacc gccagagata tgtgatctgt cctccggcac ccactcagca cccccttgcc
     1621 ggaggccgtg cctccgtggc ccattatagg gaggctgctc ccgattgtca accctacgca
     1681 cgtctgccgg gcctgggagc cgggccgtcg gcgcctccca tcccgcgccc ggtcccttca
     1741 cccgtggcag cacccctcgc ctggcggtca ctcccggcgg agccgccatt ctccctgtgc
     1801 gggcgcgacc ggcttcccat ccacgagcaa cttcttaccg cgcaccagga aggcctccgg
     1861 accggtggac ttcaggctga ccacggccac ctcggcatcc gtctcgaaca ccttgccagc
     1921 ggcctccacg cggcgtgcga cgcccgcttg tccacacagg agcacgtgga cgatcccatc
     1981 cacctcggcg atggcccccg cgctcccgtc ttgcagcggc gtcaccttgg ccggcgcgga
     2041 gttgggg
//
LOCUS       DAOSCV010000848         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36435_length_2038_cov_2.468986, whole genome shotgun sequence.
ACCESSION   DAOSCV010000848 DAOSCV010000000
VERSION     DAOSCV010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36435_length_2038_cov_2.468986"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PLY56_16960"
     CDS             complement(<1..1495)
                     /locus_tag="PLY56_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23219.1"
                     /translation="MRKILLLSVIAVALASVPGWCAQGVSRQDLGRTAKLTILVDKVM
                     QPVEGWVTKEWMVKAAADAGFNVFSPRRGHERLDEVRQVTEWCQKYGIYHMPWMRGSL
                     EAPKGPEADGKRVVWASGNEQPLWSPNSDEFWEWTARYIIEYAKISANNKHLMGVFLD
                     YENYAPGKEGNLYSLSYDDGILKRFAQTRGIEVPKLELAARKSWLEEQKLHEEFERFQ
                     IAHWRERCRVLRQAVDRHDPTFQFCIYPGPGTPFMLEAALPEWSTAQAPIILADPWIY
                     GRPSRFLPQAEALEANRRILQRGIETAKAKGVPFLYAGGIDPVVPGADPEFSGKNAVM
                     SSETTDGYWIFYEGPTYTKQDHADYWKWFTWANRAIAAGRFNAWREPRETEEDWSLTV
                     FKDPEKVGKLVLPQVTGKRVKFPEVTLRGENLLALAAKAGQPVELTLQNVLVGKFPSP
                     IVWELRDASMRKLSSGRIPGQKEGVVRFTPEKDGVYLLGASAGSSAYR"
     gene            complement(1654..>2038)
                     /locus_tag="PLY56_16965"
     CDS             complement(1654..>2038)
                     /locus_tag="PLY56_16965"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23220.1"
                     /translation="GPFDVDDLAAALIRFENGASLVIQVSWAANTSQGSEIRVMGDKG
                     GLEIQPGTSVKVITEDNGFIADIAPQYNKQDAYAAQHAHLIECIRDPEKCLRTDGRQG
                     VVLQHMLDAIYRSAATGKEALIEIA"
BASE COUNT          334 a          659 c          670 g          375 t
ORIGIN      
        1 cccggtaggc gctgcttccg gcggaggcgc ccaacaggta gacgccatcc ttctcggggg
       61 tgaagcgcac caccccttcc ttctggcccg ggatcctccc cgaggagagc ttccgcatcg
      121 aagcatcgcg caattcccag acgatgggag acgggaactt gcccacgagc acgttctgca
      181 gggtgagttc caccggctgc cctgcctttg ccgccagggc aaggaggttc tcgccgcgga
      241 gggtgacctc ggggaacttc acgcgcttgc cggtgacctg cggcaggacc agcttgccga
      301 ccttttcagg atccttgaag acggtgagcg accagtcctc ttcggtctcg cggggctcac
      361 gccaggcgtt gaagcggccg gcggcgatgg cacggttggc ccaggtgaac cacttccagt
      421 aatcggcgtg gtcctgcttg gtataggtag ggccttcgta gaagatccag tagccgtcgg
      481 tggtctcgga gctcatcacg gcgttcttgc cggagaactc cggatcggcg ccgggcacca
      541 ccggatcgat gcccccggcg tagaggaagg gaacgccctt ggccttcgcg gtctcgatgc
      601 cgcgctgcaa gatgcgccgg ttagcttcca gcgcctccgc ctgggggagg aagcgcgagg
      661 gccggccgta gatccagggg tcggccagga tgatcggcgc ctgcgcggtc gaccattcag
      721 ggagggccgc ttccagcatg aagggggtgc cgggcccggg atagatgcag aactggaacg
      781 tggggtcgtg gcgatccacg gcttggcgca gcacgcgaca ccgctcgcgc cagtgcgcga
      841 tctggaagcg ctcgaactcc tcgtgcagct tctgctcctc cagccagctc ttgcgcgctg
      901 ccagctccag cttcggtacc tcaatccctc gggtctgggc aaaccgcttt agaatgccat
      961 catcatagga gagagagtag agattcccct ccttcccggg ggcgtagttc tcatagtcga
     1021 ggaagacacc catcaggtgc ttattgttgg cgctgatctt cgcgtattca atgatgtagc
     1081 gcgccgtcca ctcccagaat tcatccgagt tggggctcca gagaggctgc tcgttcccac
     1141 tagcccacac gacgcgcttg ccgtctgcct cggggccttt gggcgcctcc agtgagccgc
     1201 gcatccaggg catatggtag atgccgtact tctggcacca ctcggtgacc tggcgtacct
     1261 cgtccaggcg ttcatggcca cggcggggcg agaagacgtt gaagcccgca tcggcggcgg
     1321 ccttgaccat ccactccttg gtcacccagc cctccacggg ctgcatcacc ttatcaacga
     1381 gaatggtgag cttggcggtg cgccccagat cttgccggga gacgccttgg gcgcaccacc
     1441 ccggcacgct ggccagggcc acggcgatca cgctgagcag aaggatcttg cgcatcttcc
     1501 cctccttcac acgtgcgggg agtgccgggg atggggcctc cccggcactc cgggcaatgg
     1561 atcaggagag cctgcacgcc cagaatgggc gcgcctcccc acatggactc tctggcctgc
     1621 cgcgtcccac gcggacgtcg ctatgggagg agatcaggcg atctcaatca gcgcctcctt
     1681 gccggtcgcg gcggagcggt agatggcgtc gagcatgtgc tgcaggacga ctccctggcg
     1741 accgtcggtg cggaggcact tctcggggtc gcggatgcac tcgatgaggt gcgcgtgctg
     1801 ggcggcatag gcatcctgct tgttgtactg cggggcgata tccgcgatga agccgttatc
     1861 ctcggtgatc accttgacgg aggttcccgg ctggatctcg agcccgccct tgtcgcccat
     1921 cacgcggatc tcgctcccct gcgaggtgtt ggccgcccac gagacctgga tcacgagcga
     1981 ggcaccgttt tcgaaccgga tgagggcagc ggccaggtca tccacatcga aggggccg
//
LOCUS       DAOSCV010000849         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36548_length_2034_cov_3.357756, whole genome shotgun sequence.
ACCESSION   DAOSCV010000849 DAOSCV010000000
VERSION     DAOSCV010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36548_length_2034_cov_3.357756"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLY56_16970"
     CDS             <1..583
                     /locus_tag="PLY56_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ23221.1"
                     /translation="RLVAMHGGRVEARSEGLGRGSEFVVWLPLAEVETEHHARRSEPR
                     PPLTPRRILIADDNADAAEMLAALLEEEGHWVFTAEDGPSAIGRALSEKPDVILLDIG
                     MPGMDGYTVAHRLRLEPALEGTMLVAVTGYGQDEDRERAFAAGFEHHLVKPVDLDALR
                     RVIAASPTPAWSALSDPAWKDLPVCGEPNLPWC"
     gene            complement(1288..2001)
                     /gene="ric"
                     /locus_tag="PLY56_16975"
     CDS             complement(1288..2001)
                     /gene="ric"
                     /locus_tag="PLY56_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HOJ23222.1"
                     /translation="MAEPDLTPETKVSEVAAHYPAVLRTLEALGIDYCCGGNRTLAEA
                     SQSAGMPVERVVAVLKAAITQAAGTPESEGRWVHAPLDELMQHIVQPHHVYTRAELPR
                     LQEMLALVNQVHGEKYGDLLMPLQEKFLLLKEELEAHLRKEEEVIFPAIAALLAGRTN
                     GSIAQGIEELESEHDGAGDLLKAMRQITDDYQVPEGVCATYEALYRGLQELEADLHRH
                     IHLENNVLFPRALELRRAA"
BASE COUNT          304 a          689 c          687 g          354 t
ORIGIN      
        1 gcggctcgtg gcgatgcacg gcgggcgagt cgaggcccgt agcgaggggc ttggccgagg
       61 gagcgaattc gtcgtgtggt tgcccctggc cgaggtagag acggagcacc acgcccggcg
      121 ttctgagcca cgaccgccgc tcacgccgcg gcgcatcttg atcgccgatg acaacgcgga
      181 tgctgctgag atgctcgccg ccctcctgga agaggaggga cattgggtat tcactgccga
      241 agacgggcca agcgccatcg gccgcgcgct cagcgagaag cctgatgtga tcctgctgga
      301 tatcggcatg cccggaatgg atggctacac cgttgcccat cgcctgcgcc tggagccggc
      361 gctcgagggt acgatgctgg tggccgtcac gggctatggg caagatgagg atcgagagcg
      421 ggcgttcgcg gccggcttcg agcaccacct ggtgaaaccg gtggacctgg atgccctccg
      481 gcgcgttatc gccgcgagcc cgacccctgc ctggagtgcc ctatcggacc ccgcctggaa
      541 ggatctcccg gtgtgtggcg agccgaatct gccgtggtgt tgatagccgc tcccacgctg
      601 tgacgaccga actgggtcac ccccgggcga tgcccggcac cggaactctc cgcgcgtgag
      661 gcgggagaga gccggttgag gccgagcgac tggcgctcgc actctggcgc gccccgcagg
      721 aagcccgtgc ctgtttcgcc aggatgcagg cgctcctgag ccatggcctc agagcctggc
      781 ggcgctcaga cggggtgatc tagtgctcga acggcaagcg tgtgttgcgg ttggcgctct
      841 catcgggcgc cggcgcaatc tcgcacccgg atgtgccctc ctccgttccc tcggtagcag
      901 ccggcacttc cgactcggcg gtggcaaggg gcgccgcccc gggtgccagg ccgccccctc
      961 aaccaggggt ttgccgcacg gcgctggaat ggtccgttcg tctcggcgcg tgctgggcga
     1021 gtgtttcgcg gacaacgctg cccgcgagcc atccagcgaa caggagcagc gccacgatca
     1081 gcgccagcaa cgcccgccgc caggcgaaca gacgccgtct gctgctctca tcgggtgtct
     1141 ccacttcggt cgcccctccc ggcgcggcat aggcggatcc tctggccgcg cccacatagt
     1201 gagggacgcg caagttgggc acccggtttc ctttggagcc agctccattg cgccggacgc
     1261 ggcacccctc ggcatttagg tgcgtgccta cgccgcgcgc cgcagctcca gggctcgagg
     1321 aaagagcaca ttgttctcga ggtggatgtg acggtggaga tccgcctcaa gctcctggag
     1381 cccccgatag agggcttcgt aggtggcaca caccccctcg ggcacctggt agtcgtcggt
     1441 gatctgacgc atcgccttga ggaggtcacc ggctccatcg tgctcgctct ccagctcctc
     1501 gattccctgg gcgatcgatc cgttcgtcct gccggccagc aaagcggcga tggcggggaa
     1561 gatcacctct tcctccttgc gcaggtgcgc ctcgagctct tccttaagca gaaggaactt
     1621 ctcctgcagc ggcatgagca gatcgccgta tttctctcca tggacctggt tgaccagggc
     1681 gagcatctcc tgcaggcgtg gcagctcggc gcgggtgtac acatggtggg gctgtacaat
     1741 atgctgcatc agctcgtcga gcggtgcgtg tacccagcgg ccctcgctct ccggcgtgcc
     1801 ggccgcctgg gtgatcgcgg ccttgagcac ggcaaccacc ctctcgaccg gcattccggc
     1861 actctgcgac gcctccgcca gggtgcggtt gccgccgcag cagtagtcga tgccgagtgc
     1921 ctccagcgtg cgcagcacgg cagggtagtg cgctgcgacc tcgctcacct tggtctcggg
     1981 ggtcagatca ggctcagcca tcccggcctc ctctcgctca tcagggcgcc gcga
//
LOCUS       DAOSCV010000850         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36693_length_2029_cov_3.157042, whole genome shotgun sequence.
ACCESSION   DAOSCV010000850 DAOSCV010000000
VERSION     DAOSCV010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36693_length_2029_cov_3.157042"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PLY56_16980"
     CDS             complement(<1..637)
                     /locus_tag="PLY56_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ23223.1"
                     /translation="MLLFTLSLPAAAGLWEGAREVQIPSSLDGELQGAFFYVPPEAEP
                     GGAPAPLMVALHTWSADYRQAASAAYLAACKRRGWVLIHPNFRGPNHRPEACASDLAV
                     QDVLDAVAYARTHASVDPKRIYLCGASGGGFMSLVMAHRAPDVWAGVSAWVPISDLAA
                     WHRHCTAQKLGYAQDIERSCGGSPSQETEAEYRRRSPLFHLAAARGVPIDLN"
     gene            810..1667
                     /locus_tag="PLY56_16985"
     CDS             810..1667
                     /locus_tag="PLY56_16985"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOJ23224.1"
                     /translation="MEPELIADYQCVCGEGPLWHPMEKRLYWTDIPTGRMFRYDPVTG
                     THEQFYQGEVVGGFTIQADGSLLLFMARGAVKQWRDGTLTTVVEEIPAERESRFNDVI
                     ADPMGRVYCGTMPTPSGPGRLYLLDTDGSISLLLEGVGCSNGLGFTPDRMGLYHTDTR
                     KREITLYDYDAATGAITRPRVAIRTPEGEGAPDGMTVDAEGFIWTAKWGGGCAVRYAP
                     DGTEDRRIHFPARKVSCVTFGGEGYDEMYVTTAGGDQKETDGAGAGALFRVRLGIRGV
                     PEFLSRVCL"
     gene            1786..>2029
                     /locus_tag="PLY56_16990"
     CDS             1786..>2029
                     /locus_tag="PLY56_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HOJ23225.1"
                     /translation="MGTGLRVERDYSLRECNTFGVEAKAAYYARVTSREELAALLQDN
                     PVRDREKLVLGGGSNILFTRDFDGVVIHNAIGGIAVV"
BASE COUNT          351 a          620 c          731 g          327 t
ORIGIN      
        1 cgttcaggtc aatcggcacg ccgcgtgccg ccgccagatg gaaaagcgga gagcgccggc
       61 ggtattctgc ctcggtttcc tggctcggcg aacccccgca cgagcgctcg atatcctgag
      121 cgtagcccag tttctgcgcg gtgcagtggc ggtgccaggc cgcgagatcg gagatcggca
      181 cccaggcgga gaccccggcc catacgtccg gtgcacggtg ggccatcaca agcgacatga
      241 atccgccgcc ggaggcaccg cagagataga tccgcttcgg atcgacggag gcatgcgtgc
      301 gggcgtaggc gacggcatcg agcacgtcct ggaccgccag gtcggaggca catgcctccg
      361 gacggtggtt gggtccgcgg aagttggggt ggatcagcac ccaaccgcgg cgcttgcacg
      421 ccgccaggta ggcagcgctc gccgcctggc gataatcggc gctccaggtg tgcaaggcta
      481 ccattagggg cgcgggcgcg cctcccggct cggcttcggg gggcacgtag aagaacgccc
      541 cctgaagctc gccatcgaga gagctgggga tctgcacctc acgggcgcct tcccaaagac
      601 cggccgcggc cggcagagag agtgtgaata gcagcatggc agaatcctca tcggcagtcc
      661 agttggaccg cccctgccca tttcgcgctc cccctgcggg aaacctgccg gttgccgtac
      721 cgcaggatct gagggcgccg gcagcaaacc tacggagcga gtggcaggca cggccgccgt
      781 tgggcgaccg tgtcagaaag gggacaagcg tggaaccgga gctgatcgcg gattaccagt
      841 gcgtgtgtgg cgaggggccg ctgtggcacc ccatggagaa gcggctctac tggaccgaca
      901 ttcccaccgg acgcatgttt cggtatgacc cggtcacggg cactcacgaa cagttctacc
      961 agggggaggt ggtcggcggc ttcacgattc aggcggatgg ctcgctgctc ctcttcatgg
     1021 cgcgcggcgc cgtgaagcaa tggcgcgacg gcacgttgac cacggtggtc gaggagatcc
     1081 cggcggagcg cgagtcgcgc ttcaacgatg tgatcgctga tccgatgggg cgcgtttact
     1141 gcgggacgat gcccaccccg agcggcccgg gccggctcta cctcctcgat acggatggct
     1201 ccatctcgct gctcctggaa ggcgtcggtt gctcgaatgg cctcgggttc actccggatc
     1261 ggatgggcct ctaccacacc gacacgcgca agcgcgagat caccctctac gactatgatg
     1321 ccgctaccgg cgccatcacc cggccgcgcg tggcgatccg caccccggaa ggcgagggcg
     1381 ccccagacgg catgacggtc gatgccgagg ggttcatctg gacggccaag tggggtggtg
     1441 ggtgcgcggt ccgctatgcc cccgatggca ccgaggaccg ccggatccac tttcccgcca
     1501 ggaaagtctc gtgcgtgacc tttggcggcg agggctatga cgagatgtac gtcactaccg
     1561 cgggcggcga tcagaaggaa acggatggcg ccggcgcggg cgcgctcttc cgggtgcgcc
     1621 tgggtattcg tggcgtgccg gagttcctct cgcgagtctg cctgtgagtg cggagaatgg
     1681 cttatcgcgg cagttcgggg cctttctgga ccatttgttt ggtgttgagg caggaacgcg
     1741 agcggcgatg ccgaagaggg taagagtggg gggtgaggag agtacatggg gactgggctg
     1801 agggtggagc gagactactc gctgagagag tgcaacacgt tcggggttga ggcgaaggcc
     1861 gcctactacg cgcgcgtgac aagccgcgaa gagctcgccg cgctgctgca agacaatccg
     1921 gtgcgcgacc gcgagaagct ggtgctcggg ggcgggagca acatcctctt cacgcgcgat
     1981 ttcgacggcg tggtgatcca caacgccatc gggggaatcg cggttgtcg
//
LOCUS       DAOSCV010000851         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36751_length_2027_cov_3.537525, whole genome shotgun sequence.
ACCESSION   DAOSCV010000851 DAOSCV010000000
VERSION     DAOSCV010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36751_length_2027_cov_3.537525"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLY56_16995"
     CDS             complement(<1..534)
                     /locus_tag="PLY56_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23226.1"
                     /translation="MTTFLNCLQVQRIGRWVFMALAWCFAASGAYAEAKPVPPQPQLL
                     VLVSTGRNGVDQVAISYEGEVSEAKARAELRALLKATGWKASDVMVDCQALSPGAARM
                     TSVSFAAPVVVPYPEGTLPVAEFVQAYKAYRHLVLVFQLSRPFRLTGPDSYEDQHVSI
                     RLQQGTGIHRYDVRVTDP"
     gene            850..>2027
                     /locus_tag="PLY56_17000"
     CDS             850..>2027
                     /locus_tag="PLY56_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23227.1"
                     /translation="MALVAALVALLLPGCRRETPAPAKALVYEHPNYARLSPAELLIE
                     GQRKGVMGRKQSLRDVRRVVETIAPLVAQSVEQKAILADLQALAGRRPVAELREELRR
                     WQEADLLLESGGDPEAVSAAGAVGVAQWLADTARRAGLRVDEREYARIVARARRLRAA
                     LSELRQQPAEWQRAEQPGGPPLGRDQWIARYESELAALERKRLEADERRDPRKAIFAQ
                     TRYLVRLWRRFGSLDWALQAYHGGERGAERTLGYFLSEGGKSPVSFADLYFGTSPRKT
                     PKTFSYFYGRADDHRYYAWRVQMAQEALALYRESPEKFRARWETLAPGYPMDRAWYPN
                     ELDAAFADVAALQAAYRSGRLLALPDAGESVVVETLAPLDPENERWYRGLRPEALGML
                     "
BASE COUNT          329 a          637 c          732 g          329 t
ORIGIN      
        1 gggatccgtc acgcgcacgt catagcgatg gatacccgtt ccttgctgca gacggatgct
       61 gacgtgctgg tcttcatagg agtccgggcc cgtgaggcgg aagggccggc tcagctggaa
      121 cacgagtacc agatgccgat acgccttgta agcctggaca aactcggcga ccggaagcgt
      181 gccctctgga tagggcacga cgaccggggc ggcgaaggag acgcttgtca tcctggcggc
      241 gccaggggag agcgcctggc agtccaccat cacatcgctc gctttccagc ccgtggcttt
      301 caggagcgcg cgcagttcgg cacgggcctt cgcctcgctc acctcgccct cgtagctgat
      361 ggcaacctga tccacgccgt ttcgcccggt ggaaaccaac acgagcagct gcggctgcgg
      421 tggcaccggt tttgcctcgg cgtacgcccc agaagcggcg aagcaccatg ccaacgccat
      481 gaacacccag cgtccgatcc tctgcacctg caagcaattc aggaacgtgg tcactccctc
      541 tcttgagagc aatactaaca tacacgggga gggcaagtca atgatcggac cagggcgctt
      601 cagtgcgatg ggggcagcca ggcacgctcg cgccgtgtcg ttgacggtag gaggggggat
      661 ggtacaatgc ttgtggcgga agcgcaaggc gtgatgctcc gggggcaagg tggcccgcac
      721 gcgagtggac gggagcccgc gcaggcagag ggggaggggt tgatgcacct tcacgcatct
      781 caccgcaaaa ggatggttcc ctggcgatgg gaggggcaag ctggccacgt ggggcggagt
      841 atcgcccgag tggccttggt tgccgcgctg gtcgccctgc tactccccgg gtgccggcgt
      901 gagacgcccg ctcccgcgaa ggcgctcgtc tacgagcatc cgaactatgc gcgcctctcg
      961 ccggcggagc tcctgattga gggccagcgc aagggagtga tggggcggaa gcaatcgctg
     1021 cgcgacgtgc gccgcgtcgt cgagacgatt gcgcctctgg tggcgcagag cgtcgagcaa
     1081 aaagcgatcc tggcggacct tcaggcgctg gcgggaaggc ggcctgtcgc ggagctgcgc
     1141 gaggagttgc gccgctggca ggaagccgac ctgcttttgg agtccggggg tgacccagaa
     1201 gcggtctctg ccgcgggcgc agtgggtgtg gctcaatggc tggcggatac cgcccgccgg
     1261 gccggcctgc gcgttgacga gcgcgagtat gcgcgcatcg tcgcacgggc gcggcggctg
     1321 cgcgccgcgc tgtcggagtt gcgccagcaa ccggcggagt ggcagcgcgc ggagcagccg
     1381 ggcggcccgc cactcggccg cgaccagtgg atcgcaagat acgagtccga gctggcggcg
     1441 ttggagcgca agcgcctgga ggcggatgag cgtcgcgatc cgcgcaaggc gatcttcgcg
     1501 cagacgcgct acctggtgcg gctgtggcgg cgctttggct cgctggattg ggcgcttcag
     1561 gcctaccacg gcggcgagcg cggcgcggag cgcactctcg gctacttcct ctccgagggg
     1621 ggaaagtcac ccgtctcctt tgccgacctt tactttggca cgtcgccacg gaagacgccc
     1681 aagaccttct cctatttcta cgggcgtgcc gatgaccacc gctactacgc ctggcgtgtg
     1741 cagatggcgc aggaggcact cgcgctctac cgtgagagcc cggagaagtt ccgcgcgcgt
     1801 tgggagacgc tcgcccctgg ctacccgatg gatcgcgcgt ggtatcccaa cgagctggac
     1861 gctgcctttg ccgacgtggc cgctttgcag gcggcttacc ggagcgggcg cctgctcgct
     1921 ctgccggatg ccggagagag cgtggtggtg gagacgctgg ccccgctcga tccggagaat
     1981 gagcgatggt accgcgggct tcgccccgag gctctcggga tgctcca
//
LOCUS       DAOSCV010000852         2023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36844_length_2023_cov_4.227642, whole genome shotgun sequence.
ACCESSION   DAOSCV010000852 DAOSCV010000000
VERSION     DAOSCV010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36844_length_2023_cov_4.227642"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PLY56_17005"
     CDS             <1..778
                     /locus_tag="PLY56_17005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ23228.1"
                     /translation="RYFERHNGAQVGATRSLGQGRTLSLSGRFEDVQVFNLPPEFLAP
                     GIPSLDSSLLAGSGEYIIDRRNSLLYPTIGTYTDFAAELGWVSRGPDEGESGTLLKPS
                     AEWRVFIPIDRERALALSETTRQRTRVLALRAMAGTSLGELPFFEQYFVGGANTLRGY
                     REDRFWGEHMFVANAELRWPIGTGLIGVAFLDVGHAWGSDFQFTDAPGFSTAFRQSRD
                     LSLQLGAGLGVRYLSPLGPLRLDFGYGDELRVHFTLGQTF"
     gene            complement(899..1498)
                     /locus_tag="PLY56_17010"
     CDS             complement(899..1498)
                     /locus_tag="PLY56_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOJ23229.1"
                     /translation="MADTSIRVLTSARTARVHPVARALALTTLFALLTATGAQIALRV
                     PFTPVPVTLQVFFVLLSGALLGSRLGALSQLQYLGLGLTGLPVFAGGTTAATVLAGPT
                     AGYLLGFVVAAWIVGRVVESAPRPGAIRILAGMSIGMVAYYLPGTLWLALWLHTVSGV
                     PGVQALTTAAAVGTLPFIAVDIAKAVVATGIARGARIRR"
     gene            complement(1591..>2023)
                     /locus_tag="PLY56_17015"
     CDS             complement(1591..>2023)
                     /locus_tag="PLY56_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23230.1"
                     /translation="RSVLRESLALLASPAGEFLLKGKRLPDLPMRLPKLSLSLVPADA
                     KAPEERDYPRVLSASYELPGGRLGHLLIAMGDERERLDIPIKASGLPGQRLRLNLYQL
                     ENGKVTLQSETVMTPCVYPVNLSPFSPVFLESIPAGWEILW"
BASE COUNT          338 a          655 c          711 g          319 t
ORIGIN      
        1 acgctatttc gagcggcaca acggcgcgca ggtgggcgct acccgcagtc tggggcaggg
       61 gaggacgcta tcgctttccg gccggttcga ggatgtccag gtgttcaacc tgcccccgga
      121 gttcttggct cccggaatac ccagcctgga tagctcgctc ctggccggca gcggcgagta
      181 catcatcgac cggcgcaact cgctcctcta tcccaccatc ggcacctaca ccgactttgc
      241 cgcggagctg ggctgggtga gccgtggccc ggacgagggt gagtcgggta cgttgctcaa
      301 gcctagcgcc gaatggcgtg tcttcatccc catcgaccgc gagcgcgctt tggccctctc
      361 ggaaaccacg cgccagcgca cgcgggtgct ggcgctacgg gcgatggccg gcacatcgct
      421 tggcgagctt cccttcttcg agcagtactt cgtgggcgga gccaacacct tgcgcggcta
      481 ccgcgaagac cgcttctggg gggagcacat gttcgtggca aacgcggagc tgcgctggcc
      541 cattggcacc ggtctgatcg gcgttgcctt cctcgatgtg gggcacgcct ggggcagcga
      601 cttccagttc accgatgcgc ctggcttcag caccgccttc cgccagagcc gagacctctc
      661 cctccagctg ggcgcggggc taggcgtgcg ctacctgagc ccgctggggc ccttgcgcct
      721 ggattttggc tatggcgacg agctccgggt ccacttcaca ctggggcaga cgttctagcc
      781 agccgcgggc acttccacgg taggatgggg tggggttttg tccctggtgc ccgcccgtga
      841 tgggtagggg agggatgcat ccaaccctcc ttcccgatca ggggcgggca ttccggtctt
      901 accttcgaat gcgggcgcca cgcgcgatgc cggtggcgac gaccgccttg gcgatgtcga
      961 ccgcgatgaa ggggagcgtg ccgacagccg cggcggtagt gagcgcctga acccccggaa
     1021 cgccgctgac cgtgtgcagc cacagcgcca gccagagcgt gccaggcaga tagtaggcca
     1081 ccattccgat ggacatgccg gccagaatcc gaatggcgcc cggccggggc gcgctctcca
     1141 caacccgacc gacgatccag gcggctacca caaagccgag aaggtagcct gcggtgggcc
     1201 cggccagcac ggtcgcggcg gtggtgccgc ccgcgaagac agggagcccg gtgaggccca
     1261 gaccgaggta ctggagctga ctcaacgcgc ccaggcggct gcctagaagg gcccctgaaa
     1321 gcagcacgaa gaagacctgg agggtgaccg gcaccggagt gaagggcaca cgcagggcga
     1381 tctgcgcgcc ggtcgcggtc agcagggcga agagggtggt gagtgccagc gcgcgtgcca
     1441 caggatgcac tcgggcggta cgggcgctgg taaggactcg gatcgatgta tcagccaacg
     1501 gtgtctcctc ccggcccacc tctgcgtggg tgtcggcgct tattctacac gaagccggcg
     1561 ggcgcgtcaa ggccagcggc gggggtcctg ctaccacagg atttcccaac cggcagggat
     1621 gctctccaga aaaacagggg agaagggcga caggttgacc gggtagacgc agggcgtcat
     1681 gacggtctcg ctctggagcg tgaccttgcc gttctccaac tgatagaggt tgagtcgcag
     1741 gcgctggccc gggagccccg acgccttgat cgggatatcg aggcgctcgc gttcgtctcc
     1801 catcgctatc aggagatggc caagacgccc gccgggcagc tcgtagctgg cgctgagcac
     1861 gcgcggatag tcgcgctcct ctggcgcctt cgcgtcggcg gggaccaggg agagggagag
     1921 cttcggcagg cgcatcggca ggtccgggag ccgcttcccc ttcaacagga attcgcccgc
     1981 cggagaggcg agcagggcga gcgactcgcg caacaccgag cgc
//
LOCUS       DAOSCV010000853         2020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36921_length_2020_cov_3.606107, whole genome shotgun sequence.
ACCESSION   DAOSCV010000853 DAOSCV010000000
VERSION     DAOSCV010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36921_length_2020_cov_3.606107"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PLY56_17020"
     CDS             complement(<1..164)
                     /locus_tag="PLY56_17020"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876964.1"
                     /note="regulates pyrimidine biosynthesis by binding to the
                     mRNA of the pyr genes, also has been shown to have uracil
                     phosphoribosyltransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HOJ23231.1"
                     /translation="MDEQDIRRALTRIAHEIVERNQGAVDIVLVGLYTRGVAIGQRLA
                     AAIAGFEGVAV"
     gene            complement(454..1020)
                     /locus_tag="PLY56_17025"
     CDS             complement(454..1020)
                     /locus_tag="PLY56_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23232.1"
                     /translation="MPKPLLPEPLLRTLQRQGITVTAYLDAPRKRSHGRFERREVWAM
                     HWPELNRYLGSAGEAGEVWPQVEQICWVKRERLTAKAHSVEISYAVTSLRPEEADARR
                     LCKEWRRYWGAVENSSHCVRDLTLQEDASQVRTGAGPQVMAALRNLMLALARRAGASN
                     IAAALRAYAGRFEQAVTIVLTAGMPRDR"
     gene            complement(1112..1780)
                     /locus_tag="PLY56_17030"
     CDS             complement(1112..1780)
                     /locus_tag="PLY56_17030"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HOJ23233.1"
                     /translation="MGGDAEWPPGSLAAALATIPDPRRRQRRTHSLVAILQVSVAAML
                     CGARSIYAIGQWARERLEDAPELLEALGVRKGKCPSVPTLHRVFKRMPAADFEEKIGR
                     WLQATGLEPGETIGIDGKTLRGIHGEEVPAVHLVSAYAVHARAVIGEIACQGKGHELE
                     AVRQLIERLPLQGNLVVGDALQCQRDLCARIVERGGDYLVPVKENQPALRADIQRAFS
                     PAGP"
BASE COUNT          335 a          712 c          593 g          380 t
ORIGIN      
        1 accgccactc cctcgaagcc ggcgatcgcg gcggcgaggc gctggccgat ggcgacgccc
       61 cgggtgtaca gcccgacgag cacgatgtcg accgcgccct ggttgcgctc gacgatctcg
      121 tgggcgatgc gggttagggc ccggcggatg tcttgctcgt ccatcacccg cgtactctgt
      181 agtgcagcca cagtcactcc cgcgggcgcc gccggggaac cggcccgcgc ccggaaccta
      241 acgaaaaggg agcctccaaa cgccttcgcg agcgtatgga ggctccctaa aatgagcgca
      301 agccatatcc ccttaccggc ctcacgggac cggcttaaag gcgattggat gacccagccg
      361 cgtctgcgcg gcccaagcta aaatcgagtc agtttatcac gcccctggcc ccggcgtcaa
      421 gaggcgatct gacgtttgtc agggcctttt cattcagcgg tcacggggca ttccagcggt
      481 caagacgatg gtgaccgcct gctcaaaccg acccgcatag gctcgtaggg cagccgcgat
      541 gttgctcgcc cctgcacgcc gcgctagggc aagcatcagg ttccgcaggg ccgccatcac
      601 ctgcggcccg gccccggtcc gcacttggct ggcgtcttcc tgcaaggtca ggtcccgcac
      661 gcagtgggag ctgttctcca ccgcacccca ataccgccgc cactccttgc agagacggcg
      721 ggcatccgcc tcctccggcc gcagactcgt cacggcgtag cttatctcta ctgagtgtgc
      781 ctttgccgtc agccgttctc gcttcaccca gcagatctgc tccacctgtg gccacacctc
      841 gcccgcttct cccgcactgc ccaggtagcg gttcagctcc ggccagtgca ttgcccacac
      901 ctcgcgccgc tcgaaccgtc cgtgagagcg cttgcggggc gcatccagat aagcggtcac
      961 ggtgatcccc tgccgctgca gcgttcgtaa cagtggctca ggcagtaacg gcttcggcaa
     1021 ttcctcggcg ggaagggccc ggacgggggt ttggccttcc gtaagcaacg cgcgcgctgc
     1081 ctcgacgcgt gcgggcagct tggcagaggg atcaaggccc agcaggggaa aaagcccgct
     1141 ggatatcggc tcgcagtgcc ggctggttct ctttcaccgg caccaggtag tcgcctcccc
     1201 gttccacaat gcgcgcgcag aggtcacgct ggcactgcag ggcatcgccg actaccaggt
     1261 tcccctgaag tggcaacctc tcgatcagtt gtcttaccgc ttcgagttcg tgccccttcc
     1321 cttggcaggc gatctcccca atcaccgccc tggcatgcac ggcgtaggcc gacaccaaat
     1381 gcaccgccgg cacctcttct ccatggatcc cacggagcgt cttcccgtca atgccgatgg
     1441 tttccccggg ttccagaccg gtggcctgca gccaccggcc gatcttctcc tcgaagtcgg
     1501 cggcaggcat ccgcttgaag acgcggtgca aggtgggaac gctcgggcat ttgcccttgc
     1561 gtacgccaag cgcttccagt aactccgggg catcttccag gcgttctcgc gcccactggc
     1621 cgatagcata gatgctcctg gccccgcaaa gcatcgcggc cacggacacc tgcagaatgg
     1681 caaccagcga atgcgtccgc cgctgcctac gtcgcgggtc aggaatagtg gccagggctg
     1741 ccgccaggga accaggaggc cactctgcat ctccgcccat tctcgtcctc ctctgaatag
     1801 caccggcttg cgaggctcct acctattcta tacccccctc accctaaatg aaaaggccct
     1861 gttgcgggca gaatctgccc ttgacacgtt cttgttgatg tgttatccta tggcaggtgg
     1921 ccctttttcg ctccacttag ctccgccccg tctgtgttga ttcccaagag agctcgcagt
     1981 tgcgtgaccg ggtccggtgt gtggcggctt tgggcaccgg
//
LOCUS       DAOSCV010000854         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_36986_length_2018_cov_2.571065, whole genome shotgun sequence.
ACCESSION   DAOSCV010000854 DAOSCV010000000
VERSION     DAOSCV010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36986_length_2018_cov_2.571065"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLY56_17035"
     CDS             <1..474
                     /locus_tag="PLY56_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23234.1"
                     /translation="DQGETFALSQFGVTMRLEAGADRRIGGGPGHTFLNAKWLVLPEE
                     RGLPAVAVGIQNVAGGLRAEPFLVGTRSLAQTRFHLGTVRTEGALRPLAGIDHTVGTL
                     TLLAEVLGGPEGIAAGGIAVPLTSRLGLTYAATLPTRGGQVGHIVNLALSVGKTL"
     gene            661..1380
                     /locus_tag="PLY56_17040"
     CDS             661..1380
                     /locus_tag="PLY56_17040"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOJ23235.1"
                     /translation="MLQRIWRVILSVITLGLVKLETPELVGDQLIEEMEAKERKLAED
                     AIGVVAYQKRLEAELAQAEKDLATWTNRAKAAARAGDQEVGIHAMEQVTRLEANIQRL
                     QESVAVARDRSRQAMEALERFKAQVASARERVKDLKARDRLARLESNASRAMTGYDLN
                     AQMKQLDRMSEQVAEREARARATTEIASNDINVRIQRAEMDAQRAEAANRFAELQAEV
                     AGTGEAQAAPPVPEAEGGRQL"
     gene            complement(1474..>2018)
                     /locus_tag="PLY56_17045"
     CDS             complement(1474..>2018)
                     /locus_tag="PLY56_17045"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ23236.1"
                     /translation="YCNTVEGDRFEDYLMQDLMAHIEGSYNVLKGREHTAIGGLSMGG
                     YGAMKLGLKFPERFGSVGAHSSAFAVARKAWDEPTEREMRRIFGPADSPARAENDPWV
                     LAERLAKEPERLPTLYIDCGTEDFLLECNREFHAHLNRLGIAHTYQEFPGTHCWEYWD
                     QHIQDSLAHHCKALGIEPAA"
BASE COUNT          342 a          651 c          712 g          313 t
ORIGIN      
        1 gaccaggggg agacgttcgc gctctcgcag tttggggtga cgatgcgcct ggaggccggc
       61 gcggaccggc ggatcggagg aggccccggg cacacattcc tcaatgccaa gtggctggtg
      121 ttgccggagg agcggggttt gccggccgtg gccgtgggca tccagaatgt ggcgggtggt
      181 ctgcgcgccg agcccttcct ggtgggcacg cggagcctgg cccagacacg ctttcatctc
      241 gggacggtgc gcaccgaggg ggccttgcgc ccactggcag gcatcgacca caccgtcgga
      301 acactgacgc tgttggcgga ggtgctcggc ggaccggagg ggatcgccgc cggcggcatc
      361 gccgtgcctc tcacttcccg acttgggctc acctacgcgg cgacactgcc cacgcgcggc
      421 ggccaggtcg ggcatatcgt gaatctggcg ctgtcggtcg gtaaaaccct gtaggcgcgc
      481 cgtgccagcg cctctccgaa tgccacaggc gttggctcca cggggcgaaa tagcctcgtg
      541 gagcgacggc taagctggcg acgctgaggg cacgtgccct tgacacaacg ccccaaacgg
      601 cgtattattc tccctgacta agcgcccgcg tcacgggcat gcccagaagg gggacgaacc
      661 atgctgcaga ggatctggcg ggtcattctc tccgtcatca cgctgggact cgtcaaactg
      721 gagacgccgg aactggtggg cgaccagttg attgaggaga tggaggccaa ggagcgcaag
      781 ctcgccgagg atgccatcgg tgtcgtggcc tatcagaaac gcctcgaggc ggagctggcc
      841 caggcggaga aggatctggc gacatggacc aaccgggcga aggccgccgc gcgcgcgggc
      901 gaccaggagg tcggcatcca tgccatggag caggtgacgc ggctggaggc gaatatccag
      961 cggctccagg agagcgttgc cgtcgcgcgt gaccgatcca ggcaggcgat ggaggcgctg
     1021 gagaggttca aggcgcaggt cgcgtccgca cgcgagcgcg tgaaggatct caaggcgcgg
     1081 gatcgcctcg ctcgtctgga gtccaatgcc agccgcgcca tgaccggcta cgacctcaat
     1141 gcacagatga agcagctcga ccgcatgtct gagcaggtcg ccgagcgaga ggcgcgagca
     1201 cgtgccacca ccgagatcgc gtccaacgac atcaacgtcc ggatccagcg ggccgagatg
     1261 gacgcccagc gcgctgaggc cgccaaccgc ttcgcggagc tccaggccga ggtcgccgga
     1321 acgggcgagg cccaggcggc gcctcccgtg ccggaggcgg agggtggacg ccagctctag
     1381 gcgcgctcca attcacatgg atgccgggtc tcttgccccc cgcgcgcggg gggcaagccg
     1441 gagtatcctt tcatcgcgat cggggcgggc ggactacgcc gcgggctcga tcccgagcgc
     1501 cttgcagtgg tgcgccagcg agtcctggat atgctggtcc cagtactccc agcaatgggt
     1561 tccggggaac tcctggtagg tgtgcgcgat cccgaggcga ttcaggtggg cgtgaaactc
     1621 gcggttgcac tcgagcagga agtcctcggt gccgcagtcg atgtagagcg tgggcagccg
     1681 ctccggctcc ttcgcgaggc gctccgcgag cacccacggg tcgttctcgg cccgggccgg
     1741 ggaatcggcc ggaccgaaga tgcggcgcat ctcccgctcc gttggttcgt cccacgcttt
     1801 ccgcgcgacg gcgaaggcgc tcgagtgcgc gcccacggat ccaaaccgct cggggaactt
     1861 caggcccagt ttcatcgcgc catagccgcc catcgatagc ccgccaatcg ccgtgtgctc
     1921 gcgccccttg agcacgttgt acgatccctc aatgtgggcc atcaggtctt gcatcaggta
     1981 atcctcgaag cgatcccctt ccaccgtgtt gcagtaga
//
LOCUS       DAOSCV010000855         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37001_length_2017_cov_3.355250, whole genome shotgun sequence.
ACCESSION   DAOSCV010000855 DAOSCV010000000
VERSION     DAOSCV010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37001_length_2017_cov_3.355250"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PLY56_17050"
     CDS             complement(<1..700)
                     /locus_tag="PLY56_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ23237.1"
                     /translation="MGAPVVEITALRKRFVVRHERLPSVKRLVSRLLRPFPTHTLWAL
                     QDLDLSIHAGEAVGLVGHNGSGKSTLLRLIAGIFRPTSGSIVVRGRVGGLFELAAGFS
                     PELSGRDNIYLSAALMGYSAAAVRKRYDSIVEFSELGDFIDVPVRTYSSGMSLRLGFS
                     IAVAFEPEVLLVDEVLAVADERFQRKVYRTLNQARERGAAVILVSHELRAIQEVCPRV
                     VWLDHGQAQADGPAE"
     gene            complement(700..1515)
                     /locus_tag="PLY56_17055"
     CDS             complement(700..1515)
                     /locus_tag="PLY56_17055"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ23238.1"
                     /translation="MGYGRGAASPLRWSWAVSQLVRRDLKVRYKNSVLGFCWSFLNPL
                     MQIVVMTIVFKYIMGSRINNFSMELFTCFLPWLFFAQSLNDGAVCVAKDNLLVRKYAF
                     PRIILPIASITSNLVHLFFGFLVLFAIFLVLPVHFNRHFLWVIPLLAIQFIFTLGLVL
                     LVGTLQMYYEDIRFLLNSVVQLLFFLCPTVYTVEQVMESTRLSPLWKELYILLNPLTP
                     LFIGYRTALLHGAEWPVPGYYSYLAVSALWALLFLGVGLLVWRRYEWHFAEVC"
     gene            complement(1658..>2017)
                     /locus_tag="PLY56_17060"
     CDS             complement(1658..>2017)
                     /locus_tag="PLY56_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23239.1"
                     /translation="RPEALGMLQRLVAIYREEGGRAPLSVCGATATVEYAAREQTRGD
                     AASDGEEVPALTLHAVGLCVDVERPADSWDRKVLEYALSTLGDRMRIAWLRDYSANAY
                     HLCPNPAFAREFAPRRR"
BASE COUNT          445 a          671 c          622 g          279 t
ORIGIN      
        1 cctccgccgg gccatcagcc tgcgcctgac cgtggtcgag ccagaccacg cgcgggcaga
       61 cttcctgaat ggcgcgcaac tcgtgggaga caagaataac ggcggcgccg cgctcgcgcg
      121 cctggttcag cgtgcgatac accttccgtt ggaagcgctc gtcggccacg gcgagcactt
      181 catccaccag gagcacctcc ggctcgaagg caacggcgat ggagaagcca aggcgcagcg
      241 acatgcccga ggagtaggtg cgcaccggca catcgatgaa gtcgccgagc tccgaaaact
      301 cgacgatgga gtcgtaccgc ttgcgcaccg ccgccgccga atagcccatc agggcggcgc
      361 tcagatagat gttgtcgcgc ccgctcagtt ccggactgaa gccggccgcc agctcgaaga
      421 ggccgcccac acgcccgcgc acgacgatgc tcccgctcgt cggccggaag atgccggcga
      481 tcagccgcag cagcgtgctc ttgcccgagc cgttgtgccc gacaagcccg accgcctcgc
      541 ctgcgtgtat cgacaggtcg agatcctgga gtgcccagag cgtatgcgtg ggaaacgggc
      601 gcagcaggcg cgagaccagc cgcttcacgc tcggtaaccg ctcgtgccgg acgacgaagc
      661 gcttccgaag agcagtgatc tccactaccg gggcgcccat cagcacacct ccgcgaagtg
      721 ccactcatag cgtcgccaca ccagcagccc cacgcccaga aagaggagcg cccagagcgc
      781 cgaaaccgcc aggtaggagt agtagcccgg caccggccac tccgcaccgt gcagcagcgc
      841 cgtgcgatag ccgatgaaga gcggcgtcag cgggttcagc aggatgtaaa gctccttcca
      901 aagcggcgag agacgcgtgc tctccatcac ctgctcgacc gtgtagaccg tcgggcagag
      961 gaagaagagg agctgcacca ccgagttgag gagaaagcgg atatcctcat agtacatctg
     1021 aagcgtcccg accaggagca ccaggccgag cgtaaagatg aactggatcg ccagcagcgg
     1081 gatcacccac aggaagtggc ggttgaagtg aaccggcaac accagaaaga tggcaaagag
     1141 caccagaaac ccgaagaaga ggtgaaccag gttcgatgtg atcgaggcga tcggcaagat
     1201 gatccgcggg aacgcatact tgcgcacgag caggttatcc ttggccacgc atacggcgcc
     1261 gtcattcagc gactgcgcga agaagagcca gggcaggaag caggtgaaaa gctccatcga
     1321 gaagttgttg atccgcgacc ccataatgta cttgaagaca atcgtcatca cgacgatctg
     1381 catcagcggg ttgaggaagg accaacaaaa gcccaacacc gagttcttat agcgcacctt
     1441 cagatcgcga cgcactagct gggagacagc ccaactccag cgcagcggcg aggcggcccc
     1501 tctgccatag cccatcacgg ttttccagat gacccggaca acgattgctc ctctcgcgcg
     1561 gccctacccg gaaggcaggg cggagcgccc gcccactctt ccctatggat gcgactatca
     1621 taccgaatgc agacccgaac gtcaagcagg caggcactca tcggcgccgg ggcgcaaact
     1681 cacgcgcgaa ggccggattg gggcaaaggt gataggcatt ggccgaatag tcgcgcagcc
     1741 aggcgatccg catccggtcg cccagcgtgc tcagggcata ctccagcact tttctgtccc
     1801 aggaatctgc cgggcgctcc acatccacac acaggccgac ggcgtgcagc gtgagcgctg
     1861 gcacctcttc tccatccgaa gcggcatccc cgcgcgtttg ctcgcgcgcg gcatactcca
     1921 ccgtcgcggt cgcgccgcac accgagaggg gcgcgcgccc gccctcctcg cggtagatag
     1981 ccaccaggcg ttggagcatc cccagggcct cggggcg
//
LOCUS       DAOSCV010000856         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37105_length_2013_cov_2.518897, whole genome shotgun sequence.
ACCESSION   DAOSCV010000856 DAOSCV010000000
VERSION     DAOSCV010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37105_length_2013_cov_2.518897"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="PLY56_17065"
     CDS             <1..1488
                     /locus_tag="PLY56_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23240.1"
                     /translation="DLQRRAYRHEEAWDWAGLAGGLYANQDLYYVLIMALSAELYQRP
                     EDLEEAKAMLRKVAESQLPDGGFRYIGQENEAPVYHALNLIILGRYAKLTGDPLALKL
                     LKQTANYWPLVLTAEAQPEYWSDVWWKQSWGYVWREALVIAAGATGDERVQWLMWRAL
                     ERAAPDDGGVGGIYCAPWWTGTAPGQAMPERFVARDRNMRGIRGRAGQWYFGVTQGRG
                     LRNTFTGGLVTSPVAPQPLLAAFRGAQIHVLEDETRYTTWFLSEREDRTALALRPDVA
                     GALGARYTLQPGKINSVPTPETPPSPWQVTQVWRAAGDGILGMVVLEATQDARAAAVA
                     GRIGLGRRQVREVAPGMWACGPLRVKLLRQFGTVSAQPVPGYNMPREKGWPGVEMLQR
                     IPGGAKRGDRFVYAIWVGNESSTPPDTLDLLQGDTGWVARWADGRSVAALFNPDAQPR
                     VARVPWTGPAPTAWIGETPETRKLDLADGMASIQLPPGACALVEQ"
     gene            1495..>2013
                     /locus_tag="PLY56_17070"
     CDS             1495..>2013
                     /locus_tag="PLY56_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23241.1"
                     /translation="MTAGMAKLFDWEDVGMRHGAGRETRSGRRPYRWLSAALGIAMAL
                     LARAGTAQAAGFPPVVRGARAVLLRPDPGPLTIRITKRDLNIYDGPDTLAAKLYDPRR
                     RLVGTLELPDDGNTGKGLATETQSGELKLTCETPGVYRLVIACNHDLVFGLETSSKGV
                     MIEGDILLNDGNV"
BASE COUNT          324 a          665 c          743 g          281 t
ORIGIN      
        1 gacctgcaac gccgggccta ccggcacgaa gaggcgtggg attgggccgg gctggccggc
       61 ggcctctacg cgaatcagga tctctactac gtgctgatca tggccctctc cgccgagctt
      121 taccagcgcc ccgaagacct cgaagaggcg aaggcgatgc tccgcaaagt ggcggagagc
      181 cagctgccgg acggcggttt ccggtatatc gggcaggaga acgaggcgcc cgtctaccac
      241 gcgctcaact tgatcatcct ggggcgctac gcgaagctca ccggcgatcc cctggccctg
      301 aagctgctga agcagaccgc caactactgg ccactggtcc tcaccgccga ggcccagccg
      361 gagtactgga gcgacgtgtg gtggaagcag agctggggct acgtgtggcg cgaggcattg
      421 gtgatcgcgg ccggcgcgac gggcgatgag cgcgtccagt ggctgatgtg gcgcgccctg
      481 gagcgggcag cgcccgatga tgggggcgtc ggaggcatct attgcgcgcc gtggtggacc
      541 ggaaccgcgc ccggccaggc gatgccggag cgcttcgtgg ctcgcgaccg caacatgcgc
      601 ggcatccgcg ggcgcgcggg ccaatggtat ttcggcgtga cccaaggccg cggcctgcgc
      661 aacaccttca ccggaggcct agtcaccagt ccggttgcac cgcagccgct gctggcggcg
      721 ttccgcggcg cgcagatcca cgtgctggag gatgagacac gctacaccac ctggttcctc
      781 tcggagcgcg aggatcgcac ggcgctcgcg ttgcgcccgg atgtggccgg cgcgctggga
      841 gcgcgctaca cgctccagcc cgggaagatc aacagcgtgc ccacgccgga aacgcccccg
      901 tcgccctggc aggtgaccca ggtgtggcga gccgcgggcg acggcatcct cggaatggtg
      961 gtcctggagg cgacccagga tgcgcgcgcg gctgccgttg ccgggcggat cgggctgggc
     1021 cgacgccagg tgagggaggt cgcgcccggg atgtgggcgt gcgggccgct ccgcgtgaag
     1081 ctcctgcgcc agtttggcac cgtctcggcg cagccggtgc ccggctacaa catgccgcgc
     1141 gagaaaggct ggcccggtgt ggagatgctc caacggatcc ccggcggcgc caagcgcggc
     1201 gaccggtttg tttacgccat ctgggtgggg aacgagagct cgaccccgcc ggacacactc
     1261 gacctgctgc agggcgacac gggctgggtg gcacgctggg cggacgggcg cagcgtcgct
     1321 gccctcttca acccggacgc gcagcctcgg gtggcgcggg tgccctggac cgggccggca
     1381 cccaccgcct ggatcggcga gacgccggaa acccggaagc tcgatctggc agacggtatg
     1441 gcgtccatac agctcccgcc cggcgcgtgc gcgctcgtgg agcagtgaga gcgagtgacg
     1501 gcaggcatgg cgaaactgtt cgattgggag gacgtgggaa tgcgtcacgg agcaggtaga
     1561 gagacgcgaa gcgggaggcg cccttatcgc tggctttccg ccgcgctggg gatcgcgatg
     1621 gcgctgctgg cgcgagcggg gacggcccag gcagccggtt ttccgcccgt ggtccgaggc
     1681 gcgcgcgcgg tgctcctgcg cccggacccg ggcccgctca cgatccggat cacgaagcgc
     1741 gacctgaaca tctacgacgg cccggacacg ctcgcggcca agctgtacga cccgcgccgc
     1801 cgcctcgtcg gcaccctgga gctcccggac gacgggaaca ccggcaaggg cctggcaacg
     1861 gagacgcaat cgggcgaact gaagctcacc tgcgagacgc caggtgtgta ccggctcgtc
     1921 atcgcgtgca accatgacct ggtgttcggt ctggagacgt ccagcaaggg cgtgatgatc
     1981 gagggcgata tcctcctgaa cgatggcaac gtc
//
LOCUS       DAOSCV010000857         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37222_length_2009_cov_2.576766, whole genome shotgun sequence.
ACCESSION   DAOSCV010000857 DAOSCV010000000
VERSION     DAOSCV010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37222_length_2009_cov_2.576766"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..895)
                     /gene="mreC"
                     /locus_tag="PLY56_17075"
     CDS             complement(71..895)
                     /gene="mreC"
                     /locus_tag="PLY56_17075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ23242.1"
                     /translation="MFPRVDRNLVRLLLALLIVVLLAAGHRRATSAGEESLGATIVAV
                     TLSPFQRTLVSSARMLENAWGIVREIRTLYAENQRLRARVEQLEQRVNILNERALENQ
                     RLREMLRFRETLPRSGENALAATVIGVKPTNAFNTITVDRGANDGVQAQQMVLTPRGL
                     VGQVRVVTPNTATVLLITDLTSGVGGRVQRTGWRGVVRGTAGPLLEMTYIPKEADVRR
                     GDLVVTSGVGSIFQVKGIVIGKVENVRLDENISVKTATVRPAVDFRRLEEVLVLTQ"
     gene            complement(900..1943)
                     /locus_tag="PLY56_17080"
     CDS             complement(900..1943)
                     /locus_tag="PLY56_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ23243.1"
                     /translation="MSFWGSVYSRFSRDMGIDLGTANTLVHVRGRGVLLREPSVVAIN
                     SEDGSPLAVGVEAKRMLGRTPGSIIAVRPLKDGVIADFEQTEAMLRYFTHKVHRRKWF
                     LGPQVIIGVPSGVTEVERRAVRSAAQRAGARWVGLIEEPMAAAIGAGLPVAEPVGSMI
                     VDIGGGTTEVAVISLGGVVTHRSLRVAGDEMDEAIISYVRRVHNLLIGDRTAEEVKIN
                     IGSAYPMEEERTMEIRGRDLVSGLPRSAVLTSSEVREAIAEPVSQVVETVKETLESTP
                     PELSADIMDRGIILAGGGALLRGLDRLLAVETEMPVHTAEDPLTCVVMGAGRVVEEMD
                     RNPILKKALISKD"
BASE COUNT          356 a          694 c          607 g          352 t
ORIGIN      
        1 agcgccaatg cgatgatcat cgcgccgatg acgatcggca gccgcagtcg aggggtgggg
       61 cggtccagac tcattgcgtc aacacgagca cctcctcgag acggcgaaag tccaccgcgg
      121 ggcggaccgt cgcggtcttc acggagatgt tctcatcaag acggacgttt tctaccttac
      181 cgatcacaat gcccttcact tgaaagatcg atccaacccc cgaggtgacc accaggtcgc
      241 cccgacgcac gtctgcctcc ttcgggatgt aggtcatctc cagaaggggg ccagctgttc
      301 cccgcaccac gccgcgccag ccggtacgct gcacgcgccc gcccaccccg ctggtgaggt
      361 cggtgatcag gagcaccgtt gccgtattcg gggtgaccac gcgcacttgg cctaccagcc
      421 cccggggcgt gagcaccatc tgctgcgcct gcacgccgtc gtttgcccca cgatccacgg
      481 tgatcgtgtt gaacgcgttc gtgggcttga ctccgatgac cgtggccgcc agagcgttct
      541 cgcctgagcg gggcaaggtc tcgcggaagc gaagcatctc gcgcaggcgc tggttctcca
      601 gcgcgcgctc attgaggatg ttcacgcgct gctcgagctg ctccaccctg gcccgcaggc
      661 gttgattctc ggcgtagagg gtgcggatct cgcgcacaat cccccaggcg ttctccagca
      721 tccgggcgct gctcaccagg gttcgctgga agggcgacaa cgtcaccgcg acgattgtcg
      781 cgcctaggct ctcctctccc gccgaggtgg cccggcggtg acccgcggca agcaggacga
      841 cgatgagcag agcgagcaac aggcgcacca ggttgcggtc gacccgaggg aacatcgtct
      901 taatctttgc tgatcagggc ctttttgagt atcgggtttc ggtccatctc ctccaccacg
      961 cgtccggcgc ccatgactac gcaggtgagc gggtcctcgg cggtatgcac gggcatctcc
     1021 gtttccacgg cgagaagccg gtcgagcccg cgcagcagcg cgcccccgcc ggccaggatg
     1081 atcccgcggt ccatgatatc cgcgctcaac tcgggcgggg tgctctcgag cgtctccttg
     1141 acggtttcca ccacctgcga gacgggctcg gcaatcgctt cgcgcacctc ggacgaggtg
     1201 agcaccgcgc tgcgtgggag accgctgacc aggtcgcgcc cacggatctc catggtgcgc
     1261 tcctcttcca tcgggtaggc cgatccgatg ttgatcttca cctcttcagc cgtgcggtca
     1321 ccgatcagga ggttatgtac tcggcgcacg tatgagatga tggcctcatc catctcatcg
     1381 ccggcgacgc ggagcgagcg atgggtgacc acgccgccca gcgagatcac cgcgacctcg
     1441 gtggtgcctc cgccgatatc gacgatcatg ctgccaaccg gctcggcgac gggaagcccg
     1501 gcgccaatcg cggcggccat cggctcctca atgaggccca cccaccgcgc gccagcgcgt
     1561 tgcgcagccg agcgcaccgc gcgccgctcc acctccgtca cgccggaggg cacgccgatg
     1621 atcacctgtg gccccaggaa ccacttgcgc cggtgcacct tgtgcgtgaa atagcgcagc
     1681 atcgcctccg tctgctcaaa gtcggcgatg acgccatctt tcagtgggcg aaccgcgata
     1741 atagaaccag gggttcggcc cagcattcgc ttggcttcga cgcctacggc aagtggcgag
     1801 ccatcctcac tgttgatcgc cactacggaa ggctcacgaa gcaaaacccc acgcccacgc
     1861 acatgcacca gcgtgttcgc ggttcccaga tcaatcccca tgtcgcggga aaaacggctg
     1921 tagaccgacc cccagaatga catagcgttc gatataactc ctgtagtatt gtgagtagat
     1981 cagacaccaa acgcgccggt ctccatgcg
//
LOCUS       DAOSCV010000858         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37282_length_2006_cov_3.607893, whole genome shotgun sequence.
ACCESSION   DAOSCV010000858 DAOSCV010000000
VERSION     DAOSCV010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37282_length_2006_cov_3.607893"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLY56_17085"
     CDS             complement(<1..489)
                     /locus_tag="PLY56_17085"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23244.1"
                     /translation="MEKLNVAVVGLGMGRHHVRCFKNCPEANVVALCDADPTRLNNAG
                     DEFGVKQRFTQTEELFAMKDLHAVSIALPNFLHAPVTIAALKAGLHVMCEKPLAMNAQ
                     EAEQMVAAAKAADRQLMVHFNTRYQEPSRWARQIVDAGVVGPIYFGRTGWHRSRGIPG
                     GLG"
     gene            complement(596..>1311)
                     /locus_tag="PLY56_17090"
     CDS             complement(596..>1311)
                     /locus_tag="PLY56_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ23245.1"
                     /translation="MAAEDRLVRDPEGTQHNWANPGDPRNQELEYQVALDLIKRYDVD
                     GYHLDYIRYPEVPHTRFDYGEVSRREFERASGVRVKRWPQDVRTGSMRERYEEWQREN
                     ISRLVKRIYTGIKEVKPKVQVSAAVWRNYPSCRDGVRQEWPRWVRAGWLDFVVPMNYV
                     LDAGEQEQMVRAHIREVGGARPVIAGIGSWQLHSPGEVLAHVRAARAGGAKGFVLFSF
                     NDEAIGDHLQVLSAGACSP"
     assembly_gap    1312..1321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1322..>2006)
                     /locus_tag="PLY56_17095"
     CDS             complement(<1322..>2006)
                     /locus_tag="PLY56_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23246.1"
                     /translation="SRGETTLPAATLNKAGMLVGHLLTADDPHRKRAFLLAALAHLDS
                     SLWGQAASTRRAAAASRLEAQRARWKRLSSHPGLAGRGPAVERYVAEARAALAGVAQR
                     PPSDAHHGRAAYQKVEAAERALDCLESALTPAPAGELRGFWIHTYQPTDWDSVMAKAK
                     AGGLNAAFVRVGRGGNTLYPNPLLPLDPWAKEAGGDELQRAIDAARKHGLAFHAWRVV
                     YHLGSAPREY"
BASE COUNT          306 a          694 c          654 g          342 t
ORIGIN      
        1 gcccaggcca ccgggaatgc cgcggctgcg gtgccatcca gtgcgcccga agtaaatcgg
       61 gccgaccacg ccggcgtcca cgatctggcg cgcccaacgg ctcggctcct gataccgcgt
      121 attgaagtgg accatcagct gccggtctgc ggctttggcc gccgcgacca tctgctcggc
      181 ctcctgcgcg ttcatcgcga gcggcttctc gcacatgacg tgcaggccgg ccttcagcgc
      241 cgcgatggtg accggggcgt ggaggaagtt ggggagcgcg atggagacgg catggaggtc
      301 cttcatcgcg aagagctcct ccgtctgggt gaagcgctgc ttgaccccaa actcatcacc
      361 ggcgttgttc aggcgcgtcg gatcggcatc gcaaagcgcg accacgtttg cctcggggca
      421 attcttgaag cagcggacgt ggtgccggcc catcccaagg ccgaccacgg ccacattcag
      481 cttttccatg ggagcaggtt ccttccgtat cgctggcgcg tggatgcgcg cgccaggctt
      541 tgccgggctc tgattcgacg cccgcgcctc gaatgccttg cacaggcgcg ggaagctatg
      601 gcgaacacgc acccgcggag agcacctgga gatggtcgcc aatcgcctcg tcgttgaagc
      661 tgaagaggac gaagcccttc gccccgccgg cgcgcgcggc acgcacgtga gccagcacct
      721 ctcccggcga gtggagctgc cagcttccaa tccccgcgat cacggggcgc gcaccgccca
      781 cctcgcgaat gtgagcgcgc accatctgct cctgctcgcc cgcgtcgagg acgtagttca
      841 tcggaacgac gaagtcgagc catccggcgc gcacccaccg tggccactct tggcgaaccc
      901 catcccggca ggatgggtag ttgcgccaga cggcggcgga gacctgcacc ttcggcttca
      961 cctccttgat cccggtgtag atccgcttga ccaggcgcga gatgttctct cgctgccact
     1021 cctcgtagcg ctcgcgcatg cttccggtgc gcacatcctg tggccagcgc ttcacgcgca
     1081 ccccggacgc gcgttcgaac tcgcggcgcg acacctcgcc ataatcaaac cgcgtgtggg
     1141 gcacctccgg gtagcggatg taatcgaggt gatatccatc cacgtcatag cgtttgatca
     1201 ggtcgagcgc cacctgatat tccagctcct ggttgcgcgg gtcgcctgga ttcgcccagt
     1261 tgtgttgcgt tccctctgga tcgcgcacta ggcgatcctc ggcggccatg cnnnnnnnnn
     1321 nggtattccc gtggagcaga gccaaggtga tacaccacgc gccaggcatg gaaggcgaga
     1381 ccgtgcttgc gcgcggcatc gatcgcccgc tgcagttcat cgccgccggc ctccttcgcc
     1441 caggggtcga gcggcaggag cgggttgggg tagagggtgt tcccgccgcg gccaacgcgg
     1501 acaaacgctg cgtttagccc accggctttc gccttcgcca tgacggagtc ccagtccgtc
     1561 ggctgatagg tgtggatcca gaagcctcgc agctctcctg ccggcgccgg ggtgagggcg
     1621 ctctccaggc aatccagcgc gcgctcggcg gcctcaactt tctgataggc ggcccgaccg
     1681 tggtgcgcgt cggatggcgg cctctgcgcc acgccggcca gcgcagcacg cgcctccgcc
     1741 acgtagcgtt cgaccgctgg cccgcgtccg gccagacctg ggtgactgct caggcgcttc
     1801 cagcgcgcgc gttgcgcctc caggcggctc gccgcagccg cccggcgcgt gctcgccgcc
     1861 tggccccaga gggatgaatc gaggtgggcg agggccgcga ggagaaaggc tcgcttccga
     1921 tgcgggtcat ccgccgtcag caggtggccc acaagcatcc ctgccttgtt cagggttgct
     1981 gcgggaagcg tcgtctcgcc acgaga
//
LOCUS       DAOSCV010000859         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37389_length_2002_cov_3.481253, whole genome shotgun sequence.
ACCESSION   DAOSCV010000859 DAOSCV010000000
VERSION     DAOSCV010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37389_length_2002_cov_3.481253"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..886)
                     /locus_tag="PLY56_17100"
     CDS             complement(11..886)
                     /locus_tag="PLY56_17100"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ23247.1"
                     /translation="MIIDIHTHTIMLNDPRQRAISRWTSPEELLSMWDELGIEKGVVL
                     PLVHHEIGTILQTTENVLDIYAKWPDRIIPFCNLDVRWCYNRPDSDFTPVLEHYKELG
                     CKGVGEFVNNLPWDDDRVMNFLSHVEKAGLPLLFHVASRERGQYGLIDDYRLTKLEKV
                     LEAFPKLQFIGHSMGFWSNISGDANEENWMGYPKGPVTPGGRLIELMRTYDNLWGDIS
                     AGSGNNALVRDKEFGFRFLEEFQDRLLIVTDICSPGQQVPQVETMREALASGLLSQTA
                     YEKICYKNAEKLLGL"
     gene            complement(950..1834)
                     /locus_tag="PLY56_17105"
     CDS             complement(950..1834)
                     /locus_tag="PLY56_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ23248.1"
                     /translation="MQSLLERVKAGEILISDGAMGTFLQEKGLKPGECPEAWCISHPD
                     VVRGIAAAYVEAGSDIVETNSFGGTRFKLKEYGLEARVAEFNRAAARLAKEAMGDRGY
                     VAASVGPTGQIVEDEGGEATEEQLYEAFREQMIALAEGGADAFCIETLSSVIEASVAV
                     RAAKENTRLPVICTFTFELGQRGYRTMMGADPARAARAAVEAGADIVGANCGNGIAQM
                     IEIVAQIRAALPDVPILVHANAGMPVLENGKSVFKETPADMAARVPELIAAGAQIIGG
                     CCGTTPAHIAAIAKAARG"
BASE COUNT          338 a          687 c          603 g          374 t
ORIGIN      
        1 gccccccctc ttacaggccc agcagcttct cggcgttctt gtagcagatc ttctcgtaag
       61 ccgtctggct cagcagtccg ctcgctagtg cttcccgcat cgtctccacc tggggcacct
      121 gctggccggg cgagcagata tcggtgacga tgaggaggcg gtcctggaac tcctcaagga
      181 agcggaagcc aaactcctta tcgcgcacga gggcgttgtt gccgctcccc gcggagatgt
      241 cgccccacag gttgtcgtag gtgcgcatca actcgatcag gcggccgccc ggggtgacgg
      301 ggcccttggg atagcccatc cagttttcct cgttcgcgtc gccgctgatg ttggaccaga
      361 aacccatcga gtggccgatg aactgcagtt tcgggaaggc ttccagcacc ttttccagct
      421 tggtcagacg atagtcatcg atcaacccgt actgccctcg ctcgcggctc gccacgtgga
      481 agaggagcgg caggcccgcc ttctccacgt gcgagaggaa gttcatcacg cgatcatcat
      541 cccacgggag gttgttcacg aactcgccca cgcccttgca cccgagctcc ttgtagtgct
      601 ccaaaacggg cgtgaagtcg gaatccggcc ggttatagca ccagcgcacg tcgaggttgc
      661 agaaggggat gatccggtcg ggccatttgg cataaatgtc caggacgttc tcggtcgtct
      721 ggaggatcgt gccgatctcg tggtgcacta ggggcaggac gacgcccttc tcaatcccca
      781 gctcatccca catcgagagg agctcctccg ggctggtcca acgactgatc gctcgctgcc
      841 gcgggtcgtt cagcataatc gtgtgcgtgt gaatatcgat gatcattcca acctcatctt
      901 tccgctcggc tcgcgtgcca ccgcagggtg actcaggcgg tctggcgcgc tagccacgcg
      961 ccgccttcgc tatcgccgcg atatgcgcgg gcgtcgtccc gcaacacccg ccgatgattt
     1021 gggcgcccgc ggcaatcagc tcgggaacgc gcgcggccat atcggccggc gtctccttga
     1081 agacgctctt gccgttctcc agcaccggca tcccggcgtt ggcatggacc agaatcggca
     1141 catccgggag ggcggcgcgg atctgcgcca cgatctcgat catctgagcg atgccgttgc
     1201 cgcagttggc ccccacgatg tcggcgccgg cctcaacggc cgcccgcgcg gcgcgcgccg
     1261 gatcggcgcc catcatcgtt cggtagccgc gctggcccaa ctcgaaggtg aaggtgcaga
     1321 tgactggcag gcgcgtgttc tccttcgctg ccctcaccgc cacggatgcc tcgatgacag
     1381 aggagagtgt ctcgatgcag aacgcatcgg cgccgccctc agccagcgcg atcatctgct
     1441 cgcggaacgc ctcgtacagc tgctcctccg tggcctcgcc accctcgtcc tcgacgatct
     1501 gccccgtcgg ccccacggag gccgcgacgt agccccggtc gcccatcgcc tccttggcca
     1561 ggcgcgcggc agcccggttg aactcagcaa cccgcgcctc caggccatac tccttcagct
     1621 tgaagcgagt gccgccgaac gagttggtct ccacgatatc ggagcccgcc tcgacataag
     1681 cggcggcgat accgcgcacc acgtcgggat gactgatgca ccaggcctcg gggcactcgc
     1741 ccggcttcag gcccttctcc tgcaggaagg tacccatcgc gccatccgag atgaggatct
     1801 cgccggcttt cactcgctcc agaaggctct gcatgcttct actcctttgc ctgttgccac
     1861 taaacgccca acttgcagcg agcaacctct tccgggataa ggcgcggagc aggctcccga
     1921 acgcacaacc ggagagcggg atcgaccaat cgttggggcg tgggaagatg ggagtgggcg
     1981 ttgcgcgcgg ctctctcctg aa
//
LOCUS       DAOSCV010000860         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37611_length_1993_cov_3.975748, whole genome shotgun sequence.
ACCESSION   DAOSCV010000860 DAOSCV010000000
VERSION     DAOSCV010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37611_length_1993_cov_3.975748"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1993)
                     /gene="nifJ"
                     /locus_tag="PLY56_17110"
     CDS             complement(<1..>1993)
                     /gene="nifJ"
                     /locus_tag="PLY56_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ23249.1"
                     /translation="ATTKAIAVLDRTKEPGAAGEPLYQDVLTALAEGMANGSMPCNSF
                     PKVIGGRYGLSSKEFTPAMVKAVLDELAKADPKNHFTIGITDDVTNTSIEVDADFHTE
                     SDDVVRAVFWGLGSDGTVGANKNSIKIIGEETPNYAQGYFVYDSKKSGSVTVSHLRFG
                     PRPIHSTYLVQSANFVGIHQFGFVERYPVLKNAAEGATVLLNSPFGPEETWDNLPRPI
                     QEEILNKKLKLYVIDAYEVARQTKMGVRINTIMQTCFFALSGVLPREEAIEQIKKAIK
                     KTYGKRGEAVVQQNFAAVDAALANLYEAKVPGQVTSTRGLAAAVPAGAPEFVQNVTAD
                     IIANQGDQIPVSKLPVDGTFPTGTAAWEKRNIALDVPVWDPELCIQCGKCVLSCPHAA
                     VRSKIVPQNLLAEAPEGFKSAPAKWRELADQRFTIQISVEDCTGCTLCMESCPAKDKT
                     QVGRKALTMHPQRPLREEGARNWEFFLQLPDMPRDTDKVRFNNVKNVQLLQPLFEFSG
                     ACAGCGETPYVKLVSQLFGDRMVVANATGCSSIYGGNLPTTPWTKNRDGRGPAWANSL
                     FEDNAEFGLGMRLSIDTQTRYARQLVSQLRERIGIALADALLAADQSDEAGIQAQRAR
                     VAELKSRLAGATDSAGRDLLAVADFLVKKSVWILGGDGWA"
BASE COUNT          314 a          619 c          689 g          371 t
ORIGIN      
        1 ggcccagccg tcgccaccca ggatccagac gctcttcttg acgaggaagt ctgccacggc
       61 gagaaggtcg cggccagcgg aatcggtggc gccggcgagg cgcgacttca gctcggccac
      121 gcgcgcgcgc tgcgcctgga tcccggcttc gtcactctga tcagccgcga ggagggcatc
      181 cgccagggcg atgccgatgc gctcgcgcag ctgagagacg agctggcggg cgtagcgggt
      241 ctgggtatcg atggagaggc gcatccccag gccgaactcg gcgttgtcct cgaagagcga
      301 gttggcccac gccggcccgc gcccgtcgcg gttcttcgtc cacggggtgg tcggcaggtt
      361 gccgccgtag atgctggagc atcccgtggc gttggcaacg accatgcggt caccgaagag
      421 ctggctgacg agcttgacgt agggcgtctc gccgcagccg gcgcatgcac cggagaactc
      481 gaagagcggc tgcaggagct gcacgttctt cacgttgttg aagcgcacct tgtcggtatc
      541 gcgcggcata tccggaagct ggaggaagaa ctcccagttc cgagcaccct cttcgcggag
      601 cggccgctgg ggatgcatgg tgagcgcctt gcgcccaacc tgcgtcttat ccttcgccgg
      661 gcagctctcc atacagaggg tgcagccggt gcagtcttcc accgagatct ggatggtgaa
      721 gcgctggtcg gccagttcgc gccacttcgc cggcgcggac ttgaagccct cgggtgcctc
      781 ggcgagcagg ttctggggca cgatcttgga gcggaccgcg gcatgcgggc agctcaggac
      841 gcacttgccg cactggatgc acagctccgg atcccagacg ggaacatcca gggcgatgtt
      901 gcgcttctcc caggcggccg ttcccgtggg gaaggtgcca tcgaccggga gcttgctgac
      961 ggggatctga tcgccctggt tggcgatgat atccgcggtc acattctgca cgaactccgg
     1021 cgcgccggcg ggaaccgcag cggccaggcc gcgggtgctg gtcacctggc cgggcacctt
     1081 ggcctcatag aggttggcca gcgccgcgtc aaccgcagcg aagttctgct ggacgaccgc
     1141 ctcgccgcgc ttgccgtagg tcttcttgat cgccttcttg atctgctcga tcgcctcttc
     1201 gcgcgggagc acgccgctga gcgcgaagaa gcacgtctgc atgatcgtgt tgatgcgcac
     1261 gcccatcttc gtctggcgcg ccacttcata cgcgtcgatg acgtacagct tcagcttctt
     1321 gttgaggatc tcttcctgga tcgggcgagg aaggttatcc cacgtctcct ccgggccgaa
     1381 ggggctgttg aggagcaccg tggcgccctc ggctgcgttc ttcagcacgg ggtagcgctc
     1441 gacgaacccg aactggtgaa tccccacgaa gttcgcgctc tgcaccaggt aggtgctgtg
     1501 gatcgggcgg ggaccaaagc gcagatgcga aacggtgacc gacccggact tcttggagtc
     1561 gtacacaaag taaccctgcg cgtagttggg cgtctcctcg ccgatgatct tgatcgagtt
     1621 cttgttggcg ccgacggtgc cgtcggagcc gaggccccag aagacggcgc ggacgacgtc
     1681 atccgactcg gtgtggaagt cggcatccac ctcgatgctg gtgttggtta cgtcatcggt
     1741 gatcccgatg gtgaagtggt tcttgggatc ggccttggca agctcgtcga ggacggcctt
     1801 gaccatggcc ggcgtgaact ccttggagga gaggccgtag cggccgccga tgaccttcgg
     1861 gaaggagttg cagggcatcg agccgttggc catgccttcg gcgagggccg tgagcacgtc
     1921 ctgatagaga ggctcgcctg cggcgccagg ctccttcgtg cggtcgagga ccgcgatcgc
     1981 cttcgttgtg gcc
//
LOCUS       DAOSCV010000861         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37630_length_1993_cov_2.459236, whole genome shotgun sequence.
ACCESSION   DAOSCV010000861 DAOSCV010000000
VERSION     DAOSCV010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37630_length_1993_cov_2.459236"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..826)
                     /locus_tag="PLY56_17115"
     CDS             complement(53..826)
                     /locus_tag="PLY56_17115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ23250.1"
                     /translation="MPTEDSYRAVAYPAPEAMARIRIILVEPQHPGNIGASMRAMKTV
                     GMSDLVLVRPVPFRESQDTWNLASGARDLVESARVVDDLDAAAEGVHWLVGTTNRKRD
                     SLLDAPVPVREAARRLASIATERRVGILFGREDYGLSSADLARCNQSVAIPVAEGMPP
                     LNLSHAVQVVAHELFMAVLGAPPPAPVKQATVSEVEALLGRFSELFALLGPDALSRPS
                     AQVLASLRRIFSRVGLEPRDVRTLHMIVRAVTRRLRGSG"
     gene            1103..1471
                     /locus_tag="PLY56_17120"
     CDS             1103..1471
                     /locus_tag="PLY56_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ23251.1"
                     /translation="MRGYRGKRKAQRRWRDAACAVLWIGALALVVLGGVLGLGRAMQR
                     SRAETPALREVVLPAGGTLWGLAIRYGAPSQDPRAVAAQIRRLNGIRPGQTLHPGTRL
                     LVPDYAGGATELAQNSHGTG"
BASE COUNT          347 a          606 c          705 g          335 t
ORIGIN      
        1 gtttttgcct cgccctcccc gcggccgcgg agaagtagcg gacggtcctg aactaccccg
       61 agccgcgcag gcggcgtgtc accgcgcgca cgatcatgtg gagggtgcgc acgtcgcgcg
      121 gctccaggcc gacgcggctg aaaatccggc ggagcgaggc gagcacctgc gccgatgggc
      181 gcgagagcgc gtccggcccc agcagggcga acagctccga gaagcggccc agcagcgcct
      241 ccacctcaga gacagtggct tgtttcactg gtgctggcgg cggcgcgccg aggactgcca
      301 tgaacagctc gtgcgccacc acctgaacgg cgtgcgatag gttcaaagga ggcattccct
      361 cggcgacggg gatggcgacg ctttggttgc atcgcgccag gtctgccgag gagaggccgt
      421 aatcctccct cccgaacagg atcccgacgc gacgctcggt ggcgatgctg gcgagacgcc
      481 gggcggcctc gcgcacgggt accggcgcat cgagaaggct atcgcgcttc cggttggtgg
      541 tgcccacgag ccagtggacc ccctccgccg ctgcgtccag gtcatccacc acgcgcgcgg
      601 attccaccag gtcgcgcgct ccgctggcca ggttccacgt atcctgggat tcacggaacg
      661 gcaccggccg gacgaggacc aggtcgctca tcccgacggt cttcatggcg cgcatcgacg
      721 cgccgatgtt tccggggtgc tgtggctcta cgaggatgat ccggatgcgc gccatcgctt
      781 ccggcgcggg atacgcgacg gcgcggtacg agtcctcagt cggcatggtc tgcttatctc
      841 tcctgggggt gtgttatccc tggtgttctg tgccaagcag gcgccatcct cctggcgccg
      901 cgggagcgct attggtctca atcggggcag cgccggtagg ccgccggcca cccacgaacg
      961 gaccggggat aggagagcac cccgccattc ccgcccgttc ccttccactg caatatcacc
     1021 gctggtgtgc aaacgcttga caagcacaca ggtgtgtgct agaatgcaaa tccaaacgct
     1081 gtttgcgttc gggagggctg cgatgcgagg ctatcgaggc aagcggaaag cgcagcggcg
     1141 gtggcgggat gccgcgtgcg cagtgctctg gatcggggcg ctggccctgg tggtgctggg
     1201 aggtgtgctg ggcctgggcc gtgccatgca gcggtcgcgg gcggagaccc cggcgttgcg
     1261 cgaggtggtg ttgccggccg gcggcacctt atggggactg gcgatacgct acggcgcgcc
     1321 gagccaggat ccgcgcgccg tcgccgcgca gatccggcgc ctgaacggga tccgcccggg
     1381 acagacgctc catccaggaa cgcggctact tgttccggac tatgcgggtg gggcaaccga
     1441 attggcacag aactcgcacg gaacaggcta gcagcgacgg gcctgagcag cgagtttggt
     1501 gcctaggtgt tgtgggtata cttcgggcgt actggaaagt ttgctcgatg taagagcaac
     1561 caaggctccc agagaagggc acgggaggcg agtgccaggg gtacctgcaa gaggcaaaag
     1621 cccggatggt gcatacacag gagggacctg gaccttgaaa cggtgatacc gcgcgccggc
     1681 cgccgagagc ggtgcgacgc tgtaaccgac ttctatggaa aagaaagggc aggggaaccc
     1741 cttcgactct atctctagta gagaccaggg tggtgccctg ccctttgtca tctcttgtgc
     1801 aggcaggttc cggtgtcggt tgccggttgc atgagtacca cgagaaaggg agggaggatg
     1861 atggatttac tcgctcggct tgaggagtac cgggcccagg agaatgagct ccgatgggag
     1921 ggaacgttcg aagagtattt cgagctggtg aaggccaacc cgcggatctc ccggctttcc
     1981 catgctcgcg tat
//
LOCUS       DAOSCV010000862         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37758_length_1988_cov_3.302638, whole genome shotgun sequence.
ACCESSION   DAOSCV010000862 DAOSCV010000000
VERSION     DAOSCV010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37758_length_1988_cov_3.302638"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLY56_17125"
     CDS             <1..787
                     /locus_tag="PLY56_17125"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ23252.1"
                     /translation="EAAQETISRLLASAHAGRIYREGARVVIAGRPNVGKSSLLNALL
                     REDRAIVTEIPGTTRDVIEESANIRGIPIRAIDTAGIREPGDPIEAIGVQRTLEQVRA
                     ADLILVVLDASQTLHEADQEVFHLVHGEPCVIVANKCDLPVRLDLNGIRAEFPAAPIV
                     QVSAVQGTGLDQLDEQIAETLLGSGVELGNQALVTSARHQAALDRARMALQRACETLD
                     QDLPLELATTDLRDALDALGEITGDTVSEAILAEIFGRFCIGK"
     gene            complement(989..>1988)
                     /locus_tag="PLY56_17130"
     CDS             complement(989..>1988)
                     /locus_tag="PLY56_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23253.1"
                     /translation="GSGWYGAASTTTESPMMAQGDPWSNDLVPVRALARLALIDGDPA
                     PTTVATTTTRGPDRLVTGVAQWMNPSDNSLYEIRFTGIDTSGPLDGAGVLRVIHGETG
                     IGSPQFPRTLAYAALFGPAQIFRNGQMIASDLQGQMMVTQGIRTPDTGRLLDTPNVRP
                     GDLQFHVILQGQIPGRNQDTLYLFWPSATVDIRNLGTPVALLPQEIQTARSYFTEGAV
                     AGYREPTVPTTPTTPTAAPTASRILVSMRDRFLARSTPELAAGDVELSITNDSTVARG
                     FFIQGPGVEQRTRLLNPGESTTMTVRLSPGTYRLASFTVATPSADDLMHMDTITVR"
BASE COUNT          331 a          637 c          693 g          327 t
ORIGIN      
        1 cgaggccgcc caagagacga tctcgcgcct gctggcgagc gcgcatgccg ggcgcatcta
       61 ccgtgagggc gcgcgcgtgg tcatcgcggg ccggccgaac gtcgggaaat ccagcctact
      121 caacgcgctc ctccgcgagg atcgcgccat cgtcaccgag attccgggca cgacgcgcga
      181 cgtgattgag gagagcgcca atatccgggg catccccatc cgcgccatcg atacggccgg
      241 tattcgcgag ccgggagacc ccatcgaggc gattggggtg cagcgaaccc tggagcaggt
      301 gcgcgcggcc gatctgatcc tggtggttct ggacgcgtcc cagacgcttc acgaggcgga
      361 ccaggaggtc ttccacctcg tgcacggcga gccgtgcgtg atcgtcgcta acaaatgtga
      421 tctaccggtg cgactggacc tgaatggcat tcgcgcggag tttcccgccg cgcctatcgt
      481 ccaggtttca gcagtgcagg gcaccggtct ggatcagctg gacgagcaga tcgcggagac
      541 gctgctggga tccggggttg agctggggaa ccaggcgctg gtgacgagcg cgcgccatca
      601 ggccgccctc gatcgggcgc ggatggcgct tcagcgcgcc tgtgaaacgc tcgaccagga
      661 tctgcccctc gaactcgcga caacggatct tcgcgatgcc ctcgacgcgc tgggcgaaat
      721 caccggagac accgtctccg aagcgatcct cgccgagatc ttcggtcgct tctgcatcgg
      781 caagtagagc ccggcgccca ggagcatccc cggaagcgag ttgagcccgt ggaagcccaa
      841 gccccgcctg tgaccgcgca ggcggggctt cgcagtgact caatacccgt cccggcaggc
      901 atggcgccga ccagggccgt gcctgccagc cgctgcgtca actggaagca cgtgcttccg
      961 gtggtcaagt gcctccccga tgcctatgct accgcacggt gatggtgtcc atatgcatga
     1021 gatcatccgc gctcggagtg gccacggtga aggatgccag gcgataggtg cctgggctga
     1081 gacggaccgt catcgtggtc gactcgcccg ggttgaggag acgggtgcgc tgctcaacgc
     1141 cggggccctg gatgaagaag ccccgtgcga cagtggagtc gttcgtgatg ctcaattcca
     1201 catcgcccgc ggcgagttcc ggtgtgcttc gcgccaggaa gcgatcgcgc atgctgacca
     1261 ggatccgcga ggccgtcggc gctgctgtcg gcgtcgtcgg cgtggtcggc accgtcggct
     1321 cgcgatagcc tgcaactgct ccctcagtga agtaactgcg cgcggtctgg atctcctggg
     1381 gcaggagcgc caccggggtt cccaggttgc ggatatcgac cgtggccgag ggccagaaga
     1441 gatagagcgt gtcctgattg cggccgggga tctgcccctg caggatcacg tggaactgga
     1501 gatcgcccgg gcggacattg ggcgtgtcaa ggagccggcc cgtatccggc gtgcggatcc
     1561 cctgagtcac catcatctgc ccctgcaggt cggacgcgat catctggccg tttcggaaga
     1621 tctgggccgg gccaaacagc gcggcgtagg cgagggtgcg cgggaactgg ggtgacccga
     1681 tccctgtctc gccatgaatg acgcggagca cgccggcgcc gtcgagcggt ccagacgtgt
     1741 cgatcccggt gaaccggatc tcatacaggg agttgtccga cgggttcatc cattgggcca
     1801 ccccggtaac gaggcggtcg ggcccccggg tggtagtcgt ggccaccgtg gtcggcgcag
     1861 gatccccatc gatcaatgcc aggcgcgcga gggcccggac gggtaccagg tcgttcgacc
     1921 agggatcgcc ctgtgccatc atggggctct cggtagtggt cgatgcggcg ccataccagc
     1981 ccgagccc
//
LOCUS       DAOSCV010000863         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37786_length_1987_cov_3.184783, whole genome shotgun sequence.
ACCESSION   DAOSCV010000863 DAOSCV010000000
VERSION     DAOSCV010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37786_length_1987_cov_3.184783"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="PLY56_17135"
     CDS             complement(<1..1532)
                     /locus_tag="PLY56_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23254.1"
                     /translation="MRWVAGGALLLLLMASTAMAESRPPLPCTPVPGYPVPPLAESAP
                     PEKVTWLRQVPIHAARVRGVPRSEPLDGCDSSTLPLVEPLEVRLVRSALVSYLELRGA
                     GTLALGRAGCVSPGVQLEPVARLLLSVPEKERRFSIEPVDGTMLAEVRLYEAAEEPLP
                     ETALAMALASQEKGAAAITLSTGALAQPMGVTAVLLELAAATPIPFPLRVRLTHSAET
                     DRTWFEADIHPQADAGSVGGRWIVVLDPPDIWCEAGEHLVLSVTPLPEAGAGPPDPAA
                     SMAGVQARAALVPGTEQEVGIEFALSRLQWLDAWYAAHAVDAPWERPGWSLDDPAAAD
                     ARRWLEVLSRWQPRHPATIAYTSRLRRLRSGNELPAAGPANAPAWARLARQLLKRTQD
                     VAYWWNSTRYQKDGLYGGDPAGDAALVERLATVPLIAGHPVLLRSTRAGCEGLWRAMQ
                     ARATAGNAGADPEAALAAWVAELPAGERERWLLAITRTQSLLLLADPGNATPLRRLLP
                     FAR"
     gene            complement(1573..>1987)
                     /locus_tag="PLY56_17140"
     CDS             complement(1573..>1987)
                     /locus_tag="PLY56_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23255.1"
                     /translation="EGVGKLAPVDADASLIGTRIQDLLLSPDGKRLYVASDSLTYVSV
                     LDAETLRPLGQLEVGQPPAAVALSADGQTAYVCHRAPRIDRFDTRTFLRTGTLSLPAA
                     SLRCAVSPDGKKLFAQLATGIVIRGTDAFGPPTPE"
BASE COUNT          319 a          705 c          683 g          280 t
ORIGIN      
        1 cgcgcgaagg ggagaagccg gcgcaggggg gttgcatttc cgggatcggc gagcaggagc
       61 agcgattgcg tccgggtgat tgccaggagc cagcgctccc tttccccggc cggcagctca
      121 gccacccacg cggccagtgc ggcttccgga tccgcgcccg cgttgcccgc ggtagccctg
      181 gcctgcatcg cgcgccagag gccctcgcac cccgcccgtg tcgagcgcag gagtaccgga
      241 tgcccggcga tcagcggcac tgtggccagc ctctcaacga gggccgcgtc gcccgctggg
      301 tcgccgccgt agaggccatc cttctggtag cgcgtgctgt tccaccagta ggccacatcc
      361 tgagttcgct tcagaagctg gcgcgccagc cgtgcccatg ccggagcatt tgctggtccc
      421 gctgccggca actcgtttcc actgcgcagg cgccgcaacc ggctggtgta ggcgattgta
      481 gccggatgcc tcggctgcca acgcgacaac acctccagcc agcggcgtgc gtccgctgcc
      541 gcggggtcgt ccagcgacca tccgggccgc tcccacggcg cgtccaccgc atgcgctgca
      601 taccacgcat ccagccattg caaccgcgag agcgcgaact cgatgccgac ttcctgttcc
      661 gtgcccggca ccagcgccgc ccgggcttgc acgcccgcca tagacgccgc cggatccggt
      721 gggccggcgc ccgcctcagg gagaggtgtt acggagagca ccagatgctc gcccgcttcg
      781 caccagatat ccgggggatc cagcacgacg atccaccgcc cgcccacgga tcccgcgtcc
      841 gcctgcgggt ggatgtcggc ctcaaaccag gtacggtccg tctccgcgga atgggtgagg
      901 cgcacgcgga gtggaaatgg aatcggtgtg gctgccgcca gttccaggag caccgcggtc
      961 actcccatcg gctgcgcgag cgctcctgtc gagagtgtga tcgccgccgc gcccttctcc
     1021 tgcgaagcga gcgccatcgc cagcgcggtt tcgggcagcg gctcctccgc ggcttcatac
     1081 aaccgaacct cggcaagcat ggtgccatcc accggctcga tggagaagcg ccgttccttc
     1141 tccggcactg agaggaggag ccgggccacc ggctcgagct gcaccccagg ggagacgcag
     1201 cccgcgcgcc cgagcgccag ggtgccggcg ccgcgtagct ccagatagct cacgagcgcc
     1261 gagcgcacca gccggacctc cagcggttcc accaggggaa gcgtcgagct atcacagcca
     1321 tccagcggct cgctccgggg cacgccgcgc actcgcgccg cgtggatcgg aacctggcgc
     1381 agccacgtca ccttctccgg tggcgcgctc tccgcgagag ggggcacggg gtagccgggc
     1441 acgggcgtgc agggaagcgg cgggcgggat tcggccatcg ccgtcgatgc catcaggagc
     1501 aggagcaagg cgccccccgc tacccatcgc aacaaccctg caaatcgaga gcctccaggg
     1561 cgtggcatgc cgtcactcgg gagttggggg accaaaggcg tcggtgccgc ggatcacaat
     1621 gcctgtcgca agctgagcaa agagcttctt gccgtcgggg gagacggcac agcgcaggga
     1681 tgccgcggga agtgaaagcg tgcctgtgcg cagaaaagtg cgcgtgtcga accgatcgat
     1741 gcgaggcgcg cggtggcaga cataggcggt ttgcccgtct gcgctgagcg ccacggcggc
     1801 gggcggctgt cccacctcca gctgacccag cgggcgcagc gtctccgcgt ccaacaccga
     1861 gacgtaggtg agcgagtcac tcgccacgta gaggcgcttg ccatccgggc tgaggagcag
     1921 gtcctggatg cgggtgccaa tgagactggc atcggcgtcc acgggggcga gtttcccaac
     1981 gccctcg
//
LOCUS       DAOSCV010000864         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37831_length_1985_cov_3.913990, whole genome shotgun sequence.
ACCESSION   DAOSCV010000864 DAOSCV010000000
VERSION     DAOSCV010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37831_length_1985_cov_3.913990"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLY56_17145"
     CDS             complement(<1..589)
                     /locus_tag="PLY56_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOJ23256.1"
                     /translation="MRAVLLIAICLIFASPARGDGAANSAAFDEPRVTLSFSGADLLE
                     TLYRLFQGTGFSYEAPRDAPEMFSADLENVPLETALRVVLEPRGFTFTREGRLYRIAR
                     REGYRSVAEQLEEEDRALERRRIIESIQRPPRREPRPRVRFAHAPALAGFAPRPVTGM
                     LPGTYLNGSWVLPSLPANPRPTQPAPEQGGSQTLRL"
     gene            complement(666..1544)
                     /gene="dapA"
                     /locus_tag="PLY56_17150"
     CDS             complement(666..1544)
                     /gene="dapA"
                     /locus_tag="PLY56_17150"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOJ23257.1"
                     /translation="MELGRLLTAMVTPFDSDLNVDYKAAERLAQHLIDTGTQGIVVSG
                     TTGESPTLTTAEKLELFKVVKGVAAGKAAVIAGTGNYCTAESIELSRQAEKIGVDAVM
                     LVAPYYNKPSQEGLYQHFKAVAQSISIPVILYNVPTRTVTNIEPQTIIRLSEIPNIVS
                     VKEASKNLEAVAEIRANTPPSFLIYSGDDGVTLPIMAVGGHGIISVAGHLAGKLIREM
                     VDKFAAGDTAGALELHLKLLPLFKAIFVTTNPVPVKYGLNRIGVPVGGVRLPLVEANE
                     KERAVVDAALQGLGLI"
     gene            complement(1641..>1985)
                     /locus_tag="PLY56_17155"
     CDS             complement(1641..>1985)
                     /locus_tag="PLY56_17155"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ23258.1"
                     /translation="TPPPVATPRELRLPETPTVDGRSPEGTPTLDLGAQAKSPPPGLA
                     ALASHYAALGLPPTANLEQVGRAYRRLKERCNPEQFPDDPIQQARARQEEARLDEAYL
                     TLCKALARERRL"
BASE COUNT          339 a          638 c          654 g          354 t
ORIGIN      
        1 cgaggcgcag cgtctgcgag ccgccctgct ccggcgcagg ttgagtgggg cgcgggttcg
       61 cgggcaggga aggcaggacc caggagccgt tcagataggt accgggaagc atccccgtga
      121 cgggacgcgg ggcgaacccg gccaatgcgg gcgcatgtgc gaagcgcacg cgcgggcggg
      181 gctctcgacg cgggggacgt tggatcgact cgatgatgcg ccggcgttcc aacgcgcgat
      241 cctcctcctc cagctgctcg gcgacactcc ggtagccctc gcgcctggca atccggtaga
      301 gccggccctc gcgcgtgaag gtgaagccac gcggttccag caccacgcgc agtgccgtct
      361 ccaggggcac gttctccagg tcggcagaga acatctcggg cgcgtcccgg ggagcttcgt
      421 aggagaagcc ggttccctgg aaaaggcggt agagggtttc gaggaggtcc gctcctgaaa
      481 aggagagagt gacgcgcggt tcgtcgaagg cggcggagtt ggccgcgccg tctccgcgcg
      541 cgggagaggc aaagatcaag cagatcgcaa tcaggagtac cgcacgcatc gccgcacccc
      601 cgatctcaaa cggaagcagg ggcccgagcg cagcgcctgg gcattcgggc ccctcatctt
      661 tgtggctaga ttagtcccaa tccctgcagg gccgcgtcca ccacggctcg ctctttctcg
      721 ttggcttcga ccagcgggag gcgcacacca ccgacgggaa ccccgatgcg gttgaggcca
      781 tacttcaccg gcacggggtt ggtcgtcacg aagatcgcct tgaagagggg cagcagcttc
      841 aggtgaagct ccaaagcccc ggcggtgtcg ccagcggcga acttatccac catctcgcgg
      901 atcagcttgc ccgccaggtg gcctgccacg ctgatgatgc catgcccgcc aacggccatg
      961 atcgggagcg tcacgccatc gtccccgctg tagatcagga acgatggcgg tgtgttcgcc
     1021 cggatctccg ccacagcctc gaggttcttg ctcgcctcct tgaccgacac gatattcggt
     1081 atctccgaga ggcgaatgat cgtctgcggc tcgatgttgg tcaccgtccg cgtgggcaca
     1141 ttgtagagga tgaccggaat ggagatgctc tgcgcgaccg ctttgaaatg ctggtaaagg
     1201 ccctcctgag aaggcttgtt gtagtagggc gccacgagca tcaccgcgtc aacgccgatc
     1261 ttctcggcct ggcgagagag ctcgatgctc tcggcggtgc aatagttgcc ggtgccagca
     1321 atgacggcag ctttgcccgc ggccacacct ttgacgacct tgaaaagctc caacttctca
     1381 gccgttgtca gcgtcggcga ctcccccgtg gtcccactga ccacgattcc ctgcgttccc
     1441 gtgtcgatga ggtgttgtgc caggcgctcg gcggctttat agtccacgtt gagatctgag
     1501 tcaaagggcg tgaccatcgc cgtgagcaat ctgccgagtt ccatgcaagt atcccttcct
     1561 gtagcggggc ttgttccccc tctcgtagta tagcgaaaag ccggaagggt gtcaacgaaa
     1621 agccctgcgc cctctccgct ttagagccgg cgctcgcggg caagcgcctt gcagagtgtg
     1681 agatacgcct catcgagacg cgcttcctcc tggcgcgccc gcgcctgctg gatgggatcg
     1741 tccgggaact gctccggatt gcaccgctcc ttgagcctgc ggtaagcccg ccccacctgc
     1801 tccagattgg cggtcggcgg caacccgagc gccgcgtagt gcgaagccaa tgcggccagg
     1861 ccgggaggag ggctctttgc ctgcgctccc agatccagcg tgggggtgcc ctctggacta
     1921 cgcccgtcca cggtgggcgt ctctggcagt ctcagctccc gaggtgttgc caccggaggc
     1981 ggcgt
//
LOCUS       DAOSCV010000865         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37836_length_1985_cov_2.941969, whole genome shotgun sequence.
ACCESSION   DAOSCV010000865 DAOSCV010000000
VERSION     DAOSCV010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37836_length_1985_cov_2.941969"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLY56_17160"
     CDS             <1..342
                     /locus_tag="PLY56_17160"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ23259.1"
                     /translation="IGGPFRYAILLDMVGDADLRIPRERYSHEQARAIVDKVWQAAAE
                     LGYSQFSASIGPAIYDDHLPLLRKGIPAIDIIDFDYPYWHTLQDTPDKCSPTSLKIVG
                     DVVRRVVYAER"
     gene            348..>1985
                     /locus_tag="PLY56_17165"
     CDS             348..>1985
                     /locus_tag="PLY56_17165"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ23260.1"
                     /translation="MQYKSAHLQPILQNVLAEAMELLGAQSGSLMLLDPGTNELRISV
                     AHGLPPDVIQRVRVRPGEGIAGWVLAHNRARLLKREERAADSLSSADRLASAISAPLR
                     VERRTIGVLNVSGSRDGDFTRADVRVLMAFAGQAASAIENARLSESLQLKMHQLSALH
                     RVGQALTSTLNLSRVLREVLRRATSILGARCGSIMLLSNDGQELRIASAVGLDRKVVR
                     ETRLAIGQGIAGVVAQSGVSIVLDKGTVDVRSASGRAREHADAAMCVPLRFHGRTIGV
                     INISGPESGDNFGEEHLDFLTTLADQAAIAIENARLYRRLEQRVNLANRELIRANHLL
                     AQEKSKIQAIVDGMADGVLVTNRQGRITFINPTAETMLSIPAAQAVGKGLKHWPAGKS
                     LMALLEEAQQSDGMTIVKEVLVPGEERRVLSANISPQRDERGRPQGFIAVLTDITALK
                     EISDAKTELVSFVSHELRTPITSIQGFAQTMRDAAPGELDRDLQNEFLDIILTESGRL
                     LSMIGEMLDVSRMEAGRGLHLSLAPVDIPGLLQRAVTA"
BASE COUNT          351 a          670 c          623 g          341 t
ORIGIN      
        1 attggcggtc ccttccgcta cgcgatcctc ctcgatatgg tgggagatgc cgatctgcgg
       61 atcccccgag agcgctactc gcacgagcaa gcacgcgcca ttgtggataa agtgtggcag
      121 gccgccgcgg agttgggcta tagccagttt tccgcctcca tcggccccgc catctacgat
      181 gaccacctgc ccctgcttcg caagggaatc ccggccatcg atattatcga ctttgactac
      241 ccgtattggc atacccttca ggacacgccg gataagtgca gccccacgtc actgaagatc
      301 gtgggcgacg tcgtcaggag ggttgtctac gcggaacggt gaacggcatg cagtacaagt
      361 ccgcccacct tcagcccata ctccagaacg tcctcgctga agcgatggaa ctccttggcg
      421 cgcagagtgg ctcattgatg ctcctcgatc ccggaaccaa cgagctgcgg atctctgtcg
      481 cgcatggtct cccccctgat gtcatccagc gcgttcgtgt acgcccaggc gaagggatcg
      541 cggggtgggt gctggcgcac aaccgggcgc gcctcctcaa gcgcgaggaa cgcgccgcgg
      601 actccctctc cagcgcggat cgcctcgcct ccgccatcag cgcgccgctg cgggtggagc
      661 gccgcactat cggggtgctc aacgtcagtg gatcacgcga cggcgatttc acgcgcgcgg
      721 atgtacgcgt gctgatggcc ttcgccggtc aggctgcctc tgcgattgag aacgcgcggc
      781 tatccgagtc gctccagctc aagatgcacc agttgagtgc cctccatcgc gtggggcagg
      841 cgctcacatc caccctcaac ctctcgcgcg tgctccgcga ggtgctgcgc cgggcgacct
      901 cgattcttgg cgcccgctgc gggtcgatca tgctgctgag caatgacggc caagagctgc
      961 gcatcgcctc ggcggtcggc ctggatcgca aggtcgtgcg cgaaacacgc ctggcgatcg
     1021 gccaggggat cgccggcgtg gtcgcccaat ccggggtctc catcgtgctg gacaagggca
     1081 cggttgacgt gcgttcggcc agcgggcgcg cgcgcgaaca cgcggacgcg gcgatgtgcg
     1141 tgcccctgcg ctttcacggg cgcaccatcg gcgtgatcaa catcagcggc ccagagagcg
     1201 gtgacaactt cggcgaggag catctcgatt tcctgacaac actggcggac caggcggcca
     1261 tcgcgattga gaacgcgcgc ctctaccgcc gcctggagca gcgcgtgaac ctcgccaacc
     1321 gcgagctgat tcgcgccaac caccttctcg cgcaggagaa gtcgaagatc caggcgattg
     1381 tcgatggcat ggcggatggc gtgctggtga ccaatcggca gggccggatc accttcatca
     1441 acccaaccgc ggagacgatg ctttccatcc cggcggccca ggccgtgggc aagggcctga
     1501 agcattggcc ggctggcaag agcctgatgg cgctcctgga ggaagcccag cagagcgacg
     1561 gcatgactat cgtgaaagag gtccttgtgc cgggcgagga gcggcgcgtt ctctccgcca
     1621 atatctcgcc gcagcgcgat gagcgcgggc gaccgcaggg cttcatcgcg gtgctcaccg
     1681 acatcaccgc gctcaaggag atcagcgacg cgaagaccga gctcgtctcc ttcgtctcgc
     1741 acgaactgcg cacgccgatc acctcaatcc agggcttcgc ccaaacgatg cgcgacgccg
     1801 caccaggcga gctggaccgc gatctgcaaa acgagtttct cgacatcatc ctcaccgaat
     1861 cagggcgtct cctctcgatg attggcgaga tgctggacgt ctcgcgcatg gaggccggcc
     1921 gtgggcttca cctctccctc gcaccggttg acatcccagg cctcctgcag cgcgccgtga
     1981 ccgcg
//
LOCUS       DAOSCV010000866         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37866_length_1984_cov_2.686884, whole genome shotgun sequence.
ACCESSION   DAOSCV010000866 DAOSCV010000000
VERSION     DAOSCV010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37866_length_1984_cov_2.686884"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1984
                     /locus_tag="PLY56_17170"
     CDS             <1..>1984
                     /locus_tag="PLY56_17170"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOJ23261.1"
                     /translation="TRGDASRLTQKLLRQFKTPEDLLAKARLQALAWLVAAGWLEVRV
                     GLMRNTGGVLHAKFGVVTDRQGDSIAFMGSGNETGQAITQNYEELEVRASWEDPGFTE
                     WYRARFEALWQGNDPDVAAVPLPEAVRKKLIRLAPSEPPKGEPRPDVKALKAAMRWRF
                     IAAAPYLPNGEAACDATMPVDLWPHQRRVVEDTVRAFPAGRLLCDEVGMGKTIEAIAV
                     LRRLLAGRGVRRALLLVPAGLMEQWQSELREKGGLLVPCWKNGILYLPEGGSKAMEAG
                     QALRQYDLLLLSREWARLNNNRELVLTAPEWDLVLMDEAHAARRLKPEEGEFNSGNLL
                     LELLRDLQLRRRTRGILLLSATPMQTQPWEPWDLLATLGVGGKWQVDFADIRAYYEGI
                     EALRNSTLLVGPKARAIARLVVGDEEFPDPPPEAQVLRDDAESLASALAFSPEQPRCA
                     EWLQAGAPLGRRMHRNTRDTLHQYHRMGLLRDKPPQRDVRDVAFDFEDARERECYEAI
                     EHYIDRRYEQLEQEKPGKGFVMTIYRRRAASSPRAFQRSLRRRLERLEAIIQNQSYRP
                     WLGLDEEQLDIRDLGDTDSDEEIDPGLPQTADGAKAEKEEVERLLGMLGSLGNTDSKF
                     ARFQSVLEEVTGDGRSVLVFTEYTDTMVYLRDQLAPI"
BASE COUNT          356 a          594 c          740 g          294 t
ORIGIN      
        1 cacccggggc gacgcgtcgc gcctgaccca gaagctcctc cggcagttca agacgccgga
       61 ggacttgctg gcgaaagcgc ggctgcaggc gctggcgtgg ctggtggcgg ccgggtggtt
      121 ggaagtgcgc gtcggcctca tgcggaacac ggggggcgtg ctgcacgcca agttcggcgt
      181 ggtgaccgac cgccagggcg actccatcgc cttcatgggg agcggcaacg agacggggca
      241 ggcaatcact cagaactacg aggaactgga ggtccgggcg agctgggagg acccgggctt
      301 cacggagtgg taccgcgccc ggttcgaggc gctgtggcag ggcaatgacc cggacgtggc
      361 ggcggtcccg ctgccagagg cggtacgcaa gaagctgatc cggctggcgc ccagcgagcc
      421 gccgaaaggg gaaccgaggc cggacgtgaa ggcgctgaaa gcggcgatgc ggtggcgttt
      481 cattgccgcg gcgccgtatc tcccgaacgg cgaggccgcg tgcgacgcga cgatgcccgt
      541 ggacctgtgg ccgcaccagc gtcgcgtcgt cgaggacacg gtgcgagcgt ttcctgccgg
      601 gcggctgttg tgcgacgaag tcggcatggg caagacgatc gaggcgatcg ccgtcttgcg
      661 ccgtctgctg gcagggcgcg gggtgcgccg cgccctgctt ctggtgcctg ccggcctgat
      721 ggagcagtgg cagagcgagc tgcgcgagaa gggcggtctc ctggtgccgt gctggaaaaa
      781 cgggatcctc tacctcccgg aagggggaag caaggcgatg gaggccgggc aggcgctgcg
      841 ccagtacgat ctgctgctgc tcagccgcga gtgggcacgc ctgaataaca accgcgagct
      901 agtgttgacc gcgccggagt gggatctcgt gctgatggac gaggcgcatg cggcccggcg
      961 gctcaagccc gaagagggag agttcaactc ggggaacctc ctcctggagt tgctccggga
     1021 cctgcaactg cgccggcgca cgcgcggcat cctgttgctg agcgccaccc cgatgcagac
     1081 gcagccgtgg gagccgtggg atcttctggc tacccttggg gtgggcggca agtggcaggt
     1141 ggacttcgcg gacattcgcg cctactacga agggatcgag gcgctgagga acagcacgct
     1201 attggtcgga cccaaggcca gggcgatcgc gcgcctggtt gttggcgacg aggagtttcc
     1261 ggatcccccg ccagaggccc aggtcctccg tgacgatgct gaaagcctgg ccagtgcact
     1321 cgccttcagc ccggagcaac cgcggtgcgc ggagtggctg caggcgggag cgccgctcgg
     1381 gcggcggatg caccggaaca cgcgcgacac gctgcatcag tatcaccgca tgggactgct
     1441 gcgggataaa ccgccccagc gcgacgtgcg cgacgtggcg ttcgactttg aagacgcgag
     1501 ggagcgcgag tgctacgagg ccatcgagca ctacatcgac aggcgctacg agcagttgga
     1561 gcaggagaag cctggcaagg ggttcgtgat gacgatctac cggaggcggg cggccagctc
     1621 gccccgggcg ttccagcgca gcctcaggcg ccggctcgag cggctcgaag ccatcatcca
     1681 aaatcagtcg taccgccctt ggcttggttt agacgaagaa cagctagaca tccgagacct
     1741 gggcgacacg gacagcgatg aggagattga cccgggcctt ccacagacgg cggatggcgc
     1801 caaagcggag aaggaagagg tagagcgtct cctggggatg ttgggtagcc tgggcaacac
     1861 agacagcaag ttcgccaggt tccagtcggt gctcgaagag gtgacgggcg acgggcgctc
     1921 cgtgttggtg ttcaccgagt acaccgacac catggtctac ttgcgcgacc agttagcccc
     1981 catt
//
LOCUS       DAOSCV010000867         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37886_length_1983_cov_2.628631, whole genome shotgun sequence.
ACCESSION   DAOSCV010000867 DAOSCV010000000
VERSION     DAOSCV010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37886_length_1983_cov_2.628631"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1983
                     /locus_tag="PLY56_17175"
     CDS             <1..>1983
                     /locus_tag="PLY56_17175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ23262.1"
                     /translation="ILSDTALFSISQPTELVQAFLSEDGLTIPEDEISEFTQEYLIPL
                     AEQVELAGHFEISREVREDPVKRVYLQEGEEGLVARLAFGYADIELSFAPHAPEYSVS
                     LCPDGSLVRVYRQPEVEDAAWRAMSGFGLKRGDTPGEFVLRKGVTAVDFLIHQIPRLV
                     EAGYQIFGEEAITSVRVNRARPLLSLSVSSGIDWFDLEAVVKFGDLDASWKDVAKAIR
                     RRERYIKLADGSVGMLPSDWIERYRRLLGIVGDEGGKMRVGSHQLTVLDELLRDADDP
                     RVDDEFRRRCERLRDLTRIESQPVPSGFRGELRAYQKAGFDWLHCLHEYGLGGCLADD
                     MGTGKTIQALALLQSLKERGEATAPSLIVMPRSLLFNWQREAARFTPELRTLIYADGQ
                     RDQTLAELSEYDVILTTYGVMLRDIEALSSYRFHYAILDESQAIKNPLSQTARAARRL
                     QADHRLALTGTPVENTTLELWSQFAFLNPGLLGSLEFFRKEFALPIENQRNEETASLL
                     RRLVFPFILRRTKAQIATDLPPRTERVVYCEMEPAQRKAYERFRDYYRATLLGLIEEE
                     GMDRARMKILEGLLRLRQVCNHPRLVDSSFRGSSGKFNELLELLETLRAEGHKALVFS
                     QFTQMLQLVAEALESRGVPYTYLDGSTRDRSARVDE"
BASE COUNT          332 a          627 c          653 g          371 t
ORIGIN      
        1 ggatcctgag tgatacggcg ctcttttcca tctcccagcc cacggagctg gtgcaggcgt
       61 ttctctcgga ggatggtctg acgatccccg aggatgagat ctcggagttc acccaggagt
      121 acctcattcc ccttgccgag caggtggagc tcgcaggaca ctttgagatc tcgcgcgaag
      181 tgcgcgagga cccggtgaaa cgcgtctacc ttcaggaggg ggaagaaggc ctggtggcgc
      241 gcctcgcctt cggctacgcc gatatcgaac ttagctttgc gccgcacgcg cccgagtata
      301 gcgtgtctct ttgcccggat ggcagcctgg tgcgcgtgta tcgccagccc gaggtggaag
      361 acgccgcctg gagggcgatg agcggcttcg ggctgaagcg cggagacacg cccggtgagt
      421 ttgtgctccg caagggcgtg acggccgtcg atttcctcat ccatcagatc cccaggttgg
      481 tcgaagcggg ctatcagatc ttcggagagg aagcgatcac ctccgtgcgc gtcaaccgcg
      541 cgcggccttt gctctcgctc tctgtctcct ccgggatcga ttggttcgat ctcgaggcgg
      601 tggtgaagtt cggcgatttg gatgcctcct ggaaggatgt tgccaaggcg atccggcgtc
      661 gcgagcgcta catcaagctg gcggatggct ccgtgggcat gcttccctca gactggatcg
      721 aacgttaccg gcgtctcctc ggcatcgtgg gcgatgaggg cgggaagatg cgcgtgggct
      781 cgcaccagct cactgtgctc gatgagctgc tgcgcgatgc ggatgacccg cgcgtcgatg
      841 atgagttccg ccggcgctgc gagcgattgc gcgacctgac gaggatcgag agccagcccg
      901 ttcccagtgg ctttcgcggc gagttgcgcg cctatcagaa ggccggcttc gactggctcc
      961 attgcctcca cgagtacggg ctcgggggat gcctggcgga cgacatgggc accggcaaga
     1021 cgatccaggc gctcgcgctt ctccagtcgc tgaaggagcg gggggaggca acggcgccct
     1081 ccctgatcgt gatgccgcgc tcgctcctct tcaactggca acgcgaggcc gcccgtttca
     1141 cgcctgaact gcgcaccctc atctatgctg atggccagcg cgaccagacc ctggcggagc
     1201 tgagcgaata cgacgtgatc ctgaccacct acggggtgat gctgcgcgat atcgaggcgc
     1261 tcagcagcta tcgattccac tacgccatcc tggacgagtc gcaggcgatc aagaacccgc
     1321 tgtcgcagac ggcgcgcgcg gcacggcgcc tccaggcgga ccaccgattg gcgctcacag
     1381 gcacgccggt ggagaacacc accctggagc tctggtcgca gttcgccttc ctcaatccgg
     1441 gcctgttggg gagtctggag ttcttccgta aggagttcgc cctgccgatt gagaatcagc
     1501 gcaacgagga gactgcgagc ctcttgcgcc gcctcgtttt cccgttcatc ttgcggcgca
     1561 ccaaagcgca gatcgccacc gatctcccgc cgcgtaccga gcgcgtcgtc tactgcgaga
     1621 tggagccggc gcagagaaag gcgtacgagc gcttccgcga ctactaccgc gctacccttc
     1681 tcgggctcat cgaggaggag gggatggatc gggcgcgcat gaagatcctg gaaggcctgc
     1741 tccgcctgcg acaggtctgc aatcaccccc ggctggtaga ctccagcttc cgtggctcct
     1801 cgggcaagtt caatgagctc ctggagctcc tggagacgct gcgcgccgag gggcacaagg
     1861 cgctcgtctt ctcgcagttt acccagatgc tccagctcgt ggcggaggcg ctcgagtcac
     1921 gcggggtgcc ctacacctac ctggatggca gcacgcgcga ccggtcggcg cgcgtggatg
     1981 agt
//
LOCUS       DAOSCV010000868         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37937_length_1981_cov_4.242991, whole genome shotgun sequence.
ACCESSION   DAOSCV010000868 DAOSCV010000000
VERSION     DAOSCV010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37937_length_1981_cov_4.242991"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1981
                     /locus_tag="PLY56_17180"
     CDS             89..>1981
                     /locus_tag="PLY56_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23263.1"
                     /translation="MRSILLCALVLGLLGPGWCAGTYTATIEATAQAVLTVAGAWVDF
                     PAFLKLGGETAAFAPESVVVRPAEPAGADPVASRVEESQGTYRLFWEVPAVAQKDAGQ
                     PVTFRIEFGVKPAPGGSPRLPASAVDTLIANGGFEEVTDQGIPVGIAAAIFKRDFRLV
                     AEKEGRALAFNADPEGRGPAFITPWVSVRGGESLAYSFRFRARGAEAHPRYKVILFSY
                     VNYRDAEGKPLPRAHAYTTRLGDTDGWEEVRVTLPVPPAARSTNLEIRNGSQVPYSVS
                     IDDVRVAATTIPQVMAATAPGGQRLSLRVEDENVRRIDLGTATSPVWQGFQALAPEME
                     YTAERGLGFTRLARPLGLDKVRPDPLARDFITAARADLRIDLPDGEYTLWALIGDSQV
                     GSTILTIYRDQLLQINGQEVLKRDVPPAEFFRTTYLRHYADFWHPGMDFYDTFVAPNF
                     EEHFFPVTATGGKLALSWRNLPVAALVVAPKARDAELRAELSRLKAERRRATPIEEIA
                     PPAEEAPAAGVLTAAERKRGFVLFRRPANESIYPTSRPKPEERIAVLQAFATPGEYEP
                     VHFSLYPLRDLGAVRVRATELRARAGGRIPASAVDVRVVRYHFRAAGGRGVAGAYRYQ
                     IAPYILD"
BASE COUNT          309 a          683 c          665 g          324 t
ORIGIN      
        1 caggagggga actcgtgtat agcggcgaag ctggcagtgg tgcctgggac aggctcccgg
       61 ttctggatca ctcgtgagga gagccctgat gagaagcatc ctgctctgtg cgctcgtgct
      121 cggactactc gggccgggat ggtgcgcggg cacttatacc gccactatcg aggcgaccgc
      181 tcaagccgtg ctgacggtcg ccggcgcctg ggttgatttc ccggcattct tgaagttggg
      241 cggggaaacg gccgcgttcg cgccggagtc ggtggtggtc cgacccgcgg aacccgccgg
      301 tgccgatcct gtcgcgtccc gcgtggagga gagccagggg acttaccgtc tcttctggga
      361 ggtgcccgca gtcgcgcaaa aggacgccgg ccagccggtc acctttcgga tcgagttcgg
      421 ggtcaagccc gcgcccggtg gctcgccgcg cctccccgcg tccgctgtag acacgctgat
      481 cgccaacggc ggcttcgagg aggtcacgga ccagggcatt cccgtgggga tcgctgcggc
      541 gatcttcaag cgcgatttcc gcctcgtcgc ggagaaagag gggcgcgcac tcgcgttcaa
      601 cgcggatccg gaaggacgcg ggccggcctt catcaccccg tgggtgtcgg tacgcggggg
      661 cgaatcgctc gcctactcct tccgctttcg tgcgcgcggc gccgaggcgc acccccgcta
      721 caaggtgatc ctcttcagct atgtgaatta ccgcgacgcc gaaggcaagc cgctcccgcg
      781 ggcgcatgcc tacaccactc gcctgggcga taccgatggc tgggaagagg tgcgcgtcac
      841 cctccccgtg ccgccagcgg cgcggagcac caacctggag atccgcaacg ggagccaagt
      901 gccctattcc gtcagcatcg atgacgtgcg cgtcgctgcg actaccatcc cgcaggtgat
      961 ggcggccacg gccccgggtg ggcagcggct cagcctgcga gtggaggatg agaacgttcg
     1021 ccggatcgac ctcggcaccg ccacctctcc cgtgtggcaa ggtttccagg cgctcgcccc
     1081 ggagatggaa tacaccgcgg agcgcgggct gggcttcacg cgcctggccc ggccattggg
     1141 cctcgacaag gtgcgccccg atccgctcgc gcgtgacttc atcaccgcgg cgcgcgccga
     1201 tctgcgcatc gatctccccg acggcgagta caccctctgg gcgctcatcg gtgactcgca
     1261 ggtgggcagc accatcctca caatctaccg cgaccagttg ctgcagatca acgggcaaga
     1321 ggtgttgaag cgcgacgtgc ccccggccga gttcttccgg acgacctacc tgcgccacta
     1381 cgccgatttc tggcaccccg gcatggactt ctacgatacc ttcgtcgcgc caaacttcga
     1441 ggagcatttc ttcccggtga cggcgaccgg tggcaagctg gcgctttcct ggcgaaacct
     1501 tcccgtggcg gcgctcgtcg tggcccccaa ggcgcgagac gcggagctgc gcgccgagct
     1561 cagccggctc aaggcagagc ggcgccgggc cactcccatc gaggagatcg ccccgcctgc
     1621 tgaggaagcc ccggccgcgg gagtgctgac ggccgccgag cgaaagcgcg gctttgtcct
     1681 tttccgccgg ccggcaaacg agagcatcta tcccaccagc cggcccaagc cggaagagcg
     1741 gatcgcggtc ctccaggcct ttgccacgcc cggggagtac gagccggttc acttctcgct
     1801 ctatcctctg cgcgacctag gagcggtgcg cgtccgagcc acggagctgc gcgcgcgggc
     1861 aggcggacgc atccccgcca gcgcagtgga tgttcgtgtc gttcgctacc atttcagggc
     1921 agcaggcggg cgcggcgtgg caggagccta ccgctatcag atcgccccgt acattctgga
     1981 c
//
LOCUS       DAOSCV010000869         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37975_length_1980_cov_2.790649, whole genome shotgun sequence.
ACCESSION   DAOSCV010000869 DAOSCV010000000
VERSION     DAOSCV010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37975_length_1980_cov_2.790649"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..>1980)
                     /locus_tag="PLY56_17185"
     CDS             complement(17..>1980)
                     /locus_tag="PLY56_17185"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ23264.1"
                     /translation="LVAVGAGAQEPLVPNFDFSEGLKGWTAWSSNAQHPWSLDEAVGH
                     AGKPSLRIEARNPSGNVMVMTETDRLKPGERYVIDAWWRTEGVSADASADLRIIFRDA
                     AGAWLTGDDIYPYATQTEGEWTLKRYRIIPPERTVSATVGFWVREATGTFWISDLSIR
                     AIPKGERTYPSMYDYDPDEVTLGPVPLEAFNKLKETDSPFLPRAKRWNQLLFKIAFWQ
                     EDFSRTRRAILYLGRSQDSLGTHGKALNAALADLDRLQQLYGRLYVAQAAAELPTKLD
                     PELERLEKAVAAGHAGLQRFLAGLAPGDAKWAIVPRAPTDQPWWDAEKKRPRYILWNR
                     WSNPAFHDMEELLEMGDCQTLTSGYPITFEKGVAGWQNYLDQWEKLREVGIRRSSLIT
                     HYSLHDKGYLAPEFVKAQGDDPDLRIWDAEGKPMGPKSGVTLFNWLNPKAGAHMEDVL
                     TQMARFFKDRKEYQFYVDSWECAGPYASGVRIGYNPSHQAAFRDYLRARYGSIERLNQ
                     AWNTRYGSFEEIKPAPEKPVAVGDAAAPLAIESQRWAQEAYVDYLKRIRDTIAAVDPT
                     KPVFGEHSGLLDRVLSPRIWESVDILGYHNRGRTTMPVQVWMASLQRVMKKPMGLFEN
                     FWGCQEDHPQRMHEERVMRAQMRRCARLG"
BASE COUNT          323 a          651 c          647 g          359 t
ORIGIN      
        1 ttccggcggc gcgttttcag cccaaccggg cgcagcggcg catctgggcg cgcatcaccc
       61 gctcttcatg catgcgctgg gggtggtcct cctggcatcc ccagaagttc tcgaagaggc
      121 ccatcggctt cttcatcacg cgctgcagcg aggccatcca gacctggacc ggcatcgtcg
      181 tccggccgcg gttgtgatag ccgaggatat cgacggactc ccagattcgg gggcttagca
      241 cccggtccag caggccactg tgctcgccaa acaccggctt ggtggggtcc acggcggcga
      301 tggtgtcgcg gatccgcttc aggtaatcga catacgcttc ctgcgcccag cgctggcttt
      361 cgatagcgag gggtgcggcg gcatccccaa cggcgaccgg cttctcaggc gccggcttga
      421 tctcctcgaa gctgccatag cgggtgttcc atgcctgatt gagccgctcg atggagccgt
      481 agcgcgcgcg aagataatca cggaacgccg cctggtgcga cgggttgtag ccgatgcgga
      541 ccccgctggc atacggtccg gcgcactccc agctatcgac atagaactgg tactccttac
      601 gatccttgaa gaagcgtgcc atctgggtga gcacgtcctc catgtgcgcg ccggccttgg
      661 ggttgagcca gttgaagaga gtgacaccgc tcttggggcc catcggcttc ccctcggcgt
      721 cccagatgcg caggtcgggg tcgtcgccct gggccttcac gaactcgggg gcgaggtagc
      781 ccttatcgtg caggctgtag tgcgtgatga gagaagagcg ccggatgcca acctcccgca
      841 gcttctccca ctggtccagg tagttttgcc agccagccac gcctttctcg aaggtgatgg
      901 ggtagcctga ggtgagcgtc tggcaatccc ccatctcaag aagctcctcc atgtcgtgga
      961 aggccgggtt gctccagcgg ttccagagga tgtagcgcgg gcgcttcttc tcggcgtccc
     1021 accagggctg atcggtgggc gcccgcggca cgatggccca cttcgcatct cctggtgcca
     1081 ggccggcgag gaagcgctgc agccccgcgt gcccggcggc taccgccttc tccaggcgct
     1141 ccagctccgg gtcgagcttg gtcggaagct ccgctgcagc ctgggccacg tagagccggc
     1201 catacagctg ctggagccgg tcgagatccg cgagcgccgc gttcagcgcc ttcccgtgcg
     1261 tgccgaggga atcctgcgag cgccccaggt agaggatggc gcgccgggtg cgcgagaagt
     1321 cttcctgcca gaaagcgatc ttgaagagaa gctgattcca ccgcttcgcc cgcggcagga
     1381 agggcgagtc cgtctccttc agcttgttga acgcctcgag cggcaccggg cccagggtga
     1441 cctcgtccgg atcatagtca tacatcgagg ggtaagtgcg ttcccccttg ggaatggccc
     1501 ggatcgagag gtccgagatc cagaaagtgc cggtggcctc gcgcacccag aagccgacgg
     1561 tggcggagac cgtgcgctct ggcggtatga tgcggtagcg cttcagcgtc cattcaccct
     1621 cggtctgggt agcataagga tagatatcat cgccggtgag ccaggctccc gccgcgtctc
     1681 ggaagatgat gcgcagatcc gcgctcgcgt cggcactcac gccctcggtg cgccaccaag
     1741 catcgatcac gtaacgctcg cccggcttca ggcggtcggt ctcggtcatc accatcacat
     1801 tgccggaggg gttgcgcgcc tcgatgcgca gggagggctt gccagcgtgc cccactgcct
     1861 catcgagcga ccagggatgc tgcgcgttgc tcgaccaggc cgtccagccc ttcagcccct
     1921 cggagaaatc aaagttggga accagcggtt cctgagcgcc agcgcccacc gcgacgagcc
//
LOCUS       DAOSCV010000870         1979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_37993_length_1979_cov_3.758316, whole genome shotgun sequence.
ACCESSION   DAOSCV010000870 DAOSCV010000000
VERSION     DAOSCV010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37993_length_1979_cov_3.758316"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1569
                     /gene="glmM"
                     /locus_tag="PLY56_17190"
     CDS             211..1569
                     /gene="glmM"
                     /locus_tag="PLY56_17190"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ23265.1"
                     /translation="MKPLKLSILGVRGIVGEALTPELVVTFAQAFGTYIEGGPIFVSR
                     DTRPSGVMIASSVIAGLLATGYEVIDLGVCPTPAMELAVRRHNGAGGVAITAGHNPSD
                     WNALKFVAGTGRLLNAYQGEELLDVFHQGEFARARWDGLKPLRHQAHAVPEYVEALLE
                     QVDREAIRKRRFKVAVDCCNGACVDATMRFLTALGCNVLAINDEVERPFPHDPEPNPD
                     NMSQLRAMVKASGADIGFAQDADGSRLGVVTEKWDALSEEYTLCLIAEMLLQSGDTLV
                     TNSCTTRAVEDIAARRGARTIRTKVGEPHIVETMLFHDAALGGEGSGGIVLPRMHHVF
                     DSTAAIAYILDYLARTDRPISALAGSIPRYYMVKERVPCPPERAPAVMARLREMVDER
                     PDLGNVDFTDGIRLERGRSWVHLRPSMTEPVLRIIAESETAGEARSLVDEWGGHVRSF
                     LL"
     gene            1667..>1979
                     /locus_tag="PLY56_17195"
     CDS             1667..>1979
                     /locus_tag="PLY56_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ23266.1"
                     /translation="MKSVTNLKISISGVRAVVGDTLSPLLMVRFAQAFGTWTGSGTVV
                     VGSDTRTSGEMVRHAVLSGLASAGCRVVDVGICPVPTVQVMVEHLGAAGGVAITASHN
                     PV"
BASE COUNT          330 a          632 c          665 g          352 t
ORIGIN      
        1 tgaaagggat cgccaaggtg gacccgcaga cgcacgcgat ccagatggtg gcagagtcgc
       61 ccgtgcgcat cgccgctggc ggcgatatcg tggacggccg gctctacttt gcctacggct
      121 cgcacgtcta cagcttcaag ttgccctagc aagcgcattt ccggcccggg gaaggattca
      181 ccccgggccg ggtcgaacct gggcaacaac atgaagccgc ttaagctatc gatactgggt
      241 gttcggggga tcgtcggtga ggcgcttacc cccgagttgg tagttacctt cgcgcaagcc
      301 ttcggtactt atatcgaagg ggggccgatc tttgtcagcc gcgatacgcg tccctccggc
      361 gtgatgatcg cctcttccgt catcgccggc ctgctggcga ccggctatga ggtgatcgat
      421 ctcggcgtct gccccactcc ggcgatggag ctggccgtgc gccggcacaa cggcgccggc
      481 ggggtggcga tcacggcggg gcacaacccc tcggactgga acgcgctcaa gttcgtcgcg
      541 ggaaccggcc gcctcctcaa tgcctaccag ggcgaggagc tgttggatgt cttccatcaa
      601 ggcgagttcg cccgcgccag atgggacggc ctgaagcccc tgcgccacca ggcgcacgcc
      661 gttccagagt acgttgaggc gctcttggag caggtggatc gggaggcgat ccggaagcgc
      721 cgcttcaaag tggcggtgga ctgctgcaat ggcgcctgcg tggacgccac catgcgcttc
      781 ctcaccgcgc ttggatgcaa cgtgctcgcg atcaacgatg aggtggagcg ccccttcccc
      841 cacgatccgg agccgaaccc tgacaacatg agccagctcc gcgccatggt caaggcgagt
      901 ggagccgaca tcgggttcgc gcaggacgct gacggcagcc gcctcggcgt cgtcacggag
      961 aagtgggatg ccctctccga ggagtacacg ctctgcctca tcgcggaaat gcttctccaa
     1021 agcggtgata cgctggtgac taactcctgc accacccgcg ccgttgaaga tatcgccgcg
     1081 cgccgcggcg cccgcacgat cagaacgaag gtgggcgagc cgcatatcgt cgagacgatg
     1141 ctcttccacg acgccgccct cggcggcgag gggagcggag gcattgtcct tccgcgcatg
     1201 caccacgtgt ttgactccac ggcggcgatt gcctacatcc tcgactacct ggcgcgcact
     1261 gataggccga tctccgcgct ggcgggaagc atcccgcgct actacatggt caaggagcgc
     1321 gtgccctgtc cgcccgagcg cgcgccggcg gtgatggcgc gcctgcgtga gatggtggat
     1381 gagcgtccgg atctgggcaa tgtggacttc actgacggaa tccgcctcga gcgtggccgg
     1441 tcgtgggtac acttgcgccc ctcgatgacg gagccggtgc tgcgcatcat cgcagaatcg
     1501 gaaaccgccg gagaggcacg ctccctcgtc gatgaatggg gcggtcacgt ccgcagtttc
     1561 ttgctgtgag ggtatcctcg cagccgatcc gtggggaggg tagcacccgc cgtttaggaa
     1621 aggcccatcc ccacgaatag ctgtcgtctt gaagtaggga aagagcttga agagcgttac
     1681 gaatctcaag atcagtatct ctggggtgcg ggcggtcgtc ggtgacacgc tctcgccctt
     1741 gctgatggtg cgcttcgcgc aggctttcgg cacctggacg ggatccggca ccgtcgttgt
     1801 cggcagcgac acgcgtacct ccggcgagat ggtgcgccac gccgtgctct ctgggctggc
     1861 ctccgccggc tgccgcgtcg tggatgttgg gatctgcccg gtgccgacgg tgcaggtgat
     1921 ggtagagcac ctcggcgcgg cgggcggggt cgcgatcacc gccagccaca acccggtcg
//
LOCUS       DAOSCV010000871         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38045_length_1977_cov_4.349636, whole genome shotgun sequence.
ACCESSION   DAOSCV010000871 DAOSCV010000000
VERSION     DAOSCV010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38045_length_1977_cov_4.349636"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1457)
                     /locus_tag="PLY56_17200"
     CDS             complement(84..1457)
                     /locus_tag="PLY56_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ23267.1"
                     /translation="MEIIAEAGIQTGDNNAGRPSFPGPASAAMIAELDRYVLADVRPF
                     VIDLARCHGMWLATIDGQEIFDWAGYYASKLIGHNHPGLYEPEYVERLVRAANNKVAN
                     PDFLTPECLEYYRTLHRLAPECMRNPHLEVYAVNSGAEAVENMMKYFINLYHQRHGGQ
                     RPPDRTHRFLYFEQAFHGRTIFALSVTQTVDPVATKDFHGFIPGNVQVPFPAIDTSLP
                     KSENRRRTEEALQTVAALLERFQGEVVGIVTEPIQGAGGHRCAEPEFFQGLSRLAHEH
                     SVFLGFDEVQTAGGPTGALFMADQLDLPYPPQAIATAKKFGAGVVYMLHPMEDRGVLD
                     STWGGSLADMVRFVQEMKIVEREGLIPAAARKGELLADGLRALSCRFPELVRNVRGMG
                     LYQGFSLPTPEVRARLIQTALQQENLLLLGAGCRSIRTRPNLSVTEEEIGLFLEKLER
                     CLTALAG"
BASE COUNT          343 a          625 c          690 g          319 t
ORIGIN      
        1 tcccttcggg agcgtggcat ggtctcccgc gtgacgacgg aagaggctgg agagggccgg
       61 tgcccgggct atccggcatg gggtcagccg gcgagggcag taaggcagcg ctccagcttc
      121 tccaggaaga ggccgatctc ctcctcggtc acgctgaggt tcggacgggt gcggatggag
      181 cggcatcctg cgccaagcag gagcaggttc tcctgttgaa gagcagtttg gatcagcctg
      241 gcgcgcactt cgggcgtggg gagcgagaag ccctggtaga gccccatccc gcgcacgttg
      301 cgcaccagct ccgggaaccg gcacgagagc gcgcgcaggc cgtccgccag gagctcgccc
      361 ttgcgggccg ccgccgggat cagcccctcg cgctccacga tcttcatctc ctggacgaag
      421 cggaccatat cggccaggct ccctccccag gtggagtcga ggacgccgcg atcctccatc
      481 gggtggagca tgtagaccac accggcgccg aacttcttcg ccgtggcgat cgcctggggc
      541 ggatagggga gatcgagctg gtccgccatg aagagcgcgc cggtcgggcc gcccgcggtc
      601 tgcacctcgt caaacccgag gaagaccgag tgctcgtgcg ccaggcgact gaggccctgg
      661 aagaactcag gctcggcgca acgatgcccg cccgcgccct ggatcggctc ggtgacgatg
      721 ccgaccactt cgccctggaa gcgctcgagc agcgcggcga cggtctgaag cgcctcctcg
      781 gtgcgcctcc ggttctccga cttcggcagc gacgtgtcga tagcagggaa cggcacctgc
      841 acattccccg ggatgaagcc gtggaaatcc ttcgtcgcta ccggatcaac cgtctgcgtc
      901 acactgagcg cgaagatagt gcgcccgtgg aatgcctgct cgaaatagag gaagcggtgg
      961 gtccgatccg gtggccgctg cccgccgtgg cgctggtggt agaggttgat gaagtacttc
     1021 atcatattct caaccgcctc ggcgccagaa ttgacggcat acacctcgag gtgcggattg
     1081 cgcatgcatt caggggccag acgatggagc gtgcggtaat actccaggca ctcgggcgtg
     1141 agaaagtcgg ggttggctac tttgttgttg gcggcgcgca ctaggcgctc cacgtactcc
     1201 ggctcgtaca ggcccgggtg gttgtgcccg atcagcttcg atgcgtagta gcccgcccag
     1261 tcgaagatct cctgcccatc gatggtggcg agccacatcc cgtggcaccg cgccaggtcg
     1321 atgacgaagg ggcgcacatc ggccaagaca tagcggtcca gctccgcgat catcgccgcg
     1381 ctcgccggcc ccggaaaaga cggccgcccc gcgttgttat cccccgtctg aatgcctgcc
     1441 tccgctatga tctccatgcc aacagtatac cctgccggtg acttgcgatc aagcagaagc
     1501 cgcgctctct ccaggggcct gggggagaga tgatcggctc gcgacgcccc ggcggagtct
     1561 gggaggagat tggagggagg ggcgtcaaac agaaaagcct gggcttccga ggtaaggaat
     1621 ctgaagccac ggagtcagcg cgtatcccgg cgcgcgggga tcaatccccg cgctgcttgc
     1681 aagcgaagcc ctgccgggct gggttgagga tactcccaga tgtctcaaaa cggcggtggc
     1741 tgggagacgg agccccgaag gggctttgcc ggaaagtagc gcggagcttc gagctccgcg
     1801 cgcgtgggtg ttgcgcaacc cggcgcgcgg tgctgcaagc accgcgctgc ttgcaagcga
     1861 agccctgccg ggctgggccg ggctacattc gcggcgcaag cgcaatggtg cgggctgggg
     1921 ggcggagccc cgaaggggct ttgccggaaa gtagcgcgga gcttcgagct ccgcgcg
//
LOCUS       DAOSCV010000872         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38059_length_1977_cov_2.943288, whole genome shotgun sequence.
ACCESSION   DAOSCV010000872 DAOSCV010000000
VERSION     DAOSCV010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38059_length_1977_cov_2.943288"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLY56_17205"
     CDS             <1..346
                     /locus_tag="PLY56_17205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ23268.1"
                     /translation="AARLPFTPTELDDVVLAVGEAAANAVRHGLRAGARGVIHVRCRA
                     DRTGFCVEITDPGDGFDPERVQRPKAGVWQEGGLGIYLMRRIMDAVSYTFDARGTTVR
                     LIKRASSAAPAQ"
     gene            636..1346
                     /gene="lipB"
                     /locus_tag="PLY56_17210"
     CDS             636..1346
                     /gene="lipB"
                     /locus_tag="PLY56_17210"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOJ23269.1"
                     /translation="MATLVIDLGRIDYGECLEIQRKLATSRAAGTVPDLLLLCEHPPV
                     VTIGRGGGWEHVGEAVARHGVPVIKVERGGDVTYHGPGQIVGYPLLALEGEERDLHRY
                     LRALEEVVIRAAGDFGIAAGRRSGLTGVWVGNDKLCAIGVAVRRWVTLHGFALNMNTD
                     LQPFEWIVPCGLHGYGVTSMERLLGTPQDPAAVRAAVTRHFAAVFGRRVRPARLDDLD
                     ADAPVPRDCASERSGYPW"
     gene            1340..1645
                     /locus_tag="PLY56_17215"
     CDS             1340..1645
                     /locus_tag="PLY56_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOJ23270.1"
                     /translation="MVTMEIAVHPIGTGNASLSDYVADVIEVAKRRGVKYEITGMGTN
                     LEGDLNTLFDIAREMHEASFRRGAPRVFTLIKVDDRRDKDLTLEYKRRRAAEKVGDA"
     gene            1646..>1977
                     /locus_tag="PLY56_17220"
     CDS             1646..>1977
                     /locus_tag="PLY56_17220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017815509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOJ23271.1"
                     /translation="MGDEGLARLVPEAARRTLVMGILNVTPDSFSDGGQHASVETALA
                     HARRLLDEGADIIDVGGESTRPGADPVAAEEELRRVIPVIEGIRRFSQVPISVDTTKA
                     VVARAAAAA"
BASE COUNT          305 a          619 c          721 g          332 t
ORIGIN      
        1 ggccgcgcgc ctccccttca ccccgacgga gctggacgac gtcgtgctcg ccgtgggcga
       61 agcggccgcg aacgccgtgc gccatgggct tcgcgcggga gcccgcggcg tgattcacgt
      121 gcgctgccgg gcagatcgca ccggcttctg cgtcgagatc accgatccgg gggatggatt
      181 cgacccggag cgggttcagc ggccgaaggc gggcgtctgg caggaaggcg gcttgggtat
      241 ctacctgatg cggcggatca tggacgccgt gagctacacg ttcgatgccc gtggcacgac
      301 cgttcgtctc atcaagcgcg cctcgtccgc ggcccccgcg caataggcga gatcgacccc
      361 tcggatccta ctccctgacc accccgcatc cagcgtcccc gcattcccgt gcccttgaaa
      421 tccggatgaa cagggctcct cgcgcgccgc ggcgaatcta ccctgtgctg ggggcaagcg
      481 ctgcgcgatg acccgaaccc gaccggccag acgcgggtag cgacgcccaa tcgggcggat
      541 gccctgcggt tcggacggcc tggcaccttc gcagggatcg gcagggcacg gttcggcgcg
      601 cttgcggaac cactggcggc aaaggacggg gcggcatggc gacgctcgtg atagacctgg
      661 ggcggattga ctacggcgag tgcctggaga ttcagcggaa gctggccacg tctcgcgcgg
      721 cgggcaccgt gccggatctc ttgctcctct gcgagcatcc gcccgtagtt accatcggac
      781 gaggcggcgg ttgggagcac gtgggggaag ccgtcgctcg ccatggggtg ccggtgatca
      841 aggtggagcg gggcggcgat gtcacctacc acggccccgg gcagatcgtt gggtatcccc
      901 tcttggcgct tgagggtgag gagcgcgacc tccatcggta tttgcgtgcc ctggaggagg
      961 tggtgatccg cgcggcgggt gacttcggga tcgctgccgg aaggcgttct ggtttgaccg
     1021 gcgtgtgggt cgggaatgat aagctgtgcg ccattggggt ggcagtccgg cgctgggtca
     1081 ccctgcatgg cttcgcgctc aacatgaaca ccgaccttca gccgttcgag tggatcgtcc
     1141 cgtgcgggct gcatggctat ggcgtcacct ctatggagcg cctgttgggc accccgcagg
     1201 atcctgccgc cgtccgcgcg gcggtcaccc ggcacttcgc ggccgtcttt ggccggcgag
     1261 tgcggcctgc ccgcctggac gatctcgatg ccgacgcgcc cgttccccgc gactgcgcgt
     1321 cggagaggag tggatatcca tggtgacgat ggagattgcc gtacacccga ttggaacggg
     1381 gaatgccagc ctcagcgact acgttgccga cgtgatcgag gtggccaagc gccgcggagt
     1441 gaagtacgag atcaccggca tgggcaccaa cctggaaggt gacctgaata ccctcttcga
     1501 cattgctcgc gagatgcacg aggcgagctt ccgtcgaggc gcgccgcgcg tcttcacgct
     1561 catcaaggtg gatgaccggc gcgataagga tctgactttg gagtataagc ggcgccgtgc
     1621 cgcggagaag gtgggagacg catagatggg tgacgaggga ctcgccaggc tggtgccgga
     1681 ggccgcgcgc cgcacgctgg tcatgggtat cctgaacgtg accccggatt ccttcagcga
     1741 cgggggacag cacgcgagcg ttgagacggc gctggcccat gcccggcggc ttctggacga
     1801 gggtgcggat atcatcgacg tggggggcga gtcgacgcgc ccgggagcgg atccggtcgc
     1861 tgccgaagag gagctccggc gcgtgatccc ggtgattgag gggattcgcc gcttctcgca
     1921 ggttccgatc tccgtggata ccactaaagc ggtcgtggcc cgcgcggcgg cggcggc
//
LOCUS       DAOSCV010000873         1973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38190_length_1973_cov_2.297706, whole genome shotgun sequence.
ACCESSION   DAOSCV010000873 DAOSCV010000000
VERSION     DAOSCV010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38190_length_1973_cov_2.297706"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLY56_17225"
     CDS             complement(<1..868)
                     /locus_tag="PLY56_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23272.1"
                     /translation="MQREVGDPRRDMVDPGSSSSGPGEERSGGRMATGRALALGLLFL
                     VGAGFWIRASEIVASVVYVTESIPAIPAVPALLILLLANHLLRRSGRRLTPGELVFAF
                     CFVCVGAMTFGVGVVRFLLALITAPFYFAQTGNRLAEAQSHLPKWAAVHDPETVRALY
                     ESLRGAPIPWHLWGVPVLAWTGFLLALWVTLLCLVLLMYRPWIEREKLSFPLLQLPLE
                     LAEVKPRNPAEPAFLRNPMMWAGFGLAVAYNAVHIAHALWPTFPGFQRYFSFAPFSTP
                     PWNAVGTLTFHYR"
     gene            complement(899..>1973)
                     /locus_tag="PLY56_17230"
     CDS             complement(899..>1973)
                     /locus_tag="PLY56_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23273.1"
                     /translation="LAFCIRGVMAIMAVEYKESLGPYGTPSPIFAHLGMGAMIVLVFS
                     GLWYARPHLREVWRTITGRPGGADDSGEVLSYRMAFIGMCAGVLCMGAWLWMAGMPLP
                     VIPVLLVAAFVLFIGLTRVVVESGLAEAVASTTAMGFSVSSLGTAAFGPKGLVALGLC
                     YVWAGDLRTLMMTSTANSLRMGELARPSRRPLVGAIFLAILIGLFGSLAMTLFLAYKK
                     GGGLNLNNWFFVNGPQSPWRYVADRMNNPTGVSGSGLLLESLGAGVMLFLTIMRQRHV
                     WWPFHPIGFAVGTTWIMDQIWLSALICWALKLGIVRFGGLRAFRTARPIFLGLILGQF
                     TCNGMWLIIDSFTGMKGNQIFWI"
BASE COUNT          416 a          627 c          669 g          261 t
ORIGIN      
        1 gccggtagtg gaaggtgagc gtgcccacgg cgttccaggg gggcgtggaa aagggggcaa
       61 agctgaaata gcgctgaaag cccgggaagg tgggccagag cgcatgggcg atatgcaccg
      121 cgttgtaggc cacggccagg ccaaacccgg cccacatcat cgggttgcgc aggaaggccg
      181 gctcggccgg gttgcggggc ttcacctcgg ccagctccag aggaagctgc agcagcggaa
      241 aggagagctt ctcccgctcg atccaggggc ggtacatcaa cagcaccagg cagaggagag
      301 tgacccagag agcgagaagg aagccggtcc aggccaacac aggcactccc cagaggtgcc
      361 acgggatcgg cgcgccgcgc agcgactcgt agagggcgcg cactgtctcg ggatcatgca
      421 cggcggccca cttcggcaga tggctctgtg cctccgcaag gcggtttccc gtctgggcga
      481 agtagaaggg cgcggtgatc agggcgagaa ggaagcggac cacgcccaca ccgaaggtca
      541 tcgcgccaac gcagacaaag cagaaggcga agacgagctc tcccggagtg agccggcgcc
      601 cgctgcggcg caacaggtgg ttggcaagca ggaggatgag gagtgcgggg accgcgggaa
      661 tggctggaat actctcggtg acgtacacca ccgatgcgac gatctcagat gctcgaatcc
      721 agaacccggc gccgacaagg aagagcagcc caagcgccaa cgcccggccc gtcgccatcc
      781 tgcccccgga ccgctcctcg cccggcccag aactactcga ccccggatct accatgtcgc
      841 gccgcggatc acccacttct ctttgcactc gccatccttt cgtgccgttc acgacgcctc
      901 agatccagaa gatttggttg cccttcatgc cggtgaagga gtcgatgatc agccacatgc
      961 cgttgcaggt gaactggccg aggatcaggc ccaggaagat cgggcgcgcg gtgcggaacg
     1021 cccggaggcc gccgaagcgg acgatgccca gcttcagcgc ccaacagatg agagcggaga
     1081 gccagatctg gtccatgatc caggtggtgc ccacggcgaa gccgatcggg tggaagggcc
     1141 accagacgtg ccgctggcgc ataatggtca ggaaaagcat gaccccggca ccgaggctct
     1201 ccagcagcaa cccgcttccg gagacgccgg tggggttatt catcctgtcg gcgacgtagc
     1261 gccagggact ctgcgggccg ttcacaaaga accagttgtt caggttgagg ccgccgcctt
     1321 tcttgtaggc caggaagagc gtcatcgcca gggagccgaa gaggccaatc agaatggcca
     1381 ggaagatggc gccaaccagt gggcggcggc taggacgggc cagctcaccc atgcgcaggc
     1441 tgttcgcggt cgatgtcatc atcagcgtgc gcaggtcgcc tgcccacacg tagcaaaggc
     1501 cgagcgccac caggcccttc ggtccgaagg cggcggtgcc cagactggag acggagaagc
     1561 ccatcgccgt ggtggatgcc acggcctccg cgagcccgct ctctaccact acgcgcgtca
     1621 gcccgatgaa gagcacgaac gcggcgacga gcaggactgg gatcacgggc agcggcattc
     1681 ctgccatcca gagccaggca cccatgcaga ggacaccggc gcacatgccg atgaatgcca
     1741 tgcgatagga gagcacctcg cccgagtcat cggcgcctcc ggggcggccg gtgatggtgc
     1801 gccacacctc tcgcaggtgg gggcgcgcat accacaggcc tgagaagacg agcacgatca
     1861 tggcgcccat ccccaagtgg gcgaagatcg gcgacggggt gccatacggc cccaggctct
     1921 ccttatactc gacggccatg atcgccatca cgccgcggat gcagaaggcg agg
//
LOCUS       DAOSCV010000874         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38213_length_1972_cov_2.892540, whole genome shotgun sequence.
ACCESSION   DAOSCV010000874 DAOSCV010000000
VERSION     DAOSCV010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38213_length_1972_cov_2.892540"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="PLY56_17235"
     CDS             complement(<1..1096)
                     /locus_tag="PLY56_17235"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ23274.1"
                     /translation="MDAGARDQGRALVALARLPDRKLREDAIEQAIQEVLAPLGGLPR
                     FISPGAKVLLKPNQTLFRAALSGSTTSPRLVRTLIRLCFRAGAKEVWVAEASGHAQRS
                     RQVMALTGMAQAVKETGAHLIYLDEIAEKVYDFGEDAGDLRYMPAPEIMERADVIINL
                     PKAKTHFIDPISGACKNWVGVIPMGYRLYLQRCGAAYYRGNALYLRRFPPALNLMDGI
                     VAGEGQGPGQNHPFWWGWLLASEDPVALDVTLCRLFGLEWGRLRMAQEAAKLGAGVFD
                     PSRITIAGAAFQDACVHVRPADPSVHRYPCRVIVGEYAPMEATLGHWKTIADAWLEND
                     LWRLFTSRGTPTFLFGRAEDPEFERHVKDGP"
     gene            complement(1378..>1972)
                     /locus_tag="PLY56_17240"
     CDS             complement(1378..>1972)
                     /locus_tag="PLY56_17240"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ23275.1"
                     /translation="APPVMYTFSGGFLPGTVNISPQLFALNLMEICHSLVLQGFRNIV
                     LLLGHGGSENTRAAQEAAENFQRLNPQLEGVTIAVVPFWELSPTMMEAFEQGDYHAAR
                     IETSLMLFWHPELVRMEKATRDNPELVARMAADPDAYLVKEKRVDHPFVVARLTQDPQ
                     MEVGVMGDFAGASAELGRTVAEEATRGLVEFLSRLEG"
BASE COUNT          343 a          644 c          645 g          340 t
ORIGIN      
        1 aaggaccgtc cttcacatgc cgctcgaact cagggtcttc cgctcttcca aaaagaaagg
       61 tcggcgtgcc tctcgaggtg aagagacgcc acaggtcatt ctctagccaa gcatcggcaa
      121 tcgtcttcca atgcccgagg gtggcctcca ttggcgcata ctcgccaaca atcacgcggc
      181 agggatagcg atgcacactc gggtctgctg ggcgcacgtg cacgcatgcg tcctggaagg
      241 cggcgcccgc gatggtgatt cgcgatgggt cgaagacgcc ggcgcccagt ttcgccgctt
      301 cctgggccat gcgcaggcgt ccccactcca ggccgaagag ccggcagagg gtgacatcca
      361 gggcgaccgg gtcctccgaa gccagcagcc aaccccacca gaagggatgg ttctggcccg
      421 gcccctggcc ttcaccggca acgatgccat ccatgagatt cagcgccggc gggaaccggc
      481 gcagataaag cgcattcccg cggtagtagg ccgcgccaca tcgctgcagg tagaggcgat
      541 agcccatcgg gatgacgccc acccagttct tgcaggcgcc ggagatggga tcgatgaagt
      601 gcgtctttgc cttgggcagg ttgataatga catcggcgcg ctccatgatc tcgggcgccg
      661 gcatatagcg caggtcaccg gcatcctcgc cgaaatcgta caccttctcg gcgatctcat
      721 cgaggtagat caggtgcgct ccggtctctt tcaccgcctg cgccatcccg gtgagcgcca
      781 tcacctggcg gctgcgctgg gcatgcccgg atgcctcggc gacccacact tcttttgcac
      841 ctgcgcggaa gcagaggcgg atgagcgtgc gcacgaggcg tggtgacgtg gtcgagccgg
      901 agagcgcggc gcggaagagc gtctggtttg gcttcaggag caccttggcg cccggcgata
      961 tgaatcgggg caggcccccg agcggcgcga gcacctcctg gatcgcctgc tcgatcgcgt
     1021 cttcccttag cttccggtcc ggcagacgtg ccagtgcgac cagcgcgcgg ccctgatctc
     1081 tggctccggc atccatgcat tccctcccgc ggcaggctgg cccggatgag aggagacacg
     1141 cgaagccccc tcgggccatc cctgatgggc gaacctttga ctgctttacc caccgggccc
     1201 tcgcttcaaa cagcgagagt tattccgatc gcgtgtcggc tatgccgcag ggggataggg
     1261 gcagggatgt ccatcgagtg ggggcgtcgg gggccaccgc gatcctctca ctgatcggag
     1321 tagcggcagc acgccggcag gacgggtgct ctggatgcac gcgtcctgcc ggggatctca
     1381 tccctccagg cgcgacagaa actccaccag gccgcgcgtc gcctcctcgg ccacggtacg
     1441 ccctagctca gcgctggcgc ccgcgaagtc gcccatgaca cccacctcca tctgcgggtc
     1501 ttgggtcagc ctggcgacca cgaaggggtg atccacgcgc ttctctttca ccagataggc
     1561 atcgggatcg gcggccatgc gcgccacgag ctccgggtta tctcgcgttg ccttttccat
     1621 gcgcacgagt tcggggtgcc agaagagcat gagcgaggtc tcgatgcgcg cggcgtgata
     1681 gtcgccctgt tcaaacgcct ccatcatcgt cggcgaaagc tcccagaagg ggacgacggc
     1741 gatggtgacg ccttcgagtt gcgggttgag ccgctggaaa ttctccgccg cctcctgggc
     1801 ggcgcgcgtg ttctcgctgc cgccgtggcc caggagcagg acgatgttcc ggaagccctg
     1861 aaggacgagc gagtggcaga tctccatcag gttcagggcg aaaagctgcg ggctgatgtt
     1921 gaccgtgccg ggaaggaacc ctcccgagaa ggtgtacatc accggcgggg cg
//
LOCUS       DAOSCV010000875         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38322_length_1968_cov_2.788813, whole genome shotgun sequence.
ACCESSION   DAOSCV010000875 DAOSCV010000000
VERSION     DAOSCV010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38322_length_1968_cov_2.788813"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PLY56_17245"
     CDS             <1..1729
                     /locus_tag="PLY56_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ23276.1"
                     /translation="RTRGGLGFLAKVAGLVLSGVLIRFVTKVADMGTPMAGALMVILL
                     LVIMPGTAWMRRKDGHRAIRPLLVWAAVMGFASLLVPGNFRFEFAPSVDRRQFEVTID
                     YEVGTSLDVTDATARQIEAALLDRNLYPEVKSLFTTAGASRTGSGLGAFGSDTDYATI
                     SGELVDYVPGKTRRTDEIVRDINARFADLPGVTVRASSSGGITHGKPVQIEVTGTDQE
                     QIVTAAYQVAQVLKETPGAFAVELSSREGRPELQARIDRERAAQYGVSVAQVATALRA
                     SLQGDTTTKYREEGDEYDIRVTLPEAQRDRTDRIPNLVVATTATGRPVYLYEVATLDT
                     AGGPTKLERSERQRTVTVSCELAEGVALGNAQLAINERLAKLQTNGVSVRWVGQAEFM
                     EESNRDMMSALGLSVLLVFMLMAALFESLLSPLIIMLSVPQAMAGAFFALTFTGKSLS
                     MPSMIGMIMLVGLVTKNAILLVDYTNTLRNEHGMERRAALLQAGPTRLQPILMTTLAM
                     IGGMLPTAMALTKGSEMRQPMAIAVIGGLALSTFLTLLMVPVFYEVMDDLGERFARAK
                     EAVIRLLRV"
BASE COUNT          327 a          594 c          695 g          352 t
ORIGIN      
        1 gcgcactcgc ggtgggctcg ggttcctggc caaagtggcc ggactggtgc tctcgggagt
       61 gctgatccgg ttcgtcacca aagtggcgga tatgggcacc ccgatggcgg gcgcgctcat
      121 ggtgatcctc ctgttggtca tcatgcctgg aacggcctgg atgcggcgca aggatgggca
      181 tcgggctatc cgcccactcc tggtgtgggc cgcggtgatg ggctttgcca gtctcctggt
      241 gccgggtaac ttccggtttg agttcgctcc cagcgtggac cggcgccagt tcgaggtgac
      301 tatcgactat gaggtgggca cctccctgga tgtgacggat gcgaccgcgc gccaaattga
      361 ggccgcgctg ctcgaccgca acctgtatcc agaggtgaaa agcctcttta cgaccgctgg
      421 cgcgtcgcgc accggtagcg gcctgggcgc ctttggcagc gataccgatt acgcgacgat
      481 cagcggcgag ctggtcgact acgtgcctgg gaagacgcgg cgcaccgacg agatcgttcg
      541 cgacatcaac gcgcgcttcg cggatctccc gggcgtgacg gtgagggcga gctcctccgg
      601 cggtatcacg cacggaaagc cggtgcagat tgaggtcacc ggcaccgacc aggagcagat
      661 cgtgacggcg gcgtaccagg tggctcaggt gctgaaggag acgccgggcg ctttcgccgt
      721 cgagctctcc tcgcgcgagg gccggcctga gcttcaggcc cggatcgatc gcgagcgtgc
      781 cgcacagtac ggcgtgagcg tcgcacaggt ggctaccgcg ttgcgcgcca gcctccaggg
      841 cgacaccacc accaagtatc gcgaggaagg cgacgagtac gacatccggg tgacgctgcc
      901 ggaagcacag cgcgaccgga cggaccgcat ccccaacctg gttgtggcga ccacggcgac
      961 gggccggccg gtatacctgt atgaggtggc cacgctggat accgccggtg gcccgacgaa
     1021 gctggagcgg agcgagcgcc agcgcactgt caccgtctcg tgcgagctgg ctgagggcgt
     1081 tgccttgggg aatgcccagt tggcgatcaa cgagcggttg gccaaacttc agacaaacgg
     1141 cgtctccgtt cgctgggtcg ggcaggccga gttcatggag gagtcgaacc gcgacatgat
     1201 gagcgccctg gggctctcgg tgctcctggt gttcatgctc atggccgcgc tgttcgaatc
     1261 gctcctgtcg ccgctcatca tcatgctctc cgtgcctcag gcaatggcgg gcgccttctt
     1321 cgcgctcacg ttcacgggga aatcgctgag catgccctcg atgatcggta tgatcatgct
     1381 ggtcggcctt gtcacgaaga acgccattct gctggtcgac tacaccaaca ccctgcgtaa
     1441 cgagcacggg atggagcggc gcgcggcact gctgcaggcg ggcccgaccc ggttgcagcc
     1501 gatcctgatg acaacactgg cgatgatcgg cggcatgctg ccgacggcga tggcgctcac
     1561 gaagggatcg gagatgcggc agccaatggc catcgctgtg atcggcggcc tggcactttc
     1621 gacgttcctt acgctgctga tggtgccggt gttctatgag gtgatggatg acctgggcga
     1681 gcggtttgcg cgcgcgaagg aagcggttat ccgcctgctg agggtgtaac ccgcccaagg
     1741 cggtggcttg catgcgtgct gatcgctgct ggctggtcat gcccgggagg acggcgagag
     1801 cgccggtcct cccgggcgct agaagggggt gggaggtgtg agagggttgg atgctgtgac
     1861 acataaggcg acacgaccgc gtgtacgcct gcttccggaa gagcgccggc ggcaacttgt
     1921 ggacgtcgca ctccgcctct tcgcggagaa tggctatgac gccacgac
//
LOCUS       DAOSCV010000876         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38392_length_1965_cov_8.215707, whole genome shotgun sequence.
ACCESSION   DAOSCV010000876 DAOSCV010000000
VERSION     DAOSCV010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38392_length_1965_cov_8.215707"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1293
                     /locus_tag="PLY56_17250"
     CDS             505..1293
                     /locus_tag="PLY56_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23277.1"
                     /translation="MFGTLFRVLLLVGLGIVVAGAALLALKAVVALTVFVITLLLPFL
                     VVAWLLRYALGERLRVPSPPIPARERGWRWGPRVKPKEEDPEEWSREASALAAEIAHL
                     VRNGNRGTRQLLSSAPGGARKLAKRVRELSRLWHTLDDHLRTNEATEFLRRAEAMRAR
                     ADAASDRFVQEQYAEAARSLESQAQTCLELGHTRERLRAEIARILAALTNLRSRIVAV
                     QASPADGSDAVRRAAEELADLEAQVGTFQESVRQVLRQASQASR"
     gene            complement(1290..>1965)
                     /locus_tag="PLY56_17255"
     CDS             complement(1290..>1965)
                     /locus_tag="PLY56_17255"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOJ23278.1"
                     /translation="SRVCVEVVDPDQGVHAKFNEPGPHVTAMDAVRLESKISGWLERA
                     RLVVLSGGGPPGTPSDFYARLICRIRAAGVRVLLDTSGELLRRGVAAAPYLVKPNAEE
                     LADLMGTPVTSPEEAARGARALLQSGVAVAAVSLGEQGAVLAHGTDLLYAEPPSTDVV
                     STVGSGDCMVAGFAAALVRGMETEAMLRLGVAAGTANAAVLGPGLCTRAEIERLAPQV
                     KIASLS"
BASE COUNT          320 a          686 c          639 g          320 t
ORIGIN      
        1 gcgggccgga ccaataggcg cgcatgcctg aaaactcgcg ctgctaccca gcacccgggc
       61 cgtaaacgca tcacggccgg ttgttggggg accacccagc gcattgcggt ggccgatctc
      121 gcgagcaccc atcgtaggcg ggaacccgtg gcgatcagcc agtagattgc agttccagcc
      181 acatcgctgg gattcggaca taaggagaag accgcgcgca cgcgcggagc tcgaagctcc
      241 gcgctgcttt ccggcaaagt tccttcgggg ctccccctcc cagccaccgc cgttttgaga
      301 catctgggag tatccccaac ccagcccggc agggcttcgc tcgcaagcag cgcggtgatt
      361 tatcgccgcg cgccgggttg cgccctgcct ccgaggcttc gaactcctta tgtcggaatc
      421 tcaggccgca tcgggcgcgg tttgacccgg tgttacgccg gatggtataa tgatcaaggc
      481 acggttcttg aaaggcaagg ggccatgttc ggaacgctgt tccgagttct cttactcgtt
      541 ggactgggca tcgtagtggc aggcgcggcg ctactggcgc tgaaggcggt ggtggcgttg
      601 acagtcttcg tgattacgct gctgctgcca ttcttggtcg ttgcctggtt gcttcgctac
      661 gccctgggag agcggcttcg cgtcccctcg ccgcccatcc ccgcgaggga gcgaggctgg
      721 cgctgggggc cacgcgtcaa gcccaaggaa gaggatccgg aggagtggtc gcgagaggcc
      781 tcggcgctcg cagctgagat cgctcacctg gtgcgcaatg gcaatcgtgg cacccggcag
      841 cttctttcga gcgctccggg gggggcgcgc aagttggcca agagagtgcg cgagctctct
      901 cggctctggc acaccctcga cgaccaccta cgtaccaacg aggccacgga gttcctccgc
      961 cgggcggaag cgatgcgcgc gcgcgccgat gcagccagcg accgcttcgt tcaggagcaa
     1021 tacgccgaag cggcgcgctc gctcgagtcg caggcgcaga cttgtctgga actggggcat
     1081 acccgcgagc gcctgcgcgc cgagattgcc cggatccttg cggcgctcac caacctccgt
     1141 tcgcgcatcg tcgccgtcca ggccagtccg gccgacggct ccgacgccgt ccggcgcgcc
     1201 gctgaagagc tcgccgatct ggaggcacag gtaggcacgt tccaggagag cgtccgccag
     1261 gtgctgcgcc aggcttccca agcctcgcgc taactgagcg aggcgatttt gacttgcggc
     1321 gccagtcgct cgatttcggc acgggtgcag agccccggtc ccaacaccgc tgcgttggcg
     1381 gtgcctgccg ctacacccag gcgtaacatg gcctccgtct ccatgccgcg cacgagtgcc
     1441 gcggcaaagc cggccaccat gcagtcgccc gatccaaccg tgctcaccac atccgtggat
     1501 gggggttccg catacagcag gtccgtgcca tgggccaaca ccgcgccttg ctccccgagc
     1561 gatacagccg cgaccgccac gccgctctgt agcagggccc gcgcgccccg ggctgcctct
     1621 tccggcgagg tgaccggcgt gcccatgaga tcggccagtt cctcggcgtt cggcttcacc
     1681 agatagggcg ccgccgccac cccgcggcgc agcaactcgc ctgaggtgtc gagcaacacc
     1741 cgaacgcccg ccgcgcggat ccgacagatc aggcgcgcgt agaaatccga gggggtcccc
     1801 ggcggtccgc ccccagagag gacgacgagc cgggcacgct cgagccaccc ggagatcttc
     1861 gactccaggc gcaccgcgtc catcgccgtc acatgcggtc cgggctcgtt gaacttggcg
     1921 tgaactccct gatctgggtc gaccacctcc acgcagacgc gcgat
//
LOCUS       DAOSCV010000877         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38411_length_1965_cov_2.691623, whole genome shotgun sequence.
ACCESSION   DAOSCV010000877 DAOSCV010000000
VERSION     DAOSCV010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38411_length_1965_cov_2.691623"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1965)
                     /locus_tag="PLY56_17260"
     CDS             complement(<1..>1965)
                     /locus_tag="PLY56_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23279.1"
                     /translation="QSRPVGRGIEFSGSVDDPRGTDGTDHAVTLRFALPVKLEGWRMD
                     RDLDRSWPLRGDSHRSPASPCPWGRGRGELWPVVAVACEKASLGLGCRLDEPRCFRVT
                     YDGPAQLLSIEIDFALARSAKQPGKAGFRFLILARPDSWGLRGCLADYYATYPELFRK
                     RTELAGGWFAWGDILSLAAPAVDFGLAFHEGPENAKARIHSLALGAATFPYIEPLMYQ
                     LPMGDFDRAPVRAEIEERIRLWSKPLDPLPKMHRGHYDQTRCAATLASGIRDPDGSLH
                     IAAISQYPWIAGTRWAAQLALNVDPEIPGGAGELYLAEERENIKSETWGEGRYLDSFS
                     SHANKIDYAPDHLAHADHPLIWKADEQAPGGFRVGQPVAAAAFEYAQGLRELLDTRGK
                     LILVNQYGHGAPAPFHVFDCLGKEYWVPGAGRLLQRYRATSYQKVVSNLPRNEPISDR
                     QLREFLVYGIFPGGYGRPGWGEEGMRATYRQVVPLLRLQHRLGWEPVPHAVAKESGVL
                     IERFGGTGGKPLCLAVHSRWGAKVVTLTLDHAALGLPGALWCHELVSDDPVTWVTEAG
                     QTEIQLGLASGETRLLAVGDARTHAAIARILAEDRLRDARLCTAEYRLREGRAHLLER
                     HLQTASHENASALLSLAERVTGGHALDQRIA"
BASE COUNT          311 a          666 c          689 g          299 t
ORIGIN      
        1 gcgatgcgct ggtcgagggc gtgcccgccg gtgactctct ccgccagcga gagcaatgcc
       61 gacgcgttct cgtgcgacgc ggtctggaga tggcgctcaa gcaggtgcgc cctcccttca
      121 cgtagccggt actccgcggt gcagaggcgc gcgtcgcgca gccggtcttc ggccaggatt
      181 cgggcaatcg ccgcgtgcgt gcgggcgtcg ccgacggcga ggaggcgcgt ctcgccagag
      241 gccagcccga gctgtatctc tgtctgtccg gcttccgtga cccaggtgac cgggtcatcg
      301 ctcaccagct cgtggcacca gagggcgcct ggaagcccga gcgcggcgtg gtccagggtg
      361 agcgtcacga ctttcgcgcc ccatcggcta tgcacggcca ggcagagtgg cttgcccccg
      421 gtgccgccaa accgctcgat gaggacgccg ctctccttcg cgacggcgtg cggcacgggc
      481 tcccagccga gccggtgctg caggcggagg agtggcacga cctgccggta ggtcgcgcgc
      541 atcccctctt cgccccatcc tggccgaccg tagccgcccg ggaagatgcc gtagacgagg
      601 aactcgcgga gctgcctgtc agaaatcggc tcgttgcgcg gcaggttact taccaccttt
      661 tgatagctcg tggcgcggta gcgctgcagg aggcgcccgg cgccaggcac ccaatactcc
      721 ttgccgaggc agtcaaagac gtggaacggc gcgggcgcgc catgcccgta ttggttgacg
      781 aggatcagct tgccgcgcgt atccaggagc tcacgcaagc cttgggcata ctcgaacgca
      841 gcggcggcga ccggctgccc cacccggaac ccgcccggtg cctgctcgtc cgccttccag
      901 atcagggggt gatccgcgtg ggccaggtgg tccggcgcgt aatcgatctt gttggcatga
      961 gaggagaagc tatcgagata gcggccctcg ccccacgtct cgctcttgat attctctcgc
     1021 tcctcggcca ggtagagctc gcctgcgccc ccagggatct ccgggtcgac gttgagcgcg
     1081 agttgggcgg cccagcgcgt gcccgcgatc cacgggtact ggctaatcgc cgcgatgtgg
     1141 aggctgccgt cggggtcgcg gatgccgctc gccagcgtag cggcacagcg ggtctggtcg
     1201 tagtgaccgc ggtgcatctt cggcagggga tcgaggggct tcgaccagag ccggatccgc
     1261 tcctcgatct ccgcgcgaac gggcgcacgg tcgaaatcgc ccatcgggag ctggtacatc
     1321 aacggctcga tataggggaa ggtggccgcg ccgagcgcca gcgagtggat gcgcgccttc
     1381 gcattctcag ggccttcatg gaaggctagg ccgaaatcaa ccgctggcgc ggcaagcgag
     1441 aggatgtcgc cccaggcaaa ccagccccca gccagctccg tgcgtttgcg gaacagctcg
     1501 ggataggtgg cgtagtagtc ggcgaggcac ccgcgcaagc cccaggaatc cggacgcgcg
     1561 aggatgagga agcggaaacc ggcctttccg ggctgcttcg ccgacctggc gagcgcgaag
     1621 tcgatctcga tggagaggag ctgcgccgga ccatcatacg tgacacggaa gcaccggggc
     1681 tcgtccaggc ggcagccaag gcccagtgac gccttctcgc acgccacggc cacgaccggc
     1741 cagagttccc cgcggccccg gccccagggg cagggagacg ccggcgagcg gtggctgtcc
     1801 ccgcggagtg gccacgagcg atccagatcg cggtccatcc gccagccctc cagcttcacc
     1861 gggagggcga agcggagcgt cacggcgtgg tctgtcccat cggtgccacg cgggtcgtcc
     1921 acgctcccgg aaaactcgat cccgcggcct accgggcgcg actgg
//
LOCUS       DAOSCV010000878         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38448_length_1964_cov_2.689366, whole genome shotgun sequence.
ACCESSION   DAOSCV010000878 DAOSCV010000000
VERSION     DAOSCV010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38448_length_1964_cov_2.689366"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1964
                     /locus_tag="PLY56_17265"
     CDS             <1..>1964
                     /locus_tag="PLY56_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23280.1"
                     /translation="VQSVTEEPKRVLFRGVADLYEAFLERAQADNPEWVVLKTAPGVD
                     VVGARNNRGIQVLVVFYREEVGERESLNAFRVFNEADVASALVARAADGGLGVTFSPG
                     ARDRVQSILRLVYPSAEDLANFLLRGESQMGDALPFVGLFRDPDLPVTLALPRWQARV
                     QENYEAAVLRWREFLEKGVRSQASQRLLGNRVALLRQAAEEPSARAPVLTQVSLAEAL
                     RVLNPPNRVVGRLLQLPEWTHDRAEAFYADVRAGRVDPYEPPAGTPPLPRDVVDLLDR
                     IRPKTDDDENTVESGEAAAPRLRFCLQALLTLAAQDDSLPQRLRLTRPESEDQEFADL
                     VRDPDTGRWSVAFSDEGARRLSTLASGASELAFALYRPGESERAVLRFSLDYLAGRLT
                     SHEECWPDEEAWNRAEGIDPAHAPAWRALRQATARLQEIVDPEWRTAQDERESNNAIY
                     QVFDLLYLAHRDAFEEFLQAWVTVARVPWRNLRPDHRLAWRHSVQEVLLRLGLAKGDG
                     SLAVLPYHPLRLAWHRTIFSQIEGWLTQRVESGRPLVFDDDVLSQQCECIDRPRALFD
                     PCATDADERSTRWMEASRAPFFSLFVPEKEHRRPRAPLDRADQKMQQFGRMWPFSLSR
                     LHIAFQPGDAAGDVYRVVANRAEHEPETAF"
BASE COUNT          304 a          671 c          660 g          329 t
ORIGIN      
        1 tgtgcagtcg gtgaccgagg agccgaagcg ggtcctcttt cggggggtcg cagacctcta
       61 cgaagcgttc ctggagcgcg cgcaagccga taacccggag tgggtggtgc tgaagacggc
      121 cccgggtgta gatgtcgtcg gagcgcggaa caaccggggc atccaggttc tggtggtctt
      181 ctaccgcgag gaagtgggag agcgggagtc actcaacgcg tttcgcgtgt tcaacgaggc
      241 ggatgtggca tcggcgctcg tcgcccgggc ggctgatggc gggcttggag tcaccttctc
      301 gccaggggcc cgcgaccggg tccagagcat cctccgactc gtctacccct ctgcggagga
      361 tctggcgaac ttcctactca ggggggagag ccagatgggg gatgccctcc cgttcgtcgg
      421 tctcttccga gaccccgacc tgccggtgac cctggccctg ccccgatggc aggcgcgcgt
      481 ccaggagaac tacgaagccg ccgtcctccg ctggcgcgag ttcctggaga agggtgttcg
      541 cagccaggca agccagcgtc tcctcgggaa tcgggtggcg ttgctccggc aggccgcgga
      601 ggagccctcc gctcgtgctc ccgttctgac gcaggtgagc ctcgccgagg ccctgcgcgt
      661 gctcaacccc cccaaccgtg tggtgggccg ccttctgcag ctgcccgagt ggacccacga
      721 ccgggccgag gcattctacg ccgacgtgcg cgcggggcgc gtggacccct acgagccacc
      781 cgccggaacc ccgcctctac ctcgcgacgt ggtcgacctg ctggaccgca tccgaccaaa
      841 gaccgatgat gatgagaaca ccgtggagag cggcgaggcc gcggctcccc ggctgcgctt
      901 ctgccttcag gcactcctga cgctggcagc acaggacgac tcgctaccac agcgcctccg
      961 ccttacccgc ccggagtccg aggatcagga gttcgccgac ctggtccgcg acccggacac
     1021 ggggcgttgg agtgtggcgt tcagcgatga gggcgctcgg cgcctgagca ctctcgcatc
     1081 tggcgcttcg gagctcgcct tcgcgctcta tcggccgggg gagagtgaga gggctgttct
     1141 gcgcttctcg ctcgactacc tggccggacg cctcacctct cacgaggagt gctggccgga
     1201 cgaggaagcc tggaaccggg cagagggaat cgatcccgcg cacgccccgg cgtggcgagc
     1261 cctacgccag gcgacggccc ggctacagga gatcgtcgac cccgagtggc gaaccgcgca
     1321 agatgagcgc gagtcgaaca acgccatcta ccaggtcttc gatcttctct atctggccca
     1381 ccgggacgcc ttcgaggagt tcctccaggc atgggtcacc gtggcacgcg ttccgtggcg
     1441 caacctgcgg ccggaccacc ggctggcatg gcggcactcc gtccaggagg tccttctccg
     1501 ccttggactg gcgaagggag acggctccct tgccgtgctc ccctaccatc cgcttcgtct
     1561 ggcctggcac cgtaccatct tctcccagat cgaagggtgg cttacccagc gtgttgagtc
     1621 cgggcgcccg ctggtgtttg atgacgacgt gctctctcag caatgcgagt gcattgaccg
     1681 gccgcgggcg ctgttcgatc cttgcgcaac agacgccgac gagcgtagca cgcgttggat
     1741 ggaggcatcg agggcgccct tcttcagtct cttcgtgccc gagaaggagc accgccggcc
     1801 gcgcgctccc ctggaccggg ccgaccagaa gatgcagcag ttcgggcgca tgtggccctt
     1861 cagcctctcc cggctccaca tagcctttca gccgggcgac gcagcgggcg atgtctaccg
     1921 ggtcgtggcc aaccgggctg agcacgaacc agaaacggcc ttcc
//
LOCUS       DAOSCV010000879         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38499_length_1962_cov_3.487153, whole genome shotgun sequence.
ACCESSION   DAOSCV010000879 DAOSCV010000000
VERSION     DAOSCV010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38499_length_1962_cov_3.487153"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PLY56_17270"
     CDS             complement(<1..1199)
                     /locus_tag="PLY56_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ23281.1"
                     /translation="MDEQSLRVLEYERVRELLLAHASSELGKERVRAMVPLTDLREIE
                     QRQAETAEACLAIEEQGGIPLGGIRDIRVAVDRAARDGILQPQELLDIADTVYSARRL
                     KFFFARHRSRFPLLNEVATHIYDFSALEHEIRAAISDRAEVQDNASPELAHIRSSILI
                     THSRVHERIEQILHSPAYEKMIQEPVVVRRSDRLCIPIKAEYRHAFDGLVHDTSASGA
                     TVFMEPAAVLALQNDLRELEANEKHEVERILTALSHLVRRRAQELHATLHALGIIDFI
                     HAKAQLSRAQQAVRPRLNSQGRIDLRGARHPLLQGNVVPIDVHLGKEFTTLVITGPNT
                     GGKTVALKTLGLLTLMAQSGLHVPAADGSEMAVFKQVFADIGDEQSIEQSLSTFSAQI
                     RQIVKIVR"
     gene            1665..>1962
                     /locus_tag="PLY56_17275"
     CDS             1665..>1962
                     /locus_tag="PLY56_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23282.1"
                     /translation="MTPAGDAVQLGMAERLHYERLIVKLRWVVIAWILSLALFGDQAG
                     ARGGAEYTALVVILICSAYFTAAVYRREKPVCFGRRGFMIDFLSEPIQPPMQSMS"
BASE COUNT          321 a          634 c          642 g          365 t
ORIGIN      
        1 cggacgatct tgacgatctg ccggatttgc gccgagaagg tggaaagcga ctgctcgatg
       61 ctctgctcgt caccaatatc ggcgaaaacc tgcttgaaga cggccatctc cgagccgtcc
      121 gccgccggaa catgcagccc cgattgcgcc atcagcgtga ggagcccgag cgtcttcagt
      181 gccaccgtct tcccgcccgt gttcggaccg gtgatcacca gcgtcgtgaa ctctttcccc
      241 aggtggacat cgatcggcac cacgttgccc tgaagcaggg ggtggcgcgc gccacggagg
      301 tcgatcctgc cctgggagtt gagccgtggg cgcaccgcct gctgcgcgcg gctgagctgc
      361 gccttcgcgt ggatgaagtc gatgatgccg agcgcgtgca gcgtggcgtg cagctcctgt
      421 gcgcgccggc gcactaggtg tgagagcgcc gtcagaatcc gctccacctc gtgcttctcg
      481 ttcgcctcca gctcgcgcag gtcgttctgg agcgccagca ccgcggccgg ctccatgaaa
      541 accgtggcgc cgctcgcgga ggtgtcgtgg accagtccat cgaaggcgtg gcggtattcc
      601 gccttgatcg ggatgcagag gcggtcgctg cgccgaacaa ccaccggctc ctggatcatc
      661 ttctcatagg caggcgagtg gaggatctgc tcgatgcgct cgtgcacgcg agagtgcgtg
      721 atgagaatgc tgctgcgaat gtgtgccagc tccggactgg cgttgtcctg cacctcggcg
      781 cggtcactaa tggcggcgcg gatttcatgc tcgagggcgc tgaagtcgta gatatgggtg
      841 gccacttcgt tcagcagcgg gaagcggctg cggtggcgcg cgaaaaagaa cttcagccgc
      901 ctggcggaat agaccgtgtc ggcaatatcc agcagctcct gcggctgcaa gatgccgtca
      961 cgcgcggcgc gatccaccgc gacgcggata tcgcgaatgc cccccagggg gatgcccccc
     1021 tgctcctcaa tcgcgagaca cgcctcggcc gtctccgcct ggcgctgctc gatctcacga
     1081 agatcggtca gtggcaccat agcgcggacg cgttccttgc cgagctcgct cgacgcgtgc
     1141 gccaacagca gttctcgtac tctctcgtac tccagaaccc tcagtgattg ctcgtccata
     1201 gttggtctca ttatagcacg cggtcccccc tgggtcaaac ttcgccgagg cgcgagaaac
     1261 cgcatggttg cttgattcct ggcccccaag ccggcgcttg gcgtgccgtg cgcgggcacg
     1321 cgagcgcccc ctggagagga tgaagagaga cgcgcctccg ggctagcgtg tggcatcgcg
     1381 gccaatggca cactccgtgc acttccgcgc catgccacac cagaaacggg cccgcccgct
     1441 tcaccggtgt gctggatcgg gcgagtaggg gaggggactg tctgatcccg gcaccatgcc
     1501 aggatgctgg ctccgcgagt ggcgatttcg cacgcgcggc gagcagcttg atctcgcttc
     1561 ctgcacttta cccgcgcgcc ggcacaggca gccattgaac caagcccgtg cgtggggccc
     1621 ggcatgcccc tttgccacgc agtggcctat cgcgaggagg tgcaatgacg cctgcgggtg
     1681 acgccgtgca acttggcatg gcagagcggt tgcattacga gcgactcatc gtgaagctgc
     1741 ggtgggtggt catcgcctgg attctctccc tggccctgtt tggcgatcag gcaggggcgc
     1801 gcggaggggc cgaatacacg gcgctcgtgg tgatcctgat ctgcagcgcc tacttcacgg
     1861 cggcggtcta tcgccgggaa aagccggtgt gttttggccg gcgcggcttc atgatcgact
     1921 ttctcagcga acccattcag cccccaatgc agagcatgtc cg
//
LOCUS       DAOSCV010000880         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38538_length_1961_cov_3.403463, whole genome shotgun sequence.
ACCESSION   DAOSCV010000880 DAOSCV010000000
VERSION     DAOSCV010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38538_length_1961_cov_3.403463"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..690)
                     /locus_tag="PLY56_17280"
                     /pseudo
     CDS             complement(238..690)
                     /locus_tag="PLY56_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(717..1076)
                     /gene="ndhC"
                     /locus_tag="PLY56_17285"
     CDS             complement(717..1076)
                     /gene="ndhC"
                     /locus_tag="PLY56_17285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOJ23283.1"
                     /translation="MLASYFPILVMLVMAIGFAVTMILLSSFTGRKRPSAAKESVYEC
                     GSLPVGSARLRFDVKFYVVAILFILFDLEVVFLWPWAVLHAGQLKLAGLGTVALFVAI
                     LLAGYLYAWRKGALDWE"
     gene            complement(1322..>1961)
                     /locus_tag="PLY56_17290"
     CDS             complement(1322..>1961)
                     /locus_tag="PLY56_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23284.1"
                     /translation="PVPEGTVPEEAPAKVSVPIRYEHRLAGTLPVYGDLRALFPMQPD
                     VIEITLITGGKSFTGWLNNANNLALEFGPFYDRLDLPLCGGCFQLQPRGRGITTDFTV
                     SYTPGDVDELVAISDERLAVLESMREDPENVQTSTFDLLRQIMEPYGKKGVHFVTLFT
                     EVNVVRRTHAYLIASLLSAYACFSHVKPGTWAYDEKKVDQGIRKQKRKFIKE"
BASE COUNT          379 a          670 c          585 g          327 t
ORIGIN      
        1 ctctcgcgca ccttctcgac tgcgacacac ttctctgcct gagtctcgct cacatccatc
       61 actccctatg ggcccgcgcc cggagacgtc tcgccggagg aatacacctc cgcgctggca
      121 cgccatccct ctccgcgaga aaggctgctc gccacggcag ggtggcggcc gtcgccagag
      181 actcctgtct cacccgccct tcgccggcaa ttatcggcgg agtggccggt cctgccggat
      241 ctgctcgtgg atctgcagga tcgccgagat cagcgcctcg gggcgtggcg ggcaccccgg
      301 caggtagata tcaaccggga tgatctgatc gacaccctgc acaacggcgt agttgttgaa
      361 gacattgccc gtcgaagcgc acgagcccat cgcgatcacc cacttcggtt ccgccatctg
      421 gtcgtagatc gcccggaccg ccggggccat cttccggctg aggcgcccgg aaacaatcat
      481 caggtcggcc tgccggggcg aggcacgaaa cacctctgcg ccaaagcgcg agagatcggc
      541 gcgggcgccc acggtcgcca tcatctcgat agcacaacac gccaggccaa aggtgagcgg
      601 ccagatggag gacttgcgcg cccagttgac catcgcctcg acggtcgtca caaagacgtt
      661 ctccttgacc tgctcttcca gcgggctcat cgccccccct tcccgggcca ccggcctcac
      721 tcccaatcga gcgccccttt ccgccaggca tagaggtagc cggccaggag gatcgcgaca
      781 aagagcgcca ccgtaccaag gccggccagc ttcagctgcc cggcgtgcaa cacggcccac
      841 ggccagagga agaccacttc caggtcaaaa aggatgaaga ggatagcgac cacgtagaac
      901 ttgacgtcga accggaggcg cgcgctgccc actggaagag agccgcactc atacaccgac
      961 tcctttgccg ccgagggccg cttcctcccc gtgaacgagg agagcaggat catggtcacc
     1021 gcgaatccga tggccatcac cagcatcacc agaatgggaa aatagcttgc cagcacgcgc
     1081 tcgctcctcc caaccgccct ggaagggacc aagggggccc aggcgctgcc cgatgccaag
     1141 tcggacatcc ctggttcccc cttgacttta cggaacccga acccgccgcg ccctcccgct
     1201 cggccttcca ccagcgcgac gcgctcgtga tgcgcacaga acgcatcccc aattaggaga
     1261 acaacggaac gcttgctctg gttccctgag gggcaggcgc tccgttgtta gtttacccag
     1321 ctcactcttt gataaacttc cgcttctgct tgcggatgcc ttgatcgacc ttcttttcgt
     1381 cgtaggccca cgtgccgggc ttcacgtggg agaagcaggc gtaggccgaa agcagggaag
     1441 caatcaggta ggcgtgggtg cgccgaacga cgttcacctc ggtgaagagc gtcacgaagt
     1501 gaacaccctt cttgccatag ggctccatga tctggcgcag cagatcgaag gtggaggtct
     1561 gaacgttctc ggggtcctcg cgcatcgact ccagcaccgc gaggcgctcg tcgctgatcg
     1621 caaccagctc atccacgtcg ccgggcgtgt aggagacggt gaagtcggtg gtgatcccac
     1681 gtccgcgtgg ctgcagctgg aagcaaccgc cgcacagcgg caggtcgagg cgatcataga
     1741 aggggccaaa ctcgagggcc aggttgttgg cgttgttcag ccatccggtg aagctcttgc
     1801 ccccggtgat cagcgtgatc tcgatcacat ccggctgcat cgggaagagc gcccgcaggt
     1861 cgccataaac gggaagcgtg cctgccaggc ggtgctcgta gcggatcggc acggagacct
     1921 ttgccggcgc ctcttccggc accgttccct caggaaccgg c
//
LOCUS       DAOSCV010000881         1959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38589_length_1959_cov_3.578256, whole genome shotgun sequence.
ACCESSION   DAOSCV010000881 DAOSCV010000000
VERSION     DAOSCV010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38589_length_1959_cov_3.578256"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLY56_17295"
     CDS             complement(<1..313)
                     /locus_tag="PLY56_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365095.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     from 3-phospho-D-glycerate in serine biosynthesis; can
                     also reduce alpha ketoglutarate to form
                     2-hydroxyglutarate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ23285.1"
                     /translation="MRVLVSDPVAAEGVEILRGGADVDVITGLTREELIARIKEYDGL
                     VVRSETKVTAEVIQAAERLKVIARAGVGVDNIDVPAATHRGIVVVNSPEGNTIAAAEQ
                     AL"
     gene            complement(423..1571)
                     /locus_tag="PLY56_17300"
     CDS             complement(423..1571)
                     /locus_tag="PLY56_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOJ23286.1"
                     /translation="MRNKQILAIPGPTPVPPEARLAMARPMINHRGSEYTAMQREIVE
                     GLQYAFQTGGDVLIFPASGTGGLEAAVVNTLSPGDRVLSGSIGAFGDRFADIAEAHGA
                     IVDRIPTEWGSALDPEVLRARLAEDRDHTIKAVLLTHNETSTGVTNDIRALAEVIRDH
                     GALVLVDSISGLLAADLQTDAWGLDVVVAGSQKAFMIPPGMTFVSVSERAWAAHRNAR
                     MPRYYFDFTAMKKYMEKGQTPYTPAVSLLYGLQTTLRMLREEGLPECFARHARLGAAV
                     RAGAQALGLRLLADPRYASPAVTSIYAPEGIDPKQLRQVVRERYGVVLAGGQGKLADR
                     IFRIGHLGYVGENDIIAVMSALERGLGDLGVRITPGAGIAATQQALAA"
     gene            complement(1766..>1959)
                     /locus_tag="PLY56_17305"
     CDS             complement(1766..>1959)
                     /locus_tag="PLY56_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23287.1"
                     /translation="LDLTVHQAGVYRVEVLRPSGRLGAFCLTPRPWIYSNPIYVRPGH
                     AALARLQAREQAAGSRIRM"
BASE COUNT          325 a          619 c          683 g          332 t
ORIGIN      
        1 ccaacgcctg ctccgcggcg gcgatggtgt tcccctcggg cgagttgacc acgacgatcc
       61 cgcgatgggt ggcggcaggc acgtcgatgt tgtccacacc cacgccggcg cgcgcgatca
      121 ccttgaggcg ctcggcggcc tggatcactt ccgccgtcac cttcgtctcg ctgcgcacca
      181 ccagaccatc gtactccttg atgcgggcga tcagctcttc cctagtgagg ccggtgataa
      241 cgtcgacatc ggcgccgccg cggaggatct ccacgccttc ggcagccacc ggatcggata
      301 ctagtactcg catggtttct cctgagttga tccgctggat tcgccggcgc gctccagaag
      361 aggccgtcga atgccctggc ggcgtgaggg ccgcctgggt ttgcaccggc gaatggcgcg
      421 ggttaggcgg ccagcgcctg ctgggtggcg gcaatgccgg ccccgggcgt gatccggact
      481 ccgaggtcgc cgaggccgcg ttcgagggcg ctcatcacgg cgataatgtc gttctctccc
      541 acgtagccga gatggccaat gcggaagatg cggtcagcca gcttgccctg gccaccggcg
      601 aggaccacgc cgtaccgctc gcggaccacc tggcgcagct gcttcggatc gatcccctcg
      661 ggcgcataga tggaagtgac cgctggactg gcatagcgcg ggtctgccag caggcgcaat
      721 ccgagggctt gcgcgccagc gcgcacggcg gcgccgagcc tggcatggcg ggcgaagcac
      781 tccggcaggc cctcctcgcg gagcatcctc agcgtggttt gcagcccata gagaagcgag
      841 acggccggcg tgtagggcgt ctggcccttc tccatgtact tcttcatcgc cgtgaagtcg
      901 aagtagtagc ggggcatgcg agcattccga tgggccgccc aggcacgctc gctcaccgag
      961 acaaaggtca tgcccggcgg gatcatgaac gccttctgcg agccggcgac caccacatcc
     1021 aggccccacg catctgtctg aaggtccgcc gcgagcaggc cactgatgga gtcgaccagg
     1081 acgagggcgc catgatcacg gattacctcc gcgagcgcgc ggatgtcgtt ggtgacgccg
     1141 gtggaggtct cgttgtgcgt caggagcacg gccttgatgg tgtggtcgcg gtcctcggcc
     1201 aggcgggcgc gaagcacctc aggatcgagg gcgctgcccc actcggtggg aatgcgatcc
     1261 acgatggcac catgcgcctc agcgatatcg gcaaagcggt cgccaaaggc accgatgctc
     1321 cccgaaagga cgcggtcgcc cggcgagagg gtgttgacga cagcggcttc gaggccgccg
     1381 gtaccagaag cggggaagat gagcacgtca ccgccggttt gaaaggcata ttggaggcct
     1441 tccacgattt cgcgctgcat cgcggtgtat tccgagcccc ggtggttgat cataggacgg
     1501 gccatggcca agcgcgcctc aggcggaaca ggtgttggcc cggggatggc gagaatctgc
     1561 ttgttgcgca ccttcaggtc tcctcaaagt atcaaggcag cagtatggaa gggctgctcc
     1621 tcccacgtca gatcatacac gatgggtggg gtgatgtcaa ccgaagtggt gcattccacg
     1681 aggccatttc gtccagcgcg cggtgcgtgt gccctctcgg ccgggccggc cttgtcggtg
     1741 ccggcttggc gagcgcgcag ccagattaca tgcggatgcg ggatcctgcc gcctgctctc
     1801 gtgcctgaag gcgtgccagg gcggcatgcc cggggcgcac gtaaatcggg ttgctgtaga
     1861 tccagggacg gggggtaagg cagaaggctc ccaggcggcc gctggggcgg agcacttcga
     1921 cgcggtagac gccggcctgg tggaccgtga gatcgaggt
//
LOCUS       DAOSCV010000882         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38610_length_1958_cov_3.716763, whole genome shotgun sequence.
ACCESSION   DAOSCV010000882 DAOSCV010000000
VERSION     DAOSCV010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38610_length_1958_cov_3.716763"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PLY56_17310"
     CDS             complement(<1..214)
                     /locus_tag="PLY56_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23288.1"
                     /translation="MTYETTEISNGLPVIFSPAPGTPRTCICITMRGGSRTQEKPGMA
                     GLAARLLLKGTSSRSAEQIADEIDRRA"
     gene            complement(211..1548)
                     /locus_tag="PLY56_17315"
     CDS             complement(211..1548)
                     /locus_tag="PLY56_17315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ23289.1"
                     /translation="MKEGIREVVLPYLARPAGVATLDSGHTVVVIPKPGDVVHLHTVV
                     RVGSALEDDETNGISHFLEHLMFKGTPRYPAGDFDRILEGMGARINASTSKDWTQYYA
                     TLPKGEEGRFYRRALELHADMLLNPLLPENEIGPPFDPANPEVEQKRERHVVIEEIKM
                     GKDNPWRRCLQALNSLMYRHHPYKREVIGTEEVIARVSRETLMGYYRRWYAPDNMVTI
                     VAGDLEIEPTVEEVRQEFQFGDQRVGGLPVFPPEPEQTEMRVEELYGDVQTGYVLAGF
                     RGAQDSLEETIALDIAMVVLGEGKSSRLHQRLIETLADSPFFDLGAAQWEYRDETSLV
                     TYGVCRADSVARSVDLLKEQIAGLASDPPSVSEFEKARTRLEARFAAEAEWAMALTLR
                     VADSMVRRGDLSAYIDYLPTLQSLTPADVSAAIQRYMQADRLCLVTMSPGSDR"
     gene            complement(1754..>1958)
                     /locus_tag="PLY56_17320"
     CDS             complement(1754..>1958)
                     /locus_tag="PLY56_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23290.1"
                     /translation="RGSRLLWSWRRARSRRSIWASSFAGKPGAGVYIHVIPGPRYVIP
                     ALHLRMQGRETIRGLACQPARMV"
BASE COUNT          339 a          673 c          605 g          341 t
ORIGIN      
        1 tcgcgcgccg gtcaatctca tcggcaatct gctctgccga gcgcgaagag gttcccttga
       61 gcaagaggcg cgcggcgagg cccgccatgc ccggcttctc ctgggtgcgg gagcctccgc
      121 gcatcgtaat gcagatgcac gtccggggcg tgccaggcgc cgggctgaag atcacaggca
      181 gaccattgga gatttccgtc gtctcgtacg tcatcgatca ctcccagggg acatcgtcac
      241 gaggcagagg cgatccgcct gcatatatcg ttggatcgcc gccgacacat cagccggcgt
      301 caggctctga agggtcggaa gatagtcaat ataggcggag aggtcgcccc ggcgcaccat
      361 cgaatcggcc acgcgcaaag tgagagccat cgcccactcg gcctcggcgg cgaagcgcgc
      421 ctccaggcgc gtccttgcct tctcaaactc cgaaaccgat ggcggatccg atgccaggcc
      481 cgcgatctgc tccttcagca ggtcaacgct gcgagccacc gagtctgcgc ggcagacgcc
      541 gtaggtcacc agcgaagtct cgtctcggta ctcccactgc gccgcgccca ggtcaaagaa
      601 gggcgaatcg gccagcgtct cgatgaggcg ttgatgcagc cggctgctct tcccctcgcc
      661 caggacgacc atggcgatat cgagcgcgat cgtctcctcc agcgagtctt gggcgccacg
      721 gaagccggcc aggacatacc cggtctgcac gtcgccgtac agctcctcga cgcgcatctc
      781 cgtctgctct ggctctggcg ggaacacggg gaggccaccc actcgctgat cgccaaactg
      841 gaactcctgg cgcacctcct ccaccgtcgg ctcgatctcg agatcccccg ccacgatggt
      901 gaccatattg tcgggggcgt accagcgccg gtaatacccc atcagcgttt cccgggagac
      961 gcgcgcgatc acctcttccg tgccgatcac ctcgcgcttg tacgggtggt ggcgatacat
     1021 cagggagttg agcgcctgca ggcagcgccg ccaggggtta tccttgccca tcttgatctc
     1081 ttcgatcacc acgtggcgct cgcgcttctg ctccacctcg ggattggccg ggtcgaacgg
     1141 cggcccgatc tcgttctccg gcaggagcgg gttgaggagc atatcggcat gcagctccag
     1201 ggcacgccgg tagaacctgc cctcttcgcc cttggggagc gtggcgtaat actgcgtcca
     1261 atccttcgag gtactggcgt tgatccgcgc gcccatcccc tccaggatcc ggtcgaaatc
     1321 gccggccggg tagcgaggcg tccccttgaa catcaggtgc tcgaggaagt gcgagatgcc
     1381 gttcgtctcg tcgtcctcca gggccgaccc gacacggact acggtgtgca gatgcaccac
     1441 gtcccccggc ttggggatca ccaccacggt atgcccgcta tcgagcgtcg ccacgcccgc
     1501 cgggcgggcc agatagggca agactacttc tcggatgcct tccttcacat gctcctccta
     1561 cgctgggggt gcaggcagcc ccctgcgaag ccacaccgca cgccgttcac tcgcatccac
     1621 ctgcaggtgg gtttgacggg cagcaggggc agccggcccg gcaaaggcag ccctcaagcg
     1681 cgaggggaac tgccatccgg tcgcacgccg ggtatccgca ctcctacttt tcccttgctg
     1741 agcgcccggt aaactatacc atgcgtgcag gttgacatgc aaggccgcga atcgtctcgc
     1801 ggccttgcat gcggagatgg agcgcgggta tgacgtagcg cgggccagga ataacgtgga
     1861 tgtaaacgcc cgcgccaggc ttcccggcga agctacttgc ccagatggag cgccttgatc
     1921 tcgcccggcg ccagctccag agcaaccggc ttccgcgg
//
LOCUS       DAOSCV010000883         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38645_length_1957_cov_3.410620, whole genome shotgun sequence.
ACCESSION   DAOSCV010000883 DAOSCV010000000
VERSION     DAOSCV010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38645_length_1957_cov_3.410620"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PLY56_17325"
     CDS             <1..849
                     /locus_tag="PLY56_17325"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ23291.1"
                     /translation="NGTGNLFVRLLGMKEPPAHHHLHTVDELKILVDASHKGGMLDET
                     ERELLQRVFRFSDVTTRQVMVHRTQVKGVPVDISFDELAQVIEESPFTRIPVYEGSID
                     NIIGVLHLKDLFRYARKAGPDFCPRSVMHKPLFVPETLSVELLLNEFKQNRTQMAVVL
                     DEFGGTAGVVTLKDVLDEIVGHVHEEYEEDEPEIVEGEDGTIWLQGHVRIDDLNAKFD
                     LALEEPDADTVGGLVMTRLGRMPLVGDVVEENGARFRVEAVHGLRISRVAMQLAQPAA
                     RRNGEL"
     gene            996..1083
                     /locus_tag="PLY56_17330"
     tRNA            996..1083
                     /locus_tag="PLY56_17330"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1030..1032,aa:Ser,seq:cga)
     gene            complement(1426..>1957)
                     /locus_tag="PLY56_17335"
     CDS             complement(1426..>1957)
                     /locus_tag="PLY56_17335"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ23292.1"
                     /translation="GGEFMAPDRIERLQAIAARTYVDDQGHTQPYLTENEVRNLSIPR
                     GTLLPEEFQIIRGHASVSARLLSQIPFSRKLRNVPIFAGDHHEALNGSGYPHGKQGDE
                     LALQSRILAIADVYDALTSSDRPYKKAFSPEVAFDILRKDAAAGKLDGRLVELFIRER
                     CHEKLQEELAAEESEG"
BASE COUNT          353 a          614 c          631 g          359 t
ORIGIN      
        1 aacggcactg gcaacctctt tgtgcgtctc ctcggcatga aggaaccgcc ggcgcaccat
       61 cacctgcaca ctgtcgatga gctcaagatc ctggtcgacg ccagccacaa gggaggcatg
      121 ctggatgaaa cggagcgaga gcttctgcaa cgggtgttcc gctttagcga cgtgacaacc
      181 cggcaggtca tggtccaccg cactcaggtg aagggcgtgc cggtggatat cagctttgat
      241 gagctggcgc aagtcatcga ggaaagcccg ttcacccgca tcccggtcta tgagggcagc
      301 atcgacaaca tcatcggcgt gctccacttg aaggatcttt tccgatatgc acgcaaagcc
      361 gggcccgatt tctgcccccg gagcgtgatg cacaagccgc tcttcgtgcc ggagacgctc
      421 tcggtagagt tgctcctgaa cgagttcaag cagaaccgca ctcagatggc ggtggtgctc
      481 gatgagttcg gcggcaccgc gggcgtcgtc acgctcaagg atgtcctcga cgagattgtc
      541 ggccatgtcc acgaagaata cgaagaagac gagccggaga tcgtcgaagg cgaagatggc
      601 acgatctggc tccagggcca cgtccgcatc gatgacctga acgccaagtt cgatctggcg
      661 cttgaggagc cggacgcgga caccgtgggc ggcctggtca tgacgcggct gggacgcatg
      721 cctctcgtgg gcgacgtggt ggaggagaat ggcgcgcgct tccgcgtgga agccgtgcat
      781 ggcctgcgta tcagccgggt ggcgatgcaa cttgcgcagc ccgctgcccg ccgcaacggc
      841 gagctctgag aacgcctccg gcatcgcgat gctagcccgc gcaggccctc cggccgcgct
      901 gttttcgggc agggcccgac cgggccggtg gggccgcgcg atcagctcgc aggggcttgc
      961 cagaatgcac agagtgtggt agaatcaggc tacccggaga ggtgtccgag tggtttaagg
     1021 tgcagctctc gaaaagctgt gtggcgcaag tcaccgtggg ttcgaatccc accctctccg
     1081 ccagaagggg ctctgcagtc cgcagagccc cttttttgcg tacgctccgc caggcttcgc
     1141 cgcgccgatc catctcccta cggcgtccag gcgcccagcc gccatacttg cgcgactccg
     1201 cgacacctcg ctctgcgcga cggcttcctc gcctttctgc ctggagcgag ggcatggaaa
     1261 ccacgcgttg agtccatcaa caaagagtgg gaactccgcg cgcggaggcg gtgtttatgg
     1321 aagcgaagga cctcatgggc gttgccgggg cagcacaacg cttgggtccg ctccccggcc
     1381 ggcgcgtatc gggtgcggcc ggccggggtc gtttttctct gaggcttacc cttcgctctc
     1441 ctcggcagcc aactcttcct gaagcttctc gtggcaacgc tcgcggatga acagctctac
     1501 cagccggcca tccagcttgc ccgccgccgc gtccttgcgc aggatgtcga aggccacctc
     1561 cgggctgaac gccttcttgt aaggccggtc agacgaagtg agcgcgtcgt agacatcggc
     1621 gatggcaaga atacgtgact ggagcgccag ttcgtcaccc tgcttgccat gcgggtagcc
     1681 gctgccgttg agggcttcgt gatggtcgcc ggcaaagatc ggcacatttc tgagcttccg
     1741 cgagaaggga atctgcgaga ggaggcgcgc cgacaccgac gcgtgcccgc ggatgatctg
     1801 aaactcctcg ggcaacaggg ttcctctcgg aatggagagg tttcgcactt cgttctcggt
     1861 cagatacggt tgcgtgtgac cctgatcatc cacgtaggta cgcgcggcga tcgcctgcag
     1921 gcgctcgatc cgatccggcg ccatgaactc gcccccg
//
LOCUS       DAOSCV010000884         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38700_length_1955_cov_3.136316, whole genome shotgun sequence.
ACCESSION   DAOSCV010000884 DAOSCV010000000
VERSION     DAOSCV010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38700_length_1955_cov_3.136316"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..>1955
                     /locus_tag="PLY56_17340"
     CDS             153..>1955
                     /locus_tag="PLY56_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOJ23293.1"
                     /translation="MRLHSWPLVTALLLAIPAVAAPARNKTLPIPNGDFEQGAQGWNI
                     PADEGMSTLSQEQAASGKWSLKVVDDHPKNGSSAIASRVPIEGAGVYELHGKAFPVSG
                     SGLGIYVRVLDKEGKLIGKGDDFHRAAPSEPVGKWVPFTSTIYAPEGAAYLELWIHSY
                     SHAKVVAYLDDFRFEALDPKAMRPPWEGTYKIRPEEKAKLTAADVVGPDGIVYPDWCW
                     AGVPGGIPKVPVVARIEEFGAKADDDADDSAALERGAEAVGKRGGGALLLGAGTYYLD
                     RPVLITRDGVVLRGAGAEKTRLIFRYGVPAGGVRFCRPAPGATVTPTTAIEIHADPKE
                     LQAISLEVDGQEVRRIQRHAHWGGTFSLSVAGSAVTARAGSGEHILKAVADYPGGRKA
                     EAMLRVRTDASTKDGRLHTPPAAAIAFSGGAYTSPQIKLARDGRRGDRELLLESTAGI
                     TAGDRIRLRAPKTPRWDALVRNACQWGEYRRTELVVERVDGQRIIMNQPLRIDFPVVD
                     GAYVQVIHPIRRCGVEDLSLEQREPIWTSGIYFHNVWESWARGVVVKKAGRFPLYFTG
                     AKWCEIRDCVLDDAWFKGGGGSAYAGWEQACDCLM"
BASE COUNT          324 a          625 c          692 g          314 t
ORIGIN      
        1 ctggtagatg gccagatgca caccttcctc ttccgcgaga cgcgctcgct gctcgactct
       61 cgctcgcggt tgctgacgtt gcgctgcctg acggaggatt gacgccagag cttccctcga
      121 gttcctcgcg ctggggtacc gtggaggctg tgatgagact tcactcttgg cccctcgtca
      181 ccgcgttgct tctggcgatc ccggcggtcg ctgcgccagc gcggaacaag acgctcccga
      241 tacccaacgg agacttcgag cagggcgcgc aagggtggaa catcccggcg gatgagggga
      301 tgtccacgct gtcgcaggag caggcggcgt ccgggaaatg gtcgctcaag gtggtggacg
      361 accatcccaa gaacggctcc agcgccatcg ccagccgcgt acccatcgag ggagcaggcg
      421 tctacgaact gcatggaaaa gccttccccg tttctggatc cggcctcggg atctacgtgc
      481 gcgtgctcga caaagaggga aagctcatcg gcaagggcga cgacttccac cgcgctgcgc
      541 cctccgagcc cgtggggaag tgggttccct tcacaagcac catctatgcc cctgaaggcg
      601 ccgcctatct ggagctgtgg atccatagct acagccacgc gaaggtggtg gcctacctcg
      661 atgacttccg gttcgaggcc ctggatccga aggcgatgcg gcctccttgg gaaggcacct
      721 acaagatccg gcccgaggag aaggcaaagc tgacggcagc cgacgtggtg ggtcccgacg
      781 gcatcgtcta ccctgactgg tgctgggccg gcgtgcccgg cggcattcca aaggtgccgg
      841 tggtggcgag aatcgaggag ttcggcgcga aggcggatga cgatgccgac gacagcgccg
      901 ccctggagcg cggcgccgaa gcggtcggga agcgcggcgg gggcgccctt ctcctgggag
      961 ccggcactta ctacctcgac cggccagtgc taatcacgcg ggatggcgtc gtcctgcgcg
     1021 gcgccggcgc cgagaagacg cggctgatct tccgctacgg cgtgccggcg ggcggcgtgc
     1081 gtttctgccg gcccgctcca ggcgcgacgg tgacccccac caccgccatc gagatccatg
     1141 ccgacccgaa ggagctgcag gcgatctcgc tggaggtgga tggccaggag gtgcgccgca
     1201 tccaacggca tgctcactgg ggcggcacct tctccctgag cgttgctgga agcgcggtga
     1261 ccgcgcgggc cgggagcggc gagcacatcc tcaaggcggt tgccgattac cctggcggca
     1321 ggaaggccga ggcgatgctc cgggtgcgca ccgacgcgtc cacaaaggac ggccgcctcc
     1381 atacgccgcc ggctgcggcg attgcattca gtggcggcgc gtatacgagc ccgcaaatca
     1441 agttggcccg tgacggccgc cgcggtgacc gggaactcct cctggagagc accgcgggga
     1501 tcaccgcggg cgaccggatc cgcctgcgcg cgcccaagac tccgcgatgg gacgccctgg
     1561 tccgcaacgc ctgccagtgg ggcgagtacc ggcgcaccga gttggttgtc gagcgggtgg
     1621 acgggcagcg gatcatcatg aatcagccgc tgcgcatcga ctttcccgtg gtggatggtg
     1681 cctatgtgca ggtgatccac ccgatccggc gctgcggcgt cgaggatctc tccctggagc
     1741 agcgagagcc gatctggacg agcgggattt acttccacaa tgtctgggag agctgggcgc
     1801 gcggcgtggt ggtgaagaag gccgggcggt tcccgctcta cttcaccggc gcgaagtggt
     1861 gcgagatccg ggattgcgtc ctcgacgacg cctggttcaa gggcggcggt ggcagcgcct
     1921 acgccgggtg ggagcaggcc tgcgattgtc taatg
//
LOCUS       DAOSCV010000885         1955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38708_length_1955_cov_2.564737, whole genome shotgun sequence.
ACCESSION   DAOSCV010000885 DAOSCV010000000
VERSION     DAOSCV010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38708_length_1955_cov_2.564737"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLY56_17345"
     CDS             <1..414
                     /locus_tag="PLY56_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOJ23294.1"
                     /translation="GVLFGREDCGLSTRDLTHCQAIATIPAAAGMPSLNLSHAVQVVA
                     YEIFQASLGKMLCKQADLADIAEQEALIRRVNDLMLSTGFRPFRDDPEAFAHTLRRVF
                     GRAGMERRDVRALHKLIATLQHCVATGPVGDGEEP"
     gene            501..872
                     /locus_tag="PLY56_17350"
     CDS             501..872
                     /locus_tag="PLY56_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOJ23295.1"
                     /translation="MSRATSTTVGVLVGGAVGAIAALLLAPKRGEEMRSDLMQWTRDR
                     EHKAAERIRQAGASMASNLRQMPSKVSQKAPELIERAKSRFQRGAEESAHMMESATEK
                     VAESMEKGSEQEHSSQQVPIT"
     gene            complement(980..>1955)
                     /locus_tag="PLY56_17355"
     CDS             complement(980..>1955)
                     /locus_tag="PLY56_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23296.1"
                     /translation="RRGVFYGDVGMRFEIPELPPGGQAMLLADGDGESLESGYTLTLS
                     RNGEGAALILAHGGRELARATLPGAGPLALSLRKQGTRVLGLVDDKVVVKAPAAAQAG
                     GGRLAFRAAGFKPRISSIAVWSANVSDYTFDTAPVDWWVASGEWDVTNRWSCTPDWSW
                     FGGFSAGVAAIWHKRPFEGNIALDFYAGPKMITLDNERSKEAMADFNAALCGDGRDVN
                     SGYAFIVAENRTGARILKQGKVVAENRAFRLFTHGHNRWANIRVEKHGGRLRLFVEGQ
                     LTVEYTDPQPLSGGYAGIWTRNNGIMVPRVTLYHEALGERLLSLPWRR"
BASE COUNT          330 a          629 c          658 g          338 t
ORIGIN      
        1 ggcgtcctct tcgggaggga agattgtggt ctctctacgc gcgacctgac gcactgccaa
       61 gcgatcgcga cgattcctgc ggcggcggga atgccgtcgc tcaacctctc gcacgcggtg
      121 caggtggtgg cgtatgagat ctttcaggcc agtttgggaa agatgctgtg caagcaggcc
      181 gatctggctg atattgccga gcaggaggcg ctgatccggc gcgtgaacga cctgatgctc
      241 tcgaccgggt tccggccgtt tcgcgatgat ccggaggcgt ttgcgcacac gctgcgccgc
      301 gtgttcgggc gcgctggcat ggagcgccgc gacgtgcgcg cgttgcacaa gctgatcgcg
      361 acgctgcagc attgcgtcgc caccgggccg gttggagacg gcgaggagcc ataggccggc
      421 gaggcctctc tcctccggtt ttgccacgga gtgcctgggt aaatggactt gggcgtgcag
      481 tgccccgaga ggagaggaag atgagcaggg ctacgagcac aacggtggga gtgcttgttg
      541 gcggcgccgt tggcgctatt gccgcccttc tcctggcccc gaagcggggt gaggagatgc
      601 gctccgacct catgcaatgg acacgcgata gggagcataa ggctgcggaa cggatccggc
      661 aggcgggcgc cagcatggca tcgaacctcc ggcagatgcc gtcgaaggta tcgcagaagg
      721 cgcccgagct gatcgagcgc gcgaagagcc gcttccagcg cggggctgaa gaatcggccc
      781 atatgatgga gtcggccacc gagaaggtag ccgagagtat ggagaaaggg agcgagcagg
      841 agcacagctc tcagcaggtc cccattacat aacagcagcc gcgccgggag ggttactcta
      901 cctgagccgg gcaggtgttt cctttgtcct gcccggctcc tgctgttttc cccctcttcg
      961 cgccacgcgt ctcctctcgt catctcctcc aggggagcga gaggagtcgc tcgccgagag
     1021 cctcgtggta gagcgtgacg cgcggcacca tgatgccgtt gtttcgagtc cagatgcccg
     1081 cgtagccgcc agagagcggc tgcggatcgg tgtactccac ggtgagttgg ccctccacga
     1141 agaggcgtag ccgaccgccg tgcttctcca cgcgaatgtt ggcccagcgg ttgtgcccgt
     1201 gggtgaacag ccggaacgcg cggttctccg cgactacctt cccctgcttc aggatccggg
     1261 cgccggtcct gttctccgcg acgatgaagg cgtagccgct gttgacatcc cggccatcgc
     1321 cgcacagcgc ggcgttgaag tcggccatcg cctccttact gcgctcgtta tccagcgtga
     1381 tcatcttcgg accggcgtag aagtcgagcg cgatgttccc ctcgaacggg cgcttgtgcc
     1441 agatcgccgc gacgcccgca ctgaacccgc caaaccacga ccaatccggc gtgcagctcc
     1501 agcggttcgt gacgtcccat tcgccactgg ccacccacca atccacgggc gcggtgtcga
     1561 aggtgtagtc gctgacgttg gccgaccaga ccgcgattga cgagatgcgc ggcttgaacc
     1621 cggccgcgcg gaaggcgagc cggcctccgc cggcctgagc ggccgccggt gccttcacga
     1681 ccaccttatc atctaccagg ccaagaacgc gagtgccttg cttgcgcagg ctgagggcga
     1741 gcgggccggc gccagggagc gtggctcttg cgagctccct tccaccgtgc gccaggatga
     1801 gcgccgcgcc ctcgccgttc cgcgagaggg tgagcgtgta gccgctctcc agagactcac
     1861 catcgccatc ggcgaggagc attgcctggc ctcctggagg gagctcgggg atctcgaagc
     1921 gcatccccac gtcaccatag aaaacgcccc gccgc
//
LOCUS       DAOSCV010000886         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38900_length_1948_cov_3.331220, whole genome shotgun sequence.
ACCESSION   DAOSCV010000886 DAOSCV010000000
VERSION     DAOSCV010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38900_length_1948_cov_3.331220"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..>1948
                     /locus_tag="PLY56_17360"
     CDS             99..>1948
                     /locus_tag="PLY56_17360"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ23297.1"
                     /translation="MLFNHSIIRLTIALFATLALSIATAMAAPAGRRDAVVLVEGGNP
                     RAAVVVPATADDQTKAAAELLIRYVEKATGAKLPLHAEGAKEAGALPARVFVGPCQAV
                     TQAKLLPEGMDGDGFVIAPVGRDALAIVGPTPWGTEFGVCEFLERYAGVRWLMPGPDG
                     EDVPRAKDLAAARETIRQEPAFFSRLFSGLRGPAQVEWARRNRMHGRVSFHHNLIHLF
                     PPEKYTATHPEFFPIRNGKRFLPKDSTVHGWQPCFTAEGIVEEAIRNINAYFDEHPEA
                     TSYSLGVNDSSGHCECERCQARDPGGNNFLGRRDVSDRYFEWANKVVEGVLKKHPDKF
                     FGCLAYSEVAAPPTRVRVHPRIIPYMTYDRMKWADPEIRAEGEEMTRAWHRASPTVGW
                     YDYLYGTPFCLPRVYFHRMAEYYRFARASGVKVHYAEAYPNWGEGPKLYISLKLQWNP
                     DQDVDQLLREWYDRAVGPAAAPELAAYYAHWEKFWTERALKSRWFSRGGQYLNFTSPL
                     YLADVTASEIAESRRRLEAVVAKAQTPAQKARARLLLQAFEYYEASALAYPKVEAGDA
                     KPPASEAEALAMIAEGERQTKMAEKRIRLAEEWEKDPVLAHPLSPARFPLL"
BASE COUNT          349 a          646 c          648 g          305 t
ORIGIN      
        1 gagcccgccg ccagggagat caccgcaggg cggcgggcca tgcccgggca tcggagtgcc
       61 cgcccggcgt ccgatgatcc ggcaccgagg agagagagat gctgttcaac cattccatca
      121 tacgattgac catcgccctc ttcgccaccc tggcgctctc tatcgccacc gccatggcgg
      181 ccccggcggg gagaagggac gcggtcgttt tggtcgaggg aggaaatccg cgcgccgccg
      241 tcgttgtgcc cgccaccgcc gatgaccaga cgaaggcggc cgccgagctc ctgatccgct
      301 atgttgaaaa ggcgaccggc gcgaagctgc ccctccacgc cgagggtgcg aaggaagccg
      361 gtgcgctccc agcgcgcgtc ttcgtcggac cgtgccaggc cgtcacgcaa gcgaaactcc
      421 ttcccgaggg aatggatggc gacgggtttg tgattgcgcc tgtcggcaga gacgcgctcg
      481 ccatcgtggg cccaacgccg tggggcaccg agtttggcgt ctgcgagttc ctggagcggt
      541 atgccggcgt gcgctggctg atgccgggtc cggacggcga ggacgtgccg cgagcgaagg
      601 atctcgcggc agcgcgcgag acgattcgcc aggagccggc cttcttctcg cggcttttca
      661 gcggcctgcg cggtccagca caggtcgaat gggcacggcg aaaccggatg catgggcggg
      721 tgagcttcca ccacaacctg atccacctct tcccgccgga gaagtacaca gcgacgcatc
      781 cggagttctt tccgatccgc aacggcaagc gcttcctgcc aaaggacagc acggtccacg
      841 gctggcagcc ctgcttcacc gccgagggga tcgtcgagga ggcgattcgg aacatcaacg
      901 cctactttga cgagcatccg gaggcgacat cctactccct gggcgtcaac gacagcagcg
      961 gccactgcga atgcgagcgc tgccaggcgc gcgatccggg tggcaacaac ttccttggcc
     1021 ggcgcgacgt ctccgaccgc tacttcgagt gggcaaacaa ggtggtcgag ggcgtcctga
     1081 agaagcatcc ggataagttc tttggctgcc tcgcttactc ggaggtcgcg gcgcctccca
     1141 cgcgcgtgag agtccatccg aggatcatcc cctacatgac gtacgaccgg atgaagtggg
     1201 ccgaccccga gatccgggcc gagggcgagg agatgacccg ggcgtggcac cgcgcaagcc
     1261 caactgtcgg ctggtacgac tacctctacg gcaccccctt ctgcctcccg cgagtctact
     1321 tccaccgcat ggcagaatac taccgttttg cgcgcgccag cggggtgaaa gtccattacg
     1381 ccgaagccta ccccaactgg ggcgagggcc cgaagctgta catctcgctc aagctgcagt
     1441 ggaacccgga ccaggatgtc gaccagctgc tgcgcgagtg gtacgatcgc gccgttgggc
     1501 cggccgcggc gccggagctc gccgcctact acgcgcactg ggagaagttc tggaccgagc
     1561 gcgcgctgaa gtcgcgctgg ttctcgcgcg gcgggcaata tctgaacttc acctcgccgc
     1621 tctatctggc ggacgtcacg gcgtctgaga tcgctgagag ccggcgccgc ctggaggccg
     1681 tcgtcgcgaa ggcgcaaacc ccggcgcaga aagcacgggc gcgcctgctc ctccaagcgt
     1741 tcgagtatta cgaggcgtcc gcgctcgcct atccgaaggt ggaagcggga gacgccaagc
     1801 caccggcttc cgaggccgag gcactggcga tgattgccga gggagaacgc cagaccaaga
     1861 tggcggagaa gcggatccgg ctggcggaag agtgggagaa agatcccgtg cttgcccacc
     1921 cattgagccc tgctcgcttc ccgcttct
//
LOCUS       DAOSCV010000887         1948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_38913_length_1948_cov_2.649234, whole genome shotgun sequence.
ACCESSION   DAOSCV010000887 DAOSCV010000000
VERSION     DAOSCV010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38913_length_1948_cov_2.649234"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PLY56_17365"
     CDS             <1..1362
                     /locus_tag="PLY56_17365"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23298.1"
                     /translation="FTCSMYPDQEPGFLKLVEAELAHNIKRLSNYTCLALWCGENEAH
                     WGWEEWWTELREQFPRFCGADIFDAMFVLLLAELDPDRFYWNGSPYSGEPEVRANHPH
                     FGDLHLWTLHTNTEDFHRFTSVQPSFVSETGIQSFPDLRTALSIGTEEGHDLLSFVMD
                     TRNHFESTAKNERLLKFVGALFRRNCDFAHDVVLSNLAHAEYLKYAVEYWRSLAYNCG
                     GVLIWQLNDCWPAISWAAVDYNLIPKACHYYLKRAFEPDLVGWVQHFSKDHRPEVNSR
                     GDLFVASERDGHKSGVVELRVVRVTGELVYCHQYPVTLDGRGVAALGEVVLPDYRHTR
                     FDCVAEVTLRWEDGRTARNLYTFSRPKHMRLPRPDIALEQAAPDRLRVRTDLFAKGGY
                     LFHRDPGVIFGDNYFDLLPGEEREVSLSRAAAAEEGQVLGYHHGDSDAPSRQAPRLEH
                     LHP"
     gene            1454..>1948
                     /locus_tag="PLY56_17370"
     CDS             1454..>1948
                     /locus_tag="PLY56_17370"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOJ23299.1"
                     /translation="MINRLTLDGTWELAGFDEGTGDWAWPEDRYPIPAKVPGEVHPAL
                     MAAGLIPDPYYGDNASRASWVEEKEWWYRRRFRLPEGFLHPRAFLEFDGLDTFATVLL
                     NSRQVGESRNMFIPYRFDVTGMLREEGENVLEVRFASTARVAGRMDLDDLEVLYRRDR
                     AGVRK"
BASE COUNT          320 a          629 c          650 g          349 t
ORIGIN      
        1 ttcacctgct ccatgtaccc ggaccaggag ccggggttcc tgaagctggt ggaagctgag
       61 ttggcgcata acatcaagcg gctcagtaac tacacctgcc tggcgctgtg gtgcggcgag
      121 aacgaggccc attgggggtg ggaggagtgg tggacggagc tgcgcgagca gttcccccgg
      181 ttctgcggcg ccgatatctt cgacgccatg ttcgtgctcc tgcttgcgga gctcgacccg
      241 gaccgcttct actggaacgg ctccccctat tcgggcgagc ccgaggtgcg cgccaaccac
      301 ccgcacttcg gagacctgca cctctggacg ctgcacacca acacggagga cttccaccgc
      361 ttcaccagcg tgcagccgag cttcgtcagc gagacgggca ttcagtcgtt ccccgacctg
      421 cgcacggccc tcagcatcgg tacggaggag ggccacgacc tcctctcttt cgtgatggat
      481 actcgaaatc acttcgagag caccgccaag aacgagcggc tgctcaagtt cgtcggcgcc
      541 ctctttcggc ggaactgcga cttcgctcac gatgtggtcc tctccaacct ggcgcatgcg
      601 gagtatctca agtacgcggt cgagtactgg cgctcgctcg cgtacaactg cggcggcgtg
      661 ctcatctggc agttgaacga ttgctggccc gcgatctcct gggccgcggt agattacaac
      721 ctgatcccca aggcatgcca ctactatctg aagcgcgcgt tcgagccgga tctggtgggg
      781 tgggtccagc acttctccaa ggaccatcga cccgaggtca actcccgtgg cgatctcttc
      841 gtcgcctccg aacgcgacgg gcacaagagc ggcgtggtag agctgagggt cgtgcgcgtg
      901 acgggcgagc tggtctattg ccaccagtat cccgtcacac tcgacgggcg aggggtggcg
      961 gcgctgggtg aggtggtcct gcccgactac cggcatacgc gcttcgattg cgtcgcggag
     1021 gtcacgctgc ggtgggagga tggtcgcacg gcccggaatc tctacacctt ctcgcggccc
     1081 aagcatatgc gtctgccgcg acccgatatt gccctggagc aggccgcacc ggaccggctg
     1141 cgagtgcgca ccgatctatt cgccaagggg ggctacctct tccatcgcga ccccggcgtc
     1201 atcttcggcg acaactactt cgacctgctg cccggcgagg agcgcgaggt gtcgctctct
     1261 cgcgcggcgg cagccgaaga ggggcaggtg ttgggctacc accacgggga ttcggatgcg
     1321 ccgagccgac aggccccacg gttggagcat ctccacccct agagagttgc acggctccgg
     1381 gtatgaccac gccggtgagg gtacggggtg ccggcagtcc ggcgcacccc gctctcgcgg
     1441 gaggggagac aggatgatca accggttgac gctcgatggt acgtgggagt tggcaggctt
     1501 cgatgagggg accggcgatt gggcatggcc cgaggaccgc tatcccatcc cggccaaggt
     1561 acccggagag gtccaccccg cactgatggc ggccggcctg atccccgacc cctactacgg
     1621 ggacaacgcg tcgcgggcgt cgtgggtgga ggagaaggag tggtggtacc gccggcgctt
     1681 tcggctcccc gagggcttcc tgcacccgcg ggccttcctg gagttcgatg gcctcgatac
     1741 tttcgcgacg gtgctcctga acagccgcca ggtgggcgag tcgcgcaata tgttcatccc
     1801 ctaccgcttc gacgtgaccg gcatgctgcg cgaggagggt gagaacgtcc tggaggtgcg
     1861 ctttgcctct acggcccgcg tggccgggcg catggacctg gatgacctgg aagtcctcta
     1921 ccgccgcgac cgtgccggtg tgcgcaag
//
LOCUS       DAOSCV010000888         1944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39024_length_1944_cov_3.389624, whole genome shotgun sequence.
ACCESSION   DAOSCV010000888 DAOSCV010000000
VERSION     DAOSCV010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39024_length_1944_cov_3.389624"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    272..281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            546..1568
                     /locus_tag="PLY56_17375"
     CDS             546..1568
                     /locus_tag="PLY56_17375"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOJ23300.1"
                     /translation="MRRVSKCAFIALLAATLVAPAGAAPEERVVIDGYLQARAVVTER
                     PSGVYDIEGSTFTIRRARVRFRHATEIARFVVELEGTARTGPVIRDAYVQIPFHIGQA
                     RGPFTFWVGQFQIPFGYAVPSSSRQNLFPELPVGFVEDTVGLALFPDLRDVGLRLSFE
                     MGRTQADLAVVNGSGLADADPNRSKEFYARIKRRFGSLEAALSGRIGNQVIAGVDRQH
                     SIWGIGAKVTPSFGGEFTAEFATGRDAATATSHTTPRTWYIEYDRPLGGGWLVGARYS
                     SFDPDIDNVGVAGNTGEERGAAFALAYQFDEHLRLTGALEFPRVTTSLGRQTATAGIF
                     QTQYSF"
     gene            1597..>1944
                     /locus_tag="PLY56_17380"
     CDS             1597..>1944
                     /locus_tag="PLY56_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOJ23301.1"
                     /translation="MQQTIRIATRGSALARTQAAWVAERLTMHHPNLKVETHIIKTLA
                     DAHQEIPLHGFRQRGVFVKEIENALLSGEANLAVHSMKDLPADIPDGLVVGAVPPRED
                     PLDVLVLRDEPAPL"
BASE COUNT          330 a          664 c          611 g          329 t
ORIGIN      
        1 gctcacgcgc agggcatcgg tgaggtgagg cccggcgacg taccccttcg ccgcgatatg
       61 ggccaccggc atccacaccg tccacagggc atcgcgcaaa cgcgccttca tcccttctgg
      121 cgagcggcgg tcgctcgatc ccgaaactcc cgccttcgat ttgcgatctc ccatcgtggc
      181 tgcgctcctt gccagatccg gcgagcaacc accttggcgc aaaaaccatg ccaaccgcgt
      241 ggcggcctcc acgcgcgcgt tccccgaacg cnnnnnnnnn nccgatttgt ggtataatgc
      301 ctgcgcccca ccgggcgttt cagctccccg aaaagtatca cccccgtgaa aacaggcgct
      361 cgggcaactt ccggggaggc acgagggatc gcgctcctcg gtagaggagg ccgcgtgcag
      421 gcagcacgcc tggtggaata tgcgcgcgct ttcggggaac tgcttcgggg tgccaacacc
      481 ccccaggaac ggcacgccgc ccgaccggaa gagcgccccc cgcggcatct ggagagagga
      541 ttgggatgcg aagagtcagc aagtgcgcct tcattgccct tctggctgct acgctcgttg
      601 ctccggccgg cgcggcccca gaagagcgcg tagtgatcga tggctatctc caggcacgcg
      661 cggtcgtcac cgagcggcct tccggagtgt acgatattga ggggagcacc ttcacgatac
      721 gccgggcgcg ggttcgcttc cggcacgcga cggagatcgc tcgcttcgtg gtggagctcg
      781 agggcacggc gcgcacgggt cccgtaatcc gcgatgccta cgtgcaaatc cccttccaca
      841 tcggccaggc gcgcgggcca ttcaccttct gggtaggaca gttccagata ccattcgggt
      901 atgccgtgcc gagcagctcg cgccagaatc tctttcccga gctacccgtg ggcttcgtgg
      961 aggatacggt tggtctcgcg ctcttccccg atctgcgcga tgtgggccta cgcctctctt
     1021 ttgagatggg gcgcacgcag gcagatctcg cggtcgtcaa tgggtcgggg cttgccgacg
     1081 ccgatcctaa tcgcagcaag gagttctacg cgcgcatcaa gcgccgcttc ggatccttgg
     1141 aggcggccct ctccggacgc atcggcaacc aggtgatcgc cggcgtagac cgccagcaca
     1201 gcatctgggg gatcggcgcg aaggtaacgc cctccttcgg tggcgagttc accgccgagt
     1261 ttgccaccgg gcgggatgcg gccactgcca catcgcacac cacgccgcgc acatggtaca
     1321 tcgagtatga ccgccccctg gggggcgggt ggctcgtggg cgcacgctac tcctccttcg
     1381 acccggatat tgacaatgtt ggcgtcgctg gcaacaccgg cgaggagcgt ggggcggcgt
     1441 tcgcgctcgc ctatcaattt gacgagcatc tgcgcctgac cggtgctctg gagtttcctc
     1501 gcgtcaccac atcgcttggc cggcagacag cgaccgcggg catcttccag acccagtact
     1561 ccttctgagc ggacggccgt aaacaggggg gagaggatgc agcagacgat ccgaatagcc
     1621 acccggggca gcgccctggc acgcacccag gccgcctggg tcgccgagcg cctcaccatg
     1681 caccacccga acctgaaggt ggaaacgcac atcatcaaga cgctggcgga cgcgcaccag
     1741 gagatccccc ttcacggctt tcgccaacgc ggcgtcttcg tcaaagagat cgagaacgcg
     1801 cttctcagtg gcgaggccaa cctggccgtc cactcgatga aggacttgcc ggccgacatc
     1861 ccggatggcc tcgtcgtggg cgcggtaccc cctcgggagg accccctcga tgtgctggtc
     1921 ctccgcgatg agccggcccc tctt
//
LOCUS       DAOSCV010000889         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39065_length_1943_cov_1.673199, whole genome shotgun sequence.
ACCESSION   DAOSCV010000889 DAOSCV010000000
VERSION     DAOSCV010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39065_length_1943_cov_1.673199"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLY56_17385"
     CDS             <1..243
                     /locus_tag="PLY56_17385"
                     /inference="COORDINATES: protein motif:HMM:NF017806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HOJ23302.1"
                     /translation="GPVLRRQGNEPVRTDNGNYLLECHTGPIENPEELARSLEGIAGV
                     VGHGLFLGLATTAIIGSDQGTRILQITPDKDEAPRM"
     gene            complement(267..653)
                     /locus_tag="PLY56_17390"
     CDS             complement(267..653)
                     /locus_tag="PLY56_17390"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOJ23303.1"
                     /translation="MGAWRWAPPLAAAAAVVIAALKLLAPYGALPPCLFRMLTGWPCP
                     SCGGTRCVLALTALDLPGAAANNPLVLLAAVGLAVALLLDAGERILHRPLLSPEPLLR
                     RPAVARTLAVALVLANWVYLILAERG"
     gene            complement(656..1390)
                     /locus_tag="PLY56_17395"
     CDS             complement(656..1390)
                     /locus_tag="PLY56_17395"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOJ23304.1"
                     /translation="MEQHCPSCGADLPPEERPSACPVCGADLQPEEGEEIPFDNPGRL
                     GFFRSLWQTWREATFTPNAFFPRIPTSAAVTWPLLYAVLFGWLQALANVLIQQPMLRA
                     SRATLPPQLRNMAEAFQTTPEAVAMQFILEPFITVVVILLSSAVFHAILYLLNGARPE
                     FVITVRTVSYTAGAYAACVIPVLGPLLYVIGVLTLLIIGLARAHQTEGWRSALAVLLP
                     AVACCGAMGMLMLFTAAFLLRATNVM"
BASE COUNT          371 a          642 c          658 g          272 t
ORIGIN      
        1 ggacccgtcc tgcgccgcca ggggaatgag ccggtgcgca cggataatgg caactacctg
       61 ttggaatgcc acacgggccc tatcgagaac cccgaggagc tggcgcgctc gctggagggg
      121 attgcggggg tggtcggcca tgggcttttc cttggcctcg ccacgacagc gatcatcggc
      181 tccgaccagg gcacgcgcat cctgcagatc acgcccgaca aagacgaagc gcctcgcatg
      241 tgagcggggc gctggcccct tgttcctcac ccgcgctcgg ccagaatgag ataaacccag
      301 ttggccagca cgagggcaac cgcgagcgtg cgcgccaccg ctggccggcg caacaaaggc
      361 tccggggaga gcagcgggcg atggaggatg cgctcgcctg catccaggag gagcgccact
      421 gccaatccga ccgcggcgag cagcaccagg gggttgttcg ccgcagcgcc cggcagatcc
      481 agcgcggtga gggccagtac gcagcgcgtc ccaccgcagc tcgggcacgg ccacccggtg
      541 agcatgcgaa agaggcacgg cggcaacgcc ccgtagggag cgagcagctt cagcgcggcg
      601 atgacgacgg ccgccgcagc agccagcggg ggagcccagc gccaggcgcc catcgctaca
      661 tcacgttggt cgcgcgcagc aggaacgccg cggtgaagag catcagcatc cccatcgcgc
      721 cgcagcacgc gaccgcgggg aggagcaccg ccagtgcgct ccgccacccc tccgtctggt
      781 gagcgcgcgc caaaccgatg atcagcaagg tgaggacccc gatgacatag agcagcgggc
      841 cgagcacggg gatgacgcag gccgcgtagg cgcctgccgt atagctcacc gtgcgcacgg
      901 tgatgacaaa ctcgggccgc gccccattga gcaagtagag aatcgcgtgg aaaacggccg
      961 agctgagcaa gataacgacc acggtaatga acggctcgag gatgaactgc atggccacgg
     1021 cctcgggggt ggtctgaaac gcctccgcca tgttgcggag ctgggggggc agcgtggccc
     1081 tgctggcccg cagcatcggc tgctgaatca ggacgttcgc gagcgcctga agccatccaa
     1141 agagcaccgc gtagagaagg ggccaggtca ccgccgctga agttgggatg cgcgggaaga
     1201 aggcgttcgg cgtgaacgtc gcctcgcgcc aggtctgcca cagcgatcgg aagaacccga
     1261 ggcgccccgg gttatcaaac gggatctcct cgccctcttc cggctgcagg tcggcgccac
     1321 acaccggaca tgcggatggc cgttcttccg ggggaagatc tgccccgcag cttgggcagt
     1381 gttgttccat gctttccctc cacgcgccgc aggctcgatt cacgccggca gaggagcaag
     1441 cctgccgccc tgagtgctgc cggctagagt gcggctcccc ccaattatac acgggtttct
     1501 ctccttctgc caagcgcatg cccgcgtggg cctgggattc cgagataaga caggtccggg
     1561 cggttgcccg cgctccccaa ggaggtctcg aacctcggga tcaggaccga cctgctctcc
     1621 aggatccctg ggtgtcgtcc ggtccctgct tccgcggtat cgtcctgggg gtgcaccccg
     1681 ggagagccca cgacctccgt gcgctttcaa gattcagagt agcgtctcag cggcagacgt
     1741 ggtatgatgg gggtacatcc gaccgtagcg cgcctgccgc aggcgtagag aggaaacgcg
     1801 cttggcacgc aagcaatgga acaccccgcc ggcgatgcag atcgacccgg cgaagaagta
     1861 tcgggccacc atcgtcacga accggggcac catcgagctg gagctctccc ccgagttcgc
     1921 gccgaagaca gtgaacaact ttg
//
LOCUS       DAOSCV010000890         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39183_length_1939_cov_2.837580, whole genome shotgun sequence.
ACCESSION   DAOSCV010000890 DAOSCV010000000
VERSION     DAOSCV010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39183_length_1939_cov_2.837580"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLY56_17400"
     CDS             <1..388
                     /locus_tag="PLY56_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23305.1"
                     /translation="TAFVPYASIGEKAYLGGAQVSGSATTISKVKAVWQYEDLFDGGK
                     FRIRAVVPVSALNPLEAKRVDGVGITAVVDMALAEQETLEARRERERRERLQERLAAE
                     RDRERHRHDNGKHKGWYKGKRKGHHK"
     gene            584..1171
                     /locus_tag="PLY56_17405"
     CDS             584..1171
                     /locus_tag="PLY56_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOJ23306.1"
                     /translation="MGLRDVSIGDAAPHEVNTIVEMPKGSSNKYEYDPERDVFVLDRA
                     LYSALFAPADYGYVAQTLAEDGDALDILVVTTFPTFPGCVVAARPVGVLLMCDEKGKD
                     EKILAVSARDPRFNAVELLEDMPDHLLREITHYFRTYKELEDKATQVLDWHGRERALA
                     FIEECHARFLAGGERVPTGEACVFAEPGHPADATS"
     gene            1405..>1939
                     /locus_tag="PLY56_17410"
     CDS             1405..>1939
                     /locus_tag="PLY56_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23307.1"
                     /translation="MKISSDWHIHTRNSCDSASLVVSELIPAAEALRIEAFGISDHIH
                     TPYNLPDLVASRREFDESQPPPNFHFGVEVSCVSQWELDEIATGKHANPVYGLRQGGP
                     AWGPLAIGIGAEELKRLGVEYVIGGTHWPMYLPADRDAIIRDYHRQNLFLATHPLVTI
                     VAHPWWWMGYWKDDDGCY"
BASE COUNT          356 a          600 c          657 g          326 t
ORIGIN      
        1 gaccgccttc gtgccctatg ccagcatcgg agaaaaggcg tacctcgggg gcgcccaggt
       61 ctccggttcc gcgaccacca tctcgaaggt gaaggctgtc tggcagtacg aggacctgtt
      121 cgacggcggc aaattccgga tccgggccgt tgtgcctgta tcggcgctca acccgctgga
      181 agcgaagcga gtcgatggcg ttggcatcac cgcggtggtt gatatggcgc tggccgagca
      241 ggagactctg gaagcccgca gggagcgcga gcgccgcgaa aggctgcaag agcgcttggc
      301 ggcggagcgc gaccgggagc gccaccggca tgataacggc aagcacaagg gttggtacaa
      361 gggaaagcgc aaggggcacc ataaataagc acgacgactg agcccctcgc gggatgtgcg
      421 cggcggccgc cacggagtgc cttcgcgtca caaccctggc tgcagcgtgt tccctaagag
      481 ttcagaccgc caggtacccc gggtgcctgg cggtcgcgat tcggaggttt gggtctcgcc
      541 agcggagcgc atacgtatag caggaccggc aggggggaac gagatgggcc tgcgagatgt
      601 ctccattggc gatgccgcac cgcatgaggt gaacacgatt gtcgagatgc ccaaggggag
      661 ctcgaacaag tacgagtatg acccggagcg cgacgtcttt gtccttgacc gcgcgctcta
      721 ctcggcgctt ttcgcgccag ccgactatgg ctacgtggcg cagacgcttg ccgaggatgg
      781 cgacgcgctc gatatcctgg tggtgaccac ctttccgacc ttccccggct gcgttgtggc
      841 cgcccggccg gtgggagtcc tcctcatgtg cgacgagaag gggaaggatg agaagatcct
      901 cgccgtctcc gctcgcgacc cccgcttcaa cgccgtcgag ctgctcgagg atatgccgga
      961 ccatctcctc cgcgaaatca cccactattt ccgcacctac aaagagctcg aggataaggc
     1021 gacacaggtc ttggactggc atggtcgcga gagagcactc gcgttcatcg aggaatgcca
     1081 cgcccggttc cttgccggtg gagagcgcgt gcccacaggc gaggcatgcg tctttgccga
     1141 gcccggccat ccggcggatg cgacctcgtg agagctagca agccccggtg ttgattgcga
     1201 ttccggaagg cgcacccggt tctgccacgt ggggcgcgca actgcgcgga atctcagccg
     1261 gacgtttcgt cctttcttct ctaggtcggc cacgggtggg atgggcgcct ggtgtccagg
     1321 tgcaaagagg ggttccacgt ttcccggcga agaacgatcg ctggccgcat cacgcccacc
     1381 tggcgagcgg aaggagaccc acagatgaag atctcatccg attggcatat ccacacgcgc
     1441 aactcgtgcg actcagcatc gctggtcgtc tcggagctga tccccgcggc ggaagcgctc
     1501 aggatcgagg cgtttggcat ctccgaccat atccacacgc cgtacaacct gccggacctg
     1561 gttgccagcc ggcgcgagtt cgacgagagc cagccgccgc cgaacttcca cttcggcgtg
     1621 gaggtgagct gcgtgtcgca gtgggagctg gatgagattg ccaccgggaa gcacgccaac
     1681 ccggtctacg gcctgcggca gggcgggcca gcgtgggggc cgctcgccat cgggatcgga
     1741 gcggaggagc tgaagcgcct cggcgtggag tacgtcatcg ggggcacgca ctggccgatg
     1801 tacctgccgg cggaccgaga cgcgatcatc cgcgactatc atcggcagaa cctcttcctg
     1861 gcgacgcacc cgctggtgac cattgtggcg cacccctggt ggtggatggg ctactggaag
     1921 gacgatgacg gctgctacc
//
LOCUS       DAOSCV010000891         1938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39225_length_1938_cov_2.747212, whole genome shotgun sequence.
ACCESSION   DAOSCV010000891 DAOSCV010000000
VERSION     DAOSCV010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39225_length_1938_cov_2.747212"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLY56_17415"
     CDS             <1..299
                     /locus_tag="PLY56_17415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ23308.1"
                     /translation="KIAWGVHEACLNVIEHAHHFQAAKPLRMRALLYPGRIEFHIFDS
                     GPGFDLSLVPEPDPAQVFERGRGLFGMRQCMDLVEYHQGARVNCLRLVRQIRTA"
     gene            complement(366..>1938)
                     /locus_tag="PLY56_17420"
     CDS             complement(366..>1938)
                     /locus_tag="PLY56_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23309.1"
                     /translation="LREGIRQYYRTHYRLTPEAAASPHLNPGNGWVSRLCFEPRVGVE
                     VLRQMLAPSVEAGKARILLETVPVAAETKGDRIEAIVVRGPDNRLTRIRAAFFLDATE
                     LGDLLPLAGVEHVVGAESREETGEPDAPEEANPEWAQSFTFPFAVELCPGGDFTIPRP
                     EGYEHFRETQPYTFTVDYLPPRGSVTYRMFDTAPGASGPFWTYRRLIDRANFDDPAFP
                     NDIAMINWPGNDYAGGNLANRDAAGRAEMLRQGRLLSLGFLYWLQTEAPRDDGGTGYP
                     ELRLRPDIMGTAHGLSKYPYIREARRIRALKTVAQTEIAADSNPGARAVFFPDSVGIG
                     LYGIDIHACAGNQPQVILGTRPFQIPLGALIPIRVENLLAAAKNIGTTHITNGAYRLH
                     PVEWNIGEAAGALAAYCLSTGTLPHRVATIEEEQRQFQRLLVACGVPLYWYEDVPLSH
                     PAFAAVQWLAATGAWPGHPEHLRFDPDAPLDPDELVNELPREVSARLVTLHEQPGLTR
                     AEFALRLAEEESFLA"
BASE COUNT          314 a          634 c          654 g          336 t
ORIGIN      
        1 ataagatcgc ctggggagtc catgaggcgt gtctgaacgt cattgagcac gcccatcact
       61 tccaggcggc caagcctctg cgaatgcgcg cgcttctcta tccggggcgc atcgagttcc
      121 acatcttcga tagcggccct gggttcgacc tctctctcgt accagaaccg gatcccgcgc
      181 aggtcttcga gcgtggacgc ggcctcttcg gcatgcgcca gtgcatggat ctcgtcgagt
      241 accaccaggg cgcgagagtg aactgcctcc gcttggtccg ccagattcga acggcatagc
      301 cccgcttttt tcccgcgctg gcggatcgta ccctctgttc aatcccctgc gtccggctgg
      361 accgcttacg ccaggaaact ctcctcctcg gccaggcgca gcgcgaactc ggcgcgcgtc
      421 agccccggct gctcgtgcag ggtgaccagc ctcgcgctca cctcccttgg cagctcgttc
      481 accagctcgt ccgggtccag gggcgcgtcc ggatcgaagc gcaggtgctc tgggtgtccg
      541 ggccacgcgc cggtggccgc aagccattgc accgcggcga aagcgggatg cgaaagcggc
      601 acatcctcat accaatagag gggcacgccg caggcgacga gcaggcgctg aaactgccgc
      661 tgctcctctt cgatggtggc cacgcgatgg gggagcgtcc ccgtcgagag acaataggcc
      721 gccagggcgc cggccgcctc gccgatgttc cactccaccg ggtgcaggcg gtaagcgccg
      781 ttggtgatgt gggtggtacc gatgttcttt gcggccgcca gcaggttctc cacgcgaatc
      841 gggatcagtg cgccgagtgg aatctggaag gggcgcgtgc ccagtatcac ctggggctgg
      901 ttcccggcgc aggcatgaat atcgatccca tagaggccaa tgccaacaga gtccgggaag
      961 aaaaccgcac gggcgcccgg gttgctgtcg gcggcgatct ccgtctgcgc cacggtcttc
     1021 agtgcccgga tgcgccgtgc ctcgcgaatg taggggtact tcgagaggcc gtgcgccgtg
     1081 cccatgatgt ccggccggag tctcagctcc gggtagcccg tgcccccgtc atcgcgcggc
     1141 gcctctgtct gtagccagta gaggaaaccg agactcagca accggccctg ccggagcatc
     1201 tcggcgcgtc ccgcggcgtc gcggttcgcc aggttgcccc cagcatagtc gttcccgggc
     1261 cagttgatca tggcgatgtc gttggggaag gcgggatcgt cgaagttcgc gcggtcgatg
     1321 agccggcggt aggtccagaa aggccccgag gcgcctggcg cggtatcgaa catgcgatag
     1381 gtgacagagc cgcgcggtgg caggtagtca acggtgaagg tgtagggctg agtctctcgg
     1441 aagtgctcat agccctcggg ccgggggatg gtgaagtcgc ctcccgggca aagctcgacc
     1501 gcgaagggaa aggtgaagct ctgcgcccac tccgggtttg cctcttcggg ggcatccggc
     1561 tcgcctgtct cctcgcgcga ttcggcgccg acgacgtgct ccacgcccgc cagaggcagg
     1621 agatccccca gctccgtcgc gtctagaaag aacgcggcgc ggatgcgtgt gagccggttg
     1681 tccggaccac gcaccacaat cgcctcgatg cggtcgccct tagtctcggc agcgacggga
     1741 acggtctcga gcaggatgcg cgcttttccg gcctccaccg atggggcgag catctgccgc
     1801 agcacctcca cgccaacgcg gggctcgaag cagaggcgac tcacccagcc gttgcctgga
     1861 ttgaggtgcg gggatgcggc cgcctccggg gtgaggcgat agtgcgtgcg atagtactgg
     1921 cgaattccct cgcgcagc
//
LOCUS       DAOSCV010000892         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39329_length_1935_cov_2.972340, whole genome shotgun sequence.
ACCESSION   DAOSCV010000892 DAOSCV010000000
VERSION     DAOSCV010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39329_length_1935_cov_2.972340"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1935
                     /locus_tag="PLY56_17425"
     CDS             <1..>1935
                     /locus_tag="PLY56_17425"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ23310.1"
                     /translation="EQFGDQMVGMLDDDALAGLREPILAAIDAMRSLGWEPSDLQAWV
                     DEGADGILRQVAALVARQLSPIWEEARRAVLPLRDFRLWGEAEEARSALDEWARAATP
                     IAPDEPLEALVERAEELRALRGVTGKAPRRREYYPREFEELKEAQERIQTLIKTPLVQ
                     HLSLEREIASAEQAVCFLRLALAVWQEYEAAKEAISSLDFNDLQARALKLLETSEGVR
                     RRYQRKFRYLMVDEFQDTDRLQRRVIDLLRGESNFFVVGDVKQSIYGWRNADVRIFME
                     LDRQFSARERLEPDRFRRLSLADNFRSRPEILTFVNSVFADLWVNVPEGTGAVPFEPL
                     ESRGRFEAKEEPSVEVLLVRRGPAAERRQQEADLVARRLKELHDTLPLTSHRRRGERV
                     RWSDCLILLRALTHVSVYEEALVRHGIPYFIVGGRGYYARREIRDLVCLLRVIVSPLD
                     DVATAAALRSPFAGVSLDALAALSRRARELSESPCLYRAIEGVLDDPDLSEEDRSALR
                     RFYALIERLRGISDRMKPGWLLDQAIGETHYDARILAHPDGRRRLANIRKLVQLANAY
                     PAPDVAGLVRYLESLSLVSTDEGDAPSYSEASDVVRIMSVHRAKGLEAPVVFLPDLGR
                     QVRPKAPGLVHIDPEERLIGM"
BASE COUNT          294 a          619 c          680 g          342 t
ORIGIN      
        1 gagcagttcg gcgaccagat ggttgggatg cttgatgatg acgcgcttgc cggcctgcgc
       61 gagccaatcc tggctgccat cgacgcgatg cggtccctgg gctgggaacc ctcggacttg
      121 caggcctggg tggacgaggg cgcggatgga atcctccggc aagtggcggc gctcgtggcc
      181 aggcagctca gcccgatctg ggaagaggcg aggcgggcgg tgctccccct gcgcgatttt
      241 cgcctatggg gcgaggccga ggaagcccgc tccgccttgg atgagtgggc gcgcgctgcc
      301 acgccaattg cgccagatga gccgctcgaa gccctggtcg agcgcgccga ggagctacgt
      361 gctctgcgcg gggtcacggg gaaggcgccc cgtcgccgcg agtattaccc ccgggagttc
      421 gaggagctga aggaggccca ggagaggatc cagacgctca tcaagacgcc cttggtgcag
      481 cacctctccc tggagcgcga gattgcctcc gcggagcagg cggtgtgctt cctgcgcctg
      541 gctctggccg tttggcagga gtatgaggcg gccaaggaag cgatctcctc gctcgacttc
      601 aacgacctgc aggcgcgggc gctgaagctg ctggagacga gcgaaggcgt gcgccgccgt
      661 taccagcgca agtttcgcta cctgatggtg gatgagtttc aggataccga ccgcctgcaa
      721 agacgcgtga tcgacctgct gcggggcgaa agcaacttct tcgtggtcgg cgatgtgaag
      781 cagtcgatct acgggtggcg gaatgcggat gtgcggatct tcatggagct cgaccgacag
      841 ttcagcgccc gggaacggct ggagccggat cgcttccggc ggctctcact cgcggacaac
      901 ttccgctcgc gtccggagat cctaacgttc gtcaactccg ttttcgccga tctctgggtc
      961 aacgtgccgg agggcacggg cgcggtcccc ttcgagccac tggagtcccg gggccgcttc
     1021 gaggcaaagg aggagccgag cgtggaggtg ctcctcgtcc ggcgcggacc cgccgcggag
     1081 cgccggcagc aggaggcgga tctggtggcg agacggctca aggagctgca cgatacgctg
     1141 ccgctcactt cacaccgccg gcgcggcgag cgcgtccgct ggagtgattg cctcatcctc
     1201 ctgcgcgcgc tcactcacgt gagcgtttac gaggaggcgc tcgtccgcca cgggatcccc
     1261 tatttcatcg taggcgggcg gggctactac gctcgccgcg agatccgcga cctggtctgc
     1321 ctcctccgcg tcatcgtcag cccgctggat gatgtggcca cggctgcggc gcttcgctcg
     1381 ccctttgccg gcgtctcgct cgatgcgttg gcggctctct cgcgccgggc aagggagctc
     1441 tcggagtctc cctgtctcta tcgtgccatt gaaggcgtcc tggacgatcc cgatctctcc
     1501 gaggaggacc ggtcggcgct gcgccgcttc tatgctctga ttgagcggct gcgggggatt
     1561 tcggatcgga tgaagcccgg ctggctcctc gaccaggcga ttggcgagac gcactacgac
     1621 gcccggatcc tggcgcaccc ggatggccgg cgccggctag ccaacatccg caagctggtg
     1681 cagctggcca acgcctatcc tgctcccgat gtggcgggcc tggttcgcta cctggagagc
     1741 ctctcgctgg tgagcaccga tgagggcgat gcgccgtcgt actccgaggc gtcggatgtg
     1801 gtgcggatca tgagcgtgca tagagccaag ggcctcgagg ctccggtcgt cttcctcccg
     1861 gacctgggcc ggcaggtccg ccccaaagca cccggcctgg tccatatcga tcccgaggag
     1921 cgcctgatag ggatg
//
LOCUS       DAOSCV010000893         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39471_length_1930_cov_4.291733, whole genome shotgun sequence.
ACCESSION   DAOSCV010000893 DAOSCV010000000
VERSION     DAOSCV010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39471_length_1930_cov_4.291733"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..>1930)
                     /locus_tag="PLY56_17430"
     CDS             complement(239..>1930)
                     /locus_tag="PLY56_17430"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ23311.1"
                     /translation="DSLDDNTLLSFGLPQILLPSVRKIKTRGDFENLMPYLPEEVPEA
                     LFWLAEGDSPEEVRAAMNKPATPAEVDTDDFAKALEHPDSKRRFVVDPTPDELERMLE
                     APLEKWRVFLHPDQERLVMRTFNGPAQVLGGAGTGKTIVAMHRARHLARQIGPDEDGK
                     ILFTTFTANLARNIQQMLQSLCGDEIERIEVIHLHAWAANFLREHGILFTVAEDKEVA
                     ACWDEAISRTGVEEWNPSFIRQEWQAVVQANAIETLQEYLRVPRTGRREKLSRAQRAR
                     LWEVFAAYQDAMAQRNWWEWHDVLREARRYLQENRVALPYRAVIVDEAQDFQEEGWRL
                     IRALVPEGPNDIFLVGDAHQRLYGRKVVLTRLGVRIRGRSSRLRINYRTTEQIRDWAV
                     AMLQGLDVDDLDGGIDTQEGYRSLRSGPPPEVRFFATAREEAAFLKETIEAELQQRKP
                     EEICLVACTTALVENYRLLLETAGIQHVVLSKRMDEGMPGVRVATIHRVKGLEFPCMI
                     VAGANEGVIPSRELSHIEDHEAKERCLLYVAATRARDRLVISSWGTPSPYLPNLT"
BASE COUNT          336 a          633 c          579 g          382 t
ORIGIN      
        1 tgttcctggg acccgcggta acacacaccc ggtttccggg gagccgccat catcggaggc
       61 ggtctcgtgg cctagaggcc cgaggccgcc ttgccagtag acggccatcc ttaggcatat
      121 gggcgcctga ccagcgccgg ctggcattct gatctcggat tcggagagac aaggtcagag
      181 cgtggcgacg cacatcggcc agggtttcgc gttctgcacc cgtccgccct cccggtgctc
      241 acgtgagatt agggagatag ggactgggtg ttccccagga cgagatgacc agccgatccc
      301 gcgcccgcgt ggcggccaca taaagaaggc atctttcctt cgcctcgtga tcctcgatat
      361 gagagagttc tctcgatggg atgaccccct catttgcgcc ggccacaatc atgcagggaa
      421 actccagacc ctttacccgg tggattgtcg cgacccgcac ccctggcatt ccctcatcca
      481 tgcgcttgga gaggaccacg tgttgaatcc ccgccgtttc aaggagcaac cggtagttct
      541 ccaccagagc cgttgtgcat gctactaggc agatctcctc cggcttgcgt tgctggagtt
      601 cggcctcgat ggtttccttc aggaaggccg cttcttcgcg agctgttgcg aagaagcgca
      661 cctctggggg cgggccgctg cgcagagacc ggtagccctc ctgggtatcg attcccccgt
      721 ccagatcatc cacgtccagg ccctgaagca tggcaactgc ccaatcccgg atctgctccg
      781 tggtgcggta gttgatccgc agccggctgg agcgcccccg gatgcgcaca ccaaggcgag
      841 ttagcaccac cttacgcccg tagagccgct ggtgggcatc gccaaccagg aagatgtcgt
      901 ttgggccttc gggcaccagg gcgcggatga gccgccagcc ctcttcctgg aagtcctggg
      961 cctcgtccac gataacggcg cggtagggca gcgccacccg attctcctgc aaatagcgcc
     1021 gtgcctctcg gagcacgtca tgccattccc accagttgcg ctgcgccatc gcatcctggt
     1081 aggcggcgaa cacctcccac agacgcgcgc gctgcgcccg cgagagcttc tcacgccgcc
     1141 cggtgcgtgg aacacgcaga tactcttgca gggtctcgat ggcgttcgcc tgcacgaccg
     1201 cttgccactc ctgtcggata aagctcggat tccactcctc aaccccagtc cgcgagatgg
     1261 cctcgtccca gcaggcggcc acttccttgt cttcggcgac agtgaacagg atgccatgct
     1321 cgcgcaagaa gtttgctgcc caggcatgca ggtggatcac ctcgatccgc tcgatctcgt
     1381 cgccacagag tgattgcagc atctgctgga tgttgcgcgc cagattcgcc gtgaaagtgg
     1441 tgaagaggat cttgccatcc tcatccggcc cgatctgtcg ggcgagatgc cgggcccggt
     1501 gcatcgcgac gatggttttg ccggtgcccg caccccccag cacctgggct ggcccgttga
     1561 acgtgcgcat caccaggcgt tcctgatccg gatggaggaa aacacgccac ttctcaagag
     1621 gcgcttccag catccgctcc agttcgtccg gcgtggggtc gaccacgaag cgccgcttgc
     1681 tgtctggatg ctccagggcc ttggcgaagt cgtcggtatc cacctcggcc ggtgttgccg
     1741 gcttgttcat cgcggctcgc acttcctctg gcgagtcacc ttcagcaagc cagaaaagtg
     1801 cttcgggcac ctcctctggc agatagggca tcaggttttc aaagtcgccc ctggtcttaa
     1861 ttttccgcac cgaggggagc aggatctggg gtagcccgaa agagaggagg gtgttgtcat
     1921 ccagggaatc
//
LOCUS       DAOSCV010000894         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39575_length_1927_cov_2.736111, whole genome shotgun sequence.
ACCESSION   DAOSCV010000894 DAOSCV010000000
VERSION     DAOSCV010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39575_length_1927_cov_2.736111"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>812)
                     /locus_tag="PLY56_17435"
     CDS             complement(<1..>812)
                     /locus_tag="PLY56_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOJ23312.1"
                     /translation="DTAFLEFTIDPDAEPGDREVRIGTPNGLTNAMLFQVGVSPEVYE
                     VQPFGVIKRPTPVLEPPVVINGQCTPGDVDRFRIRARRGQRLVFQVFARHLNPYLADA
                     VPGWFQALIALHDARGQQVAFADDDHFDPDPVLLYEVPEDGEYTLEIRDALYRGREDF
                     VYRVQVSEQPFITRIFPLGGREGTDVNVLLGGWNLAAKAARLDTRPGHDEIRRLALPA
                     GTGARHHLAYAVNTLPEVFEEEPNDTRKEARRVTPPVIINGTISRPGDVDCY"
     assembly_gap    813..822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<823..1317)
                     /locus_tag="PLY56_17440"
     CDS             complement(<823..1317)
                     /locus_tag="PLY56_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23313.1"
                     /translation="MGSLLPRRGLDAGWAKLGLRTGLVVGFLVALAVPAVAGPGFREP
                     HIAYLFPAGWQRGNVIRVWCGGQYLGGMDRVHVSGKGVRGRVLFYAPPLNSEDFDELR
                     RRRAARGNQPATALQASAGQGTEGKKWNPLIDLLDELNPLELQTVLGDFFSPNTKRQP
                     NAQIS"
     gene            1684..>1927
                     /locus_tag="PLY56_17445"
     CDS             1684..>1927
                     /locus_tag="PLY56_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23314.1"
                     /translation="MMPIAGLTTLLLLAAPMPQPGVRHPVLPVQVSAQAAEGYRAELA
                     PVLAMEEKRLVSLVPTQSGLYFVGCPNCNEGRQEGQF"
BASE COUNT          305 a          670 c          635 g          307 t
ORIGIN      
        1 ggtagcagtc cacgtcgccc ggccggctga tcgtgccgtt gatgatcact ggtggcgtca
       61 cgcggcgcgc ctctttgcgc gtgtcgttgg gctcttcctc gaacacctcg ggcagcgtgt
      121 tcacggcata ggcgaggtgg tggcgcgcgc cggtgcctgc cggcaaggcg agccgccgaa
      181 tctcgtcgtg cccgggccgc gtatccaggc gcgccgcctt cgccgcgagg ttccagcccc
      241 cgagcagtac attcacatcc gtgccctccc gccccccgag cgggaagatg cgcgtgataa
      301 agggctgctc cgagacctgc acgcggtaga cgaaatcctc gcgcccgcgg tagagggcat
      361 cgcggatctc aagcgtgtac tccccgtcct cgggcacctc gtagaggagc accgggtcgg
      421 ggtcaaagtg gtcatcatcc gcaaacgcca cctgctggcc ccgggcatcg tgaagggcaa
      481 tcagtgcctg gaaccagccg ggcacggcat ccgccaggta gggattgaga tgccgggcga
      541 acacctggaa gacgaggcgc tgcccccggc gggcgcgaat gcgaaagcga tccacgtcgc
      601 caggcgtgca ttggccgttg atcaccacgg gcggctccag caccggcgtg ggccgcttga
      661 tcaccccaaa cggctgcacc tcgtacacct cgggcgagac gcccacctgg aagagcatgg
      721 cgttggtcag gccgttgggc gtgccgatgc gcacctcgcg gtcgcccggc tccgcatcgg
      781 ggtcgatcgt gaactcgagg aacgccgtat ccnnnnnnnn nncgaaatct gcgcgttggg
      841 ctggcgcttg gtgttggggc tgaagaaatc accgagcacc gtctgcagtt ccagagggtt
      901 gagctcatcg aggagatcga tcagggggtt ccacttcttc ccctcagtgc cttgaccggc
      961 gctcgcttgc agggcagtgg caggctgatt cccccgcgcg gcgcgccgcc tgcgcagctc
     1021 gtcgaaatcc tcgctgttga ggggcggagc gtagaacaga accctcccgc gaacgccttt
     1081 cccggagaca tgcacgcgat ccatgccgcc caggtattgc ccgccgcacc agacgcggat
     1141 cacattcccc cgctgccagc cggcgggaaa gagataagcg atgtgcggct cgcgaaagcc
     1201 cggcccggcg actgccggca cggcgagcgc aaccaggaac cccacgacga gtccggttct
     1261 caggcccagt ttcgcccatc ccgcgtccag ccctctgcgc ggcagcagcg atcccatggc
     1321 tgcattcctc ccaggggcgg caggcgctgc ccccgcaacg cgtacccgag cggttatctg
     1381 catccgggcc gtggcccgtg gcggcgcact ggcgccatcg gcccccccct ggcagcgttc
     1441 gtctgcggaa cgccgccagc cgatgccctc tttctacgcg ctcccgtctc actcctgcct
     1501 cacctgcact gctgaccaca gcgggcaagc gcccggcgcc tccgacgccg atcaccggca
     1561 gcgcggtttg gccatcccta ctcgtcgggc ggagaaacgg gacccggtgt cgaaaggaga
     1621 gggcaacggc accgcgccct catgcagggc ctgggttgcg cgcacgtttg ggaagagagg
     1681 cgaatgatgc cgattgcagg actgaccacc ttgttgcttc tggcggctcc gatgccgcag
     1741 ccgggcgtcc ggcacccggt tctgcccgtt caggtgagcg cgcaggcggc cgagggatac
     1801 cgcgccgaac tcgcgcccgt cctcgcgatg gaggagaagc gtctggtctc cctggtgccc
     1861 acccaatcgg gtctctactt cgtcggctgt ccgaactgca acgagggtcg gcaggagggc
     1921 cagtttg
//
LOCUS       DAOSCV010000895         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39613_length_1926_cov_2.533939, whole genome shotgun sequence.
ACCESSION   DAOSCV010000895 DAOSCV010000000
VERSION     DAOSCV010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39613_length_1926_cov_2.533939"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLY56_17450"
     CDS             <1..300
                     /locus_tag="PLY56_17450"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ23315.1"
                     /translation="EAFILAVLGRDRPGIIARVTGYLAEQGINIEEFTANVVEGNLVL
                     ILSVRVPASLDIAGVQSAIEAVGQEFGITASLQHENVFRATSEVHAVARLRSARR"
     gene            346..1716
                     /locus_tag="PLY56_17455"
     CDS             346..1716
                     /locus_tag="PLY56_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012624258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HOJ23316.1"
                     /translation="MLSSEDILTTVSMLQNEHLDVRAVTLGIDLFSCADRDPETLCRL
                     AREKILRHAGGIQEACQRAVERYGIPIVNRRIALSPVAPLVAEHEPETLLRFARMLDA
                     VAEEAGVDFIGGWTAHVQKGMTLASRALIDSLPDVLSETTHLCASVNAASSHAGINMD
                     ALLLLGRKIREMAERTADRDGFACCKLVIFANQPEDNPFMAGAYKGLGEPECVVNIGV
                     SGPGVVKRAIERLRQSGEPLTLGDIAEEIKLTAFRVTRVGELIGREVAASLGAEFGIV
                     DLSLAPTPSVGDSVGEILEAMGVRHVGGPGSTAALALLNDAVKKGGAFASSSVGGLSG
                     AFIPVAEDAALAAAAEAGHLRVEKLEAMTAVCSVGLDMVVLPGDTTAETLAALAADEL
                     AIGVVNRKTTAVRLVPVPGKRAGDRAVFGGLFGESPILPIPTGASDDFVRLGGRIPAP
                     LVSLNN"
     gene            complement(1727..>1926)
                     /locus_tag="PLY56_17460"
     CDS             complement(1727..>1926)
                     /locus_tag="PLY56_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HOJ23317.1"
                     /translation="AGPLVKLDLVAEWGDPVHVELPRERYHELALAPEMVVCVRPKEP
                     GALLLQPPDEEHNAPAEMYYI"
BASE COUNT          286 a          637 c          687 g          316 t
ORIGIN      
        1 gaggcgttca tcctggccgt cctggggcgc gaccgcccgg ggatcatcgc acgcgtcacc
       61 ggctacctgg cggagcaggg gatcaacatc gaggagttca cggccaacgt cgtcgagggc
      121 aacttggtgc tcatcctgag cgttcgcgtg cccgcctcgc tcgacatcgc cggggtgcag
      181 agcgccatcg aggcggtggg gcaggagttt gggattacgg ccagcctgca acatgaaaac
      241 gtctttcgcg cgacgagcga agtgcatgcc gtcgcgcggc tgcgctccgc ccggcggtaa
      301 agggccaggg gcccgctgcc ctcaaccatg ctctaaggga aagagatgct ctcatccgag
      361 gatatcctga cgactgtttc catgctccag aacgagcacc tcgacgtgcg tgccgtcacc
      421 ctggggatcg atctcttctc gtgcgccgac cgagacccgg agacgctctg ccgcctggcg
      481 cgggagaaga tcctgcgcca cgccgggggc atccaggagg cgtgccagcg cgccgtggag
      541 cgctatggca tcccgatcgt caaccggcgc atcgcgctca gtccggtggc gccacttgtg
      601 gcggagcatg agccagaaac gctgctgcga ttcgcgcgca tgctcgacgc ggttgccgag
      661 gaagcaggcg tcgatttcat cggtggctgg acggcccacg tgcaaaaggg gatgacgctc
      721 gccagccggg cgctgatcga ttcccttccc gacgtcctct cggagacgac ccacctctgc
      781 gcgtcggtga acgctgcctc gtcgcacgcc gggatcaata tggatgccct cttactcctg
      841 gggcgcaaga tccgcgagat ggccgagcgc accgccgacc gcgacggctt tgcctgctgc
      901 aagctggtca tctttgccaa ccagccggag gacaacccct tcatggcggg tgcctacaag
      961 ggcctgggcg agccggagtg cgtggtgaac atcggtgtca gcggcccggg cgtggtgaag
     1021 cgcgccatcg agcgcctgcg ccagtcgggc gagccgctca ccctggggga tatcgccgag
     1081 gagatcaagc tgaccgcatt tcgggtgacc cgcgtgggtg agctaatcgg gcgcgaggtg
     1141 gccgcctccc tgggcgccga gtttgggatc gtggacctct cgctggcgcc gacgccctcg
     1201 gtgggcgaca gcgtgggcga gatcctcgag gcgatgggtg tgcgccacgt tggcggcccg
     1261 ggctcgaccg cggcgctggc gctcctcaac gatgctgtga agaagggagg cgccttcgcc
     1321 tcgtcgtcgg tgggcggtct ctccggggcg ttcatcccgg tggcggagga tgctgctctg
     1381 gcggccgccg cggaggccgg ccacctgcgc gtggagaagc tggaggcgat gaccgccgtc
     1441 tgctccgtgg gcctcgatat ggtggtgctc cccggcgaca ccacggcgga gaccctcgct
     1501 gcgctggccg ccgacgagct ggcgatcggc gtcgtcaacc ggaagacaac ggcggtgcgc
     1561 ctggtgccgg tgccgggcaa gcgcgcgggc gaccgcgccg tctttggcgg gctgttcggc
     1621 gagagcccga tcctcccgat cccgacgggc gcctccgatg acttcgttcg cctgggcggg
     1681 cggattcccg cgcccctggt cagcctcaac aactgacccg ctcgcttcag atgtagtaca
     1741 tctcggctgg ggcgttgtgc tcctcgtcgg gcggctggag aagtagcgcg cctggttcct
     1801 tgggacgcac gcagacgacc atctccggcg cgagcgcgag ctcgtggtag cgctcgcggg
     1861 gaagctccac gtgcaccgga tcgccccact ccgcgacgag atcgagcttt accagggggc
     1921 ccgcgg
//
LOCUS       DAOSCV010000896         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39669_length_1924_cov_3.120920, whole genome shotgun sequence.
ACCESSION   DAOSCV010000896 DAOSCV010000000
VERSION     DAOSCV010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39669_length_1924_cov_3.120920"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="PLY56_17465"
     CDS             complement(<1..1704)
                     /locus_tag="PLY56_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ23318.1"
                     /translation="MAVARELHSRLEARDRAARYAEALARLASAASAREDPISILQLL
                     VQEAADAVGLAEAHVLIAPSVLFPDGLHVNTASSLSGPFDPDAQDAPSRLLAQILAGK
                     DRELALEAGDEFDGWVGFPISDLRKPVGILAVRTRPSSAADAPPLPPEARELLARMAG
                     QAGLVIENSALFGDVVRRAWEVAGIADLAGAIVTEADLPRLLDFAAQRAATITEAATS
                     AILLADRHRRSWRVEVAHGAHHASLQGIQAPIDFGLPGRVIHDGRMRFAAAPELVMEI
                     VPDTPLEDVGAALFVPLSRRDSTSGKGAMPGDPDAPALGCLVCLMPQGTSSFSGKDVR
                     VLTIIADQVAMAVENACLYRRAVQSEQLQDGVLKLVEKLRRETTSAGVLAHGCRCAAE
                     LLEMHAVVACQYPEGAVPPRPVQIHAPWEGAPSACSCNLGEDPSRRACPAASVLRDPS
                     ARPLRSPDDLRQCPLLRDLGAKSWAGVPLHLQGGPRTLVLFIDREDPDRFDDEMLQRA
                     ELVCTYLGSALEMACVAEAQRQEVEVSNALLSIAHLIATERDPGRILRTLNEVTLNCL
                     SH"
BASE COUNT          346 a          636 c          660 g          282 t
ORIGIN      
        1 gtggctcagg cagttgagcg tcacttcatt gagggtgcgg aggatgcggc ctgggtcgcg
       61 ctcggtggca atcaggtggg cgatggagag gagcgcgttg gagacctcca cctcttgccg
      121 ctgcgcttcg gcgacgcagg ccatttcaag cgcgcttccc aggtaggtgc acacgagctc
      181 ggcacgctgg agcatctcgt catcaaaacg gtcgggatcc tcccggtcaa tgaagagcac
      241 cagcgttcga ggccccccct gcagatgcag cggcacgcct gcccaggact tcgcgcctag
      301 atcccgcagg agcgggcact gccgcaaatc gtccggcgag cggagcgggc gcgcgctcgg
      361 gtcacggagc acgcttgcag ctgggcaggc acgtcgggat gggtcctcac cgaggttaca
      421 ggagcaggcc gagggggccc cttcccacgg cgcgtggatc tgcaccggcc gcggcggaac
      481 agcaccttct ggatactggc acgctacgac ggcatgcatc tccagcagct ccgcggcgca
      541 gcggcacccg tgcgccagta caccggcgct cgtcgtctcg cggcgcagct tctccaccag
      601 cttgagcacc ccatcctgga gctgctcgct ctggacagcc cggcgataga ggcacgcgtt
      661 ctcaaccgcc atggccacct ggtccgcgat gatcgtcagg acgcgcacat ccttcccgga
      721 gaaggaagac gtgccttggg gcatcaggca gacgaggcag cccaaggcgg gagcatcggg
      781 atcgccaggc atggcgcctt tccccgaggt ggaatcacgc cggctgaggg gcacgaagag
      841 cgccgcgccg acatcctcca ggggggtgtc cggcacgatc tccatgacca gctccggtgc
      901 ggcggcgaag cgcatgcggc catcgtggat cacccggccg ggcagcccga aatcgatcgg
      961 cgcctggatt ccctgaagag aggcatgatg ggcgccgtgc gccacctcca ctcgccatga
     1021 gcgtcggtgc ctgtccgcca ggaggatcgc gcttgtagcg gcctcggtga tagtggcagc
     1081 gcgctgcgcg gcgaagtcga ggagacgggg caggtcggcc tcagtcacga ttgcgccggc
     1141 cagatctgcg atgcctgcca cctcccaggc acgccggacc acgtctccaa agagggcgct
     1201 gttttcgatc acgaggcccg cctgtccggc catgcgcgcc agcagttcgc gcgcctctgg
     1261 gggcaacggt ggcgcgtccg cggcggatga agggcgcgtg cgaacagcca gaatgcccac
     1321 cggcttgcgc aggtcggaga tcgggaagcc gacccacccg tcgaactcgt cgcctgcctc
     1381 cagtgccaat tcccggtcct tccctgccag gatctgcgcg aggagccgcg aaggggcatc
     1441 ctgggcgtct ggatcaaagg ggccagagag cgaagaggcg gtgttcacgt gcaggccgtc
     1501 cgggaagagg acgctgggag caatgaggac gtgagcctcg gcaaggccga cagcatcggc
     1561 cgcttcttgg acgaggagct gaaggatgct gatcggatct tcccgcgcac tcgctgccga
     1621 ggcgagtcgg gcaagcgctt cggcgtagcg agctgcgcgg tcccgcgcct ctaaccgcga
     1681 gtggagctcc cgcgcgactg ccacatggga acgcctggag agccaccagg cgaacgctgc
     1741 gccactcgtg agaccgatac caaccgcacg aaccaccagg ggtgccggcc acaacacaga
     1801 tccaacgagc acagtggcga gcgaacctac gcaggcccat gatacagcct ggtgacggag
     1861 tttggctgga acaagcacaa gctctccggt ccgatgctcc ccaaagggat atttgttctg
     1921 gccc
//
LOCUS       DAOSCV010000897         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39692_length_1923_cov_3.437366, whole genome shotgun sequence.
ACCESSION   DAOSCV010000897 DAOSCV010000000
VERSION     DAOSCV010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39692_length_1923_cov_3.437366"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PLY56_17470"
     CDS             complement(<1..1327)
                     /locus_tag="PLY56_17470"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ23319.1"
                     /translation="MPTPNMTRVAEAASPGTGTSLLEDLTRDQLENGMKLLQQKKFKE
                     AAAEFKALTQSAPEEPAPWFYLGMAQAQAGQLAEAAQALKTAGKLQPENKEIWTNLAA
                     VYQGMKRTRDAVQAIERAAKIDPKDAGLQALLAQGYMQLGDAARARAAAECAAKLDPN
                     IAAVLQAQGAGPGGAPDLKALREAVRKHPNDVRARAQLAQVLLMTGDIAGARDAFERV
                     AAAKPGDPEAHRALARIYLAQGKHAEAARSLGRLVALEPDDLDARWALANLLHEAKQP
                     EKAAAQLVAILKVKPRHVPANMALGTIRYQQKRFADAEKCFRTAFTAEPANLEAARSL
                     TMLLRERGRAGDAEKVLRRALRASPKQPLLHAMLGDLLESQSRPKEAIAAYRQAQALA
                     PKEPEIALALGHLLETQKKEAEAVALYTAFLKKAPDATPVRDSLAALHAR"
     gene            complement(1441..1701)
                     /locus_tag="PLY56_17475"
     CDS             complement(1441..1701)
                     /locus_tag="PLY56_17475"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23320.1"
                     /translation="MLTPICPHGLTLRPLVLPGDSTVELRVENTADTEVVVTVDGQTG
                     VPLAPEERVVVSQAPFRARLITPDTGDFFRKLREKLGWGERC"
     gene            complement(1701..>1923)
                     /locus_tag="PLY56_17480"
     CDS             complement(1701..>1923)
                     /locus_tag="PLY56_17480"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23321.1"
                     /translation="IEGPERAPQRFIALNDAVVAKGTLARLLHLRAFVDGRYVTAYAA
                     DGVIVSTPTGSTATPSLPGGRWRTRRSRC"
BASE COUNT          277 a          684 c          636 g          326 t
ORIGIN      
        1 gacgcgcgtg gagcgccgcc agggaatcgc gcacgggggt cgcatccggc gccttcttca
       61 agaacgcggt gtagagcgcg acggcctcgg cctccttttt ctgcgtctcc aacaggtggc
      121 ccaaagcgag ggctatctcc ggctctttgg gggcgagtgc ctgtgcctgg cgataggcgg
      181 cgatcgcctc cttggggcgc gactggctct ccaggaggtc gcccagcatc gcgtgaagca
      241 gcggctgctt cggcgaggca cggagcgcac gccggagcac cttctcggcg tcgcctgcac
      301 gcccgcgctc gcgcaggagc atggtgaggc tcctggcagc ctcgaggttg gccggctccg
      361 ccgtgaacgc ggtgcggaag cacttctccg cgtctgcaaa acgcttctgc tggtagcgga
      421 tcgtccccag cgccatgttg gctggcacgt gccggggctt cactttcagg atggcgacca
      481 gctgggccgc ggccttctcc ggctgcttcg cctcgtgcag caggttcgcg agggcccagc
      541 gggcatccag gtcatccggc tcaagcgcca cgagacgccc gagagagcgc gctgcctcgg
      601 catgcttgcc ctgggccagg tagatgcgcg ccagcgcccg atgcgcctct ggatcccccg
      661 gcttcgcggc ggcaacacgc tcgaacgcat cccgggcgcc cgcaatatcg cccgtcatca
      721 gtagcacctg ggcaagttgc gcgcgcgccc gaacatcgtt ggggtgcttg cgcaccgcct
      781 cgcgtagcgc cttcagatcc ggagcgcccc cgggcccggc accctgtgcc tgaagcacgg
      841 cggcgatgtt cgggtccagc ttcgcggcac actccgcggc agcccgagcg cgcgcggcat
      901 ctccgagttg catgtaaccc tgggccagga gcgcctgtag cccggcgtcc ttggggtcga
      961 tcttggccgc gcgctcaatt gcctgcacag cgtcgcgggt acgcttcatc ccctggtaga
     1021 cagccgccag gttcgtccag atctccttgt tctccggttg aagctttccc gcggtcttga
     1081 gcgcctgcgc ggcctcggca agctggcctg cctgggcctg agccatcccg agatagaacc
     1141 acggcgcggg ctcttcgggc gccgattgtg tcagcgcctt gaactcggcg gcggcctcct
     1201 tgaacttctt ctgctggagg agcttcatgc cgttctcgag ctgatcgcgc gtcagatcct
     1261 ccaggaggct ggtgcccgtg cccggactgg ccgcttcggc gacgcgcgtc atgttcggtg
     1321 tcggcacggg cggcttctcc gcggcgccag gcttcctgca gcccgccagc cagagcgatc
     1381 cgcccccgat accggtggcc aaagccaccg ccagcgcacc tgtccgagtc cacctcagat
     1441 tcaacagcgt tccccccacc ccagcttctc ccgcagcttg cggaagaaat ctcccgtgtc
     1501 cggcgtaatc agccgggcac ggaaaggtgc ctggctcacg accacgcgct cctcgggcgc
     1561 cagcggcact cccgtctggc catccacggt caccacaacc tccgtatccg ccgtattctc
     1621 caccctgagc tcgacggtcg aatcgcccgg caacaccagc gggcggagcg tgagcccgtg
     1681 aggacagatc ggggtgagca tcagcacctc gacctccggg tgcgccagcg gccccccggc
     1741 agagagggag tagcagtcga gccggttggc gtcgagacga tgacgccatc ggcggcgtac
     1801 gcagtgacgt agcggccatc cacgaaggcg cgaaggtgca ggagccgggc cagagtgccc
     1861 tttgctacca cggcgtcgtt gagggcgatg aacctttggg gcgcccgctc tgggccttcg
     1921 atg
//
LOCUS       DAOSCV010000898         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39695_length_1923_cov_3.047109, whole genome shotgun sequence.
ACCESSION   DAOSCV010000898 DAOSCV010000000
VERSION     DAOSCV010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39695_length_1923_cov_3.047109"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="PLY56_17485"
     CDS             complement(<1..899)
                     /locus_tag="PLY56_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOJ23322.1"
                     /translation="MKAVVMAGGEGSRLRPLTCNRPKPLVPIVNKPIMQHIIELLVRC
                     GFTDVIATLHYLADEIETYFGDGSEFGVRMRYTVEETPLGTAGSVKQAEPWLSDGTFI
                     IISGDALTDFDLRKAVEFHKRQGAMATIVLTRVESPLEYGVVITDQDSRIRRFLEKPD
                     WSQVFSDTINTGIYILEPEVLSYMELGKNYDWSQDIFPQLLKERKPLLGYLASGYWCD
                     VGSLQAYRQAHYDALRGKVRLNIPGERGEDEIWVHPTARIEPGVRINGPAVIGSHATI
                     RRGAVIEEFTVLGDSCIVEREAEV"
BASE COUNT          356 a          612 c          606 g          349 t
ORIGIN      
        1 acctctgcct cgcgctcgac gatgcacgag tcgccgagta cggtgaactc ctcgatcacg
       61 gcgccgcgtc ggatcgtcgc atggctgccg atcaccgccg gaccattgat gcgcacgccg
      121 ggctcaatcc gcgcggtggg gtgcacccaa atctcatcct caccacgctc tcccggaatg
      181 ttgaggcgca ccttgccgcg cagggcgtcg tagtgggcct ggcggtaggc ctggaggctg
      241 cccacgtcgc accagtagcc agaggcgagg tagccgagga gcggcttgcg ctccttgagg
      301 agctgcggaa agatatcctg gctccaatcg tagttctttc cgagctccat gtaggagagc
      361 acctccggct ccaggatgta gatgccggtg ttgatggtgt cgctgaagac ctggctccag
      421 tccggcttct ccaggaagcg gcggattcgg gagtcctggt cggtgatcac cacgccatat
      481 tcgagcgggc tttcgacgcg cgtgaggacg atggtggcca tcgcgccctg gcgcttgtgg
      541 aactcgaccg ccttgcgcag atcgaagtcg gtgagcgcgt caccggagat gatgataaag
      601 gtaccgtcgg acagccacgg ctcggcctgc ttcacgctgc ccgcggtgcc caggggcgtc
      661 tcctcgaccg tgtagcgcat ccgaacacca aactcggacc catctccaaa ataggtctca
      721 atctcatcgg ccaggtagtg cagcgtggcg atgacgtccg tgaagccaca ccggaccagc
      781 agctcgatga tgtgctgcat gatcggcttg ttgacgatgg gtacaagagg cttgggacgg
      841 ttgcaggtga gaggacgcag acgacttccc tcaccgccgg ccatgacgac cgccttcatt
      901 cttcgctcct tcccccacca agcgggtgag tggcgcgaga aggcaagtcc tgttccggcc
      961 ggttttggat ccagccgccg gctggctcgg cgtggcaacc tgccggaacc cgggctaaag
     1021 cctgctcgtc cgaacaccca ctcagtgcgc ggtcgcgctg gcgcgccggc cggctagagc
     1081 cctggagagg gcattcaccg tgtctggcac gcccgcgaga tgcaccaagc attccacgtg
     1141 cgagatccga ggcggcgccc ggggccccac ggggcggggc tttgccgggc gtgtcggcga
     1201 ccgatcagtt gtgtatggaa ttccgttcgc gagcgacctc gagcggcgct gcttccgatg
     1261 accagggcct tccttgccgc cgggtggatg gccggcaggc acccgcgcgc tgcaacggct
     1321 gcttccggaa gcgcgcacac ggggcaaagg aaggccgctc gttacgtgaa gagcccggct
     1381 cttactccaa aaggctgctt cgcgagcggc agttggtatc tgctccttat tataccagat
     1441 aacaagagga tgtaaagaag caagcagggg ttactggcat tccgagatga ggaatccgat
     1501 tcagagcaca gagaccctcg gctccaatgc ccggacctct ccggcatcct ctaccatagc
     1561 cccccagggt ggccctgccc cggccctgaa gggccgcata acctagccca gggcatccgc
     1621 accctgggtc atgcaaaggt aaaaataacc acgacctcaa agaggttgca caaaccgcta
     1681 ccacatattg tcgaaccctt tcagggttcc aggtattttc tgaacacgag acccagggtc
     1741 aggagaccct gggttgtact gcgggaccgg gttggggtcc ttcgagagcg tcacccgggt
     1801 ggagatacca ccgattccgg atgaatcgca tcccctgggc gcctacccgg cccatcggtg
     1861 ttcgtcgcct tcattcgtgg tatcggcgcc accgcgccaa tcacccggcg ggtgaaggtt
     1921 gca
//
LOCUS       DAOSCV010000899         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39730_length_1922_cov_3.407606, whole genome shotgun sequence.
ACCESSION   DAOSCV010000899 DAOSCV010000000
VERSION     DAOSCV010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39730_length_1922_cov_3.407606"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PLY56_17490"
     CDS             <1..401
                     /locus_tag="PLY56_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOJ23323.1"
                     /translation="EYEAAAGSYSIKRAMLDKAKKDALIMHPLPRVDELDYRIDRDRR
                     AAYFRQAGNGVPIRMALAALLLGAERTPDGTRPPEARSATVRTPPDLVCPNERCVTRN
                     EAYLAPRFVSIVGHEEVLQCAYCDGEVPRP"
     gene            448..1539
                     /gene="carA"
                     /locus_tag="PLY56_17495"
     CDS             448..1539
                     /gene="carA"
                     /locus_tag="PLY56_17495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOJ23324.1"
                     /translation="MVKASLVLEDGTVWTGTAIGAAGRGYGEVVFNTSMTGYQEMLTD
                     PSYAGQILTLTYPLIGNYGATEPDTESRRVQVSGLIVRELCETPSNWRSQGTLQEYLE
                     RNNVVGIQGIDTRALTRHLRVRGVMMGALSTGEDPEAMLAWLKALPDYTKIDFARRVT
                     TPEPYTWEEPADAAAPEVRHHVVAIDCGLKYSILRSLRSLGCRVTVVPCTTTPAEILA
                     HRPDGILLSPGPGNPALLGYLVDTIRALLGKLPILGICLGNQLVGCALGGSTFKLKFG
                     HRGGNHPVKDLTTGRVYITSQNHGFALDPESLAGTGAEVTHVNLNDGTVEGIRHEGKG
                     VFTVQYHPEASPGPWDNFYLFEQFVERMG"
BASE COUNT          329 a          630 c          650 g          313 t
ORIGIN      
        1 ccgagtatga agcggctgcc ggcagctact ccatcaagag ggcgatgctc gacaaggcga
       61 agaaggacgc cctcatcatg cacccgctcc cccgggtgga tgagctggac tatcgcattg
      121 atcgtgaccg ccgggccgcc tacttccgcc aggcaggcaa cggcgtgccg atccgcatgg
      181 cgctcgcggc attgctcttg ggtgccgagc gcacccccga cggcacgcgt cccccggagg
      241 cgcgctccgc cacggtgcgc accccgccag acctcgtctg ccccaatgag cgctgcgtta
      301 cccggaacga ggcctatttg gccccgcgct tcgtgagtat cgtcggccac gaggaggtcc
      361 tccagtgcgc ctactgcgac ggggaggtac cgcggccctg atgtgcatgg gaatgtggga
      421 gcatggaagc atgggaatag aggtggcatg gtgaaggcat cgcttgtgct tgaagacggg
      481 accgtgtgga caggaacggc catcggcgcc gccggacgcg gctacggcga ggtggtcttc
      541 aacaccagca tgacgggcta ccaggagatg ctcaccgacc cctcctacgc tgggcagatc
      601 ctcaccctca cctacccgct gatcggcaac tacggcgcca ccgagccgga caccgagtcg
      661 cgccgggtgc aggtctccgg gttaatcgtg cgcgagctgt gcgaaacgcc gagcaactgg
      721 cgctcgcagg gcacgctgca ggagtatctg gagcggaata acgtcgtcgg cattcagggg
      781 atcgatacgc gcgcgctcac ccgccacctg cgcgtgcgcg gcgtgatgat gggcgccctc
      841 tctaccggcg aggatcccga ggcgatgctc gcctggctga aggccttacc cgactacact
      901 aagatcgatt tcgcgcgcag agtgaccacc ccggagccct acacctggga ggagccggcg
      961 gatgcggccg cccccgaggt gcgccaccac gtcgttgcca tcgattgcgg gctgaagtac
     1021 agcatcctcc gctcgctgcg ctcccttggc tgccgggtca cggtggtgcc ctgcacgacg
     1081 accccggcgg agatcctggc gcaccgaccc gatggcatcc tcctctcgcc cggccccggc
     1141 aacccggcgc tccttggcta cctggtcgat accatccgcg cactccttgg caagctgccg
     1201 atactcggca tctgcctggg caaccagctc gtcggctgcg ccctgggcgg ctccaccttc
     1261 aagctgaagt tcgggcaccg cggcggcaac cacccggtga aggatctgac cacgggacgg
     1321 gtctacatca cctcgcagaa ccacggcttc gccctcgacc cggagagcct ggccggcact
     1381 ggcgccgagg tgacgcacgt gaacctgaat gatggcacgg tcgagggcat ccgccacgag
     1441 gggaagggcg tcttcaccgt gcaataccac ccggaggcaa gccccggccc ctgggacaac
     1501 ttctatctct tcgagcagtt cgtggagcgg atgggctagc ggtgtgtctg gtgcggttgt
     1561 gtagggcgat gctggctatc gccctggcct gttacccgcc gggatgccgg cggttggaga
     1621 ccgcgggcga tacctacccg aagtcggccg gcgccggctg gagagcccgc gaaggcaggc
     1681 ttcgtaccgt tgttgcccgc gacctcggtc gccgggtgcc ctggagtgtc ggcatgtgac
     1741 atcttgagaa ctgcgggttt ctcgagagtg agacagatac ggacgaggtg gatggatgcc
     1801 taagaaggca gagattaaga aggtgatggt catcggctcg ggtccgattg tgatcgggca
     1861 ggcggcagag ttcgactatg ccggaacgca ggcgtgccgg gcgctccgcg aggagggcgt
     1921 cg
//
LOCUS       DAOSCV010000900         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39732_length_1922_cov_3.098554, whole genome shotgun sequence.
ACCESSION   DAOSCV010000900 DAOSCV010000000
VERSION     DAOSCV010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39732_length_1922_cov_3.098554"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLY56_17500"
     CDS             <1..1234
                     /locus_tag="PLY56_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23325.1"
                     /translation="HRVAVIAPLELALENHGFDPEAGTARLRATVRNHRAQSARVELA
                     AQAPSGWKVTGAGPLSVPASAERSVELRLAPEGASAAGMVDVNVTATAGADRCSARAF
                     LLHIPKEANLLKNAGFEEGNTGWSARAEQIDQTVARSGKASLKLENRVVGQTQVGQSV
                     TLNQKAPCPILVQVSSRALNVSGEKGKGYALYVDIYYTDGTPLYGQTFDFQTGTTEWQ
                     FGQVYIEPAKPVRVVNVYLLLRGKSGTVWFDDVAVMEDLRRKGNVAREAQVTVDSSFS
                     GYDSSPVNDGVTVGEGLHWTKEAWASAEEARPHFIELRFKEPRSIARALVYWSLDGGV
                     ARTSREIAVEIPEGDGWKRVALVKPGAPEPVTEIRFAQPVQSERFRFVQPMGAGPQGR
                     PNLMWVREVELFPESTAR"
     gene            1416..>1922
                     /locus_tag="PLY56_17505"
     CDS             1416..>1922
                     /locus_tag="PLY56_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23326.1"
                     /translation="MSYRISWFLAACLAILCGAPLVAAPAEVPLKNPSFEEGVDQRGT
                     PLGWQRYGGGGQGQRLSVVDVPGGGKALLIEDGDPNAEIGITQTAPARPEEVYRASVR
                     VGRVAGADAAGAYLQLRFLPSQKFVQVALMPGEGMAFRAVSATMQAPAGTTGLMLYLY
                     THKAPTPKV"
BASE COUNT          309 a          598 c          701 g          314 t
ORIGIN      
        1 ccacagggtg gccgtcatcg cgccgctgga gctggcgctc gaaaaccacg gcttcgaccc
       61 ggaggccggc acggcgcgcc tgcgggccac ggtgcgcaac caccgggcgc agagcgcgcg
      121 cgttgagctg gcggcgcagg cgccgtccgg ctggaaggtg accggggccg ggccactttc
      181 ggttcccgcc tcggcggagc gctcggttga gctgcgcctg gcgccagagg gcgcgagcgc
      241 cgccgggatg gtggacgtga acgttaccgc gacggctggc gccgaccggt gcagcgcgcg
      301 cgccttcctc ctccacatcc ccaaggaagc gaacctgctc aaaaatgccg ggttcgagga
      361 ggggaacacc ggctggtcgg ccagagcgga gcagatcgac cagacagtgg cgcgaagcgg
      421 caaggcgtcg ctgaaactgg agaaccgcgt cgttggccag acccaggtgg gccagtcggt
      481 gacgctcaat cagaaggcac catgccccat cctggtgcag gtgtcgtcgc gcgcgctcaa
      541 cgtgagcggc gagaagggca agggctacgc gctctacgtc gatatctact acaccgatgg
      601 caccccgctc tacgggcaga cgttcgactt ccagacgggc accacggagt ggcagttcgg
      661 ccaggtgtac atcgaaccgg ccaagccggt ccgggtggtc aacgtttacc tgctcctccg
      721 gggcaagagc ggcaccgtat ggttcgatga cgttgcggtg atggaggacc tacggcgcaa
      781 ggggaacgtg gcgcgcgaag cccaggtgac cgtagacagc agcttcagcg gctacgactc
      841 gagcccggtg aacgacggcg tgaccgtggg cgagggcctg cattggacga aagaagcgtg
      901 ggcctccgcc gaagaggcgc gaccgcactt catcgagctg cggttcaagg aaccgaggag
      961 catcgcgcgt gcgctcgttt actggtcgct tgatgggggc gtggcgcgga cctcgcgcga
     1021 gatcgcggtg gagatcccgg agggcgatgg ttggaagcgc gtggcgcttg tgaagccagg
     1081 cgcgccggag ccagtgacgg agatccggtt cgcgcagccg gtccagtccg aacgcttccg
     1141 gttcgtccag ccgatgggtg ctggccccca gggccggccc aacctgatgt gggtgcgcga
     1201 ggtggagctc ttccccgagt ccaccgcgcg ctagcccgta ttattctctg gtggatgctt
     1261 cgggctagag ttcggatgga gcgggctgcc ggtggcgctc aggccctggt tgcccgctcc
     1321 tcggctgaag tcgcatcctc atattcgaac cgggagttac tgtgtccagc cggattcctt
     1381 ctctcgatct cagggaagtg agtggggagt ctttgatgtc ctatcgcatc agttggtttc
     1441 tagccgcgtg tttggccatc ctctgcggag cgcccctcgt agcggcgccg gccgaggtgc
     1501 ccctgaaaaa tccctccttc gaggagggtg tggatcaaag aggcactccc ctgggttggc
     1561 agcgctatgg gggcggtggc cagggccagc gcctctcggt ggtggacgtg cccggcgggg
     1621 ggaaggcgct gctgatcgag gatggcgacc cgaacgcgga gatcgggatc acccagacgg
     1681 cgcctgcgcg cccggaggag gtctaccggg cgtcggtgcg cgttgggcgc gtcgccggag
     1741 cggatgccgc cggtgcctac ctgcagctgc ggtttctccc ctctcagaag ttcgtgcagg
     1801 tggccttgat gccaggcgag ggcatggcgt tccgcgcggt gagcgccacg atgcaggctc
     1861 ccgctggcac caccgggctg atgctctatc tctacacgca caaggccccc actccgaagg
     1921 tg
//
LOCUS       DAOSCV010000901         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39953_length_1914_cov_3.698763, whole genome shotgun sequence.
ACCESSION   DAOSCV010000901 DAOSCV010000000
VERSION     DAOSCV010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39953_length_1914_cov_3.698763"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="PLY56_17510"
     CDS             complement(<1..1646)
                     /locus_tag="PLY56_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOJ23327.1"
                     /translation="MNSITATELSRILEDEILPRVEKPSRYLGCEVNATRKEAAPGDV
                     RIALAFPDLYDLGLPNLGLLILYDILNRREGVHAERVYAPGLDLEAILRERRLPLFSL
                     ESRTPLAQFDCIGFTLQYELCYTNLLNMLDLAGIPLQSSQREDRHPIILAGGPCALNP
                     EPLADFIDAFAIGDGEEVIVEIAEALRATKGVPRAERLRRLAEIEGVYVPALYETRTL
                     PDGTVLPVGPKVRKRVVALEGAPFPTEYVVPFTQQVHDRVSLEVLRGCTQGCRFCQAG
                     MMNRPVRERSLKTLRELQEAALRATGYEEIALASLSTCDYSRVRSLVAQTVEAATPWG
                     TAVSLPSLRLDSFSVDLVEMVQSMRKSGLTFAPEAATPRLRAVINKGITDEDLLETTA
                     EVFRRGWEVVKLYFMIGLPTETEEDVAAIAELSRRVLAGGRSFRRNARINLGVSTFVP
                     RPHTPFQWERQISLEETQAKQRLLAERLRGGVKFGRHDAFASYLEGVFARGDRRVGGA
                     LLEAWRRGARMDAWSEHFDFARWQEAFATVGLNPDDYLRER"
BASE COUNT          326 a          638 c          651 g          299 t
ORIGIN      
        1 cgctcgcgca ggtagtcatc gggattgagg ccgaccgtgg cgaacgcctc ctgccagcgc
       61 gcgaagtcga agtgctcact ccaggcatcc atgcgggcgc cacggcgcca cgcctccaac
      121 agggcgcccc cgacacggcg gtcgcctcgc gcgaagacgc cttccagata ggacgcgaag
      181 gcatcatgcc gaccgaactt cacgccgccg cgcaggcgct cggccaggag acgctgcttc
      241 gcctgtgttt cctcgaggga gatctgccgc tcccactgga acggcgtgtg cggccgcggc
      301 acgaaagtgg agacgcccag gttgatgcgc gcgttgcgac ggaagctgcg cccgccggca
      361 agcacgcgcc gcgatagctc cgcgatggcg gccacgtcct cctccgtctc cgtggggagg
      421 ccgatcatga agtagagctt caccacctcc cagccgcgac ggaacacctc ggcggtcgtc
      481 tctaggagat cctcatcggt gatccccttg ttgatcacgg cgcgcaggcg cggtgtggcc
      541 gcttcaggcg cgaaggtgag ccccgatttg cgcatgctct gcaccatctc caccaggtcc
      601 acggagaagg agtcgaggcg gagcgatggg agcgagacgg cggtacccca gggcgtggct
      661 gcctctaccg tctgggccac cagcgagcgc acgcgcgagt agtcgcacgt gctcaggctg
      721 gcgagggcga tctcctcgta gccggtggcg cgcagcgcgg cctcttgcag ctcgcgcagc
      781 gtcttcaggg agcgctcgcg cacgggtcgg ttcatcatcc ccgcctggca gaagcggcac
      841 ccctgcgtgc agccccggag gacctccagc gagacgcgat catgcacctg ctgggtgaag
      901 ggcaccacgt actccgtggg aaaaggggcg ccctccaggg cgacgacgcg ctttcgcacc
      961 ttcgggccga cggggagaac cgtgccgtct ggcaacgtcc gcgtctcgta gagcgcgggg
     1021 acgtagaccc cctcgatttc ggccagccgg cgcaggcgct cggcgcgagg gacgcctttc
     1081 gtggcgcgca gggcctccgc gatctcgacg atcacctcct cgccatcgcc aatcgcgaag
     1141 gcatcgatga aatcggcgag tggctcgggg ttgagcgcgc acgggccgcc ggccaggatg
     1201 atcgggtggc gatcctcccg ctggctcgac tggagcggaa tcccggccag atcgagcatg
     1261 ttgagcaggt tcgtatagca gagctcatac tgcagtgtaa agccgatgca gtcaaactgg
     1321 gccaacggcg tccggctctc tagcgagaag agtggcaggc gccgctcgcg caggatcgcc
     1381 tccaggtcaa gcccgggggc atagacgcgc tctgcatgca cgccctcgcg tcgattaagg
     1441 atatcgtaca ggatcaggag ccccaggttc ggcagcccga ggtcgtacag gtccggaaag
     1501 gcgagtgcga tccgcacgtc acccggcgcg gcttctttac gggtggcgtt cacctcgcac
     1561 cccagatagc ggctcggctt ctccacccgg ggcaggattt catcttccag tatacgcgac
     1621 aactcggttg cagtgatcga attcatgaac tctcccacgc tcattctagc agccagactc
     1681 gaggaaagtc aacctgcgac cctgcgcacc tcgcgccggc cgtgatacca cggaggaagg
     1741 ggacgggcac ggatgggacg ggatggtgcg cctggggcct ggagaaggtg tgggcgcccc
     1801 gcgcgaccca ggctgccgcc tatcgccctg gcttgcagga agagggacgc cggtgtcgta
     1861 ttaggcctat gggccccctc gagcgcgcac ggcgtggcgt gactcttttc gggg
//
LOCUS       DAOSCV010000902         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39957_length_1914_cov_3.263045, whole genome shotgun sequence.
ACCESSION   DAOSCV010000902 DAOSCV010000000
VERSION     DAOSCV010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39957_length_1914_cov_3.263045"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..389)
                     /locus_tag="PLY56_17515"
     tRNA            complement(313..389)
                     /locus_tag="PLY56_17515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(353..355),aa:Arg,seq:tcg)
     gene            570..1814
                     /locus_tag="PLY56_17520"
     CDS             570..1814
                     /locus_tag="PLY56_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23328.1"
                     /translation="MPVNSAPRDRAIIRDLAKQVAEIAALPVQQETIALWKALNARKP
                     IRPMVRIDQVCWHEMNVDDELTPRCQDSFCRSLESQLRRTLYAWRHMRGDMVVAPYVE
                     LPKAIHNTGFGLDVQERRLAADPRNTVVSHAYFDQLQTPDDLEKIRMPEVWHDEAATA
                     RTEERARELLDGILDVRMQGACPAYAPLDLITCWRGAEPLLWDLVDRPEFVHAILQRV
                     TEGQLHFLDQLEAQGLLGRDQQTIHCTGAHTDELPAPGFNPNHPRARDLWTFGMSQIF
                     STVSPAMHEEFEWPYLTPWFERFGLGYYGCCEPLDQKLPLIRKYPRVRKVSMSPWADI
                     ERGASEIGGDFVLSRKPNPAFLAAETWDPAAVERDLRETRDACARHNCPLEFILKDIS
                     TVRYQPQRLWEWAEIAMRVAGN"
BASE COUNT          335 a          689 c          592 g          298 t
ORIGIN      
        1 ctcctctccc ggccggcgtg acctggtcgc gttctgtttt tttgccctac gcctgcctgc
       61 cttcgctttg cccctgctct tctcccgcgc gcgccattct cctccttggc gcaggcgctg
      121 gaccggcacc agcaaccgga acctatgttg agaggatctg gcacacgacc tgaacctgac
      181 gtgggaatgg ggcccggcca ccggacgcgc ggaggcggtc gggagcccgc gctcgaaggg
      241 gtccaaacag agaacgggcc ctggcacccc cctctgctgg aggaggccag ggcccggatc
      301 gatgcgatac catggcgcgc ctggagggat tcgaaccccc gacacctggt tccgaagacc
      361 agtgctctga tccactgagc tacaggcgcg cgaaggaggc ttgcccatct aaggcagcca
      421 cattgtacca ttcgccggcg aatctgtcaa tcgcgatgaa gccggtgctc tcccctgaga
      481 agggggcatc tcccatccgg agaatgaatc ggggagatcc gcgcggggcg gcgcccgcca
      541 gcgaacgcca cattgcgcgg gagagggaga tgcctgtgaa cagcgcaccg cgagatcgcg
      601 cgattattcg cgacctggca aagcaggtgg ccgagatcgc tgccctgccc gtgcaacagg
      661 agacgatcgc cctatggaag gcgctcaacg cccggaagcc catccggccg atggtgcgga
      721 tcgaccaggt ctgctggcat gagatgaacg tggacgacga gctgacgccc cgctgccagg
      781 acagcttctg ccgcagcctg gagagccagc tccgccgcac cctctacgcc tggcggcaca
      841 tgcgcgggga tatggtcgtc gctccctatg tcgagctccc caaggcgatc cacaacaccg
      901 gcttcgggct ggacgtgcag gagcgccgcc tcgcagccga ccctcgcaac accgtcgtga
      961 gccacgccta ctttgatcag ctgcaaaccc cggacgatct agagaaaatc cgaatgccgg
     1021 aggtgtggca cgacgaggct gccaccgccc ggacagaaga gcgcgcccgc gaactcctcg
     1081 atggaatcct ggacgtgcgc atgcagggcg cctgccccgc ctacgcgccg ctcgacctga
     1141 tcacctgctg gcgcggcgcc gagccgctcc tctgggatct ggtggaccgg cccgagttcg
     1201 tccacgccat cctgcagcgc gtcaccgagg ggcaactcca cttcctcgac caactcgagg
     1261 cgcaggggct gctgggccgg gatcaacaga cgatccactg caccggcgcg cacaccgatg
     1321 agctgcctgc cccaggcttc aaccctaacc acccgcgcgc cagggatctc tggaccttcg
     1381 gcatgtcgca gatcttctcc acggtctcgc ccgcgatgca cgaggagttc gagtggccct
     1441 acctcacccc gtggttcgaa cgtttcggcc tcggctacta cggctgctgc gagccgctcg
     1501 atcagaaact ccccctgatc cgaaaatacc cccgcgtgcg caaggtctcg atgagcccct
     1561 gggcggatat cgagcgtggc gccagcgaga tcgggggcga tttcgtcctc tcgcgcaagc
     1621 ccaaccccgc cttcctcgcc gcggagacct gggaccccgc cgcggtcgag cgcgacctcc
     1681 gcgagacgcg cgacgcctgt gcccggcaca actgcccgct ggagttcatc ctgaaggata
     1741 tcagcaccgt gcgctaccag ccccaacggc tctgggaatg ggccgagatc gccatgcgcg
     1801 tcgccggcaa ttgagcctcg caggggccca tcccatccga cggaccaggc atctacgccg
     1861 cggcctccac gattctcccg tcgcggagct cgatcacgcg gtcggcctgg cgcg
//
LOCUS       DAOSCV010000903         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_39958_length_1914_cov_3.152232, whole genome shotgun sequence.
ACCESSION   DAOSCV010000903 DAOSCV010000000
VERSION     DAOSCV010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39958_length_1914_cov_3.152232"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..739)
                     /locus_tag="PLY56_17525"
     CDS             complement(143..739)
                     /locus_tag="PLY56_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23329.1"
                     /translation="MKLSNASTRLIDGMPRLIQAGMGIHISSARLANITSRLGALGVV
                     SGAGLRHVVVEQVRAGATEAIAAARTFPFARYVEELLAFAPGGSKHRSAVPVDHPEPR
                     LGALAKRLTTICAYVEVAMAKKGHRGKVGINVMWKCALSVLPTIYGSMLAGVDALVCG
                     AGVPMELPGIVASIRAGKDLSYTPLTGTDTHASEHRRG"
     gene            complement(1015..>1914)
                     /locus_tag="PLY56_17530"
     CDS             complement(1015..>1914)
                     /locus_tag="PLY56_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23330.1"
                     /translation="ALHRPGGVLVYGIPELRLPKAIVAEEIAALQALGVKIETNVVIG
                     RTITIDELFAEGYDAVFVGSGAGLPMFLGIPGENLCGVYSANEFLTRMNLMRAFDFPN
                     YDTPIQIGKHVAVIGGGNVAMDAARVALRMGPEKVTILYRRSRAEMPARLEEIERAEE
                     EGVHFELLVAPTRMLGDDRRWVTGVECQRMELGEPDASGRRRPVPVPGSEFVVPADTV
                     VVAIGNHPHPLVPQTTPGMAVTKKGTIVADPETGATPRPGLFAGGDIVTGAATVILAM
                     GAGRRAARAISEYLNTLPPKSDT"
BASE COUNT          341 a          660 c          603 g          310 t
ORIGIN      
        1 atggttttcg aggacgaagg cgaaaggccg cgcgccatca tagcgcttcg cccacgtatc
       61 caggatccgc ttgcagaagg cgaaattcga aacgatgggc agcagcttcg ggggctccat
      121 acgcgccagc accggcgccg ggtcatcctc ggcgatgctc agatgcgtgt gtgtctgtgc
      181 cggtgagcgg cgtgtacgag aggtccttcc cggcgcggat gctcgccacg attccaggaa
      241 gctccatcgg cacgccggcg ccgcacacca gggcatcgac gccggccagc atcgagccgt
      301 aaatcgtcgg gagcacgctc agggcgcact tccacatcac attgatgccc accttgcccc
      361 gatggccctt cttggccatc gccacctcga cataggcgca gatcgtggtc aaccgctttg
      421 ccagggcgcc caagcgtggc tcggggtgat ccaccggcac ggccgagcgg tgtttcgatc
      481 cgcctggcgc aaaagcaagg agctcttcga catagcgcgc gaaggggaag gtacgcgccg
      541 cagcaatcgc ttcggtggca ccggcacgca cctgctcgac cactacgtgg cgcaggcccg
      601 cgcccgatac cacaccgagg gcacccaagc gcgaggtgat attggccagt cgggcgctgg
      661 agatatggat tcccattccc gcctggatca ggcgcggcat cccatcgatc agccgcgtgg
      721 aggcattgct caacttcatc tcccgtcttc ctggcccagc tgggaacttc gcaaagcaac
      781 gtgttccaac accaatgcgt cggatacagc gtgagcgctt gccaggccag tgggggttgc
      841 ccgtagactc gcgcacagga gaggcacgcc aacgggtgaa ggcgcattcc cggctgtctc
      901 gccctcttcg gtagttcgac gcatcctctg gaatgccctg cttatcaggc gtatgaggaa
      961 aaggggcgag ctacctacag gcagcccgcc cacatcacag gaaaaccgtg ccggctaggt
     1021 atcactcttc gggggcaacg tgttgagata ctcgctgatg gcgcgcgcgg cgcggcggcc
     1081 ggcgcccatc gcaaggatga ccgtcgcggc cccggtcaca atatcaccgc cggcgaagag
     1141 gcccggccgc ggggtggcgc ccgtctccgg atcggccacg atggtgccct tcttggtcac
     1201 ggccatgcca ggcgtggtct gaggcaccag cgggtgcggg tggttgccga tggcgaccac
     1261 cacggtatcc gcgggcacca cgaactcgga ccccggcacc ggcaccggtc gccgccgacc
     1321 tgaggcatcc ggctcgccca gctccatgcg ctggcactcc actccggtca cccagcggcg
     1381 gtcatcaccc agcatgcgcg taggcgccac cagcagctcg aaatggactc cctcctcctc
     1441 cgcgcgctcg atctcctcca ggcgcgcggg catctcggca cgcgagcgcc ggtataggat
     1501 cgtcactttc tcagggccca tgcgcagggc cacgcgcgcc gcgtccatcg ccacgttgcc
     1561 gcctccgatc accgccacgt gcttgccgat ctggatcggg gtatcgtagt tggggaagtc
     1621 gaaggcgcgc atcaagttca tgcgcgtgag gaactcattg gccgagtaga cgccacacag
     1681 gttctcgccc ggaatgccca ggaacatcgg gaggccggcg cccgagccga cgaagaccgc
     1741 atcatacccc tcggcgaaca gctcatcgat cgtgatcgtt ctcccgatca cgacgttcgt
     1801 ctcgatcttc acgcccaggg cctggagcgc ggcgatctcc tcggcgacga tcgccttcgg
     1861 caggcggagc tctgggattc catagaccag cacgccgccc gggcgatgca gcgc
//
LOCUS       DAOSCV010000904         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40022_length_1912_cov_2.795907, whole genome shotgun sequence.
ACCESSION   DAOSCV010000904 DAOSCV010000000
VERSION     DAOSCV010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40022_length_1912_cov_2.795907"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="PLY56_17535"
     CDS             <1..1693
                     /locus_tag="PLY56_17535"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOJ23331.1"
                     /translation="LGLSGTENPNPDMSGYFLVFAVASLFAVSYHNYLRYVPVQRLGD
                     TPEVLGPVLRWQVVATAALFAGVSMLTAVVAVPLNQAGSVGVYRFRIRATPLLQSLAA
                     LRYHVMGREIRIGLGPSALSEREVFHIESSEPAYWRIRTYDRYTGNGWVDSIEGLPQR
                     VLATARGTAWEMVLPGSVDQGRGARAHREWLAQRVRVVGHWDSSALLAAAEPAVVRFA
                     TYPGPLLITPSGSVHLVNIPYLTAGLSYEVVSDVLRASPVALRSAPPASEIHDPALAA
                     MLAPYLRVPNSAWRLQAEAERLTAGLKNDYDRAAAIEAWLSGNYVYNLQVSPVPEGQD
                     AVMHFLYTAKEGYCDLFASAMVLLCRAAGIPARLATGFSGGTRDPETGSIVVRERDAH
                     AWPEVYFHGYGWVTFEPTPASGEDLISQYQKGGWSLLTSRIRRVLTFPVLILSLVGLL
                     SLHLVRLVAYDPWRRRRDLLARSGEDARGRVLRAYYTALHHLRRHYPRQAFETPAEYA
                     RTVTARAGSASWLPALEILTDLFLAARFGHDPVTEKDARLAEASLGRIVAGLKRH"
BASE COUNT          281 a          639 c          660 g          332 t
ORIGIN      
        1 cctcgggctt tctggcaccg agaaccccaa ccccgacatg tcgggctact tcctggtctt
       61 cgcggtcgca tcgcttttcg ccgtctcgta tcacaactac ttgcggtatg tgccggtaca
      121 gcgcctgggc gatacccccg aggtgttggg gcccgtcctg cgctggcagg tggttgccac
      181 ggcggcgctc ttcgccgggg tctcgatgct gacagctgtg gtagccgtgc cattgaacca
      241 ggcggggagc gtgggggtat accgcttccg gatccgggcc accccgctcc tgcagagcct
      301 ggccgcattg cgttaccacg tgatggggcg ggagatccgg atcgggctgg gccccagcgc
      361 gctttccgag cgcgaggtgt ttcatatcga gagctccgaa ccggcctact ggcgaatccg
      421 cacctatgac cgctacaccg gcaatgggtg ggttgacagc atcgagggcc tgccgcagcg
      481 cgttctggcg acggcccgtg gcactgcgtg ggagatggtg ctgcctggaa gtgtggatca
      541 ggggaggggg gcccgcgccc accgtgagtg gctggcgcag agagtgcgcg tggttgggca
      601 ttgggattcc tccgcgcttc tggctgcggc ggagccggcc gtcgtccggt ttgccaccta
      661 tcccgggcct ctcctgatca cgccctcggg cagcgtccac ctggtgaaca tcccctacct
      721 cacggcaggc ctcagctatg aggtggtatc ggacgtgcta cgggcgtcac cggtagcctt
      781 gcggagcgcg cctcccgcga gcgagatcca cgatcccgct ctggccgcga tgcttgcgcc
      841 ttacctgcgc gtgcccaaca gcgcgtggcg gctgcaggcc gaggcggaac gcctcaccgc
      901 cggcctgaag aacgactacg accgcgccgc cgccattgag gcgtggctga gcggtaacta
      961 cgtctacaac ctgcaggtga gccccgtgcc ggagggtcag gatgcggtga tgcatttcct
     1021 ctacacggcg aaggagggct actgcgacct cttcgcgagc gccatggtgc tcctctgccg
     1081 cgcggcgggc attcccgcac gcctggccac cggcttctct ggcggcacgc gggatccgga
     1141 aacggggagc atcgtcgtgc gcgagcgcga cgcgcacgcc tggccagagg tctacttcca
     1201 cgggtatggc tgggtgactt tcgagcctac cccggcttcc ggggaggacc tgatctcgca
     1261 ataccagaag ggcggctggt cgctcctcac gagccggatc cggcgcgtgc tcaccttccc
     1321 ggttctgatc ctctcccttg tgggcctgct cagcctccat ctggtccgcc tggtggccta
     1381 cgatccctgg cgcaggcggc gggacctgct cgcccgctct ggagaagacg cacggggccg
     1441 ggtgctgcgc gcctactaca cggcgctcca tcacctgcgc cggcactacc cgcgacaggc
     1501 tttcgagacg cccgcagagt atgcccggac agtcacggcg cgggccggat cggcatcctg
     1561 gctgcccgcc ttggagatac tgacggatct ctttctggcc gcgcgcttcg ggcacgaccc
     1621 ggtgaccgag aaagatgccc ggctcgcaga agccagtctg gggcggattg tggcgggtct
     1681 caagcggcac tgagcgggaa cgggccgaga ggccgcacaa agcaggaagc ccctcctctc
     1741 gggaggggct tcggtgacaa gggggagggg ccagcgttgc cgccagcggg ccggaacgtc
     1801 acttcgtcag atcgaggctg aggtcagtgc gcatgacgaa gacgtccccc tggattgagg
     1861 cgacccccga cagagtgccc agcgccttca gcgcctgctt cacggcggcg gg
//
LOCUS       DAOSCV010000905         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40131_length_1908_cov_3.842957, whole genome shotgun sequence.
ACCESSION   DAOSCV010000905 DAOSCV010000000
VERSION     DAOSCV010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40131_length_1908_cov_3.842957"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..489)
                     /gene="nuoK"
                     /locus_tag="PLY56_17540"
     CDS             complement(184..489)
                     /gene="nuoK"
                     /locus_tag="PLY56_17540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006517218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOJ23332.1"
                     /translation="MPLSWYLILAALLFCIGVYGVLARRNAIAILMSIELMLNAVNIN
                     LVAFARYTTPVMLTGQVFAIFVMMIAAAEAAVGLALVLSVYRNMRHIRVDDVRLLKW"
     gene            complement(494..988)
                     /locus_tag="PLY56_17545"
     CDS             complement(494..988)
                     /locus_tag="PLY56_17545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOJ23333.1"
                     /translation="MATQQVVFLILAGGILLSALMVVSVRNIVHAALYLALCFAGVSG
                     VYVLLRAEFLAVAQVLIYIGAITVLFLFGIMLTRHITGQNIPQLVAHWKWALAGACAF
                     FFLLLHLILRSEWRVVEEVPRDSTTALGLALVERYVLPFELASVILLAAVVGAVVLAK
                     QEGR"
     gene            complement(988..1554)
                     /locus_tag="PLY56_17550"
     CDS             complement(988..1554)
                     /locus_tag="PLY56_17550"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOJ23334.1"
                     /translation="MGLLARLRDDIFNTFVSIMKGHQVTWRNMWRERQTLQYPDERPQ
                     LPERYRGIPGVHPELCIACGACVKACPVGVISVEGRKIPGTKHRELVRYTIEAGRCMF
                     CGLCQEACPTKPVKALRMSNMFELAAGNKRSLILELPQLEAIWHTKPVEIPEEEYLAA
                     TPRKLAERDLAAAAAGEKPAKPAKEATS"
     gene            complement(1533..>1908)
                     /locus_tag="PLY56_17555"
     CDS             complement(1533..>1908)
                     /locus_tag="PLY56_17555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOJ23335.1"
                     /translation="YAGLTSATDWTFKWGTIPAAILAGIVLVAGLLGFISVVVTLMKA
                     SMLVFVLMWLRGTLPRVRVDQLMGFAWKVLVPLGLLNFLALAFQAATGFPSGWILNGL
                     FLLGLVLLVVFGWRKRWAYSHV"
BASE COUNT          410 a          662 c          559 g          277 t
ORIGIN      
        1 gagaagcggc gcaaagatga tgacgggcgc gagcgccagt gcactctcag cactcatcgg
       61 cagaccccct caccagcggt cacgaaatcg agcgcacagg acgtgggaag cgcgcgcgtc
      121 ccacccatct ccttcatcgc cccgatacct ctctttcgct ccatcactga ccccgtactc
      181 cggctaccac ttgagcaacc gcacgtcatc cacccggata tggcgcatat tgcgatagac
      241 cgagaggacg agcgccaggc cgacggccgc ctcagcagcg gcgatcatca tgacaaagat
      301 ggcgaaaacc tggccggtca gcatcaccgg ggtggtatag cgcgcaaagg ctaccaggtt
      361 gatattcacg gcgttgagca tcaactcgat gctcatcaga atggcgatgg cgttgcgccg
      421 ggccagcacc ccatacactc cgatgcagaa gaggagcgcc gccagaatca gataccaact
      481 caagggcacc acgttatcgc ccctcctgtt tcgccagcac gaccgcgccc accaccgccg
      541 ccagcaggat caccgaagcc agctcgaacg gcagcacgta gcgctccacc agcgccaggc
      601 ccaaagcagt ggttgagtcg cgcgggacct cttccaccac gcgccactcg ctgcggagga
      661 tcaggtggag caggagaaag aagaaagcac aggcgccggc cagcgcccac ttccaatgcg
      721 ccaccaactg cgggatgttc tggccagtga tgtgccgcgt gagcatgatg ccaaagagga
      781 acaacaccgt gatggcgccg atgtagatca gcacctgagc caccgccagg aactccgcac
      841 gcaacagcac gtagaccccg gaaaccccgg cgaagcagag cgccaggtaa agcgccgcgt
      901 ggacgatgtt gcgcaccgag accaccatca acgcggagag gagaatgccc cccgcgagga
      961 tcagaaacac cacttgctgc gtggccatca ggaagtcgcc tccttcgcgg gcttagccgg
     1021 cttctcgccg gcggcggctg ccgccaggtc acgctcggcc agcttgcgcg gcgtggccgc
     1081 cagatactcc tcttcgggga tctccaccgg cttcgtgtgc cagatcgcct ccagctgcgg
     1141 caattcgagg atcagcgacc gcttgttgcc ggccgcgagc tcgaacatgt tcgacatgcg
     1201 aagcgccttc accggcttgg tggggcaggc ctcctgacag agcccgcaga acatgcaccg
     1261 cccggcctca atcgtgtagc gcaccagctc gcggtgtttc gtgccgggga tcttgcggcc
     1321 ttccacggag atcaccccca ccgggcaggc cttcacacac gccccgcacg cgatgcacag
     1381 ctcgggatgc acccccggga tgccccggta gcgctccggc agctgtgggc gctcgtccgg
     1441 atattgaagc gtctgccgtt cgcgccacat gttgcgccac gtcacctggt ggcccttcat
     1501 gatgctcacg aacgtattga agatgtcgtc cctcagacgt gcgagtaggc ccaacgtttt
     1561 ctccaaccaa acaccaccag caacaccaga ccgaggagga acagtccgtt caggatccac
     1621 ccggacggga agcccgtcgc cgcctgaaat gcgagcgcca ggaagttgag cagcccgagc
     1681 ggcaccagta ccttccaggc gaagcccatc agctgatcca cgcgcacgcg cggcagcgtg
     1741 cctcgcagcc acatcagcac gaagacgagc atcgaggcct tcatcagggt gaccacgacg
     1801 gagatgaacc cgaggaggcc ggccaccagg acgatccctg ccaggatcgc cgccgggatg
     1861 gtgccccatt tgaaggtcca atccgtagcc gaggtcagcc cggcatag
//
LOCUS       DAOSCV010000906         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40162_length_1907_cov_3.677106, whole genome shotgun sequence.
ACCESSION   DAOSCV010000906 DAOSCV010000000
VERSION     DAOSCV010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40162_length_1907_cov_3.677106"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1038)
                     /locus_tag="PLY56_17560"
     CDS             complement(22..1038)
                     /locus_tag="PLY56_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ23336.1"
                     /translation="MKAKALVCSESQEFTLADVELPEMGPTDLLIRTHFSGVSIGTEF
                     ALIRNKISWGPYPLCTGYQGVGVVERVGRDVRGFAVGDKVYYRNSRGPFRMGGQAASA
                     VSGTHCSMAVTDATHATHGPDLLPEGVDEEVASTFVMPAVGYHGVDMAGVRMGDVVAV
                     QGAGLIGLGVVAAAKLRGAVVIAIDLDERKLAVACRLGADHVIQAGREEVEPALRAIA
                     PQGADVVFEATGVPALLDSAFALCRPHGKFVFQGNYGAAPISFRFLVPHGRQITAYFP
                     CDDGYAPCRRAVMHLLASGALKWGETITHRVAARDAPALYSHINRSSHDTDVLGAVIR
                     WDDL"
     gene            complement(1455..1682)
                     /locus_tag="PLY56_17565"
     CDS             complement(1455..1682)
                     /locus_tag="PLY56_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23337.1"
                     /translation="MARKPPAIGRFVVEIQVPEDELRPTVGGMLVALEEGLQTRGYRI
                     LVRSVTPEPGVPTWSILSEERRPDTPTAARV"
BASE COUNT          317 a          650 c          637 g          303 t
ORIGIN      
        1 cggactcgcc atcgcttccg tctagagatc atcccagcgg atgacggcgc cgagcacatc
       61 agtatcgtga ctgcttcgat tgatatgcga gtagagcgcg ggcgcgtccc tggcagcgac
      121 gcggtgggtg atcgtctcgc cccacttgag agctccgctt gccagaaggt gcatgacggc
      181 gcgccggcag ggagcgtagc catcatcgca ggggaagtag gcggtgattt gcctcccgtg
      241 cggcaccagg aatcggaacg agatgggagc ggcgccgtag ttcccctgga agacgaactt
      301 gccgtggggc cggcagagcg caaacgccga gtcgagcagg gcgggcacgc cggtcgcctc
      361 aaagacaaca tccgcgcctt gaggggcaat cgcgcgcagc gctggctcca cctcttcgcg
      421 cccggcctgg atcacgtgat ccgcgcccag ccggcaggcg actgccagct tgcgctcgtc
      481 caggtcgatg gcaatcacca cggcgccccg gagcttggcg gcagcaacca cgcccaggcc
      541 gatcaacccg gcgccttgca cggcgaccac gtctcccatg cgcacgccgg ccatatccac
      601 cccgtgatag cccaccgccg gcatcacgaa cgtggacgcg acctcttcat ccactccctc
      661 gggaagcagg tcggggccgt gggtggcgtg ggtggcatcg gtgaccgcca tcgagcagtg
      721 cgtgccgctc accgccgagg cggcctgccc ccccatccgg aacggaccgc ggctgtttcg
      781 gtagtagacc ttatcgccaa ccgcgaagcc acggacgtcg cggcccacgc gttccaccac
      841 gcccacgccc tggtaaccgg tgcagagggg gtaggggccc cacgagattt tgttgcggat
      901 cagggcgaac tccgtgccga tgctcacccc ggagaagtgt gttcggatga ggagatccgt
      961 aggccccatc tcgggcagct ccacgtcggc cagggtgaac tcctggctct ccgaacagac
     1021 gagcgcctta gctttcacgc gaaccctccc gaataggatc tgcgcgccgc aggccatgcc
     1081 ggccacggtc atcttccggc gcggctttgg gcaagatggg ggcgcgctgt gccaggaagc
     1141 agaacgcacg ttggaatctc gccgcggctc ggtggagctt gtggtcctcc tggcgcgtgc
     1201 cgcctggctc tctccagatt tcgcttctgg gggacaaatg cctgccggat ccgccactgc
     1261 cgcgcgagcg cgtgatcggg tgcgggagag ggcaacggga ccgcgctggt gcggtatggg
     1321 agcgatagcg cttgaggcaa ccaaaaccat cctggccgcc tgaatgcgag cggccagggc
     1381 ggaagcaggc gctggtcatt gcgccgagtt ggggcgcgcc tgagcgcgcc cttctggagc
     1441 actggatatc gctgctagac gcgcgctgcc gtgggggtat ctgggcggcg ctcttcagac
     1501 aggatgctcc aggtcggcac gccgggctca ggcgtcacag agcgcaccag gatccggtag
     1561 ccccgcgtct gaagaccctc ctccagcgcg accagcatcc cgcccaccgt ggggcggagc
     1621 tcatcctccg gcacctgtat ctctaccacg aagcgcccga tggctggggg ctttctggcc
     1681 atggcgtgac ctcctcaaaa tatgctcgcc ccccacctgc cccgcacagg tggggaggcg
     1741 agccccgtcc catagtcgct cttgaagagt cgcactggcc ggcgacgagc gacctggctc
     1801 tacacccgac ggctgcgata catcgtgtag ccgccgtggg acccagtcat gctatacatc
     1861 tggtagcgag cggctttgcg cgctcgccgg catgccgggc gccgggc
//
LOCUS       DAOSCV010000907         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40174_length_1907_cov_2.688985, whole genome shotgun sequence.
ACCESSION   DAOSCV010000907 DAOSCV010000000
VERSION     DAOSCV010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40174_length_1907_cov_2.688985"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLY56_17570"
     CDS             complement(<1..403)
                     /locus_tag="PLY56_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23338.1"
                     /translation="MAERERGEDIRITPETGQVGGVAPAAVMPAVGADRVRWGPIWAG
                     LLVAIATQMILSSLGLAIGLYSITQTAAGALGISWSAVGLWSAIWALISLFLGGWVAA
                     RLASATTNASGAWHGVIVWALSLVLGTLLTAI"
     assembly_gap    762..771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <772..1196
                     /locus_tag="PLY56_17575"
     CDS             <772..1196
                     /locus_tag="PLY56_17575"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ23339.1"
                     /translation="GRSFVSAELSGPDAGVSEALRVLSPLAESQLPLQFLRACPDCLS
                     FVVAQSDLPQVREVLDRAGVSFEASEGCAVITVVAPNMRSIPGLMARMGEVLFGRAIG
                     VYQMADSHSSVSCVIAAGQLVEAVEALRAEFGLPEEAA"
     gene            1227..>1907
                     /locus_tag="PLY56_17580"
     CDS             1227..>1907
                     /locus_tag="PLY56_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23340.1"
                     /translation="MRIKVLKFGGTSVDTAEHRQAAVKRIVQARAEGYHPVVVVSAIG
                     RAGAPYATDTLLSELRSVDPAAVPEARELDLMMACGEIISTVIMAHTLKALGYRATAL
                     TGGQAGIVTDEVFGDARIVSINPHYVISTLAEGSIPVVAGFQGVTDPGTGRHGAITTL
                     GRGGSDTTASALGAALHAEAVEIYTDVPGVMTADPRICPGTLSLPTVSYEEIAEMAHQ
                     GARVLHPRA"
BASE COUNT          317 a          580 c          661 g          339 t
ORIGIN      
        1 caatggcggt cagcagcgtg ccgagaacca gcgagagtgc ccacacgatc acgccgtgcc
       61 acgcaccgct cgcgttcgtc gttgccgaag ccagtcgtgc agccacccag ccgccaagga
      121 agagcgagat caacgcccag atagcggacc acagtccgac ggcgctccag ctgattccca
      181 gcgccccggc cgccgtttgg gtgatggagt agagcccgat cgccagtccc aacgagctca
      241 ggatcatctg agtggcgatg gcgacgagca gacctgccca gattgggccc cagcgaaccc
      301 ggtcagcacc gacggccggc atgacggcgg cgggcgccac accgcccacc tgcccggttt
      361 cgggggtgat gcggatgtcc tcgcctctct ctctctcagc cattttccgg tttccctccc
      421 tgggaagcgt cgcaactcat accggttcca gttccggaac gctgccctcc cggatggagg
      481 cgccattccg aatcgcagac ccagatttgc agtctgctat cggtatcact ccccatcggg
      541 tgttttccga tgggcgtgcc tgctaaacgt agcggcgcgg atggcgcatc gcgcatgaat
      601 attggttgac aaggtgggac cgctgtgcta tacttcaggc cggtgaaaag gatgctgttg
      661 ggaacgtgcc actgggtcgc ggtaaggggt gagggaccat ggcggaaagg atgcggccga
      721 tccgggcgcg ccgggtaggc ttcgagaggg agaggggagt tnnnnnnnnn ncaggccggt
      781 cctttgtttc tgcagagctc tccgggcccg atgccggggt ctccgaggcg ctgcgagtgc
      841 tttcaccgct ggcggagagc cagctgcccc tccaattcct gcgcgcttgt ccggactgcc
      901 tgagctttgt cgtggcgcag agcgatctgc cccaggtccg cgaggtcttg gatagggcgg
      961 gggtatcgtt cgaggcttcc gaagggtgtg ccgtcatcac cgtggtggcg ccgaatatgc
     1021 gtagtatccc ggggcttatg gcccgcatgg gagaggtgct gttcgggcgc gctattggcg
     1081 tgtaccagat ggcggattct catagctcgg tttcgtgcgt gattgcggcc gggcaactgg
     1141 tagaggcggt ggaggcattg cgcgccgagt ttggcctccc tgaagaggcg gcctagcgac
     1201 ctacccgtgg gcaggaggtt cgcaccgtga ggatcaaggt cctgaagttc gggggaacct
     1261 cggttgatac cgccgagcac cggcaggcgg ccgtgaagcg gattgtgcag gctcgcgcag
     1321 aaggctacca cccggtggta gtcgtttcgg ccattggccg ggcgggcgcg ccctacgcca
     1381 cggatacgct gctcagcgaa ctgcgcagcg tcgatccggc ggcggtgccg gaggcgcgcg
     1441 agttggacct gatgatggcg tgcggcgaga tcatctcgac ggtgatcatg gcgcacacgc
     1501 tgaaggcgct cggctatcgc gcgacggcgc tcaccggcgg gcaggcgggg atcgtcacag
     1561 atgaggtttt tggcgatgcg cgcatcgtct cgatcaaccc gcactacgtg atcagcacac
     1621 ttgccgaggg ctccatcccg gtggtggccg gcttccaggg ggtcacagac ccaggaacgg
     1681 gccggcatgg cgccattacc acgctggggc gtggtgggag cgacaccacg gcatccgccc
     1741 tgggagccgc gttgcacgcc gaggcggtgg agatctatac cgacgtgcct ggggtcatga
     1801 cggcggaccc gagaatctgt ccgggaactc tttcgctccc cacggtctcc tatgaagaga
     1861 tagcggagat ggcacaccag ggcgccaggg tcctccaccc tcgcgcg
//
LOCUS       DAOSCV010000908         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40344_length_1902_cov_2.459664, whole genome shotgun sequence.
ACCESSION   DAOSCV010000908 DAOSCV010000000
VERSION     DAOSCV010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40344_length_1902_cov_2.459664"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1425)
                     /locus_tag="PLY56_17585"
     CDS             complement(223..1425)
                     /locus_tag="PLY56_17585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ23341.1"
                     /translation="MDKALRKEILQTRRLSRNWPAEPLLGGYYTDEEIDVVVKTIRES
                     MDPTVGFGFICKEITDFEQAFAAYCGTADAVSINGAGTGIDMALMALDLQPEDEVIVP
                     TLNFRAALVAVLGQNARLVVCDVDPVTLQADPNDVERRITPRTRAIFPVHMNGLSAPI
                     DDYLEIAERHPHPKYGPPKVIGDAARALGGGYKGTKIGKRGWMTVFSFHTQKNMTTLG
                     EGGAITTDDLEAAKRLRALRQFGGSDGWGSNYKMTKVQAAVGLVQLRKLDSFIAARRK
                     VAQERTEMLKDVEGLTLPVEPPDCYHTYYLYTCLVPKEWAGEKRDRLMAMLREEYGVN
                     TVVANPPVHRVVPFVAKHTQGQETPVADELGARLFCPPTHPLMTREENEYICAAIADA
                     MERLREGV"
     gene            complement(1548..>1902)
                     /locus_tag="PLY56_17590"
     CDS             complement(1548..>1902)
                     /locus_tag="PLY56_17590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ23342.1"
                     /translation="LEFADAHPDLGLCFDDGNFLLAGEEELAALEKLAGRVVHFHCKD
                     WALSAEDGKGILAADGKRYVGAVIGEGVVPRTAAKKLQASGYTGFFSVEYEGAEDPVI
                     ATARAAANLKAILAA"
BASE COUNT          324 a          669 c          570 g          339 t
ORIGIN      
        1 ggacgaacgc gccacgggcc cgcccggcgt catcaaggag cagaaccgcc acgctcacgc
       61 agtggagcat ccggaacccg gatcccctga tggcgtaccg ctgtaaagca gacgatcgtg
      121 gaggccagta cgggtgccgc ccggccccca cgatcgccag ccaccgtgtc aacacccgtg
      181 cgcttcgttg ggccgaagcc ctcggatagg agcgggcacg cctcagacgc cctctcggag
      241 cctctccatg gcatcggcga tcgcagcgca gatgtactcg ttctcttctc gcgtcatcag
      301 cgggtgagtc ggcgggcaga agagccgggc gcccagttca tccgccaccg gcgtctcctg
      361 gccctgggtg tgcttcgcca cgaaaggcac cacgcggtgg acgggcgggt tagccaccac
      421 cgtgttcacc ccgtactcct cgcgcagcat ggccatcagg cggtcgcgct tctcgccggc
      481 ccactccttg ggcaccaagc acgtgtagag gtagtaggta tggtagcaat caggcggctc
      541 caccggaagc gtcaggcctt ccacatcctt cagcatctcg gtgcgctctt gggccacttt
      601 gcggcgcgcc gcgatgaagc tatcgagctt gcggagctgc accaggccaa ccgcagcctg
      661 caccttcgtc atcttgtagt tgctacccca gccgtcgctc ccaccgaact gccggagggc
      721 acgcagccgc tttgcggcct ccagatcgtc cgtggtgatg gcgccgccct caccgagggt
      781 ggtcatattc ttctgggtgt ggaaagagaa gacggtcatc caccccctct tgccgatctt
      841 cgtgcccttg tagccgcctc ccagcgcgcg cgccgcatcg ccaatcacct tgggaggacc
      901 atacttcggg tgtgggtggc gctccgcgat ctccaagtag tcatcaatcg gtgccgagag
      961 gccattcata tgcactggga agatggcgcg cgtgcgcggg gtgatccgcc gttccacgtc
     1021 gtttggatcc gcctggagag ttaccgggtc cacgtcgcac accaccaggc gggcgttctg
     1081 ccccaggacc gccaccaatg ccgcgcggaa gttgagcgtg gggacgatca cctcgtcctc
     1141 tggctgcaga tccagcgcca tcagcgccat atcgatgccc gtgcccgccc cattgatcga
     1201 aacggcatcc gccgtaccgc agtaggcggc gaacgcctgc tcgaagtcgg tgatctcctt
     1261 acagatgaag ccaaagccga ccgtggggtc catcgattcg cgaatcgtct taacgacgac
     1321 gtcgatctcc tcatccgtat agtagccacc caacaatggc tcggccggcc agttgcggct
     1381 caagcgccgc gtctgcagga tctccttgcg aagtgctttg tccatggtca tctccttggt
     1441 gcccgtggta gcgcaagccg cgtgggggag atgcgccacg ccaatctccc cctatcactg
     1501 cggtcagcgc gagcgccacg gtcgccggcg cattccacac cctactatca tgccgcgagt
     1561 atcgccttca gattggccgc ggcgcgcgcg gtcgcgatca ccggatcctc cgcgccctcg
     1621 tactcgaccg agaagaaacc ggtgtagcca ctcgcctgca gcttcttcgc ggcagtgcga
     1681 ggcaccacgc cctcgccgat gacggcgccc acataccgct tgccatcggc cgccagaatc
     1741 cccttcccat cctctgcgga gagggcccag tccttgcagt ggaagtggac gacgcggcct
     1801 gccagcttct cgagcgccgc cagctcttct tctccggcga gcaggaagtt gccgtcgtcg
     1861 aagcagaggc ccaggtcggg atgggcatcc gcaaactcca gc
//
LOCUS       DAOSCV010000909         1898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40452_length_1898_cov_3.398806, whole genome shotgun sequence.
ACCESSION   DAOSCV010000909 DAOSCV010000000
VERSION     DAOSCV010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40452_length_1898_cov_3.398806"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PLY56_17595"
     CDS             complement(<1..690)
                     /locus_tag="PLY56_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23343.1"
                     /translation="MIRTVALLLLLLFSGWRASAAPAEYICRITPQAPVIDGRLDDAC
                     WKNAAAPPQFYELGVGGKPVKNATRVRFLMDGEALYAGIEAVTTPGKLPFGRERGRDG
                     KTWYDDCVEIFLCPSFVDPTVTQLCLTAYGAMTDLRRGPGIAPDAAIQWNGAWQAVAT
                     AREGGWSAEVRIPWSDFGVKPPPRGWVWRVKVGLVAKGYPNAMWPRNESQGFANPECW
                     GYLIFGDPNLQE"
     gene            complement(798..>1898)
                     /locus_tag="PLY56_17600"
     CDS             complement(798..>1898)
                     /locus_tag="PLY56_17600"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23344.1"
                     /translation="RRIAPWAVVLAAGIGWELVTKTKALDGTPLPDFNNPPPNRPSHH
                     VSVNPYLYACLAEESCLEAGVTLHYHEVVTTAEARGDRWLVESVGKGVRREVLAREVI
                     DCSGDADVVGLLGFERVKGEVRQPGTLEFRLEGYDYKALDADLLQARYEEALRDGTLI
                     RGDFCYARRPFIDYLRAGGANLQHLFGADSTTAETQSEANIAGRQGLLRMLRFLKTLP
                     GCEKVRIARMYPMAAIRETYRIVGETTITREDYLSGRRFDDAVAYTFYFIDVHTEEGT
                     DHEFLEEGVVPTLPLGALVPKGSQRLLVAGRTVSSDRRANSALRVEASCMAMGQAAGA
                     AAALGVRQGVPSRDVPLDLLRDTLRAHGALVP"
BASE COUNT          306 a          685 c          611 g          296 t
ORIGIN      
        1 ctcctggaga ttgggatccc cgaagatcag gtagccccag cactccgggt tggcaaaccc
       61 ctggctctcg ttgcgcggcc acatggcgtt tggatagccc ttcgccacca ggcccacctt
      121 cacgcgccac acccatccgc gcggcggcgg cttcacaccg aagtcgctcc aggggatgcg
      181 cacctcggcg ctccagccac cctcgcgagc cgtggcaacg gcctgccacg cgccgttcca
      241 ctggatcgcc gcgtctgggg caatgccagg gccacggcgc aggtccgtca tggcgccgta
      301 ggccgtaagg cagagctgtg tcaccgtggg atcgacgaaa gaagggcaga gaaagatctc
      361 gacgcagtcg tcgtaccacg tcttcccgtc acggccacgc tcgcgcccga aggggagctt
      421 gccgggcgtc gtcaccgcct cgatgccggc gtagagcgcc tcgccatcca tgaggaaacg
      481 cacccgggtc gcgttcttga caggcttccc gcccacgccc agctcataga actgcggcgg
      541 ggccgccgcg ttcttccagc aggcatcatc caggcgcccg tcgatgaccg gcgcctgggg
      601 ggtgatccgg cagatgtact cagccggcgc ggcgctcgcc cgccagccgg aaaagagaag
      661 cagcagaaga agcgcaacgg tgcggatcat gggaactact ccttgccccc tgcttcgctg
      721 ctcctgcagg cgttccctgc cggttgacat cccgcgcccg catcccccaa gcgccctttc
      781 tctccagccc ataccctcta tggtacgagg gcgccgtgcg cgcggagagt atcgcgcagg
      841 aggtcgagcg gcacgtcgcg cgagggcacg ccctggcgca cgcccagggc ggcagcggcg
      901 ccggcggcct ggcccatcgc catgcacgag gcctccacac gcaacgctga gttggcccgc
      961 cggtcgctgg agaccgtgcg ccccgccacc aggagtcgct ggctcccctt gggcacaagc
     1021 gcgccgaggg gcagcgtggg caccacgccc tcttcgagga actcgtggtc cgtaccctcc
     1081 tccgtatgca cgtcgatgaa gtagaatgtg taggccacgg cgtcgtcgaa gcggcgcccg
     1141 ctcaggtagt cctcgcgggt gatcgtcgtc tcgcccacaa tccggtaggt ctcacggatg
     1201 gcggccatgg ggtacattcg agcgatccgc accttctcgc agccgggcag cgtcttcagg
     1261 aagcggagca tgcgcagcag gccctggcgt ccggcgatgt ttgcctcgct ctgcgtctcg
     1321 gcggtggtag agtccgcgcc gaagaggtgc tgtaggttgg cgccacccgc gcgaagatag
     1381 tcgatgaacg gccggcgcgc gtaacagaag tcgccccgga tgagcgtgcc gtcgcgaagc
     1441 gcctcctcgt aacgcgcctg aagtaggtcg gcatcgagcg ccttgtaatc gtagccctcc
     1501 agacgaaact ccagcgtgcc gggctggcgg acctccccct tcacgcgctc gaacccgagc
     1561 aggcccacga cgtcggcgtc gccgctgcag tcaatcacct cgcgtgccag cacctcccgg
     1621 cgtaccccct tgccaacgct ctccaccagc cagcggtcgc ccctcgcttc cgcggtcgtc
     1681 accacttcat ggtagtgcag cgtcactccc gcctccaggc agctctcctc ggccaggcag
     1741 gcatacagat aggggttcac cgagacatga tgggagggcc gattcggcgg cgggttgttg
     1801 aagtcgggca gcggggtacc gtccagcgcc ttcgtcttcg tgaccagctc ccagccgatg
     1861 ccggcggcca ggacgacggc ccacggggcg atccgtcg
//
LOCUS       DAOSCV010000910         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40488_length_1897_cov_3.634636, whole genome shotgun sequence.
ACCESSION   DAOSCV010000910 DAOSCV010000000
VERSION     DAOSCV010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40488_length_1897_cov_3.634636"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1230
                     /locus_tag="PLY56_17605"
     CDS             385..1230
                     /locus_tag="PLY56_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ23345.1"
                     /translation="MLPLPVGGRASVITYVENLNPVVQALVGTLFTWFMTALGAGAVF
                     IRKEMSRRMLDGMLGFASGVMIAASFWSLLAPAIELAEGQALPAWVPAVVGFLLGGAF
                     LRLADRLLPHLHSGGPSASPEGVPTSWRRSTLLILAITLHNFPEGLAVGVAFGAAAAG
                     MSGATVAGALALAVGIGIQNLPEGMAVSVPLRRDGLTRLKSFWYGQISGMVEPVAGVL
                     GATAVIVAQPILPYALAFAAGAMIFVVAEELIPEGGRNGNLHTATTGVMLGFALMMLL
                     DVALG"
     gene            1470..1793
                     /locus_tag="PLY56_17610"
     CDS             1470..1793
                     /locus_tag="PLY56_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOJ23346.1"
                     /translation="MAKRIFVGGLSYGTTSEGLQQAFESVGPVASAEICTDRETGRSR
                     GFGFVEMQNEADTATAIEQLNGTDLDGRTLTVNEARPRADRGGSRGGFGGGGRSFGGG
                     GRNRW"
BASE COUNT          285 a          563 c          708 g          341 t
ORIGIN      
        1 gtctcaaaac ggcggtggct gggaggagaa gccccgaggg ggctttgccg gaaagtagcg
       61 cggagcttcg agctccgcgc gtgagggcgg ccttctcctt atgtcggaat ctcaggccag
      121 cccgccagcc ccgcaccggc cccggtaccg cggatgaaag ggacgaaaac ggatggggga
      181 gtgcatgcga ttggcgcgcg cacgggcgcg gccccctttc ccgctccctc tatctgtgcc
      241 catctgcgga tactgatccg cccgcgaaaa gcgcgggacg gtcactcgtg tggcggactg
      301 ggagatagga ggaaggcggg ctcgcgaggc ggaatcggag ggcagaaagg gagtgtccgg
      361 cgccgccggc gcctgtcccg agggatgctt cctttgcccg tgggagggag agcatcggtg
      421 atcacctacg ttgagaacct caatcccgtg gtgcaggccc ttgtgggcac gctcttcacg
      481 tggttcatga cggcgttggg cgctggcgcc gtctttatcc ggaaggagat gagccgcagg
      541 atgctcgacg gcatgctggg gttcgcttcc ggggtgatga tcgcggcgag tttctggtcg
      601 ctgttggcgc cggccatcga gctggcggag ggccaagcgc tgccggcctg ggttcccgcc
      661 gtggtggggt ttttgctggg cggcgccttc ttgcgcctgg cggatcgcct gttgccgcac
      721 ctgcattccg gcgggccatc cgcatcgccg gaaggggttc cgacctcgtg gcggcgtagc
      781 actctcttga tcctggcgat cacgctgcat aacttcccgg aggggctggc cgttggcgtg
      841 gcgtttggcg cggcggcggc ggggatgtct ggcgccacgg tcgccggggc gctcgcgctc
      901 gcggtgggga ttggcatcca gaacctcccc gagggaatgg ccgtatcggt gccgttgcgc
      961 cgggatgggc tgacacgcct aaagagcttc tggtacgggc agatctccgg gatggtggaa
     1021 ccggtggccg gggtcctggg ggcgaccgcg gtgatcgttg cccagccgat cctcccctac
     1081 gcgctggcgt ttgcggcggg cgcgatgatc ttcgtcgtcg ccgaggagct gatcccggag
     1141 ggcgggcgca acgggaacct ccacaccgcc accaccggcg tcatgcttgg cttcgccctg
     1201 atgatgctcc tggatgtcgc gctcgggtga ggccgcgccg gctgggcgcg gggcgtggct
     1261 acccaccgtg cgccacatcg gcgcgggtgt gtccagaaaa tttcgcttgc atcttccgtg
     1321 gaaagcggtt ataatgccca cgtgcatggc cgtcttccaa cctcgggcgg ccaaagagga
     1381 gtgtggttcg tgttggacca ggatggttcc gatcccgaac tgtgctttcg aggcgcacaa
     1441 gactggttga ggtgggaaag gagccatcaa tggcaaagcg gatttttgtg ggaggtctct
     1501 cctacggaac aacctcggag gggctgcagc aggcgttcga gagcgtcggc cccgtggcat
     1561 cggcggagat ctgcaccgat cgtgagacgg gtcgcagccg cggcttcggg tttgttgaga
     1621 tgcagaacga ggcggatacg gccacggcaa tcgagcagct caacggcacg gatctcgatg
     1681 ggcgcacgct gaccgtgaat gaagcccgcc cgcgcgcgga tcgcggtggc agccgcggcg
     1741 gctttggcgg cggaggccgc agcttcggcg gcggcggtcg caaccgctgg taagcagtcc
     1801 cggtccatcc ggataaagga cgagagggca gccccccctt gggggagctg ccttttttcg
     1861 tgtacgcgcg agatagactc cgcgccgggc ccaagga
//
LOCUS       DAOSCV010000911         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40590_length_1894_cov_3.287113, whole genome shotgun sequence.
ACCESSION   DAOSCV010000911 DAOSCV010000000
VERSION     DAOSCV010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40590_length_1894_cov_3.287113"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLY56_17615"
     CDS             <1..963
                     /locus_tag="PLY56_17615"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ23347.1"
                     /translation="QREGRVDLRHVPLITIDPADVERKITPHTKAIVPVHMIGTCADM
                     DAIMAIARKHGLLVIEDAAQACGASYKGRRVGTIGDFGCFSISSYKITGGGEAGLVMT
                     HDPYLYTRAQNNHDTGACWRPDRYAKEREAGELFCGYNYKLSELEGSVNLVQLRKMPD
                     QVRRWQKTKRQITRQLRHFAGITPQQVRDPDGEVGNHLVFFAPDAQAAEKTAAALAAE
                     GVGASTRGCKSARDWHLYAFWEHILERKSATPAGCPWSCEKNAGLGQDYSPDMCPRTL
                     DILSRAVFIGINQWWTERDCDQIAGAINKVLEAYYEPLDGEIGW"
     gene            1122..>1894
                     /locus_tag="PLY56_17620"
     CDS             1122..>1894
                     /locus_tag="PLY56_17620"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ23348.1"
                     /translation="MIDALAFLGKGRRPATADLYGHDAETLLEEMDHKGVREAVVHHN
                     LSWLSHPFDGNAETMRLIAGKPRLRPCWALLSKASREYGSYEAYIEEAVAQGVRAFSF
                     FPRFGALPAAYDTSLREVVRAGTFAPLEERGLPLFIDFSGDPGAGVDCTDYEALRFLL
                     GAHPRLPVILCEYRLRAGNRVFPGVMDDHPNLHLETSGLWNYRSLEFVAHHWGAERLI
                     YGSRTPWRSTGLALGMVTMAALTARERALILAGNIRRLM"
BASE COUNT          336 a          651 c          618 g          289 t
ORIGIN      
        1 cagcgcgaag gccgcgttga cctccgacac gtgccgctga tcaccatcga cccggccgac
       61 gtagaacgca agatcacgcc gcacacgaag gcaatcgtgc ctgtgcatat gatcggcacc
      121 tgcgctgata tggacgcgat catggcgatt gcccgaaagc acggcctcct ggtgatcgaa
      181 gacgccgcgc aggcgtgtgg ggccagctac aagggaaggc gcgtcgggac gattggcgat
      241 ttcgggtgct tctccatcag cagctacaag atcaccggag gcggcgaggc cggcctggtg
      301 atgacccacg atccctacct ctacacccgc gcccagaata accacgacac cggcgcgtgc
      361 tggcgcccag accggtatgc gaaggagcga gaggcaggcg agctgttctg cggctacaac
      421 tacaagctct cggagctaga gggctccgtc aatctcgtgc agctgcggaa gatgccggac
      481 caggtgcgcc gctggcagaa aaccaaacgc cagatcacgc gccagctccg ccattttgcg
      541 gggatcaccc cgcagcaggt gcgcgatcct gacggcgagg tcggcaacca cctcgtcttc
      601 ttcgcgcccg acgcacaggc agccgagaag acagcggccg cgctcgccgc cgaaggcgtt
      661 ggcgccagca ctcgcgggtg caagtccgcg cgtgactggc acctctacgc cttctgggag
      721 cacattctcg aacggaaatc cgccacgccg gccggctgcc cctggagctg cgagaaaaac
      781 gccggcctgg gccaggacta ctcgcccgac atgtgcccgc gcacgctcga catcctcagc
      841 cgcgccgtct tcatcgggat caaccagtgg tggaccgaga gagactgcga ccagattgcc
      901 ggcgcgatca acaaggtgct ggaggcctat tacgagcctc tcgacggcga gatcggctgg
      961 taggcacgcg agactccgga cagctgaccg gccggggagc ccgcgcccgc agtcgaagcg
     1021 cgggctcccg atagtccggg ccgggcgtct gtaagcgatg aagggaggcc accctgcatc
     1081 cccagatcgt tgaaagcgaa gcgttcaccg agatcccgga agtgatcgac gcgctcgcct
     1141 tccttgggaa gggccggcgc ccagccaccg cggatctcta cggccacgac gcggagacgc
     1201 tcctcgaaga gatggaccac aagggcgttc gcgaggcggt cgtccaccac aacctgtcgt
     1261 ggctctcgca ccccttcgat ggcaacgccg agacgatgcg cctcatcgcg ggaaagcccc
     1321 gtctccgccc ctgctgggcg ctcctctcga aggcttcacg cgagtatggg tcgtatgaag
     1381 cgtacatcga ggaggcggtc gcccaaggcg tacgcgcctt ctcgttcttc ccgcggtttg
     1441 gcgccctgcc ggcggcctac gacacgtcgc tccgcgaggt ggtgcgcgcg ggcaccttcg
     1501 cgcccctgga ggagcggggc ctgccgctct tcatcgattt ttccggagac ccgggcgccg
     1561 gcgtcgactg caccgactac gaggcgctcc gcttccttct tggcgcgcac ccgcggctgc
     1621 cggtgatcct gtgcgaatac cggctccgtg cgggcaaccg cgtcttcccc ggcgtgatgg
     1681 atgaccaccc caatctccat ctggagacgt ccgggctgtg gaactaccga tcgctggagt
     1741 tcgtggcgca ccactggggc gcggagcggc tcatctatgg cagccggacg ccctggcgga
     1801 gcaccggcct ggcgcttggc atggtgacga tggccgcgct caccgcgcgc gagcgcgccc
     1861 tgatcctggc gggcaacatc cgccggctga tgga
//
LOCUS       DAOSCV010000912         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40668_length_1892_cov_2.450735, whole genome shotgun sequence.
ACCESSION   DAOSCV010000912 DAOSCV010000000
VERSION     DAOSCV010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40668_length_1892_cov_2.450735"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="PLY56_17625"
     CDS             <1..1786
                     /locus_tag="PLY56_17625"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ23349.1"
                     /translation="KNHASVIVWSLGNESGYGENHAAMSEWIRGRDATRPIHYERAIE
                     TAEAPAPASVDVVSQMYTSVENLIKQGERTDEPRPFFLCEYAHAMGNGPGNLKEYWEV
                     IRKYPRLIGGCIWEWVDHAIRTRNAEGQEYLAYGGDFGDFPNDGNFCVDGLNFPDRIP
                     HTGLIEYKHIIQPVHIEALDLEHGRIRIVNRHFHRSTDYLQGSWMLLQDDLILDQGEI
                     LDLDIPAGEAVEYQLPYTLPDPEPGATYWLNLSFTLAEEMPWASRGYEVAYDQFELPR
                     KAEAPALLAEAMPPVALRDLPGEVILHSDEFAITFDKRTGTIRDWIWQEIPLIDAGPR
                     IQLWRTPTDNDRNIKHVWYRAGYDRLVSRVQRMEVQELSRHAIRLEVDTRLGAVSLRP
                     SFDVRYCYTLYGSGDLVIETRLIPLQEDLPDLPRVGLELRTLPGFTQFAWYGRGPHES
                     YVDRKESARVGVYAGTVEEQYVPYLFPQENGNKADTRWAAITNLRGEGLLCMGMPVIN
                     VSVHHYTTEDLTASAHPHELTRIEETVVHLDYQQGGLGSNSCGPRPLPQYLLQPVEMS
                     FAVRIKPFSMDARSPMSLWKESLPAPHA"
BASE COUNT          342 a          615 c          617 g          318 t
ORIGIN      
        1 caagaaccac gcttcggtca tcgtctggtc actgggcaac gagtcgggct acggcgaaaa
       61 ccacgcggcg atgtcggagt ggattcgcgg gcgggatgcc acccggccga tccactacga
      121 gcgcgccatc gaaaccgcgg aggcgccggc gccggcgagc gtggatgtcg tcagccagat
      181 gtacaccagc gtcgagaacc tgatcaagca gggcgagcgc acggacgagc cgcgtccctt
      241 cttcctctgc gagtacgccc acgcgatggg caatggcccc ggcaacctga aggagtactg
      301 ggaggtcatc cgcaagtatc cccggctgat cggcgggtgc atctgggagt gggtggacca
      361 cgcgatccgc acgcggaatg ccgaagggca ggagtacctc gcatatggcg gcgacttcgg
      421 cgacttcccg aacgatggca acttctgcgt ggacggcctc aacttccccg accggattcc
      481 gcacaccggt ctcattgagt ataagcacat catccagccg gtgcatatcg aagcgctcga
      541 tctggagcac ggacgcatcc gcatcgtcaa ccgtcacttc catcgctcga ccgactacct
      601 gcagggctcc tggatgctgc tgcaggacga cctgatcctg gaccagggag agatcctgga
      661 tctcgatatc cccgccggcg aggcggtgga gtatcagctt ccctacaccc tgccggatcc
      721 cgagccgggc gccacttact ggctcaacct cagcttcact ctggcggagg agatgccctg
      781 ggcgtcgcgg ggatacgagg tcgcctacga ccagttcgaa ctcccgcgca aggcagaggc
      841 gccggcgctc ctggccgagg cgatgcctcc ggtggccctg cgcgacctcc cgggcgaggt
      901 gatcctccac agtgatgagt ttgcgatcac cttcgacaaa cggaccggga cgatccgcga
      961 ttggatctgg caggagatcc cgctcatcga tgccgggccg cggatccagc tctggcgcac
     1021 gccgacggat aacgaccgca acatcaagca cgtgtggtac cgcgccggct acgaccgcct
     1081 ggtgtcccgt gtgcagcgga tggaggtgca ggagctctcg cgccacgcca ttcgcctgga
     1141 agtggatacc cgcctcggcg ccgtctcttt gcggccatcc ttcgatgtgc gctactgcta
     1201 caccctctat ggctcgggcg atctcgtcat agagacccgc ctgataccgc ttcaggagga
     1261 tctgccggac ctgccgcggg tgggcctgga gctgcgcacg ctgcccgggt tcacgcagtt
     1321 cgcgtggtat gggcgtggtc cgcacgagag ctatgtcgac cgcaaggaga gcgcccgcgt
     1381 cggggtttac gcgggcacgg tcgaggagca gtacgtgccc tacctcttcc cgcaagagaa
     1441 tgggaacaag gcggatacgc gctgggcggc gatcaccaac ctgcgcggtg aaggcctcct
     1501 ctgcatgggg atgccggtga tcaacgtcag cgtgcatcac tacaccacgg aagacctcac
     1561 cgcgagcgcg catccgcacg agttgacgcg catcgaggag acggtggtgc atctcgatta
     1621 ccagcagggc ggtctgggaa gcaacagctg cggcccgcgc ccgctgccgc agtacctgct
     1681 ccagccggtc gagatgagct ttgccgtgcg catcaagcca ttctcgatgg acgcgcgctc
     1741 gccgatgtcg ctttggaagg agagtctgcc ggccccgcac gcctgatccg cggggtgccg
     1801 gcaacgcgct ggcgctctcg ctccatagtt cgggagcgcc agcagtcgtc agaagcgcct
     1861 ggggcgcttc gagttgtgaa gcaggtgatt at
//
LOCUS       DAOSCV010000913         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40753_length_1889_cov_4.242639, whole genome shotgun sequence.
ACCESSION   DAOSCV010000913 DAOSCV010000000
VERSION     DAOSCV010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40753_length_1889_cov_4.242639"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="PLY56_17630"
     CDS             <1..1062
                     /locus_tag="PLY56_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ23350.1"
                     /translation="TVVEDYGRLMDLAGLRQHVPMAFDTILKINISWHHYFPGCSTTP
                     WQFDGVIRKLRQEGYENLIPAQNRTVVVDPKVGAVRNHLRPVVEKHGMEFTYLYEPGV
                     TWVNYQPRARMRVLHDVFPDGILIPELLIGRNAIHLPTTKTHVFTGITGAMKNAFGGL
                     LRENRHWTHAVIHETLVDLLAIQKEIHPGLFAVMDGTIAGDGPGPRAMMPYVKNVILA
                     SADQVAIDAIEAKLMGFDPLSFDFIRLAHEDGLGVGDPAEIEIVGADISEVNWHFNHK
                     RETFASRGQKLIYHGPLKPLEHFLLRSPIAPWSYLASRVYHDGYWFPFVGRKRVARIM
                     ATPWGELFRAYGRPETIPG"
     gene            1106..>1889
                     /gene="hemW"
                     /locus_tag="PLY56_17635"
     CDS             1106..>1889
                     /gene="hemW"
                     /locus_tag="PLY56_17635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOJ23351.1"
                     /translation="MQAGIYIHIPFCARKCSYCDFVSWPVAAGDERIEAFTDGVCREI
                     EIVARVHAASAPLDAPTLFFGGGTPSLLSPEQLGRILAAVRAHFRLAADAEITLESNP
                     GTLDSDKARAFRDLGVNRISVGVQSFHDSELRALGRIHTAEEGAAAIETLRAAGFDNV
                     SLDLMFAIPGQSLESWRETLRRAIALRPAHISAYSLIIEPETPFERWEREGRLHRPDE
                     DAEAEMYEETIATLCAAGYEHYEVSNFALPCRRSRHNQIYWRN"
BASE COUNT          338 a          608 c          603 g          340 t
ORIGIN      
        1 accgtggtag aggactatgg gaggttgatg gacctggccg gcttgcggca acacgtgcca
       61 atggcgttcg acaccatcct gaaaatcaac atctcgtggc accactactt cccaggctgc
      121 tccacgacgc cctggcaatt tgacggcgtg atccgcaagc tgcgccagga gggctatgag
      181 aacctgatcc cggcgcaaaa ccgcaccgtg gtcgtcgatc ccaaagtggg cgcggtgcgc
      241 aaccaccttc gccccgtcgt ggagaagcac ggcatggagt tcacctacct ctacgagccg
      301 ggcgtgacct gggtgaacta tcagccgcgg gcgcgcatgc gcgtgttgca tgacgtcttt
      361 cccgacggga tcctcatccc ggagctgcta atcgggcgaa acgccatcca cctgccaacg
      421 acgaagacgc acgtcttcac cggcatcaca ggcgcgatga agaacgcctt cggcggcctc
      481 cttcgcgaga accgccactg gacgcacgcc gtgatccatg agacgctcgt cgatctcctc
      541 gccatccaga aggagattca cccgggcctc ttcgcggtga tggatggcac catcgcgggc
      601 gacggccctg gcccccgcgc gatgatgccc tacgtcaaga acgtgatcct ggcaagcgcc
      661 gaccaggtcg ccatcgatgc catcgaggcg aagctgatgg gcttcgatcc cctctccttc
      721 gacttcatcc gcctggcgca tgaggatggt ctcggcgtgg gcgacccggc cgagatcgag
      781 attgtgggtg ccgatatctc cgaggtgaac tggcacttca accacaagcg cgagacgttc
      841 gccagccggg ggcagaagct gatctaccac gggccattga agccgctgga gcatttcctc
      901 ttgcgctcgc ctatcgcacc gtggtcctat cttgcctccc gagtctacca cgacggctat
      961 tggttcccct tcgtcgggcg caagcgtgtc gcgcggatca tggccacgcc ttggggtgag
     1021 ctctttcgcg cctatgggcg ccctgagacg attccgggat aaacccagag gcgcgcgaat
     1081 cgcccgcagg tgctataatg tccctatgca ggccggcatc tacatccata taccgttttg
     1141 cgcgcggaaa tgcagctatt gtgattttgt ctcctggcct gtggccgccg gggacgagcg
     1201 gatcgaggcg tttaccgatg gcgtctgccg cgagatagag atcgtcgcgc gggtacatgc
     1261 cgcttcggcg ccgctggacg cgccgacgct cttcttcggc gggggcacgc cctccctcct
     1321 gagccccgag cagctggggc ggatcctggc ggctgtgcgc gctcacttcc gcctggcagc
     1381 agatgcggag atcacgctcg agagcaatcc cggcacgctc gattccgaca aggcgcgcgc
     1441 gttccgcgac ctcggggtga accggatcag cgtgggggtg cagagcttcc acgattccga
     1501 gctgcgggca ctcgggcgga tccacactgc ggaggagggg gccgcggcaa tcgagaccct
     1561 tcgcgctgcg ggcttcgata acgtgagcct cgatttgatg ttcgccatcc cggggcagag
     1621 cctggagagc tggcgagaga cgttgcggcg ggcgatcgct ctgcgcccag cgcatatttc
     1681 cgcctacagc ctgatcatcg agccggagac gccattcgag aggtgggagc gcgagggcag
     1741 gcttcatcgc ccggatgagg acgcggaggc ggagatgtat gaggagacga tcgcgaccct
     1801 ctgcgccgcg ggctacgagc attacgaggt ctctaacttc gcgcttccgt gccggcgcag
     1861 ccggcacaac cagatttact ggcgaaacg
//
LOCUS       DAOSCV010000914         1889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40757_length_1889_cov_3.288441, whole genome shotgun sequence.
ACCESSION   DAOSCV010000914 DAOSCV010000000
VERSION     DAOSCV010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40757_length_1889_cov_3.288441"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1363)
                     /locus_tag="PLY56_17640"
     CDS             complement(182..1363)
                     /locus_tag="PLY56_17640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23352.1"
                     /translation="MPEPLKVGVVGLGIRGFWVAHITRGCQETRLVAVADMRPEIREV
                     AREKFPDVELYASGEEMARDASIDAVFVTTGDRFHAGNAREALRSGKHVLIEKPMAQS
                     FADLQEIARLQHETGLTVGTFLELRHSRLWQRTRELLDSGTIGRVLAAALVDHVGRDR
                     NQFFARHKARSRDQVVSLVLQKGVHSLDLLNWFVGASPCRVSASGGLLFFGGKEPAEK
                     QCRHCATSGECPHFHQSTSRLPALDLTLEHGEDLCVWSQAADVEDVSLLNIEYANGVV
                     ASYREVHFAPYYGLHFTLYGEKGQLDVEANHDTGEAWIEVTERYTRRQYRERPTRDTG
                     HTNADAALITDFVQAARAGREPLSGLRAGFESAAIAIGCRQSLDTRAFVDLPMLEGNP
                     A"
     gene            complement(1508..>1889)
                     /locus_tag="PLY56_17645"
     CDS             complement(1508..>1889)
                     /locus_tag="PLY56_17645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ23353.1"
                     /translation="NKKKPGVVTLPSGLQYKVLKEGTGKSPKVTDTVTVHYRGTLIDG
                     TEFDSSYRRNEPATFEVGQIIPGWVEALQLMKTGAKWQLFIPSELAYGEQGAGSQIGP
                     NSVLLFEVELLSIGEPETPQQPAK"
BASE COUNT          314 a          611 c          622 g          342 t
ORIGIN      
        1 cccaaccgcg cctgctggct ggggctacag gcaaacaggc acgcaccggc tcccagcggg
       61 gtggcgggtt cccgcgacag cttcctattg ggatcaagtt cgcgcaatgc gcaggtattc
      121 cttgagaaca ggattcctgc cgacgctcag ctggccggcg gggcctgtct ctcctcgccc
      181 ctcaggccgg gttgccctcg agcatcggga gatcgacgaa agcacgggtg tcgagcgact
      241 ggcggcagcc gatagcgata gcggcactct cgaagcctgc gcgcaggccg gagaggggct
      301 cgcgacccgc acgtgctgcc tgtacgaagt cggtgatcag cgccgcgtca gcattcgtat
      361 ggcctgtatc ccgcgttggc cgctcgcggt actgccggcg ggtgtagcgc tcggtgacct
      421 caatccacgc ttccccggta tcgtggttgg cctcgacatc cagctgcccc ttctccccgt
      481 agagggtgaa gtgcagcccg tagtacgggg cgaagtggac ctcgcggtag ctggccacaa
      541 caccgttggc gtactcgata ttcagcagcg acacgtcctc gacatcggcc gcttgtgacc
      601 agacgcacaa atcctcgcca tgctccagtg tgaggtcgag cgcgggcagc cgactggtgc
      661 tctggtggaa gtgtgggcac tcgcctgagg tggcgcagtg gcggcactgc ttctcggcgg
      721 gctccttgcc accgaagaaa aggaggccac cgctggcact cacccggcag ggcgaggcac
      781 ccacgaacca gttgaggagg tccaggctat gcacgccctt ctgcaggacc aggctcacta
      841 cctgatcgcg agaacgcgct ttgtggcgag caaagaactg gttacggtcg cggccaacat
      901 ggtcgaccaa cgccgcggca agcacgcgcc cgatcgtgcc ggaatcgagg agctcgcgcg
      961 tgcgctgcca gagccggctg tggcggagtt cgaggaaggt gccgacggtg aggcccgtct
     1021 cgtgttgcag ccgggcgatc tcctgaaggt ccgcgaagct ctgcgccatc ggcttctcga
     1081 tgagcacgtg cttgccggat cgcagtgcct cgcgcgcgtt ccctgcatgg aaccggtcgc
     1141 cggtagtgac gaacacggca tcaatcgatg cgtcgcgcgc catctcttcg ccagaggcgt
     1201 agagctccac atcggggaac ttctcgcgcg cgacctcgcg gatttccgga cgcatatcgg
     1261 ccaccgccac caggcgcgtc tcctggcatc cccgggtgat gtgggcgacc cagaatccgc
     1321 ggattcccaa cccgacgact ccaaccttca gcggttcagg cataggggtt tcctcctgca
     1381 cctattttag cacccgcctg ctcgctcgtc aaacgcgcgg attccggcga tagcacggat
     1441 gagggatgga tgtagggaga gatgtgggga gggggcgccc cctccccttc gtggacgtgg
     1501 gagccgctta ctttgccggc tgctggggag tctcgggctc gccgatggag aggagctcga
     1561 cctcaaagag gagcaccgag ttggggccga tctgggagcc ggcgccctgc tcgccgtagg
     1621 ccagctccga ggggatgaag agctgccatt tcgccccggt cttcatcagc tgcagcgcct
     1681 ccacccatcc ggggatgatc tggcccacct cgaaggtggc cggctcattg cggcgatagg
     1741 agctgtcgaa ctccgtgccg tcgatcaacg tgccacggta gtgcaccgtc accgtgtcgg
     1801 tcaccttggg cgacttgccc gttccctctt tgaggacctt gtactgcagt ccgctcggga
     1861 gggtaacgac gccaggcttc ttcttgttg
//
LOCUS       DAOSCV010000915         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40798_length_1888_cov_3.250955, whole genome shotgun sequence.
ACCESSION   DAOSCV010000915 DAOSCV010000000
VERSION     DAOSCV010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40798_length_1888_cov_3.250955"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1555
                     /gene="miaB"
                     /locus_tag="PLY56_17650"
     CDS             182..1555
                     /gene="miaB"
                     /locus_tag="PLY56_17650"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ23354.1"
                     /translation="MERTEAAYPELSGKSYYVMTFGCQMNVSDTERMEAILSDAGMER
                     APSAEHADLVLLNTCSVRQKAEDRAYGNLGHLKPLRSARPGMLIGVCGCMAQREGEDV
                     LRKAPHVSLVIGTAQIHRLPGLAATAWRTGERLVATDLPARRSLEEIEAEEDLLAGLA
                     RGRDPHKAWVPVMYGCDRFCAFCIVPMTRGAERSRRPEEIEAQVRAAAAAGRKEVTLL
                     GQTVNSYGHKLPRGITFAELLRRLNEIEGIERIRFTSPYPMGFTDELIDAIAELPKVC
                     EHVHLPLQSGDDEVLAAMRRGYTVAEYEKVYWALRERVPGISITTDLMVGHPGETLEQ
                     HENSLRTVGRLRFDAAFTFHFSPRPRTRAARMPGQVPQAEKMRRLAELIELQNAITME
                     RNASHIGEVLEVLVDGPSDKDPSVLAGYARNNKLVHFPGPAHLAGELCQVRITEAHIW
                     GLSGERA"
BASE COUNT          315 a          622 c          645 g          306 t
ORIGIN      
        1 cagcgcggtg atttatcgcc gcgcgccggg ttgcgcgctg cctccgaggc ttcggtttcc
       61 ttatgtcgga atctcagagg cacagcggca tccattcact ctgttgtcgc gcgggatcct
      121 ttgtggtatg atgggagtgc gggcgaccac gcccggccac cggagacgag cctatctaaa
      181 gatggagaga acggaagcgg catacccgga gctatccggc aagagttact acgtgatgac
      241 gttcggctgc cagatgaacg tctcggacac ggagcggatg gaggcgatcc tctctgatgc
      301 cgggatggag cgcgctccga gcgcggagca cgccgatctg gtgctcctca atacctgctc
      361 ggtccgccag aaggccgagg atcgcgcgta cggcaatctg ggtcacttga agccactccg
      421 cagcgcccgg cccgggatgc tcatcggcgt ctgtggctgc atggcgcagc gggaagggga
      481 ggatgtcctg cgcaaggccc cccacgtgag cctggtgatc ggcaccgcgc agatccaccg
      541 gcttcccggg ctggccgcca cggcgtggcg taccggcgag cggctcgtcg cgaccgactt
      601 gccggcaaga cgcagcctcg aggagattga ggccgaagag gacttgctcg ctggtctcgc
      661 ccggggccgc gacccccaca aggcgtgggt accggtgatg tatgggtgcg accgcttctg
      721 cgctttctgc atcgtgccga tgacccgggg tgccgagcgg agccgccgcc cggaggagat
      781 cgaggcgcag gtgcgtgccg ccgccgctgc cggccgcaag gaggtgacgc tcctcggcca
      841 gaccgtcaac tcctacgggc acaagctccc cagggggatt acctttgccg agctgttgcg
      901 gcgcctgaac gagatcgagg gaatcgagcg gatccgcttc acctcgccct atccgatggg
      961 cttcacggac gagctgatcg atgcgatcgc ggagctgccg aaggtgtgtg agcacgtcca
     1021 cctgccgctc cagtcgggcg atgacgaggt actcgccgcc atgcgccgcg gctacaccgt
     1081 ggccgaatat gagaaggtct attgggcgtt gcgcgagcgc gtgccgggga tcagcatcac
     1141 gacggatctc atggtgggcc atccgggcga gacgctcgag cagcacgaaa actcgctccg
     1201 caccgtcggg cgcctccggt ttgatgccgc ctttacgttc cacttcagcc cgcgcccgag
     1261 gacgcgggcc gcgcgcatgc cgggccaggt gccgcaggcg gagaagatgc gccgtctcgc
     1321 ggagctcatc gagctccaga acgcgatcac aatggagcgc aacgccagcc acatcggcga
     1381 ggtgctggag gtgctggtcg acggtccgag cgacaaggat ccctcggttc tggccggcta
     1441 cgcgcgcaac aacaagctcg tccacttccc tggtccggcc cacctggccg gcgagctctg
     1501 ccaggtgcgg atcacagagg cgcacatctg gggcctctcc ggcgagcgcg cttgagcacg
     1561 acagacgcgc tgtcggcccg cgcgcttgtc gcagccggca ggccccgcgc ggagggatga
     1621 accttgcccg gcgccttacc cccggccagg ggaaggcgaa cacggaaggt tcgcccccac
     1681 gcgcgaccag ttgccgacca ttccgttctg cctgctggtg gttcacctcg atccagcatc
     1741 accgaaacgc cacagtcctt ccggctccat cgatcggtgc ccatctgcgt ccatctgcgg
     1801 attaaagaga gggcccgcct gttcctgaac ggcagcggcg cagcctcttg cgccttgggg
     1861 agcgggagga agagcctgga tgctcggg
//
LOCUS       DAOSCV010000916         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40863_length_1886_cov_3.060623, whole genome shotgun sequence.
ACCESSION   DAOSCV010000916 DAOSCV010000000
VERSION     DAOSCV010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40863_length_1886_cov_3.060623"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PLY56_17655"
     CDS             complement(<1..489)
                     /locus_tag="PLY56_17655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ23355.1"
                     /translation="MSFYDLIERRRSVRRYQETSVSEESVRRVAEAARLAPSACNNQP
                     WHLFVVRDPETRRALFPGAGQAWIAAAPVVLVMCSRPSEAWVRRYDGKNHADIDVAIA
                     MEHVVLAATEEGLGTCWICAFDPQLFRDVLKLPADLEPVAATPLGVPDEAPPARPRKD
                     LEE"
     gene            complement(489..1763)
                     /locus_tag="PLY56_17660"
     CDS             complement(489..1763)
                     /locus_tag="PLY56_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23356.1"
                     /translation="MIEAGVAIRDITPKGTVWMDGYGARDRPSEGVYAPLTARALALR
                     TGETTAAIVVADILNLDRTQEALLRESICTATGLDPEAVILCATHTHCGARVADLMTP
                     GDPDPAYLTHLWGAMRDAVAEALDRLQPAKLTFSRARSCMGINRRLPDGEGGVRFHPN
                     PEGVIDRDLDTFWLTTPDGAPLASVTTYGCHPTSRSGYLLGPDYPGFLRAGIEREWGG
                     TALFLTGCAGNIRPAFLDANGRFRSAEVAEVEAAGEAMAAEALAGRAGTVALDAPSLR
                     VTLGTASLPIAEAPQRERLEALAAGDDPAAAKWARRILEKLAEGPLPERTPFTIQTLR
                     LDARHAFVFLAGEVVTEIGLRIKQTYPYQAITPVAYSNGLVGYVPSRSIHPQGGYEVL
                     GSYRFYYQPAPFTPDVEDVILAEVKRQMEFEC"
BASE COUNT          331 a          644 c          612 g          299 t
ORIGIN      
        1 ctcctcgaga tctttgcgcg ggcgggccgg gggtgcttca tccggcaccc cgagcggcgt
       61 cgccgcgacc ggctccagat ccgcaggcag cttcagcacg tctcggaaaa gctgcgggtc
      121 gaaggcacag atccagcagg tgcccaggcc ctcctcggtg gcggcgagca cgacatgctc
      181 catggcaatc gccacatcga tatcggcgtg gttcttgcca tcgtagcggc gtacccacgc
      241 ctcggacggg cggctgcaca ttacaagcac cacgggcgcg gcggcgatcc atgcctgccc
      301 ggccccgggg aaaagcgccc gccgtgtctc tggatcgcgc acgacgaaaa ggtgccacgg
      361 ctggttgttg caggccgagg gcgccagccg ggccgcctcg gcgacccggc gcacgctctc
      421 ctctgaaacc gacgtctcct gatagcggcg cacgctgcgc cgtcgctcga tcagatcgta
      481 aaagctcatc agcactcgaa ctccatctgc cgcttgactt cggccaggat gacgtcctcc
      541 acatccggcg tgaagggagc gggctggtag tagaagcggt aggaacccag gacctcgtag
      601 cccccttgcg ggtggataga acgcgacggc acgtagccaa ccaggccgtt ggaataagcc
      661 acaggtgtaa tcgcctgata gggataggtc tgtttgatcc gcaacccgat ctcggtcacc
      721 acctcgcccg ccaggaagac gaatgcgtgc cgggcatcca ggcggagtgt ctggatggtg
      781 aagggagtcc gctctggcag cggcccctct gccagctttt cgaggatgcg gcgcgcccac
      841 tttgccgccg cgggatcatc ccccgccgcg agtgcttcca gtctctcccg ctgaggggcc
      901 tcggcgatag gaagcgacgc cgtacccaga gtaacgcgca gggatggagc gtcgagcgca
      961 accgtgccgg cgcgaccagc cagggcctcc gccgccatcg cttcgccagc ggcctccacc
     1021 tcggcgactt cggcgcttcg gaaccgtcca ttggcatcca ggaacgccgg acgaatgttg
     1081 ccggcgcacc cggtgaggaa gagcgcggtg ccgccccatt cccgctcgat cccggcacgc
     1141 aagaagcccg ggtaatccgg gccgaggagg tagcccgagc gcgacgtggg atggcagcca
     1201 tacgtagtca ccgaggcgag tggggcgccg tcgggcgtgg tgagccagaa ggtgtcgaga
     1261 tcgcgatcaa tcaccccctc gggattcgga tggaaccgca cgccgccttc gccatcgggg
     1321 agccggcgat tgatccccat gcaactgcgc gcgcgagaga atgtgagttt cgccggctgg
     1381 aggcgatcca gggcttcggc cacggcatcg cgcatcgcgc cccaaagatg ggtcagatag
     1441 gcagggtctg gatcgccggg cgtcatcaag tcggccaccc gcgccccgca gtgcgtatgc
     1501 gtcgcgcaca gaatgaccgc ctccggatcc aggcccgtcg ccgtgcagat gctctcgcgg
     1561 agaagcgctt cttgcgtccg atccagattg agaatatccg caacaacgat cgcggcggtc
     1621 gtctcccccg tgcgcagggc aagggcacgc gccgtcagag gcgcgtaaac cccctccgag
     1681 ggacggtcac gagcgccgta gccatccatc cagaccgtac ccttgggggt gatatcgcgt
     1741 atcgcgacgc ctgcctcaat cacagctcga tcctccttct tgagaagcac cgctcttcgg
     1801 gcttccgctc aattcggcaa tcgccttcgc cggatgcgcg ccctaccgcg agccgccgta
     1861 cggttgaact agctggccct gggtct
//
LOCUS       DAOSCV010000917         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_40960_length_1883_cov_2.850109, whole genome shotgun sequence.
ACCESSION   DAOSCV010000917 DAOSCV010000000
VERSION     DAOSCV010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40960_length_1883_cov_2.850109"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLY56_17665"
     CDS             complement(<1..765)
                     /locus_tag="PLY56_17665"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ23357.1"
                     /translation="MHPVPPRLDPLASVVAHSPAETRAYLGSPSIVRLPDASLVVSHD
                     FFGPGAPHDHYGREYLTHVYRSADEGAHWERIAEIPGAFWSSLFLHRGALYLLGCTAE
                     YGDIAIRRSEDGGRSWTTPVDERTGLLFPGGPGRENPNYHCAPVPVTLHAGRLWRAFE
                     DCHDARWGSGFLALVISAPEDADLLDARRWRMTNKLPYDKETDRASFDRDGKCGWLEG
                     NVVAGPDGRLWNILRVHSMTPEGHAAMVALDPETHTL"
     gene            complement(826..>1883)
                     /locus_tag="PLY56_17670"
     CDS             complement(826..>1883)
                     /locus_tag="PLY56_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HOJ23358.1"
                     /translation="VTDFEIPKKYGLRQTIGDTLGIGGIFRALRTIPVMLDICRDMEE
                     LCPDAIFLNYTNPMAMLCRAIQSQTKIRSTGLCHSVQGTAAMLAKWIGAPMEEITYLC
                     AGINHQSFYLEFKWKGQDAYPLLRKAIEDPEIYNHEIVRNEMFKHLGYYVTESSGHNS
                     EYNAWFRKRPDLIEKYCTHGTGWNPGVHAYILNYYMSREDTWKEETKAWLAKKEPINL
                     ERGQEYAASIINAVCGDGTIFHFNGNVRNFGLIDNLPEGCCVEVPIYADRRGFNPVHV
                     GPLPPQCAALVNISANNEEMAVEGALSGDPRLVFHSICYDPLTSAVLSLEEIRSMVKE
                     MFEVNRDYLPQFKTIDF"
BASE COUNT          309 a          557 c          665 g          352 t
ORIGIN      
        1 gagcgtgtgc gtttccgggt cgagcgcgac catcgcggcg tggccttcgg gggtcatgct
       61 gtggacgcgc aagatgttcc atagccggcc atcggggccg gcgacgacgt ttccctcgag
      121 ccagccgcac ttgccgtcgc ggtcgaagga ggcgcggtcg gtttctttat cgtaaggcag
      181 cttgttggtc atgcgccatc gtcgggcatc caggaggtct gcatcctcag gcgcggagat
      241 gacgagagcg aggaagccgg agccccatcg ggcatcgtgg caatcctcga acgcgcgcca
      301 gaggcgcccg gcgtggaggg tgaccggcac cggagcacag tggtagttcg ggttttcccg
      361 ccctggcccg ccggggaaga ggaggccggt gcgctcgtct accggcgtgg tccacgagcg
      421 gccgccatcc tcggagcgcc ggatggcgat gtcgccgtat tcggcggtgc atcccaggag
      481 gtagagcgcg ccgcggtgca ggaagagaga ggaccagaat gccccgggga tctcggcaat
      541 ccgctcccag tgtgccccct catctgcgga gcggtagacg tgcgtgaggt actcgcgccc
      601 atagtgatca tggggcgcgc cgggcccgaa gaagtcgtgc gagacaacca ggctggcgtc
      661 cggcaggcgg acgatgctgg ggctccccag gtaggcgcgc gtctccgcgg ggctatgcgc
      721 gacaacgctt gccaggggat cgagccgggg cggaacagga tgcatggcag cccaccttat
      781 cagaaggaat gggaggcgag ggcgcctccc atcccgtagc ggcgcttaga agtcgatggt
      841 cttgaactgc ggcaggtagt cgcggttgac ctcgaacatc tccttgacca tcgagcggat
      901 ctcctccagc gagaggaccg ccgaggtgag cggatcgtag cagatggagt ggaagacgag
      961 gcggggatcg ccggagagcg cgccttcgac ggccatctcc tcgttgttcg cgctgatgtt
     1021 gacgagtgcg gcgcactgcg gcgggagcgg tccgacgtgc accgggttga acccgcgccg
     1081 gtcggcgtag atcggcacct ccacgcagca tccctccggc aggttatcga tcaggccgaa
     1141 gttgcgcacg tttccgttga agtggaagat cgtgccgtcg ccgcacaccg cgttgatgat
     1201 cgaggcggca tactcctgcc cgcgctccag gttgatcggc tccttcttgg ctagccacgc
     1261 cttggtctct tccttccacg tgtcctcgcg cgacatgtag tagttgagga tgtaggcatg
     1321 gacgccggga ttccagccgg ttccgtgggt gcaatacttc tcgatcaaat ctgggcgctt
     1381 gcggaaccag gcgttgtact ccgagttgtg cccgctcgac tcggtgacat agtagccgag
     1441 gtgcttgaac atctcgttgc gcacgatctc gtggttgtag atctcggggt cctcaatcgc
     1501 cttgcggagg agcggatagg cgtcctggcc cttccacttg aactccaggt agaacgactg
     1561 gtgattgatg cctgcgcaga ggtaggtgat ctcctccatc ggggcgccga tccacttggc
     1621 aagcatcgcc gctgtgccct gcacgctgtg gcagaggccg gtgctccgga tcttcgtctg
     1681 gctctggata gcgcggcaga gcatcgccat cgggttcgtg tagttgagga agatggcgtc
     1741 ggggcagagc tcctccatgt cgcggcagat gtcgagcatc accgggatgg tgcgcagggc
     1801 gcggaagatg ccgccgatgc cgagcgtgtc gccaatcgtc tggcgcagcc cgtacttctt
     1861 cgggatctcg aagtcggtga cgg
//
LOCUS       DAOSCV010000918         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41160_length_1876_cov_3.222954, whole genome shotgun sequence.
ACCESSION   DAOSCV010000918 DAOSCV010000000
VERSION     DAOSCV010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41160_length_1876_cov_3.222954"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1876)
                     /locus_tag="PLY56_17675"
     CDS             complement(<1..>1876)
                     /locus_tag="PLY56_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23359.1"
                     /translation="GGFELPRPAVRLETGPTEEELKLQAEIRKRQAEAARTTLESALA
                     AEAEAVKGPARALLDIEREAQRLTTFVDERGVIHRYTLVAEARHNLERELQAKLRALQ
                     KESIEETWKRYREEYEQRLAWETELYQRRLANDEDTARRVLEHTERVYGFEEQRAGWV
                     RDAQLRQAEATDAQTIEQKVALEQRKMEIEVAYLERVNEIKQRLFDMETSRMVLEEEA
                     NLRRLGYRADEIRARIGELTQQREEIRRQQQEAIDAAVQAARENAAIRQTEMIRDHNR
                     QIFESLKRQAEGVFDALLTKSQSVWSAIGNALKTAILTAIKEIVSSRVAALLMQLFSG
                     ARVPIATGVRGSRLGALLGIGAAPVFGGGGPIPGGSAGGWGTPPFVPAGAGWRAMLGG
                     WREFLGSGGSIQLAPGVATTWQAATLWQKLSAIGRSPAAALGGGLLALAGLQRGGLSG
                     LAMTTAGGALVGFKYGGPLGAAIGAGVGAIAGIARLFVKSAEEKAREKIKATYGIDIK
                     DKGVLKQIVEMAKQGFGGNLDMAIRSQQIRDLIQLYAMSTGQSTRGMPATVHPLDIVQ
                     MGGSLYQSPGYSNGTALPGLGGLPTLDTIGGGVASGAGLGTTVVNLQIDSKTVGNVII
                     Q"
BASE COUNT          278 a          665 c          593 g          340 t
ORIGIN      
        1 tctggatgat cacgttccca accgtcttcg agtcgatctg gagattgacc accgtcgtgc
       61 cgagcccggc tccggatgcc acaccgccac cgatggtatc gagcgtgggc agcccgccca
      121 ggccggggag cgctgtgccg ttcgagtacc ccggcgactg gtacaatgac ccgcccatct
      181 gaacgatgtc gagcgggtga accgtggcgg gcatgcctcg agtcgactgc cctgtgctca
      241 tcgcgtacaa ctggatgaga tcgcggatct gctggctgcg aatcgccatg tcgaggttcc
      301 cgccgaaccc ctgcttcgcc atctccacga tctgcttcag gacgcccttg tccttgatat
      361 cgatgccgta ggtggccttt atcttctcgc gagccttctc ctcggcgctc ttgacgaaca
      421 ggcgagcgat gcccgcgatg gcgccaacac cagcgccgat cgctgcaccc agcggcccgc
      481 catacttgaa gccgacgagc gcgccacctg ccgtggtcat cgcgaggccc gaaaggcccc
      541 cgcgctggag gccggccagg gcgagtaacc cgccacccag ggccgcggcg ggcgaacggc
      601 caatggcgga gagtttctgc cagagtgtgg ccgcctgcca ggtggtggcg acgcccggcg
      661 caagctggat cgagccgccg ctgcccagga actcgcgcca gccgccgagc attgctcgcc
      721 agcctgcgcc cgccggcacg aacggtggcg tcccccaacc gccagccgag ccgcctggaa
      781 ttggcccgcc tccgccaaag accggtgcgg caccaatgcc gagcagggcg ccgagccgac
      841 tgccgcgaac tcccgtggcg atgggaacgc gcgccccgct gaacaactgc atcagcaggg
      901 cggccacgcg cgaggagacg atctctttga tagccgtcag gatcgccgtc ttgagcgcgt
      961 tgccgatggc cgaccagacc gactgggatt tcgtcagcag cgcatcgaag acgccttcag
     1021 cctggcgttt gagcgattcg aagatctgcc gattgtgatc ccggatcatc tcggtctggc
     1081 ggatggcagc gttctcacgc gccgcctgga cggcggcgtc aatggcctcc tgttgctgcc
     1141 gccggatttc ctcgcgctgc tgggtcagtt ccccgatccg cgccctgatc tcgtctgcgc
     1201 ggtagcccaa gcgccggagg ttggcctcct cttcgagcac catgcgcgag gtctccatat
     1261 caaacagtcg ctgcttgatc tcgttgaccc gttccaggta ggcgacctcg atctccatct
     1321 tgcgctgctc gagggcgacc ttctgctcga tggtctgtgc atcggtcgcc tcggcctggc
     1381 gcaactgggc gtcgcgcacc cagccggcac gctgctcctc gaagccgtag acccgctcgg
     1441 tgtgctcgag cacccggcgc gcggtgtcct catcgttggc caggcgacgc tggtaaagct
     1501 ccgtctccca ggcgagccgc tgctcgtact cctcgcggta gcgcttccag gtctcctcga
     1561 tactctcctt ctggagcgca cgcagcttgg cctggagttc gcgctcgagg ttatggcggg
     1621 cctcggcgac gagcgtgtag cggtggatca ccccgcgctc atcgacgaac gtggtcaacc
     1681 gctgagcctc gcgctcgatg tccaagagcg cacgcgccgg ccctttgacc gcttccgcct
     1741 ctgcggccag ggcgctttca agcgtcgtgc gcgcggcttc ggcctggcgc ttgcggatct
     1801 cagcctggag tttcagttcc tcctcggtcg gcccggtctc gagcctgacg gcgggcctcg
     1861 gaagctcgaa gcctcc
//
LOCUS       DAOSCV010000919         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41197_length_1875_cov_3.420879, whole genome shotgun sequence.
ACCESSION   DAOSCV010000919 DAOSCV010000000
VERSION     DAOSCV010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41197_length_1875_cov_3.420879"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLY56_17680"
     CDS             complement(<1..389)
                     /locus_tag="PLY56_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOJ23360.1"
                     /translation="MNAFDVAGITGLTERDAAERLERDGPNELPTARARGLATVVWEV
                     AQEPMFLLLVAAGIVYLALGSLPEAGMLLGFVMVVMGITIYQERKTERALSALRELSS
                     PRALVIRDGEHQRIAGRDVVRGDVMIL"
     gene            complement(426..821)
                     /locus_tag="PLY56_17685"
     CDS             complement(426..821)
                     /locus_tag="PLY56_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ23361.1"
                     /translation="MAVKVYGINHIALEVTDVSEAVRFYREVFDLPEPDAGEGQAFFR
                     MGEHQFLALFKVDRKQPDDHRHFGIIVTDDDEIARVRERVQARGLALTPGFACDFRDP
                     WGNRIQVVDLSVSSPYWRLGRVKMSEESA"
     gene            950..>1875
                     /locus_tag="PLY56_17690"
     CDS             950..>1875
                     /locus_tag="PLY56_17690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOJ23362.1"
                     /translation="MAFTLEINRLRQEVERALPVATEIRRDLHRHPELALEERRTAQV
                     VLTRLQKLGVEALRVDETGALGLIQGGRPGPVVALRADMDALPVTEETGVPYASQHAG
                     KMHACGHDGHTACLLGVAEVLSRLRQELPGTVKLIFQPAEEGGGGARRMVEAGVLQDP
                     DVERIVALHSWPDLLAGQIGVRAGAMLAAVDAFQVTVRGRGGHGAHPHRCIDPILISA
                     RIIDAVQSIVSREVDPLESAVVTVGHIEGGKTRNVIPDTCFFEGTVRTLSEETRRHVQ
                     SVFMRTVNGVAAAHGATAEIAYHRELPVVINDV"
BASE COUNT          327 a          610 c          640 g          298 t
ORIGIN      
        1 ctgagaatca tcacgtcgcc gcggacgaca tcgcggccgg cgatgcgctg gtgctcgcca
       61 tcgcggatga caagggcgcg cgggctggag agctcgcgga gcgccgagag ggcgcgctcc
      121 gtctttcgct cctggtagat ggtgatcccc atcaccacca tgacgaagcc gaggagcatt
      181 cccgcctcgg ggaggcttcc caaggccagg tagacgatgc ctgccgccac caggagcagg
      241 aacatcggct cctgtgccac ctcccagaca acggttgcca acccgcgcgc cctcgccgtg
      301 ggcaactcgt tggggccgtc gcgctcgagc cgttccgcgg catcccgttc tgtgagcccg
      361 gtgatccctg cgacatcgaa agcgttcact cgcgcggatg atgcaggcgc cgtgccagtg
      421 ccacgctacg cgctctcttc cgacatcttc acccggccca ggcgccagta gggcgaggag
      481 acagagagat cgacgacctg aatgcgattg ccccaggggt cgcgaaagtc gcaggcgaaa
      541 cccggagtca gcgcgagacc gcgtgcctga acgcgctcgc gcacgcgcgc gatctcgtca
      601 tcgtcggtga cgatgatccc gaagtggcgg tgatcatccg gctgcttgcg gtccaccttg
      661 aaaagagcga ggaactggtg ctcgcccatc cggaagaagg cctgtccctc tcccgcgtcc
      721 ggctccggca ggtcgaacac ctcccggtag aagcgcactg cctcgctgac gtccgtaacc
      781 tccagagcga tgtggttgat gccatacacc ttcactgcca tgagcgtcct cctatcgcaa
      841 cgttctcata ggcgatccca cagaggatgc ttagcatgct tgatgccgga cacaagcgcc
      901 aggaggacgc aggcgaatgg ggaaagtgag gaagggagga tgatggccaa tggcatttac
      961 tttggagatc aaccggctgc gacaggaagt ggagagagcg ctgccggtgg caaccgagat
     1021 ccgccgggat ctgcaccggc atccagagct ggcgctcgaa gagcggcgaa cggcgcaggt
     1081 ggtgctcacg cggctgcaga agctaggcgt tgaggcgctg cgcgtggatg agaccggggc
     1141 gctcggcctc attcaggggg ggcgcccggg gcccgtggtg gctctccgcg cggatatgga
     1201 cgccctcccg gtcacagagg agacgggcgt gccatatgcc tcgcagcacg cgggcaagat
     1261 gcacgcctgc gggcatgacg ggcacaccgc ctgccttctg ggtgttgccg aggtcctctc
     1321 tcgcctgcgc caggagcttc caggaacggt caagttgatc ttccagcccg ccgaggaggg
     1381 cggcgggggc gcccggcgga tggtcgaggc cggtgtactc caggatcccg acgtcgagcg
     1441 gattgtcgct ttgcactcgt ggcccgacct cctggccggt cagatcggcg tgcgtgccgg
     1501 cgcgatgctc gccgccgtcg acgccttcca ggtgacggtc cggggtcgtg gcgggcatgg
     1561 ggctcacccc caccggtgca tcgacccgat cctgatctcg gcccggatca tcgacgcggt
     1621 gcagagcatc gtcagccgcg aagtcgaccc cctggaatcg gccgtggtca ctgtggggca
     1681 tatcgaaggc ggcaagacgc gcaacgtcat cccggacacg tgcttcttcg aaggaacggt
     1741 gcgcaccctg agcgaggaga cgcgccggca cgtgcagtcg gtcttcatgc gcaccgtgaa
     1801 cggtgtggcc gcggcccacg gggcgaccgc cgagatcgcc taccatcgcg agctgcccgt
     1861 ggtgatcaac gacgt
//
LOCUS       DAOSCV010000920         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41233_length_1874_cov_3.543705, whole genome shotgun sequence.
ACCESSION   DAOSCV010000920 DAOSCV010000000
VERSION     DAOSCV010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41233_length_1874_cov_3.543705"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1221
                     /locus_tag="PLY56_17695"
     CDS             346..1221
                     /locus_tag="PLY56_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ23363.1"
                     /translation="MRREVRWERMFPDELEAAFQECPVVYLPYGLCEPHGPQSALGMD
                     GLRAHAYACHAAREHGGIVAPPFFWNVHELGIYAAWAHPLIGEATPWLTAVPPWLFFH
                     EICYHVRSMDALGFKAILLFSGHDGPHGRDLKRLENLLQPHFAARLCFLTDASALRLE
                     GFHHGGKIETSLLWAAEPDCVDTSRFPAPDDPGPHFAMGPDAREADRRLGEELIARFA
                     SALAAKAKELLEAYDRLRPQRRPLTFEETERIWQEEIRPRMGEMASMCDTRPGQAPPP
                     PESRWHHNWKVPKLD"
     gene            1232..>1874
                     /locus_tag="PLY56_17700"
     CDS             1232..>1874
                     /locus_tag="PLY56_17700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ23364.1"
                     /translation="MLQPKLGASLHTIEKNLTPEALAAVEGSRIATVEIHPILFAADA
                     EGSMRAALKEMLARTGIRPMTIHAPFGGAYDLSSLDERTRQAGLEVVLEAVDLAVEFN
                     APMVVVHPSAEPIPAEEREERLTRSRQSLARVGERCAETGRRVALELLPRTCLGNTIE
                     EIFALLEGLPEEQFGVCLDVNHLMDRFAELPEAVRRLGSRLITTHLSDYDGVDE"
BASE COUNT          318 a          618 c          624 g          314 t
ORIGIN      
        1 caatgatcgc tatgacgacg agcaattcga tgagcgtaaa tcccttctgc ttcatacggt
       61 tccctcctct tcctgcgcgg ggtatagcac ccccctggta cggagactgt ctgtgcctca
      121 cgccatcatt atagcacacg agcgcaagga tacgatcagg gttcggtggc cattcgcaga
      181 gattccatcg aaaggagaga cacgccctcg ctccgggtga cacgggaagt gccggcgagc
      241 gcaaaggggg gatggacgcc tcgcgtgccg aatgctacct tgcggctcat cggggctgcc
      301 tgctccgcca ggagaccagg agcgggcgtg aataggagga tttgaatgag gcgagaggtg
      361 cgatgggagc ggatgttccc ggacgagctg gaagcggcgt ttcaggagtg cccggtggtc
      421 tacctgcctt atggcctctg cgagccgcac ggcccgcaga gcgctctcgg catggacggg
      481 ctgcgagctc acgcctacgc ctgccacgcc gcgcgggagc acggtgggat cgtggcgccc
      541 cccttcttct ggaacgttca tgagctcggg atctacgcgg cctgggccca cccgctcatc
      601 ggggaggcaa cgccctggct caccgcagtg cccccctggc ttttcttcca tgagatctgc
      661 taccacgtgc gctccatgga tgccctcggc ttcaaggcga ttctcctctt ctccgggcac
      721 gacggccccc acggtcgcga cctcaagcgg ctggagaacc tcctccagcc ccacttcgcc
      781 gcgcgcctct gcttcctgac cgacgcgtcg gcgctccggc tggagggatt ccatcacggc
      841 ggcaagatcg aaacctcgct gctgtgggcg gcggagccgg attgcgtcga cacgtcgcgc
      901 ttcccggcgc ccgatgaccc cggcccgcac ttcgcgatgg gcccggatgc gcgggaggcc
      961 gaccgccgcc tgggcgagga gctgatcgcc cgcttcgcca gtgcgctcgc cgcgaaagcg
     1021 aaggagctgc tggaggcgta tgaccgcctg cggccacagc gccggccgct cacttttgaa
     1081 gagaccgagc ggatctggca ggaggagatt cgcccgcgaa tgggcgagat ggcctcgatg
     1141 tgcgacacgc gccccgggca ggcgccgccc ccgccggaat cgcgctggca tcataactgg
     1201 aaagtgccca agctggacta ggagtgagag tatgctgcaa ccgaagctcg gagcttcgct
     1261 ccacaccata gagaagaacc tgacgcccga ggcgcttgcc gcggtcgagg gctcgcggat
     1321 cgccacggtg gagatccacc cgatcctttt cgcggcggac gcggaaggct cgatgcgtgc
     1381 cgccctgaag gagatgctcg cacgcacggg gatccgtccg atgacgatcc atgcgccgtt
     1441 tggtggggcg tatgacctct ccagcctcga cgagaggacg agacaggccg gcctggaggt
     1501 ggttctcgag gccgtcgacc tggcggtaga gttcaacgcg ccgatggtcg tcgtccaccc
     1561 tagcgccgag ccgatcccgg cggaggagcg cgaggagcgc ctcacacgct cacggcaatc
     1621 tctagcccgg gtaggcgagc ggtgcgccga aaccggccgg cgggttgcgc tagagcttct
     1681 cccgcgcacc tgcctcggca acaccatcga ggagattttc gccttgctgg aaggcctccc
     1741 cgaggagcag ttcggggtct gcctggatgt gaaccatctg atggaccgct tcgcggagct
     1801 gccggaggcc gtccggcgcc ttggctcgcg gctcatcacc acgcaccttt ccgactatga
     1861 cggcgtggat gaaa
//
LOCUS       DAOSCV010000921         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41472_length_1867_cov_2.827815, whole genome shotgun sequence.
ACCESSION   DAOSCV010000921 DAOSCV010000000
VERSION     DAOSCV010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41472_length_1867_cov_2.827815"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PLY56_17705"
     CDS             complement(<1..718)
                     /locus_tag="PLY56_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23365.1"
                     /translation="MTELSVLAVAVALLGIVVGLRAADRVRHRRSYQRLLGKRAAFTM
                     TPARWAAVGLTLLVVAILSSGGPGPRRNASRPDAVAVTPPGEAKPAPPADAPDSKNSQ
                     AAQSQPAPSNSTPEATDHASPTVPSDLAEAAAEEQRWCNQGDRMMAIGQYSMAAQAYW
                     EALRIDPSSQRARAGLTRARRAEAEAEAARQQESLFPMETPSATEIPTERPVREASSA
                     LKRPARETPPTDDSRWGPQED"
     gene            1029..>1867
                     /locus_tag="PLY56_17710"
     CDS             1029..>1867
                     /locus_tag="PLY56_17710"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOJ23366.1"
                     /translation="MARRSRRPESTTANSGAKEIIVNVSPREDRVAIIENGQLAELYI
                     EREERVVGSIYKGKVVNVLPGMDAAFVDIGLEKNAFLYVGDILPSSEEEAEAVATEME
                     AGAAPPPPPPRRNGRRTGIKDVAKVGQQILVQVVKAPRGTKGARVSTRISLPGRYLVL
                     MPDTDHVGVSRKIEDEAERERLKELARQVKPQGMGLIVRTMAEGAEKEELKEDLDALL
                     QEWSRIQRKFRSKGSGPLIYRDHDLIYRILRDLYDPEQTEIIVDTKALQNRVQRELDD
                     LDLP"
BASE COUNT          348 a          566 c          608 g          345 t
ORIGIN      
        1 catcttcttg cgggccccac cggctgtcat cagtcggtgg tgtctcccgc gcaggccgtt
       61 tcagcgccga cgatgcttcc cgcacgggcc gttcggtggg gatctccgtc gcggacgggg
      121 tttccatggg gaacaggctc tcctgttggc gcgccgcttc ggcttcggcc tcggctctgc
      181 gggcccgcgt caggccagca cgcgcgcgct gcgaggaggg gtcgatccga agcgcctccc
      241 agtaggcctg cgccgccatg ctatactggc ctatcgccat catgcggtct ccctggttgc
      301 accaccgctg ctcctcggca gccgcttcgg ccaggtcgga aggcactgtt gggctcgcgt
      361 gatccgtggc ctcgggtgta gaattggaag gcgcaggttg cgattgggcc gcctggctgt
      421 tcttcgaatc gggggcatcc gcgggcggtg cgggcttggc ctcgcccggg ggagtgaccg
      481 ctaccgcatc gggacgcgag gcgttccggc gcggccccgg cccgccggac gagaggatcg
      541 cgaccacgag gagggtcaga cccacggccg cccagcgtgc cggcgtcatg gtgaaagccg
      601 cccgcttgcc caaaagacgc tggtaagatc gcctgtggcg caccctatca gcggcgcgca
      661 ggcccaccac aatgccgaga agcgccacag ctacggccag aacagataac tcggtcacca
      721 ccaaaccctc tcccgtgagg cgacagcagg gaggctcccc cactacccac cctattatag
      781 cagcgggcac cgtctcgtgc aaatgggtgg cacatcggcg tgcactatgc attgacattg
      841 gctcttgcgc tatggtacaa tctgcgcgta gcatactgcc ggagggcgtc tggcttggcg
      901 cacaatttgt actgttcaac ctgcctgatt actgtttgtt tggctggtct gtgcctgcgg
      961 catgcgtgcc gcgggctttt gtttttggcc gacacccctg ttctctagat ttcaggaaag
     1021 gactccgtat ggctagacga agcaggaggc cggaatccac gactgcgaac tcaggcgcga
     1081 aggagatcat cgttaatgta agtcctcgtg aggaccgtgt cgcgatcatc gagaacggcc
     1141 aacttgctga gctttacatt gaacgtgaag aacgcgttgt gggcagcatc tataagggca
     1201 aggtcgtcaa tgtcctgcca gggatggatg ctgccttcgt ggatatcggc ctggagaaga
     1261 acgcgttcct gtatgtcggg gatatcctcc ccagcagcga agaggaggca gaagcggtcg
     1321 ccactgagat ggaagccggg gccgcgccac ctccgccacc accccggcgc aacgggcgcc
     1381 gaacgggcat caaagatgtc gcgaaggtgg ggcagcagat cctggttcaa gtagtcaaag
     1441 caccgcgcgg caccaaaggc gcgcgcgtct ccacccggat ctcgctgccc ggacgttatc
     1501 tggtgctgat gcccgatacc gaccacgtcg gggtgtcgcg caagattgag gatgaagccg
     1561 aacgggagcg gctcaaggag ttggcgcggc aggtgaagcc gcagggcatg ggcctgatcg
     1621 tgcgcaccat ggccgaaggg gcggaaaagg aagagctcaa ggaagatctg gatgcccttc
     1681 tccaagagtg gtcgcgcatc cagcgcaagt tcagatccaa gggcagtggc ccgctgattt
     1741 atcgagacca tgatcttatc taccgcatcc tgcgggatct ctacgatccg gagcagacgg
     1801 agatcattgt tgataccaag gctctgcaga accgagtcca gcgggagctg gatgatctag
     1861 atctccc
//
LOCUS       DAOSCV010000922         1866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41509_length_1866_cov_2.862507, whole genome shotgun sequence.
ACCESSION   DAOSCV010000922 DAOSCV010000000
VERSION     DAOSCV010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41509_length_1866_cov_2.862507"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1866
                     /locus_tag="PLY56_17715"
     CDS             17..>1866
                     /locus_tag="PLY56_17715"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOJ23367.1"
                     /translation="MRDLSIIWRMMLLLAVCEAVQAAPAAEIQPWQKLYTGQEATGAN
                     VIALWQFQPGQEAKDASGHGHDLTLRGNSRFVAGGPLGGYLESFLGGTDNDTKMGAVA
                     KDHPDLSPAGAFTLEAWFAAKPEMEQTANVFMIDKKYFNYSREDPRANWDYCLCLARA
                     GKQRRLLAHLGYGKDSATYSSEAFEVKPGEWIHVAFTYDGAGTGRFFVNGQPAGKTVH
                     EGRGPITPGNYDLVLGDRYGSTHSGFPGYLAQVRLSNGIVPYFTGPLEVTPGVGRTAF
                     VRMEKDASFSLTVSNDTDKPLTNGQVQVVFGGNTRRITLPNLAPNQEQVVPIPVDTSL
                     RPDSYPLTVTATAVADGREYRTEKEFQITIVPRPLPDQMPVVMWGTGDIPRLKQIGFT
                     HRLVYLTDYGKIWEAGEPTEAMTTGAIAERARMLDELLVNGLGAAVYIYPGRWVMNNK
                     KLQEQFQRVERTGKPRGNKDVCASFPEIQQYAYNAGASVARTFGHFPALQAALVHSEI
                     RDGTDLCFHPHDQEAYRRETGLEIPAEVQSKSGVRFSSVRGFPARQVVPDDHPLLQFY
                     RWFWKDGDGWNSLHTQVHRGLKSTGRKDLWTFFDPAVRVPSLWGSGGQVD"
BASE COUNT          336 a          602 c          603 g          325 t
ORIGIN      
        1 ggagaatggt gcagtcatga gggatttatc catcatctgg cgcatgatgt tgctgcttgc
       61 ggtgtgcgag gccgtccagg ccgcgccagc cgctgagatc cagccctggc agaagctcta
      121 caccggccag gaagccactg gagcgaacgt catcgccctc tggcagttcc agccagggca
      181 agaggccaag gatgcttccg ggcacggcca tgacctgacg ctccggggca atagccgctt
      241 cgttgccggc gggcccctgg gcggctatct ggagtcgttc ctcggcggca ccgacaacga
      301 caccaagatg ggcgccgtag cgaaagatca cccggatctc tcgcctgccg gcgcgttcac
      361 cctggaggcg tggtttgctg cgaagccgga gatggagcag acagccaacg tcttcatgat
      421 cgataagaag tacttcaact actcccgcga agacccccgg gcgaactggg actactgtct
      481 ctgcctcgcg cgcgcgggga agcagcgccg tctgctcgct cacctgggct atggcaagga
      541 ttcggcgacc tactcctccg aagcctttga agtgaagccc ggcgaatgga tccatgtggc
      601 gttcacttac gacggcgcgg gcaccgggcg cttcttcgtc aatggccagc ctgcgggtaa
      661 gacggtccat gagggccgcg gccccatcac tcctggtaac tatgacctgg tgttgggcga
      721 ccgctatggg agcacgcact ccggcttccc gggctacctg gcccaggtgc gcctcagcaa
      781 tggcatcgtg ccttacttca cgggacccct ggaggtgacc cccggagttg ggcgcactgc
      841 gtttgtgcgc atggagaagg acgcgtcctt ctcgctcacc gtgagcaacg acaccgacaa
      901 gccgctcaca aacggccagg tgcaggtcgt cttcggtggc aatacgcggc ggatcaccct
      961 gccgaacctg gcccctaacc aggagcaggt ggtccccatc cctgtggata cctccctgcg
     1021 cccggatagc tatccgctca cagtgacggc tacggcggtc gctgacgggc gcgagtatcg
     1081 cacggagaag gagtttcaga tcacgattgt gccgcgcccg ctgccggatc agatgccggt
     1141 ggtgatgtgg ggcacgggtg atatcccccg gttgaagcag atcggcttca cgcaccgtct
     1201 ggtttacctc accgactatg gcaagatctg ggaggccggc gagccgaccg aagcaatgac
     1261 caccggcgcc atcgcagagc gtgcgcgcat gctggacgag cttctggtga acggcctggg
     1321 cgccgccgtc tacatctacc cgggccgctg ggtgatgaac aacaagaagc tgcaggagca
     1381 gttccagcgc gttgagcgca cgggcaagcc tcggggcaac aaggacgtct gcgccagctt
     1441 cccggagatc cagcaatatg cctacaacgc cggcgcgtcc gtcgcccgga ccttcgggca
     1501 tttccccgcg ctgcaagcgg cgttggtcca ctccgagatc cgggatggca ccgatctctg
     1561 cttccacccg catgaccagg aggcctatcg gcgggagacc ggcctcgaga tccccgcgga
     1621 ggtgcagtcc aagagcggag tccgcttctc aagcgtccgt ggcttccccg cgcgccaggt
     1681 cgtgccggat gaccacccgc tcctgcagtt ctatcgctgg ttctggaagg acggcgatgg
     1741 ctggaactcc ctgcacaccc aggtgcatcg gggcctgaaa tccaccggca ggaaggatct
     1801 ctggaccttc ttcgatccgg cggtgcgcgt gccgagcctc tgggggagcg gcgggcaggt
     1861 ggatta
//
LOCUS       DAOSCV010000923         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41661_length_1861_cov_3.258029, whole genome shotgun sequence.
ACCESSION   DAOSCV010000923 DAOSCV010000000
VERSION     DAOSCV010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41661_length_1861_cov_3.258029"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="PLY56_17720"
     CDS             complement(<1..1057)
                     /locus_tag="PLY56_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23368.1"
                     /translation="MPEETLRAGASLSNITPALGSALSGSFHPRYAEDVEDDLHARAL
                     VLDNGEKRLALVVCDLICLPRKAVDAAKARIAERCGIPPEAVMISCTHTHSGPATCGL
                     LGTDEVPGYLDFAVPRIADAVERACRRLAPARVAWGAGCEPGLVFNRRYRMKDGSVRM
                     NPGYLHPDLVEPVGPTDPEVGALFVETLEGRPIAVLANYALHYVGGAPGTSVSADYFA
                     RFGQALQRMRGESFVVLLGNGCCGDVNNCNYRGPAPTPAYRWERADRAANLLAAEVMK
                     VWQRCSFRQEVALDCRLEEVTAAVRQPDAETLAKARAMPPKPVSDMDRDELYLREALI
                     MTQEPPEVRTPVQALRVG"
     gene            complement(1176..>1861)
                     /locus_tag="PLY56_17725"
     CDS             complement(1176..>1861)
                     /locus_tag="PLY56_17725"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ23369.1"
                     /translation="RFCPGAHAELMAVPDYACVPIPEDLSFAAGALLAGDGLGVPYHT
                     SRRLPTRGGDTVAVVGCGPIGLGNVMVQAFLGARVIALDRVPDRLRLARDLGAAETVD
                     VSAADPVECVRDLTRGRLADAVIEASGRPEGFALALKIVGKAGTVACNGENTQVSLHV
                     GTDLIRRDITLFGSWFYHIREYPEMVDLVRRGLAVEKLISARYPLEEAQAAYAAFARG
                     ETAKVILEP"
BASE COUNT          301 a          684 c          612 g          264 t
ORIGIN      
        1 cgccgacgcg gagcgcctgg accggcgtcc ggacctcggg cggctcctgc gtcatgatga
       61 gcgcctcgcg caggtagagc tcgtctcggt ccatatccga caccggcttg ggaggcatcg
      121 cgcgcgcctt cgccaaagtt tcggcgtctg gctggcgcac tgcggcggtc acttcctcca
      181 ggcggcaatc cagcgccact tcctggcgga aggagcagcg ttgccacacc ttcatcacct
      241 cggcggcaag caggttggcc gcgcggtcgg cacgctccca gcgatacgcc ggggttggcg
      301 ccggaccgcg gtagttgcag ttgttgacgt cgccgcagca accgttgccc agcaagacga
      361 cgaacgattc cccgcgcatg cgctgcagcg cctgcccgaa gcgcgcgaag taatcggccg
      421 agacgctcgt gccgggcgcg ccgcccacgt agtggagggc atagttggcc agcacggcga
      481 tgggccggcc ctcgagcgtc tccacgaaga gcgcacccac ctccggatcg gttggtccga
      541 ctggctccac cagatcagga tgcaggtagc cggggttcat gcggacgctg ccgtccttca
      601 tccggtagcg acggttgaag acgagacccg gctcgcatcc ggcgccccag gccacccggg
      661 ccggagcgag gcgcctgcac gcacgctcca ccgcgtcggc gatccggggc acggcgaaat
      721 cgaggtagcc gggcacctcg tccgtgccca gcaggccgca cgtggccggg cccgagtggg
      781 tgtgcgtgca cgagatcatc accgcctccg gcgggatccc gcaccgctcg gcgatccgcg
      841 ccttcgcagc atccacggct ttgcgcggca ggcagatcag gtcgcacacc accagcgcca
      901 gccgcttctc gccgttatcg aggaccagcg cgcgggcgtg caggtcgtcc tcaacatcct
      961 cggcatagcg cgggtggaac gagccgctga gggcgctccc cagggccggc gtgatgttgc
     1021 tcagggaagc tcccgcacga agggtttctt caggcatcag atgctccttc atccatgaac
     1081 gcaaacagcg acaaccggga gcaggaggcc aggcgccggt gcgccgccag ccgctcccct
     1141 gctccagcgc caaggcgctc gagcctcgcc attgctcacg gctccaagat cactttcgcg
     1201 gtttcgcccc gggcgaaagc ggcgtaggcc gcctgcgcct cctccagggg ataccgcgcc
     1261 gagatcagtt tctcgacggc aagaccgcgg cgcaccagat cgaccatctc cggatactcg
     1321 cggatgtggt agaaccagct tccgaagagc gtgatgtcgc gccggatcag atcggtgccc
     1381 acatgcaggc tcacctgcgt gttctcaccg ttgcacgcca cggtgcccgc cttgcccaca
     1441 atcttcagcg ccagggcgaa gccctccggc cggccgctcg cctcgatcac cgcgtccgcc
     1501 aggcgccctc gcgtcaggtc gcgcacgcac tccaccgggt cggcggcgct gacatccacc
     1561 gtctcggccg cgcccaggtc gcgcgccaga cggaggcggt ctggaacgcg gtcgagggca
     1621 atcacgcgcg cgcccaggaa cgcctgcacc atcacgttgc ccaatccgat ggggccgcac
     1681 ccgaccaccg cgacggtatc gcccccacga gtgggcaatc ggcggctggt gtggtagggc
     1741 acacccaacc cgtcgcccgc gagaagcgcg cccgctgcga aggagagatc ctccgggatg
     1801 ggcacgcacg cgtagtccgg caccgccatc agctcggcgt gcgcacccgg gcagaaccgc
     1861 t
//
LOCUS       DAOSCV010000924         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_41991_length_1851_cov_3.408129, whole genome shotgun sequence.
ACCESSION   DAOSCV010000924 DAOSCV010000000
VERSION     DAOSCV010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41991_length_1851_cov_3.408129"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..>1851)
                     /locus_tag="PLY56_17730"
     CDS             complement(180..>1851)
                     /locus_tag="PLY56_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23370.1"
                     /translation="LGAQAPSRRLVTLDFVNADIQDVLKALAIQSGANILIAPKVEGK
                     VTVGLRNVTPEQALEYIVRLSSLAYGREDDTYVVCTRDQIKDVFPPGTRGEILFPPPV
                     AEASPGVAVGQPAGPPKVSVVRLERLEAAKAVPLVQKLAPAVQIETPDPKLAVLIGDD
                     AQVAAAEMAIRTLESSLPPLPARPEGVPPKLSEEFEIEVYSLRHVNAYDAGRLLKGFM
                     TSRILPEVVVVPAPAPAYPAPEDDKGRAPIGQRLGDTDDALLNLLAGKNVASGSEAAG
                     KTPPGAPEATEKAGTVLIPEGSTTNTLLLIGARPDIAKVKDYLDRIDVAPKQVMIDVK
                     VTDVSLSDAEQLGITWNWTTLGIQEIAPEGSETIPRMQFGRFGHAPMVFNATLEALLK
                     TNKARLLANPRVAVLDGRTASIHVGDTVRYLAQRTSGINGTTVTIGEESVGVVVSVSP
                     RVGGDDVIVLDVKPKVNVITGFTPTGDGGQVPNTSERSVETVVRLRDGETLAIGGLIR
                     DDDTVTMQKVPGLGDLPILGELFKYRNRQRSNSEVLIFITPTIVPDIS"
BASE COUNT          338 a          639 c          547 g          327 t
ORIGIN      
        1 gaccgctctt accccaaggg tgtgcccccc aaacaggcag tagcacgcct cccggataga
       61 ctcccccgca cctcctctgc ccgggagccg tctctccaga aggcgcagaa ggcagaacga
      121 cgccaccaag agtgcctggt gacgccgctc tggcccaaca tcgcgtccgt cagttgcgct
      181 caagagatat ccggcacaat cgttggagtg atgaagatga gcacctccga gttggaccgc
      241 tgacggttgc gatacttgaa cagctcgccc aagatcggga gatctcccaa tccgggcacc
      301 ttctgcatcg tcaccgtgtc atcatcacgg atcaacccgc caatcgccag cgtttcgcca
      361 tcgcgaaggc gcacaaccgt ctctaccgat cgctcggacg tattcggcac ctgcccgcca
      421 tcccccgtgg gggtgaaacc agtgatcacg ttcaccttcg gcttgacatc gagcacgatc
      481 acgtcgtcgc ccccgacgcg cggcgaaacg ctgaccacca cgccgacgct ctcctcgcca
      541 atcgtcaccg tcgtcccgtt gatgccgctg gtccgttgtg ccagatagcg aacggtatcg
      601 cccacgtgaa tggatgccgt gcgcccatcc agcaccgcca cgcgcgggtt ggccaggaga
      661 cgcgccttgt tggtcttcaa cagcgcttcc agggtggcgt tgaacaccat gggagcgtgt
      721 ccaaagcgcc cgaactgcat cctcgggatc gtctctgatc cctccggcgc aatctcttga
      781 atgcctagcg tagtccagtt ccatgtgatc ccgagctgct ccgcatccga gagcgagaca
      841 tcggtcacct tgacgtcgat catcacctgc ttgggcgcca catcgatgcg gtccaggtaa
      901 tcctttacct tggcgatatc cggacgagcg ccgatgagga gcagcgtgtt ggtcgtgctt
      961 ccctcgggga tcagcacggt acccgccttc tcggtagcct caggcgcgcc cggaggcgtc
     1021 ttccccgcgg cctccgaacc agatgccacg ttctttccgg ccagcaggtt cagcaaagcg
     1081 tcatcggtat cgcccaaccg ctgccctatt ggcgcgcggc ccttatcatc ctcgggagcc
     1141 ggataggcgg gcgccggcgc gggcaccacc acaacctcgg gcaggatgcg agaggtcatg
     1201 aagcccttca acaagcgtcc ggcatcgtag gcattcacat gcctcaacga gtagacctcg
     1261 atctcgaact cttccgacag cttaggtggc acgccctcgg ggcgcgcggg cagcggagga
     1321 agcgaggatt cgagcgtacg gatcgccatc tcggcggccg ccacctgggc gtcatcgccg
     1381 atgagcacgg ccagcttcgg atccggcgtc tcaatctgga ccgcgggggc cagcttctgg
     1441 accagcggca cggccttcgc ggcctcgagc ctctccaggc gaaccacgct caccttcggg
     1501 gggccggcag gctggcccac agcaactccg ggcgatgcct cggcgactgg cggcggaaag
     1561 aggatctcgc cccgcgtgcc cggcgggaag acatccttga tctggtcgcg cgtgcagacg
     1621 acgtaggtat cgtcttcacg cccataggcg agcgagctga gacggacgat gtactccagg
     1681 gcctgctcgg gcgtcacgtt ccgcagcccc accgtcacct tgccttccac cttgggggca
     1741 atgagaatgt tggcgccgct ctggatggcg agcgccttga gcacatcctg aatatcggca
     1801 ttgacaaagt cgagcgtgac caacctccgc gagggagcct gcgctccgag c
//
LOCUS       DAOSCV010000925         1846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42143_length_1846_cov_3.095477, whole genome shotgun sequence.
ACCESSION   DAOSCV010000925 DAOSCV010000000
VERSION     DAOSCV010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42143_length_1846_cov_3.095477"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..734
                     /locus_tag="PLY56_17735"
     CDS             126..734
                     /locus_tag="PLY56_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOJ23371.1"
                     /translation="MNCTDATAYMGQLADLFARAVSETLTTEVLRGVGVEGMTPSLLA
                     ALECLHRRGVGGVGDLARDLGVTYAAASQFAARLEQKGLLVRQHDARDRRRAALRLST
                     RGAQVVDQVARLRADRLQAILARMPEEQRQALLDALEAFLFHAFEDAAAIERLCGRCR
                     IPHSPQCVVNQMALARTGRGVKRPCCTPRSDAPEGGGTVPNA"
     gene            798..>1846
                     /locus_tag="PLY56_17740"
     CDS             798..>1846
                     /locus_tag="PLY56_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ23372.1"
                     /translation="MSESKVSVDQVRAALGKVMDPELQRDLVSLGMIQNLEVDDGAVS
                     FDLVLTTPACPLRNQIREAAEAAVRAVSGVTEVRVRVTSKVSSRPCADQALLPGVKNV
                     IAVGSGKGGVGKSTVAVNLAVALAEAGARVGILDADIYGPSIPLLMGAEERPLLVDGR
                     LRPLERYGVKLMSLGFLIDGNAPVIWRGPMVAGAVRQLLTDVEWGELDYLVVDLPPGT
                     GDAQLTLAQSVPLTGAVIVMTSQEVALSIASKALLMFRKMDVPVLGVVENMSSFVCPH
                     CEQETPIFGPRGGGEKVAAALDAPFLGSVPLDPAIVEESDQGTPSIAAHPESRQADAF
                     RKIASAMAAQVSQRNL"
BASE COUNT          285 a          536 c          701 g          324 t
ORIGIN      
        1 ggagcttcga gctccgcgcg tgcgggcggc cctctcccta tgtcggaatc tcaggccatg
       61 ctctcaaggt ttgacatgcc tgcctgcatc cagtataatg gggccaggat gtttaagtga
      121 cttacatgaa ttgcactgat gctacggcat atatgggcca gttggcggat ctctttgccc
      181 gggcggtttc ggagactctg acgacggagg ttctccgggg cgtcggcgtg gaggggatga
      241 caccgtcact gctggctgct ttggagtgct tgcatcgccg gggtgtgggc ggagtggggg
      301 atctggcccg cgacctgggg gtgacctacg cggcggcgag ccagttcgca gcccggctgg
      361 agcagaaggg attgctggtc cgccagcatg atgcgcgcga ccgacggcgg gctgcgctcc
      421 ggctgagcac gcggggtgct caggtggtgg atcaggtggc gcgtcttcgc gccgaccgcc
      481 tgcaggcgat cctggcgcgg atgccggagg agcagcgcca ggcgcttctc gatgcgctcg
      541 aggcgtttct tttccatgcc ttcgaagatg ccgcggccat cgagcgcctc tgcgggcgct
      601 gtcgcattcc gcactcgccg cagtgcgtgg tgaatcagat ggctctggcg cggaccgggc
      661 gcggagtgaa acggccgtgt tgtacaccac gatccgatgc ccctgaaggc ggtgggaccg
      721 ttccgaatgc atgagagatc ccggagggcg cgagcgcaac gcgggtgcca tccgggtgct
      781 tactaaagga gaagagagtg agcgagagca aggtgagtgt ggaccaggtg cgcgcagccc
      841 tcgggaaggt gatggacccc gagctgcagc gcgacctggt gtcgctggga atgatccaga
      901 acctggaagt ggacgacggc gccgtctcgt ttgacctggt gctgacgacg ccggcgtgtc
      961 cgctccgcaa ccagatccgc gaggccgccg aggccgcggt gcgcgccgtg tcgggggtca
     1021 cggaggtgcg cgttcgcgtg acgagcaagg tctcctcgcg tccgtgcgcg gatcaggcgc
     1081 tgctaccggg tgtgaagaac gtcatcgcgg tgggcagtgg caaggggggc gtcggcaaat
     1141 ccacggtggc ggtgaacctg gcggtggcgc tggcagaggc aggcgcgcgc gtcggcatcc
     1201 tggacgccga tatctacgga ccgtcgatcc cgctgttgat gggggcggag gagcggcccc
     1261 tgctggtgga tggccgcttg cgcccgctgg agcggtatgg ggtgaagctg atgtcgctcg
     1321 gcttcctcat cgatgggaac gcgccggtga tctggcgcgg gccgatggtg gcgggcgcgg
     1381 tgcgccagct cctcaccgat gtggaatggg gcgagttgga ctacctggtg gtggatctcc
     1441 cgcctggaac cggcgacgcg cagctcacgc tcgcccagtc cgtcccgctc acgggagcgg
     1501 tgatcgtgat gacctcccag gaggtggcgc tcagcatcgc cagcaaggcg ctcctgatgt
     1561 tccggaagat ggatgtgcct gtgctgggcg tcgtggagaa tatgagcagc ttcgtctgcc
     1621 ctcactgcga gcaggagacg ccgatcttcg gcccacgcgg gggcggcgag aaggtggctg
     1681 cggcgctgga tgcacccttc ctgggaagcg tgccgctcga cccggcgatt gtggaggaga
     1741 gcgatcaagg cacgccgagc atcgctgcgc accccgagtc gcgccaggcg gatgccttcc
     1801 gaaagatcgc gagcgcgatg gctgcgcagg tgagccagcg caatct
//
LOCUS       DAOSCV010000926         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42178_length_1845_cov_3.355866, whole genome shotgun sequence.
ACCESSION   DAOSCV010000926 DAOSCV010000000
VERSION     DAOSCV010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42178_length_1845_cov_3.355866"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PLY56_17745"
     CDS             <1..529
                     /locus_tag="PLY56_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOJ23373.1"
                     /translation="DVTVTNAGVGGDYTTRVLARLEKDVLAKKPTHVFVFLGHNDSKL
                     KSTTGYQDAVVPPAVFEQEYREIVQRVRATGARVTVISATSSVYEITKASAEAAAKAG
                     RVHNLFGKPEVMEQFNAIARKVAEENGCGYLDVYEPTRTHPKKASLFTQDGVHINNEG
                     NRLVALEILKYLGKP"
     gene            582..1073
                     /locus_tag="PLY56_17750"
     CDS             582..1073
                     /locus_tag="PLY56_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23374.1"
                     /translation="MTSAEIPIPAGARPAALPLFQLIERVESPGRADWTDIPAGPGNY
                     IVFLPENVPFTISPDAPALEAERIRDKWERINLRKPTDILYIGKAANLRQAIRALARF
                     GAGRGGSARGGEWLWQVAPIRQARLEWMECAPGKEKAMKETLLACFLEEHGEWPLANR
                     RYR"
     gene            complement(1064..>1845)
                     /locus_tag="PLY56_17755"
     CDS             complement(1064..>1845)
                     /locus_tag="PLY56_17755"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOJ23375.1"
                     /translation="HRLLSGDAPQRVPPGPAHACCPLSVRRFIAEWHGYLYSISDEGL
                     WLHHYGAGTLEETLANGMPVRVIQETRYPWEGEIELTLEMPAPADFTFRLRIPEWVEE
                     AYLLLNDRPLDVAAVPGSYARIRRTWLPGDRLCLILPMEPCLIEAHPRVEQLRGQVAV
                     MRGPILYCLESPDLTPGARVSEVHIPADARWSARYRSDLLGGITTLESEAIRVPEGDW
                     RGRLYRPLQRQSERVPIRLVPYYAWANRGVSEMTVWMPVQR"
BASE COUNT          347 a          586 c          635 g          277 t
ORIGIN      
        1 ggatgtgacc gtcaccaatg ccggcgtggg gggtgactat actacgcgcg tgctggcgcg
       61 cctggagaag gatgtgctgg cgaagaagcc gacgcatgtc ttcgtcttcc tcgggcacaa
      121 cgatagcaag ctgaagagca ccaccgggta tcaggacgcc gtggtgcccc cggcggtttt
      181 cgagcaggag taccgcgaga tcgtgcagcg cgtgcgcgcc accggggcgc gggtgacggt
      241 gatctcggcg acctcctccg tctacgagat caccaaggcg agtgcggaag ccgcggcgaa
      301 ggccggacgg gtacacaacc tcttcggcaa gccggaagtg atggagcagt tcaacgcgat
      361 cgcgcgcaag gtggcggagg agaacgggtg tggctacctc gacgtgtacg agccgacgcg
      421 gacccacccg aagaaagcct cgctcttcac gcaggacggc gttcatatca acaacgaggg
      481 gaaccggttg gtggccctag agatcctgaa atacctggga aaaccgtgac acgaagcgag
      541 gcatccagcc acttgcgatg ggaagcaggt tctgagagcc gatgacaagc gccgagatcc
      601 cgatcccagc cggggcgcgg ccggcggcgc ttccactctt ccagctgatc gagcgcgtgg
      661 aatcccccgg tcgtgcagat tggacagaca tccctgcggg gccggggaac tacatcgtct
      721 tcctgccaga gaacgtgccc tttaccatct ctccggacgc ccccgcgctg gaagcggagc
      781 gcatccgcga caaatgggag cggatcaacc tgcgcaagcc gaccgacatc ctctacatcg
      841 gcaaggcggc gaacctgcgc caggcgattc gcgcgctggc gcgttttgga gcgggccggg
      901 gcggctctgc ccgcgggggc gagtggctct ggcaggtcgc gccgatccgc caagcgcggt
      961 tggagtggat ggagtgtgct cctggtaagg agaaggcgat gaaggagacc ctcctcgcct
     1021 gcttcctcga agagcatggt gagtggcccc tcgccaaccg gcgctaccgc tgaaccggca
     1081 tccagaccgt catctcggat actccacggt tggcccaggc gtagtagggg acgaggcgga
     1141 ttggaacgcg ctcggattgc cgctgcaggg gccggtagag ccggcctcgc caatcgccct
     1201 cgggcacgcg gatcgcctcg ctctccaggg tggtgatgcc tcccaggaga tcggagcgat
     1261 agcgggcgga ccagcgcgca tcggcaggga tatgcacctc cgagacgcgc gcgcccggcg
     1321 tgaggtcggg cgactccagg cagtagagga tcggcccgcg catcaccgcc acctgcccgc
     1381 gaagctgctc cacgcgcggg tgggcctcga tcagacacgg ctccatcggc aggatgaggc
     1441 agagccggtc tcctggcaac caggtgcgcc ggatgcgcgc atagctgccg ggaacagcgg
     1501 ccacatccaa cgggcggtcg ttgagcagga ggtaagcctc ttctacccac tcagggatgc
     1561 gcagccggaa ggtgaaatcc gcaggcgctg gcatctccaa cgtcagctcg atctcgcctt
     1621 cccagggata gcgcgtctcc tgaatcaccc gcaccggcat cccattggcc agcgtctcct
     1681 caagcgtgcc ggctccgtag tgatgcagcc acagcccctc gtccgagatg ctgtagaggt
     1741 agccatgcca ctccgcgatg aagcgccgca cgctcaaggg gcagcaggcg tgggctggcc
     1801 caggggggac gcgttggggt gcatctcccg agaggagccg atgct
//
LOCUS       DAOSCV010000927         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42232_length_1843_cov_3.218680, whole genome shotgun sequence.
ACCESSION   DAOSCV010000927 DAOSCV010000000
VERSION     DAOSCV010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42232_length_1843_cov_3.218680"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLY56_17760"
     CDS             <1..615
                     /locus_tag="PLY56_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23376.1"
                     /translation="EEYERWIRWRADHFTEVLRLLRRELRALELERGRRIPVAVRVPS
                     VGLFLNLAQGLDIEQWLREGLVDCLQLDPLETCAGEGVHDVRPYLQLGRRYQVRVIGG
                     VGATWSQGGAAHLAGLRRALGLLEAGVDGIEIYETECLARTSHYRWILPLFGNAPRLK
                     AFLAESNLDACFPVTAATAFYGYDNHSRWDPQGWTVHGSGGAVL"
     gene            complement(631..1341)
                     /locus_tag="PLY56_17765"
     CDS             complement(631..1341)
                     /locus_tag="PLY56_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HOJ23377.1"
                     /translation="MEGGVYVLRATILLLVAWTAVTPLGAAPEERTISINLRNADVRQ
                     VIAYVLRGTDINYVFADDVSGRITCSLFDLPVEQALQAILSAADCESVEEDGALFIRK
                     RTPPPEPAPEKSSPATAASTAGTPNPPPASTQAPENVAPAADKAADAAKEQASPVYII
                     MPVAPSPPPPGPVLYRSPVGSGSVPPVSTPSLSGFLRGPVTPGRAWFEQMAMGSLSQM
                     LAPRPFVSSFIMPGLAPY"
     gene            complement(1411..1827)
                     /locus_tag="PLY56_17770"
     CDS             complement(1411..1827)
                     /locus_tag="PLY56_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015181122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ23378.1"
                     /translation="MVYQEEFILETSGHQEMHDITSQVASIVAKSGIRIGIAHVFQVG
                     STGALGTIEFEPGLRRDLPEMLDRLIPPSRSYGHEQAWHDGNGHSHLQATLLGPDITI
                     PVREGQPVLGTWQQIFHLECDIKPRRRAIVVTVLGE"
BASE COUNT          303 a          583 c          639 g          318 t
ORIGIN      
        1 gaggagtatg aacggtggat ccgctggcgg gctgaccact tcacggaagt gctccgcctg
       61 ttgcgccgtg agctgcgcgc gctggagttg gagcgcgggc gcaggattcc cgtggccgtg
      121 cgcgtccctt ccgtcggcct cttcctcaat ctggcccagg gtctggatat tgagcagtgg
      181 cttcgcgagg gcctggtgga ttgcctccag ctcgacccgc tggagacgtg cgccggcgag
      241 ggcgtgcatg atgtgcgccc ctacctccag ctgggccgcc gctaccaagt tcgagtgatc
      301 ggcggagtgg gggctacgtg gagccaggga ggcgcggcgc atctcgccgg gctccggcgg
      361 gcgcttggct tactggaagc gggagtcgat ggcattgaga tctacgagac ggagtgcctg
      421 gcgcgcacca gccactaccg ttggatccta cctctcttcg gcaatgcccc ccgcctgaag
      481 gcgttcctgg cagagtccaa tctggacgcc tgctttccgg tgacggccgc cacagccttc
      541 tacgggtacg ataaccactc gcgctgggac ccgcaggggt ggacggtaca tggcagcgga
      601 ggggctgtgc tctgagcgcg cccgctcccc tcaatacggg gcgagccccg gcatgatgaa
      661 gctagagaca aacgggcgcg gtgcgagcat ctgggagagc gagcccatcg ccatctgctc
      721 gaaccaggct ctgccgggcg tcactggacc ccggaggaac ccggatagcg agggagtgct
      781 aaccggcggc acgctcccag aacccacggg cgaacggtag aggaccggcc cggggggagg
      841 aggagaggga gcgaccggca tgatgatgta gaccggagag gcctgctcct tggccgcgtc
      901 cgccgcctta tccgctgccg gggcgacatt ctctggcgcc tgggtgctgg cgggcggggg
      961 attcggagtg ccggctgtcg aagcggcggt cgccggcgag ctcttttccg gggcaggttc
     1021 gggaggcggc gtccgcttgc ggatgaagag cgccccgtcc tcctccacgc tttcgcaatc
     1081 agccgcgctc aggatcgcct gaagcgcctg ctcgaccgga agatcgaaca gagagcaggt
     1141 gatcctcccg ctgacgtcgt ccgcgaagac gtagttgata tcggtgccgc gcaagacgta
     1201 ggcgatcacc tggcgcacgt cggcgttcct caggttgatg gagatcgtcc tctcctccgg
     1261 ggccgccccc aacggagtga ccgccgtcca ggcgacgaga agcaagatcg tagcgcgcaa
     1321 cacgtacacg cccccctcca atacccatcc tggcgatgta cgagatccgg gaacctctca
     1381 cccctttgac gagagcgcgg ctgtccaggt tcactctccc aacacggtca ccacgatcgc
     1441 gcgccggcgg ggcttgatgt cgcactcaag gtggaagatc tgctgccacg tgccaaggac
     1501 gggctggccc tccctcacag ggatcgtgat gtctgggccg aggagcgtcg cctggagatg
     1561 ggagtgcccg tttccgtcgt gccacgcctg ctcgtggccg tagctccgac tgggtgggat
     1621 gaggcggtcg agcatctcgg ggaggtcgcg ccggagccct ggctcaaact caatcgtgcc
     1681 gagggcgccg gtgctcccca cctggaagac gtgggcaatt ccgatccgga tgccggactt
     1741 ggcgacgatg gatgccacct ggctggtgat gtcgtgcatc tcctggtggc cagacgtttc
     1801 aaggatgaac tcttcttggt acaccataac accaggccag ttc
//
LOCUS       DAOSCV010000928         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42431_length_1837_cov_2.857464, whole genome shotgun sequence.
ACCESSION   DAOSCV010000928 DAOSCV010000000
VERSION     DAOSCV010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42431_length_1837_cov_2.857464"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="PLY56_17775"
     CDS             <1..1311
                     /locus_tag="PLY56_17775"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23379.1"
                     /translation="FVLATTDPQKVPATILSRCQRMDFRRGTVSDLVSRLEVVVAGEG
                     IKADPDALALIARSADGSWRDALSILEQIIAFGERHITADEVRSILGTVHEELLAECA
                     RAVAAEDATAALRVVAQLADEGKDIRDFLRGLLAHFRNLLLVGASGGAVLPDGATAEE
                     ARLLKEQAAAISPSTCLRAIEALSDAEKEMRRDSQHRLLLEIALLRMVSRYAPASPAA
                     PRESAVPPAPMAPRPTELRQATETPAEPALRRMASPPPSPPSPPSPAPAPEAAPEPPP
                     VAVPSQPSPVQQGQELDLDSVLARWALVMEHVGRGRLRGILSDVRPVGVSARELVLQV
                     RQGYVFHQEQLEKPENIKVIAEAVHRALGVPLAVRVVMEGEAAGRESPPVTAAAPGPA
                     AGGTPASGQPAHAAEPDPFDAETMSVKDVLDLFHGRVIQSKDDN"
     gene            1485..>1837
                     /locus_tag="PLY56_17780"
     CDS             1485..>1837
                     /locus_tag="PLY56_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ23380.1"
                     /translation="MSRKGGFGGLGGLGGGGMQGMIMKQVQKFQEEQERLMAELEEMQ
                     VQGSAGGGVVNATVTGTGDLVAVKIDPDAVDPDDVEMLEDMVVAAVRDALAKAQKGRE
                     EKLGSLMSGLPKIPGL"
BASE COUNT          303 a          596 c          661 g          277 t
ORIGIN      
        1 ttcgttcttg ccacaaccga cccccagaag gtgccagcca cgatcctgtc gcgctgccag
       61 cggatggatt tccgccgcgg caccgtgtcg gacctggtga gccggctgga ggtggtggtc
      121 gccggcgagg gaatcaaggc cgatccggat gcgctggcgc tgatcgcgcg ctcggcggat
      181 ggatcctggc gggatgcact gagcatcctt gaacagatca tcgcgtttgg cgagcggcac
      241 atcaccgccg atgaggtccg cagtatcctc ggcacggtgc atgaggagct tctcgctgaa
      301 tgcgcgcgcg ccgtcgccgc ggaggatgca actgccgcct tgcgcgtggt ggcacagctg
      361 gcggatgagg gcaaagatat ccgtgatttc ttgcgcgggc tgctcgccca ttttcgcaac
      421 ctgctcctcg tcggggcgtc cggcggcgcg gttctgcccg acggagccac cgcggaggag
      481 gcgcgcctct tgaaggagca ggcagccgcc atctcgccaa gcacctgttt gcgcgcgatc
      541 gaggcgctct ccgacgcgga gaaggagatg cggcgcgaca gccagcaccg cctactcctg
      601 gagatcgccc tgctgcgaat ggtctcgagg tacgccccgg cctctcccgc cgcgccgcgg
      661 gaaagcgccg tgccaccggc gcccatggcg cccaggccaa cggaactccg gcaagccacc
      721 gagacgccgg ccgagccagc tctgcgccgg atggcgagtc caccgccgtc accgccatcg
      781 ccaccgtcgc cggcgccggc gcccgaggct gctccagagc caccgccagt ggctgtgccc
      841 agccagccat cgcctgtcca gcaaggtcag gaactcgacc tggactcggt gctcgcccgg
      901 tgggcgctgg tcatggagca cgtgggccgg gggaggttgc gtggaatcct gagcgatgtg
      961 cggcctgtcg gcgtcagcgc ccgcgagttg gtcctacaag tgcgccaggg ctacgtcttc
     1021 catcaggagc agctggagaa gcccgagaac atcaaagtga tcgccgaagc ggtgcatcgc
     1081 gcccttgggg tgccactcgc cgtgcgggtc gtgatggagg gcgaggcggc cgggcgcgaa
     1141 agcccgccgg tgaccgccgc ggcccctggc ccggcggcag gcggcacccc cgcttccggg
     1201 cagccggcgc acgcggccga accggacccg ttcgacgcgg agacgatgag cgtgaaggat
     1261 gtcctggatc tctttcatgg ccgggtgatc cagagcaaag atgacaacta ggggaatggg
     1321 ccggttgagt gggcacgagg aggcgctccg cgcgcccgtt ttgcccgcgc aatcgtgcat
     1381 tccccttcgg ttcaaggttt ggcgagccgc gcgaggctgt tgggccgcga gccaacccag
     1441 ccaccttgaa catccagtag agagcaaccg gaggggagga accgatgtct agaaagggtg
     1501 gtttcggcgg gctgggcggg ctcggtggcg gcggcatgca gggcatgatt atgaagcagg
     1561 tgcagaagtt ccaggaggaa caggagcgcc tgatggccga gctcgaggag atgcaggtcc
     1621 agggtagcgc cggcggcggc gtcgtcaacg ccacggtgac cggcactggc gatctcgtcg
     1681 cggtcaagat cgacccggat gccgtcgacc ccgacgatgt cgagatgctg gaggatatgg
     1741 tggtggccgc cgttcgcgac gcgctcgcca aggcccaaaa ggggcgcgag gagaagctcg
     1801 gctcgctcat gtccggcctc ccgaagatcc ccggcct
//
LOCUS       DAOSCV010000929         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42456_length_1836_cov_3.535093, whole genome shotgun sequence.
ACCESSION   DAOSCV010000929 DAOSCV010000000
VERSION     DAOSCV010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42456_length_1836_cov_3.535093"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            443..1489
                     /gene="cpaB"
                     /locus_tag="PLY56_17785"
     CDS             443..1489
                     /gene="cpaB"
                     /locus_tag="PLY56_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOJ23381.1"
                     /translation="MRRNKYLMTGVLLLILSLILVRAYLHRERHARAREESAVLRDVV
                     VARQTIPKWAMITPEMIELQKVDPTREMVPQDALTSLDAAQGKFTRAEISAGRPVTTG
                     GLLSGDELGLSAHLPPGKRAMTIQVSDTTAVAGFLRPGDRVDVLGTFTGETPVTDTLL
                     QGVQVIAVGKALDQKSLNAVEPNNLVTLAVTPSEARALTFAQTKATIQLVLRPLTESE
                     MALEPSPPPIPQESVRIVARPSQPRARQSTRARARTTRSGVRRSQPPSSTPALSLAPP
                     VLPPAKPELITPIPPPVPSRPTMARSISDGPALPAATPPHTVEVIRGTQRETVTLRVT
                     ESKGIPHEPEQDQR"
BASE COUNT          325 a          634 c          579 g          298 t
ORIGIN      
        1 cacttgattt ttggtggctg atgcgcgtgc aaggtctggc tgaacggggg cacgggcgca
       61 ggcgatgtga agctaatggg gatggtcggg gcgctgcaag gccctcagtt cgcactctgg
      121 acggcgctct ttggcgccgt cgcaggggga atattcgccc tggccgcatt ggcctggaga
      181 ggcggcaccg cccaggtact gcggaacgtg ggtgcgtcga tctaccgcat ggccgcagcg
      241 ggctcgctcg gccgggtgga accgacgtcc caaagctttc gcatgagcta cgcgctcgcc
      301 atcgcactgg gagccgccgc cgcgtactgg tacctgagat agcacaccgc gcccctggcg
      361 cacgacgcgt tgtggcccac gtctccagcc cacccctggc agatcgttcc cgggaagagc
      421 ctgcgcgccg gagagataca gcatgcgtag aaacaaatac ctgatgacgg gagtcctcct
      481 cctgatcctc tccctaatcc tggtgcgcgc ctacctgcac cgcgagcggc acgcgcgcgc
      541 ccgggaagag agcgccgttc tgcgtgacgt ggtggtcgcc cggcagacca tccccaagtg
      601 ggcgatgatc acgccagaga tgatcgagct gcagaaggtg gaccccaccc gtgagatggt
      661 gccccaggac gctctgacat ccctcgacgc cgctcaagga aagttcaccc gggcagagat
      721 cagtgcgggc cgccctgtca ccacgggggg actactctca ggagacgagc tgggtctctc
      781 agcccacctg ccccccggca aacgcgccat gacgatccag gtctccgata cgaccgccgt
      841 ggctggcttc ctccgtccgg gagatcgggt ggatgttctc ggcactttca cgggagagac
      901 gccggttacc gacacgctgc ttcagggggt gcaggtgatc gcggtgggca aggcgctgga
      961 tcaaaaatcg cttaacgcgg tggagcccaa caacctggtc accctcgcgg tcacgccgag
     1021 cgaagcccgg gcgctcacct tcgcgcaaac gaaggcgacc atccagttgg tgctccgccc
     1081 tctcacggag tcggagatgg ccctcgagcc atcaccgcct cccatccccc aagagtccgt
     1141 ccggatcgtc gcgcgcccaa gccagccacg cgcacggcaa tcaacgcgtg ctcgtgcccg
     1201 aacgacgcgc tccggcgtgc gccggtctca gccgccgagt tccaccccgg cactgagcct
     1261 ggcgccccct gtgctgcctc cggccaagcc ggagttgatc actcctatac cgccgccggt
     1321 cccctctcgc cccacgatgg cgagaagtat cagtgatggg ccagcactcc cggcggccac
     1381 tccaccgcat acggtggagg tcattcgggg cacacagcgc gagactgtga cgctccgggt
     1441 aacggaaagc aagggcattc ctcatgaacc cgaacaggac caacgctgat actcttcatg
     1501 gttctcggtc catgtgccct ggggcttctg gccttccgca ccgcctggct gcggcagccc
     1561 tggggttact ggtcgtcttg acgcctaccg gcggtgtggc aagcgcaccc gcgcgcgcat
     1621 caaaagcgcg cgttaccata cagcgcacgc accgggtggc gaagggcgaa acggcagtgc
     1681 gcatcgccaa gcgttacggc atctcgcttc aggatctcct gcaagccaac aagatcaccg
     1741 atcccgatag agtcctggcg ggctctctcc tggtgatccc gaaggccggt tacgccgcgc
     1801 gcgtcgccaa ggcgaagccc gcgtctccca aggctg
//
LOCUS       DAOSCV010000930         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42693_length_1829_cov_3.362458, whole genome shotgun sequence.
ACCESSION   DAOSCV010000930 DAOSCV010000000
VERSION     DAOSCV010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42693_length_1829_cov_3.362458"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLY56_17790"
     CDS             complement(<1..757)
                     /locus_tag="PLY56_17790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ23382.1"
                     /translation="MLTDPASPSRLDYKWKALIAVAIGTFMSTLDSSIVNLAMPTLGH
                     EFGVEITTVEWVAMAYMLALAGLLISMGRLSDMVGHKRVYNVGFAIFTLGSLLCALAQ
                     GIHALILFRVVQGAGAAMLASNGAAILTAAFPRSERGKAMGLNGTIVGAGLTVGPSLG
                     GLLIHAFGWRSIFTINLPIGVIGILLASRLLRPDSPVANNERFDLPGALALLSSLTAL
                     LLALSRGEAWGWSSTPTVGLFALSLASLILFIRI"
     gene            981..1733
                     /locus_tag="PLY56_17795"
     CDS             981..1733
                     /locus_tag="PLY56_17795"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOJ23383.1"
                     /translation="MAQPRIGIQLIIYGKRWQEDLAGVLREVAGAGYDGIECGDLAAY
                     YPVEQISQLLAETGLQLVGVHAGFNNVADPSKLNASLDLVTALGGSYLVCSGVGQIQG
                     IETYDQAAGVFNKAGEVCRERGVTFCYHNHAWEFEELGGEKGIHRLIARTDPALVQLC
                     VDLYWVHIGGENPVSFVERYAERVGYYHVKDGAKGSFTELGRGTVDLPNALRAALRTN
                     PDWLVYEQDRTDKEPAQSIMESRQYLRDTIGL"
BASE COUNT          364 a          551 c          634 g          280 t
ORIGIN      
        1 cgatgcggat aaagaggatc aggctcgcga gggagagcgc gaacaacccc acggtggggg
       61 tggatgacca gccccatgct tctccccgac tgagcgccag gagaagcgcg gtgaggctgg
      121 agagaagtgc gagcgcaccg gggagatcaa agcgctcgtt gttggccaca ggcgaatcgg
      181 ggcgaagcag gcggcttgcg aggaggatac cgatgacgcc tatcgggagg ttgatggtga
      241 agatggagcg ccacccaaac gcatggatta gcaggccgcc gagcgaaggg ccgacggtga
      301 gccctgcgcc cacgatcgtg ccgttcaggc ccatcgcctt gccccgctcc gaccgcggaa
      361 acgcggcggt gagtatggcg gcgccgttcg acgcgagcat cgccgcgccg gcaccttgca
      421 ccacccggaa caggatcagc gcgtggatcc cttgggccag ggcgcacagc agtgacccga
      481 gggtgaagat ggcgaacccc acgttgtaga cgcgcttatg ccccaccatg tcggaaagcc
      541 ggcccatgct gatgagcagc ccggcgagcg ccagcatgta ggccatagcg acccattcga
      601 cggtcgtgat ctccaccccg aactcatggc cgagcgtggg catcgccagg ttcacgatgc
      661 tggaatcgag ggtggacatg aaggtgccga tcgccacggc gatcagggct ttccacttgt
      721 aatcgagacg cgacggcgag gcgggatcgg tcaacatcag gttctttctc tccgacggca
      781 ttctaccacg gcgggagaga tcggcgcaag gttgtgaagg gccccacgcc gaacgtggag
      841 agcagcacca ggcatgacct agccgcccgc tcgcatgggc aggggtggca acacgcgggc
      901 gtgaacaccc gcgccgcgac aagcagcccg cgctccatgc cgggtgcgac atccgtgaag
      961 agattggagc ggaggaagag atggcacagc ccagaatcgg gatccagttg atcatctatg
     1021 ggaagcggtg gcaggaggac ctggcgggag ttctgcgcga ggtcgccggc gccggctacg
     1081 acgggatcga gtgcggcgat ctggccgcct actacccggt cgagcagatc agccagctcc
     1141 tcgctgaaac cggcctgcag ctcgtgggcg ttcatgccgg tttcaacaac gtggcagacc
     1201 cctcgaagct gaacgcgagc ctcgatctgg tgaccgcgtt gggcggcagc tacctcgtct
     1261 gctccggcgt tgggcagatt caggggatcg agacctacga ccaggcagcc ggcgtcttca
     1321 acaaggccgg cgaggtatgc cgcgagcgcg gggtgacctt ctgctatcac aaccacgcct
     1381 gggagtttga ggagctcggc ggtgagaagg ggatccatcg cttgatcgcg cgcactgatc
     1441 ccgcgctggt gcagctctgc gtggatctct actgggtcca catcggcggc gagaaccccg
     1501 tctcgttcgt cgagcgctac gctgagagag tcggctatta ccacgtcaag gatggggcta
     1561 agggcagctt caccgaactg ggccgaggaa cggttgacct gccgaatgcc ctccgggccg
     1621 cgctgcgcac gaatccagac tggctggtgt atgagcagga ccgcacggac aaagagccag
     1681 cgcaatcgat catggagagc cggcagtacc tgcgcgatac aatcggtctc tgatcgcttc
     1741 ggaatctggg gcgggggcat ggagccaggg aggcagcaag ccgcgcgcct cctgaagccc
     1801 gttacattgc gcccgccctg gagtgctta
//
LOCUS       DAOSCV010000931         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_42798_length_1826_cov_3.158667, whole genome shotgun sequence.
ACCESSION   DAOSCV010000931 DAOSCV010000000
VERSION     DAOSCV010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42798_length_1826_cov_3.158667"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PLY56_17800"
     CDS             <1..673
                     /locus_tag="PLY56_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010283220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ23384.1"
                     /translation="REHLARLSERTEALDRQRLRRLHYRAPGTDLVIELPERHQWLSC
                     AGSRNAQGVCFVPNMPTEEVFTVPARNGVHGTVRSTMPLNYNGVLIQDLSLRFENGRI
                     VDFSASTGLDALKGLIETDEGSHYLGEVALVPVDSPISQLGVLFYNTLLDENASCHLA
                     IGRGFPVCIEGGASMSKEELAHHGVNDSIAHVDFMVGSRDLEIDGETADGTVPIFRNG
                     TWATP"
     gene            complement(755..1483)
                     /locus_tag="PLY56_17805"
     CDS             complement(755..1483)
                     /locus_tag="PLY56_17805"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOJ23385.1"
                     /translation="MSESQLAGFDRHAPCTAARDGLASGRQRRDAMRIGIIGAGMVGG
                     ALGKRWASLGHQVLFGVRDPAAPKVKQLLADAGPNACAGSVAEAAAFGPVVVLAVPWS
                     AAREAIQAAGDLTGKILIDPTSPIKRDLSGLDVPEGSSAAEQIAAWAPGARVVKAFNT
                     VSHTAMTDPRFPEGNASVFYAGDDDEAKRVVAELISEIGLTPEECGPLANARLLEVVG
                     LLRMYLAAYAPASVTGVAFRVLRR"
     gene            1669..>1826
                     /locus_tag="PLY56_17810"
     CDS             1669..>1826
                     /locus_tag="PLY56_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOJ23386.1"
                     /translation="MLARVESAAIHGIDAYIVQVEVDIVPAQLPGFTIVGLPDTAVQE
                     SRERVRAA"
BASE COUNT          320 a          647 c          573 g          286 t
ORIGIN      
        1 gcgcgagcat ctcgcccggc tctcggagcg aacggaggcg ctcgaccgac agcgcctgcg
       61 ccggctgcac taccgggcgc caggcaccga cctggtgatc gagcttcccg agcgccacca
      121 gtggctgagc tgcgccggct cgcgcaacgc gcaaggcgtc tgcttcgtgc ccaacatgcc
      181 cacggaggag gtcttcacgg tgccggcccg caacggcgtc catggcaccg tgcggagcac
      241 catgcccctc aactacaacg gcgtcctgat tcaggacctc tctctccggt tcgagaatgg
      301 ccggatcgtc gacttctccg ccagcaccgg cctggacgcg ctgaagggcc tcatcgagac
      361 agacgagggc tctcattacc tcggggaggt ggcgctcgtg ccggtcgact ccccaatctc
      421 gcaactcggg gtcctgttct acaacacgct ccttgacgaa aacgcttcat gccacctggc
      481 gatcgggcgg ggcttccccg tctgcatcga gggaggcgcg tcgatgagca aggaggagct
      541 cgcccatcac ggggtcaacg acagcattgc gcacgtcgat ttcatggtgg gctcgcgcga
      601 tctggagatc gatggcgaga cggcagacgg caccgtgccg atcttccgaa acggcacctg
      661 ggccacgcca taacgcggag ccagccagcg gatttcaccg gcatgcgtga cggatcggga
      721 ccacgacgct cccgatccgt cacgcgctct ccccctagcg gcggaggacc cggaacgcta
      781 cgccagtaac gctcgcggga gcataggccg ccaggtacat gcggaggagg cccaccactt
      841 cgagcagccg cgcgttcgcc aggggcccgc actcttccgg cgtgaggccg atctcggaga
      901 tcagctccgc gacaacacgc ttcgcctcgt catcatcgcc ggcgtagaag accgaggcat
      961 tcccctcggg aaagcgcggg tcggtcatcg ccgtgtgcga aacagtgttg aatgccttca
     1021 ccacgcgcgc cccgggggcc caggcggcga tttgctctgc ggccgaagag ccttccggca
     1081 catccaggcc gctgagatcg cgctttatcg ggctggtcgg atcgatcagg attttccctg
     1141 tcaaatctcc cgccgcttga atggcctcgc gggcggcgct ccatggcacc gcgaggacga
     1201 cgaccgggcc gaacgccgcc gcctccgcga cgctgccggc acaggcgttg ggtccggcgt
     1261 cggcaaggag ctgcttcacc ttgggcgcgg ctggatcgcg cacgccaaag agcacctggt
     1321 gccccaggct ggcccatcgc ttgcccagtg ccccacccac catccctgcg ccgatgatcc
     1381 caatccgcat cgcatccctc cgctggcggc cagatgccag gccgtcacga gcagcggtgc
     1441 atggagcgtg cctgtcaaac cccgccaact gagattccga cacaaggaga aggccgcgcg
     1501 ccgggttgca ccctgcctcc gaggcttcgg attccttata tcggaatctc agaccggcat
     1561 gcacgcccca ctccaccgcg tctgaaaaca cgcagttctc tgacagaagg aattggtgcc
     1621 gggcgcgcga agatcggccc aattagcgcg ctccaggagg acacggccat gctcgctcgt
     1681 gtggaaagtg ccgccatcca tgggatcgac gcctacatcg tgcaggtgga agtggatatc
     1741 gtcccggcgc agctccccgg cttcaccatc gttggcctgc cggacaccgc ggtccaggag
     1801 agccgcgagc gcgtccgcgc cgccat
//
LOCUS       DAOSCV010000932         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43003_length_1820_cov_2.439093, whole genome shotgun sequence.
ACCESSION   DAOSCV010000932 DAOSCV010000000
VERSION     DAOSCV010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43003_length_1820_cov_2.439093"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="PLY56_17815"
     CDS             <1..674
                     /locus_tag="PLY56_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ23387.1"
                     /translation="TPFYQDLFARHGVRRLLDVGAGTGWHARLFAQWGIEVVALDPSA
                     AMLEKAREVTAGLPVQLVQAGFEDLPQAVNPPFEAVVCLGNTLPHAATHAARMQALRG
                     FRACLGSGGVCVVQTLNYDQLAATHERFQPLSHGVVNGQEQLLLRMFDFGPETWRFNI
                     LRFTRVDSEWRFDVSSATHLPVFGAELERQMREAGFAEVRLLGGFDGSVFNPETSDML
                     LAVAS"
     gene            complement(744..>1820)
                     /locus_tag="PLY56_17820"
     CDS             complement(744..>1820)
                     /locus_tag="PLY56_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23388.1"
                     /translation="TVALLAEVQVRTGNESFTVADQVTLPCDTPFLAALAERKRTLVA
                     GRPAEVAVEIRNVMPHEIQVRSRPVLPKGWSWQLAGPETVTVGPAGLEPGTATVRLTI
                     TAPPDARPGTVRIPIETSYTNASGSSIFCELACDLLPRLAPVAGKGAGFEPSPGPARV
                     RQRGRALLYAEAGETISLKVTNVRVTTFRDTASYRLLDPDLKELAKGAVKVDESATVT
                     VKAEKTGTHFVEIVPKGGSCTIESENRCLVLEASESEPLQVIYQMPVSYLYVPENARE
                     FTLRVKCGGETEPVELVLTDPSGREALRQSGALLENEFKISVPPEGRGKAWKLEMTPK
                     EDVLFFLAGDVVPFLADHPARLLK"
BASE COUNT          279 a          644 c          599 g          298 t
ORIGIN      
        1 agacgccttt ctaccaggat ctcttcgcgc ggcatggagt ccgccggctg ctggacgtgg
       61 gcgcgggcac gggctggcac gcccggctct tcgcccaatg gggaatcgag gtggtcgccc
      121 tggacccaag cgccgcgatg ctggagaagg cacgcgaggt gacggcgggc ctgccggtgc
      181 agctcgtgca ggccggtttc gaagacctgc cgcaagcggt aaacccgccg ttcgaggcgg
      241 tcgtctgcct ggggaacacc cttccccacg cggccacgca cgccgcgcgc atgcaggcgc
      301 ttcgcggctt ccgcgcgtgc ctgggatcgg gcggagtatg cgtggtgcaa accctcaact
      361 acgatcaatt ggccgccaca cacgagcggt tccagccgct ctcgcacggc gtcgtgaacg
      421 gtcaggagca gctcctcctg cggatgttcg acttcggacc ggagacgtgg cggttcaaca
      481 tcctgcggtt cacgcgcgtc gacagcgagt ggcggttcga cgtctccagc gcaacgcacc
      541 ttcccgtttt cggcgcggag ctcgagcgcc agatgcgcga ggccggcttc gcggaagtgc
      601 gcctgctcgg cgggtttgac ggctcggtgt tcaacccgga aacgtcggat atgctcctgg
      661 ccgtcgccag ctgatccggc agcgctggct caaaagtggc cgggcgcgcg gtctcgggat
      721 gagtaccgcg cgcccggcac cggtcacttc agcagccggg cggggtggtc ggccaggaac
      781 ggaaccacgt cgcccgccag gaagaacaaa acgtcctcct tcggcgtcat ctccagcttc
      841 cacgccttcc cccggccctc cggcggcaca ctgatcttga actcgttttc gaggagcgcg
      901 ccactctgcc tcaacgcctc gcgcccggag ggatcggtga gcaccagctc caccggctcc
      961 gtctcgccgc cgcacttcac gcgcagggta aactcgcgcg cattctctgg cacgtagagg
     1021 taggaaaccg gcatctggta aatcacctgg agtggctccg actccgacgc ctccagcacc
     1081 aggcaacggt tctcgctttc gatggtgcat gagccgccct tcggcacgat ctcgacgaag
     1141 tgcgtgccgg tcttctcggc cttcaccgtg accgtcgcgc tctcatcgac cttgaccgca
     1201 cccttcgcca gctccttcag gtcgggatcg agcagccggt agcttgcggt atcgcggaag
     1261 gtagtcacgc ggacgttcgt caccttcagc gagatcgtct cgcccgcctc ggcatagagg
     1321 agcgcacgcc cgcgctgccg gacccgcgcc ggtccgggag acggctcgaa cccggccccc
     1381 tttcccgcca cgggagcgag cctggggagg aggtcgcacg ccagctcaca gaagatggac
     1441 gagccggacg cattggtata gctcgtttcg atcgggatgc gcacggtgcc cgggcgcgca
     1501 tccggcggcg ccgtgatcgt gaggcgcacg gtcgccgtcc cgggctccag acccgccggg
     1561 ccaaccgtca ccgtctccgg tccggccagc tgccacgacc accccttggg gagcaccggg
     1621 cggctgcgaa cctggatctc gtggggcatc acgttgcgga tttctacggc gacttcagcg
     1681 ggccgccccg ccaccagagt tcgcttgcgc tcggccagcg ccgccaggaa cggcgtgtcg
     1741 cacggcagcg tcacctggtc agccacggtg aagctctcgt tgcccgtgcg cacctgcacc
     1801 tcggccagga gagcgaccgt
//
LOCUS       DAOSCV010000933         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43017_length_1819_cov_3.105442, whole genome shotgun sequence.
ACCESSION   DAOSCV010000933 DAOSCV010000000
VERSION     DAOSCV010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43017_length_1819_cov_3.105442"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(307..900)
                     /gene="hisB"
                     /locus_tag="PLY56_17825"
     CDS             complement(307..900)
                     /gene="hisB"
                     /locus_tag="PLY56_17825"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOJ23389.1"
                     /translation="MATTPRRATIERRTTETEIHLELDLDGTGQYDIDTGVGFLDHML
                     AQIARHGRIDLKIRTKGDTHIDFHHTAEDTGIVLGEAIRQALGNKAGIVRYGSAIVPL
                     DEALVQCALDLSGRPFLACHLPVTGKVGEFDAELAEEVFRAIAFNGGITLHLTALAGK
                     NTHHLIEAAFKAFARALRVAKEVEPGSNEIPSTKGVL"
     gene            1139..>1819
                     /locus_tag="PLY56_17830"
     CDS             1139..>1819
                     /locus_tag="PLY56_17830"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HOJ23390.1"
                     /translation="MRNRMRVVFLALLWAAGLSVAGADPENLLRDPGFEQVSGGAFAW
                     WNTPTYWQGKLSMADEAHGGRRAALLATTERAGKWLGRVYNPVTLAITQGARYRVSIW
                     ARGKGDCVVGCIEYSRKEGKSTYQYVPLDPPVPLSDAWQPITFYYTPRDPLATTVAFY
                     AEVRGEGSQVLLDDAFIGLEPLPGYALTVRPEHTMVPVGATLRFRIEASGPAGRQPDA
                     VIACVGGGS"
BASE COUNT          285 a          591 c          619 g          324 t
ORIGIN      
        1 tgccgccccg cgccggtgtc tctccaagcg cggacctatg cgaagtcgcc ggatcggaga
       61 tcgccgggcg ctaccccaca ctggttgcca gcgattcttc tcccgttcct tagaactcta
      121 cctggcgatg ccgcccccgc ccggagcatc cggcagcgcc ggatagctcc cgggggcgca
      181 cggccggcca cctccccaac agacgcccac gaccaacctt gcggcagaac cccccgttgc
      241 ctcggaacga gccacaggta ggagtcatcg gctcccgggt gggtccggga tgcccataca
      301 catgcgctat agcacgccct ttgtcgaggg gatctcgttc gagcccggtt ccacttcctt
      361 ggcgacgcgc agcgcgcgcg cgaacgcttt gaacgcggcc tcgatcaggt ggtgcgtatt
      421 cttgcccgcc agcgccgtga ggtgcagcgt gatcccgccg ttgaaggcaa tggctcggaa
      481 cacctcctcc gccagctcgg cgtcgaactc gcccaccttc ccggtgacgg ggagatggca
      541 tgccaggaag gggcggccgc tcaggtcgag ggcacactgc acgagggctt cgtcgagggg
      601 gacgatcgcg ctgccatagc gcacgattcc cgccttattt cccaacgcct gccggatcgc
      661 ttcgcccagc acgatccctg tatcctcggc ggtgtggtgg aaatcgatgt gcgtgtcgcc
      721 cttcgtccgg atcttcaggt cgatccggcc atgtcgggca atctgggcca gcatgtgatc
      781 gaggaagccg acgcccgtgt cgatatcgta ctggccggtg ccatccagat ccagctccag
      841 atggatctct gtttcggtgg tgcgccgttc gatggtggcc cggcgcgggg tagttgccac
      901 gctcttctcc tcccaaagaa ttgtcgtagg aaacattcca tcgcgggcgg gcgtttccct
      961 gctcgtgccg ggcacgcttg ccgcggggca ggccgaggcg cgagtgcccc tctggtagga
     1021 aaagggcggc gccatgtcgc attaggaggg ggcgctgcca acaacctgcc cgcagggaag
     1081 gggagcatcg gggccggagg tacggcgtct gcttcctggc gcgccgtgga gaacgaagat
     1141 gcgcaatcgg atgagagtgg tgtttctggc tctcctgtgg gccgccggtc tctctgtggc
     1201 gggggcagat ccggagaacc tcctgagaga cccgggcttt gaacaggtga gtgggggagc
     1261 ctttgcctgg tggaatacgc ctacctactg gcaagggaag ctgagcatgg ccgatgaggc
     1321 gcatgggggc cggagggccg ccctgctcgc cacgaccgag agggccggca agtggctggg
     1381 ccgggtttac aacccggtga cgctcgccat cacccagggg gcgcgctacc gggtttcgat
     1441 ctgggccagg gggaagggcg attgcgtcgt cggctgcatc gagtatagca ggaaagaagg
     1501 gaaatccacc taccaatacg ttcccctgga cccgcccgtg ccgctctcgg acgcgtggca
     1561 gccgatcacc ttctattaca cgccccggga tcctctggcg acgacggtgg ccttctatgc
     1621 cgaggtgcgt ggcgagggca gtcaggtgct cctggatgat gcctttattg gcctagagcc
     1681 gcttcccggc tacgcgctca ccgtgcgccc ggaacacact atggtcccgg tgggcgccac
     1741 gctccgcttc cggatcgagg cctcggggcc tgcgggccgg cagccggatg cggtgatcgc
     1801 ctgcgtgggc ggcggcagc
//
LOCUS       DAOSCV010000934         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43052_length_1818_cov_3.250142, whole genome shotgun sequence.
ACCESSION   DAOSCV010000934 DAOSCV010000000
VERSION     DAOSCV010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43052_length_1818_cov_3.250142"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLY56_17835"
     CDS             <1..433
                     /locus_tag="PLY56_17835"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ23391.1"
                     /translation="GVLLILLGVGLMIADIKVPSHGILTAAGAVAFGFGSFMLVRSPD
                     PFLRVSLRLILATTVFTTLFFAFIVGAGLRAQRLRVATGIEGLLGEPVLAKTDLAPDG
                     QVLAEGELWSAENVGEEPIREGDQAVVVAVDGFVLKVRKET"
     gene            435..1298
                     /locus_tag="PLY56_17840"
     CDS             435..1298
                     /locus_tag="PLY56_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ23392.1"
                     /translation="MGGLIEALGPFAFFLLIAAVFVITSAVKVVREYERGVIFRLGRL
                     VGAKGPGLFFIIPGIDTMTKMDLRVITMNVPKQEGMTRDNVPVTVDAVVYFRVVNPSD
                     AVVKVENYAAATSMYAQTTLRAVIGQVEMDQLPSERDEINRRIQGILDQHTEPWGVKV
                     TATEIRDVVIPDGMKRAMARQAETERERRSKIIAAEGEYQAAEKIAQAAAVIGREPVG
                     LQLRYLQSLSEIATEHNSTTIFPIPIDLFKPFLEAAQSRAGAPVPQAASTETAASAPE
                     KPAPPQVEPGA"
     gene            complement(1394..>1818)
                     /locus_tag="PLY56_17845"
     CDS             complement(1394..>1818)
                     /locus_tag="PLY56_17845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23393.1"
                     /translation="GTCWNLVAYPYIKNTGVFRCPSDAAPLLIQPGANPCGGSYKGVP
                     ASAVAHQLSYGFNLQQQNKALTQIQDVSSVMMLVEARYPWGEVGPASKYSYANSSTMR
                     NRHSDGSNIAFMDGHVKWFKSDAILAGKGMKNIDSDAG"
BASE COUNT          301 a          569 c          598 g          350 t
ORIGIN      
        1 cggcgtgctt ctcatcctcc tgggtgtcgg tttgatgatt gcggatatca aggtgccgag
       61 ccatggcatc ctcacggccg cgggagcggt cgccttcggc tttggctcct tcatgctcgt
      121 gcgatcccca gaccccttcc tccgggtctc tctccgtctc attctggcga ccaccgtttt
      181 caccacgctc ttcttcgcgt ttatcgtggg tgccggcctc cgcgcccagc gccttcgggt
      241 ggcgacgggc atcgagggcc tgctgggtga gccggtgctt gcgaagacgg acctcgcgcc
      301 agacgggcag gttctggcgg aaggcgaact gtggagcgcc gaaaacgtgg gggaagagcc
      361 aatccgagag ggcgaccagg cggtcgtggt cgctgtggat ggcttcgtcc tgaaagtcag
      421 aaaggagacg tgatatgggc ggtctgatcg aggctctcgg tccctttgcg tttttcctcc
      481 tcatcgccgc ggtgtttgtc atcacttctg cggtgaaggt ggtgcgcgag tatgagcgcg
      541 gtgtcatctt tcgcctgggc cggttagttg gcgctaaggg tcccgggctt ttcttcatca
      601 tcccgggcat cgacaccatg acgaagatgg acctgcgcgt gatcaccatg aacgtgccca
      661 agcaggaggg catgacgcgc gacaacgtgc ccgtgacggt ggatgccgtg gtctacttcc
      721 gcgtggtaaa cccgagcgac gccgtggtga aggtggagaa ctacgccgct gccacctcga
      781 tgtacgcgca gacgaccctg cgcgctgtca tcggccaggt ggagatggac cagctcccct
      841 cagagcgaga tgagatcaat cggcggatcc aggggattct cgaccagcac accgagccct
      901 ggggcgtgaa ggtcaccgca accgagattc gcgacgtggt gattcccgat gggatgaagc
      961 gcgccatggc ccgccaggcg gagaccgaac gcgagcgacg ctccaagatc atcgctgctg
     1021 agggcgagta tcaggctgcg gagaagatcg cccaggccgc tgcggtgatc ggtcgcgagc
     1081 ctgtgggcct gcagctccgc tatctccaat cgctgtccga gatcgcgacg gagcacaact
     1141 cgacgacgat tttccccatc ccgatcgatc tcttcaagcc gttcctggag gcggcgcagt
     1201 cgcgcgcggg cgcgcccgtg ccgcaggcag caagcacgga gaccgccgcc agcgcgcccg
     1261 agaagccggc cccgccgcag gtcgagcctg gcgcgtagcc gcgctctcgg cccgcgcaac
     1321 aacaaagatg ggcgggaacc ggggtttccc ccgcgttccc gcccattcgc gttatcagga
     1381 gccgccgcca ggtctagccg gcgtcggagt cgatgttctt catgcccttg cctgccagga
     1441 tggcgtcgct cttgaaccac ttcacgtggc catccatgaa ggcgatgttg gagccgtcgc
     1501 tgtggcggtt cctcatcgta gacgaattcg cgtagctgta cttcgaggca gggccaacct
     1561 ctccccacgg gtacctcgcc tcgaccagca tcatcaccga ggagacatcc tggatctgag
     1621 ttagcgcctt gttctgctgc tgcaggttga aaccgtagct cagctgatgc gcgaccgcgc
     1681 tcgccggaac tcctttgtag ctgccgccgc aggggttcgc gccgggttgg atgaggagcg
     1741 gcgccgcgtc ggaagggcag cggaagacac ccgtgttttt gatgtagggg taggccacca
     1801 ggttccagca tgtcccgg
//
LOCUS       DAOSCV010000935         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43208_length_1814_cov_2.831723, whole genome shotgun sequence.
ACCESSION   DAOSCV010000935 DAOSCV010000000
VERSION     DAOSCV010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43208_length_1814_cov_2.831723"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="PLY56_17850"
     CDS             <1..1768
                     /locus_tag="PLY56_17850"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ23394.1"
                     /translation="LAQAQSLTRADPVAIWLVDRAHSELVLSATRARIPERRVRADAT
                     RIPLDSMHLLAESIRQRVVMTAEADTTVQEEGLEACLIGVSRMIVIPLVTHGTPMGAL
                     VAGFPMRFDTDALQVLREIASDAAMAIENAQLYSEARRHVIELEAMRAQAERKEQQAR
                     FLAEAGQLFTMTLDVRATMEAVVTKAAELLGDTCLILERLPGEETLSVAACYEDEAIV
                     AHSLRQHLTAHPVSVGSGLLGRSASTGQACIAHGRPAAPGELGEAIVALGVRGMIAVP
                     LWGRGRVRGVLVAAYYGEERLPTEEDLQLAVLLADRAAVAIENADLFEETQRAGVALE
                     AWGHELERRVEMKTRELRDAQRGLLRAERLASIGQLAATVAHELRNPLNVIKVAHFAL
                     EQGCSPEREKRHLDAIARQVAASSRIINGLLDFARDSPPQYSLVDLGALLHQVLADAD
                     LPQEIHVSTEIPASLPPLLADREQIAQVLSNIIDNAVQVMPQGGELAIHVWWEEWAAY
                     ISVRDTGPGIPEEVRSRIFEPLFTTRTKGTGLGLAIARRIIEAHGGEITASTSPAGGA
                     TFTIMLPLPESAHSRESASSSA"
BASE COUNT          311 a          620 c          581 g          302 t
ORIGIN      
        1 gctggcccag gcgcaatccc tgacgcgcgc ggacccggtg gcgatctggc tggtcgaccg
       61 tgcccattca gagctggtgc tctctgctac gagagcccgc ataccagagc ggcgcgtgcg
      121 cgccgatgcc acacgcattc ccctggactc gatgcacctg ctcgccgaga gcatccggca
      181 gcgcgtggtg atgaccgcgg aagcggatac gactgtgcag gaggagggtc tggaagcctg
      241 tctcattggg gtcagccgga tgatcgtcat tccactcgtg acgcacggta cgcccatggg
      301 cgcgctcgtg gccggtttcc ccatgcggtt cgataccgac gcactgcagg ttctccggga
      361 gatagccagc gacgcggcca tggccatcga gaatgcccaa ctctactctg aagcgcgccg
      421 acatgtcatc gagctcgagg cgatgcgcgc ccaggcggag cgaaaggagc aacaggcccg
      481 gttcctggcc gaagccggac agctctttac catgacgctc gatgtccgcg ccaccatgga
      541 ggctgtcgtg accaaggctg cggaactcct gggcgacacc tgtctcattc tggagaggct
      601 tcccggggag gagacgctga gcgtggcggc ctgttacgag gacgaagcca ttgtcgcgca
      661 tagcctccgc cagcacctca ccgcccaccc tgtgagcgtg gggagcggcc tgctcgggcg
      721 atccgcatcc actggccagg cgtgcatcgc acatggccgg cccgctgcgc cgggcgagtt
      781 gggagaggcg atcgttgcct tgggggtccg tggcatgatc gccgtgccgc tgtggggccg
      841 gggaagggtg cgcggcgttt tggtggccgc ctactatggc gaagagcgct tgcctaccga
      901 ggaagacctc cagctggccg tgctactggc ggaccgcgcc gcagtggcca tcgagaatgc
      961 ggatctcttc gaggagaccc agagggccgg cgttgcccta gaagcctggg gccacgagct
     1021 ggagcgccgc gtggagatga agacccgcga gctccgcgac gcccaacgcg gtctccttcg
     1081 cgccgagcgc cttgcctcca tcggccaact cgcggcgacg gtcgctcacg agctacgcaa
     1141 ccccctcaac gtcatcaaag tcgcgcattt cgccctggag cagggctgct cgcccgaacg
     1201 ggagaaacgc catctggatg ccatcgcacg ccaggtggcc gcatccagcc ggattatcaa
     1261 cggcttgctc gatttcgctc gcgactcgcc cccccaatat agcctggtcg atctcggggc
     1321 gctcctccac caggttctcg cagatgccga cctgccgcaa gagatccacg tgagcacgga
     1381 gatccccgcg tcgcttcccc ctctcctggc cgaccgcgag cagatcgccc aggtgctctc
     1441 caatatcatc gacaacgccg tgcaggtcat gcctcaaggg ggagagctgg ccatccatgt
     1501 ctggtgggaa gaatgggcgg cgtacatctc cgtgcgcgac accggccccg gcatccctga
     1561 ggaggtgcgt tcacgcatct tcgagccgct ttttaccacc cgcacgaaag gcaccggtct
     1621 tggattggcc atcgcacgcc ggatcatcga ggcgcacggc ggcgagatca ccgcatccac
     1681 atcccccgcc ggcggggcca ccttcacgat catgcttcct ctccctgaaa gcgcgcattc
     1741 gcgagaaagc gcgtcttcct ctgcgtaggc gcgggacacg agttcgcccg aaccagcctc
     1801 gcactcggcc gatt
//
LOCUS       DAOSCV010000936         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43228_length_1813_cov_3.554608, whole genome shotgun sequence.
ACCESSION   DAOSCV010000936 DAOSCV010000000
VERSION     DAOSCV010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43228_length_1813_cov_3.554608"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PLY56_17855"
     CDS             complement(<1..148)
                     /locus_tag="PLY56_17855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ23395.1"
                     /translation="MNARKARGFTLIELLVVIAIIAILAAILFPVFARARENARKSNC
                     QSNLK"
     gene            423..>1813
                     /locus_tag="PLY56_17860"
     CDS             423..>1813
                     /locus_tag="PLY56_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ23396.1"
                     /translation="MAVQREMDAPDWQNVQVLSINREPAHASLLPCPDEECALSGERG
                     ASPWFRLLNGNWRFMLVPYPDATPEGFEAEEFDASEWDTIPVPSNWQMHGYDRPQYTN
                     INYPYPMDPPFVPDDNPVGLYRRAFVVPEAWLGRRIYLTFEGVNSAFYLWVNGHRVGY
                     SQGSHMPAEFDITPYTRLGGNVVAVQVFKWSDASYLEDQDFWRLSGIFRDVYLTARSD
                     VYLRDVFVRTPLDAEYRDATLEVDVSVRNAGDAPVAGYVVSAKLIDPDGEGALPETTV
                     GAVDLAAGEEKTLALKAPVSDPDKWTAETPYLYSLLLTLKDAKGEVLEVEHVRVGFRQ
                     VEIRDGQFLVNGVSIKLQGVNRHDTHPDLGHAVSLDSMVQDIMLMKQHNINCVRTSHY
                     PNDPRWYDLCDEYGMYVIDEADLETHGFGYEAPDIPAKRPEWREAFLDRAVRMVERDK
                     NHPCIVMWSLGNES"
BASE COUNT          345 a          549 c          574 g          345 t
ORIGIN      
        1 gcttcagatt gctctgacag ttggacttcc gtgcgttctc tcgcgcccgg gcgaaaaccg
       61 ggaagagaat cgctgccagg atggcgataa tcgctatcac gaccagcagc tcgatcaggg
      121 taaacccgcg tgcttttcga gcgttcatgt ccgtgctcct tccgaaacct caagagcggt
      181 gcctgcaagc atggtagcat ccatctcggg cggctgtcaa gcgccggctc gtgaggatgg
      241 cccgcgcgct gcacggtgcg tggatctttg cgattgcgct cccatacctt gtgaaccccg
      301 cccgcgcgca ggagttccgg gctctccccc agaaacatcc ggtagcgtga aggaacatcc
      361 gctgcatcac cggccgggtg gcgcagatgc cgctttgctt gctttcatga aggagaggta
      421 ctatggctgt tcagagagag atggatgctc cggactggca gaatgtgcag gtgttgagca
      481 tcaaccggga accggcgcac gcatcgctgc tgccttgccc ggatgaggag tgcgcgttga
      541 gcggcgagcg gggcgcttca ccctggttcc gtctcctgaa cggcaactgg cggttcatgc
      601 tcgtgcctta cccggacgca acgccggagg gatttgaggc ggaggagttc gacgcctcgg
      661 aatgggatac catccctgtg ccaagcaact ggcagatgca cggctacgat cggccccagt
      721 ataccaacat caactacccc tatcctatgg atcctccctt cgtgccagac gacaacccgg
      781 tggggctcta tcgccgcgcg ttcgtggtcc cggaagcatg gctgggccgg cgcatctatc
      841 tcaccttcga gggcgtcaac tcagccttct atctctgggt gaatggccat cgggtgggct
      901 atagccaggg aagccacatg ccggccgagt tcgacattac cccctacacc cgcctgggcg
      961 gcaacgtcgt cgccgtgcag gttttcaagt ggtcggatgc cagctatctg gaagaccagg
     1021 atttctggcg gctgagcggg atcttccgtg atgtctatct gaccgccaga agcgacgtct
     1081 acctgcgcga cgtcttcgtg cgcacgccgc tcgatgcaga gtatcgcgat gccaccctgg
     1141 aggtggatgt gagcgtgcgc aacgcggggg atgcgcccgt agccggctat gtggtctcag
     1201 ccaaactgat cgatccggac ggcgaggggg ccctcccgga gacgacggtc ggcgcggtcg
     1261 atctcgccgc gggcgaggag aaaaccctgg cgctgaaagc acctgtgagc gatccagaca
     1321 agtggacggc ggagacgccc tacctttact cactcctgct caccctgaaa gacgccaagg
     1381 gcgaggtgct ggaggtggag cacgtgcgcg tcggcttccg ccaggtggag attcgcgacg
     1441 ggcagttcct ggtcaatgga gtctccatca agctgcaagg cgtgaaccgg cacgatacgc
     1501 atccggatct aggccacgcg gtctcgctgg attccatggt ccaggatatc atgctgatga
     1561 agcagcacaa catcaactgc gtgcgcacct cgcactatcc caacgacccg cgctggtacg
     1621 acctgtgtga tgagtatgga atgtacgtga tcgacgaggc ggacctggag actcacggct
     1681 tcggctacga ggcgcccgat atccccgcca aacggcccga atggcgcgaa gcgttcctgg
     1741 atcgcgccgt gcgcatggtg gagcgcgata agaaccatcc ctgcatcgtg atgtggtcgc
     1801 tgggcaacga gtc
//
LOCUS       DAOSCV010000937         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43292_length_1811_cov_2.912301, whole genome shotgun sequence.
ACCESSION   DAOSCV010000937 DAOSCV010000000
VERSION     DAOSCV010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43292_length_1811_cov_2.912301"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..633)
                     /locus_tag="PLY56_17865"
     CDS             complement(121..633)
                     /locus_tag="PLY56_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23397.1"
                     /translation="MSDSHFLRSVARWLSVLMLAGTLCPRLPAWAADKPASDSVVARM
                     GAAVVDLSNNKVVLSAGERDGVVNGTIFRIMRHGEEIAQVKVFETSFATSNARVIRAG
                     QGAALRVNDRAEVLEIPVPRKRSGFRFPWAALVGAGIVALVVMGAVRDKGDGPSTGSP
                     TGGVAEINLN"
     gene            complement(652..1119)
                     /gene="ribE"
                     /locus_tag="PLY56_17870"
     CDS             complement(652..1119)
                     /gene="ribE"
                     /locus_tag="PLY56_17870"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOJ23398.1"
                     /translation="MPNTLEGKLNAQGLRFAIVVSRFNEFITGRLLSGALDSLIRHGA
                     DDSNITVAWVPGTFEIPIVAQKFAASGRFDAIVCLGALIRGATPHFDYLAAEVTKGIA
                     QIGLQTGVPTVFGVITTDNIEQAVERAGTKSGNKGFDAATTAIEMANLLRQLA"
     gene            complement(1243..>1811)
                     /gene="ribA"
                     /locus_tag="PLY56_17875"
     CDS             complement(1243..>1811)
                     /gene="ribA"
                     /locus_tag="PLY56_17875"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOJ23399.1"
                     /translation="RVRLPTPWGQFTAYGYECQLDHKPYVALVMGDISEGDILVRVHS
                     SCLTGDVFHSLRCDCGEQLQRALEVIAAEGRGVLLYIQQEGRDIGLINKLKAYELQEH
                     GKDTVEANEALGFAADLRDYGIGAQVLADLGLSRIRLMTNNPKKIAGLQGYGLQVVEQ
                     VPLPVTPTPDNRRYLKTKVEKLGHLIEL"
BASE COUNT          335 a          615 c          541 g          320 t
ORIGIN      
        1 gcagagggtc gccagggcga gggccccgcg ccaagagaat gggctcttct gaggctgcat
       61 actcccgtct cctcatgtga gcaggcgccg gcatcaagcc cctggaaagc agggcccggc
      121 tcagttcaga ttgatctccg ccacaccgcc ggtaggactg ccggtcgacg ggccatcgcc
      181 cttgtcgcga acggcgccca tgaccaccag cgccacgatt cccgccccca ccagcgcggc
      241 ccatgggaac cggaacccgg aacgctttcg tggcaccggg atctccaaaa cctccgcgcg
      301 gtcattgacg cgaagcgccg cgccctggcc cgcacgaatc actcgcgcgt tggaggtggc
      361 gaaggaggtc tcaaacacct tcacctgcgc gatctcttcg ccatggcgca tgatgcggaa
      421 gatggttcca ttcacgacgc catcgcgctc gcccgcgctc agcacgacct tgttgttcga
      481 caggtctact accgcggccc ccatcctggc gaccacggag tccgaggccg gcttatccgc
      541 ggcccaggca ggcagacgcg ggcaaagggt gcccgccagc atcagaacgc tgagccagcg
      601 cgccacggac cggaggaaat ggctatcgct catggcatac cccgttcagc ctcacgcaag
      661 ctggcgcagg aggttggcca tttcaatcgc ggtggtcgcc gcatcaaagc ccttgtttcc
      721 ggatttggtg ccagcccgct cgaccgcctg ctcgatgtta tcggtcgtga tcacaccaaa
      781 aacggtggga acgcccgtct ggagcccgat ctgcgcgatc cccttcgtca cctccgcagc
      841 cagatagtcg aagtggggcg tcgccccccg gatcagcgcg cccaggcaga cgatggcgtc
      901 gaagcgcccg gaagccgcga acttctgggc cacaatcggg atctcgaagg tcccgggcac
      961 ccaggccacg gtgatgttgc tgtcatccgc gccatggcgg atgagcgagt cgagcgctcc
     1021 cgacagaagc ctgccagtga tgaactcgtt gaagcggctg acgacgatgg caaatcgcaa
     1081 tccttgcgcg tttagcttgc cttctagtgt gttcggcacg ttctcgtatc ctcacccgat
     1141 ttgagatacc ggttgaatgg caagcctgcc cccggcaagg aggcaggagg cggtatcact
     1201 ccgttcgtta ttcgccagga ggacacgcct tccttctacg cgctatagct cgatcagatg
     1261 gcctagcttc tccaccttgg tcttgaggta gcgccgatta tccggcgtag gcgtcaccgg
     1321 aagcggcacc tgctctacca cctggaggcc atatccctgg aggccggcaa tcttcttggg
     1381 gttattggtc atcaggcgga tgcgcgaaag ccccagatcc gccagcacct gcgcgccaat
     1441 cccatagtcg cgcaggtctg cggcaaagcc cagcgcctcg ttcgcctcga cggtatcctt
     1501 gccatgctcc tggagctcgt acgccttcag cttgttgatc agcccgatat cgcgcccctc
     1561 ctgctggatg tagaggagca cgccccggcc ctcggcggcg atcacctcca acgcgcgttg
     1621 cagctgctcg ccgcaatcgc agcgcagtga gtgaaagaca tctccggtga ggcatgagga
     1681 gtgaacccgc accaggatat cgccctccga gatgtcgccc atcaccaggg ccacatacgg
     1741 cttgtgatcg agctggcact catagccgta ggcggtgaac tgcccccagg gcgtcggaag
     1801 gcgtacacgc g
//
LOCUS       DAOSCV010000938         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43318_length_1810_cov_3.765242, whole genome shotgun sequence.
ACCESSION   DAOSCV010000938 DAOSCV010000000
VERSION     DAOSCV010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43318_length_1810_cov_3.765242"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLY56_17880"
     CDS             complement(<1..422)
                     /locus_tag="PLY56_17880"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ23400.1"
                     /translation="MQVCVIGGTGHIGKNLVQMLLGEGYAVTVITTGRTPIPAGWESV
                     NVIQSAYRRGDAEWAAFLRDLAPEVLVDILGADVPGIYEAVKPSCQHLVVCGSLWMFG
                     EPRVVPTPEETQNPCGFEGYARRYEEMLATRERAQADG"
     gene            complement(728..1071)
                     /gene="rnpB"
                     /locus_tag="PLY56_17885"
     ncRNA           complement(728..1071)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLY56_17885"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1285..>1810
                     /locus_tag="PLY56_17890"
     CDS             1285..>1810
                     /locus_tag="PLY56_17890"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HOJ23401.1"
                     /translation="MIGLKRHTVRIVDYDPAWAARYREEARVIRSALDALAVDVQHVG
                     STAVPGLMAKPILDIAVAVEALEAIPEVVRRLVATGYIDRGDAGRNGGYLLARESEPE
                     VRVVHTHLVCRDDDQWRNYLAFRDLLRGDPEIRAGYARLKRELAARFPNDRRNYTAGK
                     EAFIRSALARAFSES"
BASE COUNT          299 a          571 c          609 g          331 t
ORIGIN      
        1 atgccatccg cctgggcgcg ctcccgcgta gccagcatct cctcataccg ccgggcgtag
       61 ccctcgaaac cgcacgggtt ttgcgtctct tcaggcgtgg gcaccacccg cggctcgccg
      121 aacatccaca gtgatccgca gaccaccagg tgctggcacg agggtttcac cgcctcatag
      181 atgcccggta catcggcgcc gaggatatcc accagcacct cgggcgccag atcgcgcaga
      241 aacgccgccc actccgcgtc gccgcgccga taggcgctct gaatgacgtt caccgattcc
      301 cacccggcgg ggatgggagt ccggcccgtc gtaatgacgg tgaccgcgta gccctcgcca
      361 aggagcatct ggaccaggtt cttgccgatg tggcctgttc cgccgatgac acagacttgc
      421 attctcaacc tcacaatccc gggactggct gcatgcggtg gcccctctcc cggaggtggg
      481 gcagcgggtg cttgctggtt gcaagcgcgc cctccaacga gcggattcgc gccagggccg
      541 ataaccgctc agtttgatcc gctgcagatc cgcggcttcg tgggagtgcc tttctcccgt
      601 tatcttgaga ggagctggag aggtggggcg attccggagt gcggcagcgt ggagagggag
      661 gtatccggaa gcgtatctga tcgagatgga agcagccatc tcgatgccgc gccacgtcac
      721 ctgcgtggga gggagtccgt ctataagccg agttctgtac cccttgcggg gcggcgatca
      781 tctgtctggg cgcaccggtc tcccgggcgc gcgatgcgct cagtttgggg caggacgggc
      841 cgccctattg ccccaggttg agcttgctcc aggcggggtt tgcccagccg atccgtctcc
      901 ggaccgctgg tggtctctta caccaccgtt tcagctttgc cggcagcgcc ggcgttttct
      961 tttctgtggc actttcactt caggttaccc tgacgggatg ttatccagcg ccttgccctg
     1021 cggagctcgg actttcctcg cccttacggg cgcgaccgcc caacggactc cctccgcagt
     1081 ctagattcta ccacaccccg cgtattgccg caaccaggag atcagcggtt gagacttcgg
     1141 aaggggcgag catcggatag ggccggctac tcgcagacac gctcggggag tgccaggatg
     1201 cggtttcttc gccggcgaag tgggagctat actcgggatg aacgccgatg gacgagatcg
     1261 gctggctgaa ctgggaggat ctaggtgatc ggtctgaaaa ggcacacggt ccgcattgtc
     1321 gattatgatc cggcatgggc tgcccgatat cgggaggagg cgcgcgtgat aaggagcgcc
     1381 ctggacgcgc tggctgtgga cgtgcagcac gtggggagca cggcggtgcc agggcttatg
     1441 gccaagccga tcctcgatat cgcggtggcg gtggaggcgc tagaagccat cccggaggta
     1501 gtgaggcgcc tggtggcgac cggctacatc gaccggggcg atgccggccg caacggcggc
     1561 tacctgctgg cgagggagtc ggagccggaa gtgcgggtcg tccacacgca cctggtgtgc
     1621 cgcgacgacg accagtggcg caactatctg gcgtttcgcg acctcctccg cggcgatcca
     1681 gagatccgcg cggggtacgc gcgcctgaag cgcgaactgg cggcccggtt cccgaacgac
     1741 cggcggaact acacggctgg caaggaagcg ttcattcgga gcgcgctcgc ccgggcgttc
     1801 tctgagagct
//
LOCUS       DAOSCV010000939         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43423_length_1807_cov_3.712900, whole genome shotgun sequence.
ACCESSION   DAOSCV010000939 DAOSCV010000000
VERSION     DAOSCV010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43423_length_1807_cov_3.712900"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1807)
                     /locus_tag="PLY56_17895"
     CDS             complement(16..>1807)
                     /locus_tag="PLY56_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23402.1"
                     /translation="QVEWRLACREHKAAQQKHDDLVAHHKRESEARAQAEAQARRWED
                     DRAAQEKAMASAARRRRSFSLAGGVLFLLAAVAGYALGQTWAPYCLIGAACLIAIAFG
                     TPAPVPLARQPLPPLPELTVTPEQLQNAAAELARAGEAANQVQTRLAEAAAAAQLPEE
                     VTEAVVEARRQELVREREEAQAAMVRRKELETRLQSAQARQKRAAEELERLEAEEHRA
                     QDATEKAQAAWGELLQSLGLPPEFQPGAARTYLDGARVVQARLREHDERSAEAKRLRE
                     RLERFREEAETLAATLGQEIPADDPWGLHAARQWRERLKEERENDLRRRNLLEKQREA
                     GEQCKQAEATADEAERQMEEVLRGAQAADENDLLRRVRDHEEWTRLTSELREVESQLR
                     STRSEEEWQEFLQRLESADWDALEQEAAKLTETEVALVQERDQAQQSIGSLQDAISKL
                     RQDEALAEAQARREEARARVVQLRRAWAPRTVAHALLTLTRRDFEAQRQPEAVRRTGA
                     LFEEVTGGRWGRIIASDGAVRVLREENGRVIERDEERLSRGARDALMLCARLAAIEQA
                     VDRLPLPVVLDDALVNLDRRRHERARSRRP"
BASE COUNT          211 a          678 c          593 g          325 t
ORIGIN      
        1 atccagacgg ctggatcaag gccggcgaga ccgcgcccgc tcgtggcgcc gccggtccag
       61 gttgaccagg gcatcatcca ggacgaccgg gagggggagg cggtccacgg cctgctcaat
      121 cgcggccagg cgcgcgcaga gcatcagggc atcgcgcgcc ccccggctga ggcgctcctc
      181 atctcgctcg atcactcgac cgttctcctc gcgcagcacg cggaccgcgc catcgctcgc
      241 gatgatccgg ccccagcgcc ctccggtgac ctcctcgaag agcgcgccgg tccgacgcac
      301 cgcttccggc tggcgctgtg cctcgaaatc gcgccgggtg agcgtcagca gcgcgtgtgc
      361 gacggtccgg ggggcccatg cacgccgaag ctggaccact ctcgcgcggg cctcctcgcg
      421 ccgggcctgg gcttccgcca gggcctcatc ctggcgcagc ttactgatcg cgtcctggag
      481 cgagccaatt gactgctggg cctggtcacg ctcctgaacg agcgcgacct cagtttcagt
      541 gagctttgcc gcttcctgtt cgagggcatc ccaatcggcg ctctccaggc gttgcaggaa
      601 ctcctgccac tcctcttcag agcgcgtgct gcgcagctgg ctctccacct ctcggagttc
      661 cgaagtgagc ctggtccact cctcgtgatc ccgcacgcgc cggaggaggt cattctcatc
      721 cgctgcctgc gccccccgga gcacctcttc catctgccgc tcggcctcat ccgctgtggc
      781 ttcggcctgc ttgcactgct ccccggcttc ccgttgcttt tcaagcaggt tgcgccggcg
      841 gaggtcattc tcgcgctcct ctttcaggcg ttctcgccac tgccgggcgg cgtgcaggcc
      901 ccaggggtcg tccgccggga tctcctggcc cagggtggct gccagagtct cggcttcttc
      961 acggaagcgc tccaggcgct cgcgcaatct cttcgcttcc gcgctacgct cgtcgtgctc
     1021 ccggagccgg gcctgaacaa ctcgcgcccc gtccaggtag gtccgggccg cgcccggctg
     1081 gaactcgggc gggagtccga gggattggag aagctccccc catgccgcct gggctttctc
     1141 cgtggcgtct tgcgcccggt gctcttccgc ttccaggcgt tccagctctt cggcggcgcg
     1201 cttctgccgg gcttgcgcgc tctggagccg cgtctccagc tctttacgcc ggaccatcgc
     1261 tgcctgcgcc tcttcgcgct ctcggacgag ctcctggcgc cgcgcttcga ccaccgcttc
     1321 cgtgacctct tctggcagct gggccgcggc cgccgcttca gcgagccggg tttgaacctg
     1381 attcgcggcc tcaccggcgc gcgcgagctc cgccgccgcg ttctgtagct gttccggggt
     1441 cacggtgagc tccggcaggg gcgggagcgg ctggcgcgcc aggggaaccg gcgccggagt
     1501 gccgaacgcg atggcaatca ggcacgcggc gccgatcagg cagtagggcg cccacgtctg
     1561 gcccagggcg tagcctgcca cggccgcgag gaggaacagg acgcctccgg cgagagagaa
     1621 gctgcggcgc cgccgggccg cgctcgccat cgccttctcc tgggcggcgc ggtcgtcttc
     1681 ccaccgccgc gcctgtgcct cggcctgggc gcgggcctcg ctctctcgct tatgatgcgc
     1741 gaccaggtca tcatgctttt gctgggcagc cttgtgctcg cggcaagcca ggcgccattc
     1801 gacttga
//
LOCUS       DAOSCV010000940         1802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43597_length_1802_cov_3.228392, whole genome shotgun sequence.
ACCESSION   DAOSCV010000940 DAOSCV010000000
VERSION     DAOSCV010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43597_length_1802_cov_3.228392"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PLY56_17900"
     CDS             complement(<1..1448)
                     /locus_tag="PLY56_17900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ23403.1"
                     /translation="MPVKLAPVQRQTLVERVRLVGVIAAERKATVAAQMPGRVVSVAV
                     QEGDAVAAGDLLVSLFDGDARMRVAAAEAEVRRARARMQSARTDTQIVSTEAHTTEAQ
                     AAEDLRIAEARLEQARSELRLAEERSGGEVAVAKAELEAAQSQLRLLQAGSRSEEVER
                     ARAEVEGAKVAVASARRDADQAKRDWERKRDLGERDFIPRRDVENAERDWQQAKAEVA
                     LAESRLRNLEAALRQLEAGPRPEEVAQARAQVQAAEERLRNATRNDALLAQKRQEIAA
                     AQAQVNRARKALEAARAADHRVRKSEHETRVVRSELEAAEANLRLARADLEKLQIYAP
                     MAATVASRDVNPGEMIQPGQALLTLIDTRSLYLNATVSDHDVSRIRVGQPVEVRFEAL
                     KGRVFPGQVQEILPPTSPQERNFHAKIRLERREPSIQTGMLATGSGIVARHQNVLTIP
                     EAAFLASAPDSHEGTVYVVEDGVARRRSVGVAA"
BASE COUNT          244 a          640 c          598 g          320 t
ORIGIN      
        1 gcggcaacac ccaccgagcg ccggcgggcc accccatcct cgaccacgta aacggtgccc
       61 tcgtgggagt ccggggcgct cgccaggaag gcggcttcgg ggatggtcaa cacattctga
      121 tggcgggcta cgatcccgct gccggtggcc aacatacccg tctggatcga cggctcccgc
      181 cgctccaggc ggatcttggc gtgaaagttg cgttcttggg gtgaggtcgg gggcaagatc
      241 tcctgcacct ggccgggaaa gacgcgcccc ttcagcgcct cgaagcgcac ctccaccggc
      301 tggcccaccc ggatccggga tacgtcatgg tcggagaccg tggcgttgag atagagcgac
      361 cgggtgtcga tcagggtaag aagcgcctgc ccgggctgga tcatctcgcc tgggttcaca
      421 tcgcgggagg cgacggtcgc tgccatcggc gcgtagattt gaagcttttc cagatccgcg
      481 cgcgccagcc ggaggttggc ttctgcggct tccagctcgg agcggacgac gcgcgtctcg
      541 tgctcgcttt tgcgaactcg gtggtccgcc gcgcgcgcag cttccagcgc cttgcgcgcc
      601 cggttcacct gcgcctgagc ggcggcgatc tcctgtcgct tctgggccag cagcgcatca
      661 ttgcgcgtgg cgttgcgcag gcgctcctcc gcagcctgca cctgggcgcg ggcctgtgcc
      721 acctcttcgg ggcgcggtcc cgcctccagc tgcctcagcg cggcctccag attgcgtagc
      781 cggctctcgg caagggccac ctccgccttc gcctgctgcc agtcgcgctc tgcattctcc
      841 acatcgcggc gagggatgaa gtcgcgctcg ccgaggtcac gctttcgctc ccaatcacgc
      901 tttgcctgat cggcatcgcg gcgcgcgctg gcgacggcga ctttggctcc ctccacctcg
      961 gcacgcgcgc gctccacttc ctcggagcgg ctgccggcct ggagcaagcg gagttgcgac
     1021 tgcgccgcct ccagctctgc ctttgccacc gcgacctcgc caccggatct ctcctccgcc
     1081 aggcgcaact cactgcgcgc ctgctccagg cgggcttcgg cgatgcggag atcctcggct
     1141 gcctgggctt ccgtggtatg agcctccgtg ctcacgatct gcgtatcggt gcgtgcgctc
     1201 tgcattcgcg cgcgcgcccg gcgaacctct gcctccgccg cggccacgcg catccgcgca
     1261 tcgccatcga agagcgatac cagtaggtcg cccgcggcca ctgcatcccc ttcttggacc
     1321 gcgaccgaga ccacacgtcc cggcatctgc gcagccacgg tggccttgcg ctcggcagca
     1381 atgactccaa cgaggcgcac tcgctctacg agagtctgcc gctgcaccgg ggcgagcttg
     1441 acgggcacgg ccgtccgctc atccgcacgt tcggaagccg gaggagcgga tgcttgagca
     1501 gtggtgacag gaggcgtcgg agggcggcgc gtgcatccgg cagccagcaa taggatgagg
     1561 gttgccaggc cgatgggata ccgttgtgcg ctcacgtgat tgccctatct ctgctctcgc
     1621 cagttcgtgg taccgttctg catgcgcgcc ccccttccct ctttgggatc gtgaggcagc
     1681 cacggggatg ggcgctctcc ctgcgcagcg accgcacgct ggacagagga tcgggccgac
     1741 gggttctccg gcctgcccgc gggccgggtg gtgagattcg gagaggacgg actggtctac
     1801 cg
//
LOCUS       DAOSCV010000941         1800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43666_length_1800_cov_2.959312, whole genome shotgun sequence.
ACCESSION   DAOSCV010000941 DAOSCV010000000
VERSION     DAOSCV010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43666_length_1800_cov_2.959312"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..204
                     /locus_tag="PLY56_17905"
     tRNA            128..204
                     /locus_tag="PLY56_17905"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:162..164,aa:Asp,seq:gtc)
     gene            246..321
                     /locus_tag="PLY56_17910"
     tRNA            246..321
                     /locus_tag="PLY56_17910"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:279..281,aa:Phe,seq:gaa)
     gene            complement(506..1015)
                     /locus_tag="PLY56_17915"
     CDS             complement(506..1015)
                     /locus_tag="PLY56_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23404.1"
                     /translation="MRAAAPRPIVVADACVLIDFLGAAEELLVLGARRVWEVHVLTPI
                     VAEIRSLEEGRCDELGLVAVEPEFDLLLEAQTRRRSLSFQDRLWLVYAKNLTPNGVVW
                     TNDKKMRQEAEADGLQVYWGLDLLVELVRQGHAEQEEALDAASAILANARFKVEPIMA
                     QFRAALAAL"
     gene            complement(1012..>1800)
                     /locus_tag="PLY56_17920"
     CDS             complement(1012..>1800)
                     /locus_tag="PLY56_17920"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOJ23405.1"
                     /translation="DWKLQELVGRHDPITAASEVRRVAGIGDEPVRDVCGLLEYLGVK
                     VLSRPVCTDGFFGLSVGPADGGPAIVVNTWERIPVERWIFSAVHELGHLVLHPDSFRV
                     EQADEIAQEEKEADRFAAHFLMPFAVFKQEWDDTCGLPLIDRVLKVKRIFRVSYKTVL
                     YRLAEEGDRSVWQRFNAQYARHYGRRARHSEPLPLGPEEYQPVENRRSKEPEQLVDVD
                     FTADRLSRLVRRALEEERITRSRAAEVLRLSHEELLERMEDWAA"
BASE COUNT          318 a          570 c          588 g          324 t
ORIGIN      
        1 ggggagggtt cctcggtggt caggccggtg ggcaaaaaaa agcagctcgc cttcgcaaaa
       61 atcgcttcca gaacctattg acagaaatcg agagttgcga tatactatgg gtgcgattgg
      121 gggataagga ccagtagcgt agcggtttaa cgcgcatgcc tgtcacgcat gagatcgcgg
      181 gttcgaatcc cgtctggtcc gccagtcgat gggggccgtc caagaggatg gcccccatct
      241 cgcttgccga gatagctcag ttggtagagc acacgactga aaatcgtgtt gtccccagtt
      301 cgattctggg tctcggcacc agaatgcctc ttcgggccgg acgcggtgct ttccgtgtcc
      361 ggcccttctc ttttgccatc gccacctgct ctcttccgct tgaagcgggc cgttcagact
      421 ggcaggaggg atggtccggc gtggccggcg ccgggcatgc cccgaacgcc aggtggtgac
      481 ggcagaaacc cagggggacg cggcttcaga gcgccgccag tgcggcacgg aactgcgcca
      541 tgatgggctc taccttgaac cgcgcgttgg cgaggatcgc tgaggctgcg tcgagcgcct
      601 cttcctgctc cgcgtgtcct tgccgcacca gctccaccag cagatcgaga ccccagtaaa
      661 cctgaagccc atcggcctcg gcttcctggc gcatcttctt gtcgttggtc cacacgacgc
      721 cgttgggtgt gaggttcttc gcgtagacca gccagaggcg gtcctggaag gagaggctcc
      781 gccggcgggt ctgagcctca aggagcagat cgaactccgg ctcgacagca accaggccca
      841 gctcatcaca gcgcccctcc tctagggaac ggatctcggc gacgatgggc gtcagcacgt
      901 ggacctccca gacgcgacgg gcgccgagca ccagaagctc ctccgccgcg ccgaggaaat
      961 cgatgaggac gcaggcgtct gcgacgacga tgggcctggg tgctgccgct ctcatgcggc
     1021 ccagtcctcc attcgctcga ggagctcctc gtgggagagg cgcaacacct cggcggcgcg
     1081 ggagcgggtg atccgctcct cctcgagcgc gcgtcgcact aggcggctga gccgatccgc
     1141 ggtgaagtcg acgtctacga gctgctcggg ctccttggaa cgcctgttct caaccggctg
     1201 gtactcctct gggccgagcg gcaggggttc gctatgccgg gcgcggcggc cgtagtgacg
     1261 cgcgtactgt gcattgaacc gttgccatac gctcctatcg ccctcctcag ccagccggta
     1321 gagtacggtc ttgtagctca cccggaagat tcgcttgacc ttgagcaccc tgtcgatgag
     1381 gggaaggcca cacgtgtcgt cccactcctg cttgaagacc gcgaacggca tgaggaagtg
     1441 ggcggcgaag cgatcggcct ccttttcttc ctgggcgatc tcgtcggcct gctccacacg
     1501 gaaggagtcg gggtgaagga cgagatgccc gagctcgtga acggcgctga agatccagcg
     1561 ttccacgggg atgcgctccc acgtgttaac gacgatggcg gggccgccgt cagcgggccc
     1621 cacggagagg ccgaagaagc catcagtaca gactgggcgg gagagcacct tgaccccaag
     1681 gtactcgagc aggccgcaga catcgcgcac gggctcgtca ccgatgccgg ccactctcct
     1741 gacttcggat gccgcggtga tcgggtcgtg acggcccacc agctcctgga gtttccaatc
//
LOCUS       DAOSCV010000942         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43744_length_1798_cov_2.756741, whole genome shotgun sequence.
ACCESSION   DAOSCV010000942 DAOSCV010000000
VERSION     DAOSCV010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43744_length_1798_cov_2.756741"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1454)
                     /locus_tag="PLY56_17925"
     CDS             complement(<1..1454)
                     /locus_tag="PLY56_17925"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ23406.1"
                     /translation="MRCFTLRRYFPLLTLALLYALPAAAEPAIETGVPAAPLGLRIID
                     LAPDRISLDWLPPEKPVAGYRLYRDGRRIAELPGDALSYVDTGLKPSTLYIYRLTAFG
                     AGDLPLESDWSQPAADFTLPPATANAPRGNEYDVVVVGATPGGIGAALAASRLGATVA
                     LVEPSRWIGGMMAGGLSNTDYRFKETHGSVVKEFAGQVLDYYRRVYGPDSRQVRVSIG
                     GYKFEPRVARAVLTRMIAQEPRISLYRQWRATGVEKEGNRVTAVTFEDLARGGMHTLR
                     GKFIIDATYEGDIAAYAGAEYRVGRESRDDYNERHAGEIFWNLDEKKIVWGSGAGDRR
                     VQAYNYRLCMTTRPDLRLPAKRPEGYDRTHYLKIIGDARSGYATVKRVLGLQGALPND
                     KWDINNSGGYWPSTDFVGANYDYPEADWQRREEIIRAHRAYVQGLLYFIQNDPELPEE
                     FRKEANRWGLCRDEFVDNDNWPTQLYIREGRRIVG"
     gene            complement(1593..>1798)
                     /locus_tag="PLY56_17930"
     CDS             complement(1593..>1798)
                     /locus_tag="PLY56_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ23407.1"
                     /translation="LESKLSEREREVLKLIAEGYTNKEIADMIFLSVKTVETHKAHIM
                     DKLELRSRAELVRYALDNGLLKP"
BASE COUNT          294 a          569 c          618 g          317 t
ORIGIN      
        1 cccacgatcc ggcgcccctc ccggatgtag agctgcgtcg gccaattgtc gttgtcgacg
       61 aactcgtcgc ggcagagccc ccagcggttg gcctccttcc ggaactcctc gggaagctcc
      121 gggtcgttct ggatgaagta gaggaggccc tggacatagg cgcggtgcgc ccggatgatc
      181 tcctcgcgcc gctgccaatc cgcctcgggg tagtcgtagt tggcgccgac gaaatcggtg
      241 gagggccagt agccgccgct gttgttgata tcccacttgt cgttggggag agcgccctgc
      301 aagccgagga cgcgcttcac ggtggcgtag ccggagcggg catcgccgat gatcttcagg
      361 taatgcgtgc ggtcgtagcc ttccgggcgc ttggcgggaa ggcggagatc cgggcgggtg
      421 gtcatgcaga ggcggtagtt gtaggcctgc acacgacggt cgccagcacc gcttccccac
      481 acgatcttct tctcgtcgag gttccagaag atctcgccgg cgtggcgctc gttgtagtcg
      541 tcgcggctct cgcggcccac gcggtattca gcgccggcat aggccgcgat gtcgccctca
      601 taggtggcat cgatgatgaa cttgccgcgc agggtatgca tgcccccgcg ggccagatcc
      661 tcgaaggtga ccgcggtgac gcggttgccc tctttctcca ctccggtagc tcgccactgt
      721 cggtagagag agatgcgcgg ctcctgggcg atcatgcgcg tgagcacggc gcgtgcgacg
      781 cgtggctcga acttgtagcc gccaatcgaa acacggacct gccgggaatc ggggccgtag
      841 accctgcgat agtagtcgag cacctgcccg gcaaactcct tgacaacgct gccgtgcgtc
      901 tccttgaagc ggtaatcggt gttgctgagg ccacccgcca tcatcccgcc gatccagcgc
      961 gacggctcga caagagcgac ggtggcaccg aggcgagacg ccgccagcgc cgccccgatc
     1021 cccccgggcg tggcgcccac caccaccacg tcatactcat tgccccgggg ggcgttcgcc
     1081 gtggcgggtg gaagcgtgaa atcggcggcg ggctggctcc aatcgctctc caggggtagg
     1141 tcgcccgcgc caaacgccgt caggcggtag atgtagagcg tcgagggctt caggcccgta
     1201 tccacatagc tcagggcatc tcccgggagt tcggcgatcc gtcggccatc gcgatagagg
     1261 cggtagccgg ccaccggctt ctccggaggc agccaatcga ggctgatgcg atcaggagcc
     1321 aggtcgatga ttcgcaatcc gagaggggct gcgggcaccc ccgtctctat cgcgggctcc
     1381 gccgcggcag gcagggcata caggagagcg agggtgagca acgggaagta tcgtcgcaaa
     1441 gtaaagcagc gcatgcgatg gatgcctttc catggcgccg gcggacggag ccgtgcgtcc
     1501 ggcaagggag agggggagaa gcgctgtggc ggcgcaagga gtgcgagctg ccgggctctc
     1561 gcggatgagc gcgagtggcc acccgggccg ggtcacggtt tgagcaggcc gttatcgagc
     1621 gcgtagcgca ccagctccgc gcgactgcgc agctcgagct tatccatgat gtgcgccttg
     1681 tgcgtctcca cggttttcac gctcaagaag atcatatcgg cgatctcctt gttggtgtag
     1741 ccctcggcga tcagcttgag cacctcgcgc tcgcgttcgc tcagtttgct ctccaggc
//
LOCUS       DAOSCV010000943         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43799_length_1796_cov_3.616312, whole genome shotgun sequence.
ACCESSION   DAOSCV010000943 DAOSCV010000000
VERSION     DAOSCV010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43799_length_1796_cov_3.616312"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1147)
                     /locus_tag="PLY56_17935"
     CDS             complement(<1..1147)
                     /locus_tag="PLY56_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23408.1"
                     /translation="MVPLVRQILLLPALLLLLSTSGFAQQTPDHERTLLFLSPSGTLY
                     MANISTQGVRIPQASLAPGKQVRRFRVSPDGRIVLLETANTKGVLSVWMLELSSRRSR
                     LLGQGLLVSDDGSSPFAPDGRRVALRHQNRDEVMIFSANTGQVLRTLSIPGIREAPAW
                     FGDARHIAVPTQLPTGRSAIAVWDAVSGSLRREAAKDVAQVRFRYETRLDGRLIYRET
                     REGVDRWLALTTTGETAVDEPPAPRLPQDLPDGASAAACPEGPWLAYIHEKSLYVRYS
                     EEGAEPVMVAPAVECHWKPGKPLPEQPLTAPATAPAGSAAPAVLPNVLALKTETDEAV
                     EGTRLEITWESEAQVAWVRLSAVAERARVGGARRGVQTVPIARVPAAS"
     gene            complement(1484..>1796)
                     /locus_tag="PLY56_17940"
     CDS             complement(1484..>1796)
                     /locus_tag="PLY56_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23409.1"
                     /translation="IVLGDMAPDKYKYDPRVIYVPGWPVPQSYIQHEMVDGMGFGHLY
                     APGLRLFEEGASLIAQLRGASGPEVQRRAAAKVAGAAVLAAGIPLALRALFRSDRAGK
                     K"
BASE COUNT          305 a          596 c          608 g          287 t
ORIGIN      
        1 tcgacgctgc cggaactcgc gcgatgggta cagtctgaac accgcgcctc gctccgccca
       61 cgcgagcgcg ctctgcgacg gcgctcagcc gaacccatgc cacctgtgcc tcggactccc
      121 aggtgatctc caggcgggtg ccctcgaccg cctcgtccgt ctccgtcttc agcgcgagca
      181 cgtttgggag caccgccggc gcggcacttc ccgctggcgc cgtggctgga gcggtgaggg
      241 gctgctccgg gagcggcttc cccggcttcc agtggcattc taccgcaggg gcgaccatca
      301 ccggctccgc gccttcctcg ctgtagcgca catagagcga tttctcatgg atgtaggcga
      361 gccacggacc ctccgggcag gccgccgcgc ttgcgccgtc aggaagatcc tgcggcaggc
      421 gcggagcagg gggctcgtct accgcggtct cgcccgtggt agtgagggcc agccagcggt
      481 cgacgccctc gcgggtttca cggtagatga gccggccatc aaggcgcgtc tcgtagcgga
      541 agcgcacctg cgccacgtcc tttgccgcct cgcggcgcaa agaaccggag acggcgtccc
      601 aaacggcaat ggcgctgcgc ccggtgggca actgagtggg aaccgcgatg tgccgggcgt
      661 cgccaaacca cgccggcgcc tcgcggatcc ccgggatcga gagggtgcgc aacacctgac
      721 cggtgttcgc ggagaagatc atgacttcgt cacggttctg atggcggagt gccacccgcc
      781 ttccatccgg cgcgaagggg ctggagccgt cgtcggagac gagcaagccc tggcccagca
      841 gtctcgaccg ccggctggag agctccagca tccagaccga aagcactccc ttcgtgttgg
      901 ccgtttccag gagcacgatg cgtccatcag ggctgacgcg gaagcggcgc acctgctttc
      961 ccggcgcgag tgacgcctgc ggaatgcgca ctccctgggt cgagatgttc gccatgtaga
     1021 gggtgccgga tggactcagg aagagcagcg tccgttcgtg atccggggtc tgttgagcga
     1081 agccagatgt gctgagaagt aaaaggagag cgggtaggag caatatctgc cggaccaagg
     1141 gtaccaagac gtgtgtgacc tcctcgcaca ggtgcttcca gccataatca tcggcattgt
     1201 tcgctgcaac gagaggggct tcctttcagt gggcagcgcc agcagtaggg acaccgggcg
     1261 ttgggcgagg tgggacgccg cttccccccc ccccggcagg ttcacccccg gagatcgttc
     1321 gacctcagtg agcgcggcgg tagaaaaggc gcttgccgca cgcgggtccg cgatcgccca
     1381 aacggcgctg aaaagtgcgc gcggcaagcg cccggaggcc cgcccaggaa ggagtcccgt
     1441 ggcagcctcc ctgatgaatc cggcttggtg acgaccggaa tcgctacttc ttgcctgctc
     1501 gatctgaacg aaagagggcg cgtagggcga gggggatgcc ggccgccagc acggcggccc
     1561 ccgccacctt cgccgcggcc cggcgctgta cttcggggcc agacgcgcct cgcagttgtg
     1621 cgatcaacga cgcgccttcc tcaaaaaggc gcaaccccgg cgcatagagg tgcccgaagc
     1681 ccattccgtc gaccatctcg tgctggatgt aggactgggg caccggccag ccgggcacgt
     1741 agatgacacg cgggtcatac ttgtacttat ctggtgccat gtctccgagg acgatg
//
LOCUS       DAOSCV010000944         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43800_length_1796_cov_3.522114, whole genome shotgun sequence.
ACCESSION   DAOSCV010000944 DAOSCV010000000
VERSION     DAOSCV010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43800_length_1796_cov_3.522114"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            662..1735
                     /gene="rfbB"
                     /locus_tag="PLY56_17945"
     CDS             662..1735
                     /gene="rfbB"
                     /locus_tag="PLY56_17945"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOJ23410.1"
                     /translation="MRILVTGGAGFIGSNFLNLMVPRHPEHHFINLDKLTYAANLASL
                     EAICDAPNYTFVQADIADARQVDQVFDAHDPEWVFHFAAESHVDRSILGPAAFVQTNV
                     IGTFVLLEACRARWQGRVGRLFHHVSTDEVYGSLGDTGAFTEETRYDPSSPYSASKAA
                     SDHFARAYHRTYGLPVKVTNCSNNYGPRQFPEKLIPLMILNALEGKPLPVYGQGTNVR
                     DWLYVEDHCEAIWLVATRGRVGETYHIGGGTEKRNLDVVHTICQLVAEETGRDPDSLR
                     ALITYVADRPGHDFRYAMDTTKIRRELGWEPRESFETGLRKTVRWYLNHRAWVEQVRS
                     GEYARWLERNYAGREAHGKDAGR"
     gene            1732..>1796
                     /locus_tag="PLY56_17950"
     CDS             1732..>1796
                     /locus_tag="PLY56_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ23411.1"
                     /translation="MTRKGIILAGGSGTRLYPLTHV"
BASE COUNT          323 a          580 c          567 g          326 t
ORIGIN      
        1 cgccggggca tatcgcacgg gcgtgagcat ccgttccgct taggggcaaa gccctacggg
       61 cagggggtga tctgcgcccc gctctccttg acaaagacct gaccgcatgc tatacttcaa
      121 cctggtcagg atggccgcgc ggagcaatcg ccggccttca tagctgcgcg agcggcgtcc
      181 ggtgccaccg cgaccgtgcc gaaggagtga agaattacct tgcacttctt caccatcaac
      241 aggtcgcccc atcatcccgg tcgtgagtgc agtgtcatcc ttctccccgg caagcggccg
      301 gcgccgattc ttcggaaagt gccccgaact gtggcgtgag tgtccggtgg tgggaaggca
      361 acgctttgtc tgccatttcc cagcgaggat ccagcggaag ctttccagca gttgcctcga
      421 ggctctcccg cccaccacgc gagaccaaca ccccggtgaa gcaacggttt tgctccccag
      481 atgcccctgg gcggtccgtt cccgggttca gcgaatcggg ctcaactgaa ccagatgagg
      541 ccgcaggcgc tccttgtagc gggagacctc gttcgcggca ccgcggcgcc ggcggcagga
      601 agcgagtggc tctcccaaat ccttggactt ggccgtcttc aaccggcgcg gggaggtagt
      661 cgtgcgcatt ctggtgaccg gaggcgctgg gttcatcggg tccaacttct tgaacctgat
      721 ggttccccgc catcctgagc atcattttat taatctcgat aaactgactt acgcggccaa
      781 cctcgccagc ctggaggcga tctgcgacgc tcccaactac accttcgtgc aggcggacat
      841 tgccgatgcc cgccaggtgg accaggtgtt cgacgcgcat gacccggagt gggtcttcca
      901 cttcgccgct gaaagccacg tggaccggag catcctcggc ccggcggcgt ttgtgcagac
      961 caatgtgatt ggcacttttg tgcttctgga ggcgtgtcgc gcgcgctggc aaggccgtgt
     1021 cggccgcctc tttcaccacg tgagcacgga tgaggtgtac ggctcgctgg gcgataccgg
     1081 cgccttcacc gaagagacgc gctacgatcc cagcagcccc tactccgctt ccaaggcggc
     1141 gagcgaccac ttcgcgcgcg cctaccaccg cacctacggg ctccccgtga aagttaccaa
     1201 ttgcagcaac aactacggcc cgcgccagtt cccggagaag ctgatcccac tgatgattct
     1261 gaacgcactc gaggggaagc cgctgccggt gtacggtcag ggcaccaacg tgcgcgattg
     1321 gctctatgtg gaagaccact gcgaggcgat ctggctcgtc gccacgaggg gtcgcgtggg
     1381 cgagacgtat catatcggcg gcgggacgga gaagcgcaat ctcgacgtgg tgcataccat
     1441 ctgccagctg gtcgccgagg agacggggcg cgatcccgat tccctgcggg cgctcatcac
     1501 ttacgtcgcc gaccgcccgg ggcatgattt ccgctacgcg atggatacca cgaagatccg
     1561 ccgcgagttg ggctgggaac ctcgggaaag ttttgagacc gggctgcgca agacggtccg
     1621 ctggtacctg aaccaccgcg cctgggtcga acaggtccgc tccggggagt acgcccgctg
     1681 gctcgagcgc aactatgccg ggcgggaagc tcacggaaag gatgcgggtc gatgacacgc
     1741 aaggggatca tcctggcggg aggctcggga acccggcttt accccctcac gcatgt
//
LOCUS       DAOSCV010000945         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_43836_length_1795_cov_3.891954, whole genome shotgun sequence.
ACCESSION   DAOSCV010000945 DAOSCV010000000
VERSION     DAOSCV010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43836_length_1795_cov_3.891954"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1795
                     /locus_tag="PLY56_17955"
     CDS             <1..>1795
                     /locus_tag="PLY56_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23412.1"
                     /translation="NDEVVAQGPASAGTEPIQIPLKKGPNVFALRLEKGTAAMRLEAP
                     GWKGEAVRWRMAPASTRDATLATTDDSTWAIAPKAGDHPKLGAVLGDPGKPVILRHTL
                     LWEKTRTWPTPSPALYIARDSAQHLTFIADGLPGRKLVDWSVTLAVPPAFEILGATGY
                     YGERDPQPIFRCTQLGEREVDGKKMRLARITADKPVLSGRHYIMSLFNAFVRTRPGAS
                     LEGNETRFLYWSEANDGTVTEPVQEIPVRILPPLKGKQPKQLVWQLWGSFFGNMDDPA
                     MREETLRTAQAAGFNDIVSGSRWTSDNAPRFGMTHTLGIKFQSWSINLGPYLEAHPNE
                     CLIDREGKPSKSLLCTTLLLDQSWPAVEAALKERLDEARPHIVDYDFEYNPFDGPHSC
                     YCQRCLKAFREFARIPAETPLDAATIKEKHDEAWTDFMARRVAQVFAKLKDGVRRVAP
                     GTKLSAYSGYQTPDNPRTYGVNWAYFGELKAADRAGCGYGRPVEHIPGTIQALNGIPV
                     LFGALMTPYDTSITAPQVPIPKARLLRRSLDSTGGVLVYDRLPMDGRTWLAVAETTRL
                     VAEFEPLFLTGKRSALQGHDPAVVQLLSDGKT"
BASE COUNT          322 a          634 c          572 g          267 t
ORIGIN      
        1 caacgatgaa gtggtggcgc agggcccggc atcggccggc actgaaccga tccagatacc
       61 tctgaagaag ggcccgaacg tctttgcgct gcgactcgag aagggcacgg cggccatgcg
      121 tctggaagcc cccggctgga agggcgaagc cgtccggtgg cggatggcac ccgcgagcac
      181 ccgcgacgcc accctggcca cgaccgatga cagcacctgg gccatcgccc cgaaagcggg
      241 cgatcatccg aagctgggcg ccgtgctcgg cgatcccggg aagccggtca tcctccggca
      301 cacgctcctg tgggagaaga cgcgcacctg gcccacgccg tctccggcgc tctacatcgc
      361 ccgagatagc gcacagcacc tcacgttcat cgcggatggc cttcccggac ggaagctggt
      421 ggactggagc gtcaccctcg cggtgccgcc cgcgttcgag atcctcggcg cgaccggcta
      481 ctacggcgag cgggatccgc agcccatttt ccgctgcacc cagctcggcg agcgcgaggt
      541 ggacggcaag aagatgcggc tggcgcggat caccgcggat aagccggtcc tctctggccg
      601 gcactacatc atgtcgctct tcaacgcctt cgtccgcacg cgcccgggcg cttccctgga
      661 agggaacgag acgcgcttcc tctattggag cgaggcaaac gacggcaccg ttaccgagcc
      721 tgtccaggag attccggtgc gcatcctacc cccactgaag gggaagcagc cgaagcagct
      781 cgtctggcag ctgtggggct cgttcttcgg caacatggac gacccggcga tgcgcgagga
      841 gacgctgcgc acggcgcagg ccgccggctt caacgatatc gtctccggca gccggtggac
      901 cagcgacaac gcgccccgct tcggcatgac gcataccttg ggcatcaaat tccagtcctg
      961 gtcgatcaac ctggggccct acctggaggc ccacccgaac gagtgtctca tcgatcgcga
     1021 gggcaagccg agcaagagtc tcctctgcac cacactcctt ctcgatcaat cctggcccgc
     1081 ggtcgaggcc gcgctgaagg agcggctgga cgaggcccgt ccgcacatcg tggactacga
     1141 cttcgagtac aacccctttg atgggccgca ctcctgctac tgccagcgct gcctgaaggc
     1201 gttccgcgag ttcgcgcgca tcccggcgga gacgccgctc gatgccgcca cgatcaagga
     1261 gaagcacgac gaggcctgga ccgactttat ggcgcgccgc gtcgcgcagg tcttcgcgaa
     1321 gctgaaggac ggcgtccgcc gggtggctcc cggcacaaaa ctgagcgcct acagcggcta
     1381 ccagacgccg gacaacccgc gcacctacgg tgtcaactgg gcctacttcg gcgagctgaa
     1441 agccgccgac cgggccggat gcggctacgg ccggccggtg gagcatatcc ccgggacgat
     1501 ccaggcgctc aatgggatcc cggtcctctt cggcgccctg atgaccccct acgacacgag
     1561 tataaccgcg ccacaggtgc cgattcccaa ggcgcgcctg cttcgccgct cgctcgactc
     1621 gaccggaggc gtgctggtct atgaccgcct ccccatggac gggcgtacgt ggctggccgt
     1681 cgcggaaacc acgcggctcg tcgctgagtt cgaaccactc ttcctgaccg ggaagcgcag
     1741 cgccttgcag gggcacgacc ccgccgtcgt acaactcctc agcgatggca agacg
//
LOCUS       DAOSCV010000946         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44238_length_1783_cov_3.634259, whole genome shotgun sequence.
ACCESSION   DAOSCV010000946 DAOSCV010000000
VERSION     DAOSCV010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44238_length_1783_cov_3.634259"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLY56_17960"
     CDS             complement(<1..491)
                     /locus_tag="PLY56_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23413.1"
                     /translation="MRRTHLSEEAGARAEDGSALIVTLALVLALTILVAATQWQVIHD
                     LKVSKTERDFERALQMAESGANAYLNMLAKGLAPGNPLVPQQYVLPSVLSPQEFKQQV
                     KNKTIPPSHLVYYPPGQTRQGYFAAHLGTPGTSADIIAYGWSNGIVRRVRIHSDIYSI
                     FDWV"
     gene            complement(924..1397)
                     /locus_tag="PLY56_17965"
     CDS             complement(924..1397)
                     /locus_tag="PLY56_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ23414.1"
                     /translation="MKHSTRKTQGFSLVELLAVVLVLAVLAAVAIPLYLNTRKSAAAR
                     TCKANLTALAAAEAAFVVRHGRFNTTPMVDYNATDPLSGGLVGAPEGITSPLNCPWDG
                     AAYTIAGGGVGEPLTISCPHCATAKGGDNAHEAISPSPDGFSFYYRTLSAPATEQ"
     gene            complement(1437..>1783)
                     /locus_tag="PLY56_17970"
     CDS             complement(1437..>1783)
                     /locus_tag="PLY56_17970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23415.1"
                     /translation="LHLRPVIKARDCKERIEALARSNAAFVLHDGRLVYAPQLAYTGA
                     AEALGGILARLEGLPARPTCPLDGTPYDVLSDSAGHVIIRCSNARKHASVLPVLKDYI
                     RRGLSAQPEYLP"
BASE COUNT          327 a          538 c          592 g          326 t
ORIGIN      
        1 acccagtcaa agatgctata gatatccgag tggatgcgca cgcggcgcac gatgccgttg
       61 ctccacccat aggcaatgat atcggcgctc gtgccgggcg tgcccagatg ggccgcaaag
      121 tagccctgcc gcgtctggcc aggggggtag tacaccaggt gggaaggggg gatggtcttg
      181 ttcttcacct gctgcttgaa ctcctgtggc gacaggacgc tcggaagaac gtattgctgg
      241 ggcaccaacg ggttgcccgg agccagacct ttcgccagca tattcagata ggcgttggcc
      301 ccagactctg ccatttgcag ggcgcgctcg aagtcgcgct ccgtttttga gaccttcaaa
      361 tcgtggatca cctgccactg agtggcggcc acaaggatgg tgagcgccag caccaacgcc
      421 agcgtcacga tcaaagcaga gccgtcctct gcacgcgctc ctgcctcttc actgaggtgc
      481 gtacgtctca tgactggtct ctccctctgc ttaggggcct gatcgccagc tcctggccgg
      541 cgtgcttgaa ggaagcatcc cctaggtgct gcaaccgcat cataccccta cagcgcgttc
      601 tctattccac caaaagcagc tgagaggggg ctcttttctg gcgcgggcgt ggataccccg
      661 ggtgaaaggg acggacgtgg ctgggaaagc gcaggcattt cgggctaagt tgtacgatcc
      721 cttcggggtc gacggacaac atgcaccgcg ctgatggagg aacccttggg cgaaacgtcc
      781 accgcgcagc gctctcggag gaccccgagg gcggccggta ttcgagctcc gggtctcctg
      841 accctgggca tagtggcgga tggggtgaca ctctcggcgg tggaatcggc agaggggcgc
      901 ggacgcgccc cctgtcgatt gcatcattgc tcggtggctg gggcggatag cgtgcggtag
      961 tagaagctga agccgtcggg gctgggtgag atcgcctcgt gggcgttatc cccgcccttg
     1021 gccgttgcgc agtgcgggca ggagatggtc agcggttcgc ccactccacc gcccgcgatc
     1081 gtgtaggccg ctccgtccca agggcaattg agcggcgagg tgattccctc cggtgcgccc
     1141 accaatccgc cagagagtgg atccgtggcg ttgtagtcta ccatgggcgt ggtgttgaaa
     1201 cgaccgtggc ggaccacgaa cgccgcctca gctgcagcga gcgccgtcag gttcgccttg
     1261 caggtacgtg ccgcggccga cttgcgcgtg ttgagataga gcggaatggc cacggcggcc
     1321 aataccgcca gaaccagcac caccgcgaga agctccacca gggagaaccc ctgggtcttc
     1381 cgggtagagt gtttcatgga agcatcctcc tcctccgcgg ggcaagctcc acaatcttat
     1441 gggagatact ctggctgggc cgacaggcct cggcggatgt aatccttcag aactggcaga
     1501 acactggcgt gtttccgggc attggaacag cggatgatca catgcccggc gctatcactc
     1561 aggacatcat agggggtgcc atccagtggg caggtggggc gggccggtag accctccagc
     1621 cgggccagaa tgccaccgag ggcctccgcg gcaccagtgt aagccaattg gggcgcgtaa
     1681 acgagccggc cgtcatgcag gacgaacgcg gcgttcgatc gggcgagcgc ctcgattcgc
     1741 tccttacaat cgcgggcctt gatcaccggg cgcaggtgaa ggt
//
LOCUS       DAOSCV010000947         1783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44246_length_1783_cov_3.086806, whole genome shotgun sequence.
ACCESSION   DAOSCV010000947 DAOSCV010000000
VERSION     DAOSCV010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44246_length_1783_cov_3.086806"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLY56_17975"
     CDS             complement(<1..948)
                     /locus_tag="PLY56_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ23416.1"
                     /translation="MARKRVTIDGNEAAAYVAYKTNEVMTIYPITPSSPMGELGDAWA
                     AAGQTNIWGQVPLVQEMQSEGGAAGAVHGSLQAGALTTTFTASQGLLLMIPNMYKIAG
                     ELTPTVFHITARTLATHALSIFGDHSDVMACRATGWAMLFSNSVQEVMDFALIAQAAT
                     LRARVPFLHAFDGFRTSHEVQKVEQLTDDDIRALIDDDLVRAHRARALSPDHPVIRGT
                     AQNPDVFFQAREACNPFYNAVPGIVEEVMDQFYAQVGRRYHLFDYMGAPDAERVIILM
                     GSGCETAEEAVTHLVERGEKVGLIKVRLFRPFSVAHFLKA"
BASE COUNT          313 a          576 c          589 g          305 t
ORIGIN      
        1 cgccttgagg aagtgcgcca ccgagaaggg gcggaagagg cggaccttga tcagaccgac
       61 cttctcgccg cgctcgacca ggtgggtgac ggcttcctcg gccgtctcgc acccggagcc
      121 catcaggatg atcacgcgct cggcatcggg cgcgcccatg taatcaaaga ggtggtagcg
      181 gcgtccgacc tgcgcgtaga actggtccat cacctcttcg acgatacctg ggacggcgtt
      241 gtagaagggg ttgcacgcct cgcgcgcctg gaagaagacg tccgggttct gcgcggtgcc
      301 gcggatcacc ggatggtcgg gcgagagagc gcgggcgcgg tgagcgcgca ccagatcgtc
      361 atcaatcagc gcccggatgt cgtcgtcggt gagctgctcg accttctgaa cctcgtgcga
      421 ggtgcggaag ccgtcaaacg cgtggaggaa ggggacgcgc gcgcgcaacg tggctgcctg
      481 cgcgatcaga gcaaagtcca tcacttcctg caccgagttg gagaagagca tcgcccaacc
      541 ggtggcgcgg cacgccatca cgtcgctgtg atcgccaaag atagagagcg cgtgcgtggc
      601 cagggtgcgc gcggtgatat ggaacaccgt cggcgtcagc tctccggcga tcttgtacat
      661 gttggggatc atcaagagga ggccctggct ggccgtaaag gtggtggtca acgcgcccgc
      721 ctgcagcgag ccatgcacgg cccctgccgc gccgccctcg gactgcatct cctgcaccag
      781 cggaacctgg ccccaaatgt tggtctgccc agccgccgcc cacgcgtcgc ccaactctcc
      841 cattggcgac gagggcgtga tcgggtagat cgtcatcacc tcgttcgttt tgtaggctac
      901 gtaggcggct gcttcattgc cgtctatcgt tacccgttta cgtgccattt tgcggtcgat
      961 cctttctgag atcctgaaca cccaatgcgc cgcgcctctt ctcgaaagca tccagagccg
     1021 caggattcac gcctgcgcgc caaaacggcg ttcctgctgt gaatgcttca cgagccgcgc
     1081 gcgcggccgg ccctatccgt gcgcccctgg tggcgcccag aggcgcgcaa cgcgcttgtg
     1141 acttcgagat ccccctgcac gatgcggaag cgcaagcacg caagggccct tcagggttgc
     1201 cgcgcgcagc ccatgccagg gcatggagac cagcccggca ggcccggccc cacgccagat
     1261 cggccgcgca ggccgctcgc ggctcggcct ggccggccgg ctgccccgtc gcgacgcaat
     1321 gagcggcgga tttcggggcg tctctctgtc cgatggcggt cctggggaga taccacgccc
     1381 gaaggaggat accatagtta ccgcaggaat ggtatcggtg aaacccccca tcacaatcgg
     1441 gcaattctct taccttctgc tcccctcccc cgcggcgcgc accgctcgcg acagcggtgc
     1501 gaatcgggac aaccagcgcg cggcggctgc cctgttctat ccaggagata gtgtacacca
     1561 tccagggtcc caggtcaacc cggagaccgg cgcttggtcc gcgcgcggct gtgagctggc
     1621 aaggcagcaa agatttgttc gtggcgaaaa agagggctga ttcccgcctt gtaatcaggg
     1681 gagcgctttt cgggcactcg atggagagga acccggacgc gcgtgccgtg cgaagggggg
     1741 agggtgatgc ctgatgctgg aggagtgcct gaacgacttt gac
//
LOCUS       DAOSCV010000948         1782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44294_length_1782_cov_2.624204, whole genome shotgun sequence.
ACCESSION   DAOSCV010000948 DAOSCV010000000
VERSION     DAOSCV010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44294_length_1782_cov_2.624204"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1782
                     /locus_tag="PLY56_17980"
     CDS             <1..>1782
                     /locus_tag="PLY56_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOJ23417.1"
                     /translation="YAQAWAAARHGTTNMSTLIAFGTSAAFFYSLVALLFPTAIAPEG
                     HAPLYFDTSAAIIALILLGRTLEARAKGQASQAIRRLVGLRPRTARVVRDGTEVDLPI
                     DDVQVGDVVVVRPGEKIPVDGEVLEGASAVDESMVTGESIPVEKHPGDLVIGATLNRA
                     GTFRFRATRVGKETMLAQIIRLVEEAQARKAPIQHLADVVASYFVPAVIAIAVLTFLG
                     WYAFGSAYVPAGQTRFTFALLLFVAVVIIACPCSLGLATPTAILVGTGKGAELGILIR
                     GGEALQIAERLDAIILDKTGTLTRGEPWVTDIIPAGGVDSTELLRLAASVERGSEHPL
                     GEAVVREAQAQGLALAPAQEFEALPGRGVRARVDERALWLGSPRLLAERGLELGPLGE
                     AVRRLTEAGKTPLCVADEDRVLGVLAVADTLKPGAAEAVARLRQMGLEVVLLTGDNRR
                     TAEAVAREAGIDRVLAEVLPGEKAEEVRRLQAVGKRVAMVGDGINDAPALAAADIGIA
                     IGTGTDVAIESSDITLVRGDVRSIGTAIALSRATMRTIRQNLFWAFFYNAALIPLAAG
                     VLYPAFGILLNPMLAAAAMAFSSVSVVL"
BASE COUNT          260 a          610 c          623 g          289 t
ORIGIN      
        1 tacgcgcagg cgtgggccgc agccaggcac ggcaccacca acatgagcac gctgatcgcg
       61 tttggcacca gcgccgcttt cttctatagc ctggtggctc tgctctttcc caccgccatc
      121 gcgccggagg gtcacgcccc gctctacttc gacacctcgg cggccatcat cgccctcatc
      181 cttctcggtc ggacgctgga agcccgcgcc aagggccagg cgtctcaggc gatccgccgg
      241 ctcgtcggcc tgcgtccgcg aacggcacgc gtggtgcgcg acggaacgga agtcgatctc
      301 cccatcgacg acgtgcaagt cggcgacgtg gtcgtggtcc ggcccggcga gaagatcccc
      361 gtggatggcg aggtgctcga aggcgcctcc gcggtggacg agtcgatggt gaccggcgaa
      421 agcatccccg tggagaagca tcccggtgac ctggtgatcg gggccacgct caaccgggcg
      481 ggcactttcc gcttccgtgc cacgcgcgtc gggaaggaga cgatgctcgc ccagatcatc
      541 cgcctggttg aggaagcgca ggcgcgcaaa gcgccgatcc agcacctggc cgatgtggtg
      601 gcgagttact tcgttccagc ggtgatcgcg atcgccgtgc tcaccttcct gggatggtat
      661 gccttcggga gcgcctacgt accggcgggg cagactcgct tcaccttcgc gctgctcctc
      721 ttcgtggcgg tggtgatcat cgcctgcccc tgctctctcg gattggcgac gcccaccgcg
      781 atcctcgtcg gcacgggcaa aggcgccgag cttggcatcc tgatccgggg gggcgaggcg
      841 ctccagattg cagagcgcct ggatgccatc atcctcgaca agaccggcac gctgacccgg
      901 ggcgagccgt gggtcaccga catcatcccc gccgggggcg tagattcgac ggagctgctc
      961 cgcctggccg cctccgtaga acgcggatcg gagcaccccc tcggggaggc cgtggtgcgc
     1021 gaggcgcagg cccaggggtt ggcgctcgct cccgcccagg agttcgaggc gctgcccggg
     1081 cggggggtgc gcgcccgcgt ggatgaacgc gcactctggc tgggaagccc ccgccttctt
     1141 gcggagcgtg ggctggagct gggccccctc ggggaagccg tccggcgcct cactgaagcg
     1201 gggaagacac ccctctgcgt ggcagacgaa gaccgggttc tgggcgtcct tgcggtggcc
     1261 gacacgctga agccaggagc ggcagaggcg gtggcgcgcc tgcgccagat gggcctcgag
     1321 gtggtcctgc tcaccggcga caaccggcgc acagccgaag cggtcgcgcg cgaggcgggg
     1381 atcgaccgcg tgcttgccga ggtgcttccg ggggaaaagg ccgaggaggt gcgccggctt
     1441 caggcagtgg gaaagcgcgt ggcgatggtt ggtgatggga tcaacgacgc cccggcgctc
     1501 gccgcggccg atatcgggat cgccatcggc acgggcacgg acgtggctat cgagtcgtca
     1561 gatatcaccc tggtgcgagg cgacgtgcgc tccatcggca ccgccatcgc gctcagccgg
     1621 gcgacgatgc gcaccatccg ccagaacctt ttctgggcgt tcttctacaa cgcggcgctc
     1681 atccctctgg ccgccggcgt cctctacccg gcgttcggca tccttctcaa cccgatgctc
     1741 gccgccgcgg caatggcgtt ctcttcagtc agcgtcgtgc tc
//
LOCUS       DAOSCV010000949         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44522_length_1776_cov_3.169669, whole genome shotgun sequence.
ACCESSION   DAOSCV010000949 DAOSCV010000000
VERSION     DAOSCV010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44522_length_1776_cov_3.169669"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1776
                     /locus_tag="PLY56_17985"
     CDS             <1..>1776
                     /locus_tag="PLY56_17985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23418.1"
                     /translation="WRFNRIPLKSGEALETDVTLIPFQGLERVDGVAQDVIGAISLED
                     EESAVVTLCLASPESKPATGLIRLRRLPDGPWQELGRLDVSPGKPVTKRVDLARLATG
                     GYLLNCQVRRGGQDLDDFERAFARGGARLAYTRAPREERVGLAAEEPPALPRHDLTDS
                     IVTPHVAWARPLPGGPIKAVVLCDDTMAREVIELKQRLDLDPTYVKFRTHHWKEELWC
                     GDRSISTPEQANRRLLEYLKANRYELFVLAGFDWHAHFTPEVRDAITAQVRDGAGILF
                     IMPDGFKADDPLAPMMGLAKGRMMHAWAEWKREGDSPLTAGLPWRLFPQTRFMTYERP
                     PEGNVLARLSNGQPLLVTNQLGKGRTVALTYDVLTHEMSYRGYAGLTPTISYRGGFLR
                     PEYAEMTWAYHEPWYALLTRLCAWAAGRDTGVRLASLEPVEDAASAKALSLKLEGGKE
                     TYRVSAQFRNRWSHPLQQVEVSCRPGESEVNIPIPDRLGAGVNLVDVIVRDAAGRSVA
                     WGQTYIRAKAPLAIKSVLPEKEILFGERAPRGDQPYDRAFRPREPFRVKVTLESAGTP
                     PAGSRLRARLYDTHRRLLFEETRALP"
BASE COUNT          283 a          595 c          617 g          281 t
ORIGIN      
        1 gtggcgcttc aaccggatcc cgctcaagag tggtgaagcg cttgagacgg atgtgacgct
       61 catccccttc cagggcctcg agcgcgtgga tggcgtggcg caggatgtga tcggcgcgat
      121 ctcgctggag gacgaagaga gtgccgtcgt gacgctgtgc ctcgcctcgc ccgagtcgaa
      181 accggcgacg gggctgattc gcctccgccg gctgccggac gggccgtggc aggagctcgg
      241 ccggctggat gtctcgcctg ggaagccagt cacgaagcgt gtcgacctgg cccggctggc
      301 cacaggcggt tatctcctta actgccaggt gcggcgcggc ggtcaggatc tggacgattt
      361 cgagcgcgcc tttgcccgag gtggcgcgcg cctggcctac acgcgagcgc cgcgagaaga
      421 gcgggtgggc cttgccgcag aggagccccc cgccctgccg cggcatgatc tcactgattc
      481 catcgtcacg ccgcacgtcg cctgggcacg cccgctcccc ggcgggccga tcaaggcggt
      541 ggtcctctgc gacgacacga tggcacgcga ggtgatcgag ctgaagcagc gcctcgatct
      601 cgacccgacc tacgtgaagt tccgcaccca tcactggaag gaagagctgt ggtgtggcga
      661 ccgctccatc tcgacgccgg agcaggcaaa ccggcgcctc ctggaatacc tgaaggccaa
      721 ccgctacgag ctgttcgtgc tcgccggatt cgactggcac gcccatttca cgcccgaagt
      781 gcgcgacgcg atcaccgcgc aggtgcgtga tggcgccggc atcctcttca tcatgccgga
      841 tggattcaag gcggatgacc cgcttgcccc gatgatgggg ctcgcgaagg gccggatgat
      901 gcacgcctgg gcggagtgga agcgtgaggg cgactcgccc ctcactgccg gcctcccctg
      961 gaggcttttc ccgcagacgc gctttatgac ctacgagcgc ccgcccgagg gcaacgtgct
     1021 tgcgcgcctg agcaacggcc agccgctcct cgtcaccaac cagcttggga aggggcgcac
     1081 ggtcgcgctc acctacgacg tgctcaccca cgagatgtcg taccggggct acgcggggct
     1141 gacgcccacc atctcgtatc gcggcggctt cctccggccc gagtacgcgg agatgacctg
     1201 ggcctatcac gagccgtggt atgctctcct gacgcgtctc tgcgcctggg cggccgggcg
     1261 cgacacggga gttcgcctgg catcgctcga gccggtggag gacgcagcta gcgcaaaggc
     1321 actttcgctg aaactggagg gcggcaagga gacgtatcgg gtgagcgccc agttccgcaa
     1381 tcgctggtcg cacccgctcc agcaggtgga ggtcagctgc cgtccgggag agtcggaggt
     1441 aaacatcccg atcccggacc gcctgggcgc cggggtgaat ctggtggatg tgatcgtccg
     1501 cgacgccgcc gggcggagcg tcgcgtgggg ccagacttac atccgggcga aggcgccgct
     1561 cgctatcaag agcgtgcttc cggagaagga gatcctcttt ggtgagcgcg cgccaagagg
     1621 ggatcagccg tatgaccgcg cgttccgccc gcgagagccg ttccgcgtca aggtgacgct
     1681 tgagagcgcg ggcacgccac cggcgggatc ccgcctgcgg gcgcgcctct acgacacgca
     1741 ccgccgcctc ctcttcgagg agacgcgcgc gctgcc
//
LOCUS       DAOSCV010000950         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44630_length_1773_cov_3.916182, whole genome shotgun sequence.
ACCESSION   DAOSCV010000950 DAOSCV010000000
VERSION     DAOSCV010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44630_length_1773_cov_3.916182"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLY56_17990"
     CDS             complement(<1..424)
                     /locus_tag="PLY56_17990"
                     /inference="COORDINATES: protein motif:HMM:NF015425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HOJ23419.1"
                     /translation="MSKELSPLEVAKQSVDPYAGEAVFARLSRATVEDITPEEDIVMR
                     WHGLYRQRPAEAGLFTLRVRLPNGVLTTEQATVLAGIAARMCGGRLDVTTRQSIEVHG
                     LPLAALEGVFHELRAAGLTTLGACGDTVRGMVACPAAGL"
     gene            660..1622
                     /locus_tag="PLY56_17995"
     CDS             660..1622
                     /locus_tag="PLY56_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOJ23420.1"
                     /translation="MTRALFVRQGHGPLLTPDDLPFHANAVLNPGLAIVGDETVLLLR
                     IEDRQGLSQIRVARSYNGIDGWRIADRPLLEPDLPDFPYEEWGCEDARVTQIGESLWI
                     IAYTAYSRYGPAVALATTEDFETVERLGIVLSPSNKDATLFPYPFDGEWVMLHRPVTG
                     GQEHIWHATADHDFYHWSRPGVLLPERGGPWWDGLRIGGGAPPILTDEGWLLIYHGVK
                     EMAGHPIYRLGVALLDRDNPRRVLARASEWVFAPETDYEQRGLVPNVVYTCGAVRRGD
                     EVWMYYGAADTVVGLAIAQVSDLLTFVREKDYLSRIGLEKGMEP"
BASE COUNT          294 a          615 c          582 g          282 t
ORIGIN      
        1 cgaggccggc cgcggggcag gcgaccatcc cacgtaccgt gtctccacag gcgcccaagg
       61 tggtgagccc ggccgcccga agctcgtgga agacgccctc cagggccgcg agcggcagcc
      121 cgtgcacctc gatgctctga cgggtggtca cgtcgaggcg cccgccacac atgcgcgcgg
      181 cgatacccgc gagcaccgtg gcctgttccg tggtgaggac gccgttgggc aggcgcacgc
      241 gcagcgtgaa gagcccggct tcggccgggc gctgccggta gagaccgtgc cagcgcatca
      301 cgatatcctc ttccggcgtg atatcttcca ccgtcgcccg cgataaacgg gcaaagaccg
      361 cctcgccggc gtatggatcc accgattgct tggccacctc gagcggggaa agctccttcg
      421 acatcgaaac gacacccttc cttgccggcc agaccaccgg cctcatcatg agcgaacact
      481 gcgtccctct gatctccgat cttcgccacg ccaggagcga tctcccaccc ggctcgcgca
      541 aggagccgga tgccaggttt ggcactcggg ggcgtagggg taatctgcaa gaaacgcccg
      601 tgtggtgggc gcccgtcgag gccgttcgcg agatcctcgc cggggaggag agattcggga
      661 tgacacgtgc gctctttgta cgccagggcc atggcccgtt gctcacacca gacgatctcc
      721 ccttccacgc aaatgcggtc ctgaacccgg gtctcgccat tgtgggcgac gagacggtcc
      781 tcctcctgcg cattgaagac cggcaggggc tctcgcagat ccgcgtggcg cgcagctaca
      841 acgggatcga cggatggcgc atcgccgacc ggcccctcct tgagccggac ctacccgatt
      901 tcccctacga ggagtggggc tgtgaggatg cgcgcgtgac gcagatcggg gagagtcttt
      961 ggatcatcgc ctatacggcc tactcgcgct atggcccggc agtcgccctc gccaccacgg
     1021 aagacttcga gacggtcgag cgcctgggga tcgtcctttc gcccagcaac aaggatgcca
     1081 cgctcttccc atacccgttt gacggcgagt gggtgatgct ccaccggcca gtgaccggcg
     1141 gccaggagca tatctggcac gccacggcgg accacgattt ctaccattgg agccggcccg
     1201 gcgtgctgct gccggaacgg ggaggcccct ggtgggatgg cctgcgcatc ggtggcggcg
     1261 cacccccgat cctcaccgac gagggctggc tcctcatcta ccacggggtc aaggagatgg
     1321 ccggccaccc gatctaccgc ctgggcgttg ccctgctcga ccgcgacaac ccgcgccgcg
     1381 tgctcgcccg cgcctccgag tgggtcttcg ccccggagac agactacgag cagcgcggcc
     1441 tggtgcccaa cgtcgtctat acctgcggcg ccgtccgtcg cggcgacgag gtgtggatgt
     1501 actacggcgc cgccgatacg gtggtcggcc tggcgatcgc ccaggtctcc gacctgctca
     1561 ccttcgtgcg cgagaaggac tacctgagcc gcattggcct ggagaaaggc atggagccct
     1621 gaggattctc ccgcacggcg tgccctccgg gtgagattcc gagataggga atccgaagcc
     1681 tcggaggcag cgcgcacccc ggtgcgcggc gctaaaagca ccgcgctgct tgcaagcgaa
     1741 gccctgccgg gctggattgg ggatactccc aga
//
LOCUS       DAOSCV010000951         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44634_length_1773_cov_3.087311, whole genome shotgun sequence.
ACCESSION   DAOSCV010000951 DAOSCV010000000
VERSION     DAOSCV010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44634_length_1773_cov_3.087311"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="PLY56_18000"
     CDS             complement(<1..1767)
                     /locus_tag="PLY56_18000"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOJ23421.1"
                     /translation="MAASADLGTEKRQQSERKRRAENLHKVFLSMARDLRVVVIKMAD
                     RLHNMRTLEHLPPERRIRIARETLQIFAPLAHRLGIWHLKWELEDLSFKYTEPEKYEE
                     VARLVAKTRAEREGELAAAIEILRERLRASGIEAEIQGRPKHLYSIYQKMLSQELDFS
                     DIYDLMAVRIIVNTLEECYHALGVVHDLWVPLRGFFADYVAKPKPNNYQSLHTKVIGP
                     SGEPMEIQIRTWAMHRTADFGVAAHWRYKEGTKEDSFERRLAMLRQQLIASQSDTRDP
                     QDFLRTVMENILADQVFVFTPKGEVIDLPAGSTPVDFAYRIHSDLGDHCIGAKVNGRM
                     VPIDYRFSNGDVVQIITRNTSGPSLDWLKFVKTSTARNRIKRYLRRLTRDEDIARGRE
                     QLERELERRNLERIDLSRHERAAEVAAEMNYQSVDDLLAAVGHGLVSVHKVVERLTLQ
                     PVLETPVISVPVPPRTEQPAGKIGITVGGQEGLLLKLGKCCSPLPGDAVTAYISRGRG
                     LVIHRVDCPNAIKYYQKEPERLVAVDWQDQDDAVYPAHLQVLVVDRVGALNAVVSIIS
                     ESRVNITRMKVSTAPNRTGLLDL"
BASE COUNT          291 a          582 c          562 g          338 t
ORIGIN      
        1 gaggtcgagc aggcctgtgc ggttgggcgc ggtgctcacc ttcatccgcg tgatgttcac
       61 ccggctctcg ctgatgatcg agaccaccgc attgagcgcg ccgacacggt ccaccaccaa
      121 cacctgcagg tgtgccggat agacggcatc gtcctgatcc tgccagtcga cggcgacgag
      181 gcgctctggc tccttctggt agtatttgat ggcattgggg caatccacgc gatggatgac
      241 gaggccacgc ccgcgcgaga tgtaggcggt aacggcatct cccggaagtg ggctgcagca
      301 cttgcccagt ttcaggagca ggccctcctg gccaccgacg gtgatcccaa tcttgcctgc
      361 gggttgctcg gtgcgcggcg gcactggcac agagatgacg ggcgtctcca gcaccggctg
      421 cagcgtgagg cgctccacca ccttgtgcac gctcacgagg ccgtggccca cggcggcaag
      481 caggtcatcc accgactgat agttcatctc ggcggcaacc tcagccgcgc gctcgtgccg
      541 cgagaggtcg atccgctcca gattccttcg ctccaactcg cgctcgagct gctcgcgccc
      601 gcgcgcgata tcctcatcgc gcgtcaggcg gcgcagatac cgcttgatgc ggttgcgcgc
      661 cgtggaggtc ttgacaaact tgagccagtc caggctgggg ccgctggtgt ttcgcgtgat
      721 gatctgcacc acgtcgccgt tgctgaaccg gtagtcgatc ggcaccatgc gcccgttgac
      781 cttggcgccg atacagtggt cacccagatc actatggatg cggtaggcga agtcgaccgg
      841 cgtcgaaccg gccggcaggt cgatgacctc ccccttcggt gtgaagacaa agacctgatc
      901 ggcgaggata ttctccatca ccgtgcggag gaagtcctgc ggatcgcgtg tgtcgctctg
      961 cgaagcgatg agctgctggc ggagcatggc gagccggcgc tcgaaggagt cttccttggt
     1021 cccctccttg tagcgccagt gcgcggccac gccgaagtcc gcggtacggt gcatcgccca
     1081 ggtgcggatc tggatctcca tcggctcgcc cgagggcccg atcaccttcg tatggagcga
     1141 ctggtagttg ttcggctttg gcttcgccac atagtccgcg aagaagccgc gcagcggcac
     1201 ccacaggtcg tggaccaccc ccagggcgtg gtagcactcc tccagggtgt tgacgatgat
     1261 gcgcaccgcc atcagatcgt agatatcgga gaaatccagc tcctggctga gcatcttctg
     1321 atagatgctg tagaggtgct tgggccggcc ctggatctcc gcctcgatgc cggaggcccg
     1381 caggcgttca cgaaggatct cgatcgcagc cgccagctcg ccttcgcgct cagcgcgagt
     1441 cttggcgacc agcctggcca cctcctcgta cttctccggc tcggtgtact tgaaggagag
     1501 gtcctccaac tcccacttca ggtgccagat cccgaggcga tgggccaatg gcgcgaagat
     1561 ctgcagcgtc tcgcgcgcga tgcggatgcg cctctccggc ggcaggtgct ccagggtgcg
     1621 catgttgtgc agccggtcgg ccatcttgat gacgaccacc cgcagatcac gcgccatgga
     1681 gaggaaaacc ttatgcaggt tctccgcccg ccgcttccgc tccgactgtt gccgcttctc
     1741 ggtgcccagg tccgctgaag ccgccatctc gtc
//
LOCUS       DAOSCV010000952         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44638_length_1773_cov_2.930151, whole genome shotgun sequence.
ACCESSION   DAOSCV010000952 DAOSCV010000000
VERSION     DAOSCV010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44638_length_1773_cov_2.930151"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLY56_18005"
     CDS             <1..512
                     /locus_tag="PLY56_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23422.1"
                     /translation="TRGWSAREPAEDQGGPGRGAFPFATSSPGPHTPEVIMVTGRTYD
                     CLAVANAALCASGTVTLESAILGVPHLIVYRGSFGMRLQYQLVKHKIGYIGLPNLLLD
                     ASICPELLDTAATPERIADECTALLLDEERARAQKDGFARLMELLGQPGVIRRAAERI
                     LDVARRTQA"
     gene            487..1050
                     /locus_tag="PLY56_18010"
     CDS             487..1050
                     /locus_tag="PLY56_18010"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOJ23423.1"
                     /translation="MSREERKPERLLNAVARRRTLLLVILPLIGMLVAEPRPALYLVG
                     VLAVVAGQAVRFWAAGYIVKATQLTTTGPYAHVRNPLYLGSLLITLGWGTMSGRLVVA
                     VLLLVVFALTHALAIRSEESYLGERFGEEFHAYCRQVPRLFPRLRPGTSGSGDYSWRR
                     ALQCDGEGWTSLGVAALAGLYATRFWW"
     gene            1066..>1773
                     /locus_tag="PLY56_18015"
     CDS             1066..>1773
                     /locus_tag="PLY56_18015"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOJ23424.1"
                     /translation="MRSATGAGWGAHGAKPCKKGSGFQRMLGRLRLLVLPESHDSVWR
                     LWEYTRVDLRRIVFGCLCGIGYVLLTLATAYLIRAMLGALVDANPRKIALCAAATPLL
                     YLIKGCFYYGQVYLLSSAAERLAQRLRNRLYEHMQTLSLSFFENQKTGGLMATIASDV
                     PVIQQTFTVAILEGVIGPAMVVGGLLVMFYMNWRMAIVTIVSLVAIGSVISKASLRMR
                     RASEAIQKSIGATSGLAT"
BASE COUNT          273 a          537 c          625 g          338 t
ORIGIN      
        1 gtacgcgggg ctggagcgcg cgagagcccg cggaggacca gggcggtccg gggcgcgggg
       61 cgtttccgtt cgcgacctct tcgcccggac cgcacacgcc ggaggtgatc atggtcaccg
      121 ggcgcaccta tgactgcctg gccgtggcga acgcggcgct gtgtgcctct ggcacggtga
      181 cgctggaatc ggcgatcctg ggagtgcccc acttgatcgt ttaccgcggc tcgttcggga
      241 tgcgcctgca gtatcagctt gtgaagcaca agatcgggta tatcgggttg cccaatctgc
      301 tcctggatgc gtccatctgc cccgagcttc tggatacagc agcgacaccg gaacgcatcg
      361 cggacgagtg caccgcactg cttctcgatg aggagcgcgc gcgcgcgcaa aaggatggct
      421 ttgcccgcct gatggagctg cttggacaac cgggcgtgat tcgccgggcc gcggagagga
      481 tcctggatgt cgcgagaaga acgcaagcct gagcgactgc tgaacgcggt ggcgcgccga
      541 cgcacgctcc tgctcgttat tctccccctg attgggatgc tggtggccga gccgcgcccg
      601 gctctctatt tggtgggggt gctagctgtg gtggccggac aggccgtgcg cttctgggca
      661 gccgggtaca ttgtgaaggc aacgcaactc acgacgacag gtccctacgc gcatgtaagg
      721 aaccctttgt atctgggaag cctcctgatc acgctgggct ggggaacgat gtcggggcgc
      781 ctggtggtgg cggtgctcct gctcgtggtg ttcgcgttga cccacgcgct ggcgatccgc
      841 tcggaagaga gctacctggg cgagcgtttc ggcgaggagt tccacgccta ctgccggcag
      901 gtgccgcggc tttttccccg gttgcgcccg gggacgagcg gctcaggaga ctactcctgg
      961 cggcgcgccc tccaatgtga tggggagggg tggacctccc tcggagtggc tgcgctggcg
     1021 gggctctatg cgacacggtt ctggtggtga tggaggttcg gggagatgag gtctgcgacc
     1081 ggcgccggat ggggcgcgca cggtgcgaag ccctgcaaga aggggagcgg gttccagcgc
     1141 atgttgggcc ggctgcgcct tctcgtgctc cccgagtcgc acgactccgt ctggcgcctg
     1201 tgggagtata cacgcgtcga cctgcgccgg atcgttttcg ggtgcctgtg cggcatcgga
     1261 tacgttcttc tgacgctcgc gaccgcctat ctgatcaggg cgatgctagg ggcattggtg
     1321 gatgcgaacc cgcgcaagat cgcgctctgc gccgccgcga ccccgctcct ctacctgatc
     1381 aagggatgct tttactacgg gcaggtttac ctcctctcga gtgccgccga acgcctggcg
     1441 cagcgcctcc gcaaccgcct ctacgagcac atgcagacgc tctccctctc cttctttgag
     1501 aaccagaaga ccgggggact gatggccacg atcgccagcg atgtgccggt gatccagcag
     1561 acgttcacgg tggctatcct cgaaggggtg atcggcccgg cgatggtcgt cggcggcctc
     1621 ctcgtgatgt tctacatgaa ctggcggatg gcgatcgtga cgatcgtctc gctggtcgct
     1681 atcgggagcg tcatctccaa ggcaagcctg cggatgcgcc gggcgagcga ggcgatccag
     1741 aagagcattg gagcgacatc agggttggcg aca
//
LOCUS       DAOSCV010000953         1772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44670_length_1772_cov_4.964473, whole genome shotgun sequence.
ACCESSION   DAOSCV010000953 DAOSCV010000000
VERSION     DAOSCV010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44670_length_1772_cov_4.964473"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLY56_18020"
     CDS             <1..342
                     /locus_tag="PLY56_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23425.1"
                     /translation="RDIEPEMGEHHFRMLRAHYHAMDHTVTASEMARAIRYASYSSAN
                     LHYGKLGRKLAEALGFRLKYWVNSLADFIGPDEGLPHWLWIMWPQVGAALEELGWIST
                     DQDESGLEESP"
     assembly_gap    613..712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1009..>1772
                     /locus_tag="PLY56_18025"
     CDS             1009..>1772
                     /locus_tag="PLY56_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23426.1"
                     /translation="MRDVNAASRAKRVRGHRSVALLGMRELLAACATVLCAVLFLPHG
                     VAAQEPARPRIAFVDLALPDEGKPRESGALAFARRQGEVIRVKPLATGGWQDDSGRLR
                     APEEADLVWYHSGEVDAAARFGRAGAADLLAYLEAGGSVLLTGTAGSLLNAMGIETAP
                     LRVSTQKARQGSLRVVEKHRAHPVFAGLPTSTPIVLPTRPGSAVAGLDVPSGSAGELL
                     ADAMPGSGEGLIVEYTAGAGRVIFVGGAVPDFTAAPA"
BASE COUNT          247 a          560 c          593 g          272 t
ORIGIN      
        1 cgtgatatcg agccggagat gggcgagcac cacttccgaa tgctgcgcgc gcactaccac
       61 gccatggacc acaccgtgac cgccagcgag atggcccgcg cgattcggta tgcgtcctac
      121 tcctcagcga atttgcacta cgggaaactg gggcgtaaac tcgccgaagc cctcgggttc
      181 cggctgaagt attgggtcaa ctccctcgcc gacttcattg ggccggacga gggcctccct
      241 cactggctct ggatcatgtg gccccaggtg ggcgctgccc tcgaagagct ggggtggatc
      301 tctactgatc aggacgagag cggcctggag gagagcccct gacccgctta agccgcttcc
      361 tgccgcaagc ctgcatcttg gcaccggctc actcctgccg gcgtgactct gtgcctggcg
      421 gcagggtatc gctccgggcg cggcgaatca cgcttctggt agaagccccc atctcctatt
      481 cacgggggcg actctctggg taggactgag attccgagga taaggatccg aagcctcggt
      541 ggtgacgctc aacccggcgc gcggcgctaa agcaccgcgc tgcttgcgag cgaagccctg
      601 ccgggctggg ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccccgaa
      721 ggggctttgc tgagaggcag cgcggagctt ccagctccgc gcgcgggtgt gcagaagccc
      781 tacgcgtgcc gcgcggcgct aaagcaccgc gctgcttgcg agcgaagccc tgccgggctg
      841 ggccagccta cacgcgcgcc taaccggata tggcggggac tgggaggggg agccccgaag
      901 gggctttgct gagaggcagc gcggagcttc cagctccgcg cgcgggagct caccatccgt
      961 gtccgtcctt ccttgtctcg gaatcccaag ggtaggagca gtatcgagat gcgagacgtc
     1021 aacgccgcca gccgggcgaa gcgggttcgg ggccacagga gcgtggcgct tcttggaatg
     1081 cgggagcttc tcgcggcgtg cgccaccgtg ctctgtgcgg ttctcttctt gccccatggc
     1141 gtggcggccc aggagccggc gcgcccgcgg atcgccttcg tcgatctcgc gctgccggac
     1201 gagggcaaac cgcgcgagag cggcgcgctg gcgtttgcgc ggcgccaggg cgaggtgatc
     1261 cgggtgaagc cgctcgcaac cggcgggtgg caggacgatt cgggccggct gcgcgctccc
     1321 gaggaggcgg atctcgtttg gtatcacagc ggcgaggtgg atgccgccgc gcggtttggc
     1381 cgggccggcg ccgcggacct cctcgcctac ctggaagccg gtgggagcgt gcttctcacc
     1441 ggcactgccg ggtctcttct caacgcgatg gggatcgaaa cggcgcccct gcgcgtctcc
     1501 actcagaaag cccgtcaggg cagcctgcgc gtggtcgaga aacaccgcgc gcaccccgtt
     1561 ttcgcgggcc tgcctaccag cacgcccatc gtgctcccca cccggcccgg gagcgccgtg
     1621 gccggtctcg acgtgccatc cggatcggcg ggcgagctcc tggcggatgc catgccgggg
     1681 agcggcgagg gtctgatcgt ggagtacacc gccggcgcgg gccgcgtgat cttcgtcgga
     1741 ggggccgtgc ctgatttcac ggcagccccc gc
//
LOCUS       DAOSCV010000954         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44979_length_1763_cov_2.748244, whole genome shotgun sequence.
ACCESSION   DAOSCV010000954 DAOSCV010000000
VERSION     DAOSCV010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44979_length_1763_cov_2.748244"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(463..1095)
                     /gene="coaE"
                     /locus_tag="PLY56_18030"
     CDS             complement(463..1095)
                     /gene="coaE"
                     /locus_tag="PLY56_18030"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518962.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ23427.1"
                     /translation="MRVVGITGGIATGKSTVAAMFKECGAALIDADALSRAAVEPCQP
                     AYHAVVAQFGTGILRDDGTLDRRALGALVFRDAEARRRLEAIIHPYVVQAIQRQLAEW
                     RASPSPPPLVVVEIPLLYEAGLEEMVDTVVVVSSEQATQLQRLITRTGLAPEEAMLRI
                     AAQMPLSEKEARAAHVISTDKPLQAVREEVRRLYEDLLAGEGSELDGPTS"
     gene            complement(1179..>1763)
                     /locus_tag="PLY56_18035"
     CDS             complement(1179..>1763)
                     /locus_tag="PLY56_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ23428.1"
                     /translation="PEEGKISLGLRQILPDPWADVDKRYHVGDVCTGKVSRLVPFGAF
                     VQLDNGIEGIIPNAELAHRRVNKPEEVLSVGDEVTVKVINVRAEERRITLSLRQMQLD
                     EERREFREYQRGRGGASDRTTIGDVVGDLAAKMRRSRRERRERERVDLEDYAEEFEEL
                     EEIDADELAEGPEEAEISDEGPTAEQSESEQPQA"
BASE COUNT          291 a          597 c          563 g          312 t
ORIGIN      
        1 cgagagtgag ggaaagcaaa ctaccgaaga gtgccagaaa cagcctggtc cgcccagcag
       61 atctctcttt caacgcaggg acaccctcct actcggctac ccgctggcgt ctgtggcgat
      121 agcgtcggaa ggacacgaag gttcggttgg cagccgtgga cgtgctctga aggggcgcaa
      181 tcacgccctc gcagattata gcacaatgcc cgtgcgattg caactccaac cggctgctcc
      241 taccccccgt cgagccgaga ttcttgccgc cactcccgag ggaagccaac gcgcttgccc
      301 acacccgcgg atgccgaagt gccggcccgg tgtaggacag gcttatgtgc aatttcgcgg
      361 catcccgggg aagttcctct aatgccgttt tcactgcgcg ccggtcccgt gcgccgggag
      421 cgcgcgcaaa ccaggaacgg agccttgctg ccatgcgtcg gcctacgagg tggggccgtc
      481 gagctcgctc ccctccccgg ccaggagatc ctcgtagagc ctgcggacct cttcgcgcac
      541 ggcctggagg ggtttgtcgg tgctgatcac atgcgcggcg cgcgcctctt tctcagataa
      601 gggcatctgc gcggcaattc ggagcatcgc ctcttcaggc gccaggcccg ttctggtgat
      661 tagacgctgg agctgagtgg cttgttccga ggagacaacg accaccgtgt cgaccatctc
      721 ttccaggccg gcttcgtaga gaagggggat ctccaccacg acaaggggcg gaggagaagg
      781 gctggcgcgc cattcggcaa gctgccgttg gatggcctgc accacgtagg gatggataat
      841 cgcttcgagg cgccggcggg cctcggcgtc acggaagacg agcgcgccga gcgcgcgccg
      901 gtccagggtg ccgtcgtcgc gaaggatgcc ggttccgaac tgggcgacca cagcatgata
      961 ggcgggctgg cagggctcga cggcagcgcg cgacagcgcg tcggcgtcga tcagagcggc
     1021 gccgcactcc ttgaacatcg ccgccacggt gctctttccc gtggcgatcc cgccggtgat
     1081 gccaacgacg cgcatcgggg gcctctcgac tggggaagga gcgtaacccg ccggccgccg
     1141 ccgttgcggg cgacagccgg caggtcactt gatgtgcact acgcctgcgg ctgctccgac
     1201 tccgattgct ccgccgtggg cccctcatca ctgatttcag cctcctcggg tccttcggca
     1261 agctcgtcgg catcgatctc ttcgagctcc tcgaactctt ctgcgtagtc ttcgagatcg
     1321 acgcgctcgc gctccctccg ctcgcggcgc gagcggcgca tcttagcggc aagatcgcct
     1381 acgacgtcgc cgatggtggt gcggtccgag gcacctccgc gcccgcgctg atactcgcgg
     1441 aactcgcggc gctcctcatc cagctgcatc tggcgcaggc tgagggtgat gcggcgctcc
     1501 tcggcgcgca cgttgatgac cttcacggtc acctcgtcgc ccacagagag cacctcttcc
     1561 ggcttgttga cacgccggtg cgccagctcc gcatttggaa tgatcccctc gatgccattg
     1621 tccagctgga caaacgcgcc gaagggcacc aggcgcgata ctttgcccgt gcatacatcg
     1681 cccacgtggt agcgcttgtc cacgtcggcc cacggatcgg ggagaatctg gcgcagaccc
     1741 agcgagatct tcccctcctc cgg
//
LOCUS       DAOSCV010000955         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_44988_length_1763_cov_2.459016, whole genome shotgun sequence.
ACCESSION   DAOSCV010000955 DAOSCV010000000
VERSION     DAOSCV010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44988_length_1763_cov_2.459016"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            236..>1763
                     /locus_tag="PLY56_18040"
     CDS             236..>1763
                     /locus_tag="PLY56_18040"
                     /inference="COORDINATES: protein motif:HMM:NF025086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOJ23429.1"
                     /translation="MKTPGVPERMFLATRSRKVAALLARRWREVRAWARALRHRMGTR
                     AGIPLAGARRWVRAGLPGPLVLSVVIHAVGLGALGRVTFRTPTASTPRYTRIRLMKWE
                     PAQQPEGVPDVSLPGGRARAVALPPTVASDPTLEARRQAEARIAALRADFFAPMRISI
                     LPMPVLPWFDDDEKLLPPPLWNVPLGGNLRPLPSESSLGVDPEAHDAPEPPARAEKPA
                     DSPAVVDTPDEPAATEQPVEVAEAPAGPVVSVPDEQPRDPVMEATRGTSTAASPEGVL
                     VAQAEPGDAPRAEETPAAETPELPKPDAATPESATPVTADPGGGMEMSGAPAREAAPA
                     GEASTTLPEAPAPVVETPSEAKASSSEERPKDGSGAAAVARTEKPQIPADGTHAGLTG
                     SFRVLQGLALPGLPPSGSMPALLAEIGKRTALKVTSAGTVAPDAIPADALLVYLRAAS
                     KVTLDPQERAALSAYLRGGGILFIDGADGEAGDALRAQMEALAGGKAERLPATHALYS
                     A"
BASE COUNT          279 a          581 c          646 g          257 t
ORIGIN      
        1 cctgccgtcc agctagagca ggactgaacg ccggatctca gcaccaggtg tcaggaagcg
       61 gggaatacct tttctcactg gatagactgg ggcgggtgac ctgtgttctg ttgtctcgga
      121 cgcgtgtgac tatggggatc ggatagcggg gatggcggcg aagaggaaag ggcggaggaa
      181 agcggaatcg cgccggcgcg ccttgaccag gcgcctccac cgggcggagc agccggtgaa
      241 gacgcctgga gtgccggagc gaatgttcct ggcgacgcga tcccggaagg tggcggcgct
      301 gcttgcacgg cgatggcggg aagtgcgcgc ttgggcgcgc gccctgcgcc accggatggg
      361 tacgcgcgcg gggataccgc tcgcgggcgc gcggcgttgg gtccgtgccg gtctgccggg
      421 cccgctcgtg ctgtcggtgg tgatccatgc cgtcgggctt ggggcactgg ggcgcgtcac
      481 gtttcggact cctaccgcca gcacgccgcg ctatacgcgg attcgcctga tgaagtggga
      541 gcctgcccag cagcccgaag gggttccgga tgtgagcctg ccgggcgggc gcgcgcgcgc
      601 cgtagcgctt ccgccaaccg tcgcgtcgga tccaaccctc gaggcacgcc ggcaggctga
      661 agctcggatc gcggcgctcc gtgccgactt cttcgcaccg atgcggatct ccatcctgcc
      721 gatgccggtg ctcccgtggt tcgacgacga cgagaagctg ctcccacccc ctctttggaa
      781 tgtgcccctc ggcggcaacc tgcgcccgct tccctcagag agctctctag gcgttgaccc
      841 ggaggctcat gacgcgccgg agccaccggc gagagctgag aagcccgcgg attcgcccgc
      901 tgttgtggat actccggatg agcccgcggc aaccgagcaa cccgtggagg tagcggaggc
      961 ccctgcaggg ccggtggtct ccgtgcccga tgaacaaccc agagatcccg tcatggaagc
     1021 gacgcgcggc acgagcacgg ctgcctcgcc ggagggcgtc ctggtggcgc aggccgagcc
     1081 gggggatgcc cctcgcgccg aggagacccc ggctgccgaa acgccggaac tgccgaagcc
     1141 ggacgcggcg acacctgaga gcgcgacacc agtgacagca gatccaggcg ggggcatgga
     1201 gatgtccggc gcgccggcca gagaagcagc gcccgcgggc gaggcctcca cgaccctgcc
     1261 ggaagcgcct gctcccgtcg tggagactcc ttctgaggca aaggcatctt ccagcgagga
     1321 gaggccgaag gacggatcag gagcggcggc ggttgccagg acagagaagc cacagatccc
     1381 cgcagatggg actcacgcgg gcctgacggg cagcttccgc gttctgcagg gtctcgcgct
     1441 ccctgggttg cctccgagcg gctcaatgcc ggcgctcctc gcggagattg gcaagcgtac
     1501 ggcgttgaag gtgacctccg ccggcacggt cgcgccggat gcgatccccg ccgatgcgct
     1561 gctggtctac ctccgagccg cgagcaaggt aacgctcgat ccgcaagagc gcgctgctct
     1621 atccgcgtac ctgcgggggg gcggcatcct ctttatcgat ggcgccgatg gagaggcggg
     1681 cgacgcgctg cgcgcccaga tggaggcgct cgcgggtggc aaagccgagc ggctgccggc
     1741 aacgcacgcg ctctattccg cgc
//
LOCUS       DAOSCV010000956         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45154_length_1758_cov_3.010570, whole genome shotgun sequence.
ACCESSION   DAOSCV010000956 DAOSCV010000000
VERSION     DAOSCV010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45154_length_1758_cov_3.010570"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLY56_18045"
     CDS             <1..216
                     /locus_tag="PLY56_18045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23430.1"
                     /translation="RALVSHPQPAPPVVSTSGTFLLTGELPEPSEEKAPERPTDPAHL
                     PGRLILEWADEIEAEDEEGRSDLPKPS"
     gene            complement(382..1392)
                     /locus_tag="PLY56_18050"
     CDS             complement(382..1392)
                     /locus_tag="PLY56_18050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ23431.1"
                     /translation="MEAPAVSILMPAYNASPYLEEALVSVQAQEREDWELVAVDDGST
                     DETPALLRKAAASDPRIRPLFLPHRGLVPALNAGLAACRAPLVARMDSDDRMHPARLS
                     RQVAFLAAHPEVDLVGCLIECFPRAQIQEGLARYEEWLNSLVSHEEILRDLFVESPFA
                     HPSVLFRKERVTQLGGYRDCGWAEDYDLWLRLHQAGARFAKVPEVLHFWRDRPERLTR
                     TAEEYSLRNFRRLKIHYLCQGILREPGPVLIWGAGRGGKAWCACLEEAGIEIAGFVDI
                     DPKKIGRTRRGRPVIAPEDLPPAGSLPVLVTVGVKGARELIRAALHARGYQELRDFVC
                     VA"
     gene            complement(1424..>1758)
                     /locus_tag="PLY56_18055"
     CDS             complement(1424..>1758)
                     /locus_tag="PLY56_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23432.1"
                     /translation="YDGSPSFILEPRRPGDAVPVLDAALLSFIRHVPTHLVALSGCET
                     EGMAGVQAVAESGGCVYTAPRESMLFTQFADGLRSRIAGTREIAPLVVCPVMPATREI
                     VPIALARS"
BASE COUNT          328 a          593 c          586 g          251 t
ORIGIN      
        1 cgcgccctgg tcagccatcc ccagccagcg cctccggtcg tgagcacgag cgggacattc
       61 ctgctgacgg gggagctccc agaaccgtcc gaggagaagg cgcctgaacg cccgacagac
      121 ccagcgcatc tacccgggcg cctcatcctg gaatgggccg atgagatcga ggcggaggac
      181 gaagagggac ggtccgacct gcccaagcca agctaggcga tcaaaacgtc ccccgggagc
      241 cggctcccgg gggacgcggc cttcctgcat ccctcctccg ctggcaacga taccacggat
      301 gaagagggcg gacgcgggtg ggaatgtgca ggcgcctcgg gctcctatag gcgcaggtgt
      361 tttgcacccc cgcgatgacg gtcaggcgac gcagacgaaa tcgcggagct cttgatagcc
      421 gcgcgcgtgg agcgcggcac ggatgagctc gcgtgcgcct ttcacgccca cggtcaccag
      481 cacaggtaag ctgccggcag gcggcaaatc ctccggagcg atcaccgggc ggccccgacg
      541 ggtgcgtcca atcttcttgg ggtcgatatc cacgaagccg gctatctcga tcccggcctc
      601 ctccaggcag gcgcaccatg ccttgccgcc ccgtccagcg ccccagatga gcaccggccc
      661 cggctcgcgc agaatgccct ggcagaggta gtggatcttt agccggcgga agttgcgcag
      721 tgagtactcc tccgccgttc gggtcaaccg ttccggacga tcgcgccaga agtggaggac
      781 ctctggcacc ttggcaaacc gggcccccgc ctggtgaagg cgaagccaca agtcgtaatc
      841 ctcggcccag ccgcagtcac ggtagccacc cagttgggtc acgcgctctt ttcggaaaag
      901 gacagatggg tgggcgaaag gcgactccac gaagaggtcg cggaggatct cctcgtggga
      961 gacgagggag ttgagccact cctcatagcg tgccagaccc tcctggatct gcgcgcgggg
     1021 gaagcactct atcaggcacc ccaccagatc cacctcggga tgcgcggcga gaaacgccac
     1081 ctggcgactc aggcgcgcag ggtgcatgcg gtcatcggag tccatgcgcg cgacgagcgg
     1141 cgcccggcag gcggccagcc cagcattgag cgccggcacg agaccccggt gcggcagaaa
     1201 gaggggccgg atgcgtgggt cggaagcggc agccttcctc agaagggctg gcgtctcatc
     1261 ggtggagccg tcatccaccg ccaccagctc ccaatcttcc ctctcctgcg cctgcacgga
     1321 gacaagcgcc tcctccagat aaggggaggc gttgtaggcc ggcatgagaa tgctgaccgc
     1381 cggggcttcc atgccggcat tgtaacagag gccacgcggc gggtcaagac cgggctagcg
     1441 cgatcggaac aatctctctc gtcgcaggca tcaccgggca caccacgagc ggcgcgattt
     1501 cgcgtgtgcc cgcgatccgg gatcgcaggc catccgcgaa ctgggtgaag agcatgctct
     1561 ctcgcggcgc cgtatagacg caaccaccgg actcggcgac cgcctgcacg cccgccattc
     1621 cctcagtctc gcatccggag agggccacca gatgcgtcgg cacgtggcgg atgaaggaga
     1681 ggagcgcggc gtcgagcacc ggcacagcat cgcccggtct cctgggctcc agtatgaagc
     1741 tcgggctgcc atcgtagg
//
LOCUS       DAOSCV010000957         1756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45238_length_1756_cov_4.385655, whole genome shotgun sequence.
ACCESSION   DAOSCV010000957 DAOSCV010000000
VERSION     DAOSCV010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45238_length_1756_cov_4.385655"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLY56_18060"
     CDS             <1..377
                     /locus_tag="PLY56_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23433.1"
                     /translation="RFFRKYPPAVVFSYHALASFTGNSFLVSRLGEADPDYRWQAEAW
                     SSVYGAAYDEGSEPGTTLSYACSAGSLSNWIYREFGAVCFDLELGRNDPRDCVEDRVS
                     RDLLATCRARHLAGIARVAGQI"
     gene            1095..>1756
                     /locus_tag="PLY56_18065"
     CDS             1095..>1756
                     /locus_tag="PLY56_18065"
                     /inference="COORDINATES: protein motif:HMM:NF037639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ23434.1"
                     /translation="MFRQRILRACFGLLLGLPVLAAAGCGSGDDADPILGPDSGQVRA
                     VVTVDGQPLPGVNAALTGDTTRTATTGSDGVALFTGLPNGNYTVTISGFDASRVTFSR
                     TSVPVTISESVGLVTVEFQGIGPSTAAITGVVTVNGQAPAQLSGQTVTITLVPPSGDP
                     VTGDLTGTTGEYAFRQLAAGQYTLRVTVGNDVRATTVSVAEGQQLQANLEFASLPAFN
                     QGP"
BASE COUNT          272 a          566 c          609 g          309 t
ORIGIN      
        1 tgcggttctt ccggaagtat cccccggcgg ttgtcttctc ttaccacgcg cttgccagct
       61 ttaccgggaa ctctttcctg gtctcgcggc tgggagaggc cgacccggat tatcgctggc
      121 aggccgaggc atggtcgagc gtttacggcg ccgcgtatga cgaggggagc gagccaggca
      181 ccaccctaag ctacgcctgc tccgcaggga gcctctccaa ctggatctac cgcgagtttg
      241 gcgctgtctg cttcgacctg gagctcggcc gcaacgatcc gcgcgactgc gtcgaggacc
      301 gtgtttcccg cgaccttctt gccacgtgcc gtgcccgcca cctggccggg atcgcgcgcg
      361 tcgccgggca gatctgagcc gcagcgcgag ggtgggacgc gcctcttcgg cctgcctgcc
      421 ggcgtgtgac cagcgcagag caggagtatc cggcgcactc catgcgtttt ctcggcgaag
      481 ttccagccat ctcctctccg cgatgccggg accagagcag tcgaggggtt ccagatgcgc
      541 cctgagatcc cgacataagg aatccgaagc ctcggaggca gggtgcaacc cgtcgcgcgg
      601 cgataaatca ccgcgctgct tgcgagcgaa gccctgccgg gctgggtcgg ggatactccc
      661 agatgtctca aaatggcgat ggctgggagg gggagccccg aaagggcttt gccggaaagc
      721 agcgcggagc ttcgagctcc gcgcgtgagg gcggtcctct ccttatgtcg gaatcccagg
      781 gatgcgccac gcggtggctc ttcgccgggg cttgccacgg agacggcgtt gcctgcgcgg
      841 cgcattgctg ttttgcacgc cggggcatcg ggtagaaggc ggatggcacc cgaacaggtg
      901 ccggtgtgca acgccggagg tgcgacctgc ggccaggcgg aagcctgtgt ggaaatgggg
      961 gagtggggga gtatgccggg atcggcctcc ggcgaggcgc gcgagtgcct cgtccgctga
     1021 gcctctcatc tcaaacgcct cccctccgcg gcgcgtctcc gcgtgctgtt cacaccggat
     1081 gggaggcttc cggaatgttc aggcaacgca ttctcagagc atgcttcggc ctgctgctcg
     1141 gcctgcccgt gctcgccgcg gccgggtgcg gctcgggtga tgacgctgat cccatactgg
     1201 gaccggattc cggtcaggtg cgtgccgtgg tcacggtgga tggccagccg cttcctggcg
     1261 tgaatgccgc tctcacagga gatacgacgc gcacggcgac gaccggctcg gatggagtgg
     1321 ccctcttcac cggcctgcca aacggcaact acacggtcac catctccggc ttcgatgcga
     1381 gccgggtcac cttttctcgg acgagcgtcc cggtgacgat ctcggaaagc gtcggtctgg
     1441 tcacggtcga attccagggg atcggcccgt ccacggcggc gatcacgggc gtggtgacag
     1501 tcaatggcca ggcgcccgcg cagctttcgg gccagacggt gacgatcaca ctggtgccgc
     1561 catcgggcga tccggtgacg ggcgacctca cgggcaccac cggtgagtac gccttccgcc
     1621 agcttgcggc ggggcaatac acgctgcgcg tgaccgtggg taacgacgtg cgtgcgacga
     1681 cggtgagcgt cgcggaagga cagcaacttc aggcgaacct ggagttcgcc agcctgccgg
     1741 ctttcaatca gggccc
//
LOCUS       DAOSCV010000958         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45360_length_1753_cov_3.308009, whole genome shotgun sequence.
ACCESSION   DAOSCV010000958 DAOSCV010000000
VERSION     DAOSCV010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45360_length_1753_cov_3.308009"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /gene="mutL"
                     /locus_tag="PLY56_18070"
     CDS             <1..1543
                     /gene="mutL"
                     /locus_tag="PLY56_18070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ23435.1"
                     /translation="HQQAAAEHRGVPQGTQVTVTHLFSNVPARLKFLKTTQTELSHII
                     DLMVRFALSYPGIHFKLAHNGRIVFQSPGSGDPLNTVVAAYGREVAKEMLPVASPEET
                     PVRVRGYTSRPSMTRTSRSHQAFFVNGRLVRSPLLSRALDEAYKGYVPAGRYPVACLF
                     IDVDPAQVDVNVHPTKMEVRFASEREVFSAAVSAIRRALMGVNLAQPAFPTRVEQSIQ
                     AQTVPDDPFADPDEKPAESGKAAPAPDGVREPAVSVPYSPTARPPLPPASCPVEKPPE
                     WRLKPTETQPALTFEDAARAPAREEAPPAVVPAADAPSAPPAEKTTLPNLTPVARVLD
                     TYLICEGPDAIYIIDQHAAHERVIYDALMRREENDIAPQPLLIPLTLNLSHSDAAALE
                     SHREALQELGFEIEPFGRDTYVLRSVPSILVGRNYEAMLRDLLDDLRERERTGRLEEM
                     RSEICALVACHSVTRSGDPLSPEEMARLVQDLRASPSPYTCAHGRPTLLVLSRSDLER
                     QIGRG"
BASE COUNT          300 a          650 c          538 g          265 t
ORIGIN      
        1 gcaccagcag gccgccgccg agcaccgcgg cgtgccccag ggcactcagg tgacggtgac
       61 ccacctcttc agcaacgtgc ccgcccgcct gaagttcctg aagaccacac aaaccgagct
      121 cagccacatc atcgacctga tggtccgctt cgcgctctcc tatcccggca tccatttcaa
      181 gctggcgcac aatggccgga tcgtcttcca atcgcccggc tcgggcgacc cgctgaacac
      241 tgtggtggcc gcctatggaa gggaagtggc gaaggagatg cttccggtgg ccagcccgga
      301 agagacgccg gtccgggtgc gcggctacac ctcccggccc tcgatgacac gcacgagccg
      361 ctcgcaccag gcgttcttcg tgaacggacg gctggtgcgc tcccccctac tctcccgcgc
      421 gctcgacgag gcctataagg gctacgtgcc cgccgggcgc tatccggtcg cctgcctctt
      481 catcgacgtg gatccggcgc aggtggatgt gaacgtgcac cccaccaaga tggaagtgcg
      541 ctttgcttcg gagcgcgagg tcttcagcgc cgccgtgagc gcgatccgcc gcgcgctcat
      601 gggggtgaac ctggcccagc cggcgttccc gacgcgcgtc gagcagagca tccaggccca
      661 gacggtgccc gacgacccct tcgccgatcc ggacgagaag cctgcggaat ccggcaaagc
      721 cgctcctgct ccagacggag tgcgcgagcc agccgtttcc gtgccatact ccccgacagc
      781 gcgcccaccg cttccaccag ccagctgccc cgtcgagaag ccaccggagt ggcgcctgaa
      841 gccgacagag acgcagcccg cgcttacctt cgaagacgcg gctagggcac cggcaaggga
      901 agaagccccg ccggccgtgg tccccgcggc ggacgcgccc agcgctcccc cggccgagaa
      961 gacaacactt ccgaacctga cgccggtggc acgggtgttg gatacctacc tcatctgcga
     1021 gggcccggac gcgatctata tcatcgacca gcacgccgcc cacgagcgcg tgatctacga
     1081 tgccctcatg cgccgcgaag agaacgacat cgcgccccag ccgctcctga tcccgctgac
     1141 gctcaacctc tcgcactccg acgcggcggc cctggagagc caccgcgagg cgcttcagga
     1201 gctcgggttt gagatcgagc cgttcgggcg tgatacctac gtcttgcgca gtgtaccctc
     1261 gatcctcgtc gggcgcaact acgaggcgat gctccgggat ctcctggacg acctgcgcga
     1321 gcgcgagcgc accggtcgcc tggaggagat gcgctcggag atctgcgccc ttgtggcctg
     1381 ccactcggtg acgcgctccg gggatccgct ttcgcccgag gagatggcgc gcctggtgca
     1441 agacctgcgc gcgagtccgt caccctacac ctgcgcccac ggccggccca cgctgctggt
     1501 cctttcccgt tccgatctgg agcgccaaat cggaagagga tagagaaaag ccgccccctc
     1561 ctccagccag gaagcagggc cctgcgtagc gtatacctgt ggcatggctg gacaccatcc
     1621 cggacccatg cggcccgttc ccgagcagaa ggcgggtgca agcgcccgcc aggcgattct
     1681 cacgcgacgc accgcggcca tcttcttgcc gaccatcgcc gtgctggcct acttcaccac
     1741 ctattccgag tgc
//
LOCUS       DAOSCV010000959         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45393_length_1752_cov_2.682970, whole genome shotgun sequence.
ACCESSION   DAOSCV010000959 DAOSCV010000000
VERSION     DAOSCV010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45393_length_1752_cov_2.682970"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLY56_18075"
     CDS             complement(<1..398)
                     /locus_tag="PLY56_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23436.1"
                     /translation="MVRFLLLILCIVGGIGCTGAASAQATLPVTASQATVAIAGRPVL
                     RIRTSLGGVPPEQRAAIVAGRLQELLARGVPPRRIVSRKAGKNAELLYGPTLLLTVTP
                     QEARAQNSSAASLAALWGHRLRALLSTPLLT"
     gene            complement(521..1273)
                     /locus_tag="PLY56_18080"
     CDS             complement(521..1273)
                     /locus_tag="PLY56_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ23437.1"
                     /translation="MAGHSKWHNIRLRKGAQDARKGKIFTKLAREIIVAAKEGGGNPE
                     MNGRLRLAIEKARDASMPVDNIKRAIQRGTGEGGEAVNYQEMVYEGYAPGGTAVMVSV
                     LTDNKNRTVADLRSIFSRNGGSLGETGCVAWMFEQKGVISISTDQVEEDALLEIALEA
                     GADDVRTEDDTFEVLTSPENFSQVKDAIVAAGLRPSSAEVTMLPKSTVSLGGKEAEQV
                     LRLLDMLDDHDDVQQVYANFDISDRELEAAAA"
     gene            complement(1477..>1752)
                     /locus_tag="PLY56_18085"
     CDS             complement(1477..>1752)
                     /locus_tag="PLY56_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOJ23438.1"
                     /translation="IGNRVKIYPGVTLGAKSFPLDAHGNPIKAIKRHPTLEDDVIIYA
                     GATILGGDTVIGQGSVIGGNVWITSSVPPHSTVLHDNPKPIVKKPGT"
BASE COUNT          325 a          560 c          548 g          319 t
ORIGIN      
        1 gtgaggagag gcgtggagag aagagcacgg aggcgatgac cccagagcgc ggccagagac
       61 gctgcacttg agttctgcgc tctcgcttcc tgcggggtga cggtcagaag cagtgtaggg
      121 ccgtagagaa gctccgcatt cttgccggcc ttgcgcgaga cgattcgtcg tggtggtacg
      181 ccacgggcca gcagctcctg caaccgccct gcaacgatgg cggcgcgctg ttcggggggc
      241 accccaccaa gcgaggtgcg aattcgaagg accgggcgac cggcgatcgc gaccgtcgcc
      301 tggctggcgg tcaccggcag cgtcgcctgg gccgaagcgg cacccgtgca cccgatgcca
      361 ccaacgatgc agaggatgag aaggaggaaa cgcaccatga caccattcgt ggtcggggat
      421 gaggccgtgc cgtattcggc tcgcggggcc caaaccgtgg cggagcgcaa aggcaaccac
      481 gggccgcgaa ccgaatacgg ttttgcccca tcagggctga tcaggccgca gccgcttcga
      541 gctcgcgatc ggagatgtcg aagttggcat agacctgctg cacgtcgtcg tggtcatcca
      601 gcatatcgag caggcggagc acctgttccg cttccttgcc acccaacgag acggtcgact
      661 tgggaagcat ggtaacctcc gcgctcgagg gccggagccc cgcagcgacg attgcatcct
      721 tcacctggga gaagttctcg ggcgaagtca gcacttcgaa ggtatcatcc tcggtgcgca
      781 catcgtcagc gccagcctcg agagcgatct cgaggagcgc atcttcctcg acctgatccg
      841 tggagatgga gatcacgccc ttctgctcaa acatccaggc gacacacccc gtttcaccaa
      901 ggctgccgcc gtttcgcgaa aagatgctgc gcagatcggc cacggtgcgg ttcttgttgt
      961 ccgtgaggac actcaccatc accgcggtcc ctcccggggc atagccttcg tataccatct
     1021 cctggtagtt gacggcctcg cccccctcgc cggtaccgcg ctggatcgcc cgcttgatgt
     1081 tatccacagg catgctcgcg tcgcgcgcct tctcaatagc gagccggagg cgaccgttca
     1141 tttcggggtt gccaccgccc tcctttgcgg ccacgatgat ctcacgagcc agctttgtga
     1201 aaatcttccc cttccgggca tcctgggcac ccttgcggag acgaatgttg tgccatttgg
     1261 agtgtccagc catagtatgc gccccgccgc catggacggg atatgcctcc ccccttccgg
     1321 atcgattata gcatgggcta tcgcacgccg tcaaccgaaa gcaagggggc gcctagggcc
     1381 aaaaccacac aaaacagggg ggcgtgcagg gctagaacga cacgcaggtc aagcagggtg
     1441 gcgggcaagc gccttggccg cgctcgcccg cgcggcttac gttcccggct tctttacgat
     1501 tggtttggga ttgtcgtgca gcaccgtgct gtgcgggggc accgacgacg tgatccagac
     1561 gttgccgccg atcacggatc cctggccgat caccgtatct ccgccaagaa tcgtcgctcc
     1621 agcgtagatg atcacgtcat cttccagggt ggggtggcgc ttgatcgcct tgatcggatt
     1681 gccgtgcgca tccaacggaa agctcttggc gcccaacgtc acgcccggat agatcttcac
     1741 gcggttgccg at
//
LOCUS       DAOSCV010000960         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45474_length_1750_cov_2.735693, whole genome shotgun sequence.
ACCESSION   DAOSCV010000960 DAOSCV010000000
VERSION     DAOSCV010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45474_length_1750_cov_2.735693"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PLY56_18090"
     CDS             <1..1216
                     /locus_tag="PLY56_18090"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOJ23439.1"
                     /translation="FGAVDELIPACQDYVRDRARHCLASYYPGVPAHEIEMLLNAPIV
                     TFNAESFLMRADEPCRDVYLVLTGEVELLRGGDRGRRTLSAGGLLGEGAAILRKPVAH
                     TYRATSFVQALRIPANVYLAFVQRNGLLPRIADVRERREFLDRTWLFGDSIAYPTQVT
                     LAREMVPHCLIPGHPTSLNSDPAVCLIREGEVQLLLGENVLETLGPGEFYGESTVLHG
                     TPGFFTIRATMPTELYRIPGGVLRHNPVVRWKLLEVYERRMQHILNPTLVAGPVFRWR
                     PEYSTGISEIDEGHQQLFDAANRFHQSVAAGEGAQVLPWILSFLEEFARMHFAREEEL
                     LREHAYPGYARHHRLHERLLEALEEWKQVFRESAATPYHELVQFFSNWILEHILTEDH
                     KYGRYLNERGVT"
     gene            1349..>1750
                     /locus_tag="PLY56_18095"
     CDS             1349..>1750
                     /locus_tag="PLY56_18095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23440.1"
                     /translation="MQPIGVGIVGAGFSANQHAGAYHCVTGVPVKRVGVVSRTPEKAA
                     AFAKKHGFEFATTNLQELLSRPDVHVIDICAPNFAHEEIAIAAARAGKHIICEKPLTA
                     YFGDPSDPARMREEAMASVERVLDAVRSAGVL"
BASE COUNT          299 a          565 c          566 g          320 t
ORIGIN      
        1 ctttggcgcc gtggatgagc tcattccggc ctgccaggat tatgtgcgcg accgcgcgcg
       61 ccactgcctt gccagctact atcccggagt gccggcccac gagatcgaga tgctgctcaa
      121 cgcgccgatc gtcaccttca acgcggagag cttcttgatg cgcgcggatg agccttgccg
      181 cgatgtctac ctggtgctga ccggcgaggt ggagttgctc cgcggcggtg atcgcgggcg
      241 gcgcaccctc tctgcgggtg gcctcctcgg cgagggcgcg gcaatcctgc gtaagccggt
      301 cgcgcacact tatcgcgcca cctccttcgt gcaggcgctg aggattcccg cgaatgtcta
      361 tctggcgttc gtccagcgca acggtctcct gccgcggatc gcggatgtgc gcgagcggcg
      421 cgagttcctg gaccgcacgt ggctttttgg cgattctatc gcctacccga cacaggtcac
      481 tctggcccga gagatggtgc cgcattgcct aatacccggc catcctacct cgctgaacag
      541 cgatccggcc gtgtgcctca tccgggaggg cgaggtgcag ctcctcctgg gcgagaatgt
      601 gctcgagacg ctcgggccag gcgagttcta cggcgagtcc accgtcctgc atggcacccc
      661 ggggttcttc accatccggg cgacgatgcc aaccgagctt taccgcattc cgggcggtgt
      721 tctgcggcat aacccggtcg tgcgctggaa gctccttgag gtctacgagc ggcgcatgca
      781 acatatcctc aacccgacgc tggtcgcggg ccccgtcttt cgctggcgtc cggagtactc
      841 gacgggcatt tccgagatcg acgagggcca ccagcagctt ttcgacgcgg ccaatcggtt
      901 ccatcaaagc gtcgccgccg gagagggcgc gcaggtactt ccgtggatac tcagtttcct
      961 ggaggagttc gcccggatgc acttcgcccg cgaagaggaa ctgctgcgcg agcacgctta
     1021 tcccgggtac gcgcgtcacc atcgcctgca cgagcgtctc ttggaagcac tggaagagtg
     1081 gaagcaggtt ttccgggaga gcgcggcgac gccctatcac gagctggtcc aattcttcag
     1141 caactggatc ctcgagcaca tcctgaccga agaccacaag tacgggcgct acctcaacga
     1201 gcgtggcgtc acctagaacg acgcctctcc cggcgcgcgg gcctagcagc ccgcgccccg
     1261 tccgatttcc aggcaaagct ggcccccggt tgacaggcct gcgcccacct cctataatca
     1321 gcgcaaacag atgccgaggg aggaagcaat gcagccgatt ggtgtgggga ttgtgggcgc
     1381 ggggttctcc gcgaatcagc atgcgggcgc ataccactgc gtgaccgggg tgcccgtgaa
     1441 gcgagttggg gtcgtctcgc gaacgcccga gaaagccgcc gcgttcgcga agaagcacgg
     1501 ctttgagttc gcgaccacga acctgcaaga gcttctgagc cggccggacg tgcatgtgat
     1561 cgatatctgc gcgcccaact tcgcgcatga ggagatcgcg attgcagcgg cgcgcgcggg
     1621 gaagcatatc atttgtgaga agccactcac cgcctacttt ggcgacccgt cggatcccgc
     1681 gcgcatgcgc gaggaagcga tggcatcggt cgagagagtc ctggatgcgg tgcgtagcgc
     1741 cggggtgctc
//
LOCUS       DAOSCV010000961         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45514_length_1749_cov_2.595041, whole genome shotgun sequence.
ACCESSION   DAOSCV010000961 DAOSCV010000000
VERSION     DAOSCV010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45514_length_1749_cov_2.595041"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..>1749)
                     /locus_tag="PLY56_18100"
     CDS             complement(227..>1749)
                     /locus_tag="PLY56_18100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ23441.1"
                     /translation="DHLGVPLSEIKVRLGDAIEIGDRRIPINESGEMVINYYGPFRGS
                     LPLPSYIDVVESKYEPRYFRDKIVILGNTLSGGFDIQHTPFGGQYPGVLAQATVVQNI
                     LDGSAIREARPVFGWLLALAGALAMGLTRALSFRSQWITALLLGLAIVVSGALLLYYQ
                     FLWIEMVRPLLAVFVAFVGVSVSTFREASLARARVQGAVDALAEVAGAIAHARNPAEL
                     CQALETGIGRVLGAESAELRIHDAWMRERLSLAEAPPPAPASREGHLPLHLRGKEVGY
                     VSLGPLSADADPALVSAVANFVALALENGAFFEESRDHFLSLTLTLADIIESNDQYTA
                     GHCARVMEYASAIGERLGLSALELEELRYGALLHDVGKTVLDKPVLNKPGALSPEEMA
                     EVRRHPELGRQWLQREKRLQGVSELVGTHHERWDGAGYPRGLAGEEIPLGGRILAAAD
                     VWDALTTDRPYRRALTFEEARRFLVEGRGTQFDPRVVDAFLAYLAETHEALPRAQG"
BASE COUNT          317 a          617 c          549 g          266 t
ORIGIN      
        1 ctccgccgcc gagcggatca tggggcgcga gctggatctt caggtcggcg atgttgccag
       61 ccggctggag gccaacctgc gccgcttcct ctccgagggc cgctgacgcc ggcgaccagc
      121 gttcagccgt tcttaccccg ctggctccac ctgcacagaa accgcagggc ggagccggcg
      181 gggggtaagc ctggtatcca tgcctgcgga tgccggattc ctacccttac ccttgcgcgc
      241 gggggagtgc ttcgtgcgtc tccgcgagat acgccagaaa ggcatcgacg acgcgggggt
      301 caaactgcgt gccgcgaccc tcgaccagga agcggcgggc ttcctcgaat gttagcgccc
      361 gccggtaggg ccggtcggtc gtaagggcat cccacacgtc agccgccgcc agaatgcgcc
      421 ctcccagcgg gatctcctcg cccgccaggc cccgcgggta gccggcgcca tcccagcgct
      481 cgtggtgcgt ccccaccaac tcactcaccc cttgaagccg cttctcccgc tgcagccatt
      541 gccgacctag ctcaggatgc cggcgcacct ccgccatctc ctctggcgag agcgcgcccg
      601 gcttgttcag aaccggctta tcgagcacgg tcttccccac atcatgcagc agcgcgccat
      661 agcgtagctc ctccagctcc agggcagaaa gccccaggcg ctcgccgatg gcagaggcat
      721 actccatgac gcgggcacaa tgaccggcgg tgtattggtc gttcgattca atgatgtccg
      781 ccagggtcag tgtcaggctc aggaaatggt cgcggctctc ctcgaagaag gcaccattct
      841 cgagggccaa cgcgacgaag ttggcaacag cggagacgag cgcaggatcc gcatccgcgc
      901 tcagaggccc gagggagacg tagcccacct ctttgccacg caggtgaagt ggcaggtggc
      961 cctctctcga agcaggcgcc ggcggcggtg cctcagcaag cgagaggcgc tcgcgcatcc
     1021 aggcatcgtg gatacgcagc tccgcgctct ccgcgcccag cacccggccg atccccgtct
     1081 ccagcgcctg acacaactcg gcagggttcc gcgcgtgcgc gatcgccccg gctacctcgg
     1141 ccagagcatc caccgcgccc tgcacgcgcg cccgcgccag actcgcctcc cggaacgtgg
     1201 agacgctgac gcccacaaag gccacaaaga cggccaggag cgggcgcacc atctcgatcc
     1261 acaggaactg gtaatagagg aggagcgccc cagataccac gatcgccaat cccagcagca
     1321 gggccgtgat ccattggctc cggaatgaca gggcgcgtgt caggcccatc gccagcgcgc
     1381 cggcaagagc aagcaaccac ccgaacactg gcctggcttc ccggatggcg gagccatcca
     1441 gaatattctg caccaccgtg gcctgagcga gcacgcccgg atactggccc ccgaacggcg
     1501 tgtgctggat atcgaagccc ccggagagcg tgttgcccaa gatgacgatc ttgtcgcgga
     1561 agtaacgcgg ctcgtacttg ctttcgacca cgtcgatata ggaggggagc ggcagcgagc
     1621 cccggaaggg gccgtagtag ttgatgacca tctcgccgct ctcgttgatc gggatgcgcc
     1681 ggtcgccaat ctcgatggca tcgcccaggc gcactttgat ctcactgagc ggcacgccca
     1741 ggtggtcgg
//
LOCUS       DAOSCV010000962         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45590_length_1747_cov_3.036643, whole genome shotgun sequence.
ACCESSION   DAOSCV010000962 DAOSCV010000000
VERSION     DAOSCV010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45590_length_1747_cov_3.036643"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1504)
                     /gene="hisS"
                     /locus_tag="PLY56_18105"
     CDS             complement(182..1504)
                     /gene="hisS"
                     /locus_tag="PLY56_18105"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ23442.1"
                     /translation="MKMANPPQVLRGMRDFLPDRMILRHHVIGIFRSVFERYGFEPLD
                     TPAIEYAETLEGKYGEEADKLIYKFEDRGGRQVGLRYDLTVPLARVVALYPDLPKPFK
                     RYQIAPVWRAERPQKGRYREFWQCDVDTVGSSSMLADAEILAVLHDSLTALGFTQFVI
                     RINNRKLLKGLSTFAGVPEGEEANVLRSVDKLDKIGEVGVRTELLEKGFDEEVVARLF
                     RLLEPGDDTSDLIARLRVQLAESPDAQEGLAELEQLREYLANLGVRESCYRFDLSMVR
                     GLDYYTGPIFETVVEEPRIGSITGGGRYDHLVGIFSGRDIPAVGSTLGLERIVDVMEA
                     LHLFPETKTLTQVLVAAFDAETIPEALRLATELRRAGTRVEVPLMEKGGFRKFLSYAN
                     AKGIPTVVLLGPDEIARGEVTVRNMADGSQESVPRAEAVARLSGQRAQ"
BASE COUNT          312 a          600 c          550 g          285 t
ORIGIN      
        1 atcgagcccc ggcacgtaga ggccgcgatc gcggtacacg cagagatccg tcgcgaccgg
       61 ttcagattgc ttccggcgtg ccatcaccct cctccatcca acacgcgcgc cagtccccgg
      121 caccggatag ggccggggac tggcgcgcgt gttgctcgct ccccctccca ctcgcgggga
      181 atcattgggc gcgctggccg ctgaggcgag cgacggcctc ggcgcgtggc accgactcct
      241 ggctcccatc cgccatgttg cgcacggtca cctcgccgcg ggcgatctcg tccgggccga
      301 ggaggaccac cgtggggatg cccttcgcgt tggcgtagga gagaaacttc ctgaagccgc
      361 ccttctccat caggggcact tcgacgcgcg tgcctgcgcg gcgcagctcc gtggccaggc
      421 ggagcgcctc cgggatcgtc tcggcgtcga acgcggcgac aagcacctgg gtaagcgtct
      481 tcgtctccgg gaagaggtgg agggcctcca tcacgtccac gatccgctcc aggccgagcg
      541 tggagcccac ggcagggatg tcgcgcccgc tgaagatacc caccagatgg tcgtagcgcc
      601 cgcccccggt gatgctcccg atgcgcggct cttcgacgac ggtctcgaag atcggaccgg
      661 tgtagtagtc cagaccgcgg accatggaaa gatcgaaccg gtagcagctc tcgcgcacgc
      721 ccaggttcgc caggtactcg cggagctgct ccaactcggc caggccttcc tgggcgtcag
      781 ggctctctgc cagctgaaca cgcagccggg caatgagatc gctcgtgtcg tccccgggct
      841 ccaggaggcg gaagaggcgc gcgaccacct cctcatcgaa gcccttctcc aacaactcgg
      901 tgcgtacccc cacctcgccg atcttatcga gcttatcgac gctgcgcagg acattcgcct
      961 cttcgccctc gggcacgccg gcgaaggtgg aaaggccctt gagcagtttc cggttattga
     1021 tccggatcac gaactgcgtg aagcccagcg cggtgagcga gtcgtggagg acagcgagga
     1081 tctcggcatc ggcgagcatg ctggacgagc cgacggtatc gacatcgcac tgccagaact
     1141 cgcgataccg gcctttctga gggcgctccg cgcgccagac aggcgcgatc tggtagcgct
     1201 tgaacggctt gggcagatcc ggatagaggg cgacgacgcg cgccaggggc accgtcagat
     1261 cgtagcggag gcccacctgc cggccgccgc ggtcttcaaa cttgtaaatc agtttatcgg
     1321 cttcctcgcc gtacttgcct tccagagtct cggcatattc aatggcggga gtatccagtg
     1381 gctcgaaacc atagcgctcg aagacagagc gaaagatgcc aatcacgtgg tggcgcaaga
     1441 tcatccggtc gggcaagaaa tcgcgcatgc cgcgcagtac ctggggcgga ttcgccatct
     1501 tcatccactt ccgaagatgc acggggccac agccccctgc aactcacggc ctcctctcac
     1561 gtttcgtgcc gtcattgtag caccaggcag ggcttccgtc aaacgcctgg aggcaggcgg
     1621 ccgtttgcct aacccccagc cccttcccta ccctccggcc ccacgccggc cacagggaag
     1681 gggagcgacc gcgtggcgct ctcgaaggac gccgaaggcg tccaacaaca cagaccaggt
     1741 tcaggag
//
LOCUS       DAOSCV010000963         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45602_length_1747_cov_2.377660, whole genome shotgun sequence.
ACCESSION   DAOSCV010000963 DAOSCV010000000
VERSION     DAOSCV010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45602_length_1747_cov_2.377660"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLY56_18110"
     CDS             complement(<1..654)
                     /locus_tag="PLY56_18110"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HOJ23443.1"
                     /translation="MLGRHAALEIVYHAMELTKADQVQVSLVTSEEALTRFANSAIHQ
                     NVAERDAVLGIRAVIGKRVGYVASNATDSEGIRRLAERARAVTLAQEENPDFVSLPGP
                     TRVPALDIWRERTASFTPDDRAQAVRVVVDTAGSWGAIASGAFSTWEREHAVVNSLGV
                     EAYCASTGAMLSTVMSKEPGGFGYASALAQDVGAIDPSAVAHEAAMVCARSVSPRELA
                     "
     gene            complement(659..>1747)
                     /locus_tag="PLY56_18115"
     CDS             complement(659..>1747)
                     /locus_tag="PLY56_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOJ23444.1"
                     /translation="PLEEKISLLLDADAALRREPRVTVSEARMAFFREEKTFASTEGA
                     YLEQVKTESGAGVAATATESGEAQTRSYPAAFGGDFAAAGYEFIESLDLEQGAETAGQ
                     EAAALLDAPQCPSGVTDLILEGQQLALQIHESCGHPVELDRVFGTEASYAGTSFLTTD
                     KLDTFQYGSDAVTLVADATIEGGLGSFAYDDEGVPAQRVPLVEEGIFRGYLSSRETAA
                     RIGRTSSGAMRADGWNRIPLIRMTNINLEPGDWSLDEMIEETQEGLLLSTNRSWSIDD
                     KRLNFQFGTEAAWEIRNGRLGRLLKNPTYTGITYQFWRACDAVARREEWRVWGIPNCG
                     KGEPCQVAHVGHGASAARFRGVRVGVRR"
BASE COUNT          269 a          619 c          570 g          289 t
ORIGIN      
        1 ggcgagctct cggggagaga cgctccgcgc gcacaccatc gcggcctcgt gcgcgacggc
       61 gctcggatcg atggcaccca catcctgcgc gagggcgctt gcatacccga agccgcccgg
      121 ctccttgctc atcaccgtgg agagcatcgc tccggtgctc gcgcaatagg cttccacccc
      181 cagggagttc acgacggcat gctcgcgctc ccaggtagag aaggctccgc tggcgatagc
      241 accccacgat ccggccgtat cgacaactac ccgcacggcc tgggcgcggt cgtccggcgt
      301 gaagctcgcc gtgcgctcgc gccagatgtc cagggcgggc acgcgcgtcg gccccggcag
      361 cgagacaaag tcggggttct cctcctgggc cagggtcacg gcccgggcgc gctcagccag
      421 ccgacggatc ccctcgctat ccgtggcgtt gctggccacg tagccaaccc gcttcccgat
      481 cacagcacgg atgccgagga cggcgtcgcg ctccgccacg ttctggtgga tggccgaatt
      541 ggcaaaccgg gtgagggctt cctcgctggt gacgagggag acctgcacct ggtccgcctt
      601 ggtcagctcc atcgcgtggt agacgatctc cagtgctgcg tgtcgtccca gcatgctctc
      661 acctcctcac gccaacgcgc acgccgcgga agcgcgccgc cgaggcccca tggcccacgt
      721 gggccacctg gcacggctcg cccttcccgc agttcggaat cccccaaacg cgccattcct
      781 cccggcgcgc gacggcatca catgcgcgcc aaaactgata ggtgatcccg gtgtaggtcg
      841 ggttcttcag gaggcggccc aggcggccgt tccggatctc ccaggcggcc tcggtgccaa
      901 actggaagtt gaggcgcttg tcgtcgatgc tccacgaccg gttggtcgac aggagaagcc
      961 cctcctgcgt ctcctcgatc atctcgtcca gcgaccagtc gcccggctcc aggttgatat
     1021 tggtcatccg gatgagcggg atgcggttcc atccatcggc gcgcatggca ccgctgctcg
     1081 tccggccgat acgcgccgcc gtctcgcgcg atgagaggta gccgcggaag atcccctcct
     1141 ccaccagcgg cacgcgctgc gccggcaccc cttcatcatc gtaggcgaag ctccccagtc
     1201 ccccctcaat cgtggcatcg gcgactagcg tgaccgcgtc cgagccgtac tggaaggtat
     1261 ccagcttgtc cgtcgtcagg aaggaggtgc ctgcgtaaga ggcttcggtg ccgaagacgc
     1321 ggtccagctc cacggggtgg ccgcacgatt cgtggatctg gagcgcgagt tgctggcctt
     1381 ccaggatgag atcggttact cccgaggggc actgtggggc atcaaggagc gcggcggcct
     1441 cctggccggc ggtctcggcg ccttgctcca ggtcgaggct ctcgatgaac tcatagccag
     1501 cggcggcgaa atcaccccca aaggccgccg ggtaggagcg ggtctgggcc tcgccgctct
     1561 cggtggcggt cgcggccaca ccggcgccgc tctccgtctt cacctgctcg agataggctc
     1621 cctccgtgct ggcaaacgtc ttctcctcgc ggaagaaagc catgcgtgcc tcggagacgg
     1681 tgacgcgcgg ctcgcggcgc agggcggcat cggcatccag gagaagggag atcttctcct
     1741 cgagcgg
//
LOCUS       DAOSCV010000964         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45719_length_1743_cov_3.290284, whole genome shotgun sequence.
ACCESSION   DAOSCV010000964 DAOSCV010000000
VERSION     DAOSCV010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45719_length_1743_cov_3.290284"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="cheB"
                     /locus_tag="PLY56_18120"
     CDS             complement(<1..866)
                     /gene="cheB"
                     /locus_tag="PLY56_18120"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOJ23445.1"
                     /translation="MRIAIVNDLPMAVEILRRVVLSVPGYEVAWIARNGAEAVEKCAA
                     DRPDLILMDLIMPVMDGVEATRRIMKETPCAILVVTATVAGNAAKVFEAMGLGALDAI
                     ATPAYVPGEGLRGDSELLKKIATIQMLISGARAPAAAASAPAPTPLIAIGASTGGPAA
                     LVDVLSRMPHDAGAAIVVVQHVDAQFAPGLAAWLGEQCVLPVVLAREGERPARNTVYL
                     AGTNDHLVFGADQAFHYTREPTDYPYRPSVDAFFLSVKRHWQTKGAAILLTGMGRDGA
                     TGLLALRQAGWHT"
     gene            complement(873..>1743)
                     /locus_tag="PLY56_18125"
     CDS             complement(873..>1743)
                     /locus_tag="PLY56_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ23446.1"
                     /translation="AFHLLLPLTVSVVRALLFEISGETYAIPFTRIDRCLRVPLEEVR
                     TVEDRQYCTVDGAHVGIVEATQALGLPPVPRESRVLNTIIISDRLTRYGLVVDRFCGQ
                     RELVVVPLDRRLGKLQNISAGAVLQDGSPVLILDTEDLVRSIDKLLSAGGLQKLRAGV
                     EQSGPKRKRVLVVDDSLTVREVERRLLENGGYDVTVAVDGMDGWNALRSGNFDLLLTD
                     VDMPRMDGFELVRRVRAAPQTSSLPVIIVSYKDQEEYRIKGLEAGANAYLTKGSFHGE
                     GLLKTVRDLIGEA"
BASE COUNT          303 a          579 c          563 g          298 t
ORIGIN      
        1 gtgtgccagc ctgcctggcg aagggcgagc aggccggttg cgccgtcgcg gcccattccg
       61 gtgagcagta tggcggcgcc tttagtttgc cagtggcgct tcacgctgag gaaaaaagca
      121 tccaccgagg ggcggtaggg gtagtcggtg ggttctcgag tatagtgaaa tgcctggtcc
      181 gcgccgaata cgaggtgatc gttggttccc gcaagataaa cggtgtttcg cgccgggcgc
      241 tcgccttctc gcgccagcac cacgggtaat acacactgct cgccgagcca tgccgcgagc
      301 cctggagcaa actgcgcgtc cacatgctgc accacgacga tggccgcccc ggcgtcgtgc
      361 ggcatgcgcg acagcacgtc gaccagagcc gcaggtccgc cagtcgaggc gccgatggcg
      421 atcaggggcg tgggcgcggg agcggatgcc gcagccgcgg gtgcgcgtgc cccagagatg
      481 agcatctgaa tggtggcgat cttcttgagt aactcgctat cgcccctgag accctcgccc
      541 ggcacgtagg ccggagttgc gatggcatcg agagccccca gtcccatggc ctcgaagacc
      601 ttggccgcat tgccggccac tgtggcggtc actaccagga tcgcgcaggg cgtctccttc
      661 ataatgcgcc tcgtggcctc cacgccgtcc atcaccggca tgatgaggtc catgaggatg
      721 aggtccggcc ggtccgcggc gcatttctcg acggcctcgg cgccgttgcg cgcgatccag
      781 gccacttcat agcccggaac tgaaaggacc acccggcgca gaatctcaac agccatcggg
      841 aggtcgttga ctatggcgat gcgcatcgct agctaggcct caccgatcag gtcccgcacg
      901 gtcttcagca ggccctctcc atggaaactg cccttcgtca ggtaggcgtt ggctcccgct
      961 tcgagtccct tgatccggta ctcttcctgg tccttgtagg agacgatgat cacgggaagt
     1021 gaactggtct ggggcgcggc gcggacgcgc ctcaccagtt cgaagccgtc catgcgcggc
     1081 atgtccacgt cggtcaacag cagatcgaag ttgcccgaac gcagggcgtt ccacccgtcc
     1141 attccatcca ccgccacggt cacgtcgtat ccgccattct cgagcagccg ccgctcgacc
     1201 tcgcgcaccg tgagggagtc gtccaccacg agcacgcgtt tgcgcttcgg gccgctctgc
     1261 tccacaccgg cgcggagctt ctgcagcccg ccggccgaga gcagcttatc gatgctgcgc
     1321 accaggtcct cggtatcgag gatcaaaacc ggcgaaccat cctgcagcac ggcgccggcg
     1381 ctgatattct gcagcttccc cagccgccgg tcaagcggta cgacaaccaa ttcgcgctgc
     1441 ccgcagaagc gatctaccac caggccgtag cgcgtcagcc ggtcgctgat gatgatggtg
     1501 ttcagcaccc tgctctcgcg gggcactggg ggcagcccga gcgcctgggt ggcttccaca
     1561 atgccgacgt gggcgccgtc cacggtgcag tactggcggt cctcgacggt gcgcacttcc
     1621 tcgagcggca cgcgcaggca acggtcgatg cgggtgaacg gaatggcgta ggtttcgccg
     1681 gagatttcaa acagcagcgc gcggacgacc gagaccgtga gcggcagcag caggtgaaac
     1741 gcc
//
LOCUS       DAOSCV010000965         1743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45741_length_1743_cov_1.500000, whole genome shotgun sequence.
ACCESSION   DAOSCV010000965 DAOSCV010000000
VERSION     DAOSCV010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45741_length_1743_cov_1.500000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLY56_18130"
     CDS             complement(<1..338)
                     /locus_tag="PLY56_18130"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ23447.1"
                     /translation="MGRTVIEAVMGADDMTLVGGVDPGYAGQLLSDLVPGAPQVPVAD
                     SVQVGIDAWAPEVLVDFTRPEVVIENLRVALAAGVACVVGTTGFTAEALDEVRHLCRQ
                     HGTPALIAPN"
     gene            complement(529..>1743)
                     /gene="pnp"
                     /locus_tag="PLY56_18135"
     CDS             complement(529..>1743)
                     /gene="pnp"
                     /locus_tag="PLY56_18135"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ23448.1"
                     /translation="LFTRGETQVLSLTTLGATRDQKLVRSLEQEEYSRFMHHYNFPPF
                     CTGEAKPLRGASRREIGHGALVSRSLERMLPPEEEFPYTIRVVSEVLESNGSSSMASA
                     CASSMSLMDAGAPIKAAVAGISVGLVYESEDNYLLLTDIQGLEDAAGDMDFKVTGTAE
                     GVNGLHLDIKVHGLPSRVLAEGLERARQARLQILEVMNRTISAPRPELSKYAPRMFTI
                     HVPKDAIGMVIGPGGKNIRRLEEECEVKIDIEDDGLVMIFGENVEGCERARQQISDLT
                     REIEVGEVFTGRVVNITDFGAFVELLPGRDALLHISDLEHKRTERVEDVLKVGDEVRV
                     KVIELEPGGKIKVSRKALLERDANAGEDDRGGRSGDQSARGGGHSRGSGRDRRGEGRG
                     SGGSAYYRDKRR"
BASE COUNT          287 a          629 c          521 g          306 t
ORIGIN      
        1 aagttggggg cgatcagggc cggagtgccg tgctggcggc acaggtggcg cacctcgtcc
       61 agagcctccg cggtaaagcc ggtggtaccc accacgcagg ctaccccggc ggccaaggcc
      121 acccgtagat tctcaatgac cacctccgga cgcgtgaagt ccaccagcac ctccggcgcc
      181 cacgcgtcta tgcccacctg cacactatcg gccaccggca cctggggcgc ccccggcact
      241 aggtccgaga gcaactgccc ggcgtagccg ggatccacac ctcccaccag cgtcatatcg
      301 tcggcgccca tcaccgcctc gataacagtg cgccccatac gtcctcccgc cccggacacc
      361 atcacctgca actgcgacat cgcactcacc cgatcttgag ccgctgaatt cctcccgctg
      421 tcagcccgcg gtaatccggt acattcgaca tgcgcctccg ctcacctgca ggggcgtaca
      481 ttaatcagcc ggggtctgat tagaccccgg ctggtcgttc gcccccggtt atcggcgctt
      541 gtcgcggtag taggcactgc ccccagagcc ccggccttcg ccgcgccggt cccgtcccga
      601 gccgcgactg tgcccaccgc cacgagcgct ctggtcccca gagcggcccc cgcggtcgtc
      661 ttccccggca ttggcgtcgc gctccagcag cgccttgcgg cttaccttga tcttgccgcc
      721 gggctccagc tcgatgacct taactctgac ctcatcgccg accttgagca catcctccac
      781 ccgctcggtg cgcttgtgtt cgagatccga gatgtgtagc agagcatctc gtcccggcag
      841 cagctcaacg aaagccccga agtcggtgat gttgaccacc ctgccggtga atacttcgcc
      901 gacttcgatc tcgcgggtca ggtccgagat ctgctggcgg gcgcgctcgc agccctcgac
      961 gttctcgccg aagatcatga ccagcccgtc gtcctcgatg tcgatcttga cctcgcactc
     1021 ctcttcgagg cgacgaatgt tcttgccgcc tggaccaatg accatgccga tggcgtcctt
     1081 gggcacgtgg atggtgaaca tgcgcggggc gtacttggac agctccggtc gcggcgcgga
     1141 gatggtgcga ttcatgacct cgaggatttg caggcgcgcc tgccgggcgc gctccagacc
     1201 ttcagccagc acccgcgagg gcaggccgtg caccttgatg tccagatgca gcccattgac
     1261 gccctccgcc gtgccggtca ccttaaagtc catatccccg gccgcgtcct ccagcccctg
     1321 gatgtcggtg agcaacaggt aattgtcttc gctctcgtaa accaacccca cggagatgcc
     1381 ggccactgcc gccttgatgg gcgcacccgc gtccatcagc gacatggagc tggcgcaggc
     1441 ggaggccatg gaactggagc cattgctctc caggacttcg gagaccacgc ggatggtgta
     1501 agggaactcc tcctctgggg gcaacatgcg ctccagggag cggctgacca gcgcgccgtg
     1561 cccgatctcg cgccgcgagg cgccgcgcag cggtttggcc tcgccggtgc agaagggcgg
     1621 gaagttgtag tggtgcatga agcgcgagta ctcctcctgc tccagcgagc gcaccagctt
     1681 ctggtcgcgg gtggcgccca gcgtggtcag agagagcacc tgggtctcac cgcgggtgaa
     1741 caa
//
LOCUS       DAOSCV010000966         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45786_length_1741_cov_2.943060, whole genome shotgun sequence.
ACCESSION   DAOSCV010000966 DAOSCV010000000
VERSION     DAOSCV010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45786_length_1741_cov_2.943060"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLY56_18140"
     CDS             complement(<1..367)
                     /locus_tag="PLY56_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ23449.1"
                     /translation="MIYDVHVHIGRHVPPLEGGSGDAVTDLVERALLHGIDRQCISSL
                     GNRGYIIDPTLEEVREANDHVLEAMAAHPNHLLGFCYVNPRHGGDALSEIDRCVARGG
                     MVGIKLWVACKASDPLVDPI"
     gene            complement(483..>1741)
                     /locus_tag="PLY56_18145"
     CDS             complement(483..>1741)
                     /locus_tag="PLY56_18145"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ23450.1"
                     /translation="RENPNLRRLWYGQVVSQMGDYFSTVAVMALLIERSGERAGTGVA
                     LYTVIRHLPLFLLGPAAGIAADRLDRRRILIVTDLLRGVLALAYLVTWAGGPLWVLYA
                     ATAGLAGISLFFNTARGALLPTITTRNQLMVANALSAATYGTTLAVGSLIGGVIAGVL
                     GRGTAFVLNALSFLLSAVIIAKIQAPARQREASAGAIAVLGAMHRDIVEGFVAVRRHP
                     ALRGLLFLPAAWALGTGAARILYSLYGASLGLETMGELVRNPRDFGIAILYAAMGTGA
                     VAGTFLARRMATRPAEDLVRAIPRALFVDGLGLAALSIVPNLAGAFIVLLIREVGYAL
                     WWTSHQTAVMLLAPDTMRGRVFAAQESLMTLALLLSMLTAGPAVDQGGLRAVALAAGG
                     IIIAASILFALWSRTLPRKEPGHFSR"
BASE COUNT          328 a          534 c          624 g          255 t
ORIGIN      
        1 ggatcggatc gaccagtgga tccgatgcct tgcacgcaac ccacagcttg atccctacca
       61 tgccgccgcg ggccacacag cggtcgatct cggacagagc gtcgccgccg tggcgcgggt
      121 tgacgtagca gaacccgagg aggtgattgg gatgagccgc catcgcctca aggacgtggt
      181 cgttggcctc tcgcacttct tcgagggtcg ggtcgatgat gtagccccgg ttacccagcg
      241 agctgatgca ttgacgatcg atgccgtgga gcagcgcgcg ctcgacgagg tcagtcaccg
      301 cgtcgccgct tccgccctcc agtgggggca cgtgacgccc gatgtggacg tggacgtcgt
      361 agatcattac cgtatctcct cacgagagca gtgttgttgc tctcgtgaca ggtttctccc
      421 aaactgcgcc aaccccttgt ggagatcgca tcgcgtttga gcgattttcc gacaggcacc
      481 gcctatcgcg aaaagtgccc gggctccttg cgcggaagtg tccggctcca aagggcaaaa
      541 aggatcgatg cggcgatgat gatccctccc gcagccaggg cgaccgctcg aagcccgccc
      601 tggtccaccg cgggcccggc ggtgagcatc gaaaggagaa gggccagggt catgagcgat
      661 tcctgggcgg cgaagacgcg cccgcgcatc gtatcgggcg ccaggagcat gaccgcggtc
      721 tgatgggaag tccaccagag ggcatagccg acctcgcgga tcaggagcac gatgaaggct
      781 cccgcgaggt tggggacgat gctgagcgcg gcgagcccaa gaccatccac gaagagcgcc
      841 cggggaatgg cgcggacgag gtcctctgcc ggtcgggttg ccatcctccg ggccaggaag
      901 gtgcctgcga cggcgccggt gcccatcgcc gcatagagga tggcgatgcc gaagtcacgt
      961 gggttgcgca ccagctcgcc catcgtttca agtccaaggg atgcgccgta gaggctatac
     1021 aggattcgcg cggcgccggt gccgagcgcc caggccgcgg gaaggaagag cagcccccgg
     1081 agtgccggat gtcgtcgaac agcgacgaag ccctcgacga tatcgcggtg catggcgccc
     1141 agcacggcga tggcgcccgc ggaggcctcc cgctggcggg ccggcgcctg gatcttcgcg
     1201 atgataacgg cggagaggag gaaggagagc gcgttcagga cgaaagcggt gccgcgtcca
     1261 agcactcccg cgatcacccc accaatgagc gacccgacag cgagtgtcgt gccgtaagtc
     1321 gccgccgaaa gggcgttggc gaccatgagc tggttgcgtg tggtaatcgt gggcagcagc
     1381 gcgccccggg ccgtattgaa gaagagggag atgcccgcca ggcccgcagt ggccgcgtaa
     1441 agcacccaca ggggaccacc ggcccacgtg acgaggtagg cgagtgccag taccccgcgg
     1501 aggagatcag tgacgatgag gatacgccgg cggtcgagac ggtcggcggc gatgcccgcg
     1561 gccggtccga ggaggaagag gggaaggtgg cggatgacgg tgtagagggc gacgcctgtt
     1621 ccggcgcgct ccccggagcg ttcgatgagg agggccatca cggcgaccgt ggagaagtag
     1681 tcgcccatct gggagacgac ctggccatac cagagccggc gcaggttcgg gttctcgcgc
     1741 a
//
LOCUS       DAOSCV010000967         1741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45806_length_1741_cov_2.024911, whole genome shotgun sequence.
ACCESSION   DAOSCV010000967 DAOSCV010000000
VERSION     DAOSCV010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45806_length_1741_cov_2.024911"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PLY56_18150"
     CDS             <1..507
                     /locus_tag="PLY56_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ23451.1"
                     /translation="LYVAFTCAEPQIQAMRRTGQKRDGALWNLDCVELFLDPESMGTK
                     FLHFIAVPDDDARYDARRGYIEDPVHPLYDREDVSWNAEWRHTYVVDEANKRWSVEME
                     LPFASLGIAPPAPGTRWKGNFGRERYAGLKGGNDPELYLWSPNELGTGFCEPLCFGEI
                     RFEAAGAR"
     gene            718..>1741
                     /locus_tag="PLY56_18155"
     CDS             718..>1741
                     /locus_tag="PLY56_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOJ23452.1"
                     /translation="MKRGRGGRRVLVTAVFLAMLAVGLPAPLLARVDVPDAETLAPLH
                     YDFRRKGPPIKREDRWEELTRGVNLALGKPYRFDRRPNYGVTRDEGDARQLTDGKLVP
                     GDKIWFSKEAVGYQGADPPATICIDLGQVYPIDAVVARVQGGGAWEGSFRYPLQFDVY
                     VSDDDRTYTRVDSVRKREYRDQRGTLFDLPESVGAQPPGNPRPHPFHFGRLRTRGRYV
                     ALRMTLASAYGALDEIAVMKGDHDPAKVTFDPQQATTLLFDAVELLYPRDRMRVPTNV
                     AGGFSFAVRDSRRDTRQPVTFHIDVPAAVTLSWSGSASLRSQEHARDGRPYREYTLTT
                     VPSGGKL"
BASE COUNT          280 a          564 c          606 g          291 t
ORIGIN      
        1 ctctacgtgg cgttcacgtg cgccgagccg cagatccagg cgatgcggcg caccggacag
       61 aagcgcgatg gcgcgctctg gaatctggac tgcgttgagc tgttcctcga tcccgagagt
      121 atgggcacga agttcctgca cttcatcgct gtgcccgacg acgatgcccg ctatgatgcc
      181 cgccgagggt atatcgaaga ccccgtgcac ccgctctacg accgtgagga tgtgagctgg
      241 aacgcggagt ggcggcacac gtacgtggtg gacgaagcga acaagcgctg gagcgtggag
      301 atggagctcc cctttgccag ccttgggata gccccgcctg ctcctggcac gcgctggaag
      361 ggcaactttg gccgcgagcg gtatgcgggc ctgaaggggg gcaacgatcc ggagctttac
      421 ctgtggtcgc ccaacgagct gggcaccggc ttctgcgagc cgctctgctt cggggagatc
      481 cgcttcgagg cggccggcgc gcgctgagca aagagacgcc cggcgttccc gacgggtggc
      541 cgggagttcc cggggaagag aggccacgtg taaaggggtg ctgttccatg gtgtgatcgg
      601 acacatggcg tgtctgtggg gggagccacg atggagagct gcttgattcg agggcggggc
      661 actgagggcg gaggcggggg gggccctgcc gcgcgccgct gctcgacgag acgatgggtg
      721 aagagaggcc gggggggccg ccgcgtcctg gtcacggcgg tgttccttgc catgctagcc
      781 gtcgggctcc cggcgcccct gctcgcgcgg gtggatgtgc cggacgcgga gacgctggca
      841 ccactccatt acgactttcg gcggaaaggc ccgcccatca agcgagagga tcgctgggag
      901 gagctcacgc gcggggtcaa cctggcgctg ggtaagccct accgtttcga ccgccggccc
      961 aactatggcg tcacgcgcga tgaaggcgat gcccggcagc tcaccgacgg caagctcgtt
     1021 ccaggcgaca agatctggtt ctcgaaagag gcggtgggct accagggcgc cgacccgccg
     1081 gcgacgatct gcatcgacct cggtcaggtc tacccgatcg acgcggtggt ggcgcgcgtg
     1141 cagggaggtg gcgcctggga gggcagcttc cgctacccgc tgcagttcga cgtctacgtg
     1201 agtgacgatg accgcaccta tacccgcgtg gatagcgtgc gcaagcggga gtatcgcgac
     1261 cagcgcggca cactcttcga tcttcccgag agcgtgggcg ctcaaccccc cggcaatccc
     1321 cgcccccatc cattccactt tggccgcctc cgcacacgcg ggcgctatgt cgccttgcgc
     1381 atgacgcttg cgagcgccta tggtgccctg gacgagatcg ccgtcatgaa gggggaccac
     1441 gacccggcaa aggtcacctt cgatccgcag caggcgacca cgctcctctt tgacgccgtc
     1501 gagctgctct atccgcgtga ccggatgcgg gtgcccacga acgtcgcggg tggcttctcc
     1561 ttcgccgtgc gcgactcgcg gcgcgatacg cgccagccgg tcaccttcca tatcgatgtg
     1621 ccggccgctg ttaccctctc ctggtccggc tccgcatcgc tgcgttctca agagcacgcg
     1681 cgcgatggcc ggccgtatcg cgagtatacc ctcaccacgg taccatcggg cggcaagctc
     1741 t
//
LOCUS       DAOSCV010000968         1739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45885_length_1739_cov_2.282067, whole genome shotgun sequence.
ACCESSION   DAOSCV010000968 DAOSCV010000000
VERSION     DAOSCV010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45885_length_1739_cov_2.282067"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLY56_18160"
     CDS             <1..1178
                     /locus_tag="PLY56_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ23453.1"
                     /translation="IGSPQIWWLDGRPAPNCTSEDELLRTEGGFYWNKPRRQVWVHLP
                     AGKTLDDVEILIPVHGTCICINTDFVVVRDLRAIFSWNDGFDTHGTGKHIVFQRCIAT
                     DNCGQGFSVHDTNVALYEECLAERNASSGSCDVNECHSYYRRCVFVNNTFEAGVYGHD
                     QSQHTYEDCLILGNTPFEQVWQRGFSRMNFINCVIRGNSERSTGLISAAHGALHFREC
                     TLADAAYVCRLTDAETASLSITHSILARCKEPFLTLPNSKPRVILRDNIYHDGPGVQV
                     GDRVFGPEAWEDYQKSTGLDAGSVWRDPGLGGVFALQLLPGSPVLALRGQRPPQRIGA
                     VLPPSVWELYARTRNEVATPAGIVKRPAARSAVPAPLRMGSARGWEPHVVGLLGRST"
     gene            1178..>1739
                     /locus_tag="PLY56_18165"
     CDS             1178..>1739
                     /locus_tag="PLY56_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ23454.1"
                     /translation="MKIGVSSYSYIRLTRAGEMTLKETIAKAKEQGFDVIEILGLSSQ
                     APDCSDPEKLAEELREEAARCDIEIAAYTVAADLLRGCDGNFEKEVARIQREIDLAAI
                     MGAPCVRHDASAGFPPEHVGPKSFDAALPRLAEGCRILTEYAAAKGIKTTVENHGRFC
                     QDSRRVEKLVTAVNHPNFGVLIDIGNF"
BASE COUNT          300 a          565 c          582 g          292 t
ORIGIN      
        1 ggatcgggag cccgcagatc tggtggctgg atggccggcc ggcgccaaac tgcacgagcg
       61 aggatgagct tctgcgtacc gagggcggct tctactggaa caagccccgg cgccaggtgt
      121 gggtacacct gcctgccggc aagacgctcg atgacgtgga gatcctcatc ccggtgcatg
      181 gcacctgcat ctgcatcaac accgatttcg tggtggtccg tgacctgcgc gccatctttt
      241 cgtggaacga cggcttcgac acgcacggca cgggcaagca tatcgtcttc cagcggtgca
      301 ttgccacgga caactgcggc cagggttttt cggtccacga cacgaacgtg gcgctctacg
      361 aagagtgcct ggcggaacgg aacgcgagct ccggctcgtg cgacgtgaat gagtgccact
      421 cctactaccg ccggtgcgtc ttcgtgaaca acacttttga ggccggggtg tacggccacg
      481 accagtcgca gcacacctac gaggattgtc ttatcctggg caacacgccg ttcgagcagg
      541 tgtggcagcg cggcttctca cggatgaact tcatcaactg cgtcatccgc gggaactcgg
      601 agcgctctac cgggctcatc tccgcggcgc acggcgccct ccacttccgc gagtgcaccc
      661 tggccgacgc cgcctatgtc tgccgcctaa cggacgcaga gaccgcctca ctttcgatca
      721 cccactccat cctggcgcgg tgcaaggagc cgttcctgac gctgccgaac tccaagccgc
      781 gggtgatcct ccgcgacaac atctaccacg atggcccggg cgtgcaggtg ggcgaccgtg
      841 tcttcgggcc ggaggcgtgg gaggactacc agaagagcac ggggctggac gcgggctcgg
      901 tgtggcgcga cccgggcctg ggtggggtgt ttgccctcca gctgttgccg ggctcgccgg
      961 tgcttgccct ccgcgggcag cggccgcccc agcgcatcgg tgccgtgctc ccgccctccg
     1021 tgtgggagct gtatgcccga acccggaatg aggtggccac ccccgcgggg atcgtgaagc
     1081 ggccggcggc gcgctcggcc gtgccggcac cccttcggat gggatcggcg agaggatggg
     1141 agccccacgt cgttgggctg cttggaagga gcacctgatg aagattggtg tcagttctta
     1201 cagctacatc cgcctgacgc gggcgggaga gatgacgctg aaagagacga ttgccaaggc
     1261 gaaggagcag ggttttgatg tgatcgagat cctcgggctt agctcgcagg cgcccgattg
     1321 ctccgacccg gagaagctcg cggaggaact gcgtgaagag gccgcgcgct gcgatatcga
     1381 gatcgccgcc tacaccgtgg ccgccgatct cctccgcggg tgcgacggca acttcgagaa
     1441 ggaggtcgcg cgaatccagc gcgagattga tctcgccgcc atcatgggcg cgccgtgcgt
     1501 ccgccacgac gcgagtgccg gcttcccccc ggagcacgtg ggcccgaaga gctttgacgc
     1561 ggcactcccc cgcctggccg agggctgccg catcctcacc gaatacgccg cggctaaggg
     1621 gatcaagacg acggttgaaa accacgggcg cttctgccag gacagccggc gcgtggagaa
     1681 gctggttacc gcggtcaacc acccgaactt cggcgtgctg atcgacatcg gcaacttca
//
LOCUS       DAOSCV010000969         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_45985_length_1736_cov_5.223081, whole genome shotgun sequence.
ACCESSION   DAOSCV010000969 DAOSCV010000000
VERSION     DAOSCV010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45985_length_1736_cov_5.223081"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1736
                     /locus_tag="PLY56_18170"
     CDS             <1..>1736
                     /locus_tag="PLY56_18170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ23455.1"
                     /translation="GVLVEADGGNCSVTDPEGAYRLERLATGTYDLTLSPPEHSTFAR
                     QRITAVMVTAGETTTRDFVLRRAGEVKGRVTTAAGEPVSGAIVEVPEVQLAITDSDGY
                     YHMGGVPAGSQSLLVTPHEGANLLPARRDGVAVIAGQTAVADFTLDPTSMLSGTVKDS
                     TGTGVDGARIWVIPSERGNTGMLVTADSAGRYIVPNLAPGRYDVTATPPSGRPEILPK
                     TEHNLVISRGATQSLDFTLSEGGTITGVVRDHEGNPLSGTVYLSGVGPDATGAPVQGG
                     LLLTAEIGPDGRYTLVGVPAGTFILRPEAYSSEPLLSQQAHVTVAAGQVVEHDFILST
                     GGCVSGRIVGPSGDGVRALVRIIHPARGPERAFEALSGAAGNYRATAIPPGVNYTVVV
                     EPLDGSSLLRQVKTATVVDGQETVVDFQLAAGAVLTGRVTDGEGNPLGGVCVNFRGAD
                     TGRVYTDGEGTYQIKGLAEGVYDLYFAPPVDLPVVGQIVRGVTITAAASSVRDVQLAP
                     AGRIEGVVQSKTGEPVAGVAVQLHGSGTPIPRGPEEVPAVTPASLSQASETQWDRVIT
                     GADGRFVFTGLPA"
BASE COUNT          274 a          564 c          608 g          290 t
ORIGIN      
        1 ggcgtgctcg tggaggccga tgggggcaac tgctccgtca ccgatccgga aggcgcctat
       61 cgcctcgagc gtctggcgac cggcacgtac gacctcaccc tctcgccgcc ggagcatagc
      121 acctttgcgc ggcagcggat caccgctgtg atggtgaccg ctggagagac aaccacgcgg
      181 gacttcgtgc ttcgccgcgc tggcgaggta aagggccggg tgactaccgc cgcgggcgag
      241 ccggtgagtg gcgcgatcgt cgaggtgccc gaagtccaac tggcgattac cgatagcgac
      301 ggttactacc atatgggcgg cgtgcccgcg ggctcgcaga gtctcctcgt gacgcctcac
      361 gagggcgcga acctccttcc cgcgcgccgt gacggcgtgg cggtgatcgc cgggcagacg
      421 gcggtcgcgg acttcactct cgacccgacc tcgatgctga gtggaacggt caaagacagc
      481 accgggaccg gcgtggatgg cgcccggatt tgggtgattc cgtccgagcg cggcaatacc
      541 ggaatgctgg tgacggccga ctcggcgggt cggtacatcg tgcccaacct cgcccccggg
      601 cgctacgatg tgactgcgac tcctccgtca ggccggccgg agatcctccc caaaacggag
      661 cataacctcg tgatcagtag gggcgcaacg caaagcctcg atttcacctt gagtgaggga
      721 ggtacgatta cgggcgttgt gcgcgatcat gagggcaacc cgctttccgg aacggtgtac
      781 ctgagcggcg taggccctga tgcaaccgga gcgcccgtcc agggcgggct gttactcacg
      841 gccgagattg gcccggacgg ccgctatact ctcgtgggcg ttcctgccgg cacgttcatc
      901 cttcggccgg aggcctactc ctcggagccg ctgctgtcgc agcaggcgca cgtcacggtg
      961 gccgccggcc aagtggtgga gcacgacttc atcctctcga ctggcggctg cgtgagcggg
     1021 cgtatcgtgg gcccctcggg cgatggagtg cgggctctcg tccggatcat ccacccggct
     1081 cgcggtccag agcgcgcctt tgaggcgctg agcggtgctg cggggaacta ccgcgctacc
     1141 gccatcccac cgggcgtcaa ctacacggtg gtcgtcgagc cgctggatgg gtcgagcctc
     1201 ctccggcagg tgaagacggc gacggtcgtg gacggtcagg agacggtggt cgacttccag
     1261 ctggctgctg gcgccgtgct cacgggccgt gtcacggatg gcgaaggtaa ccccctcggg
     1321 ggcgtctgtg tcaacttccg cggtgcggac acggggcgcg tctataccga tggggaaggg
     1381 acctaccaga tcaagggtct cgccgagggc gtgtatgacc tctacttcgc gccaccagtc
     1441 gatctgccgg tcgtcggcca gatcgtgcgc ggggtgacta tcaccgccgc ggcatcgagc
     1501 gttcgcgatg ttcaactggc gcctgccgga cgcatcgagg gcgtcgtaca gagcaagacc
     1561 ggggagcccg tcgctggcgt ggctgtccag ctccacggat ctggaacgcc tataccgcgc
     1621 gggcccgaag aggtcccggc cgtaacgccc gcgagcctct cccaggcgag cgaaacccaa
     1681 tgggatcggg tgatcaccgg ggctgatggc cgatttgtct tcaccggcct gcccgc
//
LOCUS       DAOSCV010000970         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46152_length_1732_cov_3.074538, whole genome shotgun sequence.
ACCESSION   DAOSCV010000970 DAOSCV010000000
VERSION     DAOSCV010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46152_length_1732_cov_3.074538"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLY56_18175"
     CDS             <1..435
                     /locus_tag="PLY56_18175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ23456.1"
                     /translation="GDGPLTEPLRELARTLGIADRVVFAGLREDVPQLLRLMDLFALA
                     SLNEGMGRVLVEAGACRLPSVATRVSGIPDVIEEGVTGLLVDPASPEQMSEALMQLLE
                     DPERARRMGEAAYRKVVPAFGVEAMVERIEAVYQEVLAKKAR"
     gene            602..1324
                     /locus_tag="PLY56_18180"
     CDS             602..1324
                     /locus_tag="PLY56_18180"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ23457.1"
                     /translation="MVQEQFDDLLSRSRILVIAPHPDDETYGCGGLIARARAHGGQVY
                     VMIVSAGDLQHYDEQLSTVTASRRQKELEESMRVLGVEDYCILFTDRHSHMRMDSVPR
                     RDLLEEIERASPLAMDRLHPDMVILPAISYNQDHEAVFKAGFTACRPGMPTVRPVVKV
                     VLSCDAPQLGWNWEPFHPNVYVDISDYLEIKLKAHACHVSQARPAPHHGSLENLERLA
                     RLRGAEISVEAAEAFYCHRWIV"
     gene            complement(1536..>1732)
                     /locus_tag="PLY56_18185"
     CDS             complement(1536..>1732)
                     /locus_tag="PLY56_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23458.1"
                     /translation="PRRPAGTSCSADAREDGEKLSRLLAEGVALLRFADFCTRADFQP
                     VLGFGGLLLHDCCPMDEVVA"
BASE COUNT          327 a          538 c          562 g          305 t
ORIGIN      
        1 ggcgatggcc cgctcacgga gccgcttcgc gaactggcga gaacgctcgg catcgcggat
       61 cgcgtggtct tcgcgggcct ccgcgaggat gtgccgcagc tcctgcgcct gatggatctc
      121 tttgctctcg cctcgctcaa cgaggggatg ggccgcgtcc tggtcgaggc cggcgcttgc
      181 cgtctgccgt cggttgccac gcgcgtcagc ggcatcccgg acgtcatcga ggagggggtc
      241 accgggcttc tggtggatcc cgcgagcccc gagcagatgt cagaggcgct gatgcaactc
      301 ctggaggacc ccgagcgcgc ccggcgcatg ggagaagccg cctaccgcaa ggtcgtgccc
      361 gcattcggtg tcgaagccat ggtcgagcgg atcgaggcgg tctatcagga agtgctcgca
      421 aaaaaagcca ggtgatacga acgcccgtag agagcatcag gcgtgtcggt cggccgtgcc
      481 ctcccctggc aagaaaggct tgccggtgcc cggcggggag gggcctacat caggaaggaa
      541 cgatcttcgg gagccggcca ggcggccatt gccctggtcc atgaacaacc gcgaggaact
      601 gatggtacaa gaacaattcg atgatctcct ctctcgtagc cgcattttgg tgatcgcgcc
      661 gcaccccgat gacgagacct acggatgcgg cggcctcatc gcgcgtgcgc gcgcccatgg
      721 cgggcaggtg tatgtgatga tcgtctccgc tggcgacctc cagcactacg acgagcagct
      781 ctccaccgta actgcctcgc gccggcagaa agagctggag gagtcgatgc gcgtgctcgg
      841 cgtggaggac tactgcatcc tcttcaccga tcgccatagc catatgcgca tggactccgt
      901 gccccggcgc gaccttctgg aagagatcga acgcgccagc ccgctggcga tggatcgtct
      961 gcaccccgac atggtgattc tacctgccat ttcgtacaac caggatcacg aggcggtttt
     1021 caaagcaggt ttcacggcgt gccgaccagg aatgcccacg gtgcgcccgg tcgtgaaggt
     1081 ggtcctctcc tgcgatgccc cgcagctcgg ctggaactgg gagccgttcc acccgaacgt
     1141 gtatgtggat atcagcgatt acctggagat aaaattgaag gcccatgcct gtcatgtctc
     1201 ccaggcgcgg cccgcgcctc accatggcag cctggagaac ctggagcgct tggcccgcct
     1261 ccgtggcgcc gagatctcgg tcgaagccgc cgaagcgttc tactgccacc gctggatcgt
     1321 ctgaattgcg gatttcggat tgcggaatgg ccggttcgga agggcaggat ggagaaacac
     1381 gacctggagc agcgtacgtt ggcattcgct ctgcgcatca ttcgctttgt gaccagcctg
     1441 ccgaggaaca aggtcacgga tgtcgtcggc taccagctgc tcaagtcagg cacttccatt
     1501 gtggtgccaa ctaccgggag gcaaacagtg cagaatcacg cgacgacttc atccatagga
     1561 cagcaatcgt ggagaaggag gccgccgaaa ccgagtactg gttggaagtc tgcgcgggtg
     1621 cagaaatcgg cgaagcgcag gagcgccacg ccctccgcca ggagtcggga gagcttctcg
     1681 ccatcttcac gagcatcggc agaacaactc gtgcccgccg gtcgacgcgg ta
//
LOCUS       DAOSCV010000971         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46255_length_1729_cov_4.357228, whole genome shotgun sequence.
ACCESSION   DAOSCV010000971 DAOSCV010000000
VERSION     DAOSCV010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46255_length_1729_cov_4.357228"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>933)
                     /locus_tag="PLY56_18190"
     CDS             complement(<1..>933)
                     /locus_tag="PLY56_18190"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ23459.1"
                     /translation="EQGAYQSPGADMAYLKAYLLAKILWNPNCDVERHRREFLEGFYG
                     KAADQIDRYLRLCRAYVLEKGLHQNIWINPESGHMPSELVAQANALFDEAERAVADDP
                     DALYRVRVARLPIQYVQLTRVRPDFSKPWRIRGDSYGPEPDPAMVKVADQFFSICAQE
                     GVTHLSESRFSPEQLHERVAPAIYGAKLVTLENDALRLRVAPSLGGRILTIEEKATGQ
                     QLVSPAASSAAGFPYAGGSWDAAGFRRRTAGANASFRVDSPTTGNQVTLVATLKDGLE
                     LKRVIRLAGSGWTVETTVTNTADQPRAARIRSGL"
     assembly_gap    934..943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<944..>1729)
                     /locus_tag="PLY56_18195"
     CDS             complement(<944..>1729)
                     /locus_tag="PLY56_18195"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HOJ23460.1"
                     /translation="SSKVSRIPKKETITLGAIDDTQVPVLEYREPFWWDAFDGDWAAR
                     NKANSNQARLGEKHGGKITYVGFVHTFNRLVPPEKYFDEHPEYFSEIGGKRMKGYYQL
                     CLTNPDVLKIVIEGVRKWLRENPQASIVSVSQNDTRGYCECANCKKVEEEEGGAHSGP
                     LIRFVNAVAEAIEKEFPHVAIDTLAYQYTRKPCKTRPRHNVIVRLCSIECCFSHSLAE
                     EDTEFARDIEGWSKICNRLYIWDYTTTFAHYVLPFSNIEVLQPN"
BASE COUNT          266 a          545 c          589 g          319 t
ORIGIN      
        1 tcgagcccgg agcggatccg tgccgcgcgc ggctgatccg ctgtgttcgt caccgtcgtc
       61 tcaacggtcc agcccgatcc ggccaggcgg atcactcgct tgagctccag gccgtctttc
      121 agcgtggcca ccagcgtcac ctggttgccg gtggtcgggc tgtccacgcg gaagctggcg
      181 ttcgcgcccg ccgtccgccg gcggaatccc gcggcatccc acgagccgcc ggcgtagggg
      241 aagccggcag cgctggaagc ggccggcgag acgagctgct gcccggtggc tttctcctcg
      301 atggtgagga tccgcccgcc cagcgaaggc gcaacgcgca ggcgaagcgc gtcattctcc
      361 agggtaacga gcttcgcgcc gtagatagcg ggcgccacgc gctcgtgcag ctgctcaggg
      421 ctgaagcggc tctcgctcag gtgggtcacc ccctcctggg cgcagatgga gaagaactgg
      481 tcagccacct tcaccatcgc ggggtcgggc tcggggccgt aggaatcgcc gcggatccgc
      541 cacggcttcg agaaatcggg gcggacgcgg gtcagctgca cgtactggat cgggaggcgc
      601 gcgactcgca cccggtagag tgcatcgggg tcgtcggcga cggcgcgctc ggcctcatcg
      661 aagagggcat tcgcctgcgc gacgagctcg gagggcatgt gcccactctc ggggttgatc
      721 cagatgttct gatgtagtcc cttctccagg acgtaagcgc ggcacaggcg caggtagcgg
      781 tcgatctggt cggccgcctt gccatagaac ccctccagga actcgcggcg gtggcgctcc
      841 acgtcgcagt tggggttcca aaggattttc gccaggaggt aggccttcag gtaggccata
      901 tcggcgccgg gcgactggta ggcaccctgc tcgnnnnnnn nnngtttggc tggagcacct
      961 cgatgttgga gaaggggagc acgtagtgcg cgaaagtggt cgtgtaatcc cagatgtaga
     1021 gccggttgca gatcttcgac cacccctcga tgtcgcgggc gaactccgtg tcctcctctg
     1081 ccagggagtg cgagaagcag cattcgatgc tgcagaggcg cacgatgacg ttgtgccggg
     1141 gccgcgtctt gcacggcttc cgggtgtact ggtaggccag ggtgtcgatg gcgacgtggg
     1201 ggaactcctt ctcaatcgct tccgcgacgg cgttcacgaa gcggatgagc ggcccggagt
     1261 gggctccacc ttcctcctcc tcgaccttct tgcagttggc gcactcgcaa tagccgcgcg
     1321 tgtcgttctg cgagacagag acgatgctcg cctgcgggtt ctcgcgcagc cacttgcgca
     1381 cgccctcgat gacgatcttc aggacgtcgg ggttggtcag gcagagctgg tagtagccct
     1441 tcatccgctt gccgccgatc tcggagaagt actcggggtg ctcgtcgaag tacttctccg
     1501 ggggcacgag gcggttgaat gtgtgcacga agccgacata ggtgatcttc ccgccgtgct
     1561 tctccccgag ccgggcctgg ttggagttcg ccttgttccg cgcggcccaa tcgccgtcaa
     1621 acgcgtccca ccagaacggc tcgcgatact ccagaacggg cacctgcgtg tcgtcaatcg
     1681 cgcccagggt gatcgtctcc ttcttgggga tgcggctcac cttcgagga
//
LOCUS       DAOSCV010000972         1728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46305_length_1728_cov_3.524208, whole genome shotgun sequence.
ACCESSION   DAOSCV010000972 DAOSCV010000000
VERSION     DAOSCV010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46305_length_1728_cov_3.524208"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            483..1043
                     /locus_tag="PLY56_18200"
     CDS             483..1043
                     /locus_tag="PLY56_18200"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOJ23461.1"
                     /translation="MRVAQAEAPAANQATHSDTAEEKTLVLELAPTTDEATDALIMRL
                     VAEAREEEATKVSDSEASAKIKSKAVQQALKLRGSRYRYGGSSRGGFDCSGFTQYVYK
                     RMGVNLPHSSAAQFKCGEPVARKDLKPGDLVFFGRGSRYVGHVGIYIGDDKFVHASNP
                     SRGVVVDSLSSKYYASRYRGARRVRE"
     gene            complement(1339..>1728)
                     /locus_tag="PLY56_18205"
     CDS             complement(1339..>1728)
                     /locus_tag="PLY56_18205"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOJ23462.1"
                     /translation="LPAEEYEVVEYAILPDEVETVRVTLREWAARCDAVLTVGATGLG
                     PRDITPEATRSVLDRECPGIAEAIRARGYSKRPSAILSRGLAGTIGSCLVVNLRGSLE
                     GVEDGVEVLLPIMPQAVEVVRKLAQQH"
BASE COUNT          321 a          551 c          564 g          292 t
ORIGIN      
        1 gagtgggcgg gttctccttc atcgacctcg acgggcatct cctgacgggc gacacgcgca
       61 tggtaggggg cttcgtccag cagggcgggc ggctcgtgct ccagcctgac cagccggggc
      121 atggaacggc gcttgccaca acaaggggtt gacacccggg cggcgactcc ggtatactat
      181 aacaagttat tgacccggat gggccggatc cccaccgaaa cccgggcagg cacgcccgag
      241 gggggcataa gagacagttc aggcagtact gcggggccgg cgggtagagt tggtgccatc
      301 cttcaggaca gccagccact atcagctttc catcggtcat ccccgagcca tgccgctccg
      361 caaggaaggg acatgaggta ttgaccggga ccagtctgaa aagcacacgc attgtcgctg
      421 ccgcgacgtc actcttgttt gccgcatccg ctcttgcgca gagtgctccg gaacgcattt
      481 cggtgcgcgt tgcccaggcc gaggccccgg ccgccaatca ggcaacgcac tctgataccg
      541 ccgaggagaa gaccctcgtc ctggagttgg caccgaccac cgatgaggca accgatgcgt
      601 tgatcatgcg ccttgtcgcc gaggcccgcg aagaggaggc gacgaaggtc agcgatagcg
      661 aggcgagcgc caagatcaag agcaaggcgg tccaacaggc gctgaaactg cgtgggtcac
      721 gctatcgata cggcggttcc tcgcgtggcg gcttcgattg ctccggcttc acccagtatg
      781 tctacaagcg gatgggcgtg aatctgcccc actcctccgc agcccaattc aagtgcggcg
      841 agccggtcgc gcgcaaggac ctgaagccag gcgacctggt cttctttggc cggggcagtc
      901 gctatgttgg gcatgtgggg atctacatcg gcgacgacaa gtttgtgcat gcctcaaatc
      961 caagccgcgg cgtcgtcgtt gacagtctct cgtccaaata ctacgccagc cgctatcggg
     1021 gtgcgcgccg cgtgcgcgaa tgaatctgcg cgcgggacct ctccctccgg gacagcgccg
     1081 gagatgaacc cttagcgagg acgaacagtt ggtgggggcg aacctttagt gttcgccctg
     1141 ccacggaaat cgggcgaaca gcggaggcgc ggagctagaa gctccgcgct gcttgtgtgt
     1201 aaagccctac gggctaggta gggcttcatt cccgggtaac ccagggtagg gtgttgggag
     1261 ggggagccca aacggctttg ccgggtagta gcacggagct tccagccccg cgcccgggcg
     1321 tggtccacgc tcacaggctc agtgctgctg cgcgagcttg cgcactacct ccaccgcctg
     1381 cggcatgatc ggcagaagta cctccacacc atcttcgacg ccctccaggc tgccccggag
     1441 gttgacgacc aggcaggagc cgatggtgcc cgccaatccg cgagagagga tggcgctggg
     1501 gcgtttgcta tagccgcgcg cgcgaatcgc ctcggcgatg ccagggcatt cccggtcgag
     1561 cacactgcgc gtcgcttcag gcgtgatatc acgcgggcca agccctgtgg ccccgacggt
     1621 gagcacggca tcacaccgcg ccgcccactc gcgcagtgtg acccgcaccg tctccacctc
     1681 gtccgggaga atcgcgtatt cgacgacctc gtactcctca gcagggag
//
LOCUS       DAOSCV010000973         1727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46351_length_1727_cov_2.742225, whole genome shotgun sequence.
ACCESSION   DAOSCV010000973 DAOSCV010000000
VERSION     DAOSCV010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46351_length_1727_cov_2.742225"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PLY56_18210"
     CDS             complement(<1..1139)
                     /locus_tag="PLY56_18210"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOJ23463.1"
                     /translation="MYATLSRCRLRWLLIPLIAAVLAPMPCLASEDEDPSETEVVVTA
                     TRRRQAKQTASAAVTVITRAEIAASGAPTVAEVLRGAAGIDIPRQGPLGAAALPSLRG
                     SSAGQVLVLVDGRRLNSPQHGDVDLSDIAIENVERIEVVRGGASALYGSDAVGGVINI
                     ITRAPSGEPEVRLSQGYGSFNTQLLSGLYSGSTGRLRYALTAQRTSATNDFEYFDAQA
                     GREQTRNNAGYHSWDASVKLVADLGASRQLTFSAERSDAEKDLPGATSYPTPAAEQRD
                     QRTLLDLHYLTPLGEGGDITGRLYRVEQENRYLDPDSFPAPTDATHRTVMDVGEVLGH
                     SRLSGNQVLTYGIEARNQRLKSSMLGQHSRTTAAVFVQDQLELGR"
     regulatory      complement(1417..1553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          299 a          546 c          583 g          299 t
ORIGIN      
        1 aaacgcccca gctccaactg atcctgcacg aaaacggcgg cggttgtgcg ggagtgctgc
       61 ccgagcatac tgctcttcag gcgctggttg cgggcctcga tcccataggt gaggacctga
      121 ttgcccgaga ggcgcgagtg cccgaggact tcgccgacat ccatcaccgt gcggtgggtg
      181 gcatcggtag gtgcagggaa cgaatcggga tcgaggtaac gattctcctg ctcgacgcgg
      241 tagagccgcc cggtgatgtc gccgccctcg cccagggggg tcaggtagtg gagatcaagg
      301 agtgttcgct gatccctctg ctctgcggcg ggcgtaggat acgaggtagc gcccggcagg
      361 tccttctccg cgtcgctcct ctcggcagag aaggtgagct ggcggctcgc gcccagatcg
      421 gcgaccagct tcacgctggc atcccatgag tggtagccgg cgttgttccg ggtctgctcg
      481 cggcccgcct gggcgtcgaa gtactcaaag tcgttggtcg cggaggtgcg ctgtgctgtg
      541 agggcgtagc gcaggcggcc ggtgctcccg gagtagagcc ccgagagcag ctgcgtgttg
      601 aaggagccat atccctgcga aagccggacc tccggctcgc ccgaaggcgc tcgtgtgatg
      661 atgttgatca cgccgccgac ggcgtcggag ccatacagcg ccgatgcacc gccacgcacc
      721 acctcgatcc gctccacgtt ctcgatagcg atatcggaga ggtctacatc cccgtgttga
      781 gggctgttga ggcggcggcc atcaaccagg acgagcacct gaccggcgct cgaaccgcgc
      841 aggctgggca gcgccgcggc gcccagcggg ccctggcgcg ggatgtcgat gccggccgcg
      901 ccacgaagca cctcggcgac cgtaggagcg cccgaggccg caatctcggc acgtgtgatg
      961 acggtgacgg cggcggacgc ggtctgcttt gcctggcgcc gtcgggtggc tgtcaccacc
     1021 acctcggtct cggagggatc ttcgtcttcg ctggccaggc agggcatggg agccagcacc
     1081 gccgcgatca gcgggattag cagccagcga agccggcagc gggagagtgt ggcatacatt
     1141 cacgaactcc tctcactacg atggggcatc cacgtgccgg tcacggtccc aacgtgcgcg
     1201 cgggagcccc gagccaatcg cttggaggca cttgtgacgc tgcggtcaac aaaaaacccc
     1261 accttcggta tgaagatgag gccggttgct ccgaatgctg atcacgcatc gcgcaccgca
     1321 ggggtgatgg gcgagccgcg ggcacaatcc agaatccatc ttccagatag cccgatcgag
     1381 tgatccggag tgtcaccagc gcctttctcg cgaagggctg aatgaccagt tacaggctgg
     1441 gtgtctgact cgggcgtgcg cccacacagt agcgggactg tgccggaatc tcaccggctt
     1501 ccccatttca cccggcgtcc gcgaacgccg ggacctgcac cgtcagtatg cggttgtctg
     1561 gcgtattata atggccattc gcacgcttgt caagggcttc cacgagaagg gctgcgtttc
     1621 tggcggctgc cgtgatctct tacgagagga aacccccgtc gcgcgcggaa gcttgtgacc
     1681 aggcaaatcg cgagcgactc ccgagaatgg cgcgctctgg acctccc
//
LOCUS       DAOSCV010000974         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46468_length_1724_cov_2.540443, whole genome shotgun sequence.
ACCESSION   DAOSCV010000974 DAOSCV010000000
VERSION     DAOSCV010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46468_length_1724_cov_2.540443"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="PLY56_18215"
     CDS             complement(<1..1637)
                     /locus_tag="PLY56_18215"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ23464.1"
                     /translation="MPAQTTPEQAASSTADFLYFLAGDAQLDPQGSAHLALIRDLLEP
                     FAAGRTNQALTVICQRVAALMGVPRCSLCLWDEPSGCLVLAAQAGPSGALFPAQPAFP
                     TSPECLPDWVREMERLGSSMAPPRPSRLQRRRRRARSFPDTTSPPWDAYPVLGIPLSA
                     AGRAIGFLFCVGIGESSAFTERHIRLGNILAATLAPAIGAAWANRRDPPVDTADVRSA
                     RLALSGSLDPDEVLQIVTDMARERLGAQVCVALSFQDAHSFRVCVAAEWENAEVGIDL
                     TGDGLASQALRLGRPVWIPDRVRPPRILPREIATRVPLRAGIAVPLREGDQVTGAILA
                     YHSLRRDYSAEEVRLLASLAAQAGAALRNCVLYERQRLVAETVQRALAPRLPPIVEGV
                     DVGHLYLPKADQIGGDHYDLIPLGGGRLGIAMGDVAGSGLRAAIHTTMARYMARAFAF
                     ETPRPGTILQKLNEAICTEGHSEVFLTLFCGVLHSSGRLHYANGAHPYPILRRAGRSA
                     PLFLETTGTVVGVIPDQHYDEREVRLHSGDILLLYTDG"
BASE COUNT          299 a          557 c          618 g          250 t
ORIGIN      
        1 ccgtcggtgt agaggagcag gatatcgccg gagtggagac gtacctcacg ctcgtcgtag
       61 tgctgatcgg ggatgacccc cacgacggtg cccgtcgttt ccagaaaaag gggcgcgctc
      121 cggccggcgc ggcgcaggat cggatagggg tgcgcgccgt tggcatagtg cagccgtcca
      181 gaagagtgca acaccccgca gaaaagcgtg aggaagacct cggagtggcc ctcggtgcag
      241 atcgcctcat tcagcttctg aaggatcgtt ccggggcgag gcgtctcaaa cgcgaaggca
      301 cgcgccatat agcgcgccat cgtggtgtga atggctgcgc gcaggccgct gccggccacg
      361 tcacccatcg cgatcccgag acgcccgccg cccagcggga tgaggtcgta gtgatcccca
      421 ccgatctggt cggctttcgg gaggtagaga tgccccacat ccaccccctc gacgatgggc
      481 ggcagcctgg gagcgagagc gcgctgcacc gtttcggcca cgaggcgctg gcgctcgtag
      541 aggacgcagt tgcgcaaagc ggccccggct tgcgcggcaa gcgaggcgag caagcgcacc
      601 tcctccgccg agtagtcgcg gcgcagcgag tggtatgcca ggatggctcc agtcacctgg
      661 tcgccttccc ggagcggcac cgcgatgccg gctcgcagtg gaacgcgcgt ggcaatctcg
      721 cgggggagaa tacggggcgg gcgcacgcgg tccggtatcc agaccggccg gcccaggcgg
      781 agtgcctggg atgccaggcc atcgccggtg agatcgatgc caacttcggc attctcccac
      841 tcggccgcga cgcacacccg gaaggaatgt gcatcctgga aggagagagc aacacacacc
      901 tgagcgccca gccgctctcg ggccatgtcg gtgactatct gaagcacctc gtctggatcg
      961 aggctgccgc tcaaagcgag gcgtgcgctt cttacgtccg cagtgtccac cggcggatcg
     1021 cggcggtttg cccaggcggc gccgatggcg ggtgccaggg tggccgccag aatgttgccc
     1081 aggcggatgt ggcgttccgt gaaggctgac gactcgccta tgcctacaca gaagaggaac
     1141 ccgatcgccc tgccggctgc cgagaggggg atgccaagca ccggataggc atcccagggc
     1201 gggctcgtcg tatccgggaa cgagcgggcg cgccggcggc ggcgctgcaa gcgcgatggc
     1261 cgaggtggcg ccatgctaga gccgagacgc tccatctcgc gcacccagtc gggaaggcac
     1321 tccggagagg tggggaacgc tggctgtgcc gggaagaggg cgccggacgg cccggcctgg
     1381 gccgcgagca cgaggcatcc gctgggctcg tcccagagac agagcgagca tcgcggcacg
     1441 cccatcaaag cggccacacg ctggcagatc accgtgagcg cctggtttgt tcttcctgcc
     1501 gcgaagggct ccaggaggtc gcggatcagg gcaaggtgcg ccgagccctg cgggtccagc
     1561 tgggcatctc cagccagaaa gtacaggaag tccgccgtgg aagacgcagc ttgctcgggt
     1621 gtcgtctggg caggcatctg agcccgcgac gacgctgcgg ccgggagcct gccttcttcg
     1681 cgtctgccgg ggagctcgcc catcgatcca gtctcgcctt cccg
//
LOCUS       DAOSCV010000975         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46668_length_1718_cov_3.260974, whole genome shotgun sequence.
ACCESSION   DAOSCV010000975 DAOSCV010000000
VERSION     DAOSCV010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46668_length_1718_cov_3.260974"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLY56_18220"
     CDS             <1..725
                     /locus_tag="PLY56_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23465.1"
                     /translation="RLERRPHPKNRRDMMKALTLPEQDVLMRIIVSIDNFCATRSTTW
                     EAASALLQYLACFGVDAVRSALAAGERYGAFRMAKYPHPADQDRPPTTGISLNREVPF
                     VARVLERRNTLLRALYAAHERCAGTWEQDIAAITGEKAALWRSILTHERLIRPAESGA
                     NNGNRYVLSRSHVVVRAACGLPPAEASESAQEGPEPVGPFSPLAHTVAVEDVPARHPD
                     GNGRKKMEPAIDWSTVGPGVFD"
     gene            824..>1718
                     /locus_tag="PLY56_18225"
     CDS             824..>1718
                     /locus_tag="PLY56_18225"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOJ23466.1"
                     /translation="MQAGIRDTIAQQIGHTLARTDFDWLGRKYEGKVRDNYSAGDRRF
                     IIVTDRLSAFDRVIAPIPFKGQVLNEMAAFWFERTGSIVKNHMLAMPDPNAMVVRECQ
                     PLPIEVVVRAYLTGVTSTSIWTQYQQGARVFCGHKLADGMVKNQPLPEPIVTPSTKAE
                     KGGHDESVSAEEIVRRGLLSPELMEQVSEVSLALFRFGSELLAKHNLILVDTKYEFGL
                     CNGELVLIDEIHTPDSSRFWIRDTYEERFARGEEPEKIDKEYLREWLASQGFRGDGEI
                     PEIPTEVIVETAYRYLSAAERI"
BASE COUNT          317 a          563 c          550 g          288 t
ORIGIN      
        1 tgcggctcga gcgacggcca cacccgaaaa accgccgcga tatgatgaag gcgctcaccc
       61 tgccggagca agacgtgctg atgcggatca tcgtgagcat cgacaacttc tgcgccacgc
      121 gcagcaccac ctgggaggcg gccagcgcct tgcttcagta tctggcgtgc tttggagtgg
      181 atgcggttcg tagcgcgctc gcggccggcg agaggtatgg cgcctttcga atggcgaagt
      241 atccccatcc cgccgatcag gatcgcccgc cgaccaccgg gatctccctg aaccgggagg
      301 tgcccttcgt cgcacgcgtg ctggagaggc ggaacacgct cctccgcgcg ctgtacgcgg
      361 cgcacgagcg atgcgccggc acctgggagc aggatatcgc cgccatcacc ggcgagaagg
      421 ccgccctgtg gcgctctatc ctcactcacg agcgcctcat ccgtcccgct gagagcggcg
      481 caaacaacgg gaatcgctat gtgctctctc gctcgcacgt ggtcgtgcgc gcggcctgcg
      541 ggctgcctcc cgcggaagcc tccgagagtg cccaggaggg gcccgagccg gttgggccgt
      601 tctcgcccct cgcgcacacg gtggcggtgg aggacgtgcc ggcacgccac cccgatggca
      661 atggccgaaa gaagatggag ccagccatcg attggagcac ggtgggccct ggtgtcttcg
      721 actgagcgcc gctccatact atgcgccggg gcggagatcc acccgtggat ctccgcccca
      781 gcaaacgatt cttagtacca gccctgagag ggagcagcac aaaatgcagg caggtattcg
      841 cgacacgatt gcacagcaga ttggccacac gctggctcgc accgattttg attggcttgg
      901 ccgcaagtat gaaggaaagg tgcgtgacaa ctacagcgcc ggcgaccgcc gtttcatcat
      961 tgtcaccgat cgcttgagcg cctttgaccg cgtcatcgcc cccatcccgt tcaaggggca
     1021 ggtgctcaac gaaatggcgg cgttttggtt cgagcgcacc ggctccatcg tcaagaacca
     1081 catgctggcg atgcccgatc cgaacgccat ggtcgtgaga gagtgccagc cactccccat
     1141 cgaagtggta gtccgcgcct atctcaccgg cgtgaccagc acctcgatct ggacgcagta
     1201 tcagcagggc gcgcgcgtct tctgcgggca caagctagcc gacgggatgg tgaagaacca
     1261 gccgcttccc gagccgatcg tgacgccgtc caccaaggcg gaaaagggcg ggcatgacga
     1321 gtcggtctcc gccgaggaga ttgtccggcg cggcctcctc tcgcccgagt tgatggagca
     1381 ggtctctgag gtgagcctgg cgctcttccg cttcggctcg gagctgctgg cgaagcacaa
     1441 cctgatcctg gtcgatacga agtacgagtt cgggctctgc aatggcgagc tggtgctcat
     1501 cgatgagatt catacgcccg actcctcgcg cttctggatc cgcgatacct acgaggagcg
     1561 cttcgcccgc ggcgaggagc cggagaagat cgacaaggag tacctgcgcg agtggcttgc
     1621 cagccagggc ttccggggcg acggcgagat ccccgagatc cccaccgagg tgatcgtcga
     1681 aaccgcctac cgctatctct ccgccgccga gcggatca
//
LOCUS       DAOSCV010000976         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46794_length_1715_cov_3.206627, whole genome shotgun sequence.
ACCESSION   DAOSCV010000976 DAOSCV010000000
VERSION     DAOSCV010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46794_length_1715_cov_3.206627"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..>1715
                     /locus_tag="PLY56_18230"
     CDS             55..>1715
                     /locus_tag="PLY56_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ23467.1"
                     /translation="MSATRDGVRLTWPRVQGAEAYRIYRAAVTDARTAGSYQQIIEVP
                     ATQAQQEQYEDKPPIVAGVRYAYQVAGVAGGKVGSRMNGDRAMGPGEPEPVRDVQVSR
                     NLRSVQLTWPVATQEFVTAYRVYRSAQPNEGYERVTEVPASANTYTDSGLPGGATYFY
                     KVAAVSETGLESNWQSMQPVAAQTDLRPSAPAGVTATAPEGERRIIVSWRANTEPDIL
                     HYQVYRSAQRSRAGAPAAYGNLWTKFPKGKIGRGGPWQLLPGADKIPANVTSVEDNSV
                     DFNTTYYYVVRAVSAQGESPYSDEASATPNRRPGVVQNVTAKAGDGRVTITWGPLAEV
                     DIAGYHVYRQVSEQPTTRTRLTDQPVTGLQYVDTQVTNDTTYQYFVTAIDTAGWEGQL
                     SLPAEAMPTAPPEAPRNVLASAGNNLVTLVWDKNSESNLLEYRVYRSDSPNLTPQLHG
                     RVPVTSVDPPSIVFQDKAAQNLVTYYYQVTAVNTSGVESARSPSGMPVSATPAIPPAA
                     PTNVQAVGGDKSVVITWSPVTRLAPPDNRELGSVGHVLRSYRIYRT"
BASE COUNT          301 a          532 c          618 g          264 t
ORIGIN      
        1 tcgcttctgg gtggcgatga tgatggacca tctgcgccgg gtgcaggcgt cctggtgagc
       61 gcgacgcgcg acggggttcg cctgacgtgg ccgcgggttc agggggccga ggcgtatcga
      121 atctatcggg cagccgtgac agacgcccgc acagctggct cgtaccagca gatcatcgag
      181 gtgccggcta cccaggcgca gcaggagcag tacgaggaca agccgcccat cgtcgcaggc
      241 gtgcgctatg cctatcaggt ggctggcgtg gccggaggca aagtagggtc gcgaatgaat
      301 ggcgaccggg cgatgggccc cggtgagccg gagccggtgc gggatgtgca ggtgagtagg
      361 aacctgcgca gcgtgcagct gacgtggccg gtggcgaccc aagagttcgt gacggcgtat
      421 cgcgtttacc ggtcggccca gccgaatgag ggctatgagc gggtgaccga ggtgcccgcg
      481 tcagcgaata cctacacgga ttctggacta cccggcggtg ctacctactt ctacaaggtg
      541 gcggcggtga gcgagaccgg gctggagagc aactggcaga gcatgcagcc cgtggccgcc
      601 cagacggatc tgcggccctc ggccccggcg ggcgtgacgg cgacggcccc tgagggcgag
      661 cgccggatca tcgtctcctg gcgggcgaac acggagccgg acatccttca ttatcaggtt
      721 taccggagtg cgcagcgctc gcgcgctggc gcgccggcgg cctatggcaa cctgtggacc
      781 aagttcccca aggggaagat cggccgcggc ggcccgtggc agcttctgcc cggcgccgat
      841 aagatcccgg cgaacgtgac ctcggtcgag gataacagcg tcgacttcaa cacgacgtat
      901 tactacgtgg tccgtgcggt gagtgcgcag ggcgagagcc cgtattcgga tgaggcttct
      961 gccacgccga accggcgccc tggcgtggtg cagaacgtca ccgcgaaggc gggagacggt
     1021 cgcgtgacaa tcacgtgggg cccgctggct gaggtcgata tcgccggcta ccatgtgtat
     1081 cgccaggtgt cggagcagcc gacgacgcgc acgcgcctca cggatcaacc cgtgactggc
     1141 cttcagtacg tggacacgca ggtcacgaac gatacgactt accagtactt cgtgaccgcg
     1201 atcgacacgg ccggctggga ggggcagctg tcgctacccg ccgaggcgat gccgacggcg
     1261 ccgcccgagg ccccgcgcaa cgtgctggcg tccgccggca acaacctggt gacgctggtc
     1321 tgggataaga atagcgagtc gaacctcctg gagtaccgcg tttaccgcag cgactcgccg
     1381 aatctgacgc cgcagctgca tggccgggtg cccgtcacga gcgtggaccc gcccagcatc
     1441 gtcttccagg ataaggcagc gcagaacctg gtgacgtact actaccaggt gacggctgtg
     1501 aacacgtccg gtgtggagag cgcgcgtagc ccgtccggga tgccggtctc ggcgacgccc
     1561 gctatcccgc ccgcggcgcc aacgaacgtt caggcggtgg gtggcgataa gagcgtggtg
     1621 atcacgtgga gcccggtgac gcgcctggct ccgccggaca atcgtgagtt gggcagcgtc
     1681 gggcacgtgc tgcggagcta ccgcatctat cgcac
//
LOCUS       DAOSCV010000977         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46930_length_1712_cov_2.075438, whole genome shotgun sequence.
ACCESSION   DAOSCV010000977 DAOSCV010000000
VERSION     DAOSCV010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46930_length_1712_cov_2.075438"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1712)
                     /locus_tag="PLY56_18235"
     CDS             complement(<1..>1712)
                     /locus_tag="PLY56_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ23468.1"
                     /translation="WGVANSVSHVETHGEATTHGQADTTGEADTSGTSVAHSRVVTSM
                     HAETESEGYARSQSQSWGRGHIESEVLGSGRSVMGGETLGAGLTLGVAPGISVNRTYQ
                     VEDDLADRLTEVMRGFEGLLNAASHGGGFITSAYLITESEPGAQAAAALVSEAFYGDS
                     TPTSVMTVVPAEDEQEAVMEAAWALVPYAQPAGDPADPFDGWLFWKGATLLPADKVAG
                     YVAPAVLREGTLKVIAPIPEGMGFYPQMPGEAVLGHQYSPETGDLTTAPVRLDKPRLM
                     HTLFAGDTGWGKSVGTMRMVYEMAVKWGMRVVVLDFGFAWRMLLNAPGLEGRVDIRQL
                     RPDGVRPLRWNPLQIGRYINPETQLKAFVDIFGAVAQLGQKQQQHRLLDALRQVYVKA
                     GVMVDDPEVRASPQWGRVTALEAHALGLAADTPLGNLSADERQRLAVHRSRAVGLDDL
                     YREIERQYEALPPRDQVGRSVLEGILWRLKSLVRGAPAAQFAAGPDAEVVEDLGRPNG
                     VVVLEGGKFLDNFAKAWLLGWAGWLIYSDMVARRERQINRGEADLFMVFEEANIIFTG
                     LDGG"
BASE COUNT          264 a          685 c          480 g          283 t
ORIGIN      
        1 gccgccgtcc agcccggtga agatgatgtt cgcctcttcg aacaccatga acaaatccgc
       61 ttcgccgcgg ttgatctgcc gctcccgccg cgccaccatg tccgagtaga tcagccaccc
      121 tgcccagccc aggagccacg ccttggcaaa gttgtccagg aacttcccgc cttccagcac
      181 caccacgccg ttgggtcgcc ccaggtcttc caccacctcg gcatccggcc cggcggcaaa
      241 ctgcgccgcc ggcgcgccgc gcaccaaact cttcaagcgc cacaggatgc cttccagcac
      301 gctccgcccc acctggtcgc gcggcggcag cgcttcatac tgccgctcga tctcccgata
      361 cagatcgtcc aggcctaccg cgcgactgcg gtgcaccgcc agacgttggc gctcgtccgc
      421 gctcaaattg cccaagggcg tgtccgccgc caagcccaag gcgtgcgctt ccagcgccgt
      481 gacgcgtccc cactgcggag aggcgcgcac ttcgggatcg tccaccatca cccccgcctt
      541 gacatacacc tgcctcaggg catccagcag gcggtgttgc tgttgcttct gccccaactg
      601 cgctaccgcc ccgaagatgt ccacaaaggc tttgagttgc gtctccgggt tgatgtagcg
      661 cccgatctgc aagggattcc agcgcaaggg tcggacgcca tcgggacgca gttggcggat
      721 gtccacccgc ccctccaggc ccggcgcgtt caaaagcatg cgccaggcaa agccaaaatc
      781 cagcaccacc acccgcatcc cccacttcac cgccatctca tacaccatcc gcatcgtccc
      841 cacgctcttg ccccacccgg tatcgcccgc aaagagcgtg tgcatcagcc gcggcttgtc
      901 cagccgcacc ggagccgtgg ttaggtcgcc cgtctccggg ctgtattggt gccccagcac
      961 cgcctcgccc ggcatctgcg ggtaaaagcc catcccctcc gggatcggcg cgatcacctt
     1021 cagcgtcccc tcgcgcaaga ccgccggcgc gacatacccc gccaccttgt cggccgggag
     1081 cagggtcgcg cccttccaga acagccatcc gtcaaaggga tcggcagggt cgcccgcggg
     1141 ttgggcatac ggcaccagcg cccacgccgc ctccatcacc gcctcttgct cgtcctccgc
     1201 cggtaccacc gtcatcaccg acgtcggcgt ggaatcgccg tagaacgcct cgctcaccag
     1261 cgccgccgcg gcctgcgcgc cgggttcgct ctccgtgatc aggtaggcgc tggtgatgaa
     1321 gcccccgccg tggctggcgg cgttgagcag accctcaaac ccgcgcatca cctccgtcag
     1381 ccgatccgcc aggtcgtcct ccacctggta ggtgcggttg acgctgatcc ctggcgctac
     1441 gcccagggtc agccccgcgc ccagcgtctc gccgcccatc acgctccgac cgctacccag
     1501 cacctcgctc tcgatatgcc cgcgccccca ggactgcgac tgactgcgcg cgtagccctc
     1561 gctctcggtc tcggcgtgca tgctggttac cacgcggctg tgggctacgc tcgtcccgct
     1621 ggtgtccgcc tcgccggtgg tgtcggcctg gccgtgcgtg gtcgcctcgc cgtgggtctc
     1681 aacgtggctg acggaattgg cgacacccca ac
//
LOCUS       DAOSCV010000978         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_46979_length_1710_cov_4.051360, whole genome shotgun sequence.
ACCESSION   DAOSCV010000978 DAOSCV010000000
VERSION     DAOSCV010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46979_length_1710_cov_4.051360"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLY56_18240"
     CDS             complement(<1..270)
                     /locus_tag="PLY56_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ23469.1"
                     /translation="MNSALAITLLLLAGAAGVGPAAGETGKPPVVFHVATNGNDSWSG
                     RLPEPNRAKTDGPFATITRARDAIRQLKVGGGLNAPVEVRLRGGVY"
     gene            494..1501
                     /locus_tag="PLY56_18245"
     CDS             494..1501
                     /locus_tag="PLY56_18245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ23470.1"
                     /translation="MTHRERMYAALRHQQPDRCPYVISLTQKAHAAFVAHYGSDELLK
                     RIDNSVAGVAAQPGPKERWLSDTVWQDEFGVQWDRSIDRDIGNVCNCVLPERDLSRLE
                     LPDPNDPAKFEGFADAVAAARKQGKFVQFGIGFSLFERAWTLRGMEALFYDMIEAPSF
                     VDELLDAICDYNVALVRRAVTYDIDAIHFGDDWGSQRGLLMGRKLWERFLKPRLARMY
                     AAGKEAGKFVTIHSCGEVQELFPQLIEIGLDCFNPFQPEVMDPYEMKRLYAGKLSFWG
                     GVSTQRLLPHGTPDEVRAEVRRLVAEVGKEGGYVLAPAHCIPGDARPENILALIDAAN
                     E"
BASE COUNT          271 a          613 c          553 g          273 t
ORIGIN      
        1 atacaccccg ccccgaaggc ggacttccac cggcgcgttg aggccgcctc ctaccttcag
       61 ctgccggatc gcgtcgcgcg cccgcgtgat cgtggcgaat gggccgtccg tcttcgcccg
      121 gttcggctcc ggcagccgcc cgctccagga atcgttgcca tttgtcgcca cgtggaagac
      181 caccggcggc ttccccgtct cgccggcggc cggccccacc ccggcggctc ccgccagcag
      241 cagcagagtg atcgcgagcg cagagttcat ggcactgctc ctctcaccca ggcgatccag
      301 tgaccaccgc gcccgtggcc gcgccaccac gagagtcatc tcccggcgcg gccgccgtcg
      361 cgcgcgttac tgggccattc aacggcgcgc ggcccggttc ctggcacgcc tggaaactga
      421 gccgcgggga ggaagcgggc ggtgcttggc gaacccatct attgccgccg tacctacgaa
      481 ggagaacaga tcgatgaccc accgggaacg gatgtacgcc gccctgcggc atcagcagcc
      541 cgaccgctgc ccctacgtga tcagcctcac ccagaaggcc catgccgcgt tcgtcgccca
      601 ctacggcagc gacgagctcc tcaagcggat tgataactcg gtcgctggcg tcgccgccca
      661 gcccgggccc aaggagcggt ggctctcgga taccgtctgg caggacgagt tcggcgtcca
      721 gtgggaccgc agcatcgacc gcgatatcgg caacgtctgc aactgcgtcc tccccgaacg
      781 cgatctcagc cgccttgaat tgccggatcc gaacgacccc gcgaagttcg aggggttcgc
      841 ggacgccgtc gccgccgcac gcaagcaggg gaagttcgtc cagttcggca tcggcttctc
      901 gctcttcgag cgggcgtgga cattgcgcgg catggaggcg ctcttctacg acatgatcga
      961 ggcgccctcc ttcgtcgacg agctcctgga tgccatctgt gactacaacg tggccctcgt
     1021 ccgccgcgcc gtaacctacg acatcgacgc gatccacttc ggcgacgact ggggctcgca
     1081 gcgtggcctg ctgatgggcc ggaagctctg ggagcgcttc ctgaagcccc ggctggcgcg
     1141 catgtacgcc gcgggtaagg aggccggcaa gttcgtcacg atccactcct gcggcgaggt
     1201 gcaggagctc ttcccgcagc tcatcgagat cgggctggac tgcttcaacc ccttccagcc
     1261 agaggtaatg gacccctacg agatgaagcg gctctatgcc gggaagctca gtttctgggg
     1321 cggagtgagc acccagcgcc tgctccccca cggcacgccg gacgaggtcc gagccgaggt
     1381 gcgccggctc gtcgccgaag tcggcaagga gggcggctac gtcctggccc ccgcccactg
     1441 catccctgga gacgcccggc cggaaaacat cctggcgctg atcgacgccg ccaacgagtg
     1501 atcgtcctgc ggcgcggtga gctcttccct caccggcggc atcgacctcc gcgctgcttg
     1561 tgagcgaagc cctgccgggc tgagtcgggc tacactcccg ggtgttttag aacggcgcag
     1621 gctgggatgg gaagccgtcg cgcggggatc aatccccgcg ctgcttgtga gcgaagccct
     1681 gccgggctga gtcgggctac actcccgggt
//
LOCUS       DAOSCV010000979         1709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47031_length_1709_cov_2.903265, whole genome shotgun sequence.
ACCESSION   DAOSCV010000979 DAOSCV010000000
VERSION     DAOSCV010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47031_length_1709_cov_2.903265"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..899
                     /locus_tag="PLY56_18250"
     CDS             42..899
                     /locus_tag="PLY56_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ23471.1"
                     /translation="MDAKTLAVLGVTLLSWSSAFAGIGAGLRAYSPGHLVLFRFLVAA
                     SALLLYAVPARIPLPRGRDLPGLFLVGFLGFTIYHIALVYGQVTVDAGTTALLIATSP
                     ALTALVARILLRERLKPLGWAGIAISFTGAALIAFGKSGGMRFAPGALLILLSAFTTS
                     LFNVLQKPYLRRYRALDVATYALCLGTLPMLVFLPGLATAIATAPLSATLAIIYLGLF
                     PLAIGYAGWSYALSRAPAAIVSSFLYLSTPLALVIAWVWLGQVPAPLSLVGGAIALAG
                     VILVNTRGR"
     gene            complement(912..1451)
                     /locus_tag="PLY56_18255"
     CDS             complement(912..1451)
                     /locus_tag="PLY56_18255"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOJ23472.1"
                     /translation="MSLILGVSGSPRRDGNTDLLVREGLRLLEERAGASTEFVRVADL
                     RIERCRGCRACMRLGRCAIQGDDFEDLMARFFQADLYLLGAPIYWLGPPGCMKDFIDR
                     THGYYTDHSILRGKRAALITVGADSGFEPHEAVMESWLRVYGAHVVGKARVFAREKGE
                     VLEQPQAFESVRRLVEDLC"
BASE COUNT          253 a          646 c          490 g          320 t
ORIGIN      
        1 tcgcgatcct ggtcaccgtc ctctcgctca ttcagggccg gctggacgcg aagactctcg
       61 cggtgctggg ggtcaccctg ctctcgtggt cctcggcgtt cgccggcatc ggcgcagggt
      121 tgcgggccta ttcgcccggg catctggtcc tcttccgctt cctcgtcgcc gccagcgcgc
      181 tgctcctcta cgccgtgccg gcgcgtattc ccctgcccag aggccgagac ctacccgggc
      241 tcttccttgt cggattcctg ggcttcacca tctatcatat cgccctcgtc tacgggcagg
      301 tgacggtgga cgccggcacc accgcgctcc tgatcgccac ctcacccgcg ctcactgcgc
      361 tcgtcgccag gatcctcctg cgcgagcgcc tgaagccgct ggggtgggcg ggcatcgcga
      421 tcagcttcac gggcgcggcg ctcatcgcgt tcggaaaaag cggcggaatg cgcttcgcgc
      481 cgggagcact cctcatcctc ctctccgcct tcacaacgag cctcttcaac gtcctccaga
      541 agccgtatct gaggcgctac cgggccctcg atgtcgctac ctacgccctc tgtctgggca
      601 ccctgccgat gctggtcttc ctgccaggcc ttgcgacggc catagccacg gcgccgctca
      661 gtgccacgct ggcgatcatt tacctgggtc tcttccccct ggcaatcggt tacgccggct
      721 ggagctacgc gctctcgcgc gccccggccg ccatcgtctc cagcttcctc tacctctcaa
      781 cacctctggc gctcgtcatc gcctgggttt ggctgggcca agtgccggcg cccctctccc
      841 tggtcggcgg cgccatcgct ctcgccggag tcatcctggt caacacgcgc ggccggtagc
      901 gcggcctgtc ctcagcagag atcttccacc agccggcgca cgctctcaaa cgcctggggc
      961 tgctcgagca cctctccctt ctctcgggcg aagacgcggg ccttccccac aacgtgcgca
     1021 ccgtacacgc gcagccacga ctccatcacc gcctcgtgcg gctcaaagcc gctgtctgcg
     1081 cccacggtga tcagcgccgc gcgcttcccg cgcaggatcg aatggtccgt gtagtagccg
     1141 tgggtccggt cgatgaaatc tttcatgcac ccgggagggc cgagccagta gatgggagcc
     1201 ccgaggagat agagatccgc ctggaagaag cgcgccatca gatcctcgaa atcatccccc
     1261 tggatggcgc acctacccag gcgcatgcac gcccgacacc cgcggcaacg ctcgatccgc
     1321 agatcggcca cgcgcacgaa ctccgtgctc gctcctgcgc gctcctccag aagacggagc
     1381 ccctctcgca ccagcaggtc ggtgttcccg tcgcggcgcg gacttcccga aacacccagg
     1441 atcaagctca tctgctgccc ctttcatcca tttcgcatcc atccccttcg cgcttgctgc
     1501 ccgccgtgcc tgcctttggc aagcgcttac tggacacgcg caaggagaag ccccgcctat
     1561 cgcgaagagt tcttcaatag ggagagttgg aacggcaggt cgcgagggct ggcgcccgct
     1621 ttgcgcacag gcctggccac cccgcgatcc gcacactgca attccgaagg gagcctccta
     1681 tggagaagtg gcctatcgga attgcagtg
//
LOCUS       DAOSCV010000980         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47249_length_1704_cov_2.566404, whole genome shotgun sequence.
ACCESSION   DAOSCV010000980 DAOSCV010000000
VERSION     DAOSCV010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47249_length_1704_cov_2.566404"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLY56_18260"
     CDS             <1..309
                     /locus_tag="PLY56_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23473.1"
                     /translation="RKAHMNEVLIGRTSATRLFFMALPVLLAVSQRPPTPVRAVAIQL
                     RPLLLAGVTGSVAAFLLNDSGIIAGGLMLAFVTVGAGYLALSDEPAPEGKSSACASLR
                     "
     gene            291..749
                     /gene="ruvX"
                     /locus_tag="PLY56_18265"
     CDS             291..749
                     /gene="ruvX"
                     /locus_tag="PLY56_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ23474.1"
                     /translation="MRVLAVDYGTKRVGLALSDLLEITVNPLTVLTRKGGDREVIEAI
                     ARIVEAEAVEEVVVGLPVNMDGSHGPAAAAAEAFAAKLAARLKVPVFTHDERLTSEEA
                     EGILMSEGVQRSRRRGRVDPVAAALILRSYLGSPERLARQRSGQRGEEGA"
     gene            804..>1704
                     /gene="mltG"
                     /locus_tag="PLY56_18270"
     CDS             804..>1704
                     /gene="mltG"
                     /locus_tag="PLY56_18270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOJ23475.1"
                     /translation="MSEERQESEAQSPLPEPTPSGLPAETEADPAAPRRQSIWRRLAV
                     AIALLAALAGAGYFGVWTLMPVSTDEKKVVIVEIPRNWGARRIADRLHERGLIRNRTA
                     FLLAGHLLGDLQHLKAGEYELSPSMTPVEILDKLARGDVKAYWVTVPEGVTIKEIAAI
                     FHKMRLIDPTRFVTLATRGGMRIGAEYGVPRKNLEGYLFPDTYKVPRAITERELIRIM
                     VRSFHQQVERGLAQEIAERTNGLSLDETIVLASLVEREARVTEERARIAGVLVNRLRR
                     GMRLECDATVQYALGKTKERLLYS"
BASE COUNT          298 a          509 c          617 g          280 t
ORIGIN      
        1 cgcaaggcac acatgaacga ggtgctcatc gggcggactt cagcgacgcg cctcttcttc
       61 atggcgctcc cggtgctgct ggccgtgagc caacgcccgc cgacgccagt gcgtgccgtg
      121 gcaatacagc tgcgcccgct gcttctggcc ggagtgacgg gaagcgtggc ggcgttcctg
      181 ctgaacgatt ccgggattat cgccggcggc ttgatgttgg cgtttgtgac agtgggggcc
      241 ggctacctgg cgctcagtga cgagccggca ccagaaggga agagctccgc ttgcgcgtcc
      301 ttgcggtaga ttatggcacg aagcgcgttg gcctcgcgct gagcgacctg ctggagatca
      361 cggtcaatcc gctcaccgtt ctgacccgga agggcggtga ccgggaggtt atcgaggcga
      421 ttgcccgaat cgtggaagca gaagcggtgg aggaggtggt cgtcggcctc cccgtcaata
      481 tggatggctc gcacgggcct gcggccgccg cggcggaggc gttcgcggcg aagctggcgg
      541 cgcgcctgaa ggtgcccgtc ttcactcatg atgagcggtt gacttcggaa gaggcagagg
      601 gtatcctcat gagcgagggc gtgcagcgtt cgcgacgccg tggccgggtc gatccagtcg
      661 ccgcggcgct catcttgagg agctaccttg gctcgcccga acgcctcgcc cggcagcgaa
      721 gcggccagcg tggggaggaa ggggcctgag cacaccggaa tcccagcgca ttggaaggta
      781 atcccccgcg ggtaggagat agaatgtccg aagagcgaca agagagcgaa gcccagtctc
      841 cgttaccgga accaacccca tccgggctgc cggcagagac ggaggcggat ccagcagcgc
      901 cacgccgtca atccatctgg cgtcggctcg cggttgccat cgccctgctc gccgcgcttg
      961 ctggcgccgg ctacttcggc gtctggacgc tgatgccggt ctccacggac gagaagaagg
     1021 ttgtgatcgt ggagatcccc cgcaactggg gcgcgcgccg gatcgccgac cgcctgcatg
     1081 aacgggggct gattcggaac cggacggcgt ttcttctggc cgggcacctg ctgggagacc
     1141 ttcagcacct gaaagcaggg gagtacgagc tgagccccag catgacgccc gtggagattc
     1201 tggacaagct ggcgcgtggc gacgtcaagg cgtactgggt gacggtgccg gagggggtga
     1261 ccatcaagga gatcgccgcc atcttccaca agatgcgcct catcgatccg acgcgtttcg
     1321 tgacgctggc gacgcgcggc gggatgcgga tcggcgccga gtatggcgtg cctcggaaaa
     1381 acctggaggg ctacctcttt ccggacacgt acaaggtgcc tcgggctatc accgagcggg
     1441 aactcatccg gatcatggtg cgctccttcc atcagcaggt cgagcgtggt ctggcgcagg
     1501 agatcgcgga gcggaccaac ggcctgagcc tggatgagac gatcgtgctc gcctcgctgg
     1561 tggagcgaga ggcgcgggtt acggaggagc gggcgcgaat cgcgggcgtc ttggtgaacc
     1621 ggctgcgaag agggatgcgc ctggagtgcg acgccacggt gcagtacgcc ctgggaaaga
     1681 cgaaagagcg cctgctctac tcgg
//
LOCUS       DAOSCV010000981         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47418_length_1700_cov_3.343465, whole genome shotgun sequence.
ACCESSION   DAOSCV010000981 DAOSCV010000000
VERSION     DAOSCV010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_1700_cov_3.343465"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLY56_18275"
     CDS             complement(<1..577)
                     /locus_tag="PLY56_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ23476.1"
                     /translation="MAREFPLEKTRNIGIAAHIDAGKTTTTERILFYTGRVHRIGEVD
                     EGAATMDWMAQEQERGITITSAATTCHWRDHRINIIDTPGHVDFTVEVERSLRVLDGV
                     VAVFCAVGGVQPQSETVWRQANRYNVPRIAFVNKMDRTGADFFNVVERMRERLGANAV
                     PVQIPIGAEGDFRGVVDLIEMRAIVYLDELGT"
     gene            complement(801..1271)
                     /gene="rpsG"
                     /locus_tag="PLY56_18280"
     CDS             complement(801..1271)
                     /gene="rpsG"
                     /locus_tag="PLY56_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ23477.1"
                     /translation="MPRKGPAPRREILPDPVYHNVLVARFINRLFIDGKKSVGERIFY
                     GALDQIRDRSGKDPVEVFEQAVRNTMPAVEVKSRRVGGSTYQVPVEVRPARRETLAIR
                     WLITNARRRGGRTMVDKLASELLDAAQGQGASVKKREDTHRMAEANKAFAHYRW"
     gene            complement(1410..>1700)
                     /gene="rpsL"
                     /locus_tag="PLY56_18285"
     CDS             complement(1410..>1700)
                     /gene="rpsL"
                     /locus_tag="PLY56_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ23478.1"
                     /translation="FQRRGVCLSVKTQSPKKPNSALRKIARVRLTNGVEVTAYIPGIG
                     HNLQEHSVVLVRGGRVRDLPGVRYHIVRGTLDAAGTRDRKQGRSKYGTKKPK"
BASE COUNT          285 a          585 c          521 g          309 t
ORIGIN      
        1 cggtgcccag ctcgtcgagg tagacaatcg cgcgcatctc aatcaggtcg acgacgccac
       61 ggaaatcgcc ctcggccccg attggaatct gcaccggaac ggcgttcgcc ccgaggcgct
      121 cgcgcatgcg ctccaccaca ttgaagaagt cggcgccggt gcggtccatc ttgttgacga
      181 acgcgatccg gggcacattg tagcgattgg cctggcgcca gacggtctcc gactgtggct
      241 gaacccctcc gacggcgcag aagaccgcga caacgccatc gagaacgcgc aggctgcgct
      301 cgacctcgac ggtgaagtcg acgtggccgg gcgtgtcgat gatgttgatg cgatggtcac
      361 gccagtgaca ggttgtcgcg gcggaagtga tcgtaatacc gcgctcctgc tcctgggcca
      421 tccagtccat cgttgcggcg ccttcatcca cctcaccgat gcgatgaacg cgccccgtgt
      481 agaagaggat gcgctcggtc gtcgtcgtct tccctgcatc gatatgcgcc gctatgccaa
      541 tattcctggt tttctcgagc ggaaactccc gtgccattac caactcccca ccttcattcc
      601 gggcccgcta ctcggatgct ccaccagtgc acagcagtgc agcaagggcg aacgcgcacg
      661 cagcggtgtg ccagcgtgac agaggcagcg gcaagcgccg gcggcaggca gcaagatcgc
      721 tgccgcgtcc ggcacgccag cgcccgcact cgcgggcact cctcctggcg ccccgacgat
      781 catccggcca catcccgaac ctaccaacga tagtgagcaa acgccttgtt ggcctcggcc
      841 atgcggtgcg tgtcttcgcg cttcttcacc gaggcaccct ggccctgagc agcatccagc
      901 agctcactcg caagcttatc gaccatggtc cggccgcctc gccgccgggc gttcgtgatc
      961 agccaacgga tcgcgagcgt ctcgcggcgc gcaggtcgca cctccaccgg tacctgatag
     1021 gttgatccac caacacggcg cgactttacc tcgaccgccg gcatcgtatt gcggacggcc
     1081 tgctcgaaga cctccacggg atccttccca ctgcggtcgc ggatctgatc aagcgcgcca
     1141 tagaagatgc gctcgccaac gctcttcttg ccatcaataa agaggcggtt gatgaagcgc
     1201 gctaccagca cgttgtggta gaccggatct ggaaggattt cgcgcctggg cgcaggacct
     1261 tttctgggca ttcgttcctc ctcaaacgtg gctcgcagcg cgtattgtgg cgcacggcgc
     1321 gcgatggggc cggcccgcgg tctcccggcc ggggcaggca gcactcgcgc ttgcggggcc
     1381 gttgcgcctc acgcactgcg ctctctctat tacttgggct tcttcgttcc gtacttcgag
     1441 cggccttgct ttcggtcgcg ggtgccggca gcatccagcg tgccccgcac gatgtggtag
     1501 cggacgcccg gaaggtccct gacgcggccc ccgcgaacga gcaccaccga gtgctcctgc
     1561 aggttatgcc cgatgccggg gatataggcc gtcacctcga cgccgttggt caggcgcacg
     1621 cgggcgatct tgcgcagggc cgagttcggc ttcttgggcg actgcgtctt cacgctcagg
     1681 cacacaccgc ggcgctggaa
//
LOCUS       DAOSCV010000982         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47427_length_1700_cov_2.886322, whole genome shotgun sequence.
ACCESSION   DAOSCV010000982 DAOSCV010000000
VERSION     DAOSCV010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47427_length_1700_cov_2.886322"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..722
                     /locus_tag="PLY56_18290"
     CDS             156..722
                     /locus_tag="PLY56_18290"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOJ23479.1"
                     /translation="MPDAPRPIPNAPRPARRVITDPATVEQRARDAARENVSFRAWLK
                     FRCKTPDPEVDALVRETTDQVWRHIDCIACAQCCRCLSITLDDRDIARLARRLHLSIK
                     QFTRRYVEGTGDQRAFNQRPCPFLQSGACSVYSDRPQACRDFPYLHDPGFRHRMLGLL
                     EFSLLCPVIYNTLELLKRRLPWRSRGGR"
     gene            complement(854..>1700)
                     /locus_tag="PLY56_18295"
     CDS             complement(854..>1700)
                     /locus_tag="PLY56_18295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ23480.1"
                     /translation="PGYERARRTAVEARLAEVVKLMGDISHDIKNMITPVLTCAQTLE
                     LLFQQLFMDLDQIYARHEKEHPELIQEVQQAVDFLRSFYPEAIEMFNDGAIATQERVR
                     EIADCVKGIIAQPRFELVDVNDVVARVIKPLSLVAEKAGVALTHERGEVALTLVDQKQ
                     LYNAVYNLVNNAIPETPAGGSVTIRTYDGPEGEFPDGGYVMIEVADTGRGMPEEVRKR
                     LFTEDAVSTKPGGTGLGTKIVKNVVDVHQGRISVESELGKGTRFYMRLPIRTELPEAD
                     QSHAA"
BASE COUNT          287 a          599 c          490 g          324 t
ORIGIN      
        1 cttctcctta tctcggaatc ccaggtgcag atgcgcgccg ggcctgacaa gtcgggcgtt
       61 cacgtagctg gtgggcatcc tgttcacccg atgcgccgat ccccaaggaa ccacgcaacc
      121 gggctgccaa tctagggagt ggaggaactc tatcgatgcc ggatgctccc cgccccatcc
      181 caaacgcccc gcgcccggcg cgccgggtga tcaccgatcc cgccaccgtc gagcagcgag
      241 cgcgtgatgc cgcccgcgaa aacgtctcgt ttcgtgcctg gctcaagttt cgctgcaaga
      301 cacccgatcc cgaggtggat gcccttgtac gcgagaccac cgatcaagtc tggcgccaca
      361 tcgattgcat cgcctgcgcg cagtgctgcc gttgcctgag catcaccctc gatgaccgcg
      421 atatcgcccg cctggcacgc cggctccacc tctccatcaa gcagttcacc cgccgctacg
      481 tcgagggcac cggcgatcag cgtgccttca accaacggcc ctgccccttc ctccaaagcg
      541 gagcttgctc cgtctattcc gaccgcccgc aggcctgccg cgacttcccc tacctgcatg
      601 atcccggctt ccgccatcgc atgctcgggc tcctcgaatt ctcgctcctc tgccccgtca
      661 tctacaacac cctcgaactc ctgaagcggc gccttccctg gcgctcgcgc ggcgggcgct
      721 agccgaagca cccttttagc gcatctaggg agactgatcc atcgtgtttc tgaagggtgt
      781 tacgaccgcc tgtgagcctc ggggatgggc attgccggcc cgagaagcgc tgctccgccg
      841 gtagcgagcg ccgtcaggcg gcgtgagact ggtcggcctc gggaagttcg gtgcggatag
      901 gaagacgcat atagaaacgg gtgcctttgc ccagctcgct ctcgacactg atccgaccct
      961 ggtgaacatc gacgacgttc ttgacgatct tcgtgcccag gccggtgccg cccggctttg
     1021 tgctcacggc gtcctccgtg aagaggcgct tgcgcacttc ctccggcatc ccgcggcccg
     1081 tatccgccac ctcgatcatc acgtagccgc cgtcaggaaa ctcgccctcg gggccgtcgt
     1141 aggtgcgaat cgtcacactg ccgcccgcag gagtctcagg gatcgcgttg ttcaccagat
     1201 tgtagaccgc gttgtagagc tgtttctgat ccaccagtgt cagggcaacc tcgccacgct
     1261 cgtgagtgag agctacacca gccttctccg ccaccagcga gagcggcttg atcacccggg
     1321 cgacgacgtc attgacatcc accagctcga atcgcggctg agcaatgatc cccttcacgc
     1381 agtcagcaat ctcgcggacg cgctcctgag tcgcaatcgc gccgtcgttg aacatctcga
     1441 tcgcctcggg gtagaaggag cggaggaaat cgaccgcctg ctgcacctcc tggatcagct
     1501 cggggtgctc cttttcgtgg cgcgcgtaaa tctgatcgag gtccatgaac agctgctgga
     1561 agagaagctc cagcgtctgc gcgcaggtga gcaccggggt gatcatgttc ttgatgtcgt
     1621 ggctgatgtc gcccatcagc ttgaccacct cggcgagccg cgcctccact gccgtgcggc
     1681 gcgcgcgctc atagccgggc
//
LOCUS       DAOSCV010000983         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47648_length_1694_cov_3.757779, whole genome shotgun sequence.
ACCESSION   DAOSCV010000983 DAOSCV010000000
VERSION     DAOSCV010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47648_length_1694_cov_3.757779"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLY56_18300"
     CDS             complement(<1..201)
                     /locus_tag="PLY56_18300"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOJ23481.1"
                     /translation="MPKSRIMLNMTVQEIREALKETQTVILPIGCVEQHGYHLPISVD
                     IHNAVELATRAAEETGCFVAPPV"
     gene            complement(224..892)
                     /locus_tag="PLY56_18305"
     CDS             complement(224..892)
                     /locus_tag="PLY56_18305"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ23482.1"
                     /translation="MTQVHDISGWIYTGMWSYCPEYPGAEVSELPHPAFLPDDYPVYL
                     QKFVIGGQTGTYIETRAHVDRDAEPVTALPLSEFFRPAVVIHVGEKRRNEPVTVADLE
                     RANPDLRPGDAALLATGWDRFWDDPDYVDGSPYIEREAARWLMDRGIGLLGADFPRFD
                     YVPEPQFPWSEFWERVNLLLAPLIHLEALSGRCGMLVAFPLKIRGACATPCRAAILTH
                     TETP"
     gene            1105..>1694
                     /locus_tag="PLY56_18310"
     CDS             1105..>1694
                     /locus_tag="PLY56_18310"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ23483.1"
                     /translation="MSYIGLDVGTTGCKAVAFDARGRQLASASREYATLSPREGWAEL
                     DSREVGDACLAVIREAAAACSGDPVRGIGISSQGEAFTALGPDGEVLANAMVSSDARA
                     AEIAERWSSEFGREKLYRITGHTAHPMFSLFKLLWVRDHQPDVWQTARHFYCFEELVQ
                     HRLGLEPAISWPLAGRTMLFDVRAHDWSPEILDAVGL"
BASE COUNT          279 a          541 c          588 g          286 t
ORIGIN      
        1 caccggcggg gcgacaaagc acccggtttc ttccgccgcc cgcgtcgcca gctccacggc
       61 gttatggata tcgacgctga tcggcaggtg gtagccgtgc tgctccacgc agccgatggg
      121 gaggatgacc gtctgcgtct ccttcagggc ctcgcggatc tcctggaccg tcatgttcaa
      181 catgatccga gacttcggca tggctctctc caatctctcg catctatggg gtttctgtgt
      241 gcgtcagtat cgcggcgcga cagggagtgg cgcacgcgcc gcggatcttc agggggaagg
      301 cgaccagcat gccgcaccgt ccggagagcg cttccaggtg gatgaggggc gcgagaagca
      361 ggtttacccg ctcccagaac tcgctccagg ggaactgcgg ctccggcacg tagtcgaacc
      421 gcgggaaatc ggcgcccagc aagccgatgc cgcggtccat cagccaccgt gccgcctcgc
      481 gctcgatgta agggctccca tccacgtagt ccgggtcgtc ccagaaccgg tcccatccgg
      541 tggcgaggag cgccgcgtct ccggggcgca ggtctgggtt ggcacgctcc aggtcggcca
      601 cggtcaccgg ctcgttgcgc cgtttctcgc caacgtggat caccacggcg gggcggaaga
      661 actctgagag gggcagggcg gtgaccggct cggcgtcgcg gtccacatgc gcgcgcgttt
      721 cgatgtaggt gcctgtctgc ccaccgataa cgaacttctg gaggtagacg gggtagtcgt
      781 ccggcaagaa agcgggatgc ggcagctcgg agacctccgc ccctggatac tccgggcagt
      841 aggaccacat cccggtgtag atccacccac tgatatcatg cacctgggtc atcagaattc
      901 actctccgcc agcgatctca cgcgacaggg gggtgcgccc gggcgtccga agcgcctggg
      961 atcagttccc gctggcctag gcggcctcct gcccgaggcg acatcgcgcg gcaggaaagc
     1021 cgggcggaac aggggaacta agggggtgca gaaacgggtg gctatccacc tgcaatctga
     1081 gacccaggat cgggagagcg tgccatgagc tacatcggtt tggacgtggg caccaccggg
     1141 tgcaaggcgg tggctttcga cgcgcgaggc cgccaactcg cttcggcatc gcgggaatat
     1201 gcgacactca gcccccggga agggtgggcc gagctagact cgcgcgaagt gggcgatgcc
     1261 tgcctagccg tcattcgcga ggcagcggcg gcgtgctcgg gcgaccctgt gcgcgggatc
     1321 gggatctcca gccagggcga ggcgtttacg gctttggggc cagacggcga ggtgctcgcc
     1381 aacgcgatgg tctcgtcgga tgcccgcgcg gcagagatcg ccgagcgttg gagcagcgag
     1441 tttggtcgcg agaagctcta tcgcatcacc gggcacaccg cccatccgat gttctccctc
     1501 ttcaagctgc tgtgggtgcg ggatcaccag ccggacgtgt ggcagaccgc ccggcatttc
     1561 tactgcttcg aggagctggt gcagcaccgg ctcggcctcg agcctgctat tagctggccg
     1621 ctggcggggc gcaccatgct gttcgacgtg cgcgcgcacg actggtcgcc cgagatcctg
     1681 gacgccgtcg ggct
//
LOCUS       DAOSCV010000984         1692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47739_length_1692_cov_3.843005, whole genome shotgun sequence.
ACCESSION   DAOSCV010000984 DAOSCV010000000
VERSION     DAOSCV010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47739_length_1692_cov_3.843005"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLY56_18315"
     CDS             <1..604
                     /locus_tag="PLY56_18315"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOJ23484.1"
                     /translation="QFVGIKAARLLPLLALGAILVGGLFTPANDWKSRRAGVAANLRA
                     LLAEPVRVWQIVAIGAAVLLLLILLLRTGNEPGVGVSGLELKLRSLMDRVLFVRPRTK
                     EFLIGHPAMVIALWAALAGWRPVWAPALLIGGIGQVSVLNTFCHIHTPFLVSLLRTTN
                     GLVLGIVLGLAVITIAQRLWVSNSKGLPVKAPAQKGSGPE"
     gene            601..>1692
                     /gene="csaB"
                     /locus_tag="PLY56_18320"
     CDS             601..>1692
                     /gene="csaB"
                     /locus_tag="PLY56_18320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HOJ23485.1"
                     /translation="MSARVVISGYYGFDNAGDEAVLAGLIDAFQAAVPELSIAVASAN
                     PEATRRQHGVDAFHRYRWREFRAQLQACDVFVSGGGSLFQDVTSRRSIYYYLGTLALA
                     RLCHRPVMICGQGIGPIISPIARRATAALFNRAAAITVRDPASLETLAALGVRRPRIE
                     QTADPVFALRPSPREVGKEILARHGIDLNRPCIAFALREWRPRAGSAPAATDPNDEHI
                     LGIWAEAAAFAHRELGAQVLFVPMHPPDDESYARRVAEQAAVPAVVLPGPCPPRDLIS
                     VVGCVDLMVAMRLHALIFAALQGVPLVGISYDPKIDGFLRLLDRTPAAEMTSIEGEVL
                     LHAIQTAWDARAGEASSLKQKAEHLRENAL"
BASE COUNT          246 a          552 c          577 g          317 t
ORIGIN      
        1 ccagttcgtc gggatcaagg cggcacgtct cctcccgctc ctggcattgg gggcgatctt
       61 ggtgggcggt ctcttcactc ccgcaaacga ctggaagagc cggcgcgccg gagtcgcggc
      121 taacctgcgc gcgctcctgg cagaaccggt ccgcgtgtgg cagatcgtcg ccatcggcgc
      181 ggcggtgctg ctcctgctca tcctcttgct gcgcaccggc aacgagccgg gcgtcggcgt
      241 ctcgggattg gagctgaagc tacgctcgct gatggatcgg gtgctgttcg tgcgcccgcg
      301 gaccaaggag tttctgatcg gccacccggc gatggtgatc gccttgtggg ccgccctcgc
      361 gggatggcgg cctgtctggg cgccggcgct cctgatcggt gggattggcc aggtttcggt
      421 gctgaacacc ttctgccata tccacacgcc gttccttgtc tcgctcctgc gcacaacaaa
      481 tggactggtg ctgggaatcg tgctgggcct cgccgtgatc actatcgcgc aacggctatg
      541 ggtctcgaac tccaaggggc tccccgtgaa ggcgcccgcg cagaagggga gcgggcctga
      601 gtgagcgcgc gtgtggtcat ctctggctac tacgggttcg ataacgccgg cgatgaggcg
      661 gtgctcgccg ggctgatcga tgccttccag gcagcagtgc ccgagctctc gattgccgtg
      721 gcttcggcca acccggaggc cacgcggcgc cagcacggcg tggatgcctt ccatcgctat
      781 cggtggcgcg agtttcgagc gcagcttcag gcatgcgacg tcttcgtgag tggtggcggc
      841 tcgctcttcc aggatgttac cagccgacgc tccatttact actacctggg aacgctggcc
      901 ctggcgcgcc tctgccatcg gccagtgatg atttgcggcc aggggatcgg gccgatcatc
      961 agtcccatcg ctcggcgggc gacagcggca ctcttcaacc gtgccgcggc gatcaccgtt
     1021 cgcgatcccg catccctgga gacgctggcg gcgctcggag tgcgccggcc ccggatcgag
     1081 cagaccgccg atccggtttt cgcgctccgg ccttcgcccc gcgaggtggg caaggagatc
     1141 ctggcccggc atggtatcga tctcaaccgg ccttgcatcg cgttcgccct ccgcgagtgg
     1201 cggccgcgcg ccggtagcgc gccagcggca acagatccga acgacgagca catcctgggt
     1261 atttgggcgg aggccgccgc gttcgcacat cgcgagttgg gggcgcaggt gctcttcgtg
     1321 ccgatgcacc cccctgacga cgagagctac gcgcggcgcg tggctgaaca ggccgcggtg
     1381 ccagccgtcg tgctccccgg gccatgccct ccccgcgatc tgatatcggt ggtggggtgc
     1441 gtggatctga tggtcgcgat gcgcctgcac gcgctgatct tcgcggcgct gcagggggtt
     1501 ccgcttgtcg ggatctcgta tgatccgaaa atcgatggct tcctccgctt gctggataga
     1561 acaccggccg cggagatgac gtctatagaa ggggaggtac tgctgcacgc cattcaaacg
     1621 gcctgggatg cgcgcgcagg agaagcctcc tcgctgaagc aaaaggccga acatctccgc
     1681 gagaacgccc tg
//
LOCUS       DAOSCV010000985         1691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47790_length_1691_cov_3.474328, whole genome shotgun sequence.
ACCESSION   DAOSCV010000985 DAOSCV010000000
VERSION     DAOSCV010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47790_length_1691_cov_3.474328"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1691)
                     /gene="mfd"
                     /locus_tag="PLY56_18325"
     CDS             complement(<1..>1691)
                     /gene="mfd"
                     /locus_tag="PLY56_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ23486.1"
                     /translation="LQGALSAGFLLPCLKLAVLTDSEVLGARKIRRPRRVARQGQRIT
                     SLSQLTEGDYVVHLHHGIGRYRGLVTQNVDGAAKDYLHLEYADNTRLYVPVDQIDRVT
                     RYIGSDDHPPQIHRIGGTEWMQTKRRARAKVREMAKELVELYAARQAAQGYAFSPDTP
                     WQREMEESFIYEETPDQLTAIREMKEGMERPQPMDRLICGDVGYGKTEVAIRGAFKAV
                     MDGKQVAVLVPTTVLAQQHFNTFTERLAAFPVRVEMLSRFRSPRDQARVVKGLREGTV
                     DIVIGTHRLLSKDVAFRDLGLVIVDEEQRFGVAHKERLKQLRTSVDVLTLTATPIPRT
                     LHMSLSGIRDMSTISDPPEGRTAIRTYCVERNDELIRDAILRELDRDGQVYFVHNRVE
                     TIEAVAQQLQNLVPQARFAIGHGQMPEGRLEKVMLDFYDRRYDVLVCTTIIESGLDIP
                     NVNTILIDNAPHMGLAQLYQLRGRVGRSPRQAYCYLLFDPKRLLTETAEKRLDAIREF
                     TQLGSGFQVALRDLEIRGAGNLLGAEQHGFMLSVGFELYCRMIEEAVKSLRGEEVT"
BASE COUNT          296 a          536 c          550 g          309 t
ORIGIN      
        1 gtgacctcct cgccgcgcaa cgatttcact gcctcctcaa tcatccggca gtagagctcg
       61 aaaccaacgg agagcatgaa gccgtgctgc tcggccccga ggaggttccc ggcgccgcga
      121 atctccaggt cacgcagtgc aacctggaag ccggatccca actgggtgaa ctcgcggatc
      181 gcatccaggc gcttctccgc cgtttcggtg aggagacgct ttggatcaaa gagcaggtag
      241 cagtaggcct ggcgtggcga ccggccgacg cgcccgcgga gctgatagag ctgcgcgagc
      301 cccatatggg gggcgttatc gatcaagatg gtgttgacgt tggggatgtc gagcccgctt
      361 tcaatgatcg tggtgcagac gagcacgtcg tagcggcggt cgtagaaatc gagcatcacc
      421 ttctcaaggc gcccctccgg catctgccca tgaccaatgg caaagcgcgc ctggggaacc
      481 aggttctgca gctgctgagc caccgcctca atcgtctcga cgcggttgtg gacgaagtag
      541 acctggccat cgcggtccag ctctcggagg atcgcatcgc ggatcagctc atcgttgcgc
      601 tccacacaat aggtccggat cgctgttcgc ccctcgggcg ggtcgctgat agtcgacata
      661 tcgcgaatgc ccgagagtga catgtgcagc gtgcgcggga tcggcgtggc ggtgagggta
      721 agcacatcca cgctggtgcg aagctgcttc agccgctcct tgtgcgctac tccgaagcgc
      781 tgctcctcat cgacgatcac gaggccaagg tcgcgaaacg ccacatcttt cgagaggagg
      841 cggtgcgtgc cgatgacaat atcgaccgtg ccctcgcgga ggcccttcac cacgcgcgcc
      901 tgatcgcgtg gggatcggaa gcgcgagagc atctccacgc gcacggggaa ggccgccagg
      961 cgctcggtga aggtgttgaa gtgctgctgt gcgagcacgg ttgttggcac gaggacggcg
     1021 acctgcttgc catccatcac cgccttgaaa gccccgcgaa tggccacctc agtcttgcca
     1081 tagccgacat cgccgcagat gagccggtcc atcggctggg gccgctccat gccctccttc
     1141 atctcgcgaa tggcggtgag ctgatcgggc gtctcctcgt agatgaagct ctcctccatc
     1201 tcgcgttgcc agggagtgtc tgggctgaag gcgtatccct gcgccgcctg ccgggcggca
     1261 tacagttcca ccagctcctt ggccatctcg cgcactttgg cgcgcgcgcg ccgcttcgtt
     1321 tgcatccatt cggtgccccc gatgcggtgg atctgcggcg ggtgatcatc cgagccgatg
     1381 tagcgggtga cgcggtcaat ctggtccacc ggcacataga ggcgagtgtt gtcagcatac
     1441 tccaggtgga ggtaatcctt cgcggcgcca tccacgttct gcgtgaccag gccccgatag
     1501 cggccgatcc cgtggtgcag gtgcaccacg tagtcgcctt ctgtcagctg cgagagcgag
     1561 gtgatgcgct ggccctggcg cgcaacgcgc cgcggccggc gaatcttgcg tgccccaagc
     1621 acctcgctgt cggtgaggac ggccagcttc aggcagggga ggaggaaacc cgcggagagc
     1681 gccccctgaa g
//
LOCUS       DAOSCV010000986         1689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47878_length_1689_cov_2.833537, whole genome shotgun sequence.
ACCESSION   DAOSCV010000986 DAOSCV010000000
VERSION     DAOSCV010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47878_length_1689_cov_2.833537"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLY56_18330"
     CDS             <1..462
                     /locus_tag="PLY56_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOJ23487.1"
                     /translation="IAVEASVLRDSRLAKEVRVGPFAHLRPGSEVGARTKIGDYVELK
                     NAQVGEGVSIAHLAYVGDATVGDRVNIGAGVITCNYDGIRKNRTTIGDDAFIGSNSVL
                     IAPVTIGSGAYVAAHSSITEEVPADALGIARSRQENKPDWARRRRESKQKG"
     gene            466..1434
                     /locus_tag="PLY56_18335"
     CDS             466..1434
                     /locus_tag="PLY56_18335"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ23488.1"
                     /translation="MDRANELKVFSGNANKQLARAICTHLGIEPVRGQVTRFSDHEIR
                     VEIDESVRGDDVFIVQPTCAPVNDHLMELLISIDAFRRASAARITAVIPYFGYARQDK
                     KIKAREPISAKLVATLITAAGAHRVLTMDLHALQIQGFFDFPVDHLHAGPIMADHLIE
                     CGLCGHDVVVVSPDVGGVSMARAFADRIGADFAIVVKRRPGPNRVEVIEVIGDVKDRT
                     CVLVDDMVDTAGSLLGGVNALFERGARAACACATHPVLSGNAIERIQASPLRQLVVTD
                     TIPISEDRLSPKIEVLSVAPVLAEAIRRIHENASVSTLFRNHGKSD"
     gene            1528..>1689
                     /locus_tag="PLY56_18340"
     CDS             1528..>1689
                     /locus_tag="PLY56_18340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ23489.1"
                     /translation="MKIILGLGNPGAEYASTRHNVGFMTADLLAKRHGIRLTTRRHSS
                     LLGEGTIAGV"
BASE COUNT          316 a          543 c          532 g          298 t
ORIGIN      
        1 atcgccgtgg aggcctctgt tttgcgcgac tcgcgcctcg ccaaagaggt acgcgtgggc
       61 cccttcgcgc acctgcgtcc agggtcggaa gtgggcgccc gcaccaagat cggcgactat
      121 gtggaactca agaacgctca ggtaggagag ggcgtttcca tcgcgcacct cgcctacgtg
      181 ggtgatgcca ccgtgggcga ccgggtgaat atcggggcag gcgtcatcac ctgcaactac
      241 gatgggatcc ggaagaaccg gacgacgatt ggcgatgacg ccttcatcgg gagcaactcc
      301 gtcctcatcg caccggtcac catcgggtct ggggcctacg tggccgcgca ttcctctatc
      361 accgaggagg tccctgcaga cgcgcttgga atcgctcgct cgcggcagga gaacaagccg
      421 gattgggcac gccgccgccg tgagtccaag cagaaggggt gagcgatgga ccgggcgaac
      481 gagttgaaag tcttcagtgg gaacgccaac aagcagctag cccgggcgat ctgcacgcac
      541 ctaggcatcg agccggttcg cggtcaggtc acccgcttct ccgatcacga gatccgcgtc
      601 gagatcgatg agagcgttcg cggcgacgac gtcttcatcg tgcagccaac gtgcgcgccc
      661 gtgaatgacc atttgatgga gctgctgatc tccatcgatg ccttccggag agcatccgcg
      721 gcgcgtatca cggctgtgat cccctacttt ggctatgccc gccaggataa gaagatcaag
      781 gcgcgcgagc cgatcagcgc caagctagtc gccacgctga ttaccgccgc cggggcacac
      841 cgcgttctga cgatggacct ccacgccctg cagatccagg gcttcttcga ctttcccgtg
      901 gatcaccttc atgcggggcc gatcatggcc gaccacctga tcgaatgcgg gctctgcgga
      961 cacgacgttg tcgtcgtctc ccccgacgtc ggcggcgtct cgatggcacg cgccttcgcc
     1021 gaccgcatcg gtgccgattt cgccatcgtg gtcaagcgcc ggcccgggcc aaatagggta
     1081 gaggtcatcg aggtgatcgg cgacgtcaag gatagaacgt gcgtgttggt ggatgatatg
     1141 gtggatacgg ccggctcgct actcggtggc gtgaacgcgc tcttcgagcg cggggcgcgg
     1201 gccgcgtgcg cgtgtgccac ccatcccgtt ctctccggga acgccatcga acgcatccag
     1261 gcctctccgc ttcgccagct ggtcgtcacc gataccattc ccatcagcga ggaccggctc
     1321 tcacccaaga tcgaggtcct ctccgtcgcc ccggtcctgg cggaggccat ccggcgcatc
     1381 catgagaacg cctcggtcag cacgctcttc aggaaccacg ggaaaagcga ttgaacacct
     1441 tccctgcacc gccgggtggc gcttcccggt gcgagccagg ggtctcagaa cgagttttcc
     1501 aaagcgaata gtacgagcaa gacgatgatg aagattatcc tggggctggg aaaccccggg
     1561 gcagagtatg cgtccacacg gcacaacgtg ggcttcatga cagcggacct gctggcgaag
     1621 cgccacggga tccgcctcac gacgcgacgc cactcttctc tgttaggaga aggcactatc
     1681 gccggcgtc
//
LOCUS       DAOSCV010000987         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_47963_length_1687_cov_3.280025, whole genome shotgun sequence.
ACCESSION   DAOSCV010000987 DAOSCV010000000
VERSION     DAOSCV010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47963_length_1687_cov_3.280025"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1687)
                     /locus_tag="PLY56_18345"
     CDS             complement(<1..>1687)
                     /locus_tag="PLY56_18345"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HOJ23490.1"
                     /translation="PEEARRYLGYGCDVGILPYRLQDDYDRQRQPRPLRVAVLENEWL
                     RATFLLDLGGRLWSLIHKPTGRELLYRNPVFQPGNLAVRNAWFAGGVEWNFGIRGHTP
                     FTCAPLFAARATSPHGAPILRLYEWERIRQAPYQLDFWLPDGSPFLFARVRLLNPHNR
                     EIPSYWWSNIAVTQREDVRVIVPADGAYNFSYAGKFARVAIPDGPGFDATYPTNAPRA
                     CDYFFDIPDGRRPWIAALDAEGRGLIQSSTERLRGRKLFVWGMGPGGQRWQEFLAEPG
                     YAYCEIQAGVARTQYECFPMPPQSEISWVEVYGLMEADPAKVHGNDWQQARDAVDERL
                     EAMLPDERVRAEWARVQPALEAPPDEILSRGSGWGALERLRRARAGETASFPKAIPFD
                     DASLGEDQVPWLALLEEGALPAGDPGKTPGAWLVQDEWRALLDEAVTAGRGDHWLSWL
                     HLGVMRYSTGEVEAARQAWERSLALAPSAWALRCLAVLAAHEKRAGDAADLWFQAHAL
                     APECRPLAVECCQALIEAGRPAEALSLVDRLSPELRGHGRVRLLEARAALDAGE"
BASE COUNT          289 a          599 c          565 g          234 t
ORIGIN      
        1 ctcaccggca tccagggcag cgcgagcttc cagaaggcgg acgcgcccat gcccgcggag
       61 ttcgggagag agccggtcca ccagcgacag cgcctccgcc ggtcggccgg cttcgatgag
      121 cgcctggcag cactccacgg caagcggccg gcactccggc gccagcgcgt gcgcctggaa
      181 ccagaggtcg gcagcgtccc ctgctcgctt ctcgtgggct gctagaacgg ccaaacaacg
      241 cagcgcccat gccgagggcg ccagcgcaag cgagcgctcc catgcctgcc gggcggcctc
      301 cacttcgccg gtgctataac gcatcacccc caggtggagc catgagagcc aatggtcccc
      361 gcggccggca gtcacggctt catccaggag cgcgcgccac tcatcctgga cgagccatgc
      421 ccctggggtc ttgcccggat ccccggctgg cagcgcgccc tcctccaaga gcgcgagcca
      481 gggtacctga tcctcgccca gagaggcatc gtcgaagggg atcgccttcg gaaaagacgc
      541 cgtctcgccg gcacgcgcgc gccggagccg ttcgagcgcc ccccagccgg agccgcggct
      601 cagaatctcg tcgggaggcg cttccagcgc cggctggaca cgcgcccact ccgcgcgcac
      661 gcgctcgtcg gggagcattg cctccaggcg ctcatccacc gcgtcgcgcg cctgttgcca
      721 atcgtttccg tggaccttcg ctggatcggc ttccatcaac ccatagacct ccacccagga
      781 gatctcggat tgaggcggca tggggaagca ctcgtactgg gtgcgcgcca cccccgcctg
      841 gatctcgcag taggcgtagc ccggttccgc gaggaactcc tgccagcgct ggccgccagg
      901 gcccatcccc cacacgaaga gcttgcgccc gcgcaggcgc tcggtggagc tctggatcag
      961 cccgcggcct tcggcatcca gggccgcgat ccacggccgg cgcccgtcag gaatatcgaa
     1021 gaagtagtcg caggcgcggg gcgcgttcgt gggataggtg gcatcgaacc cggggccatc
     1081 ggggatcgcc acgcgggcga acttgccggc atagctaaag ttgtaggcgc catccgccgg
     1141 cacgatcacg cgcacgtcct cgcgctgcgt tacggcgatg ttcgaccacc aatagctcgg
     1201 gatctcgcgg ttgtgcgggt tcaagaggcg cacgcgcgcg aagaggaacg gcgagccatc
     1261 cggaagccag aagtcgagct gatagggcgc ctggcggata cgctcccact catagaggcg
     1321 caggatgggc gcgccatggg gtgacgtcgc gcgcgccgcg aagaggggcg cgcaggtgaa
     1381 cggcgtatgc ccgcgaatgc cgaagttcca ctccacgcct ccggcaaacc aggcgttgcg
     1441 cacggccagg ttgcccggct ggaagacggg attgcgatag agcagctcgc gcccggtagg
     1501 cttatggatc agcgaccaga gccgcccgcc caggtcgagc aggaaggtcg cacgcagcca
     1561 ttcattctcc agcacagcca cccgaagggg cctcggctgg cgctggcggt catagtcgtc
     1621 ctggagacga tagggcagga ttccaacgtc acatccatag ccgaggtagc gccgcgcctc
     1681 ctccggc
//
LOCUS       DAOSCV010000988         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48154_length_1683_cov_2.695946, whole genome shotgun sequence.
ACCESSION   DAOSCV010000988 DAOSCV010000000
VERSION     DAOSCV010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48154_length_1683_cov_2.695946"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1673
                     /locus_tag="PLY56_18350"
     CDS             87..1673
                     /locus_tag="PLY56_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23491.1"
                     /translation="MRQGELIWYCCAVAGLGLMLAGCGSVDGDHTKLTPDPSGAPTVA
                     VQPGSGLAGVTLSASYKAATDVLGQPTSTEPRQTDGPPAVLATWSDKGVAALFFDAND
                     DGQVADTEQNVMLLATAPFAGSTVDGIAIGSPQAAVHAAYGTPDETTRGSTEWYLKRG
                     LVIGYDAEKKVESFGIFTPQLENRTESEIIPGRSVGSVFLGGAYGAAESRYGTPAKVV
                     PYQDQDQAVVFAYWPTRGILACYDDADKNGVLASSERCVLAYAALPFGGRTAEGVGTD
                     ATKAIVRSTYPPDAEAEGDYEEELLDNGGSADWYWRRGLVFNYNAQGGVTSIGVFTPL
                     QAGEIGLGREIVPGKGVGPALIGRTYAELCAVLDLPEESSFKVQPVGDGQGVLAIWPL
                     YRISVMFKDANANGRPDADEVAHMVWARTPYAGHTPEGIGTDADRARVRSAFGTPEET
                     AGAMDWYWSRGIMFTYAEDGKVLSIGVFEPVTRAVAGLRRAASVSAEQPASQSSQAIN
                     RAIGTKMPLGFNLKGLFRAP"
BASE COUNT          283 a          525 c          596 g          279 t
ORIGIN      
        1 gcggcgagga gggcaaccac cgaacggcgc ccgtgacggc catccccgtg tgcaccgccc
       61 ggataaggcc gaagggagag aaacggatga gacaagggga actcatttgg tattgctgtg
      121 cggtcgcagg actcggtctc atgcttgccg ggtgtggcag cgtggacggc gatcacacca
      181 agctcacgcc cgacccgtcg ggtgcgccca cggttgcggt tcaaccggga agtggattgg
      241 cgggcgtgac gctgagcgcc tcgtacaagg cggcgactga cgtgcttggc cagccgacgt
      301 ctaccgagcc taggcagacc gatgggccgc ccgcggtgct cgcgacctgg tctgacaagg
      361 gcgttgccgc gctcttcttc gatgcgaacg acgatggcca ggtagccgat acggagcaga
      421 acgtgatgct cctcgccacg gctccgtttg ccggctcgac cgtggatggg attgccattg
      481 gcagccccca ggccgccgtg cacgcggcgt atggcacccc ggacgagacg acgcgcggtt
      541 ccaccgagtg gtacctgaag cgcgggcttg tcatcgggta tgacgccgag aagaaggtcg
      601 agagcttcgg tatcttcacc ccgcagctgg agaaccgtac cgagtccgag atcatccccg
      661 ggcgctcggt ggggtccgtc ttcctgggcg gagcatacgg cgccgccgaa tctcgctatg
      721 gcacgccggc caaggtggtg ccctaccagg atcaggatca ggctgtggtc ttcgcatatt
      781 ggccgacccg gggtatcctc gcgtgttacg atgacgccga taagaatggc gtcctcgcca
      841 gctccgagcg gtgcgttctg gcttatgccg cgcttccgtt cggtgggcgc accgccgagg
      901 gggtaggaac ggacgcgacg aaagcgattg ttcgctcgac ctacccgccg gatgcggagg
      961 ccgaaggtga ctatgaggaa gagctgctcg acaatggcgg gtccgcagat tggtactggc
     1021 ggcgcggtct cgtcttcaac tacaacgcgc agggtggggt gaccagcatt ggtgtcttca
     1081 cccccctcca ggccggcgag atcggtcttg gccgcgagat cgtgcccgga aagggcgtgg
     1141 gcccggcgct gattggccgc acctacgccg agctctgcgc ggtgctcgat ctgccggagg
     1201 aatccagctt caaggtccag cccgtgggcg atggccaggg tgtgcttgcg atctggccgc
     1261 tgtaccgcat ctcggtgatg ttcaaggatg cgaacgccaa cggcaggccc gacgctgacg
     1321 aggtcgccca catggtctgg gcacgcacgc cgtacgccgg ccacacgccc gaggggatcg
     1381 gtacggatgc ggaccgcgcg cgcgtgcgct ccgccttcgg aacccccgag gagaccgcgg
     1441 gtgcgatgga ctggtactgg tcgcgcggta tcatgttcac ctacgccgag gatggcaagg
     1501 tgctgagcat cggcgtgttc gagccggtca cgcgggccgt cgccggcctt cgccgtgcgg
     1561 cgagcgttag cgcggagcag ccagcgagcc agtccagcca ggcgatcaac cgggccatcg
     1621 gcacgaagat gccgctgggc ttcaatctga aggggctctt ccgcgcgccc taagacgcgg
     1681 agt
//
LOCUS       DAOSCV010000989         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48215_length_1681_cov_3.218327, whole genome shotgun sequence.
ACCESSION   DAOSCV010000989 DAOSCV010000000
VERSION     DAOSCV010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48215_length_1681_cov_3.218327"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLY56_18355"
     CDS             complement(<1..207)
                     /locus_tag="PLY56_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23492.1"
                     /translation="MKWDRKRAFCVSLVFAVVLVPAAFIPTVREAPWFKLLVAFAFMT
                     VVVALISFLKARGASEDETSERPRR"
     gene            complement(274..852)
                     /locus_tag="PLY56_18360"
     CDS             complement(274..852)
                     /locus_tag="PLY56_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23493.1"
                     /translation="MEVRPRTVEEVDERQRTLREEFRRLNNALESKFGEAITKRQILD
                     AAQACEVLRQLARGLKVLSDESDGMREPEAWQKALERFVSGRMDDKVGAIQSEEIGKL
                     KDDLNAIQAELANLREQLETGVNHALQKIADAQGKVDVTDGLLKEAIRQRDDLSQKIE
                     RLEEIARELDACERMAEVAGAEPAGGQWGDHG"
     gene            complement(865..>1681)
                     /locus_tag="PLY56_18365"
     CDS             complement(865..>1681)
                     /locus_tag="PLY56_18365"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ23494.1"
                     /translation="EEQAAGDQMDPVEYERWLQQQLRRVLREARHQLSGETSFEFSVP
                     WAEESLGRLTRDHLERWASFDTEITAAIQMAVQGARDRDAPNGLRNDQIDRVLITGGL
                     NRSPLIRRLVDHFPHVEPCEDGFEEYAVAYGAARCAAILTGRCEGVVFRPRETPAFGI
                     RTGIDTFDKVIPENAPPGFKAKKVYTLPNRYASQMVLEVCQGFSRRFHEELSLGAKEL
                     ELKFPEGTSKGSYLEREIHVEMELADTRLLVTVIDRTRGISQPVPDKLELEAR"
BASE COUNT          276 a          616 c          454 g          335 t
ORIGIN      
        1 gcggcgcggt cgctcactcg tctcatcctc ggacgccccc ctggccttca aaaagctgat
       61 caaggcaacg accactgtca taaacgcaaa ggctaccagg agcttaaacc acggggcttc
      121 ccggaccgtc gggatgaacg cggcagggac aagcacgacc gcgaacacca gcgacacaca
      181 gaatgccctt ttcctgtccc acttcatctt cgagcccgcc gcctctctcc ctggcgttcc
      241 gggatagcaa accgcgccac acatccgggg cggttacccg tgatcccccc actggccgcc
      301 ggctggctca gccccagcga cctccgccat cctttcgcaa gcatccagct ccctggcgat
      361 ctcctcgagg cgctcgattt tctgggaaag atcgtcccgc tgcctgatgg cctccttcag
      421 gagaccatcg gtcacatcta ccttcccttg cgcatccgct atcttctgaa gcgcatggtt
      481 gaccccagtc tctagctgct ccctcagatt ggccagttcc gcttgtatcg cgttgagatc
      541 atccttgagc ttgccaatct cctcgctctg gatggcaccc accttgtcat ccatgcggcc
      601 gctgacgaac ctctcgagcg ccttctgcca tgcctcgggt tcccgcatcc cgtcactctc
      661 atcgctcagc accttcagtc cccgagctaa ctgtcggagc acctcgcacg cctgcgcggc
      721 atccaggatc tgacgcttcg tgatcgcttc tccaaacttg ctttcaagcg cattgttgag
      781 ccggcgaaac tcctcccgca gcgtcctctg cctctcgtcg acctcttcca ccgttctcgg
      841 acggacttcc atgagattcc tccttcacct cgcctcgagc tccagcttgt cgggcaccgg
      901 ctgggaaatc cccctggtgc ggtcgatcac cgtgaccagg agtctggtat cggcgagctc
      961 catctcaacg tggatctcgc gctctaggta gctgcccttg ctcgtgccct cgggaaactt
     1021 gagctcaagc tccttcgcgc ccagcgacag ctcctcatgg aaccgccggg agaagccctg
     1081 gcacacctcc agcaccatct ggctggcata gcggttcggc agagtgtaga ccttcttcgc
     1141 cttgaaccct ggcggcgcgt tttccgggat caccttgtcg aaggtgtcga tcccggtgcg
     1201 gatcccaaac gcgggcgtct cgcgcgggcg gaagaccaca ccctcgcacc ggcccgtgag
     1261 gatcgccgcg caccgcgccg cgccgtaggc gaccgcgtac tcctcaaacc catcctcgca
     1321 cggctccacg tgggggaagt gatccacgag ccggcggatc aaaggcgagc ggttgagccc
     1381 accggtgatc aggacgcggt ctatctggtc attccggagg ccgttcgggg catcgcggtc
     1441 gcgcgcgccc tgcaccgcca tctggatcgc cgcggtgatc tcggtatcga agctggccca
     1501 tcgctccaga tggtcgcgtg tcagccggcc gaggctctcc tcggcccagg gcaccgaaaa
     1561 ctcgaaggag gtctcgccgc tcaactggtg tcgcgcctcg cgcaggacgc gccggagctg
     1621 ttgctggagc cagcgctcat actccactgg gtccatctga tccccggcgg cctgctcttc
     1681 t
//
LOCUS       DAOSCV010000990         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48350_length_1678_cov_3.902649, whole genome shotgun sequence.
ACCESSION   DAOSCV010000990 DAOSCV010000000
VERSION     DAOSCV010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48350_length_1678_cov_3.902649"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLY56_18370"
     CDS             <1..251
                     /locus_tag="PLY56_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23495.1"
                     /translation="REGYDDYRYIYTLRQLIAQAKRSPRPAAKKAAAEAEKELQFVWD
                     SIRVQAKYKHDDLWTPTEFDVNRWLIAQQILAVRQALK"
     gene            complement(441..>1678)
                     /locus_tag="PLY56_18375"
     CDS             complement(441..>1678)
                     /locus_tag="PLY56_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23496.1"
                     /translation="WGYARAGGEGTPEARAARALGHLGPITVVLGPWQRLEPDARVVA
                     AGKYPRLPEGAPRAPGACPDFDVEAFLSRAHREPYWEQLGKEVAQIRRACPNVRIMQM
                     IHPAMEVIYKPLQHRWPIASDAIQGAGGATFEDAGYSRSWVRAMTEKEWGVLYYVPRP
                     GSAYLEEILEGMRRALDQYGADGIYCDEFSWAFTRRGYSRYDYSRWDGYSADLDAEGK
                     VIRLKCDNAYVTEATQLRMAHEVLGRGKFFLGNGAAALRSVNSLPIARFIEGGNGYGA
                     MAFGHLSPTPLVLGNMGDQTTRKGVFESVRACLSQGCIYSPTAVNLLLEGPDNFVCKL
                     YPITVREIGPGWVMGEERLTTTVSGTYRWPGRATKLRLYRYNAEGELQRPVPVVEAGP
                     EFRAEVPERGLLIAEVAGP"
BASE COUNT          284 a          585 c          547 g          262 t
ORIGIN      
        1 accgcgaggg gtatgacgac taccgctaca tctacacgct gcgccagctg atcgcgcagg
       61 cgaagcggag cccccgcccg gcggcaaaga aggcggccgc cgaggccgag aaggagctgc
      121 agttcgtgtg ggactccatc cgcgtccagg ccaaatacaa gcacgatgac ctgtggacgc
      181 ccacggagtt cgatgtcaac cgctggctaa tcgcgcagca gatcctggcg gtgcgccagg
      241 cgctgaagta gccgggcagg aatggatggc gggcgttgcg cgggtgttcg catccgcgca
      301 acgcatccct ctcccattcc gcgccagcgg ggatcgccca ggaatgcagc ctggcccagc
      361 ccggcagggc ttcgctcaca agcagcgcgg tgattggtct tccatgcgat gccgcgctgg
      421 cgccgaaccg ccggcgcggg ctatggtccg gccacctcgg caattagcag cccccgctcg
      481 ggtacctcgg cgcgaaactc cgggccggct tctaccacgg gaaccgggcg ctgtagctcg
      541 ccctcggcgt tataccggta gaggcgcagc ttcgtggcgc gcccgggcca gcggtaggtg
      601 ccggagacgg ttgtcgtcag gcgctcctcg cccatcaccc accccgggcc aatctcgcgg
      661 accgtgatcg ggtagagctt gcagacgaag ttgtccggcc cctccaggag caggttcacg
      721 gcggtcgggg agtagatgca cccttgcgag aggcacgccc ggacgctttc gaatacgcct
      781 ttccgggtgg tctggtcgcc catattcccc agcacgagcg gcgtcgggct gaggtgcccg
      841 aaagccatcg cgccgtagcc gttgcccccc tcgatgaagc gagcaatcgg tagggagttg
      901 acgctcctga gagcggcagc gccgtttccc aagaagaact tgcctcggcc cagcacctcg
      961 tgcgccatgc gaagctgcgt cgcctccgtc acataggcgt tgtcgcactt caggcgaatc
     1021 accttgccct cggcgtccag gtctgccgaa tagccatccc agcggctgta atcatagcgg
     1081 ctgtaaccac gccgggtgaa cgcccagctg aactcatcac agtagatgcc atcggcgcca
     1141 tactggtcga gcgcgcgccg catgccctcc aggatctcct ccaggtaggc gctcccgggg
     1201 cggggcacgt agtagaggac tccccactct ttctccgtca tcgcgcgcac ccacgagcgg
     1261 ctatagccgg catcctcgaa ggtggcgccg cctgccccct ggatcgcgtc cgaggcaatc
     1321 ggccagcgat gctgcaacgg cttgtagatc acctccattg cggggtggat catctgcatg
     1381 atgcgcacgt tggggcacgc acgccggatc tgggcgacct ccttgcccaa ctgctcccag
     1441 tagggctcgc gatgcgcgcg cgacaggaac gcttccacat caaaatccgg gcaggcgccc
     1501 ggtgcgcgcg gcgccccctc gggaaggcga gggtacttgc cggcggcaac gacgcgggca
     1561 tccggctcta gccgctgcca ggggcccaac acgactgtga tggggcccag atgcccgagg
     1621 gcgcgggccg ccctggcctc gggcgttccc tcaccgccag cccgagcata gccccaga
//
LOCUS       DAOSCV010000991         1673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48528_length_1673_cov_4.725587, whole genome shotgun sequence.
ACCESSION   DAOSCV010000991 DAOSCV010000000
VERSION     DAOSCV010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48528_length_1673_cov_4.725587"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PLY56_18380"
     CDS             complement(<1..701)
                     /locus_tag="PLY56_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23497.1"
                     /translation="MSRREQSRLVDALPDFVSAILGKVASLAGFALAVILVYFLYALF
                     SGYVAAYPKLAHQAPAQAAKIVGNLQVASMILVAAAVFVVAYTLLYLWEFDKLAPILA
                     LIGVALHFGAPFGLAQALGPAMHGNAAATLLLGTLTRVGQVLLWAAAIHGIPTLLKQL
                     HTGLAGYGVDSVTRRAARKQPGIVHPLSPCWKLPYCRPFIRERCPRYRESATCWRKKR
                     GCLCDDDIITAALMGG"
     gene            complement(941..1507)
                     /gene="efp"
                     /locus_tag="PLY56_18385"
     CDS             complement(941..1507)
                     /gene="efp"
                     /locus_tag="PLY56_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ23498.1"
                     /translation="MIDTSDFRTGLSIQIDNEPYTLIYFQHVKPGKGGAFVRTKLRSV
                     RTGNVFERTFRAGEKFEPAYLERAEMEYIYRAGDDYFFMNTETYEQTSFTEAQLGDSR
                     KYLKEGMKVSVLIHNGETIGVEIPDTVELEVVETDPGLRGDTASGGSKPATLETGAVV
                     QVPLFIGVGEIIRVDTRTDSYLERVKKS"
BASE COUNT          339 a          528 c          521 g          285 t
ORIGIN      
        1 ccacccatca gcgcggcggt gatgatatca tcatcacaga ggcaaccgcg cttcttcctc
       61 cagcaagtgg cactctcacg atagcgcggg cagcgctcgc ggatgaaggg ccggcagtag
      121 ggaagcttcc aacaggggct gagggggtgg acaatacccg gctgcttgcg cgccgcgcgc
      181 cgcgtgacgc tatcgacccc gtaaccggcc agaccggtgt gcaactgctt caggagcgtg
      241 gggatgccat ggatggcagc cgcccacagc aacacctggc ccacgcgcgt cagcgtcccc
      301 aggaggaggg tggcagcagc gttgccgtgc attgcggggc ccagagcctg ggcgagccca
      361 aacggagcgc cgaaatggag cgccaccccg atgagcgcca ggatcggcgc aagcttatcg
      421 aactcccaga ggtagaggag cgtgtaggca acaacgaaaa ctgccgcagc caccaggatc
      481 atggatgcga cctggaggtt gcccacgatc ttcgccgcct gggcaggggc ttgatgggcg
      541 agcttcgggt aggcagccac gtacccagaa aagagcgcgt agaggaaata gaccaggatt
      601 acggccagcg caaagccggc gagggaggca accttgccga ggatagcgga aacgaaatcc
      661 gggagcgcat ccacaagccg ggactgctct cttctcgaca tctaccgccc gctcctttct
      721 attgggcagg cgagcaccgg ggagcatcct ggcacgctca cactctcggg cgagagcctt
      781 gctggagcac cgtttccgac ggggcatgcc tgtctcgggc atctctccgg tcagcacggg
      841 cccggctcac cgccgcgggg ccggggcacg caggaattgt gagtagatgc agaccgcgcc
      901 ggcagcccac gaccctagcg cggtctgcgc gatacctctc tcaggacttc ttgacacgct
      961 ccagatagga gtccgtacgc gtatcgacgc gaatgatctc acccacaccg atgaagaggg
     1021 gcacctggac cacggcgcct gtctcgagcg tcgcgggctt gctgccgccc gatgccgtat
     1081 cgccccgcag gccggggtcg gtttcgacca cttcgagctc caccgtgtcc gggatctcga
     1141 cgccgatcgt ctctccgttg tgaatcaaca cggagacctt cattccctcc ttgaggtact
     1201 tgcgcgagtc gccgagctgc gcttcagtaa agctcgtctg ctcgtaggtt tccgtgttca
     1261 tgaagaaata gtcatcgccg gcccggtaga tgtactccat ctcagcacgc tcgaggtagg
     1321 cgggctcgaa cttctcgccg gcacggaacg tgcgctcaaa aacgttcccg gtgcgtacac
     1381 tccgcagctt ggtgcgtacg aatgcacccc ccttgcccgg cttcacgtgc tggaagtaga
     1441 tcaacgtgta gggctcgtta tcaatctgaa tgctcaaccc agttcggaaa tcactcgtgt
     1501 cgatcaagaa aggctctcct ccgccgccag cgccaagagt ggcgcgttat cctgtgcggc
     1561 actccgtagg cgagacgaat gcaccaaatg gagtgtagca cgtcggcaag aatcttgtca
     1621 agcgcggaat cgcaggaaat gccgcctggc gtcctgggat tccgacataa gga
//
LOCUS       DAOSCV010000992         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48578_length_1672_cov_2.852814, whole genome shotgun sequence.
ACCESSION   DAOSCV010000992 DAOSCV010000000
VERSION     DAOSCV010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48578_length_1672_cov_2.852814"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLY56_18390"
     CDS             <1..518
                     /locus_tag="PLY56_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ23499.1"
                     /translation="ERLGEIRTLAAVIQRAIRAGHKVLTCGNGGSAAEAAHLATELLG
                     RYKGDRPALPAVALNTDGALLTCIGNDFGYDALFTRQVAGLGQAGDVLVLFTTSGNSR
                     NLVDAARVAKAQGLFTAALLGKDGGALRGIADWELLLPFSETARVQEGHLMVIHLLSE
                     LLEEYPSGGSE"
     gene            complement(537..>1672)
                     /locus_tag="PLY56_18395"
     CDS             complement(537..>1672)
                     /locus_tag="PLY56_18395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOJ23500.1"
                     /translation="PAAARIFGYAPEEAIGQNVSILMPEPYRSQHTDFIRRYLATGQA
                     HIIGIGREVIGQRKDGTLFPMDLAVSEVRLADHVHFTGLIRDITERKQAEAALRQAQR
                     QAEEAYHREKQIAQRFQSTLLPREEERVPGYAIAHSYRPALVEADVGGDFYNLIEIDP
                     RRVGVVVGDVGGKGLDAAVLAAQAQHTLMALLLQDPQHPERVLAEARRPITALDPDRI
                     LTVFLGVLERDTGRLRCASAGHEQPLLWHAAERRMEVLPLDGPAFFGIPAGTYSRIDT
                     RLEEGDLLFLYTDGLPDACGDDGLLGLERVSDLVARNADRPPEVILSVLYDSVIAYSH
                     NRLRDDIVLMAIRRERARLEGHTVFVPAPGTPEWLEAPAALKG"
BASE COUNT          275 a          548 c          566 g          283 t
ORIGIN      
        1 gcgagcggct gggcgagata cgtaccctcg cggcggtgat ccagcgggcg atccgcgccg
       61 ggcataaggt gctgacctgc gggaacggag gcagcgcggc ggaggcggcg catctggcga
      121 cggagctgct gggccgctac aaaggcgacc ggccggcact acccgcggtg gcgcttaaca
      181 cggacggcgc cctcctcacc tgcatcggca acgatttcgg ctacgacgct ctctttaccc
      241 ggcaggtcgc gggtctcggc caggcggggg atgttctggt gctcttcacc acgagtggca
      301 actcgcgcaa cctggtagat gcggcgcgcg tggcgaaggc gcagggcctt ttcaccgctg
      361 cgctcctggg taaggatgga ggcgcgctgc gcggcatcgc cgattgggag ctcttgctcc
      421 cgttctcaga gacggcgcgc gtgcaggaag gccatctgat ggtcatccat ctcctctcag
      481 agctactgga ggagtatcca tccggcggca gtgaataggc ttgcgtccca agcgtatcag
      541 cccttgagag ctgccggcgc ctccagccac tccggtgtgc ccggagccgg gacaaacacg
      601 gtgtgcccct ccagccgcgc gcgctccctt cggatcgcca tcaacacgat atcatcgcgc
      661 agccggttgt ggctgtacgc gatcactgag tcgtagagga cgctcaggat cacctccggc
      721 gggcggtccg cgttccgggc cacaaggtcg ctcacccgct ccagtcccaa gagaccatca
      781 tcgccacacg catccggcaa gccgtccgtg tagaggaaga gcaggtcgcc ctcctcaagc
      841 cgcgtatcga tccgggagta ggtgccggct ggaatgccga aaaatgcggg cccatcaagg
      901 ggcaggacct ccattcgcct ctcggcggca tgccacaaga gcggttgctc gtggcctgca
      961 cttgcgcagc ggagccggcc ggtatcgcgt tcgagaacgc caaggaagac ggtgaggatc
     1021 cggtcgggat ccagagcggt gattgggcgg cgcgcctccg cgaggacgcg ctcggggtgc
     1081 tgcgggtcct ggagaagcag tgccatgagc gtatgctgcg cctgggcagc caggaccgcc
     1141 gcgtccagcc ccttaccgcc cacgtcgccc accaccacgc caacccggcg cggatcgatc
     1201 tcgatcaggt tgtagaagtc accgccaaca tcggcctcga cgagcgcggg acgatagctg
     1261 tgcgcgatgg catagcccgg cacccgctct tcctcgcgag ggagcagcgt gctttggaaa
     1321 cgctgggcga tctgcttctc gcggtgatac gcctcttctg cttgccgctg tgcctggcgg
     1381 agcgccgcct cggcctgctt ccgctcggta atgtcgcgaa tcagccccgt gaaatggacg
     1441 tggtccgcga ggcggacttc gctgaccgcc agatccattg ggaagagggt gccatccttg
     1501 cgctgcccga tgacctcccg gccgatgccg atgatgtgtg cctggcccgt ggcaaggtag
     1561 cgccggatga agtcggtatg ctggctgcgg tacggttccg gcatcaggat ggagacgttt
     1621 tgcccaatcg cctcctctgg cgcgtaaccg aagatgcgcg ccgccgccgg gt
//
LOCUS       DAOSCV010000993         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48585_length_1672_cov_2.448361, whole genome shotgun sequence.
ACCESSION   DAOSCV010000993 DAOSCV010000000
VERSION     DAOSCV010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48585_length_1672_cov_2.448361"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1672
                     /locus_tag="PLY56_18400"
     CDS             <1..>1672
                     /locus_tag="PLY56_18400"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOJ23501.1"
                     /translation="SREQCETAEITVLPRKPGPLARAEVRPHRGTPTLFINGRPDTGM
                     NYIHNRDVPFHVKNIGGAGVQLFVVFTGNIGWVGPDQYDYSAADRMILSVLLNYPDAY
                     IIPTFDLSGKAFPFWRQLVPKEEWTVTADGQHNVGDYHGARNEFPSLASPTWARLSQE
                     ALRRFARHLYSSPFADRIVGYWPCDGISWEWFPWGAQSREMVDYSPAYRRAFQEWLRR
                     QYPDLAALNRAWHTRLASFEEVTVPTKEERLATDRLAFRDPAGPMRRVIDFYRFHHEQ
                     VAERIEAYARVLKEESGSRHIVGTYYGYVFHLPESPYFGAHSGHFALNRYLASPYLDF
                     GCSPTPYGNVRAVGAPSAFMTAAESFRLAGKLWIQQADIRTHWTTQPHSPAKNIRESA
                     EILRREFVLSFVKGHLVQWYDFSNGWTSGDPRLCRVVQQLQEIARANLDVDRGSPADT
                     LAVVLDETHIYHQRPDRPLMSGPLTELRYALGQSGVPYDTILASDLTRKDARRYRAYL
                     FANCYRLTEEQRQAVEALKSEGRVLAFVYAPGILSENGYHPERVSQVVG"
BASE COUNT          270 a          594 c          530 g          278 t
ORIGIN      
        1 tcgcgcgagc agtgcgagac cgcggagatc accgtgttgc cgcgaaagcc cggcccgctc
       61 gcgcgcgccg aggtgcgccc ccatcgcggt acgcccacgc ttttcatcaa tggccggcca
      121 gataccggga tgaactacat ccacaaccgc gacgtgccct tccatgtgaa gaacatcggg
      181 ggcgccggcg tgcagctctt cgtcgtcttc acggggaaca tcggctgggt gggaccggac
      241 cagtacgact actctgccgc cgaccggatg atcctctccg tgcttctcaa ctacccggat
      301 gcctacatca tccccacctt tgatctttcg ggcaaggcat ttcccttctg gcggcagctc
      361 gtgccgaaag aggagtggac ggtgaccgcc gacggccagc acaacgtcgg cgactaccac
      421 ggggcgcgca acgagttccc ctcgctggcc tcgcccacct gggcccggct ctcacaggag
      481 gcgttgcgcc ggttcgcgcg ccatctctac tcctcgcctt tcgcggaccg catcgtgggc
      541 tattggccgt gcgatggcat cagctgggag tggttcccct ggggcgcgca gagccgcgag
      601 atggtcgatt actcgccggc ctaccggcgc gcgttccagg aatggcttcg ccgccagtat
      661 cccgacctcg ccgcgctcaa ccgcgcctgg cacacgcggc ttgcctcttt tgaggaggtc
      721 acggtgccaa cgaaggaaga gcgcctcgcc accgaccggc tggcgtttag agacccggcc
      781 gggccgatgc gccgcgtgat cgacttctac cgcttccatc acgagcaggt ggccgagcgg
      841 attgaggcct acgcgcgcgt gcttaaggag gagagcggta gccggcacat cgtcggcacc
      901 tactacggct atgtcttcca cctgcccgag tcgccctact tcggggcgca ctccgggcat
      961 ttcgcgctaa accgctacct ggcgtcgccc tacctcgact tcggctgctc gccgacgccg
     1021 tatgggaatg tgcgcgccgt gggcgctccc agcgcgttca tgaccgcggc cgaatcgttc
     1081 cggctggccg gcaagctctg gatccaacag gcggatatcc gcacgcactg gacgacccag
     1141 ccgcatagcc ccgcgaaaaa catccgcgag tccgccgaga tcctgcgccg cgagttcgtc
     1201 ctctccttcg tgaagggcca cctggtgcag tggtacgact tcagcaacgg gtggacgagt
     1261 ggcgatccgc ggctctgccg cgtggtccag cagctccagg agatcgcccg ggcgaacctg
     1321 gacgtggacc gcggctcgcc ggcggacacg ctcgccgtgg tcctggatga gacgcacatc
     1381 taccaccagc ggcccgaccg cccgctgatg agtggcccgc tcacggagct ccgatatgcc
     1441 ctggggcaga gcggggtgcc ctacgacaca atcctggcgt ccgatctaac gcgcaaggac
     1501 gcgcgccgct accgcgccta cctcttcgcc aactgctacc ggctgacgga ggagcagcgc
     1561 caggccgtgg aagcgctcaa gtccgagggc cgcgtgctcg cctttgtgta cgccccgggc
     1621 atcctgagcg agaacgggta ccacccggag cgcgtctcgc aggtggtcgg cc
//
LOCUS       DAOSCV010000994         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48785_length_1667_cov_2.762407, whole genome shotgun sequence.
ACCESSION   DAOSCV010000994 DAOSCV010000000
VERSION     DAOSCV010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48785_length_1667_cov_2.762407"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLY56_18405"
     CDS             complement(<1..547)
                     /locus_tag="PLY56_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23502.1"
                     /translation="MLRKAIRRGIPGIVGLLLLGAIVSANAAPKMRIVAYINVTSGCQ
                     EETVKRLKAFQAKHSKDVHLEIIDFGSEEGFARWRADGFHCQEILINGSDQFRIGSGP
                     TARVVAFRMPEGVRWTFADLDAVLAQELKAPGSSAITDEEARKLAQRVPISSRQGKWK
                     GQSVGEVVVGAQVVFRYRSTLD"
     gene            complement(563..1249)
                     /locus_tag="PLY56_18410"
     CDS             complement(563..1249)
                     /locus_tag="PLY56_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ23503.1"
                     /translation="METHIQLKRVTRLFQKPGETIRALDGVDLAIPRGSWSVIVGVSG
                     SGKSTLLSLVGGLDRATEGEVLVDGRDLSLLSSDELAEYRRTQVGFVFQSFSLIPTFT
                     ALENVCLPFIPYKSARREAEQKAWEMLEKVGMASRANHLPGEMSGGEQQRVAIARALV
                     NDPALLLADEPTGELDRATGEKVLDLLRHLHEERGVTILMTSHDPSIAEQAPRLIHLE
                     DGRVTACVTR"
     gene            complement(1260..>1667)
                     /locus_tag="PLY56_18415"
     CDS             complement(1260..>1667)
                     /locus_tag="PLY56_18415"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ23504.1"
                     /translation="VFLLVMKSSLASVGERVREIGTMKAVGWRDAHVARLLALESALV
                     GIIGGGVGTATGWGAAWLYASHTRVQLPSLLNSYPACAKTPPPVDMQFSLEGSGPLVI
                     GAFLVALAIGIFSGYLAARRASKLPPAEALRRV"
BASE COUNT          285 a          583 c          518 g          281 t
ORIGIN      
        1 catcgagcgt gctgcggtag cgaaagacaa cctgagcgcc gacgacgact tcgccgaccg
       61 actggccctt ccactttccc tgccgggagg agatcggcac gcgctgcgcc agcttccgtg
      121 cctcctcatc ggtgatcgcc gagctaccag gagccttcag ctcttgcgcg agcacggcat
      181 ccagatcggc gaaggtccag cgcacgccct cgggcatccg aaaggcgacg acacgcgccg
      241 tgggcccact gccgattcgg aactggtcgc tgccgttgat cagaatctcc tggcagtgga
      301 agccgtcggc gcgccagcgc gcgaagcctt cctcgctgcc gaaatcgatg atctccaggt
      361 ggacatcctt gctgtgcttc gcctggaagg ccttcagccg cttgaccgtt tcctcctggc
      421 agccgctcgt cacgttgatg taggcgacaa tgcgcatctt gggcgccgca ttggccgaga
      481 cgatagctcc gaggagcagc aagcccacga tgccagggat cccacggcgt atcgctttcc
      541 gcagcattcc tctctccttc tctcaccgcg tcacgcaggc ggtgacgcgc ccatcttcca
      601 gatggatcag tcgcggcgcc tgttcggcga tgcttgggtc gtggctcgtc atcagaatcg
      661 tcacgccccg ctcctcgtgc aggtggcgca ggagatccag caccttctcg ccagtcgccc
      721 tgtccagctc ccccgtcggc tcatccgcca ggaggagcgc cgggtcgttc actaacgcgc
      781 gggcaatggc aacacgctgc tgctcgcccc cgctcatctc gcccggcagg tggttggcgc
      841 ggctcgccat tcccaccttc tccagcatct cccacgcctt ctgctccgcc tcgcggcggg
      901 cgctcttgta cggaatgaag ggcaggcaga cattctcgag cgccgtgaag gtggggatga
      961 ggctgaacga ctggaagacg aaaccgacct gggtgcgccg gtactcggcc agctcgtccg
     1021 agctgagaag gctcagatcg cgcccgtcca ccagtacctc gccttctgtg gcgcggtcca
     1081 ggcctccgac caggctgagc agggtgctct tgccggagcc agaaacaccg acgatgacgc
     1141 tccagcttcc acgggggatc gccaggtcaa cgccatccag ggcgcggatc gtctcgccag
     1201 gcttctggaa gagccgcgtc actctcttca gttgaatgtg ggtctccatg atcaggtgct
     1261 catacgcgac ggagggcctc ggccggtggg agcttgcttg cccgccgggc ggccagatag
     1321 cccgagaaaa tgccgatggc gagcgccacc aggaaggcgc cgatgaccag ggggcccgac
     1381 ccctccaggg agaactgcat atccaccggg ggaggcgttt tcgcgcatgc ggggtagctg
     1441 ttcagcagcg acggcagctg aacccgggtg tgggatgcgt agagccacgc ggcaccccag
     1501 ccggtggccg tgcccacgcc gcccccgatg atcccgacaa gcgccgattc cagggcgagc
     1561 agccgggcca catgcgcatc acgccagcca acggccttca tcgtgccgat ctcgcgcacc
     1621 cgctcgccca ccgacgcgag agatgacttc atcaccagca ggaagac
//
LOCUS       DAOSCV010000995         1664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48898_length_1664_cov_3.575513, whole genome shotgun sequence.
ACCESSION   DAOSCV010000995 DAOSCV010000000
VERSION     DAOSCV010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48898_length_1664_cov_3.575513"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1664
                     /locus_tag="PLY56_18420"
     CDS             <1..>1664
                     /locus_tag="PLY56_18420"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOJ23505.1"
                     /translation="YEYPIAERGQLELGAKLLWANIEYIGIVTIPTAWWAFAVYYSGQ
                     ENWFTPRRWVGLLVVPAVTLILVWTNDWHGLMRQGVFLDTSGPFSTVGKRYGPWFWLW
                     VAYTYTLLFLSTLLLGRALAGEFHLYRGQSVAMGCAIFFPWFANFLYVMELSPLPKMD
                     PTPIAFGCSGLVWTWALSRYRLMDIVPIARDAVLENMCDGVVVLDTSNYVVDLNPAAR
                     SLLDAEDGAIGRKAEEMLGACHELLGCLESAEEKRLEVRVGEAPKERRCDTLVSPLLD
                     RRGHLRGRLVVLRDITEIRRAQGERRRLDARVQQAQRLESLGVLAAGVAHTFNNLLVG
                     ILGNSNLALMYLPEDSPAREHLQQLEKAAQQAAEITRQMLAYSGKGHFVISHVHLTRL
                     AREMAPLLEAVLGPEVNIRYELDPEAPEIRADEGQMRQILFNLVTNASEALQETGGTI
                     LVSTGAMQVDRSLLSETFPGDRLPEGRYAFLRVTDDGCGMDAAMQTRIFEPFFTTKFA
                     SRGLGLAAVLGIVRGHRGTIRVESQPGKGSSFLILLPSASSQEIPP"
BASE COUNT          268 a          526 c          559 g          311 t
ORIGIN      
        1 ggtacgaata cccgatagcc gagcgaggcc agctcgagct cggcgcgaag ctcctctggg
       61 cgaacatcga gtacatcggg attgtcacta ttccgaccgc ctggtgggct ttcgcggtct
      121 actactcggg tcaagagaac tggttcaccc cgcggcgttg ggtgggcctc ttggtggtgc
      181 ccgccgtgac cctcatcctg gtgtggacga acgactggca cgggctgatg cgccagggtg
      241 tcttcctgga tacctccggg ccgttttcca ccgtcgggaa aaggtacggg ccctggtttt
      301 ggctgtgggt tgcctacacg tacacgctgc tgtttttgag cacgcttctt ctcgggcgag
      361 cccttgcggg ggagtttcat ctctaccgtg gccaatcggt ggcgatgggc tgcgccatct
      421 tctttccctg gtttgccaac ttcctctatg tgatggagtt gagccccctc cccaagatgg
      481 acccgacgcc gatcgcgttt ggatgctcgg ggctggtgtg gacgtgggcg ctctctcgct
      541 accgactgat ggacattgtc cccatcgccc gcgacgcggt cctggagaac atgtgcgatg
      601 gggtcgtggt cctggatacc tcgaactacg tcgtggacct gaacccggcg gcgcgctccc
      661 tcctggatgc cgaggatggt gcgatcgggc ggaaggccga ggagatgctg ggtgcctgcc
      721 atgagctgct cgggtgcctg gagagcgcgg aggagaaacg cctggaggtg cgagtgggtg
      781 aggcgccgaa agagaggcgg tgcgacacgc tggtctcccc gctcctggac cgtcgcgggc
      841 acctgcgcgg gcggctggtc gtactccgcg acatcaccga gatccgccgg gctcaggggg
      901 agcgccggcg cctggatgcg cgggtgcagc aagcgcagcg cctggagagc ctgggggttc
      961 tggcggcggg agtggcgcac accttcaaca acctgctcgt gggcatcctc ggcaactcca
     1021 acctggcgct gatgtacctc ccggaggatt caccggcgcg cgagcacctt cagcagctgg
     1081 agaaggccgc ccaacaggcg gcggagatca cccgccagat gctggcctac tccggcaaag
     1141 gccactttgt catcagccac gtccatctca cgcgcctggc tcgcgagatg gcgcccctgc
     1201 tcgaagcggt cctcggcccg gaggtgaata tccgctatga gctcgatccg gaggcgcccg
     1261 agatccgggc tgatgagggc cagatgcgcc agatcctttt caacctggtc acgaacgcct
     1321 cagaggcact gcaggagacc gggggcacca tcctcgtgag cactggcgcc atgcaggtgg
     1381 ataggagcct gctctcagag accttccccg gcgaccggct tccagaaggc cgctatgcct
     1441 tcctcagagt gaccgacgac ggatgcggca tggatgcggc gatgcagacg cggatcttcg
     1501 agccgttctt taccacgaag ttcgcgagcc gtggcctggg cctggctgcc gttctgggga
     1561 tcgtgcgcgg ccacagaggc accatccgcg ttgagagcca gcccggcaag gggagcagct
     1621 tcctcatcct gctcccctca gcctcctcac aggagatacc ccct
//
LOCUS       DAOSCV010000996         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_48933_length_1663_cov_3.167289, whole genome shotgun sequence.
ACCESSION   DAOSCV010000996 DAOSCV010000000
VERSION     DAOSCV010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48933_length_1663_cov_3.167289"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="PLY56_18425"
     CDS             complement(<1..990)
                     /locus_tag="PLY56_18425"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOJ23506.1"
                     /translation="MSTRRAPTPCYFALLLIAAWVLAGLASVTAQDPPRRYLVRLKES
                     VDLNAARGNRRAVVAQLRQTATRTQAPLLIRLEAWRRQGEVQEYEPLWIVNAIRVQAS
                     ERVIAALRRDPAVASVLPAQPVTLPPAQRTRVVGASRQSDRPSWGLVKIGAPELWERN
                     PAIRGTGVTVAVIDTGITPEHPQFQQPGKIRAWKDFVAGKTAPYDDAEWAHGTHVAGT
                     IAGINADGWVVGIAPDATLLVAKALDAQSGGLIDTAIAAMQWAMDPDGNPNTADGADI
                     VNCSLGGPHLEGHWNPTLKSTLEALRAANILPVFAIGNSGPGPKTTVLPGDYPT"
     gene            1216..>1663
                     /locus_tag="PLY56_18430"
     CDS             1216..>1663
                     /locus_tag="PLY56_18430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ23507.1"
                     /translation="MKLLFAKDYTREELLRVLQPRAAADLAVAEQAVRAICADVRARG
                     DDAVVEYTRRFDSPAITDASRLRVTPEEIAGAAEQVSPAYREAVDAAIRRIERYHQKQ
                     LRPSWFDTSDGVLLGQLIRPIERVGLYIPGGKAPLASTLLMTAIPAR"
BASE COUNT          232 a          550 c          607 g          274 t
ORIGIN      
        1 ggtggggtag tcgccaggga ggaccgtcgt ctttggacct ggcccggagt tcccgatcgc
       61 gaaaacgggg aggatgttcg ccgcgcggag cgcttccagg gtgcttttca gggtagggtt
      121 ccaatggcct tcgaggtgag gcccgcccaa tgagcagttc acaatgtcgg cgccgtccgc
      181 ggtgttgggg ttgccgtccg ggtccattgc ccactgcatg gcggcgatgg ccgtatcgat
      241 caggccgccg ctctgcgcgt cgagcgcttt ggccaccagg agggtggcgt ccggtgcgat
      301 cccgaccacc caaccatccg cgttgatccc tgcgatggtg cccgcgacgt gggttccgtg
      361 ggcccactcg gcgtcgtcat acggcgcggt ctttccggcg acgaaatcct tccaggcgcg
      421 tatcttgccg ggctgctgga actgcgggtg ctcgggcgtg atcccggtgt caatcacagc
      481 cacggtgaca ccggtaccgc gaatggcggg gttgcgctcc cagagctcgg gcgcgccgat
      541 cttcaccagg ccccaggagg gccgatcgct ctgccggctc gccccgacca cgcgcgtgcg
      601 ctgagcaggt ggcagggtga ccggctgcgc cggcaggacc gaggccaccg ccgggtcgcg
      661 gcgcagggcc gcgatgacgc gctcgctcgc ctgaacccgg atcgcgttga cgatccagag
      721 cggctcgtac tcctggacct cgccctgccg ccgccatgct tccagccgga tcagcagggg
      781 cgcctgggtg cgcgtggccg tctgccgcag ttgggccacg acggcacgcc ggttcccccg
      841 ggcggcgttg aggtcgacac tctctttcag gcgcaccaga taccgccgcg gcggatcctg
      901 ggcggtgacg ctcgccaggc ccgccagcac ccaggccgcg atgaggagca gcgcgaagta
      961 gcagggggtg ggcgctcgcc gggtagacaa cgccggtctc cttccatctg acgggccaag
     1021 ccggcgcccg cacaccaagt ccttcgccgt ctcccgcgcg ctctccttgc cgcgcgaaca
     1081 ccggcccgcg cgggcggcgc ggggatgggt tggcattgac agggctggtt ggccgcggct
     1141 ataatggccc ctggtagaca gccacacggt tgaggcggcc tgagaggccg tacgttggag
     1201 atagagggtt atctgatgaa gttgctcttt gcgaaggatt acacgcgcga ggagctgctg
     1261 cgcgtgttgc agccgcgcgc ggcggcggat ctcgcggtgg cagagcaggc ggtgcgcgcg
     1321 atctgcgcgg acgtgcgcgc ccggggcgat gatgccgtgg tcgagtacac ccggcggttc
     1381 gactcgccgg cgatcaccga cgcgtcacgg ctccgggtga cgcccgagga gatcgcggga
     1441 gccgccgagc aggtgtcgcc ggcctaccgc gaggcggtag acgcggcgat ccggcgcatc
     1501 gagcgttacc atcagaagca gcttaggcct tcgtggtttg atacgagtga tggcgtcctc
     1561 ctcgggcagt tgatccgccc gatcgagcgt gtcggcctct acatcccggg tggcaaggcg
     1621 ccgctcgcga gtacgctcct gatgacggcg attcccgcgc gcg
//
LOCUS       DAOSCV010000997         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49016_length_1661_cov_2.902242, whole genome shotgun sequence.
ACCESSION   DAOSCV010000997 DAOSCV010000000
VERSION     DAOSCV010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49016_length_1661_cov_2.902242"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PLY56_18435"
     CDS             complement(<1..1219)
                     /locus_tag="PLY56_18435"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ23508.1"
                     /translation="MNPRRWVVFALLAVVLPAAAAEREITLKVNKQPLSEVVRQISEQ
                     AGVPVRVTAFAHKEIPTLDVEGSLERVLGMVARLAGLTVRQEGRGYVLEPEAKPAAPP
                     PLSELNPNRAADIIASVKPRVALVIAYLSAEQARAQGTAFVAGPDGLLVTNAHVVKGA
                     AAIQVVFSDGTAYYAVTAAMDEDKDLAVLRVPARFAEPLRLGDSDELREGDDVALTGY
                     PLALELLRNGIPLYASTAKATVNAVRPGRSLVHKQSMLYIQIDAPISPGNSGAPLYRL
                     DNGVVVGVVQSKLAYEYVQDTGVGFAIPINFVRRLLAEARANSIAPPATPPVPLTASN
                     VPLALPPPLAEGDSAGGAEGASAKPPLTRPFDLLRITQVPPIPEPARVPGVIALHAPG
                     GKMAVDPQRPRLVL"
     gene            complement(1376..>1661)
                     /locus_tag="PLY56_18440"
     CDS             complement(1376..>1661)
                     /locus_tag="PLY56_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23509.1"
                     /translation="RSVIRPAFAGKNGHGWVAEGLYQFSGRLQAFVNYNSLNRAGSGT
                     AVHTWTLGGRVKVHDRLYLIPEVWLVDDDLPKTDALYDDNRYMLTALALF"
BASE COUNT          288 a          549 c          557 g          267 t
ORIGIN      
        1 cgaggacgag gcgcgggcgc tggggatcaa ccgccatctt cccgccggga gcgtgcaggg
       61 cgatcacgcc gggcacccgc gccggttcgg ggatgggggg cacctgcgtg atccgcagga
      121 gatcgaaggg ccgggtgagc ggtggtttcg ccgaggcacc ctcggcaccg cccgcggaat
      181 ccccctccgc caaaggcggc ggcaacgcca ggggcacgtt cgaggccgtg agcggcacgg
      241 gcggcgtcgc cggcggggcg atggagttgg cgcgcgcctc cgccagcaag cgtcggacga
      301 agttgattgg gatcgcaaag ccgaccccgg tatcctggac atattcgtag gcgagcttcg
      361 actggacgac gccgacgacg acaccgttat ccaggcgata gagaggcgcg ccgctgttgc
      421 ccgggctgat cggcgcgtcg atctggatgt aaagcatcga ctgcttatgc actaggctgc
      481 ggccagggcg cacggcattc actgtcgcct tcgcggtgga agcgtagagg ggaatcccgt
      541 tgcgcaggag ctcaagcgcc agcgggtagc ccgtcagcgc cacatcgtcg ccctcgcgca
      601 gctcatcgga atcgcccagg cgcagtggct cggcaaaccg ggcgggcacg cggagcaccg
      661 ccaggtcctt gtcctcatcc attgcggcgg tcaccgcgta gtacgccgtg ccatccgaaa
      721 agacgacctg gattgccgcg gctcccttga ccacgtgcgc gttggtgacc aggaggccat
      781 ccgggccggc cacgaaagca gttccctggg cacgcgcctg ttctgccgac aggtaggcaa
      841 tgaccagcgc cacgcgcggt ttcacgcttg cgatgatatc agccgcacgg ttggggttca
      901 gctcggaaag gggtggcgga gcagcgggct ttgcttccgg ttcgagaacg tatcccctgc
      961 cctcctgacg cacggtgagc ccggccaaac gcgccaccat gccgaggact ctctccagag
     1021 acccctccac atcgagggtg ggaatctcct tgtgcgcgaa tgcggtcaca cggaccggca
     1081 cgccggcttg ctcggagatc tggcgcacta cctccgagag gggttgtttg ttgaccttga
     1141 gagtgatctc gcgctccgcg gcggccgccg gaagaacgac cgccagaaga gcgaacacca
     1201 cccatcgtct cggattcatg cttgggtcct cggggcactc gcggcgttct cgtctcggac
     1261 attatagcat cggcgcggat ccagcgtcaa ggctggggcc ggggggttgg agaggcatca
     1321 gacccgcacc tctccatccg cacccggccc ctttatccgc ggtatcgccg cccgctcaga
     1381 agagcgccag cgcggtcaac atgtaccggt tatcgtcata gagggcatct gtcttcggca
     1441 gatcatcgtc caccagccac acctcgggaa tcaggtagag ccggtcatgc accttcaccc
     1501 gcccgccgag agtccaggtg tggaccgccg tgccagaccc cgcgcggttg agcgagttgt
     1561 agttcacgaa tgcctgcaag cgcccactga attggtagag accctccgcc acccagccgt
     1621 gcccgttctt cccggcgaag gcggggcgga tcacagatcg g
//
LOCUS       DAOSCV010000998         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49179_length_1657_cov_3.393258, whole genome shotgun sequence.
ACCESSION   DAOSCV010000998 DAOSCV010000000
VERSION     DAOSCV010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49179_length_1657_cov_3.393258"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLY56_18445"
     CDS             complement(<1..609)
                     /locus_tag="PLY56_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23510.1"
                     /translation="MSLLSRISKGGGAPLPDPDRQPMADRLPTPDAPERLPAAGTSQP
                     QATQPPELDGIVWRLMAQVGQVIAPSGVTSRNVERGERDQRMYAIEEHALPILQQEGI
                     SLTPTERTKVFSRLYDELFGLGPLESLLDDSTVRRIVVNGPREVLAERYSGLEKTDAA
                     FKDDAHLVLIVRRIASLVEVPLDDSIPRMSATLQDGARVQAVL"
     gene            complement(754..>1657)
                     /locus_tag="PLY56_18450"
     CDS             complement(754..>1657)
                     /locus_tag="PLY56_18450"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ23511.1"
                     /translation="EVVTATQRVWDKERRRKQRERGLTPRSPVDSKIITFYSPHDGVG
                     KTTLSANMAVALALDTGDPVALVDLNLQFGDLDLVLNLAPQRTIVDALPQFQHLDASM
                     IETFLTPHESGVRLLAAPPRPQYAETVTVYCVQQVLRVLKESYRYVIVDAASVLNDTT
                     LAALDLSDEILLITNLDVPSLRNAKICLETMHDLHYETEKIKLVINRATGEMGIKADE
                     VEEYLEHPVAARIPSEGRTVVASVNQGTPFVISNPEAKVSEAIHQLAYLFAPEFEAVR
                     APAEAPRKGFGMLLRQLLGTGTSS"
BASE COUNT          292 a          514 c          542 g          309 t
ORIGIN      
        1 cagcacggcc tgcacccggg cgccatcctg aagcgtggcc gacatgcgcg ggatgctatc
       61 atcgagcggg acctccacca gactggcgat acggcgaacg atgagcacca gatgggcgtc
      121 atccttgaag gcggcatcgg ttttctccag gccgctgtaa cgctcggcga gcacctcgcg
      181 aggcccattg acaacgatgc gcctgaccgt ggagtcgtcc aggagcgatt cgagcgggcc
      241 gaggccgaac agctcgtcgt agaggcgcga gaagaccttg gtgcgttctg tcggggtgag
      301 gctaatccct tcctgctgta ggatggggag cgcgtgctcc tcgatcgcgt acatccgctg
      361 gtcgcgctca ccgcgctcca cattgcgtga agtcactccc gacggcgcga ttacctggcc
      421 aacctgcgcc atcaatcgcc agacgatgcc atcgagctcc gggggctggg ttgcctgagg
      481 ttgcgaggtg ccggcagcag gcaagcgttc gggcgcgtcg ggggtgggca atcgatccgc
      541 catgggctgc cggtctggat ccggcagcgg ggcacctcct cctttgctga tgcgggaaag
      601 cagcgacata acagctccgc ggcccgccag tacccaggcg cgtcgcaagc ttccggagcg
      661 cctccggccg gcgaggccag ctcatcccct ttccgaataa cgcttcaaac cacgtgaatg
      721 cgggttctga ataagctcta cgccaccgtg gggttagctt gaggtgccag tgcctagcag
      781 ctggcgcaaa agcatgccaa agcccttgcg cggcgcttcg gcaggtgccc gcaccgcttc
      841 gaactccggc gcgaagagat aggccagttg gtgaattgcc tcgctcacct ttgcctctgg
      901 gttcgatatc acaaagggag ttccttgatt tacggacgcc acgacggtcc gcccctcgct
      961 agggatacgg gcggccaccg ggtgttcaag atactcctcc acttcgtctg ccttgatccc
     1021 catctcgcct gttgcacggt taatcaccag cttgatcttc tccgtctcgt agtgcaggtc
     1081 gtgcatcgtc tcgaggcaga tcttggcgtt gcgcaacgag ggcacgtcga gattggtgat
     1141 gagcaggatc tcatccgaga ggtcgagcgc cgccagggtg gtgtcgttca gcacggaggc
     1201 agcatccacg atgacgtagc gatagctctc cttgaggacg cggagcacct gctgcacgca
     1261 gtagacggtc accgtctcgg catactgggg ccgcggcgga gcggcgagaa gccggacgcc
     1321 ggactcgtgc ggtgtcagaa aagtctcaat catgctcgcg tccaggtgct ggaactgggg
     1381 cagcgcatcc acgatggtcc gctgcggggc cagattcagg acgaggtcca gatctccaaa
     1441 ctgcaggttg agatcgacga gagctaccgg gtctcccgta tcgagcgcca gcgccaccgc
     1501 catgttggcc gagagcgtgg tcttgcccac gccatcatgc gggctgtaga aggtgatgat
     1561 cttggagtct acgggggaac gcggggtgag gccgcgctcg cgctgcttgc gccggcgctc
     1621 tttgtcccac acgcgttggg tggcggtcac cacctcg
//
LOCUS       DAOSCV010000999         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49436_length_1651_cov_2.915414, whole genome shotgun sequence.
ACCESSION   DAOSCV010000999 DAOSCV010000000
VERSION     DAOSCV010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49436_length_1651_cov_2.915414"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1651)
                     /locus_tag="PLY56_18455"
     CDS             complement(<1..>1651)
                     /locus_tag="PLY56_18455"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HOJ23512.1"
                     /translation="VGYFIRLYQNGEQVASREVLQCTSATTDNLVDISKGFGGPWFLN
                     GEIAHLTVYNRALSSTELNALLRAEKLAKVRFKPVAASDPRYPAFLARAREAVMAQPD
                     ARRRPLARLLAAVEQANIHIETQAALLPYLETVRRLASSGQDPIRQFAAAHREFTFLG
                     SERLSLALFTPRQGRTVLCSLYDLRAGREVLGEQRALWTLHYETATGGPLHEIDSTAD
                     SLAVKLTVDPSGKAARLEWTHPGSAEHPIQFTVRSELSLQGPRLALNFSLDNRSPRIA
                     VRQARFPIIRLRRLEDGTDRLLVPRMSGVVHENPVRTYFKYEGPYPSGAAHMQFFAYY
                     DDRSGVYVACEDGKARSKDLRAFADDNDCDLSYLWHVGCQGAGGNDFTANGVAAIEVF
                     EGDWFDAAQIYKRFARTAGWWPASRTREDIPAWYREMTVWFLGNANTQAAIDSLLAMR
                     EELGLPIGLHWYNWNKEAFDDDYPHFTPREGFAETVARLQAKGVYVKPYINARLWETR
                     DRGDEDFEYTSVALPATVKDRQGKPATETYAKRTFSPMCPAT"
BASE COUNT          266 a          539 c          563 g          283 t
ORIGIN      
        1 ttgtcgccgg gcacatcggc gagaaagtgc gcttggcata ggtctcggtc gcaggcttcc
       61 cctggcgatc cttcaccgtc gccggcagcg ccacagaggt gtattcgaag tcctcatcgc
      121 ctcggtcgcg cgtctcccag agccgggcgt tgatgtaggg cttgacataa accccttttg
      181 cctggagccg ggccacggtt tcggcaaagc cctcacgcgg cgtgaagtgg gggtagtcgt
      241 cgtcaaacgc ctccttattc cagttgtacc agtgcaggcc aatcggcagg ccgagttcct
      301 cgcgcatcgc gagcagcgag tcgattgccg cctgggtgtt ggcattcccg aggaaccaga
      361 cggtcatctc gcggtaccag gcgggaatgt cctcgcgcgt ccgggaggcc ggccaccacc
      421 ccgctgtgcg cgcgaagcgc ttgtagatct gcgctgcgtc gaaccagtcg ccctcgaaca
      481 cctcgatcgc cgccacgccg tttgcggtaa agtcgttgcc gccggcgccc tggcagccga
      541 cgtgccacag gtaggagagg tcgcagtcgt tgtcatcggc gaacgcgcgc aagtccttgc
      601 tccgcgcttt gccatcctcg cacgcgacgt acactccgct acggtcatcg tagtaggcga
      661 agaactgcat gtgcgcggcc ccggaggggt acgggccctc atacttgaag taggtgcgca
      721 ctgggttctc gtggaccacg cctgacatgc gcggcaccag caggcggtcg gtgccatcct
      781 ccaggcgccg gaggcggatg attgggaaac gagcctggcg cacggcgatc cgtgggctcc
      841 ggttgtccag tgagaagttg agcgcgagcc gcgggccctg aagcgagagc tcggagcgca
      901 cggtgaactg gatcggatgc tccgcgctgc ccgggtgcgt ccactccaga cgcgcggcct
      961 tgccggacgg gtccacggtg agtttcaccg cgagcgaatc agcggtgctg tcgatctcgt
     1021 gcaacgggcc gcccgtggcg gtttcgtagt ggagcgtcca gagcgcgcgc tgctcgccaa
     1081 ggacctcccg cccggcgcgc agatcataga ggctgcaaag cacggtccgg ccctggcgcg
     1141 gcgtaaagag cgcgagtgag agccgctcgc tgcccaggaa agtgaactcg cgatgcgccg
     1201 cggcaaactg ccggatcggg tcctgaccag aggacgccag gcggcgcacc gtctccaggt
     1261 agggcaacag cgccgcctgg gtttcgatat ggatgttcgc ctgctccacc gcggcgagaa
     1321 gccgcgcgag agggcgccgg cgcgcgtccg gctgggccat cacggcctct cgcgccctgg
     1381 ctaggaacgc ggggtagcgc gggtcgcttg ccgccaccgg cttgaaccgg accttcgcca
     1441 gcttctccgc gcgcaacagc gcgttcagct cggtgctgga gagcgcgcgg ttgtagaccg
     1501 tgaggtgggc tatctcgccg ttcagaaacc acggcccgcc aaaccccttc gagatgtcca
     1561 ccaggttgtc ggttgtggcg ctcgtgcact gaaggacttc ccgcgatgcc acctgctcgc
     1621 cgttctggta gaggcggatg aagtagccga c
//
LOCUS       DAOSCV010001000         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49471_length_1650_cov_3.979937, whole genome shotgun sequence.
ACCESSION   DAOSCV010001000 DAOSCV010000000
VERSION     DAOSCV010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49471_length_1650_cov_3.979937"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLY56_18460"
     CDS             complement(<1..1087)
                     /locus_tag="PLY56_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23513.1"
                     /translation="MRTLISLFTLLFACSAAGAVPSIVEPVSVGVTVVRDDDGQWRSD
                     LSNAMTHQIKPEYQAKKVLDLSGVPEALWQRVKKVRLSACLLVRDYSWRENPPANGLD
                     EAFEIVINGKVHHFRTNCGVPVYPEGKLPVMAWHDFVVPKEEFTRGVNEIVFRKAAVE
                     KKEYDDYLYLAIDNSVRRGNSSVTFDGQVWMEEKLTIPGGNGEYMVRLYLIEKETQVR
                     VDWAPGTKSRLEDPARLIGYAGSPAGEPTDSGLRLAPGESARIEWNPASLDTLEPVNA
                     RITATGGVQTAWLDPAGEPVEKATGAKALERVLPGGRAFRPSGLVVTAADGPVVLQSV
                     RLDASESYHPKKRPIDMAPKIADPAGKQ"
     gene            complement(1304..>1650)
                     /locus_tag="PLY56_18465"
     CDS             complement(1304..>1650)
                     /locus_tag="PLY56_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ23514.1"
                     /translation="HPGGSLAYRLDWPDRSLAYVTDTTASLDYAEFVRGVDLLIHECA
                     FPDRMEARARETGHSWTSAVSELALAAGAKRLILTHTNPLDPRPDPVELDAARVCFPG
                     VVVAHDGMAVVV"
BASE COUNT          284 a          550 c          523 g          293 t
ORIGIN      
        1 cctgcttccc cgccggatcc gcgatcttcg gggccatatc aatcgggcgc ttcttcgggt
       61 gatacgattc ggaagcatcc agacggacgc tttgaagcac gaccggccca tcggcggccg
      121 ttaccacgag gcccgagggt cggaacgcgc gccctcccgg gagaacacgc tccagggcct
      181 tagcacccgt ggctttctcg accggctcgc ccgcggggtc gagccaggcg gtctgcaccc
      241 ctcccgtagc ggtgatacgc gcattcaccg gctcgagcgt gtcgagcgag gcggggttcc
      301 attcgatgcg cgcgctctcg ccaggggcca gtcgcaggcc gctatcggtt ggctcaccag
      361 cgggagaacc ggcatacccg atcaggcgcg cgggatcctc caggcgcgac ttggtgccgg
      421 gcgcccaatc gactctgacc tgtgtctcct tctcgatcag atagaggcga accatgtact
      481 cgccattgcc gcccggaatg gtgagcttct cttccatcca cacctgtcca tcaaaggtga
      541 cggagctgtt gccacgccgg acgctgttat cgatcgccag gtagaggtag tcgtcatatt
      601 ctttcttctc gaccgccgcc ttgcggaaca cgatctcgtt gacgccccgg gtgaactcct
      661 ccttgggcac cacgaaatcg tgccacgcca tcacgggcaa cttgccctcg ggatagacag
      721 gcacgccaca gttggtacgg aaatggtgga ccttgccgtt gatgacgatt tcaaacgcct
      781 cgtcgaggcc attggcgggc gggttctcgc gccacgagta gtcgcgcacc aggaggcagg
      841 cggaaagccg caccttcttg acgcgctgcc agagcgcctc gggcacgccg gatagatcca
      901 gcaccttctt cgcctggtat tccggcttga tctggtgcgt catggcgttg ctgaggtcag
      961 accgccactg gccgtcatcg tcacgcacca cggtcacccc gacgcttacc ggctcgacga
     1021 tggagggcac tgcccctgcc gctgaacacg cgaagaggag agtgaacagt gatatcagcg
     1081 ttcgcatggt accgctttcc cttccctgga aggcgtcgca tcccatcagg ctggcccgaa
     1141 aggcctcctg aagagtgtca cgtgctggag agatccgggg tggtaggcaa cccgctctcg
     1201 gaccgccggc cactttggcg cgcgccggca gcaagccacg cctcccatta taggtgccct
     1261 accctgcgcc gtcaaccccg gtttcgatgg tgcaatggcc ccctcatacc accacagcca
     1321 tcccatcgtg agccaccaca acgccgggga agcagacgcg ggctgcatcc agctccacgg
     1381 ggtcaggccg gggatcgagt gggttggtgt gggtcaggat gaggcgcttt gccccggcgg
     1441 ccagggcaag ctccgagacg gcgcttgtcc aggagtggcc cgtctctcgc gcccgcgcct
     1501 ccatccgatc tgggaaggcg cactcgtgga tcaggagatc gacgccgcgc acgaactcgg
     1561 cgtagtcgag cgaggcggtg gtatcggtca cgtaggccag ggagcggtcg ggccagtcga
     1621 gacgataggc cagcgatcct cccgggtggg
//
LOCUS       DAOSCV010001001         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49677_length_1645_cov_3.329560, whole genome shotgun sequence.
ACCESSION   DAOSCV010001001 DAOSCV010000000
VERSION     DAOSCV010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49677_length_1645_cov_3.329560"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PLY56_18470"
     CDS             complement(<1..1171)
                     /locus_tag="PLY56_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ23515.1"
                     /translation="MSLDELCVNTIRMISVDAIQKANSGHPGLPMGAAPMAYTLWTRH
                     LKHNPRDPKWPNRDRFVLSGGHGSMLLYSLLYLTGYDLSLDDLKTFRQWGSKTAGHPE
                     YGLTPGVEVTTGPLGQGLATAVGLAMAEAHLAARYNRPGQAVVDHFTYVLATDGDMME
                     GVAAEAASLAGHLRLGKLICLYDSNDISLDGGTPLCFTEDVAKRFEAYGWHVVTVEDG
                     NDVDAIDAAIQEAKANTEQPSLLVIRTQIGYGSPNKQGSSKAHGSPLGKDEVVATRRR
                     LGWPEDVDFHVPQEALAAFRKAVDEGARAQAEWEARFRAWAEANPELAQEWSVAFSGK
                     LPEGWDQAIPVFGPEDSAATRATGGKVLNAIAAHYPGLFGGSADLTGSNLTNWSGC"
     gene            complement(1457..>1645)
                     /locus_tag="PLY56_18475"
     CDS             complement(1457..>1645)
                     /locus_tag="PLY56_18475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23516.1"
                     /translation="IGSQNMSPGELGRYECPNCGFEYDVVLGTAARCPNCNYQADLTN
                     VVGDESFHESFSATYPDD"
BASE COUNT          295 a          524 c          546 g          280 t
ORIGIN      
        1 tgcagcccga ccagttggtc aggttggaac cggtcaggtc ggcagagccg ccgaacaatc
       61 ccgggtagtg cgccgcgatg gcgttgagca ccttgccgcc cgtcgcgcgc gtagcagcag
      121 agtcttcggg gccaaagacc gggatcgctt gatcccatcc ttccggaagc ttcccggaga
      181 aggccacgct ccactcctgc gcgagctcag ggttggcctc ggcccaggcg cggaagcgag
      241 cttcccactc ggcctgtgcg cgggccccct cgtcaaccgc cttgcggaac gcggcgagtg
      301 cctcctgcgg cacgtggaaa tcgacatctt ccggccagcc caatcgccgc cgggtcgcca
      361 cgacctcgtc cttgccaagc ggcgagccat gtgccttgga cgagccctgc ttgttcggag
      421 agccatagcc gatctgagtt cggatcacga ggagcgacgg ctgctcggtg ttcgctttcg
      481 cctcctggat cgccgcgtcg attgcgtcga catcattgcc gtcttccacg gtgaccacgt
      541 gccagccgta ggcctcgaaa cgcttggcca catcctcggt aaagcagaga ggggtgccgc
      601 catcaaggga gatgtcattg ctgtcgtaaa ggcagatcag cttgcccagg cgaaggtgac
      661 cggcgagcga cgcggcttcg gcagccacgc cctccatcat gtcgccatcc gtcgcgagca
      721 cgtaggtgaa gtgatccaca accgcctgtc cggggcgatt gtagcgcgcg gccagatgcg
      781 cctcggccat cgccaggccg accgcggtgg ccaggccctg ccccagcggc ccagtagtga
      841 cctcgacgcc gggcgtgagt ccatactccg ggtgtccggc cgtcttgctg ccccattgcc
      901 ggaacgtctt gagatcgtcc agcgagaggt catacccggt gaggtagagc agagagtaga
      961 gcagcatcga gccgtgaccg ccagagagaa cgaagcggtc acggttcggc cacttcgggt
     1021 cgcgcgggtt atgcttcaga tggcgtgtcc agagcgtgta cgccatgggc gccgccccca
     1081 tcgggaggcc cgggtgcccg gagttggcct tctgaatagc atccacggag atcatgcgta
     1141 tggtgttgac acaaagctcg tcaagcgaca tgggccatct tccccaggta agagatgtag
     1201 acggcaagac cgccgcccca atcactgttc gccgcgagcc cgctggaacc ttctccgaga
     1261 ggcgaagagc gaagcgacgg gggagtagtg gagacgggtg gagcacgggg gatgatggca
     1321 tcgcggtggg gcctcgggaa cggcacgtgc cgtcaccccg cctgcgtctc tccgaccgtg
     1381 tcagcccgga gcagagcgaa gggctttcca cggagaaggc ggaacgctgc agcctgtcgg
     1441 gcgtgcgcct ctcttgtcag tcgtcagggt aggtggcgct aaacgattcg tggaacgact
     1501 cgtcaccgac tacgttggtc aggtccgcct gatagttgca gttagggcag cgcgcggcgg
     1561 tacccaggac gacatcatac tcgaagccgc agttggggca ctcatagcgg cccaactcac
     1621 cgggcgacat gttctgagag ccgat
//
LOCUS       DAOSCV010001002         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_49758_length_1643_cov_3.477330, whole genome shotgun sequence.
ACCESSION   DAOSCV010001002 DAOSCV010000000
VERSION     DAOSCV010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49758_length_1643_cov_3.477330"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLY56_18480"
     CDS             <1..758
                     /locus_tag="PLY56_18480"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ23517.1"
                     /translation="DSVHKVEAGSTQIAEAVAEHAIALLFAIVRQTSRLDRVVREGRW
                     GGGGTRVPGLLGGGVLGLVGFGHIGRHVARKLRGFEMRILVSDPAVPAEEMARYGVEP
                     ATLEEILPQVDFLSLHCPLIPATRGLIGEAQLRQMKPTAYLVNTTRGGVVDEAALVRA
                     LREGWIAGAALDVLEQEPPPPNHPLFALENVVITPHMAASSVRFEQEFWAHSVRALAA
                     LARREWPESWVNHGVKPRWPELAGAPASRQAAR"
     gene            786..1421
                     /locus_tag="PLY56_18485"
     CDS             786..1421
                     /locus_tag="PLY56_18485"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HOJ23518.1"
                     /translation="MKMLEEWLRRLAALPTAALSDVMRRRGMDPCTMHSSMKPVWEGA
                     EVCGPAFTARTYPGATHASGLALSQARPGDVIVLAGGGYTEAILWGEIFSTAAKQLGF
                     GGTVIDGAARDIAGIRELGYPVFARAVTPRGGTGDSQQSETQIPIQCGGVLVHPGDLV
                     RGDSDGVVVIPAALAPEIIAETEAFHAREERILEFLRAGLPLGEACRRAAE"
     gene            1461..>1643
                     /locus_tag="PLY56_18490"
     CDS             1461..>1643
                     /locus_tag="PLY56_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23519.1"
                     /translation="MTPVRPAACHSSSRPSPRCVFFDAFGTLVTLHRPFERLKANLES
                     LGYSIPLGDVEAAMRQE"
BASE COUNT          239 a          550 c          594 g          260 t
ORIGIN      
        1 acgacagcgt gcacaaggtc gaggctggtt cgacccagat cgccgaggcg gtcgcggagc
       61 atgccatcgc gctcctcttc gcgattgtcc ggcagacttc caggctcgac cgcgtggtcc
      121 gcgagggccg gtggggcggg ggcggcacac gtgtgcccgg gctcctggga ggcggagtcc
      181 tcgggctcgt cggcttcggg cacatcggcc gacacgtcgc gcggaagctc cgcggtttcg
      241 aaatgcggat cctggtgagc gatccggcgg tgccagcgga agagatggcg cgctatggcg
      301 tcgagccagc aaccctggag gagatcctgc cccaggtcga tttcctctcg cttcactgcc
      361 cgctgatccc ggcgacgcgc ggcctcatcg gcgaggcgca actgcgccag atgaagccga
      421 ccgcctacct ggtcaacacc acgcgcggcg gggtggtaga cgaggccgcg ctcgtgcgcg
      481 cgctgcgcga gggatggatc gccggggcgg cgctggacgt gctggagcag gagccaccgc
      541 ctcccaatca cccgttgttc gccctggaga acgttgtgat caccccgcac atggccgcgt
      601 cttccgtccg attcgagcaa gagttctggg cgcactcggt gcgcgcgctg gcggcgctcg
      661 cccggcgcga gtggccagaa tcgtgggtga atcacggtgt gaagccccgc tggcccgaat
      721 tggccggtgc ccctgcctcc cggcaggcag cgcgttgagc cgacctgctg gctgagtgga
      781 aaggaatgaa gatgcttgag gaatggctac gccgtctggc cgcgctgccg acggcggcgt
      841 tgagcgatgt gatgcggcgc cgggggatgg acccctgcac gatgcactcc tcgatgaagc
      901 ctgtttggga aggcgccgag gtgtgtggcc ccgcgttcac cgcgcgcacc tatccgggag
      961 cgactcacgc cagtggcctg gcgctctcgc aggcccgccc gggcgatgtg atcgtgctcg
     1021 cgggaggcgg ctacaccgag gcgatcctct ggggcgagat cttcagcact gccgccaaac
     1081 agctgggctt cggtggcacc gtcatcgatg gcgccgcgcg cgacatcgcc ggcatccggg
     1141 agctgggcta ccccgtcttt gcgcgcgccg taacgccccg cggcggcacg ggcgattccc
     1201 agcagtccga gacgcagatc ccgatccagt gcggcggcgt cctggtccac ccgggcgacc
     1261 tggtgcgcgg cgatagcgac ggcgtcgtgg tgatccccgc ggcgctcgcc cccgagatca
     1321 tcgccgaaac ggaagcgttc cacgcccgcg aggagcggat cttggagttc ctccgtgcgg
     1381 gcctcccgct gggcgaggcg tgtcgccggg cggctgaata ggggagaggg gatcgcgtgc
     1441 tggagggagg aaaccctggc gtgacacccg tccggccagc ggcctgtcat tcgtccagca
     1501 ggccctcccc tcggtgcgtc ttctttgacg cctttggcac gctggtcacg ctccaccgcc
     1561 cgttcgagcg cctgaaggcg aacctggaat cgctcggata ctccatcccg ctgggcgacg
     1621 tggaagcggc aatgcgccag gag
//
LOCUS       DAOSCV010001003         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50018_length_1638_cov_2.306380, whole genome shotgun sequence.
ACCESSION   DAOSCV010001003 DAOSCV010000000
VERSION     DAOSCV010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50018_length_1638_cov_2.306380"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..>1638)
                     /locus_tag="PLY56_18495"
     CDS             complement(347..>1638)
                     /locus_tag="PLY56_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ23520.1"
                     /translation="PTLFRMLSSLGKRLYFPKGILAQSAEAKEKANRYNATIGIAREN
                     GKPMALPAVMKHFTDLTPSEALGYAPPMGIPELRNAWREELLRKNPSLKGKSFSNPIV
                     TCGVTHALSLVGDLFLDPGDLVLLPDKFWENYELIFGVRQQAQIASYPFFNEAGGFNI
                     EGLCQVLLTRKDNGTTLVVLNFPNNPTGYSIRKEEADALVEGLVGAADAGCKLVVVTD
                     DAYFGLFYNEDALQESIFARLAGAHERIVAIKVDGPTKEDYVWGFRAGMLTFSASAKT
                     SDEVLYRALERKVAGAIRSTLSSGAMPSQSILAKAMADPDFLAQCSEKRSILEARARK
                     VQEILADPGFADLWEPYPFNAGYFMCLRLKGLDAERYRKHLLEKYGVGVIADGKSDIR
                     VAFASVEIDQLPDLYATLAAAARDLKAEDARTTTRDA"
BASE COUNT          272 a          517 c          558 g          291 t
ORIGIN      
        1 cgccgggttg cgcgctgcat ccgaggcttc ggattcctta tctcggaatc tcagaggagt
       61 gcaactgtct ccgcacgatg cgggtgtgtc acccaccgct cgcgcgcatc gataacgcgg
      121 atgaaggggt cggacacgga gggggatggc gcggcagccc ggcgcccggg ggcgtggttt
      181 gcgtccgggt gatgcatgcg ccccctgtga cggcgtcggc acccggaatg aaggcgacgg
      241 acgcggatgg agaggagcgg agaccctggg ttgcgcgaca gcgggggggg gtacagagaa
      301 agcgccggcc ccccaacggg gggccggcgc accgaacaga ccgctcctat gcgtcgcgcg
      361 tggtagttcg cgcgtcttct gccttcaaat cccgggccgc ggcggccagc gtggcgtaga
      421 gatccgggag ttggtcgatc tccaccgaag cgaacgcgac gcggatatcc gacttgccgt
      481 cggcgatcac gcccacgccg tacttctcca ggaggtgctt ccggtacctc tccgcatcca
      541 ggcccttgag acgcaggcac atgaagtagc cggcgttgaa gggatacggc tcccacagat
      601 cggcgaagcc gggatcggcg aggatctcct gcaccttcct cgcacgtgcc tcgagaatcg
      661 agcgcttctc actgcactgt gccaggaaat cgggatccgc catcgccttc gccaggatgc
      721 tttgcgaggg catggcgccg ctggagagcg tgctgcggat ggcgcctgcg actttccgct
      781 ccagcgcccg gtacagaacc tcgtcgctcg tcttcgcgct cgcgctgaag gtgagcatgc
      841 cggcgcggaa gccccatacg tagtcttcct tggtcgggcc atcaaccttg atcgccacga
      901 tgcgctcgtg cgcgccagcc agccgggcaa agatcgactc ttgcagcgcg tcctcattgt
      961 agaagaggcc gaagtaggcg tcatcggtga cgaccaccag cttgcacccg gcatccgcgg
     1021 ccccgacgag tccctcgacg agcgcgtccg cctcctcctt gcggatggag tagccggtgg
     1081 ggttgttcgg gaagttgagg acgaccagcg tcgttccgtt gtccttgcgc gtcaggagta
     1141 cctggcacaa cccctcgatg ttgaagccgc ccgcctcgtt gaagaagggg tagctggcga
     1201 tctgcgcctg ctggcgcacg ccgaagatca gctcgtagtt ctcccagaac ttgtcgggca
     1261 ggaggaccag atcgcccgga tcgaggaaga ggtcgcccac caggctcagg gcatgggtga
     1321 cgccgcaggt gacgatcggg ttgctgaagc tcttgccctt caggctcggg ttcttgcgca
     1381 gcagctcctc gcgccaggcg ttgcgcagct cggggatccc catcggaggg gcatagccga
     1441 gggcctccga aggcgtgaga tccgtgaagt gcttcatcac cgcgggaagc gccatcggct
     1501 tgccattctc gcgcgcgatg ccgatcgtgg cattgtagcg attggccttc tcctttgcct
     1561 ccgcgctctg cgccaggatc cctttgggga agtaaaggcg cttgcccagc gaggagagca
     1621 tcctgaagag ggtcgggt
//
LOCUS       DAOSCV010001004         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50091_length_1636_cov_3.578748, whole genome shotgun sequence.
ACCESSION   DAOSCV010001004 DAOSCV010000000
VERSION     DAOSCV010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50091_length_1636_cov_3.578748"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..618)
                     /locus_tag="PLY56_18500"
     CDS             complement(175..618)
                     /locus_tag="PLY56_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ23521.1"
                     /translation="MALTRWSPFTEMSRLRDEMERMMDRFFELPERRAEFGATMWVPS
                     IDVYDRDGNVVVEAELPGMKREEVEVTLEDSTLTISGEMKREAEKKEEGYYRSERRYG
                     RFMRSIPLPTPVKANEVKAKFEDGVLKVTLPKAEEAAHGKRVPVE"
     gene            complement(869..>1636)
                     /locus_tag="PLY56_18505"
     CDS             complement(869..>1636)
                     /locus_tag="PLY56_18505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23522.1"
                     /translation="SAGVLFMYAENWIYAPAIQKALRLAEAAGGTIFDIRAEEAHSGS
                     HSPYAREWRTSGGGSLLRLGSHPLAAALYFKRVEGERRDGKPIRVASVSCETTNVTRI
                     PSFEREERKWVASGWKDVEDWATLILNFEDGARATISASDIVLGGIRNRMELYLSNAR
                     IDCNLNPNTTCIAYAPAPEVFADEYIAEKIDTKAGWSFPSINEDWFQGYPQEVQDFLE
                     CIATGRQPLSDAQLAADVVAVTYAAYQSACEGRRIRL"
BASE COUNT          272 a          558 c          495 g          311 t
ORIGIN      
        1 cggcagggct tcgctcgcaa gcagcgcggt gatttatcgc cgcgcgccgg cttgcgcgct
       61 gcctccgttg cttcggattc cttatctcgg aatctaagcg ggcgggccgg ccgaaagcgc
      121 cagcgcccgg tcctggcgcc ctcggcccaa agtcgcaacc gtgatcgcgg cgtgctactc
      181 tacgggcacc cgcttgccgt gcgccgcttc ctcggccttc gggagagtga ccttcagcac
      241 accatcctca aacttcgcct ttacctcgtt ggccttgact ggggtgggca gagggatact
      301 ccgcatgaac cgcccatagc ggcgctcgct gcgataatag ccctcttcct tcttctccgc
      361 ctcgcgcttc atctcgccgc tgatggtcag agtgctgtcc tcaagcgtca cttccacctc
      421 ttcgcgcttc atgccgggaa gctccgcctc gacgaccaca ttcccatccc tgtcatagac
      481 atcgatgctc ggcacccaca tcgtcgcccc gaactccgca cgccgctccg ggagttcgaa
      541 gaagcgatcc atcatgcgct ccatctcatc gcgcaatcgg ctcatctctg tgaagggaga
      601 ccatcgtgtc agcgccattc tgctcctctc tcctttcagg catgacccag gcgaaacgct
      661 agcgggcggt ccgggcgtca caatgccgct cgctactggg cgtcggatgg tagtcacgtc
      721 tgcctgttcc agggacatgc cggacccaag agctgcgcgc atgccgtttc ccatggcata
      781 gggcgcttcc ggtctgatgt gaccataccc aggcaatctg gagtccaaac ggaggaggct
      841 cggcaccctc tggcggtatt ccctcgggct acaagcggat gcgtcgacct tcgcacgccg
      901 actgataggc ggcataggtg accgccacga catccgcggc aagttgtgca tcggagaggg
      961 gctgcctgcc cgtcgcgatg cactccagga agtcctgaac ctcctggggg tagccctgga
     1021 accagtcctc attgatgctg gggaagctcc accccgcctt ggtatcgatc ttctcggcga
     1081 tgtactcgtc cgcgaacacc tcgggcgcgg gcgcgtaggc gatgcacgtg gtgttgggat
     1141 tgaggttgca gtcaatccgg gcgttagaga ggtagagctc catccggttg cggatgccgc
     1201 ccaggacgat atccgaggcg ctgatggtcg cccgcgcgcc atcctcgaag ttgaggatga
     1261 gcgtggccca atcctccaca tccttccatc cagaggcaac ccacttccgc tcctcgcgct
     1321 cgaagctggg aatgcgggtg acgttggtcg tctcgcacga gacggaggcc acccggatcg
     1381 gcttgccatc acgccgctcg ccctccacgc gcttgaaata gagcgcggcc gcgagcggat
     1441 gggagccgag acgcaggagg gagccgcccc cggaagtgcg ccactcgcgg gcataggggg
     1501 agtgcgaccc gctgtgcgcc tcctcggcgc gaatgtcaaa gatcgtgccc ccggcggcct
     1561 cggccagacg gagcgccttc tgaatggccg gcgcgtagat ccagttctcc gcgtacatga
     1621 agagcacccc ggcgct
//
LOCUS       DAOSCV010001005         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50244_length_1633_cov_3.824461, whole genome shotgun sequence.
ACCESSION   DAOSCV010001005 DAOSCV010000000
VERSION     DAOSCV010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50244_length_1633_cov_3.824461"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1633
                     /locus_tag="PLY56_18510"
     CDS             <1..>1633
                     /locus_tag="PLY56_18510"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ23523.1"
                     /translation="DSAGNYRIDGLPAGLYQVAAVPSQESGWLPRSVSGVRVTIGEAA
                     EENLSLATAASLSGRVTDASGTPVVGARVTLRHGDGTSISGDTWVGGAYSIPGLRPGT
                     YRLTVSPPSELNLLDVAIADVRIRPEENATRDIALPSGGILQGVVRTADGSPVPYPQV
                     QLLTARDEARTVVGNAVGEYSFNGLHTGRYDLVALPTQAGANRPAPAFVPSVLVREGE
                     TASQELVLPPGGTVLVKVVDAAGNPVPEADVELSIQEPKAFEAAWGYRYTDRSGNVRF
                     TEVHDLADSYEPHSLPSCVATVTVESRSQGELLPAQITSVRVQAGQETVVTVTLDTGG
                     TITGRIEDATGAGVAGVFVRLPEVDLSDPEGPYSPELPLAAESDNNGVYHISRVPAGT
                     HTLVFDPRDANPLLLERRVTGVQVTPGASVTRDVVLDQGAMLSGAVTDAATGSSVPEA
                     VVALYRDADSSGTPETQAVTDSTGKYLLAGVAPGSYLVVVRGPDYRNLAPVSARLSLA
                     LGQEATHDVALPAGGVVAGVIRDETGEPVPGVLVEA"
BASE COUNT          259 a          520 c          577 g          277 t
ORIGIN      
        1 ccgacagcgc gggcaactac cggatcgacg ggctgccggc cggtctctat caggtggcag
       61 cggtgcccag ccaggagagc gggtggctgc cgcgcagcgt ttccggggtg cgcgtcacca
      121 tcggagaggc tgcggaggag aatctgagcc tggcaaccgc cgcgagcctg agcgggcgcg
      181 tgacggatgc gagcggcacg cctgtcgttg gcgcgcgggt cacgctccgg cacggcgatg
      241 gaaccagcat ctctggtgat acatgggtgg gcggcgccta ctcgatccct ggcctgcgcc
      301 cgggcacgta ccgcttgacc gtctcgccgc catccgagct gaacctcctg gatgtcgcga
      361 tcgcggatgt gcgcatccgg ccagaggaga acgccacccg cgacattgcc ctgccgagcg
      421 gcgggattct ccagggggtc gtgcgcaccg cggatggctc ccccgtcccc tatccacaag
      481 tgcagctgct caccgcgagg gatgaggcgc gcaccgtggt tggcaacgct gtcggtgagt
      541 actccttcaa tggactgcac accggccggt atgacctggt ggcgctccca acccaggccg
      601 gggcgaaccg gccagcgcct gcgttcgtgc catcggtctt ggtacgggag ggggagactg
      661 cttctcagga gctggtgctt ccacctggcg gcactgtgtt ggtcaaggtg gttgatgcgg
      721 ctggcaaccc ggtgccagaa gcggatgtcg agctctcgat ccaggaaccg aaggcattcg
      781 aggccgcctg gggataccgg tacactgatc gaagcgggaa tgtacgcttt actgaggtcc
      841 atgatctcgc ggatagctat gaaccccata gcctgccgtc gtgcgtcgcc acggttacgg
      901 tagagtcccg gtcgcaaggg gaactcctgc ccgcccagat cacgagtgtt cgggtgcagg
      961 caggccagga gacggtagtt accgtcaccc tggataccgg gggaacgatc acgggtcgga
     1021 tcgaggacgc gacgggagcc ggtgtggccg gcgtctttgt ccgtctgccg gaggtggatc
     1081 tgtcagaccc agaagggcca tactctccgg agctgccgct tgccgcggaa tcggataaca
     1141 acggcgtcta tcacatttcc cgggttcccg ccggaacgca caccctcgtg ttcgatccga
     1201 gggatgcgaa cccgctcctc ctcgagcgga gggtcaccgg cgtccaggtc acgccgggtg
     1261 cgtcggtcac gcgcgacgtg gtgctcgatc aaggcgccat gttgagcggc gcggtcaccg
     1321 acgcggctac cggatcgtcg gtgcccgaag cggtggttgc gctctatcgc gatgccgatt
     1381 catcgggaac tccggaaacg caggcggtga cggactccac cggcaagtac ctcctcgctg
     1441 gcgtggcgcc tggctcctac ctggtggttg tccgcggtcc ggattaccga aacctggcac
     1501 ccgtgagcgc gcgcctgtcg ctagcgctgg ggcaagaggc cacgcacgac gtcgcgctgc
     1561 ccgcgggcgg cgtggtcgcg ggcgtcatcc gtgatgaaac gggcgagccc gttcctggcg
     1621 tgctcgtgga ggc
//
LOCUS       DAOSCV010001006         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50349_length_1631_cov_2.316624, whole genome shotgun sequence.
ACCESSION   DAOSCV010001006 DAOSCV010000000
VERSION     DAOSCV010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50349_length_1631_cov_2.316624"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1382
                     /locus_tag="PLY56_18515"
     CDS             213..1382
                     /locus_tag="PLY56_18515"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOJ23524.1"
                     /translation="MNWKNASTAIGALAAGATTMFLLDPAQGRRRRAMVRDKMVRAGR
                     VTTDATGVIARDVANRAQGMLAATRSRLRREEDLSPEKIKARARAVLGRVVSHPRAIS
                     VDSTDGTLIVSGYILEREVEPLLRALQGVPGVRQVENRLEVGENPAEMPSLQGGRPRP
                     GMVPDILQRRMAPATRVALGGASIALAGYGAGRRDTAGSILAGLGALLGLRAALNMPM
                     RQIFGVWSTPAAIRIQNAIEIEAPVDEVFDFFRHFENFPRFMQHIRRVSQGEGGVWRW
                     EADGPLGIGVSWEAVITRVQQNRRISWKSLPGSEIANSGSVWFEETGRGTTRVHIQMC
                     YNPPAGAVGHALTSLLGANPVRQMDADLVRLKSLIERGKTTAHGERVTREEVRAS"
BASE COUNT          258 a          523 c          578 g          272 t
ORIGIN      
        1 agagccatga gtcgtgccat tggcgctacc attcggcaag tgcaccccgt ctccctcctg
       61 gcgcgaccgg tttcgggcgt ggaagcgccg atgtgatggc gtgcgcgcgg attgggtatg
      121 aggggcacgg cgcatccggc tgtatcgcgg gtcgggcgcc ctgccgtgag ccgtgggatc
      181 ggccggcaga tcctagccaa cggagaaagc atatgaattg gaagaatgca tcaacggcaa
      241 tcggcgctct cgcggccggc gccacaacga tgttcctgct cgatcccgct caaggccgcc
      301 gtcgccgcgc aatggtgcgc gacaagatgg tccgcgcagg ccgtgtgacc actgacgcga
      361 cgggcgtgat cgcgcgcgat gtggccaacc gcgcgcaggg catgctcgct gccacccgct
      421 ctcgtctccg gcgtgaagaa gacctatcgc ccgagaagat caaggcgcgg gcacgtgccg
      481 tgctgggtcg cgtcgtctca catcctcgcg ccatctcggt cgatagcacc gacggcacgc
      541 tgattgtctc gggatatatc ctggaacgcg aggtggagcc actcctgcga gcgttgcagg
      601 gggtgcctgg agtgcgccag gtggagaacc ggctggaggt gggcgagaac ccggcagaga
      661 tgccgagcct tcaagggggg agaccccggc cgggcatggt tcctgacatc ctccagcgac
      721 gcatggcgcc ggcgacgcgg gtcgcgcttg gtggcgcgtc tattgccctg gccgggtatg
      781 gcgcggggcg gcgcgatacg gccgggagca tcctggcggg cctgggcgcg ctgctcgggc
      841 tacgggcggc gttgaacatg ccgatgcgac agatcttcgg agtgtggtct acaccggcag
      901 cgattcggat tcagaacgcc atcgagatcg aggcgccggt ggatgaggtg tttgatttct
      961 tccgccactt cgagaacttc ccccgcttca tgcagcacat ccgccgcgtg agccagggag
     1021 agggcggcgt gtggcgctgg gaggccgacg gcccgctcgg catcggtgtc tcctgggagg
     1081 ccgtcatcac gcgggtgcag cagaaccggc gcatctcctg gaagagtctg cccggctcgg
     1141 agatcgccaa ctccgggagc gtttggttcg aggagacggg ccggggtacc acccgggtgc
     1201 atatccagat gtgctacaac ccgcctgcag gcgccgtggg tcatgcgttg acctccctcc
     1261 tgggagccaa cccggtacgc cagatggatg cggacctggt gcgcctcaag tcgctgatcg
     1321 agcgcgggaa gacgaccgca cacggcgagc gggtgacgcg cgaggaggtc cgggcgtcgt
     1381 agagccccct cgccgtgcat tggaagacgg ccggcgggtc cacctccccg gaatccgcag
     1441 gggtgggccc gccggcacgc atctcccgcg tatccttcca cgagcacccc gcgcctccgg
     1501 ggccccggcc agcggatcca atccttgacg ctcttttcat ctccgtgata caataacgcc
     1561 tgggatacct ggctcatgca cctgctggcg gggcggtctc ggggcgccgc agtgcctagc
     1621 ctgtctgtct c
//
LOCUS       DAOSCV010001007         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50438_length_1629_cov_2.459339, whole genome shotgun sequence.
ACCESSION   DAOSCV010001007 DAOSCV010000000
VERSION     DAOSCV010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50438_length_1629_cov_2.459339"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLY56_18520"
     CDS             complement(<1..848)
                     /locus_tag="PLY56_18520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23525.1"
                     /translation="MQRLRIAILGAGTGRGQSWQSTLRKLSERSDLYDFCAVCEVIEE
                     RARQNAERWGVKPYTSLTAMLAEEKLDVLLNGVSSDSNPMAVGLAAKHGVHPITEIPI
                     APTLRIADYMVQTCERHRVKLEVTEQVFLWAREQLKRKIIESGIIGEVTHARLWYTNK
                     ADYHGLNAVRMLLPGEATRVLVYSGKVRVPHFVGYEGELVQEDSWDAAIIEFDSGVVC
                     LFEDPPRGRMSSRWDIEGTLGQLVGNDLYIGSVERATHYPFLYEYTTIDGEKVLDHLR
                     VETDPP"
     gene            complement(996..>1629)
                     /locus_tag="PLY56_18525"
     CDS             complement(996..>1629)
                     /locus_tag="PLY56_18525"
                     /inference="COORDINATES: protein motif:HMM:NF014845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23526.1"
                     /translation="PALKLLSGEFIRCQIGPEEVAAFHERALKELLPTLPEERVAPAS
                     LQAAAFLRLALRGFGVDPLRPYLETSVTSRFVGQILRELEAEFHLPDVARIRIGSKYA
                     ENFEGDLANYLDMFRLQGLGNLTLKSVDEAHGELLFTGRDVFESYEASDYPQDHFTRG
                     FLARAVSVLTRRQMNCEEVACQAQGAPECRFVVTPVAPGPIPNLRRLLDG"
BASE COUNT          283 a          530 c          529 g          287 t
ORIGIN      
        1 atgggcgggt cggtctccac gcgcaggtga tccaggacct tctcgccgtc gatggtggtg
       61 tattcgtaca ggaagggata gtgcgtggcg cgctcgacgg agccgatgta gaggtcattc
      121 ccgaccaact ggcccagggt gccctcgatg tcccagcgcg agctcatccg gccccggggc
      181 gggtcctcga agaggcagac cacgccgctg tcgaactcga tgatggcggc gtcccacgag
      241 tcctcctgga ccagctcgcc ttcatagccg acgaagtgag gcacccgtac cttgccggag
      301 tagacaagca cgcgggtggc ctcgcccggc aggagcatgc gcaccgcgtt gaggccgtga
      361 tagtcggcct tgttggtgta ccagaggcgt gcgtgcgtaa cctcgccgat gatgccgctc
      421 tcgatgatct tccgcttgag ctgctcgcgt gcccagagaa agacctgttc cgtcacttcc
      481 agcttcacgc ggtggcgctc gcacgtctgc accatgtaat cggcgatgcg caaggtaggc
      541 gcgattggga tctcggtgat cgggtggacg ccgtgctttg cggccagccc cacggccatg
      601 gggttgctgt cggaggagac gccgttgagg agcacatcga gcttctcttc ggcaagcatg
      661 gccgtcaagc tcgtgtaggg tttcacaccc caccgctcgg cgttttgccg tgcgcgctcc
      721 tcgatcacct cgcagaccgc gcagaagtca tacagatcgc ttctctcgga gagcttgcgc
      781 agggtggact gccaggactg gccgcggcca gtgccggctc ccaggatggc gatgcgaagt
      841 cgttgcatgt gagttccccc aaaggtgtct ccaggacgcg ggctgtccgc ccgcggtatc
      901 cccgcgttgg aagcatccgc tcgatgccgt tttcccgtgc caccgcccag acgagcgcgg
      961 cccaacgcca tcctaaccta ctccgagtgc cgcggtcacc cgtccagcaa gcggcggagg
     1021 tttggaatag ggccgggagc gaccggtgtc accacgaagc ggcactcggg agcgccttgc
     1081 gcctggcagg cgacctcctc gcagttcatc tgtcggcggg tcagcaccga cacagcgcgc
     1141 gccaggaagc cccgggtaaa atggtcctgc gggtagtccg aggcctcata gctctcgaac
     1201 acgtctcgtc cagtaaagag caactcaccg tgggcctcat ccaccgattt caaggtcagg
     1261 ttgcccaagc cctgcagccg gaacatatcg aggtagttcg ccagatcgcc ctcgaagttc
     1321 tccgcgtact tactcccgat gcggatgcgc gcgacgtctg gtaggtggaa ctctgcctcc
     1381 agctcgcgca gaatctggcc cacaaagcgg ctggtaaccg acgtctccag gtaggggcgc
     1441 agcgggtcga caccgaagcc gcggagtgcg agccgcagaa aggcggccgc ctggagggaa
     1501 gccggggcaa cccgctcctc ggggagggta ggcagcaact ctttcagcgc gcgctcgtgg
     1561 aatgccgcca cctcctccgg accgatttgg cagcggatga actcgccgct cagaagcttc
     1621 agcgccggc
//
LOCUS       DAOSCV010001008         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50663_length_1624_cov_3.094328, whole genome shotgun sequence.
ACCESSION   DAOSCV010001008 DAOSCV010000000
VERSION     DAOSCV010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50663_length_1624_cov_3.094328"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..1116)
                     /locus_tag="PLY56_18530"
     CDS             complement(160..1116)
                     /locus_tag="PLY56_18530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOJ23527.1"
                     /translation="MALVSIIIPAHNAEEYLKGAIHSLLRQTYPWVEIIIVDDGSTDG
                     TAAVVRGFGNQVRSLYQVNAGPSAARNAGLARARGKYVAFLDADDQFHPERIEILVRA
                     LEAASPNATFAVSDAWLWDGTRCLRRYNVSEGPAGERGLTRFLDDNRPYIGILARREA
                     LVGVGGFRQDLWLNEDLHLWLRLLADGNTYVHVPEPLYLYRVRHHSLSRKWQLQLEGA
                     CQLYLEALGRSSLSSGQRRRLAYLLWRDRARLHAAEAHRARAEGQPMRRIAFLGRYLG
                     AQSVRMLLRPHFTARRLWSKRNGEPCVLEEIPGPRDKHLLTR"
     gene            1296..1574
                     /locus_tag="PLY56_18535"
     CDS             1296..1574
                     /locus_tag="PLY56_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOJ23528.1"
                     /translation="MPKARLHAMVHGRVQGVGFRYWVVEQAQRLDLTGWVRNRPGREV
                     EVLAEGERVDLEQLLERLKDGPLMARVTDVDADWSEYRGDFDRFGVTW"
BASE COUNT          290 a          488 c          570 g          276 t
ORIGIN      
        1 gctttctggt gggccggcca tctgaagccg ttcgtgtgga accacgagcg cctgggtact
       61 ctcctgcgcg agggacgcag gcgggccacg cttctcctaa agcggcgctc ctaagacagg
      121 ctggacgggg cagaggcccg agtgcgcgct ggcatcggtt cagcgcgtca ggagatgttt
      181 gtctcgaggg ccggggatct cctcgagcac gcacggttcg ccgttccgct tgctccagag
      241 gcggcgggcc gtaaagtgcg ggcgcagcag catgcgcaca gattgcgcgc caagatacct
      301 tcccaggaag gcgatccgcc gcatgggctg gccttctgcc cgagcgcgat gcgcctctgc
      361 cgcatggagc cgggcacggt cgcgccagag gagataggcc aggcggcggc gttgccccga
      421 ggagagcgag cttctgccga gcgcctcaag atagagctgg caggcgccct cgagctggag
      481 ctgccacttg cgcgagaggc tgtggtggcg cacccggtag aggtagagcg gctccggcac
      541 gtggacatag gtgtttccat cggccaggag gcgcagccac aggtgcagat cttcgttgag
      601 ccacagatcc tgccggaacc ccccgacgcc gaccagggct tcccggcgcg ccaaaatgcc
      661 gatatagggg cgattatcat ccaggaagcg ggtgagccca cgctcgcccg ccgggccttc
      721 ggagacgttg tagcggcgca ggcagcgtgt gccatcccag agccacgcgt ctgagactgc
      781 gaaagtcgcg ttcggcgagg ccgcttccag cgcgcgcacc aggatttcga tccgttccgg
      841 atggaactga tcgtcggcat cgaggaatgc cacgtacttg ccccgggcac gcgccagccc
      901 cgcattgcgc gcggcgctcg ggccggcgtt cacctggtaa agagagcgta cctgattgcc
      961 aaacccgcgc acgaccgccg cggtgccatc ggtggagcca tcatccacga tgatgatctc
     1021 tacccacgga taggtctgcc ggagtagcga gtggatcgcg cccttcaggt actcctcggc
     1081 gttatgtgcc ggaatgatga tgctgaccag ggccatcgac acactcctcc cataagccat
     1141 gagggcgcaa gaagcgcgcc atacctatcc ccgagcattc atgcggagca gatggaacgc
     1201 gagccacgcg ggattcgctc gccaggcgct tgaccagggc cggtcttcgt ggtacattga
     1261 acgctggcca ccaagtttgg gaggaggaga cgaggatgcc gaaggctcgc ctgcatgcga
     1321 tggtgcatgg ccgcgtgcag ggtgtggggt ttcgctattg ggttgtggag caggcacagc
     1381 ggcttgatct gactggatgg gtacgcaacc ggccggggcg cgaggtggag gtgctcgctg
     1441 aaggagaacg cgttgatctg gaacaacttc tcgagcgcct gaaagacggc ccgttgatgg
     1501 cgcgcgttac ggatgtggac gcggattgga gcgaataccg gggggatttt gaccggtttg
     1561 gcgtaacctg gtagaccgcg ccgggccacg gcggaatcgc agtagcccgg cgcgttattc
     1621 tttg
//
LOCUS       DAOSCV010001009         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50665_length_1624_cov_3.015934, whole genome shotgun sequence.
ACCESSION   DAOSCV010001009 DAOSCV010000000
VERSION     DAOSCV010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50665_length_1624_cov_3.015934"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..879)
                     /locus_tag="PLY56_18540"
     CDS             complement(46..879)
                     /locus_tag="PLY56_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ23529.1"
                     /translation="MQWIMFSKMLGTLSVAEAGERIREIGFEGVDLTVRPGGHVLPEN
                     ALRDLPPAVETLRSLGLSVPMITTSVTAADEEHTRNIFQAAAECGIHRLKLGYWRYEG
                     FGSMAAQIEEIKRKLEGLARLAREYGVTACIHCHSGPFMSADPAVVWVLLRDFDPDHL
                     GAYIDPGHMTVEGGVAGWKMGLDLLAPHIRLMAVKSFAWFREESDGRVGWIHRLVPLR
                     EGPVRWSEVFDCLRQIGYDGPVSLHSEYQGRHSWRDLSLDELLEQTRDDLAYIRQVAG
                     C"
     gene            1081..1275
                     /locus_tag="PLY56_18545"
     CDS             1081..1275
                     /locus_tag="PLY56_18545"
                     /inference="COORDINATES: protein motif:HMM:NF025156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HOJ23530.1"
                     /translation="MQRWEYKIAYRSESSGEWFIDGRKAGDLGKAEEPEVLNRLGQEG
                     WELVSVVGYTYFFKRLVKER"
     gene            complement(1286..>1624)
                     /locus_tag="PLY56_18550"
     CDS             complement(1286..>1624)
                     /locus_tag="PLY56_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23531.1"
                     /translation="TGETGQELTYADTENKLLFYAGADPSINPETYFTSPSFNPDDHY
                     IHLNDNNCTYRGSMYAPYGRILVESNKVAIYGSIIGDTIGITQYANNLTVSFDPLLSP
                     PVSPGMLLIE"
BASE COUNT          316 a          500 c          514 g          294 t
ORIGIN      
        1 acaggctccc taagcagagc gcactccctc gtctcgggcg tgggatcaac agccggcgac
       61 ctggcggatg taggcgagat cgtctcgcgt ctgctccagc agttcatcta gtgagaggtc
      121 gcgccacgaa tgtctcccct ggtactcgct atggagcgag acggggccgt cgtagccaat
      181 ctggcgcagg caatcgaaca cctcgctcca gcgcaccggg ccctcccgaa ggggcaccag
      241 gcggtggatc cagccaaccc gcccgtcgct ctcttcccgg aaccaggcga agctcttcac
      301 cgccatgagc cggatgtgag gagccaggag gtcgagcccc atcttccatc cagcaacccc
      361 gccctcgacg gtcatatgcc cgggatcgat gtaggcgccc aggtggtcgg gatcgaagtc
      421 acgcaggagc acccagacga ccgcggggtc ggcgctcatg aaggggccgg agtggcaatg
      481 gatgcacgcg gtgacgccat actcccgcgc cagacgggcg aggccctcca gttttcgctt
      541 tatctcctcg atctgtgccg ccatcgagcc aaagccctcg tagcgccagt agcccagctt
      601 gagccggtgg ataccacatt cggcggcggc ctggaaaatg tttcgggtgt gctcttcatc
      661 cgccgcggtc acgctggtgg tgatcatcgg cacgcttaag cccaaggagc gcagtgtctc
      721 tacagccgga gggagatcgc gcagagcgtt ttccggaagc acatggcccc cggggcgcac
      781 cgtcagatcc acgccctcga acccgatctc tcggatccgc tcgcccgcct cagccacgct
      841 cagagtgccg agcatcttgg aaaacatgat ccactgcatc gtctttcctc tcatccacga
      901 gcctacatgt cagattcccc accccaagct gcgcctcctg cgcgtgcatc ttgcgcgcag
      961 gcccaccgcg ggtgcagaaa ccgcgagaag tgggaagaat ccctcgcgga aggagcgccc
     1021 gatgcgggac gcgccgggag ccaccgtcat ccccgtctgg cggatggaga ggagccgcga
     1081 atgcaacgct gggagtataa gatcgcctat cggagcgaat cgtcgggaga gtggttcatc
     1141 gatggccgga aggcaggcga cctggggaag gcggaggagc ccgaagtcct gaaccgcctc
     1201 ggccaggaag gctgggaact cgtgagtgtc gtcggttaca cctacttttt caagcgtctg
     1261 gtgaaggaga ggtagaacac cacactcact cgatcagcaa catgcccggc gagaccggcg
     1321 gcgagagcaa cgggtcgaag ctcaccgtga ggttgttggc atactgggtg atcccgatcg
     1381 tatcgccaat gatcgatccg tagatcgcga ccttgttgga ctcgactaga atccggccat
     1441 agggtgcgta catggagccc cggtaggtgc agttgttatc attcaagtgg atgtagtgat
     1501 cgtctgggtt gaagctcggt gacgtgaagt aggtctccgg gttgatgctg ggatcggcgc
     1561 cggcatagaa gaggagtttg ttctctgtat cggcataggt aagctcctgg cccgtttcgc
     1621 ccgt
//
LOCUS       DAOSCV010001010         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50702_length_1623_cov_4.352679, whole genome shotgun sequence.
ACCESSION   DAOSCV010001010 DAOSCV010000000
VERSION     DAOSCV010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50702_length_1623_cov_4.352679"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLY56_18555"
     CDS             <1..755
                     /locus_tag="PLY56_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HOJ23532.1"
                     /translation="TLCPWNSPDTPAEQVAETYHAALRGVPSGALDWYLSIKAPEIDY
                     SLQLTQTVVAAAGQLGIGVHFDSHGPETADPTFDLVERLLPGASPLGCTLPGRWERSL
                     RDAERVREWGVRVRVVKGQWREPGRRERDPRLGCLEVIECLAGNAQRVAVASHDPVLV
                     RDGLTRLLKAGTPCELELLFGLPMVPVLNVARELGVGVRVYVPYGHAWLPYCFTQMAR
                     NPRILWWLIRDMTFRRSARALAAGMGAASGPA"
     gene            786..>1623
                     /locus_tag="PLY56_18560"
     CDS             786..>1623
                     /locus_tag="PLY56_18560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ23533.1"
                     /translation="MAKKPVDGVVPGGPIFECVTTAERLGELESAWDALHARSPGAHL
                     FQSYPWVSSWIRHRPEGRPLVLTARRDDRLTAALALCAHDVGPVAFGIRTLRVATDYS
                     DFCDALSDPDYPEDLPAAWANFLGHAPWNVIDLHYVRPGSALASLCDQVPHAWRIHRA
                     QQDVAPYLDLSIDWRETVGKGHRSDWLRRRRRLEERGGLALTRAQTPAEIDALLEEFA
                     RFHIERWRAKGETSAYSFQETREFLRDVCHGLQRRGQLRLYRLAAGGDTVSIGIYTLF
                     GSR"
BASE COUNT          234 a          520 c          585 g          284 t
ORIGIN      
        1 tgaccctctg tccctggaac tcgcccgata ccccggctga gcaagtcgcc gagacgtatc
       61 acgcggcgct caggggcgtt ccatctggcg cgctcgattg gtacctctcc atcaaggcgc
      121 ctgaaatcga ctactcgctc cagctcaccc agacggtggt cgccgcagcc gggcagcttg
      181 ggatcggggt ccacttcgat tcccacggcc cggagaccgc cgatcccacg tttgatctgg
      241 tggagcggtt gctgccgggc gcttctccac tggggtgcac cctgccgggg cgatgggagc
      301 ggagcctgcg agacgcggag cgcgttcgcg agtggggcgt gcgcgtgcgg gtggtaaagg
      361 gccagtggcg agagccaggc cggcgcgagc gcgacccacg cctgggttgc ctggaggtca
      421 tcgagtgcct tgccgggaat gcccagcgcg tggcggtagc ctcccatgat cccgtgctgg
      481 tgcgcgatgg gttgacccgg ctgctcaagg cgggaacgcc atgcgagctg gagctcctct
      541 tcgggctccc gatggtgcca gtgttgaacg tggcgcgcga gctgggggtg ggcgtccggg
      601 tatacgtgcc ctacgggcat gcgtggcttc cctactgctt cacacagatg gcacgaaacc
      661 cgcgcatcct ctggtggctg atccgcgaca tgacgttccg gcggtcggct cgggccctcg
      721 cggccgggat gggcgcggca tccggcccgg cgtagcgcgc tgcgcggcgg tgaacggagg
      781 gaaacgtggc gaagaagccg gttgatgggg tagtgccggg agggcctatc ttcgagtgcg
      841 tcaccaccgc cgagcgcctg ggcgagctgg agagcgcgtg ggacgcgctc catgcgcgca
      901 gtccgggggc ccacctgttt cagagctacc cgtgggtctc gtcctggatc cggcatcgtc
      961 cggaaggccg gcctctggtc ctcaccgcaa ggcgggacga ccgcctcacc gcggcgctgg
     1021 cgctctgcgc acacgatgtc ggcccggtgg cgttcgggat ccgcacgctg cgtgtggcca
     1081 cggattactc ggatttttgc gacgcgcttt ctgatcccga ctacccggag gatctgccgg
     1141 ccgcctgggc caacttcctc gggcacgcgc cctggaatgt gatcgacctc cactacgtgc
     1201 gcccgggatc ggccctggct tcgctgtgcg atcaggtccc ccacgcatgg cggatccatc
     1261 gcgcgcagca agacgtggcg ccttatctgg acctatcgat cgactggcgc gagacagtgg
     1321 ggaaggggca ccggtcggat tggctacggc gccgccggcg tctcgaggag cggggcggtt
     1381 tggcactgac gagagctcag accccggcgg agattgacgc gctcctggag gagttcgcac
     1441 gtttccatat tgagcgatgg cgggcaaagg gcgagaccag cgcctactcc ttccaggaga
     1501 cgcgcgagtt cctgcgtgat gtctgccacg gcctgcaacg ccgaggccag ctgcggctgt
     1561 accgattggc ggcaggtggc gataccgtca gcattgggat ctacacgctg tttgggtcgc
     1621 gct
//
LOCUS       DAOSCV010001011         1622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50743_length_1622_cov_4.770262, whole genome shotgun sequence.
ACCESSION   DAOSCV010001011 DAOSCV010000000
VERSION     DAOSCV010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50743_length_1622_cov_4.770262"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLY56_18565"
     CDS             complement(<1..378)
                     /locus_tag="PLY56_18565"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23534.1"
                     /translation="MGLKIGICGAGKFGRCFVPVFQRHPDVEEVYLADVIPDRLAQVA
                     AQYGVQKTFDSLDALCKSDCDAIALFTQRWMHGPQAVQALKAGKHVYSAVPAAITLEE
                     LAELVDTVKSTGLIYMLGETSYYR"
     gene            complement(557..1510)
                     /locus_tag="PLY56_18570"
     CDS             complement(557..1510)
                     /locus_tag="PLY56_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 domain-containing protein"
                     /protein_id="HOJ23535.1"
                     /translation="MRITGTFLDEISHDIPHQNWGPEEWDLDFQAMRAIGIDTVILIR
                     CGYRRWMTYPSQVLQEREGCYPPPLDLVDLFLTLAEKYGMEFWFGTYDSGRYWHQGQF
                     QEEVDINLRVLDEVWARYGHRPAFKGWYLSQEVSRRTGGIIDIYARMGRHCKEISGNL
                     PTLISPWIQGVKAVSAFTGQTRQAQGITLEEHEREWNEIMAGIAGAVDIVAFQDGHVE
                     FDELASFLSLNKALAERHGLRCWTNSESFDRDMPIKFPPIKWEKLLLKLNAAEQAGME
                     KAITFEFSHFMSPNSIYRAAHHLYRRYCEQFGLEPWQPPAQ"
BASE COUNT          290 a          525 c          490 g          317 t
ORIGIN      
        1 ccggtagtag ctcgtctcgc ccagcatgta gatgagcccg gtcgatttca cggtgtcgac
       61 cagctcggcc agctcctcca gggtgatcgc ggcgggcacg gcggaataga cgtgcttccc
      121 ggccttcaac gcctggaccg cctgtggccc gtgcatccac cgctgggtga agagggcgat
      181 cgcgtcgcaa tcactcttgc aaagcgcgtc gagcgaatcg aacgtcttct gcaccccgta
      241 ctgcgccgcc acctgcgcca gccggtccgg gatcacgtcg gcgagataga cctcttccac
      301 atccgggtgg cgctggaaca ccggcacgaa acatcttccg aacttcccgg ccccgcagat
      361 cccgatcttg agacccatct tcacgctcct taccccgcat tattcatcag aaataatgaa
      421 aagaagtctc tctgccaggc gtggcgcgga tttgtgggca ctgccctcga aaacactctg
      481 cttccgggcg cttgtggcgg caacggcgtg ccaccagaca agagacttct cgaagtattc
      541 tggtgaatac ttcgggttac tgcgctggcg gctgccacgg ctccaggccg aactgctcgc
      601 agtagcgccg gtagagatgg tgcgccgcgc gatagatgga gttggggctc ataaagtgcg
      661 agaactcaaa ggtgatcgcc ttctccatcc cggcctgttc cgccgcgttc agcttcagca
      721 gcagcttctc ccacttaatc ggtgggaact tgattggcat gtcgcggtcg aacgactcgc
      781 tgttcgtcca gcagcggagg ccgtggcgct cggccaatgc tttgttcagc gagaggaagc
      841 tggcgagctc gtcgaactcc acgtgcccgt cctggaacgc cacgatatcc acggcgccgg
      901 cgattccagc catgatctcg ttccactcgc gctcgtgctc ttccagggtg atcccctgcg
      961 cttgccgcgt ctgcccggtg aaggcgctca cggccttcac gccctggatc cacggcgaga
     1021 tgagcgtggg caggtttccc gagatctcct tgcagtgccg gcccatccgg gcgtagatgt
     1081 cgatgatccc gccggtgcgc cggctcacct cctggctgag ataccacccc ttgaacgccg
     1141 ggcggtggcc gtagcgtgcc cacacctcgt caagcacgcg caggttgata tcgacctcct
     1201 cctggaactg gccctgatgc cagtagcgcc cggaatcgta ggtgccaaac cagaactcca
     1261 tgccgtactt ctcggccaga gtgaggaaga gatccaccag atcgagtggc ggcgggtagc
     1321 agccctcgcg ctcctggagc acctgtgagg ggtaggtcat ccaccggcgg tagccgcagc
     1381 ggatcaggat cacggtgtcg atcccgatgg ctcgcatcgc ctggaagtcg aggtcccact
     1441 cctcggggcc ccagttttgg tggggaatat cgtggctgat ctcgtcgaga aaagttcctg
     1501 tgattcgcat cgcgtgtacc tctccgcggt gttcgcctcg cgcttcgcgg ttcctgctcg
     1561 aacacgcacc caaggggaaa ccgcggcctg gagcgagaac cgatcaaagg caaggggtga
     1621 tg
//
LOCUS       DAOSCV010001012         1621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50805_length_1621_cov_2.736271, whole genome shotgun sequence.
ACCESSION   DAOSCV010001012 DAOSCV010000000
VERSION     DAOSCV010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50805_length_1621_cov_2.736271"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PLY56_18575"
     CDS             complement(<1..825)
                     /locus_tag="PLY56_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ23536.1"
                     /translation="MKSGGWLDIPWLKHGWWHYVPHCLLAVAVLLLFHRLRLVLAPFI
                     IAYLLAAALDPLVVQMEGRGWRRGRAVLCIYAVFLLLFLISLFTLLPVAIAQVGEFAR
                     EVPVYIEKAPRLFEDVRQRLGAIEIPQYLRDAITRHVGSIATTVAKRVAAWAQGIISS
                     FGFLLWLVLIPVATFFLLNDIQRVRASLLRLVPERQRSMVQAMAADVADVFIRYLRGL
                     AVVAVLYGLFILVTLLFLGVPYAVALGVMAGALYPVPYLGPFVTTATILVVGAVTEG"
     gene            1202..>1621
                     /locus_tag="PLY56_18580"
     CDS             1202..>1621
                     /locus_tag="PLY56_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23537.1"
                     /translation="MRAQPFRNPRLWASITMWSLLLVGVVALAPLAADSAQEGRGPFI
                     RLQYATFDPLERQPQVEQSLRMPPMAAGDTSRFLLQFQGPVRVQWLNAVRSLGVVLES
                     YVPDYTYIARMTLAQAAALPTRFSFVRWVGPFHPAYKI"
BASE COUNT          314 a          548 c          509 g          250 t
ORIGIN      
        1 cccttccgtc accgcgccga ccacgaggat ggtggcggtc gtcacaaagg gtcccagata
       61 cggaacgggg taaagcgcgc ccgccatgac cccgagggcg acggcatagg ggacgccgag
      121 aaacaacagg gtcaccagaa tgaacagccc gtagagcacc gcgacaaccg ccaggccgcg
      181 gaggtagcga atgaagacgt cggccacgtc ggcggccatc gcctgcacca tgctcctctg
      241 ccgctctggc accagccgga ggagactcgc gcgcacccgc tggatgtcat tgagcaggaa
      301 gaaggtcgcc accgggatga gcacaagcca gaggaggaag ccaaaggagc tgatgatccc
      361 ctgggcccag gcggccacgc gcttggccac cgtggtggcg atgctcccaa catgccgcgt
      421 gatggcatcc cgcagatact gagggatctc gatagccccc aggcgctggc gaacatcctc
      481 gaagagccgg ggcgccttct cgatgtagac cggcacctct ctggcgaact cgcctacctg
      541 ggcgattgcc accggaagga gggtgaagag cgagatgagg aagaggagca agaaaacggc
      601 atagatacag aggaccgccc tgccccggcg ccagccacgg ccttccatct gcaccacgag
      661 ggggtcgagc gccgctgcca ggagataggc gatgatgaac ggcgcgagca ccaggcgcag
      721 ccggtggaac agcaggagca ctgcaaccgc cagcaggcaa tgcggaacgt agtgccacca
      781 gccatgcttc aaccagggaa tatcgagcca cccaccactt ttcatccgct gctccaagcc
      841 cccccaggaa aatgcatgcg cctgacaagg atggaatccg aaccgaaggc tctcccgagc
      901 gatgccgggg tgcgcatgca tggttgccgg ctttttctgg aacgccgtcc cggaatcctt
      961 caccttgact ccctttcacc cttgtgttat actgcttccc ggtggtcttg cgggtctcaa
     1021 cggcccgcgt cacctgctcg tcccagtggc atggccgcgg ccccaccggg cttgccggca
     1081 tcgtcgcgcc ccgcccgcca cacccaggca gggggagagt gccgcgcagg ctctggaagg
     1141 tatcaggccc gaggcctgta accgcccggt ccatgagcgc gacccggggg aggagactgc
     1201 cttgagagca caaccttttc ggaatcctcg tctttgggcc tcaatcacca tgtggtccct
     1261 gctgcttgtc ggagtggtcg cgctagcgcc cctggcggcg gatagcgccc aggaaggacg
     1321 cggtcccttc atccgacttc agtacgcaac gttcgatccg ttggagcgcc aaccccaggt
     1381 ggagcaaagc ctgcgcatgc ctcccatggc ggccggcgac acctcgcgct tcctgctcca
     1441 gttccagggt ccggtgcgcg tgcaatggct gaacgccgtg cgttcgctcg gcgtcgtcct
     1501 cgagagctac gtgccggact acacctacat tgcccgaatg acgctggcgc aggcggccgc
     1561 gctgccgacg cggttctcct tcgtccgctg ggtgggcccc ttccatcccg cctacaagat
     1621 a
//
LOCUS       DAOSCV010001013         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50836_length_1620_cov_4.280511, whole genome shotgun sequence.
ACCESSION   DAOSCV010001013 DAOSCV010000000
VERSION     DAOSCV010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50836_length_1620_cov_4.280511"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PLY56_18585"
     CDS             <1..661
                     /locus_tag="PLY56_18585"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOJ23538.1"
                     /translation="LNPYAVSELIAGLAWAGLGALSVQERRSFMNDRFGEQITGEKIT
                     LFDDGLDRAGLPVPFDAEGVAKQRVDLIVKGCANAVVYDSYTAHREGKQSTGHATGGS
                     GVWGPYPQNLFLEPGDSDLDTMIRATERGVLITRFHYTNLVHPTKTVLTGMTRDGTFW
                     IENGQIAYPVKNLRFTQSILDALASATAIGRERKLVDVGWACVPALKLERFEFSGTTE
                     F"
     gene            663..1484
                     /locus_tag="PLY56_18590"
     CDS             663..1484
                     /locus_tag="PLY56_18590"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOJ23539.1"
                     /translation="MDLVFPAWASDTHQSLLEALPAMAAQGVTRVEWGLGNPDYFDAK
                     DAAQVAAVRQVLADTGIRAVALHAPFGEQADLASLESRVREVTIACYVDAINVARQVG
                     ARYLVVHAGHGPCNDRLAQRLLLARESLCALEPVARMAGVVLALENLPPGYPGSAAGE
                     LLDTLDAIGSPCVGICFDTGHAHLAGHLPATAGALLPRAVMIHLHDNDGAEDQHLLPG
                     CGAIDWGEFARIYQETGCTAPATLECSPPDGWGWDRCSAAVRELLGIEEIANGHS"
BASE COUNT          259 a          497 c          561 g          303 t
ORIGIN      
        1 tttgaatcca tatgccgtgt ccgagctgat cgccggcttg gcatgggccg gcctcggcgc
       61 gctcagtgtt caagagcggc gtagcttcat gaatgaccgc ttcggcgagc agatcacggg
      121 agagaagatc accctctttg atgacggctt ggaccgggcc ggcctccccg ttccctttga
      181 tgcggagggc gtcgccaagc agcgggtgga tctgatcgtg aagggatgcg ccaacgccgt
      241 cgtctacgat tcctatacgg cccaccgcga agggaagcag tccaccgggc acgccacggg
      301 cgggtcgggc gtctgggggc cgtaccccca gaacctcttt ctcgaaccgg gcgattccga
      361 tctcgatacg atgatccgtg ctaccgagcg cggggtgctc atcacccggt tccactacac
      421 gaacctggtg catccgacca agaccgtgtt gacggggatg acgcgcgatg gcaccttctg
      481 gatcgagaac gggcagattg cctatccggt gaagaacctc cgcttcaccc agtcgatcct
      541 cgatgctctg gcgagcgcca ccgccatcgg gcgcgagcgc aagttggtgg acgtgggctg
      601 ggcctgcgtg cccgcgctca aactggagcg cttcgagttt tctggaacga cggagttctg
      661 agatggatct cgtctttccg gcttgggcgt cagataccca tcagagtctc ctggaggcgt
      721 tgcccgcgat ggcggcgcag ggcgtgaccc gcgtcgagtg gggcctgggg aaccccgact
      781 acttcgatgc gaaggatgcc gcccaggtgg cggctgtccg ccaggtgctg gccgataccg
      841 gtatccgtgc cgtggccttg cacgccccat ttggcgaaca agccgatctg gccagcctgg
      901 agtcgcgggt tcgcgaggtg accatcgcct gctacgtgga tgccatcaac gtggcgaggc
      961 aagtgggagc gcgctatctc gtggtccacg cgggacatgg gccgtgcaac gaccgcctgg
     1021 cgcaacggct gctcctggcg cgggaaagcc tgtgtgcttt ggagccagtg gcccgaatgg
     1081 cgggcgtcgt gctcgcgctg gagaatctgc cgcccggtta cccgggaagc gcggcaggag
     1141 agctcctcga tacgctcgat gccatcgggt cgccctgcgt ggggatctgc tttgatacgg
     1201 gtcatgcgca tctggcgggt cacctgcccg cgacggcggg ggcgctcctg ccacgcgcgg
     1261 tgatgatcca tctccatgac aatgatggtg ccgaagacca gcatctgctc cccggctgcg
     1321 gtgccatcga ttggggggag ttcgcgcgca tctaccagga gacgggatgc accgcgccgg
     1381 ccacgctgga atgctcgcct ccggatgggt ggggctggga ccggtgttcg gcggcggtgc
     1441 gcgagttgct cggaatagag gagatagcca atggtcattc gtagtccacg atcttccgat
     1501 gaggttgccg cgcaggcgga tgtggcggca gctgctttcg ggcccgatgc gctgagcgag
     1561 ccatggcgag atcgctaccg cgccatcgag cagctcttcg ggcgcgatgc cttcctcgtc
//
LOCUS       DAOSCV010001014         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_50849_length_1620_cov_3.095208, whole genome shotgun sequence.
ACCESSION   DAOSCV010001014 DAOSCV010000000
VERSION     DAOSCV010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50849_length_1620_cov_3.095208"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PLY56_18595"
     CDS             complement(<1..1002)
                     /locus_tag="PLY56_18595"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ23540.1"
                     /translation="MTSRERIVAAMERRQPDRVPLMCQLSIGHYLLQCGVSGANYWLH
                     NETIIDCFVALAERYGFDGILINLPGRPPNADDFVDRIEQTPEGEVIHWKRSGRCLCP
                     PDDLPHEVETPRLALEEIDPERVFYEDPHTLGGLKYPFHYDLEPLSGSGDDFFPPYLT
                     AIIDGVIARVGKRLSVHSEVFSPFTQLMERLGYEEGLMALLTDPEKCHALLARFAAGA
                     SALATLQASRGVDAVLISSAFAGGGFISKAMYEGFVLPYERQVVEAIHAAGVKAYTHT
                     CGAIGDRLELMQATGIDGIDTMDPPPLGDTDLADAKQRVGDRLFLKGNLDSVNVLLRG
                     "
     gene            complement(1020..>1620)
                     /locus_tag="PLY56_18600"
     CDS             complement(1020..>1620)
                     /locus_tag="PLY56_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1559 domain-containing protein"
                     /protein_id="HOJ23541.1"
                     /translation="AAILFPVFARARENARKSNCLSNCKQLALAALAYAQDYDETLPK
                     ARNFATPTNYGLWTSVIHPYVKNGNIFRCPSRTTNGYTSTVVNPVTGIPLQQTGYGWN
                     IGTSDTSYHNGVGYYDGDDQPWVALAQIPAPAETIMLGDISYYTDNHIYLLYSTTAIS
                     YTPNLHTDGGNYAFVDGHVKFIPQREAHAQKRLFTIQED"
BASE COUNT          291 a          469 c          554 g          306 t
ORIGIN      
        1 gccacgcagg agcacgttga cggagtcgag gttgcccttc aggaagaggc ggtcaccgac
       61 gcgctgcttg gcatccgcga gatcggtatc tcccagcgga ggcgggtcca tcgtatcgat
      121 accgtcgatc ccggtggcct gcatcagctc cagccggtcg ccgatagcgc cgcaggtgtg
      181 cgtgtaggcc ttgacgcccg cggcgtggat tgcctccacc acctggcgct cgtagggcag
      241 gacgaacccc tcgtacatcg ccttcgagat gaagccgccg ccggcgaagg cggaggagat
      301 caacacggca tctaccccgc gcgaggcctg tagggtggcc agagcgctgg cgcccgcggc
      361 gaaccgcgca agaagcgcgt ggcacttctc cggatcggtc aacagcgcca tgaggccctc
      421 ctcatagccc agccgctcca tcaactgcgt gaagggcgag aagacctcac tgtggacact
      481 gagacgcttc cccacgcggg cgatgacgcc gtcgataatc gccgtaaggt agggcggaaa
      541 gaagtcgtcg ccggatccgg agaggggctc caggtcgtaa tggaagggat acttcaggcc
      601 gccgagggtg tgcgggtcct catagaagac ccgctcggga tcgatctctt cgagggcgag
      661 ccgtggagtc tccacctcat gagggagatc gtctggaggg cagaggcagc gcccgctacg
      721 cttccagtgg atcacttcgc cctcgggtgt ctgctcgatc cggtccacga agtcatcggc
      781 gttggggggg cgacccggga gattgatcag gattccatcg aagccgtagc gctcggcgag
      841 cgcgacgaag caatcgatga tcgtctcgtt atgcagccag tagttggccc cggagacgcc
      901 gcactgtagg aggtagtgcc cgatggagag ctggcacatc aaagggacgc ggtccggctg
      961 ccgccgctcc atcgcggcca cgatgcgttc tcgggaagtc atatccgtta ggttcctgtt
     1021 caatcctcct ggattgtgaa gaggcgcttc tgggcgtgcg cctcgcgctg cgggatgaac
     1081 ttcacgtgcc cgtccacgaa cgcgtagttg cccccgtcgg tgtgaaggtt tggggtgtag
     1141 cttatcgcgg tcgtcgaata gagcaagtag atgtggttgt cggtgtaata ggagatatcg
     1201 ccgagcatga tcgtctcggc cggcgccgga atctgcgcga gcgccaccca cggctggtca
     1261 tcgccgtcgt agtagcccac gccattatgg taactcgtgt cactcgtacc gatgttccag
     1321 ccatagccgg tctgctggag cgggataccg gtgacgggat tcacgacggt gctcgtatag
     1381 ccgttcgtgg ttcgggacgg gcatcggaag atgttcccgt tcttcacgta cgggtggatc
     1441 acggaggtcc agagcccgta gttcgtgggg gtagcgaagt tgcgcgcttt gggcagcgtt
     1501 tcgtcgtagt cctgggcata ggccagggcg gcgagagcga gctgcttaca gttggagagg
     1561 cagttgctct tgcgtgcgtt ctcgcgcgct cgcgcaaaga ctggaaaaag gattgccgcg
//
LOCUS       DAOSCV010001015         1617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51016_length_1617_cov_2.234955, whole genome shotgun sequence.
ACCESSION   DAOSCV010001015 DAOSCV010000000
VERSION     DAOSCV010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51016_length_1617_cov_2.234955"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..765
                     /locus_tag="PLY56_18605"
     CDS             394..765
                     /locus_tag="PLY56_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOJ23542.1"
                     /translation="MAREIVITAGAVSARATLLDNPTADAIWDALPLDAAASTWGDEI
                     YFSIPVHVDVQDGQEVVDLGDLGYWPPGSAFCIFFGPTPMSRGKEIRPASAVGVFGKV
                     TGDPKVFKKVRSGERVKIERA"
     gene            762..>1036
                     /gene="dut"
                     /locus_tag="PLY56_18610"
     CDS             762..>1036
                     /gene="dut"
                     /locus_tag="PLY56_18610"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOJ23543.1"
                     /translation="MIRVPVLLLPHGEGLPLPDYATEHSAGADLLAAIEGELRLAPGE
                     RALVPTGIAVAIPPGYEIQIRPRSGLAIRHGITCLNSPGTIDADYRG"
     assembly_gap    1037..1046
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1047..1290
                     /locus_tag="PLY56_18615"
     CDS             <1047..1290
                     /locus_tag="PLY56_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23544.1"
                     /translation="ICVILVNLGQEAFTLRRGDRIAQMVLAPVARIAWEPVQDLPATE
                     RGSGGFGHTGVTTPLEAGLRQQARAGSGSDLIEGTS"
     gene            1426..>1617
                     /locus_tag="PLY56_18620"
     CDS             1426..>1617
                     /locus_tag="PLY56_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23545.1"
                     /translation="MRPTAIRSLPEELPAPSEFLNPPPGYGPVPFWFWNARMEEAEVR
                     RQVREMAEKGCSGAFLHARH"
BASE COUNT          248 a          542 c          563 g          254 t
ORIGIN      
        1 cgccgacggg cacgcgaagt ggatcaagcc agaggtgctg tggaacggcg gcaaggacta
       61 cccctactac aagcagtggt agccgcctgg attctgggat gagtccgaac taccggcgct
      121 ggcgcgtccg gcgcctaacc ccccccggcc cattgcctac ctgccagccc ctcgctggcc
      181 ccagggaagg ggcgctaccg cgcggggggc gcggttgccc ccggacgggg cgcgagatcc
      241 gggggaatgc cggaacaggg cgaatgttca ctgttcgccc tgccagcccg gctcggccca
      301 tccgcggtat cgatgcacgc cggccccacg tgtgttgcac cgcctgcttg acacgcgcta
      361 gctcctcggg gtagaatggg cccggaggga acaatggcac gagagatcgt gatcaccgcg
      421 ggcgccgtca gcgcgcgcgc gactctgctg gacaacccca ccgcggatgc catctgggac
      481 gccctgcccc tggatgccgc ggccagcacc tggggggatg agatctactt ctcgatcccg
      541 gtgcatgtcg acgtgcagga tggccaggaa gtggtcgacc tgggcgatct gggctactgg
      601 cctcccggca gcgcgttttg cattttcttc gggcccacgc cgatgagccg aggcaaagag
      661 atccgcccgg ccagcgccgt cggcgtcttc ggcaaggtca cgggcgatcc caaggtcttc
      721 aagaaagtgc gcagtggcga gcgcgtgaag atcgagcggg catgatccgg gtccctgtgc
      781 ttctcttgcc gcacggggag ggcctgccgc ttccggacta cgccacggag cactcggctg
      841 gtgccgatct tctggcggcg attgagggcg agctgcgcct ggcgccaggc gagcgcgccc
      901 tcgtgccgac cggcatcgcc gttgccatcc ccccgggata cgagatccag atccgcccgc
      961 gcagcggact ggcgatccgc cacggcatca cctgcctcaa ctcgcctgga acgatcgacg
     1021 cggactaccg gggcgannnn nnnnnngatc tgcgtcatcc tggtcaacct cgggcaggag
     1081 gcgttcaccc tgcgccgggg cgaccggatc gcgcagatgg tgctggcgcc cgtcgcgcgg
     1141 attgcctggg agccggtcca ggatctcccg gccacggagc gcggctcggg cgggttcggg
     1201 catactggtg tcaccacccc cctggaggct ggcctgcggc agcaagcgcg ggcaggaagc
     1261 gggagtgatc ttatagaagg tacctcgtga ggcgttctcc gtggccggcg ggtgtttggg
     1321 tggtgccggc ccggcggtat ccggccggca ccaacgccgt gcgagcgcgg attccgtttc
     1381 acttctcacg cttcacgaga cgccatgcat ccaagagcag tcagcttgag gccaaccgcc
     1441 atccggtcgc ttccggagga gttgcccgcg cccagcgagt ttctcaatcc gccgccgggg
     1501 tacgggccag tgccgttctg gttctggaat gcccggatgg aggaggccga agtgcgccgc
     1561 caggtgcgcg agatggccga gaaaggctgc tccggcgcct tcctgcacgc ccggcac
//
LOCUS       DAOSCV010001016         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51048_length_1616_cov_2.716208, whole genome shotgun sequence.
ACCESSION   DAOSCV010001016 DAOSCV010000000
VERSION     DAOSCV010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51048_length_1616_cov_2.716208"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1234
                     /locus_tag="PLY56_18625"
     CDS             125..1234
                     /locus_tag="PLY56_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23546.1"
                     /translation="MISHSEPIRVGLIGYGGYGRFLIESWAEMPEIEIRAIAGRNRER
                     LERTARRLGIPRFYVGHEALLTDPEIDVVAVTSPPGDHARHAIDVAKAGKHLYCEKPL
                     ATRVEDGEAILDAVRTAGVRGVMGYVMRYTPLAERLRQIVAEGVLGALHRVDLANFAA
                     DECLPPDHWFWNRERSGGILVEHGVHFFDLYTWIAGSRPVRVTGHRTIRPGTTQEDRV
                     LATVEYESGLLATFYHAFDKPVRLERTWARLGFDRGYATLTGWIPLCLDVEAAVDEAE
                     YARLIALCPEASVTLHEEYPGPGEVIRGGGNSYRVTRLIEMRCQPTLDKQAVYAAAAR
                     ASLRDLVAAIRNPGHRVRAPLEAGLESLRIAASVE"
     gene            1387..>1616
                     /locus_tag="PLY56_18630"
     CDS             1387..>1616
                     /locus_tag="PLY56_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004594060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23547.1"
                     /translation="MKITVIGTGYVGLVQAVGLAELGHEVVGMDHDVAKVRREILVKE
                     SANSYGKVRADFLPLYRVKRGGRLYPPADRTL"
BASE COUNT          272 a          477 c          576 g          291 t
ORIGIN      
        1 ggagcttcga gctccgcgcg tgcgggcagc cttctcctta tctcggaatc ccaggtgcca
       61 ccgcatgttg gttgacttgg ctttagagcg caggttataa tgcgggagag ggaggatcgg
      121 gggcatgata agccactcag agcccattcg tgtcggcctg atcggctatg gcggatacgg
      181 ccgctttctg atcgagagct gggcggagat gccagagatc gagatccggg cgattgcggg
      241 tagaaaccgg gagcgcctgg agcggacggc gcgcaggctc ggcatccccc gcttctacgt
      301 gggccacgag gccctcctga ccgatccaga gattgacgtg gtcgcggtga ccagcccgcc
      361 gggagaccac gctcgccatg cgatcgatgt ggcgaaggcg ggaaagcacc tctactgcga
      421 gaagccactg gcaacgcgcg tggaagatgg cgaggcgatc ctcgacgcgg ttcgcacggc
      481 gggcgttcgc ggcgtgatgg gctacgtgat gcgctacacg ccgctggcgg agcgcttgcg
      541 gcagattgtg gccgaggggg tgctgggcgc gttgcatcgc gtcgatctgg ccaacttcgc
      601 ggcggacgag tgcctacccc cggatcactg gttctggaat cgggagcgga gcggcggcat
      661 cctggtggag cacggcgtgc actttttcga tctctacacg tggatcgccg ggagccggcc
      721 ggtgcgggtg accgggcacc gcaccatccg gccgggaacg acgcaagaag atcgcgttct
      781 ggcgaccgtg gagtatgaga gcgggctcct ggcgactttc taccatgcgt tcgataagcc
      841 ggtgcgcctc gagcggacgt gggcgcgtct cggttttgat cggggctatg cgacgctcac
      901 tggatggatc cctctctgcc tagatgtgga agcggccgta gacgaggccg aatatgcccg
      961 gctgatcgct ctctgccccg aagcctcggt cacgctccac gaggagtatc ctgggccggg
     1021 cgaggtgatc cgcgggggcg ggaacagcta tcgagtgacg cgcctgatcg agatgcgctg
     1081 ccagcccacg ctcgataagc aggcggtcta cgcggctgcc gcgcgcgcgt cgttgaggga
     1141 tctggtggcg gccattcgca atccggggca tcgggtgcgc gccccactgg aagcgggtct
     1201 ggagagcctg cgtattgccg cctctgtgga gtaggcccga ggggcacaca gcacaggcat
     1261 cctctcgtgg gagagcacct cttctccacc cagggcctct atcgtgccca ccggcgttgc
     1321 attacgtgct gggcagcggc cctgcgtggc gtttcatgac atctgttgag tatcaaggta
     1381 gatcgcatga agatcacggt tatcggcacg gggtatgtgg gcctggtcca ggcggttgga
     1441 ttggcagagc tcggacacga agtggtcggc atggaccatg acgtggccaa ggtgcgccgg
     1501 gaaatcctgg tcaaggaatc ggccaactca tacggcaagg tccgggctga cttcctgccc
     1561 ctctaccggg tcaagcgggg cggccgcctc tacccgcccg ctgaccgaac cctgaa
//
LOCUS       DAOSCV010001017         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51090_length_1615_cov_3.198718, whole genome shotgun sequence.
ACCESSION   DAOSCV010001017 DAOSCV010000000
VERSION     DAOSCV010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51090_length_1615_cov_3.198718"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1218
                     /locus_tag="PLY56_18635"
     CDS             187..1218
                     /locus_tag="PLY56_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007361714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23548.1"
                     /translation="MTRFNKPSEIKVGVIGYGGAFNMGKLHLTQMQAAGMTPTAVAEI
                     DPKRLEAAQADFPGIETYSSVAEMLEKSDVHLLAVITPHNTHAELALQCLNAGRHVVT
                     EKPFAITTAECDAMIAAAKENVVVLSTYHNRHWDGCVMEAVRQIRSGVIGEVFRIEAH
                     MANYAQPRDWWRSSKSISGGILYDWGVHLLEYSLQIIDSEMCEVTGLAKTGFWADKTR
                     WGADTNEDEGFGVVRFTNGKWLTLRISAIDSNPSGRWLEITGTQGTYLFDHTTYEIIK
                     HEEGKTHIIRGTNPPSEGQRFYDNIADHLTKGTPLVITPEWARRPIHILDLMNQSAAQ
                     GTALKTTYR"
     gene            complement(1297..>1615)
                     /locus_tag="PLY56_18640"
     CDS             complement(1297..>1615)
                     /locus_tag="PLY56_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23549.1"
                     /translation="SDEIPRKTREALAEAVRRRQALAQAEREVTDLTKQIADLRRDQA
                     ETRSNMGAVTRNSQAYERFEKKLLEIETRIEALQKELDAKRDAVKKLRQELDDYLAGL
                     NVE"
BASE COUNT          305 a          559 c          480 g          271 t
ORIGIN      
        1 cccgaggtgc ccctcgccaa tatcgatgcc ctcttcgagg cgtgtcggga atacggtacc
       61 tttgaatgac gcgtgtcgcg cgccagcgcg gaaaacaacc caccagatcc tgaaggggca
      121 gagatctctc aaccattctc cccgcgcccc agtggcgcgc tcatccaccg agaggagcaa
      181 acagacgtga ccaggttcaa caagccctcg gagatcaaag tgggcgttat cggctatggc
      241 ggcgccttca acatgggcaa gctgcacctc acccagatgc aggccgccgg catgacgcca
      301 accgccgtcg ccgagatcga cccgaaacgc ctcgaagccg cccaggcgga cttccctgga
      361 atcgagacct acagctcggt cgccgagatg ctggagaagt cggacgtcca cctgctggcg
      421 gtgatcaccc cgcacaacac ccatgccgag ctggcactcc agtgcctcaa cgccggccgg
      481 catgtggtca cagagaagcc gttcgcgatc accaccgccg aatgcgacgc gatgatcgcc
      541 gccgcgaagg agaacgtcgt cgtcctttcc acctaccaca accggcactg ggatggctgt
      601 gtgatggaag ccgtccgcca gatccgctcc ggcgtcatcg gcgaggtatt ccggatcgag
      661 gcgcacatgg ccaactatgc ccagccgcgc gattggtggc gctccagcaa atcgatctcc
      721 ggaggcatcc tctacgattg gggcgtgcac ctgctggagt actcgctcca gatcatcgat
      781 tcggagatgt gcgaggtgac cgggctcgcc aagaccgggt tctgggccga caagacccgc
      841 tggggagccg atacgaacga ggacgagggc ttcggcgtag tccggtttac caacggcaag
      901 tggctcacgc tacggatcag cgccattgat agcaacccct ctggccgctg gctggagatc
      961 accggcacgc agggcaccta tctcttcgat cacaccacct acgagattat caagcacgaa
     1021 gaggggaaga cccacatcat ccgcggcacc aacccgccga gtgagggcca gaggttctac
     1081 gataacatcg ccgaccacct caccaagggc acgcccctgg tgatcacccc cgagtgggca
     1141 cgccggccga tccatatcct cgacctgatg aatcagagcg ccgcgcaggg aacggcgctg
     1201 aagacgacct accggtagcg cccggcgctt ccggaaaacg cgcggggggc caggcgatgc
     1261 ctggcccccc ttcgtcgcat ccggacgcgg ccggttctac tccacgttca ggccggccag
     1321 atagtcgtcc agctcctggc gcagtttctt cacggcgtca cgcttcgcgt ccagctcctt
     1381 ctggagggcc tcgattcgcg tttcgatctc cagcagcttc ttttcgaacc gctcgtaggc
     1441 ctggctgttc cgggtcacgg cacccatgtt cgaccgcgtc tccgcctggt cgcgccgcag
     1501 gtcggcgatc tgcttggtca gatcggtcac ttcgcgttcc gcctgcgcga gcgcctggcg
     1561 ccgtcgaacc gcctcggcga gcgcctcgcg cgtcttccgc gggatctcgt cgctg
//
LOCUS       DAOSCV010001018         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51346_length_1610_cov_2.493248, whole genome shotgun sequence.
ACCESSION   DAOSCV010001018 DAOSCV010000000
VERSION     DAOSCV010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51346_length_1610_cov_2.493248"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLY56_18645"
     CDS             <1..755
                     /locus_tag="PLY56_18645"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOJ23550.1"
                     /translation="SEKLPYYHAVALALQSTWADQLTRLGDPTETAIALDRILSDEHT
                     TRMRHRIEHLVHTASVLHGPEVIGLPGGTVHIAVADPEGNLAALTLTHGNPFGSGLSV
                     PGGGTLVAGGMGYFERAPGRPNSVGPRTRPLYPACPTLVMKEECPLLLLGGIGGRKAA
                     SAVVQALLHYCALRHPVDDSLCLPRLHTDGSLTLCVDPGLPRPASDYLRAVGYLLESG
                     EAGALFALMRDPDSGLFTGVVDPRATGLAVGI"
     gene            complement(887..1609)
                     /locus_tag="PLY56_18650"
     CDS             complement(887..1609)
                     /locus_tag="PLY56_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ23551.1"
                     /translation="MEHLRKEFGNLVAVEDLCLRIPPGEFFGFLGPNGSGKTTTIKLL
                     TGLLRPTRGVARIGGFDIQKEHLAAKRLIGYVPDTPYLYEKLSAREFLRLVGDLYQIP
                     VQTLDRRITELLELLGLAHVADGLIEDYSHGMRQKAVMCACLLHEPKAIIIDEPMVGL
                     DPQSAHLVKTVLRKQCEQGACVFMSTHTLSLAEDVCHRVAIILKGRVIAVGTVDELKR
                     EYGGHSDLEDLFLDLTGRDVGA"
BASE COUNT          276 a          537 c          521 g          276 t
ORIGIN      
        1 ggagcgaaaa gcttccctac taccacgcgg tggcgctcgc tctgcaatcc acatgggccg
       61 atcagctcac ccgtctagga gatcccacgg agacggccat cgccctggac cgcatccttt
      121 ccgacgagca cacgacccgg atgcgccatc ggatcgagca tctcgttcac acggccagcg
      181 tgttgcacgg cccggaggtc atcggtctcc cgggaggcac cgtgcatatc gccgtcgcgg
      241 atccggaggg caacctggcc gcgctgacgc tgacacacgg caacccattc ggttccgggc
      301 tctccgtgcc gggcggcggc acgctcgtgg cgggaggaat gggctatttc gaacgggcgc
      361 cgggccggcc caactccgtg gggccgcgaa cacgcccgct ctatccggcg tgccccacgc
      421 tggtcatgaa ggaggagtgc cccctcctgc tactcggggg gatcggcggg cgcaaggccg
      481 ccagcgccgt ggtgcaggca ctgctccact actgcgcgct ccggcatccg gtcgacgact
      541 ctctctgcct gccccgcctg catacggacg gctctctcac gctctgcgtg gatcccgggc
      601 tgccgcggcc cgcgagtgac tacctgcgcg ccgtcggcta cctgctggaa tctggcgagg
      661 cgggcgcgct ctttgccctg atgcgcgatc ccgactcggg gcttttcacg ggcgtcgtag
      721 accctcgcgc aaccggcctg gccgtgggaa tctagtatct aatcgcgaga ggggcaaccc
      781 ggggcctggt gaacctcgaa gaacaaacca gctatgccgg tggaggcatt gcctacacgc
      841 ggcatcatcc tgaagctggt caatccagtg tcatctgcgg attccatcac gcgccgacgt
      901 cacgtccggt cagatcgagg aagaggtctt ccaggtcgct gtgaccgccg tattcgcgct
      961 tcagctcgtc cacggtgccc acggcaatca cgcggccctt gagaatgata gcgacgcggt
     1021 ggcagacatc ctcggccagg gagagcgtgt gggtggacat gaaaacgcac gccccctgct
     1081 cgcactgctt gcggaggacg gtcttgacca ggtgggcgct ctgcggatcg agcccgacca
     1141 tcggctcatc gatgatgatc gccttcggct cgtggaggag gcaggcgcac atcaccgcct
     1201 tctggcgcat tccgtgagag taatcctcaa tcaggccgtc ggccacgtga gcgagcccaa
     1261 gcagctccag cagctcggtg attcgccggt caagtgtctg gaccggaatc tggtagagat
     1321 caccgacgag acggagaaac tcgcgcgcag agagcttctc gtagaggtag ggggtatcgg
     1381 gcacgtagcc gatcaggcgc ttggctgcga ggtgctcctt ttggatatcg aatcccccga
     1441 tgcgggccac gccgcgcgtg gggcgcagca gcccagtgag gagcttgatg gtcgtcgtct
     1501 tccccgagcc gttgggcccc agaaacccga agaactcgcc cggcgggatc cgcaggcaga
     1561 gatcctctac cgcgaccagg ttgccgaact ccttgcgcag atgctcgatc
//
LOCUS       DAOSCV010001019         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51466_length_1607_cov_6.882088, whole genome shotgun sequence.
ACCESSION   DAOSCV010001019 DAOSCV010000000
VERSION     DAOSCV010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51466_length_1607_cov_6.882088"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1607
                     /locus_tag="PLY56_18655"
     CDS             <1..>1607
                     /locus_tag="PLY56_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOJ23552.1"
                     /translation="EDPAAEGFDPDEGTVARGRGKCLACSETVPLEAIHRQGQAGRIG
                     QILVSLVVRRDGRREYRTAGRQDLEAVAAAEQVLEARVFEYANQGLLPDEDLPLGYTT
                     EQPKRYGILAWRQMFTPRQLLSHVVHLKHIRQVGEEMAREFKAEQDPAGRYRWEAVMT
                     YLALLFDKTVDYNSRMTRWAADRAILKGSFDRHDYAFKWSFGEAVPVYENWPWATNQI
                     LDAYRQLAKLAAPYRLFAPMEGMQRNLGSSAGAGKAGGESEAAGPPATQESPPVHVTQ
                     GNAATYYALPDGSVDIIVVDPPYYDNVMYAECSDFFYVWQKRALGHVFPELFGATLTD
                     KQQETVANVAQFNGRKGTKELAQQDYEDKMRAAFRRMHGLLRDDGVMVVMFTHKQTEA
                     WDSLASALIEGGWEITASWPVHTESEHSLHQAKKNAAQSTILLVCRKREAGAEGEGGW
                     WQQVQGEITRQVSERVAEYEALGVRGVDLTLATFGPALQVICRHWPVRLASGEVITPE
                     YALDLARKVVQEHRWVQLVGARPVEVD"
BASE COUNT          309 a          444 c          615 g          239 t
ORIGIN      
        1 tcgaggaccc ggcggcggag ggcttcgacc cggacgaggg gaccgtggcg cgaggacgcg
       61 ggaagtgcct tgcctgctca gagacggtac ctcttgaggc gatccaccgg caaggacagg
      121 cgggcaggat cgggcagatc ctagtgagtc tggtcgtgcg acgcgacgga aggcgcgagt
      181 acaggacagc aggccgacag gacctagagg ccgttgccgc agcagagcag gtgctggaag
      241 cgcgtgtctt cgagtacgcg aaccaagggc ttcttccgga cgaggacttg cccctcggct
      301 acacgaccga gcagccaaag cggtatggaa tactggcgtg gcggcagatg ttcacgcccc
      361 ggcagcttct gagccacgtg gtgcatttga aacatatccg ccaggtcggc gaggagatgg
      421 cgcgggagtt caaggctgag caggacccag ccggccgata ccgctgggaa gcggtgatga
      481 cgtacctggc gctcctgttc gacaagacgg tggactacaa ctcgcggatg acgaggtggg
      541 cagctgaccg agccatactg aaggggtcgt tcgaccgcca cgactacgcg ttcaagtgga
      601 gcttcgggga agccgtgccc gtctacgaga actggccttg ggcgactaat cagattctcg
      661 acgcctaccg ccagctcgcc aaactggcgg ctccctaccg gctgttcgcg cccatggagg
      721 ggatgcagcg caaccttggg agcagcgcgg gggcagggaa ggcaggcggg gagtcggagg
      781 cggccggccc gccggccacg caggaatctc cccctgtgca cgtcacccag ggcaatgcgg
      841 ccacctacta cgccctgccc gacgggtccg tggacatcat cgtggtggac ccaccctact
      901 acgacaacgt gatgtacgcc gagtgctcgg acttcttcta cgtctggcag aagcgggcgc
      961 ttgggcatgt attcccggag ctgttcggtg cgacgctgac ggacaagcag caggaaacgg
     1021 tggcgaacgt cgcccagttc aacgggcgca agggcacgaa ggagctggcg cagcaggact
     1081 acgaggacaa gatgcgggcg gccttccggc ggatgcacgg gctgctgagg gacgacggcg
     1141 tgatggtggt gatgttcacc cacaagcaga cggaggcctg ggattcgctg gcgtcggcct
     1201 tgatcgaggg cggatgggag ataacggcat cgtggccggt acacacggag agcgagcaca
     1261 gtctgcacca ggcgaagaag aatgcggcac agagcacaat cctgctggtg tgccgcaagc
     1321 gtgaggccgg cgcggagggc gagggtggct ggtggcagca ggtgcagggg gagatcacgc
     1381 ggcaggtgag cgagcgggtg gcagagtacg aggcgctagg agtgcggggt gtggacctga
     1441 cgctggcgac ctttgggccg gcgctgcagg tgatttgccg tcactggccg gtgcggctgg
     1501 cgtcggggga ggtgatcaca ccggagtacg cgctggattt ggcgcggaag gtggtgcagg
     1561 aacaccgctg ggtgcagctt gtgggggcgc ggccggtgga agtggac
//
LOCUS       DAOSCV010001020         1598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51920_length_1598_cov_3.217758, whole genome shotgun sequence.
ACCESSION   DAOSCV010001020 DAOSCV010000000
VERSION     DAOSCV010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51920_length_1598_cov_3.217758"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PLY56_18660"
     CDS             <1..687
                     /locus_tag="PLY56_18660"
                     /inference="COORDINATES: protein motif:HMM:NF024819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HOJ23553.1"
                     /translation="ERVASGRADFALAAIRADTPELQAEPFCQDGFHLVCRADHPLAT
                     RLDGIYLPLAELGKSNPELPPYPYCDAGAFWASGHWDANAVTWWAVKHRGITVAGPPP
                     RALPFDASWDGVERAMGHNLTVVWPREVGRRSAFARDADVQFAVLTLCRIHATLAQGA
                     IVSKRAGAEHALASLPSRWHRLIEEAVRLRDGAGEPSCFQSRDERARETRQFVEWMIT
                     EYQAGRLAKE"
     gene            693..>1598
                     /locus_tag="PLY56_18665"
     CDS             693..>1598
                     /locus_tag="PLY56_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007431297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOJ23554.1"
                     /translation="MPREYKPSEIEPKWQRYWEEHKTFRAVDFDPKPKYYILDMFPYP
                     SGAGLHVGHPEGYTATDILARYKRMRGFNVLHPMGWDAFGLPAEQYAIDTGTHPAITT
                     RKNIETFKRQIKSLGFSYDWDREVNTTDPGYYKWTQWIFLQLYKRGLAYVAEVPVNWC
                     PALGTVLANEEVIDGRSERGGHPVIRKPMRQWMLKITAYADRLLEDLDLVEWPESIKE
                     MQRNWIGRSIGAEVEFPVQGHNANIRVFTTRPDTLFGATYMVLAPEHPLVDVVTTPEQ
                     RQAVQARIDARLAAAAERVIERPDED"
BASE COUNT          294 a          517 c          532 g          255 t
ORIGIN      
        1 gagcgcgtgg ccagcggccg ggccgacttt gccctggcgg ccattcgcgc cgacacgccc
       61 gaactgcagg ccgaaccctt ctgccaggac ggttttcacc tggtctgccg cgcggatcac
      121 ccgcttgcca cccggctgga tgggatctac ctcccgctgg cggagctggg gaaaagcaac
      181 cccgagttgc ccccctaccc ctattgcgac gccggagcgt tttgggccag tggccattgg
      241 gacgcgaacg ccgtcacctg gtgggcagtc aaacaccgcg gcatcaccgt ggccgggcct
      301 cccccgcggg cactgccgtt cgatgcgagt tgggatggcg tcgagcgagc catggggcac
      361 aacctcaccg tcgtctggcc gcgggaagtc ggcagacgct ccgccttcgc gcgagatgcc
      421 gacgtacagt tcgcggtgtt gacgctatgc cggatccacg ccacgctggc ccagggcgcc
      481 attgtctcga agcgcgcggg cgcagagcat gcgctggcga gcctgccctc gcgctggcac
      541 cggctgattg aggaggcggt gcgcctccgc gatggcgcag gcgagccgtc gtgcttccag
      601 tcgcgtgatg aacgagcgcg tgagacccgg cagtttgttg agtggatgat cacagagtat
      661 caggcgggac gcctcgccaa ggagtagccc taatgcccag agagtacaaa ccatcagaga
      721 tcgaacccaa gtggcagcgc tattgggaag agcataagac gttccgggct gtggattttg
      781 acccgaagcc gaagtactac atcctggata tgttccccta cccctctggc gccggcctgc
      841 acgtcggcca tcccgagggg tatacggcca cggatatcct ggcgcgctac aagcggatga
      901 gggggttcaa cgttctgcac ccgatgggct gggatgcatt tggcctgccc gccgagcagt
      961 acgccatcga tacgggcaca caccccgcaa tcaccacccg gaagaacatc gagacgttca
     1021 agcggcagat caagtcgctg ggcttctcct acgactggga ccgcgaggtg aacaccaccg
     1081 acccgggcta ctacaagtgg acgcagtgga tcttcctgca actgtataag cgcgggctgg
     1141 cctacgtggc ggaggtgccc gtgaactggt gcccggctct gggcacggtg ctggccaacg
     1201 aagaggtgat cgacgggcgc agcgagcgcg gcggacaccc cgtcatccgc aagccgatgc
     1261 gccagtggat gctcaagatc accgcctatg ccgaccgcct gctggaggat ctggacctgg
     1321 tggagtggcc ggagagcatc aaggagatgc agcgcaactg gatcggccgc tccatcggcg
     1381 cggaggtgga gttcccggtg caggggcaca acgccaacat ccgtgtcttc accacccgcc
     1441 cggatacgct gtttggcgcc acctacatgg tgctggcgcc cgaacatccg ctggtggacg
     1501 tggtcaccac acccgagcag cgacaggcgg tgcaggcccg gatcgacgcc cggctcgcgg
     1561 cggccgcgga acgtgtaata gagcgtccgg acgaagat
//
LOCUS       DAOSCV010001021         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51963_length_1597_cov_4.370947, whole genome shotgun sequence.
ACCESSION   DAOSCV010001021 DAOSCV010000000
VERSION     DAOSCV010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51963_length_1597_cov_4.370947"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLY56_18670"
     CDS             complement(<1..202)
                     /locus_tag="PLY56_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ23555.1"
                     /translation="MGFELQLAIRHLRAGGWQTVLIMAGVAIAVTLVIFVSGLIYGVQ
                     ERWLGILTGSIAHVTVTAPEPEP"
     gene            complement(239..>1597)
                     /locus_tag="PLY56_18675"
     CDS             complement(239..>1597)
                     /locus_tag="PLY56_18675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ23556.1"
                     /translation="TPASEIEQARAEVQARVAEARARLMRAEERLRELLAGGTAAQRQ
                     EAQAQVREAEARVAQARRDVEQAEALARSDDTARAALEQAQATLRAAETGVERARIAL
                     EEAERDQARYERLYQQGAVPRIQVEERRAAVRTAREALEHANAERELAEVDVTRQRRL
                     LAVTREAELQQARAALQTAEEALEAARARLRAIREPARAETIAQQRAEVAAAREALAQ
                     TRAAGRAQLETLTSRPRPEELAVAARRLQEAIRARDAAQAVLDAVQVKAPFSGVVTEV
                     LNEPGSAVGPGQPILRLTQIQNPEVRVEIDERNLSLIRAGQQVVLTADAYPDRSFSGR
                     VARVGARADPQRGTVEVVVQPLSTPGWLRSGLTVDANIIVAQRQQRLVVPTTAILRRG
                     DRALVLVVENGIVKERRVVLGQVGGEKAVILGGLRESEYVVIQSTSVQAGQRARPLIR
                     GE"
BASE COUNT          216 a          631 c          510 g          240 t
ORIGIN      
        1 gtggctctgg ctccggggcg gtcaccgtca cgtgcgcgat cgacccggtg agaatcccaa
       61 gccagcgctc ctgcacgcca tagatcaggc cgctcacgaa gatgacgagg gtgaccgcga
      121 tggcgacgcc ggccatgatc aagacggtct gccatccccc cgcgcgcaga tgccggatcg
      181 cgagctgtag ctcgaacccc atgccatccc tccccgacgg catcggcccc gtgggtcgtt
      241 actccccacg gatgagaggc cgcgcccgct gccccgcctg aacgctggtc gattggatca
      301 cgacgtattc gctctcgcgc agcccgccca ggatgaccgc cttctcgcct ccgacctggc
      361 ccagaacaac ccggcgctcc ttgacaatcc cattctcgac caccaggacg agcgcgcggt
      421 cgccccggcg caggattgcg gtcgtgggca ccacaagccg ctgttggcgc tgggccacga
      481 tgatgttggc atccaccgtc agcccggagc gcaaccatcc gggagtggag agtggctgga
      541 ccaccacttc cacggtgccc cgctgggggt ccgcgcgcgc cccgacgcgc gcgacacggc
      601 cagagaacga gcgatccgga taggcatccg cggtcaggac aacctgttgg cccgcgcgga
      661 taagcgacag gttccgctca tctatctcca cgcgcacctc tggattctgg atctgggtga
      721 ggcgcaggat cggctgcccc gggccgaccg ccgatccggg ctcgttgagc acctcggtga
      781 ccacgccgga aaagggggcc ttcacctgaa cggcatcgag cactgcctgc gcggcgtcgc
      841 gcgcgcgaat cgcctcttgc aggcggcgcg ccgccactgc gagctcctcc gggcgcgggc
      901 gcgaggtgag ggtttccagt tgcgcccggc cagccgcgcg cgtctgcgcc agcgcctcgc
      961 gcgcagctgc cacctcggcg cgctgctggg cgatcgtctc ggcacgggcc ggctctcgga
     1021 tcgcgcgtag cctggctcgg gcggcctcga gcgcctcctc ggcggtctga agcgctgccc
     1081 gagcctgttg gagctcggct tcgcgagtca ccgccaggag acgccgctgc cgtgtgacat
     1141 ccacctcggc cagctcccgt tccgcgttcg cgtgctccaa cgcctcgcgc gccgtgcgca
     1201 cagccgcgcg cctctcttcc acctggatac gcggcacggc accttgctga tagagccgct
     1261 cgtagcgcgc ctgatcgcgc tccgcctcct ccagggcaat ccgcgcacgc tcaacgcccg
     1321 tctccgcggc gcgaagggtg gcctgcgcct gctccagtgc cgcgcgcgcc gtatcatccg
     1381 atcgggcgag cgcctccgcc tgctctacat cgcggcgcgc ctgcgcgaca cgcgcctccg
     1441 cttcgcgcac ctgggcctgc gcttcctgcc gctgggccgc ggtgcccccc gccagcagct
     1501 cccgcaaccg ctcttccgcg cgcatcagcc gcgcccgcgc ctccgccacc cgcgcctgca
     1561 cctccgcgcg cgcctgctcg atctccgaag caggagt
//
LOCUS       DAOSCV010001022         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_51967_length_1597_cov_3.217899, whole genome shotgun sequence.
ACCESSION   DAOSCV010001022 DAOSCV010000000
VERSION     DAOSCV010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51967_length_1597_cov_3.217899"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLY56_18680"
     CDS             <1..295
                     /locus_tag="PLY56_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23557.1"
                     /translation="PYDGLARRIASKLGFTEWQVERWIDMLHDGDFSDVDDPTDEQKE
                     MVISAYQEYLQGDGPPPKSLHVVLGERFGLVPRQIHKTLVEYRLDRRREAFGF"
     gene            complement(433..1281)
                     /locus_tag="PLY56_18685"
     CDS             complement(433..1281)
                     /locus_tag="PLY56_18685"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ23558.1"
                     /translation="MQRADLHTHTTFSDGTLTPAEVVAHARELGLAAVGITDHDAIDG
                     VAEALEAGERLGVTVVPGVEVNTDVPGSEVHMLGYFIDIDSSTLREALKGLREGRLRR
                     AEQIVTKLRGVGVPVELERVLAIADGVGAVGRPHIARAIVEAGFAADISDAFDRYLSP
                     GRPAHVPRPRFTPEEAIVAIREGGGAPVLAHPASAGRDECLRDWMRLGLVGIEADHTS
                     HRPDEVLHYYRIARELGLIATAGSDFHGSPGTGAPLGERTCGLEVIDRLREAAERIRA
                     GLAAAP"
     gene            1405..>1597
                     /locus_tag="PLY56_18690"
     CDS             1405..>1597
                     /locus_tag="PLY56_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23559.1"
                     /translation="MAAMNSDSCQGCARARNEDGFAVPGSPECLGCRRAFTHRPDLPD
                     LWSGHPPGGADIRCDARGIT"
BASE COUNT          253 a          530 c          544 g          270 t
ORIGIN      
        1 gccgtatgat ggcctggccc gccggattgc ctccaaactc gggttcaccg aatggcaggt
       61 ggagcgctgg atcgacatgc tccatgacgg cgacttcagc gatgtcgacg atcccacgga
      121 cgagcagaag gagatggtga tctccgccta tcaggagtat ctccagggcg acgggccgcc
      181 accgaaatcc ctccacgtgg tgctgggtga gcggtttggg ctcgtgcctc gccagatcca
      241 caagacgctc gtagagtacc gcctggaccg ccgtcgggag gcgttcggct tctaggccgc
      301 ggcccaccca ataggagagc tatcagaggg tgcctgatcc cgggatcggg accatttctg
      361 gtgtgtgaac gtccggggca gagggagtgt gccgcgccgg cggtgtgcgc gacgcggcgt
      421 ctcgatggga tgctacgggg cggctgccaa gccagcgcgg atccgttccg ccgcctcgcg
      481 caggcgatca atcacctcca ggccgcaggt gcgctcgccg agcggggcgc cggtgcctgg
      541 ggagccgtgg aaatccgagc ctgctgtcgc gatgagcccc agttctcgcg caatgcggta
      601 gtagtggagc acctcatcgg ggcggtggct ggtatgatcc gcctcgattc ccaccaggcc
      661 gaggcgcatc cagtcgcgga ggcactcatc gcgcccggcc gatgccggat gtgccagcac
      721 aggtgcgccc cctccctcgc ggatcgccac gattgcctct tccggcgtga agcgtggccg
      781 cggcacgtgg gccggccggc ccggagagag gtaacgatca aaggcgtcgc tgatgtctgc
      841 cgcgaagccg gcctccacaa tggcacgcgc gatgtgcggc cggcccacgg cgcccacgcc
      901 atcggcaatc gcgagaacgc gctcgagctc cacgggcacg cccaccccgc gcagcttcgt
      961 cacgatctgc tccgcccggc gcagccgccc ctcccgcagg cccttcagcg cttcgcgcag
     1021 cgtgctcgaa tcgatgtcaa tgaagtagcc gagcatgtgc acctcggagc ccggcacgtc
     1081 ggtgttcacc tcgacgcctg gaacgacggt tacgcccagg cgctcgccgg cctccaatgc
     1141 ctcggcaacg ccgtcgatgg cgtcatggtc ggtgatcccc acggcggcaa gaccaagctc
     1201 gcgggcatgt gcgacgactt cggcgggggt cagcgtgcca tccgaaaagg ttgtgtgcgt
     1261 atgtagatct gctctctgca tctgtggtcc ctaacttcgc tgctcacttt ccctcgcggt
     1321 gccgtggcaa accgctccgc gatggcggca attcaccgcg ccggggtaca gtatggagcc
     1381 ggacgcggta taatagagcg agagatggct gccatgaact cggactcatg ccaggggtgc
     1441 gctcgcgcgc gcaatgagga tgggtttgcg gtgcccggct ccccagagtg cttgggctgt
     1501 cggcgtgcct tcacgcatcg cccggacttg ccggacctgt ggagtggtca cccgccgggt
     1561 ggggccgata tccgttgcga tgcgcgcggg atcacct
//
LOCUS       DAOSCV010001023         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52059_length_1595_cov_8.015584, whole genome shotgun sequence.
ACCESSION   DAOSCV010001023 DAOSCV010000000
VERSION     DAOSCV010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52059_length_1595_cov_8.015584"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..727
                     /locus_tag="PLY56_18695"
     CDS             296..727
                     /locus_tag="PLY56_18695"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ23560.1"
                     /translation="MQTDGVVYPRAQATVPPREAELASHHEDAEALAERFQALADASR
                     LKVLHLLYSRGEMCACELQSVLGVSASNLSFHLQVLRHAGFVKSRRVGKWTVYRLSPG
                     MPNGFLSAVAMRFAIRSGFSSANREEAPACCLCEAGESEES"
     gene            894..>1595
                     /locus_tag="PLY56_18700"
     CDS             894..>1595
                     /locus_tag="PLY56_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23561.1"
                     /translation="MRISLTTSSQAVHRAWNAVLAGLLLVLSVLLVAHVIHLSQGPRA
                     RGAGASSVAMLDPALSHLASPPGPPVTADRRVVLVTPVHPAPNNAEARERRWALARLS
                     RMLDRLGIPYASLPDTELSLAALRPYAVAILPGNVLTQGNTQSLEAYVAAGGRILALC
                     PRRRRQKACRARRAREGVRGPASPRQFRTLLFREPDQVLGLPEQMEQNSPHALILTPA
                     EGTRTLGWWHSRGETT"
BASE COUNT          249 a          542 c          509 g          295 t
ORIGIN      
        1 tcggggcttc tcatcccagc caccgccgtt ttgagacatc tgggagtatc cccaacccag
       61 cccggcaggg cttcgctcgc aagcagcgcg gtgatttatc gccgcgcgcc gggttgcgcg
      121 ctgcccccgt ggcttcggat tccttaatgt cggaatctca ggcgtagctt cgccgtcatg
      181 gacgttccgc agacggtatg gtatacttca accaggctga gagtgtggga ccgcgcgagc
      241 ctctcgacgc gcgcacctca gagacgcggc ttcacactcg tggaagggag aaagcatgca
      301 aacggatgga gtcgtgtatc cccgcgctca ggcgaccgtt ccaccgcgag aggccgagct
      361 ggcgagccac cacgaggatg ccgaggcgct ggccgaacgc tttcaggcac tcgcggatgc
      421 cagccgcttg aaagtgctcc acctcctcta ttcgcgtggc gagatgtgcg cctgcgagct
      481 tcagagcgtg ctcggggttt cagcatccaa cctctccttc cacctccagg tgctgcgcca
      541 tgctggcttc gtcaagtcgc gccgcgtcgg aaagtggact gtctaccgcc tttctcccgg
      601 aatgccgaac ggcttcctct ccgccgtggc aatgcgcttc gccatcaggt cgggcttctc
      661 ctccgcgaac agggaagaag cccccgcctg ctgcctctgc gaagctggag agagcgagga
      721 gtcttgagaa ccccggggca aaagcccgcg ggagagtgcc cccggcgcgg gcaggcgcgt
      781 gtcgggcgtt ggaacgattt gcgaagaaag gaggggtcgc cctctgcggg cgactggcgt
      841 cttgatccca ccatctgggc gcgtgggctt cgggcgccac gaattctgtg atcgtgcgca
      901 tttcattgac gacttcatcc caggcggtgc atcgcgcctg gaacgccgtg ctggccggat
      961 tgctgctcgt actcagcgtg cttctggtcg cgcatgtgat ccatctgagc cagggccccc
     1021 gagctcgtgg cgcgggcgct agttcagtgg cgatgctgga ccctgccctc tcacatctgg
     1081 catcccctcc aggccctcct gtgaccgcgg atcgccgcgt cgttcttgtc acgccggtcc
     1141 accccgctcc caataacgcg gaggcacgcg agcgccgctg ggcgctggcc cggctatcgc
     1201 gtatgctcga tcggctcggc atcccttacg cctctctccc ggatactgag ctttcgctgg
     1261 ccgctctgcg cccttacgcc gtggctatcc tccccggaaa tgtgctcacc caggggaaca
     1321 cccagtcgtt ggaggcctat gtggccgcag gcgggcgcat cctggcgctc tgccctcgca
     1381 ggagacgcca aaaagcgtgt agagcgcgtc gggcccgcga gggcgttcgg ggaccggcct
     1441 cgccgcggca gttccgaacg ctcctcttcc gagagcccga ccaggtgctg ggcctgcccg
     1501 agcagatgga gcaaaactcg ccgcatgcgc tgatcctcac accagcggaa ggcacgcgca
     1561 cgctcgggtg gtggcactct cgtggcgaga cgacg
//
LOCUS       DAOSCV010001024         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52095_length_1595_cov_1.992208, whole genome shotgun sequence.
ACCESSION   DAOSCV010001024 DAOSCV010000000
VERSION     DAOSCV010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52095_length_1595_cov_1.992208"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1595
                     /locus_tag="PLY56_18705"
     CDS             <1..>1595
                     /locus_tag="PLY56_18705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ23562.1"
                     /translation="RREVVDPPPDAPARPMSGVFRNRLIRGDNKLAMAALLPEFRGRV
                     NLVYIDPPFAAGADFRADVALGEDPAIIRKARSAPEAIAYRDAWGTGADSYPQMMYER
                     LVLIRELLSPDGSLYIHCDWRANAMLRLICDEIFGVECFQREIIWRIGWVSGYKAAAA
                     NWIRNHDTILFYTRDPTRFTFNKEYLPYPPGYRRRDGSPPRGPGYPLEDTWNCSEMDR
                     RYSIQIKSFSREKVGYETQKNEALLRRIIRASSNEGDLVADFFCGSGTTLAVAEELGR
                     RWIGCDIGRHALHIARKRLIDRQRSLTAKGSPCQPFDTFDLEDAERCWWFSHRLSASE
                     ENYRRTLLEHLGATPLASSPVQSESGEEKPLPGSGVLHGQRGDELVHIAPPGARFSRA
                     DLEQALAAARGRGGNRLVCLAWEFEPDLVRRAEWHGNARLLVIPPEAMEPNRREVVFF
                     EPGWLDVEICWREPFCADVRLTGFRPCLPERGDPELRARAAVAPFDLLDFWAIDFDHD
                     PETALFRHHWQSYRTRNNRRLVL"
BASE COUNT          257 a          560 c          504 g          274 t
ORIGIN      
        1 ccgccgcgaa gtggtcgacc cgccccccga cgctcccgct cgcccgatga gcggggtgtt
       61 ccgaaaccgc ctcatccggg gtgacaacaa gctggcgatg gccgctctcc tcccagagtt
      121 tcgcgggcgc gtgaatctgg tttacatcga cccgcccttc gccgccggcg ccgacttccg
      181 agcagacgtc gcccttggcg aggatccggc aatcatccga aaggcgcggt ccgcccccga
      241 agctatcgcc taccgggatg cctggggaac cggcgccgac tcctatcccc agatgatgta
      301 cgagcgtctc gtgctgattc gagagctgct ctcgccggat ggcagcctct atattcactg
      361 tgactggcgt gccaacgcca tgttgcgcct catctgtgac gagatcttcg gcgtcgagtg
      421 cttccagcgc gagatcatct ggcggatcgg ctgggtctcc ggctacaagg cggccgcggc
      481 caactggatc cgaaaccacg acaccatcct cttctacacg cgcgacccaa cccggttcac
      541 cttcaacaag gagtatctcc cctacccgcc cggctatcgt cggcgggatg gctcgccccc
      601 gcgcgggccg gggtatcccc tggaggacac ctggaactgc agcgagatgg accggcgcta
      661 ctccatccag attaagagct tctcgcgcga gaaggtaggc tacgaaacgc agaagaacga
      721 ggcgctcctg cgccgcatca tccgcgcctc ctcaaacgag ggagacctgg tagccgactt
      781 cttctgcgga tcgggcacca cgcttgccgt tgccgaggag ctgggccggc gctggatcgg
      841 gtgcgacatc gggcggcacg cgctccacat cgcgcgcaag cgcctgattg accggcagcg
      901 cagcctgacg gccaaaggat cgccctgcca acccttcgac accttcgatc tggaggacgc
      961 cgagcgatgc tggtggttct ctcaccggct ttccgcgagt gaggagaact accgccgcac
     1021 cctgctggag cacctgggag ccactcccct ggcctcttcc ccggtgcaga gtgaatccgg
     1081 cgaagagaaa cccctgcccg ggtcaggtgt cttgcatggg cagcgcgggg acgagctggt
     1141 gcacattgca cccccggggg cccgcttcag ccgcgccgat ctggaacagg cgctcgccgc
     1201 cgcgcgtgga cgaggcggca atcgcctcgt ctgcctggcc tgggagttcg agcccgactt
     1261 ggtgcgccgg gcagagtggc atggcaatgc ccgcctcctc gtgatccctc ccgaggcgat
     1321 ggagcccaac cggcgggagg tggtcttctt cgagccgggc tggctggatg tggagatctg
     1381 ctggcgcgag cccttctgcg ccgacgtgcg cctcaccggc tttcggccct gcctgccaga
     1441 gaggggcgac cccgagctgc gcgcccgagc cgcggtggcg ccgttcgacc tgctcgactt
     1501 ctgggccatt gactttgacc acgacccgga gaccgctctt ttccgtcacc actggcagtc
     1561 ctatcgcacg cgaaataacc ggcgcctggt gttgg
//
LOCUS       DAOSCV010001025         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52333_length_1590_cov_3.029967, whole genome shotgun sequence.
ACCESSION   DAOSCV010001025 DAOSCV010000000
VERSION     DAOSCV010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52333_length_1590_cov_3.029967"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLY56_18710"
     CDS             <1..551
                     /locus_tag="PLY56_18710"
                     /inference="COORDINATES: protein motif:HMM:NF041155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOJ23563.1"
                     /translation="SQLHLPLDVSPAAASAAFCARTEDNLIFNGHKANGHEYAGLTNA
                     PGRNAIAMRDWSETGNAFRDVVEATQMLVSNEFYGPFALVVSPALYAGLNRIHDGTGV
                     LELEQVRKIATAGVYQTPVLGNNVAVVLSVGSENMDLAVAQDLITAFLETSAMNHRFR
                     VFEVLALRIKRPGAICTIERAE"
     gene            complement(661..969)
                     /locus_tag="PLY56_18715"
     CDS             complement(661..969)
                     /locus_tag="PLY56_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23564.1"
                     /translation="MQITETPCPWCHKGRVIIHAADEPWIVKEDTGEIGRRGPKGRPA
                     RFRTSPVDWFSCTECAMNGTVSYLRMLLSGGVRRYLQEAALRQRATIRPCGGESVRPD
                     "
BASE COUNT          270 a          569 c          477 g          274 t
ORIGIN      
        1 cctctcagct tcacctgccg ttggatgtga gccctgccgc ggcgtccgcc gctttctgcg
       61 cccgaaccga ggataacctg atcttcaacg ggcacaaagc caatgggcac gagtacgccg
      121 gcctgacgaa cgcgccgggg cgcaacgcca tcgccatgcg cgactggtcg gagacaggga
      181 acgccttccg agacgtcgtc gaggccacgc agatgctggt gtccaacgag ttctatggcc
      241 ccttcgcgct ggtggtgagc ccggcgctct atgcgggcct gaaccgcatc cacgacggca
      301 ccggcgttct ggagctggag caggtgcgca agatcgccac cgccggcgtc taccagacgc
      361 ccgtgctggg gaacaacgtg gcagtcgtcc tctcggtggg ttccgagaat atggacctcg
      421 cggtcgcgca ggatctgatc acggcgttcc tggagacgag cgccatgaac caccgcttcc
      481 gcgtcttcga ggtcctggcg ctgcgcatca agcggccggg agcaatctgc accatcgagc
      541 gcgcggagta gtcaggccgc ctaaagcgat cccttctcgg gcgcgcttag actgaccggg
      601 cggtcgtgcg aggcgtctca cgaccgccct ccccaacttc ctgccccagt ggccagtggc
      661 tcaatccggg cgaacgctct ctcctccgca gggacggatt gtggcccgct gccgcagtgc
      721 ggcctcttgc agataacgcc gcaccccccc gctgagcagc atgcgcaggt acgagaccgt
      781 gccgttcatc gcgcattcgg tgcacgagaa ccaatcgacc ggtgacgttc ggaaacgcgc
      841 agggcgcccc tttggaccgc gccggccaat ctcaccggtg tcttccttga cgatccacgg
      901 ctcgtcggcc gcatggatga tcacccggcc cttatgacac cacggacacg gcgtctctgt
      961 gatctgcatt gcccgctcct ccctccacga ggcccccgac agacaggacg cggcgcagcg
     1021 gctatccatt cccaaggtag ccgcccgcgt cacaagccat ctcccgcgcg tgcgagagct
     1081 tgggatagga agatccatcc agccacccgc atcctccatg ccactccgga ctccccccca
     1141 cgctcccgac gctatcgccc cggtttacac gctggcagaa gggcgctggc gccctttcca
     1201 cactccaata cgccggagcg ttacgcctgc ctgataagaa ctcgagacct tgcttgccgg
     1261 cgtcgcgcgc atgcagcgcc cgcgcgcctg ttcatcgccc ggtcatggcc tataccctcc
     1321 gcttgccatt tccccttgcc gccgctataa tggcgatacc gctactatac ccgcccatgg
     1381 gtgcagaaca accttgtgaa gaggagaaca gcggatggaa cggcttcggt tcgggatcat
     1441 cggagtgggc gctcgcggca tctgcaacgc gcgcgctgtt gccgcgcggc cggatataga
     1501 gctggttggc gtgtgcgata ttcgtcccga gcgcctcgag gagtgcgccc ggcaggggat
     1561 caccgcccct gggttcaccc actaccccga
//
LOCUS       DAOSCV010001026         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52385_length_1589_cov_2.504563, whole genome shotgun sequence.
ACCESSION   DAOSCV010001026 DAOSCV010000000
VERSION     DAOSCV010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52385_length_1589_cov_2.504563"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PLY56_18720"
     CDS             <1..1482
                     /locus_tag="PLY56_18720"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ23565.1"
                     /translation="GEPNLYTVTCELLHRGEPVDQRTDRIGLRTVRLQRTEVTGEAEG
                     EFRFEINGTPILVKGANWVPADAFHSRDAERYEACLSLFDDLGCNMVRCWGGGVYEDH
                     AFFDFCDAHGMMVWQDFAFACARYPQNPEFLEKVRREAEAVVRKLRNHPSLVVWCGDN
                     ECDDAWLWGGDDPGRNLITREVLPRVVHRCDPGRPFVPSSPYHAPEQVRRGESRLLPE
                     QHLWGPRDYFKSRFYTETTAHFISEIGYHGCPNVSSMRRFLDEEHLWPWQENSQWITH
                     CTDSTPGGGPYKYRVRLMADQIRELFGFEPETLEEFALASQISQAEAKKFFVEMVRLR
                     KWRRTGVLWWNVIDCWPQFSDAVVDYYFGKKLAYYYLRRVQEPVCLMIDEPENWHVRV
                     VAGNDSRSPAEGPFRVWDADTGETLLEGEFHTQANANQEIGRIRVSHGEHRLFLIEWS
                     VDGRKRGNHYLLGKPPIPFERYREWLRAIAALPAGFNPDEIAR"
BASE COUNT          263 a          491 c          554 g          281 t
ORIGIN      
        1 ggcgagccga atctgtacac ggtaacctgc gagttgctcc atcggggcga gccggtcgac
       61 cagcgcaccg accgaatcgg cctgcgcacg gtgcgcctcc agcgcacgga ggtcacgggc
      121 gaggccgagg gcgagtttcg gttcgagatc aacggcacgc cgattctggt gaagggcgcc
      181 aactgggtgc cggccgatgc ctttcactcg cgcgatgccg aacgctacga ggcgtgcctg
      241 tcgctcttcg atgacctcgg gtgcaacatg gtgcgttgct ggggcggggg cgtctacgag
      301 gaccacgcct ttttcgattt ctgcgacgcg cacggcatga tggtatggca ggatttcgct
      361 ttcgcctgcg cgcgctatcc gcagaacccg gagttcctgg agaaggtccg tcgcgaggcg
      421 gaggcggtgg tgcgcaagct gcggaatcac ccgtcgcttg tcgtgtggtg tggcgataac
      481 gagtgcgacg acgcctggct gtggggtgga gacgacccgg gccgcaacct gatcacgcgc
      541 gaggtgctgc cgcgggttgt tcaccggtgt gatcccggac ggccttttgt gccaagctcg
      601 ccctaccacg ccccggagca ggtgcgccgt ggcgaatcgc gcctgcttcc cgagcagcac
      661 ctctggggcc cgcgcgacta cttcaagagc cggttctaca cggagaccac ggcgcacttc
      721 atcagcgaga tcggctacca cggctgcccc aacgtctcct cgatgcgccg cttcctggat
      781 gaagagcacc tctggccgtg gcaggagaac tcccagtgga tcacccactg caccgactcc
      841 acgccaggcg gcgggcccta caagtaccgc gtgcgcctca tggcagacca gattcgcgag
      901 ctgttcgggt tcgagccgga gacgctggag gagttcgcac tcgcgtctca gatctcccag
      961 gcggaggcga agaagttctt cgtggagatg gtccgcctgc gcaagtggcg gcgcacaggg
     1021 gtcctctggt ggaacgtcat cgactgctgg ccgcagtttt ctgacgccgt cgtggactac
     1081 tacttcggga agaagctggc ctactactac ctccgccggg tgcaggaacc ggtgtgcctc
     1141 atgatcgatg agccggagaa ctggcacgtg cgcgtggtgg cgggaaacga ctcgcgctcg
     1201 ccagccgagg ggccgttccg cgtctgggat gccgatacgg gcgagacgct gctggaaggc
     1261 gagtttcaca cccaggcaaa tgccaatcaa gagatcgggc ggatccgggt ttcccacggc
     1321 gagcaccggc ttttcctgat cgagtggagc gtggacgggc gcaagcgggg gaaccactat
     1381 ctgctgggca agccgcccat ccccttcgag cgttaccgcg aatggttgcg ggcgattgcc
     1441 gccctcccgg ctggcttcaa cccggacgag atcgcgcgct aggcgaaggc ggccgggtat
     1501 tcctcctgag ggcgggcggc tgtggatgag cacgggaggt ggccgatgta cgagcgtatc
     1561 ctggtgccgc tggatggttc ggacgtcgc
//
LOCUS       DAOSCV010001027         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52529_length_1586_cov_4.005879, whole genome shotgun sequence.
ACCESSION   DAOSCV010001027 DAOSCV010000000
VERSION     DAOSCV010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52529_length_1586_cov_4.005879"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLY56_18725"
     CDS             complement(<1..337)
                     /locus_tag="PLY56_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23566.1"
                     /translation="MRKAIAVVTVALLGAAALYAGQAMREDSRPAAQRWQAVTGTVQA
                     APEGRSFVILSQRDGRVTVNARNATIRQLGRVVNFDAVKPGQVVTAFGTRTAGQITAE
                     EVCICPTTPE"
     gene            complement(684..1022)
                     /locus_tag="PLY56_18730"
     CDS             complement(684..1022)
                     /locus_tag="PLY56_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23567.1"
                     /translation="MPSYEELKNELCGFLVTQHNAMIHAFGAADKEDKELGPVRDFRL
                     TFRVAERADFDRVHASLRGYLTENYPSAFLTAYSAAEKDDKEGGRVREFRITIRVPLL
                     AGEEAPAEGA"
     gene            1199..>1586
                     /locus_tag="PLY56_18735"
     CDS             1199..>1586
                     /locus_tag="PLY56_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ23568.1"
                     /translation="MQLAGKVALVTGAGSGIGEASAVLFAREGARVGALDRTHEKLQA
                     VVGRIEQSGGEAMALVADVSHPGEMESAIRELTDRWGRLDIVFANAGINGVWAPLDAL
                     SPEEWNRTLSINLTGTFLTVKYAVPYL"
BASE COUNT          261 a          485 c          564 g          276 t
ORIGIN      
        1 gctcgggggt ggtggggcag atgcacacct cctcggcagt gatctgtccc gccgtgcgcg
       61 tcccgaaagc tgtgactacc tgaccgggct tcaccgcgtc gaagttcacc acgcggccaa
      121 gctggcggat cgtggcgttg cgggcgttga cggtgacccg gccatcgcgc tgcgagagga
      181 tcacgaaaga gcgtccctcg ggcgctgcct gcacggtgcc ggtcaccgct tgccagcgtt
      241 gggccgcagg gcgagagtcc tccctcatcg cctgtccggc atagagcgcc gccgcaccaa
      301 gaagagcaac cgttacaacc gcaatcgctt tcctcatgcc ccctcctccc aatcatttac
      361 aggcacatgg gaagggcccg ccgatgccca atacgccagg cctggcaggg tggcattgcc
      421 tgcgccctgg ccactgggcg tgcaacgaga gagccacggt aggcgggcct agcccgccct
      481 gggtgcaggg accgtgccaa agaccaaacg atggtcacgg ggatctgggg aaacggggag
      541 gatctggatg aggaaggggg cgccgttcgt ctgccgcctc atcggagccc gcgggtagct
      601 gagagacgcg acaacgggtc cccggggcct cgccggtgtg gtggggagcc gttgtcgcgg
      661 gacaatagct gtgctggcgt gggctacgca ccctccgcgg gcgcttcctc gccggcgagg
      721 agcggtactc gaatggtgat ccgaaactcg cggacccggc ctccctcctt gtcatccttc
      781 tccgcggcgc tgtaggccgt gaggaaggcc gagggatagt tctccgtcag gtagccgcgc
      841 agagacgcgt gaacacggtc aaaatcagcg cgctcggcga cgcggaaggt cagccggaaa
      901 tcgcgcactg gccccagctc cttgtcctcc ttatcggcag cgccgaaggc gtggatcatc
      961 gcgttgtgct gggtgaccag gaagccgcac agctcgttct tgagctcctc gtaggatggc
     1021 atttggtgat ggtctttcct ctcatcgatg gctgtcaggg gacatgcttg tttcattctt
     1081 tcgcggttgc ggcgcccggt tcctatgcga ggtggcggag ggcaggcggt ttgaaagtgg
     1141 gtcaaccggc ggtataactc cctatcgagc gcgggtggcg gcgatggagg gagagaggat
     1201 gcagctagct ggcaaggtgg ccctcgtcac gggggcgggc tctggaattg gcgaggcctc
     1261 ggcggttctc ttcgcgcgcg agggcgcgcg cgttggggcg ctcgaccgga cacacgagaa
     1321 gctgcaggcg gtggtgggcc ggatcgagca aagtggtggg gaagccatgg cgctcgtggc
     1381 agacgtctct catccgggcg agatggagag cgccatccgg gagctcacgg atcgctgggg
     1441 ccggctcgat atcgtcttcg ccaacgcggg gatcaacggc gtgtgggcgc ccctggatgc
     1501 gctgtcgccc gaggagtgga accgcacgct ctccatcaac ctgaccggga ccttcctcac
     1561 ggtgaaatac gccgtgccct acctga
//
LOCUS       DAOSCV010001028         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52547_length_1586_cov_2.697583, whole genome shotgun sequence.
ACCESSION   DAOSCV010001028 DAOSCV010000000
VERSION     DAOSCV010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52547_length_1586_cov_2.697583"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PLY56_18740"
     CDS             complement(<1..1049)
                     /locus_tag="PLY56_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23569.1"
                     /translation="MREGQPIGLTEEIMAKTGVRLEGGAEGAPERITLENDRLTATLH
                     RVGETYHLASLKAGGEYELLAEPSPLLFCYDGKEHLAVPFPGVAVEEETPEEVRLRLA
                     MNREGLAIELTLSLKTGRPFLEVEATCVSARDWQGLLYFSCLPAPGFIPTAYPWVAGK
                     ILQAEGFAYANCMGVPLLAGDPRGHDGHVLALGFPLSVNYRDLFFHTDTETRRITAGI
                     GEGISRPAATPAEEKAEDAPATPPKRGLLSWIRREREAEPPPPLPPLSLSIPYRAGVA
                     YRLPLHVWALTGQYRNLAGEWMKSTGFVFDNPEIRSVPESLAMTLAFLKDGAPFIDGK
                     GYPMRCARYTRAPGQG"
     gene            complement(1398..>1586)
                     /locus_tag="PLY56_18745"
     CDS             complement(1398..>1586)
                     /locus_tag="PLY56_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23570.1"
                     /translation="ADAAALLHPEVRDALMASSFESVVIRQGEVTVRQIGPVTDPEKL
                     RQSIQLAVDIAEWLETLG"
BASE COUNT          277 a          525 c          513 g          271 t
ORIGIN      
        1 ccctggccag gagcgcgagt gtagcgcgcg cagcgcatcg ggtagccttt gccatcgatg
       61 aagggcgccc cgtccttcag aaacgccagc gtcattgcca gcgattcagg caccgagcga
      121 atttcgggat tgtcgaagac gaagcccgtg ctcttcatcc actcgcccgc cagattccgg
      181 tattgccctg tcaacgccca gacgtgcagg ggcagccggt acgccactcc agcccggtag
      241 gggatggaaa gcgagagggg cggcagcggg ggaggcggct cggcttcgcg ctcgcgccgg
      301 atccatgaga ggagcccacg cttgggcggc gtcgcagggg catcttctgc tttctcctcg
      361 gctggtgttg ctgccggccg gctgatcccc tcgccaatgc ccgccgtaat gcgccgcgtc
      421 tccgtatccg tatggaagaa gaggtctcgg tagttgacgg aaagcggaaa cccgagggcg
      481 agcacatggc cgtcgtgccc gcgcgggtca ccagccaata gaggaacccc catgcagttg
      541 gcgtaggcaa agccctccgc ctggagaatc tttcccgcga cccacggata ggccgtcggg
      601 atgaaaccgg gggccggcag gcaggagaag tagagaagac cctgccagtc tctggcagag
      661 acgcacgttg cctccacctc cagaaaaggc cgaccagtct tgagcgagag cgtgagttcg
      721 atggccaggc cctcgcggtt catcgccagg cgcaggcgca cctcctccgg ggtttcctct
      781 tccaccgcca ctccggggaa gggcacggcc aggtgctcct tgccatcata gcagaacaga
      841 aggggcgatg gctctgccag cagctcatac tcgcctcccg ccttcaagct ggccaggtga
      901 taggtctcac ccacccgatg cagagtcgcc gtgagccggt cgttttcgag ggtgattctc
      961 tccggcgcac cctctgcgcc gccctccaat cgcacgcctg tcttcgccat aatctcctca
     1021 gtcaggccga tgggctggcc ctcccgcatc tcctattcca cggtctgctg ccggctcctg
     1081 cccctccaaa gccacgatgg gatcggttgc cccccgcgcg gggcgcggga atcaagagca
     1141 gccctccggg gtgtggaagc aggaagccaa tcggggatcg aattgggggc acttctcctc
     1201 acatccccag gtgccgctgg gtgaggggcg tggaccagca gagatcacct ggcaggggct
     1261 gctcgccacg aaggacgggg tccattccgg gggaagccgg gtgccgctct tccacgatct
     1321 ccccccgcat cctccgtggc gaagctttca gaagggcgtt gagtggtagc cgtgccggca
     1381 gaacaggcga tccagtgcta tccgagcgtt tccagccact ccgcgatgtc gacggccagc
     1441 tggatgctct ggcgcagctt ctcgggatct gttacggggc ctatctggcg aacggtgacc
     1501 tccccctggc ggatgaccac gctctcgaag gaagaggcca tcaatgcgtc gcgcacctct
     1561 gggtgaagca gcgcggcggc atcggc
//
LOCUS       DAOSCV010001029         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52674_length_1583_cov_3.645942, whole genome shotgun sequence.
ACCESSION   DAOSCV010001029 DAOSCV010000000
VERSION     DAOSCV010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52674_length_1583_cov_3.645942"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PLY56_18750"
     CDS             <1..254
                     /locus_tag="PLY56_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23571.1"
                     /translation="PSDLRFPELDPQVRAELDRRYPRVFSSGLANFSVEYDPVARIVT
                     LHWQSGMRNARLSEGALPRWSGWCVKVEERGRVVTIRER"
     gene            complement(276..1421)
                     /locus_tag="PLY56_18755"
     CDS             complement(276..1421)
                     /locus_tag="PLY56_18755"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ23572.1"
                     /translation="MLPKERVAAVFEHRPTDKVPIYQGGLSSRSASIILGREAYVGGG
                     IQQYREARALWEGPDAHQEFLERTRQDTFDLVRTLELDLVRPSYWRMPEKPTRRIDEN
                     TFFYGQEDGVWRVMRFDPAYELYQEIDHSPRPELTLEELEHCVAAAEKAAAEAPPPKP
                     ENYPDLLAAIAEFGETHAIPGSGIGICVPREPVWLEAIALRPDLVGRYLMAQATRAAR
                     VPAVMAQMGLRYLMGGGDFASKNGPFYSPRSFHQIMLPALQRVSEACHAAGTYHLFAS
                     DGDLWPVADDLFGASGVDSFYEIDRRAGMDLARLRDTFPHLTLLGGIASETLHMGTKE
                     EVIAEVRSALAVAKEKGGIIVGCSNLIVAPTPAENIWAMMETLHAER"
BASE COUNT          270 a          539 c          508 g          266 t
ORIGIN      
        1 ccccgtccga tctccgcttt ccggagctgg acccgcaggt tcgggcagag ctcgaccgcc
       61 gctacccgcg cgtcttctcc tccggattgg ccaacttctc ggtggaatat gaccccgtgg
      121 cacgcatcgt caccctccac tggcagagcg gcatgcgtaa cgcccgcctc agcgaggggg
      181 cgctcccccg ctggagcggc tggtgcgtga aggtggagga acgcgggcga gtggtcacga
      241 tccgcgagcg atgagccggg aaagggctcg ccaccttacc gctccgcgtg gagggtctcc
      301 atcatcgccc agatattctc cgccggcgtt ggtgcgacga tcaggttcga gcagccgacg
      361 atgatgcccc ctttctcctt cgccacggcg agggcgctgc gcacctcggc gatcacctcc
      421 tccttcgtgc ccatgtgaag cgtctccgag gcgatgccac ccaggagcgt caggtggggg
      481 aaggtgtcgc ggagccgggc cagatccatg ccggcgcgcc gatcgatctc atagaagcta
      541 tcgacgccgg atgcgccaaa gagatcatcg gccacgggcc agagatcccc gtccgaggca
      601 aacaagtgat aggtgccggc ggcgtgacag gcttccgaga cacgctgcag cgccggaagc
      661 atgatctggt ggaaggagcg cggggagtag aacggcccgt ttttcgatgc gaagtcaccc
      721 ccgcccatga gatagcgcag gcccatctgc gccatcaccg cgggcacccg cgcggcacgc
      781 gtcgcctggg ccataaggta gcggcctacc aggtcggggc gaagggcaat ggcctccagc
      841 cagaccggct cgcgcggtac gcagatgccg atgccacttc ctggaatggc atgcgtctcg
      901 ccaaactcgg cgatcgccgc gagaagatcg ggatagttct ccggcttcgg cggaggcgct
      961 tcggcggctg ccttctcggc tgcggcgacg cagtgctcca gctcctcgag agtcagctcc
     1021 gggcgcgggc tgtggtcgat ctcctggtag agctcgtagg cgggatcaaa gcgcatcacc
     1081 cgccagacac catcctcctg gccatagaag aaggtgttct cgtcaatgcg ccgcgtcggc
     1141 ttctccggca tccgccagta gctggggcga accaggtcga gttccagcgt gcgcaccaga
     1201 tcaaacgtat cctggcgcgt tcgctccagg aattcctggt gggcatccgg cccctcccac
     1261 agcgctcgcg cctcgcgata ctgctggatc ccaccgccca cgtaggcttc acgccccagg
     1321 atgatggacg cagagcgcga tgacaaccct ccctggtaga tcggcacctt gtcggtcgga
     1381 cgatgctcga agacggcggc gactcgctcc tttggaagca tgcgatggac tcctcacccg
     1441 ttgccggcgc ggggaaaaca cgcgttgccc cgcgggctat ccaccgtttc gctgccgacc
     1501 aggcctgccc ctgccccggg ccacgagtca gaaaggatat acgtctggcc gatcctcctt
     1561 ccggaagttc cacggctcgt tgg
//
LOCUS       DAOSCV010001030         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52767_length_1581_cov_3.630406, whole genome shotgun sequence.
ACCESSION   DAOSCV010001030 DAOSCV010000000
VERSION     DAOSCV010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52767_length_1581_cov_3.630406"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLY56_18760"
     CDS             complement(<1..622)
                     /locus_tag="PLY56_18760"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ23573.1"
                     /translation="MREITTDAIAGAVARLCIEACVDLPADVEAAIRAGLEREESSLG
                     REVLSLALENARLARERRLPICQDTGFVSVLVELGQEACVVGGDLNEAIHEGVRRGYA
                     EGRLRCSVLDHPLRRKNTGDNTPAAIHVDIVPGDRLRIRVMPKGGGCENMSALRILKP
                     SEGEEAVKQFVVETVFNAGPNACPPLVVGVGIGGTFDLCPHLAKRAL"
     gene            complement(687..1499)
                     /locus_tag="PLY56_18765"
     CDS             complement(687..1499)
                     /locus_tag="PLY56_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ23574.1"
                     /translation="MGVGKRLRVRSLASGSNGNCVVVQSGEDALLIDAGISRRAVLAG
                     LAATGIPEHAVRAILLTHEHSDHTRGLLPVARRLGAPVIANRATLAAAGACDLEHCVL
                     ETGATLELGALRLCSFGVSHDSVEPVGYVVEDGAHRVVCCTDTGVPGDLSERLKEADL
                     AILEANHDIDRLLAGPYPERLKRRILGDAGHLSNAAAAELASALLLHRPGAQVWLAHL
                     SAINNTPSLALATVRRQVAMAGGDPERVTVAPRGRPGPIWDAAETWRQLSLF"
BASE COUNT          269 a          590 c          485 g          237 t
ORIGIN      
        1 aaagcgcccg cttggccagg tgcgggcaca ggtcgaacgt gcccccaatt ccaaccccca
       61 cgacaagagg cgggcaggcg tttggcccgg cgttgaagac cgtttcgacc acgaactgct
      121 tcacggcttc ctcgccctcg gacggcttca ggatgcgcag cgcgctcata ttctcgcagc
      181 cgccgccctt cggcattacc cggattcgca gtcggtcgcc cggcacgata tccacgtgga
      241 tcgccgcggg ggtgttgtcg cccgtgttct tcctgcgcag cgggtggtcg agcaccgaac
      301 accggagccg gccctcggca tagccacggc gcacgccctc gtgaatcgcc tcgttcagat
      361 cgcccccgac gacgcacgcc tcctggccca gctccacgag gacggagaca aaaccggtat
      421 cctggcagat cggcagcctg cgctcgcggg ccagccgggc gttctccagg gccaacgaaa
      481 ggacctcgcg gcccagggac gactcctcgc gctccaggcc cgcccgaatc gctgcttcca
      541 cgtcggcggg cagatcgacg cacgcctcga tacaaagccg ggccacggcg ccggcaatcg
      601 catccgttgt gatctcgcgc aacccaacct cctcgggata cctcttcggg tccgcactgc
      661 ccgtttcctg tccgcgcgcc acccatctaa aagagcgaca gctgccgcca ggtctcggcg
      721 gcatcccaga tggggccggg ccggccgcgc ggggctacag tgacgcgctc ggggtcacca
      781 cctgccatag ccacctgccg gcgtaccgtt gccagcgcca gcgacggggt gttgttgatc
      841 gcgctcaggt gcgccagcca gacctgcgcg cccggccggt gcaacagcag cgcactggca
      901 agctccgccg cagccgcatt cgagaggtgg cctgcatccc ccaggatgcg ccgcttcagc
      961 cgctcgggat atggcccggc cagcaagcgg tcgatatcgt ggttcgcctc caggatggcg
     1021 aggtcggcct ccttcaggcg ctcgctcaga tcacctggca cccccgtgtc ggtgcagcag
     1081 accacccggt gtgccccgtc ttccaccaca tagcctacgg gctccaccga gtcgtgcgaa
     1141 acgccaaaag agcacaggcg gagagcgcca agctcgagcg tcgcgccggt ctccagcacg
     1201 caatgctcca ggtcacacgc tccggcggca gccagggtcg cgcggttggc aatcaccggc
     1261 gcgcccaggc ggcgcgccac gggcaacagc cctcgggtgt gatccgagtg ctcgtgtgtc
     1321 aggaggatgg cgcggacggc gtgctccgga attccggttg ccgccaggcc cgccagaacg
     1381 gcgcgcctgc tgatccccgc atcgatcagc aaagcgtcct cgcccgactg gacgacgaca
     1441 cagttgccgt tactcccact ggccagcgag cgaacgcgca gccttttccc cacacccacg
     1501 acccgctctt cctctccaat agccctattg tacgcagaga tggtcaaatg atcaagcggt
     1561 agccggcccg caaaaccacg g
//
LOCUS       DAOSCV010001031         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52897_length_1578_cov_3.381484, whole genome shotgun sequence.
ACCESSION   DAOSCV010001031 DAOSCV010000000
VERSION     DAOSCV010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52897_length_1578_cov_3.381484"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLY56_18770"
     CDS             complement(<1..528)
                     /locus_tag="PLY56_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ23575.1"
                     /translation="MRSQQPLRRQDWSGRSPQEILDSHHPGKTDPLRVLDVLLALHNG
                     RHTIFEKSVSHKTRQERARFLRRFFRDLKLKAGFHKPPDPRNLGQKHIHAMVQVWQRE
                     QLAPATIQTYLSFLRGLASWMGKHGFVRKPERYGLSLEEYERHEYAQRDKSWRGNGVD
                     VEAALAEVARFDPRIA"
BASE COUNT          256 a          479 c          575 g          268 t
ORIGIN      
        1 cgcgatgcgc ggatcgaaac gggcaacctc ggccaacgcc gcctccacgt cgacgccgtt
       61 gccgcgccag ctcttgtcgc gctgtgcgta ctcgtgacgt tcgtattcct ccaggctcag
      121 cccgtagcgc tcgggcttgc gcacgaagcc gtgcttgccc atccaggagg ccaacccccg
      181 caggaagctc aggtaggtct ggatggtggc cggcgccagc tgttcccgct gccagacctg
      241 caccatcgca tggatgtgct tctgaccgag gttgcgcggg tcgggcggct tgtggaagcc
      301 ggccttgagc ttcaggtcgc ggaagaaccg acgcaggaag cgcgcgcgct cctgccgggt
      361 cttgtgcgag acgctcttct cgaagatggt gtgccggccg ttgtgcagcg cgagcagcac
      421 gtccagcacc ctgagcgggt cggtcttgcc ggggtggtgg ctgtcgagaa tctcctgcgg
      481 cgaccggcca ctccagtcct ggcggcgcaa cggctgctgc gagcggatgc gtcttgcggg
      541 tgtcggtccg tggaccaggt gggttgatgc tgggaactct gatggcgtga acatgtaggg
      601 cttcctcaag gtttgaatgc gcctcccgtc cggcgcactc gtagatgaac aggcatcgtc
      661 gttgctggca cgccgcggat gccatcgcgg cgtgccggtg gtggtctgtg atcacgtcgc
      721 cgcgcccgac cagaggaggc gcgacgacga cccaagggat ttgcggcagg gcagccaaac
      781 gccgctcggt cggtcttaaa aatgaacacg tgatccggct ccgtccgcgc atttctgcga
      841 ccacggagat gggtgacgct gatcgcctct ggggcagatc ggcatcgagc gaggaggtct
      901 ggcctcccgc cgactggtgg atcaatcctg gattcgtagc gtcatccgct ggcatgggtc
      961 cacccctgac cgaaagtcca gggcggcgcg agcgtttgag cgagagcgag cagggagccc
     1021 atagggcaag cacgactgcg cgcggcgttg aacaacgcat gctcgggtga tgacgggatc
     1081 gggaacgcgg catccagtgc cggcccggtg acgacggcca cgacgcgctt caggagcgcc
     1141 cagccggagg ctggctgggt cgatggcgca tgcactacga gaagggaacc tcggcgccat
     1201 cacctccggg gtgatggccg caccggctcg gggccggggc ttcaagggca ggtgacgact
     1261 gcgacgcctc tttgctgcac agggcagggg gctgagggtg ccctcgggag tgggtagttg
     1321 acgatggatg gctggtcgcc gtcaatgcgc aggcccgcaa gcgacgcgat gacgcggggc
     1381 ttgacggtgg cggacgacgt gctagcgagc gggcggtgag ggccgttttg tgtcgcaaac
     1441 ggctctggaa agtaaatcca cgagggagtt gacaagcact tcgcccgcct ctggcctcgg
     1501 ctggggcgct ggaggccggc attcctcaga gggctccgcc acatcggcgg cgagccgagc
     1561 agggttgaaa accctcgg
//
LOCUS       DAOSCV010001032         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52904_length_1578_cov_2.747209, whole genome shotgun sequence.
ACCESSION   DAOSCV010001032 DAOSCV010000000
VERSION     DAOSCV010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52904_length_1578_cov_2.747209"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PLY56_18775"
     CDS             <1..393
                     /locus_tag="PLY56_18775"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23576.1"
                     /translation="ETIPVGVALLIRPRIHPDQSVTLKVHPVVSTVSEIIDGLPQTAS
                     READTTVRLARGEQLVIGGLDRDEIVRTVRRLPILSDIPLVGELFKTRSTRTATTELL
                     IFVAAYPVEPEAAPPVSEFPRPILELEP"
     gene            603..>1578
                     /locus_tag="PLY56_18780"
     CDS             603..>1578
                     /locus_tag="PLY56_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23577.1"
                     /translation="MASGETALAPHADPFVQLPDPPAVAATSRPPSLPPLATASTGGS
                     QLPTLPAVSWPGGPGILPPIRPIPGGGAAGPAGTTWPRQAHTPRPLPPKPELIGTLLG
                     ARPSAVFRSGGKLTVVSISETIGGWRLLTVEHGGVVIRSGNETARILVGGEPSSGIAT
                     SVSSPPVAPALSPTAPLAPPEQPPAAQPSPAQPSPQPAPENPPLKVPRSQPVAHATPT
                     ALGASTPAAEASTENVAIAEKVGAASPPAVTLEPVHSVTLAVEGSLTAQAAAMPSPPA
                     TAQPKRTPAITLASAPNEGVATAEAHAEHAVVGQQPAHLLAAEPSATPL"
BASE COUNT          275 a          586 c          514 g          203 t
ORIGIN      
        1 gagacgatcc cggtgggcgt ggcgctgctg atccggccgc gcatccaccc ggaccaatcg
       61 gtcacgctga aagtacaccc ggtcgtgagc accgtctcgg agataatcga cggcctgccg
      121 caaacggcga gccgcgaggc ggatacgacc gtgagactgg cgcgcggtga gcagctcgtc
      181 attgggggat tggaccgcga tgagatcgtc aggacggtgc ggagactgcc catcctgagc
      241 gacattccgt tggtaggcga gctattcaag acgcgatcca cgcggacagc gacaacggag
      301 ctcctcatct tcgtagcggc ctacccggtg gaacccgagg ctgctccgcc tgtgagcgag
      361 ttcccccgac ccatactgga gctggagcca tgagcaacac ccaaaagagc agtcctgcca
      421 cgatagcagc actgatcgcg ggctgctgtc tggcggtcgg gttcgccgca tggaacatca
      481 cgcgagccat gcgcccggcg gctccgcccg ctgacacggc ctcgacggcc aaggaggaca
      541 cggcgccggt tccattggag ttggcagggc cacctccact caccccggcg caggagcggc
      601 gcatggccag cggtgagacg gcactcgcgc ctcacgcgga cccgttcgtg cagcttccgg
      661 atccccccgc ggttgcagca acgagccgtc cacccagcct tccacccctg gcgacggcat
      721 ccacgggggg cagccagctg ccaacgctcc cggcggtctc ctggccaggc gggccaggga
      781 tactcccgcc gatacgtccc atcccggggg gaggggccgc agggccggct ggaaccacct
      841 ggccacgcca ggcgcatacc ccccgcccgc tgccgccgaa accggagctg atcggcacgc
      901 tgctgggcgc tcgacccagc gccgtcttcc gtagcggcgg caagctcacc gtggtctcca
      961 tcagcgagac catcgggggc tggcgtcttc tcaccgtcga gcacgggggc gtcgtgatcc
     1021 gctctgggaa cgagacagct cgcatcctgg tgggtggaga gccctccagc gggatcgcga
     1081 cctccgtatc tagcccaccg gtggcgcccg ccctctcacc aacggcgcct ctcgcgcccc
     1141 cggagcaacc accggccgcg cagccatcgc ctgcgcagcc atcgccacaa ccggcgcccg
     1201 agaacccgcc gctcaaggtg ccgcggtcgc agcccgtggc acacgcaaca cccaccgcgt
     1261 tgggtgccag cacgcccgcg gcggaggcga gcacggagaa tgtggcaatc gcagagaagg
     1321 taggggcggc ctcgccgccc gccgtcacac tcgagccagt gcattctgtc actctcgcgg
     1381 ttgaggggag cctcaccgcg caggcagcgg cgatgccctc tcccccagca acggcgcaac
     1441 cgaaacgcac accggccatc acactcgcct cagcaccaaa cgagggggtg gcaaccgccg
     1501 aagcgcacgc ggagcacgcg gtcgtggggc agcagccagc gcatctgctg gccgccgagc
     1561 cctctgcgac cccgctca
//
LOCUS       DAOSCV010001033         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_52946_length_1577_cov_3.016426, whole genome shotgun sequence.
ACCESSION   DAOSCV010001033 DAOSCV010000000
VERSION     DAOSCV010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52946_length_1577_cov_3.016426"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLY56_18785"
     CDS             <1..1414
                     /locus_tag="PLY56_18785"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOJ23578.1"
                     /translation="IEEYHQPGSRGITYEVTVAGQPRMQLTYEHNGRIIVINKGTRKS
                     EIEEAQQGFILCKACHRWLFGEKSVKNHTASNSDGRCPRGGTPDDIVRRIVLFTDSQH
                     DVVIVECPLPEGVPTNRAYDFYVTLLHALLQGVQISLDVDESEVAGFLARTPGSATGY
                     RVILYETQEGGTGVVQALVDTERFQGIVARAREILHDGEQGCERACYECLCSFYNQID
                     HAHLDRTLVLPFLKSLATAVVSPTNGDGPGNNRLQELLAKCQSDFERRVLQKIHDAGL
                     PLPDEAQKLVREKDGRPIASADFFYEPNIVIFVDGSPHYKDYVAAADNAKRQRLKALG
                     YRIVVFTGDEAQIEGQVATLAQRIGVSPKAHTAPTPVPAGEDKLSGVLRYCAPECHEI
                     VRACAREGLPLPEVGYEPEDASGAVCGQVELAWPEQKVAVALPDQADGKAALEAQGWA
                     VLSAAEAAAEPETLLARLTG"
BASE COUNT          318 a          479 c          521 g          259 t
ORIGIN      
        1 catcgaggag taccaccagc cgggcagtag aggcatcacc tacgaagtga cggttgctgg
       61 ccagccgcgg atgcagctca cctacgagca taatgggcgc atcattgtca tcaacaaagg
      121 cacgcgcaag agcgagatag aggaggcgca gcagggcttc atcctctgca aagcatgcca
      181 ccgctggctc ttcggcgaaa agagtgtgaa gaaccacacc gcgtccaatt cggatgggcg
      241 ttgcccgcgc ggcggcactc cagacgacat cgtgcgccgc atcgtgttgt ttaccgactc
      301 gcagcacgac gtggtgatcg tcgagtgccc tctccccgag ggagtgccta ccaaccgggc
      361 gtacgatttc tacgtcacgc tgctgcatgc gctgctccag ggcgtgcaaa tcagcctgga
      421 cgtggatgag agcgaggtcg cgggcttcct cgcacgcacg ccgggctccg ccaccgggta
      481 ccgcgtcatc ctctacgaga cccaggaggg cggcaccggc gtggtccagg cgctcgtgga
      541 taccgaaagg ttccaaggta tcgtagcgcg agcccgcgag atcctgcacg atggcgagca
      601 gggctgcgag cgggcgtgct acgaatgcct ctgcagtttc tataaccaga tcgaccacgc
      661 gcatctcgac cgcacgctcg tgctcccgtt cctcaaatcg ctcgcgacgg cggtggtctc
      721 gcccacgaat ggcgatgggc ctggaaacaa ccgcctgcag gaactcctgg cgaagtgcca
      781 gtcggatttc gagcgcaggg tgctgcaaaa gatccatgac gcgggcctgc ccctgcccga
      841 cgaggcgcag aagctggtgc gcgagaagga cggcagaccc atcgcctcgg cggacttttt
      901 ctacgagccg aacatcgtca ttttcgtcga tggctcgcct cactacaagg actacgtggc
      961 cgccgccgat aacgccaagc gccagcggct gaaagccctt ggctaccgga tagtggtctt
     1021 cacgggagat gaggcgcaga ttgagggcca ggtcgcgact ctcgcgcaga ggattggtgt
     1081 gtccccgaag gcgcacactg cgccgacccc ggtgccagcc ggggaggata agctctcggg
     1141 ggtgctgcga tactgcgcgc ccgagtgcca tgagattgtc cgggcatgcg cccgagaggg
     1201 cctgccactc ccggaagttg gctatgagcc ggaagatgcc agtggggcag tgtgcgggca
     1261 ggtggagctc gcctggccgg aacagaaggt ggccgtggca ctcccggatc aagccgacgg
     1321 gaaggcagca ctggaagcac agggctgggc cgtcttgagc gcggcggagg ctgccgccga
     1381 acccgaaacg ttgctggcac ggctgacggg atagaccgca atgtctgata tcggctttaa
     1441 gcccaaggtg gcgatctcga tggagttcct ggagcgtttc gggcagcttc cgccggaagt
     1501 gcgtcagaag gccaagcgct tcgtggagaa gttccgcgag aacccggtct ccaaagcaat
     1561 caactatgag aaaatcc
//
LOCUS       DAOSCV010001034         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53065_length_1574_cov_4.282423, whole genome shotgun sequence.
ACCESSION   DAOSCV010001034 DAOSCV010000000
VERSION     DAOSCV010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53065_length_1574_cov_4.282423"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..>1574
                     /locus_tag="PLY56_18790"
     CDS             13..>1574
                     /locus_tag="PLY56_18790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ23579.1"
                     /translation="MQHPKAPGQIIHFLSSYYPARNRAGEVEGVGVTVMDITKRHQME
                     EALRASDEATNLIIDTIPASIAVIDERGVITRVNKAWERFRQENGGDPAKTGVGASYL
                     EVCRRATGAWSEGASRCYEGLRAVLAGKCPGFDLEYPCPSGEVMRWFLLRLVPMPQGR
                     GALAAHIDITEVKRLQEELRQRTSELDATFRALPDLYFRVDAEGTYLDIRAGKPSDLY
                     LPVKELLGKRIRDVLPPDAVRQVEEAIEETLETGKTTRTEYPLVIAGEPQVFEMRLSP
                     LGNRQAVAVVRNITEQKRTNEALRASEENYRMLVERISDWIWEVDAEGVFTYSSPKVR
                     DLLGYDPAEVVGKTPFDFMPVEEGKRVRQQFRAVAARREAFSGQENHYYRKDGSIVIL
                     EASATPIFDESGRFRGYRGIDRDITAKKRMAEAERFLAKAMQALSGSLDATALLATLA
                     RLAVPILGDWCIAYALDETGNAHRTALEYANPARADLAKRLLAMPTPPIHQAASYLES
                     LRRGHPLVIPTL"
BASE COUNT          292 a          507 c          523 g          252 t
ORIGIN      
        1 ctggagctgc cgatgcagca ccccaaggct cccggacaga tcatccactt cttatcgagc
       61 tactatcccg ccaggaatcg cgccggcgag gtggagggcg tgggcgtgac cgtcatggat
      121 atcaccaaac gccaccagat ggaggaggcg ctccgcgcca gcgacgaggc gacaaacctc
      181 atcatcgaca ccatccccgc cagtatcgcc gtcatcgatg aacgcggcgt gatcacccgc
      241 gtgaacaagg cttgggagcg cttccgccag gagaatgggg gtgaccccgc taagaccggc
      301 gtgggcgcga gctacctgga ggtgtgccgg cgcgccaccg gggcctggtc ggagggcgcc
      361 agccgatgct acgagggcct gcgcgccgtc ctggccggca agtgtccggg attcgatctg
      421 gaatacccct gtcccagtgg cgaggtgatg cgctggttcc tactgcggct ggtgccaatg
      481 ccccagggcc gtggggcgct cgcggcgcat atcgacatca cggaggtgaa gcggcttcag
      541 gaggagctcc gccagcgcac ctccgagctg gacgccacct tccgcgcgct gccagacctc
      601 tatttccgcg tggatgccga gggcacttac ctggatatcc gcgccggaaa gccgtcggat
      661 ctctacctcc ccgtgaagga gctcctcggg aaacgcatcc gcgacgtcct cccgccggac
      721 gcggtgcgcc aggtggagga agcgattgag gagacgctag agaccgggaa gacgacgcgc
      781 accgagtatc cgctcgtgat cgcgggcgag ccccaggttt tcgagatgcg gctgtctccg
      841 cttgggaatc gccaggcggt tgccgttgtt cggaacatca cggagcagaa gcggaccaat
      901 gaggcgctgc gcgccagcga agagaattac cggatgctcg tcgagaggat cagcgattgg
      961 atctgggagg tggatgccga gggcgtcttc acctactcca gcccgaaggt gagggatctc
     1021 ctcggttatg acccggcgga ggtggtaggc aagaccccgt ttgacttcat gcccgtggaa
     1081 gagggaaagc gcgtgcgcca gcagttccgt gcggtcgcgg cgaggcgcga ggcgttttct
     1141 ggccaggaga accattacta tcgcaaggat ggaagcattg tcatcctgga agcaagcgcc
     1201 acccccatct tcgacgagtc cggccggttt cgcggctatc gtggcattga ccgcgacatc
     1261 accgcgaaga agcgcatggc ggaggccgag cgcttcctgg caaaggcgat gcaggccctc
     1321 agcgggtcgc tcgacgcgac ggcactgctc gccacgctcg cccggcttgc cgtgccgatc
     1381 ctgggcgatt ggtgcatcgc ctacgcgctc gacgagaccg gcaacgcgca tcgcaccgct
     1441 ctggagtatg ccaacccggc gagggccgac cttgcaaagc gcctcctggc gatgccgacg
     1501 ccgcccatcc accaggcggc atcctatctc gagagcctgc gtagaggcca tccgctagtc
     1561 atcccaacac tgca
//
LOCUS       DAOSCV010001035         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53080_length_1574_cov_2.819618, whole genome shotgun sequence.
ACCESSION   DAOSCV010001035 DAOSCV010000000
VERSION     DAOSCV010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53080_length_1574_cov_2.819618"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1039
                     /locus_tag="PLY56_18795"
     CDS             362..1039
                     /locus_tag="PLY56_18795"
                     /inference="COORDINATES: protein motif:HMM:NF019231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOJ23580.1"
                     /translation="MMPRRLNRGGDRGFTLIELLVVIAIISMLASMLMPVYSRARESA
                     RKIVCTSNLKQIATAISMYTADHDDCYPIAWAFWGPVNSPPLPMTPNLKTCVGPYVKN
                     DEVWWCPTWSGRYGINAWGNPNGSGFDFIVPSATTQEVIGAPASDTSPGSCWSDASLT
                     MPTDYPLLWCGSHWTNALNAHSGANDGAFRAGNDRGGTNIAYADGHVKWVPLDWNKWI
                     QIYQTRR"
     gene            1060..>1574
                     /locus_tag="PLY56_18800"
     CDS             1060..>1574
                     /locus_tag="PLY56_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23581.1"
                     /translation="MITGILLRLALCALFFAVARYALKPETFSTMLGDLAERFYKPRD
                     AFDKANAYLPRLVQLCYAFSALFFIFTWTFLYHHVRQLKVVTDPEGGLRPYTAPATPG
                     GANPYSGLAPGQPSAQPGAGQLYGGQTYGGQPQGQPAQPYGGQSQGQPAQPYGGQPQG
                     QPAQPYGGQPYG"
BASE COUNT          273 a          504 c          523 g          274 t
ORIGIN      
        1 taccatgccc gcgtatcgct tgcaagggga acggcggccc tgcccttggg acgcggtcga
       61 taccccggat gaaaggggtg gacacggatg gggagggtgg gtgtgcctgg ggttgtgcga
      121 acccctcggg ggtggggaaa gcgcgtttac ggcgggctct ctctcgcccc gagccctggg
      181 tgatgggggg gtgcccatcg agcgttcgta gaacaagaag gggaacgcgg gagtgcgtcg
      241 aatggggcaa agagcccggt gacgacccac cttccatcgc gcgtgccggg ccggagtggg
      301 gcttgacgcg gcgacgtgcc ggggtgccgt tgctgcaagg cgcactgctt cggaggatgc
      361 gatgatgccc aggcgactga accgcggggg agaccgcggc ttcaccctca ttgagctgct
      421 tgtggtgatt gccatcatta gcatgctcgc cagcatgcta atgcctgtct actcgcgcgc
      481 gagagagtcg gcgcgcaaga tcgtctgcac cagtaacctg aaacagatcg ctacggcgat
      541 cagcatgtac acggccgacc acgatgactg ctatccgatc gcctgggctt tctggggccc
      601 ggtgaactcg ccaccgcttc cgatgacgcc caacctcaag acgtgcgtcg gcccctacgt
      661 gaagaacgat gaggtgtggt ggtgccccac ctggtccggg cgctacggga tcaacgcctg
      721 gggcaacccc aatgggagtg ggttcgactt catcgtgcct tccgcgacca cacaggaggt
      781 gatcggcgcc cctgcctcgg acacgagtcc tggctcctgc tggtcagatg cgtctttgac
      841 gatgccgacc gactacccgc ttctctggtg cgggtcccac tggacgaacg ccctcaatgc
      901 ccactccgga gcgaacgatg gggcgtttcg agcagggaac gaccgaggcg gaacaaacat
      961 cgcctacgcc gatgggcacg tgaagtgggt gcccctcgac tggaataagt ggatccagat
     1021 ctaccagacc cggcgctgac cgggcgggga gagagagcga tgatcacggg aatcctcttg
     1081 cggctggcgc tgtgcgcgct cttcttcgcg gtggcccgct atgccttgaa gccggagacc
     1141 ttctcgacga tgctcgggga tctggcggag cgcttctaca agccgcgcga tgccttcgac
     1201 aaggcgaacg cctatctgcc ccgcctggtc cagctttgct atgcctttag cgcgctcttc
     1261 tttatcttca cctggacgtt cctctaccat cacgtgcgcc agctgaaggt agtgaccgac
     1321 ccggaggggg gcctgcgccc ctacaccgcg ccggccacgc cgggcggggc aaatccctac
     1381 tcgggcctgg cccctggcca accatccgcg cagcctggcg cggggcagct ctatggtggc
     1441 cagacctacg gtggccagcc tcagggccag ccggcacagc cctatggcgg ccaatctcaa
     1501 ggccagcccg cacagccgta tggtggtcag cctcaaggcc agccagctca accctatggc
     1561 ggccagccct acgg
//
LOCUS       DAOSCV010001036         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53176_length_1572_cov_3.044825, whole genome shotgun sequence.
ACCESSION   DAOSCV010001036 DAOSCV010000000
VERSION     DAOSCV010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53176_length_1572_cov_3.044825"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLY56_18805"
     CDS             <1..196
                     /locus_tag="PLY56_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23582.1"
                     /translation="RTERDALRSEADALRGKLGAVAAPTVERRVAEIEARLARLETMG
                     TGMGTEPTARAREPKQGRSE"
     gene            197..838
                     /locus_tag="PLY56_18810"
     CDS             197..838
                     /locus_tag="PLY56_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23583.1"
                     /translation="MSNLAVLPKEVKAQPELHPRSEGIKKGVEYIRRLRVDAERTWPM
                     FARALATVGLIRQTAAVAAAFWDEASESRPACHRHAAEGRWAILEPKLAEELRRGLPL
                     KLAAARVGVPYASVRNRSAADPEMGARLEIARLQGVAKIHGYVVERAATSDSAAKSLL
                     EWSGEPEYTPRLKIQEVDEGEIVRSKAWARLTSRLAGVLCPECRARAAEAVGE"
     gene            996..1355
                     /locus_tag="PLY56_18815"
     CDS             996..1355
                     /locus_tag="PLY56_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23584.1"
                     /translation="MLKTKRVALSVRVSEHVRDALYRKAEREGHRYALALAASLLERA
                     VEVPAAGTGRGDWLSDMGALARVFGGKVSVDGDRVTLTLESGSKEQALVWLQSRVMAN
                     HPPPDGVVFMVKIADGS"
BASE COUNT          262 a          440 c          606 g          264 t
ORIGIN      
        1 ccggaccgag cgagacgcgc tgaggagcga ggcggacgcg ctgaggggca agctgggggc
       61 agtggcggcg ccaaccgtcg agcggcgggt ggccgagatc gaggcgcgcc tggccaggct
      121 ggaaacgatg ggtacaggga tgggtacaga gcccaccgcg cgcgcgcgcg agcccaagca
      181 aggcaggagc gaataggtga gcaatctcgc ggtcttgccg aaagaagtta aggcgcaacc
      241 tgagcttcat ccgcgaagcg aagggatcaa aaagggcgtg gagtacatcc ggcggttgcg
      301 cgttgatgcc gagcgaacgt ggccgatgtt tgcgcgggcc cttgcgacgg tggggctcat
      361 ccggcaaacg gcggccgttg ctgcggcttt ttgggacgaa gcctcggaga gccggccggc
      421 gtgccatcgt cacgcggccg aagggcgctg ggccatcctc gagccgaagc ttgcggagga
      481 attgcggcgc ggtctaccgc tgaaactggc cgcggcgcgg gtgggcgtgc cctacgcgag
      541 cgtgcgaaac cggagcgctg ccgaccctga gatgggcgcg cgcttggaga tcgcgcggct
      601 ccagggcgta gcgaagatcc acggttacgt cgtggagcgg gctgcgacgt cggacagcgc
      661 ggcgaaatcg ctcctggagt ggtccggcga gccggagtac accccgcggc tcaagatcca
      721 ggaggtggac gagggcgaga tcgtgcgctc gaaggcgtgg gcgcggctca cgtcgcggct
      781 cgcgggggtg ctgtgcccgg agtgccgggc gcgggcggcg gaagcggtgg gggagtgatg
      841 gcgcacgctc cgagcacctg cgagcatgcg agagcgtcgc tttgacgaat gcccggagat
      901 gctctcgcat gctggcggcg tatcgtcgga gcatctcacg gcgtctcccg catgctcgct
      961 tgcggcatct cggagcatgc agtagaatct cccgcatgct gaaaaccaag agggtcgcgc
     1021 tatctgtcag agtctcggag catgtgcggg atgctctcta tcggaaggcc gaaagggagg
     1081 ggcaccgata tgcgctcgcc ttggcggcct cgctgctgga gcgtgcggtg gaagttccgg
     1141 cggcgggaac gggccgcgga gattggctgt ctgacatggg ggctttggct cgcgtgttcg
     1201 gcggtaaggt gtcagtcgat ggcgatcgcg tgactctcac gctggagtct ggatcgaaag
     1261 agcaagcgtt ggtgtggctt cagtcgcgcg tgatggccaa tcatcctccg ccggatggtg
     1321 tggtgttcat ggtcaagatc gcggacggct cgtgagccgc ttgatccacg gcccgtggat
     1381 gcgcgatatt cccgatcaag acgcggatct ttgcttgatt gggcacgtct gcggaccgca
     1441 gcgcaacgcg gatattgcca ggctccaacc gtcgttgcgg gcgatgaatc ttccgccccc
     1501 gacgctgtgc gttgaccctg ttttgctagc gaagcgagcg ggactgtgtt cgcgatgccg
     1561 gagggcgcct gt
//
LOCUS       DAOSCV010001037         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53207_length_1571_cov_4.298153, whole genome shotgun sequence.
ACCESSION   DAOSCV010001037 DAOSCV010000000
VERSION     DAOSCV010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53207_length_1571_cov_4.298153"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLY56_18820"
     CDS             <1..282
                     /locus_tag="PLY56_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOJ23585.1"
                     /translation="MALGPRASNLTNRGIVSWGPYAIVRHPAYIAKNLAWWLEKLPMM
                     STPLHALSLLGFNTLYFLRALTEERHLRADPDYIAYCQKVRYRFIPGVF"
     gene            complement(294..503)
                     /locus_tag="PLY56_18825"
     CDS             complement(294..503)
                     /locus_tag="PLY56_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23586.1"
                     /translation="MRRTQPRRVQEGRGGCQGAILGLILGVVAGALIYNATGASEVVA
                     IVLGVIGLFLGAQVGQRLARRRQRR"
     gene            complement(570..710)
                     /locus_tag="PLY56_18830"
     CDS             complement(570..710)
                     /locus_tag="PLY56_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23587.1"
                     /translation="MSGRSADALIVTLSRGSVNAPVIPRGGILPGVEIARLPACRQEG
                     GA"
     gene            816..>1571
                     /locus_tag="PLY56_18835"
     CDS             816..>1571
                     /locus_tag="PLY56_18835"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23588.1"
                     /translation="MIRLGIVDFDTSHVVQFTKRLNHIDIAEEQWVEGAKIVMGYQGT
                     SRITPQERIDEYTAQVKAMGVELVDRPEEMIGKVDGILVESQEGAVHLERARPFLEAG
                     IPCYVDKPFATSVADAKAIVALANKHNVPVFSSSSLRYALEIQKLQEEGVGKIFGVDA
                     YSPASLHEYNPGLFHYGIHGVETLYALMGPGCESVTCTFTEGGEVTTGLWKDGRIGVM
                     RGTRAGSHSYGFVAWTEKGVRQVTIDANYIYREL"
BASE COUNT          296 a          554 c          461 g          260 t
ORIGIN      
        1 atggcgctgg gcccgagggc aagcaacctg accaaccgag gaatcgtatc gtggggcccg
       61 tatgccatcg tccggcatcc ggcctacatc gcaaagaacc tggcgtggtg gctggaaaag
      121 ctgccgatga tgtccacgcc actccacgcg ctctcgcttc tgggcttcaa caccctctac
      181 ttcctgcgcg ccctcaccga agagcgtcac ctgcgcgccg acccggacta tatcgcctac
      241 tgccagaaag tgcgctaccg cttcatcccc ggagtgttct aggcacgctc gccctaccgc
      301 cgctggcgcc ggcgtgccag gcgctgcccg acctgcgccc ccaagaagag gccaatcacc
      361 cccaaaacaa tcgccactac ctcactcgcg ccggtcgcgt tatagatcag agcgcccgcg
      421 accaccccca aaatcaggcc cagaatcgcc ccctggcatc ctccccggcc ctcctgcact
      481 ctccgcggct gcgtcctccg catgctctcc tcctgctggc ctctaggacc tctactccat
      541 gatgaaaatc cactacctgg attcccactt caggcgccac cctcctgccg gcaggcaggc
      601 agccgggcga tctccacgcc cggcaggatc ccaccccgcg ggatcaccgg cgcgttgacg
      661 ctgccccgtg aaagtgttac aatgagcgcg tcggccgacc ggccgctcaa cacgcgccgc
      721 ctggggcgaa gccttctttc ggtgttctac cccaacacgc tggagagcac cgcggaggga
      781 cgcccaatag gcgcccgcac agaaggagct ggcagatgat acgtctcggc atcgtcgact
      841 ttgatacctc gcacgtcgtg cagtttacca agcgcctgaa tcacatcgac atcgcggaag
      901 agcagtgggt cgagggcgcg aagatcgtca tgggctatca gggcacctcg cggatcactc
      961 cgcaggagag gatcgacgag tacaccgccc aggtgaaggc gatgggcgtc gaactggtag
     1021 accgccccga ggagatgatt ggcaaagtag atggcatcct ggttgagtcc caggagggcg
     1081 ccgtccacct ggagcgggcg cgccccttcc tcgaagcggg catcccctgc tacgttgaca
     1141 agcccttcgc cacctccgtg gcggatgcga aggcgattgt cgccctcgcg aacaagcaca
     1201 acgtgcccgt cttctcttcc tcgtcgctgc gctacgcgct cgagatccag aagctgcagg
     1261 aagagggcgt gggcaagatc ttcggcgtgg atgcttacag cccggccagc ctgcacgaat
     1321 acaacccggg cctcttccac tacggcattc atggcgtcga gacgctctac gcgctgatgg
     1381 gccccgggtg cgagtcggtg acgtgcacct tcaccgaggg cggcgaggtg accaccggcc
     1441 tctggaagga tggccggatc ggcgtgatgc gcggcacacg cgccggcagc cattcctatg
     1501 gcttcgtagc gtggaccgag aagggcgtgc gccaggtcac catcgacgcg aactacatct
     1561 atcgcgagct g
//
LOCUS       DAOSCV010001038         1566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53468_length_1566_cov_2.894110, whole genome shotgun sequence.
ACCESSION   DAOSCV010001038 DAOSCV010000000
VERSION     DAOSCV010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53468_length_1566_cov_2.894110"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1566
                     /locus_tag="PLY56_18840"
     CDS             <1..>1566
                     /locus_tag="PLY56_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23589.1"
                     /translation="GETDALDLTDGALCARPDNHLWFDAKSVGFSYAGLQQLSLDLGA
                     EVPIGEVAIRLQGGSPQPGISFPVWVDLVASTDGKEYRRVASFSRWNEGDRERFGVPA
                     EEGKGWVHRLTFGDLRLRARYIGLSLYGAGLMVSDELWVLRGPEDSAPPSGPPVPFCT
                     EGAQLYFHKPVVFFSTNIATPNPVGFLCSLPKDTPIKVALDLPPGVRLVAGTLGGVPV
                     AEVKPEALPGGGTRYLFSTQAGESGKTWGRLYLTGDWRDGQEGELRYRISWEGDQAPE
                     GKQRLRAVRIQPAPQPKQLLTGLGWWSLAETRAWPEAAEAFRTLGFTYVPVFPRWVGD
                     ETLWAFLEELRGQGFQVVAIDSPIHVMLNKNKGNPDLMCQLPDGPGKKFCPSYRGPAY
                     QEEVARLAREAAKAKPNLFAADIEVWGWAGPVDAPKCKRCQADFAASGLKEWKEWQVE
                     KGIAMWKELMGAVREASKAAGGPIPLCGGYDFQPGAPYQGVWSVDRLYPEWMHGSQVS
                     TYTPLEPYHLAMVG"
BASE COUNT          237 a          487 c          559 g          283 t
ORIGIN      
        1 gggggagacc gatgcgctcg acctgaccga tggcgctctt tgcgctcggc cagacaatca
       61 cctgtggttc gatgccaagt cggtcggctt ctcctacgcg gggttgcagc agctctcgct
      121 cgacctgggc gccgaggtgc ccatcggtga ggtcgcgatc cggctgcagg gtggttcgcc
      181 gcagccgggc atctccttcc cggtttgggt ggacctggtt gccagcaccg acgggaagga
      241 gtatcgccgg gtcgcctcct tctcgcgctg gaacgagggc gaccgcgagc gctttggcgt
      301 gcccgccgag gagggaaagg gatgggtcca ccgcctgacc ttcggtgacc tgcgcctgcg
      361 cgcgcgctac atcgggctct cgctatatgg cgccgggctg atggtgagcg acgagctgtg
      421 ggtcctgcgt ggtccggaag attccgcgcc gccttctggg ccgcccgtgc ccttctgcac
      481 cgagggggcc cagctctact tccacaagcc ggtggtcttc ttttcgacga acatcgccac
      541 gcccaacccg gtggggtttc tctgctcgct tccgaaggat acgcccataa aggtggcgct
      601 ggatctgccg ccaggagtgc gcctggttgc tggtacgctg ggtggcgttc cggtggccga
      661 ggtgaagccg gaggccctcc ctggtggcgg cacgcgctat ctcttctcaa cccaggcggg
      721 ggagagcggc aagacatggg gccggctcta tttgaccggc gactggcgtg atggccagga
      781 gggcgaactg cggtatcgta tctcctggga gggtgatcag gctcccgagg gaaaacaacg
      841 cctgcgcgcg gtgcggatcc agccggcgcc gcagccgaag cagctcctca ccggtctggg
      901 atggtggtcg cttgccgaga cgcgagcgtg gcccgaagcg gcggaggcct tccgcactct
      961 cggcttcacc tatgtgcccg tcttcccgcg ctgggtgggc gatgagacgc tctgggcctt
     1021 cctggaggag ttgcgcggcc aggggtttca ggtagtcgcg attgactcac cgatccacgt
     1081 catgctcaac aagaacaagg gcaacccgga tctgatgtgc cagctcccgg atggccctgg
     1141 caagaagttc tgtccctctt accgcggtcc cgcctaccag gaggaggtgg cgcggcttgc
     1201 gcgcgaggcg gccaaggcaa agccgaacct cttcgccgcc gatatcgagg tatggggatg
     1261 ggccggaccg gtggatgccc cgaaatgcaa acgctgccag gccgattttg ccgcgagtgg
     1321 cctgaaggag tggaaggagt ggcaggtcga gaaggggatc gccatgtgga aggagctgat
     1381 gggcgccgtg cgcgaggcgt ccaaggcagc gggtggcccg atccccctct gcggcggcta
     1441 tgacttccag cctggggccc cctaccaggg ggtctggtcg gtggaccggc tctacccgga
     1501 gtggatgcac ggcagccagg tttccaccta cacgcctctc gagccgtacc acctggcgat
     1561 ggttgg
//
LOCUS       DAOSCV010001039         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53782_length_1560_cov_2.881728, whole genome shotgun sequence.
ACCESSION   DAOSCV010001039 DAOSCV010000000
VERSION     DAOSCV010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53782_length_1560_cov_2.881728"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1472
                     /locus_tag="PLY56_18845"
     CDS             123..1472
                     /locus_tag="PLY56_18845"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HOJ23590.1"
                     /translation="MNCIWITVDSFRQDHLRCYRPQGTSDPTGKSLLPHTPNIDRLAS
                     EGLRCDRLRSDALPSVPCRCGIFTGRRVFPRRGVPARQGEEINASGWPTLPDEEVTVA
                     EHLARHGYVCALVSDCCHLMEPGQNFHRGFHCYHWERGQEQDRWQSQPLPPGRLERYL
                     KEGTSLSDPRVRILAQYLKNQMYRQGDEEFQAARVFRRAIEWLERNHTHERFYLHIEC
                     FDPHEPWDAPNRLISLYDPRWGGPHLIYPNLWKRDELTDAEHHHIRACYAAECSLVDH
                     WVGRLMSTVERLGLAENTLLIFLSDHGKIIGEFNHYGMAPEDTSLELNSVPCIIRHPK
                     RELAGGRYAGWLYHTDVTATALSLLGVEPKPLTEGESFWPAAASNAEFRDHAVVCYQH
                     VLSCWQDDWLLLVDTARNTTELYHLGEDPHREKDVAARYPEIRDSLQRRIEAVMAAP"
BASE COUNT          271 a          533 c          490 g          266 t
ORIGIN      
        1 cgcctctccc ccgaatggct gcctgccacc ccttacagga tcccctggtt gggcggcgaa
       61 tagagaaacg atgcggcggg ccggcccggg aagagcccgg gttgaaggag cgcagaggag
      121 tcatgaactg tatatggatc acggtggata gcttccggca agaccacctc cgctgttacc
      181 gcccccaggg cacgtcagat cccaccggca agagcctcct cccccacacc ccgaacatcg
      241 accgtcttgc ctcggagggg ctacgctgcg accgtctgcg tagcgacgcg ttgccatcag
      301 tgccctgtcg ctgtgggatc ttcaccggac ggcgcgtctt tccccggcgc ggcgtgcccg
      361 cgcgccaggg cgaggagatc aacgcttccg ggtggccgac cctccccgat gaggaggtga
      421 ccgtcgccga gcatctagcc cggcacggtt acgtgtgcgc cctcgtgagc gattgctgcc
      481 acctgatgga gccgggccag aacttccacc gcgggtttca ctgctaccac tgggaacgcg
      541 gtcaggagca ggaccggtgg cagagccagc cgctgccgcc cggccggctg gaacgctacc
      601 tgaaggaggg cacctctctc agtgacccca gagtgcgcat cctggcccaa tacctgaaga
      661 accagatgta tcgccagggc gatgaggagt tccaggcggc gcgcgttttc cgacgggcga
      721 tcgagtggct ggagcgcaat cacactcacg agcgcttcta cctgcatatc gagtgcttcg
      781 atccccacga gccgtgggac gcgccaaacc gcctcatcag cctatatgac ccccgatggg
      841 gcgggccgca cctcatctac ccaaacctgt ggaagcgcga tgagctgacc gacgccgagc
      901 atcaccacat ccgtgcttgc tatgcagccg agtgctcact ggtggatcat tgggtgggcc
      961 ggctgatgag caccgtcgaa cgccttggcc tggcggagaa cacgctcctc attttcctct
     1021 ctgaccacgg gaagatcatc ggcgagttca accactacgg catggctccg gaggatacca
     1081 gcctggagct caactcggtg ccgtgcatca tccggcatcc gaagcgtgag ttggccggag
     1141 ggcgttatgc cggctggctc taccataccg atgtgaccgc cacggcgctc tcgcttttgg
     1201 gcgtggagcc gaagccgctc acggaggggg agagtttctg gccggctgcc gcgagcaacg
     1261 ccgagttccg cgaccatgct gtcgtttgct accagcacgt tctctcctgc tggcaggacg
     1321 actggctcct actggtcgat accgcccgga atacgacgga gctctaccat ctgggcgaag
     1381 acccccatcg cgagaaggat gtcgccgccc gctacccgga gatccgcgat tccctgcagc
     1441 gtcggattga ggccgtcatg gcggccccct gagccgtgct cctcgccccg tccctgtcgg
     1501 aaacgggcgg ccggcaccag ccggctttga atgcgcgcgc ggggggccgc ctgccctccc
//
LOCUS       DAOSCV010001040         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_53883_length_1558_cov_3.885562, whole genome shotgun sequence.
ACCESSION   DAOSCV010001040 DAOSCV010000000
VERSION     DAOSCV010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53883_length_1558_cov_3.885562"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="rimM"
                     /locus_tag="PLY56_18850"
     CDS             complement(<1..440)
                     /gene="rimM"
                     /locus_tag="PLY56_18850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOJ23591.1"
                     /translation="MKPEECEVVIGQVAAAHGIHGEVRVRPEPDSIEHFRDLKEVCLR
                     HEGRQWIVAIERSRRHGKEIILTLAGVTTVPAARRLQGALVAIPKAERRELPPGEYFI
                     DDLIGLDVYTTDGRLLGQLEEVIQLPANDVYVVGEYLYPAVRDYV"
     gene            complement(437..853)
                     /locus_tag="PLY56_18855"
     CDS             complement(437..853)
                     /locus_tag="PLY56_18855"
                     /inference="COORDINATES: protein motif:HMM:NF022519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlqD family protein"
                     /protein_id="HOJ23592.1"
                     /translation="MQLRRTVTLKVVVTEKFKEETLKALDEALEQLDQARSQIDFRSR
                     IYLTELQRQDLSQAAEFRRRIEAEKRRQDEMKEQLLKQREEITALELGSEYTQGTLEG
                     FVDIQVGDNLQAKLTDAEIVVKDDQVVELREPQPSA"
     gene            complement(1084..1320)
                     /locus_tag="PLY56_18860"
     CDS             complement(1084..1320)
                     /locus_tag="PLY56_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ23593.1"
                     /translation="MKDLVEYLVKALVDHPEAVEIMASHSREGDVYEVRVDPADVGKV
                     IGKGGKTANALRTLVKAAASKTYGRVSVDIVTDD"
BASE COUNT          243 a          536 c          471 g          308 t
ORIGIN      
        1 acgtagtcgc gaaccgcggg gtagagatac tcgccgacga cgtagacatc attcgccgga
       61 agctggatca cctcttccag ctggccgagc aggcggccat cggtggtgta gacatccagc
      121 ccgatcaggt catcgatgaa gtactcgccg ggcggcaact cgcggcgttc cgccttcgga
      181 atggccacca gggctccctg gaggcgcctc gcggcgggaa cggtcgtcac gccggccagg
      241 gtgaggatga tctccttgcc atgtcggcgc gagcgctcga tggcaacgat ccactggcgg
      301 ccctcatggc gcaggcagac ctctttcagg tcgcgaaagt gctcgatgct gtccggttcc
      361 ggccggacac ggacctcgcc gtggatccca tgcgccgcgg ccacctggcc gatgacgacc
      421 tcgcactctt caggcttcat gccgagggct gtggctcccg aagttcgacg acctggtcat
      481 ccttcacgac gatctctgca tcggtaagct tcgcctgcag attatcgccc acctggatgt
      541 cgacgaaccc ctccagggta ccctgggtgt actccgagcc aagctcaagc gccgtgatct
      601 cctcgcgctg cttgaggagc tgttccttca tctcatcctg gcggcgcttc tccgcctcga
      661 tgcgccggcg gaactcggct gcctggctca gatcctggcg ctggagctcg gtcaggtaga
      721 tgcgcgaacg aaagtcaatc tggctgcgtg cctggtcgag ctgctccagg gcctcatcca
      781 gtgccttcag cgtttcctct ttgaacttct ctgtgacgac gaccttgagg gtcaccgttc
      841 ggcgaagctg catgcgtttc cccgtttcac ttcccgccag gcccgcgggc ccagcgcatt
      901 caatggctgc caagccgccg cgtgtgcgcc ggccagccta tccgagatgc ctaacccaag
      961 cgtcccctgg gtgccctccc tcacaaaccg gttgcggccc cgagactgcc ggggccgcgc
     1021 cagcaatgcg gcctgtccgg tgtctggggc gtgggccacc ccgagggcgc gatacaacct
     1081 gcctcagtcg tccgtaacga tgtcgaccga gacccgcccg tacgttttcg atgctgccgc
     1141 cttcaccagg gtccggagtg cgttggcggt cttcccgcct ttcccgatca ccttacccac
     1201 atccgcggga tcgacacgta cctcgtacac atcgccctca cggctgtgcg atgccatgat
     1261 ctcaacagcc tccggatgat ccacgagcgc cttgaccagg tattcgacca ggtctttcat
     1321 ctcatctccc aagtagcctg ccaccggtat gcctcgtcag agggggcagg ctgcggagct
     1381 cgtgcgggct gtgagacgtc aggaagcctg ggccttcgcg gcgcgctttg cctccgcgaa
     1441 gcggctcaga atgccctgtt gcctgagcag cgagcgtgcg gtatcggtgg gctgggcacc
     1501 cttgctcagc cattccagcg cccggtcctc cttgatgacg accgtagcgg gttcggtg
//
LOCUS       DAOSCV010001041         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_54747_length_1541_cov_2.447510, whole genome shotgun sequence.
ACCESSION   DAOSCV010001041 DAOSCV010000000
VERSION     DAOSCV010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54747_length_1541_cov_2.447510"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1541)
                     /locus_tag="PLY56_18865"
     CDS             complement(<1..>1541)
                     /locus_tag="PLY56_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23594.1"
                     /translation="LFPQGYFVGDAWQGHVHVAEYTRDGKPNRACRLSGTREEVGRQA
                     GLWVTSDSVPVKPATRYVVRLRAKGERIVSQGGVSRASLVVDVGQPKEEQLVLYSHYE
                     SGEGHLVEAEQATPVNLLPAMKGRAPKQVPIIVCYASEAYENQEVVAANAEMVIGAGI
                     NWLWGPNESEIARRVRPHGVKFVWHIPRDGYSQEPVDPEYLRRHPEHAAVQRNGVRST
                     RQICPTILLDTPNEFVPKLSAWLTQRLRENPYEMVDWDHEFPLTRNDSVCLCERCRKA
                     FAAEAGLPAVPSLKEVFEQHEDAWVNFRCGQNARMARVIRDAVKAANPEIPFSVYSGY
                     ESARTRSTYGVDWAKMRDAIDWGIAGYNGARGTTRGTMDALGKVPFTTGCMYAEQRFR
                     AEYPYPTPSSWRIRLMRAVLDCDGMGFLVWYLPVLDGGGYWGISWVSALVADYERFFT
                     RFDRRDDLATVEPTMDESTFAVLTHGQERLILLMNPGGSPQKVTLALREVPAGSRLVE
                     YETGR"
BASE COUNT          268 a          559 c          450 g          264 t
ORIGIN      
        1 tgccggcccg tttcgtactc gaccaaccga gacccggctg gcacctcacg cagggcgagc
       61 gtcaccttct gaggcgatcc gcccgggttc atcaggagga tcaggcgctc ttgcccatgc
      121 gtgagcaccg cgaaggtgct ctcatccatc gtgggttcca cggtcgccag gtcatctcgc
      181 cggtcaaagc gcgtgaagaa gcgctcgtaa tcggccacga gcgccgagac ccaactgatc
      241 ccccaatagc cgccgccatc cagaacaggc aggtaccaga ccaggaagcc cattccatcg
      301 caatcgagca cggcgcgcat caggcggatt cgccagctgg atggcgtcgg atagggatac
      361 tcggcgcgga agcgctgctc ggcgtacatg caccccgtgg taaacggcac ctttccgagg
      421 gcatccatcg tgccgcgggt cgtcccccgt gccccgttgt aaccggcgat tccccagtcg
      481 atcgcgtcgc gcatcttcgc ccaatcgacg ccataggtag accgcgtgcg cgcgctctca
      541 tatccgctgt aaacgctgaa cggaatctcc gggttggcgg ccttcacggc atcccggatc
      601 acccgcgcca tgcgcgcgtt ctgcccgcaa cggaagttca cccaagcatc ctcatgctgc
      661 tcgaacacct ctttgagcga gggaacggcg ggcaggcccg cctccgccgc gaacgccttc
      721 ctgcaccgct cgcagaggca caccgaatcg ttacgggtca gggggaactc gtgatcccaa
      781 tcgaccatct catacgggtt ctctcggagg cgctgcgtca gccaggcact cagcttcggc
      841 acaaactcgt tcggcgtgtc gaggaggatg gtcggacaga tctggcgcgt gctacgcacc
      901 ccattccgct gcaccgcggc atgctcggga tggcgccgga gatactccgg atccaccggc
      961 tcttgggagt agccgtcgcg cgggatgtgc cagacaaact tgaccccatg aggccgcacc
     1021 cggcgggcga tctcgctctc gtttggaccc cacagccagt tgatgccggc accaatcacc
     1081 atctcggcat tcgccgcgac cacctcttgg ttctcgtagg cctcgctcgc gtagcacacg
     1141 atgatcggca cctgcttcgg ggcgcgtccc ttcatcgctg gcagcagatt caccggcgtt
     1201 gcctgctcgg cctcgacgag gtgcccctcg ccactctcat agtgcgagta gagaaccagc
     1261 tgctcttcct ttggctggcc gacatccacc acgagagatg cccggctcac accgccctga
     1321 gagacgatcc gctcgccctt cgcgcgcagg cgcaccacgt agcgcgtcgc cggcttcacc
     1381 ggaaccgagt cgctcgtcac ccaaagcccc gcctgccggc ccacctcctc gcgcgttccg
     1441 gagaggcggc aggcgcgatt cggcttgcca tcccgcgtgt actccgccac gtgcacatgg
     1501 ccctgccagg cgtcgcccac gaagtatccc tgcgggaaca g
//
LOCUS       DAOSCV010001042         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_54795_length_1540_cov_2.983165, whole genome shotgun sequence.
ACCESSION   DAOSCV010001042 DAOSCV010000000
VERSION     DAOSCV010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54795_length_1540_cov_2.983165"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLY56_18870"
     CDS             <1..1110
                     /locus_tag="PLY56_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ23595.1"
                     /translation="FITFDHCQRIQKAGIKGAYIRSVLTCKTRHGVCTKCYGADLSTG
                     KPVSIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGEDITHGLPRVEELFEARKPKGL
                     AVITEILLTLVPRPEAKRTLLALFPHLEDAARAVSETIAARIVPATLEFLDQGTARVV
                     EEYAHAGLPTNVEAVLLIEQDGAPSEVDADIGRIAAVCRHAGASEVRVAATAEEAAAL
                     LTARRCALAALARLRPTTILEDASVPRSRLAEMVQAIQEAARRHTVRVCTFGHAGDGN
                     LHPTCLTDARDTDEIARVEEAFAEIFAKALELGGTITGEHGVGAAKAGYLEARVGPGA
                     LALMKRLKEAFDPQGILNPGKIFAEGPRRRMTVSR"
     gene            1221..>1540
                     /locus_tag="PLY56_18875"
     CDS             1221..>1540
                     /locus_tag="PLY56_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ23596.1"
                     /translation="MRCGFCLAACPTYRHLGRETHSPRGRLALVKAATEGQLDPVAAL
                     RQPLDLCLGCRGCEPVCPAGVVYGHVLEEARAALAQRNRPSGLKRWGQRFLFEWLVPN
                     TRLLR"
BASE COUNT          247 a          545 c          533 g          215 t
ORIGIN      
        1 ttcatcacgt ttgaccactg ccagcgtata cagaaagcgg gcatcaaagg cgcgtatatc
       61 cgctcggtgc tgacgtgcaa aacgcgccac ggcgtgtgca ccaagtgcta cggcgcggac
      121 ctgtcgaccg gcaagccggt gtccatcggc gaagcggtgg gcatcattgc ggcgcagtcc
      181 atcggcgagc cgggcacgca gctgaccatg aggacgttcc ataccggcgg cgtggcgggc
      241 gaggatatca cgcacggtct gccgcgcgtg gaagagctgt ttgaggcgag gaagcccaag
      301 ggcctcgcgg tgatcaccga gatcctcctc acgctcgttc cgaggcccga agcaaaacgc
      361 acgctcctcg ccctcttccc ccacctggag gacgccgcgc gcgccgtcag cgagacaatc
      421 gcggcgcgca tcgtgcccgc gacgctggag ttcctcgatc agggaaccgc ccgcgtcgtc
      481 gaggagtacg cgcacgccgg tcttcccacc aacgtggagg cggtgctcct catcgaacaa
      541 gatggcgcgc cctcggaagt ggatgccgat atcggccgga tcgcggcagt ctgccggcac
      601 gcgggcgcgt ccgaggtgcg cgtcgccgcg accgcggagg aggccgcggc gctgctgacg
      661 gcacggcggt gcgctctggc ggcgctcgcc cggctacgcc cgactacgat cctcgaagac
      721 gccagcgtgc cgcgctcgcg cctggccgag atggtccagg cgatccagga agccgcgcgc
      781 cggcacaccg tgcgggtctg caccttcggg catgccggcg atggcaacct ccaccccacc
      841 tgcctcacgg acgcgcgcga cacagacgaa attgcccgcg tggaggaggc gttcgcggag
      901 atcttcgcaa aggcactgga gctcgggggc accatcaccg gcgagcatgg cgtcggcgcc
      961 gccaaagccg gctacctgga ggcgcgcgtg gggccagggg ccctcgcgct gatgaagcgc
     1021 ctgaaggagg cgttcgaccc gcaggggatc ctcaacccgg ggaagatctt cgccgagggg
     1081 ccgcgcagaa gaatgacggt gagccggtga gcacccacgg atcggggcaa cagcttcagg
     1141 gcgcggactc cagcgcatcc ttccgccgag agggcgaaca gcctctcgct ctcaccaccc
     1201 ctctctcgct cgattcctgc atgcgctgcg gcttctgcct ggcggcctgc cccacctacc
     1261 gacatctggg gcgcgagacg cattcgccgc gcgggcgcct ggcgctcgtc aaggcggcta
     1321 ctgagggcca gctcgacccc gtggcggccc tccgacagcc gctcgacctc tgcctgggct
     1381 gccgcgggtg cgagccggtc tgcccggcag gcgtcgtcta cggccacgtc ctggaggagg
     1441 cgcgcgccgc gctcgcccag cgaaaccggc catccgggct gaagcggtgg gggcagcgct
     1501 tcctcttcga gtggctggtg ccaaacacgc gcctgctccg
//
LOCUS       DAOSCV010001043         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_54993_length_1536_cov_3.853477, whole genome shotgun sequence.
ACCESSION   DAOSCV010001043 DAOSCV010000000
VERSION     DAOSCV010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54993_length_1536_cov_3.853477"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..1161)
                     /locus_tag="PLY56_18880"
     CDS             complement(202..1161)
                     /locus_tag="PLY56_18880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOJ23597.1"
                     /translation="MSLVSIVIPTHNCAEYLGGAIRSALRQTHRHIEVIVVDDGSTDD
                     TAQVVRAFGDAVRYLYQPNAGPSAARNLGLAHARGEYVAFLDADDEVRPEWVTMLRKS
                     LHASAFKARFAVCDAWLWDGVNCIDRYRVSEPPSGLRGMDRLLDDNRPYVAILAPRDL
                     LQSVGGFRQGLWVNEDFDLWLRLLVGGYGYSFVAEPLYLYRVRQESLSDDCRRRLEAA
                     CGLYREALATMPLSLGQRRRMAYLLWRERARVSWFDAQAARSKGKRIRWAVHAAESAS
                     AQAVRLLLRPHFTLQRAWRRASERGSMCQCALSVLTSRPGGNA"
     gene            complement(1201..>1536)
                     /locus_tag="PLY56_18885"
     CDS             complement(1201..>1536)
                     /locus_tag="PLY56_18885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ23598.1"
                     /translation="FGSRLMPYSYVFAPEYARFSPIHLLILSVVEEIQGQGLAEEHDF
                     GRGDEEYKLSWTQKVRPLERIVIARRSLRGRLAWWWAVEAKPALWRHPRLHVAVREMR
                     TRLRRYRRG"
BASE COUNT          282 a          539 c          473 g          242 t
ORIGIN      
        1 cgctgccgtt gctgccagcc ggccggtgag ggtccaccgc gctcgccagt aggggctttg
       61 cctgtcatcg cggggaacca accggcttcg gagataccgc ctatgctccc cgcgcgcagg
      121 cagtgccaga gaactgccgc ccggctgtgg gcagcgcgag ataccgcgcg gcccggtgcc
      181 agaactgacc ctactgcctt cctatgcgtt gccgcccggc cgcgaagtga gaacactcaa
      241 tgcgcattgg cacatggagc ctctctcgct cgctctacgc catgcgcgtt gcagggtgaa
      301 gtgggggcgg agaagaaggc gcacagcctg cgccgaggcg ctctccgcgg catgcaccgc
      361 ccatcggatg cgcttccctt tcgaacgcgc cgcctgggca tcgaaccacg agacgcgcgc
      421 gcgttcgcgc cagagaaggt aggccatccg gcgacgctgg cccaggctga ggggcatcgt
      481 tgccaacgct tcgcgataga gcccacacgc ggcttccagc cgccggcgac aatcatcgct
      541 taggctctcc tggcgcacgc ggtataggta gagcggctcc gccacgaagg aatagccgta
      601 tccccccaca agaaggcgca gccacagatc gaagtcctcg ttcacccaca ggccttgccg
      661 gaagcccccc acggattgga gcagatcccg aggggccagt atcgccacat agggccggtt
      721 gtcatccagc agccggtcca tcccgcgcaa tccggacggc ggctcggaaa ctcggtagcg
      781 gtcgatgcag ttcacgccgt cccatagcca ggcatcgcag accgcgaagc gcgctttgaa
      841 ggctgatgcg tggagtgact tgcgcagcat cgtcacccac tccgggcgca cttcatcatc
      901 ggcatcgagg aaggccacat attcgccccg ggcatgcgcc aggcccaggt tgcgcgcggc
      961 agacgggcca gcgttcggct ggtagagata gcgcacggcg tcaccaaacg cccggaccac
     1021 ttgagccgtg tcgtccgtcg agccatcatc caccacgatg acctcgatgt ggcggtgcgt
     1081 ttgccgcaag gcagagcgaa tcgcgccgcc gaggtactca gcgcagttgt gcgtcgggat
     1141 cacgatgctg actagtgaca tttccgccgc ccctccaaga ggttgtcccg cttcggacgc
     1201 tcaccccctc cggtaccgcc ggagccgcgt cctcatctct cgcaccgcga cgtgaagacg
     1261 cgggtggcgc cacagcgccg gtttggcttc caccgcccac caccaagcca gtcgcccgcg
     1321 aagagagcgc cgggcgatga caatccgctc caaagggcgc accttctgcg tccacgagag
     1381 cttatactct tcgtctcctc gcccgaagtc atgctcctct gcgaggccct gcccctggat
     1441 ctcctccaca accgatagga tcagcaggtg gatcgggctg aaccgggcgt actccggtgc
     1501 gaagacatac gagtagggca tcaagcgcga cccaaa
//
LOCUS       DAOSCV010001044         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55108_length_1534_cov_2.554429, whole genome shotgun sequence.
ACCESSION   DAOSCV010001044 DAOSCV010000000
VERSION     DAOSCV010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55108_length_1534_cov_2.554429"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLY56_18890"
     CDS             complement(<1..263)
                     /locus_tag="PLY56_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ23599.1"
                     /translation="MKWSWRVGQIAGIGVHIHATFFLLVGWVALSHWTREQTAGAAIS
                     GVLFILALFACVVAHELGHALAARRYGIATRDITLLPIGGVARL"
     gene            complement(408..1181)
                     /gene="hisA"
                     /locus_tag="PLY56_18895"
     CDS             complement(408..1181)
                     /gene="hisA"
                     /locus_tag="PLY56_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007276806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HOJ23600.1"
                     /translation="MRFRPCIDLHQGKVKQIVGGTLRDDSEPSTNFVSAHPPAYYARL
                     YRDDGLEGGHVIMLGPGNEEAATEALGAAPGWLQVGGGINADNAPLWLERGASGVIVT
                     SYVFREGRVDLERLRQLVTAVGRERLILDLSCRKRDGRYWIVTDRWQRFTEAAVNRDT
                     LAWLAEYCAEFLVHAVDVEGLRQGIDEELVEILAEGAEIPTTYAGGVRSLDDLERIHA
                     LGAGRIDATVGSALDLFGGTLPYRDVVALDRKWRAGAGT"
     gene            complement(1238..>1534)
                     /locus_tag="PLY56_18900"
     CDS             complement(1238..>1534)
                     /locus_tag="PLY56_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HOJ23601.1"
                     /translation="GIADDLLTTVVMALPEETPVVIAPAMNTRMWQNPIVQRNVETLR
                     ALGKYHFVLPRVAELACGDVGTGALAEPVEIFHETLRIAASRASDGGPAEHPAS"
BASE COUNT          244 a          552 c          505 g          233 t
ORIGIN      
        1 agccgggcaa cgcctccgat ggggagcagc gtgatgtcgc gcgtggcgat gccgtaacgg
       61 cgcgcggcca gggcatgtcc cagctcgtgc gccaccacgc aggcaaagag ggccaggatg
      121 aagagcaccc cggagatcgc cgcgccggcg gtctgctcgc gggtccagtg cgacagcgcg
      181 acccacccca ccagcaggaa aaaggtggcg tgaatgtgaa cgccgatgcc ggcgatctgc
      241 cccaccctcc aggaccactt catccctaga cccccctccc gttccgttgc attgccgcca
      301 tgcgccgcgt ggcctggcct gtgccctgac aaatccgcgg cttcgcgtct acttcaagaa
      361 caaggagtgt tttgatccgg ttccggctcc ctcgccggag ccggcgctca ggttccggcg
      421 ccggcgcgcc acttgcgatc gagcgccacc acgtcgcggt aaggaagcgt gcctccaaag
      481 aggtcgaggg cggaacccac agtcgcgtca atccgcccgg cccccaaggc gtggatccgc
      541 tcgaggtcgt ccaggctgcg cacgcccccg gcataggtgg tggggatctc cgcgccctct
      601 gccagaatct cgacgagctc ctcgtcgatc ccctggcgca gtccctccac gtccacggcg
      661 tgaacgagga actccgcgca gtattccgcg agccaggcga gcgtgtcgcg gttgacggcg
      721 gcctcggtga agcgctgcca gcggtcggtg acgatccagt agcggccatc gcgcttgcga
      781 cagctcaggt ctaagatcag gcgctcgcgg ccgacggctg tgacgagctg gcgcaggcgc
      841 tccaggtcca cgcgcccctc gcgaaagacg taggatgtaa cgatcacccc ggacgcgccg
      901 cgctccagcc agagcggcgc gttgtcggcg ttgatcccgc cccccacctg cagccacccc
      961 ggcgcagccc ccagcgcctc cgtggcggct tcctcgttgc cgggcccgag catgatgacg
     1021 tgcccgccct ccaggccgtc gtcgcgatag agccgcgcgt agtaggccgg gggatgcgcc
     1081 gagacgaagt tcgtggacgg ctcgctatcg tcgcgcagtg ttcccccgac gatctgcttc
     1141 accttcccct ggtgcaggtc gatgcaaggt cggaatctca tgataacagg ttgcacgccc
     1201 gaacgtctgg ttcctggccg gcgcggcccg gcaaggctca ggaagcggga tgctcggcgg
     1261 ggccgccgtc cgaggcccga ctcgcggcga tccgcagcgt ctcgtggaag atctccacgg
     1321 gctcggccag tgcgccggtg ccgacgtcgc cacacgccag ctccgccacg cgggggagga
     1381 cgaaatgata cttcccgagg gcgcgcagcg tctcaacgtt gcgctgcacg atcgggttct
     1441 gccacatccg ggtgttcatc gccggggcaa tgacgacggg cgtctcctcc ggcagcgcca
     1501 tcaccaccgt cgtgagcagg tcatcggcga tccc
//
LOCUS       DAOSCV010001045         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55139_length_1533_cov_8.060893, whole genome shotgun sequence.
ACCESSION   DAOSCV010001045 DAOSCV010000000
VERSION     DAOSCV010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55139_length_1533_cov_8.060893"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PLY56_18905"
     CDS             <1..461
                     /locus_tag="PLY56_18905"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23602.1"
                     /translation="FGPEASGHYFFRALSGGDDGLYCALILAGLVARAGAPLSELARD
                     LPRYATTSDIRVRHTGDPTALLERIAASFPGESVCRLDGVRVQFPEGWGLIRPSVTEA
                     ALTLRFEGRTSEALRRVVQEFVAVAPELGPWIEPFANPSSRPRPGSTRTP"
     gene            556..1320
                     /locus_tag="PLY56_18910"
     CDS             556..1320
                     /locus_tag="PLY56_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ23603.1"
                     /translation="MNEAYRGVLEQLVAMSARLGEPERDLVILGEGNTSAAVDDDIFL
                     IKASGRSLVGCGPEAFVAVRRPAVMELLQRETATDEEIKAGLRAAAVDPEAAPPSIEA
                     TFHALLLGLEGVRFVGHTHPTVVNALLCSRDAEELFAGSLFPDQIVLCGVAPLLVPYT
                     DPGLPLARAIREGVEEYHARHGVLPKVIFLRNHGLIALGATPGEVESITAMCVKSCRV
                     LAGTMAFGGPRFMSPENVARIYSRPDEALRRKLITK"
BASE COUNT          233 a          484 c          556 g          260 t
ORIGIN      
        1 tctttggccc cgaggcgagc ggtcactact tcttccgcgc gctctcgggg ggcgacgacg
       61 gcctctactg cgccctgatc ctggccggcc tggtggctcg cgcgggcgcg ccactctcgg
      121 agctggcgcg agacctcccg cgctacgcta cgacctccga tattcgggtg cggcatacgg
      181 gcgacccgac cgcgctgctg gagcgaatcg ccgcttcctt cccgggggag agcgtctgcc
      241 ggctggatgg cgtgcgcgtt cagttcccgg aggggtgggg cctgatccgg ccctcggtga
      301 ccgaggcggc gcttaccctg cgcttcgagg ggcgcacgtc cgaggcgctg cggcgcgttg
      361 ttcaggagtt cgtggcggtg gcgccggaac ttggcccctg gatcgagccg tttgcaaatc
      421 cgtccagcag gccccggccc gggtcaacga gaactccata ggcgagaagc gccgggcatg
      481 ccgcgagcgt cacgtccgga cggtggcggt tgggctggac cgggtgaggg cagcctgagg
      541 gaatggaaga caacggtgaa cgaagcatat cgaggcgtat tggaacagct ggtagcgatg
      601 tctgctcgcc tgggcgagcc ggagcgcgac ctggtgatct tgggagaggg gaacacctcg
      661 gcggcggttg acgacgatat cttcctgatc aaggcgagcg ggcgcagcct ggtcggatgc
      721 ggacccgagg cgtttgtcgc cgtgcgccgc ccggcggtga tggagctgtt gcagagggag
      781 acggcgacgg atgaggagat caaggcgggc ctgcgcgccg cggccgtcga tcccgaggcg
      841 gcgccgccgt ccatcgaggc cactttccac gcgctgcttc ttgggctgga gggcgtgcgc
      901 tttgttgggc acacccatcc cacggtggtg aacgcgctcc tctgctcgcg tgacgccgag
      961 gagcttttcg cggggagcct cttcccagac cagatcgtcc tctgtggcgt ggcaccgctg
     1021 ctcgtgccct acaccgatcc gggcctcccg ctggcgcgag cgatccgcga gggcgtcgag
     1081 gagtaccacg cgcggcatgg cgttctgccg aaggtgattt tccttcgcaa ccacgggctg
     1141 atcgccctcg gtgctactcc cggcgaggtg gagagcatca ctgcgatgtg cgtgaagtca
     1201 tgccgcgttc tcgccggcac catggcgttc ggcgggcccc gcttcatgag ccccgagaat
     1261 gtggcgagga tctactcgcg ccccgacgag gcgctgcgcc gcaagctgat taccaaatag
     1321 cgagcgggag gcatccctct cccagcgtgc cctgagattc cgacataggg aatccgaagc
     1381 ctcggaggca gcgtgcaacc cgtcgcgcgg cgataaatca ccgcgctgct tgcgagcgaa
     1441 gccctgccgg gctgggttgg ggatactccc agatgtctca aaacggcggt ggctgggagg
     1501 agaagccctg aaagggcttt gccggaaagc agc
//
LOCUS       DAOSCV010001046         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55157_length_1533_cov_2.878214, whole genome shotgun sequence.
ACCESSION   DAOSCV010001046 DAOSCV010000000
VERSION     DAOSCV010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55157_length_1533_cov_2.878214"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1533
                     /locus_tag="PLY56_18915"
     CDS             <1..>1533
                     /locus_tag="PLY56_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23604.1"
                     /translation="GVSITEISAPGQVMTANLYVVPPMILSCRPKPNFSTESVTPTFR
                     VSFSDAIDPASILVELDDRIVVSEANLSRYYNAATRELTYRTNQLAFGQHVIRVSVKN
                     KTKTVTESTGNRGFRVNFRTIPAGLRMVSVPYLLAAPENTPQSVFGATAGMLARWVPS
                     KQDYRYWPQHPEAGFYPPPDDPQNPSFALSTRPAGLGFWIKLSTDTPVRVNGDQIEDV
                     AVRIPLSAGWNMIGSPFPFPVDWNGCQVEYQGQIKPLSEAVDAGWISGTLYTYEPGGY
                     TWVNAPAGQLKPWEGYWVKAKVNCWLQVPPVASGVIGLGESRSRDDMALEERGRATAK
                     EWRLRLSVSAGSESEDRYNFIGVSESARDGADSRDVEEPPAPGDGVVSLYLVEGGRRL
                     AQDLRSAANGGEQVWNVEVALPVARQQVTLSWPDLSALPRDMDAYLEDEATGRIVAMR
                     TRTAYSFDSGEGGVRRFRITVRPRGGSSLAITELSVMRTRGVPSVRVNISRDAMIRVE
                     AV"
BASE COUNT          272 a          459 c          543 g          259 t
ORIGIN      
        1 cgggagtctc catcaccgag atttcggcgc ccggtcaagt gatgaccgcc aacctctacg
       61 tcgtgccgcc gatgatccta tcgtgccggc ccaagccgaa cttctcgacg gagagcgtga
      121 cgccgacgtt ccgagtctcg ttcagtgacg cgatcgaccc ggcatcgatc ctggtggagt
      181 tggatgaccg gatcgtggtc agcgaagcca atctcagccg gtactacaac gccgcgacac
      241 gcgagctgac ctatcggacg aaccaactgg cattcggcca gcacgtgatc cgcgtctcgg
      301 tgaagaataa gactaagacg gtgacggaat cgacggggaa ccggggattc cgagtgaact
      361 tccggacgat cccggccggg ttgcggatgg tctcggttcc gtatctgctc gccgcgccgg
      421 agaatactcc gcagagcgtc ttcggggcga cagccggaat gctggcgcgc tgggtgccat
      481 cgaagcagga ctatcgctat tggccgcagc atcccgaggc cggcttctat ccgccgccgg
      541 atgatccgca gaacccgtcg ttcgccttga gcacgcgtcc agccgggctt ggcttctgga
      601 tcaagctctc taccgatacc ccggtccggg tgaacgggga tcaaatcgag gatgtggcgg
      661 tccggattcc gctgagcgct gggtggaata tgatcggcag ccccttcccg ttcccggtgg
      721 actggaacgg gtgccaggtg gagtaccagg ggcagatcaa gccgctcagc gaggcggtgg
      781 atgccgggtg gatcagtggc accctctaca cctacgagcc cggaggctac acctgggtga
      841 atgcgcccgc cgggcagctg aagccgtggg aaggctactg ggtcaaggcg aaggtcaact
      901 gctggctgca agtgccgccg gtggcttcgg gcgtgattgg cctgggtgag agccgctccc
      961 gggatgacat ggcactggaa gagcgtgggc gggccaccgc caaggagtgg cgcctgcgcc
     1021 tcagcgtcag cgccggatcc gagagcgagg atcggtataa cttcatcggg gtgagcgaga
     1081 gcgcgcgcga cggcgccgat agtcgggatg tggaggagcc tcccgcccca ggcgatggag
     1141 tggtgtcgct ctacctggtg gagggcggac gccgcctggc gcaggacctt cggagcgccg
     1201 ccaatggagg cgagcaggtt tggaacgtgg aagtggccct gccggtcgca cggcagcagg
     1261 tgacgctctc atggccagat ctctcggccc tcccgcgcga catggatgcg tatctcgagg
     1321 acgaggcaac cgggcggatc gtggcgatgc gcacccgcac ggcctattcg ttcgacagtg
     1381 gagagggcgg cgtgcgccgc ttccggatca ctgtccggcc gcgtggcgga agctcgctgg
     1441 cgatcacgga actgtcggtc atgcgaacgc gcggagtgcc ttccgtgcgc gtgaatatca
     1501 gccgtgacgc gatgatccgg gtggaagcgg tca
//
LOCUS       DAOSCV010001047         1526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55497_length_1526_cov_4.460911, whole genome shotgun sequence.
ACCESSION   DAOSCV010001047 DAOSCV010000000
VERSION     DAOSCV010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55497_length_1526_cov_4.460911"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLY56_18920"
     CDS             complement(<1..368)
                     /locus_tag="PLY56_18920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ23605.1"
                     /translation="MQLRRYILVPILTGIALAAPGWGLFGGSSPLVTPAEAANRMMLP
                     TRNTITSRFGPRRLGSHHGVDLRAPMGTPVKASAAGRVIFAGWKNSIYGNMVAIQHPN
                     GLVTRYAHLSKVSVRPGQRVA"
     gene            1024..>1526
                     /locus_tag="PLY56_18925"
     CDS             1024..>1526
                     /locus_tag="PLY56_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOJ23606.1"
                     /translation="MSVAALGGGTGLSSLLQGLKHVVDDLTAIVAVSDDGGSSGRLRA
                     DLGCIPPGDIRNCLVALAEAQPLMKELFQYRFHAPGKGLDGHAFGNLLIAALADITGS
                     FETAISETGKVLAIRGRVLPLSLARIDLRGHMGDGVWIRGESAITAYPAPIERVETPH
                     EVWKGYL"
BASE COUNT          258 a          475 c          510 g          283 t
ORIGIN      
        1 gccacacgct ggcccgggcg caccgagacc ttcgacaggt gcgcgtagcg cgtcaccagt
       61 ccgttcggat gctggatggc aaccatgttg ccatagatgc tattcttcca tccggcaaag
      121 atgacccggc ccgcggcaga ggccttcacc ggcgtgccca tcggtgctcg caggtccacg
      181 ccgtggtgag agcccaagcg tcgcggccca aagcgcgaag tgatagtgtt gcgggtcgga
      241 agcatcattc ggttcgcggc ctcggctggg gtcaccagcg ggctgctccc gccaaaaagg
      301 ccccaaccgg gggctgccaa agcgattcca gtcaaaatcg gtaccagaat atatcgtcga
      361 agctgcaagg cttttccttt ccgtcaaggc tgccgttcag gacccctctg ccaggcacgc
      421 gccatcgaca cgtgccaatg cccttcgcgg aatcacagac ccattagtgg aagtggcccg
      481 aaaccccggc agcctcgtag atcgtgcgtc gtcggcgtcc cttcacgctt cgtggccggc
      541 aatcgccggc ggctcggggc gcctatcgac gttgtcttgc tcgcctggtt tgccatatcg
      601 ccgcccgaag gcgcttggtg atccccgtcg cggggactgc ctggcatggc cgggcaacgc
      661 tggtcacgct ggcccgcatg accctgatcg cgttgctgcc atattcgcgg cggcgaacga
      721 atggaacgaa caatcgtgcg ttatagctcc caggacacgc ctcctcgctg gaaggtttcg
      781 ccgtggatca aaatcctgtt ttttcgcgac agggcacggg gtatgcagga gatagcaccg
      841 gagatgacgg gagcatgcgt tcgagcgtgc ctggcgatgc ccggtggctc catgcaacgc
      901 cccttggggc attgggcgcg gagaccggcg atccgctgcg ggtcggatgt gggtggctgt
      961 gcccgtctcc gtgacgggcg gtgtggggtg tgaacggaga agagggtgga gagtggaagg
     1021 aacttgtctg tcgccgctct ggggggaggg acgggcctat cctcgctgct gcagggcctg
     1081 aaacacgtcg tcgatgacct caccgcgatc gtcgctgtct cagacgacgg gggcagctcc
     1141 ggacgcctgc gcgccgatct cgggtgcatt ccaccggggg acatccgcaa ctgcctggtg
     1201 gcgttggccg aggcgcagcc cctgatgaaa gagctgtttc agtaccggtt tcatgcccca
     1261 ggcaagggtc tggatggcca tgccttcgga aacttgctca tcgccgcgct cgccgatatt
     1321 accggctcct tcgagacggc catcagcgag acgggcaaag ttctggcgat tcgcgggcgg
     1381 gtgctccccc tgagcctggc gcgaatcgac ctccgcggcc atatgggcga cggcgtctgg
     1441 atccgcggcg agagcgcgat cacggcctat ccggcgccga ttgagcgcgt ggagaccccc
     1501 catgaggtct ggaaggggta tcttat
//
LOCUS       DAOSCV010001048         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55840_length_1519_cov_4.349727, whole genome shotgun sequence.
ACCESSION   DAOSCV010001048 DAOSCV010000000
VERSION     DAOSCV010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55840_length_1519_cov_4.349727"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLY56_18930"
     CDS             complement(<1..352)
                     /locus_tag="PLY56_18930"
                     /inference="COORDINATES: protein motif:HMM:NF015317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HOJ23607.1"
                     /translation="MDESETGAPAQQEVPEPREVPEGSPTLEGLFEAVLWHSRFLMVV
                     PVIVGILMAAGTFYVACVDTVYLLGLASRIADITLTEEMRQGLRAEIMASVVKVTTIS
                     LIGAALLLFSTGLYQ"
     gene            complement(380..>1519)
                     /locus_tag="PLY56_18935"
     CDS             complement(380..>1519)
                     /locus_tag="PLY56_18935"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ23608.1"
                     /translation="KMHVFPERNLLGFHNVVLHDGYLHFARSQHRNHSLIDDYLPWLR
                     EKLGDPYVDHTDTGLGCNGYAVRPWVYDDRLHPTSWVTTQGIDFLRRRDPARPFFLML
                     SYHRPHPPLDPPRSFLERYLRKELPSLPMGDWVDHELPAHRGLDSPVPRDPEEIDLAR
                     RAYYAQLTHIDHEINRVLIALHEYGLLEDTAILFTSDHGDMLYDHNYIAKSLPYDGSA
                     RVPFILRPPGRGVWDEKPGQEIDAVVELRDVFPTLCDLAGVPIPEGLDGASVLPLLRG
                     EERGWREYLHGEHAAGPRSNHWVTDGREKYVWFSQTGREQLFEIATDPQELRDLAPLR
                     QDRVAYWRRKLMRELEGRPEGFVQEGKLVAGRPVSPALEHAGVGV"
BASE COUNT          262 a          512 c          496 g          249 t
ORIGIN      
        1 gctggtagag gccggtggag aagagaagca gagcggcgcc aatcaggctg atggtggtca
       61 ctttgaccac gctcgccata atctcggcgc gaagcccctg gcgcatctcc tccgtcagcg
      121 tgatgtcggc gatgcgactg gccagcccga ggaggtagac cgtatcgacg cacgccacgt
      181 agaaggtgcc cgcagccatg aggatcccca cgatgaccgg gaccaccatc aggaaccggc
      241 tgtgccagag caccgcctcg aacagtccct cgagcgtggg cgaaccctcc ggcacctctc
      301 gcggctccgg cacctcttgc tgcgcaggcg cgcctgtctc gctctcgtcc atcaacgccc
      361 cacttcctct cttcgccgcc tacacaccga cgcccgcgtg ctccagcgcc ggcgagaccg
      421 gccttccggc caccaacttg ccctcctgaa cgaagccttc cggacggccc tccagctcgc
      481 gcatgagttt tcgccgccaa taggcgacgc gatcctgccg gagcggggcc aggtcgcgca
      541 gctcttgcgg gtccgtcgcg atctcgaaca gctgctcgcg tcccgtctgg ctgaaccaga
      601 catacttctc ccgcccgtcg gtcacccagt ggttcgaccg cgggccggca gcgtgctcgc
      661 catggagata ctcgcgccag ccacgctcct cgccgcgcag gagcgggagc acgctcgcgc
      721 catccaatcc ctcgggaatc ggcaccccgg cgaggtcgca gagcgtcggg aagacgtctc
      781 gcagctccac gaccgcgtcg atctcctgcc ccggcttctc atcccacacg cctctcccgg
      841 gaggccgtag gatgaacggc acacgtgccg agccatcata cggcagcgac ttggcgatgt
      901 agttgtgatc gtagagcatg tcgccatggt cggacgtgaa gaggatcgcc gtgtcttcca
      961 gaaggccgta ttcgtgcagc gcgatcaaga cgcggttgat ctcgtgatcg atgtgcgtga
     1021 gctgggcata gtaggcacgc cgggccaggt cgatctcctc ggggtcacgc ggcaccgggc
     1081 tatccaggcc gcgatgcgcc ggcagctcgt gatctaccca atcgcccatc ggcagcgacg
     1141 gcagctcttt ccgcaggtag cgctccagga aggagcgcgg cgggtccagc ggcggatgcg
     1201 gccggtgata cgagagcatc aggaagaagg ggcgggctgg gtcgcgccgg cggaggaaat
     1261 cgatcccctg ggtagtcacc cagctcgtcg gatggagccg gtcgtcgtag acccaggggc
     1321 gcacggcgta gccattgcac ccgaggccgg tgtcggtgtg atcgacatag ggatcgccca
     1381 gcttctcgcg cagccagggg aggtagtcgt caatcaggct atggttccgg tgctgactgc
     1441 gcgcgaagtg gaggtagccg tcgtgcagga ccacgttgtg gaagcccagc aggttgcgct
     1501 cgggaaagac gtgcatctt
//
LOCUS       DAOSCV010001049         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55853_length_1519_cov_2.816257, whole genome shotgun sequence.
ACCESSION   DAOSCV010001049 DAOSCV010000000
VERSION     DAOSCV010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55853_length_1519_cov_2.816257"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..762)
                     /locus_tag="PLY56_18940"
     CDS             complement(196..762)
                     /locus_tag="PLY56_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23609.1"
                     /translation="MEIVRRATAQQRHEARVEAARQAALDAVPNPRLAGVLCALEVNG
                     HGGPLGMGGHLGLRATIRAEWARGLGSGRAQAGDGLVWDAAIRAAAHDLLQPGPGHVT
                     RPWEKVAPTAQDVMDLLDRITDPVVLEEARAYARSRIYETARTAHDPAPAVRDWDRVV
                     RAVAERMAAPEVPTEDDPQGPQIKWEVE"
     gene            complement(1056..1436)
                     /locus_tag="PLY56_18945"
     CDS             complement(1056..1436)
                     /locus_tag="PLY56_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23610.1"
                     /translation="MKNNEKELRIMALALPVIEAVPSEQDECWPGDPSHDDHCSLTDH
                     AESVAAVKATEGVSWEFRSWSSTHAAWRSVLDEAVSEFVNAYEDDDEIPIEELERIFV
                     LAYERTADDSDRRTGLWSLICAAA"
BASE COUNT          239 a          518 c          472 g          290 t
ORIGIN      
        1 gtcaagaatt cgtttaaaaa cataaatccc ttttcgtgat ggtggatgca gcgatatgtg
       61 ggcacgtacc ggcacgggaa tgccccggct ctccgccaca gcattccgga ggctccgtac
      121 tacttgcccg ctgatgatcc gctgcaggcc gaactggatg cgtggatgaa agcgcatcgg
      181 cctgggaccg tcgcattact ccacctccca tttgatctgc ggaccctgag gatcatcctc
      241 tgtcggcacc tcaggggccg ccatgcgctc ggctaccgcc cggaccaccc ggtcccaatc
      301 ccggactgcg ggagcaggat cgtgggcggt acgggccgtc tcgtagatcc tggaccgcgc
      361 gtaggcgcgt gcttcctcca ggaccacagg atcggtgatc cggtccagca ggtccatcac
      421 gtcctgagcc gtgggagcga ccttctccca cggccgggtc acgtgtccgg gtccgggctg
      481 caacaggtcg tgggcagcag ccctgatggc cgcgtcccac accagcccgt cgccggcctg
      541 ggcccggccg ctgccgagac cgcgcgccca ctcggcgcgt atcgtggccc gcagcccaag
      601 gtgcccgccc atcccgagcg ggccaccgtg gccgttcacc tccagcgcgc acagcactcc
      661 ggcgagccgt gggttcggca cagcgtcgag ggccgcctgc cgggcggctt cgacgcgagc
      721 ctcatggcgc tgttgcgccg tagcccgacg cactatctcc acggtttcct cggagagtcc
      781 ttctccgaga gattcgcgcg tttctcgcca tgtcatcgtc attgtgattt ttctccttcg
      841 cgcgctctct cccggtcaat tccgggtgcg gtctatcgac ccccgctccc cgcccgtcgt
      901 ggaggcggcg cgtctagggg agctgcccgg atgcgctccg ggcgggcgac ggcggcaagc
      961 tcagggagct gcctgaaggg gttttacggg gttgcgtacc cgccccacct accacggtgg
     1021 acgctccgtc cgcacggaga agcacggagc ggagattagg cggcagcgca gatcagcgac
     1081 cacagcccgg tgcggcgatc cgagtcatcg gctgtccgct cgtaggccag cacgaagatc
     1141 cgctctaact cttctatggg gatctcgtca tcgtcctcgt aggcgttaac gaactcgcta
     1201 acagcctcat cgagcacgga gcgccaagca gcgtgcgtgc tggaccaaga cctgaactcc
     1261 caactcacgc cttccgtggc tttcacggcg gcgacagatt cagcgtgatc tgtcagggag
     1321 cagtggtcgt cgtgggaagg gtcgcccggc cagcattcgt cctgttcgct cgggacggct
     1381 tcgatcaccg ggagcgccag cgccataatt cgaagttcct tctcgttgtt tttcatctga
     1441 tttctcctgc cgttggtagc ggcttacagt tctggcgaag cgctcatgag agcgcctcca
     1501 ccacctccgg tgcgaggat
//
LOCUS       DAOSCV010001050         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55898_length_1518_cov_3.118934, whole genome shotgun sequence.
ACCESSION   DAOSCV010001050 DAOSCV010000000
VERSION     DAOSCV010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55898_length_1518_cov_3.118934"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLY56_18950"
     CDS             complement(<1..298)
                     /locus_tag="PLY56_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ23611.1"
                     /translation="MQRVCVIGLGPIGNRHADMYQECPRAKLVGVCDIRRDRADAAAA
                     RLGVPAFYSVKEMLAALEPDLCSVATGGEEYGSDHYEPTMEALEAGCHVLCEKPI"
     gene            complement(372..>1518)
                     /locus_tag="PLY56_18955"
     CDS             complement(372..>1518)
                     /locus_tag="PLY56_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23612.1"
                     /translation="GHVEGRAGRQGRHIFHTRLRLPHSEVIHPVCLEVRPAFELLSAE
                     LKVSAAFPGEALLEVTLRCNRPERVPPPVEILYAGRTYRSRIGKGEPVSSEPAASGKR
                     GSGDGRRRAPGPSHAGAPRAEVFRFRVKAPERLSPGGIPVRVTLRLLSGPVVLEGQTR
                     LWSLFAAWPDGARAFAARCTPLEIPRNDCLEALFTRTYEGEDAPFLNCWTWYEPEVIN
                     LEHVRERLRGGLFESDVGVLFRIPSEGDNILCVTRWSSHPTEATIPVGRPAEVIYLLL
                     ANVTQNSQTHLAQARVHIEYADGREERIDLRGPGQIDHMLQHYAAANYPQWVGGKLRG
                     YYGHGRASGTHADIVDLHPRYREPIARLRVECLTRETLIGILGVTLL"
BASE COUNT          252 a          555 c          472 g          239 t
ORIGIN      
        1 agatcggctt ctcgcacagg acatggcagc cggcttccaa tgcctccatc gtgggctcgt
       61 aatggtcgct gccatactcc tcgccgcccg tggccacgct gcagagatcc ggctcgagag
      121 cagccagcat ctccttgacc gagtagaacg cgggcactcc caggcgcgcg gcggccgcgt
      181 ccgcccgatc gcgccgaata tcgcacaccc caacgagctt cgctcgcggg cactcctggt
      241 acatatccgc gtgccggttg ccgattggcc ccagcccgat aacgcacacg cgttgcattg
      301 aagcactcct cacctttggg aattgtcacc cagagagatg gttcgctccc ccacccccga
      361 ttcccgcttc ctcaaaggag ggtgacgccc aagatcccga tgagcgtctc gcgcgtcagg
      421 cactccacgc gcaggcgggc aatcggctcg cggtagcgcg ggtggaggtc cacgatatcg
      481 gcatgcgtgc cggacgcgcg gccatgcccg tagtagcccc gcagctttcc gcccacccac
      541 tgggggtagt tcgccgccgc gtagtgttgg agcatgtggt cgatctgccc ggggccgcgc
      601 aggtcgattc gttcctcccg gccatccgcg tactcgatgt ggacccgcgc ctgcgccaga
      661 tgcgtctggc tgttctgcgt gacgtttgcc aacaagaggt agatcacctc ggccggccgg
      721 cccacaggga tcgttgcctc tgtcgggtgg gagctccacc gcgtgacgca gaggatgttg
      781 tcgccctcgg aagggatccg gaagagcacg ccgacatcgc tctcgaagag gcctccacgc
      841 aagcgctcgc gcacgtgctc cagattgatc acctctggct cataccatgt ccagcagttg
      901 agaaacgggg catcctcccc ctcgtacgtg cgcgtaaaga gcgcctccag gcaatcgttg
      961 cgggggatct ccaacggcgt gcagcgcgcg gcaaacgccc tcgcgccatc tggccatgcc
     1021 gcgaagagcg accagagccg cgtctggccc tccagcacca ccgggcccga gaggaggcgc
     1081 agcgtcacgc gcaccgggat cccaccaggc gatagccgtt ccggcgcctt cacccggaac
     1141 cggaacacct ccgcgcgagg agcacccgcg tgggaaggtc cgggagctcg ccgccggcca
     1201 tccccactgc cgcgctttcc cgacgcggcc ggctccgagc tcaccggctc tcccttgccg
     1261 atccgggagc ggtaggtgcg cccggcgtag aggatctcga ccggaggcgg cacccgctcc
     1321 ggcctgttgc aacgaagggt cacctccagc agcgcctctc ccggaaacgc cgcgcttacc
     1381 ttgagctccg cgctcagcag ctcaaacgcg ggccggacct ccaggcaaac cgggtggatc
     1441 acctcagagt gcggcaggcg caggcgcgta tggaagatgt gccggccctg tcgtccggca
     1501 cgcccctcca catgcccg
//
LOCUS       DAOSCV010001051         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_55941_length_1517_cov_4.004788, whole genome shotgun sequence.
ACCESSION   DAOSCV010001051 DAOSCV010000000
VERSION     DAOSCV010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55941_length_1517_cov_4.004788"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            408..656
                     /locus_tag="PLY56_18960"
     CDS             408..656
                     /locus_tag="PLY56_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23613.1"
                     /translation="MARKPPEVLEMTEMIVLHGDSLNGTDAERSAKLERIKEALREKE
                     IDFIDQPEDLQVWVGTADREAVLATIEALGFKPDLYRV"
     gene            complement(704..1375)
                     /locus_tag="PLY56_18965"
     CDS             complement(704..1375)
                     /locus_tag="PLY56_18965"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ23614.1"
                     /translation="MRQNHSAIHYWLACEGVRCLCRLLGGIEVLGRENFPESSRLIVV
                     SNHLSHLDPPLIGGVVPWYTHCMAKEELFKTPFIGWWCRCVGAFPVKRGAADRQALRT
                     ALQVLEMGRPLVLFPEGRRSKDGRLQPGEEGVGLIALRSRVPIIPIGIAGSQSILPPG
                     SIRFRRGHVTIRIGKPFTLDDLYGQTGRPAVIEATRRVMAEIASLLPPEHQPADGIPR
                     TISVA"
     gene            complement(1372..>1517)
                     /locus_tag="PLY56_18970"
     CDS             complement(1372..>1517)
                     /locus_tag="PLY56_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ23615.1"
                     /translation="RIAMAGAVAALVAMGESVIRGAECVDVSFPGFAERLRQLGAAVG
                     EGE"
BASE COUNT          295 a          524 c          480 g          218 t
ORIGIN      
        1 aagccgcgca cctcgagcgg ccttgatccc ggcgacgtgc cagggagcga tcacaccaca
       61 accgataata ccaaaaccca actcctccat gtcactctca ctccttcccg tagccggcag
      121 cgccaagcaa agagagcatc gtggctttca gaaaggctgc cggcaaaaag aatcttcgac
      181 gcgcatccgc cggtctcctg cccgttgcgg gcatcacggc ccgtacaccg gggatggaga
      241 ccccgtgccc cagaaccccc aggcgctctc cacgccccgc cccgtggcgc gcaggagagc
      301 cttggctacg gggcgaatgg ggttcaggaa cgggccgtcg tggcccgcgc cagggaagcg
      361 gagccggtca tgccgggcgc cctgagcccc tggcatggga ggagtgcatg gctagaaaac
      421 cgccagaagt gctggagatg accgagatga tcgtgctgca cggcgactcg ctgaacggca
      481 ccgacgcgga gcgcagcgcg aaactggagc gcatcaagga agcgctccgc gagaaggaga
      541 tcgatttcat cgaccaaccg gaggatctcc aggtgtgggt tggcaccgcc gaccgcgagg
      601 ctgtgcttgc caccatcgag gctctcggct tcaagccaga tctctatcgg gtgtaagctg
      661 cccgtcttat gacccggtgc tccacgcgca ggggatggga agcctacgcg acggagatgg
      721 tcctgggaat cccatccgcg ggttgatgct ccgggggcag gagcgaggcg atctcggcca
      781 tcacgcgccg ggtcgcctcg ataacggcgg gccggcccgt ctgaccgtaa aggtcatcca
      841 gcgtgaacgg cttgccgatc cgaatcgtca cgtggccgcg ccggaagcgg atgctgccag
      901 gcgggagaat gctctgggag cccgcgatcc caatcgggat gatcggcacg cgtgagcgaa
      961 gagcaatgag gccaacgccc tcttcgcccg gctgaagccg gccatccttg ctgcgccggc
     1021 cttccgggaa gagcaccagg gggcgcccca tctccagcac ctgcaacgcc gtgcgcagtg
     1081 cctggcggtc cgccgctccc cgcttcaccg gaaacgcgcc cacgcaccgg caccaccacc
     1141 cgatgaacgg cgtcttgaac agctcctctt tcgccatgca gtgcgtgtac cacggcacca
     1201 ctccgccgat gagtggcggg tccagatggc tgaggtgatt ggagacgacg atcagccgcg
     1261 aggactcggg gaagttctcg cgcccgagca cctcaatccc gcccaggagg cgacagaggc
     1321 agcgaacgcc ctcacacgca agccaatagt ggatcgcact gtggttctgc ctcattcacc
     1381 ttctcccaca gcggccccca gttgccggag ccgctccgca aagccgggga aagagacatc
     1441 gacgcactct gcgccgcgga tcacgctctc ccccatcgcg acgagagccg ccaccgcgcc
     1501 cgccatggcg atgcgat
//
LOCUS       DAOSCV010001052         1516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56015_length_1516_cov_2.387406, whole genome shotgun sequence.
ACCESSION   DAOSCV010001052 DAOSCV010000000
VERSION     DAOSCV010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56015_length_1516_cov_2.387406"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLY56_18975"
     CDS             <1..403
                     /locus_tag="PLY56_18975"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOJ23616.1"
                     /translation="VEVRCEAGLVRLEGRVHTAAERTELDLICERLHESRGGIRAVEN
                     RVEVERRPREDDEEMRAALEQHLAALISDSGGMAGARVQVDVIGGVAYLTGLVESAQD
                     RAFAGQRALEVPGVTAVVNWIDLQGHRYRIR"
     gene            496..>1516
                     /gene="dprA"
                     /locus_tag="PLY56_18980"
     CDS             496..>1516
                     /gene="dprA"
                     /locus_tag="PLY56_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ23617.1"
                     /translation="MATLDEIEPWVRLNRAELMPRKAARLLAEFGTPEAVLSAARAEL
                     ARDRPSTRANCDRLAEAALWDGWPAEQATLQRLGIRVIPLTSDDYPARLRAIPDPPLV
                     LYVRGSLLPEDEYAVAIVGSRRATPYGRQVAADLARGLARAGVTIVSGMARGIDAAAH
                     QGALNAGGRTLAILGCGVDQCYPPEHRGLRDSIAAQGAVISEFSLGAAPDKWRFPARN
                     RIISGLCLGVILIEAPADSGAMITADFALEQGREVFAVPGNIDSFASRGCHQLIKEGA
                     HLIESAADILDALDLSTAALEDAPPQQPSLPFPLSPEAEQVLARLGPQQRHVDRGRGR
                     EVRAGA"
BASE COUNT          224 a          505 c          539 g          248 t
ORIGIN      
        1 cgtggaggtg cgctgcgagg ccggactggt gcgtctggaa ggccgtgtcc acacggctgc
       61 cgagcgtacg gagctcgatc tgatctgcga gcgtttgcac gagagccgcg gcggaatccg
      121 cgcggttgag aaccgtgtgg aggtggaacg gcgcccgcga gaggatgatg aggagatgcg
      181 cgccgcgctg gagcagcatc tcgctgccct gatctccgat agcggcggca tggcgggggc
      241 gcgcgtgcag gtggatgtga tcggcggcgt agcctatctg actggtctcg tggaatcggc
      301 gcaggatcgc gccttcgcgg ggcagcgggc gctcgaagtg cctggggtga cggccgtcgt
      361 caactggatt gacctgcagg gccaccgata ccggatccgt tagcgggcga agcggttgtg
      421 gatgcggggc gcggggcgcc ccctacccga gtcgcgtata acgagcaggg ccgtcgttgc
      481 cagatgggga gagccatggc gacactggac gagatcgagc cctgggtccg cctgaaccgc
      541 gcggagttga tgccgcggaa agcggcgcgg ctcctggcgg agtttggaac ccccgaggcg
      601 gttctgagcg cggcgcgcgc cgagctcgca cgcgatagac cgtccacgcg tgcgaactgc
      661 gaccgtctcg ccgaggcagc cctctgggat ggctggccgg ccgagcaagc caccctccag
      721 cgcctgggca tccgcgtcat tcccttgacg agcgacgact atcccgcccg gctgcgcgcg
      781 attccagatc ccccacttgt cctctacgtc cgcggcagtc tcctgcccga ggatgagtac
      841 gccgttgcca ttgtcggctc gcgccgggcg acgccgtatg ggcgccaggt ggcggccgac
      901 ctggcgcgcg gcctggcgcg cgctggcgtc accatcgtca gtgggatggc gcggggcatc
      961 gatgccgccg cgcatcaggg agcgcttaac gctggcggcc gcaccctggc gatcctcggg
     1021 tgtggcgtgg accagtgcta tcccccggag catcgtggcc tgcgcgattc catcgccgcg
     1081 cagggtgccg tcatctccga gttctccctg ggcgccgccc cggacaaatg gcgttttccc
     1141 gcgcgcaacc gcatcattag cggcctctgc ctgggagtga tactcatcga agccccggcc
     1201 gacagcggtg ctatgatcac cgccgatttc gccctggaac aggggcgcga ggtctttgcc
     1261 gttcccggca acatcgacag cttcgccagt cgcggctgtc atcagctcat caaagagggc
     1321 gcccacctga tcgagagcgc cgcggatatc ctggatgcgc tcgatctctc caccgccgcg
     1381 cttgaggatg ccccgccaca acagccctct ctcccgtttc cgctcagccc ggaagcggag
     1441 caagtgctcg cgcgcctggg cccgcagcag cgacacgtcg atcgcggtcg aggccgtgaa
     1501 gttcgcgccg gtgctg
//
LOCUS       DAOSCV010001053         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56267_length_1511_cov_2.845467, whole genome shotgun sequence.
ACCESSION   DAOSCV010001053 DAOSCV010000000
VERSION     DAOSCV010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56267_length_1511_cov_2.845467"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1511
                     /locus_tag="PLY56_18985"
     CDS             <1..>1511
                     /locus_tag="PLY56_18985"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ23618.1"
                     /translation="MRAVLADGKVATFGAVSVEEARRRATGDTLEASLYRTIPELVER
                     HREEIRRRFPALQRNVSGYALHAVDVALSEQGTLDLGRLVCGSEGTLAIVTEAKLALV
                     PRPRCVALGVVAFDDVIAALEATVALLDTNPVAIELTDRMILDLAKQSREYSREHIPV
                     SGDPEAILLVEYHGASEAEVAAGLDRMESAMRSGFPRAECVRVPDPADQAKVWKVRKA
                     GVGILLGMPGDRKPIAFVEDTAVEPARLPEYARRFREIVARHGTTAGYYGHASVGCLH
                     LRPLIDIHQRDDVERMKAIASDVADLVREFGGSMTGEHGDGRVRSLWIERMYGPELLG
                     AFREVKRTFDPQGILNPGTIVDAPPMDAHLRPDGARPLKLKTHLSFKREGGILRAVTQ
                     CSGVGICRKRGEGVMCPSYAATGEERLSTRGRANALRAVLAGELPPEELTGEELHEAI
                     SLCLACKGCQNECPSSVDMARLKVEVLAHYYERHGTPPGAAVMGRIDLLSRLG"
BASE COUNT          236 a          505 c          542 g          228 t
ORIGIN      
        1 cgatgcgtgc cgtgcttgcc gatgggaagg tggccacctt cggcgccgtc tcggtggagg
       61 aggcacgccg gcgcgccacg ggcgacacct tggaagcctc tctctaccgg accatcccgg
      121 agctcgtcga acggcatcgg gaggagatcc gtcggcgctt cccggcgctg cagcgcaacg
      181 tctccggcta cgcgctccat gccgtggatg tggcgctctc tgagcagggc acgctcgatc
      241 tgggccgact ggtctgtggc tccgagggca cgctggccat cgtcaccgag gcgaagctgg
      301 cactggtgcc caggccgcgc tgcgtggcgc tcggcgtcgt cgccttcgat gacgtgatcg
      361 ccgcgctgga ggccaccgtt gcccttctcg atacgaaccc ggtcgcgatt gagctgaccg
      421 accggatgat cctcgaccta gcgaaacagt cgcgagagta ctctcgcgag catatcccgg
      481 tctccggcga tccagaggcg atcctcctgg tggagtatca cggggcaagc gaggcagaag
      541 tcgccgccgg gctggaccgg atggagagcg ccatgcgcag cggtttcccg cgagcggagt
      601 gcgtgcgcgt gcccgacccg gcggatcagg cgaaggtgtg gaaagtgcga aaggccggcg
      661 tggggatcct gctgggcatg ccgggagacc gcaagccgat cgccttcgtc gaggataccg
      721 cggtggagcc ggcgcgcctg cccgaatacg cgcgtcgctt ccgcgagatc gtcgcccgac
      781 acggtaccac ggcaggctac tacggccatg ccagcgtcgg gtgcctccac ctgcgcccgc
      841 tcatcgatat tcaccagcgc gacgacgtgg agcggatgaa ggcaatcgcc tcggacgtgg
      901 ccgacctggt gcgcgagttc ggcggttcga tgacgggcga gcatggcgat ggccgggtgc
      961 ggtcgctctg gatcgagcgg atgtacggcc cggagctcct gggcgccttc cgcgaggtga
     1021 agcgcacctt cgatccccag ggcatcctca acccggggac gatcgtcgac gcgccgccga
     1081 tggatgcgca tctgcgcccc gacggcgcgc gaccgctcaa gctgaagaca cacctcagct
     1141 tcaagcgcga gggcggcatc ctgcgcgcgg tgacccagtg cagcggcgtg ggaatctgcc
     1201 gcaagcgggg cgaaggcgtg atgtgtccgt cctacgcggc caccggcgag gagcgcctct
     1261 ccacgcgcgg gcgcgccaac gcgctgcgcg ccgtgctcgc gggagaactt cctcccgagg
     1321 agctcaccgg ggaggagctg cacgaggcga tatcgctctg cctggcgtgc aaggggtgcc
     1381 agaacgaatg cccatcctcg gtggatatgg cccggctgaa ggtggaggtg ctggcacact
     1441 attacgagcg ccacggcacc ccgcccggcg ccgcggtgat gggccggatc gacctcctca
     1501 gccgcctcgg c
//
LOCUS       DAOSCV010001054         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56284_length_1511_cov_2.176511, whole genome shotgun sequence.
ACCESSION   DAOSCV010001054 DAOSCV010000000
VERSION     DAOSCV010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56284_length_1511_cov_2.176511"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLY56_18990"
     CDS             complement(<1..282)
                     /locus_tag="PLY56_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23619.1"
                     /translation="MHPALDLEAKVDAILARADDETLPAVPASLYLDLAESIVRQAAR
                     LQAPDGHVDDLYGPPGVETSTATGRYAGAVAHLLRAGRCLDLLESAMAAL"
     gene            523..669
                     /locus_tag="PLY56_18995"
     CDS             523..669
                     /locus_tag="PLY56_18995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOJ23620.1"
                     /translation="MPARKEGFTLIELLVVIAIIAILAAILFPVFARARENARKSTCQ
                     SNLK"
     gene            688..1164
                     /locus_tag="PLY56_19000"
     CDS             688..1164
                     /locus_tag="PLY56_19000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23621.1"
                     /translation="MYVQDYDEVCFAGNGYQGNSGNYQLIMQPYFKNTNIFTCPSRGF
                     SSFEYGGDGAYGRFTLWYGYSNIFRRGASMAQISAPSETAMVHDGCHPAVDGPRGAVP
                     KVCKGFDPCQTTVINASHFYHSEGSNVAFWDGHVKWYSWQNLSITGNNPPSIWRTN"
BASE COUNT          257 a          485 c          498 g          271 t
ORIGIN      
        1 gagggcagcc atcgcggatt caaggagatc gaggcagcgt ccggcacgca ggaggtgggc
       61 aaccgcgccg gcatagcgcc cggtagcggt ggacgtctcg acgccgggag ggccatagag
      121 atcatccacg tgcccgtccg gcgcctggag ccgggcagcc tgccggacga tgctctccgc
      181 caagtcgagg tagaggctgg ccggcacggc gggcagtgtt tcgtcatcgg cgcgagccaa
      241 gatcgcgtct actttcgctt cgaggtccag cgcggggtgc atcgggcgtt catcctttcg
      301 ctcggtggga ggggcggcag ggtcgccccc ggcttcgccg cggtccgggc gctttcctgc
      361 cgcgcggaaa cgctttccga ggtttctctc gcgtgccagc cgaccgattg ccgcgcgctg
      421 ggtggactct cccgccgcgc tgtgctatac tctcggaaag gacgcggtgt agcgggtgag
      481 cccgctctca tccggcatac ctactacgag aggaggaata cgatgccggc gaggaaagag
      541 ggcttcacgt tgattgagct gctagtcgtg atcgcgatta tcgccatcct ggcggcgatt
      601 ctgtttcctg tctttgcgcg agccagggag aacgcgcgca agtccacctg ccagagcaac
      661 ctgaagtaga tcgcccaagg gatcgcgatg tacgtgcagg actacgacga ggtgtgcttt
      721 gcaggcaacg gctatcaggg caactcgggg aactaccagc tgatcatgca gccgtacttc
      781 aagaacacga acatcttcac atgtcccagc cggggcttct catccttcga gtacggcggc
      841 gatggagctt acggtcggtt cacgctgtgg tatggctact cgaacatctt ccggcgggga
      901 gccagcatgg cgcagatctc ggcgccctcg gagaccgcga tggtccacga tggctgccac
      961 ccggctgttg acggcccccg cggtgccgtg ccgaaggtct gcaagggctt cgatccgtgc
     1021 cagacgacgg ttatcaacgc ttcccatttc taccatagcg aggggagcaa cgtcgccttc
     1081 tgggacggcc acgtcaagtg gtactcctgg cagaacctga gcatcacggg gaataacccg
     1141 ccttccatct ggcgcacgaa ctgaggccat ctggagcgcc gcacgccatc ggcgcactgg
     1201 cgtggcgtgc gagcctgaag acccgcgcgc cacgtccgtg ggggtaggcc catgcgacgc
     1261 ccatccgggc caatcgatgt gcgggttctg atagccctcc caccttctcg gcaaacgccc
     1321 tagggacctc ggctcctccc aggccagcct gggttgcccg gtgggctagc ccaggccgta
     1381 gggttttgcc cacacgcagc acgggtgtcg atgcccgccg cgatctttac gcccgtgcca
     1441 ttctagcccg cattattcat cagaatgatg cgggctaggg cgcgtagcgc cacggcgcgg
     1501 ccacgcccgg c
//
LOCUS       DAOSCV010001055         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56374_length_1509_cov_2.819807, whole genome shotgun sequence.
ACCESSION   DAOSCV010001055 DAOSCV010000000
VERSION     DAOSCV010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56374_length_1509_cov_2.819807"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLY56_19005"
     CDS             complement(<1..292)
                     /locus_tag="PLY56_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23622.1"
                     /translation="MSQSRNGSRKPQTYYELSRTLTYSAAAVLPLLVLYEVGVWWLNF
                     GSPAGVRNAADVALKEPFMLLGPYGQHAFVGLLFLGIVAIYRFETVPKRIRLI"
     gene            768..>1509
                     /locus_tag="PLY56_19010"
     CDS             768..>1509
                     /locus_tag="PLY56_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23623.1"
                     /translation="MKVARFSSARDAVDFAREARAAGWRITLPDAAYPVTWASLRATL
                     LGAVLGGILLGTVGGLGELGLIRLRGLEPLFAAPLLGVVFLLAVAGASLGGLIAALIC
                     LRPVPLLTPGQPYRVCIDGRGNNEAMANLARRYGGSLDGQGSLQTEAAPPPSAREMTL
                     PRWLAWGIAVVTAAVLLIATFYIWLVALFRGPNSNQETRIGYTLKNAQRIPAETPASV
                     GRALSQMMGGPTLAVPEDPIAAAILAPVA"
BASE COUNT          234 a          496 c          506 g          273 t
ORIGIN      
        1 tgatcagccg gatgcgcttg ggcaccgtct cgaagcgata gatcgccacg atgccgagga
       61 agagcaggcc cacgaaggcg tgctgcccgt agggtccgag gagcataaac ggctccttca
      121 acgccacatc ggcagcgttg cgcaccccag ccggcgagcc aaagttgagc caccacaccc
      181 cgacctcata caacaccagg agcggcagca ccgccgccgc ggagtaggtg agcgtcctcg
      241 ataactcgta gtaggtctgg ggcttgcgcg agccgttccg cgattgcgac attcccttct
      301 ctctccgccg ctattctacc acgttgagcc acttccgcca agggcattgc gcccgggccc
      361 acacgcagcg tgcgggcttg cgctcccgca atatcacgcc ggccagcagg cgcgggggtt
      421 tgggcctgcg catgcatcca ggcgggccca gggcggagtg gcctgcctct tgctggttca
      481 tcccgtgccg gatcggcggg aacggctgct ctcgccttcc tgccgccgca agatcgggag
      541 agtgatcgat gctgcatccg tggaatggag ggacgcccgg ctgggaaatc actctggtag
      601 ccatcaggat ctcctttttg aacgccctcc ttgttgggtt ggcgttcctg atccgctcgt
      661 tccgcgcgtg ttcctctgag gagcggctcc ttacgtatca agaggcgcgc tgtgtgcaga
      721 tgaggcggga tcgatggtga acgtctgaac ggcgaggggg aatgctgttg aaagtcgctc
      781 ggttttcatc agccagggac gcggtcgatt tcgcgcggga ggcacgggca gccgggtggc
      841 gtatcacact gccggatgcc gcctatcccg tgacgtgggc ctcactgcgc gcgacactcc
      901 tgggcgcggt cctcggaggc attcttctcg gcacggtggg tggcctcggc gagctggggt
      961 tgattcggct ccggggcctg gaacccctct tcgcggcgcc gctgctgggc gtcgtgttcc
     1021 tcctcgccgt cgccggggca agtcttggcg gactgatcgc cgcattgatc tgcctgcgac
     1081 cggtaccctt gctgaccccc ggacaaccgt accgcgtgtg catcgatggc cgggggaata
     1141 acgaggcgat ggccaacctc gcgcgtcgct acggcggcag cctggatggc cagggcagcc
     1201 tgcaaaccga ggcggccccg ccccccagcg cgcgggagat gactctcccg cgctggctgg
     1261 cgtgggggat cgcggtggta acggcagccg ttctgctcat cgccacgttt tatatctggc
     1321 tcgtggccct cttccgcggt ccaaactcca accaggagac gcgcattggc tacacgctga
     1381 agaacgcgca gcggatcccg gcggagacac ccgcctcggt gggccgtgca ttgtcacaaa
     1441 tgatgggcgg ccccaccctg gcggtgccgg aagacccgat cgcggcggcc attctcgcgc
     1501 cggtggccg
//
LOCUS       DAOSCV010001056         1508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56440_length_1508_cov_2.878183, whole genome shotgun sequence.
ACCESSION   DAOSCV010001056 DAOSCV010000000
VERSION     DAOSCV010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56440_length_1508_cov_2.878183"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1508)
                     /gene="nuoL"
                     /locus_tag="PLY56_19015"
     CDS             complement(<1..>1508)
                     /gene="nuoL"
                     /locus_tag="PLY56_19015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOJ23624.1"
                     /translation="SFAWVPTGDFVLRMGFRLDGLAAIMLLVVTIVATGVQFYSIGYM
                     HGDPRYWRFFAYLSLFSCAMLGLVLANNLLQLYIFWELVGLCSYLLIGFWFERPEAMR
                     AAKKAFLVTRLGDIGFLLGLLILFRHAGTFEYAQLFQRLPEIAAATPMVLTAAALLLF
                     CGAIGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLVGRMYPLFQAATEPWA
                     PSVLGMTLFQATPLQFVAAIGAITAVLGATIGVLQSDIKRVLAFSTISQLGYMMIGLG
                     VGGFFAGLFHLVTHAFFKALLFLGAGSVIHGTGTQDLFEMGGLRKKMPQTFWTFLIGS
                     LSLAGFPLLFSGFWSKEEILLGAFHVSTPIFVAGILGAVLTAFYMTRLVVLAFFGEPR
                     NPEIHAHESPLNMCLPLWVLAVLSIGWWKFVMLPLHHLIDPGHFSAEVEHAGIVQILG
                     IGAGLLGILAGYLVYYRQSPDPRRALIALPLVGERLVPGATGWLGRAPGVYTL"
BASE COUNT          327 a          482 c          512 g          187 t
ORIGIN      
        1 agggtgtaga cgcccggcgc gcgccccagc cagccggtag cccccggcac gagacgctcg
       61 cccaccaacg gcagcgcgat cagcgcccgg cgcgggtcgg ggctctggcg gtagtagacc
      121 aggtagcccg ccagaatgcc gaggaggccg gcgccaattc cgaggatctg gacgatccct
      181 gcgtgctcca cctcagccga gaagtgcccc ggatcgatca ggtggtgcag gggcagcatc
      241 acgaacttcc accacccaat cgagagcacg gcgagcaccc acaacgggag gcacatgttc
      301 aacgggctct cgtgggcatg gatctcgggg ttacgcggct cgccgaagaa ggcgaggacc
      361 accaggcgcg tcatgtagaa cgcggtcaac accgcgccga ggatgccggc cacgaagatc
      421 ggcgtcgaga cgtggaatgc cccgagcagg atctcttcct tgctccagaa cccagagaac
      481 agcagcggga agcctgccag cgagagcgat ccgatcagga acgtccagaa ggtctgcggc
      541 attttcttgc gcaggccgcc catctcgaaa aggtcctggg tgccggtgcc gtggatcacg
      601 ctcccggccc cgaggaagag gagcgccttg aagaaagcgt gcgtcaccag gtggaaaagc
      661 ccggcgaaga agccgcccac gcccaggccg atcatcatgt agccaagctg gctgatcgtc
      721 gagaaggcca gcacgcgctt gatgtcgctc tggagcacgc cgatggtggc cccgagcacc
      781 gccgtgatgg caccaatggc ggcgacgaac tgcaatggcg tcgcctggaa aagcgtcatg
      841 cccagcaccg agggcgccca cggctcggtg gccgcctgga agagcgggta cattcgcccg
      901 accaggtaga cgccggccgc aaccattgtc gccgcgtgga tcagggcgct gaccggcgtg
      961 gggccctcca tggcatcggg aagccacaca tgcagcggga actgcgccga cttgccgatg
     1021 gccccgcaga aaaggaggag cgccgccgcc gtgagcacca tcggcgtggc cgccgcgatc
     1081 tccggcaggc gctggaagag ctgcgcgtac tcgaacgtgc ccgcgtgccg gaagaggatc
     1141 agaagcccta gcaagaagcc gatatcgccc aggcgcgtta ccaggaacgc cttcttggcg
     1201 gcgcgcatcg cctcggggcg ctcgaaccag aacccgatga gcaagtagga gcagaggccc
     1261 accagctccc agaagatgta gagttggagc aggttgttcg ccagcaccag gccgagcatg
     1321 gcgcagctaa agagcgagag ataggcgaag aagcgccaat agcgcggatc gccatgcatg
     1381 tagccgatag agtagaactg cacgccggtg gcgacaatgg tgacgaccag gagcatgatc
     1441 gccgcaaggc catccaggcg gaagcccatg cgcaagacga agtcgccggt gggcacccag
     1501 gcgaagct
//
LOCUS       DAOSCV010001057         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56606_length_1505_cov_2.800000, whole genome shotgun sequence.
ACCESSION   DAOSCV010001057 DAOSCV010000000
VERSION     DAOSCV010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56606_length_1505_cov_2.800000"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLY56_19020"
     CDS             complement(<1..372)
                     /locus_tag="PLY56_19020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ23625.1"
                     /translation="MERCWRFASPAQMLAKAGAIRAHHRFSTVRFLAYVRIHRAVEAR
                     RSIRGRTMDGEMTTASSPTTATRVPVAVCTIAHNEEANIAACIESVVGFAAEMVILDA
                     GSTDRTAAIVREYQARHPEIIL"
     gene            complement(667..>1505)
                     /locus_tag="PLY56_19025"
     CDS             complement(667..>1505)
                     /locus_tag="PLY56_19025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ23626.1"
                     /translation="SQYDLVMKMCQSRGIPVVCSTIFYIEPKTRQFLSYWLKARSPWG
                     THKTVRRMWQHSSLLLPNSEAEAEQVRFVYGVSREQIHVVPNGVEARFAEGNPALFRD
                     KFGIREPFVLNIGRVEDRKNQHRLIQALKGTGLKLVLIGKEWDPEYSALCRREADGNV
                     LFLPPLAHDDPLLASAYAACRVFALPSLVETPGIVALEAGIAGARVITTPIGGGREYF
                     GDHALYVNPYSVSDIRAKVLQAWNSAGDGEALQQHILRHFTWRRVAEKTLEVYERVLQ
                     RK"
BASE COUNT          265 a          477 c          485 g          278 t
ORIGIN      
        1 caggatgatc tccgggtgcc gcgcttggta ctcgcgcacg attgccgcgg tgcggtcggt
       61 gctgccggca tccaggatca ccatctcggc ggcgaagccc accacactct cgatgcaggc
      121 ggcgatattc gcctcctcgt tgtgtgcgat ggtgcagacc gccacgggga cccgcgtcgc
      181 tgtcgttggg ctactggctg ttgtcatctc tccatccatc gtccggccgc ggatactccg
      241 gcgcgcctcc acggcgcggt ggatccggac gtaggccagg aaccggactg tgctgaaacg
      301 atggtgggcg cggatcgcgc ccgccttggc gagcatctgc gccgggctgg cgaatcgcca
      361 gcagcgctcc atccacgccc agttggcctc ggggttgagc agggtgtcgc gacagccatc
      421 cggaagcggt cgcgggcaat cagcatggcg tccgggatca tattcacgaa ggctggcacg
      481 ccagggtaga gcgacgggtg cctctggtaa tcgccatact cctccaggga tcgctgcact
      541 ttgcgcgcga aagtcgtctt cttcccatcg tcccttcgcc caggtagggc tcggagtgga
      601 tgcaagcggt gagcgccgca cctggcaggc accggtcgca caaaggtggg agggtgaagg
      661 agaagctcac ttccgttgaa ggacacgctc atacacttcc agcgtctttt cagccacccg
      721 ccgccaggtg aagtgcctca agatgtgctg ctgcagcgcc tctccatcgc cggcgctgtt
      781 ccatgcctgc agcactttgg cgcggatatc gctcacggaa tagggattca catagagggc
      841 gtggtcgcca aagtactccc ggccgccgcc gatgggcgtg gtgatgacgc gcgcgccagc
      901 aatccccgcc tcgagggcga cgatgcccgg agtctccacg agcgacggca gcgcgaagac
      961 cctgcacgcg gcgtaggcac tggcaagcaa cgggtcgtcg tgggccagcg gggggagaaa
     1021 gaggacgttg ccatccgctt ctcgccggca cagcgccgaa tactccgggt cccactcctt
     1081 gccgatgagc acgagtttga ggcctgttcc cttcagcgcc tggattagcc ggtgctggtt
     1141 cttacggtcc tcaacacgcc cgatgttaag cacgaacggc tcccggatgc caaacttgtc
     1201 tcggaagaga gccgggttgc cctcggcaaa gcgtgcctcc acgccgttgg gtaccacatg
     1261 gatctgctct cgggaaaccc catagacaaa gcgcacctgc tctgcttccg cctcggagtt
     1321 ggggagaagc agcgatgagt gctgccacat ccggcggacg gtcttgtggg ttccccacgg
     1381 cgagcgcgcc ttcagccaat agctgaggaa ctggcgtgtt ttcggctcta tatagaagat
     1441 ggtggagcag acaacaggta tcccacggct ctggcacatc ttcatgacca ggtcgtactg
     1501 cgagt
//
LOCUS       DAOSCV010001058         1504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56646_length_1504_cov_3.757074, whole genome shotgun sequence.
ACCESSION   DAOSCV010001058 DAOSCV010000000
VERSION     DAOSCV010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56646_length_1504_cov_3.757074"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLY56_19030"
     CDS             complement(<1..502)
                     /locus_tag="PLY56_19030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23627.1"
                     /translation="MHKGVVLTALTLMACLPVAARAAVQPAQLFDIGTARDVGMGGAV
                     TAVADDLCMVLWNPAAAALLEGRRIVYSDSFRTEPKSLRFVSYGQSDGGDLTGALSYL
                     RVDSRTYDTKENTGMYSMGHALSDRLIAGANVKYSRYERLGGAKEAFNVDLGLLYGLT
                     DTTALGL"
     gene            complement(748..>1504)
                     /locus_tag="PLY56_19035"
     CDS             complement(748..>1504)
                     /locus_tag="PLY56_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ23628.1"
                     /translation="LYARTELHHSMGLGDLSWSAYGSFLPETPGSMQSRLYFRMRPGV
                     IKGPNIRYYWSTNVAWNKWGDESYLEEGVDLQLHPPALRLGQTTSLQFYIGSGFTFSP
                     NGTGPNAQASTTLLKRLGKNATATLRYSYYYSGRTTDYYGPTSGQSLTGQLMAASGNR
                     WSTSLTATVLPTRGDLSVYGSLVYSPLRNWRVELRPTYSRYGGDLLAGYPRSTTNLDV
                     YLVRQFGSRDVALRYSTLDDAIRLELAATALRF"
BASE COUNT          266 a          508 c          457 g          273 t
ORIGIN      
        1 caaggccaag cgccgtcgta tcggttagcc catacagcaa gccgaggtct acgttgaacg
       61 cttccttagc gccaccgaga cgctcatacc ggctatactt gacattggcg ccggcaatga
      121 ggcggtcgct caacgcgtgc cccatgctat acatccccgt attctctttt gtgtcgtacg
      181 tccggctatc gacccgcagg tagctcagcg cgcccgtcag atccccccca tccgattgcc
      241 cgtaggagac gaagcgaagg ctcttgggct ccgttcggaa cgagtcgctg tagacgatgc
      301 ggcgaccttc caagagcgca gcggcggccg gattccacag gaccatacag aggtcatctg
      361 ccaccgccgt aaccgctccg cccatgccca cgtcgcgcgc agtgccgata tcaaagagct
      421 gggcaggctg gaccgccgcc cgggcggcta ccgggaggca cgccatgagg gtcagggcag
      481 tcagaacgac tcctttgtgc atcgtggtct tgctccttat cgtttgcttc cttcagtccc
      541 cgtcggtggc aagcgcgggc gcgatcctct tcacgggagg cgccgccggt gaacccggtg
      601 gccgcccccg cgtcttgcca tgcaggccgc gctcccgccg ggcgctttgc cccctggatg
      661 aggcctgggc gacgacatgg gcacccttgc ctagttcgcc acgcgcgtcc atcaacctgc
      721 taaccgcctg ccctgcgcct gcttccctca gaaacgcagc gccgttgccg ccagctccag
      781 gcggatcgcg tcatctagcg tggaatagcg gagcgccaca tcgcgcgacc caaactgccg
      841 cacgaggtag acatccaggt tcgtcgtgct ccgcggatac cccgccaaca agtcgcctcc
      901 gtaccgcgag tatgttgggc gaagctccac gcgccagttg cgtagcgggg aatagaccag
      961 ggacccatac accgacaggt caccccgcgt cggaagcacc gtggccgtga gcgaggtgga
     1021 ccagcggttc ccgctggcgg ccatcaactg ccccgtgagg ctctgcccgg atgtcgggcc
     1081 ataatagtcc gtcgttcgcc cggagtagta gtagctatag cgcagtgtcg ccgtcgcgtt
     1141 tttgcccagg cgcttcagca aagtggtgga tgcctgcgcg ttggggcccg tgccgttcgg
     1201 tgagaacgtg aagccgctgc cgatgtaaaa ctgaaggctt gtcgtctggc caaggcgcag
     1261 cgcgggcggg tggagctgga gatccacgcc ctcctcaagg taagactcat cgccccactt
     1321 gttccacgcc acgttggtgc tccagtaata ccggatattc ggccccttga tcacccccgg
     1381 gcgcatccgg aaatagagcc tggattgcat tgagcccggc gtctccggga gaaagctgcc
     1441 atatgcgctc cagctcaggt caccgaggcc catcgagtgg tgcaactccg tgcgcgcata
     1501 gaga
//
LOCUS       DAOSCV010001059         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Armatimonadota bacterium isolate AMR_MDS_1848
            NODE_56882_length_1500_cov_3.208997, whole genome shotgun sequence.
ACCESSION   DAOSCV010001059 DAOSCV010000000
VERSION     DAOSCV010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863377
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Armatimonadota bacterium (wastewater metagenome)
  ORGANISM  Armatimonadota bacterium
            Bacteria; Bacillati; Armatimonadota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:01:14
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,809
            CDSs (total)                      :: 3,770
            Genes (coding)                    :: 3,735
            CDSs (with protein)               :: 3,735
            Genes (RNA)                       :: 39
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 3 of 35
            Pseudo Genes (incomplete)         :: 29 of 35
            Pseudo Genes (internal stop)      :: 3 of 35
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Armatimonadota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56882_length_1500_cov_3.208997"
                     /isolate="AMR_MDS_1848"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2033014"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PLY56_19040"
     CDS             <1..840
                     /locus_tag="PLY56_19040"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOJ23629.1"
                     /translation="MFRPIFEASGYKEGYLSGQVDPRILQDTDAMVKQGVELNNMAKN
                     VMIKMPGTKEGIEGIRRLTAMGIPTNATLTFTVSQLVAVAEAVKAGLAEARAKGVDLS
                     KWRSVVTMMLGRMEDAKVFAEQAASLGIELSETDKRWAGVAVFKKIYRIYKERGYESK
                     ELAASMRLGPTIDGKLSIWHIEKLAGMDVVLTVFPNIFEGWLNAYADREIVSNEGEEV
                     PAEVLEKLLKVPYFRQAYDENGLAPEEFINQPAVVETKTQFNAATDEFEAYCASRIKA
                     VKG"
     gene            1024..>1500
                     /locus_tag="PLY56_19045"
     CDS             1024..>1500
                     /locus_tag="PLY56_19045"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ23630.1"
                     /translation="MSRATQIAAGTGLTLAGVAGLNALVQSSARKLPNALKEGERHTY
                     EWRYGRVSYQVAGEGQPLLLIHGVYAGASNFEWRHNFGALADNFRAYAIDLLGFGRSD
                     RPTITYTDDLYTDLIHDFVRDVVADPAFAIASSVSASFLVADAAATPDAYRGLILIC"
BASE COUNT          276 a          487 c          488 g          249 t
ORIGIN      
        1 atgttccgcc cgatcttcga ggcgtccggg tacaaggagg gctacctctc cggccaggtc
       61 gatcctcgca tccttcagga caccgatgcc atggtcaagc agggtgtcga gctgaacaac
      121 atggcgaaga acgtcatgat caagatgccc ggcaccaagg aaggcatcga gggcatccgc
      181 cggctgaccg cgatgggcat cccgacgaac gcgacgctca ccttcacggt gtcgcagctc
      241 gtcgccgtcg ccgaggcggt gaaggcgggc cttgccgagg cgcgcgccaa gggcgtggac
      301 ctcagcaagt ggcgctccgt cgtcaccatg atgctcgggc gcatggaaga cgcgaaggtg
      361 ttcgccgagc aggctgcctc gctcgggatc gagctgtcgg agaccgacaa gcgctgggca
      421 ggcgtcgccg tcttcaagaa gatctaccgg atctacaagg agcgcggcta cgagagcaag
      481 gagctcgccg cttcgatgcg cctcggcccg acgatcgacg gcaagctcag catctggcac
      541 atcgagaagc tcgccggcat ggacgtcgtc ctcaccgtct tcccgaacat cttcgagggc
      601 tggctcaacg cctacgccga ccgcgagatc gtgagcaacg agggcgagga agtgcccgct
      661 gaggtgctcg agaagctcct gaaggttccg tacttccgcc aggcatacga cgagaacggc
      721 ttggcgcccg aagagttcat caaccagccc gccgtggtcg agacgaagac ccaattcaac
      781 gcggcgactg atgagttcga ggcctactgc gcgtcgcgca tcaaggccgt caagggctaa
      841 tctcgctggt agttcagcgg accgcttgag ggggagcggg tgccacgcac ccgctccccc
      901 tttctctgtg gcgcgcgccg aatctgacag caaagcaggg ttagcctggg ccaccgcggg
      961 ggagattccg cgggggagcg cgtcacggcc ctccgcggac ccggtcgctc gaggaggaga
     1021 agagtgtcac gtgcgactca gatcgcggcg ggaacaggac tgaccctggc gggcgtggcc
     1081 ggcctcaacg cgctggtcca gtcttccgct cgaaagctgc ccaacgcctt gaaggaaggc
     1141 gagcgccata cctatgagtg gcgctacggg cgcgtcagct accaggtggc gggcgagggc
     1201 cagccgttgc tgctcatcca cggcgtgtac gccggcgcca gcaacttcga gtggcgccac
     1261 aacttcgggg cactggccga caacttccgg gcctacgcca tcgatctgct gggttttggc
     1321 cgttccgacc gcccgaccat cacctacacc gatgaccttt acacggatct gatccatgat
     1381 ttcgtgcgcg atgtggtcgc ggatcccgct ttcgcgattg ccagctccgt gagcgccagc
     1441 tttctggtgg ccgatgccgc cgccactccc gatgcctatc gcgggctgat cctcatctgc
//
